The effects of electroacupuncture on leukocytes and plasma protein on the X ray-irradiated rats were investigated in the present study. The results showed that X-irradiation had an evident inhibitory effect on the counts of total leukocytes, lymphocytes and neutrocytes, and the concentration of the total plasma protein, plasma albumin, globulin and alpha- and beta-globulin in X-irradiated rats. The electroacupuncture was able to help the X-irradiated rats to recover the counts of the total leukocyte, lymphocyte and neutrocyte. The electroacupuncture had a helpful tendency to recover the concentration of the total plasma protein, albumin, globulin, and alpha- and beta-globulin in the irradiated rats
The effects of electroacupuncture on leukocytes and plasma protein on the X ray-irradiated rats were investigated in the present study. The results showed that X-irradiation had an evident inhibitory effect on the counts of total leukocytes, lymphocytes and neutrocytes, and the concentration of the total plasma protein, plasma albumin, globulin and alpha- and beta-globulin in X-irradiated rats. The electroacupuncture was able to help the X-irradiated rats to recover the counts of the total leukocyte, lymphocyte and neutrocyte. The electroacupuncture had a helpful tendency to recover the concentration of the total plasma protein, albumin, globulin, and alpha- and beta-globulin in the irradiated rats.
Polushina, N D; Kozhevnikov, S A; Makarov, V A; Vergeĭchik, E N; Kartazaeva, V A; Liubchik, V E
Before modelling of experimental ulcer according to I. S. Zavodskaia the animals (157 male Wistar rats) were given for 24 days mineral water Essentuki N 17 and rutin in a dose 20 or 40 mg. Those given 20 mg of rutin in combination with mineral water demonstrated a higher rise in blood concentrations of hydrocortisone, insulin and thyroxine. Gastric mucosa and levels of serum alpha-1, alpha-2, beta-globulins and albumins were less damaged.
Dokholyan, V K; Akhmedova, T P
Rutilus was subjected to various concentrations of mercury dichloride (100, 10, and 5 micrograms/ml). Brain tissue was then tested to determine nitrogen and carbon metabolism. All the fish in the highest concentration died, while those in the latter groups showed marked changes in metabolism. Ammonia content increased 135% in the first day, then dropped. Data on nitrogen metabolites are presented in two tables. For young fish (1 year), the ammonia content only increased 79%. Protein content in blood was reduced by 20 to 22% within 3 days then started to increase. There was an increase in alpha and beta globulins and a drop in blood sugar and liver glycogen.
EL-Sherbiny, E.M.; El-Mahdy, A.A.; Bayoumi, M.M.
The aim of this study was to clarify the effect of low and high dietary protein on some biochemical and hematological parameters in blood of female albino rats. A total number of 75 albino female rats were equally divided into 3 groups, the first group was fed 20% protein diet and served as control and the second and third groups were fed 5% and 65% protein for 5 weeks and served as low and high protein dietary groups, respectively. The results showed high significant decreases in serum growth hormone, ferritin levels and iron concentration in group II and there was significant increase in unsaturated iron binding capacity (UIBC) in group III, compared to control group. Studies of total protein and its fractions revealed high significant decreases in total protein, albumin, alpha-1-globulin, beta-globulin as well as gamma globulin in group II and significant increases in total protein, alpha-1- globulin, beta-globulin and gamma-globulin in group III, compared to normal control group. The hematological investigations in group II revealed significant decreases in hemoglobin value, total leukocyte count, platelets, mean corpuscular hemoglobin concentration (MCHC), erythrocytic count and mean corpuscular volume (MCV). On the other hand, there was significant increase in total leukocyte count in group III if compared to control group
Pye, Geoffrey W; Ellis, William; Fitzgibbon, Sean; Opitz, Brian; Keener, Laura; Arheart, Kristopher L; Cray, Carolyn
In a clinical setting, especially with species of special interest, it is important to use all clinical pathology testing options for general health monitoring and diagnosis. Protein electrophoresis (EPH) has previously been shown to be an important adjunct tool in veterinary medicine. Serum samples from 18 free-ranging and 12 zoo-based koalas (Phascolarctos cinereus) were subject to EPH analysis. Significant differences were found between the two groups for the following values: total protein, albumin, beta globulins, and albumin-globulin ratio (P < 0.05). By using the combined data, the minimum-maximum values for the EPH fractions were as follows: total protein 5.0-7.8 g/dl, albumin 2.8-4.7 g/dl, alpha-1 globulins 0.5-1.1 g/dl, alpha-2 globulins 0.3-0.7 g/dl, beta globulins 0.4-1.0 g/dl, gamma globulins 0.2-1.0 g/dl, and albumin-globulin ratio 1.0-2.1.
