WorldWideScience

Sample records for berkelium 251

  1. 42 CFR 423.251 - Scope.

    Science.gov (United States)

    2010-10-01

    ... 42 Public Health 3 2010-10-01 2010-10-01 false Scope. 423.251 Section 423.251 Public Health CENTERS FOR MEDICARE & MEDICAID SERVICES, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICARE...; Plan Approval § 423.251 Scope. This section sets forth the requirements and limitations on...

  2. 36 CFR 251.50 - Scope.

    Science.gov (United States)

    2010-07-01

    ... 36 Parks, Forests, and Public Property 2 2010-07-01 2010-07-01 false Scope. 251.50 Section 251.50... § 251.50 Scope. (a) All uses of National Forest System lands, improvements, and resources, except those... routine operation or maintenance activity within the scope of a statutory right-of-way for a...

  3. 37 CFR 1.251 - Unlocatable file.

    Science.gov (United States)

    2010-07-01

    ... 37 Patents, Trademarks, and Copyrights 1 2010-07-01 2010-07-01 false Unlocatable file. 1.251 Section 1.251 Patents, Trademarks, and Copyrights UNITED STATES PATENT AND TRADEMARK OFFICE, DEPARTMENT OF... § 1.251 Unlocatable file. (a) In the event that the Office cannot locate the file of an...

  4. 49 CFR 234.251 - Standby power.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 4 2010-10-01 2010-10-01 false Standby power. 234.251 Section 234.251 Transportation Other Regulations Relating to Transportation (Continued) FEDERAL RAILROAD ADMINISTRATION..., Inspection, and Testing Inspections and Tests § 234.251 Standby power. Standby power shall be tested at...

  5. 7 CFR 251.6 - Distribution plan.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 4 2010-01-01 2010-01-01 false Distribution plan. 251.6 Section 251.6 Agriculture... GENERAL REGULATIONS AND POLICIES-FOOD DISTRIBUTION THE EMERGENCY FOOD ASSISTANCE PROGRAM § 251.6 Distribution plan. Link to an amendment published at 74 FR 62474, Nov. 30, 2009. (a) Contents of the plan....

  6. 40 CFR 25.1 - Introduction.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 1 2010-07-01 2010-07-01 false Introduction. 25.1 Section 25.1 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY GENERAL PUBLIC PARTICIPATION IN PROGRAMS UNDER THE... Introduction. This part sets forth minimum requirements and suggested program elements for public...

  7. 40 CFR 436.251 - Specialized definitions.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 29 2010-07-01 2010-07-01 false Specialized definitions. 436.251 Section 436.251 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS MINERAL MINING AND PROCESSING POINT SOURCE CATEGORY Jade Subcategory §...

  8. 37 CFR 251.11 - Open meetings.

    Science.gov (United States)

    2010-07-01

    ... 37 Patents, Trademarks, and Copyrights 1 2010-07-01 2010-07-01 false Open meetings. 251.11 Section... Copyright Arbitration Royalty Panel Meetings § 251.11 Open meetings. (a) All meetings of a Copyright Arbitration Royalty Panel shall be open to the public, with the exception of meetings that are listed in §...

  9. 37 CFR 251.22 - Public access.

    Science.gov (United States)

    2010-07-01

    ... 37 Patents, Trademarks, and Copyrights 1 2010-07-01 2010-07-01 false Public access. 251.22 Section... ROYALTY PANEL RULES AND PROCEDURES COPYRIGHT ARBITRATION ROYALTY PANEL RULES OF PROCEDURE Public Access to and Inspection of Records § 251.22 Public access. (a) Location of records. All of the...

  10. 40 CFR 421.251 - Specialized definitions.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 28 2010-07-01 2010-07-01 true Specialized definitions. 421.251 Section 421.251 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS NONFERROUS METALS MANUFACTURING POINT SOURCE CATEGORY Primary Precious Metals...

  11. 7 CFR 251.2 - Administration.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 4 2010-01-01 2010-01-01 false Administration. 251.2 Section 251.2 Agriculture... Administration. (a) Food and Nutrition Service. Within the United States Department of Agriculture (the... this section, any information on changes in program administration, including any changes...

  12. 18 CFR 2.51 - [Reserved

    Science.gov (United States)

    2010-04-01

    ... 18 Conservation of Power and Water Resources 1 2010-04-01 2010-04-01 false 2.51 Section 2.51 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY GENERAL RULES GENERAL POLICY AND INTERPRETATIONS Statements of General Policy and Interpretations Under...

  13. 24 CFR 92.251 - Property standards.

    Science.gov (United States)

    2010-04-01

    ... Development HOME INVESTMENT PARTNERSHIPS PROGRAM Project Requirements § 92.251 Property standards. (a) (1..., rehabilitation standards, ordinances, and zoning ordinances at the time of project completion, except as...

  14. 40 CFR 98.251 - Reporting threshold.

    Science.gov (United States)

    2010-07-01

    ...) MANDATORY GREENHOUSE GAS REPORTING Petroleum Refineries § 98.251 Reporting threshold. You must report GHG emissions under this subpart if your facility contains a petroleum refineries process and the facility...

  15. Dicty_cDB: CHC251 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH (Link to library) CHC251 (Link to dictyBase) - - - Contig-U16381-1 - (Link to Original site) CHC2...51F 150 - - - - - - Show CHC251 Library CH (Link to library) Clone ID CHC251 (Link to dicty...iol.tsukuba.ac.jp/CSM/CH/CHC2-C/CHC251Q.Seq.d/ Representative seq. ID - (Link to ...Original site) Representative DNA sequence >CHC251 (CHC251Q) /CSM/CH/CHC2-C/CHC251Q.Seq.d/ CTGTTGGCCTACTGGTA...d/ 186 6e-47 CHC476 (CHC476Q) /CSM/CH/CHC4-D/CHC476Q.Seq.d/ 186 6e-47 CHC251 (CHC251Q) /CSM/CH/CHC2-C/CHC251Q.Seq.d/ 186 6e-47 CHC2

  16. 37 CFR 251.31 - Financial interests.

    Science.gov (United States)

    2010-07-01

    ... Standards of Conduct § 251.31 Financial interests. (a) No selected arbitrator shall have a direct or... sell is not under the control of the selected arbitrator, or (2) Receiving any post-employment benefit... will serve to disqualify the selected arbitrator to the same extent as if they were the...

  17. 27 CFR 25.251 - Authorized removals.

    Science.gov (United States)

    2010-04-01

    ..., DEPARTMENT OF THE TREASURY LIQUORS BEER Removal of Brewer's Yeast and Other Articles § 25.251 Authorized removals. (a) Brewer's yeast. A brewer may remove brewer's yeast, in liquid or solid form containing not... including the words “Brewer's Yeast.” (c) Pipeline. If brewer's yeast is removed by pipeline, the......

  18. 36 CFR 251.97 - Oral presentation.

    Science.gov (United States)

    2010-07-01

    ... 36 Parks, Forests, and Public Property 2 2010-07-01 2010-07-01 false Oral presentation. 251.97... presentation. (a) Purpose. An oral presentation provides an additional opportunity for an appellant, and other..., emphasize, and/or clarify information related to an appeal. Oral presentations are to be conducted in...

  19. 40 CFR 61.251 - Definitions.

    Science.gov (United States)

    2010-07-01

    ... impoundment means any uranium mill tailings impoundment which is licensed to accept additional tailings and is... Operating Mill Tailings § 61.251 Definitions. As used in this subpart, all terms not defined here have the.... (g) Uranium byproduct material or tailings means the waste produced by the extraction...

  20. 36 CFR 251.57 - Rental fees.

    Science.gov (United States)

    2010-07-01

    ... 251.57 Parks, Forests, and Public Property FOREST SERVICE, DEPARTMENT OF AGRICULTURE LAND USES Special... business management principles. (2) Where annual fees of one hundred dollars ($100) or less are assessed... revenue from the authorized use is customer charges; or (2) The holder is a nonprofit association...

  1. 37 CFR 251.3 - Arbitrator lists.

    Science.gov (United States)

    2010-07-01

    ... Organization § 251.3 Arbitrator lists. (a) Any professional arbitration association or organization may submit... types of proceedings in which the person represented clients. (3) A brief description of the educational... on a CARP. (6) Any other information which the professional arbitration association or...

  2. 7 CFR 251.3 - Definitions.

    Science.gov (United States)

    2010-01-01

    ... Regulations of the Department of Agriculture (Continued) FOOD AND NUTRITION SERVICE, DEPARTMENT OF AGRICULTURE GENERAL REGULATIONS AND POLICIES-FOOD DISTRIBUTION THE EMERGENCY FOOD ASSISTANCE PROGRAM § 251.3... children, or child nutrition programs providing food service; (iv) Nutrition projects operating under...

  3. 37 CFR 251.5 - Qualifications of the arbitrators.

    Science.gov (United States)

    2010-07-01

    ... 37 Patents, Trademarks, and Copyrights 1 2010-07-01 2010-07-01 false Qualifications of the arbitrators. 251.5 Section 251.5 Patents, Trademarks, and Copyrights COPYRIGHT OFFICE, LIBRARY OF CONGRESS... PROCEDURE Organization § 251.5 Qualifications of the arbitrators. In order to serve as an arbitrator to...

  4. 27 CFR 25.1 - Production and removal of beer.

    Science.gov (United States)

    2010-04-01

    ... beer. 25.1 Section 25.1 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE BUREAU, DEPARTMENT OF THE TREASURY LIQUORS BEER Scope of Regulations § 25.1 Production and removal of beer. The regulations in this part relate to beer and cereal beverages and cover the location, construction,...

  5. 37 CFR 251.23 - FOIA and Privacy Act.

    Science.gov (United States)

    2010-07-01

    ... 37 Patents, Trademarks, and Copyrights 1 2010-07-01 2010-07-01 false FOIA and Privacy Act. 251.23 Section 251.23 Patents, Trademarks, and Copyrights COPYRIGHT OFFICE, LIBRARY OF CONGRESS COPYRIGHT... Access to and Inspection of Records § 251.23 FOIA and Privacy Act. Freedom of Information Act and...

  6. 46 CFR 153.251 - Independent cargo tanks.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 5 2010-10-01 2010-10-01 false Independent cargo tanks. 153.251 Section 153.251... CARRYING BULK LIQUID, LIQUEFIED GAS, OR COMPRESSED GAS HAZARDOUS MATERIALS Design and Equipment Cargo Tanks § 153.251 Independent cargo tanks. All independent cargo tank must meet § 38.05-10 (a)(1), (b), (d),...

  7. Organic Matter in Space (IAU S251)

    Science.gov (United States)

    Kwok, Sun; Sanford, Scott

    2009-01-01

    Preface; From the local organising committee; Organising committee; Conference participants; Opening address of Symposium 251 C. Cesarsky; Session I. Observations of organic compounds beyond the Solar System William Irvine, Ewine van Dishoeck, Yvonne Pendleton and Hans Olofsson; Session II. Organic compounds within the Solar System Scott Sandford, Ernst Zinner and Dale Cruikshank; Session III. Laboratory analogues of organic compounds in space Max Bernstein and Thomas Henning; Banquet speech; Author index; Object index.

  8. 18 CFR 25.1 - Contents of application.

    Science.gov (United States)

    2010-04-01

    .... 25.1 Section 25.1 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY REGULATIONS UNDER THE FEDERAL POWER ACT APPLICATION FOR VACATION OF WITHDRAWAL AND FOR... reservation effected by the filing of an application for preliminary permit or license, or for a...

  9. 40 CFR 265.251 - Protection from wind.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 25 2010-07-01 2010-07-01 false Protection from wind. 265.251 Section... FACILITIES Waste Piles § 265.251 Protection from wind. The owner or operator of a pile containing hazardous waste which could be subject to dispersal by wind must cover or otherwise manage the pile so that...

  10. Dicty_cDB: VSH251 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available S/VSH2-C/VSH251Q.Seq.d/ Representative seq. ID VSH251F (Link to Original site) Representative...as ID - NBRP ID - dictyBase ID - Link to Contig - Original site URL http://dictycdb.biol.tsukuba.ac.jp/CSM/V

  11. 8 CFR 251.2 - Notification of illegal landings.

    Science.gov (United States)

    2010-01-01

    ... the United States shall inform the immigration officer in charge of the port where the illegal landing... 8 Aliens and Nationality 1 2010-01-01 2010-01-01 false Notification of illegal landings. 251.2 Section 251.2 Aliens and Nationality DEPARTMENT OF HOMELAND SECURITY IMMIGRATION REGULATIONS ARRIVAL...

  12. 14 CFR 125.251 - Required inspection personnel.

    Science.gov (United States)

    2010-01-01

    ... 14 Aeronautics and Space 3 2010-01-01 2010-01-01 false Required inspection personnel. 125.251 Section 125.251 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION... CAPACITY OF 6,000 POUNDS OR MORE; AND RULES GOVERNING PERSONS ON BOARD SUCH AIRCRAFT Maintenance §...

  13. 47 CFR 25.251 - Special requirements for coordination.

    Science.gov (United States)

    2010-10-01

    ... 47 Telecommunication 2 2010-10-01 2010-10-01 false Special requirements for coordination. 25.251... SATELLITE COMMUNICATIONS Technical Standards § 25.251 Special requirements for coordination. (a) The administrative aspects of the coordination process are set forth in § 101.103 of this chapter in the case...

  14. 36 CFR 251.81 - Definitions and terminology.

    Science.gov (United States)

    2010-07-01

    ... 36 Parks, Forests, and Public Property 2 2010-07-01 2010-07-01 false Definitions and terminology. 251.81 Section 251.81 Parks, Forests, and Public Property FOREST SERVICE, DEPARTMENT OF AGRICULTURE... Definitions and terminology. For the purposes of this subpart, the following terms are defined: Appeal....

  15. 37 CFR 251.4 - Arbitrator lists: Objections.

    Science.gov (United States)

    2010-07-01

    ... 37 Patents, Trademarks, and Copyrights 1 2010-07-01 2010-07-01 false Arbitrator lists: Objections... PROCEDURE Organization § 251.4 Arbitrator lists: Objections. (a) In the case of a rate adjustment proceeding... objection with the Librarian of Congress to one or more of the persons contained on the arbitrator list...

  16. 37 CFR 251.8 - Suspension of proceedings.

    Science.gov (United States)

    2010-07-01

    ... selected arbitrator under § 251.6 or to remove and replace a selected arbitrator under subpart D of this... Librarian will take the necessary steps to replace the arbitrator or arbitrators, and upon such replacement... emergency affecting an arbitrator, the Librarian considers a suspension of a proceeding necessary and...

  17. 7 CFR 3550.251 - Property management and disposition.

    Science.gov (United States)

    2010-01-01

    ... property through direct sales, sealed bid, or auction. RHS will follow affirmative fair housing marketing..., DEPARTMENT OF AGRICULTURE DIRECT SINGLE FAMILY HOUSING LOANS AND GRANTS Post-Servicing Actions § 3550.251... properties are reserved for eligible direct or guaranteed single family housing loans under this part or...

  18. 27 CFR 28.251 - Railway express receipts.

    Science.gov (United States)

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2010-04-01 2010-04-01 false Railway express receipts... § 28.251 Railway express receipts. Where the exportation is to a contiguous foreign country and the shipment is by railway express, a receipt issued by the railway express agency may be accepted in lieu...

  19. 37 CFR 251.32 - Financial disclosure statement.

    Science.gov (United States)

    2010-07-01

    ... 37 Patents, Trademarks, and Copyrights 1 2010-07-01 2010-07-01 false Financial disclosure... PROCEDURE Standards of Conduct § 251.32 Financial disclosure statement. (a) Within 45 days of their... shall file with the Librarian an updated confidential financial disclosure form or, if there are...

  20. 37 CFR 251.30 - Basic obligations of arbitrators.

    Science.gov (United States)

    2010-07-01

    ... COPYRIGHT ARBITRATION ROYALTY PANEL RULES AND PROCEDURES COPYRIGHT ARBITRATION ROYALTY PANEL RULES OF... person named in the “arbitration list” published in accordance with § 251.3 of these regulations. (b... ethical and legal principles above private gain. (2) Arbitrators shall not hold financial interests...

  1. 37 CFR 251.64 - Disposition of petition; initiation of arbitration proceeding.

    Science.gov (United States)

    2010-07-01

    ...; initiation of arbitration proceeding. 251.64 Section 251.64 Patents, Trademarks, and Copyrights COPYRIGHT OFFICE, LIBRARY OF CONGRESS COPYRIGHT ARBITRATION ROYALTY PANEL RULES AND PROCEDURES COPYRIGHT ARBITRATION ROYALTY PANEL RULES OF PROCEDURE Rate Adjustment Proceedings § 251.64 Disposition of...

  2. 37 CFR 251.2 - Purpose of Copyright Arbitration Royalty Panels.

    Science.gov (United States)

    2010-07-01

    ... Arbitration Royalty Panels. 251.2 Section 251.2 Patents, Trademarks, and Copyrights COPYRIGHT OFFICE, LIBRARY OF CONGRESS COPYRIGHT ARBITRATION ROYALTY PANEL RULES AND PROCEDURES COPYRIGHT ARBITRATION ROYALTY PANEL RULES OF PROCEDURE Organization § 251.2 Purpose of Copyright Arbitration Royalty Panels....

  3. 37 CFR 251.72 - Declaration of controversy: Initiation of arbitration proceeding.

    Science.gov (United States)

    2010-07-01

    ...: Initiation of arbitration proceeding. 251.72 Section 251.72 Patents, Trademarks, and Copyrights COPYRIGHT OFFICE, LIBRARY OF CONGRESS COPYRIGHT ARBITRATION ROYALTY PANEL RULES AND PROCEDURES COPYRIGHT ARBITRATION ROYALTY PANEL RULES OF PROCEDURE Royalty Fee Distribution Proceedings § 251.72 Declaration...

  4. 37 CFR 251.7 - Actions of Copyright Arbitration Royalty Panels.

    Science.gov (United States)

    2010-07-01

    ... Arbitration Royalty Panels. 251.7 Section 251.7 Patents, Trademarks, and Copyrights COPYRIGHT OFFICE, LIBRARY OF CONGRESS COPYRIGHT ARBITRATION ROYALTY PANEL RULES AND PROCEDURES COPYRIGHT ARBITRATION ROYALTY PANEL RULES OF PROCEDURE Organization § 251.7 Actions of Copyright Arbitration Royalty Panels....

  5. 37 CFR 251.6 - Composition and selection of Copyright Arbitration Royalty Panels.

    Science.gov (United States)

    2010-07-01

    ... Copyright Arbitration Royalty Panels. 251.6 Section 251.6 Patents, Trademarks, and Copyrights COPYRIGHT OFFICE, LIBRARY OF CONGRESS COPYRIGHT ARBITRATION ROYALTY PANEL RULES AND PROCEDURES COPYRIGHT ARBITRATION ROYALTY PANEL RULES OF PROCEDURE Organization § 251.6 Composition and selection of...

  6. 37 CFR 251.54 - Assessment of costs of arbitration panels.

    Science.gov (United States)

    2010-07-01

    ... arbitration panels. 251.54 Section 251.54 Patents, Trademarks, and Copyrights COPYRIGHT OFFICE, LIBRARY OF CONGRESS COPYRIGHT ARBITRATION ROYALTY PANEL RULES AND PROCEDURES COPYRIGHT ARBITRATION ROYALTY PANEL RULES OF PROCEDURE Procedures of Copyright Arbitration Royalty Panels § 251.54 Assessment of costs...

  7. 42 CFR 403.251 - Loss ratio date and time frame provisions.

    Science.gov (United States)

    2010-10-01

    ... 42 Public Health 2 2010-10-01 2010-10-01 false Loss ratio date and time frame provisions. 403.251 Section 403.251 Public Health CENTERS FOR MEDICARE & MEDICAID SERVICES, DEPARTMENT OF HEALTH AND HUMAN... Certification Program: Loss Ratio Provisions § 403.251 Loss ratio date and time frame provisions. (a)...

  8. 37 CFR 251.46 - Conduct of hearings: Role of arbitrators.

    Science.gov (United States)

    2010-07-01

    ... arbitrators. 251.46 Section 251.46 Patents, Trademarks, and Copyrights COPYRIGHT OFFICE, LIBRARY OF CONGRESS... arbitrators. (a) At the opening of a hearing conducted by a Copyright Arbitration Royalty Panel, the chairperson shall announce the subject under consideration. (b) Only the arbitrators of a CARP, or counsel...

  9. 37 CFR 251.36 - Pre-arbitration and post-arbitration employment restrictions.

    Science.gov (United States)

    2010-07-01

    ... 37 Patents, Trademarks, and Copyrights 1 2010-07-01 2010-07-01 false Pre-arbitration and post-arbitration employment restrictions. 251.36 Section 251.36 Patents, Trademarks, and Copyrights COPYRIGHT OFFICE, LIBRARY OF CONGRESS COPYRIGHT ARBITRATION ROYALTY PANEL RULES AND PROCEDURES...

  10. 30 CFR 251.4 - Types of G&G activities that require permits or Notices.

    Science.gov (United States)

    2010-07-01

    ... G&G exploration, including deep stratigraphic tests, for oil, gas, or sulphur resources. If you... 30 Mineral Resources 2 2010-07-01 2010-07-01 false Types of G&G activities that require permits or Notices. 251.4 Section 251.4 Mineral Resources MINERALS MANAGEMENT SERVICE, DEPARTMENT OF THE...

  11. 7 CFR 2.51 - Deputy Under Secretary for Food Safety.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 1 2010-01-01 2010-01-01 false Deputy Under Secretary for Food Safety. 2.51 Section 2... Safety § 2.51 Deputy Under Secretary for Food Safety. Pursuant to § 2.18, and subject to policy guidance... Secretary for Food Safety to the Deputy Under Secretary for Food Safety, to be exercised only during...

  12. TWOS - TIME WARP OPERATING SYSTEM, VERSION 2.5.1

    Science.gov (United States)

    Bellenot, S. F.

    1994-01-01

    The Time Warp Operating System (TWOS) is a special-purpose operating system designed to support parallel discrete-event simulation. TWOS is a complete implementation of the Time Warp mechanism, a distributed protocol for virtual time synchronization based on process rollback and message annihilation. Version 2.5.1 supports simulations and other computations using both virtual time and dynamic load balancing; it does not support general time-sharing or multi-process jobs using conventional message synchronization and communication. The program utilizes the underlying operating system's resources. TWOS runs a single simulation at a time, executing it concurrently on as many processors of a distributed system as are allocated. The simulation needs only to be decomposed into objects (logical processes) that interact through time-stamped messages. TWOS provides transparent synchronization. The user does not have to add any more special logic to aid in synchronization, nor give any synchronization advice, nor even understand much about how the Time Warp mechanism works. The Time Warp Simulator (TWSIM) subdirectory contains a sequential simulation engine that is interface compatible with TWOS. This means that an application designer and programmer who wish to use TWOS can prototype code on TWSIM on a single processor and/or workstation before having to deal with the complexity of working on a distributed system. TWSIM also provides statistics about the application which may be helpful for determining the correctness of an application and for achieving good performance on TWOS. Version 2.5.1 has an updated interface that is not compatible with 2.0. The program's user manual assists the simulation programmer in the design, coding, and implementation of discrete-event simulations running on TWOS. The manual also includes a practical user's guide to the TWOS application benchmark, Colliding Pucks. TWOS supports simulations written in the C programming language. It is designed

  13. 30 CFR 251.13 - Reimbursement for the costs of reproducing data and information and certain processing costs.

    Science.gov (United States)

    2010-07-01

    ... and information and certain processing costs. 251.13 Section 251.13 Mineral Resources MINERALS... third party for the reasonable costs of processing geophysical information (which does not include cost... OUTER CONTINENTAL SHELF § 251.13 Reimbursement for the costs of reproducing data and information...

  14. Effect of Human Cytomegalovirus Infection on Nerve Growth Factor Expression in Human Glioma U251 Cells

    Institute of Scientific and Technical Information of China (English)

    HAI-TAO WANG; BIN WANG; ZHI-JUN LIU; ZHI-QIANG BAI; LING LI; HAI-YAN LIU; DONG-MENG QIAN; ZHI-YONG YAN; XU-XIA SONG

    2009-01-01

    Objectives To explore the change of endogenic nerve growth factor (NGF) expression in human glioma cells infected with human cytomegalovirus (HCMV). Methods U251 cells were cultured in RPMI 1640 culture medium and infected with HCMV AD169 strain in vitro to establish a cell model of viral infection. Morphologic changes of U251 cells were observed under inverted microscope before and after infection with HCMV. Expression of NGF gene and protein of cells was detected by RT-PCR and Western blotting before and after infection with HCMV. Results The cytopathic effects of HCMV-infected cells appeared on day 5 after infection. However, differential NGF expression was evident on day 7. NGF expression was decreased significantly in U251 cells on day 7 after infection in comparison with control group (P<0.05). Conclusion HCMV can down-regulate endogenous NGF levels in human glioma cell line U251.

  15. 29 CFR 779.251 - Goods that have lost their out-of-State identity.

    Science.gov (United States)

    2010-07-01

    ... 29 Labor 3 2010-07-01 2010-07-01 false Goods that have lost their out-of-State identity. 779.251... Coverage Interstate Inflow Test Under Prior Act § 779.251 Goods that have lost their out-of-State identity... been processed or manufactured so as to have lost their identity as out-of-State goods before they...

  16. Efficient enantio- and diastereodivergent synthesis of poison-frog alkaloids 251O and trans-223B.

    Science.gov (United States)

    Toyooka, Naoki; Zhou, Dejun; Nemoto, Hideo; Tezuka, Yasuhiro; Kadota, Shigetoshi; Andriamaharavo, Nirina R; Garraffo, H Martin; Spande, Thomas F; Daly, John W

    2009-09-04

    An efficient and flexible synthesis of poison-frog alkaloids 251O and trans-223B has been achieved by using for both alkaloids an enantiodivergent process starting from the common lactam 1. The relative stereochemistry of 251O and trans-223B was determined to be 7 (R = n-C(7)H(15), R' = n-Pr) and 14 by the present enantioselective synthesis.

  17. AM251 Suppresses Epithelial-Mesenchymal Transition of Renal Tubular Epithelial Cells

    Science.gov (United States)

    Yoshinaga, Tomoyo; Uwabe, Kenichiro; Naito, Shoichi; Higashino, Kenichi; Nakano, Toru; Numata, Yoshito

    2016-01-01

    Epithelial-mesenchymal transition (EMT) of renal tubular epithelial cells is one of the causative mechanisms of kidney fibrosis. In our study, we screened lipophilic compounds using a lipid library including approximately 200 lipids to identify those that suppressed EMT induced by a transforming growth factor (TGF)-β1 stimulus. Initial screening was performed with the immortalized HK-2 renal tubule epithelial cell line. The most promising compounds were further tested in RPTEC primary renal tubule epithelial cells. We found that the synthetic lipid AM251 suppressed two hallmark events associated with EMT, the upregulation of collagen 1A1 (COL1A1) and downregulation of E-cadherin. Though AM251 is known to act as an antagonist for the cannabinoid receptor type 1 (CB1) and an agonist for the G protein-coupled receptor 55 (GRP55), the suppression of EMT by AM251 was not mediated through either receptor. Microarray analyses revealed that AM251 inhibited induction of several EMT transcription factors such as SNAIL1, which is the key inducer of EMT, and the AP-1 transcription factors FOSB and JUNB. Activation of SMAD2/3 and p38 mitogen-activated protein kinase (MAPK) was inhibited by AM251, with greater inhibition of the latter, indicating that AM251 acted upstream of SMAD/p38 MAPK in the TGF-β signaling pathway. Our findings regarding the effects of AM251 on the TGF-β signaling pathway may inform development of a novel therapeutic agent suppressing EMT, thus preventing kidney fibrosis. PMID:27936102

  18. AM251 Suppresses Epithelial-Mesenchymal Transition of Renal Tubular Epithelial Cells.

    Science.gov (United States)

    Yoshinaga, Tomoyo; Uwabe, Kenichiro; Naito, Shoichi; Higashino, Kenichi; Nakano, Toru; Numata, Yoshito; Kihara, Akio

    2016-01-01

    Epithelial-mesenchymal transition (EMT) of renal tubular epithelial cells is one of the causative mechanisms of kidney fibrosis. In our study, we screened lipophilic compounds using a lipid library including approximately 200 lipids to identify those that suppressed EMT induced by a transforming growth factor (TGF)-β1 stimulus. Initial screening was performed with the immortalized HK-2 renal tubule epithelial cell line. The most promising compounds were further tested in RPTEC primary renal tubule epithelial cells. We found that the synthetic lipid AM251 suppressed two hallmark events associated with EMT, the upregulation of collagen 1A1 (COL1A1) and downregulation of E-cadherin. Though AM251 is known to act as an antagonist for the cannabinoid receptor type 1 (CB1) and an agonist for the G protein-coupled receptor 55 (GRP55), the suppression of EMT by AM251 was not mediated through either receptor. Microarray analyses revealed that AM251 inhibited induction of several EMT transcription factors such as SNAIL1, which is the key inducer of EMT, and the AP-1 transcription factors FOSB and JUNB. Activation of SMAD2/3 and p38 mitogen-activated protein kinase (MAPK) was inhibited by AM251, with greater inhibition of the latter, indicating that AM251 acted upstream of SMAD/p38 MAPK in the TGF-β signaling pathway. Our findings regarding the effects of AM251 on the TGF-β signaling pathway may inform development of a novel therapeutic agent suppressing EMT, thus preventing kidney fibrosis.

  19. 我院251例药品不良反应报告分析%Analysis of 251 cases of adverse drug reactions in our hospital report

    Institute of Scientific and Technical Information of China (English)

    唐锦辉

    2013-01-01

      purpose: to understand adverse drug reactions in our hospital (Adverse Drug Reaction) the incidence and relevant factors, provide reference for rational drug use, avoid the occurrence of adverse drug reactions. Methods: 251 cases on our 2011 colection statistics, analysis of ADR reports. Results 251 cases ADR report total involved 16 class drugs, vein drops note is caused ADR of main to drug way (201 cases); antibiotics occurred rate Supreme (142 cases), second is proprietary Chinese medicines (28 cases), and antipyretic analgesia drug (20 cases), and anti-tumor drug (16 cases), and effect blood and hematopoietic system of drug (12 cases); clinical performance main for skin and damage (99 cases), accounted for 39.44%, digestive system of of damage (75 cases) accounted for 29.88%. Conclusion: ADR is related to many factors relevant to clinical ADR monitoring work should be strengthened to reduce or avoid the occurrence of ADR.

  20. 36 CFR 251.103 - Mediation of term grazing permit disputes.

    Science.gov (United States)

    2010-07-01

    ... 36 Parks, Forests, and Public Property 2 2010-07-01 2010-07-01 false Mediation of term grazing... Lands § 251.103 Mediation of term grazing permit disputes. (a) Decisions subject to mediation. In those States with Department of Agriculture certified mediation programs, any holder of a term grazing...

  1. 36 CFR 251.123 - Most directly affected Native Corporation determination.

    Science.gov (United States)

    2010-07-01

    ... 36 Parks, Forests, and Public Property 2 2010-07-01 2010-07-01 false Most directly affected Native..., DEPARTMENT OF AGRICULTURE LAND USES Revenue-Producing Visitor Services in Alaska § 251.123 Most directly... give them an opportunity to submit an application to be considered the Native Corporation most...

  2. 37 CFR 251.56 - Order of the Librarian of Congress.

    Science.gov (United States)

    2010-07-01

    ... 37 Patents, Trademarks, and Copyrights 1 2010-07-01 2010-07-01 false Order of the Librarian of... PROCEDURE Procedures of Copyright Arbitration Royalty Panels § 251.56 Order of the Librarian of Congress. (a... the determination of a panel, the Librarian of Congress shall issue an order accepting the...

  3. 37 CFR 251.53 - Report to the Librarian of Congress.

    Science.gov (United States)

    2010-07-01

    ... 37 Patents, Trademarks, and Copyrights 1 2010-07-01 2010-07-01 false Report to the Librarian of... PROCEDURE Procedures of Copyright Arbitration Royalty Panels § 251.53 Report to the Librarian of Congress... Librarian of Congress a report incorporating its written determination. Such determination shall...

  4. 20 CFR 404.251 - Subsequent entitlement to benefits less than 12 months after entitlement to disability benefits...

    Science.gov (United States)

    2010-04-01

    ... 12 months after entitlement to disability benefits ended. 404.251 Section 404.251 Employees' Benefits SOCIAL SECURITY ADMINISTRATION FEDERAL OLD-AGE, SURVIVORS AND DISABILITY INSURANCE (1950- ) Computing Primary Insurance Amounts Special Computation Rules for People Who Had A Period of Disability §...

  5. 43 CFR 30.251 - What happens if an heir or devisee participates in the killing of the decedent?

    Science.gov (United States)

    2010-10-01

    ... participates in the killing of the decedent? 30.251 Section 30.251 Public Lands: Interior Office of the... if an heir or devisee participates in the killing of the decedent? Any person who knowingly participates, either as a principal or as an accessory before the fact, in the willful and unlawful killing...

  6. 40 CFR 147.251 - EPA-administered program-Class I, III, IV and V wells and Indian lands.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 22 2010-07-01 2010-07-01 false EPA-administered program-Class I, III, IV and V wells and Indian lands. 147.251 Section 147.251 Protection of Environment ENVIRONMENTAL... Indian lands. (a) Contents. The UIC program in the State of California for Class I, III, IV and V...

  7. Isoform of Vascular Endothelial Cell Growth Inhibitor (VEGI72-251) Increases Interleukin-2 Production by Activation of T Lymphocytes

    Institute of Scientific and Technical Information of China (English)

    Jing-Juan YAO; Min ZHANG; Xiao-Hui MIAO; Ping ZHAO; Shi-Ying ZHU; Hui DING; Zhong-Tian QI

    2006-01-01

    The objective of this study is to investigate the characteristics of the recombinant variant of human vascular endothelial cell growth inhibitor, VEGI72-251, and compare its biological activities with that of its prototype VEGI24-174. The recombinant plasmid containing the variant VEGI72-251 gene was constructed and expressed in Escherichia coli. The effects of the expressed VEGI72-251 on cell proliferations were checked in the human umbilical vein endothelial cell line and certain tumor cell lines (ECV304 and B 16). The inhibition of VEGI72-251 on angiogenesis was detected in the chorioallantoic membrane of chick embryos. In comparison with VEGI24-174, the recombinant human VEGI72-251 seems to have no effect on the proliferation of endothelial cells and the angiogenesis of the chorioallantoic membrane in vitro. An enzyme-linked immunosorbent assaybased method was used for the measurement of interleukin-2 (IL-2) production by peripheral blood monocytes (PBMCs) treated with VEGI72-251. PBMCs were pretreated with VEGI72-251 (1.25-12.50 μg/ml) for 24 h in vitro, and the IL-2 concentration in PBMC medium was increased from 354 pg/ml to 1256 pg/ml. It can be concluded that VEGI72-251 is able to increase the level of human IL-2 production by the activation of T lymphocytes. Differing from VEGI24-174 on anti-angiogenesis, VEGI72-251 may serve as an anti-cancer factor through its activation of T lymphocytes.

  8. Safety analysis report for the Heavy-Element Facility (Building 251), Lawrence Livermore National Laboratory

    Energy Technology Data Exchange (ETDEWEB)

    Kvam, D.J.

    1982-10-11

    A comprehensive safety analysis was performed on the Lawrence Livermore National Laboratory's Heavy Element Facility, Building 251. The purpose of the analysis was to evaluate the building and its operations in order to inform LLNL and the Department of Energy of the risks they assume at Building 251. This was done by examining all of the energy sources and matching them with the physical and administrative barriers that control, prevent, or mitigate their hazards. Risk was evaluated for each source under both normal and catastrophic circumstances such as fire, flood, high wind, lighting, earthquake, and criticality. No significant safety deficiencies were found; it is concluded that the operation of the facility presents no unacceptable risk.

  9. Interleukin-8 gene polymorphism –251T>A contributes to Alzheimer's disease susceptibility

    Science.gov (United States)

    Qin, Biyong; Li, Li; Wang, Shanshan; Wu, Jun; Huang, Yulan; Zhou, Ping; Bai, Jiao; Zheng, Yan

    2016-01-01

    Abstract Background: Published association studies have investigated the correlation between interleukin-8 (IL-8) gene polymorphism –251T>A and susceptibility to Alzheimer's disease (AD); however, the results are conflicting. Thus, we conducted the meta-analysis to reassess the effect of IL-8 gene –251T>A variant on the risk of AD. Methods: Relevant studies regarding this association were electronically searched and identified from the PubMed, Embase, Cochrane Library, China National Knowledge Infrastructure, and the Chinese Biomedicine Database. The odds ratios (ORs) with the corresponding 95% confidence intervals (95% CIs) were pooled to calculate the strength of this association. Results: Nine studies with a total of 1406 cases and 2152 controls were included in the meta-analysis. Overall, a significant association of IL-8 gene –251T>A polymorphism with increased risk of AD was observed in several genetic models (allele, A vs T: OR=1.32, 95%CI=1.16-1.50; homozygous, AA vs TT: OR=1.70, 95%CI=1.21–2.21; heterozygous, TA vs TT: OR=1.37, 95%CI=1.12–1.69; recessive, AA vs TA+TT: OR=1.40, 95%CI=1.12–1.75). Similarly, such association was also revealed both in Asian and European populations in the subgroup analysis by ethnicity. Conclusion: The current study suggested that IL-8 gene polymorphism –251T>A may contribute to the susceptibility to AD. PMID:27684880

  10. Cytotoxic activity of allogeneic natural killer cells on U251 glioma cells in vitro.

    Science.gov (United States)

    Guo, Meng; Wu, Tingting; Wan, Lixin

    2016-07-01

    The present study aimed to observe the cytotoxic activity of allogeneic natural killer (NK) cells on U251 glioma cells and to investigate their mechanism of action to establish an effective treatment strategy for neuroglioma. Cell survival curves, colony formation assays and karyotype analysis were performed to investigate the characteristics of U251 glioma cells. The present study demonstrated that natural killer group 2, member D (NKG2D)‑major histocompatibility complex class I‑related chain A/B (MICA/B) interactions contributed to the cytotoxic effect of NK cells on K562 and U251 cells. In antibody‑blocking assays to inhibit NKG2D ligands, the cytotoxic activity was not completely attenuated, which suggested that other signaling pathways contribute to the cytotoxic activity of NK cells on tumor cells in addition to the NKG2D‑mediated activity. The present study identified that the expression levels of NKG2D ligands on the surface of target cells influenced the strength of the NK cell immune response. Furthermore, allogeneic NK cells were observed to kill glioma cells in vitro, and this anticancer activity is associated with the rate of NKG2D expression on the surface of glioma cells.

  11. AM-251 and rimonabant act as direct antagonists at mu-opioid receptors: implications for opioid/cannabinoid interaction studies.

    Science.gov (United States)

    Seely, Kathryn A; Brents, Lisa K; Franks, Lirit N; Rajasekaran, Maheswari; Zimmerman, Sarah M; Fantegrossi, William E; Prather, Paul L

    2012-10-01

    Mu-opioid and CB1-cannabinoid agonists produce analgesia; however, adverse effects limit use of drugs in both classes. Additive or synergistic effects resulting from concurrent administration of low doses of mu- and CB1-agonists may produce analgesia with fewer side effects. Synergism potentially results from interaction between mu-opioid receptors (MORs) and CB1 receptors (CB1Rs). AM-251 and rimonabant are CB1R antagonist/inverse agonists employed to validate opioid-cannabinoid interactions, presumed to act selectively at CB1Rs. Therefore, the potential for direct action of these antagonists at MORs is rarely considered. This study determined if AM-251 and/or rimonabant directly bind and modulate the function of MORs. Surprisingly, AM-251 and rimonabant, but not a third CB1R inverse agonist AM-281, bind with mid-nanomolar affinity to human MORs with a rank order of affinity (K(i)) of AM-251 (251 nM) > rimonabant (652 nM) > AM281 (2135 nM). AM-251 and rimonabant, but not AM-281, also competitively antagonize morphine induced G-protein activation in CHO-hMOR cell homogenates (K(b) = 719 or 1310 nM, respectively). AM-251 and rimonabant block morphine inhibition of cAMP production, while only AM-251 elicits cAMP rebound in CHO-hMOR cells chronically exposed to morphine. AM-251 and rimonabant (10 mg/kg) attenuate morphine analgesia, whereas the same dose of AM-281 produces little effect. Therefore, in addition to high CB1R affinity, AM-251 and rimonabant bind to MORs with mid-nanomolar affinity and at higher doses may affect morphine analgesia via direct antagonism at MORs. Such CB1-independent of these antagonists effects may contribute to reported inconsistencies when CB1/MOR interactions are examined via pharmacological methods in CB1-knockout versus wild-type mice.

  12. siRNA epidermal growth factor receptor silencing in U251 glioma cells

    Institute of Scientific and Technical Information of China (English)

    Chunsheng Kang; Zhiyong Zhang; Zhifan Jia; Qiang Huang; Guangxiu Wang; Mingzhe Qiu; Peiyu Pu

    2008-01-01

    BACKGROUND: Dicer, a large multidomain ribonuclease, is responsible for processing double-stranded RNAs (dsRNAs) to 20-bp-long small interfering RNAs (siRNAs), which act as effectors during RNA interference (RNAi). OBJECTIVE: To observe the efficacy of siRNA cocktails generated by recombinant human Dicer on the down-regulation of epidermal growth factor receptor (EGFR) expression in human glioma cells. DESIGN, TIME AND SETTING: The following in vitro experiment was performed at the Department of Neurosurgery, Tianjin Medical University General Hospital and Laboratory of Neuro-Oncology, Tianjin Neurological Institute. MATERIALS: Mini-RNA isolation kit, human placenta complimentary DNA (cDNA) was produced by Tiangen Biotech (Beijing, China), human glioblastoma U251-MG cells were produced by the Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences. METHODS: A PCR product from the human EGFR, which corresponded to the tyrosine kinase domain of the 3'-end fragment, was used as the T7-promotor for in vitro transcription, siRNA cocktails were generated by in vitro dicing of double stranded RNA. A total of 500, 250 and 125 μg siRNA cocktails were transiently transfected into U251 glioma cells through the use of the GeneSilencer. MAIN OUTCOME MEASURE: Expression of EGFR was detected by real-time PCR. RESULTS: The total PCR product of the human EGFR, corresponding to the tyrosine kinase domain, is approximately 680 bp in length. The PCR transcriptants included GCC leader sequences and a T7 promoter sequence, with a fragment of EGFR cDNA at the center. The T7 promoter was prepared for in vitro transcription of dsRNA. After dicing for 24 hours, the 21-nt siRNA cocktails were verified by 4% agarose gel. The difference between threshold cycle of a sample assay and threshold cycle of the corresponding endogenous reference (△ Ct) among parental U251 cells and cells transfected with different doses of siRNA cocktails were determined to be 3.06, 7.35, and 10

  13. 251例突发性聋预后相关因素的疗效分析%Clinical effective analyses of sudden deafness in 251 cases

    Institute of Scientific and Technical Information of China (English)

    刘少清; 林文敏; 李鸿; 孔喆; 李云英; 杨洪

    2016-01-01

    Objective To investigate the factors related to curative effect and prognosis of sudden deafness (SD). Methods Clinical data of 251 patients (261 ears) with SD were analyzed retrospectively.Analyzed factors included patients' gender,age,visiting time,tinnitus,vertigo,degree of hearing loss and hearing loss curve patterns.SPSS software was applied to single factor analysis (χ2 test). Results 261cases of sudden deafness patients,53cases were cured(20.31%),obviously effective in 27 cases(10.34%),effective in 82cases(31.42%),invalid 99 cases(37.93%),The total effective rate was 62.07%. Statistic analysis showed that the patients' gender and tinnitus had no influence on the curative effect of SD (P>0.05), while the visiting time, degree of hearing loss, patterns of hearing loss curve, age and vertigo were related to it. Satisfactory therapeutic effect could be achieved in patients with treatment received within 7 days after onset, hearing loss of mild to moderate degree, hearing loss curve of ascending or concave pattern and without vertigo. Conclusion In SD, the course of disease, pattern of hearing curve, degree of hearing loss, age and vertigo are correlated with its prognosis. While the gender and tinnitus had no obvious relevance.%目的:探讨突发性耳聋患者的临床疗效及预后相关因素。方法回顾性分析251例(261耳)突发性耳聋患者的临床资料,应用统计学方法对患者的性别、年龄、发病至就诊时间、耳鸣、眩晕、听力损失程度及听力损失曲线类型进行分析,观察其各因素与突发性耳聋近期疗效的相关性。结果251例(261耳)突发性耳聋患者中,痊愈53耳(20.31%),显效27耳(10.34%),有效82耳(31.42%),无效99耳(37.93%),总有效率为62.07%.突发性耳聋的疗效与患者性别、耳鸣无关(P>0.05),与患者就诊时间,听力损失程度及听力损失曲线类型、年龄、伴发眩晕有关;在发病7d内就诊

  14. Induction of apoptosis in glioblastoma cell line U251 of Cinobufacini%华蟾素诱导人胶质瘤细胞U251凋亡的作用机制研究

    Institute of Scientific and Technical Information of China (English)

    赵志远

    2012-01-01

    目的 研究观察华蟾素(Cinobufacini)对体外培养人胶质瘤细胞U251增殖抑制作用,并初步探讨其诱导U251细胞凋亡的分子机制.方法 平皿克隆形成法测定不同浓度华蟾素对U251细胞锚定依赖性生长能力的影响;使用Wester Blot法观察不同浓度华蟾素处理人胶质瘤细胞48小时后,Bcl-2、Bax蛋白表达变化情况.结果 平皿克隆法显示:华蟾素抑制人胶质瘤细胞U251生长,呈剂量依赖性;Wester Blot显示随着药物浓度升高,华蟾素可以逐渐抑制Bcl-2的蛋白表达,活Bax蛋白表达.结论 华蟾素有诱导人胶质瘤细胞U251细胞凋亡的作用,其机制可能Bcl-2/Bax比值的下调,促进细胞凋亡有关.

  15. 技嘉W251U:随身的全能

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    经常需要外出使用笔记本电脑?那么12英寸的技嘉W251U值得你重点考虑,因为除了小巧机身带来的出色便携性,它还是一款在性能、功能和扩展性等各方面都表现出色的优秀产品。W251U采用了完整的迅驰双核平台(NaDa),不但在稳定性和功耗控制方面让人放心,而且Core Duo T2300处理器、512MB DDR2 533内存和80GBSATA硬盘完全能满足除大型3D游戏之外的大部分应用需要。

  16. EFFECTS OF p16INK4 GENE ON CHEMOSENSITIVITY OF HUMAN GLIOMA U251 CELL LINE TO TENIPOSIDE

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    Objective: To determine the effects on the cell growth, tumorigenicity and chemosensitivity of p16/CDK4I in human glioma. Methods: p16 gene was transfected into U251 cells by lipofectin. Expression of exogenous p16 gene was confirmed by immunohistochemistry and Northern blot. The effects of exogenous p16 gene on the growth and chemosensitivity to teniposide were examined. Results: Expression of exogenous p16 gene inhibited the growth dramatically in vitro. G1 arrest of tumor cells was observed. However, wt p16-positive U251 was less sensitive than control cell lines and the number of apoptotic cells after chemotherapy was reduced. Conclusion: The expression of exogenous p16 gene could inhibit the growth of glioma. On the other hand, the chemosensitivity to teniposide of p16-positive U251 was decreased.

  17. Downregulation of ROCK2 through Nanocomplex Sensitizes the Cytotoxic Effect of Temozolomide in U251 Glioma Cells

    OpenAIRE

    Xiaojun Wen; Amin Huang; Zhonglin Liu; Yunyun Liu; Jingyang Hu; Jun Liu; Xintao Shuai

    2014-01-01

    OBJECTIVE: Rho-associated coiled-coil kinase 2 (ROCK2) is an attractive therapeutic target because it is overexpressed in many malignancies, including glioma. Therefore, we designed the current study to determine whether the downregulation of ROCK2 would sensitize the cytotoxic effect of temozolomide (TMZ) in U251 cells. METHODS: Glycol-polyethyleneimine (PEG-PEI) was used to deliver siROCK2 to U251 cells, and the physical characteristics of the PEG-PEI/siROCK2 complex (referred to as the siR...

  18. M251S型燃气轮机的技术特性及结构特点

    Institute of Scientific and Technical Information of China (English)

    施彧翰; 陆汉军

    2007-01-01

    就中小型钢厂CCPP用M251S型燃气轮机,介绍了该机的燃烧器、压气机、除尘和防泄漏等作为BFG用燃气轮机的特有技术特性。并着重分析TM251S型燃气轮机主要结构压气机、透平及其冷却系统、燃烧室的工作原理和特点。

  19. Long-term repeated biodesulfurization by immobilized Rhodococcus erythropolis KA2-5-1 cells

    Energy Technology Data Exchange (ETDEWEB)

    Naito, M.; Kawamoto, T.; Tanaka, A. [Kyoto Univ., Yoshida (Japan). Dept. of Synthetic Chemistry and Biological Chemistry; Fujino, K.; Kobayashi, M.; Maruhashi, K. [Advanced Technology and Research Inst., Petroleum Energy Center, Shizuoka (Japan)

    2001-07-01

    In this study, biodesulfurization (BDS) was carried out using immobilized Rhodococcus erythropolis KA2-5-1 in n-tetradecane containing dibenzothiophene (DBT) as a model oil (n-tetradecane/immobilized cell biphasic system). The cells were immobilized by entrapping them with calcium alginate, agar, photo-crosslinkable resin prepolymers (ENT-4000 and ENTP-4000), and urethane prepolymers (PU-3 and PU-6); and it was found that ENT-4000-immobilized cells had the highest DBT desulfurization activity in the model oil system without leakage of cells from the support. Furthermore, ENT-4000-immobilized cells could catalyze BDS repeatedly in this system for more than 900 h with reactivation; and recovery of both the biocatalyst and the desulfurized model oil was easy. This study would give a solution to the problems in BDS, such as the troublesome process of recovering desulfurized oil and the short life of BDS biocatalysts. (orig.)

  20. Evaluation of potential regulatory function of breast cancer risk locus at 6q25.1.

    Science.gov (United States)

    Sun, Yaqiong; Ye, Chuanzhong; Guo, Xingyi; Wen, Wanqing; Long, Jirong; Gao, Yu-Tang; Shu, Xiao Ou; Zheng, Wei; Cai, Qiuyin

    2016-02-01

    In a genome-wide association study conducted among Chinese women, we identified the single nucleotide polymorphism (SNP) rs2046210 at 6q25.1 for breast cancer risk. To explore a potential regulatory role for this risk locus, we measured expression levels of nine genes at the locus in breast cancer tissue and adjacent normal tissue samples obtained from 67 patients recruited in the Shanghai Breast Cancer Study. We found that rs2046210 had a statistically significant association with the expression levels of the AKAP12 and ESR1 genes in adjacent normal breast tissues. Women who carry the AA/AG risk genotypes had higher expressions of these two genes compared to those who carry G/G genotypes (P = 0.02 and 0.04 for the AKAP12 and ESR1, respectively). However, no significant differences of SNP rs2046210 with gene expression levels were found in tumor tissues. In The Cancer Genome Atlas samples, the AA/AG risk genotypes of SNP rs2046210 were associated with a significantly higher expression level of the AKAP12 gene and a lower level of the ESR1 gene in tumor tissue. Functional analysis using ENCODE data revealed that SNP rs7763637, which is in strong linkage disequilibrium with SNP rs2046210, is likely a potential functional variant, regulating the AKAP12 gene. Taken together, these results from our study suggest that the association between the 6q25.1 locus and breast cancer risk may be mediated through SNPs that regulate expressions of the AKAP12 gene.

  1. Tumstatin transfected into human glioma cell line U251 represses tumor growth by inhibiting angiogenesis

    Institute of Scientific and Technical Information of China (English)

    YE Hong-xing; YAO Yu; JIANG Xin-jun; YUAN Xian-rui

    2013-01-01

    Background Angiogenesis is a prerequisite for tumor growth and plays an important role in rapidly growing tumors,such as malignant gliomas.A variety of factors controlling the angiogenic balance have been described,and among these,the endogenous inhibitor of angiogenesis,tumstatin,has drawn considerable attention.The current study investigated whether expression of tumstatin by glioma cells could alter this balance and prevent tumor formation.Methods We engineered stable transfectants from human glioma cell line U251 to constitutively secrete a human tumstatin protein with c-myc and polyhistidine tags.Production and secretion of the tumstatin-c-myc-His fusion protein by tumstatin-transfected cells were confirmed by Western blotting analysis.In the present study,we identify the anti-angiogenic capacity of tumstatin using several in vitro and in vivo assays.Student's t-test and one-way analysis of variance (ANOVA) test were used to determine the statistical significance in this study.Results The tumstatin transfectants and control transfectants (stably transfected with a control plasmid) had similar in vitro growth rates compared to their parental cell lines.However,the conditioned medium from the tumstatin transfected tumor cells significantly inhibits proliferation and causes apoptosis of endothelial cells.It also inhibits tube formation of endothelial cells on Matrigel.Examination of armpit tumors arising from cells overexpressing tumstatin repress the growth of tumor,accompanying the decreased density of CD31 positive vessels in tumors ((5.62±1.32)/HP),compared to the control-transfectants group ((23.84+1.71)/HP) and wild type U251 glioma cells group ((29.33+4.45)/HP).Conclusion Anti-angiogenic gene therapy using human tumstatin gene may be an effective strategy for the treatment of glioma.

  2. 白细胞介素-8-251A/T多态性与宫颈癌的相关性%Association between -251A/T polymorphism of interleukin-8 gene and cervical cancer

    Institute of Scientific and Technical Information of China (English)

    曹党恩; 贺国洋; 鲁霞

    2011-01-01

    Objective To investigate the association between interleukin-8(IL-8) -251A/T polymorphism and cervical cancer. Methods The polymorphism of IL-8 -251A/T were analyzed in 219 patients with cervical cancer (cervical cancer group) and 295 healthy person( control group) by polymerase chain reaction restriction fragment length polymorphism. Results The genetypes of TT, AT and AA were present in both cervical cancer group and control group for IL-8 -251 A/T gene polymorphism. The frequency of each genetype was 31. 1% (TT) ,49. 3% (AT) and 19. 6% ( AA) ,and frequency of each allele was 44.3%(A) and55.7%(T) in cervical cancer group. The frequency of each genetype was 42.7% (TT) ,45. 1% (AT) and 12.2% ( AA) ,and frequency of each allele was 34. 7% (A) and 65. 3% (T) in control group. There were significant differences in genetype and allele frequencies of the IL-8 -251 A/T polymorphism between two groups (χ2 =0.953,572.428; P - 0.09,0.000). Conclusion IL-8 -251 A/T polymorphism may be associated with cervical cancer.%目的 探讨白细胞介素-8(IL-8)-251A/T多态性及mRNA与宫颈癌发病的相关性.方法 采用聚合酶链反应-限制性片段长度多态性方法检测219例宫颈癌患者(宫颈癌组)和295例健康对照者(对照组)IL-8-251A/T多态性.结果 宫颈癌组和对照组均发现IL-8-251A/T位点基因多态性,均可见TT、AT和AA基因型;宫颈癌组TT、AT、AA基因型频率分别为31.1%、49.3%和19.6%,A、T等位基因频率分别为44.3%和55.7%;对照组TT、AT、AA基因型频率分别为42.7%、45.1%和12.2%,T、A等位基因频率分别为65.3%和34.7%;宫颈癌组IL-8-251A/T基因型及等位基因频率与对照组比较,差别有统计学意义(x2=0.953,572.428;P=0.009,0.000).结论 IL-8-251A/T多态性与宫颈癌的发病具有相关性.

