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Sample records for benzopyrans

  1. Bis-(1-2-benzopyran-1-one) derivatives: Synthesis and antimicrobial evaluation

    Indian Academy of Sciences (India)

    Poonam Kumar Koppula; Nalini Purohit

    2013-11-01

    The aim of the present study was to synthesize isocoumarin heterocycles and to elucidate the potential role of these compounds as biological active agents. A new series of isocoumarin derivatives containing two six-membered lactone rings is reported. 3-Aroyl-substituted isocoumarins (3) obtained by condensing 2-carboxy benzaldehyde (1) with bromoacetophenone derivatives (2) was further reacted with different aromatic aldehydes (4) affording bis-(1-2-benzopyran-1-one) derivatives (5). This short review compiles examples of most promising antibacterial, antifungal and analgesic bis-(1H-2-benzopyran-1-one) derivatives. The products were characterized on the basis of analytical and spectral (IR, 1HNMR, C13NMR, Mass) data. The biological activity study revealed that all compounds showed promising activities and bis-(1-2-benzopyran-1-one) derivatives (5) were found to be more active than 3-aroyl-substituted isocoumarins (3).

  2. Novel Synthesis of 2-Amino-3-ethoxycarbonyl Benzopyrans with Onium Salt as Soluble Support

    Institute of Scientific and Technical Information of China (English)

    ZHANG Li; LU Cui-fen; YANG Gui-chun; CHEN Zu-xing

    2011-01-01

    Task specific onium salt has been presented as soluble support in the synthesis of benzopyrans in this paper. This method allows for results by far superior to those reported other soluble supports and allows for very easy purifications of reaction products.

  3. Photochromism of indolino-benzopyrans studied by NMR and UV-visible spectroscopy

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    2006-01-01

    Full Text Available The synthesis of photochromic 3,3-di( 4 ′ -fluorophenyl-3H-benzopyrans fused to an indole moiety is described. The structures of photomerocyanines elucidated by NMR spectroscopy and spectrokinetic data ( λ max⁡ of colored form, colorability, and rate constant of bleaching obtained by UV-visible spectroscopy are reported.

  4. A precisely substituted benzopyran targets androgen refractory prostate cancer cells through selective modulation of estrogen receptors.

    Science.gov (United States)

    Kumar, Rajeev; Verma, Vikas; Sharma, Vikas; Jain, Ashish; Singh, Vishal; Sarswat, Amit; Maikhuri, Jagdamba P; Sharma, Vishnu L; Gupta, Gopal

    2015-03-15

    Dietary consumption of phytoestrogens like genistein has been linked with lower incidence of prostate cancer. The estradiol-like benzopyran core of genistein confers estrogen receptor-β (ER-β) selectivity that imparts weak anti-proliferative activity against prostate cancer cells. DL-2-[4-(2-piperidinoethoxy)phenyl]-3-phenyl-2H-1-benzopyran (BP), a SERM designed with benzopyran core, targeted androgen independent prostate cancer (PC-3) cells 14-times more potently than genistein, ~25% more efficiently than tamoxifen and 6.5-times more actively than ICI-182780, without forfeiting significant specificity in comparison to genistein. BP increased apoptosis (annexin-V and TUNEL labeling), arrested cell cycle, and significantly increased caspase-3 activity along with mRNA expressions of estrogen receptor (ER)-β and FasL (qPCR) in PC-3 cells. In classical ERE-luc reporter assay BP behaved as a potent ER-α antagonist and ER-β agonist. Accordingly, it decreased expression of ER-α target PS2 (P<0.01) and increased expression of ER-β target TNF-α (P<0.05) genes in PC-3. ER-β deficient PC-3 (siRNA-transfected) was resistant to apoptotic and anti-proliferative actions of SERMs, including stimulation of FasL expression by BP. BP significantly inhibited phosphorylation of Akt and ERK-1/2, JNK and p38 in PC-3 (immunoblotting), and thus adopted a multi-pathway mechanism to exert a more potent anti-proliferative activity against prostate cancer cells than natural and synthetic SERMs. Its precise ER-subtype specific activity presents a unique lead structure for further optimization.

  5. A precisely substituted benzopyran targets androgen refractory prostate cancer cells through selective modulation of estrogen receptors

    Energy Technology Data Exchange (ETDEWEB)

    Kumar, Rajeev; Verma, Vikas; Sharma, Vikas; Jain, Ashish; Singh, Vishal [Division of Endocrinology, CSIR—Central Drug Research Institute, Lucknow 226 031 (India); Sarswat, Amit [Division of Medicinal & Process Chemistry, CSIR—Central Drug Research Institute, Lucknow 226 031 (India); Maikhuri, Jagdamba P. [Division of Endocrinology, CSIR—Central Drug Research Institute, Lucknow 226 031 (India); Sharma, Vishnu L. [Division of Medicinal & Process Chemistry, CSIR—Central Drug Research Institute, Lucknow 226 031 (India); Gupta, Gopal, E-mail: g_gupta@cdri.res.in [Division of Endocrinology, CSIR—Central Drug Research Institute, Lucknow 226 031 (India)

    2015-03-15

    Dietary consumption of phytoestrogens like genistein has been linked with lower incidence of prostate cancer. The estradiol-like benzopyran core of genistein confers estrogen receptor-β (ER-β) selectivity that imparts weak anti-proliferative activity against prostate cancer cells. DL-2-[4-(2-piperidinoethoxy)phenyl]-3-phenyl-2H-1-benzopyran (BP), a SERM designed with benzopyran core, targeted androgen independent prostate cancer (PC-3) cells 14-times more potently than genistein, ~ 25% more efficiently than tamoxifen and 6.5-times more actively than ICI-182780, without forfeiting significant specificity in comparison to genistein. BP increased apoptosis (annexin-V and TUNEL labeling), arrested cell cycle, and significantly increased caspase-3 activity along with mRNA expressions of estrogen receptor (ER)-β and FasL (qPCR) in PC-3 cells. In classical ERE-luc reporter assay BP behaved as a potent ER-α antagonist and ER-β agonist. Accordingly, it decreased expression of ER-α target PS2 (P < 0.01) and increased expression of ER-β target TNF-α (P < 0.05) genes in PC-3. ER-β deficient PC-3 (siRNA-transfected) was resistant to apoptotic and anti-proliferative actions of SERMs, including stimulation of FasL expression by BP. BP significantly inhibited phosphorylation of Akt and ERK-1/2, JNK and p38 in PC-3 (immunoblotting), and thus adopted a multi-pathway mechanism to exert a more potent anti-proliferative activity against prostate cancer cells than natural and synthetic SERMs. Its precise ER-subtype specific activity presents a unique lead structure for further optimization. - Highlights: • BP with benzopyran core of genistein was identified for ER-β selective action. • BP was 14-times more potent than genistien in targeting prostate cancer cells. • It behaved as a potent ER-β agonist and ER-α antagonist in gene reporter assays. • BP's anti-proliferative action was inhibited significantly in ER-β deficient cells. • BP — a unique lead

  6. Two new benzopyran derivatives from Gouania leptostachya DC.var.tonkinensis Pitard.

    Institute of Scientific and Technical Information of China (English)

    Chong Yao; Shu Jie Zhang; Zheng Zhong Bai; Tong Zhou; Li Jiang Xuan

    2011-01-01

    Two new benzopyran derivatives, l-[(rel 25,3R)-3,5,7-trihydroxy-3,4-dihydro-2H-chromen-2-yl]ethanone (1), and l-[(rel 2S,35)-3,5,7-trihydroxy-3,4-dihydro-2H-chromen-2-yl]ethanone (2), along with four known flavonoids, prodelphinidin C, pro-delphinidin B3, (-)-epi-gallocatechin and (+)-gallocatechin, were isolated from the stems of Gouania leptostachya DC. var. tonkinensis Pitard. Their structures were determined on the basis of spectroscopic methods including HR-ESI-MS, ID and 2D NMR. Prodelphinidin C exhibited significant inhibitory activity for α-glucosidase.

  7. SYNTHESIS OF 6, 7-DISUBSTITUTED-2-ARYL-4H-1-BENZOPYRAN-4-ONES AS ANTIBACTERIAL AGENTS

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    Som Sukhen

    2012-03-01

    Full Text Available Benzopyrones constitute one of the major classes of natural products. Both semisynthetic and natural benzopyrone derivatives are known to possess important biological properties and as a result several attempts and procedures have been reported and developed for their synthesis. The present study was designed to synthesize various substituted 2-aryl-4H-1-benzopyran-4-one derivatives. The title compounds were prepared starting from a diketone i.e. 4-substituted-2-hydroxydiaroylmethane. The structures of these newly synthesized compounds were confirmed by their analytical and spectral data. The synthesized compounds were subjected for antibacterial screening against P.aeruginosa, B.subtilis and E.coli. Among all the derivatives 5d showed maximum activity against P.aeruginosa & E.coli and 5b showed highest activity against B.subtilis & E.coli. The results of the antibacterial screening suggest that some of the substituted 2-aryl-4H-1-benzopyran-4-one derivatives possess appreciable antibacterial activity and may prove useful in future drug development.

  8. 3,5,7-Trimethoxy-2-(4-methoxyphenyl-4H-1-benzopyran-4-one

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    Thammarat Aree

    2009-11-01

    Full Text Available In the title compound, C19H18O6, also known as 3,4′,5,7-tetramethoxyflavone, the dihedral angle between the benzopyran-4-one group and the attached benzene ring is 11.23 (8°. An intramolecular C—H...O hydrogen bond generates an S(6 ring motif. In the crystal, molecules are linked into a two-dimensional network parallel to (0overline{1}1 by intermolecular C—H...O hydrogen bonds, which generate R44(20, R44(12 and R22(14 ring motifs. Adjacent networks interact by π–π interactions between the pyran ring and its methoxyphenyl substituent [centroid–centroid distance = 3.5267 (8 Å].

  9. Benzopyrans as selective estrogen receptor beta agonists (SERBAs). Part 3: synthesis of cyclopentanone and cyclohexanone intermediates for C-ring modification.

    Science.gov (United States)

    Richardson, Timothy I; Dodge, Jeffrey A; Durst, Gregory L; Pfeifer, Lance A; Shah, Jikesh; Wang, Yong; Durbin, Jim D; Krishnan, Venkatesh; Norman, Bryan H

    2007-09-01

    Benzopyrans are selective estrogen receptor (ER) beta agonists (SERBAs), which bind the ER subtypes alpha and beta in opposite orientations. Here we describe the syntheses of cyclopentanone and cyclohexanone intermediates for SAR studies of the C-ring on the benzopyran scaffold. Modification of the C-ring disrupts binding to ERalpha, thus improving ERbeta selectivity up to 100-fold. X-ray cocrystal structures confirm previously observed binding modes.

  10. Polymer-supported palladacycles: efficient reagents for synthesis of benzopyrans with palladium recovery. Relationship among resin loading, Pd:P ratio, and reactivity of immobilized palladacycles.

    Science.gov (United States)

    Hershberger, John C; Zhang, Lei; Lu, Genliang; Malinakova, Helena C

    2006-01-06

    [reaction: see text] Oxapalladacycles were immobilized on polystyrene-divinylbenzene supports and treated with 3-aryl-2-propynoates or 1-alkyl-1,2-propadienes to afford 2H-1-benzopyrans in yields superior to those for solution-phase experiments. Isolation of benzopyrans was facilitated, and 71-80% of the palladium was recovered. Effects of resin loading with phosphorus and palladium were studied, and the optimum immobilized palladacycles featuring a medium loading with P (1.35 mmol P/g) and a high loading with Pd (Pd:P ratio 1:1.7) were identified. Resins with higher swelling capacities were more reactive.

  11. Synthesis, structure-activity relationships and brain uptake of a novel series of benzopyran inhibitors of insulin-regulated aminopeptidase.

    Science.gov (United States)

    Mountford, Simon J; Albiston, Anthony L; Charman, William N; Ng, Leelee; Holien, Jessica K; Parker, Michael W; Nicolazzo, Joseph A; Thompson, Philip E; Chai, Siew Yeen

    2014-02-27

    Peptide inhibitors of insulin-regulated aminopeptidase (IRAP) enhance fear avoidance and spatial memory and accelerate spatial learning in a number of memory paradigms. Using a virtual screening approach, a series of benzopyran compounds was identified that inhibited the catalytic activity of IRAP, ultimately resulting in the identification of potent and specific inhibitors. The present study describes the medicinal chemistry campaign that led to the development of the lead candidate, 3, highlighting the key structural features considered as critical for binding. Furthermore, the in vivo pharmacokinetics and brain uptake of compounds (1 and 3) were assessed in rats and were complemented with in vitro human and rat microsomal stability studies. Following intravenous administration to rodents, 3 exhibits brain exposure, albeit it is rapidly converted to 1, a compound which also exhibits potent inhibition of IRAP.

  12. Microwave-Assisted Improved Regioselective Synthesis of 12H-Benzopyrano[3,2-c][1]benzopyran-5-ones by Radical Cyclisation

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    Pradipta Kumar Basu

    2011-01-01

    Full Text Available Two new effective methodologies have been adopted for the preparation of 4-(2′-bromobenzyloxybenzopyran-7-ones 3(a–h. In the first methodology, 4-hydroxy[1]benzopyran-2-ones 1(a–d was alkylated with 2-bromobenzyl bromide 2a or 2-bromo-5-methoxy benzyl bromide 2b under phase transfer catalysis condition using lithium hydroxide/tetrabutyl ammonium bromide in N,N-dimethylformamide at 40–50°C and in the second method the microwave irradiation protocol has been exploited by simply mixing of 4-hydroxy[1]benzopyran-2-ones 1(a–d with 25% excess of 2-bromobenzyl bromide 2a or 2-bromo-5-methoxy benzyl bromide 2b. A catalytic amount of TBAB and potassium carbonate were added and irradiated in an open Erlenmeyer flask in a microwave oven for 4–10 min. The tributyltin-hydride-mediated radical cyclisation of 3(a–h was carried out under microwave irradiation to generate 12H-benzopyrano[3,2-c][1]benzopyran-5-ones 4(a–h in 78–88% yield and in this technique yields were significantly improved and reaction time was shortened compared to the previously reported conventional radical cyclisation method.

  13. Quantitative investigations of cation complexation of photochromic 8-benzothiazole-substituted benzopyran: towards metal-ion sensors.

    Science.gov (United States)

    Zakharova, Marianna I; Coudret, Christophe; Pimienta, Véronique; Micheau, Jean Claude; Delbaere, Stéphanie; Vermeersch, Gaston; Metelitsa, Anatoly V; Voloshin, Nikolai; Minkin, Vladimir I

    2010-02-01

    The photochromic, thermochromic and metallochromic behaviour of a series of three spiro[indoline-8-(benzothiazol-2-yl)-benzopyrans] has been investigated. The thermodynamic and kinetic parameters of their thermal equilibrium between the ring-closed (spiro) and ring-opened (merocyanine) isomeric forms have been determined using UV-Vis absorption and (1)H NMR spectroscopies. By adding Co(ii) and Ni(ii) ions in acetonitrile solution, 1 : 1 and 1 : 2 metal : merocyanine complexes are formed simultaneously. Using appropriate numerical methods, the kinetic analysis of the complexation allowed us to determine accurately key thermodynamic and spectroscopic parameters of the metal complexes. Results showed that the complexation strength is very sensitive to the size of the indoline nitrogen substituent. Complexation can be reversed by shining white light on the coloured complexes which regenerates the inactive spiropyran form, and releases the metallic ion; hence, these systems display fully reversible negative photochromism. The Zn(ii) complexes exhibit intense fluorescence in the 600-800 nm wavelength range. All these behaviours make these spiropyrans bearing benzothiazole heterocycles promising building blocks for the future construction of photodynamic chemosensors for transition metal ions.

  14. Polyketide and benzopyran compounds of an endophytic fungus isolated from Cinnamomum mollissimum:biological activity and structure

    Institute of Scientific and Technical Information of China (English)

    Carolina Santiago; Lin Sun; Murray Herbert Gibson Munro; Jacinta Santhanam

    2014-01-01

    Objective:To study bioactivity and compounds produced by an endophytic Phoma sp. fungus isolated from the medicinal plant Cinnamomum mollissimum. Methods: Compounds produced by the fungus were extracted from fungal broth culture with ethyl acetate. This was followed by bioactivity profiling of the crude extract fractions obtained via high performance liquid chromatography. The fractions were tested for cytotoxicity to P388 murine leukemic cells and antimicrobial activity against bacteria and pathogenic fungi. Compounds purified from active fractions which showed antibacterial, antifungal and cytotoxic activities were identified using capillary nuclear magnetic resonance analysis, mass spectrometry and admission to AntiMarin database. Results: Three known compounds, namely 4-hydroxymellein, 4,8-dihydroxy-6-methoxy-3-methyl-3,4-dihydro-1H-isochromen-1-one and 1-(2,6-dihydroxyphenyl) ethanone, were isolated from the fungus. The polyketide compound 4-hydroxymellein showed high inhibitory activity against P388 murine leukemic cells (94.6%) and the bacteria Bacillus subtilis (97.3%). Meanwhile, 4,8-dihydroxy-6-methoxy-3-methyl-3,4-dihydro-1H-isochromen-1-one, a benzopyran compound, demonstrated moderate inhibitory activity against P388 murine leukemic cells (48.8%) and the fungus Aspergillus niger (56.1%). The second polyketide compound, 1 (2,6-dihydroxyphenyl) ethanone was inactive against the tested targets. Conclusions: These findings demonstrate the potential of endophytes as producers of pharmacologically important compounds, including polyketides which are major secondary metabolites in fungi.

  15. Asymmetric Synthesis of Spirocyclic 2-Benzopyrans for Positron Emission Tomography of σ1 Receptors in the Brain

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    Katharina Holl

    2014-01-01

    Full Text Available Sharpless asymmetric dihydroxylation of styrene derivative 6 afforded chiral triols (R-7 and (S-7, which were cyclized with tosyl chloride in the presence of Bu2SnO to provide 2-benzopyrans (R-4 and (S-4 with high regioselectivity. The additional hydroxy moiety in the 4-position was exploited for the introduction of various substituents. Williamson ether synthesis and replacement of the Boc protective group with a benzyl moiety led to potent σ1 ligands with high σ1/σ2-selectivity. With exception of the ethoxy derivative 16, the (R-configured enantiomers represent eutomers with eudismic ratios of up to 29 for the ester (R-18. The methyl ether (R-15 represents the most potent σ1 ligand of this series of compounds, with a Ki value of 1.2 nM and an eudismic ratio of 7. Tosylate (R-21 was used as precursor for the radiosynthesis of [18F]-(R-20, which was available by nucleophilic substitution with K[18F]F K222 carbonate complex. The radiochemical yield of [18F]-(R-20 was 18%–20%, the radiochemical purity greater than 97% and the specific radioactivity 175–300 GBq/µmol. Although radiometabolites were detected in plasma, urine and liver samples, radiometabolites were not found in brain samples. After 30 min, the uptake of the radiotracer in the brain was 3.4% of injected dose per gram of tissue and could be reduced by coadministration of the σ1 antagonist haloperidol. [18F]-(R-20 was able to label those regions of the brain, which were reported to have high density of σ1 receptors.

  16. polyketide and benzopyran compounds of an endophytic fungus isolated from Cinnamomum mollissimum:biological activity and structure

    Institute of Scientific and Technical Information of China (English)

    Carolina; Santiago; Lin; Sun; Murray; Herbert; Gibson; Munro; Jacinta; Santhanam

    2014-01-01

    Objective:To study bioactivity and compounds produced by an endophytic Phoma sp.fungus isolated from the medicinal plant Cinnamomum mollissimum.Methods:Compounds produced by the fungus were extracted from fungal broth culture with ethyl acetate.This was followed by hioaclivity profiling of the crude extract fractions obtained via high performance liquid chromatography.The fractions were tested for cytotoxicity to P388 murine leukemic cells and antimicrobial activity against bacteria and pathogenic fungi.Compounds purified from active fractions which showed antibacterial,antifungal and cytotoxic activities were identified using capillary nuclear magnetic resonance analysis,mass spectrometry and admission to AntiMarin database.Results:Three known compounds,namely 4—hydroxymellein,4,8—dihydroxy—6—melhoxy—3—methyl—3,4-dihydro—1H-isochromen-1—one and 1—(2,6-dihydroxyphenyl) ethanone,were isolated from the fungus.The polyketide compound 4—hydroxymellein showed high inhibitory activity against P388 murine leukemic cells(94.6%) and the bacteria Bacillus sublilis(97.3%).Meanwhile.4,8—dihydroxy-6—melhoxy—3—meth) 1—3,4-dihydro—1H—isochromen—1-one,a benzopyran compound,demonstrated moderate inhibitory activity against P388 murine leukemic cells(48.8%)and the fungus Aspergillus niger(56.1%).The second polyketide compound.1(2,6—dihydroxyphenyl)ethanone was inactive against the tested targets.Conclusions:These findings demonstrate the potential of endophytes as producers ol pharmacologically important compounds,including polyketides which are major secondary metabolites in fungi.

  17. In Vitro evaluation of antifungal activity of Bioactive Compound 2H-FURO [2,3-H]-1-Benzopyran-2-one against seed borne fungi of maize

    Directory of Open Access Journals (Sweden)

    B. Kiran

    2012-03-01

    Full Text Available Antifungal activity of bioactive compound 2HFuro[ 2,3-H]-1-benzopyran-2-one recorded a significant activity at 100-1000 ppm concentration against all the ten Aspergillus species tested. A. flavus recorded complete inhibition at 100 ppm concentration, A. niger at 500 ppm, A. fumigates at 600 ppm, A. flavus oryzae and A. flavus columnaris at 700 ppm respectively. A. ochraceous and A. flavipes recorded complete inhibition at 900 ppm concentration. Compared to synthetic fungicide Captan and Thiram at 2000ppm concentration. Minimum Inhibitory Concentration (MIC of bioactive compound was in the range of 100- 900ppm concentration against all the test fungi.

  18. Evaluation of Antifungal Activity and Mode of Action of New Coumarin Derivative, 7-Hydroxy-6-nitro-2H-1-benzopyran-2-one, against Aspergillus spp.

    Science.gov (United States)

    Guerra, Felipe Queiroga Sarmento; de Araújo, Rodrigo Santos Aquino; de Sousa, Janiere Pereira; Pereira, Fillipe de Oliveira; Mendonça-Junior, Francisco J B; Barbosa-Filho, José M; de Oliveira Lima, Edeltrudes

    2015-01-01

    Aspergillus spp. produce a wide variety of diseases. For the treatment of such infections, the azoles and Amphotericin B are used in various formulations. The treatment of fungal diseases is often ineffective, because of increases in azole resistance and their several associated adverse effects. To overcome these problems, natural products and their derivatives are interesting alternatives. The aim of this study was to examine the effects of coumarin derivative, 7-hydroxy-6-nitro-2H-1-benzopyran-2-one (Cou-NO2), both alone and with antifungal drugs. Its mode of action against Aspergillus spp. Cou-NO2 was tested to evaluate its effects on mycelia growth and germination of fungal conidia of Aspergillus spp. We also investigated possible Cou-NO2 action on cell walls (0.8 M sorbitol) and on Cou-NO2 to ergosterol binding in the cell membrane. The study shows that Cou-NO2 is capable of inhibiting both the mycelia growth and germination of conidia for the species tested, and that its action affects the structure of the fungal cell wall. At subinhibitory concentration, Cou-NO2 enhanced the in vitro effects of azoles. Moreover, in combination with azoles (voriconazole and itraconazole) Cou-NO2 displays an additive effect. Thus, our study supports the use of coumarin derivative 7-hydroxy-6-nitro-2H-1-benzopyran-2-one as an antifungal agent against Aspergillus species.

  19. Evaluation of Antifungal Activity and Mode of Action of New Coumarin Derivative, 7-Hydroxy-6-nitro-2H-1-benzopyran-2-one, against Aspergillus spp.

    Directory of Open Access Journals (Sweden)

    Felipe Queiroga Sarmento Guerra

    2015-01-01

    Full Text Available Aspergillus spp. produce a wide variety of diseases. For the treatment of such infections, the azoles and Amphotericin B are used in various formulations. The treatment of fungal diseases is often ineffective, because of increases in azole resistance and their several associated adverse effects. To overcome these problems, natural products and their derivatives are interesting alternatives. The aim of this study was to examine the effects of coumarin derivative, 7-hydroxy-6-nitro-2H-1-benzopyran-2-one (Cou-NO2, both alone and with antifungal drugs. Its mode of action against Aspergillus spp. Cou-NO2 was tested to evaluate its effects on mycelia growth and germination of fungal conidia of Aspergillus spp. We also investigated possible Cou-NO2 action on cell walls (0.8 M sorbitol and on Cou-NO2 to ergosterol binding in the cell membrane. The study shows that Cou-NO2 is capable of inhibiting both the mycelia growth and germination of conidia for the species tested, and that its action affects the structure of the fungal cell wall. At subinhibitory concentration, Cou-NO2 enhanced the in vitro effects of azoles. Moreover, in combination with azoles (voriconazole and itraconazole Cou-NO2 displays an additive effect. Thus, our study supports the use of coumarin derivative 7-hydroxy-6-nitro-2H-1-benzopyran-2-one as an antifungal agent against Aspergillus species.

  20. Computer programs for calculating pKa: a comparative study for 3-(3-(2-nitrophenylprop-2-enoyl-2H-1-benzopyran-2-one

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    SELMA ŠPIRTOVIĆ-HALILOVIĆ

    2010-02-01

    Full Text Available Coumarin-based compounds containing a chalcone moiety exhibit antimicrobial activity. These substances are potential drugs and it is important to determine their pKa values. However, they are almost insoluble in water. The dissociation constant was experimentally determined by potentiometric titration for 3-[3-(2-nitrophenylprop-2-enoyl]-2H-1-benzopyran-2-one because this compound shows good activity and solubility. A number of different computer programs for the calculation of the dissociation constant of chemical compounds have been developed. The pKa value of the target compound was calculated using three different computer programs, i.e., the ACD/pKa, CSpKaPredictor and ADME/ToxWEB programs, which are based on different theoretical approaches. The analysis demonstrated good agreement between the experimentally observed pKa value of 3-[3-(2-nitrophenylprop-2-enoyl]-2H-1-benzopyran-2-one and the value calculated using the computer program CSpKa.

  1. Identification of Two Novel Anti-Fibrotic Benzopyran Compounds Produced by Engineered Strains Derived from Streptomyces xiamenensis M1-94P that Originated from Deep-Sea Sediments

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    Lei Feng

    2013-10-01

    Full Text Available The benzopyran compound obtained by cultivating a mangrove-derived strain, Streptomyces xiamenensis strain 318, shows multiple biological effects, including anti-fibrotic and anti-hypertrophic scar properties. To increase the diversity in the structures of the available benzopyrans, by means of biosynthesis, the strain was screened for spontaneous rifampicin resistance (Rif, and a mutated rpsL gene to confer streptomycin resistance (Str, was introduced into the S. xiamenensis strain M1-94P that originated from deep-sea sediments. Two new benzopyran derivatives, named xiamenmycin C (1 and D (2, were isolated from the crude extracts of a selected Str-Rif double mutant (M6 of M1-94P. The structures of 1 and 2 were identified by analyzing extensive spectroscopic data. Compounds 1 and 2 both inhibit the proliferation of human lung fibroblasts (WI26, and 1 exhibits better anti-fibrotic activity than xiamenmycin. Our study presents the novel bioactive compounds isolated from S. xiamenensis mutant strain M6 constructed by ribosome engineering, which could be a useful approach in the discovery of new anti-fibrotic compounds.

  2. Preparing photochromic nanofibers and animal cells using a photochromic compound of 1',3',3'-trimethyl-6-nitrospiro (2H-1-benzopyran-2,2'-indoline).

    Science.gov (United States)

    Li, Xiaoqiang; Lin, Lin; Kanjwal, Muzafar A; Chronakis, Ioannis S; Liu, Shuiping; Chen, Yanmo

    2012-01-01

    In this work, the photochromic compound 1',3',3'-trimethyl-6-nitrospiro (2H-1-benzopyran-2,2'-indoline) (NOSP) was synthesized by a two step process. The photochromic properties of NOSP were investigated by ultraviolet-visible (UV-Vis) spectrophotometry. The results showed that NOSP was very sensitive to UV irradiation with absorption peaks at about 336 nm and 567 nm. Our hypothesis was that both photochromic nanofibers and photochromic living animal cells could be obtained by combining them with NOSP. To test the hypothesis, photochromic nanofibers were fabricated by electrospinning from various mixed solutions of NOSP and polymers (including a synthetic polymer of poly(methyl methacrylate) and a natural polymer of gelatin); NOSP/ethanol solution was dissolved in culture medium to stain pig iliac endothelial cells (PIEC) and endow them with photochromic capability. Polymer nanofibers from electrospinning were characterized by water contact angle measurements, ultraviolet-visible (UV-Vis) spectrophotometry and fluorescence microscopy. Morphology of photochromic PIEC was observed by fluorescence microscopy after being irradiated. It was shown that nanofibers from electrospun polymers and NOSP-treated PIEC had photochromic properties. The bio-toxicity of the photochromic compound was also evaluated and it was shown that ~50% of PIEC remained viable for at least 20 min. The photochromic compound NOSP could be a potentially powerful tool for development of multi-functional nanofibers and biological applications.

  3. Effect of solvent environment on the Photophysics of a newly synthesized bioactive 7-oxy(5-selenocyanato-pentyl)-2H-1-benzopyran-2-one

    Energy Technology Data Exchange (ETDEWEB)

    Dhar, Sayaree [Department of Chemistry, Jadavpur University, Kolkata 700032 (India); Rana, Dipak Kumar [Department of Chemistry, Saldiha College, The University of Burdwan, Bankura 722173 (India); Singha Roy, Somnath [Department of Cancer Chemoprevention, Chittaranjan National Cancer Institute, 37, S. P. Mukherjee Road, Kolkata 700026 (India); Roy, Swapnadip [Department of Chemistry, Jadavpur University, Kolkata 700032 (India); Bhattacharya, Sudin [Department of Cancer Chemoprevention, Chittaranjan National Cancer Institute, 37, S. P. Mukherjee Road, Kolkata 700026 (India); Bhattacharya, Subhash Chandra, E-mail: sbjuchem@yahoo.com [Department of Chemistry, Jadavpur University, Kolkata 700032 (India)

    2012-04-15

    The synthesis and solvatochromic behavior of the compound, 7-oxy(5-selenocyanato-pentyl)-2H-1-benzopyran-2-one (PCM), a pervasive, bioactive coumarin derivative, which is expected to possess antioxidative properties and other important therapeutic activities of significant potency with low systematic toxicity, have been reported employing steady state and time resolved fluorescence techniques. Spectroscopic studies reveal that the solvatochromic behavior of the probe depends not only on the polarity of the medium but also on the hydrogen bonding properties of the solvents. Specific hydrogen bonding interaction of PCM in polar solvents modulated the order of the two close-lying lowest singlet states. The photophysical response of PCM in different solvents has been explained considering solute-solvent interactions. To corroborate these results, we performed the ground-state geometry, lowest energy transition and the UV-vis spectrum of PCM using the density functional theory (DFT) and the time-dependent density functional theory (TD-DFT) at B3LYP/6-31G{sup Low-Asterisk} level. We found excellent correlation between the predicted and experimental spectral data, providing a useful tool in the design of new fluorogenic probes having potential therapeutic activity. - Highlights: Black-Right-Pointing-Pointer Synthesis and photophysical behavior of the bioactive PCM. Black-Right-Pointing-Pointer The order of the two close-lying lowest singlet states is modulated by H-bonding interaction in polar solvents. Black-Right-Pointing-Pointer In non-polar solvent non-radiative path favor due to mixing of n,{pi}{sup Low-Asterisk} and {pi},{pi}{sup Low-Asterisk} singlet states. Black-Right-Pointing-Pointer Quantum chemical calculation has been employed to corroborate with experimental finding.

  4. Synthesis, structure, spectroscopic properties and DFT studies on some 7-hydroxy-4-methyl-8-(arylazo)-2H-1-benzopyran-2-one and their complexes with some divalent transition metal ions

    Science.gov (United States)

    Abdel-Latif, Samir A.; Mohamed, Adel A.

    2017-04-01

    A novel 7-hydroxy-4-methyl-8-(p-tolylazo)-2H-1-benzopyran-2-one (coumarin) (L1) and 7-hydroxy-4-methyl-8-(p-anisylazo)-2H-1-benzopyran-2-one (coumarin) (L2) and their metal complexes with Mn(II), Co(II), Ni(II) Cu(II) and Zn(II) have been prepared and characterized by elemental analysis, infrared (IR), proton nuclear magnetic resonance (1H NMR) and mass spectra. The solid complexes have been also characterized by thermal analyses (TG and DTA), magnetic measurements, electronic transition, molar conductance; mass spectra, and electron spin resonance (ESR). The molecular orbital calculations of the complexes have been performed using the density functional theory (DFT) method and the basis sets 6-31G* and 6-311G**. The computational results revealed that the proposed geometrical structures for the investigated metal complexes suggest trigonal bipyramid for 1:1 and tetrahedral geometry for 1:2 complexes. The 1:1 complexes contain coordinated and lattice held water molecules whereas 1:2 complexes contain only lattice water molecules. The complexes behave as non-electrolytes in dimethyl formamide (DMF).

  5. 5-Methylspiro[indoline-3,7′-[6H,7H,8H]pyrano[3,2-c:5,6-c′]di[1]benzopyran]-2,6′,8′-trione chloroform hemisolvate

    Directory of Open Access Journals (Sweden)

    Abdulrahman I. Almansour

    2012-04-01

    Full Text Available In the title compound, C27H15NO6·0.5CHCl3, the central pyran ring and both the benzopyran systems are planar, with the dihedral angle between the outer rings being 3.24 (6°. The indolin-2-one system is in a perpendicular configuration with respect to the pyran ring [dihedral angle = 87.58 (2°]. Supramolecular layers in the ac plane are formed in the crystal structure whereby inversion-related molecules are connected by N—H...O hydrogen bonds. These are further linked by C—H...O interactions, forming a supramolecular layer in the ac plane. Disordered CHCl3 solvent in the structure was modelled with the SQUEEZE routine in PLATON [Spek (2009. Acta Cryst. D65, 148–155].

  6. A New Approach for Studying Bond Rupture/Closure of a Spiro Benzopyran Photochromic Material: Reactivity Descriptors Derived from Frontier Orbitals and DFT Computed Electrostatic Potential Energy Surface Maps

    Directory of Open Access Journals (Sweden)

    M. S. A. Abdel-Mottaleb

    2016-01-01

    Full Text Available This paper focuses on computations technique within the framework of the TD-DFT theory for studying the relationship between structure-properties of reversible conversion of photochromic materials. Specifically, we report on 1′,3′-dihydro-8-methoxy-1′,3′,3′-trimethyl-6-nitrospiro[2H-1-benzopyran-2,2′-(2H-indole] (SP and its isomers. TD-DFT calculated UV-Vis electronic spectra of the closed and open isomers of this photochromic material are in excellent agreement with the experimental results. Moreover, this paper reports on the results of theoretical investigations of reactivity indices that may govern the conversion between spiropyrans and its isomers. In addition, the solvent and rigidity of the medium significantly control the thermal bleaching of the photogenerated colored isomers and hence the switch ability pattern of the photochromic material. The effect of molecular structure computed by DFT in gas-phase and solvents on Cspiro-O bond length has been shown to correlate with photochromic properties. For this compound, DFT optimized geometry could be used to predict photochromism. Furthermore, in an attempt to predict the driving force for MC → SP, this work explores, for the first time, profitable exploitation of the calculated and visualized mapped electrostatic potential energy surfaces (ESP map. Interestingly, it seems that the electrostatic potential forces over the molecular fragments govern spirobond rupture/closure reactions. Thermodynamically, all-trans-colored isomer (CTT is the most stable merocyanine-like form.

  7. Biological Properties Of Benzopyran-Based Platinum (Ii Complexes

    Directory of Open Access Journals (Sweden)

    Malinowska Katarzyna

    2014-04-01

    Full Text Available The aim of the study was to analyze the physicochemical synthesized complex 3 [(1,3- thiazol -2- ylimino methyl]-4H- chromene -4 -one with tetrachloroplatinate(II dipotassium and determination peroxidase activity and glutathione (GPX in red blood cells of cancer patients and healthy subjects. Materials and methods. Tests were carried out with the approval of the Bioethics Committee No. RNN/260/08/KB. Blood was collected into tubes with anticoagulant (heparin lithium. Determination of glutathione peroxidase activity was performed by methods of Little and O’Brien in 20 person groups hospitalized at the Department of General and Colorectal Surgery Veterans General Hospital in Łódź. Results. The study was an increase of activity in the control without the compound and after the introduction of the complex relative to the treatment groups. In healthy subjects, without the use of glutathione peroxidase complex averaged 73.25 ± 23.88 U / g Hb after application of the compound corresponds to the reference group 81.01 ± 25.94 U / g Hb. In contrast, in patients without the use of the complex activity amounted to 42.85 ± 27.49 U / g Hb. In the study group, which uses synthesized complex GPX activity corresponds to 67.72 ± 13.44 U / g Hb. Conclusions. The obtained results underline that the introduction of significant blood antioxidant complex research has a significant impact on the results of the determinations. Statistically significant (p < 0.05 difference occurred in both test and no relation to the administration of the complex in relation to the control of 1. 2.

  8. Microplate assay for screening the antibacterial activity of Schiff bases derived from substituted benzopyran-4-one.

    Science.gov (United States)

    Amin, Rehab M; Abdel-Kader, Nora S; El-Ansary, Aida L

    2012-09-01

    Schiff bases (SB(1)-SB(3)) were synthesized from the condensation of 6-formyl-7-hydroxy-5-methoxy-2-methylbenzopyran-4-one with 2-aminopyridine (SB(1)), p-phenylenediamine (SB(2)) and o-phenylenediamine (SB(3)), while Schiff bases (SB(4)-SB(6)) were synthesized by condensation of 5,7-dihydroxy-6-formyl-2-methylbenzopyran-4-one with 2-aminopyridine (SB(4)), p-phenylenediamine (SB(5)) and o-phenylenediamine (SB(6)). Schiff bases were characterized using elemental analysis, IR, UV-Vis, (1)H NMR, (13)C NMR and mass spectroscopy. These compounds were screened for antibacterial activities by micro-plate assay technique. Escherichia coli and Staphylococcus capitis were exposed to different concentrations of the Schiff bases. Results showed that the antibacterial effect of these Schiff bases on Gram-negative bacteria were higher than that on Gram-positive bacteria moreover, the Schiff bases containing substituent OCH(3) on position five have higher antibacterial activity than that containing hydroxy group on the same position.

