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Sample records for beauveria bassiana para

  1. Evaluación de Beauveria bassiana para el combate de insectos plaga en maíz almacenado

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    Juan Diego Molina

    2000-01-01

    Full Text Available El objetivo de este estudio fue evaluar la patogenicidad de aislamientos de Beauveria bassiana, para controlar los insectos plaga Prostephanus truncatus y Stophilus zeamais, en mazorcas de maíz almacenado. Se evaluó también la patogenicidad del hongo sobre Teritriosoma nigrescens, depredador natural de P. truncatus. Se mezclaron conidios secos del hongo con kaolinita y se aplicaron a trojas de maíz de 450 mazorcas acomodadas en capas de 50. Se montaron dos ensayos, en el primero se evaluaron aplicaciones del hongo al momento de almacenar el maíz, y en el segundo se evaluaron aplicaciones del hongo realizadas después de almacenar el maíz. La mortalidad de P. truncatus fue cuatro veces mayor en el ensayo donde se aplicó el hongo en forma preventiva. La mortalidad de S. zeamais fue el doble en el tratamiento donde se aplicó el hongo al momento de inocular con P. truncatus. Los niveles de mortalidad por B. bassiana fueron mayores en P. truncatus que en S. zeamais, pero S. zeamais, pero este último mostró un 20% más de infección que el primero

  2. Selection of Beauveria bassiana and Metarhizium anisopliae isolates for the control of Blissus antillus (Hemiptera: Lygaeidae Seleção de isolados Beauveria Bassiana e Metarhizium Anisopliae para o controle de Blissus Antillus

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    Richard Ian Samuels

    2004-06-01

    Full Text Available Chinch bugs of the genus Blissus (Hemiptera: Lygaeidae are serious pests of maize, wheat, sorghum and various grasses. This study is the first to screen isolates of Beauveria bassiana and Metarhizium anisopliae against 4th instar and adult Blissus antillus aiming to develop a biological control program for this important pasture pest. Ten fungal isolates were initially screened and three isolates were chosen for further investigation. To determine virulence, insects were inoculated by immersion in concentrations of 5 ´ 10(8 conidia mL-1. Mortality was evaluated for 10 days. B. bassiana ARSEF 792 was the most virulent isolate to both nymphs and adults, causing 53 and 78% infection, respectively, and values for LT50 of 7.8 and 5.0 days, respectively. Germination studies were carried out to confirm viability and determine speed of germination as a pathogenicity factor. The production of conidia on the cadavers of insects infected with the three selected isolates was determined. The production of conidia on rice media was also evaluated. B. bassiana CG 24 produced the highest number of conidia on insects cadavers (14.9 ´ 10(7 conidia per insect and also on rice media (10.6 ´ 10(9 conidia per g.Percevejos do gênero Blissus (Hemiptera: Lygeidae são pragas importantes das plantações de milho, trigo e sorgo e de vários tipos de gramíneos forrageiros. Esse estudo é o primeiro a amostrar isolados de Beauveria bassiana e Metarhizium anisopliae contra aos 4(0 instares e adultos de Blissus antillus com a finalidade de desenvolver um programa de controle biológico dessa importante praga de pastagens. Dez isolados foram inicialmente testados e três foram escolhidos para maiores investigações. Para determinar a virulência, os insetos foram inoculados por imersão em suspensões de conídios a uma concentração de 5 ´ 10(8 conídios mL-1. Mortalidade foi avaliada por 10 dias. B. bassiana ARSEF 792 foi o isolado mais virulento para as ninfas e os

  3. Evaluación de un biorreactor de lecho empacado para el desarrollo del hongo Beauveria bassiana

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    Zapata Zapata Arley David

    2003-06-01

    Full Text Available La evaluación del biorreactor de lecho empacado para el desarrollo del hongo Beauveria bassiana se hizo con base en las variables temperatura y concentración de conidias, utilizando un sustrato sólido constituido por trigo entero. Se tomaron como parámetros de operación, la altura del lecho, el flujo de aire y la posición dentro del biorreactor, siendo el tiempo de fermentación un parámetro predeterminado que permitió monitorear las variables y comparar la productividad en el desarrollo del hongo. Se pudo observar, con ayuda de un diseño de experimentos factorial y una prueba de Tukey, que bajo ciertas condiciones de operación como una relación H/D = 0.9 (H: altura del lecho, D: diametro del biorreactor y un flujo de aire de 12.3 L/min, se incrementó significativamente la productividad del hongo con respecto al método tradicional de producción (botellas bajo la escala ensayada, siendo la posición un factor poco significativo en el proceso. Palabras clave: Fermentación en estado sólido, control biológico.

  4. Estudio de las condiciones de mezclado en fermentador para la producción de blastosporas de Beauveria bassiana

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    Cipriano García Gutiérrez

    2013-12-01

    Full Text Available Título en ingles: Study of the mixing conditions in bioreactor for blastospores production of Beauveria bassiana.Resumen: Se caracterizaron tres fermentadores: New Brunswick M-19 de 14 litros, Applikon Biocontroller 1035 de 7 litros y New Brunswick Bioflo III de 7 litros, determinando el coeficiente volumétrico de transferencia de oxígeno (KLa, la retención de gas (RG y el tiempo de mezclado (tM. El fermentador New Brunswick Bioflo III tuvo los mejores valores con una relación de diámetro del impulsor/diámetro del tanque (DI/DT de 0.43, KLa = 9.5-208 h-1 y tM = 1.0-3.0 s, por lo que fue seleccionado para realizar la producción de blastosporas de Beauveria bassiana, utilizando melaza como fuente de carbono. Se estudiaron las condiciones de mezclado, utilizando diferentes combinaciones de impulsores tipo Rushton, Maxflo y Lightnin, bajo un diseño experimental factorial 32. El tiempo de propagación fue de 4 días, el volumen de trabajo 4 litros, 10% de inóculo (1x106 blastosporas/ml, temperatura 30°C, agitación de 400-500 rpm, aireación de 0.5-1.0 vvm, y pH de 5.4.El hongo se desarrolló mejor utilizando la combinación de impulsores Rushton-Maxflo a 400 rpm y 1.0 vvm (F = 10.324, p £ 0.0123 (DMS=0.585, obteniendo una concentración de 1.2x109 blastosporas/ml, 2.2 g/l de biomasa y 2.48 g/l de consumo de sustrato (Y x/s=0.89. Las condiciones de mezclado y los parámetros obtenidos pueden ser aplicados en otros fermentadores para optimizar la producción de blastosporas de B. bassiana en la elaboración experimental de bioinsecticidas.Palabras clave: fermentación líquida, aireación, agitación, hongo entomopatógeno.Abstract: In this work three fermenters were characterized: New Brunswick M-19 of 14 liters, Biocontroller Applikon 1035 of 7 liters and New Brunswick Bioflo III of 7 liters, determining the volumetric coefficient of oxygen transfer (KLa, gas retention (GH and the mixing time (tM. The fermenter New Brunswick Bioflo III

  5. Production of extracellular proteolytic enzymes by Beauveria bassiana

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    Józefa Chrzanowska

    2014-08-01

    Full Text Available The production of proteolytic enzymes by two strains of Beauveria bassiana 278, B. bassiana 446 and one strain of Ascosphera apis 496 was analysed. It was demonstrated that the strain of B. bassiana 278 proved to be the best producer of basic and acid proteases. The influence of different environmental factors such as nitrogen and carbon sources on the production of extracellular hydrolytic enzymes was assessed. In addition the acid protease from B. bassiana was partially characterized.

  6. Beauveria bassiana: quercetinase production and genetic diversity

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    Eula Maria de M. B Costa

    2011-03-01

    Full Text Available Beauveria bassiana genetic diversity and ability to synthesize quercetin 2,3-dioxygenase (quercetinase were analyzed. B. bassiana isolates, obtained from Brazilian soil samples, produced quercetinase after induction using 0.5 g/L quercetin. B. bassiana ATCC 7159 (29.6 nmol/mL/min and isolate IP 11 (27.5 nmol/ml/min showed the best performances and IP 3a (9.5 nmol/mL/min presented the lowest level of quercetinase activity in the culture supernatant. A high level of polymorphism was detected by random amplified polymorphic DNA (RAPD analysis. The use of internal-transcribed-spacer ribosomal region restriction fragment length polymorphism (ITS-RFLP did not reveal characteristic markers to differentiate isolates. However, the ITS1-5.8S-ITS2 region sequence analysis provided more information on polymorphism among the isolates, allowing them to be clustered by relative similarity into three large groups. Correlation was tested according to the Person's correlation. Data of our studies showed, that lower associations among groups, level of quercetinase production, or geographical origin could be observed. This study presents the production of a novel biocatalyst by B. bassiana and suggests the possible industrial application of this fungal species in large-scale biotechnological manufacture of quercetinase.

  7. Patogenicidade de isolados de Beauveria bassiana para ovos, larvas e ninfas ingurgitadas de Rhipicephalus sanguineus Pathogenicity of Beauveria bassiana isolates towards eggs, larvae and engorged nymphs of Rhipicephalus sanguineus

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    Nancy Prette

    2005-08-01

    Full Text Available O presente trabalho objetivou avaliar a ação dos isolados JAB 07, CB 7 e AM 9 do fungo Beauveria bassiana para ovos, larvas e ninfas ingurgitadas de Rhipicephalus sanguineus. Padronizou-se a concentração de 109 conídios mL-1 para cada isolado e por meio de diluições seriadas, obtiveram-se as suspensões com concentrações de 10(8 e 10(7con. mL-1. Preparou-se um bioensaio para cada fase do ciclo de vida do carrapato. Cada ensaio foi composto por 10 grupos tratamentos, formados por um isolado do fungo e uma concentração de conídios, e um grupo controle contendo apenas o veículo das suspensões. Os isolados do fungo, aplicados nas diferentes concentrações de esporos, causaram redução no percentual de eclosão de larvas oriundas de ovos infectados (0,7 a 12,1% de eclosão e no percentual de ecdise de larvas (4,7 a 33,7% de ecdise e ninfas (0 a 16,7% de ecdise. Não houve diferença entre os isolados (P>0,05 quanto à infecção de ovos, mas a aplicação de 109 con./mL de JAB 07 e AM 9 promoveu redução significativa da eclosão de larvas, em relação à concentração de 10(7con. mL-1. O isolado CB 7 foi o mais eficaz na redução do número de ecdises de larvas. Os isolados e as concentrações de conídios não diferiram (P>0,05 quanto à capacidade de redução do percentual de ecdise de ninfas, mas evidenciou-se intensa atividade patogênica do fungo.The aim of the present study was to determine the action of isolates JAB 07, CB 7 and AM 9 of the fungus Beauveria bassiana on eggs, larvae and engorged nymphs of Rhipicephalus sanguineus tick. A concentration of 109 conidia mL-1 was standardized for each isolate and suspensions containing 10(8 and 10(7 conidia mL-1 were obtained by serial dilution. A bioassay was prepared for each phase of the life cycle of the tick. Each assay comprised 10 treatment groups, consisting of one fungal isolate and one conidial concentration, and one control group containing only the vehicle of

  8. Beauveria bassiana: endophytic colonization and plant disease control.

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    Ownley, Bonnie H; Griffin, Mary R; Klingeman, William E; Gwinn, Kimberly D; Moulton, J Kevin; Pereira, Roberto M

    2008-07-01

    Seed application of Beauveria bassiana 11-98 resulted in endophytic colonization of tomato and cotton seedlings and protection against plant pathogenic Rhizoctonia solani and Pythium myriotylum. Both pathogens cause damping off of seedlings and root rot of older plants. The degree of disease control achieved depended upon the population density of B. bassiana conidia on seed. Using standard plating techniques onto selective medium, endophytic 11-98 was recovered from surface-sterilized roots, stems, and leaves of tomato, cotton, and snap bean seedlings grown from seed treated with B. bassiana 11-98. As the rate of conidia applied to seed increased, the proportion of plant tissues from which B. bassiana 11-98 was recovered increased. For rapid detection of B. bassiana 11-98 in cotton tissues, we developed new ITS primers that produce a PCR product for B. bassiana 11-98, but not for cotton. In cotton samples containing DNA from B. bassiana11-98, the fungus was detected at DNA ratios of 1:1000; B. bassiana 11-98 was detected also in seedlings grown from seed treated with B. bassiana 11-98. Using SEM, hyphae of B. bassiana11-98 were observed penetrating epithelial cells of cotton and ramifying through palisade parenchyma and mesophyll leaf tissues. B. bassiana11-98 induced systemic resistance in cotton against Xanthomonas axonopodis pv. malvacearum (bacterial blight). In parasitism assays, hyphae of B. bassiana 11-98 were observed coiling around hyphae of Pythium myriotylum. PMID:18442830

  9. Safety of Beauveria bassiana (Bals. Vuill. to Gallus domesticus L.

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    Jucelaine Haas-Costa

    2010-04-01

    Full Text Available Beauveria bassiana, an important insect pathogen was used to evaluate its effect on the feeding, behavior, histology of the digestory system and anatomy of Gallus domesticus. The fungus (strain Unioeste 4 was administrated orally treated with chicken food. Chicken feces were collected, and the birds observed for 28 days. Also, were evaluated the weight of the birds, as well as any sign of intoxication or pathological modification. Tissue samples were withdrawn to test lesions with the optic microscope. There was 100% of survival of the birds, and no behavior alteration or external lesion was found. The test group presented the highest weight gain (1383.9±54.4g. Viable conidia were observed in the feces until 24 h after feeding the fungus, indicating that there was no germination inside the digestory system. No tissue lesion was observed, providing overwhelming evidence for the safety of B. bassiana to non-target organism G. domesticus.Beauveria bassiana é um importe entomopatógeno, sendo avaliado seu efeito sobre a alimentação, comportamento e histologia do sistema digestório de Gallus domesticus. O fungo (isolado Unioeste 4 foi administrado oralmente às aves, misturado à ração. Estas foram observadas por 28 dias e as fezes coletadas diariamente para análise. Também foi avaliado o peso das aves, bem como sinais de intoxicação ou modificação patológica. Amostras teciduais foram retiradas para verificar lesões com o microscópico óptico. Houve sobrevivência de 100% das aves avaliadas e nenhuma alteração comportamental ou lesão externa durante o experimento. O grupo teste apresentou o maior ganho de peso (1383,9±54,4g, sendo mais acentuado a partir da segunda semana. Observou-se a presença de conídios viáveis nas fezes somente até 24 horas após a ingestão do fungo, indicando que não houve germinação nas aves. Nenhuma lesão tecidual foi verificada, de forma que B. bassiana mostrou-se seguro para o organismo n

  10. 40 CFR 180.1205 - Beauveria bassiana ATCC #74040; exemption from the requirements of a tolerance.

