WorldWideScience

Sample records for biacore spr experiment

  1. SPR/MS

    DEFF Research Database (Denmark)

    Borch, Jonas; Roepstorff, Peter

    2010-01-01

    Surface plasmon resonance is widely used to study binding interactions with proteins, potentially yielding information on kinetics, thermodynamics and active concentrations. However, the technology cannot identify the involved interaction partners. Mass spectrometry, on the other hand, can be used...... for specific identification of proteins in amounts comparable to the levels that can be captured on a Biacore SPR sensorchip. Here we present protocols for capturing, washing and eluting proteins from Biacore instruments as well as for robust sample preparation for sensitive mass spectrometric identification....

  2. Thermodynamic benchmark study using Biacore technology

    NARCIS (Netherlands)

    Navratilova, I.; Papalia, G.A.; Rich, R.L.; Bedinger, D.; Brophy, S.; Condon, B.; Deng, T.; Emerick, A.W.; Guan, H.W.; Hayden, T.; Heutmekers, T.; Hoorelbeke, B.; McCroskey, M.C.; Murphy, M.M.; Nakagawa, T.; Parmeggiani, F.; Xiaochun, Q.; Rebe, S.; Nenad, T.; Tsang, T.; Waddell, M.B.; Zhang, F.F.; Leavitt, S.; Myszka, D.G.

    2007-01-01

    A total of 22 individuals participated in this benchmark study to characterize the thermodynamics of small-molecule inhibitor-enzyme interactions using Biacore instruments. Participants were provided with reagents (the enzyme carbonic anhydrase II, which was immobilized onto the sensor surface, and

  3. SPR salt wall leaching experiments in lab-scale vessel : data report.

    Energy Technology Data Exchange (ETDEWEB)

    Webb, Stephen Walter; O' Hern, Timothy John; Hartenberger, Joel David

    2010-10-01

    During cavern leaching in the Strategic Petroleum Reserve (SPR), injected raw water mixes with resident brine and eventually interacts with the cavern salt walls. This report provides a record of data acquired during a series of experiments designed to measure the leaching rate of salt walls in a labscale simulated cavern, as well as discussion of the data. These results should be of value to validate computational fluid dynamics (CFD) models used to simulate leaching applications. Three experiments were run in the transparent 89-cm (35-inch) ID diameter vessel previously used for several related projects. Diagnostics included tracking the salt wall dissolution rate using ultrasonics, an underwater camera to view pre-installed markers, and pre- and post-test weighing and measuring salt blocks that comprise the walls. In addition, profiles of the local brine/water conductivity and temperature were acquired at three locations by traversing conductivity probes to map out the mixing of injected raw water with the surrounding brine. The data are generally as expected, with stronger dissolution when the salt walls were exposed to water with lower salt saturation, and overall reasonable wall shape profiles. However, there are significant block-to-block variations, even between neighboring salt blocks, so the averaged data are considered more useful for model validation. The remedial leach tests clearly showed that less mixing and longer exposure time to unsaturated water led to higher levels of salt wall dissolution. The data for all three tests showed a dividing line between upper and lower regions, roughly above and below the fresh water injection point, with higher salt wall dissolution in all cases, and stronger (for remedial leach cases) or weaker (for standard leach configuration) concentration gradients above the dividing line.

  4. Investigation of oil injection into brine for the Strategic Petroleum Reserve : hydrodynamics and mixing experiments with SPR liquids.

    Energy Technology Data Exchange (ETDEWEB)

    Castaneda, Jaime N.; Cote, Raymond O.; Torczynski, John Robert; O' Hern, Timothy John

    2004-05-01

    An experimental program was conducted to study a proposed approach for oil reintroduction in the Strategic Petroleum Reserve (SPR). The goal was to assess whether useful oil is rendered unusable through formation of a stable oil-brine emulsion during reintroduction of degassed oil into the brine layer in storage caverns. An earlier report (O'Hern et al., 2003) documented the first stage of the program, in which simulant liquids were used to characterize the buoyant plume that is produced when a jet of crude oil is injected downward into brine. This report documents the final two test series. In the first, the plume hydrodynamics experiments were completed using SPR oil, brine, and sludge. In the second, oil reinjection into brine was run for approximately 6 hours, and sampling of oil, sludge, and brine was performed over the next 3 months so that the long-term effects of oil-sludge mixing could be assessed. For both series, the experiment consisted of a large transparent vessel that is a scale model of the proposed oil-injection process at the SPR. For the plume hydrodynamics experiments, an oil layer was floated on top of a brine layer in the first test series and on top of a sludge layer residing above the brine in the second test series. The oil was injected downward through a tube into the brine at a prescribed depth below the oil-brine or sludge-brine interface. Flow rates were determined by scaling to match the ratio of buoyancy to momentum between the experiment and the SPR. Initially, the momentum of the flow produces a downward jet of oil below the tube end. Subsequently, the oil breaks up into droplets due to shear forces, buoyancy dominates the flow, and a plume of oil droplets rises to the interface. The interface was deflected upward by the impinging oil-brine plume. Videos of this flow were recorded for scaled flow rates that bracket the equivalent pumping rates in an SPR cavern during injection of degassed oil. Image-processing analyses were

  5. Capture-stabilize approach for membrane protein SPR assays.

    Science.gov (United States)

    Chu, Ruiyin; Reczek, David; Brondyk, William

    2014-12-08

    Measuring the binding kinetics of antibodies to intact membrane proteins by surface plasmon resonance has been challenging largely because of the inherent difficulties in capturing membrane proteins on chip surfaces while retaining their native conformation. Here we describe a method in which His-tagged CXCR5, a GPCR, was purified and captured on a Biacore chip surface via the affinity tag. The captured receptor protein was then stabilized on the chip surface by limited cross-linking. The resulting chip surface retained ligand binding activity and was used for monoclonal antibody kinetics assays by a standard Biacore kinetics assay method with a simple low pH regeneration step. We demonstrate the advantages of this whole receptor assay when compared to available peptide-based binding assays. We further extended the application of the capture-stabilize approach to virus-like particles and demonstrated its utility analyzing antibodies against CD52, a GPI-anchored protein, in its native membrane environment. The results are the first demonstration of chemically stabilized chip surfaces for membrane protein SPR assays.

  6. Comparative analyses of a small molecule/enzyme interaction by multiple users of Biacore technology

    NARCIS (Netherlands)

    Cannon, M.J.; Papalia, G.A.; Navratilova, I.; Fisher, R.J.; Roberts, L.R.; Worthy, K.M.; Stephen, A.G.; Marchesini, G.R.; Collins, E.J.; Casper, D.; Qiu, H.; Satpaev, D.; Liparoto, S.F.; Rice, D.A.; Gorshkova, I.; Darling, R.J.; Bennett, D.B.; Sekar, M.; Hommema, E.; Liang, A.M.; Day, E.S.; Inman, J.; Karlicek, S.H.; Ullrich, S.J.; Hodges, D.; Chu, T.; Sullivan, E.; Simpson, J.; Rafique, A.; Luginbühl, B.; Nyholm Westin, S.; Bynum, M.; Cachia, P.; Li, Y.J.; Kao, D.; Neurauter, A.; Wong, M.

    2004-01-01

    To gauge the experimental variability associated with Biacore analysis, 36 different investigators analyzed a small molecule/enzyme interaction under similar conditions. Acetazolamide (222 g/mol) binding to carbonic anhydrase II (CAII; 30,000 Da) was chosen as a model system. Both reagents were stab

  7. SPR 2015. Abstracts

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2015-04-01

    The volume contains the abstracts of the SPR (society for pediatric radiology) 2015 meeting covering the following issues: fetal imaging, muscoskeletal imaging, cardiac imaging, chest imaging, oncologic imaging, tools for process improvement, child abuse, contrast enhanced ultrasound, image gently - update of radiation dose recording/reporting/monitoring - meaningful or useless meaning?, pediatric thoracic imaging, ALARA.

  8. High-resolution characterization of antibody fragment/antigen interactions using Biacore T100.

    Science.gov (United States)

    Papalia, Giuseppe A; Baer, Mark; Luehrsen, Kenneth; Nordin, Helena; Flynn, Peter; Myszka, David G

    2006-12-01

    A Biacore T100 optical biosensor was used to characterize the binding kinetics of a panel of antigen binding fragments (Fabs) directed against the PcrV protein from Pseudomonas aeruginosa. PcrV protein forms part of the type III secretion system complex of this opportunistic pathogen. We demonstrate that the biosensor response data for each Fab collected from three different surface densities of the antigen could be fit globally to a simple 1:1 interaction model. Importantly, we found that the Fabs with the slowest dissociation rate provided the best protection in cell cytotoxicity studies. To further characterize the Fab interactions, binding data were automatically acquired at different temperatures and under different buffer conditions. The comprehensive characterization of these Fabs shows how Biacore T100 can be used to complement protein therapeutic discovery programs from basic research to the selection of therapeutic candidates.

  9. SPR 2014. Abstracts

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2014-05-15

    The proceedings of the SPR 2014 meeting include abstracts on the following topics: Body imaging techniques: practical advice for clinic work; thoracic imaging: focus on the lungs; gastrointestinal imaging: focus on the pancreas and bowel; genitourinary imaging: focus on gonadal radiology; muscoskeletal imaging; focus on oncology; child abuse and nor child abuse: focus on radiography; impact of NMR and CT imaging on management of CHD; education and communication: art and practice in pediatric radiology.

  10. SPR 2017. Abstracts

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2017-05-15

    The conference proceedings SPR 2017 include abstracts on the following issues: gastrointestinal radiography - inflammatory bowel diseases, cardiovascular CTA, general muscoskeletal radiology, muscoskeletal congenital development diseases, general pediatric radiology - chest, muscoskeletal imaging - marrow and infectious disorders, state-of-the-art body MR imaging, practical pediatric sonography, quality and professionalism, CT imaging in congenital heart diseases, radiographic courses, body MT techniques, contrast enhanced ultrasound, machine learning, forensic imaging, the radiation dos conundrum - reconciling imaging, imagining and managing, the practice of radiology, interventional radiology, neuroradiology, PET/MR.

  11. Analysis of human chorionic gonadotropin-monoclonal antibody interaction in BIAcore

    Indian Academy of Sciences (India)

    Banerjee Ashish; Gundlupet Satyanarayana Murthy

    2004-03-01

    Kinetic studies of macromolecular ligand-ligate interaction have generated ample interest since the advent of plasmon resonance based instruments like BIAcore. Most of the studies reported in literature assume a simple 1 : 1 Langmuir binding and complete reversibility of the system. However we observed that in a high affinity antigen-antibody system [human chorionic gonadotropin-monoclonal antibody (hCG-mAb)] dissociation is insignificant and the sensogram data cannot be used to measure the equilibrium and kinetic parameters. At low concentrations of mAb the complete sensogram could be fitted to a single exponential. Interestingly we found that at higher mAb concentrations, the binding data did not conform to a simple bimolecular model. Instead, the data fitted a two-step model, which may be because of surface heterogeneity of affinity sites. In this paper, we report on the global fit of the sensograms. We have developed a method by which a single two-minute sensogram can be used in high affinity systems to measure the association rate constant of the reaction and the functional capacity of the ligand (hCG) immobilized on the chip. We provide a rational explanation for the discrepancies generally observed in most of the BIAcore sensograms.

  12. Comparative analysis of 10 small molecules binding to carbonic anhydrase II by different investigators using Biacore technology.

    Science.gov (United States)

    Papalia, Giuseppe A; Leavitt, Stephanie; Bynum, Maggie A; Katsamba, Phinikoula S; Wilton, Rosemarie; Qiu, Huawei; Steukers, Mieke; Wang, Siming; Bindu, Lakshman; Phogat, Sanjay; Giannetti, Anthony M; Ryan, Thomas E; Pudlak, Victoria A; Matusiewicz, Katarzyna; Michelson, Klaus M; Nowakowski, Agnes; Pham-Baginski, Anh; Brooks, Jonathan; Tieman, Bryan C; Bruce, Barry D; Vaughn, Michael; Baksh, Michael; Cho, Yun Hee; Wit, Mieke De; Smets, Alexandra; Vandersmissen, Johan; Michiels, Lieve; Myszka, David G

    2006-12-01

    In this benchmark study, 26 investigators were asked to characterize the kinetics and affinities of 10 sulfonamide inhibitors binding to the enzyme carbonic anhydrase II using Biacore optical biosensors. A majority of the participants collected data that could be fit to a 1:1 interaction model, but a subset of the data sets obtained from some instruments were of poor quality. The experimental errors in the k(a), k(d), and K(D) parameters determined for each of the compounds averaged 34, 24, and 37%, respectively. As expected, the greatest variation in the reported constants was observed for compounds with exceptionally weak affinity and/or fast association rates. The binding constants determined using the biosensor correlated well with solution-based titration calorimetry measurements. The results of this study provide insight into the challenges, as well as the level of experimental variation, that one would expect to observe when using Biacore technology for small molecule analyses.

  13. Fabrication and characterization of SPR chips with the modified bovine serum albumin

    Science.gov (United States)

    Chen, Xing; Zhang, Lu-lu; Cui, Da-fu

    2016-03-01

    A facile surface plasmon resonance (SPR) chip is developed for small molecule determination and analysis. The SPR chip was prepared based on a self assembling principle, in which the modified bovine serum albumin (BSA) was directly self-assembled onto the bare gold surface. The surface morphology of the chip with the modified BSA was investigated by atomic force microscopy (AFM) and its optical properties were characterized. The surface binding capacity of the bare facile SPR chip with a uniform morphology is 8 times of that of the bare control SPR chip. Based on the experiments of immune reaction between cortisol antibody and cortisol derivative, the sensitivity of the facile SPR chip with the modified BSA is much higher than that of the control SPR chip with the un-modified BSA. The facile SPR chip has been successfully used to detect small molecules. The lowest detection limit is 5 ng/mL with a linear range of 5—100 ng/mL for cortisol analysis. The novel facile SPR chip can also be applied to detect other small molecules.

  14. Språka och lek

    DEFF Research Database (Denmark)

    Bylander, Helle Iben; Thomsen, Inge Benn

    Språka och Lek innehåller mer än 100 språklekar och idéer. Bokens aktiviteter är noggrant beskrivna med tydliga mål och anvisningar, så att den pedagogiska personalen i förskolan kan erbjuda spännande och värdefulla språkliga upplevelser med hjälp av vardagsföremål och massor av fantasi. Boken är...

  15. Fabrication of DNA Microarrays on Polydopamine-Modified Gold Thin Films for SPR Imaging Measurements

    OpenAIRE

    Wood, Jennifer B.; Szyndler, Megan W.; Halpern, Aaron R.; Cho, Kyunghee; Corn, Robert M.

    2013-01-01

    Polydopamine (PDA) films were fabricated on thin film gold substrates in a single-step polymerization-deposition process from dopamine solutions and then employed in the construction of robust DNA microarrays for the ultra-sensitive detection of biomolecules with nanoparticle-enhanced surface plasmon resonance (SPR) imaging. PDA multilayers with thicknesses varying from 1 to 5 nm were characterized with a combination of scanning angle SPR and AFM experiments, and 1.3 ± 0.2 nm PDA multilayers ...

  16. Immune biosensors based on the SPR and TIRE: efficiency of their application for bacteria determination

    Science.gov (United States)

    Starodub, N. F.; Ogorodniichuk, J.; Lebedeva, T.; Shpylovyy, P.

    2013-11-01

    In this work we have designed high-specific biosensors for Salmonella typhimurium detection based on the surface plasmon resonance (SPR) and total internal reflection ellipsometry (TIRE). It has been demonstrated high selectivity and sensitivity of analysis. As a registering part for our experiments the Spreeta (USA) and "Plasmonotest" (Ukraine) with flowing cell have been applied among of SPR device. Previous researches confirmed an efficiency of SPR biosensors using for detecting of specific antigen-antibody interactions therefore this type of reactions with some previous preparations of surface binding layer was used as reactive part. It has been defined that in case with Spreeta sensitivity was on the level 103 - 107 cells/ml. Another biosensor based on the SPR has shown the sensitivity within 101 - 106 cells/ml. Maximal sensitivity was on the level of several cells in 10 ml (up to the fact that less than 5 cells) which has been obtained using the biosensor based on TIRE.

  17. Fabrication of DNA Microarrays on Polydopamine-Modified Gold Thin Films for SPR Imaging Measurements

    Science.gov (United States)

    Wood, Jennifer B.; Szyndler, Megan W.; Halpern, Aaron R.; Cho, Kyunghee

    2013-01-01

    Polydopamine (PDA) films were fabricated on thin film gold substrates in a single-step polymerization-deposition process from dopamine solutions and then employed in the construction of robust DNA microarrays for the ultra-sensitive detection of biomolecules with nanoparticle-enhanced surface plasmon resonance (SPR) imaging. PDA multilayers with thicknesses varying from 1 to 5 nm were characterized with a combination of scanning angle SPR and AFM experiments, and 1.3 ± 0.2 nm PDA multilayers were chosen as an optimal thickness for the SPR imaging measurements. DNA microarrays were then fabricated by the reaction of amine-functionalized single-stranded DNA (ssDNA) oligonucleotides with PDA-modified gold thin film microarray elements, and were subsequently employed in SPR imaging measurements of DNA hybridization adsorption and protein-DNA binding. Concurrent control experiments with noncomplementary ssDNA sequences demonstrated that the adhesive PDA multilayer was also able to provide good resistance to the nonspecific binding of biomolecules. Finally, a series of SPR imaging measurements of the hybridization adsorption of DNA-modified gold nanoparticles onto mixed sequence DNA microarrays were used to confirm that the use of PDA multilayer films is a simple, rapid and versatile method for fabricating DNA microarrays for ultrasensitive nanoparticle-enhanced SPR imaging biosensing. PMID:23902428

  18. Fabrication of DNA microarrays on polydopamine-modified gold thin films for SPR imaging measurements.

    Science.gov (United States)

    Wood, Jennifer B; Szyndler, Megan W; Halpern, Aaron R; Cho, Kyunghee; Corn, Robert M

    2013-08-27

    Polydopamine (PDA) films were fabricated on thin film gold substrates in a single-step polymerization-deposition process from dopamine solutions and then employed in the construction of robust DNA microarrays for the ultrasensitive detection of biomolecules with nanoparticle-enhanced surface plasmon resonance (SPR) imaging. PDA multilayers with thicknesses varying from 1 to 5 nm were characterized with a combination of scanning angle SPR and AFM experiments, and 1.3 ± 0.2 nm PDA multilayers were chosen as an optimal thickness for the SPR imaging measurements. DNA microarrays were then fabricated by the reaction of amine-functionalized single-stranded DNA (ssDNA) oligonucleotides with PDA-modified gold thin film microarray elements, and were subsequently employed in SPR imaging measurements of DNA hybridization adsorption and protein-DNA binding. Concurrent control experiments with non-complementary ssDNA sequences demonstrated that the adhesive PDA multilayer was also able to provide good resistance to the nonspecific binding of biomolecules. Finally, a series of SPR imaging measurements of the hybridization adsorption of DNA-modified gold nanoparticles onto mixed sequence DNA microarrays were used to confirm that the use of PDA multilayer films is a simple, rapid, and versatile method for fabricating DNA microarrays for ultrasensitive nanoparticle-enhanced SPR imaging biosensing.

  19. Mini-SOL Språka och Lek

    DEFF Research Database (Denmark)

    Bylander, Helle Iben

    Ju tidigare man börjar med en medveten språklig stimulering, desto bättre är det. I denna praktiska handbok finns pedagogiska tips och lekar för de allra yngsta barnen (0–3 år). Boken är sprängfull av roliga aktiviteter som alla stimulerar språkutvecklingen. Mini-SOL följer de språkliga områdena ...

  20. Biosensor analysis of the molecular interactions of pentosan polysulfate and of sulfated glycosaminoglycans with immobilized elastase, hyaluronidase and lysozyme using surface plasmon resonance (SPR) technology.

    Science.gov (United States)

    Shen, Bojiang; Shimmon, Susan; Smith, Margaret M; Ghosh, Peter

    2003-02-01

    Pentosan polysulfate (NaPPS) and chondroitin sulfates (ChSs) have recently been shown to exhibit both symptom and disease modifying activities in osteoarthritis (OA), but their respective mechanisms of action are still the subject of conjecture. Excessive catabolism of joint articular cartilage is considered to be responsible for the initiation and progression of OA but the abilities of these drugs to mitigate this process has received only limited attention. Human neutrophil elastase (HNE) is a proteinase, which can degrade the collagens and proteoglycans (PGs) of the cartilage directly or indirectly by activating latent matrix metalloproteinases. Hyaluronidase (HAase) is an endoglycosidase, which degrades glycosaminoglycans including hyaluronan, which provides the aggregating component of the PG aggrecan complex. In the present study the molecular interactions between the NaPPS, ChSs and some other sulfated polysaccharides with immobilized HNE, HAase or lysozyme (a cationic protein implicated in PG metabolism) were studied using a SPR biosensor device-BIAcore2000. The above three enzymes were covalently immobilized to a biosensor chip CM5 separately using amine coupling. The binding affinity of each sulfated polysaccharide and the kinetics of NaPPS over the concentration range of 0.3-5.0 microg/ml were determined. The inhibition of HNE by the sulfated polysaccharides as determined using the synthetic substrate succinyl-Ala-Ala-Val-nitroanilide (SAAVNA) in a functional assay was compared with their respective binding affinities for this proteinase using the BIAcore system. The results obtained with the two independent techniques showed good correlation and indicated that the degree and ring positions of oligosaccharide sulfation were major determinants of enzyme inhibitory activity. The observed difference in order of binding affinities of the drugs to the immobilized HNE, HAase and lysozyme suggests a conformational relationship, in addition to the charge

  1. Annual Report on SPR Operation in 2002

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    1 Information of operation SPR is operated for 2 727.983 h in 2002, the reactor’s rated power is 3.5 MW,and the total releasedenergy is 397.831 MW d Times of reactor’s operation and non-plan shut-down are 20 and 37,respectively. The pH and resistivity of primary loop water is 5.8 and 66.7×10~4Ω·cm, respectively;

  2. Metakognisjon om språk og språklæring i et flerspråklighetsperspektiv

    Directory of Open Access Journals (Sweden)

    Åsta Haukås

    2014-09-01

    Full Text Available I denne artikkelen drøfter jeg betydningen av elevers refleksjon om språk og språklæring. Artikkelens første del gir en kort introduksjon til forskningsfeltet metakognisjon. Deretter presenterer jeg to underkategorier av metakognisjon som er særlig relevante i språkundervisningen, metalingvistisk bevissthet og bevissthet om språklæringsstrategier. I artikkelens andre del introduserer jeg hovedprinsippene i flerspråklighetsdidaktikken, gir eksempler på hvordan elevene kan reflektere over språk og språklæring i språkfagene og argumenterer for at økt vekt på metakognisjon i og på tvers av språkfagene er en nøkkel til bedre språkkompetanse hos fremtidige elever. Dette krever imidlertid et sterkere samarbeid mellom språkfagene i skole, lærerutdanning og forskning.

  3. Integrated active mixing and biosensing using surface acoustic waves (SAW) and surface plasmon resonance (SPR) on a common substrate.

    Science.gov (United States)

    Renaudin, Alan; Chabot, Vincent; Grondin, Etienne; Aimez, Vincent; Charette, Paul G

    2010-01-07

    This article presents a device incorporating surface plasmon resonance (SPR) sensing and surface acoustic wave (SAW) actuation integrated onto a common LiNbO(3) piezoelectric substrate. The device uses Rayleigh-type SAW to provide active microfluidic mixing in the fluid above the SPR sensor. Validation experiments show that SAW-induced microfluidic mixing results in accelerated binding kinetics of an avidin-biotin assay. Results also show that, though SAW action causes a parasitic SPR response due to heat injection into the fluid, a relatively brief relaxation time following the SAW pulses allows the effect to dissipate, without affecting the overall assay response. Since both SPR sensors and SAW transducers can be fabricated simultaneously using low-cost microfabrication methods on a single substrate, the proposed design is well-suited to lab-on-chip applications.

  4. Thermodynamic characterization of pyrazole and azaindole derivatives binding to p38 mitogen-activated protein kinase using Biacore T100 technology and van't Hoff analysis.

    Science.gov (United States)

    Papalia, Giuseppe A; Giannetti, Anthony M; Arora, Nidhi; Myszka, David G

    2008-12-15

    Biacore T100 technology was used in conjunction with a van't Hoff analysis to characterize the thermodynamic binding parameters of 85 small-molecule inhibitors of adenosine triphosphate (ATP) binding to p38 mitogen-activated protein (MAP) kinase. The compounds were selected from a large panel of azaindole and pyrazole derivatives for which IC(50) data exist. We showed a strong relationship between the K(D) and IC(50) of a compound, but only a modest relationship between k(off) and IC(50) was detected and an apparent relationship between a compound's k(on) and its IC(50) could not be discerned. Similarly, a correlation between a compound's IC(50) and its thermodynamic parameters DeltaH degrees and DeltaS degrees could not be established. The lack of a predominant kinetic or thermodynamic signature associated with the inhibitory potential of these compounds demonstrates that there exists, even within a single well-defined system, a library of kinetic routes or, alternatively, a library of initial and final enthalpic and entropic states from which to effect inhibition. As a complement to these studies, selected double mutant thermodynamic cycles were performed to probe the energetic coupling, if any, between common sites of fluorination in both the azaindole and pyrazole classes and two different substituents. Although both cycles indicated negligible coupling free energies, both revealed significant coupling enthalpies, an observation made in other similarly dissected systems. The possible significance and caveats associated with these findings along with the advantages of using Biacore technology to derive thermodynamic parameters in drug discovery efforts are discussed.

  5. Annual Report of SPR in 2003

    Institute of Scientific and Technical Information of China (English)

    LIUCheng-hua

    2003-01-01

    1 Information of operation. SPR is operated for 1098.183 h in 2003, the reactor's rated power is 3.5 MW, and the total released energy is 159.442 MW·d. Times of reactor's operation and non-plan shut-down are 6 and 11, respectively.The pH and resistivity of primary loop water is 5.8 and 62.5×104 Ω·cm, respectively; maintaining pool water's pile and resisting is 6.5 and 15×104 Ω·cm, respectively. The exhausted gas waste is 1.308×107m3, 0.2063 TBq for 41Ar. Radioactive liquid waste is 11.25m3, e.g. 0.6431 GBq.

  6. Annual Report on SPR in 2001

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    1 Information of operationSPR is operated for 1 922.13 h in 2001, the reactor’s rated power is 3.5 MW, and the totalreleased energy is 280.311 MWd. Times of reactor’s operation and non-plan shut-down are 11 and 42, respectively. Primary loop water’s pH and resistivity is 5.9 and 71.5 × 104 Ω ·cm, respectively; maintaining pool water’s pile and resisting is 6.5 and 25 × 104 Ω · cm, respectively. The exhausted gas waste is 2.287 2× 107m3, e.g. 0.370 88 TBq for 2.1Ar. Radioactive liquid waste is 21.9 m3, e.g. 0.123 86 GBq.

  7. An SPR based sensor for allergens detection.

    Science.gov (United States)

    Ashley, J; Piekarska, M; Segers, C; Trinh, L; Rodgers, T; Willey, R; Tothill, I E

    2017-02-15

    A simple, sensitive and label-free optical sensor method was developed for allergens analysis using α-casein as the biomarker for cow's milk detection, to be used directly in final rinse samples of cleaning in place systems (CIP) of food manufacturers. A Surface Plasmon Resonance (SPR) sensor chip consisting of four sensing arrays enabling the measurement of samples and control binding events simultaneously on the sensor surface was employed in this work. SPR offers several advantages in terms of label free detection, real time measurements and superior sensitivity when compared to ELISA based techniques. The gold sensor chip was used to immobilise α-casein-polyclonal antibody using EDC/NHS coupling procedure. The performance of the assay and the sensor was first optimised and characterised in pure buffer conditions giving a detection limit of 58ngmL(-1) as a direct binding assay. The assay sensitivity can be further improved by using sandwich assay format and amplified with nanoparticles. However, at this stage this is not required as the detection limit achieved exceeded the required allergens detection levels of 2µgmL(-1) for α-S1-casein. The sensor demonstrated good selectivity towards the α-casein as the target analyte and adequate recoveries from CIP final rinse wash samples. The sensor would be useful tool for monitoring allergen levels after cleaning procedures, providing additional data that may better inform upon wider food allergen risk management decision(s) that are made by food manufacturer. In particular, this sensor could potentially help validate or optimise cleaning practices for a given food manufacturing process.

  8. Lingvistik, dialogism, hermeneutik: Om språkvetenskapens kunskapsobjekt

    Directory of Open Access Journals (Sweden)

    Andreas Widoff

    2012-04-01

    Full Text Available En stor del av språkvetenskapen vilar sedan länge på en snäv idé om sitt kunskapsobjekt. Inställningen är nomotetisk och logocentrisk. Med språk avses ett regelbundet system. Fastän denna inställning mött mycket motstånd, har den väl i praktiken aldrig skakats i grunden. Denna undersökning försöker därför ännu en gång att presentera ett alternativt språkbegrepp. Teorin är dialogisk och hermeneutisk: den språkliga betydelsens plats i världen och tolkningen som grunden för all betydelse är vägledande principer. Genom dessa principer introduceras två begreppsliga ramverk. Det första lägger grunden för en kontinuerlig språkmodell som försöker att upplösa dikotomin mellan system och bruk. Till denna modell fogas sedan en tolkningstypologi. Denna har ett dubbelt syfte. Å ena sidan möjliggör den en systematisk jämförelse av språkvetenskapens discipliner. Å andra sidan är den en beskrivning av språkets betydelsestruktur.

  9. Subtilase SprP exerts pleiotropic effects in Pseudomonas aeruginosa.

    Science.gov (United States)

    Pelzer, Alexander; Polen, Tino; Funken, Horst; Rosenau, Frank; Wilhelm, Susanne; Bott, Michael; Jaeger, Karl-Erich

    2014-02-01

    The open reading frame PA1242 in the genome of Pseudomonas aeruginosa PAO1 encodes a putative protease belonging to the peptidase S8 family of subtilases. The respective enzyme termed SprP consists of an N-terminal signal peptide and a so-called S8 domain linked by a domain of unknown function (DUF). Presumably, this DUF domain defines a discrete class of Pseudomonas proteins as homologous domains can be identified almost exclusively in proteins of the genus Pseudomonas. The sprP gene was expressed in Escherichia coli and proteolytic activity was demonstrated. A P. aeruginosa ∆sprP mutant was constructed and its gene expression pattern compared to the wild-type strain by genome microarray analysis revealing altered expression levels of 218 genes. Apparently, SprP is involved in regulation of a variety of different cellular processes in P. aeruginosa including pyoverdine synthesis, denitrification, the formation of cell aggregates, and of biofilms.

  10. Near orthogonal launch of SPR modes in Au films

    CERN Document Server

    Canning, John; Tzoumis, Nikki; Tan, Yanzhen; Patyk, Rodolfo; Gibson, Brant C

    2014-01-01

    We report the excitation of a surface plasmon resonance (SPR) close to the orthogonal axis of a gold (Au) film on borosilicate glass. Direct spectroscopic measurement of SPR shifts using different liquids are made at ~5{\\deg} incidence within a reflection spectrophotometer. Scattering of light that is able to penetrate across the film at the interfaces is the proposed mechanism by which coupling, and plasmon localization, is established.

  11. Polystyrene Based SPR Biosensor Chip for Use in Immunoassay

    Institute of Scientific and Technical Information of China (English)

    1999-01-01

    Biosensors are widely used in immunoassay.The biosensor chip carries a receptor which is used in immunoassay and the chip properties have an important influence on the detecting sensitivity of the biosensor.This paper describes a polystyrene-based biosensor chip developed and used as part of a surface plasmon resonance (SPR) biosensor.The SPR biosensor has a much higher detecting sensitivity than enzyme-linked immunoserbent assay (ELISA).

  12. Influence of calcium blockers on the SPR of erythrocytes

    Science.gov (United States)

    Shynkarenko, Olena V.; Tril, Orest; Wojnarowska, Renata; Prohorenko, Sergiy; Shergii, E. M.

    2016-12-01

    One of the promising areas of research is the impact of calcium channel blockers (CB) of biological fluids. This paper shows that the CB impact on a biological fluid can be efficiently combine with the surface plasmon resonance (SPR). It is shown that the addition of CB at the SPR measurements affect the stability of membranes and acts differently on the kinetics of erythrocytes ligament in the different groups of people.

  13. Nano Sensing and Energy Conversion Using Surface Plasmon Resonance (SPR

    Directory of Open Access Journals (Sweden)

    Iltai (Isaac Kim

    2015-07-01

    Full Text Available Nanophotonic technique has been attracting much attention in applications of nano-bio-chemical sensing and energy conversion of solar energy harvesting and enhanced energy transfer. One approach for nano-bio-chemical sensing is surface plasmon resonance (SPR imaging, which can detect the material properties, such as density, ion concentration, temperature, and effective refractive index in high sensitivity, label-free, and real-time under ambient conditions. Recent study shows that SPR can successfully detect the concentration variation of nanofluids during evaporation-induced self-assembly process. Spoof surface plasmon resonance based on multilayer metallo-dielectric hyperbolic metamaterials demonstrate SPR dispersion control, which can be combined with SPR imaging, to characterize high refractive index materials because of its exotic optical properties. Furthermore, nano-biophotonics could enable innovative energy conversion such as the increase of absorption and emission efficiency and the perfect absorption. Localized SPR using metal nanoparticles show highly enhanced absorption in solar energy harvesting. Three-dimensional hyperbolic metamaterial cavity nanostructure shows enhanced spontaneous emission. Recently ultrathin film perfect absorber is demonstrated with the film thickness is as low as ~1/50th of the operating wavelength using epsilon-near-zero (ENZ phenomena at the wavelength close to SPR. It is expected to provide a breakthrough in sensing and energy conversion applications using the exotic optical properties based on the nanophotonic technique.

  14. Læring om normering. Erfaringer fra et språkhistorie-didaktisk eksperiment i lærerutdanninga

    Directory of Open Access Journals (Sweden)

    Stian Hårstad

    2016-09-01

    Full Text Available I denne artikkelen beskrives utprøvinga av et didaktisk opplegg i grunnskolelærerutdanningas norskfag som har språkhistorie som overordnet tema. Målet med metodikken som legges fram, er å gi innsikt i språknormering som sosiokulturell prosess for på denne måten å utvikle en forståelse av hvordan språkhistorie har blitt og fortsatt blir skapt. Artikkelen drøfter hvordan en slik tilnærming kan gi deltakerne økt historisk bevissthet om språklige fenomener. Samtidig presenteres kunnskap om hvilke normeringsprinsipper de involverte lærerstudentene intuitivt ser som de mest “naturlige” og “hensiktsmessige” i en språkplanleggingskontekst. Et hovedfunn er at det store flertallet løfter fram et “demokrati- eller jamlikhetsprinsipp” og et “ortofoniprinsipp” som de mest tungtveiende. Artikkelen kommer dessuten med forslag til hvordan det språkhistorie-didaktiske feltet kan utforskes videre. Nøkkelord: språkhistorie-didaktikk, språkdidaktikk, språkhistorie, normeringshistorie, historiebevissthetAbstract This article presents a didactic experiment in the subject Norwegian as L1 in the primary and lower secondary teacher education programmes [Grunnskolelærerutdanninga]. The objective of the proposed method is to give insight into language normalization as a sociocultural process and thereby develop an understanding of how language history has been and still is being shaped. The paper discusses how this approach may provide the participants with an increased language historical consciousness. Moreover, the article presents findings about which normalization principles the student teachers intuitively regard as the most “natural” and “appropriate” in a language-planning context. A main finding is that the great majority designates a “democracy and equality principle” along with an “orthographic principle” as the two most substantial ones. Additionally, the article suggests how the field of language

  15. Immobilization of homogeneous monomeric, oligomeric and fibrillar Aβ species for reliable SPR measurements.

    Science.gov (United States)

    Frenzel, Daniel; Glück, Julian M; Brener, Oleksandr; Oesterhelt, Filipp; Nagel-Steger, Luitgard; Willbold, Dieter

    2014-01-01

    There is strong evidence that the amyloid-beta peptide (Aβ) plays a central role in the pathogenesis of Alzheimer's disease (AD). In this context, a detailed quantitative description of the interactions with different Aβ species is essential for characterization of physiological and artificial ligands. However, the high aggregation propensity of Aβ in concert with its susceptibility to structural changes due to even slight changes in solution conditions has impeded surface plasmon resonance (SPR) studies with homogeneous Aβ conformer species. Here, we have adapted the experimental procedures to state-of-the-art techniques and established novel approaches to reliably overcome the aforementioned challenges. We show that the application of density gradient centrifugation (DGC) for sample purification and the use of a single chain variable fragment (scFv) of a monoclonal antibody directed against the amino-terminus of Aβ allows reliable SPR measurements and quality control of the immobilized Aβ aggregate species at any step throughout the experiment.

  16. Immobilization of homogeneous monomeric, oligomeric and fibrillar Aβ species for reliable SPR measurements.

    Directory of Open Access Journals (Sweden)

    Daniel Frenzel

    Full Text Available There is strong evidence that the amyloid-beta peptide (Aβ plays a central role in the pathogenesis of Alzheimer's disease (AD. In this context, a detailed quantitative description of the interactions with different Aβ species is essential for characterization of physiological and artificial ligands. However, the high aggregation propensity of Aβ in concert with its susceptibility to structural changes due to even slight changes in solution conditions has impeded surface plasmon resonance (SPR studies with homogeneous Aβ conformer species. Here, we have adapted the experimental procedures to state-of-the-art techniques and established novel approaches to reliably overcome the aforementioned challenges. We show that the application of density gradient centrifugation (DGC for sample purification and the use of a single chain variable fragment (scFv of a monoclonal antibody directed against the amino-terminus of Aβ allows reliable SPR measurements and quality control of the immobilized Aβ aggregate species at any step throughout the experiment.

  17. Floating Chip Mounting System Driven by Repulsive Force of Permanent Magnets for Multiple On-Site SPR Immunoassay Measurements

    Directory of Open Access Journals (Sweden)

    Emi Tamechika

    2012-10-01

    Full Text Available We have developed a measurement chip installation/removal mechanism for a surface plasmon resonance (SPR immunoassay analysis instrument designed for frequent testing, which requires a rapid and easy technique for changing chips. The key components of the mechanism are refractive index matching gel coated on the rear of the SPR chip and a float that presses the chip down. The refractive index matching gel made it possible to optically couple the chip and the prism of the SPR instrument easily via elastic deformation with no air bubbles. The float has an autonomous attitude control function that keeps the chip parallel in relation to the SPR instrument by employing the repulsive force of permanent magnets between the float and a float guide located in the SPR instrument. This function is realized by balancing the upward elastic force of the gel and the downward force of the float, which experiences a leveling force from the float guide. This system makes it possible to start an SPR measurement immediately after chip installation and to remove the chip immediately after the measurement with a simple and easy method that does not require any fine adjustment. Our sensor chip, which we installed using this mounting system, successfully performed an immunoassay measurement on a model antigen (spiked human-IgG in a model real sample (non-homogenized milk that included many kinds of interfering foreign substances without any sample pre-treatment. The ease of the chip installation/removal operation and simple measurement procedure are suitable for frequent on-site agricultural, environmental and medical testing.

  18. Floating chip mounting system driven by repulsive force of permanent magnets for multiple on-site SPR immunoassay measurements.

    Science.gov (United States)

    Horiuchi, Tsutomu; Tobita, Tatsuya; Miura, Toru; Iwasaki, Yuzuru; Seyama, Michiko; Inoue, Suzuyo; Takahashi, Jun-ichi; Haga, Tsuneyuki; Tamechika, Emi

    2012-10-17

    We have developed a measurement chip installation/removal mechanism for a surface plasmon resonance (SPR) immunoassay analysis instrument designed for frequent testing, which requires a rapid and easy technique for changing chips. The key components of the mechanism are refractive index matching gel coated on the rear of the SPR chip and a float that presses the chip down. The refractive index matching gel made it possible to optically couple the chip and the prism of the SPR instrument easily via elastic deformation with no air bubbles. The float has an autonomous attitude control function that keeps the chip parallel in relation to the SPR instrument by employing the repulsive force of permanent magnets between the float and a float guide located in the SPR instrument. This function is realized by balancing the upward elastic force of the gel and the downward force of the float, which experiences a leveling force from the float guide. This system makes it possible to start an SPR measurement immediately after chip installation and to remove the chip immediately after the measurement with a simple and easy method that does not require any fine adjustment. Our sensor chip, which we installed using this mounting system, successfully performed an immunoassay measurement on a model antigen (spiked human-IgG) in a model real sample (non-homogenized milk) that included many kinds of interfering foreign substances without any sample pre-treatment. The ease of the chip installation/removal operation and simple measurement procedure are suitable for frequent on-site agricultural, environmental and medical testing.

  19. Analysis of benzoyl-peroxide and formaldehyde as dental allergens by FT-SPR method

    Science.gov (United States)

    Bako, J.; Kelemen, M.; Hegedus, Cs

    2013-12-01

    In parallel with the appearance of new dental materials the number of induced allergic diseases increases. Based on this fact more sensitive detection of allergens is major importance. The Fourier-Transform Surface Plasmon Resonance (FT-SPR) is a sensitive, broadly applicable real-time method for analysing thin layers of materials on gold surfaces. FT-SPR measurement is performed at a fixed angel of incident light, and reflectivity is measured over a range of wavelength in the near infrared. In our study the formaldehyde and benzoyl-peroxide were examined as members of the most common dental allergens by FT-SPR spectroscopy. The aim of this work was the investigation of the suitability of this method for the direct detection of these materials. Different concentrations of formaldehyde and benzoyl-peroxide solutions were measured from this purpose. The individual spectra were measured for all of the solutions, and calibration curves were calculated for the materials for the possibility of the determination of an unknown concentration. In addition, series measurements were performed whereby the association and dissociation properties of formaldehyde or benzoyl-peroxide were described. The results of the experiments proved that the method capable to measure directly these materials and can provide appropriate calibration curves for determination of unknown concentrations.

  20. Estimation of optical constants of a bio-thin layer (onion epidermis), using SPR spectroscopy

    Science.gov (United States)

    Rehman, Saif-ur-; Mumtaz, Huma; Hayashi, Shinji; Shaukat, S. F.; Sekkat, Zouheir

    2014-12-01

    We estimate the optical constants of a biological thin layer (Allium cepa) by surface plasmon resonance (SPR) spectroscopy. For this study, the fresh inner thin epidermis of an onion bulb was used and stacked directly on gold (Au) and silver (Ag) film surfaces in order to identify the shift in SPR mode of each metal film at an operating wavelength of 632.8 nm. The thickness and dielectric constants of the biological thin layer were determined by matching the experimental SPR curves to theoretical ones. The thickness and roughness of bare Au and Ag thin films were also measured by atomic force microscopy (AFM); the results of which are in good agreement with those obtained through experiment. Due to the high surface roughness of the natural onion epidermis layer, AFM could not measure the exact thickness of an onion epidermis. It is estimated that the value of the real part of the dielectric constant of an onion epidermis is between the dielectric constants of water and air.

  1. SPR and SPR Imaging: Recent Trends in Developing Nanodevices for Detection and Real-Time Monitoring of Biomolecular Events

    Directory of Open Access Journals (Sweden)

    Mihaela Puiu

    2016-06-01

    Full Text Available In this paper we review the underlying principles of the surface plasmon resonance (SPR technique, particularly emphasizing its advantages along with its limitations regarding the ability to discriminate between the specific binding response and the interfering effects from biological samples. While SPR sensors were developed almost three decades, SPR detection is not yet able to reduce the time-consuming steps of the analysis, and is hardly amenable for miniaturized, portable platforms required in point-of-care (POC testing. Recent advances in near-field optics have emerged, resulting in the development of SPR imaging (SPRi as a powerful optical, label-free monitoring tool for multiplexed detection and monitoring of biomolecular events. The microarrays design of the SPRi chips incorporating various metallic nanostructures make these optofluidic devices more suitable for diagnosis and near-patient testing than the traditional SPR sensors. The latest developments indicate SPRi detection as being the most promising surface plasmon-based technique fulfilling the demands for implementation in lab-on-a-chip (LOC technologies.

  2. Strategies for the detection of food pathogens and contaminants

    DEFF Research Database (Denmark)

    Hearty, Stephen; Leonard, Paul; Sheehan, Alfredo Darmanin

    molecules and once suitably high affinity antibodies have been isolated, it is relatively straightforward to design and optimise concentration-based assays using SPR. Recently we have investigated the potential of applying Biacore technology to routine food analysis. Our experiences have shown...... that molecular contaminants such as microbial toxins and drug/pesticide residues translate well onto Biacore-based assay formats. However, larger and more complex entities such as spores and whole bacterial cells represent an altogether more difficult challenge. Here, we present an overview of our experiences...... to date with using Biacore for analysis of food contaminants and in particular the challenges associated with large analyte detection...

  3. Experimental Studies on Biosensing by SPR

    Science.gov (United States)

    Sun, Xiaochun; Shiokawa, Showko; Matsui, Yosikazu

    1989-09-01

    Biosensing using surface plasmon resonance is experimentally studied. The results obtained by detecting the bioresponse of the α-Feto protein system and cyclic experiments show that this technique has potential application in the field of biosensing. The effects of an organic layer on detecting sensitivity and restoration of sensors are also discussed.

  4. Hybrid metallic ion-exchanged waveguides for SPR biological sensing

    Science.gov (United States)

    de Bonnault, S.; Bucci, D.; Zermatten, P.. J.; Charette, P. G.; Broquin, J. E.

    2015-02-01

    Glass substrates have been used for decades to create biosensors due to their biocompatibility, low thermal conductivity, and limited fluorescence. Among the different types of sensors, those based on surface plasmon resonance (SPR) allow exploitation of the sensing lightwave at the vicinity of the sensor surface where small entities such as DNA or proteins are located. In this paper, ion-exchanged waveguides and SPR are combined to create a multianalyte optical sensor integrated onto glass. First the principle of operation is introduced, then the theoretical analysis and design of the sensing element. Simulations have been carried out using the Aperiodic Fourier Modal Method (AFMM) and a custom software that handles ion-exchange index-profiles. Fabrication and characterization processes are also presented. Finally the first experimental spectra are displayed and discussed. The sensor presents a bulk sensibility of 5000nm/RIU.

  5. A new silver nanorod SPR probe for detection of trace benzoyl peroxide

    Science.gov (United States)

    Jiang, Zhiliang; Wen, Guiqing; Luo, Yanghe; Zhang, Xinghui; Liu, Qingye; Liang, Aihui

    2014-06-01

    The stable silver nanorod (AgNR) sol in red was prepared by the two-step procedure of NaBH4-H2O2 and citrate heating reduction. The AgNR had a transverse and a longitudinal surface plasmon resonance (SPR) absorption peak at 338 nm and 480 nm. Meanwhile, two transverse and longitudinal SPR Rayleigh scattering (SPR-RS) peaks at 340 nm and 500 nm were observed firstly using common fluorescence spectrometer. The SPR absorption, RS, surface enhanced Raman scattering (SERS) and electron microscope technology were used to study the formation mechanism of red silver nanorods and the SERS enhancement mechanism of nano-aggregation. The AgNR-BPO SPR absorption and AgNR-NaCl-BPO SPR-RS analytical systems were studied to develop two new simple, rapid, and low-cost SPR methods for the detection of trace BPO.

  6. Combined SPR and SERS: Otto and Kretschmann configurations

    Science.gov (United States)

    Barchiesi, Dominique; Grosges, Thomas; Colas, Florent; Lamy de la Chapelle, Marc

    2015-11-01

    Combined surface enhanced Raman spectroscopy (SERS) and surface plasmon resonance (SPR) setup was proposed recently. The experimental setup requires a gold layer deposited on a glass substrate. However, due to the poor ability of sticking gold material on glass, a nanometric adhesion layer is used. In this paper we compare numerically both the SERS gain, and the SPR signal, for metallic and dielectric adhesion layers, for two substrates. We show that even if the dielectric materials can be considered as equivalent for the SPR signal, this is not the case for the SERS gain. In particular the dielectric adhesion layer reduces the sensitivity of the SERS gain to this parameter and therefore their use could be more suitable for the fabrication of the sensor. Moreover the higher the refractive index of substrate with regards to the adhesion layer is, the higher efficiency of setup is obtained, and therefore the Otto configuration seems to be more efficient than the Kretschmann one. Optimization of the thicknesses of the adhesion layer and of the gold layer can lead to a SERS gain greater than 103 without nanostructuring.

  7. 基于高斯拟合的光纤型 SPR 信号的峰值检测算法%Peak Detection Algorithm of Optical Fiber SPR Signal Based on the Gaussian Fitting

    Institute of Scientific and Technical Information of China (English)

    周鹏; 张文斌; 王军星; 孙翠迎; 刘瑾; 苏荣欣; 王学民

    2016-01-01

    The spectrum of optical fiber surface plasmon resonance (SPR) shows the characteristics of wider full width at half‐maximum (FWHM ) and smaller peak value .The traditional peak detection algorithm cannot calculate the resonance wavelength of this kind of spectrum accurately .Therefore ,efficient methods to calculate the resonance wavelength are required .This paper presents a method to process the SPR signal based on the Gaussian fitting .Combined with the trust region algorithm to deter‐mine the Gaussian fitting function ,and then the resonance wavelength be calculated by line search method .Data processing of standard spectrum of glycerin with different concentration proves that this algorithm can adapt to optical fiber SPR spectrum characteristics ,and calculate resonance wavelength accurately .The experiment measures the SPR signals of sucrose under differ‐ent concentrations through the self‐building SPR measure system .By using the proposed method ,the weighted centroid method and the tracking centroid method to calculate the resonance wavelength ,the result shows that the Gaussian fitting method can ef ‐fectively improve resolution and have a short operation time which is conducive to engineering application .%光纤型表面等离子共振光谱具有半峰宽较宽,共振峰不尖锐等特征,传统的针对棱镜型 SPR 光谱的峰值检测算法无法准确定位此类光谱的共振峰值。为了准确计算光纤型 SPR 光谱的共振波长,提出了一种基于信赖域算法的高斯拟合方法,在拟合的光谱曲线上利用一维搜索的方式确定峰值位置。通过对标准甘油光谱数据的处理,证明高斯拟合法能够适应光纤型 SPR 光谱的特征,波长计算准确。通过搭建的 SPR系统,实验测量了不同浓度下蔗糖溶液的 SPR 光谱数据,并分别采用加权质心法,追踪质心法和该研究提出的高斯拟合法进行共振波长的计算。结果表明高斯拟合法

  8. Exploiting Surface Plasmon Resonance (SPR Technology for the Identification of Fibroblast Growth Factor-2 (FGF2 Antagonists Endowed with Antiangiogenic Activity

    Directory of Open Access Journals (Sweden)

    Marco Presta

    2009-08-01

    Full Text Available Angiogenesis, the process of new blood vessel formation, is implicated in various physiological/pathological conditions, including embryonic development, inflammation and tumor growth. Fibroblast growth factor-2 (FGF2 is a heparin-binding angiogenic growth factor involved in various physiopathological processes, including tumor neovascularization. Accordingly, FGF2 is considered a target for antiangiogenic therapies. Thus, numerous natural/synthetic compounds have been tested for their capacity to bind and sequester FGF2 in the extracellular environment preventing its interaction with cellular receptors. We have exploited surface plasmon resonance (SPR technique in search for antiangiogenic FGF2 binders/antagonists. In this review we will summarize our experience in SPR-based angiogenesis research, with the aim to validate SPR as a first line screening for the identification of antiangiogenic compounds.

  9. Applications of optical fiber SPR sensor for measuring of temperature and concentration of liquids

    Science.gov (United States)

    Zeng, Jie; Liang, Dakai; Cao, Zhenxin

    2005-05-01

    A novel optical fiber sensor based on surface plasmon resonance phenomenon that can be used for liquid temperature testing around the sensing probe is presented. According to the attribute of liquid refractive index decreasing with the rise of liquid temperature, a series of SPR spectrum curves, corresponding to temperature range from 10 °C to 90 °C, can be obtained from spectrum signal processing. The range of temperature testing can be adjusted by choosing liquids with different boil and refractive index as environmental medium. This paper analyzes theoretically the influence of a variety of liquid temperature on the metal film coated onto the sensing probe surface and the refractive index of fiber core. A real-time double-parameter testing involving resonance wavelength and the lowest light intensity reflectivity is also proposed. Moreover, the proportion concentration of mixed solutions with different refractive indices ranging from 1.3675 to 1.4281 refractive index units, including ethanol and glycol, is investigated utilizing relative spectrum measurement technology. The preliminary experiments confirm the feasibility of the optical fiber SPR sensor based on wavelength-interrogation for temperature and refractive index measurement.

  10. SPR imaging combined with cyclic voltammetry for the detection of neural activity

    Directory of Open Access Journals (Sweden)

    Hui Li

    2014-03-01

    Full Text Available Surface plasmon resonance (SPR detects changes in refractive index at a metal-dielectric interface. In this study, SPR imaging (SPRi combined with cyclic voltammetry (CV was applied to detect neural activity in isolated bullfrog sciatic nerves. The neural activities induced by chemical and electrical stimulation led to an SPR response, and the activities were recorded in real time. The activities of different parts of the sciatic nerve were recorded and compared. The results demonstrated that SPR imaging combined with CV is a powerful tool for the investigation of neural activity.

  11. Three dimensional simulation for bayou choctaw strategic petroleum reserve (SPR).

    Energy Technology Data Exchange (ETDEWEB)

    Ehgartner, Brian L. (Sandia National Laboratories, Albuquerque, NM); Park, Byoung Yoon; Lee, Moo Yul

    2006-12-01

    Three dimensional finite element analyses were performed to evaluate the structural integrity of the caverns located at the Bayou Choctaw (BC) site which is considered a candidate for expansion. Fifteen active and nine abandoned caverns exist at BC, with a total cavern volume of some 164 MMB. A 3D model allowing control of each cavern individually was constructed because the location and depth of caverns and the date of excavation are irregular. The total cavern volume has practical interest, as this void space affects total creep closure in the BC salt mass. Operations including both cavern workover, where wellhead pressures are temporarily reduced to atmospheric, and cavern enlargement due to leaching during oil drawdowns that use water to displace the oil from the caverns, were modeled to account for as many as the five future oil drawdowns in the six SPR caverns. The impacts on cavern stability, underground creep closure, surface subsidence, infrastructure, and well integrity were quantified.

  12. Imaging Array SPR Biosensor Immunoassays for Sulfamethoxazole and Sulfamethazine

    Institute of Scientific and Technical Information of China (English)

    Hui LI; Da Fu CUI; Jin Qing LIANG; HaoYuan CAI; Yu Jie WANG

    2006-01-01

    A homemade array surface plasmon resonance (SPR)-based imaging biosensor was used to develop sensitive and fast immunoassays to determine sulfamethoxazole (SMOZ) and sulfamethazine (SMT) in buffer. Two conjugations of sulfonamide-bovine serum albumin (BSA)were separately immobilized on two different rows of the array chip with one row as reference.The immobilization was carried out in the instrument to monitor the quantity of the conjugations immobilized. The antibody mixed with the sulfonamide in the buffer was injected over the surface of the chip to get a relative response which was inversely proportional to the concentration of the sulfonamide in the PBS buffer. Two calibration curves were constructed and the limit of detection for sufamethoxazole in buffer was 3.5 ng/mL and for sulfamethazine 0.6 ng/mL. The stability and specificity of the antibody were also studied. The monoclonal antibody did not bind with BSA.

  13. A C-terminal truncated mutation of spr-3 gene extends lifespan in Caenorhabditis elegans

    Institute of Scientific and Technical Information of China (English)

    Ping Yang; Ruilin Sun; Minghui Yao; Weidong Chen; Zhugang Wang; Jian Fei

    2013-01-01

    The lifespan of Caenorhabditis elegans is determined by various genetic and environmental factors.In this paper,spr-3,a C.elegans homologous gene of the mammalian neural restrictive silencing factor (NRSF/REST),is reported to be an important gene regulating lifespan of C.elegans.A deletion mutation ofspr-3,spr-3(ok2525),or RNAi inhibition of spr-3 expression led to the short lifespan phenotype in C.elegans.However,a nonsense mutation of spr-3,spr3(by108),increased the lifespan by 26% when compared with that of wild-type nematode.The spr-3(by108) also showed increased resistance to environmental stress.The spr-3(by108) mutated gene encodes a C-terminal truncated protein with a structure comparable with the REST4,a splice variant of the NRSF/REST in mammalian.The long lifespan phenotype of spr-3(by108) mutant is confirmed as a gain of function and dependent on normal functions of daf16 and glp-1.The lifespan of the spr-3(by108) can be synergistically enhanced by inducing a mutation in daf-2.Quantitative polymerase chain reaction results showed that the expression of daf-16 as well as its target gene sod-3,mtl1,and sip-1 was up-regulated in the spr-3(by108) mutant.These results would be helpful to further understand the complex function of NRSF/REST gene in mammalian,especially in the aging process and longevity determination.

  14. Electrochemical Surface Plasmon Resonance (EC-SPR) and Waveguide Enhanced Glucose Biosensing with N-Alkylaminated Polypyrrole/Glucose Oxidase Multilayers

    Science.gov (United States)

    Baba, Akira; Taranekar, Prasad; Ponnapati, Ramakrishna R.; Knoll, Wolfgang; Advincula, Rigoberto C.

    2010-01-01

    In this work, we report an electrochemical surface plasmon resonance/waveguide (EC-SPR/waveguide) glucose biosensor, which could detect enzymatic reactions in a conducting polymer/glucose oxidase (GOx) multilayer thin film. In order to achieve a controlled enzyme electrode and waveguide mode, GOx (negatively charged) was immobilized with a water-soluble conducting N-alkylaminated polypyrrole (positively charged) using the layer-by-layer (LbL) electrostatic self-assembly technique. The electrochemical and optical signals were simultaneously obtained from the composite LbL enzyme electrode upon addition of glucose as mediated by the electroactivity and electrochromic property of the polypyrrole layers. The signal enhancement in the EC-SPR detection is obtained by monitoring the doping-dedoping events on the polypyrrole. The real time optical signal could be distinguished between the change in the dielectric constant of the enzyme layer and other non-enzymatic reaction events such as adsorption of glucose and change of refractive index of solution. This was possible by a correlation of both the SPR mode, m=0, and m=1 mode of the waveguide in an SPR/waveguide spectroscopy experiment. PMID:20666478

  15. Analysis of cavern stability at the West Hackberry SPR site.

    Energy Technology Data Exchange (ETDEWEB)

    Ehgartner, Brian L.; Sobolik, Steven Ronald

    2009-05-01

    This report presents computational analyses that simulate the structural response of caverns at the Strategic Petroleum Reserve (SPR) West Hackberry site. The cavern field comprises 22 caverns. Five caverns (6, 7, 8, 9, 11) were acquired from industry and have unusual shapes and a history dating back to 1946. The other 17 caverns (101-117) were leached according to SPR standards in the mid-1980s and have tall cylindrical shapes. The history of the caverns and their shapes are simulated in a three-dimensional geomechanics model of the site that predicts deformations, strains, and stresses. Future leaching scenarios corresponding to oil drawdowns using fresh water are also simulated by increasing the volume of the caverns. Cavern pressures are varied in the model to capture operational practices in the field. The results of the finite element model are interpreted to provide information on the current and future status of subsidence, well integrity, and cavern stability. The most significant results in this report are relevant to Cavern 6. The cavern is shaped like a bowl with a large ceiling span and is in close proximity to Cavern 9. The analyses predict tensile stresses at the edge of the ceiling during repressuization of Cavern 6 following workover conditions. During a workover the cavern is at low pressure to service a well. The wellhead pressures are atmospheric. When the workover is complete, the cavern is repressurized. The resulting elastic stresses are sufficient to cause tension around the edge of the large ceiling span. With time, these stresses relax to a compressive state because of salt creep. However, the potential for salt fracture and propagation exists, particularly towards Cavern 9. With only 200 ft of salt between the caverns, the operational consequences must be examined if the two caverns become connected. A critical time may be during a workover of Cavern 9 in part because of the operational vulnerabilities, but also because dilatant damage is

  16. QCM and EC-SPR Studies of Cytochrome c Self-assembled on Au Electrode and Enhancement of SPR Signal by Au Nanoparticles

    Institute of Scientific and Technical Information of China (English)

    WANG Yan-yan; JIANG Yan-xia; ZHOU You-chen; LI Yan-yan; MA Zhi-fang; SUN Shi-gang

    2012-01-01

    Quartz crystal microbalance(QCM) and cyclic voltammetry(CV) were used to characterize the monolayer of cytochrome c(Cyt c),which was adsorbed on gold film modified with alkanethiol mixed monolayer.A direct comparison of protein surface coverages calculated from QCM and cyclic voltammetric measurements illustrates that the ratio of the electroactive Cyt c to the total surface-confined Cyt c is 34%,which suggests that the orientation is a main factor affecting the electroactivity of Cyt c.Moreover,surface plasmon resonance(SPR) measurement combined with CV "in situ" was used to investigate the conformational change of Cyt c in the redox process.Besides,Au nanoparticles(Au NPs) were adsorbed on the surface of Cyt c.The result indicates that Au NPs promote electron transfer between Cyt c and the gold electrode,and SPR result suggests Au NPs enhance SPR signal.

