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Sample records for bilirubin

  1. Bilirubin - urine

    Science.gov (United States)

    Conjugated bilirubin - urine; Direct bilirubin - urine ... Bilirubin is not normally found in the urine. ... Increased levels of bilirubin in the urine may be due to: Biliary tract disease Cirrhosis Gallstones in the biliary tract Hepatitis Liver disease ...

  2. Bilirubin Test

    Science.gov (United States)

    ... than females. African Americans routinely show lower bilirubin concentrations than non-African Americans. Strenuous exercise may increase bilirubin levels. Drugs that can decrease total bilirubin include barbiturates, caffeine, penicillin, and high doses of salicylates. The drug ...

  3. Blood Test: Bilirubin

    Science.gov (United States)

    ... Your 1- to 2-Year-Old Blood Test: Bilirubin KidsHealth > For Parents > Blood Test: Bilirubin A A A What's in this article? What ... Análisis de sangre: bilirrubina What It Is A bilirubin test measures the level of bilirubin (a byproduct ...

  4. Biology of Bilirubin Photoisomers.

    Science.gov (United States)

    Hansen, Thor Willy Ruud

    2016-06-01

    Phototherapy is the main treatment for neonatal hyperbilirubinemia. In acute treatment of extreme hyperbilirubinemia, intensive phototherapy may have a role in 'detoxifying' the bilirubin molecule to more polar photoisomers, which should be less prone to crossing the blood-brain barrier, providing a 'brain-sparing' effect. This article reviews the biology of bilirubin isomers. Although there is evidence supporting the lower toxicity of bilirubin photoisomers, there are studies showing the opposite. There are methodologic weaknesses in most studies and better-designed experiments are needed. In an infant acutely threatened by bilirubin-induced brain damage, intensified phototherapy should be used expediently and aggressively.

  5. Bilirubin oxidation in brain.

    Science.gov (United States)

    Hansen, T W

    2000-01-01

    Bilirubin is a product of heme catabolism which by virtue of its lipid solubility can cross the blood-brain barrier and enter the brain. Neonatal jaundice is a common transitional phenomenon which is due to the combination of increased heme catabolism and rate limitations as far as hepatic conjugation and biliary excretion of bilirubin. In the great majority of cases this is an innocuous condition, which is even posited to have some beneficial effects due to the ability of bilirubin to quench free oxygen radicals. However, because bilirubin is neurotoxic, hyperbilirubinemia in the newborn may exceptionally result in death in the neonatal period, or survival with severe neurological sequelae (kernicterus). Bilirubin enters the brain through an intact blood-brain barrier. Clearance of bilirubin from brain partly involves retro-transfer through the blood-brain barrier, and possibly also through the brain-CSF barrier into CSF. Work in our lab during the past 5 years has substantiated earlier work which had suggested that bilirubin may also be metabolized in brain. The responsible enzyme is found on the inner mitochondrial membrane, and oxidizes bilirubin at a rate of 100-300 pmol bilirubin/mg protein/minute. The enzyme activity is lower in the newborn compared with the mature animal, and is also lower in neurons compared with glia. Studies of different rat strains have documented genetic variability. The enzyme is cytochrome-c-dependent, but has as yet not been unequivocally identified. The rate of oxidation of bilirubin is such that this enzyme probably contributes meaningfully to the clearance of bilirubin from brain.

  6. Bilirubin measurements in neonates

    Science.gov (United States)

    Newman, Gregory J.

    2000-04-01

    Infant Jaundice is a physiologic condition of elevated bilirubin in the tissue that affects nearly 60 percent of all term newborns and virtually 100 percent of premature infants. The high production of bilirubin in the newborn circulatory system and the inability of the immature liver to process and eliminate it case the condition. When the bilirubin levels rise, it starts to deposit in the baby's skin and in the brain. The deposits in the brain can cause neurologic impairment and death. The BiliCheck is a handheld, battery-powered device that measures the level of jaundice non-invasively using BioPhotonics at the point of care. The result is displayed on an LCD screen immediately, so physicians can now make treatment decision without waiting for results to return from the lab. The BiliCheck System has been marketed worldwide since April of 1998 and has received FDA clearance for use in the USA on pre-photo therapy infants in March of 1999.

  7. Bilirubin-albumin binding, bilirubin/albumin ratios, and free bilirubin levels : Where do we stand?

    NARCIS (Netherlands)

    Hulzebos, Christian V.; Dijk, Peter H.

    2014-01-01

    Treatment for unconjugated hyperbilirubinemia is predominantly based on one parameter, i.e., total serum bilirubin (TSB) levels. Yet, overt kernicterus has been reported in preterm infants at relatively low TSB levels, and it has been repeatedly shown that free unconjugated bilirubin (freeUCB) level

  8. The Biological Effects of Bilirubin Photoisomers.

    Science.gov (United States)

    Jasprova, Jana; Dal Ben, Matteo; Vianello, Eleonora; Goncharova, Iryna; Urbanova, Marie; Vyroubalova, Karolina; Gazzin, Silvia; Tiribelli, Claudio; Sticha, Martin; Cerna, Marcela; Vitek, Libor

    2016-01-01

    Although phototherapy was introduced as early as 1950's, the potential biological effects of bilirubin photoisomers (PI) generated during phototherapy remain unclear. The aim of our study was to isolate bilirubin PI in their pure forms and to assess their biological effects in vitro. The three major bilirubin PI (ZE- and EZ-bilirubin and Z-lumirubin) were prepared by photo-irradiation of unconjugated bilirubin. The individual photoproducts were chromatographically separated (TLC, HPLC), and their identities verified by mass spectrometry. The role of Z-lumirubin (the principle bilirubin PI) on the dissociation of bilirubin from albumin was tested by several methods: peroxidase, fluorescence quenching, and circular dichroism. The biological effects of major bilirubin PI (cell viability, expression of selected genes, cell cycle progression) were tested on the SH-SY5Y human neuroblastoma cell line. Lumirubin was found to have a binding site on human serum albumin, in the subdomain IB (or at a close distance to it); and thus, different from that of bilirubin. Its binding constant to albumin was much lower when compared with bilirubin, and lumirubin did not affect the level of unbound bilirubin (Bf). Compared to unconjugated bilirubin, bilirubin PI did not have any effect on either SH-SY5Y cell viability, the expression of genes involved in bilirubin metabolism or cell cycle progression, nor in modulation of the cell cycle phase. The principle bilirubin PI do not interfere with bilirubin albumin binding, and do not exert any toxic effect on human neuroblastoma cells.

  9. Quantitative imaging of bilirubin by photoacoustic microscopy

    Science.gov (United States)

    Zhou, Yong; Zhang, Chi; Yao, Da-Kang; Wang, Lihong V.

    2013-03-01

    Noninvasive detection of both bilirubin concentration and its distribution is important for disease diagnosis. Here we implemented photoacoustic microscopy (PAM) to detect bilirubin distribution. We first demonstrate that our PAM system can measure the absorption spectra of bilirubin and blood. We also image bilirubin distributions in tissuemimicking samples, both without and with blood mixed. Our results show that PAM has the potential to quantitatively image bilirubin in vivo for clinical applications.

  10. Bilirubin-albumin binding, bilirubin/albumin ratios, and free bilirubin levels: where do we stand?

    Science.gov (United States)

    Hulzebos, Christian V; Dijk, Peter H

    2014-11-01

    Treatment for unconjugated hyperbilirubinemia is predominantly based on one parameter, i.e., total serum bilirubin (TSB) levels. Yet, overt kernicterus has been reported in preterm infants at relatively low TSB levels, and it has been repeatedly shown that free unconjugated bilirubin (freeUCB) levels, or bilirubin/albumin (B/A) ratios for that matter, are more closely associated with bilirubin neurotoxicity. In this article, we review bilirubin-albumin binding, UCBfree levels, and B/A ratios in addition to TSB levels to individualize and optimize treatment especially in preterm infants. Methods to measure bilirubin-albumin binding or UCBfree are neither routinely performed in Western clinical laboratories nor incorporated in current management guidelines on unconjugated hyperbilirubinemia. For bilirubin-albumin binding, this seems justified because several of these methods have been challenged, and sufficiently powered prospective trials on the clinical benefits are lacking. Technological advances in the measurement of UCBfree may provide a convenient means for integrating UCBfree measurements into routine clinical management of jaundiced infants. A point-of-care method, as well as determination of UCBfree levels in various newborn populations, is desirable to learn more about variations in time and how various clinical pathophysiological conditions affect UCBfree levels. This will improve the estimation of approximate UCBfree levels associated with neurotoxicity. To delineate the role of UCBfree in the management of jaundiced (preterm) infants, trials are needed using UCBfree as treatment parameter. The additional use of the B/A ratio in jaundiced preterms has been evaluated in the Bilirubin Albumin Ratio Trial (BARTrial; Clinical Trials: ISRCTN74465643) but failed to demonstrate better neurodevelopmental outcome in preterm infants bilirubin toxicity. Yet, different methodologies for estimating these parameters exist, and sufficiently powered, prospective clinical

  11. Photoacoustic microscopy of bilirubin in tissue phantoms

    Science.gov (United States)

    Zhou, Yong; Zhang, Chi; Yao, Da-Kang; Wang, Lihong V.

    2012-12-01

    Determining both bilirubin's concentration and its spatial distribution are important in disease diagnosis. Here, for the first time, we applied quantitative multiwavelength photoacoustic microscopy (PAM) to detect bilirubin concentration and distribution simultaneously. By measuring tissue-mimicking phantoms with different bilirubin concentrations, we showed that the root-mean-square error of prediction has reached 0.52 and 0.83 mg/dL for pure bilirubin and for blood-mixed bilirubin detection (with 100% oxygen saturation), respectively. We further demonstrated the capability of the PAM system to image bilirubin distribution both with and without blood. Finally, by underlaying bilirubin phantoms with mouse skins, we showed that bilirubin can be imaged with consistent accuracy down to >400 μm in depth. Our results show that PAM has potential for noninvasive bilirubin monitoring in vivo, as well as for further clinical applications.

  12. The Biological Effects of Bilirubin Photoisomers.

    Directory of Open Access Journals (Sweden)

    Jana Jasprova

    Full Text Available Although phototherapy was introduced as early as 1950's, the potential biological effects of bilirubin photoisomers (PI generated during phototherapy remain unclear. The aim of our study was to isolate bilirubin PI in their pure forms and to assess their biological effects in vitro. The three major bilirubin PI (ZE- and EZ-bilirubin and Z-lumirubin were prepared by photo-irradiation of unconjugated bilirubin. The individual photoproducts were chromatographically separated (TLC, HPLC, and their identities verified by mass spectrometry. The role of Z-lumirubin (the principle bilirubin PI on the dissociation of bilirubin from albumin was tested by several methods: peroxidase, fluorescence quenching, and circular dichroism. The biological effects of major bilirubin PI (cell viability, expression of selected genes, cell cycle progression were tested on the SH-SY5Y human neuroblastoma cell line. Lumirubin was found to have a binding site on human serum albumin, in the subdomain IB (or at a close distance to it; and thus, different from that of bilirubin. Its binding constant to albumin was much lower when compared with bilirubin, and lumirubin did not affect the level of unbound bilirubin (Bf. Compared to unconjugated bilirubin, bilirubin PI did not have any effect on either SH-SY5Y cell viability, the expression of genes involved in bilirubin metabolism or cell cycle progression, nor in modulation of the cell cycle phase. The principle bilirubin PI do not interfere with bilirubin albumin binding, and do not exert any toxic effect on human neuroblastoma cells.

  13. Bilirubin Binding to PPARα Inhibits Lipid Accumulation.

    Science.gov (United States)

    Stec, David E; John, Kezia; Trabbic, Christopher J; Luniwal, Amarjit; Hankins, Michael W; Baum, Justin; Hinds, Terry D

    2016-01-01

    Numerous clinical and population studies have demonstrated that increased serum bilirubin levels protect against cardiovascular and metabolic diseases such as obesity and diabetes. Bilirubin is a potent antioxidant, and the beneficial actions of moderate increases in plasma bilirubin have been thought to be due to the antioxidant effects of this bile pigment. In the present study, we found that bilirubin has a new function as a ligand for PPARα. We show that bilirubin can bind directly to PPARα and increase transcriptional activity. When we compared biliverdin, the precursor to bilirubin, on PPARα transcriptional activation to known PPARα ligands, WY 14,643 and fenofibrate, it showed that fenofibrate and biliverdin have similar activation properties. Treatment of 3T3-L1 adipocytes with biliverdin suppressed lipid accumulation and upregulated PPARα target genes. We treated wild-type and PPARα KO mice on a high fat diet with fenofibrate or bilirubin for seven days and found that both signal through PPARα dependent mechanisms. Furthermore, the effect of bilirubin on lowering glucose and reducing body fat percentage was blunted in PPARα KO mice. These data demonstrate a new function for bilirubin as an agonist of PPARα, which mediates the protection from adiposity afforded by moderate increases in bilirubin.

  14. Usefulness of the bilirubin/albumin ratio for predicting bilirubin-induced neurotoxicity in premature infants

    NARCIS (Netherlands)

    Hulzebos, C. V.; van Imhoff, D. E.; Bos, A. F.; Ahlfors, C. E.; Verkade, H. J.; Dijk, P. H.

    2008-01-01

    Unconjugated hyperbilirubinaemia occurs in almost all premature infants and is potentially neurotoxic. Treatment is based on total serum bilirubin (TSB), but treatment thresholds are not evidence based. Free bilirubin (Bf) - that is, not bound to albumin, seems a better parameter for bilirubin neuro

  15. Functionalized SBA-15 materials for bilirubin adsorption

    Science.gov (United States)

    Tang, Tao; Zhao, Yanling; Xu, Yao; Wu, Dong; Xu, Jun; Deng, Feng

    2011-05-01

    To investigate the driving force for bilirubin adsorption on mesoporous materials, a comparative study was carried out between pure siliceous SBA-15 and three functionalized SBA-15 mesoporous materials: CH 3-SBA-15 (MS), NH 2-SBA-15 (AS), and CH 3/NH 2-SBA-15 (AMS) that were synthesized by one-pot method. The obtained materials exhibited large surface areas (553-810 m 2/g) and pore size (6.6-7.1 nm) demonstrated by XRD and N 2-ad/desorption analysis. The SEM images showed that the materials had similar fiberlike morphology. The functionalization extent was calculated according to 29Si MAS NMR spectra and it was close to the designed value (10%). The synthesized mesoporous materials were used as bilirubin adsorbents and showed higher bilirubin adsorption capacities than the commercial active carbon. The adsorption capacities of amine functionalized samples AMS and AS were larger than those of pure siliceous SBA-15 and MS, indicating that electrostatic interaction was the dominant driving force for bilirubin adsorption on mesoporous materials. Increasing the ionic strength of bilirubin solution by adding NaCl would decrease the bilirubin adsorption capacity of mesoporous material, which further demonstrated that the electrostatic interaction was the dominant driving force for bilirubin adsorption. In addition, the hydrophobic interaction provided by methyl groups could promote the bilirubin adsorption.

  16. Bilirubin Oxidase Activity of Bacillus subtilis CotA

    OpenAIRE

    Sakasegawa, S; Ishikawa, H.; Imamura, S.; Sakuraba, H.; Goda, S.; Ohshima, T.

    2006-01-01

    The spore coat protein CotA from Bacillus subtilis was previously identified as a laccase. We have now found that CotA also shows strong bilirubin oxidase activity and markedly higher affinity for bilirubin than conventional bilirubin oxidase. This is the first characterization of bilirubin oxidase activity in a bacterial protein.

  17. Intermolecular interactions in the bilirubin-cholate-silica system

    Science.gov (United States)

    Vlasova, N. N.; Golovkova, L. P.; Severinovskaya, O. V.

    2007-06-01

    Bilirubin-cholate interactions in aqueous solutions were studied. The constants of binding of bilirubin with taurocholate dimers and taurodeoxycholate trimers were calculated. The adsorption of bilirubin and cholates on the surface of highly dispersed silica was studied. It was shown that taurine-conjugated cholates are poorly adsorbed from micellar solutions on the silica surface, the specific amount of bilirubin adsorbed decreases with increasing concentration of cholates in the solution, the affinity of free bilirubin for the silica surface is independent of the nature of the cholic acid, and that the affinity of cholate-bilirubin complexes for the silica surface is lower than the affinity of free bilirubin.

  18. Does bilirubin protect against developing diabetes mellitus?

    Science.gov (United States)

    Breimer, Lars H; Mikhailidis, Dimitri P

    2016-01-01

    After 25 years of evaluating bilirubin as a possible protective agent in neonatal and cardiovascular disease, interest has moved on to a exploring a possible protective role in diabetes mellitus (DM). This review finds conflicting prospective data for a protective relationship though there are retrospective, case-controlled data, that can only show association, which is not causality. Only prospective studies can show causality. Also, it would appear that the underlying biochemical assumptions do not readily translate from the animal to the human setting. Given that many factors impact on circulating bilirubin levels, it is not surprising that a clear-cut answer is not available; the jury is still out. Any relationship between DM and bilirubin might relate to intermediates in bilirubin metabolism, including relationships involving the genes for the enzymes participating in those steps. Nevertheless, the pursuit of bilirubin in disease causation is opening new avenues for research and if it is established that serum bilirubin can predict risks, much will have been achieved. The answer may have to come from molecular genetic analyses.

  19. Supramolecular Complexes Formed in Systems Bile Salt-Bilirubin-Silica

    Science.gov (United States)

    Vlasova, N. N.; Severinovskaya, O. V.; Golovkova, L. P.

    The formation of supramolecular complexes between bilirubin and primary micelles of bile salts has been studied. The association constants of bile salts and binding of bilirubin with these associates have been determined. The adsorption of bilirubin and bile salts from individual and mixed aqueous solutions onto hydrophobic silica surfaces has been investigated. The interaction of bilirubin with primary bile salt micelles and the strong retention in mixed micelles, which are supramolecular complexes, result in the adsorption of bilirubin in free state only.

  20. Inaccurate values for direct bilirubin with some commonly used direct bilirubin procedures.

    Science.gov (United States)

    Chan, K M; Scott, M G; Wu, T W; Clouse, R E; Calvin, D R; Koenig, J; Lichti, D A; Ladenson, J H

    1985-09-01

    We compared five methods for the determination of total and direct bilirubins in serum samples from normal controls, subjects with Gilbert's syndrome, and serum pools containing about 50 and 150 mg of total bilirubin per liter. The Kodak Ektachem method and a diazotized sulfanilic acid method with 0.15 mmol/L sodium nitrite concentrations are the only methods that gave accurate direct bilirubin values, as judged by liquid-chromatographic results. The aca method that involved p-nitrobenzene diazonium tetrafluoroborate and another diazotized sulfanilic acid method with a higher concentration of sodium nitrite (0.8 mmol/L) yielded falsely high values for direct bilirubin, which could lead to clinical confusion. The more recently introduced diazotized sulfanilic acid method of the aca gave substantially better results than the p-nitrobenzene diazonium tetrafluoroborate method but was still inaccurate. Systematic investigation of these procedures revealed that the overestimation of direct bilirubin by the diazotized sulfanilic acid method was related to the amount of unconjugated bilirubin present and its ability to react as direct bilirubin in the presence of higher concentrations of sodium nitrite. Inherent properties of p-nitrobenzene diazonium tetrafluoroborate appeared to be responsible for inaccuracies in that method, which could not be corrected by varying reagent concentration or the reaction conditions.

  1. Can Excess Bilirubin Levels Cause Learning Difficulties?

    Science.gov (United States)

    Pretorius, E.; Naude, H.; Becker, P. J.

    2002-01-01

    Examined learning problems in South African sample of 7- to 14-year-olds whose mothers reported excessively high infant bilirubin shortly after the child's birth. Found that this sample had lowered verbal ability with the majority also showing impaired short-term and long-term memory. Findings suggested that impaired formation of astrocytes…

  2. BILIRUBIN AS AN INDIRECT MEASURE OF LABORATORY PERFORMANCE OF BILIRUBIN DETERMINATIONS

    Directory of Open Access Journals (Sweden)

    Suresh Babu

    2015-09-01

    Full Text Available OBJECTIVE : To correlate Total Serum Bilirubin (TSB values to the clinical course of hyperbilirubinemia in newborns as this can be an indirect method of quality assurance in the laboratory. METHODS : An observational study of bilirubin values from 100 randomly selected case records of newborn jaundice for a period of 6 months. TSB values were determined by diazo reaction on venous blood samples on a semi auto analyzer. MS excel sheet used for statistical analysis. RESULTS : Clinical course of hyperbilirubinemia in all subjects correlated well to the reported TSB values in first to last zones corresponding to 15 mg /dL on 3rd day to 5th day of age. Zones 3, 4 and 5 varied from 7th day of birth, as phototherapy and recovery altered visual assessment of jaundice. One patient was expired with kernicterus had very high TSB value. The median bilirubin values trend downfall which correlated clinically to recovery from jaundice and 33% rapid decline in TSB also indicated the intervention by phototherapy. CONCLUSIONS : Bilirubin is one parameter with higher inter laboratory variability since its discovery till today. Hence more quality methods are to be developed to minimize this bias in clinical interpretation of reported bilirubin levels. Our study is an intermediary quality measure useful for both clinicians and lab personnel. This study can be adopted for retrospective quality evaluation and can be adopted for other parameters as well.

  3. Studies on bile acid and bilirubin in liver diseases Part 2. Clinical significance of serum bilirubin sulfate in various liver diseases

    OpenAIRE

    石川, 泰祐

    1980-01-01

    The clinical significance of serum bilirubin sulfate, one of the direct bilirubin, was evaluated in various liver diseases with over 2 mg/dl of serum bilirubin concentration. The diagnosis included 25 cases of acute hepatitis, 8 cases of chronic hepatitis, 8 cases of liver cirrhosis and 16 cases of liver cirrhosis with hepatoma. Bilirubin sulfate was fractioned by Yonei's solvent partition method. The clinical significance of bilirubin sulfate was assessed by comparison of bilirubin sulfate w...

  4. Orlistat treatment increases fecal bilirubin excretion and decreases plasma bilirubin concentrations in hyperbilirubinemic Gunn rats

    NARCIS (Netherlands)

    Nishioka, T; Hafkamp, AM; Havinga, R; Van Lierop, PPE; Velvis, H; Verkade, HJ

    2003-01-01

    Objective To determine whether serum levels of unconjugated bilirubin (UCB) can be decreased by enhancing fecal fat excretion. Study design Gunn rats were fed a high-fat diet (control) or the same diet mixed with the lipase inhibitor orlistat. At regular intervals, plasma UCB concentrations were det

  5. Bilirubin dosage in cord blood: could it predict neonatal hyperbilirubinemia?

    Directory of Open Access Journals (Sweden)

    Adélia Jeha Nasser Bernaldo

    Full Text Available CONTEXT: With early discharge, many newborns have to be readmitted to hospital for hyperbilirubinemia to be treated, and this has been held responsible for the reappearance of kernicterus. OBJECTIVE: To evaluate whether bilirubin levels in cord blood could predict neonatal hyperbilirubinemia that would require treatment, in full-term newborns up to their third day of life. TYPE OF STUDY: Prospective study. SETTING: Neonatal Unit of Hospital Israelita Albert Einstein, São Paulo, Brazil. PARTICIPANTS: 380 full-term newborns considered normal: with or without ABO/Rh blood group incompatibility and without other complications. PROCEDURES: Blood was taken from the umbilical cord for analysis of conjugated, unconjugated and total bilirubin serum levels. The newborns were followed up until discharge, and unconjugated bilirubin that required phototherapy was compared to the cord bilirubin assay. Discriminant analysis was used to classify newborns: with or without risk of needing phototherapy by the third day of life. MAIN MEASUREMENTS: Bilirubin assay in cord blood; mother's and newborn's blood groups; phototherapy indication. RESULTS: The mean value for unconjugated bilirubin in cord blood was significantly higher in newborns whose unconjugated bilirubin required phototherapy. The presence of ABO blood group incompatibility was a significant variable in relation to unconjugated bilirubin that required phototherapy. The most useful cutoff point for unconjugated bilirubin in cord blood was 2.0 mg/100 ml. DISCUSSION: Cord blood could be collected, stored and used for further analysis of unconjugated bilirubin levels as a means for considering whether or not to discharge a moderately jaundiced child from hospital, in association with other resources. CONCLUSIONS: Blood incompatibility between mother and child was a predictor for the appearance of hyperbilirubinemia that required treatment. Considering a cutoff point of 2.0 mg/100 ml, it could be concluded

  6. The inverse association of incident cardiovascular disease with plasma bilirubin is unaffected by adiponectin

    NARCIS (Netherlands)

    Dullaart, Robin P. F.; Boersema, Jeltje; Lefrandt, Joop D.; Wolffenbuttel, Bruce H. R.; Bakker, Stephan J. L.

    2014-01-01

    Objective: Bilirubin may protect against atherosclerotic cardiovascular disease (CVD). The heme oxygenase pathway is crucial for bilirubin generation, and is stimulated by adiponectin. We tested the relationship of plasma bilirubin with adiponectin, and determined whether the association of incident

  7. Low-normal free thyroxine confers decreased serum bilirubin in type 2 diabetes mellitus

    NARCIS (Netherlands)

    Deetman, Petronella E.; Kwakernaak, Arjan J.; Bakker, Stephan J. L.; Dullaart, Robin P. F.

    2013-01-01

    Background: Bilirubin may confer cardiovascular protection because of its strong antioxidative properties. Both thyroid dysfunction and the diabetic state affect bilirubin metabolism. Here we tested whether low-normal thyroid function affects serum bilirubin among euthyroid subjects with and without

  8. Extreme Bilirubin Levels as a Causal Risk Factor for Symptomatic Gallstone Disease

    DEFF Research Database (Denmark)

    Stender, Stefan; Frikke-Schmidt, Ruth; Nordestgaard, Børge G

    2013-01-01

    In individuals without blockage of their bile ducts, levels of plasma bilirubin likely reflect levels of biliary bilirubin; higher biliary bilirubin levels may increase the risk of gallstone disease....

  9. Genetically elevated bilirubin and risk of ischaemic heart disease

    DEFF Research Database (Denmark)

    Stender, Stefan; Frikke-Schmidt, R; Nordestgaard, B G

    2013-01-01

    Elevated plasma levels of bilirubin, an endogenous antioxidant, have been associated with reduced risk of ischaemic heart disease (IHD) and myocardial infarction (MI). Whether this is a causal relationship remains unclear.......Elevated plasma levels of bilirubin, an endogenous antioxidant, have been associated with reduced risk of ischaemic heart disease (IHD) and myocardial infarction (MI). Whether this is a causal relationship remains unclear....

  10. Does bilirubin protect against hemochromatosis gene (HFE) related mortality?

    NARCIS (Netherlands)

    Alizadeh, Behrooz Z.; Njajou, Omer T.; Houwing-Duistermaat, Jeanine J.; de Jong, Gerard; Vergeer, Jeannette M.; Hofman, Albert; Pols, Huibert A.P.; van Duijn, Cornelia M.

    2004-01-01

    Serum bilirubin is an important antioxidant that is found at increased levels in hereditary hemochromatosis patients. We hypothesized that increased levels of serum bilirubin may play a protective role against oxidative stress induced by iron overload in carriers of mutations in the hereditary hemoc

  11. Conjugated bilirubin in neonates with glucose-6-phosphate dehydrogenase deficiency.

    Science.gov (United States)

    Kaplan, M; Rubaltelli, F F; Hammerman, C; Vilei, M T; Leiter, C; Abramov, A; Muraca, M

    1996-05-01

    We used a system capable of measuring conjugated bilirubin and its monoconjugated and diconjugated fractions in serum to assess bilirubin conjugation in 29 glucose-6-phosphate dehydrogenase (G6PD)-deficient, term, male newborn infants and 35 control subjects; all had serum bilirubin levels > or = 256 mumol/L (15 mg/dI). The median value for diconjugated bilirubin was lower in the G6PD-deficient neonates than in control subjects (0.06 (range 0.00 to 1.84) vs 0.21 (range 0.00 to 1.02) mumol/L, p = 0.006). Diglucuronide was undetectable in 11 (38.9%) of the G6PD-deficient infants versus 3 (8.6%) of the control subjects (p = 0.015). These findings imply a partial defect of bilirubin conjugation not previously demonstrated in G6PD-deficient newborn infants.

  12. Trans-Cutaneous Bilirubinometery versus Serum Bilirubin in Neonatal Jaundice.

    Science.gov (United States)

    Mahram, Manoochehr; Oveisi, Sonia; Jaberi, Najmeh

    2015-12-01

    Hyperbilirubinemia is a common problem in neonates and causes serious complications. Thus, serial measurements of bilirubin should be done. This assessment is done through two methods of laboratory measurement in serum sample and transcutaneous bilirubinometer. This descriptive study compared transcutaneous bilirubin assessment and laboratory serum bilirubin. Bilirubin level was assessed among 256 neonates admitted to the Qods Children's Hospital in Qazvin- Iran, because of neonatal indirect jaundice, through two methods of transcutaneous bilirubinometery from two sites of forehead and sternum and laboratory measurement of bilirubin in serum. The cases were non-hemolytic icteric term neonates weighing 2500 gram or more and had not received phototherapy or other treatments. Neonates with hemolytic forms of jaundice, sepsis and suspicious to metabolic disorders were excluded. Assessments by means of KJ-8000 transcutaneous bilirubinometer from two sites of forehead and sternum and through laboratory measurement of serum bilirubin were registered and analyzed. The results of the current study showed that there was a correlation of 0.82 between serum bilirubin and transcutaneous forehead bilirubin assessment and for the used device sensitivity of 0.844; specificity of 0.842, Youden Index of 0.709 and Shortest of 0.042 for a cut-off of 12.4 in bilirubin of participants. Furthermore, Likelihood Ratio positive and negative (LR) were 5.665 and 0.164, respectively and diagnostic Odds Ratio (LR+/LR-) was 34.56. Transcutaneous bilirubinometery can be considered as a reliable tool to assess bilirubin for the screening of neonatal jaundice in term neonates.

  13. Relationship between serum bilirubin levels and optic neuritis

    Institute of Scientific and Technical Information of China (English)

    DENG Juan; LIANG Xue-mei; ZHANG Xiu-lan; LING Shi-qi; YANG Ting-ting; LI Min; PENG Fu-hua

    2013-01-01

    Background Bilirubin is the end product of heme catabolism and has strong antioxidant properties.Serum bilirubin levels are reported to be reduced in patients with multiple sclerosis (MS) and neuromyelitis optica (NMO).The pathophysiology of optic neuritis (ON) resembles that of MS; however,the role of endogenous bilirubin in ON is unclear.The aim of this study is to measure serum bilirubin levels in patients with ON,and to investigate the correlation between ON and serum antioxidant status of bilirubin.Methods Serum levels of bilirubin were measured in 42 patients with ON,50 patients with multiple sclerosis (MS),48 patients with neuromyelitis optica (NMO) and 48 healthy control subjects.Results Serum total bilirubin (Tbil),direct bilirubin (Dbil) and indirect bilirubin (Ibil) levels in patients with ON were significantly lower than those in the healthy controls.However,no statistical significance was found between levels in the ON and MS,ON and NMO,and MS and NMO groups.In patients with ON,serum Tbil,Dbil,and Ibil levels were lower in those with recurrence or those with ON for a longer duration (>1 year).Moreover,Tbil,Dbil,and Ibil concentrations were lower in patients with papillitis than in those with retrobulbar type ON,but the differences were not statistically significant.Conclusions Low antioxidant status may exist in patients with ON.But serum levels of Tbil,Dbil,and Ibil did not correlate with clinical presentations,such as recurrence,duration of disease and subtypes of ON.Low antioxidant status already existed in MS or NMO patients before systemic symptoms appeared.

  14. Anti-genotoxic potential of bilirubin in vivo

    DEFF Research Database (Denmark)

    Wallner, Marlies; Antl, Nadja; Rittmannsberger, Barbara;

    2013-01-01

    The bile pigment bilirubin is a known antioxidant and is associated with protection from cancer and cardiovascular disease (CVD) when present in too strong concentrations. Unconjugated bilirubin (UCB) might also possess anti-genotoxic potential by preventing oxidative damage to DNA. Moderately...... elevated bilirubin levels are found in individuals with Gilbert syndrome and more severe in the hyperbilirubinemic Gunn rat model. This study was therefore aimed to assess the levels of oxidative damage to DNA in Gilbert syndrome subjects and Gunn rats compared to matched controls. Seventy-six individuals...

  15. Transcutaneous bilirubin nomograms in African neonates

    Science.gov (United States)

    Mabogunje, Cecilia A.; Imosemi, Donald O.; Emokpae, Abieyuwa A.

    2017-01-01

    Background The use of transcutaneous bilirubin (TcB) as a screening tool, based on relevant population-specific nomogram, or proxy for total serum bilirubin (TSB) levels in assessing the risk of subsequent hyperbilirubinemia is supported by several clinical guidelines on the management of neonatal hyperbilirubinemia. However, while TcB has been found to significantly over-estimate TSB in neonates of African-American ancestry, with variations across TcB devices, no nomogram has been specifically reported for this racial group. This study therefore set out to develop TcB nomograms for healthy late pre-term and term black African neonates derived from two widely used bilirubinometers. Methods A retrospective analysis of 12,377 TcB measurements obtained from 6,373 neonates in the first postnatal week, over a period of 48 months using Bilichek and JM-103 bilirubinometers. TcB percentiles were computed from hour-specific TcB values and nomograms developed for each of the screening devices. Predictive ability of the 75th and 95th percentiles to detect significant hyperbilirubinemia was evaluated between 24–96 hours of age. The 95th percentile curve was compared with those from other populations. Results The velocity of TcB rise at 75th and 95th percentiles was generally higher with JM-103 than Bilichek. Both percentiles also peaked at higher TcB levels with JM-103. The 95th percentile for both instruments showed a downward trend as from approximately 114 hours. Both instruments had high negative predictive values across the selected time-epochs and lower discriminatory ability than reported in non-black populations. Conclusions The predictive utility of TcB as a potential screening tool varies across devices in black African neonates with or without risk of significant hyperbilirubinemia, and lower than levels reported in non-black populations. Equipment-specific nomograms should be considered for TcB monitoring in this racial population where TSB is not routinely

  16. The clinical syndrome of bilirubin-induced neurologic dysfunction.

    Science.gov (United States)

    Bhutani, Vinod K; Johnson-Hamerman, Lois

    2015-02-01

    Clinicians have hypothesized a spectrum of minor neurologic manifestations, consistent with neuroanatomical reports and collectively termed as a "syndrome of bilirubin-induced neurologic dysfunction (BIND)," which can occur in the absence of classical kernicterus. The current review builds on these initial reports with a focus on clinical signs and symptoms that are assessed by standardized tools and manifest from neonatal age to childhood. These clinical manifestations are characterized by the following domains: (i) neuromotor signs; (ii) muscle tone abnormalities; (iii) hyperexcitable neonatal reflexes; (iv) variety of neurobehavior manifestations; (v) speech and language abnormalities; and (vi) evolving array of central processing abnormalities, such as sensorineural audiology and visuomotor dysfunctions. Concerns remain that the most vulnerable infants are likely to acquire BIND, either because their exposure to bilirubin is not identified as severe enough to need treatment or is prolonged but slightly below current threshold levels for intervention. Knowing that a total serum/plasma bilirubin (TB) level is not the most precise indicator of neurotoxicity, the role of expanded biomarkers or a "bilirubin panel" has yet to be validated in prospective studies. Future studies that correlate early "toxic" bilirubin exposure to long-term academic potential of children are needed to explore new insights into bilirubin's effect on the structural and functional maturation of an infant's neural network topology.

  17. Ibuprofen augments bilirubin toxicity in rat cortical neuronal culture.

    Science.gov (United States)

    Berns, Monika; Toennessen, Margit; Koehne, Petra; Altmann, Rodica; Obladen, Michael

    2009-04-01

    Premature infants are at risk for bilirubin-associated brain damage. In cell cultures bilirubin causes neuronal apoptosis and necrosis. Ibuprofen is used to close the ductus arteriosus, and is often given when hyperbilirubinemia is at its maximum. Ibuprofen is known to interfere with bilirubin-albumin binding. We hypothesized that bilirubin toxicity to cultured rat embryonic cortical neurons is augmented by coincubation with ibuprofen. Incubation with ibuprofen above a concentration of 125 microg/mL reduced cell viability, measured by methylthiazole tetrazolium reduction, to 68% of controls (p < 0.05). Lactate dehydrogenase (LDH) release increased from 29 to 38% (p < 0.01). The vehicle solution did not affect cell viability. Coincubation with 10 microM unconjugated bilirubin (UCB)/human serum albumin in a molar ratio of 3:1 and 250 microg/mL ibuprofen caused additional loss of cell viability and increased LDH release (p < 0.01), DNA fragmentation, and activated caspase-3. Preincubation with the pan-caspase inhibitor z-val-ala-asp-fluoromethyl ketone abolished ibuprofen- and UCB-induced DNA fragmentation. The study demonstrates that bilirubin in low concentration of 10 microM reduces neuron viability and ibuprofen increases this effect. Apoptosis is the underlying cell death mechanism.

  18. Relationship between bilirubin free radical and formation of pigment gallstone

    Institute of Scientific and Technical Information of China (English)

    Xiang-Tao Liu; Jian Hu

    2002-01-01

    In this paper, we summarize the main progresses made inour group in the field of the mechanism of pigment gallstoneformation. It was found that after treetment with freeradicals, bilirubin (BR) was changed into free radical itself,and a semiquinone free radical and a superoxide free radicalbound with metal were recognized, which was detected byESR (electron spin resonance). By the meana of NMR(nuclear magnetic resonance) and IR (Infra-red spectra), itwas postulated that bilirubin polymerized through thereaction between the vinyl group and the hydroxyl groupunder the attack of free radicals. It was also found thatbilirubin free radical were liable to calcify in a kinetic study.Because of its chemical properties, bilirubin free radical wasshown to be cytotoxic to hepetocyte, which wasdemonstrated based on the following facts: induction ofphospholipid peroxidation (LPO), leakage of lactatedehydrogenase (LDH) and decrease of glutathione. As tothe mechanism of bilirubin-induced cytotoxicity, it waspostulated that the main target of bilirubin free radical wasthe cell membrane, including phospholipid and membranebound proteins, especially spectrin, a content ofcytoskeleton. Based on the results mentioned above, it wasdeduced that bilirubin free radical is the key factor thatinitiates and promotes the formation of pigment gallstone,which is consistent with other researches in recent years.

  19. Blood-brain interfaces and bilirubin-induced neurological diseases.

    Science.gov (United States)

    Ghersi-Egea, J F; Gazzin, S; Strazielle, N

    2009-01-01

    The endothelium of the brain microvessels and the choroid plexus epithelium form highly specialized cellular barriers referred to as blood-brain interfaces through which molecular exchanges take place between the blood and the neuropil or the cerebrospinal fluid, respectively. Within the brain, the ependyma and the pia-glia limitans modulate exchanges between the neuropil and the cerebrospinal fluid. All these interfaces are key elements of neuroprotection and fulfill trophic functions; both properties are critical to harmonious brain development and maturation. By analogy to hepatic bilirubin detoxification pathways, we review the transport and metabolic mechanisms which in all these interfaces may participate in the regulation of bilirubin cerebral bioavailability in physiologic conditions, both in adult and in developing brain. We specifically address the role of ABC and OATP transporters, glutathione-S-transferases, and the potential involvement of glucuronoconjugation and oxidative metabolic pathways. Regulatory mechanisms are explored which are involved in the induction of these pathways and represent potential pharmacological targets to prevent bilirubin accumulation into the brain. We then review the possible alteration of the neuroprotective and trophic barrier functions in the course of bilirubin-induced neurological dysfunctions resulting from hyperbilirubinemia. Finally, we highlight the role of the blood-brain and blood-CSF barriers in regulating the brain biodisposition of candidate drugs for the treatment or prevention of bilirubin-induced brain injury.

  20. Functional polyethersulfone particles for the removal of bilirubin.

    Science.gov (United States)

    Jiang, Xin; Xiang, Tao; Xie, Yi; Wang, Rui; Zhao, Weifeng; Sun, Shudong; Zhao, Chang-Sheng

    2016-02-01

    In this study, polyethersulfone/poly (glycidyl methacrylate) particles are prepared via in situ cross-linked polymerization coupled with a phase inversion technique. The surfaces of these particles are then further modified by grafting amino groups using tetraethylenepentamine, dethylenetriamine, ethylenediamine, or 1,6-hexanediamine for the removal of bilirubin. The particles are characterized by Flourier transform infrared spectroscopy, thermogravimetric analysis, and scanning electron microscopy. Batch adsorption experiments are performed to verify the adsorption capability, and the effect of bilirubin initial concentration, bovine serum albumin concentration, and solution ionic strength on the adsorption is also investigated. In addition, both adsorption kinetic and isotherm models are applied to analyze the adsorption process of bilirubin, and a particle column is used to further study the bilirubin removal ability.To prove that the method was a universal portal to prepare functional particles, polysulfone, polystyrene, and poly(vinylidene fluoride) based functional particles were also prepared and used for the removal of bilirubin. This study and the results indicated that the particles had a great potential to be used in hemoperfusion treatment for hyperbilirubinemia.

  1. Study on Removal of Bilirubin with Magnetic Affinity Separation Technique

    Institute of Scientific and Technical Information of China (English)

    张凤宝; 王淑兰; 徐辉; 张国亮

    2003-01-01

    An affinity adsorbent, Cibacron Blue 3GA immobilized magnetic polyvinyl alcohol (PVA) microspheres was used for bilirubin removal taking the advantage of easy separation of magnetic sorbent from the biosystem.Fe3 O4 superparamagnetic particles was synthesized with hydrothermal reaction of ferrous chloride (FeC12) and ferric chloride (FeCl3). Such magnetic particles are then encapsulated in biocompatible PVA to form magnetic polymer microspheres sized from 2 to 15 nm with hydroxyl groups on its surface. Cibacron Blue 3GA, a dye-ligand, was covalently coupled with the polyvinyl alcohol through the nucleophilic reaction between the chloride of its triazine ring and the hydroxyl groups of PVA molecules under alkaline condition. The affinity adsorbent carried 21.1μmol Cibacron Blue 3GA per gram magnetic polymer microspheres was used to remove unconjugated and conjugated bilirubin from the solution which was composed of bilirubin or bilirubin and protein. After the adsorption, the adsorbent loaded with bilirubin was removed easily in the magnetic field.

  2. Influence of hemoglobin on non-invasive optical bilirubin sensing

    Science.gov (United States)

    Jiang, Jingying; Gong, Qiliang; Zou, Da; Xu, Kexin

    2012-03-01

    Since the abnormal metabolism of bilirubin could lead to diseases in the human body, especially the jaundice which is harmful to neonates. Traditional invasive measurements are difficult to be accepted by people because of pain and infection. Therefore, the real-time and non-invasive measurement of bilirubin is of great significance. However, the accuracy of currently transcutaneous bilirubinometry(TcB) is generally not high enough, and affected by many factors in the human skin, mostly by hemoglobin. In this talk, absorption spectra of hemoglobin and bilirubin have been collected and analyzed, then the Partial Least Squares (PLS) models have been built. By analyzing and comparing the Correlation and Root Mean Square Error of Prediction(RMSEP), the results show that the Correlation of bilirubin solution model is larger than that of the mixture solution added with hemoglobin, and its RMSEP value is smaller than that of mixture solution. Therefore, hemoglobin has influences on the non-invasive optical bilirubin sensing. In next step, it is necessary to investigate how to eliminate the influence.

  3. Effect of pH and temperature on the binding of bilirubin to human erythrocyte membranes

    Indian Academy of Sciences (India)

    H Rashid; Mohammad K Ali; S Tayyab

    2000-06-01

    Effect of pH and temperature on the binding of bilirubin to human erythrocyte membranes was studied by incubating the membranes at different pH and temperatures and determining the bound bilirubin. At all pH values, the amount of membrane-bound bilirubin increased with the increase in bilirubin-to-albumin molar ratios (B/As), being highest at lower pH values in all cases. Further, linear increase in bound bilirubin with the increase in bilirubin concentration in the incubate was observed at a constant B/A and at all pH values. However, the slope value increased with the decrease in pH suggesting more bilirubin binding to membranes at lower pH values. Increase in bilirubin binding at lower pH can be explained on the basis of increased free bilirubin concentration as well as more conversion of bilirubin dianion to monoanion. Temperature dependence of bilirubin binding to membranes was observed within the temperature range of 7°–60°C, showing minimum binding at 27°C and 37°C which increased on either side. Increase in bilirubin binding at temperatures lower than 20°C and higher than 40°C can be ascribed to the change in membrane topography as well as bilirubin-albumin interaction.

  4. Studies on the molecular significance in the interaction of bilirubin with collagen.

    Science.gov (United States)

    Nagarajan, Usharani; Gladstone Christopher, Jayakumar; Chandrasekaran, Bangaru; Jonnalagadda, Raghava Rao; Balachandran, Unni Nair; Kohsaku, Kawakami

    2013-10-01

    The present investigation is aimed to understand the physiological significance of bilirubin interaction with collagen. In human skin, collagen absorbs both free bilirubin and serum bound bilirubin from the human system. Interaction between bilirubin and collagen depends on time, temperature and concentration of bilirubin. There is an increase in the aggregation rate of collagen in the presence of biliruibin. At physiological condition, 125 nM of bilirubin is the maximum concentration absorbed by per mg of collagen molecule. Bilirubin accelerates the lateral growth of collagen fibrils by shifting its rate of nucleation. Moreover, collagen-bilirubin complex exhibit a tendency to undergo adsorption onto the surface of the fibroblast cells, showing detrimental effects on fibroblasts proliferations. Based on the collagen binding assays, the binding of bilirubin to collagen is found to be electrostatic in nature, which confirms binding between the amino acid fragment of α1 (I) region of collagen and carboxyl group of bilirubin. The biotinylated bilirubin derivatives show better binding to α1 (I) chain rather than α2 (I) chains which clearly designates that bilirubin shows greater affinity to α1 chains of collagen. This novel approach directs to reduce the occurrence of bilirubin in hyperbilirubinemia patients.

  5. Measurements of neonatal bilirubin and albumin concentrations: a need for improvement and quality control

    NARCIS (Netherlands)

    Imhoff, D.E. van; Dijk, P.H.; Weykamp, C.W.; Cobbaert, C.M.; Hulzebos, C.V.; Liem, K.D.

    2011-01-01

    Accurate and precise bilirubin and albumin measurements are essential for proper management of jaundiced neonates. Data hereon are lacking for Dutch laboratories. We aimed to determine variability of measurements of bilirubin and albumin concentrations typical for (preterm) neonates. Aqueous, human

  6. Measurements of neonatal bilirubin and albumin concentrations : a need for improvement and quality control

    NARCIS (Netherlands)

    van Imhoff, Deirdre E.; Dijk, Peter H.; Weykamp, Cas W.; Cobbaert, Christa M.; Hulzebos, Christian V.

    2011-01-01

    Accurate and precise bilirubin and albumin measurements are essential for proper management of jaundiced neonates. Data hereon are lacking for Dutch laboratories. We aimed to determine variability of measurements of bilirubin and albumin concentrations typical for (preterm) neonates. Aqueous, human

  7. DECREASED BILIRUBIN TRANSPORT IN THE PERFUSED LIVER OF ENDOTOXEMIC RATS

    NARCIS (Netherlands)

    ROELOFSEN, H; VANDERVEERE, CN; OTTENHOFF, R; SCHOEMAKER, B; JANSEN, PLM; ELFERINK, RPJO

    1994-01-01

    Background/Aims: Hyperbilirubinemia associated with sepsis is frequently observed in humans. In this study, an experimental rat model was developed to study bilirubin metabolism and transport during endotoxemia. Methods: Rats were injected intravenously with a single bolus of lipopolysaccharide (1 m

  8. Plasma bilirubin and late graft failure in renal transplant recipients

    NARCIS (Netherlands)

    Deetman, Petronella E.; Zelle, Dorien M.; van der Heide, Jaap J. Homan; Navis, Gerjan J.; Gans, Reinold O. B.; Bakker, Stephan J. L.

    2012-01-01

    Exogenous bilirubin has been shown to protect against oxidative stress in ischemia-reperfusion injury. Oxidative stress has been implicated in the pathophysiology of chronic transplant dysfunction leading to late graft failure after renal transplantation. We prospectively investigated whether high e

  9. Nanofibrous polymeric beads from aramid fibers for efficient bilirubin removal.

    Science.gov (United States)

    Peng, Zihang; Yang, Ye; Luo, Jiyue; Nie, Chuanxiong; Ma, Lang; Cheng, Chong; Zhao, Changsheng

    2016-08-16

    Polymer based hemoperfusion has been developed as an effective therapy to remove the extra bilirubin from patients. However, the currently applied materials suffer from either low removal efficiency or poor blood compatibility. In this study, we report the development of a new class of nanofibrous absorbent that exhibited high bilirubin removal efficiency and good blood compatibility. The Kevlar nanofiber was prepared by dissolving micron-sized Kevlar fiber in proper solvent, and the beads were prepared by dropping Kevlar nanofiber solutions into ethanol. Owing to the nanofiborous structure of the Kevlar nanofiber, the beads displayed porous structures and large specific areas, which would facilitate the adsorption of toxins. In the adsorption test, it was noticed that the beads possessed an adsorption capacity higher than 40 mg g(-1) towards bilirubin. In plasma mimetic solutions, the beads still showed high bilirubin removal efficiency. Furthermore, after incorporating with carbon nanotubes, the beads were found to have increased adsorption capacity for human degradation waste. Moreover, the beads showed excellent blood compatibility in terms of a low hemolysis ratio, prolonged clotting times, suppressed coagulant activation, limited platelet activation, and inhibited blood related inflammatory activation. Additionally, the beads showed good compatibility with endothelial cells. In general, the Kevlar nanofiber beads, which integrated with high adsorption capacity, good blood compatibility and low cytotoxicity, may have great potential for hemoperfusion and some other applications in biomedical fields.

  10. Serum bilirubin levels, polymorphisms and risk for coronary artery disease

    OpenAIRE

    Lingenhel, Arno; Kollerits, Barbara; Johannes P. Schwaiger; Hunt, Steven C.; Gress, Richard; Hopkins, Paul N.; Schoenborn, Veit; Heid, Iris M; Kronenberg, Florian

    2008-01-01

    Serum bilirubin levels, UGT1A1 polymorphisms and risk for coronary artery disease correspondence: Corresponding author. Tel.: +43 512 9003 70560; fax: +43 512 9003 73560. (Kronenberg, Florian) (Kronenberg, Florian) Division of Genetic Epidemiology; Department of Medical Genetics, Molecular and Clinical Pharmacology; Innsbruck Medical University - AUSTRIA (Lingenhel, Arno) Division of Genetic Epidemiology; Depa...

  11. RAPID ASSOCIATION OF UNCONJUGATED BILIRUBIN WITH AMORPHOUS CALCIUM-PHOSPHATE

    NARCIS (Netherlands)

    VANDERVEERE, CN; SHOEMAKER, B; VANDERMEER, R; GROEN, AK; JANSEN, PLM; ELFERINK, RPJO

    1995-01-01

    The association of unconjugated bilirubin (UCB) with amorphous calcium phosphate was studied in vitro. To this end UCB, solubilized in different micellar bile salt solutions, was incubated with freshly prepared calcium phosphate precipitate. It was demonstrated that amorphous calcium phosphate (ACP)

  12. Conformational changes in the bilirubin-human serum albumin complex at extreme alkaline pH

    DEFF Research Database (Denmark)

    Honoré, B; Frandsen, P C

    1986-01-01

    Light-absorption, c.d. and fluorescence of the bilirubin-albumin complex were investigated at extreme alkaline pH. Above pH 11.1 albumin binds the bilirubin molecule, twisted oppositely to the configuration at more neutral pH. On the basis of light-absorption it is shown that two alkaline...... between tryptophan-214 and bilirubin, and partly exposing the liganded bilirubin to the solvent. Udgivelsesdato: 1986-Jun-1...

  13. Clinical Significance of Serum Bilirubin Detection of Patient with Coronary Heart Disease

    Institute of Scientific and Technical Information of China (English)

    TAO Li; LUO Rui; ZHUANG Diankui

    2004-01-01

    Objective To explore the relation between serum bilirubin and coronary heart disease.Methods Compare the level of serum bilirubin among patients with coronary heart disease, patients with other disease and normal persons. Results The level of serum bilirubin of patients with coronary heart disease is higher than that of normal persons. Conclusion The reduction of density of serum bilirubin is one of the independent risk factors of coronary heart disease.

  14. Interaction of bilirubin with Ag and Au ions: green synthesis of bilirubin-stabilized nanoparticles

    Science.gov (United States)

    Shukla, Shashi P.; Roy, Mainak; Mukherjee, Poulomi; Tyagi, A. K.; Mukherjee, Tulsi; Adhikari, Soumyakanti

    2012-07-01

    We report a simple green chemistry to synthesize and stabilize monodispersed silver and gold nanoparticles sols by reducing aqueous solution of the respective metal salts in the presence of bilirubin (BR). No additional capping agent was used in the process of stabilization of the nanoparticles. As a completely new finding, we have observed that BR known to be toxic at higher concentration in one hand and conversely an antioxidant at physiological concentration reduces these metal ions to form the respective metal nanoparticles. Moreover, BR and its oxidized products also serve as capping agents to the nanoparticles. The particles were characterized by transmission electron microscopy. BR and its oxidized products capped nanoparticles are stable for months. The UV-Vis absorption spectra of the silver sol show the plasmon peak of symmetric spherical particles which was further reflected in the TEM images. The sizes of the silver particles were about 5 nm. These silver particles showed reasonably high antibacterial activity in Gram negative wild type E. coli. In the case of interaction of BR with gold ions, we could obtain cubic gold nanoparticles of average sizes 20-25 nm. Possible modes of anchorage of BR and/its oxidized products to silver nanoparticles were demonstrated by surface-enhanced resonance Raman spectroscopy (SERS) that in turn demonstrated the feasibility of using these nanoparticles as SERS substrates.

  15. Interaction of bilirubin with Ag and Au ions: green synthesis of bilirubin-stabilized nanoparticles

    Energy Technology Data Exchange (ETDEWEB)

    Shukla, Shashi P. [Bhabha Atomic Research Centre, Radiation and Photochemistry Division (India); Roy, Mainak [Bhabha Atomic Research Centre, Chemistry Division (India); Mukherjee, Poulomi [Bhabha Atomic Research Centre, Nuclear Agriculture and Biotechnology Division (India); Tyagi, A. K. [Bhabha Atomic Research Centre, Chemistry Division (India); Mukherjee, Tulsi [Bhabha Atomic Research Centre, Chemistry Group (India); Adhikari, Soumyakanti, E-mail: asoumya@barc.gov.in [Bhabha Atomic Research Centre, Radiation and Photochemistry Division (India)

    2012-07-15

    We report a simple green chemistry to synthesize and stabilize monodispersed silver and gold nanoparticles sols by reducing aqueous solution of the respective metal salts in the presence of bilirubin (BR). No additional capping agent was used in the process of stabilization of the nanoparticles. As a completely new finding, we have observed that BR known to be toxic at higher concentration in one hand and conversely an antioxidant at physiological concentration reduces these metal ions to form the respective metal nanoparticles. Moreover, BR and its oxidized products also serve as capping agents to the nanoparticles. The particles were characterized by transmission electron microscopy. BR and its oxidized products capped nanoparticles are stable for months. The UV-Vis absorption spectra of the silver sol show the plasmon peak of symmetric spherical particles which was further reflected in the TEM images. The sizes of the silver particles were about 5 nm. These silver particles showed reasonably high antibacterial activity in Gram negative wild type E. coli. In the case of interaction of BR with gold ions, we could obtain cubic gold nanoparticles of average sizes 20-25 nm. Possible modes of anchorage of BR and/its oxidized products to silver nanoparticles were demonstrated by surface-enhanced resonance Raman spectroscopy (SERS) that in turn demonstrated the feasibility of using these nanoparticles as SERS substrates.

  16. Intestinal excretion of unconjugated bilirubin in man and rats with inherited unconjugated hyperbilirubinemia

    NARCIS (Netherlands)

    Kotal, P; VanderVeere, CN; Sinaasappel, M; Elferink, RO; Vitek, L; Brodanova, M; Jansen, PLM; Fevery, J

    1997-01-01

    Patients with Crigler-Najjar syndrome and Gunn rats cannot form bilirubin glucuronides owing to a lack of bilirubin UDP-glucuronosyltransferase activity. Because increased serum and tissue bilirubin levels remain constant, an alternative excretory route has to substitute for this deficiency. Gunn ra

  17. Distant Determination of Bilirubin Distribution in Skin by Multi-Spectral Imaging

    Science.gov (United States)

    Saknite, I.; Jakovels, D.; Spigulis, J.

    2011-01-01

    For mapping the bilirubin distribution in bruised skin the multi-spectral imaging technique was employed, which made it possible to observe temporal changes of the bilirubin content in skin photo-types II and III. The obtained results confirm the clinical potential of this technique for skin bilirubin diagnostics.

  18. Bilirubin binding with liver cystatin induced structural and functional changes.

    Science.gov (United States)

    Mustafa, Mir Faisal; Bano, Bilqees

    2014-05-01

    Cysteine proteinases and their inhibitors play a significant role in the proteolytic environment of the cells. Inhibitors of cysteine proteinases regulate the activity of these enzymes helping in checking the degdration activity of cathepsins. The bilirubin secreated by liver cells can bind to cystatin present in the liver resulting in its functional inactivation, which may further lead to the increase in cathepsins level causing liver cirrhosis. In case of some pathophysiological conditions excess bilirubin gets accumulated e.g. in presence of Fasciola hepatica (liver fluke) in mammals and humans, leading to liver cirrhosis and possibly jaundice or normal blockade of bile duct causing increased level of bilirubin in blood. Protease-cystatin imbalance causes disease progression. In the present study, Bilirubin (BR) and liver cystatin interaction was studied to explore the cystatin inactivation and structural alteration. The binding interaction was studied by UV-absorption, FT-IR and fluorescence spectroscopy. The quenching of protein fluorescence confirmed the binding of BR with buffalo liver cystatin (BLC). Stern-Volmer analysis of BR-BLC system indicates the presence of static component in the quenching mechanism and the number of binding sites to be close to 1. The fluorescence data proved that the fluorescence quenching of liver cystatin by BR was the result of BR-cystatin complex formation. FTIR analysis of BR-Cystatin complex revealed change in the secondary structure due to perturbation in the microenvironment further confirmed by the decreased caseinolytic activity of BLC against papain. Fluorescence measurements also revealed quenching of fluorescence and shift in peak at different time intervals and at varying pH values. Photo-illumination of BR-cystatin complex causes change in the surrounding environment of liver cystatin as indicated by red-shift. The binding constant for BR-BLC complex was found to be 9.279 × 10(4) M(-1). The cystatin binding with

  19. Bilirubin exerts pro-angiogenic property through Akt-eNOS-dependent pathway.

    Science.gov (United States)

    Ikeda, Yasumasa; Hamano, Hirofumi; Satoh, Akiho; Horinouchi, Yuya; Izawa-Ishizawa, Yuki; Kihira, Yoshitaka; Ishizawa, Keisuke; Aihara, Ken-Ichi; Tsuchiya, Koichiro; Tamaki, Toshiaki

    2015-11-01

    Low serum bilirubin levels are associated with the risk of cardiovascular diseases including peripheral artery disease. Bilirubin is known to exert its property such as antioxidant effect or the enhancement of flow-mediated vasodilation, however, bilirubin action on angiogenesis remains unclear. To investigate the molecular mechanism of bilirubin on angiogenic effect, we first employed C57BL/6J mice with unilateral hindlimb ischemia surgery and divided the mice into two groups (vehicle-treated group and bilirubin-treated group). The analysis of laser speckle blood flow demonstrated the enhancement of blood flow recovery in response to ischemia of mice with bilirubin treatment. The density of capillaries was significantly higher in ischemic-adductor muscles of bilirubin-treated mice. The phosphorylated levels of endothelial nitric oxide synthase (eNOS) and Akt were increased in ischemic skeletal muscles of mice with bilirubin treatment compared with vehicle treatment. In in vitro experiments by using human aortic endothelial cells, bilirubin augmented eNOS and Akt phosphorylation, cell proliferation, cell migration and tube formation. These bilirubin actions on endothelial cell activation were inhibited by LY294002, a phosphatidylinositol 3-kinase inhibitor. In conclusion, bilirubin promotes angiogenesis through endothelial cells activation via Akt-eNOS-dependent manner.

  20. Determination of bilirubin by thermal lens spectrometry and studies of its transport into hepatic cells

    Science.gov (United States)

    Margon, A.; Terdoslavich, M.; Cocolo, A.; Decorti, G.; Passamonti, S.; Franko, M.

    2005-06-01

    The liver is responsible for clearance of bilirubin, the end product of heme catabolism, from the bloodstream. The main aim of our investigation was to determine the role of the carrier protein bilitranslocase in bilirubin uptake into the liver. Our experiments consisted of exposing cell cultures to bilirubin solutions under different conditions and measuring the uptake of bilirubin into the cells. However, since bilirubin is only slightly soluble in aqueous solution (pH 7.4), we had to use bilirubin concentrations that are far below the limit of detection of the commonly used techniques (e.g. LOD for HPLC with UV-Vis detection \\cong 10 μM). TLS showed up to be a suitable technique for investigation of bilirubin uptake with an LOD of 2 nM. Under basal conditions, bilirubin uptake did not occur. However, increase of cytosolic NADH due to catabolism of specific substrates (e.g. lactate or ethanol) seemed to trigger bilirubin uptake. Furthermore, bilirubin uptake was completely inhibited by addition of specific anti-bilitranslocase antibodies. We can thus infer that, under these conditions, bilitranslocase is the main bilirubin transporter.

  1. Photodamage of the cells in culture sensitized with bilirubin

    Science.gov (United States)

    Kozlenkova, O. A.; Plavskaya, L. G.; Mikulich, A. V.; Leusenko, I. A.; Tretyakova, A. I.; Plavskii, V. Yu

    2016-08-01

    It has been shown that exposure to radiation of LED sources of light with an emission band maximum at about 465 and 520 nm having substantially identical damaging effects on animal cells in culture, that are in a logarithmic growth phase and preincubated with pigment. Photobiological effect is caused by photodynamic processes involving singlet oxygen generated by triplet excited sensitizer. Mono-exponential type dependence of cell survival on the energy dose indicates that it is bilirubin that acts as a sensitizer but not its photoproducts. The inclusion of bilirubin in the cells, where it is primarily localized in the mitochondria cells, it is accompanied by multiple amplification photochemical stability compared to pigment molecules bound with albumin

  2. A New Bilirubin Concentration Detection Method by Light Reflection

    CERN Document Server

    Subasilar, B

    1999-01-01

    A new and simple method of blood bilirubin detection through light reflection from skin is developed. The basic improvement over the existing methods is in the design of the light emitter and detector geometry which facilitates a two-stream plane parallel homogenous medium solution to the emitting-scatterig radiative transfer equation. The forward peak in the scattering phase function that is characteristic of water droplets and water filled media is accounted for through a proper method named delta-Eddington approximation.

  3. The Relationship of the Anti-Oxidant Bilirubin with Free Thyroxine Is Modified by Insulin Resistance in Euthyroid Subjects

    NARCIS (Netherlands)

    Deetman, Petronella E.; Bakker, Stephan J. L.; Kwakernaak, Arjan J.; Navis, Gerjan; Dullaart, Robin P. F.

    2014-01-01

    Background: The strong anti-oxidative properties of bilirubin largely explain its cardioprotective effects. Insulin resistance is featured by low circulating bilirubin. Thyroid hormone affects both bilirubin generation and its biliary transport, but it is unknown whether circulating bilirubin is ass

  4. Bilirubin: an endogenous molecule with antiviral activity in vitro.

    Directory of Open Access Journals (Sweden)

    Rosaria eSantangelo

    2012-03-01

    Full Text Available Bilirubin-IX-alpha (BR is the final product of heme metabolism through the heme oxygenase/biliverdin reductase (HO/BVR system. Previous papers reported on the microbicidal effects of the HO by-products biliverdin-IX-alpha, carbon monoxide and iron, through either direct or indirect mechanisms. In this paper the evidence of a virucidal effect of BR against human herpes simplex virus type 1 (HSV-1 and the enterovirus EV71 was provided. Bilirubin-IX-alpha, at concentrations 1-10 µM, close to those found in blood and tissues, significantly reduced HSV-1 and EV71 replication in Hep-2 and Vero cell lines, respectively. Bilirubin-IX-alpha inhibited viral infection of Hep-2 and Vero cells when given 2 hours before, concomitantly and 2 hours after viral infection. Furthermore, BR retained its antiviral activity even complexed with a saturating concentration of human serum-albumin. Moreover, 10 µM BR increased the formation of nitric oxide and the phosphorylation of JNK in Vero and Hep-2 cell lines, respectively, thus implying a role of these two pathways in the mechanism of antiviral activity of the bile pigment. In conclusion, these results support the antiviral effect of BR against HSV-1 and enterovirus in vitro, and put the basis for further basic and clinical studies to understand the real role of BR as an endogenous antiviral molecule.

  5. Molecularly Imprinted Quartz Crystal Microbalance Sensor (QCM for Bilirubin Detection

    Directory of Open Access Journals (Sweden)

    Çiğdem Çiçek

    2016-11-01

    Full Text Available This study aims the preparation of a QCM sensor for the detection of bilirubin in human plasma. Bilirubin-imprinted poly-(2-hydroxyethyl methacrylate-N-methacryloyl-l-tryptophan methyl ester (PHEMATrp nanofilm (MIP on the gold surface of a QCM chip was synthesized by the molecular imprinting technique. Meanwhile, the non-imprinted PHEMATrp (NIP nanofilm was synthesized by the same experimental technique to examine the imprinting effect. Characterization of MIP and NIP nanofilms on the QCM chip surface was achieved by atomic force microscopy (AFM, ellipsometry, Fourier transform infrared spectrophotometry-attenuated total reflectance (FTIR-ATR and contact angle measurements (CA. The observations indicated that the nanofilm was almost in a monolayer. Thereinafter, the imprinted and the non-imprinted QCM chips were connected to the QCM system to investigate kinetic and affinity properties. In order to examine the selectivity of the MIP-PHEMATrp nanofilm, competitive adsorption of bilirubin with cholesterol and estradiol was performed. Limit of detection (LOD and limit of quantitation (LOQ values were calculated as 0.45 μg/mL and 0.9 μg/mL, respectively.

  6. Spectroscopic studies on the interaction of bilirubin with liver cystatin.

    Science.gov (United States)

    Shah, Aaliya; Bano, Bilqees

    2011-02-01

    Studies on the role of endogenous metabolites such as bilirubin and their interactions with biomolecules have attracted considerable attention over the past several years. In this work, the interaction of bilirubin (BR) with purified goat liver cystatin (LC) was studied using fluorescence and ultraviolet (UV) spectroscopy. The fluorescence data proved that the fluorescence quenching of liver cystatin by BR was the result of BR-cystatin complex formation. Stern-Volmer analysis of fluorescence quenching data showed the binding constant to be 9.27 x 10⁴ M⁻¹ and the number of binding sites to be close to unity. The conformation of the BR-cystatin complex was found to change upon varying the pH of the complex. The BR-cystatin complex was found to have reduced papain inhibitory activity. Photo-illumination of BR-cystatin complex causes perturbation in the micro-environment of goat liver cystatin as indicated by red-shift. This report summarizes our research efforts to reveal the mechanism of interaction of bilirubin with liver cystatin.

  7. Association between flavonoid-rich fruit and vegetable consumption and total serum bilirubin.

    Science.gov (United States)

    Loprinzi, Paul D; Mahoney, Sara E

    2015-03-01

    Emerging work demonstrates that serum bilirubin is a novel biomarker implicated in cardiovascular and metabolic diseases. However, we have a limited understanding of the influence of flavonoid-rich fruit and vegetable consumption on bilirubin levels, which was the purpose of this study. Data from the 2003 to 2006 National Health and Nutrition Examination survey were used (n = 1783; 18-85 years of age), with analyses performed in 2014. Total serum bilirubin was measured from a blood sample. Using a food frequency questionnaire (FFQ), a flavonoid index variable was created summing the frequency of consumption of flavonoid-rich foods. After adjustments, greater consumption of flavonoid-rich fruits and vegetables was positively associated with bilirubin levels. Our findings suggest an association between flavonoid-rich fruit and vegetable consumption and bilirubin levels. If confirmed by prospective and experimental studies, then regular consumption of flavonoid-rich fruits and vegetables should be promoted to increase levels of bilirubin.

  8. High preoperative bilirubin values protect against reperfusion injury after live donor liver transplantation.

    Science.gov (United States)

    Spetzler, Vinzent N; Goldaracena, Nicolas; Kaths, Johann M; Marquez, Max; Selzner, Nazia; Cattral, Mark S; Greig, Paul D; Lilly, Les; McGilvray, Ian D; Levy, Gary A; Ghanekar, Anand; Renner, Eberhard L; Grant, David R; Selzner, Markus

    2015-11-01

    Heme Oxygenase-1 and its product biliverdin/bilirubin have been demonstrated to protect against ischemia/reperfusion injury (IRI). We investigated whether increased preoperative bilirubin values of transplant recipients decrease IRI. Preoperative bilirubin levels of live donor liver recipients were correlated to postoperative liver transaminase as a marker of IRI. Additionally, two recipient groups with pretransplant bilirubin levels >24 μmol/l (n = 348) and ≤24 μmol/l (n = 118) were compared. Post-transplant liver function, complications, length of hospital stay, and patient and graft survival were assessed. Preoperative bilirubin levels were negatively correlated to the postoperative increase in transaminases suggesting a protective effect against IRI. The maximal rise of ALT after transplantation in high versus low bilirubin patients was 288 (-210-2457) U/l vs. 375 (-11-2102) U/l, P = 0.006. Bilirubin remained a significant determining factor in a multivariate linear regression analysis. The MELD score and its individual components as a marker of severity of chronic liver disease were significantly higher in the high versus low bilirubin group (P bilirubin levels of liver recipients before live donor transplantation is associated with decreased postoperative IRI.

  9. Bilirubin Increases Insulin Sensitivity by Regulating Cholesterol Metabolism, Adipokines and PPARγ Levels.

    Science.gov (United States)

    Liu, Jinfeng; Dong, Huansheng; Zhang, Yong; Cao, Mingjun; Song, Lili; Pan, Qingjie; Bulmer, Andrew; Adams, David B; Dong, Xiao; Wang, Hongjun

    2015-05-28

    Obesity can cause insulin resistance and type 2 diabetes. Moderate elevations in bilirubin levels have anti-diabetic effects. This study is aimed at determining the mechanisms by which bilirubin treatment reduces obesity and insulin resistance in a diet-induced obesity (DIO) mouse model. DIO mice were treated with bilirubin or vehicle for 14 days. Body weights, plasma glucose, and insulin tolerance tests were performed prior to, immediately, and 7 weeks post-treatment. Serum lipid, leptin, adiponectin, insulin, total and direct bilirubin levels were measured. Expression of factors involved in adipose metabolism including sterol regulatory element-binding protein (SREBP-1), insulin receptor (IR), and PPARγ in liver were measured by RT-PCR and Western blot. Compared to controls, bilirubin-treated mice exhibited reductions in body weight, blood glucose levels, total cholesterol (TC), leptin, total and direct bilirubin, and increases in adiponectin and expression of SREBP-1, IR, and PPARγ mRNA. The improved metabolic control achieved by bilirubin-treated mice was persistent: at two months after treatment termination, bilirubin-treated DIO mice remained insulin sensitive with lower leptin and higher adiponectin levels, together with increased PPARγ expression. These results indicate that bilirubin regulates cholesterol metabolism, adipokines and PPARγ levels, which likely contribute to increased insulin sensitivity and glucose tolerance in DIO mice.

  10. The kinetics of oxidation of bilirubin and ascorbic acid in solution

    Science.gov (United States)

    Solomonov, A. V.; Rumyantsev, E. V.; Kochergin, B. A.; Antina, E. V.

    2012-07-01

    The results of a comparative study of the oxidation of bilirubin, ascorbic acid, and their mixture in aqueous solutions under the action of air oxygen and hydrogen peroxide are presented. The observed and true rate constants for the oxidation reactions were determined. It was shown that the oxidation of tetrapyrrole pigment occurred under these conditions bypassing the stage of biliverdin formation to monopyrrole products. Simultaneous oxidation of bilirubin and ascorbic acid was shown to be accompanied by the inhibition of ascorbic acid oxidation by bilirubin, whereas ascorbic acid itself activated the oxidation of bilirubin.

  11. Unconjugated bilirubin mediates heme oxygenase-1-induced vascular benefits in diabetic mice.

    Science.gov (United States)

    Liu, Jian; Wang, Li; Tian, Xiao Yu; Liu, Limei; Wong, Wing Tak; Zhang, Yang; Han, Quan-Bin; Ho, Hing-Man; Wang, Nanping; Wong, Siu Ling; Chen, Zhen-Yu; Yu, Jun; Ng, Chi-Fai; Yao, Xiaoqiang; Huang, Yu

    2015-05-01

    Heme oxygenase-1 (HO-1) exerts vasoprotective effects. Such benefit in diabetic vasculopathy, however, remains unclear. We hypothesize that bilirubin mediates HO-1-induced vascular benefits in diabetes. Diabetic db/db mice were treated with hemin (HO-1 inducer) for 2 weeks, and aortas were isolated for functional and molecular assays. Nitric oxide (NO) production was measured in cultured endothelial cells. Hemin treatment augmented endothelium-dependent relaxations (EDRs) and elevated Akt and endothelial NO synthase (eNOS) phosphorylation in db/db mouse aortas, which were reversed by the HO-1 inhibitor SnMP or HO-1 silencing virus. Hemin treatment increased serum bilirubin, and ex vivo bilirubin treatment improved relaxations in diabetic mouse aortas, which was reversed by the Akt inhibitor. Biliverdin reductase silencing virus attenuated the effect of hemin. Chronic bilirubin treatment improved EDRs in db/db mouse aortas. Hemin and bilirubin reversed high glucose-induced reductions in Akt and eNOS phosphorylation and NO production. The effect of hemin but not bilirubin was inhibited by biliverdin reductase silencing virus. Furthermore, bilirubin augmented EDRs in renal arteries from diabetic patients. In summary, HO-1-induced restoration of endothelial function in diabetic mice is most likely mediated by bilirubin, which preserves NO bioavailability through the Akt/eNOS/NO cascade, suggesting bilirubin as a potential therapeutic target for clinical intervention of diabetic vasculopathy.

  12. 21 CFR 862.1113 - Bilirubin (total and unbound) in the neonate test system.

    Science.gov (United States)

    2010-04-01

    ... HUMAN SERVICES (CONTINUED) MEDICAL DEVICES CLINICAL CHEMISTRY AND CLINICAL TOXICOLOGY DEVICES Clinical Chemistry Test Systems § 862.1113 Bilirubin (total and unbound) in the neonate test system....

  13. 21 CFR 862.1115 - Urinary bilirubin and its conjugates (nonquantitative) test system.

    Science.gov (United States)

    2010-04-01

    ... HEALTH AND HUMAN SERVICES (CONTINUED) MEDICAL DEVICES CLINICAL CHEMISTRY AND CLINICAL TOXICOLOGY DEVICES Clinical Chemistry Test Systems § 862.1115 Urinary bilirubin and its conjugates (nonquantitative)...

  14. Bilirubin as an antioxidant: kinetic studies of the reaction of bilirubin with peroxyl radicals in solution, micelles, and lipid bilayers.

    Science.gov (United States)

    Hatfield, Gillian L; Barclay, L Ross C

    2004-05-13

    Bilirubin (BR) showed very weak antioxidant activity in a nonpolar medium of styrene or cumene in chlorobenzene. In contrast, BR exhibited strong antioxidant activity in polar media such as aqueous lipid bilayers or SDS micelles/methyl linoleate (pH 7.4), where the rate with peroxyl radicals, k(inh) = 5.0 x 10(4) M(-)(1) s(-)(1), was comparable to that with vitamin E analogues, Trolox, or PMHC. An electron-transfer mechanism accounts for the effect of the medium on the antioxidant properties of BR.

  15. Biochemical measurement of bilirubin with an evanescent wave optical sensor

    Science.gov (United States)

    Poscio, Patrick; Depeursinge, Christian D.; Emery, Y.; Parriaux, Olivier M.; Voirin, Guy

    1991-09-01

    Optical sensing techniques can be considered as powerful information sources on the biochemistry of tissue, blood, and physiological fluids. Various sensing modalities can be considered: spectroscopic determination of the fluorescence or optical absorption of the biological medium itself, or more generally, of a reagent in contact with the biological medium. The principle and realization of the optical sensor developed are based on the use of polished fibers: the cladding of a monomode fiber is removed on a longitudinal section. The device can then be inserted into an hypodermic needle for in-vivo measurements. Using this minute probe, local measurements of the tissue biochemistry or metabolic processes can be obtained. The sensing mechanism is based on the propagation of the evanescent wave in the tissues or reagent: the proximity of the fiber core allows the penetration of the model field tail into the sensed medium, with a uniquely defined field distribution. Single or multi-wavelength analysis of the light collected into the fiber yields the biochemical information. Here an example of this sensing technology is discussed. In-vitro measurement of bilirubin in gastric juice demonstrates that the evanescent wave optical sensor provides a sensitivity which matches the physiological concentrations. A device is proposed for in-vivo monitoring of bilirubin concentration in the gastro-oesophageal tract.

  16. Bilirubin inhibits iNOS expression and NO production in response to endotoxin in rats.

    Science.gov (United States)

    Wang, Weizheng W; Smith, Darcey L H; Zucker, Stephen D

    2004-08-01

    The inducible isoform of heme oxygenase (HO), HO-1, has been shown to play an important role in attenuating tissue injury. Because HO-1 catalyzes the rate-limiting step in bilirubin synthesis, we examined the hypothesis that bilirubin is a key mediator of HO-1 cytoprotection, employing a rat model of endotoxemia. Bilirubin treatment resulted in improved survival and attenuated liver injury in response to lipopolysaccharide infusion. Serum levels of NO and tumor necrosis factor alpha, key mediators of endotoxemia, and hepatic inducible nitric oxide synthase (iNOS) expression were significantly lower in bilirubin-treated rodents versus control animals. Both intraperitoneal and local administration of bilirubin also was found to ameliorate hindpaw inflammation induced by the injection of lambda-carrageenan. Consistent with in vivo results, bilirubin significantly inhibited iNOS expression and suppressed NO production in lipopolysaccharide (LPS)-stimulated RAW 264.7 murine macrophages. In contrast, bilirubin treatment induced a threefold increase in LPS-mediated prostaglandin synthesis in the absence of significant changes in cyclooxygenase expression or activity, suggesting that bilirubin enhances substrate availability for eicosanoid synthesis. Bilirubin had no effect on LPS-mediated activation of nuclear factor kappaB or p38 mitogen-activated protein kinase, consistent with a nuclear factor kappaB-independent mechanism of action. Taken together, these data support a cytoprotective role for bilirubin that is mediated, at least in part, through the inhibition of iNOS expression and, potentially, through stimulation of local prostaglandin E2 production. In conclusion, our findings suggest a role for bilirubin in mollifying tissue injury in response to inflammatory stimuli and support the possibility that the phenomenon of "jaundice of sepsis" represents an adaptive physiological response to endotoxemia. Supplementary material for this article can be found on the

  17. Stopped-flow studies of spectral changes in bilirubin-human serum albumin following an alkaline pH jump and following binding of bilirubin

    DEFF Research Database (Denmark)

    Honoré, B

    1987-01-01

    ). The changes were analyzed according to a scheme of consecutive unimolecular reactions. Spectral monitoring of a pH jump from 11.3 to 11.8 reveals that the bilirubin-albumin complex changes its structure in several steps. The UV absorption spectra show that 3.8 tyrosine residues ionize in the first step, 2.......5 in the second, none in the third, and 0.8 in the fourth and following steps. The visible absorption spectrum of bound bilirubin changes in the second, third, and fourth steps. The bilirubin spectra of the different bilirubin-albumin complexes occurring in the transition show a common isosbestic point at 445 nm...

  18. Clinical system model for monitoring the physiological status of jaundice by extracting bilirubin components from skin diffuse reflectance spectra

    Science.gov (United States)

    Kumar, Alla S.; Clark, Joseph; Beyette, Fred R., Jr.

    2009-02-01

    Neonatal jaundice is a medical condition which occurs in newborns as a result of an imbalance between the production and elimination of bilirubin. The excess bilirubin in the blood stream diffuses into the surrounding tissue leading to a yellowing of the skin. As the bilirubin levels rise in the blood stream, there is a continuous exchange between the extra vascular bilirubin and bilirubin in the blood stream. Exposure to phototherapy alters the concentration of bilirubin in the vascular and extra vascular regions by causing bilirubin in the skin layers to be broken down. Thus, the relative concentration of extra vascular bilirubin is reduced leading to a diffusion of bilirubin out of the vascular region. Diffuse reflectance spectra from human skin contains physiological and structural information of the skin and nearby tissue. A diffuse reflectance spectrum must be captured before and after blanching in order to isolate the intravascular and extra vascular bilirubin. A new mathematical model is proposed with extra vascular bilirubin concentration taken into consideration along with other optical parameters in defining the diffuse reflectance spectrum from human skin. A nonlinear optimization algorithm has been adopted to extract the optical properties (including bilirubin concentration) from the skin reflectance spectrum. The new system model and nonlinear algorithm have been combined to enable extraction of Bilirubin concentrations within an average error of 10%.

