WorldWideScience

Sample records for biocenosis

  1. [Colonic microbial biocenosis in rheumatoid arthritis].

    Science.gov (United States)

    Gul'neva, M Iu; Noskov, S M

    2011-01-01

    The aim of the work was to study colonic microbial biocenosis and colonizing ability of opportunistic bacteria in 32 patients with rheumatoid arthritis (RA) and 30 healthy subjects. RA was diagnosed based on the American Rheumatism Association criteria (1987). Qualitative and quantitative composition of the microflora was detected by a bacteriological method. StatSoft Statistics 6.0 was used to treat the data obtained. RA was associated with significant modification of the intestinal flora, viz. decrease in lactobacteria and significant increase of enterococci, clostridia, colibacteria showing reduced enzymatic activity, and opportunistic species. Also, symbiotic relationships between microorganisms altered. The fraction of bifidobacteria, bacteroids, and lactopositive colibacteria reduced while the abundance of opportunistic enterobacteria and staphylococci was elevated. Opportunistic Enterobacteriaceae were present in urine and nasal mucosa which suggested their translocation from the intestines. It is concluded that changes in intestinal microflora and colonization by opportunistic bacteria enhance the risk of development of co-morbid conditions in patients with RA. PMID:21932563

  2. [The characteristic of biocenosis of urogenital tract in women].

    Science.gov (United States)

    Andosova, L D; Kontorshchikova, K N; Kachalina, O V; Belov, A V; Gonova, E S; Kudel'kina, S Iu

    2013-01-01

    The article deals with the study of characteristics of biocenosis of urogenital tract in women of reproductive age with using of "Femoflor" test. The scrapings of posterolateral wall of vagina were analyzed using the technique of real-time polymerase chain reaction using the reagents "Femoflor". The complex evaluation of urogenital biota identified three main types of biocenosis of vagina: type I--normocenosis (n = 50 or 11.5%): type II--mild dysbiosis (n = 88 or 21%); type III--marked dysbiosis (n = 133 or 30.6%). In the structure of alterations of biocenosis of urogenital tract the main role play the anaerobic bacteria with involvement of candida, ureoplasma and mcoplasma. PMID:23807998

  3. [Experimental simulation of the structure of gastrointestinal microbial biocenosis].

    Science.gov (United States)

    Bogdanova, E A; Nesvizhinskiĭ, Iu V; Korolev, A A

    2010-01-01

    Structural analysis of human and rat gastrointestinal microbial communities revealed their general similarity. The structure of microbial biocenosis in ileum parietal mucin appears to be highly sensitive to nutritional factors. Inadequate nutrition leads to destruction of microbial microassociations in parietal mucin, a calcium-deficient diet has similar effect in feces. Fiber-rich diets stimulates build-up of indigenous communities while artificial nutrients and calcium-enriched diets promote formation of mixed indigenous-transient microbial associations. Bacteria themselves prove to be weak modifiers of the observed effects. PMID:21395060

  4. Studies on yeasts and yeast-like microorganisms in the denitrification unit biocenosis

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    Elena Sláviková

    2014-08-01

    Full Text Available It was found that Candida famata, Hansenula californica and Rhodotorula rubra occurred in reactor UASB-type biocenosis in the course of denitrification carried out in the presence of lactic acid as a carbon source. The role of those species in nitrogen removal process was discussed with respect to their physiology.

  5. The yeasts and yeast-like microorganisms in the denitrification unit biocenosis

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    Alena Sláviková

    2014-08-01

    Full Text Available Taxonomic studies of the yeasts and yeast-like microorganisms in the denitrification unit biocenosis were carried out. A set of 13 strains of these microorganisms were examined for their morphological and physiological characters. Considering their special features and some relation to the known species, the isolated microorganisms were classified to the 3 genera: Candida, Geotrichium and Hansenula.

  6. Taxonomical study of yeasts and yeast-like microorganisms isolated from the denitrification unit biocenosis

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    Elena Sláviková

    2014-08-01

    Full Text Available A set of 8 strains of yeasts and yeast-like microorganisms was isolated from the denitrification unit biocenosis fed with a synthetic medium containing methanol as a carbon source. These strains were identified as Candida boidinii, C. maltosa, Rhodotorula rubra and Trichosporon cutaneum.

  7. Taxonomical study of yeasts and yeast-like microorganisms isolated from the denitrification unit biocenosis

    OpenAIRE

    Elena Sláviková; Anna Grabińska-Łoniewska

    2014-01-01

    A set of 8 strains of yeasts and yeast-like microorganisms was isolated from the denitrification unit biocenosis fed with a synthetic medium containing methanol as a carbon source. These strains were identified as Candida boidinii, C. maltosa, Rhodotorula rubra and Trichosporon cutaneum.

  8. [Biocenosis sparing treatment in the surgery of gastro-intestinal tract].

    Science.gov (United States)

    Lazarev, S M; Voronina, O V

    2009-01-01

    The authors present results of investigation of the state of microbiocenosis of the intestine and immune status of organism of 139 patients after emergency operations on the gastro-intestinal tract organs. Disbiosis and reduced indices of the immune system in the postoperative period was a cause of using biocenosis sparing treatment resulting in stabilization of microecology of the gastro-intestinal tract, higher colonization resistance and general reactivity of organism. PMID:19432155

  9. Effect of prolonged waterflooding of oil deposits on developing biocenosis and layer microflora activity

    Energy Technology Data Exchange (ETDEWEB)

    Berdichevskaya, M.V.

    1982-01-01

    It is shown that prolonged waterflooding of the oil layer with fresh water forms conditions for the spread and subsequent development of biocenosis of hydrocarbonaceous acid, sulpha-forming and methane-forming bacteria. The activity of the layer microflora under near natural conditions are determined. The 24 hour growth of bacterial biomass depending on such ecological factors as salinity and acidic-forming conditions varies from 0.138 to 0.408 mkM C/1. The time it takes for the bacterial biomass to double is given. Under varying ecologic conditions, it is from 18 to 120 h.

  10. [Deterministic analysis as a tool to investigate the contingency of various components of biocenosis].

    Science.gov (United States)

    Bulgakov, N G; Maksimov, V N

    2005-01-01

    Specific application of deterministic analysis to investigate the contingencies of various components of natural biocenosis was illustrated by the example of fish production and biomass of phyto- and zooplankton. Deterministic analysis confirms the theoretic assumptions on food preferences of herbivorous fish: both silver and bighead carps avoided feeding on cyanobacteria. Being a facultative phytoplankton feeder, silver carp preferred microalgae to zooplankton. Deterministic analysis allowed us to demonstrate the contingency of the mean biomass of phyto- and zooplankton during both the whole fish production cycle and the individual periods. PMID:16004266

  11. A highly diverse, desert-like microbial biocenosis on solar panels in a Mediterranean city

    Science.gov (United States)

    Dorado-Morales, Pedro; Vilanova, Cristina; Peretó, Juli; Codoñer, Francisco M.; Ramón, Daniel; Porcar, Manuel

    2016-01-01

    Microorganisms colonize a wide range of natural and artificial environments although there are hardly any data on the microbial ecology of one the most widespread man-made extreme structures: solar panels. Here we show that solar panels in a Mediterranean city (Valencia, Spain) harbor a highly diverse microbial community with more than 500 different species per panel, most of which belong to drought-, heat- and radiation-adapted bacterial genera, and sun-irradiation adapted epiphytic fungi. The taxonomic and functional profiles of this microbial community and the characterization of selected culturable bacteria reveal the existence of a diverse mesophilic microbial community on the panels’ surface. This biocenosis proved to be more similar to the ones inhabiting deserts than to any human or urban microbial ecosystem. This unique microbial community shows different day/night proteomic profiles; it is dominated by reddish pigment- and sphingolipid-producers, and is adapted to withstand circadian cycles of high temperatures, desiccation and solar radiation. PMID:27378552

  12. A highly diverse, desert-like microbial biocenosis on solar panels in a Mediterranean city.

    Science.gov (United States)

    Dorado-Morales, Pedro; Vilanova, Cristina; Peretó, Juli; Codoñer, Francisco M; Ramón, Daniel; Porcar, Manuel

    2016-01-01

    Microorganisms colonize a wide range of natural and artificial environments although there are hardly any data on the microbial ecology of one the most widespread man-made extreme structures: solar panels. Here we show that solar panels in a Mediterranean city (Valencia, Spain) harbor a highly diverse microbial community with more than 500 different species per panel, most of which belong to drought-, heat- and radiation-adapted bacterial genera, and sun-irradiation adapted epiphytic fungi. The taxonomic and functional profiles of this microbial community and the characterization of selected culturable bacteria reveal the existence of a diverse mesophilic microbial community on the panels' surface. This biocenosis proved to be more similar to the ones inhabiting deserts than to any human or urban microbial ecosystem. This unique microbial community shows different day/night proteomic profiles; it is dominated by reddish pigment- and sphingolipid-producers, and is adapted to withstand circadian cycles of high temperatures, desiccation and solar radiation. PMID:27378552

  13. Methods for evaluating the pollution impact of urban wet weather discharges on biocenosis: A review.

    Science.gov (United States)

    Gosset, Antoine; Ferro, Yannis; Durrieu, Claude

    2016-02-01

    Rainwater becomes loaded with a large number of pollutants when in contact with the atmosphere and urban surfaces. These pollutants (such as metals, pesticides, PAHs, PCBs) reduce the quality of water bodies. As it is now acknowledged that physico-chemical analyses alone are insufficient for identifying an ecological impact, these analyses are frequently completed or replaced by impact studies communities living in freshwater ecosystems (requiring biological indices), ecotoxicological studies, etc. Thus, different monitoring strategies have been developed over recent decades aimed at evaluating the impact of the pollution brought by urban wet weather discharges on the biocenosis of receiving aquatic ecosystems. The purpose of this review is to establish a synthetic and critical view of these different methods used, to define their advantages and disadvantages, and to provide recommendations for futures researches. Although studies on aquatic communities are used efficiently, notably on benthic macroinvertebrates, they are difficult to interpret. In addition, despite the fact that certain bioassays lack representativeness, the literature at present appears meagre regarding ecotoxicological studies conducted in situ. However, new tools for studying urban wet weather discharges have emerged, namely biosensors. The advantages of biosensors are that they allow monitoring the impact of discharges in situ and continuously. However, only one study on this subject has been identified so far, making it necessary to perform further research in this direction. PMID:26720196

  14. Microscopy and Molecular Biology Techniques for the Study of Biocenosis Diversity in Semi-Confined Environments

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    Franco Palla

    2010-12-01

    Full Text Available This study is part of a wider conservation project of artistic and anthropological finds located in the Grotto of the Saints (Licodia Eubea, Alia, Sicily, and represents an opportunity for  investigating the micro-and macro biological systems colonizing this particular environment. It is well-known that the  bio-receptivity of surfaces is strongly related to its constituent materials and environmental parameters, whose effects promote the establishment of specific biotic communities. This is particularly true for caves, hypogea and semi-confined environments and, in particular for the Grotto of the Saints, where besides the presence of different nutrient sources, there are also high humidity values, percolating water and an aerobiological exchange with the surrounding countryside. Moreover, the weathering of this structure is enhanced by the canyon effect of the wind and the day-night temperature range. The identification and characterization of the biocenosis present in this environment was performed combining microscopy (optical, fluorescent, CLSM and molecular biology analysis (DNA sequences. The aim was to identify the biological systems able to trigger the degradation processes, in order to plan their growth control and to prevent the colonization of the entire environment.

  15. Characterization of biocenosis in the storage-reservoirs of liquid radioactive wastes of 'Mayak' PA

    International Nuclear Information System (INIS)

    A number of storage-reservoirs of liquid radioactive wastes of 'Mayak' Production Association ('Mayak' PA) with different levels of radioactive contamination: reservoir R-17 ('Staroye Boloto'), reservoir R-9 (Lake Karachay), reservoirs of the Techa Cascade R-3 (Koksharov pond), R-4 (Metlinsky pond), R-10 and R-11 is located in Chelyabinsk Oblast (Russia). The operation of these reservoirs began in 1949-1964. Full-scale hydro-biological studies of these reservoirs were started in 2007. The research into the status of biocenosis of these storage reservoirs of liquid radioactive wastes of 'Mayak' PA was performed in 2007 - 2011. The status of biocenosis was evaluated in accordance with the status of following communities: bacterio-plankton, phytoplankton, zooplankton, zoo-benthos, macrophytes and ichthyofauna. The status of ecosystems was determined by radioactive and chemical contamination of water bodies. The results of hydro-biological investigations showed that no changes in the status of biota in reservoir R-11 were revealed as compared to the biological parameters of the water bodies of this geographical zone. In terms of biological parameters the status of the ecosystem of the reservoir R-11 is characterized by a sufficient biological diversity, and can be considered acceptable. The ecosystem of the reservoir R-10 maintains its functional integrity, although there were registered negative effects in the zoo-benthos community associated with the decrease in the parameters of the development of pelophylic mollusks that live at the bottom of the water body throughout the entire life cycle. In reservoir R-4 the parameters of the development of phytoplankton did not differ from those in Reservoirs R-11 and R-10; however, a significant reduction in the quantity of Cladocera and Copepoda was registered in the zooplankton community, while in the zoo-benthos there were no small mollusks that live aground throughout the entire life cycle. In reservoir R-3 there was no

  16. The role of the contribution of the whole sea energy on pollution distribution and biocenosis quality: a case study in the Northern Tyrrhenian Sea, Italy

    Science.gov (United States)

    Bonamano, Simone; Piermattei, Viviana; Piazzolla, Daniele; Paladini de Mendoza, Francesco; Manfredi Frattarelli, Francesco; Mancini, Emanuele; Cognetti de Martiis, Selvaggia; Madonia, Alice; Martellucci, Riccardo; Stefanì, Chiara; Pierattini, Alberto; Scanu, Sergio; Marcelli, Marco

    2016-04-01

    Marine sediments and benthic biocenosis record, at different spatial and temporal scales, natural and anthropogenic processes that occur in the water column. The coastal area of Civitavecchia, in the Tyrrhenian Sea, Italy, presents valuable marine and coastal ecosystems, which are subject to pollution due to the presence of potentially impactful uses of the coastline (the most important port for traffic passenger in the Mediterranean sea and one of the biggest energy production site in Europe) that overlap with the presence of natural geogenic anomalies related to the concentrations of some trace elements. The C-CEMS monitoring system, currently available in the study area, is able to perform the analysis of pollutants dispersion in coastal waters using in situ and remote observations coupled with numerical models simulations . In particular, water column parameters trends and the distribution of both benthic biocenosis and pollutants from natural sources and human activities in the water column can be assessed. This work focuses on the distribution patterns of pollutants and its relationship with the distribution of the benthic community in relation to biocenosis. Moreover, this work presents a first attempt to relate the whole sea energy contribution, which has effects on both the deposition of fine material and pollutants associated with it, and the distribution of communities, in terms of "exosomatic energy" (sensu Margalef).

  17. Biocenosis de foraminíferos bentónicos en la zona intermareal de Bocagrande Cartagena (Colombia

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    Londoño de Hoyos Concha

    1992-11-01

    Full Text Available A comunity composed of 40 individuals is identified at Bocagrande shore, Cartagena. Nine benthic foraminiferal species comform the biocenosia. Siatematically, theyare included in the suborders MILIOLINA and ROTALIINA, and in the families MILIOLIIDAE of the first soborder; and DISCORBIDAE,
    GLABRATELLIDAE, EPISTOMARIIDAE, ROTALIIDAE and NONIONIDAE, belonging to the second suborder. The main features of the faunal assemblage are: the poverty of species and specimens and the dominance of Paratolia magdalensis Lankford.Se identifica una comunidad formada por 40 individuos en la playa de Bocagrande, Cartagena. La biocenosis está conformada por 9 especies de foraminiferos bentónicos. Sistemáticamente se ubican en los subordenes MILIOLINA y ROTALLINA; correspondiendo a las familias MILIOLIDAE del primero y DISCORBIDAE, GLABRATELLIDAE, ROTALIIDAE, EPISTOMARIIDAE y NONIONIDAE, del segundo. Las características principales del conjunto faunístico son: la pobreza, tanto de especies como de especímenes y la dominancia de Pararotolia magdalenensis Lankford.

  18. Characterization of biocenosis in the storage-reservoirs of liquid radioactive wastes of 'Mayak' PA

    Energy Technology Data Exchange (ETDEWEB)

    Pryakhin, E.; Tryapitsina, G.; Andreyev, S.; Akleyev, A. [Urals Research Center for Radiation Medicine - URCRM (Russian Federation); Mokrov, Y.; Ivanov, I. [Mayak PA (Russian Federation)

    2014-07-01

    A number of storage-reservoirs of liquid radioactive wastes of 'Mayak' Production Association ('Mayak' PA) with different levels of radioactive contamination: reservoir R-17 ('Staroye Boloto'), reservoir R-9 (Lake Karachay), reservoirs of the Techa Cascade R-3 (Koksharov pond), R-4 (Metlinsky pond), R-10 and R-11 is located in Chelyabinsk Oblast (Russia). The operation of these reservoirs began in 1949-1964. Full-scale hydro-biological studies of these reservoirs were started in 2007. The research into the status of biocenosis of these storage reservoirs of liquid radioactive wastes of 'Mayak' PA was performed in 2007 - 2011. The status of biocenosis was evaluated in accordance with the status of following communities: bacterio-plankton, phytoplankton, zooplankton, zoo-benthos, macrophytes and ichthyofauna. The status of ecosystems was determined by radioactive and chemical contamination of water bodies. The results of hydro-biological investigations showed that no changes in the status of biota in reservoir R-11 were revealed as compared to the biological parameters of the water bodies of this geographical zone. In terms of biological parameters the status of the ecosystem of the reservoir R-11 is characterized by a sufficient biological diversity, and can be considered acceptable. The ecosystem of the reservoir R-10 maintains its functional integrity, although there were registered negative effects in the zoo-benthos community associated with the decrease in the parameters of the development of pelophylic mollusks that live at the bottom of the water body throughout the entire life cycle. In reservoir R-4 the parameters of the development of phytoplankton did not differ from those in Reservoirs R-11 and R-10; however, a significant reduction in the quantity of Cladocera and Copepoda was registered in the zooplankton community, while in the zoo-benthos there were no small mollusks that live aground throughout the entire life

  19. THE INFLUENCE OF THE CONTENT OF ALUMINIUM ON THE BIOCENOSIS OF THE WATERS OF LAKES WITH POORLY URBANIZED RECEPTION BASINS

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    Jacek Kubiak

    2014-10-01

    Full Text Available The article presents the research results of the content of aluminium in the waters of the largest lakes situated in the reception basin of the Tywa river. The general content of aluminium and its form: dissolved and non-dissolved was examined. The general content of aluminium in those waters varied from 5.3 to 98.9 μg/l, while the content of dissolved aluminium varied from 3.0 to 57.0 and its non-dissolved form from 1.0 to 54.0 μg/l. The average concentration of the content of aluminium in the waters of examined lakes was similar; Dłużec lake – 42.9, Strzeszowskie lake – 39.2, Dołgie lake 45.7, Swobnickie lake 41.4 μg/l. The prevailing form of aluminium in the examined bodies of water was the dissolved form. The greatest amounts of that metal in waters of the examined lakes were present in autumn and spring, and the smallest amounts in summer and winter, the tendency concerned the entire content of aluminium and its non-dissolved form. The dissolved form of aluminium in the waters of examined lakes was present in the largest amounts in winter (on average – 17.4 μg/l, in the smallest amounts in spring (14.0 μg/l, this seasonal diversity – was weakly marked. The existing concentration of aluminium is typical of non-polluted surface waters, and with the stated reaction and the content of sulfurs, carbonates and chlorides in the examined waters, they were not toxic to the biocenosis.

  20. [The assessment of biocenosis contamination in the region of radioactive waste storage (RWS) placing and influence of this storage on rodents].

    Science.gov (United States)

    Vasil'ev, A N; Synzynys, B I; Ul'ianova, L P; Kovalev, O A; Smoryzanova, O A; Starkov, O V; Latynova, N E; Kruglov, S V; Momot, O A; Koz'min, G V

    2007-01-01

    The results of radiative and of chemical monitoring show definite contamination of this zone by 90Sr and toxic metals. The essential local contaminations of geosystems (up to 2.3 x 10(4) Bk/kg of soil) require in environmental condition assessment at biocenosis level. Biotesting found the increase of metallothioneines levels in kidney (up to 15.63 microg/g of tissue) and liver (up to 19.22 microg/g of tissue) of rodents inhabited in the region of RWS placing as compared with the control group (3.51 and 4.44 microg/g of tissue accordingly). Besides, the decrease of total quantity of leucocytes (by 14.5% as compared with the control group) and absolute quantity all forms of them in animal blood were noted. It was assumed the increase of protein--MT is the result of complex influence by ionizing radiation and toxic metals. PMID:18051689

  1. 扎陵湖-鄂陵湖自然保护分区湿地生物群落结构调查%Research on Structure of Wetland Biocenosis in the Gyaring Lake and Ngoring Lake Natural Reserve

    Institute of Scientific and Technical Information of China (English)

    段培; 鲍敏; 张营; 马继雄; 马永贵; 陈振宁

    2015-01-01

    The Gyaring Lake and Ngoring Lake Natural Reserve has kept a record of having 88 species wild animals ,including 51 kinds of birds ,29 kinds of mammals ,1 kind of reptiles and 7 kinds of fish .Among all the species of animals ,there are four kinds of the first nationally protected animals and 13 kinds of the second nationally protected animals .The protected region also contains 17 vegetation species which belong to 6 or‐ders and 8 families ,in which Gramineae ,Compositae and Rosaceae are dominant species .Through the analy‐sis of Shannon-wiener diversity index ,Pielou evenness index and Jaccard index of structures of three bioce‐nosis in different soil environments which include intermittent watered meadow soil ,meadow soil without water and sand without water ,the article finds that the diversity and evenness of biocenosis in sand without water are the highest ,and the similarity of structure of biocenosis in intermittent watered meadow soil and meadow soil without water is the highest .But the similarity of structure of biocenosis in intermittent wa‐tered meadow soil and sand without water is the lowest .In plant community ,the proportion of Gramineae reduces gradually while the dicotyledonous weed increases gradually .In animal community ,the quantity of wetland birds reduces gradually ,while the quantity of other birds and mammals increases gradually .%指出了扎陵湖-鄂陵湖自然保护分区共记录有野生动物88种,其中鸟类51种,兽类29种,爬行类1种,鱼类7种,含国家Ⅰ级保护动物4种、Ⅱ级保护动物13种;植被种类共计17种,隶属于6目8科,其中以禾本科(Gramineae)、菊科(Compositae)和蔷薇科(Rosaceae)植物为优势种。分析了间歇性积水草甸土、无积水草甸土和无积水沙土3个不同土壤环境生物群落结构的 Shannon-w iener多样性指数、Pielou均匀性指数和 Jaccard指数,结果表明:无积水沙土生物群落多样性和均匀度

  2. INFLUENCE PROBIOTICS ON BEGINES OF THE INTESTINAL BIOCENOSIS IN SUCLING PIGLETS Влияние пробиотиков на становление кишечного биоценоза у поросят-сосунов

    OpenAIRE

    Ostrickova E. E.

    2011-01-01

    The article gives an account results of comparetic evaluate of same probiotics on intestinal biocenosis development of suckling piglets. It is establish, that investigated preparates enlarge the concentration of lacto- and biphidobacterium, and reduce the quantity of Escherichia coli group bacterium

  3. INFLUENCE PROBIOTICS ON BEGINES OF THE INTESTINAL BIOCENOSIS IN SUCLING PIGLETS Влияние пробиотиков на становление кишечного биоценоза у поросят-сосунов

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    Ostrickova E. E.

    2011-12-01

    Full Text Available The article gives an account results of comparetic evaluate of same probiotics on intestinal biocenosis development of suckling piglets. It is establish, that investigated preparates enlarge the concentration of lacto- and biphidobacterium, and reduce the quantity of Escherichia coli group bacterium

  4. 升钟水库富营养状况及生物群落调查评价%Investigation and Assessment of Eutrophication Status and Biocenosis in Shengzhong Reservoir

    Institute of Scientific and Technical Information of China (English)

    于飞; 何冬琼; 张秋劲; 刘佳; 徐亮

    2012-01-01

    根据2008~2009年对升钟水库的逐月监测结果,采用综合营养状态指数法,对湖泊富营养化现状进行了评价,并对水库的浮游植物群落种类和密度进行了定性定量分析。结果表明,水库目前处于中营养状态,但在初夏和秋季由于温度和降水等气象条件的影响,可达到轻度富营养,其程度随季节变化明显:初夏和初秋形成高峰,冬、春季为低谷。从生物群落分析,浮游植物种类及密度在不同季节差别较大,初春及夏季的较其他季节多、冬季最低,优势种为蓝绿藻和硅藻。%Based to the monthly monitoring data of water quality in Shengzhong Reservoir during 2008 - 2009, a comprehensive nutrition index assessment is conducted, and the phytoplankton species and density are analyzed quantitatively as well. The results show that the reservoir is now at medium eutrophication level. However, it may be at light level of eutrophication in early summer and autumn significantly depending on the meteorological conditions such as temperature and precipitation. According to the analysis of biocenosis, the species and density of phytoplankton change very much seasonally, higher in early spring and summer and lowest in winter. The blue-green algae and diatom are dominant species.

  5. Preventive biocenosis of oil strata and combatting microbiological corrosion

    Energy Technology Data Exchange (ETDEWEB)

    Asfandiyarov, F.A.; Kildibekov, I.G.; Nizamov, K.R.

    1984-01-01

    The following conclusions are drawn on the basis of the conducted studies: the use of chemical reagents (bactericides or bacterial corrosion inhibitors) is a rational way to combat sulfate reduction in oil strata and microbiological corrosion; the use of bactericides in the early stage of development is an effective method for sterilizing the pumped water to prevent contamination of the productive strata with sulfate reducing bacteria (SVB) and microbiological corrosion and the use of bacterial corrosion inhibitors is an effective method for suppressing microbiological corrosion and reducing sulfate reduction in the later stage of development.

  6. Biocenosis de Bivalvia y Polyplacophora del intermareal rocoso en playa Tlacopanocha, Acapulco, Guerrero, México Polyplacophora and Bivalvia biocenosis at rocky intertidal Tlacopanocha beach, Acapulco, Guerrero, Mexico

    Directory of Open Access Journals (Sweden)

    Lizeth Galeana-Rebolledo

    2012-11-01

    Full Text Available El Estado de Guerrero, México, tiene regiones marinas prioritarias para la conservación de su biodiversidad y la información acerca de la diversidad de moluscos es escasa, por lo que es necesario realizar inventarios de especies, estudios de ecología de poblaciones y comunidades. Este estudio se efectuó en el intermareal rocoso de la playa Tlacopanocha. Los objetivos fueron: determinar la riqueza, densidad y diversidad de especies; analizar la composición de especies y estructura de tallas. El área de muestreo fue de 10 m² y la unidad de muestreó fue de 1 m². Se identificaron 35 especies, de las cuales tres son nuevos registros para el intermareal rocoso de Guerrero: las familias mejor representadas en riqueza de especies fueron Ischnochitonidae y Arcidae, y en abundancia Chitonidae y Chamidae; la densidad fue de 31,60 ind m-2. Polyplacophora presentó 42,9% de especies dominantes y Bivalvia 19,0%. Chama corallina presentó la mayor talla en longitud y Chama sordida en ancho. La riqueza de especies de moluscos registrada se considera alta y corresponde a lo esperado para un sustrato rocoso en zona tropical. Polyplacophora presentó alta composición de especies, que podría ser resultado de la adaptación de los organismos a las condiciones dinámicas del intermareal rocoso. Los nuevos registros indican la importancia de los inventarios de especies y también del conocimiento de las poblaciones y comunidades de la fauna marina de Guerrero.The State of Guerrero, Mexico, has priority marine areas for conservation of its biodiversity, but information concerning diversity of mollusks is limited, so it is convenient to carry out species inventories, studies of populations and community ecology. This study was conducted in the intertidal rocky beach Tlacopanocha. The objectives were to determine richness, density and species diversity, analyze the species composition and size structure. The sampling area was 10 m² and the sampling unit was 1 m². 35 species were identified of which three are new records for the intertidal rocky shores of the State of Guerrero. The best represented families in species richness were Ischnochitonidae and Arcidae, and Chamidae, and Chitonidae in abundance; species density was 31.60 ind m-2. Polyplacophora showed 42.9% of dominant species and Bivalvia 19.0%. Chama corallina showed the greatest length and Chama sordida the greatest width. Species richness of mollusks recorded is considered high and in agreement with tropical rocky substrates. Polyplacophora showed high species composition, which could be the result of adaptation of organisms to the dynamic conditions of the rocky intertidal. New records indicate the importance of species inventories and knowledge of the populations and community's dynamics of the Guerrero marine fauna.

  7. [Intestinal microbial biocenosis in patients with systemic lupus erythematosus treated with prednisolone].

    Science.gov (United States)

    Gul'neva, M Iu; Shilkina, N P

    2009-01-01

    This study of intestinal microflora included 60 patients with systemic lupus erythematosus (LE) and 30 ones with cardiologic problems (controls). LE was diagnosed using criteria of American Rheumatologic Association (1982). Activity of the disease was estimated based on the ECLAM index. Nineteen patients with grade II LE were given with prednisolone (15 mg/day) and 15 ones did not receive this treatment. The qualitative and quantitative composition of intestinal microflora was evaluated by bacteriological methods. Results of the study were analysed with the use of STATISTICA 6.0 software system (StatSoft). Prednisolone was shown to alter the qualitative composition of microflora and the structure of symbiotic interactions between different organisms. Specifically, colonic flora contained more enterococci and organisms possessed of hemolytic activity whereas quantitative composition remained practically unchanged. Enterococci and colibacilli with modified enzymatic activity became predominant forms. It is concluded that prednisolone therapy affects colonic microbiocenosis in patients with LE favouring the development of enterococci, atypical colibacilli, and organisms with hemolytic activity. PMID:19670716

  8. Populational dynamics of Stomoxys calcitrans (Linneaus) (Diptera: Muscidae) in three biocenosis, Minas Gerais, Brazil.

    Science.gov (United States)

    Rodríguez-Batista, Z; Leite, R C; Oliveira, P R; Lopes, C M L; Borges, L M F

    2005-06-30

    Populational flux of the adult phase of Stomoxys calcitrans was observed in the municipal district of Pedro Leopoldo, Minas Gerais, Brazil. Three biocenoses were selected for the study: stable agrobiocenosis, pastural agrobiocenosis and eubiocenosis. The occurrence and the populational flux of the insects, using the Magoon trap for their capture, were established. For each trap located in different biocenoses, a crossbred calf (Bos taurusxBos indicus) approximately 6-month-old was used as "live bait," exposed weekly for 48h in the traps. Of the three agrobiocenoses studied, the stable agrobiocenosis contributed the greatest number of specimens of. S. calcitrans captured, corresponding to 96.9% of the total flies of this species collected. S. calcitrans shows seasonal behavior for approximately 6 months (spring and summer being the rainiest months of the year). The population peaked during the months of November and December. During the months of July and August, there was no capture of flies. PMID:15908125

  9. Characterization of the cyanobacterial biocenosis of a freshwater reservoir in Italy

    Czech Academy of Sciences Publication Activity Database

    Mugnai, M. A.; Turicchia, S.; Margheri, M. C.; Sili, C.; Gugger, M.; Tedioli, G.; Komárek, Jiří; Ventura, S.

    2003-01-01

    Roč. 148, č. 109 (2003), s. 403-419. ISSN 0342-1120. [Symposium of the International Association for Cyanophyte Research /15./. Barcelona, 03.09.2001-07.09.2001] R&D Projects: GA AV ČR KSK6005114 Keywords : freshwater reservoir * cyanobacterial diversity * morphology Subject RIV: EF - Botanics

  10. Differences in ecological impacts of systemic insecticides with different physicochemical properties on biocenosis of experimental paddy fields.