  17. Large dynamic range SPR measurements in the visible using a ZnSe prism

    CERN Document Server

    Canning, John; Cook, Kevin

    2015-01-01

    Large dynamic index measurement range (n = 1 to n = 1.7) using surface plasmon resonance (SPR) shifts is demonstrated with a ZnSe prism at 632.8 nm, limited by the available high index liquid hosts. In contrast to borosilicate based SPR measurements where angular limitations restrict solvent use to water and requires considerable care dealing with Fresnel reflections, the ZnSe approach allows SPR spectroscopies to be applied to a varied range of solvents An uncertainty in angular resolution between 1.5 and 6 deg, depending on the solvent and SPR angle, was estimated. The refractive index change for a given glucose concentration in water was measured to be n = (0.114 to 0.007) per precentage C6H12O6 conc. Given the transmission properties of ZnSe the processes can be readily extended into the mid infrared.

  18. Arkitekturen som brukskonst:Husens språk revisited

    OpenAIRE

    Nyman, K

    2006-01-01

    Sammanfattning I min avhandling Husens språk (1989) framförde jag en hypotes om arkitekturens språkliga karaktär: eftersom den är språk, kan arkitekturen bli en central ingrediens i vars och ens mänsklighet på samma sätt som modersmålet. Jag konstaterade också, att den nutida arkitekturen till största delen för de flesta människor är som ett främmande språk. Genom åren har jag gått vidare med avhandlingens teman, bl.a. i mina böcker Talojen kieli (1998) och Sinisilmäisyyden aika ...

  19. Surface functionalization of SPR chip for specific molecular interaction analysis under flow condition

    OpenAIRE

    Tao Ma; Xiaoxia Chen; Qing Peng; Pengfei Zhang; Yonghong He

    2017-01-01

    Surface functionalization of sensor chip for probe immobilization is crucial for the biosensing applications of surface plasmon resonance (SPR) sensors. In this paper, we report a method circulating the dopamine aqueous solution to coat polydopamine film on sensing surface for surface functionalization of SPR chip. The polydopamine film with available thickness can be easily prepared by controlling the circulation time and the biorecognition elements can be immobilized on the polydopamine fil...

  20. En språkfundert kompetansemodell for planlegging av undervisning

    Directory of Open Access Journals (Sweden)

    Erik Knain

    2016-04-01

    Full Text Available I utdanningsvitenskapelig litteratur er ulike former for ”literacy” et vedvarende fokus både teoretisk og empirisk. I engelskspråklig naturfagdidaktisk litteratur brukes betegnelsen ”scientific literacy”. Jeg skisserer i denne artikkelen et planleggingsverktøy for undervisning som bygger på en eksplisitt teoretisk modell for deltakelse gjennom språk. Modellen er bindeledd mellom et generalisert kompetansebegrep og planlegging av undervisning ved at den knytter sammen de didaktiske spørsmålene hva, hvem, hvordan og hvorfor med en modell for funksjonell deltakelse. Modellen er spesielt relevant for et allmenndannende ”naturfag for alle”, men favner også et naturfag som fokuserer på utdanning av framtidas naturvitere. Artikkelen retter seg mot naturfag i skolen, men modellen bør kunne anvendes også i andre skolefag. Modellen peker mot et situert og transformativt kompetansebegrep.Nøkkelord: naturfag , kompetanse , diskurs , deltakelse, undervisning og læringAbstractVarious forms of literacy have long been the focus of educational discourses, not the least in science education where the term “scientific literacy” has been an enduring concern for decades. In this article I describe a tool for designing teaching based on a theoretical perspective on participation through language. The model connects a general conception of competence with the planning of teaching. To do so it drowe on the didactical questions of “what, who, how and why”. The model is particularly relevant in a “science for all” perspective but also for the educating of future science specialists. Although school science is the primary focus in this article, the model should be applicable to other school subjects as well. The model opens for a situated and transformative notion of competence.Keywords: scientific literacy, discourse, participation, teaching and learning

  1. Prepartion and Characterization of SPR Au Substrate Using Wet Chemical Plating%湿化学镀SPR金基底及其性能表征

    Institute of Scientific and Technical Information of China (English)

    周宥辰; 王艳艳; 马志方; 姜艳霞; 邱瑾; 孙世刚

    2011-01-01

    The present study developed a preparation method of SPR gold substrate based on water phase operation. Firstly, on the glass modified by APTMS we self-assembed a gold colloid monolayer as the catalyst bed;Secondly, using the chemical plating technique, we made the gold film increase equably within the namometer range to obtain the proper shape and thinkness of the gold film required by the SPR. The analysis results of UVvis and TEM demonstrated that the diameter of the monolayer formed from gold nanoparticles was 2.5 nm. Moreover, SEM showed that the nanoparticle prepared by electroless gold plating was uniformly distributed, whose diameter was 40 nm. The SPR flow injection experiment found that the response intensity of the SPR Au substrate made by wet chemical plating was as strong as that of the commercial SPR Au substrate made by vacuum sputtering, and the former one was more preferable to adjust the surface structure of the gold film than the later one.%建立一种由单一水相操作的表面等离子体共振法(SPR)制备金基底.即:在3-氨基丙基三甲氧基硅烷(APTMS)修饰的玻璃片上自组装一层细小的金胶纳米粒子,以此为催化模板,利用化学镀技术在纳米尺度范围内控制金膜的均匀增长,获得优异SPR信号响应所需要的金膜形貌和厚度.紫外光谱(UV-vis),透射电镜(TEM)观测表明,纳米金膜催化模板粒径约为2.5nm.扫描电镜(SEM)观察湿化学镀SPR金基底均匀分布,粒径约为40nm.与商品化真空镀金基底相比,湿化学镀金基底对乙醇的SPR响应强度相当,且可调控性更高.

  2. Analysis of cavern stability at the Bryan Mound SPR site.

    Energy Technology Data Exchange (ETDEWEB)

    Ehgartner, Brian L.; Sobolik, Steven Ronald

    2009-04-01

    This report presents computational analyses that simulate the structural response of caverns at the Strategic Petroleum Reserve Bryan Mound site. The cavern field comprises 20 caverns. Five caverns (1, 2, 4, and 5; 3 was later plugged and abandoned) were acquired from industry and have unusual shapes and a history dating back to 1946. The other 16 caverns (101-116) were leached according to SPR standards in the mid-1980s and have tall cylindrical shapes. The history of the caverns and their shapes are simulated in a 3-D geomechanics model of the site that predicts deformations, strains, and stresses. Future leaching scenarios due to oil drawdowns using fresh water are also simulated by increasing the volume of the caverns. Cavern pressures are varied in the model to capture operational practices in the field. The results of the finite element model are interpreted to provide information on the current and future status of subsidence, well integrity, and cavern stability. The most significant result in this report is relevant to caverns 1, 2, and 5. The caverns have non-cylindrical shapes and have potential regions where the surrounding salt may be damaged during workover procedures. During a workover the normal cavern operating pressure is lowered to service a well. At this point the wellhead pressures are atmospheric. When the workover is complete, the cavern is repressurized. The resulting elastic stresses are sufficient to cause tension and large deviatoric stresses at several locations. With time, these stresses relax to a compressive state due to salt creep. However, the potential for salt damage and fracturing exists. The analyses predict tensile stresses at locations with sharp-edges in the wall geometry, or in the case of cavern 5, in the neck region between the upper and lower lobes of the cavern. The effects do not appear to be large-scale, however, so the only major impact is the potential for stress-induced salt falls in cavern 5, potentially leading to

  3. Ultra-high sensitivity optical biosensor based on Vernier effect in triangular ring resonators (TRRs) with SPR

    Science.gov (United States)

    Kim, Tae-Ryong; Kim, Hong-Seung; Li, Jun; Oh, Geum-Yoon; Kim, Doo-Gun; Choi, Young-Wan

    2015-03-01

    In this paper, surface plasmon resonance triangular ring resonator (SPR-TRR) Vernier structure based on InP is simulated for index variation from 1.33 to 1.35. Sensing area of SPR-TRR is achieved to make an ultra-compact SPR mirror by deposition of Au film layer which is designed to deposit on vertex of TRR. The possibility of mass production is shown by a deposition of SPR mirror on the triangular ring resonator (TRR). Also, the sensitivity enhancement of an envelope signal for Vernier effect is confirmed by FDTD simulation compared to SPR-TRR. As simulation results, the sensitivity is enhanced 20 nm / RIU to 480 nm / RIU. Thus, SPR-TRR Vernier structure is used for a biosensor to enhance the sensitivity of biosensor.

  4. Final state of the Strategic Petroleum Reserve (SPR) Weeks Island Mine

    Energy Technology Data Exchange (ETDEWEB)

    MOLECKE,MARTIN A.

    2000-02-01

    This report documents the decommissioning and abandonment activities at the Weeks Island Strategic Petroleum Reserve (SPR) site, Iberia Parish, Louisiana, that were concluded in 1999. These activities required about six years of intense operational, engineering, geotechnical, and management support efforts, following initiation of site abandonment plans in 1994. The Weeks Island SPR mine stored about 72.5 million bbl of crude oil following oil fill in 1980--1982, until November 1995, when the DOE initiated oil drawdown procedures, with brine refill and oil skimming, and numerous plugging and sealing activities. About 98% of the crude oil was recovered and transferred to other SPR facilities in Louisiana and Texas; a small amount was also sold. This document summarizes recent pre- and post-closure: conditions of surface features at the site, including the sinkholes, the freeze wall, surface subsidence measurements and predictions; conditions within the SPR mine, including oil recovery, brine filling, and the Markel Wet Drift; risk assessment evaluations relevant to the decommissioning and long-term potential environmental impacts; continuing environmental monitoring activities at the site; and, an overview on the background and history of the Weeks Island SPR facility.

  5. Final state of the Strategic Petroleum Reserve (SPR) Weeks Island Mine

    Energy Technology Data Exchange (ETDEWEB)

    MOLECKE,MARTIN A.

    2000-02-01

    This report documents the decommissioning and abandonment activities at the Weeks Island Strategic Petroleum Reserve (SPR) site, Iberia Parish, Louisiana, that were concluded in 1999. These activities required about six years of intense operational, engineering, geotechnical, and management support efforts, following initiation of site abandonment plans in 1994. The Weeks Island SPR mine stored about 72.5 million bbl of crude oil following oil fill in 1980--1982, until November 1995, when the DOE initiated oil drawdown procedures, with brine refill and oil skimming, and numerous plugging and sealing activities. About 98% of the crude oil was recovered and transferred to other SPR facilities in Louisiana and Texas; a small amount was also sold. This document summarizes recent pre- and post-closure: conditions of surface features at the site, including the sinkholes, the freeze wall, surface subsidence measurements and predictions; conditions within the SPR mine, including oil recovery, brine filling, and the Markel Wet Drift; risk assessment evaluations relevant to the decommissioning and long-term potential environmental impacts; continuing environmental monitoring activities at the site; and, an overview on the background and history of the Weeks Island SPR facility.

  6. Surface functionalization of SPR chip for specific molecular interaction analysis under flow condition

    Directory of Open Access Journals (Sweden)

    Tao Ma

    2017-03-01

    Full Text Available Surface functionalization of sensor chip for probe immobilization is crucial for the biosensing applications of surface plasmon resonance (SPR sensors. In this paper, we report a method circulating the dopamine aqueous solution to coat polydopamine film on sensing surface for surface functionalization of SPR chip. The polydopamine film with available thickness can be easily prepared by controlling the circulation time and the biorecognition elements can be immobilized on the polydopamine film for specific molecular interaction analysis. These operations are all performed under flow condition in the fluidic system, and have the advantages of easy implementation, less time consuming, and low cost, because the reagents and devices used in the operations are routinely applied in most laboratories. In this study, the specific absorption between the protein A probe immobilized on the sensing surface and human immunoglobulin G in the buffer is monitored based on this surface functionalization strategy to demonstrated its feasibility for SPR biosensing applications.

  7. SPR based immunosensor for detection of Legionella pneumophila in water samples

    Science.gov (United States)

    Enrico, De Lorenzis; Manera, Maria G.; Montagna, Giovanni; Cimaglia, Fabio; Chiesa, Maurizio; Poltronieri, Palmiro; Santino, Angelo; Rella, Roberto

    2013-05-01

    Detection of legionellae by water sampling is an important factor in epidemiological investigations of Legionnaires' disease and its prevention. To avoid labor-intensive problems with conventional methods, an alternative, highly sensitive and simple method is proposed for detecting L. pneumophila in aqueous samples. A compact Surface Plasmon Resonance (SPR) instrumentation prototype, provided with proper microfluidics tools, is built. The developed immunosensor is capable of dynamically following the binding between antigens and the corresponding antibody molecules immobilized on the SPR sensor surface. A proper immobilization strategy is used in this work that makes use of an important efficient step aimed at the orientation of antibodies onto the sensor surface. The feasibility of the integration of SPR-based biosensing setups with microfluidic technologies, resulting in a low-cost and portable biosensor is demonstrated.

  8. Single chip SPR and fluorescent ELISA assay of prostate specific antigen.

    Science.gov (United States)

    Breault-Turcot, J; Poirier-Richard, H-P; Couture, M; Pelechacz, D; Masson, J-F

    2015-12-01

    A multi-channel system combining fluidics and micropatterned plasmonic materials with wavelength interrogation surface plasmon resonance (SPR) and fluorescence detection was integrated from the combination of a small and motorized fluorescence microscope mounted on a portable 4-channel SPR instrument. The SPR and fluorescent measurements were performed based on the same detection area in a multi-channel fluidic, with a sensing scheme for prostate-specific antigen (PSA) consisting of a sandwich assay with a capture anti-PSA immobilized onto the SPR sensor and a detection anti-PSA modified with horseradish peroxidase (HRP). In this dual-detection instrument, fluorescence was measured from the solution side of the micropatterned gold film, while the interface between the glass prism and the gold film served to interrogate the SPR response. The SPR sensors were comprised of microhole arrays fabricated by photolithography to enhance the instrumental response for PSA detection by approximately a factor of 2 to 3 and they were coated with a self-assembled monolayer of a peptide (3-MPA-HHHDD-OH) to minimize nonspecific adsorption. PSA was successfully detected at clinical concentrations from 10 pM to 50 nM with this integrated system in a single assay lasting 12 minutes, almost centering on the desired range for PSA diagnostic tests (>4 ng mL(-1) or >150 pM). The combination of two robust techniques in a single chip and instrument has led to a simple and effective assay that can be carried out on a small and portable instrument providing rapid biodetection of an important cancer biomarker with a dynamic range of nearly 4 orders of magnitude in the clinical range.

  9. [Monitoring the sewage degradation by analyzing optic fiber SPR spectrum character].

    Science.gov (United States)

    Zhang, Xiao-Li; Liang, Da-Kai; Zeng, Jie; Zhao, Zhi-Yuan; Zeng, Jian-Min

    2010-02-01

    The working principle of the optic fiber SPR sensor was discussed in the present paper at first. The feasibility of using it to monitor the degradation process of the environmental sewage represented by the methyl orange was studied. Finally, the optic fiber SPR sensor was adopted to monitor the change in degradation concentration represented by the original methyl orange solution on the base of 50 mL initial concentration 30 mg x L(-1), and the optic fiber SPR spectrum character of degradation process was analyzed in detail. Meanwhile the UV spectrophotometer was used to measure the change in concentration in the course of the degradation. The measurement data were analyzed and compared at large. The research work indicates that both the methods have consistent results, as the degradation time increases, the absorbance and concentration of the environmental sewage represented by the methyl orange solution decrease by and by, and the resonant wavelength of the optic fiber SPR sensor blue shifts step by step, as compared to the original standardization methyl orange solution resonant spectrum. It was shown that the methyl orange solution was degraded, and the rate of degradation was up to about 73 percent within two hours. The comparative results illustrate that it is feasible to use the optic fiber SPR sensor to monitor the environmental sewage degradation. The research result not only provides a new monitoring method for the degradation process of the environmental sewage, but also promotes the technique of the SPR sensor combined to the environment monitor by a long way.

  10. Monitoring Microalgae Population Growth by using Fe3O4 Nanoparticles-based Surface Plasmon Resonance (SPR) Biosensor

    Science.gov (United States)

    Nurrohman, D. T.; Oktivina, M.; Suharyadi, E.; Suyono, E. A.; Abraha, K.

    2017-05-01

    The population growth of microalgae has been successfully monitored by using the surface plasmon resonance (SPR)-based biosensor activated by using PEG-4000 functionalized Fe3O4 magnetic nanoparticles. The SPR-based biosensor in this work used Kretchmann configuration and Ha-Ne laser beam (λ = 633nm). The population of microalgae were estimated by using calibration curve. The shift of SPR angle happens due to the change of dielectric medium from prism/Au/air system to prism/Au/Fe3O4+PEG+microalgae/cover glass system. The change of dielectric medium occurs due to deposition of Fe3O4+PEG+microalgae samples that were monitored for seven days. The population of microalgae on the first day was estimated of 1188146 cell/ml and SPR angle had shifted from 42.80 to 69.00. The SPR angle shifted as much as 26.20. The population of microalga grew significantly on the third day as much as 1649106 cell/ml and the SPR angle had shifted 43.80 to 71.10. The SPR angle shifted as much as 27.30. On fourth to seventh day, the population of microalgae decreased. The population of microalgae on the seventh day was estimated of 1206961 cell/ml and the SPR angle had shifted from 44.00 to 68.80. The SPR angle shifted as much as 24.8 degrees. The shift of SPR angle was influenced by the population of microalgae so that the SPR-based biosensor has the potential for use in monitoring the microalgae population.

  11. A novel method to prepare SPR sensor chips based on photografting molecularly imprinted polymer

    Institute of Scientific and Technical Information of China (English)

    Qing Quan Wei; Tian Xin Wei

    2011-01-01

    A novel method to prepare surface plasmon resonance (SPR) sensor chips based on grafted imprinted polymer is explored. Benzophenone photografting system is used to grow molecularly imprinted polymer (MIP) films from the modified surface of gold substrate. The surface morphology and thickness of MIP films were investigated by scanning electronic microscope (SEM). The adsorption properties of sensor chip were studied by SPR spectroscopy. The results demonstrate that nano-MIP films can be constructed on the surface of gold substrate with the good adsorption of template molecules.

  12. Ionization of covalent immobilized poly(4-vinylphenol) monolayers measured by ellipsometry, QCM and SPR

    Energy Technology Data Exchange (ETDEWEB)

    Uppalapati, Suji [Department of Chemistry, University of Massachusetts Lowell, 1 University Ave., Lowell, MA 01854 (United States); Kong, Na; Norberg, Oscar [KTH-Royal Institute of Technology, Department of Chemistry, Teknikringen 30, S-10044 Stockholm (Sweden); Ramström, Olof, E-mail: ramstrom@kth.se [KTH-Royal Institute of Technology, Department of Chemistry, Teknikringen 30, S-10044 Stockholm (Sweden); Yan, Mingdi, E-mail: Mingdi_Yan@uml.edu [Department of Chemistry, University of Massachusetts Lowell, 1 University Ave., Lowell, MA 01854 (United States); KTH-Royal Institute of Technology, Department of Chemistry, Teknikringen 30, S-10044 Stockholm (Sweden)

    2015-07-15

    Covalently immobilized poly(4-vinylphenol) (PVP) monolayer films were fabricated by spin coating PVP on perfluorophenyl azide (PFPA)-functionalized surfaces followed by UV irradiation. The pH-responsive behavior of these PVP ultrathin films was evaluated by ellipsometry, quartz crystal microbalance (QCM) and surface plasmon resonance (SPR). By monitoring the responses of these films to pH in situ, the ionization constant of the monolayer thin films was obtained. The apparent pK{sub a} value of these covalently immobilized PVP monolayers, 13.4 by SPR, was 3 units higher than that of the free polymer in aqueous solution.

  13. Interactions of doxorubicin with self-assembled monolayer-modified electrodes: electrochemical, surface plasmon resonance (SPR), and gravimetric studies.

    Science.gov (United States)

    Nieciecka, Dorota; Krysinski, Pawel

    2011-02-01

    We present the results on the partitioning of doxorubicin (DOX), a potent anticancer drug, through the model membrane system, self-assembled monolayers (SAMs) on gold electrodes. The monolayers were formed from alkanethiols of comparable length with different ω-terminal groups facing the aqueous electrolyte: the hydrophobic -CH(3) groups for the case of dodecanethiol SAMs or hydrophilic -OH groups of mercaptoundecanol SAMs. The electrochemical experiments combined with the surface plasmon resonance (SPR) and gravimetric studies show that doxorubicin is likely adsorbed onto the surface of hydrophilic monolayer, while for the case of the hydrophobic one the drug mostly penetrates the monolayer moiety. The adsorption of the drug hinders further penetration of doxorubicin into the monolayer moiety.

  14. Handheld Chem/Biosensor Using Extreme Conformational Changes in Designed Binding Proteins to Enhance Surface Plasmon Resonance (SPR)

    Science.gov (United States)

    2016-04-01

    detection system for chemical and biological toxins . Surface Plasmon Resonance (SPR), protein design, protein engineering, supercharged protein ...chemical and biological toxins . Keywords: Surface Plasmon Resonance (SPR), protein design, protein engineering, supercharged protein , metamaterials...even this small index change, should be capable of detecting larger target molecules, such as proteins or even viral or bacterial pathogens, which

  15. Isobaric tags for relative and absolute quantitation proteomics analysis of gene regulation by SprC in Staphylococcus aureus.

    Science.gov (United States)

    Zhao, Huanqiang; Hu, Fupin; Yang, Han; Ding, Baixing; Xu, Xiaogang; He, Chunyan; Cui, Zelin; Shu, Wen; Liu, Qingzhong

    2017-09-06

    To explore the complete gene networks regulated by small RNA SprC and its targets in Staphylococcus aureus. The isobaric tags for relative and absolute quantitation and bioinformatic methods were utilized to identify and analyze the target proteins affected by SprC in S. aureus N315. Proteomic analysis showed that the expression of 44 proteins was modulated by SprC. Further, bioinformatic analysis displayed that these affected proteins mainly associated with metabolic and cellular process, biological regulation and catalytic activity. Our data provide a rich resource of SprC targets in S. aureus, although the mechanism of regulation by SprC is yet to be elucidated.

  16. DNA Hybridization Detection Based on Resonance Frequency Readout in Graphene on Au SPR Biosensor

    Directory of Open Access Journals (Sweden)

    Md. Biplob Hossain

    2016-01-01

    Full Text Available This paper demonstrates a numerical modeling of surface plasmon resonance (SPR biosensor for detecting DNA hybridization by recording the resonance frequency characteristics (RFC. The proposed sensor is designed based on graphene material as biomolecular recognition elements (BRE and the sharp SPR curve of gold (Au. Numerical analysis shows that the variation of RFC for mismatched DNA strands is quiet negligible whereas that for complementary DNA strands is considerably countable. Here, graphene is used to perform faster immobilization between target DNA and probe DNA. The usage of graphene also changes the RFC that ensure hybridization of DNA event by utilizing its optochemical property. In addition, proposed sensor successfully distinguishes between hybridization and single-nucleotide polymorphisms (SNP by observing the variation level of RFC and maximum transmittance. Therefore, the proposed frequency readout based SPR sensor could potentially open a new window of detection for biomolecular interactions. We also highlight the advantage of using graphene sublayer by performing the sensitivity analysis. Sandwiching of each graphene sublayer enhances 95% sensitivity comparing with conventional SPR sensor.

  17. Preparation of Gd2O2S:Pr Scintillation Ceramics by Pressureless Reaction Sintering Method

    Institute of Scientific and Technical Information of China (English)

    Jingbao Lian; Xudong Sun; Tie Gao; Qiang Li; Xiaodong Li; Zhigang Liu

    2009-01-01

    Fabrication of Gd2O2S:Pr scintillation ceramics by pressureless reaction sintering was investigated. The 2Gd2O3.(Gd,Pr)2(SO4)3.mH2O precursor was made by hydrotherrnal reaction using commercially available Gd2O3, Pr6O11 and H2SO4 as the starting materials. Then single phase Gd2O2SO4:Pr powder was obtained by calcining the precursor at 750℃ for 2 h. The Gd2O2SO4:Pr powder compacts can be sintered to single phase Gd2O2S:Pr ceramics with a relative density of 99% and mean grain size of 30 μm at 1750℃ for 2 h in flowing hydrogen atmosphere. Densification and microstructural development of the Gd2O2S:Pr ceramics were examined. Luminescence spectra of the Gd2O2S:Pr ceramic under 309 nm UV excitation and X-ray excitation show a green emission at 511 nm as the most prominent peak, which corresponds to the 3P0-ZH4 transition of Pr3+ ions.

  18. Surface plasmon resonance (SPR) detection of Staphylococcal Enterotoxin A in food samples

    Science.gov (United States)

    An automated and rapid method for detection of staphylococcal enterotoxins (SE) is needed. A sandwich assay was developed using a surface plasmon resonance (SPR) biosensor for detection of staphylococcal enterotoxin A (SEA) at subpicomolar concentration. Assay conditions were optimized for capturing...

  19. Surface Plasmon Resonance (SPR Phenomenon of the Oxidizing and Reducing Polypyrrole

    Directory of Open Access Journals (Sweden)

    Nurlaila Rajabiah

    2016-12-01

    Full Text Available Surface Plasmon Resonance (SPR phenomenon of the oxidizing and reducing polypyrrole (PPy have been observed using a modified Au/PPy Kretschmann configuration. The observation was carried out through simulation Winspall 3.02 software and compared with some experimental data refractive index (n, absorbance index (, and thickness (τ reported in other literatures with spectroscopy ellipsometry. This simulation assumed that the SPR system use BK-7 halfcylinder prism (n= 1,515 and the laser beam was generated by HeNe (λ= 632,8 nm. The result showed that the optimum layer thickness of polypyrrole with neutral electrolyte solution in the reduction state is of about 20 nm. The polypyrrole with an acid solution in the oxidation state showed that the reflectivity curve is sharper and the width of the curve is smaller than the neutral electrolyte solution in the reduction state with the SPR angle of about 46,810 and the reflectivity value of about 0,217. Polypyrrole in a state of oxidation and reduction, related to the absorption, film thickness, and dielectric constant of materials, affected to the SPR angle shift and dip curve

  20. Binding of leachable components of polymethyl methacrylate (PMMA) and peptide on modified SPR chip

    Science.gov (United States)

    Szaloki, M.; Vitalyos, G.; Harfalvi, J.; Hegedus, Cs

    2013-12-01

    Many types of polymers are often used in dentistry, which may cause allergic reaction, mainly methyl methacrylate allergy due to the leachable, degradable components of polymerized dental products. The aim of this study was to investigate the interaction between the leachable components of PMMA and peptides by Fourier-transform Surface Plasmon Resonance (FT SPR). In our previous work binding of oligopeptides (Ph.D.-7 and Ph.D.-12 Peptide Library Kit) was investigated to PMMA surface by phage display technique. It was found that oligopeptides bounded specifically to PMMA surface. The most common amino acids were leucine and proline inside the amino acids sequences of DNA of phages. The binding of haptens, as formaldehyde and methacrylic acid, to frequent amino acids was to investigate on the modified gold SPR chip. Self assembled monolayer (SAM) modified the surface of gold chip and ensured the specific binding between the haptens and amino acids. It was found that amino acids bounded to modified SPR gold and the haptens bounded to amino acids by creating multilayer on the chip surface. By the application of phage display and SPR modern bioanalytical methods the interaction between allergens and peptides can be investigated.

  1. Detection of mycotoxins using imaging surface plasmon resonance (iSPR)

    Science.gov (United States)

    Significant progress has been made in the development of biosensors that can be used to detect mycotoxins. One technology that has been extensively tested is surface plasmon resonance (SPR). In 2003 a multi-toxin method was reported that detected aflatoxin B1 (AFB1), zearalenone (ZEA), fumonisin B1 ...

  2. Comparison of sensing strategies in SPR biosensor for rapid and sensitive enumeration of bacteria.

    Science.gov (United States)

    Torun, Ozlem; Hakkı Boyacı, Ismail; Temür, Erhan; Tamer, Uğur

    2012-01-01

    Rapid and sensitive detections of microorganisms are very important for biodefence, food safety, medical diagnosis and pharmaceutics. The present study aims to find out the most proper bioactive surface preparation method to develop rapid, sensitive and selective bacteria biosensor, based on surface plasmon resonance (SPR) spectroscopy. Escherichia coli (E. coli) was used as a model bacterium and four sensing strategies in SPR were tested. Three of these strategies are antibody immobilization methods that are non-specific adsorption, specific adsorption via the avidin-biotin interaction, and immobilization of antibodies via self-assembled monolayer formation. The fourth strategy is a novel method for bacteria enumeration based on the combination of the SPR spectroscopy and immunomagnetic separation with using gold-coated magnetic nanoparticles. According to results, the most efficient SPR method is the one based on gold-coated magnetic nanoparticles. This method allows to specifically separate E. coli from the environment and to quantify rapidly without any labeling procedure. The developed method has a linear range between 30 and 3.0 × 10(4)cfu/ml, and a detection limit of 3 cfu/ml. The selectivity of the method was examined with Enterobacter aerogenes and Enterobacter dissolvens, which did not produce any significant response. The usefulness of the method to detect E. coli in real water samples was also investigated, and the results were compared with the results from plate-counting method. There was no significant difference between the methods (p>0.05).

  3. A computer model for the prediction of sensitivity in SPR sensing platforms

    Science.gov (United States)

    Izquierdo, Kristel D.; Salazar, Arnoldo; Losoya-Leal, Adrian; Martinez-Chapa, Sergio O.

    2015-03-01

    Surface Plasmon Resonance (SPR) is a wave phenomenon occurring at a metal-dielectric interface. A SPR-based biosensor operates by monitoring changes in the refractive index close to the interface that are produced in response to the interaction between the analyte and the receptors immobilized on the metal's surface. The performance of these sensors depends on many parameters, including channel geometry, material properties and parameters related to chemical interaction between the analyte and immobilized receptors. This paper presents an integrated model that predicts the sensitivity of an SPR-based sensing platform under the Kretschmann configuration. The model uses the analytical solution of the differential equations that describe the analyte-bioreceptor interaction to correlate changes in analyte concentration to changes in refractive index at the sensing surface. These results are then connected with COMSOL simulations that relate changes in refractive index to changes in the SPR reflectivity curves. The resultant relations are integrated and the model is evaluated under different scenarios. This model will aid in the optimization of assay parameters prior to experimentation for maximum sensitivity; saving both time and expensive chemical reagents during the experimental phase.

  4. Potential of SPR sensors based on multilayer interfaces with gold and LHM for biosensing applications

    Science.gov (United States)

    Cherifi, Abdellatif; Bouhafs, Benamar

    2017-09-01

    Recently, the subject on "plasmonics" has received significant attention in designing surface plasmon resonance (SPR) sensors. In order to achieve extremely high-sensitivity sensing, multilayered configurations based on a variety of active materials and dielectrics have been exploited. In this work, a novel SPR sensor is proposed and investigated theoretically. The structure, analyzed in attenuated total reflection (ATR), consists of multilayer interfaces between gold and a metamaterial (LHM) separated by an analyte layer as a sensing medium. By interchanging between gold and LHM, under the effect of the refractive index (RI) of analyte set to be in the range of 1.00 to 1.99, the sharp peak reflectivity at the SPR angle takes two opposite behaviors predicted from the transfer matrix method. At the threshold value of 1.568 of the refractive index of analyte and when the LHM is the outer medium, the layered structure exhibits a giant sharp peak located at 43° of intensity up to 105 due to the Goos-Hànchen effect. With respect to the refractive index (RI) change and thickness of analyte, the characteristics (intensity, resonance condition, and quality factor) of the SPR mode, which make the proposed device have the potential for biosensing applications, have been analytically modelized.

  5. Near-infrared grating-assisted SPR optical fiber sensors: design rules for ultimate refractometric sensitivity.

    Science.gov (United States)

    Caucheteur, Christophe; Voisin, Valérie; Albert, Jacques

    2015-02-09

    Plasmonic optical fiber sensors are continuously developed for (bio)chemical sensing purposes. Recently, surface plasmon resonance (SPR) generation was achieved in gold-coated tilted fiber Bragg gratings (TFBGs). These sensors probe the surrounding medium with near-infrared narrowband resonances, which enhances both the penetration depth of the evanescent field in the external medium and the wavelength resolution of the interrogation. They constitute a unique configuration to probe all the fiber cladding modes individually. We use them to analyze the modal distribution of gold-coated telecommunication-grade optical fibers immersed in aqueous solutions. Theoretical investigations with a finite-difference complex mode solver are confirmed by experimental data obtained on TFBGs. We show that the refractometric sensitivity varies with the mode order and that the global SPR envelope shift in response to surrounding refractive index (SRI) changes higher than 1e-2 RIU (refractive index unit) can be ~25% bigger than the local SPR mode shift arising from SRI changes limited to 1e-4 RIU. We bring clear evidence that the optimum gold thickness for SPR generation lies in the range between 50 and 70 nm while a cladding diameter decrease from 125 µm to 80 µm enhances the refractometric sensitivity by ~20%. Finally, we demonstrate that the ultimate refractometric sensitivity of cladding modes is ~550 nm/RIU when they are probed by gold-coated TFBGs.

  6. New insights into the mucoadhesion of pectins by AFM roughness parameters in combination with SPR

    DEFF Research Database (Denmark)

    Joergensen, Lars; Klösgen, Beate; Simonsen, Adam Cohen;

    2011-01-01

    The object of this study was to assess the mucoadhesion of the three main commercially available types of pectin by atomic force microscopy (AFM) and surface Plasmon resonance (SPR). Polyacrylic acid and polyvinyl pyrrolidone were used as positive and negative control, respectively. Image analysis...

  7. Detection of phenols in potable water using SPR and specific receptors

    Energy Technology Data Exchange (ETDEWEB)

    Oliver, Jennifer Valerie

    2002-07-01

    The potential of thin films of a range of molecular receptors for surface plasmon resonance (SPR) sensing of a range of phenolic molecules in water has been explored. Receptors included molecular clips based on diarylglycouril units with two aromatic 'walls'; and polyalkylviologen (PAV) and cyclobis(paraquat-p-phenylene) (CPP) dications, two examples with polystyrene sulphonate (PSS) counterions. Films containing sulphonated polycyclodextrins were also evaluated. Target phenols included phenol, three benzene diols and two triols, three hydroxylated biphenyls and a range of related molecules. Receptor films were spin-coated, at up to 10,000rpm, as thin films (<200nm) onto glass slides coated with 48nm of silver. Effects of aqueous analytes on the position and profile of their SPR were examined by goniometer scanning and fixed angle measurement of intensity changes using a 632.8nm laser. A novel CCD array detector, utilising LED narrow band illumination peaking at 654nm, later yielded whole curve, real-time observations. Fixed-angle SPR responses of all viologen film types tested varied in direction, size and rate for different phenols, and depended on both the concentration of the phenol and on the age of its aqueous solution, permitting detection down to 10ppm. GC/MS studies confirmed the presence of polyphenols as oxidatively coupled phenolic breakdown products in the aged solutions. Greater sensitivity to polyphenols than to the original monomers was observed. The mechanistic basis of these observations is discussed with reference to SPR responses of the receptors to a series of model compounds designed to clarify the relative roles of hydrogen bonding, charge transfer and {pi}-{pi} interactions. Theoretical modelling of the responses has also been undertaken. Based on these results, time-dependent SPR response patterns for films of three different receptors were shown to permit unambiguous identification of simple phenols in water. The potential of

  8. Development of SPR-6 Pipe Handling Device%SPR-6钻柱自动排放装置的研制

    Institute of Scientific and Technical Information of China (English)

    高迅; 徐军; 彭太锋; 李勇

    2016-01-01

    The traditional drilling rig needs high-altitude operations by derrickman on the monkey board in drill string racking and transporting, presenting high risk, intensive labor and low efficiency. In view of this, the SPR-6 pipe handling device has been developed. The device mainly consists of the upper grab arm, the lower grab arm and electro-hydraulic control system. Through centralized control, the upper and the lower grab arms achieve synchronous interaction to grab, move and rack the drill string, so to achieve automatic drill string racking. The testing results showed that, the SPR-6 automatic pipe handling device has a rational structure design, high accu-racy, and operation flexibility, stable and reliable running, thus achieving automatic and unmanned pipe han-dling, reduced labor intensity and improved drilling safety.%传统石油钻机在排放和输送钻柱作业过程中需要井架工在二层台从事高空作业,危险系数高,劳动强度大,作业效率低.鉴于此,研制了SPR-6钻柱自动排放装置.该装置主要由上部机械手、 下部机械手和电液控制系统等部分组成,通过集中控制,实现上部机械手和下部机械手的同步联动,达到抓取、 移动和排放钻柱的目的.试验结果表明,SPR-6钻柱自动排放装置结构设计合理,定位精度高,操作灵活,运行平稳可靠,减轻了工人的劳动强度,提高了钻井作业安全性.该装置实现了钻柱排放作业自动化和无人化操作.

  9. A Novel Method for Heightening Sensitivity of Prism Coupler-Based SPR Sensor

    Institute of Scientific and Technical Information of China (English)

    ZHANG Zhi-Wei; WEN Ting-Dun; WU Zhi-Fang

    2011-01-01

    We present a novel method for heightening the sensitivity of a prism coupler-based surface plasmon resonance (SPR) sensor. The method is based on the total reflection prism made of BK7 glass combined with the Kretschmann geometry of theattenuated total reflection (ATR) method. Compared to the conventional methods of prism coupler-based SPR the novel method provides higher semsitivity to the measurement system.Theoretical simulations show that the detetion sensitivity to the refractive index (RI) of the sensor based on the novel approach has a strong dependence on the thickness of the metal layer. The RI resolution of the sensor is predicted to be 8 × 107 refractive indox units (RIU) under the condition of optimum metal film thickness.This novel method can leave out a precision angle rotation device in the angle modulation and it is unnecessary to adjust the acceptance angle of the light detector. The principal advantage of this method over other methods of light intensity modulation based on prism coupler-based SPR is high sensitivity, expediency to measure and aplication of long distances.%@@ We present a novel method for heightening the sensitivity of a prism coupler-based surface plasmon resonance(SPR) sensor.The method is based on the total reflection prism made of BK7 glass combined with the Kretschmann geometry of theattenuated total reflection(ATR) method.Compared to the conventional methods of prism coupler-based SPR,the novel method provides higher sensitivity to the measurement system.Theoretical simulations show that the detection sensitivity to the refractive index(RI) of the sensor based on the novel approach has a strong dependence on the thickness of the metal layer.The RI resolution of the sensor is predicted to be 8 × 10-7 refractive index units(RIU) under the condition of optimum metal film thickness.This novel method can leave out a precision angle rotation device in the angle modulation and it is unnecessary to adjust the acceptance angle of

  10. SPR optimization using metamaterials in a D-type PCF refractive index sensor

    Science.gov (United States)

    Santos, D. F.; Guerreiro, A.; Baptista, J. M.

    2017-01-01

    Using the finite element method (FEM), this paper presents a numerical investigation of the performance analysis of a D-type photonic crystal fiber (D-type PCF) for refractive index sensing, based on surface plasmon resonance (SPR) with a planar structure made out of a metamaterial. COMSOL Multiphysics was used to evaluate the design of the referred refractive index optical fiber sensor, with higher accuracy and considerable economy of time and resources. A study of different metamaterials concentrations conformed by aluminum oxide (Al2O3) and silver (Ag) is carried out. Another structural parameters, which influences the refractive index sensor performance, the thickness of the metamaterial, is also investigated. The results indicate that the use of metamaterials provides a way of improving the performance of SPR sensors on optical fibers and allows to tailor the working parameters of the sensor.

  11. Fra det spontane til det formelle – å språksette kunnskap

    DEFF Research Database (Denmark)

    Alrø, Helle; Johnsen- Høines, Marit

    2013-01-01

    Dette kapittelet har fokus på elevers læring i matematikk, og deres evne til å kommunisere kunnskapene sine muntlig og skriftlig. Studenter, faglærere og praksislærere samtaler om elevers språkliggjøring av kunnskap, og om hvordan forskjellige måter å uttrykke kunnskaper på kan sies å ha forskjel...

  12. Development of diagnostic SPR based biosensor for the detection of pharmaceutical compounds in saliva

    Science.gov (United States)

    Sonny, Susanna; Sesay, Adama M.; Virtanen, Vesa

    2010-11-01

    The aim of the study is to develop diagnostic tests for the detection of pharmaceutical compounds in saliva. Oral fluid is increasingly being considered as an ideal sample matrix. It can be collected non-invasively and causes less stress to the person being tested. The detection of pharmaceutical compounds and drugs in saliva can give valuable information on individual bases on dose response, usage, characterization and clinical diagnostics. Surface plasmon resonance (SPR) is a highly sensitive, fast and label free analytical technique for the detection of molecular interactions. The specific binding of measured analyte onto the active gold sensing surface of the SPR device induces a refractive index change that can be monitored. To monitor these pharmaceutical compounds in saliva the immunoassays were developed using a SPR instrument. The instrument is equipped with a 670nm laser diode and has two sensing channels. Monoclonal antibodies against the pharmaceutical compounds were used to specifically recognise and capture the compounds which intern will have an effect of the refractive index monitored. Preliminary results show that the immunoassays for cocaine and MDMA (3,4-methylenedioxymethamphetamine) are very sensitive and have linear ranges of 0.01 pg/ml - 1 ng/ml and 0.1 pg/ml - 100 ng/ml, respectively.

  13. Transmission-type SPR sensor based on coupling of surface plasmons to radiation modes using a dielectric grating

    Institute of Scientific and Technical Information of China (English)

    Changkui HU; Deming LIU

    2009-01-01

    A transmission-type surface plasmon resonance (SPR) sensor is presented. In the transmission-type SPR structure, surface plasmon waves are outcoupled to radia-tion modes by the use of dielectric grating on a thin-film layer of Ag. Compared with the traditional reflection-type SPR sensor, the new method provides larger detectable range, which might be useful to investigate thick targets such as in cell analysis. Theoretical simulations show that the structures provide high transmission efficiency for surface plasmon resonance and the devices present extre-mely linear sensing characteristics. Furthermore, it is found that the transmission efficiency and the refractive index detection sensitivity of the SPR sensor can be improved by the use of a lower refractive index glass prism.

  14. Vurdering af omfang og konsekvenser af sprøjtning og gødskning af § 3-beskyttede naturarealer

    DEFF Research Database (Denmark)

    Strandberg, Morten Tune; Bak, Jesper Leth; Bladt, Jesper Stentoft;

    2012-01-01

    Det er overvejende de ferske enge, der gødskes og sprøjtes, hvorimod dette kun undtagelsesvist er konstateret på andre naturtyper som overdrev og heder m.m. De ferske enge er samtidig den mest udbredte § 3-naturtype. Behandling i form af gødskning og sprøjtning foregår anslået på op til 20 % af a...

  15. Effect of Fe3O4 Magnetic Nanoparticle Concentration on the Signal of Surface Plasmon Resonance (SPR) Spectroscopy

    Science.gov (United States)

    Oktivina, M.; Nurrohman, D. T.; Rinto, A. N. Q. Z.; Suharyadi, E.; Abraha, K.

    2017-05-01

    Effect of Fe3O4 magnetic nanoparticle concentration on the signal of surface plasmon resonance (SPR) spectra has been successfully observed. The Fe3O4 nanoparticles with a particle size of about 10.5 ± 0.2 nm were used as active materials to increase the SPR response. X-ray diffraction (XRD) pattern showed that Fe3O4 nanoparticles have a high degree of crystallinity with spinel structure. The SPR system was successfully set up by using a glass prism coupler in a Kretschmann configuration in which gold (Au) thin film was thermally evaporated on the prism base. A green laser of wavelength 543 nm was used as light source. The angular scan in the attenuated total reflection (ATR) spectra showed a dropping intensity. Those things clearly indicated the appearance of SPR coupling phenomenon on the interface of Au thin film. The SPR spectra of fixed Au masses were also performed with same angular positions of dips. The Fe3O4 nanoparticles were deposited on gold thin film as a third layer which was synthesized via co-precipitation method. Hence, it was observed that the variation of Fe3O4 concentration affected the SPR spectra profile. The concentrations of Fe3O4 nanoparticles are 1, 3, 5, 7, 9, and 11 mg/ml which correspond to the angle shift of 0.1°, 0.3°, 0.5°, 0.7°, 0.9°, and 1.0°, respectively. The SPR angle of the dip was shifted to higher value due to change of refractive index of the medium as Fe3O4 nanoparticles concentration increases. Based on this result, we can conclude that the angle shift of SPR increases with increasing concentration of Fe3O4 nanoparticles.

  16. The microtubule plus-end tracking proteins SPR1 and EB1b interact to maintain polar cell elongation and directional organ growth in Arabidopsis.

    Science.gov (United States)

    Galva, Charitha; Kirik, Viktor; Lindeboom, Jelmer J; Kaloriti, Despoina; Rancour, David M; Hussey, Patrick J; Bednarek, Sebastian Y; Ehrhardt, David W; Sedbrook, John C

    2014-11-01

    The microtubule plus-end tracking proteins (+TIPs) END BINDING1b (EB1b) and SPIRAL1 (SPR1) are required for normal cell expansion and organ growth. EB proteins are viewed as central regulators of +TIPs and cell polarity in animals; SPR1 homologs are specific to plants. To explore if EB1b and SPR1 fundamentally function together, we combined genetic, biochemical, and cell imaging approaches in Arabidopsis thaliana. We found that eb1b-2 spr1-6 double mutant roots exhibit substantially more severe polar expansion defects than either single mutant, undergoing right-looping growth and severe axial twisting instead of waving on tilted hard-agar surfaces. Protein interaction assays revealed that EB1b and SPR1 bind each other and tubulin heterodimers, which is suggestive of a microtubule loading mechanism. EB1b and SPR1 show antagonistic association with microtubules in vitro. Surprisingly, our combined analyses revealed that SPR1 can load onto microtubules and function independently of EB1 proteins, setting SPR1 apart from most studied +TIPs in animals and fungi. Moreover, we found that the severity of defects in microtubule dynamics in spr1 eb1b mutant hypocotyl cells correlated well with the severity of growth defects. These data indicate that SPR1 and EB1b have complex interactions as they load onto microtubule plus ends and direct polar cell expansion and organ growth in response to directional cues.

  17. The Microtubule Plus-End Tracking Proteins SPR1 and EB1b Interact to Maintain Polar Cell Elongation and Directional Organ Growth in Arabidopsis[W

    Science.gov (United States)

    Galva, Charitha; Kirik, Viktor; Lindeboom, Jelmer J.; Kaloriti, Despoina; Rancour, David M.; Hussey, Patrick J.; Bednarek, Sebastian Y.; Ehrhardt, David W.; Sedbrook, John C.

    2014-01-01

    The microtubule plus-end tracking proteins (+TIPs) END BINDING1b (EB1b) and SPIRAL1 (SPR1) are required for normal cell expansion and organ growth. EB proteins are viewed as central regulators of +TIPs and cell polarity in animals; SPR1 homologs are specific to plants. To explore if EB1b and SPR1 fundamentally function together, we combined genetic, biochemical, and cell imaging approaches in Arabidopsis thaliana. We found that eb1b-2 spr1-6 double mutant roots exhibit substantially more severe polar expansion defects than either single mutant, undergoing right-looping growth and severe axial twisting instead of waving on tilted hard-agar surfaces. Protein interaction assays revealed that EB1b and SPR1 bind each other and tubulin heterodimers, which is suggestive of a microtubule loading mechanism. EB1b and SPR1 show antagonistic association with microtubules in vitro. Surprisingly, our combined analyses revealed that SPR1 can load onto microtubules and function independently of EB1 proteins, setting SPR1 apart from most studied +TIPs in animals and fungi. Moreover, we found that the severity of defects in microtubule dynamics in spr1 eb1b mutant hypocotyl cells correlated well with the severity of growth defects. These data indicate that SPR1 and EB1b have complex interactions as they load onto microtubule plus ends and direct polar cell expansion and organ growth in response to directional cues. PMID:25415978

  18. Controlled nucleation and growth of surface-confined gold nanoparticles on a (3-aminopropyl)trimethoxysilane-modified glass slide: a strategy for SPR substrates.

    Science.gov (United States)

    Jin, Y; Kang, X; Song, Y; Zhang, B; Cheng, G; Dong, S

    2001-07-01

    The thickness of the gold film and its morphology, including the surface roughness, are very important for getting a good, reproducible response in the SPR technique. Here, we report a novel alternative approach for preparing SPR-active substrates that is completely solution-based. Our strategy is based on self-assembly of the gold colloid monolayer on a (3-aminopropyl)trimethoxysilane-modified glass slide, followed by electroless gold plating. Using this method, the thickness of films can be easily controlled at the nanometer scale by setting the plating time in the same conditions. Surface roughness and morphology of gold films can be modified by both tuning the size of gold nanoparticles and agitation during the plating. Surface evolution of the Au film was followed in real time by UV-vis spectroscopy and in situ SPRS. To assess the surface roughness and electrochemical stability of the Au films, atomic force microscopy and cyclic voltammetry were used. In addition, the stability of the gold adhesion is demonstrated by three methods. The as-prepared Au films on substrates are reproducible and stable, which allows them to be used as electrodes for electrochemical experiments and as platforms for studying SAMs.

  19. SPR sensors in POF: a new experimental configuration for extended refractive index range and better SNR

    Science.gov (United States)

    Cennamo, N.; Coelho, L.; Guerreiro, A.; Jorge, P. A. S.; Zeni, L.

    2014-05-01

    In this work we present a new low cost SPR (Surface Plasmon Resonance) sensor configuration based on efficient higher-order mode filtering in plastic multimode fibers, using a tapered POF (Plastic Optical Fiber) after the sensor system, without decreasing the sensitivity of the sensor. In particular, we present the experimental results obtained with this new configuration. The experimental results have shown as the tapered POF after the sensor system influences the performances in terms of refractive index range and Signal-to-Noise Ratio (SNR).

  20. A SPR aptasensor for detection of avian influenza virus H5N1.

    Science.gov (United States)

    Bai, Hua; Wang, Ronghui; Hargis, Billy; Lu, Huaguang; Li, Yanbin

    2012-01-01

    Rapid and specific detection of avian influenza virus (AIV) is urgently needed due to the concerns over the potential outbreaks of highly pathogenic H5N1 influenza in animals and humans. Aptamers are artificial oligonucleic acids that can bind specific target molecules, and show comparable affinity for target viruses and better thermal stability than monoclonal antibodies. The objective of this research was to use a DNA-aptamer as the specific recognition element in a portable Surface Plasmon Resonance (SPR) biosensor for rapid detection of AIV H5N1 in poultry swab samples. A SPR biosensor was fabricated using selected aptamers that were biotinylated and then immobilized on the sensor gold surface coated with streptavidin via streptavidin-biotin binding. The immobilized aptamers captured AIV H5N1 in a sample solution, which caused an increase in the refraction index (RI). After optimizing the streptavidin and aptamer parameters, the results showed that the RI value was linearly related (R(2) = 0.99) to the concentration of AIV in the range of 0.128 to 1.28 HAU. Negligible signal (H5N1) was observed from six non-target AIV subtypes. The AIV H5N1 in poultry swab samples with concentrations of 0.128 to 12.8 HAU could be detected using this aptasensor in 1.5 h.

  1. A SPR Aptasensor for Detection of Avian Influenza Virus H5N1

    Directory of Open Access Journals (Sweden)

    Huaguang Lu

    2012-09-01

    Full Text Available Rapid and specific detection of avian influenza virus (AIV is urgently needed due to the concerns over the potential outbreaks of highly pathogenic H5N1 influenza in animals and humans. Aptamers are artificial oligonucleic acids that can bind specific target molecules, and show comparable affinity for target viruses and better thermal stability than monoclonal antibodies. The objective of this research was to use a DNA-aptamer as the specific recognition element in a portable Surface Plasmon Resonance (SPR biosensor for rapid detection of AIV H5N1 in poultry swab samples. A SPR biosensor was fabricated using selected aptamers that were biotinylated and then immobilized on the sensor gold surface coated with streptavidin via streptavidin-biotin binding. The immobilized aptamers captured AIV H5N1 in a sample solution, which caused an increase in the refraction index (RI. After optimizing the streptavidin and aptamer parameters, the results showed that the RI value was linearly related (R2 = 0.99 to the concentration of AIV in the range of 0.128 to 1.28 HAU. Negligible signal ( < 4% of H5N1 was observed from six non-target AIV subtypes. The AIV H5N1 in poultry swab samples with concentrations of 0.128 to 12.8 HAU could be detected using this aptasensor in 1.5 h.

  2. Dynamic SPR monitoring of yeast nuclear protein binding to a cis-regulatory element.

    Science.gov (United States)

    Mao, Grace; Brody, James P

    2007-11-09

    Gene expression is controlled by protein complexes binding to short specific sequences of DNA, called cis-regulatory elements. Expression of most eukaryotic genes is controlled by dozens of these elements. Comprehensive identification and monitoring of these elements is a major goal of genomics. In pursuit of this goal, we are developing a surface plasmon resonance (SPR) based assay to identify and monitor cis-regulatory elements. To test whether we could reliably monitor protein binding to a regulatory element, we immobilized a 16bp region of Saccharomyces cerevisiae chromosome 5 onto a gold surface. This 16bp region of DNA is known to bind several proteins and thought to control expression of the gene RNR1, which varies through the cell cycle. We synchronized yeast cell cultures, and then sampled these cultures at a regular interval. These samples were processed to purify nuclear lysate, which was then exposed to the sensor. We found that nuclear protein binds this particular element of DNA at a significantly higher rate (as compared to unsynchronized cells) during G1 phase. Other time points show levels of DNA-nuclear protein binding similar to the unsynchronized control. We also measured the apparent association complex of the binding to be 0.014s(-1). We conclude that (1) SPR-based assays can monitor DNA-nuclear protein binding and that (2) for this particular cis-regulatory element, maximum DNA-nuclear protein binding occurs during G1 phase.

  3. LUSH-based SPR sensor for the detection of alcohols and pheromone

    Science.gov (United States)

    Lau, Hui-Chong; Lee, Yeon-Kyung; Kwon, Jae-Young; Sohn, Young-Soo; Lim, Jeong Ok

    2013-05-01

    Protein is a widely used sensing substrate in the biosensing technology. In the study conducted here, we used odorant binding protein, LUSH from Drosophila as a biosensing substrate in a miniaturized surface plasmon resonance (SPR) sensor. LUSH contains the specific alcohols binding sites, which mediates the detection of alcohols and pheromone. We first modified the surface of the gold sensor chip using the self assembled monolayer in the chloroform solution. The saturated concentration was determined prior to the detection of alcohols and pheromone at various concentrations. The results showed that the LUSH was saturated at 1000 μg/ml on the gold sensor chip. The detection response of LUSH was significant at higher concentration of alcohols. LUSH detected ethanol at concentration >=50% propanol was detected at >=25% whereas pheromone was detected at >=1.25 μg/μl. The results provide some fundamental information on the potential use of LUSH-based SPR as a simple and easy protein-based sensor in the near future.

  4. SPR platform based on image acquisition for HER2 antigen detection

    Science.gov (United States)

    Monteiro, Johny P.; Predabon, Sheila M.; Bonafé, Elton G.; Martins, Alessandro F.; Brolo, Alexandre G.; Radovanovic, Eduardo; Girotto, Emerson M.

    2017-01-01

    HER2 antigen is a marker used for breast cancer diagnosis and prevention. Its determination has great importance since breast cancer is one of the most insidious types of cancer in women. HER2 antigen assessment in human serum is traditionally achieved by enzyme-linked immunosorbent assay (ELISA method), but it has some disadvantages, such as suppressing the thermodynamic-kinetic studies regarding the antibody-antigen interaction, and the use of labeled molecules that can promote false positive responses. Biosensors based on surface plasmon resonance (SPR) are sensitive optical techniques widely applied on bioassays. The plasmonic devices do not operate with labeled molecules, overcoming conventional immunoassay limitations, and enabling a direct detection of target analytes. In this way, a new SPR biosensor to assess HER2 antigen has been proposed, using nanohole arrays on a gold thin film by signal transduction of transmitted light measurements from array image acquisitions. These metallic nanostructures may couple the light directly on surface plasmons using a simple collinear arrangement. The proposed device reached an average sensitivity for refractive index (RI) variation on a metal surface of 4146 intensity units/RIU (RIU = RI units). The device feasibility on biomolecular assessment was evaluated. For this, 3 ng ml-1 known HER2 antigen concentration was efficiently flowed (using a microfluidic system) and detected from aqueous solutions. This outcome shows that the device may be a powerful apparatus for bioassays, particularly toward breast cancer diagnosis and prognosis.

  5. Chemical library screening using a SPR-based inhibition in solution assay: simulations and experimental validation.

    Science.gov (United States)

    Choulier, Laurence; Nominé, Yves; Zeder-Lutz, Gabrielle; Charbonnier, Sebastian; Didier, Bruno; Jung, Marie-Louise; Altschuh, Danièle

    2013-09-17

    We have developed a surface plasmon resonance (SPR)-based inhibition in solution assay (ISA) to search for inhibitors of the medium affinity (KD = 0.8 μM) interaction between an E6-derived peptide (E6peptide) immobilized on the sensor and a PDZ domain (MAGI-1 PDZ1) in the mobile phase. DZ domains are widespread protein-protein interaction modules that recognize the C-terminus of various partners. Simulations indicated that relatively low compound concentrations (10 μM) and limited peptide densities (Rmax < 200 resonance units) should allow the detection of inhibitors with a target affinity close to 100 μM, which was then demonstrated experimentally. ISA screening, carried out on the Prestwick Chemical Library® (1120 compounds), identified 36 compounds that inhibited the interaction by more than 5%. Concentration-dependent ISA, carried out on a subset of 19 potential inhibitors, indicated that 13 of these indeed affected the interaction between MAGI-1 PDZ1 and the E6peptide. No effect was observed for 84 compounds randomly chosen among noninhibitors. One of the four best inhibitors was a peptide binder, and three were PDZ binders with KD in the 10-50 μM range. We propose that a medium (μM) affinity between the target and surface-bound partner is optimal for SPR-based ISA screening.