    Science.gov (United States)

    2010-07-01

    ... ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) PESTICIDE PROGRAMS TOLERANCES AND EXEMPTIONS FOR PESTICIDE CHEMICAL... the insecticide Beauveria bassiana (ATCC #74040) in or on all food commodities when applied or used...

  11. Determinação da CL90 e TL90 do isolado IBCB66 de Beauveria bassiana (Ascomycetes: Clavicipitaceae para o controle de Rhipicephalus (Boophilus microplus (Acari: Ixodidae Determination of LC 90 and LT 90 of IBCB66 Beauveria bassiana (Ascomycetes: Clavicipitaceae isolate for Rhipicephalus (Boophilus microplus (Acari: Ixodidae control

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    Barci Leila A. G.

    2009-12-01

    Full Text Available O presente trabalho teve por objetivo avaliar a patogenicidade e a virulência do isolado IBCB66 de Beauveria bassiana para larvas de Rhipicephalus (Boophilus microplus. O isolado IBCB66 foi utilizado como padrão, com a finalidade de determinar a CL50 (Concentração Letal, CL90, TL50 (Tempo Letal e TL90. O isolado IBCB66 foi testado em seis concentrações diferentes (5 × 10(6, 10(7, 5 × 10(7, 10(8, 5 × 10(8 e 10(9 para determinar a porcentagem de mortalidade. A mortalidade total de larvas foi observada 18 dias após o início do teste no grupo tratado com 5 × 10(9 conídios.mL-1. A análise de Probit dos dados consignados apontou a CL50 e CL90 concentrações de 3 × 10(7 e 5 × 10(8 conídios.mL-1 e para TL50 e TL90, foram 10 e 16 dias, respectivamente.The objective of this research was to evaluate the pathogenicity and the virulence of the IBCB66 isolate of Beauveria bassiana on infected larvae of Rhipicephalus (Boophilus microplus. The IBCB66 fungus strain was used as standard isolates of B. bassiana against R. (B. microplus larvae. The larval bioassay tests using the IBCB66 isolate were carried out to determine the (Lethal Concentration LC50, LC90, (Lethal Time LT50 and LT90. The IBCB66 fungus strain was tested at six different concentrations (5 × 10(6, 10(7, 5 × 10(7, 10(8, 5 × 10(8 and 10(9 conidia.mL-1 to determine the percentage of larval mortality. In addition, a Probit analysis was also performed. Total larval mortality was observed eighteen days after the beginning of the test in the group treated with 5 × 10(9 conidia.mL-1. The LC50 and LC90 were 3 × 10(7 and 5 × 10(8 respectively and the LT50 and LT90 were 10 and 16 days.

  12. Entomopathogenic fungus \\kur{Beauveria bassiana }- biological characteristics

    OpenAIRE

    PAVLÍČEK, Pavel

    2012-01-01

    This literature compilation focuses on current knowledge in the biological characteristics of the entomopathogenic fungi Beauveria bassiana, with emphasis on the influence of abiotic factors on the development and its effectiveness in selected species of harmful insects. Knowledge of abiotic factors is important for a successful outcome to protect plants against harmful agents this entomopathogen. There are also set out the specifics, benefits and examples of using the aforementioned entomopa...

  13. Bestrijding van de slawortelboorder Hepialus lupulinus met de insectenpathogene schimmel Beauveria bassiana : praktijkproeven pioenroos 2004 - 2005

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    Boertjes, B.C.

    2005-01-01

    Uit het onderzoek kan geconcludeerd worden dat Beauveria bassiana een goede bestrijder is van de slawortelboorder Hepialus lupulinus L. Door de schimmel aangetaste rupsen zijn makkelijk aan te treffen in en buiten het behandelde perceel. Doordat in deze proeven Beauveria bassiana ook werd aangetroff

  14. Plant extracts on Hypothenemus hampei (Coleoptera: Curculionidae and Beauveria bassianaExtratos vegetais sobre Hypothenemus hampei (Coleoptera: Curculionidae e Beauveria bassiana

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    Janaina Zorzetti

    2012-12-01

    (moringa e Tephrosia purpurea (tephrosia (sementes, folhas e raízes e Melia azedarach,(cinamomo Nerium oleander, (espirradeira Azadirachta indica (nim (apenas folhas, na mortalidade e repelência de H. hampei, e sua compatibilidade com Beauveria bassiana, importante inimigo natural desta praga. Para avaliar a mortalidade, ofereceram-se aos adultos, folhas de cafeeiro (Coffea arabica L tratadas por imersão em solução de endosulfan e em extratos vegetais na concentração de 10%. A repelência foi avaliada em testes com e sem chance de escolha, entre frutos de cafeeiro tratados e não tratados. A compatibilidade entre os extratos e Beauveria bassiana (CG 452 foi analisada quantificando a germinação, unidades formadoras de colônias, crescimento vegetativo e produção/produtividade de conídios. As maiores mortalidades foram observadas quando folhas foram tratadas com o extrato etanólico das de T. purpurea (folhas que não diferiu do endosulfan, e de extrato aquoso e etanólico da semente de M. oleifera. Em testes com chance de escolha, todos os extratos etanólicos possuíram ação repelente, sendo que a maior repelência foi causada por M. oleifera (raiz e T. purpurea (semente. Os extratos aquosos de folhas e semente de M. oleifera e de folhas de N. oleander apresentaram a maior repelência. Nos ensaios sem chance de escolha o maior nível de repelência foi para frutos de cafeeiro tratados com A. indica (folhas. O extrato etanólico de folhas de M. oleifera afetou negativamente a germinação. Estes estudos mostraram o potencial desses extratos para uso a campo como uma alternativa ao controle químico, sendo também seletivos para B. bassiana.

  15. Multiple heavy metal removal using an entomopathogenic fungi Beauveria bassiana.

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    Gola, Deepak; Dey, Priyadarshini; Bhattacharya, Arghya; Mishra, Abhishek; Malik, Anushree; Namburath, Maneesh; Ahammad, Shaikh Ziauddin

    2016-10-01

    Towards the development of a potential remediation technology for multiple heavy metals [Zn(II), Cu(II), Cd(II), Cr(VI) and Ni(II)] from contaminated water, present study examined the growth kinetics and heavy metal removal ability of Beauveria bassiana in individual and multi metals. The specific growth rate of B. bassiana varied from 0.025h(-1) to 0.039h(-1) in presence of individual/multi heavy metals. FTIR analysis indicated the involvement of different surface functional groups in biosorption of different metals, while cellular changes in fungus was reflected by various microscopic (SEM, AFM and TEM) analysis. TEM studies proved removal of heavy metals via sorption and accumulation processes, whereas AFM studies revealed increase in cell surface roughness in fungal cells exposed to heavy metals. Present study delivers first report on the mechanism of bioremediation of heavy metals when present individually as well as multi metal mixture by entomopathogenic fungi.

  16. Spore production of Beauveria bassiana from agro-industrial residues

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    Herta Stutz Dalla Santa; Osmar Roberto Dalla Santa; Débora Brand; Luciana Porto de Souza Vandenberghe; Carlos Ricardo Soccol

    2005-01-01

    The purpose of this work was to produce Beauveria bassiana by Solid-State Fermentation using agro-industrial residues and optimizing the cultivation conditions. Refused potatoes, coffee husks and sugar-cane bagasse were tested. The blend of refused potatoes and sugar-cane bagasse (60-40%) with particle size in the range of 0.8-2 mm was used in the fermentation experiments. In Erlenmeyer flasks the best spore production was achieved with the following conditions: incubation temperature 26º C; ...

  17. Genetic diversity among brazilian isolates of beauveria bassiana: comparisons with non-brazilian isolates and other beauveria species

    Science.gov (United States)

    Fernandes, E.K.K.; Moraes, A.M.L.; Pacheco, R.S.; Rangel, D.E.N.; Miller, M.P.; Bittencourt, V.R.E.P.; Roberts, D.W.

    2009-01-01

    Aims: The genetic diversity of Beauveria bassiana was investigated by comparing isolates of this species to each other (49 from different geographical regions of Brazil and 4 from USA) and to other Beauveria spp. Methods and Results: The isolates were examined by multilocus enzyme electrophoresis (MLEE), amplified fragment length polymorphism (AFLP), and rDNA sequencing. MLEE and AFLP revealed considerable genetic variability among B. bassiana isolates. Several isolates from South and Southeast Brazil had high similarity coefficients, providing evidence of at least one population with clonal structure. There were clear genomic differences between most Brazilian and USA B. bassiana isolates. A Mantel test using data generated by AFLP provided evidence that greater geographical distances were associated with higher genetic distances. AFLP and rDNA sequencing demonstrated notable genotypic variation between B. bassiana and other Beauveria spp. Conclusion: Geographical distance between populations apparently is an important factor influencing genotypic variability among B. bassiana populations in Brazil. Significance and Impact of the Study: This study characterized many B. bassiana isolates. The results indicate that certain Brazilian isolates are considerably different from others and possibly should be regarded as separate species from B. bassiana sensu latu. The information on genetic variation among the Brazilian isolates, therefore, will be important to comprehending the population structure of B. bassiana in Brazil. ?? 2009 The Society for Applied Microbiology.

  18. Development of Metarhizium anisopliae and Beauveria bassiana formulations for control of malaria mosquito larvae

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    Bukhari, S.T.; Takken, W.; Koenraadt, C.J.M.

    2011-01-01

    Background The entomopathogenic fungi Metarhizium anisopliae and Beauveria bassiana have demonstrated effectiveness against anopheline larvae in the laboratory. However, utilising these fungi for the control of anopheline larvae under field conditions, relies on development of effective means of app

  19. Natural variation in virulence of the entomopathogenic fungus Beauveria bassiana against malaria mosquitoes

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    Valero Jimenez, C.A.; Debets, A.J.M.; Kan, van J.A.; Schoustra, S.E.; Takken, W.; Zwaan, B.J.; Koenraadt, C.J.M.

    2014-01-01

    Background Insecticide resistance is greatly hampering current efforts to control malaria and therefore alternative methods are needed. Entomopathogenic fungi have been proposed as an alternative with a special focus on the cosmopolitan species Beauveria bassiana. However, few studies have analysed

  20. Beauveria bassiana and Metarhizium anisopliae endophytically colonize cassava roots following soil drench inoculation

    Science.gov (United States)

    The fungal entomopathogens Beauveria bassiana and Metarhizium anisopliae were investigated to determine if endophytic colonization could be achieved in cassava. An inoculation method based on drenching the soil around cassava stems using conidial suspensions resulted in endophytic colonization of ca...

  1. The use of aggregation pheromone to enhance dissemination of Beauveria bassiana for the control of the banana weevil in Uganda

    NARCIS (Netherlands)

    Tinzaara, W.; Gold, C.S.; Dicke, M.; Huis, van A.; Nankinga, C.M.; Kagezi, G.H.; Ragama, P.E.

    2007-01-01

    Candidate strains of Beauveria bassiana were identified for use in integrated pest management of the banana weevil Cosmopolites sordidus. Horizontal field transmission of B. bassiana between banana weevils using different delivery systems, including aggregation pheromones, was investigated. We obser

  2. Compatibility of the insect pathogenic fungus Beauveria bassiana with neem against sweetpotato whitefly, Bemisia tabaci, on eggplant

    Science.gov (United States)

    A study on the compatibility of the entomopathogenic fungus Beauveria bassiana (Balsamo) Vuillemin (Ascomycota: Hypocreales) with neem was conducted against sweetpotato whitefly, Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae), on eggplant. Initially, three concentrations of B. bassiana (106, 1...

