WorldWideScience

Sample records for berkelium 251

  1. New berkelium isotope: 242Bk

    International Nuclear Information System (INIS)

    A new isotope of berkelium, 242Bk, was produced with a cross section of approx. 10 μb in reactions of boron on uranium and nitrogen on thorium. It decays by electron capture with a half-life of 7 +- 1.3 minutes. The branching ratio for this isotope for alpha decay is less than 1% and that for spontaneous fission is less than 0.03%. 2 figures, 2 tables

  2. Characterization of berkelium(III) dipicolinate and borate compounds in solution and the solid state.

    Science.gov (United States)

    Silver, Mark A; Cary, Samantha K; Johnson, Jason A; Baumbach, Ryan E; Arico, Alexandra A; Luckey, Morgan; Urban, Matthew; Wang, Jamie C; Polinski, Matthew J; Chemey, Alexander; Liu, Guokui; Chen, Kuan-Wen; Van Cleve, Shelley M; Marsh, Matthew L; Eaton, Teresa M; van de Burgt, Lambertus J; Gray, Ashley L; Hobart, David E; Hanson, Kenneth; Maron, Laurent; Gendron, Frédéric; Autschbach, Jochen; Speldrich, Manfred; Kögerler, Paul; Yang, Ping; Braley, Jenifer; Albrecht-Schmitt, Thomas E

    2016-08-26

    Berkelium is positioned at a crucial location in the actinide series between the inherently stable half-filled 5f(7) configuration of curium and the abrupt transition in chemical behavior created by the onset of a metastable divalent state that starts at californium. However, the mere 320-day half-life of berkelium's only available isotope, (249)Bk, has hindered in-depth studies of the element's coordination chemistry. Herein, we report the synthesis and detailed solid-state and solution-phase characterization of a berkelium coordination complex, Bk(III)tris(dipicolinate), as well as a chemically distinct Bk(III) borate material for comparison. We demonstrate that berkelium's complexation is analogous to that of californium. However, from a range of spectroscopic techniques and quantum mechanical calculations, it is clear that spin-orbit coupling contributes significantly to berkelium's multiconfigurational ground state. PMID:27563098

  3. Extraction of tetravalent berkelium and cerium by aliquate-336-S-NO3 quaternary ammonium salt

    International Nuclear Information System (INIS)

    Extraction of tetravalent berkelium and cerium by aliquate-336-S-NO3 quaternary ammonium salt from nitric acid solutions is investigated. The effect of concentrations of nitric acid and extracting agent, nature of an oxidant (potassium bromate, potassium bichromate, mixture of AgNO3 and (NH4)2S2O8) and solvent on the distribution coefficient of berkelium(4) and cerium(4) is studied. It is established that solutions of aliquate-336-S-NO3 in carbon tetrachloride and dichloroethane extract quantitatively tetravalent berkelium from 10-12 M nitric acid solutions and cerium - from 1-10 M nitric acid solutions containing potassium bichromate as an oxidant. It is shown that the value of distribution coefficient for berkelium and cerium depends on the nature of an oxidant and extracting agent concentration. It is established that in the case of extraction by quaternary ammonium salt with one berkelium(4) mole four aliquate-336-SNO3 moles are associated and 1.5-1.6 mole of extracting agent are associated with one cerium(4) mole. It permits to make a conclusion that stoichiometry of extraction reactions by quaternary ammonium salt is not the same for tetravalent berkelium and cerium. It is shown that trivalent transplutonium and rare earth elements are not practically extracted by aliquate-336-S-NO3 from nitric acid solutions

  4. 25 CFR 700.251 - Fees.

    Science.gov (United States)

    2010-04-01

    ... 25 Indians 2 2010-04-01 2010-04-01 false Fees. 700.251 Section 700.251 Indians THE OFFICE OF... § 700.251 Fees. (a) Services for which fees may be charged. (1) Unless waived pursuant to the provisions of paragraph (c) of this section, user fees shall be charged for document search and...

  5. 37 CFR 1.251 - Unlocatable file.

    Science.gov (United States)

    2010-07-01

    ... 37 Patents, Trademarks, and Copyrights 1 2010-07-01 2010-07-01 false Unlocatable file. 1.251 Section 1.251 Patents, Trademarks, and Copyrights UNITED STATES PATENT AND TRADEMARK OFFICE, DEPARTMENT OF... § 1.251 Unlocatable file. (a) In the event that the Office cannot locate the file of an...

  6. 5 CFR 251.101 - Introduction.

    Science.gov (United States)

    2010-01-01

    ... 5 Administrative Personnel 1 2010-01-01 2010-01-01 false Introduction. 251.101 Section 251.101... ORGANIZATIONS REPRESENTING FEDERAL EMPLOYEES AND OTHER ORGANIZATIONS General Provisions § 251.101 Introduction... management officials and/or supervisors to the extent permitted by law. Under section 7(d)(2) and (3)...

  7. 14 CFR 29.251 - Vibration.

    Science.gov (United States)

    2010-01-01

    ... 14 Aeronautics and Space 1 2010-01-01 2010-01-01 false Vibration. 29.251 Section 29.251... STANDARDS: TRANSPORT CATEGORY ROTORCRAFT Flight Miscellaneous Flight Requirements § 29.251 Vibration. Each part of the rotorcraft must be free from excessive vibration under each appropriate speed and...

  8. 14 CFR 27.251 - Vibration.

    Science.gov (United States)

    2010-01-01

    ... 14 Aeronautics and Space 1 2010-01-01 2010-01-01 false Vibration. 27.251 Section 27.251... STANDARDS: NORMAL CATEGORY ROTORCRAFT Flight Miscellaneous Flight Requirements § 27.251 Vibration. Each part of the rotorcraft must be free from excessive vibration under each appropriate speed and...

  9. 7 CFR 251.7 - Formula adjustments.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 4 2010-01-01 2010-01-01 false Formula adjustments. 251.7 Section 251.7 Agriculture... GENERAL REGULATIONS AND POLICIES-FOOD DISTRIBUTION THE EMERGENCY FOOD ASSISTANCE PROGRAM § 251.7 Formula adjustments. Formula adjustments. (a) Commodity adjustments. The Department will make annual adjustments...

  10. 49 CFR 234.251 - Standby power.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 4 2010-10-01 2010-10-01 false Standby power. 234.251 Section 234.251 Transportation Other Regulations Relating to Transportation (Continued) FEDERAL RAILROAD ADMINISTRATION..., Inspection, and Testing Inspections and Tests § 234.251 Standby power. Standby power shall be tested at...

  11. 15 CFR 904.251 - Evidence.

    Science.gov (United States)

    2010-01-01

    ... 15 Commerce and Foreign Trade 3 2010-01-01 2010-01-01 false Evidence. 904.251 Section 904.251... and Appeal Procedures Hearings § 904.251 Evidence. (a) In general. (1) At the hearing, every party has the right to present oral or documentary evidence in support of its case or defense, to...

  12. 7 CFR 251.2 - Administration.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 4 2010-01-01 2010-01-01 false Administration. 251.2 Section 251.2 Agriculture... Administration. (a) Food and Nutrition Service. Within the United States Department of Agriculture (the... this section, any information on changes in program administration, including any changes...

  13. 40 CFR 436.251 - Specialized definitions.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 29 2010-07-01 2010-07-01 false Specialized definitions. 436.251 Section 436.251 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS MINERAL MINING AND PROCESSING POINT SOURCE CATEGORY Jade Subcategory §...

  14. 37 CFR 251.43 - Written cases.

    Science.gov (United States)

    2010-07-01

    ... 251.43 Patents, Trademarks, and Copyrights COPYRIGHT OFFICE, LIBRARY OF CONGRESS COPYRIGHT ARBITRATION ROYALTY PANEL RULES AND PROCEDURES COPYRIGHT ARBITRATION ROYALTY PANEL RULES OF PROCEDURE Procedures of Copyright Arbitration Royalty Panels § 251.43 Written cases. (a) All parties who have filed a notice...

  15. 37 CFR 2.51 - Drawing required.

    Science.gov (United States)

    2010-07-01

    ... 37 Patents, Trademarks, and Copyrights 1 2010-07-01 2010-07-01 false Drawing required. 2.51... COMMERCE RULES OF PRACTICE IN TRADEMARK CASES Drawing § 2.51 Drawing required. (a) In an application under section 1(a) of the Act, the drawing of the mark must be a substantially exact representation of the...

  16. 47 CFR 0.251 - Authority delegated.

    Science.gov (United States)

    2010-10-01

    ..., 309, 315, 317) Editorial Note: For Federal Register citations affecting § 0.251, see the List of CFR... Collection Improvement Act of 1996, Public Law 104-134, 110 Stat. 1321, 1358 (1996) (DCIA), including,...

  17. 36 CFR 251.97 - Oral presentation.

    Science.gov (United States)

    2010-07-01

    ... 36 Parks, Forests, and Public Property 2 2010-07-01 2010-07-01 false Oral presentation. 251.97... presentation. (a) Purpose. An oral presentation provides an additional opportunity for an appellant, and other..., emphasize, and/or clarify information related to an appeal. Oral presentations are to be conducted in...

  18. 27 CFR 25.251 - Authorized removals.

    Science.gov (United States)

    2010-04-01

    ..., DEPARTMENT OF THE TREASURY LIQUORS BEER Removal of Brewer's Yeast and Other Articles § 25.251 Authorized removals. (a) Brewer's yeast. A brewer may remove brewer's yeast, in liquid or solid form containing not... articles. A brewer may remove malt, malt syrup, wort, and other articles from the brewery. (e) Methods...

  19. 6 CFR 25.1 - Purpose.

    Science.gov (United States)

    2010-01-01

    ... DEPARTMENT OF HOMELAND SECURITY, OFFICE OF THE SECRETARY REGULATIONS TO SUPPORT ANTI-TERRORISM BY FOSTERING EFFECTIVE TECHNOLOGIES § 25.1 Purpose. This part implements the Support Anti-terrorism by Fostering Effective Technologies Act of 2002, sections 441-444 of title 6, United States Code (the “SAFETY Act”...

  20. 36 CFR 251.55 - Nature of interest.

    Science.gov (United States)

    2010-07-01

    ... 36 Parks, Forests, and Public Property 2 2010-07-01 2010-07-01 false Nature of interest. 251.55 Section 251.55 Parks, Forests, and Public Property FOREST SERVICE, DEPARTMENT OF AGRICULTURE LAND USES Special Uses § 251.55 Nature of interest. (a) A holder is authorized only to occupy such land...

  1. 27 CFR 25.1 - Production and removal of beer.

    Science.gov (United States)

    2010-04-01

    ... beer. 25.1 Section 25.1 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE BUREAU, DEPARTMENT OF THE TREASURY LIQUORS BEER Scope of Regulations § 25.1 Production and removal of beer. The regulations in this part relate to beer and cereal beverages and cover the location, construction,...

  2. 7 CFR 251.4 - Availability of commodities.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 4 2010-01-01 2010-01-01 false Availability of commodities. 251.4 Section 251.4... Availability of commodities. (a) General. The Department shall make commodities available for distribution and... receiving commodities under this part shall not diminish their normal expenditures for food because...

  3. 24 CFR 221.251 - Cross-reference.

    Science.gov (United States)

    2010-04-01

    ... DEVELOPMENT MORTGAGE AND LOAN INSURANCE PROGRAMS UNDER NATIONAL HOUSING ACT AND OTHER AUTHORITIES LOW COST AND MODERATE INCOME MORTGAGE INSURANCE-SAVINGS CLAUSE Contract Rights and Obligations-Low Cost Homes § 221.251... 24 Housing and Urban Development 2 2010-04-01 2010-04-01 false Cross-reference. 221.251...

  4. 14 CFR 21.251 - Limits of applicability.

    Science.gov (United States)

    2010-01-01

    ... 14 Aeronautics and Space 1 2010-01-01 2010-01-01 false Limits of applicability. 21.251 Section 21.251 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION AIRCRAFT... for which the manufacturer holds, or obtains, a type certificate; (3) The amendment of a...

  5. 37 CFR 251.12 - Conduct of open meetings.

    Science.gov (United States)

    2010-07-01

    ... Access to Copyright Arbitration Royalty Panel Meetings § 251.12 Conduct of open meetings. Meetings of a....12 Section 251.12 Patents, Trademarks, and Copyrights COPYRIGHT OFFICE, LIBRARY OF CONGRESS COPYRIGHT... possible. Reasonable access for the public will be provided at all public sessions. Any person may...

  6. Dicty_cDB: VHG251 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available VH (Link to library) VHG251 (Link to dictyBase) - - - Contig-U16430-1 VHG251P (Link to Original ... mRNA f... 87 4e-16 DQ383787_1( DQ383787 |pid:none) Rhodomonas ... salina 82 kDa heat shoc... 86 5e-16 AF165818_7( AF ...

  7. 37 CFR 251.15 - Transcripts of closed meetings.

    Science.gov (United States)

    2010-07-01

    ... case of § 251.13(h) and at the discretion of the Copyright Arbitration Royalty Panel, detailed minutes... COPYRIGHT ARBITRATION ROYALTY PANEL RULES AND PROCEDURES COPYRIGHT ARBITRATION ROYALTY PANEL RULES OF PROCEDURE Public Access to Copyright Arbitration Royalty Panel Meetings § 251.15 Transcripts of...

  8. 36 CFR 251.59 - Transfer of authorized improvements.

    Science.gov (United States)

    2010-07-01

    ..., voluntary sale, transfer, or through enforcement of a valid legal proceeding or operation of law, ceases to... 36 Parks, Forests, and Public Property 2 2010-07-01 2010-07-01 false Transfer of authorized improvements. 251.59 Section 251.59 Parks, Forests, and Public Property FOREST SERVICE, DEPARTMENT...

  9. 37 CFR 251.30 - Basic obligations of arbitrators.

    Science.gov (United States)

    2010-07-01

    ... arbitrators. 251.30 Section 251.30 Patents, Trademarks, and Copyrights COPYRIGHT OFFICE, LIBRARY OF CONGRESS COPYRIGHT ARBITRATION ROYALTY PANEL RULES AND PROCEDURES COPYRIGHT ARBITRATION ROYALTY PANEL RULES OF... they are violating the law or the ethical standards set forth in this subpart. (9) Arbitrators...

  10. Protein engineering of cyclodextrin glycosyltransferase from Bacillus circulans strain 251

    OpenAIRE

    Penninga, Dirk

    1996-01-01

    An enormous diversity of molecular functions in living organisms is carried out by proteins. Our studies have focussed on the functional analysis of a starch-converting enzyme, cyclodextrin glycosyltransferase (CGTase) from Bacillus circulans strain 251. Zie: Summary

  11. Dicty_cDB: SFK251 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available SF (Link to library) SFK251 (Link to dictyBase) - - - Contig-U16397-1 SFK251F (Link to Original ... 03344 ) Dictyostelium discoideum mRNA for cysteine proteina ... 174 6e-40 1 ( M16039 ) Dictyostelium discoideum ... ts: (bits) Value ( P04989 ) RecName: Full=Cysteine proteina se 2; EC=3.4.22... 65 9e-10 protein update 2009. 1. ...

  12. 36 CFR 251.56 - Terms and conditions.

    Science.gov (United States)

    2010-07-01

    ... plan governing the area (36 CFR part 219). (c) Preconstruction approvals. Forest Service approval of... Special Uses § 251.56 Terms and conditions. (a) General. (1) Each special use authorization must contain... and economic interests; (B) Manage efficiently the lands subject to the use and adjacent thereto;...

  13. 27 CFR 28.251 - Railway express receipts.

    Science.gov (United States)

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2010-04-01 2010-04-01 false Railway express receipts... § 28.251 Railway express receipts. Where the exportation is to a contiguous foreign country and the shipment is by railway express, a receipt issued by the railway express agency may be accepted in lieu...

  14. 28 CFR 25.1 - Purpose and authority.

    Science.gov (United States)

    2010-07-01

    ... Instant Criminal Background Check System § 25.1 Purpose and authority. The purpose of this subpart is to... Instant Criminal Background Check System (NICS) to be contacted by any licensed importer, licensed... procedures for persons who have been denied the right to obtain a firearm as a result of a NICS...

  15. 37 CFR 251.32 - Financial disclosure statement.

    Science.gov (United States)

    2010-07-01

    ... nomination, each nominated arbitrator shall file with the Librarian of Congress a confidential financial... Librarian and designated Library staff to determine what conflicts of interest, if any, exist according to § 251.31. (b) If any conflicts do exist, the Librarian shall not choose that person for the...

  16. 48 CFR 2452.251-70 - Contractor employee travel.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 6 2010-10-01 2010-10-01 true Contractor employee travel... 2452.251-70 Contractor employee travel. As prescribed in 2451.7001, insert the following clause in all cost-reimbursement solicitations and contracts involving travel: Contractor Employee Travel (OCT...

  17. 37 CFR 251.62 - Content of petition.

    Science.gov (United States)

    2010-07-01

    ... ARBITRATION ROYALTY PANEL RULES AND PROCEDURES COPYRIGHT ARBITRATION ROYALTY PANEL RULES OF PROCEDURE Rate Adjustment Proceedings § 251.62 Content of petition. (a) In the case of a petition for rate adjustment... similar interests may file a petition jointly. (b) In the case of a petition for rate...

  18. 37 CFR 251.71 - Commencement of proceedings.

    Science.gov (United States)

    2010-07-01

    ... COPYRIGHT ARBITRATION ROYALTY PANEL RULES AND PROCEDURES COPYRIGHT ARBITRATION ROYALTY PANEL RULES OF PROCEDURE Royalty Fee Distribution Proceedings § 251.71 Commencement of proceedings. (a) Cable. In the case... year in accordance with the requirements of this subchapter. (b) Satellite carriers. In the case...

  19. 37 CFR 251.45 - Discovery and prehearing motions.

    Science.gov (United States)

    2010-07-01

    ... COPYRIGHT ARBITRATION ROYALTY PANEL RULES AND PROCEDURES COPYRIGHT ARBITRATION ROYALTY PANEL RULES OF PROCEDURE Procedures of Copyright Arbitration Royalty Panels § 251.45 Discovery and prehearing motions. (a) Request for comment, notice of intention to participate. In the case of a royalty fee...

  20. 37 CFR 251.61 - Commencement of adjustment proceedings.

    Science.gov (United States)

    2010-07-01

    ... COPYRIGHT ARBITRATION ROYALTY PANEL RULES AND PROCEDURES COPYRIGHT ARBITRATION ROYALTY PANEL RULES OF PROCEDURE Rate Adjustment Proceedings § 251.61 Commencement of adjustment proceedings. (a) In the case of... programming exclusivity. (c) In the case of noncommercial educational broadcasting, a petition is...

  1. 37 CFR 251.4 - Arbitrator lists: Objections.

    Science.gov (United States)

    2010-07-01

    ... COPYRIGHT ARBITRATION ROYALTY PANEL RULES AND PROCEDURES COPYRIGHT ARBITRATION ROYALTY PANEL RULES OF PROCEDURE Organization § 251.4 Arbitrator lists: Objections. (a) In the case of a rate adjustment proceeding... be objectionable. (b) In the case of a royalty distribution proceeding, any party to the...

  2. 37 CFR 251.72 - Declaration of controversy: Initiation of arbitration proceeding.

    Science.gov (United States)

    2010-07-01

    ...: Initiation of arbitration proceeding. 251.72 Section 251.72 Patents, Trademarks, and Copyrights COPYRIGHT OFFICE, LIBRARY OF CONGRESS COPYRIGHT ARBITRATION ROYALTY PANEL RULES AND PROCEDURES COPYRIGHT ARBITRATION ROYALTY PANEL RULES OF PROCEDURE Royalty Fee Distribution Proceedings § 251.72 Declaration...

  3. 37 CFR 251.64 - Disposition of petition; initiation of arbitration proceeding.

    Science.gov (United States)

    2010-07-01

    ...; initiation of arbitration proceeding. 251.64 Section 251.64 Patents, Trademarks, and Copyrights COPYRIGHT OFFICE, LIBRARY OF CONGRESS COPYRIGHT ARBITRATION ROYALTY PANEL RULES AND PROCEDURES COPYRIGHT ARBITRATION ROYALTY PANEL RULES OF PROCEDURE Rate Adjustment Proceedings § 251.64 Disposition of...

  4. 37 CFR 251.2 - Purpose of Copyright Arbitration Royalty Panels.

    Science.gov (United States)

    2010-07-01

    ... Arbitration Royalty Panels. 251.2 Section 251.2 Patents, Trademarks, and Copyrights COPYRIGHT OFFICE, LIBRARY OF CONGRESS COPYRIGHT ARBITRATION ROYALTY PANEL RULES AND PROCEDURES COPYRIGHT ARBITRATION ROYALTY PANEL RULES OF PROCEDURE Organization § 251.2 Purpose of Copyright Arbitration Royalty Panels....

  5. 37 CFR 251.7 - Actions of Copyright Arbitration Royalty Panels.

    Science.gov (United States)

    2010-07-01

    ... Arbitration Royalty Panels. 251.7 Section 251.7 Patents, Trademarks, and Copyrights COPYRIGHT OFFICE, LIBRARY OF CONGRESS COPYRIGHT ARBITRATION ROYALTY PANEL RULES AND PROCEDURES COPYRIGHT ARBITRATION ROYALTY PANEL RULES OF PROCEDURE Organization § 251.7 Actions of Copyright Arbitration Royalty Panels....

  6. 37 CFR 251.54 - Assessment of costs of arbitration panels.

    Science.gov (United States)

    2010-07-01

    ... arbitration panels. 251.54 Section 251.54 Patents, Trademarks, and Copyrights COPYRIGHT OFFICE, LIBRARY OF CONGRESS COPYRIGHT ARBITRATION ROYALTY PANEL RULES AND PROCEDURES COPYRIGHT ARBITRATION ROYALTY PANEL RULES OF PROCEDURE Procedures of Copyright Arbitration Royalty Panels § 251.54 Assessment of costs...

  7. 37 CFR 251.6 - Composition and selection of Copyright Arbitration Royalty Panels.

    Science.gov (United States)

    2010-07-01

    ... Copyright Arbitration Royalty Panels. 251.6 Section 251.6 Patents, Trademarks, and Copyrights COPYRIGHT OFFICE, LIBRARY OF CONGRESS COPYRIGHT ARBITRATION ROYALTY PANEL RULES AND PROCEDURES COPYRIGHT ARBITRATION ROYALTY PANEL RULES OF PROCEDURE Organization § 251.6 Composition and selection of...

  8. 36 CFR 251.23 - Experimental areas and research natural areas.

    Science.gov (United States)

    2010-07-01

    ... 36 Parks, Forests, and Public Property 2 2010-07-01 2010-07-01 false Experimental areas and research natural areas. 251.23 Section 251.23 Parks, Forests, and Public Property FOREST SERVICE, DEPARTMENT OF AGRICULTURE LAND USES Miscellaneous Land Uses Designation of Areas § 251.23 Experimental...

  9. 46 CFR 251.11 - Applications under Title VI, Merchant Marine Act, 1936, as amended.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 8 2010-10-01 2010-10-01 false Applications under Title VI, Merchant Marine Act, 1936, as amended. 251.11 Section 251.11 Shipping MARITIME ADMINISTRATION, DEPARTMENT OF TRANSPORTATION... AID § 251.11 Applications under Title VI, Merchant Marine Act, 1936, as amended. (a)...

  10. Inhibition of X-rays irradiaiton combined with thalidomide on glioma U251 cells

    International Nuclear Information System (INIS)

    In order to investigate the inhibition effect of X-rays combined with thalidomide on glioma U251 cells, the cell scratch assay was used to detect the inhibition of thalidomide on U251 cells. And H3-TdR incorporation assay and colony formation were used to investigate the enhancement effect of thalidomide on the sensitivity of U251 cells to X-rays. It has been found that thalidomide has synergistic effect on therapy of glioma U251 cells if it is combined with X-rays irradiation as it can inhibit cells infiltration, DNA synthesis and colony formation. When the survival rate of glioma U251 cells is 50%, the radio-sensitization factors of 60 μg/mL and 100 μg/mL thalidomide is 1.18 and 1.51 respectively. The results reveal that thalidomide could significantly improve radio-sensitivity of glioma U251 cells. (authors)

  11. 7 CFR 2.51 - Deputy Under Secretary for Food Safety.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 1 2010-01-01 2010-01-01 false Deputy Under Secretary for Food Safety. 2.51 Section 2... Safety § 2.51 Deputy Under Secretary for Food Safety. Pursuant to § 2.18, and subject to policy guidance... Secretary for Food Safety to the Deputy Under Secretary for Food Safety, to be exercised only during...

  12. 37 CFR 251.36 - Pre-arbitration and post-arbitration employment restrictions.

    Science.gov (United States)

    2010-07-01

    ... 37 Patents, Trademarks, and Copyrights 1 2010-07-01 2010-07-01 false Pre-arbitration and post-arbitration employment restrictions. 251.36 Section 251.36 Patents, Trademarks, and Copyrights COPYRIGHT OFFICE, LIBRARY OF CONGRESS COPYRIGHT ARBITRATION ROYALTY PANEL RULES AND PROCEDURES...

  13. TWOS - TIME WARP OPERATING SYSTEM, VERSION 2.5.1

    Science.gov (United States)

    Bellenot, S. F.

    1994-01-01

    The Time Warp Operating System (TWOS) is a special-purpose operating system designed to support parallel discrete-event simulation. TWOS is a complete implementation of the Time Warp mechanism, a distributed protocol for virtual time synchronization based on process rollback and message annihilation. Version 2.5.1 supports simulations and other computations using both virtual time and dynamic load balancing; it does not support general time-sharing or multi-process jobs using conventional message synchronization and communication. The program utilizes the underlying operating system's resources. TWOS runs a single simulation at a time, executing it concurrently on as many processors of a distributed system as are allocated. The simulation needs only to be decomposed into objects (logical processes) that interact through time-stamped messages. TWOS provides transparent synchronization. The user does not have to add any more special logic to aid in synchronization, nor give any synchronization advice, nor even understand much about how the Time Warp mechanism works. The Time Warp Simulator (TWSIM) subdirectory contains a sequential simulation engine that is interface compatible with TWOS. This means that an application designer and programmer who wish to use TWOS can prototype code on TWSIM on a single processor and/or workstation before having to deal with the complexity of working on a distributed system. TWSIM also provides statistics about the application which may be helpful for determining the correctness of an application and for achieving good performance on TWOS. Version 2.5.1 has an updated interface that is not compatible with 2.0. The program's user manual assists the simulation programmer in the design, coding, and implementation of discrete-event simulations running on TWOS. The manual also includes a practical user's guide to the TWOS application benchmark, Colliding Pucks. TWOS supports simulations written in the C programming language. It is designed

  14. 8 CFR 251.3 - Departure manifests and lists for vessels.

    Science.gov (United States)

    2010-01-01

    ... vessels. (a) Form I-418, Passenger List-Crew List. The master or agent of every vessel departing from the... compliance with section 251(c) of the Act. (b) Exception for certain Great Lakes vessels. The required...

  15. 29 CFR 779.251 - Goods that have lost their out-of-State identity.

    Science.gov (United States)

    2010-07-01

    ... 29 Labor 3 2010-07-01 2010-07-01 false Goods that have lost their out-of-State identity. 779.251... Coverage Interstate Inflow Test Under Prior Act § 779.251 Goods that have lost their out-of-State identity... been processed or manufactured so as to have lost their identity as out-of-State goods before they...

  16. The biarylpyrazole compound AM251 alters mitochondrial physiology via proteolytic degradation of ERRα.

    Science.gov (United States)

    Krzysik-Walker, Susan M; González-Mariscal, Isabel; Scheibye-Knudsen, Morten; Indig, Fred E; Bernier, Michel

    2013-01-01

    The orphan nuclear receptor estrogen-related receptor alpha (ERRα) directs the transcription of nuclear genes involved in energy homeostasis control and the regulation of mitochondrial mass and function. A crucial role for controlling ERRα-mediated target gene expression has been ascribed to the biarylpyrazole compound 1-(2,4-dichlorophenyl)-5-(4-iodophenyl)-4-methyl-N-1-piperidinyl-1H-pyrazole-3-carboxamide (AM251) through direct binding to and destabilization of ERRα protein. Here, we provide evidence that structurally related AM251 analogs also have negative impacts on ERRα protein levels in a cell-type-dependent manner while having no deleterious actions on ERRγ. We show that these off-target cellular effects of AM251 are mediated by proteasomal degradation of nuclear ERRα. Cell treatment with the nuclear export inhibitor leptomycin B did not prevent AM251-induced destabilization of ERRα protein, whereas proteasome inhibition with MG132 stabilized and maintained its DNA-binding function, indicative of ERRα being a target of nuclear proteasomal complexes. NativePAGE analysis revealed that ERRα formed a ∼220-kDa multiprotein nuclear complex that was devoid of ERRγ and the coregulator peroxisome proliferator-activated receptor γ coactivator-1. AM251 induced SUMO-2,3 incorporation in ERRα in conjunction with increased protein kinase C activity, whose activation by phorbol ester also promoted ERRα protein loss. Down-regulation of ERRα by AM251 or small interfering RNA led to increased mitochondria biogenesis while negatively impacting mitochondrial membrane potential. These results reveal a novel molecular mechanism by which AM251 and related compounds alter mitochondrial physiology through destabilization of ERRα. PMID:23066093

  17. Radiosensitivity of brain cancer stem cells from malignant glicoma cell line U251 in vitro

    International Nuclear Information System (INIS)

    Objective: To investigate the radiosensitivity of brain cancer stem cells of different conditions isolated from malignant glioma cell line U251 irt vitro. Methods: The brain cancer stem cells in U251 or the brain cancer stem cells isolated from U251 were irradiated by 60Co γ-rays. TUNEL and Annexin-FITC were employed to detect the apoptosis. The brain cancer stem cells were subcutaneously transplanted to nude mouse. Flow cytometry was used to detect cell cycle. Results: The brain cancer stem cells isolated from malignant glioma cell line U251 were in active cell cycle and sensitive to 60Co γ-rays. Thed apoptotic cells were increased obviously after irradiation. After subcutaneously transplanted to unde mouse, there was no tumor appear. However; the brain cancer stem cells existed in U251 were in G0-G1 and resisted to 60Co γ-rays. They differentiated into the parent glioma type after traqnsplantation. Conclusions: The brain cancer stem cells existed in the malignant glioma cell line is resisted to irradiation, and this phenomenon may explain the glioma relapse irt situ after radiation therapy. (authors)

  18. Analysis of 251 cases of adverse drug reactions in our hospital report%我院251例药品不良反应报告分析

    Institute of Scientific and Technical Information of China (English)

    唐锦辉

    2013-01-01

      purpose: to understand adverse drug reactions in our hospital (Adverse Drug Reaction) the incidence and relevant factors, provide reference for rational drug use, avoid the occurrence of adverse drug reactions. Methods: 251 cases on our 2011 colection statistics, analysis of ADR reports. Results 251 cases ADR report total involved 16 class drugs, vein drops note is caused ADR of main to drug way (201 cases); antibiotics occurred rate Supreme (142 cases), second is proprietary Chinese medicines (28 cases), and antipyretic analgesia drug (20 cases), and anti-tumor drug (16 cases), and effect blood and hematopoietic system of drug (12 cases); clinical performance main for skin and damage (99 cases), accounted for 39.44%, digestive system of of damage (75 cases) accounted for 29.88%. Conclusion: ADR is related to many factors relevant to clinical ADR monitoring work should be strengthened to reduce or avoid the occurrence of ADR.

  19. Chronic administration of AM251 improves albuminuria and renal tubular structure in obese rats.

    Science.gov (United States)

    Jenkin, Kayte A; O'Keefe, Lannie; Simcocks, Anna C; Grinfeld, Esther; Mathai, Michael L; McAinch, Andrew J; Hryciw, Deanne H

    2015-05-01

    Modulation of the endocannabinoid system as an anti-obesity therapeutic is well established; however, the direct effects of cannabinoid receptor 1 (CB1) antagonism on renal function and structure in a model of diet-induced obesity (DIO) are unknown. The aim of this study was to characterise the renal effects of the CB1 antagonist AM251 in a model of DIO. Male Sprague-Dawley rats were fed a low- or high-fat diet (HFD: 40% digestible energy from lipids) for 10 weeks to elicit DIO (n=9). In a different cohort, rats were fed a HFD for 15 weeks. After 9 weeks consuming a HFD, rats were injected daily for 6 weeks with 3 mg/kg AM251 (n=9) or saline via i.p. injection (n=9). After 10 weeks consuming a HFD, CB1 and megalin protein expression were significantly increased in the kidneys of obese rats. Antagonism of CB1 with AM251 significantly reduced weight gain, systolic blood pressure, plasma leptin, and reduced albuminuria and plasma creatinine levels in obese rats. Importantly, there was a significant reduction in tubular cross-section diameter in the obese rats treated with AM251. An improvement in albuminuria was likely due to the reduction in tubular size, reduced leptinaemia and maintenance of megalin expression levels. In obese rats, AM251 did not alter diastolic blood pressure, sodium excretion, creatinine clearance or expression of the fibrotic proteins VEGFA, TGFB1 and collagen IV in the kidney. This study demonstrates that treatment with CB1 antagonist AM251 improves renal outcomes in obese rats. PMID:25804605

  20. U-251 revisited: genetic drift and phenotypic consequences of long-term cultures of glioblastoma cells

    International Nuclear Information System (INIS)

    It is well known that in vitro subculture represents a selection pressure on cell lines, and over time this may result in a genetic drift in the cancer cells. In addition, long-term cultures harbor the risk of cross-contamination with other cell lines. The consequences may have major impact on experimental results obtained in various laboratories, where the cell lines no longer reflect the original tumors that they are supposed to represent. Much neglected in the scientific community is a close monitoring of cell cultures by regular phenotypic and genetic characterization. In this report, we present a thorough characterization of the commonly used glioblastoma (GBM) model U-251, which in numerous publications has been wrongly identified as U-373, due to an earlier cross-contamination. In this work, the original U-251 and three subclones of U-251, commonly referred to as U-251 or U-373, were analyzed with regard to their DNA profile, morphology, phenotypic expression, and growth pattern. By array comparative genomic hybridization (aCGH), we show that only the original low-passaged U-251 cells, established in the 1960s, maintain a DNA copy number resembling a typical GBM profile, whereas all long-term subclones lost the typical GBM profile. Also the long-term passaged subclones displayed variations in phenotypic marker expression and showed an increased growth rate in vitro and a more aggressive growth in vivo. Taken together, the variations in genotype and phenotype as well as differences in growth characteristics may explain different results reported in various laboratories related to the U-251 cell line

  1. The prosurvival role of autophagy in Resveratrol-induced cytotoxicity in human U251 glioma cells

    International Nuclear Information System (INIS)

    Previous study reported that resveratrol has anti-tumor activity. In this study, we investigated the involvement of autophagy in the resveratrol-induced apoptotic death of human U251 glioma cells. The growth inhibition of U251 cells induced by resveratrol was assessed with methyl thiazolyl tetrazolium (MTT). The activation of autophagy and proapoptotic effect were characterized by monodansylcadaverine labeling and Hoechst stain, respectively. Mitochondrialtransmembrane potential (ΔΨm) was measured as a function of drug treatment using 5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolylcarbocyanine iodide (JC-1). The role of autophagy and apoptosis in the resveratrol-induced death of U251 cells was assessed using autophagic and caspase inhibitors. Immunofluorescence, flow cytometry, and Western blot analysis were used to study the apoptotic and autophagic mechanisms. Methyl thiazolyl tetrazolium (MTT) assays indicated that resveratrol decreased the viability of U251 cells in a dose- and time-dependent manner. Flow cytometry analysis indicated that resveratrol increased cell population at sub-G1 phase, an index of apoptosis. Furthermore, resveratrol-induced cell death was associated with a collapse of the mitochondrial membrane potential. The pan-caspase inhibitor Z-VAD-fmk suppressed resveratrol-induced U251 cell death. Resveratrol stimulated autophagy was evidenced by punctuate monodansylcadaverine(MDC) staining and microtubule-associated protein light chain 3 (LC3) immunoreactivty. Resveratrol also increased protein levels of beclin 1 and membrane form LC3 (LC3-II). Autophagy inhibitors 3-methylademine (3-MA) and bafilomycin A1 sensitized the cytotoxicity of resveratrol. Together, these findings indicate that resveratrol induces autophagy in human U251 glioma cells and autophagy suppressed resveratrol-induced apoptosis. This study thus suggests that autophagy inhibitors can increase the cytotoxicity of resveratrol to glioma cells

  2. 30 CFR 251.11 - Submission, inspection, and selection of geological data and information collected under a permit...

    Science.gov (United States)

    2010-07-01

    ... 30 CFR part 250, subpart N; and (2) A permittee or third party that sells, trades, licenses, or... geological data and information collected under a permit and processed by permittees or third parties. 251.11... GEOLOGICAL AND GEOPHYSICAL (G&G) EXPLORATIONS OF THE OUTER CONTINENTAL SHELF § 251.11 Submission,...

  3. 30 CFR 251.13 - Reimbursement for the costs of reproducing data and information and certain processing costs.

    Science.gov (United States)

    2010-07-01

    ... 30 Mineral Resources 2 2010-07-01 2010-07-01 false Reimbursement for the costs of reproducing data and information and certain processing costs. 251.13 Section 251.13 Mineral Resources MINERALS... certain processing costs. (a) MMS will reimburse you or a third party for reasonable costs of...

  4. 47 CFR 24.251 - Dispute resolution under the Cost-Sharing Plan.

    Science.gov (United States)

    2010-10-01

    ... 47 Telecommunication 2 2010-10-01 2010-10-01 false Dispute resolution under the Cost-Sharing Plan... 1850-1990 Mhz Band § 24.251 Dispute resolution under the Cost-Sharing Plan. Disputes arising out of the cost-sharing plan, such as disputes over the amount of reimbursement required, must be brought, in...

  5. 8 CFR 251.5 - Paper arrival and departure manifests for crew.

    Science.gov (United States)

    2010-01-01

    ... from the United States must submit arrival and departure manifests in a paper format in accordance with... 8 Aliens and Nationality 1 2010-01-01 2010-01-01 false Paper arrival and departure manifests for... REGULATIONS ARRIVAL AND DEPARTURE MANIFESTS AND LISTS: SUPPORTING DOCUMENTS § 251.5 Paper arrival...

  6. 7 CFR 1710.251 - Construction work plans-distribution borrowers.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 11 2010-01-01 2010-01-01 false Construction work plans-distribution borrowers. 1710... LOANS AND GUARANTEES Construction Work Plans and Related Studies § 1710.251 Construction work plans..., including the related costs of installation, is limited to capital equipment designed to influence the...

  7. Role of Autophagy in Capsaicin-Induced Apoptosis in U251 Glioma Cells.

    Science.gov (United States)

    Liu, Ya-Ping; Dong, Fu-Xing; Chai, Xiang; Zhu, Shuang; Zhang, Bao-Le; Gao, Dian-Shuai

    2016-07-01

    In recent years, the role of capsaicin in cancer prevention and treatment has gained people's attention. However, the mechanism of anti-glioma cells by capsaicin has not been elucidated. Here, we discuss the mechanism of capsaicin in U251 cells. Cell viability was detected by MTT and extracellular LDH measurements, while immunofluorescence was performed to measure changes of LC3 in U251 cells. The expressions of LC3II, Puma-α, Beclin1, P62, Procaspase-3, and P53 were observed by immunoblotting. The cell viability decreased and the punctate patterns of LC3 in U251 cells were observed after Capsaicin treatment. Meanwhile, the expressions of Beclin1, P62, and Puma-α increased. After using 3-MA, the expressions of Beclin1 and Procaspase-3 were reduced while those of P53 and Puma-α increased. The expression of LC3II was increased after Pifithrin-α treatment. Therefore, we believed that capsaicin could induce apoptosis in U251 cells, and the inhibition of autophagy could contribute to apoptosis. PMID:26351174

  8. High-Spin Structures in the N = 153 Nucleus 251Cf

    Science.gov (United States)

    Qiu, Y.; Hota, S. S.; Chowdhury, P.; Guess, C. J.; Jackson, E. G.; Lister, C. J.; Prasher, V. S.; Khoo, T. L.; Carpenter, M. P.; Janssens, R. V. F.; Greene, J.; Ahmad, I.; Seweryniak, D.; Zhu, S.; Albers, M.; Alcorta, M.; Bertone, P. F.; Chen, J.; Chiara, C. J.; Hoffman, C. R.; Kondev, F. G.; Lauritsen, T.; Tandel, S. K.

    2013-10-01

    In continuation of our exploration of band structures in neutron-rich Cf nuclei using inelastic and transfer reactions, we report new spectroscopic observations in the 251Cf nucleus. High-spin states of neutron-rich Cf nuclei were populated using a 208Pb beam from the ATLAS facility at Argonne, incident on a radioactive target mixture of 249 , 250 , 251Cf. Prompt γ rays were detected by the Gammasphere array. Both signatures of the ground state band of 251Cf were observed for the first time, with enhanced signal-to-noise achieved through appropriate gates on sum energy and fold parameters. Assignment of the band structure to 251Cf is via coincidence with Cf X-rays as well as the excitation of the 208Pb beam partner. Configurations are assigned to the observed band from experimental M1/E2 branching ratios from clean decays within the band. Further data analysis is in progress, and the new results will be discussed in the context of physics of the highest neutron orbitals accessible to spectroscopy in the A = 250 region. Work supported by U.S. Department of Energy.

  9. 37 CFR 251.56 - Order of the Librarian of Congress.

    Science.gov (United States)

    2010-07-01

    ... 37 Patents, Trademarks, and Copyrights 1 2010-07-01 2010-07-01 false Order of the Librarian of... PROCEDURE Procedures of Copyright Arbitration Royalty Panels § 251.56 Order of the Librarian of Congress. (a... the determination of a panel, the Librarian of Congress shall issue an order accepting the...

  10. 37 CFR 251.53 - Report to the Librarian of Congress.

    Science.gov (United States)

    2010-07-01

    ... 37 Patents, Trademarks, and Copyrights 1 2010-07-01 2010-07-01 false Report to the Librarian of... PROCEDURE Procedures of Copyright Arbitration Royalty Panels § 251.53 Report to the Librarian of Congress... Librarian of Congress a report incorporating its written determination. Such determination shall...

  11. 36 CFR 251.103 - Mediation of term grazing permit disputes.

    Science.gov (United States)

    2010-07-01

    ... permit, in whole or in part, as authorized by 36 CFR 222.4 (a)(2)(i), (ii), (iv), (v), and (a)(3) through... 36 Parks, Forests, and Public Property 2 2010-07-01 2010-07-01 false Mediation of term grazing... Lands § 251.103 Mediation of term grazing permit disputes. (a) Decisions subject to mediation. In...

  12. 37 CFR 251.44 - Filing and service of written cases and pleadings.

    Science.gov (United States)

    2010-07-01

    ... OF CONGRESS COPYRIGHT ARBITRATION ROYALTY PANEL RULES AND PROCEDURES COPYRIGHT ARBITRATION ROYALTY PANEL RULES OF PROCEDURE Procedures of Copyright Arbitration Royalty Panels § 251.44 Filing and service..., as necessary. In no case shall a party tender any written case or pleading by facsimile...

  13. 37 CFR 251.47 - Conduct of hearings: Witnesses and counsel.

    Science.gov (United States)

    2010-07-01

    ... OF CONGRESS COPYRIGHT ARBITRATION ROYALTY PANEL RULES AND PROCEDURES COPYRIGHT ARBITRATION ROYALTY PANEL RULES OF PROCEDURE Procedures of Copyright Arbitration Royalty Panels § 251.47 Conduct of hearings... may present its opening statement with the presentation of its direct case. (c) All witnesses shall...

  14. Radiosensitization and relative mechanisms of vanillin derivative BVAN08 on human glioma U-251 cells

    International Nuclear Information System (INIS)

    Objective: To provide more convincing evidences and experimental data for exploring vanillin derivative BVAN08, 6-bromine-5-hydroxy-4-methoxy-benzaldehyde, as a new anticancer drug, and to investigate the effect on the growth, radiosensitization of human glioma cell line U-251 and the relative mechanism. Methods: The effect of BVAN08 on cell proliferation of U-251 and radiosensitivity to 60Co γ-rays (irradiation dose rate 2.3 Gy/min) were analyzed with MTT and colony-forming ability assay. Change in cellular morphology was observed by using light microscope. Change in cell cycle and apoptosis was detected with flow cytometry. The autophagy was observed by using TEM (irradiation dose rate is transmission electron microscope). DNA-PKcs protein level was detected through Western blot analysis. Results: BVAN08 exhibited a dose- and time-dependent inhibition on the proliferation of U-251 cells during the concentration range of 10-100 mol/L (t=1.83-3.07, P50 at 48 h and 72 h after administration with BVAN08 were 55.3 and 52.7 mol/L, respectively. Obvious G2/M arrest was induced in U-251 cells after 4 h administration with BVAN08, and reached peck at 12 h. The G2/M population reached 63.3% in U-251 cells after 12 h administration of 60 μmol/L BVAN08 and kept increasing with the time, while both apoptosis and autophagic cell death were induced. The most effective radiosensitization time for BVAN08 treatment was 12 h before irradiation. The enhancement ratio of radiosensitivity was 3.14 for 20 μmol/L of BVAN08 12 h before 2 Gy irradiation. Conclusions: BVAN08 can induce apoptosis as radiosensitizing effect might be associated with the induction of G2/M arrest and inhibition of DNA-PKcs expression. BVAN08 seemed to be a promising radiosensitizing anticancer drug. (authors)

  15. Isoform of Vascular Endothelial Cell Growth Inhibitor (VEGI72-251) Increases Interleukin-2 Production by Activation of T Lymphocytes

    Institute of Scientific and Technical Information of China (English)

    Jing-Juan YAO; Min ZHANG; Xiao-Hui MIAO; Ping ZHAO; Shi-Ying ZHU; Hui DING; Zhong-Tian QI

    2006-01-01

    The objective of this study is to investigate the characteristics of the recombinant variant of human vascular endothelial cell growth inhibitor, VEGI72-251, and compare its biological activities with that of its prototype VEGI24-174. The recombinant plasmid containing the variant VEGI72-251 gene was constructed and expressed in Escherichia coli. The effects of the expressed VEGI72-251 on cell proliferations were checked in the human umbilical vein endothelial cell line and certain tumor cell lines (ECV304 and B 16). The inhibition of VEGI72-251 on angiogenesis was detected in the chorioallantoic membrane of chick embryos. In comparison with VEGI24-174, the recombinant human VEGI72-251 seems to have no effect on the proliferation of endothelial cells and the angiogenesis of the chorioallantoic membrane in vitro. An enzyme-linked immunosorbent assaybased method was used for the measurement of interleukin-2 (IL-2) production by peripheral blood monocytes (PBMCs) treated with VEGI72-251. PBMCs were pretreated with VEGI72-251 (1.25-12.50 μg/ml) for 24 h in vitro, and the IL-2 concentration in PBMC medium was increased from 354 pg/ml to 1256 pg/ml. It can be concluded that VEGI72-251 is able to increase the level of human IL-2 production by the activation of T lymphocytes. Differing from VEGI24-174 on anti-angiogenesis, VEGI72-251 may serve as an anti-cancer factor through its activation of T lymphocytes.

  16. Cytotoxic activity of allogeneic natural killer cells on U251 glioma cells in vitro.

    Science.gov (United States)

    Guo, Meng; Wu, Tingting; Wan, Lixin

    2016-07-01

    The present study aimed to observe the cytotoxic activity of allogeneic natural killer (NK) cells on U251 glioma cells and to investigate their mechanism of action to establish an effective treatment strategy for neuroglioma. Cell survival curves, colony formation assays and karyotype analysis were performed to investigate the characteristics of U251 glioma cells. The present study demonstrated that natural killer group 2, member D (NKG2D)‑major histocompatibility complex class I‑related chain A/B (MICA/B) interactions contributed to the cytotoxic effect of NK cells on K562 and U251 cells. In antibody‑blocking assays to inhibit NKG2D ligands, the cytotoxic activity was not completely attenuated, which suggested that other signaling pathways contribute to the cytotoxic activity of NK cells on tumor cells in addition to the NKG2D‑mediated activity. The present study identified that the expression levels of NKG2D ligands on the surface of target cells influenced the strength of the NK cell immune response. Furthermore, allogeneic NK cells were observed to kill glioma cells in vitro, and this anticancer activity is associated with the rate of NKG2D expression on the surface of glioma cells. PMID:27175912

  17. Complex based on Isthmin and RGD motif against glioma U251 cells%基于Isthmin和RGD基序的复合物对胶质瘤U251的治疗作用

    Institute of Scientific and Technical Information of China (English)

    陈宏颉; 曹磊; 王守森; 郑兆聪; 王如密; 汪君

    2011-01-01

    Objective To study the effect of complex based on Isthmin and RGD motif against glioma U251 cells.Methods The complex based on Isthmin and RGD motif was prepared,and the glioma U251 cell xenografts were established to observe the antitumor and antiangiogenesis effects of the complex in vivo and in vivo.Results The complex could induce U251 cell apoptosis and attack tumor endothelium cells,which inhibited the growth of glioma U251 cells and improved the lifespan of tumor bearing mice.Conclusion The complex based on Isthmin and RGD motif could dually target tumor and endothelium cells,which provided a promising strategy for glioma gene therapy.%目的 探讨基于Isthmin和BGD基序的复合物对胶质瘤U251的治疗效应.方法 构建Isthmin和RGD基序的复合物,并建立胶质瘤U251移植瘤模型,体内外观察其对U251细胞和血管内皮细胞的影响.结果 该复合物能有效诱导U251细胞的凋亡,抑制肿瘤血管的生成,从而抑制肿瘤的生长和延长荷瘤小鼠的平均生存率.结论 该复合物能同时靶向肿瘤和血管内皮细胞,为胶质瘤的基因治疗提供了较理想的策略.

  18. COST251 results on the ionospheric quasi-periodic variability caused by gravity and planetary waves - a review

    Czech Academy of Sciences Publication Activity Database

    Laštovička, Jan

    Didcot: CLRC Rutherford Appleton Laboratory, 1999 - (Vernon, A.), s. 142-154 [COST251 Workshop 'Impact of the Upper Atmosphere on Terrestrial and Earth-Space Communications' /4./. Madeira (PT), 22.03.1999-25.03.1999] R&D Projects: GA MŠk OC 251.10; GA ČR GA205/98/0058 Institutional research plan: CEZ:AV0Z3042911 Subject RIV: BL - Plasma and Gas Discharge Physics

  19. Study of apoptosis of glioma cells line U251 cells induced by sodium nitroprusside%硝普钠诱导胶质瘤细胞株U251细胞凋亡的研究

    Institute of Scientific and Technical Information of China (English)

    吴越; 徐国政; 杜浩; 吴长松; 黄河; 宋健

    2013-01-01

    Objective To investigate the effect of sodium nitroprusside(SNP) on glioma cell line U251 cells apoptosis and its mechanism.Methods U251 cells were cultured in the media containing SNP of the different concentrations.The rates of the inhibition of U251 cells growth induced by SNP were detected by MTT assay.Nitic oxide (NO) concentration in U251 cells was determined by Griess method.The rate of U251 cells apoptosis was detected by flow cytometry.The expression of Bcl-2 and caspase-3 were detected by western blot in U251 cells before and after the treatment with SNP.Results The significantly inhibitory effects of the different concentration SNP on U251 cells were observed (P<0.01) and they were SNP dose-dependent.The NO concentration rose with the increase in SNP concentration.There was significantly positive correlation between the inhibitory rate and NO concentration (P<0.01).The apoptotic rates of U251 cells in 0.5 mmol/L,1.0 mmol/L and 20 mmol/L SNP groups were 15.25%,20.56% and 40.73% respectively and they were significantly higher than that (5.04%) in the control group.The apoptotic effects of SNP on U251 cells were SNP dose-dependent (P<0.01).The expression level of Bcl-2 was significantly lower and the expression level of caspase-3 was significantly higher in all the experiment groups than those in the control group (P<0.05).The levels of Bcl-2 expression were negatively related and the levels of caspase-3 expression were positively related to SNP concentration (P<0.05).Conclusions SNP can inhibit the growth and induce apoptosis of U251 cells.The effect of SNP on of U251 cells growth may be related to NO released by SNP and the apoptotic effect of SNP on U251 cells may be related to the SNP-induced down-regulation of Bcl-2 and up-regulation of caspase-3 in of U251 cells.%目的 探讨一氧化氮(NO)供体药物硝普钠(SNP)对胶质瘤细胞株U251细胞凋亡诱导效应及其机制.方法 以0.2、0.5、1.5和2.0 mmol

  20. Effect of combination of STAT3 RNAi and 60Co γ-irradiation on U251 cell proliferation

    International Nuclear Information System (INIS)

    Objective: To construct signal transduction and activators of transcription 3 (STAT3) small interference RNA (siRNA) expression vector and to study its effect on STAT3 expression and U251 cell line proliferation. Methods: STAT3 specific 19 bp oligonucleotides were designed and synthesized. These oligonucleotides were annealed to form the double strand DNA fragments and these fragments were cloned into Psilence2.1-U6-H1 vector. The recombinant of STAT3-siRNA expressing construction was confirmed by Hind III and BamH I double digestion and sequencing. The STAT3-siRNA was transfected into U251 cell. The inhibitory effect of STAT3-siRNA construction was tested by Western blot. Cellular proliferation activities were measured by tetrazolium bromide (MTT) colorimetry. Cloning efficiency and MTF were used to confirm the radiation dose. Results: STAT3-siRNA expression vector was successfully constructed, and it could effectively down-regulate the protein levels of STAT3 in transfected U251 cell line; and the radiation dose was confirmed to 2 Gy. U251 cells transfected with STAT3-siRNA expression vector showed lower cellular proliferation compared with non-transfected U251 cells (P60Co γ-irradiation showed lower cellular proliferation compared with non-irradiated U251 cells (P60Co γ irradiation can enhance the inhibitory efficiency. (authors)

  1. 125I uptake in U251 glioma cell co-transfected with the human sodium/iodide symporter and the human thyroperoxidase

    International Nuclear Information System (INIS)

    Objective: To investigate the iodide uptake by U251 glioma cell lines which were transferred with both human sodium/iodide symporter (hNIS) and human thyroperoxidase (hTPO) genes. Methods: Recombinant adenosine virus AdTPO was constructed through cloning, recombination, packaging and amplifying. The viral titers were calculated after purification. The protein expression of AdTPO was tested by Western-Blotting and the recombinant plasmids PcDNA3. 1/hNIS were constructed. After hNIS gene was transfected into human glioma cell lines U251 through liposome, the cell lines with stable hNIS expression (hNIS-U251) selected by G418 antibiotics were defined as hNIS-U251 group. Then, hTPO was transduced into hNIS-U251 with adenosine virus (AdTPO-hNIS-U251 group). U251 cells with no plasmid were used as the control group (U251). Cultured cells from each group were studied for 125I uptake as well as 125I efflux rate. Student-Newman-Keuls in multiple range test was used. Results: AdTPO-hNIS-U251 with stable expression was successfully established by transfecting hNIS and hTPO genes into human glioma cell lines. The 125I uptake by AdTPO-hNIS-U251, hNIS-U251 and U251 cell lines was (74 647.53±3605.88), (55 769.96±4353.26) and (507.67±57.69) counts/min, respectively (F = 836. 17, P125I efflux rate was prolonged in AdTPO-hNIS-U251 group and its effective half time was 13 min. Conclusion: Enhanced 125I uptake by the human glioma cell lines can be achieved with combined transfection of hNIS and hTPO genes. (authors)

  2. [Protease and alpha-amylase synthesis by washed cells of Aspergillus oryzae 251-90].