  3. Effects of temozolomide chemotherapy on CD133+ cells in glioma U251%替莫唑胺化疗对胶质瘤U251中CD133+细胞的影响

    Institute of Scientific and Technical Information of China (English)

    杨伟现; 向定朝; 王存祖; 周月鹏; 欧阳琦; 张志坚; 许慧中; 陆晓峰

    2012-01-01

    目的 探讨化疗耐药与肿瘤干细胞之间的关系.方法 分析替莫唑胺(TMZ)作用前后胶质瘤U251中CD133蛋白表达及阳性细胞数量的变化.通过MTT实验评估U251细胞对TMZ的敏感性.利用免疫荧光染色和Western blot检测TMZ作用前后U251细胞中CD133、巢蛋白(nestin)、神经元特异性烯醇化酶(NSE)、神经胶质细胞纤维酸性蛋白(GFAP)等蛋白表达.结果 U251细胞中未加入TMZ组的CD133+细胞比例约为0.060±0.003,明显低于加入TMZ组的0.337±0.012(P<0.05).在TMZ作用后,CD133和nestin表达明显增加,而GFAP和NSE表达明显减少(P<0.05).结论 胶质瘤U251细胞株中存在CD133+细胞,大部分具有脑肿瘤干细胞特性;在TMZ作用后,这类细胞比例增加,提示其与化疗耐药有关.%Objective To explore the relationship between chemotherapy resistance and cancer stem cells. Methods The changes of the number and protein expression were analyzed before and after the CD133+ cells in glioma U251 were intervented by temozolomide(TMZ). The expressions of CD133,nestin,neuron-specific enolase(NSE),glial fibrillary acidic protein(GFAP) and other proteins in glioma U251 cells were detected by immunofluorescence and Western blot. Results U251 cells in the TMZ group did not join The proportion of CD133+ cells was significantly greater in the U251 cells with TMZ intervention than that without(0. 337±0.012 vs. 0. 060 + 0.003) (P<0. 05). After TMZ intervention, the proportion of nestin-positive cells in the CD133+ cells were increased,but GFAP and NSE-positive cells were decreased(P<0. 05). Conclusion There CD133+cells in the U251 glioma cell lines,majority of which have the properties of brain tumor stem cells. After TMZ intervention, an increased proportion of the CD133+ cells suggests its relationship with chemotherapy-related drug resistance.

  4. 30 CFR 251.12 - Submission, inspection, and selection of geophysical data and information collected under a...

    Science.gov (United States)

    2010-07-01

    ... geophysical data and information collected under a permit and processed by permittees or third parties. 251.12..., and selection of geophysical data and information collected under a permit and processed by permittees or third parties. (a) Availability of geophysical data and information collected under a permit....

  5. 30 CFR 251.11 - Submission, inspection, and selection of geological data and information collected under a permit...

    Science.gov (United States)

    2010-07-01

    ... geological data and information collected under a permit and processed by permittees or third parties. 251.11..., and selection of geological data and information collected under a permit and processed by permittees or third parties. (a) Availability of geological data and information collected under a permit....

  6. 36 CFR 251.19 - Exercise of water rights reserved by the grantor of lands conveyed to the United States.

    Science.gov (United States)

    2010-07-01

    ... 36 Parks, Forests, and Public Property 2 2010-07-01 2010-07-01 false Exercise of water rights... Grantors § 251.19 Exercise of water rights reserved by the grantor of lands conveyed to the United States. This section governs the exercise of water and related rights reserved by the grantor of lands...

  7. 48 CFR 252.251-7001 - Use of Interagency Fleet Management System (IFMS) vehicles and related services.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 3 2010-10-01 2010-10-01 false Use of Interagency Fleet... Fleet Management System (IFMS) vehicles and related services. As prescribed in 251.205, use the following clause: Use of Interagency Fleet Management System (IFMS) Vehicles and Related Services (DEC...

  8. 30 CFR 250.251 - If I propose activities in the Alaska OCS Region, what planning information must accompany the DPP?

    Science.gov (United States)

    2010-07-01

    ... activities in the Alaska OCS Region, the following planning information must accompany your DPP: (a... 30 Mineral Resources 2 2010-07-01 2010-07-01 false If I propose activities in the Alaska OCS Region, what planning information must accompany the DPP? 250.251 Section 250.251 Mineral...

  9. Risk modification of colorectal cancer susceptibility by interleukin-8-251T>A polymorphism in Malaysians

    Institute of Scientific and Technical Information of China (English)

    Mohd Aminudin Mustapha; Siti Nurfatimah Mohd Shahpudin; Ahmad Aizat Abdul Aziz; Ravindran Ankathil

    2012-01-01

    AIM:To investigate the allele and genotype frequencies and associated risk of interleukin (IL)-8-251T>A polymorphism on colorectal cancer (CRC) susceptibility risk.METHODS:Peripheral blood samples of 255 normal controls and 255 clinically and histopathologically confirmed CRC patients were genotyped for IL-8-251T>A polymorphism employing allele-specific polymerase chain reaction.The relative association of variant allele and genotypes with CRC susceptibility risk was determined by calculating the odds ratios (ORs).Corresponding x2 tests on the CRC patients and controls were carried out and 95% confidence intervals (CIs) were determined using Fisher's exact test.The allele frequencies and its risk association were calculated using FAMHAP,haplotype association analysis software.RESULTS:On comparing the frequencies of genotypes of patients and controls,the homozygous variant AA was significantly higher in CRC patients (P =0.002)compared to controls.Investigation on the association of the polymorphic genotypes with CRC susceptibility risk,showed that the homozygous variant IL-8-251AA had a significantly increased risk with OR 3.600 (95%CI:1.550-8.481,P =0.001).In the case of allele frequencies,variant allele A of IL-8-251 showed a significantly increased risk of CRC predisposition with OR 1.32(95% CI:1.03-1.69,P =0.003).CONCLUSION:Variant allele and genotype of IL-8 (-251T>A) was significantly associated with CRC susceptibility risk and could be considered as a high-risk variant for CRC predisposition.

  10. 过表达TAP1上调人胶质瘤细胞株U251 HLA-I的表达%Overexpression of TAP1 up-regulates HLA-I in human glioma U251 cells

    Institute of Scientific and Technical Information of China (English)

    欧阳乐平; 张善义; 李军亮; 许新科; 翁胤仑; 郑眉光; 王圣文; 李方成

    2013-01-01

    AIM: To investigate the relationship between the overexpression of transporter associated with antigen processing 1 (TAP1) and the human leukocyte antigen I (HLA-I). METHODS: The full length of TAP1 gene was obtained from the cDNA library. The lentiviral vector pSIN-EF2-IRES-GFP-puro was digested by BamH I and EcoR I, and the full length of TAP1 gene was inserted into the vector by T4 DNA ligase. Subsequently, the recombinant plasmid was transformed into Escheriehia coli DH5α cells and the correct transformant was selected. The recombinant plasmid and the Lenti-X HTX packaging mixture were co-transfected into 293T cells, and the virus particle was acquired. Human glioma U251 cells were transfected with the lentivirus. The expression of TAP1 and HLA-I was determined by real-time fluorescence quantitative PCR, Western blotting and flow cytometric analysis. RESULTS; TAP1 gene was successfully transfected into the U251 cells and stably expressed in the cell line. The expression of TAP1 in U251 cells at mRNA and protein levels increased by ( 8. 73 ± 1. 07) and (11. 71 ± 0. 83 ) folds, respectively. As a result, the mRNA expression of HLA-A, HLA-B, HLA-C (heavy chain) and β2-microglobulin (light chain) was up-regulated by (3. 51 ±0. 36) , (4. 78 ±0. 85) , (2.94 ±0.28) and (3. 23 ±0. 24) folds, respectively. The protein expression of HLA-I also increased to (3.14 ±0. 53) fold. The surface expression of HLA-I on the U251 cells transfected with TAP1 gene was largely enhanced as well. CONCLUSION : Overexpression of TAP1 up-regulates the expression of HLA-I. TAP1 plays an important role in HLA-I pro-cessing pathway.%目的:探讨抗原提呈相关转运蛋白1(TAPl)过表达对人胶质瘤细胞株U251人类白细胞抗原I(HLA-I)表达的影响.方法:培养U251细胞,构建慢病毒载体并转染U251细胞,获得稳定高表达TAP1的细胞株并设立转染空载体对照组,分别收集U251细胞组、空载体对照组和TAPl基因转染细胞组

  11. Quantitative Proteomic Profiling of Tachyplesin I Targets in U251 Gliomaspheres

    Directory of Open Access Journals (Sweden)

    Xuan Li

    2017-01-01

    Full Text Available Tachyplesin I is a cationic peptide isolated from hemocytes of the horseshoe crab and its anti-tumor activity has been demonstrated in several tumor cells. However, there is limited information providing the global effects and mechanisms of tachyplesin I on glioblastoma multiforme (GBM. Here, by using two complementary proteomic strategies (2D-DIGE and dimethyl isotope labeling-based shotgun proteomics, we explored the effect of tachyplesin I on the proteome of gliomaspheres, a three-dimensional growth model formed by a GBM cell line U251. In total, the expression levels of 192 proteins were found to be significantly altered by tachyplesin I treatment. Gene ontology (GO analysis revealed that many of them were cytoskeleton proteins and lysosomal acid hydrolases, and the mostly altered biological process was related to cellular metabolism, especially glycolysis. Moreover, we built protein–protein interaction network of these proteins and suggested the important role of DNA topoisomerase 2-alpha (TOP2A in the signal-transduction cascade of tachyplesin I. In conclusion, we propose that tachyplesin I might down-regulate cathepsins in lysosomes and up-regulate TOP2A to inhibit migration and promote apoptosis in glioma, thus contribute to its anti-tumor function. Our results suggest tachyplesin I is a potential candidate for treatment of glioma.

  12. Nematode assemblages from the Kandalaksha Depression (White Sea, 251-288 m water depth)

    Science.gov (United States)

    Miljutin, Dmitry M.; Miljutina, Maria A.; Tchesunov, Alexei V.; Mokievsky, Vadim O.

    2014-03-01

    The shallow-water nematodes of the White Sea are relatively well studied; however, information on the nematode fauna inhabiting the deepest part of this sea is very scarce. The composition of the nematode assemblages (at species and genus level) was studied in samples collected during four sampling occasions in the deepest part of the Kandalaksha Depression (the White Sea) in July 1998, October 1998, May 1999, and November 1999. Samples were collected from a depth of 251-288 m with the aid of a multicorer. In total, 59 nematode morphotypes belonging to 37 genera and 18 families were distinguished. The genera Sabatieria and Filipjeva dominated at all stations, followed by Aponema, Desmoscolex, and Quadricoma. The composition of the dominant genera can be considered typical for this depth range in temperate and Arctic waters, although Filipjeva and Aponema were among the dominant genera for the first time. The most abundant species were Sabatieria ornata, Aponema bathyalis, and Filipjeva filipjevi. In general, diversity of the nematode assemblages was lower than in the temperate and Arctic continental shelf and slope with reduced evenness and species richness. The evenness of nematode assemblages and other diversity indices decreased with increasing sediment depth. Based on the valid species and genera recorded, the nematode fauna of the Kandalaksha Depression showed a higher resemblance to that found in the shallow waters of Kandalaksha Bay.

  13. Downregulation of ROCK2 through nanocomplex sensitizes the cytotoxic effect of temozolomide in U251 glioma cells.

    Directory of Open Access Journals (Sweden)

    Xiaojun Wen

    Full Text Available OBJECTIVE: Rho-associated coiled-coil kinase 2 (ROCK2 is an attractive therapeutic target because it is overexpressed in many malignancies, including glioma. Therefore, we designed the current study to determine whether the downregulation of ROCK2 would sensitize the cytotoxic effect of temozolomide (TMZ in U251 cells. METHODS: Glycol-polyethyleneimine (PEG-PEI was used to deliver siROCK2 to U251 cells, and the physical characteristics of the PEG-PEI/siROCK2 complex (referred to as the siROCK2 complex were investigated. The transfection efficiency and cell uptake were determined by flow cytometry (FCM and confocal laser microscopy (CLSM, respectively. U251 cells were then treated with 100 μM TMZ, siROCK2 complexes or their combination. The apoptosis rate and cell migration were measured by FCM and wound-healing assay, respectively. The levels of Bax, Bcl-2, cleaved caspase-3, MMP-2, and MMP-9 were detected to analyze the degrees of apoptosis and migration. RESULTS: Our results revealed that the characteristics of the siROCK2 complexes depended closely on the N/P ratios. PEG-PEI served as a good vector for siROCK2 and exhibited low cytotoxicity toward U251 cells. The CLSM assay showed that the siROCK2 complexes were successfully uptaken and that both the protein and mRNA levels of ROCK2 were significantly suppressed. Furthermore, the combination treatment induced a higher apoptosis rate and markedly increased the gap distance of U251 cells in the wound-healing assay. Levels of the proapoptotic proteins Bax and cleaved caspase-3 were significantly increased, whereas levels of the antiapoptotic protein Bcl-2 and the migration-related proteins MMP-2 and MMP-9 were significantly reduced by the combination treatment compared with either treatment alone. CONCLUSIONS: In conclusion, our results demonstrate that the combination of TMZ and siROCK2 effectively induces apoptosis and inhibits the migration of U251 cells. Therefore, the combination of TMZ

  14. 替莫唑胺对人胶质瘤细胞系U251细胞miR125b的影响%Temozolomide in glioblastoma cells U251 affect miR-125b expression

    Institute of Scientific and Technical Information of China (English)

    程峰; 石磊; 贡伟一; 刘华; 潘天鸿

    2014-01-01

    目的:探讨替莫唑胺( TMZ)对人胶质瘤细胞系U251细胞的miRNA125b的表达影响。方法将人胶质瘤细胞系U251细胞分成空白对照组、TMZ组,TMZ组设10、25、50、100、200、400、600μmol/L组。各组分别作用于24、48、72 h后进行实时定量PCR( Real-time PCR)检测miRNA-125b的表达水平;用MTT比色法检测细胞活力;流式细胞仪检测细胞周期和凋亡率。结果荧光定量PCR检测结果显示:替莫唑胺作用24 h后, miRNA125b表达变化不明显;48 h后对miR-125b表现出抑制作用, miR-125b对U251细胞的抑制率呈良好的时间-剂量效应关系,其Ic 50为76μmol,初始抑制浓度为50μmol;流式细胞仪检测显示,U251细胞经TMZ作用后出现G2/M期阻滞,但促凋亡作用并不显著。结论在TMZ对胶质瘤细胞治疗过程中,hsa-miR-125b的表达随着治疗剂量而被抑制,miR-125b可以作为TMZ在用药过程中效果的检测以及抗药性反应的参考依据。%Objective To investigate the temozolomide(TMZ) affect on the expression of hsa-miR-125b human neureblastoma cell line U251 cells.Method U251 cells we treated 7 different concentrations of TMZ (10、25、50、100、200、400、600 μmol/L) 72 h, hsa-miR-125 expression level was identified by quantitve real time PCR ( qRT-PCR) .Cell viability was accessed by using colorimotrlc MTT assay Cell cycle progression and apoptosis ratio are measured by using flow cytometry .Results After TMZ affect 24h, the changes of miRNA-125b expression were unconspic-uous, and after 48h, the expression of miRNA-125b was inhibited.The IC50 of TMZ Was 76 mol/L.The relationship between the expression level of miR-125b and the inhibition rate of U251 cells was a perfect time to dose response . There were significant difference between the control group and the groups over 50 mol/L(P<0.01). Flow cytometry showed that U251 cells responded to TMZ by undergoing G2/M arrest and very few cells show apoptosis

  15. Structural analyses of the storage container for heavy element facility, building-251

    Energy Technology Data Exchange (ETDEWEB)

    Ng, D S

    1999-01-01

    The Heavy Element Facility, Building 251, contains a series of underground storage vaults which are used for long term storage of nuclear materials. A storage rack with shelves is suspended from the top of each storage vault. The stainless steel containers enclosing the nuclear materials are stored on the shelves. A Hazard & Accident assessment analyzed the vulnerability of this storage system to assaults resulting from natural phenomena and accidents within the building. The assessment considered all racks and their containers to be stored underground and secured in their static, long-term configuration. Moving beyond the static, long-term hazard assessment, the structural analyses were performed to evaluate the storage container against a rare, short duration event. An accidental free drop of a container may occur in a combination of two events: a rare, short-duration earthquake concurrent with an operation of raising the storage rack to a maximum height that the crane is capable of. This hypothetical free drop may occur only to the container in the uppermost shelf of the storage rack. The analyses were the structural evaluation of the storage container to determine the material containment integrity of the storage container after the accident. The evaluation was performed simulating a free drop from the storage rack, with a maximum load in the container, striking/an unyielding surface in the worst orientation. The analyses revealed that, in the very unlikely event of a container drop, the integrity of the hermetic seal of the storage container could be compromised due to plastic deformation of the lid and mating flange. Simple engineering and administrative controls can prevent that from occurring.

  16. Mechanism of thalidomide to enhance cytotoxicity of temozolomide in U251-MG glioma cells in vitro

    Institute of Scientific and Technical Information of China (English)

    GAO Song; YANG Xue-jun; ZHANG Wen-gao; JI Yan-wei; PAN Qiang

    2009-01-01

    Background Glioma is the most common primary brain tumor with poor prognosis. Temozolomide has been used with thalidomide to treat gliomas. We investigated the synergistic mechanism of these two drugs in vitro.Methods Human malignant glioma cells U251-MG were cultured and assigned to four groups with different treatments for 3 days: temozolomide group (100 pmol/L), thalidomide group (100 pg/L), temozolomide (100 IJmol/L) plus thalidomide group (100 pg/L) and control group. MTT assay was applied to evaluate the cell viability. Cell cycle was analyzed by flow cytometry. The ultra-structural features of autophagosomes were observed with electron microscope. Acridine orange and monodansylcadavedne were adopted to label autophagosomes and flow cytometry was applied for quantification of autophagosomes. The expression of autophagy-associated protein was detected by Western blotting.Results Proliferation of tumor cell was obviously suppressed by temozolomide with thalidomide treatment than by either drug used alone (P=-0.000 for each day). The combination treatment induced cell cycle arrest at G0/G1 phase.Typical autophagic ultra-structural character was found after the combined treatment. Thalidomide promoted the autophagy induced by temozolomide. The autophagy-associated proteins - microtubule associated protein 1 light chain 3 (MAPILC3) and Beclinl were more significantly up-regulated by the combined treatment than temozolomide used alone (MAP1LC3, P=-0.000; Beclinl, P=-0.004). The expression level of phosphatase and tensin homolog deleted on chromosome ten (PTEN), which promoted autophagy by suppressing PI3K/Akt/mTOR signaling pathway, was elevated by thalidomide (thalidomide group: P=-0.000; combined group: P=0.002).Conclusions Thalidomide enhances the cytotoxicity of temozolomide by promoting the autophagy induced by temozolomide. Contributing to the up-regulation of PTEN by thalidomide, the expression of autophagy associated protein-MAP1LC3 and Beclinl was enhanced

  17. Preparation of iodine-123 labeled AM251: a potential SPECT radioligand for the brain cannabinoid CB1 receptor

    Energy Technology Data Exchange (ETDEWEB)

    Lan, Ruoxi; Makriyannis, Alexandros [Connecticut Univ., Molecular and Cell Biology Dept., Storrs, CT (United States); Gatley, S.J. [Brookhaven National Lab., Medical Dept., Upton, NY (United States)

    1996-10-01

    We report the synthesis and labeling with iodine-123 of N-(piperidin-1-yl)-5-(4-iodophenyl)-1-(2, 4-dichlorophenyl)-4-methyl-1H-pyrazole-3-carboxamide (AM251). This compound is an analog of the recently described cannabinoid receptor antagonist, SR141716A, in which a 4-chlorophenyl group is replaced by 4-iodophenyl. Labeling in good yield (62%) and radiochemical purity (> 95%), and high specific activity (> 2500 Ci/mmol) was achieved by an iododestannylation reaction using the tributyltin precursor, no carrier added I-123 iodide, and chloramine-T. (author).

  18. Starvation after Cobalt-60 γ-Ray Radiation Enhances Metastasis in U251 Glioma Cells by Regulating the Transcription Factor SP1.

    Science.gov (United States)

    Zhao, Tuo; Wang, Hailong; Ma, Hong; Wang, Hao; Chen, Bo; Deng, Yulin

    2016-04-05

    Radiation is of clinical importance during glioma therapy; however, vasculature damage is observed over the treatment course. This type of tissue damage might lead to starvation conditions, affecting tumor metastasis. To test this possibility, we compared starvation conditions in conjunction with radiation treatment to monitor metastatic ability in the U251 glioma cell line. Transcriptome, western blot, and immunofluorescence analyses were used to measure the RNA and protein expression changes of the U251 cells after various treatments. We found that starvation combined with radiation treatment yielded the most significant expression changes in metastasis-related factors compared to that in the control groups. In addition, a metastasis assay was used to directly measure the metastatic ability of the treated cells, which confirmed that the U251 cells treated with starvation combined with radiation possessed the highest metastatic ability. Furthermore, bioinformatics analysis demonstrated that SP1 represented a common transcription factor associated with changes in metastasis-related factors. Blocking SP1 activity by an inhibitor suppressed the starvation-plus-radiation treatment-mediated enhancement of U251 cell metastasis. Our study provides the first evidence that starvation caused by radiation might play a significant role in enhancing the ability of the glioma cell line U251 to metastasize via regulation of the transcription factor SP1.

  19. Starvation after Cobalt-60 γ-Ray Radiation Enhances Metastasis in U251 Glioma Cells by Regulating the Transcription Factor SP1

    Directory of Open Access Journals (Sweden)

    Tuo Zhao

    2016-04-01

    Full Text Available Radiation is of clinical importance during glioma therapy; however, vasculature damage is observed over the treatment course. This type of tissue damage might lead to starvation conditions, affecting tumor metastasis. To test this possibility, we compared starvation conditions in conjunction with radiation treatment to monitor metastatic ability in the U251 glioma cell line. Transcriptome, western blot, and immunofluorescence analyses were used to measure the RNA and protein expression changes of the U251 cells after various treatments. We found that starvation combined with radiation treatment yielded the most significant expression changes in metastasis-related factors compared to that in the control groups. In addition, a metastasis assay was used to directly measure the metastatic ability of the treated cells, which confirmed that the U251 cells treated with starvation combined with radiation possessed the highest metastatic ability. Furthermore, bioinformatics analysis demonstrated that SP1 represented a common transcription factor associated with changes in metastasis-related factors. Blocking SP1 activity by an inhibitor suppressed the starvation-plus-radiation treatment-mediated enhancement of U251 cell metastasis. Our study provides the first evidence that starvation caused by radiation might play a significant role in enhancing the ability of the glioma cell line U251 to metastasize via regulation of the transcription factor SP1.

  20. U251胶质瘤细胞系中脑肿瘤干细胞的分离、培养及鉴定%Isolation,Culture and Identification of Brain Tumor Stem Cellswithin U251 Glioma Cell Line In Vitro

    Institute of Scientific and Technical Information of China (English)

    陈成; 芦明; 李茗初; 方加胜

    2012-01-01

      Objective This study was to isolate, culture and identificate brain tumor stem cel s from U251 Gliome cel line in vitro and observe their growth pattern.To establish a fundation for further reseach of brain tumor stem cel . Methods U251cel s were seeded in serum-free DMEM-F12 medium supplemented with B27, EGF and bFGF in 6-wel plates, after U251 brain tumor spheres formed, they were dissociated and passaged in fresh medium periodical y. U251 brain tumor spheres and the single cel s of them were induced to differentiate in the medium with 10%FBS supplemented. U251cel s cultivate in serum-supplemented medium and serum-free medium alternately. At last we performed immunocytochemistry of U251 brain tumor spheres for CD133 and Nestin. Results Some U251 glioma cel have the capacity to self-renew, proliferate and generate free-floating neurosphere-like brain tumor spheres in serum-free medium in vitro. U251 glioma spheres and the single cel s of them can be induced to differentiate and adherent to the bottom of the culture plates. The growing patterns of U251 glioma cel line can be converted by changing the culture mediums. The brain tumor stem cel are CD133+Nestin+cel s. Conclusion The U251 glioma cel line contain brain tumor stem cel s which can be isolated, proliferated and differentiated in vitro. The research of brain tumor stem cel s may provide a new platform for brain tumor study.%  目的本研究旨在从U251胶质瘤细胞系中分离、培养和鉴定脑肿瘤干细胞,观察其的生长特征,为脑肿瘤干细胞的进一步研究打下基础。方法应用简化的无血清培养基和悬浮培养法培养U251细胞,并将获得的脑肿瘤干细胞接种于含血清培养基中观察其分化;交替用含血清培基和无血清培基培养U251细胞,观察其生长特性;用免疫荧光法检测脑肿瘤干细胞中CD133和Nestin的表达。结果 U251细胞接种至无血清培养基后,部分细胞能够存活并增殖为悬浮

  1. Apoptosis of Glioblastoma U251 Cells Induced by Carmustine Combined All-trans Retinoic Acid via Regulating Cyclin E and p27kip 1

    Institute of Scientific and Technical Information of China (English)

    QI Bin; WEIJun; HU Guo-zhang; YANG Hong-fa; BI Chun-hua; SUN Zhi-gang; TIAN Yu

    2011-01-01

    The effect and mechanism of carmustine(BCNU) combined with all-trans retinoic acid(ATRA) on the apoptosis of human glioblastoma U251 cells were investigated by means of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT) assay,flow cytometry,reverse transcription-polymerase chain reaction(RT-PCR) and Western blot analysis.The results show that BCNU or ATRA shows time- and dose-dependent inhibition effects on human glioblastoma U251 cells and the combination of BCNU with ATRA shows an synergistic inhibition effect on human glioblastoma U251 cells,and the combined BCNU and ATRA can significantly inhibit the proliferation of human glioblastoma U251 cells,and induce the apoptosis of them,making the cells arrest in the stage of G1 phase,the stage of S and G2 phases decline,the rate of the apoptosis of human glioblastoma U251 cells increase,the corresponding mRNA expression of cyclin E and cyclin-dependent kinase 2(CDK2) downregulated and the corresponding mRNA expression of p27kip 1 unregulated.In addition,the combined BCNU and ATRA reduced the protein expression of nuclear factor kappa B(NF-kB).Taken together,these results suggest that the treatment of human glioblastoma U251 cells with a combination application of ATRA and BCNU can exert synergistic effect,the course of this kind of combination chemotherapy may likely be associated with multiple molecular mechanisms for apoptosis,furthermore,the cyclin E and p27kip 1 should be considered as novel targets for controlling the growth of glioblastoma cells.

  2. Characterization of radioresistant variant from U251 human glioblastoma cell line and the role of antioxdant enzymes in its radioresistancy

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Hyung Chahn; Park, In Chul; Park, Myung Jin; Woo, Sang Hyeok; Rhee, Chang Hum; Hong, Seok-II [Korea Institute of Radiological and Medical Sciences, Seoul (Korea, Republic of)

    2004-07-01

    To investigate the radioresistant mechanism in glioblastoma multiforme(GBM), we isolated the radioresistant clone (RRC) from U251 human glioblastoma cell line by exposing to repeated fractions of 3 Gy {gamma}-radiation for six months. RRC had higher radioresistance than the parent cell line as measured by clonogenic survival assay. FACS analysis showed that RRC had a delayed G2 arrest after radiation. Antioxidant enzymes, such as SOD, catalase, glutathione peroxidase (GPX), glutathione reductase (GR), were activated up to 5 folds in RRC after radiation. Erk 1/2 activation was higher in RRC than in the parent cell. Therefore, radioresistancy in RRC might be due to the delayed cell cycle, the coordinated high activation of antioxidant enzyme rather than a single enzyme alone,and higher activation of Erk 1/2.

  3. Localization of phosphorylated TrkA in carrier vesicles involved in its nuclear translocation in U251 cell line

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    A number of transmembrane receptors are targeted to the nucleus and convincingly localized therein. However, what remains a conundrum is how these cell-surface receptors end up in the nucleus. In this study, we reported that the transmembrane receptor phosphorylated TrkA was located in a series of carrier vesicles, including ring-like vesicles near the plasma membrane, large core vesicles and small dense core vesicles around the nuclei, as well as in the nucleus in human glioma cell line U251 using immunocytochemistry and immunofluorescence staining. Meanwhile, we also showed that small dense core vesicles budded from large core vesicles, and interacted with the nuclear envelope. Accordingly, our results suggested that such a series of membrane compartments might be involved in the pathway of nuclear translocation of the transmembrane receptor TrkA.

  4. A vaccine against CCR5 protects a subset of macaques upon intravaginal challenge with simian immunodeficiency virus SIVmac251.

    Science.gov (United States)

    Van Rompay, Koen K A; Hunter, Zoe; Jayashankar, Kartika; Peabody, Julianne; Montefiori, David; LaBranche, Celia C; Keele, Brandon F; Jensen, Kara; Abel, Kristina; Chackerian, Bryce

    2014-02-01

    As an alternative to targeting human immunodeficiency virus (HIV), we have developed vaccines targeting CCR5, a self-protein critically involved in HIV replication and pathogenesis. By displaying peptides derived from CCR5 at high density on the surface of virus-like particles, we can efficiently induce high-titer IgG antibodies against this self-molecule. Here, we investigated whether prophylactic immunization of rhesus macaques with a particle-based vaccine targeting two regions of macaque CCR5 could prevent or suppress vaginal infection with highly virulent SIVmac251. Twelve macaques were vaccinated with a bacteriophage Qß-based vaccine targeting macaque CCR5 (Qß.CCR5). Six control animals were immunized with the Qß platform alone. All animals immunized with Qß.CCR5 developed high-titer anti-CCR5 antibody responses. Macaques were vaginally challenged with a high dose of SIVmac251. The mean peak viral RNA levels in the vaccinated groups were 30-fold lower than in the control group (10(6.8) versus 10(8.3) copies/ml plasma). Three of the 12 vaccinated macaques dramatically suppressed simian immunodeficiency virus (SIV) replication: peak viral loads were low (10(3) to 10(4) RNA copies/ml), and SIV RNA became undetectable from 6 weeks onward. No viral RNA or DNA could be detected in colon and lymph node biopsy specimens collected 13 months after challenge. In vivo depletion of CD8(+) cells failed to induce a viral rebound. However, once anti-CCR5 antibody responses had waned, the 3 animals became infected after intravaginal and/or intravenous rechallenge. In conclusion, vaccination against CCR5 was associated with dramatic suppression of virus replication in a subset (25%) of macaques. These data support further research of vaccination against CCR5 to combat HIV infection.

  5. 塞来昔布联合替莫唑胺对胶质瘤U251细胞作用研究%Effect of celecoxib combined with temozolomide on proliferation and apoptosis of glioma cell line U251 cells

    Institute of Scientific and Technical Information of China (English)

    何远志; 徐国政

    2012-01-01

    Objective To study the curative effects of celecoxib combined with antitumor drugs on gliomas and its mechanism. Methods The human glioma cell lines U251 cells were cultured and they treated with celecoxib or temozolomide or both the drugs. The cell morphologic change was observed under the optical microscope. The proliferation of U251 cells was determined by methyl thiaszolyl tetrazoliu assay. The migration ability of U251 cells was determined by wound healing test. The apoptosis of U251 cells were evaluated by flow cytometry with PI - Annexin V staining. The expression of Bcl-2 and Bax in U251 cells were detected by western blot. Results The morphologic changes occurred in U251 cells treated with celecoxib or temozolomide or both the drugs, but the morphologic change in U251 cells treated by both the drugs were most significant. The inhibitory effect of celecoxib combined with temozolomide on U251 cells proliferation was significantly stronger than those of celecoxib or temozolomide (P<0.01). The apoptosis rate in U251 cells treated by both the drugs was significantly higher than those in U251 cells treated by celecoxib or temozolomide alone (P<0.01). The celecoxib combined with temozolomide more significantly inhibited the migration of U251 cells compared to celecoxib or temozolomide alone. The level of Bax expression was significantly higher and the level of Bcl-2 expression was significantly lower in U251 cells treated by both the drugs than those in U251 cells treated by celecoxib or temozolomide alone. Conclusion The combinative application of celecoxib and temozolomide can promote the apoptosis and inhibit the proliferation and migration of U251 cells in a synergistic manner. These effects of celecoxib combined with temozolomide may be related with the upregnlation of Bax expression and down regulation of Bcl-2 expression.%目的 探讨环氧合酶抑制剂塞来昔布联合抗肿瘤药物替莫唑胺对神经胶质瘤细胞系U251细胞的体外抗瘤

  6. Mind Bomb-2 promotes U251 proliferation by regulating nuclear factor kappa B signaling pathways%Mind Bomb-2基因通过调控核因子κB信号通路促进 U251增殖的实验研究

    Institute of Scientific and Technical Information of China (English)

    张伯阳; 许重远

    2016-01-01

    Objective To study effects of Mind Bomb -2 ( MIB2 ) gene expression ’ s promotion on proliferation of U 251.Methods The expression of MIB2 protein in astrocyte and US251 was detected by Western blot.U251, U251 transduction with FLAG ( A 20 base coded marker protein ) plasmid and U251transfection with MIB2 -FLAG plasmid were divided into blank group , control group and test group correspondingly.The relative content of MIB 2 in three groups was detected by Western blotting .The MIB2 -FLAG plasmid was transfected to U251 cells.The real -time quantitative PCR was used to detect MIB 2 mRNA expression in U251.The expression of MIB2, I kappa B ( IkB) and nuclear factor kappa B ( NF-κB) was detected by Western blotting after transfection.MTT experiment was used to detect the proliferation of U251 cells after transfection.Results The relative content of MIB 2 in U251 cells increased by ( 17.04 ±2.91 ) compared with astrocytes.MIB2 mRNA in test group increased by ( 20.02 ±2.11 ) compared with control group.The expression of MIB 2 and NF-κB proteins in test group increased by ( 6.33 ±0.32 ) and ( 5.21 ±0.21 ) compared with control group, the expression of IkB in test group was attenuated by (0.43 ±0.04 ) compared with control group .Com-pared with both blank and control group , test group grew significantly ( P<0.001 ) .Conclusion MIB2 promoted U251 proliferation by regulating NF-κB signaling pathways.%目的:研究Mind Bomb-2(MIB2)的表达对脑胶质瘤细胞U251增殖的影响。方法用蛋白质印迹法检测正常星形胶质细胞和胶质瘤细胞U251和MIB2蛋白的表达情况。将U251细胞、转染FLAG (一种20个碱基编码的标记蛋白)质粒的U251细胞和转染MIB2-FLAG质粒的U251细胞对应分为空白组、对照组和实验组。以蛋白质印迹法检测上述3组中MIB2的相对含量;采用实时定量PCR技术检测MIB2 mRNA在3组U251细胞中的表达情况;以蛋白质印迹法检测转染后U251细胞中MIB2

  7. Common alleles at 6q25.1 and 1p11.2 are associated with breast cancer risk for BRCA1 and BRCA2 mutation carriers

    NARCIS (Netherlands)

    A.C. Antoniou (Antonis); C. Kartsonaki (Christiana); O. Sinilnikova (Olga); P. Soucy (Penny); L. McGuffog (Lesley); S. Healey (Sue); A. Lee (Andrew); P. Peterlongo (Paolo); S. Manoukian (Siranoush); B. Peissel (Bernard); D. Zaffaroni (D.); E. Cattaneo (Elisa); M. Barile (Monica); V. Pensotti (Valeria); B. Pasini (Barbara); R. Dolcetti (Riccardo); G. Giannini (Giuseppe); A.L. Putignano; L. Varesco (Liliana); P. Radice (Paolo); P.L. Mai (Phuong); M.H. Greene (Mark); I.L. Andrulis (Irene); G. Glendon (Gord); H. Ozcelik (Hilmi); M. Thomassen (Mads); A-M. Gerdes (Anne-Marie); T.A. Kruse (Torben); U.B. Jensen; D. Cruger (Dorthe); M.A. Caligo (Maria); Y. Laitman (Yael); R. Milgrom (Roni); B. Kaufman (Bella); S. Paluch-Shimon (Shani); E. Friedman (Eitan); N. Loman (Niklas); K. Harbst (Katja); A. Lindblom (Annika); B. Melin (Beatrice); K.L. Nathanson (Katherine); S.M. Domchek (Susan); R. Rebbeck (Timothy); A. Jakubowska (Anna); J. Lubinski (Jan); J. Gronwald (Jacek); T. Huzarski (Tomasz); T. Byrski (Tomasz); C. Cybulski (Cezary); B. Górski (Bohdan); A. Osorio (Ana); T.R. Cajal; F. Fostira (Florentia); R. Andres (Raquel); J. Benitez (Javier); U. Hamann (Ute); F.B.L. Hogervorst (Frans); M.A. Rookus (Matti); M.J. Hooning (Maartje); M.R. Nelen (Marcel); R.B. van der Luijt (Rob); T.A.M. van Os (Theo); C.J. van Asperen (Christi); P. Devilee (Peter); H. Meijers-Heijboer (Hanne); E.B.G. Garcia; S. Peock (Susan); M. Cook (Margaret); D. Frost; R. Platte (Radka); J. Leyland (Jean); D.G. Evans (Gareth); F. Lalloo (Fiona); R. Eeles (Rosalind); L. Izatt (Louise); R. Davidson (Rosemarie); D. Eccles (Diana); K.-R. Ong; F. Douglas (Fiona); J. Paterson (Joan); M.J. Kennedy (John); Z. Miedzybrodzka (Zosia); A.K. Godwin (Andrew); D. Stoppa-Lyonnet (Dominique); B. Buecher (Bruno); M. Belotti (Muriel); C. Tirapo (Carole); S. Mazoyer (Sylvie); L. Barjhoux (Laure); C. Lasset (Christine); D. Leroux (Dominique); L. Faivre (Laurence); M. Bronner (Myriam); F. Prieur (Fabienne); C. Nogues (Catherine); E. Rouleau (Etienne); P. Pujol (Pascal); I. Coupier (Isabelle); M. Frenay (Marc); J. Hopper (John); M.J. Daly (Mark); M-B. Terry (Mary-beth); E.M. John (Esther); S.S. Buys (Saundra); Y. Yassin (Yosuf); A. Miron (Alexander); D. Goldgar (David); C.F. Singer (Christian); M.-K. Tea; G. Pfeiler (Georg); C. Dressler (Catherina); T.V.O. Hansen (Thomas); L. Jønson (Lars); B. Ejlertsen (Bent); R.B. Barkardottir (Rosa); T. Kircchoff (Tomas); K. Offit (Kenneth); M. Piedmonte (Marion); G.C. Rodriguez (Gustavo); L. Small (Laurie); J.F. Boggess (John); S.V. Blank (Stephanie); J. Basil (Jack); M. Azodi (Masoud); A.E. Toland (Amanda); M. Montagna (Marco); S. Tognazzo (Silvia); S. Agata (Simona); E.N. Imyanitov (Evgeny); R. Janavicius (Ramunas); C. Lazaro (Conxi); I. Blanco (Ignacio); P.D.P. Pharoah (Paul); L. Sucheston (Lara); B.Y. Karlan (Beth); C.S. Walsh (Christine); E. Olah (Edith); A. Bozsik (Aniko); S.-H. Teo; J.L. Seldon (Joyce); M.S. Beattie (Mary); E.J. van Rensburg (Elizabeth); M.D. Sluiter (Michelle); O. Diez (Orland); R.K. Schmutzler (Rita); B. Wapenschmidt (Barbara); C. Engel (Christoph); A. Meindl (Alfons); I. Ruehl (Ina); R. Varon-Mateeva (Raymonda); K. Kast (Karin); H. Deissler (Helmut); D. Niederacher (Dieter); N. Arnold (Norbert); D. Gadzicki (Dorothea); I. Schönbuchner (Ines); T. Caldes (Trinidad); M. de La Hoya (Miguel); H. Nevanlinna (Heli); K. Aittomäki (Kristiina); M. Dumont (Martine); J. Chiquette (Jocelyne); M. Tischkowitz (Marc); G. Chenevix-Trench (Georgia); J. Beesley (Jonathan); A.B. Spurdle (Amanda); S.L. Neuhausen (Susan); Y.C. Ding (Yuan); Z. Fredericksen (Zachary); X. Wang (Xing); V.S. Pankratz (Shane); F.J. Couch (Fergus); J. Simard (Jacques); D.F. Easton (Douglas); G. Chenevix-Trench (Georgia); P. Karlsson (Per); M. Nordling (Margareta); A. Bergman (Annika); Z. Einbeigi (Zakaria); M. Stenmark-Askmalm (M.); S. Liedgren (Sigrun); Å. Borg (Åke); H. Olsson (Hans); U. Kristoffersson (Ulf); H. Jernström (H.); K. Henriksson (Karin); A. von Wachenfeldt (Anna); A. Liljegren (Annelie); G. Barbany-Bustinza (Gisela); J. Rantala (Johanna); H. Grönberg (Henrik); E.-L. Stattin; M. Emanuelsson (Monica); R.R. Brandell; N. Dahl (Niklas); S. Verhoef; M. Verheus (Martijn); L.J. van 't Veer (Laura); F.E. van Leeuwen; J.M. Collee (Margriet); A.M.W. van den Ouweland (Ans); A. Jager (Agnes); M.M.A. Tilanus-Linthorst (Madeleine); C.M. Seynaeve (Caroline); J.T. Wijnen (Juul); M.P. Vreeswijk (Maaike); R.A.E.M. Tollenaar (Rob); M.J. Ligtenberg (Marjolijn); N. Hoogerbrugge (Nicoline); M.G.E.M. Ausems (Margreet); C.M. Aalfs (Cora); J.J.P. Gille (Jan); Q. Waisfisz (Quinten); E.B. Gómez García (Encarna); C.E. van Roozendaal (Cees); M.J. Blok (Marinus); B. Caanen; J.C. Oosterwijk; A.H. van der Hout (Annemarie); M.J. Mourits; H.F. Vasen (Hans); H. Gregory (Helen); P.J. Morrison (Patrick); L. Jeffers (Lisa); T.J. Cole (Trevor); C. McKeown (Carole); J. Hoffman (Jonathan); A. Donaldson (Alan); S. Downing (Sarah); A. Taylor (Amy); A. Murray (Alexandra); M.T. Rogers (Mark); E. McCann (Emma); M.E. Porteous (Mary); S. Drummond (Sarah); C. Brewer (Carole); E. Kivuva (Emma); A. Searle (Anne); S. Goodman (Selina); K. Hill (Kathryn); V. Murday (Victoria); N. Bradshaw (Nicola); L. Snadden (Lesley); M. Longmuir (Mark); C. Watt (Catherine); S. Gibson (Sarah); E. Haque (Eshika); E. Tobias (Ed); A. Duncan (Alexis); C. Jacobs (Chris); C. Langman (Caroline); A. Whaite (Anna); H. Dorkins (Huw); J. Barwell (Julian); C. Chu (Chengbin); J. Miller (Julie); I.O. Ellis (Ian); C. Houghton (Catherine); L. Side (Lucy); A. Male (Alison); C. Berlin (Cheryl); J. Eason (Jacqueline); R. Collier (Rebecca); O. Claber (Oonagh); I. Jobson (Irene); L.J. Walker (Lisa); D. McLeod (Diane); D. Halliday (Dorothy); S. Durell (Sarah); B. Stayner (Barbara); S. Shanley (Susan); N. Rahman (Nazneen); R. Houlston (Richard); E. Bancroft (Elizabeth); L. D'Mello (Lucia); E. Page (Elizabeth); A. Ardern-Jones (Audrey); K. Kohut (Kelly); J. Wiggins (Jennifer); E. Castro (Elena); A. Mitra (Anita); L. Robertson (Lisa); O. Quarrell (Oliver); C. Bardsley (Cathryn); H. Ehrencrona (Hans); S.V. Hodgson (Shirley); D.E. Barton (David); S. Goff (Sheila); G. Brice (Glen); L. Winchester (Lizzie); C. Eddy (Charlotte); V. Tripathi (Vishakha); V. Attard (Virginia); A. Lucassen (Anneke); G. Crawford (Gillian); D. McBride (Donna); S. Smalley (Sarah); J.W. Adlard (Julian); B. Arver (Brita Wasteson)

    2011-01-01

    textabstractTwo single nucleotide polymorphisms (SNPs) at 6q25.1, near the ESR1 gene, have been implicated in the susceptibility to breast cancer for Asian (rs2046210) and European women (rs9397435). A genome-wide association study in Europeans identified two further breast cancer susceptibility var

  8. Long-lasting recovery of psychotic-like symptoms in isolation-reared rats after chronic but not acute treatment with the cannabinoid antagonist AM251.

    Science.gov (United States)

    Zamberletti, Erica; Viganò, Daniela; Guidali, Cinzia; Rubino, Tiziana; Parolaro, Daniela

    2012-03-01

    In this work we investigated the ability of AM251 to reverse schizophrenia-like symptoms produced by a neurodevelopmental animal model based on a social isolation procedure. First, we assessed the validity of our isolation-rearing protocol and, as expected, isolation-reared rats showed hyperlocomotion in a novel environment, cognitive impairment in the novel object recognition (NOR) test and a significant increase in the number of aggressive behaviours in the social interaction test compared to group-housed controls. This behavioural picture was associated with a reduction in CB₁ receptor/G protein coupling in specific brain areas as well as reduced c-Fos immunoreactivity in the prefrontal cortex and caudate putamen. In this model, chronic but not acute treatment with the CB₁ receptor antagonist AM251 counteracted isolation-induced cognitive impairment in the NOR test and aggressive behaviours in the social interaction test. This behavioural recovery was accompanied by the rescue of CB₁ receptor functionality and c-Fos levels in all brain regions altered in isolation-reared rats. Moreover, chronic AM251 also increased c-Fos immunoreactivity in the nucleus accumbens, as previously demonstrated for antipsychotic drugs. Interestingly, the behavioural recovery due to chronic AM251 administration persisted until 10 d after discontinuing the treatment, indicating a long-lasting effect of the cannabinoid antagonist on psychotic-like symptoms.

  9. Common alleles at 6q25.1 and 1p11.2 are associated with breast cancer risk for BRCA1 and BRCA2 mutation carriers

    DEFF Research Database (Denmark)

    Antoniou, Antonis C; Kartsonaki, Christiana; Sinilnikova, Olga M

    2011-01-01

    Two single nucleotide polymorphisms (SNPs) at 6q25.1, near the ESR1 gene, have been implicated in the susceptibility to breast cancer for Asian (rs2046210) and European women (rs9397435). A genome-wide association study in Europeans identified two further breast cancer susceptibility variants: rs...... to a better understanding of the biology of tumour development in these women....

  10. Common alleles at 6q25.1 and 1p11.2 are associated with breast cancer risk for BRCA1 and BRCA2 mutation carriers

    NARCIS (Netherlands)

    Antoniou, Antonis C.; Kartsonaki, Christiana; Sinilnikova, Olga M.; Soucy, Penny; McGuffog, Lesley; Healey, Sue; Lee, Andrew; Peterlongo, Paolo; Manoukian, Siranoush; Peissel, Bernard; Zaffaroni, Daniela; Cattaneo, Elisa; Barile, Monica; Pensotti, Valeria; Pasini, Barbara; Dolcetti, Riccardo; Giannini, Giuseppe; Putignano, Anna Laura; Varesco, Liliana; Radice, Paolo; Mai, Phuong L.; Greene, Mark H.; Andrulis, Irene L.; Glendon, Gord; Ozcelik, Hilmi; Thomassen, Mads; Gerdes, Anne-Marie; Kruse, Torben A.; Jensen, Uffe Birk; Crueger, Dorthe G.; Caligo, Maria A.; Laitman, Yael; Milgrom, Roni; Kaufman, Bella; Paluch-Shimon, Shani; Friedman, Eitan; Loman, Niklas; Harbst, Katja; Lindblom, Annika; Arver, Brita; Ehrencrona, Hans; Melin, Beatrice; Nathanson, Katherine L.; Domchek, Susan M.; Rebbeck, Timothy; Jakubowska, Ania; Lubinski, Jan; Gronwald, Jacek; Huzarski, Tomasz; Byrski, Tomasz; Cybulski, Cezary; Gorski, Bohdan; Osorio, Ana; Ramon y Cajal, Teresa; Fostira, Florentia; Andres, Raquel; Benitez, Javier; Hamann, Ute; Hogervorst, Frans B.; Rookus, Matti A.; Hooning, Maartje J.; Nelen, Marcel R.; van der Luijt, Rob B.; van Os, Theo A. M.; van Asperen, Christi J.; Devilee, Peter; Meijers-Heijboer, Hanne E. J.; Garcia, Encarna B. Gomez; Peock, Susan; Cook, Margaret; Frost, Debra; Platte, Radka; Leyland, Jean; Evans, D. Gareth; Lalloo, Fiona; Eeles, Ros; Izatt, Louise; Adlard, Julian; Davidson, Rosemarie; Eccles, Diana; Ong, Kai-ren; Cook, Jackie; Douglas, Fiona; Paterson, Joan; Kennedy, M. John; Miedzybrodzka, Zosia; Godwin, Andrew; Stoppa-Lyonnet, Dominique; Buecher, Bruno; Belotti, Muriel; Tirapo, Carole; Mazoyer, Sylvie; Barjhoux, Laure; Lasset, Christine; Leroux, Dominique; Faivre, Laurence; Bronner, Myriam; Prieur, Fabienne; Nogues, Catherine; Rouleau, Etienne; Pujol, Pascal; Coupier, Isabelle; Frenay, Marc; Hopper, John L.; Daly, Mary B.; Terry, Mary B.; John, Esther M.; Buys, Saundra S.; Yassin, Yosuf; Miron, Alexander; Goldgar, David; Singer, Christian F.; Tea, Muy-Kheng; Pfeiler, Georg; Dressler, Anne Catharina; Hansen, Thomas v. O.; Jonson, Lars; Ejlertsen, Bent; Barkardottir, Rosa Bjork; Kirchhoff, Tomas; Offit, Kenneth; Piedmonte, Marion; Rodriguez, Gustavo; Small, Laurie; Boggess, John; Blank, Stephanie; Basil, Jack; Azodi, Masoud; Toland, Amanda Ewart; Montagna, Marco; Tognazzo, Silvia; Agata, Simona; Imyanitov, Evgeny; Janavicius, Ramunas; Lazaro, Conxi; Blanco, Ignacio; Pharoah, Paul D. P.; Sucheston, Lara; Karlan, Beth Y.; Walsh, Christine S.; Olah, Edith; Bozsik, Aniko; Teo, Soo-Hwang; Seldon, Joyce L.; Beattie, Mary S.; van Rensburg, Elizabeth J.; Sluiter, Michelle D.; Diez, Orland; Schmutzler, Rita K.; Wappenschmidt, Barbara; Engel, Christoph; Meindl, Alfons; Ruehl, Ina; Varon-Mateeva, Raymonda; Kast, Karin; Deissler, Helmut; Niederacher, Dieter; Arnold, Norbert; Gadzicki, Dorothea; Schoenbuchner, Ines; Caldes, Trinidad; de la Hoya, Miguel; Nevanlinna, Heli; Aittomaki, Kristiina; Dumont, Martine; Chiquette, Jocelyne; Tischkowitz, Marc; Chen, Xiaoqing; Beesley, Jonathan; Spurdle, Amanda B.; Neuhausen, Susan L.; Ding, Yuan Chun; Fredericksen, Zachary; Wang, Xianshu; Pankratz, Vernon S.; Couch, Fergus; Simard, Jacques; Easton, Douglas F.; Chenevix-Trench, Georgia

    2011-01-01

    Two single nucleotide polymorphisms (SNPs) at 6q25.1, near the ESR1 gene, have been implicated in the susceptibility to breast cancer for Asian (rs2046210) and European women (rs9397435). A genome-wide association study in Europeans identified two further breast cancer susceptibility variants: rs112

  11. 75 FR 43818 - Amendment of VOR Federal Airways V-50, V-251, and V-313 in the Vicinity of Decatur, IL

    Science.gov (United States)

    2010-07-27

    ... as part of the V-50, V-251, and V-313 route structure, is being renamed the Adders VORTAC. DATES... Decatur VORTAC will be renamed the Adders VORTAC and assigned a new facility identifier (AXC). All VOR... Adders, IL, name change. The name change of the VORTAC will coincide with the effective date of...

  12. Modulation of voltage-gated Na+ and K+ channels by pumiliotoxin 251D: a "joint venture" alkaloid from arthropods and amphibians.

    Science.gov (United States)

    Vandendriessche, Thomas; Abdel-Mottaleb, Yousra; Maertens, Chantal; Cuypers, Eva; Sudau, Alexander; Nubbemeyer, Udo; Mebs, Dietrich; Tytgat, Jan

    2008-03-01

    Certain amphibians provide themselves with a chemical defense by accumulating lipophilic alkaloids into skin glands from dietary arthropods. Examples of such alkaloids are pumiliotoxins (PTXs). In general, PTXs are known as positive modulators of voltage-gated sodium channels (VGSCs). Unlike other PTXs, PTX 251D does not share this characteristic. However, mice and insect studies showed that PTX 251D is highly toxic and to date the basis of its toxicity remains unknown. In this work, we searched for the possible target of PTX 251D. The toxin was therefore made synthetically and tested on four VGSCs (mammalian rNa(v)1.2/beta(1), rNa(v)1.4/beta(1), hNa(v)1.5/beta(1) and insect Para/tipE) and five voltage-gated potassium channels (VGPCs) (mammalian rK(v)1.1-1.2, hK(v)1.3, hK(v)11.1 (hERG) and insect Shaker IR) expressed heterologously in Xenopus laevis oocytes, using the two-electrode voltage clamp technique. PTX 251D not only inhibited the Na(+) influx through the mammalian VGSCs but also affected the steady-state activation and inactivation. Interestingly, in the insect ortholog, the inactivation process was dramatically affected. Additionally, PTX 251D inhibited the K(+) efflux through all five tested VGPCs and slowed down the deactivation kinetics of the mammalian VGPCs. hK(v)1.3 was the most sensitive channel, with an IC(50) value 10.8+/-0.5 microM. To the best of our knowledge this is the first report of a PTX affecting VGPCs.

  13. The polymorphism interleukin-8 -251A/T is associated with a significantly increased risk of cancers from a meta-analysis.

    Science.gov (United States)

    Wang, Ziliang; Liu, Yang; Yang, Lina; Yin, Sheng; Zang, Rongyu; Yang, Gong

    2014-07-01

    Emerging evidences show that interleukin-8 (IL-8) has important regulatory functions in tumorigenesis. IL-8 -251A/T is a single nucleotide polymorphism in the promoter region of the IL-8 gene and affects IL-8 production. Analysis of previous studies on the association of -251A/T polymorphism with different cancer types remained to be illustrated. To further assess the effect of -251A/T polymorphism on cancer risks, we performed this meta-analysis, up to November 2013, of 12,917 cases with different cancer types and 17,689 controls from 47 published case-control designed studies. Statistical analyses were performed using STATA 11.0 software. Crude odds ratios (ORs) with 95 % confidence intervals (CIs) were used to assess the strength of associations. ORs with 95 % CIs for IL-8 -251A/T polymorphism and cancer were estimated using fixed- and random-effects models when appropriate. Significantly increased risks were found in overall under the models of A allele vs. T allele, AA vs. TT, and AA vs. AT/TT. Significantly elevated risks were observed in breast cancer under the models of A allele vs. T allele, AT vs. TT, AA/AT vs. TT, and AA vs. AT/TT, and in nasopharyngeal carcinoma under the models of AT vs. TT, AA/AT vs. TT, and AA vs. AT/TT. We found that significantly elevated risks were observed in the Asian population and hospital-based studies in all comparison models. Thus, this meta-analysis indicates that IL-8 -251A/T polymorphism is associated with a significantly increased risk of cancers and may provide evidence-based medical certificate to study the cancer susceptibility.