  3. Desiccation increases the efficacy of Beauveria bassiana for stored grain pest insects

    Science.gov (United States)

    The effect of desiccation stress on the efficacy of Beauveria bassiana for stored-product insects was investigated in laboratory bioassays. The mortality of B. bassiana-treated Plodia interpunctella larvae was greater at a vapor pressure deficit (VPD) of 2.42 kPa or 1.87 kPa than at 1.06 kPa. Moist...

  4. Laboratory Study on Biological Control of Ticks (Acari: Ixodidae by Entomopathogenic Indigenous Fungi (Beauveria bassiana

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    M Abdigoudarzi

    2009-12-01

    Full Text Available Background: Chemical control method using different acaricides as spray, dipping solution or pour-on is routinely used for controlling ticks. Biological control agents are favorable due to their safety for animals and environment. Entomopathogenic fungi such as Beauveria bassiana are well known for controlling ticks. In this study, two Iranian indigenous strains of B. bassiana (B. bassiana 5197 and B. bassiana Evin were selected and grown on specific me­dia. The pathogenic effects of these strains were evaluated on adult stages of two Iranian Ixodidae members (H. anatolicum anatolicum Koch 1844, and H. marginatum Koch 1844 by dipping method.Methods: Two Iranian strains of Beauveria bassiana (Beauveria bassiana 5197 and Beauveria bassiana Evin were selected and were grown successfully on specific media. The pathogenic effects of these strains were evaluated on adult stages of Iranian Ixodidae members such as, Hyalomma anatolicum anatolicum and H. marginatum by dipping method (these ticks were grown up at laboratory conditions during 2002 up to 2003 and still it is continued .Results: There was no effect of strain 5197 on mortality or fecundity rates for ticks. There was acute phase sign of paralysis in test group after dipping ticks in suspension made from Evin strain of B. bassiana. In addition, the test groups were totally died after four months, but the control groups survived for six months.Conclusion: High concentration of fungal spores is needed for inducing fungal infection. Additional study using different strains and fungi on Iranian ticks is proposed. 

  5. Cordyceps bassiana and production of stromata in vitro showing Beauveria anamorph in Korea

    Science.gov (United States)

    A Cordyceps species was found with Beauveria anamorph state on larval insect cadavers on Obong Mountsin in Gangwon Pronvince, Republic of Korea. Cultures from discharged ascospores formed an anamorph identifiable as Beauveria bassiana. This teleomorph-anamorph connection was also confirmed by the in...

  6. Spore production of Beauveria bassiana from agro-industrial residues

    Directory of Open Access Journals (Sweden)

    Herta Stutz Dalla Santa

    2005-06-01

    Full Text Available The purpose of this work was to produce Beauveria bassiana by Solid-State Fermentation using agro-industrial residues and optimizing the cultivation conditions. Refused potatoes, coffee husks and sugar-cane bagasse were tested. The blend of refused potatoes and sugar-cane bagasse (60-40% with particle size in the range of 0.8-2 mm was used in the fermentation experiments. In Erlenmeyer flasks the best spore production was achieved with the following conditions: incubation temperature 26º C; initial pH 6.0; inoculum concentration 10(7 spores.g-1.dw and initial moisture 75%. In the column type reactor using forced aeration under the optimized conditions, the maximum production (1.07x10(10spores.g-1.dw was obtained at the 10th day of fermentation. The respirometric analyses of the fermentation showed a strong correlation between fungal growth and spore production.O objetivo deste trabalho foi produzir Beauveria bassiana por fermentação no estado sólido em resíduos agro-industriais e otimizar as condições de cultivo. Batata-refugo, polpa de café e bagaço de cana de açúcar foram testados. A mistura de batata-refugo e de bagaço de cana de açúcar (60:40%, com granulometria de 2 a 0,8 mm foi escolhida como melhor substrato/suporte. Em frascos de Erlenmeyer a produção de esporos foi maior com as seguintes condições: pH 6,0; temperatura de incubação de 26º C; taxa de inóculo de 10(7 esporos.g-1 de matéria seca; e umidade inicial de 75%. Em bioreator do tipo coluna com aeração forçada, as condições otimizadas possibilitaram uma produção máxima de esporos no 10º dia de fermentação, obtendo-se 1,07x10(10 esporos.g-1 de matéria seca. A análise respirométrica desta fermentação permitiu correlacionar o desenvolvimento do fungo com a produção de esporos.

  7. Existence of entomopathogen fungi, Beauveria bassiana as an endophyte in cacao seedlings

    Directory of Open Access Journals (Sweden)

    Endang Sulistyowati

    2015-12-01

    Full Text Available Beauveria bassiana is one of the entomopathogen fungi which is known as biological control agent of cocoa pod borer and cocoa mirids (Helopeltis spp.. Because of its effectiveness in the fields is still not consistent, so we conduct a research with the objective to know the possibility of Beauveria bassiana to be established as a endophyte. Various fungal entomopathogens have already been reported as endophytes and the various methods used to inoculate the plants with B. bassiana were partially effective. The research has been conducted in laboratory of Plant Protection, Indonesian Coffee and Cocoa Research Institute by inoculating of cocoa seeds and cocoa nursery with B. bassiana suspension.  The trial was arranged  by randomized complete block design with a factorial arrangement. The factor were spore concentration of B. bassiana (0; 2; and 4 g/ 10 l and cocoa varieties (family of ICS 60, TSH858, and hybrid. The trial were use  four replications. The results showed that the fungal entomopathogen B. bassiana was established as an endophyte in cocoa seedling, both from cocoa seeds and nursery application. Percentage of existence of B. bassiana colonies as endophytes one month after seeds application were ICS 60 amounted to 93.3 % both on concentration treatments, while the families of TSH 858 by 80 % and 86.67 % respectively in 2 g and 4 g per 10 l of B. bassiana spores concentration treament.. The lowest percentage was in hybrids, which amounted to 66.67% and 50%. B. bassiana colonies was exixtence as an endophyte in culture from root, stem and leaves of cocoa seedling up to 5 months post inoculation. While the application on nursery by soil drenshing, leaf spraying, and stem injection , it was known that B. bassiana colonies were found in the tissues of leaves, stems, and roots until two months after application. Colonies of B. bassiana as endophytes still exsist until six weeks after nursery was planted in the field. 

  8. Production of conidia of Beauveria bassiana in solid-state culture: current status and future perspectives.

    Science.gov (United States)

    Lopez-Perez, M; Rodriguez-Gomez, D; Loera, O

    2015-01-01

    Beauveria bassiana is an important entomopathogenic fungus widely commercialized in the world. Recent progress and achievements on conidia production have focused on a yield goal of 10(9) to 10(10) conidia per gram of dry substrate. Due to cost-competitive perspectives, these yields should be associated with better production rates or productivities. This study presents a review of relevant studies of B. bassiana conidia production on solid-state cultures and the parameters that should be taken into account to maintain constant quality in the product to be commercialized. Conditions for maximizing production and infectivity of B. bassiana conidia are also analysed. PMID:24494702

  9. Sensitivity of the Entomogenous Fungus Beauveria bassiana to Selected Plant Growth Regulators and Spray Additives

    Science.gov (United States)

    Storey, Greggory K.; Gardner, Wayne A.

    1986-01-01

    Mefluidide was the only one of four plant growth regulators that caused little to no significant inhibition of in vitro germination and growth of the entomogenous fungus Beauveria bassiana. Silaid, paclobutrazol, and flurprimidol significantly inhibited germination and growth. Mortality of fall armyworm, Spodoptera frugiperda, resulting from B. bassiana was significantly reduced when larvae were exposed to conidia plus soil treated with paclobutrazol. Larval mortality resulting from conidia plus soil treated with mefluidide did not differ significantly from mortality resulting from untreated conidia. Triton CS-7 was the only one of eight spray adjuvants that significantly inhibited germination of B. bassiana conidia. PMID:16347095

  10. Improved shelf life of dried Beauveria bassiana blastospores using convective drying and active packaging processes

    Science.gov (United States)

    The yeast form (blastospore) of the dimorphic insect-pathogenic fungus Beauveria bassiana can be rapidly produced using liquid fermentation methods but is generally unable to survive rapid dehydration processes or storage under non-refrigerated conditions. In this study, we evaluated the influence o...

  11. Anopheline and culicine mosquitoes are not repelled by surfaces treated with the entomopathogenic fungi Metarhizium anisopliae and Beauveria bassiana

    NARCIS (Netherlands)

    Mnyone, L.L.; Koenraadt, C.J.M.; Lyimo, I.N.; Mpingwa, M.W.; Takken, W.; Russell, T.L.

    2010-01-01

    Background - Entomopathogenic fungi, Metarhizium anisopliae and Beauveria bassiana, are promising bio-pesticides for application against adult malaria mosquito vectors. An understanding of the behavioural responses of mosquitoes towards these fungi is necessary to guide development of fungi beyond t

  12. Survival of anopheline eggs and their susceptibility to infection with Metarhizium anisopliae and Beauveria bassiana under laboratory conditions

    NARCIS (Netherlands)

    Luz, C.; Mnyone, L.L.; Russell, T.L.

    2011-01-01

    The viability of Anopheles gambiae sensu stricto and Anopheles arabiensis (Diptera: Culicidae) eggs over time and the ovicidal activity of Beauveria bassiana (Ascomycota: Cordycipitaceae) and Metarhizium anisopliae (Ascomycota: Clavicipitaceae) were investigated. Eggs were incubated in soil or leaf

  13. Isolation and Assessment of Stability of Six Formulations of Entomopathogenic Beauveria bassiana.

    Science.gov (United States)

    Mwamburi, Lizzy A

    2016-01-01

    Beauveria bassiana is the most widely studied and exploited entomopathogen. The development of a suitable formulation for B. bassiana is a critical component in aiding the entomopathogen germinate and infect the host. In addition to being economical to produce, having high residual activity, it is also important that the formulation is easy to handle, stable during storage, and convenient to mix and apply and be consistently effective in controlling the target pest.In this chapter we describe preparation of experimental formulations of conidia of B. bassiana. The formulations are prepared with barley, rice, wheat bran, clay, kaolin, and peat. The protocol for assessing the stability of the formulations of B. bassiana is also described. PMID:27565494

  14. Discovery and demonstration of the teleomorph of Beauve-ria bassiana (Bals.) Vuill. , an important entomogenous fun-gus

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    A Cordyceps specimen was collected in Anhui, China, a strain of Beauveria bassiana, an important entomopathogenic fungus for biological pest control, was isolated and their relationship was demonstrated by microcycle conidiation. The teleomorph is an undescribed species and is named Cordyceps bassiana.

  15. Screening of liquid media and fermentation of an endophytic Beauveria bassiana strain in a bioreactor

    OpenAIRE

    Lohse, Rieke; Jakobs-Schönwandt, Desiree; Patel, Anant V

    2014-01-01

    A novel approach for biological control of insect pests could be the use of the endophytic entomopathogenic Beauveria bassiana isolate ATP-02. For the utilization of the endophyte as a commercial biocontrol agent, the fungus has to be mass-produced. B. bassiana was raised in shake flask cultures to produce high concentrations of total spores (TS), which include blastospores (BS) and submerged conidiospores (SCS). The highest concentration of 1.33×109 TS/mL and the highest yield of 5.32×1010 T...

  16. rRNA sequence comparison of Beauveria bassiana, Tolypocladium cylindrosporum, and Tolypocladium extinguens.

    Science.gov (United States)

    Rakotonirainy, M S; Dutertre, M; Brygoo, Y; Riba, G

    1991-01-01

    Five strains of Tolypocladium cylindrosporum, one strain of Tolypocladium extinguens, and nine strains of Beauveria bassiana were analyzed using a rapid rRNA sequencing technique. The sequences of two highly variable domains (D1 and D2) located at the 5' end of the 28S-like rRNA molecule were determined. The phylogenetic tree computed from the absolute number of nucleotide differences shows the separation between the genus Beauveria and the genus Tolypocladium and points out that T. cylindrosporum and T. extinguens probably do not belong to the same genus.

  17. PATOGENICIDADE DE BEAUVERIA BASSIANA (BALS.) VUILL. AO ÁCARO Tetranychus urticae KOCH PATHOGENICITY OF BEAUVERIA BASSIANA (BALS.) VUILL. AGAINST Tetranychus urticae KOCH

    OpenAIRE

    Marco Antonio Tamai; Sérgio Batista Alves; Pedro Janeiro Neves

    1999-01-01

    O estudo da patogenicidade de Beauveria bassiana (Bals.) Vuill. ao ácaro Tetranychus urticae Koch foi desenvolvido a 25 ± 2°C, 70 ± 5% UR e 12 horas de fotofase, utilizando-se fêmeas recém-emergidas. Os ácaros foram mantidos durante seis dias em discos de folha de Canavalia ensiformis (L.) DC. inoculadas com B. bassiana, isolado 447, nas concentrações: 5x106, 1x107, 5x107, 1x108, 5x108 e 1x109 conídios/ml. Este isolado mostrou-se patogênico a T. urticae, apresentando aumento nos valores das m...