  6. Urinary micro-RNA biomarker detection using capped gold nanoslit SPR in a microfluidic chip.

    Science.gov (United States)

    Mousavi, Mansoureh Z; Chen, Huai-Yi; Lee, Kuang-Li; Lin, Heng; Chen, Hsi-Hsien; Lin, Yuh-Feng; Wong, Chung-Shun; Li, Hsiao Fen; Wei, Pei-Kuen; Cheng, Ji-Yen

    2015-06-21

    Successful diagnosis and treatment of many diseases depends on the availability of sensitive, reliable and low cost tools for the detection of the biomarkers associated with the diseases. Simple methods that use non-invasive biological samples are especially suitable for the deployment in the clinical environment. In this paper we demonstrate the application of a method that employs a capped gold nanoslit surface plasmon resonance (SPR) sensor and a microfluidic chip for the detection of a urinary nucleic acid biomarker in clinical samples. This method detects low concentrations of the biomarker in a relatively large volume (∼1 mL) of the sample. The method utilizes magnetic nanoparticles (MNPs) for the isolation of target molecules and signal enhancement in conjunction with surface plasmon resonance (SPR) on capped gold nanoslits. The ability of the method to detect urinary miRNA-16-5p in AKI patients was tested and the result was compared with the data obtained with the polymerase chain reaction (PCR). miRNA-16-5p has been found to be a specific and noninvasive biomarker for acute kidney injury (AKI). Our method allows the detection of the biomarker in the urine of AKI patients without amplification and labeling of the target molecules.

  7. A semi-automated AFM photomask repair process for manufacturing application using SPR6300

    Science.gov (United States)

    Dellagiovanna, Mario; Yoshioka, Hidenori; Miyashita, Hiroyuki; Murai, Shiaki; Nakaue, Takuya; Takaoka, Osamu; Uemoto, Atsushi; Kikuchi, Syuichi; Hagiwara, Ryoji; Benard, Stephane

    2007-10-01

    For almost a decade Nanomachining application has been studied and developed to repair next generation of photomasks. This technique, based on Atomic Force Microscopy (AFM), applies a mechanical removing of the defects with almost negligible quartz-damage, high accuracy of the edge-placement and without spurious depositions (stain, implanted elements, etc.) that may affect the optical transmission. SII NanoTechnology Inc. (SIINT) is carrying out a joint-development project with DNP Photomask Europe S.p.A. (DPE) that has allowed the installation in DPE of the next generation state-of-the-art AFM based system SPR6300 to meet the repair specifications for the 65 nm Node. Drift phenomena of the AFM probe represent one of the major obstacles for whichever kind of nano-manipulation (imaging and material or pattern modification). AFM drift undermines the repeatability and accuracy performances of the process. The repair methodology, called NewDLock, implemented on SPR6300, is a semi-automated procedure by which the drift amount, regardless of its origin, is estimated in advance and compensated during the process. Now AFM Nanomachining approach is going to reveal properties of repeatability and user-friendly utilization that make it suitable for the production environment.

  8. A polydopamine-modified optical fiber SPR biosensor using electroless-plated gold films for immunoassays.

    Science.gov (United States)

    Shi, Se; Wang, Libing; Su, Rongxin; Liu, Boshi; Huang, Renliang; Qi, Wei; He, Zhimin

    2015-12-15

    A sensitive and stable electroless-plated gold film for the preparation of an optical fiber surface plasmon resonance (SPR) sensor is presented in this work, together with a facile antibody immobilization method. Gold nanoparticles were uniformly adsorbed onto the surface of an optical fiber forming a film with a thickness of approximately 56.3 nm. The sensor had a high sensitivity with 2054 nm/RIU and 3980 nm/RIU in the refractive index ranges of 1.333-1.359 and 1.359-1.386, respectively. An SPR biosensor was developed based on polydopamine-modified gold film (PDA-Au), which was fabricated by a simple and quick spontaneous polymerization of dopamine (DA) on the gold film. When goat anti-human IgG antibodies were immobilized, the PDA-Au surface had a larger resonant wavelength shift of 66.21 nm compared with the traditional 11-mercaptoundecanoic acid-modified gold film (MUA-Au) surface. In addition, the PDA-Au surface enabled the sensitive and selective determination of human IgG down to a concentration of 2 μg mL(-1) with a high sensitivity of 0.41 nm per μg mL(-1). The PDA-Au surface exhibited an approximately four fold higher sensitivity and an about seven fold lower LOD than the MUA-Au surface to human IgG.

  9. Stability Modification of SPR Silver Nano-Chips by Alkaline Condensation of Aminopropyltriethoxysilane

    Directory of Open Access Journals (Sweden)

    M. Ghorbanpour

    2015-04-01

    Full Text Available The Silver SPR chip was modified by alkaline-silane condensation with aminopropyltriethoxysilane (APTES in NaOH aqueous solution at different times. Silver sputtered slides coated with APTES were immersed in NaOH solution, enabling us to produce silver surfaces homogeneously covered with APTES. The surface properties of grafted APTES on sputtered silver surface as a occasion of time were studied using SPR analysis, AFM and contact angle measurement. The mechanical and chemical stability of samples was assayed by tape test and NaCl test. The answers show that hydrolysis and condensation of APTES are activated in alkaline solution and lead to formation of a protective APTES layer on the surface of silver. The morphology of APTES on silver surface is a function of coverage density that is altered by changing time. At short times (< 30 min, APTES molecules physically adsorb to the surface leads to weak protection. At higher condensation times, APTES molecules chemically bond to the surface and each other leads to better protection.

  10. WiSPR - A Graphical User Interface for Accessing a Sybase Database

    Science.gov (United States)

    Williamson, Ramon L., II

    WiSPR is a Tcl/Tk script in the X environment that builds a display of the fields of an arbitrary database table in an easy-to-read format on the fly. Each field of the database table has a text widget into which search strings for that field may be entered. When all desired search fields have been filled, an SQL query is constructed and values are returned into the text widgets one row at a time. Subsequent matches to the query are displayed until all matching rows have been retrieved. WiSPR recognizes all SQL wildcards when doing queries, and once values are returned, the results may be saved to a file or printed on a printer. In addition, if write access to the database in question is available, the user can add new rows to the database or update entries in a row already retrieved. On-line help is available at anytime using a menu-driven help system. Sybase database access is accomplished by means of the sybtcl library, written as an extension to Tcl/Tk by Tom Poindexter of Denver Colorado.

  11. Layer-by-layer assembled carbon nanotube-acetylcholinesterase/biopolymer renewable interfaces: SPR and electrochemical characterization.

    Science.gov (United States)

    Zhang, Yuanyuan; Arugula, Mary A; Kirsch, Jeffrey S; Yang, Xiaoyun; Olsen, Eric; Simonian, Aleksandr L

    2015-02-03

    Developing simple, reliable, and cost-effective methods of renewing an inhibited biocatalyst (e.g., enzymatic interfaces) on biosensors is needed to advance multiuse, reusable sensor applications. We report a method for the renewal of layer-by-layer (LbL) self-assembled inhibition-based enzymatic interfaces in multiwalled carbon nanotube (MWCNT) armored acetylcholinesterase (AChE) biosensors. The self-assembly process of MWCNT dispersed enzymes/biopolymers was investigated using surface plasmon resonance (SPR). The LbL fabrication consisted of alternating cushion layers of positively charged CNT-polyethylenimine (CNT-PEI) and negatively charged CNT-deoxyribonucleic acid (CNT-DNA) and a functional interface consisting of alternating layers of CNT-PEI and negatively charged CNT-acetylcholine esterase (CNT-AChE, pH 7.4). The observed SPR response signal increased while assembling the different layers, indicating the buildup of multiple layers on the Au surface. A partial desorption of the top enzymatic layer in the LbL structure was observed with a desorption strategy employing alkaline treatment. This indicates that the strong interaction of CNT-biopolymer conjugates with the Au surface was a result of both electrostatic interactions between biopolymers and the surface binding energy from CNTs: the closer the layers are to the Au surface, the stronger the interactions. In contrast, a similar LbL assembly of soluble enzyme/polyelectrolytes resulted in stronger desorption on the surface after the alkaline treatment; this led to the investigation of AChE layer removal, permanently inhibited after pesticide exposure on glassy carbon (GC) electrodes, while keeping the cushion layers intact. The desorption strategy permitted the SPR and electrochemical electrode surfaces to be regenerated multiple times by the subsequent self-assembly of fresh PEI/AChE layers. Flow-mode electrochemical amperometric analysis demonstrated good stability toward the determination of

  12. «[…] en enkel og saklig innføring i norsk språkhistorie» Språkhistorie som norskdidaktisk emne – et faghistorisk utsyn

    Directory of Open Access Journals (Sweden)

    Stian Hårstad

    2016-09-01

    Full Text Available Artikkelen tar for seg språkhistorie som norskdidaktisk emne i et faghistorisk perspektiv. På grunnlag av en lang rekke styringsdokumenter (læreplaner og studieplaner og fagdidaktisk og disiplinfaglig litteratur undersøkes hvordan språkets historiske dimensjoner har blitt tematisert som kunnskapsområde. Et hovedfunn i denne gjennomgangen er at det historiske (eller historiserende lengdesnittet har vært til stede gjennom hele 1900-tallet og fortsatt finnes i gjeldende læreplan – om enn med temmelig varierende vektlegging og detaljering. Læreplanene har i stor grad overlatt til praktikerne (lærerne og lærerutdannerne å avgrense hva de språkhistoriske emnene skal romme. Litteraturanalysen viser videre at den didaktiske refleksjonen på dette feltet bare unntaksvis har kommet på trykk, og denne begrensete språkhistorie-didaktiske debatten må forstås på bakgrunn av tilstanden i forsknings- og studiefaget.Nøkkelord: språkhistorie-didaktikk, språkdidaktikk, språkhistorie, norskdidaktikkAbstractThis article deals with language history as a didactic topic in the subject Norwegian as L1. On the basis of a wide range of documents (curricula and syllabi and didactic and disciplinary literature, the article seeks to examine how the historical dimensions of language have been treated as an area of knowledge. A main finding is that the historic (or historicizing perspective has been present through the entire 20th century and still can be found in the current curriculum – though the emphasis and itemization have varied significantly. The curricula have largely entrusted the practicians (the teachers and teacher educators with the powers to delineate the language history topics. The research review further shows that the didactic reflections within this field only sporadically have appeared in print, and this limited didactic discussion of language history must be understood on the background of the condition of the discipline

  13. High Sensitivity Transmission-Type SPR Sensor by Using Metallic-Dielectric Mixed Gratings

    Institute of Scientific and Technical Information of China (English)

    WU Bin; WANG Qing-Kang

    2008-01-01

    We theoretically investigate transmission-type SPR sensors with novel metallic-dielectric mixed gratings by rigorous coupled-wave analysis (RCWA),compared to the conventional dielectric gratings based structure.It is found that the transmittance efficiency and the full width at half-maximum (FWHM) of the transmission curve can be modulated by increasing or decreasing the metallic part.Therefore,appropriate proportion of metal part will induce enhancement factor of sensor merit.Furthermore,this novel structure will also bring enhancement of resonant angle shift,which can be explained by plasmonic interpretation based on a surface limited increase of interaction area and excitation of localized surface plasmons (LSPs).The proposed configuration has a wide range of potential applications not only as sensor but also other optical devices.

  14. Systém pro správu slovníkových dat

    OpenAIRE

    Šimek, Petr

    2014-01-01

    Bakalářská práce se zabývá elektronickými slovníky, které jsou uloženy ve formátu Lexical Markup Framework. Jejich analýzou správnosti převodu z jiných formátů, sjednocování použité terminologie a hromadnou změnou dat. Pro tyto účely byly vytvořeny skripty v jazyce Python3. The bachelor's thesis deals with electronic dictionaries that are stored in the format Lexical Markup Framework. It deals with their analysis of the correctness of the conversion to other formats, the unificat...

  15. Lyrikk og lindring: Om palliasjon og symboliske språk

    OpenAIRE

    Kleppe, Sandra Lee

    2013-01-01

    Raymond Carver regnes som en av USAs viktigste forfattere. Han døde av lungekreft bare 50 år gammel i 1988, i en tid da palliasjon var et nytt og lite synlig fagområde. Men Carvers lyrikk er siden blitt brukt i medisinundervisningen på en rekke kurs hvor studenter skal lære hvordan de kan håndtere døende pasienter. Flere av Carvers dikt setter søkelyset på situasjoner hvor kommunikasjonen mellom pleier og pasient er satt på prøve fordi de bruker svært forskjellige språk. Blant annet tar leger...

  16. Investigation of SnSPR1, a novel and abundant surface protein of Sarcocystis neurona merozoites.

    Science.gov (United States)

    Zhang, Deqing; Howe, Daniel K

    2008-04-15

    An expressed sequence tag (EST) sequencing project has produced over 15,000 partial cDNA sequences from the equine pathogen Sarcocystis neurona. While many of the sequences are clear homologues of previously characterized genes, a significant number of the S. neurona ESTs do not exhibit similarity to anything in the extensive sequence databases that have been generated. In an effort to characterize parasite proteins that are novel to S. neurona, a seemingly unique gene was selected for further investigation based on its abundant representation in the collection of ESTs and the predicted presence of a signal peptide and glycolipid anchor addition on the encoded protein. The gene was expressed in E. coli, and monospecific polyclonal antiserum against the recombinant protein was produced by immunization of a rabbit. Characterization of the native protein in S. neurona merozoites and schizonts revealed that it is a low molecular weight surface protein that is expressed throughout intracellular development of the parasite. The protein was designated Surface Protein 1 (SPR1) to reflect its display on the outer surface of merozoites and to distinguish it from the ubiquitous SAG/SRS surface antigens of the heteroxenous Coccidia. Interestingly, infection assays in the presence of the polyclonal antiserum suggested that SnSPR1 plays some role in attachment and/or invasion of host cells by S. neurona merozoites. The work described herein represents a general template for selecting and characterizing the various unidentified gene sequences that are plentiful in the EST databases for S. neurona and other apicomplexans. Furthermore, this study illustrates the value of investigating these novel sequences since it can offer new candidates for diagnostic or vaccine development while also providing greater insight into the biology of these parasites.

  17. Strategic Petroleum Reserve (SPR) additional geologic site characterization studies, Bryan Mound Salt Dome, Texas

    Energy Technology Data Exchange (ETDEWEB)

    Neal, J.T. [Sandia National Labs., Albuquerque, NM (United States); Magorian, T.R.; Ahmad, S. [Acres International Corp., Amherst, NY (United States)

    1994-11-01

    This report revises the original report that was published in 1980. Some of the topics covered in the earlier report were provisional and it is now practicable to reexamine them using new or revised geotechnical data and that obtained from SPR cavern operations, which involves 16 new caverns. Revised structure maps and sections show interpretative differences as compared with the 1980 report and more definition in the dome shape and caprock structural contours, especially a major southeast-northwest trending anomalous zone. The original interpretation was of westward tilt of the dome, this revision shows a tilt to the southeast, consistent with other gravity and seismic data. This interpretation refines the evaluation of additional cavern space, by adding more salt buffer and allowing several more caverns. Additional storage space is constrained on this nearly full dome because of low-lying peripheral wetlands, but 60 MMBBL or more of additional volume could be gained in six or more new caverns. Subsidence values at Bryan Mound are among the lowest in the SPR system, averaging about 11 mm/yr (0.4 in/yr), but measurement and interpretation issues persist, as observed values are about the same as survey measurement accuracy. Periodic flooding is a continuing threat because of the coastal proximity and because peripheral portions of the site are at elevations less than 15 ft. This threat may increase slightly as future subsidence lowers the surface, but the amount is apt to be small. Caprock integrity may be affected by structural features, especially the faulting associated with anomalous zones. Injection wells have not been used extensively at Bryan Mound, but could be a practicable solution to future brine disposal needs. Environmental issues center on the areas of low elevation that are below 15 feet above mean sea level: the coastal proximity and lowland environment combined with the potential for flooding create conditions that require continuing surveillance.

  18. Strategic Petroleum Reserve (SPR) additional geologic site characterization studies, Bryan Mound Salt Dome, Texas

    Energy Technology Data Exchange (ETDEWEB)

    Neal, J.T. [Sandia National Labs., Albuquerque, NM (United States); Magorian, T.R.; Ahmad, S. [Acres International Corp., Amherst, NY (United States)

    1994-11-01

    This report revises the original report that was published in 1980. Some of the topics covered in the earlier report were provisional and it is now practicable to reexamine them using new or revised geotechnical data and that obtained from SPR cavern operations, which involves 16 new caverns. Revised structure maps and sections show interpretative differences as compared with the 1980 report and more definition in the dome shape and caprock structural contours, especially a major southeast-northwest trending anomalous zone. The original interpretation was of westward tilt of the dome, this revision shows a tilt to the southeast, consistent with other gravity and seismic data. This interpretation refines the evaluation of additional cavern space, by adding more salt buffer and allowing several more caverns. Additional storage space is constrained on this nearly full dome because of low-lying peripheral wetlands, but 60 MMBBL or more of additional volume could be gained in six or more new caverns. Subsidence values at Bryan Mound are among the lowest in the SPR system, averaging about 11 mm/yr (0.4 in/yr), but measurement and interpretation issues persist, as observed values are about the same as survey measurement accuracy. Periodic flooding is a continuing threat because of the coastal proximity and because peripheral portions of the site are at elevations less than 15 ft. This threat may increase slightly as future subsidence lowers the surface, but the amount is apt to be small. Caprock integrity may be affected by structural features, especially the faulting associated with anomalous zones. Injection wells have not been used extensively at Bryan Mound, but could be a practicable solution to future brine disposal needs. Environmental issues center on the areas of low elevation that are below 15 feet above mean sea level: the coastal proximity and lowland environment combined with the potential for flooding create conditions that require continuing surveillance.

  19. Reflective-distributed SPR sensor based on twin-core fiber

    Science.gov (United States)

    Liu, Zhihai; Wei, Yong; Zhang, Yu; Zhu, Zongda; Zhao, Enming; Zhang, Yaxun; Yang, Jun; Liu, Chunyu; Yuan, Libo

    2016-05-01

    We propose and demonstrate a reflective-distributed surface plasmon resonance (SPR) sensor based on the twin-core fiber. Firstly, we study the effects of the fiber dual tapered (DT) probe grinding angle on the SPR dynamic range. The results show that for larger grinding angles, the resonance wavelength increases, resulting in a higher testing sensitivity. By using this method, we can make the sensor operate in an optimal waveband. Secondly, on the basis of the results above, we grind the DT probe to be an asymmetric wedge shape to configure two grinding angles (6° and 17°) to realize the distributed sensing. Results show that, with the refractive index detecting a range of 1.333-1.385, we can get two separated sensing zones, 591-715 nm and 729-966 nm, the testing sensitivity are 2385 nm/RIU and 4558 nm/RIU respectively. By using this method, we can detect multiple analytes in the same sensing area simultaneously, besides that we can also effectively compensate for the errors caused by background index interference, and the refractive index change resulting from the non-specific binding, or physical absorption and others. It worth to say that by using this method, we can adjust and control the resonance wavelength by changing the fiber grinding angle, while keeping the testing sensitivity is not reduced. For practical applications, this reflective distributed fiber-based sensor is much suitable for biochemical sensing, it has small size and can enter a small testing spaces (μm scale). The diameter of the twin-core fiber is the only 125 μm, which helps to be integrated into a micro-fluidic chip. In this paper, we integrate the fiber probe into an infusion needle to simulate blood vessels on-line monitoring.

  20. Measurement by SPR of very low dissociation rates: oxidation-mediated loss of biotin-streptavidin affinity.

    Science.gov (United States)

    Rebhan, Mario A E; Brunschweiger, Andreas; Hall, Jonathan

    2013-11-04

    Long-term relationship: biotin labels on RNAs, and possibly other biomacromolecules, are easily oxidized causing a dramatic loss of affinity for streptavidin and adversely affecting the measurement of high-affinity interactions. A new SPR method has been developed for measuring the very low rate-dissociation constants of biotin- and biotin oxide-conjugated RNAs with streptavidin.

  1. Benzimidazole Carbamate Residues in Milk: Detection by SPR Biosensor; using a Modified QuEChERS Method for Extraction

    Science.gov (United States)

    A surface plasmon resonance (SPR) biosensor screening assay was developed and validated to detect 11 benzimidazole carbamate (BZT) veterinary drug residues in milk. The polyclonal antibody used was raised in sheep against a methyl 5 (6)-[(carboxypentyl)-thio]-2-benzimidazole carbamate protein conjug...

  2. Cloning, expression and structural stability of a cold-adapted ß-Galactosidase from Rahnella sp.R3

    Science.gov (United States)

    A novel gene was isolated for the first time from a psychrophilic gram-negative bacterium Rahnella sp.R3. It encoded a cold-adapted ß-galactosidase (R-ß-Gal). Recombinant R-ß-Gal was expressed in Escherichia coli BL21 (DE3), purified, and characterized. R-ß-Gal belongs to the glycosyl hydrolase fami...

  3. Enhancement of chitosan-graphene oxide SPR sensor with a multi-metallic layers of Au-Ag-Au nanostructure for lead(II) ion detection

    Science.gov (United States)

    Kamaruddin, Nur Hasiba; Bakar, Ahmad Ashrif A.; Yaacob, Mohd Hanif; Mahdi, Mohd Adzir; Zan, Mohd Saiful Dzulkefly; Shaari, Sahbudin

    2016-01-01

    We demonstrate the enhancement of surface plasmon resonance (SPR) technique by implementing a multi-metallic layers of Au-Ag-Au nanostructure in the chitosan-graphene oxide (CS-GO) SPR sensor for lead(II) ion detection. The performance of the sensor is analyzed via SPR measurements, from which the sensitivity, signal-to-noise ratio and repeatability are determined. The nanostructure layers are characterized using field emission scanning electron microscopy (FESEM), atomic force microscopy (AFM), X-ray diffraction (XRD), Raman spectroscopy and X-ray photoelectron spectroscopy (XPS). We showed that the proposed structure has increased the shift in the SPR angle up to 3.5° within the range of 0.1-1 ppm due to the enhanced evanescent field at the sensing layer-analyte interface. This sensor also exhibits great repeatability which benefits from the stable multi-metallic nanostructure. The SNR value of 0.92 for 5 ppm lead(II) ion solution and reasonable linearity range up to that concentration shows that the tri-metallic CS-GO SPR sensor gives a good response towards the lead(II) ion solution. The CS-GO SPR sensor is also sensitive to at least a 10-5 change in the refractive index. The results prove that our proposed tri-metallic CS-GO SPR sensor demonstrates a strong performance and reliability for lead(II) ion detection in accordance with the standardized lead safety level for wastewater.

  4. Korpuslingvisten i klasserommet: lingvistiske profiler i fremtidens språkundervisning

    Directory of Open Access Journals (Sweden)

    Tore Nesset

    2014-09-01

    Full Text Available Den teknologiske utviklingen har gitt informasjonsalderens lingvister tilgang til mye større datamengder enn før. Mens datainnsamling bare for noen få år siden krevde mye tid og arbeid, er data i dag bare noen tastetrykk unna idet vi kan benytte oss av store elektroniske korpus. I denne artikkelen drøfter vi de konsekvensene den teknologiske utviklingen i korpuslingvistikken kan få for fremmedspråkundervisningen ved universiteter og høyskoler frem mot 2030. Vi foreslår at “lingvistiske profiler”, et sett av metoder utviklet i CLEAR-gruppen ved UiT Norges Arktiske Universitet, gjør det mulig å trekke veksler på innsikter fra korpuslingvistikken i fremmedspråkundervisningen. Tre typer lingvistiske profiler vil bli diskutert inngående: konstruksjonsprofiler, grammatiske profiler og radial kategori-profiler. Vi drøfter hvordan disse korpuslingvistiske metodene kan komme til nytte i klasserommet. Våre eksempler er fra russisk, men metodene er like anvendelige for andre språk, og artikkelen er ikke skrevet for eksperter på russisk.

  5. Theoretical modeling of a self-referenced dual mode SPR sensor utilizing indium tin oxide film

    Science.gov (United States)

    Srivastava, Sachin K.; Verma, Roli; Gupta, Banshi D.

    2016-06-01

    A prism based dual mode SPR sensor was theoretically modeled to work as a self-referenced sensor in spectral interrogation scheme. Self-referenced sensing was achieved by sandwiching an indium tin oxide thin film in between the prism base and the metal layer. The proposed sensor possesses two plasmon modes similar to long and short range SPRs (LR- and SR-SPRs) and we have analogically used LRSPR and SRSPR for them. However, these modes do not possess usual long range character due to the losses introduced by the imaginary part of indium tin oxide (ITO) dielectric function. One of the two plasmon modes responds to change in analyte refractive index while the other remains fixed. The influence of various design parameters on the performance of the sensor was evaluated. The performance of the proposed sensor was compared, via control simulations, with established dual mode geometries utilizing silicon dioxide (SiO2), Teflon AF-1600 and Cytop. The design parameters of the established geometries were optimized to obtain self-referenced sensing operation. Trade-offs between the resonance spectral width, minimum reflectivity, shift in resonance wavelength and angle of incidence were examined for optimal design. The present study will be useful in the fabrication of self-referenced sensors where the ambient conditions are not quite stable.

  6. Správa vozidel za pomoci mobilního telefonu s GPS

    OpenAIRE

    Pompe, Patrik

    2012-01-01

    Tato práce se zabývá získáváním informací z chytrého mobilního telefonu pro využití v aplikaci typu kniha jízd. Nejprve specifikuje a analyzuje požadavky na takovou aplikaci, poté sezabývá možnostmi její realizace. Popisuje implementaci zvoleného řešení, které se skládá ze tří částí - aplikace pro telefon na platformě Android, která sbírá potřebné informace o trasách vozidel, webového portálu, který poskytuje rozhraní pro zobrazení a správu získaných dat a webových služeb pro zajištění komuni...

  7. Development of SPR Immunosensor by Indirect Competitive Method for Rapid and Highly Sensitive Salivary Cortisol Detection

    Directory of Open Access Journals (Sweden)

    Yusuke eTahara

    2014-05-01

    Full Text Available The monitoring of salivary cortisol as a key biomarker of an individual’s stress response has been increasingly focused on. This paper describes the development of a novel cortisol biosensor based on an indirect competitive method using a surface plasmon resonance (SPR immunosensor. The surface of a Au chip was modified with PEG6-COOH aromatic dialkanethiol self-assembled monolayers (SAMs and hydrocortisone 3-(O-carboxymethyl oxime (hydrocortisone 3-CMO as a cortisol analogue. A detection limit of 38 ppt range with a measurement range of 10 ppt to 100 ppb was accomplished without the incubation of a mixing solution consisting of standard cortisol and an anti-cortisol antibody, and the response time was 5 min from the sample injection. We experimentally compared our biosensor with a commercialized salivary cortisol enzyme-linked immunosorbent assay (ELISA kit using human saliva samples. It was found that the results obtained by the cortisol biosensor had a high correlation with those obtained by ELISA assay (R = 0.96. Our findings indicate the potential utility of the cortisol biosensor for on-site diagnosis and bedside point-of-care testing (POCT from bedside testing.

  8. Fast and sensitive detection of ochratoxin A in red wine by nanoparticle-enhanced SPR.

    Science.gov (United States)

    Karczmarczyk, Aleksandra; Reiner-Rozman, Ciril; Hageneder, Simone; Dubiak-Szepietowska, Monika; Dostálek, Jakub; Feller, Karl-Heinz

    2016-09-21

    Herein, we present a fast and sensitive biosensor for detection of Ochratoxin A (OTA) in a red wine that utilizes gold nanoparticle-enhanced surface plasmon resonance (SPR). By combining an indirect competitive inhibition immunoassay and signal enhancement by secondary antibodies conjugated with gold nanoparticles (AuNPs), highly sensitive detection of low molecular weight compounds (such as OTA) was achieved. The reported biosensor allowed for OTA detection at concentrations as low as 0.75 ng mL(-1) and its limit of detection was improved by more than one order of magnitude to 0.068 ng mL(-1) by applying AuNPs as a signal enhancer. The study investigates the interplay of size of AuNPs and affinity of recognition elements affecting the efficiency of the signal amplification strategy based on AuNP. Furthermore, we observed that the presence of polyphenolic compounds in wine samples strongly interferes with the affinity binding on the surface. To overcome this limitation, a simple pre-treatment of the wine sample with the binding agent poly(vinylpyrrolidone) (PVP) was successfully applied.

  9. SPR and electrochemical analyses of interactions between CYP3A4 or 3A5 and cytochrome b5

    Science.gov (United States)

    Gnedenko, O. V.; Yablokov, E. O.; Usanov, S. A.; Mukha, D. V.; Sergeev, G. V.; Bulko, T. V.; Kuzikov, A. V.; Moskaleva, N. E.; Shumyantseva, V. V.; Ivanov, A. S.; Archakov, A. I.

    2014-02-01

    The combination of SPR biosensor with electrochemical analysis was used for the study of protein-protein interaction between cytochromes CYP3A4 or 3А5 and cytochromes b5: the microsomal, mitochondrial forms of this protein, and 2 ≪chimeric≫ proteins. Kinetic constants of CYP3A4 and CYP3А5 complex formation with cytochromes b5 were determined by the SPR biosensor. Essential distinction between CYP3A4 and CYP3A5 was observed upon their interactions with mitochondrial cytochrome b5. The electrochemical analysis of CYP3A4, CYP3A5, and cytochromes b5 immobilized on screen printed graphite electrodes modified with membranous matrix revealed that these proteins have very close reduction potentials -0.435 to -0.350 V (vs. Ag/AgCl).

  10. SPR Characteristics Curve and Distribution of Residual Stress in Self-Piercing Riveted Joints of Steel Sheets

    Directory of Open Access Journals (Sweden)

    Rezwanul Haque

    2017-01-01

    Full Text Available Neutron diffraction was used to describe the residual stress distributions in self-piercing riveted (SPR joints. The sheet material displayed a compressive residual stress near the joint, and the stress gradually became tensile in the sheet material far away from the joint. The stress in the rivet leg was lower in the thick joint of the softer steel sheet than in the thin joint of the harder steel sheet. This lower magnitude was attributed to the lower force gradient during the rivet flaring stage of the SPR process curve. This study shows how the residual stress results may be related to the physical occurrences that happened during joining, using the characteristics curve. The study also shows that neutron diffraction technique enabled a crack in the rivet tip to be detected which was not apparent from a cross-section.

  11. Synergy between cellulolytic enzymes during the biodegradation of cellulose microfibrils measured using angle-scanning surface plasmon resonance (SPR) imaging

    Science.gov (United States)

    Raegen, Adam; Dion, Alexander; Reiter, Kyle; Clarke, Anthony; Lipkowski, Jacek; Dutcher, John

    2014-03-01

    The use of cellulosic ethanol, a promising emerging energy source, is limited by the energy intensive and costly step of first converting the cellulose fibers into their constituent glucose monomers. Industrial processes mimic those that occur in nature, using mixtures or ``cocktails'' of different classes of cellulolytic enzymes derived from fungi. Despite several decades of investigation, the molecular mechanisms for enzyme synergy remain poorly understood. To gain additional insight, we have used a custom angle-scanning surface plasmon resonance (SPR) imaging apparatus to obtain a sensitive measure of enzymatic degradation. By implementing a novel SPR data analysis procedure, we have been able to track the thickness and roughness of laterally heterogeneous cellulose microfibril-coated substrates as enzymatic degradation proceeds. This has allowed us to measure the synergistic actions of the different enzymes, providing data that are directly relevant to the cellulosic ethanol industry.

  12. Detection of trinitrotoluene (TNT) extracted from soil using a surface plasmon resonance (SPR)-based sensor platform

    Science.gov (United States)

    Strong, Anita A.; Stimpson, Donald I.; Bartholomew, Dwight U.; Jenkins, Thomas F.; Elkind, Jerome L.

    1999-08-01

    An antibody-based competition assay has been developed using a surface plasmon resonance (SPR) sensor platform for the detection of trinitrotoluene (TNT) in soil extract solutions. The objective of this work is to develop a sensor-based assay technology to use in the field for real- time detection of land mines. This immunoassay combines very simple bio-film attachment procedures and a low-cost SPR sensor design to detect TNT in soil extracts. The active bio-surface is a coating of bovine serum albumin that has been decorated with trinitrobenzene groups. A blind study on extracts from a large soil matrix was recently performed and result from this study will be presented. Potential interferant studied included 2,4-dinitrophenol, 2,4- dinitrotoluene, ammonium nitrate, and 2,4- dichlorophenoxyacetic acid. Cross-reactivity with dinitrotoluene will be discussed. Also, plans to reach sensitivity levels of 1ppb TNT in soil will be described.

  13. Herstellung und Charakterisierung von Kombinationen aus Mannit und Sorbit durch Sprühtrocknung und Co-Granulation

    OpenAIRE

    Bauer, Heike

    2000-01-01

    Ziel der Arbeit ist es, eine Polyolkombination aus Mannit und Sorbit herzustellen und die Parameter für den Granulationsprozess festzulegen, unter denen eine nadelförmige Struktur auf der Partikeloberfläche entsteht. Eine gegenseitige Beeinflussung der beiden Komponenten sowie auftretende Polymorphie wird an physikalischen Mischungen und co-sprühgetrockneten Produkten untersucht. Es entstehen weder Co-Kristalle noch ein Eutektikum. Die Polyole kristallisieren nebeneinander aus. Sorbit fällt h...

  14. Fiber optic-SPR platform for fast and sensitive infliximab detection in serum of inflammatory bowel disease patients.

    Science.gov (United States)

    Lu, Jiadi; Van Stappen, Thomas; Spasic, Dragana; Delport, Filip; Vermeire, Séverine; Gils, Ann; Lammertyn, Jeroen

    2016-05-15

    Infliximab (IFX) is a therapeutic monoclonal antibody used for treating patients with inflammatory bowel disease (IBD). In order to improve therapeutic outcomes it is recommended to monitor IFX trough concentrations. Although ELISA is currently widely used for this purpose, this method is not suitable for single patient testing. In this paper we describe the development of a fast bioassay for determining IFX concentration in serum using an in-house developed fiber-optic surface plasmon resonance (FO-SPR) biosensor. Studies were first conducted to optimize covalent immobilization of the IFX-specific antibody on the sensor surface as well as to select an optimal blocking buffer for restraining the non-specific binding. In order to reach clinically relevant sensitivity for detecting IFX in patients' serum, the SPR signal was amplified by employing gold nanoparticles functionalized with another set of IFX specific antibodies. Using the optimized sandwich bioassay, calibration curves were made with series of IFX concentrations spiked in buffer and 100-fold diluted serum, reaching the limit of detection of 0.3 and 2.2ng/ml, respectively. The established bioassay was finally validated using five IFX treated IBD patients samples. Results from the FO-SPR platform were compared with an in-house developed, clinically validated ELISA resulting in excellent Pearson and intraclass correlation coefficient of 0.998 and 0.983, respectively. Furthermore, the assay time of the FO-SPR platform was significantly reduced compared to ELISA, demonstrating the potential of this platform to be used as a point-of-care diagnostic tool for improving therapeutic outcomes of IBD patients.

  15. Immobilization of flavan-3-ols onto sensor chips to study their interactions with proteins and pectins by SPR

    Energy Technology Data Exchange (ETDEWEB)

    Watrelot, Aude A., E-mail: aude.watrelot@avignon.inra.fr [INRA, UMR408 Sécurité et Qualité des Produits d’Origine Végétale, Domaine St Paul, Site Agroparc, 84914 Avignon (France); Université d’Avignon, UMR408 Sécurité et Qualité des Produits d' Origine Végétale, F-84000 Avignon (France); Tran, Dong Tien [Université de Bordeaux, Institut des Sciences Moléculaires (UMR-CNRS 5255), 351 cours de la Libération, 33405 Talence (France); Institut Européen de Chimie et Biologie (IECB), 2 rue Robert Escarpit, 33607 Pessac (France); Buffeteau, Thierry [Université de Bordeaux, Institut des Sciences Moléculaires (UMR-CNRS 5255), 351 cours de la Libération, 33405 Talence (France); Deffieux, Denis [Université de Bordeaux, Institut des Sciences Moléculaires (UMR-CNRS 5255), 351 cours de la Libération, 33405 Talence (France); Institut Européen de Chimie et Biologie (IECB), 2 rue Robert Escarpit, 33607 Pessac (France); and others

    2016-05-15

    Highlights: • Flavanol-macromolecule interactions were determined using SPR. • Flavanols were chemically modified with a linker bearing a thiol group. • Flavanols were immobilized onto a carboxymethyl dextran surface. • Citrus pectin interacted more with flavanols than apple pectin. • Epicatechin interacted more with BSA than flavanol oligomer. - Abstract: Interactions between plant polyphenols and biomacromolecules such as proteins and pectins have been studied by several methods in solution (e.g. isothermal titration calorimetry, dynamic light scattering, nuclear magnetic resonance and spectrophotometry). Herein, these interactions were investigated in real time by Surface Plasmon Resonance (SPR) analysis after immobilization of flavan-3-ols onto a sensor chip surface. (−)-epicatechin, (+)-catechin and flavan-3-ol oligomers with an average degree of polymerization of 2 and 8 were chemically modified using N-(2-(tritylthio)ethyl)propiolamide in order to introduce a spacer unit onto the catecholic B ring. Modified flavan-3-ols were then immobilized onto a carboxymethylated dextran surface (CM5). Immobilization was validated and further verified by evaluating flavan-3-ol interaction with bovine serum albumin (BSA), poly-L-proline or commercial pectins. BSA was found to have a stronger association with monomeric flavan-3-ols than oligomers. SPR analysis of selected flavan-3-ols immobilized onto CM5 sensor chips showed a stronger association for citrus pectins than apple pectins, regardless of flavan-3-ol degree of polymerization.

  16. Kinetic analysis of IgM monoclonal antibodies for determination of dengue sample concentration using SPR technique.

    Science.gov (United States)

    Jahanshahi, Peyman; Wei, Qin; Jie, Zhang; Ghomeishi, Mostafa; Sekaran, Shamala Devi; Mahamd Adikan, Faisal Rafiq

    2016-08-17

    Surface plasmon resonance (SPR) sensing is recently emerging as a valuable technique for measuring the binding constants, association and dissociation rate constants, and stoichimetry for a binding interaction kinetics in a number of emerging biological areas. This technique can be applied to the study of immune system diseases in order to contribute to improved understanding and evaluation of binding parameters for a variety of interactions between antigens and antibodies biochemically and clinically. Since the binding constants determination of an anti-protein dengue antibody (Ab) to a protein dengue antigen (Ag) is mostly complicated, the SPR technique aids a determination of binding parameters directly for a variety of particular dengue Ag_Ab interactions in the real-time. The study highlights the doctrine of real-time dengue Ag_Ab interaction kinetics as well as to determine the binding parameters that is performed with SPR technique. In addition, this article presents a precise prediction as a reference curve for determination of dengue sample concentration.

  17. Study of Immobilization Procedure on Silver Nanolayers and Detection of Estrone with Diverged Beam Surface Plasmon Resonance (SPR Imaging

    Directory of Open Access Journals (Sweden)

    Ibrahim Abdulhalim

    2013-03-01

    Full Text Available An immobilization protocol was developed to attach receptors on smooth silver thin films. Dense and packed 11-mercaptoundecanoic acid (11-MUA was used to avoid uncontrolled sulfidization and harmful oxidation of silver nanolayers. N,N'-dicyclohexylcarbodiimide (DCC and N-hydroxysuccinimide (NHS were added to make the silver surfaces reactive. A comparative study was carried out with different immersion times of silver samples in 11-MUA solutions with different concentrations to find the optimum conditions for immobilization. The signals, during each step of the protocol, were analyzed with a refractometer based on the surface plasmon resonance (SPR effect and luminescence techniques. Molecular interactions at the surfaces between the probe and target at the surface nanolayer shift the SPR signal, thus indicating the presence of the substance. To demonstrate specific biosensing, rabbit anti-estrone polyclonal immunoglobulin G (IgG antibody was immobilized through a linker on 47 nm silver layer deposited on SF11 glass. At the final stage, the representative endocrine disruptor—estrone—was attached and detected in deionized water with a diverging beam SPR imaging sensor.

  18. Layer-by-layer assembled multilayer of graphene/Prussian blue toward simultaneous electrochemical and SPR detection of H2O2

    DEFF Research Database (Denmark)

    Mao, Yan; Bao, Yu; Wang, Wei

    2011-01-01

    A new type of chemically converted graphene sheets, cationic polyelectrolyte-functionalized ionic liquid decorated graphene sheets (PFIL–GS) composite, was synthesized and characterized by Ultraviolet–visible (UV–vis) absorption, Fourier transform infrared, and Raman spectroscopy. It was found...... layer-by-layer (LBL) assembly. The assembly process was confirmed by UV–vis spectroscopy and surface plasmon resonance (SPR) spectroscopy, which showed linear responses to the numbers of the deposited PFIL–GS/PB bilayers. Moreover, the as-prepared composite films were used to detect hydrogen peroxide (H......2O2) by electrochemical surface plasmon resonance (EC-SPR) spectroscopy. This real time EC-SPR technique can provide simultaneous monitoring of both optical SPR signal and electrochemical current responses upon injecting H2O2 into the reaction cell. The experimental results revealed that both...

  19. Enhancement of chitosan-graphene oxide SPR sensor with a multi-metallic layers of Au–Ag–Au nanostructure for lead(II) ion detection

    Energy Technology Data Exchange (ETDEWEB)

    Kamaruddin, Nur Hasiba [Department of Electric, Electronic and Systems Engineering, Faculty of Engineering and Built Environment, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor (Malaysia); Bakar, Ahmad Ashrif A., E-mail: ashrif@ukm.edu.my [Department of Electric, Electronic and Systems Engineering, Faculty of Engineering and Built Environment, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor (Malaysia); Yaacob, Mohd Hanif; Mahdi, Mohd Adzir [Wireless and Photonic Network Research Centre, Faculty of Engineering, Universiti Putra Malaysia, 43400 Serdang, Selangor (Malaysia); Zan, Mohd Saiful Dzulkefly [Department of Electric, Electronic and Systems Engineering, Faculty of Engineering and Built Environment, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor (Malaysia); Shaari, Sahbudin [Institute of Microengineering and Nanoelectronics, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor (Malaysia)

    2016-01-15

    Highlights: • Tri-metallic Au–Ag–Au CS-GO SPR sensor was fabricated for the first time. • The tri-metallic nanostructure provided an enhanced evanescent field. • Successful functionalization of the CS-GO sensing layer. • Superior performance for lead(II) ion detection. - Abstract: We demonstrate the enhancement of surface plasmon resonance (SPR) technique by implementing a multi-metallic layers of Au–Ag–Au nanostructure in the chitosan-graphene oxide (CS-GO) SPR sensor for lead(II) ion detection. The performance of the sensor is analyzed via SPR measurements, from which the sensitivity, signal-to-noise ratio and repeatability are determined. The nanostructure layers are characterized using field emission scanning electron microscopy (FESEM), atomic force microscopy (AFM), X-ray diffraction (XRD), Raman spectroscopy and X-ray photoelectron spectroscopy (XPS). We showed that the proposed structure has increased the shift in the SPR angle up to 3.5° within the range of 0.1–1 ppm due to the enhanced evanescent field at the sensing layer-analyte interface. This sensor also exhibits great repeatability which benefits from the stable multi-metallic nanostructure. The SNR value of 0.92 for 5 ppm lead(II) ion solution and reasonable linearity range up to that concentration shows that the tri-metallic CS-GO SPR sensor gives a good response towards the lead(II) ion solution. The CS-GO SPR sensor is also sensitive to at least a 10{sup −5} change in the refractive index. The results prove that our proposed tri-metallic CS-GO SPR sensor demonstrates a strong performance and reliability for lead(II) ion detection in accordance with the standardized lead safety level for wastewater.

  20. Aplicações de QCM, EIS e SPR na investigação de superfícies e interfaces para o desenvolvimento de (biosensores

    Directory of Open Access Journals (Sweden)

    Damos Flavio Santos

    2004-01-01

    Full Text Available The use of the quartz crystal microbalance process, electrochemical impedance spectroscopy and surface plasmon resonance for characterizing thin films and monitoring interfaces is presented. The theorical aspects of QCM, EIS and SPR are introduced and the main application areas are outlined. Future prospects of the combined applications of QCM, EIS and SPR methods in the studies of interfacial processes at surfaces are also discussed.

  1. Strategic Petroleum Reserve (SPR) additional geologic site characterization studies, Bayou Choctaw salt dome, Louisiana

    Energy Technology Data Exchange (ETDEWEB)

    Neal, J.T. [Sandia National Labs., Albuquerque, NM (United States); Magorian, T.R. [Magorian (Thomas R.), Amherst, NY (United States); Byrne, K.O.; Denzler, S. [Acres International Corp., Amherst, NY (United States)

    1993-09-01

    This report revises and updates the geologic site characterization report that was published in 1980. Revised structure maps and sections show interpretative differences in the dome shape and caprock structural contours, especially a major east-west trending shear zone, not mapped in the 1980 report. Excessive gas influx in Caverns 18 and 20 may be associated with this shear zone. Subsidence values at Bayou Choctaw are among the lowest in the SPR system, averaging only about 10 mm/yr but measurement and interpretation issues persist, as observed values often approximate measurement accuracy. Periodic, temporary flooding is a continuing concern because of the low site elevation (less than 10 ft), and this may intensify as future subsidence lowers the surface even further. Cavern 4 was re-sonared in 1992 and the profiles suggest that significant change has not occurred since 1980, thereby reducing the uncertainty of possible overburden collapse -- as occurred at Cavern 7 in 1954. Other potential integrity issues persist, such as the proximity of Cavern 20 to the dome edge, and the narrow web separating Caverns 15 and 17. Injection wells have been used for the disposal of brine but have been only marginally effective thus far; recompletions into more permeable lower Pleistocene gravels may be a practical way of increasing injection capacity and brinefield efficiency. Cavern storage space is limited on this already crowded dome, but 15 MMBBL could be gained by enlarging Cavern 19 and by constructing a new cavern beneath and slightly north of abandoned Cavern 13. Environmental issues center on the low site elevation: the backswamp environment combined with the potential for periodic flooding create conditions that will require continuing surveillance.

  2. Strategic Petroleum Reserve (SPR) additional geologic site characterization studies, Bayou Choctaw salt dome, Louisiana

    Energy Technology Data Exchange (ETDEWEB)

    Neal, J.T. [Sandia National Labs., Albuquerque, NM (United States); Magorian, T.R. [Magorian (Thomas R.), Amherst, NY (United States); Byrne, K.O.; Denzler, S. [Acres International Corp., Amherst, NY (United States)

    1993-09-01

    This report revises and updates the geologic site characterization report that was published in 1980. Revised structure maps and sections show interpretative differences in the dome shape and caprock structural contours, especially a major east-west trending shear zone, not mapped in the 1980 report. Excessive gas influx in Caverns 18 and 20 may be associated with this shear zone. Subsidence values at Bayou Choctaw are among the lowest in the SPR system, averaging only about 10 mm/yr but measurement and interpretation issues persist, as observed values often approximate measurement accuracy. Periodic, temporary flooding is a continuing concern because of the low site elevation (less than 10 ft), and this may intensify as future subsidence lowers the surface even further. Cavern 4 was re-sonared in 1992 and the profiles suggest that significant change has not occurred since 1980, thereby reducing the uncertainty of possible overburden collapse -- as occurred at Cavern 7 in 1954. Other potential integrity issues persist, such as the proximity of Cavern 20 to the dome edge, and the narrow web separating Caverns 15 and 17. Injection wells have been used for the disposal of brine but have been only marginally effective thus far; recompletions into more permeable lower Pleistocene gravels may be a practical way of increasing injection capacity and brinefield efficiency. Cavern storage space is limited on this already crowded dome, but 15 MMBBL could be gained by enlarging Cavern 19 and by constructing a new cavern beneath and slightly north of abandoned Cavern 13. Environmental issues center on the low site elevation: the backswamp environment combined with the potential for periodic flooding create conditions that will require continuing surveillance.

  3. Stepping Library-Based Post-SELEX Strategy Approaching to the Minimized Aptamer in SPR.

    Science.gov (United States)

    He, Xiaoqin; Guo, Lei; He, Junlin; Xu, Hua; Xie, Jianwei

    2017-06-20

    When evolved from SELEX (systematic evolution of ligands by exponential enrichment), aptamers are generally about 70-130 nucleotides in length and needed to be effectively truncated for further diagnosis or therapeutic uses. Post-SELEX optimization is then aroused to simplify the aptamer sequence and improve the affinity property. In this work, we report a new post-SELEX strategy based on a stepping library for the first time. With a hypothesis that one nucleobase can influence the whole binding affinity through its adjacent base stacking and potential steric hydrogen bonding interaction, we designed a stepping library composed of all probable nucleotide truncation directions. We employed an aptamer 807-39nt toward EPO-α as a model, and surface plasmon resonance (SPR) as an efficient screening and evaluation method to optimize all label-free sequences in the library. We have successfully picked out In27 as the minimized aptamer from a mini library of only 35 sequences. Aptamer In27 has a sole loop, without the original stem portion of the initial aptamer, but retains the whole binding affinity. We have also defined the key nucleotide contribution by site mutagenesis with natural bases, and finally produced a degenerated sequence with higher or the same good affinities. Furthermore, we explored different binding behaviors between aptamer In27 and other recognition molecule such as agglutinin, monoclonal antibody, or receptor by competition or binding assays. Our work provides a new and efficient post-SELEX optimization strategy, as well as a minimized aptamer In27 with an explicit degenerated sequence and a defined binding behavior. That would enhance their great potential in future diagnosis and therapy.

  4. Invention of stimulus-responsive peptide-bond-cleaving residue (Spr) and its application to chemical biology tools.

    Science.gov (United States)

    Shigenaga, Akira; Yamamoto, Jun; Kohiki, Taiki; Inokuma, Tsubasa; Otaka, Akira

    2017-07-01

    Elucidation of biological functions of peptides and proteins is essential for understanding peptide/protein-related biological events and developing drugs. Caged peptides and proteins that release a parent active peptide/protein by photo-irradiation have successfully been employed to elucidate the functions. Whereas the usual caged peptide/protein enables conversion of an inactive form to an active form (OFF-to-ON conversion) by photo-induced deprotection, photo-triggered main chain cleavage is reported to be applicable to ON-to-OFF conversion. These peptides and proteins are photo-responsive; however, if peptides and proteins could respond to other stimuli such as disease-related environment or enzymes, their range of application should be widened. To convert the photo-responsive peptide/protein into other stimulus-responsive peptide/protein, quite laborious de novo design and synthesis of the stimulus-responsive unit are required. In this context, we designed a stimulus-responsive peptide-bond-cleaving residue (Spr) in which the stimuli available for the main chain cleavage vary according to the choice of protecting groups on the residue. In this review, design and synthesis of Spr are introduced, and challenges to apply Spr to other fields to enable, for example, functional control, localization control, delivery of cargos, labeling of a protein of interest in living cells, and identification of target proteins of bioactive ligands are discussed. Copyright © 2017 European Peptide Society and John Wiley & Sons, Ltd. Copyright © 2017 European Peptide Society and John Wiley & Sons, Ltd.

  5. DNA-Aptamer optical biosensors based on a LPG-SPR optical fiber platform for point-of-care diagnostic

    Science.gov (United States)

    Coelho, L.; Queirós, R. B.; Santos, J. L.; Martins, M. Cristina L.; Viegas, D.; Jorge, P. A. S.

    2014-03-01

    Surface Plasmon Resonance (SPR) is the base for some of the most sensitive label free optical fiber biosensors. However, most solutions presented to date require the use of fragile fiber optic structure such as adiabatic tapers or side polished fibers. On the other hand, long-period fiber gratings (LPG) present themselves as an interesting solution to attain an evanescent wave refractive index sensor platform while preserving the optical fiber integrity. The combination of these two approaches constitute a powerful platform that can potentially reach the highest sensitivities as it was recently demonstrated by detailed theoretical study [1, 2]. In this work, a LPG-SPR platform is explored in different configurations (metal coating between two LPG - symmetric and asymmetric) operating in the telecom band (around 1550 nm). For this purpose LPGs with period of 396 μm are combined with tailor made metallic thin films. In particular, the sensing regions were coated with 2 nm of chromium to improve the adhesion to the fiber and 16 nm of gold followed by a 100 nm thick layer of TiO2 dielectric material strategically chosen to attain plasmon resonance in the desired wavelength range. The obtained refractometric platforms were then validated as a biosensor. For this purpose the detection of thrombin using an aptamer based probe was used as a model system for protein detection. The surface of the sensing fibers were cleaned with isopropanol and dried with N2 and then the aminated thrombin aptamer (5'-[NH2]- GGTTGGTGTGGTTGG-3') was immobilized by physisorption using Poly-L-Lysine (PLL) as cationic polymer. Preliminary results indicate the viability of the LPFG-SPR-APTAMER as a flexible platforms point of care diagnostic biosensors.

  6. Synthesis of surface plasmon resonance (SPR) triggered Ag/TiO{sub 2} photocatalyst for degradation of endocrine disturbing compounds

    Energy Technology Data Exchange (ETDEWEB)

    Leong, Kah Hon; Gan, Bee Ling; Ibrahim, Shaliza [Environmental Engineering Laboratory, Department of Civil Engineering, Faculty of Engineering, University of Malaya, 50603 Kuala Lumpur (Malaysia); Saravanan, Pichiah, E-mail: saravananpichiah@um.edu.my [Environmental Engineering Laboratory, Department of Civil Engineering, Faculty of Engineering, University of Malaya, 50603 Kuala Lumpur (Malaysia); Nanotechnology and Catalysis Research Center (NANOCAT), University of Malaya, 50603 Kuala Lumpur (Malaysia)

    2014-11-15

    Graphical abstract: - Highlights: • Ag/TiO{sub 2} was synthesized with aid of natural photon stimulated photoreduction. • Deposited Ag prompted well the LSPRs, Schottky barrier for visible light utilization. • Photocatalytic activity was evaluated by degrading EDCs under visible light. • 3.0 wt% Ag/TiO{sub 2} resulted with good photocatalytic efficiency over others. - Abstract: Surface deposition of silver nanoparticles (Ag NPs) onto the 100% anatase titania (Ag/TiO{sub 2}) for evolution of surface plasmon resonance (SPR) was achieved sustainably with the assistance of solar energy. The preparation resulted in Ag/TiO{sub 2} photocatalyst with varied Ag depositions (0.5 wt%, 1.0 wt%, 3.0 wt% and 5.0 wt%). All obtained photocatalysts were characterized for the evolution of SPR via crystalline phase analysis, morphology, lattice fringes, surface area and pore size characteristics, chemical composition with chemical and electronic state, Raman scattering, optical and photoluminescence properties. The deposition of synthesized Ag NPs exhibited high uniformity and homogeneity and laid pathway for effective utilization of the visible region of electromagnetic spectrum through SPR. The depositions also lead for suppressing recombination rates of electron–hole. The photocatalytic evaluation was carried out by adopting two different class of endocrine disturbing compound (EDC) i.e., amoxicillin (pharmaceutical) and 2,4-dichlorophenol (pesticide) excited with artificial visible light source. Ag/TiO{sub 2} with Ag > 0.5 wt% exhibited significant degradation efficiency for both amoxicillin and 2,4-dichlorophenol. Thus synthesized Ag/TiO{sub 2} revealed the implication of plasmonics on TiO{sub 2} for the enhanced visible light photocatalytic activity.

  7. Experiences from Nordic research collaboration in linguistics

    Directory of Open Access Journals (Sweden)

    Helge Sandøy

    2007-04-01

    Full Text Available The project “Modern loanwords in the languages of the Nordic countries (MIN – Moderne importord i språka i Norden” was the first large-scale collaborative project between linguists in the Nordic countries. This article presents both the aim of the project and some experiences from the work with respect to project design, financing and networking.

  8. Label-free SPR detection of gluten peptides in urine for non-invasive celiac disease follow-up.

    Science.gov (United States)

    Soler, Maria; Estevez, M-Carmen; Moreno, Maria de Lourdes; Cebolla, Angel; Lechuga, Laura M

    2016-05-15

    Motivated by the necessity of new and efficient methods for dietary gluten control of celiac patients, we have developed a simple and highly sensitive SPR biosensor for the detection of gluten peptides in urine. The sensing methodology enables rapid and label-free quantification of the gluten immunogenic peptides (GIP) by using G12 mAb. The overall performance of the biosensor has been in-depth optimized and evaluated in terms of sensitivity, selectivity and reproducibility, reaching a limit of detection of 0.33 ng mL(-1). Besides, the robustness and stability of the methodology permit the continuous use of the biosensor for more than 100 cycles with excellent repeatability. Special efforts have been focused on preventing and minimizing possible interferences coming from urine matrix enabling a direct analysis in this fluid without requiring extraction or purification procedures. Our SPR biosensor has proven to detect and identify gluten consumption by evaluating urine samples from healthy and celiac individuals with different dietary gluten conditions. This novel biosensor methodology represents a novel approach to quantify the digested gluten peptides in human urine with outstanding sensitivity in a rapid and non-invasive manner. Our technique should be considered as a promising opportunity to develop Point-of-Care (POC) devices for an efficient, simple and accurate gluten free diet (GFD) monitoring as well as therapy follow-up of celiac disease patients.

  9. Interaction between a cationic porphyrin and ctDNA investigated by SPR, CV and UV-vis spectroscopy.

    Science.gov (United States)

    Xu, Zi-Qiang; Zhou, Bo; Jiang, Feng-Lei; Dai, Jie; Liu, Yi

    2013-10-01

    The interaction between ctDNA and a cationic porphyrin was studied in this work. The binding process was monitored by surface plasmon resonance (SPR) spectroscopy in detail. The association, dissociation rate constants and the binding constants calculated by global analysis were 2.4×10(2)±26.4M(-1)s(-1), 0.011±0.0000056s(-1) and 2.18×10(4)M(-1), respectively. And the results were confirmed by cyclic voltammetry and UV-vis absorption spectroscopy. The binding constants obtained from cyclic voltammetry and UV-vis absorption spectroscopy were 8.28×10(4)M(-1) and 6.73×10(4)M(-1) at 298K, respectively. The covalent immobilization methodology of ctDNA onto gold surface modified with three different compounds was also investigated by SPR. These compounds all contain sulfydryl but with different terminated functional groups. The results indicated that the 11-MUA (HS(CH2)10COOH)-modified gold film is more suitable for studying the DNA-drug interaction.

  10. Antibody-Hapten Recognition at the Surface of Functionalized Liposomes Studied by SPR: Steric Hindrance of Pegylated Phospholipids in Stealth Liposomes Prepared for Targeted Radionuclide Delivery

    Directory of Open Access Journals (Sweden)

    Eliot. P. Botosoa

    2011-01-01

    Full Text Available Targeted PEGylated liposomes could increase the amount of drugs or radionuclides delivered to tumor cells. They show favorable stability and pharmacokinetics, but steric hindrance of the PEG chains can block the binding of the targeting moiety. Here, specific interactions between an antihapten antibody (clone 734, specific for the DTPA-indium complex and DTPA-indium-tagged liposomes were characterized by surface plasmon resonance (SPR. Non-PEGylated liposomes fused on CM5 chips whereas PEGylated liposomes did not. By contrast, both PEGylated and non-PEGylated liposomes attached to L1 chips without fusion. SPR binding kinetics showed that, in the absence of PEG, the antibody binds the hapten at the surface of lipid bilayers with the affinity of the soluble hapten. The incorporation of PEGylated lipids hinders antibody binding to extents depending on PEGylated lipid fraction and PEG molecular weight. SPR on immobilized liposomes thus appears as a useful technique to optimize formulations of liposomes for targeted therapy.