  9. KR-31831, benzopyran derivative, inhibits VEGF-induced angiogenesis of HUVECs through suppressing KDR expression.

    Science.gov (United States)

    Park, Shi-Young; Seo, Eun-Hee; Song, Hyun Seok; Jung, Seung-Youn; Lee, Young-Kyoung; Yi, Kyu-Yang; Yoo, Sung-Eun; Kim, Yung-Jin

    2008-06-01

    Angiogenesis is important in the development and progression of cancer, therefore the therapeutic approach based on anti-angiogenesis may represent a promising therapeutic option. KR-31831 is a novel anti-ischemic agent. Previously, we reported the anti-angiogenic activity of KR-31831. In the present study we investigated the molecular mechanisms underlying anti-angiogenic activity of KR-31831. We show that KR-31831 inhibits vascular endothelial growth factor (VEGF)-induced proliferation and tube formation via release of intracellular Ca2+ and phosphorylation of extra-cellular regulated kinase 1/2 (Erk 1/2) in human umbilical vein endothelial cells (HUVECs). Moreover, the expression of VEGF receptor 2 (VEGFR2, known as Flk-1 or KDR) was reduced by the treatment of KR-31831. These results suggest that KR-31831 may have inhibitory effects on tumor angiogenesis through down-regulation of KDR expression.

  10. Photochromism of indolino-benzopyrans studied by NMR and UV-visible spectroscopy

    Directory of Open Access Journals (Sweden)

    S. Delbaere

    2006-01-01

    is described. The structures of photomerocyanines elucidated by NMR spectroscopy and spectrokinetic data (λmax⁡ of colored form, colorability, and rate constant of bleaching obtained by UV-visible spectroscopy are reported.

  11. Synthesis, spectral, thermal and magnetic studies of Mn(II), Ni(II) and Cu(II) complexes with some benzopyran-4-one Schiff bases.

    Science.gov (United States)

    el-Ansary, Aida L; Abdel-Fattah, Hussein M; Abdel-Kader, Nora S

    2011-08-01

    The Schiff bases of N(2)O(2) dibasic ligands, H(2)La and H(2)Lb are prepared by the condensation of ethylenediamine (a) and trimethylenediamine (b) with 6-formyl-7-hydroxy-5-methoxy-2-methylbenzopyran-4-one. Also tetra basic ligands, H(4)La and H(4)Lb are prepared by the condensation of aliphatic amines (a) and (b) with 6-formyl-5,7-dihydroxy-2-methylbenzopyran-4-one. New complexes of H(4)La and H(4)Lb with metal ions Mn(II), Ni(II) and Cu(II) are synthesized, in addition Mn(II) complexes with ligands H(2)La and H(2)Lb are also synthesized. Elemental and thermal analyses, infrared, ultraviolet-visible as well as conductivity and magnetic susceptibility measurements are used to elucidate the structure of the newly prepared metal complexes. The structures of copper(II) complexes are also assigned based upon ESR spectra study. All the complexes separated with the stoichiometric ratio (1:1) (M:L) except Mn-H(4)La and Mn-H(4)Lb with (2:1) (M:L) molar ratio. In metal chelates of the type 1:1 (M:L), the Schiff bases behave as a dinegative N(2)O(2) tetradentate ligands. Moreover in 2:1 (M:L) complexes, the Schiff base molecules act as mono negative bidentate ligand and binuclear complex is then formed. The Schiff bases were assayed by the disc diffusion method for antibacterial activity against Staphylococcus aureus and Escherichia coli. The antifungal activity of the Schiff bases was also evaluated against the fungi Aspergillus flavus and Candida albicans.

  12. 4-(4-Chlorophenyl-7,7-dimethyl-7,8-dihydro-4H-1-benzopyran-2,5(3H,6H-dione

    Directory of Open Access Journals (Sweden)

    Hao Shi

    2010-01-01

    Full Text Available The title compound, C17H17ClO3, has been synthesized by the reaction of p-chlorobenzaldehyde, isopropylidene malonate and 5,5-dimethylcyclohexane-1,3-dione with triethylbenzylammonium chloride in water as a green solvent. The six membered pyranone ring of the hexahydrocoumarin system has a screw-boat conformation while the dimethylcyclohexenone system has a distorted envelope conformation. The dihedral angle between the least-squares planes of the coumarin ring system and the benzene ring is 85.64 (9°.

  13. Synthesis, Characterization of La(III, Nd(III, and Er(III Complexes with Schiff Bases Derived from Benzopyran-4-one and Thier Fluorescence Study

    Directory of Open Access Journals (Sweden)

    Aida L. El-Ansary

    2012-01-01

    Full Text Available The Schiff bases, L1, L2, and L3, are synthesized from the condensation of 5,7-dihydroxy-6-formyl-2-methylbenzopyran-4-one (L with 2-aminopyridine (1, p-phenylenediamine (2, and o-phenylenediamine (3. The prepared Schiff bases react with lanthanum (III, neodymium (III, and erbium (III nitrate to give complexes with stoichiometric ratio (1 : 1 (ligand : metal. The binuclear complexes of Er(III with L3 and the three metal ions with L2 are separated. The complexes have been characterized by elemental analysis, molar conductance, electronic absorption, and infrared, 1H-NMR spectral studies. The presence of hydrated and coordinated water molecules is inferred from thermogravimetric analysis. Thermal degradation studies show that the final product is the metal oxide. The luminescence properties of the Nd(III and Er(III complexes in dimethylformamide (DMF solutions were investigated.

  14. Racemic tricarbonyl[(4a,5,6,7,8,8a-η-2-phenyl-3,4-dihydro-2H-1-benzopyran]chromium(0

    Directory of Open Access Journals (Sweden)

    Johannes H. van Tonder

    2010-09-01

    Full Text Available The title compound, [Cr(C15H14O(CO3], displays a distorted envelope configuration of the dihydropyrane ring. The dihedral angle between the phenyl and phenylene rings is 50.63 (4°. The Cr0 atom is coordinated by three CO groups and the phenylene ring of the flavan ligand in an η6 mode, with a common arene-to-metal distance

  15. Preparing photochromic nanofibers and animal cells using a photochromic compound of 1′,3′,3′-trimethyl-6-nitrospiro (2H-1-benzopyran-2,2′-indoline)

    DEFF Research Database (Denmark)

    Li, Xiaoqiang; Lin, Lin; Kanjwal, Muzafar Ahmed

    2012-01-01

    mixed solutions of NOSP and polymers (including a synthetic polymer of poly(methyl methacrylate) and a natural polymer of gelatin); NOSP/ethanol solution was dissolved in culture medium to stain pig iliac endothelial cells (PIEC) and endow them with photochromic capability. Polymer nanofibers from...... electrospinning were characterized by water contact angle measurements, ultraviolet–visible (UV–Vis) spectrophotometry and fluorescence microscopy. Morphology of photochromic PIEC was observed by fluorescence microscopy after being irradiated. It was shown that nanofibers from electrospun polymers and NOSP...

  16. Phenolics from Brazilian propolis

    OpenAIRE

    1997-01-01

    The main phenolic constituents from Brazilian propolis, originating from Sao Paulo State, were isolated and identified: three flavonoids, a prenylated coumaric acid and two new benzopyranes, E and Z 2,2-dimethyl-6-carboxyethenyl-8-prenyl-2H-benzopyranes.

  17. Synthesis of novel photochromic pyrans via palladium-mediated reactions

    NARCIS (Netherlands)

    Böttcher, Christoph; Zeyat, Gehad; Ahmed, Saleh A.; Irran, Elisabeth; Cordes, Thorben; Elsner, Cord; Zinth, Wolfgang; Rueck-Braun, Karola

    2009-01-01

    Photochromic pyrans for applications in material and life sciences were synthesized via palladium-mediated cyanation, carbonylation and Sonogashira cross-coupling starting from a bromo-substituted naphthopyran and benzopyrans. A novel photoswitchable benzopyran-based ω-amino acid for Fmoc-based soli

  18. Synthesis of novel photochromic pyrans via palladium-mediated reactions

    Directory of Open Access Journals (Sweden)

    Christoph Böttcher

    2009-05-01

    Full Text Available Photochromic pyrans for applications in material and life sciences were synthesized via palladium-mediated cyanation, carbonylation and Sonogashira cross-coupling starting from bromo-substituted naphthopyran 1 and benzopyrans 2a/b. A novel photoswitchable benzopyran-based ω-amino acid 6 for Fmoc-based solid-phase peptide synthesis is presented. The photochromic behaviour of the 3-cyano-substituted benzopyran 5a was investigated by time-resolved absorption spectroscopy in the picosecond time domain.

  19. Facile solvent-free one-pot synthesis of pyranobenzopyrans and their derivatives

    Indian Academy of Sciences (India)

    P Sirisha; Suhas Pednekar; Nant R Kapdi; Minal Naik

    2011-09-01

    The condensation of hydroxyl coumarins in a facile one-pot procedure with active methylene esters to afford novel benzopyrans under solventless conditions is described. The products underwent further condensation and cyclization reactions to form novel heterocycles.

  20. Study on NO-Donating Antihypertensive Agents Ⅰ. Synthesis and Antihypertensive Activity of C-3 Nitrate or Furoxan Substituted Benzopyrans%一氧化氮供体型抗高血压药物的研究Ⅰ.C-3位具有硝酸酯和呋咱氮氧化物取代结构的苯骈吡喃类化合物的合成及其降压活性

    Institute of Scientific and Technical Information of China (English)

    许忻; 张奕华; 彭司勋; 季晖; 李永齐

    2005-01-01

    目的:研究C-3位一氧化氮(NO)供体取代的苯骈吡喃类化合物的合成及其降压活性,寻找活性强、不良反应小的新型抗高血压药.方法:以3,4位反式苯骈吡喃为基本骨架,在C-3位通过丁二酸连接硝酸酯和呋咱氮氧化物,合成了一系列衍生物;测定目标物对KCl引起的大鼠胸主动脉条收缩的抑制作用,选择活性化合物Ⅱ1测定其经口给药对自发性高血压大鼠(SHR)尾动脉收缩压(SAP)和舒张压(DAP)的影响;采用Griess法测定目标物体外NO释放量.结果与结论:合成了16个新化合物(Ⅰ1-6;Ⅱ1-10),结构经波谱确证.大部分目标物对大鼠胸主动脉条收缩具有不同程度的抑制作用,其中Ⅱ1的抑制率与阳性药吡那地尔(PIN)相当,对SHR尾动脉SAP和DAP的抑制幅度分别为15.2%和12.5%,降压持续时间长于PIN.Ⅱ1的NO释放量为0.9μg/mL,NO释放量与目标物降压活性的关系尚待进一步研究.

  1. Synthesis of novel furobenzopyrone derivatives and evaluation of their antimicrobial and antiinflammatory activity

    Directory of Open Access Journals (Sweden)

    Srinivasan K

    2007-01-01

    Full Text Available Certain 4′-(4′′-substituted phenyl-4-methylfurobenzopyrones were synthesized and evaluated for antibacterial activity. Six of the synthesized compounds were also screened for their antiinflammatory activity. Substituted resorcinols were condensed with ethyl acetoacetate to afford different coumarins (2a-c. Various substituted phenacyl bromides (4a-g were prepared by the bromination of para-substituted acetophenones. The coumarins (2a-c and phenacyl bromides (4a-g were condensed to give oxoethers (5a-s. These were cyclised by using 1 M sodium hydroxide to afford the desired furobenzopyrone derivatives (FCa-s. All the compounds have been evaluated for their antibacterial activity against different strains of gram positive and gram negative bacteria. All the compounds have shown good activity against Pseudomonas aeruginosa . Compounds, 3-(4-chlorophenyl-5-methylfuro-[3,2-g][1]benzopyran-7-one, 3-(4-chlorophenyl-5,9-dimethylfuro[3,2-g][1]benzopyran-7-one and 4,5-dimethyl-3-phenylfuro[3,2-g][1]benzopyran-7-one (FCe, FCi, FCn were active against E. coli . A few compounds showed moderate activity against Bacillus subtilis also. Antiinflammatory activity of six selected compounds was also tested using the carrageenan-induced rat paw oedema method. Among them, 5-methyl-3-p-tolylfuro[3,2-g][1]benzopyran-7-one (FCg showed excellent activity. 5-Methyl-3-phenylfuro[3,2-g][1]benzopyran-7-one (FCa and 4,5-dimethyl-3-(4-nitrophenyl-furo[3,2-g][1]benzopyran-7-one (FCc showed activity comparable to that of the standard drug ibuprofen.

  2. A Convenient Method to Construct Chroman Skeleton: Asymmetric Synthesis of (-)-4'-Hydroxy-7-methoxyflavane

    Institute of Scientific and Technical Information of China (English)

    XU, Boyan; XUE, Jijun; ZHANG, Huabing; LI, Ying

    2009-01-01

    The first enantioselective synthesis of a naturally occurring 4'-hydroxy-7-methoxyflavane was developed by an asymmetric reduction and a microwave-assistant aromatic C-O bond formation to construct the benzopyran skele- ton. Key features of this method include its brevity, its preserved stereochemical integrity and two different syn- thetic routes to choose.

  3. Multifunctional Spirocyclic Systems

    Directory of Open Access Journals (Sweden)

    Boris Lukyanov

    2009-01-01

    Full Text Available New spiropyrans with different substituents in the benzopyran fragment have been synthesized and investigated. It was shown that introduction of aldehyde group in the structure of spiropyrans gives a possibility to obtain new functionalized compounds. Effects of the substituents on the photochromic behavior were studied.

  4. Secondary metabolites characteristic of Penicillium citrinum, Penicillium steckii and related species

    DEFF Research Database (Denmark)

    Malmstrom, J.; Christophersen, C.; Frisvad, Jens Christian

    2000-01-01

    an unidentified tunicate. The carboxylic acids and the benzopyran were identified on the basis of mass spectrometry, and one and two dimensional NMR spectroscopic techniques. The structures 1 and 2 resemble tanzawaic acid A-D, previously isolated from Penicillium citrinum. Screening of isolates of species related...

  5. Synthesis and Biological Evaluation of Some New Coumarin Derivatives

    Directory of Open Access Journals (Sweden)

    M. Y. El-Kady

    2003-02-01

    Full Text Available Pyrimidino[5`,4`-6,5]-,pyridino[3`,2`-6,5]- and pyrrolo[3`,2`-5,6]4H-pyrano-[3,2-c][1]benzopyran-6-one derivatives (5-7 and 10 could be obtained via reaction of 2-amino-4-(p-bromophenyl-3-cyano(carboethoxy-4H,5H-pyrano[3,2-c][1]benzopyran-5-ones (3a,b with a variety of reagents. Alkylation of (3b with either 2-furoyl chloride or chloroacetyl chloride gave the 2-N-substituted derivatives (9a,b. Benzofurano[3,2-b]4H-pyran derivative (12 was also prepared. The antimicrobial activity of the prepared compounds was tested.

  6. Synthesis of Benzofuran Derivatives via Rearrangement and Their Inhibitory Activity on Acetylcholinesterase

    Directory of Open Access Journals (Sweden)

    Ling-Yi Kong

    2010-11-01

    Full Text Available During a synthesis of coumarins to obtain new candidates for treating Alzheimer’s Disease (AD, an unusual rearrangement of a benzopyran group to a benzofuran group occurred, offering a novel synthesis pathway of these benzofuran derivatives. The possible mechanism of the novel rearrangement was also discussed. All of the benzofuran derivatives have weak anti-AChE activities compared with the reference compound, donepezil.

  7. A new sesquiterpenoid coumarin from Ferula assafoetida.

    Science.gov (United States)

    Ghosh, Anindita; Banerji, Avijit; Mandal, Suvra; Banerji, Julie

    2009-08-01

    The gum resin of Ferula assafoetida Linn. afforded one new sesquiterpene, asimafoetida 1 and three known sesquiterpenoid coumarins. The structure has been established from extensive 2D NMR spectral studies as 7'-[7-(1R,3S)-5-Hydroxy-6,6-dimethyl-2-methylene-cyclohexyl]-9-methyl-9-pentenyl]oxy]-2H-1-benzopyran-2-one. The other three known compounds are ferulic acid, farnesiferol A and farnesiferol C.

  8. A New Pyrone Derivative from Fungus Cephalosporium sp. AL031

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    A novel pyrone derivative was isolated from the ethyl acetate extract of a culture broth of a strain of the fungus Cephalosporium sp. AL031. Its structure was characterized as 7-hydroxy-9-methoxyl-10-methyl-2H, 4aH, 6H, 10bH-pyrano[5, 6-c][2]benzopyran-2, 6-dione by spectroscopic analysis including IR, MS, 1D and 2D NMR spectra.

  9. A New Chromone Derivative from Stellera chamaejasme L.

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    A new chromone derivative, 3-[1- (2, 4, 6-trihydroxyphenyl) 3-di-(4-hydroxyphenyl) 1-propanone-2-yl] 5,7-dihydroxy-8-di(4-hydroxyphenyl)methyl-4H-1-benzopyran-4-one, named as isomohsenone was isolated from the roots of Stellera chamaejasme L. together with known chamaechromone. Its structure was determined by the analysis of MS and NMR data, especially 2D NMR spectra.

  10. Bioconcentration and acute toxicity of polycyclic musks in two benthic organisms (Chironomus riparius and Lumbriculus variegatus)

    NARCIS (Netherlands)

    Artola-Garicano, E.; Sinnige, T.L.; Holsteijn, I. van; Vaes, W.H.J.; Hermens, J.L.M.

    2003-01-01

    In the current study, the bioconcentration behavior and acute toxicity of two polycyclic musks, Tonalide® 7-acetyl-1,1,3,4,4,6,-hexamethyl-1,2,3,4,-tetrahydronaphthalene (AHTN) and Galaxolide® 1,3,4,6,7,8-hexahydro-4,6,6,7,8,8-hexa-methyl-cyclopenta[γ]-2- benzopyran (HHCB), were studied in two benth

  11. Meranzin hydrate from Muraya paniculata

    Directory of Open Access Journals (Sweden)

    Khalijah Awang

    2010-03-01

    Full Text Available The coumarin ring system in the title compound, C15H18O5 [IUPAC name: 8-(2,3-dihydroxy-3-methylbutyl-7-methoxy-2H-1-benzopyran-2-one], isolated from Muraya paniculata, is planar (r.m.s. deviation 0.017 Å. In the crystal, the two hydroxy groups are involved in O—H...O hydrogen bonding with adjacent molecules, forming a sheet structure.

  12. Influence of the Solvent on the Thermal Back Reaction of One Spiropyran

    Science.gov (United States)

    Piard, Jonathan

    2014-01-01

    The solvent influence on the absorption spectra and the kinetics of the back reaction of the 1',3'-dihydro-1',3',3'-trimethyl-6-nitrospiro(2H-1-benzopyran-2,2'-2H-indole) (6-NO2-BIPS) has been investigated by means of temperature-controlled, UV-visible spectroscopic measurements. The back reaction process was proved to follow first-order kinetics…

  13. Isolation and structure elucidation of a new prenylcoumarin from Murraya paniculata var. omphalocarpa (Rutaceae).

    Science.gov (United States)

    Kinoshita, Takeshi; Shimada, Motoko

    2002-01-01

    A new C-8 prenylated 5,7-dimethoxycoumarin named omphamurrayin was isolated from the leaves of Murraya paniculata var. omphalocarpa, and its structure was established as 5,7-dimethoxy-8-(1-oxo-2-senecioyl-3-methyl-3-butenyl)-2H-1-benzopyran-2-one on the basis of the spectroscopic evidence. The taxonomic status of M. paniculata var. omphalocarpa is briefly discussed, along with its synonymity to M. paniculata from the chemosystematic viewpoint.

  14. Three isoflavanones with cannabinoid-like moieties from Desmodium canum.

    Science.gov (United States)

    Botta, B; Gacs-Baitz, E; Vinciguerra, V; Delle Monache, G

    2003-09-01

    Three further derivatives of 5,7,2',4'-tetrahydroxy-6-methyl isoflavanone have been isolated from the root extract of Desmodium canum and assigned the structures 2,3-dihydro-5,7-dihydroxy-6-methyl-3-(1a,2,3,3a,8b,8c-hexahydro-6-hydroxy-1,1,3a-trimethyl-1H-4-oxabenzo[f]cyclobut[c,d]inden-7-yl)-4H-1-benzopyran-4-one (1) 2,3-dihydro-5,7-dihydroxy-6-methyl-3-(6a,7,8,10a-tetrahydro-3-hydroxy-6,6,9-trimethyl-6H-dibenzo[b,d]pyran-2-yl)-4H-1-benzopyran-4-one (2) 2,3-dihydro-5,7-dihydroxy-6-methyl-3-(3-hydroxy-6,6,9-trimethyl-6H-dibenzo[b,d]pyran-2-yl) 4H-1-benzopyran-4-one (3). The three compounds and the previously isolated chromene 4 all derive from the geranylated precursor 5 by a series of cannabinoid-like oxidative rearrangements.

  15. Phytochemical Analysis, Biological Activity, and Secretory Structures of Stachys annua (L.) L. subsp. annua (Lamiaceae) from Central Italy.

    Science.gov (United States)

    Venditti, Alessandro; Bianco, Armandodoriano; Quassinti, Luana; Bramucci, Massimo; Lupidi, Giulio; Damiano, Silvia; Papa, Fabrizio; Vittori, Sauro; Maleci Bini, Laura; Giuliani, Claudia; Lucarini, Domenico; Maggi, Filippo

    2015-08-01

    Stachys annua subsp. annua, well-known in central Italy as 'stregona annuale', is an annual, small, slightly-scented herb, commonly found in fields and uncultivated areas in almost all regions of Italy. In folk medicine, its aerial parts were used as anti-catarrhal, febrifuge, tonic, and vulnerary. In the present work, the chemical composition of the flowering aerial parts was studied. The hydrodistilled volatile oil, analysed by GC/MS, showed sesquiterpenoids as the major fraction (42.5%); phytol (9.8%), germacrene D (9.2%), and spathulenol (8.5%) were the most abundant constituents. The volatile oil was assayed for antioxidant and cytotoxic activity by DPPH, ABTS, FRAP, and MTT methods. The cytotoxicity results against HCT116, A375, and MDA-MB 231 human tumor cell lines were significant, with IC50 values of 23.5, 37.2, and 41.5 μg/ml, respectively, whereas the antioxidant power was negligible. The EtOH extract was composed mainly of three glycosidic flavonoids, namely 7-{[2-O-(6-O-acetyl-β-D-allopyranosyl)-β-D-glucopyranosyl]oxy}-5,8-dihydroxy-2-(4-methoxyphenyl)-4H-1-benzopyran-4-one (1), 7-{[6-O-acetyl-2-O-(6-O-acetyl-β-D-allopyranosyl)-β-D-glucopyranosyl]oxy}-2-(3,4-dihydroxyphenyl)-5,8-dihydroxy-4H-1-benzopyran-4-one (2), and 7-{[6-O-acetyl-2-O-(β-D-allopyranosyl)-β-D-glucopyranosyl]oxy}-2-(3-hydroxy-4-methoxyphenyl)-5,8-dihydroxy-4H-1-benzopyran-4-one (3). On the contrary, iridoids, considered chemotaxonomic markers of the genus Stachys, were absent in this species. Finally, the morphological and histochemical survey showed that glandular trichomes were composed of two main types, i.e. peltate type A and capitate types B and C giving positive response for both lipids and polyphenols.

  16. Determinação de cumarina em extrato fluido e tintura de guaco por espectrofotometria derivada de primeira ordem Determination of coumarin in fluid extract and tinture of "guaco" by first derivative spectrophotometry

    Directory of Open Access Journals (Sweden)

    Adriana de Carvalho Osório

    2004-12-01

    Full Text Available O objetivo do trabalho foi desenvolver um método de doseamento de cumarina (1,2-benzopirano em extrato fluido e tintura de guaco (Mikania glomerata Sprengel. O método desenvolvido foi por espectrofotometria derivada de primeira ordem, que se mostrou preciso, exato, reprodutível e de fácil execução.The objective of this work was to develop a method for coumarin(1,2-benzopyran dosage in fluid extract and tinture of "guaco" (Mikania glomerata Sprengel. First derivative spectrophotometry was developed and proved to be accurate, exact, reprodutive and of easy execution.

  17. Studies on the Interaction between Rutin and DNA in the Absence and Presence of β-Cyclodextrin by Electrochemical and Spectroscopic Methods

    Institute of Scientific and Technical Information of China (English)

    YANG, Gong-Jun(杨功俊); XU, Jing-Juan(徐静娟); CHEN, Hong-Yuan(陈洪渊); LENG, Zong-Zhou(冷宗周)

    2004-01-01

    The interactions between rutin or the inclusion complex of rutin-β-cyclodextrin and DNA were investigated by means of cyclic voltammetry, UV-vis absorption spectroscopy and fluorescence emission spectroscopy. The apparent binding constant of rutin with DNA is found to be 2.9×104 L/mol. The results showed that the benzopyranic-4-one plane of rutin mainly intercalated into DNA in the absence of β-cyclodextrin, while the catecholic portion of rutin was located in the double helix of DNA in the presence of β-cyclodextrin.

  18. Blocking effect of NIP-142 on the KCNQ1/KCNE1 channel current expressed in HEK293 cells.

    Science.gov (United States)

    Namekata, Iyuki; Tsuruoka, Noriko; Tsuneoka, Yayoi; Matsuda, Tomoyuki; Takahara, Akira; Tanaka, Yoshio; Suzuki, Takeshi; Takahashi, Tetsuo; Iida-Tanaka, Naoko; Tanaka, Hikaru

    2011-01-01

    We examined the effect of NIP-142, a benzopyran compound with terminating effect on experimental atrial arrhythmia, on the KCNQ1/KCNE1 channel, which underlies the slow component of the cardiac delayed rectifier potassium channel (I(Ks)). NIP-142, as well as chromanol 293B, showed concentration-dependent blockade of the current expressed in HEK293 cells; the EC(50) value of NIP-142 and chromanol 293B for the inhibition of tail current was 13.2 µM and 4.9 µM, respectively. These results indicate that NIP-142 has blocking effect on the KCNQ1/KCNE1 channel current.

  19. Effect of NIP-142 on potassium channel alpha-subunits Kv1.5, Kv4.2 and Kv4.3, and mouse atrial repolarization.

    Science.gov (United States)

    Tanaka, Hikaru; Namekata, Iyuki; Hamaguchi, Shogo; Kawamura, Taro; Masuda, Hiroyuki; Tanaka, Yoshio; Iida-Tanaka, Naoko; Takahara, Akira

    2010-01-01

    Effects of NIP-142, a benzopyran compound which terminates experimental atrial arrhythmia, on potassium channel alpha-subunits and mouse atrial repolarization were examined. NIP-142 concentration-dependently blocked the outward current through potassium channel alpha subunits Kv1.5, Kv4.2 and Kv4.3 expressed in Xenopus oocytes. In isolated mouse atrial myocardia, NIP-142 prolonged the action potential duration and effective refractory period, and increased the contractile force. These results suggest that NIP-142 blocks the potassium channels underlying the transient and sustained outward currents, which may contribute to its antiarrhythmic activity.

  20. Individual- and population-level effects of the synthetic musk, HHCB, on the deposit-feeding polychaete, Capitella sp. I

    DEFF Research Database (Denmark)

    Ramskov, Tina; Selck, Henriette; Salvito, Daniel;

    2009-01-01

    A life table response experiment lasting 120 d was used to investigate the effects of the synthetic polycyclic musk HHCB (1,3,4,6,7,8-hexahydro-4,6,6,7,8-hexamethylcyclopenta-gamma-2-benzopyrane; 0, 1.5, 26, 123, and 168 mg/kg dry wt sediment) on the life history of the infaunal polychaete...... Capitella species I. The HHCB exposure showed no detectable effects on adult survival, age at first reproduction, length of the reproductive period, number of broods, individual worm body volumes, or body size-specific egestion rates. In contrast, HHCB significantly affected juvenile survival (>= 123 mg...

  1. Density Functional Theory Study of the Trans-Trans-Cis (TTC→Trans-Trans-Trans (TTT Isomerization of a Photochromic Spiropyran Merocyanine

    Directory of Open Access Journals (Sweden)

    Gianfranco La Manna

    2008-06-01

    Full Text Available Density Functional Theory (DFT calculations have been performed on the TTC→TTT isomerization reaction of the open forms of the 1',3'-dihydro-8-bromo-6-nitro- 1',3',3'-trimethylspiro[2H-1-benzopyran-2,2'-(2Hindole (8-Br-6-nitro-BIPS system. The calculations were carried out in vacuo and in methylene chloride solution at different temperatures. Results are compared with the available experimental values of free energy difference and activation energy in solution.

  2. Synthesis of novel chalcone derivatives and their stabilization effect of spiropyran in PMMA films

    Institute of Scientific and Technical Information of China (English)

    Zheng Kai Si; Qing Zhang; Min Zhao Xue; Yuan Yuan Zhu; Liang Ming; Qiao Rong Sheng; Yan Gang Liu

    2011-01-01

    Three novel bis-chalcone derivatives with different alkyldioxy spacers were synthesized and dispersed into polymethyl methacrylate (PMMA) chloroform solution with 6-nitro-1'-ethyl-3',3'-dimethylspiro-2H-1-benzopyran-2,2'-indoline (ESP) to prepare photochromic PMMA films in a facile way. After irradiation with 365 nm UV light, the photocrosslinking reaction between chalcone units was proved to retard the decolorization of merocyanine form of the photochromic spiropyran effectively, as results of the steric hindrance produced by photocycloaddition of chalcone groups. It has been found that the bis-chalcone molecule with the shortest spacer has the most effective stabilizing effect on retardation of decoloration of spiropyran.

  3. New Polyketides and New Benzoic Acid Derivatives from the Marine Sponge-Associated Fungus Neosartorya quadricincta KUFA 0081

    Directory of Open Access Journals (Sweden)

    Chadaporn Prompanya

    2016-07-01

    Full Text Available Two new pentaketides, including a new benzofuran-1-one derivative (1 and a new isochromen-1-one (5, and seven new benzoic acid derivatives, including two new benzopyran derivatives (2a, b, a new benzoxepine derivative (3, two new chromen-4-one derivatives (4b, 7 and two new benzofuran derivatives (6a, b, were isolated, together with the previously reported 2,3-dihydro-6-hydroxy-2,2-dimethyl-4H-1-benzopyran-4-one (4a, from the culture of the marine sponge-associated fungus Neosartorya quadricincta KUFA 0081. The structures of the new compounds were established based on 1D and 2D NMR spectral analysis, and in the case of compounds 1, 2a, 4b, 5, 6a and 7, the absolute configurations of their stereogenic carbons were determined by an X-ray crystallographic analysis. None of the isolated compounds were active in the tests for antibacterial activity against Gram-positive and Gram-negative bacteria, as well as multidrug-resistant isolates from the environment (MIC > 256 μg/mL, antifungal activity against yeast (Candida albicans ATTC 10231, filamentous fungus (Aspergillus fumigatus ATTC 46645 and dermatophyte (Trichophyton rubrum FF5 (MIC > 512 µg/mL and in vitro growth inhibitory activity against the MCF-7 (breast adenocarcinoma, NCI-H460 (non-small cell lung cancer and A375-C5 (melanoma cell lines (GI50 > 150 µM by the protein binding dye SRB method.

  4. Blockade by NIP-142, an antiarrhythmic agent, of carbachol-induced atrial action potential shortening and GIRK1/4 channel.

    Science.gov (United States)

    Matsuda, Tomoyuki; Ito, Mie; Ishimaru, Sayoko; Tsuruoka, Noriko; Saito, Tomoaki; Iida-Tanaka, Naoko; Hashimoto, Norio; Yamashita, Toru; Tsuruzoe, Nobutomo; Tanaka, Hikaru; Shigenobu, Koki

    2006-08-01

    Mechanisms for the atria-specific action potential-prolonging action of NIP-142 ((3R*,4S*)-4-cyclopropylamino-3,4-dihydro-2,2-dimethyl-6-(4-methoxyphenylacetylamino)-7-nitro-2H-1-benzopyran-3-ol), a benzopyran compound that terminates experimental atrial arrhythmia, was examined. In isolated guinea-pig atrial tissue, NIP-142 reversed the shortening of action potential duration induced by either carbachol or adenosine. These effects were mimicked by tertiapin, but not by E-4031. NIP-142 concentration-dependently blocked the human G protein-coupled inwardly rectifying potassium channel current (GIRK1/4 channel current) expressed in HEK-293 cells with an EC50 value of 0.64 microM. At higher concentrations, NIP-142 blocked the human ether a go-go related gene (HERG) channel current with an EC50 value of 44 microM. In isolated guinea-pig papillary muscles, NIP-142 had no effect on the negative inotropic effect of carbachol under beta-adrenergic stimulation, indicating lack of effect on the muscarinic receptor and Gi protein. These results suggest that NIP-142 directly inhibits the acetylcholine-activated potassium current.

  5. Isolation of secondary metabolites from Hortia oreadica (Rutaceae) leaves through high-speed counter-current chromatography.

    Science.gov (United States)

    Severino, Vanessa Gisele Pasqualotto; Cazal, Cristiane de Melo; Forim, Moacir Rossi; da Silva, Maria Fátima das Graças Fernandes; Rodrigues-Filho, Edson; Fernandes, João Batista; Vieira, Paulo Cezar

    2009-05-08

    High-speed counter-current chromatography (HSCCC) with a two-phase solvent system (hexane-ethanol-acetonitrile-water 10:8:1:1, v/v) was applied to examine the leaves of Hortia oreadica, which afforded the known limonoid guyanin (1), the alkaloids rutaecarpin (2) and dictamnine (6), the dihydrocinnamic acid derivatives methyl 5,7-dimethoxy-2,2-dimethyl-2H-1-benzopyran-6-propanoate (3), 5,8-dimethoxy-2,2-dimethyl-2H-1-benzopyran-6-propanoic acid (4), together with the new E-3,4-dimethoxy-alpha(3-hydroxy-4-carbomethoxyphenyl)cinnamic acid (5). The recovery of compounds 1-6 was determined by comparison with LC-atmospheric pressure chemical ionization MS/MS data: 66.2%, 93.1%, 102.5%, 101.2%, 99.0% and 84.9%, respectively. Compound 3 showed IC(50) of 23.6microM against Plasmodium falciparum and 15.6microM against Trypanosoma brucei rhodesienses and was not toxic to KB cells (IC(50)>100microM).

  6. Chemical constituents from Sphallerocarpus gracilis%迷果芹根化学成分的研究

    Institute of Scientific and Technical Information of China (English)

    石明睿; 赵建喜; 冯祖飞; 柳军玺; 裴栋; 刘晔玮; 邸多隆

    2012-01-01

    Eleven compounds were isolated by silica gel column chromatography from Sphallerocarpus gracilis. Their structures were elucidated on the basis of spectral analysis and identified as falcarinol (1) ,(3.R,8S)-falcarindiol (2), diplaniol (3) ,5-hydroxy-8-methoxy-2H-1-benzopyran-2-one (4) ,esculetin (5) ,scopolin (6) ,xanthalin (7) ,isoimper-atorin (8) ,daucosterol (9) ,β-sitosterol (10) and stigmasterol (11). Compounds 1 ~6 were isolated form SphaUerocarpus gracilis for the first time.%利用普通硅胶柱色谱技术和多种现代波谱技术,从迷果芹(Sphallerocarpus gracilis)的根中分离并鉴定了11个化合物,通过波谱学方法鉴定为:falcarinol(1)、(3R,8S) -falcarindiol(2)、diplaniol(3)、5-hydroxy-8 -methoxy-2H-1-benzopyran-2-one(4)、esculetin(5)、东莨菪内酯(6)、xanthalin (7)、isoimperatorin(8)、胡萝卜苷(9)、β-谷甾醇(10)和豆甾醇(11),其中化合物1~6为首次从该植物中分离得到.

  7. New Polyketides and New Benzoic Acid Derivatives from the Marine Sponge-Associated Fungus Neosartorya quadricincta KUFA 0081

    Science.gov (United States)

    Prompanya, Chadaporn; Dethoup, Tida; Gales, Luís; Lee, Michael; Pereira, José A. C.; Silva, Artur M. S.; Pinto, Madalena M. M.; Kijjoa, Anake

    2016-01-01

    Two new pentaketides, including a new benzofuran-1-one derivative (1) and a new isochromen-1-one (5), and seven new benzoic acid derivatives, including two new benzopyran derivatives (2a, b), a new benzoxepine derivative (3), two new chromen-4-one derivatives (4b, 7) and two new benzofuran derivatives (6a, b), were isolated, together with the previously reported 2,3-dihydro-6-hydroxy-2,2-dimethyl-4H-1-benzopyran-4-one (4a), from the culture of the marine sponge-associated fungus Neosartorya quadricincta KUFA 0081. The structures of the new compounds were established based on 1D and 2D NMR spectral analysis, and in the case of compounds 1, 2a, 4b, 5, 6a and 7, the absolute configurations of their stereogenic carbons were determined by an X-ray crystallographic analysis. None of the isolated compounds were active in the tests for antibacterial activity against Gram-positive and Gram-negative bacteria, as well as multidrug-resistant isolates from the environment (MIC > 256 μg/mL), antifungal activity against yeast (Candida albicans ATTC 10231), filamentous fungus (Aspergillus fumigatus ATTC 46645) and dermatophyte (Trichophyton rubrum FF5) (MIC > 512 µg/mL) and in vitro growth inhibitory activity against the MCF-7 (breast adenocarcinoma), NCI-H460 (non-small cell lung cancer) and A375-C5 (melanoma) cell lines (GI50 > 150 µM) by the protein binding dye SRB method. PMID:27438842

  8. X-ray study of weak interactions in two flavonoids

    Indian Academy of Sciences (India)

    Deepak Sharma; Vivek K Gupta; Goutam Brahmachari; Sadhan Mondal; Arindam Gangopadhyay

    2007-10-01

    X-ray diffraction studies were carried out on single crystals of two flavonoids, viz. 5-hydroxy-6,7,4′-trimethoxyflavone, C18H16O6, (I) and 5-hydroxy-3,7,4′-trimethoxyflavone, C18H16O6, (II). Crystal structures of both the flavonoids were solved by direct methods and refined by full-matrix least-squares procedures. In both the molecules, the benzopyran moiety is planar. The dihedral angle between the phenyl ring and the benzopyran portion is 5.50(4)° in (I) and 29.11(5)° in (II). In (I), the crystal packing is influenced by O–H…O hydrogen bonds, and weak C–H…O and $\\pi \\ldots \\pi$ interactions whereas in (II) the crystal structure is stabilized by the presence of four intermolecular short contacts of the type C–H…O. There is also one C–H$\\ldots \\pi$ hydrogen bond with H… centroid distance of < 2.7 Å. The molecules are further stabilized by – interactions.