    Science.gov (United States)

    Ustiuzhanina, S V; Iarovenko, V L; Voĭnarskiĭ, I N

    1985-01-01

    Regularities of protease and alpha-amylase production by washed cells of Aspergillus oryzae 251-90 were being studied. The results obtained enabled us to assume a constitutive character of the both enzymes synthesis by the given producer. Sources of carbon, nitrogen and sulphur take part in regulation of the protease production, whereas in the case of the alpha-amylase synthesis only carbon sources that are important. Elimination of phosphorus from the medium affects the synthesis of both enzymes. Celatin stimulates the production of the two enzymes, being a supplier of amino acids. PMID:3885211

  3. Fine mapping of chromosome 15q25.1 lung cancer susceptibility in African-Americans

    OpenAIRE

    Hansen, Helen M.; Xiao, Yuanyuan; Rice, Terri; Bracci, Paige M.; Wrensch, Margaret R.; Sison, Jennette D.; Chang, Jeffery S.; Smirnov, Ivan V.; Patoka, Joseph; Seldin, Michael F; Quesenberry, Charles P.; Kelsey, Karl T.; Wiencke, John K.

    2010-01-01

    Several genome-wide association studies identified the chr15q25.1 region, which includes three nicotinic cholinergic receptor genes (CHRNA5-B4) and the cell proliferation gene (PSMA4), for its association with lung cancer risk in Caucasians. A haplotype and its tagging single nucleotide polymorphisms (SNPs) encompassing six genes from IREB2 to CHRNB4 were most strongly associated with lung cancer risk (OR = 1.3; P < 10−20). In order to narrow the region of association and identify potential c...

  4. siRNA epidermal growth factor receptor silencing in U251 glioma cells

    Institute of Scientific and Technical Information of China (English)

    Chunsheng Kang; Zhiyong Zhang; Zhifan Jia; Qiang Huang; Guangxiu Wang; Mingzhe Qiu; Peiyu Pu

    2008-01-01

    BACKGROUND: Dicer, a large multidomain ribonuclease, is responsible for processing double-stranded RNAs (dsRNAs) to 20-bp-long small interfering RNAs (siRNAs), which act as effectors during RNA interference (RNAi). OBJECTIVE: To observe the efficacy of siRNA cocktails generated by recombinant human Dicer on the down-regulation of epidermal growth factor receptor (EGFR) expression in human glioma cells. DESIGN, TIME AND SETTING: The following in vitro experiment was performed at the Department of Neurosurgery, Tianjin Medical University General Hospital and Laboratory of Neuro-Oncology, Tianjin Neurological Institute. MATERIALS: Mini-RNA isolation kit, human placenta complimentary DNA (cDNA) was produced by Tiangen Biotech (Beijing, China), human glioblastoma U251-MG cells were produced by the Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences. METHODS: A PCR product from the human EGFR, which corresponded to the tyrosine kinase domain of the 3'-end fragment, was used as the T7-promotor for in vitro transcription, siRNA cocktails were generated by in vitro dicing of double stranded RNA. A total of 500, 250 and 125 μg siRNA cocktails were transiently transfected into U251 glioma cells through the use of the GeneSilencer. MAIN OUTCOME MEASURE: Expression of EGFR was detected by real-time PCR. RESULTS: The total PCR product of the human EGFR, corresponding to the tyrosine kinase domain, is approximately 680 bp in length. The PCR transcriptants included GCC leader sequences and a T7 promoter sequence, with a fragment of EGFR cDNA at the center. The T7 promoter was prepared for in vitro transcription of dsRNA. After dicing for 24 hours, the 21-nt siRNA cocktails were verified by 4% agarose gel. The difference between threshold cycle of a sample assay and threshold cycle of the corresponding endogenous reference (△ Ct) among parental U251 cells and cells transfected with different doses of siRNA cocktails were determined to be 3.06, 7.35, and 10

  5. The study on the mechanism of enhanced radio-sensitivity of glioma cell U251 by thalidomide

    International Nuclear Information System (INIS)

    In order to investigate the inhibition of DNA synthesis and the mechanism of thalidomide enhanced radio-sensitivity on glioma U251 cells, survival rate of the cells was assessed by MTT assay; and 3H-TdR incorporation assay was used to investigate the inhibition of DNA synthesis by thalidomide alone and combined with X-rays; VEGF mRNA expressions were evaluated using RT-PCR assay. Protein expression (VEGF) was detected by Western blot analysis. Thalidomide inhibited DNA synthesis depending on the concentration of thalidomide and radiation dose. Thalidomide markedly down-regulated VEGF mRNA combined with X-rays in which a synergistic action appeared. Low dose X-rays can induce VEGF protein expression in 24 h. Thalidomide was a low toxicity to U251 cells. Thalidomide inhibited DNA synthesis of U251 cells. (authors)

  6. Fine mapping of chromosome 15q25.1 lung cancer susceptibility in African-Americans.

    Science.gov (United States)

    Hansen, Helen M; Xiao, Yuanyuan; Rice, Terri; Bracci, Paige M; Wrensch, Margaret R; Sison, Jennette D; Chang, Jeffery S; Smirnov, Ivan V; Patoka, Joseph; Seldin, Michael F; Quesenberry, Charles P; Kelsey, Karl T; Wiencke, John K

    2010-09-15

    Several genome-wide association studies identified the chr15q25.1 region, which includes three nicotinic cholinergic receptor genes (CHRNA5-B4) and the cell proliferation gene (PSMA4), for its association with lung cancer risk in Caucasians. A haplotype and its tagging single nucleotide polymorphisms (SNPs) encompassing six genes from IREB2 to CHRNB4 were most strongly associated with lung cancer risk (OR = 1.3; P < 10(-20)). In order to narrow the region of association and identify potential causal variations, we performed a fine-mapping study using 77 SNPs in a 194 kb segment of the 15q25.1 region in a sample of 448 African-American lung cancer cases and 611 controls. Four regions, two SNPs and two distinct haplotypes from sliding window analyses, were associated with lung cancer. CHRNA5 rs17486278 G had OR = 1.28, 95% CI 1.07-1.54 and P = 0.008, whereas CHRNB4 rs7178270 G had OR = 0.78, 95% CI 0.66-0.94 and P = 0.008 for lung cancer risk. Lung cancer associations remained significant after pack-year adjustment. Rs7178270 decreased lung cancer risk in women but not in men; gender interaction P = 0.009. For two SNPs (rs7168796 A/G and rs7164594 A/G) upstream of PSMA4, lung cancer risks for people with haplotypes GG and AA were reduced compared with those with AG (OR = 0.56, 95% CI 0.38-0.82; P = 0.003 and OR = 0.73, 95% CI 0.59-0.90, P = 0.004, respectively). A four-SNP haplotype spanning CHRNA5 (rs11637635 C, rs17408276 T, rs16969968 G) and CHRNA3 (rs578776 G) was associated with increased lung cancer risk (P = 0.002). The identified regions contain SNPs predicted to affect gene regulation. There are multiple lung cancer risk loci in the 15q25.1 region in African-Americans. PMID:20587604

  7. 46 CFR 251.21 - Applications under sections 803, 804, 805 (a) and (d), and 605 (b), Merchant Marine Act, 1936.

    Science.gov (United States)

    2010-10-01

    ...), and 605 (b), Merchant Marine Act, 1936. 251.21 Section 251.21 Shipping MARITIME ADMINISTRATION... (b), Merchant Marine Act, 1936. Form VI-B of instructions is the required form for the preparation of... chapter (General Order 86)) and (d), and 605(b), Merchant Marine Act, 1936 (49 Stat. 2012, 2013, 2003;...

  8. Transient axonal glycoprotein-1 induces apoptosis-related gene expression without triggering apoptosis in U251 glioma cells

    Institute of Scientific and Technical Information of China (English)

    Haigang Chang; Xiaodan Jiang; Shanshan Song; Zhongcan Chen; Yaxiao Wang; Lujun Yang; Mouxuan Du; Yiquan Ke; Ruxiang Xu; Baozhe Jin

    2014-01-01

    Previous studies show that transient axonal glycoprotein-1, a ligand of amyloid precursor pro-tein, increases the secretion of amyloid precursor protein intracellular domain and is involved in apoptosis in Alzheimer’s disease. In this study, we examined the effects of transient axonal glyco-protein-1 on U251 glioma cells. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay showed that transient axonal glycoprotein-1 did not inhibit the proliferation of U251 cells, but promoted cell viability. The terminal deoxynucleotidyl transferase dUTP nick end labeling assay showed that transient axonal glycoprotein-1 did not induce U251 cell apoptosis. Real-time PCR revealed that transient axonal glycoprotein-1 substantially upregulated levels of amyloid precursor protein intracellular C-terminal domain, and p53 and epidermal growth factor recep-tor mRNA expression. Thus, transient axonal glycoprotein-1 increased apoptosis-related gene expression in U251 cells without inducing apoptosis. Instead, transient axonal glycoprotein-1 promoted the proliferation of these glioma cells.

  9. 48 CFR 252.251-7001 - Use of Interagency Fleet Management System (IFMS) vehicles and related services.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 3 2010-10-01 2010-10-01 false Use of Interagency Fleet... Fleet Management System (IFMS) vehicles and related services. As prescribed in 251.205, use the following clause: Use of Interagency Fleet Management System (IFMS) Vehicles and Related Services (DEC...

  10. Risk modification of colorectal cancer susceptibility by interleukin-8-251T>A polymorphism in Malaysians

    Institute of Scientific and Technical Information of China (English)

    Mohd Aminudin Mustapha; Siti Nurfatimah Mohd Shahpudin; Ahmad Aizat Abdul Aziz; Ravindran Ankathil

    2012-01-01

    AIM:To investigate the allele and genotype frequencies and associated risk of interleukin (IL)-8-251T>A polymorphism on colorectal cancer (CRC) susceptibility risk.METHODS:Peripheral blood samples of 255 normal controls and 255 clinically and histopathologically confirmed CRC patients were genotyped for IL-8-251T>A polymorphism employing allele-specific polymerase chain reaction.The relative association of variant allele and genotypes with CRC susceptibility risk was determined by calculating the odds ratios (ORs).Corresponding x2 tests on the CRC patients and controls were carried out and 95% confidence intervals (CIs) were determined using Fisher's exact test.The allele frequencies and its risk association were calculated using FAMHAP,haplotype association analysis software.RESULTS:On comparing the frequencies of genotypes of patients and controls,the homozygous variant AA was significantly higher in CRC patients (P =0.002)compared to controls.Investigation on the association of the polymorphic genotypes with CRC susceptibility risk,showed that the homozygous variant IL-8-251AA had a significantly increased risk with OR 3.600 (95%CI:1.550-8.481,P =0.001).In the case of allele frequencies,variant allele A of IL-8-251 showed a significantly increased risk of CRC predisposition with OR 1.32(95% CI:1.03-1.69,P =0.003).CONCLUSION:Variant allele and genotype of IL-8 (-251T>A) was significantly associated with CRC susceptibility risk and could be considered as a high-risk variant for CRC predisposition.

  11. Adenovirus-mediated transfer of p53 augments hyperthermia-induced apoptosis in U251 glioma cells

    International Nuclear Information System (INIS)

    Purpose: Hyperthermia kills glioma cells by inducing apoptosis and is thereby an effective therapeutic modality for the treatment of malignant gliomas. However, cells harboring mutated p53 are refractory to hyperthermia-induced apoptosis. In this study, we assessed whether or not adenovirus (Adv)-mediated transduction of p53 overrides this resistant mechanism. Methods and Materials: We transduced the p53 wild-type tumor suppressor gene into U251 glioma cells harboring mutated p53 using Adv vectors in combination with hyperthermia (43, 44.5 deg. C), and evaluated the degree of cell death and apoptosis. Results: The percentage of cells that had died, as measured by trypan blue staining, among U251 cells infected with the Adv for p53 (Adv-p53) and treated with hyperthermia, was significantly higher than the percentage of cells that had died among U251 cells infected with Adv-p53 and not treated with hyperthermia, or those infected with the control Adv for dE (Adv-dE) and treated with hyperthermia. The degree of apoptosis, measured at 24 h after treatment, in hyperthermia-treated U251 cells infected with Adv-p53 (43 deg. C, 73%; 44.5 deg. C, 92%) was much higher than that infected with Adv-p53 (41%), or that infected with control Adv-dE and treated with hyperthermia (43 deg. C, 1.3%; 44.5 deg. C, 19%). Treatment with combined hyperthermia and Adv-p53 infection induced cleavage of caspase-3 in U251 cells. Conclusion: These results indicate that Adv-mediated transduction of p53 would render glioma cells highly sensitive to hyperthermia

  12. Nematode assemblages from the Kandalaksha Depression (White Sea, 251-288 m water depth)

    Science.gov (United States)

    Miljutin, Dmitry M.; Miljutina, Maria A.; Tchesunov, Alexei V.; Mokievsky, Vadim O.

    2014-03-01

    The shallow-water nematodes of the White Sea are relatively well studied; however, information on the nematode fauna inhabiting the deepest part of this sea is very scarce. The composition of the nematode assemblages (at species and genus level) was studied in samples collected during four sampling occasions in the deepest part of the Kandalaksha Depression (the White Sea) in July 1998, October 1998, May 1999, and November 1999. Samples were collected from a depth of 251-288 m with the aid of a multicorer. In total, 59 nematode morphotypes belonging to 37 genera and 18 families were distinguished. The genera Sabatieria and Filipjeva dominated at all stations, followed by Aponema, Desmoscolex, and Quadricoma. The composition of the dominant genera can be considered typical for this depth range in temperate and Arctic waters, although Filipjeva and Aponema were among the dominant genera for the first time. The most abundant species were Sabatieria ornata, Aponema bathyalis, and Filipjeva filipjevi. In general, diversity of the nematode assemblages was lower than in the temperate and Arctic continental shelf and slope with reduced evenness and species richness. The evenness of nematode assemblages and other diversity indices decreased with increasing sediment depth. Based on the valid species and genera recorded, the nematode fauna of the Kandalaksha Depression showed a higher resemblance to that found in the shallow waters of Kandalaksha Bay.

  13. More Evidence for the Intermediate Broad Line Region of the Mapped AGN PG 0052+251

    CERN Document Server

    Zhang, XueGuang

    2013-01-01

    In the manuscript, the properties of the proposed intermediate BLR are checked for the mapped AGN PG 0052+251. With the considerations of the apparent effects of the broad He {\\sc ii} line on the observed broad H$\\beta$ profile, the line parameters (especially the line width and the line flux) of the observed broad H$\\alpha$ and the broad H$\\beta$ are carefully determined. Based on the measured line parameters, the model with two broad components applied for each observed broad balmer line is preferred, and then confirmed by the calculated much different time lags for the inner/intermediate broad components and the corresponding virial BH masses ratio determined by the properties of the inner and the intermediate broad components. Then, the correlation between the broad line width and the broad line flux is checked for the two broad components: one clearly strong negative correlation for the inner broad component, but one positive correlation for the intermediate broad component. The different correlations fo...

  14. Evidence for Intermediate BLR of Reverberation-Mapped AGN PG 0052+251

    CERN Document Server

    Zhang, Xue-Guang

    2011-01-01

    In this manuscript, we study properties of BLR of well-known reverberation-mapped AGN, in order to find reliable evidence for intermediate BLR. We firstly check properties of mapped AGN collected from literature in plane of $\\sHb^2/\\sHa^2$ vs $\\RHa/\\RHb$. Commonly, virial BH masses based on observed broad H$\\alpha$ and H$\\beta$ should be coincident. However, among the mapped objects, PG0052 and NGC4253 are two apparent outliers in the plane of $\\sHb^2/\\sHa^2$ vs $\\RHa/\\RHb$, which indicate BLRs of PG0052 and NGC4253 have some special characters. Then based on the 55 public spectra of PG0052, BLR of PG0052 is been carefully studied in detail. We find that line width ratio of total observed broad H$\\alpha$ to total observed broad H$\\beta$ is $\\sim$0.7, which is much smaller than theoretical/observational value of $\\sim$0.9. Furthermore, flux ratio of total broad H$\\alpha$ to total broad H$\\beta$ is about 6.8 (Balmer Decrement), which is not one reasonable value for BLUE quasar PG 0052+251. Moreover, properties ...

  15. Exploring High AR 2 μm TSV (25:1)

    Science.gov (United States)

    Kostermans, Maarten; Baier, Ulrich; Boullart, Werner; Brouri, Mohand; Vertommen, Johan; Pageau, Arnaud

    2011-10-01

    3D integration allows for reduction of the system size, both in area and volume. It improves performance since 3D interconnects are shorter than traditional interconnects in a 2D configuration, enabling a higher operation speed and smaller power consumption. In order to ensure a reliable pattern transfer, a basic requirement for the etch mechanism is to provide anisotropy. Traditionally, through-silicon vias (TSVs) are achieved by means of the Bosch process, where anisotropic etch occurs via cycles of deposition and etch steps. This work investigates the etch process for 2 μm diameter vias with an aspect ratio up to 25:1, by means of an industrial ICP etch chamber with pulsed low frequency bias and ramping of different parameters like gasflows, bias voltage and separate etch and deposition step times. In order to reduce bowing while maintaining acceptable top/bottom CDs and reduce sidewall roughness, mechanisms of sidewall passivation have been studied by changing bias power duty cycle, modifying gas flow ramps, and O2 addition. Finally, scaling rules have been established that allow to predict etch time for various TSV diameters and A/R.

  16. Adjuvant-dependent innate and adaptive immune signatures of risk of SIVmac251 acquisition.

    Science.gov (United States)

    Vaccari, Monica; Gordon, Shari N; Fourati, Slim; Schifanella, Luca; Liyanage, Namal P M; Cameron, Mark; Keele, Brandon F; Shen, Xiaoying; Tomaras, Georgia D; Billings, Erik; Rao, Mangala; Chung, Amy W; Dowell, Karen G; Bailey-Kellogg, Chris; Brown, Eric P; Ackerman, Margaret E; Vargas-Inchaustegui, Diego A; Whitney, Stephen; Doster, Melvin N; Binello, Nicolo; Pegu, Poonam; Montefiori, David C; Foulds, Kathryn; Quinn, David S; Donaldson, Mitzi; Liang, Frank; Loré, Karin; Roederer, Mario; Koup, Richard A; McDermott, Adrian; Ma, Zhong-Min; Miller, Christopher J; Phan, Tran B; Forthal, Donald N; Blackburn, Matthew; Caccuri, Francesca; Bissa, Massimiliano; Ferrari, Guido; Kalyanaraman, Vaniambadi; Ferrari, Maria G; Thompson, DeVon; Robert-Guroff, Marjorie; Ratto-Kim, Silvia; Kim, Jerome H; Michael, Nelson L; Phogat, Sanjay; Barnett, Susan W; Tartaglia, Jim; Venzon, David; Stablein, Donald M; Alter, Galit; Sekaly, Rafick-Pierre; Franchini, Genoveffa

    2016-07-01

    A recombinant vaccine containing Aventis Pasteur's canarypox vector (ALVAC)-HIV and gp120 alum decreased the risk of HIV acquisition in the RV144 vaccine trial. The substitution of alum with the more immunogenic MF59 adjuvant is under consideration for the next efficacy human trial. We found here that an ALVAC-simian immunodeficiency virus (SIV) and gp120 alum (ALVAC-SIV + gp120) equivalent vaccine, but not an ALVAC-SIV + gp120 MF59 vaccine, was efficacious in delaying the onset of SIVmac251 in rhesus macaques, despite the higher immunogenicity of the latter adjuvant. Vaccine efficacy was associated with alum-induced, but not with MF59-induced, envelope (Env)-dependent mucosal innate lymphoid cells (ILCs) that produce interleukin (IL)-17, as well as with mucosal IgG to the gp120 variable region 2 (V2) and the expression of 12 genes, ten of which are part of the RAS pathway. The association between RAS activation and vaccine efficacy was also observed in an independent efficacious SIV-vaccine approach. Whether RAS activation, mucosal ILCs and antibodies to V2 are also important hallmarks of HIV-vaccine efficacy in humans will require further studies. PMID:27239761

  17. Efficient transmission and persistence of low-frequency SIVmac251 variants in CD8-depleted rhesus macaques with different neuropathology

    OpenAIRE

    Strickland, Samantha L.; Gray, Rebecca R; Lamers, Susanna L.; Burdo, Tricia H.; Huenink, Ellen; Nolan, David J.; Nowlin, Brian; Alvarez, Xavier; Midkiff, Cecily C.; Goodenow, Maureen M.; Williams, Kenneth; Salemi, Marco

    2012-01-01

    Infection of CD8-depleted rhesus macaques with the genetically heterogeneous simian immunodeficiency virus (SIV)mac251 viral swarm provides a rapid-disease model for simian acquired immune deficiency syndrome and SIV-encephalitis (SIVE). The objective was to evaluate how the diversity of the swarm influences the initial seeding of the infection that may potentially affect disease progression. Plasma, lymphoid and non-lymphoid (brain and lung) tissues were collected from two infected macaques ...

  18. Reciprocal regulation of Abl kinase by Crk Y251 and Abi1 controls invasive phenotypes in glioblastoma

    Science.gov (United States)

    Kumar, Sushil; Lu, Bin; Dixit, Updesh; Hossain, Sajjad; Liu, Yongzhang; Li, Jing; Hornbeck, Peter; Zheng, Weiming; Sowalsky, Adam G.; Kotula, Leszek; Birge, Raymond B.

    2015-01-01

    Crk is the prototypical member of a class of Src homology 2 (SH2) and Src homology 3 (SH3) domain-containing adaptor proteins that positively regulate cell motility via the activation of Rac1 and, in certain tumor types such as GBM, can promote cell invasion and metastasis by mechanisms that are not well understood. Here we demonstrate that Crk, via its phosphorylation at Tyr251, promotes invasive behavior of tumor cells, is a prominent feature in GBM, and correlating with aggressive glioma grade IV staging and overall poor survival outcomes. At the molecular level, Tyr251 phosphorylation of Crk is negatively regulated by Abi1, which competes for Crk binding to Abl and attenuates Abl transactivation. Together, these results show that Crk and Abi1 have reciprocal biological effects and act as a molecular rheostat to control Abl activation and cell invasion. Finally, these data suggest that Crk Tyr251 phosphorylation regulate invasive cell phenotypes and may serve as a biomarker for aggressive GBM. PMID:26473374

  19. Human Cytomegalovirus-Encoded miR-US25-1 Aggravates the Oxidised Low Density Lipoprotein-Induced Apoptosis of Endothelial Cells

    Directory of Open Access Journals (Sweden)

    Jianmin Fan

    2014-01-01

    Full Text Available Human cytomegalovirus (HCMV infection is linked to the development and severity of the cardiovascular disease atherosclerosis; however, there is little known about the promotion of atherosclerosis. miR-US25-1 is one of HCMV-encoded miRNAs and targets cellular genes that are essential for virus growth to control the life cycle of the virus and host cells. The prominent regulation on cell cycle genes of the miR-US25-1 attracts us to explore its role in the atherosclerosis promotion. It was indicated that miR-US25-1 level was upregulated in subjects or in endothelial cells with HCMV infection; and the miR-US25-1 downregulated the expression of BRCC 3 by targeting the 5′ UTR of BRCC 3. And a miR-US25-1 mimics transfection could reduce the EAhy926 cell viability but did not induce apoptosis in EAhy926 cells. And what is more, miR-US25-1 mimicis transfection deteriorated the ox-LDL-induced apoptosis and aggravated the upregulation of apoptosis-associated molecules by oxidised low density lipoprotein (ox-LDL in EAhy926 cells. And we have also confirmed the deregulation of BRCC 3 expression by miR-US25-1 by targeting the 5′ UTR of it. Given the vital role of BRCC 3 in DNA damage repairing, we speculated that the targeting inhibition of BRCC 3 by miR-US25-1 may contribute to the aggravation of ox-LDL-promoted apoptosis of endothelial EAhy926 cells.

  20. The spiral-compact galaxy pair AM 2208-251: Computer simulations versus observations

    Science.gov (United States)

    Klaric, Mario; Byrd, Gene G.

    1990-01-01

    The system AM2208-251 is a roughly edge-on spiral extending east-west with a smaller round compact E system about 60 arcsec east of the spiral nucleus along the major axis of the spiral. Bertola, Huchtmeier, and Zeilinger (1990) have presented optical spectroscopic as well as single dish 21 cm observations of this system. Their spectroscopic data show, via emission lines lambda lambda 3727-29A, a rising rotation curve near the nucleus. These spectroscopic observations may indicate a tidal interaction in the system. In order to learn more about such pairs, the authors simulated the interaction using the computer model developed by Miller (1976 a,b, 1978) and modified by the authors (Byrd 1986, 1987, 1988). To do the simulation they need an idea of the mutual orbits of the two galaxies. Their computer model is a two-dimensional polar N-body program. It consists of a self-gravitating disk of particles, within an inert axially symmetric stabilizing halo potential. The particles are distributed in a 24(radial) by 36(azimuthal) polar grid. Self consistent calculations can be done only within the grid area. The disk is modeled with a finite Mestel disk, where all the particles initially move in circular orbits with constant tangential velocities (Mestel 1963), resulting in a flat rotation curve. The gas particles in the spiral's disk, which make up 30 percent of its mass, collide in the following manner. The number of particles in each bin of the polar grid is counted every time step. If it is greater than a given critical density, all the particles in the bin collide, obtaining in the result the same velocities, equal to the average for the bin. This process produces clumps of gas particles-the star formation sites. The authors suppress the collision in the inner part of the disk (within the circle r = 6) to represent the hole seen in the gas in the nuclear bulge of spirals. They thus avoid spurious effects due to collisions in that region. They also varied the size of

  1. The spiral-compact galaxy pair AM 2208-251: Computer simulations versus observations

    Science.gov (United States)

    Klaric, Mario; Byrd, Gene G.

    1990-11-01

    The system AM2208-251 is a roughly edge-on spiral extending east-west with a smaller round compact E system about 60 arcsec east of the spiral nucleus along the major axis of the spiral. Bertola, Huchtmeier, and Zeilinger (1990) have presented optical spectroscopic as well as single dish 21 cm observations of this system. Their spectroscopic data show, via emission lines lambda lambda 3727-29A, a rising rotation curve near the nucleus. These spectroscopic observations may indicate a tidal interaction in the system. In order to learn more about such pairs, the authors simulated the interaction using the computer model developed by Miller (1976 a,b, 1978) and modified by the authors (Byrd 1986, 1987, 1988). To do the simulation they need an idea of the mutual orbits of the two galaxies. Their computer model is a two-dimensional polar N-body program. It consists of a self-gravitating disk of particles, within an inert axially symmetric stabilizing halo potential. The particles are distributed in a 24(radial) by 36(azimuthal) polar grid. Self consistent calculations can be done only within the grid area. The disk is modeled with a finite Mestel disk, where all the particles initially move in circular orbits with constant tangential velocities (Mestel 1963), resulting in a flat rotation curve. The gas particles in the spiral's disk, which make up 30 percent of its mass, collide in the following manner. The number of particles in each bin of the polar grid is counted every time step. If it is greater than a given critical density, all the particles in the bin collide, obtaining in the result the same velocities, equal to the average for the bin. This process produces clumps of gas particles-the star formation sites. The authors suppress the collision in the inner part of the disk (within the circle r = 6) to represent the hole seen in the gas in the nuclear bulge of spirals. They thus avoid spurious effects due to collisions in that region. They also varied the size of

  2. Mechanism of thalidomide to enhance cytotoxicity of temozolomide in U251-MG glioma cells in vitro

    Institute of Scientific and Technical Information of China (English)

    GAO Song; YANG Xue-jun; ZHANG Wen-gao; JI Yan-wei; PAN Qiang

    2009-01-01

    Background Glioma is the most common primary brain tumor with poor prognosis. Temozolomide has been used with thalidomide to treat gliomas. We investigated the synergistic mechanism of these two drugs in vitro.Methods Human malignant glioma cells U251-MG were cultured and assigned to four groups with different treatments for 3 days: temozolomide group (100 pmol/L), thalidomide group (100 pg/L), temozolomide (100 IJmol/L) plus thalidomide group (100 pg/L) and control group. MTT assay was applied to evaluate the cell viability. Cell cycle was analyzed by flow cytometry. The ultra-structural features of autophagosomes were observed with electron microscope. Acridine orange and monodansylcadavedne were adopted to label autophagosomes and flow cytometry was applied for quantification of autophagosomes. The expression of autophagy-associated protein was detected by Western blotting.Results Proliferation of tumor cell was obviously suppressed by temozolomide with thalidomide treatment than by either drug used alone (P=-0.000 for each day). The combination treatment induced cell cycle arrest at G0/G1 phase.Typical autophagic ultra-structural character was found after the combined treatment. Thalidomide promoted the autophagy induced by temozolomide. The autophagy-associated proteins - microtubule associated protein 1 light chain 3 (MAPILC3) and Beclinl were more significantly up-regulated by the combined treatment than temozolomide used alone (MAP1LC3, P=-0.000; Beclinl, P=-0.004). The expression level of phosphatase and tensin homolog deleted on chromosome ten (PTEN), which promoted autophagy by suppressing PI3K/Akt/mTOR signaling pathway, was elevated by thalidomide (thalidomide group: P=-0.000; combined group: P=0.002).Conclusions Thalidomide enhances the cytotoxicity of temozolomide by promoting the autophagy induced by temozolomide. Contributing to the up-regulation of PTEN by thalidomide, the expression of autophagy associated protein-MAP1LC3 and Beclinl was enhanced

  3. A clinical epidemiological study of 251 cases of amyotrophic lateral sclerosis in the south of Brazil Estudo clínico epidemiológico de 251 casos de esclerose lateral amiotrófica no sul do Brasil

    OpenAIRE

    Lineu Cesar Werneck; Ruth Bezerra; Octavio da Silveira Neto; Rosana Herminia Scola

    2007-01-01

    OBJECTIVE: To study the clinical forms of amyotrophic lateral sclerosis (ALS) and the possible presence of risk factors in order to verify if there is any difference between cases in Paraná, Brazil. METHOD: We studied 251 cases, all of which fulfilled the diagnosis criteria proposed in El Escorial (WFN). Between 1977 and 2004, 157 male and 94 female patients were examined. RESULTS: 220 cases were classified as ALS-Spinal Onset (ALS-SO), 24 as ALS-Bulbar Onset (ALS-BO) and 7 as Familial ALS. T...

  4. Adenovirus-mediated delivery of bFGF small interfering RNA increases levels of connexin 43 in the glioma cell line, U251

    Directory of Open Access Journals (Sweden)

    Liu Hongsheng

    2010-01-01

    Full Text Available Abstract Background bFGF is an important growth factor for glioma cell proliferation and invasion, while connexin 43 is implicated in the suppression of glioma growth. Correspondingly, gliomas have been shown to have reduced, or compromised, connexin 43 expression. Methods In this study, a bFGF-targeted siRNA was delivered to the glioma cell line, U251, using adenovirus (Ad-bFGF-siRNA and the expression of connexin 43 and its phosphorylation state were evaluated. U251 cells were infected with Ad-bFGF-siRNA (100, 50, or 25 MOI, and infection with adenovirus expressing green fluorescent protein (Ad-GFP at 100 MOI served as a control. Western blotting and immunofluorescence were used to detect the expression levels, phosphorylation, and localization of connexin 43 in U251 cells infected, and not infected, with Ad-bFGF-siRNA. Results Significantly higher levels of connexin 43 were detected in U251 cells infected with Ad-bFGF-siRNA at 100 and 50 MOI than in cells infected with Ad-GFP, and the same amount of connexin 43 was detected in Ad-GFP-infected and uninfected U251 cells. Connexin 43 phosphorylation did not differ between Ad-bFGF-siRNA-infected and uninfected U251 cells. However, the ratio of phosphorylated to unphosphorylated connexin 43 in Ad-bFGF-siRNA cells was lower, and connexin 43 was predominantly localized to the cytoplasm. Using a scrape loading dye transfer assay, more Lucifer Yellow was transferred to neighboring cells in the Ad-bFGF-siRNA treated group than in the control group. Conclusion To our knowledge, this is the first description of a role for connexin 43 in the inhibition of U251 growth using Ad-bFGF-siRNA.

  5. On-line coupling of the MAT 251 with a Carlo Erba elemental analyzer for carbon isotope ratio measurements

    International Nuclear Information System (INIS)

    For carbon isotope investigations with a moderate precision demand of about 0.2 per mil in the isotope ratio fast and reliable results are attained by on line combination of the ANA 1500 Elemental Analyzer and the MAT 251 Isotope Mass Spectrometer. The crucial point hereof is the gas splitting device. By proper design and adjustment of the analytical parameters, good sample efficiency and a sharp CO2 bulk within the He stream is reached. The main characteristics of this combined equipment are described and some isotopic results of organic and anorganic carbon in lake sediment-samples are given as well as deltasup13C-analyses of spiritous liquors. (Author)

  6. Preparation of iodine-123 labeled AM251: a potential SPECT radioligand for the brain cannabinoid CB1 receptor

    International Nuclear Information System (INIS)

    We report the synthesis and labeling with iodine-123 of N-(piperidin-1-yl)-5-(4-iodophenyl)-1-(2, 4-dichlorophenyl)-4-methyl-1H-pyrazole-3-carboxamide (AM251). This compound is an analog of the recently described cannabinoid receptor antagonist, SR141716A, in which a 4-chlorophenyl group is replaced by 4-iodophenyl. Labeling in good yield (62%) and radiochemical purity (> 95%), and high specific activity (> 2500 Ci/mmol) was achieved by an iododestannylation reaction using the tributyltin precursor, no carrier added I-123 iodide, and chloramine-T. (author)

  7. Preparation of iodine-123 labeled AM251: a potential SPECT radioligand for the brain cannabinoid CB1 receptor

    Energy Technology Data Exchange (ETDEWEB)

    Lan, Ruoxi; Makriyannis, Alexandros [Connecticut Univ., Molecular and Cell Biology Dept., Storrs, CT (United States); Gatley, S.J. [Brookhaven National Lab., Medical Dept., Upton, NY (United States)

    1996-10-01

    We report the synthesis and labeling with iodine-123 of N-(piperidin-1-yl)-5-(4-iodophenyl)-1-(2, 4-dichlorophenyl)-4-methyl-1H-pyrazole-3-carboxamide (AM251). This compound is an analog of the recently described cannabinoid receptor antagonist, SR141716A, in which a 4-chlorophenyl group is replaced by 4-iodophenyl. Labeling in good yield (62%) and radiochemical purity (> 95%), and high specific activity (> 2500 Ci/mmol) was achieved by an iododestannylation reaction using the tributyltin precursor, no carrier added I-123 iodide, and chloramine-T. (author).

  8. The effect of combination of ErbB2 RNAi and 60Co γ-irradiation on U251 cell apoptosis

    International Nuclear Information System (INIS)

    To construct erythroblastic leukemia viral oncogene homolog 2 (ErbB2) small interference RNA (siRNA) expression vector and to study its effect on U251 cell line proliferation and apoptosis combining with 60Co γ-irradiation. ErbB2 specific 19bp oligonucleotides were designed and synthesized. These oligonucleotides were annealed to form the double strand DNA fragments, which was cloned into pSilence2.1-U6-H1 vector. The recombinant pSilence2.1-ErbB2 expression construct was confirmed by Hind III and BamH I double digestion and sequencing. The pSilence 2.1-ErbB2 was transfected into U251 cell. Cellular proliferation activities were assayed by tetrazolium bromide (MTT) colorimetry. The apoptosis of transfected U251 cell was examined with Hoechst 33258 staining and Annexin-V kit. Psilence 2.1-ErbB2 expression vector was successfully constructed and it can effectively inhibit pro- liferation(p60Co γ-irradiation, the effect of inhibiting proliferation was more significant compared with non-irradiated U251 cells(p60Co γ-irradiation can enhance the inhibitory efficiency in U251 cell line. (authors)

  9. Impact of carrier recombination on fill factor for large area heterojunction crystalline silicon solar cell with 25.1% efficiency

    Science.gov (United States)

    Adachi, Daisuke; Hernández, José Luis; Yamamoto, Kenji

    2015-12-01

    We have achieved a certified 25.1% conversion efficiency in a large area (151.9 cm2) heterojunction (HJ) crystalline Si (c-Si) solar cell with amorphous Si (a-Si) passivation layer. This efficiency is a world record in a both-side-contacted c-Si solar cell. Our high efficiency HJ c-Si solar cells are investigated from the standpoint of the effective minority carrier lifetime (τe), and the impact of τe on fill factor (FF) is discussed. The τe measurements of our high efficiency HJ c-Si solar cells reveal that τe at an injection level corresponding to an operation point of maximum power is dominated by the carrier recombination at the a-Si/c-Si interface. By optimization of the process conditions, the carrier recombination at the a-Si/c-Si interface is reduced, which leads to an improvement of the FF by an absolute value of 2.7%, and a conversion efficiency of 25.1% has been achieved. These results indicate that the reduction of carrier recombination centers at the a-Si/c-Si interface should be one of the most crucial issues for further improvement of FF even in the HJ c-Si solar cells with efficiency over 25%.

  10. Energy levels of 251Cf populated in the α decay of 100255Fm and EC decay of 99251Es

    International Nuclear Information System (INIS)

    Gamma-ray singles spectra of extremely pure (chemically and isotopically) samples of 255Fm, with strengths of ∼1 mCi, have been measured with a high-resolution 2-cm2x10-mm germanium LEPS detector and with a 25% Ge spectrometer. Gamma rays with intensities as low as 1.0x10-6% per 255Fm α decay have been identified. The electron spectrum of a mass-separated 251Es source was measured with a cooled Si(Li) electron spectrometer. The spectrum provided the conversion coefficients of low-energy transitions in 251Cf and thereby their multipolarities. The present measurements confirm the previous assignments of single-particle states in 251Cf. These include 1/2+[620], 0.0 keV; 7/2+[613], 106.30 keV; 3/2+[622], 177.59 keV; 11/2-[725], 370.47 keV; 9/2-[734], 433.91 keV; 5/2+[622], 543.98 keV; 1/2-[750], 632.0 keV; 9/2+[615], 683 keV; and 9/2+[604], 974.0 keV. A vibrational band was identified in previous studies at 981.4 keV and given an assignment of {7/2+[613]x2-}3/2-. Three new vibrational bands are identified in the present work at 942.5, 1086.5, and 1250.0 keV with tentative assignments {7/2+[613]x1-}5/2-,{7/2+[613]x1-}9/2-, and {7/2+[613]x0+}7/2+, respectively. A level was identified at 1185.5 keV with spin of 5/2 or 7/2 but it was not given any configuration assignment. Another level was identified at 1077.5 keV and given a spin of 9/2. Again, no configuration could be assigned to this level

  11. Mind Bomb-2 promotes U251 proliferation by regulating nuclear factor kappa B signaling pathways%Mind Bomb-2基因通过调控核因子κB信号通路促进 U251增殖的实验研究

    Institute of Scientific and Technical Information of China (English)

    张伯阳; 许重远

    2016-01-01

    Objective To study effects of Mind Bomb -2 ( MIB2 ) gene expression ’ s promotion on proliferation of U 251.Methods The expression of MIB2 protein in astrocyte and US251 was detected by Western blot.U251, U251 transduction with FLAG ( A 20 base coded marker protein ) plasmid and U251transfection with MIB2 -FLAG plasmid were divided into blank group , control group and test group correspondingly.The relative content of MIB 2 in three groups was detected by Western blotting .The MIB2 -FLAG plasmid was transfected to U251 cells.The real -time quantitative PCR was used to detect MIB 2 mRNA expression in U251.The expression of MIB2, I kappa B ( IkB) and nuclear factor kappa B ( NF-κB) was detected by Western blotting after transfection.MTT experiment was used to detect the proliferation of U251 cells after transfection.Results The relative content of MIB 2 in U251 cells increased by ( 17.04 ±2.91 ) compared with astrocytes.MIB2 mRNA in test group increased by ( 20.02 ±2.11 ) compared with control group.The expression of MIB 2 and NF-κB proteins in test group increased by ( 6.33 ±0.32 ) and ( 5.21 ±0.21 ) compared with control group, the expression of IkB in test group was attenuated by (0.43 ±0.04 ) compared with control group .Com-pared with both blank and control group , test group grew significantly ( P<0.001 ) .Conclusion MIB2 promoted U251 proliferation by regulating NF-κB signaling pathways.%目的:研究Mind Bomb-2(MIB2)的表达对脑胶质瘤细胞U251增殖的影响。方法用蛋白质印迹法检测正常星形胶质细胞和胶质瘤细胞U251和MIB2蛋白的表达情况。将U251细胞、转染FLAG (一种20个碱基编码的标记蛋白)质粒的U251细胞和转染MIB2-FLAG质粒的U251细胞对应分为空白组、对照组和实验组。以蛋白质印迹法检测上述3组中MIB2的相对含量;采用实时定量PCR技术检测MIB2 mRNA在3组U251细胞中的表达情况;以蛋白质印迹法检测转染后U251细胞中MIB2

  12. Localization of phosphorylated TrkA in carrier vesicles involved in its nuclear translocation in U251 cell line

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    A number of transmembrane receptors are targeted to the nucleus and convincingly localized therein. However, what remains a conundrum is how these cell-surface receptors end up in the nucleus. In this study, we reported that the transmembrane receptor phosphorylated TrkA was located in a series of carrier vesicles, including ring-like vesicles near the plasma membrane, large core vesicles and small dense core vesicles around the nuclei, as well as in the nucleus in human glioma cell line U251 using immunocytochemistry and immunofluorescence staining. Meanwhile, we also showed that small dense core vesicles budded from large core vesicles, and interacted with the nuclear envelope. Accordingly, our results suggested that such a series of membrane compartments might be involved in the pathway of nuclear translocation of the transmembrane receptor TrkA.

  13. Localization of phosphorylated TrkA in carrier vesicles involved in its nuclear translocation in U251 cell line

    Institute of Scientific and Technical Information of China (English)

    GONG AiHua; ZHANG ZhiJian; XIAO DeSheng; YANG Yong; WANG YongZhong; CHEN YongChang

    2007-01-01

    A number of transmembrane receptors are targeted to the nucleus and convincingly localized therein.However, what remains a conundrum is how these cell-surface receptors end up in the nucleus. In this study, we reported that the transmembrane receptor phosphorylated TrkA was located in a series of carrier vesicles, including ring-like vesicles near the plasma membrane, large core vesicles and small dense core vesicles around the nuclei, as well as in the nucleus in human glioma cell line U251 using immunocytochemistry and immunofluorescence staining. Meanwhile, we also showed that small dense core vesicles budded from large core vesicles, and interacted with the nuclear envelope. Accordingly,our results suggested that such a series of membrane compartments might be involved in the pathway of nuclear translocation of the transmembrane receptor TrkA.

  14. Two-dimensional Langevin modeling of fission dynamics of the excited compound nuclei 188Pt, 227Pa and 251Es

    Science.gov (United States)

    Eslamizadeh, H.

    2016-02-01

    A stochastic approach based on one- and two-dimensional Langevin equations is applied to calculate the pre-scission neutron multiplicity, fission probability, anisotropy of fission fragment angular distribution, fission cross section and the evaporation cross section for the compound nuclei 188Pt, 227Pa and 251Es in an intermediate range of excitation energies. The chaos weighted wall and window friction formula are used in the Langevin equations. The elongation parameter, c, is used as the first dimension and projection of the total spin of the compound nucleus onto the symmetry axis, K, considered as the second dimension in Langevin dynamical calculations. A constant dissipation coefficient of K, γK = 0.077(MeV zs)-1/2, is used in two-dimensional calculations to reproduce the above mentioned experimental data. Comparison of the theoretical results of the pre-scission neutron multiplicity, fission probability, fission cross section and the evaporation cross section with the experimental data shows that the results of two-dimensional calculations are in better agreement with the experimental data. Furthermore, it is shown that the two-dimensional Langevin equations together with a dissipation coefficient of K, γK = 0.077(MeV zs)-1/2, can satisfactorily reproduce the anisotropy of fission fragment angular distribution for the heavy compound nucleus 251Es. However, a larger value of γK = 0.250(MeV zs)-1/2 is needed to reproduce the anisotropy of fission fragment angular distribution for the lighter compound nucleus 227Pa.

  15. Common alleles at 6q25.1 and 1p11.2 are associated with breast cancer risk for BRCA1 and BRCA2 mutation carriers

    DEFF Research Database (Denmark)

    Antoniou, Antonis C; Kartsonaki, Christiana; Sinilnikova, Olga M;

    2011-01-01

    Two single nucleotide polymorphisms (SNPs) at 6q25.1, near the ESR1 gene, have been implicated in the susceptibility to breast cancer for Asian (rs2046210) and European women (rs9397435). A genome-wide association study in Europeans identified two further breast cancer susceptibility variants: rs...

  16. Common alleles at 6q25.1 and 1p11.2 are associated with breast cancer risk for BRCA1 and BRCA2 mutation carriers

    NARCIS (Netherlands)

    Antoniou, Antonis C.; Kartsonaki, Christiana; Sinilnikova, Olga M.; Soucy, Penny; McGuffog, Lesley; Healey, Sue; Lee, Andrew; Peterlongo, Paolo; Manoukian, Siranoush; Peissel, Bernard; Zaffaroni, Daniela; Cattaneo, Elisa; Barile, Monica; Pensotti, Valeria; Pasini, Barbara; Dolcetti, Riccardo; Giannini, Giuseppe; Putignano, Anna Laura; Varesco, Liliana; Radice, Paolo; Mai, Phuong L.; Greene, Mark H.; Andrulis, Irene L.; Glendon, Gord; Ozcelik, Hilmi; Thomassen, Mads; Gerdes, Anne-Marie; Kruse, Torben A.; Jensen, Uffe Birk; Crueger, Dorthe G.; Caligo, Maria A.; Laitman, Yael; Milgrom, Roni; Kaufman, Bella; Paluch-Shimon, Shani; Friedman, Eitan; Loman, Niklas; Harbst, Katja; Lindblom, Annika; Arver, Brita; Ehrencrona, Hans; Melin, Beatrice; Nathanson, Katherine L.; Domchek, Susan M.; Rebbeck, Timothy; Jakubowska, Ania; Lubinski, Jan; Gronwald, Jacek; Huzarski, Tomasz; Byrski, Tomasz; Cybulski, Cezary; Gorski, Bohdan; Osorio, Ana; Ramon y Cajal, Teresa; Fostira, Florentia; Andres, Raquel; Benitez, Javier; Hamann, Ute; Hogervorst, Frans B.; Rookus, Matti A.; Hooning, Maartje J.; Nelen, Marcel R.; van der Luijt, Rob B.; van Os, Theo A. M.; van Asperen, Christi J.; Devilee, Peter; Meijers-Heijboer, Hanne E. J.; Garcia, Encarna B. Gomez; Peock, Susan; Cook, Margaret; Frost, Debra; Platte, Radka; Leyland, Jean; Evans, D. Gareth; Lalloo, Fiona; Eeles, Ros; Izatt, Louise; Adlard, Julian; Davidson, Rosemarie; Eccles, Diana; Ong, Kai-ren; Cook, Jackie; Douglas, Fiona; Paterson, Joan; Kennedy, M. John; Miedzybrodzka, Zosia; Godwin, Andrew; Stoppa-Lyonnet, Dominique; Buecher, Bruno; Belotti, Muriel; Tirapo, Carole; Mazoyer, Sylvie; Barjhoux, Laure; Lasset, Christine; Leroux, Dominique; Faivre, Laurence; Bronner, Myriam; Prieur, Fabienne; Nogues, Catherine; Rouleau, Etienne; Pujol, Pascal; Coupier, Isabelle; Frenay, Marc; Hopper, John L.; Daly, Mary B.; Terry, Mary B.; John, Esther M.; Buys, Saundra S.; Yassin, Yosuf; Miron, Alexander; Goldgar, David; Singer, Christian F.; Tea, Muy-Kheng; Pfeiler, Georg; Dressler, Anne Catharina; Hansen, Thomas v. O.; Jonson, Lars; Ejlertsen, Bent; Barkardottir, Rosa Bjork; Kirchhoff, Tomas; Offit, Kenneth; Piedmonte, Marion; Rodriguez, Gustavo; Small, Laurie; Boggess, John; Blank, Stephanie; Basil, Jack; Azodi, Masoud; Toland, Amanda Ewart; Montagna, Marco; Tognazzo, Silvia; Agata, Simona; Imyanitov, Evgeny; Janavicius, Ramunas; Lazaro, Conxi; Blanco, Ignacio; Pharoah, Paul D. P.; Sucheston, Lara; Karlan, Beth Y.; Walsh, Christine S.; Olah, Edith; Bozsik, Aniko; Teo, Soo-Hwang; Seldon, Joyce L.; Beattie, Mary S.; van Rensburg, Elizabeth J.; Sluiter, Michelle D.; Diez, Orland; Schmutzler, Rita K.; Wappenschmidt, Barbara; Engel, Christoph; Meindl, Alfons; Ruehl, Ina; Varon-Mateeva, Raymonda; Kast, Karin; Deissler, Helmut; Niederacher, Dieter; Arnold, Norbert; Gadzicki, Dorothea; Schoenbuchner, Ines; Caldes, Trinidad; de la Hoya, Miguel; Nevanlinna, Heli; Aittomaki, Kristiina; Dumont, Martine; Chiquette, Jocelyne; Tischkowitz, Marc; Chen, Xiaoqing; Beesley, Jonathan; Spurdle, Amanda B.; Neuhausen, Susan L.; Ding, Yuan Chun; Fredericksen, Zachary; Wang, Xianshu; Pankratz, Vernon S.; Couch, Fergus; Simard, Jacques; Easton, Douglas F.; Chenevix-Trench, Georgia

    2011-01-01

    Two single nucleotide polymorphisms (SNPs) at 6q25.1, near the ESR1 gene, have been implicated in the susceptibility to breast cancer for Asian (rs2046210) and European women (rs9397435). A genome-wide association study in Europeans identified two further breast cancer susceptibility variants: rs112

  17. Common alleles at 6q25.1 and 1p11.2 are associated with breast cancer risk for BRCA1 and BRCA2 mutation carriers

    NARCIS (Netherlands)

    A.C. Antoniou (Antonis); C. Kartsonaki (Christiana); O. Sinilnikova (Olga); P. Soucy (Penny); L. McGuffog (Lesley); S. Healey (Sue); A. Lee (Andrew); P. Peterlongo (Paolo); S. Manoukian (Siranoush); B. Peissel (Bernard); D. Zaffaroni (D.); E. Cattaneo (Elisa); M. Barile (Monica); V. Pensotti (Valeria); B. Pasini (Barbara); R. Dolcetti (Riccardo); G. Giannini (Giuseppe); A.L. Putignano; L. Varesco (Liliana); P. Radice (Paolo); P.L. Mai (Phuong); M.H. Greene (Mark); I.L. Andrulis (Irene); G. Glendon (Gord); H. Ozcelik (Hilmi); M. Thomassen (Mads); A-M. Gerdes (Anne-Marie); T.A. Kruse (Torben); U.B. Jensen; D. Cruger (Dorthe); M.A. Caligo (Maria); Y. Laitman (Yael); R. Milgrom (Roni); B. Kaufman (Bella); S. Paluch-Shimon (Shani); E. Friedman (Eitan); N. Loman (Niklas); K. Harbst (Katja); A. Lindblom (Annika); B. Melin (Beatrice); K.L. Nathanson (Katherine); S.M. Domchek (Susan); R. Rebbeck (Timothy); A. Jakubowska (Anna); J. Lubinski (Jan); J. Gronwald (Jacek); T. Huzarski (Tomasz); T. Byrski (Tomasz); C. Cybulski (Cezary); B. Górski (Bohdan); A. Osorio (Ana); T.R. Cajal; F. Fostira (Florentia); R. Andres (Raquel); J. Benitez (Javier); U. Hamann (Ute); F.B.L. Hogervorst (Frans); M.A. Rookus (Matti); M.J. Hooning (Maartje); M.R. Nelen (Marcel); R.B. van der Luijt (Rob); T.A.M. van Os (Theo); C.J. van Asperen (Christi); P. Devilee (Peter); H. Meijers-Heijboer (Hanne); E.B.G. Garcia; S. Peock (Susan); M. Cook (Margaret); D. Frost; R. Platte (Radka); J. Leyland (Jean); D.G. Evans (Gareth); F. Lalloo (Fiona); R. Eeles (Rosalind); L. Izatt (Louise); R. Davidson (Rosemarie); D. Eccles (Diana); K.-R. Ong; F. Douglas (Fiona); J. Paterson (Joan); M.J. Kennedy (John); Z. Miedzybrodzka (Zosia); A.K. Godwin (Andrew); D. Stoppa-Lyonnet (Dominique); B. Buecher (Bruno); M. Belotti (Muriel); C. Tirapo (Carole); S. Mazoyer (Sylvie); L. Barjhoux (Laure); C. Lasset (Christine); D. Leroux (Dominique); L. Faivre (Laurence); M. Bronner (Myriam); F. Prieur (Fabienne); C. Nogues (Catherine); E. Rouleau (Etienne); P. Pujol (Pascal); I. Coupier (Isabelle); M. Frenay (Marc); J. Hopper (John); M.J. Daly (Mark); M-B. Terry (Mary-beth); E.M. John (Esther); S.S. Buys (Saundra); Y. Yassin (Yosuf); A. Miron (Alexander); D. Goldgar (David); C.F. Singer (Christian); M.-K. Tea; G. Pfeiler (Georg); C. Dressler (Catherina); T.V.O. Hansen (Thomas); L. Jønson (Lars); B. Ejlertsen (Bent); R.B. Barkardottir (Rosa); T. Kircchoff (Tomas); K. Offit (Kenneth); M. Piedmonte (Marion); G.C. Rodriguez (Gustavo); L. Small (Laurie); J.F. Boggess (John); S.V. Blank (Stephanie); J. Basil (Jack); M. Azodi (Masoud); A.E. Toland (Amanda); M. Montagna (Marco); S. Tognazzo (Silvia); S. Agata (Simona); E.N. Imyanitov (Evgeny); R. Janavicius (Ramunas); C. Lazaro (Conxi); I. Blanco (Ignacio); P.D.P. Pharoah (Paul); L. Sucheston (Lara); B.Y. Karlan (Beth); C.S. Walsh (Christine); E. Olah (Edith); A. Bozsik (Aniko); S.-H. Teo; J.L. Seldon (Joyce); M.S. Beattie (Mary); E.J. van Rensburg (Elizabeth); M.D. Sluiter (Michelle); O. Diez (Orland); R.K. Schmutzler (Rita); B. Wapenschmidt (Barbara); C. Engel (Christoph); A. Meindl (Alfons); I. Ruehl (Ina); R. Varon-Mateeva (Raymonda); K. Kast (Karin); H. Deissler (Helmut); D. Niederacher (Dieter); N. Arnold (Norbert); D. Gadzicki (Dorothea); I. Schönbuchner (Ines); T. Caldes (Trinidad); M. de La Hoya (Miguel); H. Nevanlinna (Heli); K. Aittomäki (Kristiina); M. Dumont (Martine); J. Chiquette (Jocelyne); M. Tischkowitz (Marc); G. Chenevix-Trench (Georgia); J. Beesley (Jonathan); A.B. Spurdle (Amanda); S.L. Neuhausen (Susan); Y.C. Ding (Yuan); Z. Fredericksen (Zachary); X. Wang (Xing); V.S. Pankratz (Shane); F.J. Couch (Fergus); J. Simard (Jacques); D.F. Easton (Douglas); G. Chenevix-Trench (Georgia); P. Karlsson (Per); M. Nordling (Margareta); A. Bergman (Annika); Z. Einbeigi (Zakaria); M. Stenmark-Askmalm (M.); S. Liedgren (Sigrun); Å. Borg (Åke); H. Olsson (Hans); U. Kristoffersson (Ulf); H. Jernström (H.); K. Henriksson (Karin); A. von Wachenfeldt (Anna); A. Liljegren (Annelie); G. Barbany-Bustinza (Gisela); J. Rantala (Johanna); H. Grönberg (Henrik); E.-L. Stattin; M. Emanuelsson (Monica); R.R. Brandell; N. Dahl (Niklas); S. Verhoef; M. Verheus (Martijn); L.v. Veer; F.E. van Leeuwen; J.M. Collee (Margriet); A.M.W. van den Ouweland (Ans); A. Jager; M.M.A. Tilanus-Linthorst (Madeleine); C.M. Seynaeve (Caroline); J.T. Wijnen (Juul); M.P. Vreeswijk (Maaike); R.A.E.M. Tollenaar (Rob); M.J. Ligtenberg (Marjolijn); N. Hoogerbrugge (Nicoline); M.G.E.M. Ausems (Margreet); C.M. Aalfs (Cora); J.J.P. Gille (Jan); Q. Waisfisz (Quinten); E.B. Gómez García (Encarna); C.E. van Roozendaal (Cees); M.J. Blok (Marinus); B. Caanen; J.C. Oosterwijk; A.H. van der Hout (Annemarie); M.J. Mourits; H.F. Vasen (Hans); H. Gregory (Helen); P.J. Morrison (Patrick); L. Jeffers (Lisa); T.J. Cole (Trevor); C. McKeown (Carole); J. Hoffman (Jonathan); A. Donaldson (Alan); S. Downing (Sarah); A. Taylor (Amy); A. Murray (Alexandra); M.T. Rogers (Mark); E. McCann (Emma); M.E. Porteous (Mary); S. Drummond (Sarah); C. Brewer (Carole); E. Kivuva (Emma); A. Searle (Anne); S. Goodman (Selina); K. Hill (Kathryn); V. Murday (Victoria); N. Bradshaw (Nicola); L. Snadden (Lesley); M. Longmuir (Mark); C. Watt (Catherine); S. Gibson (Sarah); E. Haque (Eshika); E. Tobias (Ed); A. Duncan (Alexis); C. Jacobs (Chris); C. Langman (Caroline); A. Whaite (Anna); H. Dorkins (Huw); J. Barwell (Julian); C. Chu (Chengbin); J. Miller (Julie); I.O. Ellis (Ian); C. Houghton (Catherine); L. Side (Lucy); A. Male (Alison); C. Berlin (Cheryl); J. Eason (Jacqueline); R. Collier (Rebecca); O. Claber (Oonagh); I. Jobson (Irene); L.J. Walker (Lisa); D. McLeod (Diane); D. Halliday (Dorothy); S. Durell (Sarah); B. Stayner (Barbara); S. Shanley; N. Rahman (Nazneen); R. Houlston (Richard); E. Bancroft (Elizabeth); L. D'Mello (Lucia); E. Page (Elizabeth); A. Ardern-Jones (Audrey); K. Kohut (Kelly); J. Wiggins (Jennifer); E. Castro (Elena); A. Mitra (Anita); L. Robertson (Lisa); O. Quarrell (Oliver); C. Bardsley (Cathryn); H. Ehrencrona (Hans); S.V. Hodgson (Shirley); D.E. Barton (David); S. Goff (Sheila); G. Brice (Glen); L. Winchester (Lizzie); C. Eddy (Charlotte); V. Tripathi (Vishakha); V. Attard (Virginia); A. Lucassen (Anneke); G. Crawford (Gillian); D. McBride (Donna); S. Smalley (Sarah); J.W. Adlard (Julian); B. Arver (Brita Wasteson)

    2011-01-01

    textabstractTwo single nucleotide polymorphisms (SNPs) at 6q25.1, near the ESR1 gene, have been implicated in the susceptibility to breast cancer for Asian (rs2046210) and European women (rs9397435). A genome-wide association study in Europeans identified two further breast cancer susceptibility var

  18. 25 CFR 1000.251 - What happens when a Tribe/Consortium is suspended for substantial failure to carry out the terms...