  11. 分子印迹结合 SPR 技术检测蔬菜中农药残留%Pesticide Residues Detection in Vegetables Using Molecular Imprinting Technique Combined with SPR

    Institute of Scientific and Technical Information of China (English)

    魏传平

    2015-01-01

    The technology of surface plasmon resonance ( SPR) is a kind of physical optical phenomena generated by the optical coupling of metal thin film .By monitoring the changes of SPR , real-time monitoring the intermolecular interaction , has no need of fluorescent labeled .Molecular imprinting is a method that can prepare polymer material with predetermined selectivity towards a certain guest molecule .The selective recog-nition of molecularly imprinted polymers to the template molecules is obtained through the "memory effect"of the cavities formed during the polymerization process .This paper is based on the explanation of acephate tem-plate molecularly imprinted membrane ( MIPS ) , and then uses the SPR sensor for residue analysis of pesti-cides in vegetables detection .The satisfactory results were obtained .%表面等离子体共振技术( SPR)是利用金属薄膜的光学耦合产生的一种物理“光学现象”。可通过监测SPR的变化,实时监测分子间的相互作用,具有无需荧光标记等特点。分子印迹技术是制备对某一客体分子具有预定选择性材料的过程,利用合成的聚合物内的空穴对模板分子的“记忆效应”来实现对模板分子的选择性识别。本文是以乙酰甲胺膦为模板分子制备的印迹聚合物膜(MIPS),利用SPR传感器进行检测分析蔬菜中的农药残留,结果表明,此种分子印迹传感器具有较好的印迹效果和选择性能。

  12. Characterisation of Gd{sub 2}O{sub 2}S:Pr phosphor screens for water window X-ray detection

    Energy Technology Data Exchange (ETDEWEB)

    Fern, George [Wolfson Centre for Materials Processing, Brunel University, Uxbridge, London, UB8 3PH (United Kingdom)], E-mail: george.fern@brunel.ac.uk; Ireland, Terry; Silver, Jack; Withnall, Robert [Wolfson Centre for Materials Processing, Brunel University, Uxbridge, London, UB8 3PH (United Kingdom); Michette, Alan [King' s College London, Department of Physics, Strand, London, WC2R 2LS (United Kingdom)], E-mail: alan.michette@kcl.ac.uk; McFaul, Chris; Pfauntsch, Slawka [King' s College London, Department of Physics, Strand, London, WC2R 2LS (United Kingdom)

    2009-03-01

    A soft X-ray scintillator phosphor (Gd{sub 2}O{sub 2}S:Pr) has been synthesised which is composed of discrete crystals of 120x150 nm average size. This material has been deposited onto a transparent substrate so that it can be used to detect X-rays generated by a laser generated plasma. A photodiode has then been used to quantify the intensity of emission by the phosphor slide. This nano-crystalline Gd{sub 2}O{sub 2}S:Pr phosphor shows greatly enhanced signal over a commercially available phosphor with larger particle size.

  13. Systém pro správu bezpečnostního procesu ve firmě

    OpenAIRE

    Roubal, Petr

    2007-01-01

    Tato bakalářská práce se zabývá zpracováním metodiky pro správu bezpečnostního procesu ve firmě. Tato metodika bude používána jako podklad pro softwarový produkt. Jsou zde popsaná i témeta s bezpečnostním procesem uzce související. Těmito tématy jsou: změna a změnové řízení, riziko a řízení rizik, procesy, modelování procesů a řízení procesů. Metodika je popsána pomoci popsani procesů. This bachelor`s thesis is concerned with methodology for Company Security Processes Administration System...

  14. Site-directed antibody immobilization using a protein A-gold binding domain fusion protein for enhanced SPR immunosensing.

    Science.gov (United States)

    de Juan-Franco, Elena; Caruz, Antonio; Pedrajas, J R; Lechuga, Laura M

    2013-04-07

    We have implemented a novel strategy for the oriented immobilization of antibodies onto a gold surface based on the use of a fusion protein, the protein A-gold binding domain (PAG). PAG consists of a gold binding peptide (GBP) coupled to the immunoglobulin-binding domains of staphylococcal protein A. This fusion protein provides an easy and fast oriented immobilization of antibodies preserving its native structure, while leaving the antigen binding sites (Fab) freely exposed. Using this immobilization strategy, we have demonstrated the performance of the immunosensing of the human Growth Hormone by SPR. A limit of detection of 90 ng mL(-1) was obtained with an inter-chip variability lower than 7%. The comparison of this method with other strategies for the direct immobilization of antibodies over gold surfaces has showed the enhanced sensitivity provided by the PAG approach.

  15. Språkfagenes fremtid i et organisasjonsteoretisk perspektiv. Er det liv laga for fremmedspråk?

    Directory of Open Access Journals (Sweden)

    Annelise Brox Larsen

    2014-10-01

    Full Text Available Denne artikkelen drøfter ulike fremtidsscenarier for organiseringen av engelsk- og fremmedspråkopplæringen i den norske skolen. Med bakgrunn i organisasjonsteoretisk translasjonsteori og randomiserte studier av et utvalg nasjonal og internasjonal styrings- og praksisdokumentasjon gis det en sannsynlighetsvurdering av hva vi kan forvente oss i Norge. Selv om det tilsynelatende ser ut som om mange er enige om at vi skal ha mer og bedre språkundervisning så kan vi i realiteten ende opp med mindre og dårligere undervisning fordi vi introduserer andre fremmedspråk for sent, fordi vi lar det være et valgfag og fordi vi ikke innfører CLIL som en obligatorisk arbeidsform.

  16. Numerical simulation of a metal diffraction grating-based SPR sensor with a water-immersion lens

    Science.gov (United States)

    Ichihashi, Kouki; Iwata, Tetsuo

    2017-09-01

    We describe a metal diffraction grating-based surface plasmon resonance sensor (G-SPRS) that operates in a differential mode, the angular sensitivity of which would be 4.8 times that of a conventional G-SPRS and over twice that of a prism-based SPRS. Because the objective is to analyze sample solutions using our G-SPRS, we plan to employ a water-immersion cylindrical lens to obtain simultaneously an angular spectrum of wide range. This G-SPRS enables us to observe two SPR dips corresponding to ±1st-order diffracted light in differential mode and further to generate them symmetrically at relatively large incident angles. We present simulation results and a practical optical design emphasizing its sensitivity in measurement, and thereby the significant advantage of its rigorous design.

  17. 基于光导毛细管器件的 SPR生物传感器及 IgG检测%SPR biosensor based on light guiding capillary element and IgG detection

    Institute of Scientific and Technical Information of China (English)

    刘强; 李明洋; 程昉; 刘云; 刘秀鑫; 彭伟

    2015-01-01

    光纤表面等离子体共振(surface plasmon resonance,SPR)传感技术具有免标记、灵敏度高、体积小、成本低等优点.在传统的光纤 SPR 传感器件基础上设计制造了光导毛细管SPR 传感器件,利用自行研制的波长调制式 SPR 传感装置,对器件灵敏度、分辨率和检测精密度等方面的性能进行了评价.相比光纤 SPR 传感器件,光导毛细管 SPR 传感器件产生更显著的 SPR 效应,提高系统分辨率达9.6×10-6 RIU(折射率单位).应用光导毛细管 SPR 传感器件可进行 IgG 快速、定量检测,检测线性范围受传感区固定的蛋白 A 疏密程度影响,其中0.01 mg/mL 蛋白 A 修饰条件下检测线性范围是0.33~1.53μmol/L,0.10 mg/mL 蛋白A 修饰条件下检测线性范围是0.20~3.67μmol/L;并进行了蛋白 A-IgG 的解离常数测定, Kd=1.4×10-7 mol/L.相对于现有商业 SPR 仪器,基于光导毛细管器件的 SPR 生物传感器使用成本低、便携性好,具有良好的应用前景.%Fiber-optic surface plasmon resonance (SPR)sensing technology has the advantages of label-free,high sensitivity,miniaturization and low cost,etc..The light guiding capillary SPR sensing element was fabricated on the basis of the fiber-optic SPR sensing element.Using the home-built wavelength-modulated SPR sensing apparatus,their sensitivity,resolution and precision of detection were evaluated.Compared with the fiber-optic SPR sensing element,the light guiding capillary SPR sensing element generates a stronger SPR effect and improves the system resolution (9.6×10 -6 refractive index unit).Using the light guiding capillary SPR sensing element to detect IgG could achieve rapid and quantitative detection.The linear detection range of IgG is affected by the density of protein A immobilized on the sensing area,and under 0.01 mg/mL protein A modified condition is 0.33-1.53 μmol/L,under 0.10 mg/mL protein A modified condition is 0.20-3.67 μmol/L.Moreover,the dissociation

  18. Evaluation of agglutination strength by a flow-induced cell movement assay based surface plasmon resonance (SPR) technique.

    Science.gov (United States)

    Sudprasert, Krisda; Peungthum, Patjaree; Vongsakulyanon, Apirom; Amarit, Ratthasart; Somboonkaew, Armote; Sutapun, Boonsong; Kitpoka, Pimpun; Kunakorn, Mongkol; Srikhirin, Toemsak

    2015-02-07

    A flow-induced cell movement assay combined with a surface plasmon resonance (SPR) technique was developed to quantify the agglutination strength, derived from the standard tube-agglutination test. Red blood cells (RBCs), based on the ABO blood group system, were specifically captured by anti-A and/or anti-B antibodies immobilized on a sensor surface. The agglutination strength corresponds to the amount of antigen-antibody interactions or the strength of RBC adhesion. Under a shear flow, the adherent RBCs were forced to move out of the region of interest with different average cell velocities (vc) depending upon the adhesion strength and wall shear stress (WSS). That is, a higher adhesion strength (higher agglutination strength) or lower WSS represents a lower vc or vice versa. In this work, the agglutination strength was derived from the vc that was calculated from the time derivative of the relative SPR signal by using a simple model of cell movement response, whose validity was verified. The vc values of different samples were correlated with their agglutination strengths at a given WSS and antibody surface density. The vc decreased as the agglutination strength increased, which can be considered as a linear regression. The coefficient of variation of the calculated vc decreased to 0.1 as vc increased to 30 μm min(-1). The sensitivity of this assay can be controlled by optimizing the antibody surface density or the WSS. This assay has the capability to resolve the antigen density of A1 and B RBCs from that of A1B RBCs.

  19. A microfluidic-based hybrid SPR/molecular imaging biosensor for the multiplexed detection of foodborne pathogens

    Science.gov (United States)

    Zordan, Michael D.; Grafton, Meggie M. G.; Acharya, Ghanashyam; Reece, Lisa M.; Aronson, Arthur I.; Park, Kinam; Leary, James F.

    2009-02-01

    It is important to screen our food supply for pathogen contaminations. Current methods to screen for bacterial contamination involve using costly reagents such as antibodies or PCR reagents or time-costly growth in cultures. There is need for portable, real-time, multiplex pathogen detection technology that can predict the safety of food where it is produced or distributed. Surface plasmon resonance (SPR) imaging is a sensitive, label-free method that can detect the binding of an analyte to a surface due to changes in refractive index that occur upon binding. It can be used for label-free detection of the presence of potential pathogens. Simultaneous fluorescence molecular imaging on the other side of the biochip can be used to ascertain pathogen status or functional state which may affect its potential danger to humans or animals. We are designing and testing hybrid microfluidic biochips to detect multiple pathogens using a combination of SPRI and fluorescence imaging. The device consists of an array of gold spots, each functionalized with a peptide targeting a specific pathogen. This peptide biosensor array is enclosed by a PDMS microfluidic flow chamber that delivers a magnetically concentrated sample to be tested. An SPR image is taken from the bottom of the biochip. Image analysis is used to quantify the amount of pathogen (both live and dead) bound to each spot. Since PDMS is very transmissive to visible light, an epi-fluorescence image is taken from the top of the biochip. Fluorescence imaging determines the live:dead ratio of each pathogen using an inexpensive SYTO 9(R)-Propidium Iodide assay. The volume of sample that the biochip can analyze is small, so possible pathogens are pre-concentrated using immunomagnetic separation. Functionalized magnetic particles are bound to pathogens present in the sample, and a magnet is used to separate them from the bulk fluid.

  20. Passive Fluidic Chip Composed of Integrated Vertical Capillary Tubes Developed for On-Site SPR Immunoassay Analysis Targeting Real Samples

    Directory of Open Access Journals (Sweden)

    Tsuneyuki Haga

    2012-05-01

    Full Text Available We have successfully developed a surface plasmon resonance (SPR measurement system for the on-site immunoassay of real samples. The system is composed of a portable SPR instrument (290 mm(W x 160 mm(D x 120 mm(H and a microfluidic immunoassay chip (16 mm(W x 16 mm(D x 4 mm(H that needs no external pump system. An integrated vertical capillary tube functions as a large volume (150 μL passive pump and a waste reservoir that has sufficient capacity for several refill operations. An immunoassay was carried out that employed the direct injection of a buffer and a test sample in sequence into a microfluidic chip that included 9 antibody bands and 10 reference reagent bands immobilized in the flow channel. By subtracting a reliable averaged reference sensorgram from the antibody, we effectively reduced the influence of the non-specific binding, and then our chip successfully detected the specific binding of spiked IgG in non-homogeneous milk. IgG is a model antigen that is certain not to be present in non-homogeneous milk, and non-homogeneous milk is a model of real sample that includes many interfering foreign substances that induce non-specific binding. The direct injection of a real sample with no pretreatment enabled us to complete the entire immunoassay in several minutes. This ease of operation and short measuring time are acceptable for on-site agricultural, environmentaland medical testing.

  1. SPR investigations of the formation of intermediate layer of the immunosensor bioselective element based on the recombinant Staphylococcal protein A

    Directory of Open Access Journals (Sweden)

    Rachkov A. E.

    2015-08-01

    Full Text Available Aim. To investigate the formation of an intermediate layer of the immunosensor bioselective element based on the recombinant protein A from Staphylococcus aureus with cysteine residue (SPA-Cys and its interactions with human IgG using the SPR spectrometer «Plasmon». Methods. The activity of the immune components applied was tested by ELISA. The spectrometry of surface plasmon resonance was used for studying protein immobilization on a gold sensor surface and interactions between the immobilized SPA-Cys and human immunoglobulin. Results. A direct dependence of the sensor response on the concentration of SPA-Cys in the range of 0.2 to 2 µM at its immobilization was demonstrated. The efficiency of blocking nonspecific adsorption sites on the sensor surface with milk proteins and the direct dependence of the sensor response on IgG concentration and surface density of immobilized SPA-Cys were shown. Fitting the experimental data to a Langmuir plot yields a Kd value for SPA-Cys/IgG binding 8.5 ± 0.7× 10-8 M (Ka = 1.2 ± 0.1× 107 M–1. The determined equilibrium binding constant indicates a quite strong interaction and its value is consistent with the literature data. Conclusions. A successful immobilization of SPA-Cys on a gold surface of the SPR spectrometer while preserving its high immunoglobulin-binding activity, selectivity and stability of the sensor response confirms the efficiency of SPA-Cys as an intermediate component for the creation of the immunosensor bioselective elements.

  2. Comparison of the CPU and memory performance of StatPatternRecognition (SPR) and Toolkit for MultiVariate Analysis (TMVA)

    CERN Document Server

    Palombo, Giulio

    2011-01-01

    High Energy Physics data sets are often characterized by a huge number of events. Therefore, it is extremely important to use statistical packages able to efficiently analyze these unprecedented amounts of data. We compare the performance of the statistical packages StatPatternRecognition (SPR) and Toolkit for MultiVariate Analysis (TMVA). We focus on how CPU time and memory usage of the learning process scale versus data set size. As classifiers, we consider Random Forests, Boosted Decision Trees and Neural Networks. For our tests, we employ a data set widely used in the machine learning community, "Threenorm" data set, as well as data tailored for testing various edge cases. For each data set, we constantly increase its size and check CPU time and memory needed to build the classifiers implemented in SPR and TMVA. We show that SPR is often significantly faster and consumes significantly less memory. For example, the SPR implementation of Random Forest is by an order of magnitude faster and consumes an order...

  3. Analysis of cavern and well stability at the West Hackberry SPR site using a full-dome model.

    Energy Technology Data Exchange (ETDEWEB)

    Sobolik, Steven R. [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)

    2015-08-01

    This report presents computational analyses that simulate the structural response of caverns at the Strategic Petroleum Reserve (SPR) West Hackberry site. The cavern field comprises 22 caverns. Five caverns (6, 7, 8, 9, 11) were acquired from industry and have unusual shapes and a history dating back to 1946. The other 17 caverns (101-117) were leached according to SPR standards in the mid-1980s and have tall cylindrical shapes. The history of the caverns and their shapes are simulated in a three-dimensional geomechanics model of the site that predicts deformations, strains, and stresses. Future leaching scenarios corresponding to oil drawdowns using fresh water are also simulated by increasing the volume of the caverns. Cavern pressures are varied in the model to capture operational practices in the field. The results of the finite element model are interpreted to provide information on the current and future status of subsidence, well integrity, and cavern stability. The most significant results in this report are relevant to Cavern 6. The cavern is shaped like a bowl with a large ceiling span and is in close proximity to Cavern 9. The analyses predict tensile stresses at the edge of the ceiling during repressurization of Cavern 6 following workover conditions. During a workover the cavern is at low pressure to service a well. The wellhead pressures are atmospheric. When the workover is complete, the cavern is repressurized. The resulting elastic stresses are sufficient to cause tension around the edge of the large ceiling span. With time, these stresses relax to a compressive state because of salt creep. However, the potential for salt fracture and propagation exists, particularly towards Cavern 9. With only 200 feet of salt between the caverns, the operational consequences must be examined if the two caverns become connected. A critical time may be during a workover of Cavern 9 in part because of the operational vulnerabilities, but also because dilatant damage

  4. SPR-HPV技术检测宫颈人乳头瘤病毒感染状况的临床分析研究%Clinical analysis and research on infection status of human papillomavirus detected by SPR-HPV technology

    Institute of Scientific and Technical Information of China (English)

    李恬; 唐良萏; 卞度宏; 贾英; 黄新; 张新华

    2013-01-01

    Objective: To understand the infection status of human papillomavirus ( HPV) in female patients from the hospital by SPR - HPV technology. Methods: SPR - HPV technology was used to detect 24 kinds of HPV gene subtypes in cervical exfoliative cells and genital tract secretions of 1 000 women. Results: The total HPV infection rate was 28. 2% (282/1 000) , 22 subtypes were detected; 211 women (21. 1% ) were found with single infection of HPV, including 94 women with abnormal cytological Results: 71 women (7. 1% ) were found with multiple infection of HPV, including 32 women with abnormal cytological Results: there was no statistically significant difference between the women with single infection of HPV and the women with multiple infection of HPV (χ2 = 0. 005 5, P > 0. 05); among 211 women with single infection of HPV, 186 women were found with high risk HPV infection, including 90 women with abnormal cytological results ; 25 women were found with low risk HPV infection, including 4 women with abnormal cytological Results: there was statistically significant difference (χ2 =9.37, P < 0.005) . Among 211 women with single infection of HPV, the top four high risk HPV genotypes were HPV16, HPV58, HPV52, and HPV51, 54 women (25. 29% ) were found with HPV16 infection; HPV single infection was the main type a-mong HPV infection, among 151 women with abnormal cytological results, 94 women were found with single infection of HPV. Conclusion: The infection rate of HPV among women from the hospital was at a middle level compared with other areas in China, single infection and high risk infection were the main type. Appropriate HPV screening should be conducted among the cases receiving opportunistic screening in clinic , and regular follow - up should be performed, which is helpful to prevent and find cervical malignant lesions timely and reduce the damage of cervical malignant lesions to women.%目的:用SPR-HPV技术了解重庆医科大学附属第一医院就诊女性人

  5. Hybrid SPR algorithm to select predictive genes for effectual cancer classification

    OpenAIRE

    2012-01-01

    Designing an automated system for classifying DNA microarray data is an extremely challenging problem because of its high dimension and low amount of sample data. In this paper, a hybrid statistical pattern recognition algorithm is proposed to reduce the dimensionality and select the predictive genes for the classification of cancer. Colon cancer gene expression profiles having 62 samples of 2000 genes were used for the experiment. A gene subset of 6 highly informative genes was selecte...

  6. QUICK identification and SPR validation of signal transducers and activators of transcription 3 (Stat3) interacting proteins.

    Science.gov (United States)

    Zheng, Peng; Zhong, Qiu; Xiong, Qian; Yang, Mingkun; Zhang, Jia; Li, Chongyang; Bi, Li-Jun; Ge, Feng

    2012-01-04

    Signal transducers and activators of transcription 3 (Stat3) has been reported to be involved in the pathogenesis of various human diseases and is constitutively active in human multiple myeloma (MM) U266 cells. The Stat3-regulated mechanisms involved in these processes, however, are not fully defined. To further understand the regulation of Stat3 activity, we performed a systematic proteomic analysis of Stat3 interacting proteins in U266 cells. This analysis, termed quantitative immunoprecipitation combined with knockdown (QUICK), combines RNAi, stable isotope labeling with amino acids in cell culture (SILAC), immunoprecipitation, and quantitative MS. As a result, quantitative mass spectrometry analysis allowed us to distinguish specific Stat3 interacting proteins from background proteins and led to the identification of a total of 38 proteins. Three Stat3 interacting proteins - 14-3-3ζ, PRKCB and Hsp90 - were further confirmed by reciprocal co-immunoprecipitations and surface plasmon resonance (SPR) analysis. Our results therefore not only uncover a number of Stat3 interacting proteins that possess a variety of cellular functions, but also provide new insight into the mechanisms that regulate Stat3 activity and function in MM cells.

  7. A holistic model for coastal flooding using system diagrams and the Source-Pathway-Receptor (SPR concept

    Directory of Open Access Journals (Sweden)

    S. Narayan

    2012-05-01

    Full Text Available Coastal flooding is a problem of increasing relevance in low-lying coastal regions worldwide. In addition to the anticipated increase in likelihood and magnitude of coastal floods due to climate change, there is rapid growth in coastal assets and infrastructure. Sustainable and integrated coastal flood management over large areas and varying coastline types cannot be simply treated as local combinations of flood defences and floodplains. Rather, a system level analysis of floodplains is required to structure the problem as a first step before applying quantitative models. In this paper such a model is developed using system diagrams and the Source-Pathway-Receptor (SPR concept, to structure our understanding of large and complex coastal flood systems. A graphical systems model is proposed for the assessment of coastal flood systems with regard to individual elements and their topological relationships. Two examples are discussed – a unidirectional model for a large-scale flood system, and a multi-directional model for a smaller-scale system, both based on the Western Scheldt estuary. The models help to develop a comprehensive understanding of system elements and their relationships and provide a holistic overview of the coastal flood system. The approach shows that a system level analysis of floodplains is more effective than simple topographic maps when conveying complex information. The models are shown to be useful as an apriori approach for making the assumptions about flood mechanisms explicit and for informing inputs to numerical models.

  8. Sensitive and rapid detection of aflatoxin M1 in milk utilizing enhanced SPR and p(HEMA) brushes.

    Science.gov (United States)

    Karczmarczyk, Aleksandra; Dubiak-Szepietowska, Monika; Vorobii, Mariia; Rodriguez-Emmenegger, Cesar; Dostálek, Jakub; Feller, Karl-Heinz

    2016-07-15

    The rapid and sensitive detection of aflatoxin M1 (AFM1) in milk by using surface plasmon resonance (SPR) biosensor is reported. This low molecular weight mycotoxin is analyzed using an indirect competitive immunoassay that is amplified by secondary antibodies conjugated with Au nanoparticles. In order to prevent fouling on the sensor surface by the constituents present in analyzed milk samples, an interface with poly(2-hydroxyethyl methacrylate) p(HEMA) brush was employed. The study presents a comparison of performance characteristics of p(HEMA)-based sensor with a regularly used polyethylene glycol-based architecture relying on mixed thiol self-assembled monolayer. Both sensors are characterized in terms of surface mass density of immobilized AFM1 conjugate as well as affinity bound primary and secondary antibodies. The efficiency of the amplification strategy based on Au nanoparticle is discussed. The biosensor allowed for highly sensitive detection of AFM1 in milk with a limit of detection (LOD) as low as 18pgmL(-1) with the analysis time of 55min.

  9. Integrating nanohybrid membranes of reduced graphene oxide: chitosan: silica sol gel with fiber optic SPR for caffeine detection.

    Science.gov (United States)

    Kant, Ravi; Tabassum, Rana; Gupta, Banshi D

    2017-05-12

    Caffeine is the most popular psychoactive drug consumed in the world for improving alertness and enhancing wakefulness. However, caffeine consumption beyond limits can result in lot of physiological complications in human beings. In this work, we report a novel detection scheme for caffeine integrating nanohybrid membranes of reduced graphene oxide (rGO) in chitosan modified silica sol gel (rGO: chitosan: silica sol gel) with fiber optic surface plasmon resonance. The chemically synthesized nanohybrid membrane forming the sensing route has been dip coated over silver coated unclad central portion of an optical fiber. The sensor works on the mechanism of modification of dielectric function of sensing layer on exposure to analyte solution which is manifested in terms of red shift in resonance wavelength. The concentration of rGO in polymer network of chitosan and silica sol gel and dipping time of the silver coated probe in the solution of nanohybrid membrane have been optimized to extricate the supreme performance of the sensor. The optimized sensing probe possesses a reasonably good sensitivity and follows an exponentially declining trend within the entire investigating range of caffeine concentration. The sensor boasts of an unparalleled limit of detection value of 1.994 nM and works well in concentration range of 0-500 nM with a response time of 16 s. The impeccable sensor methodology adopted in this work combining fiber optic SPR with nanotechnology furnishes a novel perspective for caffeine determination in commercial foodstuffs and biological fluids.

  10. Integrating nanohybrid membranes of reduced graphene oxide: chitosan: silica sol gel with fiber optic SPR for caffeine detection

    Science.gov (United States)

    Kant, Ravi; Tabassum, Rana; Gupta, Banshi D.

    2017-05-01

    Caffeine is the most popular psychoactive drug consumed in the world for improving alertness and enhancing wakefulness. However, caffeine consumption beyond limits can result in lot of physiological complications in human beings. In this work, we report a novel detection scheme for caffeine integrating nanohybrid membranes of reduced graphene oxide (rGO) in chitosan modified silica sol gel (rGO: chitosan: silica sol gel) with fiber optic surface plasmon resonance. The chemically synthesized nanohybrid membrane forming the sensing route has been dip coated over silver coated unclad central portion of an optical fiber. The sensor works on the mechanism of modification of dielectric function of sensing layer on exposure to analyte solution which is manifested in terms of red shift in resonance wavelength. The concentration of rGO in polymer network of chitosan and silica sol gel and dipping time of the silver coated probe in the solution of nanohybrid membrane have been optimized to extricate the supreme performance of the sensor. The optimized sensing probe possesses a reasonably good sensitivity and follows an exponentially declining trend within the entire investigating range of caffeine concentration. The sensor boasts of an unparalleled limit of detection value of 1.994 nM and works well in concentration range of 0-500 nM with a response time of 16 s. The impeccable sensor methodology adopted in this work combining fiber optic SPR with nanotechnology furnishes a novel perspective for caffeine determination in commercial foodstuffs and biological fluids.

  11. Fabrication and Characterization of a SPR Based Fiber Optic Sensor for the Detection of Chlorine Gas Using Silver and Zinc Oxide

    Directory of Open Access Journals (Sweden)

    Sruthi P. Usha

    2015-04-01

    Full Text Available A fiber optic chlorine gas sensor working on surface plasmon resonance (SPR technique fabricated using coatings of silver and zinc oxide films over unclad core of the optical fiber is reported. The sensor probe is characterized using wavelength interrogation and recording SPR spectra for different concentrations of chlorine gas around the probe. A red shift is observed in the resonance wavelength on increasing the concentration of the chlorine gas. The thickness of the zinc oxide film is optimized to achieve the maximum sensitivity of the sensor. In addition to wavelength interrogation, the sensor can also work on intensity modulation. The selectivity of the sensor towards chlorine gas is verified by carrying out measurements for different gases. The sensor has various advantages such as better sensitivity, good selectivity, reusability, fast response, low cost, capability of online monitoring and remote sensing.

  12. A Complete Optical Sensor System Based on a POF-SPR Platform and a Thermo-Stabilized Flow Cell for Biochemical Applications.

    Science.gov (United States)

    Cennamo, Nunzio; Chiavaioli, Francesco; Trono, Cosimo; Tombelli, Sara; Giannetti, Ambra; Baldini, Francesco; Zeni, Luigi

    2016-02-04

    An optical sensor platform based on surface plasmon resonance (SPR) in a plastic optical fiber (POF) integrated into a thermo-stabilized flow cell for biochemical sensing applications is proposed. This device has been realized and experimentally tested by using a classic receptor-analyte assay. For this purpose, the gold surface of the POF was chemically modified through the formation of a self-assembling monolayer. The surface robustness of the POF-SPR platform has been tested for the first time thanks to the flow cell. The experimental results show that the proposed device can be successfully used for label-free biochemical sensing. The final goal of this work is to achieve a complete, small-size, simple to use and low cost optical sensor system. The whole system with the flow cell and the optical sensor are extensively described, together with the experimental results obtained with an immunoglobulin G (IgG)/anti-IgG assay.

  13. A Complete Optical Sensor System Based on a POF-SPR Platform and a Thermo-Stabilized Flow Cell for Biochemical Applications

    Directory of Open Access Journals (Sweden)

    Nunzio Cennamo

    2016-02-01

    Full Text Available An optical sensor platform based on surface plasmon resonance (SPR in a plastic optical fiber (POF integrated into a thermo-stabilized flow cell for biochemical sensing applications is proposed. This device has been realized and experimentally tested by using a classic receptor-analyte assay. For this purpose, the gold surface of the POF was chemically modified through the formation of a self-assembling monolayer. The surface robustness of the POF-SPR platform has been tested for the first time thanks to the flow cell. The experimental results show that the proposed device can be successfully used for label-free biochemical sensing. The final goal of this work is to achieve a complete, small-size, simple to use and low cost optical sensor system. The whole system with the flow cell and the optical sensor are extensively described, together with the experimental results obtained with an immunoglobulin G (IgG/anti-IgG assay.

  14. HAHA--nothing to laugh about. Measuring the immunogenicity (human anti-human antibody response) induced by humanized monoclonal antibodies applying ELISA and SPR technology.

    Science.gov (United States)

    Nechansky, Andreas

    2010-01-05

    Immunogenicity induced by passively applied proteins is a serious issue because it is directly related to the patient's safety. The out-come of an immune reaction to a therapeutic protein can range from transient appearance of antibodies without any clinical significance to severe life threatening conditions. Within this article, enzyme-linked immunosorbent assay (ELISA) and surface plasmon resonance (SPR) methodology to measure immunogenicity are compared and the pros and cons are discussed.

  15. MultiNord (2007-2015): Et nettverk for forskning om språk i heterogene byrom – empiriske og teoretiske hovedlinjer

    DEFF Research Database (Denmark)

    Quist, Pia; Svendsen, Bente Ailin

    2015-01-01

    Formålet med denne artikkelen er med utgangspunkt i nettverket MultiNord å trekke opp empiriske og teoretiske hovedlinjer i forskningen om språk i flerspråklige bymiljøer i Skandinavia. MultiNord ble opprettet i 2007 og består av cirka 60 forskere og interessenter som er (eller har vært) ansatt ved...

  16. Fiber SPR micro-displacement sensor based on Otto structure%基于Otto结构的光纤SPR微位移传感器

    Institute of Scientific and Technical Information of China (English)

    王雁茹; 赵春柳; 康娟

    2016-01-01

    提出一种基于Otto结构的高灵敏度光纤表面等离子体共振(SPR)微位移传感器.通过有限元分析软件COMSOL MultiphysicsULTIPHYSICS对该传感器的传感特性进行了理论数值研究,分析了表面等离子体共振波长与位移变化的关系.

  17. SPR imaging based electronic tongue via landscape images for complex mixture analysis.

    Science.gov (United States)

    Genua, Maria; Garçon, Laurie-Amandine; Mounier, Violette; Wehry, Hillary; Buhot, Arnaud; Billon, Martial; Calemczuk, Roberto; Bonnaffé, David; Hou, Yanxia; Livache, Thierry

    2014-12-01

    Electronic noses/tongues (eN/eT) have emerged as promising alternatives for analysis of complex mixtures in the domain of food and beverage quality control. We have recently developed an electronic tongue by combining surface plasmon resonance imaging (SPRi) with an array of non-specific and cross-reactive receptors prepared by simply mixing two small molecules in varying and controlled proportions and allowing the mixtures to self-assemble on the SPRi prism surface. The obtained eT generated novel and unique 2D continuous evolution profiles (CEPs) and 3D continuous evolution landscapes (CELs) based on which the differentiation of complex mixtures such as red wine, beer and milk were successful. The preliminary experiments performed for monitoring the deterioration of UHT milk demonstrated its potential for quality control applications. Furthermore, the eT exhibited good repeatability and stability, capable of operating after a minimum storage period of 5 months.

  18. Identification of a novel mitochondrial protein, short postembryonic roots 1 (SPR1), involved in root development and iron homeostasis in Oryza sativa.

    Science.gov (United States)

    Jia, Liqiang; Wu, Zhongchang; Hao, Xi; Carrie, Chris; Zheng, Libin; Whelan, James; Wu, Yunrong; Wang, Shoufeng; Wu, Ping; Mao, Chuanzao

    2011-02-01

    • A rice mutant, Oryza sativa short postembryonic roots 1 (Osspr1), has been characterized. It has short postembryonic roots, including adventitious and lateral roots, and a lower iron content in its leaves. • OsSPR1 was identified by map-based cloning. It encodes a novel mitochondrial protein with the Armadillo-like repeat domain. • Osspr1 mutants exhibited decreased root cell elongation. The iron content of the mutant shoots was significantly altered compared with that of wild-type shoots. A similar pattern of alteration of manganese and zinc concentrations in shoots was also observed. Complementation of the mutant confirmed that OsSPR1 is involved in post-embryonic root elongation and iron homeostasis in rice. OsSPR1 was found to be ubiquitously expressed in various tissues throughout the plant. The transcript abundance of various genes involved in iron uptake and signaling via both strategies I and II was similar in roots of wild-type and mutant plants, but was higher in the leaves of mutant plants. • Thus, a novel mitochondrial protein that is involved in root elongation and plays a role in metal ion homeostasis has been identified.

  19. Effects of JA Synthesis-Related Genes Spr2 and LePrs on the Resistance to Root-Knot Nematodes in Tomato%茉莉酸合成相关基因Spr2与LePrs在番茄抗根结线虫中的作用

    Institute of Scientific and Technical Information of China (English)

    张立宁; 程继鸿; 杨瑞; 孙中华; 吴春霞; 王绍辉

    2011-01-01

    [目的]研究茉莉酸合成相关基因Spr2与LePrs对番茄抗根结线虫的影响,探讨茉莉酸在番茄抗根结线虫病中的作用.[方法]以番茄茉莉酸合成突变体spr2、茉莉酸过量表达转基因番茄35S(::)PS及野生型CM为试材,研究外源喷施茉莉酸甲酯及不同番茄试材相互嫁接对接种根结线虫后番茄根结数、蛋白质酶抑制剂PI-II表达及Spr2、LePrs基因转录水平的影响.[结果]番茄茉莉酸合成突变体spr2较野生型与茉莉酸过量表达转基因番茄35S::PS容易感染根结线虫,外源喷施茉莉酸甲酯降低了番茄根结数.以茉莉酸过量表达转基因番茄35S(::)PS为砧木,可以提高嫁接植株对根结线虫的抵抗能力.[结论]Spr2突变降低了番茄对根结线虫的抗性, LePrs过量表达可以提高植株对根结线虫的抗性.因此,茉莉酸在番茄抗根结线虫病中起到了一定的作用.%[Objective] Spr2 and LePrs were related with JA synthesis, the effects of the two genes on the response of tomato to root-knot nematodes were studied. [Method] In this paper, JA biosynthetic mutant (spr2 plants) and JA-overexpression transgenic plants (35S::PS plants) of tomato as well as wild tomato species (CM) were used, and the effects of MeJA sprays and grafting on root-knot numbers, P1-II content and Spr2, LePrs transcription levels, before and after inoculating root-knot nematodes, were analyzed. [Result] Compared with CM and 35S::PS plants, spr2 plants was more susceptible to root-knot nematodes. Spraying MeJA on tomato leaves could reduce root-knot numbers. Using 35S::PS plants as stock improved grafting tomato resistance to root-knot nematodes. [Conclusion] Resistance to nematodes in JA biosynthetic mutant (with mutant Spr2) was reduced, while in JA-overexpression transgenic tomato (with over-expressing LePrs) it was increased. So Jasmonic acid played a role in the response of tomato to root-knot nematodes.

  20. Enhanced sensitivity of self-assembled-monolayer-based SPR immunosensor for detection of benzaldehyde using a single-step multi-sandwich immunoassay.

    Science.gov (United States)

    Gobi, K Vengatajalabathy; Matsumoto, Kiyoshi; Toko, Kiyoshi; Ikezaki, Hidekazu; Miura, Norio

    2007-04-01

    This paper describes the fabrication and sensing characteristics of a self-assembled monolayer (SAM)-based surface plasmon resonance (SPR) immunosensor for detection of benzaldehyde (BZ). The functional sensing surface was fabricated by the immobilization of a benzaldehyde-ovalbumin conjugate (BZ-OVA) on Au-thiolate SAMs containing carboxyl end groups. Covalent binding of BZ-OVA on SAM was found to be dependent on the composition of the base SAM, and it is improved very much with the use of a mixed monolayer strategy. Based on SPR angle measurements, the functional sensor surface is established as a compact monolayer of BZ-OVA bound on the mixed SAM. The BZ-OVA-bound sensor surface undergoes immunoaffinity binding with anti-benzaldehyde antibody (BZ-Ab) selectively. An indirect inhibition immunoassay principle has been applied, in which analyte benzaldehyde solution was incubated with an optimal concentration of BZ-Ab for 5 min and injected over the sensor chip. Analyte benzaldehyde undergoes immunoreaction with BZ-Ab and makes it inactive for binding to BZ-OVA on the sensor chip. As a result, the SPR angle response decreases with an increase in the concentration of benzaldehyde. The fabricated immunosensor demonstrates a low detection limit (LDL) of 50 ppt (pg mL(-1)) with a response time of 5 min. Antibodies bound to the sensor chip during an immunoassay could be detached by a brief exposure to acidic pepsin. With this surface regeneration, reusability of the same sensor chip for as many as 30 determination cycles has been established. Sensitivity has been enhanced further with the application of an additional single-step multi-sandwich immunoassay step, in which the BZ-Ab bound to the sensor chip was treated with a mixture of biotin-labeled secondary antibody, streptavidin and biotin-bovine serum albumin (Bio-BSA) conjugate. With this approach, the SPR sensor signal increased by ca. 12 times and the low detection limit improved to 5 ppt with a total response

  1. Geomechanical testing of MRIG-9 core for the potential SPR siting at the Richton salt dome.

    Energy Technology Data Exchange (ETDEWEB)

    Dunn, Dennis P.; Broome, Scott Thomas; Bronowski, David R.; Bauer, Stephen J.; Hofer, John H.

    2009-02-01

    A laboratory testing program was developed to examine the mechanical behavior of salt from the Richton salt dome. The resulting information is intended for use in design and evaluation of a proposed Strategic Petroleum Reserve storage facility in that dome. Core obtained from the drill hole MRIG-9 was obtained from the Texas Bureau of Economic Geology. Mechanical properties testing included: (1) acoustic velocity wave measurements; (2) indirect tensile strength tests; (3) unconfined compressive strength tests; (4) ambient temperature quasi-static triaxial compression tests to evaluate dilational stress states at confining pressures of 725, 1450, 2175, and 2900 psi; and (5) confined triaxial creep experiments to evaluate the time-dependent behavior of the salt at axial stress differences of 4000 psi, 3500 psi, 3000 psi, 2175 psi and 2000 psi at 55 C and 4000 psi at 35 C, all at a constant confining pressure of 4000 psi. All comments, inferences, discussions of the Richton characterization and analysis are caveated by the small number of tests. Additional core and testing from a deeper well located at the proposed site is planned. The Richton rock salt is generally inhomogeneous as expressed by the density and velocity measurements with depth. In fact, we treated the salt as two populations, one clean and relatively pure (> 98% halite), the other salt with abundant (at times) anhydrite. The density has been related to the insoluble content. The limited mechanical testing completed has allowed us to conclude that the dilatational criteria are distinct for the halite-rich and other salts, and that the dilation criteria are pressure dependent. The indirect tensile strengths and unconfined compressive strengths determined are consistently lower than other coastal domal salts. The steady-state-only creep model being developed suggests that Richton salt is intermediate in creep resistance when compared to other domal and bedded salts. The results of the study provide only

  2. Real-time monitoring of mycobacterium genomic DNA with target-primed rolling circle amplification by a Au nanoparticle-embedded SPR biosensor.

    Science.gov (United States)

    Xiang, Yang; Zhu, Xiaoyan; Huang, Qing; Zheng, Junsong; Fu, Weiling

    2015-04-15

    In this study, we developed a surface plasmon resonance (SPR) DNA biosensor array based on target-primed rolling circle amplification (RCA) for isothermal and rapid detection of two pathogenic mycobacteria, Mycobacterium tuberculosis complex (MTBC) and Mycobacterium avium complex (MAC).The species-specific padlock probe (PLP) was designed to target the sequence in 16S-23S rRNA gene internal transcribed spacer (ITS). After ligation, the circularized PLP could be primed by the target sequence to initial RCA. The RCA performed simultaneously with the cleavage reaction to produce small fragments of single strand DNA which immediately hybridized with the probe immobilized on the sensor chip without denaturation. This process caused SPR angle changes on the chip surface, which made the detection for analysis from the solution achievable, and dynamic real-time RCA monitoring of mycobacterium possible. Besides, Au nanoparticles (AuNPs) were directly assembled onto the surface of the sensor chip via hexanedithiol (HDT) for the enhancement of sensitivity as a label-free detection system. Experimental results show that the signal enhancement by the target-primed RCA together with AuNPs-embedded surface caused at least10-fold increased sensitivity as compared with conventional RCA on bare SPR chip method. Within 40min amplification duration as low as 20amol of synthetic targets and 10(4)CFUmL(-1) of genomic DNA from clinical samples can be detected. The proposed method not only provides a simple design idea for liquid-phase amplification monitoring, but also apply it in clinical pathogen detection, which holds great promise in ultrasensitive bioassay in the future.

  3. SPR Detection and Discrimination of the Oligonucleotides Related to the Normal and the Hybrid bcr-abl Genes by Two Stringency Control Strategies

    Science.gov (United States)

    Matsishin, M. J.; Ushenin, Iu. V.; Rachkov, A. E.; Solatkin, A. P.

    2016-01-01

    In this study, we applied two stringency control strategies for surface plasmon resonance (SPR) detection of DNA hybridization and discrimination of completely and partially complementary 24-mer sequences. These sequences are specific to the human normal bcr and the hybrid bcr-abl genes, protein products of which are responsible for some leukemia. SPR sensors based on resonance phenomena in nanoscale gold films are well suited for label-free, real-time investigations of the macromolecule interactions. Thermodynamic parameters obtained using the web server DINAMelt allowed supposing the possibility for realization (a) stringency control based on the ionic strength of the hybridization buffer and (b) stringency control based on the temperature elevation. The first one resulted in that the discrimination index of completely complementary and partially complementary oligonucleotides depending on the target concentration varied from 1.3 to 1.8 in 2 × SSC and from 2.0 to 2.9 in 0.5 × SSC. For implementation of the second stringency control strategy, SPR spectrometer measuring flow cell with built-in high-precision temperature control and regulation as well as corresponding software was created. It is shown that the duplexes formed by the immobilized probes mod-Ph and completely complementary oligonucleotides P1 remained without significant changes until ~50 °C, while the duplexes formed with partially complementary oligonucleotide Bcrex14 almost entirely disrupted at 40 °C. Thus, the absolutely effective thermodiscrimination of this pair of oligonucleotides was achieved in this temperature range (40-50 °C).

  4. Protein-Flavonoid Interaction Studies by a Taylor Dispersion Surface Plasmon Resonance (SPR Technique: A Novel Method to Assess Biomolecular Interactions

    Directory of Open Access Journals (Sweden)

    Preejith P. Vachali

    2016-02-01

    Full Text Available Flavonoids are common polyphenolic compounds widely distributed in fruits and vegetables. These pigments have important pharmacological relevance because emerging research suggests possible anti-cancer and anti-inflammatory properties as well other beneficial health effects. These compounds are relatively hydrophobic molecules, suggesting the role of blood transport proteins in their delivery to tissues. In this study, we assess the binding interactions of four flavonoids (kaempferol, luteolin, quercetin, and resveratrol with human serum albumin (HSA, the most abundant protein in the blood, and with glutathione S-transferase pi isoform-1 (GSTP1, an enzyme with well-characterized hydrophobic binding sites that plays an important role in detoxification of xenobiotics with reduced glutathione, using a novel Taylor dispersion surface plasmon resonance (SPR technique. For the first time, HSA sites revealed a high-affinity binding site for flavonoid interactions. Out of the four flavonoids that we examined, quercetin and kaempferol showed the strongest equilibrium binding affinities (KD of 63 ± 0.03 nM and 37 ± 0.07 nM, respectively. GSTP1 displayed lower affinities in the micromolar range towards all of the flavonoids tested. The interactions of flavonoids with HSA and GSTP1 were studied successfully using this novel SPR assay method. The new method is compatible with both kinetic and equilibrium analyses.

  5. A randomized trial and novel SPR technique identifies altered lipoprotein-LDL receptor binding as a mechanism underlying elevated LDL-cholesterol in APOE4s

    Science.gov (United States)

    Calabuig-Navarro, M. V.; Jackson, K. G.; Kemp, C. F.; Leake, D. S.; Walden, C. M.; Lovegrove, J. A.; Minihane, A. M.

    2017-01-01

    At a population level APOE4 carriers (~25% Caucasians) are at higher risk of cardiovascular diseases. The penetrance of genotype is however variable and influenced by dietary fat composition, with the APOE4 allele associated with greater LDL-cholesterol elevation in response to saturated fatty acids (SFA). The etiology of this greater responsiveness is unknown. Here a novel surface plasmon resonance technique (SPR) is developed and used, along with hepatocyte (with the liver being the main organ modulating lipoprotein metabolism and plasma lipid levels) uptake studies to establish the impact of dietary fatty acid composition on, lipoprotein-LDL receptor (LDLR) binding, and hepatocyte uptake, according to APOE genotype status. In men prospectively recruited according to APOE genotype (APOE3/3 common genotype, or APOE3/E4), triglyceride-rich lipoproteins (TRLs) were isolated at fasting and 4–6 h following test meals rich in SFA, unsaturated fat and SFA with fish oil. In APOE4s a greater LDLR binding affinity of postprandial TRL after SFA, and lower LDL binding and hepatocyte internalization, provide mechanisms for the greater LDL-cholesterol raising effect. The SPR technique developed may be used for the future study of the impact of genotype, and physiological and behavioral variables on lipoprotein metabolism. Trial registration number NCT01522482. PMID:28276521

  6. 基于LabVIEW搭建光纤SPR虚拟仿真设计平台%To Build the Optic Fiber SPR Virtual Simulation Design Platform Based on LabVIEW

    Institute of Scientific and Technical Information of China (English)

    汪余聪; 林鈺城

    2016-01-01

    The surface plasmon resonance (SPR) based optical fiber sensor is a novel sensor, which takes optical fiber core as a substrate to excite SPR effect. This paper demonstrates a virtual simulation platform for design of optical fiber SPR devices on the basis of LabVIEW, and makes theoretical calculations according to material parameters and component architecture, which can change the size and material parameters in situ to understand the impact on SPR effect, and furthermore to find the optimum parameters. Finally, based on side-polished PMMA plastic optical fiber, the simulation shows that 40nm silver-plated thin film on the grinding surface results in a better performance of optic fiber SPR sensor. In terms of the optimum parameters of the sensor, the relationship between the resonance wavelength and the refractive index of sampling solution can be obtained.%光纤表面等离子体共振(Surface plasmon resonance, SPR)传感器是一种将光纤纤芯作为激发SPR效应基体的新型传感器。本篇论文基于 LabVIEW 搭建虚拟仿真设计平台,依照材料参数与组件结构做理论计算,可即时改变尺寸及材料参数,了解SPR组件参数对感测效果的影响,找到最佳参数。以PMMA 塑料光纤虚拟仿真结果显示,镀银薄膜40nm于光纤研磨表面上,能够制造出一个较佳的光纤 SPR传感器;最后,以不同折射率的待测物进行仿真计算,得到共振波长与折射率关系。

  7. SPR检测器在流动分析系统的应用%Application of SPR detector for flow analysis system

    Institute of Scientific and Technical Information of China (English)

    陈惠滨; 王晓萍; 吴宏伟; 朱岩; 郦雅平

    2011-01-01

    为实现通用性强、样品消耗小、成本低新型检测器的开发,设计一种应用于流动分析体系的表面等离子共振(SPR)检测器.以3种糖的标准溶液为样品,开展检测器性能指标测试,得到相对标准偏差(RSD)值在1.3%以下,D-木糖、蔗糖、葡萄糖的检测下限分别为92、123、132 ng,在最低检测限至10 000 mg/L的检测范围内,线性拟合相关系数大于0.999.将SPR检测器作为液相色谱系统的检测器,测得分离后标准样品RSD值在2.3%以下,蔗糖和半乳糖的检测下限分别为6、9μg.结果表明,SPR检测器对于紫外检测或荧光检测不敏感,或者需要通过衍生化才可进行测量的成分,具有优势.%To develop a novel detector with characteristics of versatility, low sample consumption, and inexpensiveness, surface plasma resonance (SPR) detector was designed for flow analysis system. Its performance was evaluated using 3 glucide solutions.. Results showed that the RSD values were below 1. 3%, and the detection limits of D-xylose, sucrose and glucose were 92, 123, and 132 ng, respectively. The linearities of three components were excellent between detection limits and 10000 mg/L with correlation coefficients above 0. 999. The SPR detector was applied to the liquid chromatography system. The RSD values were below 2. 3% for separated samples with standard concentration solutions, it could detect the sucrose and galactose as low as 6 and 9 μg, respectively. It indicates that the detector is preponderant to detect materials insensitive to the ultraviolet detection or fluorescence detection, or which only can be detected by derivatization.

  8. Research status and application prospects of SPR technology%SPR自冲铆接技术研究现状及应用前景

    Institute of Scientific and Technical Information of China (English)

    吴小丹; 王敏; 孔谅; 王大明

    2016-01-01

    SPR(Self-Piercing Riveting)自冲铆接技术作为一种新型连接工艺,广泛应用于汽车车身制造中.阐明SPR工艺研究的难点和铆接的变形过程,归纳总结国内外关于SPR的相关研究现状,探讨接头质量的评价指标及方法,探究未来SPR工艺研究的方向和研究重点,并列举SPR工艺在汽车工业与其他领域中的应用.

  9. 3-RPS和3-SPR机构的工作空间差异分析%Analysis of Workspace Differences between 3-RPS and 3-SPR Mechanisms

    Institute of Scientific and Technical Information of China (English)

    原政

    2015-01-01

    基于螺旋理论分别对3-RPS和3-SPR机构的自由度进行了分析 ,揭示了两机构由于动平台和静平台的倒置引起的运动转轴的位置变化.采用搜索法分别绘制了两机构的工作空间 ,揭示了转轴位置的差异引起的工作空间的不同.%The degrees of freedom of 3-RPS and 3-SPR are analyzed separately based on screw theory ,the changes of the movement shafts caused by the inversion of the moving platform and the fixed platform are pointed out .The workspaces of two mechanisms are mapped by the search method ,which concludes that different movement shaft will lead to the difference of the workspace .

  10. Tuning of Ag-SPR band position in refractive index controlled inorganic-organic hybrid SiO2-PEO-TiO2 films

    Indian Academy of Sciences (India)

    Samar Kumar Medda; Moumita Mitra; Goutam De

    2008-11-01

    Inorganic (silica-titania)-organic (polyethylene oxide) hybrid films with variable refractive index (RI) values were synthesized and Ag nanoparticles were generated in situ inside such hybrid films to develop coloured coatings specially on plastic substrates. The hybrid films and the corresponding Agincorporated films were prepared from sols derived from a mixture of silicon tetraethoxide (STE), 3-(glycidoxypropyl)trimethoxysilane (GPTMS), titanium tetraisopropoxide (TTIP) and silver nitrate following a sol-gel dip-coating method and cured at low temperature (90°C), followed by UV treatment with an energy equivalent to 5.3 ± 0.1 J cm-2. The equivalent SiO2: SiO1.5(CH2)3OCH2CH(CH2)O : TiO2: Ag molar ratios (nominal) of the final cured films are varied in the ranges (67.9-0) : 29.1 : (0-67.9) : 3. The refractive index values of the cured hybrid films were found to be increased systematically from 1.475 to 1.710 with increasing Ti-component. The Ag-SPR peak, in case of silica-polyethylene oxide host (RI = 1.475), observed at 419 nm, gradually red-shifted to 497 nm upon increasing the Ticomponent (equivalent TiO2 content 67.9 mol%; RI = 1.710) of the film. As a consequence, a systematic change of Ag-SPR position yielded yellow, yellowish-orange, orange, brownish-orange and orangish-brown coloured coatings.

  11. A localized and propagating SPR, and molecular imprinting based fiber-optic ascorbic acid sensor using an in situ polymerized polyaniline-Ag nanocomposite

    Science.gov (United States)

    Shrivastav, Anand M.; Usha, Sruthi P.; Gupta, Banshi D.

    2016-08-01

    We report a successful approach for the fabrication and characterization of a fiber-optic sensor for ascorbic acid (AA) detection, using a molecularly imprinted polyaniline-Ag (PANI-Ag) nanocomposite layer based on the combined phenomena of surface plasmon resonance (SPR) and localized SPR (LSPR). The PANI-Ag nanocomposite is synthesized by an in situ polymerization process and AA imprints are prepared on the polymeric composite. The confirmation of the PANI-Ag nanocomposite and AA imprinting is performed using various characterization methods such as x-ray diffraction (XRD), UV-vis, Fourier transform infrared spectroscopy and scanning electron microscopy. From XRD, the size of Ag nanoparticles is analyzed. The absorbance spectra are recorded for samples of different concentrations of AA around the sensing region of the probe. An increase in peak absorbance wavelength with the increase in AA concentration is observed with a linear response for the concentration range from 10-8 M to 10-6 M. The sensor possesses a high sensitivity of 45.1 nm log-1 M near an AA concentration of 10-8 M. The limit of detection (LOD) and limit of quantification of the sensor are found to be 7.383 × 10-11 M and 4.16 × 10-10 M, respectively. The LOD of the sensor is compared to studies reported in the literature and is found to be the lowest. The sensor possesses several other advantages such as cost effectiveness, selectivity, and low response time (<5 s), along with abilities of remote sensing and online monitoring.

  12. 光纤SPR湿敏传感器及其共振光谱特性研究%Research on Optical Fiber SPR Humidity Sensor and Resonance Spectrum Characteristic

    Institute of Scientific and Technical Information of China (English)

    张少华; 曾捷; 孙晓明; 穆昊; 梁大开

    2012-01-01

    A new type of humidity sensor based on optical fiber SPR sensor probe was introduced and manufactured. Firstly the sensitivity of optical fiber SPR sensor probe to environment humidity was studied. On this basis, coating with thin PVA film of different thickness and with capacity of adsorbing water molecule on the surface of optical fiber SPR sensor probe, for the purpose of monitoring relative humidity was proposed. The research shows that the SPR sensor probe coated with double layers of thin PVA film gets better detection result, and humidity measurement sensitivity is 1. 59%/%RH, being improved notably compared with SPR optical fiber probe. The SPR sensor coated with single layer of thin PVA film has relative humidity measuring sensitivity of 2. 411 nm/5/6RH related to resonance wavelength in high humidity area. In addition, the new type of optical fiber humidity-sensitive probe with PVA film losing efficiency can be reusable after special treatment%提出并研制了基于光纤SPR传感探针的新型湿敏传感器.首先研究了光纤SPR传感探针对环境湿度变化的敏感特性,在此基础上提出在光纤SPR传感探针表面增覆不同厚度且具有水分子吸附功能的PVA薄膜来实现环境相对湿度的监测.研究结果表明,增覆双层PVA薄膜的光纤SPR传感探针在高湿区具有较好监测效果,其共振强度对应的相对湿度测量灵敏度达到1.59%/%RH,较光纤SPR探针呈现显著提高.而增覆单层PVA薄膜的光纤SPR传感探针在高湿区共振波长对应的相对湿度监测灵敏度达到2.411nm/%RH.此外所提出的新型光纤SPR湿敏探针在PVA薄膜失效后经过特殊工艺处理仍可重复镀膜使用.

  13. Implementation of a SPR immunosensor for the simultaneous detection of the 22K and 20K hGH isoforms in human serum samples.

    Science.gov (United States)

    de Juan-Franco, Elena; Rodríguez-Frade, J M; Mellado, M; Lechuga, Laura M

    2013-09-30

    We have implemented a Surface Plasmon Resonance (SPR) immunosensor based on a sandwich assay for the simultaneous detection of the two main hGH isoforms, of 22 kDa (22K) and 20 kDa (20K). An oriented-antibody sensor surface specific for both hormone isoforms was assembled by using the biotin-streptavidin system. The immunosensor functionality was checked for the direct detection of the 22K hGH isoform in buffer, which gave high specificity and reproducibility (intra and inter-assay mean coefficients of variation of 8.23% and 9% respectively). The selective determination of the 22K and 20K hGH isoforms in human serum samples in a single assay was possible by using two specific anti-hGH monoclonal antibodies. The detection limit for both hormone isoforms was 0.9 ng mL(-1) and the mean coefficient of variation was below 7.2%. The excellent reproducibility and sensitivity obtained indicate the high performance of this immunosensor for implementing an anti-doping test.

  14. Quantifying protein-protein interactions in the ubiquitin pathway by surface plasmon resonance

    DEFF Research Database (Denmark)

    Hartmann-Petersen, Rasmus; Gordon, Colin

    2005-01-01

    The commercial availability of instruments, such as Biacore, that are capable of monitoring surface plasmon resonance (SPR) has greatly simplified the quantification of protein-protein interactions. Already, this technique has been used for some studies of the ubiquitin-proteasome system. Here we...