    Science.gov (United States)

    Hayasaka, Daisuke; Korenaga, Tomoko; Sánchez-Bayo, Francisco; Goka, Koichi

    2012-01-01

    The environmental risks of pesticides are typically determined by laboratory single-species tests based on OECD test guidelines, even if biodiversity should also be taken into consideration. To evaluate how realistic these assessments are, ecological changes caused by the systemic insecticides imidacloprid and fipronil, which have different physicochemical properties, when applied at recommended commercial rates on rice fields were monitored using experimental paddy mesocosms. A total of 178 species were observed. There were no significant differences in abundance of crop arthropods among the experimental paddies. However, zooplankton, benthic and neuston communities in imidacloprid-treated field had significantly less abundance of species than control and fipronil fields. Significant differences in abundance of nekton community were also found between both insecticide-treated paddies and control. Influences on the growth of medaka fish were also found in both adults and their fries. Both Principal Response Curve analysis (PRC) and Detrended Correspondence Analysis (DCA) showed the time series variations in community structure among treatments, in particular for imidacloprid during the middle stage of the experimental period. These results show the ecological effect-concentrations (LOEC ~ 1 μg/l) of these insecticides in mesocosms, especially imidacloprid, are clearly different from their laboratory tests. We suggest that differences in the duration of the recovery process among groups of species are due to different physicochemical properties of the insecticides. Therefore, realistic prediction and assessment of pesticide effects at the community level should consider not only the sensitivity traits and interaction among species but also the differences in physicochemical characteristics of each pesticide. PMID:21877228

  11. [THE EFFECT OF DURATION OF ORTHODONTIC TREATMENT ON MICRO-BIOCENOSIS OF BACK OF MUCOUS MEMBRANE OF TONGUE].

    Science.gov (United States)

    Gavrilova, O A; Chervinets, I M; Chervinets, Yu V; Matlaeva, A S; Troshin, A V; Mironov A Yu

    2015-06-01

    The article presents analysis of results of studying spectrum, rate of occurrence and amount of microorganisms on mucous membrane of back of tongue before orthodontic treatment of dentoalveolar anomalies and deformations and on different stages of fixation. It is established that in all patients the dominant microorganisms of analyzed biotope were bacteria of genus of Streptococcus and Peptostreptococcus released in number of 5-6 Ig CFU/cm2. The gender and quantitative characteristics of opportunistic pathogenic microorganisms varied during all year of monitoring. However, their prevalence and number either returned to initial indicators or had no exceeding over allowed values. The results testify necessity of development of standards of follow-up of orthodontic patients over whole period of treatment with purpose of implementation of adjusting measures preventing development of complications. PMID:26466456

  12. The concept of biotope in marine ecology and coastal management.

    Science.gov (United States)

    Olenin, Sergej; Ducrotoy, Jean-Paul

    2006-01-01

    The term "biotope" was introduced by a German scientist, Dahl in 1908 as an addition to the concept of "biocenosis" earlier formulated by Möbius (1877). Initially it determined the physical-chemical conditions of existence of a biocenosis ("the biotope of a biocenosis"). Further, both biotope and biocenosis were respectively considered as abiotic and biotic parts of an ecosystem. This notion ("ecosystem = biotope + biocenosis") became accepted in German, French, Russian and other European "continental" ecological literature. The new interpretation of the term ("biotope = habitat + community") appeared in the United Kingdom in the early 1990s while classifying "marine habitats" of the coastal zone. Since then, this meaning was also used in international European environmental documents. This paper examines the evolution of the biotope notion. It is concluded that the contemporary concept is robust and may be used not only for the classification and mapping but also for functional marine ecology and coastal zone management. PMID:16600815

  13. 男性患者人型支原体液体培养呈红浑色者的病原体检测%The Biocenosis Study of the Male Patients Whoes M.hominis Fluid Culture Was Red But Turbid

    Institute of Scientific and Technical Information of China (English)

    傅琳玲; 曹双林; 符梅; 邵萍

    2007-01-01

    目的 寻找部分有尿道不适感、常规性病检查仅人型支原体培养液颜色呈红色但外观浑浊有沉淀物、经正规抗人型支原体治疗病情无好转的男性患者的致病原.方法 取患者尿道分泌物进行人型支原体固体培养和细菌培养,培养液沉渣进行革兰染色后油镜下观察.结果 患者主要为细菌生长,仅少数患者仍存在人型支原体感染.结论 对患者进行病原体检测,从而进行针对性治疗.

  14. ROLE OF BIOLOGICAL PROCESSES IN TECHNOLOGY OF GROUND WATER TREATMENT

    OpenAIRE

    Yu. P. Sedluho; M. I. Lemesh

    2015-01-01

    Technological peculiar features of biocenosis development in water treatment facilities and a role of biological processes in the technology of ground water treatment are considered in the paper. The paper provides main factors that influence on biological process development.

  15. [Evaluation of soil microbial communities in two types of Siberian forest ecosystems].

    Science.gov (United States)

    Grodnitskaia, I D; Sorokin, N D

    2007-01-01

    Microbial respiration and biomass were evaluated in soils of the Ermak Tree Nursery and Pogorel'skii Forest under different coniferous species. The degree of disturbance of each biocenosis was determined from the metabolic coefficient (qCO2). The microbial investigation demonstrated a lower resistance to ecological factors of the tree nursery biocenosis as compared to those of the Pogorel'skii Forest. PMID:19768961

  16. Abnormal changes in the density of thermal neutron flux in biocenoses near the earth surface.

    Science.gov (United States)

    Plotnikova, N V; Smirnov, A N; Kolesnikov, M V; Semenov, D S; Frolov, V A; Lapshin, V B; Syroeshkin, A V

    2007-04-01

    We revealed an increase in the density of thermal neutron flux in forest biocenoses, which was not associated with astrogeophysical events. The maximum spike of this parameter in the biocenosis reached 10,000 n/(sec x m2). Diurnal pattern of the density of thermal neutron flux depended only on the type of biocenosis. The effects of biomodulation of corpuscular radiation for balneology are discussed. PMID:18214289

  17. Effect of microflora of bed waters of the Arlan oil field on nonionogenic surfactant OP-10

    Energy Technology Data Exchange (ETDEWEB)

    Gvozdyak, P.I.; Khazipov, R.Kh.; Kravchuk, V.N.; Podorvan, N.I.; Udod, V.M.

    1983-01-01

    A study was made of the possibility of destruction of the nonionogenic surfactant (NS) OP-10 under the influence of microflora of bed waters of the Arlan field. It was established that in the studied water there is biocenosis of the microorganisms promoting destruction of NS OP-10 with initial concentration of 500 mg/l. The adapted biocenosis of the microorganisms of bed waters to OP-10 in anaerobic conditions destroys the compound by 79-85% in 6 days.

  18. INVESTIGATIONS ON SEWAGE TREATMENT PROCESS USING COMBINED BIO-OXIDIZERS

    Directory of Open Access Journals (Sweden)

    V. N. Jaromsky

    2010-01-01

    Full Text Available The paper presents results of investigations on process of aerobic waste water treatment with combined bio-oxidizers at milk processing enterprises. It has been shown that attached biocenosis, free-floating biocenosis and also bio-module rotation frequency  have exerted a significant influence on the process of an aerobic sewage treatment. It has been established that combined bio-oxidizers can be used for cleaning high concentrated waste water at the enterprises of food industry.

  19. [Study of nasal and tonsillar mucosal microbiocenoses as one of the health indices].

    Science.gov (United States)

    Khusnutdinova, L M; Usviatsov, B Ia; Soboleva, Iu V; Bukharin, O V

    2009-01-01

    The qualitative and quantitative compositions of nasal and tonsillar mucosal biocenoses were studied in healthy individuals and patients with chronic tonsillitis as an ecological criterion for assessing the stability of biocenosis and human health. The qualitative composition of tonsillar mucosal biocenosis turned out to be steady-state both in health and disease. The human nasal mucosa showed itself as an indicator system. Staphylococcal strains with a high persistent potential and polyantibiotic resistance, which may cause an exacerbation of the inflammatory process on translocation to the tonsillar mucosa, were selected on the nasal mucosa of patients with chronic tonsillitis. PMID:19799231

  20. Features intensification of treatment facilities with significant seasonal variations in loads

    Directory of Open Access Journals (Sweden)

    Olga Chjy

    2015-05-01

    Full Text Available The article is devoted to the method of intensification of wastewater treatment in a period of great seasonal fluctuations performance based on the stabilization of the operating status of the activated sludge, increasing the reduction potential and stability of biocenosis as a whole by means of making enzyme and vitamin fertilizing during the month before the expected onset of the season.

  1. [Prognostic factors of efficacy of eradication therapy in patients with duodenal ulcer].

    Science.gov (United States)

    Kozlova, I V; Eliseev, Iu Iu; Pakhomova, A L; Khan, Sadzhad Akhmad

    2005-01-01

    The aim of the study was to determine microorganism-associated predictors of efficacy of eradication therapy in patients with Helicobacter pylori-associated duodenal ulcer. The subjects were 129 such patients. Clinical, endoscopic, microbiological, and immunological examination revealed differences in the initial immune status, the structure of gastroduodenal zone mucosa, and large bowel biocenosis, which predict efficacy of eradication therapy. PMID:16117427

  2. Principles of biotechnological treatment of industrial wastes

    Energy Technology Data Exchange (ETDEWEB)

    Roig, M.G.; Martin Rodriguez, M.J.M.; Cachaza, J.M. (Univ. de Salamanca, Salamanca (Spain). Dept. de Quimica Fisica); Mendoza Sanchez, L. (C/Sol Oriente, Salamanca (Spain). Estudios y Proyectos); Kennedy, J.F. (Univ. of Birmingham, Birmingham (United Kingdom). Research Lab. for the Chemistry of Bioactive Carbohydrates and Proteins)

    1993-07-01

    This review includes current information on biodegradation processes of pollutants, digestor biocenosis and bioadditives, sludge production, measurement of pollution, and advances regarding biotechnological treatment of a series of specific industrial effluents. It was foreseen in 1980 that biotechnology would foster the creation of new industries with low energy requirements. This is because the growth of microorganisms provides a renewable source of energy.

  3. Geotrichum capitatum, a new isolate degrading phenol

    Directory of Open Access Journals (Sweden)

    Anna Grabińska-Łoniewska

    2014-08-01

    Full Text Available Geotrichum capitatum strain GID2, highly active in degradation of phenol was isolated from the biocenosis of rotating discs reactor treating petrochemical wastes. It was also able to utilize ethanol, glycerol, acetate, citrate and p-, m-cresoles as sole C-sources.

  4. Mathematical modeling of biogenous sulfate reduction in flooded carbonate petroleum reservoirs

    Energy Technology Data Exchange (ETDEWEB)

    Ametov, A.M.

    1981-07-01

    A mathematical model of the activity of biocenosis of hydrocarbon oxidizing and sulfate reducing bacteria in flooded carbonate petroleum reservoirs, which leads to hydrogen sulfide generation which makes the working of deposits difficult, is investigated. A method of suppressing the bacterial processes in the bottom hole zones of injection wells is proposed. It presupposes that no viable bacteria reach the stratum.

  5. ROLE OF BIOLOGICAL PROCESSES IN TECHNOLOGY OF GROUND WATER TREATMENT

    Directory of Open Access Journals (Sweden)

    Yu. P. Sedluho

    2008-01-01

    Full Text Available Technological peculiar features of biocenosis development in water treatment facilities and a role of biological processes in the technology of ground water treatment are considered in the paper. The paper provides main factors that influence on biological process development.

  6. Question of using microbiocenoses for increasing the oil production of a stratum

    Energy Technology Data Exchange (ETDEWEB)

    Gareyshina, A.Z.; Bikmukhametova, A.V.; Kozyuro, V.I.; Kuznetsova, T.A.; Yayus, L.I.

    1979-01-01

    Laboratory tests showed that artificial biocenosis from accumulating cultures of hydrogen, propanooxylating and gas forming bacteria do not stimulate intensive gas formation in oils of the paraffin-naphthene type and suppress the natural microflora which is present in natural oil.

  7. [Structural variability of the lithorheophile macrobenthos communities].

    Science.gov (United States)

    Chertoprud, M V

    2007-01-01

    The relationship between the abundance of taxa and life forms of lithorheophile macrobenthos and its variability were studied based on 200 quantitative samples from six territories of the Palaearctic (Moscow province, northwestern Caucasus, eastern Carpathians, northern Karelia, South Urals, and Altai mountains). The set of taxa predominant in the communities and their ecology are described. It is found that community structure varies strongly, depending on the characteristics of each region, on the size of the watercourse, and on the season. Six types of biocenoses are recognized by means of the Braun-Blanquet method, each characterized by its peculiar set of predominant life forms and families rather similar in different territories. The differences between these types are related to the size and the hydrological conditions of the watercourse. Biocenosis 1 is typical to smal brooks (up to 0.01-0.1 m3/s), characterised by the predominance of detritophagous animals non-specific to the type of food (Gammarus, Nemoura, Limnephilidae). In biocenosis 2a (large brooks with water flow 0.03-0.3 m3/s and velocity 0.1-0.3 m/s), almost immobile shell scrapers (Ancylus, Silo, Agapetes, Glossosoma) are predominant. Biocenosis 2b (large brooks with velocity 0.3-0.5 m/s) have a more or less balanced set of fundamental lithorheophile life forms. Biocenosis 2c (large mountain brooks with velocity 0.5-1 m/s) is characterised by specialized scrapers of the rapids (Epeorus and Diomesa) and filterers (Simuliidae). In biocenosis 3 (small rivers), sedentary filterers (Hydropsychidae, Simulliidae) are predominant; scrapers also play a significant role. Biocenosis 4 (rivers with water flow more than 3 m3/s, thick incrustations, and silted stones on the bottom) has predominant filterers (Hydropsychidae) and vermiform algophagous animals inside the incrustations (Orthocladius, Psychomyia). Significant variability in community structure unrelated to the environmental factors is revealed

  8. Comparison of waste water treatment between completely mixed and fluidised bed reactor; development and structure of biomass

    Energy Technology Data Exchange (ETDEWEB)

    Toman, M.; Mejac, B.

    1988-08-01

    The aerobic biological treatment of waste water from production of semisynthetic antibiotics in a completely mixed reactor and in a fluidised bed reactor was studied. The formation and development of new biomass on the sand of a fluidised bed was observed, so that differences in the structure of organisms of the concomitant biocenosis could be detected. In a fluidised bed reactor the same quality of treatednwater was gained on account of a 4-5 times higher volumetric and hydraulic loading as it was the case with a conventional activated sludge plant. The biocenosis of the fluidised bed was abundant in individua and species. The biofilm of the sand depended on substrate degradation rate as well as on rubbing among the sand particles. An optimal biofilm developed on the sand of a fluidised bed reactor 10 to 15 days after the experiment had began, and that condition remained unchanged as the experiment continued.

  9. [THE MICRO-ECOLOGY OF DIGESTIVE TRACT AS AN INDICATOR OF HUMAN HEALTH CONDITIONS].

    Science.gov (United States)

    Samoukina, A M; Mikhailova, E S; Chervinets, V M; Mironov A Yu; Alekseeva, Yu A

    2015-06-01

    The study was carried out to analyze qualitative and quantitative parameters of oral fluid and feces in 74 healthy individuals of different age groups. In most of the cases, alterations of micro-ecology are established characterizing by decreasing of amount of indigenous micro-flora and increasing of number of opportunistic pathogenic microorganisms of genera of Staphylococcus, Bacillus, Candida. The degree of evidence of these alterations reliably increases with age. It is established that microbiota, initial and terminal biotopes of digestive tract are closely interrelated and have number of common characteristics depending on age, hormonal and immune status and reflect conditions of micro-biocenosis of digestive tract in general. The character and degree of evidence of alterations of micro-biocenosis can be an effective diagnostic criterion for complex evaluation of human health conditions with following formation of risk groups in need of particular volume of correction activities. PMID:26466455

  10. Bacteria, fungi and arthropod pests collected on modern human mummies

    Directory of Open Access Journals (Sweden)

    F. Palla

    2011-08-01

    Full Text Available A survey of opportunistic biocenosis (macro and micro organisms associated with a rest of human mummy samples was carried out to characterise the biocenosis and to detect the potential of biodeteriogens. The rests of the human modern mummies come from a hypogeic site. Since mummies are relevant from a historic-artistic-scientific point of view, an aspect of this study was the identification and characterization of the biological systems related with biodeterioration of organic matter. In a first step, different sampling methods, according to the taxa, were applied. Technological procedures were combined in order to have an interdisciplinary approach to the conservation actions for testing future restoration protocols. Specimens were collected, identified and characterized by Microscopy (light, SEM, CLSM and molecular analyses (DNA extraction, in vitro target sequence amplification, sequencing, sequence analysis. The results highlight a rather complex biocenonsis consisting of fungi, cyanobacteria, several insects and other arthropods.

  11. New Trends and Perspectives in the Evolution of Neurotransmitters in Microbial, Plant, and Animal Cells.

    Science.gov (United States)

    Roshchina, Victoria V

    2016-01-01

    The evolutionary perspective on the universal roles of compounds known as neurotransmitters may help in the analysis of relations between all organisms in biocenosis-from microorganisms to plant and animals. This phenomenon, significant for chemosignaling and cellular endocrinology, has been important in human health and the ability to cause disease or immunity, because the "living environment" influences every organism in a biocenosis relationship (microorganism-microorganism, microorganism-plant, microorganism-animal, plant-animal, plant-plant and animal-animal). Non-nervous functions of neurotransmitters (rather "biomediators" on a cellular level) are considered in this review and ample consideration is given to similarities and differences that unite, as well as distinguish, taxonomical kingdoms. PMID:26589213

  12. MONITORING OF HYDROCHEMICAL INDICES AND STATE OF PHYTOCENOSES IN LITTORAL AQUATORIES OF TILIGUL ESTUARY

    Directory of Open Access Journals (Sweden)

    Bayraktar V. N.

    2012-06-01

    Full Text Available The biochemical research of coastal waters, littoral and benthic peloids, biochemical parameters of different species of macrophytes were performed in area of channel connecting the Tiligul estuary and the Black Sea. The research was conducted in various seasons. The concentration of macro - and microelements had changed in different seasons of the year. The diversity of macrophyte species was seasonally varied. It was proved that the anthropogenic load is the primary negative factor for the biocenosis.

  13. [Tendencies of nematodes communities to recover after soil cover degradation].

    Science.gov (United States)

    Gruzdeva, L I; Sushchuk, A A

    2010-01-01

    The way nematodes form communities on a new substrate after complete soil and plant cover degradation is studied on a model of industrial dumping. It is revealed that recovery of soil cover after degradation begins with invasion of mainly the upper soil horizon by nematodes. At the early stages, species that are resistant to unfavorable environmental conditions dominate (bacteriophages), next the abundances of carnivores and nematodes that are connected with plants increase, which indicates the process of biocenosis regeneration. PMID:21275095

  14. Production of molecular hydrogen under the action of microflora from oil-bearing strata on oil

    Energy Technology Data Exchange (ETDEWEB)

    Nazina, T.N.

    1981-01-01

    Methane and carbon dioxide were produced during the growth of a methane forming bacterial biocenosis from oil-bearing strata in the cultural broth of hydrocarbon oxidizing microorganisms grown in a medium with methane-naphthene oil. Molecular hydrogen can be produced by fermenting microorganisms isolated from stratal water in the course of their growth in the cultural broth of hydrocarbon oxidizing bacteria cultivated in media with methane oil, methane-naphthene oil and naphthene-aromatic oil.

  15. [Species composition and distribution of foraminifers in the Deryugin Basin (Sea of Okhotsk)].

    Science.gov (United States)

    Khusid, T A; Domanov, M M; Svinininnikov, A M

    2006-01-01

    Analysis of the composition and quantitative distribution of foraminifers in bathyal sediments collected at 14 stations in the Deryugin Basin and at 11 stations in other regions of the Sea of Okhotsk, Sea of Japan, and North Pacific demonstrated specific foraminifer complex in the basin at depths from 1650 to 1800 m associated with cold barite/methane seeps. Oligomixed biocenosis with prevailing agglutinated foraminifers and Saccorhiza ramosa as the dominant was shown to develop in these zones. PMID:16634440

  16. Foaming control in a medium activated sludge plant; Controllo dei fenomeni di formazione di schiume (foaming) in un depuratore a fanghi attivi di medie dimensioni

    Energy Technology Data Exchange (ETDEWEB)

    Simioli, Paola; Serravalli, Gianluca; Janni, Laura [Ferrara, Univ. (Italy). Ist. di Igiene e Medicina Preventiva; Gregorio, Pasquale [A.CO.SE.A., Ferrara (Italy)

    1997-02-01

    The present work identifies the cause of the appearance of the foaming in the aeration basins and on the secondary clarifiers through the comparative study of activated sludge biocenosis and of the physical chemical parameters of an activated sludge plant. The use of aerobic selector and the increase of dissolved oxygen concentration results in a remarkable reducing of the filamentous micro-organisms growth (prevalently Microthrix parvicella) and foam phenomena.

  17. Formation of molecular hydrogen by the action of stratal microflora on oil

    Energy Technology Data Exchange (ETDEWEB)

    Nazina, T.N.

    1981-01-01

    Methane and carbon dioxide were produced during the growth of a methane forming bacterial biocenosis from oil-bearing strata in the cultural broth of hydrocarbon oxidizing microorganisms grown in a medium with methane-naphthene oil. Molecular hydrogen can be produced by fermenting microorganisms isolated from stratal water in the course of their growth in the cultural broth of hydrocarbon oxidizing bacteria cultivated in media with methane oil, methane-naphthene oil and naphthene-aromatic oil.

  18. Bizkaiko (Euskal Herria megaforbia goimenditar mailako Karabido interesgarriak (Coleoptera : Carabidae, Pterostichinae, Nebrinae, Trechinae

    Directory of Open Access Journals (Sweden)

    HIRIBARNEGARAI, F.

    2010-01-01

    Full Text Available Los lugares donde aparece la “megaforbia” en el macizo del Gorbea (Bizkaia, junto con ciertas subfamilias de carábidos de la misma, reproducen una biocenosis que recuerda mucho a la existente en los pisos subalpino y altimontano de los Pirineos y la Cordillera Cantábrica. Se cita por primera vez en Bizkaia a Pterostichus dufouri (Dejean 1828.

  19. Characterization of Salmonella Occurring at High Prevalence in a Population of the Land Iguana Conolophus subcristatus in Galapagos Islands, Ecuador

    DEFF Research Database (Denmark)

    Franco, Alessia; Hendriksen, Rene S.; Lorenzetti, Serena;

    2011-01-01

    The aim of the study was to elucidate the association between the zoonotic pathogen Salmonella and a population of land iguana, Colonophus subcristatus, endemic to Galapagos Islands in Ecuador. We assessed the presence of Salmonella subspecies and serovars and estimated the prevalence of the path...... possible exposure of the iguanas and the biocenosis to direct or indirect environmental factors influenced by the use of antimicrobials in agriculture, in human medicine or in veterinary medicine....

  20. Mathematical modeling of biogenic sulfate reduction in floodable carbonate oil collectors

    Energy Technology Data Exchange (ETDEWEB)

    Ametov, A.M.

    1981-01-01

    A mathematical model for the activity of biocenosis of hydrocarbon-oxidizing and sulfate reducing bacteria is studied in floodable carbonate oil collectors. It results in the formation of hydrogen sulfide. This complicates working the fields. A method is proposed for suppressing bacterial processes in the near-face zone of the injection wells on the condition that the viable bacteria cease to enter the bed.

  1. Influence du statut trophique de cours d`eau sur les fonctionnalités microbiennes des biofilms(épipsammon et épilithon)

    OpenAIRE

    Madigou, E.

    2005-01-01

    On top of « natural » characteristics that dominate in a given drainage basin, the solute and composition of rivers are influenced by anthropogenic factors due to human activities. These factors may lead to the alteration of the biocenosis physiological characteristics. Periphytic communities, by their essential function within biogeochemical cycles, may represent good bioindicator of the ecological quality of a given river. The present study has been done on three rivers of different trophic...

  2. Multi-Level Approach of the Ecotoxicological Impact of a Combined Sewer Overflow on a Peri-Urban Stream

    OpenAIRE

    2012-01-01

    In periurban zones, urban wet weather discharges have been recognized as the most significant vector of pollution in aquatic environments. The discharge of this water without treatment into the aquatic environment could present an ecotoxicological risk for biocenosis. The aim of the INVASION project is to assess the potential ecotoxicological im-pact of a combined sewer overflow (CSO) on a peri-urban stream. A comparative study between upstream and down-stream areas of the CSO allowed observi...

  3. Microbial community structure of activated sludge in treatment plants with different wastewater compositions

    OpenAIRE

    Shchegolkova, Nataliya M.; George S Krasnov; Belova, Anastasia A.; Alexey A Dmitriev; Sergey eKharitonov; Klimina, Kseniya M.; Nataliya V. Melnikova; Anna V Kudryavtseva

    2016-01-01

    Activated sludge (AS) plays a crucial role in the treatment of domestic and industrial wastewater. AS is a biocenosis of microorganisms capable of degrading various pollutants, including organic compounds, toxicants and xenobiotics. We performed 16S rRNA gene sequencing of AS and incoming sewage in three wastewater treatment plants (WWTPs) responsible for processing sewage with different origins: municipal wastewater, slaughterhouse wastewater, and refinery sewage. In contrast to incoming was...

  4. Microbial Community Structure of Activated Sludge in Treatment Plants with Different Wastewater Compositions

    OpenAIRE

    Shchegolkova, Nataliya M.; George S Krasnov; Belova, Anastasia A.; Alexey A Dmitriev; Kharitonov, Sergey L.; Klimina, Kseniya M.; Nataliya V. Melnikova; Anna V Kudryavtseva

    2016-01-01

    Activated sludge (AS) plays a crucial role in the treatment of domestic and industrial wastewater. AS is a biocenosis of microorganisms capable of degrading various pollutants, including organic compounds, toxicants, and xenobiotics. We performed 16S rRNA gene sequencing of AS and incoming sewage in three wastewater treatment plants (WWTPs) responsible for processing sewage with different origins: municipal wastewater, slaughterhouse wastewater, and refinery sewage. In contrast to incoming wa...

  5. Traits fonctionnels, variabilité environnementale et bioindication : les communautés piscicoles des cours d’eau européens

    OpenAIRE

    Logez, M.

    2010-01-01

    The Water Framework Directive (WFD) establishes a framework for protecting and managing the different waterbodies: surface, transitory, coastal and underground waters. The WFD specifies that the different biocenosis components (diatom, macrophyte, benthic macroinvertebrate, and fish) must be taken into account in assessing the condition of waterbodies, a major innovation compared to past policies. The WFD recommends evaluating the ecological status of the waterbodies by comparing the obser...

  6. Remobilisation of uranium from contaminated freshwater sediments by bioturbation

    OpenAIRE

    Lagauzère, S.; M. Motelica-Heino; E. Viollier; Stora, G.; Bonzom, J.M.

    2014-01-01

    Previous studies have demonstrated that benthic macro-invertebrate bioturbation can influence the remobilization of uranium initially associated with freshwater sediments resulting in a high release of this pollutant through the overlying water column. Giving the potential negative effects on aquatic biocenosis and the global ecological risk, it appeared crucial to improve our current knowledge concerning the uranium biogeochemical behaviour in sediments. The present study aimed to ass...

  7. Biologically Qualified Environment, Ecologically Evaluated Conditions

    Directory of Open Access Journals (Sweden)

    Judit Németh-Katona

    2008-06-01

    Full Text Available The biological quality of an environment is defined as the condition reflecting thequantitative representation of living organisms in a given space and time. My lecturepresents how to qualify the environment of a biocenosis synbiologically, in other words, onthe supraindividual level. In this case, qualifying the environment in practice means thatboth the quantitative and qualitative composition of the biocenosis, and also the factorsresponsible for their distribution in space and time are considered and evaluated ascharacteristics. The ultimate goal of examining conditions is to evaluate, in other words, todetermine relevancy and significance in a given biocenosis. We are facing the problem thatin Hungary at present the biological survey methods are not standardized, and they arehighly varied. An environmental information system however cannot function beforeappropriate methods of biological survey are created. The lack of such methods wouldresult that one of the three major, organically complementary sources of information (i.e.the abiotic sphere, the biosphere, and human society is completely missing. From adifferent aspect, ecological survey studies are different from the rest of environmentalsurvey studies, inasmuch as they study living organisms and their communities, thusinevitably utilizing the results of other environmental survey studies that evaluate differentcomponents. This indicates that the ecological section of all environmental survey studies isvitally important due to its complexity, and should be considered primarily decisive.

  8. Scientific Rationale and Algorithmization of Sochi Resort Natural and Preformed Physical Therapeutic Factors in the Course of Combined Treatment of Patients with Chronic Vulvovaginitis

    Directory of Open Access Journals (Sweden)

    Kirill V. Gordon

    2012-11-01

    Full Text Available Chronic vulvovaginitis is the most wide-spread gynecological pathology among women of childbearing age. The chief causations of nonspecific vulvovaginitis are reduction in oestrogen, disturbances in vaginal acidity, intestine dysbacteriosis, local and general immune disturbances. The growth of vagina opportunistic pathogenic flora is closely connected with the increase of bacterial slimes on vagina mucus. Sulphur balneotherapy in the form of general baths and vaginal irrigations, mechanical physiotherapy promotes disruption of bacterial slimes, oestrogen elevation, immune reconstruction and vaginal biocenosis, which has positive effect on patients’ fertility level, frequency of obstetrical and perinatal pathologies.

  9. [Characteristics of associated microflora of ejaculate from males with gonococcal infections].

    Science.gov (United States)

    Voronina, L G; Mikhaĭlova, E A; Perunova, N B; Kuznetsova, E K

    2006-01-01

    Species composition and biological properties of aerobic and anaerobic microflora from an ejaculate from males with acute and chronic gonococcal infection and from healthy men were studied. Patients with gonococcal infection were shown to present quantitative and qualitative changes in microbiocenosis of the reproductive tract. Patients with acute gonorrhea displayed decreased species diversity in contrast to healthy persons, while different species of associated with gonococci microbes with high persistent potential, were cultured in patients suffering from a chronic form of infection. The role of revealed pathological biocenosis patterns in developing chronic infection as well as postgonorrheal gonococci-free urethritis is discussed. PMID:16941881

  10. BODY MEASUREMENT OF WOODCOCK (SCOLOPAX RUSTICOLA L. IN CENTRAL CROATIA

    Directory of Open Access Journals (Sweden)

    Nikica Šprem

    2010-10-01

    Full Text Available Woodcock (Scolopax rusticola, Linnaeus 1758 is one of the most prevailing types of the Scalopax species, and presents the important species in the entire biocenosis. Although today the woodcock population is considered stable worldwide, the newest research in biology and concrete guidelines in hunting management are necessary for the future. The reason to start this research was simply this unsufficiently researched population of woodcock which has its habitat in our area. According to the data available from the literature morphological values gathered match.

  11. [Morphology and structural peculiarities of Basidiomycetes pathogens].

    Science.gov (United States)

    Boĭko, O A; Shevchenko, T P; Boĭko, A A

    2013-01-01

    The materials of studies of morphology and structural peculiarities of viruses, fungi and bacteria, which affect Basidiomycetes under biotechnology process and nature biocenosis conditions are given. The analysis of infection development in button mushroom (Agaricus bisporus) (J.Lge) Imbach and in oyster mushroom (Pleurotus ostreatus Kumm.), which served as model objects in the experiments of various levels of complexity has been carried out. Other kinds of edible and medicinal mushrooms, which were a source of biochemical fractions to form biologicals were investigated. PMID:23866587

  12. COMPLEX COMPOST AND ECOLOGICAL NICHE OF LIVING ORGANISMS IN AGROLANDSCAPES

    Directory of Open Access Journals (Sweden)

    Belyuchenko I. S.

    2014-09-01

    Full Text Available Ecological niche acts as an important evolutionary function of living organisms in the ecosystem and determines its properties, which are caused by the nutrient requirements, mobility, ability to reproduction, biochemical features, structural features limits tolerance to environmental conditions, opportunities performing certain actions in the specific biocenosis; in the process of divergence (species, their internal and external properties and abiotic characteristics features of ecological niches are changed , there are new kinds of living organisms and, of course, the formation of new ecological niches

  13. Biogenic hydrogen sulfide in the oil gas of Western Siberian fields

    Energy Technology Data Exchange (ETDEWEB)

    Yershov, V.A.; Chetverkina, V.N.; Nosova, V.S.; Shakirova, A.Kh.

    1984-01-01

    In connection with the discovery of biogenic hydrogen sulfide in the oil gas of Western Siberian fields, the quantity of hydrogen sulfide has been monitored and the dynamics of the development of the sulfate reduction processes and their features are examined. It is noted that in the absence of influences on the bacterial flora, it is necessary to eliminate hydrogen sulfide from natural gas or to use hydrogen sulfide corrosion inhibiters in order to suppress biocenosis in building gas processing plants and gas lift systems, in order to reduce equipment corrosion.