Roudiere, L; Boularan, A M; Bonardet, A; Vallat, C; Cristol, J P; Dupuy, A M
Capillary zone electrophoresis of serum proteins is increasingly gaining impact in clinical laboratories. During 2003, we compared the fully automated capillary electrophoresis (CE) system from Beckman (Paragon CZE 2000) with the method agarose gel electrophoresis Sebia (Hydrasis-Hyris, AGE). This new study focused on the evaluation of analytical performance and a comparison including 115 fresh routine samples (group A) and a series of 97 frozen pathologic sera with suspicion of monoclonal protein (group B). Coefficients of variation (CVs %) for the five classical protein fractions have been reported to be consistenly serum samples (group B), there were 90 in which we detected a monoclonal protein by immunofixation (IF) (immunosubtraction (IS) was not used). AGE and Paragon 2000 failed to detect 7 and 12 monoclonal proteins, respectively, leading to a concordance to 92% for AGE and 87% for Paragon 2000 for identifying electrophoretic abnormalities in this group. Beta-globulin abnormalities and M paraprotein were well detected with Paragon 2000. Only 81% (21 vs 26) of the gammopathies were immunotyped with IS by two readers blinded to the IF immunotype. The Paragon 2000 is a reliable alternative to conventional agarose gel electrophoresis combining the advantages of full automation (rapidity, ease of use and cost) with high analytical performance. Qualified interpretation of results requires an adaptation period which could further improve concordance between the methods. Recently, this CE system has been improved by the manufacturer (Beckman) concerning the migration buffer and detection of beta-globulin abnormalities.
Mansour, M.A.; Roushdy, H.M.; Mazhar, F.M.; Abu-Gabal, H.A.
A total number of 180 mature rats (120 females and 60 males) weighing from 120-140 g were used to study the effect of two doses (2 and 4 Gy) whole-body gamma irradiation on the level of total protein and protein fractions in serum of pregnant rats during the period of organogenesis. It was found that the levels of total protein, albumin and gamma globulins significantly decreased according to the doses of exposure. The levels of alpha and beta globulins significantly increased more in the serum of rats exposed to 2 Gy than in rats exposed to 4 Gy. The level of A/G ratio significantly decreased more in the serum of rats exposed to 2Gy than in those exposed to 4 Gy
Kocan, R.M.; Amend, S.R.
An epizootic of trichomoniasis in mourning doves at the Carolina Sandhills National Wildlife Refuge began in 1969 and continued into 1970. The disease was seen in 16% of the adults and 2% of the immatures in 1970, but only one immature bird out of 37 surveyed (3%) carried Trichomonas gallinae. Challenge infection of 33 doves from the epizootic area showed 85 percent to be resistant to trichomoniasis, compared to 69 percent resistance in doves from Maryland, where no epizootic has occurred for at least 3 years. Plasma protein changes which occurred following challenge infection were identical in CSNWR and Maryland doves which showed evidence of disease. Of the doves which showed no signs of disease, those from the CSNWR exhibited no change in beta globulins, identical to the response in pigeons which survive an infection by T. gallinae, but they had some tissue invasion by the parasite.
Do Hee Kim
Full Text Available Fanconi syndrome (FS is a rare condition that is characterized by defects in the proximal tubular function. A 48-year-old woman was admitted for evaluation of proteinuria. The patient showed normal anion gap acidosis, normoglycemic glycosuria, hypophosphatemia, and hypouricemia. Thus, her condition was compatible with FS. The M peak was found behind the beta globulin region in urine protein electrophoresis. Upon bone marrow examination, we found that 24% of cells were CD138+ plasma cells with kappa restriction. From a kidney biopsy, we found crystalline inclusions within proximal tubular epithelial cells. Thereafter, she was diagnosed with FS accompanied by multiple myeloma. The patient received chemotherapy and autologous stem cell transplantation, and obtained very good partial hematologic response. However, proximal tubular dysfunction was persistent until 1 year after autologous stem cell transplantation. In short, we report a case of FS accompanied by multiple myeloma, demonstrating crystalline inclusion in proximal tubular cells on kidney biopsy.
Full Text Available Acute plasma cell leukemia with Bence-Jones proteinuria is reported in a 60 year old lranien male with a 25 day history of acute onset of fever. weakness, weight loss, diarrhea and bloody stools. The patient was noted to be cachectic and anemic. He had purpuric and petechial skin lesions, generalized lymphadenopathy and splenomegaly. Up to 80% immature plasma cells were present in the peripheral blood and the platelet count was 10,000. Bone marrow was hypercellular and that most of it was composed of immature plasma cells. Serum electrophoresis showed increased beta globulins and Bence-Jones protein was strongly positive in the urine. The patient died after nine days in uremic coma with haemorrhagic diathesis. Auto psy showed wide spread infi ltra tion of plasmocytes and plasmocytoblasts in all organs.