  15. Clinic research into the effects of SPR on the sensory function of lower extremities%选择性脊神经后根切断术对下肢感觉功能的近期影响

    Institute of Scientific and Technical Information of China (English)

    曾瑞曦; 张毅; 郭栋; 刘祥厦; 程钢

    2014-01-01

    Objective To explore the reason why the SPR could make the changes of Sensory Function of Lower extremities,and the clinical significance of these changes.Methods From February,2012 to August,2014,24 patients with spastic cerebral palsy who were consistent with the indications of SPR,had normal intelligence development,and express competence,and could cooperate with the test.To test the changes of Sensory Function of lower extremities preoperatively,1 week after SPR,such as haptics,superficial algaesthesis,temperature sensation,cinaesthesia,topesthesia,pallesthesia,deep pain sense,tactile localization sense,two point discrimination and pattern sense.Results There were 6 patients who present with skin paresthesia of lower extremities among the 24 patients after the SPR.Of the 6 patients,4 became normal 2 weeks later,1 became normal 3 weeks later,and the longest 1 became normal 6 weeks later.No instances with permanent sensory deprivation and anaesthesia were found.The proportions of the excised root of spinal nerves during the SPR in the groups with skin anaesthesia of lower extremities and without skin anaesthesia of lower extremities were 0.268 ± 0.049 and 0.193 ± 0.074 (P < 0.05 respectively).The former was significantly higher than the latter.The changes of haptics,cinaesthesia,topesthesia,deep pain sense,tactile localization sense,two point discrimination,pallesthesia preoperatively,1 week after SPR were not significantly different (P > 0.05).There were significant differences in the superficial algaesthesis,pattern sense and temperature sensation preoperatively and postoperatively (P < 0.05).Both of them decreased compared with those of 1 week after SPR.Conclusion The slight damage to sensory function of lower extremities resulted from SPR may not affect the protectant sensory function of the body.The skin anaesthesia of lower extremities is probably concerned with the proportion of the excised root of spinal nerves during the SPR,but not associated

  16. FO-SPR based dextrose sensor using Ag/ZnO nanorods/GOx for insulinoma detection.

    Science.gov (United States)

    Usha, Sruthi P; Shrivastav, Anand M; Gupta, Banshi D

    2016-11-15

    In this piece of work, a fiber optic sensor has been fabricated and characterized using surface plasmon resonance for dextrose sensing. The concentration range used in this study is for diagnosing the cases of hypoglycaemia especially in suppression tests of insulinoma. Insulinoma is a medical case in which the person is recognized being hypoglycaemic with the blood dextrose level falling down to 2.2mM or less. Thus, the sensor has been characterized for the dextrose concentration range of 0 mM-10mM including the cases of normal blood dextrose range. Coatings of silver layer and zinc oxide nanorods have been carried out on the bare core fiber with a dual role of zinc oxide followed by immobilization of glucose oxidase. A three stage optimization procedure has been adopted for the best performance of the sensor. Absorbance spectra have been plotted and peak absorbance wavelengths have been extracted for each concentration chosen along with the sensitivities. The results have been made conclusive with control experiments. The probe has also been tested on sample having blood serum to check the reliability of the sensor. The sensor shows better selectivity and response time along with its real time applications, online monitoring, remote sensing and reusability.

  17. The incidental pulmonary nodule in a child. Part 1: recommendations from the SPR Thoracic Imaging Committee regarding characterization, significance and follow-up

    Energy Technology Data Exchange (ETDEWEB)

    Westra, Sjirk J. [Massachusetts General Hospital, Division of Pediatric Radiology, Boston, MA (United States); Brody, Alan S. [Cincinnati Children' s Hospital Medical Center, Department of Radiology (CH-1), Cincinnati, OH (United States); Mahani, Maryam Ghadimi [University of Michigan Health System, Section of Pediatric Radiology, C. S. Mott Children' s Hospital Department of Radiology, Ann Arbor, MI (United States); Guillerman, R.P. [Texas Children' s Hospital, Department of Radiology, Houston, TX (United States); Hegde, Shilpa V. [Arkansas Children' s Hospital, Department of Radiology, Little Rock, AR (United States); Iyer, Ramesh S. [Seattle Children' s Hospital, Department of Radiology, Seattle, WA (United States); Lee, Edward Y. [Boston Children' s Hospital, Department of Pediatric Radiology, Boston, MA (United States); Newman, Beverley [Lucile Packard Children' s Hospital at Stanford University, Department of Radiology, Stanford, CA (United States); Podberesky, Daniel J. [Nemours Children' s Hospital, Department of Radiology, Orlando, FL (United States); Thacker, Paul G. [Medical University of South Carolina, Department of Radiology, Charleston, SC (United States)

    2015-05-01

    No guidelines are in place for the follow-up and management of pulmonary nodules that are incidentally detected on CT in the pediatric population. The Fleischner guidelines, which were developed for the older adult population, do not apply to children. This review summarizes the evidence collected by the Society for Pediatric Radiology (SPR) Thoracic Imaging Committee in its attempt to develop pediatric-specific guidelines. Small pulmonary opacities can be characterized as linear or as ground-glass or solid nodules. Linear opacities and ground-glass nodules are extremely unlikely to represent an early primary or metastatic malignancy in a child. In our review, we found a virtual absence of reported cases of a primary pulmonary malignancy presenting as an incidentally detected small lung nodule on CT in a healthy immune-competent child. Because of the lack of definitive information on the clinical significance of small lung nodules that are incidentally detected on CT in children, the management of those that do not have the typical characteristics of an intrapulmonary lymph node should be dictated by the clinical history as to possible exposure to infectious agents, the presence of an occult immunodeficiency, the much higher likelihood that the nodule represents a metastasis than a primary lung tumor, and ultimately the individual preference of the child's caregiver. Nodules appearing in children with a history of immune deficiency, malignancy or congenital pulmonary airway malformation should not be considered incidental, and their workup should be dictated by the natural history of these underlying conditions. (orig.)

  18. Influence of stabilizing agent and synthesis temperature on the optical properties of silver nanoparticles as active materials in surface plasmon resonance (SPR) biosensor

    Science.gov (United States)

    Mahmudin, Lufsyi; Suharyadi, Edi; Utomo, Agung Bambang Setio; Abraha, Kamsul

    2016-04-01

    It has been successfully carried out the synthesis of colloidal silver nanoparticles by chemical reduction method. Silver nitrate (AgNO3) was used as metal precursors and trisodium citrate as the reducing agent. In the synthesis process, were varied the stabilizing agent of Polyvinyl Alcohol (PVA) and polyvinylpyrrolidone (PVP) and heating temperature. The formation of silver nanoparticles was observed visually with discoloration (yellowish). The formation and the structure of silver nanoparticles in colloidal solution were further examined through their optical properties by using a UV-Vis spectrometer. The wavelength absorption spectrum of colloidal silver nanoparticles shows that maximum surface plasmon absorption for the trisodium citrate-synthesized nanoparticles was at 429.43 nm for temperature of 90°C. The addition of the stabilizer sharpened spectrum curves and caused red shift in the maximum absorption peak of 429.01 nm and 427.09 nm for PVA and PVP respectively. Meanwhile, the addition of the synthesis temperature also sharpened the maximum surface plasmon absorption band and the red shift the maximum absorption peak of 428.79 nm and 428.58 nm for temperature of 110°C and 120°C respectively. Red shift of the maximum absorption peak indicates a smaller particle size. The maximum surface plasmon absorption band in the range of 427.09 nm to 429.43 nm indicates the presence of spherical or roughly spherical silver nanoparticles and TEM imaging confirmed this shape. TEM imaging results show that the diameter size of the silver nanoparticles range of 10 nm to 60 nm as well as the morphology (crystallites) of silver nanoparticles have spherical geometry with particle distribution which quite dispersive. The dispersibility of nanoparticles such as this could potentially be used as an active material of SPR biosensor.

  19. Tidlig fremmedspråkundervisning på barneskolen – et nødvendig grunnlag for en god nok språkkompetanse i framtiden

    Directory of Open Access Journals (Sweden)

    Beate Lindemann

    2014-09-01

    Full Text Available I det siste tiåret har det vært gjennomført to forsøk med såkalt tidlig start på 2. fremmedspråk i Norge. De deltagende barneskolene tilbød i disse forsøkene undervisning i et 2. fremmedspråk etter engelsk for elever på barneskolene (se for eksempel Speitz et al 2007, Mordal et al 2012 og 2013. Evalueringene konkluderte med, etter begge forsøk, at elevene som oftest stortrives med å lære seg et 2. fremmedspråk allerede på barneskolen. Samtidig ble det avdekket et klart forskningsbehov når det gjelder avklaringer i forhold til fagets innhold, målsetninger og didaktisk-pedagogiske profil. Bidraget skal se på erfaringene man har samlet i Norge i og utenfor forsøkene. Dessuten skal det sees på andre lands erfaringer med en tidligere start på undervisningen i 2. fremmedspråk enn i Norge. Også norske erfaringer med tidlig undervisning i 2. fremmedspråk utenfor kommunale skoler, for eksempel ved Steinerskoler skal trekkes inn. På bakgrunn av slike erfaringer og rapportene etter forsøkene diskuteres først behov og utfordringer et slikt språkfag på barneskolen vil føre med seg. Deretter skisseres det hvilken struktur og hvilket innhold faget 2. fremmedspråk på barneskolen kan ha i framtiden.

  20. Revised results for geomechanical testing of MRIG-9 core for the potential SPR siting at the Richton Salt Dome.

    Energy Technology Data Exchange (ETDEWEB)

    Broome, Scott Thomas; Bauer, Stephen J.

    2010-02-01

    This report is a revision of SAND2009-0852. SAND2009-0852 was revised because it was discovered that a gage used in the original testing was mis-calibrated. Following the recalibration, all affected raw data were recalculated and re-presented. Most revised data is similar to, but slightly different than, the original data. Following the data re-analysis, none of the inferences or conclusions about the data or site relative to the SAND2009-0852 data have been changed. A laboratory testing program was developed to examine the mechanical behavior of salt from the Richton salt dome. The resulting information is intended for use in design and evaluation of a proposed Strategic Petroleum Reserve storage facility in that dome. Core obtained from the drill hole MRIG-9 was obtained from the Texas Bureau of Economic Geology. Mechanical properties testing included: (1) acoustic velocity wave measurements; (2) indirect tensile strength tests; (3) unconfined compressive strength tests; (4) ambient temperature quasi-static triaxial compression tests to evaluate dilational stress states at confining pressures of 725, 1450, 2175, and 2900 psi; and (5) confined triaxial creep experiments to evaluate the time-dependent behavior of the salt at axial stress differences of 4000 psi, 3500 psi, 3000 psi, 2175 psi and 2000 psi at 55 C and 4000 psi at 35 C, all at a constant confining pressure of 4000 psi. All comments, inferences, discussions of the Richton characterization and analysis are caveated by the small number of tests. Additional core and testing from a deeper well located at the proposed site is planned. The Richton rock salt is generally inhomogeneous as expressed by the density and velocity measurements with depth. In fact, we treated the salt as two populations, one clean and relatively pure (> 98% halite), the other salt with abundant (at times) anhydrite. The density has been related to the insoluble content. The limited mechanical testing completed has allowed us to

  1. Qualification and application of a surface plasmon resonance-based assay for monitoring potential HAHA responses induced after passive administration of a humanized anti Lewis-Y antibody.

    Science.gov (United States)

    Szolar, O H J; Stranner, S; Zinoecker, I; Mudde, G C; Himmler, G; Waxenecker, G; Nechansky, A

    2006-06-16

    A sensitive, surface plasmon resonance (SPR)-based assay monitoring potential human-anti-human antibody (HAHA) reactions against the monoclonal antibody (mAb) IGN311 is presented. The latter is a fully humanized Lewis-Y carbohydrate specific mAb that is currently tested in a passive immune therapy approach in a clinical phase I trial. For the SPR experiments a BIACORE 3000 analyzer was used. The ligand IGN311 was covalently coupled to the carboxy-methylated dextran matrix of a CM5 research grade chip (BIACORE). In the course of a fully nested experimental design, a four parameter logistic equation was identified as appropriate calibration model ranging from 0.3 microg/mL (lower limit of quantitation, LLOQ) to 200 microg/mL (upper limit of quantitation, ULOQ) using an anti-idiotypic mAb ('HAHA mimic') as calibrator. The bias ranged from -2.4% to 5.5% and the intermediate precision expressed as 95% CI revealed values from 5.6% to 8.3%. Specificity was evaluated using six human serum matrices from healthy donors spiked with calibrator at the limit of quantitation (LOQ) with >80% of values being recovered with less than 25% relative error. The qualified assay was applied to monitor potentially induced HAHA reactivity in 11 patients from a clinical phase I trial with passively administered IGN311. Of the 11 patients, one high HAHA responder and several low responders were identified. Protein-G depletion experiments with human serum samples revealed that the observed response is predominantly caused by IgG binding to the ligand. The characteristics of these HAHA responses were all of the so-called 'Type I' which is defined by a peak response around day 15 that decreases from this point steadily suggesting that some kind of tolerance is established. Therefore, this type of HAHA response is regarded as non critical for the patient's safety.

  2. Spræng boblen

    DEFF Research Database (Denmark)

    Hendricks, Vincent Fella

    Hvad har en dansk Justin Bieber look-a-like, Dovne Robert og frokostfrikadeller – eller mangel på samme – i landets børnehaver til fælles? De kan forstås og forklares med bobleteori. Bortset fra de af slagsen, der laves af sæbe eller findes i champagne, forbindes bobler typisk med situationer i...

  3. The SPR3 gene encodes a sporulation-specific homologue of the yeast CDC3/10/11/12 family of bud neck microfilaments and is regulated by ABFI.

    Science.gov (United States)

    Ozsarac, N; Bhattacharyya, M; Dawes, I W; Clancy, M J

    1995-10-16

    The SPR3 gene is selectively activated only during the sporulation phase of the Saccharomyces cerevisiae (Sc) life cycle. The predicted amino acid (aa) sequence has homology to microfilament proteins that are involved in cytokinesis and other proteins of unknown function. These include the products of Sc cell division cycle (CDC) genes involved in bud formation (Cdc3p, Cdc10p, Cdc11p and Cdc12p), Candida albicans proteins that accumulate in the hyphal phase (CaCdc3p and CaCdc10p), mouse brain-specific (H5p) and lymphocyte (Diff6p) proteins, Drosophila melanogaster (Dm) protein Pnutp (which is localized to the cleavage furrow of dividing cells), a Diff6p homologue (DmDiff6p), and the Sc septin protein (Sep1hp), a homologue of the 10-nm filament proteins of Sc. One strongly conserved region contains a potential ATP-GTP-binding domain. Primer extension analysis revealed six major transcription start points (tsp) beginning at -142 relative to the ATG start codon. The sequence immediately upstream from the tsp contains consensus binding sites for the HAP2/3/4 and ABFI transcription factors, a T-rich sequence and two putative novel elements for mid to late sporulation, termed SPR3 and PAL. Electrophoretic mobility shift assay (EMSA) and footprint analyses demonstrated that the ABFI protein binds to a region containing the putative ABFI site in vitro, and site-directed mutagenesis showed that the ABFI motif is essential for expression of SPR3 at the appropriate stage in sporulating cells.

  4. Principle and experiment of protein detection based on optical fiber sensing

    Science.gov (United States)

    Jiang, Qi; Xue, Mengwei; Liang, Pei; Zhang, Chengjia; Lin, Jianqiang; Ouyang, Jun

    2017-07-01

    A method for detecting protein molecules based on the tilted fiber Bragg grating (TFBG) surface plasma resonance (SPR) is proposed to achieve the quick online real-time detection of trace amount of proteins. The detection principles of the TFBG-SPR protein molecular probe are analyzed, and its feasibility is demonstrated. The intermediary material between the protein molecules and the golden layer outside of the fiber gratings is cysteamine hydrochloride. When the concentration of the cysteamine hydrochloride solution is 2 M, the shift of the TFBG resonance peak is 2.23 nm, illustrating that the cysteamine hydrochloride modifies the gold film successfully. IgG antigen solution is poured on the surface of the cysteamine hydrochloride modifying the gold-deposited TFBG. Finally, antigen-antibody hybridization experiment is carried out with a 10 mg/mL antibody solution, and after two hours of hybridization the resonance peak of the TFBG shifts 5.1 nm, which validates the feasibility and effectiveness of the TFBG-SPR protein molecular probe.

  5. 纳米金增强SPR检测产毒赭曲霉中PKS基因的特异性碱基%Detection for Polyketide Synthase Gene of Aspergillus ochraceus Based on Au Nanoparticles Enhancing SPR Biosensor

    Institute of Scientific and Technical Information of China (English)

    李迎; 林钊; 李蓉卓; 刘霞

    2012-01-01

    基于双通道表面等离子体子共振(surface plasmon resonance,SPR)传感器,分别采用直接法和金纳米粒子作为传感层的方法,通过检测赭曲霉毒素A(ochratoxin A,OTA)合成初期阶段表达的聚酮合成酶(polyketide synthase,PKS)基因的25个特异性碱基的寡核苷酸链,建立了一种高灵敏、间接检测OTA的新方法.同时考察了6-巯基己醇(6-mercapto-1-hexanol,MCH)作为封闭液对SPR响应信号的影响.结果表明,直接法的检测限为12.5 nmol/L,MCH的加入可使响应信号有所增强,使用金纳米粒子作为传感层的检测下限为0.25 nmol/L,与直接法相比较灵敏度提高了50倍,与以往使用金纳米粒子标记抗体或抗原相比,其作为传感层也能大大提高SPR检测灵敏度,且操作简单易行.%The surface plasmon resonance (SPR) method is established for indirect detection of the ochratoxin A. The 25-base oligonucleotide sequence of polyketide synthase (PKS) gene which is expressed during early stage of ochratoxin A synthesis, was detected respectively using direct assay and Au nanoparticles as sensing membrane assay based on the two channels surface plasmon resonance (SPR) biosensor. At the same time, influence of 6-mercapto-1-hexanol (MCH) blocking on SPR signal was also studied. As a result, the limit of detection (LOD) of the direct assay was 12.5 nmol/L. And the SPR signal was enhanced with 6-mercapto-l-hexanol (MCH) blocking. With Au nanoparticles as a sensing membrane, LOD was 0.25 nmol/L, sensitivity was 50 times higher than that of direct detection. SPR signal could be dramatically enhanced with Au nanoparticles as sensing membrane instead of as label, and the operation was simple.

  6. Research on Efficacy Evaluation Methods of Antibacterial Chinese Medicine Based on SPR Biosensor%基于SPR生物传感器的抗菌中药药效评价方法研究∗

    Institute of Scientific and Technical Information of China (English)

    张鹤林; 张国龙; 张康; 汪灿华

    2015-01-01

    目的:为抗菌中药药效进行实时检测,对药效评价给出定量分析。方法系采用真空热蒸镀法镀金属银膜制作出一种光纤SPR生物传感器,感测抗菌中药作用过程中抗原与抗体各个参数变化,为药效评价提供数据依据。该传感器的应用具有快速、客观、准确、高效的实时检测功能,为抗菌中药药效定量评价提供了依据。光纤SPR生物传感器的应用为抗菌中药药效评价给出了定量依据,有望最终用于指导临床准确应用抗菌中药。%Aiming at antibacterial effect of traditional Chinese medicine for real-time detection and analysis of quan-titative efficacy evaluation by the method of the vacuum thermal evaporation plating layer metal film. A fiber SPR sensor was produced,to make sense of the changes in antimicrobial antigen and antibody of each parameter in the process of Chinese medicine,and provide the data basis for efficacy evaluation. From results of real-time detection function,objective,accurate,fast and efficient application of the sensor,the basis was founded for the evaluation of the quantitative antibacterial effect of traditional Chinese medicine. Thus the application of fiber-optic SPR biosensor for antibacterial efficacy evaluation of traditional Chinese medicine has been set up on a quantitative basis. The aim for guiding the clinical application of traditional Chinese medicine and Chinese medicine accurate evalua-tion and selection is achieved by using the SPR sensor.

  7. Multiplexed magnetic nanoparticle-antibody conjugates (MNPs-ABS) based prognostic detection of ovarian cancer biomarkers, CA-125, β-2M and ApoA1 using fluorescence spectroscopy with comparison of surface plasmon resonance (SPR) analysis.

    Science.gov (United States)

    Pal, Manoj K; Rashid, Mohammad; Bisht, Manisha

    2015-11-15

    A multiplexed MNPs-Abs based fluorescence spectroscopic system in analysis of serum biomarkers; CA-125, β2-M and ApoA1 for the early detection of ovarian cancer was first time proposed. The lowest detection limits measured in multiplexed setup were 0.26 U/mL, 0.55 ng/mL and 7.7 ng/mL respectively for CA-125, β2-M and ApoA1. A comparative real sample analysis of healthy normal (Control), benign and ovarian cancer patients with SPR has also been done to validate the process. Moreover CA-125 detection only confirms 50-60% of early stage disease. This multiplexed system achieved sensitivity and specificity up to 94% and 98% respectively to distinguish early stage ovarian cancer patients from healthy individuals.

  8. High-resolution paleoclimatology of the Santa Barbara Basin during the Medieval Climate Anomaly and early Little Ice Age based on diatom and silicoflagellate assemblages in Kasten core SPR0901-02KC

    Science.gov (United States)

    Barron, John A.; Bukry, David B.; Hendy, Ingrid L.

    2015-01-01

    Diatom and silicoflagellate assemblages documented in a high-resolution time series spanning 800 to 1600 AD in varved sediment recovered in Kasten core SPR0901-02KC (34°16.845’ N, 120°02.332’ W, water depth 588 m) from the Santa Barbara Basin (SBB) reveal that SBB surface water conditions during the Medieval Climate Anomaly (MCA) and the early part of the Little Ice Age (LIA) were not extreme by modern standards, mostly falling within one standard deviation of mean conditions during the pre anthropogenic interval of 1748 to 1900. No clear differences between the character of MCA and the early LIA conditions are apparent. During intervals of extreme droughts identified by terrigenous proxy scanning XRF analyses, diatom and silicoflagellate proxies for coastal upwelling typically exceed one standard deviation above mean values for 1748-1900, supporting the hypothesis that droughts in southern California are associated with cooler (or La Niña-like) sea surface temperatures (SSTs). Increased percentages of diatoms transported downslope generally coincide with intervals of increased siliciclastic flux to the SBB identified by scanning XRF analyses. Diatom assemblages suggest only two intervals of the MCA (at ~897 to 922 and ~1151 to 1167) when proxy SSTs exceeded one standard deviation above mean values for 1748 to 1900. Conversely, silicoflagellates imply extreme warm water events only at ~830 to 860 (early MCA) and ~1360 to 1370 (early LIA) that are not supported by the diatom data. Silicoflagellates appear to be more suitable for characterizing average climate during the 5 to 11 year-long sample intervals studied in the SPR0901-02KC core than diatoms, probably because diatom relative abundances may be dominated by seasonal blooms of a particular year.

  9. Determination of binding capacity and adsorption enthalpy between Human Glutamate Receptor (GluR1) peptide fragments and kynurenic acid by surface plasmon resonance experiments.

    Science.gov (United States)

    Csapó, E; Majláth, Z; Juhász, Á; Roósz, B; Hetényi, A; Tóth, G K; Tajti, J; Vécsei, L; Dékány, I

    2014-11-01

    The interaction between kynurenic acid (KYNA) and two peptide fragments (ca. 30 residues) of Human Glutamate Receptor 201-300 (GluR1) using surface plasmon resonance (SPR) spectroscopy was investigated. Because of the medical interest in the neuroscience, GluR1 is one of the important subunits of the α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptors (AMPAR). AMPARs are ionotoropic glutamate receptors, which are mediating fast synaptic transmission and are crucial for plasticity in the brain. On the other hand, KYNA has been suggested to have neuroprotective activity and it has been considered for apply in therapy in certain neurobiological disorders. In this article the adsorption of the GluR1201-230 and GluR1231-259 peptides were studied on gold biosensor chip. The peptides were chemically bonded onto the gold surface via thiol group of L-cysteine resulted in the formation of peptide monolayer on the SPR chip surface. Because the GluR1231-259 peptide does not contain L-cysteine the Val256 was replaced by Cys256. The cross sectional area and the surface orientation of the studied peptides were determined by SPR and theoretical calculations (LOMETS) as well. The binding capability of KYNA on the peptide monolayer was studied in the concentration range of 0.1-5.0 mM using 150 mM NaCl ionic strength at pH 7.4 (±0.02) in phosphate buffer solutions. In order to determine the binding enthalpy the experiments were carried out between +10°C and +40°C. The heat of adsorption was calculated by using adsorption isotherms at different surface loading of KYNA on the SPR chip.

  10. Numerical simulations of lab-scale brine-water mixing experiments.

    Energy Technology Data Exchange (ETDEWEB)

    Khalil, Imane; Webb, Stephen Walter

    2006-10-01

    Laboratory-scale experiments simulating the injection of fresh water into brine in a Strategic Petroleum Reserve (SPR) cavern were performed at Sandia National Laboratories for various conditions of injection rate and small and large injection tube diameters. The computational fluid dynamic (CFD) code FLUENT was used to simulate these experiments to evaluate the predictive capability of FLUENT for brine-water mixing in an SPR cavern. The data-model comparisons show that FLUENT simulations predict the mixing plume depth reasonably well. Predictions of the near-wall brine concentrations compare very well with the experimental data. The simulated time for the mixing plume to reach the vessel wall was underpredicted for the small injection tubes but reasonable for the large injection tubes. The difference in the time to reach the wall is probably due to the three-dimensional nature of the mixing plume as it spreads out at the air-brine or oil-brine interface. The depth of the mixing plume as it spreads out along the interface was within a factor of 2 of the experimental data. The FLUENT simulation results predict the plume mixing accurately, especially the water concentration when the mixing plume reaches the wall. This parameter value is the most significant feature of the mixing process because it will determine the amount of enhanced leaching at the oil-brine interface.

  11. Surgical treatment of spasticity by selective posterior rhizotomy: 30 years experience.

    Science.gov (United States)

    Salame, Khalil; Ouaknine, Georges E R; Rochkind, Semion; Constantini, Shlomo; Razon, Nissim

    2003-08-01

    Spasticity is a common neurologic disorder with adverse effects on the patient's function. Conservative management is unsuccessful in a significant proportion of patients and neurosurgical intervention should be considered. The mainstay of surgical treatment of spasticity is selective posterior rhizotomy, i.e., section of sensory nerve roots of the cauda equina. To report our experience with selective posterior rhizotomy in the treatment of spasticity. We retrospectively reviewed our experience in 154 patients who underwent SPR during 30 years. The indication for surgery was spasticity that significantly hindered the patient's function or care and was resistant to conservative treatment. All patients were evaluated for spasticity in the lower and upper limbs, the presence or absence of painful spasms, and sphincter disturbances. The decision as to which roots to be sectioned, and to what extent, was based mainly on clinical muscle testing. Reduction of spasticity in the lower limbs was obtained in every case, with improvement in movements in 86% of cases. Painful spasms were alleviated in 80% of cases. Amelioration of neurogenic bladder was observed in 42%. A minority of the patients also showed improvement in speech and cognitive performance. There was no perioperative mortality or major complications. SPR is a safe and effective method for the treatment of spasticity with long-lasting beneficial effects. We suggest that this method be considered more frequently for patients with spasticity that interferes with their quality of life.

  12. A combined top-down/bottom-up approach to structuring multi-sensing zones on a thin film and the application to SPR sensors

    Science.gov (United States)

    Choi, Youngbo; Hyang Shin, So; Hong, Surin; Kim, Younghun

    2016-08-01

    The development of a thin film with well-defined metallic micro/nanostructures, diverse surface functionalities, and superior electronic/optical properties has been a great challenge to researchers seeking an efficient method for the detection of various analytes in chemical and biological sensing applications. Herein, we report a facile and effective approach to the fabrication of an ordered gold island pattern on a glass substrate with contrasted chemical functionalities, which can provide spatially separated sensing zones for multi-detection. In the proposed method, the combination between the micro/nano-imprint lithography and sequential self-assembly approaches exhibited synergistic effects that allowed well-defined structuring and easy surface functionalization in separated sensing zones. Via imprint lithography, the uniform gold islands/glass structure was successfully fabricated from a readily available gold-coated glass film. In addition, a sequential self-assembling strategy and specific chemical-substrate interactions, such as thiol-gold and silane-glass, enabled the surfaces of gold islands and exposed portions of the glass substrate with contrasting chemical functionalities—SH-functionalized gold islands and NH2-functionalized glass substrate. A proof-of-concept experiment for the multi-detection of heavy metal ions (Hg2+ and Cu2+) in an aqueous media was also successfully conducted using the dual-functionalized gold islands/glass structure and surface plasmon resonance measurements. The SH groups on the gold islands and the NH2 groups on the glass substrate functioned as spatially separated and selective receptors for Hg2+ and Cu2+ ions, respectively. Therefore, both the detection and quantification of Hg2+ and Cu2+ ions could be achieved using a single sensing substrate.

  13. Screening, identification of Rahnella sp.R3 producing cold-adapted lactase and its enzymatic properties%产低温乳糖酶菌株的筛选、鉴定及酶学性质的研究

    Institute of Scientific and Technical Information of China (English)

    沈莲莲; 杨瑞金; 华霄; 祁丹丹; 张文斌; 赵伟

    2013-01-01

    One cold-adapted lactase producing strain was isolated from Tiansan mount permafrost in the Xinjiang Uygur Autonomous Region.Based on its characteristics of morphology,cultivation,physiology and biochemistry and 16S rDNA sequence alignment,this strain was identified as Rahnella sp.and named Rahnella sp.R3.The optimal growth temperature of the strain and the optimal temperature for producing enzyme were both 15 ℃.Under the optimal fermentation conditions,the maximum enzyme activity was 5.92 U/mL.The lactase was intracellular enzyme.Research on its enzymatic properties showed that the lactase was maximally active at 25 ℃ and pH 7.0-8.0.And it had higher enzyme activity at low temperature,91% at 15 ℃ and 40% at 4 ℃,respectively.The enzyme activity was thermal-sensitive and was undetectable after 1 h incubated at 65 ℃.Some metal ions had inhibiting effects on enzyme activity.Ca2+ and Na + slightly reduced enzyme activity while heavy metal ions,particularly Cu2+,Al3+ and Zn2+ strongly inhibited enzyme activity.%从新疆天山冻土中分离获得到一株产低温乳糖酶的耐冷菌.根据形态学方法,生理生化试验、16S rDNA基因序列同源性比对及系统发育树构建对该菌株进行鉴定,结果显示该菌属于拉恩氏菌属(Rahnella sp.),为此命名为Rahnella sp.R3.该菌株最适生长温度和最适产酶温度均为15℃.在最适摇瓶条件下发酵液酶活为5.92 U/mL.该酶为胞内酶,在低温下具有较高活性,最适作用温度为25℃,15℃时的酶活为最高酶活的91%,4℃时的酶活为最高酶活的40%.该酶对热敏感,在65℃保温1h酶活力完全丧失.粗酶液的最适pH为7.5,在pH 6.5-8.0范围内显示良好的稳定性.一些金属离子对该乳糖酶有一定的抑制作用,其中,Na+、Ca2+对酶活性影响很小,Cu2+、Al3+、Zn2+对该酶的抑制作用明显.

  14. Avaliação de Sistema de Leitura Portátil (SLP para baixa visão desenvolvido no Brasil Evaluation of System Portable Reading (SPR for low vision developed in Brazil

    Directory of Open Access Journals (Sweden)

    Vagner Rogério dos Santos

    2009-10-01

    patients (40% presented AV 6,0M, 7 patients (23.3% presented AV 5M, 5 patients (16.7% presented 4M. The average of visual acuity before the utilization of SPR measured by LHNV-1 logMAR chart was 5,75M. After the use of LHNV-1 logMAR chart, 100% (one hundred percent of the patients reached the efficacy of AV J1. CONCLUSION: The prototype of SPR is an alternative resource in the social inclusion process of low vision patients with different levels of visual residue. It demonstrates psychological incentive, allows comfort, mobility and independence to those who need a more extended lecture and more distance of work.

  15. 基于SPR的双锥形光纤折射率传感特性研究%Research on Refractive Index Sensing Properties of Bioconical Optical Fiber Based on SPR

    Institute of Scientific and Technical Information of China (English)

    曹雨晨; 孙鹏

    2015-01-01

    The change of transmission optical power of optical fiber sensor in the liquid was studied and the liquid refractive index optical fiber sensor was proposed. The sensor used a 3 dB coupler to divide light. The wavelength of the light source ASE ranged from 1 530 nm to 1 580 nm.The fused taper fiber was used as sensing unit.The relationship between the light power of the transmission spectrum and the NaCl solution concentration was analyzed by using the optical fiber sensor.The results show that the higher the refractive index of the sensor external medium is,the bigger the transmission loss is. And coating on the outside of the cone can effectively improve the sensitivity of the sensor.%利用液体折射率与表面等离子共振技术( SPR),研究了光纤传感器在液体中透射光功率的变化,提出了一种液体折射率光纤传感器。该传感器采用3 dB耦合器进行分光,利用输出波长范围为1530~1580 nm的ASE作为光源,以熔融拉锥光纤作为传感单元。利用该光纤传感器,对NaCl溶液浓度与透射光功率之间关系的进行了实验研究。研究结果表明传感器外部介质折射率越高,光的传输损耗越大。而且在锥区外部镀膜,能够有效地提高传感器的灵敏度。

  16. 反射式光纤银膜SPR传感器的湿法构建及应用%Fabrication and Application of Reflective SPR Optical Fiber Biosensors Based on Silver Film

    Institute of Scientific and Technical Information of China (English)

    陈江萍; 苏荣欣; 齐崴; 黄仁亮; 王梦凡; 何志敏

    2015-01-01

    采用银镜反应湿法制备了反射式光纤表面等离子体共振(Surface plasmon resonance,SPR)传感器,并结合多巴胺自聚合功能对光纤表面银膜进行快速生物功能化修饰,实现了抗体抗原相互结合的在线监测.通过自行搭建SPR仪器,考察了传感区长度的影响,跟踪测定了传感器各个修饰步骤后SPR信号的变化.测试结果表明,该法制备的光纤SPR传感器具有很高的灵敏度.随着传感区长度的增加,基于SPR峰位的检测灵敏度随之增大,而基于SPR峰强的检测灵敏度无明显变化.当传感区长度为1.5 cm时,基于SPR峰位对蔗糖溶液折射率的检测灵敏度为4 166 nm/RIU,而基于SPR峰强的检测灵敏度为99%/RIU.进一步利用该传感器监测了IgG的连接过程、IgG抗体-抗原的相互结合过程,结果显示其可应用于生物传感检测领域.

  17. Revner, sprækker, skred

    DEFF Research Database (Denmark)

    Mønster, Louise

    2010-01-01

    The article analyses short stories by Helle Helle, Pia Juul and Naja Marie Aidt which address family and love relations, and it discusses the way in which they relate to presentations of new Danish literature. It is argued that these short stories are typical for the first decade of the 21th...... century in their focus on close relations, everyday life and contemporary society, whereas it seems more problematic to align them with the understanding of new Danish literature as a critique of the idea of cultural radicalism and the welfare state....

  18. SPR Hydrostatic Column Model Verification and Validation.

    Energy Technology Data Exchange (ETDEWEB)

    Bettin, Giorgia [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Lord, David [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Rudeen, David Keith [Gram, Inc. Albuquerque, NM (United States)

    2015-10-01

    A Hydrostatic Column Model (HCM) was developed to help differentiate between normal "tight" well behavior and small-leak behavior under nitrogen for testing the pressure integrity of crude oil storage wells at the U.S. Strategic Petroleum Reserve. This effort was motivated by steady, yet distinct, pressure behavior of a series of Big Hill caverns that have been placed under nitrogen for extended period of time. This report describes the HCM model, its functional requirements, the model structure and the verification and validation process. Different modes of operation are also described, which illustrate how the software can be used to model extended nitrogen monitoring and Mechanical Integrity Tests by predicting wellhead pressures along with nitrogen interface movements. Model verification has shown that the program runs correctly and it is implemented as intended. The cavern BH101 long term nitrogen test was used to validate the model which showed very good agreement with measured data. This supports the claim that the model is, in fact, capturing the relevant physical phenomena and can be used to make accurate predictions of both wellhead pressure and interface movements.

  19. Hatretorikk: Den intolerante språkbruken

    Directory of Open Access Journals (Sweden)

    Anne Birgitta Nilsen

    2014-03-01

    Full Text Available The manifesto of the Norwegian terrorist Anders Behring Breivik is based on the “Eurabia” conspiracy theory. This theory is a key starting point for hate speech amongst many right-wing extremists in Europe, but also has ramifications beyond these environments. In brief, proponents of the Eurabia theory claim that Muslims are occupying Europe and destroying Western culture, with the assistance of the EU and European governments. By contrast, members of Al-Qaeda and other extreme Islamists promote the conspiracy theory “the Crusade” in their hate speech directed against the West. Proponents of the latter theory argue that the West is leading a crusade to eradicate Islam and Muslims, a crusade that is similarly facilitated by their governments. This article presents analyses of texts written by right-wing extremists and Muslim extremists in an effort to shed light on how hate speech promulgates conspiracy theories in order to spread hatred and intolerance.The aim of the article is to contribute to a more thorough understanding of hate speech’s nature by applying rhetorical analysis. Rhetorical analysis is chosen because it offers a means of understanding the persuasive power of speech. It is thus a suitable tool to describe how hate speech works to convince and persuade. The concepts from rhetorical theory used in this article are ethos, logos and pathos. The concept of ethos is used to pinpoint factors that contributed to Osama bin Laden's impact, namely factors that lent credibility to his promotion of the conspiracy theory of the Crusade. In particular, Bin Laden projected common sense, good morals and good will towards his audience. He seemed to have coherent and relevant arguments; he appeared to possess moral credibility; and his use of language demonstrated that he wanted the best for his audience.The concept of pathos is used to define hate speech, since hate speech targets its audience's emotions. In hate speech it is the emotions that prevail, rather than reason. Sensational and dramatic claims are used to exploit existing feelings of anger, irritation and fear. The speech is aimed at those who may be persuaded of its negative content, and who may spread the message further. A distinct feature is its absence of logos: argumentation aimed at listeners' reason. To the extent logos is used in hate speech it is for the most part only apparent logos. The speech is often based on falsehoods, exaggerations, stereotypes, over-generalisations, and startling formulations. Hate speech therefore requires an uncritical audience – an audience that is either unable to see through the fallacies, or unwilling to do so because the arguments and conclusions fit well with their worldview. The overall aim of the article is to contribute to a more thorough understanding of hate speech’s nature and its role in disseminating conspiracy theories. However, through analyses of text examples from al-Qaeda’s leader, Osama bin Laden, and right-wing European extremists the article also contributes to explaining the terror attack in Oslo in July 2011 and the terror attack on September 11, 2001, in New York and other similar acts of terror.

  20. Revner, sprækker, skred

    DEFF Research Database (Denmark)

    Mønster, Louise

    2010-01-01

    The article analyses short stories by Helle Helle, Pia Juul and Naja Marie Aidt which address family and love relations, and it discusses the way in which they relate to presentations of new Danish literature. It is argued that these short stories are typical for the first decade of the 21th...

  1. The Annual Report of SPR Operation

    Institute of Scientific and Technical Information of China (English)

    1995-01-01

    4.2TheAnnualReportofSPROperationSunWeiwei*ChuShaochuIn1995,themaintasksofSPRweretheirradiationoffissionMo-Tctarget,isotopesan...

  2. Annual Report on SPR Operation in 2011

    Institute of Scientific and Technical Information of China (English)

    2011-01-01

    1 Information of operation Operation person: present chief operators are 11 persons, operators are 6 persons; new chief operators are 3 persons, new operator is 1 person; training chief operators are 3 persons, training operators are 1 persons;

  3. Quantifying cell binding kinetics mediated by surface-bound blood type B antigen to immobilized antibodies

    Institute of Scientific and Technical Information of China (English)

    LI BaoXia; CHEN Juan; LONG Mian

    2008-01-01

    Cell adhesion is crucial to many biological processes, such as inflammatory responses, tumor metastasis and thrombosis formation. Recently a commercial surface plasmon resonance (SPR)-based BIAcore biosensor has been extended to determine cell binding mediated by surface-bound biomolecular interactions. How such cell binding is quantitatively governed by kinetic rates and regulating factors, however, has been poorly understood. Here we developed a novel assay to determine the binding kinetics of surface-bound biomolecular interactions using a commercial BIAcore 3000 biosensor. Human red blood cells (RBCs) presenting blood group B antigen and CM5 chip bearing immobilized anti-B monoclonal antibody (mAb) were used to obtain the time courses of response unit, or sensorgrams, when flowing RBCs over the chip surface. A cellular kinetic model was proposed to correlate the sensorgrams with kinetic rates. Impacts of regulating factors, such as cell concentration,flow duration and rate, antibody-presenting level, as well as Ph value and osmotic pressure of suspending medium were tested systematically, which imparted the confidence that the approach can be applied to kinetic measurements of cell adhesion mediated by surface-bound biomolecular interactions.These results provided a new insight into quantifying cell binding using a commercial SPR-based BIAcore biosensor.

  4. Facile Tuning of SPR Absorption Band of Silver Nanoplates by Multi-stage Replacement or Growth%用多轮置换/生长法调节银纳米盘的SPR吸收峰

    Institute of Scientific and Technical Information of China (English)

    郭斌; 程建平; 罗江山; 吴卫东; 唐永建

    2009-01-01

    Single-crystalline triangular silver nanoplates synthesized by the dual-reduction method were used as template to replace HAuCl4 in a solution phase at room temperature. The in-plane dipolar surface plasmon resonance (SPR) absorption band of the Ag nanoplates locating initially at around 700 nm was gradually redshifted to 1100 nm via a multi-stage replacement manner after 9 stages of replacement, while the morphology of the template was evolved from solid plates to void triangular nanorings. The adding amount of HAuCl4 per stage could influence the average redshift value per stage, thus enabled a fine tuning of the in-plane dipolar band. And that band would slightly blueshift when HAuCl4 added in single stage reaction was below certain amount. When AgNO3 was reduced by ascorbic acid to deposit silver atoms onto surfaces of the nanoplates via a multi-stage reduction manner, the in-plane dipolar band gradually blueshifts to 650 nm after 8 stages of reduction, with the morphology of the template changing from triangle to larger and smoother round plate. These routes realized a facile stepwise tuning of the absorption band of Ag nanostructures within the visible-near-infrared region under mild conditions.%以双还原剂法制备的单晶三角形银纳米盘为模板,在室温溶液相与HAuCl4发生多轮置换反应,反应9轮后纳米盘的面内偶极表面等离子体共振峰从初始的700 nm逐步红移到1100 nm,形貌从实心盘状逐渐变为空心纳米环.改变每轮加入的HAuCl4的量即可精细调节面内偶极峰的红移步进量.在只进行一轮反应时,若加入的HAuCl4少于一定量,面内偶极峰会发生小幅度的蓝移.在银纳米盘模板溶液中用抗坏血酸还原AgNO3,银原子沉积到纳米盘上使其生长,若采用多轮还原生长的方法,生长8轮后纳米盘的面内偶极峰逐步蓝移至650 nm,形貌从三角形盘状逐渐变为更大更圆滑的圆盘状.以上方法实现了在温和反应条件下在可见

  5. [The experiment research on solution refractive index sensor based on tilted fiber Bragg grating].

    Science.gov (United States)

    Jiang, Qi; Lü, Dan-Dan; Yu, Ming-Hao; Kang, Li-Min; Ouyang, Jun

    2013-12-01

    The present paper analyzes the sensor's basic principle of the bare tilted fiber Bragg grating (TFBG) and surface plasmon resonance sensor (SPR) that deposited nanoscale gold-coating on the surface of the cladding. We simulated the transmission spectrums and some order cladding mode of TFBG in different concentration solutions by Integration and optical fiber grating software OptiGrating. So by the graphic observation and data analysis, a preliminary conclusion was got that in a certain sensing scope, the cladding modes of TFBG shift slightly to right with the increasing the solution refractive index(SRI),and the relation between resonance peak caused by the coupling of core mode and a certain cladding mode and the SRI was linear. Then the 45 nm thick gold coating was deposited on the surface of the TFBG cladding in a small-scale sputtering chamber KYKY SBC-12, and thermal field scanning electron microscopy presents that the effect of gold-coating was satisfactory to a certain extent in terms of microscopic level. The refractive index(RI) sensing experiments of different concentration solutions of NaCI, MgCI2, CaCI2 were carried out using bare and gold deposited TFBG. The RI sensing characteristics of both bare and gold deposited TFBGs respectively were studied by experiments. Meanwhile, it proved the conclusion that the cladding modes of TFBG drifted to right gradually when the SRI was increasing and the relations between resonance peak caused by the coupling of core mode and a certain cladding mode and the SRI were linear. And by quantitative analysis, we know that SPR sensor with the deposited namoscale gold layer on the surface of cladding enhanced the RI sensitivity dramatically by 2 to 500 nm RIU-1 which is 200 to 300 times larger than that of the bare tilted fiber Bragg grating approximately. The degrees of linear fittings of resonance peak caused by the coupling of core mode and a certain cladding mode and SRI of bare and gold-coating deposited SPR sensor

  6. G protein β1γ2 subunits purification and their interaction with adenylyl cyclase

    Institute of Scientific and Technical Information of China (English)

    CHEN; Julian; (陈巨莲); NI; Hanxiang; (倪汉祥); SUN; Jingrui; (孙京瑞); WENG; Gezhi

    2003-01-01

    A preliminary study on the interaction of G protein (guanine triphosphate binding pro- tein) β1γ2 subunits and their coupled components in cell signal transduction was conducted in vitro. The insect cell lines, Sf9 (Spodoptera frugiperda) and H5 (Trichoplusia ni) were used to express the recombinant protein Gβ1γ2. The cell membrane containing Gβ1γ2 was isolated through affinity chromatography column with Ni-NTA agarose by FPLC method, and the highly purified protein was obtained. The adenylyl cyclase 2 (AC2) activity assay showed that the purified Gβ1γ2 could significantly stimulate AC2 activity. The interaction of β1γ2 subunits of G protein with the cytoplasmic tail of various mammalian adenylyl cyclases was monitored by BIAcore technology using NTA sensor chip, which relies on the phenomenon of surface plasmon resonance (SPR). The experiments showed the direct binding of Gβ1γ2 to the cytoplasmic tail C2 domain of AC2. The specific binding domain of AC2 with Gβ1γ2 was the same as AC2 activity domain which was stimulated by β1γ2.

  7. Identification of protein phosphatase interacting proteins from normal and UVA-irradiated HaCaT cell lysates by surface plasmon resonance based binding technique using biotin-microcystin-LR as phosphatase capturing molecule.

    Science.gov (United States)

    Bécsi, Bálint; Dedinszki, Dóra; Gyémánt, Gyöngyi; Máthé, Csaba; Vasas, Gábor; Lontay, Beáta; Erdődi, Ferenc

    2014-09-05

    Identification of the interacting proteins of protein phosphatases is crucial to understand the cellular roles of these enzymes. Microcystin-LR (MC-LR), a potent inhibitor of protein phosphatase-1 (PP1), -2A (PP2A), PP4, PP5 and PP6, was biotinylated, immobilized to streptavidin-coupled sensorchip surface and used in surface plasmon resonance (SPR) based binding experiments to isolate phosphatase binding proteins. Biotin-MC-LR captured PP1 catalytic subunit (PP1c) stably and the biotin-MC-LR-PP1c complex was able to further interact with the regulatory subunit (MYPT1) of myosin phosphatase. Increased biotin-MC-LR coated sensorchip surface in the Surface Prep unit of Biacore 3000 captured PP1c, PP2Ac and their regulatory proteins including MYPT1, MYPT family TIMAP, inhibitor-2 as well as PP2A-A and -Bα-subunits from normal and UVA-irradiated HaCaT cell lysates as revealed by dot blot analysis of the recovered proteins. Biotin-MC-LR was used for the subcellular localization of protein phosphatases in HaCaT cells by identification of phosphatase-bound biotin-MC-LR with fluorescent streptavidin conjugates. Partial colocalization of the biotin-MC-LR signals with those obtained using anti-PP1c and anti-PP2Ac antibodies was apparent as judged by confocal microscopy. Our results imply that biotin-MC-LR is a suitable capture molecule in SPR for isolation of protein phosphatase interacting proteins from cell lysates in sufficient amounts for immunological detection.

  8. Detection and quantification of affinity ligand leaching and specific antibody fragment concentration within chromatographic fractions using surface plasmon resonance.

    Science.gov (United States)

    Thillaivinayagalingam, Pranavan; Newcombe, Anthony R; O'Donovan, Kieran; Francis, Richard; Keshavarz-Moore, Eli

    2007-12-01

    Rapid analyses of chromatographic steps within a biopharmaceutical manufacturing process are often desirable to evaluate column performance, provide mass balance data and to permit accurate calculations of yields and recoveries. Using SPR (surface plasmon resonance) biosensor (Biacore) technology, we have developed a sandwich immunoassay to quantify polyclonal anti-digoxin Fab fragments used for the production of the FDA (Food and Drug Administration)-approved biotherapeutic DigiFab. The results show that specific Fab may be quantified in all affinity process streams and accurate yield and mass balance data calculated. Control experiments using sheep Fab and Fc indicate that the assay is specific to DigiFab. The quantification of potential leached ligand within chromatographic fractions may also be technically challenging, particularly when low-molecular-mass ligands are covalently coupled with an affinity absorbent. Typical methods to assess ligand leakage such as DDMA (digoxin-dicarboxymethoxylamine; digoxin analogue) often involve the use of labelled ligands and relatively complex and labour-intensive analytical techniques. Using the same analytical methodologies, an assay to detect leached or eluted ligand off the column was developed. The results indicate minimal levels of leached ligand in all chromatographic fractions, with total levels of leached DDMA calculated to be 1.52 microg. This is less than 0.01% of the total amount of DDMA coupled with the laboratory-scale affinity column. The SPR methods described in the present study may be applicable for the rapid in-process analysis of specific polyclonal Fab fragments (within a polyclonal mixture) and to rapidly assess leakage of small molecule ligands covalently attached to chromatographic supports.

  9. Theoretical and Experimental Analysis of Adsorption in Surface-based Biosensors

    DEFF Research Database (Denmark)

    Hansen, Rasmus

    The present Ph.D. dissertation concerns the application of surface plasmon resonance (SPR) spectroscopy, which is a surface-based biosensor technology, for studies of adsorption dynamics. The thesis contains both experimental and theoretical work. In the theoretical part we develop the theory...... for convection, diffusion, and adsorption in surface-based biosensors in general. In particular, we study the transport dynamics in a model geometry of a Biacore SPR sensor. An approximate quasi-steady theory, which has been widely adopted in the SPR literature to capture convective and diffusive mass transport...... is critical for reliable use of the quasi-steady theory. Our theoretical results provide users of surface-based biosensors with a tool of correcting experimentally obtained adsorption rate constants, based on the quasisteady theory. Finally, the consequence of adsorption on all surfaces present in the flow...

  10. Experiment list: SRX080346 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available specific DNA binding protein that functions as an insulator, blocking enhancer activity. It has also been suggested to block the spr...eading of chromatin structure in certain instances. || a

  11. Experiment list: SRX080378 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available specific DNA binding protein that functions as an insulator, blocking enhancer activity. It has also been suggested to block the spr...eading of chromatin structure in certain instances. || a

  12. Lärares språkbruk i tvåspråkiga klassrum

    OpenAIRE

    Avery, Helen

    2011-01-01

    The article presents results of a study made in the context of introducing bilingual instruction in Swedish and Arabic. Classroom interaction was videotaped in grades one to four at two urban schools. Based on the video material, an inventory was made of how Arabic was used by the bilingual teachers, and how it related to the corresponding Swedish content. Simplified language use, code-switching and relations between use of Arabic and Swedish were analysed with respect to potential impact on ...

  13. Experiment list: SRX373275 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available SRX373275 ce10 Input control Input control Adult Young adult NA 20006523,13.5,7.8,3...59 GSM1259389: spr-5 G20 input rep1; Caenorhabditis elegans; ChIP-Seq source_name=whole young adult worm lys...ates || developmental stage=young adult || antibody=input || genotype=spr-5 || generation=G20 http://dbarchi

  14. Customer experience

    OpenAIRE

    Koperdáková, Zuzana

    2016-01-01

    Bachelor thesis deals with the theme of customer experience and terms related to this topic. The thesis consists of three parts. The first part explains the terms generally, as the experience or customer loyalty. The second part is dedicated to medotology used for Customer Experience Management. In the third part is described application of Customer Experience Management in practice, particularly in the context Touch Point Analyses in GE Money Bank.

  15. Psychology Experiments.

    Science.gov (United States)

    McGraw, Ken; Tew, Mark D.; Williams, John E.

    2001-01-01

    A goal of the PsychExperiments project was to reduce the financial burden on psychology departments for hardware/software used in their laboratories. In its third year, the PsychExperiments site now hosts 39 experiments. Over 200 classrooms worldwide have signed up as official site users and there have been nearly 10,000 data sessions conducted.…

  16. Psychology Experiments.

    Science.gov (United States)

    McGraw, Ken; Tew, Mark D.; Williams, John E.

    2001-01-01

    A goal of the PsychExperiments project was to reduce the financial burden on psychology departments for hardware/software used in their laboratories. In its third year, the PsychExperiments site now hosts 39 experiments. Over 200 classrooms worldwide have signed up as official site users and there have been nearly 10,000 data sessions conducted.…

  17. OPERA experiment

    CERN Document Server

    Komatsu, M

    2003-01-01

    OPERA is an accelerator experiment designed to explore Super- Kamiokande suggested nu//mu arrow left right nu//tau oscillation region in CNGS beam from CERN to LNGS. The key technique in OPERA is modern emulsion read out which applied to CHORUS and DONUT experiments. ECC technique which used in DONUT and OPERA has good modularity to enlarge apparatus for future Neutrino Factory experiments.

  18. Language Experience

    Science.gov (United States)

    Bugh, Marylou

    1978-01-01

    When a child uses his words and his ideas in learning to read, he also assists in the normal integration of his personality. Starting with a method of language experience developed by Sylvia Ashton-Warner, the author, a reading consultant, describes a language experience-reading program which utilizes the student's own curiosity and interests. (RK)

  19. Choice experiments

    Science.gov (United States)

    Thomas P Holmes; Wiktor L Adamawicz; Fredrik Carlsson

    2017-01-01

    There has been an explosion of interest during the past two decades in a class of nonmarket stated-preference valuation methods known as choice experiments. The overall objective of a choice experiment is to estimate economic values for characteristics (or attributes) of an environmental good that is the subject of policy analysis, where...

  20. The experience of an experience

    DEFF Research Database (Denmark)

    Rasmussen, Alexandra Marie

    2016-01-01

    This paper examines a popular phenomenon that allows us to see ourselves from various perspectives, and allows us to perpetuate moments enabling us to relive experiences in a different state of mind or at a different time. To examine the phenomenon of experiencing an experience hermeneutic...... phenomenological research was employed. The empirical data gathered in relation to this research emphasizes the experience of own experience. On a daily basis we create and are exposed to, what I have termed, the secondary experience (Exp2 ), which occurs when we review a video and/or pictures we have taken. I...... is a tool to create a particular mindset, in the developer, to generate a new way of thinking. This model is dynamic by nature and although it is not completed in every branch, it is complete in relation to experiencing your own experience. A company can apply the PoC to gain insight into primary...

  1. Collaborative experience

    DEFF Research Database (Denmark)

    Mortensen, Thomas Bøtker

    sample of firms, an establish way of measuring the outcome of product development and a new way of measuring experience. Where the previous research in this field primarily uses secondary databases, this research project collects primary data by an online questionnaire to the NPD manager from one......, that the largest effects from collaborative experience is from recent collaborative experience, since knowledge depreciates when it is not used. Methodologically contribution: The research project studies the dyad and aims at introducing, to this field of research, an established way of collecting data, a new...... of the new product development as a performance measure. Finally, where previous research primarily has used the number of collaborations as a measure of collaborative experience, this research includes the recency in the measure of collaborative experience. Results: Since data has not yet been collected...

  2. TRIO experiment

    Energy Technology Data Exchange (ETDEWEB)

    Clemmer, R.G.; Finn, P.A.; Malecha, R.F.; Misra, B.; Billone, M.C.; Bowers, D.L.; Fischer, A.K.; Greenwood, L.R.; Mattas, R.F.; Tam, S.W.

    1984-09-01

    The TRIO experiment is a test of in-situ tritium recovery and heat transfer performance of a miniaturized solid breeder blanket assembly. The assembly (capsule) was monitored for temperature and neutron flux profiles during irradiation and a sweep gas flowed through the capsule to an anaytical train wherein the amounts of tritium in its various chemical forms were determined. The capsule was designed to operate at different temperatures and sweep gas conditions. At the end of the experiment the amount of tritium retained in the solid was at a concentration of less than 0.1 wppM. More than 99.9% of tritium generated during the experiment was successfully recovered. The results of the experiment showed that the tritium inventories at the beginning and at the end of the experiment follow a relationship which appears to be characteristic of intragranular diffusion.

  3. Collaborative experience

    DEFF Research Database (Denmark)

    Mortensen, Thomas Bøtker

    Literature review: Collaborative experience has been shown to have a positive effect on the collaborative outcome in general (Anand & Khanna, 2000; Kale, Dyer & Singh, 2002). Furthermore, it has been linked to the ability to exploit the network of the firm for learning (Powell, Koput and Smith...... experience was largest the higher the hypothesized ambiguity. Theoretically contribution: This research project aims at contributing to existing literature by arguing, that collaborative experience is a moderating variable which moderates the effects on collaborative outcome from the level of complexity......, that the largest effects from collaborative experience is from recent collaborative experience, since knowledge depreciates when it is not used. Methodologically contribution: The research project studies the dyad and aims at introducing, to this field of research, an established way of collecting data, a new...

  4. 轻型车身自冲铆接头拉伸剪切实验的研究%Research of tensile shear experiments of self-piercing riveted joints for lightweight vehicle body

    Institute of Scientific and Technical Information of China (English)

    严柯科; 何晓聪; 邢保英; 张玉涛; 冯模盛

    2012-01-01

    Self-piercing riveting ( SPR) is a potential joining technology, especially for the light material (light alloy, composite and etc. ) joining. 2 mm-thick aluminum alloy 5052 specimens from serf-piercing riveting connections were studied, and the entireriveting process was analyzed. The self-piercing riveted structure was analyzed by applying the finite element analysis software AN-SYS. Tensile shear experiments of riveted specimens were conducted on AG - IS mechanics experimental machine. Experimental data of eight groups was analyzed and compared. Fracture modes of SPR joints were analyzed. Significance of vehicle weight reduction manufacturing on SPR joining technology was objectively evaluated%自冲铆接是一种很有潜力的连接工艺,尤其对于轻型材料(轻合金、复合材料等)的连接.文中对2 mm厚铝合金板5052的自冲铆连接试件进行了研究,分析了整个铆接过程;采用有限元分析软件ANSYS分析了自冲铆接结构;利用AG- IS力学实验机对铆接试件进行了拉伸剪切实验,通过8组实验数据分析对比,分析了铆接试件的断裂形式;客观地评价了自冲铆连接技术对汽车轻量化制造的重大意义.

  5. Mixture Experiments

    Energy Technology Data Exchange (ETDEWEB)

    Piepel, Gregory F.

    2007-12-01

    A mixture experiment involves combining two or more components in various proportions or amounts and then measuring one or more responses for the resulting end products. Other factors that affect the response(s), such as process variables and/or the total amount of the mixture, may also be studied in the experiment. A mixture experiment design specifies the combinations of mixture components and other experimental factors (if any) to be studied and the response variable(s) to be measured. Mixture experiment data analyses are then used to achieve the desired goals, which may include (i) understanding the effects of components and other factors on the response(s), (ii) identifying components and other factors with significant and nonsignificant effects on the response(s), (iii) developing models for predicting the response(s) as functions of the mixture components and any other factors, and (iv) developing end-products with desired values and uncertainties of the response(s). Given a mixture experiment problem, a practitioner must consider the possible approaches for designing the experiment and analyzing the data, and then select the approach best suited to the problem. Eight possible approaches include 1) component proportions, 2) mathematically independent variables, 3) slack variable, 4) mixture amount, 5) component amounts, 6) mixture process variable, 7) mixture of mixtures, and 8) multi-factor mixture. The article provides an overview of the mixture experiment designs, models, and data analyses for these approaches.

  6. Researching experiences

    DEFF Research Database (Denmark)

    Gjedde, Lisa; Ingemann, Bruno

    In the beginning was - not the word - but the experience. This phenomenological approach provides the basis for this book, which focuses on how a person-in-situation experiences and constructs meaning from a variety of cultural visual events. This book presents video-based processual methods...... for researching experiences in a variety of settings ranging from the museum, to news photography, and interactive media. The research led to the development of a set of methodological tools and approaches we term the reflexivity lab. The interaction in the experimental situation between the media and body...