  14. Physical and hydrobiological process of heat pollution

    Energy Technology Data Exchange (ETDEWEB)

    Szolnoky, C.

    1981-01-01

    The basis for hydrobiological research on heat polluted rivers and an analysis of the effects on biocenosis. Characteristic heat pollution on the Dunay and Tiss rivers is analyzed. One way of lessening pollution is to lessen river heating. Permissable heat limitations must not only one-sidedly safeguard hydrobiological processes but also the dynamic balance of the water-heat regime of the rivers, as well as energy production, including preserving water quality. Lessening the danger from heat pollution can be accomplished only with defined technical measures (introducing oxygen, adding cooling towers) preceded by substantive study of hydrologic-hydraulic characteristics influencing water quality.

  15. Silicon multi-junctional solar cells with vertical p-n junctions: Evolution, technology, applications, and new opportunities

    Energy Technology Data Exchange (ETDEWEB)

    Simakin, V.V. [All-Russian Institute of Electrical Engineering, Moscow (Russian Federation); Strebkov, D.S.; Tyukhov, I.I. [All-Russian Institute for Electrification of Agriculture, Moscow (Russian Federation)

    2004-07-01

    This paper describes the evolution of the silicon multi-junctional solar cells with vertical p-n junctions (SCVJ) and its present status, mainly, at the base of researches carried out in Russia and the USA. The number of SCVJ produced in the whole world is quite low in comparison with the traditional SC. At the same time according to the laws of technical cenosis (as in biology - biocenosis) increasing of unification and size of SC leads to increasing economic efficiency of solar energy and, yet, diversification (developing and creating other types of SC, and other principles) leads to higher competitiveness and occupying new niches. (orig.)

  16. Effect of activated sludge in the bottom zone on biogenic sulfate reduction

    Energy Technology Data Exchange (ETDEWEB)

    Yagafarov, G.G.; Bikchentayeva, A.G.; Yagafarov, R.G.

    1981-01-01

    It is shown that sulfate destruction in the Arlansk group of fields is caused by infection of the formation by sulfate reducing bacteria in the drilling process and flooding by surface water. For the first time, the necessity is shown of considering the activated sludge formed from particles suspended in water and biocenosis of microorganisms during microbiological investigation of wells. It is suggested that biodecomposition of surfactants is possible only in the area of formation of activated sludge around the bottom of the injection well.

  17. [Radioecological biomonitoring of the radioactive waste long-term storage territories].

    Science.gov (United States)

    Sypin, V D; Pol'skiĭ, O G; Sobolev, A I; Verbov, V V; Zaĭtsev, V V; Osipov, A N

    2009-01-01

    Radionuclide release to environment is possible during long-term storage of the low and middle activity radioactive waste on specially equipped territories, which leads to radioactive background increase and to permanent radiation influence to biocenosis. For an ecological situation control in such places it is need to provide a biomonitoring using the method of complex estimation of the morphological changes on whole organism and internal organs levels (presents of tumors, teratogenic effects), the hematological indexes reflected quantifies and qualifies changes in blood, cytogenetic distribution (bone marrow polychromatic erythrocytes with micronuclei) and distributions on molecular level (alterations of DNA structure lead to increase in the DPC level). PMID:19637745

  18. Trypanosoma cruzi, causal agent of Chagas disease: The borderline between wild and domestic cycles in Venezuela.

    Directory of Open Access Journals (Sweden)

    Leidi eHerrera

    2014-11-01

    Full Text Available American trypanosomiasis or Chagas disease, caused by Trypanosoma cruzi, occurs between triatomine vectors and mammals, including man. T. cruzi has 150 Ma in America with almost 10 million of infected people today. The overlapping of its wild and domestic ecotopes is increasing. The host-parasite imbrications has been discerned by the study of infection patterns, transmissibility and transmission cycles in natural and laboratory models, through to parasitological and molecular tests. This article describes specific parasite niches, as plant biocenosis or biological corridors between domestic and wild ecotopes and helps distinguish Chagas disease risks and the borderline between wild and domestic transmission cycles, with emphasis on Venezuelan studies.

  19. [Immunity and normal pharyngeal microflora in persons living in a technogenically exposed area].

    Science.gov (United States)

    Kolenchukova, O A; Savchenko, A A

    2006-01-01

    The impact of industrial emissions of an aluminum plant of immunity and pharyngeal mucosal biocenosis were studied in healthy individuals living in the Sovetsky district. The effect of sodium fluoride in various concentrations on the activity of metabolic enzymes in the bacterial Staphylococcus epidermis cells isolated from the pharyngeal mucosa of the healthy individuals was also studied. The persons living in the Sovetsky district were found to have higher values of cellular immunity, an increase in the quantitative composition of the oral microflora being observed when its qualitative composition was worse. Various concentrations of sodium fluoride were ascertained to have a heterodirectional impact on microbial metabolism. PMID:17190044

  20. [Candida and Saccharomyces spp. fungal associations in fecal microbiocenosis of diabetes patients and healthy subjects].

    Science.gov (United States)

    Gerasimova, E V; Nesvizhskiĭ, Iu V; Maĭorova, N M; Bogdanova, E A

    2010-01-01

    The article is devoted to analysis of pathogenic and diagnostic significance of Candida and Saccharomyces co-existence in diabetic patients. These transient fungi are known to be present in fecal microbiocenosis of both healthy subjects and patients with diabetes mellitus. However, their overall occurrence is significantly increased in the disease and the structure of the biocenosis undergoes alteration. These data confirm the role of yeast-like fungi in pathogenesis of diabetes. The diagnostic value of detection of monospecific and mixed populations of Candida and Saccharomyces spp. is not very high, but their presence in feces, especially in women, may be regarded as a sign of disturbed carbohydrate metabolism. PMID:21395061

  1. Influence of physical and chemical methods of enhanced oil recovery in formation microflora and properties of crude oil

    Science.gov (United States)

    Shcherbakova, A. G.; Altunina, L. K.; Svarovskaya, L. I.; Ovsyannikova, V. S.; Filatov, D. A.; Chuikina, D. I.

    2015-10-01

    The results of the analyzes of crude oil and produced water from wells in the areas of pilot testing of new flow deflection and oil-displacing Compounds developed in the Institute of Petroleum Chemistry SB RAS. It was found that changes in the properties and Compound of the oil and water mainly occur in the redistribution of filtration flows and integration in the development of the previously unwashed areas, as well as in washing off the residual heavy oil from the reservoir rock, and in some wells - due to formation biocenosis, contributing to desorption of oil from the rock.

  2. On the way to identify microorganisms in drinking water distribution networks via DNA analysis of the gut content of freshwater isopods.

    Science.gov (United States)

    Mayer, Michael; Keller, Adrian; Szewzyk, Ulrich; Warnecke, Hans-Joachim

    2015-05-10

    Pure drinking water is the basis for a healthy society. In Germany the drinking water regulations demand for analysis of water via detection of certain microbiological parameters by cultivation only. However, not all prokaryotes can be detected by these standard methods. How to gain more and better information about the bacteria present in drinking water and its distribution systems? The biofilms in drinking water distribution systems are built by bacteria and therefore represent a valuable source of information about the species present. Unfortunately, these biofilms are badly accessible. We thus exploited the circumstance that a lot of metazoans graze the biofilms, so that the content of their guts partly reflects the respective biofilm biocenosis. Therefore, we collected omnivorous isopods, prepared their guts and examined and characterized their contents based on 16S und 18S rDNA analysis. These molecularbiological investigations provide a profound basis for the characterization of the biocenosis and thereby biologically assess the drinking water ecosystems. Combined with a thorough identification of the species and the knowledge of their habitats, this approach can provide useful indications for the assessment of drinking-water quality and the early detection of problems in the distribution system. PMID:25558805

  3. [Comparative characterization of the microflora of the upper respiratory tract mucous membranes in bronchial asthma and allergic persistent rhinitis].

    Science.gov (United States)

    Romanenko, E E; Baturo, A P; Ulisko, I N

    2005-01-01

    A total of 250 patients with diagnosed bronchial asthma (BA) were examined by microbiological methods. Among them--188 children and 62 adults. In 87 patients the microflora of nasal mucosa was studied, in 40--of pharynx only and in 123 patients--both the nasal and the pharynx. For comparative analysis earlier data obtained in 69 patients with persistent allergic rhinitis (PAR) were used. The cultures isolated from the nasal mucosa of BA patients were shown to number 18 genera and 42 species, while among those isolated from pharynx mucosa 20 genera and 40 species. Monocultures were isolated from the nasal mucosa only in 23% of the examined patients and from the pharynx mucosa--only in 1.42%. Associations with different numbers of components were isolated from nasal and pharynx mucosa (2 to 6, 2 to 8 respectively). Staphylococcus aureus was regarded as the main species of nasal biocenosis in BA and PAR, as well as pharynx biocenosis in BA. Besides, in BA other Staphylococcus species (schleiferi, caprae, capitis, hominis, etc.), reversely related to the main species, could be isolated from both mucous membranes. Similarities and differences in microflora of biocenoses in both nosological forms, confirming links between PAR and BA, are considered. PMID:15881942

  4. Relevance of micro- and blue-green algae to the degradation of xenobiotics in a model system simulating surface waters. Final report. Die Bedeutung von Mikro- und Blaualgen fuer den Abbau xenobiotischer Verbindungen in einem Oberflaechengewaesser-simulierenden Testsystem. Schlussbericht

    Energy Technology Data Exchange (ETDEWEB)

    Steber, J.

    1986-12-01

    For the purpose of a more realistic assessment of the degradability of chemicals in surface waters it was investigated whether microalgae and blue-green algae contribute significantly to the degradation in corresponding model tests. The ultimate degradation of 22 /sup 14/C-labelled xenobiotics was studied in a shake flask test by comparing conventional test conditions (bacterial inoculum, incubation in the dark) with those in the presence of an artificial biocenosis of bacteria and algae (light/dark incubation). With regard to total biodegradation (/sup 14/CO/sub 2/ + /sup 14/C-biomass), mainly comparable results were obtained. Only pentachlorophenol and aminotrismethylene- and ethylenediamine tetramethylene phosphonic acids showed an increased degradation in light-exposed and bacteria/algae-inoculated assays. More detailed investigations indicated a microalgae-independent photodegradation of PCP; moreover, algae seem to be involved only indirectly in the extensive degradation of the two phosphonic acids. It was concluded that microalgae and blue-green algae, in most cases, do not significantly contribute to the degradation potency of a surface water model system. Thus, routine degradation tests employing a bacteria/microalgae-biocenosis are not considered worthwhile. With 27 refs., 5 tabs., 4 figs.

  5. Ecological factors regulating growth of seaweeds in Arctic communities

    Directory of Open Access Journals (Sweden)

    Shoshina E. V.

    2016-03-01

    Full Text Available Features of seaweeds in the Arctic communities in connection with periodic and unperiodic influences of ecological factors have been analyzed. It has been shown that the existence of benthic algae biocenosis of the northern seas is mainly controlled by the primary periodic environmental factors acting as triggers that determine the direction of vegetative and generative processes, as well as contribute to the emergence of adaptive devices to extreme environmental conditions. Therefore, periodic exposure to environmental factors cause only structural changes in plant communities due to the elastic stability of fucus algae populations acquired as a result of the long process of adaptation to the northern seas conditions. Unperiodic primary factors also violate the ratio of the number by elimination and inhibit growth of certain algae age stages. However thanks to the stability of resistant the algae community can eventually restore its structural and functional organization

  6. [Microflora of pharyngeal mucosa in children with solid tumors].

    Science.gov (United States)

    Polishchuk, V B; Baturo, A P; Romanenko, E E; Kostinov, M P; Zaeva, G E; Mikhaĭlova, S N; Leonova, A Iu; Moiseenko, E I

    2008-01-01

    Microbiological study of pharyngeal mucosa in 43 children with solid tumors revealed that 77.2% of isolated microorganisms belonged to Gram-positive flora. It was shown that streptococci and Staphylococcus aureus were the main species. Species composition of streptococci included both pyogenic (S. pyogenes, S. agalactiae, S. dysgalactiae, S. equi) andviridans species (S. acidominimus, S. oralis and "S. milleri" group). Nocardioform actinomycetes, corynebacteria and other staphylococci were referred to additional microflora. Accidental microflora was represented by Neisseria spp., non-fermenting Gram-negative bacteria, enterobacteria and yeast-like fungi. Microbiologic study of pharyngeal mucosa biocenosis showed that monoculture was present only in 2.3% of cases; in other cases microorganisms formed both intra-genus and inter-species associations. 2-6-component associations were revealed with predominance of 3-4-component associations (37.2% and 32.6% respectively). Relationship of distribution of microorganisms belonging to main and additional microflora was revealed. PMID:19186552

  7. [Microsymbiocenosis of Codiella mollusks as a basis for symbiotic relations in the parasite-host system in opisthorchiasis].

    Science.gov (United States)

    Kataeva, L V; Karpukhina, N F; Stepanova, K B; Kolotova, O N

    2014-01-01

    The purpose of the first step of microparasitocenosis investigation was to study the microbiocenosis of the first intermediate hosts of O. felineus--prosobranch gastropods of the genus Codiella, as well as their habitats. Materials were collected in the Iryum River of the Ob-Irtysh basin. The microflora of mollusks, water, and soil from their habitats was examined. The predominant flora was Aeromonas species in the biocenosis of mollusks and Enterobacteriaceae in the microbiocenosis of the water basin and soil. Examination of the microbial communities in the mollusks and their habitats showed that the range of microbial populations of mollusks was wider in species composition as compared to the microbiocenosis of soil and water. PMID:25286543

  8. Detection of sulfonamide resistance genes via in situ PCR-FISH.

    Science.gov (United States)

    Gnida, Anna; Kunda, Katarzyna; Ziembińska, Aleksandra; Luczkiewicz, Aneta; Felis, Ewa; Surmacz-Górska, Joanna

    2014-01-01

    Due to the rising use of antibiotics and as a consequence of their concentration in the environment an increasing number of antibiotic resistant bacteria is observed. The phenomenon has a hazardous impact on human and animal life. Sulfamethoxazole is one of the sulfonamides commonly detected in surface waters and soil. The aim of the study was to detect sulfamethoxazole resistance genes in activated sludge biocenosis by use of in situ PCR and/or hybridization. So far no FISH probes for the detection of SMX resistance genes have been described in the literature. We have tested common PCR primers used for SMX resistance genes detection as FISH probes as well as a combination of in situ PCR and FISH. Despite the presence of SMX resistance genes in activated sludge confirmed via traditional PCR, the detection of the genes via microscopic visualization failed. PMID:25115110

  9. Comparison of ammonia-oxidizing bacterial community structure in membrane-assisted bioreactors using PCR-DGGE and FISH.

    Science.gov (United States)

    Ziembińska, A; Ciesielski, S; Gnida, A; Zabczyńki, S; Surmacz-Górska, J; Miksch, K

    2012-08-01

    The ammonia-oxidizing bacterial (AOB) communities in three membrane bioreactors (MBRs) were monitored for 2 months after an acclimation period in order to investigate the influence of sludge age and medium type on AOB changeability and its connection with nitrification effectiveness. One MBR with a sludge age of 4 days was fed with a synthetic medium, whereas the other two with sludge ages of 8 and 32 days were fed with landfill leachate. The research revealed that landfill leachate can be effectively treated in an MBR with a higher sludge age for longer periods of time and that this improvement in performance was correlated with an increase in AOB biodiversity. Interestingly, the medium type has a stronger influence on AOB biocenosis formation than the sludge age. PMID:22713978

  10. [Molecular mechanisms of the plague pathogenic agent interaction with invertebrates].

    Science.gov (United States)

    Kutyrev, V V; Eroshenko, G A; Popov, N V; Vidiaeva, N A; Konnov, N P

    2009-01-01

    Microbe Russian Anti-Plague Research Institute, Saratov, Russia The literature data and experimental results of the authors on the molecular basis of plague agent interaction with invertebrates are discussed. The details of the plague agent life cycle, its genome organization, and molecular genetic mechanisms of its survival in flea vector and on the nematode cuticule are discussed. The experimental data about the ability to form biofilms at abiotic and biotic surfaces in the Yersinia pestis strains of the main and non-main subspecies are presented. Mechanisms of horizontal and vertical transmission of plague agent are considered. The suggestion about participation of the new member in the complex parasitic biocenosis (nematode, vector parasite) is put forward. PMID:20050160

  11. [Microflora formation in the newborn in maternity hospitals and neonatal abnormality units].

    Science.gov (United States)

    Shilova, V P; Rozanova, S M; Kyrf, M V; Beĭkin, Ia B; Kuznetsova, L S; Turintseva, E G; Usova, O P; Chernykh, N G; Iagafarova, I S

    2007-10-01

    The basic sources of pyoseptic infection pathogens are infected and colonized neonatal infants in maternity hospitals. Microbiological monitoring revealed the specific features of biocenosis formation in the newborn in the "Mother and Baby" units, resuscitative departments (RD), intensive care units, and neonatal abnormality departments (NAD). Irrespective of the conditions of hospital stay, methicillin-resistant S. epidermis (MRSE) and Enterococcus faecium were prevalent in the neonatal microbial landscape. Colonization with the normal flora in the newborn actively treated with antibiotics is difficult in RD, at the same time there is a significant infection with the mycotic flora. Broad-spectrum beta-lactamase producing Klebsiela pneumonia strains have received wide acceptance in NAD. PMID:18154133

  12. Methane from CO₂: Influence of different CO₂ concentrations in the flush gas on the methane production in BMP tests.

    Science.gov (United States)

    Koch, Konrad; Huber, Bettina; Fernández, Yadira Bajón; Drewes, Jörg E

    2016-03-01

    The influence of carbon dioxide (CO2) in the headspace gas on the specific methane (CH4) production of blank samples with just inoculum during Biochemical Methane Potential (BMP) tests was studied. The headspace of the bottles had been flushed with 15 different ratios of CO2 and N2 prior to incubation, while they were treated otherwise identically. The results revealed that the CH4 yield increased linearly with higher ratio of CO2 in the flush gas reaching a 30% higher yield at pure CO2 relative to pure N2 headspace conditions. However, a slightly distinct lag is noticeable during the initial phase of the degradation process at high ratios of CO2, hypothesizing a reversible disturbance of the biocenosis. Further experiments and analyses need to be performed to elucidate the underlying mechanisms. PMID:26818184

  13. [Microbiocenosis of parietal mucin in the gastrointestinal tract of rats].

    Science.gov (United States)

    Vorob'ev, A A; Nesvizhskiĭ, Iu V; Bogdanova, E A; Korneev, L M

    2005-01-01

    The qualitative and quantitative composition of the microbial community in parietal mucin at different areas of the gastrointestinal tract (GIT) of rats was revealed. The pronounced variability in the quantitative and qualitative characteristics of microbiocenosis in parietal mucin of rats at different sections was revealed. The differences were most pronounced in the passage from upper to lower GIT sections, the large intestine found to be the richest biocenosis. The microbial composition of rat feces was faintly associated with the GIT parietal microbiocenosis. The individual areas of GIT mucosa were unique of their microbial characteristics and organization. This makes it possible to regard them as relatively independent biotopes and indicates that it is impossible to evaluate the microbial community by one of the colonic mucosal sifes. PMID:16438365

  14. Water pollution - phycological perspectives

    Energy Technology Data Exchange (ETDEWEB)

    Gaur, J.P.; Rai, L.C.

    1983-01-01

    Water pollution exerts a pressure of selection on algal populations. In spite of a possible adaptation, often a changed diversity and sociological structure result, from which other effects on higher levels of the nutrient chain may emanate. There are presented some biological indices for characterizing algal communities which may serve as a biological measure of pollution and self-purification, the problem of diversity being especially taken into account. Moreover, algal tests are used for representing the trophic situation and for determining the limiting nutrient, but also for determining the toxic influencing of biocenosis by hydrocarbons, too. Special attention is paid to heavy metals with regard to their synergistic action and bioaccumulation. On the other hand, the mass culture of algae is a value method of wastewater treatment and the recovery of valuable materials, and algae ponds provide an important technique for advanced wastewater purification.

  15. [Application of Bifi-forma in a complex treatment of children of the early age with non-hospital pneumonia].

    Science.gov (United States)

    Smiian, O I; Vasyl'ieva, O H

    2010-01-01

    The majority of patients with non-hospital pneumonia (NHP) on background of antibioticotherapy had changes of a microbiocenosis of their intestines. The article presents obtained results on the assessment of Bifi-forma probiotic use for 63 children aged from 1 month till 3 years. It has been shown that course of the pneumonia has been associated clinically with dyspepsic pain syndromes and with significant changes of intestine biocenosis by quantity reduction of bifido--and lactobacteria, decrease in escherichia having enzymatic activity and increase in hemolytic escherichia, yeastlike mushrooms, staphylococcus and other representatives of opportunistic flora. Application of this probiotic in a complex therapy on non-hospital pneumonia has shown high clinical and microbiological efficiency that allows recommending its administration to children of early age, patients with non-hospital pneumonia. PMID:21714291

  16. THE EFFECT OF BIOSOLIDS ON MEDICAGO SATIVA AND FESTUCA ARUNDINACEEA PLANTS PHYTOREMEDIATION PROCESS OF WASTE DUMP

    Directory of Open Access Journals (Sweden)

    B. LIXANDRU

    2013-12-01

    Full Text Available The storage of waste dump gravely deteriorates the soil’s physical, chemical and biological properties, both in the storage area and the surrounding zones. In time, serious perturbations may occur in the biocenosis from the surrounding ecosystems, through the sweeping away of the inorganic matter or through precipitations. The main problem in this case is to find the most suitable phytoremediation method for these waste dumps, by sowing plant species (grasses, in order to stop the erosion generated by wind and rain. Establishing a herbaceous “carpet” , in time, is very difficult, because the waste dump contains no organic matter, has a weak water retaining capacity and has a high bareness index. The purpose of this study was the experimentation of a method of cultivating the waste dumps using alfalfa, fescue and biosolids as fertilizers.

  17. Study on the heavy metals having effect on the water biocenoses in the backwaters at Alpar and Lakitelek

    Energy Technology Data Exchange (ETDEWEB)

    Fekete, E.

    1984-01-01

    Heavy metals which have an effect on the water biocenosis were determined monthly during 1982 in the Tisza backwaters at Alpar and Lakitelek, Hungary. Copper, cadmium, zinc, chromium, and mercury contents of the water were determined. Organic matter, ammonium, nitrate, pH, sodium and potassium content were determined jointly with the studies of the heavy metals. On the basis of water quality indexes, the water quality of Lakitelek backwater is more stable than that of the Alpar backwater. Correlations were observed between the zinc and copper content and aquatic life. The two backwaters have significant mercury content, which is due to agricultural runoff. It is striking that cadmium does not occur in significant concentrations in the backwaters. Samples originating from the bottom sediments should be included in the studies on heavy metals, to obtain complete information on equilibrium and transport process taking place in the backwaters. 13 references, 12 figures.

  18. [Effect of the soil contamination with a potato cyst-forming nematode on the community structure of soil-inhabiting nematodes].

    Science.gov (United States)

    Gruzdeva, L I; Suzhchuk, A A

    2008-01-01

    Nematode community structure of the potato fields with different infection levels of potato cyst-forming nematode (PCN) such as 10, 30 and 214 cysts per 100 g of soil has been investigated. The influence of specialized parasite on nematode fauna and dominance character of different ecological-trophic groups were described. Parasitic nematode genera in natural meadow biocenosis and agrocenoses without PCN are Paratylenchus, Tylenchorhynchus, and Helicotylenchus. It is established, that Paratylenchus nanus was the prevalent species among plant parasites at low infection level. Larvae of Globodera prevailed in the soil with middle and high infection levels and substituted individuals of other genera of parasitic nematodes. The fact of increase in number of hyphal-feeding nematode Aphelenchus avenae was revealed. PMID:19198175

  19. [Ecological regularities of the existence of pathogenic Yersinia in soil ecosystems].

    Science.gov (United States)

    Breneva, N V; Maramovich, A S; Klimov, V T

    2005-01-01

    In this review the data on the ecology of pathogenic Yersinia in soil ecosystems, based on prolonged observations, were analyzed and summarized. In contrast to saprophytic species, ubiquitously spread in nature, pathogenic representatives of the genus Yersinia occurred only in the soil of natural foci and of these, Y. pestis were found only in the soil of burrows of the main carriers. The complex of abiotic and biotic factors (temperature, humidity, chemical composition, interactions in biocenosis) which determined the possibility of the existence of Yersinia in the soil environment and the preservation of their pathogenic properties was considered. Special attention was paid to their geno-phenotypic variability as the main factor of the adaptation of the causative agents of plague, pseudotuberculosis and intestinal yersiniosis in the environment. PMID:16438385

  20. [Taxonomic status of the Burana virus (BURV) (Bunyaviridae, Nairovirus, Tamdy group) isolated from the ticks Haemaphysalis punctata Canestrini et Fanzago, 1877 and Haem. concinna Koch, 1844 (Ixodidae, Haemaphysalinae) in Kyrgyzstan].

    Science.gov (United States)

    L'vov, D K; Al'khovskiĭ, S V; Shchelkanov, M Iu; Shchetinin, A M; Deriabin, P G; Gitel'man, A K; Aristova, V A; Botikov, A G

    2014-01-01

    Complete genome sequence of the Burana virus (BURV) was determined using the next-generation sequencing approach (ID GenBank KF801651). The prototype strain of BURV LEIV-Krg760 was originally isolated from the ticks Haemaphysalis punctata Canestrini et Fanzago, 1877 (Ixodidae, Haemaphysalinae), collected from cows in Tokmak wildlife sanctuary, eastern part of the Chu valley (43 degrees 10' N, 74 degrees 40' E) near Burana village, Kirgizia, in April 1971. Molecular genetics and phylogenetic analyses showed that the BURV belonged to the Nairovirus genus, Bunyaviridae and is related to Tamdy virus (TAMV) that is also associated with the ixodidae ticks of pasture biocenosis in Central Asia. Previous studies showed that TAMV is the prototypic virus of new phylogenetic Tamdy group in the Nairovirus genus. Thus, BURV was classified as a new virus of the Tamdy group, Nairovirus, Bunyaviridae. PMID:25549462

  1. [Immunomodulators for topical application to prevent and manage chronic adenoiditis in children].

    Science.gov (United States)

    Garashchenko, T I; Zelenkova, I V; Alferova, M V

    2011-01-01

    The authors report the results of a study on the efficacy of topical application of the immunomodulator IRS 19 in children presenting with chronic adenoiditis and grade I-III hypertrophy of adenoid vegetation. The use of this preparation is shown to faster and more efficaciously normalize the volume of the lymphoid tissue than irrigation of the nasopharynx with saline solutions. Moreover, the treatment of chronic adenoiditis with IRS 19 promoted normalization of biocenosis of the nasopharyngeal secretion and significantly decreased the abundance of pathogenic microflora. Specifically, the overall frequency of exacerbations and the frequency of exacerbations of adenoiditis decreased three- and two-fold respectively while the duration of the disease shortened. It is recommended that the topical immunomodulator IRS 19 should be included in the programs of planned seasonal treatment of children suffering chronic adenoiditis (to be applied at least 2-3 times annually). PMID:21512491

  2. Innate immunity underlies symbiotic relationships.

    Science.gov (United States)

    Kisseleva, E P

    2014-12-01

    Here, the modern data regarding interactions between normal microbiota and barrier tissues in plants, humans and animals are reviewed. The main homeostatic mechanisms responsible for interactions between epithelium and innate immune cells with symbiotic bacteria are described. A key step in this process is recognition of soluble microbial products by ligation to pattern-recognition receptors expressed on the host cells. As a result, epithelial cells secrete mucus, antibacterial peptides and immunoregulatory molecules. The main outcomes from immunological reactions towards symbiotic bacteria involve development of conditions for formation and maintenance of microbial biocenosis as well as providing safety for the host. Also, it is considered important to preserve and transfer beneficial bacteria to progeny. PMID:25716721

  3. [Microflora of the mucous membrane of human tonsils in the normal state and in pathology].

    Science.gov (United States)

    Khusnutdinova, L M

    2006-01-01

    A total of 50 healthy persons and 50 chronic tonsillitis patients were examined with the use of the bacteriological method. The microflora of tonsillar mucous membrane was represented by 8 genera of facultative anaerobic microorganisms. In the biocenosis of the tonsillar mucosa of the healthy subjects the occurrence of Streptococcus was 1.5-fold greater than in that of the patients; microorganisms of the genus Staphylococcus prevailed on the tonsillar mucosa of chronic tonsillitis patients 1.3-fold prevailed more often and Klebsiella and Neisseria prevailed 4-fold more often. Among the microorganisms isolated from the patients, cultures simultaneously having two or more pathogenicity markers occurred twice as often as among the representatives of the microbiocenosis obtained from the healthy subjects. The microflora of the tonsillar mucosa of the chronic tonsillitis patients was characterized by resistance to penicillins, macrolides and aminoglygosides, but was simultaneously sensitive to lincosamides and glycopeptides. PMID:16532644

  4. [Reciprocal formation of mucosal microflora of open cavities of different habitats in women as an important factor of their reproductive health].

    Science.gov (United States)

    Kungurtseva, E A; Popkova, S M; Leschenko, O Ya

    2014-01-01

    This review presents the current data on the human microbiome. In recent increase of erased and primary chronic forms of inflammatory diseases of the female genital organs is observed, in which the clinical manifestations are varied, but the symptoms are not so much related with the changes in the pelvic organs, but with abnormalities in other body systems, secondarily involved in the pathological process. Recent research proved mutual influence of intestinal, vaginal and nasopharyngeal biocenosis. Women with vaginal microbiota disorders often revealed intestinal dysbiosis and chronic diseases of ENT (Ear Nose Throat) organs, which in turn contributes to chronic inflammatory process of pelvic organs, failure of therapeutic interventions, reduced quality of life and increased risk of adverse pregnancy outcomes. PMID:25816640

  5. [An example of anthropogeneous succession in the ecosystem of the Verkhnetulomsky Water Reservoir (the Tuloma River Basin, the Kola region)].

    Science.gov (United States)

    Mitenev, V K; Shul'man, B S; Karasev, A B; Ponomarev, S V

    2010-01-01

    The destruction of initial biocenoses in the ecosystem of the Verkhnetulomsky Water Reservoir caused deep succession processes. As a result of the loss of the amphipod group in the benthos, replacement of the trophic links in fishes took place in the biocenosis of the reservoir. Benthos feeders changed over to the feeding on zooplankton and, as a result, became the constituent of the predators'diet. The structure of parasitic systems has changed. The parasites using amphipods as intermediate hosts have been and, on the contrary, the abundance of parasites infesting fishes through feeding of the latter on zooplankton has increased. Among the parasites, Triaenophorus crassus Forel became the cause of the appearance of stable triaenophorosis focus. PMID:21061595

  6. Primeros datos sobre el Medio Subterráneo Superficial y otros hábitats subterráneos transicionales en el País Vasco

    Directory of Open Access Journals (Sweden)

    GALAN, C.

    2001-01-01

    Full Text Available Se presentan resultados geomorfológicos y faunísticos prelimares de sondeos efectuados para el estudio del MSS (Medio Subterráneo Superficial en Gipuzkoa (País Vasco. La prospección ha sido efectuada sobre depósitos coluviales y clásicos de ladera y en dos biotopos transicionales, en diferentes litologías, de edades Paleozoico a Eoceno. Se ofrecen los primeros datos sobre biocenosis del MSS en la región. Entre la fauna hipógea han sido hallados colémbolos Entomobrydae, coleópteros Bathysciianae (Choleviadae : Leptodirinae, ácaros Rhagidiidae, hirudineos Gnatobdellida, y diversos representantes del medio edáfico.

  7. Development and testing of methods to be used in the Dreissena polymorpha (PALLAS) freshwater mussel for the biomonitoring of heavy metals in the ecosystem of the river Weser. Entwicklung und Erprobung von Methoden fuer einen Einsatz der Suesswassermuschel Dreissena polymorpha (PALLAS) fuer ein Biomonitoring von Schwermetallen im Oekosystem Weser

    Energy Technology Data Exchange (ETDEWEB)

    Busch, D.

    1991-03-01

    The suitability of the Dreissena polymorpha freshwater mussel for passive and active biomonitoring of heavy metals (Pb, Cd, Cr, Ni, Zn, Cu, Hg) in a limnic ecosystem, particularly that of the Weser, was investigated from different points of view. The local and temporal differences in concentration seen for the soft parts reflect the variations in the bioavailability of heavy metals between sites of exposure and are not to be regarded as a result of the mussels' individual living conditions. Bioavailability is an independent quantitative factor that is subject to local and temporal changes and of key importance to ecological risk assessments. It cannot be determined or extrapolated from the chemico-technical values usually measured for various water parameters. An analysis of the concentrations of pollutants in the water and sediment does not provide a basis for reliable predictions about the related dangers to the particular biocenosis constituted by waters. (orig.).