  14. EGCG诱导人胶质瘤细胞U251凋亡及对survvin表达的影响%Induction of Apoptosis in Glioblastoma Cell line U251 of Epigallocatechin-3-gallate and its Influence on the Expressions of Surviv in Protein

    Institute of Scientific and Technical Information of China (English)

    赵志远; 谢瑞; 王宇

    2012-01-01

    目的:观察表没食子儿茶素没食子酸酯(EGCG)诱导人胶质瘤细胞(U251)凋亡的生物学效应及对survivin蛋白表达的影响.方法:采用MTT法检测不同浓度EGCG对U251细胞增殖抑制作用;吖啶橙/溴化乙啶(AO/EB)荧光双染法观察各组细胞凋亡形态,计算凋亡率;Wester Blot法检测不同剂量EGCG处理48 h对survivin蛋白表达的影响.结果:EGCG显著抑制U251细胞生长,呈浓度依赖性;EGCG可以诱导U251细胞凋亡,细胞凋亡率随着药物浓度的增加而逐渐增加.Wester Blot结果显示EGCG抑制Survivin蛋白的表达.结论:EGCG具有诱导U251细胞凋亡的作用,其机制可能与抑制Survivin蛋白的表达相关.

  15. The influence of the combined treatment with Vadimezan (ASA404 and taxol on the growth of U251 glioblastoma xenografts

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    Milanović Dušan

    2012-06-01

    Full Text Available Abstract Background One of the most important biological characteristics of Glioblastoma multiforme (GBM is high vascular density. Vadimezan (ASA404, DMXAA belongs to the class of small molecule vascular disrupting agents (VDA that cause disruption of established tumor vessels and subsequent tumor hemorrhagic necrosis. Its selective antivascular effect is mediated by intratumoral induction of several cytokines including tumor necrosis factor-α (TNF-α, granulocyte-colony-stimulating factor (G-CSF, interleukin 6 (IL-6 and macrophage inflammatory protein 1α (MIP-1α. Preclinical studies have demonstrated that ASA404 acts synergistically with taxanes. In this study, we investigated if treatment of mice bearing U251 human glioblastoma xenografts with ASA404 and taxol may be synergistic. Therapy response was evaluated by measuring changes in tumor size and metabolic activity using 18F-FDG PET (Fluorodeoxyglucose - positron emision tomography imaging. Methods U251 cells were inoculated s.c. in the right hind limb of NMRI-Foxn1nu athymic female nude mice. Animals were randomly assigned into 4 groups (7–9 animals/group for treatment: control, taxol, ASA404, and ASA404 plus taxol. The animals received either a single dose of taxol (10 mg/kg, ASA404 (27.5 mg/kg, or taxol (10 mg/kg plus ASA404 (27.5 mg/kg administered i.p.; ASA404 was administred 24 h after the treatment with taxol. 4 and 24 h after treatment with ASA404 (28 and 48 h hours after treatment with taxol 18 F-FDG PET scans were performed. Results The treatment with taxol did not affect the tumor growth in comparison to untreated controls. The treatment of animals with single dose ASA404 alone or in combination with taxol caused a significant delay in tumor growth. The combined treatment did not decrease the growth of the xenografts significantly more than ASA404 alone, but early changes in tumor 18 F-FDG uptake preceded subsequent growth inhibition. The tumor weights

  16. Spectroscopy of odd Z trans-fermium nuclei: the nuclear structure of Md{sup 251}; Spectroscopie des transfermiums impairs en proton: la structure du noyau de {sup 251}Md

    Energy Technology Data Exchange (ETDEWEB)

    Chatillon, A

    2005-10-01

    The objective of this thesis was to determine the structure of trans-fermium nuclei (Z 100) with odd proton number, which remained largely unexplored. These nuclei were produced in fusion-evaporation reactions with small cross sections below 1 {mu}b. The experimental methods of Recoil-Tagging and Recoil-Decay-Tagging were used for their identification. In order to identify the active orbitals in this mass region, {sup 255}Lr, {sup 251}M1d and {sup 247}Es nuclei have been studied by decay spectroscopy at the University of Jyvaskyla and at GANIL with the LISE spectrometer and the {alpha}-electron detector BEST coupled to four CLover detectors from the EXOGAM array. New states have been observed in each of the isotopes, and their configuration has been proposed. The collective properties were also studied in two experiments using prompt {gamma} and electron spectroscopy, combining the JUROGAM and SACRED arrays, respectively, with the recoil separator RITU and the GREAT spectrometer at its focal plane. A rotational band has been observed for the first time in a proton-odd trans-fermium nucleus. The interpretation of this collective structure is based on the theoretical HFB calculations. (author)

  17. Autosomal recessive spastic paraplegia (SPG45) with mental retardation maps to 10q24.3-q25.1.

    Science.gov (United States)

    Dursun, Umut; Koroglu, Cigdem; Kocasoy Orhan, Elif; Ugur, Sibel Aylin; Tolun, Aslihan

    2009-10-01

    Hereditary spastic paraplegias (HSPs) are characterized by progressive spasticity in the lower limbs. They are clinically heterogeneous, and pure forms as well as complicated forms with other accompanying clinical findings are known. HSPs are also genetically heterogeneous. We performed clinical and genetic studies in a consanguineous family with five affected members. A genome scan using 405 microsatellite markers for eight members of the family identified candidate gene loci, and subsequent fine mapping in 16 members identified the gene locus responsible for the HSP. The clinical manifestations were very early onset spastic paraplegia (SPG) accompanied by mental retardation and ocular signs. The gene locus was identified as the interval 102.05-106.64 Mbp on chromosome 10. Gene MRPL43 was analyzed in the patients. No mutation but high levels of mRNA were detected. We have mapped a novel autosomal recessive complicated form of HSP (SPG45) to a 4.6-Mbp region at 10q24.3-q25.1 with multipoint logarithm of odds scores >4.5.

  18. Interleukin-8-251T > a, interleukin-1α-889C > t and apolipoprotein e polymorphisms in Alzheimer's disease

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    Alex Augusto Vendramini

    2011-01-01

    Full Text Available An inflammatory process has been involved in numerous neurodegenerative disorders such as Parkinson's disease, stroke and Alzheimer's disease (AD. In AD, the inflammatory response is mainly located in the vicinity of amyloid plaques. Cytokines, such as interleukin-8 (IL-8 and interleukin-1α (IL-1α, have been clearly involved in this inflammatory process. Polymorphisms of several interleukin genes have been correlated to the risk of developing AD. The present study investigated the association of AD with polymorphisms IL-8 -251T > A (rs4073 and IL-1α-889C > T (rs1800587 and the interactive effect of both, adjusted by the Apolipoprotein E genotype. 199 blood samples from patients with AD, 146 healthy elderly controls and 95 healthy young controls were obtained. DNA samples were isolated from blood cells, and the PCR-RFLP method was used for genotyping. The genotype distributions of polymorphisms IL-8, IL-1α and APOE were as expected under Hardy-Weinberg equilibrium. The allele frequencies did not differ significantly among the three groups tested. As expected, the APOE4 allele was strongly associated with AD (p A and IL-1α-889C > T were not found to be risk factors for AD.

  19. Low dose rectal inoculation of rhesus macaques by SIV smE660 or SIVmac251 recapitulates

    Energy Technology Data Exchange (ETDEWEB)

    Hraber, Peter [Los Alamos National Laboratory; Giorgi, Elena E [Los Alamos National Laboratory; Keele, Brandon [UNIV OF ALABAMA; Li, Hui [UNIV OF ALABAMA; Learn, Gerald [UNIV OF ALABAMA

    2008-01-01

    We recently developed a novel strategy to identify transmitted HIV-1 genomes in acutely infected humans using single-genome amplification and a model of random virus evolution. Here, we used this approach to determine the molecular features of simian immunodeficiency virus (SIV) transmission in 18 experimentally infected Indian rhesus macaques. Animals were inoculated intrarectally (i.r.) or intravenously (i.v.) with stocks of SIVmac251 or SIVsmE660 that exhibited sequence diversity typical of early-chronic HIV-1 infection. 987 full-length SIV env sequences (median of 48 per animal) were determined from plasma virion RNA 1--5 wk after infection. i.r. inoculation was followed by productive infection by one or a few viruses (median 1; range 1--5) that diversified randomly with near starlike phylogeny and a Poisson distribution of mutations. Consensus viral sequences from ramp-up and peak viremia were identical to viruses found in the inocula or differed from them by only one or a few nucleotides, providing direct evidence that early plasma viral sequences coalesce to transmitted/founder viruses. i.v. infection was >2,000-fold more efficient than i.r. infection, and viruses transmitted by either route represented the full genetic spectra of the inocula. These findings identify key similarities in mucosal transmission and early diversification between SIV and HIV-1, and thus validate the SIV-macaque mucosal infection model for HIV-1 vaccine and microbicide research.

  20. Research on biological functions of U251 astroglioma cells infected with human herpesvirus type 6(HHV-6)%人类疱疹病毒6型感染U251细胞及其生物学功能的研究

    Institute of Scientific and Technical Information of China (English)

    郭一迪; 姚堃; 周锋; 李凌云; 茌静; 刘根焰; 陈云

    2010-01-01

    目的:研究人类疱疹病毒6型(HHV-6)感染人星形胶质瘤细胞U251,及感染后对其生物学功能的影响.方法:HHV-6感染U251细胞建立HHV-6体外感染模型,倒置显微镜观察细胞病变.PCR法检测HHV-6 u22基因.间接免疫荧光(IFA)检测HHV-6即刻早期蛋白(IEI)和晚期蛋白(gB)的表达.MTT法检测U251感染HHV-6后细胞增殖的改变.流式细胞术(FCM)检测U251细胞周期改变.结果:在感染3天后U251出现典型细胞病变,细胞肿胀增大数倍,透亮,呈多形性.PCR检测到HHV-6u22基因.IFA检测到HHV-6 IE1蛋白和异B蛋白的表达.MTT法显示HHV-6能增强U251细胞的增殖能力.FCM检测表明,HHV-6感染后U251细胞周期发生改变,感染组和对照组相比,前者G1期的细胞减少,S期和G2期细胞增多.结论:HHV-6能感染U251细胞引起细胞病变.促进细胞的增殖.使细胞周期发生改变.本研究结果提示人类疱疹病毒6型可能参与人神经胶质瘤的发生、发展,对其机制尚不清楚.

  1. Influence of hyperbaric oxygen combining Temozolomide on human glioblastoma cell line U251%高压氧联合替莫唑胺对胶质瘤U251细胞的影响

    Institute of Scientific and Technical Information of China (English)

    曹侃; 袁志诚; 陆新宇; 李慧勇

    2012-01-01

    Objective To discuss the influence and mechanism on hyperbarie oxygen ( HBO) combining Temozolomide with human glioblastoma cell line U251. Method The cells were divided into four groups,A group is HBO combining Temozolomide,B group is HBO,C group is Temozolomide and D group is control group. By simulation of hypoxia microenviroment in vitro, the rate of growth inhibiting is investigated by MTT method. The rate of cell death is observed by PI staining. The rate of apoptosis is analyzed by the flow cytometry. Elasia method is used to detect the expression of hypoxia-inducible factorl-α (HIFl-α) and Multidrug resistance-associated protein-1 ( MRP-1). Results The group of HBO combining Temozolomide is obviously higher than others in rate of growth inhibiting, rate of cell death, rate of apoptosis, the results have statistics differences(P 0. 05) , but to others it has statistics value (P < 0. 05). Conclusion HBO can enhance the chemotherapy effect of temozolomide, it can correct the hypoxic microenvironment and reduce the expression of HIFl-α and MRP-1 ,these may be the key to effect.%目的 研究高压氧( Hyperbaric Oxygen,HBO)联合替莫唑胺(Temozolomide)对人神经胶质瘤U251细胞株的的影响及其机制.方法 实验分为4组:A组高压氧联合替莫唑胺组、B组高压氧组、C组替莫唑胺组、D组对照组.通过体外模拟缺氧微环境,用四甲基偶氮唑蓝法(MTT法)、碘化丙啶(PI)染色法、流式细胞仪分别检测细胞生长抑制率、细胞死亡率、细胞凋亡率.Elisa法检测缺氧诱导因子1-α(HIF1-α)和多药耐药相关蛋白-1(MRP-1)的表达情况.结果 1高压氧联合替莫唑胺组在细胞生长抑制率、细胞死亡率和细胞凋亡率上明显高于其他组,数值均有统计学意义(P<0.05).2高压氧联合替莫唑胺组与高压氧组在HIF1-α和MRP-1上无统计学意义(P>0.05),与其他组有统计学意义(P<0.05).结论 高压氧够增强替莫唑胺化疗效果,高压氧纠

  2. A clinical epidemiological study of 251 cases of amyotrophic lateral sclerosis in the south of Brazil Estudo clínico epidemiológico de 251 casos de esclerose lateral amiotrófica no sul do Brasil

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    Lineu Cesar Werneck

    2007-06-01

    Full Text Available OBJECTIVE: To study the clinical forms of amyotrophic lateral sclerosis (ALS and the possible presence of risk factors in order to verify if there is any difference between cases in Paraná, Brazil. METHOD: We studied 251 cases, all of which fulfilled the diagnosis criteria proposed in El Escorial (WFN. Between 1977 and 2004, 157 male and 94 female patients were examined. RESULTS: 220 cases were classified as ALS-Spinal Onset (ALS-SO, 24 as ALS-Bulbar Onset (ALS-BO and 7 as Familial ALS. The mean age at time of evaluation was 54.4±12.3 years, and symptoms had started 17.9±15.7months previously. In the group studied, statistical relationships were found between heavy occupations and males; previous surgeries and females; ALS-BO and dysphagia and dysarthria in females; and ALS-SO and males, cramps, weakness, muscle atrophy, hypertonia, increased deep tendon reflex and abnormal gait. CONCLUSION: The average age at time of evaluation was lower than that registered in the literature but similar to the Brazilian series. Domestic work and heavy occupations appear to be related to precocious perception of the symptoms by interference with daily functions. The socioeconomically higher classes seek medical care early. There was no relationship with exposure to toxic agents or trauma.OBJETIVO: Estudar as formas clínicas de esclerose lateral amiotrófica (ELA e possíveis fatores de risco, a fim de verificar se existem diferenças entre os casos do Paraná, Brasil. MÉTODO: Estudamos 251 casos entre 1977 e 2004, que preencheram os critérios propostos em El Escorial (WFN, sendo 157 do sexo masculino e 94 do feminino. RESULTADOS: Foram classificados como ELA de início espinhal (ELA-IE 220 casos, ELA de início bulbar (ELA-IB 24 casos e 7 casos como ELA familiar. A idade média na avaliação foi 54,4±12,3 anos cujos sintomas iniciaram 17,9 ±15,7 meses antes. Foram encontradas relações estatísticas entre ocupação que demandam esforços físicos com

  3. Identification of a 251 gene expression signature that can accurately detect M. tuberculosis in patients with and without HIV co-infection.

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    Noor Dawany

    Full Text Available BACKGROUND: Co-infection with tuberculosis (TB is the leading cause of death in HIV-infected individuals. However, diagnosis of TB, especially in the presence of an HIV co-infection, can be limiting due to the high inaccuracy associated with the use of conventional diagnostic methods. Here we report a gene signature that can identify a tuberculosis infection in patients co-infected with HIV as well as in the absence of HIV. METHODS: We analyzed global gene expression data from peripheral blood mononuclear cell (PBMC samples of patients that were either mono-infected with HIV or co-infected with HIV/TB and used support vector machines to identify a gene signature that can distinguish between the two classes. We then validated our results using publically available gene expression data from patients mono-infected with TB. RESULTS: Our analysis successfully identified a 251-gene signature that accurately distinguishes patients co-infected with HIV/TB from those infected with HIV only, with an overall accuracy of 81.4% (sensitivity = 76.2%, specificity = 86.4%. Furthermore, we show that our 251-gene signature can also accurately distinguish patients with active TB in the absence of an HIV infection from both patients with a latent TB infection and healthy controls (88.9-94.7% accuracy; 69.2-90% sensitivity and 90.3-100% specificity. We also demonstrate that the expression levels of the 251-gene signature diminish as a correlate of the length of TB treatment. CONCLUSIONS: A 251-gene signature is described to (a detect TB in the presence or absence of an HIV co-infection, and (b assess response to treatment following anti-TB therapy.

  4. The effects of TSA combined with HSV-1 on proliferation and apoptosis of U251 cells%曲古抑菌素A联合单纯疱疹病毒Ⅰ型对U251细胞增殖、凋亡的影响

    Institute of Scientific and Technical Information of China (English)

    许强华; 陈新军

    2012-01-01

    Objective To observe the effects of trichostain A (TSA) combined with herpes simplex virus-1 ( HSV-1 ) on proliferation and apoptosis in U251 cells in vitro.Methods U251 cells in vitro were treated separately with 0.5 × 10-3 μmol/L TSA (TSA group),10 MOI HSV-1 (HSV-1 group),and 0.5 × 10-3 μmol/L TSA combined with 10 MOI HSV-1 (TSA + HSV-1 group) for 72 h.The methyl thiazol tetrazolium (MTT) assay was used to observe the proliferation of U251 cells.The cellular morphological changes were observed under a microscope.The apoptosis rate was analyzed by using flow cytometry.Results In TSA group,HSV-1 group,TSA + HSV-1 group,the proliferation inhibition rate of the U251 cells was (48.0t1.8)%,(18.0 ±3.1)%,and (58.0t5.8)%; Apoptosis rate was (47.4±3.5)%,(29.6 ±3.0)%,and (59.6 ±4.0)% respectively.The inhibitory effects of proliferation or apoptosis in TSA + HSV-1 group was stronger than TSA group or HSV-1 group ( P < 0.01 ).Conclusion TSA combined with HSV-1 has a synergistic killing effect on cultured U251 cells.%目的 以体外培养的U251细胞为模型,观察曲古抑菌素A(TSA)联合单纯疱疹病毒Ⅰ型( HSV-1)对肿瘤细胞增殖、凋亡的影响.方法 以0.5 ×10-3μmol/L TSA和10 MOI HSV-1及0.5 × 101 μmol/L TSA与10 MOIHSV-1组合作用于U251人胶质瘤细胞,72 h后噻唑蓝(MTT)比色法检测各组细胞增殖情况,显微镜下观察各组细胞形态,流式细胞仪检测各组细胞的凋亡情况.结果 单一TSA组、单一HSV-1组、TSA与HSV-1联合组U251细胞增殖抑制率分别为(48.0±1.8)%、(18.0±3.1)%、(58.0±5.8)%;凋亡率分别为(47.4±3.5)%、(29.6±3.0)%、(59.6±4.0)%.TSA与HSV-1联合组U251细胞增殖抑制率及凋亡率明显高于单一使用TSA或HSV-1组(P<0.01).结论 TSA联合HSV-1对体外培养的U251细胞具有协同或叠加杀伤作用.

  5. -251 T/A polymorphism of the interleukin-8 gene and cancer risk: a HuGE review and meta-analysis based on 42 case-control studies.

    Science.gov (United States)

    Wang, Na; Zhou, Rongmiao; Wang, Chunmei; Guo, Xiaoqing; Chen, Zhifeng; Yang, Shan; Li, Yan

    2012-03-01

    The -251T/A (rs4073), a single nucleotide polymorphism, has been identified in the promoter region of the interleukin-8 (IL-8) gene. It's presence could influence the production of IL-8 protein by regulating the transcriptional activity of the gene. A large number of studies have been performed to evaluate the role of -251T/A polymorphism on various cancers, with inconsistent results being reported. In this paper, we summarized 13,189 cases and 16,828 controls from 42 case-control studies and attempted to assess the susceptibility of -251T/A polymorphism to cancers by a comprehensive meta-analysis. Pooled odds ratios and 95% confidence intervals were calculated by using the random-effects model. Publication bias, subgroup, and sensitivity analysis were also performed. Results showed that the carriers of the -251A allele had about a 12-21% increased risk for the reviewed cancer, in total. The carriers of -251A had an elevated risk to breast cancer, gastric cancer and nasopharyngeal cancer and a reduced risk to prostate cancer, but no evidence was found to indicate that the -251A allele predisposed its carriers to colorectal and lung cancers. When stratified separately by 'racial descent' and 'study design', it was found that the carriers of the -251A allele among the African group, Asian group and hospital-based case-control study group were at a higher risk for cancer, but not in European group and population-based case-control study. These results show that -251A allele is susceptible in the development of low-penetrance cancers.

  6. Characterization of the SIVmac251 in a Chinese-origin rhesus macaque showing severe neurological symptoms and sequence variation of Gp120%表现神经症状的SIVmac251感染猴大脑基底节病毒gp120序列变异分析

    Institute of Scientific and Technical Information of China (English)

    刘克剑; 丛喆; 金光; 王卫; 陈霆; 蒋虹; 魏强

    2012-01-01

    Objective To investigate the possible neurotropism, neuroinvasion and their mechanism in a Chinese-origin rhesus macaque infected with SIVmac251 showing neurological symptoms. Methods Eight healthy laboratory rhesus macaques were infected with SIVmac251-155p6N by intravenous injection. Among them 3 monkeys showed neurological symptoms to a different degree, and one of these 3 animals (R21755) developed severe neurological symptoms. This monkey was sacrificed and the virus was isolated from the basal ganglia of the brain, the plasma viral load was quantified by real-time RT-PCR, and T cell subsets were determined by flow cytometry. The brain lesions were examined by histopathology using HE staining. The RNAs were extracted from SIVmac251-155p6N, plasma and basal ganglia, the gpl20 genes were amplified with single genome amplification, and the changes of JV-glycosylation site in gp20 regions were analyzed. Results The monkey K21755 presented AIDS encephalopathy-like symptoms after half a year post the viral infection. Pathological examination showed infiltration of macrophages and multinucleated giant cells in perivascular space, and degeneration and necrosis of neurons were observed in the brain tissue. The gp120 sequence analysis showed that the virus isolated from the brain was partly different from that of the other sequences of SIVmac251-155p6N and plasma virus of the monkey R21755. The variations were mainly in the V1/V4 regions and the loss of a N-glycosylation site in era Cl. Conclusions It seems that the neuroinvasion and neurovinilence of SIVmac2Sl are significantly enhanced during the long-term adaption in vivo. The finding of this study provide a firm molecular basis for establishing a strain of SIVmac251 with neurotropism and neurovinilence. This would be very meaningful for investigating the role of SIV in neurologic disease and studies of AIDS neuropathy.%目的 研究猴免疫缺陷病毒SIVmac251在中国恒河猴感染传代过程中产生的可能

  7. Study on mechanism of temozolomide-resistant human glioma cell line U251/TR changed by temozolomide in vitro%替莫唑胺改变人胶质瘤细胞系U251耐药机制的体外研究

    Institute of Scientific and Technical Information of China (English)

    潘强; 杨学军; 高松; 纪延伟; 张文高; 李瑜; 王维; 董雪涛; 王华民

    2009-01-01

    目的 建立替莫唑胺耐药人胶质瘤细胞系,探讨其耐药性变化规律及耐药机制,以期为临床优化药物化疗方案提供理论依据.方法 采用分步诱导法使人胶质瘤细胞系U251对替莫唑胺耐药,磺酰罗丹明B比色法检测细胞耐药指数和存活率;Western blotting法检测O6-甲基鸟嘌呤-DNA甲基转移酶(MGMT)表达水平,以及倍增替莫唑胺终浓度后MGMT表达水平.结果 成功建立替莫唑胺耐药U251/TR细胞,在替莫唑胺初始诱导剂量0.25μg/ml、终浓度16.00μg/ml培养液中,U251/TR细胞半数抑制浓度为(220.87±2.34)μmol/L,约为U251细胞[(33.12±1.52)μmol/L]的7倍(t=-116.542,P=0.000),其耐药指数约为7;U251/TR细胞MGMT表达水平为(1.47±0.30),较U251细胞(0.19±0.03)明显升高(t=-20.230,P=0.000).与溶媒对照组比较,经倍增替莫唑胺终浓度诱导的U251/TR细胞仍呈现增殖抑制现象,以体外培养第3天时最为明显(P=0.000);MGMT表达水平相应降低(均P=0.000),呈现自身克服耐药现象.结论 采用分步诱导法可于体外成功建立替莫唑胺耐药人胶质瘤细胞系.MGMT表达水平升高是导致U251/TR细胞对替莫唑胺耐药的主要机制,替莫唑胺可以通过自身消耗MGMT而改变U251/TR细胞的耐药特性,发挥抗耐药作用.

  8. Study on mechanism of thalidomide combined with temozolomide to suppress proliferation of U251 glioma cell in vitro%沙利度胺联合替莫唑胺杀伤U251胶质瘤细胞机制的体外研究

    Institute of Scientific and Technical Information of China (English)

    高松; 潘强; 曾峥; 孙健; 杨学军

    2010-01-01

    目的 对沙利度胺联合替莫唑胺杀伤U251胶质瘤细胞的机制进行体外研究,为制订沙利度胺与替莫唑胺联合化疗方案提供理论依据.方法 经体外培养的人胶质瘤细胞系U251分别接受替莫唑胺(100 μmol/L)、沙利度胺(100 μg/L)、替莫唑胺与沙利度胺联合治疗,噻唑监(MTT)法检测不同抗肿瘤药物处理组肿瘤细胞增殖活性;流式细胞术分析细胞增殖周期;检测经吖啶橙标记的酸性囊性细胞器数目;原位末端标记(TUNEL)法观察肿瘤细胞凋亡情况;Western blotting法榆测肿瘤自噬及凋亡相关蛋白表达变化.结果 与替莫唑胺和沙利度胺单药治疗相比,替莫唑胺与沙利度胺联合治疗对U251细胞生长的抑制更为明显(均P=0.000).且可诱导肿瘤细胞周期阻滞于G0~G1期,以及发生凋亡和自噬.两药联合治疗后,U251细胞微管相关蛋白1轻链3和Caspase-3表达水平高于替莫唑胺组和沙利度胺组(均P=0.000).结论 沙利度胺联合替莫唑胺治疗U251细胞可以上调自噬及凋亡相关基因表达水平.同时诱导凋亡及自噬性死亡,从而达到对U251胶质瘤细胞的杀伤作用.

  9. Systemic or intra-amygdala infusion of an endocannabinoid CB1 receptor antagonist AM251 blocked propofol-induced anterograde amnesia.

    Science.gov (United States)

    Ren, Y; Wang, J; Xu, P B; Xu, Y J; Miao, C H

    2015-01-01

    Propofol is well-known for its anterograde amnesic actions. However, a recent experiment showed that propofol can also produce retrograde memory enhancement effects via an interaction with the endocannabinoid CB1 system. Therefore, the authors hypothesized that the regulating effect of propofol on the endocannabinoid CB1 system might also decrease the anterograde amnesic effect of propofol under some conditions, which might be a risk factor for intraoperative awareness. Since, the basolateral amygdala (BLA) has been confirmed to mediate propofol-induced anterograde amnesia and the BLA contains a high concentration of CB1 receptors, the authors investigated whether and how the endocannabinoid system, particularly the CB1 receptor within BLA, influences propofol-induced anterograde amnesia. Male Sprague-Dawley rats trained with inhibitory avoidance (IA) were systematically pre-trained using a memory-impairing dose of propofol (25 mg/kg). Before propofol administration, rats received an intraperitoneal injection of a CB1 receptor antagonist AM251 (1 mg/kg or 2 mg/kg) or a bilateral intra-BLA injection of AM251 (0.6 ng or 6 ng per 0.5 μl). Twenty-four hours after IA training, the IA retention latency was tested. It was found that systemic or intra-BLA injection of a non-regulating dose of AM251 (2 mg/kg or 6 ng per 0.5 μl, respectively) blocked the memory-impairing effect of propofol. These results indicate that the anterograde amnesic effect of propofol is mediated, in part, by activation of the CB1 cannabinoid receptors in the BLA.

  10. 乳腺丝氨酸蛋白酶抑制剂基因在胶质瘤细胞株U87、U251中表达沉默的表观遗传学机制%Epigenetics of silenced expression of maspin in glioma cell lines U87 and U251

    Institute of Scientific and Technical Information of China (English)

    刘泓渊; 屈鸣麒; 余聚; 兰青; 步星耀

    2014-01-01

    Objective To study the relationship between the silenced expression of mammary serine protease inhibitor (maspin) and epigenetics in human glioma cell lines U87 and U251.Methods Reverse transcription polymerase chain reaction (RT-PCR) and Western blotting were used to examine the expression of maspin.Methylation status of the maspin promoter was detected by methylation-specific polymerase chain reaction (MSP) and bisulphite genomic sequencing.RT-PCR was used to examine the expression of maspin after U87 and U251 cells were treated with 12 (or 15) μmol/L 5-Aza-2-deoxyeytidine (5-Aza-dC) and/or 500 nmol/L trichostatin A (TSA).Results A CpG island in the 5' promoter region of maspin was methylated.Maspin was silenced in human glioma cell lines U87 and U251.The relative intensity of maspin was 0.030 3 ±0.000 6,0.019 6 ±0.001 6,and 0.052 6 ±0.003 4 in 5-Aza-DC,TSA,and 5-Aza-DC plus TSA pretreated U87 cells,and 0.001 2 ± 0.000 1 in control group.The relative intensity of maspin was 0.012 2 ± 0.000 8,0.024 9 ± 0.001 2,and 0.038 4 ± 0.003 1 in 5-Aza-DC,TSA,and 5-Aza-DC plus TSA pretreated U251 cells,and 0.002 2 ± 0.00 3 in control goup.U87,and U251could could be restored by treatment with 5-Aza-DC and/or TSA (P < 0.01).Conclusion Maspin is silenced by promoter methylation and histone modification,and 5-Aza-DC and/or TSA can restore the transcription of maspin in U87 and U251 cells.%目的 探讨乳腺丝氨酸蛋白酶抑制剂基因(maspin)在人胶质瘤细胞株U87、U251中沉默与表观遗传学关系.方法 运用逆转录-聚合酶链反应( RT-PCR)、Western blot检测maspin基因在U87、U251的表达.甲基化特异性聚合酶链反应(MSP)测定maspin基因启动子区区域甲基化状态.同时运用亚硫酸氢盐测序法(BSP)检测maspin基因启动子区甲基化CpG位点.RT-PCR检测12 μmol/L 5-氮杂-2’-脱氧胞苷(5-Aza-DC)和/或500 nmol/L曲古霉素(TSA)、15 μmol/L 5-Aza-DC/或500 nmol/L TSA分别处理U87、U251

  11. Preparation of functionalized nano-graphene oxide particles and their target imaging role in glioma U251 cells%功能化纳米氧化石墨烯微粒的制备及对脑胶质瘤U251细胞的靶向显像作用

    Institute of Scientific and Technical Information of China (English)

    邓跃飞; 李忠军; 庞家栋; 张黎明

    2013-01-01

    Objective To prepare the functionalized nano-graphene oxide (nano-GO) particles,and study their targeted fluorescence imaging effects on glioma U251 cells under near infrared (NIR)irradiation.Methods Single-layer nano-GO particles were obtained after ultrasonic oscillation,and then connected with transferrin (Tf) and fluorescein isothiocyanate (FITC) through poly-L-lysine-G3 to prepare functionalized nano-GO-Tf-FITC particles.Sample morphology was observed and the particle sizes were measured under certain transmission electron microscope (TEM).Glioma U251 cells were employed and divided into targeted experimental group (adding functionalized nano-GO-Tf-FITC particles),parallel control group (adding anti-CD71-FITC reagent) and non-targeted experimental group (adding nano-GO-FITC particles).Fluorescence imaging of these groups was determined by fluorescence microscopy.Results Single-layer particles with a range of 20-100 nm were observed under high TEM,and the functionalized nano-GO-Tf-FITC particles were distributed in a pattern of single layer with a diameter smaller than 100 nm.Yellow green color was monitored in the targeted experimental group and parallel control group,and no imaging was monitored in the non-targeted experimental group because of no transferring integration.Conclusion Functionalized nano-GO-Tf-FITC particles are successfully prepared,and show marked targeted imaging effects on glioma U251 cells.%目的 制备功能化纳米氧化石墨烯(nano-GO-Tf-FITC)微粒,并研究其在近红外线(NIR)照射下对脑胶质瘤U251细胞的靶向荧光显像作用. 方法 将氧化石墨烯(GO)超声振荡制得纳米单层GO(nano-GO)微粒,以聚赖氨酸叠氮基团(poly-L-lysine-G3)连接靶向分子转铁蛋白(Tf)和荧光分子异硫氰酸荧光素(FITC),制备出功能化nano-GO-Tf-FITC微粒,应用透射电镜在一定倍数下观察并拍摄样品形貌,测定样品粒径.将培养的脑胶质瘤U251细胞分成3组,即靶向实验组(加入nano

  12. Effects of Autocrine Motility Factor (AMF) on the Migration and Invasion of Glioblastoma U251 Cells and Their Mechanism%自分泌运动因子AMF对人胶质母细胞瘤U251细胞迁移、侵袭的影响及相关机制研究

    Institute of Scientific and Technical Information of China (English)

    李阳; 汤宁; 刘哲宇; 孙铮

    2016-01-01

    为了探讨自分泌运动因子(autocrine motility factor,AMF)对人胶质母细胞瘤U251细胞迁移、侵袭影响及其相关分子机制,该实验采用了RT-PCR及免疫印迹法检测RNA干扰AMF后U251细胞中AMF的表达变化;细胞划痕实验、Transwell实验分别观察了AMF干扰前后U251细胞迁移、侵袭能力的变化;免疫印记检测AMF干扰前后细胞中总Akt、p-Akt、Sox2、基质金属蛋白酶-2(matrix metalloprotein-2,MMP-2)及MMP-9蛋白水平的变化.研究结果表明,AMF成功干扰后U251细胞的迁移和侵袭能力受到抑制,p-Akt、Sox2、MMP-2和MMP-9蛋白表达水平降低.该研究表明,AMF敲低可以通过下调PI3K/Ak信号通路活性及Sox2、MMP-2和MMP-9蛋白水平,抑制人胶质母细胞瘤U251细胞迁移和侵袭.

  13. Low autocrine interferon beta production as a gene therapy approach for AIDS: Infusion of interferon beta-engineered lymphocytes in macaques chronically infected with SIVmac251

    Directory of Open Access Journals (Sweden)

    Dormont Dominique

    2004-09-01

    Full Text Available Abstract Background The aim of this study was to evaluate gene therapy for AIDS based on the transduction of circulating lymphocytes with a retroviral vector giving low levels of constitutive macaque interferon β production in macaques chronically infected with a pathogenic isolate of SIVmac251. Results Two groups of three animals infected for more than one year with a pathogenic primary isolate of SIVmac251 were included in this study. The macaques received three infusions of their own lymphocytes transduced ex vivo with the construct encoding macaque IFN-β (MaIFN-β or with a vector carrying a version of the MaIFN-β gene with a deletion preventing translation of the mRNA. Cellular or plasma viremia increased transiently following injection in most cases, regardless of the retroviral construct used. Transduced cells were detected only transiently after each infusion, among the peripheral blood mononuclear cells of all the animals, with copy numbers of 10 to 1000 per 106 peripheral mononuclear cells. Conclusion Long-term follow-up indicated that the transitory presence of such a small number of cells producing such small amounts of MaIFN-β did not prevent animals from the progressive decrease in CD4+ cell count typical of infection with simian immunodeficiency virus. These results reveal potential pitfalls for future developments of gene therapy strategies of HIV infection.

  14. Common alleles at 6q25.1 and 1p11.2 are associated with breast cancer risk for BRCA1 and BRCA2 mutation carriers

    DEFF Research Database (Denmark)

    Antoniou, Antonis C; Kartsonaki, Christiana; Sinilnikova, Olga M

    2011-01-01

    Two single nucleotide polymorphisms (SNPs) at 6q25.1, near the ESR1 gene, have been implicated in the susceptibility to breast cancer for Asian (rs2046210) and European women (rs9397435). A genome-wide association study in Europeans identified two further breast cancer susceptibility variants: rs......11249433 at 1p11.2 and rs999737 in RAD51L1 at 14q24.1. Although previously identified breast cancer susceptibility variants have been shown to be associated with breast cancer risk for BRCA1 and BRCA2 mutation carriers, the involvement of these SNPs to breast cancer susceptibility in mutation carriers......% CI: 0.92-1.02, P-trend = 0.20). SNP rs999737 (RAD51L1) was not associated with breast cancer risk for either BRCA1 or BRCA2 mutation carriers (P-trend = 0.27 and 0.30, respectively). The identification of SNPs at 6q25.1 associated with breast cancer risk for BRCA1 mutation carriers will lead...

  15. Down-regulation in multiple human cancers of a novel gene, DMHC, from 17q25.1 that encodes an integral membrane protein.

    Science.gov (United States)

    Mikami, I; Harada, H; Nagai, H; Tsuneizumi, M; Nobe, Y; Koizumi, K; Sugano, S; Tanaka, S; Emi, M

    2001-04-01

    Frequent observations of allelic loss in chromosomal band 17q25.1 in a variety of human cancers have suggested that one or more tumor suppressor genes are present in that region. Moreover, a genetic locus for hereditary focal non-epidermolytic palmoplantar keratoderma, a condition associated with cancer of the esophagus (TOC; Tylosis with Oesophageal Cancer), lies in the same region. We screened cell lines derived from a variety of human cancers by reverse transcription-polymerase chain reaction (RT-PCR) to detect alterations in expression of genes within the region in question, by examining expressed sequence tags located there. These experiments identified an 1834-bp full-length cDNA encoding a novel, 441-amino acid integral membrane protein with seven putative transmembrane domains. This gene showed loss or extreme decrease of expression in 6 of 10 uterine cancer-cell lines, 2 of 11 hepatic cell carcinoma-cell lines, 2 of 7 lung cancer-cell lines, 1 of 6 gastric cancer-cell lines, and 1 of 10 breast cancer-cell lines. (We named it DMHC ("down-regulated in multiple human cancers").) Our results suggest that loss of expression of DMHC at 17q25.1 may play an important role in development of variety of human cancers.

  16. A comparison of the reactivating and therapeutic efficacy of newly developed bispyridinium oximes (K250, K251) with commonly used oximes against tabun in rats and mice.

    Science.gov (United States)

    Kassa, Jiri; Karasova, Jana; Bajgar, Jiri; Kuca, Kamil; Musilek, Kamil; Kopelikova, Irena

    2009-08-01

    The potency of newly developed bispyridinium compounds (K250, K251) in reactivating tabun-inhibited acetylcholinesterase and reducing tabun-induced lethal toxic effects was compared with currently available oximes (obidoxime, trimedoxime, the oxime HI-6) using in vivo methods. Studies determined percentage of reactivation of tabun-inhibited blood and tissue AChE in poisoned rats and showed that the reactivating efficacy of both newly developed oximes is comparable with the oxime HI-6 but it is significantly lower than the reactivating effects of obidoxime and trimedoxime, especially in diaphragm and brain. Both newly developed oximes were also found to be able to slightly reduce lethal toxic effects in tabun-poisoned mice. Their therapeutic efficacy is higher than the potency of the oxime HI-6 but it is lower than the therapeutic effects of trimedoxime and obidoxime. Thus, the reactivating and therapeutic potency of both newly developed oximes (K250, K251) does not prevail over the effectiveness of currently available oximes and, therefore, they are not suitable for their replacement for the treatment of acute tabun poisoning.

  17. CD4+ T-cell loss and delayed expression of modulators of immune responses at mucosal sites of vaccinated macaques following SIV(mac251) infection.

    Science.gov (United States)

    Vaccari, M; Boasso, A; Ma, Z-M; Cecchinato, V; Venzon, D; Doster, M N; Tsai, W P; Shearer, G M; Fuchs, D; Felber, B K; Pavlakis, G N; Miller, C J; Franchini, G

    2008-11-01

    Systemic immunization of macaques with a combination of DNA-poxvirus-based vaccines confers protection from high level of both systemic and mucosal viral replication following rectal exposure to the pathogenic SIV(mac251). Here we investigated early post-infection events in rectal and vaginal tissues, and found that the loss of CCR5+CD4+ T cells was equivalent in vaccinated and control macaques, despite a three logs reduction at mucosal sites of simian immunodeficiency virus (SIV) RNA in the vaccinated group. Even though a normal CD4+ T cell number is not reconstituted at mucosal sites in either group, vaccination appeared to confer a better preservation of the CD4+ CCR5+ T cells that replenish these sites. Analysis of rectal tissues RNA following challenge exposure demonstrated a decreased expression in vaccinated macaques of transforming growth factor-beta, cytotoxic T lymphocyte antigen-4, FoxP3, and indoleamine 2,3-dioxygenase, an immune suppressive enzyme expressed by dendritic cells that converts tryptophan to kynurenine and limits T-cell responses. Accordingly, the ratio of kynurenine and tryptophan in the plasma was significantly reduced in the vaccinated animals respect to the controls. Thus, preexisting adaptive immune responses induced by these vaccine modalities, although they do not protect from CD4+ T-cell depletion, nevertheless, they contain SIV(mac251) replication and delay expression of markers of T-cell activation and/or suppression at mucosal sites.

  18. H{sub 2} reduction of surface oxides on Pd-based membrane model systems – The case of Pd(1 0 0) and Pd{sub 75}Ag{sub 25}(1 0 0)

    Energy Technology Data Exchange (ETDEWEB)

    Fernandes, V.R. [Department of Physics, Norwegian University of Science and Technology, NO-7491 Trondheim (Norway); Gustafson, J. [Division of Synchrotron Radiation Research, Lund University, Box 117, SE-221 00 Lund (Sweden); Farstad, M.H.; Walle, L.E. [Department of Physics, Norwegian University of Science and Technology, NO-7491 Trondheim (Norway); Blomberg, S.; Lundgren, E. [Division of Synchrotron Radiation Research, Lund University, Box 117, SE-221 00 Lund (Sweden); Venvik, H.J. [Department of Chemical Engineering, Norwegian University of Science and Technology, NO-7491 Trondheim (Norway); Borg, A., E-mail: anne.borg@ntnu.no [Department of Physics, Norwegian University of Science and Technology, NO-7491 Trondheim (Norway)

    2014-09-15

    Highlights: • H{sub 2} reduction of the surface oxide is significantly slower for Pd{sub 75}Ag{sub 25}(1 0 0) compared to Pd(1 0 0). • The reduction behavior shows complex temperature dependence, not well described by Avrami kinetics. • Oxygen spillover effects during the surface oxide reduction are observed for Pd(1 0 0). • For Pd(1 0 0) the observed reduction rate is rather independent of temperature. • For Pd{sub 75}Ag{sub 25}(1 0 0) the reduction rate displays a non-monotonic variation with temperature. - Abstract: Reduction of the (√(5)×√(5))R27° surface oxide on Pd(1 0 0) and Pd{sub 75}Ag{sub 25}(1 0 0) surfaces by H{sub 2} has been studied using high-resolution photoelectron spectroscopy in situ at H{sub 2} pressures 5 × 10{sup −9} mbar and 5 × 10{sup −8} mbar and selected temperatures in the range 30 °C to 170 °C. The reduction is slower on Pd{sub 75}Ag{sub 25}(1 0 0) compared to Pd(1 0 0) for all temperatures and pressures investigated. For Pd(1 0 0), the surface oxide reduction rate is rather independent of temperature, while for Pd{sub 75}Ag{sub 25}(1 0 0) a non-monotonic variation is observed. As indicated by kinetic analysis, the complex reduction behavior is not well described by Avrami kinetics. Oxygen spillover effects contribute to this picture for Pd(1 0 0), while surface compositional effects appear to dominate the performance for Pd{sub 75}Ag{sub 25}(1 0 0). These findings may have implications for understanding the oxidation, reduction and hydrogen transport properties of Pd–Ag membranes.

  19. Synthesis of tetrahydrohonokiol derivates and their evaluation for cytotoxic activity against CCRF-CEM leukemia, U251 glioblastoma and HCT-116 colon cancer cells.

    Science.gov (United States)

    Bernaskova, Marketa; Kretschmer, Nadine; Schuehly, Wolfgang; Huefner, Antje; Weis, Robert; Bauer, Rudolf

    2014-01-20

    Biphenyl neolignans such as honokiol and magnolol, which are the major active constituents of the Asian medicinal plant Magnolia officinalis, are known to exert a multitude of pharmacological and biological activities. Among these, cytotoxic and tumor growth inhibitory activity against various tumour cell lines are well-documented. To further elucidate the cytotoxic effects of honokiol derivatives, derivatizations were performed using tetrahydrohonokiol as a scaffold. The derivatizations comprised the introduction of functional groups, e.g., nitro and amino groups, as well as alkylation. This way, 18 derivatives, of which 13 were previously undescribed compounds, were evaluated against CCRF-CEM leukemia cells, U251 glioblastoma and HCT-116 colon cancer cells. The results revealed no significant cytotoxic effects in any of the three tested cell lines at a test concentration of 10 µM.

  20. Synthesis of Tetrahydrohonokiol Derivates and Their Evaluation for Cytotoxic Activity against CCRF-CEM Leukemia, U251 Glioblastoma and HCT-116 Colon Cancer Cells

    Directory of Open Access Journals (Sweden)

    Marketa Bernaskova

    2014-01-01

    Full Text Available Biphenyl neolignans such as honokiol and magnolol, which are the major active constituents of the Asian medicinal plant Magnolia officinalis, are known to exert a multitude of pharmacological and biological activities. Among these, cytotoxic and tumor growth inhibitory activity against various tumour cell lines are well-documented. To further elucidate the cytotoxic effects of honokiol derivatives, derivatizations were performed using tetrahydrohonokiol as a scaffold. The derivatizations comprised the introduction of functional groups, e.g., nitro and amino groups, as well as alkylation. This way, 18 derivatives, of which 13 were previously undescribed compounds, were evaluated against CCRF-CEM leukemia cells, U251 glioblastoma and HCT-116 colon cancer cells. The results revealed no significant cytotoxic effects in any of the three tested cell lines at a test concentration of 10 µM.

  1. Noradrenaline increases intracellular glutathione in human astrocytoma U-251 MG cells by inducing glutamate-cysteine ligase protein via β3-adrenoceptor stimulation.

    Science.gov (United States)

    Yoshioka, Yasuhiro; Kadoi, Hisatsugu; Yamamuro, Akiko; Ishimaru, Yuki; Maeda, Sadaaki

    2016-02-05

    Glutathione (GSH) plays a critical role in protecting cells from oxidative damage. Since neurons rely on the supply of GSH from astrocytes to maintain optimal intracellular GSH concentrations, the GSH concentration of astrocytes is important for the survival of neighboring neurons against oxidative stress. The neurotransmitter noradrenaline is known to modulate the functions of astrocytes and has been suggested to have neuroprotective properties in neurodegenerative diseases. To elucidate the mechanisms underlying the neuroprotective properties of noradrenaline, in this study, we investigated the effect of noradrenaline on the concentrations of intracellular GSH in human U-251 malignant glioma (MG; astrocytoma) cells. Treatment of the cells with noradrenaline for 24h concentration-dependently increased their intracellular GSH concentration. This increase was inhibited by a non-selective β-adrenoceptor antagonist propranolol and by a selective β3-adrenoceptor antagonist SR59230A, but not by a non-selective α-adrenoceptor antagonist phenoxybenzamine, or by a selective β1-adrenoceptor antagonist atenolol or by a selective β2-adrenoceptor antagonist butoxamine. In addition, the selective β3-adrenoceptor agonist CL316243 increased the intracellular GSH in U-251 MG cells. Treatment of the cells with noradrenaline (10μM) for 24h increased the protein level of the catalytic subunit of glutamate-cysteine ligase (GCLc), the rate-limiting enzyme of GSH synthesis; and this increase was inhibited by SR59230A. These results thus suggest that noradrenaline increased the GSH concentration in astrocytes by inducing GCLc protein in them via β3-adrenoceptor stimulation.

  2. Cannabinoid type 1 receptor ligands WIN 55,212-2 and AM 251 alter anxiety-like behaviors of marmoset monkeys in an open-field test.

    Science.gov (United States)

    Cagni, Priscila; Barros, Marilia

    2013-03-01

    Cannabinoid type 1 receptors (CB1r) are an important modulatory site for emotional behavior. However, little is known on the effects of CB1r ligands on emotionality aspects of primates, even with their highly similar behavioral response and receptor density/distribution as humans. Thus, we analyzed the effects of the CB1r agonist WIN 55,212-2 (WIN; 1mg/kg) and the antagonist AM 251 (AM; 2mg/kg), systemically administered prior to a single brief (15 min) exposure to a novel open-field (OF) environment, on the behavior of individually tested adult black tufted-ear marmosets. Both WIN- and AM-treated subjects, compared to vehicle controls, had significantly lower rates of long (contact) calls and exploration, while higher levels of vigilance-related behaviors (scan/glance); these are indicators of anxiolysis in this setup. Changes in locomotion were not detected. However, in the vehicle and AM-groups, sojourn in the peripheral zone of the OF was significantly higher than in its central region. WIN-treated marmosets spent an equivalent amount of time in both zones. Therefore, activation or blockade CB1r function prior to a short and individual exposure to an unfamiliar environment exerted a significant and complex influence on different behavioral indicators of anxiety in these monkeys (i.e., a partially overlapping anxiolytic-like profile). AM 251, however, has no anxiolytic effect when the time spent in the center of the OF is considered. This is a major difference when compared to the WIN-treated group. Data were compared to the response profile reported in other pre-clinical (rodent) and clinical studies.

  3. A variant of the H6 homeobox gene maps to the 10q25.1-q26.2 region of human chromosome 10

    Energy Technology Data Exchange (ETDEWEB)

    Stadler, H.S.; Solursh, M. [Univ. of Iowa, Iowa City, IA (United States); Goodfellow, P.J. [Washington Univ. School of Medicine, St. Louis, MO (United States)] [and others

    1994-09-01

    Homeobox genes represent a class of transcription factors which play a major role in the regulation of embryogenesis. Utilizing primers based on the conserved regions of the H6 homeobox gene, we were able to amplify several H6-like homeobox sequences from human genomic DNA. Analysis of these sequences indicated that at least 3 distinct H6-like homeobox regions may be present in Homo sapiens. From these distinct homeobox regions, primers were selected to screen a CEPH mega-YAC library for genomic clones containing the variant H6-like sequences. These screenings identified a YAC clone (YH6var) which contained the variant H6-like homeobox sequence. Utilizing a human/rodent somatic cell panel (Coriell B), the end clones from YH6var were chromosomally mapped to human chromosomes 10 and 16, indicating that YH6var was chimeric. An additional sequence flanking the H6 variant homeobox region was derived by inverse-PCR using YH6var as a template. From this flanking sequence, Homo sapien-specific primers were selected to assign the H6 variant to human chromosome 10 using the same Coriell B panel. The H6 variant gene was sublocalized to the 10q25.1-q26.2 region using a panel of somatic cell hybrids that retain well-characterized deletions/derivatives of chromosome 10. We are currently screening for STS-based markers for genetic linkage studies to confirm our physical mapping to the 10q25.1-q26.2 region as well as for use in linkage studies with developmental defects mapping to the same chromosomal region.

  4. Effects of cannabinoid receptor 1 antagonist AM251 on vasoreactivity in hemorrhagic shock rats%大麻素受体1拮抗剂AM251对失血性休克大鼠血管反应性的影响

    Institute of Scientific and Technical Information of China (English)

    李楠; 高娜; 王静; 侯立朝; 高燕

    2012-01-01

    目的 拟观察大麻素受体1(CB1R)在失血性休克大鼠腹主动脉和肠系膜上动脉的表达变化情况,及大麻素CB1受体拮抗剂AM251对血管反应性的影响.方法 将SD大鼠随机分为假手术(Sham)组和失血性休克(HS)组.检测休克动物血管反应性的变化,以及动脉血管大麻素CB1受体mRNA和蛋白表达情况;观察CB1受体拮抗剂AM251对休克后血管低反应性及低血压的影响.结果 HS组动物均发生血管低反应性,腹主动脉和肠系膜上动脉2~3级分支动脉血管组织CB1受体mRNA和蛋白均呈阳性表达;CB1受体拮抗剂AM251能显著提高休克后血管低反应性,并可明显改善休克后的低血压.结论 大麻素CB1受体与大鼠失血性休克后血管低反应性密切相关,其拮抗剂具有抗重度失血性休克作用.

  5. Replication and functional genomic analyses of the breast cancer susceptibility locus at 6q25.1 generalize its importance in women of chinese, Japanese, and European ancestry.

    Science.gov (United States)

    Cai, Qiuyin; Wen, Wanqing; Qu, Shimian; Li, Guoliang; Egan, Kathleen M; Chen, Kexin; Deming, Sandra L; Shen, Hongbing; Shen, Chen-Yang; Gammon, Marilie D; Blot, William J; Matsuo, Keitaro; Haiman, Christopher A; Khoo, Ui Soon; Iwasaki, Motoki; Santella, Regina M; Zhang, Lina; Fair, Alecia Malin; Hu, Zhibin; Wu, Pei-Ei; Signorello, Lisa B; Titus-Ernstoff, Linda; Tajima, Kazuo; Henderson, Brian E; Chan, Kelvin Y K; Kasuga, Yoshio; Newcomb, Polly A; Zheng, Hong; Cui, Yong; Wang, Furu; Shieh, Ya-Lan; Iwata, Hiroji; Le Marchand, Loic; Chan, Sum Yin; Shrubsole, Martha J; Trentham-Dietz, Amy; Tsugane, Shoichiro; Garcia-Closas, Montserrat; Long, Jirong; Li, Chun; Shi, Jiajun; Huang, Bo; Xiang, Yong-Bing; Gao, Yu-Tang; Lu, Wei; Shu, Xiao-Ou; Zheng, Wei

    2011-02-15

    We evaluated the generalizability of a single nucleotide polymorphism (SNP), rs2046210 (A/G allele), associated with breast cancer risk that was initially identified at 6q25.1 in a genome-wide association study conducted among Chinese women. In a pooled analysis of more than 31,000 women of East-Asian, European, and African ancestry, we found a positive association for rs2046210 and breast cancer risk in Chinese women [ORs (95% CI) = 1.30 (1.22-1.38) and 1.64 (1.50-1.80) for the AG and AA genotypes, respectively, P for trend = 1.54 × 10⁻³⁰], Japanese women [ORs (95% CI) = 1.31 (1.13-1.52) and 1.37 (1.06-1.76), P for trend = 2.51 × 10⁻⁴], and European-ancestry American women [ORs (95% CI) = 1.07 (0.99-1.16) and 1.18 (1.04-1.34), P for trend = 0.0069]. No association with this SNP, however, was observed in African American women [ORs (95% CI) = 0.81 (0.63-1.06) and 0.85 (0.65-1.11) for the AG and AA genotypes, respectively, P for trend = 0.4027]. In vitro functional genomic studies identified a putative functional variant, rs6913578. This SNP is 1,440 bp downstream of rs2046210 and is in high linkage disequilibrium with rs2046210 in Chinese (r(2) = 0.91) and European-ancestry (r² = 0.83) populations, but not in Africans (r² = 0.57). SNP rs6913578 was found to be associated with breast cancer risk in Chinese and European-ancestry American women. After adjusting for rs2046210, the association of rs6913578 with breast cancer risk in African Americans approached borderline significance. Results from this large consortium study confirmed the association of rs2046210 with breast cancer risk among women of Chinese, Japanese, and European ancestry. This association may be explained in part by a putatively functional variant (rs6913578) identified in the region.