  7. Antimatter Experiments

    CERN Multimedia

    2004-01-01

    Antimatter should behave in identical fashion to matter if a form of spacetime symmetry called CPT invariance holds. Two experiments at CERN near Geneva are testing this hypothesis using antihydrogen atoms

  8. Wanted: Experiments

    Science.gov (United States)

    McDaniel, Floyd D.

    1974-01-01

    Describes a project to produce a series of laboratory manuals and instructional materials in which nuclear experiments are presented for the undergraduate advanced laboratory. The manuals are being compiled in the areas of physics, chemistry, geology and environmental sciences. (BR)

  9. Pixel Experiments

    DEFF Research Database (Denmark)

    Petersen, Kjell Yngve; Søndergaard, Karin; Augustesen, Christina

    2015-01-01

    Pixel Experiments The term pixel is traditionally defined as any of the minute elements that together constitute a larger context or image. A pixel has its own form and is the smallest unit seen within a larger structure. In working with the potentials of LED technology in architectural lighting...... design it became relevant to investigate the use of LEDs as the physical equivalent of a pixel as a design approach. In this book our interest has been in identifying how the qualities of LEDs can be used in lighting applications. With experiences in the planning and implementation of architectural...... elucidate and exemplify already well-known problems in relation to the experience of vertical and horizontal lighting. Pixel Experiments exist as a synergy between speculative test setups and lighting design in practice. This book is one of four books that is published in connection with the research...

  10. Authoring experience

    DEFF Research Database (Denmark)

    Knox, Jeanette Bresson Ladegaard; Svendsen, Mette Nordahl

    2015-01-01

    This article examines the storytelling aspect inphilosophizing with rehabilitating cancer patients in small Socratic dialogue groups (SDG). Recounting an experienceto illustrate a philosophical question chosen by the participantsis the traditional point of departure for the dialogicalexchange. Ho...... an experiencethrough a collaborative effort, most participants hadtheir initial experience existentially refined and the chosenconcept of which the experience served as an illustrationtransformed into a moral compass to be used in self-orientationpost cancer....

  11. The Experiment

    Science.gov (United States)

    Mariana Nicoara, Floare

    2016-04-01

    My name is Nicoara Floarea and I am teacher at Secondary School Calatele and I teach students from preparatory class and the second grade . They are six-eight years old. In my activity, for introducing scientific concepts to my students, I use various and active methods or traditional methods including experiments. The experiment stimulates students' curiosity, their creativity, the understanding and knowledge taught accessibility. I propose you two such experiments: The life cycle of the plants (long-term experiment, with rigorous observation time):We use beans, wheat or other; They are grown in pots and on the cotton soaked with water,keeping under students' observation protecting them ( just soak them regularly) and we waiting the plants rise. For discussions and comments of plant embryo development we use the plants which rose on the cotton soaked with water plants at the end of the first week. Last school year we had in the pot climbing beans which in May made pods. They were not too great but our experiment was a success. The students could deduce that there will develop those big beans which after drying will be planted again. The influence of light on plants (average duration experiment with the necessary observation time): We use two pots in which plants are of the same type (two geraniums), one of them is situated so as to get direct sunlight and other plant we put in a closed box. Although we wet both plants after a week we see that the plant that benefited from sunlight has turned strain in direct sunlight, developing normally in return the plant out of the box I have yellowed leaves, photosynthesis does not She has occurred . Students will understand the vital role of the Sun in plants' life, both in the classroom and in nature. The experiment is a method of teaching students extremely pleasant, with a remarkable percentage of acquiring more knowledge.

  12. Stability and Load Bearing Capacity of a Bars with Built up Cross Section and Elastic Supports / Badania Stateczności I Nosności Prętów Złożonych Z Podporami Sprężystymi

    Science.gov (United States)

    Krajewski, Marcin

    2015-03-01

    The present paper is devoted to the numerical analysis and experimental tests of compressed bars with built-up cross section which are commonly used as a top chord of the roof trusses. The significant impact on carrying capacity for that kind of elements in case of out-of-plane buckling is appropriate choice of battens which are used to provide interaction between separate members. Linear buckling analysis results and nonlinear static analysis results, with material and geometrical nonlinearity, are presented for the bar with built-up cross section which was used as the top chord of the truss made in reality. Diagonals and verticals which are supports for the top chord between marginal joints were replaced by the elastic supports. The threshold stiffness (minimum stiffness) for the intermediate elastic supports which ensures maximum buckling load was appointed for the beam and shell model of the structure. The magnitude of limit load depended on length of the battens was calculated for models with initial geometric imperfections. The experimental tests results for the axially compressed bars with builtup cross section and elastic support are presented. Niniejsza praca poświecona jest analizom numerycznym i badaniom doświadczalnym ściskanych prętów złożonych, które są często stosowane, jako pasy górne kratownic dachowych. Istotny wpływ na nośność tego typu elementów, przy założeniu wyboczenia z płaszczyzny układu, ma odpowiedni dobór przewiązek zapewniający współpracę poszczególnych gałęzi. W pracy przedstawiono rezultaty liniowych analiz stateczności oraz fizycznie i geometrycznie nieliniowych analiz statycznych dla pręta złożonego, z którego zbudowany jest pas górny kratownicy wykonanej w rzeczywistości. Słupki i krzyżulce podpierające pas między węzłami skrajnymi zastąpiono podporami sprężystymi. Wyznaczono graniczną (minimalna) sztywność sprężystych podpór pośrednich zapewniającą maksymalną wartość obci

  13. Extending Experiences

    DEFF Research Database (Denmark)

    A computer game's player is experiencing not only the game as a designer-made artefact, but also a multitude of social and cultural practices and contexts of both computer game play and everyday life. As a truly multidisciplinary anthology, Extending Experiences sheds new light on the mesh...... of possibilities and influences the player engages with. Part one, Experiential Structures of Play, considers some of the key concepts commonly used to address the experience of a computer game player. The second part, Bordering Play, discusses conceptual and practical overlaps of games and everyday life...

  14. Poetic Experience

    Directory of Open Access Journals (Sweden)

    Shahab Yar Khan

    2014-01-01

    Full Text Available Nature of poetic experience is hereby redefined. The present article initially deals with the perennial nature of true poetic experience and its essential relevance to the world. It attempts to elaborate the process through which a poet is uplifted in a creative moment beyond terrestrial boundaries and is aligned with the ‘state of Perfection'. The role of successive generations of audiences in rediscovering the meaning of a poetic image is defined as life principle of all great poetry. Shakespeare is discussed as the ultimate example of this principle since his popularity remains an irreversible phenomenon

  15. Media experiences

    DEFF Research Database (Denmark)

    Buhl, Mie

    2010-01-01

    The paper discusses mediated experiences from the perspective of the visual modality in combination with the multimodal interaction. ICT-studies has a rapid influx of new words and concepts. Digital technology led to a need to describe the convergence of images, text and sound has taken various...

  16. Aesthetic experience.

    Science.gov (United States)

    Sirois, François J

    2008-02-01

    An initial clinical question, 'Why does an analysand talk about his/her relationship with an aesthetic object?' opens an investigation into the nature of aesthetic experience. Three principal aspects of the psychoanalytic approach are presented: sublimation, a Freudian concept concerning the vicissitudes of the drives; reparation, a Kleinian concept linked to depressive anxiety; and transformation, a concept of object-relations theory about primitive ego-states. The article discusses the psychic function of aesthetic feelings in mastering anxiety as related to ego, id and superego. The transformation of the experience of passivity is a common link underlying these aspects. Such transformation relies on tolerating ambiguous and contrary feelings within the self, fostered by contact with an aesthetic object. This balance can, however, be upset: two excessive forms of aesthetic experience ensue, namely, fascination and bewitchment. The first belongs to the experience of awe; the second can lead to claustrophobic anxiety. The initial clinical question requires an elaboration of aesthetic transference, a variant of the narcissistic transference, whereby the analysand invites the analyst to share his/her internal state as a common unspoken object.

  17. Dirac experiment

    CERN Document Server

    Gómez, F; Afanasev, L; Benayoun, M; Brekhovskikh, V; Caragheorgheopol, G; Cechák, T; Chiba, M; Constantinescu, S; Doudarev, A; Dreossi, D; Drijard, Daniel; Ferro-Luzzi, M; Gallas, M V; Gerndt, J; Giacomich, R; Gianotti, P; Goldin, D; Gorin, A; Gortchakov, O; Guaraldo, C; Hansroul, M; Hosek, R; Iliescu, M; Jabitski, M; Kalinina, N; Karpoukhine, V; Kluson, J; Kobayshi, M; Kokkas, P; Komarov, V; Koulikov, A; Kouptsov, A; Krouglov, V; Krouglova, L; Kuroda, K I; Lanaro, A; Lapshine, B; Lednicky, R; Leruste, P; Levisandri, P; López-Aguera, A; Lucherini, V; Mäki, T; Manuilov, I; Montanet, L; Narjoux, J L; Nemenov, L; Nikitin, M; Nunez Pardo, T; Okada, K; Olchevskii, V; Pazos, A; Pentia, M; Penzo, Aldo L; Perreau, J M; Petrascu, C; Pló, M; Ponta, T; Pop, D; Riazantsev, A; Rodríguez, J M; Rodriguez Fernandez, A; Rykaline, V; Santamarina, C; Saborido, J; Schacher, J; Sidorov, A; Smolik, J; Takeutchi, F; Tarasov, A; Tauscher, L; Tobar, M J; Trusov, S; Vasquez, P; Vlachos, S; Yazkov, V; Yoshimura, Y; Zrelov, P

    2001-01-01

    The main objective of DIRAC experiment is the measurement of the lifetime tau of the exotic hadronic atom consisting of pi /sup +/ and pi /sup -/ mesons. The lifetime of this atom is determined by the decay mode pi /sup +/ pi /sup -/ to pi /sup 0/ pi /sup 0/ due to the strong interaction. Through the precise relationship between the lifetime and the S-wave pion-pion scattering length difference a/sub 0/-a/sub 2/ for isospin 0 and 2 (respectively), a measurement of tau with an accuracy of 10% will allow a determination of a/sub 0/-a/sub 2/at a 5% precision level. Pion-pion scattering lengths have been calculated in the framework of chiral perturbation theory with an accuracy below 5%. In this way DIRAC experiment will provide a crucial test of the chiral symmetry breaking scheme in QCD effective theories at low energies. (19 refs).

  18. Crucible Experiments

    Science.gov (United States)

    Jiang, Min; Wang, Xin-Hua; Pak, Jong-Jin

    2014-08-01

    ZrO2 crucible experiments were carried out in the laboratory to study the formation of low-melting-point inclusions in steel, during which aluminum deoxidization and a high-basicity calcium aluminate refining slag (CaO/SiO2: 6 to 8, Al2O3 40 to 45 pct) were used. Four experiments were done with different slag/steel reaction times (30, 60, 90, and 180 minutes). It was found that inclusions were mainly composed of CaO-Al2O3-ZrO2 with very limited SiO2, in spherical morphology and with sizes mainly less than 5 μm. They can be classified into two types according to the ZrO2 content. The first type contained much lower ZrO2, whereas a much higher level of ZrO2 was detected in the other type. An evolution of inclusions with the reaction time was observed and studied. The obtained results indicated that chemical compositions of inclusions were widely scattered in the CaO-Al2O3-ZrO2 phase diagram after 30 min reaction. However, the composition of inclusions became much more uniform and concentrated in low-melting-temperature regions at 60, 90, and 180 minutes, which would be favorable to prevent nozzle clogging and fatigue problems. Compared with the authors' previous results obtained in MgO crucible experiments, it was found that low-melting-temperature inclusions can be targeted in shorter time in ZrO2 crucible experiments, without any degradation of cleanliness.

  19. Calculation of the radionuclides in PWR spent fuel samples for SFR experiment planning.

    Energy Technology Data Exchange (ETDEWEB)

    Naegeli, Robert Earl

    2004-06-01

    This report documents the calculation of radionuclide content in the pressurized water reactor (PWR) spent fuel samples planned for use in the Spent Fuel Ratio (SPR) Experiments at Sandia National Laboratories, Albuquerque, New Mexico (SNL) to aid in experiment planning. The calculation methods using the ORIGEN2 and ORIGEN-ARP computer codes and the input modeling of the planned PWR spent fuel from the H. B. Robinson and the Surry nuclear power plants are discussed. The safety hazards for the calculated nuclide inventories in the spent fuel samples are characterized by the potential airborne dose and by the portion of the nuclear facility hazard category 2 and 3 thresholds that the experiment samples would present. In addition, the gamma ray photon energy source for the nuclide inventories is tabulated to facilitate subsequent calculation of the direct and shielded dose rates expected from the samples. The relative hazards of the high burnup 72 gigawatt-day per metric ton of uranium (GWd/MTU) spent fuel from H. B. Robinson and the medium burnup 36 GWd/MTU spent fuel from Surry are compared against a parametric calculation of various fuel burnups to assess the potential for higher hazard PWR fuel samples.

  20. Strategies for the detection of food pathogens and contaminants

    DEFF Research Database (Denmark)

    Hearty, Stephen; Leonard, Paul; Sheehan, Alfredo Darmanin

    that molecular contaminants such as microbial toxins and drug/pesticide residues translate well onto Biacore-based assay formats. However, larger and more complex entities such as spores and whole bacterial cells represent an altogether more difficult challenge. Here, we present an overview of our experiences......We routinely use Biacore for affinity ranking and kinetic characterisation of diverse panels of hybridoma-derived and recombinant antibodies against a wide range of different clinically relevant antigens for diagnostic applications. Generally the analytes of interest are haptens or defined protein...

  1. Strategies for the detection of food pathogens and contaminants

    DEFF Research Database (Denmark)

    Hearty, Stephen; Leonard, Paul; Sheehan, Alfredo Darmanin

    2006-01-01

    that molecular contaminants such as microbial toxins and drug/pesticide residues translate well onto Biacore-based assay formats. However, larger and more complex entities such as spores and whole bacterial cells represent an altogether more difficult challenge. Here, we present an overview of our experiences......We routinely use Biacore for affinity ranking and kinetic characterisation of diverse panels of hybridoma-derived and recombinant antibodies against a wide range of different clinically relevant antigens for diagnostic applications. Generally the analytes of interest are haptens or defined protein...

  2. Experiment summary

    CERN Document Server

    Rossi, Andrea

    2015-01-01

    The measurement of the production of particles coming from hard scattering processes covers a fundamental role in the characterization of the system formed in heavy-ion collisions, allowing to probe the microscopic processes underlying the interaction of high energy partons with the medium. An impressive amount of measurements related to jet, quarkonia, open heavy flavor, and electroweak signal production in nucleus-nucleus as well as p(d)-nucleus collisions was delivered by experiments at RHIC and LHC in past years. In these proceedings, the main experimental results presented during the Hard Probes conference are summarized.

  3. QUBIC Experiment

    CERN Document Server

    Stolpovskiy, Mikhail

    2016-01-01

    QUBIC is a ground-based experiment, currently under construction, that uses the novel bolometric interferometry technology. It is dedicated to measure the primordial B-modes of CMB. As a bolometric interferometer, QUBIC has high sensitivity and good systematics control. Dust contamination is controlled by operating with two bands -- 150 and 220 GHz. There are two possible sites for QUBIC: either Concordia station in Antarctic or in the Argentinian Puna desert. It is planned to see the first light in 2018-2019.

  4. Media experiences

    DEFF Research Database (Denmark)

    Buhl, Mie

    2010-01-01

    be interpreted as legible signs, i.e. their meaning can be translated into written or oral language (Kress and van Leuwen 1996; Kress 2003). However, the embodied sense-based qualities involved in images and visualisations cannot be fully comprehended from a discursive perspective. Images and visualisations draw...... on their particular iconic systems of meaning perceived through form, lines, contrasts, colours, structures textures, rhythm and composite qualities with embedded interdependent codes and culturally conditioned potential for construction of knowledge and meaning. Mediated experience adds new perspectives...

  5. Thomson Experiment

    CERN Multimedia

    This experiment, conducted by JJ Thomson in 1897, established the existence of the electron. Thomson won the Nobel physics prize for this work in 1906. A beam of electrons crosses the chamber emitting blue light. Adding an electric field (E) or a magnetic field (B) exerts a force on the moving electrons.Use switch E to turn on the electric field in the chamber. Then, by turning knob B, you can increase the current in the coils, generating a magnetic field. By balancing the electric and magnetic fields, Thomson was able to keep the electron beam level and deduce the ratio of the electron's charge to its mass.

  6. Investigation of oil injection into brine for the strategic petroleum reserve : hydrodynamics experiments with simulant liquids.

    Energy Technology Data Exchange (ETDEWEB)

    Castaneda, Jaime N.; Shollenberger, Kim Ann (California Polytechnic State University, San Luis Obispo, CA); Torczynski, John Robert; Cote, Raymond O.; Barney, Jeremy; O' Hern, Timothy John

    2003-10-01

    An experimental program is being conducted to study a proposed approach for oil reintroduction in the Strategic Petroleum Reserve (SPR). The goal is to assess whether useful oil is rendered unusable through formation of a stable oil-brine emulsion during reintroduction of degassed oil into the brine layer in storage caverns. This report documents the first stage of the program, in which simulant liquids are used to characterize the buoyant plume that is produced when a jet of crude oil is injected downward from a tube into brine. The experiment consists of a large transparent vessel that is a scale model of the proposed oil injection process at the SPR. An oil layer is floated on top of a brine layer. Silicon oil (Dow Corning 200{reg_sign} Fluid, 5 cSt) is used as the simulant for crude oil to allow visualization of the flow and to avoid flammability and related concerns. Sodium nitrate solution is used as the simulant for brine because it is not corrosive and it can match the density ratio between brine and crude oil. The oil is injected downward through a tube into the brine at a prescribed depth below the oil-brine interface. Flow rates are determined by scaling to match the ratio of buoyancy to momentum between the experiment and the SPR. Initially, the momentum of the flow produces a downward jet of oil below the tube end. Subsequently, the oil breaks up into droplets due to shear forces, buoyancy dominates the flow, and a plume of oil droplets rises to the interface. The interface is deflected upward by the impinging oil-brine plume. Two different diameter injection tubes were used (1/2-inch and 1-inch OD) to vary the scaling. Use of the 1-inch injection tube also assured that turbulent pipe flow was achieved, which was questionable for lower flow rates in the 1/2-inch tube. In addition, a 1/2-inch J-tube was used to direct the buoyant jet upwards rather than downwards to determine whether flow redirection could substantially reduce the oil-plume size and the

  7. Medical microbiology training needs and trainee experience.

    Science.gov (United States)

    Seale, Josephine; Elamin, Wael; Millar, Michael

    2014-02-01

    Training in microbiology is continuing to evolve. Standardisation of this process has, in part, been achieved through the development of a training curriculum by the Royal College of Pathologists (RCPath). A substantial proportion of microbiology training occurs through telephone consultations. To ascertain the content of these interactions and the extent to which the necessary skills outlined by the curriculum are attainable via these consultations. Records of telephone consultations made by microbiology registrars (SpR) on the Laboratory Information Management System (LIMS) over a 6 month period were analysed with regard to who initiated contact and the type of advice provided. An average of 426 SpR entries per month were made on the LIMS following telephone consultations. These consultations were predominantly initiated by fellow clinicians as opposed to the SpR. The majority (79%) of advice entailed guidance as to the use of antimicrobials which resulted in an alteration of the current regimen in 54% of cases. This study represents the first attempt to quantify the telephone consultations of microbiology trainees. It is concluded that although such interactions provide a means of attaining some of the competencies outlined by the RCPath curriculum, the bias towards antimicrobial advice reflects a discrepancy between the needs of the service users and the broad skill set advocated by the current microbiology training programme. Future modifications will need to take this into account to ensure both the training of SpRs and the microbiology service is fit for purpose.

  8. Experimenting with a design experiment

    Directory of Open Access Journals (Sweden)

    Bakker, Judith

    2012-12-01

    Full Text Available The design experiment is an experimental research method that aims to help design and further develop new (policy instruments. For the development of a set of guidelines for the facilitation of citizens’ initiatives by local governments, we are experimenting with this method. It offers good opportunities for modeling interventions by testing their instrumental validity –the usefulness for the intended practical purposes. At the same time design experiments are also useful for evaluating the empirical validity of theoretical arguments and the further development of these arguments in the light of empirical evidence (by using e.g. the technique of pattern matching. We describe how we have applied this methodology in two cases and discuss our research approach. We encountered some unexpected difficulties, especially in the cooperation with professionals and citizens. These difficulties complicate the valid attribution of causal effects to the use of the new instrument. However, our preliminary conclusion is that design experiments are useful in our field of study

    El experimento de diseño es un método de investigación experimental que tiene como objetivo diseñar y desarrollar posteriormente nuevas herramientas (políticas. En este artículo experimentamos con este método para desarrollar un conjunto de directrices que permitan a los gobiernos locales facilitar las iniciativas ciudadanas. El método ofrece la oportunidad de modelar las intervenciones poniendo a prueba su validez instrumental (su utilidad para el fin práctico que se proponen. Al mismo tiempo, los experimentos de diseño son útiles también para evaluar la validez empírica de las discusiones teóricas y el posterior desarrollo de esas discusiones a la luz de la evidencia empírica (usando, por ejemplo, técnicas de concordancia de patrones. En este trabajo describimos cómo hemos aplicado este método a dos casos y discutimos nuestro enfoque de

  9. Rutherford Experiment

    CERN Multimedia

    This experiment, carried out by Ernest Rutherford in 1910, revolutionised understanding of the structure of matter, showing that almost all the mass of an atom is concentrated in a very small, positively charged nucleus. Alpha particles emitted at bombard a thin gold foil. A detector records the number of alpha particles crossing the foil per second. The number is displayed on the counter and updated every minute. Alpha particles are helium nuclei, they consist of 2 protons and 2 neutrons. Rotate the central knob to change the angle between the foil and the detector. The number of alpha particles detected depends on the angle. Most of the alpha particles travel straight through the foil because the gold atoms are mainly empty space. However some hit the atomic nucleus and are deflected.

  10. Management Experiences

    Directory of Open Access Journals (Sweden)

    Gheorghe Popovici

    2016-12-01

    Full Text Available The paper presents the results of the survey about the changes in modern management, identified from the experience of Romanian managers. By this online study one presents both the obstacles encountered and the recommendations for such a type of management that the present and future mangers must take into account. What motivated the respondent Romanian managers most to open their own business is the independence it offered them. They work in the field they have liked since they were young. The second reason was the perspective to have an additional income from the business development. The third argument in favour of opening a business is the possibility to assure the balance between personal life and career.

  11. 杀菌/渗透增强蛋白功能区抗菌肽与内毒素亲和性的SPR技术检测%SPR detection of affinity of antibacterial peptides in bactericidal/permeability-increasing protein domain for endotoxin

    Institute of Scientific and Technical Information of China (English)

    李晶琴; 孔庆利; 樊峥

    2010-01-01

    目的:研究杀菌/渗透增强蛋白(BPI)不同功能区抗菌肽与内毒素(LPS)结合的能力.方法:设计、合成来自杀菌/渗透增强蛋白N端不同功能区的3段抗菌肽,分别为BPI22-36、BPI85-99和BPI147-161,将其固定于CM5蛋白质芯片上,使用表面等离子共振(SPR)仪检测3种抗菌肽与LPS和类脂A(Lipid A)相互作用的情况.结果:3种抗菌肽中,BPI147-161与LPS和Lipid A结合的能力最强,BPI85-99次之,但BPI22-36与其不结合;BPI147-161与Lipid A的亲和常数KA为1.12×106L/mol,相比,多黏菌素B(PMB)的亲和常数KA为5.58 ×106L/mol.结论:来自杀菌/渗透增强蛋白的抗菌肽BPI147-161能有效地中和内毒素,这可能为败血症休克的治疗提供一种新的策略.

  12. Multifunctional magneto-plasmonic nanotransducers for advanced theranostics: synthesis, modeling and experiment

    Science.gov (United States)

    Masoumi, Masoud; Wang, Ya; Liu, Mingzhao; Tewolde, Mahder; Longtin, Jon

    2015-04-01

    In this work, nano-transducers with a superparamagnetic iron oxide (SPIO) core have been synthesized by preparation of precursor gold nanoseeds loaded on SPIO-embedded silica to form a gold nanoshell. The goal is for such nanotansducers to be used in theranostics to detect brain tumors by using MRI imaging and then assist in their treatment by using photothermal ablation. The iron oxide core provides for the use of a magnetic-field to guide the particles to the target (tumor) site. The gold nanoshell can be then readily heated using incident light and/or an alternating magneticfield. After synthesis of nano-transducer samples, Transmission Electron Microscopy was employed to analyze the formation of each layer. Then UV spectroscopy experiments were conducted to examine the light absorbance of the synthesized samples. The UV-visible absorption spectra shows a clear surface plasmon resonance (SPR) band around 530 nm, verifying the presence of gold coating nanoshells. Finally photothermal experiments using a high-power laser beam with a wavelength of 527 nm were performed to heat the samples. It was found that the temperature reaches 45° C in 12 minutes.

  13. Strategies for the detection of food pathogens and contaminants

    DEFF Research Database (Denmark)

    Hearty, Stephen; Leonard, Paul; Sheehan, Alfredo Darmanin;

    that molecular contaminants such as microbial toxins and drug/pesticide residues translate well onto Biacore-based assay formats. However, larger and more complex entities such as spores and whole bacterial cells represent an altogether more difficult challenge. Here, we present an overview of our experiences...

  14. Experiment list: SRX373269 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available SRX373269 ce10 TFs and others cec-3 Adult Young adult NA 6517898,1.8,30.5,65 GSM125...9383: spr-5 G20 rep1; Caenorhabditis elegans; ChIP-Seq source_name=whole young adult worm lysates || developmental stage=you

  15. Experiment list: SRX373271 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available SRX373271 ce10 TFs and others cec-3 Adult Young adult NA 18870642,40.6,40.4,563 GSM...1259385: spr-5 G20 rep2 #2; Caenorhabditis elegans; ChIP-Seq source_name=whole young adult worm lysates || developmental stage=you

  16. Experiment list: SRX373274 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available SRX373274 ce10 Input control Input control Adult Young adult NA 30925633,64.9,11.5,...0 GSM1259388: spr-5 G10 input; Caenorhabditis elegans; ChIP-Seq source_name=whole young adult worm lysates || developmental stage=you

  17. Experiment list: SRX102978 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available tor, blocking enhancer activity. It has also been suggested to block the spreadin... specific DNA binding protein that functions as an insulator, blocking enhancer activity. It has also been suggested to block the spr...eading of chromatin structure in certain instances. || a

  18. Experiment list: SRX102979 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available sulator, blocking enhancer activity. It has also been suggested to block the spread... specific DNA binding protein that functions as an insulator, blocking enhancer activity. It has also been suggested to block the spr...eading of chromatin structure in certain instances. || a

  19. Experiment list: SRX102977 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available sulator, blocking enhancer activity. It has also been suggested to block the spread... specific DNA binding protein that functions as an insulator, blocking enhancer activity. It has also been suggested to block the spr...eading of chromatin structure in certain instances. || a

  20. Annual Report of 49-2 SPR Operation in 2013

    Institute of Scientific and Technical Information of China (English)

    WANG; Yi-wei; ZHANG; Ya-dong; YANG; Xiao; ZHU; Lei

    2013-01-01

    1 Information of operation and production Operation person:present chief operators are 12 persons,operators are 3 persons;new chief operators are 0 persons,new operator is 0 person;training chief operators are 0 persons,training operators

  1. Annual Report of 49-2 SPR Operation in 2012

    Institute of Scientific and Technical Information of China (English)

    ZHANG; Ya-dong; NIU; Sheng-li

    2012-01-01

    <正>1 Information of operation and production Operation person: present chief operators are 13 persons, operators are 4 persons; new chief operators are 3 persons, new operator is 1 person; training chief operators are 0 persons, training operators are 0 persons; transferred chief operator is 1 person; transferred operator is 0 person. Operation data:

  2. Three dimensional simulation for Big Hill Strategic Petroleum Reserve (SPR).

    Energy Technology Data Exchange (ETDEWEB)

    Ehgartner, Brian L. (Sandia National Laboratories, Albuquerque, NM); Park, Byoung Yoon; Sobolik, Steven Ronald (Sandia National Laboratories, Albuquerque, NM); Lee, Moo Yul (Sandia National Laboratories, Albuquerque, NM)

    2005-07-01

    3-D finite element analyses were performed to evaluate the structural integrity of caverns located at the Strategic Petroleum Reserve's Big Hill site. State-of-art analyses simulated the current site configuration and considered additional caverns. The addition of 5 caverns to account for a full site and a full dome containing 31 caverns were modeled. Operations including both normal and cavern workover pressures and cavern enlargement due to leaching were modeled to account for as many as 5 future oil drawdowns. Under the modeled conditions, caverns were placed very close to the edge of the salt dome. The web of salt separating the caverns and the web of salt between the caverns and edge of the salt dome were reduced due to leaching. The impacts on cavern stability, underground creep closure, surface subsidence and infrastructure, and well integrity were quantified. The analyses included recently derived damage criterion obtained from testing of Big Hill salt cores. The results show that from a structural view point, many additional caverns can be safely added to Big Hill.

  3. Bryan Mound SPR cavern 113 remedial leach stage 1 analysis.

    Energy Technology Data Exchange (ETDEWEB)

    Rudeen, David Keith; Weber, Paula D.; Lord, David L.

    2013-08-01

    The U.S. Strategic Petroleum Reserve implemented the first stage of a leach plan in 2011-2012 to expand storage volume in the existing Bryan Mound 113 cavern from a starting volume of 7.4 million barrels (MMB) to its design volume of 11.2 MMB. The first stage was terminated several months earlier than expected in August, 2012, as the upper section of the leach zone expanded outward more quickly than design. The oil-brine interface was then re-positioned with the intent to resume leaching in the second stage configuration. This report evaluates the as-built configuration of the cavern at the end of the first stage, and recommends changes to the second stage plan in order to accommodate for the variance between the first stage plan and the as-built cavern. SANSMIC leach code simulations are presented and compared with sonar surveys in order to aid in the analysis and offer projections of likely outcomes from the revised plan for the second stage leach.

  4. Live cell refractometry based on non-SPR microparticle sensor.

    Science.gov (United States)

    Liu, Chang; Chen, David D Y; Yu, Lirong; Luo, Yong

    2013-06-01

    Unlike the nanoparticles with surface plasmon resonance, the optical response of polystyrene microparticles (PSMPs) is insensitive to the chemical components of the surrounding medium under the wavelength-dependent differential interference contrast microscopy. This fact is exploited for the measurement of the refractive index of cytoplasm in this study. PSMPs of 400 nm in diameter were loaded into the cell to contact cytoplasm seamlessly, and the refractive index information of cytoplasm could be extracted by differential interference contrast microscopy operated at 420 nm illumination wavelength through the contrast analysis of PSMPs images. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  5. Infrared SPR sensing with III-nitride dielectric layers

    OpenAIRE

    Núñez-Cascarejo, A.; Estéban, O.; Méndez, J.A,; M. González-Herráez; Naranjo, F. B.

    2016-01-01

    In this work, Aluminum Indium Nitride (AlxIn1-xN) has been used as the dielectric overlay for a surface plasmon resonance sensor. The use of a ternary compound such as AlxIn1-xN for the dielectric allows a fine tuning of its refractive index by varying its composition, thus improving the sensor performance. Narrower transmittance resonances and higher sensitivities are obtained for transducers where the substrate rotates while depositing the ternary compound, which is attributed to the deposi...

  6. Influence of metal roughness on SPR sensor performance

    Science.gov (United States)

    Agarwal, Sajal; Prajapati, Y. K.; Singh, V.

    2017-01-01

    Roughness of the nano-layer greatly affects the sensor performance. This study is done to quantify the effect of roughness on the sensor performance experimentally. It is seen that the increased thickness of the top metal layer degrades the sensor performance i.e. sensitivity and detection accuracy. The roughness effect on the surface is seen by varying the thickness of intermediate and top metal layers separately. It is seen that 2-5 nm thick intermediate layer and 50 nm thick top layer provides better performance of sensor. Also, mathematical equations are included for the sake of theoretical analysis which indicates the effect of surface roughness on the sensor performance.

  7. Beregnbarhet og implementasjon : - aspekter ved funksjonelle språk

    OpenAIRE

    Hanssen, Arne

    2008-01-01

    In this master thesis we investigate lambda calculus and the theory of combinatory logic. Two computational models strongly related to functional programming languages. The lambda calculus is a theoretical foundation and a framework for describing functions and their evaluation. The theory of combinatory logic can be viewed a theory of computation based on a notation that eliminates the need for variables in applications. We also study Lisp, the SECD-machine and graph reduction, highlighting ...

  8. Methods to immobilize GPCR on the surface of SPR sensors.

    Science.gov (United States)

    Martínez-Muñoz, Laura; Barroso, Rubén; Paredes, Anabel Guedán; Mellado, Mario; Rodríguez-Frade, José Miguel

    2015-01-01

    The G protein-coupled receptors (GPCRs) form one of the largest membrane receptor families. The nature of the ligands that interact with these receptors is highly diverse; they include light, peptides and hormones, neurotransmitters, and small molecular weight compounds. The GPCRs are involved in a wide variety of physiological processes and thus hold considerable therapeutic potential.GPCR function is usually determined in cell-based assays, whose complexity nonetheless limits their use. The use of alternative, cell-free assays is hampered by the difficulties in purifying these seven-transmembrane domain receptors without altering their functional properties. Several methods have been proposed to immobilize GPCR on biosensor surfaces which use antibodies or avidin-/biotin-based capture procedures, alone or with reconstitution of the GPCR physiological microenvironment. Here we propose a method for GPCR immobilization in their native membrane microenvironment that requires no manipulation of the target receptor and maintains the many conformations GPCR can adopt in the cell membrane.

  9. Surface plasmon resonance (SPR), analyses van cellen in apoptose

    NARCIS (Netherlands)

    Buijtenhuijs, P.; Sephat, C.; Poot, A.A.; Vermes, I.; Feijen, J.

    2002-01-01

    Inleiding: Apoptotische cellen geven expressie van phosphatidylserine (PS) aan de buitenkant van hun celmembraan. Annexine-V heeft in aanwezigheid van Ca2+ een sterke affiniteit voor PS. Dit verschijnsel wordt gebruikt voor het aantonen van cellen die in apoptose zijn door het meten van de mate van

  10. Einflussfaktoren auf das Sprödbruchverhalten im Fallgewichtsversuch

    OpenAIRE

    Scholl, Sebastian

    2014-01-01

    The drop weight test according to the Battelle Memorial Institute (Battelle Drop Weight Tear Test) is a standardized basis for the evaluation of crack arrest ability of linepipe steels. Similar to the Charpy V-Notch test a pre-notched specimen will be broken with a weighted fin. The ability for crack arrest is subsequently measured indirectly via the shear area percentage of the fracture surface. In contrast to the Charpy V-Notch test, the enlarged dimensions of the test in combination with t...

  11. Tromsø som samisk by? – Språkideologier og medienes rolle i språkdebatten

    Directory of Open Access Journals (Sweden)

    Florian Hiss

    2012-10-01

    Full Text Available This article presents a critical analysis of the discursive practices in the public debate on Sámi language in Tromsø. The conflict around the political plan of Tromsø municipality to join the administrative area for the Sámi language lasted for about one year and was largely  carried out in  the  local  newspapers,  which  had  established  themselves  as  an arena and broker in the conflict. The focus of this study is, on the one hand, on the role of the media in the debate and, on the other hand, on the socially constructed relations between Sámi and Norwegian language and social meanings, which get expressed in a highly  ideological  picture of  language  and  local  identity  and  form  the  ground  for the language conflict. The analytic strategy is twofold: as a first step, the study focuses on the reproduction of  language  ideologies  in  letters  to  the  editor  and readers’  contributions  to  the  local papers’  discussion  pages.  The identification  of  the  three  semiotic  processes  of iconization (rhematization,  fractal  recursivity,  and  erasure  reveals  how  the writers’ expressions of opinion are anchored in a language ideology that connects Sámi language with  certain  social  values  and  ignores  a larger  linguistic  and  cultural  diversity  in  the town of Tromsø. As a second step, the analysis explores the journalistic treatment of the multitude of conflicting voices in the debate and critically sheds light on the construction of a journalistic voice. Although the journalists claim to construe a seemingly neutral ground for their reports (or independent comments, the analysis shows that journalists use  the  representation of  various  voices  in  their  texts  to  construe  a positioned, evaluating, and  ideologically  anchored  journalistic  voice.  In  face  of  the  highly ideological character of the language debate in Tromsø, I argue that local journalism has failed in countering the ideological picture of language and society through information and independent journalism.

  12. Associations between early life experience, chronic HPA axis activity, and adult social rank in rhesus monkeys.

    Science.gov (United States)

    Dettmer, Amanda M; Wooddell, Lauren J; Rosenberg, Kendra L; Kaburu, Stefano S K; Novak, Melinda A; Meyer, Jerrold S; Suomi, Stephen J

    2017-02-01

    Early life experience and socioeconomic status (SES) are well-established predictors of health outcomes in people. Both factors likely influence health outcomes via hypothalamic-pituitary-adrenal (HPA) axis regulation. However, it is unclear how early experience and HPA axis activity influence adult social status. We studied differentially reared female rhesus monkeys (Macaca mulatta, N = 90) as models to test the hypothesis that chronic HPA axis activity assessed via hair cortisol concentrations (HCCs) mediated the relationship between early life experience and adult social rank. We found that mother-peer-reared (MPR) monkeys acquired higher social ranks than either of the two nursery-reared (NR) groups (peer-reared, PR, or surrogate-peer-reared, SPR monkeys) (β = -0.07, t(89) = -2.16, p = 0.034). We also found that MPR HCCs were lower during the juvenile period at 18 months (F(2,25) = 3.49, p = 0.047). Furthermore, for MPR but not NR monkeys, changes in HCCs from 18 to 24 months (r(s) = -0.627, p = 0.039) and adult HCCs (r(s) = -0.321, p = 0.03) were negatively correlated with adult social rank. These findings suggest that chronic HPA axis regulation in juvenility, and perhaps in adulthood, may influence adult social status for primates that experience typical early rearing. However, early life adversity may result in dissociation between neuroendocrine stress regulation and adult social competence, which may be risk factors for adverse health outcomes.

  13. Experiment Dashboard for the LHC Experiments

    CERN Document Server

    Andreeva, Julia; Gaidioz, Benjamin; Herrala, Juha; Maier, Gerhild; Rocha, Ricardo; Saiz, Pablo; Sidorova, Irina; CERN. Geneva. IT Department

    2007-01-01

    The goal of the Grid is to provide a coherent access to distributed computing resources. All LHC experiments are using several Grid infrastructures and a variety of the middleware flavors. Due to the complexity and heterogeinity of a distributed system the monitoring represents a challenging task. Independently of the underlying platform , the experiments need to ave a complete and uniform picture of their activities on the Grid ideally seen by the users as a single powerful computing resource. Overall operation of the infrastructure used by experiments is defined both by the quality of the Grid and the quality of the tools and services developed/used by the experiments. Correspondingly the required monitoring information should combine both Grid-related and experiment/application specific data. On the other hand, users of the LHC experiments have various roles and need different levels of details regarding monitoring data. The paper will focus on the Grid monitoring from the experiment/user perspectives with...

  14. The User Experience

    Science.gov (United States)

    Schmidt, Aaron

    2010-01-01

    User experience (UX) is about arranging the elements of a product or service to optimize how people will interact with it. In this article, the author talks about the importance of user experience and discusses the design of user experiences in libraries. He first looks at what UX is. Then he describes three kinds of user experience design: (1)…

  15. The User Experience

    Science.gov (United States)

    Schmidt, Aaron

    2010-01-01

    User experience (UX) is about arranging the elements of a product or service to optimize how people will interact with it. In this article, the author talks about the importance of user experience and discusses the design of user experiences in libraries. He first looks at what UX is. Then he describes three kinds of user experience design: (1)…

  16. Real Life Experiences with Experience Design

    DEFF Research Database (Denmark)

    Dalsgård, Peter; Halskov, Kim

    2006-01-01

    Experience Design is an emergent field of study, and various approaches to the field abound. In this paper, we take a pragmatic approach to identifying key aspects of an experience design process, by reporting on a project involving the design of experience-oriented applications of interactive...... technologies for knowledge dissemination and marketing, in cooperation with public institutions and businesses. We argue that collaborative formulation of core design intentions and values is a valuable instrument in guiding experience design processes, and present three cases from this project, two of which...... the installations, the core values established to guide the design process and the intended use contexts. We argue that the installations present a broad spectrum of experience design installations that can assist designers in understanding the relations between core values, intentions, use context and interface...

  17. Structural Assembly Demonstration Experiment (SADE) experiment design

    Science.gov (United States)

    Akin, D. L.; Bowden, M. L.

    1982-03-01

    The Structural Assembly Demonstration Experiment concept is to erect a hybrid deployed/assembled structure as an early space experiment in large space structures technology. The basic objectives can be broken down into three generic areas: (1) by performing assembly tasks both in space and in neutral buoyancy simulation, a mathematical basis will be found for the validity conditions of neutral buoyancy, thus enhancing the utility of water as a medium for simulation of weightlessness; (2) a data base will be established describing the capabilities and limitations of EVA crewmembers, including effects of such things as hardware size and crew restraints; and (3) experience of the M.I.T. Space Systems Lab in neutral buoyancy simulation of large space structures assembly indicates that the assembly procedure may create the largest loads that a structure will experience during its lifetime. Data obtained from the experiment will help establish an accurate loading model to aid designers of future space structures.

  18. The Social Experiment Market

    OpenAIRE

    David Greenberg; Mark Shroder; Matthew Onstott

    1999-01-01

    In social experiments, individuals, households, or organizations are randomly assigned to two or more policy interventions. Elsewhere, we have summarized 143 experiments completed by autumn 1996. Here, we use the information we have gathered on these experiments and findings from informal telephone interviews to investigate the social experiment market--the buyers and sellers in the market that governs the production of experiments. We discuss target populations, types of interventions tested...

  19. Adaptive structures flight experiments

    Science.gov (United States)

    Martin, Maurice

    The topics are presented in viewgraph form and include the following: adaptive structures flight experiments; enhanced resolution using active vibration suppression; Advanced Controls Technology Experiment (ACTEX); ACTEX program status; ACTEX-2; ACTEX-2 program status; modular control patch; STRV-1b Cryocooler Vibration Suppression Experiment; STRV-1b program status; Precision Optical Bench Experiment (PROBE); Clementine Spacecraft Configuration; TECHSAT all-composite spacecraft; Inexpensive Structures and Materials Flight Experiment (INFLEX); and INFLEX program status.

  20. Popper's Thought Experiment Reinvestigated

    CERN Document Server

    Richardson, Chris D

    2011-01-01

    Popper's original thought experiment probed some fundamental and subtle rules of quantum mechanics. Two recent experiments have tested Popper's hypothesis, but they seem to give contrasting results and one suggests a violation of Heisenberg's uncertainty principle. The equations governing these two experiments and Popper's thought experiment will be derived from basic principles. The experimental constants will be inputted and it will show that the two experiments agree with each other. It will be shown that no uncertainty relations are violated and that Popper's thought experiment was fundamentally flawed.

  1. Experiment WA1 (CDHS Neutrino Experiment)

    CERN Multimedia

    1977-01-01

    Experiment WA1, also known under CDHS (CERN, Dortmund, Heidelberg, Saclay; spokesman Jack Steinberger), was the first neutrino experiment on the SPS, in its West Area. Magnetized iron (with a toroidal field) forms the core of the detector. On its outside we see drift chambers and photomultipliers (detecting the light from the plastic scintillators further in). Peter Schilly is wearing a white coat. See also CERN Annual Report 1976, p.57.

  2. The AMS experiment

    CERN Multimedia

    Laurent Guiraud

    1999-01-01

    The Alpha Magnetic Spectrometer (AMS) detector will be installed as a particle physics experiment on the International Space Station. It will look for antimatter pockets in space. AMS is a CERN recognised experiment.

  3. Future Solar Neutrino Experiments

    Energy Technology Data Exchange (ETDEWEB)

    Nakahata, M. [Kamioka Observatory, Institute for Cosmic Ray research, University of Tokyo, Higashi-Mozumi, Kamioka-cho, Hida-shi, Gifu, Japan, 506-1205 (Japan)]. E-mail: nakahata@suketto.icrr.u-tokyo.ac.jp

    2005-08-15

    The value of future solar neutrino experiments is discussed from particle physics and astrophysics points of view based on current understanding of solar neutrino oscillations. R and D statuses of future experiments are also discussed.

  4. Experiments in Magnetohydrodynamics

    Science.gov (United States)

    Rayner, J. P.

    1970-01-01

    Describes three student experiments in magnetohydrodynamics (MHD). In these experiments, it was found that the electrical conductivity of the local water supply was sufficient to demonstrate effectively some of the features of MHD flowmeters, generators, and pumps. (LC)

  5. Determination of vitamin B12 in fortified bovine milk-based infant formula powder, fortified soya-based infant formula powder, vitamin premix, and dietary supplements by surface plasmon resonance: collaborative study.

    Science.gov (United States)

    Vyas, Pathik; O'Kane, Anthony A

    2011-01-01

    A collaborative study was conducted on an inhibition-based protein-binding assay using the Biacore Q biosensor instrument and the Biacore Qflex Kit Vitamin B12 PI. The samples studied included infant formula, cereals, premixes, vitamin tablets, dietary supplements, and baby food. The collaborative study, which involved 11 laboratories, demonstrated that the assay showed an RSDr of 1.59-27.8 and HorRat values for reproducibility of 0.34-1.89 in samples with levels ranging from ppm to ppb. The assay studied is a label-free protein binding-based assay that uses the principle of surface plasmon resonance (SPR) to measure the interaction between vitamin B12 and a specific binding protein. A Biacore Q biosensor uses this principle to detect binding directly at the surface of a sensor chip with a hydrophilic gold-dextran surface. The instrument passes a mixture of prepared sample extract and binding protein solution across a covalently immobilized vitamin B12 chip surface, and the response is given as free-binding protein as the mixture binds to the immobilized surface. This technique uses the specificity and robustness of the protein-ligand interaction to allow minimal sample preparation and a wide range of matrixes to be analyzed rapidly. The reagents and accessories needed to perform this assay are provided as the ready-to-use format "Qflex Kit Vitamin B12 PI." The method is intended for routine use in the quantitative determination of vitamin B12 (as cyanocobalamin) in a wide range of food products, dietary vitamin supplements, and multivitamin premixes.

  6. Prioritized Experience Replay

    OpenAIRE

    Schaul, Tom; Quan, John; Antonoglou, Ioannis; Silver, David

    2015-01-01

    Experience replay lets online reinforcement learning agents remember and reuse experiences from the past. In prior work, experience transitions were uniformly sampled from a replay memory. However, this approach simply replays transitions at the same frequency that they were originally experienced, regardless of their significance. In this paper we develop a framework for prioritizing experience, so as to replay important transitions more frequently, and therefore learn more efficiently. We u...

  7. Customer Experience Management Overview

    OpenAIRE

    Havíř, David

    2016-01-01

    Purpose of the article: The purpose of this paper is to examine the phenomenon of customer experience and customer experience management through years and summarize recent knowledge in this area. Methodology/methods: The paper is built upon secondary research of research papers of renowned authors in the area of customer experience management. Scientific aim: The aim of the article is to find out potential avenues of further research. Findings: Findings confirmed that customer experience is s...

  8. Organic chemistry experiment

    Energy Technology Data Exchange (ETDEWEB)

    Mun, Seok Sik

    2005-02-15

    This book deals with organic chemistry experiments, it is divided five chapters, which have introduction, the way to write the experiment report and safety in the laboratory, basic experiment technic like recrystallization and extraction, a lot of organic chemistry experiments such as fischer esterification, ester hydrolysis, electrophilic aromatic substitution, aldol reaction, benzoin condensation, wittig reaction grignard reaction, epoxidation reaction and selective reduction. The last chapter introduces chemistry site on the internet and way to find out reference on chemistry.

  9. Visual experience and blindsight

    DEFF Research Database (Denmark)

    Overgaard, Morten

    2011-01-01

    Blindsight is classically defined as residual visual capacity, e.g., to detect and identify visual stimuli, in the total absence of perceptual awareness following lesions to V1. However, whereas most experiments have investigated what blindsight patients can and cannot do, the literature contains...... several, often contradictory, remarks about remaining visual experience. This review examines closer these remarks as well as experiments that directly approach the nature of possibly spared visual experiences in blindsight....

  10. The Experiment Factory: standardizing behavioral experiments

    Directory of Open Access Journals (Sweden)

    Vanessa V Sochat

    2016-04-01

    Full Text Available The administration of behavioral and experimental paradigms for psychology research is hindered by lack of a coordinated effort to develop and deploy standardized paradigms. While several frameworks (de Leeuw (2015; McDonnell et al. (2012; Mason and Suri (2011; Lange et al. (2015 have provided infrastructure and methods for individual research groups to develop paradigms, missing is a coordinated effort to develop paradigms linked with a system to easily deploy them. This disorganization leads to redundancy in development, divergent implementations of conceptually identical tasks, disorganized and error-prone code lacking documentation, and difficulty in replication. The ongoing reproducibility crisis in psychology and neuroscience research (Baker (2015; Open Science Collaboration (2015 highlights the urgency of this challenge: reproducible research in behavioral psychology is conditional on deployment of equivalent experiments. A large, accessible repository of experiments for researchers to develop collaboratively is most efficiently accomplished through an open source framework. Here we present the Experiment Factory, an open source framework for the development and deployment of web-based experiments. The modular infrastructure includes experiments, virtual machines for local or cloud deployment, and an application to drive these components and provide developers with functions and tools for further extension. We release this infrastructure with a deployment (http://www.expfactory.org that researchers are currently using to run a set of over 80 standardized web-based experiments on Amazon Mechanical Turk. By providing open source tools for both deployment and development, this novel infrastructure holds promise to bring reproducibility to the administration of experiments, and accelerate scientific progress by providing a shared community resource of psychological paradigms.

  11. The Experiment Factory: Standardizing Behavioral Experiments

    Science.gov (United States)

    Sochat, Vanessa V.; Eisenberg, Ian W.; Enkavi, A. Zeynep; Li, Jamie; Bissett, Patrick G.; Poldrack, Russell A.

    2016-01-01

    The administration of behavioral and experimental paradigms for psychology research is hindered by lack of a coordinated effort to develop and deploy standardized paradigms. While several frameworks (Mason and Suri, 2011; McDonnell et al., 2012; de Leeuw, 2015; Lange et al., 2015) have provided infrastructure and methods for individual research groups to develop paradigms, missing is a coordinated effort to develop paradigms linked with a system to easily deploy them. This disorganization leads to redundancy in development, divergent implementations of conceptually identical tasks, disorganized and error-prone code lacking documentation, and difficulty in replication. The ongoing reproducibility crisis in psychology and neuroscience research (Baker, 2015; Open Science Collaboration, 2015) highlights the urgency of this challenge: reproducible research in behavioral psychology is conditional on deployment of equivalent experiments. A large, accessible repository of experiments for researchers to develop collaboratively is most efficiently accomplished through an open source framework. Here we present the Experiment Factory, an open source framework for the development and deployment of web-based experiments. The modular infrastructure includes experiments, virtual machines for local or cloud deployment, and an application to drive these components and provide developers with functions and tools for further extension. We release this infrastructure with a deployment (http://www.expfactory.org) that researchers are currently using to run a set of over 80 standardized web-based experiments on Amazon Mechanical Turk. By providing open source tools for both deployment and development, this novel infrastructure holds promise to bring reproducibility to the administration of experiments, and accelerate scientific progress by providing a shared community resource of psychological paradigms. PMID:27199843

  12. Double beta decay experiments

    CERN Document Server

    Barabash, A S

    2011-01-01

    The present status of double beta decay experiments is reviewed. The results of the most sensitive experiments are discussed. Proposals for future double beta decay experiments with a sensitivity to the $$ at the level of (0.01--0.1) eV are considered.

  13. The Concerned Observer Experiment.

    Science.gov (United States)

    Rabiger, Michael

    1991-01-01

    Describes a classroom experiment--the "concerned observer" experiment--for production students that dramatizes basic film language by relating it to several levels of human observation. Details the experiment's three levels, and concludes that film language mimics wide-ranging states of human emotion and ideological persuasion. (PRA)

  14. The Concerned Observer Experiment.

    Science.gov (United States)

    Rabiger, Michael

    1991-01-01

    Describes a classroom experiment--the "concerned observer" experiment--for production students that dramatizes basic film language by relating it to several levels of human observation. Details the experiment's three levels, and concludes that film language mimics wide-ranging states of human emotion and ideological persuasion. (PRA)

  15. Designing Urban Experiences

    DEFF Research Database (Denmark)

    Jantzen, Christian; Vetner, Mikael

    2008-01-01

    traditional urban planning aspects such as infrastructure, environmental factors and aesthetics, but has also dealt with the design of urban experiences. Through an introduction of the framework of the structure of experiences, this article examines how urban experiences can be understood and analysed...

  16. Leisure experience and imagination

    NARCIS (Netherlands)

    Lengkeek, J.

    2001-01-01

    Holiday-makers experience the places where they are on holiday in different ways. Back in 1979, Erik Cohen introduced his 'modes of tourist experience'. Cohen's approach was promising for better understanding 'experiences' in a phenomenological way but very little happened afterwards with his 'modes

  17. Experiments in physical chemistry

    CERN Document Server

    Wilson, J M; Denaro, A R

    1968-01-01

    Experiments in Physical Chemistry, Second Edition provides a compilation of experiments concerning physical chemistry. This book illustrates the link between the theory and practice of physical chemistry. Organized into three parts, this edition begins with an overview of those experiments that generally have a simple theoretical background. Part II contains experiments that are associated with more advanced theory or more developed techniques, or which require a greater degree of experimental skill. Part III consists of experiments that are in the nature of investigations wherein these invest

  18. Future Outlook: Experiment

    Energy Technology Data Exchange (ETDEWEB)

    Suzuki, Yoichiro [Kamioka, Observatory, Institute for Cosmic Ray Research, University of Tokyo, and Institute for the Physics and Mathematics of the Universe, University of Tokyo. 456 Higashi-Mozumi, Kamioka, Hida-city, Gifu, 506-1205 Japan (Japan)], E-mail: suzuki@suketto.icrr.u-tokyo.ac.jp

    2008-11-01

    The personal view for the next to the next neutrino detector, the ultimate experiment, is discussed. Considering the size, cost and head winds against the basic science, the ultimate experiment will be the only experiment in the world. Here two such experiments one for the neutrino oscillation and the other for the double beta decay were discussed. The ultimate experiment needs to include a bread and butter science and to have a discovery potential for an unexpected phenomenon. There are many technical challenges and international co-operations are absolutely necessary.

  19. Virtual neutron scattering experiments

    DEFF Research Database (Denmark)

    Overgaard, Julie Hougaard; Bruun, Jesper; May, Michael

    2017-01-01

    We describe how virtual experiments can be utilized in a learning design that prepares students for hands-on experiments at large-scale facilities. We illustrate the design by showing how virtual experiments are used at the Niels Bohr Institute in a master level course on neutron scattering....... In the last week of the course, students travel to a large-scale neutron scattering facility to perform real neutron scattering experiments. Through student interviews and survey answers, we argue, that the virtual training prepares the students to engage more fruitfully with experiments by letting them focus...... on physics and data rather than the overwhelming instrumentation. We argue that this is because they can transfer their virtual experimental experience to the real-life situation. However, we also find that learning is still situated in the sense that only knowledge of particular experiments is transferred...

  20. Virtual neutron scattering experiments

    DEFF Research Database (Denmark)

    Overgaard, Julie Hougaard; Bruun, Jesper; May, Michael

    2016-01-01

    We describe how virtual experiments can be utilized in a learning design that prepares students for hands-on experiments at large-scale facilities. We illustrate the design by showing how virtual experiments are used at the Niels Bohr Institute in a master level course on neutron scattering....... In the last week of the course, students travel to a large-scale neutron scattering facility to perform real neutron scattering experiments. Through student interviews and survey answers, we argue, that the virtual training prepares the students to engage more fruitfully with experiments by letting them focus...... on physics and data rather than the overwhelming instrumentation. We argue that this is because they can transfer their virtual experimental experience to the real-life situation. However, we also find that learning is still situated in the sense that only knowledge of particular experiments is transferred...

  1. Experiments with Individual Photons

    Science.gov (United States)

    Beck, Mark

    2004-05-01

    I describe several different experiments we have performed with individual photons. For example, while well known experiments involving phenomena such as the photoelectric effect and Compton scattering strongly suggest the existence of photons, they do not prove the existence of light quanta. To prove the existence of light quanta one must perform an experiment whose results cannot be explained using classical waves. We have performed such an experiment--it demonstrates the localization of light quanta by showing that a single photon only goes one way when it leaves a beamsplitter [1]. In a second experiment we demonstrate that this single photon will interfere with itself when it transits an interferometer. The experiments have been performed by undergraduates, and the goal of this project is to develop a series of experiments exploring fundamental aspects of quantum mechanics for an undergraduate teaching lab. [1] P. Grangier, G. Roger and A. Aspect, Europhys. Lett. 1, 173 (1986).

  2. Social experience infrastructure

    DEFF Research Database (Denmark)

    Kvistgaard, Peter

    2006-01-01

    Using the case of Kühlungsborn in Mecklenburg-Vorpommern as an example of a resort in which social experience infrastructure plays a pivotal role in the ongoing success of attracting German tourists from especially Berlin, Hamburg and Hanover it is the aim of this article in a descriptive...... and explorative fashion to share with others thoughts and ideas concerning the development of new ways to construct/reconstruct recreational spaces with a better coherence with regard to designing experiences. This article claims that it is possible to design recreational spaces with good social experience...... infrastructure in order to create experience spaces for personal experiences (in line with Schultze’s social constructivist view of experiences) without completely adhering to the economic rationalist thoughts and guidelines of Pine & Gilmore that claim that experiences can be designed and controlled...

  3. Popper's Thought Experiment Reinvestigated

    Science.gov (United States)

    Richardson, Chris; Dowling, Jonathan

    2012-02-01

    Karl Popper posed an interesting thought experiment in 1934. With it, he meant to question the completeness of quantum mechanics. He claimed that the notion of quantum entanglement leads to absurd scenarios that cannot be true in real life and that an implementation of his thought experiment would not give the results that QM predicts. Unfortunately for Popper, it has taken until recently to perform experiments that test his claims. The results of the experiments do not refute QM as Popper predicted, but neither do they confirm what Popper claimed QM predicted. Kim and Shih implemented Popper's thought experiment in the lab. The results of the experiment are not clear and have instigated many interpretations of the results. The results show some correlation between entangled photons, but not in the way that Popper thought, nor in the way a simple application of QM might predict. A ghost-imaging experiment by Strekalov, et al. sheds light on the physics behind Popper's thought experiment, but does not try to directly test it. I will build the physics of Popper's thought experiment from the ground up and show how the results of both of these experiments agree with each other and the theory of QM, but disprove Popper.

  4. Experiment, right or wrong

    CERN Document Server

    Franklin, Allan

    2008-01-01

    In Experiment, Right or Wrong, Allan Franklin continues his investigation of the history and philosophy of experiment presented in his previous book, The Neglect of Experiment. In this new study, Franklin considers the fallibility and corrigibility of experimental results and presents detailed histories of two such episodes: 1) the experiment and the development of the theory of weak interactions from Fermi's theory in 1934 to the V-A theory of 1957 and 2) atomic parity violation experiments and the Weinberg-Salam unified theory of electroweak interactions of the 1970s and 1980s. In these episodes Franklin demonstrates not only that experimental results can be wrong, but also that theoretical calculations and the comparison between experiment and theory can also be incorrect. In the second episode, Franklin contrasts his view of an "evidence model" of science in which questions of theory choice, confirmation, and refutation are decided on the basis of reliable experimental evidence, with that proposed by the ...