Full Text Available Analyses of haematological and biochemical constituents were carried out on the Norwegian subspecies of free-ranging red deer (Cervus elaphus atlanticus. All animals were captured from January to March by using a mixture of xylazine and tiletamin-zolazepam. Immobilisation was performed with plastic projectile syringes fired from a dart gun. Fourteen haematological parameters were analysed. There were no differences in the values between hinds and stags and between adults and calves (P > 0.01. Of the 22 biochemical compounds investigated there was a significant difference (P < 0.01 between calves and adults for lactate dehydrogenase (LD, globulin, beta globulin, gamma globulin, and the minerals Na, K, Mg, Zn, Ca, and P. Differences (P < 0.01 between hinds and stags were found in cholesterol, gamma glutamyl transferase (GGT, alpha-1 globulin, alpha-2 globulin and Cu. The blood values determined in this study can be used as reference values for this red deer subspecies immobilised with a mixture of xylazine-tiletamin-zolazepam for health control and diagnosis of diseases.Abstract in Norwegian /Sammendrag:Hematologiske og biokjemiske parametere er analysert på norsk frittlevende hjort (Cervus elaphus atlanticus. Hjorten ble immobilisert i tidsrommet januar til mars ved hjelp av et spesialgevær ladet med plast kanyler som inneholdt en blanding av xylazin og tiletamin-zolazepam. Det var ingen forskjeller i de14 undersøkte hematologiske verdiene mellom hinder, kalver og bukker (P>0,01. Av de 22 biokjemiske parametrene som ble undersøkt var det en signifikant forskjell mellom kalver og voksne (P<0,01 når det gjelder laktat dehydrogenase, globulin, beta globulin, gamma globulin og mineralene Na, K, Mg, Zn, Ca og P. Det var en signifikant forskjell mellom hinder og bukker (P<0.01 på parametrene kolesterol, gamma glutamyl transferase, alfa-1 globulin, alfa-2 globulin og Cu. Blodverdiene som ble målt i dette studiet kan bli brukt som referanseverdier
Ichimaru, M; Yasuhi, S; Ouchuru, S
Since 1958, there have been 12 cases of multiple myeloma in Nagasaki, and among them were 7 cases representing atomic bombing casualties, with 3 cases being with 2 km distance from the hypocenter. The age of onset was between 51 and 69 years, and the sex ratio was 8:4, it occurring mostly in males. Symptoms were predominantly low back pain and chest pain caused by the bone changes in 8 cases. Two cases complained of general malaise and palpitation which resulted from anemia. One developed persistent epistaris, and another complained of diplopia caused by the paralysis of the oculomotor nerve. Peripheral blood in all cases showed anemia, 9 with hyperchromic and 3 with normochromic or hypochromic anemia. Low platelet counts were seen in 3 cases. All showed leukopenia. All cases showed typical ..gamma..-globulin change with a myeloma peak, and in 4 cases showed an increase of ..beta..-globulin. Bence-Jones proteinuria was present in 5 cases. Average course was 1 year 4 months. Among complications, myeloma nephrosis, aplastic anemia, and pneumonia were the most important ones.
Stram, D.O.; Akiba, S.; Neriishi, K.; Stevens, R.G.; Hosoda, Y.
Associations of smoking habit with serum levels of total protein as well as protein fractions were studied in a population consisting of 4,739 atomic-bomb survivors and unexposed control subjects in Hiroshima, Japan who participated in the 1979-1981 period of the Adult Health Study, an ongoing health follow-up program of the Radiation Effects Research Foundation. Smoking was strongly related to serum protein concentration after correction for age, sex, and body mass index. Among current smokers, levels of total protein, beta globulin, and gamma globulin were significantly lower and levels of alpha-1 and alpha-2 globulin were significantly higher, when compared with nonsmokers. For serum albumin levels a decrease was also noted, but it failed to attain statistical significance. Ex-smokers were indistinguishable from nonsmokers in terms of the serum protein levels analyzed. With an increase of the amount of daily cigarette consumption, monotonic increases of serum levels were observed only in alpha-1 globulin. Duration of smoking was related to increased alpha-1 and alpha-2 globulin. Smoking duration was also associated with albumin level, but the trend was not monotonic. The radiation exposure effect on serum protein level was significant in several instances but was in general much smaller than the smoking effect, and its inclusion in the regression models did not noticeably affect the association between smoking and serum proteins
Full Text Available ABSTRACT The aim of this study was to determine the effects of the replacement of different levels of protein derived from soybean meal with that from peas in broiler diets on serum protein fractions. A corn-soybean meal basal diet was formulated as the control diet (Control=C (NRC, 1994, and then pea was added to the control diet to replace 20% (P20 or 40% (P40 of the crude protein of the control diet. The diets were randomly fed to 12 pens per treatment, each housing five birds, for 42 days. Blood samples were collected from 36 birds (3 birds x 4 pens x3 treatments and the serum protein fractions were separated. Gamma-globulin percentage was higher in group P20 compared with C and P40 groups. Total protein, beta-globulin, and gamma-globulin concentrations were significantly higher in group P20 compared with those of both control and P40 group (p<0.05.