    Science.gov (United States)

    2010-04-01

    ... substantial failure to carry out the terms of an AFA without good cause and does not correct the failure... AMENDMENTS TO THE INDIAN SELF-DETERMINATION AND EDUCATION ACT Construction § 1000.251 What happens when a Tribe/Consortium is suspended for substantial failure to carry out the terms of an AFA without...

  19. 美洲商陆抗病毒蛋白对人神经胶质瘤细胞U251细胞增殖和凋亡的影响%Effects of recombinant pokeweed antiviral proteins from Phytolacca amercana on the proliferation and apoptosis of Human Gliomaous Cells U251

    Institute of Scientific and Technical Information of China (English)

    向莉; 李书剑; 张杰文

    2011-01-01

    目的:观察美洲商陆抗病毒蛋白(PAP)对入神经胶质瘤细胞U251细胞增殖和凋亡的影响.方法:采用MTT法检测0、20、40、60、80和100 mg/L PAP处理48 h以及40 mg/L PAP处理24、36、48和72 h对神经胶质瘤细胞U251细胞生长的影响;40 mg/L PAP处理U251细胞36 h后,采用荧光染色技术检测细胞凋亡,采用流式细胞仪检测U251细胞周期分布的影响;采用Northern blot和Western blot检测40 mg /L PAP处理24、48、72和96 h对U251细胞周期调控蛋白FasL和Fas的影响.结果:0、20、40、60、80和100 mg/L PAP处理48 h,U251细胞的增殖抑制率间的差异有统计学意义(F =284.560,P=0.007),40 mg/L PAP处理24、36、48和72 h,U251细胞的增殖抑制率间的差异有统计学意义(F=280.250,P=0.045).PAP促进U251细胞凋亡(t=106.350,P=0.007),PAP改变细胞周期分布,使G0+ G1期细胞比例增高,S期比例降低.PAP还可上调FasL蛋白表达,下调Fas蛋白表达.结论:PAP可改变细胞周期分布,影响细胞周期调控蛋白表达,并可诱导U251细胞凋亡,从而抑制细胞增殖.%Aim; To study the effects of pokeweed antiviral protein (PAP) on cell proliferation and apoptosis on U251 cells and to elucidate its molecular mechanism. Methods:The proportion of the periodic tumor cells were altered by 0,20, 40, 60,80, 100 mg/L PAP treated 48 h using MTT and Sub-G, curves were displayed at 40 mg/L PAP treated 36 h by flow cytometry analysis. With fluorescence staining assay to detect the U2S1 apoptosis. The mRNAs and protein expression of the FasL and Fas treated at 40 mg/L PAP was examined by Northern blot and Western blot. Results: PAP significantly suppressed U251 cell proliferation by 0,20,40,60,80,100 mg/L PAP treated 48 h(f = 284. 560,P = 0.007) or40ng/LPAP treated 24,36,48,72 h ( F = 28. 250,P =0. 045). The proportion of the periodic tumor cells were altered by PAP. PAP decreased the proportion of cells in S phase and increased the proportion of cells in Go/G, and G2/M

  20. The influence of the combined treatment with Vadimezan (ASA404 and taxol on the growth of U251 glioblastoma xenografts

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    Milanović Dušan

    2012-06-01

    Full Text Available Abstract Background One of the most important biological characteristics of Glioblastoma multiforme (GBM is high vascular density. Vadimezan (ASA404, DMXAA belongs to the class of small molecule vascular disrupting agents (VDA that cause disruption of established tumor vessels and subsequent tumor hemorrhagic necrosis. Its selective antivascular effect is mediated by intratumoral induction of several cytokines including tumor necrosis factor-α (TNF-α, granulocyte-colony-stimulating factor (G-CSF, interleukin 6 (IL-6 and macrophage inflammatory protein 1α (MIP-1α. Preclinical studies have demonstrated that ASA404 acts synergistically with taxanes. In this study, we investigated if treatment of mice bearing U251 human glioblastoma xenografts with ASA404 and taxol may be synergistic. Therapy response was evaluated by measuring changes in tumor size and metabolic activity using 18F-FDG PET (Fluorodeoxyglucose - positron emision tomography imaging. Methods U251 cells were inoculated s.c. in the right hind limb of NMRI-Foxn1nu athymic female nude mice. Animals were randomly assigned into 4 groups (7–9 animals/group for treatment: control, taxol, ASA404, and ASA404 plus taxol. The animals received either a single dose of taxol (10 mg/kg, ASA404 (27.5 mg/kg, or taxol (10 mg/kg plus ASA404 (27.5 mg/kg administered i.p.; ASA404 was administred 24 h after the treatment with taxol. 4 and 24 h after treatment with ASA404 (28 and 48 h hours after treatment with taxol 18 F-FDG PET scans were performed. Results The treatment with taxol did not affect the tumor growth in comparison to untreated controls. The treatment of animals with single dose ASA404 alone or in combination with taxol caused a significant delay in tumor growth. The combined treatment did not decrease the growth of the xenografts significantly more than ASA404 alone, but early changes in tumor 18 F-FDG uptake preceded subsequent growth inhibition. The tumor weights

  1. The influence of the combined treatment with Vadimezan (ASA404) and taxol on the growth of U251 glioblastoma xenografts

    International Nuclear Information System (INIS)

    One of the most important biological characteristics of Glioblastoma multiforme (GBM) is high vascular density. Vadimezan (ASA404, DMXAA) belongs to the class of small molecule vascular disrupting agents (VDA) that cause disruption of established tumor vessels and subsequent tumor hemorrhagic necrosis. Its selective antivascular effect is mediated by intratumoral induction of several cytokines including tumor necrosis factor-α (TNF-α), granulocyte-colony-stimulating factor (G-CSF), interleukin 6 (IL-6) and macrophage inflammatory protein 1α (MIP-1α). Preclinical studies have demonstrated that ASA404 acts synergistically with taxanes. In this study, we investigated if treatment of mice bearing U251 human glioblastoma xenografts with ASA404 and taxol may be synergistic. Therapy response was evaluated by measuring changes in tumor size and metabolic activity using 18F-FDG PET (Fluorodeoxyglucose - positron emision tomography) imaging. U251 cells were inoculated s.c. in the right hind limb of NMRI-Foxn1nu athymic female nude mice. Animals were randomly assigned into 4 groups (7–9 animals/group) for treatment: control, taxol, ASA404, and ASA404 plus taxol. The animals received either a single dose of taxol (10 mg/kg), ASA404 (27.5 mg/kg), or taxol (10 mg/kg) plus ASA404 (27.5 mg/kg) administered i.p.; ASA404 was administred 24 h after the treatment with taxol. 4 and 24 h after treatment with ASA404 (28 and 48 h hours after treatment with taxol) 18 F-FDG PET scans were performed. The treatment with taxol did not affect the tumor growth in comparison to untreated controls. The treatment of animals with single dose ASA404 alone or in combination with taxol caused a significant delay in tumor growth. The combined treatment did not decrease the growth of the xenografts significantly more than ASA404 alone, but early changes in tumor 18 F-FDG uptake preceded subsequent growth inhibition. The tumor weights, which were determined at the end of treatment, were lower in

  2. Spectroscopy of odd Z trans-fermium nuclei: the nuclear structure of Md{sup 251}; Spectroscopie des transfermiums impairs en proton: la structure du noyau de {sup 251}Md

    Energy Technology Data Exchange (ETDEWEB)

    Chatillon, A

    2005-10-01

    The objective of this thesis was to determine the structure of trans-fermium nuclei (Z 100) with odd proton number, which remained largely unexplored. These nuclei were produced in fusion-evaporation reactions with small cross sections below 1 {mu}b. The experimental methods of Recoil-Tagging and Recoil-Decay-Tagging were used for their identification. In order to identify the active orbitals in this mass region, {sup 255}Lr, {sup 251}M1d and {sup 247}Es nuclei have been studied by decay spectroscopy at the University of Jyvaskyla and at GANIL with the LISE spectrometer and the {alpha}-electron detector BEST coupled to four CLover detectors from the EXOGAM array. New states have been observed in each of the isotopes, and their configuration has been proposed. The collective properties were also studied in two experiments using prompt {gamma} and electron spectroscopy, combining the JUROGAM and SACRED arrays, respectively, with the recoil separator RITU and the GREAT spectrometer at its focal plane. A rotational band has been observed for the first time in a proton-odd trans-fermium nucleus. The interpretation of this collective structure is based on the theoretical HFB calculations. (author)

  3. Research on biological functions of U251 astroglioma cells infected with human herpesvirus type 6(HHV-6)%人类疱疹病毒6型感染U251细胞及其生物学功能的研究

    Institute of Scientific and Technical Information of China (English)

    郭一迪; 姚堃; 周锋; 李凌云; 茌静; 刘根焰; 陈云

    2010-01-01

    目的:研究人类疱疹病毒6型(HHV-6)感染人星形胶质瘤细胞U251,及感染后对其生物学功能的影响.方法:HHV-6感染U251细胞建立HHV-6体外感染模型,倒置显微镜观察细胞病变.PCR法检测HHV-6 u22基因.间接免疫荧光(IFA)检测HHV-6即刻早期蛋白(IEI)和晚期蛋白(gB)的表达.MTT法检测U251感染HHV-6后细胞增殖的改变.流式细胞术(FCM)检测U251细胞周期改变.结果:在感染3天后U251出现典型细胞病变,细胞肿胀增大数倍,透亮,呈多形性.PCR检测到HHV-6u22基因.IFA检测到HHV-6 IE1蛋白和异B蛋白的表达.MTT法显示HHV-6能增强U251细胞的增殖能力.FCM检测表明,HHV-6感染后U251细胞周期发生改变,感染组和对照组相比,前者G1期的细胞减少,S期和G2期细胞增多.结论:HHV-6能感染U251细胞引起细胞病变.促进细胞的增殖.使细胞周期发生改变.本研究结果提示人类疱疹病毒6型可能参与人神经胶质瘤的发生、发展,对其机制尚不清楚.

  4. Earth System Chemistry Integrated Modelling (ESCiMo with the Modular Earth Submodel System (MESSy, version 2.51

    Directory of Open Access Journals (Sweden)

    P. Jöckel

    2015-10-01

    Full Text Available With version 2.51 of the ECHAM/MESSy Atmospheric Chemistry (EMAC model three types of reference simulations as recommended by the Chemistry-Climate Model Initiative (CCMI have been performed: hindcast simulations (1950–2011, hindcast simulations with specified dynamics (1978–2013, i.e., nudged towards ERA-Interim reanalysis data, and combined hindcast and projection simulations (1950–2100. The manuscript summarises the updates of the model system and details the different model setups used, including the on-line calculated diagnostics. Simulations have been performed with two different nudging setups, with and without interactive tropospheric aerosol, and with and without a coupled ocean model. Two different vertical resolutions have been applied. The on-line calculated sources and sinks of reactive species are quantified and a first evaluation of the simulation results from a global perspective is provided as a quality check of the data. The focus is on the inter-comparison of the different model setups. The simulation data will become publicly available via CCMI and the CERA database of the German Climate Computing Centre (DKRZ. This manuscript is intended to serve as an extensive reference for further analyses of the ESCiMo simulations.

  5. [Intravenous regional sympathetic block with guanethidine. Retrospective study of 251 blocks of the upper limb in 68 patients].

    Science.gov (United States)

    Egloff, D V; Piaget, F

    1989-01-01

    68 patients have been treated by regional intravenous blocks with guanethidine. 251 blocks have been realized. The majority of the patients received 3 blocks of one week. Some of them got a second series of 3 blocks after a minimum interval of one month. The study included 3 groups of patients. The first group showed 12 patients in the first stage of Sudeck; the second group 22 patients in the second stage. The third group included 34 patients for which the indication to blockade had been a chronic painful stage (19 cases), an ankylosis (8 cases), a Dupuytren post-traumatic contracture (3 cases) a stage before the operation predisposing to dystrophy reflex (3 cases) and one case of hyperhidrosis of the hand. Considering the three groups it can be stated that the first one responded the best to the treatment with a success in 80% of the cases. This success confirms the necessity of intervening as soon as possible in case of an algoneurodystrophy. The third group reveals that this therapeutical method can be useful in other situations than the dystrophy reflexes. The indication herewith is more founded on a possible sympathetic origin of the troubles as on the comportment psycho-affective of the patient. PMID:2481373

  6. Earth System Chemistry integrated Modelling (ESCiMo) with the Modular Earth Submodel System (MESSy) version 2.51

    Science.gov (United States)

    Jöckel, Patrick; Tost, Holger; Pozzer, Andrea; Kunze, Markus; Kirner, Oliver; Brenninkmeijer, Carl A. M.; Brinkop, Sabine; Cai, Duy S.; Dyroff, Christoph; Eckstein, Johannes; Frank, Franziska; Garny, Hella; Gottschaldt, Klaus-Dirk; Graf, Phoebe; Grewe, Volker; Kerkweg, Astrid; Kern, Bastian; Matthes, Sigrun; Mertens, Mariano; Meul, Stefanie; Neumaier, Marco; Nützel, Matthias; Oberländer-Hayn, Sophie; Ruhnke, Roland; Runde, Theresa; Sander, Rolf; Scharffe, Dieter; Zahn, Andreas

    2016-03-01

    Three types of reference simulations, as recommended by the Chemistry-Climate Model Initiative (CCMI), have been performed with version 2.51 of the European Centre for Medium-Range Weather Forecasts - Hamburg (ECHAM)/Modular Earth Submodel System (MESSy) Atmospheric Chemistry (EMAC) model: hindcast simulations (1950-2011), hindcast simulations with specified dynamics (1979-2013), i.e. nudged towards ERA-Interim reanalysis data, and combined hindcast and projection simulations (1950-2100). The manuscript summarizes the updates of the model system and details the different model set-ups used, including the on-line calculated diagnostics. Simulations have been performed with two different nudging set-ups, with and without interactive tropospheric aerosol, and with and without a coupled ocean model. Two different vertical resolutions have been applied. The on-line calculated sources and sinks of reactive species are quantified and a first evaluation of the simulation results from a global perspective is provided as a quality check of the data. The focus is on the intercomparison of the different model set-ups. The simulation data will become publicly available via CCMI and the Climate and Environmental Retrieval and Archive (CERA) database of the German Climate Computing Centre (DKRZ). This manuscript is intended to serve as an extensive reference for further analyses of the Earth System Chemistry integrated Modelling (ESCiMo) simulations.

  7. Low dose rectal inoculation of rhesus macaques by SIV smE660 or SIVmac251 recapitulates

    Energy Technology Data Exchange (ETDEWEB)

    Hraber, Peter [Los Alamos National Laboratory; Giorgi, Elena E [Los Alamos National Laboratory; Keele, Brandon [UNIV OF ALABAMA; Li, Hui [UNIV OF ALABAMA; Learn, Gerald [UNIV OF ALABAMA

    2008-01-01

    We recently developed a novel strategy to identify transmitted HIV-1 genomes in acutely infected humans using single-genome amplification and a model of random virus evolution. Here, we used this approach to determine the molecular features of simian immunodeficiency virus (SIV) transmission in 18 experimentally infected Indian rhesus macaques. Animals were inoculated intrarectally (i.r.) or intravenously (i.v.) with stocks of SIVmac251 or SIVsmE660 that exhibited sequence diversity typical of early-chronic HIV-1 infection. 987 full-length SIV env sequences (median of 48 per animal) were determined from plasma virion RNA 1--5 wk after infection. i.r. inoculation was followed by productive infection by one or a few viruses (median 1; range 1--5) that diversified randomly with near starlike phylogeny and a Poisson distribution of mutations. Consensus viral sequences from ramp-up and peak viremia were identical to viruses found in the inocula or differed from them by only one or a few nucleotides, providing direct evidence that early plasma viral sequences coalesce to transmitted/founder viruses. i.v. infection was >2,000-fold more efficient than i.r. infection, and viruses transmitted by either route represented the full genetic spectra of the inocula. These findings identify key similarities in mucosal transmission and early diversification between SIV and HIV-1, and thus validate the SIV-macaque mucosal infection model for HIV-1 vaccine and microbicide research.

  8. Identification of a 251 gene expression signature that can accurately detect M. tuberculosis in patients with and without HIV co-infection.

    Directory of Open Access Journals (Sweden)

    Noor Dawany

    Full Text Available BACKGROUND: Co-infection with tuberculosis (TB is the leading cause of death in HIV-infected individuals. However, diagnosis of TB, especially in the presence of an HIV co-infection, can be limiting due to the high inaccuracy associated with the use of conventional diagnostic methods. Here we report a gene signature that can identify a tuberculosis infection in patients co-infected with HIV as well as in the absence of HIV. METHODS: We analyzed global gene expression data from peripheral blood mononuclear cell (PBMC samples of patients that were either mono-infected with HIV or co-infected with HIV/TB and used support vector machines to identify a gene signature that can distinguish between the two classes. We then validated our results using publically available gene expression data from patients mono-infected with TB. RESULTS: Our analysis successfully identified a 251-gene signature that accurately distinguishes patients co-infected with HIV/TB from those infected with HIV only, with an overall accuracy of 81.4% (sensitivity = 76.2%, specificity = 86.4%. Furthermore, we show that our 251-gene signature can also accurately distinguish patients with active TB in the absence of an HIV infection from both patients with a latent TB infection and healthy controls (88.9-94.7% accuracy; 69.2-90% sensitivity and 90.3-100% specificity. We also demonstrate that the expression levels of the 251-gene signature diminish as a correlate of the length of TB treatment. CONCLUSIONS: A 251-gene signature is described to (a detect TB in the presence or absence of an HIV co-infection, and (b assess response to treatment following anti-TB therapy.

  9. Earth System Chemistry integrated Modelling (ESCiMo) with the Modular Earth Submodel System (MESSy) version 2.51

    OpenAIRE

    Jöckel, Patrick; Tost, Holger; Pozzer, Andrea; Kunze, Markus; Kirner, Oliver; Brenninkmeijer, Carl A. M.; Brinkop, Sabine; Cai, Duy S.; Dyroff, Christoph; Eckstein, Johannes; Frank, Franziska; Garny, Hella; Gottschaldt, Klaus-Dirk; Graf, Phoebe; Grewe, Volker

    2016-01-01

    Abstract. Three types of reference simulations, as recommended by the Chemistry–Climate Model Initiative (CCMI), have been performed with version 2.51 of the European Centre for Medium-Range Weather Forecasts – Hamburg (ECHAM)/Modular Earth Submodel System (MESSy) Atmospheric Chemistry (EMAC) model: hindcast simulations (1950–2011), hindcast simulations with specified dynamics (1979–2013), i.e. nudged towards ERA-Interim reanalysis data, and combined hindcast and projection simulations (1950–...

  10. A clinical epidemiological study of 251 cases of amyotrophic lateral sclerosis in the south of Brazil Estudo clínico epidemiológico de 251 casos de esclerose lateral amiotrófica no sul do Brasil

    Directory of Open Access Journals (Sweden)

    Lineu Cesar Werneck

    2007-06-01

    Full Text Available OBJECTIVE: To study the clinical forms of amyotrophic lateral sclerosis (ALS and the possible presence of risk factors in order to verify if there is any difference between cases in Paraná, Brazil. METHOD: We studied 251 cases, all of which fulfilled the diagnosis criteria proposed in El Escorial (WFN. Between 1977 and 2004, 157 male and 94 female patients were examined. RESULTS: 220 cases were classified as ALS-Spinal Onset (ALS-SO, 24 as ALS-Bulbar Onset (ALS-BO and 7 as Familial ALS. The mean age at time of evaluation was 54.4±12.3 years, and symptoms had started 17.9±15.7months previously. In the group studied, statistical relationships were found between heavy occupations and males; previous surgeries and females; ALS-BO and dysphagia and dysarthria in females; and ALS-SO and males, cramps, weakness, muscle atrophy, hypertonia, increased deep tendon reflex and abnormal gait. CONCLUSION: The average age at time of evaluation was lower than that registered in the literature but similar to the Brazilian series. Domestic work and heavy occupations appear to be related to precocious perception of the symptoms by interference with daily functions. The socioeconomically higher classes seek medical care early. There was no relationship with exposure to toxic agents or trauma.OBJETIVO: Estudar as formas clínicas de esclerose lateral amiotrófica (ELA e possíveis fatores de risco, a fim de verificar se existem diferenças entre os casos do Paraná, Brasil. MÉTODO: Estudamos 251 casos entre 1977 e 2004, que preencheram os critérios propostos em El Escorial (WFN, sendo 157 do sexo masculino e 94 do feminino. RESULTADOS: Foram classificados como ELA de início espinhal (ELA-IE 220 casos, ELA de início bulbar (ELA-IB 24 casos e 7 casos como ELA familiar. A idade média na avaliação foi 54,4±12,3 anos cujos sintomas iniciaram 17,9 ±15,7 meses antes. Foram encontradas relações estatísticas entre ocupação que demandam esforços físicos com

  11. Functional simian immunodeficiency virus Gag-specific CD8+ intraepithelial lymphocytes in the mucosae of SIVmac251- or simian-human immunodeficiency virus KU2-infected macaques

    International Nuclear Information System (INIS)

    The vaginal and rectal mucosae are the first line of cellular immune defense to sexually transmitted human immunodeficiency virus type 1 (HIV-1) entry. Thus, intraepithelial lymphocytes (IELs) may be important in the immune response to HIV infection. Here we investigated whether functional IELs in mucosal compartments could be visualized by direct staining with a tetrameric complex specific for the simian immunodeficiency virus (SIV) immunodominant Gag epitope in either separated IEL cells or tissues of macaques infected with SIVmac251. Of the 15 Mamu-A*01-positive macaques studied here, eight were chronically infected with either SIVmac251 or simian-human immunodeficiency virus (SHIV) KU2 and the remaining seven were exposed mucosally to SIVmac251 and sacrificed within 48 h to assess the local immune response. Gag-specific CD8+ T-cells were found in separated IELs from the rectum, colon, jejunum, and vagina of most infected animals. Direct staining of tetramers also revealed their presence in intact tissue. These Gag-specific IELs expressed the activation marker CD69 and produced IFN-γ, suggesting an active immune response in this locale

  12. Rabies virus-based vaccines elicit neutralizing antibodies, poly-functional CD8+ T cell, and protect rhesus macaques from AIDS-like disease after SIVmac251 challenge

    Science.gov (United States)

    Faul, Elizabeth J.; Aye, Pyone P.; Papaneri, Amy B.; Pahar, Bapi; McGettigan, James P.; Schiro, Faith; Chervoneva, Inna; Montefiori, David C.; Lackner, Andrew A.; Schnell, Matthias J.

    2010-01-01

    Highly attenuated rabies virus (RV) vaccine vectors were evaluated for their ability to protect against highly pathogenic SIVmac251 challenge. Mamu-A*01 negative rhesus macaques were immunized in groups of four with either: RV expressing SIVmac239-GagPol, a combination of RV expressing SIVmac239-Env and RV expressing SIVmac239-GagPol, or with empty RV vectors. Eight weeks later animals received a booster immunization with a heterologous RV expressing the same antigens. At twelve weeks post-boost, all animals were challenged intravenously with 100 TCID50 of pathogenic SIVmac251-CX. Immunized macaques in both vaccine groups had 1.3–1.6-log fold decrease in viral set point compared to control animals. The GagPol/Env immunized animals also had a significantly lower peak viral load. When compared to control animals following challenge, vaccinated macaques had a more rapid induction of SIVmac251 neutralizing antibodies and of CD8+ T cell responses to various SIV epitopes. Moreover, vaccinated macaques better-maintained peripheral memory CD4+ T cells and were able to mount a poly-functional CD8+ T cell response in the mucosa. These findings indicate promise for RV-based vectors and have important implications for the development of an efficacious HIV vaccine. PMID:19879223

  13. Immune modulation through 4-1BB enhances SIV vaccine protection in non-human primates against SIVmac251 challenge.

    Directory of Open Access Journals (Sweden)

    Lauren A Hirao

    Full Text Available Costimulatory molecules play a central role in the development of cellular immunity. Understanding how costimulatory pathways can be directed to positively influence the immune response may be critical for the generation of an effective HIV vaccine. Here, we evaluated the ability of intravenous administration of a blocking monoclonal antibody (mAb directed against the negative costimulatory molecule CTLA-4, and an agonist mAb directed against the positive costimulatory molecule 4-1BB, either alone or in combination, to augment intramuscular SIV DNA immunizations. We then tested the ability these of these responses to impact a high-dose SIVmac251 challenge. Following immunization, the groups infused with the anti-4-1BB mAb exhibited enhanced IFN-γ responses compared to the DNA vaccine only group. Interestingly, although CTLA-4 blockade alone did not enhance IFN-γ responses it did increase the proliferative capacity of the CD4(+ and CD8(+ T cells. The combination of both mAbs enhanced the magnitude of the polyfunctional CD8(+ T cell response. Following challenge, the group that received both mAbs exhibited a significant, ∼2.0 log, decrease in plasma viral load compared to the naïve group the included complete suppression of viral load in some animals. Furthermore, the use of the CTLA-4 blocking antibody resulted in significantly higher viral loads during chronic infection compared to animals that received the 4-1BB mAb, likely due to the higher CD4(+ T cell proliferative responses which were driven by this adjuvant following immunization. These novel studies show that these adjuvants induce differential modulation of immune responses, which have dramatically different consequences for control of SIV replication, suggesting important implications for HIV vaccine development.

  14. Characterization of Mycobacterium leprae Genotypes in China--Identification of a New Polymorphism C251T in the 16S rRNA Gene.

    Science.gov (United States)

    Yuan, Youhua; Wen, Yan; You, Yuangang; Xing, Yan; Li, Huanying; Weng, Xiaoman; Wu, Nan; Liu, Shuang; Zhang, Shanshan; Zhang, Wenhong; Zhang, Ying

    2015-01-01

    Leprosy continues to be prevalent in some mountainous regions of China, and genotypes of leprosy strains endemic to the country are not known. Mycobacterium lepromatosis is a new species that was discovered in Mexico in 2008, and it remains unclear whether this species exists in China. Here, we conducted PCR- restriction fragment length polymorphism (RFLP) analysis to classify genotypes of 85 DNA samples collected from patients from 18 different provinces. All 171 DNA samples from skin biopsies of leprosy patients were tested for the presence of Mycobacterium leprae and Mycobacterium lepromatosis by amplifying the 16S rRNA gene using nested PCR, followed by DNA sequencing. The new species M. lepromatosis was not found among the 171 specimens from leprosy patients in 22 provinces in China. However, we found three SNP genotypes among 85 leprosy patients. A mutation at C251T in the 16S rRNA gene was found in 76% of the strains. We also found that the strains that showed the 16S rRNA C251T mutation belonged to SNP type 3, whereas strains without the point mutation belonged to SNP type 1. The SNP type 3 leprosy strains were observed in patients from both the inner and coastal regions of China, but the SNP type 1 strains were focused only in the coastal region. This indicated that the SNP type 3 leprosy strains were more prevalent than the SNP type 1 strains in China. In addition, the 16S rRNA gene sequence mutation at C251T also indicated a difference in the geographical distribution of the strains. To our knowledge, this is the first report of a new polymorphism in 16S rRNA gene in M. leprae in China. Our findings shed light on the prevalent genotypes and provide insight about leprosy transmission that are important for leprosy control in China. PMID:26196543

  15. Characterization of Mycobacterium leprae Genotypes in China--Identification of a New Polymorphism C251T in the 16S rRNA Gene.

    Directory of Open Access Journals (Sweden)

    Youhua Yuan

    Full Text Available Leprosy continues to be prevalent in some mountainous regions of China, and genotypes of leprosy strains endemic to the country are not known. Mycobacterium lepromatosis is a new species that was discovered in Mexico in 2008, and it remains unclear whether this species exists in China. Here, we conducted PCR- restriction fragment length polymorphism (RFLP analysis to classify genotypes of 85 DNA samples collected from patients from 18 different provinces. All 171 DNA samples from skin biopsies of leprosy patients were tested for the presence of Mycobacterium leprae and Mycobacterium lepromatosis by amplifying the 16S rRNA gene using nested PCR, followed by DNA sequencing. The new species M. lepromatosis was not found among the 171 specimens from leprosy patients in 22 provinces in China. However, we found three SNP genotypes among 85 leprosy patients. A mutation at C251T in the 16S rRNA gene was found in 76% of the strains. We also found that the strains that showed the 16S rRNA C251T mutation belonged to SNP type 3, whereas strains without the point mutation belonged to SNP type 1. The SNP type 3 leprosy strains were observed in patients from both the inner and coastal regions of China, but the SNP type 1 strains were focused only in the coastal region. This indicated that the SNP type 3 leprosy strains were more prevalent than the SNP type 1 strains in China. In addition, the 16S rRNA gene sequence mutation at C251T also indicated a difference in the geographical distribution of the strains. To our knowledge, this is the first report of a new polymorphism in 16S rRNA gene in M. leprae in China. Our findings shed light on the prevalent genotypes and provide insight about leprosy transmission that are important for leprosy control in China.

  16. Variants Located Upstream of CHRNB4 on Chromosome 15q25.1 Are Associated with Age at Onset of Daily Smoking and Habitual Smoking

    OpenAIRE

    Kapoor, Manav; Wang, Jen-Chyong; Bertelsen, Sarah; Bucholz, Kathy; Budde, John P; Hinrichs, Anthony; Agrawal, Arpana; Brooks, Andrew; Chorlian, David; Dick, Danielle; Hesselbrock, Victor; Foroud, Tatiana; Kramer, John; Kuperman, Samuel; Manz, Niklas

    2012-01-01

    Several genome-wide association and candidate gene studies have linked chromosome 15q24–q25.1 (a region including the CHRNA5-CHRNA3-CHRNB4 gene cluster) with alcohol dependence, nicotine dependence and smoking-related illnesses such as lung cancer and chronic obstructive pulmonary disease. To further examine the impact of these genes on the development of substance use disorders, we tested whether variants within and flanking the CHRNA5-CHRNA3-CHRNB4 gene cluster affect the transition to dail...

  17. Spectroscopic and structural elucidation of merocyanine dye 2,5-[1-metyl-4-[2-(4-hydroxyphenyl)ethenyl)]piridinium]-hexane tetraphenylborate. Aggregation processes

    Science.gov (United States)

    Koleva, Bojidarka B.; Stoyanov, Stanimir; Kolev, Tsonko; Petkov, Ivan; Spiteller, Michael

    2008-12-01

    Structural and spectroscopic elucidation of merocyanine dye, 2,5-[1-metyl-4-[2-(4-hydroxyphenyl)ethenyl)]piridinium]-hexane tetraphenylborate, is performed in gas and condense phase by means of solution and solid-state conventional and linear-polarized IR-spectroscopy of oriented colloids in nematic liquid crystal suspension, UV-vis and fluorescence methods, HPLC MS/MS tandem and ESI mass spectrometry, 1H, 13C and 1H- 1H COSY NMR, TGV and DSC methods. Quantum chemical DFT calculations are performed for structural optimization and spectroscopic properties prediction.

  18. Rabies virus-based vaccines elicit neutralizing antibodies, poly-functional CD8+ T cell, and protect rhesus macaques from AIDS-like disease after SIVmac251 challenge

    OpenAIRE

    Elizabeth J. Faul; Aye, Pyone P.; Papaneri, Amy B.; Pahar, Bapi; McGettigan, James P.; Schiro, Faith; Chervoneva, Inna; Montefiori, David C.; Lackner, Andrew A.; Schnell, Matthias J.

    2009-01-01

    Highly attenuated rabies virus (RV) vaccine vectors were evaluated for their ability to protect against highly pathogenic SIVmac251 challenge. Mamu-A*01 negative rhesus macaques were immunized in groups of four with either: RV expressing SIVmac239-GagPol, a combination of RV expressing SIVmac239-Env and RV expressing SIVmac239-GagPol, or with empty RV vectors. Eight weeks later animals received a booster immunization with a heterologous RV expressing the same antigens. At twelve weeks post-bo...

  19. High Frequency of Virus-Specific CD8+ T Cells in the Central Nervous System of Macaques Chronically Infected with Simian Immunodeficiency Virus SIVmac251

    OpenAIRE

    Moniuszko, Marcin; Brown, Charlie; Pal, Ranajit; Tryniszewska, Elzbieta; Tsai, Wen-Po; Hirsch, Vanessa M.; Franchini, Genoveffa

    2003-01-01

    Infection with human immunodeficiency virus or simian immunodeficiency virus (SIV) induces virus-specific CD8+ T cells that traffic to lymphoid and nonlymphoid tissues. In this study, we used Gag-specific tetramer staining to investigate the frequency of CD8+ T cells in peripheral blood and the central nervous system of Mamu-A*01-positive SIV-infected rhesus macaques. Most of these infected macaques were vaccinated prior to SIVmac251 exposure. The frequency of Gag181-189 CM9 tetramer-positive...

  20. Influence of the CB1 receptor antagonist, AM 251, on the regional haemodynamic effects of WIN-55212-2 or HU 210 in conscious rats

    OpenAIRE

    Gardiner, S. M.; March, J. E.; Kemp, P. A.; Bennett, T.

    2002-01-01

    In conscious, freely-moving, male, Sprague-Dawley rats, the regional haemodynamic responses to the synthetic cannabinoids, WIN-55212-2 and HU 210, were compared. The possible involvement of cannabinoid, CB1-receptors, or β2-adrenoceptors in the responses to WIN-55212-2 and HU 210 were investigated using the CB1-receptor antagonist, AM 251, or the β2-adrenoceptor antagonist, ICI 118551, respectively.Both WIN-55212-2 (150 μg kg−1) and HU 210 (100 μg kg−1) had pressor, renal, and mesenteric vaso...

  1. Comparison of the transcriptional activity of the long terminal repeats of simian immunodeficiency viruses SIVmac251 and SIVmac239 in T-cell lines and macrophage cell lines.

    OpenAIRE

    Anderson, M G; Clements, J E

    1991-01-01

    The U3 regions of the long terminal repeats (LTRs) of simian immunodeficiency viruses SIVmac251 and SIVmac239 were analyzed for basal transcriptional activity and for interaction with cellular factors in the T-cell line HUT-78 and the monocyte/macrophage cell line U937. A number of 5' deletions and mutations were made in the U3 regions of the two LTRs, and these constructs were placed upstream of a plasmid containing the bacterial chloramphenicol acetyltransferase reporter gene. The nucleotid...

  2. In vitro study on siRNA integrin α_vβ_3 gene expression inhibiting glioma U251 cells proliferation%siRNA干扰整合素α_vβ_3表达抑制脑胶质瘤细胞U251增殖的实验研究

    Institute of Scientific and Technical Information of China (English)

    赵天书; 王策; 王雪峰; 董白晶; 孙庆喜; 张尧; 李利; 刘晓谦

    2010-01-01

    目的 探索特异性siRNA靶向干扰恶性胶质瘤U251细胞中INTα_vβ_3表达后对细胞生长的作用.方法 将INTα_vβ_3特异性siRNA经脂质体(LipofectamineTM~(2000))转染U251细胞.RT-PCR、免疫细胞化学和Western blot法检测INTα_vβ_3蛋白表达.MTT检测干扰后细胞增殖变化.结果 INTα_vβ_3特异siRNA对U251细胞增殖有明显抑制作用.siRNA组、脂质体组与对照组各时间点的结果 差异有统计学意义.免疫细胞化学对照组和脂质体组均见INTα_vβ_3呈绿色表达,siRNA组表达降低.Western blot法和RT-PCR结果 证实转染特异siRNA后的细胞在蛋白及mRNA水平均能抑制INTα_vβ_3的表达.结论 特异性siRNA可干扰INTα_vβ_3在脑胶质瘤U251细胞中的表达并抑制细胞增殖.%Objective Explore the specificity of siRNA interference targeting malignant glioma U251 cells, INTα_vβ_3 expression of cell growth after the role. Method U251 cells were transfected with INT α_vβ_3 specific siRNA by lipofectamine(LipefectamineTM~(2000)). RT-PCR, immune ceils staining and Western blot were used to determine the expression of INTα_vβ_3. Cell proliferation after the detection of changes in interference was tested by MTT. Results INTα_vβ_3 specific siRNA shown to inhibit U251 cell proliferation. There were statistical significance between siRNA group, liposome group and the control group at different time points. Green INTα_vβ_3 expression was found in control group and lipesome groups, and reduced in siRNA group. Western blotting and RT-PCR results shown that the expression of integrin could be inhibited by α_vβ_3 transfection in the protein and mRNA level Conclusions Integrin-specific siRNA INTα_vβ_3 can inhibit the U251 glioma cell proliferation.

  3. 慢病毒介导的 RWDD3沉默对人胶质瘤 U251细胞增殖和侵袭的影响%Effects of lentivirus-mediated RWDD3 silencing on proliferation and inva-sion of human glioma U251 cells

    Institute of Scientific and Technical Information of China (English)

    范阳华; 祝新根; 吕世刚; 吴淼经; 柴毅; 叶敏华; 肖兵; 吴雷

    2015-01-01

    AIM:To investigate the effect of RWDD3 gene silencing on the biological characteristics of human glioma U251 cells.METHODS: A lentiviral vector expressing RWDD3 shRNA was constructed and transfeeted into the U251 cells.The expression of RWDD3 at mRNA and protein levels was detected by real-time PCR and Western blot , re-spectively .The cell activity was determined by MTT assay .The colony formation ability was detected by the colony forma-tion assay .The cell proliferation ability was detected by BrdU incorporation assay .The cell invasion and migration were evaluated by Transwell assay .Flow cytometry was used to monitor the changes of cell cycle distribution and apoptosis .RE-SULTS:Recombinant lentivirus was successfully transfected into U 251 cells.Compared with the cells transfected with the scrambled shRNA and control cells, the cell activity, colony formation ability, and the invasive and migratory activities were inhibited, the cell cycle was arrested in G 0/G1 phase, and the apoptosis was increased in the U 251 cells transfected with RWDD3 shRNA ( P <0.05 ) .CONCLUSION: RWDD3 plays a vital role in proliferation and invasion of glioma cells.It may serve as a potential target of gene therapy for glioma .%目的:探讨沉默RWDD3表达对人胶质瘤U251细胞生物学特征的影响。方法:构建靶向RWDD3的shRNA重组慢病毒并转染U251细胞,通过real-time PCR和Western blot 在mRNA及蛋白水平鉴定转染结果。MTT法检测转染后细胞的细胞活力;平板克隆实验检测克隆形成能力;BrdU实验检测细胞增殖能力;Transwell 实验检测细胞迁移和侵袭能力的变化;流式细胞术检测细胞周期及细胞凋亡的改变。结果: real-time PCR和Western blot结果均表明成功建立稳定沉默RWDD3的U251细胞株。与空白对照组及阴性对照组比较,转染RWDD3-shR-NA组的细胞活力和克隆形成能力降低;侵袭及迁移能力均下降,穿膜细胞

  4. H{sub 2} reduction of surface oxides on Pd-based membrane model systems – The case of Pd(1 0 0) and Pd{sub 75}Ag{sub 25}(1 0 0)

    Energy Technology Data Exchange (ETDEWEB)

    Fernandes, V.R. [Department of Physics, Norwegian University of Science and Technology, NO-7491 Trondheim (Norway); Gustafson, J. [Division of Synchrotron Radiation Research, Lund University, Box 117, SE-221 00 Lund (Sweden); Farstad, M.H.; Walle, L.E. [Department of Physics, Norwegian University of Science and Technology, NO-7491 Trondheim (Norway); Blomberg, S.; Lundgren, E. [Division of Synchrotron Radiation Research, Lund University, Box 117, SE-221 00 Lund (Sweden); Venvik, H.J. [Department of Chemical Engineering, Norwegian University of Science and Technology, NO-7491 Trondheim (Norway); Borg, A., E-mail: anne.borg@ntnu.no [Department of Physics, Norwegian University of Science and Technology, NO-7491 Trondheim (Norway)

    2014-09-15

    Highlights: • H{sub 2} reduction of the surface oxide is significantly slower for Pd{sub 75}Ag{sub 25}(1 0 0) compared to Pd(1 0 0). • The reduction behavior shows complex temperature dependence, not well described by Avrami kinetics. • Oxygen spillover effects during the surface oxide reduction are observed for Pd(1 0 0). • For Pd(1 0 0) the observed reduction rate is rather independent of temperature. • For Pd{sub 75}Ag{sub 25}(1 0 0) the reduction rate displays a non-monotonic variation with temperature. - Abstract: Reduction of the (√(5)×√(5))R27° surface oxide on Pd(1 0 0) and Pd{sub 75}Ag{sub 25}(1 0 0) surfaces by H{sub 2} has been studied using high-resolution photoelectron spectroscopy in situ at H{sub 2} pressures 5 × 10{sup −9} mbar and 5 × 10{sup −8} mbar and selected temperatures in the range 30 °C to 170 °C. The reduction is slower on Pd{sub 75}Ag{sub 25}(1 0 0) compared to Pd(1 0 0) for all temperatures and pressures investigated. For Pd(1 0 0), the surface oxide reduction rate is rather independent of temperature, while for Pd{sub 75}Ag{sub 25}(1 0 0) a non-monotonic variation is observed. As indicated by kinetic analysis, the complex reduction behavior is not well described by Avrami kinetics. Oxygen spillover effects contribute to this picture for Pd(1 0 0), while surface compositional effects appear to dominate the performance for Pd{sub 75}Ag{sub 25}(1 0 0). These findings may have implications for understanding the oxidation, reduction and hydrogen transport properties of Pd–Ag membranes.

  5. Chronic Δ(9)-Tetrahydrocannabinol Administration Reduces IgE(+)B Cells but Unlikely Enhances Pathogenic SIVmac251 Infection in Male Rhesus Macaques of Chinese Origin.

    Science.gov (United States)

    Wei, Qiang; Liu, Li; Cong, Zhe; Wu, Xiaoxian; Wang, Hui; Qin, Chuan; Molina, Patricia; Chen, Zhiwei

    2016-09-01

    Delta9-tetrahydrocannabinol (Δ(9)-THC) is the major psychoactive component of the cannabis plant. Δ(9)-THC has been used in the active ingredient of Marinol as an appetite stimulant for AIDS patients. Its impact on progression of HIV-1 infection, however, remains debatable. Previous studies indicated that Δ(9)-THC administration enhanced HIV-1 infection in huPBL-SCID mice but seemingly decreased early mortality in simian immunodeficiency virus (SIV) infected male Indian-derived rhesus macaques. Here, we determine the chronic effect of Δ(9)-THC administration using 0.32 mg/kg or placebo (PBO), i.m., twice daily for 428 days on SIVmac251 infected male Chinese-derived rhesus macaques. Sixteen animals were divided into four study groups: Δ(9)-THC(+)SIV(+), Δ(9)-THC(+)SIV(-), PBO/SIV(+) and PBO/SIV(-) (n = 4/group). One-month after daily Δ(9)-THC or PBO administrations, macaques in groups one and three were challenged intravenously with pathogenic SIVmac251/CNS, which was isolated from the brain of a Chinese macaque with end-staged neuroAIDS. No significant differences in peak and steady state plasma viral loads were seen between Δ(9)-THC(+)SIV(+) and PBO/SIV(+) macaques. Regardless of Δ(9)-THC, all infected macaques displayed significant drop of CD4/CD8 T cell ratio, loss of CD4(+) T cells and higher persistent levels of Ki67(+)CD8(+) T cells compared with uninfected animals. Moreover, long-term Δ(9)-THC treatment reduced significantly the frequency of circulating IgE(+)B cells. Only one Δ(9)-THC(+)SIV(+) macaque died of simian AIDS with paralyzed limbs compared with two deaths in the PBO/SIV(+) group during the study period. These findings indicate that chronic Δ(9)-THC administration resulted in reduction of IgE(+)B cells, yet it unlikely enhanced pathogenic SIVmac251/CNS infection in male Rhesus macaques of Chinese origin. PMID:27109234

  6. MicroRNA-21 inhibitor sensitizes human glioblastoma cells U251 (PTEN-mutant) and LN229 (PTEN-wild type) to taxol

    International Nuclear Information System (INIS)

    Substantial data indicate that the oncogene microRNA 21 (miR-21) is significantly elevated in glioblastoma multiforme (GBM) and regulates multiple genes associated with cancer cell proliferation, apoptosis, and invasiveness. Thus, miR-21 can theoretically become a target to enhance the chemotherapeutic effect in cancer therapy. So far, the effect of downregulating miR-21 to enhance the chemotherapeutic effect to taxol has not been studied in human GBM. Human glioblastoma U251 (PTEN-mutant) and LN229 (PTEN wild-type) cells were treated with taxol and the miR-21 inhibitor (in a poly (amidoamine) (PAMAM) dendrimer), alone or in combination. The 50% inhibitory concentration and cell viability were determined by the MTT assay. The mechanism between the miR-21 inhibitor and the anticancer drug taxol was analyzed using the Zheng-Jun Jin method. Annexin V/PI staining was performed, and apoptosis and the cell cycle were evaluated by flow cytometry analysis. Expression of miR-21 was investigated by RT-PCR, and western blotting was performed to evaluate malignancy related protein alteration. IC(50) values were dramatically decreased in cells treated with miR-21 inhibitor combine with taxol, to a greater extent than those treated with taxol alone. Furthermore, the miR-21 inhibitor significantly enhanced apoptosis in both U251 cells and LN229 cells, and cell invasiveness was obviously weakened. Interestingly, the above data suggested that in both the PTEN mutant and the wild-type GBM cells, miR-21 blockage increased the chemosensitivity to taxol. It is worth noting that the miR-21 inhibitor additively interacted with taxol on U251cells and synergistically on LN229 cells. Thus, the miR-21 inhibitor might interrupt the activity of EGFR pathways, independently of PTEN status. Meanwhile, the expression of STAT3 and p-STAT3 decreased to relatively low levels after miR-21 inhibitor and taxol treatment. The data strongly suggested that a regulatory loop between miR-21 and STAT3 might

  7. 美洲商陆抗病毒蛋白在毕赤酵母中的表达及其诱发人神经胶质瘤细胞U251凋亡的研究%Cloning and Expression of Pokeweed Antiviral Protein Gene from Phytolacca amercana in Pichia pastoris and the Study of Apoptosis of Human Neuroglioma Cells U251 Induced by Recombinant PAP

    Institute of Scientific and Technical Information of China (English)

    向莉; 胡亚梅; 张杰文

    2011-01-01

    Objective To clone the pokeweed anti-viral protein (PAP) gene, to express it in Pichia pas-troris, and to study the inhibitory effect of PAP on U251 in vitro. Methods The cDNA sequence encoding PAP was cloned by Real-time PCR from Phytolacca amercana. The recombinant PAP was subcloned into the expression vector pPICZaA and expressed in Pichia pastroris GSM 5 after methanol induction. SDS-PAGE analysis showed that the expressed PAP existed in the yeast culture supernatant. The drug cytotoxicity to U251 cells was assessed using MTT assay and the obvious apoptotic nuclei of the tumor cells detected using the method of single cell gel electrophoresis. Results The full-length PAP gene was cloned. The recombinant expression plasmid pPICZaA-PAP was constructed successfully. SDS-PAGE analysis showed that the relative molecular mass (M) of the recombinant protein was about 35 kDa. The degradation of the genome of the apoptotic cells induced by PAP was detected using the method of single cell gel electrophoresis. PAP possessed very high ability to inhibit the growth of U251. The anti-tumor activities (IC50) to U251 cells of PAP was 81. 0 pg/mL. Conclusion PAP could be a potent anti-tumor candidate for inhibiting the growth of U251 and inducing its apoptosis.%目的 研究美洲商陆抗病毒蛋白(pokeweed antiviral protein,PAP)基因的克隆表达,进而研究其诱发人神经胶质瘤细胞U251凋亡.方法 利用RT-PCR技术克隆PAP基因,构建PAP毕赤酵母表达质粒pPIC-ZaA-PAP并导入毕赤酵母Pichia pastoris CS115.SDS-PAGE检测PAP的分泌表达.采用镍离子亲合层析纯化PAP,并通过单细胞凝胶电泳和MTT检测其抑制人神经胶质瘤细胞U251的生长.结果 分泌表达的PAP融合蛋白分子量约为35/kD,纯化的PAP在体外能诱发人神经胶质瘤细胞U251凋亡.PAP对U251半数抑制浓度(IC50)为81.0μg/mL,通过单细胞凝胶电泳,能看到明显的慧星尾,表明PAP引起了神经胶质瘤细

  8. Low dose rectal inoculation of rhesus macaques by SIV SME660 or SIV MAC251 recapitulates human mucosal infection by HIV-1

    Energy Technology Data Exchange (ETDEWEB)

    Koraber, Bette [Los Alamos National Laboratory; Perelson, Alan [Los Alamos National Laboratory; Hraber, Peter [Los Alamos National Laboratory; Giorgi, E [Los Alamos National Laboratory; Bhattacharya, T [Los Alamos National Laboratory

    2009-01-01

    Recently, we developed a novel approach to the identification of transmitted or early founder HIV -1 genomes in acutely infected humans based on single genome amplification and sequencing. Here we tested this approach in 18 acutely infected Indian rhesus macaques to determine the molecular features of SIV transmission. Animals were inoculated intrarectally (IR) or intravenously (IV) with stocks of SIVmac251 or SIVsmE660 that exhibited sequence diversity typical of early-chronic HIV -1 infection. 987 full-length SIV env sequences (median of 48 per animal) were determined from plasma virion RNA one to five weeks after infection. IR inoculation was followed by productive infection by one or few viruses (median 1; range 1-5) that diversified randomly with near star-like phylogeny and a Poisson distribution of mutations. Consensus viral sequences from ramp-up and peak viremia were identical to viruses found in the inocula or differed from them by only one or few nuc1eotides, providing direct evidence that early plasma viral sequences coalesce to transmitted/founder virus( es). IV infection was approximately 10,000-fold more efficient than IR infection, and viruses transmitted by either route represented the full genetic spectra of the inocula. These findings identify key similarities in mucosal transmission and early diversification between SIV and HIV -1.