  5. Reactor Neutrino Experiments

    OpenAIRE

    Cao, Jun

    2007-01-01

    Precisely measuring $\\theta_{13}$ is one of the highest priority in neutrino oscillation study. Reactor experiments can cleanly determine $\\theta_{13}$. Past reactor neutrino experiments are reviewed and status of next precision $\\theta_{13}$ experiments are presented. Daya Bay is designed to measure $\\sin^22\\theta_{13}$ to better than 0.01 and Double Chooz and RENO are designed to measure it to 0.02-0.03. All are heading to full operation in 2010. Recent improvements in neutrino moment measu...

  6. Qualitative experiments in psychology

    DEFF Research Database (Denmark)

    Wagoner, Brady

    2015-01-01

    In this article, I explore the meaning of experiments in early twentieth century psychology, focusing on the qualitative experimental methodology of psychologist Frederic BARTLETT. I begin by contextualizing BARTLETT's experiments within the continental research tradition of his time, which...... and extensions" of BARTLETT's experiments, demonstrating how his methodology was progressively changed and misunderstood over time. An argument is made for re-introducing an open, qualitative and idiographic experimental method similar to the one BARTLETT practiced....

  7. Experience Communication and Aesthetics

    DEFF Research Database (Denmark)

    Thorlacius, Lisbeth

    In this article the term "experience communication" will be introduced and discussed. It will be illustrated how different concepts of aesthetical experiences are an integrated part of experience communication and how these concepts are produced within the industries of consumerism, branding...... which was a given to something which each human being has to manage individually. As a consequence the human being experiences an increasing sense of insecurity and restless seeking after identity and recognition, which is reflected in an increasing demand of among other things self...

  8. Designing learning experiences

    National Research Council Canada - National Science Library

    Collins, Jannette

    2007-01-01

    Creation of significant learning experiences follows basic steps of instructional design related to situational factors, goals and objectives, feedback and evaluation methods, teaching and learning...

  9. Linking consumer experiences

    DEFF Research Database (Denmark)

    Smed, Karina Madsen

    Consumers consume products in various ways serving a number of purposes. Much attention has been paid to experiences attached to consumption, sometimes very explicitly, e.g. in tourism, the essence of which is experiences of various sorts, but often also implicitly as internalised experiences...... become part of the individual self, worldview, and behaviour. This paper seeks to explore links between consumer experiences through the exploration of narrative sequences in travel blogs. Findings indicate that non-consumption is a central element to the bloggers and also indicative of a community...

  10. 苯并噻唑希夫碱类化合物的合成及其与β-淀粉样蛋白结合性能的研究%Synthesis of benzothiazole derivatives and their binding characteristics with β-amyloid

    Institute of Scientific and Technical Information of China (English)

    周琳; 甘昌胜; 汪昊曙; 赵珍珍; 潘见

    2012-01-01

    Alzheimer's disease (AD) is a progressive neurodegenerative disease endangering human health seriously. Recent reports have revealed that β-amyloid aggregates play a key role in the pathogenesis of AD. Thus, targeting the Aβ plaques benzothiazole derivatives were synthesized with the scaffold of the most promising imaging agent PIB ([11C]-6-OH-BTA-l, ["C]-2-(4-(raethylamino)phenyl)-6-hydroxybenzothiazole) and C=N as linker to study the binding characteristics with the target protein through surface plasmon resonance (SPR) technique. These derivatives were synthesized through simple yet effective method with high yields and characterized by 'H NMR and FUR. The binding properties {KD} were determined with Biacore X-100 instrument according to the fitting-plot curve. Compounds 3a and 3f showed high binding affinity for Aβ1-40. The results suggest that benzothiazole derivatives could be served as a scaffold to develop novel β-amyloid imaging agents for the diagnosis of AD.

  11. Teaching Knowledge Engineering: Experiences

    DEFF Research Database (Denmark)

    Andersen, Tom; Hartvig, Susanne C

    1998-01-01

    Includes description of experiences gained by teaching KE in construction domains. It outlines good starting points and overall guidance to education in applied AI.......Includes description of experiences gained by teaching KE in construction domains. It outlines good starting points and overall guidance to education in applied AI....

  12. On the Poggendorff Experiment

    Science.gov (United States)

    Coelho, Ricardo Lopes; Silva, P. A. S.; Borges, Paulo de Faria

    2015-01-01

    Poggendorff showed experimentally, in the middle of the 19th century, that the weight of an Atwood machine is reduced when it is brought to motion. His experiment has been revisited from time to time, making use of instrumentation that reflects the technological development of the moment. In this paper, the evolution of the experiment is briefly…

  13. Fluorescence Experiments with Quinine

    Science.gov (United States)

    O'Reilly, James E.

    1975-01-01

    Describes a series of experiments which illustrate the analytical capabilities of fluorescence, and outlines two straightforward analyses involving real analyses. These experiments are suitable for an undergraduate instrumental analysis course and require approximately six to seven hours of laboratory time. (MLH)

  14. Real-World Experiences

    Science.gov (United States)

    Borja, Rhea R.

    2006-01-01

    This article presents IISME, a U.S. program that can give educators a real-world experience and that can deepen their subject-matter knowledge. It also presents the experiences of some teachers who are into this program. IISME's summer-fellowship program started out with 40 teachers and 12 companies. The group's growth picked up in 2001, when it…

  15. Peak Experience Project

    Science.gov (United States)

    Scott, Daniel G.; Evans, Jessica

    2010-01-01

    This paper emerges from the continued analysis of data collected in a series of international studies concerning Childhood Peak Experiences (CPEs) based on developments in understanding peak experiences in Maslow's hierarchy of needs initiated by Dr Edward Hoffman. Bridging from the series of studies, Canadian researchers explore collected…

  16. The French experience

    CERN Document Server

    Bougard, Marie-Thérèse

    2003-01-01

    Developed for beginners, The French Experience 1 course book is designed to accompany the French Experience 1 CDs (9780563472582) but can also be used on its own to develop your reading and writing skills. You’ll gain valuable insights into French culture too.

  17. User Experience Dimensions

    DEFF Research Database (Denmark)

    Lykke, Marianne; Jantzen, Christian

    2016-01-01

    The present study develops a set of 10 dimensions based on a systematic understanding of the concept of experience as a holistic psychological. Seven of these are derived from a psychological conception of what experiencing and experiences are. Three supplementary dimensions spring from the obser...

  18. New neutrino experiments

    Indian Academy of Sciences (India)

    Maury Goodman

    2004-02-01

    Following incredible recent progress in understanding neutrino oscillations, many new ambitious experiments are being planned to study neutrino properties. The most important may be to find a non-zero value of $_{13}$. The most promising way to do this appears to be to measure $_{}→ _{}$ oscillations with an $E/L$ near $ m^{2}_{\\text{atmo}}$. Future neutrino experiments are great.

  19. Experiments with Succinct Solvers

    DEFF Research Database (Denmark)

    Buchholtz, Mikael; Nielson, Hanne Riis; Nielson, Flemming

    2002-01-01

    time of the solver and the aim of this note is to provide some insight into which formulations are better than others. The experiments addresses three general issues: (i) the order of the parameters of relations, (ii) the order of conjuncts in preconditions and (iii) the use of memoisation....... The experiments are performed for Control Flow Analyses for Discretionary Ambients....

  20. Experience as Excursion

    DEFF Research Database (Denmark)

    Svabo, Connie; Shanks, Michael

    2014-01-01

    qualities of experience are highlighted as part of a suggestion that design may indeed relate as much to metaphysics as to mechanics, materials science, and the psychology of the consumer and user. An Experience Design is sketched out as the choreography of temporary and shifting engagements across...

  1. Play as Experience

    Science.gov (United States)

    Henricks, Thomas S.

    2015-01-01

    The author investigates what he believes one of the more important aspects of play--the experience it generates in its participants. He considers the quality of this experience in relation to five ways of viewing play--as action, interaction, activity, disposition, and within a context. He treats broadly the different forms of affect, including…

  2. Realisation, experience, and purification

    DEFF Research Database (Denmark)

    Sobisch, Jan-Ulrich

    2013-01-01

    Often Buddhist mantra practises are portrayed as making and maintaining meditation experiences. Jigten Sumgön explains in his dGongs gcig (5.19) that such a 'making and maintaining' is only a mental fabrication and needs to be purified. Realisation does not arise from experience, but from...

  3. Experiments as politics

    NARCIS (Netherlands)

    Spears, R; Smith, HJ

    2001-01-01

    The purpose of this paper is to illustrate the political nature of laboratory experiments. Such experiments can be construed as paradigms of power, open to construction and debate, where different agents and interests are involved in a process of struggle over both (re)presentation and substance. Ex

  4. Franklin: User Experiences

    Energy Technology Data Exchange (ETDEWEB)

    National Energy Research Supercomputing Center; He, Yun (Helen); Kramer, William T.C.; Carter, Jonathan; Cardo, Nicholas

    2008-05-07

    The newest workhorse of the National Energy Research Scientific Computing Center is a Cray XT4 with 9,736 dual core nodes. This paper summarizes Franklin user experiences from friendly early user period to production period. Selected successful user stories along with top issues affecting user experiences are presented.

  5. On the Poggendorff Experiment

    Science.gov (United States)

    Coelho, Ricardo Lopes; Silva, P. A. S.; Borges, Paulo de Faria

    2015-01-01

    Poggendorff showed experimentally, in the middle of the 19th century, that the weight of an Atwood machine is reduced when it is brought to motion. His experiment has been revisited from time to time, making use of instrumentation that reflects the technological development of the moment. In this paper, the evolution of the experiment is briefly…

  6. Experiments as politics

    NARCIS (Netherlands)

    Spears, R; Smith, HJ

    2001-01-01

    The purpose of this paper is to illustrate the political nature of laboratory experiments. Such experiments can be construed as paradigms of power, open to construction and debate, where different agents and interests are involved in a process of struggle over both (re)presentation and substance. Ex

  7. Expectations in experiments

    NARCIS (Netherlands)

    Wagener, F.

    2013-01-01

    The rational expectations hypothesis is one of the cornerstones of current economic theorising. this review discusses a number of experiments that focus on expectation formation by human subjects and analyses the implications for the rational expectations hypothesis. The experiments show that most a

  8. ATLAS Experiment Brochure

    CERN Multimedia

    AUTHOR|(INSPIRE)INSPIRE-00085461

    2016-01-01

    ATLAS is one of the four major experiments at the Large Hadron Collider at CERN. It is a general-purpose particle physics experiment run by an international collaboration, and is designed to exploit the full discovery potential and the huge range of physics opportunities that the LHC provides.

  9. Neutrino oscillation experiments

    Energy Technology Data Exchange (ETDEWEB)

    Camilleri, L. [European Organization for Nuclear Research, Geneva (Switzerland)

    1996-11-01

    Neutrino oscillation experiments ({nu}{sub {mu}}{yields}{nu}{sub e} and {nu}{sub {mu}}{yields}{nu}{sub {tau}}) currently being performed at accelerators are reviewed. Future plans for short and long base-line experiments are summarized. (author) 10 figs., 2 tabs., 29 refs.

  10. Peak Experience Project

    Science.gov (United States)

    Scott, Daniel G.; Evans, Jessica

    2010-01-01

    This paper emerges from the continued analysis of data collected in a series of international studies concerning Childhood Peak Experiences (CPEs) based on developments in understanding peak experiences in Maslow's hierarchy of needs initiated by Dr Edward Hoffman. Bridging from the series of studies, Canadian researchers explore collected…

  11. Future Solar Neutrino Experiments

    Energy Technology Data Exchange (ETDEWEB)

    Suzuki, Y. [Kamioka Observatory, Institute for Cosmic Ray Research, University of Tokyo, Higashi-Mozumi, Kamioka-cho, Hida-city, 506-1205 (Japan)]. E-mail: suzuki@suketto.icrr.u-tokyo.ac.jp

    2005-06-15

    The purpose of the future solar neutrino experiments is briefly reviewed. The future experimental programs which aim to measure the low energy solar neutrinos are described. We do not cover all the projects. Experiments using noble gases are promising for the pp-neutrino measurements.

  12. Making the Experience City

    DEFF Research Database (Denmark)

    Jensen, Ole B.

    2009-01-01

    This paper describes the latest research into cultural planning and architectural branding in Denmark based on the ‘Experience City' research project located at Aalborg University. The paper explores the implication of the turn towards culture and experience in the contemporary Danish city. It thus...

  13. A Duplicate Construction Experiment.

    Science.gov (United States)

    Bridgeman, Brent

    This experiment was designed to assess the ability of item writers to construct truly parallel tests based on a "duplicate-construction experiment" in which Cronbach argues that if the universe description and sampling are ideally refined, the two independently constructed tests will be entirely equivalent, and that within the limits of item…

  14. Teaching Knowledge Engineering: Experiences

    DEFF Research Database (Denmark)

    Andersen, Tom; Hartvig, Susanne C

    1998-01-01

    Includes description of experiences gained by teaching KE in construction domains. It outlines good starting points and overall guidance to education in applied AI.......Includes description of experiences gained by teaching KE in construction domains. It outlines good starting points and overall guidance to education in applied AI....

  15. Space Experiment Module (SEM)

    Science.gov (United States)

    Brodell, Charles L.

    1999-01-01

    The Space Experiment Module (SEM) Program is an education initiative sponsored by the National Aeronautics and Space Administration (NASA) Shuttle Small Payloads Project. The program provides nationwide educational access to space for Kindergarten through University level students. The SEM program focuses on the science of zero-gravity and microgravity. Within the program, NASA provides small containers or "modules" for students to fly experiments on the Space Shuttle. The experiments are created, designed, built, and implemented by students with teacher and/or mentor guidance. Student experiment modules are flown in a "carrier" which resides in the cargo bay of the Space Shuttle. The carrier supplies power to, and the means to control and collect data from each experiment.

  16. Experience as Excursion

    DEFF Research Database (Denmark)

    Svabo, Connie; Shanks, Michael

    2014-01-01

    A central value of what may be termed Experience Design is its aspiration to shift focus from the logic of singular design fields to the interrelations and interactions that take place in situations where people are simultaneously engaged with multiple designs. Experience Design can allow...... researchers and practitioners to travel – making it possible to follow experiences as they are enacted across and between places, modes of transportation, mobile mediation and assemblages of things. Drawing on the nomadic metaphysics of philosopher Michel Serres, the journeying, shifting and propagating...... qualities of experience are highlighted as part of a suggestion that design may indeed relate as much to metaphysics as to mechanics, materials science, and the psychology of the consumer and user. An Experience Design is sketched out as the choreography of temporary and shifting engagements across...

  17. The Experience City

    DEFF Research Database (Denmark)

    Marling, Gitte; Jensen, Ole B.; Kiib, Hans

    2012-01-01

    This article take its point of departure in the pressure of the experience economy on European cities - a pressure which in recent years has found its expression in a number of comprehensive transformations of the physical and architectural environments, and new eventscapes related to fun...... and cultural experience are emerging. The physical, cultural and democratic consequences of this development are discussed in the article, as well as the problems and the new opportunities in the ‘Experience city’. It focuses on the design of the ‘Danish Experience City’ with a special emphasis on hybrid...... cultural projects and on performative urban spaces. ‘Hybrid cultural projects’ are characterised by a conscious fusion between urban transformation and new knowledge centres, cultural institutions and experience environments. ‘Performative urban spaces” are characterised by stages for performance...

  18. Experience Communication and Aesthetics

    DEFF Research Database (Denmark)

    Thorlacius, Lisbeth

    to user aspect (web 2.0), the personal engagement or the community spirit. This increasing demand of experiences reflects the postmodern cultural trends where rules for how to think and behave no longer exist. This results in individualism, where the identity of the human being has changed from something...... which was a given to something which each human being has to manage individually. As a consequence the human being experiences an increasing sense of insecurity and restless seeking after identity and recognition, which is reflected in an increasing demand of among other things self......In this article the term "experience communication" will be introduced and discussed. It will be illustrated how different concepts of aesthetical experiences are an integrated part of experience communication and how these concepts are produced within the industries of consumerism, branding...

  19. The Experience City

    DEFF Research Database (Denmark)

    Marling, Gitte; Jensen, Ole B.; Kiib, Hans

    2009-01-01

      The article take its point of departure in the pressure of the experience economy on European cities - a pressure which in recent years has found its expression in a number of comprehensive transformations of the physical and architectural environments, and new eventscapes related to fun...... and cultural experience are emerging. In the discussion of the transformation into the ‘experience economy' relevant to cities and urban areas we rarely find an analysis of the physical and spatial implications of this transformation. However, the physical, cultural and democratic consequences...... of this development are discussed in the paper, as well as the problems and the new opportunities with which the ‘Experience city' is faced. The article focus on the design of the Danish Experience City with special emphasis on hybrid cultural projects and on performative urban spaces. It present the first findings...

  20. Cryogenics for LHC experiments

    CERN Multimedia

    2001-01-01

    Cryogenic systems will be used by LHC experiments to maximize their performance. Institutes around the world are collaborating with CERN in the construction of these very low temperature systems. The cryogenic test facility in hall 180 for ATLAS magnets. High Energy Physics experiments have frequently adopted cryogenic versions of their apparatus to achieve optimal performance, and those for the LHC will be no exception. The two largest experiments for CERN's new flagship accelerator, ATLAS and CMS, will both use large superconducting magnets operated at 4.5 Kelvin - almost 270 degrees below the freezing point of water. ATLAS also includes calorimeters filled with liquid argon at 87 Kelvin. For the magnets, the choice of a cryogenic version was dictated by a combination economy and transparency to emerging particles. For the calorimeters, liquid argon was selected as the fluid best suited to the experiment's physics requirements. High Energy Physics experiments are the result of worldwide collaborations and...

  1. USML-1 Glovebox experiments

    Science.gov (United States)

    Naumann, Robert J.

    1995-01-01

    This report covers the development of and results from three experiments that were flown in the Materials Science Glovebox on USML-1: Marangoni convection in Closed Containers (MCCC), Double Float Zone (DFZ), and Fiber Pulling in Microgravity (FPM). The Glovebox provided a convenient, low cost method for doing simple 'try and see' experiments that could test new concepts or elucidate microgravity phenomena. Since the Glovebox provided essentially one (or possibly two levels of confinement, many of the stringent verification and test requirements on the experiment apparatus could be relaxed and a streamlined test and verification plan for flight qualification could be implemented. Furthermore, the experiments were contained in their own carrying cases whose external configurations could be identified early in the integration sequence for stowage considerations while delivery of the actual experiment apparatus could be postponed until only a few months before flight. This minimized the time fluids must be contained and reduced the possibility of corrosive reactions that could ruin the experiment. In many respects, this exercise was as much about developing a simpler, cheaper way of doing crew-assisted science as it was about the actual scientific accomplishments of the individual experiments. The Marangoni Convection in Closed Containers experiment was designed to study the effects of a void space in a simulated Bridgman crystal growth configuration and to determine if surface tension driven convective flows that may result from thermal gradients along any free surfaces could affect the solidification process. The Fiber Pulling in Microgravity experiment sought to separate the role of gravity drainage from capillarity effects in the break-up of slender cylindrical liquid columns. The Stability of a Double Float Zone experiment explored the feasibility of a quasi-containerless process in which a solidifying material is suspended by two liquid bridges of its own melt.

  2. Simulation - modeling - experiment; Simulation - modelisation - experience

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2004-07-01

    After two workshops held in 2001 on the same topics, and in order to make a status of the advances in the domain of simulation and measurements, the main goals proposed for this workshop are: the presentation of the state-of-the-art of tools, methods and experiments in the domains of interest of the Gedepeon research group, the exchange of information about the possibilities of use of computer codes and facilities, about the understanding of physical and chemical phenomena, and about development and experiment needs. This document gathers 18 presentations (slides) among the 19 given at this workshop and dealing with: the deterministic and stochastic codes in reactor physics (Rimpault G.); MURE: an evolution code coupled with MCNP (Meplan O.); neutronic calculation of future reactors at EdF (Lecarpentier D.); advance status of the MCNP/TRIO-U neutronic/thermal-hydraulics coupling (Nuttin A.); the FLICA4/TRIPOLI4 thermal-hydraulics/neutronics coupling (Aniel S.); methods of disturbances and sensitivity analysis of nuclear data in reactor physics, application to VENUS-2 experimental reactor (Bidaud A.); modeling for the reliability improvement of an ADS accelerator (Biarotte J.L.); residual gas compensation of the space charge of intense beams (Ben Ismail A.); experimental determination and numerical modeling of phase equilibrium diagrams of interest in nuclear applications (Gachon J.C.); modeling of irradiation effects (Barbu A.); elastic limit and irradiation damage in Fe-Cr alloys: simulation and experiment (Pontikis V.); experimental measurements of spallation residues, comparison with Monte-Carlo simulation codes (Fallot M.); the spallation target-reactor coupling (Rimpault G.); tools and data (Grouiller J.P.); models in high energy transport codes: status and perspective (Leray S.); other ways of investigation for spallation (Audoin L.); neutrons and light particles production at intermediate energies (20-200 MeV) with iron, lead and uranium targets (Le Colley F

  3. Understanding customer experience.

    Science.gov (United States)

    Meyer, Christopher; Schwager, Andre

    2007-02-01

    Anyone who has signed up for cell phone service, attempted to claim a rebate, or navigated a call center has probably suffered from a company's apparent indifference to what should be its first concern: the customer experiences that culminate in either satisfaction or disappointment and defection. Customer experience is the subjective response customers have to direct or indirect contact with a company. It encompasses every aspect of an offering: customer care, advertising, packaging, features, ease of use, reliability. Customer experience is shaped by customers' expectations, which largely reflect previous experiences. Few CEOs would argue against the significance of customer experience or against measuring and analyzing it. But many don't appreciate how those activities differ from CRM or just how illuminating the data can be. For instance, the majority of the companies in a recent survey believed they have been providing "superior" experiences to customers, but most customers disagreed. The authors describe a customer experience management (CEM) process that involves three kinds of monitoring: past patterns (evaluating completed transactions), present patterns (tracking current relationships), and potential patterns (conducting inquiries in the hope of unveiling future opportunities). Data are collected at or about touch points through such methods as surveys, interviews, focus groups, and online forums. Companies need to involve every function in the effort, not just a single customer-facing group. The authors go on to illustrate how a cross-functional CEM system is created. With such a system, companies can discover which customers are prospects for growth and which require immediate intervention.

  4. The three eggs experiment

    Science.gov (United States)

    Şahin Bülbül, Mustafa

    2016-01-01

    The three eggs experiment concerns 37 pre-service science teachers’ predictions about the impact shapes of three uncooked eggs dropped from different heights. This experiment looks at energy transformation from potential to kinetic energy, where the smaller parts of the egg shell spread far from the center of the impact. This experience encouraged the pre-service science teachers to use their familiar models, such as a fried egg, omelet, puddle, dropping or explosions, to explain their predictions. These models from everyday life presented can be used as a tool to explain unfamiliar phenomena.

  5. Nightshade Prototype Experiments (Silverleaf)

    Energy Technology Data Exchange (ETDEWEB)

    Danielson, Jeremy [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Bauer, Amy L. [Los Alamos National Lab. (LANL), Los Alamos, NM (United States)

    2016-12-23

    The Red Sage campaign is a series of subcritical dynamic plutonium experiments designed to measure ejecta. Nightshade, the first experiments in Red Sage scheduled for fiscal year 2019, will measure the amount of ejecta emission into vacuum from a double-­shocked plutonium surface. To address the major technical risks in Nightshade, a Level 2 milestone was developed for fiscal year 2016. Silverleaf, a series of four experiments, was executed at the Los Alamos National Laboratory in July and August 2016 to demonstrate a prototype of the Nightshade package and to satisfy this Level 2 milestone. This report is documentation that Red Sage Level 2 milestone requirements were successfully met.

  6. Game user experience evaluation

    CERN Document Server

    Bernhaupt, Regina

    2015-01-01

    Evaluating interactive systems for their user experience (UX) is a standard approach in industry and research today. This book explores the areas of game design and development and Human Computer Interaction (HCI) as ways to understand the various contributing aspects of the overall gaming experience. Fully updated, extended and revised this book is based upon the original publication Evaluating User Experience in Games, and provides updated methods and approaches ranging from user- orientated methods to game specific approaches. New and emerging methods and areas explored include physiologi

  7. Making the Experience City

    DEFF Research Database (Denmark)

    Jensen, Ole B.

    2009-01-01

    makes an investigation into the complex relationship between the words and policies of the ‘Experience Economy' and the actual urban transformations made in cities with reference to these changes. The paper discusses the cases researched in relation to the state, market, civil society framework as well......This paper describes the latest research into cultural planning and architectural branding in Denmark based on the ‘Experience City' research project located at Aalborg University. The paper explores the implication of the turn towards culture and experience in the contemporary Danish city. It thus...

  8. Hydrothermal organic synthesis experiments

    Science.gov (United States)

    Shock, Everett L.

    1992-01-01

    Ways in which heat is useful in organic synthesis experiments are described, and experiments on the hydrothermal destruction and synthesis of organic compounds are discussed. It is pointed out that, if heat can overcome kinetic barriers to the formation of metastable states from reduced or oxidized starting materials, abiotic synthesis under hydrothermal conditions is a distinct possibility. However, carefully controlled experiments which replicate the descriptive variables of natural hydrothermal systems have not yet been conducted with the aim of testing the hypothesis of hydrothermal organic systems.

  9. Experimenting for resilience

    DEFF Research Database (Denmark)

    Hagedorn-Rasmussen, Peter; Dupret, Katia

    Focusing on how an experimental approach to organizing may pave the way for organizational resilience, we explore opportunities and barriers of experimental organizing by following a concrete social experiment in civil society and discuss its adaptability in traditional organizations. The social...... experiment is called Civic Desire. The founders explicitly call for new ways of organizing that can develop social sustainability. We discuss how these experiments may create platforms of new unforeseen goals that organizations may choose to follow. In conclusion we argue for organizational resilience...

  10. Network simulation experiments manual

    CERN Document Server

    Aboelela, Emad

    2011-01-01

    Network Simulation Experiments Manual, Third Edition, contains simulation-based experiments to help students and professionals learn about key concepts in computer networking. The simulation approach provides a virtual environment for a wide range of desirable features, such as modeling a network based on specified criteria and analyzing its performance under different scenarios. The experiments include the basics of using OPNET IT Guru Academic Edition; operation of the Ethernet network; partitioning of a physical network into separate logical networks using virtual local area networks (V

  11. THERMAL DISTRIBUTION SYSTEM EXPERIMENT

    Energy Technology Data Exchange (ETDEWEB)

    KRAJEWSKI,R.F.; ANDREWS,J.W.; WEI,G.

    1999-09-01

    A laboratory experiment has been conducted which tests for the effects of distribution system purging on system Delivery Effectiveness (DE) as defined in ASHRAE 152P. The experiment is described in its configuration, instrumentation, and data acquisition system. Data gathered in the experiment is given and discussed. The results show that purging of the distribution system alone does not offer any improvement of the system DE. Additional supporting tests were conducted regarding experimental simulations of buffer zones and bare pipe and are also discussed.

  12. Phenomenological Account of Religious Experience

    Directory of Open Access Journals (Sweden)

    Donny Gahral Adian

    2011-08-01

    Full Text Available History of philosophy is built upon rigid discrimination between various human experiences. Human experiences are divided mainly into two major experiences: Perceptual and intelectual. Perceptual experience is deined by empiricism as an aposteriori experience of empirical sensations. Meanwhile, rationalism claims that the only acceptable experience is apriori experience of intelectual object (natural laws, mathematical equations and logical operations. There is no other experience outside those two philosophical account of experiences. All other experiences must be subsumed either within perceptual or intelectual experience.

  13. The Experience City

    DEFF Research Database (Denmark)

    Marling, Gitte; Jensen, Ole B.; Kiib, Hans

    2009-01-01

    of a research project  mapping the different approaches to urban transformation and experience design, to the level of strategic thinking and planning, and to the content of the programs implemented. Special emphasis is put on on projects combining experience, leisure and learning, and on projects which have...... concepts and framings that will guide the understanding and the analysis of the experience city. In section three we focus on the design of the Danish experience city and present the first findings of the research project The projects are categorised according to their content, structure and urban...... localisation. In particular the cases are represented in relation to their strategic and urban planning importance, their social and cultural content and their architectural representation and the programmes they contain. The article ends with a short discussion of some  of these preliminary findings as well...

  14. Conceptualising the audiobook experience

    Directory of Open Access Journals (Sweden)

    Iben Have

    2012-12-01

    Full Text Available In this article we wish to introduce and discuss a theoretical framework for a possible conceptualisation of the differences between reading a printed book and listening to an audiobook. We tend to introduce similarities and differences between reading with the eyes and reading with the ears, implying that we should not discuss the audiobook experience as a remediation of the printed book experience only, but as an entirely different experience that could be conceptualised in continuation of mobile listening practises. As a methodological strategy we will emphasise the differences between the literary practices, reading with the eyes and reading with the ears. These different perspectives on reading are used to accentuate the distinct experiences, and future thorough analyses in continuation of this framework would appear much more complex and connected than in the present article.

  15. Cooperative Prototyping Experiments

    DEFF Research Database (Denmark)

    Bødker, Susanne; Grønbæk, Kaj

    1989-01-01

    This paper describes experiments with a design technique that we denote cooperative prototyping. The experiments consider design of a patient case record system for municipal dental clinics in which we used HyperCard, an off the shelf programming environment for the Macintosh. In the ecperiments we...... tried to achieve a fluent work-like evaluation of prototypes where users envisioned future work with a computer tool, at the same time as we made on-line modifications of prototypes in cooperation with the users when breakdown occur in their work-like evaluation. The experiments showed...... these experiences we discuss problems in the process, requirements for design tools, and issues involved in getting going with cooperative prototyping with active user involvement....

  16. The world's biggest experiment

    CERN Document Server

    Gregson, Liz

    2008-01-01

    According to CERN, our understanding of the Universe is about the change. Meet the Imperial alumni and staff who are involved in CERN's Large Hadron Collider, the world's biggest experiment. (3 pages)

  17. Lidar calibration experiments

    DEFF Research Database (Denmark)

    Ejsing Jørgensen, Hans; Mikkelsen, T.; Streicher, J.

    1997-01-01

    A series of atmospheric aerosol diffusion experiments combined with lidar detection was conducted to evaluate and calibrate an existing retrieval algorithm for aerosol backscatter lidar systems. The calibration experiments made use of two (almost) identical mini-lidar systems for aerosol cloud...... detection to test the reproducibility and uncertainty of lidars. Lidar data were obtained from both single-ended and double-ended Lidar configurations. A backstop was introduced in one of the experiments and a new method was developed where information obtained from the backstop can be used in the inversion...... algorithm. Independent in-situ aerosol plume concentrations were obtained from a simultaneous tracer gas experiment with SF6, and comparisons with the two lidars were made. The study shows that the reproducibility of the lidars is within 15%, including measurements from both sides of a plume...

  18. User Experience Dimensions

    DEFF Research Database (Denmark)

    Lykke, Marianne; Jantzen, Christian

    2016-01-01

    The present study develops a set of 10 dimensions based on a systematic understanding of the concept of experience as a holistic psychological. Seven of these are derived from a psychological conception of what experiencing and experiences are. Three supplementary dimensions spring from...... the observation that experiences apparently have become especially valuable phenomena in Western societies. The 10 dimensions are tried out in a field study at the Center for Art and Media (ZKM) in Germany with the purpose to study their applicability in the evaluation of interactive sound archives. 29 walk......-alongs were carried out with 58 museums visitors. Our analysis showed that it was possible to identify the 10 experience dimensions in the study material. Some dimensions were expressed more frequently than others. The distribution of expressed dimensions and the content of the user comments provided a clear...

  19. Accessibility and sensory experiences

    DEFF Research Database (Denmark)

    Ryhl, Camilla

    2010-01-01

    This article introduces a new design concept; sensory accessibility. While acknowledging the importance of sensory experiences in architectural quality, as well as the importance of accommodating user needs the concept combines three equally important factors; architecture, the senses and accessi......This article introduces a new design concept; sensory accessibility. While acknowledging the importance of sensory experiences in architectural quality, as well as the importance of accommodating user needs the concept combines three equally important factors; architecture, the senses...... and accessibility. Sensory accessibility accommodates aspects of a sensory disability and describes architectural design requirements needed to ensure access to architectural experiences. In the context of architecture accessibility has become a design concept of its own. It is generally described as ensuring...... physical access to the built environment by accommodating physical disabilities. While the existing concept of accessibility ensures the physical access of everyone to a given space, sensory accessibility ensures the choice of everyone to stay and be able to participate and experience....

  20. Foraging Experiences with Children

    Science.gov (United States)

    Russell, Helen Ross

    1976-01-01

    Provided are foraging experiences and wild foods information for utilization in the urban school curriculum. Food uses are detailed for roses, dandelions, wild onions, acorns, cattails, violets and mints. (BT)

  1. [Mechanical staplers. Personal experience].

    Science.gov (United States)

    Carzaniga, P L; Gatti, A; Russo, F; Sforza, M; Vertemati, G

    1992-12-01

    The Authors briefly summarise their experience of using various types of mechanical staplers in abdominal and thoracic surgery with excellent results both in general and in terms of reducing operating times.

  2. Experience and Its Generation

    Institute of Scientific and Technical Information of China (English)

    Chen Youqing

    2006-01-01

    Experience iS an activity that arouses emotions and generates meanings based on vivid sensation and profound compreh ension.It iS emotional,meaningful,and personal,playing a key role in the course of forming and developing one'S qualities.The psychological process of experience generation consists of such links as sensing things,arousing emotions,promoting comprehension and association,generating insights and meanings,and deepening emotional responses.Undergoing things personally by means of direct sensation,taking part in activities,and living life are the most important preconditions of experience generation.Emotional influence,situational edification,and arts edification ale extemal factors that induce experience generation.

  3. Experiments with Helmholtz Resonators.

    Science.gov (United States)

    Greenslade, Thomas B., Jr.

    1996-01-01

    Presents experiments that use Helmholtz resonators and have been designed for a sophomore-level course in oscillations and waves. Discusses the theory of the Helmholtz resonator and resonance curves. (JRH)

  4. Experiments in Animal Behavior

    Science.gov (United States)

    Polt, James M.

    1971-01-01

    Describes experiments in conditioning, sensory processes, social behavior, imprinting, innate preferences for color and form, and discrimination learning suitable for secondary school students. Mealworms, crickets, and chicks are used as subjects. (AL)

  5. Experiment-o-mania

    Science.gov (United States)

    Drndarski, Marina

    2015-04-01

    Every 21st century student is expected to develop science literacy skills. As this is not part of Serbian national curriculum yet, we decided to introduce it with this project. Experiment-o-mania provides students to experience science in different and exciting way. It makes opportunity for personalized learning offering space and time to ask (why, where, how, what if) and to try. Therefore, we empower young people with skills of experimenting, and they love science back. They ask questions, make hypothesis, make problems and solve them, make mistakes, discuss about the results. Subsequently this raises the students' interest for school curriculum. This vision of science teaching is associated with inquiry-based learning. Experiment-o-mania is the unique and recognizable teaching methodology for the elementary school Drinka Pavlović, Belgrade, Serbia. Experiment-o-mania implies activities throughout the school year. They are held on extra class sessions, through science experiments, science projects or preparations for School's Days of science. Students learn to ask questions, make observations, classify data, communicate ideas, conduct experiments, analyse results and make conclusions. All science teachers participate in designing activities and experiments for students in Experiment-o-mania teaching method. But they are not alone. Teacher of fine arts, English teachers and others also take part. Students have their representatives in this team, too. This is a good way to blend knowledge among different school subject and popularize science in general. All the experiments are age appropriate and related to real life situations, local community, society and the world. We explore Fibonacci's arrays, saving energy, solar power, climate change, environmental problems, pollution, daily life situations in the country or worldwide. We introduce great scientists as Nikola Tesla, Milutin Milanković and sir Isaac Newton. We celebrate all relevant international days, weeks

  6. Illegal Entrepreneurship Experience

    OpenAIRE

    2004-01-01

    Existing studies show a positive relationship between entrepreneurs' business performance and their conventional human capital as measured by previous business experience and formal education. In this paper, we explore whether illegal entrepreneurship experience (IEE), an unconventional form of human capital, is related to the performance and motivation of entrepreneurs operating legal businesses in a transition context. Using regression techniques on a sample of 399 private business owners i...

  7. Sculpting the Illness Experience

    Directory of Open Access Journals (Sweden)

    Molly Bathje MS, OTR/L

    2014-10-01

    Full Text Available Otto Kamensek provided the cover art for the Fall 2014 issue of the Open Journal of Occupational Therapy. “Glimmer of Hope” is part of Otto’s collection “Shard’s, Bone Deep,” which includes hand-built ceramic sculptures that portray his experiences with a lifelong chronic illness. Engaging in ceramic sculpture helps him process the experiences associated with Juvenile Rheumatoid Arthritis and provides a means to support others experiencing chronic illness.

  8. The CLOUD experiment

    CERN Multimedia

    Maximilien Brice

    2006-01-01

    The Cosmics Leaving Outdoor Droplets (CLOUD) experiment as shown by Jasper Kirkby (spokesperson). Kirkby shows a sketch to illustrate the possible link between galactic cosmic rays and cloud formations. The CLOUD experiment uses beams from the PS accelerator at CERN to simulate the effect of cosmic rays on cloud formations in the Earth's atmosphere. It is thought that cosmic ray intensity is linked to the amount of low cloud cover due to the formation of aerosols, which induce condensation.

  9. Digital Heritage Experiences

    DEFF Research Database (Denmark)

    Munar, Ana Maria; Ooi, Can-Seng

    The evolution of the Web and the expansion of social media are transforming our heritage experiences. Social media offer an innovative element to personal travel reflections by providing digital global platforms on which tourists can create and publish their travel stories. Social media transform...... the traditional creative processes, distribution mechanisms and consumption patterns of these experiences. This study provides insight on how personal heritage moments are constructed, digitalized and shared. The methodological perspective adopted draws on a constructivist epistemology (Markham 2004...

  10. Future Reactor Experiments

    OpenAIRE

    He, Miao

    2013-01-01

    The measurement of the neutrino mixing angle $\\theta_{13}$ opens a gateway for the next generation experiments to measure the neutrino mass hierarchy and the leptonic CP-violating phase. Future reactor experiments will focus on mass hierarchy determination and the precision measurement of mixing parameters. Mass hierarchy can be determined from the disappearance of reactor electron antineutrinos based on the interference effect of two separated oscillation modes. Relative and absolute measure...

  11. Reactor antineutrino experiments

    OpenAIRE

    Lu, Haoqi

    2014-01-01

    Neutrinos are elementary particles in the standard model of particle physics. There are 3 flavors of neutrinos that oscillate among themselves. Their oscillation can be described by a 3$\\times$3 unitary matrix, containing three mixing angles $\\theta_{12}$, $\\theta_{23}$, $\\theta_{13}$, and one CP phase. Both $\\theta_{12}$ and $\\theta_{23}$ are known from previous experiments. $\\theta_{13}$ was unknown just two years ago. The Daya Bay experiment gave the first definitive non-zero value in 2012...

  12. Surface electrical properties experiment

    Science.gov (United States)

    Simmons, Gene; Strangway, David; Annan, Peter; Baker, Richard G.; Bannister, Lawrence; Brown, Raymon; Cooper, William; Cubley, Dean; deBettencourt, Joseph; England, Anthony W.; Groener, John; Kong, Jin-Au; LaTorraca, Gerald; Meyer, James; Nanda, Ved; Redman, David; Rossiter, James; Tsang, Leung; Urner, Joseph; Watts, Raymond

    1973-01-01

    The surface electrical properties (SEP) experiment was used to explore the subsurface material of the Apollo 17 landing site by means of electromagnetic radiation. The experiment was designed to detect electrical layering, discrete scattering bodies, and the possible presence of water. From the analysis of the data, it was expected that values of the electrical properties (dielectric constant and loss tangent) of lunar material in situ would be obtained.

  13. Mechatronics with experiments

    CERN Document Server

    Cetinkunt, Sabri

    2014-01-01

    Mechatronics with Experiments, Second Edition comprehensively covers the fundamental scientific principles and technologies that are used in the design of modern computer-controlled machines and processes. It provides all of the technical background (covering mechanical, aerospace, chemical, electrical, and computer engineering) needed for designing an automated machine or process. The new edition of this textbook has been updated to include a number of experiments involving electronic circuit design and microcontroller programming and includes real time software development using MATLAB® and

  14. The OPERA Experiment

    CERN Document Server

    Iovane, G

    1998-01-01

    OPERA(Oscillation Project with Emulsion-tRacking Apparatus) is a new detector concept, iron(lead)-emulsion for a long-baseline neutrino oscillation experiment. This experiment would perform an appearance search for nu_mu-nu_tau oscillation in the parameter region indicated by the atmospheric neutrino anomaly. OPERA can run at the Gran Sasso Laboratory in the proposed NGS (Neutrino to Gran Sasso) Beam from CERN.

  15. Hamlet and psychoanalytic experience.

    Science.gov (United States)

    Schwaber, Paul

    2007-01-01

    Hamlet draws us into its rendered world, enabling us to experience it with depth, awareness, and resonance, in a mode we recognize as aesthetic. By way of Shakespeare's play--primarily the first act--and a detailed case study, aesthetic and psychoanalytic experience are compared, to suggest that, for our own analytic discourse, we revalue Freud's unease that his case studies read like short stories.

  16. The LHCb experiment

    CERN Document Server

    Nakada, Tatsuya

    2000-01-01

    The LHCb experiment is designed to fully exploit the large number of b hadrons expected at the LHC energy and luminosity. The experiment is equipped with particle identification devices and can efficiently trigger events with different B-meson final states, allowing systematic studies of CP violation and other rare phenomena in the b hadron system with a high precision which could reveal physics beyond the Standard Model.

  17. The MAJORANA Experiment

    Energy Technology Data Exchange (ETDEWEB)

    Aguayo Navarrete, Estanislao; Avignone, F. T.; Back, Henning O.; Barabash, Alexander S.; Bergevin, M.; Bertrand, F.; Boswell, M.; Brudanin, V.; Busch, Matthew; Chan, Yuen-Dat; Christofferson, Cabot-Ann; Collar, J. I.; Combs, Dustin C.; Cooper, R. J.; Detwiler, Jason A.; Doe, Peter J.; Efremenko, Yuri; Egorov, Viatcheslav; Ejiri, H.; Elliott, Steven R.; Esterline, James H.; Fast, James E.; Fields, N.; Finnerty, P.; Fraenkle, Florian; Gehman, Victor M.; Giovanetti, G. K.; Green, Matthew P.; Guiseppe, Vincente; Gusey, K.; Hallin, A. L.; Hazama, R.; Henning, R.; Hime, Andrew; Hoppe, Eric W.; Horton, Mark; Howard, Stanley; Howe, M. A.; Johnson, R. A.; Keeter, K.; Keillor, Martin E.; Keller, C.; Kephart, Jeremy D.; Kidd, Mary; Knecht, A.; Kochetov, Oleg; Konovalov, S.; Kouzes, Richard T.; LaFerriere, Brian D.; LaRoque, B. H.; Leon, Jonathan D.; Leviner, L.; Loach, J. C.; MacMullin, S.; Marino, Michael G.; Martin, R. D.; Mei, Dong-Ming; Merriman, Jason H.; Miller, M. L.; Mizouni, Leila; Nomachi, Masaharu; Orrell, John L.; Overman, Nicole R.; Phillips, D.; Poon, Alan; Perumpilly, Gopakumar; Prior, Gersende; Radford, D. C.; Rielage, Keith; Robertson, R. G. H.; Ronquest, M. C.; Schubert, Alexis G.; Shima, T.; Shirchenko, M.; Snavely, Kyle J.; Sobolev, V.; Steele, David; Strain, J.; Thomas, K.; Timkin, V.; Tornow, W.; Vanyushin, I.; Varner, R. L.; Vetter, Kai; Vorren, Kris R.; Wilkerson, John; Wolfe, B. A.; Yakushev, E.; Young, A.; Yu, Chang-Hong; Yumatov, Vladimir; Zhang, C.

    2011-10-01

    The Majorana collaboration is actively pursuing research and development aimed at a tonne-scale {sup 76}Ge neutrinoless double-beta decay ({beta}{beta}(0{nu})-decay) experiment. The current, primary focus is the construction of the Majorana Demonstrator experiment, an R and D effort that will field approximately 40 kg of germanium detectors with mixed enrichment levels. This article provides a status update on the construction of the Demonstrator.

  18. Prolonged labour : women's experiences

    OpenAIRE

    Nystedt, Astrid

    2005-01-01

    Aim: The overall aim of this thesis was to illuminate, describe, and promote understanding of women’s experiences of prolonged labour. The thesis compromises four studies. Methods: Paper I describes a case-referent study that recruited women (n = 255) giving singleton live birth to their first child by spontaneous labour after more than 37 completed weeks’ pregnancy. Participants completed a questionnaire that investigated childbirth experiences, previous family relationships, and childhood e...

  19. Radiochemical solar neutrino experiments

    CERN Document Server

    Gavrin, V N

    2011-01-01

    Radiochemical experiments have been crucial to solar neutrino research. Even today, they provide the only direct measurement of the rate of the proton-proton fusion reaction, p + p --> d + e^+ + nu_e, which generates most of the Sun's energy. We first give a little history of radiochemical solar neutrino experiments with emphasis on the gallium experiment SAGE -- the only currently operating detector of this type. The combined result of all data from the Ga experiments is a capture rate of 67.6 +/- 3.7 SNU. For comparison to theory, we use the calculated flux at the Sun from a standard solar model, take into account neutrino propagation from the Sun to the Earth and the results of neutrino source experiments with Ga, and obtain 67.3 ^{+3.9}_{-3.5} SNU. Using the data from all solar neutrino experiments we calculate an electron neutrino pp flux at the earth of (3.41 ^{+0.76}_{-0.77}) x 10^{10}/(cm^2-s), which agrees well with the prediction from a detailed solar model of (3.30 ^{+0.13} _{-0.14}) x 10^{10}/(cm^...

  20. Reconstructing Experiences through Sketching

    CERN Document Server

    Karapanos, Evangelos; Hassenzahl, Marc

    2009-01-01

    This paper presents iScale, a survey tool that aims at eliciting users' experiences with a product from memory. iScale employs sketching in imposing a process in the reconstruction of one's experiences. Two versions of iScale, the Constructive and the Value-Account iScale, were motivated by two distinct theories on how people reconstruct emotional experiences from memory. These two versions were tested in two separate studies. Study 1 aimed at providing qualitative insight into the use of iScale and compared its performance to that of free-hand sketching. Study 2 compared the two iScale versions to a control condition: that of reporting one's experiences without employing any form of sketching. Significant differences between iScale and the "no-sketching" tool were found. Overall, iScale resulted in a) an increase in the number of experience reports that subjects provided, b) an increase in the amount of contextual information for the reported experiences, and c) an increase in subjects' accuracy in recalling...

  1. Experiments in computing: a survey.

    Science.gov (United States)

    Tedre, Matti; Moisseinen, Nella

    2014-01-01

    Experiments play a central role in science. The role of experiments in computing is, however, unclear. Questions about the relevance of experiments in computing attracted little attention until the 1980s. As the discipline then saw a push towards experimental computer science, a variety of technically, theoretically, and empirically oriented views on experiments emerged. As a consequence of those debates, today's computing fields use experiments and experiment terminology in a variety of ways. This paper analyzes experimentation debates in computing. It presents five ways in which debaters have conceptualized experiments in computing: feasibility experiment, trial experiment, field experiment, comparison experiment, and controlled experiment. This paper has three aims: to clarify experiment terminology in computing; to contribute to disciplinary self-understanding of computing; and, due to computing's centrality in other fields, to promote understanding of experiments in modern science in general.

  2. The POLARBEAR Experiment

    CERN Document Server

    Kermish, Z; Anthony, A; Arnold, K; Arnold, K; Barron, D; Boettger, D; Borrill, J; Chapman, S; Chinone, Y; Dobbs, M A; Errard, J; Fabbian, G; Flanigan, D; Fuller, G; Ghribi, A; Grainger, W; Halverson, N; Hasegawa, M; Hattori, K; Hazumi, M; Holzapfel, W L; Howard, J; Hyland, P; Jaffe, A; Keating, B; Kisner, T; Lee, A T; Jeune, M Le; Linder, E; Lungu, M; Matsuda, F; Matsumura, T; Meng, X; Miller, N J; Morii, H; Moyerman, S; Myers, M J; Nishino, H; Paar, H; Quealy, E; Reichardt, C L; Richards, P L; Ross, C; Shimizu, A; Shimon, M; Shimmin, C; Sholl, M; Siritanasak, P; Spieler, H; Stebor, N; Steinbach, B; Stompor, R; Suzuki, A; Tomaru, T; Tucker, C; Zahn, O

    2012-01-01

    We present the design and characterization of the POLARBEAR experiment. POLARBEAR will measure the polarization of the cosmic microwave background (CMB) on angular scales ranging from the experiment's 3.5 arcminute beam size to several degrees. The experiment utilizes a unique focal plane of 1,274 antenna-coupled, polarization sensitive TES bolometers cooled to 250 milliKelvin. Employing this focal plane along with stringent control over systematic errors, POLARBEAR has the sensitivity to detect the expected small scale B-mode signal due to gravitational lensing and search for the large scale B-mode signal from inflationary gravitational waves. POLARBEAR was assembled for an engineering run in the Inyo Mountains of California in 2010 and was deployed in late 2011 to the Atacama Desert in Chile. An overview of the instrument is presented along with characterization results from observations in Chile.

  3. Qualitative experiments in psychology

    DEFF Research Database (Denmark)

    Wagoner, Brady

    2015-01-01

    In this article, I explore the meaning of experiments in early twentieth century psychology, focusing on the qualitative experimental methodology of psychologist Frederic BARTLETT. I begin by contextualizing BARTLETT's experiments within the continental research tradition of his time, which...... was in a state of transition from a focus on elements (the concern of psychophysics) to a focus on wholes (the concern of Gestalt psychology). The defining feature of BARTLETT's early experiments is his holistic treatment of human responses, in which the basic unit of analysis is the active person relating...... to some material within the constraints of a social and material context. This manifests itself in a number of methodological principles that contrast with contemporary understandings of experimentation in psychology. The contrast is further explored by reviewing the history of "replications...

  4. Learning From Experience

    DEFF Research Database (Denmark)

    Visholm, Steen; Beck, Ulla Charlotte

    2014-01-01

    University and NAPSO2). Seen from the horizon of their experience some of the basic concepts in the theories about GRC need clarifying, revision, and development. The GRC is a part of the learning from experience movement and as a consequence it stresses the underlying basis: learning is personal so everyone......In this paper the learning concept of group relation's conferences are discussed. The authors have worked with group relations conferences (GRC) in different contexts for many years-mainly as a part of educational programmes for managers and consultants (OPU at IGA Copenhagen, MPO at Roskilde...... decides for themselves what makes sense and what does not. This principle sometimes works as a defence against a closer examination of the two questions: do GRCs provide relevant experiences to learn from, and what is it you learn or can expect to learn at a GRC. Here the learning concept of the GRCs...

  5. The interprofessional learning experience

    DEFF Research Database (Denmark)

    Jakobsen, Flemming; Mørcke, Anne Mette; Hansen, Torben Bæk

    2017-01-01

    we ensure that this shift maximises learning. The purpose of this article is to understand the authentic learning experience in an interprofessional outpatient clinic setting. We performed an exploratory case study with interviews of four nursing students, 13 medical students, and six staff members...... who worked in an interprofessional outpatient orthopaedic clinic from March 2015 to January 2016. The interviews were transcribed and analysed using systematic text condensation. The students' self-reported learning experience in this outpatient clinic was characterised by direct patient contact...... and by authentic, interprofessional, task-based learning, and a preference for indirect supervision when conducting uncomplicated patient consultations. The supervisors intended to create this interprofessional outpatient clinic experience by having a clear teaching approach based on adult learning principles...

  6. Experience and Sustainable Consumption

    DEFF Research Database (Denmark)

    Rasmussen, Tove Arendt

    consumption may re-enchant ordinary consumption and thereby even become a part of marketing and the experience economy. New layers of meaning are at stake and altruistic motives come into play; doing something good for someone or something, aside from oneself, is a very strong trigger of positive emotions......Experience understood as experience-based consumption is by now fairly absent from the research agenda of the different theories on sustainable consumption. On the basis of Colin Campbell’s notion of romantic ethics and emotional sentimentality in modern hedonism, I claim that sustainable....... Very often, however, the actual purchase does not live up to the demands of doing good in the sustainable consumption chain, and the individual might end up with a guilty conscience, which again is a possible trigger for lingering in a sentimental mode of guilt. Emotions of sentimentality may actually...

  7. Experience and Sustainable Consumption

    DEFF Research Database (Denmark)

    Rasmussen, Tove Arendt

    2014-01-01

    consumption may re-enchant ordinary consumption and thereby even become a part of marketing and the experience economy. New layers of meaning are at stake and altruistic motives come into play; doing something good for someone or something, aside from oneself, is a very strong trigger of positive emotions......Experience understood as experience-based consumption is by now fairly absent from the research agenda of the different theories on sustainable consumption. On the basis of Colin Campbell’s notion of romantic ethics and emotional sentimentality in modern hedonism, I claim that sustainable....... Very often, however, the actual purchase does not live up to the demands of doing good in the sustainable consumption chain, and the individual might end up with a guilty conscience, which again is a possible trigger for lingering in a sentimental mode of guilt. Emotions of sentimentality may actually...

  8. ATLAS IBL operational experience

    CERN Document Server

    Takubo, Yosuke; The ATLAS collaboration

    2016-01-01

    The Insertable B-Layer (IBL) is the inner most pixel layer in the ATLAS experiment, which was installed at 3.3 cm radius from the beam axis in 2014 to improve the tracking performance. To cope with the high radiation and hit occupancy due to proximity to the interaction point, a new read-out chip and two different silicon sensor technologies (planar and 3D) have been developed for the IBL. After the long shut-down period over 2013 and 2014, the ATLAS experiment started data-taking in May 2015 for Run-2 of the Large Hadron Collider (LHC). The IBL has been operated successfully since the beginning of Run-2 and shows excellent performance with the low dead module fraction, high data-taking efficiency and improved tracking capability. The experience and challenges in the operation of the IBL is described as well as its performance.

  9. Experimenting with practice

    DEFF Research Database (Denmark)

    Knudsen, Hanne; Adriansen, Hanne Kirstine

    2016-01-01

    these experiments the learning material and stepping stone for formulating problems in new ways. We argue that it is fruitful to make a distinction between practical problems and knowledge problems, and that playful shifts back and forth between the two forms of problems can provide learning. We also argue...... that it is important to observe the distinction between the role of the manager and the role of the student in order to meet ethical challenges, inevitably raised by experimenting with practice. Finally we argue that the experimental teaching practice can be conceptualised as a monstrous pedagogy, as the pedagogy...... creates a liminal zone with hybrid characteristics. Research limitations/implications The chapter provides new conceptualizations of the tensions between theory and practice based on our experiences from one degree programme. It would have been interesting to study other executive programmes and which...

  10. An Organoleptic Laboratory Experiment

    Science.gov (United States)

    Risley, John M.

    1996-12-01

    Flavorings in foods and fragrances in personal care products is a topic often discussed in chemistry classes designed for the general education of non-science majors. A laboratory experiment has been designed to accompany the lecture topic. Compounds in ten different classes of organic molecules that are used in the fragrance and food industry are provided to students. Students whiff the vapors of each compound and describe the organoleptic properties using a set of terms utilized in the fragrance and food industry. A set of questions guides students to an understanding of the relationship between structure of molecules and smell. Students are permitted to create their own fragrance based on the results of the experiment. Student response has been favorable. The experiment rectifies misconceptions students have about structure and odor, and gives positive reinforcement to the lecture material.

  11. Experiments in Particle Astrophysics

    Energy Technology Data Exchange (ETDEWEB)

    Boratav, M

    2004-07-01

    During the last decade, the field of what is currently called particle astrophysics (that I prefer to the shaky neologism astroparticle physics) has experienced a surprising growth. It is interesting to understand why the cosmic rays, the poor man's accelerator not no long ago, are becoming the object of scrutiny for a continuously growing community of theoreticians and experimentalists. In this article, we made an arbitrary choice of a small number of experiments to illustrate today's state of the art and the future perspectives in this domain. Our choice is based on three facts: the objects detected in each experiment are different, all the selected experiments are in their starting phase and all are spectacular for various reasons. Our aim is to convince the reader of the enormous discovery potential of these ongoing projects and share with him the excitement experienced by those involved in them. (Author) 37 refs.

  12. The Experiment as Act

    DEFF Research Database (Denmark)

    Søndergaard, Morten

    seminal experiments between poetry, sound and media. This paper will focus on the methodologies of ‘archiving’ experimental artforms. The ‘archive’, here, is argued to be in-between a gathering of objects and the simulation of time (Ernst). The archive presents one of the great cultural enigmas of our......In December 1965, an experiment took place at The Independent Art Space in Copenhagen (Den Frie Kunstbygning). Short named POEX65, it was looking to create and activate POetry EXperiments across artistic genres and formats (thus, in essence, making a POetry EXposition). The POEX65 event framed many...... time: It is constructed like a (sub)medium that collects our cultural history; at the same time, the archive often ‘forgets’ it’s own pre-experimental status and simply slips into a state of unconscious repetition which allows parallel histories to emerge in-between everything else. This paper tries...

  13. Cooperative Prototyping Experiments

    DEFF Research Database (Denmark)

    Bødker, Susanne; Grønbæk, Kaj

    1989-01-01

    This paper describes experiments with a design technique that we denote cooperative prototyping. The experiments consider design of a patient case record system for municipal dental clinics in which we used HyperCard, an off the shelf programming environment for the Macintosh. In the ecperiments we...... tried to achieve a fluent work-like evaluation of prototypes where users envisioned future work with a computer tool, at the same time as we made on-line modifications of prototypes in cooperation with the users when breakdown occur in their work-like evaluation. The experiments showed...... that it was possible to make a number of direct manipulation changes of prototypes in cooperation with the users, in interplay with their fluent work-like evaluation of these. However, breakdown occurred in the prototyping process when we reached the limits of the direct manipulation support for modification. From...

  14. Experiment SPHERE status 2008

    Energy Technology Data Exchange (ETDEWEB)

    Shaulov, S.B., E-mail: shaul@sci.lebedev.r [P.N.Lebedev Physical Institute, Russian Academy of Sciences, Leninsky prospect 53, Moscow 119991 (Russian Federation); Besshapov, S.P.; Kabanova, N.V.; Sysoeva, T.I. [P.N.Lebedev Physical Institute, Russian Academy of Sciences, Leninsky prospect 53, Moscow 119991 (Russian Federation); Antonov, R.A.; Anyuhina, A.M.; Bronvech, E.A.; Chernov, D.V.; Galkin, V.I. [Skobeltsyn Institute of Nuclear Physics, Lomonosov State University, Moscow 119992 (Russian Federation); Tkaczyk, W. [Department of Experimental Physics of University of Lodz (Poland); Finger, M. [Karlov University, Prague (Czech Republic); Sonsky, M. [COMPAS Consortium, Turnov (Czech Republic)

    2009-12-15

    The expedition carried out in March, 2008 to Lake Baikal became an important stage in the development of the SPHERE experiment. During the expedition the SPHERE-2 installation was hoisted, for the first time, on a tethered balloon, APA, to a height of 700 m over the lake surface covered with ice and snow. A series of test measurements were made. Preliminary results of the data processing are presented. The next plan of the SPHERE experiment is to begin a set of statistics for constructing the CR spectrum in the energy range 10{sup 16}-10{sup 18} eV.