  8. [Significance of hydrobiont persistent properties for symbiotic interactions].

    Science.gov (United States)

    Nemtseva, N V

    2012-01-01

    Significance of symbiotic relations formed by associative symbiosis type for autochthonous and allochthonous microflora of natural water bodies is shown. Generality of symbiotic interaction mechanisms of symbionts in limnetic and halophilous communities provided by secreted factors of natural resistance from the side of the host, and by factors of persistence from the side of symbionts is proven based on a set of examples. Features of operation of lysozyme-antilysozyme, histon-antihiston, hydrogen peroxide-catalase functional systems in symbiotic interactions of autotrophic and heterotrophic components of hydrobiocenosis with dominant and associative microflora are presented. Associative microflora of allochthonous origin was shown to actively use the ecologically formed system of interaction between hydrobionts that facilitates survival of these microorganisms and preservation of their persistent potential, and as a result leads to biocenosis disorders. The knowledge obtained open new possibilities and perspectives of research of sanitary and ecological aspects of vital activity of aquatic biocenoses. PMID:22937711

  9. Structure, seasonal dynamics and distribution of zooplankton in lake Drukshiai in 1994

    International Nuclear Information System (INIS)

    Investigations on the zooplankton of Lake Drukshiai were carried out in 1994. There were registered 62 taxons of protozoa and 50 taxons of metazoa, and compared with the data of 1979 - 1986 the diversity of species composition decreased 2.1 times. Eurytermic and stenothermic thermophylic species prevailed in the plankton biocenosis. In protozooplankton dominated ciliates of subclasses teolotricha and spirotricha, in metazooplankton -planctonic crustacea (Copopeda and Cladocera). Rotifers (Rotaria) were abundant only in the shallow and heated water outlet area. Seasonal dynamics of protozooplankton indicated one maximum in spring and metazooplankton - in summer. High diferentiation in quantity and biomass of zooplankton in the lake revealed different level of eutrophication of some areas. (author). 7 refs., 5 figs

  10. Research project on the thermal pollution of waters

    International Nuclear Information System (INIS)

    The results of essentially completed and current research and development projects - as far as available in a short time - are explained in the present study, compared and their practicle applicability indicated. The number of publications in the literature index is split up into the single specialist fields as follows: 13% hydrodynamics (propagation caculations, models, measurements); 45% biology-chemistry (effects on micro and macro fauna of waters, on water contents, mathematical models of oxygen balance and biocenosis); 31% hydrometeorology including problems on the thermal economy of the waters as well as special thermal load calculations; 5% heat introduction into ground water; 6% others e.g. use of remote sensing for temperature measurement. The current research projects in the FRG are split up into the following single specialist fields: 16% hydromechanics; 42% biology-chemistry; 24% hydrometeorology including thermal economy; 10% use of ground water; 8% others (almost exclusively problems in connection with the use of remote sensing methods). (orig.)

  11. Microbial community structure of activated sludge in treatment plants with different wastewater compositions

    Directory of Open Access Journals (Sweden)

    Nataliya M. Shchegolkova

    2016-02-01

    Full Text Available Activated sludge (AS plays a crucial role in the treatment of domestic and industrial wastewater. AS is a biocenosis of microorganisms capable of degrading various pollutants, including organic compounds, toxicants and xenobiotics. We performed 16S rRNA gene sequencing of AS and incoming sewage in three wastewater treatment plants (WWTPs responsible for processing sewage with different origins: municipal wastewater, slaughterhouse wastewater, and refinery sewage. In contrast to incoming wastewater, the taxonomic structure of AS biocenosis was found to become stable in time, and each WWTP demonstrated a unique taxonomic pattern. Most pathogenic microorganisms (Streptococcus, Trichococcus, etc. and inductors of AS bulking and flocculation (Comamonadaceae, Flavobacteriaceae, etc., which are abundantly represented in incoming sewage, were significantly decreased in AS of all WWTPs, except for the slaughterhouse wastewater, which was rich in organic matter. Here we present a novel approach enabling the prediction of the metabolic potential of bacterial communities based on their taxonomic structures and MetaCyc database data. We developed a software application, XeDetect, to implement this approach. Using XeDetect, we found that the metabolic potential of the three bacterial communities clearly reflected the substrate composition. We revealed that the microorganisms responsible for AS flocculation and bulking (abundant in AS of slaughterhouse wastewater played a leading role in the degradation of substrates such as fatty acids, amino acids, and other bioorganic compounds. Moreover, we discovered that the chemical, rather than the bacterial composition of the incoming wastewater was the main factor in AS structure formation. XeDetect (freely available: https://sourceforge.net/projects/xedetect represents a novel powerful tool for the analysis of the metabolic capacity of bacterial communities. The tool will help to optimize bioreactor performance and

  12. Microbial Community Structure of Activated Sludge in Treatment Plants with Different Wastewater Compositions.

    Science.gov (United States)

    Shchegolkova, Nataliya M; Krasnov, George S; Belova, Anastasia A; Dmitriev, Alexey A; Kharitonov, Sergey L; Klimina, Kseniya M; Melnikova, Nataliya V; Kudryavtseva, Anna V

    2016-01-01

    Activated sludge (AS) plays a crucial role in the treatment of domestic and industrial wastewater. AS is a biocenosis of microorganisms capable of degrading various pollutants, including organic compounds, toxicants, and xenobiotics. We performed 16S rRNA gene sequencing of AS and incoming sewage in three wastewater treatment plants (WWTPs) responsible for processing sewage with different origins: municipal wastewater, slaughterhouse wastewater, and refinery sewage. In contrast to incoming wastewater, the taxonomic structure of AS biocenosis was found to become stable in time, and each WWTP demonstrated a unique taxonomic pattern. Most pathogenic microorganisms (Streptococcus, Trichococcus, etc.), which are abundantly represented in incoming sewage, were significantly decreased in AS of all WWTPs, except for the slaughterhouse wastewater. Additional load of bioreactors with influent rich in petroleum products and organic matter was associated with the increase of bacteria responsible for AS bulking and foaming. Here, we present a novel approach enabling the prediction of the metabolic potential of bacterial communities based on their taxonomic structures and MetaCyc database data. We developed a software application, XeDetect, to implement this approach. Using XeDetect, we found that the metabolic potential of the three bacterial communities clearly reflected the substrate composition. We revealed that the microorganisms responsible for AS bulking and foaming (most abundant in AS of slaughterhouse wastewater) played a leading role in the degradation of substrates such as fatty acids, amino acids, and other bioorganic compounds. Moreover, we discovered that the chemical, rather than the bacterial composition of the incoming wastewater was the main factor in AS structure formation. XeDetect (freely available: https://sourceforge.net/projects/xedetect) represents a novel powerful tool for the analysis of the metabolic capacity of bacterial communities. The tool will

  13. RNA/DNA ratio as biomarkers for periphyton and macroinvertebrate growth

    Science.gov (United States)

    Mewes, Daniela; Winkelmann, Carola

    2015-04-01

    A biocenosis is a complex assembly of organisms driven and shaped by numerous processes and interactions. Yet, in order to describe the biocenosis of a stream often only state variables, such as algal biomass or invertebrate diversity and abundance, are measured. But these variables fail to provide much needed information on those driving processes. Because processes such as growth of periphyton and invertebrates can hardly be measured directly in the field, the use of biomarkers is a promising approach to quantify biological rates under natural conditions. Periphyton represents the main food source for invertebrate grazers and periphyton growth rate rather than standing stocks alone allows the estimation of the availability of this resource. A linear relationship of RNA/DNA ratios and growth rate has previously been established for single species cultures of algae and bacteria but not for naturally occurring freshwater periphyton assemblages. In this study it could be shown that linear relationships of RNA/DNA ratios and growth rate are also valid for naturally occurring freshwater periphyton assemblages and can be used as biomarkers for periphyton growth rate. Moreover, recent results indicate that the RNA/DNA ratio might also be used as biomarker for invertebrates, because high-quality food was observed to increase the RNA/DNA ratios of the freshwater amphipod Dikerogammarus villosus. These are very promising results with regard to the usefulness and applicability of biomarkers ecosystem analysis in running waters. Additional biomarkers allowing the analysis of further processes and interactions within the food web such as PLFAs (phospholipid-fatty acids), neutral lipids and PUFAs (polyunsaturated fatty acids) are to be tested for their applicability in stream ecosystems.

  14. An ecological approach supporting the management of sea-uses and natural capital in marine coastal areas

    Science.gov (United States)

    Marcelli, Marco; Carli, Filippo M.; Bonamano, Simone; Frattarelli, Francesco; Mancini, Emanuele; Paladini de Mendoza, Francesco; Peviani, Maximo; Piermattei, Viviana

    2015-04-01

    The purpose of our work is to create a multi-layer map of marine areas and adjacent territories (SeaUseMap), which takes into account both the different sea uses and the value of marine ecosystems, calculated on the basis of services and benefits produced by the different biocenosis. Marine coastal areas are characterized by the simultaneous presence of ecological conditions favorable to life and, at the same time, they are home to many human activities of particular economic relevance. Ecological processes occurring in coastal areas are particularly important and when we consider their contribution to the value of the "natural capital" (Costanza et Al. 1997, 2008, 2014), we can observe that this is often higher than the contribution from terrestrial ecosystems. Our work is done in northern Lazio (Civitavecchia), a highly populated area where many uses of the sea are superimposed: tourism, fisheries, industry, shipping and ports, historical and cultural heritage. Our goal is to create a tool to support decision-making, where ecosystem values and uses of the sea can be simultaneously represented. The ecosystem values are calculated based on an analysis of benthic biocoenoses: the basic ecological units that, in the Mediterranean Sea, have been identified, defined, analyzed and used since the 60s (Perez & Picard 1964) to date as a working tool (Boudouresque & Fresi 1976). Land surface, instead, was analyzed from available maps, produced within the Corine Land Cover project. Some application examples to support the decision-making are shown, with particular reference to the localization of suitable areas for wave energy production and the esteem of ecological damages generated in case of maritime accidents (e.g., Costa Concordia). According to Costanza 2008, we have developed our own operational method, which is suitable for this specific case of benefit assessment from benthic communities. In this framework, we base our strategy on the ability of the benthic

  15. Water bodies typology system: a Chilean case of scientific stakeholders and policy makers dialogue

    Directory of Open Access Journals (Sweden)

    Rodrigo Fuster

    2012-12-01

    Full Text Available The aim of this project was to obtain a scientists-validated Typology System, which would allow to classify the surface waters bodies in Chile and, therefore, to facilitate the environmental institutional water management in the country. For this, during the years 2009 and 2011, a Typology System for the surface freshwater bodies was developed for Chile based on the methodology described by the Water Framework Directive of the European Union, which was adapted to local features through the knowledge of limnologist experts in the country, as well as policy makers' experience and their management requirements . In a first stage, national ecoregions were developed and abiotic variables were defined to compose the Typology System. The resulted Typology System for lakes and rivers was generated following an a priori and top down approach to difference biocenosis, based on geomorphologic, hydrologic and physic criteria. In a second stage, the proposed Typology System was validated by experts and policy makers, in which process new arrangements were included in the system. The working methodology used for both stages was bibliographic review, interviews to local experts in biocenosis and workshops. It is specially highlighted the participative processes and discussions in which all the agents involved were present, all of which resulted in the creation of a valid system from a scientific point of view and a product that is applicable to the necessities of the environmental institutions of the country. This work represents a successful experience in the improvement of the communication between scientists and politicians in Chile, which is a relevant factor for the elaboration of more efficient and effective environmental policies, integrating not only management and economic issues, but also more technical aspects that can influence in the final success of any long term strategy. For this reason, the replication of this kind of experiences, as well as

  16. Synthesis of Organic Matter of Prebiotic Chemistry at the Protoplanetary Disc

    Science.gov (United States)

    Snytnikov, Valeriy; Stoynovskaya, Olga; Rudina, Nina

    We have carried out scanning electron microscopic examination of CM carbonaceous chondrites meteorites Migey, Murchison, Staroe Boriskino aged more than 4.56 billion years (about 50 million years from the beginning of the formation of the Solar system). Our study confirmed the conclusion of Rozanov, Hoover and other researchers about the presence of microfossils of bacterial origin in the matrix of all these meteorites. Since the time of the Solar system formation is 60 - 100 million years, the primary biocenosis emerged in the protoplanetary disc of the Solar system before meteorites or simultaneously with them. It means that prebiological processes and RNA world appeared even earlier in the circumsolar protoplanetary disc. Most likely, this appearance of prebiotic chemistry takes place nowday in massive and medium-massive discs of the observed young stellar objects (YSO) class 0 and I. The timescale of the transition from chemical to biological evolution took less than 50 million years for the Solar system. Further evolution of individual biocenosis in a protoplanetary disc associated with varying physico-chemical conditions during the formation of the Solar system bodies. Biocenosis on these bodies could remove or develop under the influence of many cosmic factors and geological processes in the case of Earth. To complete the primary biosphere formation in short evolution time - millions of years - requires highly efficient chemical syntheses. In industrial chemistry for the efficient synthesis of ammonia, hydrogen cyanide, methanol and other organic species, that are the precursors to obtain prebiotic compounds, catalytic reactors of high pressure are used. Thus (1) necessary amount of the proper catalyst in (2) high pressure areas of the disc can trigger these intense syntheses. The disc contains the solids with the size from nanoparticle to pebble. Iron and magnesium is catalytically active ingredient for such solids. The puzzle is a way to provide hydrogen

  17. Feeding habits of a dormouse population (Myoxus glis of the Asiago Plateau (Venetian Prealps / Abitudini alimentari di una popolazione di Ghiro (Myoxus glis dell'altopiano di Asiago (Prealpi venete

    Directory of Open Access Journals (Sweden)

    Daniel Franco

    1990-07-01

    Full Text Available Abstract A new and simplified method was used to investigate on the feeding habits of a Dormouse population of the Asiago Plateau, Venetian Prealps; it is based on the volume evaluation of plant and animal components of the stomach contents by stain proceedings and by the identification of the large-sized remains. The population studied has more carnivorous habits than others cited in the literature, and presents an increasing percentage of animal food in stomach contents during: (1 the summer decrease in body weight (linked to an higher energy consumption, (2 sexual activity and (3 the period preceding hibernation. The diet variety is lower and the ground level predation higher than those reported for other populations. This anomalous trophic behaviour is probably a consequence of changed conditions of the biocenosis, originally based on Beech stands and now on artificial pure stands of Norway Spruce, which is not frequently utilized as food. The adaptation to this particular diet is prompt and general, and it must be related to social learning. Riassunto Per studiare il comportamento trofico di una popolazione di Ghiro (Myoxus glis L. dell'Altopiano di Asiago, Prealpi Venete, è stato utilizzato un metodo nuovo e semplificato rispetto ai precedenti, basato sulla valutazione dei volumi della componente animale e vegetale mediante analisi di tipo colorimetrico, e sulla classificazione dei residui di maggiori dimensioni dei contenuti stomacali. La popolazione in esame è risultata di abitudini più carnivore (con differenze tra i maschi e le femmine rispetto a popolazioni studiate da altri autori, presentando una maggiore percentuale di cibo animale nel contenuto stomacale durante la diminuzione estiva in peso corporeo (associata a sua volta ad un elevato consumo energetico, durante l'attività sessuale e prima del letargo. Anche la varietà della dieta e la predazione a livello del suolo sembrano essere

  18. A review on the effects of environmental conditions on growth and toxin production of Ostreopsis ovata.

    Science.gov (United States)

    Pistocchi, R; Pezzolesi, L; Guerrini, F; Vanucci, S; Dell'aversano, C; Fattorusso, E

    2011-03-01

    Since the end of the 1990s the occurrence of blooms of the benthic dinoflagellates Ostreopsis spp. is spreading in many tropical and temperate regions worldwide, sometimes causing benthonic biocenosis suffering and occasional human distress. Ostreopsis ovata has been found to produce palytoxin-like compounds, a class of highly potent toxins. As general, the highest abundances of Ostreopsis spp. are recorded during warmer periods characterized by high temperature, salinity, and water column stability. Moreover, as these cells are easily resuspended in the water column, the role of hydrodynamism in the blooms development and decline has been highlighted. The environmental conditions appear, therefore, to be one of the main factors determining the proliferation of these species as testified by several field surveys. Laboratory studies on the effect of environmental parameters on growth and toxicity of O. ovata are rather scarce. With regard to the effects of temperature, culture results indicate that different strains blooming along Italian coasts displayed different optima, in accordance to blooming periods, and that higher toxin levels correlated with best growth conditions. Additionally, in relation to an Adriatic strain, cell growth positively correlated with the increase in salinity, while toxicity was lowest at the highest salinity value (i.e. 40). For the same strain, both nitrogen and phosphorus limitation determined a decrease in cell toxicity showing different behaviour with respect to many other toxic dinoflagellates. PMID:20920514

  19. Validation trial of Japan's zinc water quality standard for aquatic life using field data.

    Science.gov (United States)

    Matsuzaki, Kanae

    2011-10-01

    This study sought to validate Japan's zinc water quality standard for aquatic life (algae and benthic invertebrates) based on field survey data. The effects of zinc on aquatic life, especially algae and benthic invertebrates, were investigated mainly in water areas with upstream basins that contain mines. Seven biological indicators (number of cells or individuals, number of taxa, number of EPT taxa, number of collector-gatherer taxa of benthic invertebrates, and Simpson index, Shannon-Weiner index, and Margalef index for algae and benthic invertebrates) were analyzed with respect to zinc concentrations and the zinc toxic equivalent quantity (Zn-TEQ), and additive contribution from other metals was assumed. The results showed that the number of taxa of algae and benthic invertebrates significantly decreased with increases in zinc concentration and Zn-TEQ. For benthic invertebrates, six of the metrics (all except the number of individuals) tended to decrease with increases in zinc concentration and Zn-TEQ. The effect level of biocenosis (ELB) was defined as the concentration at which the metrics decrease significantly with Wilcoxon's rank sum test. The ELB calculated for zinc was in the range of 16-54 μg/L for zinc concentration and 38-50 μg/L for Zn-TEQ; thus, Japan's environmental zinc standard for the protection of aquatic life, at 30 μg/L, was found to be a level consistent with these results. PMID:21851980

  20. [The detection of occurrence rate of genes coding capability to form pili binding in auto-strains of Escherichia coli].

    Science.gov (United States)

    Ivanova, E I; Popkova, S M; Dzhioev, Iu P; Rakova, E B; Dolgikh, V V; Savel'kaeva, M V; Nemchenko, U M; Bukharova, E V; Serdiuk, L V

    2015-01-01

    E. coli is a commensal of intestine of the vertebrata. The exchange of genetic material of different types of bacteria between themselves and with other representatives of family of Enterobacteriaceae in intestinal ecosystem results in development of types of normal colibacillus with genetic characteristics of pathogenicity that can serve as a theoretical substantiation to attribute such strains to pathobionts. The entero-pathogenic colibacillus continues be an important cause of diarrhea in children in developing countries. The gene responsible for formation of pili binding is a necessary condition for virulence of entero-pathogenic colibacillus. The polymerase chain reaction was applied to examine 316 strains of different types of E. coli (normal, with weak enzyme activity and hemolytic activity) isolated from healthy children and children with functional disorders of gastro-intestinal tract for presence of genes coding capability to form pill binding. The presence of this gene in different biochemical types of E. coli permits to establish the fact of formation of reservoir of pathogenicity in indigent microbiota of intestinal biocenosis. PMID:25874306

  1. Compatibility and verdure of seedling for autochtonous peer in the region of north Montenegro

    Directory of Open Access Journals (Sweden)

    Šebek Gordana

    2014-01-01

    Full Text Available The study was conducted in three successive years to examine the compatibility and verdure in nursery trees of autochthonous peer cultivars grafted on Quince MA and wild peer seedlings (Pyrus communis L during the first year after bud grafting. The aim of this paper is to determine compatibility and verdure of autochthonous peer seedling sorts (Pećanka, Jerebasma, Lubeničarka, Sinka, Turšijača and Jarac with the Quince MA and wild peer seedlings (Pyrus communis L. The severity of incompatibility varies between varieties but is always more severe with Quince MC than Quince MA. The incompatibility expresses itself in several ways. Firstly a poor ‘bud’ or ‘graft’ take in the nursery will be evident, secondly even if growing successfully in the nursery very often when ‘lifted’ breakages (brittle unions occur between rootstock and scion and thirdly ‘delayed’ incompatibility where again the union between rootstock and scion breaks suddenly in later years, this is unpredictable and can often happen when there is a heavy crop load assisted by strong autumnal winds. The production of seedling material of autochthonous sorts with vegetative rootstocks Quince MA will be enormous contribution for even partially saving of fruit genofond that is the unity product of our ecological environment and autochthonous biocenosis.

  2. Assessment of Kerch Bay environmental pollution using neuroglial proteins of ground fish

    Directory of Open Access Journals (Sweden)

    H. V. Sukharenko

    2014-03-01

    Full Text Available The modern ecology situation in waters of the Kerch Strait requires assessment of disturbances in biotopes and monitoring of the degree of impact of industrial pollutants on ecosystem. Deposit of oil products after the 2007 year ships’ accidents might have considerable impact on the water biocenosis area. The investigation of cytoskeleton marker of astrocytes glial fibrillary acidic protein (GFAP in brain of the bullhead (Neogobius fluviatilis, which is the typical representative of the commercial ground fish of the Kerch Strait, has been carried out. The results of comparative analysis of GFAP content in the brain of fish from the Kerch Bay near-shore waters and fish from conditionally clear area of Vorskla river shows the reliable (2.18 times increasing of GFAP in the area of industrial pollution. Rising GFAP content indicates the astrogliosis development as a result of metabolic disturbances which can be induced by higher content of oil products in the near-bottom biotopes of the Kerch Bay. Increase in lipid peroxidation level was observed in the brain of fish from the Kerch Bay. The results provided with regard to violations of the state of astrocyte cytoskeleton and oxidative stress in the brain of bullhead from the Kerch Bay prove the sublethal biology effect of industrial pollutants in hydrobionts from this area. Results of this investigation also indicate the necessity of continuous ecology monitoring and comprehensive study of hydrobiont populations in the industrial regions and ecological disaster zones.

  3. Diversity as a measure of water pollution and an aid for biological water analysis

    Energy Technology Data Exchange (ETDEWEB)

    Verma, S.R.; Sharma, A.K.; Goel, D.P.

    1987-01-01

    Five aquatic ecosystems, Yamuna river, Krishna river Eastern Kalinadi, Kadrabad drain and Peacock lake have been studied with reference to disversity and physico-chemical characteristics and biological indices; a list of species have been selected with reference to polluted, mildly polluted or unpolluted water conditions. Though it is difficult to call any species strictly indicator species, certain species of Bacillariophyceae, bottom biota and Entomostraca can be regarded as indicative species; the quantitative distributions of the species of different groups have been analysed statistically. Margalef's Community Diversity Index (d), Shannon Weaver Function, coefficient of rank correlation and partial and multiple correlation coefficient were calculated as to find out the order of precedence in different taxonomical groups and the linear regression against BOD and the whole biocenosis were calculated, which indicate the relation as diversity d=6.7854-0.0080 BOD+-0.9695 where r/sup 2/=B=0.7365. Similarly, multiple linear regressions were also calculated using diversity against BOD, pH and temperature for each aquatic ecosystem. All these observations indicate that diversity of organisms can be used to measure the water pollution intensity.

  4. Soil arthropods as test organisms for ecotoxicology

    Energy Technology Data Exchange (ETDEWEB)

    Iglisch, I.

    1981-02-01

    The importance of arthropods - organisms which usually grow in masses - for soil biology depends on their capacity to participate in the continuous transformation of organic substances within the relevant biocenosis and thus to take part in the maintenance of the ecological balance. In ecotoxicology, i.e. the science of substances having a detrimental effect on the natural balance of ecosystems, we try to find ways to evaluate risk of substances hazardous to the environment. In principle, biocenoses would offer themselves in their entirety as appropriate test objects for ecotoxicological evaluation of chemicals. Since it will not yet be possible in the near future to carry out this kind of studies, individual organisms proved as representatives of terrestial biotopes have to be chosen for these purposes. Primarily, Collembola, Coleoptera, and Diptera (larvae) are part of the meso- and macrofauna of soil arthropods or soil insects according to the experience made up to now in respect of their importance for soil biology. Representatives of such organisms should be used to develop test procedures to indicate damage even of a subacute, chronic nature or the impairment of their functional performance the maintance of which is a prerequisite for the ecological balance.

  5. Significance of wildfowl populations and their parasite fauna

    Directory of Open Access Journals (Sweden)

    Ilić Tamara

    2013-01-01

    Full Text Available Populations of wildfowl have been categorised taxonomically into five families: Phasianidae, Tetraonidae, Scolopacidae, Otidae and Anatidae. They are of particular importance for agriculture, forestry and the game economy, and certain species also have an important role in the epizootiology of parasitic infections in domestic fowl and fish. That is why it is extremely important for the process of promoting the system of animal health protection to have knowledge regarding the etiology and epizootiology of parasitic infections in wildfowl. Diseases in wildfowl caused by protozoa are histomoniasis, trichomoniasis, malaria, coccidiosis, cryptosporidiosis, sarcocystosis, toxoplasmosis, and giardiasis. The most represented helminthiases in wildfowl are singamosis, capillariasis, trichostrongyliasis, ascaridiosis, heteraciosis, nematodosis of the glandular stomach, cestodoses and trematodoses. The most significant ectoparasites in wildfowl are mites, ticks, and fleas. The reduction of populations of certain widlfowl species in nature could present a major problem, having in mind the important role of these birds in ecosystems and biocenosis. The incidence, maintaining and spreading of parasitic infections among widlfowl are just some of the factors that affect the numbers of their populations.

  6. [The efficacy of the combined rehabilitative treatment of the patients presenting with obesity, metabolic syndrome, and diseases of the hepatobiliary system].

    Science.gov (United States)

    Cherchinian, A S

    2012-01-01

    A total of 80 patients presenting with exogenous constitutional obesity, metabolic syndrome, and diseases of the hepatobiliary system (including chronic non-calculous cholecystitis and fatty hepatosis) were enrolled to participate in the present study. The basal treatment consisted of the adequate reducing diet, remedial gymnastics, massage, reflexo-acupuncture, the application of galvanic muds, and controlled intake of chofitol. It was supplemented with magnetic laser irradiation of selected abdominal regions, and electrical stimulation of femoral, dorsal, and abdominal muscles. It was shown that the combination of the above procedures and physical factors significantly improves the overall outcome of the treatment. The patients suffering intestinal dysbacteriosis were prescribed the intake of probiotic Nor Narine together with Jermuk mineral water; they were found to benefit from such treatment due to normalization of intestinal biocenosis and improvement of their general condition. The clinical and paraclinical data obtained in this study give evidence of the therapeutic efficacy of certain physical factors and especially their combination used for the medical rehabilitation of the patients presenting with constitutional obesity, metabolic syndrome, and digestive disorders. Moreover, the well-apparent positive results were documented from the combined treatment with magnetic laser radiation and therapeutic muds. PMID:23210358

  7. Palaeoanellus dimorphus gen. et sp. nov. (Deuteromycotina): a Cretaceous predatory fungus.

    Science.gov (United States)

    Schmidt, Alexander R; Dörfelt, Heinrich; Perrichot, Vincent

    2008-10-01

    In habitats where nitrogen is the limiting factor, carnivorous fungi gain an advantage by preying on nematodes and other microorganisms. These fungi are abundant in modern terrestrial ecosystems, but they are not predestined for preservation as fossils. Conclusions on their evolutionary history are therefore mainly based on molecular studies that are generally limited to those taxa that have survived until today. Here we present a fossil dimorphic fungus that was found in Late Albian amber from southwestern France. This fungus possessed unicellular hyphal rings as trapping devices and formed blastospores from which a yeast stage developed. The fossil probably represents an anamorph of an ascomycete and is described as Palaeoanellus dimorphus gen. et sp. nov. Because predatory fungi with regular yeast stages are not known from modern ecosystems, the fungus is assumed to not be related to any Recent carnivorous fungus and to belong to an extinct lineage of carnivorous fungi. The inclusions represent the only record of fossil fungi that developed trapping devices, so far. The fungus lived c. 100 million years ago in a limnetic-terrestrial microhabitat, and it was a part of a highly diverse biocenosis at the forest floor of a Cretaceous coastal amber forest. PMID:21632336

  8. Psychrophilic Biomass Producers in the Trophic Chain of the Microbial Community of Lake Untersee, Antarctica

    Science.gov (United States)

    Pikuta, Elena V.; Hoover, Richard B.

    2010-01-01

    The study of photosynthetic microorganisms from the Lake Untersee samples showed dispersed distribution of phototrophs within 80 m water column. Lake Untersee represents a unique ecosystem that experienced complete isolation: sealed by the Anuchin Glacier for many millennia. Consequently, its biocenosis has evolved over a significant period of time without exchange or external interaction with species from other environments. The major producers of organic matter in Lake Untersee are represented by phototrophic and chemolithotrophic microorganisms. This is the traditional trophic scheme for lacustrine ecosystems on Earth. Among the phototrophs, diatoms were not found, which differentiates this lake from other known ecosystems. The dominant species among phototrophs was Chlamydomonas sp. with typical morphostructure: green chloroplasts, bright red round spot, and two polar flagella near the opening. As expected, the physiology of studied phototrophs was limited by low temperature, which defined them as obligate psychrophilic microorganisms. By the quantity estimation of methanogenesis in this lake, the litho-autotrophic production of organic matter is competitive with phototrophic production. However, pure cultures of methanogens have not yet been obtained. We discuss the primary producers of organic matter and the participation of our novel psychrophilic homoacetogen into the litho-autotrophic link of biomass production in Lake Untersee.

  9. [The symbiotic microflora associated with the tegument of proteocephalidean cestodes and the intestines of their fish hosts].

    Science.gov (United States)

    Korneva, Zh V; Plotnikov, A O

    2006-01-01

    The indigenous symbiotic microflora associated with the tegument of proteocephalidean cestodes and the intestines of their fish hosts has been investigated in morphological and ecological aspects. The indigenous microflora associated with the cestode tegument consists of the nannobacteria population, which was present obligatorily on the surface of tegument, and the "deep microflora". The deep microflora associates with some few species of parasites only. Each individual host-parasite micro-biocenosis includes specific indigenous symbiotic microorganisms, with the differing microfloras of host intestine and parasite. Physiology, biochemistry and/or diet of hosts apparently influence on the symbiotic microflora's structure of parasites. The least bacteria abundance and diversity of their morphotypes were observed in the parasites from baby fishes. The diversity and abundance of bacteria were increased with the fish host ageing and the formation of the definitive structure of its intestine. It is an evidence of the gradual invading of the intestinal parasites (cestodes) tegument by bacterial cells. The invading is realized on the base of the microflora that was present in the food of fish host. The symbiotic microflora has specific morphological features, can regulate the homeostasis of the cestodes and fish hosts and also can maintain equilibrium of alimentary and immune interrelations in the host-parasite system. PMID:17042276

  10. Water quality of Danube Delta systems: ecological status and prediction using machine-learning algorithms.

    Science.gov (United States)

    Stoica, C; Camejo, J; Banciu, A; Nita-Lazar, M; Paun, I; Cristofor, S; Pacheco, O R; Guevara, M

    2016-01-01

    Environmental issues have a worldwide impact on water bodies, including the Danube Delta, the largest European wetland. The Water Framework Directive (2000/60/EC) implementation operates toward solving environmental issues from European and national level. As a consequence, the water quality and the biocenosis structure was altered, especially the composition of the macro invertebrate community which is closely related to habitat and substrate heterogeneity. This study aims to assess the ecological status of Southern Branch of the Danube Delta, Saint Gheorghe, using benthic fauna and a computational method as an alternative for monitoring the water quality in real time. The analysis of spatial and temporal variability of unicriterial and multicriterial indices were used to assess the current status of aquatic systems. In addition, chemical status was characterized. Coliform bacteria and several chemical parameters were used to feed machine-learning (ML) algorithms to simulate a real-time classification method. Overall, the assessment of the water bodies indicated a moderate ecological status based on the biological quality elements or a good ecological status based on chemical and ML algorithms criteria. PMID:27191562

  11. [Antibiotical prophylaxy in gynecology].