  9. Association of a single nucleotide polymorphism at 6q25.1,rs2046210, with endometrial cancer risk among Chinese women

    Institute of Scientific and Technical Information of China (English)

    Guoliang Li; Qiuyin Cai; Yong-Bing Xiang; Regina Courtney; Jia-Rong Cheng; Bo Huang; Ji-Rong Long; Hui Cai; Wei Zheng; Xiao-Ou Shu

    2011-01-01

    A recent genome-wide association study identified a new susceptibility locus for breast cancer, rs2046210, which is a single nucleotide polymorphism (SNP) located upstream of the estrogen receptor α (ESR1) gene on chromosome 6q25.1. Given that endometrial cancer shares many risk factors with breast cancer and both are related to estrogen exposure and that rs2046210 is in close proximity to the ESR1 gene, we evaluated the association of SNP rs2046210 with endometrial cancer risk among 953 cases and 947 controls in a population-based, case-control study conducted in Shanghai, China. Logistic regression models were used to derive odds ratios (ORs) and 95% confidence intervals (95% Cis) after adjusting for potential confounders. We found that the A allele of rs2046210, linked to an increased risk of breast cancer, was associated with increased but not statistically significant risk of endometrial cancer (OR = 1.16, 95% CI = 0.96-1.41 for the GA and AA genotypes compared with the GG genotype); the association was stronger among post-menopausal women (OR = 1.28, 95% CI = 1.00-1.65). The association tended to be stronger among women with higher or longer estrogen exposure than among women with relatively lower or shorter exposure to estrogen. Our study suggests that rs2046210 may play a role in the etiology of endometrial cancer. Additional studies are needed to confirm our findings.

  10. "Perfect Zoom System" which enables both a zoom ratio of 25:1 and a high-resolution in stereo microscope

    Science.gov (United States)

    Miyake, Norio; Mizuta, Masahiro; Shinada, Nobuhiro; Nakayama, Hiroaki; Ohuchi, Yumiko

    2015-09-01

    A stereomicroscope can stereoscopically observe an object with protrusions and recesses as if the object were viewed by both eyes. Such stereomicroscopes use an optical system to create two slightly different viewing angles of an object. The different images are both enlarged and viewed through two eyepieces. Each of the observation optical systems includes a variable magnification mechanism which is called a zoom lens system. In recent years, a demand for stereomicroscopes that can observe a wide variable magnification range has been increasing along with the diversification of applications. However, there are no stereomicroscope zoom lenses with both a high resolution and a large zoom ratio. We developed the "Perfect Zoom System" which can reduce the light flux diameter going through the objective lens in the low-power state. In the "Perfect Zoom System", the zoom lens groups move along not only the optical axes but also perpendicular to the axes. Therefore, the objective lens could be made smaller by decentering the G2 and G3 group lenses in zoom lens group. We achieved a high resolution and a zoom ratio of 25:1.

  11. A novel autosomal recessive non-syndromic hearing impairment locus (DFNB47) maps to chromosome 2p25.1-p24.3.

    Science.gov (United States)

    Hassan, Muhammad Jawad; Santos, Regie Lyn P; Rafiq, Muhammad Arshad; Chahrour, Maria H; Pham, Thanh L; Wajid, Muhammad; Hijab, Nadine; Wambangco, Michael; Lee, Kwanghyuk; Ansar, Muhammad; Yan, Kai; Ahmad, Wasim; Leal, Suzanne M

    2006-01-01

    Hereditary hearing impairment (HI) displays extensive genetic heterogeneity. Autosomal recessive (AR) forms of prelingual HI account for approximately 75% of cases with a genetic etiology. A novel AR non-syndromic HI locus (DFNB47) was mapped to chromosome 2p25.1-p24.3, in two distantly related Pakistani kindreds. Genome scan and fine mapping were carried out using microsatellite markers. Multipoint linkage analysis resulted in a maximum LOD score of 4.7 at markers D2S1400 and D2S262. The three-unit support interval was bounded by D2S330 and D2S131. The region of homozygosity was found within the three-unit support interval and flanked by markers D2S2952 and D2S131, which corresponds to 13.2 cM according to the Rutgers combined linkage-physical map. This region contains 5.3 Mb according to the sequence-based physical map. Three candidate genes, KCNF1, ID2 and ATP6V1C2 were sequenced, and were found to be negative for functional sequence variants. PMID:16261342

  12. Optical simulations for the S3 project - Super separator spectrometer - gamma-electron coincidence spectroscopy of a transfermium nucleus: the 251Md101

    International Nuclear Information System (INIS)

    In analogy with the atomic closed shells giving rise to the stability and high ionisation energies of noble gases, nuclear physics also has its magic numbers of protons and neutrons which enhance nuclear structure stability. Knowledge of the structure of doubly-magic nuclei, both proton and neutron numbers, is crucial to parameterize theoretical models. The discovery of the next and ultimate magic numbers will provide a strong constraint on the many predictions. These two numbers are like the centre coordinates of an area of enhanced stability of the nuclear chart, well known as 'island of stability'. These superheavy nuclei only exist due to pure quantum shell effects. My thesis work deals with two distinct, but complementary, aspects of fundamental physics with the common goal of studying these extreme mass nuclei structure. The first part corresponds to the development of a next generation instrument for nuclear physics to allow synthesis and spectroscopy studies of superheavy nuclei: the Super Separator Spectrometer S3. This project will be installed at SPIRAL2 (GANIL) and has been approved by the French Research National Agency (ANR) within the EQUIPEX framework. It has been designed to take advantage of the high intensity heavy ion beam from the LINAC, giving access to a wide range of physical programs. The second part corresponds to the preparation, realisation and analysis of an experiment on 251-Mendelevium in which the very first prompt gamma-electron coincidence spectroscopy was performed for a transfermium nuclei. (author)

  13. Response of a simian immunodeficiency virus (SIVmac251 to raltegravir: a basis for a new treatment for simian AIDS and an animal model for studying lentiviral persistence during antiretroviral therapy

    Directory of Open Access Journals (Sweden)

    Greenhouse Jack

    2010-03-01

    Full Text Available Abstract Background In this study we successfully created a new approach to ART in SIVmac251 infected nonhuman primates. This drug regimen is entirely based on drugs affecting the pre-integration stages of replication and consists of only two nucleotidic/nucleosidic reverse transcriptase inhibitors (Nt/NRTIs and raltegravir, a promising new drug belonging to the integrase strand transfer inhibitor (INSTI class. Results In acutely infected human lymphoid CD4+ T-cell lines MT-4 and CEMx174, SIVmac251 replication was efficiently inhibited by raltegravir, which showed an EC90 in the low nanomolar range. This result was confirmed in primary macaque PBMCs and enriched CD4+ T cell fractions. In vivo monotherapy with raltegravir for only ten days resulted in reproducible decreases in viral load in two different groups of animals. When emtricitabine (FTC and tenofovir (PMPA were added to treatment, undetectable viral load was reached in two weeks, and a parallel increase in CD4 counts was observed. In contrast, the levels of proviral DNA did not change significantly during the treatment period, thus showing persistence of this lentiviral reservoir during therapy. Conclusions In line with the high conservation of the three main amino acids Y143, Q148 and N155 (responsible for raltegravir binding and molecular docking simulations showing similar binding modes of raltegravir at the SIVmac251 and HIV-1 IN active sites, raltegravir is capable of inhibiting SIVmac251 replication both in tissue culture and in vivo. This finding may help to develop effective ART regimens for the simian AIDS model entirely based on drugs adopted for treatment in humans. This ART-treated AIDS nonhuman primate model could be employed to find possible strategies for virus eradication from the body.

  14. Tracking past changes in lake-water phosphorus with a 251-lake calibration dataset in British Columbia: tool development and application in a multiproxy assessment of eutrophication and recovery in Osoyoos Lake, a transboundary lake in Western North America

    OpenAIRE

    Cumming, Brian F.; Laird, Kathleen R.; Gregory-Eaves, Irene; Simpson, Kyle G.; Sokal, Michael A.; Nordin, Rick N.; Walker, Ian R.

    2015-01-01

    Recently, there has been an active discussion about the potential and challenges of tracking past lake-water trophic state using paleolimnological methods. Herein, we present analyses of the relationship between modern-day diatom assemblages from the surface sediments of 251 fresh-water lakes from British Columbia and contemporary limnological variables. Total phosphorus (TP) was significantly related to the modern distribution of diatom assemblages. The large size of this new calibration dat...

  15. Rabies virus-based vaccines elicit neutralizing antibodies, poly-functional CD8+ T cell, and protect rhesus macaques from AIDS-like disease after SIV(mac251) challenge.

    Science.gov (United States)

    Faul, Elizabeth J; Aye, Pyone P; Papaneri, Amy B; Pahar, Bapi; McGettigan, James P; Schiro, Faith; Chervoneva, Inna; Montefiori, David C; Lackner, Andrew A; Schnell, Matthias J

    2009-12-11

    Highly attenuated rabies virus (RV) vaccine vectors were evaluated for their ability to protect against highly pathogenic SIV(mac251) challenge. Mamu-A*01 negative rhesus macaques were immunized in groups of four with either: RV expressing SIV(mac239)-GagPol, a combination of RV expressing SIV(mac239)-Env and RV expressing SIV(mac239)-GagPol, or with empty RV vectors. Eight weeks later animals received a booster immunization with a heterologous RV expressing the same antigens. At 12 weeks post-boost, all animals were challenged intravenously with 100 TCID(50) of pathogenic SIV(mac251-CX). Immunized macaques in both vaccine groups had 1.3-1.6-log-fold decrease in viral set point compared to control animals. The GagPol/Env immunized animals also had a significantly lower peak viral load. When compared to control animals following challenge, vaccinated macaques had a more rapid induction of SIV(mac251) neutralizing antibodies and of CD8(+) T cell responses to various SIV epitopes. Moreover, vaccinated macaques better maintained peripheral memory CD4(+) T cells and were able to mount a poly-functional CD8(+) T cell response in the mucosa. These findings indicate promise for RV-based vectors and have important implications for the development of an efficacious HIV vaccine. PMID:19879223

  16. Oxidation-reduction properties of americium, curium, berkelium, californium, einsteinium and fermium, and thermodynamic consequences for the 5f series

    International Nuclear Information System (INIS)

    The amalgamation of 5f elements from Am to Fm has been studied by using 241Am, 244Cm, 249Bk, 249Cf, 252Cf, 253Es, 254Es, 252Fm and 255Fm with two electrochemical methods, radiocoulometry and radiopolarography, perfectly adapted to investigate extremely diluted solutions when the concentration of electroactive species is as low as 10-16M. The theory of radiocoulometry has been developed in the general cases of reversible and irreversible electrode process. It has been used to interpret the experimental data on the kinetic curves of amalgamation, and to estimate the standard rate constant of the electrode process in complexing medium (citric). On the other hand the radiopolarographic method has been applied to study the mechanism of reduction at the dropping mercury electrode of cations M3+ in aqueous medium to the metal M with formation of amalgam. The results are exploited into two directions: 1- Acquisition of some data concerning the oxidation-reduction properties of elements from Am to Fm. Therefore the standard electrode E0 [M(III-0)] potentials for Bk, Cf and Es, and the standard electrode E0 [M(II-0)] potential for Fm are estimated and the relative stability of each oxidation state (from II to VII) of 5f elements is discussed; 2- Acquisition of unknown thermodynamic data on transcalifornium elements. Correlations between 4f and 5f elements are precised and some divergences appear for the second half of 4f and 5f series (i.e. for 65<=Z<=71 and 97<=Z<=103)

  17. Evaluation of thermal neutron cross-sections and resonance integrals of protactinium, americium, curium, and berkelium isotopes

    International Nuclear Information System (INIS)

    Data on the thermal neutron fission and capture cross-sections as well as their corresponding resonance integrals are reviewed and analysed. The data are classified according to the form of neutron spectra under investigation. The weighted mean values of the cross-sections and resonance integrals for every type of neutron spectra were adopted as evaluated data. (author). 87 refs, 2 tabs

  18. Variants located upstream of CHRNB4 on chromosome 15q25.1 are associated with age at onset of daily smoking and habitual smoking.

    Directory of Open Access Journals (Sweden)

    Manav Kapoor

    Full Text Available Several genome-wide association and candidate gene studies have linked chromosome 15q24-q25.1 (a region including the CHRNA5-CHRNA3-CHRNB4 gene cluster with alcohol dependence, nicotine dependence and smoking-related illnesses such as lung cancer and chronic obstructive pulmonary disease. To further examine the impact of these genes on the development of substance use disorders, we tested whether variants within and flanking the CHRNA5-CHRNA3-CHRNB4 gene cluster affect the transition to daily smoking (individuals who smoked cigarettes 4 or more days per week in a cross sectional sample of adolescents and young adults from the COGA (Collaborative Study of the Genetics of Alcoholism families. Subjects were recruited from families affected with alcoholism (either as a first or second degree relative and the comparison families. Participants completed the SSAGA interview, a comprehensive assessment of alcohol and other substance use and related behaviors. Using the Quantitative trait disequilibrium test (QTDT significant association was detected between age at onset of daily smoking and variants located upstream of CHRNB4. Multivariate analysis using a Cox proportional hazards model further revealed that these variants significantly predict the age at onset of habitual smoking among daily smokers. These variants were not in high linkage disequilibrium (0.28

  19. Location and dynamics of the immunodominant CD8 T cell response to SIVΔnef immunization and SIVmac251 vaginal challenge.

    Directory of Open Access Journals (Sweden)

    Arun K Sasikala-Appukuttan

    Full Text Available Live-attenuated SIV vaccines (LAVs have been the most effective to date in preventing or partially controlling infection by wild-type SIV in non-human primate models of HIV-1 transmission to women acting by mechanisms of protection that are not well understood. To gain insights into mechanisms of protection by LAVs that could aid development of effective vaccines to prevent HIV-1 transmission to women, we used in situ tetramer staining to determine whether increased densities or changes in the local distribution of SIV-specific CD8 T cells correlated with the maturation of SIVΔnef vaccine-induced protection prior to and after intra-vaginal challenge with wild-type SIVmac251. We evaluated the immunodominant Mamu-A1*001:01/Gag (CM9 and Mamu-A1*001:01/Tat (SL8 epitope response in genital and lymphoid tissues, and found that tetramer+ cells were present at all time points examined. In the cervical vaginal tissues, most tetramer+ cells were distributed diffusely throughout the lamina propria or co-localized with other CD8 T cells within lymphoid aggregates. The distribution and densities of the tetramer+ cells at the portal of entry did not correlate with the maturation of protection or change after challenge. Given these findings, we discuss the possibility that changes in other aspects of the immune system, including the quality of the resident population of virus-specific effector CD8 T cells could contribute to maturation of protection, as well as the potential for vaccine strategies that further increase the size and quality of this effector population to prevent HIV-1 transmission.

  20. ESR1 is co-expressed with closely adjacent uncharacterised genes spanning a breast cancer susceptibility locus at 6q25.1.

    Directory of Open Access Journals (Sweden)

    Anita K Dunbier

    2011-04-01

    Full Text Available Approximately 80% of human breast carcinomas present as oestrogen receptor α-positive (ER+ve disease, and ER status is a critical factor in treatment decision-making. Recently, single nucleotide polymorphisms (SNPs in the region immediately upstream of the ER gene (ESR1 on 6q25.1 have been associated with breast cancer risk. Our investigation of factors associated with the level of expression of ESR1 in ER+ve tumours has revealed unexpected associations between genes in this region and ESR1 expression that are important to consider in studies of the genetic causes of breast cancer risk. RNA from tumour biopsies taken from 104 postmenopausal women before and after 2 weeks treatment with an aromatase (oestrogen synthase inhibitor was analyzed on Illumina 48K microarrays. Multiple-testing corrected Spearman correlation revealed that three previously uncharacterized open reading frames (ORFs located immediately upstream of ESR1, C6ORF96, C6ORF97, and C6ORF211 were highly correlated with ESR1 (Rs =  0.67, 0.64, and 0.55 respectively, FDR<1 × 10(-7. Publicly available datasets confirmed this relationship in other groups of ER+ve tumours. DNA copy number changes did not account for the correlations. The correlations were maintained in cultured cells. An ERα antagonist did not affect the ORFs' expression or their correlation with ESR1, suggesting their transcriptional co-activation is not directly mediated by ERα. siRNA inhibition of C6ORF211 suppressed proliferation in MCF7 cells, and C6ORF211 positively correlated with a proliferation metagene in tumours. In contrast, C6ORF97 expression correlated negatively with the metagene and predicted for improved disease-free survival in a tamoxifen-treated published dataset, independently of ESR1. Our observations suggest that some of the biological effects previously attributed to ER could be mediated and/or modified by these co-expressed genes. The co-expression and function of these genes may be

  1. Mucosal B Cells Are Associated with Delayed SIV Acquisition in Vaccinated Female but Not Male Rhesus Macaques Following SIVmac251 Rectal Challenge.

    Directory of Open Access Journals (Sweden)

    Iskra Tuero

    2015-08-01

    Full Text Available Many viral infections, including HIV, exhibit sex-based pathogenic differences. However, few studies have examined vaccine-related sex differences. We compared immunogenicity and protective efficacy of monomeric SIV gp120 with oligomeric SIV gp140 in a pre-clinical rhesus macaque study and explored a subsequent sex bias in vaccine outcome. Each immunization group (16 females, 8 males was primed twice mucosally with replication-competent Ad-recombinants encoding SIVsmH4env/rev, SIV239gag and SIV239nefΔ1-13 and boosted twice intramuscularly with SIVmac239 monomeric gp120 or oligomeric gp140 in MF59 adjuvant. Controls (7 females, 5 males received empty Ad and MF59. Up to 9 weekly intrarectal challenges with low-dose SIVmac251 were administered until macaques became infected. We assessed vaccine-induced binding, neutralizing, and non-neutralizing antibodies, Env-specific memory B cells and plasmablasts/plasma cells (PB/PC in bone marrow and rectal tissue, mucosal Env-specific antibodies, and Env-specific T-cells. Post-challenge, only one macaque (gp140-immunized remained uninfected. However, SIV acquisition was significantly delayed in vaccinated females but not males, correlated with Env-specific IgA in rectal secretions, rectal Env-specific memory B cells, and PC in rectal tissue. These results extend previous correlations of mucosal antibodies and memory B cells with protective efficacy. The gp140 regimen was more immunogenic, stimulating elevated gp140 and cyclic V2 binding antibodies, ADCC and ADCP activities, bone marrow Env-specific PB/PC, and rectal gp140-specific IgG. However, immunization with gp120, the form of envelope immunogen used in RV144, the only vaccine trial to show some efficacy, provided more significant acquisition delay. Further over 40 weeks of follow-up, no gp120 immunized macaques met euthanasia criteria in contrast to 7 gp140-immunized and 2 control animals. Although males had higher binding antibodies than females, ADCC

  2. The LLNL Heavy Element Facility -- Facility Management, Authorization Basis, and Readiness Assessment Lessons Learned in the Heavy Element Facility (B251) Transition from Category II Nuclear Facility to Radiological Facility

    International Nuclear Information System (INIS)

    This paper presents Facility Management, Readiness Assessment, and Authorization Basis experience gained and lessons learned during the Heavy Element Facility Risk Reduction Program (RRP). The RRP was tasked with removing contaminated glove boxes, radioactive inventory, and contaminated ventilation systems from the Heavy Element Facility (B251) at Lawrence Livermore National Laboratory (LLNL). The RRP was successful in its goal in April 2005 with the successful downgrade of B251 from a Category II Nuclear Facility to a Radiological Facility. The expertise gained and the lessons learned during the planning and conduct of the RRP included development of unique approaches in work planning/work control (''Expect the unexpected and confirm the expected'') and facility management. These approaches minimized worker dose and resulted in significant safety improvements and operational efficiencies. These lessons learned can help similar operational and management activities at other sites, including facilities restarting operations or new facility startup. B251 was constructed at LLNL to provide research areas for conducting experiments in radiochemistry using transuranic elements. Activities at B251 once included the preparation of tracer sets associated with the underground testing of nuclear devices and basic research devoted to a better understanding of the chemical and nuclear behavior of the transuranic elements. Due to the age of the facility, even with preventative maintenance, facility safety and experimental systems were deteriorating. A variety of seismic standards were used in the facility design and construction, which encompassed eight building increments constructed over a period of 26 years. The cost to bring the facility into compliance with the current seismic and other requirements was prohibitive, and simply maintaining B251 as a Category II nuclear facility posed serious cost considerations under a changing regulatory environment. Considering the high

  3. The LLNL Heavy Element Facility -- Facility Management, Authorization Basis, and Readiness Assessment Lessons Learned in the Heavy Element Facility (B251) Transition from Category II Nuclear Facility to Radiological Facility

    Energy Technology Data Exchange (ETDEWEB)

    Mitchell, M; Anderson, B; Brown, E; Gray, L

    2006-04-10

    This paper presents Facility Management, Readiness Assessment, and Authorization Basis experience gained and lessons learned during the Heavy Element Facility Risk Reduction Program (RRP). The RRP was tasked with removing contaminated glove boxes, radioactive inventory, and contaminated ventilation systems from the Heavy Element Facility (B251) at Lawrence Livermore National Laboratory (LLNL). The RRP was successful in its goal in April 2005 with the successful downgrade of B251 from a Category II Nuclear Facility to a Radiological Facility. The expertise gained and the lessons learned during the planning and conduct of the RRP included development of unique approaches in work planning/work control (''Expect the unexpected and confirm the expected'') and facility management. These approaches minimized worker dose and resulted in significant safety improvements and operational efficiencies. These lessons learned can help similar operational and management activities at other sites, including facilities restarting operations or new facility startup. B251 was constructed at LLNL to provide research areas for conducting experiments in radiochemistry using transuranic elements. Activities at B251 once included the preparation of tracer sets associated with the underground testing of nuclear devices and basic research devoted to a better understanding of the chemical and nuclear behavior of the transuranic elements. Due to the age of the facility, even with preventative maintenance, facility safety and experimental systems were deteriorating. A variety of seismic standards were used in the facility design and construction, which encompassed eight building increments constructed over a period of 26 years. The cost to bring the facility into compliance with the current seismic and other requirements was prohibitive, and simply maintaining B251 as a Category II nuclear facility posed serious cost considerations under a changing regulatory environment

  4. Increased radiosensitivity and radiothermosensitivity of human pancreatic MIA PaCa-2 and U251 glioblastoma cell lines treated with the novel Hsp90 inhibitor NVP-HSP990

    International Nuclear Information System (INIS)

    Heat shock Protein 90 (Hsp90) is a molecular chaperone that folds, stabilizes, and functionally regulates many cellular proteins involved in oncogenic signaling and in the regulation of radiosensitivity. It is upregulated in response to stress such a heat. Hyperthermia is a potent radiosensitizer, but induction of Hsp90 may potentially limit its efficacy. Our aim was to investigate whether the new Hsp90 inhibitor NVP-HSP990 increases radiosensitivity, thermosensitivity and radiothermosensitivity of human tumor cell lines. U251 glioblastoma and MIA PaCa-2 pancreatic carcinoma cells were used. To determine clonogenic survival, colony forming assays were performed. Cell viability and proliferation were assesed by Trypan blue staining. Cell cycle and apoptosis analyses were performed by flow cytometry. DAPI staining was used to detect mitotic catastrophe. NVP-HSP990 increased the thermosensitivity, radiosensitivity and radio-thermosensitivity of both cell lines in clonogenic assays. 72 hours after irradiation with 4 Gy, a significant reduction in cell number associated with considerable G2/M acumulation and mitotic catastrophe as well as cell death by apoptosis/necrosis was observed. Treatment with NVP-HSP990 strongly sensitized U251 and MIA PaCa-2 cells to hyperthermia and ionizing radiation or combination thereof through augmentation of G2/M arrest, mitotic catastrophe and associated apoptosis

  5. TNF-alpha and IL-8: serum levels and gene polymorphisms (-308G>A and -251A>T) are associated with classical biomarkers and medical history in children with sickle cell anemia.

    Science.gov (United States)

    Cajado, C; Cerqueira, B A V; Couto, F D; Moura-Neto, J P; Vilas-Boas, W; Dorea, M J; Lyra, I M; Barbosa, C G; Reis, M G; Goncalves, M S

    2011-11-01

    Sickle cell anemia (SCA) is a disorder characterized by a heterogeneous clinical outcome. In the present study, we investigated the associations between Tumor Necrosis Factor-alpha (TNF-alpha) -308G>A and Interleukin 8 (IL-8) -251A>T gene polymorphisms, medical history and classical biomarkers in children with steady-state SCA. In total, 210 SCA patients aged 2-21 years and 200 healthy controls were studied. Gene polymorphisms, betaS-globin haplotypes and a 3.7-kb deletion in alpha2-thalassemia (α2-thal3.7 kb) were investigated by PCR/RFLP analysis, and cytokine levels were determined by ELISA. Splenomegaly (p=.032) was more prevalent among children younger than 5 years of age. The A allele of the TNF-alpha -308G>A gene polymorphism and the presence of α2-thal3.7 kb were associated with an increase risk of splenic sequestration events (p=.001; p=.046), while the T allele of the IL-8 -251A>T gene polymorphism was considered to be a protective factor for splenomegaly events (p=.032). Moreover, the A allele of the TNF-alpha -308G>A gene polymorphism was associated with high TNF-alpha levels (p=.021), and the hemoglobin F and hemoglobin S haplotypes were correlated with serum levels of IL-8. The logistic regression analysis showed significant effects of the TNF-alpha and IL-8 gene polymorphisms, beta(S)-globin gene haplotypes and α2-thal3.7 kb on the occurrence of splenic sequestration events. Our study emphasizes that the identification of new genetic and immunological biomarkers and their associations with classical markers is an important strategy to elucidate the underlying causes of different SCA phenotypes and their effects on patient outcome. PMID:21802960

  6. Simian immunodeficiency virus (SIV envelope quasispecies transmission and evolution in infant rhesus macaques after oral challenge with uncloned SIVmac251: increased diversity is associated with neutralizing antibodies and improved survival in previously immunized animals

    Directory of Open Access Journals (Sweden)

    Earl Patricia

    2005-02-01

    Full Text Available Abstract Background Oral infection of infant macaques with simian immunodeficiency virus (SIV is a useful animal model to test interventions to reduce postnatal HIV transmission via breast-feeding. We previously demonstrated that immunization of infant rhesus macaques with either modified vaccinia virus Ankara (MVA expressing SIV Gag, Pol and Env, or live-attenuated SIVmac1A11 resulted in lower viremia and longer survival compared to unimmunized controls after oral challenge with virulent SIVmac251 (Van Rompay et al., J. Virology 77:179–190, 2003. Here we evaluate the impact of these vaccines on oral transmission and evolution of SIV envelope variants. Results Limiting dilution analysis of SIV RNA followed by heteroduplex mobility assays of the V1–V2 envelope (env region revealed two major env variants in the uncloned SIVmac251 inoculum. Plasma sampled from all infants 1 week after challenge contained heterogeneous SIV env populations including one or both of the most common env variants in the virus inoculum; no consistent differences in patterns of env variants were found between vaccinated and unvaccinated infants. However, SIV env variant populations diverged in most vaccinated monkeys 3 to 5 months after challenge, in association with the development of neutralizing antibodies. Conclusions These patterns of viral envelope diversity, immune responses and disease course in SIV-infected infant macaques are similar to observations in HIV-infected children, and underscore the relevance of this pediatric animal model. The results also support the concept that neonatal immunization with HIV vaccines might modulate disease progression in infants infected with HIV by breast-feeding.

  7. Differences in time of virus appearance in the blood and virus-specific immune responses in intravenous and intrarectal primary SIVmac251 infection of rhesus macaques; a pilot study

    Directory of Open Access Journals (Sweden)

    Washington Parks Robyn

    2001-07-01

    Full Text Available Abstract Background HIV-I can be transmitted by intravenous inoculation of contaminated blood or blood product or sexually through mucosal surfaces. Here we performed a pilot study in the SIVmac251 macaque model to address whether the route of viral entry influences the kinetics of the appearance and the size of virus-specific immune in different tissue compartments. Methods For this purpose, of 2 genetically defined Mamu-A*01-positive macaques, 1 was exposed intravenously and the other intrarectally to the same SIVmac251 viral stock and virus-specific CD8+ T-cells were measured within the first 12 days of infection in the blood and at day 12 in several tissues following euthanasia. Results Virus-specific CD8+ T-cell responses to Gag, Env, and particularly Tat appeared earlier in the blood of the animal exposed by the mucosal route than in the animal exposed intravenously. The magnitude of these virus-specific responses was consistently higher in the systemic tissues and GALT of the macaque exposed by the intravenous route, suggesting a higher viral burden in the tissues as reflected by the faster appearance of virus in plasma. Differences in the ability of the virus-specific CD8+ T-cells to respond in vitro to specific peptide stimulation were also observed and the greatest proliferative ability was found in the GALT of the animal infected by the intrarectal route. Conclusions These data may suggest that the natural mucosal barrier may delay viral spreading. The consequences of this observation, if confirmed in studies with a larger number of animals, may have implications in vaccine development.

  8. Prior DNA immunization enhances immune response to dominant and subdominant viral epitopes induced by a fowlpox-based SIVmac vaccine in long-term slow-progressor macaques infected with SIVmac251

    International Nuclear Information System (INIS)

    A therapeutic vaccine for individuals infected with HIV-1 and treated with antiretroviral therapy (ART) should be able to replenish virus-specific CD4+ T-cells and broaden the virus-specific CD8+ T-cell response in order to maintain CD8+ T-cell function and minimize viral immune escape after ART cessation. Because a combination of DNA and recombinant poxvirus vaccine modalities induces high levels of virus-specific CD4+ T-cell response and broadens the cytolytic activity in naive macaques, we investigated whether the same results could be obtained in SIVmac251-infected macaques. The macaques studied here were long-term nonprogressors that naturally contained viremia but were nevertheless treated with a combination of antiviral drugs to assess more carefully the effect of vaccination in the context of ART. The combination of a DNA expressing the gag and pol genes (DNA-SIV-gp) of SIVmac239 followed by a recombinant fowlpox expressing the same SIVmac genes (FP-SIV-gp) was significantly more immunogenic than two immunizations of FP-SIV-gp in SIVmac251-infected macaques treated with ART. The DNA/FP combination significantly expanded and broadened Gag-specific T-cell responses measured by tetramer staining, ELISPOT, and intracellular cytokine staining and measurement of ex vivo cytolytic function. Importantly, the combination of these vaccine modalities also induced a sizeable expansion in most macaques of Gag-specific CD8-(CD4+) T-cells able to produce TNF-α. Hopefully, this modality of vaccine combination may be useful in the clinical management of HIV-1-infected individuals

  9. Deletion 6(p25.1) in a child with mild dysmorphic features and absence of major eye malformations: Implications for the location of genes involved in ocular development

    Energy Technology Data Exchange (ETDEWEB)

    Tepperberg, J.H.; Rao, K.W.; Albright, S.G. [Univ. of North Carolina, Chapel Hill, NC (United States)] [and others

    1994-09-01

    The authors describe a young girl with an apparently terminal deletion of chromosome 6 at p25.1 Fluorescence in situ hybridization with a chromosome 6 painting probe (ONCOR), showed that the abnormal chromosome 6 is composed entirely of chromosome 6 material. Analysis of the parents chromosomes with the same paint probe ruled out an inherited structural abnormality. To our knowledge, this case is the smallest terminal deletion of 6p yet reported. The patient is a 4 year, 6 month old female who was 3300g at birth. She has global developmental delay and little intelligible speech. Her weight is at the 25th centile, height at the 50th centile for a 3 1/2-year-old, and head circumference at the 25th centile. The eyes are prominent with shallow orbits. She has mild hyperopia and astigmatism. The philtrum is short and the vermillion border is thin. The midface is hypoplastic and there is dental malocclusion. Some of the common features reported in individuals with larger 6p terminal deletions include mental retardation, microcephaly, eye and ear abnormalities, short neck/excess nuchal skin, and flat broad nasal bridge. Our patient lacks several of the features reported in patients with larger deletions of 6p, including the more severe eye defects (e.g., anterior segment malformations including Peters and Rieger anomalies) described in individuals with 6p23 or 24 terminal deletions. This patient could be important in mapping the critical region for 6p deletion syndrome and for localizing clinical findings to a specific area of 6p. This case also raises the possibility that the gene(s) on 6p important for ocular growth and development are located proximal to 6p25.1.

  10. Synthesis and Crystal Structure of 4-(2-Bromophenyl)-3,4,7,8-tetrahydro-7,7-dimethyl-1-p-tolylquinoline-2,5(1H,6H)-dione

    Institute of Scientific and Technical Information of China (English)

    WANG Xiang-Shan; LI Qing; WU Jian-Rong; YAO Chang-Sheng

    2009-01-01

    The title compound 4-(2-bromophenyl)-3,4,7,8-tetrahydro-7,7-dimethyl-1-p-tolyl-quinoline-2,5(1H,6H)-dione 1 (C24H24BrNO2,Mr=438.35) was synthesized and characterized by IR,1H NMR and elemental analysis.The crystal belongs to monoclinic,space group C21c with a=27.565(14),b=10.079(5),c=15.917(8) (A),β=111.059(9)°,Z=8,V=4127(4)(A)3,Dc=1.411g·cm-3,μ(MoKα)=2.011 mm-1,F(000)=1808,the final R=0.0417 and wR=0.1032 for 2393 observed reflections (I > 2σ(I)).X-ray analysis reveals that the pyridine ring adopts a distorted boat conformation,while another six-membered ring takes a half-chair conformation.In addition,there are non-classical hydrogen bonds of C-H…O and C-H…Br in the structure.The short distance (3.481 (A))between the adjacent 2-bromophenyl rings indicates the existence of π-π interaction.

  11. Tracking past changes in lake-water phosphorus with a 251-lake calibration dataset in British Columbia: tool development and application in a multiproxy assessment of eutrophication and recovery in Osoyoos Lake, a transboundary lake in western North America

    Directory of Open Access Journals (Sweden)

    Brian Fraser Cumming

    2015-08-01

    Full Text Available Recently there has been an active discussion about the potential and challenges of tracking past lake-water trophic state using paleolimnological methods. Herein, we present analyses of the relationship between modern-day diatom assemblages from the surface sediments of 251 fresh-water lakes from British Columbia and contemporary limnological variables. Total phosphorus (TP was significantly related to the modern distribution of diatom assemblages. The large size of this new calibration dataset resulted in higher abundances and occurrences of many diatom taxa thereby allowing a more accurate quantification of the optima of diatom taxa to TP in comparison to previous smaller calibration datasets. Robust diatom-based TP inference models with a moderate predictive power were developed using weighted-averaging regression and calibration. Information from the calibration dataset was used to interpret changes in the diatom assemblages from the north and south basins of Osoyoos Lake, in conjunction with fossil pigment analyses. Osoyoos Lake is a large salmon-bearing lake that straddles the British Columbia-Washington border and has undergone cultural eutrophication followed by recovery due to substantial mitigation efforts in managing sources of nutrients. Both diatom assemblages and sedimentary pigments indicate that eutrophication began c. 1950 in the north basin and c. 1960 in the southern basin, reaching peak levels of production between 1960 and 1990, after which decreases in sedimentary pigments occurred, as well as decreases in the relative abundance and concentrations of diatom taxa inferred to have high TP optima. Post-1990 changes in the diatom assemblage suggests conditions have become less productive with a shift to taxa more indicative of lower TP optima in concert with measurements of declining TP, two of these diatom taxa, Cyclotella comensis and Cyclotella gordonensis, that were previously rare are now abundant.

  12. Anti-glioma Effect and Mechanism of Alkali Hydrolysate of Total Saponins from Pulsatilla chinensis on Human Glioma U251 Cells%白头翁总皂苷碱水解产物的抗胶质瘤作用及机制研究

    Institute of Scientific and Technical Information of China (English)

    彭翠平; 许琼明; 张天; 李笑然; 杨世林; 周英; 刘艳丽

    2014-01-01

    To investigate antitumor effect and mechanism of alkali hydrolysate of total saponins from Pulsatilla chinensis on human glioma U251 cells. Methods MTT assay and colony formation test were used to examine the growth of human glioma cells and colony formation rate. Meanwhile, Giemsa staining was used to observe the morphology of tumor cells, flow cytometry was used to detect the apoptosis of tumor cells, Hoechst 33342 assay kits were adopted to observe the morphology of apoptotic cells, and the expression levels of Caspase-3 and Bcl-2 protein were evaluated by Western blot assay. Results Alkali hydrolysate of total saponins from Pulsatilla chinensis exhibited an inhibition effect on the proliferation and colony formation of U251 cells in dose-dependent manner. The alkali hydrolysate at the dosage of 6.25, 9.375, 12.5 μg·mL-1 could induce apoptosis of U251 cells, and the apoptotic rates were(6.00±2.05)%, (46.19±0.24)% and(78.26±2.10)%, respectively. Western blotting results showed that the alkali hydrolysate could up-regulate the expression levels of Caspase-3 and Bcl-2. Conclusions Alkali hydrolysate of total saponins from Pulsatilla chinensis exerts satisfactory anti-glioma action, and the mechanism might be related with the regulation of Bcl-2 and Caspase-3 protein expression.%目的:探讨白头翁总皂苷碱水解产物(PAHS)的抗胶质瘤作用及机制。方法采用MTT法、集落形成实验分别检测细胞存活率和集落形成率,同时采用 Giemsa 染色观察 PAHS 对肿瘤细胞形态学变化;采用hoechest33342染色观察人脑胶质母细胞瘤细胞株U251凋亡细胞形态变化;用流式细胞仪检测细胞的凋亡;用Western Blot法检测Bcl-2和Caspase-3蛋白的表达变化。结果 PAHS可以剂量依赖性的抑制U251细胞的增殖,抑制U251细胞集落的形成; PAHS(6.25,9.375,12.5μg·mL-1)可以诱导U251细胞凋亡,抑制率分别为(6.00±2.05)%、(46.19±0.24)%、(78.26±2.10

  13. 36 CFR 251.57 - Rental fees.

    Science.gov (United States)

    2010-07-01

    ... enterprises, and which is engaged in public or semi-public activity to further public health, safety, or... authorized officer, when equitable and in the public interest, for the use and occupancy of National Forest... in the fees for an authorized use or occupancy for which the United States is already...

  14. 36 CFR 251.61 - Modifications.

    Science.gov (United States)

    2010-07-01

    ... plans, map(s), or survey(s) upon completion of construction. (c) A holder shall obtain prior approval... continued or revised, or a new authorization issued. (2) Changes during construction, or at any other...

  15. 30 CFR 206.251 - Definitions.

    Science.gov (United States)

    2010-07-01

    .... Lease means any contract, profit-share arrangement, joint venture, or other agreement issued or approved..., consortium, or joint venture. Sales type code means the contract type or general disposition (e.g., arm's... costs incurred by the lessee for coal washing. Transportation allowance means an allowance for...

  16. 40 CFR 60.251 - Definitions.

    Science.gov (United States)

    2010-07-01

    ... (incorporated by reference, see § 60.17). (b) Bag leak detection system means a system that is capable of... bag leaks and other upset conditions. A bag leak detection system includes, but is not limited to, an... limited to, breakers, crushers, screens, and conveyor belts. Equipment located at the mine face is...

  17. 37 CFR 251.3 - Arbitrator lists.

    Science.gov (United States)

    2010-07-01

    ... may consider relevant. (b) After January 1, 1998, and every two years thereafter, the Librarian of..., submitted to the Librarian from at least three professional arbitration associations or organizations. The.... With respect to persons on the arbitrator list, the Librarian will make available for copying...

  18. 37 CFR 251.21 - Public records.

    Science.gov (United States)

    2010-07-01

    ... records of the Librarian of Congress assembled and/or created under 17 U.S.C. 801 and 802, are available..., reports, and findings on any CARP matter or proceeding; (6) Communications among the Librarian of Congress...) Records not herein listed but which may be withheld as “exempted” if a CARP or the Librarian of...

  19. 30 CFR 251.1 - Definitions.

    Science.gov (United States)

    2010-07-01

    ..., geopressured-geothermal and associated resources, and all other minerals which are authorized by an Act of... National Register of Historic Places as defined in 36 CFR 60.4. Third Party means any person other than the... meaning: Act means the Outer Continental Shelf Lands Act (OCSLA), as amended (43 U.S.C. 1331 et...

  20. 36 CFR 251.35 - Petersburg watershed.

    Science.gov (United States)

    2010-07-01

    ... Forest timber (36 CFR part 223). In any removal of timber from the watershed, the Forest Supervisor shall... 36 CFR 261.1b. (e) The Forest Supervisor of the Stikine Area of the Tongass National Forest may... 36 Parks, Forests, and Public Property 2 2010-07-01 2010-07-01 false Petersburg watershed....

  1. 37 CFR 251.39 - Remedies.

    Science.gov (United States)

    2010-07-01

    ....39 Patents, Trademarks, and Copyrights COPYRIGHT OFFICE, LIBRARY OF CONGRESS COPYRIGHT ARBITRATION ROYALTY PANEL RULES AND PROCEDURES COPYRIGHT ARBITRATION ROYALTY PANEL RULES OF PROCEDURE Standards of... standards of conduct of this section may include, but are not limited to, the following— (a) In the case...

  2. 37 CFR 251.31 - Financial interests.

    Science.gov (United States)

    2010-07-01

    ... ARBITRATION ROYALTY PANEL RULES AND PROCEDURES COPYRIGHT ARBITRATION ROYALTY PANEL RULES OF PROCEDURE... indirect financial interest— (1) In the case of a distribution proceeding, in any claimant to the... from such claimants because of their representation; (2) In the case of a rate adjustment...

  3. 16 CFR 251.1 - The guide.

    Science.gov (United States)

    2010-01-01

    ... questions include “__ Cents-Off”, “Half-Price Sale”, “1/2 Off”, etc. See the Commission's “Fair Packaging... informs the customer of the location, elsewhere on the package or label, where the disclosures required...

  4. 37 CFR 251.1 - Official addresses.

    Science.gov (United States)

    2010-07-01

    ... ARBITRATION ROYALTY PANEL RULES AND PROCEDURES COPYRIGHT ARBITRATION ROYALTY PANEL RULES OF PROCEDURE... Copyright Arbitration Royalty Panels (CARPs) must be addressed as follows: (a) If hand delivered by a... Friday between 8:30 a.m. and 5 p.m. (b) If hand delivered by a commercial courier (excluding...

  5. 36 CFR 251.51 - Definitions.

    Science.gov (United States)

    2010-07-01

    ... CFR parts 1500-1508, 7 CFR part 1b, Forest Service Manual Chapter 1950, and Forest Service Handbook... equipment on National Forest System lands that involves the advertisement of a product or service, the... may require an environmental impact statement. Minor category—A processing or monitoring...

  6. 7 CFR 251.10 - Miscellaneous provisions.

    Science.gov (United States)

    2010-01-01

    ... financial records in accordance with 7 CFR part 3016, State agencies must maintain records to document the... warehousing practices, inventory controls, approval of distribution sites, reporting and...

  7. 37 CFR 251.13 - Closed meetings.

    Science.gov (United States)

    2010-07-01

    ... an arbitration, or a particular case of formal agency adjudication pursuant to 5 U.S.C. 554, or...; or (g) If there would be disclosure of investigatory records compiled for law enforcement, or... confidential source or, in the case of a criminal investigation or a national security...

  8. 30 CFR 251.2 - Purpose of this part.

    Science.gov (United States)

    2010-07-01

    ... Mineral Resources MINERALS MANAGEMENT SERVICE, DEPARTMENT OF THE INTERIOR OFFSHORE GEOLOGICAL AND... environmentally sound manner so as to prevent harm or damage to, or waste of, any natural resources (including any..., or the marine, coastal, or human environment. (c) To inform you and third parties of your legal...

  9. Dicty_cDB: SSA251 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available 60 4 AC116982 |AC116982.2 Dictyostelium discoideum chromosome 2 map 3622643-3879522 stra...yostelium discoideum strain QS... 284 2e-75 FJ374912_1( FJ374912 |pid:none) Dictyostelium discoideum stra...in QS... 284 2e-75 FJ374901_1( FJ374901 |pid:none) Dictyostelium discoideum strain QS...... 282 9e-75 FJ374891_1( FJ374891 |pid:none) Dictyostelium discoideum strain QS...... 282 9e-75 FJ374902_1( FJ374902 |pid:none) Dictyostelium discoideum strain QS... 282 9e-75 FJ374895_1( FJ374895 |pid:none) Dict

  10. 76 FR 251 - Country of Origin Labeling of Packed Honey

    Science.gov (United States)

    2011-01-04

    ... Service 7 CFR Part 52 RIN 0581-AC89 Country of Origin Labeling of Packed Honey AGENCY: Agricultural..., establishing new regulations addressing country of origin labeling for packed honey bearing any official USDA... service for honey if country of origin labeling requirements are not met for packages of honey...

  11. Dicty_cDB: SSF251 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available BH142335 |BH142335.1 TDGDN67TH cTOG Lycopersicon esculentum genomic clone cTOG23L14, DNA sequence. 36 0.041...nce. 42 0.045 2 AL844505 |AL844505.1 Plasmodium falciparum chromosome 6. 38 0.062 6 BH142885 |BH142885.1 TDGDT76TH cTOG Lycopersico...re E Sequences producing significant alignments: (bits) Value AE015450_422( AE015450 |pid:none) Myco...kkkkkkkk Homology vs CSM-cDNA Score E Sequences producing significant alignments: (bits) ...re E Sequences producing significant alignments: (bits) Value N AC117070 |AC117070.2 Dictyostelium discoideum chrom

  12. 40 CFR 264.251 - Design and operating requirements.

    Science.gov (United States)

    2010-07-01

    ... the pile contains any particulate matter which may be subject to wind dispersal, the owner or operator... collection and removal systems, is also a leak detection system. This leak detection system must be capable... life and post-closure care period. The requirements for a leak detection system in this paragraph...

  13. 24 CFR 983.251 - How participants are selected.

    Science.gov (United States)

    2010-04-01

    ...) The protections for victims of domestic violence, dating violence, and stalking in 25 CFR part 5.... (However, the PHA may deny assistance for the grounds specified in 24 CFR 982.552 and 982.553.) Admission of such families is not subject to income-targeting under 24 CFR 982.201(b)(2)(i), and such...

  14. 36 CFR 2.51 - Public assemblies, meetings.

    Science.gov (United States)

    2010-07-01

    ... facilities, impairment of a protected area's atmosphere of peace and tranquillity, interference with program... maintained in wilderness, natural, historic or commemorative zones; or (3) Unreasonably interfere with... protection and use of the park area for the purposes for which it is established. It may also...

  15. 48 CFR 952.251-70 - Contractor employee travel discounts.

    Science.gov (United States)

    2010-10-01

    ... eligible for GSA contract city pair fares. (c) Discount rail service. AMTRAK voluntarily offers discounts... employees. (d) Hotels/motels. Many lodging providers extend their discount rates for Federal employees to... NAME OF TRAVELER), the bearer of this letter is an employee of (COMPANY NAME) which has a contract...

  16. 24 CFR 251.2 - GNMA right to assignment.

    Science.gov (United States)

    2010-04-01

    ... right to assignment. If the lender-issuer defaults on its obligations under the GNMA Mortgage-Backed...-issuer, GNMA will have the right to perfect an assignment of the mortgage to itself. However, before exercising this right, GNMA will attempt to have the Mortgage assigned to another eligible coinsuring...

  17. 37 CFR 251.37 - Use of nonpublic information.

    Science.gov (United States)

    2010-07-01

    ... COPYRIGHT ARBITRATION ROYALTY PANEL RULES AND PROCEDURES COPYRIGHT ARBITRATION ROYALTY PANEL RULES OF... arbitration panel has ruled to be confidential in nature. (b) Unless required by law, no arbitrator shall... information, to further his or her private interest or that of another, whether through advice...

  18. 37 CFR 251.8 - Suspension of proceedings.

    Science.gov (United States)

    2010-07-01

    ... part, the Librarian will order a suspension of any ongoing hearing or other proceeding by notice in... Librarian will take the necessary steps to replace the arbitrator or arbitrators, and upon such replacement... emergency affecting an arbitrator, the Librarian considers a suspension of a proceeding necessary and...

  19. 36 CFR 251.95 - Authority of reviewing officer.

    Science.gov (United States)

    2010-07-01

    ... Reviewing Officer may issue one decision in cases involving separate reviews filed pursuant to 36 CFR part... 36 Parks, Forests, and Public Property 2 2010-07-01 2010-07-01 false Authority of reviewing....95 Authority of reviewing officer. (a) Discretion to establish procedures. A Reviewing Officer...

  20. 29 CFR 1926.251 - Rigging equipment for material handling.

    Science.gov (United States)

    2010-07-01

    ... employees. (4) Special custom design grabs, hooks, clamps, or other lifting accessories, for such units as... identification stating size, grade, rated capacity, and sling manufacturer. (2) Hooks, rings, oblong links, pear... chains, shall have a rated capacity at least equal to that of the chain. (3) Job or shop hooks and...

  1. 37 CFR 251.63 - Consideration of petition; settlements.

    Science.gov (United States)

    2010-07-01

    ... COPYRIGHT ARBITRATION ROYALTY PANEL RULES AND PROCEDURES COPYRIGHT ARBITRATION ROYALTY PANEL RULES OF... the case of a settlement among the parties to a proceeding, the Librarian may, upon the request of the... adopt the rate embodied in the proposed settlement without convening an arbitration panel, provided...

  2. 37 CFR 251.33 - Ex parte communications.

    Science.gov (United States)

    2010-07-01

    ... ARBITRATION ROYALTY PANEL RULES AND PROCEDURES COPYRIGHT ARBITRATION ROYALTY PANEL RULES OF PROCEDURE... report to the Librarian, and, in the case of a remand, from the time of their reconvening to the time...

  3. 37 CFR 251.38 - Billing and commitment to standards.

    Science.gov (United States)

    2010-07-01

    ... COPYRIGHT ARBITRATION ROYALTY PANEL RULES AND PROCEDURES COPYRIGHT ARBITRATION ROYALTY PANEL RULES OF... case of an arbitrator who resides outside the Washington, DC metropolitan area, for travel,...

  4. 8 CFR 251.1 - Arrival manifests and lists.

    Science.gov (United States)

    2010-01-01

    ... immigration inspection is performed a manifest of all crewmen on board on Form I-418, Passenger List and Crew List, in accordance with the instructions contained thereon. (2) Longshore work notations. The master... of the owner, and that under 46 CFR 148.02 must be kept in a conspicuous place near the bridge...

  5. 7 CFR 764.251 - Operating loan uses.

    Science.gov (United States)

    2010-01-01

    ... reorganizing a farm to improve its profitability; (2) Purchase of livestock, including poultry, farm equipment... improve the farm's profitability provided the applicant has refinanced direct or guaranteed OL loans...

  6. 24 CFR 220.251 - Cross-reference.