Wafaa Abdou Mohamed Mohamed
Full Text Available Objective: The aim of this study was to study the immunomodulatory activity improvement of levamisole by using lactoferrin when applied to immunosuppressed rat model. Methods: The study was designed as follows, 140 male albino rats (250-280 g 14 weeks old were used in our work. Rats were randomly divided into seven groups, 20 in each. The group I was kept as a control, group II was given cyclophosphamide (CYP at a single intraperitoneal dose of (250 mg/kg body weight, group III CYP and lactoferrin (Lac treated group, group IV orally administrated Lac only (0.5% in drinking water, group V treated with CYP and levamisole, group VI administrated levamisole orally at a dose of (2.5 mg/kg body weight and group VII was given CYP, Lac and levamisole. Animals were sacrificed and two separate blood samples were collected after 21 days from the beginning of the experiment for measuring the total and differential leukocyte count, serum total proteins, albumin, alpha globulin, beta globulin and gamma globulin, Nitric oxide (NO production and lysozyme activity. Results: CYP group showed significant decrease in the above mentioned parameters, which were improved after administration of both lactoferrin and levamisole. Conclusion: Our study concluded that lactoferrin improve the immunostimulant effect of levamisole in CYP- immunosuppressed rats. J Clin Exp Invest 2014; 5 (1: 48-53
Bibi, S.; Sajjad, I.; Akram, W.; Viqar, N.; Iqbal, M.S.; Iqbal, M.Z.
Serum of one hundred thirty normal subjects and 100 patients of different infections were analysed by electrophoresis and atomic absorption flame spectrophotometric technique. It was found that about sixty percent of zinc is bound to albumin and thirty percent is bound to alpha 2-globulins with a variation of 2-5%, beta-globulins and gamma-globulins with a variation of 2-5% in different normal subjects. While the determination of zinc in patients of different infections shows 20-30% decrease in the concentration of albumin bound zinc. The concentration of zinc in alpha 2-globulins remains the same in normal subjects as well as in patients of different infections. The results indicate that in low nourished normal subjects, there is a low concentration of zinc complexes of albumin and a greater chance of different infectious diseases it was further known that albumin works for the transport of zinc and possibly, alpha 2-globulins plays on intermediate role for the attachment of zinc to different body organs. From the above determinations, it is clear that it is the low concentration of zinc in normal subjects which favours the attack of infectious disease. So higher levels of zinc in serum means more resistance towards the attack of infectious diseases. (author)
SEVERIANA CÂNDIDA MEDONÇA CUNHA CARNEIRO
Full Text Available An adequate diet is important and nutritional errors may lead to irrevocable consequences. Nowadays, obesity is the most common nutritional disease in dogs, with a prevalence of 1 6%. In dogs, the level of physical activity, diet composition, food taste, and lifestyle are the most important factors which contribute to obesity. This study was carried out in order to assess the effects ofoverfeeding on clinical biochemistry and hematology of healthy puppies. Fourteen male Great Dane dogs at 10 weeks of age were studied during 27 weeks. The experimental design was completely randomized, with two treatments, seven replications, and six months of duration. Treatments were made up of super premium diet given ad libitum (GI (treatment 1 and restrictedly (GII (treatment 2. The evaluation of blood and urinary biochemistry as well as the determination ofhematological parameters were carried out. It was concluded that overfed puppies revealed seric elevations of alpha1 globulin, and lower values of beta globulin, gamma globulin, ALT, ALP, and creatinine. In blood evaluation, higher values of lymphocytes and lower values in the erythrogram were observed. The urinaryprotein:urinary creatinine value did not reveal any difference between treatments.