  9. The Effect of Tb and Sm Ions on the Photochromic Behavior of Two Spiropyrans of Benzoxazine Series in Solution

    Directory of Open Access Journals (Sweden)

    Esam Bakeir

    2008-01-01

    Full Text Available The photochromism of [7′-hydroxy-8′-formyl-3-methyl-4-oxospiro[1,3-benzoxazin-2,2′-[2H-1]benzopyran],SP(I,[7′-hydroxy-8′-formyl-3-benzyl-4-oxospiro[1,3-benzoxazin-2,2′-[2H-1]benzopyran] SP(II and their coordination with Tb3+and Sm3+ ions have been studied in DMF. UV/vis induced-color development due to heterolytic bond cleavage of SP(I and SP(II is greatly influenced by complexation with the lanthanide ions. The irradiation-induced color enhancement due to ring opening and thermal decoloration of the open forms of SP(I, SP(II follows first-order kinetics. Physical characteristics of the studied systems such as colorability and relaxation time of thermal bleaching parameters were determined. Moreover, light-energy transfer-induced luminescence of lanthanide ions via coordination with the two spirobenzoxazines was monitored.

  10. Synthesis and in vitro antioxidant activity evaluation of 3-carboxycoumarin derivatives and QSAR study of their DPPH• radical scavenging activity.

    Science.gov (United States)

    Martínez-Martínez, Francisco J; Razo-Hernández, Rodrigo Said; Peraza-Campos, Ana Lilia; Villanueva-García, Manuel; Sumaya-Martínez, Maria Teresa; Cano, Daniel Jaramillo; Gómez-Sandoval, Zeferino

    2012-12-13

    The in vitro antioxidant activities of eight 3-carboxycoumarin derivatives were assayed by the quantitative 1,1-diphenyl-2-picrylhydrazil (DPPH•) radical scavenging activity method. 3-Acetyl-6-hydroxy-2H-1-benzopyran-2-one (C1) and ethyl 6-hydroxy-2-oxo-2H-1-benzopyran-3-carboxylate (C2) presented the best radical-scavenging activity. A quantitative structure-activity relationship (QSAR) study was performed and correlated with the experimental DPPH• scavenging data. We used structural, geometrical, topological and quantum-chemical descriptors selected with Genetic Algorithms in order to determine which of these parameters are responsible of the observed DPPH• radical scavenging activity. We constructed a back propagation neural network with the hydrophilic factor (Hy) descriptor to generate an adequate architecture of neurons for the system description. The mathematical model showed a multiple determination coefficient of 0.9196 and a root mean squared error of 0.0851. Our results shows that the presence of hydroxyl groups on the ring structure of 3-carboxy-coumarins are correlated with the observed DPPH• radical scavenging activity effects.

  11. Pyridine analogues of spirocyclic σ₁ receptor ligands.

    Science.gov (United States)

    Miyata, Kengo; Möller, Guido; Schepmann, Dirk; Wünsch, Bernhard

    2014-08-01

    Spirocyclic benzopyrans 2 interact with high affinity and selectivity with σ₁ receptors. Bioisosteric replacement of the benzene ring of the benzopyran substructure with the electron rich thiophene ring (3) led to increased σ₁ affinity. Herein the synthesis and pharmacological evaluation of electron deficient pyridine bioisosteres 4 are reported. Homologation of the aldehyde 6 to afford the pyridylacetaldehyde derivative 8 was performed by a Wittig reaction. Bromine lithium exchange of the bromopyridine 8, addition to 1-benzylpiperidin-4-one and cyclization led to the spirocyclic pyrranopyridine 10. Hydrogenolytic removal of the N-benzyl moiety of 10 provided the secondary amine 11, which allowed the introduction of various N-substituents (12a-d). Cyclization of the hydroxy acetal 9 with HCl led to various modifications of the substituent in 3'-position. Generally the σ₁ affinity of the pyridine derivatives is reduced compared with those of the benzene and thiophene derivatives 2 and 3. However, the relationships between the structure and the σ₁ affinity follow the same rules as for the benzene and thiophene derivatives. The most promising σ₁ ligand within this class of compounds is the pyranopyridine 15 with a double bond in the pyran ring revealing a Ki-value of 4.6 nM and a very high selectivity (>217-fold) over the σ₂ subtype.

  12. 8,8-Dimethyl-8,9-dihydro-7H-chromeno[2,3-b]quinoline-10,12-dione

    Directory of Open Access Journals (Sweden)

    Thothadri Srinivasan

    2013-02-01

    Full Text Available In the title compound, C18H15NO3, the fused benzopyran and pyridine rings are essentially coplanar [r.m.s. deviation = 0.0533 Å with a maximum deviation of 0.080 (1 Å for a benzene C atom]. The cyclohexanone ring adopts an envelope conformation with the dimethyl-substituted C atom 0.660 (2 Å out of the plane formed by the remaining ring atoms (r.m.s. deviation = 0.0305 Å. The dihedral angle between the mean planes of the pyran and cyclohexanone rings is 12.95 (6°. In the crystal, molecules are linked via C—H...O hydrogen bonds, leading to chains running along [011].

  13. 腺梗豨莶的新双色满%A NEW BISCHROMAN FROM SIEGESBECKIA P UBESCENS

    Institute of Scientific and Technical Information of China (English)

    熊江; 许云龙

    2002-01-01

    作者从腺梗豨莶的乙醚抽出物中分到一个新的双色满成分,基于光谱分析和应用二维核磁共振技术,它的化学结构已被确定为3,3'-双(3,4-二氢化-6-甲氧基-2H-1-苯并吡喃)或3,3'-双(6-甲氧基色满).%A new bischroman (1) has been isolated from the diethyl ether extract of Siegesbeckia pubescens.Based on spectral analysis including 2D-NMR techniques, the structure of 1 was elucidated as 3,3' -bis(3,4-di-hydro-6-methoxy-2H-1-benzopyran) or 3,3' -bis(6-methoxychroman).

  14. (S,S,S,S)-Nebivolol hydro-chloride hemihydrate.

    Science.gov (United States)

    Rousselin, Yoann; Bruel, Amelie; Clavel, Alexandre

    2012-12-01

    The asymmetric unit of the title hydrated salt, C22H26F2NO4(+)·Cl(-)·0.5H2O, consists of an (S,S,S,S)-nebivolol {nebivol = bis-[2-(6-fluoro-3,4-dihydro-2H-1-benzopyran-2-yl)-2-hy-droxy-eth-yl]ammonium} cation, a chloride anion and a half-occupancy water mol-ecule. The dihedral angle between the mean planes of the benzene rings is 50.34 (12)°. The pyran rings adopt half-chair conformations. The crystal packing features O-H⋯O hydrogen bonds and weak N-H⋯Cl, O-H⋯Cl, and O-H⋯Cl inter-actions, producing layers along (010).

  15. (S,S,S,S-Nebivolol hydrochloride hemihydrate

    Directory of Open Access Journals (Sweden)

    Yoann Rousselin

    2012-12-01

    Full Text Available The asymmetric unit of the title hydrated salt, C22H26F2NO4+·Cl−·0.5H2O, consists of an (S,S,S,S-nebivolol {nebivol = bis[2-(6-fluoro-3,4-dihydro-2H-1-benzopyran-2-yl-2-hydroxyethyl]ammonium} cation, a chloride anion and a half-occupancy water molecule. The dihedral angle between the mean planes of the benzene rings is 50.34 (12°. The pyran rings adopt half-chair conformations. The crystal packing features O—H...O hydrogen bonds and weak N—H...Cl, O—H...Cl, and O—H...Cl interactions, producing layers along (010.

  16. Structure elucidation of a flavonoid glycoside from the roots of Clerodendrum serratum (L. Moon, Lamiaceae

    Directory of Open Access Journals (Sweden)

    S. S. Bhujbal

    2010-12-01

    Full Text Available Apigenin-7-glucoside, C21H20O10 (7-(β-D-glucopyranosyloxy-5-hydroxy-2-(4-hydroxyphenyl-4H-1-benzopyran-4-one, was first time isolated from the roots of Clerodendrum serratum (L. Moon, Lamiaceae. Structure elucidation of the compound was carried out by ¹H NMR and FAB-MS studies.Apigenin-7-glucosídeo, C21H20O10 (7-(β-D-glucopiranosiloxi-5-hidroxi-2-(4-hidroxifenil-4H-1-benzopiran-4-ona, foi isolado pela primeira vez das raízes de Clerodendrum serratum (L. Moon, Lamiaceae. A elucidação estrutural da susbtância foi feita através de estudos de ¹H NMR e FAB-MS.

  17. Pyranoxanthones: Synthesis, growth inhibitory activity on human tumor cell lines and determination of their lipophilicity in two membrane models

    DEFF Research Database (Denmark)

    Goncalves de Azavedo, Carlos M. B. P.; Afonso, C. M.; Soares, J. X.;

    2013-01-01

    The benzopyran and dihydrobenzopyran moieties can be considered as "privileged motifs" in drug discovery being good platforms for the search of new bioactive compounds. These moieties are commonly found fused to the xanthonic scaffold belonging to the biologically important family of the generally...... hard to be established. Accordingly, with the aim of rationalizing the importance of the fused ring orientation and oxygenation pattern in pyranoxanthones, this study describes the synthesis of 14 new pyranoxanthones and evaluation of their cell growth inhibitory activity in four human tumor cell lines...... as well as their lipophilicity in two membrane models. This systematic approach allowed establishing structure-activity and structure-lipophilicity relationships for the obtained compounds in combination with 6 previously described compounds. From this work an angular pyranoxanthone scaffold emerged...

  18. Structural Analysis of Spiropyran Polimers using ATR Spectroscopy

    Energy Technology Data Exchange (ETDEWEB)

    Delgado Macui, R; Rojas Lopez, M; Gayou, V L; Orduna Diaz, A [Centro de Investigacion en BiotecnologIa Aplicada del IPN, San Juan de los Molinos km. 1.5 Tepetitla de Lardizabal, A.P. 90700, Tlaxcala (Mexico)

    2006-01-01

    We have used infrared spectroscopy in attenuated total reflection (ATR) mode to analyze the interactions between the polymeric base and solvent with a photochromic material (spyropiran). We used cellulose acetate as polymeric base and the spyropiran; 1,3,3 trimethyl indoline-5-nitro benzopyrane. Thin films with different weight concentrations of SP were deposited in the polymeric base. The infrared spectra show bands whose frequencies are associated to several molecular bondings. It was observed a decreasing in intensity of absorbance for C = O stretching mode of the acetate group at 1720 cm{sup -1} and for C = C stretching mode for the main chain at 823 and 982 cm{sup -1} both associated to the presence of SP in polymeric films.

  19. A double-blind study of SB-220453 (Tonerbasat) in the glyceryltrinitrate (GTN) model of migraine

    DEFF Research Database (Denmark)

    Tvedskov, Jesper Filtenborg; Iversen, H K; Olesen, J

    2004-01-01

    The need for experimental migraine models increases as therapeutic options widen. In the present study, we investigated SB-220453 for efficacy in the glyceryltrinitrate (GTN) human experimental migraine model. SB-220453 is a novel benzopyran compound, which in animal models inhibits neurogenic...... inflammation, blocks propagation of spreading depression and inhibits trigeminal nerve ganglion stimulation-induced carotid vasodilatation. We included 15 patients with migraine without aura in a randomized double-blind crossover study. SB-220453 40 mg or placebo was followed by a 20-min GTN infusion. Headache...... no vascular or sympaticolytic activity with SB-220453. The study was terminated prematurely due to this interaction. GTN was consistent in producing headache and migraine that resembled the patients' usual spontaneous migraine. Nine patients had GTN on both study days. Peak headache score showed a trend...

  20. A double-blind study of SB-220453 (Tonerbasat) in the glyceryltrinitrate (GTN) model of migraine

    DEFF Research Database (Denmark)

    Tvedskov, Jesper Filtenborg; Iversen, H K; Olesen, J

    2004-01-01

    The need for experimental migraine models increases as therapeutic options widen. In the present study, we investigated SB-220453 for efficacy in the glyceryltrinitrate (GTN) human experimental migraine model. SB-220453 is a novel benzopyran compound, which in animal models inhibits neurogenic...... inflammation, blocks propagation of spreading depression and inhibits trigeminal nerve ganglion stimulation-induced carotid vasodilatation. We included 15 patients with migraine without aura in a randomized double-blind crossover study. SB-220453 40 mg or placebo was followed by a 20-min GTN infusion. Headache......, scored 0-10, was registered for 12 h, and fulfillment of International Headache Society (IHS) criteria was recorded until 24 h. Four subjects had a hypotensive episode after SB-220453 plus GTN but none after GTN alone. The reaction was unexpected, since animal models and previous human studies had shown...

  1. A homo-isoflavonoid and a cytotoxic saponin from Dracaena draco.

    Science.gov (United States)

    Hernández, Juan C; León, Francisco; Estévez, Francisco; Quintana, José; Bermejo, Jaime

    2006-01-01

    Two new compounds, dracol (= (3R)-2,3-dihydro-3,5-dihydroxy-7-methoxy-3-[(4-methoxyphenyl)methyl]-8-methyl-4H-[1]benzopyran-4-one; 1) and icodeside (= (1beta,3beta,23S,24S)-3,23-dihydroxy-1-{[2-O-(2,3,4-tri-O-acetyl-alpha-L-rhamnopyranosyl)-alpha-L-arabinopyranosyl]oxy}spirosta-5,25(27)-dien-24-yl alpha-L-arabinopyranoside; 2), were isolated from the EtOH extract of the leaves of Dracaena draco, together with 17 known constituents. The structures of 1 and 2 were elucidated by in-depth spectroscopic analysis, and those of the known compounds were identified by comparison of their NMR and MS data with those reported in the literature. Icodeside (2) showed moderate cytotoxicity against human HL-60 and A-431 cells (Table 3).

  2. Actividad antibacteriana de algunas especies medicinales de Eupatorium contra bacterias patógenas resistentes a antibióticos

    Directory of Open Access Journals (Sweden)

    Edgar García-Sánchez

    2015-07-01

    Full Text Available Thirty six samples arising from hexane, dichloromethane, and methanol extracts from four medicinal Eupatorium species were screened against Gram positive and Gram negative resistant bacteria associated with nosocomial infections by disk diffusion method. The best antibacterial extracts were selected by correspondence analysis. The hexane extracts showed high inhibition against clinical isolated bacteria. The qualitative chemical analysis was realized in the best antibacterial hexane extracts. The main components identified were triterpenes and benzopyran compounds which of extensively had been reported their antibacterial activities. Our results showing that medicinal Eupatorium species studied in this work have metabolites secondary antibacterial particularly against antibiotic resistant strains, Klebsiella pneumoniae and Staphylococcus aureus and validate their uses in traditional medicine

  3. Antifungal derivatives from Piper mollicomum and P. lhotzkyanum (Piperaceae)

    Energy Technology Data Exchange (ETDEWEB)

    Lago, Joao Henrique G. [Universidade Presbiteriana Mackenzie, Sao Paulo, SP (Brazil). Centro de Ciencias e Humanidades]. E-mail: joaolago@iq.usp.br; Young, Maria Claudia M. [Instituto de Botanica, Sao Paulo, SP (Brazil). Secao de Fisiologia e Bioquimica de Plantas; Reigada, Juliana B.; Soares, Marisi G.; Roesler, Bianca P.; Kato, Massuo J. [Universidade de Sao Paulo (USP), SP (Brazil). Inst. de Quimica

    2007-09-15

    Bioguided fractionation of the extracts from leaves of Piper mollicomum and Piper lhotzkyanum against the fungi Cladosporium cladosporioides and C. sphaerospermum afforded seven bioactive compounds, four being chromenes: methyl 2,2-dimethyl-2H-chromene-6-carboxylate, methyl 8-hydroxy-2,2-dimethyl-2H-chromene-6-carboxylate, 2-methyl-2-[4'-methyl-3'-pentenyl]-2H-1-benzopyrane-6-carboxylic acid, 2,2-dimethyl-2H-chromene-6-carboxylic acid, one a dihydrochalcone: 2',6'-dihydroxy-4'-methoxydihydrochalcone, and two flavanones: 7-methoxy-5,4'-dihydroxy-flavanone and 7,4'-dimethoxy-5-hydroxy-flavanone. The structures of the bioactive isolated derivatives were elucidated by interpretation of their NMR data [{sup 1}H and {sup 13}C (BBD, DEPT 135 deg)], and mass spectral data as well as by comparison with data described in the literature. (author)

  4. Protein structure similarity clustering and natural product structure as guiding principles for chemical genomics.

    Science.gov (United States)

    Koch, M A; Waldmann, H

    2006-01-01

    The majority of all proteins are modularly built from a limited set of approximately 1,000 structural domains. The knowledge of a common protein fold topology in the ligand-sensing cores of protein domains can be exploited for the design of small-molecule libraries in the development of inhibitors and ligands. Thus, a novel strategy of clustering protein domain cores based exclusively on structure similarity considerations (protein structure similarity clustering, PSSC) has been successfully applied to the development of small-molecule inhibitors of acetylcholinesterase and the 11beta-hydroxysteroid dehydrogenases based on the structure of a naturally occurring Cdc25 inhibitor. The efficiency of making use of the scaffolds of natural products as biologically prevalidated starting points for the design of compound libraries is further highlighted by the development of benzopyran-based FXR ligands.

  5. Prenylated flavone from roots of a hybrid between Artocarpus heterophyllus and Artocarpus integer and its biological activities

    Energy Technology Data Exchange (ETDEWEB)

    Panthong, Kanda, E-mail: kanda.p@psu.ac.th [Prince of Songkla University (Thailand). Natural Product Research Center of Excellence; Tohdee, Kanogwan [Prince of Songkla University (Thailand). Faculty of Science. Department of Chemistry; Hutadilok-Towatana, Nongporn [Prince of Songkla University (Thailand). Dept. of Biochemistry; Voravuthikunchai, Supayang P. [Prince of Songkla University (Thailand). Faculty of Science. Department of Microbiology; Chusri, Sasitorn [Prince of Songkla University (Thailand). Faculty of Traditional Thai Medicine

    2013-10-15

    One new prenylated flavone, 2,8-dihydroxy-3,10-dimethoxy-6-(2-methyl-1-propen-1-yl)- 6H,7H-[1]benzopyrano[4,3-b][1]-benzopyran-7-one, together with 24 known compounds were isolated from crude acetone extract from the roots of a hybrid between Artocarpus heterophyllus and Artocarpus integer. Their structures were determined by 1D and 2D nuclear magnetic resonance (NMR) spectroscopic data. The antioxidant and antibacterial activities of the isolated compounds were evaluated. The new compound showed potent antioxidant activity against DPPH Bullet and superoxide with IC{sub 50} values of 0.033 and 0.125 mg mL{sup -1}, respectively. Significant antibacterial activity against Acinetobacter baumannii was observed with MIC value of 50 {mu}g mL{sup -1}. (author)

  6. Spectral and photochromic study of spiropyran

    Energy Technology Data Exchange (ETDEWEB)

    Nordin, Rumaisa; Lazim, Azwani Mat; Hasbullah, Siti Aishah [School of Chemical Sciences and Food Technology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor Darul Ehsan (Malaysia)

    2013-11-27

    A new class of benzospiropyran has been synthesized by the condensation of the precursor, Fisher basespiropyran with substituted salicyaldehydes in 1:1 mole ratios. This photochromic compound, spiro [2H-1-benzopyran-2, 2’-(8’-hydroxy-1’, 3’, 3’-trimethylindoline)] was able to undergo convertion into its merocyanine form (colored) after being induced by irradiation of UV light. Both open and closed form of benzospiropyran were characterized and distinguished based on Infrared vibracional spectroscopy in Attenuated Total Reflexion mode (ATR) and UV-vis spectroscopy. In addition, association of ten different metal ions to ethanol solution of SP (A) leads to metal-ion binding by the MC isomer. The coupled reaction of all metal ions, MC-M{sup 2+} underwent a hypsochromic and bathochromic shift in the absorption bands.

  7. Five new secondary metabolites from Monascus purpureus-fermented Hordeum vulgare and Sorghum bicolor.

    Science.gov (United States)

    Ansari, Md Pravej; Puri, Alka; Ali, M; Panda, Bibhu Prasad

    2013-10-01

    Long grains of Hordeum vulgare and Sorghum bicolor were individually fermented with Monascus purpureus MTCC 369 under solid state fermentation. The aqueous extract of Monascus which fermented H. vulgare and S. bicolor was found to contain five different new metabolites. Silica gel column chromatography of the aqueous extract with a linear gradient of ethyl acetate, acetonitrile and carbon tetrachloride (v/v) yielded five new metabolites named benzopranyl capriate (9H-1-isoprenyl-benzopyran-5-isopropanoic acid-6-ol-6-n-decanoate), shorghumoic acid (n-octadec-8,11-dien-7α-ol-1-oic acid) and sorghumflavin A (2-n-butyloxo-6-β-hydroxy-7-β-isoprenyl ankaflavin) from Monascus-fermented S. bicolor, while hordeumflavin B (2-n-undecanyloxo-7-β-isoprenyl ankaflavin) and vulgaredilone (2-dodecanyl-7-β isopranyl monoscodilone) from Monascus-fermented H. vulgare.

  8. Kinetic model of metabolic network for xiamenmycin biosynthetic optimisation.

    Science.gov (United States)

    Xu, Min-juan; Chen, Yong-cong; Xu, Jun; Ao, Ping; Zhu, Xiao-mei

    2016-02-01

    Xiamenmycins, a series of prenylated benzopyran compounds with anti-fibrotic bioactivities, were isolated from a mangrove-derived Streptomyces xiamenensis. To fulfil the requirements of pharmaceutical investigations, a high production of xiamenmycin is needed. In this study, the authors present a kinetic metabolic model to evaluate fluxes in an engineered Streptomyces lividans with xiamenmycin-oriented genetic modification based on generic enzymatic rate equations and stability constraints. Lyapunov function was used for a viability optimisation. From their kinetic model, the flux distributions for the engineered S. lividans fed on glucose and glycerol as carbon sources were calculated. They found that if the bacterium can utilise glucose simultaneously with glycerol, xiamenmycin production can be enhanced by 40% theoretically, while maintaining the same growth rate. Glycerol may increase the flux for phosphoenolpyruvate synthesis without interfering citric acid cycle. They therefore believe this study demonstrates a possible new direction for bioengineering of S. lividans.

  9. Radiosynthesis and autoradiographic evaluation of [{sup 11}C]NAD-299, a radioligand for visualization of the 5-HT{sub 1A} receptor

    Energy Technology Data Exchange (ETDEWEB)

    Sandell, Johan E-mail: Johan.Sandell@psyk.ks.se; Halldin, Christer; Hall, Haakan; Thorberg, Seth-Olov; Werner, Tom; Sohn, Daniel; Sedvall, Goeran; Farde, Lars

    1999-02-01

    The selective 5-HT{sub 1A} receptor antagonist NAD-299 ([R]-3-N,N-dicyclobutylamino-8-fluoro-3,4-dihydro-2H-1-benzopyran- 5-carboxamide) was labeled with the positron emitting radionucldie carbon-11. The radioligand was synthesized from NAD-195 ([R]-3-N,N-dicyclobutylamino-8-fluoro-5-trifluoromethylsulfonyloxy-3, 4-dihydro-2H-1-benzopyran) in two radiochemical steps. A palladium-catalyzed reaction of NAD-195 and [{sup 11}C]cyanide was followed by hydrolysis of the carbon-11-labeled nitrile intermediate with basic hydrogen peroxide. The total radiochemical yield, based on [{sup 11}C]CO{sub 2} and corrected for decay, was 20-40%. The specific radioactivity was 24 GBq/{mu}mol (900 Ci/mmol) at end of synthesis, with a radiochemical purity better than 99% and a total synthesis time of 40-45 min. Autoradiographic examination of [{sup 11}C]NAD-299 binding in human brain postmortem demonstrated high binding in hippocampus, raphe nuclei, and neocortex. The binding in the hippocampus was higher than in the neocortex. Within the hippocampus, the densest binding was observed in the CA1 region. [{sup 11}C]NAD-299 binding was inhibited by addition of the 5-HT{sub 1A} receptor ligands WAY-100635, pindolol, ({+-})-8-OH-DPAT, 5-HT, and buspirone, leaving a low background of nonspecific binding. The results indicate that [{sup 11}C]NAD-299 binds specifically to 5-HT{sub 1A} receptors in the human brain in vitro and is a potential radioligand for positron emission tomography (PET) examination of 5-HT{sub 1A} receptors in vivo.

  10. QSAR analysis on Spodoptera litura antifeedant activities for flavone derivatives

    Energy Technology Data Exchange (ETDEWEB)

    Duchowicz, Pablo R., E-mail: pabloducho@gmail.com [Instituto de Investigaciones Fisicoquimicas Teoricas y Aplicadas INIFTA (UNLP, CCT La Plata-CONICET), Diag. 113 y 64, Sucursal 4, C.C. 16, 1900 La Plata (Argentina); Goodarzi, Mohammad [Instituto de Investigaciones Fisicoquimicas Teoricas y Aplicadas INIFTA (UNLP, CCT La Plata-CONICET), Diag. 113 y 64, Sucursal 4, C.C. 16, 1900 La Plata (Argentina); Ocsachoque, Marco A. [Centro de Investigacion y Desarrollo en Ciencias Aplicadas ' Dr. J. J. Ronco' (CINDECA), Departamento de Quimica, Facultad de Ciencias Exactas, UNLP-CONICET. Calle 47 No 257, B1900AJK La Plata (Argentina); Romanelli, Gustavo P. [Centro de Investigacion y Desarrollo en Ciencias Aplicadas ' Dr. J. J. Ronco' (CINDECA), Departamento de Quimica, Facultad de Ciencias Exactas, UNLP-CONICET. Calle 47 No 257, B1900AJK La Plata (Argentina); Catedra de Quimica Organica, Facultad de Ciencias Agrarias y Forestales, UNLP. Calles 60 y 119, B1904AAN La Plata (Argentina); Ortiz, Erlinda del V. [Facultad de Tecnologia y Ciencias Aplicadas, Universidad Nacional de Catamarca, Av. Maximio Victoria 55, (4700), Catamarca (Argentina); Autino, Juan C.; Bennardi, Daniel O.; Ruiz, Diego M. [Catedra de Quimica Organica, Facultad de Ciencias Agrarias y Forestales, UNLP. Calles 60 y 119, B1904AAN La Plata (Argentina); Castro, Eduardo A. [Instituto de Investigaciones Fisicoquimicas Teoricas y Aplicadas INIFTA (UNLP, CCT La Plata-CONICET), Diag. 113 y 64, Sucursal 4, C.C. 16, 1900 La Plata (Argentina)

    2009-12-20

    We establish useful models that relate experimentally measured biological activities of compounds to their molecular structure. The pED{sub 50} feeding inhibition on Spodoptera litura species exhibited by aurones, chromones, 3-coumarones and flavones is analyzed in this work through the hypothesis encompassed in the Quantitative Structure-Activity Relationships (QSAR) Theory. This constitutes a first necessary computationally based step during the design of more bio-friendly repellents that could lead to insights for improving the insecticidal activities of the investigated compounds. After optimizing the molecular structure of each furane and pyrane benzoderivative with the semiempirical molecular orbitals method PM3, more than a thousand of constitutional, topological, geometrical and electronic descriptors are calculated and multiparametric linear regression models are established on the antifeedant potencies. The feature selection method employed in this study is the Replacement Method, which has proven to be successful in previous analyzes. We establish the QSAR both for the complete molecular set of compounds and also for each chemical class, so that acceptably describing the variation of the inhibitory activities from the knowledge of their structure and thus achieving useful predictive results. The main interest of developing trustful QSAR models is that these enable the prediction of compounds having no experimentally measured activities for any reason. Therefore, the structure-activity relationships are further employed for investigating the antifeedant activity on previously synthesized 2-,7-substituted benzopyranes, which do not pose any measured values on the biological expression. One of them, 2-({alpha}-naphtyl)-4H-1-benzopyran-4-one, results in a promising structure to be experimentally analyzed as it has predicted pED{sub 50} = 1.162.

  11. Properties of Flavonoids Isolated from the Bark of Eysenhardtia polystachya and Their Effect on Oxidative Stress in Streptozotocin-Induced Diabetes Mellitus in Mice

    Science.gov (United States)

    Garcia-Campoy, Abraham Heriberto; Muñiz-Ramirez, Alethia

    2016-01-01

    Six new flavonoids 2′,4′-dihydroxychalcone-6′-O-β-d-glucopyranoside (1), α,3,2′,4′-tetrahydroxy-4-methoxy-dihydrochalcone-3′-C-β-glucopyranosy-6′-O-β-d-glucopyranoside (2), 7-hydroxy-5,8′-dimethoxy-6′α-l-rhamnopyranosyl-8-(3-phenyl-trans-acryloyl)-1-benzopyran-2-one (3), 6′7-dihydroxy-5,8-dimethoxy-8(3-phenyl-trans-acryloyl)-1-benzopyran-2-one (4), 9-hydroxy-3,8-dimethoxy-4-prenylpterocarpan (5), and α,4,4′-trihydroxydihydrochalcone-2′-O-β-d-glucopyranoside (6) were isolated from bark of Eysenhardtia polystachya. Antidiabetic activity of compounds 1–5 in terms of their cellular antioxidant and free radical scavenging and also in streptozotocin- (STZ-) induced diabetic mice was evaluated on liver transaminases, lipid peroxidation, total bilirubin, total protein, superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (CSH-Px), and glutathione reductase (GSH). Results indicated that 1–5 scavenged 2,2-diphenyl-1-picrylhydrazyl (DPPH), hydroxyl (∙OH), nitric oxide radicals (NO∙), superoxide anion radical (O2∙−), radical cation (ABTS∙+), and hydrogen peroxide (H2O2) radical, and protection against H2O2 induced BSA damage was also observed. Furthermore, 1–5 showed ability to decrease the oxidative stress in H9c2 cell. Diabetic mice present high levels of lipid peroxide, total protein, SGPT, SGOT, ALP, and TB. However, treatment of STZ-induced diabetes in mice with 1–5 reduced levels of these enzymes leading to protector effect of liver. In addition, with treatment with 1–5, increases in radical scavenging enzymes of CSH-Px, SOD, GSH, and CAT have also been observed in diabetic mice. The antioxidant properties of compounds 1–5 are a promising strategy for ameliorating therapeutic effects by avoiding disorders in the normal redox reactions in healthy cells which consequently could alleviate complications of diabetes. PMID:27668038

  12. Properties of Flavonoids Isolated from the Bark of Eysenhardtia polystachya and Their Effect on Oxidative Stress in Streptozotocin-Induced Diabetes Mellitus in Mice

    Directory of Open Access Journals (Sweden)

    Rosa Martha Perez-Gutierrez

    2016-01-01

    Full Text Available Six new flavonoids 2′,4′-dihydroxychalcone-6′-O-β-d-glucopyranoside (1, α,3,2′,4′-tetrahydroxy-4-methoxy-dihydrochalcone-3′-C-β-glucopyranosy-6′-O-β-d-glucopyranoside (2, 7-hydroxy-5,8′-dimethoxy-6′α-l-rhamnopyranosyl-8-(3-phenyl-trans-acryloyl-1-benzopyran-2-one (3, 6′7-dihydroxy-5,8-dimethoxy-8(3-phenyl-trans-acryloyl-1-benzopyran-2-one (4, 9-hydroxy-3,8-dimethoxy-4-prenylpterocarpan (5, and α,4,4′-trihydroxydihydrochalcone-2′-O-β-d-glucopyranoside (6 were isolated from bark of Eysenhardtia polystachya. Antidiabetic activity of compounds 1–5 in terms of their cellular antioxidant and free radical scavenging and also in streptozotocin- (STZ- induced diabetic mice was evaluated on liver transaminases, lipid peroxidation, total bilirubin, total protein, superoxide dismutase (SOD, catalase (CAT, glutathione peroxidase (CSH-Px, and glutathione reductase (GSH. Results indicated that 1–5 scavenged 2,2-diphenyl-1-picrylhydrazyl (DPPH, hydroxyl (OH∙, nitric oxide radicals (NO∙, superoxide anion radical (O2∙-, radical cation (ABTS∙+, and hydrogen peroxide (H2O2 radical, and protection against H2O2 induced BSA damage was also observed. Furthermore, 1–5 showed ability to decrease the oxidative stress in H9c2 cell. Diabetic mice present high levels of lipid peroxide, total protein, SGPT, SGOT, ALP, and TB. However, treatment of STZ-induced diabetes in mice with 1–5 reduced levels of these enzymes leading to protector effect of liver. In addition, with treatment with 1–5, increases in radical scavenging enzymes of CSH-Px, SOD, GSH, and CAT have also been observed in diabetic mice. The antioxidant properties of compounds 1–5 are a promising strategy for ameliorating therapeutic effects by avoiding disorders in the normal redox reactions in healthy cells which consequently could alleviate complications of diabetes.

  13. 云南地方晾晒烟化学成分研究%Study on Chemical Ingredients of Air-cured Tobacco in Yunnan Province

    Institute of Scientific and Technical Information of China (English)

    余文怡; 邓亮; 李兰; 徐兴梦; 郭亚东; 母多; 施建莲; 赵明智; 陈钰沁

    2015-01-01

    为研究云南大理云龙天登晾晒烟的化学成分,将烟叶用95%乙醇提取、浓缩,对浓缩液用乙酸乙酯萃取,将乙酸乙酯萃取部分进行系统化学成分分离,采用正相硅胶柱色谱及 MCI,PLgel,Sephadex LH-20,C18反相半制备高效液相色谱等柱色谱方法进行分离,根据理化性质和波谱数据对其结构鉴定。结果表明,从乙酸乙酯部分得到7个化合物,分别为7-Hydroxy-6-methoxy-2H-1-benzopyran-2-oneScopoletin(1),(7S,8R)-di-hydrodehydrodiconiferyl alcohol(2),N-trans-feruloyltyramine(3),N-cis-feruloyltyramine(4),香草酸(5),对羟基苯乙醇(6),3,4-二羟基苯甲酸(7)。其中除化合物5外,其余6个化合物均为首次从该烟草中分离鉴定。%To study the chemical constituents of Air-cured tobacco selected from Yunlong Tianden Dali,Yunnan province,95% ethanol was used to extract the tobacco leaf;the ethyl acetate part was isolated and purified by repeated column chromatography on silica gel, MCI,PLgel,Sephadex LH-20,C1 8 HPLC.The structures of chemical constituents were identified according to the physicochemical prop-erty and spectral data of compounds.The result showed that seven compounds were isolated from the ethyl acetate part identified as 7-Hydroxy-6-methoxy-2H-1 -benzopyran-2-oneScopoletin(1 ),(7S,8R)-dihydrodehydrodiconiferyl alcohol(2),N-trans-feruloyltyramine (3),N-cis-feruloyltyramine (4),vanillic acid (5),tyrosol(6),protocatechuic acid(7).Among them except No.5,other six ones are the first to be isolated and identified from the tobacco.

  14. The phytoestrogen genistein modulates lysosomal metabolism and transcription factor EB (TFEB) activation.

    Science.gov (United States)

    Moskot, Marta; Montefusco, Sandro; Jakóbkiewicz-Banecka, Joanna; Mozolewski, Paweł; Węgrzyn, Alicja; Di Bernardo, Diego; Węgrzyn, Grzegorz; Medina, Diego L; Ballabio, Andrea; Gabig-Cimińska, Magdalena

    2014-06-13

    Genistein (5,7-dihydroxy-3-(4-hydroxyphenyl)-4H-1-benzopyran-4-one) has been previously proposed as a potential drug for use in substrate reduction therapy for mucopolysaccharidoses, a group of inherited metabolic diseases caused by mutations leading to inefficient degradation of glycosaminoglycans (GAGs) in lysosomes. It was demonstrated that this isoflavone can cross the blood-brain barrier, making it an especially desirable potential drug for the treatment of neurological symptoms present in most lysosomal storage diseases. So far, no comprehensive genomic analyses have been performed to elucidate the molecular mechanisms underlying the effect elicited by genistein. Therefore, the aim of this work was to identify the genistein-modulated gene network regulating GAG biosynthesis and degradation, taking into consideration the entire lysosomal metabolism. Our analyses identified over 60 genes with known roles in lysosomal biogenesis and/or function whose expression was enhanced by genistein. Moreover, 19 genes whose products are involved in both GAG synthesis and degradation pathways were found to be remarkably differentially regulated by genistein treatment. We found a regulatory network linking genistein-mediated control of transcription factor EB (TFEB) gene expression, TFEB nuclear translocation, and activation of TFEB-dependent lysosome biogenesis to lysosomal metabolism. Our data indicate that the molecular mechanism of genistein action involves not only impairment of GAG synthesis but more importantly lysosomal enhancement via TFEB. These findings contribute to explaining the beneficial effects of genistein in lysosomal storage diseases as well as envisage new therapeutic approaches to treat these devastating diseases.

  15. Molecular interaction studies of green tea catechins as multitarget drug candidates for the treatment of Parkinson's disease: computational and structural insights.

    Science.gov (United States)

    Azam, Faizul; Mohamed, Najah; Alhussen, Fatma

    2015-01-01

    Green tea catechins have extensively been studied for their imminent role in reducing the risk of various neurodegenerative diseases such as Parkinson's disease (PD). Understanding the molecular interaction of these compounds with various anti-Parkinsonian drug targets is of interest. The present study is intended to explore binding modes of catechins with molecular targets having potential role in PD. Lamarckian genetic algorithm methodology was adopted for molecular docking simulations employing AutoDock 4.2 program. Toxicity potential and molecular properties responsible for good pharmacokinetic profile were calculated by Osiris property explorer and Molinspiration online toolkit, respectively. A strong correlation coefficient (r(2) = 0.893) was obtained between experimentally reported and docking predicted activities of native co-crystallized ligands of the 18 target receptors used in current study. Analysis of docked conformations revealed monoamine oxidase-B as most promising, while N-methyl-D-aspartate receptor was recognized as the least favorable target for catechins. Benzopyran skeleton with a phenyl group substituted at the 2-position and a hydroxyl (or ester) function at the 3-position has been identified as common structural requirements at majority of the targets. The present findings suggest that epigallocatechin gallate is the most promising lead to be developed as multitarget drug for the design and development of novel anti-Parkinsonian agents.