    Science.gov (United States)

    Záhumenský, J; Menzlová, E; Zmrhal, J; Kučera, E

    2013-08-01

    Gynecological surgery is considered to be clear with possible contamination by gram-positive cocci from the skin, gram-negatives from the perineum or groins or polymicrobial biocenosis from vagina, depending on the surgical approach. Antibiotical prophylaxy enforces the natural mechanisms of immunity and helps to exclude present infection. There were presented many studies comparing useful effect of prophylaxis in gynecological surgery. The benefits of antibiotical prophylaxy before IUD insertion, before the cervical surgery and before hysteroscopies were not verified. On the other hand the prophylaxy of vaginal surgery including vaginal hysterectomy decreases the number of postoperative febrile complications. The positive influence of prophylaxis before the simple laparoscopy and laparoscopy without bowel injury or the opening of the vagina was not evidently verified. In abdominal hysterectomy the antibiotical prophylaxy decreases the incidence of postoperative complications significantly. The administration of 2 g of cefazolin can be recommended. In procedures taking more than 3 hours the repeated administration of cefazolin is suitable. New urogynecological procedures, using mesh implants, were not sufficiently evaluated as for postoperative infections and the posible antibiotical effect. The presence of implant in possibly non sterile area should be considered as high risc of postoperative complications. PMID:24040985

  12. Influence of gamma irradiation on the metabolic activity of sulfate-reducing bacteria

    Energy Technology Data Exchange (ETDEWEB)

    Agaev, N.M.; Guseinov, M.M.; Smorodin, A.E.

    1985-09-01

    When water is pumped into oil-bearing seams to increase oil production, the microorganisms in the injected water fall into favorable ecological conditions and, quickly adapting, form a biocenosis and begin to actively develop. Among the anaerobic microorganisms, the most hazardous from the corrosion viewpoint are the sulfate-reducing bacteria (SRB), which are the main producers of hydrogen sulfide as the product of anaerobic respiration. This paper reports on the effect of gamma rays on the metabolic study of SRB Desulfovibrio desulfuricans in the nutrient medium Postgate B. The radioactive source used is a /sup 60/CoK-125 unit with a power of 700 rad/sec. The required dose of gamma rays was calculated from the exposure times of samples with the test medium in the radiation zone o the isotope /sup 60/Co. The criterion characterizing the effectiveness of suppression of development of the bacteria is the concentration of biogenic hydrogen sulfide produced, as determined by iodometric titration.

  13. Clinicodiagnostic value of functional morphological changes of intestine in encephalopathy development in cirrhosis

    Directory of Open Access Journals (Sweden)

    V.V. Ovsyannikova

    2010-06-01

    Full Text Available The aim of the article is to study frequency and expressiveness of clinico-morphological and dysbiotic intestinal changes in cirrhosis and to estimate its interrelation with manifestations of hepatic encephalopathy. 146 patients with cirrhosis ages 20 to 70 years have been examined. The cirrhosis has been verified by standard methods. Examina¬tion included colonoscopy and stool specimen. Diagnostics of hepatic encephalopathy was based on clinical and psychometric methods. It is established that symptoms of intestinal dyspepsia often occurred in patients with cirrhosis of classes В and С by Chaild-Pugh. According to the endoscopic examination lesions of mucous membrane of large intestine have been revealed in 82% of patients with liver cirrhosis. Morphological research revealed changes in 100% of patients with cirrhosis. Frequency and expressiveness of changes in mucous membrane of large intestine depended on the degree of cirrhosis decompensation. Changes of biocenosis of large intestine were revealed in 93% of patients with cirrhosis, hepatic encephalopathy was diagnosed in 88% of patients, more frequently hepatic encephalopathy of stage I. The cirrhosis is accompanied by structural and functional changes of intestine that make up additional risk factor of development of hepatic encephalopathy

  14. The multifunctional artificial reef and its role in the defence of the Mediterranean coast.

    Science.gov (United States)

    López, I; Tinoco, H; Aragonés, L; García-Barba, J

    2016-04-15

    Multifunctional artificial reefs (MFAR) are being implemented around the world, due to their ability to provide an environment where a sports-economic-recreational use and environmental improvement is implemented, and are also elements of coastal defence. However, a lot of failures have been recorded, possibly due to disregarded local factors in the formulations used, and there is no method that has encompassed all these factors, in order to take them into account in its design. The aim of this paper was to provide the coastal engineer with a method that would be used for the design of such reefs. To do this, the Babilonia beach of Guardamar del Segura, Alicante (Spain), was chosen because it is a fully anthropised area (with houses in the Maritime-Terrestrial Public Domain, marina, channelling and river mouth) with continuous regression, in which all the elements considered in this study, were treated. Based on the performance obtained in studies and projects worldwide, the climatic characteristics, biocenosis, sediment transport, settlements and liquefaction and the evolution of the coastline, were analysed. The multidisciplinary study carried out showed that with the implementation of a MFAR, the problem was reversed. Furthermore, the area was provided with a playful-economic use, and could be used 60% of the time, by surfers whose skill level were low to intermediate, without forgetting that the diversity of the marine ecosystem in the area was increased. PMID:26851763

  15. Comparative growth and toxin profile of cultured Ostreopsis ovata from the Tyrrhenian and Adriatic Seas.

    Science.gov (United States)

    Guerrini, Franca; Pezzolesi, Laura; Feller, Andrea; Riccardi, Manuela; Ciminiello, Patrizia; Dell'Aversano, Carmela; Tartaglione, Luciana; Dello Iacovo, Emma; Fattorusso, Ernesto; Forino, Martino; Pistocchi, Rossella

    2010-01-01

    Massive blooms of the benthic dinoflagellate Ostreopsis ovata Fukuyo have recently occurred along the whole Italian coastlines, both Tyrrhenian and Adriatic, resulting sometimes in benthonic biocenosis sufferings and, occasionally, in human health problems. In this work, two strains of O. ovata collected in 2006 along the Adriatic and Tyrrhenian coastlines and grown in culture were studied to characterize their growth and toxin profile. The two strains showed different cell volumes, the Adriatic strain being nearly twice bigger than the Tyrrhenian, but they had similar slow growth rates. Liquid chromatography-mass spectrometry (LC-MS) analyses indicated that both strains produce putative palytoxin (pPLTX) and ovatoxin-a (OVTX-a), a palytoxin-like compound presenting 2 oxygen atoms less than palytoxin. Toxin content was determined at the end of the stationary and exponential growth phases and reached the highest value in the Adriatic strain at the end of the stationary phase, with concentrations of 353.3 microg l(-1) for OVTX-a and 30.4 microg l(-1) for pPLTX. Toxin released in the growth medium was also measured and resulted to be the highest at the end of the stationary phase, suggesting that a long lasting bloom could enhance the toxin content in the water and cause toxic effects in people inhaling the aerosol. PMID:19638281

  16. Change of oral microbiocenosis in students with dental caries and chronic catarrhal gingivitis in the dynamics of treatment

    Directory of Open Access Journals (Sweden)

    Shvartsnau E.G.

    2015-11-01

    Full Text Available The violation of microecology plays the important role in the pathogenesis of oral diseases. The question of correction of microecological violations in diseases of hard dental tissues and inflammatory periodontal diseases of young patients is still not enough studied. The aim. This study aims to examine changes of oral microbiocenosis of students with major dental diseases during the treatment. The change in state of oral microbiocenosis has been evaluated in medical students of two age groups: 14-17 years and 18–22 years, in the dynamics of treatment of major dental diseases by developed methods using monotherapy of applications of mucosal gel and combination of mucosal gel and ultraphonophoresis with galaskorbin according to the data which were obtained from the main habitat of the oral cavity: the content of the periodontal groove (gingival fluid, plaque from the vestibular surface of the lower molars, oral fluid. The usage of applications of mucosal gel Kvertgial in students aged 14-22 years removes disbiotic violations and restores normal biocenosis in the oral cavity. Identification of certain microorganisms, estimation of their amount and localization will allow to predict the course of lesions in the oral cavity in terms of causative agent nature and to take preventive measures beforehand with the use of mucosal gel Kvertgial.

  17. [The role of persistence and virulence factors in microecological changes in a humans].

    Science.gov (United States)

    Usviatsov, B Ia; Khusnutdinova, L M; Parshuta, L I; Khanina, E A; Dolgov, V A; Porshina, O V

    2006-01-01

    The patterns of persistence and virulence factors expression in the representatives of human microbial biocenosis depends on a complex of the environmental conditions: influence of microbes-symbionts, biotope peculiarities, properties of microorganisms located within eukaryotes. Interactions of symbionts in pairs "indigen-indigen" isolated from mucous membrane of tonsils in healthy persons, did not lead to changes in expression of pathogenic properties. Interinfluence in pairs "pathogen-indigen" and "indigen-indigen", isolated from patients with chronic tonsilitis were accompanied by an increase of anti-lysozyme, hemolytic and lecithovitellase activities. Migration of strains of non-enzymatized gram-negative bacteria (NEYNB) from nasal into tympanic cavity in experimental acute purulent otitis is connected with an earlier increase of their number in the nasal cavity and the expression of anti-lysozyme activity. In acute and chronic pyoderma, expression of ALA is more marked in bacteria from a perifocal damage in contrast to focal damage of normal skin. In conditions of interaction between erythrocytes and staphylococcal clones with different levels of expression of pathogenic factors, differences were observed in dynamics of hemolytic and anti-hemoglobin activities. PMID:16941873

  18. Benthic foraminifera and trace element distribution: a case-study from the heavily polluted lagoon of Venice (Italy).

    Science.gov (United States)

    Coccioni, Rodolfo; Frontalini, Fabrizio; Marsili, Andrea; Mana, Davide

    2009-01-01

    Living benthic foraminiferal assemblages were studied in surface samples collected from the lagoon of Venice (Italy) in order to investigate the relationship between these sensitive microorganisms and trace element pollution. Geochemical analysis of sediments shows that the lagoon is affected by trace element pollution (Cd, Cu, Ni, Pb, Zn and Hg) with the highest concentrations in its inner part, which corresponds to the Porto Marghera industrial area. The biocenosis are largely dominated by Ammonia tepida, Haynesina germanica and Cribroelphidium oceanensis and, subordinately, by Aubignyna perlucida, Ammonia parkinsoniana and Bolivina striatula. Biotic and abiotic factors were statistically analyzed with multivariate technique of cluster analysis and principal component analysis. The statistical analysis reveals a strong relationship between trace elements (in particular Mn, Pb and Hg) and the occurrence of abnormalities in foraminiferal tests. Remarkably, greater proportions of abnormal specimens are usually found at stations located close to the heaviest polluted industrial zone of Porto Marghera. This paper shows that benthic foraminifera can be used as useful and relatively speedy and inexpensive bio-indicators in monitoring the health quality of the lagoon of Venice. It also provides a basis for future investigations aimed at unraveling the benthic foraminiferal response to human-induced pollution in marine and transitional marine environments. PMID:19733368

  19. Effects of plant downtime on the microbial community composition in the highly saline brine of a geothermal plant in the North German Basin.

    Science.gov (United States)

    Westphal, Anke; Lerm, Stephanie; Miethling-Graff, Rona; Seibt, Andrea; Wolfgramm, Markus; Würdemann, Hilke

    2016-04-01

    The microbial biocenosis in highly saline fluids produced from the cold well of a deep geothermal heat store located in the North German Basin was characterized during regular plant operation and immediately after plant downtime phases. Genetic fingerprinting revealed the dominance of sulfate-reducing bacteria (SRB) and fermentative Halanaerobiaceae during regular plant operation, whereas after shutdown phases, sequences of sulfur-oxidizing bacteria (SOB) were also detected. The detection of SOB indicated oxygen ingress into the well during the downtime phase. High 16S ribosomal RNA (rRNA) and dsrA gene copy numbers at the beginning of the restart process showed an enrichment of bacteria, SRB, and SOB during stagnant conditions consistent with higher concentrations of dissolved organic carbon (DOC), sulfate, and hydrogen sulfide in the produced fluids. The interaction of SRB and SOB during plant downtimes might have enhanced the corrosion processes occurring in the well. It was shown that scale content of fluids was significantly increased after stagnant phases. Moreover, the sulfur isotopic signature of the mineral scales indicated microbial influence on scale formation. PMID:26610802

  20. [Parasites as a natural element of any ecosystem].

    Science.gov (United States)

    Pojmańska, Teresa

    2002-01-01

    The article presents some ideas upon the functioning of the parasite populations and communities in relation to the whole ecosystem. The author underlines that the parasites are one of the natural elements of every biocenosis and their existence submittes to similar ecological rules as the populations and communities of free-living organisms. Colonization of their particular environment (living organism) demands some adaptations allowing to resist immunological defense of the host, and to coexist with other parasite species harboured by the same host, not exploiting the supplies over the host "carying capacity", what could provoke the strong pathogenic effects, including host death. The mechanims maintaning the state of relative stability in host-parasite system are similar as in the case of free-living organisms, and are of both parasite- and host origin. They can confine the number of parasites (elimination of some specimens) or their biomass (growth restraint), limit their fecundity (maturation restraint), or make particular species to use different supply (niches separation). On the other hand parasite can regulate the demography of host population: restrict their fecundity, or kill their host directly or indirectly (making them an easier prey for predators). The author presents and discusses some examples illustrating the activity of the regulating mechanisms in host-parasite relationship, as well as the role of parasites in energetic ballance of ecosystem and in "engineering of ecosystem". PMID:16888938

  1. Biogas production from mono-digestion of maize silage-long-term process stability and requirements.

    Science.gov (United States)

    Lebuhn, M; Liu, F; Heuwinkel, H; Gronauer, A

    2008-01-01

    Biogas production from mono-digestion of maize silage was studied for more than one year in six continuously stirred, daily fed 36 L fermenters. Chemical and microbiological parameters were analysed concomitantly. The reactors acidified already after 8 months of operation at a low organic loading rate (OLR) of 2 g VS*(L*d)(-1). The TVA/TAC ratio was the most reliable parameter to indicate early process instabilities leading to acidification. A TVA/TAC threshold of 0.5 should not be exceeded. After acidification and recovery of the fermenters, propionic acid was no reliable parameter anymore to indicate process failure, since values far below the threshold of 1 g*L(-1) were obtained although the process had collapsed.The acidified reactors recovered better, showed greatly improved stability and allowed a higher OLR when a trace element (TE) cocktail was supplemented. Hydrolysis was obviously not process-limiting, results indicated that methanogens were affected. The most limiting element in long-term mono-digestion of maize silage turned out to be cobalt, but data obtained suggest that molybdenum and selenium should also be provided. TE supplementation should be designed specifically in order to meet the actual needs. TE availability for the biocenosis appears to be a key issue in biogas production, not only in mono-but also in co-digestion processes. PMID:19001720

  2. Limnological survey in eight high mountain lakes located in Lago Maggiore watershed (Switzerland

    Directory of Open Access Journals (Sweden)

    Viera STRAŠKRABOVÁ

    1999-08-01

    Full Text Available Eight high mountain lakes located above 2000 m a.s.l. in the watershed of Lago Maggiore, with alkalinity between 10 and 100 μeq l-1, were studied during summer 1997. The survey revealed that some of the lakes had a saline density gradient which might hinder the mixing of water and restrict the oxygenation in hypolimnion. Following acidification, aluminium was present in some lakes in the form of Al(OH2 +, with pH around 5.9, and as Al(OH4 -, with pH around 6.6. The negative correlation observed between pH and nitrate concentrations seems to be linked to vegetal activity. In fact, acidity in soil and water, combined with the presence of aluminium, can partially inhibit the metabolism of plants and algae, and reduce the assimilation of NO3 -. Major biological differences emerged among the lakes, both at the level of plankton composition and biomass, and in seasonal dynamics. Dinophyceans and chlorophyceans predominated quantitatively in the lakes with low pH and alkalinity values, whereas diatoms were present in the lakes with higher values of these parameters. The phytoplankton biodiversity index showed a considerable variation from lake to lake. Higher alkalinity and salinity was not coupled to a higher biocenosis diversity and one or two species tend to dominate the others.

  3. Remobilisation of uranium from contaminated freshwater sediments by bioturbation

    Directory of Open Access Journals (Sweden)

    S. Lagauzère

    2013-10-01

    Full Text Available Previous studies have demonstrated that benthic macro-invertebrate bioturbation can influence the remobilization of uranium initially associated with freshwater sediments resulting in a high release of this pollutant through the overlying water column. Giving the potential negative effects on aquatic biocenosis and the global ecological risk, it appeared crucial to improve our current knowledge concerning the uranium biogeochemical behaviour in sediments. The present study aimed to assess the biogeochemical modifications induced by Tubifex tubifex (Annelida, Clitellata, Tubificidae bioturbation within the sediment permitting to explain such a release of uranium. To reach this goal, uranium distribution between solid and solute phases of a reconstructed benthic system (i.e. in mesocosms inhabited or not by T. tubifex worms was assessed in a 12 day laboratory experiment. Thanks notably to fine resolution (mm-scale measurements (e.g. DET gels probes for porewater, bioaccumulation in worms of uranium and main chemical species (iron, sulfate, nitrate, nitrite, this work permitted (i to confirm that the removal of bottom sediment particles to the surface through the digestive tract of worms greatly favours the oxidative loss of uranium in the water column, and (ii to demonstrate that both uranium contamination and bioturbation of T. tubifex substantially influence major microbial-driven biogeochemical reactions in sediments (e.g. stimulation of denitrification, sulfate-reduction and iron dissolutive reduction. This study provides the first demonstration of biogeochemical modifications induced by bioturbation in freshwater uranium-contaminated sediments.

  4. Recreational Boating in Ligurian Marine Protected Areas (Italy): A Quantitative Evaluation for a Sustainable Management.

    Science.gov (United States)

    Venturini, S; Massa, F; Castellano, M; Costa, S; Lavarello, I; Olivari, E; Povero, P

    2016-01-01

    Recreational boating is an important economic activity that can also represent a powerful source of interference for biological communities. The monitoring of the recreational boating in all Marine Protected Areas (MPAs) within the Liguria region was conducted in the 2010 summer season and it allowed to obtain information not provided by any official institution. The collaboration of geographically different MPAs in Liguria has led to the implementation of a monitoring framework of recreational boating, and this has made it possible to develop uniform management strategies for all the Ligurian marine parks. This study identifies the optimal number of boats for each MPAs, the number of boats that can anchor in the various parks without creating any impact on the biocenosis of merit, providing a first characterization of recreational boating in Liguria during the high touristic season and providing management recommendation to each MPAs. Generally, the Ligurian MPAs do not present critical situations, the number of boats in each MPA being below the optimal number, with the exception of Portofino MPA, where in the 12.5 % of monitored days more than 220 boats were counted and the mean density for weekend is 1.19 no boats/ha (4 times higher than weekday). The results confirm the dependence of the boats peaking from the holidays and the months of the summer, but also it highlights other factors that can contribute in the choice of the boaters. PMID:26289349

  5. Bacterial degradation of naproxen--undisclosed pollutant in the environment.

    Science.gov (United States)

    Wojcieszyńska, Danuta; Domaradzka, Dorota; Hupert-Kocurek, Katarzyna; Guzik, Urszula

    2014-12-01

    The presence of non-steroidal anti-inflammatory drugs (NSAIDs) in the environment is an emerging problem due to their potential influence on human health and biocenosis. This is the first report on the biotransformation of naproxen, a polycyclic NSAID, by a bacterial strain. Stenotrophomonas maltophilia KB2 transformed naproxen within 35 days with about 28% degradation efficiency. Under cometabolic conditions with glucose or phenol as a carbon source degradation efficiency was 78% and 40%, respectively. Moreover, in the presence of naproxen phenol monooxygenase, naphthalene dioxygenase, hydroxyquinol 1,2-dioxygenase and gentisate 1,2-dioxygenase were induced. This suggests that degradation of naproxen occurs by its hydroxylation to 5,7,8-trihydroxynaproxen, an intermediate that can be cleaved by hydroxyquinol 1,2-dioxygenase. The cleavage product is probably further oxidatively cleaved by gentisate 1,2-dioxygenase. The obtained results provide the basis for the use of cometabolic systems in the bioremediation of polycyclic NSAID-contaminated environments. PMID:25026371

  6. The potential of use the recyclable organic materials for soil fertilization

    Directory of Open Access Journals (Sweden)

    Florica Morariu

    2014-05-01

    Full Text Available The decrease productivity of soils that occurs following an agricultural overproduction is manifested by insufficient of nutrients, nitrogen and phosphorus, by salinity increasing, humus deficiency, soil aggregation changing moisture content modified, etc, finally through undesirable phenomena of erosion. The uses of sewage sludge for fertilizing purposes of degraded soils remain a method of managing this recyclable waste. Sewage sludge anaerobically stabilized brings into soil an important input of nutrients (C, N, P, micronutrients and an significant settlement of compatible microorganisms with soil biocenosis. The potential hazards of sewage sludge due to pathogenic agents load is reduced by anaerobic stabilization. The potential of physicochemical contaminants is controlled by national legislation. Using a quantity of 40 t·ha-1 sewage sludge anaerobically stabilized for fertilization of cultivated soils with maize led to an increase of grain production of 12 % over the amount harvested from a control field and with 5 % of the amount collected from a fertilized field with 40 t·ha-1 manure. In the aerial parts of the harvested plants from fertilized fields with the sewage sludge haven’t accumulated toxic metals (Cd, Ni, Cr, Pb in the detectable range, and the amount of Zn was according to the nutritional requirements.

  7. Revisiting dandruff.

    Science.gov (United States)

    Piérard-Franchimont, C; Xhauflaire-Uhoda, E; Piérard, G E

    2006-10-01

    Dandruff is a common scalp disorder affecting almost half of the postpubertal population of any ethnicity and both genders. It may, however, represent a stubborn esthetical disturbance often source of pruritus. Skin biocenosis, in particular the Malassezia spp. flora, plays a key aetiologic role, in combination with the unusual capacity of some corneocytes to be coated by these yeasts. Substantial evidence indicates that keratinocytes play an active role in the generation and expression of immunopathological reactions. This is probably the case in dandruff. Upon stimulation of a critical colonization of corneocytes by Malassezia yeasts, the release of pro-inflammatory mediators is increased. This could lead to the subclinical microinflammation present in dandruff. In seborrheic dermatitis, local deposits of immunoglobulins and the release of lymphokines are responsible for the recruitment and local activation of leukocytes leading to the eventual amplification of the inflammatory reaction. Some ancillary non-microbial causes of dandruff may operate through physical or chemical irritants. Many methods have been described for rating dandruff. Our favourite tools are clinical examination and squamometry. Dandruff can precipitate telogen effluvium and exacerbate androgenic alopecia. Antidandruff formulations exhibiting some direct or indirect anti-inflammatory activity can improve both dandruff and its subsequent hair cycle disturbance. PMID:18489295

  8. [Modification of antibiotic resistance in microbial symbiosis].

    Science.gov (United States)

    Aznabaeva, L M; Usviatsov, B Ia; Bukharin, O V

    2010-01-01

    In antibiotic therapy it is necessary to use drugs active against the pathogen in its association with the host normal microflora. The aim of the study was to investigate modification of antibiotic resistance under conditions of the pathogen association with the representatives of the host normal microflora and to develop the microbiological criteria for determining effectiveness of antibacterials. Modification of microbial antibiotic resistance was investigated in 408 associations. Various changes in the antibiotic resistance of the strains were revealed: synergism, antagonism and indifference. On the basis of the results it was concluded that in the choice of the antibiotic active against Staphylococcus aureus and Streptococcus pyogenes the preference should be given to oxacillin, gentamicin and levomycetin, since the resistance of the pathogens to these antibiotics under the association conditions did not increase, which could contribute to their destruction, whereas the resistance of the normoflora increased or did not change, which was important for its retention in the biocenosis. The data on changeability of the antibiotic resistance of the microbial strains under the association conditions made it possible to develop microbiological criteria for determining effectiveness of antibiotics in the treatment of inflammatory diseases of microbial etiology (RF Patent No. 2231554). PMID:21033469

  9. Microbial metabolism in oil fields and production plants. Final report. Mikrobielle Stoffwechselprozesse in Erdoelfeldern und Gewinnungsanlagen. Abschlussbericht

    Energy Technology Data Exchange (ETDEWEB)

    Naeveke, R.; Graff, M.

    1987-04-01

    In laboratory experiments and by attempts to produce enrichment cultures of bacteria from samples of oil deposits the influence of the ecological parameters salt concentration, temperature and hydrostatic pressure on the establishment of microbial dissimilatory sulfate reduction were looked into. In situ sulfate reduction in untampered deposits would appear to be very unlikely. But through the influence of secondary or tertiary operations (even in deep deposits) bacterial H/sub 2/S production may be initiated. In the type of oil deposit investigated the participation of an autochthonous bacterial biocenosis can almost certainly be ruled out. On the question of the availability of energy and nutrient sources and of the terminal electron acceptor sulfate, it could be shown that most deposits contained sufficient ammonium and sulfate to support dissimilatory sulfate reduction, while phosphate appeared often to be a limiting factor. In addition it is shown that the degradation of components from natural deposits is of no significance. Some oil field chemicals, on the other hand, provide good sources of energy and nutrients at whose expense, through a syntrophism between sulfate reducing and non sulfate reducing bacteria, dissimilatory sulfate reduction can occur. Other oil field chemicals may provide sulfate or phosphate. The effects of these circumstances on practical operations are discussed. With 9 figs., 39 tabs..

  10. [Influence of the phase of the number and demographic structure of the water vole population on its infection by helminthes].

    Science.gov (United States)

    Chechulin, A I; Guliaev, V D; Panov, V V; Krivopalov, A V

    2005-01-01

    In this work we have analyzed results of the long-term investigations of the helminthes distribution in the various demography groups of the water vole population (Arvicola terrestris L.) in North Baraba (Novosibirsk region). The data on the dominant parasites of these rodents: trematodes Notocotylus noyeri (Joyeux, 1922), cestodes Limnolepis transfuga Spassky et Merkuscheva, 1967, nematodes Capillaria wioletti Ruchljadeva, 1950, Longistriata minuta (Dujarden, 1845) and Heligmosomum costellatum (Dujarden, 1845) have shown that the number of parasites in biocenosis are connected with different factors, such as the demographic structure of the host population, the alternation of hosts number and conditions of the environment (dry and damp phases of the climatic cycle). In the dry phase the main parasitize load N. noyeri, L. transfuga and C. wioletti connects with the breeding group; in the damp period - with immature rodents. Independently of the phase climatic cycle and the density of the water vole population the great bulk of nematodes L. minuta and H. costellatum was uncovered of the immature individuals. In any case the number of helminthes changed synchronously with such of the its host. PMID:16316057

  11. Role of bacteria in the removal of benzopyrene from waste water in the coke by-products industry

    Energy Technology Data Exchange (ETDEWEB)

    Yurovskaya, E.M.

    1985-12-01

    Role of specific microflora is studied (the phenol destroying pseudomonad Pseudomonas aeruginosa and a rare strain of thionic bacteria Thiobacillus thiocyanoxidans which breaks down thiocyanates), in biochemical purification installations for removing benzopyrene from coal-tar effluent. Tests are described made in synthetic media with phenol and thiocyanate concentrations of 200 mg/l and 50 mg/l respectively, in waste water from coking plants and in ordinary industrial effluent, in order to study their resistance to benzopyrene and the capacity for intracellular accumulation and biochemical transformation as well as distribution in the biocenosis of purifying installations. The tests were also carried out in elective synthetic media with benzopyrene concentrations varying from 10-200 ..mu..g/l to discover a possible role of phenol destroying pseudomonad and thionic bacteria in decarcinogenization of waste water. The data obtained make it possible to assume that the widely used microbiological method for removing dangerous ingredients (phenols, thiocyanates, cyanide) from waste water will also help to reduce the amount of benzopyrene carcinogenic compound by approximately 49.4-56.04%. 11 references.

  12. VAGINAL MICROECOLOGY IN WOMEN WITH METABOLIC SYNDROME IN CLIMACTERIC PERIOD

    Directory of Open Access Journals (Sweden)

    Berihanova R. R.

    2016-03-01

    Full Text Available The aim of the research is to study the peculiarities of vaginal microbiocenosis in female patients with metabolic syndrome during peri- and postmenopause. 320 women in the climacteric period aged of 45-70 were examined. Two groups were formed: the main group (160 female patients with metabolic syndrome, the control group (160 women without metabolic syndrome. The average age of women 54.5 ± 7.2 years. Depending on the length of menopause each of the two groups was divided into three subgroups.Microscopy of vaginal smears, the colpocytological analysis, the culture test of vaginal discharge were conducted. The statistical processing of the results was carried out with the help of programme sets Microsoft Office 2010 (MicrosoftExcel and «STATISTICA® for Windows 6.0». A tendency of decreasing frequency of non-specific vaginitis with aging and of increasing frequency of vaginal atrophy, disorders of vaginal biocenosis (lactobacillus deficiency and the domination of conditionally pathogenic microorganisms was discovered. The frequency of vaginal atrophy in women with a length of postmenopause of 10 years and older was 65.9% in the subgroup of in female patients with metabolic syndrome and 63.6% in the subgroup of female patients patients without it. The more observable changes in vaginal biotope were found in women with metabolic syndrome

  13. STUDY UPON THE SPECIES IPS TYPOGRAPHUS L. (COLEOPTERA, CURCULIONIDAE IN THE RAŞINARI FORESTRY ECOSYSTEM, SIBIU COUNTY

    Directory of Open Access Journals (Sweden)

    Iuliana ANTONIE

    2015-04-01

    Full Text Available The disturbance of the equilibrium between the endogenous and exogenous command factors of human origin leads to massive perturbations in the forestry ecosystems affecting all the living beings within the biocenosis and especially the forestry entomologic fauna. Under some circumstances the pest insects from the forestry ecosystem can produce big damages to the trees in the case of maintaining high effectives of these and for many years, too. Our study aimed the monitoring the species Ips typographus L., a forestry pest, which by its action produces important damages to the spruce fir. The research work ran for two years, during 2012-2013, in the area of Raşinari Forestry District, Sibiu County. The work method was to install at the skirt of the forest the traps with pheromones bait in the researched area. There were collected a number of 4,146 samples of which in 2012 were captured 1,973 individuals and in 2013 were captured 2,173 individuals. There was established a growth of the pest population in the studied biotope, this being the same as at the national level. As a consequence there are imposed further studies in order to find the most proper solutions regarding stopping the dissemination of the insect into new territories and maintaining the density of the population of the pest beyond the economic level of damage.

  14. Mesophilic anaerobic stabilization of sewage sludge. Mesophile anaerobe Klaerschlammstabilisierung mit aerober Folgebehandlung

    Energy Technology Data Exchange (ETDEWEB)

    Stoll, U.

    1988-01-01

    Sludges treated in two stages in experiments - 7 days of anaerobic treatment and 2 days of aerobic-thermophilic treatment - can be judged to be completely stabilized because of the stabilization parameters BOD/sub 5//COD ratio and respiratory activity. The degradation results obtained are comparable to or better than those of the 20-day digestion (reference process). For all aerobic processes under investigation a clear temperature increase in the aerobic reactor was measured because of the exothermal metabolic processes of the aerobic biocenosis. There was a temperature rise of 15/sup 0/C in the tests in the aerobic reactor even after longer digestion times of 15 and 20 days. The results of the epidemics and hygiene investigations show that a secondary aerobic-thermophilic stage after the mesophilic digestion with adequate marginal conditions - germ retention time of 23 hours in the aerobic reactor at process temperatures higher than 50/sup 0/C as well as charging in batch quantities - leads to a safe and complete decontamination. Under these process and operation conditions all salmonellae were killed and the number of the enterobacteriaceae in 1 g of sludge was always less than 1.000. (orig./EF).

  15. Genetic 'fingerprints' to characterise microbial communities during organic overloading and in large-scale biogas plants

    Energy Technology Data Exchange (ETDEWEB)

    Kleyboecker, A.; Lerm, S.; Vieth, A.; Wuerdemann, H. [GeoForschungsZentrum Potsdam, Bio-Geo-Engineering, Potsdam (Germany); Miethling-Graff, R. [Bundesforschungsanstalt fuer Landwirtschaft, Braunschweig (Germany). Inst. fuer Agraroekologie; Wittmaier, M. [Institut fuer Kreislaufwirtschaft, Bremen (Germany)

    2007-07-01

    Since fermentation is a complex process, biogas reactors are still known as 'black boxes'. Mostly they are not run at their maximum loading rate due to the possible failure in the process by organic overloading. This means that there are still unused capacities to produce more biogas in less time. Investigations of different large-scale biogas plants showed that fermenters are operated containing different amounts of volatile fatty acids. These amounts can vary so much that one of two digestors, both possessing the same VFA concentration, does not produce gas anymore while the other is still at work. A reason for this phenomenon might be found in the composition of the microbial communities or in differences in the operation of the plants. To gain a better understanding of the 'black box', structural changes in microbial communities during controlled organic overloading in a laboratory and biocenosis of large-scale reactors were investigated. A genetic fingerprint based on 16S rDNA (PCR-SSCP) was used to characterise the microbial community. (orig.)