    Science.gov (United States)

    2010-04-01

    ... Insurance Fund. 203.421Allocation of Mutual Mortgage Insurance Fund income or loss. 203.422Right and liability under Mutual Mortgage Insurance Fund. 203.423Distribution of distributive shares 203.424Maximum... construed to refer to section 220 of the act, and all references to the Mutual Mortgage Insurance Fund...

  7. 36 CFR 251.83 - Decisions not appealable.

    Science.gov (United States)

    2010-07-01

    ... appealable to the Agriculture Board of Contract Appeals, USDA, under 7 CFR part 24. (b) Decisions involving Freedom of Information Act denials under 7 CFR part 1 or Privacy Act determinations under 7 CFR 1.118. (c... USES Appeal of Decisions Relating to Occupancy and Use of National Forest System Lands §...

  8. 7 CFR 25.1 - Applicability and scope.

    Science.gov (United States)

    2010-01-01

    ... Office of the Secretary of Agriculture RURAL EMPOWERMENT ZONES AND ENTERPRISE COMMUNITIES General... applicable to rural empowerment zones and enterprise communities, authorized under the Omnibus Budget... area requirements, the nomination process for rural Empowerment Zones and rural Enterprise...

  9. Time Warp Operating System, Version 2.5.1

    Science.gov (United States)

    Bellenot, Steven F.; Gieselman, John S.; Hawley, Lawrence R.; Peterson, Judy; Presley, Matthew T.; Reiher, Peter L.; Springer, Paul L.; Tupman, John R.; Wedel, John J., Jr.; Wieland, Frederick P.; Younger, Herbert C.

    1993-01-01

    Time Warp Operating System, TWOS, is special purpose computer program designed to support parallel simulation of discrete events. Complete implementation of Time Warp software mechanism, which implements distributed protocol for virtual synchronization based on rollback of processes and annihilation of messages. Supports simulations and other computations in which both virtual time and dynamic load balancing used. Program utilizes underlying resources of operating system. Written in C programming language.

  10. 36 CFR 251.85 - Election of appeal process.

    Science.gov (United States)

    2010-07-01

    ... under the provisions of 36 CFR part 217 may intervene pursuant to 36 CFR 217.6(b). ... 36 Parks, Forests, and Public Property 2 2010-07-01 2010-07-01 false Election of appeal process... Election of appeal process. (a) No decision can be appealed by the same person under both this subpart...

  11. Use of trioctylphosphine oxide for transplutonium element extraction and purification

    International Nuclear Information System (INIS)

    Investigated was extraction of tri-valent curium, berkelium, californium, einsteinium as well as cerium and europium with trioctylphosphin oxide from lactic acid solutions, containing DTPA and aluminium nitrate depending on the aluminium nitrate and TOPO concentrations and nitric acid solutions of variable concentration as well. Under optimum conditions of extraction chromatography of berkelium studied was the distribution of cobalt, nickel, chromium, iron, aluminium, titanium, zirconium and niobium ions, and the coefficients of berkelium purification from cations investigated were determined. The effect of weight quantities of cation impurities on extraction chromatographic yield of berkelium has been investigated. Examples of practice application of the extraction chromatography with the use of TOPO are given

  12. Actinide production in the reaction of heavy ions with curium-248

    International Nuclear Information System (INIS)

    Chemical experiments were performed to examine the usefulness of heavy ion transfer reactions in producing new, neutron-rich actinide nuclides. A general quasi-elastic to deep-inelastic mechanism is proposed, and the utility of this method as opposed to other methods (e.g. complete fusion) is discussed. The relative merits of various techniques of actinide target synthesis are discussed. A description is given of a target system designed to remove the large amounts of heat generated by the passage of a heavy ion beam through matter, thereby maximizing the beam intensity which can be safely used in an experiment. Also described is a general separation scheme for the actinide elements from protactinium (Z=91) to mendelevium (Z=101), and fast specific procedures for plutonium, americium and berkelium. The cross sections for the production of several nuclides from the bombardment of 248Cm with 18O, 86Kr and 136Xe projectiles at several energies near and below the Coulomb barrier were determined. The results are compared with yields from 48Ca and 238U bombardments of 248Cm. Simple extrapolation of the product yields into unknown regions of charge and mass indicates that the use of heavy ion transfer reactions to produce new, neutron-rich above-target species is limited. The substantial production of neutron-rich below-target species, however, indicates that with very heavy ions like 136Xe and 238U the new species 248Am, 249Am and 247Pu should be produced with large cross sections from a 248Cm target. A preliminary, unsuccessful attempt to isolate 247Pu is outlined. The failure is probably due to the half life of the decay, which is calculated to be less than 3 minutes. The absolute gamma ray intensities from 251Bk decay, necessary for calculating the 251Bk cross section, are also determined

  13. ФАКУЛЬТАТИВНЫЕ ПРИЗНАКИ ОБЪЕКТИВНОЙ СТОРОНЫ ПРЕСТУПЛЕНИЯ, ПРЕДУСМОТРЕННОГО СТ. 251 УГОЛОВНОГО КОДЕКСАРОССИЙСКОЙ ФЕДЕРАЦИИ «ЗАГРЯЗНЕНИЕ АТМОСФЕРЫ»

    OpenAIRE

    БОКУЦ ЕЛЕНА ЮРЬЕВНА

    2015-01-01

    Автор статьи рассматривает вопросы квалификации загрязнения атмосферы, предусмотренного ст.251 Уголовного кодекса Российской Федерации, по факультативным признакам объективной стороны состава преступленияThe author of the article considers the issues of qualification of air pollution under Art. 251 of the Criminal code of the Russian Federation, under the additional characteristics of the objective side of crime....

  14. Dynamics of viral replication in blood and lymphoid tissues during SIVmac251 infection of macaques

    Directory of Open Access Journals (Sweden)

    Mannioui Abdelkrim

    2009-01-01

    Full Text Available Abstract Background Extensive studies of primary infection are crucial to our understanding of the course of HIV disease. In SIV-infected macaques, a model closely mimicking HIV pathogenesis, we used a combination of three markers -- viral RNA, 2LTR circles and viral DNA -- to evaluate viral replication and dissemination simultaneously in blood, secondary lymphoid tissues, and the gut during primary and chronic infections. Subsequent viral compartmentalization in the main target cells of the virus in peripheral blood during the chronic phase of infection was evaluated by cell sorting and viral quantification with the three markers studied. Results The evolutions of viral RNA, 2LTR circles and DNA levels were correlated in a given tissue during primary and early chronic infection. The decrease in plasma viral load principally reflects a large decrease in viral replication in gut-associated lymphoid tissue (GALT, with viral RNA and DNA levels remaining stable in the spleen and peripheral lymph nodes. Later, during chronic infection, a progressive depletion of central memory CD4+ T cells from the peripheral blood was observed, accompanied by high levels of viral replication in the cells of this subtype. The virus was also found to replicate at this point in the infection in naive CD4+ T cells. Viral RNA was frequently detected in monocytes, but no SIV replication appeared to occur in these cells, as no viral DNA or 2LTR circles were detected. Conclusion We demonstrated the persistence of viral replication and dissemination, mostly in secondary lymphoid tissues, during primary and early chronic infection. During chronic infection, the central memory CD4+ T cells were the major site of viral replication in peripheral blood, but viral replication also occurred in naive CD4+ T cells. The role of monocytes seemed to be limited to carrying the virus as a cargo because there was an observed lack of replication in these cells. These data may have important implications for the targeting of HIV treatment to these diverse compartments.

  15. 49 CFR 40.251 - What are the first steps in an alcohol confirmation test?

    Science.gov (United States)

    2010-10-01

    ... period (i.e., to prevent an accumulation of mouth alcohol from leading to an artificially high reading... 49 Transportation 1 2010-10-01 2010-10-01 false What are the first steps in an alcohol... FOR TRANSPORTATION WORKPLACE DRUG AND ALCOHOL TESTING PROGRAMS Alcohol Confirmation Tests §...

  16. 7 CFR 1726.251 - Prior approved contract modification related to price escalation on transmission equipment...

    Science.gov (United States)

    2010-01-01

    ... engineer, if applicable) will select the indexes for the particular item to be used in the price adjustment... Petroleum Rate (057) from the month in which the bids are opened to the month in which the oil is...

  17. 36 CFR 251.124 - Preferred operator competitive special use authorization procedures.

    Science.gov (United States)

    2010-07-01

    ... entrepreneurship, nonprofit entity, or other form of organization shall be considered valid only when the... entrepreneurship, nonprofit entity, or other form of organization. (f) A qualified preferred operator shall...

  18. THE CANNABINOID RECEPTOR ANTAGONIST AM251 INCREASES PARAOXON AND CHLORPYRIFOS OXON TOXICITY IN RATS

    OpenAIRE

    Liu, Jing; Pope, Carey

    2014-01-01

    Organophosphorus anticholinesterases (OPs) elicit acute toxicity by inhibiting acetylcholinesterase (AChE), leading to acetylcholine accumulation and overstimulation of cholinergic receptors. Endocannabinoids (eCBs, e.g., arachidonoyl ethanolamide [AEA] and 2-arachidonoyl glycerol [2-AG]) are neuromodulators that regulate neurotransmission by reducing neurotransmitter release. The eCBs are degraded by the enzymes fatty acid amide hydrolase (FAAH, primarily involved in hydrolysis of AEA) and m...

  19. 37 CFR 251.34 - Gifts and other things of monetary value.

    Science.gov (United States)

    2010-07-01

    ... under circumstances in which it is clear that the gift is motivated by a family relationship or personal... relationship to the arbitrator, or (2) Given to any other person, including any charitable organization, on...

  20. (E-1-Phenyl-2-({5-[(1E-(2-phenylhydrazin-1-ylidenemethyl]-2-thienyl}methylidenehydrazine

    Directory of Open Access Journals (Sweden)

    James L. Wardell

    2010-03-01

    Full Text Available The title molecule, C18H16N4S, adopts a U-shape with the aromatic groups lying syn and orientated in the same direction as the thiophene S atom. The conformation about each of the C=N bonds is E. Overall, the molecule is curved as seen in the dihedral angle of 30.26 (19° formed between the terminal benzene rings. In the crystal, supramolecular chains along the c axis are formed by a combination of N—H...N hydrogen bonds and N—H...π interactions.

  1. People and things. CERN Courier, Jan-Feb 1985, v. 25(1)

    International Nuclear Information System (INIS)

    The article reports on achievements of various people, staff changes and position opportunities within the CERN organization and contains news updates on upcoming or past events. The General Assembly of the International Union of Pure and Applied Physics (IUPAP) met in Trieste in October. At this meeting the People 's Republic of China became a full member of IUPAP. Last year, voting took place for the new Executive Committee of the Division of Particles and Fields of the American Physical Society. A NATO Advanced Study Institute 'New Vistas in Electro-Nuclear Physics' will be held in Banff, Alberta, Canada, from 22 August to 4 September. The 1985 International Symposium on Lepton and Photon Interactions at High Energies is being held in Kyoto from 19-24 August. A conference on Computing in High Energy Physics will be held at Amsterdam, the Netherlands University of Amsterdam and will be devoted mainly to three subjects: networking, embedded systems and vector and parallel processing. The 1985 CERN/Joint Institute for Nuclear Research (JINR, Dubna, USSR) School of Physics, the ninth in the series, will be held from 1-14 September at Urbino, Italy. The basic aim of these schools is to teach various aspects of high energy physics, especially theoretical, to young experimentalists drawn mainly from CERN and JINR Member States

  2. 48 CFR 52.251-2 - Interagency Fleet Management System Vehicles and Related Services.

    Science.gov (United States)

    2010-10-01

    ... vehicles and the use of related services by the Contractor shall be in accordance with 41 CFR 101-39 and 41 CFR 101-38.301-1. (End of clause) ... 48 Federal Acquisition Regulations System 2 2010-10-01 2010-10-01 false Interagency...

  3. Decree no. 2004-251 from March 19, 2004 relative to public utility obligations in the gas sector; Decret no. 2004-251 du 19 mars 2004 relatif aux obligations de service public dans le secteur du gaz

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2004-03-01

    This decree defines the public utility obligations that gas suppliers and gas facility operators (transportation and distribution networks, underground storage facilities, LNG facilities) have to fulfill in order to ensure without interruption the continuity of gas supplies to their clients. (J.S.)

  4. A new radiation infrastructure for the Modular Earth Submodel System (MESSy, based on version 2.51)

    Science.gov (United States)

    Dietmüller, Simone; Jöckel, Patrick; Tost, Holger; Kunze, Markus; Gellhorn, Catrin; Brinkop, Sabine; Frömming, Christine; Ponater, Michael; Steil, Benedikt; Lauer, Axel; Hendricks, Johannes

    2016-06-01

    The Modular Earth Submodel System (MESSy) provides an interface to couple submodels to a base model via a highly flexible data management facility (Jöckel et al., 2010). In the present paper we present the four new radiation related submodels RAD, AEROPT, CLOUDOPT, and ORBIT. The submodel RAD (including the shortwave radiation scheme RAD_FUBRAD) simulates the radiative transfer, the submodel AEROPT calculates the aerosol optical properties, the submodel CLOUDOPT calculates the cloud optical properties, and the submodel ORBIT is responsible for Earth orbit calculations. These submodels are coupled via the standard MESSy infrastructure and are largely based on the original radiation scheme of the general circulation model ECHAM5, however, expanded with additional features. These features comprise, among others, user-friendly and flexibly controllable (by namelists) online radiative forcing calculations by multiple diagnostic calls of the radiation routines. With this, it is now possible to calculate radiative forcing (instantaneous as well as stratosphere adjusted) of various greenhouse gases simultaneously in only one simulation, as well as the radiative forcing of cloud perturbations. Examples of online radiative forcing calculations in the ECHAM/MESSy Atmospheric Chemistry (EMAC) model are presented.

  5. Immunization of Newborn Rhesus Macaques with Simian Immunodeficiency Virus (SIV) Vaccines Prolongs Survival after Oral Challenge with Virulent SIVmac251

    OpenAIRE

    Van Rompay, Koen K. A.; Greenier, Jennifer L.; Cole, Kelly Stefano; Earl, Patricia; Moss, Bernard; Steckbeck, Jonathan D.; Pahar, Bapi; Rourke, Tracy; Montelaro, Ronald C.; Canfield, Don R; Tarara, Ross P; Miller, Christopher; McChesney, Michael B.; Marthas, Marta L.

    2003-01-01

    There is an urgent need for active immunization strategies that, if administered shortly after birth, could protect infants in developing countries from acquiring human immunodeficiency virus (HIV) infection through breast-feeding. Better knowledge of the immunogenic properties of vaccine candidates in infants and of the effect of maternal antibodies on vaccine efficacy will aid in the development of such a neonatal HIV vaccine. Simian immunodeficiency virus (SIV) infection of infant macaques...

  6. Mutp53 non-B DNA structure binding to intronic sequences modulates gene expression in U251 cells

    Czech Academy of Sciences Publication Activity Database

    Tichý, Vlastimil; Brázdová, Marie; Quante, T.; Togel, L.; Walter, K.; Loscher, C.; Navrátilová, Lucie; Lexa, M.; Deppert, W.; Tolstonog, G.V.

    Prague, 2009. s. 13. [Young Scientist Forum - FEBS: Labyrinth of cells and molecules. 02.07.2009-04.07.2009, Prague] R&D Projects: GA MŠk(CZ) 1K04119; GA MŠk(CZ) LC06035; GA ČR(CZ) GP204/06/P369; GA ČR(CZ) GA204/08/1560; GA AV ČR(CZ) IAA500040701 Institutional research plan: CEZ:AV0Z50040507; CEZ:AV0Z50040702 Keywords : mutant p53 * gene regulation * glioblastoma cells Subject RIV: BO - Biophysics

  7. Mutp53 non-B DNA structure binding to intronic sequences modulates gene expression in U251 cells

    Czech Academy of Sciences Publication Activity Database

    Tichý, Vlastimil; Brázdová, Marie; Quante, T.; Togel, L.; Walter, K.; Loscher, C.; Navrátilová, Lucie; Lexa, M.; Deppert, W.; Tolstonog, G.V.

    Prague, 2009. s. 393. ISSN 1742-464X. [34th FEBS Congress: Life's Molecular Interactions. 04.07.2009-09.07.2009, Prague] R&D Projects: GA MŠk(CZ) 1K04119; GA MŠk(CZ) LC06035; GA ČR(CZ) GA204/08/1560; GA ČR(CZ) GP204/06/P369; GA AV ČR(CZ) IAA500040701 Institutional research plan: CEZ:AV0Z50040507; CEZ:AV0Z50040702 Keywords : mutant p53 * gene regulation * glioblastoma cells Subject RIV: BO - Biophysics

  8. 76 FR 16038 - Proposed Collection; Comment Request for Prepaid Card Marketing Customer Survey, CS-10-251.

    Science.gov (United States)

    2011-03-22

    ..., IRS is hoping to leverage the theory and principles of social marketing. Social marketing principles and practices apply marketing principles to social programs. This data will provide the IRS with... Internal Revenue Service Proposed Collection; Comment Request for Prepaid Card Marketing Customer...

  9. (E-1-(3-Nitrophenyl-2-({5-[(1E-2-(3-nitrophenylhydrazin-1-ylidenemethyl]-2-thienyl}methylidenehydrazine

    Directory of Open Access Journals (Sweden)

    Geraldo M. de Lima

    2010-02-01

    Full Text Available The title molecule, C18H14N6O4S, adopts a U-shape with the aromatic groups lying syn and oriented in the same direction as the thiophene S atom. Twists away from planarity are evident with the maximum deviation being found for a terminal nitro group: C/C/N/O = 19.0 (3°. The conformation about each of the C=N bonds is E. In the crystal, centrosymmetrically related molecules are connected via N—H...Onitro hydrogen bonds, forming 14-membered {...HNC3NO}2 synthons. These are linked into layers via C—H...Onitro interactions with the primary interactions between layers being of the type C—H...π, where the π-system is the thiophene ring.

  10. 46 CFR 251.1 - Applications for construction-differential subsidy under Title V, Merchant Marine Act, 1936, as...

    Science.gov (United States)

    2010-10-01

    ... capability and productivity possible. In making its determinations under this policy, the Maritime Subsidy... cost of construction. (5) Estimated revenues and cost of operation; and with respect to wage cost, the... such a fashion as will measure the productivity of the vessel (i.e., its carrying capacity, speed,...

  11. Discovery of Isotopes of the Transuranium Elements with 93 <= Z <= 98

    OpenAIRE

    Fry, C; Thoennessen, M

    2012-01-01

    One hundred and five isotopes of the transuranium elements neptunium, plutonium, americium, curium, berkelium and californium have so far been observed; the discovery of these isotopes is discussed. For each isotope a brief summary of the first refereed publication, including the production and identification method, is presented.

  12. http://periodicos.ufsc.br/index.php/gual/article/view/1983-4535.2012v5n2p251/22582

    Directory of Open Access Journals (Sweden)

    Alexandre Costa Quintana

    2012-08-01

    Full Text Available The process of virtual learning passes for the necessity of the existence of an evaluation process. Normally, this evaluation is integrated by a virtual part and another class attendance. The posting of the task becomes an important communication tool between students, teachers and tutors, as it enables the exchange of information between those involved, besides serving as a component of assessment and therefore reflecting the development of teaching-learning process.In this direction the objective of this work is in the distance to analyze the form of execution of the virtual tasks of the pupil of the course of graduation in Administration at distance. This work was elaborated, by means of a descriptive research, with analysis of content and a qualitative boarding. Concluding that the results of this study show that teachers, tutors and coordination of distance courses have attention on the needs of students in relation to time for implementation, appropriate date for posting and service to academics, that in understanding these will be key to the tasks are actually an important tool in the teaching-learning.

  13. Common variation at 2p13.3, 3q29, 7p13 and 17q25.1 associated with susceptibility to pancreatic cancer

    Czech Academy of Sciences Publication Activity Database

    Childs, E.J.; Mocci, E.; Campa, D.; Bracci, P. M.; Gallinger, S.; Goggins, M.; Li, D.; Neale, R.E.; Olson, S. H.; Scelo, G.; Amundadottir, L. T.; Bamlet, W.R.; Bijlsma, M.F.; Blackford, A.; Borges, M.; Brennan, P.; Brenner, H.; Bueno-de-Mesquita, H. B.; Canzian, F.; Capurso, G.; Cavestro, G.M.; Chaffee, K.G.; Chanock, S. J.; Cleary, S.P.; Cotterchio, M.; Foretová, L.; Fuchs, Ch.; Funel, N.; Gazouli, M.; Hassan, M.; Herman, J.M.; Holcatová, I.; Holly, E. A.; Hoover, R.N.; Hung, R.J.; Janout, V.; Key, T.J.; Kupcinskas, J.; Kurtz, R. C.; Landi, S.; Lu, S.; Malecka-Panas, E.; Mambrini, A.; Mohelníková-Duchoňová, B.; Neoptolemos, J.P.; Oberg, A. L.; Orlow, I.; Pasquali, C.; Pezzilli, R.; Rizzato, C.; Saldia, A.; Scarpa, A.; Stolzenberg-Solomon, R. S.; Strobel, O.; Tavano, F.; Vashist, Y.K.; Vodička, Pavel; Wolpin, B. M.; Yu, H.; Petersen, G.; Risch, H. A.; Klein, A. P.

    2015-01-01

    Roč. 47, č. 8 (2015), s. 911-918. ISSN 1061-4036 R&D Projects: GA MZd NR9422; GA ČR GAP301/12/1734 Institutional support: RVO:68378041 Keywords : genome-wide association * genotype imputation * lung adenocarcinoma * cigarette-smoking * genetic-variation * alpha-gene * risk * loci * population * mutations Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 29.352, year: 2014

  14. « The Petrography of Iranian Safavid Ceramics ». Journal of Archaeological Science, 30 (2003), pp. 251-261.

    OpenAIRE

    Miroudot, Delphine

    2005-01-01

    L’analyse pétrographique (par observation de lames minces au microscope polarisant) de céramiques iraniennes d’époque safavide a permis de caractériser cinq groupes que les auteurs tentent d’attribuer aux grands centres de production documentés par les sources historiques (tout particulièrement Mashhad, Isfahan, Kirman). En conclusion, ils confrontent leurs résultats aux attributions proposées voici quelques décennies par Arthur Lane selon des critères d’ordre stylistique ; elles se confirmen...

  15. 75 FR 43818 - Amendment of VOR Federal Airways V-50, V-251, and V-313 in the Vicinity of Decatur, IL

    Science.gov (United States)

    2010-07-27

    ... Regulatory Policies and Procedures (44 FR 11034; February 26, 1979); and (3) does not warrant preparation of.... 10854, 24 FR 9565, 3 CFR, 1959-1963 Comp., p. 389. Sec. 71.1 0 2. The incorporation by reference in 14... TRANSPORTATION Federal Aviation Administration 14 CFR Part 71 RIN: 2120-AA66 Amendment of VOR Federal Airways...

  16. Effect of CD8+ Lymphocyte Depletion on Virus Containment after Simian Immunodeficiency Virus SIVmac251 Challenge of Live Attenuated SIVmac239Δ3-Vaccinated Rhesus Macaques

    OpenAIRE

    Schmitz, Jörn E.; Johnson, R. Paul; McClure, Harold M.; Manson, Kelledy H.; Wyand, Michael S.; Kuroda, Marcelo J.; Lifton, Michelle A.; Khunkhun, Rajinder S.; McEvers, Kimberly J.; Gillis, Jacqueline; Piatak, Michael; Lifson, Jeffrey D.; Grosschupff, Gudrun; Racz, Paul; Tenner-Racz, Klara

    2005-01-01

    Although live attenuated vaccines can provide potent protection against simian immunodeficiency virus (SIV) and simian-human immunodeficiency virus challenges, the specific immune responses that confer this protection have not been determined. To test whether cellular immune responses mediated by CD8+ lymphocytes contribute to this vaccine-induced protection, we depleted rhesus macaques vaccinated with the live attenuated virus SIVmac239Δ3 of CD8+ lymphocytes and then challenged them with SIV...

  17. Binding of mutant p53 protein to non-B DNA structures in intronic and intergenic sequences modulates gene expression in U251 glioblastoma cells

    Czech Academy of Sciences Publication Activity Database

    Tichý, Vlastimil; Navrátilová, Lucie; Quante, T.; Tögel, L.; Walter, K.; Lexa, M.; Tolstonog, G.V.; Deppert, W.; Paleček, Emil; Fojta, Miroslav; Brázdová, Marie

    Brno, 2009. s. 43-44. ISBN 978-80-210-4830-0. [Pracovní setkání biochemiků a molekulárních biologů /13./. 14.04.2009-15.04.2009, Brno] R&D Projects: GA MŠk(CZ) 1K04119; GA MŠk(CZ) LC06035; GA ČR(CZ) GP204/06/P369; GA ČR(CZ) GA204/08/1560; GA AV ČR(CZ) IAA500040701 Institutional research plan: CEZ:AV0Z50040507; CEZ:AV0Z50040702 Keywords : mutant p53 * gene regulation * glioblastoma cells Subject RIV: BO - Biophysics

  18. Binding of mutant p53 protein to non-B DNA structures in intronic and intergenic sequences modulates gene expression in U251 glioblastoma cells

    Czech Academy of Sciences Publication Activity Database

    Tichý, Vlastimil; Navrátilová, Lucie; Quante, T.; Togel, L.; Walter, K.; Lexa, M.; Tolstonog, G.V.; Deppert, W.; Paleček, Emil; Fojta, Miroslav; Brázdová, Marie

    Brno, 2009. s. 39-40. [2nd joint meeting on the role of p53 , MDM2, AGR2/3 and ubiquitin/chaperone system in tumour biology. 27.04.2009-29.04.2009, Brno] R&D Projects: GA ČR(CZ) GP204/06/P369; GA ČR(CZ) GA204/08/1560; GA MŠk(CZ) 1K04119; GA MŠk(CZ) LC06035; GA AV ČR(CZ) IAA500040701 Institutional research plan: CEZ:AV0Z50040507; CEZ:AV0Z50040702 Keywords : mutant p53 * gene regulation * glioblastoma cells Subject RIV: BO - Biophysics

  19. Modulation of gene expression in U251 glioblastoma cells by binding of mutant p53 R273H to intronic and intergenic sequences

    Czech Academy of Sciences Publication Activity Database

    Brázdová, Marie; Quante, T.; Tögel, L.; Walter, K.; Loscher, Ch.; Tichý, Vlastimil; Činčárová, Lenka; Deppert, W.; Tolstonog, G.V.

    2009-01-01

    Roč. 37, č. 5 (2009), s. 1486-1500. ISSN 0305-1048 R&D Projects: GA ČR(CZ) GP204/06/P369; GA ČR(CZ) GA204/08/1560; GA MŠk(CZ) 1K04119 Institutional research plan: CEZ:AV0Z50040507; CEZ:AV0Z50040702 Keywords : mutant p53 * cancer * gene expression Subject RIV: BO - Biophysics Impact factor: 7.479, year: 2009

  20. Supplementary Info. to T. Elliot & P.L. Younger, Detection of mixing dynamics during pumping of a flooded coal mine. Groundwater, 52(2): 251–263

    OpenAIRE

    Elliot, Trevor; Younger, P. L.

    2013-01-01

    Contains 9 pages of text covering the History of the Deerplay workings, Test pumping of the Abstraction Borehole on Deerplay Moor, Methodology, Mineral Saturation Indices, Sulfur isotope systematics. Tables S1 and S2. Figures S1 to S5.

  1. Complete Genome Sequence of a Reference Stock of Simian Immunodeficiency Virus RNA (SIVmac251/32H/L28) Determined by Deep Sequencing.

    Science.gov (United States)

    Jenkins, Adrian; Ham, Claire; Almond, Neil; Berry, Neil

    2016-01-01

    A reference preparation for simian immunodeficiency virus (SIV) RNA nucleic acid assays was characterized by complete genome deep sequencing. The entire coding sequence and flanking long terminal repeats, including minority species, were determined. This information will inform SIV research investigations and aid evaluation and development of amplification assays for SIV RNA quantification. PMID:27231355

  2. Common alleles at 6q25.1 and 1p11.2 are associated with breast cancer risk for BRCA1 and BRCA2 mutation carriers

    DEFF Research Database (Denmark)

    Antoniou, Antonis C; Kartsonaki, Christiana; Sinilnikova, Olga M;

    2011-01-01

    independently associated with the risk of breast cancer for BRCA1 mutation carriers [hazard ratio (HR) = 1.17, 95% confidence interval (CI): 1.11-1.23, P-trend = 4.5 × 10(-9) for rs2046210; HR = 1.28, 95% CI: 1.18-1.40, P-trend = 1.3 × 10(-8) for rs9397435], but only rs9397435 was associated with the risk...... to a better understanding of the biology of tumour development in these women....

  3. RESGPSLNS_JD251.SHP: Ship Trackline Along Which Continuous Resistivity Profile (CRP) Data was Collected in the Potomac River/Chesapeake Bay on Sept. 8, 2006

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — In order to test hypotheses about groundwater flow under and into Chesapeake Bay, geophysical surveys were conducted by U.S. Geological Survey (USGS) scientists on...

  4. SU-E-J-251: Incorporation of Pre-Therapy 18F-FDG Uptake with CT Texture Features in a Predictive Model for Radiation Pneumonitis Development

    International Nuclear Information System (INIS)

    Purpose: To determine whether the addition of standardized uptake value (SUV) statistical variables to CT lung texture features can improve a predictive model of radiation pneumonitis (RP) development in patients undergoing radiation therapy. Methods: Anonymized data from 96 esophageal cancer patients (18 RP-positive cases of Grade ≥ 2) were retrospectively collected including pre-therapy PET/CT scans, pre-/posttherapy diagnostic CT scans and RP status. Twenty texture features (firstorder, fractal, Laws’ filter and gray-level co-occurrence matrix) were calculated from diagnostic CT scans and compared in anatomically matched regions of the lung. The mean, maximum, standard deviation, and 50th–95th percentiles of the SUV values for all lung voxels in the corresponding PET scans were acquired. For each texture feature, a logistic regression-based classifier consisting of (1) the average change in that texture feature value between the pre- and post-therapy CT scans and (2) the pre-therapy SUV standard deviation (SUVSD) was created. The RP-classification performance of each logistic regression model was compared to the performance of its texture feature alone by computing areas under the receiver operating characteristic curves (AUCs). T-tests were performed to determine whether the mean AUC across texture features changed significantly when SUVSD was added to the classifier. Results: The AUC for single-texturefeature classifiers ranged from 0.58–0.81 in high-dose (≥ 30 Gy) regions of the lungs and from 0.53–0.71 in low-dose (< 10 Gy) regions. Adding SUVSD in a logistic regression model using a 50/50 data partition for training and testing significantly increased the mean AUC by 0.08, 0.06 and 0.04 in the low-, medium- and high-dose regions, respectively. Conclusion: Addition of SUVSD from a pre-therapy PET scan to a single CT-based texture feature improves RP-classification performance on average. These findings demonstrate the potential for more accurate prediction of RP using information from multiple imaging modalities. Supported, in part, by the National Institute of Biomedical Imaging and Bioengineering of the National Institutes of Health under grant number T32 EB002103; SGA receives royalties and licensing fees through the University of Chicago for computer-aided diagnosis technology. HA receives royalties through the University of Chicago for computer-aided diagnosis technology

  5. SU-E-J-251: Incorporation of Pre-Therapy 18F-FDG Uptake with CT Texture Features in a Predictive Model for Radiation Pneumonitis Development

    Energy Technology Data Exchange (ETDEWEB)

    Anthony, G; Cunliffe, A; Armato, S; Al-Hallaq, H [The University of Chicago, Chicago, IL (United States); Castillo, R [Univ Texas Medical Branch of Galveston, Pearland, TX (United States); Pham, N [Baylor College of Medicine, Houston, TX (United States); Guerrero, T [Beaumont Health System, Royal Oak, MI (United States)

    2015-06-15

    Purpose: To determine whether the addition of standardized uptake value (SUV) statistical variables to CT lung texture features can improve a predictive model of radiation pneumonitis (RP) development in patients undergoing radiation therapy. Methods: Anonymized data from 96 esophageal cancer patients (18 RP-positive cases of Grade ≥ 2) were retrospectively collected including pre-therapy PET/CT scans, pre-/posttherapy diagnostic CT scans and RP status. Twenty texture features (firstorder, fractal, Laws’ filter and gray-level co-occurrence matrix) were calculated from diagnostic CT scans and compared in anatomically matched regions of the lung. The mean, maximum, standard deviation, and 50th–95th percentiles of the SUV values for all lung voxels in the corresponding PET scans were acquired. For each texture feature, a logistic regression-based classifier consisting of (1) the average change in that texture feature value between the pre- and post-therapy CT scans and (2) the pre-therapy SUV standard deviation (SUV{sub SD}) was created. The RP-classification performance of each logistic regression model was compared to the performance of its texture feature alone by computing areas under the receiver operating characteristic curves (AUCs). T-tests were performed to determine whether the mean AUC across texture features changed significantly when SUV{sub SD} was added to the classifier. Results: The AUC for single-texturefeature classifiers ranged from 0.58–0.81 in high-dose (≥ 30 Gy) regions of the lungs and from 0.53–0.71 in low-dose (< 10 Gy) regions. Adding SUVSD in a logistic regression model using a 50/50 data partition for training and testing significantly increased the mean AUC by 0.08, 0.06 and 0.04 in the low-, medium- and high-dose regions, respectively. Conclusion: Addition of SUVSD from a pre-therapy PET scan to a single CT-based texture feature improves RP-classification performance on average. These findings demonstrate the potential for more accurate prediction of RP using information from multiple imaging modalities. Supported, in part, by the National Institute of Biomedical Imaging and Bioengineering of the National Institutes of Health under grant number T32 EB002103; SGA receives royalties and licensing fees through the University of Chicago for computer-aided diagnosis technology. HA receives royalties through the University of Chicago for computer-aided diagnosis technology.

  6. 76 FR 61558 - Airworthiness Directives; Dowty Propellers Type R212/4-30-4/22 and R251/4-30-4/49 Propeller...

    Science.gov (United States)

    2011-10-05

    ... published in the Federal Register on May 11, 2011 (76 FR 27281). That NPRM proposed to correct an unsafe... Register on May 11, 2011 (76 FR 27281) or on the determination of the cost to the public. Since we published the NPRM in the Federal Register on May 11, 2011 (76 FR 27281), we changed the AD Docket No....

  7. 76 FR 27281 - Airworthiness Directives; Dowty Propellers Type R212/4-30-4/22 and R251/4-30-4/49 Propeller...

    Science.gov (United States)

    2011-05-11

    ... Statement in the Federal Register published on April 11, 2000 (65 FR 19477-78). Discussion The European... Policies and Procedures (44 FR 11034, February 26, 1979); and 3. Will not have a significant...

  8. Neutron multiplicities for the transplutonium nuclides

    International Nuclear Information System (INIS)

    This paper continues, with respect to the transplutonium nuclides, earlier efforts to collate and evaluate data from the scientific literature on the prompt neutron multiplicity distribution from fission and its first moment = ΣnuPnu. The isotopes considered here for which P/sub nu/ and or data (or both) were found in the literature are of americium (Am), curium (Cm), berkelium (Bk), californium (Cf), einsteinium (Es), fermium (Fm), and nobelium (No)

  9. A t(3;9)(q25.1;q34.3) translocation leading to OLFM1 fusion transcripts in Gilles de la Tourette syndrome, OCD and ADHD

    DEFF Research Database (Denmark)

    Bertelsen, Birgitte; Melchior, Linea; Jensen, Lars Riff;

    2015-01-01

    could be a contributing risk factor to the phenotype of the carriers. In addition, one of the fusion transcripts may exert a dominant-negative or gain-of-function effect. OLFM1 is unlikely to be a major GTS susceptibility gene as no point mutations or copy number variants affecting OLFM1 were identified...... transcripts. Reverse-transcription PCR identified several fusion transcripts in the carriers, and OLFM1 expression was found to be high in GTS-related human brain regions. As OLFM1 plays a role in neuronal development it is a likely candidate gene for neuropsychiatric disorders and haploinsufficiency of OLFM1...

  10. Erratum to ``Eruption style and petrology of a new carbonatitic suite from the Mt. Vulture (Southern Italy): The Monticchio Lakes Formation'' [Journal of Volcanology and Geothermal Research 78 (1997) 251 265

    Science.gov (United States)

    Stoppa, Francesco; Principe, Claudia

    1998-01-01

    The Monticchio Lakes Formation (MLF) is a newly identified carbonatite-melilitite tuff sequence which is exposed in the southwestern sector of the Vulture volcano. It is the youngest example (ca. 0.13 m.y.) of this type of volcanism in Italy, although other carbonatites of smaller volume, but with similar characteristics, have been discovered recently. This volcanic event occurred in isolation after a 0.35 m.y. period of inactivity at Vulture. The eruption produced two maar-type vents and formed tuff aprons mainly composed of dune beds of lapilli. Depositional features suggest that a dry surge mechanism, possibly triggered by CO 2 expansion, was dominant during tuff emplacement. The MLF event involved a mixture of carbonatite and melilitite liquids which were physically separated before the eruption. Abundant mantle xenoliths are direct evidence of the deep-seated origin of the parental magma and its high velocity of propagation towards the surface. Often, these nodules form the core of lapilli composed of concentric shells of melilitite and/or porphyritic carbonatite. Coarse-ash beds alternate with lapilli beds and consist of abundant lumps and spherulae of very fine-grained calcite immersed in a welded, highly compacted carbonatite matrix. Porphyritic carbonatite shells of the lapilli and fine-grained spherulae of calcite in the tuff matrix suggest incipient crystallisation of a carbonatite liquid in subvolcanic conditions and eruption of carbonatite-spray droplets. Dark coloured juvenile fragments mainly consist of melilite, phlogopite, calcite, apatite, perovskite, and häuyne crystals in a carbonatite or melilitite matrix. The rocks have an extremely primitive, ultramafic composition with very high Mg# (> 85) and Cr and Ni content (1500 ppm). The calcite contains high SrO, BaO and REE of up to 1.5 wt.%. Similar compositions are typical of primary, magmatic carbonates which are found in both intrusive and extrusive carbonatites. The high modal Sr-Ba-REE-rich calcite, the typical mineralogy, and the high amount of Sr-group elements identify the carbonate component as a carbonatite. The very high Mg#, mantle debris and C, O, He isotope ratios in the range of mantle values indicate a near-primary character for the carbonatite which is distinctive of a restricted group of extrusive carbonatites only found in continental rift areas.

  11. “The Woman Warrior versus The Chinaman Pacific: Must a Chinese American Critic Choose between Feminism and Heroism?” Conflicts in Feminism. Ed. Marianne Hirsch and Evelyn Fox Keller. New York: Routledge, 1990. 234-251

    OpenAIRE

    Cheung, KK

    1991-01-01

    The chapter examines the tension between white feminism and ethnic feminism as well as between men and women in Asian American literary circles. Specifically it addresses Frank Chin’s attack on Maxine Hong Kingston over her depiction of Chinese sexism in The Woman Warrior and my own position as a literary critic torn between cultural nationalism and feminism. Using the opening of Kingston’s “China Men” as my point of departure, I urge Asian American men who feel “emasculated” by white culture...

  12. REFLEXÕES SOBRE A ESTRATÉGIA DO OCEANO AZUL: UMA COMPARAÇÃO COM AS ESTRATÉGIAS DE ANSOFF, PORTER E HAX & WILDE DOI:10.7444/fsrj.v2i2.51

    OpenAIRE

    Ailton Conde Jussani; Patricia Viveiros de Castro Krakauer; Edison Fernandes Polo

    2010-01-01

    Within the context of the globalized environment, competitiveness has become a critical issue for business. The use of research to inform strategic decisions is thus important for firms on the path to competitiveness, regardless of their market of operation. This paper provides an overview of four strategies—Kim and Mauborgne’s Blue Ocean Strategy, Ansoff’s Matrix, Porter’s Generic Strategies, and Hax and Wilde’s Delta model—in order to find the similarities and approximations among them. App...

  13. RESGPSPNTS_JD251.SHP: Navigation, Bathymetry and Temperature Point at the Ship Position During Continuous Resistivity Profile Data Collection in the Potomac River/Chesapeake Bay on Sept. 8, 2006 on USGS Cruise 06018

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — In order to test hypotheses about groundwater flow under and into Chesapeake Bay, geophysical surveys were conducted by U.S. Geological Survey (USGS) scientists on...

  14. Genetic variants at chromosomes 2q35, 5p12, 6q25.1, 10q26.13, and 16q12.1 influence the risk of breast cancer in men.

    OpenAIRE

    Nick Orr; Rosie Cooke; Michael Jones; Olivia Fletcher; Frank Dudbridge; Sarah Chilcott-Burns; Katarzyna Tomczyk; Peter Broderick; Richard Houlston; Alan Ashworth; Anthony Swerdlow

    2011-01-01

    Male breast cancer accounts for approximately 1% of all breast cancer. To date, risk factors for male breast cancer are poorly defined, but certain risk factors and genetic features appear common to both male and female breast cancer. Genome-wide association studies (GWAS) have recently identified common single nucleotide polymorphisms (SNPs) that influence female breast cancer risk; 12 of these have been independently replicated. To examine if these variants contribute to male breast cancer ...

  15. Genetic Variants at Chromosomes 2q35, 5p12, 6q25.1, 10q26.13, and 16q12.1 Influence the Risk of Breast Cancer in Men

    OpenAIRE

    Orr, N.; Cooke, R; Jones, M.(Purdue University, West Lafayette, U.S.A.); Fletcher, O.; Dudbridge, F; Chilcott-Burns, S; Tomczyk, K; Broderick, P.; Houlston, R.; Ashworth, A.; Swerdlow, A.

    2011-01-01

    Male breast cancer accounts for approximately 1% of all breast cancer. To date, risk factors for male breast cancer are poorly defined, but certain risk factors and genetic features appear common to both male and female breast cancer. Genome-wide association studies (GWAS) have recently identified common single nucleotide polymorphisms (SNPs) that influence female breast cancer risk; 12 of these have been independently replicated. To examine if these variants contribute to male breast cancer ...

  16. 76 FR 62694 - Appeal of Decisions Relating to Occupancy or Use of National Forest System Lands and Resources

    Science.gov (United States)

    2011-10-11

    ... appeal rule at 36 CFR part 251, subpart C (54 FR 3362) (the 251 Appeal Rule). The 251 Appeal Rule set... Forest Service 36 CFR Parts 212, 214, 215, 218, 222, 228, 241, 251, 254, and 292 RIN 0596--AB45 Appeal of Decisions Relating to Occupancy or Use of National Forest System Lands and Resources AGENCY: USDA,...

  17. Substituição do milho por farelo de trigo ou farelo de glúten de milho na ração de bovinos de corte em terminação - DOI: 10.4025/actascianimsci.v29i1.251 Substitution of corn grain by wheat middlings or corn gluten feed in the finishing bulls diet - DOI: 10.4025/actascianimsci.v29i1.251

    Directory of Open Access Journals (Sweden)

    Térssio Roger Ramalho

    2007-11-01

    Full Text Available O objetivo do trabalho foi avaliar a substituição do milho moído fino (MM por farelo de trigo (FT ou farelo de glúten de milho (FGM na ração de 36 bovinos não castrados, em terminação (14 meses; peso inicial de 382 kg durante 87 dias. A ração continha 24% de silagem de capim e 76% de concentrado, constituído por polpa cítrica, farelo de soja, uréia, minerais, bicarbonato de sódio e monensina sódica, além de uma das fontes energéticas testadas. A substituição de MM por FT aumentou a ingestão de FDN (pThe objective of this work was to evaluate the substitution of finely ground (GC corn by wheat middlings (WM or dried corn gluten feed (CGF in finishing feedlot diets. Thirty-six yearling bulls (382 kg average initial live weight; 14 months old were fed during 87 days. The diets contained 24% grass silage and 76% concentrate, formulated with dried citrus pulp, soybean meal, urea, mineral-vitamin mix, sodium bicarbonate, sodium monensin, and one of the energy sources tested. The substitution of wheat middlings for corn increased NDF intake (p<0.05. However, no effect was observed on other measured parameters. Substitution of corn gluten feed for ground corn reduced dry matter intake (p<0.05 and average daily gain (p<0.10, increased NDF intake (p<0.05, but had no effect on feed efficiency. These diets showed no differences in the observed values of net energy of maintenance or gain. The ratio observed/expected net energy for the diet differs from the other treatments. For this type of diet, GC may be completely replaced by WM or CGF with no depression on feed efficiency or on diet net energy. NRC (2001 underestimates TDN values and net energy estimates for WM and CGF.

  18. TOWARD AN IMPROVED UNDERSTANDING OF STRUCTURE AND MAGNETISM IN NEPTUNIUM AND PLUTONIUM PHOSPHONATES AND SULFONATES

    Energy Technology Data Exchange (ETDEWEB)

    Albrecht-Schmitt, Thomas

    2012-03-01

    This grant supported the exploratory synthesis of new actinide materials with all of the actinides from thorium to californium with the exceptions of protactinium and berkelium. We developed detailed structure-property relationships that allowed for the identification of novel materials with selective ion-exchange, selective oxidation, and long-range magnetic ordering. We found novel bonding motifs and identified periodic trends across the actinide series. We identified structural building units that would lead to desired structural features and novel topologies. We also characterized many different spectroscopic trends across the actinide series. The grant support the preparation of approximately 1200 new compounds all of which were structurally characterized.

  19. Composition containing transuranic elements for use in the homeopathic treatment of aids

    International Nuclear Information System (INIS)

    A homeopathic remedy consisting of a composition containing one or more transuranic elements, particularly plutonium, for preventing and treating acquired immunodeficiency syndrome (AIDS) in humans, as well as seropositivity for human immunodeficiency virus (HIV). Said composition is characterized in that it uses any chemical or isotopic form of one or more transuranic elements (neptunium, plutonium, americium, curium, berkelium, californium or einsteinium), particularly plutonium, said form being diluted and dynamized according to conventional homeopathic methods, particularly the so-called Hahnemann and Korsakov methods, and provided preferably but not exclusively in the form of lactose and/or saccharose globules or granules impregnated with the active principle of said composition. (author)

  20. Detection of rare earth elements in Powder River Basin sub-bituminous coal ash using laser-induced breakdown spectroscopy (LIBS)

    Energy Technology Data Exchange (ETDEWEB)

    Tran, Phuoc [National Energy Technology Lab. (NETL), Pittsburgh, PA, (United State; Mcintyre, Dustin [National Energy Technology Lab. (NETL), Pittsburgh, PA, (United State

    2015-10-01

    We reported our preliminary results on the use of laser-induced breakdown spectroscopy to analyze the rare earth elements contained in ash samples from Powder River Basin sub-bituminous coal (PRB-coal). We have identified many elements in the lanthanide series (cerium, europium, holmium, lanthanum, lutetium, praseodymium, promethium, samarium, terbium, ytterbium) and some elements in the actinide series (actinium, thorium, uranium, plutonium, berkelium, californium) in the ash samples. In addition, various metals were also seen to present in the ash samples

  1. PROCEEDINGS OF THE SYMPOSIUM COMMEMORATING THE 25th ANNIVERSARY OF ELEMENTS 97 and 98 HELD ON JAN. 20, 1975

    Energy Technology Data Exchange (ETDEWEB)

    Seaborg, Glenn T.; Street Jr., Kenneth; Thompson, Stanley G.; Ghiorso, Albert

    1976-07-01

    This volume includes the talks given on January 20, 1975, at a symposium in Berkeley on the occasion of the celebration of the 25th anniversary of the discovery of berkelium and californium. Talks were given at this symposium by the four people involved in the discovery of these elements and by a number of people who have made significant contributions in the intervening years to the investigation of their nuclear and chemical properties. The papers are being published here, without editing, in the form in which they were submitted by the authors in the months following the anniversary symposium, and they reflect rather faithfully the remarks made on that occasion.

  2. Dicty_cDB: Contig-U14270-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available |||||||||| Sbjct: 191 gttcaacacaatgcngntcngcaacatgctganccagntgccaagccagccgaattaacc 250 Query: 251 natgcccnagaattncaacaagaacaccna...||||||| Sbjct: 251 natgcccnagaattncaacaagaacaccnaagncaagnagctctcgcaaaacnncnngct 3...10 Query: 311 cgtattagncaacaaaatcntgtcaaacaagcngcccnanagaaacgaacctcgcnacgt 370 |||||||||||||||||||||||||||||...||||||||||||||||||||||||||||||| Sbjct: 311 cgtattagncaacaaaatcntgtcaaacaagcngcccnanagaaacgaacctcgcnacgt 370 ...ct: 219 cngctgctcngggggctgccaanccagttcaacacaatgcngntcngcaacatgctganc 160 Query: 225 cagntgccaagccagccgaattaaccnatgcccna

  3. 78 FR 33705 - Postdecisional Administrative Review Process for Occupancy or Use of National Forest System Lands...

    Science.gov (United States)

    2013-06-05

    ..., the Department adopted an administrative appeal rule at 36 CFR part 251, subpart C (54 FR 3362) (251... Administrative Review Process for Occupancy or Use of National Forest System Lands and Resources AGENCY: USDA... and is not part of the administrative review process under the 251 Appeal Rule. Public Involvement...

  4. An atomic beam source for actinide elements: concept and realization

    International Nuclear Information System (INIS)

    For ultratrace analysis of actinide elements and studies of their atomic properties with resonance ionization mass spectroscopy (RIMS), efficient and stable sources of actinide atomic beams are required. The thermodynamics and kinetics of the evaporation of actinide elements and oxides from a variety of metals were considered, including diffusion, desorption, and associative desorption. On this basis various sandwich-type filaments were studied. The most promising system was found to consist of tantalum as the backing material, an electrolytically deposited actinide hydroxide as the source of the element, and a titanium covering layer for its reduction to the metal. Such sandwich sources were experimentally proven to be well suited for the production of atomic beams of plutonium, curium, berkelium and californium at relatively low operating temperatures and with high and reproducible yields. (orig.)

  5. EC decay of 244Bk

    Science.gov (United States)

    Sodaye, Suparna; Tripathi, R.; Sudarshan, K.; Sharma, S. K.; Pujari, P. K.; Palit, R.; Mukhopadhyay, S.

    2014-12-01

    Berkelium isotopes have been produced in 11B-induced reaction on 238U. The EC decay of 244Bk → 244Cm has been studied by carrying out the single and coincidence measurements of the γ-rays emitted during the de-excitation of the 244Cm levels. Radiochemical separations have been carried out to minimize the contribution from the fission products and target. The new half-life of 244Bk is obtained as 5.02 ± 0.03 h, which is close to the theoretically calculated value. The relative intensities of the decay γ-rays have been re-evaluated. Based on the coincidence measurements, a tentative partial level scheme for 244Bk → 244Cm decay has been proposed.

  6. Nuclear fission and the transuranium elements

    International Nuclear Information System (INIS)

    Many of the transuranium elements are produced and isolated in large quantities through the use of neutrons furnished by nuclear fission reactions: plutonium (atomic number 94) in ton quantities; neptunium (93), americium (95), and curium (96) in kilogram quantities; berkelium (97) in 100 milligram quantities; californium (98) in gram quantities; and einsteinium (99) in milligram quantities. Transuranium isotopes have found many practical applications---as nuclear fuel for the large-scale generation of electricity, as compact, long-lived power sources for use in space exploration, as means for diagnosis and treatment in the medical area, and as tools in numerous industrial processes. Of particular interest is the unusual chemistry and impact of these heaviest elements on the periodic table. This account will feature these aspects. 9 refs., 5 figs

  7. Nuclear fission and the transuranium elements

    Energy Technology Data Exchange (ETDEWEB)

    Seaborg, G.T.

    1989-02-01

    Many of the transuranium elements are produced and isolated in large quantities through the use of neutrons furnished by nuclear fission reactions: plutonium (atomic number 94) in ton quantities; neptunium (93), americium (95), and curium (96) in kilogram quantities; berkelium (97) in 100 milligram quantities; californium (98) in gram quantities; and einsteinium (99) in milligram quantities. Transuranium isotopes have found many practical applications---as nuclear fuel for the large-scale generation of electricity, as compact, long-lived power sources for use in space exploration, as means for diagnosis and treatment in the medical area, and as tools in numerous industrial processes. Of particular interest is the unusual chemistry and impact of these heaviest elements on the periodic table. This account will feature these aspects. 9 refs., 5 figs.

  8. EC decay of 244Bk

    International Nuclear Information System (INIS)

    Berkelium isotopes have been produced in 11B-induced reaction on 238U. The EC decay of 244Bk → 244Cm has been studied by carrying out the single and coincidence measurements of the γ-rays emitted during the de-excitation of the 244Cm levels. Radiochemical separations have been carried out to minimize the contribution from the fission products and target. The new half-life of 244Bk is obtained as 5.02 ± 0.03 h, which is close to the theoretically calculated value. The relative intensities of the decay γ-rays have been re-evaluated. Based on the coincidence measurements, a tentative partial level scheme for 244Bk → 244Cm decay has been proposed. (paper)

  9. Production of transuranium elements

    International Nuclear Information System (INIS)

    The Radiochemical Engineering Development Center (REDC) has the programmatic responsibility for the Department of Energy's Transuranium Element Program. Principle elements from the program are einsteinium, berkelium, and fermium. Targets containing curium oxide mixed with aluminum powder are fabricated by the REDC and irradiated in the Oak Ridge National Laboratory (ORNL) High Flux Isotope Reactor. Following an irradiation period of 6-12 months, targets are returned to the REDC for chemical processing. Processing operations consists of aluminum dejacketing in a caustic-nitrate solution, filtration, acid dissolution, solvent extraction, anion exchange, and finally a cation exchange to recover the actinides. The processing operations take place in heavily shielded hot cell facilities and all operations are carried out remotely. The chemistry for the separations has been well established over the 26-yr. operating life of the facility

  10. The Launch of a Joint Library/Writing Centre Online Course on Academic Integrity. A Review of: Greer, K., Swanberg, S., Hristova, M., Switzer, A. T., Daniel, D., & Perdue, S. W. (2012. Beyond the web tutorial: Development and implementation of an online, self-directed academic integrity course at Oakland University. The Journal of Academic Librarianship, 38(5, 251-258.