  16. UV-responsive polyvinyl alcohol nanofibers prepared by electrospinning

    Energy Technology Data Exchange (ETDEWEB)

    Khatri, Zeeshan, E-mail: zeeshan.khatri@faculty.muet.edu.pk [Department of Textile Engineering, Mehran University of Engineering and Technology, Jamshoro 76062 (Pakistan); Nano Fusion Technology Research Lab, Division of Frontier Fibers, Institute for Fiber Engineering (IFES), Interdisciplinary Cluster for Cutting Edge Research (ICCER), Shinshu University, 3-15-1, Tokida, Ueda, Nagano 386-8567 (Japan); Ali, Shamshad [Department of Textile Engineering, Mehran University of Engineering and Technology, Jamshoro 76062 (Pakistan); Department of Organic and Nano Engineering, Hanyang University, 17 Haengdang-dong, Seongdong-gu, Seoul 133-791 (Korea, Republic of); Khatri, Imran [Department of Entomology, Sindh Agriculture University, Tandojam (Pakistan); Mayakrishnan, Gopiraman [Nano Fusion Technology Research Lab, Division of Frontier Fibers, Institute for Fiber Engineering (IFES), Interdisciplinary Cluster for Cutting Edge Research (ICCER), Shinshu University, 3-15-1, Tokida, Ueda, Nagano 386-8567 (Japan); Kim, Seong Hun [Department of Organic and Nano Engineering, Hanyang University, 17 Haengdang-dong, Seongdong-gu, Seoul 133-791 (Korea, Republic of); Kim, Ick-Soo, E-mail: kim@shinshu-u.ac.jp [Nano Fusion Technology Research Lab, Division of Frontier Fibers, Institute for Fiber Engineering (IFES), Interdisciplinary Cluster for Cutting Edge Research (ICCER), Shinshu University, 3-15-1, Tokida, Ueda, Nagano 386-8567 (Japan)

    2015-07-01

    Graphical abstract: - Highlights: • UV responsive PVA nanofibers were prepared via electrospinning. • Quick response codes were recorded multiple times on UV responsive nanofibers. • The rate of photo-coloration was found faster than the rate of photo-reversibility. - Abstract: We report UV-responsive polyvinyl alcohol (PVA) nanofibers for potential application for recording and erasing quick response (QR) codes. We incorporate 1′-3′-dihydro-8-methoxy-1′,3′,3′-trimethyl-6-nitrospiro [2H-1-benzopyran-2,2′-(2H)-indole] (indole) and,3-dihydro-1,3,3-trimethylspiro [2H-indole-2,3′-[3H] phenanthr [9,10-b] (1,4) oxazine] (oxazine) into PVA polymer matrix via electrospinning technique. The resultant nanofibers were measured for recording–erasing, photo-coloration and thermal reversibility. The rate of photo-coloration of PVA–indole nanofibers was five times higher than the PVA–oxazine nanofibers, whereas the thermal reversibility found to be more than twice as fast as PVA–oxazine nanofibers. Results showed that the resultant nanofibers have very good capability of recording QR codes multiple times. The FTIR spectroscopy and SEM were employed to characterize the electrospun nanofibers. The UV-responsive PVA nanofibers have great potentials as a light-driven nanomaterials incorporated within sensors, sensitive displays and in optical devices such as erasable and rewritable optical storage.

  17. Effect of pH on the complexation of kaempferol-4'-glucoside with three β-cyclodextrin derivatives: isothermal titration calorimetry and spectroscopy study.

    Science.gov (United States)

    Zheng, Yan; Dong, Li-Na; Liu, Min; Chen, Aiju; Feng, Shangcai; Wang, Bingquan; Sun, Dezhi

    2014-01-08

    The utilization of kaempferol and its glycosides in food and pharmaceutical industries could be improved by the formation of inclusion complexes with cyclodextrins at different pH. This study explores the complexation of kaempferol-4'-glucoside with sulfobutyl ether-β-cyclodextrin (SBE-β-CD), hydroxypropyl-β-cyclodextrin (HP-β-CD), and methylated-β-cyclodextrin (M-β-CD) in phosphate buffer solutions of different pH using isothermal titration calorimetry, UV-vis absorption and proton nuclear magnetic resonance spectroscopy at 298.2 K. Experimental results showed that kaempferol-4'-glucoside binds with the three β- cyclodextrins in the same 1:1 stoichiometry. The rank order of stability constants is SBE-β-CD > HP-β-CD > M-β-CD at the same pH level and pH 6.0 > pH 7.4 > pH 9.0 for the same cyclodextrin. The binding of kaempferol-4'-glucoside with the three β-cyclodextrin derivatives is synergistically driven by enthalpy and entropy at pH 6.0 and enthalpy-driven at pH 7.4 and 9.0. The possible inclusion mode was that in the cavity of β-CD is included the planar benzopyranic-4-one part of the kaempferol-4'-glucoside.

  18. Supramolecular structure of S-(+)-marmesin-a linear dihydrofuranocoumarin

    Indian Academy of Sciences (India)

    Sanjeev Goswami; Vivek K Gupta; Ashok Sharma; B D Gupta

    2005-12-01

    The title compound, C14H14O4, a linear dihydrofuranocoumarin, was isolated from the bark of Aegle marmelos, a plant widely used in Ayurvedic system of medicine for the treatment of various ailments. The crystal structure was determined from X-ray diffraction data using direct methods. The compound crystallizes into monoclinic space group 21 with unit cell parameters: = 5.721(1) Å, = 13.810(1) Å, = 7.864(2) Å, = 100.39(1)°, = 2. The structure was refined by full-matrix least-squares to a final value of 0.0523 for 1184 observed reflections. The benzopyran moiety is perfectly planar. The dihedral angle between the pyrone and benzene rings is 0.3(1)°. The furan ring has a 2-envelope conformation. The molecules are linked by O–H…O hydrogen bonds into chains and these chains are linked into sheets by C–H…O hydrogen bonds. Further, the − stacking and C–H$\\ldots\\pi$(arene) interactions link all of the sheets into a supramolecular structure.

  19. Synthesis of 1-[6-Fluoro-(2S)-3H,4H-dihydro-2H-2-chromenyl]-(1R)-1,2- ethanediol and 1-[6-Fluoro-(2R)-3H,4H-dihydro-2H-2-chromenyl]- (1R)-1,2-ethanediol

    Institute of Scientific and Technical Information of China (English)

    YU,An-Guang; WANG,Nai-Xing; ZHANG,Jun-Ping; YANG,Yun-Xu; WANG,Wu-Wei; SHENG,Rui-Long

    2004-01-01

    @@ Benzopyran compounds possess diverse pharmacological properties such as β-blockade, anticonvulsant and antimicrobial.[1,2] Our interest has been focused on the synthesis of 1-[6-Fluoro-2S]-3H,4H-dihydro-2H-2-chromenyl]-(1R)-1,2-ethanediol (6) and 1-[6-fluoro-(2R)-3H,4H-dihydro-2H-2-chromenyl]-(1R)-1,2-ethanediol (7) which are particularly convenient precursor to (S,R,R,R)-NE (8). 8 containing four asymmetrical carbon atoms was reported to be the most active isomer.[3] Chandrasekhar[4] has reported on the synthesis of 8. The key step to synthesize this compound is to obtain the chiral chromanone 6 and 7. 6 was accomplished in 8 steps by the Clasien rearrangement and a one-pot Sharpless asymmetric epoxidation, but the compound 7 was accomplished in 10 steps. Johannes[5] used Zr-catalytic kinetic resolution of allylic ethers and Mo-catalyzed chromene formation to synthesize 8 in 14 steps. However both of the methods request many synthetic steps and expensive reagents.

  20. Antioxidant Activity and Isolation of Luteoline from Centaurea behen L. Grown in Iran

    Directory of Open Access Journals (Sweden)

    Akbar Esmaeili

    2013-01-01

    Full Text Available Flavonoids are secondary metabolites providing Ultraviolet-visible (UV spectroscopy protection and color in almost all terrestrial plants and fruits. They have a fused ring system consisting of an aromatic ring and a benzopyran ring with a phenyl substituent. As their biological activities have an impact on human health, they serve as target molecules in the development of new drugs. The objective of this research was to study the antioxidant activity and chemical analysis of the luteoline from Centaurea behen L. (Compositae family. The aerial parts of powdered and dried C. behen were extracted with methanol (MeOH in a Soxhlet apparatus over a period of 2 days. The concentrated total extract was extracted with petroleum ether, diethylether, and methanol. From the methanol extract of the aerial parts of C. behen, the flavonoid derivative (luteoline was identified. The aerial parts’ extract demonstrated effective antioxidant activity measured in terms of half-maximal inhibitory concentration (IC50. The product extract has been isolated by UV, column chromatography (CC, and preparative high-performance liquid chromatography (HPLC. The structures involved were elucidated by 1H and 13C nuclear magnetic resonance (NMR and heteronuclear multiple-bond correlation (HMBC spectra. The compound identified had not been reported in previous studies of C. behen L.

  1. Mikania glomerata: Phytochemical, Pharmacological, and Neurochemical Study

    Directory of Open Access Journals (Sweden)

    Lorena C. L. R. Santana

    2014-01-01

    Full Text Available The present study primarily aims to identify the relative density and the fatty acids (methyl esters content present in the standardized ethanol extract of leaves of M. glomerata (EPMG. Meanwhile, in a second moment, this study evaluated the effects of the EPMG on the levels of amino acids in the hippocampus, and the mechanism of sedative and anxiolytic action. Adult mice were treated with doses of 200, 300, and 400 mg/kg and evaluated in open field, elevated plus-maze, light dark, and rotarod tests. Moreover, in the behavioral tests diazepam (GABAergic anxiolytic, 2 mg/kg as positive control and flumazenil (GABA antagonist, 2.5 mg/kg were used to identify mechanism of sedative and anxiolytic action produced by EPMG. The EPMG is constituted by the following compounds: methyl cinnamate, 2H-1-benzopyran-2-one, (2-hydroxyphenylmethyl propionate, (Z-methyl-hexadec-7-enoate, methyl hexadecanoate, hexadecanoic acid, (Z-methyl-octadec-9-enoate, octadecanoic acid, and squalene. This extract demonstrated anxiolytic effects, which may be mediated by GABAergic system, and was able to increase GABA levels and reduce of glutamate and aspartate concentrations in mice hippocampus, which can directly and/or indirectly assist in their anxiolytic effect. Although more studies are needed, the EPMG could represent an interesting therapeutical strategy in the treatment of anxiety.

  2. In vitro and in vivo activities of 2-aminopyrazines and 2-aminopyridines in experimental models of human African trypanosomiasis.

    Science.gov (United States)

    Vodnala, Suman K; Lundbäck, Thomas; Sjöberg, Birger; Svensson, Richard; Rottenberg, Martin E; Hammarström, Lars G J

    2013-02-01

    New drugs for the treatment of human African trypanosomiasis are urgently needed. A number of 2-aminopyrazines/2-aminopyridines were identified as promising leads following a focused screen of 5,500 compounds for Trypanosoma brucei subsp. brucei viability. Described compounds are trypanotoxic in the submicromolar range and show comparably low cytotoxicity on representative mammalian cell lines. Specifically, 6-([6-fluoro-3,4-dihydro-2H-1-benzopyran-4-yl)]oxy)-N-(piperidin-4-yl)pyrazin-2-amine (CBK201352) is trypanotoxic for T. brucei subsp. brucei, T. brucei subsp. gambiense, and T. brucei subsp. rhodesiense and is nontoxic to mammalian cell lines, and in vitro preclinical assays predict promising pharmacokinetic parameters. Mice inoculated intraperitoneally (i.p.) with 25 mg/kg CBK201352 twice daily for 10 days, starting on the day of infection with T. brucei subsp. brucei, show complete clearance of parasites for more than 90 days. Thus, CBK201352 and related analogs are promising leads for the development of novel treatments for human African trypanosomiasis.

  3. Effects of NIP-141 on K currents in human atrial myocytes.

    Science.gov (United States)

    Seki, Akiko; Hagiwara, Nobuhisa; Kasanuki, Hiroshi

    2002-01-01

    A novel benzopyran derivative, NIP-141, effectively terminates experimental atrial fibrillation in canine hearts by prolonging atrial refractoriness. However, the effects of this drug on human atrial myocytes are unknown. This experiment evaluated the effects of NIP-141 on K currents in isolated human atrial myocytes using a whole-cell voltage-clamp method. NIP-141 inhibited the transient outward current (I(to)) and the ultra-rapid delayed rectifier K current (I(Kur)), each in a dose-dependent manner, with half-maximal inhibition concentrations of 16.3 microM and 5.3 microM, respectively (n = 5). NIP-141 inhibited both K currents in a voltage- and use-independent fashion, and it preferentially blocked them in the open state and dissociated rapidly from the channel. Because both K currents contribute significantly to the repolarization of the atrial action potential, these findings suggest that NIP-141 may terminate atrial fibrillation by prolonging action potential duration.

  4. Effects of a novel class III antiarrhythmic agent, NIP-142, on canine atrial fibrillation and flutter.

    Science.gov (United States)

    Nagasawa, Hidehiko; Fujiki, Akira; Fujikura, Naoki; Matsuda, Tomoyuki; Yamashita, Toru; Inoue, Hiroshi

    2002-02-01

    The effects of a new benzopyran derivative, NIP-142, on atrial fibrillation (AF) and flutter (AFL) and on electrophysiological variables were studied in the dog. NIP-142 (3mg/kg) was administered intravenously to pentobarbital-anesthetized beagles during vagally-induced AF and during AFL induced after placement of an intercaval crush. Isolated canine atrial tissues were studied using standard microelectrode technique. NIP-142 terminated AF in 5 of 6 dogs after an increase in fibrillation cycle length (CL) and prevented reinitiation of AF in all 6 dogs. NIP-142 terminated AFL in all 6 dogs without any appreciable change in flutter CL, and prevented reinitiation of AFL in all 6 dogs. NIP-142 prolonged atrial effective refractory periods (11+/-5%, 3+/-3%, 12+/-3%, and 10+/-5% from the baseline value at basic CLs of 150, 200, 300, and 350ms, respectively) without changes in intraatrial conduction time. The prolongation of the atrial effective refractory period was greater in the presence of vagal stimulation. NIP-142 decreased action potential phase-1 notch and increased phase-2 plateau height without making any changes in the action potential duration, although it did reverse carbachol-induced shortening of the action potential duration. In conclusion, NIP-142 is effective in treating AFL and vagally-induced AF by prolonging atrial refractoriness.

  5. A review of coumarin derivatives in pharmacotherapy of breast cancer.

    Science.gov (United States)

    Musa, Musiliyu A; Cooperwood, John S; Khan, M Omar F

    2008-01-01

    The coumarin (benzopyran-2-one, or chromen-2-one) ring system, present in natural products (such as the anticoagulant warfarin) that display interesting pharmacological properties, has intrigued chemists and medicinal chemists for decades to explore the natural coumarins or synthetic analogs for their applicability as drugs. Many molecules based on the coumarin ring system have been synthesized utilizing innovative synthetic techniques. The diversity oriented synthetic routes have led to interesting derivatives including the furanocoumarins, pyranocoumarins, and coumarin sulfamates (COUMATES), which have been found to be useful in photochemotherapy, antitumor and anti-HIV therapy, and as stimulants for central nervous system, antibacterials, anti-inflammatory, anti-coagulants, and dyes. Of particular interest in breast cancer chemotherapy, some coumarins and their active metabolite 7-hydroxycoumarin analogs have shown sulfatase and aromatase inhibitory activities. Coumarin based selective estrogen receptor modulators (SERMs) and coumarin-estrogen conjugates have also been described as potential antibreast cancer agents. Since breast cancer is the second leading cause of death in American women behind lung cancer, there is a strong impetus to identify potential new drug treatments for breast cancer. Therefore, the objective of this review is to focus on important coumarin analogs with antibreast cancer activities, highlight their mechanisms of action and structure-activity relationships on selected receptors in breast tissues, and the different methods that have been applied in the construction of these pharmacologically important coumarin analogs.

  6. Brazilian Propolis Antileishmanial and Immunomodulatory Effects

    Directory of Open Access Journals (Sweden)

    Suelen Santos da Silva

    2013-01-01

    Full Text Available The antileishmanial and immunomodulatory effects of propolis collected in Botucatu, São Paulo State, Brazil, were evaluated in Leishmania (Viannia braziliensis experimental infection. The antileishmanial effect of propolis on promastigote forms was verified by reducing growth and by promoting morphologic alterations observed by scanning electron microscopy. In in vitro immunomodulatory assays, macrophages were pretreated with propolis and then infected with L. (V. braziliensis. In vivo, supernatants from liver cells and peritoneal exudate of BALB/c mice pretreated with propolis and infected with Leishmania (107/mL promastigotes were collected, and TNF-α and IL-12 were measured by ELISA. Macrophages incubated with propolis showed a significant increase in interiorization and further killing of parasites. An increased TNF-α production was seen in mice pretreated with propolis, whereas IL-12 was downregulated during the infection. In conclusion, Brazilian propolis showed a direct action on the parasite and displayed immunomodulatory effects on murine macrophages, even though the parasite has been reported to affect the activation pathways of the cell. The observed effects could be associated with the presence of phenolic compounds (flavonoids, aromatic acids, and benzopyranes, di- and triterpenes, and essential oils found in our propolis sample.

  7. Antifungal dimeric chalcone derivative kamalachalcone E from Mallotus philippinensis.

    Science.gov (United States)

    Kulkarni, Roshan R; Tupe, Santosh G; Gample, Suwarna P; Chandgude, Macchindra G; Sarkar, Dhiman; Deshpande, Mukund V; Joshi, Swati P

    2014-01-01

    From the red coloured extract (Kamala) prepared through acetone extraction of the fresh whole uncrushed fruits of Mallotus philippinensis, one new dimeric chalcone (1) along with three known compounds 1-(5,7-dihydroxy-2,2,6-trimethyl-2H-1-benzopyran-8-yl)-3-phenyl-2-propen-1-one (2), rottlerin (3) and 4'-hydroxyrottlerin (4) were isolated. The structure of compound 1 was elucidated by 1D and 2D NMR analyses that included HSQC, HMBC, COSY and ROESY experiments along with the literature comparison. Compounds 1-4 were evaluated for antifungal activity against different human pathogenic yeasts and filamentous fungi. The antiproliferative activity of the compounds was evaluated against Thp-1 cell lines. Compounds 1 and 2 both exhibited IC50 of 8, 4 and 16 μg/mL against Cryptococcus neoformans PRL518, C. neoformans ATCC32045 and Aspergillus fumigatus, respectively. Compound 4, at 100 μg/mL, showed 54% growth inhibition of Thp-1 cell lines.

  8. Chalcone derivatives from the fern Cyclosorus parasiticus and their anti-proliferative activity.

    Science.gov (United States)

    Wei, Han; Zhang, Xuenong; Wu, Guanghua; Yang, Xian; Pan, Songwei; Wang, Yanyan; Ruan, Jinlan

    2013-10-01

    Three new chalcone derivatives, named parasiticins A-C (1-3), were isolated from the leaves of Cyclosorus parasiticus, together with four known chalcones, 5,7-dihydroxy-4-phenyl-8-(3-phenyl-trans-acryloyl)-3,4-dihydro-1-benzopyran-2-one (4), 2'-hydroxy-4',6'-dimethoxychalcone (5), 2',4'-dihydroxy-6'-methoxy-3',5'-dimethylchalcone (6), 2',4'-dihydroxy-6'-methoxy-3'-methylchalcone (7). The chemical structures of the new isolated compounds were elucidated unambiguously by spectroscopic data analysis. The cytotoxic activities of compounds 1-7 were evaluated against six human cancer cell lines in vitro. Compounds 3 and 6 exhibited substantial cytotoxicity against all six cell lines, especially toward HepG2 with the IC₅₀ values of 1.60 and 2.82 μM, respectively. Furthermore, we demonstrated that compounds 3 and 6 could induce apoptosis in the HepG2 cell line, which may contribute significantly to their cytotoxicity.

  9. UV-responsive polyvinyl alcohol nanofibers prepared by electrospinning

    Science.gov (United States)

    Khatri, Zeeshan; Ali, Shamshad; Khatri, Imran; Mayakrishnan, Gopiraman; Kim, Seong Hun; Kim, Ick-Soo

    2015-07-01

    We report UV-responsive polyvinyl alcohol (PVA) nanofibers for potential application for recording and erasing quick response (QR) codes. We incorporate 1‧-3‧-dihydro-8-methoxy-1‧,3‧,3‧-trimethyl-6-nitrospiro [2H-1-benzopyran-2,2‧-(2H)-indole] (indole) and,3-dihydro-1,3,3-trimethylspiro [2H-indole-2,3‧-[3H] phenanthr [9,10-b] (1,4) oxazine] (oxazine) into PVA polymer matrix via electrospinning technique. The resultant nanofibers were measured for recording-erasing, photo-coloration and thermal reversibility. The rate of photo-coloration of PVA-indole nanofibers was five times higher than the PVA-oxazine nanofibers, whereas the thermal reversibility found to be more than twice as fast as PVA-oxazine nanofibers. Results showed that the resultant nanofibers have very good capability of recording QR codes multiple times. The FTIR spectroscopy and SEM were employed to characterize the electrospun nanofibers. The UV-responsive PVA nanofibers have great potentials as a light-driven nanomaterials incorporated within sensors, sensitive displays and in optical devices such as erasable and rewritable optical storage.

  10. Puerarin accelerates neural regeneration after sciatic ner ve injur y

    Institute of Scientific and Technical Information of China (English)

    Minfei Wu; Guanjie Zhao; Xiaoyu Yang; Chuangang Peng; Jianwu Zhao; Jun Liu; Rui Li; Zhongli Gao

    2014-01-01

    Puerarin is a natural isolfavone isolated from plants of the genus Pueraria and functions as a protector against cerebral ischemia. We hypothesized that puerarin can be involved in the repair of peripheral nerve injuries. To test this hypothesis, doses of 10, 5, or 2.5 mg/kg per day puer-arin (8-(β-D-Glucopyranosyl-7-hydroxy-3-(4-hydroxyphenyl)-4H-1-benzopyran-4-one) were injected intraperitoneally into mouse models of sciatic nerve injury. Puerarin at the middle and high doses signiifcantly up-regulated the expression of growth-associated protein 43 in the L4-6 segments of the spinal cord from mice at 1, 2, and 4 weeks after modeling, and reduced the atro-phy of the triceps surae on the affected side and promoted the regeneration of nerve ifbers of the damaged spinal cord at 8 weeks after injury. We conclude that puerarin exerts an ongoing role to activate growth-associated protein 43 in the corresponding segment of the spinal cord after sciat-ic nerve injury, thus contributing to neural regeneration after sciatic nerve injuries.

  11. Anti-Trichomonas vaginalis activity of Hypericum polyanthemum extract obtained by supercritical fluid extraction and isolated compounds.

    Science.gov (United States)

    Cargnin, Simone Tasca; Vieira, Patrícia de Brum; Cibulski, Samuel; Cassel, Eduardo; Vargas, Rubem Mário Figueiró; Montanha, Jarbas; Roehe, Paulo; Tasca, Tiana; von Poser, Gilsane Lino

    2013-04-01

    The anti-Trichomonas vaginalis activity of Hypericum polyanthemum extract obtained by supercritical fluid extraction (50°C, 150bar) and the chemical compounds isolated and purified from this extract (benzopyrans HP1, HP2, HP3, and phloroglucinol derivative uliginosin B) were assessed. All samples had anti-T. vaginalis activity; however, HP1 demonstrated the best selectivity against this protozoan (metronidazole-resistant and susceptible isolates), with no cytotoxicity on mammalian cells (selectivity index of 73.97). Moreover, HP1 had activity against a metronidazole-resistant isolate (52% of viable trophozoites), and this effect was higher when tested with a low concentration of metronidazole (23% of viable trophozoites). Experiments demonstrated that all isolated compounds caused damage to the parasites' membrane (>90% of LDH release) and do not present a notable hemolytic effect, although HP2 and uliginosin B exhibited cytotoxicity against mammalian cells. Therefore, the analyzed molecules are promising prototypes for new antiprotozoal drugs, especially HP1, which seems to improve metronidazole's effect on a resistant T. vaginalis isolate.

  12. Crystal Structure of 3S-hydroxy-7 Melleine

    Science.gov (United States)

    Liu, Xiao-hong; Xu, Fang; Zhang, Yi; Liu, Li-hong; Huang, Hua-rong; She, Zhi-gang; Lin, Yong-cheng; Chan, Winglai

    2006-10-01

    A new compound, 3S-hydroxy-7 melleine was isolated from the endophytic fungus Xylariasp No.2508 from the mangrove tree on the South China Sea coast. It was the first time that this kind of compound was isolated from marine fungus. The structure was elucidated by NMR data, infrared spectrum (IR) and mass spectrometry (MS). In addition, its structure was determined by the single-crystal X-ray diffraction analysis. It crystallized in monoclinic, space group P21 with a=10.8884(19) Å, b=7.2284(13) Å, c=13.398(2) Å, β=104.217(3)°, C10H10O4·H2O, Mr=212.20, V=1022.2(3) Å3, Z=4, Dc=1.379 mg/m3, F(000)=448, μ=0.112 mm-1, the final R=0.0498, ωR=0.101 for 2407 observed reflections (I>2σ(I)). The molecular backbone of the compound includes a benzopyran ring. By comparing with the melting point and the optical rotation of the known 3R-hydroxy-7 melleine in literature, the absolute configuration of the compound was determined as 3S. It didn't exhibit antibacterial activity against Gram-positive bacterium Staphylococcus aureus at 200 μg/disk in the preliminary test.

  13. PM-3, a benzo-gamma-pyran derivative isolated from propolis, inhibits growth of MCF-7 human breast cancer cells.

    Science.gov (United States)

    Luo, J; Soh, J W; Xing, W Q; Mao, Y; Matsuno, T; Weinstein, I B

    2001-01-01

    Propolis has numerous biologic activities including antibiotic, antifungal, antiviral and anti-inflammatory properties. Several components isolated from propolis have been shown to have anticancer activity. This study demonstrates that the compound PM-3 (3-[2-dimethyl-8-(3-methyl-2-butenyl)benzopyran]-6-propenoic acid) isolated from Brazilian propolis markedly inhibits the growth of MCF-7 human breast cancer cells. This effect was associated with inhibition of cell cycle progression and induction of apoptosis. Treatment of MCF-7 cells with PM-3 arrested cells in the G1 phase and resulted in a decrease in the protein levels of cyclin D1 and cyclin E. PM-3 also inhibited the expression of cyclin D1 at the transcriptional level when examined in cyclin D1 promoter luciferase assays. Induction of apoptosis by PM-3 occurred within 48 hours after treatment of MCF-7 cells. The MCF-7 treated cells also displayed a decrease in the level of the estrogen receptor (ER) protein and inhibition of estrogen response element (ERE) promoter activity. Therefore, PM-3 merits further investigation with respect to breast cancer chemoprevention or therapy.

  14. [A new monoterpenoid glucoside from the twigs of Chamaecyparis obtusa var. breviramea f. crippsii].

    Science.gov (United States)

    Xu, Jian; Zhang, Yu-Mei; Chen, Ke-Li; Tan, Ning-Hua; Liu, Yi-Mei

    2012-09-01

    To investigate the chemical constituents of Chamaecyparis obtusa var. breviramea f. crippsii, various column chromatography and spectroscopic methods were used for the isolation and elucidation of compounds. One new monoterpenoid glucoside, (4S)-4-isopropylcyclohex-l-enecarboxylic acid 4-O-beta-D-glucopyranoside (1), together with five known compounds, (4R)-p-menth-l-ene-7, 8-diol 7-O-beta-D-glucopyranoside (2), skimmin (3), 7-[[6-O-(6-deoxy-alpha-L-mannopyranosyl)-beta-D-glucopyranosyl]oxy]-2H-1-benzopyran-2-one (4), stigmast-4-en-3-one (5) and 1, 4-benzenedicarboxylic acid 1-butyl-4-(2-methylpropyl) ester (6) were isolated and identified from the twigs of this plant. All compounds were isolated from this plant for the first time. The methanol extract of this plant showed cytotoxicity on cancer cell lines A549, BGC-823, Du145 and MDA-MB-231 with IC50 values of 0.94, 1.07, 0.95 and 0.96 microg x mL(-1), respectively. Yet, compounds 1, 2 and 3 showed no cytotoxicity on cancer cell lines HeLa, BGC-823 and A549.

  15. Photochromic glass thin film formed by the sol-gel coating method

    Science.gov (United States)

    Nakazumi, Hiroyuki; Nagashiro, Rie; Matsumoto, Shinya; Isagawa, Kakuzoh

    1994-10-01

    The photochromic gel thin films using 1'-butyl-3',3'-dimethyl-6-nitro-spiro[2H-1- benzopyran-2,2'-indoline] (1) and 1'-butyl-spiro[2H-indole-2,3'- [3H]naphtho[2,1-b][1,4]oxazine] (2) dispersed in sol in the sol-gel processing were prepared and photochromic behaviors of these films were investigated. A good transparent coating layer on glass surface was formed in the range of ca. 6 - 10 wt% of 1 or 2 to alkoxysilane, and was colored by UV irradiation. The absorption band formed by UV-irradiation disappeared by thermal decay and also by Vis irradiation for 1. The thermal fading of the colored form to the spiro form 1 or 2 is dependent on a matrix of the gels, the colored forms in the film starting from methyltriethoxysilane (MTES), which is expected to include larger pores than in the film starting from tetraethoxysilane (TEOS) or silane oligomer, show faster thermal fading which roughly follows the first order kinetics. The colored form in the gel is stabilized, compared with that in solution or bulk gel, and it is suggested that there are some kinds of colored species in thin gel films containing spiropyran 1, which may be some aggregates, whereas only a colored species from spironaphthooxazine 2 is suggested. Photochromic behavior of 2 in sol was also examined.

  16. Identification, isolation and characterization of the antifeedant constituent of Clausena anisata against Helicoverpa armigera (Lepidoptera: Noctuidae)

    Institute of Scientific and Technical Information of China (English)

    Olufemi O.R.Pitan; O.O.Ayelaagbe; Hong-Lei Wang; Chen-Zhu Wang

    2009-01-01

    Hexane, petroleum ether, chloroform, ethyl acetate, methanol and water extracts of Clausena anisata [(Willd.) Hook F. Ex Benth] leaves and roots were evaluated against Helicoverpa armigera (Hubner) for antifeedant activities. Antifeedant activity was confirmed, and was found to be higher in root extracts than those of the leaf. Chloroform and petroleum ether extracts of the root showed strongest antifeedant activities (DC50S [concentration (C) causing 50% deterrence compared with the control] 0.014% and 0.016% respectively), and root extracts were fractionated using silica gel column chromatography. One fraction of the chloroform and one of the petroleum ether root extracts was active; and on the basis of mass spectroscopy and 1H and 13C nuclear magnetic resonance spectral data, the active compounds in the two fractious were confirmed to be identical, and identified as osthol [2H-I-Benzopyran-2-one, 7-methoxy-8-(3-methyl-2-butenyl)]. The highest concentratiun of osthol was found in the chloroform root extract. Antifeedant activities of the root extracts, as measured by DC50 values, were highly correlated with their osthol contents. Approximately 99% of the variation in bioactivity of the root extracts could be accounted for by variation in osthol content; osthol therefore, appeared to be an antifeedant component of C. Anisata to H. Armigera. This may provide a new approach to managing this pest.

  17. 4H-[1]苯并吡喃[3,4-d]异噁唑-4-酮的合成%Synthesis of 4H-[1] Benzopyrano [3,4-d]isoxazol-4-ones

    Institute of Scientific and Technical Information of China (English)

    邓兴国; 邢媛媛; 王有名; 周正洪

    2012-01-01

    芳酰乙酸乙酯在乙氧基镁的作用下与取代的苯甲酰氯反应生成2-取代苯并吡喃酮.2-取代苯并吡喃酮和盐酸羟胺经Beckmann重排反应合成了4H-[1]苯并吡喃[3,4-d]异噁唑-4-酮.X衍射谱图表明,反应过程中表现区域选择性.对该反应的反应机理进行了初探.%Ethyl benzoylacetate reacted with acetylsalicyloyl chloride in the presence of magnesium ethylate and ethyl 2-ary1-4-oxo-4H-[1]benzopyran-3-carbonate were gained,which reacted with hydroxylamine via Beckmann rearrangement and 4H-[1]benzopyrano [3,4-d]isoxazol-4-ones were gained.X-Ray analysis result of compound 2-b confirmed the regioselectivity of the reaction.The mechanism of the formation of 4H-[1]benzopyrano[3,4-d]isoxazol-4-ones is also investigated.

  18. Secondary metabolites from Ganoderma.

    Science.gov (United States)

    Baby, Sabulal; Johnson, Anil John; Govindan, Balaji

    2015-06-01

    Ganoderma is a genus of medicinal mushrooms. This review deals with secondary metabolites isolated from Ganoderma and their biological significance. Phytochemical studies over the last 40years led to the isolation of 431 secondary metabolites from various Ganoderma species. The major secondary compounds isolated are (a) C30 lanostanes (ganoderic acids), (b) C30 lanostanes (aldehydes, alcohols, esters, glycosides, lactones, ketones), (c) C27 lanostanes (lucidenic acids), (d) C27 lanostanes (alcohols, lactones, esters), (e) C24, C25 lanostanes (f) C30 pentacyclic triterpenes, (g) meroterpenoids, (h) farnesyl hydroquinones (meroterpenoids), (i) C15 sesquiterpenoids, (j) steroids, (k) alkaloids, (l) prenyl hydroquinone (m) benzofurans, (n) benzopyran-4-one derivatives and (o) benzenoid derivatives. Ganoderma lucidum is the species extensively studied for its secondary metabolites and biological activities. Ganoderma applanatum, Ganoderma colossum, Ganoderma sinense, Ganoderma cochlear, Ganoderma tsugae, Ganoderma amboinense, Ganoderma orbiforme, Ganoderma resinaceum, Ganoderma hainanense, Ganoderma concinna, Ganoderma pfeifferi, Ganoderma neo-japonicum, Ganoderma tropicum, Ganoderma australe, Ganoderma carnosum, Ganoderma fornicatum, Ganoderma lipsiense (synonym G. applanatum), Ganoderma mastoporum, Ganoderma theaecolum, Ganoderma boninense, Ganoderma capense and Ganoderma annulare are the other Ganoderma species subjected to phytochemical studies. Further phytochemical studies on Ganoderma could lead to the discovery of hitherto unknown biologically active secondary metabolites.

  19. Flavonoids and the CNS

    Directory of Open Access Journals (Sweden)

    Anna K. Jäger

    2011-02-01

    Full Text Available Flavonoids are present in almost all terrestrial plants, where they provide UV-protection and colour. Flavonoids have a fused ring system consisting of an aromatic ring and a benzopyran ring with a phenyl substituent. The flavonoids can be divided into several classes depending on their structure. Flavonoids are present in food and medicinal plants and are thus consumed by humans. They are found in plants as glycosides. Before oral absorption, flavonoids undergo deglycosylation either by lactase phloridzin hydrolase or cytosolic β-glucocidase. The absorbed aglycone is then conjugated by methylation, sulphatation or glucuronidation. Both the aglycones and the conjugates can pass the blood-brain barrier. In the CNS several flavones bind to the benzodiazepine site on the GABAA-receptor resulting in sedation, anxiolytic or anti-convulsive effects. Flavonoids of several classes are inhibitors of monoamine oxidase A or B, thereby working as anti-depressants or to improve the conditions of Parkinson’s patients. Flavanols, flavanones and anthocyanidins have protective effects preventing inflammatory processes leading to nerve injury. Flavonoids seem capable of influencing health and mood.

  20. In Vivo Metabolism Study of Xiamenmycin A in Mouse Plasma by UPLC-QTOF-MS and LC-MS/MS

    Directory of Open Access Journals (Sweden)

    Feng Lei

    2015-01-01

    Full Text Available Xiamenmycin A is an antifibrotic leading compound with a benzopyran skeleton that is isolated from mangrove-derived Streptomyces xiamenensis. As a promising small molecule for fibrotic diseases, less information is known about its metabolic characteristics in vivo. In this study, the time-course of xiamenmycin A in mouse plasma was investigated by relative quantification. After two types of administration of xiamenmycin A at a single dose of 10 mg/kg, the plasma concentrations were measured quantitatively by LC-MS/MS. The dynamic changes in the xiamenmycin A concentration showed rapid absorption and quick elimination in plasma post-administration. Four metabolites (M1–M4 were identified in blood by UPLC-QTOF-MS, and xiamenmycin B (M3 is the principal metabolite in vivo, as verified by comparison of the authentic standard sample. The structures of other metabolites were identified based on the characteristics of their MS and MS/MS data. The newly identified metabolites are useful for understanding the metabolism of xiamenmycin A in vivo, aiming at the development of an anti-fibrotic drug candidate for the therapeutic treatment of excessive fibrotic diseases.

  1. Neuroprotective actions of flavones and flavonols: mechanisms and relationship to flavonoid structural features.

    Science.gov (United States)

    Dajas, Federico; Andrés, Abin-Carriquiry Juan; Florencia, Arredondo; Carolina, Echeverry; Felicia, Rivera-Megret

    2013-03-01

    Epidemiological studies have shown positive preventive action of flavonoids on cardiovascular and neurodegenerative events. Among the six groups in which flavonoids are classified, the flavones and flavonols, based on the backbone of 2-phenylchromen-4-one (2-phenyl-1-benzopyran-4-one) are the most commonly encountered within the families and genera of the higher plants. Numerous studies support a neuroprotective activity of flavones such as luteolin and flavonols such as kaempherol and quercetin in experimental focal ischemia and models of neurodegeneration. Antioxidation, modulation of signaling cascades and gene expression as well as anti-inflammation appear as the main protective mechanisms and mitochondria are a likely main target mediating the preventive actions against oxidative stress. Flavones and flavonols re-establish the redox regulation of proteins, transcription factors and signaling cascades that are otherwise inhibited by elevated oxidative stress. The final survival or death of the neuron depends on flavone and flavonol concentrations, time of exposure and, mainly, metabolic and oxidative neuronal circumstances. Neuroprotection appears to be linked to specific structural motifs, beyond those involved in antioxidation. By themselves or as templates for synthetic compounds, flavone and flavonol molecules show potential as multi-targeted therapeutic tools for protecting the brain. Nonetheless, more research needs to be done on the correlation of potential beneficial effects of flavones and flavonols and their mechanisms of action.