Anne Grace Silva Siqueira Campos
Full Text Available This work was carried out to evaluate the influence of administration of propilene glycol, cobalt and vitamin B12, on the electrophoretic profile of serum proteins on sheep and their offspring. The study was conducted using 18 pregnant ewes which were randomly divided into groups at 30 days before the date scheduled for delivery in order to be given supplements until the date preceding the birth. The groups were as follows: Group 1 (G1/n=6 Control Group; Group 2 (G2/n=6 Cobalt and Vitamin B12 (wich was received 1mg of cobalt orally daily and 2mg of vitamin B12, intramuscularly weeckly; and Group 3 (G3/n=6 Propylene Glycol (administration of 30mL of propylene glycol orally daily. We observed that mosto f the protein fractions vary with age development, particularly total serum protein, albumin, beta-globulin and gamma-globulin, and the determinant factor for these changes is colostrum intake. Furthermore, there was no influence of intake by sheep of the components studied on the profile of these variables in lambs.
ABDALLA, E.B.; EL-MASRY, K.A.; TEAMA, F.E.; EMARA, S.S.
This experiment was carried out under hot environmental conditions, where temperature-humidity index was equivalent to 86 - 90 and 78 - 80 during day and night, respectively. Twelve bovine Baladi calves of 8 - 10 months old and 112 kg average initial live body weight were used in this study. The calves were divided into two groups of 6 animals each to study the effect of supplementation of dried whey milk on some blood biochemical and immunological indices and growth performance of calves under hot weather conditions of Egypt. The results showed that supplementation of dried whey milk to the diet of heat-stressed calves at the level of 150 g / calf / day reduced significantly each of respiration rate and rectal temperature as well as serum lipid concentrations and their fractions e.g. total cholesterol and phospholipids. Also, dried whey milk supplement caused a significant decline in both AST and ALT activities and reduced significantly alpha globulin concentration, while non-significant changes were observed in each of beta globulin, gamma globulin and immunoglobulin G. However, supplementing dried whey milk to growing calves increased significantly serum concentrations of total protein, albumin, calcium, phosphorous, T 3 and T 4 . Moreover, dried whey milk improved significantly both feed efficiency and daily gain of growing calves. It could be concluded that addition of dried whey milk to the diet reduced rectal temperature and respiration rate and induced an improvement in most blood biochemical parameters and growth performance of heat-stressed bovine Baladi calves.
Ghys, R.; Reuter, A.
Delayed changes of the serum proteins in male Sprague Dawley rats that survived the acute radiation syndrome were investigated. Doses of Co/sup 60/ gamma ranging from LD/sub O/ to LD/sub 50/ were given to rats six to eight weeks of age. Paper electrophoreses and microdosage of proteins by the buiret method were performed on plasma proteins for 206 rats: 29 with acute irradiation; 73 chronic irradiation; 44 acute irradiation following cold acclimatization; and 80 normal animals. No significant variations in the total serum proteins were found in andy one group. Alpha globulins were found to be slightly above normal in some irradiated rats, but there was no significant variation in the BETA globulin fraction. Gamma globulins showed a marked and consistent increase following irradiation. Thus for observed protein chandges in irradiated rats have not proven to be dose dependent. It is suggested that the changes may provide a link between early irradiation syndrome and late effects. (H.M.G.)
Full Text Available We present the first description of amiodarone toxicity in the liver without phospholipidosis or steatosis. In doing so, we will review the various effects of amiodarone toxicity in various organs. The patient is a young adult who had cardiac reconstruction as a child for transposition of the great vessels. A needle biopsy was taken due to elevated liver enzymes. Her ALT was 188 U/L (5–50 and AST 162 U/L (5–50. Alkaline phosphatase, total bilirubin, protein, and albumin were within normal limits. A serologic panel for viral hepatitis was negative. Antinuclear antibodies were positive at 260; however, anti-smooth muscle antibody and anti-mitochondrial antibody were negative. A protein electrophoresis showed a slightly elevated beta globulin 2 level of 0.5. Quantitative immunoglobulin levels were within normal limits except for a slightly elevated IgA 409 mg/dL (60–350. Liver ultrasound was unremarkable. The clinical differential was broad and included hepatic congestion along with autoimmune hepatitis. Sections showed only ballooned hepatocytes with Mallory-Denk bodies and perisinusoidal fibrosis. Arrival to the diagnosis was possible only after careful review of the patient’s medications. After discontinuation of amiodarone, the patient’s liver enzymes returned to normal levels.