  6. Cisplatin Resistance Inducing and Autophagy in Glioma Cell Line U251%顺铂耐受胶质瘤细胞株诱导和细胞自噬的观察

    Institute of Scientific and Technical Information of China (English)

    付军; 陈芙蓉; 陈忠平

    2007-01-01

    背景与目的:明确胶质瘤耐药相关分子机制,有助于寻找新的治疗对策.我们将诱导对顺铂耐药的脑胶质瘤细胞株,探讨细胞自噬和胶质瘤细胞顺铂耐受的关系.方法:应用人胶质瘤细胞株U251,采用顺铂剂量梯度爬升筛选方法,诱导耐药胶质瘤细胞株;用MTT法测定该耐药细胞对顺铂的半数抑制浓度(IC50);电镜和GFP-LC3荧光染色检测细胞内自噬体;AnnexinV-FITC染色检测细胞凋亡;Western blot检测自噬相关蛋白LC3、Beclin 1以及凋亡分子Caspase-3水平.结果:我们诱导获得的耐药胶质瘤细胞株U251/CP2对顺铂半数抑制浓度(IC50)较亲代U251增加了62.7倍(分别是1.2±0.4 ìg/ml和76.5±22.5 μg/ml).电镜和GFP-LC3荧光染色观察表明胶质瘤细胞呈现典型的自噬特征,表现为电镜下广泛的自噬性空泡的出现和GFP-LC3斑点状分布,且能够被自噬抑制剂3-MA和ERK抑制剂PD98059抑制.凋亡检测表明,经顺铂处理后,U251/CP2较U251凋亡率低,3-MA和PD98059能够显著增强顺铂对U251/CP2的凋亡诱导作用.Western blot检测表明,U251/CP2较U251具有显著上调的LC3和Beclin 1水平,而Caspase-3水平较低.结论:U251胶质瘤细胞能够通过自噬作用保护细胞免受化疗药物顺铂的杀伤,从而免于发生细胞凋亡.

  7. Association of a single nucleotide polymorphism at 6q25.1,rs2046210, with endometrial cancer risk among Chinese women

    Institute of Scientific and Technical Information of China (English)

    Guoliang Li; Qiuyin Cai; Yong-Bing Xiang; Regina Courtney; Jia-Rong Cheng; Bo Huang; Ji-Rong Long; Hui Cai; Wei Zheng; Xiao-Ou Shu

    2011-01-01

    A recent genome-wide association study identified a new susceptibility locus for breast cancer, rs2046210, which is a single nucleotide polymorphism (SNP) located upstream of the estrogen receptor α (ESR1) gene on chromosome 6q25.1. Given that endometrial cancer shares many risk factors with breast cancer and both are related to estrogen exposure and that rs2046210 is in close proximity to the ESR1 gene, we evaluated the association of SNP rs2046210 with endometrial cancer risk among 953 cases and 947 controls in a population-based, case-control study conducted in Shanghai, China. Logistic regression models were used to derive odds ratios (ORs) and 95% confidence intervals (95% Cis) after adjusting for potential confounders. We found that the A allele of rs2046210, linked to an increased risk of breast cancer, was associated with increased but not statistically significant risk of endometrial cancer (OR = 1.16, 95% CI = 0.96-1.41 for the GA and AA genotypes compared with the GG genotype); the association was stronger among post-menopausal women (OR = 1.28, 95% CI = 1.00-1.65). The association tended to be stronger among women with higher or longer estrogen exposure than among women with relatively lower or shorter exposure to estrogen. Our study suggests that rs2046210 may play a role in the etiology of endometrial cancer. Additional studies are needed to confirm our findings.

  8. Low dose rectal inoculation of rhesus macaques by SIV SME660 or SIV MAC251 recapitulates human mucosal infection by HIV-1

    Energy Technology Data Exchange (ETDEWEB)

    Koraber, Bette [Los Alamos National Laboratory; Perelson, Alan [Los Alamos National Laboratory; Hraber, Peter [Los Alamos National Laboratory; Giorgi, E [Los Alamos National Laboratory; Bhattacharya, T [Los Alamos National Laboratory

    2009-01-01

    Recently, we developed a novel approach to the identification of transmitted or early founder HIV -1 genomes in acutely infected humans based on single genome amplification and sequencing. Here we tested this approach in 18 acutely infected Indian rhesus macaques to determine the molecular features of SIV transmission. Animals were inoculated intrarectally (IR) or intravenously (IV) with stocks of SIVmac251 or SIVsmE660 that exhibited sequence diversity typical of early-chronic HIV -1 infection. 987 full-length SIV env sequences (median of 48 per animal) were determined from plasma virion RNA one to five weeks after infection. IR inoculation was followed by productive infection by one or few viruses (median 1; range 1-5) that diversified randomly with near star-like phylogeny and a Poisson distribution of mutations. Consensus viral sequences from ramp-up and peak viremia were identical to viruses found in the inocula or differed from them by only one or few nuc1eotides, providing direct evidence that early plasma viral sequences coalesce to transmitted/founder virus( es). IV infection was approximately 10,000-fold more efficient than IR infection, and viruses transmitted by either route represented the full genetic spectra of the inocula. These findings identify key similarities in mucosal transmission and early diversification between SIV and HIV -1.

  9. Elevation of Ser9 phosphorylation of GSK3β is required for HERV-W env-mediated BDNF signaling in human U251 cells.

    Science.gov (United States)

    Qin, Chengchen; Li, Shan; Yan, Qiujin; Wang, Xiuling; Chen, Yatang; Zhou, Ping; Lu, Mengxin; Zhu, Fan

    2016-08-03

    Human endogenous retrovirus W family (HERV-W) envelope (env) is known to be associated with neurological and psychiatric disorders, such as multiple sclerosis and schizophrenia. Previous studies showed that overexpression of HERV-W env could induce brain-derived neurotrophic factor (BDNF) gene expression. In human and rat cells, BDNF-mediated signal transduction might be modulated by glycogen synthase kinase 3β (GSK3β). Both BDNF and GSK3β are schizophrenia-related genes. In this paper, we investigated whether GSK3β was involved in the HERV-W env-induced expression of BDNF. We found that HERV-W env increased phosphorylation of GSK3β at Ser9 (p-GSK3β (Ser9)) and the ratio of p-GSK3β (Ser9) to total GSK3β (pW env led to a 36.2% reduction in GSK3β activity compared to control (pW env might activate the GSK3β signaling pathway in U251 cells. Further, knockdown of GSK3β reduced the expression of total GSK3β, p-GSK3β (Ser9), and the ratio of p-GSK3β (Ser9) to total GSK3β by 28.6%, 50.4%, and 30.2%, respectively (pW env-induced BDNF expression, and will hopefully improve our understanding of the role of HERV-W env in neurological and psychiatric diseases (schizophrenia, etc).

  10. The SNP rs931794 in 15q25.1 Is Associated with Lung Cancer Risk: A Hospital-Based Case-Control Study and Meta-Analysis.

    Directory of Open Access Journals (Sweden)

    Qi Wang

    Full Text Available Lung cancer is one of the most common human malignant diseases and the leading cause of cancer death worldwide. The rs931794, a SNP located in 15q25.1, has been suggested to be associated with lung cancer risk. Nevertheless, several genetic association studies yielded controversial results.A hospital-based case-control study involving 611 cases and 1062 controls revealed the variant of rs931794 was related to increased lung cancer risk. Stratified analyses revealed the G allele was significantly associated with lung cancer risk among smokers. Following meta-analysis including 6616 cases and 7697 controls confirmed the relevance of rs931794 variant with increased lung cancer risk once again. Heterogeneity should be taken into account when interpreting the consequences. Stratified analysis found ethnicity, histological type and genotyping method were not the sources of between-study heterogeneity. Further sensitivity analysis revealed that the study "Hsiung et al (2010" might be the major contributor to heterogeneity. Cumulative meta-analysis showed the trend was increasingly obvious with adding studies, confirming the significant association.Results from our current case-control study and meta-analysis offered insight of association between rs931794 and lung cancer risk, suggesting the variant of rs931794 might be related with increased lung cancer risk.

  11. Down-regulation in human cancers of DRHC, a novel helicase-like gene from 17q25.1 that inhibits cell growth.

    Science.gov (United States)

    Nagai, H; Yabe, A; Mine, N; Mikami, I; Fujiwara, H; Terada, Y; Hirano, A; Tsuneizumi, M; Yokota, T; Emi, M

    2003-04-10

    Frequent observations of allelic loss in chromosomal band 17q25.1 in a variety of human cancers have suggested that one or more tumor suppressor genes are normally present in this region. Moreover, a locus responsible for hereditary focal non-epidermolytic palmoplantar keratoderma (tylosis oesophageal cancer; TOC), a condition associated with esophageal cancer, has been mapped to the same band. During efforts to sequence, by shot-gun methods, a 1 Mb target region that we had defined as the DNA segment harboring the putative tumor suppressor gene(s) involved in these events, we identified a novel cDNA, DRHC (down-regulated in human cancers), that showed reduced expression in 28 of 95 (29%) cell lines derived from a variety of human cancers. The full-length cDNA, 6275 bp long, was expressed predominantly in thymus and brain. The predicted 1942-amino-acid product exhibited significant sequence homology to yeast enzymes belonging to the DEAD-helicase superfamily, and appeared to be a Uvr/Rep helicase with a DEXDc consensus domain. Transfection of a DRHC expression vector inhibited growth of cancer cells in liquid medium or soft agar. The results suggest that loss of expression of DRHC may play a role in human carcinogenesis.

  12. 辽东湾地区锦州25-1大型轻质油气田成藏条件与成藏过程%Hydrocarbon accumulation conditions and process of Jinzhou 25-1 large-scale light gas-oil field in Liaodong Bay

    Institute of Scientific and Technical Information of China (English)

    田立新; 徐长贵; 江尚昆

    2011-01-01

    以三维地震资料、钻井资料分析以及流体包裹体资料为基础,分析锦州25-1大型轻质油气田基本成藏条件,并对其生烃史、排烃动力、成藏期次、油气充注过程进行研究.结果表明:锦州25-1油气田是一个优质的大型油气田,其形成的基本条件是该区具有多成因的大型圈闭群、充足的烃源岩条件以及理想的储盖组合;烃原岩排烃的主要动力是干酪根向油转化所产生的孔隙流体压力,孔隙流体压力的周期性累积和释放是油气幕式充注的主要原因;油气大规模充注成藏的时期是东营组末期(21~25 Ma);油气主要来自锦州25-1构造南北两翼的辽西北洼和辽西中洼;双灶供烃、超压-持续强充注、高效输导、理想储盖4因素耦合是研究区形成大型优质油气田的根本原因.%Based on 3D seismic data, drilling and fluid inclusion data, Jinzhou 25-1 large-scale oil-gas field formation conditions were analyzed. The hydrocarbon generation history, hydrocarbon expulsion dynamics, hydrocarbon accumulation period and hydrocarbon charging history of the field were researched. The results show that Jinzhou 2S-1 oil-gas field is a perfect large-scale light gas-oil field and its basic formation conditions include multiple genesis large-scale trap group, abundant hydrocarbon source rock and perfect reservoir-cap combination. The hydrocarbon expulsion dynamic in the source kitchen is the pore fluid pressure produced by the transformation from kerogen to hydrocarbon. The episodic accumulation and release of the pore fluid pressure is the main reason of the hydrocarbon episodic charging. The main pool forming period of the field is lateral period (21 -25 Ma) of Dongying group deposition. The hydrocarbon source of the oil-gas field is from the north sub-sags and middle sub-sag of Iiaoxi sag. The major reason of the large-scale gas-oil field formation in the area is the matching of the hydrocarbon supplying

  13. Influence of intracerebroventricular or intraperitoneal administration of cannabinoid receptor agonist (WIN 55,212-2) and inverse agonist (AM 251) on the regulation of food intake and hypothalamic serotonin levels.

    Science.gov (United States)

    Merroun, Ikram; Errami, Mohammed; Hoddah, Hanaa; Urbano, Gloria; Porres, Jesús M; Aranda, Pilar; Llopis, Juan; López-Jurado, María

    2009-05-01

    The effect of intracerebroventricular or intraperitoneal administration of cannabinoid receptor agonist WIN 55,212-2 or inverse agonist AM 251 on food intake and extracellular levels of serotonin and acetic acid 5-hydroxy-indol from presatiated rats was studied. Compared to the vehicle-injected control, the intracerebroventricular administration of WIN 55,212-2 was associated with a significant increase in food intake, whereas the administration of AM 251 caused a significant reduction in this respect. These results were accompanied by considerable reductions or increases in serotonin and acetic acid 5-hydroxy-indol levels compared to the vehicle-injected control and the baseline values for the different experimental groups studied. Intraperitoneal administration of WIN 55,212-2 at doses of 1 and 2 mg/kg promoted hyperphagia up to 6 h after injection, whereas administration of a higher dose (5 mg/kg) significantly inhibited food intake and motor behaviour in partially satiated rats. Administration of any of the AM 251 doses studied (0.5, 1, 2, 5 mg/kg) led to a significant decrease in the amount of food ingested from 2 h after the injection, compared to the vehicle-injected control group, with the most striking effect being observed when the 5 mg/kg dose was injected.

  14. Variants located upstream of CHRNB4 on chromosome 15q25.1 are associated with age at onset of daily smoking and habitual smoking.

    Directory of Open Access Journals (Sweden)

    Manav Kapoor

    Full Text Available Several genome-wide association and candidate gene studies have linked chromosome 15q24-q25.1 (a region including the CHRNA5-CHRNA3-CHRNB4 gene cluster with alcohol dependence, nicotine dependence and smoking-related illnesses such as lung cancer and chronic obstructive pulmonary disease. To further examine the impact of these genes on the development of substance use disorders, we tested whether variants within and flanking the CHRNA5-CHRNA3-CHRNB4 gene cluster affect the transition to daily smoking (individuals who smoked cigarettes 4 or more days per week in a cross sectional sample of adolescents and young adults from the COGA (Collaborative Study of the Genetics of Alcoholism families. Subjects were recruited from families affected with alcoholism (either as a first or second degree relative and the comparison families. Participants completed the SSAGA interview, a comprehensive assessment of alcohol and other substance use and related behaviors. Using the Quantitative trait disequilibrium test (QTDT significant association was detected between age at onset of daily smoking and variants located upstream of CHRNB4. Multivariate analysis using a Cox proportional hazards model further revealed that these variants significantly predict the age at onset of habitual smoking among daily smokers. These variants were not in high linkage disequilibrium (0.28

  15. Location and dynamics of the immunodominant CD8 T cell response to SIVΔnef immunization and SIVmac251 vaginal challenge.

    Directory of Open Access Journals (Sweden)

    Arun K Sasikala-Appukuttan

    Full Text Available Live-attenuated SIV vaccines (LAVs have been the most effective to date in preventing or partially controlling infection by wild-type SIV in non-human primate models of HIV-1 transmission to women acting by mechanisms of protection that are not well understood. To gain insights into mechanisms of protection by LAVs that could aid development of effective vaccines to prevent HIV-1 transmission to women, we used in situ tetramer staining to determine whether increased densities or changes in the local distribution of SIV-specific CD8 T cells correlated with the maturation of SIVΔnef vaccine-induced protection prior to and after intra-vaginal challenge with wild-type SIVmac251. We evaluated the immunodominant Mamu-A1*001:01/Gag (CM9 and Mamu-A1*001:01/Tat (SL8 epitope response in genital and lymphoid tissues, and found that tetramer+ cells were present at all time points examined. In the cervical vaginal tissues, most tetramer+ cells were distributed diffusely throughout the lamina propria or co-localized with other CD8 T cells within lymphoid aggregates. The distribution and densities of the tetramer+ cells at the portal of entry did not correlate with the maturation of protection or change after challenge. Given these findings, we discuss the possibility that changes in other aspects of the immune system, including the quality of the resident population of virus-specific effector CD8 T cells could contribute to maturation of protection, as well as the potential for vaccine strategies that further increase the size and quality of this effector population to prevent HIV-1 transmission.

  16. ADCC develops over time during persistent infection with live-attenuated SIV and is associated with complete protection against SIV(mac251 challenge.

    Directory of Open Access Journals (Sweden)

    Michael D Alpert

    Full Text Available Live-attenuated strains of simian immunodeficiency virus (SIV routinely confer apparent sterilizing immunity against pathogenic SIV challenge in rhesus macaques. Understanding the mechanisms of protection by live-attenuated SIV may provide important insights into the immune responses needed for protection against HIV-1. Here we investigated the development of antibodies that are functional against neutralization-resistant SIV challenge strains, and tested the hypothesis that these antibodies are associated with protection. In the absence of detectable neutralizing antibodies, Env-specific antibody-dependent cell-mediated cytotoxicity (ADCC emerged by three weeks after inoculation with SIVΔnef, increased progressively over time, and was proportional to SIVΔnef replication. Persistent infection with SIVΔnef elicited significantly higher ADCC titers than immunization with a non-persistent SIV strain that is limited to a single cycle of infection. ADCC titers were higher against viruses matched to the vaccine strain in Env, but were measurable against viruses expressing heterologous Env proteins. In two separate experiments, which took advantage of either the strain-specificity or the time-dependent maturation of immunity to overcome complete protection against SIV(mac251 challenge, measures of ADCC activity were higher among the SIVΔnef-inoculated macaques that remained uninfected than among those that became infected. These observations show that features of the antibody response elicited by SIVΔnef are consistent with hallmarks of protection by live-attenuated SIV, and reveal an association between Env-specific antibodies that direct ADCC and apparent sterilizing protection by SIVΔnef.

  17. Assessment of the toll-like receptor 4 Asp299Gly, Thr399Ile and interleukin-8 -251 polymorphisms in the risk for the development of distal gastric cancer

    Directory of Open Access Journals (Sweden)

    Maldonado-Garza Hector J

    2007-04-01

    Full Text Available Abstract Background The intensity of the inflammation induced by Helicobacter pylori colonization is associated with the development of distal gastric cancer (GC. The host response to H. pylori has been related to genetic polymorphisms that influence both innate and adaptive immune responses. Our aim was to investigate whether the presence of the TLR4 Asp299Gly, TLR4 Thr399Ile and IL-8-251 A/T polymorphisms had any influence in the development of distal GC in a Mexican population. Methods We studied 337 patients that were divided in two groups: 78 patients with histologically confirmed distal GC and 259 non-cancer controls. The presence of H. pylori in the control population was defined by positive results of at least two of four diagnostic tests: serology, histology, rapid urease test and culture. Human DNA was purified and genotyped for TLR4 Asp299Gly polymorphism by pyrosequencing, for TLR4 Thr399Ile by PCR-RFLP and for IL8-251 by the amplification refractory mutation system (ARMS-PCR. Results The non-cancer control group was found to be in Hardy-Weinberg equilibrium at the polymorphic loci studied (chi-square H-W = 0.58 for IL8-251, 0.42 for TLR4 Asp299Gly and 0.17 for TLR4 Thr399Ile. The frequencies of mutated alleles (homozygous plus heterozygous were compared between cases and controls. We found no significant difference for TLR4- Asp299Gly [the 7.7% of distal GC patients and 7.7 % non-cancer controls (p = 0.82] and for TLR4 Thr399Ile [the 1.3% of GC patients and the 5% of the control population (p = 0.2]. In contrast, for IL-8-251 A/T, 80.77% of the GC patients and 66.4% in the control group age and gender matched had at least one copy of mutated allele (OR = 2.12, 95% CI = 1.1–4.2 (p = 0.023. Conclusion This study showed that the IL8-251*A allele could be related to the development of distal gastric cancer in this Mexican population.

  18. Effects of VE-statin/Egfl7 gene silencing on gene expression profiles of malignant glioma cell U251%VE-statin/Egfl7基因沉默对恶性胶质瘤细胞U251基因表达谱的影响

    Institute of Scientific and Technical Information of China (English)

    黄纯海; 田志; 万一; 张晶晶

    2013-01-01

    目的 探讨VE-statin/Egfl7基因在恶性胶质瘤中表达的分子调控机制.方法 用小RNA干扰技术沉默VE-statin/Egfl7基因在人恶性胶质瘤U251细胞中的表达,然后用基因表达谱芯片研究VE-statin/Egfl7基因沉默前后该细胞基因表达谱的变化,进行生物信息学分析.并选取两条差异表达基因用实时荧光定量PCR进行验证.结果 通过RNA干扰技术明显抑制了VE-statin/Egfl7基因在U251细胞中的表达.基因表达芯片检测结果发现与对照组相比,实验组下调基因有141条,包括未知功能基因7条,已知功能基因134条;上调的基因有130条,包括未知功能基因3条,已知功能基因127条.通过生物信息学分析发现这些基因主要与细胞增殖分化、凋亡及细胞外基质黏附等生物学功能密切相关,涉及的主要信号通路包括表皮生长因子受体ERBB家族信号、细胞存活信号及整合素αvβ3信号.结论 VE-statin/Egfl7在恶性胶质瘤中的分子调控机制可能通过PBK/Akt和Ras/MAPK两条信号转导通路实现,为进一步研究VE-statin/Egfl7的功能和分子机制提供了新的线索.

  19. 14-3-3和CLIC4蛋白在U251细胞自噬中的相互作用%The interaction of 14-3-3 and CLIC4 proteins in the autophagy of U251 cells

    Institute of Scientific and Technical Information of China (English)

    袁兆新; 金笛; 张宏宇

    2015-01-01

    目的 通过饥饿诱导神经胶质瘤U251细胞发生自噬,探讨细胞CLIC4和14-3-3蛋白在饥饿条件下诱导自噬过程中的相互作用.方法 通过Hoechst、14-3-3 epsilon、CLIC4染色于共聚焦显微镜下观察抑制CLIC4表达对于饥饿条件下,14-3-3 epsilon蛋白与CLIC4共定位的影响.通过Western Blot技术检测Beclin 1及14-3-3蛋白表达.免疫共沉淀技术检测14-3-3 epsilon蛋白与CLIC4蛋白的结合水平.结果 共聚焦显微镜观察14-3-3 epsilon和CLIC4荧光染色结果显示,饥饿条件下,14-3-3 epsilon蛋白与CLIC4共定位显著增加,并广泛分布于胞浆及细胞核中.同时Westem Blot结果表明抑制CLIC4表达能够引起14-3-3蛋白以及自噬相关蛋白Beclin1表达增加.饥饿条件下,14-3-3 epsilon蛋白与CLIC4共沉淀增强,而抑制CLIC4表达能够降低两者结合水平.结论 14-3-3epsilon蛋白与CLIC4的相互作用由于RNA干扰而减弱,促进了14-3-3蛋白水平上调,进而增强了14-3-3蛋白对Beclin1信号通路的调节,引起Beclin1表达增加,进一步激活饥饿条件下U251细胞自噬过程.

  20. Multiple independent loci at chromosome 15q25.1 affect smoking quantity: a meta-analysis and comparison with lung cancer and COPD.

    Directory of Open Access Journals (Sweden)

    Nancy L Saccone

    2010-08-01

    Full Text Available Recently, genetic association findings for nicotine dependence, smoking behavior, and smoking-related diseases converged to implicate the chromosome 15q25.1 region, which includes the CHRNA5-CHRNA3-CHRNB4 cholinergic nicotinic receptor subunit genes. In particular, association with the nonsynonymous CHRNA5 SNP rs16969968 and correlates has been replicated in several independent studies. Extensive genotyping of this region has suggested additional statistically distinct signals for nicotine dependence, tagged by rs578776 and rs588765. One goal of the Consortium for the Genetic Analysis of Smoking Phenotypes (CGASP is to elucidate the associations among these markers and dichotomous smoking quantity (heavy versus light smoking, lung cancer, and chronic obstructive pulmonary disease (COPD. We performed a meta-analysis across 34 datasets of European-ancestry subjects, including 38,617 smokers who were assessed for cigarettes-per-day, 7,700 lung cancer cases and 5,914 lung-cancer-free controls (all smokers, and 2,614 COPD cases and 3,568 COPD-free controls (all smokers. We demonstrate statistically independent associations of rs16969968 and rs588765 with smoking (mutually adjusted p-values<10(-35 and <10(-8 respectively. Because the risk alleles at these loci are negatively correlated, their association with smoking is stronger in the joint model than when each SNP is analyzed alone. Rs578776 also demonstrates association with smoking after adjustment for rs16969968 (p<10(-6. In models adjusting for cigarettes-per-day, we confirm the association between rs16969968 and lung cancer (p<10(-20 and observe a nominally significant association with COPD (p = 0.01; the other loci are not significantly associated with either lung cancer or COPD after adjusting for rs16969968. This study provides strong evidence that multiple statistically distinct loci in this region affect smoking behavior. This study is also the first report of association between rs588765

  1. The Process and Reason of the Change of Oil-Water Contact of Shahejie Formation in BZ25-1 Oilfield

    Science.gov (United States)

    Cong, F.; Liu, J.

    2015-12-01

    Due to the influence of Neo-tectonic movement, the Shahejie reservoirs in Bohai Bay Basin has undergone late-stage transformation and adjustment, causing the oil-water contact to change. Through studying the changing history of oil-water contact, we can better restore petroleum accumulation process and analyze oil distribution pattern. Based on reservoir geochemistry theory and drilling and logging data, grains with oil inclusion was analyzed, and oil-bearing property, organic extracts and biomarkers was used to determine the present and paleo-oil water contact of Shahejie formation in BZ25-1 oilfield. It suggested that the paleo and present oil-water contact in Shahejie formation locates in different depth, and that Shahejie formation has gone through three petroleum charging stages and has also undergone reservoir adjustment. The POWC(paleo-oil-water contact) of E2S2 reservoirs in BZ25-1-5 well and E2S2 reservoirs in BZ25-1-3 well is lower than OWC(present oil-water contact) at least for 9m and at most for 400m, but the POWC of E2S3 reservoirs in BZ25-1-5 well is higher than OWC at least for 20m and at most for 27.5m. The petroleum accumulation process and the reason for oil-water contact adjustment were studied based on burial history, petroleum generation history, fault re-activation rate and petroleum charging history. It suggested that the three petroleum charging stages are Mid-Miocene(11.5Ma), Late Miocene-Pliocene(6.5-3.5Ma) and Quaternary(2.5Ma-present), among which the second~third charging episode is seen as the major petroleum accumulation stage. The re-activeted faults in several different periods not only served as preferential path for petroleum vertical migration, but also caused petroleum leakage through faults. The petroleum leakage mainly occurred in Neo-tectonic movement period(after 3.5Ma), during which petroleum vertically leaked through re-activated faults and migrated to shallow reservoirs or spilled over surface, meanwhile due to constant

  2. Mucosal B Cells Are Associated with Delayed SIV Acquisition in Vaccinated Female but Not Male Rhesus Macaques Following SIVmac251 Rectal Challenge.

    Directory of Open Access Journals (Sweden)

    Iskra Tuero

    2015-08-01

    Full Text Available Many viral infections, including HIV, exhibit sex-based pathogenic differences. However, few studies have examined vaccine-related sex differences. We compared immunogenicity and protective efficacy of monomeric SIV gp120 with oligomeric SIV gp140 in a pre-clinical rhesus macaque study and explored a subsequent sex bias in vaccine outcome. Each immunization group (16 females, 8 males was primed twice mucosally with replication-competent Ad-recombinants encoding SIVsmH4env/rev, SIV239gag and SIV239nefΔ1-13 and boosted twice intramuscularly with SIVmac239 monomeric gp120 or oligomeric gp140 in MF59 adjuvant. Controls (7 females, 5 males received empty Ad and MF59. Up to 9 weekly intrarectal challenges with low-dose SIVmac251 were administered until macaques became infected. We assessed vaccine-induced binding, neutralizing, and non-neutralizing antibodies, Env-specific memory B cells and plasmablasts/plasma cells (PB/PC in bone marrow and rectal tissue, mucosal Env-specific antibodies, and Env-specific T-cells. Post-challenge, only one macaque (gp140-immunized remained uninfected. However, SIV acquisition was significantly delayed in vaccinated females but not males, correlated with Env-specific IgA in rectal secretions, rectal Env-specific memory B cells, and PC in rectal tissue. These results extend previous correlations of mucosal antibodies and memory B cells with protective efficacy. The gp140 regimen was more immunogenic, stimulating elevated gp140 and cyclic V2 binding antibodies, ADCC and ADCP activities, bone marrow Env-specific PB/PC, and rectal gp140-specific IgG. However, immunization with gp120, the form of envelope immunogen used in RV144, the only vaccine trial to show some efficacy, provided more significant acquisition delay. Further over 40 weeks of follow-up, no gp120 immunized macaques met euthanasia criteria in contrast to 7 gp140-immunized and 2 control animals. Although males had higher binding antibodies than females, ADCC

  3. The LLNL Heavy Element Facility -- Facility Management, Authorization Basis, and Readiness Assessment Lessons Learned in the Heavy Element Facility (B251) Transition from Category II Nuclear Facility to Radiological Facility

    Energy Technology Data Exchange (ETDEWEB)

    Mitchell, M; Anderson, B; Brown, E; Gray, L

    2006-04-10

    This paper presents Facility Management, Readiness Assessment, and Authorization Basis experience gained and lessons learned during the Heavy Element Facility Risk Reduction Program (RRP). The RRP was tasked with removing contaminated glove boxes, radioactive inventory, and contaminated ventilation systems from the Heavy Element Facility (B251) at Lawrence Livermore National Laboratory (LLNL). The RRP was successful in its goal in April 2005 with the successful downgrade of B251 from a Category II Nuclear Facility to a Radiological Facility. The expertise gained and the lessons learned during the planning and conduct of the RRP included development of unique approaches in work planning/work control (''Expect the unexpected and confirm the expected'') and facility management. These approaches minimized worker dose and resulted in significant safety improvements and operational efficiencies. These lessons learned can help similar operational and management activities at other sites, including facilities restarting operations or new facility startup. B251 was constructed at LLNL to provide research areas for conducting experiments in radiochemistry using transuranic elements. Activities at B251 once included the preparation of tracer sets associated with the underground testing of nuclear devices and basic research devoted to a better understanding of the chemical and nuclear behavior of the transuranic elements. Due to the age of the facility, even with preventative maintenance, facility safety and experimental systems were deteriorating. A variety of seismic standards were used in the facility design and construction, which encompassed eight building increments constructed over a period of 26 years. The cost to bring the facility into compliance with the current seismic and other requirements was prohibitive, and simply maintaining B251 as a Category II nuclear facility posed serious cost considerations under a changing regulatory environment

  4. The effects of leptin in combination with a cannabinoid receptor 1 antagonist, AM 251, or cannabidiol on food intake and body weight in rats fed a high-fat or a free-choice high sugar diet.

    Science.gov (United States)

    Wierucka-Rybak, M; Wolak, M; Bojanowska, E

    2014-08-01

    High intake of fats and sugars has prompted a rapid growth in the number of obese individuals worldwide. To further investigate whether simultaneous pharmacological intervention in the leptin and cannabinoid system might change food and water intake, preferences for palatable foods, and body weight, we have examined the effects of concomitant intraperitoneal administration of leptin and AM 251, a cannabinoid 1 (CB1) receptor antagonist, or cannabidiol (CBD), a plant cannabinoid, in rats maintained on either a high-fat (HF) diet (45% energy from fat) or free-choice (FC) diet consisting of high-sucrose and normal rat chow (83% and 61% energy from carbohydrates, respectively). Leptin at a dose of 100 μg/kg injected individually for 3 subsequent days to rats fed a HF diet reduced significantly the daily caloric intake and inhibited body weight gain. The hormone had no significant effects, however, on either caloric intake, body weight or food preferences in rats fed an FC diet. Co-injection of leptin and 1 mg/kg AM 251 resulted in a further significant decrease in HF diet intake and a profound reduction in body weight gain both in HF diet- and FC diet-fed rats. This drug combination, however, had no effect on the consumption of high-sucrose chow. In contrast, 3mg/kg of CBD co-injected with leptin did not modify leptin effects on food intake in rats maintained on an FC or HF diet. None of the drug combinations affected water consumption. It is concluded that the concomitant treatment with leptin and AM 251 attenuated markedly body weight gain in rats maintained on high-calorie diets rich in fat and carbohydrates but did not affect preferences for sweet food.

  5. Reaction of (chloro carbonyl) phenyl ketene with 5-amino pyrazolones: Synthesis, characterization and theoretical studies of 7-hydroxy-6-phenyl-3-(phenyldiazenyl)pyrazolo[1,5-a]pyrimidine-2,5(1H,4H)-dione derivatives

    Science.gov (United States)

    Zahedifar, Mahboobeh; Razavi, Razieh; Sheibani, Hassan

    2016-12-01

    New 7-hydroxy-6-phenyl-3-(phenyldiazenyl)pyrazolo[1,5-a]pyrimidine-2,5(1H,4H)-dione derivatives were synthesized from the reaction of (chlorocarbonyl)phenyl ketene and 5-amino pyrazolones in high to excellent yields and short reaction times. Structures of the new compounds were fully characterized by their spectral data IR, 1H NMR, and 13C NMR and by the theoretical results. Density Functional Theory (DFT) was used to optimize the structures, compute the energies and vibrational frequencies IR and 1H NMR shielding tensors of the desired products. The theoretical results excellent are compared with the experimental data.

  6. 社区康复训练对251例不同类型精神障碍患者康复效果比较研究%A Compared Study about the Effects of Community Rehabilitation Training for the Treatment of Patients with 251 Differ-ent Categories Mental Disorders

    Institute of Scientific and Technical Information of China (English)

    李合群; 李墨池; 崔拥军; 张加强

    2014-01-01

    Objective :To evaluate the effects of the rehabilitation training for treatment of patients among different mental disorders in community .Methods :Enrolled 251 patients in rehabilitation period with different mental disorders accepted rehabilitation training ,96 patients did not accept any rehabilitation training during the period of study as con-trol groups .Self Accept Questionnaire ,Social Disability Screening Scale ,Positive Affect and Negative Affect Scale and TDL Life Quality Scale were used to evaluated the effect of rehabilitation training .Results:(1)Almost all evaluated in-dictors have significant difference among four different mental disorders excluded the positive and affective balance fac-tor .(2)The patient with schizophrenia scored lower in most factors of SDSS than other mental disorders .(3)Compared with neurosis disorder and stress related disorder ,mood disorder scored lower and had statistically significance .(4) Compared with stress related disorder ,neurosis disorder scored significantly higher in positive accept ,self accept ,and some factors of TDL-life quality .Conclusion:The effects of rehabilitation training are influenced by many factors ,such as the categories of the mental disorder ,the course of the disorder and other factors .The effects of community rehabili-tation training are differently among different categories of disorders .The effect of community rehabilitation training in patients with Schizophrenia had scored lower significantly than other mental disorders .%目的:探讨社区康复训练方式对于不同类型精神障碍的应用效果。方法:根据自愿入组原则募集目前处于康复期的精神障碍患者251人入组接受社区康复训练,对照组96人,研究期间不接受康复训练。采用“自我接纳问卷(SAQ)”、“社会功能缺陷量表(SDSS)”、“情感量表”及“TDL生命质量量表”评估康复效果。结果:(1)在4组不同诊断间的结果比较中

  7. A t(3;9)(q25.1;q34.3) translocation leading to OLFM1 fusion transcripts in Gilles de la Tourette syndrome, OCD and ADHD

    DEFF Research Database (Denmark)

    Bertelsen, Birgitte; Melchior, Linea; Jensen, Lars Riff

    2015-01-01

    compulsive disorder, attention-deficit/hyperactivity-disorder, as well as other comorbidities, and a translocation t(3;9)(q25.1;q34.3) inherited from a mother with tics. Mate-pair sequencing revealed that the translocation breakpoints truncated the olfactomedin 1 (OLFM1) gene and two uncharacterized......Gilles de la Tourette syndrome (GTS) is a neuropsychiatric disorder with a strong genetic etiology; however, finding of candidate genes is hampered by its genetic heterogeneity and the influence of non-genetic factors on disease pathogenesis. We report a case of a male patient with GTS, obsessive...... transcripts. Reverse-transcription PCR identified several fusion transcripts in the carriers, and OLFM1 expression was found to be high in GTS-related human brain regions. As OLFM1 plays a role in neuronal development it is a likely candidate gene for neuropsychiatric disorders and haploinsufficiency of OLFM1...

  8. Developmental delay and facial dysmorphism in a child with an 8.9 Mb de novo interstitial deletion of 3q25.1-q25.32: Genotype-phenotype correlations of chromosome 3q25 deletion syndrome.

    Science.gov (United States)

    Moortgat, Stephanie; Verellen-Dumoulin, Christine; Maystadt, Isabelle; Parmentier, Benoit; Grisart, Bernard; Hennecker, Jean-Luc; Destree, Anne

    2011-01-01

    Interstitial deletions of the long arm of chromosome 3 are rare and detailed genotype-phenotype correlations are not well established. We report on the clinical, cytogenetic and molecular findings of a 5-year-old patient with a de novo interstitial deletion from 3q25.1 to 3q25.32. Clinical features include relative microcephaly, developmental delay and facial dysmorphism with a coarse face, ptosis, synophrys, epicanthic folds, broad nasal bridge, long philtrum, large mouth with full lips, dysplastic and low-set ears. Revealed by conventional banding techniques, the deleted region of 8.9 Mb was confirmed by fluorescent in situ hybridization (FISH) analyses and array comparative genomic hybridization (array-CGH). To our knowledge, this is the smallest interstitial deletion reported in the 3q25 region. The phenotype of our patient is compared with the 10 previously reported cases implicating the 3q25 region.

  9. Differences in time of virus appearance in the blood and virus-specific immune responses in intravenous and intrarectal primary SIVmac251 infection of rhesus macaques; a pilot study

    Directory of Open Access Journals (Sweden)

    Washington Parks Robyn

    2001-07-01

    Full Text Available Abstract Background HIV-I can be transmitted by intravenous inoculation of contaminated blood or blood product or sexually through mucosal surfaces. Here we performed a pilot study in the SIVmac251 macaque model to address whether the route of viral entry influences the kinetics of the appearance and the size of virus-specific immune in different tissue compartments. Methods For this purpose, of 2 genetically defined Mamu-A*01-positive macaques, 1 was exposed intravenously and the other intrarectally to the same SIVmac251 viral stock and virus-specific CD8+ T-cells were measured within the first 12 days of infection in the blood and at day 12 in several tissues following euthanasia. Results Virus-specific CD8+ T-cell responses to Gag, Env, and particularly Tat appeared earlier in the blood of the animal exposed by the mucosal route than in the animal exposed intravenously. The magnitude of these virus-specific responses was consistently higher in the systemic tissues and GALT of the macaque exposed by the intravenous route, suggesting a higher viral burden in the tissues as reflected by the faster appearance of virus in plasma. Differences in the ability of the virus-specific CD8+ T-cells to respond in vitro to specific peptide stimulation were also observed and the greatest proliferative ability was found in the GALT of the animal infected by the intrarectal route. Conclusions These data may suggest that the natural mucosal barrier may delay viral spreading. The consequences of this observation, if confirmed in studies with a larger number of animals, may have implications in vaccine development.

  10. Effect of Notch -1 gene uprugulation on AKT -mTOR signal of U251 cell%上调 Notch-1基因对 U215细胞 AKT -mTOR 通路的影响

    Institute of Scientific and Technical Information of China (English)

    楚可新; 戴明明; 林勤; 赵能江

    2016-01-01

    Objective:To investigate the effect of Notch -1 gene up -regulation on biological hehaviors of U215 cell and explore its effects on AKT -mTOR signal.Methods:U251 cells were infected with over -expression NICD or control lentivirus,the mRNA and protein expression of Notch -1 gene were examined with RT -PCR and Western -Blot,the cell proliferation was assayed with MTT,cell cycle was determined by flow cytometry,the effects of Notch -1 gene on U251 cell invasion were studied using matrigel coated Transwell chambers,the effects of Notch -1 up -gula-tion on AKT,mTOR,P70S6K and 4Ebp -1 protein expression were also analyzed.Results:Compared with the un-treated and empty group,after 72h of infection,the protein and mRNA expression of Notch -1 increased(P <0.05). It was found that up -regulation of Notch -1 promoted U251 cell growth,invasion and S -phase cells were increased (P <0.05).Up -regulation of Notch -1 promoted Cyclin D1,CDK -4 protein expression,and p -AKT and p -mTOR also increased in Notch -1 up -regulated U251 cells.Conclusion:Up -regulation Notch -1 promotes U251 glioma cell proliferation and invasion,the mechanism was partly through regulating AKT -mTOR signaling.%目的:观察上调 Notch -1基因对人胶质瘤 U251细胞生学物行为及 AKT -mTOR 信号通路的影响。方法:采用 pNL -NICD 慢病毒感染 U251细胞,以 RT -PCR 和 Western -Blot 检测 Notch -1基因 mRNA 和蛋白的表达,以 MTT 检测细胞增殖能力,流式细胞术检测细胞周期变化,Transwell 实验检测细胞侵袭能力,同时检测 Notch -1上调对 AKT、mTOR、P70S6K、4Ebp -1蛋白的影响。结果:与对照组比较,NICD 慢病毒转染72h后,Notch -1基因 mRNA 和蛋白表达明显增高(P <0.01);Notch -1上调明显促进 U251细胞增殖、侵袭、促进细胞进入 S 期,增加周期蛋白 Cyclin D1、CDK -4的表达,促进 AKT、mTOR 蛋白磷酸化。结论:上调 Notch -1基因促进 U251

  11. Tracking past changes in lake-water phosphorus with a 251-lake calibration dataset in British Columbia: tool development and application in a multiproxy assessment of eutrophication and recovery in Osoyoos Lake, a transboundary lake in western North America

    Directory of Open Access Journals (Sweden)

    Brian Fraser Cumming

    2015-08-01

    Full Text Available Recently there has been an active discussion about the potential and challenges of tracking past lake-water trophic state using paleolimnological methods. Herein, we present analyses of the relationship between modern-day diatom assemblages from the surface sediments of 251 fresh-water lakes from British Columbia and contemporary limnological variables. Total phosphorus (TP was significantly related to the modern distribution of diatom assemblages. The large size of this new calibration dataset resulted in higher abundances and occurrences of many diatom taxa thereby allowing a more accurate quantification of the optima of diatom taxa to TP in comparison to previous smaller calibration datasets. Robust diatom-based TP inference models with a moderate predictive power were developed using weighted-averaging regression and calibration. Information from the calibration dataset was used to interpret changes in the diatom assemblages from the north and south basins of Osoyoos Lake, in conjunction with fossil pigment analyses. Osoyoos Lake is a large salmon-bearing lake that straddles the British Columbia-Washington border and has undergone cultural eutrophication followed by recovery due to substantial mitigation efforts in managing sources of nutrients. Both diatom assemblages and sedimentary pigments indicate that eutrophication began c. 1950 in the north basin and c. 1960 in the southern basin, reaching peak levels of production between 1960 and 1990, after which decreases in sedimentary pigments occurred, as well as decreases in the relative abundance and concentrations of diatom taxa inferred to have high TP optima. Post-1990 changes in the diatom assemblage suggests conditions have become less productive with a shift to taxa more indicative of lower TP optima in concert with measurements of declining TP, two of these diatom taxa, Cyclotella comensis and Cyclotella gordonensis, that were previously rare are now abundant.

  12. 36 CFR 251.82 - Appealable decisions.

    Science.gov (United States)

    2010-07-01

    ... CFR 292.17 and 292.18. (6) Permits and agreements regarding mineral materials (petrified wood and...) under 36 CFR 228, subpart C. (7) Permits authorizing exercise of mineral rights reserved in...

  13. 15 CFR 904.251 - Evidence.

    Science.gov (United States)

    2010-01-01

    ... the agency as an expert body. Where a decision or part thereof rests on official notice of a material..., which include, but are not limited to, matters of a national security, business, personal, or... relevant material or source, whether or not submitted by a party. (2) Exhibits in a foreign language...

  14. 42 CFR 2.51 - Medical emergencies.

    Science.gov (United States)

    2010-10-01

    ... identifying information may be disclosed to medical personnel who have a need for information about a patient... information may be disclosed to medical personnel of the Food and Drug Administration (FDA) who assert a... forth in writing: (1) The name of the medical personnel to whom disclosure was made and...

  15. 36 CFR 251.53 - Authorities.

    Science.gov (United States)

    2010-07-01

    ... structures and facilities for recreation, public convenience, or safety; (ii) industrial or commercial..., flumes, laterals, pipes, pipelines, tunnels, and other facilities and systems for the impoundment... facilities in connection therewith; (3) Pipelines, slurry and emulsion systems, and conveyor belts...

  16. 40 CFR 60.251 - Definitions.

    Science.gov (United States)

    2010-07-01

    ... instrument that operates on triboelectric, light scattering, light transmittance, or other effect to... combustion concentration means the theoretical emissions (nanograms per joule (ng/J) or pounds per...

  17. 50 CFR 216.251 - Effective dates.

    Science.gov (United States)

    2010-10-01

    ..., DEPARTMENT OF COMMERCE MARINE MAMMALS REGULATIONS GOVERNING THE TAKING AND IMPORTING OF MARINE MAMMALS Taking Marine Mammals Incidental to Conducting Precision Strike Weapon Missions in the Gulf of Mexico §...

  18. 25 CFR 700.251 - Fees.

    Science.gov (United States)

    2010-04-01

    ..., and (4) Resolving legal and policy issues affecting access to requested records. (c) Waiver or... international organizations when to do so without charge is an appropriate courtesy, or when the recipient is... established business custom (e.g., furnishing personal reference data to prospective employers of...

  19. 36 CFR 251.51 - Definitions.

    Science.gov (United States)

    2010-07-01

    ..., pipeline, electronic transmission line, fence, water transmission facility, or fiber optic cable. Major... for pecuniary remuneration or other gain any saddle or pack animal, vehicle, boat, camping gear,...

  20. 16 CFR 251.1 - The guide.

    Science.gov (United States)

    2010-01-01

    ... supplier's offers. Prior to advertising a “Free” promotion, a supplier should offer the product as promoted... good faith, to discontinue the offer after a limited time and to commence selling the product or... selling the product or service promoted, separately, at the same price at which it was promoted with...

  1. 36 CFR 251.58 - Cost recovery.

    Science.gov (United States)

    2010-07-01

    ... applications and monitoring costs for special use authorizations. Applicants and holders shall submit sufficient information for the authorized officer to estimate the number of hours required to process their applications or monitor their authorizations. Cost recovery fees are separate from any fees charged for the...

  2. 30 CFR 206.251 - Definitions.

    Science.gov (United States)

    2010-07-01

    ..., water, or heavy media separation; drying; and related handling (or combination thereof). Contract means..., treatment with substances including chemicals or oils, and other preparation of the coal to the extent that..., production, preparation, and handling of lease products. Net-back method means a method for...

  3. 36 CFR 251.121 - Definitions.

    Science.gov (United States)

    2010-07-01

    .... Historical operator—a holder of a valid special use authorization to provide visitor services in a CSU under... section 102(6) of ANILCA (16 U.S.C. 3197). Preferred operator—a Native Corporation that is determined... as providing food, accommodations, transportation, tours, and outfitting and guiding, except...

  4. 37 CFR 251.13 - Closed meetings.

    Science.gov (United States)

    2010-07-01

    ... be kept secret by Executive Order, in the interests of national defense or foreign policy; or (b) If...) Interfere with enforcement proceedings; or (2) Deprive a person of the right to a fair trial or impartial... confidential source or, in the case of a criminal investigation or a national security...

  5. 37 CFR 251.60 - Scope.

    Science.gov (United States)

    2010-07-01

    ... affecting cable (17 U.S.C. 111), the making of ephemeral recordings (17 U.S.C. 112), certain digital audio transmissions (17 U.S.C. 114), the manufacture and distribution of phonorecords, including digital phonorecord...), noncommercial educational broadcasting (17 U.S.C. 118) and satellite carriers (17 U.S.C. 119). Those...

  6. 36 CFR 251.35 - Petersburg watershed.

    Science.gov (United States)

    2010-07-01

    ... Federal employees, holders of Forest Service contracts, or Forest Service agents; (2) The operation, maintenance, and improvement of the municipal water system by Federal and State officials and employees of the... Ranger. (d) Unauthorized entrance upon lands within the watershed is subject to punishment as provided...

  7. 37 CFR 251.39 - Remedies.

    Science.gov (United States)

    2010-07-01

    ....39 Patents, Trademarks, and Copyrights COPYRIGHT OFFICE, LIBRARY OF CONGRESS COPYRIGHT ARBITRATION ROYALTY PANEL RULES AND PROCEDURES COPYRIGHT ARBITRATION ROYALTY PANEL RULES OF PROCEDURE Standards of... who engaged in the ethical violation— (1) Referral of the matter to the bar or...

  8. 30 CFR 251.1 - Definitions.

    Science.gov (United States)

    2010-07-01

    ... occurrence means the direct or indirect detection during drilling operations of any liquid or gaseous... samples that have not been analyzed, processed, or interpreted. Deep stratigraphic test means drilling... drilling, whether on or off a geological structure. Geological and geophysical scientific research...

  9. 信用供给与中小企业融资行为研究--基于温州等三地251个中小企业的实证研究

    Institute of Scientific and Technical Information of China (English)

    石涛

    2014-01-01

    以温州等三地251个中小企业为样本,运用PROBIT模型,深入分析在国家金融政策偏紧的情况下,信用供给与中小企业融资行为的关系。发现年龄、发展困境与抵押品提供之间呈现负向关系,教育程度、抵押品类型、加速贷款的原因、同一年机构贷款的次数与抵押品提供之间呈现正向关系;企业申贷往金融机构跑的次数、贷款路径的选择对参与评级行为产生负向影响,教育程度、同一机构年贷款次数、选择参加此类机构的原因、是否考虑信用对参与评级行为产生正向影响;年龄、流动资产、抵押品类型、选择此类机构的原因、是否参加评级对融资路径的选择产生负向影响,销售总额、加速贷款的原因、是否参加社会金融组织、是否选择抵押品对融资机构路径的选择产生正向影响。为此,必须创新金融服务,提高中小企业融资效率。%The credit supply is an important factor which affects the behavior of SME. Under the back-ground of fiscal austerity, and based on the sample from the city of Wenzhou et al, by using the method of PROBIT, this paper analysis the relationship between the supply of credit and the financing behavior of SME. We found that:the relationship between age, development difficulties and collateral provide is negative;educational level, collateral types, the cause which accelerated loan, the time of loan from the same institution in one year and collateral provide is positive. The relationship between the time of loan application from finance institution, the path of loan choosing and the action of rating is negative;the re-lationship between the educational level, the time of loans in the same institution, the cause of choose institution, whether to consider participation in the credit rating and the action of rating is positive. The relationship between age, current assets, collateral type, the cause of choose

  10. 1-(2-炔丙基)-7,8-二氢喹啉-2,5(1H,6H)-二酮的合成%Synthesis of 1-(Prop-2-yn-1-yl)-7,8-dihydroquinoline-2,5 (1H, 6H)-dione

    Institute of Scientific and Technical Information of China (English)

    刘海波; 许明; 丁俊杰

    2013-01-01

    以1,3-环己二酮为原料,依次经亚胺化反应、Michael加成-环化反应、烷基化反应合成了含有端基炔的喹啉酮衍生物——1-(2-炔丙基)-7,8-二氢喹啉-2,5(1H,6H)-二酮,其结构经1H NMR,HR-MS和2D NMR确证.%1 -( Prop-2-yn-1-yl) -7,8-dihydroquinoline-2,5 (1H, 6H) -dione ( 4) was simply prepared from 1,3-cyclohexadione by a three-step reaction of imidization, Michael reaction-intermolecular cy-clization and alkylation. Two configuration stereo-isomers of 4 were isolated by chemical technology. The structures were confirmed by 1H NMR, HR-MS and 2D NMR.

  11. Effect of methylation levels of GDNF gene promoterⅠregion on its transcription in glioma U251 cells%胶质瘤细胞GDNF基因启动子Ⅰ区甲基化水平对其基因转录的影响

    Institute of Scientific and Technical Information of China (English)

    续继军; 孟铸; 李志鹏; 陈欣; 任庆先; 秦汉

    2015-01-01

    目的:探讨胶质瘤细胞胶质细胞原性神经营养因子(GDNF)基因启动子Ⅰ区甲基化水平对其基因转录的影响。方法体外培养胶质瘤U251细胞,加入不同浓度5-氮杂胞苷(浓度分别为1、5、10和20μmol/L)干预,以加入PBS为对照。采用重亚硫酸盐测序法测定GDNF基因启动子Ⅰ区甲基化水平,RT-PCR检测GDNF mRNA的表达。结果与PBS组相比,1μmol/L 5-氮杂胞苷对GDNF基因启动子Ⅰ区甲基化水平无显著影响(P>0.05),5、10和20μmol/L 5-氮杂胞苷均显著降低其甲基化水平(P0.05),5μmol/L 5-氮杂胞苷显著增加其表达水平(P0.05). Conclusion The demethylation of GDNF gene promoterⅠregion, which is produced by 5-aza-CR, may influence the expression of GDNF in U251 cell.

  12. 37 CFR 251.35 - Outside employment and other activities.

    Science.gov (United States)

    2010-07-01

    ... arbitrator shall— (1) Engage in any outside business or other activity that would cause a reasonable person to question the arbitrator's ability to render an impartial decision; (2) Accept any speaking... the arbitrator's knowledge and acquiescence to the arbitrator's parent, sibling, spouse, child,...

  13. 37 CFR 251.37 - Use of nonpublic information.

    Science.gov (United States)

    2010-07-01

    ... arbitrator shall disclose in any manner any information contained in filings, pleadings, or evidence that the arbitration panel has ruled to be confidential in nature. (b) Unless required by law, no arbitrator shall...) The CARP report before its submission to the Librarian of Congress. (c) No arbitrator shall engage...

  14. 37 CFR 251.48 - Rules of evidence.

    Science.gov (United States)

    2010-07-01

    ... furnished: (1) Sample surveys. (i) A clear description of the survey design, the definition of the universe...) Studies involving statistical methodology. (i) The formula used for statistical estimates; (ii)...

  15. Dicty_cDB: SHC251 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available 98 |DT364098.1 JGI_ANNN9278.rev ANNN Pimephales promelas Whole (L) Pimephales promelas... cDNA clone ANNN9278 3', mRNA sequence. 48 0.24 1 DT347978 |DT347978.1 JGI_CAAX10802.fwd CAAX Pimephales promelas... testis 7-8 month adults, males and females pooled (L) Pimephales promelas cDNA clone CAAX10802 5'..., mRNA sequence. 48 0.24 1 DT309975 |DT309975.1 JGI_CAAX1517.fwd CAAX Pimephales promelas testis 7-8 month a...dults, males and females pooled (L) Pimephales promelas cDNA clone CAAX1517 5', m

  16. 76 FR 251 - Country of Origin Labeling of Packed Honey

    Science.gov (United States)

    2011-01-04

    ... aid, or licensed sampler. Pursuant to the amendment of the Act by the 2008 Farm Bill, section 52.54... Subjects in 7 CFR Part 52 Food grades and standards, Food labeling, Honey, Miscellaneous products... inspector, inspector's aid, or licensed sampler. Any interference with, obstruction of, or...

  17. 40 CFR 264.251 - Design and operating requirements.

    Science.gov (United States)

    2010-07-01

    ... attenuative capacity and thickness of the liners and soils present between the pile and ground water or... portion of a waste pile) must have: (1) A liner that is designed, constructed, and installed to prevent any migration of wastes out of the pile into the adjacent subsurface soil or ground water or...