    Directory of Open Access Journals (Sweden)

    Cari Merkley

    2013-06-01

    Full Text Available Objective – To outline the collaborative development of an online course addressing academic integrity by a university’s library system and writing centre.Design – Case study.Setting – A public research university in the Midwestern United States.Subjects – 1650 students who completed the online module.Methods – Oakland University (OU Libraries and the Writing Centre began to collaborate on the development of a new online course on academic integrity in 2011. It was felt that an existing online library tutorial on plagiarism no longer met the needs of students and faculty. The development of the course was informed by the Association of College and Research Libraries’ Information Literacy Competency Standards for Higher Education (2000 as well as a research study investigating students’ use of sources in their scholarly writing across several institutions. Moodle, the institution’s learning management system (LMS, was used to develop the learning object.Main Results – OU Libraries and the Writing Centre launched the six-part online course entitled “Using and Citing Sources” in January 2012. They developed modules around learning outcomes in five broad categories: defining academic integrity and plagiarism; the use of sources in academic writing; paraphrasing; quoting; and citation. The final module provided students with an opportunity to practise lessons learned in the first five modules. The use of the LMS to design and host the course limited the tutorial to registered students, but provided developers with access to additional course functionality without labour-intensive coding. It also allowed Writing Centre staff to access students’ performance data on the modules prior to their appointments. Improvements over the previous online tutorial included expanded content on academic ethics and referencing, more active learning elements, video content, and the opportunity for students to choose discipline-specific examples. In the first four months of its availability, 1650 students completed the course, with 3330 attempts overall.Conclusion – The diverse perspectives and expertise that individuals from OU Libraries and the Writing Center brought to their collaboration greatly informed the development of the course. The time and effort saved by using the university’s existing LMS to develop interactive content and the focus on providing students with opportunities for active learning within the course contributed to the project’s success.

  11. Physical trajectory profile data from glider unit_251 deployed by Oregon Health & Science University; Center for Coastal Margin Observation & Prediction in the Coastal Waters of Washington/Oregon from 2013-07-18 to 2013-08-06 (NCEI Accession 0145922)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Slocum glider dataset gathered as part of the CMOP observatory SATURN, funded by NOAA and NSF. Glider operations have historically been seasonal (April-September),...

  12. Physical trajectory profile data from glider unit_251 deployed by Oregon Health & Science University; Center for Coastal Margin Observation & Prediction in the Coastal Waters of Washington/Oregon from 2013-05-08 to 2013-05-31 (NCEI Accession 0145921)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Slocum glider dataset gathered as part of the CMOP observatory SATURN, funded by NOAA and NSF. Glider operations have historically been seasonal (April-September),...

  13. Physical trajectory profile data from glider unit_251 deployed by Oregon Health & Science University; Center for Coastal Margin Observation & Prediction in the Coastal Waters of Washington/Oregon from 2012-07-25 to 2012-08-22 (NCEI Accession 0145920)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Slocum glider dataset gathered as part of the CMOP observatory SATURN, funded by NOAA and NSF. Glider operations have historically been seasonal (April-September),...

  14. Nr 251 - Report on the behalf of the Commission of finances, general economy and budget control on the finance bill project for 2013. Appendix Nr 13: ecology, sustainable development and planning, risk prevention, management and steering of policies of ecology, energy, sustainable development and sea

    International Nuclear Information System (INIS)

    After having indicated some key figures (public finances and budgets awarded to different involved institutions and agencies), this report comments and discusses the various challenges and financial aspects regarding risk prevention: evolution of endowments in 2013, action in the field of prevention of technological risks and pollutions, issue of nuclear safety after the Fukushima accident (actions undertaken by the ASN, IRSN and ANDRA), assessment of the implementation of the PPRN (plan of prevention of natural risks) and management of flood risks, management of the after-mine period. The second part discusses the management and steering of policies of ecology, energy, sustainable development and sea in a context of decreased endowments

  15. Dietary exposure to benzoates (E210-E213), parabens (E214-E219), nitrites (E249-E250), nitrates (E251-E252), BHA (E320), BHT (E321) and aspartame (E951) in children less than 3 years old in France.

    Science.gov (United States)

    Mancini, F R; Paul, D; Gauvreau, J; Volatier, J L; Vin, K; Hulin, M

    2015-01-01

    This study aimed to estimate the exposure to seven additives (benzoates, parabens, nitrites, nitrates, BHA, BHT and aspartame) in children aged less than 3 years old in France. A conservative approach, combining individual consumption data with maximum permitted levels, was carried out for all the additives. More refined estimates using occurrence data obtained from products' labels (collected by the French Observatory of Food Quality) were conducted for those additives that exceeded the acceptable daily intake (ADI). Information on additives' occurrence was obtained from the food labels. When the ADI was still exceeded, the exposure estimate was further refined using measured concentration data, if available. When using the maximum permitted level (MPL), the ADI was exceeded for benzoates (1.94 mg kg(-1) bw day(-1)), nitrites (0.09 mg kg(-1) bw day(-1)) and BHA (0.39 mg kg(-1) bw day(-1)) in 25%, 54% and 20% of the entire study population respectively. The main food contributors identified with this approach were current foods as these additives are not authorised in specific infant food: vegetable soups and broths for both benzoates and BHA, delicatessen and meat for nitrites. The exposure estimate was significantly reduced when using occurrence data, but in the upper-bound scenario the ADI was still exceeded significantly by the age group 13-36 months for benzoates (2%) and BHA (1%), and by the age group 7-12 months (16%) and 13-36 months (58%) for nitrites. Measured concentration data were available exclusively for nitrites and the results obtained using these data showed that the nitrites' intake was below the ADI for all the population considered in this study. These results suggest that refinement of exposure, based on the assessment of food levels, is needed to estimate the exposure of children to BHA and benzoates for which the risk of exceeding the ADI cannot be excluded when using occurrence data. PMID:25686474

  16. Vazba mutantní formy proteinu p53 k intronovým a intergenním sekvencím tvořícím triplex DNA reguluje expresi genů u buněk U251

    Czech Academy of Sciences Publication Activity Database

    Tichý, Vlastimil; Quante, T.; Tögel, L.; Walter, K.; Navrátilová, Lucie; Lexa, M.; Deppert, W.; Tolstonog, G.V.; Paleček, Emil; Brázdová, Marie

    Praha, 2009. s. 452. ISSN 0009-2770. [Mezioborové setkání mladých biologů, biochemiků a chemiků. Konference Sigma-Aldrich /9./. 26.05.2009-29.05.2009, Devět skal - Žďárské vrchy] R&D Projects: GA MŠk(CZ) 1K04119; GA MŠk(CZ) LC06035; GA ČR(CZ) GP204/06/P369; GA ČR(CZ) GA204/08/1560; GA AV ČR(CZ) IAA500040701 Institutional research plan: CEZ:AV0Z50040507; CEZ:AV0Z50040702 Keywords : mutant p53 * gene regulation * glioblastoma cells Subject RIV: BO - Biophysics

  17. Dicty_cDB: VHH719 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available s cDN... 251 5e-65 AK145000_1( AK145000 |pid:none) Mus musculus mammary gland RCB-052... 251 5e-65 BC110850_...nsyqt*krfqnski*pqkmpshlkqfklvpiwi qsfiifqiiyqrvlntlrcw*tfslqsfeislini*risissylpwpqitsii*sivkin slrnqkqsvlkpyyslmmal...inkfr--- ---**sklnqnftknikyfrenkeqlnnmypselvtelerdirqlgvsetgltfldhfrl lithignamgyirlvrsgglhycsnaikfvpdlkkipkfqdltskdals...e: Full=UPF0681 protein KIAA1033; 251 5e-65 AK161247_1( AK161247 |pid:none) Mus musculus adult male testi...petiqastnld siihnlsnnlsegteyfkmlvnvfatefrnianqhlknfyiivpalasnyidhminskdk lfkkskaigaeallftddgfaigl

  18. Dicty_cDB: Contig-U14105-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available rxirnfkqqvdsgvisldenkwiaigskiftxvsetikalxdixladlsfrlylqa lqtfdhcglvsrvkelaikallifqediadfxaqvmalqllixtfn*fhhskfhhsklis tcs...tagctttagattataccttcaagcactccaaacct 190 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct...: 131 tgnccgatatcnaattggccgatcttagctttagattataccttcaagcactccaaacct 190 Query: 191 ttgaccactgtggtttggtctctc...gtgtcaaagaattggccatcaaggctctcttaatct 250 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct...: 191 ttgaccactgtggtttggtctctcgtgtcaaagaattggccatcaaggctctcttaatct 250 Query: 251 tc

  19. First Genome Sequence of a Canine Distemper Virus Strain from South America

    OpenAIRE

    Sarute, Nicolás; Delgado, María V.; Carrau, Lucía; Benech, Alejandro; Francia, Lourdes; Pérez, Ruben; Panzera, Yanina

    2014-01-01

    Canine distemper virus causes a severe infectious disease in carnivores worldwide. Herein, we sequenced and analyzed the genome of a new strain (Uy251/2012) isolated from a dog in Uruguay. The Uy251/2012 strain belongs to the Europe1/South America1 lineage, and constitutes the first report of a genomic sequence in South America.

  20. Unigene BLAST: CBRC-CELE-05-0834 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-CELE-05-0834 gnl|UG|Cel#S5712016 Caenorhabditis elegans Serpentine Receptor, c...lass H family member (srh-251) (srh-251) mRNA, complete cds /cds=p(1,963) /gb=NM_074418 /gi=17562995 /ug=Cel.27436 /len=963 1e-135 71% ...

  1. Fast Neutron Induced Autophagy Leads To Necrosis In Glioblastoma Multiforme Cells

    International Nuclear Information System (INIS)

    Fast neutrons are highly effective at killing glioblastoma multiforme (GBM), U87 and U251 cells. The mode of cell death was investigated using transmission electron microscopy (TEM) to identify the fraction of irradiated U87 or U251 cells having morphological features of autophagy and/or necrosis. U87 or U251 cells were irradiated with 2 Gy fast neturons or 10 Gy γ rays. A majority of U87 and U251 cells exhibit features of cell death with autophagy after irradiation with either 10 Gy γ rays or 2 Gy fast neutrons. Very few γ irradiated cells had features of necrosis (U87 or U251 cell samples processed for TEM 1 day after 10 Gy γ irradiation). In contrast, a significant increase was observed in necrotic U87 and U251 cells irradiated with fast neutrons. These results show a greater percentage of cells exhibit morphological evidence of necrosis induced by a lower dose of fast neutron irradiation compared to γ irradiation. Also, the evidence of necrosis in fast neutron irradiated U87 and U251 cells occurs in a background of autophagy. Since autophagy is observed before necrosis, autophagy may play a role in signaling programmed necrosis in fast neutron irradiated U87 and U251 cells.

  2. Fast Neutron Induced Autophagy Leads To Necrosis In Glioblastoma Multiforme Cells

    Science.gov (United States)

    Yasui, Linda; Gladden, Samantha; Andorf, Christine; Kroc, Thomas

    2011-06-01

    Fast neutrons are highly effective at killing glioblastoma multiforme (GBM), U87 and U251 cells. The mode of cell death was investigated using transmission electron microscopy (TEM) to identify the fraction of irradiated U87 or U251 cells having morphological features of autophagy and/or necrosis. U87 or U251 cells were irradiated with 2 Gy fast neturons or 10 Gy γ rays. A majority of U87 and U251 cells exhibit features of cell death with autophagy after irradiation with either 10 Gy γ rays or 2 Gy fast neutrons. Very few γ irradiated cells had features of necrosis (U87 or U251 cell samples processed for TEM 1 day after 10 Gy γ irradiation). In contrast, a significant increase was observed in necrotic U87 and U251 cells irradiated with fast neutrons. These results show a greater percentage of cells exhibit morphological evidence of necrosis induced by a lower dose of fast neutron irradiation compared to γ irradiation. Also, the evidence of necrosis in fast neutron irradiated U87 and U251 cells occurs in a background of autophagy. Since autophagy is observed before necrosis, autophagy may play a role in signaling programmed necrosis in fast neutron irradiated U87 and U251 cells.

  3. Review: Derek Hook, A Critical Psychology of the Postcolonial: The Mind of Apartheid (2012 Buchbesprechung: Derek Hook, A Critical Psychology of the Postcolonial: The Mind of Apartheid (2012

    Directory of Open Access Journals (Sweden)

    Danielle Faye Tran

    2013-01-01

    Full Text Available Review of the monograph:Derek Hook, A Critical Psychology of the Postcolonial: The Mind of Apartheid, London: Routledge, 2012, ISBN 978-0-415-58757-0, 251 pagesBesprechung der Monographie:Derek Hook, A Critical Psychology of the Postcolonial: The Mind of Apartheid, London: Routledge, 2012, ISBN 978-0-415-58757-0, 251 Seiten

  4. Lucifensins, the Insect Defensins of Biomedical Importance: The Story behind Maggot Therapy

    Czech Academy of Sciences Publication Activity Database

    Čeřovský, Václav; Bém, R.

    2014-01-01

    Roč. 7, č. 3 (2014), s. 251-264. ISSN 1424-8247 R&D Projects: GA ČR GA203/08/0536 Institutional support: RVO:61388963 Keywords : antimicrobial peptide * insect defensin * lucifensin * maggot therapy * Lucilia sericata * Lucilia cuprina * peptide isolation * peptide identification Subject RIV: CC - Organic Chemistry http://www.mdpi.com/1424-8247/7/3/251

  5. Processes determining seasonality and interannual variability of settling particle fluxes to the deep Arabian Sea

    Digital Repository Service at National Institute of Oceanography (India)

    Haake, B.; Rixen, T.; Reemtsma, T.; Ramaswamy, V.; Ittekkot, V.

    stream_size 20 stream_content_type text/plain stream_name Particle_Flux_Ocean_Chapter_14_1996_251.pdf.txt stream_source_info Particle_Flux_Ocean_Chapter_14_1996_251.pdf.txt Content-Encoding ISO-8859-1 Content-Type text...

  6. EST Table: FS808440 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available FS808440 E_FL_fmgV_26F15_F_0 10/09/28 60 %/251 aa ref|NP_001116815.1| carboxylesterase CarE -11 [ ... Bombyx mori] gb|ACB12415.1| carboxylesterase CarE -11 [Bombyx mori] 10/09/09 42 %/251 aa FBpp0081074| ...

  7. 78 FR 38842 - Definition of a Ski Area

    Science.gov (United States)

    2013-06-28

    ... definition for a ski area in Forest Service regulations at 36 CFR 251.51 implementing the National Forest Ski..., the Department is amending the definition for a ski area in 36 CFR 251.51 to provide for development....51 that the preponderance of revenue at a ski area derive from activities and facilities that...

  8. 76 FR 41075 - Copyright Arbitration Royalty Panel Rules and Procedures

    Science.gov (United States)

    2011-07-13

    ... Copyright Office 37 CFR Part 251 Copyright Arbitration Royalty Panel Rules and Procedures AGENCY: Copyright... making an amendment to its regulations by removing Part 251 Copyright Arbitration Royalty Panel Rules of Procedure. In 2004, Congress replaced the Copyright Arbitration Royalty Panels with three Copyright...

  9. Review: Derek Hook, A Critical Psychology of the Postcolonial: The Mind of Apartheid (2012) Buchbesprechung: Derek Hook, A Critical Psychology of the Postcolonial: The Mind of Apartheid (2012)

    OpenAIRE

    Danielle Faye Tran

    2013-01-01

    Review of the monograph:Derek Hook, A Critical Psychology of the Postcolonial: The Mind of Apartheid, London: Routledge, 2012, ISBN 978-0-415-58757-0, 251 pagesBesprechung der Monographie:Derek Hook, A Critical Psychology of the Postcolonial: The Mind of Apartheid, London: Routledge, 2012, ISBN 978-0-415-58757-0, 251 Seiten

  10. Gclust Server: 119743 [Gclust Server

    Lifescience Database Archive (English)

    Full Text Available 119743 CEL_K06A5.7_17506269 Cluster Sequences Related Sequences(24) 604 cdc-25.1: Cell Division...nces Related Sequences(24) Sequence length 604 Representative annotation cdc-25.1: Cell Division

  11. Hemp (Cannabis sativa L.) seeds after plasma treatment

    Czech Academy of Sciences Publication Activity Database

    Šerá, Božena; Gajdová, Iveta; Gavril, B.; Hnatiuc, E.; Šerý, M.; Špatenka, P.

    Brasov: Universitatea Transilvania din Brasov, 2012 - (Clotea, L.; Cernat, M.). s. 251-251 ISBN 978-1-4673-1650-7. [International Conference on Optimization of Electrical and Electronic Equipment /13./ OPTIM 2012. 24.05.-26.05.2012, Brasov] Institutional support: RVO:67179843 Keywords : Cannabis sativa L. * plasma treatment Subject RIV: EI - Biotechnology ; Bionics

  12. 37 CFR 270.2 - Reports of use of sound recordings under statutory license for preexisting subscription services.

    Science.gov (United States)

    2010-07-01

    ... Code and adopted pursuant to 37 CFR 251.63(b), or by decision of a Copyright Arbitration Royalty Panel... section 114(f)(1)(C)(i) and adopted pursuant to 37 CFR 251.63(b), or by decision of a Copyright... recording copyright owner; and (2) A “click-wrap” agreement not to use information in the Report of Use...

  13. Establishment of Drug-resistance Cell Line of Human Glioma Mediated by MGMT%MGMT介导的人脑胶质瘤耐药细胞系的建立

    Institute of Scientific and Technical Information of China (English)

    金澎; 张庆林; 刘福生; 王保安; 魏麟; 王成伟; 孔建新

    2002-01-01

    目的建立由O6-甲基鸟嘌呤-DNA甲基转移酶(MGMT)介导的人脑胶质瘤耐药细胞株U251/BCNU.方法模拟卡氮芥(BCNU)的临床用药程序,采取恒定药物浓度、周期性作用的方式诱导U251的抗药性.检测U251/BCNU的耐药指数及MGMT mRNA的表达;比较U251及U251/BCNU细胞的体外增殖变化.结果经过反复总共5次的用药过程,历时4个月,成功地建立了对BCNU具有稳定抗药性的U251/BCNU,其对BCNU的耐受程度约为U251的17倍.RT-PCR显示,U251/BCNU细胞有MGMTmRNA的表达.U251及U251/BCNU细胞的体外群体倍增时间差异无显著性.结论在体外成功地建立了一株由MGMT介导的人脑胶质瘤耐药细胞系,为进一步探讨胶质瘤的耐药机制及逆转方式奠定了基础.

  14. Nuclear Chemistry Institute, Mainz University. Annual Report 1995

    International Nuclear Information System (INIS)

    The annual report of the Institut fuer Kernchemie addresses inter alia three main research activities. The first belongs to the area of basic research, covering studies in the fields of nuclear fission, chemistry of the super-heavy elements and of heavy-ion reactions extending from the Coulomb barrier to relativistic energies, and nuclear astrophysics in connection with the ''r process''. By means of laser technology, high-precision data could be measured of the ionization energies of berkelium and californium. Studies of atomic clusters in the vacuum of an ionization trap revealed interesting aspects. The second major activity was devoted to the analysis of environmental media, applying inter alia neutron activation analysis and resonance ionization mass spectroscopy (RIMS). The third activity resulted in the development of novel processes, or the enhancement of existing processes or methods, for applications in basic research work and in environmental analytics. Another item of interest is the summarizing report on the operation of the TRIGA research reactor. (orig./SR)

  15. Paul Scherrer Institute Scientific Report 1999. Volume I: Particles and Matter

    International Nuclear Information System (INIS)

    Although originally planned for fundamental research in nuclear physics, the particle beams of pions, muons, protons and neutrons are now used in a large variety of disciplines in both natural science and medicine. The beams at PSI have the world's highest intensities and therefore allow certain experiments to be performed, which would not be possible elsewhere. The highlight of research this year was the first-ever determination of the chemical properties of the superheavy element 107 Bohrium. This was undertaken, by an international team led by H. Gaeggeler of PSI's Laboratory for Radiochemistry. Bohrium was produced by bombarding a Berkelium target with Neon ions from the Injector I cyclotron and six atoms were detected after having passed through an online gas chromatography device. At the Laboratory for Particle Physics the focus has shifted from nuclear physics to elementary particle physics with about a fifty-fifty split between investigations of rare processes or particle decays using the high intensity muon, pion and recently also polarized neutron beams of PSI, and research at the highest energy frontier at CERN (Geneva) and DESY (Hamburg). Important space instrumentation has been contributed by the Laboratory for Astrophysics to the European Space Agency and NASA satellite programmes. The Laboratory for Micro and Nanotechnology continued to focus on research into molecular nanotechnology and SiGeC nanostructures, the latter with the aim of producing silicon based optoelectronics. Progress in 1999 in these topical areas is described in this report. A list of scientific publications in 1999 is also provided

  16. Investigations for the influence of geochemical parameters on the sorption and desorption of lanthanides and uranium onto opalinus clay as potential host rock for a repository

    International Nuclear Information System (INIS)

    The development of a disposal in deep geological formations for radioactive waste is a very important task for the future. The safety assessment for more than a hundred thousand years needs a full understanding of all processes of interaction between the radioactive waste and the surrounded formations. This work contributes to this understanding. The interaction between lanthanides (homologues of the actinides americium, curium and berkelium) / uranium and the host rock opalinus clay under influence of organic substances (NOM) have been analyzed and discussed. The complex system was split into 3 binary basic systems with the following interactions - Interactions between lanthanides / uranium and NOM - Interactions between lanthanides / uranium and the opalinus clay - Interactions between NOM and opalinus clay All binary systems can be influenced by geological parameters like pH, ion strength and competing cations. The sorption / desorption of the lanthanides onto the opalinus clay is analyzed via inductively coupled plasma mass spectrometry. For the investigation of the complexation behavior of metals with NOM we used capillary electrophoresis coupled with inductively coupled plasma mass spectrometry. Under these conditions the chosen model organic humic acid affected the sorption of the lanthanides onto opalinus clay favorably. The smaller organic compounds, which dominate in the composition of the clay organics, remobilized the metals after sorption onto clay and the sorption can be inhibited by NOM. Due to the reduced metal sorption onto Opalinus clay by NOM, a migration through the clay may be possible.

  17. Dicty_cDB: Contig-U15736-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available length - Start point - End point - Strand (PLUS/MINUS) - Number of clones 23 Number of EST 28 Link to clone...), Expect = 0.0 Identities = 885/885 (100%) Strand = Plus / Plus Query: 175 ttaaaatgggatcagttgatacatcagtccca...tatagtttatccagaaattcgtgattttg 1059 Score = 1352 bits (682), Expect = 0.0 Identities = 682/682 (100%) Strand ... 498 bits (251), Expect = e-140 Identities = 251/251 (100%) Strand = Plus / Plus ...77 gaagaagttaa 2887 Score = 323 bits (163), Expect = 1e-87 Identities = 163/163 (100%) Strand

  18. HSV-1感染对神经胶质瘤细胞NGF和BDNF表达变化的研究%Study of NGF and BDNF expression in HSV-1 infected human glioma cells

    Institute of Scientific and Technical Information of China (English)

    侯云; 王斌; 李玲; 胡明; 辛晓妮; 钱冬萌; 闫志勇; 赵巍; 宋旭霞

    2011-01-01

    目的:探讨单纯疱疹病毒1型(HSV-1)感染时神经胶质细胞内源性神经生长因子(NGF)和脑源性神经生长因子(BDNF)的表达变化.方法:采用RT-PCR法检测HSV-1糖蛋白D(gD)基因扩增,RT-PCR法和Western blotting法检测正常培养和感染HSV-1的神经胶质瘤(U251)细胞的NGF和BDNF.结果:HSV-1可感染U251细胞;正常U251细胞可表达NGF和BDNF;HSV-1感染U251细胞后,NGF和BDNF在感染后第6小时达高峰,之后随感染时间延长逐渐降低.结论:HSV-1可诱导U251细胞中的NGF和BDNF表达异常.%Objective To investigate endogenic nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF) expression in herpes simplex type-1-virus (HSV-1) -infected neuroglia cells. Methods U251 human glioma cells were cultured in RPMI-I640 medium and infected with HSV-1 in vitro to estahlish a cell model of viral infection. Establishment of HSV-1-infected U251 cells was identified by HSV-1 gD mRNA expression. NGF and BDNF expression was detected by RT-PCR and Western blotting , respectively, in normal U251 cells and HSV-1-infected U251 cells. Results HSV-1 could efficiently infect U251 cells. NGF and BDNF was identified to he expressed in U251. NGF and BDNF expression reached a maximum at 6 hours post HSV-1 infection, then NGF and BDNF expression decreased in time-dependent manner. Conclusions HSV-1 infection could dysregulate NGF and BDNF expression in the U251 cells.

  19. Přípustnost matematických modelů v teorii parametraických vibrací čelních ozubených kol

    Czech Academy of Sciences Publication Activity Database

    Höschl, Cyril

    2004-01-01

    Roč. 55, č. 5 (2004), s. 251-258. ISSN 0039-2472 Institutional research plan: CEZ:AV0Z2076919 Keywords : parametric excitation * vibrations in gear trains * mathematical modelling of gear matching Subject RIV: BI - Acoustics

  20. Heteroxenous coccidia (Apicomplexa: Sarcocystidae) in the populations of their final and intermediate hosts: European buzzard and small mammals

    Czech Academy of Sciences Publication Activity Database

    Svobodová, M.; Voříšek, P.; Votýpka, Jan; Weidinger, K.

    2004-01-01

    Roč. 43, č. 3 (2004), s. 251-260. ISSN 0065-1583 Institutional research plan: CEZ:AV0Z6022909 Keywords : Coccidia * Buteo * wildlife parasites Subject RIV: EG - Zoology Impact factor: 0.986, year: 2004

  1. Sex differences in cardiovascular function

    Czech Academy of Sciences Publication Activity Database

    Kolář, František; Ošťádal, Bohuslav

    2013-01-01

    Roč. 207, č. 4 (2013), s. 584-587. ISSN 1748-1708 Institutional support: RVO:67985823 Keywords : heart * vascular * risk factors * sex Subject RIV: FA - Cardiovascular Diseases incl. Cardiotharic Surgery Impact factor: 4.251, year: 2013

  2. Innovative potential in the regions of the Czech Republic, economic, societal and regional aspects

    Czech Academy of Sciences Publication Activity Database

    Kunc, J.; Klapka, Pavel; Frantál, Bohumil

    Brno : Masarykova univerzita, 2008, s. 246-251 ISBN 978-80-210-4600-9 Institutional research plan: CEZ:AV0Z30860518 Keywords : innovative potential * correlation analysis * regions of the Czech Republic Subject RIV: AH - Economics

  3. Testing of models of stomatal ozone fluxes with field measurements in a mixed Mediterranean forest

    Czech Academy of Sciences Publication Activity Database

    Fares, S.; Matteucci, G.; Mugnozza, S.; Morani, A.; Calfapietra, Carlo; Salvatori, E.; Fusaro, L.; Manes, F.; Loreto, F.

    2013-01-01

    Roč. 67, MAR (2013), s. 242-251. ISSN 1352-2310 Institutional support: RVO:67179843 Keywords : Ozone fluxes * Stomatal conductance models * GPP * Mediterranean forest Subject RIV: EH - Ecology, Behaviour Impact factor: 3.062, year: 2013

  4. Chromatin modification of Notch targets in olfactory receptor neuron diversification

    Czech Academy of Sciences Publication Activity Database

    Endo, K.; Karim, M. R.; Taniguchi, H.; Krejčí, Alena; Kinameri, E.; Siebert, M.; Ito, K.; Bray, S. J.; Moore, A. W.

    2012-01-01

    Roč. 15, č. 2 (2012), s. 224-233. ISSN 1097-6256 Institutional research plan: CEZ:AV0Z50070508 Keywords : neuron diversification Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 15.251, year: 2012

  5. Particle–particle collisions in the Lagrangian modelling of saltating grains By ROBERT J. BIALIK, Journal of Hydraulic Research, Vol. 49, No. 1 (2011), pp. 23–31

    Czech Academy of Sciences Publication Activity Database

    Lukerchenko, Nikolay

    2012-01-01

    Roč. 50, č. 2 (2012), s. 251-252. ISSN 0022-1686 Institutional research plan: CEZ:AV0Z20600510 Keywords : saltation * spherical particle * particle-particle collision * numerical model Subject RIV: BK - Fluid Dynamics

  6. Steady-state isotope power for deep-space explorations

    International Nuclear Information System (INIS)

    Radioisotope power is essential for space explorations. While space missions lasting 25 years require a sustained power-to-weight ratio hardly attainable for current spacecraft designs and expected mission functions. In this paper, the authors report the results of an analysis that suggests that the isotope pairs 251Cf and 252Cf, operating in a tandem reaction network, are most appropriate fuels even for space missions well in excess of 25 years. The isotopes 251Cf and 252Cf possess a rare and highly complementary set of nuclear properties: fission and neutron capture cross sections for 251Cf are exceptionally high, 4,880 and 2,700 b, respectively, providing therefore both fission energy and breeding of 252Cf, this bred 252Cf isotope possesses decay channels for both alpha decay and spontaneous fission. As a consequence of these properties, injected 251Cf can sustain a fission chain by autocatalysis as well as by spontaneous fission

  7. Intrinsic defects, nonstoichiometry and aliovalent doping of A.sup.2+./sup. B.sup.4+./sup.O.sub.3./sub. perovskite scintillators

    Czech Academy of Sciences Publication Activity Database

    Casillas-Trujillo, L.; Andersson, D.A.; Dorado, B.; Nikl, Martin; Sickafus, K.E.; McClellan, K.J.; Stanek, C.R.

    2014-01-01

    Roč. 251, č. 11 (2014), s. 2279-2286. ISSN 0370-1972 Institutional support: RVO:68378271 Keywords : atomistic simulation * defects * nonstoichiometry * perovskites * scintillators Subject RIV: BM - Solid Matter Physics ; Magnetism Impact factor: 1.489, year: 2014

  8. Cytochemical and ultrastructural aspects of aquatic carnivorous plant turions

    Czech Academy of Sciences Publication Activity Database

    Plachno, B.J.; Adamec, Lubomír; Kozieradzka-Kiszkurno, M.; Świątek, P.; Kamińska, I.

    2014-01-01

    Roč. 251, č. 6 (2014), s. 1449-1454. ISSN 0033-183X Institutional support: RVO:67985939 Keywords : aquatic carnivorous plants * winter buds * storage functions Subject RIV: EF - Botanics Impact factor: 2.651, year: 2014

  9. Kulturní pluralismus a interkulturalismus. Na příkladu národností v České republice (Československu)

    Czech Academy of Sciences Publication Activity Database

    Nosková, Helena

    Praha: FHS UK, 2012 - (Bittnerová, D.; Moravcová, M.), s. 233-251. (Agora. 9). ISBN 978-80-87398-28-9 Institutional support: RVO:68378114 Keywords : national minorities * contemporary history * cultural pluralism Subject RIV: AB - History

  10. The Chlamydomonas genome reveals the evolution of key animal and plant functions

    Czech Academy of Sciences Publication Activity Database

    Merchant, S.S.; Prochnik, S. E.; Bišová, Kateřina

    2007-01-01

    Roč. 318, - (2007), s. 245-251. ISSN 0036-8075 Institutional research plan: CEZ:AV0Z50200510 Keywords : chlamydomonas reinhardtii * alga * eukaryotic cell Subject RIV: EE - Microbiology, Virology Impact factor: 26.372, year: 2007

  11. Motivational Components of Client Reactance.

    Science.gov (United States)

    Dowd, E. Thomas; Wallbrown, Fred

    1993-01-01

    Attempted to determine motivational personality characteristics associated with psychological reactance. Undergraduate students (n=251) took Therapeutic Reactance Scale, Questionnaire for Measuring Psychological Reactance, and Personality Research Form. Results showed personality patterns of the psychologically reactant person as defensive,…

  12. The Pursuit of Ecotopia: Lessons from Indigenous and Traditional Societies for the Human Ecology of Our Modern World

    OpenAIRE

    Steve Wolverton

    2010-01-01

    Review of The Pursuit of Ecotopia: Lessons from Indigenous and Traditional Societies for the Human Ecology of Our Modern World. Eugene N. Anderson. 2010. Praeger Publishers, Santa Barbara. Pp. 251. $44.95 (Hardcover). ISBN 0313381305.

  13. Změny Římského statutu Mezinárodního trestního soudu

    Czech Academy of Sciences Publication Activity Database

    Šturma, Pavel

    2010-01-01

    Roč. 9, č. 8 (2010), s. 245-251. ISSN 1213-5313 Institutional research plan: CEZ:AV0Z70680506 Keywords : public international law * International Criminal Court * crime of agression Subject RIV: AG - Legal Sciences

  14. Německý Mácha, Óda na radost a krize evropské vize

    Czech Academy of Sciences Publication Activity Database

    Pokorný, Martin Z.

    2013-01-01

    Roč. 31, 97-98 (2013), s. 244-251. ISSN 0862-7045 Institutional support: RVO:67985955 Keywords : K. H. Mácha * F. Schiller * A. Mozart * Enlightenment * romanticism Subject RIV: AA - Philosophy ; Religion

  15. QTL Information Table: 369 [Q-TARO

    Lifescience Database Archive (English)

    Full Text Available qET-6 Physiological trait Eating quality Endosperm translucency RFLP C)Interval BIL...tative trait loci underlying appearance quality of rice grains (Oryza sativa L.). Yi Chuan Xue Bao 30, 251-259. ...

  16. QTL Information Table: 368 [Q-TARO

    Lifescience Database Archive (English)

    Full Text Available qPGWC-6b Physiological trait Eating quality Percentages of grains with chalkiness R...3). Mapping quantitative trait loci underlying appearance quality of rice grains (Oryza sativa L.). Yi Chuan Xue Bao 30, 251-259. ...

  17. Forced flow and solidification over a moving substrate

    Czech Academy of Sciences Publication Activity Database

    Kyselica, Juraj; Guba, P.

    2016-01-01

    Roč. 40, č. 1 (2016), s. 31-40. ISSN 0307-904X Institutional support: RVO:67985530 Keywords : binary alloy * solidification * moving substrate Subject RIV: DE - Earth Magnetism, Geodesy, Geography Impact factor: 2.251, year: 2014

  18. Effects of Aedes aegypti salivary components on dendritic cell and lymphocyte biology

    Czech Academy of Sciences Publication Activity Database

    Bizzarro, B.; Barros, M.S.; Maciel, C.; Gueroni, D.I.; Lino, C.N.; Campopiano, J.; Kotsyfakis, Michalis; Amarante-Mendes, G.P.; Calvo, E.; Capurro, M.L.; Sa-Nunes, A.

    2013-01-01

    Roč. 6, NOV 2013 (2013), s. 329. ISSN 1756-3305 Institutional support: RVO:60077344 Keywords : dendritic cells * T-cells * Aedes aegypti * saliva * apoptosis Subject RIV: EC - Immunology Impact factor: 3.251, year: 2013

  19. Thermal Manifestations and Nanoindentation of Bone Cements for Orthopaedic Surgery

    Czech Academy of Sciences Publication Activity Database

    Hloch, Sergej; Monka, P.; Hvizdoš, P.; Jakubéczyová, D.; Kozak, D.; Čolič, K.; Kloc, J.; Magurová, D.

    2013-01-01

    Roč. 17, č. 1 (2013), s. 251-258. ISSN 0354-9836 Institutional support: RVO:68145535 Keywords : bone cement * exothermic behaviour * nanoindentation * porosity * osteonecrosis Subject RIV: FJ - Surgery incl. Transplants Impact factor: 0.962, year: 2013

  20. Folic Acid

    Medline Plus

    Full Text Available ... birth and infant mortality. Solving premature birth Stanford University Prematurity Research Center Featured articles Accomplishments and lessons ... sickness 2:50 Prematurity Research Center at Washington University in St. Louis - CERVETTO Family 2:51 Inducing ...

  1. Gestational Diabetes

    Medline Plus

    Full Text Available ... birth and infant mortality. Solving premature birth Stanford University Prematurity Research Center Featured articles Accomplishments and lessons ... sickness 2:50 Prematurity Research Center at Washington University in St. Louis - CERVETTO Family 2:51 Inducing ...

  2. Healthy Choices: What to Eat during Pregnancy

    Medline Plus

    Full Text Available ... birth and infant mortality. Solving premature birth Stanford University Prematurity Research Center Featured articles Accomplishments and lessons ... sickness 2:50 Prematurity Research Center at Washington University in St. Louis - CERVETTO Family 2:51 Inducing ...

  3. Gestational Hypertension and Preeclampsia

    Medline Plus

    Full Text Available ... birth and infant mortality. Solving premature birth Stanford University Prematurity Research Center Featured articles Accomplishments and lessons ... sickness 2:50 Prematurity Research Center at Washington University in St. Louis - CERVETTO Family 2:51 Inducing ...

  4. Cesarean Section: Recovering After Surgery

    Medline Plus

    Full Text Available ... birth and infant mortality. Solving premature birth Stanford University Prematurity Research Center Featured articles Accomplishments and lessons ... sickness 2:50 Prematurity Research Center at Washington University in St. Louis - CERVETTO Family 2:51 Inducing ...

  5. Cesarean Section: The Operation

    Medline Plus

    Full Text Available ... birth and infant mortality. Solving premature birth Stanford University Prematurity Research Center Featured articles Accomplishments and lessons ... sickness 2:50 Prematurity Research Center at Washington University in St. Louis - CERVETTO Family 2:51 Inducing ...

  6. Alcohol and Pregnancy

    Medline Plus

    Full Text Available ... birth and infant mortality. Solving premature birth Stanford University Prematurity Research Center Featured articles Accomplishments and lessons ... sickness 2:50 Prematurity Research Center at Washington University in St. Louis - CERVETTO Family 2:51 Inducing ...

  7. Labor and Delivery: At the Hospital

    Medline Plus

    Full Text Available ... birth and infant mortality. Solving premature birth Stanford University Prematurity Research Center Featured articles Accomplishments and lessons ... sickness 2:50 Prematurity Research Center at Washington University in St. Louis - CERVETTO Family 2:51 Inducing ...

  8. At Least 39 Weeks

    Medline Plus

    Full Text Available ... birth and infant mortality. Solving premature birth Stanford University Prematurity Research Center Featured articles Accomplishments and lessons ... sickness 2:50 Prematurity Research Center at Washington University in St. Louis - CERVETTO Family 2:51 Inducing ...

  9. Exercise during Pregnancy

    Medline Plus

    Full Text Available ... birth and infant mortality. Solving premature birth Stanford University Prematurity Research Center Featured articles Accomplishments and lessons ... sickness 2:50 Prematurity Research Center at Washington University in St. Louis - CERVETTO Family 2:51 Inducing ...

  10. 9 CFR 206.3 - Monthly report.

    Science.gov (United States)

    2010-01-01

    ... STOCKYARDS PROGRAMS), DEPARTMENT OF AGRICULTURE SWINE CONTRACT LIBRARY § 206.3 Monthly report. (a) Do I need... accordance with section 251 of the Agricultural Marketing Act (7 U.S.C. 1636) and may be modified from...

  11. Adjustment to Spinal Cord Injury

    Science.gov (United States)

    ... may need to work to help with family finances. All of the changes that they face can ... 2000). Goal instability and adjustment to physical disability. Journal of Counseling Psychology, 47(2). 251-265. This ...

  12. Wide Output Range Power Processing Unit for Electric Propulsion Project

    Data.gov (United States)

    National Aeronautics and Space Administration — A power supply concept capable of operation over 25:1 and 64:1 impedance ranges at full power has been successfully demonstrated in our Phase I effort at...

  13. Hypocrisy or maturity? Culture and context differentiation

    Czech Academy of Sciences Publication Activity Database

    Matsumoto, D.; Yoo, S.H.; Fontaine, J.; Anguas-Wong, A.M.; Arriola, M.; Ataca, B.; Bond, M.H.; Boratav, H.B.; Breugelmans, S.M.; Cabecinhas, R.; Chae, J.; Chin, W.H.; Comunian, A.L.; DeGere, D.N.; Djunaidi, A.; Fok, H.K.; Friedlmeier, W.; Ghosh, A.; Glamcevski, M.; Granskaya, J.V.; Groenvynck, H.; Harb, C.; Haron, F.; Joshi, R.; Kakai, H.; Kashima, E.; Khan, W.; Kurman, J.; Kwantes, C.T.; Mahmud, S.H.; Mandaric, M.; Nizharadze, G.; Odusanya, J.O.T.; Ostrosky-Solis, F.; Palaniappan, A.K.; Papastylianou, D.; Poláčková Šolcová, Iva; Safdar, S.; Setiono, K.; Shigemasu, E.; Singelis, T.M.; Spiess, E.; Sterkowicz, S.; Sunar, D.; Szarota, P.; Vishnivetz, B.; Vohra, N.; Ward, C.; Wong, S.; Wu, R.; Zebian, S.; Zengeya, A.

    2009-01-01

    Roč. 23, č. 3 (2009), s. 251-264. ISSN 0890-2070 Institutional research plan: CEZ:AV0Z70250504 Keywords : personality - traits * individualism * consistency Subject RIV: AN - Psychology Impact factor: 2.597, year: 2009

  14. Planetary wave modulation of upward propagating tides and gravity waves as contribution to planetary wave type oscilations in the f2 region

    Czech Academy of Sciences Publication Activity Database

    Laštovička, Jan

    Didcot: CLRC Rutherford Appleton Laboratory, 1999 - (Vernon, A.), s. 192-197 [COST251 Workshop 'Impact of the Upper Atmosphere on Terrestrial and Earth-Space Communications' /4./. Madeira (PT), 22.03.1999-25.03.1999] R&D Projects: GA MŠk OC 251.10 Institutional research plan: CEZ:AV0Z3042911 Subject RIV: BL - Plasma and Gas Discharge Physics

  15. AcEST: DK951614 [AcEST

    Lifescience Database Archive (English)

    Full Text Available TST38A01NGRL0011_O01 678 Adiantum capillus-veneris mRNA. clone: TST38A01NGRL0011_O01. 5' end seq ... %) Frame = +3 Query: 72 VRREIKILRLFMHTHIIRLYEVIETETDIY FVMEYVNSGELFDHIVEKGRLSEDEARRFF 251 VRREIKILRLFMH HI ... IRLYEVIET TDIY ... VMEYVNSGELFD+IVEKGRL EDEAR FF Sbjct: 87 VRREIKILRL ... FMHPHIIRLYEVIETPTDIY LVMEYVNSGELFDYIVEKGRLQEDEARNFF 146 Query: 252 QQVLA ... %) Frame = +3 Query: 72 VRREIKILRLFMHTHIIRLYEVIETETDIY FVMEYVNSGELFDHIVEKGRLSEDEARRFF 251 VRREIKILRLFMH HI ...

  16. Drug: D05017 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available ics Agents against Amebiasis and other antiprotozoals Nitroimidazole derivatives Metronidazole [ATC:A01AB17 ...D05017 Drug Metronidazole phosphate (USAN) C6H10N3O6P 251.0307 251.1339 D05017.gif Antibacterial; Antiprotoz...oal ATC code: A01AB17 D06BX01 G01AF01 J01XD01 P01AB01 Anatomical Therapeutic Chemic

  17. CB1 cannabinoid receptor antagonism promotes remodeling and cannabinoid treatment prevents endothelial dysfunction and hypotension in rats with myocardial infarction

    OpenAIRE

    Wagner, Jens A.; Hu, Kai; Karcher, Jan; Bauersachs, Johann; Schäfer, Andreas; Laser, Martin; Han, Hong; Ertl, Georg

    2003-01-01

    To study the long-term effects of altered cannabinoid receptor activity on myocardial and vascular function, Wistar rats were treated with the selective CB1 antagonist AM-251 (0.5 mg kg−1 d−1), the potent synthetic cannabinoid HU-210 (50 μg kg−1 d−1) or vehicle for 12 weeks after coronary artery ligation or sham operation.AM-251 further reduced the pressure-generating capacity, shifted the pressure volume curve to the right (P

  18. AcEST: DK954608 [AcEST

    Lifescience Database Archive (English)

    Full Text Available TST39A01NGRL0020_O08 676 Adiantum capillus-veneris mRNA. clone: TST39A01NGRL0020_O08. 5' end seq ... e incorporator OS=Dictyostel... 68 4e-11 sp|Q12116|TMS 1_YEAST Membrane protein TMS 1 OS=Saccharomyces cere ... za sativa subs... 251 4e-65 tr|Q10IH0|Q10IH0_ORYSJ TMS ... membrane family protein, putative, ex... 251 4e-65 ...

  19. Enhancing the thermostability of a cold-active lipase from Penicillium cyclopium by in silico design of a disulfide bridge.

    Science.gov (United States)

    Tan, Zhongbiao; Li, Jianfang; Wu, Minchen; Wang, Junqing

    2014-08-01

    Cysteine mutants of a cold-active lipase (PcLipI) from Penicillium cyclopium were designed by the software Disulfide by Design Ver. 1.20 in an effort to improve enzyme thermostability by addition of a disulfide bridge. Those mutants predicted by molecular dynamics simulation to have better thermostability than the wild type were first expressed in Escherichia coli BL21(DE3) and then, for further investigation, in Pichia pastoris GS115. By replacing Val248 and Thr251 with cysteines to create a disulfide bridge, the recombinant lipases reE-PcLipV248C-T251C (expressed in E. coli) and reP-PcLipV248C-T251C (expressed in P. pastoris) were obtained. Both had enhanced thermostability with half-lives at 35 °C about 4.5- and 12.8-fold longer than that of the parent PcLipI expressed in E. coli and P. pastoris, respectively. The temperature optima of reE-PcLipV248C-T251C and reP-PcLipV248C-T251C were 35 and 30 °C, which were each 5 °C higher than those of the parent PcLipI expressed in E. coli and P. pastoris. The K ms of reE-PcLipV248C-T251C and reP-PcLipV248C-T251C toward tributyrin were 53.2 and 39.5 mM, while their V maxs were 1,460 and 3,800 U/mg, respectively. PcLipV248C-T251C had better thermostability and catalytic efficiency than the other mutants and the parent PcLipI. PMID:24867629

  20. Dicty_cDB: VHM290 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available slsvxllnpsmsllvtl--- ---tsirilrfhr*ftfpqcrssfptmcxxslgfnwccqqr*...s MGC108369 prote... 252 1e-65 DQ357217_1( DQ357217 |pid:none) Leishmania braziliensis elongation... 251... 2e-65 AM494972_26( AM494972 |pid:none) Leishmania braziliensis chromosom... 251 2e-65 CT005272_19( CT005272 |pid:none) Leishmania...fnqsyrfp rsr*fqfrsnccppcy*wcfsryrlrrrclrtnrncitssrr*tyqtslirqqsrsflir...flvi*dichvvlmnksvvsplnlhlyhftskrqrktnyqlvvlhtnf*silsipq vtlisvqk*llpsvllmvl*slsiaskvfaykpklyyvkpslnvsnqsysstksivsy*n sn*tlkkli

  1. Book Review: Training the Party: Party Adaptation and Elite Training in Reform-era China

    DEFF Research Database (Denmark)

    Brødsgaard, Kjeld Erik

    2016-01-01

    Review of: Training the Party: Party Adaptation and Elite Training in Reform-era China. Charlotte P. Lee . Cambridge: Cambridge University Press, 2015. xii + 251 pp. $99.99. ISBN 978-1-107-09063-7......Review of: Training the Party: Party Adaptation and Elite Training in Reform-era China. Charlotte P. Lee . Cambridge: Cambridge University Press, 2015. xii + 251 pp. $99.99. ISBN 978-1-107-09063-7...

  2. ОСОБЕННОСТИ АССОЦИАЦИИ ПОЛИМОРФНЫХ МАРКЕРОВ -251 Т>А ГЕНА IL-8 И ПОЛИМОРФИЗМ ГЕНОВ HELICOBACTER PYLORI У КОРЕННЫХ И ПРИШЛЫХ ЖИТЕЛЕЙ ХАКАСИИ

    OpenAIRE

    Агеева, Елизавета; Штыгашева, Ольга

    2012-01-01

    Исследовали полиморфизм гена интерлейкина IL-8 при Helicobacter pylori-ассоциированных заболеваниях: язвенной болезни и хроническом, гастрите у коренных и пришлых жителей Хакасии методом рестрикционного анализа. Распространенность субтипов VacA+ и. CagA+ Helicobacter pylori проводили методом, полимеразной цепной реакции. Выявлены, наиболее распространенные аллельные варианты генов цитокинов среди, хакасов. Обсуждается, целесообразность определения популяционных рисковых и. протективных геноти...

  3. Synthetic Ligands of Cannabinoid Receptors Affect Dauer Formation in the Nematode Caenorhabditis elegans.

    Science.gov (United States)

    Reis Rodrigues, Pedro; Kaul, Tiffany K; Ho, Jo-Hao; Lucanic, Mark; Burkewitz, Kristopher; Mair, William B; Held, Jason M; Bohn, Laura M; Gill, Matthew S

    2016-01-01

    Under adverse environmental conditions the nematode Caenorhabditis elegans can enter an alternate developmental stage called the dauer larva. To identify lipophilic signaling molecules that influence this process, we screened a library of bioactive lipids and found that AM251, an antagonist of the human cannabinoid (CB) receptor, suppresses dauer entry in daf-2 insulin receptor mutants. AM251 acted synergistically with glucose supplementation indicating that the metabolic status of the animal influenced the activity of this compound. Similarly, loss of function mutations in the energy-sensing AMP-activated kinase subunit, aak-2, enhanced the dauer-suppressing effects of AM251, while constitutive activation of aak-2 in neurons was sufficient to inhibit AM251 activity. Chemical epistasis experiments indicated that AM251 acts via G-protein signaling and requires the TGF-β ligand DAF-7, the insulin peptides DAF-28 and INS-6, and a functional ASI neuron to promote reproductive growth. AM251 also required the presence of the SER-5 serotonin receptor, but in vitro experiments suggest that this may not be via a direct interaction. Interestingly, we found that other antagonists of mammalian CB receptors also suppress dauer entry, while the nonselective CB receptor agonist, O-2545, not only inhibited the activity of AM251, but also was able to promote dauer entry when administered alone. Since worms do not have obvious orthologs of CB receptors, the effects of synthetic CBs on neuroendocrine signaling in C. elegans are likely to be mediated via another, as yet unknown, receptor mechanism. However, we cannot exclude the existence of a noncanonical CB receptor in C. elegans. PMID:27172180

  4. Phorbol 12-myristate 13-acetate induces protein kinase ceta-specific proliferative response in astrocytic tumor cells.

    Science.gov (United States)

    Hussaini, I M; Karns, L R; Vinton, G; Carpenter, J E; Redpath, G T; Sando, J J; VandenBerg, S R

    2000-07-21

    Protein kinase C (PKC) activation has been implicated in cellular proliferation in neoplastic astrocytes. The roles for specific PKC isozymes in regulating this glial response, however, are not well understood. The aim of this study was to characterize the expression of PKC isozymes and the role of PKC-eta expression in regulating cellular proliferation in two well characterized astrocytic tumor cell lines (U-1242 MG and U-251 MG) with different properties of growth in cell culture. Both cell lines expressed an array of conventional (alpha, betaI, betaII, and gamma) and novel (theta and epsilon) PKC isozymes that can be activated by phorbol myristate acetate (PMA). Another novel PKC isozyme, PKC-eta, was only expressed by U-251 MG cells. In contrast, PKC-delta was readily detected in U-1242 MG cells but was present only at low levels in U-251 MG cells. PMA (100 nm) treatment for 24 h increased cell proliferation by over 2-fold in the U-251 MG cells, whereas it decreased the mitogenic response in the U-1242 MG cells by over 90%. When PKC-eta was stably transfected into U-1242 MG cells, PMA increased cell proliferation by 2.2-fold, similar to the response of U-251 MG cells. The cell proliferation induced by PMA in both the U-251 MG and U-1242-PKC-eta cells was blocked by the PKC inhibitor bisindolylmaleimide (0.5 micrometer) and the MEK inhibitor, PD 98059 (50 micrometer). Transient transfection of wild type U-251 with PKC-eta antisense oligonucleotide (1 micrometer) also blocked the PMA-induced increase in [(3)H]thymidine incorporation. The data demonstrate that two glioblastoma lines, with functionally distinct proliferative responses to PMA, express different novel PKC isozymes and that the differential expression of PKC-eta plays a determining role in the different proliferative capacity. PMID:10806212

  5. MGMT反义RNA对人脑胶质瘤耐药性逆转的研究%Reveral of drug resistance of human glioma by MGMT antisense RNA

    Institute of Scientific and Technical Information of China (English)

    金澎; 张庆林; 刘福生; 王忠诚; 魏林; 王成伟; 孔建新

    2003-01-01

    目的利用反义技术逆转胶质瘤的耐药性.方法模拟临床亚硝基脲类药物的用药程序,在体外建立由DNA修复蛋白O6-甲基鸟嘌呤-DNA甲基转移酶(MGMT)介导的人脑胶质瘤耐药细胞系U251/BCNU,并进行细胞耐药性检测和细胞MGMTmRNA表达的分析;观察MGMT反义RNA对U251/BCNU细胞MGMT表达的调节作用及对U251/BCNU细胞BCNU敏感性的影响.结果经过反复总共5次的用药过程,首次在体外建立了对BCNU具有稳定抗性的U251/BCNU,其对BCNU的耐受程度约为U251的17倍;RT-PCR结果显示,U251/BCNU 细胞有MGMTmRNA的表达.MGMT反义RNA 表达载体pLaMT5SN和pLaMTSN能够在一定程度上降低U251/BCNU细胞中MGMT mRNA的表达水平,提高其对BCNU的敏感性.结论 MGMT反义RNA能够在一定程度上抑制MGMT的表达水平,提高肿瘤细胞对亚硝基脲类药物的敏感性.