  19. Ammonia-induced energy disorders interfere with bilirubin metabolism in hepatocytes.

    Science.gov (United States)

    Wang, Qiongye; Wang, Yanfang; Yu, Zujiang; Li, Duolu; Jia, Bin; Li, Jingjing; Guan, Kelei; Zhou, Yubing; Chen, Yanling; Kan, Quancheng

    2014-08-01

    Hyperammonemia and jaundice are the most common clinical symptoms of hepatic failure. Decreasing the level of ammonia in the blood is often accompanied by a reduction in bilirubin in patients with hepatic failure. Previous studies have shown that hyperammonemia can cause bilirubin metabolism disorders, however it is unclear exactly how hyperammonemia interferes with bilirubin metabolism in hepatocytes. The purpose of the current study was to determine the mechanism or mechanisms by which hyperammonemia interferes with bilirubin metabolism in hepatocytes. Cell viability and apoptosis were analyzed in primary hepatocytes that had been exposed to ammonium chloride. Mitochondrial morphology and permeability were observed and analyzed, intermediates of the tricarboxylic acid (TCA) cycle were determined and changes in the expression of enzymes related to bilirubin metabolism were analyzed after ammonia exposure. Hyperammonemia inhibited cell growth, induced apoptosis, damaged the mitochondria and hindered the TCA cycle in hepatocytes. This led to a reduction in energy synthesis, eventually affecting the expression of enzymes related to bilirubin metabolism, which then caused further problems with bilirubin metabolism. These effects were significant, but could be reversed with the addition of adenosine triphosphate (ATP). This study demonstrates that ammonia can cause problems with bilirubin metabolism by interfering with energy synthesis.

  20. Relationship of Bilirubin Levels in Infancy to Later Intellectual Development. Interim Report No. 20.

    Science.gov (United States)

    Rubin, Rosalyn A.; And Others

    The relationship of bilirubin (a red bile pigment that is sometimes found in the urine and occurs in the blood and tissues in jaundice) in infancy to later intellectual development was investigated in 241 infants with moderately elevated and high bilirubin levels. Ss were administered motor, psycholinguistic, and intelligence tests at age 8…

  1. Circulating Total Bilirubin and Risk of Incident Cardiovascular Disease in the General Population

    NARCIS (Netherlands)

    Kunutsor, Setor K.; Bakker, Stephan J. L.; Gansevoort, Ronald T.; Chowdhury, Rajiv; Dullaart, Robin P. F.

    2015-01-01

    OBJECTIVE: To assess the association of circulating total bilirubin and cardiovascular disease (CVD) risk in a new prospective study and to determine whether adding information on total bilirubin values to established cardiovascular risk factors is associated with improvement in prediction of CVD ri

  2. New Sorbent for Bilirubin Removal from Human Plasma: Albumin Immobilized Microporous Membranous PTFE Capillaries

    Institute of Scientific and Technical Information of China (English)

    Lei ZHANG; Gu JIN

    2005-01-01

    In this study, we developed a tailored capillary sorbent for bilirubin removal. For immobilized bioligand, capillaries were grafted with epoxy groups using RIGP. The HSA immobilized capillaries has a high affinity adsorption capacity (71.2 mg bilirubin/g polymer) and a shorter adsorption equilibrium time (about 60 min).

  3. Association of bilirubin and protein thiols in relation to copper and ceruloplasmin in hyperbilirubinemic patients

    Institute of Scientific and Technical Information of China (English)

    Mungli Prakash; Jeevan K Shetty; Roshan D'Souza; Suhasa Upadhya; Vijay Kumar

    2009-01-01

    Objective:Bilirubin is a double edged sword in biological system,acting as a toxic molecule and cytoprotecrant.Unconjugated bilirubin is proved to show antioxidant activity in vitro and in vivo.In the current work we tried to know the relationship between both conjugated and unconjugated bilirubin with copper and protein thiols in patients with hyperbilirnbinemia.Methods:Study was conducted on 56 hyperbilirubinemic cases and 56 healthy controls.Serum copper,ceruloplasmin,protein thiols,total bilirubin,conjugated and unconjugated bilirubin,unconjugated bilimbin/albumin ratio,total protein,albumin,AST,ALT and ALP were estimated.Results:There was significant increase in serum copper,total bilirubin,conjugated and unconjugated bilimbin.uriconjugated bilirubin/albumin ratio,AST,ALT,and ALP,and decrease in serum ceruloplasmin,protein thiols,total protein,and albumin in hyperbilimbinemic cases when compared to healthy controls.Conjugated bilimbin correlated positively with liver enzymes AST and ALP,and negatively with protein thials,total protein and albumin.Unconjugated bilirubin correlated positively with ALT.Protein thiols correlated negatively with copper and positively with ceruloplasmin,and also correlated negativelv with liver enzymes like AST,ALT and ALP,and positively with total protein and albumin.Conclusion:Combination of elevated levels of trace elements like copper and availability of reducing agent like bilimbin may prove deleterious by generating free radicals.

  4. EXPERIMENTAL AND CLINICAL STUDY ON BILIRUBIN NEUROTOXICITY DETECTED BY VISUAL EVOKED POTENTIALS TO FLASH

    Institute of Scientific and Technical Information of China (English)

    贲晓明; 秦玉明; 吴圣楣; 张惠民; 陈舜年; 夏振炜

    2001-01-01

    Objective Evaluate the sensitivity and reliability of visual evoked potential to flash ( FVEP ) in detecting bilirubin neurotoxicity and approach the risk parameters of bilirubin neurotoxicity in hyperbilirubinernia newborns. Methods Based on the successful establishment of animal models for acute bilirubin encephalopathy by intraperitoneal infusion of bilirubin with a dosage of 100~200μg /g body weight to 1-weekold guinea pigs, the F-VEP was recorded in animal models and human neonates with hyperbilirubinemia, and the sensitivity and reliability of F-VEP in detecting bilirubin neurotoxicity were evaluated. Results F-VEP features and its P1 latency significantly correlated to brain adenosine triphosphate (ATP) level, neurobehavioral and neuropathological changes in experimental bilirubin encephalopathy ; neonates with hyperbilirubinemia showed significant F-VEP changes characterized by absence of P1 or P1 latency prolonged in 1~7-dayold newborns, especially when the jaundice was caused by immunoincompatibility and infectious diseases. Conclusion F-VEP would be a good discriminator for bilirubin neurotoxicity, and can become a promising technique in monitoring bilirubin encephalopathy.

  5. Effect of bilirubin on triglyceride synthesis in streptozotocin-induced diabetic nephropathy.

    Science.gov (United States)

    Xu, Jianwei; Lee, Eun Seong; Baek, Seon Ha; Ahn, Shin-Young; Kim, Sejoong; Na, Ki Young; Chae, Dong-Wan; Chin, Ho Jun

    2014-09-01

    We aimed to elucidate the effect of bilirubin on dyslipidemia and nephropathy in a diabetes mellitus (DM) type I animal model. Sprague-Dawley rats were separated into control, DM, and bilirubin-treated DM (Bil) groups. The Bil group was injected intraperitoneally with 60 mg/kg bilirubin 3 times per week and hepatoma cells were cultured with bilirubin at a concentration of 0.3 mg/dL. The Bil group showed lower serum creatinine levels 5 weeks after diabetes onset. Bilirubin treatment also decreased the amount of mesangial matrix, lowered the expression of renal collagen IV and transforming growth factor (TGF)-β1, and reduced the level of apoptosis in the kidney, compared to the DM group. These changes were accompanied by decreased tissue levels of hydrogen superoxide and NADPH oxidase subunit proteins. Bilirubin decreased serum total cholesterol, high-density lipoprotein cholesterol (HDL-C), free fatty acids, and triglycerides (TGs), as well as the TG content in the liver tissues. Bilirubin suppressed protein expression of LXRα, SREBP-1, SCD-1, and FAS, factors involved in TG synthesis that were elevated in the livers of DM rats and hepatoma cells under high-glucose conditions. In conclusion, bilirubin attenuates renal dysfunction and dyslipidemia in diabetes by suppressing LXRα and SREBP-1 expression and oxidative stress.

  6. Purification, characterization and decolorization of bilirubin oxidase from Myrothecium verrucaria 3.2190

    Science.gov (United States)

    Myrothecium verrucaria 3.2190 is a nonligninolytic fungus that produces bilirubin oxidase. Both Myrothecium verrucaria and the extracellular bilirubin oxidase were tested for their ability to decolorize indigo carmine. The biosorption and biodegradation of the dye were detected during the process of...

  7. Multiple binding of bilirubin to human serum albumin and cobinding with laurate

    DEFF Research Database (Denmark)

    Sato, H; Honoré, B; Brodersen, R

    1988-01-01

    Numerical analysis of multiple binding of two ligands to one carrier has been accomplished, using the principle of several sets of acceptable binding constants, with bilirubin-laurate-albumin as an example. Binding of bilirubin to defatted human serum albumin was investigated by a spectroscopic m...

  8. Thyroid hormone uptake in cultured rat anterior pituitary cells: effects of energy status and bilirubin

    NARCIS (Netherlands)

    F.W.J.S. Wassen (Frank); E.P.C.M. Moerings (Ellis); H. van Toor (Hans); G. Hennemann; M.E. Everts (Maria)

    2000-01-01

    textabstractTransport of thyroxine (T(4)) into the liver is inhibited in fasting and by bilirubin, a compound often accumulating in the serum of critically ill patients. We tested the effects of chronic and acute energy deprivation, bilirubin and its precursor biliverdi

  9. Metabolism of bilirubin by human cytochrome P450 2A6

    Energy Technology Data Exchange (ETDEWEB)

    Abu-Bakar, A' edah, E-mail: a.abubakar@uq.edu.au [The University of Queensland, National Research Centre for Environmental Toxicology (Entox), 4072 Brisbane, Queensland (Australia); Arthur, Dionne M. [The University of Queensland, National Research Centre for Environmental Toxicology (Entox), 4072 Brisbane, Queensland (Australia); Cooperative Research Centre for Contamination Assessment and Remediation of the Environment, Adelaide (Australia); Wikman, Anna S. [The University of Queensland, National Research Centre for Environmental Toxicology (Entox), 4072 Brisbane, Queensland (Australia); Department of Pharmaceutical Biosciences, Uppsala University, SE-75123 Uppsala (Sweden); Rahnasto, Minna; Juvonen, Risto O.; Vepsäläinen, Jouko; Raunio, Hannu [School of Pharmacy, Faculty of Health Sciences, University of Eastern Finland, POB 1627, 70211 Kuopio (Finland); Ng, Jack C. [The University of Queensland, National Research Centre for Environmental Toxicology (Entox), 4072 Brisbane, Queensland (Australia); Cooperative Research Centre for Contamination Assessment and Remediation of the Environment, Adelaide (Australia); Lang, Matti A. [The University of Queensland, National Research Centre for Environmental Toxicology (Entox), 4072 Brisbane, Queensland (Australia)

    2012-05-15

    The mouse cytochrome P450 (CYP) 2A5 has recently been shown to function as hepatic “Bilirubin Oxidase” (Abu-Bakar, A., et al., 2011. Toxicol. Appl. Pharmacol. 257, 14–22). To date, no information is available on human CYP isoforms involvement in bilirubin metabolism. In this paper we provide novel evidence for human CYP2A6 metabolising the tetrapyrrole bilirubin. Incubation of bilirubin with recombinant yeast microsomes expressing the CYP2A6 showed that bilirubin inhibited CYP2A6-dependent coumarin 7-hydroxylase activity to almost 100% with an estimated K{sub i} of 2.23 μM. Metabolite screening by a high-performance liquid chromatography/electrospray ionisation mass spectrometry indicated that CYP2A6 oxidised bilirubin to biliverdin and to three other smaller products with m/z values of 301, 315 and 333. Molecular docking analyses indicated that bilirubin and its positively charged intermediate interacted with key amino acid residues at the enzyme's active site. They were stabilised at the site in a conformation favouring biliverdin formation. By contrast, the end product, biliverdin was less fitting to the active site with the critical central methylene bridge distanced from the CYP2A6 haem iron facilitating its release. Furthermore, bilirubin treatment of HepG2 cells increased the CYP2A6 protein and activity levels with no effect on the corresponding mRNA. Co-treatment with cycloheximide (CHX), a protein synthesis inhibitor, resulted in increased half-life of the CYP2A6 compared to cells treated only with CHX. Collectively, the observations indicate that the CYP2A6 may function as human “Bilirubin Oxidase” where bilirubin is potentially a substrate and a regulator of the enzyme. -- Highlights: ► Human CYP2A6 interacts with bilirubin with a high affinity. ► Bilirubin docking to the CYP2A6 active site is more stable than biliverdin docking. ► Recombinant CYP2A6 microsomes metabolised bilirubin to biliverdin. ► Bilirubin increased the hepatic

  10. Increased conjugated bilirubin is sufficient to initiate screening for biliary atresia

    DEFF Research Database (Denmark)

    Madsen, Stine Skipper; Kvist, Nina; Thorup, Jørgen

    2015-01-01

    cirrhosis. The Danish Health and Medicines Authority (DHMA) demands diagnostic evaluation of children with elevated level of serum bilirubin after two weeks of age. Biliary atresia has to be excluded if conjugated bilirubin level is above than 20 μmol/l, and/or more than 20% of total bilirubin......: During the period, 73 patients where operated with a portoenterostomy ad modum Kasai. Patients older than 84 days at the time of operation were excluded, 54 patients were available for analysis. Conjugated bilirubin in μmol/l and the percentage value were significantly above the DHMA threshold limit......: mean 129.7 μmol/l (42-334 μmol/l) and 73% (28-97%), respectively. CONCLUSION: The total amount of conjugated bilirubin above 20 μmol/l is sufficient to require further evaluation for biliary atresia. The percentage value is unnecessary and may cause confusion. FUNDING: none. TRIAL REGISTRATION...

  11. Increased large VLDL and small LDL particles are related to lower bilirubin in Type 2 diabetes mellitus

    NARCIS (Netherlands)

    Dullaart, Robin P F; de Vries, Rindert; Lefrandt, Joop D

    2014-01-01

    OBJECTIVES: Bilirubin may protect against atherosclerotic cardiovascular disease by virtue of its anti-oxidative properties, but lower bilirubin may also be associated to atherogenic lipoprotein abnormalities. We determined associations of plasma (apo)lipoproteins and lipoprotein subfractions in sub

  12. Primary Biliary Cirrhosis and Hemolytic Anemia Confusing Serum Bilirubin Levels

    Directory of Open Access Journals (Sweden)

    M Brackstone

    2000-01-01

    Full Text Available Hemolysis is observed in more than 50% of patients with cirrhosis. However, there has been little documention of the association of primary biliary cirrhosis with autoimmune hemolytic anemia. Two cases, found within a single practice, of primary biliary cirrhosis coexisting with autoimmune hemolysis and a third case coexisting with hereditary spherocytosis are presented. Anemia in such patients is commonly attributed to chronic disease, and hyperbilirubinemia is attributed to primary biliary cirrhosis. These patients were considered for liver transplantation until the diagnosis of a comorbid hemolytic process was established. This association may be more prevalent than previously recognized. A diagnosis of comorbid hemolysis must always be considered in context with anemia and serum bilirubin levels that rise out of proportion to the severity of the primary biliary cirrhosis.

  13. Bilirubin and Progression of Nephropathy in Type 2 Diabetes : A Post Hoc Analysis of RENAAL With Independent Replication in IDNT

    NARCIS (Netherlands)

    Riphagen, Ineke J.; Deetman, Petronella E.; Bakker, Stephan J. L.; Navis, Gerarda; Cooper, Mark E.; Lewis, Julia B.; de Zeeuw, Dick; Lambers Heerspink, Hiddo

    2014-01-01

    Bilirubin, a potent endogenous antioxidant, was found to protect against the development of diabetic nephropathy (DN) in rodents. In humans, cross-sectional studies found an inverse relation between bilirubin and DN. We prospectively investigated whether bilirubin is associated with progression of D

  14. Statins alter the hepatobiliary transport of unconjugated and conjugated bilirubin in sandwich-cultured rat hepatocytes.

    Science.gov (United States)

    Szabó, Mónika; Veres, Zsuzsa; Bátai-Konczos, Attila; Kékesi, Orsolya; Kis, Emese; Szabó, Kitti; Jemnitz, Katalin

    2014-09-01

    Several studies have reported that statins occasionally cause impairment of liver functions characterized by elevated serum bilirubin levels, which might be due to altered function of the multidrug resistance-associated proteins (Mrp2/3). We aimed to study the modulation of the hepatobiliary transport of bilirubin by four statin derivatives, atorvastatin, fluvastatin, pravastatin, and rosuvastatin in sandwich-cultured rat hepatocytes. All statins except pravastatin significantly inhibited the uptake of bilirubin. The biliary efflux of bilirubin conjugates was increased by pravastatin and rosuvastatin concentration dependently. Rosuvastatin stimulated not only the Mrp2 mediated biliary, but the Mrp3 mediated sinusoidal elimination, resulting in decreased intracellular bilirubin accumulation. The significantly induced Mrp2/3 protein levels (ranging from 1.5 to 1.8-fold) accounted for the elevated efflux. Cell polarization, the formation of biliary network was also significantly increased by fluvastatin, pravastatin and rosuvastatin (151%, 216% and 275% of the control, respectively). The simultaneous inhibition of the uptake and the stimulation of the sinusoidal and canalicular elimination may explain, at least in part, the clinical observation of elevated serum bilirubin levels. In conclusion, our results suggest that in spite of the elevated serum bilirubin levels, the altered Mrp2 and Mrp3 functions by statins is probably not associated with hepatotoxic effects.

  15. [Haemolysis and turbidity influence on three analysis methods of quantitative determination of total and conjugated bilirubin on ADVIA 1650].

    Science.gov (United States)

    Gobert De Paepe, E; Munteanu, G; Schischmanoff, P O; Porquet, D

    2008-01-01

    Plasma bilirubin testing is crucial to prevent the occurrence of neonatal kernicterus. Haemolysis may occur during sampling and interfere with bilirubin determination. Moreover, lipidic infusions may induce plasma lipemia and also interfere with bilirubin measurement. We evaluated the interference of haemolysis and lipemia with three methods of total and direct bilirubin measurement adaptated on an Advia 1650 analyser (Siemens Medical Solutions Diagnostics) : Synermed (Sofibel), Bilirubin 2 (Siemens) and Bilirubin Auto FS (Diasys). The measurement of total bilirubin was little affected by haemolysis with all three methods. The Bilirubin 2 (Siemens) method was the less sensitive to haemolysis even at low bilirubin levels. The measurement of conjugated bilirubin was significantly altered by low heamoglobin concentrations for Bilirubin Auto FS(R) (30 microM or 0,192 g/100 mL haemoglobin) and for Synermed (60 microM or 0,484 g/100 mL haemoglobin). In marked contrast, we found no haemoglobin interference with the Direct Bilirubin 2 reagent which complied with the method validation criteria from the French Society for Biological Chemistry. The lipemia up to 2 g/L of Ivelip did not affect neither the measurement of total bilirubin for all three methods nor the measurement of conjugated bilirubin with the Diasys and Siemens reagents. However, we observed a strong interference starting at 0,5 g/L of Ivelip with the Synermed reagent. Our data suggest that both Siemens and Diasys methods allow to measure accurately total and conjugated bilirubin in hemolytic and lipemic samples, nevertheless, the Siemens methodology is less affected by these interferences.

  16. Intraperitoneal Bilirubin Administration Decreases Infarct Area in a Rat Coronary Ischemia/Reperfusion Model

    Directory of Open Access Journals (Sweden)

    Ron eBen-Amotz

    2014-02-01

    Full Text Available Bilirubin was previously considered a toxin byproduct of heme catabolism. However, a mounting body of evidence suggests that at physiological doses, bilirubin is a powerful antioxidant and anti-atherosclerotic agent. Recent clinical studies have shown that human beings with genetically-induced hyperbilirubinemia (Gilbert Syndrome are protected against coronary heart disease. The purpose of this study was to investigate whether administration of exogenous bilirubin to normal rats would convey similar protective effects in an experimental model of coronary ischemia. We hypothesized that intraperitoneal bilirubin administration 1 hour before injury would decrease infarct area and preserve left ventricular (LV systolic function when compared to non-treated rats. Coronary ischemia was induced by temporary (30 min ligation of the left anterior descending coronary artery in control or bilirubin treated rats, followed by a 1-hour period of reperfusion. LV function was estimated by measurements of fractional shortening and fractional area shortening using echocardiography. LV function decreased in both experimental groups after ischemia and reperfusion, although in bilirubin-treated rats fractional shortening was less depressed during the period of ischemia (18.8 vs 25.8%, p = 0.034. Infarct size was significantly reduced in the bilirubin treated group compared to the non-treated group (13.34% vs 25.5%, p = 0.0067. Based on the results of this study, bilirubin supplementation appears to provide significant decrease in infarct size although protective effects on LV function were noted only during the period of ischemia. This result also suggests that lipid soluble antioxidant bilirubin prevents the oxidation of cardiolipin and decreases the infarct size in the heart during ischemia.

  17. Relation of Pre-anthracycline Serum Bilirubin Levels to Left Ventricular Ejection Fraction After Chemotherapy.

    Science.gov (United States)

    Vera, Trinity; D'Agostino, Ralph B; Jordan, Jennifer H; Whitlock, Matthew C; Meléndez, Giselle C; Lamar, Zanetta S; Porosnicu, Mercedes; Bonkovsky, Herbert L; Poole, Leslie B; Hundley, W Gregory

    2015-12-01

    Myocardial injury because of oxidative stress manifesting through reductions in left ventricular ejection fraction (LVEF) may occur after the administration of anthracycline-based chemotherapy (A-bC). We hypothesized that bilirubin, an effective endogenous antioxidant, may attenuate the reduction in LVEF that sometimes occurs after receipt of A-bC. We identified 751 consecutively treated patients with cancer who underwent a pre-A-bC LVEF measurement, exhibited a serum total bilirubin level bilirubin and LVEF changes. The LVEF decreased by 10.7 ± 13.7%, 8.9 ± 11.8%, and 7.7 ± 11.5% in group 1 (bilirubin at baseline ≤0.5 mg/dl), group 2 (bilirubin 0.6 to 0.8 mg/dl), and group 3 (bilirubin 0.9 to 1.9 mg/dl), respectively. More group 1 patients experienced >15% decrease in LVEF compared with those in group 3 (p = 0.039). After adjusting for age, coronary artery disease/myocardial infarction, diabetes mellitus, hematocrit, and the use of cardioactive medications, higher precancer treatment bilirubin levels and lesser total anthracycline doses were associated with LVEF preservation (p = 0.047 and 0.011, respectively). In patients treated with anthracyclines who subsequently develop symptoms associated with heart failure, pre-anthracycline treatment serum bilirubin levels inversely correlate with subsequent deterioration in post-cancer treatment LVEF. In conclusion, these results suggest that increased levels of circulating serum total bilirubin, an intrinsic antioxidant, may facilitate preservation of LVEF in patients receiving A-bC for cancer.

  18. Cobinding of bilirubin and laurate to human serum albumin: spectroscopic characterization of stoichiometric complexes

    DEFF Research Database (Denmark)

    Honoré, B; Sato, H; Brodersen, R

    1988-01-01

    Light absorption and CD spectra of bound bilirubin and albumin fluorescence spectra have been recorded from mixtures containing albumin, A, bilirubin, B, and laurate, L, in Tris-NaCl buffer at pH 8.2, 25 degrees C. Concentrations of the corresponding stoichiometric complexes, ABiLj, for i = 0....... Brodersen et al. (1987) Eur. J. Biochem. 169, 487-495). The results were utilized at the microscopic level to investigate ligand-induced conformational changes. When laurate was bound to AB, a decrease of the distance between Trp-214 and the bound bilirubin occurred, as measured according to Förster...

  19. Enhanced bilirubin binding to different mammalian erythrocytes in the presence of magnesium ions

    OpenAIRE

    M. K. Ali; Siddiqui, M. U.; Tayyab, S.

    2001-01-01

    Effect of magnesium ions on the binding of bilirubin to erythrocytes of different mammalian species, namely, human, buffalo, goat and sheep was studied. Increase in the concentration of magnesium ions led to a gradual increase in the erythrocyte-bound bilirubin in both human and buffalo erythrocytes whereas in sheep and goat erythrocytes, the pronounced increase was found beyond 2.0 and 2.7 mM MgCl2 concentrations respectively. Percentage increase in erythrocyte-bound bilirubin was found high...

  20. Preparation of Aminated Macroporous Polyvinyl Alcohol Resins and Evaluation for Bilirubin Adsorption

    Institute of Scientific and Technical Information of China (English)

    WANG Wei-chao; ZHANG Sheng-nan; HU Yue-han; XIE Hui; OU Lai-liang; YU Yao-ting; KONG De-ling; GU Han-qing

    2008-01-01

    In the present study we prepared macroporous polyvinyl alcohol beads. A series of bilirubin adsorbents were generated by immobilization of eight amine agents to the beads as ligands. The adsorption of bilirubin was evaluated by in vitro static and dynamic adsorption tests. The results show that these adsorbents have excellent adsorption efficiency and capacity. Among the eight ligands, trimethylamine (TMA), triethylamine (TEA) and 1,6- hexanediamine(HAD) showed the highest adsorption capacity. The adsorption equilibrium can be achieved in half an hour, and the adsorption percentage of bilirubin was up to 80%. Static electricity and hydrophobic interaction played the main role in bilirubin adsorption, and the adsorption was found to match the monolayer model. The excellent adsorption of these adsorbents indicates their potential in clinical treatment.

  1. Computational chemical analysis of unconjugated bilirubin anions and insights into pKa values clarification

    Science.gov (United States)

    Vega-Hissi, Esteban G.; Estrada, Mario R.; Lavecchia, Martín J.; Pis Diez, Reinaldo

    2013-01-01

    The pKa, the negative logarithm of the acid dissociation equilibrium constant, of the carboxylic acid groups of unconjugated bilirubin in water is a discussed issue because there are quite different experimental values reported. Using quantum mechanical calculations we have studied the conformational behavior of unconjugated bilirubin species (in gas phase and in solution modeled implicitly and explicitly) to provide evidence that may clarify pKa values because of its pathophysiological relevance. Our results show that rotation of carboxylate group, which is not restricted, settles it in a suitable place to establish stronger interactions that stabilizes the monoanion and the dianion to be properly solvated, demonstrating that the rationalization used to justify the high pKa values of unconjugated bilirubin is inappropriate. Furthermore, low unconjugated bilirubin (UCB) pKa values were estimated from a linear regression analysis.

  2. Unilobar versus bilobar biliary drainage: effect on quality of life and bilirubin level reduction

    Directory of Open Access Journals (Sweden)

    Shivanand Gamanagatti

    2016-01-01

    Conclusion: Percutaneous biliary drainage provides good palliation of malignant obstructive jaundice. Partial-liver drainage achieved results as good as those after complete liver drainage with significant improvements in QOL and reduction of the bilirubin level.

  3. The effect of bilirubin on lipid peroxidation and antioxidant enzymes in cumene hydroperoxide-treated erythrocytes.

    Science.gov (United States)

    Yeşilkaya, A; Yeğin, A; Ozdem, S; Aksu, T A

    1998-01-01

    Recently, it has been suggested that bilirubin may act as a potent biological chain-breaking antioxidant. To observe the effects of free bilirubin on antioxidant reactions in cumene hydroperoxide-treated erythrocytes (15 g hemoglobin/dl), we added bilirubin at four different concentrations (0.5, 1, 5, and 10 mg/dl). We measured the thiobarbituric acid-reactive substance and reduced glutathione levels, and some antioxidant enzyme activities, namely superoxide dismutase, catalase, and glucose-6-phosphate dehydrogenase. Thiobarbituric acid-reactive substance and chemiluminescent signals decreased during the incubation. Superoxide dismutase activities also decreased but not as much as in the control group. Glucose-6-phosphate dehydrogenase activities and reduced glutathione levels increased, but catalase activities remained the same as the control group. Our results suggest that bilirubin--in the concentrations we have used--partially prevented the oxidant effects of cumene hydroperoxide.

  4. Serum bilirubin levels, UGT1A1 polymorphisms and risk for coronary artery disease.

    Science.gov (United States)

    Lingenhel, Arno; Kollerits, Barbara; Schwaiger, Johannes P; Hunt, Steven C; Gress, Richard; Hopkins, Paul N; Schoenborn, Veit; Heid, Iris M; Kronenberg, Florian

    2008-12-01

    Low levels of the antioxidative serum bilirubin are associated with vascular aging and an increased risk for coronary artery disease (CAD). UGT1A1 is the major gene influencing bilirubin concentrations. Therefore, we investigated an association of bilirubin levels and two polymorphisms in the promoter of UGT1A1 (-53(TA-repeat) polymorphism and T-3279G) in 477 patients with premature, familial CAD and 619 age- and sex-matched controls. Bilirubin concentrations were significantly lower in cases than in controls (0.62+/-0.36 vs. 0.76+/-0.41 mg/dl for men, p=1.2 x 10(-10); and 0.42+/-0.29 vs. 0.55+/-0.23 mg/dl, p=1.9 x 10(-9) for women). Both polymorphisms showed a strong association with bilirubin levels with higher levels for homozygote carriers of the minor allele. These associations were most pronounced in male controls and patients (p=5.9 x 10(-26) and p=3.4 x 10(-16), respectively, for the -53(TA-repeat) polymorphism). Logistic regression analysis revealed low bilirubin levels but not the UGT1A1 polymorphisms to be significantly associated with CAD: OR (95% CI) 0.90 (0.86-0.94), p=2.6 x 10(-6) for men and 0.77 (0.68-0.87), p=3.2 x 10(-5) for women, respectively for each 0.1mg/dl increase of bilirubin. These results indicate that it is rather decreased bilirubin levels in general than the changes in the genetic variation of this gene that increase the risk for CAD.

  5. Evaluation of oxidant and antioxidant status in term neonates: a plausible protective role of bilirubin.

    Science.gov (United States)

    Shekeeb Shahab, M; Kumar, Praveen; Sharma, Neeraj; Narang, Anil; Prasad, Rajendra

    2008-10-01

    In vitro studies have shown unequivocally that bilirubin is an antioxidant. We hypothesized that bilirubin serves a physiological role of an antioxidant in vivo. To investigate the probable protective role of bilirubin in vivo, term babies with clinical jaundice were grouped into four categories-serum total bilirubin (STB) 200 mg/l, and kernicterus. Serum bilirubin, serum albumin, plasma glucose-6-phosphate dehydrogenase (G6PD), lipid peroxidation in blood cells, and reduced glutathione (GSH) content in whole blood were investigated. We also measured superoxide dismutase (SOD) and catalase in hemolysate and total plasma antioxidant capacity (TAC). Lipid peroxidation and antioxidant enzymes were significantly lower in babies with STB 200 mg/l and in babies with bilirubin encephalopathy. Elevated levels of MDA, SOD, and catalase and significantly decreased levels of reduced glutathione and total antioxidant capacity were observed in STB >200 mg/l group. Antioxidant enzymes were also significantly inhibited in bilirubin encephalopathy babies. Post phototherapy, MDA production and antioxidant levels were significantly increased whilst total antioxidant capacity and reduced glutathione were significantly decreased compared to pre-phototherapy values. Exchange transfusion resulted in reduced oxidative stress in subjects with encephalopathy, whereas no significant difference was observed in other babies with STB >200 mg/l. Taken together, the present study propounds that bilirubin acts as a physiological antioxidant till 200 mg/l concentration in full-term normal neonates. It is conjectured that beyond 200 mg/l, it can no longer be considered physiologic. However, the cause of pathological jaundice needs to be identified and treated. The present data documents that phototherapy also induces oxidative stress.

  6. Studies on The Adsorption Capacity for Bilirubin of The Adsorbent Chitosan-β-Cyclodextrin

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    The adsorbent crosslinked chitosan-β-cyclodextrin (β-CD) was prepared by the reaction of glutaraldehyde with chitosan and β-cyclodextrin. This type of adsorbent has high adsorption capacity for unconjugated bilirubin. The adsorption capacity was related to the β-CD content of the adsorbent; phosphate buffer concentration; temperature; pH value; ionic strength and the adsorbent beads. The results indicated that the chitosan-β-CD was a good adsorbent for unconjugated bilirubin with high capacity.

  7. Quantitative determination of cerebrospinal fluid bilirubin on a high throughput chemistry analyzer

    OpenAIRE

    Said Ahmed, Degmo

    2009-01-01

    Background Subarachnoid hemorrhage is a condition with high rates of mortality and morbidity. The diagnosis requires an urgent cerebral computed tomography scan and also a lumbar puncture if the scan fails to demonstrate intracranial blood. In Sweden the cerebrospinal fluid (CSF) is analyzed by spectrophotometric scanning for the presence of hemoglobin and bilirubin. The aim of the study was to develop a quantitative diazo reagent based analysis of cerebrospinal fluid bilirubin as a replaceme...

  8. Successive determination of urinary bilirubin and creatinine employing simultaneous injection effective mixing flow analysis.

    Science.gov (United States)

    Ponhong, Kraingkrai; Teshima, Norio; Grudpan, Kate; Vichapong, Jitlada; Motomizu, Shoji; Sakai, Tadao

    2015-02-01

    A novel four-channel simultaneous injection effective mixing flow analysis (SIEMA) system has been assembled for successive determination of bilirubin and creatinine in urinary samples. The chemical variables and physical parameters in the flow system were optimized for the enhancement of successive analytical performances. The interferences from urine matrices on the determination of bilirubin and creatinine were eliminated to dilute urine samples. The calibration graphs with the optimum conditions were achieved to be in 0.024-5.0 mg L(-1) for bilirubin and 2-100 mg L(-1) for creatinine. The relative standard deviations (RSDs) at 3 mg L(-1) of bilirubin and at 50 mg L(-1) of creatinine for 11 runs were 1.5 and 1.0%, respectively. The limits of detections (3σ of blank) for bilirubin and creatinine were 7 µg L(-1) and 0.6 mg L(-1), respectively. The sample throughput for stepwise detection was 22 h(-1). The proposed method was applied to the successive determination of bilirubin and creatinine in urine samples.

  9. Development of a System Model for Non-Invasive Quantification of Bilirubin in Jaundice Patients

    Science.gov (United States)

    Alla, Suresh K.

    Neonatal jaundice is a medical condition which occurs in newborns as a result of an imbalance between the production and elimination of bilirubin. Excess bilirubin in the blood stream diffuses into the surrounding tissue leading to a yellowing of the skin. An optical system integrated with a signal processing system is used as a platform to noninvasively quantify bilirubin concentration through the measurement of diffuse skin reflectance. Initial studies have lead to the generation of a clinical analytical model for neonatal jaundice which generates spectral reflectance data for jaundiced skin with varying levels of bilirubin concentration in the tissue. The spectral database built using the clinical analytical model is then used as a test database to validate the signal processing system in real time. This evaluation forms the basis for understanding the translation of this research to human trials. The clinical analytical model and signal processing system have been successful validated on three spectral databases. First spectral database is constructed using a porcine model as a surrogate for neonatal skin tissue. Samples of pig skin were soaked in bilirubin solutions of varying concentrations to simulate jaundice skin conditions. The resulting skins samples were analyzed with our skin reflectance systems producing bilirubin concentration values that show a high correlation (R2 = 0.94) to concentration of the bilirubin solution that each porcine tissue sample is soaked in. The second spectral database is the spectral measurements collected on human volunteers to quantify the different chromophores and other physical properties of the tissue such a Hematocrit, Hemoglobin etc. The third spectral database is the spectral data collected at different time periods from the moment a bruise is induced.

  10. Serum total bilirubin levels and coronary heart disease--Causal association or epiphenomenon?

    Science.gov (United States)

    Kunutsor, Setor K

    2015-12-01

    Observational epidemiological evidence supports a linear inverse and independent association between serum total bilirubin levels and coronary heart disease (CHD) risk, but whether this association is causal remains to be ascertained. A Mendelian randomization approach was employed to test whether serum total bilirubin is causally linked to CHD. The genetic variant rs6742078--well known to specifically modify levels of serum total bilirubin and accounting for up to 20% of the variance in circulating serum total bilirubin levels--was used as an instrumental variable. In pooled analysis of estimates reported from published genome-wide association studies, every copy of the T allele of rs6742078 was associated with 0.42 standard deviation (SD) higher levels of serum total bilirubin (95% confidence interval, 0.40 to 0.43). Based on combined data from the Coronary Artery Disease Genome wide Replication and Meta-analyses and the Coronary Artery Disease (C4D) Genetics Consortium involving a total of 36,763 CHD cases and 76,997 controls, the odds ratio for CHD per copy of the T allele was 1.01 (95% confidence interval, 0.99 to 1.04). The odds ratio of CHD for a 1 SD genetically elevated serum total bilirubin level was 1.03 (95% confidence interval, 0.98 to 1.09). The current findings casts doubt on a strong causal association of serum total bilirubin levels with CHD. The inverse associations demonstrated in observational studies may be driven by biases such as unmeasured confounding and/or reverse causation. However, further research in large-scale consortia is needed.

  11. Predictors of the change in bilirubin levels over twelve weeks of treatment with atazanavir

    LENUS (Irish Health Repository)

    Cotter, Aoife G

    2013-05-16

    AbstractObjectiveTo determine the factors associated with change in bilirubin concentration 12 weeks after the initiation of an atazanavir (ATV)-containing antiretroviral regimen.MethodsWe performed a retrospective case note review of all patients prescribed ATV between January 2004 and October 2007 in a cohort of HIV infected subjects. Data collected included baseline demographics, hepatitis B and C serology, current antiretroviral therapy, baseline and week 12 routine bloods. The primary endpoint was the change in bilirubin concentration at 12 weeks after start of ATV. Multvariable linear regression was performed to assess the relationships between the change in bilirubin and variables of interest. Results: Eighty-three ATV-treated patients were included in the analysis of whom 46 (60.5%) were hepatitis C antibody positive. The median (interquartile range) change in bilirubin by week 12 was 16 (4, 22) umol\\/L; only 1 patient developed grade 4 hyperbilirubinaemia at week 12. After controlling for baseline bilirubin levels, HCV seropositivity and baseline ALP were associated with a smaller change in bilirubin over the 12 weeks with a trend towards lower increases in those receiving tenofovir. Sensitivity analyses reported similar associations with methadone use and injection drug use, when these variables replaced HCV sero-positivity in the model. Conclusion: Patients with hepatitis C co-infection experience smaller changes in bilirubin upon exposure to ATV. Although the underlying mechanism for this association remains unclear, these data support the safe use of this drug in this patient setting. Further research into the clinical predictors of ATV-related hyperbilirubinaemia is warranted.

  12. Study on the Property of β -CDEP Supported by Methylated Polystyrene for Inclusion and Adsorption of Bilirubin

    Institute of Scientific and Technical Information of China (English)

    MAO LuYuan; SHEN HanXi; ZHUANG YunFeng; YANG Yong; FAN YunGe

    2001-01-01

    @@ How to utilize functional material to eliminate uncombined bilirubin is a key problem in the research of artificial hepatic supporter [1]. There have been reports on the utilization of cyclodextrin and its polymer as biomedical materials [2,3]. In this paper, it was studied that the property of cyclodextrin polymers (MPS-CDEP) synthesized by us for the inclusion and adsorption of bilirubin. Bilirubin is the degradation product of heme (ferriprotoporohyrin IX), which body contain, and structures of both as follows:

  13. The relationship of the anti-oxidant bilirubin with free thyroxine is modified by insulin resistance in euthyroid subjects.

    Directory of Open Access Journals (Sweden)

    Petronella E Deetman

    Full Text Available BACKGROUND: The strong anti-oxidative properties of bilirubin largely explain its cardioprotective effects. Insulin resistance is featured by low circulating bilirubin. Thyroid hormone affects both bilirubin generation and its biliary transport, but it is unknown whether circulating bilirubin is associated with thyroid function in euthyroid subjects. Aim is to determine relationships of bilirubin with TSH, free T4 and free T3 in euthyroid subjects without type 2 diabetes mellitus (T2DM, and to assess whether such a relationship would be modified by the degree of insulin resistance. METHODS: Total bilirubin, TSH, free T4, free T3, glucose, insulin, lipids and transaminases were measured in 1854 fasting euthyroid subjects without T2DM, recruited from the general population (PREVEND cohort. Insulin resistance was assessed by homeostasis model assessment. RESULTS: Bilirubin was positively related to free T4 (β = 0.116, P<0.001 and free T3 (β = 0.078, P = 0.001, but bilirubin was unrelated to TSH. The relationship of bilirubin with free T4 was modified by insulin resistance with a larger effect in more insulin resistant individuals (adjusted for age and sex: β = 0.043, P = 0.056 for interaction; additionally adjusted for smoking, alcohol intake, transaminases and total cholesterol (β = 0.044, P = 0.044 for interaction. The association of bilirubin with free T4 was also modified by high density lipoprotein cholesterol (age- and sex-adjusted: β = 0.040, P = 0.072. CONCLUSIONS: Low bilirubin relates to low free T4 in euthyroid non-diabetic subjects. Low normal free T4 may particularly confer low bilirubin in more insulin resistant individuals.

  14. Bilirubin modulated cytokines, growth factors and angiogenesis to improve cutaneous wound healing process in diabetic rats.

    Science.gov (United States)

    Ram, Mahendra; Singh, Vishakha; Kumawat, Sanjay; Kant, Vinay; Tandan, Surendra Kumar; Kumar, Dinesh

    2016-01-01

    Bilirubin has shown cutaneous wound healing potential in some preliminary studies. Here we hypothesize that bilirubin facilitates wound healing in diabetic rats by modulating important healing factors/candidates and antioxidant parameters in a time-dependent manner. Diabetes was induced in male Wistar rats by streptozotocin. In all diabetic rats wounds were created under pentobarbitone anesthesia. All the rats were divided into two groups, of which one (control) was treated with ointment base and other with bilirubin ointment (0.3%). Wound closer measurement and tissue collection were done on days 3, 7, 14 and 19 post-wounding. The relative expressions of hypoxia inducible factor-1 alpha (HIF-1α), vascular endothelial growth factor (VEGF), stromal cell-derived factor-1 alpha (SDF-1α), transforming growth factor- beta1 (TGF-β1()), tumor necrosis factor-α (TNF-α) and interlukin-10 (IL-10) mRNA and proteins and the mRNA of interlukin-1 beta (IL-1β) and matrix metalloprteinase-9 (MMP-9) were determined in the wound tissues. CD-31 staining and collagen content were evaluated by immunohistochemistry and picrosirius red staining, respectively. Histopathological changes were assessed by H&E staining. The per cent wound closer was significantly higher from day 7 onwards in bilirubin-treated rats. HIF-1α, VEGF, SDF-1α, TGF-β1, IL-10 mRNA and protein levels were significantly higher on days 3, 7 and 14 in bilirubin-treated rats. The mRNA expression and protein level of TNF-α and the mRNA of IL-1β and MMP-9 were progressively and markedly reduced in bilirubin-treated rats. The collagen deposition and formation of blood vessels were greater in bilirubin-treated rats. Bilirubin markedly facilitated cutaneous wound healing in diabetic rats by modulating growth factors, cytokines, neovasculogenesis and collagen contents to the wound site. Topical application of bilirubin ointment might be of great use in cutaneous wound healing in diabetic patients.

  15. Amine-functionalized PVA-co-PE nanofibrous membrane as affinity membrane with high adsorption capacity for bilirubin.

    Science.gov (United States)

    Wang, Wenwen; Zhang, Hao; Zhang, Zhifeng; Luo, Mengying; Wang, Yuedan; Liu, Qiongzhen; Chen, Yuanli; Li, Mufang; Wang, Dong

    2017-02-01

    In this study, poly(vinyl alcohol-co-ethylene) (PVA-co-PE) nanofibrous membrane was activated by sodium hydroxide and cyanuric chloride, and then the activated membranes were functionalized by 1,3-propanediamine, hexamethylenediamine and diethylenetriamine to be affinity membranes for bilirubin removal, respectively. The chemical structures and morphologies of membranes were investigated by SEM, FTIR and XPS. And the adsorption ability of different amine-functionalized nanofibrous membranes for bilirubin was characterized. Furthermore, the effects of temperature, initial concentration of bilirubin, NaCl concentration and BSA concentration on the adsorption capacity for bilirubin of diethylenetriamine-functionalized nanofibrous membrane were studied. Results indicated that the adsorption capacity for bilirubin of diethylenetriamine-functionalized nanofibrous membrane could reach 85mg/g membrane when the initial bilirubin concentration was 200mg/L while the adsorption capacity could be increased to 110mg/g membrane if the initial bilirubin concentration was more than 400mg/L. The dynamic adsorption of diethylenetriamine-functionalized nanofibrous membrane showed that the ligands of amine groups on the membrane surface could be used as far as possible by recirculating the plasma with certain flow rates. Therefore, the diethylenetriamine-functionalized PVA-co-PE nanofibrous membrane possessed high adsorption capacity for bilirubin and it can be candidate as affinity membrane for bilirubin removal.

  16. Bilirubin degradation in methanol induced by continuous UV-B irradiation: a UHPLC--ESI-MS study.

    Science.gov (United States)

    Stanojević, J S; Zvezdanović, J B; Marković, D Z

    2015-04-01

    Degradation of bilirubin in aerobic methanol solution by continuous UV-B irradiation has been investigated in this work. The purpose of this study was to shed more light on bilirubin interaction with the UV-B component of natural sunlight, since bilirubin is a very efficient UV-B absorber located in the skin epidermis. The degradation products have been detected and studied by a combined method of Ultra High Performance Liquid Chromatography-Electrospray Ionization Mass Spectrometry (UHPLC-ESI-MS). Bilirubin, a toxic pigment which itself is a product of (hemoglobin) degradation in organisms, undergoes its own degradation under aerobic conditions of UV-B continuous irradiation (e.g. photooxidation) that can be partly self-sensitized. Two dipyrrolic structures have been identified as a result of the bilirubin degradation, not including the bilirubin derivative biliverdin whose increase in the irradiated system is synchronous with a time dynamics of bilirubin degradation. It appears that one of dipyrrolic products originates directly from bilirubin and biliverdin molecules, while the other one is probably connected to bilirubin self-sensitized degradation. The precursor role of biliverdin in the degradation process--related to the detected dipyrroles--has not been confirmed.

  17. Efficient voltammetric discrimination of free bilirubin from uric acid and ascorbic acid by a CVD nanographite-based microelectrode.

    Science.gov (United States)

    Taurino, Irene; Van Hoof, Viviane; Magrez, Arnaud; Forró, László; De Micheli, Giovanni; Carrara, Sandro

    2014-12-01

    We report a novel electrochemical sensor based on nanographite grown on platinum microelectrodes for the determination of bilirubin in the presence of normal concentrations of albumin. The albumin is a protein with an intrinsic ability to bind the bilirubin therefore reducing the concentration of the free electroactive metabolite in human fluids. In addition, the proposed device permits the discrimination of free bilirubin from two interferents, uric acid and ascorbic acid, by the separation of their oxidation peaks in voltammetry. Preliminary measurements in human serum prove that the proposed nanostructured platform can be used to detect bilirubin.

  18. Lower Serum Bilirubin and Uric Acid Concentrations in Patients with Parkinson's Disease in China.

    Science.gov (United States)

    Qin, Xiao-Ling; Zhang, Qing-Shan; Sun, Li; Hao, Meng-Wei; Hu, Zhao-Ting

    2015-05-01

    The objective of the study is to investigate the correlation between bilirubin and uric acid (UA) concentrations and symptoms of Parkinson's disease (PD) in Chinese population. A total of 425 PD patients and 460 controls were included in the current study. Patients were diagnosed by a neurologist and assessed using the Hoehn & Yahr (H&Y) scale. Venous blood samples were collected, and bilirubin and UA concentrations were analyzed. Compared to controls, indirect bilirubin (IBIL) and UA concentrations were lower in PD patients (P IBIL = 0.015, P UA = 0.000). Serum IBIL in different age subgroups and H&Y stage subgroups were also lower compared to the control group (P IBIL = 0.000, P UA = 0.000) but were not significantly different among these subgroups. Females in the control group had significantly lower serum IBIL and UA concentrations than males (P IBIL = 0.000, P UA = 0.000) and the PD group (P IBIL = 0.027, P UA = 0.000). In early PD (patients with bilirubin and UA concentrations lack the endogenous defense system to prevent peroxynitrite and other free radicals from damaging and destroying dopaminergic cells in the substantia nigra. Our results provide a basis for further investigation into the role of bilirubin in PD.

  19. Evaluation of region selective bilirubin-induced brain damage as a basis for a pharmacological treatment

    Science.gov (United States)

    Dal Ben, Matteo; Bottin, Cristina; Zanconati, Fabrizio; Tiribelli, Claudio; Gazzin, Silvia

    2017-01-01

    The neurologic manifestations of neonatal hyperbilirubinemia in the central nervous system (CNS) exhibit high variations in the severity and appearance of motor, auditory and cognitive symptoms, which is suggestive of a still unexplained selective topography of bilirubin-induced damage. By applying the organotypic brain culture (OBC: preserving in vitro the cellular complexity, connection and architecture of the in vivo brain) technique to study hyperbilirubinemia, we mapped the regional target of bilirubin-induced damage, demonstrated a multifactorial toxic action of bilirubin, and used this information to evaluate the efficacy of drugs applicable to newborns to protect the brain. OBCs from 8-day-old rat pups showed a 2–13 fold higher sensitivity to bilirubin damage than 2-day-old preparations. The hippocampus, inferior colliculus and cerebral cortex were the only brain regions affected, presenting a mixed inflammatory-oxidative mechanism. Glutamate excitotoxicity was appreciable in only the hippocampus and inferior colliculus. Single drug treatment (indomethacin, curcumin, MgCl2) significantly improved cell viability in all regions, while the combined (cocktail) administration of the three drugs almost completely prevented damage in the most affected area (hippocampus). Our data may supports an innovative (complementary to phototherapy) approach for directly protecting the newborn brain from bilirubin neurotoxicity. PMID:28102362

  20. A genome-wide association study of total bilirubin and cholelithiasis risk in sickle cell anemia.

    Directory of Open Access Journals (Sweden)

    Jacqueline N Milton

    Full Text Available Serum bilirubin levels have been associated with polymorphisms in the UGT1A1 promoter in normal populations and in patients with hemolytic anemias, including sickle cell anemia. When hemolysis occurs circulating heme increases, leading to elevated bilirubin levels and an increased incidence of cholelithiasis. We performed the first genome-wide association study (GWAS of bilirubin levels and cholelithiasis risk in a discovery cohort of 1,117 sickle cell anemia patients. We found 15 single nucleotide polymorphisms (SNPs associated with total bilirubin levels at the genome-wide significance level (p value <5 × 10(-8. SNPs in UGT1A1, UGT1A3, UGT1A6, UGT1A8 and UGT1A10, different isoforms within the UGT1A locus, were identified (most significant rs887829, p = 9.08 × 10(-25. All of these associations were validated in 4 independent sets of sickle cell anemia patients. We tested the association of the 15 SNPs with cholelithiasis in the discovery cohort and found a significant association (most significant p value 1.15 × 10(-4. These results confirm that the UGT1A region is the major regulator of bilirubin metabolism in African Americans with sickle cell anemia, similar to what is observed in other ethnicities.

  1. Transport and metabolism at blood-brain interfaces and in neural cells: relevance to bilirubin-induced encephalopathy

    Directory of Open Access Journals (Sweden)

    Silvia eGazzin

    2012-05-01

    Full Text Available Bilirubin, the end-product of heme catabolism, circulates in non pathological plasma mostly as a protein-bound species. When bilirubin concentration builds up, the free fraction of the molecule increases. Unbound bilirubin then diffuses across blood-brain interfaces into the brain, where it accumulates and exerts neurotoxic effects. In this classical view of bilirubin neurotoxicity, blood-brain interfaces act merely as structural barriers impeding the penetration of the pigment-bound carrier protein, and neural cells are considered as passive targets of its toxicity. Yet, the role of blood-brain interfaces in the occurrence of bilirubin encephalopathy appears more complex than being simple barriers to the diffusion of bilirubin, and neural cells such as astrocytes and neurons can play an active role in controlling the balance between the neuroprotective and neurotoxic effects of bilirubin. This article reviews the emerging in vivo and in vitro data showing that transport and metabolic detoxification mechanisms at the blood-brain and blood-CSF barriers may modulate bilirubin flux across both cellular interfaces, and that these protective functions can be affected in chronic hyperbilirubinemia. Then the in vivo and in vitro arguments in favor of the physiological antioxidant function of intracerebral bilirubin are presented, as well as with the potential role of transporters such as ABCC-1 and metabolizing enzymes such as cytochromes P-450 in setting the cerebral cell- and structure-specific toxicity of bilirubin following hyperbilirubinemia. The relevance of these data to the pathophysiology of bilirubin-induced neurological diseases is discussed.

  2. Sensitizing effect of Z,Z-bilirubin IXα and its photoproducts on enzymes in model solutions

    Science.gov (United States)

    Plavskii, V. Yu.; Mostovnikov, V. A.; Tret'yakova, A. I.; Mostovnikova, G. R.

    2008-05-01

    In model systems, we have studied side effects which may be induced by light during phototherapy of hyperbilirubinemia (jaundice) in newborn infants, with the aim of reducing the Z,Z-bilirubin IXα (Z,Z-BR IXα) level. We have shown that the sensitizing effect of Z,Z-BR IXα, localized at strong binding sites of the human serum albumin (HSA) macromolecule, is primarily directed at the amino acid residues of the carrier protein and does not involve the molecules of the enzyme (lactate dehydrogenase (LDH)) present in the buffer solution. The detected photodynamic damage to LDH is due to sensitization by bilirubin photoisomers, characterized by lower HSA association constants and located (in contrast to native Z,Z-BR IXα) on the surface of the HSA protein globule. Based on study of the spectral characteristics of the photoproducts of Z,Z-BR IXα and comparison of their accumulation kinetics in solution and the enzyme photo-inactivation kinetics, we concluded that the determining role in sensitized damage to LDH is played by lumirubin. The photosensitization effect depends on the wavelength of the radiation used for photoconversion of bilirubin. When (at the beginning of exposure) we make sure that identical numbers of photons are absorbed by the pigment in the different spectral ranges, the side effect is minimal for radiation corresponding to the long-wavelength edge of the bilirubin absorption band. We have shown that for a bilirubin/HSA concentration ratio >2 (when some of the pigment molecules are sorbed on the surface of the protein globule), the bilirubin can act as a photosensitizing agent for the enzyme present in solution. We discuss methods for reducing unfavorable side effects of light on the body of newborn infants during phototherapy of hyperbilirubinemia.

  3. Protein-encapsulated bilirubin: paving the way to a useful probe for singlet oxygen.

    Science.gov (United States)

    Pimenta, Frederico M; Jensen, Jan K; Etzerodt, Michael; Ogilby, Peter R

    2015-04-01

    When dissolved in a bulk solvent, bilirubin efficiently removes singlet molecular oxygen, O2(a(1)Δg), through a combination of chemical reactions and by promoting the O2(a(1)Δg)→O2(X(3)Σg(-)) nonradiative transition to populate the ground state of oxygen. To elucidate how such processes can be exploited in the development of a biologically useful fluorescent probe for O2(a(1)Δg), pertinent photophysical and photochemical parameters of bilirubin encapsulated in a protein were determined. The motivation for studying a protein-encapsulated system reflects the ultimate desire to (a) use genetic engineering to localize the probe at a specific location in a living cell, and (b) provide a controlled environment around the chromophore/fluorophore. Surprisingly, explicit values of oxygen- and O2(a(1)Δg)-dependent parameters that characterize the behavior of a given chromophore/fluorophore encased in a protein are not generally available. To the end of quantifying the effects of such an encasing protein, a recently discovered bilirubin-binding protein isolated from a Japanese eel was used. The data show that this system indeed preferentially responds to O2(a(1)Δg) and not to the superoxide ion. However, this protein not only shields bilirubin such that the rate constants for interaction with O2(a(1)Δg) decrease relative to what is observed in a bulk solvent, but the fraction of the total O2(a(1)Δg)-bilirubin interaction that results in a chemical reaction between O2(a(1)Δg) and bilirubin also decreases appreciably. The rate constants thus obtained provide a useful starting point for the general design and development of reactive protein-encased fluorescent probes for O2(a(1)Δg).

  4. Quantitative assessment of the multiple processes responsible for bilirubin homeostasis in health and disease

    Directory of Open Access Journals (Sweden)

    Levitt DG

    2014-09-01

    Full Text Available David G Levitt,1 Michael D Levitt2 1Department of Integrative Biology and Physiology, University of Minnesota, Minneapolis, MN, USA; 2Research Service, Veterans Affairs Medical Center, Minneapolis, MN, USAAbstract: Serum bilirubin measurements are commonly obtained for the evaluation of ill patients and to screen for liver disease in routine physical exams. An enormous research effort has identified the multiple mechanisms involved in the production and metabolism of conjugated (CB and unconjugated bilirubin (UB. While the qualitative effects of these mechanisms are well understood, their expected quantitative influence on serum bilirubin homeostasis has received less attention. In this review, each of the steps involved in bilirubin production, metabolism, hepatic cell uptake, and excretion is quantitatively examined. We then attempt to predict the expected effect of normal and defective function on serum UB and CB levels in health and disease states including hemolysis, extra- and intrahepatic cholestasis, hepatocellular diseases (eg, cirrhosis, hepatitis, and various congenital defects in bilirubin conjugation and secretion (eg, Gilbert's, Dubin–Johnson, Crigler–Najjar, Rotor syndromes. Novel aspects of this review include: 1 quantitative estimates of the free and total UB and CB in the plasma, hepatocyte, and bile; 2 detailed discussion of the important implications of the recently recognized role of the hepatic OATP transporters in the maintenance of CB homeostasis; 3 discussion of the differences between the standard diazo assay versus chromatographic measurement of CB and UB; 4 pharmacokinetic implications of the extremely high-affinity albumin binding of UB; 5 role of the enterohepatic circulation in physiologic jaundice of newborn and fasting hyperbilirubinemia; and 6 insights concerning the clinical interpretation of bilirubin measurements.Keywords: liver, conjugation, diazo, albumin, Rotor

  5. Regression approach to non-invasive determination of bilirubin in neonatal blood

    Science.gov (United States)

    Lysenko, S. A.; Kugeiko, M. M.

    2012-07-01

    A statistical ensemble of structural and biophysical parameters of neonatal skin was modeled based on experimental data. Diffuse scattering coefficients of the skin in the visible and infrared regions were calculated by applying a Monte-Carlo method to each realization of the ensemble. The potential accuracy of recovering the bilirubin concentration in dermis (which correlates closely with that in blood) was estimated from spatially resolved spectrometric measurements of diffuse scattering. The possibility to determine noninvasively the bilirubin concentration was shown by measurements of diffuse scattering at λ = 460, 500, and 660 nm at three source-detector separations under conditions of total variability of the skin biophysical parameters.

  6. Bilirubin diglucuronide synthesis by a UDP-glucuronic acid-dependent enzyme system in rat liver microsomes.

    Science.gov (United States)

    Blanckaert, N; Gollan, J; Schmid, R

    1979-01-01

    Incubation of rat liver homogenate or microsomal preparations with bilirubin or bilirubin monoglucuronide with (BMG) resulted in formation of bilirubin diglucuronide (BDG). Both synthesis of BMG and its conversion to BDG were critically dependent on the presence of UDP-glucuronic acid. Pretreatment of the animals with phenobarbital stimulated both reactions. When 33 microM bilirubin was incubated with microsomal preparations from phenobarbital-treated rats, 80-90% of the substrate was converted to bilirubin glucuronides; the reaction products consisted of almost equal amounts of BMG and BDG. When phenobarbital pretreatment was omitted or when the substrate concentration was increased to 164 microM bilirubin, proportionally more BMG and less BDG were formed. Homogenate and microsomes from homozygous Gunn rats neither synthesized BMG nor converted BMG to BDG. These findings in vitro suggest an explanation for the observations in vivo that, in conditions of excess bilirubin load or of genetically decreased bilirubin UDP glucuronosyltransferase (EC 2.4.1.17) activity, proportionally more BMG and less BDG are excreted in bile. PMID:109837

  7. Association between bilirubin and risk of Non-Alcoholic Fatty Liver Disease based on a prospective cohort study

    Science.gov (United States)

    Tian, Jianbo; Zhong, Rong; Liu, Cheng; Tang, Yuhan; Gong, Jing; Chang, Jiang; Lou, Jiao; Ke, Juntao; Li, Jiaoyuan; Zhang, Yi; Yang, Yang; Zhu, Ying; Gong, Yajie; Xu, Yanyan; Liu, Peiyi; Yu, Xiao; Xiao, Lin; Du, Min; Yang, Ling; Yuan, Jing; Wang, Youjie; Chen, Weihong; Wei, Sheng; Liang, Yuan; Zhang, Xiaomin; He, Meian; Wu, Tangchun; Yao, Ping; Miao, Xiaoping

    2016-01-01

    The study aimed to assess the association between total, direct, and indirect bilirubin and nonalcoholic fatty live disease (NAFLD) risk given its high prevalence and serious clinical prognosis. Among 27,009 subjects who participated in a healthy screening program from the Dongfeng-Tongji cohort study in 2008, 8189 eligible subjects (aged 35–86 years; males, 43.95%) were ultimately enrolled. The incidence rates of NAFLD in 2013 were compared with respect to baseline bilirubin levels among subjects free of NAFLD, and the effect sizes were estimated by logistic regression analysis. During 5 years follow-up, we observed 1956 cases of newly developed NAFLD with the overall incidence of 23.88%. Direct bilirubin was presented to inversely associate with NAFLD risk. Compared with quartile 1 of direct bilirubin, the multivariable-adjusted ORs (95% CIs) for NAFLD of quartile 2 to 4 were 1.104 (0.867–1.187), 0.843 (0.719–0.989), and 0.768 (0.652–0.905), respectively, P for trend 0.002). Similarly, inverse effects of direct bilirubin on NAFLD incidence were also observed when stratified by sex and BMI. However, no significant associations were found between total, and indirect bilirubin and NAFLD risk. Direct bilirubin reduced NAFLD risk independent of possible confounders among middle-aged and elderly Chinese population, probably based on the endogenous antioxidation of bilirubin. PMID:27484402

  8. Evaluation of the Predictive Value of Umbilical Cord Serum Bilirubin Level for the Development of Subsequent Hyperbilirubinemia in Term and Late-Preterm Neonates

    Directory of Open Access Journals (Sweden)

    Fariba Hemmati

    2016-03-01

    Conclusion: Based on the findings, UCS bilirubin level could not predict subsequent hyperbilirubinemia. Therefore, the best predictive marker for neonatal jaundice is the assessment of clinical risk factors and predischarge bilirubin level.

  9. Microchip capillary electrophoresis for frontal analysis of free bilirubin and study of its interaction with human serum albumin.

    Science.gov (United States)

    Nie, Zhou; Fung, Ying Sing

    2008-05-01

    To meet the need for bedside monitoring of free bilirubin for neonates under critical conditions, a microfluidic chip was fabricated and tested for its coupling with CE/frontal analysis (FA) to determine free bilirubin and study of its binding interaction with HSA, which regulated its concentration in plasma. The poly(methyl methacrylate) (PMMA) multichannel chip was fabricated by CO2 laser ablation and bonded with a fused-silica separation capillary for CE/FA separation with UV detection. The chip was designed to allow a complete assay of four electrophoretic runs using preconditioned channels to speed up the determination of free bilirubin and to deliver quick results for bedside monitoring. Under optimized conditions, the linear working range for free bilirubin was from 10 to 200 micromol with RSDs from 2.1 to 5.0% for n=3, and the LOD at 9 micromol for S/N=3. From a binding study between bilirubin and HSA under FA condition, the second binding constant for bilirubin-HSA was determined as 1.07x10(5) L/mol and the number of binding sites per HSA as 3.46. The results enabled the calculation of free bilirubin for jaundiced infants based on the clinically significant level of total bilirubin, producing a range of 118.3-119.4 micromol/L. The developed method is shown to meet the clinical requirement with additional margin of protection to detect the early rising level of free bilirubin prior to jaundice condition. The low-cost microchip CE/FA device is shown to produce quick results with high potential to deliver a suitable bed-side monitoring method for bilirubin management in neonates.

  10. Circular dichroism study of the interaction between mutagens and bilirubin bound to different binding sites of serum albumins

    Science.gov (United States)

    Orlov, Sergey; Goncharova, Iryna; Urbanová, Marie

    Although recent investigations have shown that bilirubin not only has a negative role in the organism but also exhibits significant antimutagenic properties, the mechanisms of interactions between bilirubin and mutagens are not clear. In this study, interaction between bilirubin bound to different binding sites of mammalian serum albumins with structural analogues of the mutagens 2-aminofluorene, 2,7-diaminofluorene and mutagen 2,4,7-trinitrofluorenone were investigated by circular dichroism and absorption spectroscopy. Homological human and bovine serum albumins were used as chiral matrices, which preferentially bind different conformers of bilirubin in the primary binding sites and make it observable by circular dichroism. These molecular systems approximated a real system for the study of mutagens in blood serum. Differences between the interaction of bilirubin bound to primary and to secondary binding sites of serum albumins with mutagens were shown. For bilirubin bound to secondary binding sites with low affinity, partial displacement and the formation of self-associates were observed in all studied mutagens. The associates of bilirubin bound to primary binding sites of serum albumins are formed with 2-aminofluorene and 2,4,7-trinitrofluorenone. It was proposed that 2,7-diaminofluorene does not interact with bilirubin bound to primary sites of human and bovine serum albumins due to the spatial hindrance of the albumins binding domains. The spatial arrangement of the bilirubin bound to serum albumin along with the studied mutagens was modelled using ligand docking, which revealed a possibility of an arrangement of the both bilirubin and 2-aminofluorene and 2,4,7-trinitrofluorenone in the primary binding site of human serum albumin.

  11. Preparation and adsorption property of aminated cross linking microbeads of GMA/EGDMA for bilirubin

    Indian Academy of Sciences (India)

    Zhiping Chen; Baojiao Gao; Xiaofeng Yang

    2009-11-01

    Cross linking microbeads with a controllable diameter were synthesized by suspension copolymerization of the monomer glycidyl methacrylate (GMA) and the cross linking agent ethylene glycol dimethylacrylate (EGDMA). By the ring-opening reaction of the epoxy groups, the microbeads GMA/EGDMA were modified with different aminating agents and resulting in the aminated microbeads. The morphology of the microbeads was characterized by SEM. The adsorption property of aminated microbeads for bilirubin was investigated, and the effects of various factors, such as the chemical structures of the aminating agents, pH values of the medium and the presence of bovine serum albumin in the adsorption medium, on the adsorption property were examined. The experimental results show that the aminated microbeads have strong adsorption ability for bilirubin, and the isotherm adsorption behaviour is fitted to Freundlich equation satisfactorily. The adsorption ability of the aminated microbeads modified with hexanediamine is stronger than that of others, and the longer the molecule of multi-ethylene multiamine, the weaker the adsorption ability for bilirubin. The pH value of the medium affects the adsorption ability greatly, as pH = 6, the adsorption ability is strongest. In the presence of BSA, the microbeads still have a higher adsorption capacity towards bilirubin.

  12. Deracemization of bilirubin as the marker of the chirality of micellar aggregates.

    Science.gov (United States)

    Sorrenti, Alessandro; Altieri, Barbara; Ceccacci, Francesca; Di Profio, Pietro; Germani, Raimondo; Giansanti, Luisa; Savelli, Gianfranco; Mancini, Giovanna

    2012-01-01

    The deracemization of bilirubin in micellar aggregates of structurally correlated chiral surfactants was studied by circular dichroism experiments and exploited as the marker of the expression of chirality of the aggregates. The obtained results suggest that the hydrophobic interactions control the transfer of chirality from the monomers to the aggregates, and that different regions of the same aggregate might feature opposite enantiorecognition capabilities.

  13. Functionalized Magnetic Fe3O4-β-Cyclodextran Nanoparticles for Efficient Removal of Bilirubin.

    Science.gov (United States)

    Han, Lulu; Chu, Simin; Wei, Houliang; Ren, Jun; Xu, Li; Jia, Lingyun

    2016-06-01

    Bilirubin (BR), as a lipophilic toxin, can binds and deposits in various tissues, especially the brain tissue, leading to hepatic coma and even death. Magnetic nanoparticles adsorbent modified by β-cyclodextran (Fe3O4-β-CD) was developed to remove the BR from the plasma. Fe3O4-β-CD nanoparticles was prepared through Schiff base reaction between the polyethylenimine (PEI)-modified Fe3O4 and aldehyde-functionalized β-CD, and characterized by Fourier transform infrared (FTIR) spectra, X-ray diffraction (XRD), transmission electron microscopy (TEM), vibrating sample magnetometer (VSM) and dynamic light scattering (DLS). Under optimized conditions, the Fe3O4-β-CD adsorbent could adsorb 225.6 mg/g free BR in PBS and reach the adsorption equilibrium within 90 min mainly through hydrophobic interaction; Moreover, the adsorbent displayed better adsorption capability in a dialysis system for BSA-bound bilirubin, plasma bilirubin and total bile acid, and the removal rates of those were 66%, 31% and 41% respectively. Because of the advantages of fast separation and purification process, low preparation cost, good adsorption capability for plasma bilirubin, Fe3O4-β-CD may become an economical and promising absorbent of BR for clinical applications.

  14. Kinetics of oxidation of bilirubin and its protein complex by hydrogen peroxide in aqueous solutions

    Science.gov (United States)

    Solomonov, A. V.; Rumyantsev, E. V.; Antina, E. V.

    2010-12-01

    A comparative study of oxidation reactions of bilirubin and its complex with albumin was carried out in aqueous solutions under the action of hydrogen peroxide and molecular oxygen at different pH values. Free radical oxidation of the pigment in both free and bound forms at pH 7.4 was shown not to lead to the formation of biliverdin, but to be associated with the decomposition of the tetrapyrrole chromophore into monopyrrolic products. The effective and true rate constants of the reactions under study were determined. It was assumed that one possible mechanism of the oxidation reaction is associated with the interaction of peroxyl radicals and protons of the NH groups of bilirubin molecules at the limiting stage with the formation of a highly reactive radical intermediate. The binding of bilirubin with albumin was found to result in a considerable reduction in the rate of the oxidation reaction associated with the kinetic manifestation of the protein protection effect. It was found that the autoxidation of bilirubin by molecular oxygen with the formation of biliverdin at the intermediate stage can be observed with an increase in the pH of solutions.

  15. Prognostic value of serum total bilirubin in patients with acute coronary syndrome after percutaneous coronary intervention

    Institute of Scientific and Technical Information of China (English)

    孙同文

    2013-01-01

    Objective To investigate the predictive value of serum total bilirubin (STB) level in patients with acute coronary syndrome (ACS) after percutaneous coronary intervention (PCI) .Methods A total of 1273 consecutive patients treated with PCI in cardiology department,First Affiliated Hospital of Zhengzhou University from June

  16. Surface modifying of microporous PTFE capillary for bilirubin removing from human plasma and its blood compatibility

    Energy Technology Data Exchange (ETDEWEB)

    Jin Gu [Department of Chemistry, University of Science and Technology of China, HeFei, 230026 (China)], E-mail: Gjin@ustc.edu.cn; Yao Qizhi; Zhang Shanzi [Department of Chemistry, University of Science and Technology of China, HeFei, 230026 (China); Zhang Lei [Department of Chemistry, University of Science and Technology of China, HeFei, 230026 (China); AnHui Entry Exit Inspection and Quarantine Bureau, HeFei, 230001 (China)

    2008-12-01

    In this study, human serum albumin (HSA) was covalently immobilized onto the inner surface of microporous poly(tetrafluoroethylene) (MPTFE) capillaries for direct bilirubin removal from human plasma. To obtain active binding sites for HSA, the MPTFE capillaries were chemically functionalized by using a coating of poly(vinyl alcohol) (PVA)-glycidyl methacrylate (GMA) copolymers. Characterization of grafted MPTFE capillaries was verified by XPS, Fourier transform infrared spectroscopy (FT-IR), scanning electronic microscopy (SEM). Non-specific adsorption on the PVA-GMA coated capillary remains low (< 0.38 mg bilirubin/g), and higher affinity adsorption capacity, of up to 73.6 mg bilirubin/g polymer was obtained after HSA is immobilized. Blood compatibility of the grafted MPTFE capillary was evaluated by SEM and platelet rich plasma (PRP) contacting experiments. The experimental data on blood compatibility indicated that PVA-coated and PVA-GMA-HSA coated PTFE capillary showed a sharp suppress on platelets adhesion. The proposed method has the potential of serving in bilirubin removal in clinical application.

  17. EKTACHEM bilirubin fraction Bc as a predictor of liver transplant rejection.

    Science.gov (United States)

    Cox, C J; Valdiserri, R O; Zerbe, T R; Genter, J L

    1987-10-01

    Bilirubin fractions Bc and DELTA, not routinely available prior to the EKTACHEM Chemistry Analyzer and its slide methodology, were studied in an outpatient population of liver transplant recipients. A preliminary evaluation by the authors has shown that direct bilirubin (DBILI) levels in the normal range consist almost exclusively of DELTA (protein-bound conjugated bilirubin), while at elevated DBILI levels, an increasing amount of Bc (non-protein-bound conjugated bilirubin) is measured as well. The present study evaluated the clinical significance of Bc in the serum of 80 liver transplant recipients as a means of identifying episodes of rejection. Each patient was classified into rejection or nonrejection categories based on clinical status, liver biopsy results, and/or response to therapy. Eighteen patients were classified as experiencing an episode of rejection during the period of this study. Fourteen of these (77.8%) had Bc levels that ranged from 0.1 to 6.8 mg/dl. Sixty two patients were classified in the nonrejection category. Fourteen (22.6%) of these patients had Bc levels that ranged from 0.1 to 0.6 mg/dl. In our outpatient liver transplant recipients with Bc greater than or equal to 0.1 mg/dl, the relative risk of rejection (% of rejection patients with Bc/% of nonrejection patients with Bc) was 3.44. This value indicates that Bc determination may be a helpful adjunct in the assessment of rejection.

  18. 21 CFR 862.1110 - Bilirubin (total or direct) test system.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Bilirubin (total or direct) test system. 862.1110 Section 862.1110 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICAL DEVICES CLINICAL CHEMISTRY AND CLINICAL TOXICOLOGY DEVICES Clinical Chemistry...

  19. A pro-inflammatory glycoprotein biomarker is associated with lower bilirubin in metabolic syndrome

    NARCIS (Netherlands)

    Dullaart, Robin P. F.; Gruppen, Eke G.; Connelly, Margery A.; Lefrandt, Joop D.

    2015-01-01

    Objectives: Bilirubin exerts anti-oxidative and anti-inflammatory properties which may beneficially influence the development of cardio-metabolic disorders. A nuclear magnetic resonance (NMR) spectroscopy-based glycoprotein biomarker, designated GlycA, whose signal originates from several glycosylat

  20. Assessment of duodenogastric reflux by combined continuous intragastric pH and bilirubin monitoring

    Institute of Scientific and Technical Information of China (English)

    Fei Dai; Jun Gong; Ru Zhang; Jin-Yan Luo; You-Ling Zhu; Xue-Qin Wang

    2002-01-01

    AIM: To assess the diagnostic value of a combination of continuous intragastric pH and bilirubin monitoring in the detection of duodenogastric reflux (DGR), and the effects of diet on the bilirubin absorbance.METHODS: 30 healthy volunteers were divided into twogroups: standard diet group (Group 1) 18 cases, free diet group (Group 2) 12 cases. Each subjects were subjected to simultaneous 24 hour intragastric pH and spectrophotometric bilirubin concentration monitoring (Bilitec 2000).RESULTS: There was no difference of preprandial phasebilirubin absorbance between two groups. The absorbanceof postprandial phase was significantly increased in group 2than group 1. There was no difference between preprandialphase and postprandial phase absorbance in group 1.Postprandial phase absorbance was significantly higher ingroup 2. In a comparison of bile reflux with intragastric pHduring night time, there were 4 types of reflux:Simultaneous increase in absorbance and pH in only 19.6%, increase in bilirubin with unchanged pH 33. 3 %, pHincrease with unchanged absorbance 36. 3 %, and bothunchanged in 10. 8 %. Linear regression analysis showed nocorrelation between percertage total time of pH < 4 aridpercentage total time of absortance > 0. 14, r=0.068, P<0.05.CONCLUSION: Because of the dietary effect, highabsorbance fluids or foods should be avoided in detection.Intrsgastric pH and bilirubin monitoring separately predictthe presence of duodenal (and/or pancreatic) reflux and bilereflux. They can not substitute for each other. The detectionof DGR is improved if the two parameters are combinedsimultanoously.

  1. Selective nonenzymatic bilirubin detection in blood samples using a Nafion/Mn-Cu sensor.

    Science.gov (United States)

    Noh, Hui-Bog; Won, Mi-Sook; Shim, Yoon-Bo

    2014-11-15

    The specific detection of biological organics without the use of an enzyme is challenging, and it is crucial for analytical and clinical chemistry. We report specific nonenzymatic bilirubin detection through the catalytic oxidation of bilirubin molecule on the Nafion/Mn-Cu surface. The catalytic ability, true surface area, morphology, crystallinity, composition, and oxidation state of the sensor surface were assessed using voltammetry, coulometry, XPS, XRD, Brunauer-Emmett-Teller (BET), SEM, EDXS, and TOF-SIMS experiments. The results showed that the surface was composed of microporous Mn-Cu bimetallic crystal in flake shape with a large BET surface area (3.635 m(2)g(-1)), where the surface area and crystallinity mainly affected the sensor performance. Product analysis of the catalytic reaction on the sensor probe revealed a specific two-electron oxidation of dipyrromethane moiety to dipyrromethene in the bilirubin molecule. Experimental variables affecting the analysis of bilirubin were optimized in terms of probe composition, temperature, pH, and potential. At the optimized condition, the dynamic range was between 1.2 μM and 0.42 mM, which yielded the equation of ΔI (μA)=(1.03 ± 0.72)+(457.0 ± 4.03) [C] (mM) with 0.999 of correlation coefficient, and the detection limit was 25.0 ± 1.8 nM (n=5, k=3). The stability test, interference effects, and analysis of real clinical samples, human whole blood and certified serum samples were demonstrated to confirm the reliability of the proposed bilirubin sensor.

  2. Three-dimensionally porous graphene: A high-performance adsorbent for removal of albumin-bonded bilirubin.

    Science.gov (United States)

    Ma, Chun Fang; Gao, Qiang; Xia, Kai Sheng; Huang, Zhi Yuan; Han, Bo; Zhou, Cheng Gang

    2017-01-01

    The development of bilirubin adsorbents with high adsorption efficiencies towards albumin-bonded bilirubin is still a considerable challenge. In this work, a three-dimensionally porous graphene (3D-pGR) has been fabricated through a simple carbon dioxide (CO2) activation of thermally exfoliated graphite oxide (EGO). Intriguingly, the resultant 3D-pGR material showed hierarchically micro-meso-macroporous structure, high specific surface area of up to 843m(2)g(-1), and large pore volume as high as 2.71cm(3)g(-1). Besides, the large planar π-configuration structure of 3D-pGR made it possible to compete effectively with albumin for bilirubin binding. Taking advantages of these fantastic characteristics, the 3D-pGR was demonstrated to be extraordinarily efficient for bilirubin removal from a bovine serum albumin (BSA)-rich solution. Under optimized conditions, the maximum adsorption capacity of 3D-pGR for BSA-bonded bilirubin was up to 126.1mgg(-1), which is not only significantly higher than the adsorption capacities of currently available adsorbents towards albumin-bonded bilirubin, but also superior to those of many reported adsorbents towards free bilirubin. In addition, the hemolysis assay of 3D-pGR indicated that this material had negligible hemolysis effect. Findings from this study may open up important new possibilities for removal of protein-bonded toxins.

  3. BILIRUBIN CONCENTRATIONS IN CLINICALLY HEALTHY AND DISEASED CAPTIVE WATERBUCK (KOBUS ELLIPSIPRYMNUS) AT THE SAN DIEGO ZOO SAFARI PARK.

    Science.gov (United States)

    Sadler, Ryan A; Lamberski, Nadine; Christopher, Mary M

    2016-06-01

    Captive waterbuck ( Kobus ellipsiprymnus ) that appear clinically healthy have been noted to have high serum bilirubin concentrations compared with other ruminants; however, questions remain about the physiologic factors affecting bilirubin concentration and its potential association with underlying disease and icteric serum or mucous membranes. Serum bilirubin concentrations of healthy and diseased waterbuck housed at the San Diego Zoo Safari Park from 1989 to 2012 were retrospectively analyzed to determine any link between icteric serum, total bilirubin concentration (tBili), and disease entities in this species. Total bilirubin and direct (dBili) bilirubin concentrations and the prevalence of icteric serum were compared by subspecies, age group, and health status; associations with complete blood count and biochemical results and clinical diagnosis were assessed. No significant differences were found in tBili or dBili between Ellipsen (n = 32) and Defassa (n = 29) subspecies or in juveniles (n = 22) versus adults (n = 39). Clinically healthy waterbuck (n = 40) had significantly higher tBili (mean ± 2SD, 7.9 ± 1.2 mg/dl; P bilirubin (2.2-6.2 mg/dl). These results suggest healthy waterbuck have relatively high tBili and dBili compared with related species. Icteric serum may be seen in up to 15% of healthy animals in the absence of icteric tissues.

  4. Looking to the horizon: the role of bilirubin in the development and prevention of age-related chronic diseases.

    Science.gov (United States)

    Wagner, Karl-Heinz; Wallner, Marlies; Mölzer, Christine; Gazzin, Silvia; Bulmer, Andrew Cameron; Tiribelli, Claudio; Vitek, Libor

    2015-07-01

    Bilirubin, the principal tetrapyrrole, bile pigment and catabolite of haem, is an emerging biomarker of disease resistance, which may be related to several recently documented biological functions. Initially believed to be toxic in infants, the perception of bilirubin has undergone a transformation: it is now considered to be a molecule that may promote health in adults. Data from the last decade demonstrate that mildly elevated serum bilirubin levels are strongly associated with reduced prevalence of chronic diseases, particularly cardiovascular diseases (CVDs), as well as CVD-related mortality and risk factors. Recent data also link bilirubin to other chronic diseases, including cancer and Type 2 diabetes mellitus, and to all-cause mortality. Therefore, there is evidence to suggest that bilirubin is a biomarker for reduced chronic disease prevalence and a predictor of all-cause mortality, which is of important clinical significance. In the present review, detailed information on the association between bilirubin and all-cause mortality, as well as the pathological conditions of CVD, cancer, diabetes and neurodegenerative diseases, is provided. The mechanistic background concerning how bilirubin and its metabolism may influence disease prevention and its clinical relevance is also discussed. Given that the search for novel biomarkers of these diseases, as well as for novel therapeutic modalities, is a key research objective for the near future, bilirubin represents a promising candidate, meeting the criteria of a biomarker, and should be considered more carefully in clinical practice as a molecule that might provide insights into disease resistance. Clearly, however, greater molecular insight is warranted to support and strengthen the conclusion that bilirubin can prevent disease, with future research directions also proposed.