  16. Polishing domestic wastewater on a subsurface flow constructed wetland: organic matter removal and microbial monitoring.

    Science.gov (United States)

    Aguiar-Pinto Mina, I; Costa, M; Matos, A; Sousa Coutinho Calheiros, C; Castro, P M L

    2011-01-01

    Microbial monitoring of constructed wetlands (CWs) treating domestic wastewater is generally scarce, despite the need of more knowledge about its biocenosis. The sanitation quality of a wastewater treated in a CW is a crucial aspect, mainly when the receiving water body is used as a swimming and/or recreation area. The present study was carried out in a horizontal subsurface flow CWplanted with Phragmites australis receiving pre-treated domestic wastewater (mean flow 50 m3 day(-1)), from a population of about 300 inhabitants. The monitoring programme undertaken during the first year operation, revealed removal efficiencies of 61% BOD5, 44% COD, and 65% TSS for inlet water with ca. 90 mg L(-1) BOD5, 157 mg L(-1) COD, and 17 mg L(-1) TSS. Total Coliform (TC) and Faecal Coliform (FC) bacteria were removed from wastewater (mean inlet values of 5 x 10(6) CFU 100 mL(-1) TC and of 9 x 10(5) CFU 100 mL(-1) FC), with efficiencies of 92 and 97%, respectively. The dynamics of microbial communities established in the system assessed by polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE), had revealed a high bacterial diversity within the system, with no relevant differences in composition at the CW inlet and outlet but exhibiting temporal differences in bacterial communities. PMID:21972563

  17. Isotope effects for carbon and hydrogen with bacterial oxidation of oils and certain petroleum hydrocarbons

    Energy Technology Data Exchange (ETDEWEB)

    Mekhtiyeva, V.L.; Guriyeva, S.M.; Kondrat' yeva, G.F.

    1983-01-01

    Results are presented of experiments on bacterial oxidation of oils, decane and hexadecane. Oxidation of oils was done by natural biocenosis isolated from bed waters, hydrocarbons by pure culture of bacteria from the river Pseudomonas. As a result of bacterial transformation, the methane oils were enriched with the isotope C/sup 12/, the methane-naphthene by isotope C/sup 13/. The carbon dioxide formed during oxidation of oils was fairly close in deltaC/sup 13/ values to the original oils. The oils exposed to biodegradation in aerobic conditions were enriched with deuterium, in anaerobic--they were slightly depleted. With oxidation of individual hydrocarbons, the isotope effects for carbon were extremely small, and for hydrogen were missing altogether. As a result of analysis of the presented data, as well as the previously published materials, conclusions were drawn about the nature of the isotope effects with bacterial oxidation of the oils and scales were defined with different degree of oxidation of the oils.

  18. Improving effective preparation of well products in West Siberia. Povysheniye effektivnosti podgotovki produktsii skvazhin v Zapadnoy Sibiri

    Energy Technology Data Exchange (ETDEWEB)

    1984-01-01

    Operation of West Siberian fields is characterized in the present period by significant complication in the conditions for extracting and preparation of oil. This is due to the mass transition of the extracting fund to mechanized methods and sharp rise in the degree of well product flooding. An analysis is made of the features of extraction and preparation of oil in new, complicated conditions and methods are presented for improving the efficiency of these processes. A number of solutions are suggested developed by the Siberian scientific research association of the oil industry: method of separating oil with high gas factor; intensification of degasification of oil at the terminal separation units; technology of oil de-emulsification with the use of de-emulsifying reagents; technology of improving well productivity by modifying the near-face bed zone with high, multiple depressions, etc. Attention is focused on the complications in oil extraction such as the formation of hydrate plugs in the gas-lift wells, depositing of inorganic salts in the pumping equipment and manifestation of biocenosis.

  19. [GynOphilus--a winning relay].

    Science.gov (United States)

    Kovachev, S

    2012-01-01

    The vagina is a complex biocenosis where many micro-organisms coexist and colonize it. The dominant colonizing bacteria of a healthy individual is of the genus "lactobacillus". It is also called Doderlein's bacillus and determines the vaginal microbial balance through the production of lactic acid, hydrogen peroxide, biosurfactants, bacteriocines and modify the competition of pathogens for adhesion to the vaginal epithelial cells. Through these mechanisms the lactobacilli block the growth and development of other vaginal pathogenic microbial species and also inhibit the colonization of some other microorganisms imported from outside. Because of these potential therapeutic properties, the lactobacilli are used as effective medical agents for prophylaxys and therapy to restore the physiological balance in the vaginal eco-system. According to our studies and gained clinical experience the etiological antibacterial treatment is not always sufficient to restore the normal vaginal flora. The complete recovery of the vaginal flora could be reached using probiotics that are applied locally and per os. GynOphilus is a new vaginal probiotic product containing one type of the genus "Lactobacillus": Lactobacillus casei var rhamnosus. GynOphilus restores the physiological balance of the vaginal flora and reduces the risk of recurrent infection. The product is applied intravaginal and interacts locally, inhibits the growth of the most common vaginal pathogens: Gardnerella vaginalis u Candida albicans. PMID:23236678

  20. Recreational Boating in Ligurian Marine Protected Areas (Italy): A Quantitative Evaluation for a Sustainable Management

    Science.gov (United States)

    Venturini, S.; Massa, F.; Castellano, M.; Costa, S.; Lavarello, I.; Olivari, E.; Povero, P.

    2016-01-01

    Recreational boating is an important economic activity that can also represent a powerful source of interference for biological communities. The monitoring of the recreational boating in all Marine Protected Areas (MPAs) within the Liguria region was conducted in the 2010 summer season and it allowed to obtain information not provided by any official institution. The collaboration of geographically different MPAs in Liguria has led to the implementation of a monitoring framework of recreational boating, and this has made it possible to develop uniform management strategies for all the Ligurian marine parks. This study identifies the optimal number of boats for each MPAs, the number of boats that can anchor in the various parks without creating any impact on the biocenosis of merit, providing a first characterization of recreational boating in Liguria during the high touristic season and providing management recommendation to each MPAs. Generally, the Ligurian MPAs do not present critical situations, the number of boats in each MPA being below the optimal number, with the exception of Portofino MPA, where in the 12.5 % of monitored days more than 220 boats were counted and the mean density for weekend is 1.19 no boats/ha (4 times higher than weekday). The results confirm the dependence of the boats peaking from the holidays and the months of the summer, but also it highlights other factors that can contribute in the choice of the boaters.

  1. Functional-environmental assessment of Chernozems' technogenic changes in the Central-Chernozem Region of Russia

    Science.gov (United States)

    Deviatova, Tatiana; Jablonskikh, Lidiia; Alaeva, Liliia; Negrobova, Elena

    2015-04-01

    Long-term research revealed the significant changes in Chernozems of the recreational, urban, industrial and transport areas in the eastern part of the Central-Chernozem Region of Russia. They are reflected in the soil properties and regimes disturbances that determine their environmental functions. The level of the Chernozems' anthropogenic degradation is usually determined not only by their pollutants composition and quantity but by landscape, microclimatic, hydrological and soil features too. The Chernozems strongest degradation has been observed in the industrial zone and central part of Voronezh, and also in the 20-m zone of the highway "Don" with maximum technogenic input intensity and depth. The Chernozems' resistance to contamination is determined by their texture, organic matter content and quality, microbial activity and biochemical processes, pH and redox power. The level of the pollution impact on the organic substances decomposition can be evaluated according to the extracellular biological processes changes from their standard rates: 50% - very hazardous (ecotoxicological scale). The investigated soil types and subtypes have essentially different resistance to their contamination. In case of the gray forest soils already medium input of pollutants often results in irreversible changes in their biocenosis functions. In case of the leached Chernozems 50%-drop in their biological state occurs only at high levels of pollution. The developed criteria reflect the man-made ecosystems' soil principal changes and can be useful in prediction of their environmental functions.

  2. Chemical and biological monitoring of MIOR on the pilot area of Vyngapour oil field, West Sibera, Russia

    Energy Technology Data Exchange (ETDEWEB)

    Arinbasarov, M.U.; Murygina, V.P.; Mats, A.A.

    1995-12-31

    The pilot area of the Vyngapour oil field allotted for MIOR tests contains three injection and three producing wells. These wells were treated in summer 1993 and 1994. Before, during, and after MIOR treatments on the pilot area the chemical compounds of injected and formation waters were studied, as well as the amount and species of microorganisms entering the stratum with the injected water and indigenous bacteria presented in bottomhole zones of the wells. The results of monitoring showed that the bottomhole zone of the injection well already had biocenosis of heterotrophic, hydrocarbon-oxidizing, methanogenic, and sulfate-reducing bacteria, which were besides permanently introduced into the reservoir during the usual waterflooding. The nutritious composition activated vital functions of all bacterial species presented in the bottomhole zone of the injection well. The formation waters from producing wells showed the increase of the content of nitrate, sulfate, phosphate, and bicarbonate ions by the end of MIOR. The amount of hydrocarbon-oxidizing bacteria in formation waters of producing wells increased by one order. The chemical and biological monitoring revealed the activation of the formation microorganisms, but no transport of food industry waste bacteria through the formation from injection to producing wells was found.

  3. Double stage dry-wet-fermentation - start-up of a pilot biogas plant

    International Nuclear Information System (INIS)

    The Brandenburg University of Technology (BTU) has developed a double stage dry-wet fermentation process for fast and safe anaerobic degradation. Originally designed for treatment of organic wastes, this process allows using a wide variety of solid biodegradable materials. The dividing of hydrolysis and methanation in this process, allows an optimization of the different steps of biogas generation separately. The main advantages of the process are the optimum process control, an extremely stable process operation and a high gas productivity and quality. Compared to conventional processes, the retention times within the percolation stage (hydrolysis) are reduced considerably. In cooperation with the engineering and consulting company GICON, the technology was qualified further to an industrial scale. In 2007 a pilot plant, and, simultaneously, an industrial plant were built by GICON based on this double stage technology. Based on practical experience from the operation of laboratory fermentation plants, the commissioning of the pilot plant was planned, controlled and monitored by our institution. The start-up of a biogas plant of this type focuses mainly on the inoculation the of methane reactor. The growth of microbial populations and generation of a stable biocenosis within the methane reactor is essential and affects the duration of starting period as well as the methanation efficiency a long time afterwards. This paper concerns with start-up of a pilot biogas plant and discusses particular occurrences and effects during this period. (author)

  4. Remobilisation of uranium from contaminated freshwater sediments by bioturbation

    Science.gov (United States)

    Lagauzère, S.; Motelica-Heino, M.; Viollier, E.; Stora, G.; Bonzom, J. M.

    2014-06-01

    Benthic macro-invertebrate bioturbation can influence the remobilisation of uranium (U) initially associated with freshwater sediments, resulting in a high release of this pollutant through the overlying water column. Given the potential negative effects on aquatic biocenosis and the global ecological risk, it appears crucial to improve our current knowledge concerning the biogeochemical behaviour of U in sediments. The present study aimed to assess the biogeochemical modifications induced by Tubifex tubifex (Annelida, Clitellata, Tubificidae) bioturbation within the sediment in order to explain such a release of U. To reach this goal, U distribution between solid and solute phases of a reconstructed benthic system (i.e. in mesocosms) inhabited or not by T. tubifex worms was assessed in a 12-day laboratory experiment. Thanks notably to fine-resolution (mm-scale) measurements (e.g. "diffusive equilibrium in thin-films" DET gel probes for porewater, bioaccumulation in worms) of U and main chemical species (iron, sulfate, nitrate and nitrite), this work (i) confirmed that the removal of bottom sediment particles to the surface through the digestive tract of worms greatly favoured oxidative loss of U in the water column, and (ii) demonstrated that both U contamination and bioturbation of T. tubifex substantially influenced major microbial-driven biogeochemical reactions in sediments (e.g. stimulation of denitrification, sulfate reduction and iron dissolutive reduction). This study provides the first demonstration of biogeochemical modifications induced by bioturbation in freshwater U-contaminated sediments.

  5. El modelo de zonación en la orilla rocosa del departamento de Lima

    Directory of Open Access Journals (Sweden)

    Carlos Paredes Q.

    2014-06-01

    Full Text Available Durante el año 1972 y parte de 1973 se estudió la zonación biocenológica en la orilla rocosa maciza del Departamento de Lima, entre los 10°45'38"S y los 12°57'39"S, con la finalidad de establecer el esquema de zonación aplicable, entre los diversos esquemas empleados en otros lugares del mundo. En siete lugares guía, se delimitaron los niveles de pleamar y bajamar de sicigias y cuadraturas, para determinar la distribución vertical de los organismos, asociaciones y comunidades que integran la biocenosis de este tipo de orilla, caracterizando los diversos niveles de la zona entre mareas. Se encontró aplicable el siguiente esquema zonacional, modificado de Stephenson y Stephenson (1949: La ZONA LITORAL se extiende desde el nivel superior de Littorina peruviana, hasta el nivel promedio de las bajamares de sicigias ordinarias (MLWS = 0 m, y comprende: a Franja supralitoral, extendida entre el nivel superior de Littarina peruviana y el límite superior de Chthamalus cirratus. b Zona mediolitoral, extendida desde el límite inferior de la franja supralitoral hasta el nivel superior de Megabalanus psittacus; y la Franja infralitorol, comprendida desde el nivel inferior de la zona mediolitoral hasta la línea de bajamares medias de sicigias ordinarias.

  6. Microbiological monitoring of carbon dioxide storage in a subsurface saline aquifer in Ketzin/Germany within the scope of CO2SINK

    Science.gov (United States)

    Wandrey, M.; Morozova, D.; Zemke, K.; Lerm, S.; Scherf, A.-K.; Vieth, A.; Würdemann, H.; Co2SINK Group

    2009-04-01

    Within the scope of the EU project CO2SINK (www.co2sink.org) a research facility in Ketzin (Germany, west of Berlin) is operated to store CO2 in a saline subsurface aquifer (Würdemann et al., EGU General Assembly 2009). In order to examine the influence of CO2 storage on the environment a comprehensive monitoring program is applied at this site including molecular and microbiological investigations. With the injection of CO2 into the geological formation chemical and physical reservoir characteristics are changed. This may influence the composition and activities of the deep biosphere at the storage horizon. Mineral precipitation, dissolution and corrosion of reservoir casing may be consequences, influencing permeability and long-term stability of the reservoir. The objective of the microbial monitoring program is the characterisation of the microbial community (biocenosis) in fluid samples, as well as in samples from reservoir and cap rock before and during CO2storage using molecular biological methods. 16S rRNA taxonomic studies, Fluorescence in situ hybridisation (FISH), and RealTime PCR are used to examine the composition of the biocenosis. First results of fluid sampling revealed that the microbial community of the saline aquifer is dominated by haloalkaliphilic fermentative bacteria and extremophilic organisms, coinciding with reduced conditions, high salinity and pressure. RealTime RT-PCR of selected genes and the creation and analysis of cDNA libraries will allow the prediction of microbial metabolic activities. In addition, the analysis of organic and inorganic components of the samples will add to the knowledge of possible metabolic shifts during CO2 storage. In order to simulate the storage conditions in situ, long term laboratory experiments in high pressure incubators have been set up using original rock cores from Ketzin. Since DNA and RNA analysis techniques are very sensitive, contamination entries from the adjacent environment have to be excluded

  7. Ecología de ostracodos en medios estuarinos: el Subsistema Carreras (río Guadiana, SO de España

    Directory of Open Access Journals (Sweden)

    Morales, J. A.

    1997-12-01

    Full Text Available Analyses from 69 samples collected in the Carreras subsystem delimited ten grain size distributions (five in the channel, four in the channel border and one in the salt marsh. The geochemical analysis of some samples shows low pollution in this zone. Four ostracod assemblages were delimitated: a indigenous estuarine assemblage (Loxoconcha elliptica, Cyprideis torosa, Cytherois fischeri, Elofmnia pusilla and Leptocythere sp., dominant in all samples, both in biocoenosis and tanathocoenosis; b coastal assemblage (Urocythereis oblonga, Pontocythere elongata, Aurila convexa and Cytheretta adriatica, with high species well represented in the main channels with marine connection; c open sea assemblage (Carinocythereis whitei, Palmoconcha guttata and Palmoconcha turbida, with small species very abundant in deeper marine environments (> 10 m deep of the Huelva littoral; and d fresh water assemblage (Candona sp., Cyprinotus salinus and Limnocythere inopinata, very minoritary in the silty-muddy tidal flats. The estuarine biocoenosis is composed by euryhaline species, with numerous live individuals in waters with low hydrodynamics and important vegetation. The coastal and, to a lesser extent, the open sea association were introduced during the tidal flux; in addition, these assemblages may be collected in some secondary channels, owing to the erosion of fossil spits.El análisis de 69 muestras del Subsistema Carreras ha permitido diferenciar 10 distribuciones granulométricas (cinco de canal, cuatro de borde de canal y uno de marisma salada. El análisis geoquímico efectuado en algunas muestras revela un bajo grado de contaminación en los sedimentos superficiales. El estudio de la fauna permite diferenciar cuatro asociaciones de ostrácodos: a asociación estuarina sensu strictu (Loxoconcha elliptica, Cyprideis torosa, Cytherois fischeri, Elofmnia pusilla y Leptocythere sp., dominante en la biocenosis y tanatocenosis de todo el Subsistema; b asociaci

  8. Efecto de la exposición y la toposecuencia sobre actividades biológicas del suelo en bosque relicto del centro-sur de Chile Influence of slope and orientation on biological activities of a relict temperate forest from South-Central Chile

    Directory of Open Access Journals (Sweden)

    Francisco Reyes

    2011-01-01

    Full Text Available Las alteraciones catastróficas en ecosistemas boscosos son fenómenos naturales o antropogénicos que promueven la dinámica natural de la biocenosis y los procesos biogeoquímicos, y constituyen la principal causa de fragmentación del paisaje. El objetivo de este estudio fue evaluar el efecto de la exposición y la toposecuencia sobre algunas actividades biológicas del suelo de un bosque relicto de la comunidad Lapagerio-Aextoxiconetum, en dos estaciones del año. Se recolectaron muestras de suelo (0-15 cm, con características de Andisol por cada uno de los niveles toposecuenciales (laderas alta, media y baja con exposición noreste y suroeste, en verano e invierno. Los parámetros evaluados fueron: hidrólisis de la fluoresceína diacetato, carbono y nitrógeno en la biomasa microbiana y la actividad de seis enzimas relacionadas con la actividad microbiológica: carboximetilcelulasa, β-glucosidasa, manganeso peroxidasa, ureasa, fosfatasa ácida y arilsulfatasa. Los resultados obtenidos indican que la ladera alta con exposición noreste presentó condiciones más favorables para las actividades biológicas. La exposición tuvo efectos directos sobre las actividades biológicas, mayoritariamente significativos, debido a la variación del suelo en humedad, temperatura, pH, contenido de materia orgánica y disponibilidad de nutrientes. La ureasa fue la única enzima con mayor actividad en verano, debido al aumento de la temperatura del suelo. Las actividades biológicas fueron afectadas significativamente por la estacionalidad, siendo mayores en invierno, donde las diferencias observadas para los distintos parámetros biológicos se atribuyeron a condiciones de microclima que se generan bajo el dosel arbóreo y el mantillo.Catastrophic disturbances of forest ecosystems are natural and anthropogenic phenomena that promote the natural dynamics of biocenosis and biogeochemical processes, and are the main causes of landscape fragmentation. The

  9. Survey of the mosquitoes (Diptera: Culicidae of Mayotte.

    Directory of Open Access Journals (Sweden)

    Gilbert Le Goff

    Full Text Available A transversal survey of immature mosquitoes was conducted on Mayotte Island (France in the Comoros Archipelago, western Indian Ocean, with the aim to inventory the Culicidae and to document inter-species relationships in different habitats. In total 420 habitats were sampled for larvae and/or pupae mosquitoes, resulting in more than 6,000 specimens. Forty species belonging to 15 genera were collected, with eight taxa integrated for the first time to the Mayotte mosquito list. The most frequently recorded species were Stegomyia aegypti, St. albopicta, Anopheles gambiae and Eretmapodites subsimplicipes, the first three species being known vectors of viruses and parasites transmitted to humans. Mean species richness in habitats ranged from 1.00 to 3.29, with notable differences between habitats. For example, water-filled axils of banana leaves, tree-holes and crab-holes had low species richness, while cut bamboo, water pools, abandoned tires and marsh and swamp water had notably higher species richness. Twenty-seven mosquito species belonging to 12 genera were routinely collected (in ≥20% of at least one type of larval habitat suggesting that multiple species play a role in the biocenosis of these aquatic habitats. Multispecies association was observed in 52% of the habitats. The co-occurrence of up to six species belonging to five genera was recorded in a single habitat. The mosquitoes of Mayotte show notable biogeographical affinities to those of Madagascar, as compared to the African continent. These two potential source areas are nearly equidistant from Mayotte, which in turn indicates biased dispersal from east to west. Our findings suggest that with relatively short-term intensive sampling in different habitats, it is possible to approach exhaustive species inventories based on collection of larvae. Mayotte, with its modest elevation range and land surface, has a notable species richness of mosquitoes with 45 well-documented species

  10. Dynamics of toxicity within different compartments of a peri-urban river subject to combined sewer overflow discharges.

    Science.gov (United States)

    Becouze-Lareure, C; Thiebaud, L; Bazin, C; Namour, P; Breil, P; Perrodin, Y

    2016-01-01

    Combined Sewer Overflows (CSO) in small peri-urban streams and rivers are potentially toxic for their biocenosis. Improving the management of CSO discharges requires better knowledge of their dynamics and toxicity. In view to characterizing this toxicity, we sampled the different compartments (benthic and hyporheic zone) of a peri-urban stream located near the city of Lyon in France. The samples were taken at different distances from a CSO and at three period characteristic of different hydrological conditions. Their toxic effects were assessed by bioassays on the dissolved fraction (D. magna, V. fisheri and B. calyciflorus bioassays) and on the particle fraction (V. fisheri and H. incongruens bioassays). The results highlighted significant toxicity of the particulate fraction for the benthic and hyporheic samples, in particular downstream of the CSO, but with high spatio-temporal variability. This variability can first be attributed to the variability of CSO discharge sampling as a function of season and rainfall, and the dynamics of polluted particles (trapping of transported particles in infiltration zones, mobilization during floods). These parameters play a fundamental role in the distribution of pollutants according to the geomorphology of stream facies. Regarding dissolved pollutants, the chemical exchanges taking place at the "water-sediment" interface trigger the transfer of pollutants from one phase to another, after which the dispersion of these pollutants is governed by hydraulic flows. Finally, critical zones and periods are identified for the peri-urban river toxicity studied: benthic sediments under mean flow downstream; hyporheic sediments after a storm event downstream, close to the CSO. Recommendations are made on the basis of the knowledge obtained to optimize the management of these discharges. PMID:26379263

  11. Heavy metals in earthworms of Baden-Wuerttemberg. Part 1. Metal contents in earthworms from long-term forest observation plots; Schwermetalle in Regenwuermern Baden-Wuerttembergs. Teil 1. Metallgehalte in Regenwuermern von Wald-Dauerbeobachtungsflaechen

    Energy Technology Data Exchange (ETDEWEB)

    Rahtkens, K.; Trenck, K.T. von der [Landesanstalt fuer Umwelt, Messungen und Naturschutz Baden-Wuerttemberg (LUBW), Karlsruhe (Germany)

    2006-08-15

    Since 1984 the Environmental Protection Agency (LfU) of the State of Baden-Wuerttemberg has been investigating the heavy metal burden of earthworms from representative long-term forest observation plots. These investigations are aimed at elucidating and assessing adverse effects of pollutants on the soil biocenosis. Methods: At first only lead and cadmium were measured in the worms; in repetitive measurements over the years further metals or metalloid elements such as aluminum, arsenic, chromium, cobalt, copper, manganese, mercury, nickel, thallium, vanadium, and zinc were added to the agenda. Results and conclusion: The frequency distribution of the metals in the earthworms was characterized statistically. For the first time state-wide background values, normal values and threshold values were established for all the elements measured. A comparable study with a similar range of parameters is known neither nationally nor internationally. Time trend studies have been available for lead and cadmium since 1985/86, and for zinc since 1987. The studies show a steady decrease of the heavy metals on a state-wide average and especially in the 'Odenwald' and 'Black Forest' hills with their formerly highly polluted earthworm habitats. Today, the level of these metals in the earthworms is low with few exceptions. This holds true for other heavy metals as well. Recommendations and perspectives: The state-wide decreasing very low heavy metal burden of forest observation plots far from known emission sources can be considered a success of air pollution abatement measures. Because of the ongoing atmospheric deposition of heavy metals, regular observation should continue in order to estimate time trends and to recommend the reduction of emissions when precautionary values (part 2) are exceeded. (orig.)

  12. Characterization of Salmonella occurring at high prevalence in a population of the land iguana Conolophus subcristatus in Galapagos Islands, Ecuador.

    Directory of Open Access Journals (Sweden)

    Alessia Franco

    Full Text Available The aim of the study was to elucidate the association between the zoonotic pathogen Salmonella and a population of land iguana, Colonophus subcristatus, endemic to Galápagos Islands in Ecuador. We assessed the presence of Salmonella subspecies and serovars and estimated the prevalence of the pathogen in that population. Additionally, we investigated the genetic relatedness among isolates and serovars utilising pulsed field gel electrophoresis (PFGE on XbaI-digested DNA and determined the antimicrobial susceptibility to a panel of antimicrobials. The study was carried out by sampling cloacal swabs from animals (n = 63 in their natural environment on in the island of Santa Cruz. A high prevalence (62/63, 98.4% was observed with heterogeneity of Salmonella subspecies and serovars, all known to be associated with reptiles and with reptile-associated salomonellosis in humans. Serotyping revealed 14 different serovars among four Salmonella enterica subspecies: S. enterica subsp. enterica (n = 48, S. enterica subsp. salamae (n = 2, S. enterica subsp. diarizonae (n = 1, and S. enterica subsp. houtenae (n = 7. Four serovars were predominant: S. Poona (n = 18, S. Pomona (n = 10, S. Abaetetuba (n = 8, and S. Newport (n = 5. The S. Poona isolates revealed nine unique XbaI PFGE patterns, with 15 isolates showing a similarity of 70%. Nine S. Pomona isolates had a similarity of 84%. One main cluster with seven (88% indistinguishable isolates of S. Abaetetuba was observed. All the Salmonella isolates were pan-susceptible to antimicrobials representative of the most relevant therapeutic classes. The high prevalence and absence of clinical signs suggest a natural interaction of the different Salmonella serovars with the host species. The interaction may have been established before any possible exposure of the iguanas and the biocenosis to direct or indirect environmental factors influenced by the use of antimicrobials in

  13. Characterization of Salmonella occurring at high prevalence in a population of the land iguana Conolophus subcristatus in Galápagos Islands, Ecuador.

    Science.gov (United States)

    Franco, Alessia; Hendriksen, Rene S; Lorenzetti, Serena; Onorati, Roberta; Gentile, Gabriele; Dell'Omo, Giacomo; Aarestrup, Frank M; Battisti, Antonio

    2011-01-01

    The aim of the study was to elucidate the association between the zoonotic pathogen Salmonella and a population of land iguana, Colonophus subcristatus, endemic to Galápagos Islands in Ecuador. We assessed the presence of Salmonella subspecies and serovars and estimated the prevalence of the pathogen in that population. Additionally, we investigated the genetic relatedness among isolates and serovars utilising pulsed field gel electrophoresis (PFGE) on XbaI-digested DNA and determined the antimicrobial susceptibility to a panel of antimicrobials. The study was carried out by sampling cloacal swabs from animals (n = 63) in their natural environment on in the island of Santa Cruz. A high prevalence (62/63, 98.4%) was observed with heterogeneity of Salmonella subspecies and serovars, all known to be associated with reptiles and with reptile-associated salomonellosis in humans. Serotyping revealed 14 different serovars among four Salmonella enterica subspecies: S. enterica subsp. enterica (n = 48), S. enterica subsp. salamae (n = 2), S. enterica subsp. diarizonae (n = 1), and S. enterica subsp. houtenae (n = 7). Four serovars were predominant: S. Poona (n = 18), S. Pomona (n = 10), S. Abaetetuba (n = 8), and S. Newport (n = 5). The S. Poona isolates revealed nine unique XbaI PFGE patterns, with 15 isolates showing a similarity of 70%. Nine S. Pomona isolates had a similarity of 84%. One main cluster with seven (88%) indistinguishable isolates of S. Abaetetuba was observed. All the Salmonella isolates were pan-susceptible to antimicrobials representative of the most relevant therapeutic classes. The high prevalence and absence of clinical signs suggest a natural interaction of the different Salmonella serovars with the host species. The interaction may have been established before any possible exposure of the iguanas and the biocenosis to direct or indirect environmental factors influenced by the use of antimicrobials in agriculture

  14. Biochemical characterization of cholinesterases in Enchytraeus albidus and assessment of in vivo and in vitro effects of different soil properties, copper and phenmedipham.

    Science.gov (United States)

    Howcroft, C F; Gravato, C; Amorim, M J B; Novais, S C; Soares, A M V M; Guilhermino, L

    2011-01-01

    Enchytraeus albidus are important organisms of the soil biocenosis, used as standard test species in environmental risk assessment. The inhibition of cholinesterases (ChE) activity of several species has been widely used to assess the exposure and effects of anti-cholinesterase environmental contaminants. Several studies have shown the association between ChE activity inhibition and adverse effects on behaviour and survival. Extensive studies addressing survival and behavioural endpoints, as well as other biomarkers, have been done in E. albidus with different types of soil contaminants. The main objectives of this study were: (1) to characterize biochemically the ChE present in the soluble post-mitochondrial fraction of E. albidus whole body homogenates, using different substrates and selective inhibitors; (2) to assess the in vivo effects of copper, phenmedipham and different soil properties (pH, organic matter, clay) on the ChE activity; (3) to assess the in vitro effects of copper and phenmedipham on the ChE activity. The results suggest the presence of one ChE in the soluble post-mitochondrial fraction of E. albidus whole body homogenates, which displays properties of both acetylcholinesterase and pseudocholinesterase considering the typical mammalian enzymes. It is also shown that ChE activity is not inhibited by exposure to different soil properties and that copper and phenmedipham inhibited ChE activity both in in vivo and in in vitro conditions and therefore ChE inhibition seems to be a robust biomarker for this herbicide and this heavy metal. This study showed that ChE activity in E. albidus might be correlated to previously determined higher level effects like survival and reproduction, as well as avoidance behaviour. PMID:21080225

  15. Effects of thermal effluents from the Unterweser reactor (KKU) on biocenoses in the Unterweser. Pt. 3. Final report. Auswirkungen der Abwasserwaerme des Kernkraftwerkes Unterweser (KKU) auf die Biozoenosen in der Unterweser. T. 3. Abschlussbericht

    Energy Technology Data Exchange (ETDEWEB)

    Vobach, M.; Feldt, W.

    1991-05-01

    Between August 1975 and November 1982, the influence of thermal pollution in the biocenosis of the Unterweser from cooling water dischanged by the Unterweser reactor was investigated. The state of the parts of the river not yet stressed by cooling water is compared with conditions after the start-up of the reactor (September 1978). The seasonal cycle of water temperature has not changed after the start-up as compared to the time before. A warming of the river water because of cooling water discharged from the reactor is recognizable in the area immediately surrounding the month of the discharge system. Benthal investigations show the composition of species and number of individuals to be unchanged after the start-up of the reactor. Phytoplankton, too, continues to have its population maximum in May and August. Zooplankton, being present abundantly and in clusters, has retained its original composition of species. Now as before the reactor's start-up, flounder, smelt, stickleback, sprat and gudgeon which between them account for 97 per cent of the total catch, continue to be the five major fish species. Variations in the composition of catch are not to be explained by changes of temperature. The slight temperature increase does not modify the spectrum of species; there is no temperature stimulus. The seasonal cycle of water temperatures, which are important for a diapause, i.e. a slowing in the developmental cycle with a reduced metabolism, thus safeguarding the survival of certain species, do continue to occur. Any observed changes are to be interpreted as expressions of longer-term biological cycles. (orig./BBR).