  6. Minor Actinide Burning in Thermal Reactors. A Report by the Working Party on Scientific Issues of Reactor Systems

    International Nuclear Information System (INIS)

    The actinides (or actinoids) are those elements in the periodic table from actinium upwards. Uranium (U) and plutonium (Pu) are two of the principal elements in nuclear fuel that could be classed as major actinides. The minor actinides are normally taken to be the triad of neptunium (Np), americium (Am) and curium (Cm). The combined masses of the remaining actinides (i.e. actinium, thorium, protactinium, berkelium, californium, einsteinium and fermium) are small enough to be regarded as very minor trace contaminants in nuclear fuel. Those elements above uranium in the periodic table are known collectively as the transuranics (TRUs). The operation of a nuclear reactor produces large quantities of irradiated fuel (sometimes referred to as spent fuel), which is either stored prior to eventual deep geological disposal or reprocessed to enable actinide recycling. A modern light water reactor (LWR) of 1 GWe capacity will typically discharge about 20-25 tonnes of irradiated fuel per year of operation. About 93-94% of the mass of uranium oxide irradiated fuel is comprised of uranium (mostly 238U), with about 4-5% fission products and ∼1% plutonium. About 0.1-0.2% of the mass is comprised of neptunium, americium and curium. These latter elements accumulate in nuclear fuel because of neutron captures, and they contribute significantly to decay heat loading and neutron output, as well as to the overall radio-toxic hazard of spent fuel. Although the total minor actinide mass is relatively small - approximately 20-25 kg per year from a 1 GWe LWR - it has a disproportionate impact on spent fuel disposal, and thus the longstanding interest in transmuting these actinides either by fission (to fission products) or neutron capture in order to reduce their impact on the back end of the fuel cycle. The combined masses of the trace actinides actinium, thorium, protactinium, berkelium and californium in irradiated LWR fuel are only about 2 parts per billion, which is far too low for

  7. Radiological safety considerations in the design and operation of the Oak Ridge National Laboratory Transuranium Research Laboratory (TRL)

    International Nuclear Information System (INIS)

    The Transuranium Research Laboratory (TRL) is the central facility at Oak Ridge National Laboratory (ORNL) for chemical and physical research involving transuranium elements. Transuranium Research Laboratory investigations are about equally divided between studies of inorganic and structural chemistry of the heavy elements and nuclear structure and properties of their isotopes. Elements studied include neptunium, plutonium, americium, curium, berkelium, californium, and einsteinium, each in microgram-to-gram quantities depending upon availability and experimental requirements. This paper describes an eight-step safety procedure that is followed in planning and approving individual research projects. This procedure should provide an ''optimum margin of safety'' and should permit the accomplishment of successful research. Steps in the procedure are as follows: (1) Evaluate the external and internal exposure radioactivity hazards. (2) Establish any concurrent conventional industrial safety risks that may be encountered in the operation. (3) Consider, in the proper perspective, the potential consequences of possible interactions of radioactivity risks with associated industrial safety risks and problems, applying the concepts of credibility and probability. (4) Design equipment, develop operating procedures, and ensure the proficiency of operating personnel to provide necessary safeguards to accomplish programme objectives with minimum risks and minimum operational constraints. (5) Ensure that necessary safeguards are, in fact, incorporated in equipment and procedures before actual work is begun. (6) Ensure that appropriate monitoring instrumentation, surveillance, and other necessary protective technical and craft support are available and are used during operation. (7) Make certain that appropriate measures exist to manage credible radiation accidents and other emergencies properly. (8) Inform others, in sufficient detail, of the success or deficiency of design

  8. Radiochemical studies of neutron deficient actinide isotopes

    International Nuclear Information System (INIS)

    The production of neutron deficient actinide isotopes in heavy ion reactions was studied using alpha, gamma, x-ray, and spontaneous fission detection systems. A new isotope of berkelium, 242Bk, was produced with a cross-section of approximately 10 μb in reactions of boron on uranium and nitrogen on thorium. It decays by electron capture with a half-life of 7.0 +- 1.3 minutes. The alpha-branching ratio for this isotope is less than 1% and the spontaneous fission ratio is less than 0.03%. Studies of (Heavy Ion, pxn) and (Heavy Ion, αxn) transfer reactions in comparison with (Heavy ion, xn) compound nucleus reactions revealed transfer reaction cross-sections equal to or greater than the compound nucleus yields. The data show that in some cases the yield of an isotope produced via a (H.I.,pxn) or (H.I.,αxn) reaction may be higher than its production via an xn compound nucleus reaction. These results have dire consequences for proponents of the ''Z1 + Z2 = Z/sub 1+2/'' philosophy. It is no longer acceptable to assume that (H.I.,pxn) and (H.I.,αxn) product yields are of no consequence when studying compound nucleus reactions. No evidence for spontaneous fission decay of 228Pu, 230Pu, 232Cm, or 238Cf was observed indicating that strictly empirical extrapolations of spontaneous fission half-life data is inadequate for predictions of half-lives for unknown neutron deficient actinide isotopes

  9. Transuranium Processing Plant semiannual report of production, status, and plans for period ending December 31, 1975

    Energy Technology Data Exchange (ETDEWEB)

    King, L.J.; Bigelow, J.E.; Collins, E.D.

    1976-10-01

    Between July 1, 1975, and December 31, 1975, maintenance was conducted at TRU for a period of three months, 295 g of curium oxide (enough for approximately 26 HFIR targets) were prepared, 100 mg of high-purity /sup 248/Cm, were separated from /sup 252/Cf that had been purified during earlier periods, 11 HFIR targets were fabricated, and 28 product shipments were made. No changes were made in the chemical processing flowsheets normally used at TRU during this report period. However, three equipment racks were replaced (with two new racks) during this time. In Cubicle 6, the equipment replaced was that used to decontaminate the transplutonium elements from rare earth fission products and to separate curium from the heavier elements by means of the LiCl-based anion-exchange process. In Cubicle 5, the equipment used to separate the transcurium elements by high-pressure ion exchange and to purify berkelium by batch solvent extraction was replaced. Two neutron sources were fabricated, bringing the total fabricated to 79. One source that had been used in a completed project was returned to the TRU inventory and is available for reissue. Three sources, for which no further use was foreseen, were processed to isolate and recover the ingrown /sup 248/Cm and the residual /sup 252/Cf. Eight pellets, each containing 100 ..mu..g of high-purity /sup 248/Cm were prepared for irradiation in HFIR to study the production of /sup 250/Cm. The values currently being used for transuranium element decay data and for cross-section data in planning irradiation-processing cycles, calculating production forecasts, and assaying products are tabulated.

  10. Radiochemical studies of neutron deficient actinide isotopes

    Energy Technology Data Exchange (ETDEWEB)

    Williams, K.E.

    1978-04-01

    The production of neutron deficient actinide isotopes in heavy ion reactions was studied using alpha, gamma, x-ray, and spontaneous fission detection systems. A new isotope of berkelium, /sup 242/Bk, was produced with a cross-section of approximately 10 ..mu..b in reactions of boron on uranium and nitrogen on thorium. It decays by electron capture with a half-life of 7.0 +- 1.3 minutes. The alpha-branching ratio for this isotope is less than 1% and the spontaneous fission ratio is less than 0.03%. Studies of (Heavy Ion, pxn) and (Heavy Ion, ..cap alpha..xn) transfer reactions in comparison with (Heavy ion, xn) compound nucleus reactions revealed transfer reaction cross-sections equal to or greater than the compound nucleus yields. The data show that in some cases the yield of an isotope produced via a (H.I.,pxn) or (H.I.,..cap alpha..xn) reaction may be higher than its production via an xn compound nucleus reaction. These results have dire consequences for proponents of the ''Z/sub 1/ + Z/sub 2/ = Z/sub 1+2/'' philosophy. It is no longer acceptable to assume that (H.I.,pxn) and (H.I.,..cap alpha..xn) product yields are of no consequence when studying compound nucleus reactions. No evidence for spontaneous fission decay of /sup 228/Pu, /sup 230/Pu, /sup 232/Cm, or /sup 238/Cf was observed indicating that strictly empirical extrapolations of spontaneous fission half-life data is inadequate for predictions of half-lives for unknown neutron deficient actinide isotopes.

  11. Paul Scherrer Institute Scientific Report 1999. Volume I: Particles and Matter

    Energy Technology Data Exchange (ETDEWEB)

    Gobrecht, J.; Gaeggeler, H.; Herlach, D.; Junker, K.; Kettle, P.-R.; Kubik, P.; Zehnder, A. [eds.

    2000-07-01

    lthough originally planned for fundamental research in nuclear physics, the particle beams of pions, muons, protons and neutrons are now used in a large variety of disciplines in both natural science and medicine. The beams at PSI have the world's highest intensities and therefore allow certain experiments to be performed, which would not be possible elsewhere. The highlight of research this year was the first-ever determination of the chemical properties of the superheavy element {sup 107} Bohrium. This was undertaken, by an international team led by H. Gaeggeler of PSI's Laboratory for Radiochemistry. Bohrium was produced by bombarding a Berkelium target with Neon ions from the Injector I cyclotron and six atoms were detected after having passed through an online gas chromatography device. At the Laboratory for Particle Physics the focus has shifted from nuclear physics to elementary particle physics with about a fifty-fifty split between investigations of rare processes or particle decays using the high intensity muon, pion and recently also polarized neutron beams of PSI, and research at the highest energy frontier at CERN (Geneva) and DESY (Hamburg). Important space instrumentation has been contributed by the Laboratory for Astrophysics to the European Space Agency and NASA satellite programmes. The Laboratory for Micro and Nanotechnology continued to focus on research into molecular nanotechnology and SiGeC nanostructures, the latter with the aim of producing silicon based optoelectronics. Progress in 1999 in these topical areas is described in this report. A list of scientific publications in 1999 is also provided.

  12. Conditioned Media from Human Adipose Tissue-Derived Mesenchymal Stem Cells and Umbilical Cord-Derived Mesenchymal Stem Cells Efficiently Induced the Apoptosis and Differentiation in Human Glioma Cell Lines In Vitro

    Directory of Open Access Journals (Sweden)

    Chao Yang

    2014-01-01

    Full Text Available Human mesenchymal stem cells (MSCs have an intrinsic property for homing towards tumor sites and can be used as tumor-tropic vectors for tumor therapy. But very limited studies investigated the antitumor properties of MSCs themselves. In this study we investigated the antiglioma properties of two easily accessible MSCs, namely, human adipose tissue-derived mesenchymal stem cells (ASCs and umbilical cord-derived mesenchymal stem cells (UC-MSCs. We found (1 MSC conditioned media can significantly inhibit the growth of human U251 glioma cell line; (2 MSC conditioned media can significantly induce apoptosis in human U251 cell line; (3 real-time PCR experiments showed significant upregulation of apoptotic genes of both caspase-3 and caspase-9 and significant downregulation of antiapoptotic genes such as survivin and XIAP after MSC conditioned media induction in U 251 cells; (4 furthermore, MSCs conditioned media culture induced rapid and complete differentiation in U251 cells. These results indicate MSCs can efficiently induce both apoptosis and differentiation in U251 human glioma cell line. Whereas UC-MSCs are more efficient for apoptosis induction than ASCs, their capability of differentiation induction is not distinguishable from each other. Our findings suggest MSCs themselves have favorable antitumor characteristics and should be further explored in future glioma therapy.

  13. Inhibition of Glioblastoma Cell Growth In Vitro and In Vivo by Brucine, a Component of Chinese Medicine.

    Science.gov (United States)

    Ruijun, Wang; Wenbin, Meng; Yumin, Wang; Ruijian, Zhang; Puweizhong, Huang; Yulin, Li

    2014-01-01

    Glioblastoma multiforme (GBM) is one of the most common glial cell tumors and has drawn more and more attention in the clinic in recent years. Brucine has been reported to significantly suppress gastric cancer, lung cancer, and prostate cancer growth in vivo by inducing cell apoptosis. Here, the effects of brucine on U251 human glioma cell growth were investigated in vitro by cell proliferation assay, FACs, and qPCR in a xenograft tumor model. Treatment with brucine reduced the expression of BCL-2 and cyclooxygenase-2 (COX-2), while upregulated BAX expression in U251 human glioma cells resulted in reduced glioma cell survival rate and inhibited the growth of xenograft tumors. We concluded that brucine has a suppressive effect on U251 human glioma cells in vitro and in vivo, which could help in understanding the role of brucine in glioma cells and guiding drug use in the clinic. PMID:26629939

  14. Chryseobacterium chengduensis sp. nov. isolated from the air of captive giant panda enclosures in Chengdu, China.

    Science.gov (United States)

    Wen, Cai-Fang; Xi, Li-Xin; Zhao, Shan; Hao, Zhong-Xiang; Luo, Lu; Liao, Hong; Chen, Zhen-Rong; She, Rong; Han, Guo-Quan; Cao, San-Jie; Wu, Rui; Yan, Qi-Gui; Hou, Rong

    2016-08-01

    A Gram-negative, aerobic, non-motile, rod-shaped bacterial strain, designated 25-1(T), was isolated from the air inside giant panda enclosures at the Chengdu Research Base of Giant Panda Breeding, China. Strain 25-1(T) grew optimally at pH 7.0-8.0, at 28-30 °C and in the presence of NaCl concentrations from 0.0% to 0.5 %. 16S rRNA gene sequence analysis indicated that strain 25-1(T) belongs to the genus Chryseobacterium within the family Flavobacteriaceae and is related most closely to C. carnis G81(T) (96.4% similarity), C. lathyri RBA2-6(T) (95.8% similarity), and C. zeae JM1085(T) (95.8% similarity). Its genomic DNA G+C molar composition was 36.2%. The major cellular fatty acids were iso-C15:0 (44.0%), iso-C17:0 3OH (19.8%) and C16:1 ω7c/16:1 ω6c (12.7%). The only isoprenoid quinone was menaquinone 6 (MK-6). The major polar lipids were phosphatidylethanolamine, two unidentified amino lipids and two unidentified lipids. The DNA-DNA relatedness between strain 25-1(T) and C. lathyri RBA2-6(T) was 38%. Phenotypic, genotypic, and phylogenetic characteristics indicated that strain 25-1(T) is a novel member of the genus Chryseobacterium, for which the name C. chengduensis sp. nov. is proposed. The type strain is 25-1(T) (CCTCC AB2015133(T)=DSM 100396(T)). PMID:27487806

  15. The Effect of Chemoradiotherapy with SRC Tyrosine Kinase Inhibitor, PP2 and Temozolomide on Malignant Glioma Cells In Vitro and In Vivo

    Science.gov (United States)

    Eom, Keun-Yong; Cho, Bong Jun; Choi, Eun Jung; Kim, Jin-Ho; Chie, Eui Kyu; Wu, Hong-Gyun; Kim, Il Han; Paek, Sun Ha; Kim, Jae-Sung; Kim, In Ah

    2016-01-01

    Purpose We investigated the effect of chemoradiotherapy with PP2 and temozolomide (TMZ) on malignant glioma cells using clonogenic assays and in vivo brain tumor model. Materials and Methods The effect of PP2 on radiosensitivity of U251 and T98G cells was investigated using clonogenic assays. The expression of E-cadherin, matrix metalloproteinases 2 (MMP2), Ephrin type-A receptor 2 (EphA2), and vascular endothelial growth factor (VEGF) was measured by Western blotting and an accumulation of γH2AX foci 6 hours after radiotherapy was measured after PP2 treatment. The effect of PP2 on migration, invasion, and vasculogenic mimicry formation (VMF) of U251 cells was evaluated. In an orthotopical brain tumor model with U251 cells, PP2 was injected intraperitoneally with or without oral TMZ before, during and after whole brain radiotherapy. Bioluminescence images were taken to visualize in vivo tumors and immunohistochemical staining of VEGF, CD31, EphA2, and hypoxia-inducible factor 1a was performed. Results PP2 increased radiosensitivity of U251 and T98G cells without decreasing survival of normal human astrocytes. Chemoradiotherapy with PP2 and TMZ resulted in increased accumulation of γH2AX foci. PP2 induced overexpression of E-cadherin and suppression of MMP2, VEGF, and EphA2. PP2 also compromised invasion, migration, and VMF of U251 cells. In brain tumors, chemoradiotherapy with PP2 and TMZ decreased tumor volume best, but not statistically significantly compared with chemoradiotherapy with TMZ. The expression of VEGF and CD31 was suppressed in PP2-treated tumors. Conclusion PP2 enhances radiosensitivity of malignant glioma cells and suppresses invasion and migration of U251 cells. Chemoradiotherapy with PP2 and TMZ resulted in non-significant tumor volume decrease. PMID:26044161

  16. Chryseobacterium chengduensis sp. nov. isolated from the air of captive giant panda enclosures in Chengdu, China* #

    Science.gov (United States)

    Wen, Cai-fang; Xi, Li-xin; Zhao, Shan; Hao, Zhong-xiang; Luo, Lu; Liao, Hong; Chen, Zhen-rong; She, Rong; Han, Guo-quan; Cao, San-jie; Wu, Rui; Yan, Qi-gui; Hou, Rong

    2016-01-01

    A Gram-negative, aerobic, non-motile, rod-shaped bacterial strain, designated 25-1T, was isolated from the air inside giant panda enclosures at the Chengdu Research Base of Giant Panda Breeding, China. Strain 25-1T grew optimally at pH 7.0–8.0, at 28–30 °C and in the presence of NaCl concentrations from 0.0% to 0.5 %. 16S rRNA gene sequence analysis indicated that strain 25-1T belongs to the genus Chryseobacterium within the family Flavobacteriaceae and is related most closely to C. carnis G81T (96.4% similarity), C. lathyri RBA2-6T (95.8% similarity), and C. zeae JM1085T (95.8% similarity). Its genomic DNA G+C molar composition was 36.2%. The major cellular fatty acids were iso-C15:0 (44.0%), iso-C17:0 3OH (19.8%) and C16:1 ω7c/16:1 ω6c (12.7%). The only isoprenoid quinone was menaquinone 6 (MK-6). The major polar lipids were phosphatidylethanolamine, two unidentified amino lipids and two unidentified lipids. The DNA–DNA relatedness between strain 25-1T and C. lathyri RBA2-6T was 38%. Phenotypic, genotypic, and phylogenetic characteristics indicated that strain 25-1T is a novel member of the genus Chryseobacterium, for which the name C. chengduensis sp. nov. is proposed. The type strain is 25-1T (CCTCC AB2015133T=DSM 100396T). PMID:27487806

  17. Over-expression of tetraspanin 8 in malignant glioma regulates tumor cell progression

    Energy Technology Data Exchange (ETDEWEB)

    Pan, Si-Jian [Department of Neurosurgery, Rui Jin Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200025 (China); Wu, Yue-Bing [Department of Internal Medicine Oncology, Hubei Cancer Hospital, Wuhan, Hubei 430079 (China); Cai, Shang [Department of Radiotherapy and Oncology, the Second Affiliated Hospital of Soochow University, Suzhou 21500 (China); Pan, Yi-Xin; Liu, Wei [Department of Stereotactic and Functional Neurosurgery, Rui Jin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025 (China); Bian, Liu-Guan [Department of Neurosurgery, Rui Jin Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200025 (China); Sun, Bomin [Department of Stereotactic and Functional Neurosurgery, Rui Jin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025 (China); Sun, Qing-Fang, E-mail: sunqingfang11@163.com [Department of Neurosurgery, Rui Jin Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200025 (China)

    2015-03-13

    Tumor cell invasion and proliferation remain the overwhelming causes of death for malignant glioma patients. To establish effective therapeutic methods, new targets implied in these processes have to be identified. Tetraspanin 8 (Tspn8) forms complexes with a large variety of trans-membrane and/or cytosolic proteins to regulate several important cellular functions. In the current study, we found that Tspn8 was over-expressed in multiple clinical malignant glioma tissues, and its expression level correlated with the grade of tumors. Tspn8 expression in malignant glioma cells (U251MG and U87MG lines) is important for cell proliferation and migration. siRNA-mediated knockdown of Tspn8 markedly reduced in vitro proliferation and migration of U251MG and U87MG cells. Meanwhile, Tspn8 silencing also increased the sensitivity of temozolomide (TMZ), and significantly increased U251MG or U87MG cell death and apoptosis by TMZ were achieved with Tspn8 knockdown. We observed that Tspn8 formed a complex with activated focal adhesion kinase (FAK) in both human malignant glioma tissues and in above glioma cells. This complexation appeared required for FAK activation, since Tspn8 knockdown inhibited FAK activation in U251MG and U87MG cells. These results provide evidence that Tspn8 contributes to the pathogenesis of glioblastoma probably by promoting proliferation, migration and TMZ-resistance of glioma cells. Therefore, targeting Tspn8 may provide a potential therapeutic intervention for malignant glioma. - Highlights: • Tspn8 is over-expressed in multiple clinical malignant glioma tissues. • Tspn8 expression is correlated with the grade of malignant gliomas. • Tspn8 knockdown suppresses U251MG/U87MG proliferation and in vitro migration. • Tspn8 knockdown significantly increases TMZ sensitivity in U251MG/U87MG cells. • Tspn8 forms a complex with FAK, required for FAK activation.

  18. Over-expression of tetraspanin 8 in malignant glioma regulates tumor cell progression

    International Nuclear Information System (INIS)

    Tumor cell invasion and proliferation remain the overwhelming causes of death for malignant glioma patients. To establish effective therapeutic methods, new targets implied in these processes have to be identified. Tetraspanin 8 (Tspn8) forms complexes with a large variety of trans-membrane and/or cytosolic proteins to regulate several important cellular functions. In the current study, we found that Tspn8 was over-expressed in multiple clinical malignant glioma tissues, and its expression level correlated with the grade of tumors. Tspn8 expression in malignant glioma cells (U251MG and U87MG lines) is important for cell proliferation and migration. siRNA-mediated knockdown of Tspn8 markedly reduced in vitro proliferation and migration of U251MG and U87MG cells. Meanwhile, Tspn8 silencing also increased the sensitivity of temozolomide (TMZ), and significantly increased U251MG or U87MG cell death and apoptosis by TMZ were achieved with Tspn8 knockdown. We observed that Tspn8 formed a complex with activated focal adhesion kinase (FAK) in both human malignant glioma tissues and in above glioma cells. This complexation appeared required for FAK activation, since Tspn8 knockdown inhibited FAK activation in U251MG and U87MG cells. These results provide evidence that Tspn8 contributes to the pathogenesis of glioblastoma probably by promoting proliferation, migration and TMZ-resistance of glioma cells. Therefore, targeting Tspn8 may provide a potential therapeutic intervention for malignant glioma. - Highlights: • Tspn8 is over-expressed in multiple clinical malignant glioma tissues. • Tspn8 expression is correlated with the grade of malignant gliomas. • Tspn8 knockdown suppresses U251MG/U87MG proliferation and in vitro migration. • Tspn8 knockdown significantly increases TMZ sensitivity in U251MG/U87MG cells. • Tspn8 forms a complex with FAK, required for FAK activation

  19. Neki aspekti izbora boje zuba za mobilne proteze    

    OpenAIRE

    JEROLIMOV, Vjekoslav; Carek, Vlado; Stipetić, Daniel

    1986-01-01

    Istraživanje je izvršeno ina osnovi podataka iz bolesničkih kartona i radnih naloga 935 pacijenata s parcijalnim (381 muškarac, 251 žena) i 365 pacijenta s totalnim protezma (118 muškaraca, 251 žena). Sv,e proteze izrađene su s umjetnim zubima iz aikrilata (dvoslojnim) tvornice Poligalansplastik (Volčja Draga). U obradi podatka poslužila je kvalitativna statistička metoda po Crameru, a značajnost razlika provjerena je X2 — testom. U parcijalnih proteza primjetna je veća učestalost pojedini...

  20. Nanostructured hybrid composites based on fullerene C60 and nickel - synthesis, structural properties and development

    Czech Academy of Sciences Publication Activity Database

    Vacík, Jiří; Lavrentiev, Vasyl; Vorlíček, Vladimír; Bačáková, Lucie; Narumi, K.

    Taipei : IAMS Academia Sinica, 2008. s. 251-251. ISBN N. [New Diamond and Nano Carbons /2./ - NDNC 2008. 26.05.2008-29.05.2008, Taipei] R&D Projects: GA ČR(CZ) GA204/06/0225; GA AV ČR(CZ) KAN400480701; GA AV ČR(CZ) KAN101120701 Institutional research plan: CEZ:AV0Z50110509; CEZ:AV0Z10480505; CEZ:AV0Z10100520 Keywords : spo2 * nickel * fullerene composite * spectroscopy Subject RIV: EI - Biotechnology ; Bionics

  1. Higgs hunt gets heavy

    CERN Document Server

    Reich, Eugenie Samuel

    2004-01-01

    The determination of the existence and mass of Higgs particle is discussed. The Higgs particle drags on other particles as if miring them in molasses, thereby endowing them with mass. An analysis indicates that the Higgs' most likely mass is 117 GeV, and it could weigh as much as 251 GeV. A Large Hadron Collider (LHC), developed by CERN, the European particle physics laboratory in Geneva, Switzerland, is scheduled to start up in 2007 and will hunt for the Higgs at energies up to 251 GeV and beyond. (Edited abstract).

  2. Synaptic action of anandamide and related substances in mammalian brain

    OpenAIRE

    Liao, Cheng Yong

    2007-01-01

    Anandamide and the synthetic cannabimimetic drugs AM 404 and WIN 55,212-2 were found to inhibit the binding of [3H]batrachotoxinin A 20--benzoate (BTX) to voltage-gated sodium channels (VGSCs) and also to depress VGSC-dependent release of GABA and L-glutamic acid. These effects occur independently of CB-1 receptor activation since they were not attenuated by AM251 at concentrations known to antagonize CB-1 receptors, although at higher concentrations AM251 inhibited VGSCs also. These ...

  3. AcEST: DK957364 [AcEST

    Lifescience Database Archive (English)

    Full Text Available rophytes Contig ID CL91Contig1 Sequence GAGGCCCGCACGCGCCGAGCTTCTGCTTCCAGAGATACTCGTAGACCTCCGTGCTTTTGG TGATACCCATGGATCCCTA...AGACTATCACCTGGTGGAGAAGCTCGCCCAGTTTGATAGAGAGA GGATTCCTGAACGAGTGGTACAT...4 PE=2 SV=1 251 1e-66 sp|P32290|CATA_PHAAU Catalase OS=Phaseolus aureus PE=2 SV=1 251 1e-66 sp|O48560|CATA3...2... 249 5e-66 sp|P49317|CATA3_NICPL Catalase isozyme 3 OS=Nicotiana plumbagini... 248 1e-65 sp|O24339|CATA..._IPOBA Catalase OS=Ipomoea batatas PE=2 SV=1 233 4e-61 sp|P48352|CATA3_CUCPE Catalase isozyme 3 OS=Cucurbi

  4. Molecular detection and genetic characterization of kobuviruses and astroviruses in asymptomatic local pigs in East Africa

    OpenAIRE

    Amimo, Joshua O.; Okoth, Edward; Junga, Joseph O.; Ogara, William O; Njahira, Moses N.; Wang, Qiuhong; Vlasova, Anastasia N.; Saif, Linda J; Djikeng, Appolinaire

    2013-01-01

    In this study, swine fecal specimens (n = 251) collected from nursing and weaned piglets raised under smallholder production systems were screened for the presence of kobuviruses by RT-PCR. Porcine kobuviruses were detected in 13.1 % (33/251) of the samples. We demonstrated that porcine kobuvirus infections exist in indigenous pigs in Kenya and Uganda and that the prevalence was higher in young piglets than older pigs: nursing piglets (15 %), post-weaning (3-month-old) pigs (17 %), 4-month-ol...

  5. Polymorphism of inflammatory genes and arsenic methylation capacity are associated with urothelial carcinoma

    International Nuclear Information System (INIS)

    Chronic exposure to arsenic can generate reactive oxidative species, which can induce certain proinflammatory cytokines such as tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6) and interleukin-8 (IL-8). TNF-α, IL-6 and IL-8 have been shown to be involved in the development and progression of various cancers, including bladder cancer. This study aimed to investigate the joint effect of the polymorphism of TNF-α − 308 G/A, IL-6 − 174 G/C, IL-8 − 251 T/A and urinary arsenic profiles on urothelial carcinoma (UC) risk. This study evaluated 300 pathologically-confirmed cases of UC and 594 cancer-free controls. Urinary arsenic species were detected using high-performance liquid chromatography-linked hydride generator and atomic absorption spectrometry. The polymorphism of TNF-α − 308 G/A, IL-6 − 174 G/C and IL-8 − 251 T/A was determined using polymerase chain reaction-restriction fragment length polymorphism. The joint effects on UC risk were estimated by odds ratios and 95% confidence intervals using unconditional logistic regression. We found that the TNF-α − 308 A/A and IL-8 − 251 T/T polymorphisms were significantly associated with UC. Moreover, significant dose–response joint effect of TNF-α − 308 A/A or IL-8 − 251 T/T genotypes and arsenic methylation indices were seen to affect UC risk. The present results also showed a significant increase in UC risk in subjects with the IL-8 − 251 T/T genotype for each SD increase in urinary total arsenic and MMA%. In contrast, a significant decrease in UC risk was found in subjects who carried the IL-8 − 251 T/T genotype for each SD increase in DMA%. - Highlights: • Joint effect of the TNF-α -308 A/A genotype and urinary total arsenic affected UC. • Joint effect of the IL-8 -251 T/T genotype and urinary total arsenic affected UC. • Urinary total arsenic level, TNF-α -308 A/A and IL-8 -251 T/T genotype affected UC

  6. Polymorphism of inflammatory genes and arsenic methylation capacity are associated with urothelial carcinoma

    Energy Technology Data Exchange (ETDEWEB)

    Wu, Chia-Chang [School of Public Health, College of Public Health and Nutrition, Taipei Medical University, Taipei, Taiwan (China); Department of Urology, Taipei Medical University—Shuang Ho Hospital, Taipei, Taiwan (China); Huang, Yung-Kai [School of Oral Hygiene, College of Oral Medicine, Taipei Medical University, Taipei, Taiwan (China); Chung, Chi-Jung [Department of Health Risk Management, College of Public Health, China Medical University and Hospital, Taichung, Taiwan (China); Department of Medical Research, China Medical University Hospital, Taichung, Taiwan (China); Huang, Chao-Yuan; Pu, Yeong-Shiau [Department of Urology, National Taiwan University Hospital, College of Medicine National Taiwan University, Taipei, Taiwan (China); Shiue, Horng-Sheng [Department of Chinese Medicine, Chang Gung Memorial Hospital, Taipei, Taiwan (China); Lai, Li-An [School of Public Health, College of Public Health and Nutrition, Taipei Medical University, Taipei, Taiwan (China); Lin, Ying-Chin [Department of Family Medicine, Shung Ho Hospital, Taipei Medical University, Taipei, Taiwan (China); Department of Health Examination, Wan Fang Hospital, Taipei Medical University, Taipei, Taiwan (China); Su, Chien-Tien [Department of Family Medicine, Taipei Medical University Hospital, Taipei, Taiwan (China); Hsueh, Yu-Mei, E-mail: ymhsueh@tmu.edu.tw [School of Public Health, College of Public Health and Nutrition, Taipei Medical University, Taipei, Taiwan (China); Department of Public Health, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan (China)

    2013-10-01

    Chronic exposure to arsenic can generate reactive oxidative species, which can induce certain proinflammatory cytokines such as tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6) and interleukin-8 (IL-8). TNF-α, IL-6 and IL-8 have been shown to be involved in the development and progression of various cancers, including bladder cancer. This study aimed to investigate the joint effect of the polymorphism of TNF-α − 308 G/A, IL-6 − 174 G/C, IL-8 − 251 T/A and urinary arsenic profiles on urothelial carcinoma (UC) risk. This study evaluated 300 pathologically-confirmed cases of UC and 594 cancer-free controls. Urinary arsenic species were detected using high-performance liquid chromatography-linked hydride generator and atomic absorption spectrometry. The polymorphism of TNF-α − 308 G/A, IL-6 − 174 G/C and IL-8 − 251 T/A was determined using polymerase chain reaction-restriction fragment length polymorphism. The joint effects on UC risk were estimated by odds ratios and 95% confidence intervals using unconditional logistic regression. We found that the TNF-α − 308 A/A and IL-8 − 251 T/T polymorphisms were significantly associated with UC. Moreover, significant dose–response joint effect of TNF-α − 308 A/A or IL-8 − 251 T/T genotypes and arsenic methylation indices were seen to affect UC risk. The present results also showed a significant increase in UC risk in subjects with the IL-8 − 251 T/T genotype for each SD increase in urinary total arsenic and MMA%. In contrast, a significant decrease in UC risk was found in subjects who carried the IL-8 − 251 T/T genotype for each SD increase in DMA%. - Highlights: • Joint effect of the TNF-α -308 A/A genotype and urinary total arsenic affected UC. • Joint effect of the IL-8 -251 T/T genotype and urinary total arsenic affected UC. • Urinary total arsenic level, TNF-α -308 A/A and IL-8 -251 T/T genotype affected UC.

  7. HCMV Infection Depress NGF Expression in Human Glioma Cells

    Institute of Scientific and Technical Information of China (English)

    Hai-tao WANG; Bin WANG; Zhi-jun LIU; Zhi-qiang BAI; Ling LI; Dong-meng QIAN; Zhi-yong YAN; Xu-xia SONG

    2009-01-01

    Human cytomegalovirus (HCMV) is the most common cause of congenital infection, resulting in birth defects such as microcephaly. In this study, RT-PCR and Western Blotting were performed to quantify the regulation of endogenic nerve growth factor expression in neuroglia cells by HCMV infection. The results showed that basal, endogenous NGF expression in U251 was unchanged during early HCMV infection. NGF expression is strongly down-regulated during the latent phase of infection. These results suggest that HCMV can depress the NGF expression in U251 cells.

  8. Molecular identification of bloodmeals from biting midges (Diptera: Ceratopogonidae; Culicoides Latreille) in Denmark

    DEFF Research Database (Denmark)

    Lassen, Sandra Boline; Nielsen, Søren A; Skovgård, Henrik;

    2011-01-01

    identified 251 blood engorged biting midges, and hosts were identified in 115 of 125 analysed specimens (90%). Cow, roe deer, horse, mallard and wood pigeon were identified as hosts. The most abundant host species was cow, which constituted 73.9% of the total identified bloodmeals, but the common wood pigeon...

  9. Effect of Platinum in Bifunctional Isomerization of n-Butane over Acid Zeolites

    Czech Academy of Sciences Publication Activity Database

    Babůrek, Evžen; Nováková, Jana

    2000-01-01

    Roč. 190, č. 1 (2000), s. 241-251. ISSN 0926-860X R&D Projects: GA AV ČR IAA4040710 Institutional research plan: CEZ:AV0Z4040901; CEZ:A54/98:Z4-040-9-ii Subject RIV: CF - Physical ; Theoretical Chemistry Impact factor: 1.576, year: 2000

  10. Pion freeze-out time in Pb plus Pb collisions at 158 AGeV/c studied via pi(-)/pi(+) and K-/K+ ratios

    Czech Academy of Sciences Publication Activity Database

    Aggarwal, M. M.; Petráček, Vojtěch; Šumbera, Michal

    2006-01-01

    Roč. 48, č. 2 (2006), s. 343-352. ISSN 1434-6044 R&D Projects: GA MŠk 1P04LA211 Institutional research plan: CEZ:AV0Z10480505 Keywords : heavy ion collisions * pion freeze -out * WA98 Subject RIV: BG - Nuclear, Atomic and Molecular Physics, Colliders Impact factor: 3.251, year: 2006

  11. Rose Atoll Site 27P 2/21/2012 2-3M

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — Onemetersquare 1 meter x 1 meter benthic substrate at Rose Atoll, site 27P 14 33.038S, 168 09.251W, between 2 and 3 meters along a permanent transect.

  12. Steinernema cholashanense n. sp. (Rhabditida, Steinernematidae) a new species of entomopathogenic nematode from the province of Sichuan, Chola Shan Mountains, China

    Czech Academy of Sciences Publication Activity Database

    Nguyen, K. B.; Půža, Vladimír; Mráček, Zdeněk

    2008-01-01

    Roč. 97, č. 3 (2008), s. 251-264. ISSN 0022-2011 Grant ostatní: European Commission(BE) COST 850 Institutional research plan: CEZ:AV0Z50070508 Source of funding: R - rámcový projekt EK Keywords : description * entomopathogenic nematode * insect parasite Subject RIV: EG - Zoology Impact factor: 2.005, year: 2008

  13. 77 FR 1670 - Gulf of Mexico Fishery Management Council (Council); Public Meetings

    Science.gov (United States)

    2012-01-11

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF COMMERCE... Atmospheric Administration (NOAA), Commerce. ACTION: Notice of public meetings. SUMMARY: The Gulf of Mexico... Street, Mobile, AL 36602; telephone: (251) 438-4000. Council address: Gulf of Mexico Fishery...

  14. Whole-genome sequencing of Salmonella enterica subsp. enterica serovar Cubana strains isolated from agricultural sources

    Science.gov (United States)

    We report draft genomes of Salmonella enterica subsp. enterica Serovar Cubana strain CVM42234 isolated from chick feed in 2012 and Salmonella Cubana strain 76814 isolated from swine in 2004. The genome sizes are 4,975,046 and 4,936,251 base pairs, respectively....

  15. The influence of tungsten compounds on the synthesis and properties of polyaniline

    Czech Academy of Sciences Publication Activity Database

    Stejskal, Jaroslav; Trchová, Miroslava; Holler, Petr; Sapurina, I.; Prokeš, J.

    2005-01-01

    Roč. 54, č. 12 (2005), s. 1606-1612. ISSN 0959-8103 R&D Projects: GA AV ČR IAA4050313; GA AV ČR IAA400500504 Institutional research plan: CEZ:AV0Z40500505 Keywords : aniline * conductivity * conducting polymer Subject RIV: BM - Solid Matter Physics ; Magnetism Impact factor: 1.251, year: 2005

  16. Varying levels of female promiscuity in four Apodemus mice species

    Czech Academy of Sciences Publication Activity Database

    Bryja, Josef; Patzenhauerová, Hana; Albrecht, Tomáš; Mošanský, L.; Stanko, M.; Stopka, P.

    2008-01-01

    Roč. 63, č. 2 (2008), s. 251-260. ISSN 0340-5443 R&D Projects: GA MŠk MEB090802; GA MŠk LC06073 Institutional research plan: CEZ:AV0Z60930519 Keywords : mating systems * multiple paternity * wood mice * testis size Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 2.917, year: 2008

  17. 7 CFR 1717.852 - Financing purposes.

    Science.gov (United States)

    2010-01-01

    ..., rehabilitation and integration of existing distribution facilities, equipment and systems, and associated service... Agriculture Regulations of the Department of Agriculture (Continued) RURAL UTILITIES SERVICE, DEPARTMENT OF..., equipment, and facilities include those listed in 7 CFR 1710.251(c) and 1710.252(c), as well as others...

  18. 41 CFR 101-25.103-3 - Trading stamps or bonus goods received from contractors.

    Science.gov (United States)

    2010-07-01

    ... 41 Public Contracts and Property Management 2 2010-07-01 2010-07-01 true Trading stamps or bonus... PROCUREMENT 25-GENERAL 25.1-General Policies § 101-25.103-3 Trading stamps or bonus goods received from contractors. When contracts contain a price reduction clause, any method (such as trading stamps or...

  19. Development of a radiochemical neutron activation analysis procedure for determination of rhenium in biological and environmental samples at ultratrace level

    Czech Academy of Sciences Publication Activity Database

    Kučera, Jan; Byrne, A. R.; Mizera, Jiří; Lučaníková, M.; Řanda, Zdeněk

    2006-01-01

    Roč. 269, č. 2 (2006), s. 251-257. ISSN 0236-5731 R&D Projects: GA ČR(CZ) GA203/04/0943 Institutional research plan: CEZ:AV0Z10480505 Keywords : radiochemical neutron activation analysis * rhenium * biological and environmental samples Subject RIV: CB - Analytical Chemistry, Separation Impact factor: 0.509, year: 2006

  20. Effect of main belt perturbers on asteroid-pair age estimation

    Czech Academy of Sciences Publication Activity Database

    Galád, Adrián

    2012-01-01

    Roč. 548, November (2012), A25/1-A25/7. ISSN 0004-6361 R&D Projects: GA ČR GAP209/12/0229 Institutional support: RVO:67985815 Keywords : minor planets * asteroids * celestial mechanics Subject RIV: BN - Astronomy, Celestial Mechanics, Astrophysics Impact factor: 5.084, year: 2012

  1. Thermal stresses in small meteoroids II. Effects of an insulating surface layer

    Czech Academy of Sciences Publication Activity Database

    Čapek, David; Vokrouhlický, D.

    2012-01-01

    Roč. 539, March (2012), A25/1-A25/10. ISSN 0004-6361 R&D Projects: GA ČR GP205/09/P455 Institutional research plan: CEZ:AV0Z10030501 Keywords : minor planets * asteroids Subject RIV: BN - Astronomy, Celestial Mechanics, Astrophysics Impact factor: 5.084, year: 2012

  2. Reduction of surface subsidence risk by fly ash exploitation as filling material in deep mining areas

    Czech Academy of Sciences Publication Activity Database

    Trčková, Jiřina; Šperl, Jan

    2010-01-01

    Roč. 53, č. 2 (2010), s. 251-258. ISSN 0921-030X Institutional research plan: CEZ:AV0Z30460519 Keywords : undermining * subsidence of the surface * impact reduction Subject RIV: DO - Wilderness Conservation Impact factor: 1.398, year: 2010 www.springerlink.com/content/y8257893528lp56w/

  3. A new house bat species (Scotophilus, Vespertilionidae) from East Africa: resurrection of\

    Czech Academy of Sciences Publication Activity Database

    Vallo, Peter; Reeder, D. M.; Benda, P.

    Brno: Ústav biologie obratlovců AV ČR, 2015 - (Bryja, J.; Řehák, Z.; Zukal, J.). s. 251 ISBN 978-80-87189-18-4. [Zoologické dny. 12.02.2015-13.02.2015, Brno] Institutional support: RVO:68081766 Keywords : house bat species * East Africa Subject RIV: EG - Zoology

  4. 77 FR 61401 - Notice of Availability of Government-Owned Inventions; Available for Licensing

    Science.gov (United States)

    2012-10-09

    ... BOW SKIRT RETRACTION SYSTEM//Patent No. 7,712,429: LAUNCH AND RECOVERY SYSTEM FOR UNMANNED UNDERSEA...// Patent No. 7,735,781: METHOD AND SYSTEM FOR DEPLOYMENT OF ORDNANCE FROM AN AIRCRAFT IN MID-FLIGHT//Patent...,251: MAGNETIC ANOMALY SENSING SYSTEM FOR DETECTION, LOCALIZATION AND CLASSIFICATION OF A...

  5. Constructive lower bounds for off-diagonal Ramsey numbers

    Czech Academy of Sciences Publication Activity Database

    Alon, N.; Pudlák, Pavel

    2001-01-01

    Roč. 122, č. 3 (2001), s. 243-251. ISSN 0021-2172 R&D Projects: GA AV ČR IAA1019901; GA MŠk ME 103 Institutional research plan: CEZ:AV0Z1019905; CEZ:AV0Z1019905 Keywords : Ramsey's theorem * graph Subject RIV: BA - General Mathematics Impact factor: 0.545, year: 2001

  6. Time-dependent mechanical characteristics measured through the use of a microindentation technique

    Czech Academy of Sciences Publication Activity Database

    Minster, Jiří; Bláhová, O.; Lukeš, J.; Němeček, J.

    2010-01-01

    Roč. 14, č. 3 (2010), s. 243-251. ISSN 1385-2000 R&D Projects: GA AV ČR(CZ) IAA200710801 Institutional research plan: CEZ:AV0Z20710524 Keywords : material s testing * microindentation * viscoelastic compliance * relaxation modulus Subject RIV: JI - Composite Material s Impact factor: 0.955, year: 2010

  7. 48 CFR 951.7002 - Responsibilities.

    Science.gov (United States)

    2010-10-01

    ... GOVERNMENT SOURCES BY CONTRACTORS Contractor Employee Travel Discounts 951.7002 Responsibilities. The contracting officer shall insert the clause at 952.251-70, Contractor employee travel discounts, in all cost-reimbursable solicitations and contracts when significant costs for rail travel, car rental, or lodging will...

  8. 76 FR 10755 - Establishment of Office of the Chief Scientist; Revision of Delegations of Authority

    Science.gov (United States)

    2011-02-28

    ..., Director, Office of the Chief Scientist, United States Department of Agriculture, 1400 Independence Ave... Department) and the Director of the Office of the Chief Scientist with respect to scientific integrity within.... OCS is headed by a Director that reports to the Under Secretary for REE. Pursuant to section 251(c)...

  9. Identification similar species of Dickeya by gel format of the electromigration techniques

    Czech Academy of Sciences Publication Activity Database

    Kubesová, Anna; Šalplachta, Jiří; Horký, J.; Horká, Marie

    Messina : Chromaleont S.r.L, 2012 - (Sandra, P.; Mondello, L.). s. 251 [International Symposium on Capillary Chromatography /36./ and GC x GC Symposium /9./. 27.5.2012-01.06.2012, Riva del Garda] Institutional research plan: CEZ:AV0Z40310501 Keywords : Dickeya * isoelectric focusing * gel electrophoresis * fingerprint Subject RIV: CB - Analytical Chemistry, Separation

  10. Modelling of Continual Induction Hardening in Quasi-Coupled Formulation

    Czech Academy of Sciences Publication Activity Database

    Barglik, J.; Doležel, Ivo; Karban, P.; Ulrych, B.

    2005-01-01

    Roč. 24, č. 1 (2005), s. 251-260. ISSN 0332-1649 Grant ostatní: PSRC(PL) 4T08C 04823 Institutional research plan: CEZ:AV0Z20570509 Keywords : mathematical modelling * electromagnetism * induction Subject RIV: JA - Electronics ; Optoelectronics, Electrical Engineering Impact factor: 0.188, year: 2005

  11. Attenuation of morphine antinociceptive tolerance by cannabinoid CB1 and CB2 receptor antagonists.

    Science.gov (United States)

    Altun, Ahmet; Yildirim, Kemal; Ozdemir, Ercan; Bagcivan, Ihsan; Gursoy, Sinan; Durmus, Nedim

    2015-09-01

    Cannabinoid CB1 and CB2 receptor antagonists may be useful for their potential to increase or prolong opioid analgesia while attenuating the development of opioid tolerance. The aim of this study was to investigate the effects of AM251 (a selective CB1 antagonist) and JTE907 (a selective CB2 antagonist) on morphine analgesia and tolerance in rats. Adult male Wistar albino rats weighing 205-225 g were used in these experiments. To constitute morphine tolerance, we used a 3 day cumulative dosing regimen. After the last dose of morphine was injected on day 4, morphine tolerance was evaluated by analgesia tests. The analgesic effects of morphine (5 mg/kg), ACEA (a CB1 receptor agonist, 5 mg/kg), JWH-015 (a CB2 receptor agonist, 5 mg/kg), AM251 (1 mg/kg) and JTE907 (5 mg/kg) were considered at 30-min intervals (0, 30, 60, 90, and 120 min) by tail-flick and hot-plate analgesia tests. Our findings indicate that ACEA and JWH907 significantly increased morphine analgesia and morphine antinociceptive tolerance in the analgesia tests. In contrast, the data suggested that AM251 and JTE907 significantly attenuated the expression of morphine tolerance. In conclusion, we observed that co-injection of AM251 and JTE907 with morphine attenuated expression of tolerance to morphine analgesic effects and decreased the morphine analgesia. PMID:25894754

  12. Repeatless and Repeat-Based Centromeres in Potato: Implications for Centromere Evolution[C][W

    Czech Academy of Sciences Publication Activity Database

    Gong, Z.; Wu, Y.; Koblížková, Andrea; Torres, G.A.; Wang, K.; Iovene, M.; Neumann, Pavel; Zhang, W.; Novák, Petr; Buell, C.R.; Macas, Jiří; Jiang, J.

    2012-01-01

    Roč. 24, č. 9 (2012), s. 3559-3574. ISSN 1040-4651 R&D Projects: GA MŠk(CZ) LH11058 Institutional research plan: CEZ:AV0Z50510513 Institutional support: RVO:60077344 Keywords : repetitive sequences * plant satellite repeats * Arabidopsis thaliana * rice centromere * wild potatoes Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 9.251, year: 2012

  13. 76 FR 54467 - Economic Impact Policy

    Science.gov (United States)

    2011-09-01

    ... From the Federal Register Online via the Government Publishing Office EXPORT-IMPORT BANK OF THE UNITED STATES Economic Impact Policy This notice is to inform the public that the Export-Import Bank of the United States has received an application for a $25.1 million guarantee to support the U.S....

  14. 47 CFR 101.105 - Interference protection criteria.

    Science.gov (United States)

    2010-10-01

    ... parties are provided a C/I protection ratio of 0 dB. An applicant proposing to operate at an AAT greater... EIRP exceed 70 dBm on the 10.6 GHz channels: AAT (meters) EIRP dBm Above 300 +38 251 to 300 41 201...

  15. Acidification in European mountain lake districts: A regional assessment of critical load exceedance

    Czech Academy of Sciences Publication Activity Database

    Curtis, CH. J.; Botev, I.; Camarero, L.; Catalan, J.; Cogalniceanu, D.; Hughes, M.; Kernan, M.; Kopáček, Jiří; Korhola, A.; Psenner, R.; Rogora, M.; Stuchlík, E.; Veronesi, M.; Wright, R. F.

    2005-01-01

    Roč. 67, č. 3 (2005), s. 237-251. ISSN 1015-1621 Grant ostatní: EC(XE) EMERGE EVK1-CT-1999-00032 Institutional research plan: CEZ:AV0Z60170517 Keywords : FAB model * sulphur * nitrogen Subject RIV: DJ - Water Pollution ; Quality Impact factor: 1.662, year: 2005

  16. Allergic contact dermatitis in Danish children referred for patch testing - a nationwide multicentre study

    DEFF Research Database (Denmark)

    Simonsen, Anne Birgitte; Deleuran, Mette; Mortz, Charlotte Gotthard;

    2013-01-01

    -17 years, 25.1% had one or more positive patch test reactions. The associated relevance was 66.4%. The most common sensitizers were metals, fragrances, and hair dyes. The frequency of positive patch test reactions and allergic contact dermatitis was significantly higher among girls. CONCLUSIONS: Allergic...

  17. 78 FR 15007 - Notice of Open House-Draft Environmental Impact Statement for Revised Water Control Manuals for...

    Science.gov (United States)

    2013-03-08

    ... Revised Water Control Manuals for the Alabama-Coosa-Tallapoosa River Basin AGENCY: Department of the Army... Alabama-Coosa-Tallapoosa River Basin (ACT) Water Control Master Manual (Master Manual). The public comment... CONTACT: Mr. Lewis Sumner at telephone (251) 694-3857. SUPPLEMENTARY INFORMATION: The Master...

  18. Sequence Classification: 783456 [

    Lifescience Database Archive (English)

    Full Text Available Non-TMB Non-TMH Non-TMB Non-TMB Non-TMB Non-TMB >gi|17560486|ref|NP_505710.1| abnormal CATecholamine distrib...ution CAT-4, GTP cyclohydrolase I (25.1 kD) (cat-4) || http://www.ncbi.nlm.nih.gov/protein/17560486 ...

  19. Influence of Non-Perfect Step Input Concentration at the Feed Side of the Membrane Surface on the Diffusion Coefficient Evaluation

    Czech Academy of Sciences Publication Activity Database

    Čermáková, Jiřina; Fialová, Kateřina; Petričkovič, Roman; Kudrna, V.; Uchytil, Petr

    2006-01-01

    Roč. 15, č. 3 (2006), s. 246-251. ISSN 1022-1344 R&D Projects: GA AV ČR(CZ) 1QS401250509; GA AV ČR(CZ) IAA4072402 Institutional research plan: CEZ:AV0Z40720504 Keywords : diffusion coefficient * flux * dispersion Subject RIV: CI - Industrial Chemistry, Chemical Engineering Impact factor: 1.073, year: 2006

  20. Effects of melatonin on ischemia and reperfusion injury of the rat heart

    Czech Academy of Sciences Publication Activity Database

    Szárszoi, Ondrej; Asemu, Girma; Vaněček, Jiří; Ošťádal, Bohuslav; Kolář, František

    2001-01-01

    Roč. 15, č. 3 (2001), s. 251-257. ISSN 0920-3206 R&D Projects: GA ČR GA306/98/0470; GA MŠk LN00A069 Institutional research plan: CEZ:AV0Z5011922 Keywords : melatonin * heart * ischemia Subject RIV: ED - Physiology Impact factor: 1.009, year: 2001

  1. Simplicial Finite Elements in Higher Dimensions

    Czech Academy of Sciences Publication Activity Database

    Brandts, J.; Korotov, S.; Křížek, Michal

    2007-01-01

    Roč. 52, č. 3 (2007), s. 251-265. ISSN 0862-7940 R&D Projects: GA ČR GA201/04/1503 Institutional research plan: CEZ:AV0Z10190503 Keywords : n- simplex * finite element method * superconvergence Subject RIV: BA - General Mathematics

  2. Počítače a internet v historickém výzkumu raného novověku

    Czech Academy of Sciences Publication Activity Database

    Mikulec, Jiří

    Praha: Nakladatelství Lidové Noviny, FF UK, 2013, s. 102-116. (České dějiny. 6). ISBN 978-80-7422-251-1 Institutional support: RVO:67985963 Keywords : Computers * Internet * Historical Research Subject RIV: AB - History

  3. 7 CFR 250.67 - Charitable institutions.

    Science.gov (United States)

    2010-01-01

    ... established in accordance with 7 CFR 251.4(h)(4); or (3) Other similar data. (d) Types and quantities of... 7 Agriculture 4 2010-01-01 2010-01-01 false Charitable institutions. 250.67 Section 250.67... Charitable institutions. (a) Distribution to charitable institutions. The Department provides donated...

  4. Identification of tuberculosis bacteria based on shape and color

    Czech Academy of Sciences Publication Activity Database

    Forero, M. G.; Šroubek, Filip; Cristóbal, G.