Study on the plasma proteins of A-bomb survived patients including those suffered by the remained radioactivities. Report 2. Quantitative observation of the plasma protein fractions by electrophoretic test and to solve the problems for physiological clinical significance of its patterns
Makidono, J; Takanashi, S; Yoshimoto, T; Kai, T; Yoshimoto, K; Matsutani, M; Miura, M
The plasma proteins of A-bombed survivors, healthy persons, long term x-ray equipment handling people (for instance the radiologists and x-ray technicians), cancer patients, and tumor irradiated cancer patients were examined by the electrophoretic test. It was found that the electrophoretic patterns of plasma proteins could be divided into normal (N-pattern) and abnormal (..beta.. and ..gamma.. patterns) patterns, when they were classified according to the accents of each fraction. The patterns of the healthy persons and the long term x-ray handling people showed normal (N) pattern, however, it showed 43% abnormal patterns in A-bombed survivors and 48% in cancer patients. Furthermore, the patterns could be changed by radiotherapy to cancer, ie., from N to ..beta.. or vice versa. As a result of the quantitative observation about individual pattern, the accents of ..beta..-globulins in ..beta..-patterns and ..gamma..-globulins in ..gamma..-patterns were found. The globulins increased in the A bomb survivors and the long term x-ray handling people, and this increase was also seen in the cases of cancer patients which showed 85% of them were effected with uclers (self disintegrated) by clinical examinations. A physiological clinical significance of these abnormal patterns (..beta.. and ..gamma..) in the plasma proteins indicates the disorders in its body and an important immunological meaning. Abnormal patterns in those who suffered by the remained radioactivities caused by the A-bomb showed 70%, whose average was much higher than those of direct A-bombed survivors. It is pointed out that, in recent days, there is a trend of more and gradual increase in the malignant neoplamsm than the disorders of direct A-bombed survivors.
Moore, A Russell; Allender, Matthew C; MacNeill, Amy L
Ranavirus is an emerging disease that infects fish, amphibians, and reptiles. Ranavirus induces an inflammatory response leading to death in many susceptible species. Red-eared sliders (RES; Trachemys scripta elegans) are vulnerable to ranavirus infection and are economically significant chelonians kept in the pet trade and utilized in research. Early identification of RES with inflammatory diseases would allow for isolation of affected individuals and subsequent disease investigation, including molecular testing for ranavirus. Validation of an inexpensive, clinically relevant, and reproducible diagnostic test that detects inflammation in turtles is needed. Although commonly used, plasma protein electrophoresis to detect an inflammatory acute-phase protein response has not been evaluated in a controlled environment in turtles with experimentally induced inflammatory disease. The objective of this study was to measure plasma protein fractions by electrophoresis to determine if an acute-phase protein response occurs in RES during infection with a frog virus 3-like ranavirus (FV3-like virus) isolated from a chelonian. A Bradford assay and agarose gel electrophoresis (AGE) were performed using plasma collected during a study of the effect of temperature on the pathogenesis of ranavirus in RES. In RES at the time of viremia, total albumin (ALB(mg/ml)) and albumin to globulin ratio were significantly lower and beta-globulin percentage was significantly higher in RES exposed to ranavirus (n = 4) as compared to matched, uninfected RES (n = 8). In the last sample collected prior to death, total protein (TP(mg/ml)), ALB(mg/ml), alpha-globulin percentage, and total alpha-globulin (alpha(mg/ml)) were significantly lower in RES exposed to ranavirus (n = 4) than control individuals (n = 8). In summary, FV3-like virus induces a decrease in plasma albumin concentration at the onset ofviremia and decreases in TP(mg/ml, ALB(mg/ml), and alpha(mg/ml) concentrations prior to death in
Glasgow, J.E.; Colman, R.W.