  2. A new monoterpenoid glucoside from the twigs of Chamaecyparis obtusa var.breviramea f.crippsii%黄叶扁柏枝叶中的一个新单萜苷

    Institute of Scientific and Technical Information of China (English)

    徐健; 张玉梅; 陈科力; 谭宁华; 刘义梅

    2012-01-01

    为了研究黄叶扁柏(Chamaecyparis obtusa var.breviramea f.crippsii)的化学成分,用硅胶柱、Sephadex LH-20、C18反相硅胶柱色谱、制备液相等色谱方法从其枝叶中分离得到6个化合物,通过波谱方法将它们的结构分别鉴定为(4S)-4-isopropylcyclohex-1-enecarboxylic acid 4-O-β-D-glucopyranoside (1)、(4R)-p-menth- 1-ene-7,8-diol 7-O-β-D-glucopyranoside (2)、skimmin (3)、7-[[6-O-(6-deoxy-α-L-mannopyranosyl)-β-D-glucopyranosyl]oxy]-2H-1-benzopyran-2-one (4)、stigmast-4-en-3-one (5)和1,4-benzenedicarboxylic acid l-butyl-4-(2-methylpropyl) ester (6).其中化合物1为新化合物,化合物2~6为首次从该植物中分离得到.体外细胞毒活性筛选发现,黄叶扁柏枝叶的甲醇提取物在人A549、BGC-823、Du145和MDA-MB-231肿瘤细胞株上均显示一定的细胞毒活性,其IC50值分别为0.94、1.07、0.95和0.96 μg·mL-1,但化合物1、2和3在人HeLa、BGC-823和A549细胞株上均无明显细胞毒活性.%To investigate the chemical constituents of Chamaecyparis obtusa var. breviramea f. crippsii, various column chromatography and spectroscopic methods were used for the isolation and elucidation of compounds. One new monoterpenoid glucoside, (4S)-4-isopropylcyclohex-l-enecarboxylic acid 4-O-β-D-glucopyranoside (1), together with five known compounds, (4R)-p-menth-l-ene-7, 8-diol 7-O-β-D-glucopyranoside (2), skimmin (3), 7-[[6-O-(6-deoxy-α-Z-mannopyranosyl)-β-Z)-glucopyranosyl]oxy]-2H-1-benzopyran-2-one (4), stigmast-4-en-3-one (5) and 1, 4-benzenedicarboxylic acid l-butyl-4-(2-methylpropyl) ester (6) were isolated and identified from the twigs of this plant. All compounds were isolated from this plant for the first time. The methanol extract of this plant showed cytotoxicity on cancer cell lines A549, BGC-823, Dul45 and MDA-MB-231 with IC50 values of 0.94, 1.07, 0.95 and 0.96 μg·mL-1, respectively. Yet, compounds 1, 2 and 3 showed no cytotoxicity on cancer cell lines HeLa, BGC-823 and A549.

  3. Identification of catechin as one of the flavonoids from Combretum albiflorum bark extract that reduces the production of quorum-sensing-controlled virulence factors in Pseudomonas aeruginosa PAO1.

    Science.gov (United States)

    Vandeputte, Olivier M; Kiendrebeogo, Martin; Rajaonson, Sanda; Diallo, Billo; Mol, Adeline; El Jaziri, Mondher; Baucher, Marie

    2010-01-01

    Quorum-sensing (QS) regulates the production of key virulence factors in Pseudomonas aeruginosa and other important pathogenic bacteria. In this report, extracts of leaves and bark of Combretum albiflorum (Tul.) Jongkind (Combretaceae) were found to quench the production of QS-dependent factors in P. aeruginosa PAO1. Chromatographic fractionation of the crude active extract generated several active fractions containing flavonoids, as shown by their typical spectral features. Purification and structural characterization of one of the active compounds led to the identification of the flavan-3-ol catechin [(2R,3S)-2-(3,4-dihydroxyphenyl)-3,4-dihydro-1(2H)-benzopyran-3,5,7-triol]. The identity of catechin as one of the active molecules was confirmed by comparing the high-pressure liquid chromatography profiles and the mass spectrometry spectra obtained for a catechin standard and for the active C. albiflorum fraction. Moreover, standard catechin had a significant negative effect on pyocyanin and elastase productions and biofilm formation, as well as on the expression of the QS-regulated genes lasB and rhlA and of the key QS regulatory genes lasI, lasR, rhlI, and rhlR. The use of RhlR- and LasR-based biosensors indicated that catechin might interfere with the perception of the QS signal N-butanoyl-l-homoserine lactone by RhlR, thereby leading to a reduction of the production of QS factors. Hence, catechin, along with other flavonoids produced by higher plants, might constitute a first line of defense against pathogenic attacks by affecting QS mechanisms and thereby virulence factor production.

  4. Kaempferol, a potential cytostatic and cure for inflammatory disorders.

    Science.gov (United States)

    Rajendran, Peramaiyan; Rengarajan, Thamaraiselvan; Nandakumar, Natarajan; Palaniswami, Rajendran; Nishigaki, Yutaka; Nishigaki, Ikuo

    2014-10-30

    Kaempferol (3,5,7-trihydroxy-2-(4-hydroxyphenyl)-4H-1-benzopyran-4-one) is a flavonoid found in many edible plants (e.g., tea, broccoli, cabbage, kale, beans, endive, leek, tomato, strawberries, and grapes) and in plants or botanical products commonly used in traditional medicine (e.g., Ginkgo biloba, Tilia spp, Equisetum spp, Moringa oleifera, Sophora japonica and propolis). Its anti-oxidant/anti-inflammatory effects have been demonstrated in various disease models, including those for encephalomyelitis, diabetes, asthma, and carcinogenesis. Moreover, kaempferol act as a scavenger of free radicals and superoxide radicals as well as preserve the activity of various anti-oxidant enzymes such as catalase, glutathione peroxidase, and glutathione-S-transferase. The anticancer effect of this flavonoid is mediated through different modes of action, including anti-proliferation, apoptosis induction, cell-cycle arrest, generation of reactive oxygen species (ROS), and anti-metastasis/anti-angiogenesis activities. In addition, kaempferol was found to exhibit its anticancer activity through the modulation of multiple molecular targets including p53 and STAT3, through the activation of caspases, and through the generation of ROS. The anti-tumor effects of kaempferol have also been investigated in tumor-bearing mice. The combination of kaempferol and conventional chemotherapeutic drugs produces a greater therapeutic effect than the latter, as well as reduces the toxicity of the latter. In this review, we summarize the anti-oxidant/anti-inflammatory and anticancer effects of kaempferol with a focus on its molecular targets and the possible use of this flavonoid for the treatment of inflammatory diseases and cancer.

  5. Potato wound-healing tissues: A rich source of natural antioxidant molecules with potential for food preservation.

    Science.gov (United States)

    Dastmalchi, Keyvan; Wang, Isabel; Stark, Ruth E

    2016-11-01

    The need for safe, effective preservatives is a prominent issue in the food and drug industries, reflecting demand for natural alternatives to synthetic chemicals viewed as harmful to consumers and the environment. Thus, this study determined the identities and scavenging capacities of antioxidant metabolites produced as a response to potato tuber wounding, using activity-guided fractionation of polar extracts from a Yukon Gold cultivar that had previously exhibited exceptionally high radical-scavenging activity. Activity-guided fractionation using the ABTS(+) radical scavenging assay and LC-MS with TOF-MS for compositional analysis of the most potent antioxidant fractions yielded identification of nine constituents: coumaroylputrescine; feruloylquinic acid; isoferuloylputrescine; ferulic acid; 22,25-dimethoxy-3-[[2,3,4-tri-O-methyl-6-O-(2,3,4,6-tetra-O-methyl-β-d-glucopyranosyl)-β-d-glucopyranosyl]oxy]-(3β)-lanost-9(11)-en-24-one; 4-(2Z)-2-decen-1-yl-5-[1-(4-hydroxyphenyl)decyl]-1,2-benzenediol; 8-[(2E)-3,7-dimethyl-2,6-octadien-1-yl]-5-hydroxy-2,8-dimethyl-6-(3-methyl-2-buten-1-yl)-2H-1-benzopyran-4,7(3H,8H)-dione; 3-[(2-O-β-d-glucopyranosyl-β-d-glucopyranosyl)oxy]-20-[(6-O-β-d-xylopyranosyl-β-d-glucopyranosyl)oxy]-dammar-24-en-19-al; (3β)-28-oxo-28-(phenylmethoxy)oleanan-3-yl 2-O-β-d-galactopyranosyl-3-O-(phenylmethyl)-, butyl ester β-d-glucopyranosiduronic acid. A positive correlation was observed between the scavenging activities and the polarities of the active fractions. The antioxidant capacities of the fractions were also characterised by monitoring the activity throughout a 45-minute assay period.

  6. Involvement of gap junction channels in the pathophysiology of migraine with aura

    Directory of Open Access Journals (Sweden)

    Denis eSarrouilhe

    2014-02-01

    Full Text Available Migraine is a common, recurrent and disabling primary headache disorder with a genetic component which affects up to 20 % of the population. One third of all patients with migraine experiences aura, a focal neurological disturbance that manifests itself as visual, sensitive or motor symptoms preceding the headache. In the pathophysiology of migraine with aura, activation of the trigeminovascular system from the meningeal vessels mediates migraine pain via the brainstem and projections ascend to the thalamus and cortex. Cortical spreading depression (CSD was proposed to trigger migraine aura and to activate perivascular trigeminal nerves in the cortex. Quinine, quinidine and the derivative mefloquine are able to inhibit CSD suggesting an involvement of neuronal connexin36 channels in CSD propagation. More recently, CSD was shown to induce headache by activating the trigeminovascular system through the opening of stressed neuronal Pannexin1 channels. A novel benzopyran compound, tonabersat, was selected for clinical trial on the basis of its inhibitory activity on CSD and neurogenic inflammation in animal models of migraine. Interestingly, in the time course of animal model trials, tonabersat was shown to inhibit trigeminal ganglion neuronal-glial cell gap junctions, suggesting that this compound could prevent peripheral sensitization within the ganglion. Three clinical trials aimed at investigating the effectiveness of tonabersat as a preventive drug were negative, and conflicting results were obtained in other trials concerning its ability to relieve attacks. In contrast, in another clinical trial, tonabersat showed a preventive effect on attacks of migraine with aura but had no efficacy on non-aura attacks. Gap junction channels seem to be involved in several ways in the pathophysiology of migraine with aura and emerge as a new promising putative target in treatment of this disorder.

  7. Levels of synthetic musk fragrances in human milk from three cities in the Yangtze River Delta in Eastern China.

    Science.gov (United States)

    Zhang, Xiaolan; Liang, Gaofeng; Zeng, Xiangying; Zhou, Jing; Sheng, Guoying; Ful, Jiamo

    2011-01-01

    Synthetic musks are used as additives in many household products. After absorption into the human body, they accumulate and their concentrations in human milk reflect both the mother and her infant's exposure level. Concentrations of four synthetic musks, musk xylene (1-tert-butyl-2,6-dimethyl-2,4,6-trinitrobenzene, MX), musk ketone (4-tert-butyl-2,6-dimethyl-3,5-dinitroacetophenone, MK), 1,3,4,6,7,8-hexahydro-4,6,6,7,8,8-hexamethylcyclopenta[gamma]-2-benzopyran (HHCB) and 7-acetyl-1,1,3,4,4,6-hexamethyl- 1,2,3,4-tetrahydronaphthalene (AHTN), were determined in human milk samples collected from Shanghai, Wuxi, and Shaoxing in Eastern China. The four synthetic musks were found in most samples analyzed, with HHCB the dominant component followed by MX. The median (mean) values for HHCB, AHTN, MX and MK concentrations were 63 (82), 5 (12), 17 (24) and 4 (9) ng/g lipid weight, respectively. These data suggested the total synthetic musk contamination was low, and the distribution percentage was HHCB > MX > AHTN approximately MK. The relative high ratio of nitro to polycylic musk indicated that nitro musks were still widely used. The musk concentrations in these cities were not significantly different from each other (p > 0.05). Principal components score plots were obtained, which showed similar exposure sources. The amount of total synthetic musks in human milk were not associated with mother's age, although HHCB was significantly correlated with AHTN (p < 0.05). Daily ingestion of HHCB, AHTN, MX and MK for infants from human milk were estimated as (2526 +/- 2926), (370 +/- 524), (7391 +/- 832), and (277 +/- 462) ng/day, respectively. Those doses were 1-2 orders of magnitude below the provisional tolerable daily intakes.

  8. A multiple ion channel blocker, NIP-142, for the treatment of atrial fibrillation.

    Science.gov (United States)

    Tanaka, Hikaru; Hashimoto, Norio

    2007-01-01

    Atrial fibrillation (AF) is one of the most frequent cardiac arrhythmia and is associated with increased cardiovascular morbidity and mortality, and the risk of stroke. Although currently available antiarrhythmic drugs are moderately effective in restoring normal sinus rhythm in patients with AF, excessive delay of ventricular repolarization by these agents may be associated with increased risk of proarrhythmia. Therefore, selective blockers of cardiac ion channel(s) that are exclusively present in the atria are highly desirable. NIP-142 is a novel benzopyrane derivative, which blocks potassium, calcium, and sodium channels and shows atrial specific action potential duration prolongation. NIP-142 preferentially blocks the ultrarapid delayed rectifier potassium current (I Kur) and the acetylcholine-activated potassium current (I KACh). Since I Kur and I KACh have been shown to be expressed more abundantly in the atrial than in the ventricular myocardium, the atrial-specific repolarization prolonging effect of NIP-142 is considered to be due to the blockade of these potassium currents. In canine models, NIP-142 was shown to terminate the microreentry type AF induced by vagal nerve stimulation and the macroreentry type atrial flutter induced by an intercaval crush. These effects of NIP-142 have been attributed to the prolongation of atrial effective refractory period (ERP), because this compound prolonged atrial ERP without affecting intraatrial and interatrial conduction times in these models. The ERP prolongation by NIP-142 was greater in the atrium than in the ventricle. NIP-142 also terminated the focal activity type AF induced by aconitine. In addition, NIP-142 reversed the atrial ERP shortening and the loss of rate adaptation induced by short-term rapid atrial pacing in anesthetized dogs. Thus, although clinical trials are required to provide evidence for its efficacy and safety, the novel multiple ion channel blocker, NIP-142, appears to be a useful agent for

  9. Atria selective prolongation by NIP-142, an antiarrhythmic agent, of refractory period and action potential duration in guinea pig myocardium.

    Science.gov (United States)

    Matsuda, Tomoyuki; Takeda, Kentaro; Ito, Mie; Yamagishi, Reiko; Tamura, Miku; Nakamura, Hideki; Tsuruoka, Noriko; Saito, Tomoaki; Masumiya, Haruko; Suzuki, Takeshi; Iida-Tanaka, Naoko; Itokawa-Matsuda, Maho; Yamashita, Toru; Tsuruzoe, Nobutomo; Tanaka, Hikaru; Shigenobu, Koki

    2005-05-01

    NIP-142 is a novel benzopyran compound that was shown to prolong the atrial effective refractory period and terminate experimental atrial fibrillation in the dog. In the present study, we examined the effects of NIP-142 on isolated guinea pig myocardium and on the G-protein-coupled inwardly rectifying potassium channel current (acetylcholine-activated potassium current; I(KACh)) expressed in Xenopus oocytes. NIP-142 (10 and 100 microM) concentration-dependently prolonged the refractory period and action potential duration in the atrium but not in the ventricle. E-4031 and 4-aminopyridine prolonged action potential duration in both left atrium and right ventricle. Prolongation by NIP-142 of the atrial action potential duration was observed at stimulation frequencies between 0.5 and 5 Hz. In contrast, the prolongation by E-4031 was not observed at higher frequencies. Tertiapin, a blocker of I(KACh), prolonged action potential duration in the atrium but not in the ventricle. NIP-142 completely reversed the carbachol-induced shortening of atrial action potential duration. NIP-142 (1 to 100 microM), as well as tertiapin (0.1 to 100 nM), concentration-dependently blocked I(KACh) expressed in Xenopus oocytes; the blockade by NIP-142 was not affected by membrane voltage. In conclusion, NIP-142 was shown to prolong atrial refractory period and action potential duration through blockade of I(KACh) which may possibly explain its previously described antiarrhythmic activity. NIP-142 has pharmacological properties that are different from classical class III antiarrhythmic agents such as atria specificity and lack of reverse frequency dependence, and thus appears promising for the treatment of supraventricular arrhythmia.

  10. Pomegranate juice ellagitannin metabolites are present in human plasma and some persist in urine for up to 48 hours.

    Science.gov (United States)

    Seeram, Navindra P; Henning, Susanne M; Zhang, Yanjun; Suchard, Marc; Li, Zhaoping; Heber, David

    2006-10-01

    Ellagitannins (ETs) from pomegranate juice (PJ) are reported to have numerous biological properties, but their absorption and metabolism in humans are poorly understood. To investigate the pharmacokinetics of pomegranate ETs, 18 healthy volunteers were given 180 mL of PJ concentrate, and blood samples were obtained for 6 h afterwards. Twenty-four-hour urine collections were obtained on the day before (-1), the day of (0), and the day after (+1) the study. Ellagic acid (EA) was detected in plasma of all subjects with a maximum concentration of 0.06 +/- 0.01 micromol/L, area under concentration time curve of 0.17 +/- 0.02 (micromol x h) x L(-1), time of maximum concentration of 0.98 +/- 0.06 h, and elimination half-life of 0.71 +/- 0.08 h. EA metabolites, including dimethylellagic acid glucuronide (DMEAG) and hydroxy-6H-benzopyran-6-one derivatives (urolithins), were also detected in plasma and urine in conjugated and free forms. DMEAG was found in the urine obtained from 15 of 18 subjects on d 0, but was not detected on d -1 or +1, demonstrating its potential as a biomarker of intake. Urolithin A-glucuronide was found in urine samples from 11 subjects on d 0 and in the urine from 16 subjects on d +1. Urolithin B-glucuronide was found in the urine of 3 subjects on d 0 and in the urine of 5 subjects on d +1. Urolithins, formed by intestinal bacteria, may contribute to the biological effects of PJ as they may persist in plasma and tissues and account for some of the health benefits noted after chronic PJ consumption. Whether genetic polymorphisms in EA-metabolizing enzymes (e.g., catechol-O-methyl transferase and glucuronosyl transferase) are related to variations in response to PJ remains to be established.

  11. Berberine protects endothelial progenitor cell from damage of TNF-α via the PI3K/AKT/eNOS signaling pathway.

    Science.gov (United States)

    Xiao, Min; Men, Li Na; Xu, Ming Guo; Wang, Guo Bing; Lv, Hai Tao; Liu, Cong

    2014-11-15

    Endothelial progenitor cells (EPCs) dysfunction is closely correlated with the coronary artery injury induced by Kawasaki disease (KD). The level of tumor necrosis factor-α (TNF-α) elevated significantly in acute phase of KD which can damage the functions of EPCs. The aim of this study was to investigate whether berberine (BBR) can protect EPCs from the inhibition caused by TNF-α via the PI3K (Phosphatidyl Inositol 3-kinase) /AKT (Serine/threonine protein kinase B) /eNOS (endothelial Nitric Oxide synthase) signaling pathway. The cell proliferative ability of EPCs was determined by MTT (methyl thiazolyl tetrazolium) assays. Nitric oxide (NO) level was determined in supernatants. The mRNA level of eNOS, PI3K and AKT were measured by Real Time-Polymerase Chain Reaction (RT-PCR), and the protein levels of eNOS, phospho-eNOS (p-eNOS), Akt, phospho-Akt (p-Akt) and PI3K were analyzed using Western-blot. The results demonstrated that TNF-α inhibits the proliferative ability of EPCs. However, BBR improves the proliferative activity of EPCs inhibited by TNF-α. Blockade of PI3K by 2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one (Ly294002) and blockade of eNOS by l-NAME (NG-Nitroarginine Methyl Ester) attenuates the effect of BBR. BBR can increase the level of PI3K/Akt/eNOS mRNA and the protein level of PI3K, p-Akt, eNOS and p-eNOS, which can be blocked by PI3K inhibitor (LY294002) and eNOS inhibitor (l-NAME). Therefore, we concluded that impaired EPCs proliferation could be reversed by BBR via the PI3K/AKT/eNOS signaling pathway.

  12. Search for new prototypes for the chemotherapy of filariasis: a chemotherapeutic and biochemical approach.

    Science.gov (United States)

    Bajpai, Preeti; Verma, Shailendra Kumar; Katiyar, Diksha; Tewari, Neetu; Tripathi, R P; Bansal, Iti; Saxena, J K; Misra-Bhattacharya, Shailja

    2005-04-01

    The antifilarial activity of two coumarin derivatives (A, B) and three glycosyl amine derivatives (D, E, F) was evaluated against a subperiodic strain of human lymphatic filarial parasite Brugia malayi by the intraperitoneal route at 50 mg/kg for 5 consecutive days. Of these, the two sugar derivatives (D and E) were selected for evaluation by the oral route based on their microfilaricidal (mild), macrofilaricidal and female worm sterilization efficacy using the i.p. route of administration. Compound E was finally selected for combination therapy on the basis of its microfilaricidal and embryostatic action by the oral route and its spectrum of activity against micro- and macrofilariae including embryostatic activity by the i.p. route. In addition, E also significantly inhibited the parasite DNA topoisomerase II. Compound A, in contrast, led to an enhanced adult worm burden. Compound B was toxic by the i.p. route, killing all of the treated animals before completion of the experiment. Some of these compounds demonstrated significant antifilarial efficacy of varying degree when tested in vitro Compounds B, D and F also killed adult B. malayi in vitro at 100 muM while 50 muM resulted in very slow motility of worms. Compound E in combination with a promising macrofilaricidal benzopyran derivative reported by us recently (compound C) did not show any synergistic or additive effect. These two compounds (C and E) individually on oral administration with either DEC or ivermectin significantly improved microfilaricidal efficacy in terms of intensity and duration of suppressed microfilaraemia. The combination of DEC with compound E demonstrated marginal enhancement in adulticidal efficacy, however, the embryostatic effect of the duo was significantly higher than that exerted by the individual agents. It may thus be inferred that in the absence of an adulticidal antifilarial drug, the use of potential antifilarials in combination with the standard filaricides may yield better

  13. A double-blind study of SB-220453 (Tonerbasat) in the glyceryltrinitrate (GTN) model of migraine.

    Science.gov (United States)

    Tvedskov, J F; Iversen, H K; Olesen, J

    2004-10-01

    The need for experimental migraine models increases as therapeutic options widen. In the present study, we investigated SB-220453 for efficacy in the glyceryltrinitrate (GTN) human experimental migraine model. SB-220453 is a novel benzopyran compound, which in animal models inhibits neurogenic inflammation, blocks propagation of spreading depression and inhibits trigeminal nerve ganglion stimulation-induced carotid vasodilatation. We included 15 patients with migraine without aura in a randomized double-blind crossover study. SB-220453 40 mg or placebo was followed by a 20-min GTN infusion. Headache, scored 0-10, was registered for 12 h, and fulfillment of International Headache Society (IHS) criteria was recorded until 24 h. Four subjects had a hypotensive episode after SB-220453 plus GTN but none after GTN alone. The reaction was unexpected, since animal models and previous human studies had shown no vascular or sympaticolytic activity with SB-220453. The study was terminated prematurely due to this interaction. GTN was consistent in producing headache and migraine that resembled the patients' usual spontaneous migraine. Nine patients had GTN on both study days. Peak headache score showed a trend towards reduction after SB-220453 compared with placebo (median 4 vs. 7, P = 0.15). However, no reduction was seen in the number of subjects experiencing delayed headache (8 vs. 8), number of subjects reporting migraine (6 vs. 8), migraine attacks fulfilling IHS criteria 1.1 or 1.7 (6 vs. 7) or IHS 1.1 alone (4 vs. 5). SB-220453 had no significant pre-emptive anti-migraine activity compared with placebo in this human model of migraine. Interaction between SB-220453 and GTN was discovered. This is important for the future development of the compound and underlines the usefulness of experimental migraine models.

  14. Brazilian Propolis: Correlation between Chemical Composition and Antimicrobial Activity

    Directory of Open Access Journals (Sweden)

    Kelly Salomão

    2008-01-01

    Full Text Available The chemical composition of ethanol extracts from samples of Brazilian propolis (EEPs determined by HPLC and their activity against Trypanosoma cruzi, Staphylococcus aureus, Streptococcus pneumoniae, Klebisiella pneumoniae, Candida albicans, Sporothrix schenckii and Paracoccidioides brasiliensis were determined. Based on the predominant botanical origin in the region of samples' collection, the 10 extracts were separated into three groups: A (B. dracunculifolia + Auraucaria spp, B (B. dracunculifolia and C (Araucaria spp. Analysis by the multiple regression of all the extracts together showed a positive correlation, higher concentrations leading to higher biological effect, of S. aureus with p-coumaric acid (PCUM and 3-(4-hydroxy-3-(oxo-butenyl-phenylacrylic acid (DHCA1 and of trypomastigotes of T. cruzi with 3,5-diprenyl-4-hydroxycinnamic acid derivative 4 (DHCA4 and 2,2-dimethyl-6-carboxyethenyl-2H-1-benzopyran (DCBEN. When the same approach was employed for each group, due to the small number of observations, the statistical test gave unreliable results. However, an overall analysis revealed for group A an association of S. aureus with caffeic acid (CAF and dicaffeoylquinic acid 3 (CAFQ3, of S. pneumoniae with CAFQ3 and monocaffeoylquinic acid 2 (CAFQ2 and of T. cruzi also with CAFQ3. For group B, a higher activity against S. pneumoniae was associated DCBEN and for T. cruzi with CAF. For group C no association was observed between the anitmicrobial effect and any component of the extracts. The present study reinforces the relevance of PCUM and derivatives, especially prenylated ones and also of caffeolyquinic acids, on the biological activity of Brazilian propolis.

  15. Dose-response-a challenge for allelopathy?

    Science.gov (United States)

    Belz, Regina G; Hurle, Karl; Duke, Stephen O

    2005-04-01

    The response of an organism to a chemical depends, among other things, on the dose. Nonlinear dose-response relationships occur across a broad range of research fields, and are a well established tool to describe the basic mechanisms of phytotoxicity. The responses of plants to allelochemicals as biosynthesized phytotoxins, relate as well to nonlinearity and, thus, allelopathic effects can be adequately quantified by nonlinear mathematical modeling. The current paper applies the concept of nonlinearity to assorted aspects of allelopathy within several bioassays and reveals their analysis by nonlinear regression models. Procedures for a valid comparison of effective doses between different allelopathic interactions are presented for both, inhibitory and stimulatory effects. The dose-response applications measure and compare the responses produced by pure allelochemicals [scopoletin (7-hydroxy-6-methoxy-2H-1-benzopyran-2-one); DIBOA (2,4-dihydroxy-2H-1,4-benzoxaxin-3(4H)-one); BOA (benzoxazolin-2(3H)-one); MBOA (6-methoxy-benzoxazolin-2(3H)-one)], involved in allelopathy of grain crops, to demonstrate how some general principles of dose responses also relate to allelopathy. Hereupon, dose-response applications with living donor plants demonstrate the validity of these principles for density-dependent phytotoxicity of allelochemicals produced and released by living plants (Avena sativa L., Secale cereale L., Triticum L. spp.), and reveal the use of such experiments for initial considerations about basic principles of allelopathy. Results confirm that nonlinearity applies to allelopathy, and the study of allelopathic effects in dose-response experiments allows for new and challenging insights into allelopathic interactions.

  16. Photoisomerization among ring-open merocyanines. I. Reaction dynamics and wave-packet oscillations induced by tunable femtosecond pulses

    Science.gov (United States)

    Ruetzel, Stefan; Diekmann, Meike; Nuernberger, Patrick; Walter, Christof; Engels, Bernd; Brixner, Tobias

    2014-06-01

    Upon ultraviolet excitation, photochromic spiropyran compounds can be converted by a ring-opening reaction into merocyanine molecules, which in turn can form several isomers differing by cis and trans configurations in the methine bridge. Whereas the spiropyran-merocyanine conversion reaction of the nitro-substituted indolinobenzopyran 6-nitro-1',3',3'-trimethylspiro[2H-1-benzopyran-2,2'-indoline] (6-nitro BIPS) has been studied extensively in theory and experiments, little is known about photoisomerization among the merocyanine isomers. In this article, we employ femtosecond transient absorption spectroscopy with variable excitation wavelengths to investigate the excited-state dynamics of the merocyanine in acetonitrile at room temperature, where exclusively the trans-trans-cis (TTC) and trans-trans-trans (TTT) isomers contribute. No photochemical ring-closure pathways exist for the two isomers. Instead, we found that (18±4)% of excited TTC isomers undergo an ultrafast excited-state cis→trans photoisomerization to TTT within 200 fs, while the excited-state lifetime of TTC molecules that do not isomerize is 35 ps. No photoisomerization was detected for the TTT isomer, which relaxes to the ground state with a lifetime of roughly 160 ps. Moreover, signal oscillations at 170 cm-1 and 360 cm-1 were observed, which can be ascribed to excited-state wave-packet dynamics occurring in the course of the TTC→TTT isomerization. The results of high-level time-dependent density functional theory in conjunction with polarizable continuum models are presented in the subsequent article [C. Walter, S. Ruetzel, M. Diekmann, P. Nuernberger, T. Brixner, and B. Engels, J. Chem. Phys. 140, 224311 (2014)].

  17. Effects of 3-styrylchromones on metabolic profiles and cell death in oral squamous cell carcinoma cells

    Directory of Open Access Journals (Sweden)

    Hiroshi Sakagami

    2015-01-01

    Full Text Available 4H-1-benzopyran-4-ones (chromones are important naturally-distributing compounds. As compared with flavones, isoflavones and 2-styrylchromones, there are only few papers of 3-styrylchromones that have been published. We have previously reported that among fifteen 3-styrylchromone derivatives, three new synthetic compounds that have OCH3 group at the C-6 position of chromone ring, (E-3-(4-hydroxystyryl-6-methoxy-4H-chromen-4-one (compound 11, (E-6-methoxy-3-(4-methoxystyryl-4H-chromen-4-one (compound 4, (E-6-methoxy-3-(3,4,5-trimethoxystyryl-4H-chromen-4-one (compound 6 showed much higher cytotoxicities against four epithelial human oral squamous cell carcinoma (OSCC lines than human normal oral mesenchymal cells. In order to further confirm the tumor specificities of these compounds, we compared their cytotoxicities against both human epithelial malignant and non-malignant cells, and then investigated their effects on fine cell structures and metabolic profiles and cell death in human OSCC cell line HSC-2. Cytotoxicities of compounds 4, 6, 11 were assayed with MTT method. Fine cell structures were observed under transmission electron microscope. Cellular metabolites were extracted with methanol and subjected to CE-TOFMS analysis. Compounds 4, 6, 11 showed much weaker cytotoxicity against human oral keratinocyte and primary human gingival epithelial cells, as compared with HSC-2, confirming their tumor-specificity, whereas doxorubicin and 5-FU were highly cytotoxic to these normal epithelial cells, giving unexpectedly lower tumor-specificity. The most cytotoxic compound 11, induced the mitochondrial vacuolization, autophagy suppression followed by apoptosis induction, and changes in the metabolites involved in amino acid and glycerophospholipid metabolisms. Chemical modification of lead compound 11 may be a potential choice for designing new type of anticancer drugs.

  18. The modulation of vascular ATP-sensitive K+ channel function via the phosphatidylinositol 3-kinase-Akt pathway activated by phenylephrine.

    Science.gov (United States)

    Haba, Masanori; Hatakeyama, Noboru; Kinoshita, Hiroyuki; Teramae, Hiroki; Azma, Toshiharu; Hatano, Yoshio; Matsuda, Naoyuki

    2010-08-01

    The present study examined the modulator role of the phosphatidylinositol 3-kinase (PI3K)-Akt pathway activated by the alpha-1 adrenoceptor agonist phenylephrine in ATP-sensitive K(+) channel function in intact vascular smooth muscle. We evaluated the ATP-sensitive K(+) channel function and the activity of the PI3K-Akt pathway in the rat thoracic aorta without endothelium. The PI3K inhibitor 2-(4-morpholinyl)-8-phenyl-1(4H)-benzopyran-4-one hydrochloride (LY294002) (10(-5) M) augmented relaxation in response to the ATP-sensitive K(+) channel opener levcromakalim (10(-8) to 3 x 10(-6) M) in aortic rings contracted with phenylephrine (3 x 10(-7) M) but not with 9,11-dideoxy-11alpha,9alpha-epoxy-methanoprostaglandin F(2alpha) (U46619; 3 x 10(-8) M), although those agents induced similar contraction. ATP-sensitive K(+) channel currents induced by levcromakalim (10(-6) M) in the presence of phenylephrine (3 x 10(-7) M) were enhanced by the nonselective alpha-adrenoceptor antagonist phentolamine (10(-7) M) and LY294002 (10(-5) M). Levels of the regulatory subunits of PI3K p85-alpha and p55-gamma increased in the membrane fraction from aortas without endothelium treated with phenylephrine (3 x 10(-7) M) but not with U46619 (3 x 10(-8) M). Phenylephrine simultaneously augmented Akt phosphorylation at Ser473 and Thr308. Therefore, activation of the PI3K-Akt pathway seems to play a role in the impairment of ATP-sensitive K(+) channel function in vascular smooth muscle exposed to alpha-1 adrenergic stimuli.

  19. Synthetic genistein derivatives as modulators of glycosaminoglycan storage

    Directory of Open Access Journals (Sweden)

    Kloska Anna

    2012-07-01

    Full Text Available Abstract Background Mucopolysaccharidoses (MPS are severe metabolic disorders caused by accumulation of undegraded glycosaminoglycans (GAGs in lysosomes due to defects in certain lysosomal hydrolases. Substrate reduction therapy (SRT has been proposed as one of potential treatment procedures of MPS. Importantly, small molecules used in such a therapy might potentially cross the blood–brain barrier (BBB and improve neurological status of patients, as reported for a natural isoflavone, 5, 7-dihydroxy-3- (4-hydroxyphenyl-4 H-1-benzopyran-4-one, also known as genistein. Although genistein is able to cross BBB to some extent, its delivery to the central nervous system is still relatively poor (below 10% efficiency. Thus, we aimed to develop a set of synthetically modified genistein molecules and characterize physicochemical as well as biological properties of these compounds. Methods Following parameters were determined for the tested synthetic derivatives of genistein: cytotoxicity, effects on cell proliferation, kinetics of GAG synthesis, effects on epidermal growth factor (EGF receptor’s tyrosine kinase activity, effects on lysosomal storage, potential ability to cross BBB. Results We observed that some synthetic derivatives inhibited GAG synthesis similarly to, or more efficiently than, genistein and were able to reduce lysosomal storage in MPS III fibroblasts. The tested compounds were generally of low cytotoxicity and had minor effects on cell proliferation. Moreover, synthetic derivatives of genistein revealed higher lipophilicity (assessed in silico than the natural isoflavone. Conclusion Some compounds tested in this study might be promising candidates for further studies on therapeutic agents in MPS types with neurological symptoms.

  20. iSTREEM(®) : An approach for broad-scale in-stream exposure assessment of "down-the-drain" chemicals.

    Science.gov (United States)

    Kapo, Katherine E; DeLeo, Paul C; Vamshi, Raghu; Holmes, Christopher M; Ferrer, Darci; Dyer, Scott D; Wang, Xinhao; White-Hull, Charlotte

    2016-10-01

    The "in-stream exposure model" iSTREEM(®) , a Web-based model made freely available to the public by the American Cleaning Institute, provides a means to estimate concentrations of "down-the-drain" chemicals in effluent, receiving waters, and drinking water intakes across national and regional scales under mean annual and low-flow conditions. We provide an overview of the evolution and utility of the iSTREEM model as a screening-level risk assessment tool relevant for down-the-drain products. The spatial nature of the model, integrating point locations of facilities along a hydrologic network, provides a powerful framework to assess environmental exposure and risk in a spatial context. A case study compared national distributions of modeled concentrations of the fragrance 1,3,4,6,7,8-Hexahydro-4,6,6,7,8,8,-hexamethylcyclopenta-γ-2-benzopyran (HHCB) and the insect repellent N,N-Diethyl-m-toluamide (DEET) to available monitoring data at comparable flow conditions. The iSTREEM low-flow model results yielded a conservative distribution of values, whereas the mean-flow model results more closely resembled the concentration distribution of monitoring data. We demonstrate how model results can be used to construct a conservative estimation of the distribution of chemical concentrations for effluents and streams leading to the derivation of a predicted environmental concentration (PEC) using the high end of the concentration distribution (e.g., 90th percentile). Data requirements, assumptions, and applications of iSTREEM are discussed in the context of other down-the-drain modeling approaches to enhance understanding of comparative advantages and uncertainties for prospective users interested in exposure modeling for ecological risk assessment. Integr Environ Assess Manag 2016;12:782-792. © 2016 SETAC.

  1. D1-dopamine receptor is involved in the modulation of the respiratory rhythmical discharge activity in the medulla oblongata slice preparation of neonatal rats in vitro%多巴胺D1受体参与新生鼠离体延髓脑片呼吸节律性放电的调节

    Institute of Scientific and Technical Information of China (English)

    焦勇钢; 吴中海

    2008-01-01

    本研究旨在探讨多巴胺D1受体在延髓离体脑片基本节律性呼吸放电调节中的作用.以改良的Kreb'S液(modified Kreb,s perfusion,MKS)恒温灌流Sprague-Dawley大鼠(0~3 d)离体延髓脑片标本,稳定记录到与之相连的舌卜神经根的呼吸节律性放电活动(respiratory rhythmical discharge activity,RRDA)后,第一组(n=5)先给予多巴胺Dl受体特异性激动剂[cis-(±)-1-(Aminomethyl)-3,4-dihydro-3-phenyl-1H-2-Benzopyran-5,6-Diolhy.drochlo-fide,A689301灌流10 min,用MKS洗脱后,再给予多巴胺D1受体特异性拮抗剂[R(+)-7-Chloro-8-hydroxy-3-methyl-1-pheny1-2,3,4,5-tetrahydro-1H-3-benzazepine hydrochloride,SCH-23390]灌流10 min;第二组(n=5)先给予A68930持续灌流10 min后再给予A68930+SCH-23390持续灌流10 min;观察各时间点舌下神经根RRDA的变化.结果显示,给予A68930灌流后呼吸周期(respiratory cycle,RC)和呼气时程(expiratory time,TE)显著缩短,放屯积分幅度(integral amplitude,IA)增加,吸气时程(inspiratory time,TI)无明显变化;给予SCH-23390后RC、TE显著延长、TI显著缩短、IA减小,而且A68930的作用可以被SCH一23390部分逆转.这些观察结果提示多巴胺D1受体参与了哺乳动物基本呼吸频率和幅度的调节.