    2004-01-01

    Roč. 10, č. 4 (2004), s. 251-262. ISSN 1077-2014 R&D Projects: GA ČR GA102/04/0155 Institutional research plan: CEZ:AV0Z1075907 Keywords : tuberculosis * fluorescence microscopy * clustering Subject RIV: BD - Theory of Information Impact factor: 0.455, year: 2004

  5. Archduke Ferdinand II of Tyrol, Royal Governor in the Bohemian Lands

    Czech Academy of Sciences Publication Activity Database

    Fučíková, E.; Muchka, Ivan; Halata, M.

    Prague : Prague Castle Administration, 2003, s. 251-257. ISBN 80-86161-73-0 R&D Projects: GA AV ČR KSK9056118 Institutional research plan: CEZ:AV0Z8033913 Keywords : Prague Castle * Renaissance architecture * lawcourt Subject RIV: AL - Art, Architecture, Cultural Heritage

  6. Zástupcem svého otce v Českých zemích: arcivévoda Ferdinand II. zvaný Tyrolský

    Czech Academy of Sciences Publication Activity Database

    Fučíková, E.; Muchka, Ivan; Halata, M.

    Praha : Správa Pražského hradu, 2003, s. 251-257. ISBN 80-86161-72-2 R&D Projects: GA AV ČR KSK9056118 Institutional research plan: CEZ:AV0Z8033913 Keywords : Prague Castle * Renaissance architecture * lawcourt Subject RIV: AL - Art, Architecture, Cultural Heritage

  7. The Impact of Imports and Exports on a Country's Quality of Life

    Science.gov (United States)

    Sirgy, M. Joseph; Lee, Dong-Jin; Miller, Chad; Littlefield, James E.; Atay, Eda Gurel

    2007-01-01

    This paper is a sequel to Sirgy et al. ("Social Ind. Res." 68(3) (2004) 251), "The Impact of Globalization on a Country's Quality of Life: Toward an Integrated Model" published in "Social Indicators Research." That paper conceptualized globalization in terms of the free flow of four major components: (1) goods and services, (2) people, (3)…

  8. Modeling of Throttling Process inside Capillary Tube

    Czech Academy of Sciences Publication Activity Database

    Vinš, Václav; Vacek, V.

    Praha : ČVUT, 2009, s. 250-251. ISBN 978-80-01-04286-1. [ANNUAL CTU UNIVERSITY-WIDE SEMINAR /18./. Praha (CZ), 16.02.2009-20.02.2009] Institutional research plan: CEZ:AV0Z20760514 Keywords : capillary tube * numerical model * metastable flow Subject RIV: BJ - Thermodynamics https://workshop.cvut.cz/2009/

  9. 48 CFR 7.102 - Policy.

    Science.gov (United States)

    2010-10-01

    ... conduct market research (see part 10) for all acquisitions in order to promote and provide for— (1....S.C. 251, et seq.); and (2) Full and open competition (see part 6) or, when full and open competition is not required in accordance with part 6, to obtain competition to the maximum extent...

  10. AcEST: DK959149 [AcEST

    Lifescience Database Archive (English)

    Full Text Available TST39A01NGRL0003_O12 651 Adiantum capillus-veneris mRNA similar to SNF1-related protein kinase D ... -KNMEM 83 Query: 266 EEKVRREIKILRLFMHTHIIRLYEVIETETDIY FVMEYVNSGELFDHIVEKGRLSEDEAR 445 EEKVRREIKILRLFMH HI ... IRLYEVIET TDIY ... VMEYVNSGELFD+IVEKGRL EDEAR Sbjct: 84 EEKVRREIKILRL ... FMHPHIIRLYEVIETPTDIY LVMEYVNSGELFDYIVEKGRLQEDEAR 143 Query: 446 RFFQQIIS ... - 56 Query: 251 RNADMEEKVRREIKILRLFMHTHIIRLYEVIETETDIY FVMEYVNSGELFDHIVEKGRLS 430 +N +MEEKVRREIKILRLFMH HI ...

  11. AcEST: DK962867 [AcEST

    Lifescience Database Archive (English)

    Full Text Available TST39A01NGRL0015_A05 639 Adiantum capillus-veneris mRNA similar to SNF1-related protein kinase D ... -KNMEM 83 Query: 266 EEKVRREIKILRLFMHTHIIRLYEVIETETDIY FVMEYVNSGELFDHIVEKGRLSEDEAR 445 EEKVRREIKILRLFMH HI ... IRLYEVIET TDIY ... VMEYVNSGELFD+IVEKGRL EDEAR Sbjct: 84 EEKVRREIKILRL ... FMHPHIIRLYEVIETPTDIY LVMEYVNSGELFDYIVEKGRLQEDEAR 143 Query: 446 RFFQQIIS ... - 56 Query: 251 RNADMEEKVRREIKILRLFMHTHIIRLYEVIETETDIY FVMEYVNSGELFDHIVEKGRLS 430 +N +MEEKVRREIKILRLFMH HI ...

  12. Tectonic phase separation applied to the Sudetic Marginal Fault Zone (NE part of the Czech Republic)

    Czech Academy of Sciences Publication Activity Database

    Nováková, Lucie

    2015-01-01

    Roč. 12, č. 2 (2015), s. 251-267. ISSN 1672-6316 R&D Projects: GA ČR GA205/09/1244 Institutional support: RVO:67985891 Keywords : Sudetic Marginal Fault Zone * paleostress reconstruction * active tectonics * frequency analysis Subject RIV: DC - Siesmology, Volcanology, Earth Structure Impact factor: 0.963, year: 2014

  13. Mechanism of heavy ion radiation-induced cancer cell death

    International Nuclear Information System (INIS)

    We previously reported that the carbon beam triggers apoptosis in radio-resistant cancer cell lines via extracellular signal-regulated kinase (ERK)- and mitochondrial Bcl-2 family protein-dependant mechanism. Here, we further examined the further apoptosis-inducing mechanism of carbon beam in two glioma cell lines (T98G, U251). ERK1/2 knockdown experiments revealed that ERK regulates this apoptosis-inducing machinery upstream of mitochondria. Furthermore, we also found that both T98G cell and U251 cell stably expressing dominant-negative ERK2 suppress cell death induced by carbon beam irradiation. We also found proapoptotic PUMA and antiapoptotic Bcl-2 dynamically chang their expression levels corresponding to ERK activation after CB irradiation in U251 cell, and knockdown of PUMA decreased CB-induced U251 cell death. These data suggest that kinase action of ERK is essential for CB-induced glioma cell death, and proapoptotic PUMA and antiapoptotic Bcl-2 might be downstream targets of ERK in CB-induced glioma cell death mechanism. (author)

  14. Congenital heart disease

    Institute of Scientific and Technical Information of China (English)

    1997-01-01

    970296 Evaluating the degree of pulmonary vascularlesions in congenital heart disease with selective pul-monary angiography. PAN Shiwei(潘世伟), et al.Fuwai Hosp, CAMS & PUMC, Beijing, 100037. Chin JCardiol 1997; 25(1): 39-41. Objective: To evaluate the degree of pulmonary vas-

  15. Preparation and characterization of ZnS nanoparticles deposited on montmorillonite

    Czech Academy of Sciences Publication Activity Database

    Kozák, O.; Praus, P.; Kočí, K.; Klementová, Mariana

    2010-01-01

    Roč. 352, č. 2 (2010), s. 244-251. ISSN 0021-9797 Institutional research plan: CEZ:AV0Z40320502 Keywords : ZnS nanoparticles * montmorillonite * photoluminescence * photocatalysis * carbon dioxide Subject RIV: CA - Inorganic Chemistry Impact factor: 3.068, year: 2010

  16. Gas barrier properties of nanocomposites based on in situ polymerized poly(n-butyl methacrylate) in the presence of surface modified montmorillonite

    Czech Academy of Sciences Publication Activity Database

    Herrera-Alonso, J. M.; Sedláková, Zdeňka; Marand, E.

    2010-01-01

    Roč. 349, 1-2 (2010), s. 251-257. ISSN 0376-7388 R&D Projects: GA AV ČR KAN100500651; GA MŠk ME09058 Institutional research plan: CEZ:AV0Z40500505 Keywords : barrier membranes * nanocomposites * montmorillonite Subject RIV: CD - Macromolecular Chemistry Impact factor: 3.673, year: 2010

  17. Mechanisms Linking Violence Exposure to Health Risk Behavior in Adolescence: Motivation to Cope and Sensation Seeking

    Science.gov (United States)

    Brady, Sonya S.; Donenberg, Geri R.

    2006-01-01

    Objective: This study examined two potential mechanisms linking violence exposure and health risk behavior among adolescents in psychiatric care: sensation seeking and coping with stress through escape behavior. Method: Male (59%) and female adolescents (N = 251), ages 12 to 19 years, from diverse ethnic backgrounds (61% African American, 19%…

  18. Trans-ancestry genome-wide association study identifies 12 genetic loci influencing blood pressure and implicates a role for DNA methylation

    DEFF Research Database (Denmark)

    Kato, Norihiro; Loh, Marie; Takeuchi, Fumihiko;

    2015-01-01

    We carried out a trans-ancestry genome-wide association and replication study of blood pressure phenotypes among up to 320,251 individuals of East Asian, European and South Asian ancestry. We find genetic variants at 12 new loci to be associated with blood pressure (P = 3.9 × 10(-11) to 5.0 × 10(...

  19. Trajectories of Children's Internalizing Symptoms: The Role of Maternal Internalizing Symptoms, Respiratory Sinus Arrhythmia and Child Sex

    Science.gov (United States)

    Wetter, Emily K.; El-Sheikh, Mona

    2012-01-01

    Background: We assessed trajectories of children's internalizing symptoms as predicted by interactions among maternal internalizing symptoms, respiratory sinus arrhythmia (RSA) and child sex. Method: An ethnically and socioeconomically diverse sample of children (n = 251) participated during three study waves. Children's mean ages were 8.23 years…

  20. Mortaliteten hos multitraumatiserede trafiktilskadekomne. Deskriptiv undersøgelse 1986-1990

    DEFF Research Database (Denmark)

    Larsen, M S; Jørgensen, H R

    1995-01-01

    In the period 1986-1990, 16,307 patients were injured in road traffic accidents in the city of Odense, Denmark. 251 had multiple injuries according to the definition: AIS > or = 3 in at least two body regions. Overall mortality was 41%, while the mortality in hospital was 20%. 19 patients died...

  1. Structural changes of UHMWPE after e-beam irradiation and thermal treatment

    Czech Academy of Sciences Publication Activity Database

    Šlouf, Miroslav; Synková, Hana; Baldrian, Josef; Marek, Antonín; Kovářová, Jana; Schmidt, Pavel; Dorschner, H.; Stephan, M.; Gohs, U.

    85B, č. 1 (2008), s. 240-251. ISSN 1552-4973 R&D Projects: GA ČR GA106/04/1118; GA MŠk 2B06096 Institutional research plan: CEZ:AV0Z40500505 Keywords : UHMWPE supramolecular structure * e-beam irradiation * thermal treatment Subject RIV: CD - Macromolecular Chemistry Impact factor: 2.030, year: 2008

  2. Ixodes ricinus and its transmitted pathogens in urban and peri-urban areas in Europe: new hazards and relevance for public health

    Czech Academy of Sciences Publication Activity Database

    Rizzoli, A.; Silaghi, C.; Obiegala, A.; Rudolf, Ivo; Hubálek, Zdeněk; Földvári, G.; Plantard, O.; Vayssier-Taussat, M.; Bonnet, S.; Špitalská, E.; Kazimírová, M.

    2014-01-01

    Roč. 2, č. 251 (2014). ISSN 2296-2565 EU Projects: European Commission(XE) 261504 - EDENEXT Institutional support: RVO:68081766 Keywords : ticks * Ixodes ricinus * tick-borne pathogens * urban habitats * Europe Subject RIV: FN - Epidemiology, Contagious Diseases ; Clinical Immunology

  3. Renal Na,K-ATPase during experimental NO-deficient hypertension in young and adults rats

    Czech Academy of Sciences Publication Activity Database

    Javorková, V.; Vlkovičová, J.; Kuneš, Jaroslav; Pecháňová, Olga; Zicha, Josef; Vrbjar, N.

    Bratislava : Advent- Orion , 2007 - (Pecháňová, O.), s. 246-251 ISBN 978-80-8071-094-1 R&D Projects: GA MŠk(CZ) 1M0510 Institutional research plan: CEZ:AV0Z50110509 Keywords : maturation of rats * Na,K-ATPase * NO-deficient hypertension Subject RIV: FA - Cardiovascular Diseases incl. Cardiotharic Surgery

  4. Double Dissociation of Monoacylglycerol Lipase Inhibition and CB1 Antagonism in the Central Amygdala, Basolateral Amygdala, and the Interoceptive Insular Cortex on the Affective Properties of Acute Naloxone-Precipitated Morphine Withdrawal in Rats.

    Science.gov (United States)

    Wills, Kiri L; Petrie, Gavin N; Millett, Geneva; Limebeer, Cheryl L; Rock, Erin M; Niphakis, Micah J; Cravatt, Benjamin F; Parker, Linda A

    2016-06-01

    Both CB1 receptor antagonism and agonism, in particular by 2-arachidonyl glycerol (2-AG), have been shown to reduce somatic symptoms of morphine withdrawal (MWD). Here we evaluated the effects of both systemic pretreatment with the monoacylglycerol lipase (MAGL) inhibitor MJN110 (which selectively elevates 2-AG) and central administration of both MJN110 and the CB1 antagonist (AM251) on the affective properties of MWD. Acute MWD induced place aversion occurs when naloxone is administered 24 h following a single exposure to a high dose of morphine. Systemic pretreatment with the MAGL inhibitor, MJN110, prevented the aversive effects of acute MWD by a CB1 receptor-dependent mechanism. Furthermore, in a double dissociation, AM251 infusions into the central amygdala, but MJN110 infusions into the basolateral amygdala, interfered with the naloxone-precipitated MWD induced place aversion. As well, MJN110, but not AM251, infusions into the interoceptive insular cortex (a region known to be activated in acute MWD) also prevented the establishment of the place aversion by a CB1 mechanism of action. These findings reveal the respective sites of action of systemically administered MJN110 and AM251 in regulating the aversive effects of MWD. PMID:26647976

  5. Determination of lycopene in food by on-line SFE-LC eliminating its degradation during the analytical procedure

    Czech Academy of Sciences Publication Activity Database

    Pól, Jaroslav; Hyötyläinen, T.; Ranta-Aho, O.; Riekkola, M. L.

    2005-01-01

    Roč. 99, S (2005), s251. ISSN 0009-2770. [Meeting on Chemistry and Life /3./. 20.09.2005-22.09.2005, Brno] R&D Projects: GA AV ČR KJB4031405 Keywords : liquid chromatography * supercritical fluid extraction * antioxidant Subject RIV: CB - Analytical Chemistry, Separation

  6. 77 FR 36251 - Intermountain Region, Boise National Forest; Emmett Ranger District, Idaho; Scriver Creek...

    Science.gov (United States)

    2012-06-18

    ... (FR Vol. 76, No. 251; NOA for EIS No. 20110438). Specifically, an SDEIS is needed to identify that a... (NOA) of the SDEIS in the Federal Register. The publication date in the Federal Register is the only means for calculating the comment ] period for the SDEIS. Based on an anticipated NOA publication...

  7. Molecular evidence for polyphyletic origin of the primary symbionts of sucking lice (Phthiraptera, Anoplura)

    Czech Academy of Sciences Publication Activity Database

    Hypša, Václav; Křížek, J.

    2007-01-01

    Roč. 54, č. 2 (2007), s. 242-251. ISSN 0095-3628 Grant ostatní: GA ČR(CZ) GA206/04/0520 Institutional research plan: CEZ:AV0Z60220518 Keywords : Anoplura * endosymbionts * coevolution * molecular phylogeny Subject RIV: EE - Microbiology, Virology Impact factor: 2.558, year: 2007

  8. Regulation of phosphoenolpyruvate carboxylase in PVYNTN-infected tobacco plants

    Czech Academy of Sciences Publication Activity Database

    Müller, Karel; Doubnerová, V.; Synková, Helena; Čeřovská, Noemi; Ryšlavá, H.

    2009-01-01

    Roč. 390, č. 3 (2009), s. 245-251. ISSN 1431-6730 R&D Projects: GA ČR GA206/03/0310; GA MŠk 1M0505 Institutional research plan: CEZ:AV0Z50380511 Keywords : biotic stress * Nicotiana tabacum * phosphoenolpyruvate carboxylase (PEPC) Subject RIV: ED - Physiology Impact factor: 2.732, year: 2009

  9. 24 CFR 248.201 - Definitions.

    Science.gov (United States)

    2010-04-01

    ... 24 CFR part 5. Adjusted Income. Annual income, as specified in § 251.21 of this chapter, less... Commissioner under part 221 of this chapter and bearing a below market interest rate as provided under § 221... allowable distributions. Return on Investment. The amount of allowable distributions, tax benefits,...

  10. Drug: D06126 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available 252.164 D06126.gif Antihypertensive CAS: 51274-83-0 PubChem: 47207784 LigandBox: D06126 NIKKAJI: J268.948K A...D06126 Drug Tiamenidine hydrochloride (USAN); Symcor (TN) C8H10ClN3S. HCl 251.0051

  11. File list: ALL.Bld.05.AllAg.Leukemic_bone_marrow [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available ALL.Bld.05.AllAg.Leukemic_bone_marrow mm9 All antigens Blood Leukemic bone marrow S...251 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/ALL.Bld.05.AllAg.Leukemic_bone_marrow.bed ...

  12. The Apis mellifera filamentous virus genome

    Science.gov (United States)

    A complete reference genome of the Apis mellifera Filamentous virus (AmFV) was determined using Illumina Hiseq sequencing. The AmFV genome is a double strand DNA molecule of approximately 498’500 nucleotides with a GC content of 50.8%. It encompasses 251 non overlapping open reading frames (ORFs), e...

  13. Students' Facebook Usage and Academic Achievement: A Case Study of Private University in Thailand

    Science.gov (United States)

    Sereetrakul, Wilailuk

    2013-01-01

    The objective of this study was to determine if the time spent on Facebook and the purpose for which Facebook was used had any impact on the academic achievement of the students. This exploratory research used a questionnaire to collect data from 251 undergraduate students at a private university in Bangkok, Thailand. Data were analyzed using…

  14. Facebook Use and the Tendency to Ruminate among College Students: Testing Mediational Hypotheses

    Science.gov (United States)

    Locatelli, Sara M.; Kluwe, Katharina; Bryant, Fred B.

    2012-01-01

    Studies have found that general use of Facebook influences subjective well-being. However, fewer studies have explored the impact of specific use behaviors, such as information posted in status updates. The current study uses data collected from 251 Facebook-using undergraduate students through an online survey, and examines the valence and…

  15. Cognitive Functioning in Affected Sibling Pairs with ADHD: Familial Clustering and Dopamine Genes

    Science.gov (United States)

    Loo, Sandra K.; Rich, Erika Carpenter; Ishii, Janeen; McGough, James; McCracken, James; Nelson, Stanley; Smalley, Susan L.

    2008-01-01

    Background: This paper examines familiality and candidate gene associations of cognitive measures as potential endophenotypes in attention-deficit/hyperactivity disorder (ADHD). Methods: The sample consists of 540 participants, aged 6 to 18, who were diagnosed with ADHD from 251 families recruited for a larger genetic study of ADHD. All members of…

  16. Sr-sulphate and associated minerals found from kyanite-bearing eclogite in the Moldanubian Zone of the Bohemian Massif, Czech Republic

    Czech Academy of Sciences Publication Activity Database

    Nakamura, D.; Kobayshi, T.; Shimobayashi, N.; Svojtka, Martin; Hirajima, T.

    2010-01-01

    Roč. 105, č. 5 (2010), s. 251-261. ISSN 1345-6296 Grant ostatní: Kyoto University(JP) 14400313 Institutional research plan: CEZ:AV0Z30130516 Keywords : celestine * Sr * eclogite * sulphide * Bohemian Massif Subject RIV: DB - Geology ; Mineralogy Impact factor: 0.677, year: 2010

  17. Kapalinová extrakce fytosterolů a dalších cenných látek z tálových mýdel

    Czech Academy of Sciences Publication Activity Database

    Rousková, Milena; Heyberger, Aleš; Tříska, Jan; Krtička, M.

    2011-01-01

    Roč. 105, č. 4 (2011), s. 251-255. ISSN 0009-2770 Institutional research plan: CEZ:AV0Z40720504; CEZ:AV0Z60870520 Keywords : liquid-liquid extraction * tall soap * phytosterols Subject RIV: CI - Industrial Chemistry, Chemical Engineering Impact factor: 0.529, year: 2011

  18. Strongly regular family of boundary-fitted tetrahedral meshes of bounded C^2 domains

    Czech Academy of Sciences Publication Activity Database

    Hošek, Radim

    2016-01-01

    Roč. 61, č. 3 (2016), s. 233-251. ISSN 0862-7940 EU Projects: European Commission(XE) 320078 - MATHEF Keywords : boundary fitted mesh * strongly regular family * Sommerville tetrahedron Subject RIV: BA - General Mathematics Impact factor: 0.400, year: 2014 http://hdl.handle.net/10338.dmlcz/145699

  19. Optically transparent diamond-PDMS microfluidic system for electronic monitoring of cells

    Czech Academy of Sciences Publication Activity Database

    Babchenko, Oleg; Kromka, Alexander; Conde, J.P.; Chu, V.; Schmiedinger, T.; Rezek, Bohuslav

    2014-01-01

    Roč. 251, č. 12 (2014), s. 2593-2598. ISSN 0370-1972 R&D Projects: GA ČR GAP108/12/0996 Institutional support: RVO:68378271 Keywords : cells culturing * diamond sensor * electrical characterization * microfluidic system * optical monitoring * surface conductivity Subject RIV: BO - Biophysics Impact factor: 1.489, year: 2014

  20. QTL Information Table: 366 [Q-TARO

    Lifescience Database Archive (English)

    Full Text Available qGL-6 Morphological trait Seed Grain length RFLP C)Interval BIL Nipponbare Kasalath B R674 R2549 ... H.Q. (2003). Mapping quantitative trait loci underlyi ng appearance quality of rice grains (Oryza sativa ... L.). Yi ... Chuan Xue Bao 30, 251-259. ...

  1. QTL Information Table: 367 [Q-TARO

    Lifescience Database Archive (English)

    Full Text Available qGW-3 Morphological trait Seed Grain width RFLP C)Interval BIL Nipponbare Kasalath B G332 C80 ph ... H.Q. (2003). Mapping quantitative trait loci underlyi ng appearance quality of rice grains (Oryza sativa ... L.). Yi ... Chuan Xue Bao 30, 251-259. ...

  2. Fluxes of diatoms in the Dona Paula Bay, west coast of India

    Digital Repository Service at National Institute of Oceanography (India)

    Garg, A.; Bhaskar, P.V.

    of POC and diatom carbon was 251 and 0.39 mg C m sup(-2) day sup(-1), respectively. The diatom carbon accounted for 0.15% of the POC flux. Mass flux of diatoms showed significant negative correlation with the concentration of nitrate and phosphate...

  3. Some Nonlinear Dynamic Inequalities on Time Scales

    Indian Academy of Sciences (India)

    Wei Nian Li; Weihong Sheng

    2007-11-01

    The aim of this paper is to investigate some nonlinear dynamic inequalities on time scales, which provide explicit bounds on unknown functions. The inequalities given here unify and extend some inequalities in (B G Pachpatte, On some new inequalities related to a certain inequality arising in the theory of differential equation, J. Math. Anal. Appl. 251 (2000) 736--751).

  4. AcEST: DK947591 [AcEST

    Lifescience Database Archive (English)

    Full Text Available Q+ + + A AEE + +R+ +DG CP+C Sbjct: 110 PTCAEADVVAGPRARERFHQLWSARAAAKAEESRRRRRRDDQAAAAGASGRLDGAACPVC 169 Query: 321 YEPMAA-GDGKPLEP... ++ I G DCPICY+ M Sbjct: 231 APLAPNDVTNPRLREAYDRALGRPSTSTAPAAAPESPTGKKRIPGPEDDCPICYDGM--- 287 Query: 342 DGKPLEP...F++++VL++ + +QKALL +EL + Sbjct: 251 NVYTVTIDKTPRCNCPDALKGNHCKHILFILIKVLQVSQVSGHWYQKALLTTELESIFAE 310 Query: ...SDASPNETQKSIQRDINGDCPVCYEAMAGDDNKP 258 Query: 354 LEPVVFCKTCGNNVHADCFKRWSQSKKH--GSVTCIYCRA...AGGEGAGEEAGGAAGKKGVQRPVEG 301 Query: 306 DCPICYEPMAAGDGKPLEPVVFCKTCGNNVHADCFKRWSQSKKHG--SVTCIYCRAIWV- 476 DC

  5. Effect of abandonment and plant classification on carbohydrate reserves of meadow plants

    Czech Academy of Sciences Publication Activity Database

    Janeček, Štěpán; Lanta, Vojtěch; Klimešová, Jitka; Doležal, Jiří

    2010-01-01

    Roč. 13, č. 2 (2010), s. 243-251. ISSN 1435-8603 R&D Projects: GA ČR GA526/09/0963; GA ČR(CZ) GA526/06/0723 Institutional research plan: CEZ:AV0Z60050516 Keywords : carbohydrates * meadow * abandonment Subject RIV: EF - Botanics Impact factor: 2.409, year: 2010

  6. Morphology and properties of SEBS block copolymer compatibilized PS/HDPE blends

    Czech Academy of Sciences Publication Activity Database

    Rek, V.; Vranješ, N.; Šlouf, Miroslav; Fortelný, Ivan; Jelčic, Ž.

    2008-01-01

    Roč. 40, č. 3 (2008), s. 237-251. ISSN 0095-2443 Grant ostatní: Ministry of Science, Education and Sport(HR) 0125059 Institutional research plan: CEZ:AV0Z40500505 Keywords : aPS/HDPE/SEBS blends * morphology * processing * rheological Subject RIV: CD - Macromolecular Chemistry Impact factor: 0.658, year: 2008

  7. Autophagy is preferred pathway of camptothecin-induced programmed cell death of v-myb-transformed monoblasts

    Czech Academy of Sciences Publication Activity Database

    Zahradníčková, Eva; Souček, Karel; Horváth, Viktor; Šmarda, Jan

    Budapest, 2005. P-251. [13th Euroconference on Apoptosis. 01.10.2005-04.10.2005, Budapest] R&D Projects: GA ČR(CZ) GA301/03/1055; GA MŠk(CZ) MSM0021622415 Institutional research plan: CEZ:AV0Z50040507 Keywords : autophagy * v-myb * BM2 cells Subject RIV: BO - Biophysics

  8. „Meine gedanken haben in dero Schätzbahren Zeilen einen gwichtigen Resonnanz erhalten...“. Josef Winterhalder d. J. und seine Korrespondenz

    Czech Academy of Sciences Publication Activity Database

    Valeš, Tomáš

    Langenargen - Brno : Museum Langenargen am Bodensee - Masarykova univerzita, 2009 - (Slavíček, L.), s. 235-251 ISBN 978-3-00-027324-7 Institutional research plan: CEZ:AV0Z80330511 Keywords : Josef Winterhalder the Younger * correspondence Subject RIV: AL - Art, Architecture, Cultural Heritage

  9. New cryptic species of the 'revolutum' group of Echinostoma (Digenea: Echinostomatidae) revealed by molecular and morphological data

    Czech Academy of Sciences Publication Activity Database

    Georgieva, Simona; Selbach, C.; Faltýnková, Anna; Soldánová, Miroslava; Sures, B.; Skirnisson, K.; Kostadinova, Aneta

    2013-01-01

    Roč. 6, č. 64 (2013). ISSN 1756-3305 R&D Projects: GA ČR GAP505/10/1562 Institutional support: RVO:60077344 Keywords : Radix auricularia * Radix peregra * Stagnicola palustris * Echinostoma * Cryptic species * Europe Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 3.251, year: 2013

  10. Nondisjunction in Favor of a Chromosome: The Mechanism of Rye B Chromosome Drive during Pollen Mitosis

    Czech Academy of Sciences Publication Activity Database

    Banaei-Moghaddam, A.M.; Schubert, V.; Kumke, K.; Weiβ, O.; Klemme, S.; Nagaki, K.; Macas, Jiří; González-Sánchez, M.; Heredia, V.; Gómez-Revilla, D.; González-García, M.; Vega, J.M.; Puertas, M.J.; Houben, A.

    2012-01-01

    Roč. 24, č. 10 (2012), s. 4124-4134. ISSN 1040-4651 Institutional research plan: CEZ:AV0Z50510513 Keywords : chromosomes * centromere * repeats Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 9.251, year: 2012

  11. Optimization, Application, and Interpretation of Lactate Dehydrogenase Measurements in Microwell Determination of Cell Number and Toxicity

    NARCIS (Netherlands)

    Wolterbeek, H.T.; Van der Meer, A.J.G.M.

    2005-01-01

    The lactate dehydrogenase (LDH) assay was addressed for its sensitivity, disturbances by foaming, and cell number and size. Cells were from a U-251 MG grade IV human glioblastoma brain tumor cell line used in 100-µl well volumes. Cells were counted by microscopy and Coulter counting; assays were LDH

  12. 75 FR 82066 - Change in Discount Rate for Water Resources Planning

    Science.gov (United States)

    2010-12-29

    ... with Section 80(a), Public Law 93-251 (88 Stat. 34) and 18 CFR 704.39, which: (1) specify that the rate... Bureau of Reclamation Change in Discount Rate for Water Resources Planning AGENCY: Bureau of Reclamation... Resources Development Act of 1974 require an annual determination of a discount rate for Federal...

  13. Methyl 2-(2-hydroxyacetamidobenzoate

    Directory of Open Access Journals (Sweden)

    Samina Alam

    2010-04-01

    Full Text Available The title compound, C10H11NO4, was formed from 4,1-benzoxazepine-2,5(1H,3H-dione and ammonia gas. Intramolecular hydrogen bonding is present between the amide N—H group and the carbonyl O atom of the ester group. The crystal structure features intermolecular O—H...O hydrogen bonds.

  14. Oil Price Hike Boosts CNOOC Revenue%Oil Price Hike Boosts CNOOC Revenue

    Institute of Scientific and Technical Information of China (English)

    2011-01-01

    Q1 production up 26.6 percent CNOOC Limited's first-quarter production increased 26.6 percent to 85.2 million barrels of oil equivalent compared with the same period last year. Output at the Jinzhou 25-1 project commenced in the first quarter, CNOOC sa

  15. Electron and hole traps in X-ray irradiated Y.sub.2./sub.SiO.sub.5./sub. and Lu.sub.2./sub.SiO.sub.5./sub. crystals

    Czech Academy of Sciences Publication Activity Database

    Kärner, T.; Laguta, Valentyn; Nikl, Martin; Zazubovich, S.

    2014-01-01

    Roč. 251, č. 4 (2014), s. 741-747. ISSN 0370-1972 R&D Projects: GA ČR GAP204/12/0805 Institutional support: RVO:68378271 Keywords : carrier trapping * oxyorthosilicates * thermally stimulated luminescence Subject RIV: BM - Solid Matter Physics ; Magnetism Impact factor: 1.489, year: 2014

  16. Stage-specific survival of epithelial cancers in North-Holland/Flevoland, The Netherlands

    NARCIS (Netherlands)

    Visser, O.; Leeuwen, van F.E.

    2005-01-01

    While stage is the most important factor for determining cancer survival, population-based survival data according to stage are rarely presented. We present such data for a large population diagnosed with cancer in the area covered by the Amsterdam Cancer Registry for the period 1989-2001 (n=108,251

  17. 78 FR 16036 - Service Level Environmental Impact Statement for the Texas Oklahoma Passenger Rail Study Corridor...

    Science.gov (United States)

    2013-03-13

    ... 64 FR 28545 dated May 26, 1999 (Environmental Procedures). The Service Level EIS will also address... Procedures (78 FR 2713; January 14, 2013). FRA and TxDOT will use a tiered process, as provided for in 40 CFR.... through 4 p.m. and from 6 p.m. through 8 p.m. Ardmore: Ardmore Train Station, 251 E. Main Street,...

  18. Partial Remission Definition

    DEFF Research Database (Denmark)

    Andersen, Marie Louise Max; Hougaard, Philip; Pörksen, Sven;

    2014-01-01

    OBJECTIVE: To validate the partial remission (PR) definition based on insulin dose-adjusted HbA1c (IDAA1c). SUBJECTS AND METHODS: The IDAA1c was developed using data in 251 children from the European Hvidoere cohort. For validation, 129 children from a Danish cohort were followed from the onset of...

  19. Circadian regulation of epithelial functions in the intestine

    Czech Academy of Sciences Publication Activity Database

    Pácha, Jiří; Sumová, Alena

    2013-01-01

    Roč. 208, č. 1 (2013), s. 11-24. ISSN 1748-1708 R&D Projects: GA ČR(CZ) GAP303/10/0969; GA ČR(CZ) GAP303/11/0668 Institutional support: RVO:67985823 Keywords : circadian rhythms * intestine * colon * proliferation * digestion * intestinal transport Subject RIV: ED - Physiology Impact factor: 4.251, year: 2013

  20. Occurence of fruit trees phytoplasmas in the Czech Republic

    Czech Academy of Sciences Publication Activity Database

    Navrátil, M.; Válová, P.; Fialová, R.; Fránová, Jana; Voráčková, Zdenka; Karešová, R.

    Bethesda : The International society for horticultural science, 1997, s. 166-167. [International symposium on virus and virus-like diseases of temperate fruit crops /17./. Bethesda (US), 23.06.1997-27.06.1997] R&D Projects: GA MŠk OK 251 Keywords : plant pathology * plant diseases

  1. Entheseal changes: Do they reflect socioeconomic status in the Early Medieval Central European population? (Mikulčice - Klášteřisko, Great Moravian Empire, 9th - 10th century)

    Czech Academy of Sciences Publication Activity Database

    Havelková, P.; Hladík, Marek; Velemínský, P.

    2013-01-01

    Roč. 23, č. 2 (2013), s. 237-251. ISSN 1047-482X Institutional research plan: CEZ:AV0Z80010507 Institutional support: RVO:68081758 Keywords : Early Middle Ages * enthesopathies * factor analysis * grave goods * musculoskeletal stress markers * social status Subject RIV: AC - Archeology, Anthropology, Ethnology Impact factor: 1.070, year: 2013

  2. 36 CFR 254.15 - Title standards.

    Science.gov (United States)

    2010-07-01

    ... subdivisions thereof (36 CFR part 251, subpart A). (iii) Any personal property owned by the non-Federal party... lands affected by a land exchange may be entitled to relocation benefits under 49 CFR 24.2. Unless otherwise provided by law or regulation (49 CFR 24.101(a)(1)), relocation benefits are not applicable...

  3. Percipovaná sociální opora dětí na základních školách

    Czech Academy of Sciences Publication Activity Database

    Kressa, Jiří

    Toruň: Akapit, 2013 - (Jędrzejko, M.), s. 251-262 ISBN 978-83-63955-18-2 R&D Projects: GA ČR(CZ) GAP407/12/2325 Institutional support : RVO:68081740 Keywords : social support * defenders * pupils * helping Subject RIV: AN - Psychology

  4. Psycholingvistika a čeština: některá slibná témata

    Czech Academy of Sciences Publication Activity Database

    Smolík, Filip

    2009-01-01

    Roč. 92, č. 5 (2009), s. 240-251. ISSN 0027-8203 R&D Projects: GA AV ČR KJB700250801 Institutional research plan: CEZ:AV0Z70250504 Keywords : psycholinguistics * sentence processing * word identification Subject RIV: AN - Psychology

  5. Disease: H01033 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available H01033 Congenital bilateral absence of vas deferens The condition of congenital bilateral absenc ... atic dysfunction, elevated sweat electrolytes, and male ... infertility. Mutations in the cystic fibrosis tran ... H, Cassiman JJ CFTR mutations and polymorphisms in male ... infertility. Int J Androl 27:251-6 (2004) PMID:914 ...

  6. Glaucoma Research Foundation

    Science.gov (United States)

    ... and research. Subscribe Browse the Gleams Archive » Glaucoma Research Foundation www.glaucoma.org 251 Post Street, Suite 600 San Francisco , CA 94108 (415) 986-3162 (800) 826-6693 question@ glaucoma.org ... Progress Get Involved News About Us Donate Informacion ...

  7. 24 CFR 207.259 - Insurance benefits.

    Science.gov (United States)

    2010-04-01

    ... insurance pursuant to 24 CFR parts 251, 252, or 255, the amount of the fee set forth through Federal... 24 Housing and Urban Development 2 2010-04-01 2010-04-01 false Insurance benefits. 207.259 Section... DEVELOPMENT MORTGAGE AND LOAN INSURANCE PROGRAMS UNDER NATIONAL HOUSING ACT AND OTHER AUTHORITIES...

  8. A new species of the Camponotus aureopilus VIEHMEYER, 1914 species-group (Hymenoptera: Formicidae) from Papua New Guinea

    Czech Academy of Sciences Publication Activity Database

    Shattuck, S.; Janda, Milan

    2009-01-01

    Roč. 12, - (2009), s. 251-253. ISSN 1994-4136 R&D Projects: GA MŠk LC06073; GA AV ČR KJB612230701 Grant ostatní: U.S. National Science Foundation(US) DEB-02-11591 Institutional research plan: CEZ:AV0Z50070508 Keywords : taxonomic description * Hymenoptera * Formicidae Subject RIV: EG - Zoology

  9. Two-year oscillation cycle in abundance of soybean aphid in Indiana

    Czech Academy of Sciences Publication Activity Database

    Rhainds, M.; Yoo, H. J. S.; Kindlmann, Pavel; Voegtlin, D.; Castillo, D.; Rutledge, C.; Sadof, C.; Yaninek, S.; O'Neil, R. J.

    2010-01-01

    Roč. 12, č. 3 (2010), s. 251-257. ISSN 1461-9555 Institutional research plan: CEZ:AV0Z60870520 Keywords : Aphididae * Aphis glycines * autumn migration * Hemiptera * heteroecy * Rhamnus * seesaw effect * soybean aphid Subject RIV: EH - Ecology, Behaviour Impact factor: 1.484, year : 2010

  10. Demolition work

    CERN Multimedia

    2004-01-01

    Because of demolition work inside Bldg. 251, two containers will be placed on the route Démocrite. For security reasons, the part of this road in front of barrack 553 will be closed to circulation from 5 to 12 November. Thank you, in advance for your understanding. PH-SMI

  11. University Student Reading Preferences in Relation to the Big Five Personality Dimensions

    Science.gov (United States)

    Schutte, Nicola S.; Malouff, John M.

    2004-01-01

    In this study 251 Australian university students, with a mean age of 26.46, indicated their preference for different types of reading material. A subset of 136 participants additionally reported how many hours per week they typically engage in recreational reading and non-recreational reading, indicated their motivation for recreational reading,…

  12. On the weak solution to the Oseen-type problem arising from flow around a rotating rigid body in the whole space

    Czech Academy of Sciences Publication Activity Database

    Kračmar, S.; Nečasová, Šárka; Penel, P.

    2006-01-01

    Roč. 5, č. 3 (2006), s. 243-251. ISSN 1109-2769 R&D Projects: GA AV ČR(CZ) IAA100190505 Institutional research plan: CEZ:AV0Z10190503 Keywords : rigid rotating body * weak solution * existence Subject RIV: BA - General Mathematics

  13. Factors predicting optic nerve axonal degeneration after methanol-induced acute optic neuropathy: a 2-year prospective study in 54 patients

    Czech Academy of Sciences Publication Activity Database

    Zakharov, S.; Nurieva, O.; Kotíková, K.; Urban, P.; Navrátil, Tomáš; Pelclová, D.

    2016-01-01

    Roč. 147, č. 1 (2016), s. 251-261. ISSN 0026-9247 Institutional support: RVO:61388955 Keywords : methanol optic neuropathy * visual evoked potentials * axonal degeneration Subject RIV: CF - Physical ; Theoretical Chemistry Impact factor: 1.222, year: 2014

  14. Career Education Needs of Secondary School Graduates from Asturias, Spain.

    Science.gov (United States)

    Gonzalez, Marisa Pereira

    1997-01-01

    A career education questionnaire completed by 1,251 11th graders, 1,137 12th graders, 371 vocational students, and 238 other high school students in Asturias, Spain, revealed limited knowledge of themselves and occupations, the need to improve job seeking/finding/getting skills, and few opportunities to participate in career exploration…

  15. Two- and three-beam interferometric optical tweezers

    Czech Academy of Sciences Publication Activity Database

    Casaburi, A.; Pesce, G.; Zemánek, Pavel; Sasso, A.

    2005-01-01

    Roč. 251, 4-6 (2005), s. 393-404. ISSN 0030-4018 R&D Projects: GA AV ČR(CZ) IAA1065203 Keywords : Optical force * Mie particle * Interferometric optical tweezers * Optical trapping Subject RIV: BH - Optics, Masers, Lasers Impact factor: 1.456, year: 2005

  16. Kovové artefakty

    Czech Academy of Sciences Publication Activity Database

    Žákovský, P.; Hošek, Jiří

    Brno: Archaia Brno, o.p.s, 2015 - (Plaček, M.; Dejmal, M.), s. 220-251 ISBN 978-80-905546-3-4 Grant ostatní: GA ČR(CZ) GAP405/11/1729 Institutional support: RVO:67985912 Keywords : castle * Middle Ages * iron objects * archaeometallurgy Subject RIV: AC - Archeology, Anthropology, Ethnology

  17. Fabrication of periodically ordered diamond nanostructures by microsphere lithography

    Czech Academy of Sciences Publication Activity Database

    Domonkos, Mária; Ižák, Tibor; Štolcová, L.; Proška, J.; Kromka, Alexander

    2014-01-01

    Roč. 251, č. 12 (2014), s. 2587-2592. ISSN 0370-1972 R&D Projects: GA ČR(CZ) GBP108/12/G108 Institutional support: RVO:68378271 Keywords : CVD growth * diamond * microsphere lithography * selective area deposition Subject RIV: BM - Solid Matter Physics ; Magnetism Impact factor: 1.489, year: 2014

  18. Sledování obsahu tritia ve srážkových vodách

    Czech Academy of Sciences Publication Activity Database

    Světlík, Ivo; Budská, E.

    2001-01-01

    Roč. 9, 7/8 (2001), s. 251-254. ISSN 1210-7085 R&D Projects: GA AV ČR KSK4055109 Institutional research plan: CEZ:AV0Z1048901 Keywords : tritium * monitoring * atmospheric precipitation Subject RIV: DL - Nuclear Waste, Radioactive Pollution ; Quality

  19. Rose Atoll Site 27P 6/21/2005 (45)M

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — One-meter-square (1 meter x 1 meter) benthic substrate at Rose Atoll, site 27P (14 33.038S, 168 09.251W), at meter 45 along a permanent transect.

  20. Management and use of dairy cattle feed resources on smallholder certified organic pineapple farms in Central Uganda

    DEFF Research Database (Denmark)

    Kiggundu, Muhammad; Kabi, Fred; Vaarst, Mette;

    2014-01-01

    major dairy cattle feed resources while only 19% reported using elephant grass. Banana peels (25.1%) and sweet potato vines (24.7%) were the most important crop residues fed to cattle. Farmers reported high cost of concentrates and scarcity of feeds as their biggest challenges in dairy cattle production...

  1. Statistical interpretation of WEBNET seismograms by artificial neural nets

    Czech Academy of Sciences Publication Activity Database

    Plešinger, Axel; Růžek, Bohuslav; Boušková, Alena

    2000-01-01

    Roč. 44, č. 2 (2000), s. 251-271. ISSN 0039-3169 R&D Projects: GA AV ČR IAA312104; GA ČR GA205/99/0907 Institutional research plan: CEZ:AV0Z3012916 Subject RIV: DC - Siesmology, Volcanology, Earth Structure Impact factor: 0.761, year: 2000

  2. Bolestná Panna Marie z Jirkova

    Czech Academy of Sciences Publication Activity Database

    Dáňová, Helena

    Chomutov : Oblastní muzeum v Chomutově, 2014 - (Dáňová, H.; Gubíková, R.), s. 251-255 ISBN 978-80-87898-07-9 Institutional support: RVO:68378033 Keywords : Our Lady of Sorrows * Jirkov * Josef Opitz Subject RIV: AL - Art, Architecture, Cultural Heritage

  3. The Constitutional Review Chamber of the Republic of Estonia : 3-4-1-5-01 decision of 3 March 2001 : review of the petition of Saare County Court to declare clause 25 (1) of Procedure for Privatisation of Land by Auction invalid

    Index Scriptorium Estoniae

    2001-01-01

    Riigikohtu lahendi 3-4-1-5-01 (Saare Maakohtu taotlus kontrollida Vabariigi Valitsuse 06. 11. 96. a. määrusega nr. 268 kinnitatud "Maa enampakkumisega erastamise korra" p. 25.1 vastavus seadusele ja põhiseadusele) tekst inglise keeles

  4. Long-term effects of CO2 enrichment on bud phenology and shoot growth patterns of Norway spruce juvenile trees

    Czech Academy of Sciences Publication Activity Database

    Pokorný, Radek; Tomášková, Ivana; Drápelová, I.; Kulhavý, J.; Marek, Michal V.

    2010-01-01

    Roč. 56, č. 6 (2010), s. 251-257. ISSN 1212-4834 R&D Projects: GA AV ČR IAA600870701 Institutional research plan: CEZ:AV0Z60870520 Keywords : bud * elevated CO2 * Norway spruce Subject RIV: EH - Ecology, Behaviour

  5. Vymezení a typologie postindustriální krajiny Česka

    Czech Academy of Sciences Publication Activity Database

    Kolejka, Jaromír; Klimánek, M.

    2012-01-01

    Roč. 117, č. 3 (2012), s. 289-307. ISSN 1212-0014 Institutional support: RVO:68145535 Keywords : typology * post-industrial landscape * PIL mapping * Czechia Subject RIV: DE - Earth Magnetism, Geodesy, Geography Impact factor: 0.500, year: 2012 http://geography.cz/sbornik/clanky-z-geografie-20123-ke-stazeni-251/

  6. Complete genome sequence of a novel circovirus from zebra finch.

    Science.gov (United States)

    Rinder, Monika; Schmitz, Anna; Peschel, Andrea; Korbel, Rüdiger

    2015-01-01

    A novel circovirus was identified in zebra finches (Taeniopygia guttata). The genome of the circovirus strain, designated 8454V25-1, comprised 1,982 nucleotides with two major open reading frames encoding a replication-associated protein and a viral capsid protein. PMID:26021933

  7. 41 CFR 109-25.109-2 - Equipment pools.

    Science.gov (United States)

    2010-07-01

    ...-GENERAL 25.1-General Policies § 109-25.109-2 Equipment pools. (a)-(c) (d) The report on the use and effectiveness of equipment pools shall be submitted to the head of the DOE office at the discretion of that... 41 Public Contracts and Property Management 3 2010-07-01 2010-07-01 false Equipment pools....

  8. Spectroscopy of Electro- and Photo-Productions of Hypernuclei

    Czech Academy of Sciences Publication Activity Database

    Motoba, T.; Bydžovský, Petr; Sotona, Miloslav; Itonaga, K.

    -, č. 185 (2010), s. 224-251. ISSN 0375-9687 Institutional research plan: CEZ:AV0Z10480505 Keywords : GAMMA-RAY SPECTROSCOPY * JLAB HALL-C * KAON PHOTOPRODUCTION Subject RIV: BE - Theoretical Physics Impact factor: 1.017, year: 2010

  9. EST Table: FS829564 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available FS829564 E_FL_fmgV_32F08_R_0 10/09/28 42 %/251 aa ref|NP_001116815.1| carboxylesterase CarE -11 [ ... Bombyx mori] gb|ACB12415.1| carboxylesterase CarE -11 [Bombyx mori] 10/09/10 30 %/227 aa FBpp0154663| ...

  10. High Charge-Carrier Mobility of 2.5 cm(2) V(-1) s(-1) from a Water-Borne Colloid of a Polymeric Semiconductor via Smart Surfactant Engineering.

    Science.gov (United States)

    Cho, Jangwhan; Cheon, Kwang Hee; Ahn, Hyungju; Park, Kwang Hun; Kwon, Soon-Ki; Kim, Yun-Hi; Chung, Dae Sung

    2015-10-01

    Semiconducting polymer nanoparticles dispersed in water are synthesized by a novel method utilizing non-ionic surfactants. By developing a smart surfactant engineering technique involving a selective post-removal process of surfactants, an unprecedentedly high mobility of 2.51 cm(2) V(-1) s(-1) from a water-borne colloid is demonstrated for the first time. PMID:26288123

  11. Flow Mechanisms in Creep of AZ91 Magnesium-based Composite

    Czech Academy of Sciences Publication Activity Database

    Sklenička, Václav; Pahutová, Marie; Kuchařová, Květa; Svoboda, Milan; Langdon, T. G.

    Mnichov: WileyVCH, 2000 - (Kainer, K.), s. 246-251 [Magnesium Alloys and their Application s. Mnichov (DE), 26.09.2000-28.09.2000] R&D Projects: GA AV ČR IAA2041902; GA AV ČR KSK1010601 Institutional research plan: CEZ:AV0Z2041904 Keywords : creep * magnesium alloys * composite Subject RIV: JG - Metallurgy

  12. The Relationship between Perceived Parenting Style and Hope in College Students.

    Science.gov (United States)

    Edwards, Lisa M.; Price, Gary E.

    The purpose of this study was to investigate the relationship between levels of hope and perceived parental authority style in college students (N=251). It was expected that there would be a positive relationship between authoritative parenting and students' levels of hope. It was also expected that there would be a negative relationship between…

  13. Longitudinal Predictors of Chinese Word Reading and Spelling among Elementary Grade Students

    Science.gov (United States)

    Yeung, Pui-Sze; Ho, Connie Suk-Han; Wong, Yau-Kai; Chung, Kevin Kien-Hoa; Lo, Lap-Yan

    2013-01-01

    The longitudinal predictive power of four important reading-related skills (phonological skills, rapid naming, orthographic skills, and morphological awareness) to Chinese word reading and writing to dictation (i.e., spelling) was examined in a 3-year longitudinal study among 251 Chinese elementary students. Rapid naming, orthographic skills, and…

  14. Simulation of Space Charge Effects in Electron Optical System Based on the Calculations of Current Density

    Czech Academy of Sciences Publication Activity Database

    Zelinka, Jiří; Oral, Martin; Radlička, Tomáš

    2015-01-01

    Roč. 21, S4 (2015), s. 246-251. ISSN 1431-9276 R&D Projects: GA MŠk(CZ) LO1212 Institutional support: RVO:68081731 Keywords : electron optical system * calculations of current density Subject RIV: JA - Electronics ; Optoelectronics, Electrical Engineering Impact factor: 1.877, year: 2014

  15. Integration of microalgae into biotechnologie for mitigation of carbon dioxide emissions

    Czech Academy of Sciences Publication Activity Database

    Doucha, Jiří; Straka, F.; Lívanský, Karel

    Delhi : Verlag, 2006, s. 250-251. [International Conference on Applied Phycology Algae in Biotechnology and Environment. Delhi (IN), 14.02.2006-15.02.2006] Institutional research plan: CEZ:AV0Z50200510 Keywords : microalgae * carbon dioxide Subject RIV: EE - Microbiology, Virology

  16. Silver salt of 4,6-diazido-N-nitro-1,3,5-triazine-2-amine - characterization of this primary explosive

    Czech Academy of Sciences Publication Activity Database

    Musil, T.; Matyáš, R.; Vala, R.; Růžička, A.; Vlček, Milan

    2014-01-01

    Roč. 39, č. 2 (2014), s. 251-259. ISSN 0721-3115 Institutional support: RVO:61389013 Keywords : primary explosive * AgDANT * silver salt of 4,6-diazido-N-nitro-1,3,5-triazine-2-amine Subject RIV: CC - Organic Chemistry Impact factor: 1.604, year: 2014

  17. Rudolf Winternitz

    Czech Academy of Sciences Publication Activity Database

    Šimůnek, Michal V.

    Červený Kostelec : Pavel Mervart, 2013 - (Šimůnek, M.; Kostlán, A.), s. 249-251 ISBN 978-80-7465-041-3. - (Studies in the History of Sciences and Humanities. 29) Institutional support: RVO:68378114 Keywords : holocaust * intellectual elite * history of science Subject RIV: AB - History

  18. Fanon, F. (1961), Les damnés de la terre, Paris, Editions Maspero. (Ressenya)

    OpenAIRE

    Velázquez Velázquez, Raquel

    2012-01-01

    Resenya de l'obra de Fanon, F. (1961), Les damnés de la terre, París, Éditions Maspero. Edición consultada: Los condenados de la tierra, Navarra, Txalaparta, 1999, 251 pp. Con Prefacio de Jean-Paul Sartre, fechado en setiembre de 1961.

  19. 41 CFR 101-25.104-1 - Redistribution, repair, or rehabilitation.

    Science.gov (United States)

    2010-07-01

    ..., or rehabilitation. 101-25.104-1 Section 101-25.104-1 Public Contracts and Property Management Federal...-GENERAL 25.1-General Policies § 101-25.104-1 Redistribution, repair, or rehabilitation. Prior to the... through redistribution, repair, or rehabilitation of already owned furniture and office machines....

  20. Opuštěné vojenské prostory jako významná refugia motýlí fauny

    Czech Academy of Sciences Publication Activity Database

    Vrba, Pavel; Čížek, Oldřich; Marhoul, P.; Zámečník, J.; Beneš, Jiří; Konvička, Martin

    2012-01-01

    Roč. 2012, č. 5 (2012), s. 251-254. ISSN 0044-4812 R&D Projects: GA ČR GAP505/10/2167 Grant ostatní: Ministerstvo životního prostředí(CZ) VaV/SP/2d3/153/08 Institutional support: RVO:60077344 Keywords : butterfly fauna