  5. Bilirubin activates transcription of HIF-1α in human proximal tubular cells cultured in the physiologic oxygen content.

    Science.gov (United States)

    Kim, Sung Gyun; Ahn, Shin-Young; Lee, Eun Seong; Kim, Sejoong; Na, Ki Young; Chae, Dong-Wan; Chin, Ho Jun

    2014-09-01

    The expression of hypoxia-inducible factor (HIF) is influenced by reactive oxygen species (ROS). Effect of bilirubin on HIF-1 expression in proximal tubular cells was investigated under physiological oxygen concentration, which is relative hypoxic condition mimicking oxygen content in the medulla of renal tissue. The human kidney (HK2) cells were cultured in 5% oxygen with or without bilirubin. HIF-1α protein expression was increased by bilirubin treatment at 0.01-0.2 mg/dL concentration. The messenger RNA expression of HIF-1α was increased by 1.69±0.05 folds in the cells cultured with 0.1 mg/dL bilirubin, compared to the control cells. The inhibitors of PI3K/mTOR, PI3K/AKT, and ERK 1/2 pathways did not attenuate increased HIF-1α expression by bilirubin. HIF-1α expression decreased by 10 µM exogenous hydrogen peroxide (H2O2); scavenger of ROS with or without bilirubin in the HK2 cells increased HIF-1α concentration more than that in the cells without bilirubin. Exogenous H2O2 decreased the phosphorylation of P70S6 kinase, which was completely reversed by bilirubin treatment. Knockdown of NOX4 gene by small interfering RNA (siRNA) increased HIF-1α mRNA expression. In coonclusion, bilirubin enhances HIF-1α transcription as well as the up-regulation of HIF-1α protein translation through the attenuation of ROS and subunits of NADPH oxidase.

  6. Bilirubin prevents acute DSS-induced colitis by inhibiting leukocyte infiltration and suppressing upregulation of inducible nitric oxide synthase.

    Science.gov (United States)

    Zucker, Stephen D; Vogel, Megan E; Kindel, Tammy L; Smith, Darcey L H; Idelman, Gila; Avissar, Uri; Kakarlapudi, Ganesh; Masnovi, Michelle E

    2015-11-15

    Bilirubin is thought to exert anti-inflammatory effects by inhibiting vascular cell adhesion molecule-1 (VCAM-1)-dependent leukocyte migration and by suppressing the expression of inducible nitric oxide synthase (iNOS). As VCAM-1 and iNOS are important mediators of tissue injury in the dextran sodium sulfate (DSS) murine model of inflammatory colitis, we examined whether bilirubin prevents colonic injury in DSS-treated mice. Male C57BL/6 mice were administered 2.5% DSS in the drinking water for 7 days, while simultaneously receiving intraperitoneal injections of bilirubin (30 mg/kg) or potassium phosphate vehicle. Disease activity was monitored, peripheral blood counts and serum nitrate levels were determined, and intestinal specimens were analyzed for histological injury, leukocyte infiltration, and iNOS expression. The effect of bilirubin on IL-5 production by HSB-2 cells and on Jurkat cell transendothelial migration also was determined. DSS-treated mice that simultaneously received bilirubin lost less body weight, had lower serum nitrate levels, and exhibited reduced disease severity than vehicle-treated animals. Concordantly, histopathological analyses revealed that bilirubin-treated mice manifested significantly less colonic injury, including reduced infiltration of eosinophils, lymphocytes, and monocytes, and diminished iNOS expression. Bilirubin administration also was associated with decreased eosinophil and monocyte infiltration into the small intestine, with a corresponding increase in peripheral blood eosinophilia. Bilirubin prevented Jurkat migration but did not alter IL-5 production. In conclusion, bilirubin prevents DSS-induced colitis by inhibiting the migration of leukocytes across the vascular endothelium and by suppressing iNOS expression.

  7. Gene therapy with bilirubin-UDP-glucuronosyltransferase in the Gunn rat model of Crigler-Najjar syndrome type 1.

    Science.gov (United States)

    Li, Q; Murphree, S S; Willer, S S; Bolli, R; French, B A

    1998-03-01

    Crigler-Najjar syndrome type 1 (CN type 1) is an autosomal recessive disorder characterized by nonhemolytic jaundice resulting from mutations to the gene encoding bilirubin-UDP-glucuronosyltransferase (UDPGT). The Gunn rat is an accurate animal model of this disease because the bilirubin-UDPGT gene in this strain carries a premature stop codon. The primary objective of this study was to complement this deficiency in vivo using liver-directed gene therapy. The efficiency of adenovirus type 5 (Ad5)-mediated gene transfer to the neonatal rat liver was first assessed by intravenous (i.v.) injection of an Ad5 vector carrying a nuclear-localized LacZ gene. An Ad5 vector expressing the cDNA encoding human bilirubin-UDPGT (Ad5/CMV/hUG-Br1) was then generated and injected i.v. into neonatal Gunn rats. Plasma samples were collected and bilirubin levels were determined at regular intervals. Although the mean level of bilirubin in homozygous Gunn rats 1-2 days after birth was already 14.5-fold higher than that of heterozygous siblings, treatment with Ad5/CMV/hUG-Br1 reduced plasma bilirubin to normal levels within 1 week. Plasma bilirubin in the treated homozygous rats remained normal for 4 weeks before gradually climbing to intermediate levels that were approximately half that of untreated homozygotes by 12 weeks. Administration of Ad5-mediated gene therapy to neonatal Gunn rats effectively complemented the deficiency in bilirubin-UDPGT, resulting in substantial reductions in plasma bilirubin over a 3-month period. The efficacy of Ad5-mediated gene therapy in neonates suggests that this approach might be effective against other hepatic disorders, including autosomal recessive deficiencies in lipid metabolism and vascular homeostasis.

  8. Mildly elevated serum bilirubin levels are negatively associated with carotid atherosclerosis among elderly persons.

    Directory of Open Access Journals (Sweden)

    Ryuichi Kawamoto

    Full Text Available Serum bilirubin may have a beneficial role in preventing oxidative changes in atherosclerosis. Limited information is available on whether serum total bilirubin is an independent confounding factor for carotid atherosclerosis {for example, intima-media thickness (IMT, plaque} measured noninvasively by B-mode ultrasonography only among elderly persons. The study subjects were 325 men aged 79 ± 8 (mean ± standard deviation years and 509 women aged 81 ± 8 years that were enrolled consecutively from patients aged ≥ 60 years in the medical department. Carotid IMT and plaque were derived via B-mode ultrasonography. Multiple linear regression analysis showed that in men age (β = 0.199, p = 0.002, smoking status (β = 0.154, p = 0.006, GGT (β = -0.139, p = 0.039, and GGT (β = -0.133, p = 0.022 were significantly and independently associated with carotid IMT, and in women age (β = 0.186, p < 0.001, systolic blood pressure (β = 0.104, p = 0.046, diastolic blood pressure (β = -0.148, p = 0.004, prevalence of antihypertensive medication (β = 0.126, p = 0.004, fasting plasma glucose (β = 0.135, p = 0.003, GGT (β = -0.104, p = 0.032, estimated glomerular filtration rate, serum bilirubin (β = -0.119, p = 0.006, and prevalence of cardiovascular disease (CVD (β = 0.103, p = 0.017 were also independently associated with carotid IMT. The odds ratios (ORs {95% confidence interval (CI} of increasing serum bilirubin category were negatively associated with carotid IMT ≥ 1.0 mm and plaque in both genders. Compared to subjects with a serum bilirubin of Quartile-1, the multivariate-OR (95% CI of carotid plaque was 0.25 (0.11-0.57 in the Quartile-4 male group, and 0.41 (0.21-0.78 in the Quartile-2 female group, 0.51 (0.26-0.98 in the Quartile-3 female group, and 0.46 (0.24-0.89 in the Quartile-4 female group. Our data demonstrated an independently negative association between serum bilirubin and carotid atherosclerosis in both genders.

  9. Inducible bilirubin oxidase: A novel function for the mouse cytochrome P450 2A5

    Energy Technology Data Exchange (ETDEWEB)

    Abu-Bakar, A' edah, E-mail: a.abubakar@uq.edu.au [The University of Queensland, National Research Centre for Environmental Toxicology (Entox), 4072 Brisbane, Queensland (Australia); Arthur, Dionne Maioha [The University of Queensland, National Research Centre for Environmental Toxicology (Entox), 4072 Brisbane, Queensland (Australia); Cooperative Research Centre for Contamination Assessment and Remediation of the Environment, Adelaide (Australia); Aganovic, Simona [Department of Pharmaceutical Biosciences, Uppsala University, Biomedical Centre, Box 578, S-751 23 Uppsala (Sweden); Ng, Jack C. [The University of Queensland, National Research Centre for Environmental Toxicology (Entox), 4072 Brisbane, Queensland (Australia); Cooperative Research Centre for Contamination Assessment and Remediation of the Environment, Adelaide (Australia); Lang, Matti A. [The University of Queensland, National Research Centre for Environmental Toxicology (Entox), 4072 Brisbane, Queensland (Australia); Department of Pharmaceutical Biosciences, Uppsala University, Biomedical Centre, Box 578, S-751 23 Uppsala (Sweden)

    2011-11-15

    We have previously shown that bilirubin (BR), a breakdown product of haem, is a strong inhibitor and a high affinity substrate of the mouse cytochrome P450 2A5 (CYP2A5). The antioxidant BR, which is cytotoxic at high concentrations, is potentially useful in cellular protection against oxygen radicals if its intracellular levels can be strictly controlled. The mechanisms that regulate cellular BR levels are still obscure. In this paper we provide preliminary evidence for a novel function of CYP2A5 as hepatic 'BR oxidase'. A high-performance liquid chromatography/electrospray ionisation mass spectrometry screening showed that recombinant yeast microsomes expressing the CYP2A5 oxidise BR to biliverdin, as the main metabolite, and to three other smaller products with m/z values of 301, 315 and 333. The metabolic profile is significantly different from that of chemical oxidation of BR. In chemical oxidation the smaller products were the main metabolites. This suggests that the enzymatic reaction is selective, towards biliverdin production. Bilirubin treatment of primary hepatocytes increased the CYP2A5 protein and activity levels with no effect on the corresponding mRNA. Co-treatment with cycloheximide (CHX), a protein synthesis inhibitor, resulted in increased half-life of the CYP2A5 compared to cells treated only with CHX. Collectively, the observations suggest that the CYP2A5 is potentially an inducible 'BR oxidase' where BR may accelerate its own metabolism through stabilization of the CYP2A5 protein. It is possible that this metabolic pathway is potentially part of the machinery controlling intracellular BR levels in transient oxidative stress situations, in which high amounts of BR are produced. -- Highlights: Black-Right-Pointing-Pointer CYP2A5 metabolizes bilirubin to biliverdin and dipyrroles. Black-Right-Pointing-Pointer Bilirubin increased the hepatic CYP2A5 protein and activity levels. Black-Right-Pointing-Pointer Bilirubin does not

  10. 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) increases bilirubin formation but hampers quantitative hepatic conversion of biliverdin to bilirubin in rats with wild-type AH receptor.

    Science.gov (United States)

    Niittynen, Marjo; Simanainen, Ulla; Pohjanvirta, Raimo; Sankari, Satu; Tuomisto, Jouni T

    2014-06-01

    In haem degradation, haem oxygenase-1 (HO-1) first cleaves haem to biliverdin, which is reduced to bilirubin by biliverdin IXα reductase (BVR-A). The environmental pollutant 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) causes hepatic accumulation of biliverdin in moderately TCDD-resistant line B (Kuopio) rats. Using line B and two TCDD-sensitive rat strains, the present study set out to probe the dose-response and biochemical mechanisms of this accumulation. At 28 days after exposure to 3-300 μg/kg TCDD in line B rats, already the lowest dose of TCDD tested, 3 μg/kg, affected serum bilirubin conjugates, and after doses ≥100 μg/kg, the liver content of bilirubin, biliverdin and their conjugates (collectively 'bile pigments') as well as HO-1 was elevated. BVR-A activity and serum bile acids were increased only by the doses of 100 and 300 μg/kg TCDD, respectively. Biliverdin conjugates correlated best with biliverdin suggesting it to be their immediate precursor. TCDD (100 μg/kg, 10 days) increased hepatic bilirubin and biliverdin levels also in TCDD-sensitive Long-Evans (Turku/AB; L-E) rats. Hepatic bilirubin and bile acids, but not biliverdin, were increased in feed-restricted L-E control rats. In TCDD-sensitive line C (Kuopio) rats, 10 μg/kg of TCDD increased the body-weight-normalized biliary excretion of bilirubin. Altogether, the results suggest that at acutely toxic doses, TCDD induces the formation of bilirubin in rats. However, concurrently, TCDD seems to hamper the quantitative conversion of biliverdin to bilirubin in line B and L-E rats' liver. Biliverdin conjugates are most likely formed as secondary products of biliverdin.

  11. THE ROLE OF DIETARY PROPOLIS ON ALBUMINS AND BILIRUBIN CONTENT IN CHICKENS

    Directory of Open Access Journals (Sweden)

    Marcela Capcarová

    2013-12-01

    Full Text Available The present study was designed to determinate the effect of propolis as a feed additive on the serum bilirubin and albumin content of female and male chickens. Broiler chickens hybrid Hubbard JV (n=500 were divided into five groups in each gender (control – C and four experimental groups E1 – E4. Experimental chickens received a propolis extract in feed mixture in various doses (E1 – 150 mg/kg; E2 – 450 mg/kg; E3 – 600 mg/kg; E4 – 800 mg/kg. The group that received feed without propolis addition served as the control. Contents of albumin and bilirubin were determined with spectrophotometer. Supplementation of the diet with propolis in the dose of 600 mg/kg significantly (P<0.05 increased albumin content in male chickens. Propolis addition to diets may be a source for antioxidant capacity in human and animals.

  12. Increased conjugated bilirubin is sufficient to initiate screening for biliary atresia

    DEFF Research Database (Denmark)

    Madsen, Stine Skipper; Kvist, Nina; Thorup, Jørgen

    2015-01-01

    . This percentage value has caused diagnostic trouble over the years. The objective of the present study was to investigate the possibility of changing the recommendations. METHODS: This was a retrospective analysis of the medical records of children operated for biliary atresia in the 1993-2012 period. RESULTS......: During the period, 73 patients where operated with a portoenterostomy ad modum Kasai. Patients older than 84 days at the time of operation were excluded, 54 patients were available for analysis. Conjugated bilirubin in μmol/l and the percentage value were significantly above the DHMA threshold limit......: mean 129.7 μmol/l (42-334 μmol/l) and 73% (28-97%), respectively. CONCLUSION: The total amount of conjugated bilirubin above 20 μmol/l is sufficient to require further evaluation for biliary atresia. The percentage value is unnecessary and may cause confusion. FUNDING: none. TRIAL REGISTRATION...

  13. Effects of OATP -C gene polymorphisms and low-dose rifampicin on serum bilirubin level

    Institute of Scientific and Technical Information of China (English)

    WeiZHANG; Yi-jingHE; QingLI; Hong-haoZHOU

    2005-01-01

    AIM OATP-C is a liver-specific organic anion uptake transporter and shows high affinity for bilirubin uptaking. Rifampicin has been identified as a potent inhibitor of OATP-C both in vitro and in vivo. This study was set to determine the allele frequencies of OATP-C*1a', OATP-C*1b, and OATP-C* 15 in Chinese population, and secondly, to quantitate the contribution of the OATP-C gene polymorphisms and low-dose rifampicin adminstration to the serum bilirubin level in vivo. METHODS Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) and a novel tetreprimers method were used to identify OATP-C*1a, OATP-C*1b, and OATP-C*15 genotypes.

  14. The role of bilirubin in diabetes, metabolic syndrome, and cardiovascular diseases

    Directory of Open Access Journals (Sweden)

    Libor eVitek

    2012-04-01

    Full Text Available Bilirubin belongs to a phylogenetically old superfamily of tetrapyrrolic compounds, which have multiple biological functions. Although for decades they were believed to be only a waste product of the heme catabolic pathway at best, and a potentially toxic compound at worst; recent data has convincingly demonstrated that mildly elevated serum bilirubin levels are strongly associated with a lower prevalence of oxidative stress-mediated diseases. Indeed, serum bilirubin has been consistently shown to be negatively correlated to cardiovascular diseases (CVD, as well as to CVD-related diseases and risk factors such as arterial hypertension, diabetes mellitus, metabolic syndrome, and obesity. In addition, the clinical data are strongly supported by evidence arising from both in vitro and in vivo experimental studies. This data not only shows the protective effects of bilirubin per se; but addionally, of other products of the heme catabolic pathway such as biliverdin and carbon monoxide, as well as its key enzymes (heme oxygenase and biliverdin reductase; thus, further underlining the biological impacts of this pathway. In this review, detailed information on the experimental and clinical evidence between the heme catabolic pathway and CVD, and those related diseases such as diabetes, metabolic syndrome, and obesity is provided. All of these pathological conditions represent an important threat to human civilization, being the major killers in developed countries, with a steadily increasing prevalence. Thus, it is extremely important to search for novel markers of these diseases, as well as for novel therapeutic modalities to reverse this unfavorable situation. The heme catabolic pathway seems to fulfill the criteria for both diagnostic purposes as well as for potential therapeutical interventions.

  15. Influence of bilirubin and other antioxidants on nitrergic relaxation in the pig gastric fundus.

    Science.gov (United States)

    Colpaert, E E; Lefebvre, R A

    2000-03-01

    1. The influence of several antioxidants (bilirubin, urate, ascorbate, alpha-tocopherol, glutathione (GSH), Cu/Zn superoxide dismutase (SOD) and the manganese SOD mimic EUK-8) on nitrergic relaxations induced by either exogenous nitric oxide (NO; 10(-5) M) or electrical field stimulation (4 Hz; 10 s and 3 min) was studied in the pig gastric fundus. 2. Ascorbate (5x10(-4) M), alpha-tocopherol (4x10(-4) M), SOD (300 - 1000 u ml(-1)) and EUK-8 (3x10(-4) M) did not influence the relaxations to exogenous NO. In the presence of GSH (5x10(-4) M), the short-lasting relaxation to NO became biphasic, potentiated and prolonged. Urate (4x10(-4) M) and bilirubin (2x10(-4) M) also potentiated the relaxant effect of NO. None of the antioxidants influenced the electrically evoked relaxations. 3. 6-Anilino-5,8-quinolinedione (LY83583; 10(-5) M) had no influence on nitrergic nerve stimulation but nearly abolished the relaxant response to exogenous NO. Urate and GSH completely prevented this inhibitory effect, while it was partially reversed by SOD and bilirubin. Ascorbate, alpha-tocopherol and EUK-8 were without effect. 4. Hydroquinone (10(-4) M) did not affect the electrically induced nitrergic relaxations, but markedly reduced NO-induced relaxations. The inhibition of exogenous NO by hydroquinone was completely prevented by urate and GSH. SOD and ascorbate afforded partial protection, while bilirubin, EUK-8 and alpha-tocopherol were ineffective. 5. Hydroxocobalamin (10(-4) M) inhibited relaxations to NO by 50%, but not the electrically induced responses. Full protection versus this inhibitory effect was obtained with urate, GSH and alpha-tocopherol. 6. These results strengthen the hypothesis that several endogenous antioxidant defense mechanisms, enzymatic as well as non-enzymatic, might play a role in the nitrergic neurotransmission process.

  16. Direct antioxidant properties of bilirubin andbiliverdin. Is there a role for biliverdin reductase?

    Directory of Open Access Journals (Sweden)

    Thomas eJansen

    2012-03-01

    Full Text Available Reactive oxygen species (ROS and signaling events are involved in the pathogenesis of endothelial dysfunction and represent a major contribution to vascular regulation. Molecular signaling is highly dependent on reactive oxygen species. But depending on the amount of ROS production it might have toxic or protective effects. Despite a large number of negative outcomes in large clinical trials (e.g. HOPE, HOPE-TOO, antioxidant molecules and agents are important players to influence the critical balance between production and elimination of RONS. However, chronic systemic antioxidant therapy lacks clinical efficacy, probably by interfering with important physiological redox signaling pathways. Therefore, it may be a much more promising attempt to induce intrinsic antioxidant pathways in order to increase the antioxidants not systemically but at the place of oxidative stress and complications. Among others, heme oxygenase (HO has been shown to be important for attenuating the overall production of ROS in a broad range of disease states through its ability to degrade heme and to produce carbon monoxide (CO, biliverdin/bilirubin, and the release of free iron with subsequent ferritin induction. With the present review we would like to highlight the important antioxidant role of the heme oxygenase system and especially discuss the contribution of the biliverdin, bilirubin and biliverdin reductase to these beneficial effects. The bilierdin reductase was reported to confer an antioxidant redox amplification cycle by which low, physiological bilirubin concentrations confer potent antioxidant protection via recycling of biliverdin from oxidized bilirubin by the biliverdin reductase, linking this sink for oxidants to the NADPH pool. To date the existence and role of this antioxidant redox cycle is still under debate and we present and discuss the pros and cons as well as our own findings on this topic.

  17. The antioxidant effect of free bilirubin on cumene-hydroperoxide treated human leukocytes.

    Science.gov (United States)

    Yesilkaya, A; Altinayak, R; Korgun, D K

    2000-07-01

    To examine the antioxidant effect of bilirubin (BR) on leukocyte, we treated leukocytes obtained from healthy subjects with an oxidant and various concentrations of BR. High concentrations of BR decreased thiobarbituric acid reactive substances (TBARS) and catalase activities, increased superoxide dismutase (SOD) activity, but had no effect on glutathione (GSH) concentration. Our results showed that under physiological conditions, BR has an antioxidant effect only in high concentrations.

  18. Antioxidant potential of bilirubin-accelerated wound healing in streptozotocin-induced diabetic rats.

    Science.gov (United States)

    Ram, Mahendra; Singh, Vishakha; Kumar, Dhirendra; Kumawat, Sanjay; Gopalakrishnan, Anu; Lingaraju, Madhu C; Gupta, Priyanka; Tandan, Surendra Kumar; Kumar, Dinesh

    2014-10-01

    Oxidative injury is markedly responsible for wound complications in diabetes mellitus. The biological actions of bilirubin may be relevant to prevent oxidant-mediated cell death, as bilirubin application at a low concentration scavenges reactive oxygen species. Hence, we hypothesized that topical bilirubin application might improve wound healing in diabetic rats. Diabetes was induced in adult male Wistar rats, which were divided into two groups, i.e., diabetic control and diabetic treated. Non-diabetic healthy rats were also taken as healthy control group. Wound area was measured on days 3, 7, 14, and 19 post-wounding. The levels of malondialdehyde (MDA) and reduced glutathione (GSH) and the activities of glutathione peroxidase (GPx), superoxide dismutase (SOD), and catalase (CAT) were estimated in the granulation tissue. There was a significant increase in percent wound closure in healthy control and diabetic treated rats on days 7, 14, and 19, as compared to diabetic control rats on days 7, 14, and 19. There was significant decrease in MDA levels on days 7, 14, and 19 in diabetic treated rats, as compared to diabetic control rats. Levels of GSH were significantly increased on days 3, 7, 14, and 19 in diabetic treated rats, as compared to diabetic control rats. GPx, SOD, and CAT activities were significantly higher on days 3, 7, and 14 in diabetic treated rats, as compared to diabetic control rats. The findings indicate that bilirubin is effective in reducing the oxidant status in wounds of diabetic rats which might have accelerated wound healing in these rats.

  19. Ternary complexes of albumin-Mn(II)-bilirubin and Electron Spin Resonance studies of gallstones

    CERN Document Server

    Chikvaidze, E N; Kirikashvili, I N; Mamniashvili, G I

    2009-01-01

    The stability of albumin-bilirubin complex was investigated depending on pH of solution. It was shown that the stability of complex increases in presence of Mn(II) ions. It was also investigated the paramagnetic composition of gallstones by the electron spin resonance (ESR) method. It turned out that all investigated gallstones contain a free bilirubin radical-the stable product of its radical oxidation. Accordingly the paramagnetic composition gallstones could be divided on three main types: cholesterol, brown pigment and black pigment stones. ESR spectra of cholesterol stones is singlet with g=2.003 and splitting between components 1.0 mT. At the same time the brown gallstones, besides aforementioned signal contain the ESR spectrum which is characteristics for Mn(II) ion complexes with inorganic compounds and, finally, in the black pigment stones it was found out Fe(III) and Cu(II) complexes with organic compounds and a singlet of bilirubin free radical. It is supposed that crystallization centers of gallst...

  20. Mildly elevated serum total bilirubin levels are negatively associated with carotid atherosclerosis among elderly persons with type 2 diabetes.

    Science.gov (United States)

    Kawamoto, Ryuichi; Ninomiya, Daisuke; Hasegawa, Yoichi; Kasai, Yoshihisa; Kusunoki, Tomo; Ohtsuka, Nobuyuki; Kumagi, Teru; Abe, Masanori

    2016-01-01

    Diabetes is strongly associated with several mechanisms of tissue damage such as oxidative stress. Serum bilirubin may have a beneficial role in preventing oxidative changes in cardiovascular disease (CVD). Limited information is available on whether serum bilirubin is an independent confounding factor for carotid atherosclerosis among elderly persons with type 2 diabetes. The study subjects were 169 men aged 79 ± 8 (mean ± SD) years and 205 women aged 81 ± 8 years that were enrolled consecutively from patients in the medical department. Carotid intima-media thickness (IMT) and plaque were derived via B-mode ultrasonography. Multiple linear regression analysis showed that serum total bilirubin (β = -0.160) was significantly associated with carotid IMT. Compared to subjects with a serum total bilirubin of tertile-1 (0.13-0.58 mg/dL), the multivariate-adjusted odds ratio (95% confidence interval) of carotid IMT ≥1.0 mm including plaque and carotid plaque was 0.46 (0.23-0.93) and 0.32 (0.17-0.60) in the Tertile-3 group (0.87-1.93 mg/dL), respectively. Next, data were further stratified by gender, age, smoking status, medication and prevalence of CVD. There were no significant differences in serum total bilirubin levels between selected subgroups. Our data demonstrated a negative association between serum total bilirubin and carotid atherosclerosis among elderly persons with type 2 diabetes.

  1. Usefulness of serum bilirubin levels as a biomarker for long-term clinical outcomes after percutaneous coronary intervention.

    Science.gov (United States)

    Kim, Hyun-Wook; Choi, Dong-Hyun; Lim, Leejin; Lee, Young-Min; Kang, Joon Tae; Chae, Seung Seok; Ki, Young-Jae; Song, Heesang; Koh, Young-Youp

    2015-11-01

    The aim of this study was to evaluate the prognostic value of serum total bilirubin on the development of adverse outcomes after percutaneous coronary intervention (PCI) besides high-sensitivity cardiac troponin T (hs-cTnT) and N-terminal pro-B type natriuretic peptide (NT-proBNP). Serum total bilirubin, hs-cTnT, and NT-proBNP were analyzed in 372 patients who underwent PCI. The primary endpoint was cardiac death. There were 21 events of cardiac death during a mean of 25.8 months of follow-up. When the serum total bilirubin cut-off level (median value) was set to 0.58 mg/dL using the receiver operating characteristic curve, the sensitivity was 95.2 % and the specificity was 51.0 % for differentiating between the group with cardiac death and the group without cardiac death. Kaplan-Meier analysis revealed that the lower serum total bilirubin group (bilirubin group (≥0.58 mg/dL) (10.4 vs. 0.6 %, log-rank: P = 0.0001). In conclusion, low serum total bilirubin is a predictive marker for cardiac death after PCI.

  2. Does corticosteroid treatment cause prolonged recovery and increased total bilirubin level in severe ADAMTS-13-deficient TTP patient?

    Science.gov (United States)

    Sayiner, Zeynel Abidin; Acik, Didar Yanardag; Yilmaz, Mehmet; Subari, Salih; Mete, Ayse Ozlem; Dai, M Sinan

    2015-10-01

    A 41-year-old female patient complaining of fatigue, headache, mild confusion, and rush on her lower extremities was admitted to our emergency department. Laboratory tests revealed that he had anemia, thrombocytopenia, and increased levels of indirect bilirubin and lactic dehydrogenase (LDH) in blood tests. Direct and indirect Coombs tests were negative, and fragmented erythrocytes were observed in peripheral blood smears. The patient was diagnosed with thrombotic thrombocytopenic purpura (TTP). The best supportive care was provided. Therapeutic plasma exchange (TPE) and 1 mg/kg methylprednisolone treatments were administered. On the 10th day of treatment, LDH level and fragmented red blood cells in peripheral blood smear were decreased, but his direct and indirect bilirubin levels increased despite the fact that he was treated with 1 mg/kg methylprednisolone and TPE. The patient had severe ADAMTS-13 deficiency. After discontinued steroids treatment, his bilirubin level normalized within 4 days. On the 4th day after bilirubin level normalized, vincristine treatment was administered. TPE was also continued. There was no consensus about the optimal schedule for discontinuing plasmapheresis therapy, and also we observed total bilirubin level improvement with discontinued corticosteroid treatment. In this case, corticosteroid treatment was linked with the increase of total bilirubin level in severe ADAMTS-13-deficient TTP patient.

  3. High serum total bilirubin as a protective factor against hip bone loss in healthy middle-aged men.

    Science.gov (United States)

    Kim, Beom-Jun; Koh, Jung-Min; Ahn, Seong Hee; Lee, Seung Hun; Kim, Eun Hee; Bae, Sung Jin; Kim, Hong-Kyu; Choe, Jae Won; Kim, Ghi Su

    2013-06-01

    Bilirubin is known to have a physiologic role as an antioxidant that efficiently scavenges peroxyl radicals and suppresses oxidation, and oxidative stress has detrimental effects on bone metabolism. In the present study, we performed a 3-year longitudinal study of healthy middle-aged men, investigating the association between serum total bilirubin concentrations and annualized changes in bone mineral density (BMD). The study enrolled a total of 917 Korean men aged 40 years or older who had undergone comprehensive routine health examinations with an average follow-up interval of 3 years. BMD at proximal femur sites was measured with dual-energy X-ray absorptiometry using the same equipment at baseline and follow-up. The overall mean annualized rates of bone loss at the total femur, femoral neck, and trochanter were -0.25 %/year, -0.34 %/year, and -0.44 %/year, respectively. After adjustment for potential confounders, the rates of bone loss at all proximal femur sites were significantly attenuated in a dose-response fashion across increasing bilirubin concentrations (P = 0.006-0.046). Moreover, compared to subjects in the lowest bilirubin quartile category, those in the highest bilirubin quartile category showed significantly less bone loss at all proximal femur sites after adjustment for confounding factors (P = 0.010-0.048). This study provides the first clinical evidence that serum total bilirubin could be a protective marker against future bone loss, especially in subjects without liver diseases.

  4. Bilirubin - blood

    Science.gov (United States)

    ... Gastroenterology. In: Rennie JM, ed. Rennie and Robsertson's Textbook of Neonatology. 5th ed. Philadelphia, PA: Elsevier; 2012:chap 29. Pratt DS. Liver chemistry and function tests. In: Feldman M, Friedman LS, ...

  5. Identification of heme oxygenase-1 stimulators by a convenient ELISA-based bilirubin quantification assay.

    Science.gov (United States)

    Rücker, Hannelore; Amslinger, Sabine

    2015-01-01

    The upregulation of heme oxygenase-1 (HO-1) has proven to be a useful tool for fighting inflammation. In order to identify new HO-1 inducers, an efficient screening method was developed which can provide new lead structures for drug research. We designed a simple ELISA-based HO-1 enzyme activity assay, which allows for the screening of 12 compounds in parallel in the setting of a 96-well plate. The well-established murine macrophage cell line RAW264.7 is used and only about 26µg of protein from whole cell lysates is needed for the analysis of HO-1 activity. The quantification of HO-1 activity is based on an indirect ELISA using the specific anti-bilirubin antibody 24G7 to quantify directly bilirubin in the whole cell lysate, applying a horseradish peroxidase-tagged antibody together with ortho-phenylenediamine and H2O2 for detection. The bilirubin is produced on the action of HO enzymes by converting their substrate heme to biliverdin and additional recombinant biliverdin reductase together with NADPH at pH 7.4 in buffer. This sensitive assay allows for the detection of 0.57-82pmol bilirubin per sample in whole cell lysates. Twenty-three small molecules, mainly natural products with an α,β-unsaturated carbonyl unit such as polyphenols, including flavonoids and chalcones, terpenes, an isothiocyanate, and the drug oltipraz were tested at typically 6 or 24h incubation with RAW264.7 cells. The activity of known HO-1 inducers was confirmed, while the chalcones cardamonin, flavokawain A, calythropsin, 2',3,4'-trihydroxy-4-methoxychalcone (THMC), and 2',4'-dihydroxy-3,4-dimethoxychalcone (DHDMC) were identified as new potent HO-1 inducers. The highest inductive power after 6h incubation was found at 10µM for DHDMC (6.1-fold), carnosol (3.9-fold), butein (3.1-fold), THMC (2.9-fold), and zerumbone (2.5-fold). Moreover, the time dependence of HO-1 protein production for DHDMC was compared to its enzyme activity, which was further evaluated in the presence of

  6. Mitochondrial targeting of bilirubin regulatory enzymes: An adaptive response to oxidative stress

    Energy Technology Data Exchange (ETDEWEB)

    Muhsain, Siti Nur Fadzilah, E-mail: sitinurfadzilah077@ppinang.uitm.edu.my [The University of Queensland, National Research Centre for Environmental Toxicology (Entox), 4072 Brisbane, Queensland (Australia); Faculty of Pharmacy, University Teknologi Mara (Malaysia); Lang, Matti A., E-mail: m.lang@uq.edu.au [The University of Queensland, National Research Centre for Environmental Toxicology (Entox), 4072 Brisbane, Queensland (Australia); Abu-Bakar, A' edah, E-mail: a.abubakar@uq.edu.au [The University of Queensland, National Research Centre for Environmental Toxicology (Entox), 4072 Brisbane, Queensland (Australia)

    2015-01-01

    The intracellular level of bilirubin (BR), an endogenous antioxidant that is cytotoxic at high concentrations, is tightly controlled within the optimal therapeutic range. We have recently described a concerted intracellular BR regulation by two microsomal enzymes: heme oxygenase 1 (HMOX1), essential for BR production and cytochrome P450 2A5 (CYP2A5), a BR oxidase. Herein, we describe targeting of these enzymes to hepatic mitochondria during oxidative stress. The kinetics of microsomal and mitochondrial BR oxidation were compared. Treatment of DBA/2J mice with 200 mg pyrazole/kg/day for 3 days increased hepatic intracellular protein carbonyl content and induced nucleo-translocation of Nrf2. HMOX1 and CYP2A5 proteins and activities were elevated in microsomes and mitoplasts but not the UGT1A1, a catalyst of BR glucuronidation. A CYP2A5 antibody inhibited 75% of microsomal BR oxidation. The inhibition was absent in control mitoplasts but elevated to 50% after treatment. An adrenodoxin reductase antibody did not inhibit microsomal BR oxidation but inhibited 50% of mitochondrial BR oxidation. Ascorbic acid inhibited 5% and 22% of the reaction in control and treated microsomes, respectively. In control mitoplasts the inhibition was 100%, which was reduced to 50% after treatment. Bilirubin affinity to mitochondrial and microsomal CYP2A5 enzyme is equally high. Lastly, the treatment neither released cytochrome c into cytoplasm nor dissipated membrane potential, indicating the absence of mitochondrial membrane damage. Collectively, the observations suggest that BR regulatory enzymes are recruited to mitochondria during oxidative stress and BR oxidation by mitochondrial CYP2A5 is supported by mitochondrial mono-oxygenase system. The induced recruitment potentially confers membrane protection. - Highlights: • Pyrazole induces oxidative stress in the mouse liver. • Pyrazole-induced oxidative stress induces mitochondrial targeting of key bilirubin regulatory enzymes, HMOX1

  7. The Relationship between Serum Bilirubin and Elevated Fibrotic Indices among HBV Carriers: A Cross-Sectional Study of a Chinese Population

    Directory of Open Access Journals (Sweden)

    Min Du

    2016-12-01

    Full Text Available The study probed the association between bilirubin and hepatitis B virus (HBV infection and progression. A cross-sectional analysis of 28,500 middle aged and elderly Chinese participants was performed to analyze the differences of bilirubin in terms of hepatitis B surface antigen (HBsAg positive or negative and the correlation between bilirubin and severity of hepatic fibrosis estimated by non-invasive indices. Bilirubin was significantly higher in the HBsAg (+ group than the HBsAg (− group. Higher bilirubin levels were consistently associated with elevated liver fibrosis indices among HBsAg carriers. Compared with quartile 1 of total bilirubin (TBil, the multivariable-adjusted ORs (95% CIs for elevated fibrosis indices of quartile 4 were 2.24 (95% CIs, 1.57–3.21 estimated by fibrosis 4 score (FIB-4 and 2.22 (95% CIs, 1.60–3.08 estimated by aspartate transaminase to platelet ratio index (APRI. In addition, direct bilirubin (DBil had a stronger association with elevated liver fibrosis indices than did indirect bilirubin (IBil. Furthermore, the relationship between DBil and elevated fibrosis indices was more robust among participants who were female, overweight or had central fat distribution. These findings suggested that bilirubin levels, especially DBil, were independently associated with an increased risk of increased fibrosis indices.

  8. 胆红素脑病与胆红素/血浆白蛋白比值关系探讨%The study of the relationship between bilirubin encephalopathy and bilirubin/albumin

    Institute of Scientific and Technical Information of China (English)

    刘新晖; 李贵南; 胡月圆; 张慧; 周勇

    2011-01-01

    目的 探讨胆红素脑病与血清胆红素/血浆白蛋白比值( B/A)的关系.方法 对2008年11月至2009年10月本院住院的高胆红素血症患儿进行回顾性总结,分为胆红素脑病组(病例组)与非胆红素脑病组(对照组),对住院期间的血清胆红素高峰值、血浆白蛋白以及B/A等因素之间的关系进行统计学分析.结果 共收集到2253例高胆红素血症患儿,其中88例诊断胆红素脑病.病例组B/A为(1.59±0.47),对照组B/A为(0.80±0.28),差异有统计学意义(t=474.537,P=0.000).B/A比值为0~1.0、1.1 ~2.0、2.1 ~3.0时,胆红素脑病发生率分别为0.5%、12.9%、69.2%.结论 胆红素脑病的发生与B/A比值有关,B/A越高,发生胆红素脑病的危险性越大.%Objective The relationship between the bilirubin encephalopathy and bilirubin/ albumin (B/A) is discussed. Methods Cases with hyperbilirubinemia in hospital from Dec 2008 to Oct 2009, assigned into bilirubin encephalopathy group and control group, the relationship among bilirubin peak, albumin and B/A were analyzed retrospectively. Results Of the 2253 babies with hyperbilirubinemia, A total of 88 babies developed bilirubin encephalopathy. The B/A value in bilirubin encephalopathy group was 1. 59 ±0. 47, and control group was 0. 80 ±0. 28, with significant differences between two groups (t = 474. 537, P = 0. 000). The incidence of bilirubin encephalopathy at different levels of B/A was different; 0 - 1. 0, 0. 5% ; 1. 1 -2.0, 12. 9% ; 2. 1 - 3. 0, 69. 2% . Conclusion The incidence of bilirubin encephalopathy is significantly associated with the levels of B/A.

  9. The association between indirect bilirubin levels and liver fibrosis due to chronic hepatitis C virus infection.

    Science.gov (United States)

    Cengiz, Mustafa; Yılmaz, Guldal; Ozenirler, Seren

    2014-08-01

    We proposed to evaluate the association between serum indirect bilirubin levels and liver fibrosis in patients with chronic hepatitis C (CHC) genotype 1b. Biopsy proven CHC genotype 1b patients' demographics, clinical and histopathological characteristics were evaluated. Logistic regression analysis was done to evaluate the clinical, laboratory and demographic features of the histologically proven liver fibrosis in CHC patients. A total of 112 biopsy proven CHC genotype 1b patients were enrolled into the study. Liver fibrosis scores were measured by using Ishak fibrosis scores and were divided into two groups; fibrosis scores ≤ 2 were categorized as mild fibrosis, 82 patients (73.2%), whereas fibrosis scores >2 were categorized as advanced fibrosis group, 30 patients (26.8%). Patients with advanced fibrosis had lower indirect bilirubin levels than the mild fibrosis group (0.28 ± 0.02 mg/dl vs. 0.44 ± 0.032 mg/dl, pbilirubin level was negatively correlated with advanced fibrosis scores (r=-0.416 and pbilirubin level was an independent predicting factor of advanced liver fibrosis (OR: 0.001, 95% CI: 0.0-0.005, pbilirubin levels and advanced liver fibrosis caused by CHC genotype 1b.

  10. Method for Estimating Bilirubin Isomerization Efficiency in Phototherapy to Treat Neonatal Jaundice

    Science.gov (United States)

    Lisenko, S. A.; Kugeiko, M. M.

    2014-11-01

    We propose a method for quantitative assessment of the efficacy of phototherapy to treat neonatal jaundice using the diffuse reflectance spectrum for the newborn's skin, based on the analytical dependence of the measured spectrum on the structural and morphological parameters of the skin, affecting the optical conditions in the medium, and an algorithm for rapid calculation of the bilirubin photoisomerization rate in the skin tissues as a function of the structural and morphological parameters of the skin and the wavelength of the exciting radiation. From the results of a numerical simulation of the process of radiation transport in the skin, we assess the stability of our method to variations in the scattering properties of the skin and the concentrations of its optically active chromophores (melanin, oxyhemoglobin, deoxyhemoglobin). We show that in order to achieve the maximum efficacy of phototherapy, we should use light from the range 484-496 nm. In this case, the intensity of the exciting radiation should be selected individually for each newborn according to the bilirubin photoisomerization rate characteristic for it.

  11. Effects of Calcium Ions on Thermodynamic Properties of Mixed Bilirubin/Cholesterol Monolayers

    Science.gov (United States)

    Wu, Qiong; Tang, Yu-feng; Li, Ye-min; Xie, An-jian; Shen, Yu-hua; Zhu, Jin-miao; Li, Chuan-hao

    2008-04-01

    The mixed monolayer behavior of bilirubin/cholesterol was studied through surface pressure-area (π-A) isotherms on aqueous solutions containing various concentrations of calcium ions. Based on the data of π-A isotherms, the mean area per molecule, collapse pressure, surface compressibility modulus, excess molecular areas, free energy of mixing, and excess free energy of mixing of the monolayers on different subphases were calculated. The results show an expansion in the structure of the mixed monolayer with Ca2+ in subphase, and non-ideal mixing of the components at the air/water interface is observed with positive deviation from the additivity rule in the excess molecular areas. The miscibility between the components is weakened with the increase of concentration of Ca2+ in subphase. The facts indicate the presence of coordination between Ca2+ and the two components. The mixed monolayer, in which the molar ratio of bilirubin to cholesterol is 3:2, is more stable from a thermodynamic point of view on pure water. But the stable 3:2 stoichiometry complex is destroyed with the increase of the concentration of Ca2+ in subphase. Otherwise, the mixed monolayers have more thermodynamic stability at lower surface pressure on Ca2+ subphase.

  12. Bioelectrocatalytic mediatorless dioxygen reduction at carbon ceramic electrodes modified with bilirubin oxidase

    Energy Technology Data Exchange (ETDEWEB)

    Nogala, Wojciech; Celebanska, Anna; Szot, Katarzyna [Institute of Physical Chemistry, Polish Academy of Sciences, Warsaw (Poland); Wittstock, Gunther, E-mail: gunther.wittstock@uni-oldenburg.d [Carl von Ossietzky University of Oldenburg, Faculty of Mathematics and Science, Center of Interface Science (CIS), Department of Pure and Applied Chemistry, D-26111 Oldenburg (Germany); Opallo, Marcin, E-mail: mopallo@ichf.edu.p [Institute of Physical Chemistry, Polish Academy of Sciences, Warsaw (Poland)

    2010-08-01

    Carbon ceramic electrodes were prepared by sol-gel processing of a hydrophobic precursor - methyltrimethoxysilane (MTMOS) - together with dispersed graphite microparticles according to a literature procedure. Bilirubin oxidase (BOx) was adsorbed on this electrode from buffer solution and this process was followed by atomic force microscopy (AFM). The electrodes exhibited efficient mediatorless electrocatalytic activity towards dioxygen reduction. The activity depends on the time of adsorption of the enzyme and the pH. The electrode remains active in neutral solution. The bioelectrocatalytic activity is further increased when a fraction of the carbon microparticles is replaced by sulfonated carbon nanoparticles (CNPs). This additive enhances the electrical communication between the enzyme and the electronic conductor. At pH 7 the carbon ceramic electrode modified with bilirubin oxidase retains ca. half of its highest activity. The role of the modified nanoparticles is confirmed by experiments in which a film embedded in a hydrophobic silicate matrix also exhibited efficient mediatorless biocatalytic dioxygen reduction. Scanning electrochemical microscopy (SECM) of the studied electrodes indicated a rather even distribution of the catalytic activity over the electrode surface.

  13. Spontaneous evolution in bilirubin levels predicts liver-related mortality in patients with alcoholic hepatitis.

    Directory of Open Access Journals (Sweden)

    Minjong Lee

    Full Text Available The accurate prognostic stratification of alcoholic hepatitis (AH is essential for individualized therapeutic decisions. The aim of this study was to develop a new prognostic model to predict liver-related mortality in Asian AH patients. We conducted a hospital-based, retrospective cohort study using 308 patients with AH between 1999 and 2011 (a derivation cohort and 106 patients with AH between 2005 and 2012 (a validation cohort. The Cox proportional hazards model was constructed to select significant predictors of liver-related death from the derivation cohort. A new prognostic model was internally validated using a bootstrap sampling method. The discriminative performance of this new model was compared with those of other prognostic models using a concordance index in the validation cohort. Bilirubin, prothrombin time, creatinine, potassium at admission, and a spontaneous change in bilirubin levels from day 0 to day 7 (SCBL were incorporated into a model for AH to grade the severity in an Asian patient cohort (MAGIC. For risk stratification, four risk groups were identified with cutoff scores of 29, 37, and 46 based on the different survival probabilities (P<0.001. In addition, MAGIC showed better discriminative performance for liver-related mortality than any other scoring system in the validation cohort. MAGIC can accurately predict liver-related mortality in Asian patients hospitalized for AH. Therefore, SCBL may help us decide whether patients with AH urgently require corticosteroid treatment.

  14. Photo-isomerization and oxidation of bilirubin in mammals is dependent on albumin binding.

    Science.gov (United States)

    Goncharova, Iryna; Jašprová, Jana; Vítek, Libor; Urbanová, Marie

    2015-12-01

    The bilirubin (BR) photo-conversion in the human body is a protein-dependent process; an effective photo-isomerization of the potentially neurotoxic Z,Z-BR as well as its oxidation to biliverdin in the antioxidant redox cycle is possible only when BR is bound on serum albumin. We present a novel analytical concept in the study of linear tetrapyrroles metabolic processes based on an in-depth mapping of binding sites in the structure of human serum albumin (HSA). A combination of fluorescence spectroscopy, circular dichroism (CD) spectroscopy, and molecular modeling methods was used for recognition of the binding site for BR, its derivatives (mesobilirubin and bilirubin ditaurate), and the products of the photo-isomerization and oxidation (lumirubin, biliverdin, and xanthobilirubic acid) on HSA. The CD spectra and fluorescent quenching of the Trp-HSA were used to calculate the binding constants. The results of the CD displacement experiments performed with hemin were interpreted together with the findings of molecular docking performed on the pigment-HSA complexes. We estimated that Z,Z-BR and its metabolic products bind on two independent binding sites. Our findings support the existence of a reversible antioxidant redox cycle for BR and explain an additional pathway of the photo-isomerization process (increase of HSA binding capacity; the excess free [unbound] BR can be converted and also bound to HSA).

  15. Effects of Calcium Ions on Thermodynamic Properties of Mixed Bilirubin/Cholesterol Monolayers

    Institute of Scientific and Technical Information of China (English)

    Qiong Wu; Yu-feng Tang; Ye-min Li; An-jian Xie; Yu-hua Shen; Jin-miao Zhu; Chuan-hao Li

    2008-01-01

    The mixed monolayer behavior of bilirubin/cholesterol was studied through surface pressure-area (π-A) isotherms on aqueous solutions containing various concentrations of calcium ions.Based on the data of π-A isotherms,the mean area per molecule,collapse pressure,surface compressibility modulus,excess molecular areas,free energy of mixing,and excess free energy of mixing of the monolayers on different subphases were calculated.The results show an expansion in the structure of the mixed monolayer with Ca2+ in subphase, and non-ideal mixing of the components at the air/water interface is observed with positive deviation from the additivity rule in the excess molecular areas.The miscibility between the components is weakened with the increase of concentration of Ca2+ in subphase.The facts indicate the presence of coordination between Ca2+ and the two components.The mixed monolayer,in which the molar ratio of bilirubin to cholesterol is 3:2,is more stable from a thermodynamic point of view on pure water.But the stable 3:2 stoichiometry complex is destroyed with the increase of the concentration of Ca2+ in subphase.Otherwise,the mixed monolayers have more thermodynamic stability at lower surface pressure on Ca2+ subphase.

  16. Amplifying the fluorescence of bilirubin enables the real-time detection of heme oxygenase activity.

    Science.gov (United States)

    Klemz, Roman; Mashreghi, Mir-Farzin; Spies, Claudia; Volk, Hans-Dieter; Kotsch, Katja

    2009-01-15

    Heme oxygenases (HO) are the rate-limiting enzymes in the degradation of heme to equimolar amounts of antioxidant bile pigments, the signaling molecule carbon monoxide, and ferric iron. The inducible form HO-1 confers protection on cells and tissues that mediates beneficial effects in many diseases. Consequently, measurement of the enzymatic activity is vital in the investigation of the regulatory role of HO. Here we report that the fluorescence characteristics of bilirubin in complex with serum albumin can be used for the real-time detection of HO activity in enzymatic kinetics measurements. We characterized the enzymatic activity of a truncated human HO-1 and measured the HO activity for various cell types and organs, in either the basal naive or the HO-1-induced state. The bilirubin-dependent increase in fluorescence over time monitored by this assay facilitates a very fast, sensitive, and reliable measurement of HO activity. Our approach offers the basis for a highly sensitive high-throughput screening, which provides, inter alia, the opportunity to discover new therapeutic HO-1-inducing agents.

  17. Heme oxygenase-1-derived bilirubin protects endothelial cells against high glucose-induced damage.

    Science.gov (United States)

    He, Meihua; Nitti, Mariapaola; Piras, Sabrina; Furfaro, Anna Lisa; Traverso, Nicola; Pronzato, Maria Adelaide; Mann, Giovanni E

    2015-12-01

    Hyperglycemia and diabetes are associated with endothelial cell dysfunction arising from enhanced oxidative injury, leading to the progression of diabetic vascular pathologies. The redox-sensitive transcription factor nuclear factor-E2-related factor 2 (Nrf2) is a master regulator of antioxidant genes, such as heme oxygenase-1 (HO-1), involved in cellular defenses against oxidative stress. We have investigated the pathways involved in high glucose-induced activation of HO-1 in endothelial cells and examined the molecular mechanisms underlying cytoprotection. Elevated d-glucose increased intracellular generation of reactive oxygen species (ROS), leading to nuclear translocation of Nrf2 and HO-1 expression in bovine aortic endothelial cells, with no changes in cell viability. Superoxide scavenging and inhibition of endothelial nitric oxide synthase (eNOS) abrogated upregulation of HO-1 expression by elevated glucose. Inhibition of HO-1 increased the sensitivity of endothelial cells to high glucose-mediated damage, while addition of bilirubin restored cell viability. Our findings establish that exposure of endothelial cells to high glucose leads to activation of endogenous antioxidant defense genes via the Nrf2/ARE pathway. Upregulation of HO-1 provides cytoprotection against high glucose-induced oxidative stress through the antioxidant properties of bilirubin. Modulation of the Nrf2 pathway in the early stages of diabetes may thus protect against sustained damage by hyperglycemia during progression of the disease.

  18. EFFECT OF BENZOIN RESIN ON THE SERUM BILIRUBIN LEVELS IN TEMPORARY JAUNDICE INDUCED BY PHENYLHYDRAZINE: A PRELIMINARY STUDY

    Directory of Open Access Journals (Sweden)

    RAJU.S, UMA MAHESHWARA RAO.V SREERAMULU REDDY.K, RAMYA.G, VASANTH KUMAR. G

    2013-10-01

    Full Text Available Bilirubin is the degradation product of heme, thebulk of which is derived from hemoglobin of senescenterythrocytes and hepatic hemoproteins. Bilirubin ispotentially toxic, but is normally rendered harmless bybinding to plasma albumin, and efficient hepatic clearance.Jaundice, (also known as icterus is a yellowishpigmentation of the skin, the conjunctival membranes overthe sclerae (whites of the eyes, and other mucousmembranes caused by hyperbilirubinemia (increased levelsof bilirubin in the blood. Complications of jaundiceinclude sepsis especially cholangitis, biliary cirrhosis,pancreatitis, coagulopathy, renal and liver failure.Treatment of rats with Phenylhydrazine 5 mg/ kg bodyweight for five days resulted in the development ofjaundice as BR level was found to be higher than 2 mg/dL.Bilirubin lowering potential of Benzoin ethyl alcoholextract was evaluated in temporarily jaundiced adultwistar rats. Treatment of these rats with Benzoin extractfor seven days reduced the BR level significantly to thenormal value. Whereas smaller dose (10mg/kg bodyweight resulted in the reduction in BR level from 2.51 ±0.02 to 0.90 ± 0.01 mg/dL, higher doses of 20 and 40mg/kg body weight were found to be more effective inreducing the bilirubin level from 2.54 ± 0.01 to 0.82 ±0.01 mg/dL and from 2.49±0.02 to 0.66±0.01 mg/dL,respectively. Therefore, Benzoin ethyl alcohol extract canbe used to reduce bilirubin concentration to a normal levelin jaundiced subjects.

  19. Lower carotid intima media thickness is predicted by higher serum bilirubin in both non-diabetic and Type 2 diabetic subjects

    NARCIS (Netherlands)

    Dullaart, Robin P. F.; Kappelle, Paul J.W.H.; de Vries, Rindert

    2012-01-01

    Background: Higher serum bilirubin levels may be implicated cardiovascular protection. It is unknown whether the impact of serum bilirubin on carotid artery intima media thickness (IMT), a marker of subclinical atherosclerosis, is different in diabetic subjects compared to non-diabetic subjects. We

  20. Thymoquinone, an active constituent of Nigella sativa seeds, binds with bilirubin and protects mice from hyperbilirubinemia and cyclophosphamide-induced hepatotoxicity.

    Science.gov (United States)

    Laskar, Amaj A; Khan, Masood A; Rahmani, Arshad H; Fatima, Sana; Younus, Hina

    2016-08-01

    Some reports indicate that thymoquinone (TQ), the main constituent of Nigella sativa seeds, is hepatoprotective. The aim of this study was to determine whether TQ is able to bind directly to bilirubin, and whether TQ or liposomal formulation of TQ (Lip-TQ) can reduce cyclophosphamide (CYP)-induced liver toxicity, serum bilirubin level in mice. The binding of TQ with bilirubin was studied by UV-VIS, fluorescence and Near-UV CD spectroscopy. Inhibition of binding of bilirubin to erythrocytes by TQ was also examined. To increase the in vivo efficacy, Lip-TQ was prepared and used against CYP-induced toxicity. The protective role of TQ or Lip-TQ against CYP-induced toxicity was assessed by determining the liver function parameters, the levels of superoxide dismutase (SOD) and catalase (CAT), and histological studies. It was found that TQ binds to bilirubin and significantly inhibits the binding of bilirubin to erythrocytes. Lip-TQ (10 mg/kg) significantly reduced the levels of aspartate transaminase (AST) from 254 ± 48 to 66 ± 18 IU/L (P bilirubin from 2.8 ± 0.50 to 1.24 ± 0.30 mg/dl (P bilirubin in systemic circulation in disease conditions that lead to hyperbilirubinemia and liver toxicity and hence may be used as a supplement in the treatment of liver ailments.

  1. Significance of total bilirubin/albumin in predicting bilirubin neurotoxicity%新生儿总胆红素与白蛋白比值对预测胆红素神经毒性的临床意义

    Institute of Scientific and Technical Information of China (English)

    陈清; 苏卫东; 瞿尔力; 黄育丹; 邓秀睿

    2011-01-01

    目的 探讨新生儿血清总胆红素/白蛋白在预测胆红素神经毒性方面的临床意义.方法 选择2007年5月至2010年8月住院治疗的高胆红素血症新生儿83例,检测其血清总胆红素和白蛋白等,并计算血清总胆红素/白蛋白,同时根据脑干听觉诱发电位(BAEP)的检测结果分为BAEP正常组和BAEP异常组,并对比观察.结果 BAEP异常组27例,BAEP正常组56例,BAEP 异常组的血清总胆红素、血清总胆红素/白蛋白均显著高于BAEP正常组[(356.50±59.23)μmol/L比(318.70±55.12)μmol/L、(5.02±0.49)×10-3比(4.56±0.43)×10-3],差异有统计学意义(P0.05).结论 血清总胆红素/白蛋白较血清总胆红素更能反映高胆红素血症新生儿血中游离胆红素的水平,可作为评估胆红素神经毒性危险因素的指标之一.%Objective To investigate the significance of total bilirubin/albumin in predicting bilirubin neurotoxicity.Methods Eighty-three cases with hyperbilirubinemia who treated from May 2007 to August 2010 were selected,the serum total bilirubin and albumin were detected and total bilirubin/albumin was calculated.According to brainstem auditory evoked potential(BAEP)results,the patients were divided into normal BAEP group and abnormal BAEP group and compared.Results There were 27 cases of abnormal BAEP group and 56 cases of normal BAEP group.Total bilirubin and total bilirubin/albumin in abnormal BAEP group were higher than those in normal BAEP group[(356.50±59.23)μmol/L vs.(318.70±55.12)μmol/L,(5.02±0.49)×10-3 vs.(4.56±0.43)×10-3],the differences were significant (P0.05).Conclusion Total bilirubin/albumin can reflect serum unconjugated bilirubin level of neonatus with hyperbilirubinemia better than total bilirubin,and it can can be taken as one index to evaluate the risk factors of bilirubin neurotoxicity.

  2. Subtle bilirubin-induced neurodevelopmental dysfunction (BIND) in the term and late preterm infant : Does it exist?

    NARCIS (Netherlands)

    Lunsing, Roelineke J.

    2014-01-01

    Subtle bilirubin-induced neurological dysfunction (BIND) is defined as disturbances in sensory and sensorimotor integration, central auditory processing, coordination, and muscle tone in the absence of the classical findings of kernicterus. This review is restricted to the (sensori)motor signs of BI

  3. Evaluation of Treatment Thresholds for Unconjugated Hyperbilirubinemia in Preterm Infants: Effects on Serum Bilirubin and on Hearing Loss?

    NARCIS (Netherlands)

    Hulzebos, C.V.; Dommelen, P. van; Verkerk, P.H.; Dijk, P.H.; Straaten, H.L.M. van

    2013-01-01

    Background:Severe unconjugated hyperbilirubinemia may cause deafness. In the Netherlands, 25% lower total serum bilirubin (TSB) treatment thresholds were recently implemented for preterm infants.Objective:To determine the rate of hearing loss in jaundiced preterms treated at high or at low TSB thres

  4. [Postpartal serum bilirubin levels in the newborn after induction of labour with "prostaglandin cap" or oxytocin (author's transl)].

    Science.gov (United States)

    Grünberger, W; Coradello, H; Huber, J; Husslein, P

    1981-04-01

    In the course of a prospective study the development of Serum bilirubine levels was controlled in 90 neonates. In 30 cases labour had been induced by means of intravenous oxytocin infusion, in a further 30 cases by means of local peri-cervical prostaglandine E2 (PGE2)-application. The control group consisted of 30 children, with spontaneous onset of labour. Anamnesis, duration of gravidity, course of labour and method of delivery were the same in all groups; the neonates were all treated the same. The serum bilirubine was determined fotometrically with the Greiner Selective Analyzer GSA II on the 1st, 3rd and 5th post partum day and the results assessed by the multivariant analysis according to Newman-Keuls. No differences were found between the PGE2- and the control group, the bilirubine values of the oxytocin groups were significantly higher (p less than 0.001). Icteric neonates with serum bilirubine values of greater than 12 mg% were found more than double as often in the oxytocin group than in the PGE2- group (7:3). The results indicate, that for labour induction by pharmaceuticals, local application of PGE2 by means of a portio cap should be favored over intravenous oxytocin administration.

  5. The Research of Bilirubin Levels in Neonatal Cerebrospinal Fluid in the Diagnosis of Bilirubin Encephalopathy%新生儿脑脊液胆红素在胆红素脑病诊断中的研究

    Institute of Scientific and Technical Information of China (English)

    孙路璐; 金玉莲; 刘光辉; 张健; 郑洪

    2013-01-01

    Objective To investigate the value of concentration of bilirubin in cerebrospinal fluid for early diagnosis of neonatal bilirubin encephalopathy.Methods 34 cases with bilirubin encephalopathy and 37 cases with non-bilirubin encephalopathy as control group were chosen from February 2011 to October 2012.The concentrations of unconjugated bilirubin in cerebrospinal fluid and unconjugated bilirubin in serum of two groups were compared.According to the ROC curve,their critical value,sensitivity,specificity,positive predictive value and negative predictive value in the diagnosis of bilirubin encephalopathy were analyzed.Results The unconjugated bilirubin in cerebrospinal fluid in the bilirubin encephalopathy group (13.88 ± 5.03)μmol/L was significant higher than that in the control group (5.83 ± 4.30)μmol/L(P < 0.01),there was statistical significance in difference (P < 0.01).The area under curve of unconjugated bilirubin in cerebrospinal fluid(0.909) was larger than that of unconjugated bilirubin in serum(0.692),according to the ROC curve.When the critical value was 9.55 μmol/L,the sensitivity and specificity of unconjugated bilirubin in cerebrospinal fluid in the diagnosis of neonatal bilirubin encephalopathy were 86.7% and 93.9%,respectively.Conclusion Unconjugated bilirubin in cerebros-pinal fluid value was a good indicator for predicting bilirubin encephalopathy and it was helpful to provide information for rational clinical treatment of hyperbilirubinemia.%目的 探讨高胆红素血症新生儿脑脊液未结合胆红素水平对胆红素脑病的早期诊断价值.方法 以2011年2月-2012年10月入院的34例胆红素脑病患儿(病例组)和37例单纯高胆红素血症患儿(对照组)为研究对象,比较两组脑脊液及血清未结合胆红素水平,并绘制ROC曲线,计算脑脊液未结合胆红素及血清未结合胆红素水平在诊断胆红素脑病中的临界值、灵敏度、特异度、阳性预

  6. The Evolving Landscape of Neurotoxicity by Unconjugated Bilirubin: Role of Glial Cells and Inflammation

    Directory of Open Access Journals (Sweden)

    Dora eBrites

    2012-05-01

    Full Text Available Unconjugated hyperbilirubinemia is a common condition in the first week of postnatal life. Although generally harmless, some neonates may develop very high levels of unconjugated bilirubin (UCB, which may surpass the protective mechanisms of the brain at preventing UCB accumulation. In this case, both short-term and long-term neurodevelopmental disabilities, such as acute and chronic UCB encephalopathy, known as kernicterus, or more subtle alterations designed as bilirubin-induced neurological dysfunction (BIND may be produced. There is a tremendous variability in babies’ vulnerability towards UCB for reasons not yet explained, but preterm birth, sepsis, hypoxia and haemolytic disease are comprised as risk factors. Therefore, UCB levels and neurological abnormalities are not strictly correlated. Even nowadays, the mechanisms of UCB neurotoxicity are still unclear, as are specific biomarkers, and little is known about lasting sequelae attributable to hyperbilirubinemia. On autopsy, UCB was shown to be within neurons, neuronal processes and microglia, and to produce loss of neurons, demyelination and gliosis. In isolated cell cultures, UCB was shown to impair neuronal arborization and to induce the release of proinflammatory cytokines from microglia and astrocytes. However, cell dependent-sensitivity to UCB toxicity and the role of each nerve cell type remain understood. This review provides a comprehensive insight into cell susceptibilities and molecular targets of UCB in neurons, astrocytes, and oligodendrocytes, and on phenotypic and functional responses of microglia to UCB. Interplay among glia elements and cross-talk with neurons, with a special emphasis in the UCB-induced immunostimulation, and the role of sepsis in BIND pathogenesis are highlighted. New and interesting data on the anti-inflammatory and antioxidant activities of different pharmacological agents are also presented, as novel and promising additional therapeutic approaches to

  7. Effects of Bilirubin on Alveolar Macrophages in Rats with Emphysema and Expression of iNOS and NO in Them

    Institute of Scientific and Technical Information of China (English)

    李建强; 赵卉; 宋满景; 徐永健; 张珍祥

    2004-01-01

    To explore the effects of bilirubin on alveolar macrophages (AM) and expression of iNOS and NO in them in emphysema model, the rats were pretreated with bilirubin before exposed to smoke. AM were isolated from bronchoalveolar lavage fluid (BALF) and cultured. Pathological microscopic examination of AM and immunohistochemical analysis of iNOS were performed. Nitric oxide (NO) content in the samples was determined by nitrate reductase technique. The results showed both alveoli and alveolar septum appeared normal in size and shape in normal group. AM showed kidney-shaped nucleus and were rich in Golgi complexes and primary lysosomes in the cytoplasm. The inner membrane of mitochondrion was continuous. Most cristae of the mitochondria were intact. In model group, the alveoli were expanded, ruptured and bullaes were formed. Both the population and sizes of AM increased significantly. Secondary lysosomes were rich in the cytoplasm. Deformation and pyknosis of the nucleus, swelling of the mitochondrions and rupture of the inner mitochondrial membrane could also be seen. At high magnification, most of the mitochondrial cristae were broken, or completely lost at certain points. In bilirubin group, alveoli partly expanded and the population of AM also increased, with morphological changes being slighter than that in model group. Both NO contents and expression of iNOS in model group were higher than those in normal group (P<0.05). In bilirubin group the two indice were lower than those in model group (P<0.05). Our findings suggested that high expression of iNOS and high NO content in AM accelerate the development of emphysema associated with smoking in rats. Bilirubin may exert protective effects on AM and retards the development of emphysema in rats.

  8. Transcutaneous bilirubin--comparing the accuracy of BiliChek(R) and JM 103(R) in a regional postnatal unit.

    LENUS (Irish Health Repository)

    Qualter, Yvonne M

    2012-01-31

    OBJECTIVE: Transcutaneous bilirubin (TcB) has the potential to reduce serum bilirubin sampling. During a recent survey on the use of TcB in postnatal units in the Republic of Ireland, we identified that only 58% of the 19 units were using TcB and that only two devices were in use, the BiliChek(R) and JM 103(R). We aimed to evaluate and compare these two devices in a regional postnatal unit. METHODS: To evaluate and compare the accuracy of the BiliChek(R) and JM 103(R), we studied simultaneous TcB and total serum bilirubin (TSB) measurements from a population of jaundiced term and near term infants. We evaluated each device with regard to correlation with TSB and potential to safely reduce serum bilirubin testing. RESULTS: Both TcB devices strongly correlated with TSB (r = 0.88 for BiliChek(R) and r = 0.70 for JM 103(R). The BiliChek(R) and JM 103(R) were accurate up to cut-off values of 200 mumol\\/L and 180 mumol\\/L, respectively. Using Bhutani\\'s nomogram, 100% sensitivity was achieved using the 75th percentile for BiliChek(R) and the 40th percentile for JM 103(R). CONCLUSION: Both TcB devices correlated closely with moderately increased TSB levels and are suitable screening tools to identify jaundiced infants that require a serum bilirubin, with upper limit cut-off values. Both devices reduced the need for TSB levels. We found the BiliChek(R) slightly more accurate than the JM 103(R) for our study population. TcB however, is not in widespread use.

  9. Serum Bilirubin and 6-min Walk Distance as Prognostic Predictors for Inoperable Chronic Thromboembolic Pulmonary Hypertension: A Prospective Cohort Study

    Institute of Scientific and Technical Information of China (English)

    Juan-Ni Gong; Zhen-Guo Zhai; Yuan-Hua Yang; Yan Liu; Song Gu; Tu-Guang Kuang; Wan-Mu Xie

    2015-01-01

    Background: Inoperable chronic thromboembolic pulmonary hypertension (CTEPH) is a severe clinical syndrome characterized by right cardiac failure and possibly subsequent liver dysfunction.However, whether serum markers of liver dysfunction can predict prognosis in inoperable CTEPH patients has not been determined.Our study aimed to evaluate the potential role of liver function markers (such as serum levels of transaminase, bilirubin, and gamma-glutamyl transpeptidase [GGT]) combined with 6-min walk test in the prediction of prognosis in patients with inoperable CTEPH.Methods: From June 2005 to May 2013, 77 consecutive patients with inoperable CTEPH without confounding co-morbidities were recruited for this prospective cohort study.Baseline clinical characteristics and 6-min walk distance (6MWD) results were collected.Serum biomarkers of liver function, including levels of aspartate aminotransferase, alanine aminotransferase, GGT, uric acid, and serum bilirubin, were also determined at enrollment.All-cause mortality was recorded during the follow-up period.Results: During the follow-up, 22 patients (29%) died.Cox regression analyses demonstrated that increased serum concentration of total bilirubin (hazard ratio [HR] =7.755, P < 0.001), elevated N-terminal of the prohormone brain natriuretic peptide (HR =1.001, P =0.001), decreased 6MWD (HR =0.990, P < 0.001), increased central venous pressure (HR =1.074, P =0.040), and higher pulmonary vascular resistance (HR =1.001, P =0.018) were associated with an increased risk of mortality.Serum concentrations of total bilirubin (HR =4.755, P =0.007) and 6MWD (HR =0.994, P =0.017) were independent prognostic predictors for CTEPH patients.Patients with hyperbilirubinemia (≥23.7 μ mol/L) had markedly worse survival than those with normobilirubinemia.Conclusion: Elevated serum bilirubin and decreased 6MWD are potential predictors for poor prognosis in inoperable CTEPH.

  10. NAD+ Attenuates Bilirubin-Induced Hyperexcitation in the Ventral Cochlear Nucleus by Inhibiting Excitatory Neurotransmission and Neuronal Excitability

    Science.gov (United States)

    Liang, Min; Yin, Xin-Lu; Wang, Lu-Yang; Yin, Wei-Hai; Song, Ning-Ying; Shi, Hai-Bo; Li, Chun-Yan; Yin, Shan-Kai

    2017-01-01

    Nicotinamide adenine dinucleotide (NAD+) is an important molecule with extensive biological functions in various cellular processes, including protection against cell injuries. However, little is known regarding the roles of NAD+ in neuronal excitation and excitotoxicity associated with many neurodegenerative disorders and diseases. Using patch-clamp recordings, we studied its potential effects on principal neurons in the ventral cochlear nucleus (VCN), which is particularly vulnerable to bilirubin excitotoxicity. We found that NAD+ effectively decreased the size of evoked excitatory postsynaptic currents (eEPSCs), increased paired-pulse ratio (PPR) and reversed the effect of bilirubin on eEPSCs, implicating its inhibitory effects on the presynaptic release probability (Pr). Moreover, NAD+ not only decreased the basal frequency of miniature EPSCs (mEPSCs), but also reversed bilirubin-induced increases in the frequency of mEPSCs without affecting their amplitude under either condition. Furthermore, we found that NAD+ decreased the frequency of spontaneous firing of VCN neurons as well as bilirubin-induced increases in firing frequency. Whole-cell current-clamp recordings showed that NAD+ could directly decrease the intrinsic excitability of VCN neurons in the presence of synaptic blockers, suggesting NAD+ exerts its actions in both presynaptic and postsynaptic loci. Consistent with these observations, we found that the latency of the first postsynaptic spike triggered by high-frequency train stimulation of presynaptic afferents (i.e., the auditory nerve) was prolonged by NAD+. These results collectively indicate that NAD+ suppresses presynaptic transmitter release and postsynaptic excitability, jointly weakening excitatory neurotransmission. Our findings provide a basis for the exploration of NAD+ for the prevention and treatment of bilirubin encephalopathy and excitotoxicity associated with other neurological disorders. PMID:28217084

  11. Excessive bilirubin elevation in a patient with hereditary spherocytosis and intrahepatic cholestasis.

    Science.gov (United States)

    Wree, A; Canbay, A; Müller-Beissenhirtz, H; Dechêne, A; Gerken, G; Dührsen, U; Lammert, F; Nückel, H

    2011-08-01

    Hereditary spherocytosis is a common hemolytic anemia with an estimated incidence of 1 / 2500 births. It is caused by a molecular defect in one or more of the proteins of the red blood cell cytoskeleton. Mutations in the ABCB11 gene, encoding the bile salt export pump, can entail progressive familial intrahepatic cholestasis and benign recurred intrahepatic cholestasis. A 18 year old Turkish patient with hereditary spherocytosis was admitted to hospital with pruritus and severe jaundice. Ultrasound examination presented stones in gallbladder and bile duct. After endoscopic retrograde cholangiography with extraction of small bile duct stones abdominal pain resolved and liver enzymes normalized within a few days, but bilirubin and bile acids remained highly elevated. Liver biopsy revealed a severe canalicular cholestasis. Genetic analysis showed the compound heterozygous variants ABCB11 A 444V and 3084A > G. Treatment with ursodesoxycholic acid and intermittent therapy with prednisone reduced pruritus and jaundice with concomitant improvement of blood test. Here we report the first case of a patient with combined hereditary spherocytosis and compound heterozygous ABCB11 gene variants predisposing to intrahepatic cholestasis. Therefore, patients with hemolytic disorders should be investigated for bile acid transporter diseases in case of hyperbilirubinemia and severe cholestasis.

  12. Analysis of binding ability of two tetramethylpyridylporphyrins to albumin and its complex with bilirubin

    Science.gov (United States)

    Solomonov, Alexey V.; Shipitsyna, Maria K.; Vashurin, Arthur S.; Rumyantsev, Evgeniy V.; Timin, Alexander S.; Ivanov, Sergey P.

    2016-11-01

    An interaction between 5,10,15,20-tetrakis-(N-methyl-x-pyridyl)porphyrins, x = 2; 4 (TMPyPs) with bovine serum albumin (BSA) and its bilirubin (BR) complex was investigated by UV-Viz and fluorescence spectroscopy under imitated physiological conditions involving molecular docking studies. The parameters of forming intermolecular complexes (binding constants, quenching rate constants, quenching sphere radius etc.) were determined. It was showed that the interaction between proteins and TMPyPs occurs via static quenching of protein fluorescence and has predominantly hydrophobic and electrostatic character. It was revealed that obtained complexes are relatively stable, but in the case of TMPyP4 binding with proteins occurs better than TMPyP2. Nevertheless, both TMPyPs have better binding ability with free protein compared to BRBSA at the same time. The influence of TMPyPs on the conformational changes in protein molecules was studied using synchronous fluorescence spectroscopy. It was found that there is no competition of BR with TMPyPs for binging sites on protein molecule and BR displacement does not occur. Molecular docking calculations have showed that TMPyPs can bind with albumin via tryptophan residue in the hydrophilic binding site of protein molecule but it is not one possible interaction way.

  13. Decolorization and biodegradation of remazol brilliant blue R by bilirubin oxidase.

    Science.gov (United States)

    Liu, Youxun; Huang, Juan; Zhang, Xiaoyu

    2009-12-01

    The dye-decolorizing potential of bilirubin oxidase (BOX) was demonstrated for an anthraquinone dye, remazol brilliant blue R (RBBR). The dye was decolorized 40% within 4 h by the BOX alone, whereas it was more efficient in the presence of 2, 2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), showing 91.5% decolorization within 25 min. The effects of operational parameters on decolorization were examined. The results showed that the decolorization efficiency decreased with increasing RBBR concentration, and a marked inhibition effect was exhibited when the dye concentrations were above 100 mg l(-1). The optimum temperature for enzymatic decolorization was 40 degrees C. BOX showed efficient decolorization of the dye with a wide pH range of 5-8.5. The maximum decolorization activity occurred at pH 8 with ABTS and at pH 5 without ABTS. Analysis of RBBR ultraviolet and visible (UV-VIS) spectra after BOX treatment indicated that the decolorization of RBBR was due to biodegradation. Our results suggested that ABTS can serve as an electron mediator to facilitate the oxidation of RBBR, and the BOX-ABTS mediator-involved dye decolorization mechanism was similar to that of laccase. Operation over a wide range of pH and efficient decolorization suggested that the BOX can be used to decolorize synthetic dyes from effluents, especially for anthraquinonic dyes.

  14. Bilirubin oxidation end products directly alter K+ channels important in the regulation of vascular tone.

    Science.gov (United States)

    Hou, Shangwei; Xu, Rong; Clark, Joseph F; Wurster, William L; Heinemann, Stefan H; Hoshi, Toshinori

    2011-01-01

    The exact etiology of delayed cerebral vasospasm following cerebral hemorrhage is not clear, but a family of compounds termed 'bilirubin oxidation end products (BOXes)' derived from heme has been implicated. As proper regulation of vascular smooth muscle tone involves large-conductance Ca(2+)- and voltage-dependent Slo1 K(+) (BK, maxiK, K(Ca)1.1) channels, we examined whether BOXes altered functional properties of the channel. Electrophysiological measurements of Slo1 channels heterologously expressed in a human cell line and of native mouse BK channels in isolated cerebral myocytes showed that BOXes markedly diminished open probability. Biophysically, BOXes specifically stabilized the conformations of the channel with its ion conduction gate closed. The results of chemical amino-acid modifications and molecular mutagenesis together suggest that two specific lysine residues in the structural element linking the transmembrane ion-permeation domain to the carboxyl cytosolic domain of the Slo1 channel are critical in determining the sensitivity of the channel to BOXes. Inhibition of Slo1 BK channels by BOXes may contribute to the development of delayed cerebral vasospasm following brain hemorrhage.

  15. Sixth hour transcutaneous bilirubin predicting significant hyperbilirubinemia in ABO incompatible neonates

    Institute of Scientific and Technical Information of China (English)

    Ramesh Y Bhat; Pavan CG Kumar

    2014-01-01

    Background: Neonates with ABO hemolytic disease are at greater risk for developing significant hyperbilirubinemia. We aimed to determine whether sixth hour transcutaneous bilirubin (TcB) could predict such a risk. Methods: TcB measurements were obtained at the 6th hour of life in blood group A or B neonates born to blood group O, rhesus factor compatible mothers. Subsequent hyperbilirubinemia was monitored and considered significant if a neonate required phototherapy/exchange transfusion. The predictive role of sixth hour TcB was estimated. Results: Of 144 ABO incompatible neonates, 41(OA, 24; O-B, 17) had significant hyperbilirubinemia. Mean sixth hour TcB was significantly higher among neonates who developed significant hyperbilirubinemia than those who did not (5.83±1.35 mg/dL vs. 3.65±0.96 mg/dL, P4 mg/dL had the highest sensitivity of 93.5% and >6 mg/dL had the highest specifi city of 99%. Area under receiver operating characteristic curve was 0.898. Conclusion: Sixth hour TcB predicts subsequent significant hyperbilirubinemia in ABO incompatible neonates.

  16. Bilirubin oxidase based enzymatic air-breathing cathode: Operation under pristine and contaminated conditions.

    Science.gov (United States)

    Santoro, Carlo; Babanova, Sofia; Erable, Benjamin; Schuler, Andrew; Atanassov, Plamen

    2016-04-01

    The performance of bilirubin oxidase (BOx) based air breathing cathode was constantly monitored over 45 days. The effect of electrolyte composition on the cathode oxygen reduction reaction (ORR) output was investigated. Particularly, deactivation of the electrocatalytic activity of the enzyme in phosphate buffer saline (PBS) solution and in activated sludge (AS) was evaluated. The greatest drop in current density was observed during the first 3 days of constant operation with a decrease of ~60 μA cm(-2) day(-1). The rate of decrease slowed to ~10 μA cm(-2) day(-1) (day 3 to 9) and then to ~1.5 μA cm(-2)day(-1) thereafter (day 9 to 45). Despite the constant decrease in output, the BOx cathode generated residual current after 45 days operations with an open circuit potential (OCP) of 475 mV vs. Ag/AgCl. Enzyme deactivation was also studied in AS to simulate an environment close to the real waste operation with pollutants, solid particles and bacteria. The presence of low-molecular weight soluble contaminants was identified as the main reason for an immediate enzymatic deactivation within few hours of cathode operation. The presence of solid particles and bacteria does not affect the natural degradation of the enzyme.

  17. Characterization of erythrosine B binding to bovine serum albumin and bilirubin displacement.

    Science.gov (United States)

    Mathavan, Vinodaran M K; Boh, Boon Kim; Tayyab, Saad

    2009-08-01

    The interaction of crythrosine B (ErB), a commonly used dye for coloring foods and drinks, with bovine scrum albumin (BSA) was investigated both in the absence and presence of bilirubin (BR) using absorption and absorption difference spectroscopy. ErB binding to BSA was reflected from a significant red shift of 11 nm in the absorption maximum of ErB (527 nm) with the change in absorbance at lamdamax. Analysis of absorption difference spectroscopic titration results of BSA with increasing concentrations of ErB3 using Benesi-Hildebrand equation gave the association constant, K as 6.9 x 10(4) M(-1). BR displacing action of ErB was revealed by a significant blue shift in the absorption maximum, accompanied by a decrease in absorbance difference at lamdamax in the difference spectrum of BR-BSA complex upon addition of increasing concentrations of ErB. This was further substantiated by fluorescence spectroscopy, as addition of increasing concentrations of ErB to BR-BSA complex caused a significant decrease in fluoresccnce at 510 nm. The results suggest that ErB binds to a site in the vicinity of BR binding site on BSA. Therefore, intake of ErB may increase the risk of hyperbilirubinemia in the healthy subjects.