  18. Use of Beauveria bassiana and Metarhizium anisopliae for fruit fly control: a novel approach

    International Nuclear Information System (INIS)

    The potential of two species of entomopathogenic fungi, Beauveria bassiana (Bals.) and Metarhizium anisopliae (Met.) Sorokin, as practical fruit fly biocontrol agents is studied. These natural inhabitants of soil are found infecting a wide range of insect species that spend at least one stage of their life cycle in the soil. Sterile flies are used as vectors of the infection. A summary of results from different laboratory and field cage experiments is presented. (MAC)

  19. Optimization of Solid-State Fermentation for Improved Conidia Production of Beauveria bassiana as a Mycoinsecticide

    OpenAIRE

    Pham, Tuan Anh; Kim, Jeong Jun; Kim, Keun

    2010-01-01

    The production of conidia of entomopathogenic Beauveria bassiana by solid-state fermentation was studied for the development of a biocontrol agent against aphid Myzus persicae. The optimal conditions for conidia production on polished white rice were 40% moisture content, 25℃ culture temperature, 2-day-old seeding culture grown in 3% corn meal, 2% rice bran, 2% corn steep powder medium, initial conidia concentration of 107 conidia/g in the wet rice, 10% inoculum size, and use of a polyethylen...

  20. Use of Beauveria bassiana and Metarhizium anisopliae for fruit fly control: a novel approach

    Energy Technology Data Exchange (ETDEWEB)

    Toledo, Jorge; Liedo, Pablo, E-mail: jtoledo@ecosur.m [El Colegio de la Frontera Sur, Chiapas (Mexico). Dept. de Entomologia Tropical; Flores, Salvador; Montoya, Pablo [Secretaria de Agricultura, Ganaderia, Desarrollo Rural, Pesca y Alimentacion (SAGARPA), Chiapas (Mexico). Subdireccion de Desarrollo de Metodos; Campos, Sergio E.; Villasenor, Antonio [Secretaria de Agricultura, Ganaderia, Desarrollo Rural, Pesca y Alimentacion (SAGARPA), Chiapas (Mexico). Programa Moscamed. Direccion de Operaciones de Campo

    2006-07-01

    The potential of two species of entomopathogenic fungi, Beauveria bassiana (Bals.) and Metarhizium anisopliae (Met.) Sorokin, as practical fruit fly biocontrol agents is studied. These natural inhabitants of soil are found infecting a wide range of insect species that spend at least one stage of their life cycle in the soil. Sterile flies are used as vectors of the infection. A summary of results from different laboratory and field cage experiments is presented. (MAC)

  1. Efficacy of Molasses and Beauveria bassiana (Balsamo) Vuill on the Control of Cosmopolites sorditus Germar, 1824

    OpenAIRE

    Flávia Queiroz de Oliveira; José Bruno Malaquias; Luiz Leonardo Ferreira; Thiago de Souza Ribeiro; Alexandre Igor de Azevedo Pereira

    2010-01-01

    This research sought to study the compatibility between the molasses and the entomopathogenic fungi Beauveria bassiana (Balsamo) Vuill on the control of Cosmopolites sorditus Germar, 1824. The study was conducted on the Site Pitiá in a banana plantation of approximately 250 m2, located in the municipality of Areia, state of Paraiba, Brazil. The results showed that the constancy of C. sorditus was significantly higher in traps consisting of molasses+fungi (P

  2. Selection of Beauveria bassiana and Metarhizium anisopliae Isolates to Control Triatoma infestans

    Directory of Open Access Journals (Sweden)

    Luz Christian

    1998-01-01

    Full Text Available Twenty three isolates of Beauveria bassiana and 13 isolates of Metarhizium anisopliae were tested on third instar nymphs of Triatoma infestans, a serious vector of Chagas disease. Pathogenicity tests at saturated humidity showed that this insect is very susceptible to fungal infection. At lower relative humidity (50%, conditions expected in the vector microhabitat, virulence was significantly different among isolates. Cumulative mortality 15 days after treatment varied from 17.5 to 97.5%, and estimates of 50% survival time varied from 6 to 11 days. Maintaining lower relative humidity, four B. bassiana and two M. anisopliae isolates were selected for analysis of virulence at different conidial concentrations and temperatures. Lethal concentrations sufficient to kill 50% of insects (LC50 varied from 7.1x105 to 4.3x106 conidia/ml, for a B. bassiana isolate (CG 14 and a M. anisopliae isolate (CG 491 respectively. Most isolates, particularly B. bassiana isolates CG 24 and CG 306, proved to be more virulent at 25 and 30°C, compared to 15 and 20°C. The differential virulence at 50% humidity observed among some B. bassiana isolates was not correlated to phenetic groups in cluster analysis of RAPD markers. In fact, the B. bassiana isolates analyzed presented a high homogeneity (> 73% similarity.

  3. Polymorphism of Beauveria bassiana (Deuteromycota: Hyphomycetes) strains isolated from Ixodes ricinus (Acari: Ixodidae) in Moldova.

    Science.gov (United States)

    Mitina, G V; Tokarev, Y S; Movila, A A; Yli-Mattila, T

    2011-03-01

    Polymorphism of 10 Beauveria bassiana strains, isolated from Ixodes ricinus in Moldova, was evaluated using traditional (morphological and cultural properties) and molecular (RAPD patterns and ITS sequences) methods. The isolates differed greatly in morphological and cultural features, such as color, consistence, and growth rate. Four RAPD-PCR markers were used to evaluate genetic diversity of the strains. Phylogenetic neighbor-joining analysis of RAPD patterns divided strains into 3 major clades. The ITS sequences of 8 strains were identical to those of known B. bassiana strains. Two subsets (1 and 2) different by one nucleotide change were found in the ITS1 region. One strain of subset 1 was different from known B. bassiana strains by possessing 2 point mutations in the ITS region. RAPD-based clustering correlated to ITS sequence and colony morphology-based grouping of the strains.

  4. Soil application of Beauveria bassiana GHA against apple sawfly, Hoplocampa testudinea (Hymenoptera: Tenthredinidae)

    DEFF Research Database (Denmark)

    Świergiel, Weronika; Meyling, Nicolai Vitt; Porcel, Mario;

    2016-01-01

    Low impact alternatives to synthetic insecticides for the control of apple sawfly (Hoplocampa testudinea Klug) are scarce encumbering pest management in organic apple orchards. We investigated the soil persistence and field efficacy of the entomopathogenic fungus Beauveria bassiana (Balsamo......) Vuillemin (BotaniGard) against apple sawfly under common organic orchard practices. We also assessed the efficacy of B. bassiana GHA and Metarhizium brunneum Petch (indigenous strain) against sawfly in the laboratory. Larvae treated with either fungus in the laboratory died faster than control larvae...... and displayed 49.4%-68.4% mycosis. In the field, B. bassiana density remained high in the week after application, during larval descent to the soil. Fungal density decreased to 25% at 49 d after application and to 0.4% after 55 weeks. Molecular markers revealed that the majority of fungal isolates recovered...

  5. PATOGENITAS CENDAWAN BEAUVERIA BASSIANA TERHADAP LARVA NYAMUK AEDES AEGYPTI DAN CULEX PIPIENS QUINQUEFASCIATUS DI LABORATORIUM

    Directory of Open Access Journals (Sweden)

    Amrul Munif

    2012-09-01

    Full Text Available The capability of Beauveria bassiana fungus to kill mosquito larvae was challenged with Aedes aegypti and Culex pipiens quinquefasciatus in a study conducted at the entomology laboratory of the Health Ecology Research Centre. Cx. p. quinquefeasciatus was more sensitive compared to Ae. aegypti to the B. bassiana strain from Sukamandi (West Java, which is probably due to the mosquitoes behaviour and conidiospore larvacidal effect. Conidia dust application, with a dosage of 2.2 mglliter, to water surface, within 48 hours was able to kill almost all the Cx. p. quinquefasciatus. However a dosage of 4 mglliter was required to kill all the Ae.aegypti. And a dosage of 1.3 mg conidiospore I liter is able to kill 50% Cx. p. quinquefasciatus. It seems that B. bassiana has greater capability to eradicate Cx. p. quinquefasciatus compared to Ae. aegypti

  6. Selectivity of Beauveria bassiana and Metarrhizium anisopliae to Trichogramma pretiosum Riley (Hymenoptera: Trichogrammatidae); Seletividade de Beauveria bassiana e Metarhizium anisopliae a Trichogramma pretiosum Riley (Hymenoptera: Trichogrammatidae)

    Energy Technology Data Exchange (ETDEWEB)

    Potrich, Michele; Silva, Everton L. da; Neves, Pedro M.O.J. [Universidade Estadual de Londrina (UEL), PR (Brazil). Centro de Ciencias Agrarias], e-mail: profmichele@gmail.com, e-mail: evertonloz@gmail.com, e-mail: pedroneves@uel.br; Alves, Luis F.A.; Daros, Alaxsandra [Universidade do Oeste do Parana (UNIOESTE), Cascavel, PR (Brazil). Centro de Ciencias Biologicas e da Saude. Lab. de Zoologia de Invertebrados], e-mail: lfaalves@unioeste.br; Haas, Jucelaine; Pietrowski, Vanda [Universidade do Oeste do Parana (UNIOESTE), Marechal Candido Rondon, PR (Brazil). Centro de Ciencias Agrarias], e-mail: jubarth@gmail.com, e-mail: vandapietrowski@gmail.com

    2009-07-01

    Trichogramma pretiosum Riley and the entomopathogenic fungi Beauveria bassiana and Metarhizium anisopliae are efficient biological control agents and are thought to be used jointly. In here, we investigated if these entomopathogens could have any side-effects on T. pretiosum. Therefore, 1 x 8 cards containing sterilized eggs of Anagasta kuehniella (Zeller) that were sprayed with 0.2 ml of B. bassiana or M. anisopliae (1.0 X 10{sup 9} conidia/ml) were offered to a T. pretiosum female for 24h (30 cards/fungus = 30 replicates). Afterwards, females were isolated in glass tubes. The control group was sprayed with sterile distillated water + Tween 80 (0.01%). In addition, 60 cards with sterilized eggs of A. kuehniella were submitted to parasitism by females of T. pretiosum for 24h. Of these cards, 30 were sprayed with B. bassiana or M. anisopliae and 30 with distillated water + Tween 80 (0.01%), and observed daily until parasitoid emergence. Metarhizium anisopliae decreased parasitoid emergence and caused confirmed mortality. Therefore, field and semi-field experiments should be conducted for a final assessment of the side-effects of these entomopathogens on Trichogramma as a ways to develop a control strategy in which both can be used. (author)

  7. Compatibility between the entomopathogenic fungus Beauveria bassiana and insecticides used in coffee plantations Compatibilidade entre o fungo entomopatogênico Beauveria bassiana e inseticidas usados na cultura do cafeeiro

    Directory of Open Access Journals (Sweden)

    Carolina Natali de Oliveira

    2003-12-01

    controle, reduzindo assim a quantidade de inseticidas. O efeito fungitóxico in vitro das formulações inseticidas de Tiametoxan, Ciflutrin, Deltametrin, Alfacipermetrina, Triazofos, Clorpirifós, Fenpropatrin e Endosulfan em três concentrações (RC= recomendação média para campo, 0,5 ´ RC e 2 ´ RC, foi avaliado sobre a germinação dos conídios, crescimento vegetativo e produção de conídios no fungo Beauveria bassiana. Apenas cinco formulações inseticidas, na concentração RC, proporcionaram viabilidade dos conídios acima de 60%. A viabilidade deve ser considerada o parâmetro mais importante a ser avaliado por ser o passo inicial no processo de infecção. As formulações de Alfacipermetrina, Tiametoxan e Ciflutrin, nas menores concentrações, causaram a menor inibição da germinação, sem diferença em relação à testemunha. Analisando-se o crescimento vegetativo, observou-se que a formulação de Tiametoxan nas menores concentrações, não inibiu o crescimento radial, sendo o crescimento vegetativo nos demais tratamentos inferior à testemunha. A formulação de Tiametoxan também proporcionou a menor inibição na produção de conídios. Os inseticidas com formulações de Alfacipermetrina e Tiametoxan mostraram-se compatíveis com o fungo Beauveria bassiana (CG 425, importante agente natural de controle da broca do café Hypothenemus hampei e podem ser recomendados para MIP no cafeeiro

  8. The production and uses of Beauveria bassiana as a microbial insecticide.

    Science.gov (United States)

    Mascarin, Gabriel Moura; Jaronski, Stefan T

    2016-11-01

    Among invertebrate fungal pathogens, Beauveria bassiana has assumed a key role in management of numerous arthropod agricultural, veterinary and forestry pests. Beauveria is typically deployed in one or more inundative applications of large numbers of aerial conidia in dry or liquid formulations, in a chemical paradigm. Mass production is mainly practiced by solid-state fermentation to yield hydrophobic aerial conidia, which remain the principal active ingredient of mycoinsecticides. More robust and cost-effective fermentation and formulation downstream platforms are imperative for its overall commercialization by industry. Hence, where economics allow, submerged liquid fermentation provides alternative method to produce effective and stable propagules that can be easily formulated as dry stable preparations. Formulation also continues to be a bottleneck in the development of stable and effective commercial Beauveria-mycoinsecticides in many countries, although good commercial formulations do exist. Future research on improving fermentation and formulation technologies coupled with the selection of multi-stress tolerant and virulent strains is needed to catalyze the widespread acceptance and usefulness of this fungus as a cost-effective mycoinsecticide. The role of Beauveria as one tool among many in integrated pest management, rather than a stand-alone management approach, needs to be better developed across the range of crop systems. Here, we provide an overview of mass-production and formulation strategies, updated list of registered commercial products, major biocontrol programs and ecological aspects affecting the use of Beauveria as a mycoinsecticide. PMID:27628337