  2. Evaluation of the angiogenesis inhibitor KR-31831 in SKOV-3 tumor-bearing mice using (64)Cu-DOTA-VEGF(121) and microPET.

    Science.gov (United States)

    Lee, Iljung; Yoon, Kwang Yup; Kang, Choong Mo; Lin, Xin; Chen, Xiaoyuan; Kim, Jung Young; Kim, Sung-Min; Ryu, Eun Kyoung; Choe, Yearn Seong

    2012-08-01

    KR-31831 ((2R,3R,4S)-6-amino-4-[N-(4-chloropheyl)-N-(1H-imidazol-2ylmethyl)amino]-3-hydroxyl-2-methyl-2-dimethoxymethyl-3,4-dihydro-2H-1-benzopyran), an angiogenesis inhibitor, was evaluated in tumor-bearing mice using molecular imaging technology. Pre-treatment microPET images were acquired on SKOV-3 cell-implanted nude mice after injection with (64)Cu-DOTA-VEGF(121). KR-31831 (50 mg/kg) was then injected intraperitoneally into the treatment group (n=3), while injection vehicle was injected into the control (n=4) and blocking (n=3) groups. After injections occurred daily for 28 days, all groups of mice underwent post-treatment microPET imaging after injection with (64)Cu-DOTA-VEGF(121). The post-treatment images showed high tumor uptake in the control group and reduced tumor uptake in both the blocking and treatment groups. ROI analysis of the tumor images revealed 6.25%±1.18% ID/g at 1 h, 6.55%±0.69% ID/g at 2 h, and 4.68%±0.63% ID/g at 16 h in the control group; 3.87%±0.45% ID/g at 1 h, 4.50%±0.44% ID/g at 2 h, and 3.63%±0.25% ID/g at 16 h in the blocking group; and 4.03%±0.74% ID/g at 1 h, 4.37%±0.67% ID/g at 2 h, and 3.83%±0.90% ID/g at 16 h in the treatment group. Biodistribution obtained after the post-treatment microPET imaging also demonstrated high tumor uptake (3.74%±0.27% ID/g) in the control group and reduced uptakes in both the blocking group (2.69%±0.73% ID/g, PKR-31831 is mediated through VEGFR2 and microPET serves as a useful molecular imaging tool for evaluation of a newly developed angiogenesis inhibitor, KR-31831.

  3. Development of new radiopharmaceuticals for imaging monoamine oxidase B

    Energy Technology Data Exchange (ETDEWEB)

    Vasdev, Neil, E-mail: neil.vasdev@utoronto.ca; Sadovski, Oleg; Moran, Matthew D.; Parkes, Jun; Meyer, Jeffrey H.; Houle, Sylvain; Wilson, Alan A.

    2011-10-15

    Introduction: Imaging monoamine oxidase B (MAO-B) in the central nervous system with PET is an important goal for psychiatric studies. We here report an improved and automated radiosynthesis of N-(6-[{sup 18}F]-fluorohexyl)-N-methylpropargylamine ([{sup 18}F]FHMP; [{sup 18}F]-1), as well as the radiosynthesis of two new promising candidates for imaging cerebral MAO-B, namely, carbon-11-labeled 3-(4-[{sup 11}C]-methoxyphenyl)-6-methyl-2H-1-benzopyran-2-one ([{sup 11}C]-2) and N-((1H-pyrrol-2-yl)methyl)-N-[{sup 11}C]-methyl-1-phenylmethanamine ([{sup 11}C]-3). Methods: Fluorine-18-labeled 1 was prepared via a tosyloxy precursor in 29%{+-}5% uncorrected radiochemical yield, relative to [{sup 18}F]-fluoride. Both carbon-11-labeled compounds were prepared with [{sup 11}C]CH{sub 3}I using the 'LOOP' method in 11% and 18% uncorrected radiochemical yields, respectively, relative to starting [{sup 11}C]CO{sub 2}. All radiotracers had specific activities >37 GBq/{mu}mol and were >98% radiochemically pure at end of synthesis (<40 min). All radiotracers were evaluated by ex vivo biodistribution studies in conscious rodents. Results: A major radioactive metabolite in the rodent brain was observed following administration of [{sup 18}F]-1. While [{sup 11}C]-2 had moderate brain penetration and good clearance from normal brain tissue, distribution of radioactivity in brain was indicative of free and nonspecific binding. Good brain uptake was observed with [{sup 11}C]-3 (0.8%-1.4% injected dose per gram at 5 min postinjection), binding appeared to be reversible and distribution conformed with regional distribution of MAO-B in the rat brain. Preinjection of 3 or L-deprenyl showed a modest reduction (up to 25%) of brain activity. Conclusion: Carbon-11-labeled 3 was found to have the most favorable properties of the radiotracers evaluated; however, the signal-to-noise ratio was too low to warrant further in vivo imaging studies. Alternative radiotracers for imaging MAO

  4. Microbial enrichment of a novel growing substrate and its effect on plant growth.

    Science.gov (United States)

    Trifonova, R; Postma, J; Schilder, M T; van Elsas, J D

    2009-10-01

    The quality of torrefied grass fibers (TGF) as a new potting soil ingredient was tested in a greenhouse experiment. TGF was colonized with previously selected microorganisms. Four colonization treatments were compared: (1) no inoculants, (2) the fungus Coniochaeta ligniaria F/TGF15 alone, (3) the fungus followed by inoculation with two selected bacteria, and (4) the fungus with seven selected bacteria. Cultivation-based and DNA-based methods, i.e., PCR-DGGE and BOX-PCR, were applied to assess the bacterial and fungal communities established in the TGF. Although colonization was not performed under sterile conditions, all inoculated strains were recovered from TGF up to 26 days incubation. Stable fungal and bacterial populations of 10(8) and 10(9) CFU/g TGF, respectively, were reached. As a side effect of the torrefaction process that aimed at the chemical stabilization of grass fibers, potentially phytotoxic compounds were generated. These phytotoxic compounds were cold-extracted from the fibers and analyzed by gas chromatography mass spectrometry. Four of 15 target compounds that had previously been found in the extract of TGF were encountered, namely phenol, 2-methoxyphenol, benzopyran-2-one, and tetrahydro-5,6,7,7a-benzofuranone. The concentration of these compounds decreased significantly during incubation. The colonized TGF was mixed with peat (P) in a range of 100%:0%, 50%:50%, 20%:80%, and 0%:100% TGF/P (w/w), respectively, to assess suitability for plant growth. Germination of tomato seeds was assessed three times, i.e., with inoculated TGF that had been incubated for 12, 21, and 26 days. In these tests, 90-100% of the seeds germinated in 50%:50% and 20%:80% TGF/P, whereas on average only 50% of the seeds germinated in pure TGF. Germination was not improved by the microbial inoculants. However, plant fresh weight as well as leaf area of 28-day-old tomato plants were significantly increased in all treatments where C. ligniaria F/TGF15 was inoculated compared

  5. Effect of coumaphos on cholinesterase activity, hematology, and biochemical blood parameters of bovines in tropical regions of Mexico.

    Science.gov (United States)

    Pardío, Violeta T; Ibarra, Nelly De J; Waliszewski, Krzysztof N; López, Karla M

    2007-05-01

    To assess the effect of coumaphos [O-(3-chloro-4-methyl-2-oxo-2H-1-benzopyran-7-yl) O,O-diethyl phosphorothioate] exposure on physiological responses during bovine production, acetylcolinesterase (AChE) and butyrylcholinesterase (BuChE) activities were measured in whole blood, erythrocytes, and plasma of healthy male steers (Bos Taurus x Bos indicus) sprayed with coumaphos at a non-lethal dose of 1 mg kg(- 1) body weight per day once every 14 (in vivo group) or 21 days (southern and central groups). Coumaphos topically administered at 1 mg/kg body weight per day to cattle under normal management practices in tropical areas produced a significant inhibition in erythrocyte (RBC) AChE and BuAChE activities when compared to baseline levels. RBC-AChE activity for the in vivo group decreased 71.3% (P < 0.05) and BuChE activity 59.1% (P < 0.05); RBC-AChE activity decreased 55.1% (P < 0.05) (southern group) and 43.4% (P < 0.05) (central group). Compared to the control specimens, steers from in vivo, southern, and central groups after 150 days of exposure had lower (P < 0.05) leukocyte count, absolute lymphocyte, erythrocyte, and platelet counts. Decreases in RBC-AChE activities correlated with decreased lymphocyte (r = 1.000, p = 0.01), erythrocyte (r = 1.000, p = 0.003), and platelet counts (r = 0.841, p = 0.036). Significantly increased BUN levels (P < 0.05) correlated with the decrease in RBC-AChE activities (r = - 0.997, p = 0.047) and with the decrease in absolute red blood cell (r = - 0.883, p = 0.020) and lymphocyte (r = - 0.825, p = 0.043) counts; increased (P < 0.05) total plasma protein levels correlated with the decrease in RBC-AChE activities (r = -0.998, p = 0.043), absolute red blood cell (r = - 0.998, p = 0.040), lymphocyte (r = - 0.893, p = 0.017), and platelet (r = -0.855, p = 0.030) counts. The physiological responses correlated with the erythrocyte acetylcholinesterase inhibition could be considered as early indicators or warning responses of bovine

  6. Polyphenols from leaves of Olea europaea%油橄榄叶多酚类成分研究

    Institute of Scientific and Technical Information of China (English)

    王晓飞; 李辰; 郑媛媛; 邸多隆

    2011-01-01

    目的 研究油橄榄Olea europaea的多酚类成分.方法 利用硅胶柱色谱手段,结合波谱方法分离鉴定油橄榄叶中的多酚类化合物.结果 从油橄榄叶醋酸乙酯部位中分离得到15个多酚类化合物,分别鉴定为:酪醇(1)、羟基酪醇(2)、羟基酪醇乙酰酯(3)、1H-2-苯并吡喃-6,7-二醇(4)、3,4-二羟基苯甲酸(5)、3-羟基-4-甲氧基苯甲酸(6)、异鼠李素(7)、圣草酚(8)、花旗松素(9)、槲皮素(10)、木犀草素(11)、女贞苷(12)、橄榄苦苷(13)、芹菜素-7-O-葡萄糖苷(14)、木犀草素-7-O-葡萄糖苷(15).结论 化合物4~9为首次从该种植物中分离得到.%Objective To study the polyphenols from the leaves of Olea europaea.Methods The polyphenols from ethyl acetate fraction were separated and purified by silica chromatographic method and identified by spectroscopic analysis.Results In combination with the data from literatures, the structure of 15 polyphenols had been elucidated on the basis of various spectroscopic methods, including tyrosol (1), hydroxytyrosol (2), hydroxytyrosol acetate (3), 1H-2-benzopyran-6,7-diol (4), 3,4-dihydroxyl-benzoic acid (5), 3-methoxyl-4-hydroxyl benzoic acid (6), isorhamnetin (7), eriodictyol (8), taxifolin (9), quercetin (10), luteolin (11),ligstroside (12), oleuropein (13), apigenin-7-O-glucoside (14), and luteolin-7-O-glucoside (15).Conclusion Compounds 4-9 are isolated from this plant for the first time.

  7. Chemical constituents and biological activities of Pyrethrum cinerariifolium%白花除虫菊化学成分及其生物活性的研究

    Institute of Scientific and Technical Information of China (English)

    郑喜; 王芯; 万春平; 李国红

    2016-01-01

    该研究应用柱层析法、薄层层析法和波谱法对白花除虫菊(Pyrethrum cinerariifolium)全株甲醇提取物进行分离纯化、结构鉴定,并分别采用 MTT 法、生物活性测定法和抑菌圈法测定所得化合物抗肿瘤、杀线虫和抑菌活性。结果表明:经波谱法鉴定化合物结构分别为 tulirinol (1),sesamin (2),β-cyclopyrethrosin (3)和3,5-dihydroxy-2-(4-hydroxy-3-methoxyphenyl)-7,8-dimethoxy-4H-1-benzopyran-4-one (4)。抗肿瘤活性显示化合物3对白血病细胞株 HL-60、肝癌细胞株 SMMC-7721、肺癌细胞株 A-549、乳腺癌细胞株 MCF-7和结肠癌细胞株SW480均表现出显著的抑制活性,其 IC50分别为3.800、2.890、2.930、4.600和5.160μmol•L-1;化合物1活性比3稍弱,其 IC50分别为5.020、10.760、12.310、12.310和12.250μmol•L-1;其中化合物3对各肿瘤细胞株 IC50值均小于顺铂。抗菌活性表明化合物3对大肠杆菌、蜡样芽孢杆菌、枯草芽孢杆菌和金黄色葡萄球菌均表现出明显的抑菌活性,且随着浓度增加活性逐渐增强,而化合物1和化合物2仅对部分菌株表现出微弱抑菌活性;杀线虫活性显示化合物3对秀丽隐杆线虫和全齿复活线虫均表现出显著的毒杀活性,且随着时间、浓度增加活性逐渐增强;而在同一时间点对秀丽隐杆线虫的毒杀活性强于全齿复活线虫。从白花除虫菊中分离所得4个化合物均为首次从该植株中发现,且首次报道了化合物3杀线虫和抑菌活性,值得进一步开发应用。%The chemical constituents of Pyrethrum cinerariifolium and the measure of antitumor activity, antibacterial ac-tivity and nematicidal activity of the compounds which were isolated from P. cinerariifolium were investigated. The whole plant organ of P. cinerariifolium was extracted with methanol three times, and the methanol extract of P. cinerariifolium was isolated by column chromatography and thin layer chromatography (TLC). The

  8. Study on Chemical Constituents of Volatile Oils in Drug Pair of Frankincense and Myrrh by GC-MS%乳香、没药药对配伍挥发油成分的GC-MS分析

    Institute of Scientific and Technical Information of China (English)

    王艳艳; 王团结; 宿树兰; 段金廒; 付小环

    2011-01-01

    Objective To discuss the influence ot drug compatibility on the components ot the volatile oil in Frankincense and Myrrh. Methods GC-MS system was used to analyze the volatile oil in Frankincense,Myrrh and their combination. The effect of compatibility on the components and content of volatile oil was evaluated. Results The results of GC-MS showed that cadina-1 ( 10 ) ,4-diene, yl-2H-1-Benzopyran, 3,5,6,8 a-tetrahydro-2,5,5,8 a-tetramethyl-, trans- was detectable in Frankincense but was undetectable in the drag pair; Phenol,2-(1,2-dimethyl-2- cyclopenten-l-yl)-, acetate, Naphthalene, 1,2,3,4-tetrahydro-l,6-dimethyl-4 -(1-methylethyl)-,(1 S-cis)-, Azulen-2-ol, 1,4-dimethyl-7-(l-methylethyl)- was detactable in Myrrh but was undectable in the drug pair;1-ethenyl -1-methyl - 2,4-diisopropyl -cyclohexane, 1,2,3,4- tetrahydro-1-methyl -8-Isopropylnaphthalene was dectable in the drug pair but was undetectable in the two single drugs. Conclusion The compatibility of the drug pair has an effect on components and contents in the volatile oil of Frankincense and Myrrh.%目的 考察乳香、没药药对配伍对挥发油化学成分的影响.方法 采用气-质联用系统对乳香、没药药材以及药对配伍中的挥发油进行分析,评价配伍对挥发油成分及含量的影响.结果 采用GC-MS对乳香、没药药材和药对挥发油的成分进行分析比较,发现乳香挥发油中检出的trans-3,5,6,8 a-四氢-2,5,5,8a-四甲基-y1-2H-1-苯并吡喃、荜澄茄-1(10),4-二烯等成分和没药挥发油中检出的2-(1,2-二甲基-2-环戊烯-1-y1)乙酸苯酯、(1S-cis)-1,2,3,4-四氢-1,6-二甲基-4-(1-甲基乙基)-萘、1,4-二甲基-7-(1-甲基乙基)-Azulen-2-醇、(-)-匙叶桉油烯醇等成分在药对挥发油中未检出,而相对于单煎所得的挥发油,药对挥发油中新增成分有1-乙烯基-1-甲基-2,4-二异丙基-环己烷、1,2,3,4-四氢-1-甲基-8-异丙基萘等.结论 乳香、没药药对配伍对所提挥发油的成分和含量都有较大的影响.

  9. Alkylphenols, Other Endocrine-Active Chemicals, and Fish Responses in Three Streams in Minnesota - Study Design and Data, February-September 2007

    Science.gov (United States)

    Lee, Kathy E.; Schoenfuss, Heiko L.; Jahns, Nathan D.; Brown, Greg K.; Barber, Larry B.

    2008-01-01

    included the presence and concentration of vitellogenin in plasma, gonadosomatic indices, and histological characterizations of liver and testes tissue. Hydrologic, chemical and biological characteristics were different among sites. The percentage of streamflow contributed by WWTP effluent (ranging from less than 1 to 79 percent) was greatest at the South Fork Crow River and least at the Grindstone River. WWTP effluent generally contributed the greatest percentage of streamflow during winter and late summer when streamflows were low. A wide variety of chemicals were detected. More chemicals were detected in WWTP effluent samples than in stream samples during most time periods. The most commonly detected chemicals in samples collected monthly and analyzed at the USGS National Research Program Laboratory were 2,6-di-tert-butyl-1,4-benzoquinone, 2,6-di-tert-butyl-4-methylphenol, 3-beta-coprostanol, 4-methylphenol, 4-nonylphenol (NP), 4-tert-octylphenol, bisphenol A, cholesterol, ethylenediaminetetraacetic acid, and triclosan. The chemicals 4-nonylphenolmonoethoxycarboxylate (NP1EC), 4-nonylphenoldiethoxycarboxylate (NP2EC), and 4-nonylphenoltriethoxycarboxylate (NP3EC) also were detected. Excluding nondetections, the sum of NP1EC through NP3EC concentrations ranged from 5.1 to 260 ug/L among all samples. NP was detected in upstream, effluent, and downstream samples in each stream during at least one time period. NP was detected in 49 percent of environmental samples. Excluding nondetections, concentrations of NP ranged from 100 to 880 nanograms per liter among all samples. NP was also detected in more than one-half of the bed-sediment samples. The most commonly detected wastewater indicator chemicals in samples analyzed by schedule 4433 at the USGS National Water Quality Laboratory were 3,4-dichlorophenyl isocyanate, acetyl-hexamethyl-tetrahydronaphthalene, benzophenone, cholesterol, hexahydrohexamethyl-cyclopenta-benzopyran, N,N-diethyl-meta-toluamide, and

  10. Mixed-lineage kinase inhibitors require the activation of Trk receptors to maintain long-term neuronal trophism and survival.

    Science.gov (United States)

    Wang, Leo H; Paden, Andrew J; Johnson, Eugene M

    2005-03-01

    Small-molecule mixed-lineage kinase (MLK) inhibitors, such as CEP-1347 [3,9-bis[(ethylthio)methyl]-(8R*,9S*,11S*)-(-)-9-hydroxy-9-methoxycarbonyl-8-methyl-2,3,9,10-tetrahydro-8,11-epoxy-1H,8H, 11H-2,7b,11a-triazadibenzo(a,g)cycloocta(cde)trinden-1-one] and CEP-11004 [3,9-bis-[(isopropylthio)methyl]-(8R*,9S*,11S*)-(-)-9-hydroxy-9-methoxycarbonyl-8-methyl-2,3,9,10-tetrahydro-8,11-epoxy-1H,8H,11H-2,7b,11a-triazadibenzo(a,g)cycloocta(cde)trinden-1-one], prevent c-Jun NH(2)-terminal kinase (JNK) pathway activation as well as the consequent neuronal cell death in many cell culture and animal models. In the cell culture model of nerve growth factor (NGF)-deprived sympathetic neurons, we find that CEP-11004 induced a approximately 3-fold increase in the mRNA and protein levels of TrkA, the NGF receptor. This resulted in ligand-independent activation of the TrkA receptor and the downstream phosphatidylinositol 3-kinase (PI3-kinase) pathway. Addition of the Trk inhibitor K252a [(8R*,9S*,11S*)-(-)-9-hydroxy-9-methoxycarbonyl-8-methyl-2,3,9,10-tetrahydro-8,11-epoxy-1H,8H,11H-2,7b,11a-triazadibenzo(a,g)cycloocta(cde)-trinden-1-one] or the PI3-kinase inhibitor LY294002 [2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one] significantly decreased the protein synthesis rates, mitochondrial function, and neuronal survival maintained by CEP-11004. In contrast to sympathetic neurons, MLK inhibitors maintain only short-term survival of potassium- and serum-deprived rat cerebellar granule neurons (CGNs), despite continuous inhibition of the JNK pathway. We found that similar to sympathetic neurons, CEP-11004 increased the levels of the Trk receptor expressed in CGNs, TrkB. However, CGNs required the addition of the exogenous ligand brain-derived neurotrophic factor (BDNF) to activate the PI3-kinase pathway and to maintain long-term survival. BDNF activated TrkB, but caused rapid down-regulation of activated receptors and maintained only minimal survival. Therefore, increase in TrkB levels

  11. Crystal Structure of 3S-hydroxy-7 Melleine%3S-7-羟基蜂蜜曲霉素分子的晶体结构

    Institute of Scientific and Technical Information of China (English)

    刘晓红; 许芳; 张奕; 刘丽红; 黄华容; 佘志刚; 林永成; 陈荣礼

    2006-01-01

    从红树林内生真菌Xylariasp.2508中分离到一个新的蜂蜜曲霉素型化合物3S-7-羟基蜂蜜曲霉素,这是首次从海洋真菌中获得该类结构的化合物并测定其晶体结构.通过核磁共振谱、红外光谱、质谱进行结构鉴定.其单晶经X射线衍射测试表明,该化合物晶体属单斜晶系,空间群为P21,化学式为C10H12O5,Mr=212.20.晶胞参数为:a=10.8884(19)(A),b=7.2284(13)(A),c=13.398(2)(A),β=104.217(3)°,V=1022.2(3)(A)3,Z=4,Dc=1.379 mg/m3,F(000)=448,μ=0.112 mm-1.结构由直接法解出,用全矩阵最小二乘法修正,最终偏离因子R=0.0498,wR=0.101.该分子由一个苯并吡喃环组成.通过与文献中已知3R-7-羟基蜂蜜曲霉素的熔点及旋光度对照,确定其绝对构型为3S.其初步抑菌实验结果表明,3S-7-羟基蜂蜜曲霉素菌在每纸片200 μg的剂量下对金黄色葡萄球菌(Staphylococcus aureus)没有表现出抑菌活性.%A new compound, 3S-hydroxy-7 melleine was isolated from the endophytic fungus Xylariasp No.2508 from the mangrove tree on the South China Sea coast. It was the first time that this kind of compound was isolated from marine fungus. The structure was elucidated by NMR data, infrared spectrum (IR) and mass spectrometry (MS). In addition, its structure was determined by the single-crystal X-ray diffraction analysis. It crystallized in monoclinic, space group P21 with a=10.8884(19) (A), b=7.2284(13) (A), c=13.398(2) (A), β=104.217(3)°,C10H10O4·H2O, Mr=212.20, V=1022.2(3) (A)3, Z=4, Dc=1.379 mg/m3, F(000)=448, μ=0.112 mm-1, the final R=0.0498, wR=0.101 for 2407 observed reflections (I>2σ(I)). The molecular backbone of the compound includes a benzopyran ring. By comparing with the melting point and the optical rotation of the known 3R-hydroxy-7 melleine in literature, the absolute configuration of the compound was determined as 3S. It didn't exhibit antibacterial activity against Gram-positive bacterium Staphylococcus aureus at 200μg/disk in the

  12. Determinants of Exposure to Fragranced Product Chemical Mixtures in a Sample of Twins

    Directory of Open Access Journals (Sweden)

    Matthew O. Gribble

    2015-01-01

    Full Text Available Fragranced product chemical mixtures may be relevant for environmental health, but little is known about exposure. We analyzed results from an olfactory challenge with the synthetic musk fragrance 1,3,4,6,7,8-hexahydro-4,6,6,7,8,8-hexamethyl-cyclopento-γ-2-benzopyran (HHCB, and a questionnaire about attitudes toward chemical safety and use of fragranced products, in a sample of 140 white and 17 black twin pairs attending a festival in Ohio. Data for each product were analyzed using robust ordered logistic regressions with random intercepts for “twin pair” and “sharing address with twin”, and fixed effects for sex, age, education, and “ever being bothered by fragrances”. Due to the small number of black participants, models were restricted to white participants except when examining racial differences. Overall patterns of association were summarized across product-types through random-effects meta-analysis. Principal components analysis was used to summarize clustering of product use. The dominant axis of variability in fragranced product use was “more vs. less”, followed by a distinction between household cleaning products and personal care products. Overall, males used fragranced products less frequently than females (adjusted proportionate odds ratio 0.55, 95% confidence interval 0.33, 0.93. This disparity was driven by personal care products (0.42, 95% CI: 0.19, 0.96, rather than household cleaning products (0.79, 95% CI: 0.49, 1.25 and was particularly evident for body lotion (0.12, 95% CI: 0.05, 0.27. Overall usage differed by age (0.64, 95% CI: 0.43, 0.95 but only hand soap and shampoo products differed significantly. “Ever being bothered by fragrance” had no overall association (0.92, 95% CI: 0.65, 1.30 but was associated with laundry detergent use (0.46, 95% CI: 0.23, 0.93. Similarly, black vs. white differences on average were not significant (1.34, 95% CI: 0.55, 3.28 but there were apparent differences in use of

  13. Study on inhibition of lymphangiogenesis in gastric cancer by NM-3%血管生成抑制剂抗胃癌微淋巴管生成的实验研究

    Institute of Scientific and Technical Information of China (English)

    达炜; 朱金水; 陈维雄; 王龙; 孙群

    2010-01-01

    目的 通过建立未分化人胃腺癌SGC-7901原位移植裸鼠模型,应用2-(8-羟基-6-甲氧基-1-氧-1-氢-2-苯并吡喃-3-羟基)丙酸(NM-3)和卡铂的腹腔化学治疗,观察其对裸鼠原位移植人胃癌微淋巴管生成的影响.方法 建立人胃癌SGC-7901原位种植BALB/C裸鼠模型28只,随机分为4组,每组7只,分别腹腔内注射0.9%氯化钠溶液、NM-3(10 mg/kg)、卡铂(5 mg/kg)和NM-3+卡铂,2次/周.8周后处死裸鼠,采用定量免疫组化染色检测淋巴管内皮细胞透明质酸受体(LYVE)-1、肾小球足突细胞膜黏蛋白、同源异型盒转录因子(Prox)-1;计数微淋巴管密度(LMVD)值.结果 卡铂组、NM-3组及NM-3+卡铂组LYVE-1表达值均较0.9%氯化钠溶液组下降,但差异无统计学意义(P值均>0.05);NM-3组及NM-3+卡铂组肾小球足突细胞黏蛋白、Prox-1值较0.9%氯化钠溶液组及卡铂组均显著下降(P<0.05).NM-3组及NM-3+卡铂组LMVD值分别为4.72±0.50和4.78±0.38,较0.9%氯化钠溶液组及卡铂组均显著下降[7.35±0.55和6.98±0.35,P<0.05].结论 NM-3能抑制胃癌微淋巴管生成,抑制肿瘤的生长和转移.%Objective To evaluate the inhibitory effect of 2-(8-hydroxy-6-methoxy-1-oxo-1-H-2-benzopyran-3-Y1) propionic acid (NM-3) on lymphangiogenesis in gastric cancer using orthotopic implantated tumor models of BALB/C nude mice. Methods A BALB/C nude mouse model of transplanted in situ human gastric cancer was established. Twenty-eight nude mice were divided into four groups with 7 each: control group, NM-3 treated group, carboplatin (10 mg/kg) treated group,and NM-3 combiantion group injected with normal saline, 5 mg/kg of NM-3, 10 mg/kg of carboplatin or 5 mg/kg of NM-3, + 10 mg/kg carboplatin, respectively, twice a week for 8 weeks. At the end of the 8th week, all mice were sacrificed for detection of lymphatic microvessel density (LMVD),lymphatic vessel endothelial hyaluranic acid receptor 1 (LYVE-1), podoplanin and Prox-1

  14. Novel 99mTc labeled

    Institute of Scientific and Technical Information of China (English)

    FAN; Caiyun

    2006-01-01

    ,C.,Liu,Q.,Wang,R.,Investigation on the in vivo σ receptor locating property of 125I-4-(N-benzylpiperidin)-4-iodo-phenylsulfonamide,J.Isotopes (in Chinese),2004,17:198-203.[23]Maier,C.A.,Wünsch,B.,Novel spiropiperidines as highly potent and subtype selective sigma-receptor ligands,Part 1,J.Med.Chem.,2002,45:438-448.[24]Maier,C.A.,Wünsch,B.,Novel sigma receptor ligands,Part 2.SAR of spiro[[2]benzopyran-1,4′-piperidines] and spiro[[2]ben-zofuran-1,4′-piperidines] with carbon substituents in position 3,J.Med.Chem.,2002,45:4923-4930.[25]Ding,Y.S.,Fowler,J.S.,Dewey,S.L.et al.,Synthesis and PET studies of fluorine-18-BMY 14802:A potential antipsychotic drug,J.Nucl.Med.,1993,34:246-254.[26]Kiesewetter,D.O.,de Costa,B.,Synthesis of N1-3-[18F]fluoro-propyl-N4-2-([3,4-dichlorophenyl]ethyl)piperazine,a high affinity ligand for sigma receptor,J.Labelled Cpd.Radiopharm.,1993,33:639-643.[27]Van Waarde,A.,Buursma,A.R.,Hospers,G.A.P.Et al.,Tumor imaging with 2σ-receptor ligands,18F-FE-SA5845 and 11C-SA4503:A feasibility study,J.Nucl.Med.,2004,45:1939-1945.[28]Elsinga,P.H.,Kawamura,K.,Kobayashi,T.et al.,Synthesis and evaluation of [18F]fluoroethyl SA4503 and SA5845 as PET ligand for the sigma receptor,J.Labelled Cpd.Radiopharm.,2001,44(Suppl.1):S4-S6.[29]Kawamura,K.,Ishii,S.,Kobayashi,T.et al.,Synthesis and evaluation of 11C-labeled SA4503,SA5845,and their ethyl derivatives as PET sigma receptor ligands,J.Labelled Cpd.Radiopharm.,2001,44(Suppl.1):S233-S235.[30]Kawamura,K.,Ishiwata,K.,Tajima,H.et al.,Synthesis and in vivo evaluation of [11C]SA6298 as a PET sigma1 receptor ligand,Nucl.Med.Biol.,1999,26:915-922.[31]Kawamura,K.,Ishiwata,K.,Tajima,H.et al.,In vivo evaluation of [11C]SA4503 as a PET ligand for mapping CNS sigma1 receptor,Nucl.Med.Biol.,2000,27:255-261.[32]Kawamura,K.,Elsinga,P.H.,Kobayashi,T.et al.,Synthesis and evaluation of 11C-and 18F-labeled 1-[2-(4-alkoxy-3-methoxy-phenyl)ethyl]-4-(3-phenylpropyl)piperazines as sigma receptor ligands for positron emission tomography studies