  18. Is bilirubin able to affect the cell cycle in Gunn rat brain? - An in vivo and in vitro study -

    OpenAIRE

    Robert, Maria Celeste

    2012-01-01

    The hyperbilirubinemic jj Gunn rat is a well established animal model for Crigler-Najjar type I Syndrome and neonatal jaundice. Similarly to humans, they present neurological deficits and what is more a marked cerebellar hypoplasia with a prominent loss and degeneration of Purkinje cells and granule neurons. Since high levels of bilirubin have been proven to arrest the cell cycle progression, we addressed the question if the cerebellar hypoplasia observed in the hyperbilirubinemic Gunn rat co...

  19. Effect of bilirubin on expression and localization of PGP and Mrp1 in the central nervous system

    OpenAIRE

    Gazzin, Silvia

    2008-01-01

    INTRODUZIONE A basse concentrazioni la bilirubina non coniugata (unconjugated bilirubin, UCB) prodotta dalla degradazione dell’emoglobina, sembra essere un potente anti-ossidante, mentre è estremamente dannosa ad alte concentrazioni, causando encefalopatia nei neonati con severo ittero. Il 70% dei bambini che presentano kernittero muoiono entro sette giorni di vita, mentre il 30% dei sopravvissuti manifesta irreversibili conseguenze come sordità, ritardo mentale e danni cerebrali perman...

  20. Analysis of observed values of glucose, creatinine, bilirubin and ALT examinations in Blood(Serum,Plasma) in hospital population

    OpenAIRE

    Soni, Heratkumar D.; Kapadia, Manisha P.; Modi, Kamal R.; Patel, Shaileshkumar M.; Saxena, Puneet

    2015-01-01

    Objectives: Find reference range by Hoffman's method and compare it with published reference range in standard textbooks.Method: Observed values (results) of glucose (FBS, RBS and PP2BS), creatinine, bilirubin (total and direct) and ALT were collected from LIS of biochemistry section of New civil hospital surat laboratory services. Data were analysed using computarised hoffman's method as described by Alex K, Claudiu B and David WS2. Reference range derived by computarised hoffman's method we...

  1. The Effects of Field Massage Technique on Bilirubin Level and the Number of Defecations in Preterm Infants

    Directory of Open Access Journals (Sweden)

    Soheila Karbandi

    2015-12-01

    Full Text Available Background: Hyperbilirubinemia is a common physiological problem in approximately 80% of preterm infants during the first week after birth. Increase in bowel movements reduces enterohepatic circulation and increases bilirubin excretion. Aim: This study aimed to evaluate the effects of Field massage technique on bilirubin level and the number of defecations in preterm infants Method: This clinical trial was performed on 80 preterm infants aged 30-36 weeks, who were hospitalized in neonatal intensive care units of Qaem, Imam Reza, and Ommolbanin hospitals of Mashhad, Iran, in 2011. The enrolled infants were randomized into intervention and control groups. The control group received the routine care, and the intervention group received a 15-minute massage twice a day (morning and evening, for five consecutive days. Field massage technique was applied by the researcher. The number of defecations and cutaneous bilirubin level were recorded on a daily basis until the sixth day after birth. Independent t-test and Mann-Whitney U test were performed to analyze the data, using SPSS version 14. Results: The mean age of the intervention and control groups was 17.2±4.5 and 17.1±4.5 hours, respectively. The mean level of cutaneous bilirubin in the intervention and control groups on the first and sixth days were not significantly different (10.7±1.5, 10.8±1.4, 13.4±2.0, and 13.4±2.6, respectively; the first day: P=0.67, the sixth day: P=0.98. The number of defecations on the fourth (P=0.01, fifth (P

  2. Inverse association between serum total bilirubin levels and diabetic peripheral neuropathy in patients with type 2 diabetes.

    Science.gov (United States)

    Kim, Eun Sook; Lee, Sung Won; Mo, Eun Young; Moon, Sung Dae; Han, Je Ho

    2015-11-01

    Several studies have suggested that bilirubin, a potent innate antioxidant, plays a protective role against cardiovascular and microvascular disease. This study investigated the association between serum concentrations of total bilirubin (TB) and the presence of diabetic peripheral neuropathy (DPN) in Korean diabetic patients. This cross-sectional study involved 1207 patients aged more than 30 years with type 2 diabetes. DPN was assessed according to clinical symptoms and physical examinations using Michigan Neuropathy Screening Instrument examination score, 10-g monofilament sensation, and current perception threshold. The subjects were stratified into gender-specific tertiles based on TB values, and the relationship between the TB values and DPN was analyzed. Compared with patients within the lowest TB tertile, those with higher TB levels consisted of patients with shorter duration of diabetes, lower HbA1c, better renal function, and less autonomic neuropathy, retinopathy, and albuminuria. Serum TB levels were inversely associated with DPN. In multivariate analysis for the development of DPN after adjusting for potential confounding factors including retinopathy, albuminuria, and autonomic neuropathy, the TB levels were inversely associated with the presence of DPN, both as a continuous variable [odds ratio (OR) per log standard deviation (SD) 0.79; 95% confidence interval (CI) 0.65-0.97; P = 0.022] and when categorized in tertiles (the highest vs. the lowest tertile; OR 0.63; 95% CI 0.40-0.99; P = 0.046). Low serum bilirubin levels are significantly associated with DPN, independently of classic risk factors and other microvascular complications. Further investigation is necessary to determine whether serum bilirubin has a prognostic significance on DPN.

  3. Studies on preparing and adsorption property of grafting terpolymer microbeads of PEI-GMA/AM/MBA for bilirubin.

    Science.gov (United States)

    Gao, Baojiao; Lei, Haibo; Jiang, Liding; Zhu, Yong

    2007-06-15

    Crosslinking copolymer microbeads with a diameter range of 100-150 microm were synthesized by suspension copolymerization of glycidyl methacrylate (GMA), acrylamide (AM) and N,N'-methylene bisacrylamide (MBA). Subsequently, polyethyleneimine (PEI) was grafted on the surfaces of the terpolymer microbeads GMA/AM/MBA via the ring-opening reaction of the epoxy groups, and the grafting microbeads PEI-GMA/AM/MBA were prepared. In this paper, the adsorption property of the grafting microbeads for bilirubin was mainly investigated, and the effects of various factors, such as pH value, ionic strength and grafting degree of PEI on the surface of grafting microbeads and the adsorption capacity of the grafting microbeads for bilirubin were examined. The batch adsorption experiment results show that by right of the action of grafted polyamine macromolecules PEI, the grafting microbeads PEI-GMA/AM/MBA have quite strong adsorption ability for bilirubin; the isotherm adsorption conforms to Freundlich equation. The pH value of the medium affects the adsorption capacity greatly, As in the nearly neutral solutions with pH 6, the grafting microbeads have the strongest adsorption ability for bilirubin, whereas in acidic and basic solutions their adsorption ability is weak. The ionic strength hardly affects the adsorption ability of the grafting microbeads. The grafting degree of PEI on the surfaces of the grafting microbeads also has a great effect on the adsorption capacity, and higher the grafting degree of PEI on the surface of the microbead PEI-GMA/AM/MBA, the stronger is the adsorption ability of the microbeads.

  4. Prognostic significance of pretreatment serum levels of albumin, LDH and total bilirubin in patients with non-metastatic breast cancer.

    Science.gov (United States)

    Liu, Xiaoan; Meng, Qing H; Ye, Yuanqing; Hildebrandt, Michelle A T; Gu, Jian; Wu, Xifeng

    2015-02-01

    Liver function tests (LFTs) have been reported as independent predictors of non-liver disease-related morbidity and mortality in general population and cancer patients. In this study, we evaluated the relationship between pretreatment serum LFTs and overall survival (OS) in non-metastatic Caucasian breast cancer patients. Seven LFTs, including albumin, alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, lactate dehydrogenase (LDH), total bilirubin and total protein, were measured in pretreatment serum from 2425 female Caucasian patients with newly diagnosed, histologically confirmed non-metastatic invasive breast cancer. Multivariate Cox model was used to estimate hazard ratio (HR) and 95% confidence interval (CI) for the association of individual LFTs with 5-year OS while adjusting for age, smoking status, pathological characteristics and treatment regimen. We found that serum albumin, LDH and total bilirubin were significantly associated with 5-year OS in multivariate Cox analyses. Patients with higher albumin level exhibited 45% reduced risk of death (HR = 0.55, 95% CI: 0.40-0.75) compared with those with lower albumin level. Patients with higher total bilirubin level had a nearly 40% reduction in the risk of death (HR = 0.62, 95% CI: 0.45-0.85) and patients with higher LDH levels had a 1.42-fold increased risk of death (HR = 1.42, 95% CI: 1.08-1.88). Furthermore, cumulative analysis showed a significant dose-response trend of significantly increasing risk of death with increasing number of unfavorable LFT levels. Our result highlighted the potential of using pretreatment serum levels of albumin, LDH and total bilirubin as prognostic factors for OS in patients with non-metastatic breast cancer.

  5. Research Advances. Image Pinpoints All 5 Million Atoms in Viral Coat; Bilirubin, "Animals-Only" Pigment, Found in Plants; New Evidence Shows Humans Make Salicylic Acid

    Science.gov (United States)

    King, Angela G.

    2009-08-01

    Recent "firsts" in chemical research: image of a viral capsid pinpointing 5 million atoms; isolation and identification of an "animal" pigment, bilirubin, from a plant source; evidence that humans make salicylic acid.

  6. Investigation of the potential modulatory effect of biliverdin, carbon monoxide and bilirubin on nitrergic neurotransmission in the pig gastric fundus.

    Science.gov (United States)

    Colpaert, Erwin E; Timmermans, Jean-Pierre; Lefebvre, Romain A

    2002-12-20

    In porcine gastric fundus, we have investigated the colocalization of the bile pigment biosynthetic enzymes heme oxygenase-2 and biliverdin reductase with neuronal nitric oxide synthase (nNOS), the effect of carbon monoxide (CO) on fundic circular smooth muscle and the possible modulatory effect of the bile pigments biliverdin and bilirubin on CO-mediated relaxations and on nitrergic relaxation. Heme oxygenase-2 and biliverdin reductase immunoreactivity was present in all nNOS containing myenteric neurons. CO induced a concentration-dependent relaxation of fundic circular smooth muscle strips, which was completely blocked by the specific guanylate cyclase inhibitor 1H-(1,2,4)oxadiazolo(4,3-a)quinoxalin-1-one (ODQ). 3-(5'-hydroxymethyl-2'-furyl)-1-benzylindazole (YC-1), biliverdin and bilirubin strongly enhanced the amplitude of the CO-induced relaxation. Tin protoporphyrin had no effect on electrically induced nitrergic relaxation, but spectrophotometric analysis learned that incubation of porcine gastric fundus circular muscle strips with tin protoporphyrin did not influence heme oxygenase activity. In conclusion, our data suggest that nitrergic neurons in the pig gastric fundus are able to produce biliverdin and bilirubin, and that these agents potentiate the relaxant effect of CO, which is formed concomitantly with biliverdin by heme oxygenase-2.

  7. Human serum albumin-stabilized gold nanoclusters act as an electron transfer bridge supporting specific electrocatalysis of bilirubin useful for biosensing applications.

    Science.gov (United States)

    Santhosh, Mallesh; Chinnadayyala, Somasekhar R; Singh, Naveen K; Goswami, Pranab

    2016-10-01

    Human serum albumin (HSA)-stabilized Au18 nanoclusters (AuNCs) were synthesized and chemically immobilized on an Indium tin oxide (ITO) plate. The assembly process was characterized by advanced electrochemical and spectroscopic techniques. The bare ITO electrode generated three irreversible oxidation peaks, whereas the HSA-AuNC-modified electrode produced a pair of redox peaks for bilirubin at a formal potential of 0.27V (vs. Ag/AgCl). However, the native HSA protein immobilized on the ITO electrode failed to produce any redox peak for bilirubin. The results indicate that the AuNCs present in HSA act as electron transfer bridge between bilirubin and the ITO plate. Docking studies of AuNC with HSA revealed that the best docked structure of the nanocluster is located around the vicinity of the bilirubin binding site, with an orientation that allows specific oxidation. When the HSA-AuNC-modified electrode was employed for the detection of bilirubin using chronoamperometry at 0.3V (vs. Ag/AgCl), a steady-state current response against bilirubin in the range of 0.2μM to 7μM, with a sensitivity of 0.34μAμM(-1) and limit of detection of 86.32nM at S/N 3, was obtained. The bioelectrode was successfully applied to measure the bilirubin content in spiked serum samples. The results indicate the feasibility of using HSA-AuNC as a biorecognition element for the detection of serum bilirubin levels using an electrochemical technique.

  8. Selective and sensitive detection of free bilirubin in blood serum using human serum albumin stabilized gold nanoclusters as fluorometric and colorimetric probe.

    Science.gov (United States)

    Santhosh, Mallesh; Chinnadayyala, Somasekhar R; Kakoti, Ankana; Goswami, Pranab

    2014-09-15

    We report here a fluorescence quenching based non-enzymatic method for sensitive and reliable detection of free bilirubin in blood serum samples using human serum albumin (HSA) stabilized gold nanoclusters (HSA-AuNCs) as fluorescent probe. The fluorescence of the nanoclusters was strongly quenched by bilirubin in a concentration dependent manner by virtue of the inherent specific interaction between bilirubin and HSA. A strong binding constant of 0.55×10(6) L mole(-1) between the HSA-AuNC and bilirubin was discerned. The nano clusters each with size ~1.0 nm (in diameter) and a core of Au18 were homogeneously distributed in HSA molecules as revealed from the respective high resolution transmission electron microscopic and mass spectroscopic studies. The fluorescence quenching phenomena which obeyed a simple static quenching mechanism, was utilized for interference free detection of bilirubin with minimum detection limit (DL) of 248±12 nM (S/N=3). The fluorescence response of HSA-AuNCs against bilirubin was practically unaltered over a wide pH (6-9) and temperature (25-50 °C) range. Additionally, peroxidase-like catalytic activity of these nanoclusters was exploited for colorimetric detection of bilirubin in serum sample with a DL of 200±19 nM by following the decrease in absorbance (at λ440 nm) of the reaction and its rate constant (Kp) of 2.57±0.63 mL μg(-1) min(-1). Both these fluorometric and colorimetric methods have been successfully used for detection of free bilirubin in blood serum samples.

  9. PREDICTION OF SIGNIFICANT NEONATAL HYPERBILIRUBINAEMIA IN HEALTHY TERM NEW BORNS USING 22-26 HOURS’ SPECIFIC SERUM BILIRUBIN – A PROSPECTIVE STUDY

    OpenAIRE

    Reddy, MR; Swathi; Sangamitra; Pratap Rao; Sugunakar; Mohd. Nasir

    2016-01-01

    INTRODUCTION Hyperbilirubinemia invariably occurs in the newborns and is discerned as clinical jaundice in nearly 50% of infants. It is a cause of concern not only for the parents but also for the paediatricians. Bilirubin production is 2-3 times higher in normal term newborns compared with adults. The colour in jaundice usually results from accumulation of unconjugated, non-polar, lipid soluble, bilirubin pigment in the skin which is formed from haemoglobin by the action of h...

  10. Bilirubin-induced neural impairment: a special focus on myelination, age-related windows of susceptibility and associated co-morbidities.

    Science.gov (United States)

    Brites, Dora; Fernandes, Adelaide

    2015-02-01

    Bilirubin-induced neurologic dysfunction (BIND) and classical kernicterus are clinical manifestations of moderate to severe hyperbilirubinemia whenever bilirubin levels exceed the capacity of the brain defensive mechanisms in preventing its entrance and cytotoxicity. In such circumstances and depending on the associated co-morbidities, bilirubin accumulation may lead to short- or long-term neurodevelopmental disabilities, which may include deficits in auditory, cognitive, and motor processing. Neuronal cell death, astrocytic reactivity, and microglia activation are part of the bilirubin-induced pathogenesis. Less understood is how abnormal growth and maturation of oligodendrocytes may impact on brain development, affecting the formation of myelin tracts. Based on in-vitro and in-vivo models, as well as in clinical cases presented here, we propose the existence of impaired myelination by bilirubin with long-term sequelae, mainly in pre-term infants. Sensitive time-windows are highlighted and centered on the different developmental-dependent impairments determined by bilirubin, and the influence of sepsis and hypoxia is reviewed.

  11. Biliverdin reductase/bilirubin mediates the anti-apoptotic effect of hypoxia in pulmonary arterial smooth muscle cells through ERK1/2 pathway

    Energy Technology Data Exchange (ETDEWEB)

    Song, Shasha [Department of Biopharmaceutical Sciences, College of Pharmacy, Harbin Medical, University (Daqing), Daqing 163319 (China); Wang, Shuang [Department of Biopharmaceutical Sciences, College of Pharmacy, Harbin Medical, University (Daqing), Daqing 163319 (China); Biopharmaceutical Key Laboratory of Heilongjiang Province, Harbin 150081 (China); Ma, Jun; Yao, Lan; Xing, Hao; Zhang, Lei; Liao, Lin [Department of Biopharmaceutical Sciences, College of Pharmacy, Harbin Medical, University (Daqing), Daqing 163319 (China); Zhu, Daling, E-mail: dalingz@yahoo.com [Department of Biopharmaceutical Sciences, College of Pharmacy, Harbin Medical, University (Daqing), Daqing 163319 (China); Biopharmaceutical Key Laboratory of Heilongjiang Province, Harbin 150081 (China)

    2013-08-01

    Inhibition of pulmonary arterial smooth muscle cell (PASMC) apoptosis induced by hypoxia plays an important role in pulmonary arterial remodeling leading to aggravate hypoxic pulmonary arterial hypertension. However, the mechanisms of hypoxia acting on PASMC apoptosis remain exclusive. Biliverdin reductase (BVR) has many essential biologic roles in physiological and pathological processes. Nevertheless, it is unclear whether the hypoxia-induced inhibition on PASMC apoptosis is mediated by BVR. In the present work, we found BVR majorly localized in PASMCs and was up-regulated in levels of protein and mRNA by hypoxia. Then we studied the contribution of BVR to anti-apoptotic response of hypoxia in PASMCs. Our results showed that siBVR, blocking generation of bilirubin, reversed the effect of hypoxia on enhancing cell survival and apoptotic protein (Bcl-2, procasepase-9, procasepase-3) expression, preventing nuclear shrinkage, DNA fragmentation and mitochondrial depolarization in starved PASMCs, which were recovered by exogenous bilirubin. Moreover, the inhibitory effect of bilirubin on PASMC apoptosis under hypoxic condition was blocked by the inhibitor of ERK1/2 pathway. Taken together, our data indicate that BVR contributes to the inhibitory process of hypoxia on PASMC apoptosis, which is mediated by bilirubin through ERK1/2 pathway. Highlights: • BVR expresses in PASMC and is up-regulated by hypoxia in protein and mRNA levels. • BVR/bilirubin contribute to the inhibitive process of hypoxia on PASMC apoptosis. • Bilirubin protects PASMC from apoptosis under hypoxia via ERK1/2 pathway.

  12. Heme Degradation by Heme Oxygenase Protects Mitochondria but Induces ER Stress via Formed Bilirubin

    Directory of Open Access Journals (Sweden)

    Andrea Müllebner

    2015-04-01

    Full Text Available Heme oxygenase (HO, in conjunction with biliverdin reductase, degrades heme to carbon monoxide, ferrous iron and bilirubin (BR; the latter is a potent antioxidant. The induced isoform HO-1 has evoked intense research interest, especially because it manifests anti-inflammatory and anti-apoptotic effects relieving acute cell stress. The mechanisms by which HO mediates the described effects are not completely clear. However, the degradation of heme, a strong pro-oxidant, and the generation of BR are considered to play key roles. The aim of this study was to determine the effects of BR on vital functions of hepatocytes focusing on mitochondria and the endoplasmic reticulum (ER. The affinity of BR to proteins is a known challenge for its exact quantification. We consider two major consequences of this affinity, namely possible analytical errors in the determination of HO activity, and biological effects of BR due to direct interaction with protein function. In order to overcome analytical bias we applied a polynomial correction accounting for the loss of BR due to its adsorption to proteins. To identify potential intracellular targets of BR we used an in vitro approach involving hepatocytes and isolated mitochondria. After verification that the hepatocytes possess HO activity at a similar level as liver tissue by using our improved post-extraction spectroscopic assay, we elucidated the effects of increased HO activity and the formed BR on mitochondrial function and the ER stress response. Our data show that BR may compromise cellular metabolism and proliferation via induction of ER stress. ER and mitochondria respond differently to elevated levels of BR and HO-activity. Mitochondria are susceptible to hemin, but active HO protects them against hemin-induced toxicity. BR at slightly elevated levels induces a stress response at the ER, resulting in a decreased proliferative and metabolic activity of hepatocytes. However, the proteins that are targeted

  13. Spectrofluorimetric quantification of bilirubin using yttrium-norfloxacin complex as a fluorescence probe in serum samples

    Energy Technology Data Exchange (ETDEWEB)

    Kamruzzaman, Mohammad; Alam, Al-Mahmnur [Department of Chemistry, Kyungpook National University, Daegu 702-701 (Korea, Republic of); Hak Lee, Sang, E-mail: shlee@knu.ac.kr [Department of Chemistry, Kyungpook National University, Daegu 702-701 (Korea, Republic of); Ho Kim, Young, E-mail: youngkim@knu.ac.kr [Research Institute of Advanced Energy Technology, Kyungpook National University, Daegu 702-701 (Korea, Republic of); Kim, Gyu-Man [School of Mechanical Engineering, Kyungpook National University, Daegu 702-701 (Korea, Republic of); Hyub Oh, Sang [Center for Gas Analysis, Korea Research Institute of Standards and Science, Daejeon 305-600 (Korea, Republic of)

    2012-11-15

    A simple and sensitive spectrofluorimetric method was developed to determine trace amounts of bilirubin (BR) using yttrium (Y{sup 3+})-norfloxacin (NFLX) complex as a fluorescence (FL) probe. NFLX can form a stable binary complex with Y{sup 3+} and markedly enhances the weak FL signal of the NFLX. The FL intensity of the Y{sup 3+}-NFLX complex decreased significantly in the presence of BR in a buffer solution at pH=7.2. Under optimal conditions, the FL intensity decreased according to the BR concentration and showed a good linear relationship in the range of 0.03-2.3 {mu}g mL{sup -1} of BR with a correlation coefficient of 0.9988. The limit of detection for the determination of BR was 2.8 ng mL{sup -1} with a relative standard deviation (RSD) of 1.55% for five replicate determination of 0.05 {mu}g mL{sup -1} BR. The presented method offers higher sensitivity with simple instrumentation and was applied successfully in detecting BR at low concentrations. Highlights: Black-Right-Pointing-Pointer Weak FL signal of NFLX was enhanced at 419 nm by forming binary complex with Y{sup 3+}. Black-Right-Pointing-Pointer The FL intensity of Y{sup 3+}-NFLX complex was quenched markedly in the presence of ATP. Black-Right-Pointing-Pointer NFLX can transfer energy to Y{sup 3+} and BR and form the Y{sup 3+}-NFLX-ATP ternary complex. Black-Right-Pointing-Pointer The reduced FL intensity of the system was correlated with the concentration of BR. Black-Right-Pointing-Pointer The method is applied to determine BR at low concentration (2.8 ng mL{sup -1}) in serum.

  14. Study on correlation among three kinds of bilirubin detection method%三种胆红素检测方法相关性研究

    Institute of Scientific and Technical Information of China (English)

    李守卫; 姚强

    2013-01-01

    目的 探讨三种胆红素检测方法的相关性.方法 选取生后7d内目测存在黄疸的住院新生儿50例,抽取血清标本用生化法测定血清总胆红素值,并同步以微量法和经皮法测定胆红素值,分别计算经皮胆红素值(TcB)与静脉血总胆红素值、末梢微量血胆红素值、静脉血总胆红素值之间的相关系数、检验其统计学意义.结果 末梢微量血胆红素检测法、经皮胆红素检测法分别与静脉血生化法均有很好的相关性(r=0.948、0.935,均P<0.01),并且末梢微量血胆红素检测法与静脉血生化法相关性更好.结论 采用微量血测定法检测胆红素值,不失为一种操作简便、结果可靠的方法.%Objective To explore which operation and the accuracy of the detection method is better by exploring the correlation among three bilirubin detection methods.Methods 50 hospitalized neonates within 7 days after birth with visual jaundice were randomly selected.The total serum bilirubin level was measured by the routine laboratory method.At the same time,the serum bilirubin level was measured by bilirubin analyzer for capillary blood and transcutaneous bilirubin(TcB) measurement,then calculated the linear correlation coefficient of TcB level and TSB level,capillary blood bilirubin level and TSB level respectively to analyze their statistical significance,and get the linear regression model at last.Results Close correlation is existed between the capillary blood bilirubin measurement and the routine laboratory method,also the transcutaneous bilirubin measurement and the routine laboratory method.Moreover,the former is better than the later.Conclusion Capillary blood bilirubin is reliable in measuring serum bilirubin level of newborns.

  15. Comparison of different methods in detection of neonatal bilirubin%不同方法对新生儿胆红素测定的比较

    Institute of Scientific and Technical Information of China (English)

    符宝铭; 韦蓉; 石明芳; 杨广林; 闫芳; 黄战; 周春浪; 欧珊

    2011-01-01

    Objective: To explore the differences of neonatal bilirubin detection with different methods, find a good method to monitor the dynamic changes of neonatal bilirubin. Methods; Three methods including automatic biochemical analyzer (biochemical method) , trace bilirubin analyzer (micromethod) and percutaneous bilirubin meter (percutaneous method) were used to detect the bilirubin levels of 90 neonates, then the differences were compared; micromethod and percutaneous method both were used to monitor the bilirubin levels of 392 normal full - term neonates at 1 ~7 days after birth dynamically, then the difference was compared. Results; There was no significant difference in measured value of bilirubin between micromethod and biochemical method ( P > 0. 05 ) ; when the level of bilirubin was less than 200 junol/L and within 201 -300 uJnol/L, there was no significant difference in measured value of bilirubin between percutaneous method and biochemical method (P >0.05); when the level of bilirubin was more than 300 jtmol/L, there was significant difference in measured value of bilirubin between percutaneous method and biochemical method (P < 0. 05) ; there was significant difference in measured value of bilirubin in neonates at 1 -7 days after birth between micromethod and percutaneous method (P <0. 05) . Conclusion; Micromethod is accurate and easy to operate in detection of neonatal bilirubin, which is superior to biochemical method and percutaneous method in dynamic monitoring of neonatal bilirubin.%目的:探讨不同方法对新生儿胆红素测定的差异,以寻找监测新生儿胆红素动态变化的好方法.方法:应用3种测定方法:全自动生化分析仪(生化法)、微量胆红素测定仪(微量法)、经皮测胆仪(经皮法)对90例新生儿进行胆红素测定并比较其差异,用微量法和经皮法同时对392例正常足月新生儿生后1~7天胆红素进行动态监测并比较其差异.结果:微量法胆红素测定值与生

  16. Inhibition of cholesterol crystallization under bilirubin deconjugation: partial characterization of mechanisms whereby infected bile accelerates pigment stone formation.

    Science.gov (United States)

    Nakai, Kuniharu; Tazuma, Susumu; Nishioka, Tomoji; Chayama, Kazuaki

    2003-06-10

    Pigment gallstones have been reported to be closely associated with biliary tract infection. We previously reported that addition of unconjugated bilirubin (UCB), which is deconjugated by beta-glucuronidase in infected bile, could enhance cholesterol crystal formation in supersaturated model bile (MB). The present study evaluated the effect of beta-glucuronidase on the processes of pigment gallstone formation and cholesterol crystallization. Supersaturated MB (taurocholate/lecithin/cholesterol at 71:18:11, a total lipid concentration of 10.0 g/dl and a cholesterol saturation index (CSI) of 2.0) and native rat bile were mixed at a ratio of 3:1. Then, mixed bile was incubated with or without beta-glucuronidase and changes of the following parameters were investigated over time: (1) the UCB/total bilirubin ratio; (2) cholesterol crystal formation; (3) the precipitate weight and the cholesterol concentration in the precipitate and supernatant; and (4) the lipid distribution of vesicles in the supernatant. Compared with beta-glucuronidase-free bile, (1) beta-glucuronidase-containing bile showed a significant increase of the UCB/total bilirubin ratio, (2) as well as a significantly longer nucleation time (96+/-17.0 vs. 114+/-20.0) and fewer cholesterol crystals. (3) The precipitate weight and the cholesterol concentration in the precipitate were significantly increased, while the cholesterol concentration in supernatant was decreased. (4) When mixed bile was incubated with beta-glucuronidase, the cholesterol concentration in the vesicles was lower than in bile without beta-glucuronidase. The precipitate weight and the cholesterol concentration in the precipitate was increased by incubation with beta-glucuronidase, while cholesterol concentration was decreased in the supernatant (especially in the vesicles). This means that bile vesicles were more stable and it was more difficult for cholesterol crystals to form. Thus, the presence of beta-glucuronidase may inhibit the

  17. 关于胆红素升高的实验室检测与临床%Detection of bilirubin: from laboratory to clinical practice

    Institute of Scientific and Technical Information of China (English)

    王豪

    2012-01-01

    Jaundice (abnormal elevation of bilirubin) is common in clinical practice.At present bilirubin is detected by measuring total bilirubin (TB) and direct bilirubin (DB) in hospital.Indirect bilirubin (IB) is the difference of TB and DB.Direct bilirubin reflects mainly the conjugated bilirubin but they are not all equivalent.Indirect bilirubin and unconjugated bilirubin are the same condition.In clinical practice,the proportion of DB (or IB) in TB is more significant than their level of elevation.The cause of jaundice could be roughly determined by analyzing the proportion of DB (or IB) in TB.The methodology and quality of bilirubin detection are quite different in hospitals in our country.The proportion of DB aud IB in TB in detection of bilirubin is also quite different in hospitals.It leads to a big puzzle to the diagnosis and differential diagnosis of jaundice for clinical doctors.It is suggested that the quality control of bilirubin detection should be strengthened in laboratory in hospital.On the bases of strict quality control of bilirubin detection,proper adjustment of the proportion of DB and IB as to make it consistiug with clinical practice and pathogenesis of diseases,and making the proportion stable for long time are also suggested.%黄疸即胆红素异常升高在临床上很常见.目前医院检测胆红素通常测定血中的总胆红素和直接胆红素,而以总胆红素减去直接胆红素得到间接胆红素的数值.直接胆红素主要反映结合胆红素,但二者并不完全等同.与此类似,间接胆红素主要反映非结合胆红素.在临床上,对黄疸的诊断和鉴别诊断,直接胆红素(或间接胆红素)占总胆红素的比例较之胆红素升高的幅度更有意义.通过分析直接或间接胆红素的比例,往往可以对黄疸的原因做出大致的诊断和鉴别诊断.但目前各家医院检测胆红素的方法和水平差别较大,不同疾病状态下所测出的直接或间接胆红素的比

  18. Cytochrome P450 2A5 and bilirubin: Mechanisms of gene regulation and cytoprotection

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Sangsoo Daniel; Antenos, Monica [Department of Biomedical Sciences, University of Guelph, Guelph, Ontario, N1G 2W1 (Canada); Squires, E. James [Department of Animal and Poultry Sciences, University of Guelph, Guelph, Ontario, N1G 2W1 (Canada); Kirby, Gordon M., E-mail: gkirby@uoguelph.ca [Department of Biomedical Sciences, University of Guelph, Guelph, Ontario, N1G 2W1 (Canada)

    2013-07-15

    Bilirubin (BR) has recently been identified as the first endogenous substrate for cytochrome P450 2A5 (CYP2A5) and it has been suggested that CYP2A5 plays a major role in BR clearance as an alternative mechanism to BR conjugation by uridine-diphosphate glucuronyltransferase 1A1. This study investigated the mechanisms of Cyp2a5 gene regulation by BR and the cytoprotective role of CYP2A5 in BR hepatotoxicity. BR induced CYP2A5 expression at the mRNA and protein levels in a dose-dependent manner in primary mouse hepatocytes. BR treatment also caused nuclear translocation of Nuclear factor-E2 p45-related factor 2 (Nrf2) in hepatocytes. In reporter assays, BR treatment of primary hepatocytes transfected with a Cyp2a5 promoter-luciferase reporter construct resulted in a 2-fold induction of Cyp2a5 reporter activity. Furthermore, cotransfection of the hepatocytes with a Nrf2 expression vector without BR treatment resulted in an increase in Cyp2a5 reporter activity of approximately 2-fold and BR treatment of Nrf2 cotransfectants further increased reporter activity by 4-fold. In addition, site-directed mutation of the ARE in the reporter construct completely abolished both the BR- and Nrf2-mediated increases in reporter activity. The cytoprotective role of CYP2A5 against BR-mediated apoptosis was also examined in Hepa 1–6 cells that lack endogenous CYP2A5. Transient overexpression of CYP2A5 partially blocked BR-induced caspase-3 cleavage in Hepa 1–6 cells. Furthermore, in vitro degradation of BR was increased by microsomes from Hepa 1–6 cells overexpressing CYP2A5 compared to control cells transfected with an empty vector. Collectively, these results suggest that Nrf2-mediated CYP2A5 transactivation in response to BR may provide an additional mechanism for adaptive cytoprotection against BR hepatotoxicity. - Highlights: • The mechanism of Cyp2a5 gene regulation by BR was investigated. • The cytoprotective role of CYP2A5 in BR hepatotoxicity was determined. • BR

  19. PREDICTION OF SIGNIFICANT NEONATAL HYPERBILIRUBINAEMIA IN HEALTHY TERM NEW BORNS USING 22-26 HOURS’ SPECIFIC SERUM BILIRUBIN – A PROSPECTIVE STUDY

    Directory of Open Access Journals (Sweden)

    Reddy

    2016-03-01

    Full Text Available INTRODUCTION Hyperbilirubinemia invariably occurs in the newborns and is discerned as clinical jaundice in nearly 50% of infants. It is a cause of concern not only for the parents but also for the paediatricians. Bilirubin production is 2-3 times higher in normal term newborns compared with adults. The colour in jaundice usually results from accumulation of unconjugated, non-polar, lipid soluble, bilirubin pigment in the skin which is formed from haemoglobin by the action of heme oxygenase, biliverdin reductase and non-enzymatic reducing agents in the reticulo-endothelial cells. AIMS & OBJECTIVE To determine hour specific serum bilirubin (22-26 hrs which will predict, subsequent significant hyperbilirubinemia in healthy term newborns. MATERIALS & METHODS A total of 250 healthy full term newborns were enrolled into the study. First bilirubin estimation (TSB 1 was estimated at 22- 26 hrs. The neonates were followed up clinically every 12 hrs for 72 hrs (till discharge. Second bilirubin estimation (TSB S was done whenever clinical suspicion of jaundice was present (usually at 72 hours. Depending upon the TSB 1 value, the infants were evaluated by using two available protocols (Arbitrary cut off value of 5 mg/dl and average value of 4.06 mg%. Sensitivity, specificity, negative and positive predictive values and likelihood ratio of the test were calculated. P-value was used to determine the level of significance. RESULTS Of 250 neonates included in the study, 13 neonates developed hyperbilirubinemia and were subjected to phototherapy. No infants with average bilirubin value of ≤4.06 mg% developed subsequent hyperbilirubinemia. However, 2 infants with arbitrary cut off value of ≤5 mg/dl developed hyperbilirubinemia. There was significant difference in TSB I value of neonates who subsequently did and those who did not developed significant hyperbilirubinemia (P-value-<0.01. The negative predictive value to these two applied protocol is very high

  20. Evaluating the role of indirect bilirubin, urobilinogen and Shine AND Lal index as an alternative screening tool for beta thalassemia minor

    Directory of Open Access Journals (Sweden)

    Ridham A. Khanderia

    2015-06-01

    Methods: The present study was conducted on 100 (n=100 subjects in blood bank, department of pathology, government medical college Rajkot, Gujarat, India. In first group 50 subjects (Thalassemia minor were selected while in second group 50 (n2=50 normal individuals from hospital staff were selected. Complete-haemogram, serum-direct, indirect and total bilirubin, urine urobilinogen and their sensitivity and specificity were calculated. Results: Of the 50 cases in test group, 41 had higher Indirect Bilirubin level (>0.7 mg/dl, 35 had high urobilinogen level (>1 mg/dl. In control group out of 50 cases, 3 had high indirect bilirubin levels, 4 had high urobilinogen levels. Indirect-bilirubin had sensitivity of 82%, specificity of 94%. Urobilinogen showed sensitivity of 70% and specificity of 92%. Conclusion: Indirect bilirubin and urine-urobilinogen is a valuable, cost-effective screening test for beta-thalassemia-trait with sensitivity and specificity comparable to RBC indices. [Int J Res Med Sci 2015; 3(3.000: 730-737

  1. Bilirubin isomer distribution in jaundiced neonates during phototherapy with LED light centered at 497 nm (turquoise) vs. 459 nm (blue)

    DEFF Research Database (Denmark)

    Ebbesen, Finn; Madsen, Poul H; Vandborg, Pernille K;

    2016-01-01

    of jaundiced neonates after 24 h of therapy with narrow-band (LED) light centered at 497 nm (turquoise) vs. 459 nm (blue), of essentially equal irradiance. MATERIALS: Eighty-three neonates (≥33 wk gestational age) with uncomplicated hyperbilirubinemia were included in the study. Forty neonates were exposed...... to light centered at 497 nm and 43 infants with light centered at 459 nm. Irradiances were 5.2 × 10(15) and 5.1 × 10(15) photons/cm(2)/s, respectively. RESULTS: After 24 h of treatment no significant differences in serum concentrations of total bilirubin isomers and Z,Z-bilirubin were observed between...... the 2 groups. Interestingly, concentrations of Z,E-bilirubin, and thus also total bilirubin isomers formed during therapy, were highest for infants receiving light centered at 459 nm, while the concentration of E,Z-bilirubin was highest for those receiving light centered at 497 nm. No significant...

  2. Bilirubin Oxidase from Myrothecium verrucaria Physically Absorbed on Graphite Electrodes. Insights into the Alternative Resting Form and the Sources of Activity Loss

    Science.gov (United States)

    Tasca, Federico; Farias, Diego; Castro, Carmen; Acuna-Rougier, Cristina; Antiochia, Riccarda

    2015-01-01

    The oxygen reduction reaction is one of the most important chemical processes in energy converting systems and living organisms. Mediator-less, direct electro-catalytic reduction of oxygen to water was achieved on spectrographite electrodes modified by physical adsorption of bilirubin oxidases from Myrothecium verrucaria. The existence of an alternative resting form of the enzyme is validated. The effect on the catalytic cycle of temperature, pH and the presence of halogens in the buffer was investigated. Previous results on the electrochemistry of bilirubin oxidase and on the impact of the presence of halogens are reviewed and reinterpreted. PMID:26196288

  3. 非结合胆红素/白蛋白比值与脑干诱发电位相关性及对胆红素脑损伤预测价值%Correlation and predictive value between non conjugated bilirubin/albumin ratio and brain stem evoked potential in predicting bilirubin brain damage

    Institute of Scientific and Technical Information of China (English)

    扈志银; 郑小亮; 户振军

    2015-01-01

    目的:研究新生儿非结合胆红素/白蛋白比值与ABR相关性及对胆红素脑损伤的预测价值,为防治提供临床依据. 方法:以2013年1月至2014年12月在庆阳市妇幼保健院新生儿病房收住的113例足月高胆红素血症患儿为观察对象,检查血清总胆红素、非结合胆红素水平、计算总胆红素/白蛋白(B/A)、非结合胆红素/白蛋白(UCB/A)比值, ABR,并监测其伴随疾病. 结果:观察时间内,113例高胆红素血症的足月新生儿中合并胆红素脑病31例,无胆红素脑病患儿82例. 分析了B/A、UCB/A及ABR诊断胆红素脑病的灵敏度、特异性及Youden指数. 提示UCB/A及ABR作为诊断胆红素脑病的指标有一定价值. 结论:UCB/A值与ABR对胆红素脑损伤有诊断价值,可作为目前临床检测新生儿胆红素脑损伤的方法.%Objective:To study the predictive value of neonatal bilirubin/albumin ratio with ABR correlation to bilirubin brain damage in order to provide the clinical basis for the prevention and control. Methods:113 cases of full-term neonatal hyperbilirubinemia in Qingyang maternal and child care from Jan 2013 to Dec 2014 were checked levels of the serum total bilirubin and the combination of bilirubin, calculates ratio of total bilirubin/albumin(B/A) and the combination of bilirubin/albumin(UCB/A), ABR and its associated with disease. Results: In the observation period, there were 31 cases of bilirubin encephalopathy and no bilirubin encephalopathy of 82 cases. The sensitivity and specificity of B/A, UCB/A, ABR and Youden index in the diagnosis of neonatal hyperbilirubinemia were analyzed. Conclusion:UCB/A value and ABR have diagnostic value to the bilirubin brain damage, which can be used as a method to detect neonatal bilirubin brain injury at present.

  4. Adjusting CA19-9 values to predict malignancy in obstructive jaundice: Influence of bilirubin and C-reactive protein

    Institute of Scientific and Technical Information of China (English)

    Gaetano La Greca; Maria Sofia; Rosario Lombardo; Saverio Latteri; Agostino Ricotta; Stefano Puleo; Domenico Russello

    2012-01-01

    AIM:To find a possible relationship between inflammation and CA19-9 tumor marker by analyzing data from patients with benign jaundice (BJ) and malignant jaundice (MJ).METHODS:All patients admitted for obstructive jaundice,in the period 2005-2009,were prospectively enrolled in the study,obtaining a total of 102 patients.On admission,all patients underwent complete standard blood test examinations including C-reactive protein (CRP),bilirubin,CA19-9.Patients were considered eligible for the study when they presented obstructive jaundice confirmed by instrumental examinations and increased serum bilirubin levels (total bilirubin > 2.0 mg/dL).The standard cut-off level for CA19-9 was 32 U/mL,whereas for CRP this was 1.5 mg/L.The CA19-9 level was adjusted by dividing it by the value of serum bilirubin or by the CRP value.The patients were divided into 2 groups,MJ and BJ,and after the adjustment a comparison between the 2 groups of patients was performed.Sensitivity,specificity and positive predictive values were calculated before and after the adjustment.RESULTS:Of the 102 patients,51 were affected by BJ and 51 by MJ.Pathologic CA19-9 levels were found in 71.7% of the patients.In the group of 51 BJ patients there were 29 (56.9%) males and 22 (43.1%) females with a median age of 66 years (range 24-96 years),whereas in the MJ group there were 24 (47%) males and 27 (53%) females,with a mean age of 70 years (range 30-92 years).Pathologic CA19-9 serum level was found in 82.3% of MJ.CRP levels were pathologic in 66.6% of the patients with BJ and in 49% with MJ.Bilirubin and CA19-9 average levels were significantly higher in MJ compared with BJ (P =0.000 and P =0.02),while the CRP level was significantly higher in BJ (P =0.000).Considering a CA19-9 cut-off level of 32 U/mL,82.3% in the MJ group and 54.9% in the BJ group were positive for CA19-9 (P =0.002).A CA19-9 cut-off of 100 U/mL increases the difference between the two groups:35.3% in BJ and 68.6

  5. Energy change in the formation of conjugated bilirubin: A possible responsive mechanism for liver cell pathology Cambio energético durante la conjugación de la bilirrubina: un posible mecanismo de lesión hepatocelular

    Directory of Open Access Journals (Sweden)

    V. Wiwanitkit

    2007-02-01

    Full Text Available Bilirubin is formed when red blood cells die and their hemoglobin is broken down within the macrophages into heme and globins. In the liver, bilirubin is conjugated with UDP-glucuronate, making it water-soluble diglucuronide. Concerning this conjugation, a molecule of bilirubin reacts with two molecules of glucoronic acid. However, the nature of this energy-consuming reaction in the formation of conjugated bilirubin has never been reported, and this can be important for its potential implication in hyperbilirubinemia. In this work, the author calculated the energy required by conjugated-bilirubin formation per molecule. The energy required for complex formation is -22 kCal/mol. The nature of this energy-producing reaction can be a good explanation. Increased energy delivery in conjugated hyperbilirubinemia in liver disease might be a responsive mechanism to hepatic damage.

  6. Increasing the catalytic activity of Bilirubin oxidase from Bacillus pumilus: Importance of host strain and chaperones proteins.

    Science.gov (United States)

    Gounel, Sébastien; Rouhana, Jad; Stines-Chaumeil, Claire; Cadet, Marine; Mano, Nicolas

    2016-07-20

    Aggregation of recombinant proteins into inclusion bodies (IBs) is the main problem of the expression of multicopper oxidase in Escherichia coli. It is usually attributed to inefficient folding of proteins due to the lack of copper and/or unavailability of chaperone proteins. The general strategies reported to overcome this issue have been focused on increasing the intracellular copper concentration. Here we report a complementary method to optimize the expression in E. coli of a promising Bilirubin oxidase (BOD) isolated from Bacillus pumilus. First, as this BOD has a disulfide bridge, we switched E.coli strain from BL21 (DE3) to Origami B (DE3), known to promote the formation of disulfide bridges in the bacterial cytoplasm. In a second step, we investigate the effect of co-expression of chaperone proteins on the protein production and specific activity. Our strategy allowed increasing the final amount of enzyme by 858% and its catalytic rate constant by 83%.

  7. Indicators of inflammation and cellular damage in chronic asymptomatic or oligosymptomatic alcoholics: correlation with alteration of bilirubin and hepatic and pancreatic enzymes

    Directory of Open Access Journals (Sweden)

    Borini Paulo

    1999-01-01

    Full Text Available Biochemical and hematimetric indicators of inflammation and cell damage were correlated with bilirubin and hepatic and pancreatic enzymes in 30 chronic male alcoholics admitted into psychiatric hospital for detoxification and treatment of alcoholism. Aspartate aminotransferase, alanine aminotransferase, gamma-glutamyltransferase, alkaline phosphatase, and total bilirubin were altered, respectively, in 90%, 63%, 87%, 23% and 23% of the cases. None of the indicators of inflammation (lactic dehydrogenase, altered in 16% of the cases; alpha-1 globulin, 24%; alpha-2 globulin, 88%; leucocyte counts, 28% was correlated with alterations of bilirubin or liver enzymes. Lactic dehydrogenase was poorly sensitive for detection of hepatocytic or muscular damage. Alterations of alpha-globulins seemed to have been due more to alcohol metabolism-induced increase of lipoproteins than to inflammation. Among indicators of cell damage, serum iron, increased in 40% of the cases, seemed to be related to liver damage while creatine phosphokinase, increased in 84% of the cases, related to muscle damage. Hyperamylasemia was found in 20% of the cases and significantly correlated with levels of bilirubin, alkaline phosphatase and gamma-glutamyltransferase. It was indicated that injuries of liver, pancreas, salivary glands, and muscle occurred in asymptomatic or oligosymptomatic chronic alcoholics.

  8. 用于清除胆红素的磁性亲和分离方法%Study on Removal of Bilirubin with Magnetic Affinity Separation Technique

    Institute of Scientific and Technical Information of China (English)

    徐辉; 张国亮; 张凤宝; 王淑兰

    2003-01-01

    An affinity adsorbent, Cibacron Blue 3GA immobilized magnetic polyvinyl alcohol (PVA) microsphereswas used for bilirubin removal taking the advantage of easy separation of magnetic sorbent from the biosystem.Fe3 O4 superparamagnetic particles was synthesized with hydrothermal reaction of ferrous chloride (FeCl2) and ferricchloride (FeCl3). Such magnetic particles are then encapsulated in biocompatible PVA to form magnetic polymermicrospheres sized from 2 to 15 nm with hydroxyl groups on its surface. Cibacron Blue 3GA, a dye-ligand, wascovalently coupled with the polyvinyl alcohol through the nucleophilic reaction between the chloride of its triazinering and the hydroxyl groups of PVA molecules under alkaline condition. The affinity adsorbent carried 21.1 μmolCibacron Blue 3GA per gram magnetic polymer microspheres was used to remove unconjugated and conjugatedbilirubin from the solution which was composed of bilirubin or bilirubin and protein. After the adsorption, theadsorbent loaded with bilirubin was removed easily in the magnetic field.

  9. 新生儿胆红素脑病发病机制与临床评价%Pathogenesis and clinical evaluation of bilirubin encephalopathy in newborn infant

    Institute of Scientific and Technical Information of China (English)

    毛健

    2006-01-01

    @@ 新生儿时期严重的高胆红素血症,特别是生后1周内发生的严重高胆红素血症常易导致急性神经系统功能障碍,即急性胆红素脑病(Acute bilirubin encephalopathy,ABE).

  10. Effect of preoperative biliary drainage on surgical results after pancreaticoduodenectomy in patients with distal common bile duct cancer:Focused on the rate of decrease in serum bilirubin

    Institute of Scientific and Technical Information of China (English)

    Yun Mee Choi; Seok-Hwan Shin; Kyung Rae Kim; Ze-Hong Woo; Eung-Ho Cho; Keon-Young Lee; Seung-Ik Ahn; Sun Keun Choi; Sei Joong Kim; Yoon Seok Hut; Young Up Cho; Kee-Chun Hang

    2008-01-01

    AIM:To examine if the rate of decrease in serum bilirubin after preoperative biliary drainagecan be used as a predicting factor for surgical complications and postoperative recovery after pancreaticoduodenectomy in patients with distal common bile duct cancer.METHODS:A retrospective study was performed in 49 consecutive patients who underwent pancreaticoduodenectomy for distal common bile duct cancer.Potential risk factors were compared between the complicated and uncomplicated groups.Also,the rates of decrease in serum bilirubin were compared pre-and postoperatively.RESULTS:Preoperative biliary drainage (PBD) was performed in 40 patients (81.6%).Postoperative morbidity and mortality rates were 46.9% (23/49) and 6.1% (3/49),respectively.The presence or absence of PBD was not different between the complicated and uncomplicated groups.In patients with PBD,neither the absolute level nor the rate of decrease in serum bilirubin was significantly different.Patients with rapid decrease preoperatively showed faster decrease during the first postoperative week (5.5±4.4 μmol/L vs-1.7±9.9μmol/L,P=0.004).CONCLUSION:PBD does not affect the surgical outcome of pancreaticoduodenectomy in patients with distal common bile duct cancer.There is a certain group of patients with a compromised hepatic excretory function,which is represented by the slow rate of decrease in serum bilirubin after PBD.

  11. High sensitive C-reactive protein and serum amyloid A are inversely related to serum bilirubin : effect-modification by metabolic syndrome

    NARCIS (Netherlands)

    Deetman, Petronella E.; Bakker, Stephan J. L.; Dullaart, Robin P. F.

    2013-01-01

    Background: Bilirubin has been implicated in cardiovascular protection by virtue of its anti-inflammatory and anti-oxidative properties. The metabolic syndrome is featured by enhanced low-grade systemic inflammation and oxidative stress. Serum amyloid A (SAA) impairs anti-oxidative properties of hig

  12. 新生儿胆红素脑病脑脊液胆红素及脑干听觉诱发电位检测的临床意义%The clinical significance of cerebrospinal fluid bilirubin and brainstem auditory evoked potential tests in neonatal bilirubin encephalopathy

    Institute of Scientific and Technical Information of China (English)

    李先红; 张健; 查萍; 王丽丽; 孔萤; 孙璐路; 郑洪

    2014-01-01

    Objective To explore the clinical significance of cerebrospinal fluid bilirubin level and brainstem auditory evoked poten -tial in neonatal bilirubin encephalopathy .Methods All the cases with hyperbilirubinemia were selected from July 2011 to July 2013 in our department,and were divided into the bilirubin encephalopathy group (44 cases) and the non-bilirubin encephalopathy group (79 cases).Ce-rebrospinal fluid ,serum bilirubin level and brainstem auditory evoked potential ( BAEP ) changes were compared between the two groups of children .Results The cerebrospinal fluid bilirubin level in bilirubin encephalopathy group was higher than that of non -bilirubin encephalopa-thy group [(11.0 +3.9) tendency/L vs (7.4 +4.0) tendency/L],and the difference was statistically significant (P<0.01).The inci-dence of abnormal BAEP (61.4%) in bilirubin encephalopathy group was higher than that of non-bilirubin encephalopathy group (16.5%), and the difference was statistically significant (P<0.01).Conclusion Cerebrospinal fluid bilirubin can be used as a reliable indicator for bilirubin encephalopathy in the early diagnosis ,and newborn infants with bilirubin encephalopathy accompanied by higher incidence of hearing damage require early intervention treatment .%目的:探讨新生儿胆红素脑病脑脊液胆红素水平及脑干听觉诱发电位(BAEP)检查的临床意义。方法选取高胆红素血症新生儿为研究对象,分为胆红素脑病组(44例)和非胆红素脑病组(79例),比较两组患儿脑脊液、血清胆红素水平以及BAEP变化。结果胆红素脑病组患儿脑脊液胆红素水平高于非胆红素脑病组[(11.0±3.9) mol/L VS (7.4±4.0) mol/L],差异有统计学意义(P<0.05);胆红素脑病组BAEP异常发生率(61.4%)高于非胆红素脑病组(16.5%),差异有统计学意义(P<0.01)。结论脑脊液胆红素水平检测可作为新生儿胆红素脑病早期诊断的可

  13. Co-Electrodeposition of Bilirubin Oxidase with Redox Polymer through Ligand Substitution for Use as an Oxygen Reduction Cathode

    Energy Technology Data Exchange (ETDEWEB)

    Shin, Hyo Sul; Kang, Chan [Chonbuk National University, Jeonju (Korea, Republic of)

    2010-11-15

    The water soluble redox polymer, poly(N-vinylimidazole) complexed with Os(4,4'-dichloro-2,2'-bipyridine){sub 2}Cl]{sup +} (PVI-[Os(dCl-bpy){sub 2}Cl]{sup +}), was electrodeposited on the surface of a glassy carbon electrode by applying cycles of alternating square wave potentials between 0.2 V (2 s) and 0.7 V (2 s) to the electrode in a solution containing the redox polymer. The coordinating anionic ligand, Cl- of the osmium complex, became labile in the reduced state of the complex and was substituted by the imidazole of the PVI chain. The ligand substitution reactions resulted in crosslinking between the PVI chains, which made the redox polymer water insoluble and caused it to be deposited on the electrode surface. The deposited film was still electrically conducting and the continuous electrodeposition of the redox polymer was possible. When cycles of square wave potentials were applied to the electrode in a solution of bilirubin oxidase and the redox polymer, the enzyme was co-electrodeposited with the redox polymer, because the enzymes could be bound to the metal complexes through the ligand exchange reactions. The electrode with the film of the PVI-[Os(dCl-bpy){sub 2}Cl]{sup +} redox polymer and the co-electrodeposited bilirubin oxidase was employed for the reduction of O{sub 2} and a large increase of the currents was observed due to the electrocatalytic O{sub 2} reduction with a half wave potential at 0.42 V vs. Ag/AgCl.

  14. Bilirubin scavenges chloramines and inhibits myeloperoxidase-induced protein/lipid oxidation in physiologically relevant hyperbilirubinemic serum.

    Science.gov (United States)

    Boon, A C; Hawkins, C L; Coombes, J S; Wagner, K H; Bulmer, A C

    2015-09-01

    Hypochlorous acid (HOCl), an oxidant produced by myeloperoxidase (MPO), induces protein and lipid oxidation, which is implicated in the pathogenesis of atherosclerosis. Individuals with mildly elevated bilirubin concentrations (i.e., Gilbert syndrome; GS) are protected from atherosclerosis, cardiovascular disease, and related mortality. We aimed to investigate whether exogenous/endogenous unconjugated bilirubin (UCB), at physiological concentrations, can protect proteins/lipids from oxidation induced by reagent and enzymatically generated HOCl. Serum/plasma samples supplemented with exogenous UCB (≤250µM) were assessed for their susceptibility to HOCl and MPO/H2O2/Cl(-) oxidation, by measuring chloramine, protein carbonyl, and malondialdehyde (MDA) formation. Serum/plasma samples from hyperbilirubinemic Gunn rats and humans with GS were also exposed to MPO/H2O2/Cl(-) to: (1) validate in vitro data and (2) determine the relevance of endogenously elevated UCB in preventing protein and lipid oxidation. Exogenous UCB dose-dependently (P<0.05) inhibited HOCl and MPO/H2O2/Cl(-)-induced chloramine formation. Albumin-bound UCB efficiently and specifically (3.9-125µM; P<0.05) scavenged taurine, glycine, and N-α-acetyllysine chloramines. These results were translated into Gunn rat and GS serum/plasma, which showed significantly (P<0.01) reduced chloramine formation after MPO-induced oxidation. Protein carbonyl and MDA formation was also reduced after MPO oxidation in plasma supplemented with UCB (P<0.05; 25 and 50µM, respectively). Significant inhibition of protein and lipid oxidation was demonstrated within the physiological range of UCB, providing a hypothetical link to protection from atherosclerosis in hyperbilirubinemic individuals. These data demonstrate a novel and physiologically relevant mechanism whereby UCB could inhibit protein and lipid modification by quenching chloramines induced by MPO-induced HOCl.

  15. 血清胆红素与代谢综合征的相关性研究%Relationship Between Serum Bilirubin and Metabolic Syndrome

    Institute of Scientific and Technical Information of China (English)

    韩硕; 张壬; 金元哲

    2015-01-01

    Objective] To explore the correlation between serum bilirubin and metabolic syndrome (MS) .[Methods] A total of 2 ,125 participants in our survey of atherosclerosis and related diseases were recruited to complete questionnaire ,physical examinations and laboratory tests .They were divided into metabolic syndrome (MS) and non‐metabolic syndrome (NMS) groups according to the International Diabetes Federation (IDF) diag‐nostic criteria for MS .And the correlations were analyzed for MS and serum total bilirubin ,direct bilirubin and in‐direct bilirubin .[Results] Their age range was 35~64 years .With an overall incidence rate of MS of 36 .1% ,it was higher in females than males (43 .5% vs 18 .4% ,P <0 .01) .Direct bilirubin was higher in MS group than that in NMS group [(2 .07 ± 0 .86) vs (2 .29 ± 0 .96)μmol/L ,P <0 .01] .With a rising number of MS composi‐tion factors ,bilirubin levels decreased .Among MS individuals ,serum total bilirubin and direct bilirubin levels were higher in males than those in females ( P<0 .05) .Logistic regression analysis showed that a low direct bili‐rubin level was more susceptible to MS .[Conclusion]Serum bilirubin may be associated with MS and direct biliru‐bin has greater correlations with MS .Individuals with lower serum direct bilirubin levels are more susceptible to MS .%【目的】研究血清胆红素与代谢综合征(MS)的相关性。【方法】对参与辽宁省沈阳市城区动脉硬化及其相关疾病调查的居民进行问卷调查、体格检查和实验室检查,依据国际糖尿病联盟诊断标准将其分为MS组及非代谢综合征(NMS)组。分析受试者血清总胆红素(TBIL)、直接胆红素(DBIL)及间接胆红素(IBIL)与MS的相关性。【结果】共纳入2125名35~64周岁居民,MS的总发生率为36.1%,女性高于男性(43.5%vs18.4%,P<0.01)。MS组DBIL浓度低于NMS组[(2.07±0.86)μmol/Lvs(2.29±0.96

  16. Relationship between serum bilirubin and diabetic kideny disease in patients with type 2 diabetes%2型糖尿病患者血清胆红素水平与糖尿病肾病关系的研究

    Institute of Scientific and Technical Information of China (English)

    陈宇; 周永华; 韩晓骏; 严冲; 苏如婷; 李小飞

    2011-01-01

    目的 探讨2型糖尿病(type 2 diabetes mellitus,T2DM)患者血清胆红素水平与糖尿病肾病(diabetickidney disease,DKD)之间的关系.方法 789例T2DM患者,按照24小时尿白蛋白定量分为正常尿白蛋白组(尿白蛋白300 mg),并以168例正常成人作为对照组,观察各组之间血清胆红素的差异,探讨血清胆红素水平与DKD的关系.结果 T2DM患者血清总胆红素、直接胆红素和间接胆红素水平均低于正常对照组(P0.05);临床尿白蛋白组的总胆红素、直接胆红素以及间接胆红素均低于其他两组(P0.05;r=-0.106,P0.05;r=0.12,P<0.05.结论 胆红素可能是T2DM患者肾脏的保护性因子.糖尿病早期采取积极的抗氧化治疗对防止DKD的发生发展有重要意义.%Objective To approach the relationship between serum bilirubin level and diabetic kideny disease in patients with type 2 diabetes mellitus(T2DM).Methods 789 T2DM patients were classified into normoalbuminuria group (urinary albumin excretion less than 30 mg per 24 h), microalbuminuria group (urinary albumin excretion range from 30 to 300 mg per 24 h) and macroalbuminuria group (urinary albumin excretion more than 300 mg per 24 h) according to the degree of urinary albumin excretion.We chose 168 healthy adults randomly as the control group.The difference in serum bilirubin among groups and the correlation between serum bilirubin and diabetic nephropathy were analyzed.Results T2DM group had higher levels of serum total bilirubin, direct bilirubin and unconjugated bilirubin significantly than those of the control group ( P <0.01 or P <0.05).The total bilirubin and unconjugated bilirubin levels of the microalbuminuria group were lower compared with those of the normoalbuminuria group ( P <0.01, P < 0.05), while there was no significant difference in the direct bilirubin (P >0.05).Compared with the other two groups, the macroalbuminuria group had lower serum total bilirubin, direct bilirubin as well as

  17. Influence of curcumin on cyclosporin-induced reduction of biliary bilirubin and cholesterol excretion and on biliary excretion of cyclosporin and its metabolites.

    Science.gov (United States)

    Deters, M; Siegers, C; Hänsel, W; Schneider, K P; Hennighausen, G

    2000-06-01

    We investigated the ability of curcumin, which can be extracted from different Curcuma species, to prevent cyclosporin-induced reduction of biliary bilirubin and cholesterol excretion, and its influence on biliary excretion of cyclosporin (CS) and its metabolites in the bile fistula model in rats. I.v. injection of curcumin (25 and 50 mg/kg) after 30 min increased dose-dependently basal bile flow (30 microliters/kg/min) up to 200%, biliary bilirubin excretion (3000 pmol/kg/min) up to 150%, and biliary cholesterol excretion (22 nmol/kg/min) up to 113%. CS (30 mg/kg) reduced bile flow to 66% and biliary excretion of bilirubin and of cholesterol to 33% of the basal value 30 min after i.v. injection. I.v. administration of curcumin (25 and 50 mg/kg) 30 min after CS increased bile flow dose dependently again to 130% for 1 hour and biliary excretion of cholesterol and of bilirubin to 100% of the basal value for 30 and 150 min, respectively. Injection of curcumin 15 min before CS prevented the CS-induced drop of bile flow at 50 mg/kg and reduction of biliary bilirubin excretion already at 25 mg/kg until the end of the experiment (180 min). The CS-induced reduction of biliary cholesterol excretion, however, was not prevented by curcumin. Finally, the biliary excretions of CS (1200 ng/kg/min) and its metabolites (1200 ng/kg/min) were slightly reduced by curcumin at a dose of 50 mg/kg (to 83% of the initial values). The clinical importance of these controversial effects remains to be shown.

  18. 胆红素对凝血功能检测结果的影响%Influence of bilirubin on the coagulation function test results

    Institute of Scientific and Technical Information of China (English)

    刘淑芹

    2015-01-01

    目的:探讨不同胆红素浓度对血浆凝血酶原时间(PT)和活化部分凝血活酶时间(APTT)检测结果的影响.方法:对含有不同总胆红素浓度的质控血浆进行血浆凝血酶原时间和活化部分凝血活酶时间指标测定,比较不同浓度的胆红素对上述指标的影响.结果:随着血浆总胆红素浓度的升高,PT、APTT检测结果升高,胆红素水平低于20 μmol/L时,对血浆PT、APTT测定结果影响的差异无统计学意义(P>0.05),当胆红素浓度高于41 μmol/L时,APTT检测结果显著高于对照血浆水平(P0.05).When the bilirubin concentration was more than 41 μmol/L,the APTT test result was significantly higher than the controlled plasma level(P<0.05).When the bilirubin concentration was more than 62 μ mol/L,the PT test result was significantly higher than the controlled plasma level(P<0.05).Conclusion:When the bilirubin level is less than 20 μ mol/L,the influence on the coagulation function test results is not big.However the plasma bilirubin concentration is significantly increased,it has distraction on the PT and APTT test results.When simply because of elevated bilirubin levels lead to PT, APTT results abnormal increase,it could be used the regression equation for correction to provide more accurate detection results in clinic.

  19. Conjugated bilirubin affects cytokine profiles in hepatitis A virus infection by modulating function of signal transducer and activator of transcription factors.

    Science.gov (United States)

    Castro-García, Flor P; Corral-Jara, Karla F; Escobedo-Melendez, Griselda; Sandoval-Hernandez, Monserrat A; Rosenstein, Yvonne; Roman, Sonia; Panduro, Arturo; Fierro, Nora A

    2014-12-01

    Hepatitis A virus (HAV) infection is the major cause of acute liver failure in paediatric patients. The clinical spectrum of infection is variable, and liver injury is determined by altered hepatic enzyme function and bilirubin concentration. We recently reported differences in cytokine profiles between distinct HAV-induced clinical courses, and bilirubin has been recognized as a potential immune-modulator. However, how bilirubin may affect cytokine profiles underlying the variability in the course of infection has not been determined. Herein, we used a transcription factor (TF) binding site identification approach to retrospectively analyse cytokine expression in HAV-infected children and to predict the entire set of TFs associated with the expression of specific cytokine profiles. The results suggested that modulation of the activity of signal transducers and activators of transcription proteins (STATs) may play a central role during HAV infection. This led us to compare the degree of STAT phosphorylation in peripheral blood lymphoid cells (PBLCs) from paediatric patients with distinct levels of conjugated bilirubin (CB). Low CB levels in sera were associated with increased STAT-1 and STAT-5 phosphorylation. A positive correlation was observed between the serum interleukin-6 (IL-6) content and CB values, whereas higher levels of CB correlated with reduced serum IL-8 values and with a reduction in the proportion of PBLCs positive for STAT-5 phosphorylation. When CB was used to stimulate patients' PBLCs in vitro, the levels of IL-6 and tumour necrosis factor-α were increased. The data showed that bilirubin plays a role in STAT function and affects cytokine profile expression during HAV infection.

  20. Developmental onset of bilirubin-induced neurotoxicity involves Toll-like receptor 2-dependent signaling in humanized UDP-glucuronosyltransferase1 mice.

    Science.gov (United States)

    Yueh, Mei-Fei; Chen, Shujuan; Nguyen, Nghia; Tukey, Robert H

    2014-02-21

    Biological and signaling events that connect developmentally induced hyperbilirubinemia to bilirubin-induced neurological dysfunction (BIND) and CNS toxicity in humans are poorly understood. In mammals, UDP-glucuronosyltransferase 1A1 (UGT1A1) is the sole enzyme responsible for bilirubin glucuronidation, a rate-limiting step necessary for bilirubin metabolism and clearance. Humanized mice that express the entire UGT1 locus (hUGT1) and the UGT1A1 gene, develop neonatal hyperbilirubinemia, with 8-10% of hUGT1 mice succumbing to CNS damage, a phenotype that is presented by uncontrollable seizures. We demonstrate that neuroinflammation and reactive gliosis are prominent features of bilirubin brain toxicity, and a disturbed redox status resulting from activation of NADPH oxidase is an important contributing mechanism found in BIND. Using knock-out mice and primary brain cells, we connect a key pattern recognition receptor, Toll-like receptor 2 (TLR2), to hyperbilirubinemia-induced signaling. We illustrate a requirement for TLR2 signaling in regulating gliosis, proinflammatory mediators, and oxidative stress when neonatal mice encounter severe hyperbilirubinemia. TLR2-mediated gliosis strongly correlates with pronounced neuroinflammation in the CNS with up-regulation of TNFα, IL-1β, and IL-6, creating a pro-inflammatory CNS environment. Gene expression and immunohistochemistry staining show that hUGT1/Tlr2(-/-) mice fail to activate glial cells, proinflammatory cytokines, and stress response genes. In addition, bilirubin-induced apoptosis was significantly enhanced by blocking TLR2 signaling indicating its anti-apoptotic property. Consequently, a higher neonatal death rate (57.1%) in hUGT1/Tlr2(-/-) mice was observed when compared with hUGT1 mice (8.7%). These results suggest that TLR2 signaling and microglia neuroinflammation are linked to a repair and/or protection mode against BIND.

  1. 新生儿胆红素脑病33例临床分析%A retrospective study of neonatal severe bilirubin encephalopathy in 33 newborns

    Institute of Scientific and Technical Information of China (English)

    徐瑞峰; 吴珠明; 高红霞; 易彬

    2011-01-01

    目的 探讨新生儿胆红素脑病的病因及临床特点.方法 选择2006年1月至2010年1月我院新生儿科收治的重症新生儿高胆红素血症患儿,分为胆红素脑病组(脑病组)与非胆红素脑病组(非脑病组),比较两组患儿病因、临床特征、胆红素水平、胆红素/白蛋白比值(B/A)以及治疗转归.结果 脑病组黄疸病因以溶血性因素占首位(48.5%),其次是感染因素(24.2%);非脑病组黄疸病因主要为溶血(69.7%).脑病组总胆红素、B/A比值、入院日龄和黄疸持续时间均大于非脑病组[( 555.2±113.9) μmol/L比(431.3±62.3)μmol/L,(0.87±0.17)比(0.67±0.11),(129.5±60.7)h比(53.0±22.6)h,(81.6±39.6)h比(34.2±15.8)h,P均<0.001].胆红素脑病警告期与痉挛期患儿入院日龄、血清胆红素及B/A比值差异无统计学意义(P>0.05).85%的胆红素脑病患儿预后不良.结论 溶血与感染是新生儿胆红素脑病的主要原因,总胆红素浓度过高和干预延迟是引起胆红素脑病的高危因素.%Objective To investigate the etiology and clinical features of neonatal bilirubin encephalopathy. Methods To select severe hyperbilirubinemia infant admitted in Gansu Provincial Maternity and Children's Hospital from January 2006 to January 2010, assigned into encephalopathy group and non-encephalopathy group, comparing their clinical characteristics, bilirubin level, bilirubin/ albumin ratio (B/A) and outcome. Results The main etiology of neonatal bilirubin encephalopathy is hemolytic (48. 5% ) , followed by infection (24. 2% ) ; but the etiology of non-encephalopathy is mainly for hemolysis, accounted for 69. 7%. Total bilirubin, B/A ratio, admission day and jaundice duration of Encephalopathy group are larger than them in non-encephalopathy group, the difference was statistically significant, (555. 2 ±113. 9) μmol/Lvs. (431. 3 ±62. 3) |xmol/L, (0.87 ±0.17) us. (0.67 ±0. 11) , (129.5 ±60.7) h ts. (53.0 ±22.6) h and (81.6 ±39.6) h

  2. 新生儿胆红素脑病脑脊液总胆红素及颅脑MRI检查的临床意义%Clinical Significance of Cerebrospinal Fluid Bilirubin and Craniocerebral Magnetic Resonance Imaging Measurements in Newborns with Bilirubin Encephalopathy

    Institute of Scientific and Technical Information of China (English)

    李先红; 张健; 郑洪; 孔萤; 孙路璐; 刘光辉

    2014-01-01

    Objective To explore the clinical significance of changes in cerebrospinal fluid bilirubin levels and craniocerebral magnetic resonance imaging (MRI)features in newborns with bilir-ubin encephalopathy. Methods Total bilirubin levels in serum and cerebrospinal fluid were determined using vanadate oxidation method with automatic biochemical analyzer in 44 newborns with bilirubin encephalopathy (bilirubin encephalopathy group)and 79 newborns without bilirubin enceph-alopathy (non-bilirubin encephalopathy group).In addition,craniocerebral MRI was performed in both groups. Results Total bilirubin levels in serum and cerebrospinal fluid in bilirubin encephalopathy group were significantly higher than those in non-bilirubin encephalopathy group (P<0.01).In bilirubin enceph-alopathy group,MRI showed symmetrical high signal intensity in the globus pallidus on T1WI in 33 newborns,abnormal signal in lateral side of posterior limb of internal capsule in the right basal ganglion region in 1 newborn,high signal intensity in the head of the right caudate nucleus on T1WI in 1 newborn, abnormal signal in the lateral ventricle in 3 newborns,abnormal signal in the bilateral frontal lobes in 1 newborn, short T1T2 signal in the torcular herophili in 1 newborn,and normal signal in 4 newborns. In non-bilirubin encephalopathy group,MRI showed symmetrical high signal intensity in the globus pallidus on T1WI in 3 newborns, abnormal signal in the right side of top occipital in 1 newborn, and normal signal in 75 newborns. The incidence of symmetrical high T1WI signal intensity in the globus pallidus in bilirubin encephalopathy group was significantly higher than that in non-bilirubin encephalopathy group (P<0.01).Conclusion Cerebrospinal fluid bilirubin can be used as a reliable indicator for the early diagnosis of bilirubin encephalopathy. The symmetrical high signal intensity in the globus pallidus on T1WI is the main feature of bilirubin encephalopathy in newborns. Regular fol-low-up and

  3. INVESTIGATION ON ASSOCIATION OF SERUM BILIRUBIN CONCENTRATION WITH RISK OF CORONARY HEART DISEASE%血清胆红素与冠心病的关系探讨

    Institute of Scientific and Technical Information of China (English)

    张书亚; 陈红涛

    2001-01-01

    Objective  To investigate the relationship between of serum bilirubin concentrations and risk of coronary heart drsease (CHD). Methods  Serum bilirubin concentrations were determined in 12 cases with acute myocardial infarction(AMI)、37 cases with unstable angina pectoris (UAP) and 30 cases with UAP with arrhythmia. Results  The serum total bilirubin(TB)、direct bilirubin(DB)、indirect bilirubin(IB) concentrations were lower in the three study groups of CHD than in the controls. There was significant difference between the patients with CHD and the controls. However, there was no significant difference among the three study groups of CHD. Conclusion  Serum bilirubin concentrations was inversely and statistically significantly related to risk of CHD.%目的探讨血清胆红素浓度与冠心病(CHD)发生的危险性之间的关系。方法观察79例CHD患者血清胆红素浓度,再据冠心病分型将其分为急性心肌梗死(AMI)组12例,不稳定型心绞痛(UAP)组37例,不稳定型心绞痛并心律失常组30例。同时观察30例健康体检组血清胆红素浓度。结果冠心病组及不同类型冠心病组血清总胆红素、直接胆红素、间接胆红素浓度均降低,与健康对照组比较均有显著性差异,而不同类型冠心病组间比较差异无显著性。结论血清胆红素浓度与冠心病发生的危险性之间呈显著的负相关。

  4. Avaliação laboratorial da estabilidade do padrão calibrador de bilirrubina Laboratorial evaluation of standard bilirubin stability

    Directory of Open Access Journals (Sweden)

    Maria das Graças da Cunha Leite

    2003-01-01

    Full Text Available Introdução: O preparo do padrão calibrador de bilirrubina é essencial para um controle adequado das dosagens laboratoriais da bilirrubinemia, visto que estas estão sujeitas a grande variabilidade nos resultados, dependendo do método de dosagem escolhido e da falta de padronização rigorosa na sua execução. Uma vez preparado, este padrão calibrador deve ser dividido em alíquotas e estocado para ser utilizado de rotina. Objetivo: Avaliar os efeitos de diferentes condições de armazenamento de um padrão calibrador de bilirrubina sobre sua estabilidade, com finalidade de calibração de equipamentos utilizados na determinação da bilirrubinemia em neonatos. Material e métodos: Após o preparo de um padrão calibrador com 25mg/dl de bilirrubina, este foi armazenado a 4°C, congelado a - 20°C e a - 70°C. Durante nove meses foram feitas dosagens consecutivas da bilirrubina da solução padrão, as quais foram analisadas através da análise de variância de duas vias com blocagem. Resultados: As amostras congeladas a - 70°C não sofreram degradação significativa nos nove meses estudados, enquanto que, no período de três meses, as congeladas a - 20°C e a 4°C sofreram uma degradação de 5% e 24,18%, respectivamente, dos níveis iniciais de bilirrubina. Conclusão: A estocagem do padrão calibrador de bilirrubina a - 70°C é a recomendada para a preservação dos níveis de bilirrubina.Background: The preparation of a standard bilirubin is essential for an adequate quality control of laboratorial bilirubinemia measurements because they are subjected to a large variability in results depending on the dosage method used and the lack of a rigorous standardization of its performance. Once prepared, this standard solution has to be divided in aliquots and stored to be routinely used. Objective: To evaluate the effect of different conditions of the standard solution storage in the stability of bilirubin with the purpose of using it for

  5. Electrochemical characterization of adsorbed bilirubin oxidase on Vulcan XC 72R for the biocathode preparation in a glucose/O{sub 2} biofuel cell

    Energy Technology Data Exchange (ETDEWEB)

    Habrioux, A.; Napporn, T.; Servat, K. [LACCO ' Equipe Electrocatalyse' UMR 6503 CNRS-Universite de Poitiers, 40 av. du Recteur Pineau 86022 Poitiers (France); Tingry, S. [Institut Europeen des Membranes, UMR 5635, Place Eugene Bataillon, CC 047, 34095 Montpellier, cedex 5 (France); Kokoh, K.B., E-mail: boniface.kokoh@univ-poitiers.f [LACCO ' Equipe Electrocatalyse' UMR 6503 CNRS-Universite de Poitiers, 40 av. du Recteur Pineau 86022 Poitiers (France)

    2010-11-01

    A new biocathode was built and tested. It consisted of bilirubin oxidase adsorbed on Vulcan XC 72 R and immobilized into a Nafion matrix. The possibility of direct electron transfer between bilirubin oxidase and Vulcan XC 72 R was also demonstrated. The kinetics on biocathode were enhanced by including 2,2'-azinobis-3-ethylbenzothiazoline-5-sulfonic acid in the catalytic film. A first order reaction rate was observed for oxygen concentrations lower than 22%. A complete kinetic investigation of the system was shown. A biofuel cell test performed with this biocathode and Au{sub 70}Pt{sub 30} nanoparticles as anode catalyst permitted to reach a power density of 170 {mu}W cm{sup -2} at a cell voltage of 0.6 V, which is superior to what can be obtained with the concentric design.

  6. Anti-cancer effects of blue-green alga Spirulina platensis, a natural source of bilirubin-like tetrapyrrolic compounds.

    Science.gov (United States)

    Koníčková, Renata; Vaňková, Kateřina; Vaníková, Jana; Váňová, Kateřina; Muchová, Lucie; Subhanová, Iva; Zadinová, Marie; Zelenka, Jaroslav; Dvořák, Aleš; Kolář, Michal; Strnad, Hynek; Rimpelová, Silvie; Ruml, Tomáš; J Wong, Ronald; Vítek, Libor

    2014-01-01

    Spirulina platensis is a blue-green alga used as a dietary supplement because of its hypocholesterolemic properties. Among other bioactive substances, it is also rich in tetrapyrrolic compounds closely related to bilirubin molecule, a potent antioxidant and anti-proliferative agent. The aim of our study was to evaluate possible anticancer effects of S. platensis and S. platensis-derived tetrapyrroles using an experimental model of pancreatic cancer. The anti-proliferative effects of S. platensis and its tetrapyrrolic components [phycocyanobilin (PCB) and chlorophyllin, a surrogate molecule for chlorophyll A] were tested on several human pancreatic cancer cell lines and xenotransplanted nude mice. The effects of experimental therapeutics on mitochondrial reactive oxygen species (ROS) production and glutathione redox status were also evaluated. Compared to untreated cells, experimental therapeutics significantly decreased proliferation of human pancreatic cancer cell lines in vitro in a dose-dependent manner (from 0.16 g•L-1 [S. platensis], 60 μM [PCB], and 125 μM [chlorophyllin], palga. Furthermore, it seems that dietary supplementation with this alga might enhance systemic pool of tetrapyrroles, known to be higher in subjects with Gilbert syndrome.

  7. Research Update: Facile synthesis of CoFe2O4 nano-hollow spheres for efficient bilirubin adsorption

    Science.gov (United States)

    Rakshit, Rupali; Pal, Monalisa; Chaudhuri, Arka; Mandal, Madhuri; Mandal, Kalyan

    2015-11-01

    Herein, we report an unprecedented bilirubin (BR) adsorption efficiency of CoFe2O4 (CFO) nanostructures in contrast to the commercially available activated carbon and resin which are generally used for haemoperfusion and haemodialysis. We have synthesized CFO nanoparticles of diameter 100 nm and a series of nano-hollow spheres of diameter 100, 160, 250, and 350 nm using a simple template free solvothermal technique through proper variation of reaction time and capping agent, oleylamine (OLA), respectively, and carried out SiO2 coating by employing Stöber method. The comparative BR adsorption study of CFO and SiO2 coated CFO nanostructures indicates that apart from porosity and hollow configuration of nanostructures, the electrostatic affinity between anionic carboxyl group of BR and cationic amine group of OLA plays a significant role in adsorbing BR. Finally, we demonstrate that the BR adsorption capacity of the nanostructures can be tailored by varying the morphology as well as size of the nanostructures. We believe that our developed magnetic nanostructures could be considered as a potential material towards therapeutic applications against hyperbilirubinemia.