  9. The characteristics of keratomycosis by Beauveria bassiana and its successful treatment with antimycotic agents

    Directory of Open Access Journals (Sweden)

    Hiroko Sonoyama

    2008-10-01

    Full Text Available Hiroko Sonoyama1, Kaoru Araki-Sasaki1, Shigeyasu Kazama1, Tsutomu Kawasaki1, Hidenao Ideta1, Atsuko Sunada2, Seishi Asari2, Yoshitsugu Inoue3, Kozaburo Hayashi41Ideta Eye Hospital, Kumamoto, Kumamoto, Japan; 2Department of Laboratory for Clinical Investigation, Osaka University Hospital, Suita, Osaka, Japan; 3Division of Ophthalmology and Visual Science, Department of Medicine of Sensory and Motor Organ, Tottori University, Yonago, Tottori, Japan; 4Immunology and Virology Section Lab, Immunology, NEI, NIH, Bethesda, MD, USAAbstract: Clinical findings and treatment of keratomycosis caused by Beauveria bassiana, an entomopathogenic filamentous fungus, are described for an 80-year-old woman, who was referred to the hospital for ocular pain and redness on the 9th day after an ocular injury caused by the frame of her glasses. She had a long history of recurrent diabetic iritis and continuously used topical antibiotics and corticosteroids. At her first visit, a slit-lamp examination indicated a corneal ulcer confined within the superficial stromal layer, along with a slight infiltration and edema. Only a very few inflammatory cells were seen in the anterior chamber. Direct microscopic examination of corneal scrapings revealed septate fungal hyphae with zig-zag rachis and budding that was subsequently identified as B. bassiana by slide culture. Topical voriconazole with miconazole, pimaricin and oral itraconazole were effective and the lesion disappeared leaving only a mild scar at 2 months. The sensitivity of B. bassiana to various antimycotic agents was confirmed by broth microdilution, agar dilution with the Clinical Laboratory Standard Institute standard, and a disk method using topically applied concentrations. B. bassiana, which exhibits a characteristic appearance in smears and causes superficial keratomycosis, is sensitive to voriconazole with miconazole, pimaricin, and itraconazole.Keywords: Beauveria bassiana, keratomycosis, filamentous

  10. Evaluation of kinetic parameters of chitinases produced by Beauveria bassiana (Bals.) Vuill. /
    Avaliação de parâmetros cinéticos de quitinases produzidas por Beauveria bassiana (Bals.) Vuill.

    OpenAIRE

    Cristiane Mita; Vanessa Hitomi Sugahara; Jo I Wu; Pedro Manoel Oliveira Janeiro Neves; Dalva Tomoe Miyagui; Geni Varéa-Pereira; Danieli Cristina Sassá; Evelyn Kamogawa

    2008-01-01

    Entomopathogenic fungus Beauveria bassiana is currently used as a biocontrol agent for agricultural pests. The infection process involves extracellular enzymes such as proteases and chitinases that degrade the cuticle of the insects. The objective of this work was to evaluate kinetic parameters of pH, temperature, ionic concentration and time of reaction on chitinases activity. The fungus B. bassiana CG432 was cultivated on coffee berry borer Hypothenemus hampei (Ferrari) and the conidia grow...

  11. Effect of Storage Temperature on Beauveria bassiana (Bals. Vuill. Viability on Several Carriers

    Directory of Open Access Journals (Sweden)

    Sri Sukamto

    2006-05-01

    Full Text Available One of the entomopatogenous fungus types commonly observed and showed potency as myco-insectiside is Beauveria bassiana(Bals. Vuill.In order to support effectiveness and patogenous activity of B. bassiana, it is necessary to add a carrying agent that protects its spores from ultra violet ray. This study aims to investigate the effect of storage temperature on viability of B. Bassianaspores on the carrier material. The observation was carried out in the Laboratory of Plant Diseases, Indonesian Coffee and Cocoa Research Institute. The research was arranged in completely randomized design by three factors. The first factor was carrier (C, that consists of C1 = rice flour, C2 = maize flour and C3 = tapioca flour. The second factor was dosage (D, that consists of D1 = 1 g B. bassiana+ 1 g carrier; D2 = 1 g B. bassiana+ 5 g carrier and D3 = 1 g B. bassiana+ 10 g carrier. The third factor was temperature of the storage (T,that consists of T1 = 5oC; T2 = 23oC and T3 = 29oC. Viability of B. Bassiana spores was examined by observing development of 100 blastopores randomly and determined under light microscope with 400 times magnification. Observation was conducted in two replicates after the spores of B. bassiana were kept in the storage for 2, 4, 8 and 16 weeks. The result showed that by adding 1 g tapioca flour and temperature of storage of 5oC was potentiall in keeping viability of B. bassianaspores at least for 2 months. It was due to that tapioca flour gave better effect than rice and maize flours in keeping the storage and appropriate low temperature. Viability of B. bassianaspores decreased with increasing carrier dosage, temperature and duration of the storage. Whereas, storage at 5oC was found to be a better condition in keeping viability of dry pure B. bassianaspores longer than conditions of 23o and 29oC. Key word:Beauveria bassiana, temperature, viability,carrier.

  12. Lethal effects of a Mexican Beauveria bassiana (Balsamo) strain against Meccus pallidipennis (Stal)

    Science.gov (United States)

    Lino, Zumaquero Rios José; Juventino, López-Tlacomulco José; Raúl, Rojas García; Estibaliz, Sansinenea

    2014-01-01

    The entomopathogenic fungus Beauveria bassiana (Balsamo 1835) Vuillemin is an effective alternative control agent against some agricultural pests and biological vectors of important diseases such as Chagas disease. In this work we studied an isolate of Beauveria bassiana from of the town of San Antonio Rayón, Puebla, Mexico and its entomopathogenic effects on Meccus pallidipennis (Stal 1872). Phylogenetic analysis using molecular comparison of the ITS and EF1α genes, showed that the resulting cladogram places the BUAP 04 strain with a relationship closer to the AFAO 9-6 strain, within the diversity of the B. bassiana sensu lato group. Although there was the possibility that BUAP 04 strain was a direct descendant of strains used in campaigns of biologic control, molecular study allowed us to recognize that it was a different fungus due to numerous inserts. A strain isolated from a T. dimiata was evaluated for pathogenicity against another triatoma (Meccus pallidipennis) species obtaining an LC50 of 4.16 × 106 spores/mL, confirming that the BUAP 04 strain is virulent for M. pallidipennis and could be a good prospect for formulations to control M. pallidipennis. PMID:25242941

  13. Lethal effects of a Mexican Beauveria bassiana (Balsamo strain against Meccus pallidipennis (Stal

    Directory of Open Access Journals (Sweden)

    Zumaquero Rios José Lino

    2014-06-01

    Full Text Available The entomopathogenic fungus Beauveria bassiana (Balsamo 1835 Vuillemin is an effective alternative control agent against some agricultural pests and biological vectors of important diseases such as Chagas disease. In this work we studied an isolate of Beauveria bassiana from of the town of San Antonio Rayón, Puebla, Mexico and its entomopathogenic effects on Meccus pallidipennis (Stal 1872. Phylogenetic analysis using molecular comparison of the ITS and EF1α genes, showed that the resulting cladogram places the BUAP 04 strain with a relationship closer to the AFAO 9-6 strain, within the diversity of the B. bassiana sensu lato group. Although there was the possibility that BUAP 04 strain was a direct descendant of strains used in campaigns of biologic control, molecular study allowed us to recognize that it was a different fungus due to numerous inserts. A strain isolated from a T. dimiata was evaluated for pathogenicity against another triatoma (Meccus pallidipennis species obtaining an LC50 of 4.16 x 10(6 spores/mL, confirming that the BUAP 04 strain is virulent for M. pallidipennis and could be a good prospect for formulations to control M. pallidipennis.

  14. Lethal effects of a Mexican Beauveria bassiana (Balsamo) strain against Meccus pallidipennis (Stal).

    Science.gov (United States)

    Lino, Zumaquero Rios José; Juventino, López-Tlacomulco José; Raúl, Rojas García; Estibaliz, Sansinenea

    2014-01-01

    The entomopathogenic fungus Beauveria bassiana (Balsamo 1835) Vuillemin is an effective alternative control agent against some agricultural pests and biological vectors of important diseases such as Chagas disease. In this work we studied an isolate of Beauveria bassiana from of the town of San Antonio Rayón, Puebla, Mexico and its entomopathogenic effects on Meccus pallidipennis (Stal 1872). Phylogenetic analysis using molecular comparison of the ITS and EF1α genes, showed that the resulting cladogram places the BUAP 04 strain with a relationship closer to the AFAO 9-6 strain, within the diversity of the B. bassiana sensu lato group. Although there was the possibility that BUAP 04 strain was a direct descendant of strains used in campaigns of biologic control, molecular study allowed us to recognize that it was a different fungus due to numerous inserts. A strain isolated from a T. dimiata was evaluated for pathogenicity against another triatoma (Meccus pallidipennis) species obtaining an LC50 of 4.16 × 10(6) spores/mL, confirming that the BUAP 04 strain is virulent for M. pallidipennis and could be a good prospect for formulations to control M. pallidipennis.

  15. PATOGENICIDADE DE BEAUVERIA BASSIANA (BALS.) VUILL. AO ÁCARO Tetranychus urticae KOCH

    OpenAIRE

    Tamai Marco Antonio; Alves Sérgio Batista; Neves Pedro Janeiro

    1999-01-01

    O estudo da patogenicidade de Beauveria bassiana (Bals.) Vuill. ao ácaro Tetranychus urticae Koch foi desenvolvido a 25 ± 2°C, 70 ± 5% UR e 12 horas de fotofase, utilizando-se fêmeas recém-emergidas. Os ácaros foram mantidos durante seis dias em discos de folha de Canavalia ensiformis (L.) DC. inoculadas com B. bassiana, isolado 447, nas concentrações: 5x106, 1x107, 5x107, 1x108, 5x108 e 1x109 conídios/ml. Este isolado mostrou-se patogênico a T. urticae, apresentando aumento nos valores das m...

  16. THE EFFECT OF SELECTED PESTICIDES ON THE GROWTH OF ENTOMOPATHOGENIC FUNGI HIRSUTELLA NODULOSA AND BEAUVERIA BASSIANA

    Directory of Open Access Journals (Sweden)

    Cezary Tkaczuk

    2015-06-01

    Full Text Available The effect of three pesticides used in corn protection: Targa Super 05 (chizalofop-P-ethyl, Roundup 360SL (glyphosate and Karate Zeon 050CS (lambda-cyhalothrin, on the growth of entomopathogenic fungi Hirsutella nodulosa Petch and Beauveria bassiana (Bals. Vuill. was evaluated under laboratory conditions. Fungi isolates were cultured on Sabouraud’s medium with addition of pesticides at three different concentrations. H. nodulosa was more susceptible to pesticides than B. bassiana. The most inhibitory effect on tested entomopathogenic fungi showed chizalofop-P-ethyl herbicide. Lambda-cyhalothrin at the recommended field dose showed minor toxic effect on entomopathogenic fungi, which suggests the possibility of compatible use of this insecticide with biopesticides based on the tested species.

  17. Efficacy of Molasses and Beauveria bassiana (Balsamo Vuill on the Control of Cosmopolites sorditus Germar, 1824

    Directory of Open Access Journals (Sweden)

    Flávia Queiroz de Oliveira

    2010-06-01

    Full Text Available This research sought to study the compatibility between the molasses and the entomopathogenic fungi Beauveria bassiana (Balsamo Vuill on the control of Cosmopolites sorditus Germar, 1824. The study was conducted on the Site Pitiá in a banana plantation of approximately 250 m2, located in the municipality of Areia, state of Paraiba, Brazil. The results showed that the constancy of C. sorditus was significantly higher in traps consisting of molasses+fungi (P <0.01. And significantly lower in traps without yeast and molasses. The frequency of C. sorditus was classified as accidental in traps with only B. bassiana (23.44% and in traps without the addition of B. bassiana and molasses (10.71%, and being accessory in traps consisting of molasses (28.43% and in traps with molasses+fungi (37.27%. Therefore the food bait molasses along with the fungi B. bassiana, may be used in an integrated control of C. sorditus in banana plantations.