  15. Chemistry%化学

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    O612 01050129 具有微孔/大孔双孔道体系沸石材料的合成=Synthesis of Silicate Materials with Micro/Macroporous Bimodal Pore Systems[刊, 中]/王亚军(复旦大学),唐颐…∥高等学校化学学报.&2000, 21(7).&1013~1015 国家自然科学基金(29873011)资助 O612 01050130 一种新型磷酸钛大单晶的溶剂热法合成与结构研究=Studies on Solvothermal Synthesis and Structure of a New Titanium Phosphate Large Single Crystal[刊, 中]/郭阳虹(吉林大学),施展…∥高等学校化学学报.&2000, 21(7).&1010~1012 国家自然科学基金(29571011)资助 O613 01050131 一种氟气源的制备及热分解=Preparation and Thermal Decomposition of a Kind of Fluorine Source[刊, 中]/刘文元(西北核技术研究所),李辉…∥应用化学.&2000, 17(3).&300~303 国家"八六三”计划资助课题 O613 01050132 铒在HBTMPTP-正庚烷/水溶液间的传质动力学=Mass Transfer of Er(Ⅲ) between HBTMPTP Dissolved in n-Heptane and HAc-NaAc[刊, 中]/乐善堂(中科院长春应用化学所),马根祥…∥高等学校化学学报.&2000, 21(6).&832~835 在(30±0.5)℃下,用层流恒界面池研究了铒在HBTMPTP-正庚烷-0.2mol/L(H,Na)Ac萃取体系中的传质动力学.测定了该体系的界面张力,考察了水相酸度、萃取剂浓度、氯离子浓度、温度和比界面对萃取速率的影响.图6参16 国家自然科学基金(29771028,29801004)资助 O613 01050133 岩石中硼的提取分离及同位素组成的测定=Extraction and Separation of Boron in Rock Samples and lts lsotopic Determination by Thermal lonization Mass Spectrometry[刊, 中]/王刚(中科院青海盐湖所),王蕴慧…∥岩矿测试.&2000, 19(3).&169~172 介绍了一种用Na2CO3+K2CO3混合熔剂对岩石样品进行分解,用硼特效树脂和阴、阳混合离子交换树脂相结合进行岩石样品中硼的纯化分离的方法.采用该方法从岩石样品中提取、分离出来的硼能满足硼同位素质谱法测定的需要,不产生硼同位素分馏,硼同位素测定精度大多优于0.05%.表3参7 国家自然科学基金(29775028)资助 O614 01050134 有机/无机纳米杂化材料负载钛催化剂及聚合研究:Ⅰ.乙烯聚合=Study of Titanium Catalysts Supported on he Organic/Inorganic Hybrid and Polymerization Thereby:Ⅰ.Polymerization of Ethylene[刊, 中]/朱宁(中科院长春应用化学所),唐涛…∥应用化学.&2000, 17(3).&233~237 以苯乙烯与马来酸酐无规共聚物为有机组分,以正硅酸乙酯为无机组分,利用sol-gel方法制得纳米杂化材料,以此为载体合成了杂化材料载体钛系催化剂.图3表3参13 国家自然科学基金(29774028)资助 O614 01050135 硫酸锌与L-α-缬氨酸固体配合物的合成与表征=Synthesis and Characterization of Zinc Sulfate Complexes with L-α-Valine[刊, 中]/张晓玉(西北大学),杨旭武…∥应用化学.&2000, 17(3).&243~247 用半微量相平衡方法研究了ZnSO4-Val-H2O体系在25℃时的溶度图及饱和溶液折光率曲线.体系中未形成新化合物.合成了配合物Zn(Val)SO4*H2O、Zn(Val)2*H2O,并进行了表征.图2表3参13 国家自然科学基金(29871023)资助 O614 01050136 微乳液法制备超细包裹型铁粉=Preparation of Ultrafine Fe Particles by Microemulsion Method[刊, 中]/张朝平(贵州大学),邓伟…∥应用化学.&2000, 17(3).&248~251 应用W/O型微乳液法制备了纳米量级包裹型超细铁粉.由XRD、SEM、TEM和IR谱测试表明:它属于表面活性剂包裹型超细微粒.图3参9 国家自然科学基金(19872021)资助 O614 01050137 以12-钨(钼)磷杂多酸为掺杂剂的导电聚N,N-二甲基苯胺的合成与导电性=Synthesis and Conductivity of Conducting Poly N,N-Dimethaniline Doped by 12-Tungsto(Molybdo) Phosphoric Acid Used as Doping Agent[刊, 中]/张贞文(湖北大学),柳士忠…∥湖北大学学报(自然科学版).&2000, 22(3).&270~273 以12-钨(钼)磷杂多酸为掺杂剂,合成了聚N,N-二甲基苯胺.并利用ICP、元素分析、循环伏安、红外光谱及室温固体电导率的测定等手段进行了表征.结果表明,掺杂的PDMAn/PM12的室温导电率约为12-13 S/cm.图3参6 国家自然科学基金(29971010)资助 O614 01050138 Co(phen)2TATP3+与DNA在旋转金盘金环电极上的相互作用研究=Electrochemical Studies on the Interactions of the Complex of Co(phen)2TATP3+ with DNA at Rotating Au-Au Electrode[刊, 中]/李红(中山大学),蒋雄…∥高等学校化学学报.&2000, 21(7).&995~998 在pH=7.2的Tris缓冲溶液中,利用旋转环盘电极法研究了金电极上Co(phen)2TATP3+与DNA的相互作用,并根据扩散控制和电化学控制下得到的各种参数,对它们作用的模式进行了讨论.图3表2参13 国家自然科学基金(29871036)资助 O614 01050139 双金属杂原子分子筛CrCoBEA的合成、波谱及催化性能研究=Studies on the Synthesis, Spectroscopy and Catalytic Properties of CrCoBEA Metallosilicate[刊, 中]/王亚军(南开大学),唐祥海…∥高等学校化学学报.&2000, 21(7).&999~1004 采用水热晶化法首次合成出BEA结构含铬、钴双金属杂原子分子筛CrCoBEA,对晶化时间、晶化温度、成胶配比等合成条件进行了优化研究,测试其物相、波谱性质和催化性能.图6表1参15 国家自然科学基金重点项目(29733070)资助 O614 01050140 Ru(bpy)2+3在MCM-48中的组装及其发光性质=The Assembly of Tris(Bipyridine)Ruthenium(Ⅱ) in MCM-48 and Photoluminescent Properties of Assembly Products[刊, 中]/房铭(吉林大学),张萍…∥高等学校化学学报.&2000, 21(7).&1016~1017 国家自然科学基金(29771013,59795006)资助 O621 01050141 2-烯丙基-1,1-偕二金属环已烷的合成研究=Synthesis of 2-Allyl-1,1-Gemdimetallic Cyclohexane[刊, 中]/宋国强(江苏石油化工学院),王钒…∥合成化学.&2000, 8(3).&216~219 在无水乙醚中1-锂代环己烯和烯丙基溴化镁于无水溴化锌作用下发生金属化克兰荪重排反应,生成2-烯丙基-1,1-偕二金属环己烷.通过对温度、溶剂量和投料顺序等重排反应主要影响因素的考察和优化,可使2-烯丙基-1,1-偕二金属环己烷的收率达到65%.图2表3 国家自然科学基金(2947043)资助 O621 01050142 沙丘芦苇特有一小分子化合物及其对叶绿体的逆境保护效应[刊, 中]/浦铜良(兰州大学),程佑发…∥科学通报.&2000, 45(12).&1308~1313 用柱层析手段从沙漠地区沙丘芦苇叶片中分离得到一种为该生态型所特有的小分子物质,其化学特征与已报道的逆境胁迫诱导累积的溶质均不同,具多氨基芳香族强极性特征.其自然丰度与月平均气温和月极端高温值为指标的生境高温程度呈显著相关性.并主要存在于光合细胞器叶绿体中.该物质对离体叶绿体在高温下电子传递链的功能具有保护作用.图5表2参15 国家自然科学基金(39870062)资助 O621 01050143 亚胺氧自由基与酚类的反应=The Reaction of Iminoxy Radical with Phenols[刊, 中]/胡家欣(南京大学),吉民…∥高等学校化学学报.&2000, 21(7).&1045~1047 研究了亚胺氧自由基与酚类的反应,一步合成了取代苯并C023唑、萘并及喹啉并C023唑类衍生物,同时探讨了反应机理.表2参8 国家自然科学基金(29375052)资助 O621 01050144 新型磺酰胺类含氮手性配体的合成、晶体结构及催化苯乙酮的不对称氢转移反应=Studies on the Synthesis of New Sulfonylamide Ligand, Crystal Structure and Asymmetric Hydrogen Transfer Reaction of Acetophenone[刊, 中]/董春娥(中科院成都有机化学所),张俊龙…∥高等学校化学学报.&2000, 21(7).&1070~1072 国家自然科学基金(29973042)资助 O624 01050145 P+7原子团簇及其特殊配位=P+7 Clusters and Their Specical Coordinations[刊, 中]/陈明旦(厦门大学),罗海彬…∥厦门大学学报(自然科学版).&2000, 39(3).&341~346 在激光产生的磷原子团簇正离子的质谱图中,P+7具有最强的谱峰.使用分子图形软件设计出24种P+7的同分异构体,并进行分子力学、PM3半经验量子化学和ADF密度泛函优化.从各异构体成键能量的比较中可得知,最稳定的P+7构型是在最稳定的P6的双键位置增加1个磷原子所生成的结构.图2表2参23 国家自然科学基金资助 O626 01050146 硫醚双酐聚酰胺酸的合成及其热稳定性=The Synthesis and Thermal Stability of Thioether Dianhydride Poly(Amic Acid)[刊, 中]/王江洪(中科院感光化学所),沈玉全∥高等学校化学学报.&2000, 21(6).&879~883 合成了硫醚二酐和4-硝基-4′-[N,N-二(2-氨乙基)氨基]偶氮苯(二胺单体)及对应的硫醚聚酰胺酸,并对其结构进行表征.目前,这些聚合物在高敏感非线性光学材料方面显示着广泛的应用前景.图3参14 国家自然科学基金(69637010)资助 O626 01050147 具有光致变色和发光性能的有机化合物的合成及其性能研究=Synthesis and Characteristics of Compounds Having Both Photochromic and Fluorescent Character[刊, 中]/庞美丽(南开大学),王永梅…∥高等学校化学学报.&2000, 21(6).&903~907 以吲哚啉螺苯并吡喃与香豆素衍生物为原料.用DCC缩合酯化法在温和条件下合成了8种新的具有光致变色和发光性能的化合物.所得化合物同时具有光致变色和发荧光的双重特性.图2参15 国家自然科学基金(29872015)资助 O626 01050148 1,2,4-三唑并[3,2-d][1,5]-苯并氧氮杂C024化合物的合成=Synthesis of 1,2,4-Triazolo[3,2-d][1,5] Benzoxazepine Compounds[刊, 中]/刘现军(复旦大学),刘毅…∥高等学校化学学报.&2000, 21(7).&1052~1055 苯并二氢吡喃-4-酮的芳腙衍生的偶氮基碳正离子与腈发生1,3-偶极环加成反应,加成产物通过[1,2]-迁移扩环重排反应,形成新颖的三环系杂环化合物1,2,4-三唑并[3,2-d][1,5]-苯并氧氮杂C024化合物.图1表3参9 国家自然科学基金(A29872007)资助 O626 01050149 四(4-N-吡啶基)卟啉衍生物的合成及表征=Synthesis and Characterization of Meso-Tetrakis(4-Pyridyl) Porphyrin Derivatives[刊, 中]/刘淑清(吉林大学),孙浩然…∥应用化学.&2000, 17(3).&260~263 利用吡啶氮烷基化合成了一系列具有空间效应的水溶性和油溶性的四(4-N-吡啶基)卟啉衍生物及其对应的金属钴卟啉.表5参8 国家自然科学基金(29733090,29803003)资助 O627 01050150 η6-苯乙烯二羰基(三苯基膦)铬配合物的合成与聚合=Synthsis and Polymerization of η6-Styrenedicarbonyl (Triphenylphos- phine) Chromium Complexes[刊, 中]/田晓慧(华东理工大学),林嘉平…∥功能高分子学报.&2000, 13(4).&419~422 国家教委留学回国人员科研启动基金资助 O627 01050151 Schiff碱单核及双核配合物拟酶催化性能的研究=Studies on the Biomimetic Catalytic Character of New Schiff Base Mono-and Di-Nuclear Complexes[刊, 中]/陈新斌(湖南大学),朱申杰…∥高等学校化学学报.&2000, 21(7).&1048~1051 国家自然科学基金(29472055)资助 O627 01050152 Cp2TiCl2/BuiMgBr/THF体系还原二芳基二硒醚=Reduction of Diphenyldiselides with Cp2TiCl2/BuiMgBr/THF[刊, 中]/许新华(南开大学),黄宪∥高等学校化学学报.&2000, 21(7).&1073~1074 国家自然科学基金(29672008)资助 O629 01050153 环肽合成方法的研究进展=Progress in the Study on Synthetic Method of Cyclopeptide[刊, 中]/唐艳春(北京大学),田桂玲…∥高等学校化学学报.&2000, 21(7).&1056~1063 介绍了纯环肽与杂环肽合成的方法、策略及其优缺点,列举了一些常用缩合试剂,探讨了反应溶液浓度、线型肽前体化合物的结构及构象等因素对环化反应的影响.参64 国家自然科学基金(29772001)资助 O63 01050154 N-异丙基丙烯酰胺/N-乙烯基吡咯烷酮水凝胶的研究=Synthesis and Characterization of Copolymer Hydrogels of Poly(N-Isopropylacrylamide-co-N-Vinyl-2-Pyrrolidone)[刊, 中]/刘郁杨(西北工业大学),范晓东…∥功能高分子学报.&2000, 13(4).&380~384 陕西省自然科学基金资助 O63 01050155 组织工程细胞支架及其细胞亲和性改进研究进展=Cells Scaffold for Tissue Engineering and Improvement of Cells Affinity between the Cells and Cells Scaffold[刊, 中]/杨健(中科院化学所),贝建中…∥功能高分子学报.&2000, 13(4).&455~460 国家"973计划” O63 01050156 新型的乙烯聚合催化剂=Novel Catalysts for Ethylene Polymerization[刊, 中]/罗祥(中山大学),伍青∥功能高分子学报.&2000, 13(4).&481~486 广东省自然科学基金资助 O631 01050157 衣康酸对聚丙烯腈原丝结构和性能的影响=Effect of Itaconic Acid on the Structure and Properties of PAN Precursor[刊, 中]/张旺玺(山东工业大学)∥高分子学报.&2000, (3).&287~291 控制单体配比,采用丙烯腈与衣康酸自由基溶液共聚,以偶氮二异丁腈为引发剂在溶剂二甲基亚砜中合成了聚丙烯腈原丝纺丝溶液,并纺制了碳纤维前驱体聚丙烯腈原丝.通过元素分析、IR、DSC、13C-NMR等手段,讨论了共聚单体衣康酸对共聚反应及聚丙烯腈原丝结构和性能的影响.图3表3参11 国家自然科学基金(59783002)资助 O631 01050158 Cr(Ⅱ)与过氧化莰烷酮酰体系引发的活性自由基聚合反应研究=Study on "Living” Radical Polymerization Initiated by Aged Chronum (Ⅱ) Acetate and Ketopinyl Peroxide[刊, 中]/李增昌(中国科学技术大学),吴承佩…∥高分子学报.&2000, (3).&330~334 合成了过氧化莰烷酮酰(KPO),用它和Cr(Ac)2组成的氧化还原体系经陈化后,引发甲基丙烯酸甲酯,甲基丙烯酸β-羟乙酯和丙烯酸进行了聚合反应.其中甲基丙烯酸甲酯具有活性聚合特征.并有效引发含羟基单体如甲基丙烯酸β羟乙酯和丙烯酸进行聚合.图2表4参10 国家自然科学基金(29774027)资助 O631 01050159 MgCl2负载(dbm)2Ti(OPh)2催化乙烯聚合=Ethylene Polymerization with MgCl2-Supported(dbm)2Ti(OPh)2[刊, 中]/阎卫东(中科院化学所),洪瀚…∥高分子学报.&2000, (3).&358~360 报道了用MgCl2负载的二苯氧基二(二苯甲酰甲烷)钛铬合物(dbm)2Ti(OPh),以甲基铝氧烷为助催化剂,常压催化乙烯聚合的结果.图1表2参8 国家自然科学基金(29734141)重点资助 O631 01050160 单分散聚苯乙烯乳胶有序膜在二氧化硅多孔材料制备中的模板作用=Template Effects of the Ordered Film Formed from Monodispersed Polystyrene Latex[刊, 中]/杨振忠(中科院化学所),齐凯…∥高分子学报.&2000, (3).&364~367 以单分散聚苯乙烯乳胶室温形成的有序膜为模板,采用快速溶胶凝胶方法,制备了聚苯乙烯/二氧化硅杂化物,高温烧蚀除去有机物得到了二氧化硅有序孔材料,并讨论了聚苯乙烯有序膜的模板作用机理.图5参15 国家自然科学基金(29774038)资助 O631 01050161 新型含环氧端基聚芳醚酮的合成及表征=Synthesis and Characterization of a Novel Epoxy-Terminated Poly(Aryl Ether Ketoe)[刊, 中]/宣英男(大连理工大学),蹇锡高…∥高分子学报.&2000, (4).&407~410 国家"九五”攻关项目 O631 01050162 超支化聚(胺-酯)的分子设计及其制备=Synthesis and Characterization of Hyperbranched Poly(Amine-Ester)[刊, 中]/陆玉(中国科学技术大学),林德…∥高分子学报.&2000, (4).&411~414 国家自然科学基金(59673026)资助 O631 01050163 聚苯基单醚喹C023啉薄膜的性能与物理老化=The Relations between Properties and Physical Aging of Polyphenylquinoxaline Film[刊, 中]/刘万军(中科院化学所),沈静姝…∥高分子学报.&2000,(4).&415~419 国家科委基础性研究重大项目 O631 01050164 端基附壁模型聚合物环形链的构象统计理论=Configurational Statistics of Model Polymer Loop Chains Attached to a Surface[刊, 中]/廖琦(四川大学),吴大诚∥高分子学报.&2000,(4).&420~425 国家自然科学基金(29774016)资助 O631 01050165 拉格朗日-欧拉方法模拟高分子复杂流体平面收缩流动=The Lagrangian-Eulerian Method:Solutions for Planar Contraction Flow[刊, 中]/李险峰(中科院化学所),袁学锋…∥高分子学报.&2000,(4).&432~437 国家自然科学基金(29634030)资助 O631 01050166 气体膜分离用过渡金属有机络合物-聚酰亚胺杂化材料的研究=Study on Transitional Metal Organic Complex-Polyimide Hybrid Material for Gas Separation Membranes[刊, 中]/史德青(石油大学),孔瑛…∥高分子学报.&2000,(4).&457~461 科技部国家重点基础研究发展规划项目 O631 01050167 原子转移自由基聚合制备聚(丙二醇-g-苯乙烯)=Synthesis of Poly(Propylene Glycol-g-Styrene) from Atom Transfer Radical Polymerization[刊, 中]/黄昌国(华东理工大学),万小龙…∥高分子学报.&2000,(4).&467~471 国家自然科学基金(29634010)资助 O631 01050168 聚苯胺嵌入氧化石墨复合物的合成及表征=Synthesis and Characterization of Polyaniline Intercalated Graphite Oxide Composite[刊, 中]/刘平桂(北京航空材料研究院),龚克成∥高分子学报.&2000,(4).&492~495 国家自然科学基金(59836230)资助 O631 01050169 乙交酯/丙交酯共聚物的体内外降解行为及生物相容性研究=Study on the Biocompatibility and Degradation Behavior of Poly(l-Lactide-co-Glycolide) in vitro and in vivo[刊, 中]/蔡晴(中科院化学所),贝建中…∥功能高分子学报.&2000, 13(3).&249~254 国家重点基础研究发展规划(973)资助 O631 01050170 动态下HTDB/TDI体系凝胶化的研究=Study on the Gelation of Hydroxy-Teminated Polybutadiene/Diisocyanate Blends under Dynamic Condition[刊, 中]/邱方正(华东理工大学),陈建定∥功能高分子学报.&2000, 13(3).&289~292 国家教育部博士点基金资助 O631 01050171 含偶氮苯生色团的聚醚的合成与热致液晶性质=Synthesis and Thermotropic Liquid Crystalline Properties of Two Polyethers Containing Azobenzene Groups[刊, 中]/梁旭霞(中山大学),张灵志…∥功能高分子学报.&2000, 13(3).&277~284 广东省自然科学基金资助 O631 01050172 CoSalphen在壳聚糖上的固定化及对DOPA的催化氧化研究=CoSalphen Immobilized onto Chitosan and Its Activate in Catalytic Oxidation of DOPA[刊, 中]/马会宣(陕西师范大学),胡道道…∥功能高分子学报.&2000, 13(3).&301~305 陕西省自然科学基金资助 O631 01050173 环氧基活化凝胶的开环反应=Ring-Opening Reaction on Epoxy-Activated Resin[刊, 中]/查娟(生物反应器工程国家重点实验室),刘坐镇…∥功能高分子学报.&2000, 13(3).&321~324 由自制的乙酸乙烯酯-甲基丙烯酸缩水甘油酯-甲基丙烯酸烯丙酯大孔共聚物与氨水反应,实现了环氧基的开环反应.考查了不同的氨水浓度、反应温度和时间对胺化转化率的影响,并测定了形成的伯胺基的pKa值为6.9.图4表1参12 国家自然科学基金(29706002)资助 O631 01050174 纳米自组装聚电解质超薄多层膜=Polyelectrolyte Ultrathin Multilayer Films by Nano-Self-Assembly Process[刊, 中]/孙启龙(华南理工大学),王朝阳∥功能高分子学报.&2000, 13(3).&332~336 国家杰出青年科学基金(29725411)与国家自然科学基金(29804003)资助 O631 01050175 十二烷基硫酸钠减慢二苯胺-4-重氮盐及重氮树脂在水溶液中热分解反应的机理=The Mechanism of Thermal Decomposition of DDS and DR in Aqueous Solution Delayed by SDS[刊, 中]/杨伯C025(北京大学),曹维孝∥高等学校化学学报.&2000, 21(6).&969~975 国家自然科学基金(59633110及29774001)资助 O631 01050176 芘标记磺酸基聚电解质在水与DMSO中的荧光光谱=Fluorescence Spectra of Pyrene Labeled Polyelectrolytes with Sulfonate Groups in Water and DMSO[刊, 中]/高峰(华南理工大学),严宇…∥高等学校化学学报.&2000, 21(6).&976~979 国家自然科学基金(29804003)与国家杰出青年科学基金(29725411)资助 O631 01050177 尼龙1012的Brill转变=Brill Transition of Nylon 1012[刊, 中]/李勇进(上海交通大学),颜德岳…∥高等学校化学学报.&2000, 21(6).&983~984 国家自然科学基金(29974017)资助 O631 01050178 热致液晶含氯侧基聚芳醚酮的单晶状条带织构=The Single Crystal-Like Banded Textures in the Films of a Thermotropic Liquid Crystalline Poly(Aryl Ether Ketone) Containing a Lateral Chloro Group[刊, 中]/富露祥(中科院长春应用化学所),张善举…∥高等学校化学学报.&2000, 21(6).&988~990 国家自然科学基金(29974032)资助 O631 01050179 具有RAFT链转移过程的活性自由基聚合的Monte Carlo模拟=Monte Carlo Simulation on Living Radical Polymerization with RAFT Process[刊, 中]/李莉(复旦大学),何军坡…∥高等学校化学学报.&2000, 21(7).&1146~1148 国家自然科学基金资助 O631 01050180 C60-苯乙烯-顺丁烯二酸酐的三元自由基共聚=Free Radical Ternary Copolymerization of C60 with Styrene and Maleic Anhydride[刊, 中]/官文超(华中理工大学),雷洪…∥高等学校化学学报.&2000, 21(7).&1149~1150 国家自然科学基金(29674010)资助 O631 01050181 聚(苯乙烯-b-2-甲基-2-C023唑啉)嵌段共聚物的合成=Studies on Synthesis of P(S-b-Me-OXZ) Block Copolymers[刊, 中]/刘燕飞(华东理工大学),万小龙…∥高等学校化学学报.&2000, 21(7).&1157~1159 国家自然科学重点基金(29634010-2)资助 O631 01050182 乙丙共聚物接枝马来酸酐反应机理的模拟研究=Investigation of Reaction Mechanism of Maleic Anhydride Grafted onto Ethylene-Propylene Copolymer[刊, 中]/黄宏亮(中科院长春应用化学所),姚占海…∥应用化学.&2000, 17(3).&256~259 国家自然科学基金(59873022)资助 O631 01050183 稀土催化丁二烯-异戊二烯共聚合过程中的原位环化反应=In situ Cyclization in the Course of Butadiene Isoprene Copolymerization with Nd-Based Catalyst[刊, 中]/董为民(中科院长春应用化学所),逄束芬…∥应用化学.&2000, 17(3).&272~275 国家自然科学基金(29574179)资助 O631 01050184 计算机模式识别在颜料表面改性中的应用=Study on the Application of Pattern Recognition with a Computer in Surface Modification of Pigment[刊, 中]/严乐美(天津大学),杨桂琴…∥天津大学学报.&2000, 33(3).&344~347 国家自然科学基金(29376254)资助 O631 01050185 共混物中聚氯乙烯凝胶化度测试=Studies on Gelling Value of Polyvingyl Chloride in the Blend[刊, 中]/何本桥(湖北大学),张玉红…∥湖北大学学报(自然科学版).&2000, 22(4).&367~370 湖北省自然科学基金资助 O631 01050186 二氧化硅模板技术制备三维有序聚苯乙烯孔材料[刊, 中]/容建华(中科院化学所),杨振忠…∥科学通报.&2000, 45(15).&1627~1630 以单分散二氧化硅颗粒形成的有序结构为模板,制备了聚苯乙烯孔材料.通过扫描电子显微镜观察了模板、复合物及有序多孔聚苯乙烯的形态.结果表明,所合成的多孔材料中,孔大小均匀,空间排布高度有序,且结构与模板中二氧化硅小球自组装方式完全相同.图6参8 国家自然科学基金(20023003)资助 O631 01050187 光导性偶氮聚合物材料的设计[刊, 中]/周雪琴(浙江大学),陈红征…∥自然科学进展.&2000, 10(6).&543~548 以电荷转移理论为指导,对偶氮聚合物材料结构进行设计,获得了两类光导性偶氮聚合物材料,并对其结构和光导性进行了分析与讨论.图4表1参16 国家自然科学基金(59333071,69890230)资助 O631 01050188 聚合物复合材料中填料的摩擦学作用=Tribological Actions of Filiers in Polymer Composites[刊, 中]/薛群基(中科院兰州化学物理所),阎逢元∥中国科学基金.&2000, 14(4).&211~215 综述了就填料对聚合物复合材料的结构和摩擦学行为的影响,并对目前研究过程中所存在的问题进行了简要分析.图3参29 国家自然科学基金资助 O633 01050189 双马来酰亚胺树脂链结构对熔点和反应活性的影响=The Effect of Chain Structure of Bismaleimide Resins on Melting Point and Reactivity of Reaction[刊, 中]/刘润山(湖北省化学研究所),赵三平…∥功能高分子学报.&2000, 13(4).&487~493 湖北省自然科学基金资助 O636 01050190 高分子固体电解质材料研究进展=Advances in Material of Solid Polymer Electrolytes[刊, 中]/赵地顺(河北科技大学),孙凤霞…∥功能高分子学报.&2000, 13(4).&469~475 河北省自然科学基金资助 O64 01050191 Ru(bpy)2(NCS)2染料敏化CdS/Zn2+-TiO2复合半导体纳米多孔膜的光电化学=Photoelectrochemistry of CdS/Zn2+-TiO2 Composite Semiconductor Nanoporous Films Sensitized by Ru-(bpy)2(NCS)2 Dye[刊, 中]/张莉(北京大学),王艳芹…∥高等学校化学学报.&2000, 21(7).&1075~1079 国家自然科学基金(29773003)资助 O641 01050192 富勒烯笼结构的平面展示法[刊, 中]/叶大年(中科院地质与地球物理所),艾德生…∥科学通报.&2000, 45(16).&1781~1785 采用船式F5和F6与海燕式F5和F6分别表示富勒烯笼几何结构的两种平面图.这些平面图最大的优点是:(1)不破坏富勒烯笼的空间对称性;(2)可以非常清楚地展示出结构中F5和F6的配位形式.这为富勒烯笼的结构几何研究打下了基础.图3参17 国家自然科学基金(49872019)资助 O641 01050193 布朗扩散系数对胶体分形粒子簇扩散控制聚集动力学影响的Monte Carlo模拟=Monte Carlo Simulation to the Effect of Cluster Brownian Diffusion Coefficient on the Colloidal Fractal Cluster Aggregation Kinetics[刊, 中]/王惠(南开大学),王贵昌…∥高等学校化学学报.&2000, 21(6).&922~925 使用表征粒子簇结构的几何形状因子,通过对扩散控制聚集过程的模拟,从微观或介观层次研究了粒子簇结构对粒子簇增长速率和速率常数的影响规律,并与实验结果进行了对比分析.图4表1参15 国家自然科学基金(29573109)资助 O641 01050194 一些富勒烯C60双加成物稳定性的理论研究=Theoretical Studies on the Stabilities of Some C60 Bis-Adducts[刊, 中]/陈中方(南开大学),王贵昌…∥高等学校化学学报.&2000, 21(7).&1127~1129 国家自然科学基金(29773022)资助 O641 01050195 M(bpy)+32(M=Fe,Ru,Os)电子结构与相关性质=Electronic Structure and Related Chemical Properties of Complexes(M=Fe,Ru,Os)[刊, 中]/郑康成(中山大学),匡代彬…∥物理化学学报.&2000, 16(7).&608~612 报道了对配合物(M=Fe,Ru,Os)的量子化学密度泛函法研究的结果,探讨的电子结构特征及相关性质,特别是中心原子对配合物的配位键长、光谱性质、电荷布居及化学稳定性等的影响规律.图2表6参14 国家自然科学基金资助 O642 01050196 纳米HZSM-5分子筛的热稳定性=Thermostability of Nanosized HZSM-5 Zeolite[刊, 英]/张维萍(中科院大连化物所),包信和∥催化学报.&2000, 21(3).&195~196 科技部攀登计划项目 O642 01050197 微量热法研究硒对大肠杆菌生长代谢的作用=Microcalorimetric Study of the Action of Seleniumcontaining Complexes on Growth Metabolism of Escherichia coli[刊, 中]/李曦(武汉大学),刘义…∥物理化学学报.&2000, 16(6).&568~572 采用微量热法测定了Na2SeO3、吗啉、硒代吗啉和N,N'-亚基双硒代吗啉对大肠杆菌作用的热功率输出曲线,根据热功率输出曲线求算在这些药物作用下,大肠杆菌生长代谢的速率常数,并对它们的作用特征进行了比较.图2表1参7 国家自然科学基金资助 O642 01050198 反相气相色谱法测定溶剂在PBMA中的无限稀释活度系数=Measurement of Infinite Diluted Activity Coefficient of Solvent in PBMA by Inverse Gas Chromatography Method[刊, 中]/冯媛媛(华东理工大学),叶汝强…∥华东理工大学学报.&2000, 26(3).&305~308 用反相气相色谱方法测定了16种溶剂在聚甲基丙烯酸丁酯(PBMA)中343~433K范围内的无限稀释活度系数,并用周浩等的高分子溶液分子热力学模型对实验结果进行了关联,结果令人满意.图8表2参15 国家自然科学基金(29976011)资助 O643 01050199 海泡石对非晶态NiB合金催化剂的改性研究=Studies on the Modification of Sepiolite for Amorphous NiB Alloy Catalyst[刊, 中]/石秋杰(南昌大学),陈昭萍…∥物理化学学报.&2000, 16(6).&501~506 以常压气相苯加氢为探针反应,考察了酸改性前后海泡石的加入对非晶态NiB合金催化剂的加氢及抗硫性能的影响,用TPR、TPD表征了催化剂的表面性质,测定了酸改性前后海泡石的比表面及孔径分布,并与活性进行了关联.图3表3参12 国家自然科学基金资助 O643 01050200 F+NCO反应的机理和动力学=Mechanism and Kinetics of the F+NCO Reaction[刊, 中]/侯华(山东大学),王宝山…∥物理化学学报.&2000, 16(6).&517~521 高等学校博士学科点专项科研基金资助 O643 01050201 激光溅射下原子团簇生长的非平衡力学(Ⅱ)=Non-Equilibrium Kinetics of Clusters Growth under Laser Ablation(Ⅱ)[刊, 中]/曹玉群(厦门大学),黄荣彬…∥物理化学学报.&2000, 16(7).&621~626 国家自然科学基金(29890210,29773040)资助 O643 01050202 双(2,4-二甲基戊二烯基)氯化钆的合成及晶体结构=Synthesis and Structure of Bis(2,4-Dimethylpentadienyl)Gadolinium Chloride[刊, 中]/王建辉(吉林大学),母瀛…∥高等学校化学学报.&2000, 21(6).&829~831 国家自然科学基金(29672013,29754143)资助 O643 01050203 温控相转移催化&&水/有机两相催化新进展=Thermoregulated Phase-Transfer Catalysis&&Recent Advances in Water/Organic Biphasic Catalysis[刊, 中]/金子林(大连理工大学),梅建庭…∥高等学校化学学报.&2000, 21(6).&941~946 综述了"温控相转移催化”的原理,温控膦配体的设计、合成及其在水溶性极小的底物高碳烯烃的水/有机两相氢甲酰化反应中的应用效果.图4表5参35 国家自然科学基金(29792074)资助 O643 01050204 Cr-HMS合成、表征及其在催化氧化反应中的应用=Synthesis, Characterization of Cr-HMS and the Application in Catalytic Oxidation[刊, 中]/乐洪咏(复旦大学),华伟明…∥高等学校化学学报.&2000, 21(7).&1101~1104 合成了一系列不同Cr含量的Cr-HMS介孔分子筛,并对其物化性质进行了表征.结果表明,Cr的引入降低了介孔分子筛骨架结构的规整度和孔径的均一程度.Cr-HMS对苯羟基化和环己烷氧化反应均表现出良好的催化活性.并且随Cr含量的增加,催化活性呈火山型分布.图3表3参14 国家自然科学基金(29873011)资助 O643 01050205 新型甲烷无氧芳构化催化剂MoO3/MCM-49=A Novel Catalyst of MoO3/MCM-49 over Nonoxidative Aromatization of CH4[刊, 中]/许宁(吉林大学),阚秋斌…∥高等学校化学学报.&2000, 21(7).&1113~1114 国家自然科学基金(29973011)资助 O643 01050206 以TiCl4为钛源合成钛硅分子筛=Synthesis of Titanosilicalite Using Titanium Tetrachloride as Titanium Source[刊, 英]/张义华(大连理工大学),王祥生∥催化学报.&2000, 21(3).&197~198 以四丙基溴化铵为模板剂,以四氯化钛为钛源,研究了合成小晶粒钛硅分子筛(TS-1)的合成路线.合成的TS-1样品在丙烯环氧化反应中显示有较高的活性(96%)和选择性(95%),H2O2利用率高于95%.图3表1参8 国家自然科学基金(29792071)资助 O643 01050207 稀土氧化物在Fe1-xO基氨合成催化剂中的作用规律=Regular Effect of Rare Earth Oxide on Fe1-xO Catalyst for Ammonia Synthesis[刊, 中]/季德春(浙江工业大学),李小年∥催化学报.&2000, 21(3).&199~203 考察了稀土氧化物对Fe1-xO基催化剂性能的影响.结果表明,稀土氧化物与Fe1-xO作用生成一种复合氧化物REFeO3,从而促进了催化剂的还原,这种促进作用随稀土离子半径不同呈现规律性变化.图1表6参14 国家自然科学基金(29706011)资助 O643 01050208 光电催化降解磺基水杨酸的研究=Study of Photoelectrocatalytic Degradation of Sulfosalicylic Acid[刊, 中]/刘鸿(中科院大连化学物理所),冷文华∥催化学报, 21(3).&209~212 建立了以TiO2/Ni为工作电极、泡沫镍为对电极、饱和甘汞电极为参比电极的光电催化反应体系,研究了在磺基水杨酸(SSal)的光电催化降解过程中,外加电压和溶液pH值对降解速率的影响.图5表1参18 国家自然科学基金(29877024)资助 O643 01050209 多孔TiO2薄膜的表面微结构对甲基橙光催化脱色的影响=Effect of Surface Microstructure of Porous TiO2 Thin Films on Photocatalytic Decolorization of Methyl Orange[刊, 中]/余家国(武汉工业大学),赵修建∥催化学报.&2000, 21(3).&213~216 湖北省自然科学基金资助 O643 01050210 γ-Mo2N催化剂上的乙炔选择加氢=Selective Hydrogenation of Ethyne on γ-Mo2N[刊, 中]/郝志显(中科院大连化学物理所),魏昭彬∥催化学报.&2000, 21(3).&217~220 对γ-Mo2N催化剂上乙炔加氢反应进行了研究.在150℃的反应温度下,乙炔转化率为95%,乙烯选择性达80%,乙烷选择性为4%,丁烯选择性为10%.反应温度和空速对产物的选择性没有明显的影响.图4参17 国家自然科学基金(29625305)资助 O643 01050211 WO3/ZrO2固体强酸催化剂上异丁烷/丁烯的烷基化反应:Ⅱ.过渡金属的助催化作用=Study on Alkylation of Isobutane with Butene over WO3/ZrO2 Strong Solid Acid:Ⅱ. Promotions Effect of Transition Metal[刊, 中]/孙闻东(中科院长春应用化学所),吴越∥催化学报.&2000, 21(3).&229~233 制备了一系列用过渡金属活化的WO3/ZrO2固体强酸催化剂,并对其晶型结构、表面状态和酸量进行了表征.图7表2参22 国家自然科学基金(29792076)资助 O212 01050212 超细镍基催化剂上CH4-CO2重整反应的性能:Ⅰ.制备方法对催化剂结构和还原性能的影响=CH4 Reforming with CO2 over Superfine Ni-Based Catalysts:Ⅰ. Effects of Preparation Methods on Structure and TPR of Catalysts[刊, 中]/许峥(清华大学),张鎏∥催化学报.&2000, 21(3).&234~238 国家博士点基金资助 O643 01050213 SO2对Co3O4/Al2O3选择性催化氧化NO的影响=Effect of SO2 on Catalytic Oxidation of NO over Co3O4/Al2O3[刊, 中]/赵秀阁(华东理工大学),肖文德∥催化学报.&2000, 21(3).&239~242 采用流动态原位IR,TPD及XPS技术研究了NO及SO2在Co3O4/Al2O3金属氧化物催化剂表面上的吸附及脱附行为,并与该催化剂上模拟烟道气中NO的选择性催化氧化活性相关联,考察了SO2对该反应过程的影响及作用机理.图5参13 国家自然科学基金(29633030)资助 O643 01050214 Lewis酸对杂多酸催化异丁烷/丁烯烷基化反应的作用=Effect of Lewis Acid on Alkylation of Isobutane with Butene[刊, 中]/赵振波(中科院长春应用化学所),吴越∥催化学报.&2000, 21(3).&243~246 考察了SbCl5对杂多酸(HPA)催化异丁烷/丁烯烷基化反应的修饰作用,用Hammett指示剂测定了SbCl5/HPA催化剂的酸强度.结果表明,经SbCl5修饰的HPA的酸强度有所提高,SbCl5的加入量、反应温度及反应时间对烷基化油收率及产物分布均有不同的影响.图5表2参15 国家自然科学基金(29792076)资助 O643 01050215 微波固相法制备的ZnCl2/Y在α-蒎烯环氧化物重排反应中的催化性能=Catalytic Performance of ZnCl2/Y Catalyst Prepared by Solid-State Microwave Irradiation for Rearrangement Reaction of α-Pinene Oxide[刊, 中]/尹笃林(湖南师范大学),银董红∥催化学报.&2000, 21(3).&251~254 国家自然科学基金(29572042,29972011)资助 O643 01050216 Co-Mo/HZSM-5甲烷无氧芳构化催化剂上的积炭=Characterization of Coke on Co-Mo/HZSM-5 Catalyst for Methane Dehydro-Aromatization in the Absence of Oxygen[刊, 中]/田丙伦(中科院大连化学物理所),徐奕德∥催化学报.&2000, 21(3).&255~258 国家重点基础研究发展规划项目 O643 01050217 热处理改性的活性炭纤维的脱硫活性=Desulfurization Activity of Activated Carbon Fiber Modified by Heat Treatment[刊, 中]/李开喜(中科院山西煤炭化学所),凌立成∥催化学报.&2000, 21(3).&264~268 考察了在O2和水蒸气存在下高温热处理对活性炭纤维(ACF)催化转化SO2为H2SO4的活性的影响,关联了ACF表面含氧官能团的种类及数量与其脱硫活性之间的关系.图4表2参20 国家自然科学基金(29633030)资助 O643 01050218 制备方法对锶改性氧化铝的高温热稳定性的影响=The Influence of Preparation Methods on the Thermal Stability of Alumina Modified by Strontium[刊, 中]/刘勇(复旦大学),陈晓银∥催化学报.&2000, 21(3).&273~275 福特-中国研究与发展基金资助 O643 01050219 NO在氧化铝负载的Pd催化剂上吸附的TPD-MS研究=TPD-MS Study of NO Adsorption on Alumina Supported Pd Catalysts[刊, 中]/刘振林(中国科技大学),伏义路∥催化学报.&2000, 21(3).