  8. Decolorization of anthraquinone-type dye by bilirubin oxidase-producing nonligninolytic fungus Myrothecium sp. IMER1.

    Science.gov (United States)

    Zhang, Xiaoyu; Liu, Youxun; Yan, Keliang; Wu, Hangjun

    2007-08-01

    The decolorization of an anthraquinone dye, Remazol Brilliant Blue R (RBBR), was carried out using a new isolated nonligninolytic fungus, strain Myrothecium sp. IMER1. In potato dextrose broth (PDB) containing RBBR, this strain was able to grow and decolorize the dye efficiently at pHs ranging from 4.0 to 9.0, and the optimal pH and temperature were pH 7.0 and 28 degrees C. A decolorization efficiency of approximately 90% was achieved by cultivation for 7 d at an initial dye concentration of 80 mg l(-1). The adsorption of the dye by cells was observed at the beginning of the decolorization, then the color became faint and finally disappeared when bilirubin oxidase (BOX) was released by the strain. Additionally, the visual observation and ultraviolet- visible (UV-VIS) spectral analysis demonstrated that decolorization involved biosorption and biodegradation. Native polyacrylamide gel electrophoresis of crude enzyme and purified BOX confirmed that BOX, which is an important extracellular oxidoreductase, played a major role in decolorization. Furthermore, purified BOX was demonstrated to degrade RBBR and other dyes by in vitro enzymatic experiments. Our results suggest that both the strain and its extracellular BOX have promising applications in dye effluent decolorization.

  9. Bilirubin oxidase-like proteins from Podospora anserina: promising thermostable enzymes for application in transformation of plant biomass.

    Science.gov (United States)

    Xie, Ning; Ruprich-Robert, Gwenaël; Silar, Philippe; Chapeland-Leclerc, Florence

    2015-03-01

    Plant biomass degradation by fungi is a critical step for production of biofuels, and laccases are common ligninolytic enzymes envisioned for ligninolysis. Bilirubin oxidases (BODs)-like are related to laccases, but their roles during lignocellulose degradation have not yet been fully investigated. The two BODs of the ascomycete fungus Podospora anserina were characterized by targeted gene deletions. Enzymatic assay revealed that the bod1(Δ) and bod2(Δ) mutants lost partly a thermostable laccase activity. A triple mutant inactivated for bod1, bod2 and mco, a previously investigated multicopper oxidase gene distantly related to laccases, had no thermostable laccase activity. The pattern of fruiting body production in the bod1(Δ) bod2(Δ) double mutant was changed. The bod1(Δ) and bod2(Δ) mutants were reduced in their ability to grow on ligneous and cellulosic materials. Furthermore, bod1(Δ) and bod2(Δ) mutants were defective towards resistance to phenolic substrates and H2 O2 , which may also impact lignocellulose breakdown. Double and triple mutants were more affected than single mutants, evidencing redundancy of function among BODs and mco. Overall, the data show that bod1, bod2 and mco code for non-canonical thermostable laccases that participate in the degradation of lignocellulose. Thanks to their thermal stability, these enzymes may be more promising candidate for biotechnological application than canonical laccases.

  10. Evaluation of treatment thresholds for unconjugated hyperbilirubinemia in preterm infants: effects on serum bilirubin and on hearing loss?

    Directory of Open Access Journals (Sweden)

    Christian V Hulzebos

    Full Text Available BACKGROUND: Severe unconjugated hyperbilirubinemia may cause deafness. In the Netherlands, 25% lower total serum bilirubin (TSB treatment thresholds were recently implemented for preterm infants. OBJECTIVE: To determine the rate of hearing loss in jaundiced preterms treated at high or at low TSB thresholds. DESIGN/METHODS: In this retrospective study conducted at two neonatal intensive care units in the Netherlands, we included preterms (gestational age 35 dB. RESULTS: There were 479 patients in the high and 144 in the low threshold group. Both groups had similar gestational ages (29.5 weeks and birth weights (1300 g. Mean and mean peak TSB levels were significantly lower after the implementation of the novel thresholds: 152 ± 43 µmol/L and 212 ± 52 µmol/L versus 131 ± 37 µmol/L and 188 ± 46 µmol/L for the high versus low thresholds, respectively (P<0.001. The incidence of hearing loss was 2.7% (13/479 in the high and 0.7% (1/144 in the low TSB threshold group (NNT = 50, 95% CI, 25-3302. CONCLUSIONS: Implementation of lower treatment thresholds resulted in reduced mean and peak TSB levels. The incidence of hearing impairment in preterms with a gestational age <32 weeks treated at low TSB thresholds was substantially lower compared to preterms treated at high TSB thresholds. Further research with larger sample sizes and power is needed to determine if this effect is statistically significant.

  11. Modulation of defect-mediated energy transfer from ZnO nanoparticles for the photocatalytic degradation of bilirubin

    Directory of Open Access Journals (Sweden)

    Tanujjal Bora

    2013-11-01

    Full Text Available In recent years, nanotechnology has gained significant interest for applications in the medical field. In this regard, a utilization of the ZnO nanoparticles for the efficient degradation of bilirubin (BR through photocatalysis was explored. BR is a water insoluble byproduct of the heme catabolism that can cause jaundice when its excretion is impaired. The photocatalytic degradation of BR activated by ZnO nanoparticles through a non-radiative energy transfer pathway can be influenced by the surface defect-states (mainly the oxygen vacancies of the catalyst nanoparticles. These were modulated by applying a simple annealing in an oxygen-rich atmosphere. The mechanism of the energy transfer process between the ZnO nanoparticles and the BR molecules adsorbed at the surface was studied by using steady-state and picosecond-resolved fluorescence spectroscopy. A correlation of photocatalytic degradation and time-correlated single photon counting studies revealed that the defect-engineered ZnO nanoparticles that were obtained through post-annealing treatments led to an efficient decomposition of BR molecules that was enabled by Förster resonance energy transfer.

  12. An ethanol/O{sub 2} biofuel cell based on an electropolymerized bilirubin oxidase/Pt nanoparticle bioelectrocatalytic O{sub 2}-reduction cathode

    Energy Technology Data Exchange (ETDEWEB)

    Yan, Yi-Ming; Baravik, Ilina; Tel-Vered, Ran; Willner, Itamar [Institute of Chemistry, Hebrew University of Jerusalem (Israel)

    2009-11-13

    An effective O{sub 2}-reducing bioelectrocatalytic electrode is prepared by the electrochemical crosslinking of thioaniline-modified Pt nanoparticles (NPs) and thioaniline-functionalized bilirubin oxidase (BOD). An O{sub 2}/ethanol biofuel cell element is constructed by integrating the Pt NP/BOD cathode and an electrically contacted alcohol dehydrogenase (AlcDH)-based anode. (Abstract Copyright [2009], Wiley Periodicals, Inc.)

  13. Phenobarbital and Phototherapy Combination Enhances Decline of Total Serum Bilirubin and May Decrease the Need for Blood Exchange Transfusion in Newborns with Isoimmune Hemolytic Disease

    Science.gov (United States)

    Kaabneh, Mahmoud AF; Salama, Ghassan SA; Shakkoury, Ayoub GA; Al-abdallah, Ibrahim MH; Alshamari, Afrah; Halaseh, Ruba AA

    2015-01-01

    OBJECTIVE The objective of this study was to evaluate the effect of phenobarbital and phototherapy combination on the total serum bilirubin of the newborn infants with isoimmune hemolytic disease (IHD) and its impact on blood exchange transfusion rates. PATIENTS AND METHOD This single-blinded, prospective, randomized, controlled trial was conducted between March 2013 and December 2014 at the pediatric ward of two Military Hospitals in Jordan. A total of 200 full-term neonates with IHD were divided randomly into two groups: (1) the phenobarbital plus phototherapy group (n = 103), and (2) the phototherapy-only group (n = 97). Infants in group 1 received an oral dose of 2.5 mg/kg phenobarbital every 12 hours for 3 days in addition to phototherapy. The total serum bilirubin was observed. RESULTS Of the total 200 included newborn infants, 186 infants completed the study: 97 infants were included in group 1 and 89 infants in group 2. The difference between the mean total serum bilirubin levels at 24, 48, and 72 hours after starting the trial was clinically and statistically significant at P < 0.05. The differences between the two groups were also statistically significant at P < 0.05. Of the total 186 who completed the study, only 22 underwent blood exchange transfusion [7 from group 1, and 15 from group 2 (P = 0.0478)]. CONCLUSION In a limited-resources setting, phenobarbital in combination with phototherapy may be helpful to newborn infants with IHD, as it results in a faster decline in total serum bilirubin, thus decreasing the need for blood exchange transfusion than phototherapy alone. PMID:26309423

  14. Acute effect of weight loss on levels of total bilirubin in obese, cardiovascular high-risk patients: an analysis from the lead-in period of the Sibutramine Cardiovascular Outcome trial

    DEFF Research Database (Denmark)

    Andersson, Charlotte; Weeke, Peter; Fosbøl, Emil Loldrup;

    2009-01-01

    Low levels of bilirubin are associated with an increased risk of cardiovascular adverse events. Weight reduction is known to reduce several cardiovascular risk factors, but effects on bilirubin levels have not been reported. We studied the response of weight loss therapy with sibutramine...... and lifestyle change on levels of total bilirubin in an overweight or obese, cardiovascular high-risk population. Data from the first 4 weeks of the lead-in period of the Sibutramine Cardiovascular Outcome study were analyzed. A total of 10 198 patients provided body weight measurements before and after 4 weeks......, respectively. At screening, bilirubin concentrations were similar between weight loss groups (around 11 micromol/L, P = .7) and increased linearly as a function of weight loss. The effect was significantly more pronounced in men compared with women (P for interaction = .003). Adjusted for multiple variables...

  15. 原发性肝癌切除术后高胆红素血症临床分析%Clinical investigation on postoperative high serum bilirubin in patients with primary liver cancer after resection

    Institute of Scientific and Technical Information of China (English)

    黄涛; 周进学; 杨楠木; 宫东伟

    2011-01-01

    目的 探讨原发性肝癌切除术后胆红素变化的规律及导致高胆红素血症的临床因素.方法 回顾性分析97例原发性肝癌切除术患者临床资料,根据术后血清总胆红素水平分为高胆红素组和胆红素正常组,观察术后胆红素变化特点,分析导致术后高胆红素血症的临床因素.结果 红素正常组术后血清总胆红素4 d达峰值[(21.5±9.2)μmol/L],术后14 d可降至正常水平;高胆红素血症组血清总胆红素7 d达峰值[(49.2±25.4)μmol/L],术后14 d仍高于正常值2倍[(36.1 ±17.6)μmol/L].单因素Logistic分析显示:术前胆红素水平,肝功能Child分级,术中肝血流阻断方式,出血量,输血情况以及术后合并低钠血症与术后高胆红素血症有关;多因素Logistic回归分析结果 表明术前胆红素水平是原发性肝癌切除术后高胆红素血症独立预测指标(OR=5.406,χ2=11.319,P=0.001).结论 原发性肝癌切除术后动态监测血清胆红素水平是重要的.围手术期对发生商胆红素血症的相关临床因素积极防范并处理,可降低肝癌切除术后并发症发生率.%Objective To investigate the features of postoperative serum bilirubin changes and the clinical factors associated with high serum bilirubin level in patients with primary liver cancer after resection. Methods The clinical data of 97 patients with primary liver cancer who underwent hepatectomy were analysed retrospectively. The cases were divided into two groups: high serum bilirubin group and normal bilirubin group, respectively, according to serum bilirubin level during two weeks after operation. The features of postoperative serum bilirubin changes and the causitive factors of postoperative high serum bilirubin were analyzed. Results The postoperative serum bilirubin reached the peak value [ (21. 5 ± 9.2 )μmol/L ] on d4, and then decreased to normal on d 14 in normal bilirubin group. But in high serum bilirubin group the postoperative serum

  16. Research on Fetal Bilirubin Metabolism and the Relationship Between It and Fetal Outcome%胎儿胆红素代谢及其与胎儿结局的关系

    Institute of Scientific and Technical Information of China (English)

    王晓璐

    2011-01-01

    胆红素代谢对人体有重要作用,游离型胆红素可自由穿过脂膜和血脑屏障等,产生细胞毒性而引发新生儿核黄疸.胎儿胆红素代谢与成人不同,胎儿期肝脏尚未发育成熟,其体内只有少量胆红素可经肝脏转化为无毒的结合型胆红素,大部分是非结合型胆红素以胆红素-白蛋白复合物的形式溶于血浆中,通过胎盘上的胆红素转运载体运送至母体代谢.当胎儿体内胆红素浓度过高或白蛋白浓度降低等病理情况下,胆红素可从白蛋白结合位点上解离成为游离型胆红素,其可对胎儿产生神经毒性作用,造成胎儿不良结局.%Bilirubin metabolism plays an important role for the human,free bilirubin can pass through cell membranes, blood-brain barrier and lipid membranes freely, resulting in cell toxicity and neonatal kernicterus.Fetal bilirubin metabolism is different from the adults'. Because of the immaturity of fetal liver function, only a small amount of fetal bilirubin can be transformed into non-toxic conjugated bilirubin via fetal liver, while most of the unconjugated bilirubin dissolved in the plasma in the form of bilirubin-albumin complex is transferred to mother to be metabolized by bilirubin carriers in the placenta. In certain pathological conditions, such as high concentration of bilirubin or low concentration of albumin, bilirubin-albumin complex may be dissociated from albumin binding sites to free bilirubin, which can result in neurotoxic effects on fetus, causing fetal adverse outcomes.

  17. A needle extraction utilizing a molecularly imprinted-sol-gel xerogel for on-line microextraction of the lung cancer biomarker bilirubin from plasma and urine samples.

    Science.gov (United States)

    Moein, Mohammad Mahdi; Jabbar, Dunia; Colmsjö, Anders; Abdel-Rehim, Mohamed

    2014-10-31

    In the present work, a needle trap utilizing a molecularly imprinted sol-gel xerogel was prepared for the on-line microextraction of bilirubin from plasma and urine samples. Each prepared needle could be used for approximately one hundred extractions before it was discarded. Imprinted and non-imprinted sol-gel xerogel were applied for the extraction of bilirubin from plasma and urine samples. The produced molecularly imprinted sol-gel xerogel polymer showed high binding capacity and fast adsorption/desorption kinetics for bilirubin in plasma and urine samples. The adsorption capacity of molecularly imprinted sol-gel xerogel polymer was approximately 60% higher than that of non-imprinted polymer. The effect of the conditioning, washing and elution solvents, pH, extraction time, adsorption capacity and imprinting factor were investigated. The limit of detection and the lower limit of quantification were set to 1.6 and 5nmolL(-1), respectively using plasma or urine samples. The standard calibration curves were obtained within the concentration range of 5-1000nmolL(-1) in both plasma and urine samples. The coefficients of determination values (R(2)) were ≥0.998 for all runs. The extraction recovery was approximately 80% for BR in the human plasma and urine samples.

  18. Functional induction of the cystine-glutamate exchanger system Xc(- activity in SH-SY5Y cells by unconjugated bilirubin.

    Directory of Open Access Journals (Sweden)

    Pablo J Giraudi

    Full Text Available We have previously reported that exposure of SH-SY5Y neuroblastoma cells to unconjugated bilirubin (UCB resulted in a marked up-regulation of the mRNA encoding for the Na(+-independent cystine∶glutamate exchanger System X(c(- (SLC7A11 and SLC3A2 genes. In this study we demonstrate that SH-SY5Y cells treated with UCB showed a higher cystine uptake due to a significant and specific increase in the activity of System X(c(-, without the contribution of the others two cystine transporters (X(AG(- and GGT reported in neurons. The total intracellular glutathione content was 2 folds higher in the cells exposed to bilirubin as compared to controls, suggesting that the internalized cystine is used for gluthathione synthesis. Interestingly, these cells were significantly less sensitive to an oxidative insult induced by hydrogen peroxide. If System X(c(- is silenced the protection is lost. In conclusion, these results suggest that bilirubin can modulate the gluthathione levels in neuroblastoma cells through the induction of the System X(c(-, and this renders the cell less prone to oxidative damage.

  19. Study on dioxygen reduction by mutational modifications of the hydrogen bond network leading from bulk water to the trinuclear copper center in bilirubin oxidase

    Energy Technology Data Exchange (ETDEWEB)

    Morishita, Hirotoshi; Kurita, Daisuke; Kataoka, Kunishige; Sakurai, Takeshi, E-mail: tsakurai@se.kanazawa-u.ac.jp

    2014-07-18

    Highlights: • Proton transport pathway in bilirubin oxidase was mutated. • Two intermediates in the dioxygen reduction steps were trapped and characterized. • A specific glutamate for dioxygen reduction by multicopper oxidases was identified. - Abstract: The hydrogen bond network leading from bulk water to the trinuclear copper center in bilirubin oxidase is constructed with Glu463 and water molecules to transport protons for the four-electron reduction of dioxygen. Substitutions of Glu463 with Gln or Ala were attributed to virtually complete loss or significant reduction in enzymatic activities due to an inhibition of the proton transfer steps to dioxygen. The single turnover reaction of the Glu463Gln mutant afforded the highly magnetically interacted intermediate II (native intermediate) with a broad g = 1.96 electron paramagnetic resonance signal detectable at cryogenic temperatures. Reactions of the double mutants, Cys457Ser/Glu463Gln and Cys457Ser/Glu463Ala afforded the intermediate I (peroxide intermediate) because the type I copper center to donate the fourth electron to dioxygen was vacant in addition to the interference of proton transport due to the mutation at Glu463. The intermediate I gave no electron paramagnetic resonance signal, but the type II copper signal became detectable with the decay of the intermediate I. Structural and functional similarities between multicopper oxidases are discussed based on the present mutation at Glu463 in bilirubin oxidase.

  20. Bilirubin inhibits the up-regulation of inducible nitric oxide synthase by scavenging reactive oxygen species generated by the toll-like receptor 4-dependent activation of NADPH oxidase.

    Science.gov (United States)

    Idelman, Gila; Smith, Darcey L H; Zucker, Stephen D

    2015-08-01

    It has been previously shown that bilirubin prevents the up-regulation of inducible nitric oxide synthase (iNOS) in response to LPS. The present study examines whether this effect is exerted through modulation of Toll-Like Receptor-4 (TLR4) signaling. LPS-stimulated iNOS and NADPH oxidase (Nox) activity in RAW 264.7 murine macrophages was assessed by measuring cellular nitrate and superoxide ( [Formula: see text] ) production, respectively. The generation of both nitrate and [Formula: see text] in response to LPS was suppressed by TLR4 inhibitors, indicating that activation of iNOS and Nox is TLR4-dependent. While treatment with superoxide dismutase (SOD) and bilirubin effectively abolished LPS-mediated [Formula: see text] production, hydrogen peroxide and nitrate release were inhibited by bilirubin and PEG-catalase, but not SOD, supporting that iNOS activation is primarily dependent upon intracellular H2O2. LPS treatment increased nuclear translocation of the redox-sensitive transcription factor Hypoxia Inducible Factor-1α (HIF-1α), an effect that was abolished by bilirubin. Cells transfected with murine iNOS reporter constructs in which the HIF-1α-specific hypoxia response element was disrupted exhibited a blunted response to LPS, supporting that HIF-1α mediates Nox-dependent iNOS expression. Bilirubin, but not SOD, blocked the cellular production of interferon-β, while interleukin-6 production remained unaffected. These data support that bilirubin inhibits the TLR4-mediated up-regulation of iNOS by preventing activation of HIF-1α through scavenging of Nox-derived reactive oxygen species. Bilirubin also suppresses interferon-β release via a ROS-independent mechanism. These findings characterize potential mechanisms for the anti-inflammatory effects of bilirubin.

  1. 新生儿经皮胆红素与血清胆红素测定影响因素及高胆红素血症感染的临床分析%Factors influencing determination of neonatal transcutaneous bilirubin and serum bilirubin and clinical analysis of hyperbilirubinemia

    Institute of Scientific and Technical Information of China (English)

    金霆; 费政芳; 严争

    2013-01-01

    OBJECTIVE To explore the related factors influencing the determination of the neonatal transcutaneous bilirubin and serum bilirubin and to analyze the causes of the neonatal infection hyperbilirubinemia. METHODS The transcutaneous bilirubin value in 250 children with hyperbilirubinemia and the bilirubin value of the bypass peripheral blood and serum bilirubin value were compared and analyzed, then the predisposing factors were analyzed. RESULTS The determination values of the transcutaneous bilirubin, peripheral blood bilirubin, and total serum bilirubin were(145.6±23. 5)mol/L, (148.3±20. l)mol/L, and (155. 2±15. 2) μmol/L, respectively, the differences were statistically significant(P<0. 001 ). There were 107 (42. 8%) cases of neonates who caught the neonatal hyperbilirubinemia primarily due to the perinatal factors, 81 (32. 4%) cases due to the infection factors. The infection factors mainly consisted of omphalitis, impetigo, septicemia, pneumonia, purulent meningitis, toxoplas-mosis infection, giant CMV infection, and congenital syphilis. CONCLUSION The determination of transcutaneous bilirubin is highly correlated with the level of serum bilirubin. The infection factors take a secondary place to cause the hyperbilirubinemia, the treatment should be conducted according to the specific cause by taking different treatment programs.%目的 探讨新生儿经皮胆红素与血清胆红素测定的影响因素及高胆红素血症感染因素的临床分析.方法 对发生胆红素血症250例患儿经皮胆红素值与经末梢血和血清测定的总胆红素值进行对照,同时对患儿的发病因素进行分析.结果 婴儿经皮胆红素与末梢血胆红素以及血清总胆红素测定值分别为(145.6±23.5)、(148.3±20.1)、(155.2±15.2)μmol/L,差异有统计学意义(P<0.01);导致新生儿高胆红素血症的最主要因素为围产因素107例,占42.8%,感染因素81例,占32.4%,感染因素主要包括脐炎、脓疱疹、败血症

  2. Study on the quality control of bilirubin in self-made serum%自制血清总胆红素质控品研究

    Institute of Scientific and Technical Information of China (English)

    牟虹; 李强

    2009-01-01

    目的 自制胆红素室内质控品作为测定总胆红素(TBIL)参比血清,评价自制室内质控品是否符合临床要求,降低检验成本.方法 收集日常工作中TBIL高、中、低值标本分别混合后分装并加入赋形剂,经低温干燥至干粉,低温避光冷藏,即为自制质控品.结果 自制室内质控品在26 d内与新鲜混合血清的TBIL比较,差异无统计学意义(P>0.05).结论 自制TBIL室内质控品符合室内质控要求,该分装方法用于自制室内质控品,可降低检验成本.%Objective Using bilirubin self-made laboratory quality control of products as a total bilirubin (TB) Reference Serum to evaluate the self-made laboratory quality control about whether to fit the clinical requirements and to reduce the cost of testing.Methods Collect the high,medium,low and mixed loaded total bilirubin(TB) respectively at the routine working days, and then add some excipients and put it in the place under low temperature till dry powder, which should be stored in cold 、dark and frozen environment.Results Within 26 days, compared the self-made laboratory quality control products(frozen mixed serum) with the fresh mixed blood plasma total bilirubin (TB),the difference was not significant (P> 0.05).Conclusion Total bilirubin self-made laboratory quality control of products meet with the requirements, so this sub-loaded method can be used for quality control laboratory of self-made products, and reduce testing costs.

  3. Influence of Phosphatidylcholine and Calcium on Self-Association and Bile Salt Mixed Micellar Binding of the Natural Bile Pigment, Bilirubin Ditaurate.

    Science.gov (United States)

    Neubrand, Michael W; Carey, Martin C; Laue, Thomas M

    2015-11-17

    Recently [Neubrand, M. W., et al. (2015) Biochemistry 54, 1542-1557], we determined a concentration-dependent monomer-dimer-tetramer equilibrium in aqueous bilirubin ditaurate (BDT) solutions and explored the nature of high-affinity binding of BDT monomers with monomers and micelles of the common taurine-conjugated bile salts (BS). We now investigate, employing complementary physicochemical methods, including fluorescence emission spectrophotometry and quasi-elastic light scattering spectroscopy, the influence of phosphatidylcholine (PC), the predominant phospholipid of bile and calcium, the major divalent biliary cation, on these self-interactions and heterointeractions. We have used short-chain, lyso and long-chain PC species as models and contrasted our results with those of parallel studies employing unconjugated bilirubin (UCB) as the fully charged dianion. Both bile pigments interacted with the zwitterionic headgroup of short-chain lecithins, forming water-soluble (BDT) and insoluble ion-pair complexes (UCB), respectively. Upon micelle formation, BDT monomers apparently remained at the headgroup mantle of short-chain PCs, but the ion pairs with UCB became internalized within the micelle's hydrophobic core. BDT interacted with the headgroups of unilamellar egg yolk (EY) PC vesicles; however, with the simultaneous addition of CaCl2, a reversible aggregation took place, but not vesicle fusion. With mixed EYPC/BS micelles, BDT became bound to the hydrophilic surface (as with simple BS micelles), and in turn, both BDT and BS bound calcium, but not other divalent cations. The calcium complexation of BDT and BS was enhanced strongly with increases in micellar EYPC, suggesting calcium-mediated cross-bridging of hydrophilic headgroups at the micelle's surface. Therefore, the physicochemical binding of BDT to BS in an artificial bile medium is influenced not only by BS species and concentration but also by long-chain PCs and calcium ions that exert a specific rather

  4. Bilirubin UDP-Glucuronosyltransferase 1A1 (UGT1A1) Gene Promoter Polymorphisms and HPRT, Glycophorin A, and Micronuclei Mutant Frequencies in Human Blood

    Energy Technology Data Exchange (ETDEWEB)

    Grant, D; Hall, I J; Eastmond, D; Jones, I M; Bell, D A

    2004-10-06

    A dinucleotide repeat polymorphism (5-, 6-, 7-, or 8-TA units) has been identified within the promoter region of UDP-glucuronosyltransferase 1A1 gene (UGT1A1). The 7-TA repeat allele has been associated with elevated serum bilirubin levels that cause a mild hyperbilirubinemia (Gilbert's syndrome). Studies suggest that promoter transcriptional activity of UGT1A1 is inversely related to the number of TA repeats and that unconjugated bilirubin concentration increases directly with the number of TA repeat elements. Because bilirubin is a known antioxidant, we hypothesized that UGT1A1 repeats associated with higher bilirubin may be protective against oxidative damage. We examined the effect of UGT1A1 genotype on somatic mutant frequency in the hypoxanthine-guanine phosphoribosyl-transferase (HPRT) gene in human lymphocytes and the glycophorin A (GPA) gene of red blood cells (both N0, NN mutants), and the frequency of lymphocyte micronuclei (both kinetochore (K) positive or micronuclei K negative) in 101 healthy smoking and nonsmoking individuals. As hypothesized, genotypes containing 7-TA and 8-TA displayed marginally lower GPA{_}NN mutant frequency relative to 5/5, 5/6, 6/6 genotypes (p<0.05). In contrast, our analysis showed that lower expressing UGT1A1 alleles (7-TA and 8-TA) were associated with modestly increased HPRT mutation frequency (p<0.05) while the same low expression genotypes were not significantly associated with micronuclei frequencies (K-positive or K-negative) when compared to high expression genotypes (5-TA and 6-TA). We found weak evidence that UGT1A1 genotypes containing 7-TA and 8-TA were associated with increased GPA{_}N0 mutant frequency relative to 5/5, 5/6, 6/6 genotypes (p<0.05). These data suggest that UGT1A1 genotype may modulate somatic mutation of some types, in some cell lineages, by a mechanism not involving bilirubin antioxidant activity. More detailed studies examining UGT1A1 promoter variation, oxidant/antioxidant balance and

  5. Exposure to lipopolysaccharide and/or unconjugated bilirubin impair the integrity and function of brain microvascular endothelial cells.

    Directory of Open Access Journals (Sweden)

    Filipa L Cardoso

    Full Text Available BACKGROUND: Sepsis and jaundice are common conditions in newborns that can lead to brain damage. Though lipopolysaccharide (LPS is known to alter the integrity of the blood-brain barrier (BBB, little is known on the effects of unconjugated bilirubin (UCB and even less on the joint effects of UCB and LPS on brain microvascular endothelial cells (BMEC. METHODOLOGY/PRINCIPAL FINDINGS: Monolayers of primary rat BMEC were treated with 1 µg/ml LPS and/or 50 µM UCB, in the presence of 100 µM human serum albumin, for 4 or 24 h. Co-cultures of BMEC with astroglial cells, a more complex BBB model, were used in selected experiments. LPS led to apoptosis and UCB induced both apoptotic and necrotic-like cell death. LPS and UCB led to inhibition of P-glycoprotein and activation of matrix metalloproteinases-2 and -9 in mono-cultures. Transmission electron microscopy evidenced apoptotic bodies, as well as damaged mitochondria and rough endoplasmic reticulum in BMEC by either insult. Shorter cell contacts and increased caveolae-like invaginations were noticeable in LPS-treated cells and loss of intercellular junctions was observed upon treatment with UCB. Both compounds triggered impairment of endothelial permeability and transendothelial electrical resistance both in mono- and co-cultures. The functional changes were confirmed by alterations in immunostaining for junctional proteins β-catenin, ZO-1 and claudin-5. Enlargement of intercellular spaces, and redistribution of junctional proteins were found in BMEC after exposure to LPS and UCB. CONCLUSIONS: LPS and/or UCB exert direct toxic effects on BMEC, with distinct temporal profiles and mechanisms of action. Therefore, the impairment of brain endothelial integrity upon exposure to these neurotoxins may favor their access to the brain, thus increasing the risk of injury and requiring adequate clinical management of sepsis and jaundice in the neonatal period.

  6. 血清胆红素与老年冠心病的相关性探讨%Correlation study of serum bilirubin and coronary heart disease in elderly patients

    Institute of Scientific and Technical Information of China (English)

    颜承靖; 颜群

    2013-01-01

    目的 探讨血清胆红素与老年冠心病的相关性. 方法 选取本院冠心病患者37例和体检健康老年者54例.记录所有研究对象基本资料,检测血清胆红素、血脂、肝功能,并进行相关分析.结果 冠心病组的血清总胆红素(TBIL)和间接胆红素(IBIL)浓度显著低于非冠心病组(P<0.05),而直接胆红素(DBIL)的水平在2组之间无统计学差异. 结论 在老年冠心病患者中血清胆红素(TBIL、IBIL)水平降低,在生理范围内轻度升高的胆红素是冠心痛的一个保护因素.%Objective To investigate the relationship between serum bilirubin and coronary heart disease in elderly patients. Methods Thirty-seven patients with coronary heart disease and 54 healthy cases were enrolled in this study. The clinical data of all the subjects were recorded. The levels of serum bilirubin,blood lipid,liver function were analyzed. Results The levels of serum total bilirubin (TBIL) and indirect bilirubin (IBIL) in the patients with coronary heart disease were significantly lower than those in the healthy cases (P <0. 05). The level of direct bilirubin ( DBIL) showed no difference between the two groups. Conclusions The level of serum bilirubin are decreased in elderly patients with coronary artery disease. In the physiological range mildly elevated bilirubin is one of the protective factors to coronary heart disease.

  7. Cruciferous vegetable feeding alters UGT1A1 activity: diet- and genotype-dependent changes in serum bilirubin in a controlled feeding trial.

    Science.gov (United States)

    Navarro, Sandi L; Peterson, Sabrina; Chen, Chu; Makar, Karen W; Schwarz, Yvonne; King, Irena B; Li, Shuying S; Li, Lin; Kestin, Mark; Lampe, Johanna W

    2009-04-01

    Chemoprevention by isothiocyanates from cruciferous vegetables occurs partly through up-regulation of phase II conjugating enzymes, such as UDP-glucuronosyltransferases (UGT). UGT1A1 glucuronidates bilirubin, estrogens, and several dietary carcinogens. The UGT1A1*28 polymorphism reduces transcription compared with the wild-type, resulting in decreased enzyme activity. Isothiocyanates are metabolized by glutathione S-transferases (GST); variants may alter isothiocyanate clearance such that response to crucifers may vary by genotype. We evaluated, in a randomized, controlled, crossover feeding trial in humans (n = 70), three test diets (single- and double-"dose" cruciferous and cruciferous plus apiaceous) compared with a fruit and vegetable-free basal diet. We measured serum bilirubin concentrations on days 0, 7, 11, and 14 of each 2-week feeding period to monitor UGT1A1 activity and determined effects of UGT1A1*28 and GSTM1/GSTT1-null variants on response. Aggregate bilirubin response to all vegetable-containing diets was statistically significantly lower compared with the basal diet (P cruciferous diets compared with basal (P cruciferous and cruciferous plus apiaceous compared with basal, and cruciferous plus apiaceous compared with single-dose cruciferous (P vegetable-containing diets compared with basal (P < 0.02 for all). Evaluation of the effects of diet stratified by GST genotype revealed some statistically significant genotypic differences; however, the magnitude was similar and not statistically significant between genotypes. These results may have implications for altering carcinogen metabolism through dietary intervention, particularly among UGT1A1*28/*28 individuals.

  8. 高血压患者血清胆红素与脉搏波速度相关性研究%Relationship between Serum Bilirubin and Pulse Wave Velocity in Hypertensive Patients

    Institute of Scientific and Technical Information of China (English)

    黎兰芳

    2013-01-01

    目的 探讨高血压患者血清胆红素与脉搏波速度的关系.方法 应用康普乐全自动动脉硬化检测系统检测508例确诊为原发性高血压患者的颈-股动脉脉搏波速度(CF-PWV),并检测空腹血清总胆红素.将总胆红素>11 μmol·L-1者作为高胆红素组(n= 221),总胆红素≤11μmol·L-1者作为低胆红素组(n=287),对血清总胆红素与CF-PWV值进行相关性分析.结果 高胆红素组CF-PWV值为(9.04士1.20)m·s-1低于低胆红素组的(10.89±2.18)m·s-1,2组比较差异有统计学意义(P<0.01);血清总胆红素与CF-PWV值呈负相关(r=-0.636,P<0.01).结论 高血压患者血清总胆红素与脉搏波速度呈负相关.%Objective To study the relationship between serum bilirubin and pulse wave velocity (PWV) in hypertensive patients.Methods Carotid-femoral PWV(CF-PWV) was determined by using Complior SP automatic arteriosclerosis measurement system in 508 patients with essential hypertension.According to the fasting serum total bilirubin levels,patients were divided into high bilirubin group (>11 μmol · L-1,n=221) and low bilirubin group (≤11 μmol · L-1,n=287).The correlation between serum total bilirubin and CF-PWV was analyzed.Results Compared with low bilirubin group,CF-PWV significantly decreased in high bilirubin group [(9.04±1.20)m · s-1 vs (10.89±2.18)m · s-1,P<0.01].There was a negative correlation between serum total bilirubin levels and CF-PWV (r=-0.636,P<0.01).Conclusion Serum total bilirubin levels are negatively correlated with PWV in hypertensive patients.

  9. Clinical application value of bilirubin detection in cerebrospinal fluid of neonates with hyperbilirubinemia%高胆红素血症新生儿脑脊液胆红素检测的临床应用价值探讨

    Institute of Scientific and Technical Information of China (English)

    周柏林; 王全震; 吕清秀; 董静

    2013-01-01

    Objective:To explore the clinical application value of unconjugated bilirubin detection in cerebrospinal fluid of neonates with hyperbilirubinemia.Methods:The levels of unconjugated bilirubin in cerebrospinal fluid and blood of 285 neonates with hyperbilirubinemia were detected,the ratio of unconjugated bilirubin to albumin (B/A) was calculated,and neonatal behavioral neurological assessment (NBNA) was performed.Results:The levels of unconjugated bilirubin in blood and cerebrospinal fluid,B/A in blood in neonates with bilirubin encephalopathy at warning period and spasm period were statistically significantly higher than those in neonates with simple hyperbilirubinemia (P < 0.05),the level of unconjugated bilirubin in cerebrospinal fluid of neonates with bilirubin encephalopathy at spasm period was statistically significantly higher than that of neonates with bilirubin encephalopathy at warning period (P < 0.05),the levels of unconjugated bilirubin in blood and cerebrospinal fluid,B/A in cerebrospinal fluid of neonates with abnormal NBNA score were statistically significantly higher than those of neonates with normal NBNA score,there was a significant correlation between unconjugated bilirubin and NBNA score in cerebrospinal fluid.Conclusion:Unconjugated bilirubin detection in cerebrospinal fluid not only diagnose bilirubin encephalopathy early,but also reflect the brain function of neonates with hyperbilirubinemia.%目的:探讨脑脊液中未结合胆红素检测在高胆红素血症新生儿中的临床应用价值.方法:对285例高胆红素血症新生儿的脑脊液和血液未结合胆红素水平进行检测,计算未结合胆红素/白蛋白比值(B/A),并进行新生儿行为神经评分(NBNA).结果:警告期和痉挛期的胆红素脑病新生儿血中未结合胆红素(UCB)、脑脊液(CSF)中UCB和血B/A比值均明显高于单纯高胆红素血症的新生儿,差异有统计学意义(P<0.05),并且出现痉挛期的新生儿CSF中UCB高于警

  10. Study on the risk factors and preventive measures of neonatal bilirubin encephalopathy%新生儿胆红素脑病危险因素与预防措施研究

    Institute of Scientific and Technical Information of China (English)

    吴俊超; 刘军民; 刘华勇

    2011-01-01

    目的:探讨导致新生儿胆红索脑病的危险因索,优化其关键性预防措施.方法:回顾性分析1998年1月~2007年12月4 683例新生儿高间接胆红素血症(HB)及其中64例并发胆红素脑病(BE)的临床资料.结果:①4 683例HB患儿中的甲组2 366例为该院分娩,接受胆红素动态监测,HB得到及时治疗,无BE发生;乙组2 317例为市、县、乡镇分娩,没有动态监测胆红素并及时治疗HB,并发64例BE.甲乙两组病因分布对比,差异无统计学意义(P>0.05);②乙组2 317例中,1 187例接受过围产期保健服务者,并发16例BE,1 130例未接受服务者,并发48例BE,对比检验,差异有统计学意义(P<0.01);③导致64例BE的危险因素为:一是围产期母儿因素、溶血因素,感染因素等多病因并存,二是存在缺氧、酸中毒、低体温、低蛋白、低血糖、低热量等合并症.结论:建立健全对HB发生、发展的动态监测机制,针对病因,及早治疗HB,防治合并症,是预防或消除BE的关键措施;加强围产期保键服务,也能有效地降低BE发生率.%Objective: To explore the risk factors of neonatal bilirubin encephalopathy, and optimize its key preventive measures. Methods: The clinical data of 4 683 neonates with indirect hyperbilirubinemia and 64 neonates with indirect hyperbilirubinemia combined with bilirubin encephalopathy from January 1998 to December 2007 were analyzed retrospectively. Results: Among 4 683 neonates with indirect hyperbilirubinemia, 2 366 neonates in group A deliveried in the hospital received dynamic monitoring of bilirubin and they were treated timely, no bilirubin encephalopathy occurred; 2 317 neonates in group B deliveried in hospitals in other cities, counties and townships didn't receive dynamic monitoring of bilirubin and they were not treated timely, 64 neonates combined with bilirubin encephalopathy. There was no significant difference in the distribution of etiological causes between the two groups

  11. Simple and sensitive method for the quantification of total bilirubin in human serum using 3-methyl-2-benzothiazolinone hydrazone hydrochloride as a chromogenic probe

    Science.gov (United States)

    Nagaraja, Padmarajaiah; Avinash, Krishnegowda; Shivakumar, Anantharaman; Dinesh, Rangappa; Shrestha, Ashwinee Kumar

    2010-11-01

    We here describe a new spectrophotometric method for measuring total bilirubin in serum. The method is based on the cleavage of bilirubin giving formaldehyde which further reacts with diazotized 3-methyl-2-benzothiazolinone hydrazone hydrochloride giving blue colored solution with maximum absorbance at 630 nm. Sensitivity of the developed method was compared with Jendrassik-Grof assay procedure and its applicability has been tested with human serum samples. Good correlation was attained between both methods giving slope of 0.994, intercept 0.015, and R2 = 0.997. Beers law obeyed in the range of 0.068-17.2 μM with good linearity, absorbance y = 0.044 Cbil + 0.003. Relative standard deviation was 0.006872, within day precision ranged 0.3-1.2% and day-to-day precision ranged 1-6%. Recovery of the method varied from 97 to 102%. The proposed method has higher sensitivity with less interference. The obtained product was extracted and was spectrally characterized for structural confirmation with FT-IR, 1H NMR.

  12. Preparation and surface properties of mesoporous silica particles modified with poly(N-vinyl-2-pyrrolidone) as a potential adsorbent for bilirubin removal

    Energy Technology Data Exchange (ETDEWEB)

    Timin, Alexander, E-mail: a_timin@mail.ru [Inorganic Chemistry Department, Ivanovo State University of Chemistry and Technology (ISUCT), 7, Sheremetevsky prosp., 153000 Ivanovo (Russian Federation); Rumyantsev, Evgeniy, E-mail: evr@isuct.ru [Inorganic Chemistry Department, Ivanovo State University of Chemistry and Technology (ISUCT), 7, Sheremetevsky prosp., 153000 Ivanovo (Russian Federation); Lanin, Sergey N., E-mail: SNLanin@phys.chem.msu.ru [Chemistry Department, Physical Chemistry Division, Lomonosov Moscow State University, 1-3 Leninskie Gory, 119991 Moscow (Russian Federation); Rychkova, Sveta A. [Chemistry Department, Physical Chemistry Division, Lomonosov Moscow State University, 1-3 Leninskie Gory, 119991 Moscow (Russian Federation); Guseynov, Sabir S. [Krestov Institute of Solution Chemistry of Russian Academy of Sciences, 153000 Ivanovo (Russian Federation); Solomonov, Alexey V. [Inorganic Chemistry Department, Ivanovo State University of Chemistry and Technology (ISUCT), 7, Sheremetevsky prosp., 153000 Ivanovo (Russian Federation); Antina, Elena V. [Krestov Institute of Solution Chemistry of Russian Academy of Sciences, 153000 Ivanovo (Russian Federation)

    2014-10-15

    The surface of silica particles was modified with polyvinyl pyrrolidone (PVP) through sol–gel process. The different experimental techniques, i.e., thermogravimetric analysis (TGA and DTG), nitrogen adsorption, scanning electron microscopy (SEM), laser diffraction analysis (LDA), fourier transform spectroscopy (FTIR) are used to characterize the pure non-functionalized and functionalized silicas containing different amount of PVP. It was shown that PVP-modified silica samples have well developed porous structure; the values of specific surface area for PVP-modified silicas are in the range of 140–264 m{sup 2} g{sup −1}. While the non-functionalized silica shows the low surface area (S{sub BET} = 40 m{sup 2} g{sup −1}). The BJH analysis showed that PVP can be used as an effective agent to increase an average pore size and total pore volume. The results indicate that PVP functionalized silicas show a potential as effective adsorbents for bilirubin removal compared to other available adsorbents. - Highlights: • PVP functionalized silicas were synthesized via sol–gel method. • Modification of silica by PVP leads to the formation of mesoporous structure. • PVP functionalized mesoporous silicas demonstrate good adsorption properties for bilirubin removal.

  13. 胆红素与糖尿病慢性并发症相关性研究%Studies on the relation between bilirubin and chronic diabetic complications

    Institute of Scientific and Technical Information of China (English)

    杨艳霞; 苏青

    2016-01-01

    糖尿病心脑血管并发症、糖尿病肾病、糖尿病视网膜病分别是目前糖尿病患者死亡、终末期肾病、成人致盲的主要原因。目前,关于糖尿病并发症的发病机制的研究主要集中于氧化应激与慢性炎症方面。此外,已有大量研究指出胆红素具有抗氧化及抗炎症作用。因此,胆红素与糖尿病并发症有着密切联系。%[Summary] Cardiovascular disease ,diabetic nephropathy and diabetic retinopathy are common complications of diabetes ,and are the significant reason of cardiovascular mortality ,end-stage renal disease ,and blindness in adults patients with type 2 diabetes mellitus. At present, the researche on the pathogenesis of diabetic complications mainly focus on oxidative stress and chronic inflammation. In addition, a large number of studies have pointed out that bilirubin has antioxidant and anti-inflammatory effects. Therefore, bilirubin and diabetic complications are closely linked.

  14. Evaluation of new oxidation methods for the measurement of bilirubin on the aeroset clinical chemistry analyzer and comparison with methods on the Hitachi 717.

    Science.gov (United States)

    Sturm, Ernhard; Albrecht-Groos, Ragnhild; Seyfarth, Michael

    2002-01-01

    We evaluated analytical and performance quality of the new oxidation methods for direct and total bilirubin on the Abbott Aeroset clinical chemistry analyzer. Within-day imprecisions for Abbott Aeroset assays ranged from 0.7 to 2.9% and between-day imprecisions from 2.1 to 7.3%. Inaccuracies as compared with the control "target values" for the Jendrassik-Gróf method showed deviations of -18.2 to +4.2%. Limits of detection were determined and showed very low values of 300 micromol/l. A method comparison for 100 patient samples with established Jendrassik-Gróf and DPD methods on the Roche Hitachi 717 showed good linearities between the investigated methods (r > or = 0.995). Due to slopes that ranged from 0.829 to 0.950, reference ranges for the oxidation methods differ slightly from those of established Roche Jendrassik-Gróf methods, but results can be adapted by the introduction of converting factors. In conclusion, the oxidation bilirubin assays revealed convincing analytical and performance qualities for medical needs that were similar or even better than for established methods. Application of the oxidation methods on the Aeroset clinical chemistry analyzer also improves laboratory efficiency by increasing throughput, speed of obtaining results and lowered sample and reagent volumes compared to established methods.

  15. Study of relationship between the levels of serum bilirubin ,uric acid and unstable angina pectoris%不稳定型心绞痛患者血清胆红素及尿酸水平的分析

    Institute of Scientific and Technical Information of China (English)

    梁启权; 曾胜宏

    2009-01-01

    目的 探讨冠心病不稳定型心绞痛与胆红素及尿酸的关系.方法 将入选202例冠心病不稳定型心绞痛患者,采集空腹静脉血测定总胆红素、直接胆红素、间接胆红素及尿酸,比较其心绞痛发作时、3个月、6个月及1年内未再发作时胆红素及尿酸的变化情况.结果 心绞痛发作时胆红素降低,尿酸偏高.结论 胆红素和尿酸水平可间接反映不稳定型心绞痛机体氧化、抗氧化情况,胆红素对评估病情有一定帮助.%Objective To investigate the relationship between the levels of serum bilirubin,uric acid(UA) and coronary heart disease unstable angina pectoris.Methods The leves of total bilirubin (TBIL),direct bilirubin (DBIL),indirect bilirubin(IBIL) and UA in venous blood by limosis were determind in 202 patients with unstable angina pectoris.We determine the different levels of TBIL,DBIL,IBIL and UA when they were suffering from angina pectoris.Then we respectively determine the levels of TBIL,DBIL,IBIL and UA in 3 months,6 months and 12 months after they had been suffered from angina pectoris.Results The levels of serum bilirubin were lower and UA were higher,when the patients were suffering from angina pectoris.Conclusion We can indirectly assess the oxidative and antioxidative activities by testing bilirubin and UA in unstable angina pectoris.

  16. 糖尿病肾病患者胆红素和血红蛋白的相关性及临床意义%Patients with Diabetic Nephropathy and its Clinical Significance of Bilirubin and Hemoglobin

    Institute of Scientific and Technical Information of China (English)

    尤守堂; 沈红艳

    2015-01-01

    Objective To analyze the relationship between the diabetic nephropathy patients peripheral blood bilirubin and hemoglobin, to explore the factors inlfuencing the serum bilirubin in patients with nephropathy of diabetes. Methods This study included 106 patients with diabetic nephropathy patients, 113 cases of diabetic patients without complications and 120 healthy controls were detected in the peripheral blood, the total bilirubin, direct bilirubin, indirect bilirubin and hemoglobin content and total bilirubin and hemoglobin correlation analysis. Results Compared with the control group and diabetic group without complications, diabetic nephropathy group total bilirubin, direct bilirubin, indirect bilirubin and hemoglobin levels were signiifcantly reduced, the differences were statistically signiifcant (P<0.001);correlation analysis showed, diabetic nephropathy group total bilirubin level was positively associated with hemoglobin (r=0.455, P<0.001). Conclusion Decreased serum bilirubin in patients with diabetic nephropathy associated with the lower hemoglobin, positive correction has an important role for the treatment of anemia in patients with diabetic nephropathy.%目的:分析糖尿病肾病患者外周血胆红素水平与血红蛋白之间的关系,探讨糖尿病肾病患者血清胆红素变化的影响因素。方法研究对象包括106例糖尿病肾病患者,113例糖尿病无并发症患者和120例健康对照者,检测上述各组外周血总胆红素、直接胆红素、间接胆红素和血红蛋白含量并分析总胆红素与血红蛋白相关性。结果与对照组和糖尿病无并发症组比较,糖尿病肾病组总胆红素、直接胆红素、间接胆红素及血红蛋白水平均显著降低,差异均有统计学意义(P<0.001);相关分析显示,糖尿病肾病组总胆红素水平与血红蛋白呈正相关(r=0.455,P<0.001)。结论糖尿病肾病患者血清胆红素的降低与血红蛋白降低有关,积

  17. 急性脑梗死后胆红素升高与卒中严重性的相关研究%Research on Bilirubin increased after AIS associated with the severity of stroke

    Institute of Scientific and Technical Information of China (English)

    张俊玲

    2015-01-01

    Objectives To observe the changes of bilirubin in earlier acute ischemic stroke ( AIS) and whether such changes are associated with the severity of the stroke.Methods Bilirubin and other biochemical indexes were measured in 108 AIS patients and 38 transientischemic attack ( TIA) patients which set as the control group.First, the level of bilirubin and it’s distribution were com-pared between the AIS and control group.According to a cut-off point, we next analyzed the impacted factors of elevated bilirubin in-cluding the direct bilirubin (Dbil) and total bilirubin(Tbil), especially the correlation between elevated bilirubin and the severity of stroke.Results The serum direct bilirubin and total bilirubin in patients with AIS group was significantly higher than the TIA group.In both groups, we found the distribution of intervals is different.The percentage of low bilirubin is lower in AIS group com-pared with TIA group,while the percentage of high bilirubin is higher in AIS group compared with TIA group.Conclusions The ser-um levels of Dbil and Tbil were increased after AIS, which linked to the severity of stroke.%目的:观察急性脑梗死( acute ischemic stroke,AIS)早期胆红素的变化情况及此种改变是否与卒中的严重程度相关。方法选取108例AIS患者作为试验组,38例TIA患者作为对照组,测定所有患者的胆红素以及其他相关的生化指标。首先,比较AIS组与对照组胆红素的水平以及其分布。根据设立的节点,我们分析了胆红素(包括直接胆红素以及总胆红素)升高的影响因素,尤其是分析升高的胆红素与卒中严重程度之间的相关性。结果 AIS组患者血清直接胆红素以及总胆红素显著高于TIA组。在两组中我们发现其分布区间也不同,AIS的低胆红素组比率低于TIA组,而AIS组高胆红素的百分比相比TIA组较高。结论血清直接胆红素和总胆红素水平在脑卒中后升高,并与卒中的严重程度相关。

  18. 2型糖尿病患者血清胆红素水平与糖尿病病程负相关%Increased duration of diabetes is associated with lower serum bilirubin concentration in type 2 diabetic patients

    Institute of Scientific and Technical Information of China (English)

    李佳霖; 陈艳曙; 徐淼; 褚建平; 励丽

    2016-01-01

    Objective To investigate the association between physiological serum bilirubin concentration and duration of type 2 diabetes. Methods A total of 1 272 patients with type 2 diabetes admitted to the Department of Endocrinology of Ningbo First Hospital from January 2013 to June 2015 were included for the study. Clinic parameters were collected from electronic medical records. All subjects were classified into four groups by diabetes duration quartiles (<5 years, 5-9 years, 10-14 years and≥15 years). The adjusted concentration of three types of bilirubin were calculated and compared using covariance analysis. Pearson correlation analysis and multiple linear regression analysis were adopted to examine the relationship between duration of diabetes and serum bilirubin. Results With increase of diabetes duration from the lowest quartiles to the highest quartiles, serum total bilirubin, direct bilirubin and indirect bilirubin concentration tended to decrease. This trend persisted after adjustment for age, gender, hemoglobin, smoking, drinking, blood pressure, lipid profile, weight, ALT, AST, HbA1c and statin use. The adjusted mean concentration of total bilirubin from the lowest quartiles to the highest quartiles for diabetes duration was 13.26, 12.36, 12.07 and 11.71mmol/L (F=4.36, P=0.005). The direct bilirubin was 5.01, 4.67, 4.47 and 4.35mmol/L (F=4.63, P=0.003).The indirect bilirubin was 8.25, 7.69, 7.60 and 7.37 μmol/L(F=2.96, P=0.033). Pearson correlation analysis showed that total bilirubin concentration (r=-0.241, P<0.01), direct bilirubin concentration (r=-0.23,P<0.01), and indirect bilirubin concentration (r=-0.21,P<0.01) were inversely correlated with duration of diabetes. Multiple linear regression analysis showed that an increment of one year in diabetes duration was associated with 0.087mmol/L decrease in total bilirubin, 0.037mmol/L in direct bilirubin and 0.051mmol/L in indirect bilirubin separately. Conclusion Serum bilirubin concentration is negatively

  19. 血清胆红素与尿酸检验对冠心病的诊断价值分析%Diagnostic Value of Serum Bilirubin and Uric Acid in Patients with Coronary Heart Disease

    Institute of Scientific and Technical Information of China (English)

    黄飞

    2016-01-01

    Objective To investigate the role of serum bilirubin and uric acid in the detection of coronary artery disease.Methods Biochemical analysis of 82 cases of various indicators of coronary heart disease patients and healthy people for comparison.Results Bilirubin in observation group was significantly lower than healthy people;higher serum uric acid levels ( <0.05),different groups of serum bilirubin and uric acid levels in patients with coronary heart disease were significantly different ( <0.05).Conclusion Serum bilirubin and uric acid levels in patients with coronary heart disease is detected may be clinically assess the extent of reference.%目的:探讨血清胆红素与尿酸检测对冠心病的作用。方法82例冠心病患者与健康人员对比各生化指标分析。结果观察组胆红素明显低于健康体检者。血清尿酸水平较高(<0.05)﹐不同组别冠心病患者血清胆红素与尿酸水平差异显著(<0.05)。结论冠心病患者血清胆红素与尿酸水平检测可为临床评估病症程度参考。

  20. Bilirubin nomograms for identification of neonatal hyperbilirubinemia in healthy term and late-preterm infants:a systematic review and meta-analysis

    Institute of Scientific and Technical Information of China (English)

    Zhang-Bin Yu; Shu-Ping Han; Chao Chen

    2014-01-01

    Background: Hyperbilirubinemia occurs in most healthy term and late-preterm infants, and must be monitored to identify those who might develop severe hyperbilirubinemia. Total serum bilirubin (TSB) or transcutaneous bilirubin (TcB) nomograms have been developed and validated to identify neonatal hyperbilirubinemia. This study aimed to review previously published studies and compare the TcB nomograms with the TSB nomogram, and to determine if the former has the same predictive value for signifi cant hyperbilirubinemia as TSB nomogram does. Methods: A predefined search strategy and inclusion criteria were set up. We selected studies assessing the predictive ability of TSB/TcB nomograms to identify significant hyperbilirubinemia in healthy term and latepreterm infants. Two independent reviewers assessed the quality and extracted the data from the included studies. Meta-Disc 1.4 analysis software was used to calculate the pooled sensitivity, specificity, and positive likelihood ratio of TcB/TSB nomograms. A pooled summary of the receiver operating characteristic of the TcB/TSB nomograms was created. Results: After screening 187 publications from electronic database searches and reference lists of eligible articles, we included 14 studies in the systematic review and meta-analysis. Eleven studies were of medium methodological quality. The remaining three studies were of low methodological quality. Seven studies evaluated the TcB nomograms, and seven studies assessed TSB nomograms. There were no differences between the predictive abilities of the TSB and TcB nomograms (the pooled area under curve was 0.819 vs. 0.817). Conclusions: This study showed that TcB nomograms had the same predictive value as TSB nomograms, both of which could be used to identify subsequent signifi cant hyperbilirubinemia. But this result should be interpreted cautiously because some methodological limitations of these included studies were identifi ed in this review.

  1. 亲和膜配基的结构和密度对胆红素吸附的影响%Effect of ligand composition and ligand density of affinity membrane on bilirubin removal

    Institute of Scientific and Technical Information of China (English)

    鞠佳; 聂飞; 段志军; 贺高红

    2013-01-01

    High concentration of bilirubin may cause neurotoxicity, permanent brain damage, and even death in severe cases. It is difficult to remove bilirubin from serum by circulated adsorption, because bilirubin can be tightly bound with albumin as a complex in human serum. The albumin in the complex has a larger volume than that of bilirubin, which results in high steric hindrance of adsorption or displacement of bilirubin. In order to enhance serum bilirubin adsorption capacity, five kinds of amines and eight kinds of amino acids as specific ligands were immobilized on cellulose acetate (CA) /polyethyleneimine (PEI) membrane via the glutaraldehyde modification method. Experimental results indicated that although the primary amine contents of modified membranes were only one third of CA/PEI membrane, bilirubin adsorption capacities of four kinds of modified membranes increased by more than 100%. Moreover, the adsorption selectivity of the four modified membranes for bilirubin/human albumin pair was greater than that of CA/ PEI membrane. Prolongation of the spacer and immobilization of specific ligand could be the cause of such results. Ligand composition had a significant influence on bilirubin adsorption capacity. The ligands containing hydrophobic and primary amino groups could enhance bilirubin adsorption capacity, and the ligands with carboxyl radicals could decrease bilirubin adsorption capacity of the modified membrane. Moreover, ligand density, ligand steric hindrance, and other factors could also influence bilirubin adsorption capacity. In the experiments, the ligands containing high steric hindrance groups, such as phenyl groups had a negative effect on bilirubin adsorption capacity of the modified membrane, and its bilirubin adsorption capacity could not be enhanced via increasing ligand density. However, bilirubin adsorption capacity of the membrane modified with low steric hindrance ligand, for example hexamethylene-diamine (3-HMD) -modified membrane

  2. PREPARATION AND ADSORPTION PROPERTIES OF BILIRUBIN ADSORBENTS WITH DIFFERENT LIGANDS BASED ON SEPHAROSE CL-4B%胆红素吸附剂功能基组成及其吸附特性研究

    Institute of Scientific and Technical Information of China (English)

    王智; 陈书博; 徐丽; 谢健; 贾凌云

    2011-01-01

    为了获得性能较佳的胆红素吸附剂,本研究考察了功能基种类、链长、偶联密度等因素对吸附剂吸附性能的影响.结果表明,当功能基具有足够的链长,与胆红素相似的疏水性及较高的偶联密度时,能够提高吸附剂的胆红素吸附能力.实验表明,链长21个原子、偶联73μmol/mL正丁胺的吸附剂对胆红素的吸附能力最强,在30℃、300mg/L的白蛋白结合胆红素溶液中,吸附1h即达到平衡,胆红素吸附量达到3.01 mg/mL (90.3mg/g干重);较高的温度有利于其对胆红素的吸附.50mL材料对500mL实际病人血浆动态吸附2.5h,总胆红素(277.4mg/L)去除率达到55.2%,同时对血浆蛋白的吸附较低.所有结果显示该吸附剂具有很强的实际应用的潜力.%A series of bilirubin adsorbents with different ligand type, length and density were prepared by activation of Sepharose CL-4B and their adsorption capacities to bilirubin were measured in static adsorption experiments. The results showed that the structure and properties of ligands, such as sufficient spacer-length, higher grafting density on the adsorbent and the similar hydrophobicity with bilirubin, were helpful to improve adsorption capacity of bilirubin. These experiments provided some ideas for designing new adsorbents. Among these adsorbents, the adsorbent with 73μmol/mL butylamine and 23-atom spacer showed the strongest adsorption ability to bilirubin in BSA-bilirubin solution, which could reach the adsorption balance at 30°C in lh, and got binding capacity of 3.01mg/mL gel. Moreover, higher temperature was more favorable to the adsorption of bilirubin. The in vitro hemoperfusion experiments were carried out by adding 50mL adsorbent into 500mL patient's plasma and 55.2% of bilirubin (277.4mg/L) was removed after 2.5h, and at the same time, adsorption for plasma proteins was low. These results indicated that the Butylamine-Sepharose adsorbents could be a potential choice for specific

  3. Study on serum levels of lipids and bilirubin in patients with coronary heart disease%冠心病患者血脂和胆红素变化的研究

    Institute of Scientific and Technical Information of China (English)

    韦光海

    2008-01-01

    Objective To investigate the relationship of the levels of serum lipids and bilirubin with occurrence and development of coronary heart disease (CHD). Methods 139 CHD patients and 118 healthy controls were involved in the study. The serum levels of triglyeride (TG), total cholester-ol (TC), high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C)and bilirubin were measured and compared. Results In CHD group, the levels of TG, TC, LDL-C,TG, HDL-C, LDL-C and HDL-C were significantly elevated, while the levels of HDL-C, total biliru-bin (Tbil) and indirect bilirubin (Ibil) were markedly declined. The changes were associated with the severity of coronary artery disease. There wasn't significant difference of direct bilirubin level between CHD group and healthy control group. Conclusion Blood lipid abnormality and declination of bilirubin level are closely associated with coronary heart disease. It is possible to prevent the occurrence of car-diovascular event by early intervention.%目的 探讨血脂、胆红素与冠心病发生、发展的关系.方法 随机抽取139例冠心病患者和118例健康者进行血清甘油三酯(TG)、总胆固醇(TC)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)及胆红素水平检测与对比分析.结果 冠心病组患者TG、TC、LDL-C、TG/HDL-C、LDL-C/HDL-C显著升高(P0.05).结论 血脂异常和胆红素水平降低与冠心病密切相关.早期干预有可能预防心血管事件的发生.

  4. 经皮测胆红素在新生儿高胆红素血症中的应用价值%The Application Value of Transcutaneous Bilirubin in Neonatal Hyperbilirubinemia

    Institute of Scientific and Technical Information of China (English)

    周琼; 张飞忠

    2014-01-01

    Objectiv:To investigate the application value of transcutaneous bilirubin (TCB) in neonatal hyperbilirubinemia. Methods:215 cases of children with hyperbilirubinemia were dynamically tested by transcutaneous bilirubin method and blood bilirubin method at 6h, 12h, 2d, 4d, 7d and other time points respectively after the admission, and the correlation of the two sets of data was compared.Results:the values measured by transcutaneous bilirubin method and blood bilirubin method at 6h, 12h, 2d, 4d, 7d and other time points showed a linear correlation relationship (P<0.05).Conclusion:TCB has many advantages such as non-invasive, repeatable testing, easy to perform, rapid detection, dynamic monitoring of neonatal bilirubin changes, etc. it deserves clinical application.%目的::探讨经皮测胆红素(TCB)在新生儿高胆红素血症中的应用价值。方法:在入院第6h、12h、2d、4d、7d等时间点采用经皮测胆红素和经血测胆红素方法对215例高胆红素血症患儿进行动态检测,比较两组数据的相关性。结果:经皮测胆红素值和经血测胆红素值在新生儿入院第6h、12h、2d、4d、7d等时间点呈直线相关性关系(P<0.05)。结论:TCB具有无创伤性、可重复多次检测、操作简单易学、检测迅速、可动态监测新生儿胆红素变化情况等优点,值得临床推广使用。

  5. Exome-Wide Association Study Identifies New Low-Frequency and Rare UGT1A1 Coding Variants and UGT1A6 Coding Variants Influencing Serum Bilirubin in Elderly Subjects: A Strobe Compliant Article.

    Science.gov (United States)

    Oussalah, Abderrahim; Bosco, Paolo; Anello, Guido; Spada, Rosario; Guéant-Rodriguez, Rosa-Maria; Chery, Céline; Rouyer, Pierre; Josse, Thomas; Romano, Antonino; Elia, Maurizzio; Bronowicki, Jean-Pierre; Guéant, Jean-Louis

    2015-06-01

    Genome-wide association studies (GWASs) have identified loci contributing to total serum bilirubin level. However, no exome-wide approaches have been performed to address this question. Using exome-wide approach, we assessed the influence of protein-coding variants on unconjugated, conjugated, and total serum bilirubin levels in a well-characterized cohort of 773 ambulatory elderly subjects from Italy. Coding variants were replicated in 227 elderly subjects from the same area. We identified 4 missense rare (minor allele frequency, MAF bilirubin level (P = 2.34 × 10(-34), P = 7.02 × 10(-34), and P = 8.27 × 10(-34)), as well as unconjugated, and conjugated bilirubin levels. We also identified UGT1A6 variants in association with total (rs6759892, p.Ser7Ala, P = 1.98 × 10(-26); rs2070959, p.Thr181Ala, P = 2.87 × 10(-27); and rs1105879, p.Arg184Ser, P = 3.27 × 10(-29)), unconjugated, and conjugated bilirubin levels. All UGT1A1 intronic variants (rs887829, rs6742078, and rs4148325) and UGT1A6 coding variants (rs6759892, rs2070959, and rs1105879) were significantly associated with gallstone-related cholecystectomy risk. The UGT1A6 variant rs2070959 (p.Thr181Ala) was associated with the highest risk of gallstone-related cholecystectomy (OR, 4.58; 95% CI, 1.58-13.28; P = 3.21 × 10(-3)). Using an exome-wide approach we identified coding variants on UGT1A1 and UGT1A6 genes in association with serum bilirubin level and hyperbilirubinemia risk in elderly subjects. UGT1A1 intronic single-nucleotide polymorphisms (SNPs) (rs6742078, rs887829, rs4148324) serve as proxy markers for the low-frequency and rare UGT1A1 variants, thereby providing mechanistic explanation to the relationship between UGT1A1 intronic SNPs and the UGT1A1 enzyme activity. UGT1A1 and UGT1A6 variants might be potentially associated with gallstone-related cholecystectomy risk.

  6. 血清胆红素和血脂对冠心病发生的作用%Effection of serum bilirubin and serum lipid on coronary heart disease

    Institute of Scientific and Technical Information of China (English)

    李现立; 郑晓晖; 郝增光; 杨睿; 葛慧敏

    2010-01-01

    目的 探讨血清胆红素和血脂在冠心病发生中的作用.方法 75例冠心病患者被分为高脂血症组(46例)和非高脂血症组(29例),35例正常健康成人作为对照组,分别测定血清总胆红素(TBIL)、直接胆红素(DBIL)、间接胆红素(IBIL)以及总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白(LDL)和高密度脂蛋白(HDL)水平等指标作对比分析.结果 高脂血症组TBIL、DBIL、IBIL均低于非高脂血症组和正常对照组,差异有统计学意义(P<0.05);非高脂血症组血清胆红素水平低于正常对照组,差异有统计学意义(P<0.05).高脂血症组同非高脂血症组、正常对照组比较,TC、TG和LDL增高,HDL降低,差异有统计学意义(P<0.05);非高脂血症组同正常对照组比较,TC、TG和LDL增高,HDL降低,但差异无统计学意义(P>0.05).结论 低胆红素水平在高脂血症和非高脂血症的冠心病发生中起着重要的作用,是冠心病的独立危险因素.%Objective To investigate the effection of serum bilirubin and serum lipid on coronary heart disease (CHD). Methods Seventy -five patients with CHD group were divided into hyperlipidemia group(46 cases) and non- hyperlipidemia group (29 cases),35 healthy controls (healthy control group)were enrolled in the study. Serum total bilirubin (TBIL), direct bilirubin (DBIL), indirect bilirubin (IBIL)serum triglycride ( TG), total cholestrol ( TC ), low density lipoprotein (LDL), high density lipoprotein (HDLand serum lipid were examined for all subiects,and then the results were compared and analyzed. Results The contents of serum TBIL, direct bilirubin (DBIL), indirect bilirubin (IBIL)were lower in hyperlipidemia group than those of non - hyperlipidemia and healthy control group, the difference was significant (P <0. 05 ). The contents of serum bilirubin were lower in non - hyperlipidemia group than those of healthy control group, the difference was significant( P < 0.05 ). TC,TG and LDL were

  7. Validation of transcutaneous bilirubin nomogram for identifying neonatal hyperbilirubinemia in healthy Chinese term and late-preterm infants: a multicenter study

    Directory of Open Access Journals (Sweden)

    Zhangbin Yu

    2014-06-01

    Full Text Available OBJECTIVE: to prospectively validate a previously constructed transcutaneous bilirubin (TcB nomogram for identifying severe hyperbilirubinemia in healthy Chinese term and late-preterm infants. METHODS: this was a multicenter study that included 9,174 healthy term and late-preterm infants in eight hospitals of China. TcB measurements were performed using a JM-103 bilirubinometer. TcB values were plotted on a previously developed TcB nomogram, to identify the predictive ability for subsequent significant hyperbilirubinemia. RESULTS: in the present study, 972 neonates (10.6% developed significant hyperbilirubinemia. The 40th percentile of the nomogram could identify all neonates who were at risk of significant hyperbilirubinemia, but with a low positive predictive value (PPV (18.9%. Of the 453 neonates above the 95th percentile, 275 subsequently developed significant hyperbilirubinemia, with a high PPV (60.7%, but with low sensitivity (28.3%. The 75th percentile was highly specific (81.9% and moderately sensitive (79.8%. The area under the curve (AUC for the TcB nomogram was 0.875. CONCLUSIONS: this study validated the previously developed TcB nomogram, which could be used to predict subsequent significant hyperbilirubinemia in healthy Chinese term and late-preterm infants. However, combining TcB nomogram and clinical risk factors could improve the predictive accuracy for severe hyperbilirubinemia, which was not assessed in the study. Further studies are necessary to confirm this combination.

  8. Enhancement of the performances of a single concentric glucose/O{sub 2} biofuel cell by combination of bilirubin oxidase/Nafion cathode and Au-Pt anode

    Energy Technology Data Exchange (ETDEWEB)

    Habrioux, A.; Servat, K.; Kokoh, K.B. [LACCO ' ' Equipe Electrocatalyse' ' , UMR 6503 CNRS-Universite de Poitiers, 40 Avenue du Recteur Pineau, 86022 Poitiers (France); Tingry, S. [Institut Europeen des Membranes, UMR 5635, ENSCM-UMII-CNRS Place Eugene Bataillon, CC 047, 34095 Montpellier, Cedex 5 (France)

    2009-01-15

    This work deals with a novel preparation method of bilirubin oxidase/2,2'-azinobis-3-ethylbenzothiazoline-6-sulfonic acid electrode. The enzyme and its mediator were adsorbed on carbon Vulcan XC-72R before their immobilization into a Nafion {sup registered} matrix. Promising results were obtained when this biocathode was associated with Au{sub 70}Pt{sub 30} nanoparticles as anode in a single concentric glucose/O{sub 2} biofuel cell (BFC). The latter BFC delivered at 37 C a power density of 90 {mu}W cm{sup -2} for a cell voltage of 0.4 V in phosphate buffer (pH 7.4) containing 0.01 M glucose. Moreover, the electrical performances were increased with the concentration of glucose by generating up to 190 {mu}W cm{sup -2} for a cell voltage of 0.52 V when the concentration of the renewable fuel reached 0.7 M. (author)

  9. On the impact of oxytocin on high neonatal bilirubin levels, clinical studies%催产素对新生儿高胆红素血症影响的临床研究

    Institute of Scientific and Technical Information of China (English)

    罗嫚丽

    2016-01-01

    Objective to explore the application of oxytocin during labor and maternal high different dose of oxytocin on neonatal bilirubin hematic disease, provide guidance for clinical. Methods to select 339 full-term healthy newborns born to our hospital patients as the research object, according to whether or not to use in the process of childbirth oxytocin is divided into two groups, 223 cases of patients who did not use the oxytocin neonatal as control group, and use of oxytocin, 116 cases of neonatal as observation group, and all 3, 5, and 7 days after birth detect neonatal bilirubin, was born in 7 d after record high neonatal bilirubin levels and the incidence of asphyxia. Results in the control group after the birth of blood bilirubin value ex-ists significant difference, and after the birth of 3 d, 5 d after birth no difference, after the birth of the 7 d significantly reduced, the observation group after the birth of blood bilirubin value exists significant difference, and after the birth of 3 d, 5 d after birth there is significant difference, after the birth of the 7 d significantly reduced, between the two groups in 3 d, 5 d, 7 d after birth blood bilirubin value exists significant difference; In 3 d after birth, the different dose of oxytocin blood bilirubin value differ-ences between groups, 5 IU group and 10 IU group is significantly higher than 1.5 IU group and 2.5 IU group, 5 d and 7 d after birth, blood bilirubin values between different dose of oxytocin group differences, with the increase of dosage, blood bilirubin values significantly increased; Newborn on the incidence of high blood bilirubin, control group was 21.97% (49/223), the ob-servation group of newborns was 37.93% (44/116), there were significant differences; Newborn on the incidence of asphyxia, control group was 4.04% (9/223), the observation group of newborns was 14.66% (17/116), there were significant differences.Conclusion pregnant women in childbirth using oxytocin but high neonatal

  10. Fabrication of high performance bioanode based on fruitful association of dendrimer and carbon nanotube used for design O2/glucose membrane-less biofuel cell with improved bilirubine oxidase biocathode.

    Science.gov (United States)

    Korani, Aazam; Salimi, Abdollah

    2013-12-15

    In this study, the preparation of an integrated modified electrode based on the covalent attachment of glucose dehydrogenase (GDH) enzyme and safranin O to amine-derivative multiwalled carbon nanotubes (MWCNTs-NH2) modified glassy carbon (GC) electrode using G2.5-carboxylated PAMAM dendrimer (Den) as linking agent is reported. The obtained results indicated that the proposed system has effective bioelectrocatalytic activity toward glucose oxidation at 100 mV with onset potential of -130 mV (vs. Ag/AgCl). The performance of the prepared hybrid system of GC/MWCNTs-NH2/Den/GDH/Safranin as anode in a membraneless enzyme-based glucose/O2 biofuel cell is further evaluated. The biocathode in this system was composed of bilirubin oxidase (BOX) enzyme immobilized onto a bilirubin modified carbon nanotube GC electrode. Immobilized BOX onto CNTs/bilirubin not only show direct electron transfer but also it has excellent electrocatalytic activity toward oxygen reduction at a positive potential of 610 mV. The open circuit voltage of the cell was 590 mV. The maximum current density was 0.5 mA cm(-2), while maximum power density of 108 μW cm(-2) was achieved at voltage of 330 mV. The immobilized enzymes in anode and cathode are very stable and output power of the BFC is approximately constant after 12 h continues operation.

  11. 吸附于银胶颗粒表面的胆红素及胆绿素分子吸附取向研究%The Study of Orientations of Bilirubin and Biliverdin by Surface-enhanced Raman Spectroscopy

    Institute of Scientific and Technical Information of China (English)

    胡军; 方清; 盛蓉生; 徐知三; 曾云鹗

    2001-01-01

    胆红素、胆绿素作为重要的生物小分子被广泛研究。本文采用表面增强拉曼光谱技术获得了胆红素和胆绿素分子吸附于银胶颗粒表面的拉曼光谱。通过对其谱带的指认分析,表明胆红素形成了三对内氢键,并以其两个吡咯亚甲基酮环沿银胶颗粒表面切向方向镶嵌在银胶颗粒上,而胆绿素则以顺式-顺式-顺式构型平躺吸附于银胶颗粒表面。%The surface-enhanced Raman spectra of bilirubin and biliverdin were obtained.By the bands analysis of the spectra,the orientation of bilirubin and biliverdin on the surface of silver colloid was discussed.In such case,the bilirubin was adsorbed on the silver colloid particle with the two planar pyrromethenone groups intercalated into the globe silver colloid particle,however,the biliverdin might lie flat on the surface of silver colloid with syn-synsyn conformation.

  12. Comparação entre a dosagem transcutânea e plasmática de bilirrubina Comparison of transcutaneous and plasma bilirubin measurement

    Directory of Open Access Journals (Sweden)

    Maria das Graças da Cunha Leite

    2007-06-01

    Full Text Available OBJETIVOS: Comparar dosagens transcutâneas de bilirrubina pelo Bilicheck com a dosagem plasmática capilar pelo bilirrubinômetro Unistat (Leica. MÉTODOS: Foram realizadas 200 dosagens concomitantes (transcutânea e plasmática, calculadas a correlação e concordância entre elas e feita avaliação da influência do peso de nascimento, raça, idade gestacional, idade pós-natal e uso de fototerapia. RESULTADOS: A correlação linear foi de 0,92, e a média da diferença entre as dosagens foi de 0,72 (±1,57 mg/dL, com intervalo de confiança em 95% de -2,42 a +3,86. A curva ROC realizada com a dosagem transcutânea em 14 mg/dL demonstrou melhor sensibilidade (88,2% e especificidade (97,8%, com valor preditivo positivo de 78,9%, valor preditivo negativo de 98,9 e área abaixo da curva de 0,98. CONCLUSÃO: A dosagem realizada pelo Bilicheck pode substituir a dosagem plasmática capilar até o valor de 14 mg/dL. Acima deste nível, deve ser considerada apenas como rastreador na seleção de pacientes que devem ser submetidos a dosagem sangüínea.OBJECTIVES: To compare transcutaneous bilirubin measurements made using Bilicheck equipment with assays of capillary plasma using the Unistat bilirubinometer (Leica. METHODS: Two hundred concomitant assays were performed (transcutaneous and in plasma, and the correlation and level of agreement between them was calculated. An assessment was also made of the influence of birth weight, skin color, gestational age, postnatal age and phototherapy. RESULTS: The linear correlation coefficient was 0.92, and the mean difference between assays was 0.72 (±1.57 mg/dL, with a 95% confidence interval from -2.42 to +3.86. The best of a series of ROC curves demonstrated that transcutaneous assays at 14 mg/dL offer the best sensitivity (88.2% and specificity (97.8%, with a positive predictive value of 78.9%, negative predictive value of 98.9 and are below the curve of 0.98. CONCLUSIONS: Assays performed using

  13. Combining serum cystatin C with total bilirubin improves short-term mortality prediction in patients with HBV-related acute-on-chronic liver failure.

    Directory of Open Access Journals (Sweden)

    Zhihong Wan

    Full Text Available BACKGROUND & AIMS: HBV-related acute-on-chronic liver failure (HBV-ACLF is a severe liver disease which results in a high mortality in China. To early predict the prognosis of the patients may prevent the complications and improve the survival. This study was aimed to develop a new prognostic index to estimate the survival related to HBV-ACLF. METHODS: Consecutive patients with HBV-ACLF were included in a prospective observational study. Serum Cystatin C concentrations were measured by using the particle-enhanced immunonephelometry assay. All of the patients were followed for at least 3 months. Cox regression analysis was carried out to identify which factors were predictive of mortality. The area under the receiver operating characteristic curve (AUC was used to evaluate the efficacy of the variates for early predicting mortality. RESULTS: Seventy-two patients with HBV-ACLF were recruited between January 2012 and January 2013. Thirty patients died (41.7% during 3-months followed up. Cox multivariate regression analysis identified serum cystatin C (CysC and total bilirubin (TBil were independent factors significantly (P < 0.01 associated with survival. Our results further showed that new prognostic index (PI combining serum CysC with TBil was a good indicator for predicting the mortality of patients with HBV-ACLF. Specifically, the PI had a higher accuracy than the CTP, MELD, or MELD-Na scoring for early prediction short-term survival of HBV-ACLF patients with normal levels of serum creatinine (Cr. The survival rate in low risk group (PI < 3.91 was 94.3%, which was markedly higher than those in the high-risk group (PI ≥ 3.91 (17.4%, P < 0.001. CONCLUSION: We developed a new prognostic index combining serum CysC with TBil which early predicted the short-term mortality of HBV-ACLF patients.

  14. Membraneless enzymatic ethanol/O2 fuel cell: Transitioning from an air-breathing Pt-based cathode to a bilirubin oxidase-based biocathode

    Science.gov (United States)

    Aquino Neto, Sidney; Milton, Ross D.; Hickey, David P.; De Andrade, Adalgisa R.; Minteer, Shelley D.

    2016-08-01

    The bioelectrooxidation of ethanol was investigated in a fully enzymatic membraneless ethanol/O2 biofuel cell assembly using hybrid bioanodes containing multi-walled carbon nanotube (MWCNT)-decorated gold metallic nanoparticles with either a pyrroloquinoline quinone (PQQ)-dependent alcohol dehydrogenase (ADH) enzyme or a nicotinamide adenine dinucleotide (NAD+)-dependent ADH enzyme. The biofuel cell anode was prepared with the PQQ-dependent enzyme and designed using either a direct electron transfer (DET) architecture or via a mediated electron transfer (MET) configuration through a redox polymer, 1,1‧-dimethylferrocene-modified linear polyethyleneimine (FcMe2-C3-LPEI). In the case of the bioanode containing the NAD+-dependent enzyme, only the mediated electron transfer mechanism was employed using an electropolymerized methylene green film to regenerate the NAD+ cofactor. Regardless of the enzyme being employed at the anode, a bilirubin oxidase-based biocathode prepared within a DET architecture afforded efficient electrocatalytic oxygen reduction in an ethanol/O2 biofuel cell. The power curves showed that DET-based bioanodes via the PQQ-dependent ADH still lack high current densities, whereas the MET architecture furnished maximum power density values as high as 226 ± 21 μW cm-2. Considering the complete membraneless enzymatic biofuel cell with the NAD+-dependent ADH-based bioanode, power densities as high as 111 ± 14 μW cm-2 were obtained. This shows the advantage of PQQ-dependent ADH for membraneless ethanol/O2 biofuel cell applications.