  18. 37 CFR 251.33 - Ex parte communications.

    Science.gov (United States)

    2010-07-01

    ... appears on a current arbitrator list. (d) Library and Copyright Office personnel. No person outside the... does not apply to procedural inquiries such as scheduling, filing requirements, status requests,...

  19. 36 CFR 251.9 - Management of Municipal Watersheds.

    Science.gov (United States)

    2010-07-01

    ... multiple use prescriptions in forest plans (36 CFR part 219). When a municipality desires protective... authorizations, the municipality must apply to the Forest Service for consideration of these needs. (b) When... incorporated in the forest plan without written agreements. Written agreements with municipalities to...

  20. 7 CFR 764.251 - Operating loan uses.

    Science.gov (United States)

    2010-01-01

    ... reorganizing a farm to improve its profitability; (2) Purchase of livestock, including poultry, farm equipment... non-compliance with the standards will cause substantial economic injury; (9) Borrower training costs... improve the farm's profitability provided the applicant has refinanced direct or guaranteed OL loans...

  1. 40 CFR 96.251 - Establishment of accounts.

    Science.gov (United States)

    2010-07-01

    ... transfers. (5) Delegation by CAIR authorized account representative and alternate CAIR authorized account... (CONTINUED) NOX BUDGET TRADING PROGRAM AND CAIR NOX AND SO2 TRADING PROGRAMS FOR STATE IMPLEMENTATION PLANS... purpose of holding and transferring CAIR SO2 allowances. An application for a general account...

  2. 29 CFR 1926.251 - Rigging equipment for material handling.

    Science.gov (United States)

    2010-07-01

    ... and to form eyes. The thread shall be in an even pattern and contain a sufficient number of stitches.... These grades are not recommended for overhead lifting and therefore are not covered by this code....

  3. 48 CFR 952.251-70 - Contractor employee travel discounts.

    Science.gov (United States)

    2010-10-01

    ... Federal Contractor employee travelers by AMTRAK, hotels, motels, or car rental companies, when use of such... employees. (d) Hotels/motels. Many lodging providers extend their discount rates for Federal employees...

  4. 36 CFR 251.80 - Purpose and scope.

    Science.gov (United States)

    2010-07-01

    ... scope. (a) This subpart provides a process by which those who hold or, in certain instances, those who apply for written authorizations to occupy and use National Forest System lands, may appeal a written...) The rules in this subpart seek to offer appellants a fair and deliberate process for appealing...

  5. 7 CFR 251.4 - Availability of commodities.

    Science.gov (United States)

    2010-01-01

    ... cost or waste, nutrition assistance to individuals in low-income groups. Such transfers will be... distribution of food to low-income households, including food from sources other than the Department. (4) State... agency determines that a claim exists against an eligible recipient agency, warehouseman, carrier or...

  6. Dicty_cDB: SFB251 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available GMGGGHXXRRXGXDXXHXX XXXXXXXXXXXX--- ---VRRSVQFQVEKGTRDGERIMLQGQGSEYPGVPPGDVIITIREKPKCKFQEKW**fnl hqtfkiirlnrw*sihykhigs...x xxxxxxxxxxxx--- ---VRRSVQFQVEKGTRDGERIMLQGQGSEYPGVPPGDVIITIREKPKCKFQEKW**fnl hqtfkiirlnrw*sihykhigs

  7. 37 CFR 251.61 - Commencement of adjustment proceedings.

    Science.gov (United States)

    2010-07-01

    ... respect to the carriage by cable systems of broadcast signals, or with respect to syndicated and sports... July 1, 2001 and at 5-year intervals thereafter, or (ii) During a 60-day period prescribed by...

  8. Dicty_cDB: AFO251 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available ) Pyrocystis lunula actin mRNA, comp... 252 3e-66 AY221950_1( AY221950 |pid:none) Pseudo-nitzschia multise...ries actin... 252 3e-66 protein update 2009. 4.26 PSORT psg: 0.84 gvh: 0.59 alm: 0.

  9. 1935 15' Quad #251 Aerial Photo Mosaic Index

    Data.gov (United States)

    Earth Data Analysis Center, University of New Mexico — Aerial Photo Reference Mosaics contain aerial photographs that are retrievable on a frame by frame basis. The inventory contains imagery from various sources that...

  10. 36 CFR 251.56 - Terms and conditions.

    Science.gov (United States)

    2010-07-01

    ... the special use authorization as determined by a risk assessment, prepared in accordance with... and otherwise protect the environment; (C) Require compliance with applicable air and water quality... location, design and plans (or standards, if appropriate) of all developments within the authorized...

  11. Dicty_cDB: CHQ251 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available |pid:none) Homo sapiens sucrase-isomaltase (a... 83 3e-15 BC116452_1( BC116452 |pid:none) Homo sapiens sucrase-isomalt...ase (a... 83 3e-15 ( P14410 ) RecName: Full=Sucrase-isomaltase, intestinal; Contains:... 83 3e-15 ...M22616_1( M22616 |pid:none) Human sucrase-isomaltase mRNA, partial... 83 3e-15 A3...) Neurospora crassa DNA linkage gro... 78 9e-14 L25926_1( L25926 |pid:none) Rat sucrase-isomaltase (SI) mRNA..., comp... 77 1e-13 ( P23739 ) RecName: Full=Sucrase-isomaltase, intestinal; Conta

  12. Dicty_cDB: CHS251 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available ibrary D Homo sapiens genomic clone Plate=3113 Col=14 Row=F, genomic survey sequence. 44 0.055 2 DT661445 |DT661445.1 est_e_glacial...is75 RWS Eubalaena glacialis cDNA, mRNA sequence. 46 0.71 1 AC116986 |AC116986.2 Dict

  13. Isospin dependence of reactions $^{48}$Ca+$^{243-251}$Bk

    CERN Document Server

    Shen, Caiwan; Boilley, Davoid; Kosenko, Grigory; Zhao, Enguang

    2008-01-01

    The fusion process of $^{48}$Ca induced reactions is studied with the two-step model. In this model, the fusion process is devided into two stages: first, the sticking stage where projectile and target come to the touching point over the Coulomb barrier from infinite distance, and second, the formation stage where the di-nucleus formed with projectile and target evolve to form the spherical compound nucleus from the touching point. By the use of the statistical evaporation model, the residue cross sections for different neutron evaporation channels are analyzed. From the results, optimum reactions are given to synthesize $Z$ = 117 element with $^{48}$Ca induced reactions.

  14. Time Warp Operating System, Version 2.5.1

    Science.gov (United States)

    Bellenot, Steven F.; Gieselman, John S.; Hawley, Lawrence R.; Peterson, Judy; Presley, Matthew T.; Reiher, Peter L.; Springer, Paul L.; Tupman, John R.; Wedel, John J., Jr.; Wieland, Frederick P.; Younger, Herbert C.

    1993-01-01

    Time Warp Operating System, TWOS, is special purpose computer program designed to support parallel simulation of discrete events. Complete implementation of Time Warp software mechanism, which implements distributed protocol for virtual synchronization based on rollback of processes and annihilation of messages. Supports simulations and other computations in which both virtual time and dynamic load balancing used. Program utilizes underlying resources of operating system. Written in C programming language.

  15. AF-Geospace User’s Manual Version 2.5.1 and Version 2.51P

    Science.gov (United States)

    2012-08-01

    169 The RAYTRACE -APP Module...scintillation code [WBMOD], a simplified HF ionospheric ray tracing module [ RAYTRACE -APP], and applications built on the NASA AE-8 and AP-8 radiation belt...stored in. Ext: The Ext option must be selected if electron densities are generated in dynamic mode and if RAYTRACE -APP is to be used to trace a

  16. (E-1-Phenyl-2-({5-[(1E-(2-phenylhydrazin-1-ylidenemethyl]-2-thienyl}methylidenehydrazine

    Directory of Open Access Journals (Sweden)

    James L. Wardell

    2010-03-01

    Full Text Available The title molecule, C18H16N4S, adopts a U-shape with the aromatic groups lying syn and orientated in the same direction as the thiophene S atom. The conformation about each of the C=N bonds is E. Overall, the molecule is curved as seen in the dihedral angle of 30.26 (19° formed between the terminal benzene rings. In the crystal, supramolecular chains along the c axis are formed by a combination of N—H...N hydrogen bonds and N—H...π interactions.

  17. 7 CFR 1710.251 - Construction work plans-distribution borrowers.

    Science.gov (United States)

    2010-01-01

    ...) Transmission and substation facilities required to support the distribution system; (6) Distribution equipment... 7 Agriculture 11 2010-01-01 2010-01-01 false Construction work plans-distribution borrowers. 1710...—distribution borrowers. (a) All distribution borrowers must maintain a current CWP approved by their board...

  18. Rational design of cyclodextrin glycosyltransferase from Bacillus circulans strain 251 to increase alpha-cyclodextrin production

    NARCIS (Netherlands)

    Uitdehaag, JCM; Penninga, D; van Alebeek, GJWM; Smith, LM; Dijkstra, BW; Dijkhuizen, L

    2000-01-01

    Cyclodextrin glycosyltransferases (CGTase) (EC;2.4.1.19) are extracellular bacterial enzymes that generate cyclodextrins from starch. All known CGTases produce mixtures of alpha, beta, and gamma-cyclodextrins. A maltononaose inhibitor bound to the active site of the CGTase from Bacillus circulans st

  19. 48 CFR 52.251-2 - Interagency Fleet Management System Vehicles and Related Services.

    Science.gov (United States)

    2010-10-01

    ... vehicles and the use of related services by the Contractor shall be in accordance with 41 CFR 101-39 and 41 CFR 101-38.301-1. (End of clause) ... 48 Federal Acquisition Regulations System 2 2010-10-01 2010-10-01 false Interagency...

  20. Dynamics of viral replication in blood and lymphoid tissues during SIVmac251 infection of macaques

    Directory of Open Access Journals (Sweden)

    Mannioui Abdelkrim

    2009-01-01

    Full Text Available Abstract Background Extensive studies of primary infection are crucial to our understanding of the course of HIV disease. In SIV-infected macaques, a model closely mimicking HIV pathogenesis, we used a combination of three markers -- viral RNA, 2LTR circles and viral DNA -- to evaluate viral replication and dissemination simultaneously in blood, secondary lymphoid tissues, and the gut during primary and chronic infections. Subsequent viral compartmentalization in the main target cells of the virus in peripheral blood during the chronic phase of infection was evaluated by cell sorting and viral quantification with the three markers studied. Results The evolutions of viral RNA, 2LTR circles and DNA levels were correlated in a given tissue during primary and early chronic infection. The decrease in plasma viral load principally reflects a large decrease in viral replication in gut-associated lymphoid tissue (GALT, with viral RNA and DNA levels remaining stable in the spleen and peripheral lymph nodes. Later, during chronic infection, a progressive depletion of central memory CD4+ T cells from the peripheral blood was observed, accompanied by high levels of viral replication in the cells of this subtype. The virus was also found to replicate at this point in the infection in naive CD4+ T cells. Viral RNA was frequently detected in monocytes, but no SIV replication appeared to occur in these cells, as no viral DNA or 2LTR circles were detected. Conclusion We demonstrated the persistence of viral replication and dissemination, mostly in secondary lymphoid tissues, during primary and early chronic infection. During chronic infection, the central memory CD4+ T cells were the major site of viral replication in peripheral blood, but viral replication also occurred in naive CD4+ T cells. The role of monocytes seemed to be limited to carrying the virus as a cargo because there was an observed lack of replication in these cells. These data may have important implications for the targeting of HIV treatment to these diverse compartments.

  1. Operation REDWING. Project 2.51, Neutron-Flux Measurements. Extracted Version

    Science.gov (United States)

    1981-05-15

    The attenuation of the thermal -neutron flux is increased by adding borax. The neutron dose was reduced by a factor of approximately four by a...the thermal -neutron flux is increased by adding borax. The neutron dose was reduced by a factor of approximately four by a concrete box three feet on a...the ,, eutrons and their spatial distribution is of basic importance to the assessment of the effects of the neutrons from a device. Measurements of this

  2. 36 CFR 251.54 - Proposal and application requirements and procedures.

    Science.gov (United States)

    2010-07-01

    ... clearances, permits, and licenses; (vi) Environmental and management considerations; (vii) Special conditions... require environmental analysis and documentation. (f) Special requirements for certain proposals—(1) Oil and gas pipeline rights-of-way. These proposals must include the citizenship of the proponent(s)...

  3. 5 CFR 251.202 - Agency support to organizations representing Federal employees and other organizations.

    Science.gov (United States)

    2010-01-01

    ... determines that such action would benefit the agency's programs or would be warranted as a service to... or symposia or published in journals; (2) Using the authority under 5 U.S.C. 4109 and 4110, as... or activities and the agency can derive benefits from employee attendance at such meetings; and...

  4. Actinide Production in the Reaction of Heavy Ions withCurium-248

    Energy Technology Data Exchange (ETDEWEB)

    Moody, K.J.

    1983-07-01

    Chemical experiments were performed to examine the usefulness of heavy ion transfer reactions in producing new, neutron-rich actinide nuclides. A general quasi-elastic to deep-inelastic mechanism is proposed, and the utility of this method as opposed to other methods (e.g. complete fusion) is discussed. The relative merits of various techniques of actinide target synthesis are discussed. A description is given of a target system designed to remove the large amounts of heat generated by the passage of a heavy ion beam through matter, thereby maximizing the beam intensity which can be safely used in an experiment. Also described is a general separation scheme for the actinide elements from protactinium (Z = 91) to mendelevium (Z = 101), and fast specific procedures for plutonium, americium and berkelium. The cross sections for the production of several nuclides from the bombardment of {sup 248}Cm with {sup 18}O, {sup 86}Kr and {sup 136}Xe projectiles at several energies near and below the Coulomb barrier were determined. The results are compared with yields from {sup 48}Ca and {sup 238}U bombardments of {sup 248}Cm. Simple extrapolation of the product yields into unknown regions of charge and mass indicates that the use of heavy ion transfer reactions to produce new, neutron-rich above-target species is limited. The substantial production of neutron-rich below-target species, however, indicates that with very heavy ions like {sup 136}Xe and {sup 238}U the new species {sup 248}Am, {sup 249}Am and {sup 247}Pu should be produced with large cross sections from a {sup 248}Cm target. A preliminary, unsuccessful attempt to isolate {sup 247}Pu is outlined. The failure is probably due to the half life of the decay, which is calculated to be less than 3 minutes. The absolute gamma ray intensities from {sup 251}Bk decay, necessary for calculating the {sup 251}Bk cross section, are also determined.

  5. 47 CFR 51.321 - Methods of obtaining interconnection and access to unbundled elements under section 251 of the Act.

    Science.gov (United States)

    2010-10-01

    ... 47 Telecommunication 3 2010-10-01 2010-10-01 false Methods of obtaining interconnection and access... COMMUNICATIONS COMMISSION (CONTINUED) COMMON CARRIER SERVICES (CONTINUED) INTERCONNECTION Additional Obligations of Incumbent Local Exchange Carriers § 51.321 Methods of obtaining interconnection and access...

  6. Susceptibility to Ulcerative Colitis and Genetic Polymorphisms of A251G SOD1 and C-262T CAT

    OpenAIRE

    El-Kheshen Gadier; Moeini Maryam; Saadat Mostafa

    2016-01-01

    Background: Reactive oxygen species can attack and damage almost every molecule found in living cells, including proteins, carbohydrates, lipids, and DNA. For this reason, their production is normally tightly controlled. Among the most important defenses against these radicals are the super oxide dismutase (SOD) enzymes and catalase (CAT). In creasing attention has been given to the role of reactive oxygen metabolites in the pathogenesis of ulcerative colitis (UC), which is defined as an idio...

  7. The three transglycosylation reactions catalyzed by cyclodextrin glycosyltransferase from Bacillus circulans (strain 251) proceed via different kinetic mechanisms

    NARCIS (Netherlands)

    Veen, Bart A. van der; Alebeek, Gert-Jan W.M. van; Uitdehaag, Joost C.M.; Dijkstra, Bauke W.; Dijkhuizen, Lubbert

    2000-01-01

    Cyclodextrin glycosyltransferase (CGTase) catalyzes three transglycosylation reactions via a double displacement mechanism involving a covalent enzyme-intermediate complex (substituted-enzyme intermediate). Characterization of the three transglycosylation reactions, however, revealed that they diffe

  8. 46 CFR 251.1 - Applications for construction-differential subsidy under Title V, Merchant Marine Act, 1936, as...

    Science.gov (United States)

    2010-10-01

    ... standard design and the custom design of the owner's preference. Construction-differential subsidy will be.... Value engineering items considered mandatory by the Board prior to or during the development of the... percent (0.36×3) or 2.38 percent times the cost of the each of four ship bid price. This limitation...

  9. 76 FR 16038 - Proposed Collection; Comment Request for Prepaid Card Marketing Customer Survey, CS-10-251.

    Science.gov (United States)

    2011-03-22

    ..., IRS is hoping to leverage the theory and principles of social marketing. Social marketing principles and practices apply marketing principles to social programs. This data will provide the IRS with... Internal Revenue Service Proposed Collection; Comment Request for Prepaid Card Marketing Customer...

  10. A Vaccine against CCR5 Protects a Subset of Macaques upon Intravaginal Challenge with Simian Immunodeficiency Virus SIVmac251

    OpenAIRE

    2014-01-01

    As an alternative to targeting human immunodeficiency virus (HIV), we have developed vaccines targeting CCR5, a self-protein critically involved in HIV replication and pathogenesis. By displaying peptides derived from CCR5 at high density on the surface of virus-like particles, we can efficiently induce high-titer IgG antibodies against this self-molecule. Here, we investigated whether prophylactic immunization of rhesus macaques with a particle-based vaccine targeting two regions of macaque ...

  11. 36 CFR 251.14 - Conditions, rules and regulations to govern exercise of timber rights reserved in conveyance to...

    Science.gov (United States)

    2010-07-01

    ... locality for sales of similar National Forest timber and timber products. (4) Slash and debris resulting... facilities shall be of sufficient size and design to provide unobstructed flow of water. Equipment will not... perform currently as weather and soil conditions permit, the following erosion control work on portions...

  12. Silver nanoparticles outperform gold nanoparticles in radiosensitizing U251 cells in vitro and in an intracranial mouse model of glioma

    Science.gov (United States)

    Liu, Peidang; Jin, Haizhen; Guo, Zhirui; Ma, Jun; Zhao, Jing; Li, Dongdong; Wu, Hao; Gu, Ning

    2016-01-01

    Radiotherapy performs an important function in the treatment of cancer, but resistance of tumor cells to radiation still remains a serious concern. More research on more effective radiosensitizers is urgently needed to overcome such resistance and thereby improve the treatment outcome. The goal of this study was to evaluate and compare the radiosensitizing efficacies of gold nanoparticles (AuNPs) and silver nanoparticles (AgNPs) on glioma at clinically relevant megavoltage energies. Both AuNPs and AgNPs potentiated the in vitro and in vivo antiglioma effects of radiation. AgNPs showed more powerful radiosensitizing ability than AuNPs at the same mass and molar concentrations, leading to a higher rate of apoptotic cell death. Furthermore, the combination of AgNPs with radiation significantly increased the levels of autophagy as compared with AuNPs plus radiation. These findings suggest the potential application of AgNPs as a highly effective nano-radiosensitizer for the treatment of glioma. PMID:27757033

  13. Association of a single nucleotide polymorphism at 6q25.1, rs2046210 with breast cancer risk among Vietnamese population

    Directory of Open Access Journals (Sweden)

    Lan Thi Tuyet Nguyen

    2016-11-01

    Full Text Available Introduction: This study aims to confirm the association between SNP rs2046210 and breast cancer in the Vietnamese population. Methods: A case/control study has been performed with the sample size of 300 cases and 325 controls. High Resolution Melting method is optimised for SNP genotyping. Statistic logistic regression analysis was used along with dominant and recessive analysis to analyse the association between alleles and genotypes of the selected SNP and breast cancer risk. Results: With the selection population and the optimal HRM method, genotyping resulted in precise data for association analysis as the HWE test showed the distribution of genotypes in the population is in equilibrium (p > 0.05. The logistic regression association analysis showed SNP rs2046210 strongly associated with breast cancer risk in both allelic (p = 0.0015 and genotypic (p = 0.0064 analysis. The risk allele A, like other previous studies, strongly associated with increasing the risk of breast cancer up to 1.425 folds (OR [95%CI] = 1.425 [1.143- 1.777], p = 0.0015. However, it has shown the recessive effect in the additive analysis. Genotype AA showed stronger effect on the risk of breast cancer while the heterozygous AG genotypes showed weaker effect. Due to the small sample size, statistical power of this study is not high as expected (58.67%. But this case/control study confirms the association between SNP rs2046210 and the risk of breast cancer in the Vietnamese population. Conclusion: SNP rs2046210 is associated with breast cancer risk in the Vietnamese population. The risk allele A plays a role in increasing the risk of breast cancer in Vietnamese women.

  14. The role of arginine 47 in the cyclization and coupling reactions of cyclodextrin glycosyltransferase from Bacillus circulans strain 251 - Implications for product inhibition and product specificity

    NARCIS (Netherlands)

    van der Veen, Bart A.; Uitdehaag, J C M; Dijkstra, B W; Dijkhuizen, L

    2000-01-01

    Cyclodextrin glycosyltransferase (CGTase) (EC 2.4.1.19) is used for the industrial production of cyclodextrins. Its application, however, is hampered by the limited cyclodextrin product specificity and the strong inhibitory effect of cyclodextrins on CGTase activity. Recent structural studies have i

  15. The Streambank Erosion Control Evaluation and Demonstration Act of 1974, Section 32, Public Law 93-251. Appendix G. Demonstration Projects on Other Streams, Nationwide. Volume 2.

    Science.gov (United States)

    1981-12-01

    Clermont County residents in the labor market are employed in Hamilton County. The employment structure of Clermont County in 1970 consisted of a high...percent of manufacturing (42 percent), wholesale and retail (17 percent), and 11 percent in services. The remaining market consists of a relatively...listed by either the state or the U.S. Department of Interior, but which is capable of generating special enthusiasm and concern among birdwatchers , are

  16. SU-E-J-251: Incorporation of Pre-Therapy 18F-FDG Uptake with CT Texture Features in a Predictive Model for Radiation Pneumonitis Development

    Energy Technology Data Exchange (ETDEWEB)

    Anthony, G; Cunliffe, A; Armato, S; Al-Hallaq, H [The University of Chicago, Chicago, IL (United States); Castillo, R [Univ Texas Medical Branch of Galveston, Pearland, TX (United States); Pham, N [Baylor College of Medicine, Houston, TX (United States); Guerrero, T [Beaumont Health System, Royal Oak, MI (United States)

    2015-06-15

    Purpose: To determine whether the addition of standardized uptake value (SUV) statistical variables to CT lung texture features can improve a predictive model of radiation pneumonitis (RP) development in patients undergoing radiation therapy. Methods: Anonymized data from 96 esophageal cancer patients (18 RP-positive cases of Grade ≥ 2) were retrospectively collected including pre-therapy PET/CT scans, pre-/posttherapy diagnostic CT scans and RP status. Twenty texture features (firstorder, fractal, Laws’ filter and gray-level co-occurrence matrix) were calculated from diagnostic CT scans and compared in anatomically matched regions of the lung. The mean, maximum, standard deviation, and 50th–95th percentiles of the SUV values for all lung voxels in the corresponding PET scans were acquired. For each texture feature, a logistic regression-based classifier consisting of (1) the average change in that texture feature value between the pre- and post-therapy CT scans and (2) the pre-therapy SUV standard deviation (SUV{sub SD}) was created. The RP-classification performance of each logistic regression model was compared to the performance of its texture feature alone by computing areas under the receiver operating characteristic curves (AUCs). T-tests were performed to determine whether the mean AUC across texture features changed significantly when SUV{sub SD} was added to the classifier. Results: The AUC for single-texturefeature classifiers ranged from 0.58–0.81 in high-dose (≥ 30 Gy) regions of the lungs and from 0.53–0.71 in low-dose (< 10 Gy) regions. Adding SUVSD in a logistic regression model using a 50/50 data partition for training and testing significantly increased the mean AUC by 0.08, 0.06 and 0.04 in the low-, medium- and high-dose regions, respectively. Conclusion: Addition of SUVSD from a pre-therapy PET scan to a single CT-based texture feature improves RP-classification performance on average. These findings demonstrate the potential for more accurate prediction of RP using information from multiple imaging modalities. Supported, in part, by the National Institute of Biomedical Imaging and Bioengineering of the National Institutes of Health under grant number T32 EB002103; SGA receives royalties and licensing fees through the University of Chicago for computer-aided diagnosis technology. HA receives royalties through the University of Chicago for computer-aided diagnosis technology.

  17. The Streambank Erosion Control Evaluation and Demonstration Act of 1974, Section 32, Public Law 93-251. Appendix A. Literature Survey.

    Science.gov (United States)

    1981-12-01

    Dyer, C. E., "Revetment--Stabilization of the Channel of the Rio Grande from Caballo Dam to Fort Quitman, New Mexico-Texas," Symposium on Channel...Stabilization of the Channel of the Rio Grande from Caballo Dam to Fort Quitman, New Mexico-Texas," Symposium on Channel Stabilization Problems, Technical

  18. 76 FR 27281 - Airworthiness Directives; Dowty Propellers Type R212/4-30-4/22 and R251/4-30-4/49 Propeller...

    Science.gov (United States)

    2011-05-11

    ..., Aerospace Engineer, Boston Aircraft Certification Office, FAA, Engine and Propeller Directorate, 12 New... Aircraft Certification Office, FAA, Engine and Propeller Directorate, 12 New England Executive Park..., Acting Manager, Engine and Propeller Directorate, Aircraft Certification Service. BILLING CODE 4910-13-P...

  19. 76 FR 61558 - Airworthiness Directives; Dowty Propellers Type R212/4-30-4/22 and R251/4-30-4/49 Propeller...

    Science.gov (United States)

    2011-10-05

    ..., Aerospace Engineer, Boston Aircraft Certification Office, FAA, Engine & Propeller Directorate, 12 New..., Manager, Engine & Propeller Directorate, Aircraft Certification Service. BILLING CODE 4910-13-P ...-01-AD; Amendment 39-16807; AD 2011-19-02] RIN 2120-AA64 Airworthiness Directives; Dowty...

  20. The influence of oral and written information on antiretroviral drugs in the knowledge of users with HIV/AIDS - doi:10.5020/18061230.2010.p251

    Directory of Open Access Journals (Sweden)

    Regina Flávia de Castro Almeida

    2012-01-01

    Full Text Available Objective: To assess the influence of oral and written transmission of information on antiretroviral drugs in knowledge generation in their users and in the retention of information by them. Method: In the first phase, 18 individuals with HIV/AIDS treated at a referral hospital analyzed three brochures containing information on antiretroviral drugs and chose the best. In the second phase, three groups of 47 individuals with HIV/AIDS who received antiretroviral drugs in the same hospital were formed. The first group, considered the control group (group “C” received their medication at the pharmacy as usual, without any additional information; the second group (group “F” received a brochure with information about the drug in use, which should be read at that moment; and the third group (group “O” received orally, the same information detailed in the brochure. All answered a questionnaire that assessed their knowledge on the referred drug. Results: The responses of group “O” had a higher level of agreement with the information they received regarding action of the drug in the body (78.7%, duration of treatment (83%, procedure when missing a dose (91.5% and storage (95.7%. Conclusion: The transmission of information, whether oral or written, generates knowledge and the instructions and information when orally transmitted, in a detailed manner and in appropriate language, are more easily understood and assimilated. It appears also that, in the studied group, oral information resulted more immediately effective than written one.

  1. The Streambank Erosion Control Evaluation and Demonstration Act of 1974, Section 32, Public Law 93-251. Appendix D. Ohio River Demonstration Projects.

    Science.gov (United States)

    1981-12-01

    SERICCA 20 MOUNDSVILLE, W. VA. PHALARIS ARUNOINACEA REED CANARY GRASS 2 IT 01AGROSTIS AL&A "to TOP 5S T LOTUS CORNICULATUS ISIROSFOOT TRFIL 10 OHIO... CORNICULATUS BIROSFOOT TREFOIL to F ,*21 LAETPESTUCA ARUNOHNAC A TALL fESCUE 0S I TE LOTUS CORNICULATUS SIROSFOOT TREFOIL .00H1 RIVE PLATE 19 D-2-37...AGROS-ris .4LBA KED 7TOP s LorLJS CORNICULATUS AJ*DSFooT rXEF’OL 10 C FKSruc9 fiRuNDINACER, TALL FffSC IAf CPCV.31,VLTA) 30 CORrD/VILq4 Vh9#9PffYC.is

  2. The Streambank Erosion Control Evaluation and Demonstration Act of 1974, Section 32, Public Law 93-251. Appendix E. Missouri River Demonstration Projects. Volume 1.

    Science.gov (United States)

    1981-12-01

    Peachleaf Willow Salix amygdaloides -. Cottonwood Populus deltoides Russian Olive Elaeagnus angustifolia Buffaloberry Shepherdia argentea In addition, 8,000... Elaeagnus angustifolia Buffaloberry Shepherdia argentea Red-Osier Dogwood Cornus stolonifera Golden Willow Salix alba vitelina Hackberry Celtis

  3. Ship Trackline Along Which Continuous Resistivity Profile (CRP) Data was Collected in the Potomac River/Chesapeake Bay on Sept. 8, 2006 (RESGPSLNS_JD251.SHP)

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — In order to test hypotheses about groundwater flow under and into Chesapeake Bay, geophysical surveys were conducted by U.S. Geological Survey (USGS) scientists on...

  4. The introduction period of unicompartmental knee arthroplasty is critical: a clinical, clinical multicentered, and radiostereometric study of 251 Duracon unicompartmental knee arthroplasties.

    Science.gov (United States)

    Lindstrand, A; Stenström, A; Ryd, L; Toksvig-Larsen, S

    2000-08-01

    One hundred twenty-eight consecutive knees were operated on with the Duracon unicompartmental knee arthroplasty. Of 111 knees, followed 3 years (range, 1-6 years), 109 knees were satisfactory. Two knees were revised because of progression of osteoarthritis and inexplicable pain. Radiostereometric analysis in 49 knees showed a migration of 0.6 mm after 2 years. The magnitude of migration was lower in comparison with published series. In a multicenter study comprising 4 other hospitals, there were 8 revisions in 123 operated knees. The reasons were loosening, subsidence, or fracture. These revisions were within 1 year and mostly related to operative technique. Unicompartmental knee arthroplasty is a demanding procedure that needs special experience and includes a risk of early failures during the introduction of a system.

  5. http://periodicos.ufsc.br/index.php/gual/article/view/1983-4535.2012v5n2p251/22582

    Directory of Open Access Journals (Sweden)

    Alexandre Costa Quintana

    2012-08-01

    Full Text Available The process of virtual learning passes for the necessity of the existence of an evaluation process. Normally, this evaluation is integrated by a virtual part and another class attendance. The posting of the task becomes an important communication tool between students, teachers and tutors, as it enables the exchange of information between those involved, besides serving as a component of assessment and therefore reflecting the development of teaching-learning process.In this direction the objective of this work is in the distance to analyze the form of execution of the virtual tasks of the pupil of the course of graduation in Administration at distance. This work was elaborated, by means of a descriptive research, with analysis of content and a qualitative boarding. Concluding that the results of this study show that teachers, tutors and coordination of distance courses have attention on the needs of students in relation to time for implementation, appropriate date for posting and service to academics, that in understanding these will be key to the tasks are actually an important tool in the teaching-learning.

  6. Discovery of Isotopes of the Transuranium Elements with 93 <= Z <= 98

    CERN Document Server

    Fry, C

    2012-01-01

    One hundred and five isotopes of the transuranium elements neptunium, plutonium, americium, curium, berkelium and californium have so far been observed; the discovery of these isotopes is discussed. For each isotope a brief summary of the first refereed publication, including the production and identification method, is presented.

  7. RESGPSPNTS_JD251.SHP: Navigation, Bathymetry and Temperature Point at the Ship Position During Continuous Resistivity Profile Data Collection in the Potomac River/Chesapeake Bay on Sept. 8, 2006 on USGS Cruise 06018

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — In order to test hypotheses about groundwater flow under and into Chesapeake Bay, geophysical surveys were conducted by U.S. Geological Survey (USGS) scientists on...

  8. Graciela Speranza. Primera persona. Conversaciones con quince narradores argentinos. Fotografías de Alejandra López : Buenos Aires, Norma, 1995, 251 páginas

    OpenAIRE

    1996-01-01

    El libro de Graciela Speranza se inserta en un conjunto de publicaciones de crítica literaria argentina producidas en un espacio académico que a esta altura ya ha legitimado ciertas prácticas renovadoras. Dicho espacio no se mostró ajeno a los circuitos de publicación de los medios gráficos, y puede decirse que Primera persona es una muestra de ello. En efecto, la motivación de muchas conversaciones con escritores presentes en el libro de Graciela Speranza proviene de una idea periodística: p...

  9. GIS AND REMOTE SENSING BASEDLAND USE LAND COVER DYNAMICS AT SEMIEN MOUNTAIS NATIONAL PARK Authors name: Gebreanenya Gebru Kidane, Email. gglove2000@gmail.com University of Gondar Phone.251 920 88 84 62

    Science.gov (United States)

    Kidane, G. G., III; Belay, Y. G.; Kassa, B. A.; Yimam, D. A.

    2015-12-01

    ABSTRACT The main purpose of this study is to quantify the magnitude and rate of change of major land use/land cover types, and to identify the major drivers of change in SMNP using GIS and remote sensing. To address the pre-stated objective three landsat images of the periods between 1985, 2000 and 2015 with the time series of 15 (fifteen) years for the conceqative of 30 years land use land cover dynamics were classified and analyzed using Erdas Emagine 9.2 and ArcGIS 10 environments. The results depicted that a remarkable expansion was observed in forest cover followed by farmland and settlements between 1985 and 2015 by about 16% (22 ha/y) and 14.7% (21 ha/y) although some portions of their original extent were converted into other LUC classes. Expansion of forest cover was dominantly attributed to conversions of exposed rocks (1334.97 ha) and shrubs (1255.23 ha). These possibly attributed to conversion of some portions of exposed rocks and shrubs which were unsustainably utilized into protected areas by area closure and transformation of shrubs into forest cover. Similarly, farmlands and settlement has been expanded mainly due to area gains from grassland (984.51 ha) and forest cover (1037.25 ha). These largely associated with encroachment of farmlands and settlements into grasslands and original natural forest cover as a result of population pressure. The results also indicated that the reduction of areas under grassland, exposed volcanic rocks and shrubs by about 2.35% (5 ha/y), 18% (32 ha/y) and 11% (14 ha/y) evident because the added areas from other LUC classes could not compensate the losses, respectively. The major drivers identified in study area were civil war between 1984 and 1991 resulted in the agricultural exploitation, deforestation and illegal wildlife hunting, population pressure due to influx of settlers for the last decade. Finally revitalizing the nationally park is not only the duty of the government but also all the concerned individuals specially the communities around the park and different stock holders at different levels should be work in integrative and in synergism manner. Key Words: Dynamics, Land use, GIS, Remo sensing Email address of the authors: * gglove2000@gmail.com.

  10. A Novel Splice-Site Mutation in Angiotensin I-Converting Enzyme (ACE) Gene, c.3691+1G > A (IVS25+1G > A), Causes a Dramatic Increase in Circulating ACE through Deletion of the Transmembrane Anchor

    NARCIS (Netherlands)

    Persu, Alexandre; Lambert, Michel; Deinum, Jaap; Cossu, Marta; de Visscher, Nathalie; Irenge, Leonid; Ambroise, Jerome; Minon, Jean-Marc; Nesterovitch, Andrew B.; Churbanov, Alexander; Popova, Isolda A.; Danilov, Sergei M.; Danser, A. H. Jan; Gala, Jean-Luc

    2013-01-01

    Background: Angiotensin-converting enzyme (ACE) (EC 4.15.1) metabolizes many biologically active peptides and plays a key role in blood pressure regulation and vascular remodeling. Elevated ACE levels are associated with different cardiovascular and respiratory diseases. Methods and Results: Two Bel

  11. Jacques Dubois, Stendhal une sociologie romanesque, Paris, éditions la découverte, “textes à l’appui / laboratoire des sciences sociales”, 2007, 251 p.

    Directory of Open Access Journals (Sweden)

    Luciano Curreri

    2007-06-01

    Full Text Available Leonardo Sciascia, écrivain du social, de la politique, du pouvoir, a dit : « celui qui aime Stendhal ne peut pas aimer Proust ». Et Sciascia, en tant qu’écrivain du social, de la politique, du pouvoir, avoue à plusieurs reprises aimer Stendhal : Stendhal for ever est le titre d’une section de Faits divers d’histoire littéraire et civile (1989. Jacques Dubois, qui a étudié Proust et son sens du social, le sait très bien. Ce n’est pas un hasard si Stendhal une sociologie romanesque commence a...

  12. Synthesis of polymer materials by low energy electron beam. IV. EB-polymerized urethane-acrylate, -methacrylate and -acrylamide

    Science.gov (United States)

    Ando, Masayuki; Uryu, Toshiyuki

    The structure and properties before and after electron beam (EB) irradiation were investigated using urethane prepolymers with different terminal groups of 2-hydroxyethyl acrylate (HEA), 2-hydroxyethyl methacrylate (HEMA) and N-hydroxymethyl acrylamide (HMAAm). The prepolymers were synthesized by reaction of HEA, HEMA and HMAAm with the isocyanate-capped intermediate, which was obtained by reaction of poly(butylene adipate)diol (PBAD) with 4,4'-diphenylmethane diisocyanate. The resulting urethane-acrylate (UA-251M), -methacrylate (UMA-251M) and -acrylamide (UNAA-251M) had the crystallinity arising from PBAD moieties, and UA-251M and UMA-251M had higher crystallinity than UNAA-251M. IR results indicated that UNAA-251M was larger in the fraction of free NH stretching absorption than UA-251M and UMA-251M regardless of the number of NH group per a molecule. Accordingly, it was assumed that the difference in crystallinity was attributed to the polarity of terminal group. Hence, the rate of gel formation for UA-251M and UMA-251M was higher than that of UNAA-251M. The crystallinity based on PBAD of the prepolymers was remained also after EB irradiation. Spherulitic texture was observed on the EB-polymerized gel film surfaces for UA-251M and UMA-251M, while it was almost destroyed for UNAA-251M. Mechanical properties of UA-251M and UMA-251M gel films were much superior to those of UNAA-251M gel film according to the phase structure. Especially, UMA-251M gel film represented most excellent mechanical properties. Schematic models of the phase structure for UA-251M, UMA-251M and UNAA-251M were suggested from all experimental results.

  13. 76 FR 30394 - Amended Certification Regarding Eligibility To Apply for Worker Adjustment Assistance

    Science.gov (United States)

    2011-05-25

    ... LLC Lanham, Maryland TA-W-72,251K SUPERVALU, Inc. IT and Finance Departments Including Workers Whose..., Colorado (TA-W-72,251I); SUPERVALU, Inc., IT and Finance Departments, Lanham, Maryland (TA-W-72,251J...., and American Drug Stores LLC, Lanham, Maryland (TA-W-72,251J); SUPERVALU, Inc., IT and...

  14. TOWARD AN IMPROVED UNDERSTANDING OF STRUCTURE AND MAGNETISM IN NEPTUNIUM AND PLUTONIUM PHOSPHONATES AND SULFONATES

    Energy Technology Data Exchange (ETDEWEB)

    Albrecht-Schmitt, Thomas

    2012-03-01

    This grant supported the exploratory synthesis of new actinide materials with all of the actinides from thorium to californium with the exceptions of protactinium and berkelium. We developed detailed structure-property relationships that allowed for the identification of novel materials with selective ion-exchange, selective oxidation, and long-range magnetic ordering. We found novel bonding motifs and identified periodic trends across the actinide series. We identified structural building units that would lead to desired structural features and novel topologies. We also characterized many different spectroscopic trends across the actinide series. The grant support the preparation of approximately 1200 new compounds all of which were structurally characterized.

  15. Detection of rare earth elements in Powder River Basin sub-bituminous coal ash using laser-induced breakdown spectroscopy (LIBS)

    Energy Technology Data Exchange (ETDEWEB)

    Tran, Phuoc [National Energy Technology Lab. (NETL), Pittsburgh, PA, (United State; Mcintyre, Dustin [National Energy Technology Lab. (NETL), Pittsburgh, PA, (United State

    2015-10-01

    We reported our preliminary results on the use of laser-induced breakdown spectroscopy to analyze the rare earth elements contained in ash samples from Powder River Basin sub-bituminous coal (PRB-coal). We have identified many elements in the lanthanide series (cerium, europium, holmium, lanthanum, lutetium, praseodymium, promethium, samarium, terbium, ytterbium) and some elements in the actinide series (actinium, thorium, uranium, plutonium, berkelium, californium) in the ash samples. In addition, various metals were also seen to present in the ash samples

  16. PROCEEDINGS OF THE SYMPOSIUM COMMEMORATING THE 25th ANNIVERSARY OF ELEMENTS 97 and 98 HELD ON JAN. 20, 1975

    Energy Technology Data Exchange (ETDEWEB)

    Seaborg, Glenn T.; Street Jr., Kenneth; Thompson, Stanley G.; Ghiorso, Albert

    1976-07-01

    This volume includes the talks given on January 20, 1975, at a symposium in Berkeley on the occasion of the celebration of the 25th anniversary of the discovery of berkelium and californium. Talks were given at this symposium by the four people involved in the discovery of these elements and by a number of people who have made significant contributions in the intervening years to the investigation of their nuclear and chemical properties. The papers are being published here, without editing, in the form in which they were submitted by the authors in the months following the anniversary symposium, and they reflect rather faithfully the remarks made on that occasion.

  17. A Novel Splice-Site Mutation in Angiotensin I-Converting Enzyme (ACE) Gene, c.3691+1G.A (IVS25+1G.A), Causes a Dramatic Increase in Circulating ACE through Deletion of the Transmembrane Anchor

    NARCIS (Netherlands)

    Persu, A.; Lambert, M.; Deinum, J.; Cossu, M.; Visscher, N. de; Irenge, L.; Ambroise, J.; Minon, J.M.; Nesterovitch, A.B.; Churbanov, A.; Popova, I.A.; Danilov, S.M.; Danser, A.H.; Gala, J.L.

    2013-01-01

    BACKGROUND: Angiotensin-converting enzyme (ACE) (EC 4.15.1) metabolizes many biologically active peptides and plays a key role in blood pressure regulation and vascular remodeling. Elevated ACE levels are associated with different cardiovascular and respiratory diseases. METHODS AND RESULTS: Two Bel

  18. Physical trajectory profile data from glider unit_251 deployed by Oregon Health & Science University; Center for Coastal Margin Observation & Prediction in the Coastal Waters of Washington/Oregon from 2013-07-18 to 2013-08-06 (NCEI Accession 0145922)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Slocum glider dataset gathered as part of the CMOP observatory SATURN, funded by NOAA and NSF. Glider operations have historically been seasonal (April-September),...

  19. The Launch of a Joint Library/Writing Centre Online Course on Academic Integrity. A Review of: Greer, K., Swanberg, S., Hristova, M., Switzer, A. T., Daniel, D., & Perdue, S. W. (2012. Beyond the web tutorial: Development and implementation of an online, self-directed academic integrity course at Oakland University. The Journal of Academic Librarianship, 38(5, 251-258.

    Directory of Open Access Journals (Sweden)

    Cari Merkley

    2013-06-01

    Full Text Available Objective – To outline the collaborative development of an online course addressing academic integrity by a university’s library system and writing centre.Design – Case study.Setting – A public research university in the Midwestern United States.Subjects – 1650 students who completed the online module.Methods – Oakland University (OU Libraries and the Writing Centre began to collaborate on the development of a new online course on academic integrity in 2011. It was felt that an existing online library tutorial on plagiarism no longer met the needs of students and faculty. The development of the course was informed by the Association of College and Research Libraries’ Information Literacy Competency Standards for Higher Education (2000 as well as a research study investigating students’ use of sources in their scholarly writing across several institutions. Moodle, the institution’s learning management system (LMS, was used to develop the learning object.Main Results – OU Libraries and the Writing Centre launched the six-part online course entitled “Using and Citing Sources” in January 2012. They developed modules around learning outcomes in five broad categories: defining academic integrity and plagiarism; the use of sources in academic writing; paraphrasing; quoting; and citation. The final module provided students with an opportunity to practise lessons learned in the first five modules. The use of the LMS to design and host the course limited the tutorial to registered students, but provided developers with access to additional course functionality without labour-intensive coding. It also allowed Writing Centre staff to access students’ performance data on the modules prior to their appointments. Improvements over the previous online tutorial included expanded content on academic ethics and referencing, more active learning elements, video content, and the opportunity for students to choose discipline-specific examples. In the first four months of its availability, 1650 students completed the course, with 3330 attempts overall.Conclusion – The diverse perspectives and expertise that individuals from OU Libraries and the Writing Center brought to their collaboration greatly informed the development of the course. The time and effort saved by using the university’s existing LMS to develop interactive content and the focus on providing students with opportunities for active learning within the course contributed to the project’s success.

  20. Physical trajectory profile data from glider unit_251 deployed by Oregon Health & Science University; Center for Coastal Margin Observation & Prediction in the Coastal Waters of Washington/Oregon from 2012-07-25 to 2012-08-22 (NCEI Accession 0145920)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Slocum glider dataset gathered as part of the CMOP observatory SATURN, funded by NOAA and NSF. Glider operations have historically been seasonal (April-September),...

  1. Dietary exposure to benzoates (E210-E213), parabens (E214-E219), nitrites (E249-E250), nitrates (E251-E252), BHA (E320), BHT (E321) and aspartame (E951) in children less than 3 years old in France.

    Science.gov (United States)

    Mancini, F R; Paul, D; Gauvreau, J; Volatier, J L; Vin, K; Hulin, M

    2015-01-01

    This study aimed to estimate the exposure to seven additives (benzoates, parabens, nitrites, nitrates, BHA, BHT and aspartame) in children aged less than 3 years old in France. A conservative approach, combining individual consumption data with maximum permitted levels, was carried out for all the additives. More refined estimates using occurrence data obtained from products' labels (collected by the French Observatory of Food Quality) were conducted for those additives that exceeded the acceptable daily intake (ADI). Information on additives' occurrence was obtained from the food labels. When the ADI was still exceeded, the exposure estimate was further refined using measured concentration data, if available. When using the maximum permitted level (MPL), the ADI was exceeded for benzoates (1.94 mg kg(-1) bw day(-1)), nitrites (0.09 mg kg(-1) bw day(-1)) and BHA (0.39 mg kg(-1) bw day(-1)) in 25%, 54% and 20% of the entire study population respectively. The main food contributors identified with this approach were current foods as these additives are not authorised in specific infant food: vegetable soups and broths for both benzoates and BHA, delicatessen and meat for nitrites. The exposure estimate was significantly reduced when using occurrence data, but in the upper-bound scenario the ADI was still exceeded significantly by the age group 13-36 months for benzoates (2%) and BHA (1%), and by the age group 7-12 months (16%) and 13-36 months (58%) for nitrites. Measured concentration data were available exclusively for nitrites and the results obtained using these data showed that the nitrites' intake was below the ADI for all the population considered in this study. These results suggest that refinement of exposure, based on the assessment of food levels, is needed to estimate the exposure of children to BHA and benzoates for which the risk of exceeding the ADI cannot be excluded when using occurrence data.

  2. Physical trajectory profile data from glider unit_251 deployed by Oregon Health & Science University; Center for Coastal Margin Observation & Prediction in the Coastal Waters of Washington/Oregon from 2013-05-08 to 2013-05-31 (NCEI Accession 0145921)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Slocum glider dataset gathered as part of the CMOP observatory SATURN, funded by NOAA and NSF. Glider operations have historically been seasonal (April-September),...

  3. Dicty_cDB: Contig-U08414-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available gtaagtggtatttcaaaagcatgntnccttttaaat 190 Query: 191 nnaattttcanagcttctcccgacactanaggatttgcnattnnatgtggtactng...ggat 250 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct: 191 nnaattttcan...|||||||||||||||||||||||||||||||||| Sbjct: 95 naattttcanagcttctcccgacactanaggatttg...|||||||||||||||||||||||||||||||||||||| Sbjct: 155 ataccanntcaatcgataatggtgtaagtggtatttcaaaagcatgntnccttttaaatn 96 Query: 192 naattttc...anagcttctcccgacactanaggatttgcnattnnatgtggtactngggatg 251 ||||||||||||||||||||||||||

  4. 47 CFR 1.1204 - Exempt ex parte presentations and proceedings.

    Science.gov (United States)

    2010-10-01

    ... agency or branch and the Commission share jurisdiction provided that, any new factual information.... 251(e); (iii) The Universal Service Administrative Company relating to the administration of...

  5. Nuclear fission and the transuranium elements

    Energy Technology Data Exchange (ETDEWEB)

    Seaborg, G.T.

    1989-02-01

    Many of the transuranium elements are produced and isolated in large quantities through the use of neutrons furnished by nuclear fission reactions: plutonium (atomic number 94) in ton quantities; neptunium (93), americium (95), and curium (96) in kilogram quantities; berkelium (97) in 100 milligram quantities; californium (98) in gram quantities; and einsteinium (99) in milligram quantities. Transuranium isotopes have found many practical applications---as nuclear fuel for the large-scale generation of electricity, as compact, long-lived power sources for use in space exploration, as means for diagnosis and treatment in the medical area, and as tools in numerous industrial processes. Of particular interest is the unusual chemistry and impact of these heaviest elements on the periodic table. This account will feature these aspects. 9 refs., 5 figs.

  6. Review: Somchai Phatharathananunth (2006, Civil Society and Democratization. Social Movements in Northeast Thailand Buchbesprechung: Somchai Phatharathananunth (2006, Civil Society and Democratization. Social Movements in Northeast Thailand

    Directory of Open Access Journals (Sweden)

    Wolfram Schaffar

    2009-10-01

    Full Text Available Book Review of Somchai Phatharathananunth (2006, Civil Society and Democratization. Social Movements in Northeast Thailand, Copenhagen: NIAS Press; ISBN 10: 8791114381, 251 pages. Buchbesprechung der Monografie: Somchai Phatharathananunth (2006, Civil Society and Democratization. Social Movements in Northeast Thailand, Copenhagen: NIAS Press; ISBN 10: 8791114381, 251 pages.

  7. 76 FR 41075 - Copyright Arbitration Royalty Panel Rules and Procedures

    Science.gov (United States)

    2011-07-13

    ... Copyright Office 37 CFR Part 251 Copyright Arbitration Royalty Panel Rules and Procedures AGENCY: Copyright... making an amendment to its regulations by removing Part 251 Copyright Arbitration Royalty Panel Rules of Procedure. In 2004, Congress replaced the Copyright Arbitration Royalty Panels with three Copyright...

  8. 75 FR 57504 - Amended Certification Regarding Eligibility To Apply for Worker Adjustment Assistance

    Science.gov (United States)

    2010-09-21

    ... New Albertsons, Inc., Lanham, Maryland SUPERVALU, Inc., IT and Finance Departments, Including Workers...,251I); SUPERVALU, Inc., IT and Finance Departments, Lanham, Maryland (TA-W- 72,251J); SUPERVALU, Inc..., including workers whose unemployment insurance (UI) wages are paid through New Albertsons, Inc.,...

  9. Dynamics of the proteome in human and farm animal milk

    NARCIS (Netherlands)

    Zhang, L.

    2015-01-01

    25.1pt;">Abstract

    25.1pt;">The milk proteome changes due to many factors, such as lactation, individual, health status, processing, and species differences. The objective of the work described in this thesis was to increase our understan

  10. Musik som analogi og metafor

    DEFF Research Database (Denmark)

    2014-01-01

    Indeholder underkapitlerne: 2.5.1 Musik som analogi 2.5.2 Musik som metafor 2.5.3 Musikkens psykologiske funktioner - en taxonomi og metaforisk lytning til fire baroksatser......Indeholder underkapitlerne: 2.5.1 Musik som analogi 2.5.2 Musik som metafor 2.5.3 Musikkens psykologiske funktioner - en taxonomi og metaforisk lytning til fire baroksatser...

  11. EST Table: FS733896 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available FS733896 E_FL_bmmt_22L01_F_0 10/09/28 30 %/251 aa ref|XP_974925.1| PREDICTED: similar to Juvenile.../10 low homology 10/09/10 30 %/251 aa gi|91080979|ref|XP_974925.1| PREDICTED: similar to Juvenile hormone-inducible protein, putative [Tribolium castaneum] FS725285 bmmt ...

  12. Fast Neutron Induced Autophagy Leads To Necrosis In Glioblastoma Multiforme Cells

    Science.gov (United States)

    Yasui, Linda; Gladden, Samantha; Andorf, Christine; Kroc, Thomas

    2011-06-01

    Fast neutrons are highly effective at killing glioblastoma multiforme (GBM), U87 and U251 cells. The mode of cell death was investigated using transmission electron microscopy (TEM) to identify the fraction of irradiated U87 or U251 cells having morphological features of autophagy and/or necrosis. U87 or U251 cells were irradiated with 2 Gy fast neturons or 10 Gy γ rays. A majority of U87 and U251 cells exhibit features of cell death with autophagy after irradiation with either 10 Gy γ rays or 2 Gy fast neutrons. Very few γ irradiated cells had features of necrosis (U87 or U251 cell samples processed for TEM 1 day after 10 Gy γ irradiation). In contrast, a significant increase was observed in necrotic U87 and U251 cells irradiated with fast neutrons. These results show a greater percentage of cells exhibit morphological evidence of necrosis induced by a lower dose of fast neutron irradiation compared to γ irradiation. Also, the evidence of necrosis in fast neutron irradiated U87 and U251 cells occurs in a background of autophagy. Since autophagy is observed before necrosis, autophagy may play a role in signaling programmed necrosis in fast neutron irradiated U87 and U251 cells.

  13. Dicty_cDB: SSM325 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available e... 252 8e-66 AF242310_1( AF242310 |pid:none) Euphorbia esula manganese superoxi... 251 1e-65 AY675508_1( AY675508 |pid:none) Parago...nimus westermani Mn-SOD mRNA... 251 2e-65 protein update 2009. 6.15 PSORT psg: 0.73

  14. Processes determining seasonality and interannual variability of settling particle fluxes to the deep Arabian Sea

    Digital Repository Service at National Institute of Oceanography (India)

    Haake, B.; Rixen, T.; Reemtsma, T.; Ramaswamy, V.; Ittekkot, V.

    stream_size 20 stream_content_type text/plain stream_name Particle_Flux_Ocean_Chapter_14_1996_251.pdf.txt stream_source_info Particle_Flux_Ocean_Chapter_14_1996_251.pdf.txt Content-Encoding ISO-8859-1 Content-Type text...