  18. Effect of Leaf Litters and Soils on Viability of Entomopathogenic Fungi Beauveria bassiana (Bals. Vuill

    Directory of Open Access Journals (Sweden)

    LISDAR IDWAN SUDIRMAN

    2008-09-01

    Full Text Available Viability of Beauveria bassiana is extremely low due to toxic compounds in soils. This research was aimed to study the effect of four groups of media on viability of B. bassiana Bb-Pb2. The first group was leaf litters of onion, flowering white cabbage, cabbage, and chinese mustard, respectively; the second group was the soils containing decomposed residues of each plant of the first group; the third group was the mixtures of each media of both groups above (1:1, and the fourth group was natural top soil as a control. Each plastic bag filled with one kg of each medium was inoculated with ten ml of B. bassiana conidia (106/ml of concentration and incubated in open area for 8 weeks. The results showed that all leaf litters of those plants and their compost soils affected the fungal viability. The highest decreasing number of colony was found on onion’s leaf litters, soil containing of decomposed onion, and the mixtures of both media. The treated B. bassiana showed significant reducing abilities of growth, conidia production and conidia germination on PDA media, except the one of control. It is suggested that the Bb-Pb2 isolate might not be effective as bioinsecticide in the soils containing either those leaf litters or composts.

  19. Cloning and expression in Saccharomyces cerevisiae of chit2 gene from Beauveria bassiana

    Institute of Scientific and Technical Information of China (English)

    SONG Jin-zhu; YANG Xiao-xue; WANG Yun; YANG Qian

    2009-01-01

    To study recycled trashes from shrimps and crabs in the sea through chitinase secreted by microor-ganisms, the chitinase gene chit2 was cloned and sequenced from Beauveria bassiana by the polymerase chain reaction (PCR), and was ligated into the yeast expression vector pYES2. The expression vector plasmid was transformed into Saccharomyces cerevisiae H158. Gene expression took place upon induction with 2% galac-tose. The measurement of enzyme activity shows that the expression production can be expressed in active forms and secreted to the medium. The enzyme activity approaches the peak of 0. 63 U/mL when the culture time is 36 h.

  20. Field evaluation of the efficacy of neem oil (Azadirachta indica A. Juss) and Beauveria bassiana (Bals.) Vuill. in cotton production

    NARCIS (Netherlands)

    Togbe, C.E.; Haagsma, R.; Zannou, E.; Gbehounou, G.; Déguénon, J.M.; Vodouhe, S.; Kossou, D.; Huis, van A.

    2015-01-01

    Neem oil (Azadirachta indica A. Juss) alone and combined with the entomopathogenic fungus Beauveria bassiana (Balsamo) Vuillemin (isolate Bb11) was applied to control cotton pests. The efficacy of these treatments was compared with that of synthetic insecticides applied either in a calendar-based ap

  1. Synergistic interaction between the fungus Beauveria bassiana and desiccant dusts applied against poultry red mites (Dermanyssus gallinae)

    DEFF Research Database (Denmark)

    Steenberg, Tove; Kilpinen, Ole Østerlund

    2014-01-01

    , but these fungi often take several days to kill mites. Laboratory experiments were carried out to study the efficacy of 3 types of desiccant dusts, the fungus Beauveria bassiana and combinations of the two control agents against D. gallinae. There was significant synergistic interaction between each...

  2. Evaluation of Beauveria bassiana (Hyphomycetes) isolates as potential agents for control of Dendroctonus valens

    Institute of Scientific and Technical Information of China (English)

    Long-Wa Zhang; Yu-Jun Liu; Jian Yao; Bin Wang; Bo Huang; Zeng-Zhi Li; Mei-Zhen Fan; Jiang-Hua Sun

    2011-01-01

    The red turpentine beetle (RTB), Dendroctonus valens LeConte, as a destructive invasive pest, has become one of the most economically important forest pest in China. Effective control measures are desperately needed. Entomopathogenic fungi, such as Beauveria bassiana, have shown great potential for the management of some bark beetle species. In this study, 12 isolates of B. bassiana from bark beetle were examined for biological characteristics and virulence, to assess their potential as biocontrol agents for RTB. There were significant differences (at P = 0.05) in colony growth rate, conidial yield, conidial germination, tolerance to UV light and extracellular proteases activity among the tested B. bassiana isolates. Isolates, including Bbl801, Bbl906, Bb789 and Bb773, exhibited the best characteristics, because they have faster hyphal growth rate, higher spore production and faster spore germination, higher UV tolerance and protease (Prl) production. The results of a pathogenicity test of B. bassiana on RTB larvae showed that most isolates of B. bassiana have demonstrated high efficacy and the highest virulent isolate was Bbl801, which killed 100% of the treated insects and had a median lethal time (LT50) of 4.60 days at a concentration of 1× 107 conidia/mL. Therefore, isolate Bb 1801 has a great potential for sustainable control of RTB in the forest. The correlation between biological characteristics and virulence of the fungal isolates is discussed and the possibility of combination of entomopathogenic fungi with semiochemicals, as one of the promising strategy for RTB control, is considered.

  3. Susceptibility of Tetranychus urticae Koch. (Acari: Tetranychidae to Isolates of Entomopathogenic Fungus Beauveria bassiana

    Directory of Open Access Journals (Sweden)

    Slavimira A. Draganova

    2010-01-01

    Full Text Available Bioassays with five isolates of the entomopathogenic fungus Beauveria bassiana (Bals.- Criv. Vuillemin were conducted under laboratory conditions with a goal to estimate their virulence to the two-spotted spider mite Tetranychus urticae Koch. (Acari: Tetranychidae. Common bean plants with mites were treated by spraying conidial suspensions of isolates at concentrations of 106, 107 and 108 conidia/ml. Lethal effects of the fungal isolates wereevaluated as percentages of cumulative daily mortality due to mycoses, corrected for mortality in the control variant. Virulence of the isolates was estimated based on values of the median lethal time (LT50 calculated by probit analysis for the variants treated with conidial suspensions at the concentration of 106 conidia/ml.The two-spotted spider mite was found susceptible to the examined isolates of the entomopathogenic fungus B. bassiana. Mycosis caused to T. urticae by the B. bassiana isolates 444 Bb and 445 Bb had fast lethal effect after treatment with conidial suspensions even at the concentration of 106 conidia/ml. The mean mortality values of host individuals were 83.78 ± 3.62% and 68.49 ± 4.28% on the first day, respectively, and up to 100% in both variants on the fourth day.The isolates 426 Bb, 444 Bb and 445 Bb of B. bassiana were highly virulent to two-spotted spider mites with values of the median lethal time varied within overlapped narrow confidence intervals from 0.122 to 1.084 days (average value 0.162 days, from 0.117 to 1.398 days (average value 0.146 days and from 0.106 to 1.162 days (average value 0.131 days,respectively. Significant differences regarding virulence of the three isolates at p-level < 0.05 could not been proved. The other two examined isolates were distinctly less virulent to T. urticae than these three B. bassiana isolates.

  4. Sensitivity of Beauveria bassiana conidia to abiotic factors after successive in vitro subcultures

    Directory of Open Access Journals (Sweden)

    Janaina Zorzetti

    2014-09-01

    Full Text Available The aim of this study was to evaluate the effect of in vitro successive subcultures of Beauveria bassiana isolates in different nutritional conditions on conidia heat and UV radiation tolerance and on sensitivity to azoxystrobin fungicide. The fungus was initially inoculated in Alphitobius diaperinus (Coleoptera: Tenebrionidae adults and after its development, the conidia were cultivated in PDA (potato dextrose agar and MPE (medium to produce Beauveria spp. conidia originating the first subculture 1st (A conidia, which were successively multiplied in their media until the 20th cultivation. Conidia taken from the 20th subculture were again inoculated in the insect and after sporulation they were once more multiplied in the media, producing the 1st (B conidia. For the tests, 1st (A, 10th, 20th and 1st (B conidia from the CG 152 and Unioeste 40 isolates were used. Successive subcultures and the medium nutritional conditions interfered on the fungus physiology, and the isolates showed different behaviors. The subcultures on PDA showed higher thermotolerance to Unioeste 40 in all subcultures and to CG 152 just after inoculation in the host. B. bassiana was susceptible to UV radiation, and this characteristic was influenced by the subcultures in different media. Both isolates were more tolerant to UV radiation when subcultivated on MPE, while just for the 40 Unioeste this tolerance increased after host inoculation. The B. bassiana sensitivity to azoxystrobin fungicide was affected after successive subcultures in both medium. Conidia originated in MPE were more resistant to fungicide and the host inoculation increased this tolerance. These results show that it is possible to enhance the fungi efficiency to control pests, through the selection of a virulent isolate, and handling their nutritional conditions during cultivation and host inoculation.

  5. Beauveria bassiana Strains for Biological Control of Cosmopolites sordidus (Germ. (Coleoptera: Curculionidae in Plantain

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    Marilene Fancelli

    2013-01-01

    Full Text Available The objective of this study was to select strains of Beauveria bassiana for controlling Cosmopolites sordidus (Germ. in plantain farms (cv. Terra of the “Recôncavo” and southern regions in the state of Bahia, Brazil. The virulence of 32 B. bassiana isolates against C. sordidus was determined under laboratory conditions. Three isolates (CNPMF 407, CNPMF 218, and CNPMF 416 were selected for evaluation under field conditions in plantations located in the counties of Mutuípe and Wenceslau Guimarães. Population of C. sordidus was estimated every 15 days by using pseudostem traps. The efficiency of the three strains of B. bassiana was compared to chemical control (carbofuran, 4g/trap and absence of control. Carbofuran caused around 90% of adult mortality after 12 months, with a reduction in the population of C. sordidus since the first evaluation. A low number of trapped insects was observed in the fungus-treated plots, suggesting the efficiency of the isolates in controlling the C. sordidus population. The strain CNPMF 218 was the most efficient in controlling C. sordidus adults in both locations, causing around 20% mortality, leading to 40% population size reduction after 12 months.

  6. Effect of surfactants and temperature on germination and vegetative growth of Beauveria bassiana

    Directory of Open Access Journals (Sweden)

    Lizzy A. Mwamburi

    2015-03-01

    Full Text Available Three non-ionic surfactants: Tween20, Tween80 and Breakthru® were screened for their effects on spore germination and mycelial growth rates and for their influence on three isolates of Beauveria bassianaspore germination at various temperatures. Tween20 and Tween80 were compatible with all the B. bassiana isolates in the germination studies, but inhibited germination at higher surfactant concentrations, irrespective of the conidial concentrations. Breakthru® had an inhibitory effect on germination even at the lowest concentration of 0.1% on all the B. bassiana isolates. The effects of the surfactants on spore germination did not correspond with their effects on colony growth. Conidial viability within the same formulation declined significantly with increases in temperature, irrespective of the surfactant. The optimal temperature for conidial germination of B. bassiana isolates was approximately 25 °C with an upper limit at 30 °C. Isolate 7320 was identified as the least affected by the different surfactants. This isolate was able to germinate rapidly in a broad temperature range of 25–30 °C after 24 h, this characteristic being an essential factor in controlling house fly populations in poultry houses.

  7. Seleção de isolados de Metarhizium anisopliae e Beauveria bassiana patogênicos a soldados de Atta bisphaerica e Atta sexdens rubropilosa em condições de laboratório Selection of Metarhizium anisopliae and Beauveria bassiana isolates pathogenic to Atta bisphaerica and Atta sexdens rubropilosa soldiers under laboratory conditions

    OpenAIRE

    Alzimiro Marcelo Conteiro Castilho; Marcelo Elias Fraga; Elen de Lima Aguiar-Menezes; Carlos Alberto da Rocha Rosa

    2010-01-01

    As formigas do gênero Atta são pragas importantes de diversas culturas agrícolas, pastagens e reflorestamentos. Os fungos entomopatogênicos estão entre os fatores naturais de mortalidade dessas formigas e por isso apresentam potencial para serem usados no controle biológico dessa praga. O presente trabalho teve como objetivo selecionar isolados de Metarhizium anisopliae e Beauveria bassiana patogênicos a soldados de Atta bisphaerica e Atta sexdens rubropilosa em condições de laboratório. Para...