  15. Review: Bilirubin pKa studies; new models and theories indicate high pKa values in water, dimethylformamide and DMSO

    Directory of Open Access Journals (Sweden)

    Ostrow J

    2010-03-01

    Full Text Available Abstract Background Correct aqueous pKa values of unconjugated bilirubin (UCB, a poorly-soluble, unstable substance, are essential for understanding its functions. Our prior solvent partition studies, of unlabeled and [14C] UCB, indicated pKa values above 8.0. These high values were attributed to effects of internal H-bonding in UCB. Many earlier and subsequent studies have reported lower pKa values, some even below 5.0, which are often used to describe the behavior of UCB. We here review 18 published studies that assessed aqueous pKa values of UCB, critically evaluating their methodologies in relation to essential preconditions for valid pKa measurements (short-duration experiments with purified UCB below saturation and accounting for self-association of UCB. Results These re-assessments identified major deficiencies that invalidate the results of all but our partition studies. New theoretical modeling of UCB titrations shows remarkable, unexpected effects of self-association, yielding falsely low pKa estimates, and provides some rationalization of the titration anomalies. The titration behavior reported for a soluble thioether conjugate of UCB at high aqueous concentrations is shown to be highly anomalous. Theoretical re-interpretations of data in DMSO and dimethylformamide show that those indirectly-derived aqueous pKa values are unacceptable, and indicate new, high average pKa values for UCB in non-aqueous media (>11 in DMSO and, probably, >10 in dimethylformamide. Conclusions No reliable aqueous pKa values of UCB are available for comparison with our partition-derived results. A companion paper shows that only the high pKa values can explain the pH-dependence of UCB binding to phospholipids, cyclodextrins, and alkyl-glycoside and bile salt micelles.

  16. Metastable and equilibrium phase diagrams of unconjugated bilirubin IXα as functions of pH in model bile systems: Implications for pigment gallstone formation.

    Science.gov (United States)

    Berman, Marvin D; Carey, Martin C

    2015-01-01

    Metastable and equilibrium phase diagrams for unconjugated bilirubin IXα (UCB) in bile are yet to be determined for understanding the physical chemistry of pigment gallstone formation. Also, UCB is a molecule of considerable biomedical importance because it is a potent antioxidant and an inhibitor of atherogenesis. We employed principally a titrimetric approach to obtain metastable and equilibrium UCB solubilities in model bile systems composed of taurine-conjugated bile salts, egg yolk lecithin (mixed long-chain phosphatidylcholines), and cholesterol as functions of total lipid concentration, biliary pH values, and CaCl2 plus NaCl concentrations. Metastable and equilibrium precipitation pH values were obtained, and average pKa values of the two carboxyl groups of UCB were calculated. Added lecithin and increased temperature decreased UCB solubility markedly, whereas increases in bile salt concentrations and molar levels of urea augmented solubility. A wide range of NaCl and cholesterol concentrations resulted in no specific effects, whereas added CaCl2 produced large decreases in UCB solubilities at alkaline pH values only. UV-visible absorption spectra were consistent with both hydrophobic and hydrophilic interactions between UCB and bile salts that were strongly influenced by pH. Reliable literature values for UCB compositions of native gallbladder biles revealed that biles from hemolytic mice and humans with black pigment gallstones are markedly supersaturated with UCB and exhibit more acidic pH values, whereas biles from nonstone control animals and patients with cholesterol gallstone are unsaturated with UCB.

  17. Application value of umbilical cord blood bilirubin levels in predicting neonatal jaundice%脐血胆红素水平在预测新生儿黄疸中的应用价值

    Institute of Scientific and Technical Information of China (English)

    李莎

    2015-01-01

    目的:探讨脐血胆红素水平在预测新生儿黄疸中的应用价值。方法选取2013年1月-2015年1月医院足月新生儿132例。于出生时、出生后24、48、72h 测定脐血胆红素水平,并按浓度分为﹤30μmol/L、≥30μmol/L、≥36μmol/L、≥42μmol/L 4组。所有新生儿均于出生时、生后24、48、72h测定经皮胆红素(测定新生儿前额及前胸2个部位,读数取平均值后记录),比较高胆红素症与非高胆红素血症组的脐血胆红素水平及不同脐血胆红素水平组高胆红素症发生率。结果新生儿脐血胆红素﹤30μmol/L组黄疸发生率为16.7%明显低于≥30μmol/L组的34.2%、≥36μmol/L组的73.3%及≥45μmol/L组的85.0%,比较差异均有统计学意义( P﹤0.05)。非高胆红素组脐血胆红素、出生后24、48、72h经皮胆红素指数均低于高胆红素组,比较差异均有统计学意义( P﹤0.05)。结论高胆红素血症新生儿的脐血胆红素相应升高,测定脐血胆红素对预测新生儿黄疸有实际的应用价值。%Objective To eXplore the application Value of umbilical cord blood bilirubin leVels in predicting neonatal jaundice. Methods Select 132 cases of term newborns. The leVel of cord blood bilirubin was measured at birth,and at 24 hours,48 hours and 72 hours after birth,according to the concentration diVided into( ﹤30μmol/L)group,(≥30μmol/L) group,(≥36μmol/L)group and(≥42μmol/L)group. The leVel of transcutaneous bilirubinometer was also measured( De-termination of the forehead and chest in two parts,taking the aVerage Value)at birth,and at 24 hours,48 hours and 72 hours after birth. comparison high bilirubin group and non hyperbilirubinemia group cord blood bilirubin leVel,and the incidence of high bilirubin in different leVels of serum bilirubin. Results Neonatal umbilical cord blood bilirubin﹤30μmol/L jaundice in-cidence was 16. 7%μmol/L group was obViously lower acuity 30

  18. 血清胆红素与尿酸检验在冠心病患者诊断中的价值分析%Value of Serum Bilirubin and Uric Acid Test in the Diagnosis of Patients With Coronary Heart Disease

    Institute of Scientific and Technical Information of China (English)

    代劲

    2015-01-01

    Objective Analysis of serum bilirubin and uric acid value to the diagnosis of coronary heart disease.MethodsSelect 80 cases of coronary heart disease patients as observation group,in the same period 80 cases of healthy subjects as control group,serum bilirubin and uric acid in test,compare test results.Results Observation group serum bilirubin level is lower than the control group,uric acid level is higher than that of control group(P<0.05); Observation group of total bilirubin,combination of bilirubin,the bilirubin decreased as the Gensini score,uric acid wil increase (P<0.05). Conclusion Inspection of serum bilirubin and uric acid levels to provide a strong basis for the diagnosis and treatment of coronary heart disease.%目的:分析血清胆红素与尿酸对冠心病的诊断价值。方法选取80例冠心病患者为观察组,同期80例健康者为对照组,均行血清胆红素和尿酸检验,比较检测结果。结果观察组血清胆红素水平低于对照组,尿酸水平则高于对照组(P<0.05);观察组总胆红素、结合胆红素、非结合胆红素随着Gensini积分的增加而减少,尿酸则会增加(P<0.05)。结论检验血清胆红素和尿酸水平可为诊疗冠心病提供有力依据。

  19. Self-assembly of aqueous bilirubin ditaurate, a natural conjugated bile pigment, to contraposing enantiomeric dimers and M(-) and P(+) tetramers and their selective hydrophilic disaggregation by monomers and micelles of bile salts.

    Science.gov (United States)

    Neubrand, Michael W; Carey, Martin C; Laue, Thomas M

    2015-02-24

    The solution behavior of bilirubin ditaurate (BDT), the first naturally occurring conjugated bile pigment to be physically and chemically characterized, was assessed in aqueous solution and in monomeric and micellar solutions of common taurine-conjugated bile salts (BS). Analytical ultracentrifugation revealed that BDT self-associates in monomer-dimer equilibria between 1 and 500 μM, forming limiting tetramers at low millimolar concentrations. Self-association was enthalpically driven with ΔG values of ≈5 kcal/mol, suggesting strong hydrophobic interactions. Added NaCl and decreases in temperature shifted the oligomerization to lower BDT concentrations. On the basis of circular dichroism spectra and the limiting size of the self-aggregates, we infer that the tetramers are composed of 2P(+) and 2M(-) enantiomeric BDT pairs in "ridge-tile" conformations interacting in a "double-bookend" structure. With added monomeric BS, blue shifts in the UV-vis spectra and tight isosbestic points revealed that BDT/BS heterodimers form, followed by BDT "decorating" BS micelles mostly via hydrophilic interactions. Conformational enantiomerism, fluorescence intensities, and anisotropy, as well as resistance of the hybrid particles to disaggregation in 6 M urea, suggested that two or three hydrogen-bonding sites bound BDT monomers to the hydroxyl groups of BS, possibly via pyrrole-π-orbital-OH interactions. BDT stabilized these interactions by enveloping the BS in its "ridge-tile" pincers with variable strain that maximized van der Waals interactions. Possibly because the BDT molecule becomes highly strained with BS subtending a 7β-hydroxyl group, BDT became totally resistant to oxidation in air. This work predicts that, because of BS dissolution of the BDT self-aggregates, BS/bilirubin hybrid particles, which are stabilized hydrophilically, are likely to be the dominant mode of transport for all conjugated bilirubins in bile.

  20. Study on extraction of chenodeoxycholic acid and bilirubin fromgoose gallbladder%鹅胆汁中鹅脱氧胆酸和胆红素提取工艺研究

    Institute of Scientific and Technical Information of China (English)

    贾霖; 黄国清; 王宝维; 肖军霞

    2012-01-01

    Both chenodeoxycholic acid and bilirubin are the main active components of goose gallbladder.The chenodeoxycholic acid and bilirubin were isolated and extracted from the goose gallbladder in the same time through the steps of saponification,neutralization,delamination and so on.The effect of saponification temperature,time and sodium hydroxide concentration were determined through the single factor and orthogonal tests.The results showed that the optimum conditions were as follows:saponification temperature 90℃,1h,sodium hydroxide concentration 8%.Under these conditions,the yield of chenodeoxycholic acid reached 0.9mg/g,the yield of bilirubin reached 0.46mg/g.%鹅脱氧胆酸(CDCA)和胆红素均是鹅胆中的主要活性成分。将鹅胆通过皂化、中和、分层等步骤,同时从中分离提取出鹅脱氧胆酸和胆红素。通过单因素实验和正交实验确定了皂化温度、皂化时间和NaOH浓度的最佳条件。实验结果表明,当皂化温度为90℃,皂化时间为1h,NaOH浓度为8%时,CDCA的提取量为0.9mg/g,胆红素的提取量为0.46mg/g。

  1. Relationship between Serum Bilirubin and LDL - C Levels in Patients with Coronary Heart Disease%冠心病患者血清胆红素与低密度脂蛋白胆固醇水平的关系研究

    Institute of Scientific and Technical Information of China (English)

    张慧; 张玉顺

    2015-01-01

    目的:探讨冠心病患者血清胆红素与低密度脂蛋白胆固醇( LDL-C)水平的关系。方法选择2013年9月—2014年9月西安交通大学医学院第一附属医院收治的冠心病患者55例作为观察组,另选择同期在西安交通大学医学院第一附属医院进行体检的健康者60例作为对照组。检测两组受检者血清总胆红素、直接胆红素、间接胆红素及LDL-C水平,并分析冠心病患者血清胆红素与LDL-C水平的关系。结果观察组患者血清总胆红素、直接胆红素及间接胆红素水平低于对照组,LDL-C水平高于对照组( P<0.05)。观察组患者血清总胆红素、直接胆红素、间接胆红素水平与LDL-C水平均呈负相关(r值分别为-0.72、-0.31、-0.41,P<0.05)。结论冠心病患者血清胆红素与LDL-C水平均出现异常改变,且血清LDL-C水平随胆红素水平上升而下降。%Objective To explore the relationship between serum bilirubin and LDL - C levels in patients with coronary heart disease. Methods From September 2013 to September 2014,55 patients with coronary heart disease were selected as observation groups in the First Affiliated Hospital of Xi′an Jiaotong University Medicine School,and 60 healthy cases were selected as control group at the same time. Serum levels total bilirubin,direct bilirubin,indirect bilirubin and LDL-C of the two groups were detected,and the relationship between serum bilirubin and LDL-C levels was analyzed. Results Serum levels total bilirubin,direct bilirubin,indirect bilirubin of observation group were lower than those of control group,that of LDL-C was higher than that of control group ( P <0. 05 ). Serum levels total bilirubin, direct bilirubin, indirect bilirubin of observation group was negatively correlated with serum LDL - C level,respectively( r = -0. 72,-0. 31,-0. 41,P <0. 05). Conclusion The serum bilirubin and LDL-C levels of patients with coronary heart disease are abnormal

  2. Endothelial cells derived from the blood-brain barrier and islets of Langerhans differ in their response to the effects of bilirubin on oxidative stress under hyperglycemic conditions

    Directory of Open Access Journals (Sweden)

    Jaime eKapitulnik

    2012-07-01

    Full Text Available Unconjugated bilirubin (UCB is a neurotoxic degradation product of heme. Its toxic effects include induction of apoptosis, and ultimately neuronal cell death. However, at low concentrations, UCB is a potent antioxidant that may protect cells and tissues against oxidative stress by neutralizing toxic metabolites such as reactive oxygen species (ROS. High glucose levels (hyperglycemia generate reactive metabolites. Endothelial cell dysfunction, an early vascular complication in diabetes, has been associated with hyperglycemia-induced oxidative stress. Both glucose and UCB are substrates for transport proteins in microvascular endothelial cells of the blood-brain barrier (BBB. In the current study we show that UCB (1-40 M induces apoptosis and reduces survival of bEnd3 cells, a mouse brain endothelial cell line which serves as an in vitro model of the BBB. These deleterious effects of UCB were enhanced in the presence of high glucose (25 mM levels. Interestingly, the bEnd3 cells exhibited an increased sensitivity to the apoptotic effects of UCB when compared to the MS1 microcapillary endothelial cell line. MS1 cells originate from murine pancreatic islets of Langherans, and are devoid of the barrier characteristics of BBB-derived endothelial cells. ROS production was increased in both bEnd3 and MS1 cells exposed to high glucose, as compared with cells exposed to normal (5.5 mM glucose levels. While UCB (0.1-40 M did not alter ROS production in cells exposed to normal glucose, relatively low ('physiological' UCB concentrations (0.1-5 M attenuated ROS generation in both cell lines exposed to high glucose levels. Most strikingly, higher UCB concentrations (20-40 M increased ROS generation in bEnd3 cells exposed to high glucose, but not in similarly treated MS1 cells. These results may be of critical importance for understanding the vulnerability of the BBB endothelium upon exposure to increasing UCB levels under hyperglycemic conditions.

  3. Analysis the Risk Factor of Bilirubin Encephalopathy of Neonatal Hyperbilirubinemia%新生儿高胆红素血症胆红素脑病的危险性因素分析

    Institute of Scientific and Technical Information of China (English)

    陈思远; 覃睿; 黄国坚; 叶军; 陈媚; 周江; 周转智

    2015-01-01

    Objective Analysis the risk factor of bilirubin encephalopathy of Neonatal .And provides the measurement to decrease hy-perbilirubinemia bilirubin encephalopathy incidence.Methods Retrospective analysis 209 neonatal hyperbilirubinemia patients′clinical data.Definite bind the score 7-9 points as acute bilirubin encephalopathy (ABE).the children ABE as the observation group (n=40 pa-tients), the rest patients as the control group (n=169 patients).Related factors that may affect the incidence of hyperbilirubinemia ABE were univariate and multivariate Logistic regression analysis, the Related factors include gender, neonatal gestational age, birth weight, mode of delivery, fetal membranes early break, feeding patterns, gestational diabetes, gestational hypertension, asphyxia or fetal distress,the peak total bilirubin, jaundice family history, RH hemolytic disease and ABO hemolytic disease.Results It was found that birth weight (OR=3.739,95%CI:1.240 -11.880), total bilirubin peak (OR=6.475,95%CI:1.724 -17.419), RH hemolytic disease(OR=11.473,95%CI:2.708-33.697)and ABO hemolytic disease (OR=9.143,95%CI:2.492-28.513)were the risk factors for neonatal hy-perbilirubinemia bilirubin encephalopathy after multivariate Logistic regression analysis, P<0.05.Conclusion Birth weight, total biliru-bin peak, RH hemolytic disease and ABO hemolytic disease are related to bilirubin encephalopathy, clinicians should strengthen Screening and treat the very low birth weight, high total bilirubin peak, accompanied by RH hemolytic disease and ABO hemolytic patients.%目的:分析新生儿高胆红素血症胆红素脑病的危险性因素,为预防高胆红素血症胆红素脑病提供思路。方法回顾性分析我院收治的209例新生儿高胆红素血症患儿临床资料,将胆红素脑病致使神经功能系统障碍( bilirubin-induced neurologic dysfunction,Bind)评分为7~9分的患儿定义为急性胆红素脑病(ABE)患儿

  4. Effect of bilirubin on the function of T lymphocytes of premature infants and the mechanism%胆红素对早产儿T淋巴细胞功能的影响及相关机制的研究

    Institute of Scientific and Technical Information of China (English)

    马琳; 江莲

    2012-01-01

    目的:探讨不同血清胆红素水平对早产儿T淋巴细胞功能的影响.方法:应用流式细胞仪法测定不同胎龄、不同胆红素水平下CD4+、CD8+、白细胞介素-4(Interleukin-4、IL-4)、肿瘤坏死因子-γ(Tumor necrosis factor-γ、IFN-v)、Fas基因表达.结果:①胎龄≥32周早产儿的CD4+和CD8+表达量>胎龄<32周早产儿,差异有统计学意义(P<0.05).②不同胆红素浓度对早产儿T淋巴细胞功能的影响:胆红素153.9~220.6 μmol/L组和胆红素>220,6 μmol/L组早产儿CD4+的表达水平均明显低于无黄疸组和胆红素<153.9 μmol/L组(P<0.05);胆红素>220.6 μmol/L组早产儿CD8+的表达水平低于无黄疸组、胆红素<153.9 μmol/L组和胆红素153.9-220.6 μmol/L组(P<0.05).IL-4的表达量与4组比较无差异(P>0.05).IFN-γ的表达量于胆红素153.9~220.6 μmol/L组和胆红素>220.6 μmol/L组均<无黄疸组和胆红素<153.9 μmol/L组(P<0.05).Fas基因的表达量于胆红素153.9~220.6 μmol/L组和胆红素>220.6 μmol/L组均高于无黄疸组和胆红素< 153.9 μmoL/L组(P<0.05).结论:①胎龄是影响早产儿T淋巴细胞功能的重要因素之一,胎龄越小免疫功能越低.②黄疸对早产儿T淋巴细胞功能有显著抑制作用,随胆红素浓度的增加抑制作用越显著,同时Fas基因的表达量逐渐增高,提示此种抑制作用与淋巴细胞凋亡增多有关.%Objective: To explore the effects of different levels of serum bilirubin on the function of T lymphocytes of premature infants. Methods: Flow cytometry was used to detect the expression levels of CD4+ , CD8+, interleukin -4, tumor necrosis factor-γ, and Fas gene among the premature infants of different fetal ages and bilirubin levels. Results: The expression levels of CD4 + and CD8 + in premature infants of fetal age ≥32 weeks were significantly higher than those in premature infants of fetal age 220.6 μmol/L group were significantly lower than

  5. 胆红素/清蛋白比值及脑干听觉诱发电位与胆红素神经毒性的相关性%Relationship between bilirubin/albumin ratio, auditory brainstem responses and bilirubin-induced neurotoxicity

    Institute of Scientific and Technical Information of China (English)

    王丹; 杨波; 郑瑞平

    2013-01-01

    目的 探讨高胆红素血症患儿血清总胆红素(TBIL)、总胆红素/清蛋白比值(B/A比值)与胆红素神经毒性的相关性,分析高胆红素血症患儿脑干听觉诱发电位(ABRs)中脑干功能损伤及听神经功能损伤敏感程度.方法 将91例足月高胆红素血症患儿根据ABRs结果分为A组(未见明显异常)、B组(仅脑干功能受损)、C组(仅听神经受损)、D组(脑干功能及听神经均受损),比较各组间TBIL、B/A比值的差异并分析二者与ABRs的关系.结果 A组与B组比较,TBIL差异无统计学意义(t=1.566,P>0.05),B/A比值差异有统计学意义(t=1.898,P<0.05);B组与C组TBIL、B/A比值比较差异均有统计学意义(tTBIL=2.372,PTBIL<0.05;tB/A=2.794,PB/A<0.01);A组与C组比较、A组与D组比较、B组与D组比较,TBIL比值差异均有统计学意义(t =2.452、3.328、2.256,P均<0.05);A组与C组比较、A组与D组比较、B组与D组比较,B/A比值差异均有统计学意义(=2.571、4.680、2.657,P均<0.05、0.01);C组与D组比较,TBIL、B/A比值差异均无统计学意义(tTBIL=0.705,PTBIL >0.05;tB/A=0.636,PB/A >0.05).TBIL、B/A比值的受试者工作特征曲线(ROC)下面积分别为0.919、0.829.TBIL、B/A比值与ABRs有显著相关性(r=0.495、0.564,P均<0.05).结论 B/A比值是预测足月儿胆红素神经毒性的良好指标,ABRs异常中脑干功能较听神经易受损.%Objective To explore the relationship between serum total bilirubin (TBIL),total bilirubin/albumin ratio (B/A ratio) and bilirubin-induced neurotoxicity,and to analyze the sensitivity of brainstem function and auditory nerval function in auditory brainstem responses (ABRs) in full-term newborn infants with hyperbilirubinemia.Methods Ninety-one cases of full-term neonates with hyperbilirubinemia were divided into 4 groups according to the findings of ABRs:group A(with normal ABRs),group B (only brainstem dysfunction),group C (only auditory neural damage),group D

  6. Relationship between serum bilirubin, fibrinogen and diabetic nephropathy in patients with type 2 diabetes mellitus%2型糖尿病患者血清胆红素、纤维蛋白原与糖尿病肾病的相关性研究

    Institute of Scientific and Technical Information of China (English)

    张玉海; 刘海蔚; 陈道雄; 高勇义

    2015-01-01

    目的:探讨2型糖尿病(T2DM)患者血清胆红素及纤维蛋白原与糖尿病肾病(DN)发生的关系。方法629例T2DM患者,按照24 h尿白蛋白定量分为正常尿白蛋白组(A组)474例,微量尿白蛋白组(B组)100例,临床尿白蛋白组(C组)55例。并以146例正常成人作为对照组,观察各组之间血清胆红素和纤维蛋白原的差异,探讨胆红素及纤维蛋白原与DN相关性。结果 T2DM患者血清总胆红素、直接胆红素和间接胆红素水平均低于正常对照组(P<0.05),纤维蛋白原高于对照组(P<0.05)。C组总胆红素、间接胆红素、直接胆红素均较A组及B组降低(P<0.05),C组纤维蛋白原较正常A组及B组升高(P<0.05),B组总胆红素、间接胆红素、直接胆红素均较A组下降(P<0.05),B组纤维蛋白原较A组升高(P<0.05),将尿白蛋白与血清胆红素水平及纤维蛋白原作相关性分析,结果显示尿白蛋白与总胆红素、直接胆红素、间接胆红素呈负相关,相关系数分别为r=-0.191,P<0.05;r=-0.299,P<0.05;r=-0.184,P<0.05。尿白蛋白与纤维蛋白原呈正相关,相关系数为r=0.33,P<0.05。总胆红素、直接胆红素、间接胆红素与纤维蛋白原呈负相关,相关系数分别为r=-0.128,r=-0.189,r=-0.207,P<0.05。结论胆红素和纤维蛋白原与糖尿病肾病的发生相关。%Objective To explore the relationship between serum bilirubin, fibrinogen (Fib) and diabetic ne-phropathy (DN) in patients with type 2 diabetes mellitus (T2DM). Methods A total of 629 T2DM patients (T2DM group) were classified into normoalbuminuria group (group A, n=474), microalbuminuria group (group B, n=100) and macroalbuminuria group (group C, n=55) according to the degree of urinary albumin excretion. We randomly chose 146 healthy adults as the control group. The difference in serum bilirubin, Fib among groups and the correlation be-tween serum bilirubin, Fib and diabetic nephropathy were analyzed

  7. THE RELATION OF BILIRUBIN AND MALONDIALDEHYDE WITH DIABETIC RETINOPATHY IN 2 DIABETES MELLITUS%血清胆红素和脂质过氧化物水平与2型糖尿病视网膜病变的相关性研究

    Institute of Scientific and Technical Information of China (English)

    伟伟; 路强

    2015-01-01

    目的::测定2型糖尿病伴或不伴糖尿病视网膜病变( diabetic retinopathy,DR)的病人血清中胆红素和脂质过氧化物( malondialdehyde,MDA)水平,探讨其与糖尿病视网膜病变严重程度的相关性。方法:研究共纳入160人。无糖尿病病人60人作为对照。其余患有2型糖尿病的病人100人作为实验组,其中有54名病人无DR,46名病人伴有DR。结果:对照组血清中总胆红素、直接胆红素、间接胆红素水平均高于实验组( P=0.016,0.032,0.023)。同时,糖尿病病人血清MDA水平高于对照组(P=0.00)。血清总胆红素、直接胆红素、间接胆红素水平较不伴DR病人血清水平低(P=0.00,0.019和0.006)。受试者基于血清总胆红素浓度,DR与胆红素的相关性在最高四分位数的胆红素病人中相比于最低四分位数组显著降低。 DR的严重程度与总胆红素水平成反比(P=0.001)。多元回归分析表明总胆红素水平与DR相关(P=0.034)。结论:糖尿病伴有DR的血清总胆红素水平较不伴有DR的糖尿病病人和对照组显著降低,而MDA水平没有显示出与DR有任何关联性。%Objective:To measure the levels of serum bilirubin and MDA in type 2 DM patients with and without diabetic retinopathy( DR) and to correlate them with severity of DR. Methods:A total number of 160 patients,60 were controls without type 2 DM and the rest 100 were type 2 DM patients were recruited in the study. The 100 diabetics, out of which 54 patients did not have DR and 46 patients had DR. Results:The three parameters,total bilirubin,direct bilirubin,indirect bilirubin were higher in controls as compared to experiment group ( P=0 . 016 , 0 . 032 , 0 . 023 ) . Serum MDA levels were shown to be higher in diabetics when compared to controls(P=0. 00). The values of all the three parameters,total bilirubin,direct bilirubin and indirect bilirubin were lower in patients with retinopathy as compared to those without retinopathy(P=0. 00

  8. Relationship between serum bilirubin and diabetic retinopathy in patients with type 2 diabetes%2型糖尿病增殖期视网膜病变与血清胆红素水平关系的研究

    Institute of Scientific and Technical Information of China (English)

    冯伟; 杨金奎

    2012-01-01

    Objective To investigate the difference of serum bilirubin between non-diabetic retinopathy( NDR) in long-standing type-2 diabetes mellitus and proliferative diabetic retinopathy( PDR) . Methods Type-2 diabetic patients who were out-patient or who attended the department of endocrinology at Capital Medical University affiliated Tongren Hospital between March 2010 and December 2011 were recruited. Of all the patients, 253 had diabetic duration longer than 10 years and non-diabetic retinopathy( NDR) (male female ratio: 136/117) , and 218 had proliferative diabetic retinopathy( PDR) and diabetic duration was not restricted( male female ratio: 98/120). Comparing the level of serum bilibubin between the two groups and the significant parameters were taken to the logistic regression formula. Results Both total bilirubin and indirect bilirubin were not significantly different between the two groups(P>0. 05) , however, patients with NDR had higher direct bilirubin than those with PDR(P<0. 05). Concerning sex, male patients in NDR group had higher direct bilirubin than those with PDR. But in female patients, no significant diffenence was found. In the logistic regression analysis about diabetic retinopathy risk factors, age and direct bilirubin were protective factors; and systolic blood pressure, HbAlc were risk factors. Conclusion The level of direct bilirubin between the NDR group with diabetic duration longer than 10 years and PDR was different. Bilirubin is a natural anti-oxidant, and can play a protective role in the onset and development of diabetic retinopathy.%目的 研究2型糖尿病长病程的无糖尿病视网膜病变患者(病程≥10年)与增殖期糖尿病视网膜病变患者之间胆红素水平的差异.方法 2010年3月至2011年12月于首都医科大学附属北京同仁医院内分泌科门诊或住院治疗的2型糖尿病患者.其中病程≥10年且无糖尿病视网膜病变(non-diabetic retinopathy,NDR)的患者253例(男女比例为136

  9. 无胆红素脑病临床表现的新生儿头颅MR改变预后分析%Prognostic analysis of cranial MR of newborn without bilirubin encephalopathy clinical manifestations

    Institute of Scientific and Technical Information of China (English)

    金许芳; 隋华

    2015-01-01

    目的:研究无胆红素脑病临床症状的新生儿,头颅MR对称性苍白球T1WI高信号改变预后情况。方法:收治新生儿30例,头颅MR有典型特征性改变,所有患者均无明显胆红素脑病典型特征性临床表现。同时排除胆道闭锁、肝炎综合征等直接胆红素升高为主的疾病及先天畸形、遗传代谢病,以及排除颅内感染、颅内出血、缺氧缺血性脑病等疾病患者。对所有患者进行随访观察、神经行为发育检查,并进行相关项目复查。结果:对上述所有患者生后1周岁内随诊观察、神经行为发育检查,神经行为发育情况基本正常。结论:头颅MRI对称性苍白球T1WI高信号改变不能作为高胆红素血症患者胆红素脑病的唯一标准,没有胆红素脑病临床表现,仅有头颅MRI改变的高胆红素血症患者一般不会有神经系统后遗症。%Objective:To study the prognosis of cranial MR symmetry pallidum T1W1 high signal change in the newborn without bilirubin encephalopathy clinical manifestations.Methods:30 newborns were selected.Their cranial MR had typical characteristic change,all patients had no clear bilirubin encephalopathy typical characteristic clinical manifestations.At the same time,it excluded biliary atresia,hepatitis syndrome and other direct bilirubin increased disease and congenital malformation,inherited metabolic diseases,and excluded intracranial infection,intracranial hemorrhage,hypoxic ischemic encephalopathy and other neurological diseases.Results:All of these patients were given follow-up observation,neurobehavioral development examination within 1 year,the neurobehavioral development situation was normal.Conclusion:The cranial MR symmetry pallidum T1W1 high signal change cannot be the only standard of bilirubin encephalopathy in patients with hyperbilirubinemia,the patients with hyperbilirubinemia have no clinical manifestations of bilirubin encephalopathy,only have

  10. Impact of misoprostol using in labor induction of prolonged pregnancy on the serum total bilirubin of neonates%应用米索前列醇引产对新生儿血清总胆红素的影响

    Institute of Scientific and Technical Information of China (English)

    李宁

    2015-01-01

    目的:探讨对超过预产期产妇应用米索前列醇引产对新生儿血清总胆红素的影响。方法选择2010年12月至2012年12月在沈阳市沈河区妇幼保健所出生的因超过预产期引产的新生儿155例,其中无药物引产(A组)50例,催产素引产(B组)52例,米索前列醇引产(C组)53例,测定3组新生儿出生后24 h、96 h微量血清总胆红素。结果3组新生儿出生后24 h、96 h微量血清总胆红素水平分别为:A组(47.3±1.8)umol/L、(173.9±5.9)umol/L,B组(55.8±2.1)umol/L、(198.9±6.1)umol/L,C组(64.2±2.7)umol/L、(230.9±6.1)umol/L。新生儿微量血清总胆红素水平A组低于B组、C组(P<0.05);A组、B组新生儿高胆红素血症发生率、新生儿窒息发生率低于C组( P<0.05)。结论米索前列醇用于引产可导致新生儿血清胆红素升高。%Objective To explore the impact of misoprostol using in labor induction of prolonged pregnancy on the serum total bilirubin of neonates .Methods 155 neonates of induced labor in Maternal and Child Health Care Institution of Shenhe District in Shenyang City from Dec.2010 to Dec.2012 were selected, Group A(n =50), Group B(n =52) and Group C(n =53) who conducted induced labor with no drug , oxytocin and misoprostol separately .The serum total bilirubin were measured for neonates 24h, 96h after birth.Results The serum total bilirubin of neonates 24h, 96h after birth of 3 groups:group A were(47.3 ±1.8)umol/L, (173.9 ±5.9)umol/L, group B were(55.8 ±2.1)umol/L,(198.9 ±6.1)umol/L, group C were(64.2 ±2.7)umol/L,( 230.9 ± 6.1)umol/L.The serum total bilirubin of neonates of group A was lower than group B and group C (P<0.05), The incidence of neonatal hyperbilirubinemia and neonatal asphyxia of group A and group B were lower than group C ( P <0.05 ) .Conclusion Misoprostol using in labor induction of prolonged pregnancy may cause a increase of serum bilirubin of

  11. 新生儿胆红素脑病的观察与护理%Observation and nursing of neonatal bilirubin encephalopathy

    Institute of Scientific and Technical Information of China (English)

    孙霞; 高霞; 李萍

    2014-01-01

    目的:探讨新生儿胆红素脑病(ABE)的临床治疗方法及护理措施。方法选取我院新生儿ABE患儿168例,所有患儿实施常规护理,其中2011年1月~2012年1月期间收治的患儿78例,设为C2组(对照组);2012年1月~2013年1月收治的患儿90例,设为C1组(观察组),对其予早期康复护理干预。C1组患儿临床病情表现稳定后,护理人员针对患儿实施听觉刺激、视觉刺激以及嗅觉刺激等系列护理。对比两组患儿的MDI(智力发育指数)、PDI(运动发育指数)及智能情况。结果 C1组患儿每一时间段PDI及MDI与C2组患儿比较改善显著,差异有统计学意义(P<0.05);C1组智力异常率明显低于C2组,差异有统计学意义(P<0.05)。结论针对新生儿ABE患者予早期康复护理干预,可显著促进患儿的运动发育及智力发育,明显改善患儿的预后。%Objective To investigate the clinical treatment of neonatal bilirubin encephalopathy (ABE)and the specif-ic care measures. Methods Selected 168 cases of ABE in our hospital,and 78 cases of children admitted from January 2011 to January 2012,implementation of routine care for all children,set C2 group(control group); 90 cases of chil-dren admitted. C1 group (observation group)was from January 2012 to January 2013. Given children with early reha-bilitation nursing intervention. After clinical condition was stable in C1 group of children, caregivers for children with auditory stimuli implemented, visual stimulation and olfactory stimuli, such as a series of measures to care for the children. C1 and C2 group of children with MDI,PDI and intelligent case of children were compared. Results C1 group of children in each time period PDI and MDI,and C2 groups of children were compared,both showed significant im-provement(P<0.05);In mental abnormality rate,C1 group was lower than the C2 group(P<0.05). Conclusion ABE for neonatal patients given early rehabilitation nursing intervention can

  12. Significance to determine the serum biliru-bin in patients with acute atherosclerotic cerebral infarction%急性动脉粥样硬化性脑梗死患者血清胆红素变化的临床意义

    Institute of Scientific and Technical Information of China (English)

    赵守财; 储照虎; 马领松; 陈奕农; 汪飞

    2013-01-01

    Objective:To determine the serum bilirubin variation in pa-tients with acute atherosclerotic cerebral infraction ( ACI) for ascertaining whether bilirubin can be neuro-protective role in acute stroke patients . Methods:The risk factors and imaging information were collected in ACI patients for calculation of the infract size and quantitative measure of the stroke-related neurologic deficit by National Institutes of Health Stroke Scale(NIHSS).Enzyme linked immunosorbent assays(ELISA)was per-formed to detect the levels of total bilirubin and conjugated bilirubin 24 hour after admission.The bilirubin ratio(quotient of between direct biliru-bin and the total bilirubin)were calculated,and the relationship was esti-mated on the risk factors basis regarding the infract size,NHHSS scoring and the bilirubin ratio.Results:The levels total bilirubin and direct biliru-bin were in normal limit and showed no significant difference for ACI pa-tients and control subjects,and the mean ratio of bilirubin was 0.327 ± 0.063 in ACI patients.Logistic regression analysis on dependent variable of bilirubin suggested no significant difference concerning remaining risk factors except for statistical significance being observed relative to biliru-bin ratio,alcohol drinking patterns and history of coronary artery disease. Subgrouping by bilirubin ratio as 0.20-0.29,0.30 -0.34 and 0.35 -0.51 in ACI patients showed that those with bilirubin ratio of 0.20 -0.29,0.30-0.34 were significantly different,whereas those with biliru-bin ratio of 0.35 -0.51 had no statistical difference before and after medication.Conclusion:Although the serum bilirubin levels may remain in normal limit in patients with ACI , yet the ratio variation of bilirubin seems associated with the severity of this entity .%目的:了解急性动脉粥样硬化性脑梗死患者血液中胆红素的变化,探讨胆红素的脑保护作用。方法:通过收集患者各危险因素及影像学资料,计算梗死面积,

  13. The clinical application of serum albumin and unconjugated bilirubin de-tection in neonatal jaundice%血清白蛋白、非结合胆红素的检测在新生儿黄疸中的临床应用

    Institute of Scientific and Technical Information of China (English)

    曹丽琴

    2015-01-01

    Objective To study the clinical application value of serum albumin and unconjugated bilirubin detection in neonatal jaundice. Methods A total of 86 cases of neonatal jaundice were enrolled in this study. The serum albumin was measured by ELISA analysis. The unconjugated bilirubin was measured by vanadate oxidation analysis. Results There were significant differences of serum albumin, unconjugated bilirubin, and total bilirubin between moderate jaun-dice group and severe jaundice group(P<0.05﹚. The expressions of serum albumin, unconjugated bilirubin, and total biliru-bin were significant differences among infection, hemolytic, and obstructive jaundice group (P<0.05﹚. Conclusion The detection of serum albumin,unconjugated bilirubin and and total bilirubin has something to contribute to diagnose, exti-mate pathogenetic condition, and analyze etiological factor in neonatal jaundice.%目的:检测新生儿黄疸白蛋白和非结合胆红素表达的变化。方法收集我院收治的新生儿黄疸患儿86例,血清白蛋白使用ELISA法测定,非结合胆红素使用钒酸盐氧化法测定。结果白蛋白、非结合胆红素和总胆红素的表达水平在病理性黄疸中度组和重度组之间具有显著性差异(P<0.05﹚。感染性、溶血性和阻塞性黄疸组之间上述指标也存在显著性差异(P<0.05﹚。结论血清白蛋白、非结合胆红素以及总胆红素水平的检测有助于新生儿黄疸的诊断、评估病情以及病因的分析。

  14. 高效液相色谱法测定“天竺痫康宁胶囊”中胆红素的含量%Determination of bilirubin in Tianzhu Xiankangning capsules by HPLC

    Institute of Scientific and Technical Information of China (English)

    张颖; 宋志钊; 刘元

    2016-01-01

    目的:建立天竺痫康宁胶囊胆红素含量的高效液相色谱(HPLC)测定方法。方法采用 Intersil -C18色谱柱,流动相为甲醇-三氯甲烷-1%磷酸(80∶14∶6),流速1.0 mL·min -1,检测波长为450 nm,柱温为室温,进样量为10μL。结果在该条件下,浓度为2.26~45.2 mg·L -1范围内,胆红素峰面积与其浓度呈良好线性关系(r =0.9996),平均回收率为97.4%,RSD为2.02%(n =6)。结论该方法灵敏、简便、准确、重复性好,可作为天竺痫康宁胶囊质量标准的控制指标之一。%Objective To establish an HPLC method for the determination of bilirubin in Tianzhu Xiankangning capsules.Methods The Intersil -C18 column was used,and the mobile phase consisted of Methanol -trichloromethan -1% phosphoric acid (80∶14∶6) with the flow rate at 1.0 mL·min -1 .The detection wave length was 450 nm and the sample size was 10 μL.Results Linearity of bili-rubin was good around 2.26 ~45.2 mg·L -1 ,r =0.999 6.The average recovery was 97.4% with RSD less than 2.02% (n =6). Conclusions The method is sensitive,simple,accurate and repeatable,which can be applied to the determination of bilirubin in Tianzhu Xiankangning capsules.

  15. Prediction of neonatal hyperbilirubinemia by transcutaneous bilirubin nomograms%新生儿高胆红素血症风险的预测

    Institute of Scientific and Technical Information of China (English)

    陈兰; 石碧珍; 韩树萍; 陈超; 刘玲

    2016-01-01

    目的 绘制本地区新生儿经皮胆红素(transcutaneous bilirubin,TcB)百分位列线图,结合临床风险因素预测新生儿高胆红素血症的发生风险. 方法 研究对象为2013年1月1日至12月31日在贵州省妇幼保健院产科出生的健康足月儿和晚期早产儿(胎龄≥35周,出生体重≥2 000 g).连续记录这些新生儿生后168 h内的TcB值.将生后72 h内对应的危险区TcB测定值作为预测指标,采用小时TcB百分位列线图,结合受试者工作特性(receiver operative characteristic,ROC)曲线及Logistic回归模型,评价小时TcB百分位列线图对新生儿高胆红素血症的预测价值.采用分类数据关联性分析、Pearson x2检验或Logistic回归模型,对数据进行统计学分析. 结果 共5 250例新生儿纳入本研究.在生后40 h内,TcB值快速上升,40~96 h上升速度较前有所减缓,96 h后维持在较高水平.其中,TcB第95百分位数在96 h已趋于稳定.本研究中,第40、75和95百分位TcB峰值分别为173、217和248 μmol/L.5 250例新生儿中,277例(5.3%)处于72 h内高危区,阳性预测值为22.02%;1 087例(20.7%)和1 854例(35.3%)分别处于72 h内中高危区和中低危区,阳性预测值分为10.58%和3.72%;2032例(38.7%)处于72 h内低危区,阳性预测值为1.38%.多因素分析结果显示,72 h后TcB高危险区与胎龄、分娩方式、喂养方式和72 h内危险区水平有关联.与胎龄≥40周的新生儿相比,胎龄≥37~<40周者更有可能处于高危区(OR=1.80,95%CI:1.29~2.51);与经阴道分娩的新生儿相比,剖宫产出生的新生儿处于高危区的可能性可降低42%;与人工喂养的新生儿相比,混合喂养(母乳为主)的新生儿处于高危区的可能性降低(OR=0.51,95%CI:0.29~0.88);随着72 h内TcB危险区水平的降低,研究对象72 h后处于高危区的可能性逐渐降低.ROC曲线分析显示,72 h内预测新生儿高胆红素血症发生风险的曲线下面积为0

  16. The study of the correlation between the serum bilirubin level and the intracranial artery stenosis of the patients with cere-bral infarction%脑梗死患者血清胆红素含量与颅内动脉狭窄的相关性研究

    Institute of Scientific and Technical Information of China (English)

    王鸫霖

    2014-01-01

    Objective To analyze the correlation between the serum bilirubin level and the intracranial artery steno-sis of the patients with cerebral infarction by using DSA to observe the intracranial artery stenosis of the patients with cerebral infarction.Methods 200 patients with cerebral infarction were included as qualified cases.100 patients with mild stenosis or without stenosis were included into the control group.The research objects were divided into two groups according to whether there was the intracranial artery stenosis or not.At the same time,the subgroup analysis was employed to the degrees of stenosis.The differences and changes of the serum bilirubin levels in the different groups and subgroups were compared.The regression analysis on the related risk factors was employed.Results There were statistically significant differences of the total bilirubin SIB,the indirect bilirubin STB between the stenosis group and the control group(P <0.05).The total bilirubin SIB and the indirect bilirubin STB of the stenosis group were sig-nificantly lower than those of the control group.The total bilirubin SIB and the indirect bilirubin STB of the severe ste-nosis and the occlusion group were significantly lower than those of the moderate stenosis group.Multivariate regres-sion analysis showed that serum bilirubin,hypertension,diabetes related to intracranial artery stenosis.Conclusion A lower serum bilirubin level may be an important risk factor of cerebral infarction and correlate with the occurrence of in-tracranial atherosclerotic stenosis.%目的:采用 DSA 观察脑梗死患者颅内动脉狭窄情况,深入分析血清胆红素含量与与患者颅内动脉狭窄的相关性。方法纳入合格的脑梗死患者200例。对照组为无狭窄或轻度狭窄患者,例数为100例。根据颅内动脉有无狭窄分成两组。同时进行狭窄程度的亚组分析。比较不同组间及其亚组内的血清胆红素含量的差异与变化。对相关危险

  17. The Value Analysis in Clinical Test of Serum Bilirubin and Uric Acid in Patients with Coronary Heart Disease(CHD)%血清胆红素与尿酸在冠心病患者临床检验中的价值分析

    Institute of Scientific and Technical Information of China (English)

    欧阳莉娜

    2013-01-01

    目的:对血清胆红素与尿酸在冠心病患者临床检验中的价值进行评价分析,为今后的临床诊治工作提供可靠的参考依据。方法:抽取在2010年7月-2013年7月本院收治的冠心病临床患者78例和同期健康体检者60例,对其展开血清胆红素与尿酸水平检测,并对比分析两组研究对象的检测结果。对冠心病患者行冠脉造影检测,计算Gensini积分,对比分析不同积分者的各项观察指标的检测结果。结果:冠心病组患者血清总胆红素、直接胆红素、间接胆红素水平均较对照组发生显著降低(P<0.05),血清尿酸水平较对照组发生显著升高,比较差异均有统计学意义(P<0.05)。随着Gensini积分的增加血清总胆红素、直接胆红素、间接胆红素水平逐渐降低,而尿酸水平则逐渐增加。结论:对血清胆红素、尿酸水平进行检测可对冠心病的诊断、冠状动脉狭窄程度评估等提供可靠的参考依据,值得关注。%Objective:To evaluate and analysis the clinical trial value of serum bilirubin and uric acid in patients with coronary heart disease. To provide reliable reference for clinical diagnosis and treatment work in the future.Method:78 clinical physical examination patients with coronary heart disease and 60 healthy patients were selected from our hospital between from July 2010 to July 2013,their serum bilirubin and uric acid level were detection,and two groups of test results were compared;the line of coronary angiography in patients with coronary heart disease was detection,to sum the Gensini score.The different between the observation indexes of the integral test results were compared and analyzed.Result:The serum total bilirubin, direct bilirubin and indirect bilirubin levels of the coronary artery disease patients were significantly lower than the control group decreased(P<0.05), the serum uric acid level was significantly higher,there were

  18. Conjugated Bilirubin Differentially Regulates CD4+ T Effector Cells and T Regulatory Cell Function through Outside-In and Inside-Out Mechanisms: The Effects of HAV Cell Surface Receptor and Intracellular Signaling

    Science.gov (United States)

    Corral-Jara, Karla F.; Gómez-Leyva, Juan F.; Rosenstein, Yvonne; Jose-Abrego, Alexis; Roman, Sonia

    2016-01-01

    We recently reported an immune-modulatory role of conjugated bilirubin (CB) in hepatitis A virus (HAV) infection. During this infection the immune response relies on CD4+ T lymphocytes (TLs) and it may be affected by the interaction of HAV with its cellular receptor (HAVCR1/TIM-1) on T cell surface. How CB might affect T cell function during HAV infection remains to be elucidated. Herein, in vitro stimulation of CD4+ TLs from healthy donors with CB resulted in a decrease in the degree of intracellular tyrosine phosphorylation and an increase in the activity of T regulatory cells (Tregs) expressing HAVCR1/TIM-1. A comparison between CD4+ TLs from healthy donors and HAV-infected patients revealed changes in the TCR signaling pathway relative to changes in CB levels. The proportion of CD4+CD25+ TLs increased in patients with low CB serum levels and an increase in the percentage of Tregs expressing HAVCR1/TIM-1 was found in HAV-infected patients relative to controls. A low frequency of 157insMTTTVP insertion in the viral receptor gene HAVCR1/TIM-1 was found in patients and controls. Our data revealed that, during HAV infection, CB differentially regulates CD4+ TLs and Tregs functions by modulating intracellular pathways and by inducing changes in the proportion of Tregs expressing HAVCR1/TIM-1. PMID:27578921

  19. Relationship between the serum bilirubin level and the severity of disease and short -term outcome in patients w ith ischemic stroke%急性缺血性卒中患者血清胆红素水平与病情严重程度和短期转归的关系

    Institute of Scientific and Technical Information of China (English)

    陈国栋; 肖瑾; 刘宾荣; 戴健; 王峰; 吴志勇; 储照虎

    2016-01-01

    Objective To investigate the relationship betw een the serum bilirubin level and the severity of disease and short-term outcome in patient w ith acute ischemic stroke. Methods A total of 120 consecutive inpatients w ith acute ischemic stroke w ere enroled and 105 healthy subjects at the same time w ere used as a control group. The biochemical indicators, such as serum total bilirubin, direct bilirubin, indirect bilirubin, blood lipid, and blood glucose w ere measured w ithin 24 h after admission. The National Institutes of Health Stroke Scale ( NIHSS ) w as used to assess the neurological deficits on the day of admission. The NIHSS score 2 w as defined as poor outcome. The levels of serum total bilirubin, direct bilirubin, and indirect bilirubin w ere measured again. Results The levels of serum total bilirubin, direct bilirubin, and indirect bilirubin in the moderate to severe stroke group w ere significantly higher than those in the mild stroke group ( P 0.05). Conclusions The serum bilirubin level show ed stress increase in patients w ith cerebral infarction in acute phase; and it w as significantly associated w ith the degree of neurological deficit, but it w as not associated w ith short-term outcome. It might be a defense response to the body for stroke events.%目的:探讨急性缺血性卒中患者血清胆红素水平与病情严重程度和短期转归的关系。方法纳入连续的120例急性缺血性卒中住院患者,105例同期健康体检者作为对照组。在入院24 h内检测血清总胆红素、直接胆红素、间接胆红素以及血脂、血糖等生化指标,入院当天采用美国国立卫生研究院卒中量表(National Institutes of Health Stroke Scale, NIHSS )评定神经功能缺损情况,NIHSS评分2分定义为转归不良,并再次检测血清总胆红素、直接胆红素和间接胆红素水平。结果中重度卒中组血清总胆红素、直接胆红素和间接胆红素水平均显著高于轻

  20. Correlation and Clinical Significance of Serum Bilirubin Level and TIMI Risk Score in Patients with Non-ST Segment Elevation Myocardial Infarction%非ST段抬高型心肌梗死患者血清胆红素水平与TIMI危险评分的相关性及临床意义

    Institute of Scientific and Technical Information of China (English)

    王涛

    2016-01-01

    目的:探讨非 ST段抬高型心肌梗死(NSTEMI)患者血清胆红素(BIL)水平与TIMI评分的相关性及临床意义。方法收集104例 NSTEMI患者一般资料,包括年龄、性别、体质量指数、血压、糖尿病、低密度脂蛋白胆固醇水平以及吸烟等,清晨空腹采血测定血清总胆红素(TBIL)、直接胆红素(DBIL)、间接胆红素(IBIL)水平。采用TIMI评分标准对NSTEMI患者进行风险评估,计算NSTEMI组患者TIMI评分并进行危险分层,以评分差异分组,分为低位组、中危组、高危组;分析 NSTEMI患者血清 BIL水平与 TIMI积分的相关性及临床意义。结果高危组患者TBIL、IBIL水平明显低于中危组、低危组(P<0.01);NSTE-MI组TBIL、IBIL水平与其TIMI分值均有负相关关系(r=-0.20,P<0.05;r=-0.228,P<0.05)。结论NSTEMI 患者血清BIL 水平与TIMI 积分呈负相关,TBIL 对NSTEMI 患者早期危险度分层具有预测价值。%Objective To investigate the correlation and clinical significance of serum bilirubin(BIL)lev-el and TIMI score in patients with non-ST segment elevation myocardial infarction(NSTEMI).Methods The clinical data of 104 patients with NSTEMI patients were collected,including age,gender,body mass in-dex,blood pressure,diabetes,low-density lipoprotein cholesterol(LDL-C)levels and smoking,and the morn-ing fasting blood determination of serum total bilirubin(TBIL),direct bilirubin(DBIL)and indirect bilirubin (IBIL).The TIMI risk score was used for risk assessment of patients with NSTEMI.TIMI scores of NSTEMI patients were calculated and their risks were stratified.Based on score differences,the patients were divided into low-risk group,middle-risk group and high-risk group.The correlation of serum bilirubin levels and TIMI risk score was analyzed as well as its clinical significance.Results 1.TIMI risk stratifica-tion showed that the levels of serum total bilirubin(TBIL)and indirect

  1. Gilbert's syndrome: High frequency of the (TA)7 TAA allele in India and its interaction with a novel CAT insertion in promoter of the gene for bilirubin UDP-glucuronosyltransferase 1 gene

    Institute of Scientific and Technical Information of China (English)

    Shabana Farheen; Sanghamitra Sengupta; Amal Santra; Suparna Pal; Gopal Krishna Dhali; Meenakshi Chakravorty; Partha P Majumder; Abhijit Chowdhury

    2006-01-01

    AIM: To identify the variants in UDP-glucuronosyltransferase 1 (UGT1A1) gene in Gilbert's syndrome (GS) and to estimate the association between homozygosity for TA insertion and GS in India, as well as the frequency of TA insertion and its impact among normal controls in India.METHODS: Ninety-five GS cases and 95 normal controls were selected. Liver function and other tests were done. The promoter and all 5 exons of UGT1A1 gene were resequenced. Functional assessment of a novel trinucleotide insertion was done byin silico analysis and by estimating UGT1A1 promoter activity carried out by luciferase reporter assay of appropriate constructs in Hep G2 cell line.RESULTS: Among the GS patients, 80% were homozygous for the TA insertion, which was several-fold higher than reports from other ethnic groups. The mean UCB level was elevated among individuals with only one copy of this insertion, which was not significantly different from those with two copies. Many new DNA variants in UGT1A1 gene were discovered, including a trinucleotide (CAT) insertion in the promoter found in a subset (10%) of GS patients, but not among normal controls. In-silico analysis showed marked changes in the DNA-folding of the promoter and functional analysis showed a 20-fold reduction in transcription efficiency of UGT1A1 gene resulting from this insertion, thereby significantly elevating the UCB level.CONCLUSION: The genetic epidemiology of GS is variable across ethnic groups and the epistatic interactions among UGT1A1 promoter variants modulate bilirubin glucuronidation.

  2. Reason analysis and solution method of internal quality total bilirubin drift%关于室内质控总胆红素漂移的原因分析及解决办法

    Institute of Scientific and Technical Information of China (English)

    郑风林

    2015-01-01

    Objective:To study the reason and solution of the down drift of total bilirubin serum prepared monthly. Methods:The prepared quality-control serum was distributed into 30 crystal or brown bullet-shaped tubes respectively, and stored at-20℃. Take out a tube every day for test.Results:The test level of quality-control serum preserved in crystal tubes drift down obviously everyday,while that in brown tubes was stable in a desired range. Conclusions:The down-drift of quality-control serum levels could be prevented effectively by preserved in brown tubes.%目的:研究月配质控血清总胆红素(TBIL)测定值随着时间的推移向下漂移的原因及找出解决的办法。方法:把配制的质控血清分别分装在30支普通透明的子弹头状离心管和30支棕褐色避光的离心管中放冰箱冷冻备用,每天取出一支进行质控检测。结果:普通透明离心管保存的质控血清TBIL测定值逐天明显向下漂移,而避光保存的质控血清其TBIL测定值则基本稳定在一个理想的质控允许范围以内。结论:用棕褐色离心管保存质控血清,可有效防止质控血清TBIL测定值的向下漂移。

  3. Apoptosis and impairment of neurite network by short exposure of immature rat cortical neurons to unconjugated bilirubin increase with cell differentiation and are additionally enhanced by an inflammatory stimulus.

    Science.gov (United States)

    Falcão, Ana S; Silva, Rui F M; Pancadas, Sérgio; Fernandes, Adelaide; Brito, Maria A; Brites, Dora

    2007-05-01

    Nerve cell injury induced by unconjugated bilirubin (UCB) has been implicated in brain damage during severe neonatal hyperbilirubinemia, although the molecular mechanisms underlying UCB neurotoxicity are still not clarified. It has been suggested recently that there is an association between hyperbilirubinemia and long-term neurologic dysfunctions. We incubated immature neurons with UCB to evaluate the short- and long-term effects of UCB on apoptotic death and on neuritic outgrowth and ramification. We also evaluated whether mature neurons, exposed previously to UCB in an early stage of differentiation, are more sensitive to apoptosis or to neuritic breakdown when treated with inflammatory agents, such as lipopolysaccharide and tumor necrosis factor-alpha. Results show that exposure of immature neurons to UCB increased apoptosis and provoked a reduction of both neurite extension and number of nodes. These injurious effects observed in immature cells treated with UCB were increasingly perpetuated along cell differentiation, as compared to neurons incubated in the absence of UCB. In addition, neurons that were exposed to UCB when immature showed an increased susceptibility to death by apoptosis, as well as an additional decrease in neurite outgrowth when incubated with an inflammatory agent afterward. This work shows, for the first time, that UCB induces neurite changes consistent with neurodevelopment abnormalities. Furthermore, pre-exposure to UCB followed by an inflammatory stimulus leads to an enhanced susceptibility to long-term apoptosis, as well as a greater neuritic breakdown. These data support the association between neonatal hyperbilirubinemia and the later development of mental illness, such as schizophrenia.

  4. 64 cases of neonatal acute bilirubin encephalopathy in the early clinical observation of amplitude-integrated electroencephalography change%64例新生儿急性胆红素脑病早期改变时振幅整合脑电图的临床观察

    Institute of Scientific and Technical Information of China (English)

    段玉会; 王洲洪; 苏萍; 韦巧珍

    2014-01-01

    目的:通过对新生儿高胆红素血症患儿持续脑功能监测,探讨新生儿胆红素脑病早期改变时振幅整合脑电图(amplitude-integrated electroencephalography a EEG)变化,为判断预后提供一定的参考。方法选择2012年1月-2013年6月在两家医院新生儿科收治64例胆红素水平≥320umol/l但无明显临床症状的足月儿,在入院后抽取血清查胆红素水平同时行脑功能监测,并与脑干诱发电位(Brainstem Auditory Evoked Potential,BAEP)结果比较。结果aEEG异常42例,异常率为65.6%,其中轻度异常32例(66.6%),表现为背景不连续或连续正常电压合并单次惊厥;重度异常10例(23.8%),表现为背景波不连续,伴惊厥发作。64例患儿均行干诱发电位检查, aEEG轻度异常32例中BAEP正常4例,轻度异常25例,中度异常3例;aEEG重度异常10例中BAEP中度异常6例,重度异常4例。结论振幅整合脑电图可反应新生儿胆红素脑病的早期脑损伤变化,并与BAEP异常分级存在相关性;可作为急性胆红素脑病早期诊断依据。%Objective Through to the newborn children with high blood bilirubin brain function monitoring continuously,to observe the change of early neonatal bilirubin encephalopathy when amplitude-integrated electroencephalography (aEEG)changes tor Provide certain reference for prognosis. Methods Choice in January 2012 - June 2013 in two neonatal department treated 64 cases of bilirubin levels of 320 umol/l, but no clinical symptoms ful- term infant .Extract serum after admission blood bilirubin level lines of brain function monitoring at the same time, And compared with Brainstem Auditory Evoked Potential(BAEP).Results Abnormal aEEG 42 cases, the abnormal rate was 65.6%, the mild abnormal 32 cases(66.6%), showed the background discrete or continuous normal voltage combined single convulsions; and severely abnormal 10 cases(23.8%), showed the background waves of

  5. 评价血脂、血尿酸、胆红素、纤维蛋白原与冠心病的相关性研究%The correlation research of blood lipid, bilirubin, UA and fibrinogen with CHD

    Institute of Scientific and Technical Information of China (English)

    贾向东; 郭长城

    2014-01-01

    目的 探讨冠心病(coronary heart disease,CHD)患者的血脂、胆红素、血尿酸(uric acid,UA)及纤维蛋白原(fibrinogen,Fib)的水平变化及其与CHD的相关性.方法 选择2013年10月至2014年1月我院120例经冠脉造影确诊的CHD患者(CHD组)及100例同期健康体检者(正常对照组)为研究对象,分别检测两组受检者的血清总胆固醇(total cholesterol,TC)、甘油三酯(triglyceride,TG)、高密度脂蛋白胆固醇(high density lipoprotein cholesterol,HDL-C)、低密度脂蛋白胆固醇(low density lipoprotein cholesterol,LDL-C)、载脂蛋白AI (apoprotein AI,Apo AI)、载脂蛋白B(apoprotein B,Apo B)、UA、总胆红素(total bilirubin,TBIL)、直接胆红素(direct bilirubin,DBIL)、间接胆红素(indirect bilirubin,IBIL)及Fib水平,对检测结果进行统计学分析.结果 CHD组的TC、TG、LDL-C、Apo B、UA、Fib均高于正常对照组,且差异均有统计学意义(P均< 0.05).CHD组的HDL-C、Apo AI、TBIL、DBIL、IBIL均低于正常对照组,且差异均具有统计学意义(P均< 0.05).结论 CHD的发病与TC、TG、UA、Fib和胆红素水平具有相关性,是CHD的危险因素.

  6. 药浴水疗对早产儿及低体重儿胆红素及血糖的影响%The effects of medicated hydrotherapy on bilirubin and blood glucose in premature and low birth weight neonates

    Institute of Scientific and Technical Information of China (English)

    曾小华; 朱湘陵; 冯兰青; 卢群娣; 曹辉娟; 叶惠梅; 陈婉婷

    2012-01-01

    目的 根据新生儿黄疸药浴方制成适合早产儿及低体重儿的药浴水疗方,观察此药浴水疗方对早产儿及低体重儿黄疸及血糖的影响.方法 选择住院的采用药浴水疗的38例早产儿或低体重儿为药浴组,同期非药浴水疗的38例早产儿或低体重儿为对照组,以新生儿黄疸的药浴方加减制成适合早产儿或低体重儿生理特点的药浴方,配成500 mL的药液,两组均在出生后24 h进行水疗或沐浴,监测其血糖值、经皮胆红素值的变化.结果 (1)药浴组药浴前后血糖比较,第1、3天差异有统计学意义(P0.05);(2)两组胆红素值比较:第24、48小时两组差异无统计学意义(P>0.05),出生后第96、144小时差异有统计学意义(P 0.05). There was no significant difference of bilirubin between two groups at the 24th and 48 th hour after birth (P > 0. 05 ) ; while it was significantly lower in hydrotherapy group than that in control group at the 96th and 144th hour (P < 0.01). Conclusion The medicated hydrotherapy can reduce hyperbilirubinemia in premature infants and low birth weight infants within 24 hours after birth and alleviate physiological jaundice . And there is no hypoglycemia in medicated bathing.

  7. 血清神经元特异性烯醇化酶和胆红素与大动脉粥样硬化性卒中后脑功能损伤及预后的关系%Relationship between serum neuron-specific enolase,bilirubin and cerebral dysfunction,prognosis after large-artery atherosclerotic stroke

    Institute of Scientific and Technical Information of China (English)

    王岩; 欧阳和中; 刘凤国; 刘淑艳; 谢莉

    2015-01-01

    Objective Toinvestigatetherelationbetweenserumneuron-specificenolase(NSE), bilirubinandcerebraldysfunction,prognosisafterlarge-arteryatheroscleroticstroke.Methods According to the Trial of Org 10172 in Acute Stroke Treatment (TOAST)criteria,all the 73 patients with large artery atherosclerotic stroke were divided into the test group (41 cases ) and control group (32 cases ) according to the elevated or normal levels of serum NSE and total bilirubin. At the first day of their hospitalization,the National Institutes of Health Stroke Scale (NIHSS)score was conducted,their serum NSE,bilirubin (total bilirubin,direct bilirubin,indirect bilirubin)levels were detected,and were compared with the reevaluation of 7 and 14 days of their hospitalization and reexamination results. The modified Rankin Scale (mRS)was use to assess the recovery of their neurological function at day 30 after onset/admission. The prognosis of the patients was followed up at 1 year after onset/admission. The Kaplan-Meier product-limit method was used to conduct the analysis of the good outcome rate,and the good outcomes of both groups/interlayers (different bilirubin and NSE levels)were tested with Log-rank test. Results (1)The NIHSS scores,the levels of serum bilirubin and NSE at day 1,7,and 14 in the test group were significantly higher than those of a control group (all P<0. 01). The levels of serum bilirubin and NSE at day 7 and 14 were lower than those at day 1. (2)The mRS score at day 30 between the test group and the control group was singnificantly different (Z =3. 286,P =0. 001). (3)At day 1,the CT detection rate of large area cerebral infarction of the test group was significantly higher than that of the control group (56. 1%[n=23]vs. 28. 1%[n=9]). There was significant difference (χ2 =5. 712,P=0. 017). (4)The analysis result of Kaplan-Meier showed that there was no significant difference in its good outcome no matter grouped by the test or by serum NSE level stratification of the

  8. 老年冠心病患者冠状动脉狭窄程度与血清胆红素水平的相关性%Relationship between serum level of bilirubin and extent of coronary artery stenosis in aged patients with coronary heart disease

    Institute of Scientific and Technical Information of China (English)

    张晓艳; 安映红; 魏璇; 李明阳

    2012-01-01

    Objective: To investigate relationship between serum level of bilirubin and extent of coronary artery stenosis in aged patients with cardiovascular diseases and its significance. Methods: A total of 264 aged patients with coronary heart disease (CHD) underwent coronary angiography and measurement of serum level of bilirubin. According to results of coronary angiography, patients were divided into normal control group (n= 68) and CHD group (n = 196), and CHD group was further divided into mild group (n = 66), moderate group (n = 64) and severe group (n = 66). Results: One-factor ANOVA analysis indicated compared with normal control group, the serum levels of total bilirubin [ (20. 52 ± 6. 12) μmol/L vs. (18. 07 ± 5. 81)μmol/L vs. (14. 22 ± 5. 19) mol/L vs. (15. 63 ± 6. 30) μmol/L] and indirect bilirubin [ (18. 80 ± 5. 21) μmol/L vs. (15. 46 ± 6. 71)μmol/L vs. (11. 63 ± 3. 42) μmol/L vs. (13. 37 + 4. 35) μmol/L] significantly decreased in mild CHD group, moderate CHD group and severe CHD group (P<0. 05~<0. 01); the multiple linear regression analysis indicated that serum levels of total bilirubin and indirect bilirubin were significant negative correlations with scores of coronary artery stenosis (partial correlation coefficients were -8. 976 and -2. 492 respectively, P<0. 05). Conclusion: Level of bilirubin is closely related with incidence, development and severity of cardiovascular diseases in aged patients, total and indirect bilirubin can be used for good indicators for predicting severity and range of lesions in coronary artery disease.%目的:探讨老年心血管病患者的冠脉狭窄程度与血清胆红素水平的关系及意义.方法:对264例老年冠心病患者行冠脉造影并测定血清胆红素水平.依据冠脉造影结果,患者被分为正常对照组(68例)和冠心病组(196例),其中冠心病组又分为轻度组(66例)、中度组(64例)和重度组(66例).结果:单因素方差分析显示,与正常对照组比较,冠

  9. Adsorption Effect of AmberliteXAD-4 Resin Based Calix[4]arene on Bilirubin in Aqueous Medium%杯【4】芳烃修饰Amberlite XAD-4树脂对水介质中胆红素的吸附性能

    Institute of Scientific and Technical Information of China (English)

    熊振湖; 朱乐; 王月

    2011-01-01

    通过偶氮化反应将杯【4】芳烃接枝到AmberliteXAD-4树脂上,采用FTIR、SEM等方法,表征了杯【4】芳烃修饰AmbertiteXAD-4树脂的结构.吸附实验表明,杯【4】芳烃修饰AmberliteXAD-4树脂对胆红素的吸附容量远远大于单纯AmberliteXAD-4树脂.在温度为25℃,胆红素浓度为20mg/L、吸附时间为2h的条件下,胆红素的平衡去除率在90%以上;而相同条件下的单纯AmberliteXAD-4树脂对胆红素的去除率仅为32.8%.水溶液中修饰树脂达到吸附平衡的时间为2h左右,而且温度越高达到平衡吸附的时间越短.在近中性(pH=5~7)条件下修饰树脂对胆红素的吸附容量达到最大值,为2.1medg,但随pH的降低或增大吸附容量均迅速降低.%The calix[4]arene is connected to Amberlite XAD-4 resin covalently through a diazotization reaction. The structure of Amberlite XAD-4 resin based on calix[4]arene is characterized using FT-IR, SEM. The sorption results show that Amberlite XAD-4 resin based on calix[4]arene has much better removal to the bilirubin in aqueous solutions than Amberlite XAD-4 resin which are presence alone in aqueous solutions. The removal rate of bilirubin is over 90% at equilibrium, at the conditions of 25℃, the concentration of bilirubin is 20 mg/L and the adsorption time is 2 h. At the same conditions, the removal of bilirubin from Amberlite XAD-4 resin is 2.8% alone. The equilibrium time is 2h and decreases with an increase in temperature. When the aqueous solution stays near the neutral (pH = 5 - 7), the adsorption capacity of Amberlite XAD-4 resin modified with calix[4]arene to the bilirubin reaches maximum value, which is 2.1 mg/g but the value decreases rapidly when the pH increases or decreases

  10. Investigation on the interference of bilirubin and hemoglobin for different urinary albumin measurements%胆红素和血红蛋白对不同尿白蛋白检测方法结果干扰的研究

    Institute of Scientific and Technical Information of China (English)

    刘蕊; 李刚; 崔小璠

    2012-01-01

    目的 探讨尿中胆红素(Bil)和血红蛋白(Hb)在不同检测方法中对尿白蛋白检测结果的干扰.方法 分别以Bil和Hb作为干扰物,对3种不同检测方法(免疫透射比浊法、免疫散射比浊法和免疫胶体金法)进行尿白蛋白检测的干扰试验.结果 不同浓度的Bil和Hb对3种尿白蛋白检测方法的结果均会产生影响,且干扰率会随干扰物浓度的增高而增加.Hb对3种检测方法主要呈负干扰;Bil对免疫透射比浊法和免疫散射比浊法检测结果呈正干扰,而对免疫胶体金法检测结果呈负干扰.当尿液中Hb浓度相当于尿隐血“1+”时,Hb对3种检测方法的干扰率绝对值为6.72% ~ 17.43%;当尿液中Bil浓度相当于Bil“1+”时,3种检测方法的干扰率绝对值为7.89%~ 22.94%.结论 Bil和Hb均会对尿白蛋白的检测结果造成干扰,在检测可能含有Bil和Hb患者尿液的尿白蛋白时,应考虑这些干扰因素对结果的影响.%Objective To investigate the interference of bilirubin ( Bil) and hemoglobin ( Hb) in urine on different urinary albumin measurements. Methods Bil and Hb, as interferents, performed interference test by 3 different urinary albumin measurements: immuno-nephelometry, immuno-turbidimetry and immuno-colloidal gold method, respectively. Results Bil and Hb with different concentrations could affect the results of urinary albumin by all the 3 measurements, and the interferents rate increased with the increasing concentration of interferents. Hb mainly generated negative interference by the 3 methods, while the interferences of Bil by immuno-nephelometry and immuno-turbidimetry were positive, and by immuno-colloidal gold method was negative. In the 3 methods, when urine occult blood was " 1 + " , the absolute value of interference rate of Hb was 6. 72% -17. 43% . When urine Bil was " 1 + " , the absolute value of interference rate was 7. 89%-22. 94%. Conclusions Bil and Hb could generate interference on

  11. 体检人群成人血清总胆红素浓度分布及参考区间的临床研究%Study on distribution and reference interval of serum bilirubin in physical examination population

    Institute of Scientific and Technical Information of China (English)

    刘安楠; 朱玲

    2013-01-01

    Objective To select health individual and test serum total bilirubin and direct bilimbin in order to provide a reference range for the establishment of appropriate reference interval in this region.Methods From October to December in 2009,314 of physical examination population of Beijing Hospital were selected by questionnaire and laboratory test excluding liver and gallbladder diseases or metabolic diseases.Roche and Prodia reagents were used to test TBIL and DBIL,respectively.Reference interval was calculated by sex group and compared with the existing reference interval.Results The level of TBIL had a significant difference between different genders(P < 0.01).The level of TBIL had a significant difference between two kinds of reagents(P < 0.01).Using Roche reagent,the reference interval of TBIL was 7.1 ~ 27.2 μmol/L for man,and 4.8 ~ 20.9 μmol/L for woman,and the reference interval of DBIL was 1.4 ~6.8 μmol/L for man,and 0.9 ~5.7 μmol/L for woman.Using Prodia reagent,the reference interval of TBIL was 9.5 ~ 35.7 μmol/L for man,and 6.8 ~ 28.9 μmol/L for woman,and the reference interval of DBIL was 1.3 ~ 7.0 μmol/L for man,and 1.0 ~ 6.6 μmol/L for woman.Conclusions The level of TBIL and DBIL in physical examination population was higher than the existing reference interval.It is necessary to modify the existing reference interval and establish reasonable reference interval for different regions and gender,respectively.%目的 筛选健康参考个体,进行血清总胆红素和直接胆红素的检测,为建立适合本地区人群的血清胆红素参考区间提供参考.方法 2009年10月至12月本院体检人群通过调查问卷及实验室检查排除肝胆疾病及代谢性疾病,共选取314例.应用两个不同的检测体系进行血清总胆红素和直接胆红素的检测.以性别分组计算参考区间,并与现行参考区间进行比较.结果 不同性别间总胆红素水平差异有统计学意义(P<0.01),两

  12. Clinical observation of bilirubin adsorption combined with plasma exchange therapy in the treatment of hepa-topulmonary syndrome respiratory failure%胆红素吸附联合血浆置换治疗肝肺综合征呼吸衰竭的临床观察

    Institute of Scientific and Technical Information of China (English)

    杨春霞; 马小芳; 邓春艳

    2015-01-01

    目的:观察胆红素吸附联合血浆置换治疗肝肺综合征呼吸衰竭效果,探讨作用机制。方法134例各种原因肝病合并肝肺综合征患者随机分为血液净化组和对照组,测定纤支镜肺灌洗液中胆红素、IL-6浓度,监测两组机械通气( MV)参数变化,血气分析监测氧代谢情况,评估血液净化对肝肺综合征合并呼吸功能衰竭的治疗效果。结果与对照组相比,血液净化组纤支镜肺灌洗胆红素浓度、IL-6浓度( P<0.05),均明显下降,机械通气脱机时间早,时间短,呼吸机支持力度小(P<0.05),氧分压升高而肺泡-动脉氧分压差下降(P<0.05),肺功能明显改善。结论血液净化可有效治疗肝肺综合征改善肺功能,纠正呼吸衰竭,原因与清除肺组织局部胆红素沉积、炎症介质等因索有关。%Objective To observe the clinical effect of bilirubin adsorption combined with plasma exchange therapy in the treatment of hepatopulmonary syndrome respiratory failure. Methods 134 cases of various cause liver disease combined with hepatopulmonary syndrome patients were randomly divided into the blood purification group and the control group. The concentration of bilirubin and IL-6 in bronchoscopy lung lavage fluid was determined. Me-chanical ventilation ( MV) parameters, oxygen metabolism and blood gas analysis were monitored. Results Com-pared with the control group, the concentration of bilirubin and IL-6 decreased significantly in the blood purification group (P<0. 05). The blood purification group had earlier weaning time of mechanical ventilation, shorter ventila-tion time, and smaller ventilator support than the control group (P<0. 05). The improvement of pulmonary function was more pronounced in the blood purification group than in the control group. Conclusion Blood purification can effectively improve lung function and correct respiratory failure in the treatment of hepatopulmonary syndrome respira

  13. Estudo comparativo entre a medida plasmática e transcutânea de bilirrubina em recém-nascidos Factores asociados a la hipotermia durante transporte intrahospitalario en pacientes internados en una unidad de terapia intensiva neonatal Comparative study between plasma and transcutaneous bilirubin measurements in newborns

    Directory of Open Access Journals (Sweden)

    Patrícia Povaluk

    2011-03-01

    postnatal y presencia de factores de riesgo para hiperbilirrubinemia significativa. RESULTADOS: La asociación entre bilirrubina plasmática y transcutánea, en el momento de la indicación y después de 24 horas de fototerapia en las regiones frontal y esternal fue muy homogénea, debido a la fuerte correlación y los intervalos de confianza estrechos, tanto a 95% como a 99%. Se observó, además, respecto a la medida transcutánea en el área cubierta, 24 horas después del inicio de la fototerapia, la medida en el área esternal presentó correlación más fuerte con la plasmática (r=0,8599; p=0,0001. Las variables del RN analizadas no interfirieron significativamente en las medidas de bilirrubina. CONCLUSIÓN: Las dosificaciones transcutánea y plasmática presentan correlación fuerte antes de la fototerapia en las regiones frontal y esternal. Tras 24 horas de la fototerapia, la medida transcutánea esternal en área cubierta presentó mejor correlación.OBJECTIVE: To compare transcutaneous and plasma bilirubin measurements before and during phototerapy, on exposed and covered body areas, and to verify the association of the obtained levels with neonatal characteristics. METHODS: This cross-sectional study enrolled 44 newborn infants from April to October 2008. Simultaneous plasmatic and transcutaneous (frontal and sternal regions bilirubin assays were performed before and 24 hours after the beginning of phototerapy. On frontal and sternal regions, a small cover was placed and transcutaneous measurement was obtained from covered and exposed adjacent areas. The association between the measurements and neonatal weigh, sex, race, gestational and postnatal ages and risk factors for severe hyperbilirubinemia was calculated. RESULTS: There was a strong correlation between plasma and transcutaneous bilirubin assays measured in the frontal and sternal regions before the phototerapy, with narrow 95 and 99% confidence intervals. The covered sternal area presented the strongest

  14. Relationship between serum bilirubin, uric acid and cognition impairment in patients with subcortical ischemic vascular disease%皮质下缺血性血管病患者血清胆红素和尿酸水平与认知损害的关系

    Institute of Scientific and Technical Information of China (English)

    周霞; 王龙; 刘寒; 孙中武

    2014-01-01

    目的 探讨皮质下缺血性血管病(SIVD)患者血清胆红素和尿酸水平的变化及其与认知损害之间的关系.方法 将161例SIVD患者按认知损害程度分为皮质下血管性轻度认知损害组(SVMCI,n =79)及皮质下血管性痴呆组(SVaD,n=82),77名认知功能正常的老年人作为对照组.测定其血清直接胆红素(DBIL)、间接胆红素(IBIL)、总胆红素和尿酸水平;进行简易精神状态检查(MMSE)及剑桥老年认知检查表-中国修订版(CAMCOG-C)等神经心理学评价;依据头颅MRI表现对SIVD患者白质疏松程度进行分级.比较各组间血清胆红素和尿酸水平的变化,并将认知功能评分(MMSE评分)与胆红素、尿酸及血管危险因素进行多元回归分析.结果 SVaD和SVMCI患者MMSE评分(17.9±5.01、24.84±1.57)及CAMCOG-C评分(59.87±12.89、83.66±4.79)均低于对照组(28.19±1.03、91.66 ±4.93),差异均具有统计学意义(Z=197.63,P=0.000;Z=186.54,P=0.000).在校正性别、年龄及体重指数等因素后,SVaD患者的血清DBIL、IBIL和总胆红素水平均显著低于对照组;SVMCI组IBIL水平低于对照组,差异具有统计学意义.SVMCI和SVaD患者血清尿酸水平均显著高于对照组.对SIVD患者进行相关分析发现,胆红素与CAMCOG-C评分子项中语言及近记忆得分呈正相关(r=0.130,P=0.045;r =0.160,P=0.014);尿酸与MMSE、CAMCOG-C评分呈负相关(r=-0.180,P=0.005;r=-0.203,P=0.002).在控制性别、年龄、受教育程度、体重指数及高血压、糖尿病、吸烟等相关血管危险因素后,尿酸与MMSE之间仍存在一定的负性相关关系(β=-0.121,P=0.044),当进一步校正白质疏松分级后,尿酸与MMSE之间的关系并不显著.结论 SIVD患者血清胆红素水平显著降低,且与认知功能呈正相关;而尿酸水平显著增高,与认知功能呈负相关,尿酸对SIVD患者认知损害的影响与白质疏松程度密切相关.%Objective To detect the levels of serum bilirubin and uric

  15. 射血分数正常的心力衰竭患者血清胆红素水平与舒张功能不全的相关性研究%The relation study between serum bilirubin and diastolic cardiac dysfunction in heart failure with preserved ejection fraction patients

    Institute of Scientific and Technical Information of China (English)

    袁博; 任晖; 巩洁; 李静; 郑时康; 王甲文; 王蕊; 高天林

    2015-01-01

    目的:探讨射血分数正常的心力衰竭( HFpEF)患者血清胆红素水平与舒张功能不全的相关性。方法2014年1—12月诊治HFpEF患者80例为HFpEF组,根据超声检查结果分为3个亚组:轻度亚组13例、中度亚组44例、重度亚组23例,另选取健康体检者30例为健康对照组。通过心脏超声测量左房内径、左室舒张末期内径、左室短轴缩短率、左室射血分数、左室舒张末期容积、E峰、A峰、舒张早期二尖瓣环运动速度,并计算E/Ea值。综合统计分析胆红素水平与心脏超声各项结果的关系。结果中度亚组和重度亚组HFpEF患者总胆红素( TB)和直接胆红素(DB)均明显高于健康对照组(TB:t 中度=1?.732, P =0.045;t 重度=2.032, P =0.025。 DB:t 中度=2.732, P =0.007;t 重度=2.036, P =0.026),HFpEF患者组内比较可见,重度亚组HFpEF患者血清TB和DB明显低于轻度亚组(TB:t =1.715, P =0.047;DB:t =2.032, P =0.024)。 HFpEF组患者Ea峰明显低于健康对照组( t =0.010, P =0.017),而E/Ea高于健康对照组( t =0.022, P =0.032)。重度亚组患者Ea峰和E/Ea均明显差于轻度亚组(Ea峰:t =2.079, P =0.023;E/Ea:t =2.411, P =0.011)。 TB及DB水平与E/Ea呈负相关( r =-0.401, P =0.040;r =-0.434, P =0.030)。通过多元逐步回归分析表明,血清TB水平可作为自变量能分别进入E峰( P =0.003)和Ea峰( P =0.003)为因变量的线性回归模型方程。结论胆红素水平与心室舒张功能密切相关,可能是影响HFpEF发生机制中的重要危险因素。%Objective To investigate the correlation between serum bilirubin level and diastolic dysfunction in pa -tients with heart failure (HFpEF) with normal ejection fraction.Methods From 2014 January to 2014 December, 80

  16. 精神分裂症患者血清白蛋白、尿酸、胆红素和谷酰转肽酶水平的研究%A study on serum levels of albumins,uric acid,bilirubin and γ-glutamyl transpeptadase in schizophrenia patients

    Institute of Scientific and Technical Information of China (English)

    温盛霖; 程敏锋; 王厚亮; 岳计辉; 王宏; 钟志勇; 郑俩荣

    2012-01-01

    目的 探讨精神分裂症患者是否存在抗氧化能力降低及氧化应激损伤.方法 对精神分裂症住院患者208例,用BPRS评定疾病的严重程度,PANSS量表评估精神症状,成人韦氏智力量表评定智商;检测血清白蛋白、尿酸、胆红素和谷酰转肽酶的血浆水平.另选84名正常人作对照.结果 (1)患者组白蛋白、胆红素和谷酰转肽酶[(44.07±3.78)g/L,(11.57±6.84)μmol/L,(20.26±10.87)U/L]水平显著低于对照组(P0.05].(2)女性患者的血浆尿酸、胆红素和谷酰转肽酶[(293.80±92.63)μmol/L,(10.01±4.85)μmol/L,(17.68±8.36)U/L]水平均显著低于男性患者(P0. 05]. Serum levels of uric acid, bilirubin and γ-glutamyl transpeptadase were significantly tower in female patients than those in male patients[ (293. 80 ±92. 63) μmol/L, ( 10. 01 ±4. 85) μmol/L, ( 17. 68 ± 8. 36)U/L,P < 0. 05]. Serum levels of albumins, bilirubin and γ-glutamyl transpeptadase were significantly tower in female patients [ (42.92 ±4. 17) g/L, (10. 01 ±4. 85) μmol/L, (17. 68 ±8. 36) U/L] than those in female control (P < 0. 05) ,but serum uric acid levels [ ( 293. 80 ± 92. 63 ) μmol/L ] was significantly higher than those in female control. The negative symptom subscore and total score of BPRS were negatively correlated with serum levels of albumins (r = - 0. 267 , P = 0. 023 , r= - 0. 243 , P = 0. 043 ) . The VIQ and PIQ were positively correlated with serum of levels of 7-glutamyl transpeptadase ( r = 0. 388 , P = 0. 001; r = 0. 348, P = 0. 004 ). Conclusions Female patients with schizophrenia have abnormal free radical metabolism and oxidative stress. The tower glutamic acid and oxidative stress may be involved in the palhology of schizophrenia by means of effecl of cognitive function.