Fibronectin is a family of immunologically similar glycoproteins which mediate a variety of cell-cell and cell-substratum interactions. It is a constituent of the extracellular matrix of connective tissue and circulates in plasma. When suspension and adherent cultures of a human hepatoma cell line (SK-HEP-1) were incubated in serum-free medium, the resulting conditioned medium contained material which was specifically immunoprecipitated by antisera to human plasma fibronectin. By double immunodiffusion, a component in the conditioned culture medium was shown to form a line of identity with fibronectin in human plasma and to migrate as an alpha 2- to beta-globulin during immunoelectrophoresis. Human fibronectin was quantified in conditioned medium by electroimmunodiffusion, and was found to increase for at least three days at about 0.1 micrograms/10(6) cells/day. Adherent cultures of SK-HEP-1 cells were incubated with L-[ 35 S]methionine to label newly synthesized proteins. Labeled fibronectin in conditioned medium or in cell extracts comigrated with fibronectin in human plasma as shown by autoradiography following crossed-immunoelectrophoresis. Fibronectin was demonstrated in the extra-cellular matrix of adherent SK-HEP-1 cultures by immunofluorescence. It was shown previously that SK-HEP-1 cells synthesize alpha 1-protease inhibitor, one of the products of normal hepatocytes. The finding that these hepatoma cells also synthesize fibronectin supports the concept that the hepatocyte may be one source of circulating fibronectin, a possibility consistent with the established role of this cell type in blood plasma protein synthesis
Chi Cuiping; Wang Haiyan; Wei Jinping; Guo Jianping; Li Shufang; Tian Rong; Guo Fengling; Liu Huifang
In order to identify biomarkers for early diagnosis and/or for therapeutic targets in the delayed health effects of ionizing radiation, we analyzed the subgroups of lymphocytes, serum protein levels and gene expression profiles in the peripheral blood of three 60 Co γ-ray accidentally exposed persons during the three years after irradiation. Flow cytometry analyses and agarose gel electrophoresis were applied to investigate the subgroups of lymphocytes and the composition of serum proteins, respectively. Gene expression profiling was obtained using a whole genome gene expression chip assay. Both the percentage of CD4+ T lymphocytes and the ratio of Th to Ts were reduced compared with the normal control values. The percentage of albumin decreased whereas beta globulin increased. There were 285 up-regulated and 446 down-regulated genes in irradiated samples relative to the control samples. The expression of KDR, CEACAM8 and OSM was validated by RT-PCR. The majority of the differentially expressed genes encode proteins associated with the immune response, inflammation, oncogenesis, cell structure, oxidative stress, neuro-hormone regulation, reproduction, susceptibility to psychiatric disorders, or transcriptional regulation. We have identified a number of promising novel candidates that have potential for serving as biomarkers for delayed damage. Furthermore, the changes in the immunological indicator CD4+ T cells, and the ratio of CD4+ T to CD8+ T cells may be biomarkers for the prediction of delayed damage by ionizing radiation. The findings of our study are useful for forming a comprehensive understanding of the mechanisms underlying the delayed effects of ionizing radiation. (author)
Full Text Available Div-salaar K1, Saravani R2, Shamsi-e-meimandi M3, Taei M4, Sheikholeslami A5 1. MSc. Staff member of Neurology Sciences Research Center, Kerman University of Medical Sciences 2. Instructor, Department of Biochemistry, Faculty of Medicine, Zahedan University of Medical Sciences 3. Instructor, Department of Physiology and Pharmacology, Faculty of Medicine, Neurology Sciences Research Center, Karman University of Medical Sciences 4. Researcher, Neurology Sciences Research Center, Karman University of Medical Sciences 5. B.Sc in Environmental Hygiene, Kerman University of Medical Sciences Abstract Background: The prevalence rate of opioids consumption is high in Iran. The latest research approach related to substance abuse considers the role of plasma proteins in novel treatments of addiction. Since long-term consumption of opioids has some effects on liver function and plasma transfer systems, the present study was designed to determine the electrophoretic profile of plasma proteins in opiates-addict subjects. Materials and methods: In this cross-control study, the sample groups consisted of 42 opium consumers and 35 heroine dependents as case group and 35 non-addict volunteers as control group. The control group was matched with addicts for age and sex. Opioid consumption was confirmed by laboratory diagnostic tests on urine samples such as immunochromatography (RSA, rapidosis and complementary tests including liquid-solid column chromatography and thin layer chromatography (TLC. After blood collection and serum preparation, serum electrophoresis was performed. Data were presented as mean±SEM and analyzed by SPSS ver.11.5. The comparison of groups was done by using parametric tests and p<0.01 was considered as statistically significant. Results: There was no significant difference in the amounts of albumin, alpha-1-globulin, alpha-2-globulin and beta-globulin between groups. Gamma-globulin concentration was not significantly different between
Estudo eritroleucométrico e proteinograma sérico do sangue do cordão umbilical e jugular de eqüinos ao nascimento e de suas respectivas mães Erythrocytometry and seric proteinogram of umbilical cord and jugular of foals at birth and respective mothers
count (9.75x10(6/µ, hemoglobin determination (14.65g/dl, and mean corpuscular hemoglobin concentration (37.23g/dl; but lower values of mean corpuscular volume (40.50fl, total protein (4.37g/dl, alpha-globulin (0.65 g/dl, beta-globulin (1.10g/dl, gamma-globulin (0g/dl, total (5.40x10³/µl, and differential leukocytes count, except for band neutrophils and monocytes counts, when compared to the blood of the mares.