  16. [Pre-and probiotics effectiveness in intestine microbiocenosis correction in patients after hemicolectomy].

    Science.gov (United States)

    Li, A I; Sil'vestrova, S Iu

    2011-01-01

    Normal microflora plays a key role in ensuring and maintaining the health of human body. Currently, much attention is paid not only to rational, but the so-called optimal, or health nutrition, which includes individual selection of food, the most satisfying human needs for energy, plastic and regulatory components. The aim of our study was to evaluate the effectiveness of pre-and probiotics in correction of the intestine microbiocenosis, effect on the rate of the hyperendotoxemia and related functional changes in the liver and lipid metabolism. In our study, we identified changesof the intestine microbiocenosis, which led to endotoxemia to develop, and the associated changes in liver enzymes and lipid blood composition in patients after various variants of the hemicolectomy. During treatment were selected 50 patients with dysbiosis 2 - 4-th degree who underwent hemicolectomy. In the 1st groupwere patients (25 pat.) with the prevailing complaints of constipation that were performed therapy with lactulose prebiotics (Dyufalak) at 20 - 30 ml per day for 3 months. In the 2nd group were patients (25 pat.) with complaints of diarrhea and was conducted therapy with bifiform probiotic in a daily dose to 2 capsules 2 times a day for 3 months. The results of studies performed after treatment showed that the admission of pro-and prebiotics in patients after hemicolectomy, has a high efficiency in micro biocenosis restoring and had a positive effect not only on bacterial composition of the microflora, but also on the functional state of the liver and lipid levels in patients serum. PMID:22168078

  17. Fermentation of organic waste - fundamentals and optimization potentials; Vergaerung von Bioabfaellen - Grundlagen und Optimierungspotentiale

    Energy Technology Data Exchange (ETDEWEB)

    Faulstich, M.; Christ, O. [Technische Univ. Muenchen, Garching (Germany). Inst. fuer Wasserguete- und Abfallwirtschaft

    1998-10-01

    For plants of a size of less than 2000 Mg per year, two-stage systems are not to be recommended as they are technically more demanding. It may suffice to install a simple dry-fermentation stage preceding an open composting stack in order to reduce odorous emissions. For large plants for urban catchment areas it is economical to have two-stage processes. Whether separation of hydrolysis and methanization saves substantial costs must be verified in each individual instance, as smaller volumes require higher technical effort and energy input. The separation of biocenoses represents an interesting fermentation concept as the advantages of each biocenosis complement each other and performance can be much enhanced. Optimum choice of a process is only possible considering local conditions. The authors have tried to give a systematized overview of fermentation processes to permit choosing the `appropriate` technique, independently of suppliers. (orig./SR) [Deutsch] Fuer Anlagen einer Groesse unter 2.000 Mg im Jahr, sind die technisch aufwendigeren zweistufigen Anlagen nicht zu empfehlen. Es kann daher ausreichend sein, einer offenen Mietenkompostierung ein einfaches Trockenfermentationsverfahren vorzuschalten, um die Geruchsemissionen zu reduzieren. Grosse Anlagen fuer innerstaedtische Einzugsgebiete koennen wirtschaftlich mit zweistufigen oder zweiphasigen Verfahrenstechniken ausgeruestet werden. Ob durch die Trennung von Hydrolyse und Methanisierung wesentliche Kosteneinsparungen zu erzielen sind, ist im Einzelfall zu pruefen, da geringfuegige kleinere Volumina durch entsprechende Regelungsstrategien erkauft werden. Die Trennung der Biozoenosen stellt ein interessantes Vergaerungskonzept dar, da sich die Vorteile beider Biozoenosen ergaenzen und damit wesentliche Leistungssteigerungen zu erzielen sind. Eine optimale Verfahrensauswahl ist nur unter Beruecksichtigung der oertlichen Verhaeltnisse sinnvoll. Es wurde daher versucht, die Moeglichkeiten zur Wahl eines

  18. [Study of vaginal and intestinal microflora of women in the prenatal period and its correction in dysbacteriosis].

    Science.gov (United States)

    Podgorskiĭ, V S; Liaskovskiĭ, T M; Kovalenko, N K; Oleshchenko, L T

    2006-01-01

    A comparative research of the structure of vaginal and intestinal microflora in norm and at kidney pathologies in women in the prenatal period was carried out. At physiologically normal course of pregnancy microbiocenosis of vagina and intestine does not contain pathogenic and conditional-pathogenic microorganisms. Lactic acid bacteria are fixed within the limits of 10(5)-10(8) CFU/1 ml in the vagina secretion and 10(7)-10(8) CFU/1 g in the intestine content. The spore microorganisms have completely disappeared in vaginal biocenosis of women with pyelonephritis after pharmacotherapy; and colibacterias has disappeared in 62% of women. Quantity of lactic acid bacteria has also decreased. Quantity of E. coli (10(8)- 10(9)) CFU/g, sporous microorganisms (10(3)-10(4)) CFU/g and yeast (10(4)-10(5)) CFU/g has considerably increased in the content of intestine. Lactic acid bacteria in content of the vagina are presented by: Lactobacillus acidophilus, L. plantarum, L. fermentum, L. vaginalis; in content of the intestine--L. plantarum and L. fermentum. All the isolated strains of lactobacilli are resistant to metrogyl and amphotericin, 87%--to cephoxitine, cypropfloxacine. Twenty three of 24 strains of lactic acid bacteria--antagonists of pathogenic microflora have shown adhesion to vaginal epthelium. The degree of adhesive activity depends on the strain and genus specificity of cultures. The highest index of adhesiveness in indicated in Enterococcus faecium (5.82 - 7.62) and Streptococcus salivarius subsp. thermophilus (2.74 - 6.40) strains. The screening of strains has been performed, which suppress growth of gas-producing microflora. The strain of Lactobacillus plantarum 200D proved to be the most active one. A complex of cultures of the lactic bacteria providing normalization of microbial cenosis of the vagina in women in the prenatal period has been selected as based on the data obtained. The biotechnology of production of vaginal suppositories is developed. PMID

  19. Survey of the mosquitoes (Diptera: Culicidae) of Mayotte.

    Science.gov (United States)

    Le Goff, Gilbert; Goodman, Steven M; Elguero, Eric; Robert, Vincent

    2014-01-01

    A transversal survey of immature mosquitoes was conducted on Mayotte Island (France) in the Comoros Archipelago, western Indian Ocean, with the aim to inventory the Culicidae and to document inter-species relationships in different habitats. In total 420 habitats were sampled for larvae and/or pupae mosquitoes, resulting in more than 6,000 specimens. Forty species belonging to 15 genera were collected, with eight taxa integrated for the first time to the Mayotte mosquito list. The most frequently recorded species were Stegomyia aegypti, St. albopicta, Anopheles gambiae and Eretmapodites subsimplicipes, the first three species being known vectors of viruses and parasites transmitted to humans. Mean species richness in habitats ranged from 1.00 to 3.29, with notable differences between habitats. For example, water-filled axils of banana leaves, tree-holes and crab-holes had low species richness, while cut bamboo, water pools, abandoned tires and marsh and swamp water had notably higher species richness. Twenty-seven mosquito species belonging to 12 genera were routinely collected (in ≥20% of at least one type of larval habitat) suggesting that multiple species play a role in the biocenosis of these aquatic habitats. Multispecies association was observed in 52% of the habitats. The co-occurrence of up to six species belonging to five genera was recorded in a single habitat. The mosquitoes of Mayotte show notable biogeographical affinities to those of Madagascar, as compared to the African continent. These two potential source areas are nearly equidistant from Mayotte, which in turn indicates biased dispersal from east to west. Our findings suggest that with relatively short-term intensive sampling in different habitats, it is possible to approach exhaustive species inventories based on collection of larvae. Mayotte, with its modest elevation range and land surface, has a notable species richness of mosquitoes with 45 well-documented species belonging to 15 genera

  20. Trophic chain and sea environment self-cleaning factors

    Directory of Open Access Journals (Sweden)

    Pogorel'tsev Yuriy Romanovich

    2014-05-01

    Full Text Available This article considers the main aspects of the process of self-purification in the marine environment. It describes mechanics of biogenic elements of the marine environment in the process of production and destruction of autochthonous and allochthonous organic matter. This article discusses organics and flows of energy, which migrate to the trophic chain of the marine environment in the process of self-purification. And it shows the individual elements of the process of self-purification in the marine environment and the factors influencing it. In the article it is noted that self-cleaning of water environment happens due to the cycling of matter in the pond. It is emphasized that tension, focus and self-purification completeness are regulated by biotic turnover and energy turnover, which is determined by the type of limnological type of reservoir, geographical features (climate conditions, geophysical and anthropogenic impacts. The article notes that the more diverse system of organisms is, the fuller the compounds’ decay is. This property of organisms to complement each other is called buffering of the system. Complex system of organisms cope better with organic and bacterial contamination, but is less responsive to insertion nutrients; biotic cycle in complex systems is more intense. Bacterial community plays the major role in the process of self-purification of biological marine environments. They are the major element of the coastal zone ecosystems. This article shows that during the growth of bacterial populations most of the energy supplied to the aquatic ecosystems with auto- and allochthonous organic matter is processed. The bacteria prepare the conditions for the development of other organisms of water biocenosis. Concentration of the organic substrate regulates the growth rate of bacteria. Bacterial self-cleaning depends on the total number of microorganisms or their separate groups, locally contained in the marine environment.

  1. Winter and spring mixing depths affect the trophic status and composition of phytoplankton in the northern meromictic basin of Lake Lugano

    Directory of Open Access Journals (Sweden)

    Marco SIMONA

    2003-08-01

    Full Text Available The trophic state of Lake Lugano is still too high to be acceptable, despite extensive recovery measures undertaken in recent decades which have resulted in a reduction of the external phosphorus load to the deepest of the lake's basins (northern basin; Zmax=286 m to fairly acceptable values. Since meromixis was established in the middle of last century, the deep hypolimnion of the northern basin (the layer between ca 100 m and the bottom has contained high quantities of nutrients (especially phosphorus which are a major potential source of internal load. When there are particularly strong winter mixing events, a portion of this phosphorus reserve is redistributed along the upper water column (0-100 m. The impact of meteo-climatic conditions on the plankton biocenosis were analysed using data collected in the northern basin (Gandria station during the three-year period 1998-2000. The phytoplankton composition, which is typical of eutrophicated waters, shows marked interannual variations, also depending on the degree of mixing of the waters at the start of the vegetative period. Though there is no steady pattern of typical dominant species / master species in the lake, there is a seasonal succession characterised by a marked development of diatoms in spring, and a predominance of chlorophyceans and cyanobacteria in summer and autumn. Under present conditions, the mechanisms of internal replenishment of nutrients towards the euphotic layer, due to the phenomena of late winter and spring mixing, constitute a significant source of nutrients for the spring and summer growth of phytoplankton. On the other hand, pronounced mixing phenomena, like those occurring in the two-year period 1999-2000, can reduce the hypolimnetic nutrient reserves and cause a decrease in the trophic potential of the basin, contrasting with an increase in algal biomass in the euphotic zone.

  2. 生态工程自然化恢复米勒河滨水生态%Riverine Ecology Restoration through Ecological Engineering and Naturalization

    Institute of Scientific and Technical Information of China (English)

    胡先祥; 汪洋; 陈林; 阮维桢; 尹茜

    2013-01-01

    [目的]为改善和恢复埃塞俄比亚加里示范区米勒河滨水及河流遭到破坏的生态系统,防止生物多样性严重下降或丧失.[方法]基于演替理论,通过生态工程和”自然化”结合的方法,保护自然和恢复湿地.[结果]通过重建湿地的生物群落,恢复河流生态环境,生态功能明显恢复.[结论]多体系配套生态工程在东非半干旱地区是可行的,其恢复实践为保护和恢复当地水生态资源做出了建设性示范.%The aim was to improve and restore destroyed riverine and water ecosystem and to prevent bio-diversity from deteriorating in Jari demonstration plot in Ethiopia.[Method] Biological engineering and naturalization were made use of to protect nature and restore wetland as per Succession Theory.[Result] Both of ecoenvironment and eco-functions of Mille River were restored through reconstruction of biocenosis in wetlands.[Conclusion] It is feasible to implement matched ecological projects in semiarid regions in East Africa,providing references for restoration and protection of local water resources.

  3. An integrated approach to manage coastal ecosystems and prevent marine pollution effects

    Science.gov (United States)

    Marcelli, Marco; Bonamano, Simone; Carli, Filippo Maria; Giovacchini, Monica; Madonia, Alice; Mancini, Emanuele; Molino, Chiara; Piermattei, Viviana; Manfredi Frattarelli, Francesco

    2016-04-01

    benthic biocenosis as relevant spatial unit, and the ESI calculation for Civitavecchia coastal areas, are reported.

  4. Gamma background irradiation. Standards and reality

    International Nuclear Information System (INIS)

    The systematic deviation of the results of measuring the power of air dose absorbed from the natural gamma background radiation in Bulgaria is inadmissibly large and variable. This in turn augments the dispersion of results as well as the mean value relative to worldwide data, to an implausible level, hardly attributable to the variegated geographical relief of the country. Thus in practice local anthropogenic increases hardly lend themselves to detection and demonstration. In the Radiation Protection Standards (RPS-92) in effect in Bulgaria, and in other documents concerning the same radiation factors as well, the maximum allowable limits for the population as a whole are clearly specified on the basis of worldwide expertise along this line. As a rule these limits are being exceeded by the actually measured values, and for this reason the cited documents contain a clause stipulating that these limits do not refer to the natural radiation background and therefore the latter may be virtually ignored. Thus the basic risk factor for the population goes beyond control at levels commensurable with the officially established limits, its twofold increase inclusive. The maximum allowable limit becomes undefinable. Bearing in mind the fact that in compliance with the cited RPS-92 elimination of the technogenic ionizing radiation sources incorporated in the environment prior to 1992 is 'freezed', it is evident that exposure of the population to anthropogenic radiation becomes legally allowable in a much wider range than the one specified by world legislators. One may anticipate radiation induced health noxae for the population directly or by anthropogenic radiation stress on biocenosis. A relatively large part of the population is susceptible to the effect of low radiation doses. Presumably this contingent will augment as a result of eventual fluctuations. The casual relationship which is difficult to establish should be given due consideration in the analysis of the causes

  5. Microbial composition in a deep saline aquifer in the North German Basin -microbiologically induced corrosion and mineral precipitation affecting geothermal plant operation and the effects of plant downtime

    Science.gov (United States)

    Lerm, Stephanie; Westphal, Anke; Miethling-Graff, Rona; Alawi, Mashal; Seibt, Andrea; Wolfgramm, Markus; Würdemann, Hilke

    2013-04-01

    The microbial composition in fluids of a deep saline geothermal used aquifer in the North German Basin was characterized over a period of five years. The genetic fingerprinting techniques PCR-SSCP and PCR-DGGE revealed distinct microbial communities in fluids produced from the cold and warm side of the aquifer. Direct cell counting and quantification of 16S rRNA genes and dissimilatory sulfite reductase (dsrA) genes by real-time PCR proved different population sizes in fluids, showing higher abundance of Bacteria and sulfate reducing bacteria (SRB) in cold fluids compared to warm fluids. Predominating SRB in the cold well probably accounted for corrosion damage to the submersible well pump, and iron sulfide precipitates in the near wellbore area and topside facility filters. This corresponded to a lower sulfate content in fluids produced from the cold well as well as higher content of hydrogen gas that was probably released from corrosion, and maybe favoured growth of hydrogenotrophic SRB. Plant downtime significantly influenced the microbial biocenosis in fluids. Samples taken after plant restart gave indications about the processes occurring downhole during those phases. High DNA concentrations in fluids at the beginning of the restart process with a decreasing trend over time indicated a higher abundance of microbes during plant downtime compared to regular plant operation. It is likely that a gradual drop in temperature as well as stagnant conditions favoured the growth of microbes and maturation of biofilms at the casing and in pores of the reservoir rock in the near wellbore area. Furthermore, it became obvious that the microorganisms were more associated to particles then free-living. This study reflects the high influence of microbial populations for geothermal plant operation, because microbiologically induced precipitative and corrosive processes adversely affect plant reliability. Those processes may favourably occur during plant downtime due to enhanced

  6. Extended abstracts for an international conference on the development of the North and problems of recultivation

    Energy Technology Data Exchange (ETDEWEB)

    Everett, K.R. [ed.

    1994-12-31

    Ecological problems in many regions on Earth are the result of increasing technological pressure on the environment. These problems concern many of us and cause mankind to unite in order to search for means to protect the environment. Scientists, especially are responsible for the protection of the biosphere. The objective of this conference was to discuss the results of studies on the present condition of the environment in the Far North where the industrial pressure is increasing. The participants of this conference also offered and suggested various necessary measures for the protection of the region and restoration of its disturbed sites. The specific structural characteristics of the environment of the Far North, tundra and northern taiga, cause its fragility and vulnerability to anthropogenic impact. The destruction of the thin, weak layer of soil and vegetation cover changes the thermal balance and thus causes the development of erosion process, which in their turn increase the zone of the direct technogenous destruction. Self restoration processes in this harsh climate usually are slow. The preservation of the ecological integrity in the Far North is essential for the stability of the biosphere of the planet. The specifics of the natural conditions must be taken into account so that man will be able to develop the means of intensive agro-technology that can speed up the process of restoration of the biocenosis in the damaged areas. The extended abstracts of the conference reports that constitute this volume contain both theoretical discussions of problems of recultivation as well as accounts of experimental studies and applied explorations.

  7. The use of biotopes in assessing the environmental quality of tidal estuaries in Europe

    Science.gov (United States)

    Ducrotoy, Jean-Paul

    2010-02-01

    In Europe, the Water Framework Directive (WFD) (European Commission 2000) - and the recently proposed EU Marine Strategy Directive - have established a framework for the protection of groundwater, inland surface waters, estuarine (transitional) waters and coastal waters. TheWFD has several objectives: to prevent water ecosystem deterioration, to protect and to enhance the status of water resources but the most important aspect is to achieve a 'Good Ecological Status' (GES) for all waters, by 2015. In essence, the WFD requires a water body to be compared against a reference condition and then its ecological status designated - if the water body does not meet good or high ecological status, i.e. it is in moderate, poor or bad ecological status, then remedial measures have to be taken (e.g. pollution has to be removed). Many indices were developed from benthic work and are often thought fit for purpose. Based on the successional model proposed by Pearson and Rosenberg (1978), most of these indices were effectively established for soft sediment benthos. However, those developed in the framework of the WFD were derived from work on the subtidal. They are difficult to use in the intertidal and in transitional waters. As they were derived from work on organic pollution, there is no or little evident link with chemical and physical pollution. Ecomorphology brings together a biological approach and a sedimentological approach to estuarine ecology. It considers the use of the biotope and related concepts (biocenosis, bio-facies, ecotone, habitat…) as a basis to a novel approach to environmental quality assessment. It addresses the problem of the estuarine quality paradox in recognising the role of nutrients and organic matter in biogeochemical cycles. The discussion shows the complementarity of biotopes with the Sato-Umi and the ecohydrolgy approaches.

  8. Effects of thermal effluents from the Unterweser reactor (KKU) on biocenoses in the Unterweser. Pt. 3

    International Nuclear Information System (INIS)

    Between August 1975 and November 1982, the influence of thermal pollution in the biocenosis of the Unterweser from cooling water dischanged by the Unterweser reactor was investigated. The state of the parts of the river not yet stressed by cooling water is compared with conditions after the start-up of the reactor (September 1978). The seasonal cycle of water temperature has not changed after the start-up as compared to the time before. A warming of the river water because of cooling water discharged from the reactor is recognizable in the area immediately surrounding the month of the discharge system. Benthal investigations show the composition of species and number of individuals to be unchanged after the start-up of the reactor. Phytoplankton, too, continues to have its population maximum in May and August. Zooplankton, being present abundantly and in clusters, has retained its original composition of species. Now as before the reactor's start-up, flounder, smelt, stickleback, sprat and gudgeon which between them account for 97 per cent of the total catch, continue to be the five major fish species. Variations in the composition of catch are not to be explained by changes of temperature. The slight temperature increase does not modify the spectrum of species; there is no temperature stimulus. The seasonal cycle of water temperatures, which are important for a diapause, i.e. a slowing in the developmental cycle with a reduced metabolism, thus safeguarding the survival of certain species, do continue to occur. Any observed changes are to be interpreted as expressions of longer-term biological cycles. (orig./BBR)

  9. Influence of shutdown phases on the microbial community composition and their effects on the operational reliability in a geothermal plant in the North German Basin

    Science.gov (United States)

    Westphal, Anke; Lerm, Stephanie; Miethling-Graff, Rona; Seibt, Andrea; Wolfgramm, Markus; Würdemann, Hilke

    2014-05-01

    Microbial activity can influence the dissolution and/or precipitation of minerals, as well as corrosion phenomena that may lead to a lower efficiency of engineered systems. To enhance the understanding of these processes, the microbial biocenosis in fluids produced from the cold well of a deep geothermal heat store located in the North German Basin (NGB) was characterized during normal plant operation and immediately after plant downtime phases. The microbial community composition was dominated by three different genera of sulphate reducing bacteria (SRB) and fermentative Halanaerobiaceae in the 46 ° C tempered fluids during regular operation, whereas after shut down phases sequences of sulphur oxidizing bacteria (SOB) were additionally detected. The detection of SOB is regarded as an indication of oxygen introduction into the well during the downtime phase. This corresponded to the higher redox potential of fluids taken directly after the restart of fluid production in the cold well. In addition to an extremely high particle loading rate after plant restart, a higher DNA content as well as an increase of specific gene copy numbers of SRB and SOB by a factor of 104 and 105 respectively were observed. Obviously stagnant conditions favored the enrichment of biomass and particles in the well. This is supported by the determination of a higher sulphate and hydrogen sulphide content in the fluids taken initially after plant restart. With increasing fluid production during the restart, SRB specific gene copy numbers decreased much slower than SOB specific gene copy numbers, which led to the assumption that SOB abundance is limited to the near wellbore area. Besides the absence of particle removal by fluid flow and the deposition of particles by sedimentation during the shut down phase, oxygen introduction and subsequent activity of SOB may also have favored microbial induced formation of precipitates in the well. It is quite likely that the interaction of SRB and SOB

  10. Aspects on the removal of hazardous substances from flue gas by the applicance of waste water treatment techniques. Final report. Abwassertechnische Aspekte bei der Schadstoffreduzierung aus Abgasen. Schlussbericht

    Energy Technology Data Exchange (ETDEWEB)

    Dreschmann, P.; Sterk, T.

    1988-01-01

    The objective of this investigation was the qualitative and quantitative assessment of condensates, the examination of changes to the characteristics of the waste water due to immission of condensates, the assessment of effects on communal waste water purification plants, the investigation of the possibilities of waste gas purification using communal waste water. The results of investigations have shown that discharge of untreated condensates will not be possible, with respect to local regulations, as the pH value of the condensates vary between 3.0 and 4.0 in the case of gas-firing, and between 1.7 and 2.5 in the case of oil-firing. As far as the other approximate values given by the ATV in the worksheet A 115 'Advice on discharging waste water into public sewage works' are concerned, for anions, cations and hydrocarbons, it was possible to demonstrate that such values were not exceeded in the case of gas condensates, whereas in the gas of oil condensates, depending on the furnace installation, values were at times exceeded. The discharge experiments, conducted with various mixtures of condensate/waste water, have shown that the neutralisation of condensates using communal waste water, depending on the furnace installation and the fuel used, is definitely capable of being implemented as a pretreatment technique. In order to prevent damage to the biocenosis and to guarantee nitrification in the waste water purification plant, an acidic capacity in the flow of k/sub S/ /sub 43/ /ge/ 6.5 mmol/l is required. Trials in waste gas purification using communal waste water of the City of Heidenheim produced elimination rates, in the case of oil-firing, for SO/sub 2/ of eta SO/sub 2/ = 88.1% with an inlet facility in the channel floor, and of eta SO/sub 2/ = 97.4% with a countercurrent cascade. In the case of gas-firing an NO/sub x/ elimination rate of eta NO/sub x/ = 12.8% was achieved. 133 refs., 48 tabs., 45 figs.

  11. Heavy metals in earthworms of Baden-Wuerttemberg. Pt. 2. Ecotoxicological assessment of soil; Schwermetalle in Regenwuermern Baden-Wuerttembergs. T. 2. Oekotoxikologische Bewertung des Bodens

    Energy Technology Data Exchange (ETDEWEB)

    Rahtkens, K.; Trenck, K.T. von der [Landesanstalt fuer Umwelt, Messungen und Naturschutz Baden-Wuerttemberg (LUBW), Karlsruhe (Germany)

    2007-02-15

    Goal and Scope. The heavy metal burden of the soil and of earth-worms from representative long-term forest observation plots has been measured since 1984 as one component of the media-embracing environmental monitoring network of the State of Baden-Wuerttemberg. These investigations are aimed at elucidating and assessing adverse effects of pollutants on the soil biocenosis. Methods. So-called characteristic curves for the metals Cd, Cu, Ni, Pb, and Zn were developed for the assessment. Earthworm toxicity data and background values in soil served as criteria. This procedure facilitates a comparative assessment of different pollutants. The mobility of the metals, which greatly influences their bioavailability and toxicity, was taken into account for the effects assessment. Results and Conclusion. Besides inventorying the heavy metals (Part 1), the question of threshold values for toxic reactions as well as for accumulation was raised. The metal concentrations were assessed in the soil with regard to its habitat function. The ecotoxicological assessment revealed that the heavy metal burden of the investigated plots lies within the background- or precautionary range, well below the screening value established here to indicate the effect threshold in earthworms. This result means that a pollution of the forest observation plots with the metals cadmium, copper, lead, nickel, and zinc is low or absent. Only the distribution of the lead content is centered slightly above background (but clearly below the toxicity threshold). This slight lead burden of the soil of the observation plots can be explained by emissions from motor vehicles. Investigations at sites that are contaminated with either chromium or copper or cadmium showed that an accumulation of these metals in the body of the worms can be observed only above a threshold concentration of several hundred micrograms of mobile metal per kilogram in the upper layer of the soil. Recommendations and Perspectives. The

  12. Carcass Composition of Turopolje Pig, the Autochthonous Croatian Breed

    Directory of Open Access Journals (Sweden)

    Marija Đikić

    2003-12-01

    Full Text Available Objective of this research was to establish carcass composition in Turopolje pig breed by analyzing the percentage of muscle (M, fat (F and bone (B tissue, percentage of these tissues in different parts of carcass (ham, loin, back, neck, belly-rib part . BRP, as well as share of the parts in carcass. Animals were divided into two groups of different age and slaughtering weight (TI 584 ± 20 days and 81,9 kg; TII 679 ± 20 days and 100,3 kg ± 4,9 kg. Purpose of this investigation was to define the standards for traits mentioned for the remainder of the population of Turopolje pig and to set the selection and production programs, as this breed has been under protection since 1993 and re-establishment since 1996 as cultural and biological heritage of Croatia and the World. In order to define Turopolje pig in production sense, we presented characteristics of the old breeds Mongolitza and Black Slavonian and some selected breeds. Pigs were fattened in the outdoor system of flood forests and marsh meadows biocenosis (Quercus robur . Deschampsietum caespitosae, according to traditional Croatian technology of low input (Ðikić et al., 2002.Slaughtering weight and weight of warm and cold carcass were measured on the slaughtering line. The halves were separately weight and dissected (tissues and parts according to Weninger et al., 1963 and by total dissection. Data were processed by statistical-mathematical procedure GSM and SAS software package (1996 and results were compared within and between the groups ( t-test.In groups TI and TII the percentages tissues in carcass were established to be as follows: M 38.2% and 40.5%, B 10.6% and 9.7%, respectively, and were significantly different ( P< 0.05, while share of F, 34.2% and 33.8% were not significantly different.Fattened pigs in group TI, in relation to TII, had lower relative share of muscle tissue in the carcass of loin, back, neck and BRP and higher share of fat tissue of neck and BRP, as well as

  13. Effects of natural and chemical stressors on Enchytraeus albidus: can oxidative stress parameters be used as fast screening tools for the assessment of different stress impacts in soils?