  17. 母乳性黄疸患儿血清总胆红素和胆汁酸测定的临床意义%Clinical Significance of Detecting the Total Bilirubin and Total Bile Acid of Breast-Feeding Jaundice Infants

    Institute of Scientific and Technical Information of China (English)

    付纳新; 徐建英; 刘俊芬

    2013-01-01

    Objective To explore clinical significance of detecting the total bilirubin (TBil) and total bile acid(TBA)of breast-feeding jaundice infants. Methods From January 2011 to April 2012,a total of 66 infants with breast-feeding jaundice were included in the study(research group,n = 66) ,meanwhile,30 health infants were also accepted as control group(n = 30). Ankle saphenous vein blood sampling(2 mL) was taken from each infants. Serum TBil,TBA and cholesterol (CHO) were detected respectively in two groups. The study protocol was approved by the Ethical Review Board of Investigation in Human Being of Baotou Third Affiliated Hospital. Informed consent was obtained from the parents of each participating neonate. Results Serum TBil,TBA and CHO of neonates were significant higher in research group than those of control group (P0.05)(本研究遵循的程序符合本院人体试验委员会所制定的伦理学标准,得到该委员会批准,分组征得受试对象监护人的知情同意,并与之签署临床研究知情同意书).结果 两组新生儿血清TBil,TBA和CHO水平比较,差异均有统计学意义(P<0.001).研究组新生儿血清TBil与TBA水平呈显著正相关关系(r=0.412,P<0.05).结论 母乳性黄疸新生儿可能自身存在胆汁淤积.

  18. 2型糖尿病下肢血管病变患者血清总胆红素水平及与血小板参数的关系%Relationship between serum total bilirubin level,diabetic nephropathy and platelet parameters in patients with T2DM accompanied with peripheral arterial disease

    Institute of Scientific and Technical Information of China (English)

    晏丕军; 冯健; 张志红; 徐勇; 欧阳芳; 何建华; 李佳; 马红艳

    2016-01-01

    目的:探讨2型糖尿病(T2DM)下肢血管病变(PAD)患者血清总胆红素(TBIL)水平与糖尿病肾病(DN)和血小板(PLT)参数的关系及其在PAD发病中的作用机制。方法145例 T2DM 患者,根据踝肱指数( ABI)分为下肢血管病变组( PAD 组,64例)和非下肢血管病变组( NPAD组,81例),采用酶法测定血清TBIL水平,并分析血清TBIL水平与ABI、胱抑素( Cys)-C、尿微量白蛋白/尿肌酐( UACR)、预估肾小球滤过率(eGFR)、PLT参数和血糖、血脂、血压等指标的关系。结果 PAD组血清 TBIL水平明显低于 NPAD 组〔(10.25±3.01)vs(12.68±3.51)μmol/L,P<0.01〕。相关分析显示PAD患者血清TBIL水平与直接胆红素( DBIL)、间接胆红素( IBIL)、血小板( PLT)计数、ABI和eGFR呈明显正相关( P<0.05或P<0.01),与PLT平均体积(MPV)、PLT比容(PCT)、血肌酐(Scr)、Cys-C和UACR呈明显负相关(P<0.05或 P<0.01)。多元逐步回归分析显示, ABI、Cys-C和MPV是影响PAD患者血清TBIL水平的独立相关因素。结论 PAD患者血清TBIL与DN、PLT参数有关,其可能通过增加氧化应激、炎症反应和胰岛素抵抗,引起血管内皮功能失调、血栓形成和动脉粥样硬化等,进而参与PAD的发生发展。%Objective To investigate the change of serum total bilirubin ( TBIL) level and evaluate the relationship of TBIL with di-abetic nephropathy (DN) and platelet parameters in patients with type 2 diabetes mellitus (T2DM) accompanied with peripheral arterial dis-ease(PAD),andexploretheroleandmechanismsofTBILinPADpatients.Methods 145T2DMpatientsweredividedintoPADgroup (64 cases) and NPAD group (81 cases) according to ankle brachial index (ABI).Fasting serum TBIL levels were measured by enzymatic method.The relationship between serum TBIL levels,ABI,cystatin(Cys)-C,urine microalbuminuria:creatinine ratio (UACR),estimated glo

  19. Determining Prevalence of Acute Bilirubin Encephalopathy in Developing Countries

    Science.gov (United States)

    2015-11-11

    Demonstrate BIND II Score of >=5, is Valid for Detecting Moderate to Severe ABE in Neonates <14 Days Old.; Demonstrate Community-BIND Instrument, a Modified BIND II, is a Valid and Reliable Tool for Detecting ABE.; Demonstrate That Community-BIND Can be Used for Acquiring Population-based Prevalence of ABE in the Community.

  20. 血清胆红素、糖化血红蛋白及血脂联合检验在2型糖尿病合并冠心病的临床作用分析%Clinical Effect of Serum Bilirubin,Glycosylated Hemoglobin and Blood Lipid Joint Inspection in Type 2 Diabetes Mellitus and Coronary Heart Disease

    Institute of Scientific and Technical Information of China (English)

    马亚平

    2016-01-01

    Objective To analyze serum bilirubin(TBIL),glycosylated hemoglobin(HbA1c)and blood fat joint inspection in type 2 diabetes melitus(T2DM) with coronary heart disease(CHD)in clinical effect.Methods From August 2013 to December 2015,140 T2DM patients of our hospital as research target,according to whether the combination of CHD were divided into group A(n=78, unincorporated CHD)and group B (n=62, with CHD). TBIL,HbA1c,blood lipids and other indicators for testing in 2 groups of patients, the test results were compared.ResultsCompared with A group, B group of HDL-C,TBIL decreased in patients,with LDL-C、HbA1c、TC、TG increased,the difference between the 2 groups had statistical significance(P<0.05). Conclusion TBIL,HbA1c,blood lipid joint detection were helpful for accurate diagnosis whether type 2 diabetes melitus with coronary heart disease,provide guidance for clinical diagnosis and treatment.%目的:分析血清胆红素(TBIL)、糖化血红蛋白(HbA1c)及血脂联合检验在2型糖尿病(T2DM)合并冠心病(CHD)的临床作用。方法选取2013年8月~2015年12月我院收治的140例T2DM患者作为观察目标,按照其是否合并CHD分为A组(n=78,未合并CHD)和B组(n=62,合并CHD),对2组患者TBIL、HbA1c、血脂等指标进行检测,比较检测结果的差异性。结果与A组患者相比,B组患者TBIL、HDL-C降低,LDL-C、HbA1c、TC、TG升高,2组间比较差异具有统计学意义(P<0.05)。结论 TBIL、HbA1c、血脂联合检测有利于准确诊断2型糖尿病是否合并冠心病,为临床选择针对性诊疗方法提供指导信息。

  1. Clinical Study of Serum Bilirubin in Patients with Coronary Heart Disease and Low Density Lipoprotein Cholesterol Level%冠心病患者血清胆红素和低密度脂蛋白胆固醇水平的临床研究

    Institute of Scientific and Technical Information of China (English)

    赖秀梅

    2013-01-01

    Objectives 35-55 year-old middle-aged men with coronary heart disease (CHD) patients and male healthy subjects, serum bilirubin (BIL) and low-density lipoprotein cholesterol (LDL-C) levels were retrospectively analyzed to explore the middle-aged menserum BIL and LDL-C levels and CHD. Methods Fuxin Second Hospital in January 2012 to November 2012 and confirmed 72 cases of CHD 35-55 year-old middle-aged male patients as the experimental group, 72 cases of the same age male healthy subjects served as controls group. Using automatic biochemical analyzer to detect various groups of subjects serum BIL and LDL-C level, were statistically analyzed. Results CHD patients serum TBIL and, DBIL IBIL concentration were lower than the control group, LDL-C concentration higher than that in the control group, a statistically significant difference (P<0.05). Serum TBIL, the DBIL and, IBIL level of LDL-C concentration were negatively correlated (-1

  2. Combined CVVH-bilirubin adsorption therapy on patients with hyperbilirubinemia%组合式连续性静脉-静脉血液滤过-胆红素吸附系统在高胆红素血症治疗中的应用

    Institute of Scientific and Technical Information of China (English)

    朱冬冬; 龚德华; 徐斌; 刘志红; 季大玺

    2011-01-01

    血酶原时间(PT),国际标准化比值(INR)均较治疗前延长。组合法单次治疗后血尿素氮(BUN),血清肌酐(SCr),谷丙转氨酶(ALT),谷草转氨酶(AST)均有下降,对TP,ALB,凝血功能无影响。组合法治疗后患者收缩压、心率,临床APACHEII有所改善,MELD评分改善显著。患者ICU 30d存活率达69%,出院存活率56%。两种方法治疗过程中患者生命体征平稳,无不良事件发生。结论:组合式CVVH-胆红素吸附疗法是一种新型有效的人工肝支持治疗。可显著降低患者胆红素水平,改善生化检查指标及部分临床症状,且患者耐受性好。相较血浆分离灌流方法(常规法),其对患者的白蛋白及凝血功能无影响,且可改善患者APACHE Ⅱ及肝功能MELD评分,是一种值得在临床推广应用的人工肝支持疗法。%Objective: To observe the clinical efficacy and safety of combined CVVH-bilirubin adsorption treatment on patients with hyperbilirubinemia and compare to the traditional plasma separation perfusion. Methodology; Sixteen cases with hyperbilirubinetnia received total 55 sessions artificial liver support therapy ( ALS).All of them weretreated with conventional plasma perfusion (conventional method) for the first treatment. The rate of blood flow was set at 100 ~ 120 ml/min and the rate of plasma separated was set at 30 ~ 40 ml/min. Then they were treated with combined CVVH-bilirubin adsorption (combination method). The rate of blood flow was set at 200 ~ 250 ml/min and the rate of plasma separated was set at 90 ml/min. Separated blood plasma components were filtrated by the CVVH filter AV600 when ultrafiltration rate was set at 66 ml/min. Then concentrated plasma components were adsorpted by BRS-350 adsorption column and they were reinfusioned in vivo. At the same time bicarbonate replacement fluid at the speed of 4 000 ml/hr with pre-dilution mode was input before plasma component separator EC40W. ALS was

  3. Ionization of tyrosine residues in human serum albumin and in its complexes with bilirubin and laurate

    DEFF Research Database (Denmark)

    Honoré, B; Brodersen, R

    1992-01-01

    Spectrophotometric titration of human serum albumin indicates that ionization of the 18 tyrosine residues takes place between pH 9 and 12.7. A Hill plot indicates that protons dissociate co-operatively from tyrosine residues, in pure albumin between pH 11.0 and 11.4 with a Hill coefficient 1.7, a...

  4. IMMUNOAFFINITY PURIFICATION AND RECONSTITUTION OF THE HUMAN BILIRUBIN PHENOL UDP-GLUCURONOSYLTRANSFERASE FAMILY

    NARCIS (Netherlands)

    SEPPEN, J; JANSEN, PLM; ELFERINK, RPJO

    1995-01-01

    When membrane proteins are solubilized and subjected to purification procedures, the loss of lipids surrounding the protein often results in irreversible inactivation. We describe a procedure for the immunoaffinity purification of the membrane protein UDP-glucuronosyltransferase from human liver. Th

  5. Clinical Significance of Serum Bilirubin Detection of Patient with Coronary Heart Disease

    Institute of Scientific and Technical Information of China (English)

    TAOLi; LUORui; ZHUANGDiankui

    2004-01-01

    To explore the relation between serum bilinabin and eoronary heart disease Methods Compare the level of serum bilinabin among patients with eoronary heart disease, patients with other disease and normal persons. Results The level of serum bilinabin of patients with coronary heart disease is higher than that of normal persons. Conclusion The reduction of density of serum bihrubin is one of the independent risk factors of coronary heart disease.

  6. 血清总胆红素水平与ST段抬高型心肌梗死患者经皮冠状动脉介入治疗术前梗死相关动脉血流及院内主要心脏不良事件的相关性研究%Correlations Between Serum Total Bilirubin Level and Blood Flow of Infarction Related Artery Before PCI,and Major Adverse Cardiac Events During Hospitalization in Patients With ST-segment Elevation Myocardial Infarction

    Institute of Scientific and Technical Information of China (English)

    王树源; 黄家卓

    2016-01-01

    ,respectively(P < 0. 05). Conclusion Serum total bilirubin level has some correlations with blood flow of infarction related artery before pCI,and with major adverse cardiac events during hospitalization in patients with ST - segment elevation myocardial infarction.

  7. Study on the-Redox Process of Bilirubin and Biliverdin at Platinum Electrode by in Situ Spectroelectrochemistry

    Institute of Scientific and Technical Information of China (English)

    牛建军; 董绍俊

    1994-01-01

    The electrochemical redox behavior of bilirubin(BR Ⅳα),biliverdin(BV Ⅳα)and theiroxidized product bile-purpurin(Bi-Pu)have been studied by in situ spectroelectrochemical techniques,whichreveals that the transformation of BR Ⅳα■BV Ⅳα■Bi-Pu can be achieved by controlling poten-tials.The kinetic investigation has shown that the heterogeneous electron transfer reactions of the couples ofⅠ/Ⅱ and Ⅳ/Ⅲ were quasi-reversible and irreversible at a clean platinum electrode with the formal heteroge-neous electron transfer rate constants 1.5×10-4cm·s-1and 4.8×10-5cm·s-1,respectively.

  8. Interplay of co-inherited diseases can turn benign syndromes in a deadly combination : haemoglobinopathy and bilirubin transport disorder

    NARCIS (Netherlands)

    Stolmeijer, T. M.; van den Berg, A. P.; Koeze, J.; Gouw, A. S. H.; Croles, F. N.; Sieders, E.; Zijlstra, J. G.

    2015-01-01

    We present a case about a 25-year-old male patient suffering from a rare genetic disorder called Mizuho haemoglobin. He was admitted to the Intensive Care Unit with acute liver and renal failure. During admission he also developed a cardiac tamponade twice. Finally he received a liver transplantatio

  9. Coupled plasma filtration adsorption reduces serum bilirubine in a case of acute hypoxic hepatitis secondary to cardiogenic shock.

    Science.gov (United States)

    Caroleo, Santo; Rubino, Antonino S; Tropea, Francesco; Bruno, Orlando; Vuoto, Domenico; Amantea, Bruno; Renzulli, Attilio

    2010-10-01

    Hypoxic hepatitis (HH) is a severe complication of postoperative low output syndrome, associated with high mortality rates despite appropriate drug therapy. Recently several extracorporeal supportive techniques have become available. We describe the case of a 70-year-old woman who developed HH secondary to cardiogenic shock after cardiac surgery. CPFA proved to be a valid tool for concomitant hemodynamic support and organ replacement therapy.

  10. Increased expression of low-density lipoprotein receptors in a Smith-Lemli-Opitz infant with elevated bilirubin levels.

    Science.gov (United States)

    Ness, G C; Lopez, D; Borrego, O; Gilbert-Barness, E

    1997-01-31

    We report on an infant girl with severe RSH or Smith-Lemli-Opitz syndrome with hyperbilirubinemia. The infant died at age 2 months. Sterol analysis of liver and brain tissues showed marked elevations of 7-dehydrocholesterol with decreased levels of cholesterol. Immunocytochemical analysis demonstrated remarkable increases in low-density lipoprotein (LDL) receptors in these tissues, indicative of a deficiency in available cholesterol for tissue needs.

  11. Follow-up of neonatal non-haemolytic hyperbilirubinemia in Danish term and near-term infants with total serum bilirubin level ≥ 420 umol/l

    DEFF Research Database (Denmark)

    Vandborg, Pernille Kure; Greisen, G; Hansen, B M;

    2010-01-01

    Although a light to moderate alcohol intake is associated with a lower risk of acute coronary syndrome (ACS), alcohol is also associated with risk of hypertension, which in turn is a strong risk factor of ACS. We examined whether middle-aged men and women with hypertension also benefit from a lig...

  12. Voltammetry and single-molecule in situ scanning tunneling microscopy of laccases and bilirubin oxidase in electrocatalytic dioxygen reduction on Au(111) single-crystal electrodes

    DEFF Research Database (Denmark)

    Climent, Victor; Zhang, Jingdong; Friis, Esben Peter;

    2012-01-01

    Laccases (E.C. 1.10.3.2) are multicopper oxidases catalytically active in the oxidation of diphenolics and related compounds by molecular dioxygen. The laccases contain a single-copper type I center and a trinuclear cluster of a single-copper type II and a dinuclear type III center. The oxidation...

  13. Design of experiments and principal component analysis as approaches for enhancing performance of gas-diffusional air-breathing bilirubin oxidase cathode

    Science.gov (United States)

    Babanova, Sofia; Artyushkova, Kateryna; Ulyanova, Yevgenia; Singhal, Sameer; Atanassov, Plamen

    2014-01-01

    Two statistical methods, design of experiments (DOE) and principal component analysis (PCA) are employed to investigate and improve performance of air-breathing gas-diffusional enzymatic electrodes. DOE is utilized as a tool for systematic organization and evaluation of various factors affecting the performance of the composite system. Based on the results from the DOE, an improved cathode is constructed. The current density generated utilizing the improved cathode (755 ± 39 μA cm-2 at 0.3 V vs. Ag/AgCl) is 2-5 times higher than the highest current density previously achieved. Three major factors contributing to the cathode performance are identified: the amount of enzyme, the volume of phosphate buffer used to immobilize the enzyme, and the thickness of the gas-diffusion layer (GDL). PCA is applied as an independent confirmation tool to support conclusions made by DOE and to visualize the contribution of factors in individual cathode configurations.

  14. THE ANATAGONISM OF BILIRUBIN ON DNA DAMAGE OF ICR MICE DUE TO TCE%胆红素对TCE致ICR小鼠DNA损伤的拮抗作用

    Institute of Scientific and Technical Information of China (English)

    涂程; 邰昌松; 杨青; 黎燕; 王绵珍

    2005-01-01

    目的:研究胆红素对三氯乙烯(TCE)致ICR小鼠肝、肾、外周血DNA损伤的影响.方法:应用单细胞凝胶电泳(SCGE)技术,检测TCE对ICR小鼠肝、肾、外周血细胞DNA损伤作用,同时观察20~200 μmol/L胆红素对TCE所致DNA损伤的保护作用.结果:TCE染毒组肝、肾、血细胞彗星率较对照组增加;胆红素各剂量组SCGE各指标在20~200μmol/L范围内先减小后增大,100μmol/L处最小.结论:100μmol/L左右的胆红素能较好的拮抗TCE对小鼠肾脏细胞DNA损伤.

  15. Diagnosis and Treatment of Unconjugated Hyperbilirubinemia

    NARCIS (Netherlands)

    Schreuder, Andrea Bertilde

    2014-01-01

    Bilirubine is een geel afbraakproduct van heem met tegenstrijdige eigenschappen. In lage concentraties is (ongeconjugeerd) bilirubine een antioxidant, maar in hoge concentraties is het zeer schadelijk voor onze hersencellen. Ophoping van (ongeconjugeerd) bilirubine in ons lichaam kan leiden tot perm

  16. Application of Brainstem Auditor Evoked Potential for Evaluating Bilirubin Nerval Toxicity in Preterm Infants%应用脑干听觉诱发电位监测早产儿胆红素神经损害的意义

    Institute of Scientific and Technical Information of China (English)

    吕回; 罗慧; 赵宁; 黄晓虹; 李耘; 赖剑蒲; 陆玲

    2005-01-01

    目的:探讨早产儿血清胆红素(SB)增高时导致神经系统损害时其SB临界值,以及影响临界值的高危因素.方法:收集2000-01~2002-12在我院新生儿科住院并出现黄疸的早产儿共167例,以脑干听觉诱发电位(BAEP)出现异常作为神经损害标志.入院后即检测SB浓度及BAEP,当出现皮肤黄疸后每日检测SB浓度及BAEP 1次,直至BAEP出现异常,并记录出现异常时对应的SB值.同时记录各患儿的胎龄、出生体重、血清白蛋白值和血pH值.结果:本组早产儿引起BAEP异常时,血清间接胆红素(SIB)平均临界值为(177.41±27.23)μmol/L.而出生胎龄越小,其临界值越低,出生胎龄<32周、~34周、~36+6周组的早产儿,其导致BAEP异常时,SIB平均临界值分别为(154.67±28.92)μmol/L、(161.61±16.06)μmol/L和(173.76±19.22)μmol/L.相关分析研究提示出生胎龄、出生体重、血清白蛋白浓度及血pH值均分别与SIB临界值有相关性,P值均<0.001.结论:早产儿导致神经损害的SB水平明显低于传统的病理性黄疸水平.在判断影响SB对中枢神经细胞毒性的高危因素时,应重视出生体重、血清白蛋白浓度及血pH值水平.

  17. Study on change of aminoacid content in the brain of newborn rabbits with bilirubin encephalopathy%肌兴奋仪在脑瘫儿童SPR术后康复中的应用

    Institute of Scientific and Technical Information of China (English)

    曹旭; 易斌

    2001-01-01

    目的观察肌兴奋仪对脑瘫儿童SPR术后肌力提高,功能改善的影响. 方法对我院收治的27例患者SPR术后进行肌兴奋仪治疗,同时选择36例患者作为对照.对两组结果进行统计分析.结果肌兴奋仪治疗组患者的术后肌力以及运动功能均高于对照组(P<0.05).结论肌兴奋仪能较快提高脑瘫儿童SPR术后的肌力,促进运动功能的改善.

  18. 几种主要的先天性胆红素代谢障碍性肝病的临床及病理研究%Clinical and pathological features of patients with hereditary disorders of bilirubin metabolism

    Institute of Scientific and Technical Information of China (English)

    孙艳玲; 赵景民; 辛绍杰; 李文淑; 周光德; 潘登; 刘树红

    2008-01-01

    目的 回顾性研究并分析几种主要的先天性胆红素代谢障碍性肝病的临床及病理学特点.方法 收集我院2000年以来7036例患者的活体肝组织穿刺病理学诊断(肝穿)资料,其中主要的几种先天性胆红素代谢障碍性肝病病例155例,包括Gilbert综合征115例,Dubin-Johnson综合征33例.Crigler-Najjar综合征5例,Roter综合征2例,并结合其临床资料、生化检查进行统计学比较分析;采用常规HE染色及胆色素、铁、铜、网状纤维和胶原纤维等特殊染色,结合部分免疫组织化学染色.探讨其临床及病理学特点.结果 该组155例先天性胆红素代谢障碍性肝病病例占总肝穿病例的2.2%,男女比例为5∶1,年龄2~49岁;病史1个月~39年;临床主要以眼黄、尿黄和皮肤黄染为主(60.0%),其次为乏力和纳差(20.6%)、脾大(21.9%)及肝大(17.4%)、胆红素升高(16.1%).实验室比较分析,Gilbert综合征和Crigler-Najjar综合征以间接胆红素(IBIL)增高为主,Dubin-Johnson综合征和Roter综合征以直接胆红素(DBIL)增高为主,其中有3例Dubin-Johnson综合征患者在发病过程中动态监测始终以IBIL增高为主;主要病理改变为:肝组织内小叶结构均基本完整,中央区周围/区域肝细胞浆内在Gilbert综合征和Crigler-Najjar综合征可见较细的棕褐色色素颗粒沉积,在Dubin-Johnson综合征可见较粗糙的棕黑色色素颗粒沉积,而Roter综合征几乎难觅色素颗粒;部分肝细胞轻度水样变性或脂肪变性,Kupffer细胞内无吞噬色素颗粒现象;小叶间胆管轻度增生,无纤维组织增生及界面炎改变.结论 先天性胆红素代谢障碍性肝病病例以男性为主,发病年龄以青少年居多,临床表现及实验室检杏具有一定特点,病史从几个月到十几年不等,未见慢性化病理改变,组织检查均显示其特殊的病理特征,但诊断与鉴别诊断中病理诊断仍为金标准.

  19. HPLC determination of bilirubin in paracetamol, caffein, atificial cow -bezoar and chlorphenamine maleate tablets%高效液相色谱法测定氨咖黄敏片中胆红素的含量

    Institute of Scientific and Technical Information of China (English)

    梁云霞; 李杰滨; 谭秀敏; 谭博; 赵悦辉

    2007-01-01

    目的:采用高效液相色谱法对氨咖黄敏片中胆红素进行含量测定.方法:选用Agilent C18分析柱,甲醇-氯仿-1%磷酸溶液(80:12:8)为流动相,检测波长为449 nm,流速1.0 ml/min,柱温30 ℃.结果:胆红素的线性范围为0.008~0.083 μg(r=1.000),平均回收率100.2%.结论:方法准确,回收率好.

  20. Protective effect of bilirubin and natural cow - bezoar on styrene - induced HepG2 cell DNA strand breaks%胆红素及牛黄拮抗苯乙烯所致肝癌细胞株损伤

    Institute of Scientific and Technical Information of China (English)

    魏雪涛; 蒋建军; 尚兰琴; 沈惠麒

    2004-01-01

    目的观察苯乙烯诱导的人肝癌细胞株(HepG2)细胞DNA链断裂损伤以及天然牛黄和胆红素对其损伤的拮抗效应.方法采用单细胞凝胶电泳法观察HepG2细胞体外暴露于苯乙烯出现的DNA链断裂损伤,同时观察天然牛黄和胆红素对于苯乙烯诱导DNA损伤的拮抗作用.结果在细胞存活未受到苯乙烯影响的浓度下,0.2μmol/L的苯乙烯即可诱导HepG2细胞明显的DNA链断裂损伤,彗星细胞频率和DNA迁移距离分别为(9.5±2.1)%和(4.5±0.7)μm.在明显诱导DNA损伤浓度的苯乙烯处理时同时加入天然牛黄或胆红素,发现二者对苯乙烯诱导的DNA链断裂损伤在一定浓度下均有良好的拮抗作用,10 μmol/L的胆红素或天然牛黄与1μmol/L的苯乙烯共同处理时,HepG2细胞的DNA损伤水平基本回复到本底水平.结论苯乙烯能够诱导HepG2细胞出现DNA链断裂损伤,10 μmol/L的胆红素或天然牛黄基本能完全拮抗苯乙烯诱导的DNA链断裂损伤.

  1. Determination of bilirubin in paracetamol,caffein, atificial cow-bezoar and chlorphenamine maleate capsules by RP-HPLC%氨咖黄敏胶囊中胆红素的含量测定

    Institute of Scientific and Technical Information of China (English)

    刘雁鸣; 郑金凤; 杨汉初

    2005-01-01

    目的采用反相高效液相色谱法测定氨咖黄敏胶囊中胆红素的含量.方法采用DiamonsilTMC18色谱柱(4.6 mm×200 mm,5μm),以甲醇-氯仿-1%磷酸溶液(85:13:2)为流动相,流速1.0 mL·min-1,检测波长450nm.结果胆红素在3.97~79.5μg·mL-1峰面积与浓度呈良好的线性关系(r=0.997),平均回收率为99.95%,RSD为0.38%(n=9).结论方法简便、精密度高、重现性好、结果准确可靠.

  2. A Cholesterol-Bilirubinate Gallstone Induced by Fasciola hepatica Infection in Macaca mulatta%猕猴自然感染肝片吸虫诱发胆管结石的形成

    Institute of Scientific and Technical Information of China (English)

    廖国阳; 卢明义; 谢晋

    2000-01-01

    在研究肝片吸虫诱发猕猴自发性胆结石形成的病理学基础上,采用红外光谱分析、原子吸收光谱分析和组织化学染色对胆结石的成分及结构进行了测定,初步探讨了本病发生的机理.在一只9岁雌性猕猴肝总胆管内发现4条肝片吸虫(Fasciola hepatica),胆囊胆汁中检出大量肝片吸虫虫卵.左侧胆管内有一颗棕黑色结石,直径为1 cm、长约2.5 cm圆柱形.肝细胞灶性坏死伴有轻度结缔组织增生,胆管腺体重度增生,上皮细胞胞质内含有中性与酸性混合型粘多糖物质,上皮间有大量杯状细胞.胆石切面呈环状,环层间含有粘多糖物质.胆石经红外光谱分析出现胆固醇-胆色素混合型胆石和黑色物质特征性吸收峰;原子吸收光谱分析钙元素含量最高.

  3. Effect of Urine Bilirubin on the Results of Urobilinogen Tested With Urine Dry Chemistry Analyzer%尿胆红素对干化学法检测尿胆原的影响

    Institute of Scientific and Technical Information of China (English)

    项贵明; 李阳; 蒲晓允

    2007-01-01

    目的 观察尿中胆红素对尿干化学分析仪检测尿胆原的影响.方法 对135例尿干化学分析仪检测尿胆红素和尿胆原均为阳性的标本和17例尿干化学分析仪检测尿胆红素阴性而尿胆原为阳性的标本,以及60例尿干化学分析仪检测尿胆红素和尿胆原均为阴性的标本,进行手工检测方法对比.结果 尿干化学分析仪在检测尿中存在胆红素时可使尿胆原出现假阳性结果.结论 当尿干化学分析仪检测尿胆红素和尿胆原均为阳性且尿胆红素在"++"以上的标本应通过其它检测来确定检验结果.

  4. 用CLSI EP方案评价总胆红素试剂分析性能%Evaluation of the analytical performance of total bilirubin(TBil) reagents with CLSI method evaluation guidline

    Institute of Scientific and Technical Information of China (English)

    刘冰

    2009-01-01

    目的 通过应用CLSI EP方案分别对国产和进口总胆红素(TBil)试剂的分析性能评价,了解国产总胆红素试剂分析性能能否满足临床需要.方法 在同一台全自动生化分析仪上,同时测定试剂准确度、精密度、最低检测限、线性范围、干扰试验、方法比对实验、稳定性观察等7项技术指标.结果 两种试剂准确度测定,其相对偏差≤±10%;批间变异系数<4.0%;国产TBil试剂线性范围0.5~865.0μmol/L,进口试剂线性范围0.4~869.0μmol/L;国产TBil试剂的最低检测限为0.5μmol/L,进口试剂的最低检测限为0.4μmol/L;方法比对实验结果Y=1.0069X+0.9366,R2=0.9993;抗干扰能力无明显差异;试剂稳定性相近.结论 国产总胆红素试剂与进口试剂的分析性能差异无统计学意义,能满足临床实验室的需要.

  5. Evaluation of the analytical performance of Direct Bilirubin (DBil) reagents according CLSI EP document%参照CLSI EP方案评价直接胆红素试剂分析性能

    Institute of Scientific and Technical Information of China (English)

    谢文辉

    2010-01-01

    目的 通过应用CLSI EP方案分别对国产和进口直接胆红素(DBil)试剂分析性能进行评价,了解国产直接胆红素试剂分析性能能否满足临床需要.方法 在同一台全自动生化分析仪上,同时测定试剂准确度、精密度、最低检测限、线性范围、干扰试验、方法比对实验、稳定性观察等7项技术指标.结果 两种试剂准确度测定,其相对偏差≤±10%;批间变异系数小于4.0%;国产DBil试剂线性范围0.2-595.0umol/L,进口试剂线性范围-0.4-595.7umol/L;国产DBil试剂的最低检测限为0.4umol/L,进口试剂的最低检测限为0.9umol/L;方法比对实验结果Y=0.997X+5.2467,R2=0.9996(浓度范围0.2-595.0umol/L);抗干扰能力无明显差异;试剂稳定性相近.结论 :国产直接胆红素试剂与进口试剂的分析性能无显著性差异,能满足临床实验室的需要.

  6. Therapeutic effect of turquoise versus blue light with equal irradiance in preterm infants with jaundice

    DEFF Research Database (Denmark)

    Ebbesen, Finn; Madsen, Poul; Støvring, Søren

    2007-01-01

    (OSRAM L18W/860 fluorescent lamps) or blue light (Philips TL20W/52 fluorescent lamps). The concentrations of serum total bilirubin and bilirubin isomers were measured by the Vitros routine method and by HPLC, respectively. RESULTS: The decrease in serum concentrations of total bilirubin, total bilirubin...... irradiance, expressed both by serum total bilirubin, total bilirubin isomers and Z,Z-bilirubin, i.e. the turquoise spectral range is more efficient than the blue. This is in accordance with deeper penetration into the skin, lower production of the Z,E-bilirubin and greater production of E......AIM: To compare the efficiency of turquoise light with that of TL52 blue in treatment of preterm infants with jaundice at the same level of body irradiance. METHODS: Infants with gestational age 28-37 weeks and non-haemolytic hyperbilirubinemia were treated for 24 h with either turquoise light...

  7. Napoved razvoja hiperbilirubinemije pri donošenih novorojenčkih z neinvazivnimi metodami: Prediction of hyperbilirubinemia by noninvasive methods in full-term newborns:

    OpenAIRE

    Bratanič, Borut; Pavlin, Tatjana; Gradecki, Mirjam; Furlan, Danijela; Žalec, Lidija; Oštir Mevželj, Darinka

    2013-01-01

    Introduction: The noninvasive screening methods for bilirubin determination were studied prospectively in a group of full-term healthy newborns with the aim of early prediction of pathological neonatal hyperbilirubinemia. Laboratory determination of bilirubin (Jendrassik- Grof (JG)) was compared to the noninvasive transcutaneous bilirubin (TcBIL) together with the determination of bilirubin in cord blood. Methods: The study group consisted of 284 full-term healthy consecutively born infants i...

  8. Changes in erythrocytic deformability and plasma viscosity in neonatal ictericia.

    Science.gov (United States)

    Bonillo-Perales, A; Muñoz-Hoyos, A; Martínez-Morales, A; Molina-Carballo, A; Uberos-Fernández, J; Puertas-Prieto, A

    1999-01-01

    We studied 45 full-term newborns divided into 3 groups. Group 1: 17 newborns with bilirubin ictericia (bilirubin 11-20 mg/dL) and Group 3: 10 newborns with moderate hemolytic ictericia needing exchange transfusion. The following were studied: erythrocytic deformability, plasma viscosity, plasmatic osmolarity, seric bilirubin, bilirubin/albumin ratio, free fatty acids and corpuscular volume of the erythrocytes. In full-term newborns, the following are risk factors for increased erythrocytic rigidity: neonatal hemolytic illness (p = 0.004, odds ratio: 7.02), increases in total bilirubin (p = 0.02, odds ratio: 4.3) and increases in the bilirubin/albumin ratio (p = 0.025, odds ratio: 4.25). Furthermore, the most important risk factor for high plasma viscosity is also neonatal hemolytic illness (p = 0.01, odds ratio: 2.30). The role of total bilirubin is also important (p = 0.09, odds ratio: 2.10), while that of the bilirubin/albumin ratio (p = 0.012, NS) is less so. The greater the hemolysis, the greater the erythrocytic rigidity and plasma viscosity (p ictericia, hemolytic illness and increases in the bilirubin/albumin ratio are accompanied by rheological alterations that could affect cerebral microcirculation and cause a neurological deficit not exclusively related to the levels of bilirubin in plasma.

  9. Mechanism involved in the UCB neurotoxicity on cellular models

    OpenAIRE

    Giraudi, Pablo Jose'

    2009-01-01

    Summary This doctoral thesis covers three years period (2006-2008) during which I have investigated the bilirubin neurotoxicity in the neuroblastoma SH-SY5Y cell line, a neuronal cell model widely used in the study of the pathogenesis and in the development of new therapeutic compounds for neurodegenerative diseases. In the first chapter is summarized the current knowledge about bilirubin chemistry and metabolism including disorders of bilirubin metabolism and the neuronal disturbanc...

  10. Investigation of Surface-Enhanced Raman Spectra of Human Gallstones on Colloidal Silver

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    The surface-enhanced Raman scatterihg (SERS) spectra of human gallstones is investigated. Complicated Raman bands were due to multiple components that include bilirubin, bilirubinate salts, cholesterol, fatty salts and proteins. The results suggest that besides bilirubin and cholesterol, fatty salts and proteins may play an important role in the formation of gallstone. The experimental data supply an useful information for the research of the formation mechanism of gallstones.

  11. Disease: H00208 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available H00208 Hyperbilirubinemia; Crigler-Najjar syndrome, type I (CN1); Crigler-Najjar sy...dromes result in unconjugated hyperbilirubinemia caused by deficiency of bilirubin-UDP-glucuronosyltransfera...se which is involved in the detoxification of bilirubin by conjugation with glucu...ronic acid. Gilbert disease is a benign familial disorder characterized by low-grade chronic hyperbilirubine...sta E Hematologically important mutations: bilirubin UDP-glucuronosyltransferase gene mutations in Gilbert a

  12. Antioxidant status in neonatal jaundice before and after phototherapy

    OpenAIRE

    Ayyappan, S.; Sachu Philip; N Bharathy; V. Ramesh; Naveen Kumar, C.; Swathi, S.; A.Arun Kumar

    2015-01-01

    Background: Neonatal jaundice refers to yellow coloration of the skin and the sclera (whites of the eyes) of newborn babies that result from the accumulation of bilirubin in the skin and mucous membranes. Because bilirubin is potentially toxic to the central nervous system. Genetic disorders of bilirubin conjugation, particularly the common Gilbert's syndrome, can also contribute to neonatal hyperbilirubinemia. Objective: The aim of this study was to evaluate the lipid per-oxidation and antio...

  13. Studies on the Mechanism of Fasting Hyperbilirubinemia

    Science.gov (United States)

    bilirubin from the plasma (CBr) was reduced by 28 to 54% during fasting , accounting for the increase in Br. The ratio of the plasma bilirubin...turnover during fasting to that in base line state was 0.99 plus or minus 0.16 (mean plus or minus SD) for these five individuals, indicating that increased...plasma bilirubin turnover did not contribute to the rise in Br. In 5 additional subjects the mean change in carbon monoxide production with fasting

  14. The correlation between anemic parameters and bilirubin level of the patients and the disease activity of systemic lupus erythematosus%系统性红斑狼疮患者贫血参数及胆红素水平与疾病活动关系的研究

    Institute of Scientific and Technical Information of China (English)

    肖平; 刘玲玲

    2006-01-01

    系统性红斑狼疮(SLE)是一种自身免疫性结缔组织病,由于体内有大量致病性自身抗体和免疫复合物,造成组织损伤.临床上出现多个系统和脏器的损害。当前对诊断SLE患者疾病活动的指标大多为补体,抗dsDNA抗体,ENA多肽抗体。近来SLE贫血患者的血红蛋白(Hb)、网织红细胞参数(RetI)及血清胆红素(BIL)水平的变化报道较少。本次研究探讨SLE贫血患者的Hb、RetI、BIL水平的变化及与病情发展的关系.现报道如下。

  15. Influence of tertiary prevention rehabilitation nursing on behavioral development of patients with neonatal bilirubin encephalopathy%三级预防康复护理对新生儿胆红素脑病患儿行为发育的影响

    Institute of Scientific and Technical Information of China (English)

    梁贡南

    2011-01-01

    [目的]观察三级预防康复护理对新生儿胆红素脑病患儿行为发育的影响效果.[方法]将64例胆红素脑病患儿按时间段分成观察组和对照组,对照组给予传统的常规治疗与护理,观察组在对照组的基础上实施三级预防康复护理.两组患儿于6个月、1岁、2岁时进行盖塞尔(Gesell)行为发育测试.[结果]观察组患儿在6个月、1岁、2岁时Gesell行为发育测试时发育商数(DQ)较对照组高,差异有统计学意义(P<0.05).[结论]三级预防康复护理对促进新生儿胆红素脑病患儿的行为发育有显著效果.

  16. 牛黄及胆红素对三氯乙烯染毒小鼠脂质过氧化的拮抗作用%The antagonism of cow-bezoar and bilirubin on the lipid peroxidation of mice induced by trichloroethylene

    Institute of Scientific and Technical Information of China (English)

    邰昌松; 赵萍; 黎燕; 杨青; 沈惠麒

    2005-01-01

    目的研究牛黄、胆红素对三氯乙烯(TCE)染毒ICR小鼠所致的脂质过氧化的拮抗作用.方法用TCE灌胃染毒ICR小鼠制造脂质过氧化模型,然后分别以牛黄、胆红素灌胃,测定ICR小鼠肝、肾组织中丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、过氧化氢酶(CAT).结果与阴性对照组比,TCE处理组肝、肾组织GSH-Px、 SOD、CAT活力降低,MDA含量增加,差异均有显著性(P<0.05);牛黄、胆红素染毒与TCE处理组比较,肝、肾组织GSH-Px、 SOD、CAT 活力显著增强(P<0.05),脂质过氧化产物MDA含量减少.结论牛黄和胆红素均能较好地拮抗TCE所引起的ICR小鼠脂质过氧化.

  17. 肝细胞膜钙泵与钠泵活性变化在胆红素钙结石形成中的作用%The Effects of Activities of Ca2+-ATPase and Na+-K+-ATPase in Plasma Membranes of Hepatocytes on the Formation of Calcium Bilirubinate Gallstone

    Institute of Scientific and Technical Information of China (English)

    庄文; 肖路加; 左凤琼; 吴兆锋; 吴忠舜

    2002-01-01

    目的探讨肝细胞膜钙泵和钠泵活性变化在胆红素钙结石形成中的作用.方法建立胆红素钙结石兔模型,对照组28只,胆道不全梗阻组(BO)36只,胆道不全梗阻加感染组(BOI)39只,各组再按术后处死时间(3、7、14和20天)分组,动态测定各组各时相肝细胞膜钙泵、钠泵活性及肝细胞钙含量.结果 BOI组及BO组肝细胞钙进行性增多,而肝细胞膜钙泵、钠泵活性呈进行性下降,与对照组相比差异有显著性(P<0.01),BOI组上述变化较BO组明显(P<0.05).结论兔胆红素钙结石形成过程中存在肝细胞膜钙泵和钠泵活性渐进性下降.钙泵、钠泵活性下降引起肝细胞钙超负荷,从而促进成石.

  18. 肝细胞膜钠泵活性变化在胆红素钙结石成石过程中的作用%Effects of Activities of Na+-K+-ATPase in Plasma Membranes of Hepatocytes in the Formation of Calcium Bilirubinate Gallstone

    Institute of Scientific and Technical Information of China (English)

    庄文; 肖路加; 左凤琼; 东云华; 吴忠舜; 吴兆锋

    2002-01-01

    目的 探讨肝细胞膜钠泵(Na+-K+-ATPase)活性变化在胆红素钙结石成石过程中的作用.方法采用胆红素钙结石兔模型进行研究,对照组28只,胆道不全梗阻组(BO组)36只,胆道不全梗阻加感染组(BOI组)39只,各组再按术后处死时间3 d、7 d、14 d及20 d时相分组.动态测定各组各时相肝细胞膜钠泵活性、肝细胞钙及胆汁中胆汁酸含量. 结果 BO组及BOI组肝细胞膜钠泵活性及胆汁中胆汁酸含量进行性下降,肝细胞钙含量呈进行性增多,与对照组相比差异有显著性意义(P<0.01),但BOI组上述变化较BO组明显(P<0.05).结论胆红素钙结石成石过程中存在肝细胞膜钠泵活性的进行性下降,钠泵活性下降引起肝细胞钙超负荷及胆汁中胆汁酸含量下降,从而促进成石.细菌感染加重上述变化,使结石形成增多.

  19. Neonatal Dubin-Johnson syndrome: novel compound heterozygous mutation in the ABCC2 gene.

    Science.gov (United States)

    Okada, Hitoshi; Kusaka, Takashi; Fuke, Noriko; Kunikata, Jun; Kondo, Sonoko; Iwase, Takashi; Nan, Wang; Hirota, Takeshi; Ieiri, Ichiro; Itoh, Susumu

    2014-10-01

    Dubin-Johnson syndrome (DJS) is an autosomal recessive inherited disorder characterized by conjugated hyperbilirubinemia. Neonatal-onset DJS is rare. It is caused by dysfunction of adenosine triphosphate-binding cassette, sub-family C, member 2 (ABCC2). We found a novel compound heterozygous mutation of DJS-related gene: W709R (T2145C): a missense mutation in exon 17, and R768W (C2302T), a missense mutation in exon 18. Serum diglucuronosyl bilirubin/monoglucuronosyl bilirubin ratio was high. ABCC2 may excrete diglucuronosyl bilirubin preferentially over monoglucuronosyl bilirubin.

  20. A product of heme catabolism modulates bacterial function and survival.

    Directory of Open Access Journals (Sweden)

    Christopher L Nobles

    Full Text Available Bilirubin is the terminal metabolite in heme catabolism in mammals. After deposition into bile, bilirubin is released in large quantities into the mammalian gastrointestinal (GI tract. We hypothesized that intestinal bilirubin may modulate the function of enteric bacteria. To test this hypothesis, we investigated the effect of bilirubin on two enteric pathogens; enterohemorrhagic E. coli (EHEC, a Gram-negative that causes life-threatening intestinal infections, and E. faecalis, a Gram-positive human commensal bacterium known to be an opportunistic pathogen with broad-spectrum antibiotic resistance. We demonstrate that bilirubin can protect EHEC from exogenous and host-generated reactive oxygen species (ROS through the absorption of free radicals. In contrast, E. faecalis was highly susceptible to bilirubin, which causes significant membrane disruption and uncoupling of respiratory metabolism in this bacterium. Interestingly, similar results were observed for other Gram-positive bacteria, including B. cereus and S. aureus. A model is proposed whereby bilirubin places distinct selective pressure on enteric bacteria, with Gram-negative bacteria being protected from ROS (positive outcome and Gram-positive bacteria being susceptible to membrane disruption (negative outcome. This work suggests bilirubin has differential but biologically relevant effects on bacteria and justifies additional efforts to determine the role of this neglected waste catabolite in disease processes, including animal models.

  1. Systems pharmacology modeling of drug‐induced hyperbilirubinemia: Differentiating hepatotoxicity and inhibition of enzymes/transporters

    Science.gov (United States)

    Battista, C; Woodhead, JL; Stahl, SH; Mettetal, JT; Watkins, PB; Siler, SQ; Howell, BA

    2017-01-01

    Elevations in serum bilirubin during drug treatment may indicate global liver dysfunction and a high risk of liver failure. However, drugs also can increase serum bilirubin in the absence of hepatic injury by inhibiting specific enzymes/transporters. We constructed a mechanistic model of bilirubin disposition based on known functional polymorphisms in bilirubin metabolism/transport. Using physiologically based pharmacokinetic (PBPK) model‐predicted drug exposure and enzyme/transporter inhibition constants determined in vitro, our model correctly predicted indinavir‐mediated hyperbilirubinemia in humans and rats. Nelfinavir was predicted not to cause hyperbilirubinemia, consistent with clinical observations. We next examined a new drug candidate that caused both elevations in serum bilirubin and biochemical evidence of liver injury in rats. Simulations suggest that bilirubin elevation primarily resulted from inhibition of transporters rather than global liver dysfunction. We conclude that mechanistic modeling of bilirubin can help elucidate underlying mechanisms of drug‐induced hyperbilirubinemia, and thereby distinguish benign from clinically important elevations in serum bilirubin. PMID:28074467

  2. Genetics Home Reference: Rotor syndrome

    Science.gov (United States)

    ... of these proteins. Without the function of either transport protein, bilirubin is less efficiently taken up by the ... Schinkel AH. Complete OATP1B1 and OATP1B3 deficiency causes human Rotor syndrome by interrupting conjugated bilirubin reuptake into ...

  3. Genoptraeden af kernikterus hos nyfødte børn i Danmark

    DEFF Research Database (Denmark)

    Bjerre, Jesper V.; Ebbesen, Finn

    2006-01-01

    . The maximum plasma total bilirubin concentration was 531-745 mol/l. The aetiology was determined in two infants: spherocytosis and galactosaemia. Most likely, ABO blood type immunization was the reason in four cases. In two cases, the aetiology was unknown. Seven infants had symptoms of chronic bilirubin...

  4. Development and Application of a Method for Toxicological Assessment of Occupational Exposures to Chemicals in Marine Operations. Addendum.

    Science.gov (United States)

    1985-09-01

    protects against hepat- ic necrosis induced by carbon tetrachloride and against acute necrosis of the biliary epithelium following administration of a...retention of bile salts and bilirubin. The jaundice, resulting from retention of bilirubin, resembles that produced by extrahepatic biliary obstruction... ileus bromide Cholinesterase Transient muscle weakness inhibitors N- (pesticides) Magnesium sulfate Central depression and neuromuscular block Quinine

  5. Hyperbilirubinemia as a predictor for appendiceal perforation

    DEFF Research Database (Denmark)

    Burcharth, J; Pommergaard, Hans-Christian; Rosenberg, J;

    2013-01-01

    Delayed or wrong diagnosis in patients with appendicitis can result in perforation and consequently increased morbidity and mortality. Serum bilirubin may be a useful marker for appendiceal perforation. The purpose of this systematic review was to evaluate studies investigating elevated serum...... bilirubin as a predictor for appendiceal perforation....

  6. Extracorporeal albumin dialysis with the molecular adsorbent recirculating system in acute-on-chronic liver failure

    DEFF Research Database (Denmark)

    Bañares, Rafael; Nevens, Frederik; Larsen, Fin Stolze;

    2013-01-01

    .87, 95% confidence interval [CI] 0.44-1.72). MELD score and HE at admission and the increase in serum bilirubin at day 4 were independent predictors of death. At day 4, a greater decrease in serum creatinine (P=0.02) and bilirubin (P=0.001) and a more frequent improvement in HE (from grade II-IV to grade...

  7. Effects of thyroid status and thyrostatic drugs on hepatic glucuronidation of lodothyronines and other substrates in rats - Induction of phenol UDP-glucuronyltransferase by methimazole

    NARCIS (Netherlands)

    T.J. Visser (Theo); E. Kaptein (Ellen); A.L. Gijzel (Anthonie); W.W. de Herder (Wouter); M.L. Cannon (Mark); F. Bonthuis (Fred); W.J. de Greef (W.)

    1996-01-01

    textabstractGlucuronidation of iodothyronines in rat liver is catalyzed by at least three UDP-glucuronyltransferases (UGTs): bilirubin UGT, phenol UGT, and androsterone UGT. Bilirubin and phenol UGT activities are regulated by thyroid hormone, but the effect of thyroid status on hepatic glucuronidat

  8. New Evidences of Key Factors Involved in "Silent Stones" Etiopathogenesis and Trace Elements: Microscopic, Spectroscopic, and Biochemical Approach.

    Science.gov (United States)

    Cavalu, Simona; Popa, Adriana; Bratu, Ioan; Borodi, Gheorghe; Maghiar, Adrian

    2015-12-01

    The knowledge of the key factors involved in etiopathogenesis of the gallstone disease requires chemical, structural, and elemental composition analysis. The application of different complementary analytical techniques, both microscopic and spectroscopic, are aimed to provide a more comprehensive determination of the gallbladder calculi ultrastructure and trace element identification. High sensitivity techniques such as electron microscopy (SEM), Fourier transform infrared (FTIR), electron paramagnetic resonance (EPR) spectroscopy, and X-ray diffraction (XRD) along with biochemical analysis are used in a new attempt to investigate various factors which play a regulatory role in the pathogenesis of gallstones. The microstructure of different types of gallbladder stones has specific characteristics which are related to the elemental composition. The binding of metal ions with bile salts and bilirubin plays important roles in gallstone formation as revealed by FTIR spectrum of calcium bilirubinate complex in pigment gallstones. The EPR results demonstrated the generation of bilirubin free radicals and variation of its electronic structure and conjugation system in the skeleton of bilirubin molecule during complex formation. EPR spectra of pigment gallstones demonstrate the coexistence of four paramagnetic centers including stable bilirubin free radical, Mn2+, Cu2+, and Fe3+ with distinct magnetic parameters and well-resolved hyperfine structure in the case of Mn2+ ions. The result confirms a macromolecular network structure with proteins and the formation of bilirubin-coordinated polymer. Bilirubin and bilirubinate free radical complexes may play an important role in pigment gallstone formation.

  9. Bereiding van en onderzoek met "primaire" bilirubinestandaarden

    NARCIS (Netherlands)

    Dreumel; H.J. van; Wikkeling; R.H.; Phielix-Strubbe; C.J.; Klaassen; R.; Boink; A.B.T.J.; Koedam; J.C.

    1987-01-01

    De bereiding van primaire bilirubine-standaarden is mogelijk mits ; - gebruik wordt gemaakt van de beste kwaliteit bilirubine ; - HSA (en niet BSA) wordt gebruikt, dat direct voor gebruik wordt gefil- treerd ; - alle bewerkingen gebeuren onder strenge "donkere-kamer" condities ; - de

  10. Composition of common bile duct stones in Chinese patients during and after endoscopic sphincterotomy

    Institute of Scientific and Technical Information of China (English)

    Wei-Lun Tsai; Kwok-Hung Lai; Chiun-Ku Lin; Hoi-Hung Chan; Ching-Chu Lo; Ping-I Hsu; Wen-Chi Chen; Jin-Shiung Cheng; Gin-Ho Lo

    2005-01-01

    AIM: Endoscopic sphincterotomy (ES) is a well-established therapeutic modality for the removal of common bile duct (CBD) stones. After ES there are still around 10% of patients that experience recurrent CBD stones. The aim of this study is to investigate the composition of CBD stones before and after ES and its clinical significance in Chinese patients.METHODS: From January 1996 to December 2003, 735Veterans General Hospital and stone specimens from 266patients were sent for analysis. Seventy-five patients had recurrent CBD stones and stone specimens from 44patients were sent for analysis. The composition of the stones was analyzed by infrared (IR) spectrometry and they were classified as cholesterol or bilirubinate stones according to the predominant composition. Clinical data were analyzed.RESULTS: In the initial 266 stone samples, 217 (82%)were bilirubinate stones, 42 (16%) were cholesterol stones,3 were calcium carbonate stones, 4 were mixed cholesterol and bilirubinate stones. Patients with bilirubinate stones were significantly older than patients with cholesterol stones (66±13 years vs 56±17 years, P= 0.001). In the 44 recurrent stone samples, 38 (86%) were bilirubinate stones, 3 (7%) were cholesterol stones, and 3 were mixed cholesterol and bilirubinate stones. In 27 patients, bothinitial and recurrent stone specimens can be obtained,23 patients had bilirubinate stones initially and 2 became cholesterol stones in the recurrent attack. In the four patients with initial cholesterol stones, three patients had bilirubinate stones and one patient had a cholesterol stone in the recurrent attack.CONCLUSION: Bilirubinate stone is the predominant composition of initial or recurrent CBD stone in Chinese patients. The composition of CBD stones may be different from initial stones after ES.

  11. Hyperbilirubinemia after extracorporeal circulation surgery: A recent and prospective study

    Institute of Scientific and Technical Information of China (English)

    Yong An; Ying-Bin Xiao; Qian-Jin Zhong

    2006-01-01

    AIM: To clarify the incidence and nature of postoperative hyperbilirubinemia in patients after modern extracorporeal circulation, to analyze possible perioperative risk factors, and to elucidate the clinical significance of postoperative hyperbilirubinemia associated morality and morbidity.METHODS: Between March 2005 and May 2006, three hundred and eighty six consecutive patients undergoing extracorporeal circulation surgery due to a variety of cardiac lesions were investigated prospectively. The incidence of postoperative hyperbilirubinemia was defined as a serum total bilirubin concentration of more than 51 μmol/L. Several perioperative parameters were compared by logistic regression between hyperbilirubinemia and non-hyperbilirubinemia patients to determine possible risk factors contributing to postoperative hyperbilirubinemia and mortality.RESULTS: Overall incidence of postoperative hyperbilirubinemia was 25.3% (98/386). In patients with postoperative hyperbilirubinemia, 56.2%reached peak total bilirubin concentration on the first postoperative day, 33.5% on the second day, and 10.3%on the seventh day. Eighty percent of the increase of total bilirubin resulted from an increase of both conjugated and unconjugated bilirubin. Development of postoperative hyperbilirubinemia was associated with a higher mortality (P < 0.01), longer duration of mechanical ventilation (P < 0.05) and longer ICU stay time (P < 0.05). Preoperative total bilirubin concentration, preoperative right atrium pressure,numbers of valves replaced and of blood transfusion requirement were identified as important predictors for postoperative hyperbilirubinemia.CONCLUSION: Early postoperative hyperbilirubinemia after modern extracorporeal circulation is mainly caused by an increase in both conjugated and unconjugated bilirubin, and is associated with a high mortality.Important contributing factors are the preoperative total bilirubin concentration, preoperative severity of right atrial

  12. Novel diode-based laser system for combined transcutaneous monitoring and computer-controlled intermittent treatment of jaundiced neonates

    Science.gov (United States)

    Hamza, Mostafa; El-Ahl, Mohammad H. S.; Hamza, Ahmad M.

    2001-06-01

    The high efficacy of laser phototherapy combined with transcutaneous monitoring of serum bilirubin provides optimum safety for jaundiced infants from the risk of bilirubin encephalopathy. In this paper the authors introduce the design and operating principles of a new laser system that can provide simultaneous monitoring and treatment of several jaundiced babies at one time. The new system incorporates diode-based laser sources oscillating at selected wavelengths to achieve both transcutaneous differential absorption measurements of bilirubin concentration in addition to the computer controlled intermittent laser therapy through a network of optical fibers. The detailed description and operating characteristics of this system are presented.

  13. Study on the Property of

    Institute of Scientific and Technical Information of China (English)

    MAO; LuYuan

    2001-01-01

    How to utilize functional material to eliminate uncombined bilirubin is a key problem in the research of artificial hepatic supporter [1]. There have been reports on the utilization of cyclodextrin and its polymer as biomedical materials [2,3]. In this paper, it was studied that the property of cyclodextrin polymers (MPS-CDEP) synthesized by us for the inclusion and adsorption of bilirubin. Bilirubin is the degradation product of heme (ferriprotoporohyrin IX), which body contain, and structures of both as follows:  ……

  14. Database of Autotransplants for Breast Cancer.

    Science.gov (United States)

    1996-12-01

    Herpes Zoster 13 Esophagus 54 Rash, pustules or abscesses not typical 14 Stomach of any of the above 15 Gallbladder and biliary tree (not hepatitis...280-555 ml/m 2/day 556-833 Ml/M2/day >833 ml/m 2/day without ileus  ml/m 2/day 553. Liver: 1 U] Bilirubin 2 U] Bilirubin 3 U] Bilirubin 4 U...the above 15 Gallbladder and biliary tree (not hepatitis), pancreas 60 Central venous catheter, not otherwise specified 16 Small intestine 61

  15. Synthesis of 8-(ω-Chloroalkyl)-dipyrrinones

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    @@ Dipyrrinones,typically bright yellow compounds with a long axis-polarized π-π* excitation in the 14π-electron-conjugated chromophore,such as xanthobilirubic acid methyl ester(XBRMe),are useful building blocks for the syntheses of biologically important tetrapyrrole pigments such as bilirubin(the yellow pigment of jaundice)[1],and they have been extensively used as model dipyrrole pigments for the spectroscopic and the photochemical studies of liner tetrapyrroles such as bilirubin and biliverdin.They have also served as useful adjuncts in the studies of jaundice phototherapy and in bilirubin structure-biological function relationships[2].

  16. Transcutaneous bilirubinometry in the newborn infant: state of the art.

    Science.gov (United States)

    Hegyi, T

    1986-01-01

    Hyperbilirubinemia in the newborn infant continues to challenge physicians. Clinical evaluation and treatment have evolved well-established principles over the past decade. This review examines neonatal bilirubin metabolism and focuses on a recently developed clinical diagnostic tool, the transcutaneous bilirubinometer. In spite of some limitations, the transcutaneous bilirubinometer can be best applied as a screening tool to identify healthy full-term infants who require serum bilirubin determination. With proper application, this device can eliminate most invasive diagnostic testing. Optimal use of the instrument requires the relationship between the serum bilirubin concentration and the transcutaneous bilirubinometer index to be determined for each device, institution, and population.

  17. Role of extrahepatic UDP-glucuronosyltransferase 1A1: advances in understanding breast milk-induced neonatal hyperbilirubinemia

    Science.gov (United States)

    Fujiwara, Ryoichi; Maruo, Yoshihiro; Chen, Shujuan; Tukey, Robert H.

    2015-01-01

    Newborns commonly develop physiological hyperbilirubinemia (also known as jaundice). With increased bilirubin levels being observed in breast-fed infants, breast-feeding has been recognized as a contributing factor for the development of neonatal hyperbilirubinemia. Bilirubin undergoes selective metabolism by UDP-glucuronosyltransferase (UGT) 1A1 and becomes a water soluble glucuronide. Although several factors such as gestational age, dehydration and weight loss, and increased enterohepatic circulation have been associated with breast milk-induced jaundice (BMJ), deficiency in UGT1A1 expression is a known cause of BMJ. It is currently believed that unconjugated bilirubin is metabolized mainly in the liver. However, recent findings support the concept that extrahepatic tissues, such as small intestine and skin, contribute to bilirubin glucuronidation during the neonatal period. We will review the recent advances made towards understanding biological and molecular events impacting BMJ, especially regarding the role of extrahepatic UGT1A1 expression. PMID:26342858

  18. Breast milk jaundice

    Science.gov (United States)

    ... page: //medlineplus.gov/ency/article/000995.htm Breast milk jaundice To use the sharing features on this ... otherwise healthy, the condition may be called "breast milk jaundice." Causes Bilirubin is a yellow pigment that ...

  19. Hepatology may have problems with putative surrogate outcome measures

    DEFF Research Database (Denmark)

    Gluud, Christian; Brok, Jesper; Gong, Yan;

    2007-01-01

    hepatitis C, serum bilirubin concentration following ursodeoxycholic acid or immunosuppressants for patients with primary biliary cirrhosis, and nutritional outcomes following artificial nutrition for liver patients may not be valid surrogates for morbidity or mortality. The challenge is to develop reliable...

  20. Pro-C5, a marker of true type V collagen formation and fibrillation, correlates with portal hypertension in patients with alcoholic cirrhosis

    DEFF Research Database (Denmark)

    Leeming, Diana J; Veidal, Sanne S; Karsdal, Morten A;

    2015-01-01

    was combined in a linear regression model. RESULTS: Plasma Pro-C5 correlated to HVPG, indocyanine green clearance, sustained vascular resistance and mean arterial pressure (r = -0.68-0.33, p bilirubin and model for end...

  1. Jaundice in Healthy Newborns

    Science.gov (United States)

    ... 25 mg) that is not treated can cause deafness, cerebral palsy , or other forms of brain damage. ... the infant's red blood cells. This creates a sudden buildup of bilirubin in the baby's blood. Incompatibility ...

  2. Prospective association of liver function biomarkers with development of hepatobiliary cancers

    NARCIS (Netherlands)

    Stepien, Magdalena; Fedirko, Veronika; Duarte-Salles, Talita; Ferrari, Pietro; Freisling, Heinz; Trepo, Elisabeth; Trichopoulou, Antonia; Bamia, Christina; Weiderpass, Elisabete; Olsen, Anja; Tjønneland, Anne; Overvad, Kim; Boutron-Ruault, Marie Christine; Fagherazzi, Guy; Racine, Antoine; Kühn, Tilman; Kaaks, Rudolf; Aleksandrova, Krasimira; Boeing, Heiner; Lagiou, Pagona; Benetou, Vassiliki; Trichopoulos, Dimitrios; Palli, Domenico; Grioni, Sara; Tumino, Rosario; Naccarati, Alessio; Panico, Salvatore; Bueno-de-Mesquita, H. Bas; Peeters, Petra H.; Lund, Eiliv; Quirós, J. Ramón; Nápoles, Osmel Companioni; Sánchez, María José; Dorronsoro, Miren; Huerta, José María; Ardanaz, Eva; Ohlsson, Bodil; Sjöberg, Klas; Werner, Mårten; Nystrom, Hanna; Khaw, Kay Tee; Key, Timothy J.; Gunter, Marc; Cross, Amanda; Riboli, Elio; Romieu, Isabelle; Jenab, Mazda

    2016-01-01

    Introduction: Serum liver biomarkers (gamma-glutamyl transferase, GGT; alanine aminotransferase, ALT; aspartate aminotransferase, AST; alkaline phosphatase, ALP; total bilirubin) are used as indicators of liver disease, but there is currently little data on their prospective association with risk of

  3. Bereiding van bilirubinestandaarden

    NARCIS (Netherlands)

    Dreumel; H.J.van; Bosman; H.A.; Derks; H.J.G.M.; Hagen-Fast; A.K.; Koedam; J.C.; Klaasen; R.; Phielix-Strubbe; C.J.; Terlingen; J.B.A.

    1986-01-01

    Dit rapport beschrijft de bereiding van vijf partijen gevriesdroogde bilirubinestandaard. De volumeverandering die optreedt tengevolge van droogvriezen en reconstitutie kon worden berekend aan de hand van bepalingen van bilirubine, kalium, chloride en radioactiviteitsmetingen in de oorspronkelij

  4. Thumb Imprint Based Detection of Hyperbilirubinemia Using Luminescent Gold Nanoclusters

    Science.gov (United States)

    Basu, Srestha; Sahoo, Amaresh Kumar; Paul, Anumita; Chattopadhyay, Arun

    2016-12-01

    Early and easy detection of diseases, using point-of-care and inexpensive devices, not only provides option for early treatment but also reduces the risk of propagation. Herein we report the fabrication of a robust film based luminescence indicator of bilirubin, which can indicate hyperbilirubinemia through the thumb imprint of the patient. The UV-light induced luminescence intensity of the film, made out of chitosan stabilised gold (Au) nanoclusters, which was effectively quenched in the presence of Cu2+ ions, recovered in the presence of bilirubin from skin or blood serum. Moreover, the sensitivity of detection of bilirubin was tuneable with the amount of Cu2+ added, thereby facilitating the detection of the desired concentration range of bilirubin.

  5. A case of Gilbert's syndrome combined with macroamylasemia.

    Science.gov (United States)

    Inoue, H; Adachi, Y; Yamashita, M; Nanno, T; Katoh, H; Enomoto, M; Suwa, M; Yamamoto, T

    1989-06-01

    A 30-year-old Japanese male, who had no remarkable family history, visited our hospital with a complaint of abdominal pain, and unconjugated hyperbilirubinemia and hyperamylasemia were observed. He showed negative hemolysis tests, positive nicotinic acid test, low hepatic bilirubin UDP-glucuronyltransferase activity, decreased bilirubin diglucuronide and increased bilirubin monoglucuronide in bile, and a decrease in serum bilirubin after phenobarbital administration. He also showed high serum amylase level, low urine amylase level, and low amylase-creatinine clearance ratio. Gel filtration of serum with Sephadex G-200 revealed the existence of macroamylase. Countercurrent immunoelectrophoresis proved binding of serum amylase to lambda type IgA. From these results, the case was diagnosed as Gilbert's syndrome combined with macroamylasemia.

  6. OXYTOCIN INDUCED NEONATAL HYPERBILIRUBINEMIA

    Directory of Open Access Journals (Sweden)

    Smita S.

    2015-05-01

    Full Text Available INTRODUCTION: Hyperbilirubinemia is one of the most common causes of health problems, observed in 60% of term and 80% of preterm infants in the first week of life . Hyperbilirubinemia leads to neurotoxicity in severe condition. Some studies suggests that liberal use of oxytocin for inducing labour is one of the factor which lead to neonatal hyperbilirubinemia. OBJECTIVE: To compare the effect of oxytocin and neonatal bilirubin levels with spontaneous vaginal delivery . MATERIALS AND METHOD S : 100 full term parturients were selected for this study. The subjects were divided into two groups. 50 healthy babies of women who had oxytocin induced labour and 50 healthy babies of women with normal vaginal delivery following spontaneous onset of labour formed the control group. Neon atal serum bilirubin was measured on day 1, 3 and 5 after delivery. Bilirubin was measured by spectrophotometry. Data was analysed in ms excel sheet using spss 19.0v. Statistical analysis was done by using unpaired‘t’ test. RESULTS: There was significant i ncrease in bilirubin level in oxytocin induced group compared to control group on day 1 and 3. There was insignificant increase in bilirubin level in oxytocin induced group on day 5. However the level of serum bilirubin is within normal limits as bilirubin level normally rises on till 4 th day and decreases thereafter. CONCLUSION: Neonatal hyperbilirubinemia may be due to oxytocin administration by continues IV infusion which results in erythrocyte swell and rupture. Increase in bilirubin level in oxytocin i nduced group is within physiological limits

  7. Ruolo dei citocromi P450 cerebrali nell’ossidazione della bilirubina: inducibilità ed attività

    OpenAIRE

    Gambaro, Sabrina Eliana

    2014-01-01

    Abstract Hyperbilirubinemia is the most common clinical diagnosis during neona- tal life. The neonatal jaundice may be physiological without any clinical consequence, or can lead to an acute form of bilirubin en- cephalopathy with minimal neurological damage, or to more severe and dangerous condition called kernicterus (permanent neurological sequelae). The Gunn rat is one of the models in which bilirubin encephalopathy was studied. It w...

  8. Preparation of Chitosan-coated Nylon Membranes and their Application as Affinity Membranes

    Institute of Scientific and Technical Information of China (English)

    Wei SHI; Feng Bao ZHANG; Guo Liang ZHANG

    2005-01-01

    Chitosan-coated nylon membranes which possess a large number of reactive groups of-CH2OH and -NH2 were prepared by coupling chitosan onto the nylon membrane. Then polylysine as ligand was also immobilized onto the composite membranes by 1, l′-carbonyldiimidazole activation to prepare affinity membranes for bilirubin adsorption. The results showed that these membranes exhibited high binding affinity capacities for bilirubin and the adsorption isotherm fitted the Freundlich model well.

  9. Plasma catecholamine level and portal venous pressure as guides to prognosis in patients with cirrhosis

    DEFF Research Database (Denmark)

    Tage-Jensen, U; Henriksen, Jens Henrik Sahl; Christensen, E;

    1988-01-01

    clinical and biochemical variables and survival. Forty-seven (58%) of the patients died during the follow-up period. Univariate analysis showed that plasma noradrenaline and adrenaline concentrations, portal pressure, indocyanine green clearance, serum sodium, bilirubin, and albumin concentrations......, and the presence of ascites or cardiovascular disease were of significant prognostic value. In a multivariate analysis (Cox regression model), plasma noradrenaline concentration, portal pressure, serum bilirubin concentration, and the presence of ascites and cardiovascular disease remained significant independent...

  10. Clinical experience with ursodeoxycholic acid (Urdoxa) in complex therapy of chronic viral hepatitis

    OpenAIRE

    E. V. Esaulenko; O. E. Nikitina; N. V. Dunaeva; A. N. Uskov; T. L. Mogilevets

    2011-01-01

    Patients with chronic virus hepatitis (32 patients, 13 with chronic hepatitis B and 19 with chronic hepatitis C) ages from 20 to 72 with elevated levels of bilirubin and active alanine aminotransferase, aspartate aminotransferase, gamma- glutamyl transpeptidase, received ursodeoxycholic acid (Urdoxa) over the course of 12 weeks. During therapy alanine aminotransferase, aspartate aminotransferase, bilirubin and gamma-glutamyl transpeptidase levels decreased. Urdoxa demonstrated good tolerance,...

  11. Combination Antiangiogenic and Inmmunomodulatory Gene Therapy for Breast Cancer

    Science.gov (United States)

    2000-06-01

    Gastroenterology , Lawrence J. Brandt (ed.), Current Medicine, 1998. 2. Five chapters in UpToDate : B. Sauter, N. Roy Chowdhury, and J. Roy Chowdhury...InUpToDate, Burton D. Rose (ed.), UpToDate , Inc.; submitted. 1. Bilirubin Metabolism 2. Jaundice: Causes, Classifications and Approach to Diagnosis 3...Conjugated Bilirubin): Dubin Johnson Syndrome, Rotor Syndrome N.B.: UpToDate is a CD ROM book aimed at the "intelllectually oriented subspecialist

  12. A Comparison of Y-Type and T-Type Metallic Bilateral Biliary Stents in Patients with Malignant Hilar Biliary Obstruction

    Energy Technology Data Exchange (ETDEWEB)

    Koh, Esther; Jin, Gong Yong; Hwang, Seung Bae; Choi, Eun Jung; Song, Ji Soo; Han, Young Min; Kwon, Keun Sang [Dept. of Chonbuk National University Hospital and Medical School, Jeonju (Korea, Republic of)

    2013-04-15

    To compare the Y type (side-by-side) and T type (stent-in-stent) bilateral biliary metal stenting in malignant hilar obstruction in terms of treatment outcomes, including post-stenting serum bilirubin level and stent patency. 41 consecutive patients with advanced hilar malignancies who underwent percutaneous placement of bilateral metallic stents - Y (n = 23) and T types (n = 18) - were retrospectively reviewed. We evaluated stent patency after the procedure by cholangiogram and abdominal CT. Pre- and post-stenting serum bilirubin level (total, direct bilirubin) at 1 week and at 1 month were compared. Student t-test and Kaplan-Meier method were used in the statistical analysis. After comparing the median stent patency according to both types, they did not differ significantly (Y: 38 days, T: 61 days; p 0.141). There was a more decrease in the total and direct bilirubin of the T type compared to the Y type after 1 week (p = 0.013, 0.025). However, no significant difference existed between the decreasing bilirubin rates of both types after 1 month (p = 0.923, 0.742). In patients with malignant hilar obstruction, both Y and T type bilateral metallic biliary stents are effective methods. Stent patency and bilirubin decrease rates were not significantly different.

  13. The UGT1A1* allele and disease in childhood

    DEFF Research Database (Denmark)

    Padkær Petersen, Jesper

    Baggrund: Bilirubin er slutproduktet i hæms metabolisme. Ukonjugeret bilirubin er neuro-toksisk, men også en potent antioxidant af mulig klinisk relevans. Fortolkningen af observationelle bilirubin studier vanskeliggøres af potentiel revers kausalitet og adskillige tænkelige confoundere. En måde...... at adressere disse udfordringer på er at benytte UGT1A1*28 allelen som instrument variabel for bilirubin. Denne genetiske variation forklarer 18 % af alle variationer i serum bilirubin i kaukasiske populationer. Det er foreslået at UGT1A1*28 er en beskyttende antioxidativ genetisk adaptation, men den har også...... var UGT1A1*28 genotyper ikke associeret til akut respiratorisk sygdom, men en risikofaktor for varighed af respiratorisk sygdom Konklusion: Den manglende association mellem UGT1A1*28 genotyper og ALL hos børn indikerer at bilirubin ikke har nogen beskyttende antioxidant effekt mod denne sygdom. Den...

  14. Total Antioxidant Status in Patients with Major β-Thalassemia

    Directory of Open Access Journals (Sweden)

    Lili Koochakzadeh

    2011-06-01

    Full Text Available Objective:Beta-thalassemia major is an autosomal recessive disease causing severe and hemolytic anemia, which begins about 2-6 months after birth. Iron overload, which arises from recurrent transfusion and ineffective erythropoiesis, can enhance oxidative stress in thalassemic patients. The aim of this study was to evaluate the serum total antioxidant capacity of patients with ß-Thalassemia major. Methods:Sixty six Iranian patients with β-thalassemia major and 66 age-gender matched controls were evaluated for serum total antioxidant status (TAS, uric acid (UA, bilirubin and albumin. In addition, serum ferritin and transaminases were recorded in these subjects. Findings: Significant increases of TAS, UA, and bilirubin were observed in the patient group, compared with the control group (P<0.01. Mean TAS and bilirubin in male patients was higher than in females (P=0.005 and P=0.008, respectively. There was also direct correlation between TAS and albumin (P<0.001, bilirubin (P<0.001 and UA (P=0.002. Conclusion: Endogenous antioxidants such as ferritin, UA and bilirubin can result in increased level of TAS in the patients with Beta-thalassemia major. Compensatory excess of TAS to oxidative stress could also be the reason for difference between our findings and previous studies.

  15. Pancreatic islet regeneration and some liver biochemical parameters of leaf extracts of Vitex doniana in normal and streptozotocin-induced diabetic albino rats

    Institute of Scientific and Technical Information of China (English)

    Okpe Oche; Ibrahim Sani; Njoku Godwin Chilaka; Ndidi Uche Samuel; Atabo Samuel

    2014-01-01

    Objective: To test two water soluble extracts (aqueous and ethanolic) obtained from the leaves ofVitex doniana in normal and streptozotocin-induced diabetic rats for their effects on pancreatic endocrine tissues and serum marker enzymes for a period of 21 d. Methods: A total of 55 rats divided into 11 groups of 5 rats each were assigned into diabetic and non-diabetic groups and followed by a daily administration of ethanolic and aqueous extracts for 21 d. Group 1 was the normal control while group 7 was treated with standard drug.Results:The histopathological studies of the diabetic rats indicated increase in the volume density of islets, percent of β-cells and size of islet in the groups that received the plant extracts, which suggested regeneration of β-cells along with β-cells repairs, as compared with the non-treated diabetic control which showed complete degeneration of the islet cells. There was significant reduction (P0.01) in the serum activities of marker enzymes was observed for non-diabetic treated rats. Results of total bilirubin, direct bilirubin and unconjugated bilirubin showed that diabetic control group was significantly higher (P0.01) in total bilirubin and direct bilirubin compared with the normal control.Conclusion:This herbal therapy appears to bring about repair/regeneration of the endocrine pancreas and hepatic cells protection in the diabetic rat.

  16. Clinical Observation of Treatment of Hyperbilirubinemia in the Newborn by Integrated Traditional Chinese and Western Medicine

    Institute of Scientific and Technical Information of China (English)

    孟浦; 周东风; 赵萍; 夏传雄

    2002-01-01

    Objective: To find a method of treatment for correcting hyperbilirubinemia timely and effectively to prevent bilirubin induced cerebral damage in the newborn. Methods: The newborns with hyperbilirubinemia in the treated group were treated with conventional treatment plus Butyribacterial preparation (BBP) and Simo Decoction (SMD, 四磨饮) taken orally, and the effect was compared with that in control group A treated with conventional treatment only and that in control group B treated with conventional treatment plus BBP. Results: The mean daily decreasing rate of bilirubin in the treated group was 51.11±25.03 μmol/L, which was higher than that in control groups A (39.36±22.44 μmol/L) and B (43.24±24.18 μmol/L), respectively (P<0.01 and P<0.05). The bilirubin decreasing value on the first day and the speed of bilirubin decreasing (to 102.6 μmol/L) in the treated group were both higher than those in the control groups, P<0.01. Conclusion: The combined therapy of conventional treatment plus BBP and SMD could rapidly reduce the blood bilirubin level in the newborn with hyperbilirubinemia, and shorten the therapeutic course markedly, and the therapeutic effect is superior to that of the conventional treatment alone or that of the conventional treatment plus BBP only.

  17. 体内胆红素水平对高血压患者的影响

    Institute of Scientific and Technical Information of China (English)

    温晓俐; 赵兴胜

    2016-01-01

    Heme metabolism will produce bilirubin ,bilirubin in the role of heme oxygenase is decomposed into biliverdin biliverdin reductase role will change back to bilirubin .At the same time for patients with high blood pressure ,the level of bilirubin in the body will have an impact on patients . In this article ,the effects of different levels of bilirubin on the blood pressure in patients with hypertension were studied .%血红素代谢后会产生胆红素,胆红素在血红素氧合酶的作用下分解成胆绿素,胆绿素在还原酶的作用下会变回胆红素。同时对于高血压患者来说,体内胆红素的水平高低也会对患者产生影响。在本篇文章中,研究了体内胆红素水平的不同对高血压患者产生的影响。

  18. Calibrators and control samples for bilirubinometers.

    Science.gov (United States)

    Blijenberg, B G; Brügmann, G; Geilenkeuser, W J; Kusyschyn, R; Röhle, G; Schlebusch, H; Schneider, C

    1993-06-01

    The different matrix properties of neonatal serum and commercial control samples can lead to considerable errors in the calibration and control of bilirubinometers. These difficulties can be avoided by calibration with serum from healthy adults which is supplemented with unconjugated bilirubin. But this procedure is impracticable for most routine laboratories. Under certain preconditions, control samples, with bilirubin concentrations determined with correctly calibrated bilirubinometers or spectrophotometers, are also suitable as calibrators. This was established by determination of the bilirubin concentration of 16 different control samples, using both the reference method and correctly calibrated bilirubinometers or spectrophotometers in three or four specialist laboratories. This was also confirmed in several interlaboratory surveys, some involving up to 72 laboratories. The results of these investigations show that a control sample should be used for the calibration of a bilirubinometer only if it meets the following preconditions: 1. There should be no significant difference between the bilirubin values determined with the reference method and with a correctly calibrated spectrophotometer or bilirubinometer. 2. The bilirubin concentration should lie in the range 230-300 mumol/l. The photometric response of bilirubinometers has a limited linear range, so that analytical results greater than 300 mumol/l must be rated as basically unreliable.