&279~282 福特-中国研究与发基金资助项目 O643 01050220 不同焙烧气氛对氟化镁表面碱性的影响=The Effect of Different Calcination Atmosphere on the Surface Basicity of Magnesium Oxide[刊, 中]/孟明(中国科技大学),林培琰∥催化学报.&2000, 21(3).&286~288 在不同气氛中600℃下焙烧Mg(OH)2制得了MgO样品.结果表明,Mg(OH)2经焙烧后分解为MgO.样品的比表面积受焙烧气氛的影响,真空中制备的样品比表面积最大,而空气中制备的样品比表面积最小.真空中制备的样品具有最多的碱位和最强的碱性.图3表2参8 国家自然科学基金(29973037)资助 O643 01050221 Ni改性Cu/Mn/ZrO2催化剂上合成低碳混合醇的研究=Synthesis of Mixed Higher Alcohols by CO Hydrogenation over Ni-Modified Cu/Mn/ZrO2 Catalyst[刊, 中]/陈小平(中科院山西煤炭化学所),吴贵升∥催化学报.&2000, 21(4).&301~304 国家自然科学基金(29873064)资助 O643 01050222 超细镍基催化剂上CH4-CO2重整反应的性能:Ⅱ.制备方法对催化和抗积碳性能的影响=CH4 Reforming with CO2 over Superfine Ni-Based Catalysts:Ⅱ.Effects of Preparation Methods on Reactivity and Carbon Deposition over Catalysts[刊, 中]/许峥(清华大学),张鎏∥催化学报.&2000, 21(4).&309~313 国家博士点基金资助 O643 01050223 合成甲基叔丁基醚的分子筛催化剂研究=A Study of Zeolite Catalysts for Synthesis of Methyl tert-Butyl Ether[刊, 中]/李永红(天津大学),王莅∥催化学报.&2000, 21(4).&323~326 中国石油化工集团公司资助 O643 01050224 二苯并噻吩在CoMoNx催化剂上的加氢脱硫=Hydrodesulfurization of Dibenzothiophene over Cobalt-Promoted Molybdenum Nitride Catalysts[刊, 中]/柳云骐(石油大学),刘晨光∥催化学报.&2000, 21(4).&337~340 中国石油天然气集团公司资助 O643 01050225 锆助剂含量对钴基费-托合成催化剂的影响=Effect of ZrO2 Loadings on Catalytic Performance of Co/SiO2 for F-T Synthesis[刊, 中]/陈建刚(中科院山西煤炭化学所),相宏伟∥催化学报.&2000, 21(4).&359~362 国家重点基础研究发展规划项目 O643 01050226 一种天然气直接转化制乙烯的新过程=A Novel Process for Direct Conversion of Natural Gas to Ethylene[刊, 英]/朱爱民(中科院大连化物所),田志坚∥催化学报.&2000, 21(4).&395~396 国家科技部资助项目 O643 01050227 N,N-二甲基胺乙基膦酸-磷酸氢锆为载体的新型固体碱研究=Study on Solid Base Supported on Zirconium Dimethyl Aminoethylphosphonate-Phosphate[刊, 中]/龚成斌(中科院大连化学物理所),曾仁权…∥应用化学.&2000, 17(3).&264~267 国家自然科学基金资助 O643 01050228 丙烷在钒-磷混合氧化物催化剂上晶格氧氧化及其循环流化床工艺=Oxidation of Propane over Vanadium-Phosphorus Oxides Catalyst by Lattice Oxygen and Its Circulating Fluidized Bed Process[刊, 中]/王鉴(浙江大学),赵如松…∥应用化学.&2000, 17(3).&313~315 用脉冲色谱研究了丙烷在钒-磷混合氧化物催化剂上的选择氧化反应,并结合TPD和XRD表征结果,证明丙烷是与催化剂的晶格氧反应生成丙烯酸和乙酸.图2参5 国家自然科学基金(29676007)资助 O643 01050229 用以分解氟里昂-12的新型催化剂WO3/Al2O3=A Novel Catalyst WO3/Al2O3 for Decomposition of CFC-12[刊, 中]/马臻(复旦大学),华伟明…∥应用化学.&2000, 17(3).&319~321 国家科委攀登计划资助 O643 01050230 LaFeO3的光催化性=Photocatalytic Activity of LaFeO3[刊, 中]/白树林(天津大学),付希贤…∥应用化学.&2000, 17(3).&343~345 采用柠檬酸法和微乳液法制备纳米晶LaFeO3.结果表明,微乳液法较柠檬酸法制备的LaFeO3粒径小,表面积大,使LaFeO3吸附性增加,因此表现出较强的光催化活性.图2表1 国家自然科学基金(59772019)资助 O643 01050231 NiO/SiO2催化剂上CO氧化的反应机理研究=Mechanism of CO Oxidation on NiO/SiO2 Catalyst[刊, 中]/王远洋(宁夏大学),高荫本…∥宁夏大学学报(自然科学版).&2000, 21(2).&147~149 NiO/SiO2是优良的非贵金属型CO氧化催化剂,EXAFS和TPR表征表明其活性中心为NiO.在此基础上的Monte Carlo模拟表明,在NiO/SiO2上CO氧化遵循L-H机理.图5参11 国家自然科学基金(29803009)资助 O643 01050232 烯基取代的茂钛族络合物合成及应用于烯烃催化聚合的进展=Recent Advances in the Synthesis of Alkenyl Substituted Metallocene Complexes(Ti,Zr,Hf) and the Application of Its to α-Olefins Polymerization[刊, 中]/钱贤苗(华东理工大学),许胜…∥功能高分子学报.&2000, 13(3).&358~366 综述了含烯基取代基的茂钛族络合物的合成,取代基上双键的化学反应,以及在α-烯烃催化聚合上的最新进展.表2参45 国家自然科学基金(29871010)资助 O643 01050233 氧化态Co/γ-Al2O3催化剂的结构与反应性能研究:Ⅱ.钴物种微观结构的XAFS表征=Studies on the Structures and Catalytic Properties of Oxidized Co/γ-Al2O3 Catalysts:Ⅱ.XAFS Characterizations of the Micro-Structures of Co Species[刊, 中]/孟明(中国科学技术大学),林培琰…∥分子催化.&2000, 14(3).&161~165 采用X-射线吸收近边结构与扩展X-射线吸收精细结构技术,对用不同原料盐和不同焙烧温度制得的Co/γ-Al2O3催化剂中钴的微观结构进行了详细的表征.图5表1参8 国家自然科学基金(29973037)资助 O643 01050234 CuCl/γ-Al2O3上NO脱除过程研究=Reaction Mechanism of NO Conversion over CuCl/γ-Al2O3[刊, 中]/朱月香(北京大学),尉继英…∥分子催化.&2000, 14(3).&166~170 设计了一系列的对比实验,利用XRD、XPS、TPR、EXAFS等技术,系统研究了CuCl/γ-Al2O3上的NO脱除反应过程.图4表1参8 国家自然科学基金(29733080)资助 O643 01050235 以Au(PPh3)(NO3)为前体的Au/NiO催化剂的制备及其对CO的催化氧化=Preparation and Catalytic Performance of Au/NiO Catalyst Derived from Au(PPh3)(NO3) for CO Oxidation[刊, 中]/邹旭华(烟台大学),齐世学…∥分子催化.&2000, 14(3).&171~174 采用有机金属配合物固载法,将金的有机配合物Au(PPh3)(NO3)沉积于刚制备出的Ni(OH)2沉淀上.结果表明,以K2CO3作为制备Ni(OH)2的沉淀剂,金担载量为3%(质量分数)在焙烧温度为300℃的条件下,制备出的负载型金催化剂Au/NiO对CO的低温氧化有很高的催化活性.图2表3参15 国家自然科学基金(29873041)资助 O643 01050236 CexZr1-xO2复合氧化物负载PdO催化剂的CO和CH4氧化性能研究=CO and Methane Oxidation over CexZr1-xO2 Mixed Oxide Supported PdO Catalysts[刊, 中]/马磊(浙江大学),韩立峰…∥分子催化.&2000, 14(3).&175~178 浙江省自然科学基金资助 O643 01050237 溶胶-凝胶法镍基催化剂的研究&&Ti/Si原子比对NiO-TiO2-SiO2结构和苯加氢性能的影响=Study of the Nickel Catalysts Prepared by Sol-Gel Method&&The Effects of Si/Ti Atomic Ratios on the Structure and Catalytic Performance for Benzene Hydrogenation of NiO/TiO2-SiO2[刊, 中]/朱毅青(江苏石油化工学院),林西平…∥分子催化.&2000, 14(3).&184~190 中石化集团公司资助课题 O643 01050238 (NH4)15[La(PMo9V2O39)2]*6H2O杂多配合物对苯酚羟化的催化作用=Catalytic Activity of (NH4)15[La(PMo9V2O39)2]*6H2O Heteropoly Complex in Phenol Hydroxylation with Hydrogen Peroxide[刊, 中]/林深(福建师范大学),郑瑛…∥分子催化.&2000, 14(3).&191~194 福建省科委资助项目 O643 01050239 Co改性Mo/HZSM-5催化剂上甲烷无氧芳构化反应研究=Methane Dehydro-Aromatization over Cobalt Modified Mo/HZSM-5 Catalysts in the Absence of Oxygen[刊, 中]/田丙伦(中科院大连化学物理所),刘红梅…∥分子催化.&2000, 14(3).&200~204 国家重点基础研究发展规划项目 O643 01050240 甲醇催化氧化重整反应制氢的研究=Hydrogen Production by Catalytic Oxidation and Reforming of Methanol[刊, 中]/陈兵(华南理工大学),董新法…∥分子催化.&2000, 14(3).&205~208 广东省高教厅自然科学基金资助 O643 01050241 TiO2催化Cr(Ⅵ)-氯代苯酚共存体系中Cr(Ⅵ)的光致还原=Photo-Induced Cr(Ⅵ) Reduction in Cr(Ⅵ)-4CP Coexist Pollution System in the Presence of TiO2 Photocatalyst[刊, 中]/付宏祥(中科院兰州化学物理所),吕功煊…∥分子催化.&2000, 14(3).&214~218 中科院重大项目资助 O643 01050242 三磺化三苯基膦(TPPTS)制备方法的改进=Improvement of Synthesis Method of TPPTS[刊, 中]/张敬畅(北京化工大学),曹维良…∥分子催化.&2000, 14(3).&223~226 国家"九五”科技攻关项目 O644 01050243 预处理对TiO2表面超亲水性的影响=Effect of Pretreatment on Superhydrophilicity of TiO2 Films[刊, 中]/管自生(中科院化学所),马颖∥感光科学与光化学.&2000, 18(3).&204~207 在高温度环境下,应用紫外光辐射对溶胶-凝胶TiO2薄膜预处理,经过预处理的薄膜烧结后具有快速光致高度亲水特性和良好的光催化活性.超亲水的薄膜能够吸附大量的酸性品红,在光照下薄膜将吸附的酸性品红逐渐降解掉.图3参6 国家自然科学基金(59932040)重点资助 O644 01050244 荧光探针在测定N-异丙基丙烯酰胺共聚物最低临界溶液温度(LCST)时的扩散过程=The Diffusion Process of Fluorescence Probe in Measurement of LCST of Poly(N-Isopropylacrylamide-co-Acrylamide) Aqueous Solution[刊, 中]/李俊(中科院感光化学所),史向阳∥感光科学与光化学.&2000, 18(3).&208~213 合成了多种不同配比的N-异丙基丙烯酰胺(NIPAM)与丙烯酰胺(AM)的共聚物.研究了共聚物溶液在温度变化过程中发生的线团-胶粒转变,及荧光探针分子扩散进入疏水胶粒的动力学过程.图3表2参9 国家自然科学基金(29733100)资助 O644 01050245 蒽醌冠醚铕离子络合物分子内能量转移=Intramolecular Energy Transfer in Anthraquione Crown Ether-Eu3+ Complex[刊, 中]/江华(中科院感光化学所),许慧君∥感光科学与光化学.&2000, 18(3).&223~228 研究结果表明,选择性激发铕离子络合物中的蒽醌冠醚能发生分子内能量转移,观察到铕离子的发光.荧光衰减测试表明,由于冠醚的包络作用使得铕离子络合物的荧光寿命较未络合铕离子的寿命有所延长.图5表1参10 国家自然科学基金(29733100)资助 O644 01050246 有机染料光褪色机理及主要原因=The Mechanism and Main Reasons of Photofading of Organic Dyes[刊, 中]/于新瑞(大连理工大学),张淑芬∥感光科学与光化学.&2000, 18(3).&243~253 综述了有机染料光褪色机理的主要研究方法以及现有研究结论.对光氧化和光还原过程、影响因素、典型反应步聚、研究手段进行了系统的阐述,同时也对通过染料分子结构设计合成、通过添加剂的使用提高染料光牢度的方法进行了介绍.图4表3参29 国家自然科学基金重点基金(29836150)资助 O644 01050247 荧光分子开关的研究进展=The Progress of the Research on Fluorescent Molecular Switches[刊, 中]/甘家安(华东理工大学),陈孔常∥感光科学与光化学.&2000, 18(3).&254~262 根据开关动作完成的方式对近年来研究的各类荧光分子开关进行了简要的归类总结,并介绍了一些具有特殊功能的荧光分子开关器件.图8参33 国家自然科学基金(29836150)资助 O644 01050248 半花菁衍生物LB膜的光致荧光特性研究=Photoluminescence Characteristics of Hemicyanine Derivatives in Langmuir Blodgett Multilayers[刊, 中]/王文军(复旦大学),陆兴泽…∥物理化学学报.&2000, 16(6).&496~500 通过改变半花菁的亲水基团与硫水基团而得到了四种半花菁衍生物,利用稳态和时间分辨荧光研究了不同亲水基团与疏水基团对半花菁衍生物光学特性的影响.图1表3参7 国家自然科学基金重点基金(19834030)资助 O644 01050249 N-萘甲基多乙撑多胺类荧光化学敏感器=Fluorescence Chemical Sensor of Naphthylmethyl Polyethylenepolyamine[刊, 中]/梅明华(中科院感光化学所),吴世康∥物理化学学报.&2000, 16(6).&559~562 合成了一类带有萘甲基的多乙撑多胺化合物,对其在不同溶剂中作为FCS检测锌离子时的荧光光谱变化进行了研究.结果表明,两种不同锌盐化合物与配体相互作用时,其所带阴离子对络合物的组成和方式有很大的影响.图2表1参6 国家自然科学基金(29733100)资助 O644 01050250 三聚氰胺甲醛树脂的光学性质=Optical Properties of Melamine Formaldehyde Resin[刊, 中]/刘海波(复旦大学),侯占佳…∥物理化学学报.&2000, 16(6).&563~567 对三聚氰胺甲醛树脂的合成过程、波导制备条件及利用三聚氰胺甲醛树脂作为掺杂基质的非线性光学性质进行了研究.实验结果表明,三聚氰胺甲醛树脂是一种极具有应用前景的光学材料.图3参14 国家自然科学基金(69708005,69808001)资助 O644 01050251 紫菜两个光系统间激发能分配研究对光合进化的启示[刊, 中]/潘洁(中科院化学所),施定基…∥科学通报.&2000, 45(12).&1276~1279 选择两个不同发育阶段(孢子体和配子体)的条斑紫菜作为研究对象.对过多激发能在两个光系统间的分配进行了比较研究.图2参13 国家自然科学基金(39890390,29733100)资助 O644 01050252 非线性光学配合物的化学研究[刊, 中]/左景林(南京大学),游效曾∥科学通报.&2000, 45(14).&1457~1464 从化学角度出发,在分子水平上研究和探索新型分子基非线性光学材料是一个新的研究领域.总结了近年来在新型二阶、三阶极化率和光限制等非线性光学配合物的化学研究方面所取得的进展.图6表2参46 国家自然科学基金(29631040)资助 O644 01050253 螺旋共轭化合物1′,3′,3′-三甲基-6-羟基螺旋[2H-1-苯并吡喃-2,2′-二氢吲哚]的结构、光谱、非线性光学性质和光异构化过程的理论研究=Theoretical Study of the Structures, Spectra, Nonlinear Second-Order Optical Properties and Photoisomerization Process of the Spiroconjugated Compound 1′,3′,3′-Trimethyl-6-Hydroxyspiro[2H-1-Benzopyran-2,2′-Indoline][刊, 中]/孙秀云(吉林大学),封继康…∥高等学校化学学报.&2000, 21(7).&1080~1085 用AM1和ZINDO方法研究了螺旋共轭化合物1′,3′,3′-三甲基-6-羟基螺旋[2H-1-苯并吡喃-2,2′-二氢吲哚](HBPS)及其光导构化产物的结构和光谱性质,从理论上研究了HBPS的光异构化反应过程,并在此基础上,用自编的完全态求和公式(SOS)程序计算了非线性光学系数.图4表7参17 国家自然科学基金(29873016)资助 O644 01050254 Af型自由基均聚反应的固化理论(Ⅲ)&&高分子凝胶网络缺陷=The Curing Theory of Af Type Free Radical Homopolymerization(Ⅲ)&&The Defects of Polymer Gel Network[刊, 中]/王海军(河北大学),赵敏…∥高等学校化学学报.&2000, 21(7).&1138~1141 应用高分子反应统计理论,分析了高分子网络的形成过程,讨论了Af型自由基均聚反应凝胶网络中的缺陷&&悬吊环的数目及其形成几率.图4参22 国家自然科学基金(29673018)资助 O646 01050255 微过氧化物酶-11修饰电极对O2和H2O2的电催化还原=Electrocatalytic Reduction of O2 and H2O2 at the Glass Carbon Electrode Modified with Microperoxidase-11[刊, 中]/杨辉(南京师范大学),黄志忠…∥物理化学学报.&2000, 16(6).&527~532 运用电化学循环伏安法和旋转圆盘电极技术研究了O2和H2O2在Nafion膜固定的微过氧化物酶-11修饰的玻碳电极上的电化学还原.图6参16 国家自然科学基金资助 O646 01050256 电沉积Co-Cu颗粒膜的巨磁电阻效应=Giant Magnetoresistance in Electro-Deposited Co-Cu Granular Film[刊, 中]/李浩华(兰州大学),黎超…∥物理化学学报.&2000, 16(6).&573~576 用电化学沉积法制备了Co-Cu纳米颗粒膜,并对这种膜的结构、磁性、巨磁电阻效应作了较详细的研究.图6参7 国家自然科学基金资助 O646 01050257 磷酸溶液中铝恒压阳极氧化的研究=Investigation of Aluminum Anodizing in Phosphoric Acid Solution at Constant Voltage[刊, 中]/王为(天津大学),郭鹤桐…∥天津大学学报.&2000, 33(3).&341~343 结果表明,随着电解电压的升高,形成的氧化铝多孔膜的阻挡层增厚,多孔层的孔径和胞径均增加,其原因与离子迁移等密切相关.图6参6 国家自然科学基金(69601006)资助 O646 01050258 二次微分简易示波伏安法测定痕量镍=Determination of Trace Nickel by Second-Order Differential Simple Oscillographic Voltammetry[刊, 中]/傅业伟(西北大学),高敏…∥应用化学.&2000, 17(3).&296~299 国家自然科学基金(29775018)资助 O646 01050259 Ml(NiCuAlZn)5储氢合金粉末的包覆处理=Encapsulation of Ml(NiCuAlZn)5 Hydrogen Storage Alloy Powder[刊, 中]/王一菁(南开大学),赵东…∥应用化学.&2000, 17(3).&310~312 国家"八六三”计划资助项目 O646 01050260 和厚朴酚电化学还原机理的研究=Theoretical Study on the Mechanism of Electrochemical Reductions Reaction of Honokiol[刊, 中]/孙成科(曲靖师范高等专科学校),龚思源…∥高等学校化学学报.&2000, 21(6).&912~917 用量子化学AM1、PM3方法研究了和厚朴酚分子的电化学还原反应机理.结果表明,和厚朴酚分子电还原的基团为两个烯丙基的双键,其中邻位烯丙基优先被还原,对位次之,整个还原反应中和厚朴酚得到4e和4H+,使两个烯丙基饱和,计算结果较好地说明了实验事实.图2表7参12 国家自然科学基金(29773007)资助 O646 01050261 Fourier自去卷积示波计时电位法的研究=Oscillographic Chronopotentiometry with Fourier Self-Deconvolution[刊, 中]/郑建斌(西北大学),张红权…∥高等学校化学学报.&2000, 21(7).&1037~1039 国家自然科学基金(29775018)资助 O646 01050262 用规整膜板对砷化镓的三维微结构图形加工刻蚀=Three-Dimensional Microfabrication on GaAs Using a Regular Patterns Mold[刊, 中]/董海苟(厦门大学),孙建军…∥电化学.&2000, 6(3).&253~257 以微齿轮图形结构作为规整模板.用约束刻蚀剂层技术对GaAs样品表面进行了加工刻蚀.在有捕捉剂H3AsO3存在的情况下,规则微齿轮图形能够很好地在样品表面复制.图5参6 国家自然科学基金重点项目(29833070)资助 O646 01050263 混凝土中钢筋的腐蚀行为研究=Corrosion Behavior of Reinforcing Steel in Concrete[刊, 中]/林荣归(厦门大学),胡融刚…∥电化学.&2000, 6(3).&305~310 钢筋在混凝土中受到高碱性环境的保护,耐蚀性好,但随着介质pH的降低、Cl-含量的上升,钢筋腐蚀电流升高;动电位阳极极化曲线的测试表明,钢筋的维钝电流增大,击穿电位负移,混凝土中钢筋的耐蚀性下降.图4表1参6 国家自然科学基金(59871043)资助 O647 01050264 天冬氨酸在Cu(001)表面吸附的扫描隧道显微镜研究=Adsorption of Aspartic Acid on Cu(001) Studied by Scanning Tunneling Microscopy[刊, 中]/王浩(北京大学),赵学应…∥物理学报.&2000, 49(7).&1316~1320 国家自然科学基金(19634010)资助 O647 01050265 共聚高分子吸附的Monte Carlo模拟=Monta Carlo Simulation of Adsorption of Diblock Copolymer at Solid-Liquid Interface[刊, 中]/陈霆(华东理工大学),刘洪来…∥化工学报.&2000, 51(4).&512~518 用Monte Carlo方法对选择性溶剂中两嵌段共聚高分子在固液界面的吸附进行了模拟,获得了吸附等温线以及吸附层厚度、链附着率、表面覆盖率、链节浓度分布等表征吸附层结构的信息,同时模拟获得了固液界面区吸附构型大小及分布等表征高分子构型的微观信息.图12表1参10 国家自然科学基金(29736170)资助 O647 01050266 无规共聚高分子溶液相平衡的Monte Carlo模拟=Monte Carlo Simulations of Phase Equilibria of Random Copolymer Solutions[刊, 中]/陈霆(华东理工大学),刘洪来…∥化工学报.&2000, 51(4).&552~554 用构型偏倚蒸发Monte Carlo方法模拟研究了密堆积格子模型上A、B无规共聚高分子的相平衡问题,无规共聚高分子中链节A、B以不同的相互作用能来区别.模拟获得了链长直至200个链节的无规共聚高分子系统相平衡数据,同时计算获得了不同链长固定组成下无规共聚高分子系统相应的临界点.图2表1参12 国爱自然科学基金(29736170)资助 O648 01050267 Triton X-100/正癸醇/水层状液晶制备泡囊=Preparation of Vesicles from Lamellar Liquid Crystal in Triton X-100/n-C10H21OH/H2O System[刊, 中]/丁元华(扬州大学),刘天晴…∥物理化学学报.&2000, 16(6).&481~486 以Triton X-100/n-C10H21OH/H2O体系生成的层状液晶为介质制备泡囊,所得泡囊分布比较均匀,囊径在0.2~0.5 μm之间.研究了层状液晶组成、pH、超声振荡时间等因素对所制泡囊的电位及微极性的影响.图6表2参16 国家自然科学基金(29673035)资助 O648 01050268 表面波技术研究TBP对C12E8表面流变性质的影响=Influence of TBP on the Surface Rheological Properties of C12E8 Studied by Surface Wave Method[刊, 中]/韩国彬(厦门大学),吴金添…∥物理化学学报.&2000, 16(6).&507~511 利用表面波技术研究消泡剂TBP对起泡剂C12E8表面流变性质的影响,阐述泡沫的排液过程中液膜变形所产生的表面张力梯度修复机理以及液膜强度与表面膨胀模量、表面膨胀弹性和表面膨胀粘度的关系,并且探讨了消泡剂TBP的消泡机理.图4参6 国家自然科学基金(29973033)资助 O648 01050269 制备宽温区O/W微乳的新方法=Novel Method to Prepare O/W Microemulsions within a Wide Temperature Range[刊, 英]/梁文平(中科院化学所),Tharwat F Tadros∥物理化学学报.&2000, 16(6).&538~542 通过把一个带有羧基的离子表面活性剂镶嵌在微乳界面的单分子层中,在低pH值时,形成水包油微乳,然后用氢氧化钠中和使其表面离子化而形成一个较厚的双电层,从而得到的宽温区(0-90℃)水包油微乳.研究了油相浓度、pH以及微乳液滴的大小对体系的浊点的影响.图5参12 国家自然科学基金资助 O65 01050270 Triton X-100与牛血清白蛋白的相互作用=The Interaction between Triton X-100 and Bovine Serum Albumin[刊, 中]/魏晓芳(中科院化工冶金所),刘会洲∥分析化学.&2000, 28(6).&699~701 国家自然科学基金重点项目资助 O65 01050271 球形铜离子模板缩聚物的制备及其选择吸附性能=The Preparation of Globular Copper(Ⅱ) Template Condensation Polymer and Its Behavior of the Selective Adsorption[刊, 中]/王旭东(南开大学),何锡文…∥分析化学.&2000, 28(7).&805~809 以苯酚、甲醛、四乙烯五胺为反应单体,铜离子为模板,反相悬浮聚合制备出球形离子模板缩聚物.该模板缩聚物对铜离子的吸附性能显著优于相应的非模板缩聚物.图6参3 国家自然科学基金(29775011)资助 O65 01050272 杯芳烃银离子敏感场效应晶体管传感器=Silver Ion-Sensitive Field-Effect Transistor Based on Calixarene[刊, 中]/陈朗星(南开大学),牛文成…∥分析化学.&2000, 28(7).&815~819 报道了硫醚取代的杯[4]芳烃化合物的合成,以此化合物为敏感材料,研制了聚氯乙烯膜的银离子敏感场效应晶体管(Ag+-ISFET)传感器.Ag+-ISFET传感器对银离子表现出优良的能斯特响应,该传感器对碱金属、碱土金属及过渡金属离子有极高的选择性.图5表1参13 国家自然科学基金(29975014)资助 O65 01050273 电极扩散层内物质浓度及分布研究方法进展=Development of Research Method of Concentration Observation and Distribution in Electrode Diffusion Layer[刊, 中]/杨玉书(重庆大学),夏之宁…∥分析化学.&2000, 28(7).&897~903 评述了测试电极扩散层内物质浓度及分布的各种方法,分别从方法原理、实验装置、影响因素及其应用等方面加以论述,评价了各种方法的特点,并对发展方向进行了展望.参52 国家自然科学基金(29775035)资助 O657 01050274 甲烷氧化偶联Na-W-Mn/SiO2催化剂的喇曼光谱=Raman Spectroscopy Characterization of Na-W-Mn/SiO2 Catalyst for Oxidative Coupling of Methane[刊, 中]/陈宏善(中科院兰州化学物理所),牛建中…∥物理化学学报.&2000, 16(6).&543~546 研究了A16061/SiCp复合材料表面双层稀土转化膜的微观结构以及化学组成,并对稀土转化膜对抗腐蚀性能进行了评估.结果表明,稀土转化膜能显著提高耐蚀性.图2表3参10 国家自然科学基金资助 O657 01050275 12-钼磷酸-葡聚糖包合物的研究=Study on the Inclusion Compound of 12-Molybdenum Phosphoric Acid with Dextran[刊, 中]/程贤C026(福建师范大学),苏英草…∥物理化学学报.&2000, 16(7).&658~662 福建省教委基金资助 O657 01050276 O/W微乳液中制备甲基丙烯甲酯-苯乙烯共聚物超细颗粒=Preparation of Methyl Methacrylate and Styrene Copolymer Ultrafine Particles in the Oil-in-Water Microemulsions[刊, 中]/钱君律(同济大学),甘礼华…∥同济大学学报(自然科学版).&2000, 28(3).&320~323 在甲基丙烯酸甲酯(MMA)-苯乙烯-环己烷/十六烷基三甲基溴化铵/水体系的O/W微乳液中,制备了粒径约58 nm球状甲基丙烯酸甲酯-苯乙烯共聚物超细颗粒.研究了影响粒子形态和大小的因素.图4表1参12 国家自然科学基金(29673030)资助 O657 01050277 基于小波神经网络的新型算法用于化学信号处理=A Novel Algorithm Based on the Wavelet Neural Network for Processing Chemical Signals[刊, 中]/蔡文生(中国科技大学),于芳…∥高等学校化学学报.&2000, 21(6).&855~859 基于紧支集正交小波神经网络的构造思想,用具有紧支集的B-样条函数的伸缩和平移替代小波函数,提出了一种新型算法,并将其应用于化学信号的处理,实现了信号的压缩和滤噪,与自适应小波神经网络相比,学习速度得到了大幅度的提高.图4表2参9 国家自然科学基金(29775001)资助 O657 01050278 改进溶胶-凝胶法固定酶结构剖析及在苯酚光化学传感器中的应用=The Microstructure Analysis of Enzymes Immobilized by Sol-Gel Method and Its Application in Phenol Optical Chemical Sensor[刊, 中]/李军(湖南大学),王柯敏…∥高等学校化学学报.&2000, 21(7).&1018~1022 结果表明,酶固定于溶胶-凝胶中后,与在溶液中一样,呈均匀分布且不易流失.此敏感膜可用于制备基于化学发光强度减弱的苯酚光化学传感器;用竞争反应的原理讨论了响应机理.图3表1参20 国家自然科学基金(29675005)及国家杰出青年基金(29825110)资助 O657 01050279 一种用于电热蒸发-原子光谱的微型化固相萃取技术=A Miniaturization Solid-Phase Extraction Technique Designed for Electrothermal Vaporization-Atomic Emission Spectrometry Determination[刊, 中]/熊宏春(武汉大学),胡斌…∥高等学校化学学报.&2000, 21(7).&1035~1036 国家自然科学基金(29875022)资助 O657 01050280 多环芳烃芴、苊的无保护流体室温磷光性质研究=Studies on Non-Protected Fluid Room Temperature Phosphorescence of Polycyclic Aromatic Hydrocarbon of Fluoren and Acenaphthene[刊, 中]/李隆弟(清华大学),牟兰…∥高等学校化学学报.&2000, 21(7).&1040~1042 国家自然科学基金(29775013)资助 O657 01050281 高效液相色谱/大气压化学电离质谱快速分析水中痕量有机磷农药=Rapid Analysis of Trace Organophosphorous Pesticides in Water by High Performance Liquid Chromatography/Atmospheric Pressure Chemical Ionization Mass Spectrometry[刊, 中]/潘元海(中科院生态环境研究中心),金军…∥分析化学.&2000, 28(6).&661~671 用反相液谱短柱实现了6种有机磷农药(乙酰甲胺磷,甲基对硫磷、杀螟硫磷、喹硫磷、二嗪农、辛硫磷)的高效液相色谱/大气压化学电离质谱(HPLC/APCIMS)快速分析.此技术可以很好地实现水中痕量有机磷农药的测定,一次联机分析仅需10 min.图3表2参11 国家自然科学基金(29977023)资助 O657 01050282 氧氟沙星对映体的同步-导数荧光光谱法识别与测定=Recognition and Determination of Ofloxacin Enantiomers by Synchronization-Derivative Fluorimetry[刊, 中]/弓巧娟(山西大学),晋卫军…∥分析化学.&2000, 28(6).&672~677 控制pH=3,首次通过同步一阶导数荧光光谱技术,有效地识别和分辨氧氟沙星的R和S对映体,同时有效地消除尿样背景的干扰.据此建立了同时识别与测定两种旋光体的同步-导数荧光光谱法.图5表2参9 国家自然科学基金(29875016)资助 O657 01050283 一种新的重原子微扰剂氯代叔丁烷在亚硫酸钠化学除氧环糊精诱导室温磷光法中的应用研究=A Study of t-Butyl Chloride as Heavy Atom Perturber in Chemical Deoxygenation-Cyclodextrin Induced Room Temperature Phosphorimetry[刊, 中]/张淑珍(山西大学),魏雁声…∥分析化学.&2000, 28(6).&678~681 以氯代叔丁烷作重原子微扰剂采用亚硫酸钠化学除氧技术建立了菲、7,8-苯并喹啉、2-溴代萘在环糊精介质中的室温磷光分析法.图4表2参5 国家自然科学基金(29575204)资助 O657 01050284 小牛胸腺脱氧核糖核酸诱导中性红聚集的机理及其影响因素=The Aggregation of Neutral Red Encouraged by Deoxyribonucleic Acids and Its Depending Factors[刊, 中]/黄新华(西南师范大学),李原芳…∥分析化学.&2000, 28(6).&682~686 在pH7.6~7.8和低离子强度条件下,中性红与小牛脱氧核糖核酸(ctDNA)作用产生以535 nm为特征的共振光散射增强(ERLS)光谱.机理研究表明,这种ERLS是由于DNA诱导中性红的聚集所致.图6表1参11 国家自然科学基金(29875019)资助 O657 01050285 蛋白质样品的非竞争性毛细管电泳免疫分析激光诱导荧光检测=Noncompetitive Capillary Electrophoresis Based Immunoassay for Proteins with Laser Induced Fluorescence Detection[刊, 中]/王清刚(清华大学),罗国安…∥分析化学.&2000, 28(6).&687~691 国家自然科学基金重点资助 O657 01050286 亲和毛细管电泳测定孕酮与其单克隆抗体的结合常数=Determination of the Binding Constant between Progesterone and Its Monoclonal Antibody Using Affinity Capillary Electrophoresis[刊, 中]/王清刚(清华大学),王义明…∥分析化学.&2000, 28(6).&731~734 采用亲和毛细管电泳的配体分离模式,以激光诱导荧光作为检测手段,测定了荧光素标记的孕酮与孕酮单克隆抗体之间的结合常数,并研究了温育时间、电泳条件等因素对测定的影响.图3参5 国家自然科学基金重点资助 O657 01050287 钌(Ⅱ)-联吡啶-铈(Ⅳ)-戊二醛化学发光体系及其应用=Chemiluminescence System of Ru(bpy)2+3-Ce(Ⅳ)-Glutaraldehyde and Its Application[刊, 中]/韩鹤友(武汉大学),何治柯…∥分析化学.&2000, 28(6).&738~740 基于在硫酸介质中戊二醛增强铈(Ⅳ)氧化钌(Ⅱ)-联吡啶的化学发光强度.在优化后的条件下,方法的线性范围和检测限分别为2.5×10-6~2.5×10-3 mol/L和3.1×10-7 mol/L戊二醛.图1表1参6 国家自然科学基金(29605001)资助 O657 01050288 毛细管区带电泳法测定甲氧苄胺嘧啶和磺胺甲基异C023唑的离解常数及其在复方新诺明中的含量=Determination of Dissociation Constants of Trimethoprim and Sulfamethoxazole and Their concentrations in Co-Trimoxazole Tablets by Capillary Zone Electrophoresis[刊, 中]/李前锋(兰州大学),张红医…∥分析化学.&2000, 28(7).&793~797 国家自然科学基金(29475194和29675007)资助 O657 01050289 联用色谱数据的半自动解析法=Semiautomatic Deconvolution of Hyphenated Chromatographic Data[刊, 中]/甘峰(湖南大学),李晓宁…∥分析化学.&2000, 28(7).&833~836 采用正交投影法获得初始迭代光谱,继采用非负最小二乘法和常规最小二乘法进行交替迭代,最终获得纯光谱和纯色谱向量.图2参15 国家自然科学基金(29875007)资助 O657 01050290 陈皮挥发油的气相色谱/质谱分析=Determination of the Volatile Oil of Pericarpium Citri Reticulatae with Gas Chromatography/Mass Spectrometry[刊, 中]/龚范(湖南大学),梁逸曾…∥分析化学.&2000, 28(7).&860~864 用GC/MS分析复方制剂平胃散中的一种单味药材陈皮的挥发油化学成分及其相对含量.分离出53个组分,其中鉴定了34个组分,约占其挥发油总量的96.08%.图5表1参7 国家自然科学基金(29875007)资助 657 01050291 人工神经网络法鉴别红外光谱=Artificial Neural Network for the Identification of Infrared Spectra[刊, 中]/李燕(南京理工大学),王俊德…∥光谱学与光谱分析.&2000, 20(4).&477~479 将反向传播人工神经网络用于FTIR,鉴别未知化合物.结果表明,当训练集样本不含噪声时,纯光谱的预测结果很好.而当训练集样本有少量噪声干扰时,预测结果随预测集样本的不同,而得到了不同的改善.图3参6 国家自然科学基金资助 O657 01050292 苯丙氨酸桥联金属双卟啉的诱导圆二色光谱研究=The Study of Induced Circular Dichroism of Phenylalanine Bridged Metal Bis-Porphyrin[刊, 中]/刘海洋(华南理工大学),黄锦汪…∥光谱学与光谱分析.&2000, 20(4).&495~497 报道了邻位和对位桥联苯丙氨酸锌双卟啉的合成,考查了双卟啉的分子结构对诱导圆二色性的影响.结果表明,两个卟啉环之间的π-π堆积和手性激子偶合作用是影响此类双卟啉圆二色光谱的主要因素.图3参16 国家自然科学基金(29771034)资助 O657 01050293 砷和汞的顺序注射-蒸气发生原子吸收光谱测定=Sequential Injection Vapor Generation Atomic Absorption Spectrometric Determination of Mercury and Arsenic[刊, 中]/马泓冰(东北大学),徐淑坤…∥光谱学与光谱分析.&2000, 20(4).&529~532 建立了砷和汞的顺序注射-蒸气发生原子吸收光谱测定方法.在流动注射蒸气发生原子吸收光谱测定方法的基础上,采用汇流技术,通过两个微量注射泵和一个多位选择阀的计算机程控操作,实现待测组分与硼氢化钠间的强氧化还原反应及其气-液分离过程.图3表4参4 国家自然科学基金资助 O657 01050294 氢化物的气相富集及其在超痕量分析中的应用=The Gaseous Phase Enrichment Techniques in Hydride Generation (Review)[刊, 中]/郭旭明(厦门大学),黄本立…∥光谱学与光谱分析.&2000, 20(4).&533~536 从富集原理及其在光谱超痕量分析的应用方面分别对这几种氢化物发生后的气相富集技术进行了较详细地综述.这些气相富集方法包括液氮冷却捕集技术、气球收集技术、石墨炉原位捕集技术、吸收液吸收技术及固体吸附技术等.参63 国家自然科学基金资助 O657 01050295 pH诱导牛血清白蛋白芳香氨基酸残基微环境变化的光谱分析=Spectral Study of the Microenviroment Change of Aromatic Amino-Acid Residues in BSA Induced by pH[刊, 中]/魏晓芳(中科院化工冶金所),刘会洲…∥光谱学与光谱分析.&2000, 20(4).&556~559 用荧光光谱和紫外吸收光谱研究了pH=2.3~11.3范围内牛血清白蛋白芳香氨基酸残基微环境的变化,以此推断蛋白质构象的变化,并讨论蛋白质表面疏水性变化的趋势.图4表1参8 国家自然科学基金重点基金资助 O657 01050296 传感器几何结构对近红外组织氧合检测仪灵敏度的影响=The Influence of Probe Geometry on the Sensitivity of Tissue Oximeter Using Near Infra-Red Spectroscopy[刊, 中]/王峰(清华大学),丁海曙…∥光谱学与光谱分析.&2000, 20(4).&585~588 利用蒙特卡罗仿真方法对光在多层生物组织模型中的平均穿透深度、光子平均飞行距离和空间灵敏度分布进行了仿真,结果表明,传感器几何结构,即光源与光检测器之间的距离是影响灵敏度的重要因素,适当增加这一距离有助于深层组织生化信息的提取和测量灵敏度的提高,但是它又受到信噪比降低的限制.图8参3 国家自然科学基金资助 O657 01050297 精密气动液压伺服系统用于傅里叶变换光谱仪光谱测量=Spectral Measuring of Fourier Transform Spectrometer Using Pneumatic Hydraulic Servo System[刊, 中]/李志刚(中科院长春光学精密机械所),王淑荣…∥光谱学与光谱分析.&2000, 20(4).&589~591 讨论了傅里叶变换光谱仪动镜稳定平移的重要性,研制了以高压无氧氮气为动力源的精密气动液压系统用于推进在研傅里叶变换光谱仪"猫眼”动镜系统,并在此基础上测量了ZYGO干涉仪光源光谱图.图4参5 国家自然科学基金资助 O658 01050298 超临界流体色谱法分离手性化合物的进展=Advances in Chiral Separations by Supercritical Fluid Chromatography[刊, 中]/任其龙(浙江大学),苏宝根…∥分析化学.&2000, 28(6).&772~776 国家自然科学基金(29776040)资助 O658 01050299 长江口水体混合过程中溶解态稀土元素分布特征[刊, 中]/王中良(中科院地球化学所),刘丛强∥科学通报.&2000, 45(12).&1322~1326 采用液-液萃取与反萃取分离富集方法和ICP-MS测试技术,对长江河口南支盐度0.15~19 mg/g表层水体中溶解态稀土元素的浓度进行了测定.图2表1参17 国家杰出青年科学基金(49625304)资助 O658 01050300 分子烙印手性固定相分离过程热力学研究=Thermodynamic Study on Separation of Molecular Imprinting Chiral Stationary Phase[刊, 中]/王进防(中科院大连化学物理所),周良模…∥高等学校化学学报.&2000, 21(6).&930~933 结果表明,分子烙印手性固定相的分离过程为焓控制过程.随着温度的升高,样品的容量因子降低,手性选择因子α减小.样品与分子烙印固定相的作用力包括非特异作用力和特异作用力,只有特异作用力的差值才是手性分离的本质.表2参22 国家自然科学基金(29575212)资助 O658 01050301 十八烷基醚型高效液相反相色谱填料的制备=Synthesis and Characterization of Ether-Bond RP-Packing[刊, 中]/喻昕(中科院化学所),赵睿…∥高等学校化学学报.&2000, 21(7).&1043~1044 国家自然科学基金(29635010)资助