  15. Review: Derek Hook, A Critical Psychology of the Postcolonial: The Mind of Apartheid (2012 Buchbesprechung: Derek Hook, A Critical Psychology of the Postcolonial: The Mind of Apartheid (2012

    Directory of Open Access Journals (Sweden)

    Danielle Faye Tran

    2013-01-01

    Full Text Available Review of the monograph:Derek Hook, A Critical Psychology of the Postcolonial: The Mind of Apartheid, London: Routledge, 2012, ISBN 978-0-415-58757-0, 251 pagesBesprechung der Monographie:Derek Hook, A Critical Psychology of the Postcolonial: The Mind of Apartheid, London: Routledge, 2012, ISBN 978-0-415-58757-0, 251 Seiten

  16. Gclust Server: 119743 [Gclust Server

    Lifescience Database Archive (English)

    Full Text Available 119743 CEL_K06A5.7_17506269 Cluster Sequences Related Sequences(24) 604 cdc-25.1: Cell Division...nces Related Sequences(24) Sequence length 604 Representative annotation cdc-25.1: Cell Division

  17. 75 FR 27002 - Petitions for Modification

    Science.gov (United States)

    2010-05-13

    ... bags, rock dust, mine gob, earth--rock and dirt, gravel, refuse, or other similar material that is...) . Modification Request: The petitioner proposes to use Model J251-D/E Face Drill, manufactured by J.H. Fletcher & Company. The petitioner states that: (1) The J251-D/E Face Drill consists of an electric drill...

  18. Carboplatin and taxol resistance develops more rapidly in functional BRCA1 compared to dysfunctional BRCA1 ovarian cancer cells.

    Science.gov (United States)

    Busschots, Steven; O'Toole, Sharon; O'Leary, John J; Stordal, Britta

    2015-08-01

    A major risk factor for ovarian cancer is germline mutations of BRCA1/2. It has been found that (80%) of cellular models with acquired platinum or taxane resistance display an inverse resistance relationship, that is collateral sensitivity to the other agent. We used a clinically relevant comparative selection strategy to develop novel chemoresistant cell lines which aim to investigate the mechanisms of resistance that arise from different exposures of carboplatin and taxol on cells having BRCA1 function (UPN251) or dysfunction (OVCAR8). Resistance to carboplatin and taxol developed quicker and more stably in UPN251 (BRCA1-wildtype) compared to OVCAR8 (BRCA1-methylated). Alternating carboplatin and taxol treatment delayed but did not prevent resistance development when compared to single-agent administration. Interestingly, the sequence of drug exposure influenced the resistance mechanism produced. UPN251-6CALT (carboplatin first) and UPN251-6TALT (taxol first) have different profiles of cross resistance. UPN251-6CALT displays significant resistance to CuSO4 (2.3-fold, p=0.004) while UPN251-6TALT shows significant sensitivity to oxaliplatin (0.6-fold, p=0.01). P-glycoprotein is the main mechanism of taxol resistance found in the UPN251 taxane-resistant sublines. UPN251 cells increase cellular glutathione levels (3.0-fold, p=0.02) in response to carboplatin treatment. However, increased glutathione is not maintained in the carboplatin-resistant sublines. UPN251-7C and UPN251-6CALT are low-level resistant to CuSO4 suggesting alterations in copper metabolism. However, none of the UPN251 sublines have alterations in the protein expression of ATP7A or CTR1. The protein expression of BRCA1 and MRP2 is unchanged in the UPN251 sublines. The UPN251 sublines remain sensitive to parp inhibitors veliparib and CEP8983 suggesting that these agents are candidates for the treatment of platinum/taxane resistant ovarian cancer patients.

  19. Arabidopsis CDS blastp result: AK121915 [KOME

    Lifescience Database Archive (English)

    Full Text Available ant defensin protein family member, personal communication, Bart Thomma (このメールアドレスはスパムボットから保護されています。閲覧するにはJavaScriptを有効にする必要があります。 document.getElementById('cloakba928d15337a4bca55b45e1edae4f251').innerHTML = ''; var prefix = 'ma' + 'il' + 'to'; var path = 'hr' + 'ef' + '='; var addyba928d15337a4bca55b45e1edae4f251 = 'Bart.Thomma' + '@'; addyba928d15337a4bca55b45e1edae4f251 = addyba928d15337a4bca55b45e1edae4f251 + 'agr' + '.' + 'kuleuven' + '.' + 'ac' + '.' + 'be'; var addy_textba928d15337a4bca55b45e1edae4f251 = 'Bart.Thomma' + '@' + 'agr' + '.' + 'kuleuven' + '.' + 'ac' + '.' + 'be';document.getElementById('cloakba928d15337a4bca55b45e1edae4f251').innerHTML += ''+addy_textba928d15337a4bca55b45e1edae4f251+''; ); similar to SWISS-PROT:O65740 9e-18 ...

  20. A recombinant carboxy-terminal domain of alpha-toxin protects mice against Clostridium perfringens.

    Science.gov (United States)

    Nagahama, Masahiro; Oda, Masataka; Kobayashi, Keiko; Ochi, Sadayuki; Takagishi, Teruhisa; Shibutani, Masahiro; Sakurai, Jun

    2013-05-01

    Clostridium perfringens alpha-toxin (CP, 370 residues) is one of the main agents involved in the development of gas gangrene. In this study, the immunogenicity and protective efficacy of the C-terminal domain (CP251-370) of the toxin and phospholipase C (PLC; CB, 372 residues) of Clostridum bifermentans isolated from cases of clostridium necrosis were examined. The recombinant proteins were expressed as glutathione S-transferase (GST) fusion proteins. Antibodies that cross-reacted with alpha-toxin were produced after immunization with recombinant proteins including GST-CP251-370, GST-CP281-370, GST-CP311-370, CB1-372 and GST-CB251-372. Anti-GST-CP251-370, anti-GST-CP281-370 and anti-GST-CP311-370 sera neutralized both the PLC and hemolytic activities of alpha-toxin, whereas anti-CB1-372 and anti-GST-CB251-372 weakly neutralized these activities. Immunization with GST-CP251-370 and GST-CP281-370 provided protection against the lethal effects of the toxin and C. perfringens type A NCTC8237. Partial protection from the toxin and C. perfringens was elicited by immunization with GST-CP311-370 and CB1-372. GST-CP251-370 and GST-CP281-370 are promising candidates for vaccines for clostridial-induced gas gangrene.

  1. 40 CFR Appendix A to Subpart A of... - Switcher Locomotives

    Science.gov (United States)

    2010-07-01

    ... (2). ALCO and MLW S1 6-539NA. S2 6-539T. S3 6-539NA. S4 6-539T. S5 6-251. S6 6-251A,B. S7 6-539. S10 6-539. S11 6-539. S12 6-539T. S13 6-251C. RSD-1 6-539. RSC-13 6-539. RSC-24 12-244 RS1 6-539T. RS2...

  2. Monsoonal and fertility contols on recent marginal sea and continental shelf coccolith assemblages from the western Pacific and northern Indian Oceans

    Digital Repository Service at National Institute of Oceanography (India)

    Houghton, S.D.; Guptha, M.V.S.

    stream_size 35380 stream_content_type text/plain stream_name Mar_Geol_97_251.pdf.txt stream_source_info Mar_Geol_97_251.pdf.txt Content-Encoding UTF-8 Content-Type text/plain; charset=UTF-8 Marine Geology, 97 (1991) 251..., with English Summary). Eppley, R.W., Rogers, J.N. and McCarthy, J.J., 1969. Half- saturation constants for uptake of nitrate and ammonia by marine phytoplankton. Limnol. Oceanogr., 14: 912-920. Fairbridge, R.W., 1986. Monsoons and paleomonsoons. Epi- sodes...

  3. Naval Justice School Evidence Study Guide. Revision

    Science.gov (United States)

    1989-01-01

    exclusion sanction. Nardone v. United States, 308 U.S. 338 (1939); Silverthorne Lumber Co. v. United States, 251 U.S. 385 (1920). 2. The rationale for the...source" [see Silverthorne Lumber Co. v. United States, 251 U.S. 385 (1920)]; "attenuation" [see Wong Sun v. United States, 371 U.S. 471 (1963)]; or...Wong Sun v. United States, 371 U.S. 471 (1963); Silverthorne Lumber Co. v. United States, 251 U.S. 385 (1920). 2. In United States v. Crews, 445 U.S

  4. 76 FR 20957 - Gulf of Mexico Fishery Management Council; Public Meeting

    Science.gov (United States)

    2011-04-14

    ..., Orange Beach, FL 36561, telephone: (251) 543-4444; Wednesday, May 4, 2011 Amend 32--Banana Bay Resort...; Thursday, May 5, 2011 ACL--Banana Bay Resort, 4590 Overseas Highway, Marathon, FL 33050, telephone:...

  5. Drug: D00096 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available anal organ agents 251 Urinary organ agents 2519 Others D00096 D-Sorbitol (JP16); Sorbitol (NF) 7 Agents not mainly for therapeutic... purpose 79 Other agents not mainly for therapeutic purpose 799 Miscellaneous 7990 Mis

  6. 78 FR 77594 - Safety Zone; Barge Launches; Gulfport Lake; Gulfport, MS

    Science.gov (United States)

    2013-12-24

    ...: If you have questions on this rule, call or email LT Stanley A. Tarrant, Coast Guard Sector Mobile, Waterways Management Division; telephone (251) 441-5940, email Stanley.A.Tarrant@uscg.mil . If you...

  7. Aerodynamic Performance and Particle Image Velocimetery of Piezo Actuated Biomimetic Manduca Sexta Engineered Wings Towards the Design and Application of a Flapping Wing Flight Vehicle

    Science.gov (United States)

    2013-12-01

    2.5.1 Powerplant & Drive Train . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72 2.5.2 The AFIT FWMAV Design...51 Ftrans force due to translation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51 f wave ...36 ϕ signal wave phase shift . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 186 ψf feather angle

  8. Wide Output Range Power Processing Unit for Electric Propulsion Project

    Data.gov (United States)

    National Aeronautics and Space Administration — A power supply concept capable of operation over 25:1 and 64:1 impedance ranges at full power has been successfully demonstrated in our Phase I effort at...

  9. Immunodiagnostic Techniques for Bacterial Infections

    Science.gov (United States)

    1981-01-01

    capsulatuni Serum Actinomvcesr israeli Serum Aspergillus fumiqatus Serum Protozoan: Trvnanosoma cruzi Serum Entameaba histolvtica Serum Trichinella sviralis...serological study of human Trypanosoma rhodesiense infections using a microscale enzyme-linked immunosorbent assay. Tropenmed. Parasitol. 26:247-251, 1975

  10. Anime alate Ascensioni e ascesi in Fogazzaro

    Directory of Open Access Journals (Sweden)

    Sergio Facchetti

    2005-12-01

    Full Text Available Recensione di: Vincenzo Crupi, "Fra il cielo e l´inferno". Ascensioni umane nell´ultimo Fogazzaro, Soveria Mannelli (Catanzaro, Rubbettino Ediore, collana "Iride" fondata e diretta da Rocco Mario Morano, 2004, 251 p.

  11. 78 FR 2111 - California State Motor Vehicle Pollution Control Standards; Notice of Decision Granting a Waiver...

    Science.gov (United States)

    2013-01-09

    ...-2012-0562- 0372 which notes that ``Toyota could be forced to employ a variety of costly marketing... percent by MY 2025, and from about 251 grams of CO 2 per mile to 166 grams, based on the projected mix...

  12. QTL Information Table: 368 [Q-TARO

    Lifescience Database Archive (English)

    Full Text Available qPGWC-6b Physiological trait Eating quality Percentages of grains with chalkiness R...3). Mapping quantitative trait loci underlying appearance quality of rice grains (Oryza sativa L.). Yi Chuan Xue Bao 30, 251-259. ...

  13. QTL Information Table: 369 [Q-TARO

    Lifescience Database Archive (English)

    Full Text Available qET-6 Physiological trait Eating quality Endosperm translucency RFLP C)Interval BIL...tative trait loci underlying appearance quality of rice grains (Oryza sativa L.). Yi Chuan Xue Bao 30, 251-259. ...

  14. Calcium supplements

    Science.gov (United States)

    ... do not help. Always tell your provider and pharmacist if you are taking extra calcium. Calcium supplements ... 2012:chap 251. The National Osteoporosis Foundation (NOF). Clinician's Guide to prevention and treatment of osteoporosis . National ...

  15. 78 FR 50054 - Environmental Impacts Statements; Notice of Availability

    Science.gov (United States)

    2013-08-16

    ... Banks Coastal Storm Damage Reduction, Comment Period Ends: 09/30/2013, Contact: Eric Gasch 910-251-4553... Wullenjohn 928-328-6189. EIS No. 20130241, Final EIS, USACE, FL, Tarmac King Road Limestone Mine,...

  16. 13 CFR 315.8 - Processing petitions for certification.

    Science.gov (United States)

    2010-01-01

    ... petition has not been accepted, but may be resubmitted at any time without prejudice when the specified... determine whether the petitioner meets the requirements set forth in section 251(c) of the Trade Act...

  17. Data of evolutionary structure change: 1DJ2B-1ADIA [Confc[Archive

    Lifescience Database Archive (English)

    Full Text Available 1DJ2B-1ADIA 1DJ2 1ADI B A IGSLSQVSGVLGCQWGDEGKGKLVDILAQHFDIVARCQG...LLSEACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAEKLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADI...5 PHE CA 263 LYS CA 227 TYR CA 251 1ADI... A 1ADIA 1 1ADI A 1ADI

  18. TMFunction data: 603 [TMFunction[Archive

    Lifescience Database Archive (English)

    Full Text Available brio parahaemolyticus ... Otsuka M, Yasuda M, Morita Y, Otsuka C, Tsuchiya T, Omote H, Moriyama Y. J Bacteriol.... E251Q ... YES Na+ dependent drug transport process ... NorM Na+/ Multidrug antiporter Vi

  19. TMFunction data: 592 [TMFunction[Archive

    Lifescience Database Archive (English)

    Full Text Available brio parahaemolyticus ... Otsuka M, Yasuda M, Morita Y, Otsuka C, Tsuchiya T, Omote H, Moriyama Y. J Bacteriol.... E251Q ... YES Na+ dependent drug transport process ... NorM Na+/ Multidrug antiporter Vi

  20. TMFunction data: 602 [TMFunction[Archive

    Lifescience Database Archive (English)

    Full Text Available brio parahaemolyticus ... Otsuka M, Yasuda M, Morita Y, Otsuka C, Tsuchiya T, Omote H, Moriyama Y. J Bacteriol.... E251D ... YES Na+ dependent drug transport process ... NorM Na+/ Multidrug antiporter Vi

  1. TMFunction data: 612 [TMFunction[Archive

    Lifescience Database Archive (English)

    Full Text Available brio parahaemolyticus ... Otsuka M, Yasuda M, Morita Y, Otsuka C, Tsuchiya T, Omote H, Moriyama Y. J Bacteriol.... E251D ... YES Na+ dependent drug transport process ... NorM Na+/ Multidrug antiporter Vi

  2. TMFunction data: 591 [TMFunction[Archive

    Lifescience Database Archive (English)

    Full Text Available brio parahaemolyticus ... Otsuka M, Yasuda M, Morita Y, Otsuka C, Tsuchiya T, Omote H, Moriyama Y. J Bacteriol.... E251D ... YES Na+ dependent drug transport process ... NorM Na+/ Multidrug antiporter Vi

  3. TMFunction data: 613 [TMFunction[Archive

    Lifescience Database Archive (English)

    Full Text Available brio parahaemolyticus ... Otsuka M, Yasuda M, Morita Y, Otsuka C, Tsuchiya T, Omote H, Moriyama Y. J Bacteriol.... E251Q ... YES Na+ dependent drug transport process ... NorM Na+/ Multidrug antiporter Vi

  4. 芹菜素对胶质瘤细胞增殖抑制作用的研究

    Institute of Scientific and Technical Information of China (English)

    杨志英; 邓志刚; 伍健明; 蓝伟红

    2012-01-01

    Objective To investigate the inhibitory effect of apigenin on glioma U251 cells. Methods U251 cells were treated by apigenin with various concentrations ( 20、 40、 80 μmol/L) . Cell proliferation was measured by MTT assay on 24, 48 and 72 h. Results Apigenin inhibited the growth and proliferation of U251 cells in a dose-dependent and time-dependent manner. Inhibition of growth was observed on 24, 48 and 72 h at the concentration of 40 and 80 μmol/L, while at the concentration of 20 μ mol/L no effect was observed. Conclusion The results demonstrate that apigenin has potent inhibitory action on glioma cell growth and proliferation.%目的 探讨芹菜素对人胶质瘤细胞U251的增殖抑制作用.方法 用四甲基偶氮唑盐(MTT)比色法,检测不同浓度(20μmol/L、40μmol/L、80μmol/L)的芹菜素作用不同时间(24h、48h、72h),对人胶质瘤细胞株U251生长的影响.结果 低剂量的芹菜素(20μmol/L)无明显抑制胶质瘤U251细胞增殖的作用,而中剂量(40μmol/L)及高剂量的芹菜素(80μmol/L)能抑制胶质瘤U251细胞增殖,且抑制作用呈剂量和时间依赖性.结论 芹菜素有抑制人胶质瘤细胞U251增殖的作用.

  5. Handbook for Transformation of Datums, Projections, Grids, and Common Coordinate Systems

    Science.gov (United States)

    2004-05-01

    Publica - tion No. 251 Conformal Projections in Geodesy and Cartography. (Thomas, 1979) Engineer Technical Letter No. 1110-1-147 Engineering and...the NOAA Manual NOS NGS 5 and the Coast and Geodetic Survey Special Publica - tion No. 251 are available from National Geodetic Information Branch, N...Liberia (CD) 0 1987 -90 15 40 15 88 15 LUZON LUZ Clarke 1866 Philippines (Excluding Mindanao Island) LUZ -A (CC) 0 1987 -133 8 -77

  6. The Vertical Limit of State Sovereignty

    Science.gov (United States)

    2005-06-01

    degrees, 39 minutes, and 58 seconds East Longitude. See Federation of American Scientist , No-dong (last modified Mar. 25, 40 warnings of the...23, 2005) <http://www.fas.org/nuke/guide/dprk/facility/nodong.htm> [hereinafter Federation of American Scientist , No-dong]. 251 See Kazuhiro...claiming the rocket had two- stages). 253 See Nakatani, supra note 251, at 144; Warwick, supra note 252; Federation of American Scientist , No-dong

  7. The effect of transport density and gender on stress indicators and carcass and meat quality in pigs

    Energy Technology Data Exchange (ETDEWEB)

    Pereira, T.L.; Corassa, A.; Komiyama, C. M.; Araújo, C.V.; Kataoka, A.

    2015-07-01

    A total of 168 finishing pigs were used to investigate the effects of gender (barrows and gilts) and transport densities for slaughter (236, 251, and 275 kg/m²) on stress indicators and carcass and pork quality. The animals transported at 251 kg/m² (T251) presented cortisol values below those at 236 kg/m2 (T236), but no different from those at 275 kg/m2 (T275). The lactate dehydrogenase (LDH) values in pigs transported at T236 were the lowest. The blood components did not differ between T236 and T275. The pH values at 45 min (pH45) and at 24 h (pH24) postmortem were higher for pigs subjected to T236. However, the pH45 was higher at T251 than at T275, but pH24 was lower at T251 than at T275. The lightness values in the muscles of the pigs transported at T236 and T251 were higher than those at T275. Lower drip loss values were observed in the muscle of animals at T251. Carcasses of pigs at T236 contained more 1–5 cm lesions while those at T275 contained more 5–10 cmlesions in sections of loin. No significant effects of gender were found on the stress indicators, blood components, pH45, pH24, color, drip loss or carcass lesions in general. These results indicate that the pre-slaughter transport of pigs at densities of 251 kg/m² generates less physiological damage and smaller losses on carcass and pork quality irrespective of gender. (Author)

  8. Hospital Security and Force Protection: A Guide to Ensuring Patient and Employee Safety

    Science.gov (United States)

    2006-04-28

    removed from the mother baby unit. This technology also may be attached to equipment, decreasing theft of wheelchairs and other easily lost items (Noble... Pediatric Emergency Care, 19(4), 248-251. Hospital Security 93 State of Delaware. (2005). The history of terrorism: More than 200 years of development... Pediatric Emergency Care, 19(4), 248-251. Sullivan, M., & Donnelly, B. (2005). Emergency department response to terrorism. Topics in Emergency Medicine, 27

  9. Drug: D05017 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available ics Agents against Amebiasis and other antiprotozoals Nitroimidazole derivatives Metronidazole [ATC:A01AB17 ...D05017 Drug Metronidazole phosphate (USAN) C6H10N3O6P 251.0307 251.1339 D05017.gif Antibacterial; Antiprotoz...oal ATC code: A01AB17 D06BX01 G01AF01 J01XD01 P01AB01 Anatomical Therapeutic Chemic

  10. Report: Physics Constrained Stochastic Statistical Models for Extended Range Environmental Prediction

    Science.gov (United States)

    2013-09-30

    for Extended Range Environmental Prediction Andrew J. Majda New York University Courant Institute of Mathematical Sciences 251 Mercer Street...UCLA), and Dimitri Giannakis ( Courant Institute) with their post docs and Ph.D. students: A. Reemergence Mechanisms for North Pacific Sea Ice...ES) New York University, Courant Institute of Mathematical Sciences,251 Mercer Street,New York,NY,10012 8. PERFORMING ORGANIZATION REPORT NUMBER 9

  11. A Cross-Shore Model of Barrier Island Migration over a Compressible Substrate

    Science.gov (United States)

    2010-01-01

    substrate Marine Geology 271 (2010) 1–16 ⁎ Corresponding author. Tel.: +1 251 694 3719; fax: +1 251 690 3464. E-mail address: Julie.D.Rosati...ScienceDirect Marine Geology j ourna l homepage: www.e lsev ie r.com/ locate /margeo Report Documentation Page Form ApprovedOMB No. 0704-0188 Public reporting...Egypt (Stanley and Warne, 1998), the Ganges–Brahmaputra Rivers, Bangladesh , India (Allison, 1998); and the Yangtze River, China (Xiqing, 1998

  12. Book Review: Training the Party: Party Adaptation and Elite Training in Reform-era China

    DEFF Research Database (Denmark)

    Brødsgaard, Kjeld Erik

    2016-01-01

    Review of: Training the Party: Party Adaptation and Elite Training in Reform-era China. Charlotte P. Lee . Cambridge: Cambridge University Press, 2015. xii + 251 pp. $99.99. ISBN 978-1-107-09063-7......Review of: Training the Party: Party Adaptation and Elite Training in Reform-era China. Charlotte P. Lee . Cambridge: Cambridge University Press, 2015. xii + 251 pp. $99.99. ISBN 978-1-107-09063-7...

  13. Characterization of a Viking Blade Fabricated by Traditional Forging Techniques

    Science.gov (United States)

    Vo, H.; Frazer, D.; Bailey, N.; Traylor, R.; Austin, J.; Pringle, J.; Bickel, J.; Connick, R.; Connick, W.; Hosemann, P.

    2016-09-01

    A team of students from the University of California, Berkeley, participated in a blade-smithing competition hosted by the Minerals, Metals, and Materials Society at the TMS 2015 144th annual meeting and exhibition. Motivated by ancient forging methods, the UC Berkeley team chose to fabricate our blade from historical smithing techniques utilizing naturally-occurring deposits of iron ore. This approach resulted in receiving the "Best Example of a Traditional Blade Process/Ore Smelting Technique" award for our blade named "Berkelium." First, iron-enriched sand was collected from local beaches. Magnetite (Fe3O4) was then extracted from the sand and smelted into individual high- and low-carbon steel ingots. Layers of high- and low-carbon steels were forge-welded together, predominantly by hand, to form a composite material. Optical microscopy, energy dispersive spectroscopy, and Vickers hardness mechanical testing were conducted at different stages throughout the blade-making process to evaluate the microstructure and hardness evolution during formation. It was found that the pre-heat-treated blade microstructure was composed of ferrite and pearlite, and contained many nonmetallic inclusions. A final heat treatment was performed, which caused the average hardness of the blade edge to increase by more than a factor of two, indicating a martensitic transformation.

  14. Extraction studies of selected actinide ions from aqueous solutions with 4-benzoyl-2,4-dihydro-5-methyl-2-phenyl-3H-pyrazol-3-thione and tri-n-octylphosphine oxide

    Energy Technology Data Exchange (ETDEWEB)

    Hannink, N.J.; Hoffman, D.C. [Lawrence Berkeley Lab., CA (United States)]|[California Univ., Berkeley, CA (United States). Dept. of Chemistry; Smith, B.F. [Los Alamos National Lab., NM (United States)

    1991-11-01

    The first measurements of distribution coefficients (K{sub d}) for Cm(III), Bk(III), Cf(III), Es(III), and Fm(III) between aqueous perchlorate solutions and solutions of 4-benzoyl-2,4-dihydro-5-methyl-2-phenyl-3H-pyrazol-3-thione (BMPPT) and the synergist tri-n-octylphosphine oxide (TOPO) in toluene are reported. Curium-243, berkelium-250, californium-249, einsteinium-254, and fermium-253 were used in these studies. The K{sub d} for {sup 241}Am was also measured and is in agreement with previously published results. Our new results show that the K{sub d}`s decrease gradually with increasing atomic number for the actinides with a dip at Cf. In general, the K{sub d}`s for these actinides are about a factor of 5 to 10 greater than the K{sub d}`s for the homologous lanthanides at a pH of 2.9, a BMPPT concentration of 0.2 M, and a TOPO concentration of 0.04 M. The larger K{sub d}`s for the actinides are consistent with greater covalent bonding between the actinide metal ion and the sulfur bonding site in the ligand.

  15. Extraction studies of selected actinide ions from aqueous solutions with 4-benzoyl-2,4-dihydro-5-methyl-2-phenyl-3H-pyrazol-3-thione and tri-n-octylphosphine oxide

    Energy Technology Data Exchange (ETDEWEB)

    Hannink, N.J.; Hoffman, D.C. (Lawrence Berkeley Lab., CA (United States) California Univ., Berkeley, CA (United States). Dept. of Chemistry); Smith, B.F. (Los Alamos National Lab., NM (United States))

    1991-11-01

    The first measurements of distribution coefficients (K{sub d}) for Cm(III), Bk(III), Cf(III), Es(III), and Fm(III) between aqueous perchlorate solutions and solutions of 4-benzoyl-2,4-dihydro-5-methyl-2-phenyl-3H-pyrazol-3-thione (BMPPT) and the synergist tri-n-octylphosphine oxide (TOPO) in toluene are reported. Curium-243, berkelium-250, californium-249, einsteinium-254, and fermium-253 were used in these studies. The K{sub d} for {sup 241}Am was also measured and is in agreement with previously published results. Our new results show that the K{sub d}'s decrease gradually with increasing atomic number for the actinides with a dip at Cf. In general, the K{sub d}'s for these actinides are about a factor of 5 to 10 greater than the K{sub d}'s for the homologous lanthanides at a pH of 2.9, a BMPPT concentration of 0.2 M, and a TOPO concentration of 0.04 M. The larger K{sub d}'s for the actinides are consistent with greater covalent bonding between the actinide metal ion and the sulfur bonding site in the ligand.

  16. Extraction studies of selected actinide ions from aqueous solutions with 4-benzoyl-2,4-Dihydro-5-methyl-2-phenyl-3H-pyrazol-3-thione and Tri-n-octylphosphine oxide

    Energy Technology Data Exchange (ETDEWEB)

    Hannink, N.J.; Hoffman, D.C. [Lawrence Berkeley Lab., CA (United States); Smith, B.F. [Los Alamos National Lab., NM (United States)

    1992-07-01

    The first measurements of distribution coefficients (k{sub d}) for Cm(III), Bk(III), Cf(III), Es(III), and Fm(III) between aqueous perchlorate solutions and solutions of 4-benzoyl-2,4-dihydro-5-methyl-2-phenyl-3H-pyrazol-3-thione (BMPPT) and the synergist tri-n-octylphosphine oxide (TOPO) in toluene are reported. Curium-243, berkelium-250, californium-249, einsteinium-254, and fermium-253 were used in these studies. The K{sub d} for {sup 241}Am was also measured and is in agreement with previously published results. Our new results show that the K{sub d}`s decrease gradually with increasing atomic number for the actinides with a dip at Cf. In general, the K{sub d}`s for these actinides are about about a factor of 10 greater than the K{sub d}`s for the homologous lanthanides at a pH of 2.9, a BMPPT concentration of 0.2 M, and a TOPO concentration of 0.04 M. The larger K{sub d}`s for the actinides are consistent with greater covalent bonding between the actinide metal ion and the sulfur bonding site in the ligand. 9 refs., 2 figs., 1 tab.

  17. Characterization of a Viking Blade Fabricated by Traditional Forging Techniques

    Science.gov (United States)

    Vo, H.; Frazer, D.; Bailey, N.; Traylor, R.; Austin, J.; Pringle, J.; Bickel, J.; Connick, R.; Connick, W.; Hosemann, P.

    2016-12-01

    A team of students from the University of California, Berkeley, participated in a blade-smithing competition hosted by the Minerals, Metals, and Materials Society at the TMS 2015 144th annual meeting and exhibition. Motivated by ancient forging methods, the UC Berkeley team chose to fabricate our blade from historical smithing techniques utilizing naturally-occurring deposits of iron ore. This approach resulted in receiving the "Best Example of a Traditional Blade Process/Ore Smelting Technique" award for our blade named "Berkelium." First, iron-enriched sand was collected from local beaches. Magnetite (Fe3O4) was then extracted from the sand and smelted into individual high- and low-carbon steel ingots. Layers of high- and low-carbon steels were forge-welded together, predominantly by hand, to form a composite material. Optical microscopy, energy dispersive spectroscopy, and Vickers hardness mechanical testing were conducted at different stages throughout the blade-making process to evaluate the microstructure and hardness evolution during formation. It was found that the pre-heat-treated blade microstructure was composed of ferrite and pearlite, and contained many nonmetallic inclusions. A final heat treatment was performed, which caused the average hardness of the blade edge to increase by more than a factor of two, indicating a martensitic transformation.

  18. MiR-16 modulate temozolomide resistance by regulating BCL-2 in human glioma cells.

    Science.gov (United States)

    Han, Jing; Chen, Qianxue

    2015-01-01

    Temozolomide (TMZ) with radiotherapy is the current standard of care for newly diagnosed glioma. However, glioma patients who are treated with the drug often develop resistance to it and some other drugs. Recently studies have shown that microRNAs (miRNAs) play an important role in drug resistance. In present study, we first examined the sensitivity to temozolomide in six glioma cell lines, and established a resistant variant, U251MG/TR cells from TMZ-sensitive glioma cell line, U251MG. We then performed a comprehensive analysis of miRNA expressions in U251MG/TR and parental cells using cancer microRNA PCR Array. Among the downregulated microRNAs was miR-16, members of miR-15/16 family, whose expression was further validated by qRT-PCR in U251MG/TR and U251MG cells. The selective microRNA, miR-16 mimics or inhibitor was respectively transfected into U251MG/TR cells and AM38 cell. We found that treatment with the mimics of miR-16 greatly decreased the sensitivity of U251MG/TR cells to temozolomide, while sensitivity to these drugs was increased by treatment with the miR-16 inhibitor. In addition, the downregulation of miR-16 in temozolomide-sensitive AM38 cells was concurrent with the upregulation of Bcl-2 protein. Conversely, overexpression of miR-16 in temozolomide-resistant cells inhibited Bcl-2 expression and decreased temozolomide resistance. In conclusion, MiR-16 mediated temozolomide-resistance in glioma cells by modulation of apoptosis via targeting Bcl-2, which suggesting that miR-16 and Bcl-2 would be potential therapeutic targets for glioma therapy.

  19. Diet-dependent modulation of hippocampal expression of endocannabinoid signaling-related proteins in cannabinoid antagonist-treated obese rats.

    Science.gov (United States)

    Rivera, Patricia; Luque-Rojas, María Jesús; Pastor, Antoni; Blanco, Eduardo; Pavón, Francisco J; Serrano, Antonia; Crespillo, Ana; Vida, Margarita; Grondona, Jesús M; Cifuentes, Manuel; Bermúdez-Silva, Francisco J; de la Torre, Rafael; de Fonseca, Fernando Rodríguez; Suárez, Juan

    2013-01-01

    Diet-induced obesity produces changes in endocannabinoid signaling (ECS), influencing the regulation of energy homeostasis. Recently, we demonstrated that, in high-fat-diet-fed rats, blockade of CB1 receptor by AM251 not only reduced body weight but also increased adult neurogenesis in the hippocampus, suggesting an influence of diet on hippocampal cannabinoid function. To further explore the role of hippocampal ECS in high-fat-diet-induced obesity, we investigated whether the immunohistochemical expression of the enzymes that produce (diacylglycerol lipase alpha and N-acyl phosphatidylethanolamine phospholipase D) and degrade (monoacylglycerol lipase and fatty acid amino hydrolase) endocannabinoids may be altered in the hippocampus of AM251 (3 mg/kg)-treated rats fed three different diets: standard diet (normal chow), high-carbohydrate diet (70% carbohydrate) and high-fat diet (60% fat). Results indicated that AM251 reduced caloric intake and body weight gain, and induced a modulation of the expression of ECS-related proteins in the hippocampus of animals exposed to hypercaloric diets. These effects were differentially restricted to either the 2-arachinodoyl glycerol or anandamide signaling pathways, in a diet-dependent manner. AM251-treated rats fed the high-carbohydrate diet showed a reduction of the diacylglycerol lipase alpha : monoacylglycerol lipase ratio, whereas AM251-treated rats fed the high-fat diet showed a decrease of the N-acyl phosphatidylethanolamine phospholipase D : fatty acid amino hydrolase ratio. These results are consistent with the reduced levels of hippocampal endocannabinoids found after food restriction. Regarding the CB1 expression, AM251 induced specific changes focused in the CA1 stratum pyramidale of high-fat-diet-fed rats. These findings indicated that the cannabinoid antagonist AM251 modulates ECS-related proteins in the rat hippocampus in a diet-specific manner. Overall, these results suggest that the hippocampal ECS participates

  20. Synthetic Ligands of Cannabinoid Receptors Affect Dauer Formation in the Nematode Caenorhabditis elegans

    Science.gov (United States)

    Reis Rodrigues, Pedro; Kaul, Tiffany K.; Ho, Jo-Hao; Lucanic, Mark; Burkewitz, Kristopher; Mair, William B.; Held, Jason M.; Bohn, Laura M.; Gill, Matthew S.

    2016-01-01

    Under adverse environmental conditions the nematode Caenorhabditis elegans can enter an alternate developmental stage called the dauer larva. To identify lipophilic signaling molecules that influence this process, we screened a library of bioactive lipids and found that AM251, an antagonist of the human cannabinoid (CB) receptor, suppresses dauer entry in daf-2 insulin receptor mutants. AM251 acted synergistically with glucose supplementation indicating that the metabolic status of the animal influenced the activity of this compound. Similarly, loss of function mutations in the energy-sensing AMP-activated kinase subunit, aak-2, enhanced the dauer-suppressing effects of AM251, while constitutive activation of aak-2 in neurons was sufficient to inhibit AM251 activity. Chemical epistasis experiments indicated that AM251 acts via G-protein signaling and requires the TGF-β ligand DAF-7, the insulin peptides DAF-28 and INS-6, and a functional ASI neuron to promote reproductive growth. AM251 also required the presence of the SER-5 serotonin receptor, but in vitro experiments suggest that this may not be via a direct interaction. Interestingly, we found that other antagonists of mammalian CB receptors also suppress dauer entry, while the nonselective CB receptor agonist, O-2545, not only inhibited the activity of AM251, but also was able to promote dauer entry when administered alone. Since worms do not have obvious orthologs of CB receptors, the effects of synthetic CBs on neuroendocrine signaling in C. elegans are likely to be mediated via another, as yet unknown, receptor mechanism. However, we cannot exclude the existence of a noncanonical CB receptor in C. elegans. PMID:27172180

  1. Cell context-dependent dual effects of EFEMP1 stabilizes subpopulation equilibrium in responding to changes of in vivo growth environment.

    Science.gov (United States)

    Hu, Yuanjie; Ke, Chao; Ru, Ning; Chen, Yumay; Yu, Liping; Siegel, Eric R; Linskey, Mark E; Wang, Ping; Zhou, Yi-Hong

    2015-10-13

    Conflicting functions of EFEMP1 in cancer have been reported. Using two syngeneic glioma cell lines (U251 and U251-NS) carrying two different principal cell subpopulations that express high or low EGFR, and that are able to interconvert via mis-segregation of chromosome 7 (Chr7), we studied EFEMP1's cell-context-dependent functions in regulating subpopulation equilibrium, here defined by the percentage of cells carrying different copies of Chr7. We found that EFEMP1 attenuated levels of EGFR and cellular respiration in high-EGFR-expressing cells, but increased levels of NOTCH1, MMP2, cell invasiveness, and both oxidative phosphorylation and glycolytic respiration in low-EGFR-expressing cells. Consistently, EFEMP1 suppressed intracranial xenograft formation in U251 and promoted its formation in U251-NS. Interestingly, subpopulation equilibria in xenografts of U251-NS without EFEMP1 overexpression were responsive to inoculum size (1, 10 and 100 thousand cells), which may change the tumor-onset environment. It was not observed in xenografts of U251-NS with EFEMP1 overexpression. The anti-EGFR function of EFEMP1 suppressed acceleration of growth of U251-NS, but not the subpopulation equilibrium, when serially passed under a different (serum-containing adherent) culture condition. Overall, the data suggest that the orthotopic environment of the brain tumor supports EFEMP1 in carrying out both its anti-EGFR and pro-invasive/cancer stem cell-transforming functions in the two glioma cell subpopulations during formation of a single tumor, where EFEMP1 stabilizes the subpopulation equilibrium in response to alterations of the growth environment. This finding implies that EFEMP1 may restrain cancer plasticity in coping with ever-changing tumor microenvironments and/or therapeutic-intervention stresses.

  2. Effects of CB1 receptor agonism and antagonism on behavioral fear and physiological stress responses in adult intact, ovariectomized, and estradiol-replaced female rats.

    Science.gov (United States)

    Simone, J J; Malivoire, B L; McCormick, C M

    2015-10-15

    There is growing interest in the development of cannabis-based therapies for the treatment of fear and anxiety disorders. There are a few studies, but none in females, of the effects of the highly selective cannabinoid receptor type 1 (CB1) agonist, arachidonyl 2'-chlorethylamide (ACEA), on behavioral fear. In experiment 1 involving gonadally-intact females, ACEA (either 0.1 or 0.01 mg/kg) was without effect in the elevated plus maze (EPM), and the lower dose decreased anxiety in the open field test (OFT). AM251 increased anxiety in the EPM and decreased locomotor activity in the OFT. Twenty-four hours after fear conditioning, neither ACEA nor AM251 affected generalized fear or conditioned fear recall. AM251 and 0.1 mg/kg ACEA impaired, and 0.01 mg/kg ACEA enhanced, within-session fear extinction. AM251 increased plasma corticosterone concentrations after the fear extinction session, whereas ACEA was without effect. Based on evidence that estradiol may moderate the effects of CB1 receptor signaling in females, experiment 2 involved ovariectomized (OVX) rats provided with 10-μg 17β-Estradiol and compared with OVX rats without hormone replacement (oil vehicle). Irrespective of hormone treatment, AM251 increased anxiety in the EPM, whereas ACEA (0.01 mg/kg) was without effect. Neither hormone nor drug altered anxiety in the OFT, but estradiol increased and AM251 decreased distance traveled. After fear conditioning, AM251 decreased generalized fear. Neither hormone nor drug had any effect on recall or extinction of conditioned fear, however, ACEA and AM251 increased fear-induced plasma corticosterone concentrations. Further, when results with intact rats were compared with those from OVX rats, gonadal status did not moderate the effects of either AM251 or ACEA, although OVX displayed greater anxiety and fear than did intact rats. Thus, the effects of CB1 receptor antagonism and agonism in adult female rats do not depend on ovarian estradiol.

  3. Conditioned Media from Human Adipose Tissue-Derived Mesenchymal Stem Cells and Umbilical Cord-Derived Mesenchymal Stem Cells Efficiently Induced the Apoptosis and Differentiation in Human Glioma Cell Lines In Vitro

    Directory of Open Access Journals (Sweden)

    Chao Yang

    2014-01-01

    Full Text Available Human mesenchymal stem cells (MSCs have an intrinsic property for homing towards tumor sites and can be used as tumor-tropic vectors for tumor therapy. But very limited studies investigated the antitumor properties of MSCs themselves. In this study we investigated the antiglioma properties of two easily accessible MSCs, namely, human adipose tissue-derived mesenchymal stem cells (ASCs and umbilical cord-derived mesenchymal stem cells (UC-MSCs. We found (1 MSC conditioned media can significantly inhibit the growth of human U251 glioma cell line; (2 MSC conditioned media can significantly induce apoptosis in human U251 cell line; (3 real-time PCR experiments showed significant upregulation of apoptotic genes of both caspase-3 and caspase-9 and significant downregulation of antiapoptotic genes such as survivin and XIAP after MSC conditioned media induction in U 251 cells; (4 furthermore, MSCs conditioned media culture induced rapid and complete differentiation in U251 cells. These results indicate MSCs can efficiently induce both apoptosis and differentiation in U251 human glioma cell line. Whereas UC-MSCs are more efficient for apoptosis induction than ASCs, their capability of differentiation induction is not distinguishable from each other. Our findings suggest MSCs themselves have favorable antitumor characteristics and should be further explored in future glioma therapy.

  4. The cannabinoid system in the retrosplenial cortex modulates fear memory consolidation, reconsolidation, and extinction

    Science.gov (United States)

    Sachser, Ricardo Marcelo; Crestani, Ana Paula; Quillfeldt, Jorge Alberto; Mello e Souza, Tadeu

    2015-01-01

    Despite the fact that the cannabinoid receptor type 1 (CB1R) plays a pivotal role in emotional memory processing in different regions of the brain, its function in the retrosplenial cortex (RSC) remains unknown. Here, using contextual fear conditioning in rats, we showed that a post-training intra-RSC infusion of the CB1R antagonist AM251 impaired, and the agonist CP55940 improved, long-term memory consolidation. Additionally, a post-reactivation infusion of AM251 enhanced memory reconsolidation, while CP55940 had the opposite effect. Finally, AM251 blocked extinction, whereas CP55940 facilitated it and maintained memory extinguished over time. Altogether, our data strongly suggest that the cannabinoid system of the RSC modulates emotional memory. PMID:26572648

  5. Cross-categorization of legal concepts across boundaries of legal systems: in consideration of inferential links

    DEFF Research Database (Denmark)

    Glückstad, Fumiko Kano; Herlau, Tue; Schmidt, Mikkel Nørgaard

    2014-01-01

    This work contrasts Giovanni Sartor’s view of inferential semantics of legal concepts (Sartor in Artif Intell Law 17:217–251, 2009) with a probabilistic model of theory formation (Kemp et al. in Cognition 114:165–196, 2010). The work further explores possibilities of implementing Kemp’s probabili......This work contrasts Giovanni Sartor’s view of inferential semantics of legal concepts (Sartor in Artif Intell Law 17:217–251, 2009) with a probabilistic model of theory formation (Kemp et al. in Cognition 114:165–196, 2010). The work further explores possibilities of implementing Kemp...... to the International Standard Classification of Education. The main contribution of this work is the proposal of a conceptual framework of the cross-categorization approach that, inspired by Sartor (Artif Intell Law 17:217–251, 2009), attempts to explain reasoner’s inferential mechanisms....

  6. EdU及流式细胞术检测FRK对脑胶质瘤细胞增殖和凋亡的影响

    Institute of Scientific and Technical Information of China (English)

    闫浩平

    2013-01-01

    目的:研究FRK是否调控胶质瘤细胞的增殖与凋亡.方法应用EdU及流式细胞术检测FRK是否影响胶质瘤U251细胞增殖、凋亡.结果 EdU结果显示:过表达FRK后脑胶质瘤U251细胞增殖减少约60%,流式细胞仪检测发现过表达FRK后脑胶质瘤细胞U251细胞凋亡增多约120%.结论FRK可能调控脑胶质瘤细胞的增殖和凋亡.

  7. Interaction between orexin A and cannabinoid system in the lateral hypothalamus of rats and effects of subchronic intraperitoneal administration of cannabinoid receptor inverse agonist on food intake and the nutritive utilization of protein.

    Science.gov (United States)

    Merroun, I; El Mlili, N; Martinez, R; Porres, J M; Llopis, J; Ahabrach, H; Aranda, P; Sanchez Gonzalez, C; Errami, M; Lopez-Jurado, M

    2015-04-01

    Crosstalk may occur between cannabinoids and other systems controlling appetite, since cannabinoid receptors are present in hypothalamic circuits involved in feeding regulation, and likely to interact with orexin. In this study, an immunohistochemical approach was used to examine the effect of the intracerebroventricular administration of cannabinoid receptor inverse agonist AM 251 on orexin neuropeptide in the hypothalamic system. AM-activated neurons were identified using c-Fos as a marker of neuronal activity. The results obtained show that AM 251 decreases orexin A immunoreactivity, and that it increases c-Fos-immunoreactive neurons within the hypothalamus when compared with the vehicle-injected control group. We also studied the effects of subchronic intraperitoneal administration of AM 251 on food intake, body weight, and protein utilization. The administration of AM 251 at 1, 2, or 5 mg/kg led to a significant reduction in food intake, along with a significant decrease in the digestive utilization of protein in the groups injected with 1 and 2 mg/kg. There was a dose-related slowdown in weight gain, especially at the doses of 2 and 5 mg/kg, during the initial days of the trial. The absence of this effect in the pair-fed group reveals that any impairment to digestibility was the result of administering AM 251. These data support our conclusion that hypothalamic orexigenic neuropeptides are involved in the reduction of appetite and mediated by the cannabinoid receptor inverse agonist. Furthermore, the subchronic administration of AM 251, in addition to its effect on food intake, has significant effects on the digestive utilization of protein.

  8. Psr is involved in regulation of glucan production, and double deficiency of BrpA and Psr is lethal in Streptococcus mutans.

    Science.gov (United States)

    Bitoun, Jacob P; Liao, Sumei; McKey, Briggs A; Yao, Xin; Fan, Yuwei; Abranches, Jacqueline; Beatty, Wandy L; Wen, Zezhang T

    2013-03-01

    Streptococcus mutans, the primary causative agent of dental caries, contains two paralogues of the LytR-CpsA-Psr family proteins encoded by brpA and psr, respectively. Previous studies have shown that BrpA plays an important role in cell envelope biogenesis/homeostasis and affects stress responses and biofilm formation by Strep. mutans, traits critical to cariogenicity of this bacterium. In this study, a Psr-deficient mutant, TW251, was constructed. Characterization of TW251 showed that deficiency of Psr did not have any major impact on growth rate. However, when subjected to acid killing at pH 2.8, the survival rate of TW251 was decreased dramatically compared with the parent strain UA159. In addition, TW251 also displayed major defects in biofilm formation, especially during growth with sucrose. When compared to UA159, the biofilms of TW251 were mainly planar and devoid of extracellular glucans. Real-time-PCR and Western blot analyses revealed that deficiency of Psr significantly decreased the expression of glucosyltransferase C, a protein known to play a major role in biofilm formation by Strep. mutans. Transmission electron microscopy analysis showed that deficiency of BrpA caused alterations in cell envelope and cell division, and the most significant defects were observed in TW314, a Psr-deficient and BrpA-down mutant. No such effects were observed with Psr mutant TW251 under similar conditions. These results suggest that while there are similarities in functions between BrpA and Psr, distinctive differences also exist between these two paralogues. Like Bacillus subtilis but different from Staphylococcus aureus, a functional BrpA or Psr is required for viability in Strep. mutans.

  9. Chryseobacterium chengduensis sp. nov. isolated from the air of captive giant panda enclosures in Chengdu, China.

    Science.gov (United States)

    Wen, Cai-Fang; Xi, Li-Xin; Zhao, Shan; Hao, Zhong-Xiang; Luo, Lu; Liao, Hong; Chen, Zhen-Rong; She, Rong; Han, Guo-Quan; Cao, San-Jie; Wu, Rui; Yan, Qi-Gui; Hou, Rong

    2016-08-01

    A Gram-negative, aerobic, non-motile, rod-shaped bacterial strain, designated 25-1(T), was isolated from the air inside giant panda enclosures at the Chengdu Research Base of Giant Panda Breeding, China. Strain 25-1(T) grew optimally at pH 7.0-8.0, at 28-30 °C and in the presence of NaCl concentrations from 0.0% to 0.5 %. 16S rRNA gene sequence analysis indicated that strain 25-1(T) belongs to the genus Chryseobacterium within the family Flavobacteriaceae and is related most closely to C. carnis G81(T) (96.4% similarity), C. lathyri RBA2-6(T) (95.8% similarity), and C. zeae JM1085(T) (95.8% similarity). Its genomic DNA G+C molar composition was 36.2%. The major cellular fatty acids were iso-C15:0 (44.0%), iso-C17:0 3OH (19.8%) and C16:1 ω7c/16:1 ω6c (12.7%). The only isoprenoid quinone was menaquinone 6 (MK-6). The major polar lipids were phosphatidylethanolamine, two unidentified amino lipids and two unidentified lipids. The DNA-DNA relatedness between strain 25-1(T) and C. lathyri RBA2-6(T) was 38%. Phenotypic, genotypic, and phylogenetic characteristics indicated that strain 25-1(T) is a novel member of the genus Chryseobacterium, for which the name C. chengduensis sp. nov. is proposed. The type strain is 25-1(T) (CCTCC AB2015133(T)=DSM 100396(T)).

  10. Over-expression of tetraspanin 8 in malignant glioma regulates tumor cell progression

    Energy Technology Data Exchange (ETDEWEB)

    Pan, Si-Jian [Department of Neurosurgery, Rui Jin Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200025 (China); Wu, Yue-Bing [Department of Internal Medicine Oncology, Hubei Cancer Hospital, Wuhan, Hubei 430079 (China); Cai, Shang [Department of Radiotherapy and Oncology, the Second Affiliated Hospital of Soochow University, Suzhou 21500 (China); Pan, Yi-Xin; Liu, Wei [Department of Stereotactic and Functional Neurosurgery, Rui Jin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025 (China); Bian, Liu-Guan [Department of Neurosurgery, Rui Jin Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200025 (China); Sun, Bomin [Department of Stereotactic and Functional Neurosurgery, Rui Jin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025 (China); Sun, Qing-Fang, E-mail: sunqingfang11@163.com [Department of Neurosurgery, Rui Jin Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200025 (China)

    2015-03-13

    Tumor cell invasion and proliferation remain the overwhelming causes of death for malignant glioma patients. To establish effective therapeutic methods, new targets implied in these processes have to be identified. Tetraspanin 8 (Tspn8) forms complexes with a large variety of trans-membrane and/or cytosolic proteins to regulate several important cellular functions. In the current study, we found that Tspn8 was over-expressed in multiple clinical malignant glioma tissues, and its expression level correlated with the grade of tumors. Tspn8 expression in malignant glioma cells (U251MG and U87MG lines) is important for cell proliferation and migration. siRNA-mediated knockdown of Tspn8 markedly reduced in vitro proliferation and migration of U251MG and U87MG cells. Meanwhile, Tspn8 silencing also increased the sensitivity of temozolomide (TMZ), and significantly increased U251MG or U87MG cell death and apoptosis by TMZ were achieved with Tspn8 knockdown. We observed that Tspn8 formed a complex with activated focal adhesion kinase (FAK) in both human malignant glioma tissues and in above glioma cells. This complexation appeared required for FAK activation, since Tspn8 knockdown inhibited FAK activation in U251MG and U87MG cells. These results provide evidence that Tspn8 contributes to the pathogenesis of glioblastoma probably by promoting proliferation, migration and TMZ-resistance of glioma cells. Therefore, targeting Tspn8 may provide a potential therapeutic intervention for malignant glioma. - Highlights: • Tspn8 is over-expressed in multiple clinical malignant glioma tissues. • Tspn8 expression is correlated with the grade of malignant gliomas. • Tspn8 knockdown suppresses U251MG/U87MG proliferation and in vitro migration. • Tspn8 knockdown significantly increases TMZ sensitivity in U251MG/U87MG cells. • Tspn8 forms a complex with FAK, required for FAK activation.

  11. Participation of endocannabinoids in rapid suppression of stress responses by glucocorticoids in neonates.

    Science.gov (United States)

    Buwembo, A; Long, H; Walker, C-D

    2013-09-26

    In adult rodents, endocannabinoids (eCBs) regulate fast glucocorticoid (GC) feedback in the hypothalamus-pituitary adrenal (HPA) axis, acting as retrograde messengers that bind to cannabinoid receptors (CB1R) and inhibit glutamate release from presynaptic CRH neurons in the paraventricular nucleus of the hypothalamus (PVN). During the first two weeks of life, rat pups exhibit significant CRH and ACTH responses to stress although the adrenal GC output remains reduced. At the same time, pups also display increased sensitivity to GC feedback, but it is unclear whether eCBs play a role in mediating fast GC feedback in neonatal life. In our studies, we examined the role of eCBs in the rapid suppression of anoxia-induced ACTH release and determined whether eCB action could be modulated by the levels of circulating GCs present at the time of stress. PND8 pups were subjected to 3-min anoxia with AM251, a CB1R blocker, injected 30 min prior to stress onset. The effects of either metyrapone (MET) (a steroidogenic 11 beta-hydroxylase blocker) or methylprednisolone (PRED) (a synthetic GC) pretreatment on AM251 effect and the stress response were evaluated. Treatment with AM251 before stress onset tended to increase overall ACTH and CORT secretion, and also delayed the return to baseline ACTH. The AM251 effect on ACTH in PND8 pups was lost in MET-treated pups, who exhibited high basal and stimulated ACTH release and no CORT response to stress. Methylprednisolone suppressed ACTH stress responses although AM251 still delayed restoration of ACTH levels to the baseline. This suggests that the eCB effect on ACTH secretion in neonates is most evident when there is a dynamic fluctuation of corticosterone levels. Interestingly, AM251 increased basal and stimulated corticosterone secretion in all treatments including MET, suggestive of a direct action of CB1R blockade on adrenal steroidogenesis.

  12. 芯片—基因组杂交技术对胶质瘤细胞中与顺铂耐药相关分子标志物的筛选

    Institute of Scientific and Technical Information of China (English)

    张杨; 王成昆; 刘红光

    2016-01-01

    目的 筛选芯片—基因杂交技术对胶质瘤细胞中与顺铂耐药相关分子标志物.方法 运用芯片—基因组杂交技术来对顺铂耐药胶质瘤癌细胞进行相关分子的标志物进行筛选,通过对高耐顺铂的U251/CP2细胞和亲代细胞U251细胞进行筛选,从顺铂耐药的分子标志物入手进行分析研究,从已得结果中进行验证检验.结果 人补体因子H相关蛋白1的基因和人补体因子H相关蛋白3的基因片段在U251细胞中的DNA中均缺失了一个拷贝,而在U251/CP2细胞中出现了完全的却是,在进行实验的研究分析中,IL-7基因在U251/CP2细胞的基因拷贝基因数量是U251细胞的2倍以上.结论 经CFHR1/CFHR3和IL-7三个胶质基因均可能和胶质瘤细胞的抗药性有关,由于现有的设施仍存在一定的缺陷,本次实验仅供参考.