  19. Rhesus isoimmunization: increased hemolysis during early infancy.

    Science.gov (United States)

    Hayde, M; Widness, J A; Pollak, A; Kohlhauser-Vollmuth, C; Vreman, H J; Stevenson, D K

    1997-05-01

    The objective of the present study was to determine whether whole blood carboxyhemoglobin (COHb) and plasma bilirubin, two indicators of hemolysis, are elevated in infants with severe Rh isoimmune hemolytic disease during the first months of life. Beginning at 2 wk of age and continuing monthly for 3 mo, serial blood samples were obtained for COHb, plasma bilirubin, Hb, reticulocyte count, plasma erythropoietin, plasma enzymes, and plasma iron. Because control infants (n = 13) and infants with ABO hemolytic disease (n = 5) did not differ from one another in any of the study parameters, these two groups were combined and compared with infants with the Rh isoimmunization. Infants with severe Rh isoimmune hemolytic disease (n = 13) were found to have significantly lower Hb and significantly higher bilirubin, the COHb fraction divided by the Hb concentration (COHb/Hb), and plasma erythropoietin levels at 2 and 6 wk of age, and reticulocyte counts at 6 wk. The remaining parameters were not different between the control-ABO group and Rh-isoimmune group at any of the study intervals. The study's two primary indicators of hemolysis, plasma bilirubin and COHb/Hb, were significantly correlated with one another in the Rh-immunized group (r = 0.66, p isoimmunization demonstrated a mean half-life of 14.3 d. We speculate that, among infants with severe Rh isoimmune hemolytic disease, elevated total bilirubin levels and COHb/Hb ratios identified in the early weeks of life indicate continuing hemolysis due to persistence of maternal Rh antibodies.

  20. Monitoring the Response of Hyperbilirubinemia in the Mouse Brain by In Vivo Bioluminescence Imaging.

    Science.gov (United States)

    Manni, Isabella; Di Rocco, Giuliana; Fusco, Salvatore; Leone, Lucia; Barbati, Saviana Antonella; Carapella, Carmine Maria; Grassi, Claudio; Piaggio, Giulia; Toietta, Gabriele

    2016-12-28

    Increased levels of unconjugated bilirubin are neurotoxic, but the mechanism leading to neurological damage has not been completely elucidated. Innovative strategies of investigation are needed to more precisely define this pathological process. By longitudinal in vivo bioluminescence imaging, we noninvasively visualized the brain response to hyperbilirubinemia in the MITO-Luc mouse, in which light emission is restricted to the regions of active cell proliferation. We assessed that acute hyperbilirubinemia promotes bioluminescence in the brain region, indicating an increment in the cell proliferation rate. Immunohistochemical detection in brain sections of cells positive for both luciferase and the microglial marker allograft inflammatory factor 1 suggests proliferation of microglial cells. In addition, we demonstrated that brain induction of bioluminescence was altered by pharmacological displacement of bilirubin from its albumin binding sites and by modulation of the blood-brain barrier permeability, all pivotal factors in the development of bilirubin-induced neurologic dysfunction. We also determined that treatment with minocycline, an antibiotic with anti-inflammatory and neuroprotective properties, or administration of bevacizumab, an anti-vascular endothelial growth factor antibody, blunts bilirubin-induced bioluminescence. Overall the study supports the use of the MITO-Luc mouse as a valuable tool for the rapid response monitoring of drugs aiming at preventing acute bilirubin-induced neurological dysfunction.

  1. Jaundice: a basic review

    Directory of Open Access Journals (Sweden)

    Muhammad Waseem Abbas

    2016-05-01

    Full Text Available Jaundice is a complex disease. Jaundice is actually the high bilirubin level in the body. Yellowing of skin, mucous membranes and skin are common presentations of jaundice. Jaundice has various variants including pre-hepatic jaundice (due to hemolysis of red blood cells, hepatic jaundice (due to defect in capture, conjugation and excretion of bilirubin by liver and post hepatic jaundice (due to the obstruction of extra hepatobiliary system. The causes of various variants of Jaundice is either acquired or congenital. High plasma bilirubin level can cause various manifestations involving satiety, gastrointestinal bleeding, diarrhea, anemia, edema, weight-loss and can be fatal because it can cause psychosis, lethargy, seizures, coma or even death. High bilirubin level can help in the diagnosis of Jaundice. Differential diagnosis of various variants of Jaundice can be carried out on the basis of bilirubin level (conjugated and unconjugated, ultrasonography and other radiological techniques. The proper management of Jaundice is high water intake and low fat diet. The primary effective treatment for pre-hepatic jaundice and neonatal physiological jaundice is phototherapy. Infusion of immunoglobulins is also used for treatment of pre-hepatic jaundice. Proper nutrition, steroids and immunosuppressant are used for treatment of hepatic jaundice. The treatment for post hepatic jaundice is decompression and surgery. [Int J Res Med Sci 2016; 4(5.000: 1313-1319

  2. Bile pigments in pulmonary and vascular disease

    Directory of Open Access Journals (Sweden)

    Stefan W. Ryter

    2012-03-01

    Full Text Available The bile pigments, biliverdin and bilirubin, are endogenously-derived substances generated during enzymatic heme degradation. These compounds have been shown to act as chemical antioxidants in vitro. Bilirubin formed in tissues circulates in the serum, prior to undergoing hepatic conjugation and biliary excretion. The excess production of bilirubin has been associated with neurotoxicity, in particular to the newborn. Nevertheless, clinical evidence suggests that mild states of hyperbilirubinemia may be beneficial in protecting against cardiovascular disease in adults. Pharmacological application of either bilirubin and/or its biological precursor biliverdin, can provide therapeutic benefit in several animal models of cardiovascular and pulmonary disease. Furthermore, biliverdin and bilirubin can confer protection against ischemia/reperfusion injury and graft rejection secondary to organ transplantation in animal models. Several possible mechanisms for these effects have been proposed, including direct antioxidant and scavenging effects, and modulation of signaling pathways regulating inflammation, apoptosis, cell proliferation, and immune responses. The practicality and therapeutic-effectiveness of bile pigment application to humans remains unclear.

  3. Bile pigments in pulmonary and vascular disease.

    Science.gov (United States)

    Ryter, Stefan W

    2012-01-01

    The bile pigments, biliverdin, and bilirubin, are endogenously derived substances generated during enzymatic heme degradation. These compounds have been shown to act as chemical antioxidants in vitro. Bilirubin formed in tissues circulates in the serum, prior to undergoing hepatic conjugation and biliary excretion. The excess production of bilirubin has been associated with neurotoxicity, in particular to the newborn. Nevertheless, clinical evidence suggests that mild states of hyperbilirubinemia may be beneficial in protecting against cardiovascular disease in adults. Pharmacological application of either bilirubin and/or its biological precursor biliverdin, can provide therapeutic benefit in several animal models of cardiovascular and pulmonary disease. Furthermore, biliverdin and bilirubin can confer protection against ischemia/reperfusion injury and graft rejection secondary to organ transplantation in animal models. Several possible mechanisms for these effects have been proposed, including direct antioxidant and scavenging effects, and modulation of signaling pathways regulating inflammation, apoptosis, cell proliferation, and immune responses. The practicality and therapeutic-effectiveness of bile pigment application to humans remains unclear.

  4. Hereditary spherocytosis in a patient undergoing coronary artery bypass grafting with cardiopulmonary bypass--a case report.

    Science.gov (United States)

    Spegar, J; Riha, H; Kotulak, T; Vanek, T

    2015-01-01

    Hereditary spherocytosis is a genetically determined abnormality of red blood cells. It is the most common cause of inherited haemolysis in Europe and North America within the Caucasian population. We document a patient who underwent an aortocoronary bypass procedure on cardiopulmonary bypass. In view of the uncertain tolerance of the abnormal red cells in hereditary spherocytosis to cardiopulmonary bypass, we reviewed the patient's chart and analyzed recorded values of these parameters: free plasma haemoglobin, renal parameters, cystatin C, bilirubin, liver tests, urine samples. From the results, we can see that slight haemolysis-elevated bilirubin in the blood sample and elevated bilirubin and urobilinogen in the urine sample occurred on the first postoperative day. The levels of these parameters slowly decreased during the next postoperative days. There was no real clinical effect of this haemolysis on renal functions.

  5. Efficacy of zinc sulfate in reducing unconjugated hyperbilirubinemia in neonates

    Directory of Open Access Journals (Sweden)

    Somayyeh Hashemian

    2014-09-01

    Full Text Available Hyperbilirubinemia is a common disease and unconjugated hyperbilirubinemia has been seen mainly in neonates. Severe form of unconjugated hyperbilirubinemia may cause kernicterus and even death. Conventional treatment for severe unconjugated hyperbilirubinemia consists of phototherapy and exchange transfusion that have several known disadvantages; specially exchange transfusion is associated with a significant morbidity and even mortality. These harmful effects indicate the need to develop alternative pharmacological treatment strategies for unconjugated hyperbilirubinemia. One of these pharmacological agents is zinc salts. Zinc has been shown to lower the bilirubin levels by inhibition of the enterohepatic cycling of unconjugated bilirubin. Oral zinc has been shown to reduce serum unconjugated bilirubin in animals, adolescents and low birth weight neonates. However, studies in healthy term neonates given oral zinc showed no reduction in hyperbilirubinemia based on daily measurement. In order to improve the accuracy, hyperbilirubinemia may be determined based on measurements every hour. More studies are needed to know the effect of zinc in neonatal jaundice.

  6. Follow-up of extreme neonatal hyperbilirubinaemia in 5- to 10-year-old children

    DEFF Research Database (Denmark)

    Vandborg, Pernille Kure; Hansen, Bo Moelholm; Greisen, Gorm;

    2015-01-01

    AIM: To investigate whether infants with neonatal hyperbilirubinaemia but without intermediate or advanced bilirubin encephalopathy develop long-term sequelae, with impairment of motor development, executive function, or hearing. METHOD: This nested double-cohort study included 167 exposed children...... spectrum disorder recorded in national registries. INTERPRETATION: No evidence was found of an increased risk of deficits in motor development, executive function, or hearing in children with extreme hyperbilirubinaemia who did not have intermediate or advanced bilirubin encephalopathy....... (107 males, 60 females) born in Denmark 2000 to 2005 at gestational age ≥35 weeks with a total serum bilirubin ≥450 μmol/L (26.3mg/dL) and 163 age-, sex-, and gestational age-matched unexposed children (103 males, 60 females). The children were examined at a mean age of 7.7 years (SD 1.7y) using...

  7. Fenofibrate treatment in two adults with Crigler-Najjar syndrome type II.

    Science.gov (United States)

    Yilmaz, Serif; Dursun, Mehmet; Canoruç, Fikri; Kidir, Veysel; Beştaş, Remzi

    2006-03-01

    Crigler-Najjar syndrome type II is a rare familial disorder of bilirubin conjugation with consecutive life-long unconjugated hyperbilirubinemia. In the presence of severe hyperbilirubinemia, a fetus or an adult is at risk for neurological defects in this syndrome. This paper is the first report emphasizing details about this disorder in two patients from Turkey. The diagnosis was made on the basis of history and laboratory findings excluding other causes of unconjugated hyperbilirubinemia. Phenobarbital loading test and C bile analysis also supported the diagnosis. There was a study in the literature in which treatment with chlofibrate had been recommended in this syndrome. Based on the results of that study, we administered fenofibrate treatment to our patients for one month and analyzed serum bilirubin levels before and after this procedure. No improvement in bilirubin levels was observed in either case.

  8. Effect of phototherapy with turquoise vs. blue LED light of equal irradiance in jaundiced neonates

    DEFF Research Database (Denmark)

    Ebbesen, Finn; Vandborg, Pernille K; Madsen, Poul H;

    2016-01-01

    for phototherapy is light emitting diodes (LEDs). AIM: Compare the bilirubin reducing effect in jaundiced neonates treated either with turquoise- or blue LED light with peak emission at 497 nm or 459 nm, respectively, with equal irradiance on the infants. METHODS: Infants with gestational age ≥33 weeks......) decrease of total serum bilirubin was 35.3% (32.5; 37.3) and 33.1% (27.1; 36.8) for infants treated with turquoise- and blue light, respectively. The difference was non-significant (p=0.53). The decrease was positively correlated to postnatal age and negatively to birth weight. CONCLUSION: Using LED light...... of equal irradiance, turquoise- and blue light had equal bilirubin reducing effect on hyperbilirubinemia of neonates.Pediatric Research (2015); doi:10.1038/pr.2015.209....

  9. SELECTED LIVER AND KIDNEY BIOCHEMICAL PROFILES OF HYBRID CATFISH EXPOSED TO JATROPHA CURCAS LEAF DUST

    Directory of Open Access Journals (Sweden)

    Kabir M. Adamu

    2013-04-01

    Full Text Available This study investigated the effect of sublethal concentrations (7.50, 5.00, 2.50 and 0.00 gL (control of Jatropha curcas leaf dust on some liver and kidney biochemical profiles such as total protein, total bilirubin, total albumin and total globulin of hybrid catfish (Clarias gariepinus (♀ and Heterobranchus bidorsalis (♂ after a 14-day experimental period. The result was significantly different in the mean value of determined liver total bilirubin, while total protein, albumin and globulin were insignificantly different in the exposed fish compared with the control. The kidney revealed varying levels of insignificant difference in its level of total protein, total albumin, total bilirubin and total globulin. Therefore, this investigation has revealed that sublethal concentration of Jatropha curcas has no effect on the basic function of the determined biochemical profiles of hybrid catfish and that the changes were directly proportional to J. curcas concentration.

  10. Validity of diagnostic codes and laboratory measurements to identify patients with idiopathic acute liver injury in a hospital database

    DEFF Research Database (Denmark)

    Udo, Renate; Maitland-van der Zee, Anke H; Egberts, Toine C G;

    2016-01-01

    of liver enzyme values (ALT > 2× upper limit of normal (ULN); AST > 1ULN + AP > 1ULN + bilirubin > 1ULN; ALT > 3ULN; ALT > 3ULN + bilirubin > 2ULN; ALT > 10ULN) and (II) algorithms based on solely liver enzyme values (ALT > 3ULN + bilirubin > 2ULN; ALT > 10ULN). Hospital medical records were reviewed......PURPOSE: The development and validation of algorithms to identify cases of idiopathic acute liver injury (ALI) are essential to facilitate epidemiologic studies on drug-induced liver injury. The aim of this study is to determine the ability of diagnostic codes and laboratory measurements...... 32% (13/41) to 48% (43/90) with the highest PPV found with ALT > 2ULN. The PPV for (II) algorithms with liver test abnormalities was maximally 26% (150/571). CONCLUSIONS: The algorithm based on ICD-9-CM codes indicative of ALI combined with abnormal liver-related laboratory tests is the most...

  11. Causes of immune dysfunction in hyperbilirubinemia model rats

    Institute of Scientific and Technical Information of China (English)

    Xiao-Min Sun; Ping Kang; Ke Tao

    2015-01-01

    Objective:To explore the causes of immune dysfunction in neonatal rats with hyperbilirubinemia.Methods: A total of 60 newborn SD rats were equally randomized into normal saline (NS) group, LPS control group, bilirubin control group, low-dose group and high-dose group. After anesthesia, 0.1 mL NS was given to the NS and LPS control group and different doses of bilirubin for the other groups; 1 h later, the NS and bilirubin control group received the intraperitoneal injection of 0.05 mL NS and 1mg/kg LPS for the other groups. After 5 or 24 hours of model establishment, spleens were collected for detecting the expression levels of MyD88 and p-TAK1 protein and the spleen cells apoptosis by immunohistochemmistry and TUNEL method. After 24 hours of model establishment, serum inflammatory factors levels and T cell subsets distribution were determined by ELISA and flow cytometry.Results: In contrast to low-dose bilirubin, high-dose bilirubin could induce spleen cells apoptosis in coordination with LPS. After 5 hours of model establishment, compared with NS group, MyD88 expression level in low-dose group elevated while p-TAK1 level in high-dose group reduced (P<0.05). In high-dose group, inflammotory factors levels and CD8+ T cells percentage were all higher than LPS control and NS group (P<0.05), while CD4+ T cells percentage was lower than NS group (P<0.05).Conclusions:High-concentration plasma bilirubin in coordination with LPS could inhibit NF-κB signal pathways activation and aggravate inflammatory reaction, thus caused immunosuppression with inflammation cascade, which resulted in the immune dysfunction.

  12. 生理盐水灌肠治疗新生儿高胆红素血症的临床观察

    Institute of Scientific and Technical Information of China (English)

    张娟

    2015-01-01

    新生儿黄疸是新生儿时期常见症状之一,尤其是早期新生儿,其发生与新生儿胆红素产生增多、肝细胞功能不成熟、胆红素肠肝循环增加有关[1]。新生儿高胆红素血症多以未结合胆红素增高为主。未结合胆红素对神经细胞有毒性作用,它通过血脑屏障作用于脑细胞能引起脑损伤及中毒性脑病,可留有不同程度的神经系统后遗症,严重者甚至死亡[2]。临床上,以总胆红素水平及未结合胆红素水平作为胆红素脑病危险因素来指导新生儿胆红素血症的防治。%neonatal jaundice is one of the common symptoms of neonatal period,especially in early newborns.The occurrence and neonatal bilirubin have increased,liver cell function is not mature and enterohepatic circulation of bilirubin increased (1).The unconjugated hyperbilirubinemia increase.Unconjugated bilirubin can have toxic effects on nerve cells,which through the blood brain barrier in brain cells can cause brain injury and poisoning encephalopathy,there may be a different degree of neurological sequelae,serious and even death (2).Clinically,the serum levels of total bilirubin and unbound bilirubin levels as risk factors of bilirubin encephalopathy to guide the prevention and treatment of neonatal hyperbilirubinemia.

  13. Early Hepatic Dysfunction Is Associated with a Worse Outcome in Patients Presenting with Acute Respiratory Distress Syndrome: A Post-Hoc Analysis of the ACURASYS and PROSEVA Studies

    Science.gov (United States)

    Dizier, Stéphanie; Forel, Jean-Marie; Ayzac, Louis; Richard, Jean-Christophe; Hraiech, Sami; Lehingue, Samuel; Loundou, Anderson; Roch, Antoine; Guerin, Claude; Papazian, Laurent

    2015-01-01

    Introduction Bilirubin is well-recognized marker of hepatic dysfunction in intensive care unit (ICU) patients. Multiple organ failure often complicates acute respiratory distress syndrome (ARDS) evolution and is associated with high mortality. The effect of early hepatic dysfunction on ARDS mortality has been poorly investigated. We evaluated the incidence and the prognostic significance of increased serum bilirubin levels in the initial phase of ARDS. Methods The data of 805 patients with ARDS were retrospectively analysed. This population was extracted from two recent multicenter, prospective and randomised trials. Patients presenting with ARDS with a ratio of the partial pressure of arterial oxygen to the fraction of inspired oxygen < 150 mmHg measured with a PEEP ≥ 5 cm of water were included. The total serum bilirubin was measured at inclusion and at days 2, 4, 7 and 14. The primary objective was to analyse the bilirubin at inclusion according to the 90-day mortality rate. Results The 90-day mortality rate was 33.8% (n = 272). The non-survivors were older, had higher Sepsis-related Organ Failure Assessment (SOFA) score and were more likely to have a medical diagnosis on admission than the survivors. At inclusion, the SOFA score without the liver score (10.3±2.9 vs. 9.0±3.0, p<0.0001) and the serum bilirubin levels (36.1±57.0 vs. 20.5±31.5 μmol/L, p<0.0001) were significantly higher in the non-survivors than in the survivors. Age, the hepatic SOFA score, the coagulation SOFA score, the arterial pH level, and the plateau pressure were independently associated with 90-day mortality in patients with ARDS. Conclusion Bilirubin used as a surrogate marker of hepatic dysfunction and measured early in the course of ARDS was associated with the 90-day mortality rate. PMID:26636318

  14. Association of laboratory parameters with viral factors in patients with hepatitis C

    Directory of Open Access Journals (Sweden)

    Khaliq Saba

    2011-07-01

    Full Text Available Abstract Background and Aims HCV infection may lead to hepatic fibrosis. In this study, we tried to determine whether there is any correlation of HCV genotypes and viral load to the clinical parameters such as ALT, AST, ALP, bilirubin, Hb level, patient's age and gender; and then correlated this association with disease progression in liver biopsy samples. Methods In cross-sectional and observational study, 6048 serum HCV RNA positive patients were chosen. The study consists of 53 months from March 2006 to September 2010. Patients were divided into three cohorts to validate our data. Statistical analysis and correlation of lab parameters with viral factors was determined by using SPSS version 16. Results The most prevalent genotype was 3 (70.9% followed by 1 (13.3% and 4 (7.4%, collectively. During Univariate analysis, in all cohorts; serum bilirubin, ALP, ALT and AAR showed significant correlation with genotypes, however multivariate analysis showed that all genotypes except 4a have no association with host biochemical markers. Disease progression was also independent of all genotypes. Serum ALP, ALT, bilirubin and viremea levels were significantly elevated in patients with genotype 4a. Viral load showed negative association with serum bilirubin (r = -0.112, P = 0.000 and ALP levels (r = -0.098, P = 0.000. We observed positive correlation of ALP and bilirubin levels, while negative associations of viral load with HCV liver disease progression. Conclusion Disease progression seems independent of the genotypes. Relationship between ALP and bilirubin with viral load may be an attractive marker to guess disease progression in patients with hepatitis C.

  15. Lineage-Specific Changes in Biomarkers in Great Apes and Humans.

    Directory of Open Access Journals (Sweden)

    Claudius Ronke

    Full Text Available Although human biomedical and physiological information is readily available, such information for great apes is limited. We analyzed clinical chemical biomarkers in serum samples from 277 wild- and captive-born great apes and from 312 healthy human volunteers as well as from 20 rhesus macaques. For each individual, we determined a maximum of 33 markers of heart, liver, kidney, thyroid and pancreas function, hemoglobin and lipid metabolism and one marker of inflammation. We identified biomarkers that show differences between humans and the great apes in their average level or activity. Using the rhesus macaques as an outgroup, we identified human-specific differences in the levels of bilirubin, cholinesterase and lactate dehydrogenase, and bonobo-specific differences in the level of apolipoprotein A-I. For the remaining twenty-nine biomarkers there was no evidence for lineage-specific differences. In fact, we find that many biomarkers show differences between individuals of the same species in different environments. Of the four lineage-specific biomarkers, only bilirubin showed no differences between wild- and captive-born great apes. We show that the major factor explaining the human-specific difference in bilirubin levels may be genetic. There are human-specific changes in the sequence of the promoter and the protein-coding sequence of uridine diphosphoglucuronosyltransferase 1 (UGT1A1, the enzyme that transforms bilirubin and toxic plant compounds into water-soluble, excretable metabolites. Experimental evidence that UGT1A1 is down-regulated in the human liver suggests that changes in the promoter may be responsible for the human-specific increase in bilirubin. We speculate that since cooking reduces toxic plant compounds, consumption of cooked foods, which is specific to humans, may have resulted in relaxed constraint on UGT1A1 which has in turn led to higher serum levels of bilirubin in humans.

  16. Interaction of indomethacin with adult human albumin and neonatal serum

    DEFF Research Database (Denmark)

    Honoré, B; Brodersen, R; Robertson, A

    1983-01-01

    The binding of indomethacin to albumin was investigated at 37 degrees C, pH 7.4. The first stoichiometric binding constant is 2.5 X 10(5) M-1. Indomethacin utilizes both the bilirubin and diazepam binding functions equally. The effect on bilirubin binding to albumin is negligible at therapeutic i...... no significant correlation of the reserve binding to the albumin level. This methodology may be useful in studying the variable response of infants with patent ductus arteriosus to indomethacin. Udgivelsesdato: 1983-null...

  17. Surveillance of extreme hyperbilirubinaemia in Denmark. A method to identify the newborn infants

    DEFF Research Database (Denmark)

    Bjerre, J.V.; Petersen, Jes Reinholdt; Ebbesen, F.

    2008-01-01

    serum bilirubin concentration (TSB) > or = 450 micromol/L were obtained by linking laboratory data to the unique Danish personal identification number. RESULTS: In total, 113 infants were included, that is, an incidence of 45/100,000 live births. Thirty-seven infants presented in hospital, 2 after home...... bilirubin encephalopathy; one infant had advanced-phase symptoms. Four infants received an exchange transfusion. ABO blood group incompatibility was present in 52 infants. Thirty-seven infants were of non-Caucasian descent. CONCLUSION: A method to obtain the national epidemiological data is presented...

  18. Metalloporphyrins – an update

    Directory of Open Access Journals (Sweden)

    Stephanie eSchulz

    2012-04-01

    Full Text Available Metalloporphyrins are structural analogues of heme and their potential use in the management of neonatal hyperbilirubinemia has been the subject of considerable research for more than three decades. The pharmacological basis for using this class of compounds to control bilirubin levels is the targeted blockade of bilirubin production through the competitive inhibition of heme oxygenase (HO, the rate-limiting enzyme in the heme degradative pathway. Ongoing research continues in the pursuit of identifying ideal metalloporphyrins, which are safe and effective, by defining therapeutic windows and targeted interventions for the treatment of neonatal hyperbilirubinemia.

  19. Prognostic value of Child-Turcotte criteria in medically treated cirrhosis

    DEFF Research Database (Denmark)

    Christensen, E; Schlichting, P; Fauerholdt, L

    1984-01-01

    The Child- Turcotte criteria (CTC) (based on serum bilirubin and albumin, ascites, neurological disorder and nutrition) are established prognostic factors in patients with cirrhosis having portacaval shunt surgery. The objective of this study was to evaluate the prognostic value of CTC in conserv......The Child- Turcotte criteria (CTC) (based on serum bilirubin and albumin, ascites, neurological disorder and nutrition) are established prognostic factors in patients with cirrhosis having portacaval shunt surgery. The objective of this study was to evaluate the prognostic value of CTC...

  20. An Unusual Case of Rapidly Progressive Hyperbilirubinemia

    Directory of Open Access Journals (Sweden)

    Kimberly M. Thornton

    2013-01-01

    Full Text Available We present an unusual case of hyperbilirubinemia with rapid early progression leading to bilirubin encephalopathy in a term neonate. Despite early recognition and intervention, the total serum bilirubin reached a maximum level of 39 mg/dL at 32 hours of life. Prior to an emergent exchange transfusion, the patient’s diagnostic evaluation was significant for Coombs-negative microangiopathic hemolytic anemia and thrombocytopenia. Further testing revealed a deficiency of ADAMTS13 protein, or von Willebrand factor-cleaving protease, a finding diagnostic of congenital thrombotic thrombocytopenic purpura, or Upshaw-Schulman syndrome. This rare disease is often misdiagnosed, especially in the newborn period.

  1. Hemolysis in Preterm Neonates.

    Science.gov (United States)

    Christensen, Robert D; Yaish, Hassan M

    2016-06-01

    Hemolysis can be an important cause of hyperbilirubinemia in premature and term neonates. It can result from genetic abnormalities intrinsic to or factors exogenous to normal to red blood cells (RBCs). Hemolysis can lead to a relatively rapid increase in total serum/plasma bilirubin, hyperbilirubinemia that is somewhat slow to fall with phototherapy, or hyperbilirubinemia that is likely to rebound after phototherapy. Laboratory methods for diagnosing hemolysis are more difficult to apply, or less conclusive, in preterm infants. Transfusion of donor RBCs can present a bilirubin load that must be metabolized. Genetic causes can be identified by next-generation sequencing panels.

  2. Hyperbilirubinemia in newborns with gestation≥35 weeks%胎龄≥35周的新生儿高胆红素血症(摘译)

    Institute of Scientific and Technical Information of China (English)

    Koravangattu SANKARAN; 姚跃

    2010-01-01

    @@ 1 Introduction The prevention, detection and management of neonatal jaundice in relatively healthy term and near term infants continue to remain a challenge, partly because jaundice is so common, and kernicterus so rare[1-3]. The term ker-nicterus is now used interchangeably with both acute and chronic bilirubin toxicity and appears to have a current incidence of approximately 1:100 000[4-5]. In addition there are many other children who require intensive thera-py in order to interrupt the rise in serum bilirubin concen-trations[6].

  3. Carnitine deficiency with hyperbilirubinemia, generalized skeletal muscle weakness and reactive hypoglycemia in a patient on long-term total parenteral nutrition: treatment with intravenous L-carnitine.

    Science.gov (United States)

    Worthley, L I; Fishlock, R C; Snoswell, A M

    1983-01-01

    Low levels of plasma carnitine and reduced urinary carnitine excretion with persistently elevated plasma bilirubin levels, reactive hypoglycemia and generalized skeletal muscle weakness are described in a patient requiring long-term total parenteral nutrition (TPN). Intravenous administration of L-carnitine at 400 mg/day for 7 days and subsequently a maintenance dose of 60 mg/day corrected the plasma carnitine deficiency and reactive hypoglycemia and was associated with a return to normal plasma bilirubin levels and a restoration of skeletal muscle strength.

  4. Clinical rounds in the well-baby nursery: treating jaundiced newborns.

    Science.gov (United States)

    Maisels, M J

    1995-10-01

    Ten pearls (and pitfalls) in the management of the jaundiced newborn: Remember to take a history. Ask about jaundice in previous siblings and check family ethnicity. Don't ignore jaundice in the first 24 hours--it is considered pathologic until proven otherwise. Some normal infants may appear jaundiced and have a bilirubin level of 5 mg/dL at 23 hours and 59 minutes. On the other hand, a bilirubin level of 5 mg/dL at 10 hours is almost certainly pathologic. Use your judgment. Don't treat 35 to 37 week gestation infants as if they were full-term infants. Although these babies are cared for in well-baby nurseries and are generally treated like full-term infants, they are not full term. They are not as vigorous and do not nurse as well as full-term infants. Infants at 37 weeks gestation are four times more likely to have a serum bilirubin level greater than 13 mg/dL than those at 40 weeks gestation. Don't send 35-week gestation infants home before 48 hours. Document your assessment, particularly if the infant is being discharged early. Document the presence or absence of jaundice and its severity. A late rising bilirubin is typical of G6PD deficiency. Think about the ethnic background: G6PD deficiency is much more likely to occur in families from Greece, Turkey, Sardinia, and Nigeria, and particularly in Sephardic Jews from Iraq, Iran, Syria, and Kurdistan. Your practice may not contain many such families but remember in today's world of travel and intermarriage, etc, these genes are ubiquitous and the diagnosis of G6PD deficiency should always be considered in a newborn child with a significant elevation of bilirubin, particularly if it is a male and the rise in bilirubin is of late onset. Don't use homeopathic doses of phototherapy. As with any drug, phototherapy should be provided in a therapeutic dose (see above), but with the light sources commonly used, it is impossible to overdose the patient. Don't ignore a failure of response to phototherapy. If the bilirubin

  5. ESPGHAN Committee on Nutrition Position Paper. Intravenous lipid emulsions and risk of hepatotoxicity in infants and children

    DEFF Research Database (Denmark)

    Hojsak, Iva; Colomb, Virginie; Braegger, Christian

    2016-01-01

    be performed. Available studies found that the use of multicomponent fish oil (FO) containing ILE compared to pure soya bean oil (SO) ILE reduced liver enzymes and bilirubin levels in non-cholestatic children on long-term PN and one other RCT found that FO based ILE reversed cholestasis in a proportion...

  6. Reversal of Jaundice in Two Patients with Inoperable Cholangiocarcinoma Treated with Cisplatin and Gemcitabine Combination

    Directory of Open Access Journals (Sweden)

    Maarten Criel

    2012-01-01

    Full Text Available Two patients are presented with severe jaundice, due to inoperable cholangiocarcinoma. The chemotherapeutic approach in patients with severe jaundice is discussed. Many schedules of chemotherapy were developed in this tumor type with normal serum bilirubin. We report here the first successful use of cisplatin and gemcitabine combination chemotherapy in these patients. Tolerability was good and liver function tests gradually improved.

  7. Assessment of Requirement of Routine Intraoperative Cholaniography at Cholecystectomy

    Directory of Open Access Journals (Sweden)

    M Shiryazdi

    2013-12-01

    Conclusion: According to above statistically significant relation, we recommend that in patients who are candidate for cholecystectomy, if elevated preoperative serum alkaline phosphatase or elevated preoperative serum bilirubin or abnormal ultrasound findings were observed, more diagnostic evaluation should be done for them including MRC and ERC.

  8. cal, therapeutic and histo-morphological studies in primary biliary cirrhosis and primary sclerosing cholangitis

    NARCIS (Netherlands)

    H.J.F. Hoogstraten (Hubert)

    1999-01-01

    textabstractPrimary biliary cirrhosis (PBC) and primary sclerosing cholangitis (PSC) are chronic cholestatic liver diseases, biochemically characterised by an elevated serum bilirubin, alkaline phosphatase (APh) and y-glutamyl transferase (y-GT). Although PBC and PSC have their own distinctive clini

  9. Evanescent field: A potential light-tool for theranostics application

    Science.gov (United States)

    Polley, Nabarun; Singh, Soumendra; Giri, Anupam; Pal, Samir Kumar

    2014-03-01

    A noninvasive or minimally invasive optical approach for theranostics, which would reinforce diagnosis, treatment, and preferably guidance simultaneously, is considered to be major challenge in biomedical instrument design. In the present work, we have developed an evanescent field-based fiber optic strategy for the potential theranostics application in hyperbilirubinemia, an increased concentration of bilirubin in the blood and is a potential cause of permanent brain damage or even death in newborn babies. Potential problem of bilirubin deposition on the hydroxylated fiber surface at physiological pH (7.4), that masks the sensing efficacy and extraction of information of the pigment level, has also been addressed. Removal of bilirubin in a blood-phantom (hemoglobin and human serum albumin) solution from an enhanced level of 77 μM/l (human jaundice >50 μM/l) to ˜30 μM/l (normal level ˜25 μM/l in human) using our strategy has been successfully demonstrated. In a model experiment using chromatography paper as a mimic of biological membrane, we have shown efficient degradation of the bilirubin under continuous monitoring for guidance of immediate/future course of action.

  10. Severe Hemolysis in a Patient With Erythrocytosis During Coupled Plasma Filtration Adsorption Therapy Was Prevented by Changing From Membrane-Based Technique to a Centrifuge-Based One.

    Science.gov (United States)

    Fan, Rong; Wu, Buyun; Kong, Ling; Gong, Dehua

    2016-01-01

    Coupled plasma filtration adsorption (CPFA) usually adopts membrane to separate plasma from blood. Here, we reported a case with erythrocytosis experienced severe hemolysis and membrane rupture during CPFA, which was avoided by changing from membrane-based technique to a centrifuge-based one. A 66-year-old man was to receive CPFA for severe hyperbilirubinemia (total bilirubin 922 μmol/L, direct bilirubin 638 μmol/L) caused by obstruction of biliary tract. He had erythrocytosis (hemoglobin 230 g/L, hematocrit 0.634) for years because of untreated tetralogy of Fallot. Severe hemolysis and membrane rupture occurred immediately after blood entering into the plasma separator even at a low flow rate (50 mL/min) and persisted after changing a new separator. Finally, centrifugal plasma separation technique was used for CPFA in this patient, and no hemolysis occurred. After 3 sessions of CPFA, total bilirubin level decreased to 199 μmol/L with an average decline by 35% per session. Thereafter, the patient received endoscopic biliary stent implantation, and total bilirubin level returned to nearly normal. Therefore, centrifugal-based plasma separation can also be used in CPFA and may be superior to a membrane-based one in patients with hyperviscosity.

  11. Gallbladder bile composition in patients with Crohn's disease

    Institute of Scientific and Technical Information of China (English)

    Annika Lapidus; Jan-Erik (A)kerlund; Curt Einarsson

    2006-01-01

    AIM: To further elucidate the pathogenesis and mechanisms of the high risk of gallstone formation in Crohn's disease.METHODS: Gallbladder bile was obtained from patients with Crohn's disease who were admitted for elective surgery (17 with ileallileocolonic disease and 7 with Crohn's colitis). Fourteen gallstone patients served as controls. Duodenal bile was obtained from ten healthy subjects before and after the treatment with ursodeoxycholic acid. Bile was analyzed for biliary lipids,bile acids, bilirubin, crystals, and crystal detection time (CDT). Cholesterol saturation index was calculated.RESULTS: The biliary concentration of bilirubin was about 50% higher in patients with Crohn's disease than in patients with cholesterol gallstones. Ten of the patients with Crohn's disease involving ileum and three of those with Crohn's colitis had cholesterol saturated bile. Four patients with ileal disease and one of those with colonic disease displayed cholesterol crystals in their bile. About 1/3 of the patients with Crohn's disease had a short CDT. Treatment of healthy subjects with ursodeoxycholic acid did not increase the concentration of bilirubin in duodenal bile. Several patients with Crohn's disease,with or without ileal resection/disease had gallbladder bile supersaturated with cholesterol and short CDT and contained cholesterol crystals. The biliary concentration of bilirubin was also increased in patients with Crohn's colitis probably not due to bile acid malabsorption.CONCLUSION: Several factors may be of importance for the high risk of developing gallstones of both cholesterol and pigment types in patients with Crohn's disease.

  12. Veterinary Research Manpower Development for Defense

    Science.gov (United States)

    2011-09-01

    as a Function Population Management Strategies Gordon, Max Dr. Siobhan Mor Evaluating Cost-Effective Rabies Treatments in Uganda Hamilton, Jessie...Oximetry and Assessing its Usefulness in Detecting Decreased Pulmonary Function in Elephants with Tuberculosis Murch, Cecilia Dr. J. Lindenmayer...phosphorus, glucose, creatine kinase, uric acid, sodium, potassium, chloride, amylase , lipase, total cholesterol and direct bilirubin. Statistically

  13. Effective Treatment of Unconjugated Hyperbilirubinemia With Oral Bile Salts in Gunn Rats

    NARCIS (Netherlands)

    Cuperus, Frans J. C.; Hafkamp, Anja M.; Havinga, Rick; Vitek, Libor; Zelenka, Jaroslav; Tiribelli, Claudio; Ostrow, J. Donald; Verkade, Henkjan J.

    2009-01-01

    Background & Aims: we tested the hypothesis that oral administration of bile salts, which are known to increase the biliary excretion of unconjugated bilirubin (UCB), decreases unconjugated hyperbilirubinemia in the Gunn rat model. Methods: Adult Gunn rats were fed a standard diet or the same diet s

  14. Novel kinetic insights into treatment of unconjugated hyperbilirubinemia : Phototherapy and orlistat treatment in Gunn rats

    NARCIS (Netherlands)

    Hafkamp, AM; Havinga, R; Ostrow, JD; Tiribelli, C; Pascolo, L; Sinaasappel, M; Verkade, HJ

    2006-01-01

    Treatment with phototherapy or with the lipase inhibitor orlistat decreases plasma unconjugated bilirubin (UCB) concentrations in hyperbilirubinemic Gunn rats. We investigated the mechanism(s) underlying the effects of orlistat, phototherapy, and combined treatment, using steady-state 3H-UCB kinetic

  15. In vitro and in vivo efficacy of new blue light emitting diode phototherapy compared to conventional halogen quartz phototherapy for neonatal jaundice.

    Science.gov (United States)

    Chang, Yun Sil; Hwang, Jong Hee; Kwon, Hyuk Nam; Choi, Chang Won; Ko, Sun Young; Park, Won Soon; Shin, Son Moon; Lee, Munhyang

    2005-02-01

    High intensity light emitting diodes (LEDs) are being studied as possible light sources for the phototherapy of neonatal jaundice, as they can emit high intensity light of narrow wavelength band in the blue region of the visible light spectrum corresponding to the spectrum of maximal bilirubin absorption. We developed a prototype blue gallium nitride LED phototherapy unit with high intensity, and compared its efficacy to commercially used halogen quartz phototherapy device by measuring both in vitro and in vivo bilirubin photodegradation. The prototype device with two focused arrays, each with 500 blue LEDs, generated greater irradiance than the conventional device tested. The LED device showed a significantly higher efficacy of bilirubin photodegradation than the conventional phototherapy in both in vitro experiment using microhematocrit tubes (44+/-7% vs. 35+/-2%) and in vivo experiment using Gunn rats (30+/-9% vs. 16+/-8%). We conclude that high intensity blue LED device was much more effective than conventional phototherapy of both in vitro and in vivo bilirubin photodegradation. Further studies will be necessary to prove its clinical efficacy.

  16. The value of hyperbilirubinaemia in the diagnosis of acute appendicitis.

    LENUS (Irish Health Repository)

    Emmanuel, Andrew

    2011-04-01

    No reliably specific marker for acute appendicitis has been identified. Although recent studies have shown hyperbilirubinaemia to be a useful predictor of appendiceal perforation, they did not focus on the value of bilirubin as a marker for acute appendicitis. The aim of this study was to determine the value of hyperbilirubinaemia as a marker for acute appendicitis.

  17. Liver collagen in cirrhosis correlates with portal hypertension and liver dysfunction

    DEFF Research Database (Denmark)

    Nielsen, Kåre; Clemmesen, Jens Otto; Vassiliadis, Efstathios;

    2014-01-01

    livers. In 20 of the livers, CPA was measured in more than one tissue sample. CPA showed significant correlations with HVPG and with various surrogate markers of hepatic dysfunction including albumin, bilirubin, INR, MELD score and Child-Pugh score. CPA reliably discriminated HVPG ≥10 mmHg, termed...

  18. Gemtuzumab ozogamicin as postconsolidation therapy does not prevent relapse in children with AML

    DEFF Research Database (Denmark)

    Hasle, Henrik; Abrahamsson, Jonas; Forestier, Erik;

    2012-01-01

    weeks. We randomized 120 patients; 59 to receive GO. Survival was analyzed on an intention-to-treat basis. The median follow-up for patients who were alive was 4.2 years. Children who received GO showed modest elevation of transaminase and bilirubin without signs of veno-occlusive disease. Severe...

  19. Prognostic factors in patients with advanced cholangiocarcinoma:Role of surgery,chemotherapy and body mass index

    Institute of Scientific and Technical Information of China (English)

    Mirna H Farhat; Ali I Shamseddine; Ayman N Tawil; Ghina Berjawi; Charif Sidani; Wael Shamseddeen; Kassem A Barada

    2008-01-01

    AIM:To study the factors that may affect survival of cholangiocarcinoma in Lebanon.METHODS:A retrospective review of the medical records of 55 patients diagnosed with cholangiocarcinoma at the American University of Beirut between 1990 and 2005 was conducted.Univariate and multivariate analyses were performed to determine the impact of surgery,chemotherapy,body mass index,bilirubin level and other factors on survival.RESULTS:The median survival of all patients was 8.57 mo (0.03-105.2).Univariate analysis showed that low bilirubin level (<10 mg/dL),radical surgery and chemotherapy administration were significantly associated with better survival (P = 0.012,0.038 and 0.038,respectively).In subgroup analysis on patients who had no surgery,chemotherapy administration prolonged median survival significantly (17.0 mo vs 3.5 too,P = 0.001).Multivariate analysis identified only low bilirubin level < 10 mg/dL and chemotherapy administration as independent predictors associated with better survival (P < 0.05).CONCLUSION:Our data show that palliative and postoperative chemotherapy as well as a bilirubin level < 10 mg/dL are independent predictors of a significant increase in survival in patients with cholangiocarcinoma.

  20. N-acetyl cysteine therapy in acute viral hepatitis

    Institute of Scientific and Technical Information of China (English)

    Huseyin Gunduz; Oguz Karabay; Ali Tamer; Resat Ozaras; Ali Mert; Omer Fehmi Tabak

    2003-01-01

    AIM: To investigate the effect of N-acetyl cysteine (NAC)on acute viral hepatitis (AVH).METHODS: We administered 200 mg oral NAC three times daily (600 mg/day) to the study group and placebo capsules to the control group. All patients were hospitalized and diagnosed as AVH. Blood total and direct bilirubin, ALT, AST,alkaline phosphatese, albumin and globulin levels of each patient were measured twice weekly until total bilirubin level dropped under 2 mg/dl, ALT level under 100 U/L, follow up was continued and then the patients were discharged.RESULTS: A total of 41(13 female and 28 male) AVH patients were included in our study. The period for normalization of ALT and total bilirubin in the study group was 19.7±6.9 days and 13.7±8.5 days respectively. In the control group it was 20.4±6.5 days and 16.9±7.8 days respectively (P>0.05).CONCLUSION: NAC administration effected neither the time necessary for normalization of ALT and total bilirubin values nor duration of hospitalization, so we could not suggest NAC for the treatment of icteric AVH cases. However, our results have shown that this drug is not harmful to patients with AVH.

  1. Impaired CD163-mediated hemoglobin-scavenging and severe toxic symptoms in patients treated with gemtuzumab ozogamicin

    DEFF Research Database (Denmark)

    Maniecki, M.B.; Hasle, H.; Friis-Hansen, L.;

    2008-01-01

    , and low bilirubin after septicemia-induced intravascular hemolysis indicated abrogated clearance of haptoglobin-hemoglobin complexes. This was further supported by low levels of plasma soluble CD163 and a concordant low number of CD163-expressing monocytes. We show that CD163 positive monocytes...

  2. Clinical study of percutaneous transhepatic balloon dilation: a novel procedure for common bileduct stone

    Institute of Scientific and Technical Information of China (English)

    李玉亮

    2014-01-01

    Objective To investigate the safety and efficacy of percutaneous transhepatic balloon dilation for the removal of common bile duct stone.Methods Sixty-eight cases with common bile duct stone treated with percutaneous transhepatic balloon dilation in our department from July2008 to April 20l1 were analyzed retrospectively.Record CA19-9,total bilirubin,

  3. [Refractometric measurement of total serum protein, comparison of refractometry and biuret test (author's transl)].

    Science.gov (United States)

    Liappis, N; Jäkel, A

    1978-07-01

    Study on the Refractometric Determination of Total Protein in Serum, Comparison of the Refractometric and Biuret Method. Total protein concentration in serum was determined by the aid of the Abbé-refractometer and the biuret method. Both methods showed a good precision and accuracy. The investigation was carried out in 241 sera with normal bilirubin (up to 1 mg/100 ml), cholesterol (up to 200 mg/100 ml) and urea (up to 23,0 mg/100 ml) concentration, in 43 sera with increased (10,6-26,6 mg/100 ml) bilirubin concentration, in 129 sera with increased (200-520 mg/100 ml) cholesterol concentration and in 43 sera with increased (23,0-155,3 mg/100 ml) urea concentration. The comparison of the refractometric values with the values obtained by the biuret method in the 241 sera with normal bilirubin, cholesterol and urea concentration (correlation coefficient = 0,971) showed a close correlation and in the 43 sera with increased bilirubin concentration (correlation coefficient = 0,958) an acceptable correlation. However no close correlations were observed in the 129 sera with increased cholesterol concentration and in the 43 sera with increased urea concentration. The correlation lines diverged proportional with the increase of cholesterol and urea concentration from the expected correlation lines.

  4. 75 FR 14602 - Guidance for Industry on Drug-Induced Liver Injury: Premarketing Clinical Evaluation; Opening of...

    Science.gov (United States)

    2010-03-26

    ... published guidance document. The purpose of the conference is to consider the effect of the recommendations.... It discussed methods of detecting DILI by periodic tests of serum enzyme activities and bilirubin concentration elevations, and how those laboratory tests might change over time, along with symptoms...

  5. Impact of fatty acids on human UDP-glucuronosyltransferase 1A1 activity and its expression in neonatal hyperbilirubinemia.

    Science.gov (United States)

    Shibuya, Ayako; Itoh, Tomoo; Tukey, Robert H; Fujiwara, Ryoichi

    2013-10-09

    While breast milk has been known as a cause of neonatal hyperbilirubinemia, the underlying mechanism of breast milk-induced jaundice has not been clarified. Here, the impact of fatty acids on human UDP-glucuronosyltransferase (UGT) 1A1--the sole enzyme that can metabolize bilirubin--were examined. Oleic acid, linoleic acid, and docosahexaenoic acid (DHA) strongly inhibited UGT1A1 activity. Forty-eight hours after a treatment with a lower concentration of DHA (10 mg/kg), total bilirubin significantly increased in neonatal hUGT1 mice, which are human neonatal jaundice models. In contrast, treatments with higher concentrations of fatty acids (0.1-10 g/kg) resulted in a decrease in serum bilirubin in hUGT1 mice. It was further demonstrated that the treatment with higher concentrations of fatty acids induced UGT1A1, possibly by activation of peroxisome proliferator-activated receptors. Our data indicates that activation of peroxisome proliferator-activated receptors would increase UGT1A1 expression, resulting in reduction of serum bilirubin levels in human infants.

  6. Effective oral treatment of unconjugated hyperbilirubinemia in Gunn rats

    NARCIS (Netherlands)

    Hafkamp, AM; Havinga, R; Sinaasappel, M; Verkade, HJ

    2005-01-01

    We sought to develop an oral treatment for unconjugated hyperbilirubinemia. In the Gunn rat model of unconjugated hyperbilirubinemia, dietary supplementation with the lipase inhibitor orlistat (Or1) or with calcium phosphate (Cap) decreases plasma unconjugated bilirubin (UCB) levels. We determined w

  7. [Extreme hyperbilirubinemia associated with spherocytosis and choledocholithiasis].

    Science.gov (United States)

    Peralta-Zagal, J A; Hernández-Estrada, M

    1990-01-01

    Spherocytosis conditions the severe destruction of red blood cells. The spleen plays an active and fundamental role in this destruction since it is responsible for the fragility and lability of the red blood cell to the splenic medium. The clinical manifestations are: anemia, jaundice, splenomegaly, and hemolytic and aplastic crisis associated to viral infections. Choledocholithiasis is a manifestation of the disease which can be seen at an early stage, even in less severe cases. Choledocholithiasis is rare and this may be due to immediate surgical intervention. The levels of indirect bilirubin may vary but usually do not exceed 10 mg%, on the rising of direct bilirubin may cause suspicion of liver obstruction or damage. A case of a ten year old boy is reported who was found to have spherocytosis, with severe manifestations, who later developed cholelithiasis and total obstruction of the choledocho and in who the most important clinical manifestation was jaundice due to the increase of total bilirubin to 89 mg%, doubly checked. Both a splenectomy and a cholecystectomy were performed. Subsequent checkups were normal. These levels of bilirubin have not been previously reported. Hyperbilirubinemia; spherocytosis; choledocholithiasis.

  8. Prognostic variables in patients with cirrhosis and oesophageal varices without prior bleeding

    DEFF Research Database (Denmark)

    Møller, Søren; Bendtsen, Flemming; Christensen, E

    1994-01-01

    in the study and followed up after an average observation period of 446 days (range: 5-1211 days). A total of 55 clinical, biochemical, haemodynamic, and endoscopic variables were classified as systemic haemodynamic, portal haemodynamic, or metabolic. Using univariate analysis, the following variables showed...... serum bilirubin (p variables as described earlier...

  9. Electrochemical reduction of the biliverdin-serum albumin complex as monitored by absorption and circular dichroism spectroscopy.

    Science.gov (United States)

    Claret, J; Ibars, O; Lang, K; Trull, F R; Lightner, D A

    1995-02-23

    The cathodic reduction at the mercury electrode of a biliverdin IX alpha-serum albumin complex at physiological pH in an aqueous buffer containing percentages of DMSO ranging from 4% to 20% is studied by cyclic voltametry and controlled potential coulometry. The progression of pigment disappearance and the (stereochemical) nature of the product are monitored by chromatography, UV-visible absorption and circular dichroism spectroscopy. Upon reduction, albumin-bound biliverdin IX alpha, with a slight preference for the P-helicity, affords the corresponding bound bilirubin IX alpha -with an M-chirality conformation. The complex is reduced at -0.64 V (vs. SCE; 8% DMSO), only a little shifted compared to reduction of free biliverdin IX alpha under the same conditions. In contrast, an analogous bilirubin IX alpha-serum albumin complex is essentially inert towards cathodic reduction under conditions where free bilirubin IX alpha is reduced, indicating a better shielding by the protein of the bilirubin IX alpha molecule from the electrode surface. The presence of relative position (as in the biliverdins IX alpha and XIII alpha) or absence (as in mesobiliverdin IX alpha) of vinyl groups in the pigment does not have a significant effect upon its electroreduction behaviour, indicating that the process is not sensitive to the subtle differences imposed by vinyl groups upon the structure of the corresponding biliverdin-albumin complexes.

  10. Further investigations on the macromolecular complex in human bile

    NARCIS (Netherlands)

    Verschure, J.C.M.; Wael, J. de; Mijnlieff, P.F.

    1956-01-01

    The formation of complexes in human bile was further studied by the preparation of various synthetic complexes and extracts. These were compared for a number of properties with the natural complex of human gall bladder bile. It appeared that protein is probably and bilirubin quite definitely a const

  11. Impaired cholecystokinin-induced gallbladder emptying incriminated in spontaneous "black" pigment gallstone formation in germfree Swiss Webster mice.

    Science.gov (United States)

    Woods, Stephanie E; Leonard, Monika R; Hayden, Joshua A; Brophy, Megan Brunjes; Bernert, Kara R; Lavoie, Brigitte; Muthupalani, Sureshkumar; Whary, Mark T; Mawe, Gary M; Nolan, Elizabeth M; Carey, Martin C; Fox, James G

    2015-02-15

    "Black" pigment gallstones form in sterile gallbladder bile in the presence of excess bilirubin conjugates ("hyperbilirubinbilia") from ineffective erythropoiesis, hemolysis, or induced enterohepatic cycling (EHC) of unconjugated bilirubin. Impaired gallbladder motility is a less well-studied risk factor. We evaluated the spontaneous occurrence of gallstones in adult germfree (GF) and conventionally housed specific pathogen-free (SPF) Swiss Webster (SW) mice. GF SW mice were more likely to have gallstones than SPF SW mice, with 75% and 23% prevalence, respectively. In GF SW mice, gallstones were observed predominately in heavier, older females. Gallbladders of GF SW mice were markedly enlarged, contained sterile black gallstones composed of calcium bilirubinate and cholecystokinin (CCK), gallbladders of fasted GF SW mice showed impaired emptying (females: 29%; males: 1% emptying), whereas SPF SW females and males emptied 89% and 53% of volume, respectively. Bilirubin secretion rates of GF SW mice were not greater than SPF SW mice, repudiating an induced EHC. Gallstones likely developed in GF SW mice because of gallbladder hypomotility, enabled by features of GF physiology, including decreased intestinal CCK concentration and delayed intestinal transit, as well as an apparent genetic predisposition of the SW stock. GF SW mice may provide a valuable model to study gallbladder stasis as a cause of black pigment gallstones.

  12. Assessment of resin perfusion in hepatic failure in vitro and in vivo

    Institute of Scientific and Technical Information of China (English)

    Ying-Jie Wang; Ze-Wen Wang; Bing-Wei Luo; Hong-Ling Liu; Hong-Wei Wen

    2004-01-01

    AIM: To observe the adsorbent effect of resin on endotoxin,cytokine, bilirubin in plasma of patients with hepatic failure and to determine the resin perfusion as an artificial liver support system in the treatment of hepatic failure.METHODS: One thousand milliliters of discarded plasma was collected from each of 6 severe hepatitis patients treated with plasma exchange. The plasma was passed through a resin perfusion equipment for 1-2 h via extracorporeal circulation, and then absorbent indicators of transaminase,bilirubin, blood ammonia, endotoxin and cytokines were examined. In the meantime, study of in vivo resin plasma perfusion was performed on 7 severe hepatitis patients to compare the changes of endotoxin and cytokines in blood before and after perfusion.RESULTS: The levels of total bilirubin, endotoxin, interleukin 1β and TNF-α in plasma were significantly decreased after in vitro resin plasma perfusion. The levels of interleukin 1β,TNF-α and endotoxin in blood were also evidently declined after in vivo resin plasma perfusion. Nevertheless, no obvious changes in IL-6, creatinine (Cr) and urea nitrogen (UN), blood ammonia and electrolytes were found bothin vitroandin vivo.CONCLUSION: Bilirubin, endotoxin and cytokines in plasma of patients with hepatic failure can be effectively adsorbed by resinin vitro. Most cytokines and endotoxin in plasma can also be effectively removed by resinin vivo. It demonstrates that resin perfusion may have good treatment efficacy on hepatic failure and can be expected to slow down the progression of hepatic failure.

  13. Down-regulation of OATP1B proteins correlates with hyperbilirubinemia in advanced cholestasis

    NARCIS (Netherlands)

    Sticova, E.; Lodererova, A.; Steeg, E. van de; Frankova, S.; Kollar, M.; Lanska, V.; Kotalova, R.; Dedic, T.; Schinkel, A.H.; Jirsa, M.

    2015-01-01

    Aim: Organic anion-transporting polypeptides OATP1B1 and OATP1B3 are sinusoidal membrane transporters mediating liver uptake of a wide range of substrates including conjugated and unconjugated bilirubin, xenobiotics and drugs. Absence of OATP1Bs in the liver causes Rotor syndrome. Our aim was to cor

  14. One Year Oral Toxicity Study of WR238605 Succinate in Dogs. Volume 2

    Science.gov (United States)

    1997-07-18

    219 SEX: MALE Animal ID RBC HGB 10*6/IJITIA3 g/dL HCT HCV MCH MCHC % fL...PRTL219 Urinalvsis Qualitative Bilirubin Nitrite Glucose pH Ketones Protein Occult Blood Urobilinogen Leukocytes Color Specific Gravity

  15. Conformational changes in human serum albumin studied by fluorescence and absorption spectroscopy. Distance measurements as a function of pH and fatty acids

    DEFF Research Database (Denmark)

    Honoré, B; Pedersen, A O

    1989-01-01

    pH- and fatty acid-induced conformational changes in human serum albumin were investigated by fluorescence-energy transfer, determining the distance between Trp-214 and bound bilirubin at 25 degrees C. This distance changes significantly with the pH, being 2.52 +/- 0.01 nm at pH 6, 2.31 +/- 0.04 ...

  16. Cycling parenteral nutrition in a neonatal surgical patient: An argument for increased utilization

    Directory of Open Access Journals (Sweden)

    Katherine W. Gonzalez

    2017-01-01

    Herein, we describe a successful case of cycled TPN in a neonate with complicated gastroschisis and short bowel syndrome. Within three weeks the patient had a normalized direct bilirubin and did not require pharmacologic intervention. There were two episodes of asymptomatic hypoglycemia associated with intestinal obstruction.

  17. Oral treatment of unconjugated hyperbilirubinemia

    NARCIS (Netherlands)

    Hafkamp, Anja Maria

    2006-01-01

    De ziekte van Crigler-Najjar is een ernstige, weinig voorkomende vorm van geelzucht. Kenmerk van de ziekte is ophoping in het lichaam van ongeconjugeerd bilirubine (UCB), een afbraakproduct van rode bloedcellen. Ophoping van UCB kan leiden tot neurologische schade. De gebruikelijke behandeling van d

  18. Quantitative and localized spectroscopy for non-invasive bilirubinometry in neonates

    NARCIS (Netherlands)

    N. Bosschaart

    2012-01-01

    Couveusekinderen lopen een verhoogd risico op geelzucht, wat kan leiden tot hersenschade. Kinderen met geelzucht krijgen meerdere hielprikken per dag om de bilirubinewaarde in het bloed te bepalen. Bilirubine is het afbraakproduct van hemoglobine, dat kan zorgen voor een gelige huidskleur. Een mogel

  19. [The general practitioner and abnormal liver function tests].

    Science.gov (United States)

    Hallez, R

    1997-09-01

    In case of abnormal liver function tests, it's necessary to distinguish different situations, starting from this first data. We will successively consider: the high and moderate acute increases of aminotransferase, the chronic increases of aminotransferase, the isolated cholestase picture and the isolated increases of gamma GT or of bilirubine. We will finish with a partial survey about drug-induced liver diseases.

  20. Bereiding en certificatie van primaire bilirubinestandaarden

    NARCIS (Netherlands)

    Wikkeling RH; Dreumel HJ van; Phielix-Strubbe CJ; Koedam JC

    1988-01-01

    Dit rapport beschrijft de bereiding en de certificatie van vijf partijen gevriesdroogde bilirubinestandaarden. De partijen bestonden uit nauwkeurig afgewogen hoeveelheden bilirubine, opgelost in humaan serumalbumine oplossingen en hadden een concentratie van resp. 0, 25, 75, 100 en 300 mumol/l (

  1. Novel treatment strategies for unconjugated hyperbilirubinemia

    NARCIS (Netherlands)

    Cuperus, Frans Jan Christiaan

    2011-01-01

    Ongeconjugeerde hyperbilirubinemie (de ophoping van bilirubine in het bloed) komt vooral voor bij mensen met de ziekte van Crigler-Najjar en bij sommige pasgeborenen. De standaardbehandeling voor ongeconjugeerde hyperbilirubinemie bestaat uit fototherapie; de bestraling van de huid met blauw licht.

  2. National Institutes of Health Consensus Development Project on Criteria for Clinical Trials in Chronic Graft-versus-Host Disease: I. Diagnosis and Staging Working Group Report

    Science.gov (United States)

    2005-09-09

    keratoconjunctivitis sicca GI TRACT No symptoms Symptoms such as dysphagia , anorexia, nausea, vomiting, abdominal pain or diarrhea without...requires nutritional supplement for most calorie needs OR esophageal dilation LIVER Normal LFT Elevated Bilirubin, AP*, AST or ALT ɚ x ULN

  3. Heme degradation and human disease: diversity is the soul of life.

    Science.gov (United States)

    Shibahara, Shigeki; Kitamuro, Tomomi; Takahashi, Kazuhiro

    2002-08-01

    We all depend on molecular oxygen and heme for our life, as evident from the pigments in blood and daily wastes. About 80% of serum bilirubin is derived from hemoglobin of senescent erythrocytes, which have finished their mission of 120 days and have been phagocytized by macrophages in the reticuloendothelial system. Here we present an overview of the heme degradation processes and relevant disorders by focusing on heme oxygenase-1 (HO-1), a key enzyme in heme catabolism. HO-1 cleaves the porphyrin macrocycle of heme at the expense of molecular oxygen to release a linear tetrapyrrole biliverdin, carbon monoxide, and ferrous iron; biliverdin is rapidly reduced to bilirubin. Bilirubin is transported to the liver (hepatocytes), conjugated with glucuronic acid by bilirubin UDP-glucuronosyltransferase, and excreted into bile. Genetic diversity, a strategy in the host defense, is seen in the human ho-1 and UDP-glucuronosyltransferase genes. Moreover, striking interspecies variations are noted in the regulation of HO-1 expression by hypoxia, heat shock, or interferon-gamma, each of which mainly represses HO-1 expression in human cells. Implications of such a variety are discussed in relevance to the pathogenesis of severe malaria caused by Plasmodium falciparum, the most ancient foe of humans.

  4. Experimental Characterization of Near-Infrared Laser Energy Absorption, Scattering, and Transmittance in Biological Tissue

    Science.gov (United States)

    2007-03-01

    the heme pigment of hemoglobin, myoglobin, and bilirubin [12]. The main tissue chromophores in the infrared region are lipofuscine, xantophyll, melanin...water and tissue chromophores that include certain cellular pigments . The natural chromophores present include the biological pigments — specifically

  5. Toxicological evaluation of the hydro-alcohol extract of the dry leaves of Peumus boldus and boldine in rats.

    Science.gov (United States)

    Almeida, E R; Melo, A M; Xavier, H

    2000-03-01

    The hydro-alcohol extract of the dry leaves of Peumus boldus and boldine, showed abortive and teratogenic action and changes in the blood levels of bilirubin, cholesterol, glucose, alanine aminotransferase (ALT), aspartate aminotransferase (AST) and urea in rats. The long term administration of the extract and boldine did not cause histological modification during a period of 90 days.

  6. Refractory obstructive jaundice in a child affected with thalassodrepanocytosis: a new endoscopic approach

    Directory of Open Access Journals (Sweden)

    Barresi Luca

    2010-10-01

    Full Text Available Abstract Background Liver involvement, including elevated direct-reacting bilirubin levels, is common in patients with sickle cell disease. Fifty to seventy percent of sickle cell patients have pigmented gallstones due to precipitation of unconjugated bilirubin, and cholelithiasis or choledocholithiasis are common complications. The highest prevalence of these complications occurs in patients with Gilbert's syndrome because of the combined effect of increased bilirubin production and reduced bilirubin-diphosphate-glucuronosyltransferase enzyme activity. Cholelithiasis is also a common complication in patients with thalassemia. Endoscopic removal of choledochal stones does not always resolve the clinical picture, as in cases of dysfunction of the Vater's papilla, increased bile density due to persistently impaired bile flow or distortion of the choledocus due to dilatation, or inflammation secondary to gallstone. Case presentation We report here a case of severe and persistent obstructive jaundice in a child affected with thalassodrepanocytosis and Gilbert's syndrome, previously, and unsuccessfully, treated with endoscopic removal of choledochal stones. Deep and thorough biliary washing, and stenting with a new removable polytetrafluoroethylene (PTFE-covered flared-type stent led to complete resolution of the obstructive jaundice. Conclusions This report shows that an aggressive endoscopic approach in this select category of patients can help resolve the severe complication of hemolytic anemia, thus avoiding surgery.

  7. The effect of clofibrate and phototherapy on physiological jaundice in term newborns

    Directory of Open Access Journals (Sweden)

    Amir Hosein Hashemian

    2011-09-01

    Full Text Available Background: Clofibrate is an effective anti lipid agent that induces glucuronyltransferase could increase bilirubin conjugation. The aim of this study was to evaluate effect of clofibrate on neonatal physiologic jaundice.Methods: Randomized clinical trial sampling method used and 60 healthy term neonates which were admitted in Imam Reza Hospital of Kermanshah-Iran because of indirect hyperbilirubinemia enrolled into the study. 30 neonates (case group were treated with single oral dose of clofibrate (100/mg plus phototherapy and 30 neonates (control group received only phototherapy. Serum total and direct bilirubin levels were measured at admission, 12 hours later, and then every 24 hours until 96 hours. Results: There were no significant difference between two groups regarding to gender, age, weight and total serum bilirubin level at the admission. Mean values for total bilirubin of serum in case group 12, 24 and 48 hours after admission were significantly lower than control group (P<0.001. The mean of needed time for phototherapy in case group was significantly less than the control group (P<0.00l.Conclusion: It seems that clofibrate plus phototherapy is effective for treatment of neonatal physiologic jaundice in healthy term newborns, although further studies are necessary for evaluation of clofibrate safety as a routine treatments.

  8. A Study to Determine Product Costs for Chemistry Laboratory Tests at Darnall Army Community Hospital

    Science.gov (United States)

    1986-06-01

    purposes, many test procedures whose time values were identical were grouped together, e.g. all of the Dupont (trademark) automated chemistry analyzer tests...department for use in apportioning indirect expenses. 15 Figure 5. Dupont automated chemistry analyzer tests calcium bilirubin magnesium salicylic acid amylase...case mix cost accounting process ................ 9 4. Workcenter organization .................................... 14 5. DuPont automated chemistry

  9. Comparison of nutritional parameters after abo incompatible living donor renal transplantation

    Directory of Open Access Journals (Sweden)

    Joon Seok Oh

    2012-06-01

    By the end of the first year, serum hemoglobin, calcium, albumin, HDL, bilirubin, AST, ALT were increased statistically. But serum phosphate, globulin were decreased statistically. In conclusion, successful ABO incompatible living donor kidney transplantation would restore a normal nutritional status even though the patients were performed plasmapheresis during the pre-transplant period.

  10. Kernicterus is vermijdbaar, maar komt nog steeds voor

    NARCIS (Netherlands)

    Bousema, Sara; Govaert, Paul; Dudink, Jeroen; Steegers, Eric A P; Reiss, Irwin K M; de Jonge, Rogier C J

    2015-01-01

    Kernicterus is a severe neurological condition, caused by bilirubin-induced damage in the basal ganglia. The neurological outcome is often poor. In the past decades there seems to have been an increase in the number of reported cases of kernicterus. In order to raise awareness of this condition, we

  11. Jaundice

    Science.gov (United States)

    ... PA: Elsevier Mosby; 2014:chap 28. Read More Autoimmune hepatitis Bile Bile duct stricture Biliary atresia Bilirubin blood test Cholestasis Delta agent (Hepatitis D) Dubin-Johnson syndrome Gallstones Gilbert disease Hemolytic anemia Hepatitis A Hepatitis B Hepatitis C Malaria Newborn ...

  12. Neonates and Infants At Risk for Hearing and Speech-Language Disorders.

    Science.gov (United States)

    Clark, David A.

    1989-01-01

    High-risk infants may exhibit hearing impairments which can subsequently impede speech/language development. Discussed are prenatal risk factors, including environmental toxins and infections; and perinatal factors such as prematurity, asphyxia, intracranial hemorrhage, bilirubin, ototoxic drugs, and environmental noise. A table summarizes factors…

  13. Urine testing for diabetic analysis

    Directory of Open Access Journals (Sweden)

    Janet Marsden

    2016-04-01

    Full Text Available Urine testing is relatively cheap and easy to do. Urine testing can be used to check for blood in the urine, to check for infection (by detecting the presence of white blood cells or protein and can show up other systemic problems such as liver problems (by showing abnormal bilirubin levels. Urine testing can also detect ketones in the urine.

  14. Clinical effect by exchange transfusion in the treatment of hemolytic disease of the newborn%换血方法治疗新生儿溶血病的临床疗效

    Institute of Scientific and Technical Information of China (English)

    赵鲁强

    2016-01-01

    目的:探讨换血方法治疗新生儿溶血病(HDN)的临床效果。方法120例HDN患儿进行换血治疗,分别检测患儿在换血前后的血清总胆红素、直接胆红素、间接胆红素及Na+、Cl-、Ca2+、K+的水平变化。结果120例患儿换血后血清总胆红素、直接胆红素和间接胆红素水平与换血前比较均明显降低,差异均有统计学意义(P<0.05)。120例患儿与换血前比较,换血后Na+、Cl-、Ca2+水平均显著升高, K+显著下降,差异均有统计学意义(P<0.05)。结论换血方法可以降低HDN患儿胆红素水平,纠正电解质紊乱,临床值得推广应用。%ObjectiveTo investigate clinical effect by exchange transfusion in the treatment of hemolytic disease of the newborn (HDN).MethodsA total of 120 infants with HDN received exchange transfusion. Their changes of serum total bilirubin, direct bilirubin, indirect bilirubin, level changes of Na+, Cl-, Ca2+, and K+ were all detected before and after exchange transfusion.ResultsThe differences of serum total bilirubin, direct bilirubin, and indirect bilirubin, which were abviously decreased, before and after exchange transfusion had statistical significance in 120 cases (P<0.05). Comparing with those before exchange transfusion, all the 120 cases had much higher Na+, Cl-, Ca2+ levels after exchange transfusion, and K+ level was obviously reduced after exchange transfusion, and the differences had statistical significance (P<0.05).ConclusionExchange transfusion can decrease bilirubin level and correct electrolyte disturbance in HDN infants. This method is worth clinical promotion and application.

  15. Hepatoprotective activity of the methanolic extract of Fagonia indica Burm in carbon tetra chloride induced hepatotoxicity in albino rats

    Institute of Scientific and Technical Information of China (English)

    I.M. Bagban; S.P. Roy; A.Chaudhary; S. K. Das; K.J. Gohil; K.K. Bhandari

    2012-01-01

    Objective: To investigate hepatoprotective activity of the methanolic extract of Fagonia indica Burm. on CCl4 induced hepatotoxicity in albino rats. Methods: Animals in Group 1 served as vehicle control, Group 2 served as hepatotoxin (CCl4 2ml/kg, s.c) treated group, Group 3 served as standard (Silymarin 50mg/kg, p.o.) treated group. Group4 and 5 served as methanolic extract of Fagonia indica (MEFI) in different doses (200 mg/kg and 400 mg/kg b.w., p.o).The degree of protection was determined by measuring levels of biochemical marker like SGOT, SGPT, ALP, Bilirubin (Total & Direct) and Cholesterol. The histopathological studies also show the hepatic protection of the test extracts. Results: The levels of the biochemical parameters such as SGPT, SGOT, ALP, Total bilirubin, Direct bilirubin and Cholesterol were significantly increased in CCl4 treated rats when compared with the normal group (P<0.05), but the MEFI (400 mg/kg, bw) treated rats showed maximum reduction of SGOT (114.83±1.51), SGPT (164.33±1.25), ALP (154.83±1.53), Total bilirubin (1.55±0.01), Direct bilirubin (0.65±0.009) and Cholesterol (193.00±1.06) in a significant manner. Histopathological studies also reveal the hepatoprotection property of MEFI in a dose dependent manner. Conclusions: These results suggest that MEFI in different doses showed significant hepatoprotective activity against CCl4 induced hepatotoxicity and this might be due to the presence of flavonoids and tannins. Further research is sought to explore the exact mechanism of action and phytoconstituents responsible for the pharmacological response.

  16. Disruption of the ugt1 locus in mice resembles human Crigler-Najjar type I disease.

    Science.gov (United States)

    Nguyen, Nghia; Bonzo, Jessica A; Chen, Shujuan; Chouinard, Sarah; Kelner, Michael J; Hardiman, Gary; Bélanger, Alain; Tukey, Robert H

    2008-03-21

    The 9 UDP-glucuronosyltranferases (UGTs) encoded by the UGT1 locus in humans are key enzymes in the metabolism of most drugs as well as endogenous substances such as bile acids, fatty acids, steroids, hormones, neurotransmitters, and bilirubin. Severe unconjugated hyperbilirubinemia in humans that suffer from Crigler-Najjar type I disease results from lesions in the UGT1A1 gene and is often fatal. To examine the physiological importance of the Ugt1 locus in mice, this locus was rendered non-functional by interrupting exon 4 to create Ugt1(-/-) mice. Because UGT1A1 in humans is responsible for 100% of the conjugated bilirubin, it followed that newborn Ugt1(-/-) mice developed serum levels of unconjugated bilirubin that were 40-60 times higher than Ugt1(+/-) or wild-type mice. The result of extreme unconjugated bilirubin in Ugt1(-/-) mice, comparable to the induced levels noted in patients with Crigler-Najjar type 1 disease, is fatal in neonatal Ugt1(-/-) mice within 2 weeks following birth. The extreme jaundice is present as a phenotype in skin color after 8 h. Neonatal Ugt1(-/-) mice exhibit no detectable UGT1A-specific RNA, which corresponds to a complete absence of UGT1A proteins in liver microsomes. Conserved glucuronidation activity attributed to the Ugt1 locus can be defined in Ugt1(-/-) mice, because UGT2-dependent glucuronidation activity is unaffected. Remarkably, the loss of UGT1A functionality in liver results in significant alterations in cellular metabolism as investigated through changes in gene expression. Thus, the loss of UGT1A function in Ugt1(-/-) mice leads to a metabolic syndrome that can serve as a model to further investigate the toxicities associated with unconjugated bilirubin and the impact of this disease in humans.

  17. Progress in the study on the composition and formation mechanism of gallstone

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    Our serial studies from 1970s on chemical composition, structure determination and formation mechanism of gallstones were reviewed. The chemical component investigation of brown-pigment gallstone demonstrated that it consists of macromolecules such as proteins, glycoproteins, polysaccharides, bilirubin polymers and pigment polymers, and biomolecules such as cholesterol, bile salts, calcium salts of carbonate, phosphate, fatty acids and bilirubinate as well as various metal ions. The binding of metal ions with bile salts and bilirubin plays important roles in gallstone formation, i.e., calcium bilirubinate complex is the major constitute of brown-pigment gallstones, and copper bilirubinate complex is critical in the black color appearance of black-pigment gallstone. The cross section of many gallstones exhibits a concentric ring structure composed of various small particles with a fractal character. This is nonlinear phenomenon in gallstone formation. A typical model system of metal ions-deoxycholate (or cholate)-gel was chosen to mimic an in vitro pattern formation system. The experimental results suggested that a nonlinear scientific concept should be considered in understanding gallstone formation. Minor changes in the chemical composition and/or the microenvironment may lead to very different precipitate patterns with a variety of shapes, colors, appearances, and structures. A new model was suggested that periodical templets of periodical and fractal patterns were formed in the initial stage, then the spatio-temporal patterns grew gradually on it. Furthermore, the interaction between divalent metal ions and bile salts in vitro was investigated, and the results indicated that non-stoichiometric M(DC)2-NaDC mixed complexes with mixed micelles structure can be formed in physiological condition.

  18. Antioxidant status in neonatal jaundice before and after phototherapy

    Directory of Open Access Journals (Sweden)

    S Ayyappan

    2015-01-01

    Full Text Available Background: Neonatal jaundice refers to yellow coloration of the skin and the sclera (whites of the eyes of newborn babies that result from the accumulation of bilirubin in the skin and mucous membranes. Because bilirubin is potentially toxic to the central nervous system. Genetic disorders of bilirubin conjugation, particularly the common Gilbert′s syndrome, can also contribute to neonatal hyperbilirubinemia. Objective: The aim of this study was to evaluate the lipid per-oxidation and antioxidant enzyme activities in patients with neonatal jaundice before and after phototherapy. Materials and Methods: The study includes 50 neonatal jaundice patients with average age 2-15 days. All patients of neonatal jaundice receiving phototherapy except feeding, cleaning. Subjects selected were from the patients attending Pediatrics Department. Plasma malondialdehyde (MDA, erythrocyte glutathione peroxidase (GPX, superoxide dismutase and catalase (CAT to monitor the bilirubin level. Results: The results show increased levels of bilirubin compared with controls (P < 0.001 shows the level of plasma MDA in control, before and after phototherapy. Represents the level of GPX was significantly increased in after the phototherapy group when compared with before phototherapy and control SPSS soft ware: (P < 0.001. Shows the reduced glutathione (GSH level in plasma was significantly decreased in the after phototherapy group when compared with before phototherapy and control (P < 0.001. And finally with ascorbic acid and CAT. Conclusion: It is evident from the study that increased oxidative stress in neonatal jaundice babies leads to decrease in the levels of antioxidants like GSH and ascorbic acid and disturb their metabolism, that weaken their ability to fight the growing stress. Intense oxidative stress and decreased antioxidants may contribute to neural cell death and alter the erythrocytomembrane structure processing in neonatal jaundice.

  19. Total serum bile acid as a potential marker for the diagnosis of cholangiocarcinoma without jaundice.

    Science.gov (United States)

    Sombattheera, Sutthikan; Proungvitaya, Tanakorn; Limpaiboon, Temduang; Wongkham, Sopit; Wongkham, Chaisiri; Luvira, Vor; Proungvitaya, Siriporn

    2015-01-01

    Diagnosis of cholangiocarcinoma (CCA) is difficult when patients do not show jaundice. The aim of this study was to examine the feasibility of using the total serum bile acid (TSBA) level as an aid for the diagnosis of CCA in patients without jaundice. For this purpose, TSBA of the following groups were measured using a Beckman Synchron CX4 clinical chemistry analyzer: 60 cases of CCA with total serum bilirubin ≤2 mg/dL (low total bilirubin group, LTB); 32 cases of CCA with total serum bilirubin >2 mg/dL (high total bilirubin group, HTB); and 115 healthy controls. Liver function parameters such as serum cholesterol, albumin, alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP) were also examined. The results showed that the TSBA of both LTB and HTB groups of the CCA patients were significantly higher than that of the healthy controls. Also, significant correlation was observed between TSBA and total bilirubin levels in the HTB group of CCA patients. However, no such correlation was seen in the LTB group. The cut-off value of TSBA was determined for the LTB group of CCA patients using the receiver operating characteristic curve analysis, and it was 6.05 μmol/L with the sensitivity and specificity of 46.7% and 84.4%, respectively. In addition, the ALP level was correlated well with the TSBA level and ALP in HTB group was significantly higher than that of LTB group. Moreover, the combination of high TSBA and high ALP levels gave higher specificity up to 97.4%. TSBA might be useful for the diagnosis of CCA patients without jaundice.

  20. Prediction of severe neonatal hyperbilirubinemia using cord blood hydrogen peroxide: a prospective study.

    Directory of Open Access Journals (Sweden)

    Hung-Chieh Chou

    Full Text Available BACKGROUND: We hypothesized that cord blood hydrogen peroxide (H2O2 could be utilized to predict the severity of neonatal hyperbilirubinemia. METHODS: We prospectively enrolled term or near-term healthy neonates. Cord blood and capillary blood at three days of age were measured for hydrogen peroxide and bilirubin concentrations. For newborns with hyperbilirubinemia, further blood samples were obtained at five and seven days of age. Newborns were divided into severe or less severe hyperbilirubinemic groups (peak bilirubin ≥17 mg/dL or not. The sensitivity, specificity, and negative predictive values were determined. RESULTS: There were 158 neonates enrolled. The incidence of neonatal hyperbilirubinemia was 30.5% for a concentration ≥15 mg/dl. The rising patterns were similar among bilirubin concentrations and hydrogen peroxide levels during the first few days of life. There was a strong positive correlation between bilirubin concentrations and hydrogen peroxide levels after correlation analysis. The rate of severe hyperbilirubinemia was 13.3%. It revealed that a cord blood hydrogen peroxide signal level of 2500 counts/10 seconds was an appropriate cut-off for predicting severe hyperbilirubinemia. Sensitivity and the negative predictive value were 76.2% and 93.3%, respectively. CONCLUSIONS: Our findings confirm that hydrogen peroxide levels and bilirubin concentrations in cord and neonatal blood are closely related. A cord blood hydrogen peroxide level above 2500 counts/10 seconds associated with a high predictive value for severe hyperbilirubinemia. This method provides information about which neonate should be closely followed after discharge from the nursery.