  8. The mosquito melanization response is implicated in defense against the entomopathogenic fungus Beauveria bassiana.

    Directory of Open Access Journals (Sweden)

    Hassan Yassine

    Full Text Available Mosquito immunity studies have focused mainly on characterizing immune effector mechanisms elicited against parasites, bacteria and more recently, viruses. However, those elicited against entomopathogenic fungi remain poorly understood, despite the ubiquitous nature of these microorganisms and their unique invasion route that bypasses the midgut epithelium, an important immune tissue and physical barrier. Here, we used the malaria vector Anopheles gambiae as a model to investigate the role of melanization, a potent immune effector mechanism of arthropods, in mosquito defense against the entomopathogenic fungus Beauveria bassiana, using in vivo functional genetic analysis and confocal microscopy. The temporal monitoring of fungal growth in mosquitoes injected with B. bassiana conidia showed that melanin eventually formed on all stages, including conidia, germ tubes and hyphae, except the single cell hyphal bodies. Nevertheless, melanin rarely aborted the growth of any of these stages and the mycelium continued growing despite being melanized. Silencing TEP1 and CLIPA8, key positive regulators of Plasmodium and bacterial melanization in A. gambiae, abolished completely melanin formation on hyphae but not on germinating conidia or germ tubes. The detection of a layer of hemocytes surrounding germinating conidia but not hyphae suggested that melanization of early fungal stages is cell-mediated while that of late stages is a humoral response dependent on TEP1 and CLIPA8. Microscopic analysis revealed specific association of TEP1 with surfaces of hyphae and the requirement of both, TEP1 and CLIPA8, for recruiting phenoloxidase to these surfaces. Finally, fungal proliferation was more rapid in TEP1 and CLIPA8 knockdown mosquitoes which exhibited increased sensitivity to natural B. bassiana infections than controls. In sum, the mosquito melanization response retards significantly B. bassiana growth and dissemination, a finding that may be exploited to

  9. Protection of metal artefacts with the formation of metal-oxalates complexes by Beauveria bassiana.

    Directory of Open Access Journals (Sweden)

    Edith eJoseph

    2012-01-01

    Full Text Available Several fungi present high tolerance to toxic metals and some are able to transform metals into metal-oxalate complexes. In this study, the ability of Beauveria bassiana to produce copper oxalates was evaluated in vitro. Growth performance was tested on various copper-containing media. B. bassiana proved highly resistant to copper, tolerating concentrations of up to 20 g.L-1, and precipitating copper oxalates on all media tested. Chromatographic analyses showed that this species produced oxalic acid as sole metal chelator. The production of metal-oxalates can be used in the restoration and conservation of archaeological and modern metal artefacts. The production of copper-oxalates was confirmed directly using metallic pieces (both archaeological and modern. The conversion of corrosion products into copper oxalates was demonstrated as well. In order to assess whether the capability of B. bassiana to produce metal-oxalates could be applied to other metals, iron and silver were tested as well. Iron appears to be directly sequestered in the wall of the fungal hyphae forming oxalates and probably goethite. However, the formation of a homogeneous layer on the object is not yet optimal. Silver nitrate was extracellularly reduced into nanoparticles of elemental silver by an unknown mechanism. The production of copper oxalates is immediately applicable for the conservation of copper-based artefacts. For iron and silver this is not yet the case. However, the vast ability of B. bassiana to transform toxic metals using different immobilization mechanisms seems to offer considerable possibilities for industrial applications, such as the bioremediation of contaminated soils or the green synthesis of chemicals.

  10. Protection of Metal Artifacts with the Formation of Metal-Oxalates Complexes by Beauveria bassiana.

    Science.gov (United States)

    Joseph, Edith; Cario, Sylvie; Simon, Anaële; Wörle, Marie; Mazzeo, Rocco; Junier, Pilar; Job, Daniel

    2011-01-01

    Several fungi present high tolerance to toxic metals and some are able to transform metals into metal-oxalate complexes. In this study, the ability of Beauveria bassiana to produce copper oxalates was evaluated. Growth performance was tested on various copper-containing media. B. bassiana proved highly resistant to copper, tolerating concentrations of up to 20 g L(-1), and precipitating copper oxalates on all media tested. Chromatographic analyses showed that this species produced oxalic acid as sole metal chelator. The production of metal-oxalates can be used in the restoration and conservation of archeological and modern metal artifacts. The production of copper oxalates was confirmed directly using metallic pieces (both archeological and modern). The conversion of corrosion products into copper oxalates was demonstrated as well. In order to assess whether the capability of B. bassiana to produce metal-oxalates could be applied to other metals, iron and silver were tested as well. Iron appears to be directly sequestered in the wall of the fungal hyphae forming oxalates. However, the formation of a homogeneous layer on the object is not yet optimal. On silver, a co-precipitation of copper and silver oxalates occurred. As this greenish patina would not be acceptable on silver objects, silver reduction was explored as a tarnishing remediation. First experiments showed the transformation of silver nitrate into nanoparticles of elemental silver by an unknown extracellular mechanism. The production of copper oxalates is immediately applicable for the conservation of copper-based artifacts. For iron and silver this is not yet the case. However, the vast ability of B. bassiana to transform toxic metals using different immobilization mechanisms seems to offer considerable possibilities for industrial applications, such as the bioremediation of contaminated soils or the green synthesis of chemicals. PMID:22291684

  11. Relationship between Biological Characteristics of Beauveria bassiana (Bals.) Vuill and Pathogenicity to Bombyx mori L.%Relationship between Biological Characteristics of Beauveria bassiana (Bals.) Vuill and Pathogenicity to Bombyx mori L.

    Institute of Scientific and Technical Information of China (English)

    Haiyu LUO; Yecheng DENG; Yongmei LIAO; Ruiyu LI

    2012-01-01

    [Objective] This study was to investigate the relationship between biological characteristics of Beauveria bassiana (Bals.) Vuill and pathogenicity to Bombyx rnori L, with the aim to provide scientific basis for the control of white muscardine in Bombyx mori L. [Method] The strains isolated and purified from the 6 Beauveria bassiana biocontrol agents from all over the country and the 3 white muscardine silkworms collected from Guangxi provincial silkworm rearing areas were identified by the morphological observation and molecular biology technology. The pathogenicity of B. bassaina to silkworms was determined, and the biological characteristics such as growth diameter, sporulation and the extracellular protease activity of the different B. bassiana strains were compared. [Result] The isolated 9 strains were all B. bassaina (Bals.) Vuillemin, and all strains had high pathogenicity to silkworm, but with different pathogenicities. The growth diameter, sporulation and extracellular protease activity of different B. bassiana strains were also different, and showed correlation with the patheogenicity to silkworms. [Conclusion] B. bassiana spores production amount and exocellular protease activity had significant positive correlation with their pathogenicity to silkworm.

  12. Field evaluation of the synergistic effects of neem oil with Beauveria bassiana (Hypocreales: Clavicipitaceae) and Bacillus thuringiensis var. kurstaki (Bacillales: Bacillaceae)

    NARCIS (Netherlands)

    Togbe, C.E.; Zannou, E.; Gbehounou, G.; Kossou, D.; Huis, van A.

    2014-01-01

    In the present study, the synergistic effects of Beauveria bassiana (Bals.-Criv. Vuill.) (isolate Bb11) and Bacillus thuringiensis var. kurstaki (Berliner) with neem oil were evaluated in three agroecological zones in Be´nin. Four bioinsecticide treatments (neem oil, neem oil and B. bassiana used se

  13. Evaluation of the efficacy of beauveria bassiana for the control of the invasive fruit fly bactrocera invadens (Diptera: Tephritidae)

    International Nuclear Information System (INIS)

    Mango production plays an important role in Africa’s economy. However, the African invader fly, Bactrocera invadens is causing high yield losses as an important quarantine pest. Suppression of fruit flies for increased mango production will increasingly rely on management methods which exert low negative environmental impact. Beauveria bassiana is an insect pathogenic fungus used as microbial insecticide because it leaves produce to their fresh state, flavor, colour and texture with no change in the chemical composition of the product and is environmentally friendly. Evaluation of the efficacy of Beauveria bassiana for the control of the invasive Fruit Fly, Bactrocera invadens (Diptera: Tephriitidae) was carried out. The fungus B. bassiana (Botanigard® ES) containing 11.3% Beauveria bassiana GHA strain was applied at concentrations of 106, 53.0, 26.5, 13.3 and 6.65(x 106 spores/ml). When three developmental stages of the fruit fly (larvae, puparia and adults) were treated with Beauveria bassiana, the severity of the damage caused by the fungus increased with increasing fungal concentration. The results show lethal time (LT50) that ranged from 2.8 to 3.6 days for a dose of 106 x 106 spores/ml. Comparing methods of fungal application in the field, the result indicated that applying the fungus in fruit fly traps in mango canopies is the better method for fruit flies control in the field as compared to the soil surface spray method. However, both methods could be employed for better results The study of gamma radiation on the virulence of the fungus showed that the combined effect of the fungus and gamma irradiation gave better result by increasing adult mortality to 100 % within three days at 106 x106 spores/ml irradiated at 150 Gy than applying fungal treatment only. (author)

  14. Effect of moisture content and inoculum on the growth and conidia production by Beauveria bassiana on wheat bran

    OpenAIRE

    Oscar Nuñez-Gaona; Gerardo Saucedo-Castañeda; Raquel Alatorre-Rosas; Octavio Loera

    2010-01-01

    The aim of the present work was to study the effect of moisture content and inoculum on the growth and conidia production by Beauveria bassiana on wheat bran (WB).The highest growth rate of B. bassiana 885.2 on WB media was obtained at a w=1.0, with no detected growth at a w < 0.97. Solid-state fermentation (SSF) using WB (66% moisture; a w=1.0) achieved a maximal yield of 1.18x10 10 conidia per gram of dry substrate (gds). This yield decreased one order of magnitude with higher moisture cont...

  15. Optimization of Solid-State Fermentation for Improved Conidia Production of Beauveria bassiana as a Mycoinsecticide.

    Science.gov (United States)

    Pham, Tuan Anh; Kim, Jeong Jun; Kim, Keun

    2010-06-01

    The production of conidia of entomopathogenic Beauveria bassiana by solid-state fermentation was studied for the development of a biocontrol agent against aphid Myzus persicae. The optimal conditions for conidia production on polished white rice were 40% moisture content, 25℃ culture temperature, 2-day-old seeding culture grown in 3% corn meal, 2% rice bran, 2% corn steep powder medium, initial conidia concentration of 10(7) conidia/g in the wet rice, 10% inoculum size, and use of a polyethylene bag as a container. The polyethylene bag containing inoculated rice was hand-shaken every 12 hr during fermentation. Using optimal conditions, the maximum conidia production obtained was 4.05 g conidia/100 g dry rice after 14 days of cultivation, a rate 2.83 times higher than conidia yield of pre-optimization. PMID:23956642

  16. Virulencia de Beauveria bassiana y Metarhizium anisopliae sobre picudo del nopal Metarnasius spinolae

    OpenAIRE

    Orduño Cruz, Nuvia

    2012-01-01

    El picudo del nopal (Metamasius spinolae), es uno de los principales factores limitantes de la producción comercial de nopal en Tlalnepantla Morelos. El control de plagas se basa en insecticidas y el uso de nuevas alternativas como los hongos entomopatógenos ha sido poco estudiada. Con el objeto de seleccionar aislamientos de las especies B. bassiana y M. anisopliae para el control del picudo del nopal se evaluaron seis aislamientos de B. bassiana y dos de M. anisopliae en...

  17. Patogenicidade de Beauveria bassiana ao psilídeo Diaphorina citri e compatibilidade do fungo com produtos fitossanitários Beauveria bassiana pathogenicity to Diaphorina citri and compatibility of the fungus with phytosanitary products

    Directory of Open Access Journals (Sweden)

    Ana Paula Ferreira Pinto

    2012-12-01

    Full Text Available O objetivo deste trabalho foi avaliar a patogenicidade de Beauveria bassiana a ninfas de Diaphorina citri (Hemiptera: Psyllidae e verificar a compatibilidade do fungo com produtos fitossanitários e sua persistência em plantas de citros. Ninfas de D. citri foram pulverizadas com B. bassiana, nas concentrações 5x10(6, 1x10(7, 5x10(7, 1x10(8, 5x10(8 e 1x10(9 conídios mL-1, para determinação da concentração letal. Para avaliação da compatibilidade do fungo com produtos fitossanitários, extrato de nim e cinco inseticidas de quatro grupos químicos diferentes foram incorporados individualmente ao meio de cultura BDA em que o fungo foi cultivado. Avaliaram-se o crescimento vegetativo, a esporulação e a viabilidade do entomopatógeno. Plantas de citros, mantidas em casa de vegetação, foram tratadas primeiramente com os produtos fitossanitários e depois com o entomopatógeno. Avaliaram-se os tempos de exposição de 24 horas e de 7 e 14 dias. O fungo foi patogênico às ninfas de D. citri; a CL50 foi de 0,4x10(7 e a CL90 de 6,7x10(7 conidios mL-1, no décimo dia de avaliação. Em laboratório, os produtos fitosssanitários reduzem o crescimento do fungo. Em casa de vegetação, os produtos não afetam a sobrevivência do fungo nas plantas de citros.The objective of this work was to evaluate the pathogenicity of Beauveria bassiana to Diaphorina citri (Hemiptera: Psyllidae nymphs, and to check the compatibility of the fungus with phytosanitary products, and its persistence in citrus plants. Nymphs of D. citri were sprayed with B. bassiana in the concentrations 5x10(6, 1x10(7, 5x10(7, 1x10(8, 5x10(8 and 1x10(9 conidia mL-1 for lethal concentration determination. In order to evaluate the compatibility of the phytosanitary products with the fungus, neem extract and five insecticides of four different chemical groups were individually added to PDA culture medium, in which the fungus was cultivated. Vegetative growth, sporulation and viability

  18. 10株白僵菌菌株形态学与分子鉴定%Morphological and Molecular Identification of 10 Beauveria bassiana Strains

    Institute of Scientific and Technical Information of China (English)

    刘宝生; 谷希树; 田涛; 霍建飞; 孙淑琴

    2012-01-01

    对10株白僵菌属菌株的菌落形态、产孢结构和分生孢子形状、大小作了观察与测量,形态学鉴定为球孢白僵菌.同时对此10株球孢白僵菌核糖体DNA - ITS进行了测序分析,与NCBI数据库中的球孢白僵菌(Beauveria bassiana)多个菌株的同源性达99.10% ~99.64%.依此,10株白僵菌菌株分子鉴定结果为球孢白僵菌(Beauveria bassiana).%In this study, the colony morphology, spore structure and spore shape and size of 10 Beauveria strains were observed and measured, and they were initially identified as Beauveria bassiana. At the same time, their ribosomal DNA - ITS were sequenced and compared with that of Beauveria bassiana in NCBI database. The results showed the homology was 99.10% -99.64%. Eventually these 10 strains were identified as Beauveria bassiana.