    Science.gov (United States)

    Howcroft, C F; Amorim, M J B; Gravato, C; Guilhermino, L; Soares, A M V M

    2009-02-01

    Enchytraeids are important organisms of the soil biocenosis. They improve the soil pore structure and the degradation of organic matter. These organisms are used in standardized testing, using survival and reproduction (6 weeks) as endpoints. The use of biomarkers, linked to ecologically relevant alterations at higher levels of biological organization, is a promising tool for Environmental Risk Assessment. Here, enchytraeids were exposed for different time periods (two days and three weeks) to different soils (OECD artificial soil, different compositions in its organic matter, clay or pH value, and LUFA 2.2 natural soil) and different chemicals (Phenmedipham and copper). The main question addressed in the present study was if the effects of chemicals and different soil properties are preceded by alterations at the sub-cellular level, and if these endpoints may be used reliantly as faster screening tools for the assessment of different stress conditions in soils. The parameters measured in E. albidus whole body were: lipid peroxidation (LPO), total glutathione (TG), as well as the enzymatic activities of superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR), glutathione peroxidase (GPx) and glutathione S-transferase (GST). The results showed that biomarker responses in E. albidus were significantly affected by the soil type (GST, CAT, GPx, GR and LPO) and the duration of exposure in OECD artificial soil (GST, GPx, GR, CAT and LPO) but not in LUFA 2.2 natural soil. For the abiotic factors studied, after 2 days, low pH decreased significantly the TG levels and the activities of CAT and GR,and low OM also significantly decreased CAT and GR activities. After 3 weeks, differences in soil properties caused a decrease in GR and GPx activities, whereas increased GST activity was observed due to low organic matter and pH. Copper significantly increased the activities of CAT, GPx and GR, and decreased the activity of GST after 2 days as well as inscreasing

  14. Comparative analysis of morphometric characteristics of bivalves Anodonta piscinalis from the reservoirs of techa cascade of Mayak Production Association

    Energy Technology Data Exchange (ETDEWEB)

    Yegoreichenkov, E.; Pryakhin, E.; Akleyev, A. [Urals Research Center for Radiation Medicine - URCRM (Russian Federation)

    2014-07-01

    The reservoirs R-11, R-10 and R-4 of the Techa cascade (Chelyabinsk region) are used as low-active radioactive wastes storages, and are separated from open hydrographical network by a system of dams and by-pass channels. The values of specific activity of radionuclides in water and bottom sediments increases in the row: R-11, R-10, R-4, and at the same time the dose rate for zoo-benthos is increased on an order from reservoir to reservoir. Bivalves, which are obligate filter feeders and produce a significant part of water community biomass, are an important part of energy and matter transformation chain inside the hydro-biocenosis. Among this group of animals, the Anodonta piscinalis, a massive bivalve, takes the most part in biomass of Techa reservoirs cascade. Several studies show that in polluted environment the shell morphology changes are possible: the ratio of most morphometric values changes; the level of fluctuated asymmetry and shell polymorphism increases. For morphological studies of bivalves populations, 34 specimens of Anodonta were taken from R-11, 43 specimens from R-10 and 4 specimens from R-4. The specimens selection was arranged in May 2013, using hydro-biological drag. The shell shape was analyzed using TPS DIG software. Besides the measurements of maximal shell length, shell height, and the distance between shell's top and maximally distant point of front edge of the shell were measured. The ratio between the length and the height of the shell, between the length and the distance from the top to the front edge were calculated. The data analysis was arranged using R statistics. As a result of the data analysis the significant difference between shell's length to height ratios were registered between populations of R-4 and R-10 (p = 0.002). The ratio of maximal length of the shell to the distance from the top to the front edge also significantly differs between the animals of the R-4 and R-10 reservoirs (?= 0.03). Between the animals of

  15. Lactobacillus for Vaginal Microflora Correction

    Directory of Open Access Journals (Sweden)

    Saule Saduakhasova

    2014-12-01

    Full Text Available Introduction. Despite the significant progress made in prevention, diagnosis, and treatment, there is still a high rate of vaginal dysbiosis in Kazakh women. The use of antibiotics in the treatment of vaginal dysbiosis contributes to the elimination of pathogens as well as microflora, which can lead to a decrease in local immunity and more favorable conditions for infection spread. The most physiologically safe and promising method for the restoration of vaginal biocenosis is the use of probiotics administered by a vaginal route.Methods. We have allocated 64 of cultures of Lactobacillus from the vaginal epithelium of healthy women of reproductive age and women with diagnosed bacterial vaginosis (BV. Identification of cultures was performed by PCR analysis of 16S ribosomal RNA.  Evaluation of biological significance was determined by the following criteria: high antagonistic activity against Candida albicans, Escherichia coli, Serratia marcescens, Proteus mirabilis, Klebsiella ozaenae, and Staphylococcus aureus; and production of hydrogen peroxide, resistance to antibiotics, adhesive activity. We studied the symbiotic relationship of selected biologically active of cultures to each other and received options for consortiums with  properties of  probiotics through co-cultivation.Results. Results of genotyping  showed that the isolated lactobacilli belong to the seven species: L. fermentum, L. salivarius, L. gasseri, L. crispatus, L. jensenii, L. plantarum, and L. delbrueskii. L. fermentum, L. salivarius, L. gasseri, and L. jensenii occur in women with suspected BV. The highest percentage of occurrence in the vagina of healthy women was L. fermentum (28%. Most strains of lactobacilli possess high inhibitory activity for all test-strains, except Candida albicans (37.5%. 56% of studied cultures revealed high adhesion to human erythrocytes. All lactobacillus strains were resistant to metronidazole, 80% to kanamycin, 57%  to vancomycin, and

  16. Vegetation Dynamics Depending on Ecological Particularities of Bozanta Mare (Maramures County-Romania Tailing Pound. Case Study

    Directory of Open Access Journals (Sweden)

    Monica Marian

    2009-01-01

    Full Text Available The present study follows an ecological demarch of reintegration in the scenary through revegetation of an anthropic ground, consisting in a waste pond formed from the flotation activity of non-ferrous ores. Problem statement: To support the formation of a compact vegetal layer, having an anti-errosion and a restoration role, a preliminary study was required regarding the spontaneous settlement of different vegetal species. We have followed the specific floristic composition and the biodiversity on the waste pond, the manner of association of plant species, and the possible interractions with other species from the biocenosis (microorganisms and fungi. We have also studied the pace at which vegetal species settle, as well as the reciprocal influence, from the point of view of vegetation, with the neighbouring area, since the desideratum is the settlement of a vegetation similar to the natural one. Approach: The aim of the research is to draw a list of the vegetal taxa installed on the pond, as well as to detect some succession stages or some possible vegetal associations. We have established the share of different species in the vegetal layer on the waste pond through an analysis of the ecological preferences, of the geographical origin of plant species, of the cariological and bioform profile. All this was done to compare the possible vegetal associations which settle on such anthropic grounds with the neighbouring vegetation. The approach used was the classical one in fitosociology, recommended by the Central European Fitosociological School adapted to the pedo-climatic conditions in Romania. Results: Over 50 species of plants and fungi spontaniously settled have been listed, and we have followed their association as well as their distribution, compared to the microclimatical conditions of the waste pond. In this way,we have distinguished species with a large potential of revegetating highly polluted with heavy metals waste ponds and sites

  17. First sign of Clethrionomys glareolus in the Po valley plain / Prima segnalazione in Pianura Padana di Clethrionomys glareolus

    Directory of Open Access Journals (Sweden)

    Luis Nieder

    1987-07-01

    Full Text Available Abstract The presence of Clethrionomys glareolus (Schreber, 1790 populations is reported in the Po valley, formerly considered outside the distribution area of the species. An initial series of captures was made in the high-water area of the Po near S. Nazzaro (Monticelli d'Ongina - PC following with research was extended along the river's banks in Emilia both upstream and downstream. The search for this species was carried out by analysing food pellets relating to Strix aluco, Tyto alba e Athene noctua, thought to be among the most common predators of these intensive herbaceous and arboreal agricultural biocoenosis. The information we gathered was integrated with other Authors' unpublished and bibliographical data. The presence of C. glareolus was noted in habitats quite different from the expected ones. Many of the problems raised by the presence of this species in "islands" along the main body of the Po river and its tributaries, and by its presumed absence from particulary suitable environments such as the Carrega woods, the Ticino park and Mesola wood must still be resolved. Riassunto Viene segnalata la presenza di popolazioni di Clethrionomys glareolus (Schreber 1790 nella pianura Padana precedentemente considerata al di fuori dell'areale della specie. A partire da una prima serie di catture nell'area golenale del Po presso S. Nazzaro (Monticelli d'Ongina - PC, si è successivamente proceduto ad allargare l'area di ricerca lungo l'argine emiliano del Po, sia a monte sia a valle. La ricerca di questa specie è avvenuta tramite l'analisi di boli alimentari relativi a Strix aluco, Tyto alba e Athene noctua, ritenuti tra i più comuni predatori di queste biocenosi agricole intensive erbacee ed arboree. I dati da noi raccolti sono stati integrati con quelli bibliografici e inediti di altri Autori. È stata riscontrata la presenza

  18. Synthesis of Biomass and Utilization of Plant Wastes in a Physical Model of a Biological Life Support System

    Science.gov (United States)

    Tikhomirov, A. A.; Ushakova, S. A.; Manukovsky, N. S.; Lisovsky, G. M.; Kudenko, Yu A.; Kovalev, V. S.; Gribovksaya, I. V.; Tirranen, L. S.; Zolotukkhin, I. G.; Gros, J. B.; Lasseur, Ch.

    Biological life support systems (LSS) with highly closed intrasystem mass ex change mass ex change hold much promise for long-term human life support at planetary stations (Moon, Mars, etc.). The paper considers problems of biosynthesis of higher plants' biomass and "biological incineration" of plant wastes in a working physical model of biological LSS. The plant wastes are "biologically incinerated" in a special heterotroph block involving Californian worms, mushrooms and straw. The block processes plant wastes (straw, haulms) to produce soil-like substrate (SLS) on which plants (wheat, radish) are grown. Gas ex change in such a system consists of respiratory gas ex change of SLS and photosynthesis and respiration of plants. Specifics of gas ex change dynamics of high plants -SLS complex has been considered. Relationship between such a gas ex change and photosynthetic active radiation (PAR) and age of plants has been established. SLS fertility has been shown to depend on its thickness and phase of maturity. The biogenic elements (potassium, phosphorus, nitrogen) in Liebig minimum have been found to include nitrogen which is the first to impair plants' growth in disruption of the process conditions. The SLS microflora has been found to have different kinds of ammonifying and denitrifying bacteria which is indicative of intensive transformation of nitrogen-containing compounds. The number of physiological groups of microorganisms in SLS was, on the whole, steady. As a result, organic substances -products of ex change of plants and microorganisms were not accumulated in the medium, but mineralized and assimilated by the biocenosis. Experiments showed that the developed model of a man-made ecosystem realized complete utilization of plant wastes and involved them into the intrasystem turnover. In multiple recycle of the mat ter (more than 5 cycles) under the irradiance intensity of 150 W/m2 PAR and the SLS mass (dry weight) of 17.7 -19.9 kg/m2 average total harvest of

  19. Autochthonous microbe-assisted phytoremediation of brown coal mine overburden soil

    Science.gov (United States)

    Hamidović, Saud; Teodorović, Smilja; Lalević, Blažo; Karličić, Vera; Jovanović, Ljubinko; Kiković, Dragan; Raičević, Vera

    2015-04-01

    One of the largest brown coal mines in Bosnia and Herzegovina (BiH), Kakanj, has been exploited for over a hundred years. As a consequence of decades of exploitation, severe biocenosis disturbance and degradation of the entire ecosystem have occurred, resulting in overburden soil formation. A significant challenge in remediation of degraded mining areas is difficulty in creating conditions favorable for vegetation growth. Thus, numerous remediation technologies have focused on increasing soil nutrient composition, as well as the number and activity of plant growth-promoting bacteria (PGPB), given that they stimulate host plant growth by increasing the availability of essential nutrients (phosphorus, nitrogen, manganese, iron), producing phytohormones, and providing protection from pathogens. The main objective of this research was to characterize autochthonous plant and microbial overburden communities and access their ability to restore these contaminated soils. Phytocenological analysis of vegetation and plant species was performed according to Flora Europaea (2001), from 2011 - 2013. Our results show that plant species were not detected at mine overburden soil in 2011. However, we detected presence of a single plant species, Amaranthus albus L., in 2012. Further, we recorded the presence of five families (Amaranthaceae, Chenopodiaceae, Convolvulaceae, Poaceae and Polygonaceae) in 2013. Microbial abundance and enzymatic activity were also examined during the same period. The diversity of microbial populations in the first year was rather small. Two Bacillus spp., B. simplex and a B. cereus group member, indigenous to mine overburden were isolated and identified using standard macroscopic and microscopic, as well as molecular techniques (Hamidovic et al., submitted). Phosphate solubilizing activity of bacteria was tested on National Botanical Research Institute's phosphate growth medium (1999). Production of ammonia was determined in peptone water with Nessler

  20. Results of microbiological Investigations of Orbital Station MIR Environment

    Science.gov (United States)

    Novikova, N.

    equipment which gather anthropogenic organic compounds and air condensate enough to allow full vegetative cycle and reproduction of heterotrophic microorganisms, mold fungi Penicillium, Aspergillus, Cladosporium sp. in the first place, - quantitative and structural dynamics of microflora on long-operating space vehicles is not linear and presents a wave-form cycle of alternating phases of biocenosis activation and stagnation controlled as by internal biological mechanisms of self-regulation, so by external cosmophysical factors.

  1. Development of Triad approach based system for ecological risk assessment for contaminated areas of Kyrgyzstan

    Science.gov (United States)

    Kydralieva, Kamilia; Uzbekov, Beksultan; Khudaibergenova, Bermet; Terekhova, Vera; Jorobekova, Sharipa

    2014-05-01

    substances), 2) ecological parameters (assessing changes in microorganism's community structure and functions, bioindication); and 3) toxicological bioassays (utilizing classical endpoints such as survival and reproduction rates, genotoxicity). The output will be consisted of 3 indexes: 1) Environmental Risk Index, quantifying the level of biological damage at population-community level, 2) Biological Vulnerability Index, assessing the potential threats to biological equilibria, and 3) Genotoxicity Index, screening genotoxic effects. Multi-criteria Decision Analysis (MCDA) will be used to integrate a set of environmental Triad data to be obtained during the project, which will be carried out in order to estimate the potential risk from soil contamination of the highly anthropized areas of Kadzhi-Say, which have been impacted by deposition of heavy metals. The basis of the development under this research is studies with a particular focus concerning the biocenosis mapping of Kyrgyz soils (Mamytova et al., 2003, 2010), investigations on interaction of humic substances with soil contaminants (Jorobekova, Kydralieva, Khudaibergenova, 2004; Khudaibergenova, 2005, 2007), and in addition, technical approach for ecotoxicological assessment of soils (Terekhova, 2007, 2011). Soil ecotoxicological estimation has been studied with a battery of tests using test-organisms of many trophic levels. Currently, bioindication of soils with various humus states is under study (Senesi, Yakimenko 2007; Yakimenko, et al., 2008).

  2. Reaction of fresh water zooplankton community to chronic radiation exposure

    Energy Technology Data Exchange (ETDEWEB)

    Osipov, D.; Pryakhin, E. [Urals Research Center for Radiation Medicine - URCRM (Russian Federation); Ivanov, I. [FSUE Mayak PA (Russian Federation)

    2014-07-01

    The characteristic features of ecological community as a whole and cenosis of zooplankton organisms as part of it determine the intensity of the processes of self-purification of water and the formation of a particular body of water. Identifying features of the structure and composition of the zooplankton community of aquatic ecosystems exposed to different levels of radiation exposure, it is necessary to identify patterns of changes in zooplankton and hydro-biocenosis as a whole. Industrial reservoirs, the storage of liquid low-level radioactive waste 'Mayak' for decades, have high radiation load. A large range of levels of radioactive contamination (total volume beta-activity in water varies from 2.2x10{sup 3} to 2.3x10{sup 7} Bq/l, total volume alpha-activity - from 2.6x10{sup -1} to 3.1x10{sup 3} Bq/l) provides a unique opportunity to study ecosystems in a number of reservoirs with increasing impact of radiation factor. We studied five reservoirs that were used as the storage of low-and intermediate-level liquid radioactive waste pond and one comparison water body. In parallel with zooplankton sampling water samples were collected for hydro-chemical analysis. 41 indicators were analysed in order to assess the water chemistry. To determine the content of radionuclides in the various components of the ecosystem samples were collected from water, bottom sediments and plankton. Sampling of zooplankton for the quantitative analysis was performed using the method of weighted average auto bathometer. Apshteyn's plankton net of the surface horizon was used for qualitative analysis of the species composition of zooplankton. Software package ERICA Assessment Tool 2012 was used for the calculation of the absorbed dose rate. Species diversity and biomass of zooplankton, the share of rotifers in the number of species, abundance and biomass decrease with the increase of the absorbed dose rate and salinity. The number of species in a sample decreases with the

  3. Microbiological Method for Eor RAH par la méthode microbiologique

    Directory of Open Access Journals (Sweden)

    Yulbarisov E. M.

    2006-11-01

    Full Text Available To ensure a successful development of biotechnology it is necessary to study the processes of oil transformation from its separation from the parent rock followed by maturation and after-maturation periods and then very often aging and destruction (hypergenesis influenced by the tectonic development of the region or the technogenic activity of man. These technogenic actions can be of a multistage character depending on the development system used (natural drive, secondary and tertiary methods of oil recovery. Any change in the properties of the reservoir medium produces a direct or indirect effect on oil properties as well as on the petrophysical pattern of the reservoir capacity and filtration characteristics. Among the factors that affect the desired changes in oil properties and filtration characteristics during microbiological oil displacement, biomass and production of biogas, biosurfactants, biopolymers, acids, solvents, ferments etc. , are of great importance. An effective method for improving oil recovery by commercial biocenosis of microorganisms has been developed. Pour assurer le développement de la biotechnologie, il faut étudier les processus de transformation du pétrole depuis sa séparation de la roche-mère, durant la maturation, la post-maturation, puis souvent le vieillissement et la destruction (hypergenèse sous l'influence de l'évolution tectonique de la région ou des activités technogéniques humaines. Celles-ci peuvent comporter plusieurs étapes, selon le système d'exploitation appliqué (déplacement naturel, méthodes secondaires et tertiaires de récupération. Toute modification des propriétés de la roche-réservoir se répercute directement ou indirectement sur les propriétés du pétrole ainsi que sur les caractéristiques pétrophysiques de capacité et de filtration du réservoir. Parmi les facteurs responsables des transformations souhaitées des propriétés du pétrole et des caractéristiques de

  4. The impact of high commercial fishery load on biological indices of the roach (Rutilus rutilus

    Directory of Open Access Journals (Sweden)

    G. O. Kotovska

    2015-07-01

    Full Text Available Age composition, length and weight indices of the roach populations (Rutilus rutilus L. were analyzed and compared in water bodies with the different commercial fishery loading levels. Presence of fast-growing and slow-growing forms is inherent to cyprinid fish. In Dnieper reservoirs these forms are mixed and it is difficult to separate any one of them. It is assumed that selective elimination of fast-growing forms by commercial fishing may cause accumulation of slow-growing forms in populations. On that ground, water bodies with different levels of commercial fishery pressure have been chosen to test this hypothesis. For instance, Kremenchuk Reservoir was selected as a water body with high level of commercial fishery load because it forms more than a half of roach commercial catching in Ukraine. On the contrary, “Dniprovsko-Orilskiy” Natural Reserve was taken as a water body where human impact is minimum. Subsequently, comparing of the basic biological features of the roach from water bodies with different commercial fishery load illustrated the value of the study. It is found that the roach age range in Kremenchuk Reservoir is much higher than in subordinate waters of the Natural Reserve fund. Namely, the roach population in Kremenchuk Reservoir consisted of seventeen age groups while in “Dniprovsko-Orilskiy” Natural Reserve it comprised ten age groups only. However, size-weight features of species under study across the age groups 6+ –10+ in water bodies with the intensive commercial fishery were statistically lower than in protected waters of the Nature Reserve. Namely, length of these age groups in Kremenchuk Reservoir was equal to 24.6–33.5 cm, compared with 25.0–37.0 cm in the National Reserve. Naturally, that weight was equal to 334–957 and 340–1320 g, respectively. In general, this result does not fit into the traditional concept that the fast-growing roach should inhabit the lacustrine biocenosis of the Dnieper

  5. Researches Concerning Reference Values Assessment of Serum Biochemical Parameters in some Fish Species from Acipenseridae, Cyprinidae, Esocidae and Salmonidae Family

    Directory of Open Access Journals (Sweden)

    Marioara Nicula

    2010-05-01

    Full Text Available The purpose of this work was to assess reference values of serum biochemical indices (enzymes, metabolites and minerals in some representative fish species belonging to Acipenseridae, Cyprinidae, Esocidae and Salmonidae family in order to establish a baseline data which will serve to monitoring nutritional-metabolic balance and healthy condition of these species from aquatic biocenosis or intensive exploitations. Serum samples were analyzed for glucose, total proteins, albumine, urea nitrogen, creatinine, uric acid, triglycerides, cholesterol, ALT, AST, GGT, alkaline phosphatase, amylase, total bilirubin, Ca, P, Mg, Fe, using a FullyVet automated chemical analyser. The obtained results were compared with those from other papers regarding serum biochemical profile of fresh water fish species. Serum biochemical reference intervals were as follows: glucose, 28.41±0.80–64.00±1.41 mgdL-1; total proteins, 2.78±0.21-4.05±0.20 gdL-1; albumine, 0.67±0.12–1.59±0.11 gdL-1; urea nitrogen, 12.16±0.78–18.30±0.27 mgdL-1; creatinine, 0.06±0.01–0.27±0.00 mgdL-1; uric acid, 1.00±0.00–1.66±0.09 mgdL-1; triglycerides, 46.00±1.29– 351.3±12.3 mgdL-1; cholesterol, 123.00±2.12–198.00±0.91±mgdL-1; ALT, 15.00±0.91–32.00±1.29 IU L-1; AST, 30.00±0.91–92.00±1.47 IU L-1; GGT, 4.00±0.00–6.33±0.46 IU L-1; alkaline phosphatase, 60.33±1.20–109.50±3.05 IU L-1; amylase, 28.08±0.93 – 36.00±0.91 IU L-1; total bilirubin, 0.00±0.00–0.03±0.00 mgdL-1; Ca, 7.63±0.40– 12.36±0.50 mgdL-1; P, 11.83±0.35-30.48±0.26 mgdL-1; Mg, 1.80±0.14–3.88±0.21mgdL-1; Fe, 57.60±3.48–120.00± 1.08 gdL-1. The wide intra- and interspecific variability of our data requires subsequent studies of the endo- and exogenous factors (living condition, season, age, gender, origin, breeding system, physiological and nutritional status, genetic of each individual, etc. that can induce variations of the propound parameter.

  6. INVESTIGATION OF ECOLOGICAL FEATURES OF ACUTE DIARRHEAL INFECTION PATHOGENS

    Directory of Open Access Journals (Sweden)

    Malysh N.G.

    2015-12-01

    Full Text Available Introduction. Microbiocenosis of human body also differs in extreme multicomponents and diverse content of microflora representatives forming its part. According to the biotype of bacterial contamination certain inter-bacterial relations are formed, which is reflected in the qualitative and quantitative characteristics of appropriate microbial landscape. Analysis of numerous microbial association manifestations allows evaluating changes in the pathogen properties influenced by associative microbiota. Work objective - based on the study ecological features of microorganisms isolated from intestine of patients with acute intestinal infections and apparently healthy people, identify potential risk factors for diarrheal infections. Materials & methods. A retrospective epidemiological analysis of acute diarrheal infections incidence was conducted during 2004-2013, using the statistics of the Main Department of the State Sanitary and Epidemiological Service of Ukraine in Sumy region. The intestinal microflora of 93 patients with acute diarrheal infections and 60 persons of the control group (apparently healthy people. As the result 130 bacterial cultures were allocated. Permanence rate was used to estimate biocenosis. Relationships between microbiocenosis members were investigated by determining degree of bond conjunction in associations, using Jaccard coefficient (g. Results & discussion. In 2005-2014 acute diarrheal infection incidence rates of Sumy region population were within 163.7 - 193.6 per 100 people without tendency to decrease. Acute intestinal infections and food toxicoinfections caused by opportunistic pathogens and viruses (p<0.05 dominated in nosological structure. In 35.5 % of cases diarrheal infections were of polyetiological nature. Noroviruses in associations with Candida bacteriaand fungi most often occurred (p<0.05 in the intestinal biotypes. Permanence rate of K. pneumonia, noroviruses, S. aureus, C. albicans was the highest and

  7. [Forensic entomology and globalisation].

    Science.gov (United States)

    Turchetto, M; Vanin, S

    2004-06-01

    The main aim of Forensic Entomology has always been, and is today, to establish the time of death (P.M.I.: Postmortem Period) or, more exactly, how long a carrion has been exposed in the environment. Most of the invertebrate fauna occurring on corpses consists of insects (mostly Diptera and Coleoptera). They are selectively attracted by the decomposing status of the carrion, and form complex communities or biocenosis within necrophagous or sarcophagous species and their predators, parasites and parasitoids, competing each one another. The rapid and continuos changes of the micro-ecosystem (the body), until its breakdown, does not permit the achievement of a steady state or an equilibrium in the animal communities. These continuous modifications give us the possibility to estimate when (and where) the death has occurred, by the identification of the species feeding on the corpse, the knowledge of their life history, and the length of each stage of their cycle at varying the temperature and the other abiotic factors, external to the carrion ecosystem. The P.M.I. today is still largely based on the tables of faunal succession on human cadavers recognised by Mégin in 1894, with few changes proposed by Authors from other countries. In the last years, however, it happens more and more often, that the natural communities are subverted by the presence of allocton species, which can compete, predate or parasite the most common local sarcophagous insects, modifying, this way, the succession waves and the trophic nets. The immission in the environment of foreign species may be voluntary or casual, but in any case is due to anthropic activities. The voluntary immission happens when some species, employed in the biological struggle against pest or dangerous insects, for pollination of allocton plants, or for other commercial utilities, are beyond man's control and swarm onto the environment; the casual spread is due to the globalisation phenomenon, that distributes the "little

  8. Microbiome of Yermic Regosol in southern Kazakhstan

    Science.gov (United States)

    Kutovaya, Olga; Lebedeva, Marina; Tkhakakhova, Azida

    2014-05-01

    Biological activity is of utmost importance for the genesis of extremely arid desert soils. The soil surface in arid regions is often covered by biofilms representing a complex biocenosis of algae, bacteria, micromycetes, and, sometimes, mosses or lichens. Biofilms of extremely arid soils form a significant part of the living matter in the desert ecosystems and play the central role in their dynamics. Study of the genetic material recovered directly from the soil samples is the main approach in soil metagenomics. Modern sequencing methods were used to describe the diversity of the microorganisms in soil samples. For the first time, such data were obtained for the extremely arid desert soil (Yermic Regosol) in southern Kazakhstan (flat alluvial plain; 43° 42'53.2" N; 79°25'29.1" E; 615 m asl). Taxonomic identification of nucleotide sequences and comparative analysis of microbial communities were performed using VAMPS. The classification of the sequences was performed using RDP. As the primers used were based on the sequences of 16S-rRNA gene of bacteria and archaea, we could analyze the prokaryotic community. Along with bacteria and archaea with established systematic position, all soil samples contained unidentified sequences (5.2-5.3%). Bacteria predominated at the domain level (65.9-74.9%), although their portion was much lower in comparison with that in less arid soils, where it reached 94-100%. Archaea were present as minor components (0.3-0.5%). Dominant groups of bacteria were represented by Proteobacteria (43.9-50.8%), Actinobacteria (9.5-10%), Firmicutes (0.8-2.4%), Verrucomicrobia (1.1-3%), Acidobacteria (1.1-2%), Bacteroidetes (1.2-1.4%). The portion of other phyla was less than 1%. Thus, bacterial phyla Proteobacteria, Actinobacteria and Acidobacteria constitute the core component of the microbiome. Archaea are represented by phylum Crenarchaeota. A key feature of the extremely arid soils is the presence of large numbers (24.7-33.6%) of cyanobacteria

  9. Linee guida per la gestione della Volpe in Italia

    Directory of Open Access Journals (Sweden)

    Silvano Toso

    2003-10-01

    Full Text Available La plasticità ecologica della Volpe (Vulpes vulpes fa di questa specie il Carnivoro più diffuso e più adattabile al mondo. Proprio per la sua diffusione la Volpe è oggetto di un intenso prelievo sia venatorio, sia finalizzato a contenerne l'impatto predatorio ed allo sfruttamento commerciale della pelliccia. In Italia esiste una forte pressione dei cacciatori perché si realizzino programmi di controllo delle popolazioni di questo predatore, al fine di ridurne l'impatto sulle specie d'interesse cinegetico. Gli effetti del contenimento della Volpe sulla dinamica delle popolazioni di specie preda sono stati oggetto di numerosi studi, ma i risultati di tali ricerche non sono univoci; se in alcuni casi si è infatti verificato, in seguito a decrementi delle popolazioni di Volpe, un incremento di consistenza e successo riproduttivo delle specie preda, in altri casi non si è registrato alcun significativo effetto demografico. Ancora meno chiaro è quale sia la soglia di contrazione delle popolazioni di Volpe necessaria per ottenere un effetto significativo sulle popolazioni delle specie preda, considerato che questo Carnivoro ha un potenziale riproduttivo elevato ed un'organizzazione sociale che determina la rapida rioccupazione dei territori lasciati liberi. La legislazione italiana in materia di controllo è, rispetto ad altri paesi europei ed extraeuropei, particolarmente rigida: la legge n. 157/92 identifica infatti un obiettivo generale di mantenimento delle popolazioni dei predatori a livelli adeguati ad assicurare una funzione regolatrice delle biocenosi. La stessa norma impone inoltre che ogni intervento di abbattimento di predatori finalizzato al contenimento dei danni da essi arrecati sia autorizzato dalla Regione o dalla Provincia competente, sulla base di un parere tecnico dell'INFS che è chiamato a verificare l'inapplicabilità di metodi incruenti di prevenzione dei danni, la selettività delle tecniche di abbattimento e la

  10. Inspiring our future citizens and scientists: follow the Blue Paths (Percorsi nel Blu)!

    Science.gov (United States)

    Mioni, Erika; Stroobant, Mascha; Merlino, Silvia; Traverso, Roberto

    2016-04-01

    project of Biological Sciences, carried out in a pilot school of La Spezia (ISA 2 "2 Giugno"), and that since several years involves students aged 4 to 18 years (and their families) in a long-term training program for environmental awareness and marine scientific research. The project is aimed at monitoring marine biocenosis (flora and fauna) from the upper and middle shore, using and integrating the Reef-Check Protocol (C.E.M. Coastal Environment Monitoring Protocol) with a new approach that motivates students to science, continuously over the years and bringing them closer to the work of Marine Scientists, raising awareness on biodiversity loss and the pollution within a specific area such as that of the Pelagos Sanctuary in the North Tyrrhenian and Ligurian Seas. Blue Paths (Percorsi nel Blu) is, hence, an example of education with a wider communication spectrum (not focused only to form a single class of students, but a whole community) and made possible thanks to a network of partners (including technology clusters, Institutions, Museums, Research Centers, Marine Protected Areas etc.). The presence of the network has allowed the enhancement of the Project under different points of view, including: - transformation in a participatory research project (the scientific results obtained by students are real products of research) of citizen science; - increase of educational opportunities (peer education, intergenerational education, tutoring); - increased visibility thanks to the participation in European projects for the Science Communication (European Researchers' Night project funded by the European Commission under the Marie Sklodowska-Curie actions); - enrichment of students' curriculum thanks to an adequate and real acquisition of key skills for future careers that can also inspire their actions as future citizens.

  11. Analysis of active microorganisms and their potential role in carbon dioxide turnover in the natural gas reservoirs Altmark and Schneeren (Germany)

    Science.gov (United States)

    Gniese, Claudia; Muschalle, Thomas; Mühling, Martin; Frerichs, Janin; Krüger, Martin; Kassahun, Andrea; Seifert, Jana; Hoth, Nils

    2010-05-01

    RECOBIO-2, part of the BMBF-funded Geotechnologien consortium, investigates the presence of active microorganisms and their potential role in CO2 turnover in the formation waters of the Schneeren and Altmark gas fields, which are both operated by GDF SUEZ E&P Germany GmbH. Located to the north west of Hannover the natural gas reservoir Schneeren is composed of compacted Westfal-C sandstones that have been naturally fractured into a subsalinar horst structure. This gas field is characterized by a depth of 2700 to 3500m, a bottom-hole temperature between 80 and 110° C as well as a moderate salinity (30-60g/l) and high sulfate contents (~1000mg/l). During RECOBIO-1 produced formation water collected at wells in Schneeren was already used to conduct long term laboratory experiments. These served to examine possible microbial processes of the autochthonous biocenosis induced by the injection of CO2 (Ehinger et al. 2009 submitted). Microorganisms in particular sulfate-reducing bacteria and methanogens were able to grow in the presence of powdered rock material, CO2 and H2 without any other added nutrients. The observed development of DOC was now proven in another long term experiment using labelled 13CO2. In contrast to Schneeren, the almost depleted natural gas reservoir Altmark exhibits an average depth of 3300m, a higher bottom-hole temperature (111° C to 120° C), a higher salinity (275-350g/l) but sulfate is absent. This Rotliegend formation is located in the southern edge of the Northeast German Basin and is of special interest for CO2 injection because of favourable geological properties. Using molecular biological techniques two types of samples are analyzed: formation water collected at the well head (November 2008) and formation water sampled in situ from a depth of around 3000m (May 2009). Some of the wells are treated frequently with a foaming agent while others are chemically untreated. Despite the extreme environmental conditions in the Altmark gas field

  12. Agro-ecological compensation standard based on emergy analysis in Yongding River basin%基于能值分析的永定河流域农业生态补偿标准

    Institute of Scientific and Technical Information of China (English)

    付意成; 高婷; 闫丽娟; 张爱静; 阮本清

    2013-01-01

    the paper indicates that by the adoption of the emergy accounting method, the government and producers would be responsible for a portion of the PES, and the emprice calculated for the environmental support and the environmental benefits of farmland should be used to decide who will bear the responsibility for making payments for environmental services. To realize the sustainable development of basin agriculture, the government and beneficiary sectors will compensate farmers soil loss preventive costs 400 million Yuan. The compensation standard calculation method of agro-ecological system in the light of emergy is feasible. Further studies are necessary on agricultural production environmental services, such as the maintenance of the biodiversity and natural biocenosis, and hydrological services.

  13. MICROECOLOGY OF THE MUCOUS MEMBRANE OF THE ALVEOLAR RIDGES IN THE PERIOD OF ADAPTATION TO COMPLETE DENTURES

    Directory of Open Access Journals (Sweden)

    Yanishen I. V.,

    2016-06-01

    quantitative composition of the biocenosis. It is established that the microflora of the alveolar ridge in patients with edentulous consisted of associations of yeasts from 2-5 representatives of the microbial world. It is established that in patients of the experimental group on the 7th day of withdrawal 3-component microbial associations has decreased in 1.3 times, whereas the 4-component associations were recorded in 1.4 times more often compared with the initial period of observation. For patients with adhesive frequency of detection of 2-component microbial associations 30-the day of the research was 1.3 times higher than prior to the setting of the prosthesis. Identify 5 component associations for 30-the day, has decreased in 2 times. In patients without adhesive (control group 4-component microbial associations in the mouth after a week of adaptation to the prosthesis were detected in 1.6 times, and 30 days – in 2 times more often than to prosthetics. The weight 5 component associations through the week remained at the initial level, while after 30 days has increased in 1,5 times. The structure microbiocenosis the mucous of the oral cavity patients with edentulism are represented 13 genera of bacteria and morilioid fungi of the genus Candida, allocated in secondary amounts by lg (2,5±0,19 to lg (5,4±0,17 CFU/g. When adhesive between denture base and mucosa of the alveolar ridge frequency of withdrawal and the density of microbial colonization in the adaptation period were not statistically different. But the density of the microbial population among the control group increased 1.5 times for Enterococcus spp, 1.4 for the Klebsiella spp and 1.6 times for yeast fungi Candida spp. Found a significant decrease in microbial density of the representatives of the resident microflora in 1.4 times for Neisseria spp, 1.6-fold for Lactobacillus spp (p<0.05. Conclusion. The results of these studies indicate significant changes of qualitative and quantitative structure of