Friedrich, Mariola; Kuchlewska, Magdalena
Contemporarily, food production without food additives is very rare. Increasingly often, however, scientific works report on adverse effects of specified, single food additives on the body. Data is, in turn, lacking on the synergistic effect of a mixture of different food additives on body functions and its main metabolic pathways. The objective of this study, an animal model, was to evaluate if and in what way the compound of chosen and most frequently used and consumed food additives, along with the change of diet composition to processed, purified, influence the selected markers of protein metabolism. The animals were divided into four groups, which were fed with compound of feed pellets: group I and II with basic compound, group III and IV with modified compound in which part of the full grain was replaced by isocalorie wheat flour type 500 and saccharose. Animals from groups I and III received tap water, which was standing for some time, to drink. Animals from groups II and IV received solution of chosen additives to food and next they were given water to drink. The amount of given food additives was evaluated by taking into consideration their consumption by people recalculated to 1 kg of their body mass. The experiment spanned for 7 weeks. It was ascertained that the applied additives caused significant changes in total protein concentration and its fractions: albumin, alpha1-globulin, alpha2-globulin, beta-globulin and gamma-globulin in the blood serum of the animals under research, which can indicate and contribute to disclosure of creation of undesirable food reaction, especially when recommended levels of consumption of those additives are being exceeded. The organism response to the applied additives and accompanying it change of diet was essentially connected to sex of the animals. Undesirable character of changes taking place under the influence of applied additives, was observed both in animals fed with basic feed and modified feed with various
. So, the evaluation of the changes that may be considered proper to the CSF in the post-mortem was carried out only in the samples presenting no more then 1,000 hematiae per mml. Normal white cells number was proportionally more common in the third group of cases. Pleocytosis was more frequently found than normal cell count. It was slight or discrete in most of the cases. More than 50 white cells per mml were found in the samples collected from cases in which the patients had died in the course of acute infections. Low concentrations of chloride and glycosis were found in most of the cases and they tended to be lower with the increase of TOC. Hipoglycorrhachia was found to be more intense in the cases with pleocytosis. The concentration of urea was high in most of the cases; the increase of urea contents was at the same range in the three groups of cases considered. High GOT and GPT activities were found in several cases and the TOC appeared to influence the increase of these activities. This influence was more conspicuous in the last group only for GPT. Total protein concentrations above normal were frequent. They tended to be more conspicuously elevated with the increasing of TOC. The protein profiles were marked by the inversion of albumin/globulins ratio. They tended to be of the mixed type, with increase in the alpha and/or gamma globulins participation. The gamma globulin increase was more frequently observed. The increase of these globulin fractions was more conspicuous in the cases refering to patients who died during infectious diseases. An increase of the beta globulin fraction was observed in four cases: in two the death occurred in the course of hepatic cyrrhosis and, in two, of neoplasies. The influence of TOC on the protein profile changes was discrete: with the increase of the TOC the participation of gamma globulin increased and that of beta globulin decreased. The aspects of CSF composition here considered allow the conclusion that many of the changes
Efeito do miriadenolídeo isolado de Alomia myriadenia (Asteraceae sobre o tumor de Erlich ascítico no camundongo Effect of myriadenolide isolated from Alomia myriadenia (Asteraceae on Ehrlich tumor in its ascitic form
D. Verçosa Júnior
II: 31µg/kg myriadenolide; and group III: 139µg/kg myriadenolide. On the eighth day of the experiment blood profile and protein serum electrophoresis were performed. All ascitic liquid was collected to evaluate the volume and pH; to observe the aspect; to count viable and no viable cells, dark and clear cells, leukocytes and nucleolar organizer regions (NORs. Macro and microscopic exams were performed and bone marrow was aspirated from right and left femurs of each animal to evaluate myeloid:erythroid ratio. It was not observed difference in volume, pH, counts viable and no viable cells in the groups, although group III showed smaller number of viable tumoral cells (17.6 x 10(4 when compared to the group II (27.7 x 10(4 and group I (21.1 x 10(4. The investigation of NORs to evaluate the proliferative capacity of tumoral cells after myriadenolide treatment showed that cells were smaller, uncountable and with diffuse distribution in group I. They were in lower quantity in group III. These results suggest that myriadenolide in dose 139µg/kg (group III delay the tumoral growing and, probably, cell proliferation. The animals of group III showed lower value of total protein (4.7g/dl (P<0.05 when compared to animals from group II (5.3g/dl and group I (5.1g/dl. The values of albumin were similar in all groups (2.6g/dl and total globulin was higher (P<0.05 in group II (2.71g/dl when compared to mean values of group III (2.11g/dl and similar to group I (2.43g/dl. The decrease of total protein in group III occurred by globulin reduction. There was no difference in alpha and beta globulin in the three studied groups, although the immunoglobulins were higher (P<0.05 in group II (1.72g/dl when compared to group III (1.13g/dl. These results suggest the viable number of tumoral cells in group II could cause the increase response of IgM reflecting on the final value of immunoglobulins. In relation to erythrogram and leukogram there was no statistical difference. The myeloid