  13. MicroRNA-181b inhibits cellular proliferation and invasion of glioma cells via targeting Sal-like protein 4.

    Science.gov (United States)

    Zhou, Yu; Peng, Yong; Liu, Min; Jiang, Yugang

    2016-11-17

    MicroRNAs (miRs), a class of 18-25 nucleotides in length non-coding RNAs, are able to suppress gene expression by targeting complementary regions of mRNAs and inhibiting protein translation Recently, miR-181b was found to playa suppressive role in glioma, but the regulatory mechanism of miR-181b in the malignant phenotypes of glioma cells remains largely unclear. Here we found that miR-181b was significantly downregulated in glioma tissues when compared with normal brain tissues, and decreased miR-181b levels were significantly associated with high pathology grade and poor prognosis of patients with glioma. Moreover, miR-181b was also downregulated in glioma cell lines (U87, SHG44, U373, and U251) compared to normal astrocytes. Overexpression of miR-181b significantly decreased the proliferation, migration, and invasion of glioma U251 cells. Sal-like protein 4 (SALL4) was identified as a novel target gene of miR-181b in U251 cells. The expression of SALL4 was significantly upregulated in glioma tissues and cell lines, and an inverse correlation was observed between the miR-181b and SALL4 expression levels in glioma. Further investigation showed that the protein expression of SALL4 was negatively regulated by miR-181b in U251 cells. Knockdown of SALL4 significantly inhibited the proliferation, migration and invasion of U251 cells, while overexpression of SALL4 effectively reversed the suppressive effects of miR-181b on these malignant phenotypes of U251 cells. In conclusion, our study demonstrates that miR-181b has suppressive effects on the malignant phenotypes of glioma cells, partly at least, via directly targeting SALL4. Therefore, the miR-181b/SALL4 axis may become a potential therapeutic target for glioma.

  14. Prenatal Diagnosis of Cleft Palates Through Oral Fissure%经口裂超声扫查诊断胎儿腭裂的探讨

    Institute of Scientific and Technical Information of China (English)

    尹春林; 罗琼秀; 刘燕飞; 贺新兰; 黄金娟; 康秋香; 杨引洪

    2012-01-01

    Objective To study the two dimensional ultrasonic diagnosis of fetus cleft palates through the oral fissure. Methods Every fetus was scaned the hard palate and soft palate through oral fissure and submaxilla triangle except the detailed rountine ultrasound sacn. Results The display rates of hard palate and soft palate is 94. 82%(238/ 251) and 91. 63% (230/251)respectively by the sound beam through oral fissure, and the display rates of hard palate and soft palate is 13. 15%(33/251) ,14. 34% (36/251)respectively by the sound beam through submaxilla triangle. The results demonstrated that display rates of hard palate and soft palate be significantly higher by the sound beam through oral fissure than that through submaxilla triangle. Conclusions The examination of cleft palate through the fetal oral fissure can improve the display rates of the hard palate and soft palate, and provide definitive diagnosis and degree of cleft palate.%目的 探讨二维超声经胎儿口裂扫查诊断腭裂的价值.方法 对胎儿完成超声系统检查后,分别经胎儿口裂及颌下三角扫查硬腭及软腭.结果 经口裂扫查法胎儿硬腭显示率94.82% (238/251)、软腭显示率91.63% (230/251).经颌下三角扫查法胎儿硬腭显示率13.15% (33/251)、软腭显示率14.34% (36/251).结果显示经口裂扫查法对软硬腭的显示率显著高于经颌下三角扫查法.结论 经口裂扫查法能提高硬腭及软腭的显示率,明确有无腭裂及其程度,能提高腭裂的产前检出率.

  15. 人钠/碘同向转运体基因转染胶质瘤细胞介导放射性碘治疗的研究%Transfer of the sodium/iodide symporter gene into gliomas for radioiodine therapy in vitro

    Institute of Scientific and Technical Information of China (English)

    谭建; 李玮; 刘晓华; 肖茜; 贾强; 李宁

    2008-01-01

    Objective The most frequent brain tumors are the gliomas.Glioblastomas arc largely incurable secondary to their rapid,aggressive and diffusely infiltrative growth pattern and hypervascularity.This study,aimed at investigating the possibility of transecting human sodium/iodide symporter(hNIS)gene into human glioma cell lines to facilitate radioactive iodide treatment in vitro.Methods Transecting hNIS gene into human glioma cell lines U251 was performed by recombinant expression plasmids with lipofectamine 2000-plasmid complexes.The hNIS gene cell lines with stable expression(hNIS-U251)were selected through G418 antibiotic constraint.The hNIS-U251 gene cell lines were then evaluated for their biologic functions,including 125I uptake assay.125 I influx-course.125I-efflux-course.131I inhibitory effect on cellular proliferation by 3-(4,5-dimethylthiazol-2-y1)-2,5-diphenylte-trazolium bromide(MTT)assay and flow cytometer.Results We were successful in transecting hNIS gene into human glioma cell lines by recombinant expression plasmids.and were able to obtain hNIS gene cell lines(hNIS-U251)with stable expression.The hNIS-U251 cell lines could intake and bind radioactive iodide by hNIS gene.The uptakes of125 Iwere 117 fold higher in hNIS-U251 cell lines than U251 cell lines[(50 469.88±997.29),(432.92±89.28)counts·min-1,respectively ].And the proliferation index of hNIS-U251 cell lines was lower than U251 cell lines after incubating with 131I.Conclusion The hNIS gene with stable gene expression (hNIS-U251)cell lines could be labeled by 131I with a high efficiency,thereby may function effectively in the treatment of glioma-related brain tumors.%目的 在体外细胞实验中证明转染人钠/碘同向转运体(hNIS)基因介导放射性碘治疗胶质瘤是有效的.方法 以脂质体转染法、用重组质粒将hNIS基因转染至人胶质瘤细胞株U251中.经过G418硫酸盐筛选获得稳定表达hNIS的细胞株(hNIS-U251),然后进行体外摄125I

  16. Polymorphism of inflammatory genes and arsenic methylation capacity are associated with urothelial carcinoma

    Energy Technology Data Exchange (ETDEWEB)

    Wu, Chia-Chang [School of Public Health, College of Public Health and Nutrition, Taipei Medical University, Taipei, Taiwan (China); Department of Urology, Taipei Medical University—Shuang Ho Hospital, Taipei, Taiwan (China); Huang, Yung-Kai [School of Oral Hygiene, College of Oral Medicine, Taipei Medical University, Taipei, Taiwan (China); Chung, Chi-Jung [Department of Health Risk Management, College of Public Health, China Medical University and Hospital, Taichung, Taiwan (China); Department of Medical Research, China Medical University Hospital, Taichung, Taiwan (China); Huang, Chao-Yuan; Pu, Yeong-Shiau [Department of Urology, National Taiwan University Hospital, College of Medicine National Taiwan University, Taipei, Taiwan (China); Shiue, Horng-Sheng [Department of Chinese Medicine, Chang Gung Memorial Hospital, Taipei, Taiwan (China); Lai, Li-An [School of Public Health, College of Public Health and Nutrition, Taipei Medical University, Taipei, Taiwan (China); Lin, Ying-Chin [Department of Family Medicine, Shung Ho Hospital, Taipei Medical University, Taipei, Taiwan (China); Department of Health Examination, Wan Fang Hospital, Taipei Medical University, Taipei, Taiwan (China); Su, Chien-Tien [Department of Family Medicine, Taipei Medical University Hospital, Taipei, Taiwan (China); Hsueh, Yu-Mei, E-mail: ymhsueh@tmu.edu.tw [School of Public Health, College of Public Health and Nutrition, Taipei Medical University, Taipei, Taiwan (China); Department of Public Health, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan (China)

    2013-10-01

    Chronic exposure to arsenic can generate reactive oxidative species, which can induce certain proinflammatory cytokines such as tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6) and interleukin-8 (IL-8). TNF-α, IL-6 and IL-8 have been shown to be involved in the development and progression of various cancers, including bladder cancer. This study aimed to investigate the joint effect of the polymorphism of TNF-α − 308 G/A, IL-6 − 174 G/C, IL-8 − 251 T/A and urinary arsenic profiles on urothelial carcinoma (UC) risk. This study evaluated 300 pathologically-confirmed cases of UC and 594 cancer-free controls. Urinary arsenic species were detected using high-performance liquid chromatography-linked hydride generator and atomic absorption spectrometry. The polymorphism of TNF-α − 308 G/A, IL-6 − 174 G/C and IL-8 − 251 T/A was determined using polymerase chain reaction-restriction fragment length polymorphism. The joint effects on UC risk were estimated by odds ratios and 95% confidence intervals using unconditional logistic regression. We found that the TNF-α − 308 A/A and IL-8 − 251 T/T polymorphisms were significantly associated with UC. Moreover, significant dose–response joint effect of TNF-α − 308 A/A or IL-8 − 251 T/T genotypes and arsenic methylation indices were seen to affect UC risk. The present results also showed a significant increase in UC risk in subjects with the IL-8 − 251 T/T genotype for each SD increase in urinary total arsenic and MMA%. In contrast, a significant decrease in UC risk was found in subjects who carried the IL-8 − 251 T/T genotype for each SD increase in DMA%. - Highlights: • Joint effect of the TNF-α -308 A/A genotype and urinary total arsenic affected UC. • Joint effect of the IL-8 -251 T/T genotype and urinary total arsenic affected UC. • Urinary total arsenic level, TNF-α -308 A/A and IL-8 -251 T/T genotype affected UC.

  17. Effective Behavior of Composite Materials.

    Science.gov (United States)

    2014-09-26

    7AD-A158 941 EFFECTIVE BEHAVIOR OF COMPOSITE MTERIRLS(A) NEW YORK i/i UNIV MY COURANT INST OF ATHEMATICAL SCIENCES 6CPAPANICOLAOU 23 APR 85 5274192... Courant ilfapphcabt e Instit.te of Math. Sciences AF0SR/NM 6c. ADDRESS Cit). State and ZIP Code, 7b. ADDRESS (City. State and ZIP Code) 251 Mercer St Bldg...Papanicolaou Courant Institute 251 Mercer Street New York, N.Y. 10012 i~istr~utlo2 During this period two thesis ipja b&have completed ’their work and have

  18. HCMV Infection Depress NGF Expression in Human Glioma Cells

    Institute of Scientific and Technical Information of China (English)

    Hai-tao WANG; Bin WANG; Zhi-jun LIU; Zhi-qiang BAI; Ling LI; Dong-meng QIAN; Zhi-yong YAN; Xu-xia SONG

    2009-01-01

    Human cytomegalovirus (HCMV) is the most common cause of congenital infection, resulting in birth defects such as microcephaly. In this study, RT-PCR and Western Blotting were performed to quantify the regulation of endogenic nerve growth factor expression in neuroglia cells by HCMV infection. The results showed that basal, endogenous NGF expression in U251 was unchanged during early HCMV infection. NGF expression is strongly down-regulated during the latent phase of infection. These results suggest that HCMV can depress the NGF expression in U251 cells.

  19. Radiochemical studies of neutron deficient actinide isotopes

    Energy Technology Data Exchange (ETDEWEB)

    Williams, K.E.

    1978-04-01

    The production of neutron deficient actinide isotopes in heavy ion reactions was studied using alpha, gamma, x-ray, and spontaneous fission detection systems. A new isotope of berkelium, /sup 242/Bk, was produced with a cross-section of approximately 10 ..mu..b in reactions of boron on uranium and nitrogen on thorium. It decays by electron capture with a half-life of 7.0 +- 1.3 minutes. The alpha-branching ratio for this isotope is less than 1% and the spontaneous fission ratio is less than 0.03%. Studies of (Heavy Ion, pxn) and (Heavy Ion, ..cap alpha..xn) transfer reactions in comparison with (Heavy ion, xn) compound nucleus reactions revealed transfer reaction cross-sections equal to or greater than the compound nucleus yields. The data show that in some cases the yield of an isotope produced via a (H.I.,pxn) or (H.I.,..cap alpha..xn) reaction may be higher than its production via an xn compound nucleus reaction. These results have dire consequences for proponents of the ''Z/sub 1/ + Z/sub 2/ = Z/sub 1+2/'' philosophy. It is no longer acceptable to assume that (H.I.,pxn) and (H.I.,..cap alpha..xn) product yields are of no consequence when studying compound nucleus reactions. No evidence for spontaneous fission decay of /sup 228/Pu, /sup 230/Pu, /sup 232/Cm, or /sup 238/Cf was observed indicating that strictly empirical extrapolations of spontaneous fission half-life data is inadequate for predictions of half-lives for unknown neutron deficient actinide isotopes.

  20. Paul Scherrer Institute Scientific Report 1999. Volume I: Particles and Matter

    Energy Technology Data Exchange (ETDEWEB)

    Gobrecht, J.; Gaeggeler, H.; Herlach, D.; Junker, K.; Kettle, P.-R.; Kubik, P.; Zehnder, A. [eds.

    2000-07-01

    lthough originally planned for fundamental research in nuclear physics, the particle beams of pions, muons, protons and neutrons are now used in a large variety of disciplines in both natural science and medicine. The beams at PSI have the world's highest intensities and therefore allow certain experiments to be performed, which would not be possible elsewhere. The highlight of research this year was the first-ever determination of the chemical properties of the superheavy element {sup 107} Bohrium. This was undertaken, by an international team led by H. Gaeggeler of PSI's Laboratory for Radiochemistry. Bohrium was produced by bombarding a Berkelium target with Neon ions from the Injector I cyclotron and six atoms were detected after having passed through an online gas chromatography device. At the Laboratory for Particle Physics the focus has shifted from nuclear physics to elementary particle physics with about a fifty-fifty split between investigations of rare processes or particle decays using the high intensity muon, pion and recently also polarized neutron beams of PSI, and research at the highest energy frontier at CERN (Geneva) and DESY (Hamburg). Important space instrumentation has been contributed by the Laboratory for Astrophysics to the European Space Agency and NASA satellite programmes. The Laboratory for Micro and Nanotechnology continued to focus on research into molecular nanotechnology and SiGeC nanostructures, the latter with the aim of producing silicon based optoelectronics. Progress in 1999 in these topical areas is described in this report. A list of scientific publications in 1999 is also provided.

  1. Transuranium Processing Plant semiannual report of production, status, and plans for period ending December 31, 1975

    Energy Technology Data Exchange (ETDEWEB)

    King, L.J.; Bigelow, J.E.; Collins, E.D.

    1976-10-01

    Between July 1, 1975, and December 31, 1975, maintenance was conducted at TRU for a period of three months, 295 g of curium oxide (enough for approximately 26 HFIR targets) were prepared, 100 mg of high-purity /sup 248/Cm, were separated from /sup 252/Cf that had been purified during earlier periods, 11 HFIR targets were fabricated, and 28 product shipments were made. No changes were made in the chemical processing flowsheets normally used at TRU during this report period. However, three equipment racks were replaced (with two new racks) during this time. In Cubicle 6, the equipment replaced was that used to decontaminate the transplutonium elements from rare earth fission products and to separate curium from the heavier elements by means of the LiCl-based anion-exchange process. In Cubicle 5, the equipment used to separate the transcurium elements by high-pressure ion exchange and to purify berkelium by batch solvent extraction was replaced. Two neutron sources were fabricated, bringing the total fabricated to 79. One source that had been used in a completed project was returned to the TRU inventory and is available for reissue. Three sources, for which no further use was foreseen, were processed to isolate and recover the ingrown /sup 248/Cm and the residual /sup 252/Cf. Eight pellets, each containing 100 ..mu..g of high-purity /sup 248/Cm were prepared for irradiation in HFIR to study the production of /sup 250/Cm. The values currently being used for transuranium element decay data and for cross-section data in planning irradiation-processing cycles, calculating production forecasts, and assaying products are tabulated.

  2. SwissProt search result: AK107538 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK107538 002-129-H06 (Q9Y251) Heparanase precursor (EC 3.2.-.-) (Heparanase-1) (Hpa...1) (Endo-glucoronidase) [Contains: Heparanase 8 kDa subunit; Heparanase 50 kDa subunit] HPSE_HUMAN 2e-27 ...

  3. SwissProt search result: AK105099 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK105099 001-100-C07 (Q9Y251) Heparanase precursor (EC 3.2.-.-) (Heparanase-1) (Hpa...1) (Endo-glucoronidase) [Contains: Heparanase 8 kDa subunit; Heparanase 50 kDa subunit] HPSE_HUMAN 4e-11 ...

  4. SwissProt search result: AK072279 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK072279 J023018A16 (Q9Y251) Heparanase precursor (EC 3.2.-.-) (Heparanase-1) (Hpa1...) (Endo-glucoronidase) [Contains: Heparanase 8 kDa subunit; Heparanase 50 kDa subunit] HPSE_HUMAN 1e-24 ...

  5. SwissProt search result: AK072765 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK072765 J023139K11 (Q9Y251) Heparanase precursor (EC 3.2.-.-) (Heparanase-1) (Hpa1...) (Endo-glucoronidase) [Contains: Heparanase 8 kDa subunit; Heparanase 50 kDa subunit] HPSE_HUMAN 1e-24 ...

  6. SwissProt search result: AK060767 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK060767 001-033-A11 (Q9Y251) Heparanase precursor (EC 3.2.-.-) (Heparanase-1) (Hpa...1) (Endo-glucoronidase) [Contains: Heparanase 8 kDa subunit; Heparanase 50 kDa subunit] HPSE_HUMAN 4e-26 ...

  7. SwissProt search result: AK098886 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK098886 J013001J13 (Q9Y251) Heparanase precursor (EC 3.2.-.-) (Heparanase-1) (Hpa1...) (Endo-glucoronidase) [Contains: Heparanase 8 kDa subunit; Heparanase 50 kDa subunit] HPSE_HUMAN 4e-26 ...

  8. SwissProt search result: AK065263 [KOME

    Lifescience Database Archive (English)

    Full Text Available AK065263 J013002K06 (Q9Y251) Heparanase precursor (EC 3.2.-.-) (Heparanase-1) (Hpa1...) (Endo-glucoronidase) [Contains: Heparanase 8 kDa subunit; Heparanase 50 kDa subunit] HPSE_HUMAN 3e-30 ...

  9. Gclust Server: 116316 [Gclust Server

    Lifescience Database Archive (English)

    Full Text Available 116316 TET_937.m00002 Cluster Sequences Related Sequences(37) 251 Transposable elem...ent Tc3 transposase.-related chr_0_8254328_937; no annotation 1 1.00e-22 0.0 11.11 0.0 0.0 0.0 0.0 Show 116316 Cluster ID 116316

  10. METALS DEPOSITS

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    <正>20070291 Gong Ping (Northern Fujian Geological Party, Shaozou 354000) Discussion on Geological Characteristics and Control Factors of the Shimen Au-polymetallic Deposit in Zhenghe County, Fujian Province (Geology of Fujian, ISSN1001-3970, CN38-1080/P, 25(1), 2006, p.18-24, 2 illus., 2 tables, 1 ref.) Key words: gold deposits, polymetallic deposits, Fujian Province

  11. Dicty_cDB: Contig-U02747-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available .002 1 ( CV511896 ) kc49d02.y1 Xiphinema index CSEQDA01 Xiphinema ind... 54 0.006 1 ( CV511063 ) kc66f06.y1 Xiphinema... index CSEQDL01 Xiphinema ind... 54 0.006 1 ( CD179525 ) MS1-0016T-M251-E06-U.G MS1-0016 Schistosom

  12. Data of evolutionary structure change: 1BOQA-2QA9E [Confc[Archive

    Lifescience Database Archive (English)

    Full Text Available 1BOQA-2QA9E 1BOQ 2QA9 A E ANIVGGIEYSINNASLCSVGFSVTRGATKGFVTAGHCGT...VNATARIG---GAVVGTFAARVFPGNDRAWVSLT-SAQTLLPRVANGSSFVTVRGSTEAAVGAAVCRSGRTTGYQCGTITAKNITANYAE-GAVRGLTQGNACMGRGDSGGSWITSAGQA...indel> 0 1BOQ A 1BOQA...ine>VAL CA 251 VAL CA 326 GLY CA 354 2QA...9 E 2QA9E

  13. 76 FR 77382 - Airworthiness Directives; Continental Motors, Inc. (CMI) Reciprocating Engines

    Science.gov (United States)

    2011-12-13

    ... identified in this AD, contact Continental Motors, Inc., P.O. Box 90, Mobile, AL 36601; phone: (251) 438-3411... service information referenced in this AD, contact: Continental Motors, Inc., PO Box 90, Mobile, AL 36601... in Burlington, Massachusetts, on December 5, 2011. Peter A. White, Manager, Engine &...

  14. AcEST: BP918214 [AcEST

    Lifescience Database Archive (English)

    Full Text Available methyltransferase 2 OS=Ca... 31 4.9 sp|Q21771|CPG3_CAEEL Chondroitin proteoglycan 3 OS=Caenorhabditi... 30 ...Q Sbjct: 251 GATNAFATKMLYHFQHLRSPIQCVETYPTEKLQLQ 285 >sp|Q21771|CPG3_CAEEL Chondroitin proteoglycan 3 OS=Cae

  15. AcEST: DK953082 [AcEST

    Lifescience Database Archive (English)

    Full Text Available |GSE1_MOUSE Genetic suppressor element 1 OS=Mus musculu... 31 3.9 sp|Q70JA7|CHSS3_HUMAN Chondroitin sulfate ...98 QVEKARQSRLSSS*WYRV 251 Q K + LS+ ++V Sbjct: 1038 QKHKGNSALLSAEQSHKV 1055 >sp|Q70JA7|CHSS3_HUMAN Chondroitin

  16. An Empirical Investigation of Student Acceptance of Synchronous E-Learning in an Online University

    Science.gov (United States)

    Kang, Minseok; Shin, Won sug

    2015-01-01

    This study proposes an extended technology acceptance model to predict acceptance of synchronous e-learning by examining relationships among variables associated with factors influencing the technology acceptance of synchronous e-learning. Learners at an online university participated through an online survey; there were 251 respondents in all.…

  17. 76 FR 6161 - Annual Determination of Average Cost of Incarceration

    Science.gov (United States)

    2011-02-03

    ... No: 2011-2363] DEPARTMENT OF JUSTICE Bureau of Prisons Annual Determination of Average Cost of Incarceration AGENCY: Bureau of Prisons, Justice. ACTION: Notice. SUMMARY: The fee to cover the average cost of incarceration for Federal inmates in Fiscal Year 2009 was $25,251. The average annual cost to confine an...

  18. Data of evolutionary structure change: 1AIJS-2UX4L [Confc[Archive

    Lifescience Database Archive (English)

    Full Text Available 1AIJS-2UX4L 1AIJ 2UX4 S L YQNIFSQVQVRGPADLGMTEDVNLANRSGVGPFSTLLGW...nt> 0 1AIJ S 1AIJ...confEVID> 1 1AIJ S 1AIJ...LEU CA 260 ALA CA 251 LYS CA 279 1AIJ... S 1AIJS YGLSFAAPLKE

  19. 8 CFR 1280.6 - Bond to obtain clearance; form.

    Science.gov (United States)

    2010-01-01

    ... 8 Aliens and Nationality 1 2010-01-01 2010-01-01 false Bond to obtain clearance; form. 1280.6... IMMIGRATION REGULATIONS IMPOSITION AND COLLECTION OF FINES § 1280.6 Bond to obtain clearance; form. A bond to obtain clearance of a vessel or aircraft under section 231, 237, 239, 243, 251, 253, 254, 255, 256,...

  20. Stage-specific survival of epithelial cancers in North-Holland/Flevoland, The Netherlands

    NARCIS (Netherlands)

    Visser, O.; Leeuwen, van F.E.

    2005-01-01

    While stage is the most important factor for determining cancer survival, population-based survival data according to stage are rarely presented. We present such data for a large population diagnosed with cancer in the area covered by the Amsterdam Cancer Registry for the period 1989-2001 (n=108,251

  1. Exploring Motivational and Learning Differences with Mature Students in Post-Secondary Education

    Science.gov (United States)

    Murray, William C.; Smith, Leslie; Nielson, Keith

    2010-01-01

    This study examines the differences that exist among various age cohorts attending a post-secondary educational institution. Respondents were from the New Brunswick Community College's six separate campuses (n = 251). Significant differences were found to exist predominantly around social and family issues. Although differences did appear based on…

  2. 78 FR 61208 - Endangered and Threatened Wildlife and Plants; Listing the Blue-throated Macaw

    Science.gov (United States)

    2013-10-03

    ... International (BLI) and the Integrated Taxonomic Information System (ITIS) recognize the blue-throated macaw as...-throated macaws; it also has local, commercial, and ecosystem importance (Kyle 2005, p. 3; Moraes et al... systems and subsequent death (Gaskin 1989, pp. 249, 251, 252). Recently, an examination of tissue...

  3. Facebook Use and the Tendency to Ruminate among College Students: Testing Mediational Hypotheses

    Science.gov (United States)

    Locatelli, Sara M.; Kluwe, Katharina; Bryant, Fred B.

    2012-01-01

    Studies have found that general use of Facebook influences subjective well-being. However, fewer studies have explored the impact of specific use behaviors, such as information posted in status updates. The current study uses data collected from 251 Facebook-using undergraduate students through an online survey, and examines the valence and…

  4. Health-Related Quality of Life in Children Attending Special and Typical Education Greek Schools

    Science.gov (United States)

    Papadopoulou, D.; Malliou, P.; Kofotolis, N.; Vlachopoulos, S. P.; Kellis, E.

    2017-01-01

    The purpose of this study was to examine parental perceptions about Health Related Quality of Life (HRQoL) of typical education and special education students in Greece. The Pediatric Quality of Life Inventory (PedsQL) was administered to the parents of 251 children from typical schools, 46 students attending integration classes (IC) within a…

  5. Learning Style of Students and Practitioners in Five Health Professions.

    Science.gov (United States)

    Katz, Noomi; Heimann, Nanci

    1991-01-01

    An Israeli sample of 378 students and 251 practitioners in occupational therapy (OT), social work, nursing, physical therapy, and clinical psychology completed Kolb's Learning Style Inventory. Findings suggest greater variance in learning style among students. OT students were least abstract. Both OT students and practitioners were predominantly…

  6. Trans-ancestry genome-wide association study identifies 12 genetic loci influencing blood pressure and implicates a role for DNA methylation

    NARCIS (Netherlands)

    N. Kato (Norihiro); M. Loh (Marie); F. Takeuchi (Fumihiko); N. Verweij (Niek); X. Wang (Xu); W. Zhang (Weihua); T. NKelly (Tanika); D. Saleheen; B. Lehne (Benjamin); I.M. Leach (Irene Mateo); A. Drong (Alexander); J. Abbott (James); S. Wahl (Simone); S.-T. Tan (Sian-Tsung); W.R. Scott (William R.); G. Campanella (Gianluca); M. Chadeau-Hyam (Marc); U. Afzal (Uzma); T.S. Ahluwalia (Tarunveer Singh); M.J. Bonder (Marc Jan); P. Chen (Ping); A. Dehghan (Abbas); T.L. Edwards (Todd L.); T. Esko (Tõnu); M.J. Go (Min Jin); S.E. Harris (Sarah); J. Hartiala (Jaana); S. Kasela (Silva); A. Kasturiratne (Anuradhani); C.C. Khor; M.E. Kleber (Marcus); H. Li (Huaixing); Z.Y. Mok (Zuan Yu); M. Nakatochi (Masahiro); N.S. Sapari (Nur Sabrina); R. Saxena (Richa); A.F. Stewart (Alexandre F.); L. Stolk (Lisette); Y. Tabara (Yasuharu); A.L. Teh (Ai Ling); Y. Wu (Ying); J.-Y. Wu (Jer-Yuarn); Y. Zhang (Yi); I. Aits (Imke); A. Da Silva Couto Alves (Alexessander); S. Das; R. Dorajoo (Rajkumar); J. CHopewell (Jemma); Y.K. Kim (Yun Kyoung); R. WKoivula (Robert); J. Luan (Jian'An); L.-P. Lyytikäinen (Leo-Pekka); Q. NNguyen (Quang); M.A. Pereira (Mark A); D. Postmus (Douwe); O. TRaitakari (Olli); M. Scannell Bryan (Molly); R.A. Scott (Robert); R. Sorice; V. Tragante (Vinicius); M. Traglia (Michela); J. White (Jon); K. Yamamoto (Ken); Y. Zhang (Yonghong); L.S. Adair (Linda); A. Ahmed (Alauddin); K. Akiyama (Koichi); R. Asif (Rasheed); T. Aung (Tin); I. Barroso (Inês); A. Bjonnes (Andrew); T.R. Braun (Timothy R.); H. Cai (Hui); L.-C. Chang (Li-Ching); C.-H. Chen; C-Y. Cheng (Ching-Yu); Y.-S. Chong (Yap-Seng); F.S. Collins (Francis); R. Courtney (Regina); G. Davies (Gail); G. Delgado; L.D. Do (Loi D.); P.A. Doevendans (Pieter); R.T. Gansevoort (Ron); Y. Gao; T.B. Grammer (Tanja B); N. Grarup (Niels); J. Grewal (Jagvir); D. Gu (D.); G. SWander (Gurpreet); A.L. Hartikainen; S.L. Hazen (Stanley); J. He (Jing); C.K. Heng (Chew-Kiat); E.J.A. Hixso (E. James Ames); A. Hofman (Albert); C. Hsu (Chris); W. Huang (Wei); L.L.N. Husemoen (Lise Lotte); J.-Y. Hwang (Joo-Yeon); S. Ichihara (Sahoko); M. Igase (Michiya); M. Isono (Masato); J.M. Justesen (Johanne M.); T. Katsuya (Tomohiro); M. GKibriya (Muhammad); Y.J. Kim; M. Kishimoto (Miyako); W.-P. Koh (Woon-Puay); K. Kohara (Katsuhiko); M. Kumari (Meena); K. Kwek (Kenneth); N.R. Lee (Nanette); J. Lee (Jeannette); J. Liao (Jie); W. Lieb (Wolfgang); D.C. Liewald (David C.); T. Matsubara (Tatsuaki); Y. Matsushita (Yumi); T. Meitinger (Thomas); E. Mihailov (Evelin); L. Milani (Lili); R. Mills (Rebecca); K. Mononen (Kari); M. Müller-Nurasyid (Martina); T. Nabika (Toru); E. Nakashima (Eitaro); H.K. Ng (Hong Kiat); K. Nikus (Kjell); T. Nutile; T. Ohkubo (Takayoshi); K. Ohnaka (Keizo); S. Parish (Sarah); L. Paternoster (Lavinia); H. Peng (Hao); A. Peters (Annette); S. TPham (Son); M.J. Pinidiyapathirage (Mohitha J.); M. Rahman (Mahfuzar); H. Rakugi (Hiromi); O. Rolandsson (Olov); M.A. Rozario (Michelle Ann); D. Ruggiero; C. Sala (Cinzia); R. Sarju (Ralhan); K. Shimokawa (Kazuro); H. Snieder (Harold); T. Sparsø (Thomas); W. Spiering (Wilko); J.M. Starr (John); D.J. Stott (David J.); D. OStram (Daniel); T. Sugiyama (Takao); S. Szymczak (Silke); W.H.W. Tang (W.H. Wilson); L. Tong (Lin); S. Trompet (Stella); V. Turjanmaa (Väinö); H. Ueshima (Hirotsugu); A.G. Uitterlinden (André); S. Umemura (Satoshi); M. Vaarasmaki (Marja); R.M. Dam (Rob Mvan); W.H. van Gilst (Wiek); D.J. van Veldhuisen (Dirk); J. Viikari (Jorma); M. Waldenberger (Melanie); Y. Wang (Yiqin); A. Wang (Aili); R. Wilson (Rory); T.-Y. Wong (Tien-Yin); Y.-B. Xiang (Yong-Bing); S. Yamaguchi (Shuhei); X. Ye (Xingwang); R. Young (Robin); T.L. Young (Terri); J.-M. Yuan (Jian-Min); X. Zhou (Xueya); F.W. Asselbergs (Folkert); M. Ciullo; R. Clarke (Robert); P. Deloukas (Panagiotis); A. Franke (Andre); W.F. Paul (W. Frank); S. Franks (Steve); Y. Friedlander (Yechiel); M.D. Gross (Myron D.); Z. Guo (Zhirong); T. Hansen (T.); M.-R. Jarvelin (Marjo-Riitta); T. Jørgensen (Torben); J.W. Jukema (Jan Wouter); M. Kähönen (Mika); H. Kajio (Hiroshi); M. Kivimaki (Mika); J.-Y. Lee (Jong-Young); T. Lehtimäki (Terho); A. Linneberg (Allan); T. Miki (Tetsuro); O. Pedersen (Oluf); N.J. Samani (Nilesh); T.I.A. Sørensen (Thorkild); R. Takayanagi (Ryoichi); D. Toniolo (Daniela); H. Ahsan (Habibul); H. Allayee (Hooman); Y.-T. Chen (Yuan-Tsong); J. Danesh (John); I.J. Deary (Ian J.); O.H. Franco (Oscar); L. Franke (Lude); B. THeijman (Bastiaan); J.D. Holbrook (Joanna D.); A.J. Isaacs (Aaron); B.-J. Kim (Bong-Jo); X. Lin (Xu); J. Liu (Jianjun); W. März (Winfried); A. Metspalu (Andres); K.L. Mohlke (Karen); K. Sangher; D. Harambir (Dharambir); X.-O. Shu (Xiao-Ou); J.B.J. van Meurs (Joyce); E.N. Vithana (Eranga); A.R. Wickremasinghe (Ananda); C. Wijmenga (Cisca); B.H.W. Wolffenbuttel (Bruce H.W.); M. Yokota (Mitsuhiro); W. Zheng (Wei); D. Zhu (Dingliang); P. Vineis (Paolo); S.A. Kyrtopoulos (Soterios A.); J.C.S. Kleinjans (Jos C.S.); M.I. McCarthy (Mark); R. Soong (Richie); C. Gieger (Christian); J. Scott (James); Y.Y. Teo (Yik Ying); J. He (Jiang); P. Elliott (Paul); E.S. Tai (Shyong); P. van der Harst (Pim); J.S. Kooner (Jaspal S.); J.C. Chambers (John)

    2015-01-01

    textabstractWe carried out a trans-ancestry genome-wide association and replication study of blood pressure phenotypes among up to 320,251 individuals of East Asian, European and South Asian ancestry. We find genetic variants at 12 new loci to be associated with blood pressure (P = 3.9 × 10 -11 to 5

  7. Investigating University Students' Preferences to Science Communication Skills: A Case of Prospective Science Teacher in Indonesia

    Science.gov (United States)

    Suprapto, Nadi; Ku, Chih-Hsiung

    2016-01-01

    The purpose of this study was to investigate Indonesian university students' preferences to science communication skills. Data collected from 251 students who were majoring in science education program. The Learning Preferences to Science Communication (LPSC) questionnaire was developed with Indonesian language and validated through an exploratory…

  8. Anxiety, depression, and perception of early parenting: A meta-analysis. Erratum

    NARCIS (Netherlands)

    Gerlsma, C.; Emmelkamp, P.M.G.; Arrindell, W.A.

    1991-01-01

    Reports an error in the original article by C. Gerlsma et al (Clinical Psychology Review, 1990, Vol 10[3], 251-277). Corrections are made to the 1st and 3rd formulas on page 257 and to Table 1, page 258, and Table 1, page 261."

  9. University Student Reading Preferences in Relation to the Big Five Personality Dimensions

    Science.gov (United States)

    Schutte, Nicola S.; Malouff, John M.

    2004-01-01

    In this study 251 Australian university students, with a mean age of 26.46, indicated their preference for different types of reading material. A subset of 136 participants additionally reported how many hours per week they typically engage in recreational reading and non-recreational reading, indicated their motivation for recreational reading,…

  10. Japan, Russia and the Northern Territories Dispute: Neighbors in Search of a Good Fence

    Science.gov (United States)

    2002-09-01

    Sun: An Environmental History of the Twentieth Century World (New York: W.W. Norton & Co., 2000), 244-251. 293 Boris Reznik, “Mafia and the Sea...Under the Sun: An Environmental History of the Twentieth Century World. New York: W.W. Norton & Co., 2000. Mid-level officials in the Political

  11. Gclust Server: 91011 [Gclust Server

    Lifescience Database Archive (English)

    Full Text Available 91011 TPS_37727 Cluster Sequences Related Sequences(251) 209 e_gw1.15.98.1 1 1.00e-...35 0.0 11.11 0.0 0.0 0.0 0.0 Show 91011 Cluster ID 91011 Sequence ID TPS_37727 Link to cluster sequences Clu

  12. Gclust Server: 141011 [Gclust Server

    Lifescience Database Archive (English)

    Full Text Available 141011 NCR_NCU01077 Cluster Sequences - 251 predicted protein (translation) (252 aa...); no annotation 1 1.00e-99 0.0 0.0 0.0 0.0 0.0 12.5 Show 141011 Cluster ID 141011 Sequence ID NCR_NCU01077

  13. AcEST: DK958680 [AcEST

    Lifescience Database Archive (English)

    Full Text Available YLGL 588 + RS+ +V R LG+ Sbjct: 251 EGR-MLRSKADVFRLLGI 267 >tr|B5CVC7|B5CVC7_9BACE Putative uncharacterized protein OS=Bacteroides ple...beius DSM 17135 GN=BACPLE_00656 PE=4 SV=1 Length = 1370

  14. The Constitutional Review Chamber of the Republic of Estonia : 3-4-1-5-01 decision of 3 March 2001 : review of the petition of Saare County Court to declare clause 25 (1) of Procedure for Privatisation of Land by Auction invalid

    Index Scriptorium Estoniae

    2001-01-01

    Riigikohtu lahendi 3-4-1-5-01 (Saare Maakohtu taotlus kontrollida Vabariigi Valitsuse 06. 11. 96. a. määrusega nr. 268 kinnitatud "Maa enampakkumisega erastamise korra" p. 25.1 vastavus seadusele ja põhiseadusele) tekst inglise keeles

  15. Data of evolutionary structure change: 1AIFA-2AI0O [Confc[Archive

    Lifescience Database Archive (English)

    Full Text Available s_2> 0 n> 1AIF A...n> 1AIFAn> VSSSI----SSSNL n> 2AI0 n>O 2AI...1AIFA-2AI0O 1AIF 2AI0 A O DIQLTQSPAFMAASPGEKVTITCSVSSSI----SSSNLH...e>SER CA 251 SER CA 276 SER CA 258 ASN CA 337 LEU CA 410

  16. Data of evolutionary structure change: 1AIFA-2AI0L [Confc[Archive

    Lifescience Database Archive (English)

    Full Text Available 2> 0 n> 1AIF n>A ...n>1AIFAn> VSSSI----SSSNL...n> 2AI0 n>L 2AI...1AIFA-2AI0L 1AIF 2AI0 A L DIQLTQSPAFMAASPGEKVTITCSVSSSI----SSSNLH...SER CA 251 SER CA 276 SER CA 258 ASN CA 337 LEU CA 410

  17. The Impact of Imports and Exports on a Country's Quality of Life

    Science.gov (United States)

    Sirgy, M. Joseph; Lee, Dong-Jin; Miller, Chad; Littlefield, James E.; Atay, Eda Gurel

    2007-01-01

    This paper is a sequel to Sirgy et al. ("Social Ind. Res." 68(3) (2004) 251), "The Impact of Globalization on a Country's Quality of Life: Toward an Integrated Model" published in "Social Indicators Research." That paper conceptualized globalization in terms of the free flow of four major components: (1) goods and services, (2) people, (3)…

  18. Repair, Evaluation, Maintenance, and Rehabilitation Research Program. Development of Nondestructive Testing Systems for In Situ Evaluation of Concrete Structures

    Science.gov (United States)

    1987-12-01

    ýpt of diffraction (Sears and Zemansky 1957) must be con- sidered if one is to understand the generation of surface waves. Even when one used the...way Research Program Report 251, Transportation Research Board, Washington, DC. Sears, F. W., and Zemansky , M. W. 1957. UniversLiy Physics, Addison

  19. The involvement of hematopoietic pre-B cell leukemia transcription factor-interacting protein in regulating epithelial-mesenchymal transition of human spinal glioblastoma.

    Science.gov (United States)

    Wang, Deliang; Wang, Li; Zhou, Yi; Zhao, Xinjun; Xiong, Hui

    2016-05-01

    To date, hematopoietic pre-B cell leukemia transcription factor-interacting protein (HPIP), a co-repressor for the transcription factor PBX, has been involved into the initiation and onset in a wide variety of cancers. However, the molecular mechanisms underlying HPIP-induced epithelial-mesenchymal transition (EMT) in the spinal glioblastoma have been under investigation. In the present study, spinal glioblastoma tissues, U87, and U251 cell lines were used and subjected to in vitro assays, such as RT-PCR, and Western blot. Here, in vitro assays revealed that HPIP mRNA and protein were highly expressed in five cases of spinal glioblastoma tissues, compared with non-tumor tissues. Subsequently, in vitro experiments demonstrated HPIP promoted the U87 and U251 cell growth and regulated the G1/S phase transitions in U87 and U251 cell cycle, respectively, accompanied by the increased expression of cyclin A2, cyclin B1, and cyclin D1. Furthermore, HPIP increased the expression of N-cadherin, Slug, and MMP2, and decreased the expression of E-cadherin. By contrast, knockdown of HPIP reversed HPIP-induced EMT biomarkers, migration, and invasion in U87 and U251 cells. In conclusion, our findings identified HPIP plays an important role in the progression and EMT of spinal glioblastoma, by which cell growth is improved. Thus, HPIP gene or protein could act as a useful target in the clinical practice.

  20. Performance Appraisal Interview: A Review of Research.

    Science.gov (United States)

    1987-01-01

    considering subordinates’ effort as well as the results achieved when evaluating performance ( Dornbusch & Scott, 1975; Landy et al. 1978; Morran & Stockton...Journal of Applied Psychology, 66, 248-251. Dornbusch , S.M. & Scott, W.R. (1975). Evaluation and the exercise of authority. San Francisco: Josey-Bass

  1. A viral microRNA down-regulates multiple cell cycle genes through mRNA 5'UTRs.

    Directory of Open Access Journals (Sweden)

    Finn Grey

    Full Text Available Global gene expression data combined with bioinformatic analysis provides strong evidence that mammalian miRNAs mediate repression of gene expression primarily through binding sites within the 3' untranslated region (UTR. Using RNA induced silencing complex immunoprecipitation (RISC-IP techniques we have identified multiple cellular targets for a human cytomegalovirus (HCMV miRNA, miR-US25-1. Strikingly, this miRNA binds target sites primarily within 5'UTRs, mediating significant reduction in gene expression. Intriguingly, many of the genes targeted by miR-US25-1 are associated with cell cycle control, including cyclin E2, BRCC3, EID1, MAPRE2, and CD147, suggesting that miR-US25-1 is targeting genes within a related pathway. Deletion of miR-US25-1 from HCMV results in over expression of cyclin E2 in the context of viral infection. Our studies demonstrate that a viral miRNA mediates translational repression of multiple cellular genes by targeting mRNA 5'UTRs.

  2. Demolition work

    CERN Document Server

    2004-01-01

    Because of demolition work inside Bldg. 251, two containers will be placed on the route Démocrite. For security reasons, the part of this road in front of barrack 553 will be closed to circulation from 5 to 12 November. Thank you, in advance for your understanding. PH-SMI

  3. File list: ALL.Bld.05.AllAg.Leukemic_bone_marrow [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available ALL.Bld.05.AllAg.Leukemic_bone_marrow mm9 All antigens Blood Leukemic bone marrow S...251 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/ALL.Bld.05.AllAg.Leukemic_bone_marrow.bed ...

  4. Dicty_cDB: Contig-U05046-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available one CH251-683O6 from chromo... 34 0.063 11 ( AC212312 ) Solanum lycopersicum cv. Heinz 1706, chromosome 5......Cont... 34 1.3 11 ( AC232763 ) Solanum lycopersicum cv. Heinz 1706, chromosome 5... 30 1.3 10 ( AC232503 ) B

  5. AcEST: BP917683 [AcEST

    Lifescience Database Archive (English)

    Full Text Available ant alignments: (bits) Value sp|O75643|U520_HUMAN U5 small nuclear ribonucleoprotein 200 kDa ... 251 2e-66 sp|Q9VUV... +L +E Sbjct: 506 GAGKTNVALMCMLREIGKHINMDGTINVDDFKIIYIAPMRSLVQE 550 >sp|Q9VUV9|U5

  6. A Taxonomy for Insourcing in the Aerospace Industry

    Science.gov (United States)

    2011-06-01

    jars. This mild warmth caused the jars to expand ever so slightly, causing the lids not to fit. This knowledge was silent and there was no one there...No literature was found on the interpersonal relationships across corporations in business situations. 2.5.1 Transactional Relationships and

  7. A Study on Chocolate Consumption in Prospective Teachers

    Science.gov (United States)

    Ozgen, Leyla

    2016-01-01

    This study was planned and conducted to determine the chocolate consumption habits of prospective teachers. The study population was comprised of students attending the Faculty of Education at Gazi University in Ankara and the sample consisted of 251 prospective teachers selected with simple random sampling. 96.4% and 3.6% of the prospective…

  8. Dicty_cDB: Contig-U14709-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available , comple... 44 3.2 1 ( AF503580 ) Mungbean yellow mosaic India virus-[Cowpea] segme... 44 3.2 1 ( BV338774 ) S230P6496RA5.T0 Rottweil...er Canis familiaris STS g... 44 3.2 1 ( AC159037 ) Pan troglodytes BAC clone CH251-

  9. 77 FR 18305 - Agency Information Collection; Activity Under OMB Review; Report of Passengers Denied Confirmed...

    Science.gov (United States)

    2012-03-27

    ... information is available in the Air Travel Consumer Report at: http://airconsumer.ost.dot.gov/reports/index.htm . The Air Travel Consumer Report is also sent to newspapers, magazines, and trade journals. The...; Report of Passengers Denied Confirmed Space--BTS Form 251 AGENCY: Research & Innovative...

  10. The Roach muscle bundle and umbilical cord coiling

    NARCIS (Netherlands)

    de Laat, Monique W. M.; Nikkels, Peter G. J.; Franx, Arie; Visser, Gerard H. A.

    2007-01-01

    Objective: To determine if presence of the Roach muscle, a small muscle bundle tying just beside the umbilical artery, contributes to umbilical cord coiling. Methods: 251 umbilical cords were examined. The umbilical coiling index (UCI) was calculated as the number of coils divided by the cord length

  11. AcEST: DK962740 [AcEST

    Lifescience Database Archive (English)

    Full Text Available ocara canis G... 249 9e-66 sp|P50880|RL3_CAEEL 60S ribosomal protein L3 OS=Caenorha...-67 sp|P59671|RL3_NEUCR 60S ribosomal protein L3 OS=Neurospora crass... 251 2e-66 sp|P49149|RL3_TOXCA 60S ribosomal protein L3 OS=Tox

  12. Dicty_cDB: Contig-U05654-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available 59 ) 1108770679965 New World Screwworm Egg 9261 ESTs C... 38 0.29 2 ( FG301193 ) 1108799272607 New World Scr...31-01-01-1... 38 0.21 2 ( AC159651 ) Pan troglodytes BAC clone CH251-495E21 from chrom... 40 0.28 5 ( FG2846

  13. 41 CFR 101-25.110-2 - Tires obtained through Federal Supply Schedules or regional term contracts.

    Science.gov (United States)

    2010-07-01

    ... 41 Public Contracts and Property Management 2 2010-07-01 2010-07-01 true Tires obtained through... REGULATIONS SUPPLY AND PROCUREMENT 25-GENERAL 25.1-General Policies § 101-25.110-2 Tires obtained through Federal Supply Schedules or regional term contracts. When tire manufacturers ship tires direct...

  14. Twelve potential fibrosis markers to differentiate mild liver fibrosis from cirrhosis in patients infected with chronic hepatitis C genotype 1

    DEFF Research Database (Denmark)

    Andersen, Ellen Sloth; Ruhwald, M; Moessner, B

    2011-01-01

    activator (suPAR), monokine induced by ¿-interferon (MIG), human hepatocyte growth factor (HGF), insulin, interleukin 6 (IL-6), interleukin 1-ß (IL-1ß), leptin, and nerve growth factor (NGF) were analyzed. Concentrations of TNF-a (median 15.0 vs. 25.1 pg/ml, area under the receiver operating characteristic...

  15. Characterization of epidemic IncI1-Iγ plasmids harboring ambler class A and C genes in Escherichia coli and Salmonella enterica from animals and humans

    NARCIS (Netherlands)

    Smith, Hilde; Bossers, Alex; Harders, Frank; Wu, Guanghui; Woodford, Neil; Schwarz, Stefan; Guerra, Beatriz; Rodríguez, Irene; Essen-Zandbergen, Van Alieda; Brouwer, Michael; Mevius, Dik

    2015-01-01

    The aim of the study was to identify the plasmid-encoded factors contributing to the emergence and spread of epidemic IncI1-Iγ plasmids obtained from Escherichia coli and Salmonella enterica isolates from animal and human reservoirs. For this, 251 IncI1-Iγ plasmids carrying various extended-spect

  16. Arsenic resistance in Campylobacter spp. isolated from retail poultry products.

    Science.gov (United States)

    Sapkota, Amy R; Price, Lance B; Silbergeld, Ellen K; Schwab, Kellogg J

    2006-04-01

    Organoarsenicals are commonly used for growth promotion in U.S. poultry production. Susceptibilities to arsenite, arsenate, and the organoarsenical roxarsone were measured in 251 Campylobacter isolates from conventional and antimicrobial-free retail poultry products. Isolates from conventional poultry products had significantly higher roxarsone MICs (z = 8.22; P < 0.0001).

  17. Rose Atoll Site 27P 2/21/2012 41-42M

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — One-meter-square (1 meter x 1 meter) benthic substrate at Rose Atoll, site 27P (14 33.038S, 168 09.251W), between 41 and 42 meters along a permanent transect.

  18. A Factor Analysis Study of Intellectual Development in Deaf and Hearing Children.

    Science.gov (United States)

    Zweibel, Abraham; Mertens, Donna M.

    The Snijders-Oomen Nonverbal Intelligence Test (SON) was administered to 251 deaf children (6-15 years old) and 101 hearing children (10-12 years old) in Israel. The SON was judged appropriate for measuring cognitive functioning in the deaf because it requires no verbal instructions or responses and includes a measure of abstract thinking ability.…

  19. The Thirteenth and Fourteenth Amendments: Constitutional Authority for Federal Legislation Against Private Sex Discrimination

    Science.gov (United States)

    Calhoun, Emily

    1977-01-01

    Court cases are cited to indicate that twentieth century federal legislation does not prohibit private discrimination based on sex as rigorously as it does that based on race. Enforcement measures afforded by the thirteenth and fourteenth amendments are noted. For journal availability see HE 509 251. (LBH)

  20. The Life-of-Type Inventory Decision for Diminishing Manufacturing Sources Items: A Sensitivity Study.

    Science.gov (United States)

    1982-09-01

    34safety level" of stock for these items. The VSL formula used by DESC is based upon "Model IV" of Presutti and Trepp (the reader is referred to 33:248...Richard C. Trepp . "More Ado About Economic Order Quantities (EOQ)," Naval Research Logistics Quarterly, Vol. 17, No. 2 (June 1970), pp. 243-251. 34

  1. The inhibitory effect of combination treatment with leptin and cannabinoid CB1 receptor agonist on food intake and body weight gain is mediated by serotonin 1B and 2C receptors.

    Science.gov (United States)

    Wierucka-Rybak, M; Wolak, M; Juszczak, M; Drobnik, J; Bojanowska, E

    2016-06-01

    Previous studies reported that the co-injection of leptin and cannabinoid CB1 receptor antagonists reduces food intake and body weight in rats, and this effect is more profound than that induced by these compounds individually. Additionally, serotonin mediates the effects of numerous anorectic drugs. To investigate whether serotonin interacts with leptin and endocannabinoids to affect food intake and body weight, we administered 5-hydroxytryptamine(HT)1B and 5-hydroxytryptamine(HT)2C serotonin receptor antagonists (3 mg/kg GR 127935 and 0.5 mg/kg SB 242084, respectively) to male Wistar rats treated simultaneously with leptin (100 μg/kg) and the CB1 receptor inverse agonist AM 251 (1 mg/kg) for 3 days. In accordance with previous findings, the co-injection of leptin and AM 251, but not the individual injection of each drug, resulted in a significant decrease in food intake and body weight gain. Blockade of the 5-HT1B and 5-HT2C receptors completely abolished the leptin- and AM 251-induced anorectic and body-weight-reducing effects. These results suggest that serotonin mediates the leptin- and AM 251-dependent regulation of feeding behavior in rats via the 5-HT1B and 5-HT2C receptors.

  2. 29 CFR 1910.901-1910.999 - [Reserved

    Science.gov (United States)

    2010-07-01

    ... clothing and equipment .1045(j) Recordkeeping .1045(q) Regulated areas .1045(f) Respiratory protection...) Brazing: (see also Welding) .252 Definitions .251 Standards Sources .256 Breast Derricks: (see also... compliance .1044(g) Notification of use .1044(d) Observation of monitoring .1044(q) Permissible...

  3. Ion Channels in Leukocytes

    Science.gov (United States)

    1991-07-01

    muscle k142), heart muscle (80), bo- are released. In recent years much has been learned vine pulmonar arter endothelial cells (251), and rat about the...channel analysbib of Lt. Potassium permeability in HeLa cancer BioL Chem. 265: 142416-141263, 1990. cells. evidence for a calcium-a’tivated potassium

  4. Trans-ancestry genome-wide association study identifies 12 genetic loci influencing blood pressure and implicates a role for DNA methylation

    DEFF Research Database (Denmark)

    Kato, Norihiro; Loh, Marie; Takeuchi, Fumihiko

    2015-01-01

    We carried out a trans-ancestry genome-wide association and replication study of blood pressure phenotypes among up to 320,251 individuals of East Asian, European and South Asian ancestry. We find genetic variants at 12 new loci to be associated with blood pressure (P = 3.9 × 10(-11) to 5.0 × 10(...

  5. 76 FR 44400 - Submission for OMB Review; Comment Request

    Science.gov (United States)

    2011-07-25

    ..., IRS is hoping to leverage the theory and principles of social marketing. Social marketing principles and practices apply marketing principles to social programs. This data will provide the IRS with... currently approved collection. Title: CS-10-251--Prepaid Card Marketing Customer Survey. Abstract:...

  6. The U.S. Air Service in World War I. Volume IV. Postwar Review,

    Science.gov (United States)

    1979-01-01

    the Hispano- Suiza motor by experience and by continued experiments, the efficiency of the squadron has been impaired and many offers for volunteer...Clerget; 32 faults in; 245 Hispano- Suiza ; 76 Le Rhone 32 Libierty. 20. 87. 89. 131. 155. 177-178, 251. 258 Index 581 Monosoupape 28, 32 protection., for

  7. Dieting, Dietary Restraint, and Binge Eating Disorder among Overweight Adolescents in Turkey

    Science.gov (United States)

    Bas, Murat; Bozan, Nuray; Cigerim, Nevin

    2008-01-01

    The purpose of this study was to determine the relationship among dieting, dietary restraint, disinhibition, hunger, and binge eating among overweight adolescent girls. Participants were 743 overweight adolescent girls between 16 and 19 years of age. The mean BMI was 24.9 [+ or -] 0.8 kg/[m[superscript 2] in the low-restraint group and 25.1 [+ or…

  8. Topical Meeting on Laser and Optical Remote Sensing: Instrumentation and Techniques Technical Digest Held in North Falmoth, Massachusetts on September 28-October 1, 1987. Volume 18.