  15. World Ocean Circulation Experiment

    Science.gov (United States)

    Clarke, R. Allyn

    1992-01-01

    The oceans are an equal partner with the atmosphere in the global climate system. The World Ocean Circulation Experiment is presently being implemented to improve ocean models that are useful for climate prediction both by encouraging more model development but more importantly by providing quality data sets that can be used to force or to validate such models. WOCE is the first oceanographic experiment that plans to generate and to use multiparameter global ocean data sets. In order for WOCE to succeed, oceanographers must establish and learn to use more effective methods of assembling, quality controlling, manipulating and distributing oceanographic data.

  16. Initial Cooling Experiment (ICE)

    CERN Multimedia

    Photographic Service

    1978-01-01

    In 1977, in a record-time of 9 months, the magnets of the g-2 experiment were modified and used to build a proton/antiproton storage ring: the "Initial Cooling Experiment" (ICE). It served for the verification of the cooling methods to be used for the "Antiproton Project". Stochastic cooling was proven the same year, electron cooling followed later. Also, with ICE the experimental lower limit for the antiproton lifetime was raised by 9 orders of magnitude: from 2 microseconds to 32 hours. For its previous life as g-2 storage ring, see 7405430. More on ICE: 7711282, 7809081, 7908242.

  17. The OPERA experiment

    CERN Document Server

    Pessard, H

    2001-01-01

    Following the Super-Kamiokande findings on atmospheric neutrinos, accelerator neutrinos have started to be exploited to confirm the indication for neutrino oscillations and perform more complete measurements of the mass differences and mixing parameters. In Europe, the long baseline beam CERN Neutrino to Gran Sasso CNGS is aimed at direct appearance searches of nu /sub mu / to nu /sub tau /. The OPERA experiment in Gran sasso will use photographic emulsions in a large hybrid detector to point at nu , interactions coming from the CNGS beam in a "zero background" approach. The principles of the experiment and its projected performances are described. (12 refs).

  18. Experiments versus simultations

    DEFF Research Database (Denmark)

    Baltser, Jana

    The world of instrumentation utilising X-ray radiation is expanding and leading to more sophisticated experiments. Every step of this process requires accurate calculation, and this is where simulation plays an important role. With the advert of modern computers and technologies, simulation has...... become a very powerful tool, which allows prediction of experimental outcomes with high precision and accuracy. The present Ph. D. work is dedicated to development of such a simulation tool based on a ray-tracing technique, McXtrace, its application for modelling of experiments at a synchrotron beamline...

  19. The Dialogue of Experience

    DEFF Research Database (Denmark)

    Jørgensen, Dorthe

    2016-01-01

    from ‘school philosophy’ or ‘applied philosophy.’ The concept of ‘world-engaged philosophy’ is associated with Jørgensen’s concept of ‘world poetry’: that the immanent world is ambiguous; it occasions experiences of a surplus of meaning, traditionally called beauty. Furthermore, both concepts......, theology, and philosophy, are thus interrelated. ‘Experience’ and ‘dialogue’ also play crucial roles in Vattimo’s understanding of philosophy, and recent works such as Hermeneutic Communism confirm that his thought continues to be world-engaged. Jørgensen’s philosophy of experience allows for a development...

  20. DEEP UNDERGROUND NEUTRINO EXPERIMENT

    Energy Technology Data Exchange (ETDEWEB)

    Wilson, Robert J. [Fermilab

    2016-03-03

    The Deep Underground Neutrino Experiment (DUNE) collaboration will perform an experiment centered on accelerator-based long-baseline neutrino studies along with nucleon decay and topics in neutrino astrophysics. It will consist of a modular 40-kt (fiducial) mass liquid argon TPC detector located deep underground at the Sanford Underground Research Facility in South Dakota and a high-resolution near detector at Fermilab in Illinois. This conguration provides a 1300-km baseline in a megawatt-scale neutrino beam provided by the Fermilab- hosted international Long-Baseline Neutrino Facility.

  1. The Digital Archive Experience

    DEFF Research Database (Denmark)

    2009-01-01

    This article, as well as the book, investigates the ways in which new digital media may enhance the experience of the art-archive. Taken as a whole, the new media is a vital component of a 'transdisciplinary' and transformative field, a cultural landscape that is changing rapidly the conditions...... on paper but seldom succeeds in reality. Such websites often ignore the physical and social experience of a museum visit. Curiously, when they are most successful, websites often compete with the actual museum, possibly reducing the number of visitors and diluting the effect of seeing art first hand...

  2. The Archimedes experiment

    CERN Document Server

    Calloni, Enrico; De Laurentis, Martina; Esposito, Giampiero; Grilli, M; Majorana, Ettore; Pepe, G P; Petrarca, S; Puppo, Paola; Rapagnini, P; Ricci, F; Rosa, Luigi; Rovelli, Carlo; Ruggi, P; Saini, N L; Stornaiolo, Cosimo; Tafuri, Francesco

    2015-01-01

    Archimedes is an INFN-funded pathfinder experiment aimed at verifying the feasibility of measuring the interaction of vacuum fluctuations with gravity. The final experiment will measure the force exerted by the gravitational field on a Casimir cavity whose vacuum energy is modulated with a superconductive transition, by using a balance as a small force detector. Archimedes is a two-year project devoted to test the most critical experimental aspects, in particular the balance resonance frequency and quality factor, the thermal modulation efficiency and the superconductive sample realization.

  3. The Archimedes experiment

    Science.gov (United States)

    Calloni, E.; Caprara, S.; Laurentis, M. De; Esposito, G.; Grilli, M.; Majorana, E.; Pepe, G. P.; Petrarca, S.; Puppo, P.; Rapagnani, P.; Ricci, F.; Rosa, L.; Rovelli, C.; Ruggi, P.; Saini, N. L.; Stornaiolo, C.; Tafuri, F.

    2016-07-01

    Archimedes is an INFN-funded pathfinder experiment aimed at verifying the feasibility of measuring the interaction of vacuum fluctuations with gravity. The final experiment will measure the force exerted by the gravitational field on a Casimir cavity whose vacuum energy is modulated with a superconductive transition, by using a balance as a small force detector. Archimedes is two-year project devoted to test the most critical experimental aspects, in particular the balance resonance frequency and quality factor, the thermal modulation efficiency and the superconductive sample realization.

  4. NA44 experiment

    CERN Document Server

    1990-01-01

    General view of NA44 experiment. NA44 is one of the seven experiments (NA44, NA45, NA49, NA50, NA52, WA97/NA57 and WA98) involved in CERN'S Heavy Ion programme which provided evidence for the existence of a new state of matter in which quarks, instead of being bound up into more complex particles such as protons and neutrons, are liberated and roam freely. Theory predicts that this state must have existed at about 10 microseconds after the Big Bang, before the formation of matter as we know it today.

  5. The Experience City

    DEFF Research Database (Denmark)

    Marling, Gitte; Jensen, Ole B.; Kiib, Hans

    2012-01-01

    findings. The projects are categorised according to their content, structure and urban localisation. In particular the cases are labelled in relation to their strategic and urban planning importance, their social and cultural content and their architectural representation and the programmes they contain...... to relate to the wider international debate and development. In section two we present the main theoretical concepts and framings that will guide the understanding and the analysis of the experience city. In section three we focus on the design of the ‘Danish experience city’ and present the first research...

  6. Belief Elicitation in Experiments

    DEFF Research Database (Denmark)

    Blanco, Mariana; Engelmann, Dirk; Koch, Alexander

    Belief elicitation in economics experiments usually relies on paying subjects according to the accuracy of stated beliefs in addition to payments for other decisions. Such incentives, however, allow risk-averse subjects to hedge with their stated beliefs against adverse outcomes of other decisions...... in the experiment. This raises two questions: (i) can we trust the existing belief elicitation results, (ii) can we avoid potential hedging confounds? Our results instill confidence regarding both issues. We propose an experimental design that eliminates hedging opportunities, and use this to test for the empirical...

  7. Future of neutrino experiments

    Indian Academy of Sciences (India)

    Takaaki Kajita

    2009-01-01

    Atmospheric, solar, reactor and accelerator neutrino oscillation experiments have measured $ m_{12}^{2}$, sin2 12, $| m_{23}^{2} |$ and sin2 223. The next stage of the oscillation studies should be the observation of a finite sin2 213. If a non-zero sin2 213 is observed, the subsequent goals should be the observation of the CP violation and the determination sign of $ m_{23}^{2}$. Possible future neutrino oscillation experiments that could assess these questions are discussed.

  8. The Archimedes experiment

    Energy Technology Data Exchange (ETDEWEB)

    Calloni, E. [University of Napoli Federico II and INFN Napoli (Italy); Caprara, S. [University of Roma Sapienza and INFN Roma (Italy); Laurentis, M. De; Esposito, G. [University of Napoli Federico II and INFN Napoli (Italy); Grilli, M.; Majorana, E. [University of Roma Sapienza and INFN Roma (Italy); Pepe, G.P. [University of Napoli Federico II and INFN Napoli (Italy); Petrarca, S. [University of Roma Sapienza and INFN Roma (Italy); Puppo, P., E-mail: paola.puppo@roma1.infn.it [University of Roma Sapienza and INFN Roma (Italy); Rapagnani, P.; Ricci, F. [University of Roma Sapienza and INFN Roma (Italy); Rosa, L. [University of Napoli Federico II and INFN Napoli (Italy); Rovelli, C. [University of Aix-Marseille (France); Ruggi, P. [European Gravitational Observatory (EGO), Cascina (Pisa) (Italy); Saini, N.L. [University of Roma Sapienza and INFN Roma (Italy); Stornaiolo, C.; Tafuri, F. [University of Napoli Federico II and INFN Napoli (Italy)

    2016-07-11

    Archimedes is an INFN-funded pathfinder experiment aimed at verifying the feasibility of measuring the interaction of vacuum fluctuations with gravity. The final experiment will measure the force exerted by the gravitational field on a Casimir cavity whose vacuum energy is modulated with a superconductive transition, by using a balance as a small force detector. Archimedes is two-year project devoted to test the most critical experimental aspects, in particular the balance resonance frequency and quality factor, the thermal modulation efficiency and the superconductive sample realization. - Highlights: • Weight of the vacuum. • Superconductive stacks studies. • Thermal behavior studies at cryogenic temperatures.

  9. AGS experiments, 1988, 1989, 1990

    Energy Technology Data Exchange (ETDEWEB)

    Depken, J.C.

    1991-04-01

    This report contains: experimental areas layout; table of beam parameters and fluxes; experiment schedule as run''; experiment long range schedule; a listing of experiments by number; two-page summaries of each experiment begin here, also ordered by number; publications of AGS experiments; and list of experimenters.

  10. AGS experiments: 1985, 1986, 1987

    Energy Technology Data Exchange (ETDEWEB)

    Depken, J.C.

    1987-01-01

    This report contains: Experimental areas layout, table of beam parameters and fluxes, experiment schedule ''as run,'' experiment long range schedule, a listing of experiments by number, two-page summaries of each experiment, also ordered by number, and publications of AGS experiments, 1982-1987.

  11. Art and experience

    NARCIS (Netherlands)

    Gerwen, R.C.H.M. van

    1996-01-01

    This three-part dissertation is on the double role of experience in art: as a subject matter, and as the vehicle for our evaluations. It argues (Part three, Chs. 7, 8) for the inclusion within contemporary analytical ‘cognitivism’ (Part one, Chs. 1-3) of certain arguments from the founding fathers

  12. Electronics for LHC Experiments

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2004-07-01

    This document gathers the abstracts of most presentations made at this workshop on electronics for the large hadron collider (LHC) experiments. The presentations were arranged into 6 sessions: 1) electronics for tracker, 2) trigger electronics, 3) detector control systems, 4) data acquisition, 5) electronics for calorimeters and electronics for muons, and 6) links, power systems, grounding and shielding, testing and quality assurance.

  13. The PADME experiment

    CERN Document Server

    ,

    2016-01-01

    The PADME experiment, hosted at the Laboratori Nazionali di Frascati, will search for a Dark Photon that decays in invisible channels with a mass up to $23.7\\,\\mbox{MeV}$ and coupling constant down to $10^{-3}$.

  14. Democratic design experiments

    DEFF Research Database (Denmark)

    Ehn, Pelle; Brandt, Eva; Halse, Joachim

    2016-01-01

    we here see design engagements which are both controversial in their commitment to agendas of social change and experimental in the sense that they openly probe for what can possibly be enacted. In this conversation we want to explore how such engagements may be seen as democratic design experiments...

  15. The big experiment

    CERN Multimedia

    MacEacheran, Mike

    2010-01-01

    "From an academic laboratory in Switzerland, Cern, the European Organisation for Nuclear Research, is conducting physics experiments that are mesmerising everyone. But are its scientitsts really going to answer the questions of life, the Univers and everyhing in between?" (4 pages)

  16. The COMPASS experiment

    CERN Multimedia

    Laurent Guiraud

    1999-01-01

    Detail of one of the magnets on the Common Muon and Proton Apparatus for Structure and Spectroscopy (COMPASS) experiment. COMPASS studies the structure of composite particles which interact via the strong force, called hadrons. These have a complicated internal structure due to the nature of their force carriers, gluons, which can form self interacting bunches called glueballs.

  17. Science and Human Experience

    Science.gov (United States)

    Cooper, Leon N.

    2015-01-01

    Part I. Science and Society: 1. Science and human experience; 2. Does science undermine our values?; 3. Can science serve mankind?; 4. Modern science and contemporary discomfort: metaphor and reality; 5. Faith and science; 6. Art and science; 7. Fraud in science; 8. Why study science? The keys to the cathedral; 9. Is evolution a theory? A modest proposal; 10. The silence of the second; 11. Introduction to Copenhagen; 12. The unpaid debt; Part II. Thought and Consciousness: 13. Source and limits of human intellect; 14. Neural networks; 15. Thought and mental experience: the Turing test; 16. Mind as machine: will we rubbish human experience?; 17. Memory and memories: a physicist's approach to the brain; 18. On the problem of consciousness; Part III. On the Nature and Limits of Science: 19. What is a good theory?; 20. Shall we deconstruct science?; 21. Visible and invisible in physical theory; 22. Experience and order; 23. The language of physics; 24. The structure of space; 25. Superconductivity and other insoluble problems; 26. From gravity to light and consciousness: does science have limits?

  18. The Alpha Antihydrogen Experiment

    Science.gov (United States)

    Madsen, N.; Andresen, G.; Bertsche, W.; Boston, A.; Bowe, P. D.; Butler, E.; Cesar, C. L.; Chapman, S.; Charlton, M.; Chartier, M.; Fajans, J.; Funakoshi, R.; Gill, D. R.; Hangst, J. S.; Hardy, W. N.; Hayano, R. S.; Hayden, M.; Hydomako, R.; Jenkins, M. J.; Jørgensen, L. V.; Kurchaninov, L.; Nolan, P.; Olchanski, K.; Olin, A.; Page, R. D.; Povilus, A.; Robicheaux, F.; Sarid, E.; Seif El Nasr, S.; Silveira, D. M.; Storey, J. W.; Thompson, R. I.; van der Werf, D. P.; Wurtele, J. S.; Yamazaki, Y.

    2008-03-01

    ALPHA is a new experiment at the CERN Antiproton Decelerator (AD). The short term goal of ALPHA is trapping of cold antihydrogen, with the long term goal of conducting precise spectroscopic comparisons of hydrogen and antihydrogen. Here we present the current status of ALPHA and the physics considerations and results leading to its design as well as recent progress towards trapping.

  19. Alpha Antihydrogen Experiment

    Science.gov (United States)

    Fujiwara, M. C.; Andresen, G. B.; Ashkezari, M. D.; Baquero-Ruiz, M.; Bertsche, W.; Bray, C. C.; Butler, E.; Cesar, C. L.; Chapman, S.; Charlton, M.; Cesar, C. L.; Fajans, J.; Friesen, T.; Gill, D. R.; Hangst, J. S.; Hardy, W. N.; Hayano, R. S.; Hayden, M. E.; Humphries, A. J.; Hydomako, R.; Jonsell, S.; Kurchaninov, L.; Lambo, R.; Madsen, N.; Menary, S.; Nolan, P.; Olchanski, K.; Olin, A.; Povilus, A.; Pusa, P.; Robicheaux, F.; Sarid, E.; Silveira, D. M.; So, C.; Storey, J. W.; Thompson, R. I.; van der Werf, D. P.; Wilding, D.; Wurtele, J. S.; Yamazaki, Y.

    2011-12-01

    ALPHA is an experiment at CERN, whose ultimate goal is to perform a precise test of CPT symmetry with trapped antihydrogen atoms. After reviewing the motivations, we discuss our recent progress toward the initial goal of stable trapping of antihydrogen, with some emphasis on particle detection techniques.

  20. Qualitative experiments in psychology

    DEFF Research Database (Denmark)

    Wagoner, Brady

    2015-01-01

    was in a state of transition from a focus on elements (the concern of psychophysics) to a focus on wholes (the concern of Gestalt psychology). The defining feature of BARTLETT's early experiments is his holistic treatment of human responses, in which the basic unit of analysis is the active person relating...

  1. Conducting Social Experiments.

    Science.gov (United States)

    Boruch, Robert F.

    1987-01-01

    It is difficult to conduct randomized field experiments. In the past decade, the use of alternative randomization plans and incentives has contributed to their operational feasibility; legal, ethical, and professional arguments for experimentation have matured; and expectations have become better aligned with practical constraints that are likely…

  2. Democratic design experiments

    DEFF Research Database (Denmark)

    Ehn, Pelle; Brandt, Eva; Halse, Joachim

    2016-01-01

    we here see design engagements which are both controversial in their commitment to agendas of social change and experimental in the sense that they openly probe for what can possibly be enacted. In this conversation we want to explore how such engagements may be seen as democratic design experiments...

  3. The Huck Finn Experience.

    Science.gov (United States)

    Levesque, Bonnie; And Others

    This paper describes "The Huck Finn Experience," a high motivation interdisciplinary unit that takes students on a 6-week journey back to the life and times of Mark Twain through the fictional character of Huckleberry Finn. The unit described in the paper was designed for eighth-grade students but could be easily adapted to be successful with…

  4. A Harmonic Motion Experiment

    Science.gov (United States)

    Gluck, P.; Krakower, Zeev

    2010-01-01

    We present a unit comprising theory, simulation and experiment for a body oscillating on a vertical spring, in which the simultaneous use of a force probe and an ultrasonic range finder enables one to explore quantitatively and understand many aspects of simple and damped harmonic motions. (Contains 14 figures.)

  5. Social experience infrastructure

    DEFF Research Database (Denmark)

    Kvistgaard, Peter

    2006-01-01

    Using the case of Kühlungsborn in Mecklenburg-Vorpommern as an example of a resort in which social experience infrastructure plays a pivotal role in the ongoing success of attracting German tourists from especially Berlin, Hamburg and Hanover it is the aim of this article in a descriptive...

  6. An Unhappy Experience.

    Science.gov (United States)

    Ali, Aydin Mehmet

    1998-01-01

    A study of 10 Cypriotturkish young people in British universities identified the factors they perceive as having had an impact on their achievement. Negative experiences in secondary and higher education included teachers' low expectations, racism, and lack of role models/mentors. Women were more aware of these issues than men were. (JOW)

  7. "Experience and Learning"

    DEFF Research Database (Denmark)

    Olesen, Henning Salling

    2007-01-01

    concepts derived from European critical theory, subjectivity and experience, are briefly introduced with a view to their intellectual background. The chapter elaborates the implication of these concepts in relation to the understanding of emotional aspects of learning in everyday work life and in relation...

  8. Belief Elicitation in Experiments

    DEFF Research Database (Denmark)

    Blanco, Mariana; Engelmann, Dirk; Koch, Alexander

    Belief elicitation in economics experiments usually relies on paying subjects according to the accuracy of stated beliefs in addition to payments for other decisions. Such incentives, however, allow risk-averse subjects to hedge with their stated beliefs against adverse outcomes of other decisions......-belief elicitation treatment using a financial investment frame, where hedging arguably would be most natural....

  9. A Monopoly Classroom Experiment.

    Science.gov (United States)

    Oxoby, Robert J.

    2001-01-01

    Uses a simple classroom experiment to develop the economic model of monopoly. Introduces students to the nature of the monopoly problem and motivates them to think of the associated effects. Highlights the role of information and fairness ideals in determining economic outcomes. (RLH)

  10. Outdoor Experiences and Sustainability

    Science.gov (United States)

    Prince, Heather E.

    2017-01-01

    Positive outdoor teaching and learning experiences and sound pedagogical approaches undoubtedly have contributed towards an understanding of environmental sustainability but it is not always clear how, and to what extent, education can translate into action. This article argues, with reference to social learning theory, that role modelling,…

  11. Enhance Your Twitter Experience

    Science.gov (United States)

    Miller, Shannon McClintock

    2010-01-01

    The author has been encouraging teachers, students, and others to join Twitter and build their personal learning networks (PLNs) ever since she delved into this great social networking site. In this article, she offers a few other tools and tips that can improve the Twitter experience of those who have opened up an account and dabbled a bit but…

  12. Condensed landscape experience

    DEFF Research Database (Denmark)

    Earon, Ofri

    2011-01-01

    . This paper addresses the question of whether the sensation of landscape can be condensed in function or to the size of an urban building. It also discusses the benefits and potentials of the amalgamate, by underlining the unique qualities of such a hybrid. In an attempt to define the experience of landscape...

  13. Experience of exclusion

    DEFF Research Database (Denmark)

    Pedersen, Maria; Overgaard, Dorthe; Andersen, Ingelise

    2017-01-01

    this phenomenon. The aim of the study was to explore the patient experience of barriers to completion of phase II cardiac rehabilitation, and to investigate the impact of socioeconomic factors on completion of cardiac rehabilitation. METHODS: The study had a qualitative explorative design using semi...

  14. The Transgender Military Experience

    Directory of Open Access Journals (Sweden)

    Michelle Dietert

    2015-04-01

    Full Text Available Although there have been studies that focus on the experiences of the gay and lesbian population serving in the United States military, few have focused on the experience of active duty transgender service members. Transgender individuals transgress the binary conception of gender by deviating from societal gender norms associated with assigned sex at birth. The Department of Defense has set policies and standards that reflect a binary conception of gender, with a focus on conformity. We argue that able-bodied gender variant service personnel are just as capable of serving their country as anyone else. Because of the repercussions associated with active duty transgender military personnel, our sample is small and involves nine clandestine service members and two international service members who wanted to share their stories from a different perspective. Snowball sampling was aimed at finding current active duty and reserve transgender service members. Using a combination of telephone interviews and questionnaires, data were collected from active duty transgender service personnel throughout the United States and two from international militaries that allow transgender people to serve. Data collection focused on the overall experiences of the participants along with questions regarding workplace discrimination, suggestions for policy changes, and their views about the overturn of Don’t Ask, Don’t Tell. Our findings add to a growing source of information about the transgender military experience in the U.S. armed forces and the importance of overturning discriminatory workplace policies that negatively impact transgender service members.

  15. Experiments on Photoconductivity

    Science.gov (United States)

    Kraftmakher, Yaakov

    2012-01-01

    Computer-assisted experiments with CdS and CdSe photoresistors are described. The most important characteristics of the photoresistors are determined: (i) the spectral response, (ii) the photocurrent versus incident radiant power, (iii) the rise and decay time constants and (iv) the frequency response to modulated light. The photoconductivity gain…

  16. Alpha Antihydrogen Experiment

    CERN Document Server

    Fujiwara, M C; Ashkezari, M D; Baquero-Ruiz, M; Bertsche, W; Bray, C C; Butler, E; Cesar, C L; Chapman, S; Charlton, M; Cesar, C L; Fajans, J; Friesen, T; Gill, D R; Hangst, J S; Hardy, W N; Hayano, R S; Hayden, M E; Humphries, A J; Hydomako, R; Jonsell, S; Kurchaninov, L; Lambo, R; Madsen, N; Menary, S; Nolan, P; Olchanski, K; Olin, A; Povilus, A; Pusa, P; Robicheaux, F; Sarid, E; Silveira, D M; So, C; Storey, J W; Thompson, R I; van der Werf, D P; Wilding, D; Wurtele, J S; Yamazaki, Y

    2011-01-01

    ALPHA is an experiment at CERN, whose ultimate goal is to perform a precise test of CPT symmetry with trapped antihydrogen atoms. After reviewing the motivations, we discuss our recent progress toward the initial goal of stable trapping of antihydrogen, with some emphasis on particle detection techniques.

  17. Understanding Popper's experiment

    Science.gov (United States)

    Qureshi, Tabish

    2005-06-01

    An experiment proposed by Karl Popper is considered by many to be a crucial test of quantum mechanics. Although many loopholes in the original proposal have been pointed out, they are not crucial to the test. We use only the standard interpretation of quantum mechanics to point out what is fundamentally wrong with the proposal, and demonstrate that Popper's basic premise was faulty.

  18. Neutrino Experiments Highlights

    CERN Document Server

    Wong, H T

    2001-01-01

    This article consists of two parts. The first section presents the highlights on the goals of neutrino physics, status of the current neutrino experiments and future directions and program. The second section describes the theme, program and research efforts for the TEXONO Collaboration among scientists from Taiwan and China.

  19. Experiments with Dipole Antennas

    Science.gov (United States)

    Kraftmakher, Yaakov

    2009-01-01

    Employment of a data-acquisition system for data collection and calculations makes experiments with antennas more convenient and less time consuming. The determined directional patterns of the dipole antennas of different lengths are in reasonable agreement with theory. The enhancement of the signal by using a reflector is demonstrated, and a…

  20. The Santabot Experiment

    DEFF Research Database (Denmark)

    2009-01-01

    The focus is to study people's willingness to interact, and their reaction when a robot enters their daily environment. The robot autonomously detects and follows people, while keeping a safe distance. The conclusion was that people were positive towards robots in their daily life. The experiments...

  1. Experiment R701

    CERN Multimedia

    1974-01-01

    This experiment was designed by the CERN-Aachen-MPI/Munich-Heidelberg Collaboration to study inelastic proton-proton collisions with streamer chambers. The photo shows the lower streamer chamber closely fitting around the central bicone vacuum chamber at I-7. The upper chamber, here removed, was similarly fitted (Photo Archive 7401099).

  2. Experiences of Collaborative Research

    Science.gov (United States)

    Kahneman, Daniel

    2003-01-01

    The author's personal history of the research that led to his recognition in economics is described, focusing on the process of collaboration and on the experience of controversy. The author's collaboration with Amos Tversky dealt with 3 major topics: judgment under uncertainty, decision making, and framing effects. A subsequent collaboration,…

  3. Caring Experience and Knowledge

    DEFF Research Database (Denmark)

    Dybbroe, Betina

    2005-01-01

    ,didactics and educational thinking- and students experiences and sensing, and illuminates excluding processes in classrooms related to emotional, non-cognitive and relational aspects of the qualifying process. This is set into a larger framework of the biographical professionalization processes of students...

  4. Cyclic Voltammetry Experiment.

    Science.gov (United States)

    Van Benschoten, James J.; And Others

    1983-01-01

    Describes a three-part experiment designed to introduce cyclic voltammetry to graduate/undergraduate students. Part 1 demonstrates formal reduction potential, redox electron transfer, diffusion coefficient, and electrochemical reversibility. Part 2 investigates electrochemical behavior of acetaminophen. Part 3 examines such experimental variables…

  5. Art and experience

    NARCIS (Netherlands)

    Gerwen, R.C.H.M. van

    2001-01-01

    This three-part dissertation is on the double role of experience in art: as a subject matter, and as the vehicle for our evaluations. It argues (Part three, Chs. 7, 8) for the inclusion within contemporary analytical ‘cognitivism’ (Part one, Chs. 1-3) of certain arguments from the founding fathers o

  6. A Simple Adsorption Experiment

    Science.gov (United States)

    Guirado, Gonzalo; Ayllon, Jose A.

    2011-01-01

    The study of adsorption phenomenon is one of the most relevant and traditional physical chemistry experiments performed by chemistry undergraduate students in laboratory courses. In this article, we describe an easy, inexpensive, and straightforward way to experimentally determine adsorption isotherms using pieces of filter paper as the adsorbent…

  7. FLORIDA TOWER FOOTPRINT EXPERIMENTS

    Energy Technology Data Exchange (ETDEWEB)

    WATSON,T.B.; DIETZ, R.N.; WILKE, R.; HENDREY, G.; LEWIN, K.; NAGY, J.; LECLERC, M.

    2007-01-01

    The Florida Footprint experiments were a series of field programs in which perfluorocarbon tracers were released in different configurations centered on a flux tower to generate a data set that can be used to test transport and dispersion models. These models are used to determine the sources of the CO{sub 2} that cause the fluxes measured at eddy covariance towers. Experiments were conducted in a managed slash pine forest, 10 km northeast of Gainesville, Florida, in 2002, 2004, and 2006 and in atmospheric conditions that ranged from well mixed, to very stable, including the transition period between convective conditions at midday to stable conditions after sun set. There were a total of 15 experiments. The characteristics of the PFTs, details of sampling and analysis methods, quality control measures, and analytical statistics including confidence limits are presented. Details of the field programs including tracer release rates, tracer source configurations, and configuration of the samplers are discussed. The result of this experiment is a high quality, well documented tracer and meteorological data set that can be used to improve and validate canopy dispersion models.

  8. The OLYMPUS experiment

    Energy Technology Data Exchange (ETDEWEB)

    Milner, R.; Hasell, D.K. [Massachusetts Institute of Technology, Cambridge, MA (United States); Kohl, M. [Hampton Univ., Hampton, VA (United States); Collaboration: The OLYMPUS Collaboration; and others

    2013-12-15

    The OLYMPUS experiment was designed to measure the ratio between the positron-proton and electron-proton elastic scattering cross sections, with the goal of determining the contribution of two-photon exchange to the elastic cross section. Two-photon exchange might resolve the discrepancy between measurements of the proton form factor ratio, {mu}{sub p}G{sup p}{sub E}/G{sup p}{sub M}, made using polarization techniques and those made in unpolarized experiments. OLYMPUS operated on the DORIS storage ring at DESY, alternating between 2.01 GeV electron and positron beams incident on an internal hydrogen gas target. The experiment used a toroidal magnetic spectrometer instrumented with drift chambers and time-of-flight detectors to measure rates for elastic scattering over the polar angular range of approximately 25 -75 . Symmetric Moeller/Bhabha calorimeters at 1.29 and telescopes of GEM and MWPC detectors at 12 served as luminosity monitors. A total luminosity of approximately 4.5 fb{sup -1} was collected over two running periods in 2012. This paper provides details on the accelerator, target, detectors, and operation of the experiment.

  9. Child Psychology Experiences Program.

    Science.gov (United States)

    Walla Walla Coll., WA.

    Recognizing the need for trained teachers to enter the classroom with confidence and professional capacity, Walla Walla College introduced a Child Psychology Experience program. Personnel from several departments contribute to this program. In connection with the child psychology courses, the project features a laboratory/demonstration center…

  10. The Doppler Pendulum Experiment

    Science.gov (United States)

    Lee, C. K.; Wong, H. K.

    2011-01-01

    An experiment to verify the Doppler effect of sound waves is described. An ultrasonic source is mounted at the end of a simple pendulum. As the pendulum swings, the rapid change of frequency can be recorded by a stationary receiver using a simple frequency-to-voltage converter. The experimental results are in close agreement with the Doppler…

  11. The Majorana Experiment

    Energy Technology Data Exchange (ETDEWEB)

    Aalseth, Craig E.; Aguayo Navarrete, Estanislao; Amman, M.; Avignone, F. T.; Back, Henning O.; Bai, Xinhua; Barabash, Alexander S.; Barbeau, P. S.; Bergevin, M.; Bertrand, F.; Boswell, M.; Brudanin, V.; Bugg, William; Burritt, Tom H.; Busch, Matthew; Capps, Greg L.; Chan, Yuen-Dat; Collar, J. I.; Cooper, R. J.; Creswick, R.; Detwiler, Jason A.; Diaz, J.; Doe, Peter J.; Efremenko, Yuri; Egorov, Viatcheslav; Ejiri, H.; Elliott, S. R.; Ely, James H.; Esterline, James H.; Farach, H. A.; Fast, James E.; Fields, N.; Finnerty, P.; Fraenkle, Florian; Gehman, Victor M.; Giovanetti, G. K.; Green, M.; Guiseppe, Vincente; Gusey, K.; Hallin, A. L.; Harper, Gregory; Hazama, R.; Henning, Reyco; Hime, Andrew; Hong, H.; Hoppe, Eric W.; Hossbach, Todd W.; Howard, Stanley; Howe, M. A.; Johnson, R. A.; Keeter, K.; Keillor, Martin E.; Keller, C.; Kephart, Jeremy D.; Kidd, M. F.; Knecht, A.; Kochetov, Oleg; Konovalov, S.; Kouzes, Richard T.; LaRoque, B. H.; Leviner, L.; Loach, J. C.; Luke, P.; MacMullin, S.; Marino, Michael G.; Martin, R. D.; Medlin, D.; Mei, Dong-Ming; Miley, Harry S.; Miller, M. L.; Mizouni, Leila; Myers, Allan W.; Nomachi, Masaharu; Orrell, John L.; Peterson, David; Phillips, D.; Poon, Alan; Perevozchikov, O.; Perumpilly, Gopakumar; Prior, Gersende; Radford, D. C.; Reid, Douglas J.; Rielage, Keith; Robertson, R. G. H.; Rodriguez, Larry; Ronquest, M. C.; Salazar, Harold; Schubert, Alexis G.; Shima, T.; Shirchenko, M.; Sobolev, V.; Steele, David; Strain, J.; Swift, Gary; Thomas, K.; Timkin, V.; Tornow, W.; Van Wechel, T. D.; Vanyushin, I.; Varner, R. L.; Vetter, Kai; Vorren, Kris R.; Wilkerson, J. F.; Wolfe, B. A.; Xiang, W.; Yakushev, E.; Yaver, Harold; Young, A.; Yu, Chang-Hong; Yumatov, V.; Zhang, C.

    2011-08-01

    The Majorana Collaboration is assembling an array of HPGe detectors to search for neutrinoless double-beta decay in 76Ge. Initially, Majorana aims to construct a prototype module to demonstrate the potential of a future 1-tonne experiment. The design and potential reach of this prototype Demonstrator module are presented.

  12. Electrophoresis experiments in microgravity

    Science.gov (United States)

    Snyder, Robert S.; Rhodes, Percy H.

    1991-01-01

    The use of the microgravity environment to separate and purify biological cells and proteins has been a major activity since the beginning of the NASA Microgravity Science and Applications program. Purified populations of cells are needed for research, transplantation and analysis of specific cell constituents. Protein purification is a necessary step in research areas such as genetic engineering where the new protein has to be separated from the variety of other proteins synthesized from the microorganism. Sufficient data are available from the results of past electrophoresis experiments in space to show that these experiments were designed with incomplete knowledge of the fluid dynamics of the process including electrohydrodynamics. However, electrophoresis is still an important separation tool in the laboratory and thermal convection does limit its performance. Thus, there is a justification for electrophoresis but the emphasis of future space experiments must be directed toward basic research with model experiments to understand the microgravity environment and fluid analysis to test the basic principles of the process.

  13. The NA48 experiment

    CERN Multimedia

    1995-01-01

    The NA48 experiment is designed to study the Charge-Parity (CP) violation. CP violation is observed in certain very rare decays. Its study is important in understanding Nature's preference of matter over antimatter, which resulted in asymmetry soon after the Big Bang, creating a matter-dominated Universe.

  14. Modelling Urban Experiences

    DEFF Research Database (Denmark)

    Jantzen, Christian; Vetner, Mikael

    2008-01-01

    How can urban designers develop an emotionally satisfying environment not only for today's users but also for coming generations? Which devices can they use to elicit interesting and relevant urban experiences? This paper attempts to answer these questions by analyzing the design of Zuidas, a new...

  15. Parent Hearing Aid Experiences

    Science.gov (United States)

    Munoz, Karen; Roberts, Mallory; Mullings, Day; Harward, Richard

    2012-01-01

    This study addresses parent experiences in obtaining and managing hearing aids for their young child. The purpose was to identify challenges parents encounter to determine what state agencies can do to improve parent access to amplification. Data were collected July through September of 2010; 40 parents of children ages birth to 3 years old…

  16. Experiments versus simultations

    DEFF Research Database (Denmark)

    Baltser, Jana

    become a very powerful tool, which allows prediction of experimental outcomes with high precision and accuracy. The present Ph. D. work is dedicated to development of such a simulation tool based on a ray-tracing technique, McXtrace, its application for modelling of experiments at a synchrotron beamline...

  17. Cyclic Voltammetry Experiment.

    Science.gov (United States)

    Van Benschoten, James J.; And Others

    1983-01-01

    Describes a three-part experiment designed to introduce cyclic voltammetry to graduate/undergraduate students. Part 1 demonstrates formal reduction potential, redox electron transfer, diffusion coefficient, and electrochemical reversibility. Part 2 investigates electrochemical behavior of acetaminophen. Part 3 examines such experimental variables…

  18. The MAJORANA Experiment

    Energy Technology Data Exchange (ETDEWEB)

    Guiseppe, V.E. [Univ S Dakota; Keller, C. [Univ S Dakota; Mei, D-M [Univ S Dakota; Perevozchikov, O. [Univ S Dakota; Perumpilly, G. [Univ S Dakota; Thomas, K. [Univ S Dakota; Xiang, W. [Univ S Dakota; Zhang, C. [Univ S Dakota; Aalseth, C.E. [Pacific NW Natl Lab Richland, WA; Aguayo, E. [Pacific NW Natl Lab Richland, WA; Ely, J. [Pacific NW Natl Lab Richland, WA; Fast, J.E. [Pacific NW Natl Lab Richland, WA; Hoppe, E.W. [Pacific NW Natl Lab Richland, WA; Hossbach, T.W. [Pacific NW Natl Lab Richland, WA; Keillor, M. [Pacific NW Natl Lab Richland, WA; Kephart, J.D. [Pacific NW Natl Lab Richland, WA; Kouzes, R. [Pacific NW Natl Lab Richland, WA; Miley, H.S. [Pacific NW Natl Lab Richland, WA; Mizouni, L. [Pacific NW Natl Lab Richland, WA; Myers, A.W. [Pacific NW Natl Lab Richland, WA; Reid, D. [Pacific NW Natl Lab Richland, WA; Amman, M. [Lawrence Berkeley National Laboratory (LBNL); Bergevin, M. [Lawrence Berkeley National Laboratory (LBNL); Chan, Y-D [Lawrence Berkeley National Laboratory (LBNL); Detwiler, J.A. [Lawrence Berkeley National Laboratory (LBNL); Loach, J.C. [Lawrence Berkeley National Laboratory (LBNL); Luke, P.N. [Lawrence Berkeley National Laboratory (LBNL); Martin, R.D. [Lawrence Berkeley National Laboratory (LBNL); Poon, A.W.P. [Lawrence Berkeley National Laboratory (LBNL); Prior, G. [Lawrence Berkeley National Laboratory (LBNL); Vetter, K. [Lawrence Berkeley National Laboratory (LBNL); Yaver, H. [Lawrence Berkeley National Laboratory (LBNL); Avignone, F.T. III [University of South Carolina; Creswick, R. [University of South Carolina; Farach, H. [University of South Carolina; Mizouni, L. [University of South Carolina; Avignone, Frank Titus [ORNL; Bertrand Jr, Fred E [ORNL; Capps, Gregory L [ORNL; Cooper, Reynold J [ORNL; Radford, David C [ORNL; Varner Jr, Robert L [ORNL; Wilkerson, John F [University of North Carolina, Chapel Hill; Yu, Chang-Hong [ORNL; Back, H.O. [University of North Carolina; Leviner, L. [North Carolina State University; Young, A.R. [North Carolina State University; Back (et al.), H.O. [Triangle Universities Nuclear Laboratory, Durham, NC; Bai, X. [South Dakota School of Mines and Technology; Hong, H. [South Dakota School of Mines and Technology; Howard, S. [South Dakota School of Mines and Technology; Medlin, D. [South Dakota School of Mines and Technology; Sobolev, V. [South Dakota School of Mines and Technology; Barabash, A.S. [Inst Theort & Expt Phys, Moscow, Russia; Konovalov, S.I. [Inst Theort & Expt Phys, Moscow, Russia; Vanyushin, I. [Inst Theort & Expt Phys, Moscow, Russia; Yumatov, V. [Inst Theort & Expt Phys, Moscow, Russia; Barbeau, P.S. [University of Chicago; Collar, J.I. [University of Chicago; Fields, N. [University of Chicago; Boswell (et al.), M. [Los Alamos National Laboratory (LANL); Brudanin, V. [Joint Institute for Nuclear Research, Dubna, Russia; Egorov, V. [Joint Institute for Nuclear Research, Dubna, Russia; Gusey, K. [Joint Institute for Nuclear Research, Dubna, Russia; Kochetov, O. [Joint Institute for Nuclear Research, Dubna, Russia; Shirchenko, M. [Joint Institute for Nuclear Research, Dubna, Russia; Timkin, V. [Joint Institute for Nuclear Research, Dubna, Russia; Yakushev, E. [Joint Institute for Nuclear Research, Dubna, Russia; Bugg, W. [University of Tennessee, Knoxville (UTK); Efremenko, M. [University of Tennessee, Knoxville (UTK); Burritt (et al.), T.H. [University of Washington, Ctr Expt Nucle Phys & Astrophys; Burritt (et al.), T.H. [University of Washington, Dept Phys, Seattle, WA; Busch, M. [Duke University; Esterline, J. [Duke University; Swift, G. [Duke University; Tornow, W. [Duke University/TUNL; Ejiri, H. [Osaka University; Hazama, R. [Osaka University; Nomachi, M. [Osaka University; Shima, T. [Osaka University; Finnerty (et al.), P. [University of North Carolina; et al.

    2011-01-01

    The Majorana Collaboration is assembling an array of HPGe detectors to search for neutrinoless double-beta decay in {sup 76}Ge. Initially, Majorana aims to construct a prototype module to demonstrate the potential of a future 1-tonne experiment. The design and potential reach of this prototype Demonstrator module are presented.

  19. Parabolic aircraft solidification experiments

    Science.gov (United States)

    Workman, Gary L. (Principal Investigator); Smith, Guy A.; OBrien, Susan

    1996-01-01

    A number of solidification experiments have been utilized throughout the Materials Processing in Space Program to provide an experimental environment which minimizes variables in solidification experiments. Two techniques of interest are directional solidification and isothermal casting. Because of the wide-spread use of these experimental techniques in space-based research, several MSAD experiments have been manifested for space flight. In addition to the microstructural analysis for interpretation of the experimental results from previous work with parabolic flights, it has become apparent that a better understanding of the phenomena occurring during solidification can be better understood if direct visualization of the solidification interface were possible. Our university has performed in several experimental studies such as this in recent years. The most recent was in visualizing the effect of convective flow phenomena on the KC-135 and prior to that were several successive contracts to perform directional solidification and isothermal casting experiments on the KC-135. Included in this work was the modification and utilization of the Convective Flow Analyzer (CFA), the Aircraft Isothermal Casting Furnace (ICF), and the Three-Zone Directional Solidification Furnace. These studies have contributed heavily to the mission of the Microgravity Science and Applications' Materials Science Program.

  20. Remote Agent Experiment

    Science.gov (United States)

    Benard, Doug; Dorais, Gregory A.; Gamble, Ed; Kanefsky, Bob; Kurien, James; Millar, William; Muscettola, Nicola; Nayak, Pandu; Rouquette, Nicolas; Rajan, Kanna; Norvig, Peter (Technical Monitor)

    2000-01-01

    Remote Agent (RA) is a model-based, reusable artificial intelligence (At) software system that enables goal-based spacecraft commanding and robust fault recovery. RA was flight validated during an experiment on board of DS1 between May 17th and May 21th, 1999.

  1. The ATRAP experiment

    CERN Multimedia

    Laurent Guiraud

    2000-01-01

    The Antihydrogen Trap (ATRAP) experiment was the first machine to accumulate cold antiprotons, produced in the AD, and combine them with positrons (antielectrons) to form antihydrogen. The study of antihydrogen in comparison with hydrogen will hopefully provide insight into the differences between matter and antimatter, especially in comparing their mass and spectra.

  2. Invisalign: early experiences.

    Science.gov (United States)

    Joffe, L

    2003-12-01

    This article describes the Invisalign technique. It is based on the author's personal experience of over 60 cases started in the private practice setting. The technology behind Invisalign and its development is reviewed. The Invisalign clinical technique is described, and the advantages and disadvantages of using Invisalign are highlighted.

  3. Dismantling the ALEPH experiment

    CERN Multimedia

    Patrice Loïez

    2001-01-01

    The barrel and its two end-caps have been separated and removed from their cables; the detector will be broken into individual parts. ALEPH was an experiment on the LEP accelerator, which studied high-energy collisions between electrons and positrons from 1989 to 2000.

  4. Experiment at Evergreen.

    Science.gov (United States)

    Jones, Richard M.

    The philosophy and structure of programs of coordinated study at Evergreen State College from 1970 to 1980 are discussed, and comparisons are made to the experiment at the University of California, Berkeley, promoted by Joseph Tussman. Both programs are based on ideas suggested by Alexander Meiklejohn and his Experimental College at the University…

  5. Transformations of emotional experience.

    Science.gov (United States)

    de Cortiñas, Lia Pistiner

    2013-06-01

    In this paper the author approaches mental pain and the problems in a psychoanalytic treatment of patients with difficulties in the psychic transformation of their emotional experiences. The author is interested in the symbolic failure related to the obstruction of development of phantasies, dreams, dream-thoughts, etc. She differentiates symbolization disturbances related to hypertrophic projective identification from a detention of these primitive communications and emotional isolation. She puts forward the conjecture that one factor in the arrest of this development is the detention of projective identifications and that, when this primitive means of communication is re-established in a container-contained relationship of mutual benefit, this initiates the development of a symbolization process that can replace the pathological 'protection'. Another hypothesis she develops is that of inaccessible caesuras that, associated with the detention of projective identification, obstruct any integrative or interactive movement. This caesura and the detention of projective identifications affect mental functions needed for dealing with mental pain. The personality is left with precarious mental equipment for transforming emotional experiences. How can a psychoanalytical process stimulate the development of creative symbolization, transforming the emotional experiences and leading towards mental growth? The author approaches the clinical problem with the metaphor of the psychic birth of emotional experience. The modulation of mental pain in a container-contained relationship is a central problem for the development of the human mind. For discovering and giving a meaning to emotional experience, the infant depends on reverie, a function necessary in order to develop an evolved consciousness capable of being aware, which is different from the rudimentary consciousness that perceives but does not understand. The development of mature mental equipment is associated with the

  6. Hydrothermal organic synthesis experiments

    Science.gov (United States)

    Shock, Everett L.

    1992-01-01

    The serious scientific debate about spontaneous generation which raged for centuries reached a climax in the nineteenth century with the work of Spallanzani, Schwann, Tyndall, and Pasteur. These investigators demonstrated that spontaneous generation from dead organic matter does not occur. Although no aspects of these experiments addressed the issue of whether organic compounds could be synthesized abiotically, the impact of the experiments was great enough to cause many investigators to assume that life and its organic compounds were somehow fundamentally different than inorganic compounds. Meanwhile, other nineteenth-century investigators were showing that organic compounds could indeed be synthesized from inorganic compounds. In 1828 Friedrich Wohler synthesized urea in an attempt to form ammonium cyanate by heating a solution containing ammonia and cyanic acid. This experiment is generally recognized to be the first to bridge the artificial gap between organic and inorganic chemistry, but it also showed the usefulness of heat in organic synthesis. Not only does an increase in temperature enhance the rate of urea synthesis, but Walker and Hambly showed that equilibrium between urea and ammonium cyanate was attainable and reversible at 100 C. Wohler's synthesis of urea, and subsequent syntheses of organic compounds from inorganic compounds over the next several decades dealt serious blows to the 'vital force' concept which held that: (1) organic compounds owe their formation to the action of a special force in living organisms; and (2) forces which determine the behavior of inorganic compounds play no part in living systems. Nevertheless, such progress was overshadowed by Pasteur's refutation of spontaneous generation which nearly extinguished experimental investigations into the origins of life for several decades. Vitalism was dealt a deadly blow in the 1950's with Miller's famous spark-discharge experiments which were undertaken in the framework of the Oparin

  7. LCLS The First Experiments

    Energy Technology Data Exchange (ETDEWEB)

    Galayda, John N

    2003-02-10

    The Scientific Advisory Committee (SAC) for the Linac Coherent Light Source (LCLS) has selected six scientific experiments for the early phase of the project. The LCLS, with proposed construction in the 2003-2006 time frame, has been designed to utilize the last third of the existing Stanford Linear Accelerator Center (SLAC) linac. The linac produces a high-current 5-15 GeV electron beam that is bunched into 230 fs slices with a 120 Hz repetition rate. When traveling through a sufficiently long (of order of 100 m) undulator, the electron bunches will lead to self amplification of the emitted x-ray intensity constituting an x-ray free electron laser (XFEL). If funded as proposed, the LCLS will be the first XFEL in the world, operating in the 800-8,000 eV energy range. The emitted coherent x-rays will have unprecedented brightness with 10{sup 12}-10{sup 13} photons/pulse in a 0.2-0.4% energy bandpass and an unprecedented time structure with a design pulse length of 230 fs. Studies are under way to reduce the pulse length to tens of femtoseconds. This document presents descriptions of the early scientific experiments selected by SAC in the spring of 2000. They cover a wide range of scientific fields. The experimental teams consist of many internationally recognized scientists who are excited about the unprecedented x-ray capabilities of LCLS that surely will lead to new scientific frontiers. More generally, this document serves to forward the scientific case for an accelerator-based XFEL source, as requested by the BESAC subpanel on Novel Coherent Light Sources, chaired by Stephen R. Leone. Two general classes of experiments are proposed for the LCLS. The first class consists of experiments where the x-ray beam is used to probe the sample without modifying it, as is done in most experiments at current synchrotron sources. In the second class, the LCLS beam is used to induce non-linear photo-processes or matter in extreme conditions. The same source can be used for

  8. Experiments around I-8

    CERN Multimedia

    1975-01-01

    The lithium transition-radiation detectors and the large liquid argon calorimeters of experiment R806T are shown above and below the intersection at I-8 (Brookhaven-CERN-Saclay-Syracuse-Yale Collaboration, Study of large transverse momentum phenomena by electron and photon detection). At 90 deg to the intersecting beams are the monitoring proporional chambers of experiment R805 (Measurement of real to imaginary ratio of forward scattering amplitude - Coulomb interference - by the CERN-Rome Collaboration). Left and right of the intersection one sees, symmetrically placed around the interaction region, the large scintillation counters hodoscopes used by R801 (Pisa-Stony Brook Collaboration) to measure the pp total cross section and the features of inelastic collisions.

  9. Infrastructures as Ontological Experiments

    Directory of Open Access Journals (Sweden)

    Casper Bruun Jensen

    2015-11-01

    Full Text Available Ontology has recently gained renewed attention in science and technology studies and anthropology (e.g. Gad, Jensen and Winthereik 2015; Holbraad, Pedersen and Viveiros de Castro 2014; Woolgar and Lezaun 2013. Yet, it has a considerably longer pedigree than these recent debates might lead one to think. Experiments, of course, have long held the attention of sociologists, historians, and philosophers of science (Collins 1985; Gooding 1990; Shapin and Schaffer 1985. And infrastructures have been the focus of sustained inquiry in the sociology and history of technology (Bowker 1994; Hughes 1983. Once these terms are put into conjunction, however, each gets a somewhat different inflection. The following note briefly explores the conceptual purchase of considering infrastructures as ontological experiments.

  10. Quantum Gravity Experiments

    Directory of Open Access Journals (Sweden)

    Cahill R. T.

    2015-10-01

    Full Text Available A new quantum gravity experiment is reported with the data confirming the generali- sation of the Schrödinger equation to include the interaction of the wave function with dynamical space. Dynamical space turbulence, via this interaction process, raises and lowers the energy of the electron wave function, which is detected by observing conse- quent variations in the electron quantum barrier tunnelling rate in reverse-biased Zener diodes. This process has previously been reported and enabled the measurement of the speed of the dynamical space flow, which is consistent with numerous other detection experiments. The interaction process is dependent on the angle between the dynamical space flow velocity and the direction of the electron flow in the diode, and this depen- dence is experimentally demonstrated. This interaction process explains gravity as an emergent quantum process, so unifying quantum phenomena and gravity. Gravitational waves are easily detected.

  11. The ATLAS experiment

    CERN Document Server

    Dunford, Monica Lynn

    2014-01-01

    In particle physics experiments, the discovery of increasingly more massive particles has brought deep understanding of the basic constituents of matter and of the fundamental forces among them. In order to explore Nature in its deepest elementary secrets, the Large Hadron Collider (LHC) was built at CERN, Geneva. The LHC provides the highest energy collisions in a laboratory, at very high rates to allow one to study very rare reactions. Two independent sophisticated huge instruments, called ATLAS and CMS detectors, are operated to explore in a most broad way the physics of these collisions. In addition to these two general-purpose detectors, smaller specialized experiments (LHCb, ALICE and some others) are collecting collision data as well.

  12. The Digital Archive Experience

    DEFF Research Database (Denmark)

    2009-01-01

    This article, as well as the book, investigates the ways in which new digital media may enhance the experience of the art-archive. Taken as a whole, the new media is a vital component of a 'transdisciplinary' and transformative field, a cultural landscape that is changing rapidly the conditions...... on paper but seldom succeeds in reality. Such websites often ignore the physical and social experience of a museum visit. Curiously, when they are most successful, websites often compete with the actual museum, possibly reducing the number of visitors and diluting the effect of seeing art first hand....... The book discusses the challenges of the archive and the (art)museum in the age of digital media. It is based upon documentation from a research project, MAP - Media Art Platform, that drew upon the talents and collaboration of many institutions, artists, programmers, art historians, designers and others...

  13. Shooting Star Experiment

    Science.gov (United States)

    1997-01-01

    The Shooting Star Experiment (SSE) is designed to develop and demonstrate the technology required to focus the sun's energy and use the energy for inexpensive space Propulsion Research. Pictured is an engineering model (Pathfinder III) of the Shooting Star Experiment (SSE). This model was used to test and characterize the motion and deformation of the structure caused by thermal effects. In this photograph, alignment targets are being placed on the engineering model so that a theodolite (alignment telescope) could be used to accurately measure the deformation and deflections of the engineering model under extreme conditions, such as the coldness of deep space and the hotness of the sun as well as vacuum. This thermal vacuum test was performed at the X-Ray Calibration Facility because of the size of the test article and the capabilities of the facility to simulate in-orbit conditions

  14. Future flavour physics experiments

    Science.gov (United States)

    2015-01-01

    The current status of flavour physics and the prospects for present and future experiments will be reviewed. Measurements in B‐physics, in which sensitive probes of new physics are the CKM angle γ, the Bs mixing phase ϕs, and the branching ratios of the rare decays B(s)0→μ+μ− , will be highlighted. Topics in charm and kaon physics, in which the measurements of ACP and the branching ratios of the rare decays K→πνν¯ are key measurements, will be discussed. Finally the complementarity of the future heavy flavour experiments, the LHCb upgrade and Belle‐II, will be summarised. PMID:26877543

  15. Gross decontamination experiment report

    Energy Technology Data Exchange (ETDEWEB)

    Mason, R.; Kinney, K.; Dettorre, J.; Gilbert, V.

    1983-07-01

    A Gross Decontamination Experiment was conducted on various levels and surfaces of the TMI - Unit 2 reactor building in March 1982. The polar crane, D-rings, missile shields, refueling canals, refueling bridges, equipment, and elevations 305' and 347'-6'' were flushed with low pressure water. Additionally, floor surfaces on elevation 305' and floor surfaces and major pieces of equipment on elevation 347'-6'' were sprayed with high pressure water. Selective surfaces were decontaminated with a mechanical scrubber and chemicals. Strippable coating was tested and evaluated on equipment and floor surfaces. The effectiveness, efficiency, and safety of several decontamination techniques were established for the large, complex decontamination effort. Various decontamination equipment was evaluated and its effectiveness was documented. Decontamination training and procedures were documented and evaluated, as were the support system and organization for the experiment.

  16. The COMPASS experiment

    CERN Multimedia

    2006-01-01

    Artistic view of the 60 m long Common Muon and Proton Apparatus for Structure and Spectroscopy (COMPASS) experiment. COMPASS takes beams from the SPS accelerator at CERN. This beam enters from the lower left and passes through the cylindrical target solenoid. COMPASS is used to study the internal structure of hadrons (composite particles interacting via the strong force) by looking at a property called spin and a phenomenon known as glueballs.

  17. The ACE experiment

    CERN Multimedia

    Maximilien Brice

    2006-01-01

    The Antiproton Cell Experiment (ACE) as shown by Michael Holzscheiter (spokesperson), Niels Bassler (co-spokesperson) and Helge Knudsen. ACE is located on the Antiproton Decelerator (AD) at CERN. An antiproton annihilates a proton in the nucleus of a cancer cell, producing a pair of gamma rays, destroying the entire cell and some surrounding cells. Many fewer antiprotons are required in this treatment than in the equivalent proton hadron therapy, so there is less risk of healthy tissue damage.

  18. TEAM Experience in Astronomy

    Science.gov (United States)

    McGruder, C. H., III; Tyson, N.; Williams, B.; Hackney, K.; Hackney, R.; Rudloff, M.; Scott, R.; Tyler, R.

    1996-05-01

    The purpose of the project was to increase minority interest and appreciation of science using astronomy as a tool. This goal was achieved by inviting high school minority students and their teachers to the campus of Western Kentucky University to participate along with minority role models in team based learning experiences. All participants worked together in many hands-on activities and events centered around the exploration of space.

  19. The CONNIE experiment

    CERN Document Server

    Aguilar-Arevalo, A; Bonifazi, C; Butner, M; Cancelo, G; Vazquez, A Castaneda; Vergara, B Cervantes; Chavez, C R; Da Motta, H; D'Olivo, J C; Anjos, J Dos; Estrada, J; Moroni, G Fernandez; Ford, R; Foguel, A; Torres, K P Hernandez; Izraelevitch, F; Kavner, A; Kilminster, B; Kuk, K; Lima, H P; Makler, M; Molina, J; Moreno-Granados, G; Moro, J M; Paolini, E E; Haro, M Sofo; Tiffenberg, J; Trillaud, F; Wagner, S

    2016-01-01

    The CONNIE experiment uses fully depleted, high resistivity CCDs as particle detectors in an attempt to measure for the first time the Coherent Neutrino-Nucleus Elastic Scattering of antineutrinos from a nuclear reactor with silicon nuclei.This talk, given at the XV Mexican Workshop on Particles and Fields (MWPF), discussed the potential of CONNIE to perform this measurement, the installation progress at the Angra dos Reis nuclear power plant, as well as the plans for future upgrades.

  20. The CONNIE experiment

    Energy Technology Data Exchange (ETDEWEB)

    Aguilar-Arevalo, A.; et al.

    2016-10-19

    The CONNIE experiment uses fully depleted, high resistivity CCDs as particle detectors in an attempt to measure for the first time the Coherent Neutrino-Nucleus Elastic Scattering of antineutrinos from a nuclear reactor with silicon nuclei.This talk, given at the XV Mexican Workshop on Particles and Fields (MWPF), discussed the potential of CONNIE to perform this measurement, the installation progress at the Angra dos Reis nuclear power plant, as well as the plans for future upgrades.