    Science.gov (United States)

    1988-08-01

    of four wavelengths was shown. References 1) L.Pantani, I.Pippi: Optica Acta, 30, 1473 (1983) 2) L.Pantani, I.Pippi: "The differential reflectance...the time domain. JOSA A, 1, p251-262. 5. Malacara , D., 1978: Optical Shop Testing, Wiley and Sons, NY, NY. 6. Winker 9. M., 1986: Digest of the

  9. Airport Activity Statistics of Certificated Route Air Carriers: Calendar Year 1989

    Science.gov (United States)

    1989-01-01

    Continued COMMUNITY TO AL ................... Domestic ....... S 60085 11536 20.12 20.12 135.48 NS 156 AS 60241 11536 20.12 20.12 135.48 EAGLE N 000...TYPES.,,.- 2522 4 2526 2615 2519 96.32 CGOAMUNIIY TOTAL_............... Domestic. CESSNA 404.. 251 258 DIN _ _ 2468 2468 B-737-300 54 4 58 ALL TYPES

  10. SIRT1 genetic variation is related to BMI and risk of obesity

    NARCIS (Netherlands)

    M.C. Zillikens (Carola); J.B.J. van Meurs (Joyce); F. Rivadeneira Ramirez (Fernando); N. Amin (Najaf); A. Hofman (Albert); B.A. Oostra (Ben); E.J.G. Sijbrands (Eric); J.C.M. Witteman (Jacqueline); H.A.P. Pols (Huib); P. Tikka-Kleemola (Päivi); A.G. Uitterlinden (André)

    2009-01-01

    textabstractOBJECTIVE - SIRT1 has pleiotropic metabolic functions. We investigated whether SIRT1 genetic variation is associated with obesity. RESEARCH DESIGN AND METHODS - In 6,251 elderly subjects from the prospective, population-based Rotterdam Study, three single nucleotide polymorphisms (SNPs)

  11. Smallpox Antiviral Drug

    Science.gov (United States)

    2007-01-01

    strategies is essential. ACKNOWLEDGMENTS We thank Robert Jordan, Sean Amberg, and Tove’ Bolken for critical reviews of the manuscript. REFERENCES...part II. Adverse events. Clin Infect Dis 37:251–271. Ghosn J, Chaix ML, Peytavin G, Rey E, Bresson JL, Goujard C, Katlama C, Viard JP, Treluyer JM

  12. AcEST: BP918395 [AcEST

    Lifescience Database Archive (English)

    Full Text Available 31 2.2 sp|O43474|KLF4_HUMAN Krueppel-like factor 4 OS=Homo sapiens GN=K... 31 2.2 sp|Q13477|MADCA_HUMAN Mucosal address... C H G Sbjct: 251 SKGSPDGSHPVVVAPYNGGPPRTCPKIKQEAVSSCTHLG 289 >sp|Q13477|MADCA_HUMAN Mucosal address

  13. 78 FR 59659 - Correction to the Final Environmental Impact Statement/Overseas Environmental Impact Statement...

    Science.gov (United States)

    2013-09-27

    ... of the corrected HSTT Final EIS/OEIS are available for public review at the following libraries: 1. Lihue Public Library, 4344 Hardy Street, Lihue, Hawaii 96766. 2. Wailuku Public Library, 251 High Street, Wailuku, Hawaii 96793. 3. Hilo Public Library, 300 Waianuenue Avenue, Hilo, Hawaii 96720. 4....

  14. Science Park of Universities in Jiangsu Province Nanjing Jingang Science Pioneering Center

    Institute of Scientific and Technical Information of China (English)

    2004-01-01

    <正> The Nanjing Jingang Science Pioneering Center was estab-lished in August 2000. It is located at Tower A, Jingang Building, 251 Heyan Road, Nanjing, and has a 28-story and 25,000m2 of space for the incubation of projects of

  15. 78 FR 80415 - To Take Certain Actions Under the African Growth and Opportunity Act and for Other Purposes

    Science.gov (United States)

    2013-12-31

    ... Opportunity Act and for Other Purposes Presidential Documents Federal Register / Vol. 78 , No. 251 / Tuesday... December 23, 2013 To Take Certain Actions Under the African Growth and Opportunity Act and for Other... meeting the requirements described in section 506A(a)(1) of the Trade Act of 1974, as amended (the...

  16. Rose Atoll Site 27P 2/21/2012 21-22M

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — One-meter-square (1 meter x 1 meter) benthic substrate at Rose Atoll, site 27P (14 33.038S, 168 09.251W), between 21 and 22 meters along a permanent transect.

  17. Rose Atoll Site 27P 8/24/1999 31-32M

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — One-meter-square (1 meter x 1 meter) benthic substrate at Rose Atoll, site 27P (14 33.038S, 168 09.251W), between 31 and 32 meters along a permanent transect.

  18. Rose Atoll Site 27P 6/21/2005 (42)M

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — One-meter-square (1 meter x 1 meter) benthic substrate at Rose Atoll, site 27P (14 33.038S, 168 09.251W), at meter 42 along a permanent transect.

  19. Rose Atoll Site 27P 2/21/2012 30-31M

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — One-meter-square (1 meter x 1 meter) benthic substrate at Rose Atoll, site 27P (14 33.038S, 168 09.251W), between 30 and 31 meters along a permanent transect.

  20. Pattern of marine mass extinction near the Permian-Triassic boundary in South China.

    Science.gov (United States)

    Jin, Y G; Wang, Y; Wang, W; Shang, Q H; Cao, C Q; Erwin, D H

    2000-07-21

    The Meishan section across the Permian-Triassic boundary in South China is the most thoroughly investigated in the world. A statistical analysis of the occurrences of 162 genera and 333 species confirms a sudden extinction event at 251.4 million years ago, coincident with a dramatic depletion of delta13C(carbonate) and an increase in microspherules.

  1. Observations of High-Speed Plasma Flow Near the Earth’s Magnetopause: Evidence for Reconnection.

    Science.gov (United States)

    1981-06-01

    shown in Plate la which covers the first six hours of ISEFE -I measturements on 8 September 1978 (day 251). The E-t spectroqrams disolay detector responses...Figure 5. Perspective plot of the three-dimensional ion velocity distribution in the boundary layer sampled by the ISEF -l r,,PEFDEA on R September 1978

  2. AcEST: DK946098 [AcEST

    Lifescience Database Archive (English)

    Full Text Available ng domain-containing ... 30 5.3 sp|Q5U3U6|BORA_DANRE Protein aurora borealis OS=Danio rerio GN=b... 30 6.9 >... H ERE+ P+TA G G Sbjct: 251 QDLERAG-LRQRE---RRGRRLQEHAERESQPATATGAAQG 287 >sp|Q5U3U6|BORA_DANRE Protein aurora borealis

  3. The Cannabinoid System in the Retrosplenial Cortex Modulates Fear Memory Consolidation, Reconsolidation, and Extinction

    Science.gov (United States)

    Sachser, Ricardo Marcelo; Crestani, Ana Paula; Quillfeldt, Jorge Alberto; e Souza, Tadeu Mello; de Oliveira Alvares, Lucas

    2015-01-01

    Despite the fact that the cannabinoid receptor type 1 (CB1R) plays a pivotal role in emotional memory processing in different regions of the brain, its function in the retrosplenial cortex (RSC) remains unknown. Here, using contextual fear conditioning in rats, we showed that a post-training intra-RSC infusion of the CB1R antagonist AM251

  4. 78 FR 15007 - Notice of Open House-Draft Environmental Impact Statement for Revised Water Control Manuals for...

    Science.gov (United States)

    2013-03-08

    ... Revised Water Control Manuals for the Alabama-Coosa-Tallapoosa River Basin AGENCY: Department of the Army... Alabama-Coosa-Tallapoosa River Basin (ACT) Water Control Master Manual (Master Manual). The public comment... CONTACT: Mr. Lewis Sumner at telephone (251) 694-3857. SUPPLEMENTARY INFORMATION: The Master...

  5. 8 CFR 1280.53 - Civil monetary penalties inflation adjustment.

    Science.gov (United States)

    2010-01-01

    ... adjustment. 1280.53 Section 1280.53 Aliens and Nationality EXECUTIVE OFFICE FOR IMMIGRATION REVIEW, DEPARTMENT OF JUSTICE IMMIGRATION REGULATIONS IMPOSITION AND COLLECTION OF FINES § 1280.53 Civil monetary...; Reports of Illegal Landings: from $200 to $220 for each alien not reported in accordance with § 251;...

  6. 8 CFR 280.53 - Civil monetary penalties inflation adjustment.

    Science.gov (United States)

    2010-01-01

    ... adjustment. 280.53 Section 280.53 Aliens and Nationality DEPARTMENT OF HOMELAND SECURITY IMMIGRATION...; Reports of Illegal Landings: from $200 to $220 for each alien not reported in accordance with § 251; and... Alien Crewmen Into United States with Intent to Evade Immigration Laws: from a $10,000 maximum to a...

  7. Dicty_cDB: SFB147 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available 5 DQ673411_1( DQ673411 |pid:none) Diaphorina citri putative ribosoma... 48 9e-05 EU934275_1( EU934275 |pid:none) TSA: Anopheles darli...ngi AD-251 40S... 48 9e-05 EF634885_1( EF634885 |pid:none) Pristionchus sp. 10 RS51

  8. Comprehensive analysis of varicella-zoster virus proteins using a new monoclonal antibody collection

    NARCIS (Netherlands)

    T.L. Roviš (Tihana Lenac); S.M. Bailer (Susanne); V.R. Pothineni (Venkata R); W.J.D. Ouwendijk (Werner ); H. Šimić (Hrvoje); M. Babić (Marina); K. Miklić (Karmela); S. Malić (Suzana); M.C. Verweij; M. Baiker (Martin); O. Gonzalez (Orland); A. Brunn (Albrecht von); R. Zimmer; K. Früh (Klaus); G.M.G.M. Verjans (George); S. Jonjic (Stipan); J. Haasb (Jürgeni)

    2013-01-01

    textabstractVaricella-zoster virus (VZV) is the etiological agent of chickenpox and shingles. Due to the virus's restricted host and cell typetropism and the lack of tools for VZV proteomics, it is one of the least-characterized human herpesviruses. We generated 251monoclonal antibodies (MAbs) again

  9. Waterborne Commerce of the United States, Calendar Year 1980. Part 1. Waterways and Harbors, Atlantic Coast.

    Science.gov (United States)

    1982-09-01

    Canada, the esport statistics reflect fully compiled data for shipments named $1,000 and over combined with estimated data for shipments valted $251-$999...Rivers, Federal and Private Canals, Connecting Channels, and Intracoastal Waterways The terms "imports." " esports ." "coastwise receipts," "coastwise

  10. Congenital heart disease

    Institute of Scientific and Technical Information of China (English)

    1997-01-01

    970296 Evaluating the degree of pulmonary vascularlesions in congenital heart disease with selective pul-monary angiography. PAN Shiwei(潘世伟), et al.Fuwai Hosp, CAMS & PUMC, Beijing, 100037. Chin JCardiol 1997; 25(1): 39-41. Objective: To evaluate the degree of pulmonary vas-

  11. A Comprehensive Survey on the Status of Social and Professional Issues in United States Undergraduate Computer Science Programs and Recommendations

    Science.gov (United States)

    Spradling, Carol; Soh, Leen-Kiat; Ansorge, Charles J.

    2009-01-01

    A national web-based survey was administered to 700 undergraduate computer science (CS) programs in the United States as part of a stratified random sample of 797 undergraduate CS programs. The 251 program responses (36% response rate) regarding social and professional issues are presented. This article describes the demographics of the…

  12. 78 FR 42755 - Gulf of Mexico Fishery Management Council; Public Hearings

    Science.gov (United States)

    2013-07-17

    ... and Baton Rouge, LA; D' Iberville, MS; Mobile, AL; Corpus Christi and Texas City, TX. Council address... regional management, red snapper would remain a federally managed species subject to federal conservation... South Water Street, Mobile, AL 36602, telephone: (251) 438-4000. Monday, August 12, 2013, Hilton...

  13. Murray Pittock, ed., The Edinburgh Companion to Scottish Romanticism.

    Directory of Open Access Journals (Sweden)

    Manfred Malzahn

    2013-10-01

    Full Text Available Murray Pittock, ed., The Edinburgh Companion to Scottish Romanticism. Edinburgh: Edinburgh University Press, 2011. Pp. 251. ISBN 978-0-7486-3845-1 (hardback. £ 65.00. ISBN 978-0-7486-3846-8 (paperback. £ 21.99.

  14. 40 CFR 264.253 - Response actions.

    Science.gov (United States)

    2010-07-01

    ....253 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES Waste Piles § 264.253 Response actions. (a) The owner or operator of waste pile units subject to § 264.251...

  15. 40 CFR 264.252 - Action leakage rate.

    Science.gov (United States)

    2010-07-01

    ... 264.252 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES Waste... for waste pile units subject to § 264.251(c) or (d). The action leakage rate is the maximum...

  16. Dicty_cDB: SLB154 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available iocidaris tuberculata SOX1 prot... 35 3.8 CP000496_474( CP000496 |pid:none) Pichia ...25_1( AY344225 |pid:none) Candida glabrata Epa5p (EPA5) and ... 35 3.8 AY532156_1( AY532156 |pid:none) Hel

  17. Individual Differences and Learning Performance in Computer-based Training

    Science.gov (United States)

    2011-02-01

    Navigation in hypermedia learning systems: experts vs. novices. Computers in Human Behavior , 22, 251–266. Chi, M. T. H., Glaser, R., & Rees, E...communication technologies on performance in a Web-based learning program. Computers in Human Behavior , 22(6), 962-970. Shivpuri, S., Schmitt, N., Oswald

  18. Exploring Academic Motivation, Academic Self-Efficacy and Attitudes toward Teaching in Pre-Service Early Childhood Education Teachers

    Science.gov (United States)

    Bedel, Emine Ferda

    2016-01-01

    This study is designed to explore academic motivation, academic self-efficacy and attitudes toward teaching in pre-service early childhood education teachers and to investigate the relationships among those variables. Data were gathered through questionnaires administered to 251 pre-service early childhood education teachers. Results indicated…

  19. 78 FR 38842 - Definition of a Ski Area

    Science.gov (United States)

    2013-06-28

    ... Forest Service 36 CFR Part 251 RIN 0596-AD12 Definition of a Ski Area AGENCY: Forest Service, USDA. ACTION: Interim final rule. SUMMARY: The Forest Service is amending the definition of a ski area in its regulations to make it consistent with the authority in section 3 of the Ski Area Recreational...

  20. Longitudinal Predictors of Chinese Word Reading and Spelling among Elementary Grade Students

    Science.gov (United States)

    Yeung, Pui-Sze; Ho, Connie Suk-Han; Wong, Yau-Kai; Chung, Kevin Kien-Hoa; Lo, Lap-Yan

    2013-01-01

    The longitudinal predictive power of four important reading-related skills (phonological skills, rapid naming, orthographic skills, and morphological awareness) to Chinese word reading and writing to dictation (i.e., spelling) was examined in a 3-year longitudinal study among 251 Chinese elementary students. Rapid naming, orthographic skills, and…

  1. Modulatory effects by CB1 receptors on rat spinal locomotor networks after sustained application of agonists or antagonists.

    Science.gov (United States)

    Veeraraghavan, P; Nistri, A

    2015-09-10

    Sustained administration of cannabinoid agonists acting on neuronal CB1 receptors (CB1Rs) are proposed for treating spasticity and chronic pain. The impact of CB1Rs on mammalian locomotor networks remains, however, incompletely understood. To clarify how CB1Rs may control synaptic activity and locomotor network function, we used the rat spinal cord in vitro which is an advantageous model to investigate locomotor circuit mechanisms produced by the local central pattern generator. Neither the CB1 agonist anandamide (AEA) nor the CB1R antagonist AM-251 evoked early (3-24h largely impaired locomotor network activity induced by DR stimuli or neurochemicals, and depressed disinhibited bursting without changing reflex amplitude or inducing neurotoxicity even if CB1R immunoreactivity was lowered in the central region. Since CB1R activation usually inhibits cyclic adenosine monophosphate (cAMP) synthesis, we investigated how a 24-h application of AEA or AM-251 affected basal or forskolin-stimulated cAMP levels. While AEA decreased them in an AM-251-sensitive manner, AM-251 per se did not change resting or stimulated cAMP. Our data suggest that CB1Rs may control the circuit gateway regulating the inflow of sensory afferent inputs into the locomotor circuits, indicating a potential site of action for restricting peripheral signals disruptive for locomotor activity.

  2. Dicty_cDB: Contig-U13884-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available _1( AB367930 |pid:none) Theileria sergenti CCTeta gene for... 251 8e-65 CR382139_608( CR382139 |pid:none) De...929 |pid:none) Theileria sergenti CCTeta gene for... 221 8e-56 AB367931_1( AB367931 |pid:none) Theileria serge

  3. Oil Price Hike Boosts CNOOC Revenue%Oil Price Hike Boosts CNOOC Revenue

    Institute of Scientific and Technical Information of China (English)

    2011-01-01

    Q1 production up 26.6 percent CNOOC Limited's first-quarter production increased 26.6 percent to 85.2 million barrels of oil equivalent compared with the same period last year. Output at the Jinzhou 25-1 project commenced in the first quarter, CNOOC sa

  4. 24 CFR 92.206 - Eligible project costs.

    Science.gov (United States)

    2010-04-01

    ... following: (1) For new construction, costs to meet the applicable new construction standards of the participating jurisdiction and the Model Energy Code referred to in § 92.251; (2) For rehabilitation, costs: (i... construction and rehabilitation of multifamily rental housing, costs to construct or rehabilitate laundry...

  5. Improving the Selection, Classification, and Utilization of Army Enlisted Personnel: Annual Report, 1987 Fiscal Year

    Science.gov (United States)

    1989-10-01

    ADA Short RG Gunnery Cremobr 16 CO OF 16S MANPADS Crewmember 16 CO OF 24M VULCAN System Mech 23 M14 EL 24N CHAPARRAL System Mech 23 M14 EL 251 ADA...Rig rifle. 00 sachimegun, LAW, eones and booby traps, and grenades. NOVEIIEMT AIIO &flVIVAi. 10 FIELD: Tesis related to battlefield survivel is

  6. Kirjad vallutatud Hiinast / Karl Rumor

    Index Scriptorium Estoniae

    Rumor, Karl, pseud., 1886-1971

    2008-01-01

    1937. a. alanud Hiina-Jaapani sõjast, poliitlistest oludest ja revolutsioonist Hiinas, hiinlastest ja Hiina suhetest teiste riikidega. Varem ilmunud: Uus Eesti 19.-20. aug, 1938, nr. 226-227; 12.-13., 16. sept. 1938, nr. 250-251, 254

  7. EST Table: DC551726 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available ecursor dbj|BAA06160.1| humoral lectin prepropeptide [Bombyx mori] prf||2106140A hemocytin 10/09/02 31 %/251...cursor [Bombyx mori] sp|P98092.1|HMCT_BOMMO RecName: Full=Hemocytin; AltName: Full=Humoral lectin; Flags: Pr

  8. Cumulative lactate and hospital mortality in ICU patients

    NARCIS (Netherlands)

    van Beest, Paul A.; Brander, Lukas; Jansen, Sebastiaan P. A.; Rommes, Johannes H.; Kuiper, Michael A.; Spronk, Peter E.

    2013-01-01

    Background: Both hyperlactatemia and persistence of hyperlactatemia have been associated with bad outcome. We compared lactate and lactate-derived variables in outcome prediction. Methods: Retrospective observational study. Case records from 2,251 consecutive intensive care unit (ICU) patients admit

  9. Animal foods, protein, calcium and prostate cancer risk: the European Prospective Investigation into Cancer and Nutrition.

    NARCIS (Netherlands)

    Allen, N.E.; Key, T.J.; Appleby, P.N.; Travis, R.C.; Roddam, A.W.; Tjonneland, A.; Johnsen, N.F.; Overvad, K.; Linseisen, J.; Rohrmann, S.; Boeing, H.; Pischon, T.; Bueno-De-Mesquita, H.B.; Kiemeney, L.; Tagliabue, G.; Palli, D.; Vineis, P.; Tumino, R.; Trichopoulou, A.; Kassapa, C.; Trichopoulos, D.; Ardanaz, E.; Larranaga, N.; Tormo, M.J.; Gonzalez, C.A.; Quiros, J.R.; Sanchez, M.J.; Bingham, S.; Khaw, K.T.; Manjer, J.; Berglund, G.; Stattin, P.; Hallmans, G.; Slimani, N.; Ferrari, P.; Rinaldi, S.; Riboli, E.

    2008-01-01

    We examined consumption of animal foods, protein and calcium in relation to risk of prostate cancer among 142 251 men in the European Prospective Investigation into Cancer and Nutrition. Associations were examined using Cox regression, stratified by recruitment centre and adjusted for height, weight

  10. Animal foods, protein, calcium and prostate cancer risk : the European Prospective Investigation into Cancer and Nutrition

    NARCIS (Netherlands)

    Allen, N. E.; Key, T. J.; Appleby, P. N.; Travis, R. C.; Roddam, A. W.; Tjonneland, A.; Johnsen, N. F.; Overvad, K.; Linseisen, J.; Rohrmann, S.; Boeing, H.; Pischon, T.; Bueno-de-Mesquita, H. B.; Kiemeney, L.; Tagliabue, G.; Palli, D.; Vineis, P.; Tumino, R.; Trichopoulou, A.; Kassapa, C.; Trichopoulos, D.; Ardanaz, E.; Larranaga, N.; Tormo, M-J; Gonzalez, C. A.; Quiros, J. R.; Sanchez, M-J; Bingham, S.; Khaw, K-T; Manjer, J.; Berglund, G.; Stattin, P.; Hallmans, G.; Slimani, N.; Ferrari, P.; Rinaldi, S.; Riboli, E.

    2008-01-01

    We examined consumption of animal foods, protein and calcium in relation to risk of prostate cancer among 142 251 men in the European Prospective Investigation into Cancer and Nutrition. Associations were examined using Cox regression, stratified by recruitment centre and adjusted for height, weight

  11. Rose Atoll Site 27P 6/21/2005 (25)M

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — One-meter-square (1 meter x 1 meter) benthic substrate at Rose Atoll, site 27P (14 33.038S, 168 09.251W), at meter 25 along a permanent transect.

  12. 76 FR 81998 - Methodology for Low Power/Shutdown Fire PRA

    Science.gov (United States)

    2011-12-29

    ...) 492-3446. FOR FURTHER INFORMATION CONTACT: Felix E. Gonzalez, Division of Risk Analysis, Office of...: (301) 251- 7596, email: Felix.Gonzalez@nrc.gov or Hugh W. Woods, Division of Risk Analysis, Office of... quantitatively analyzing fire risk in commercial nuclear power plants during low power operation and...

  13. QTL Information Table: 637 [Q-TARO

    Lifescience Database Archive (English)

    Full Text Available tns6 Morphological trait Panicle/flower Total number of spikelets per panicle RFLP C)Interval... Z., Huang, N., and Zheng, K.L. (1995). Interval mapping of QTLs for yield and other related characters in rice. Rice Genetics Newsletter 12, 251-253. ...

  14. QTL Information Table: 635 [Q-TARO

    Lifescience Database Archive (English)

    Full Text Available nfb8 Morphological trait Panicle/flower Number of first branches per panicle RFLP C)Interval...Z., Huang, N., and Zheng, K.L. (1995). Interval mapping of QTLs for yield and other related characters in rice. Rice Genetics Newsletter 12, 251-253. ...

  15. QTL Information Table: 632 [Q-TARO

    Lifescience Database Archive (English)

    Full Text Available ng1 Morphological trait Panicle/flower Number of grains per panicle RFLP C)Interval...g, N., and Zheng, K.L. (1995). Interval mapping of QTLs for yield and other related characters in rice. Rice Genetics Newsletter 12, 251-253. ...

  16. QTL Information Table: 631 [Q-TARO

    Lifescience Database Archive (English)

    Full Text Available np4 Morphological trait Panicle/flower Number of panicles per plant RFLP C)Interval..., N., and Zheng, K.L. (1995). Interval mapping of QTLs for yield and other related characters in rice. Rice Genetics Newsletter 12, 251-253. ...

  17. 76 FR 78808 - Airworthiness Directives; Teledyne Continental Motors (TCM) and Rolls-Royce Motors Ltd. (R-RM...

    Science.gov (United States)

    2011-12-20

    ... Continental Motors (TCM) and Rolls-Royce Motors Ltd. (R-RM) Series Reciprocating Engines AGENCY: Federal... Motors, Inc., PO Box 90, Mobile, AL 36601; phone: 251-438-3411, or go to http://tcmlink.com...: 2011-26-07 Teledyne Continental Motors (TCM) and Rolls-Royce Motors Ltd. (R-RM) Series...

  18. 41 CFR 101-25.110-3 - Tires accompanying new motor vehicles.

    Science.gov (United States)

    2010-07-01

    ... motor vehicles. 101-25.110-3 Section 101-25.110-3 Public Contracts and Property Management Federal...-GENERAL 25.1-General Policies § 101-25.110-3 Tires accompanying new motor vehicles. The tire identifications and recordkeeping regulations issued by the Department of Transportation require each...

  19. Sequence Classification: 783456 [

    Lifescience Database Archive (English)

    Full Text Available Non-TMB Non-TMH Non-TMB Non-TMB Non-TMB Non-TMB >gi|17560486|ref|NP_505710.1| abnormal CATecholamine distrib...ution CAT-4, GTP cyclohydrolase I (25.1 kD) (cat-4) || http://www.ncbi.nlm.nih.gov/protein/17560486 ...

  20. Rose Atoll Site 27P 2/21/2012 9-10M

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — One-meter-square (1 meter x 1 meter) benthic substrate at Rose Atoll, site 27P (14 33.038S, 168 09.251W), between 9 and 10 meters along a permanent transect.

  1. Gene by Environment Investigation of Incident Lung Cancer Risk in African-Americans

    Directory of Open Access Journals (Sweden)

    Sean P. David

    2016-02-01

    Interpretation: These results suggest that chromosome 15q25.1 variants are robustly associated with CPD and lung cancer in African-Americans and that the allelic dose effect of these polymorphisms on lung cancer risk is most pronounced in lighter smokers.

  2. Universal Lagrangian bundles

    NARCIS (Netherlands)

    Sepe, D.

    2013-01-01

    The obstruction to construct a Lagrangian bundle over a fixed integral affine manifold was constructed by Dazord and Delzant (J Differ Geom 26:223–251, 1987) and shown to be given by ‘twisted’ cup products in Sepe (Differ GeomAppl 29(6): 787–800, 2011). This paper uses the topology of universal Lagr

  3. Attachment Styles, Abuse Experiences and Depression

    Science.gov (United States)

    Gunduz, Bulent; Capri, Burhan; Akbay, Sinem Evin; Tunc, Aygul

    2013-01-01

    This study aims to examine the relationship between abuse experiences, depression and attachment styles. The data for this study was collected from 251 students (123 females and 128 males) who attend Mersin University. As a part of the data collection, students completed the Experiences in Affiliation Inventory, Childhood Trauma Scale and Brief…

  4. The NMR restraints grid at BMRB for 5,266 protein and nucleic acid PDB entries.

    NARCIS (Netherlands)

    Doreleijers, J.F.; Vranken, W.F.; Schulte, C.; Lin, J.; Wedell, J.R.; Penkett, C.J.; Vuister, G.W.; Vriend, G.; Markley, J.L.; Ulrich, E.L.

    2009-01-01

    Several pilot experiments have indicated that improvements in older NMR structures can be expected by applying modern software and new protocols (Nabuurs et al. in Proteins 55:483-186, 2004; Nederveen et al. in Proteins 59:662-672, 2005; Saccenti and Rosato in J Biomol NMR 40:251-261, 2008). A recen

  5. ANTIBODIES TO THE PUTATIVE SIV INFECTION-ENHANCING DOMAIN DIMINISH BENEFICIAL-EFFECTS OF AN SIV GP160 VACCINE IN RHESUS MACAQUES

    NARCIS (Netherlands)

    MITCHELL, WM; TORRES, J; JOHNSON, PR; HIRSCH, [No Value; YILMA, T; GARDNER, MB; ROBINSON, WE

    1995-01-01

    Objective: To demonstrate that antibodies against amino acids (aa) 603-622 of the SIV gp41 transmembrane glycoprotein enhance infection of SIV in vivo. Design: A synthetic peptide derived from aa 603-622 of SIVmac251 gp41 was synthesized and tested for immunogenicity in rabbits and SIV-infected rhes

  6. Do Prospective Classroom Teachers Perceive Themselves as Effective and Willing to Teach Young Learners English?

    Science.gov (United States)

    Sad, Süleyman Nihat

    2015-01-01

    This study aimed to investigate the perceived efficacy and willingness levels of prospective classroom teachers to teach English at the primary level. The study was designed as a baseline descriptive survey, followed by complementary correlational and ex post facto models. Participants were 251 prospective classroom teachers. Data was collected…

  7. Drug: D06126 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available 252.164 D06126.gif Antihypertensive CAS: 51274-83-0 PubChem: 47207784 LigandBox: D06126 NIKKAJI: J268.948K A...D06126 Drug Tiamenidine hydrochloride (USAN); Symcor (TN) C8H10ClN3S. HCl 251.0051

  8. Gene-Environment Interaction and Breast Cancer on Long Island, NY

    Science.gov (United States)

    2008-05-01

    SUBJECT TERMS epidemiology, environmental exposures, endocrine disruptors, estrogen receptor genes, gene- environment interaction, methodologic approaches... Environmental Exposures and BPA: Questionnaire data and biomarkers. Breast Cancer and the Environment Research Centers’ Integration Meeting...cosmetic components in reporter cell lines: parabens , UV screens, and musks. J. Toxicol. Environ . Health A 68, 239–251. Gray, L.E., Furr, J., Wilson

  9. Attenuation of morphine antinociceptive tolerance by cannabinoid CB1 and CB2 receptor antagonists.

    Science.gov (United States)

    Altun, Ahmet; Yildirim, Kemal; Ozdemir, Ercan; Bagcivan, Ihsan; Gursoy, Sinan; Durmus, Nedim

    2015-09-01

    Cannabinoid CB1 and CB2 receptor antagonists may be useful for their potential to increase or prolong opioid analgesia while attenuating the development of opioid tolerance. The aim of this study was to investigate the effects of AM251 (a selective CB1 antagonist) and JTE907 (a selective CB2 antagonist) on morphine analgesia and tolerance in rats. Adult male Wistar albino rats weighing 205-225 g were used in these experiments. To constitute morphine tolerance, we used a 3 day cumulative dosing regimen. After the last dose of morphine was injected on day 4, morphine tolerance was evaluated by analgesia tests. The analgesic effects of morphine (5 mg/kg), ACEA (a CB1 receptor agonist, 5 mg/kg), JWH-015 (a CB2 receptor agonist, 5 mg/kg), AM251 (1 mg/kg) and JTE907 (5 mg/kg) were considered at 30-min intervals (0, 30, 60, 90, and 120 min) by tail-flick and hot-plate analgesia tests. Our findings indicate that ACEA and JWH907 significantly increased morphine analgesia and morphine antinociceptive tolerance in the analgesia tests. In contrast, the data suggested that AM251 and JTE907 significantly attenuated the expression of morphine tolerance. In conclusion, we observed that co-injection of AM251 and JTE907 with morphine attenuated expression of tolerance to morphine analgesic effects and decreased the morphine analgesia.

  10. Predicting Intrinsic Motivation

    Science.gov (United States)

    Martens, Rob; Kirschner, Paul A.

    2004-01-01

    Intrinsic motivation can be predicted from participants' perceptions of the social environment and the task environment (Ryan & Deci, 2000)in terms of control, relatedness and competence. To determine the degree of independence of these factors 251 students in higher vocational education (physiotherapy and hotel management) indicated the extent to…

  11. EST Table: FY737608 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available FY737608 E_FL_famL_03C10_F_0 11/11/04 99 %/251 aa ref|NP_001036933.1| molting fluid... carboxypeptidase A [Bombyx mori] dbj|BAD60916.1| molting fluid carboxypeptidase A [Bombyx mori] 11/11/04 45

  12. Domain Modeling: NP_001092103.1 [SAHG[Archive

    Lifescience Database Archive (English)

    Full Text Available NP_001092103.1 chr19 FUSION OF N-TERMINAL DOMAIN OF THE MINOR COAT PROTEIN FROM GEN...E III IN PHAGE M13, AND C-TERMINAL DOMAIN OF E. COLI PROTEIN-TOLA c1tola_ chr19/NP_001092103.1/NP_001092103.1_apo_24-251.pdb swppa 0 ...

  13. Fire Patterns among Ecological Zones in the California Desert, 1984–2013

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — The California desert occupies the southeastern 27% of California (11,028,300 ha, 110,283 km2 or 27,251,610 ac). It includes two ecoregional provinces comprised of...

  14. Domain Expert-Directed Program Optimizations for Accelerated Performance on Heterogeneous Multi-core Processors

    Science.gov (United States)

    2013-12-01

    modulo ) operation as used in Ed25519 ( A←A+BC2255−19 ) in the radix- 251 redundant representation, and will complete the verification of the same...Taiwan University this year. He is a young up-and-coming expert on formal verification and languages, and his impressive resumè include joint work

  15. MARKET WATCH

    Institute of Scientific and Technical Information of China (English)

    HU YUE

    2010-01-01

    @@ Trade Surplus Drops China's trade surplus is shrinking,as exports slowed down in September. Exports rose 25.1 percent in September from a year earlier to $144.99billion,9.3 percentage points slower than August,said the General Administration of Customs.

  16. 14 CFR Appendix I to Part 25 - Installation of an Automatic Takeoff Thrust Control System (ATTCS)

    Science.gov (United States)

    2010-01-01

    ... Appendix I to Part 25—Installation of an Automatic Takeoff Thrust Control System (ATTCS) I25.1General. (a... crew to increase thrust or power. I25.2Definitions. (a) Automatic Takeoff Thrust Control System (ATTCS... Control System (ATTCS) I Appendix I to Part 25 Aeronautics and Space FEDERAL AVIATION...

  17. Some Nonlinear Dynamic Inequalities on Time Scales

    Indian Academy of Sciences (India)

    Wei Nian Li; Weihong Sheng

    2007-11-01

    The aim of this paper is to investigate some nonlinear dynamic inequalities on time scales, which provide explicit bounds on unknown functions. The inequalities given here unify and extend some inequalities in (B G Pachpatte, On some new inequalities related to a certain inequality arising in the theory of differential equation, J. Math. Anal. Appl. 251 (2000) 736--751).

  18. The Separate and Cumulative Effects of TBI and PTSD on Cognitive Function and Emotional Control

    Science.gov (United States)

    2012-10-01

    Diane Swick, Ph.D. Frontal Lobe Injury and Executive Control of Cognition and Emotion. VA Merit renewal funded by CSR &D (Award Number 1I01CX000566...potentials and recognition memory. Trends Cogn Sci, 11(6), 251-257. Shalev, A. Y., Freedman, S., Peri, T., Brandes , D., Sahar, T., Orr, S. P., et al. (1998

  19. Comparison of 6q25 Breast Cancer Hits from Asian and European Genome Wide Association Studies in the Breast Cancer Association Consortium (BCAC)

    NARCIS (Netherlands)

    Hein, Rebecca; Maranian, Melanie; Hopper, John L.; Kapuscinski, Miroslaw K.; Southey, Melissa C.; Park, Daniel J.; Schmidt, Marjanka K.; Broeks, Annegien; Hogervorst, Frans B. L.; Bueno-de-Mesquit, H. Bas; Muir, Kenneth R.; Lophatananon, Artitaya; Rattanamongkongul, Suthee; Puttawibul, Puttisak; Fasching, Peter A.; Hein, Alexander; Ekici, Arif B.; Beckmann, Matthias W.; Fletcher, Olivia; Johnson, Nichola; Silva, Isabel dos Santos; Peto, Julian; Sawyer, Elinor; Tomlinson, Ian; Kerin, Michael; Miller, Nicola; Marmee, Frederick; Schneeweiss, Andreas; Sohn, Christof; Burwinkel, Barbara; Guenel, Pascal; Cordina-Duverger, Emilie; Menegaux, Florence; Truong, Therese; Bojesen, Stig E.; Nordestgaard, Borge G.; Flyger, Henrik; Milne, Roger L.; Arias Perez, Jose Ignacio; Pilar Zamora, M.; Benitez, Javier; Anton-Culver, Hoda; Ziogas, Argyrios; Bernstein, Leslie; Clarke, Christina A.; Brenner, Hermann; Mueller, Heiko; Arndt, Volker; Stegmaier, Christa; Rahman, Nazneen; Seal, Sheila; Turnbull, Clare; Renwick, Anthony; Meindl, Alfons; Schott, Sarah; Bartram, Claus R.; Schmutzler, Rita K.; Brauch, Hiltrud; Hamann, Ute; Ko, Yon-Dschun; Wang-Gohrke, Shan; Doerk, Thilo; Schuermann, Peter; Karstens, Johann H.; Hillemanns, Peter; Nevanlinna, Heli; Heikkinen, Tuomas; Aittomaki, Kristiina; Blomqvist, Carl; Bogdanova, Natalia V.; Zalutsky, Iosif V.; Antonenkova, Natalia N.; Bermisheva, Marina; Prokovieva, Darya; Farahtdinova, Albina; Khusnutdinova, Elza; Lindblom, Annika; Margolin, Sara; Mannermaa, Arto; Kataja, Vesa; Kosma, Veli-Matti; Hartikainen, Jaana; Chen, Xiaoqing; Beesley, Jonathan; Lambrechts, Diether; Zhao, Hui; Neven, Patrick; Wildiers, Hans; Nickels, Stefan; Flesch-Janys, Dieter; Radice, Paolo; Peterlongo, Paolo; Manoukian, Siranoush; Barile, Monica; Couch, Fergus J.; Olson, Janet E.; Wang, Xianshu; Fredericksen, Zachary; Giles, Graham G.; Baglietto, Laura; McLean, Catriona A.; Severi, Gianluca; Offit, Kenneth; Robson, Mark; Gaudet, Mia M.; Vijai, Joseph; Alnaes, Grethe Grenaker; Kristensen, Vessela; Borresen-Dale, Anne-Lise; John, Esther M.; Miron, Alexander; Winqvist, Robert; Pylkas, Katri; Jukkola-Vuorinen, Arja; Grip, Mervi; Andrulis, Irene L.; Knight, Julia A.; Glendon, Gord; Mulligan, Anna Marie; Figueroa, Jonine D.; Garcia-Closas, Montserrat; Lissowska, Jolanta; Sherman, Mark E.; Hooning, Maartje; Martens, John W. M.; Seynaeve, Caroline; Collee, Margriet; Hall, Per; Humpreys, Keith; Czene, Kamila; Liu, Jianjun; Cox, Angela; Brock, Ian W.; Cross, Simon S.; Reed, Malcolm W. R.; Ahmed, Shahana; Ghoussaini, Maya; Pharoah, Paul D. P.; Kang, Daehee; Yoo, Keun-Young; Noh, Dong-Young; Jakubowska, Anna; Jaworska, Katarzyna; Durda, Katarzyna; Zlowocka, Elzbieta; Sangrajrang, Suleeporn; Gaborieau, Valerie; Brennan, Paul; McKay, James; Shen, Chen-Yang; Yu, Jyh-Cherng; Hsu, Huan-Ming; Hou, Ming-Feng; Orr, Nick; Schoemaker, Minouk; Ashworth, Alan; Swerdlow, Anthony; Trentham-Dietz, Amy; Newcomb, Polly A.; Titus, Linda; Egan, Kathleen M.; Chenevix-Trench, Georgia; Antoniou, Antonis C.; Humphreys, Manjeet K.; Morrison, Jonathan; Chang-Claude, Jenny; Easton, Douglas F.; Dunning, Alison M.

    2012-01-01

    The 6q25.1 locus was first identified via a genome-wide association study (GWAS) in Chinese women and marked by single nucleotide polymorphism (SNP) rs2046210, approximately 180 Kb upstream of ESR1. There have been conflicting reports about the association of this locus with breast cancer in Europea

  20. Rose Atoll Site 27P 2/21/2012 2-3M

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — One-meter-square (1 meter x 1 meter) benthic substrate at Rose Atoll, site 27P (14 33.038S, 168 09.251W), between 2 and 3 meters along a permanent transect.

  1. 76 FR 73647 - National Healthy Worksite Program; Information Webinar Series

    Science.gov (United States)

    2011-11-29

    ... marketing campaign to reach employers including advertising in trade publications, direct mail/email, utilizing the Web site, producing a recruitment video and using social media. All participants must complete... full-time employees; medium--101-250 employees; and large--251-1000 employees). Outreach to...

  2. Dicty_cDB: Contig-U01814-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available one CH251-337M11 from chrom... 48 0.64 1 ( CT841554 ) Pig DNA sequence from clone CH242-267N7 on chromo... 48 0.64 1 ( AJ831604 ) Jor...danita hector mitochondrial tRNA-Leu (CUN) gen... 48 0.64 1 ( AC110798 ) Homo sapie

  3. 77 FR 63358 - Proposed Collection; Comment Request

    Science.gov (United States)

    2012-10-16

    ... the following two forms. RRB Form G-251a, Employer Job Information--Job Description, is released to an....) It is accompanied by a ``generic job description'' for that particular railroad job. The generic job... opportunity to comment on whether the job description matches the employee's actual duties. If the...

  4. 41 CFR 101-25.104-1 - Redistribution, repair, or rehabilitation.

    Science.gov (United States)

    2010-07-01

    ..., or rehabilitation. 101-25.104-1 Section 101-25.104-1 Public Contracts and Property Management Federal...-GENERAL 25.1-General Policies § 101-25.104-1 Redistribution, repair, or rehabilitation. Prior to the... through redistribution, repair, or rehabilitation of already owned furniture and office machines....

  5. Dicty_cDB: Contig-U14247-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available one CH251-505P8 from chromo... 44 7.5 1 ( EU081054 ) Carlowrightia torreyana trnS...-trnG intergenic spac... 44 7.5 1 ( EU081053 ) Carlowrightia texana trnS-trnG intergenic spacer,... 44 7.5 1 ( EU081052 ) Carlow

  6. The Influence of Self-Compassion on Academic Procrastination and Dysfunctional Attitudes

    Science.gov (United States)

    Iskender, Murat

    2011-01-01

    In the present study, aims were (1) to determine gender differences in self-compassion, academic procrastination, and dysfunctional attitudes and (2) to examine the relationships between self-compassion, academic procrastination, and dysfunctional attitudes. Participants were 251 university students who completed a questionnaire package that…

  7. Complete Genome Sequence of the Larval Shellfish Pathogen Vibrio tubiashii Type Strain ATCC 19109.

    Science.gov (United States)

    Richards, Gary P; Needleman, David S; Watson, Michael A; Bono, James L

    2014-12-18

    Vibrio tubiashii is a larval shellfish pathogen. Here, we report the first closed genome sequence for this species (ATCC type strain 19109), which consists of two chromosomes (3,294,490 and 1,766,582 bp), two megaplasmids (251,408 and 122,808 bp), and two plasmids (57,076 and 47,973 bp).

  8. The Apis mellifera filamentous virus genome

    Science.gov (United States)

    A complete reference genome of the Apis mellifera Filamentous virus (AmFV) was determined using Illumina Hiseq sequencing. The AmFV genome is a double strand DNA molecule of approximately 498’500 nucleotides with a GC content of 50.8%. It encompasses 251 non overlapping open reading frames (ORFs), e...

  9. Report: Low Frequency Predictive Skill Despite Structural Instability and Model Error

    Science.gov (United States)

    2013-09-30

    Structural Instability and Model Error Andrew J. Majda New York University Courant Institute of Mathematical Sciences 251 Mercer Street New York, NY...NUMBER 5e. TASK NUMBER 5f. WORK UNIT NUMBER 7. PERFORMING ORGANIZATION NAME(S) AND ADDRESS(ES) New York University, Courant Institute of

  10. Revision of the Thaneroclerinae (Cleridae, Col.)

    NARCIS (Netherlands)

    Corporaal, J.B.

    1939-01-01

    Mr. E. A. CHAPIN (in “Classification of the Philippine Components of the Coleopterous family Cleridae”; Philipp. Jrn. Sc. Vol. 25, No. 2, 1924, p. 165— 251) was the first to recognize the Thaneroclerinae as a distinct subfamily of the Cleridae, characterized chiefly by the broadly dilated anterior t

  11. Dicty_cDB: SHF189 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available s of Diseases Associated with Cell Cycle. 46 2e-04 3 BD452918 |BD452918.1 Diagnosis of Diseases Associated w...is of Diseases Associated with Cell Cycle. 46 2e-04 3 AX795908 |AX795908.1 Sequence 251 from Patent WO030521

  12. Mathematics Self-Efficacy: Stereotype Threat versus Resilience

    Science.gov (United States)

    Schweinle, Amy; Mims, Grace A.

    2009-01-01

    Children's academic self-efficacy is one of the strongest predictors of achievement (Wigfield and Eccles, "Contemporary Educational Psychology" 25(1): 68-81, 2000). The present research examined mathematics self-efficacy and the relationship of racial context from the perspective of two competing bodies of research. Stereotype threat theory would…

  13. 19 CFR 141.102 - When deposit of estimated duties, estimated taxes, or both not required.

    Science.gov (United States)

    2010-04-01

    ... withdrawal for consumption in the following situations may be made without depositing the estimated Customs... or manufacturer may enter or withdraw for consumption cigars, cigarettes, and cigarette papers and... regulations of the Bureau of Alcohol, Tobacco and Firearms (27 CFR part 251). (c) Deferral of payment of...

  14. Morlet Wavelet Analysis of ML >= 3 Earthquakes in the Taipei Metropolitan Area

    Directory of Open Access Journals (Sweden)

    Kou-Cheng Chen

    2015-01-01

    Full Text Available ML >= 3 earthquakes (ML = local magnitude that occurred in the Taipei Metropolitan Area (TMA from 1973 - 2013 are selected to study the dominant seismicity period of this area. The epicentral distribution and temporal sequences of earthquake magnitudes are simply described. These earthquakes can be divided into two groups: one for events shallower than 40 km and one for events deeper than 60 km. Shallow earthquakes are located mainly in the 0 - 10 km depth range north of 25.1°N,25.1°N, and down to 35 km for those south of 25.1°N.25.1°N. Deep events are located in the subduction zone, with a dip angle of about 70°.70°. The Morlet wavelet technique is applied to analyze the dominant periods of temporal variations in numbers of monthly earthquakes in the shallow and deep ranges for three magnitude ranges, i.e., ML >= 3, 4, and 5. The results show that for shallow earthquakes the dominant periods are 15.4, 30.8, 66.1, and 132.2 months when ML >= 3 and 30.8 months when ML >= 4; while for deep earthquakes, the dominant periods are 16.5 and 141.7 months when ML >= 3 and 141.7 months when ML >= 4. The dominant period cannot be obtained for both shallow and deep ML >= 5 earthquakes.

  15. Trans-ancestry genome-wide association study identifies 12 genetic loci influencing blood pressure and implicates a role for DNA methylation

    NARCIS (Netherlands)

    Kato, Norihiro; Loh, Marie; Takeuchi, Fumihiko; Verweij, Niek; Wang, Xu; Zhang, Weihua; Kelly, Tanika N.; Saleheen, Danish; Lehne, Benjamin; Leach, Irene Mateo; Drong, Alexander W.; Abbott, James; Wahl, Simone; Tan, Sian-Tsung; Scott, William R.; Campanella, Gianluca; Chadeau-Hyam, Marc; Afzal, Uzma; Ahluwalia, Tarunveer S.; Bonder, Marc Jan; Chen, Peng; Dehghan, Abbas; Edwards, Todd L.; Esko, Tonu; Go, Min Jin; Harris, Sarah E.; Hartiala, Jaana; Kasela, Silva; Kasturiratne, Anuradhani; Khor, Chiea-Chuen; Kleber, Marcus E.; Li, Huaixing; Mok, Zuan Yu; Nakatochi, Masahiro; Sapari, Nur Sabrina; Saxena, Richa; Stewart, Alexandre F. R.; Stolk, Lisette; Tabara, Yasuharu; Teh, Ai Ling; Wu, Ying; Wu, Jer-Yuarn; Zhang, Yi; Aits, Imke; Alves, Alexessander Da Silva Couto; Das, Shikta; Dorajoo, Rajkumar; Hopewell, Jemma C.; Kim, Yun Kyoung; Koivula, Robert W.; Luan, Jian'an; Lyytikainen, Leo-Pekka; Nguyen, Quang N.; Pereira, Mark A.; Postmus, Iris; Raitakari, Olli T.; Bryan, Molly Scannell; Scott, Robert A.; Sorice, Rossella; Tragante, Vinicius; Traglia, Michela; White, Jon; Yamamoto, Ken; Zhang, Yonghong; Adair, Linda S.; Ahmed, Alauddin; Akiyama, Koichi; Asif, Rasheed; Aung, Tin; Barroso, Ines; Bjonnes, Andrew; Braun, Timothy R.; Cai, Hui; Chang, Li-Ching; Chen, Chien-Hsiun; Cheng, Ching-Yu; Chong, Yap-Seng; Collins, Rory; Courtney, Regina; Davies, Gail; Delgado, Graciela; Do, Loi D.; Doevendans, Pieter A.; Gansevoort, Ron T.; Gao, Yu-Tang; Grammer, Tanja B.; Grarup, Niels; Grewal, Jagvir; Gu, Dongfeng; Wander, Gurpreet S.; Hartikainen, Anna-Liisa; Hazen, Stanley L.; He, Jing; Heng, Chew-Kiat; Hixson, James E.; Hofman, Albert; Hsu, Chris; Huang, Wei; Husemoen, Lise L. N.; Hwang, Joo-Yeon; Ichihara, Sahoko; Igase, Michiya; Isono, Masato; Justesen, Johanne M.; Katsuy, Tomohiro; Kibriya, Muhammad G.; Kim, Young Jin; Kishimoto, Miyako; Koh, Woon-Puay; Kohara, Katsuhiko; Kumari, Meena; Kwek, Kenneth; Lee, Nanette R.; Lee, Jeannette; Liao, Jiemin; Lieb, Wolfgang; Liewald, David C. M.; Matsubara, Tatsuaki; Matsushita, Yumi; Meitinger, Thomas; Mihailov, Evelin; Milani, Lili; Mills, Rebecca; Mononen, Nina; Mueller-Nurasyid, Martina; Nabika, Toru; Nakashima, Eitaro; Ng, Hong Kiat; Nikus, Kjell; Nutile, Teresa; Ohkubo, Takayoshi; Ohnaka, Keizo; Parish, Sarah; Paternoster, Lavinia; Peng, Hao; Peters, Annette; Pham, Son T.; Pinidiyapathirage, Mohitha J.; Rahman, Mahfuzar; Rakugi, Hiromi; Rolandsson, Olov; Rozario, Michelle Ann; Ruggiero, Daniela; Sala, Cinzia F.; Sarju, Ralhan; Shimokawa, Kazuro; Snieder, Harold; Sparso, Thomas; Spiering, Wilko; Starr, John M.; Stott, David J.; Stram, Daniel O.; Sugiyama, Takao; Szymczak, Silke; Tang, W. H. Wilson; Tong, Lin; Trompet, Stella; Turjanmaa, Vaino; Ueshima, Hirotsugu; Uitterlinden, Andre G.; Umemura, Satoshi; Vaarasmaki, Marja; van Dam, Rob M.; van Gilst, Wiek H.; van Veldhuisen, Dirk J.; Viikari, Jorma S.; Waldenberger, Melanie; Wang, Yiqin; Wang, Aili; Wilson, Rory; Wong, Tien-Yin; Xiang, Yong-Bing; Yamaguchi, Shuhei; Ye, Xingwang; Young, Robin D.; Young, Terri L.; Yuan, Jian-Min; Zhou, Xueya; Asselbergs, Folkert W.; Ciullo, Marina; Clarke, Robert; Deloukas, Panos; Franke, Andre; Franks, Paul W.; Franks, Steve; Friedlander, Yechiel; Gross, Myron D.; Guo, Zhirong; Hansen, Torben; Jarvelin, Marjo-Riitta; Jorgensen, Torben; Jukema, J. Wouter; Kahonen, Mika; Kajio, Hiroshi; Kivimaki, Mika; Lee, Jong-Young; Lehtimaki, Terho; Linneberg, Allan; Miki, Tetsuro; Pedersen, Oluf; Samani, Nilesh J.; Sorensen, Thorkild I. A.; Takayanagi, Ryoichi; Toniolo, Daniela; Ahsan, Habibul; Allayee, Hooman; Chen, Yuan-Tsong; Danesh, John; Deary, Ian J.; Franco, Oscar H.; Franke, Lude; Heijman, Bastiaan T.; Holbrook, Joanna D.; Isaacs, Aaron; Kim, Bong-Jo; Lin, Xu; Liu, Jianjun; Maerz, Winfried; Metspalu, Andres; Mohlke, Karen L.; Sanghera, Dharambir K.; Shu, Xiao-Ou; van Meurs, Joyce B. J.; Vithana, Eranga; Wickremasinghe, Ananda R.; Wijmenga, Cisca; Wolffenbuttel, Bruce H. W.; Yokota, Mitsuhiro; Zheng, Wei; Zhu, Dingliang; Vineis, Paolo; Kyrtopoulos, Soterios A.; Kleinjans, Jos C. S.; McCarthy, Mark I.; Soong, Richie; Gieger, Christian; Scott, James; Teo, Yik-Ying; He, Jiang; Elliott, Paul; Tai, E. Shyong; van der Harst, Pim; Kooner, Jaspal S.; Chambers, John C.; Doevendans, PAFM

    2015-01-01

    We carried out a trans-ancestry genome-wide association and replication study of blood pressure phenotypes among up to 320,251 individuals of East Asian, European and South Asian ancestry. We find genetic variants at 12 new loci to be associated with blood pressure (P = 3.9 x 10(-11) to 5.0 x 10(-21

  16. 7 CFR 1717.852 - Financing purposes.

    Science.gov (United States)

    2010-01-01

    ... and other transportation systems that supply fuel for generation, programs of demand side management..., except: (i) Certain load-management equipment (see 7 CFR 1710.251(c)); (ii) Renewable energy systems and RUS-approved programs of demand side management and energy conservation; and (iii) As determined...

  17. Technology, Opportunity & Access: Understanding Financial Inclusion in the U.S.

    OpenAIRE

    Nathaniel Karp; Boyd Nash-Stacey

    2015-01-01

    Although the U.S. is regarded as having a well-developed and deep financial system, financial inclusion continues to be a challenge for many communities and households. Using four databases with over 4 million data points and a PCA methodology, we developed the FIMI for 251 Metropolitan Statistical Areas, to help us identify the main determinants behind financial inclusion

  18. Collection Management, 1986.

    Science.gov (United States)

    Wortman, William A.

    1987-01-01

    The 1986 literature on collection management is reviewed under six major headings: (1) general overview; (2) materials; (3) kinds of users and libraries; (4) evaluation and cooperation; (5) selection, acquisitions, and allocation; and (6) access, weeding, and preservation. A list of 251 references is provided. (MES)

  19. Data of evolutionary structure change: 1DJ2B-1ADIB [Confc[Archive

    Lifescience Database Archive (English)

    Full Text Available 1DJ2B-1ADIB 1DJ2 1ADI B B IGSLSQVSGVLGCQWGDEGKGKLVDILAQHFDIVARCQG...LLSEACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAEKLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADI...LY CA 195 PHE CA 263 LYS CA 227 TYR CA 251 1ADI B 1ADIB 1 1ADI B 1ADI

  20. School of Advanced Military Studies Research Catalog, AY 1983 - 1984 Through AY 1993 - 1994

    Science.gov (United States)

    1994-01-01

    Every Clime and 258 098 Place: USMC Cold Weather Training Doc- trine ADA 251 427 Major Kevin Anastas , Information Overflow: Tactical Information...Major John E. Sterling. Obstacle "Theory": Do We Need It? ADA 258 249 Major Kevin Anastas , The American Way of Operational Art: Attrition or Maneuver