  19. Use of Beauveria bassiana to control northern fowl mites (Ornithonyssus sylviarum) on roosters in an agricultural research facility.

    Science.gov (United States)

    Rassette, Matthew S W; Pierpont, Elizabeth I; Wahl, Tina; Berres, Mark

    2011-11-01

    Treatment of Northern fowl mite (Ornithonyssus sylviarum) infestation on poultry in research facilities can be challenging. The mite has a rapid reproductive cycle (egg to adult in 5 to 7 d), and chemical treatments can be toxic to birds, personnel, and the environment. In addition, antimite treatment may interfere with experimental research designs. The current study evaluated the efficacy of topical application of an entomopathogenic fungus, Beauveria bassiana, in the treatment of a naturally occurring infestation of Northern fowl mites in pen-housed roosters (n = 14; age, 18 mo). Two groups of 7 roosters each were used in 2 experiments: Beauveria (30 mL, 2.9 × 10(10) spores per bird) compared with water (30 mL, control), and Beauveria compared with the common topical organophosphate agent tetrachlorvinphos-dichlorvos (30 mL). We also assessed a higher dose of Beauveria (300 mL, 2.9 × 10(11) spores per bird) in the 7 birds that were not exposed to tetrachlorvinphos-dichlorvos. Beauveria reduced mite levels relative to the control group but did not outperform tetrachlorvinphos-dichlorvos when used at an equal volume and frequency. Increasing the volume and frequency of Beauveria application improved outcomes such that visual inspection failed to detect any mites. The results presented here suggest that, when applied in sufficient doses, Beauveria effectively reduces mites on poultry and can be an important part of an integrated pest management program. Additional research is needed to document the most effective dose, frequency, and location of B. bassiana application to control Northern fowl mites in poultry. PMID:22330786

  20. Sterile Males of Ceratitis Capitata (Diptera: Tephritidae) as Disseminators of Beauveria Bassiana Conidia for IPM Strategies

    International Nuclear Information System (INIS)

    Full text: Sterile Mediterranean fruit fly, Ceratitis capitata (Wied.), males were evaluated as vectors to spread Beauveria bassiana (Bals) conidia to wild C. capitata populations under field conditions. The inoculated sterile males were released by air, using the chilled adult technique over 7000 ha of coffee growing in Chimaltenango, Guatemala, Central America. The impact of releases was determined using dry traps baited with a food attractant. The effects of these releases on Apis mellifera, Linnaeus (honey bee), Hypothenemus hampei, Ferrari (coffee berry borer) and the parasitic mite Varroa destructor (Oudeman) were also evaluated. Inoculated sterile males were able to transmit fungal spores to 44% of the wild C. capitata flies captured in traps, which likely were infected through intra- and intersexual interactions during leks, mating or mating attempts. There was no transmission of the fungal spores to non- target insect species such as coffee berry borer, honey bees or varroa. We conclude that sterile males of Mediterranean fruit fly inoculated with B. bassiana can act as effective vectors of conidia to wild populations, constituting a safe, environmentally friendly and selective alternative for suppressing the medfly under a Sterile Insect Technique-based IPM approach. (author)

  1. Fungal Keratitis Due to Beauveria bassiana in a Contact Lenses Wearer and Review of Published Reports.

    Science.gov (United States)

    Lara Oya, Ana; Medialdea Hurtado, María Eloisa; Rojo Martín, María Dolores; Aguilera Pérez, Antonia; Alastruey-Izquierdo, Ana; Miranda Casas, Consuelo; Rubio Prats, Marina; Medialdea Marcos, Santiago; Navarro Marí, José María

    2016-10-01

    Fungal keratitis is a severe ocular infection that primarily affects subjects engaged in outdoor activities. Risk factors include allergic conjunctivitis, previous eye surgery, previous treatment with wide-spectrum antimicrobial agents and corticosteroids and using contact lenses. Corneal infection is usually secondary to trauma involving organic material, which is often the only predisposing factor. Early diagnosis based on clinical examination and microbiological investigation (microscopy, cultures and molecular techniques) is crucial to selecting the appropriate antifungal therapy and prevent progression. We report the case of a patient with keratitis due to Beauveria bassiana, an opportunistic and entomopathogenic filamentous fungus that is used as a biological insecticide and which is a rare cause of corneal infection. We review previous cases reports of B. bassiana keratitis published and its main features to compare with our case, a female occasional agriculture worker who had not suffered any trauma involving organic material. The patient received topical and oral antifungal therapy and debridement surgery, with a satisfactory outcome. PMID:27300341

  2. Proteins in the Cocoon of Silkworm Inhibit the Growth of Beauveria bassiana.

    Science.gov (United States)

    Guo, Xiaomeng; Dong, Zhaoming; Zhang, Yan; Li, Youshan; Liu, Huawei; Xia, Qingyou; Zhao, Ping

    2016-01-01

    Silk cocoons are composed of fiber proteins (fibroins) and adhesive glue proteins (sericins), which provide a physical barrier to protect the inside pupa. Moreover, other proteins were identified in the cocoon silk, many of which are immune related proteins. In this study, we extracted proteins from the silkworm cocoon by Tris-HCl buffer (pH7.5), and found that they had a strong inhibitory activity against fungal proteases and they had higher abundance in the outer cocoon layers than in the inner cocoon layers. Moreover, we found that extracted cocoon proteins can inhibit the germination of Beauveria bassiana spores. Consistent with the distribution of protease inhibitors, we found that proteins from the outer cocoon layers showed better inhibitory effects against B. bassiana spores than proteins from the inner layers. Liquid chromatography-tandem mass spectrometry was used to reveal the extracted components in the scaffold silk, the outermost cocoon layer. A total of 129 proteins were identified, 30 of which were annotated as protease inhibitors. Protease inhibitors accounted for 89.1% in abundance among extracted proteins. These protease inhibitors have many intramolecular disulfide bonds to maintain their stable structure, and remained active after being boiled. This study added a new understanding to the antimicrobial function of the cocoon. PMID:27032085

  3. Effect of temperature and humidity on pathogenicity of native Beauveria bassiana isolate against Musca domestica L.

    Science.gov (United States)

    Mishra, Sapna; Kumar, Peeyush; Malik, Anushree

    2015-12-01

    Beauveria bassiana HQ917687 virulence to housefly larvae and adult was assessed at different relative humidity, RH (50, 75, 90, and 100 %) and temperature (15, 20, 25, 30, 35, 40, 45 °C) conditions at the fungal dose of 10(8) conidia/ml. Depending on the temperature and RH regime tested, difference in mortality rates of housefly adult and larvae were detected. During assay on adult housefly, 100 % mortality was achieved at RH, 90 and 100 % while the temperature of 30 °C showed maximum mortality at all the tested humidity conditions. Lethal time, LT50 was 2.9 days at 100 % RH. Larval mortality at different humidity conditions varied between 30 and 74 %, with maximum mortality at 100 % RH and 30 °C. Optimum temperature for B. bassiana virulence to housefly larvae was also found to be 30 °C. The interaction between temperature and RH revealed significant effect of RH at moderate temperature range (20-35 °C), while such an interaction was not observed at extreme temperatures. The results obtained in this study have useful implications in understanding the pathogen behavior under actual field conditions. This in turn may help devising suitable entomopathogen release schedules for maximum fungal infection.

  4. Beauveria bassiana Lipase A expressed in Komagataella (Pichia pastoris with potential for biodiesel catalysis

    Directory of Open Access Journals (Sweden)

    Ana Claudia Vici

    2015-10-01

    Full Text Available Lipases (EC 3.1.1.3 comprise a biotechnologically important group of enzymes because they are able to catalyze both hydrolysis and synthesis reactions, depending on the amount of water in the system. One of the most interesting application of lipase is in the biofuel industry for biodiesel production by oil and ethanol (or methanol transesterification. Entomopathogenic fungi, which are potential source of lipases, are still poorly explored in biotechnological processes. The present work reports the heterologous expression and biochemical characterization of a novel Beauveria bassiana lipase with potential for biodiesel production. The His-tagged B. bassiana lipase A (BbLA was produced in Komagataella pastoris in Buffered Methanol Medium (BMM induced with 1% methanol at 30 °C. Purified BbLA was activated with 0.05% Triton X-100 and presented optimum activity at pH 6.0 and 50°C. N-glycosylation of the recombinant BbLA accounts for 31.5% of its molecular weight. Circular dichroism and molecular modeling confirmed a structure composed of α-helix and β-sheet, similar to α/β hydrolases. Immobilized BbLA was able to promote transesterification reactions in fish oil, demonstrating potential for biodiesel production. BbLA was successfully produced in Komagataella pastoris and shows potential use for biodiesel production by the ethanolysis reaction.

  5. PATOGENICIDADE DE BEAUVERIA BASSIANA (BALS. VUILL. AO ÁCARO Tetranychus urticae KOCH

    Directory of Open Access Journals (Sweden)

    Tamai Marco Antonio

    1999-01-01

    Full Text Available O estudo da patogenicidade de Beauveria bassiana (Bals. Vuill. ao ácaro Tetranychus urticae Koch foi desenvolvido a 25 ± 2°C, 70 ± 5% UR e 12 horas de fotofase, utilizando-se fêmeas recém-emergidas. Os ácaros foram mantidos durante seis dias em discos de folha de Canavalia ensiformis (L. DC. inoculadas com B. bassiana, isolado 447, nas concentrações: 5x106, 1x107, 5x107, 1x108, 5x108 e 1x109 conídios/ml. Este isolado mostrou-se patogênico a T. urticae, apresentando aumento nos valores das mortalidades acumuladas (total, corrigida e confirmada à medida que a suspensão de conídios se tornou mais concentrada. Nas seis concentrações testadas, os valores de mortalidade corrigida ao sexto dia, foram inferiores a 50%, sendo observada apenas na concentração de 1x109 conídios/ml, mortalidade total superior a 50%.

  6. Proteins in the Cocoon of Silkworm Inhibit the Growth of Beauveria bassiana

    Science.gov (United States)

    Zhang, Yan; Li, Youshan; Liu, Huawei; Xia, Qingyou; Zhao, Ping

    2016-01-01

    Silk cocoons are composed of fiber proteins (fibroins) and adhesive glue proteins (sericins), which provide a physical barrier to protect the inside pupa. Moreover, other proteins were identified in the cocoon silk, many of which are immune related proteins. In this study, we extracted proteins from the silkworm cocoon by Tris-HCl buffer (pH7.5), and found that they had a strong inhibitory activity against fungal proteases and they had higher abundance in the outer cocoon layers than in the inner cocoon layers. Moreover, we found that extracted cocoon proteins can inhibit the germination of Beauveria bassiana spores. Consistent with the distribution of protease inhibitors, we found that proteins from the outer cocoon layers showed better inhibitory effects against B. bassiana spores than proteins from the inner layers. Liquid chromatography-tandem mass spectrometry was used to reveal the extracted components in the scaffold silk, the outermost cocoon layer. A total of 129 proteins were identified, 30 of which were annotated as protease inhibitors. Protease inhibitors accounted for 89.1% in abundance among extracted proteins. These protease inhibitors have many intramolecular disulfide bonds to maintain their stable structure, and remained active after being boiled. This study added a new understanding to the antimicrobial function of the cocoon. PMID:27032085

  7. Beauveria bassiana Lipase A expressed in Komagataella (Pichia) pastoris with potential for biodiesel catalysis

    Science.gov (United States)

    Vici, Ana C.; da Cruz, Andrezza F.; Facchini, Fernanda D. A.; de Carvalho, Caio C.; Pereira, Marita G.; Fonseca-Maldonado, Raquel; Ward, Richard J.; Pessela, Benevides C.; Fernandez-Lorente, Gloria; Torres, Fernando A. G.; Jorge, João A.; Polizeli, Maria L. T. M.

    2015-01-01

    Lipases (EC 3.1.1.3) comprise a biotechnologically important group of enzymes because they are able to catalyze both hydrolysis and synthesis reactions, depending on the amount of water in the system. One of the most interesting applications of lipase is in the biofuel industry for biodiesel production by oil and ethanol (or methanol) transesterification. Entomopathogenic fungi, which are potential source of lipases, are still poorly explored in biotechnological processes. The present work reports the heterologous expression and biochemical characterization of a novel Beauveria bassiana lipase with potential for biodiesel production. The His-tagged B. bassiana lipase A (BbLA) was produced in Komagataella pastoris in buffered methanol medium (BMM) induced with 1% methanol at 30°C. Purified BbLA was activated with 0.05% Triton X-100 and presented optimum activity at pH 6.0 and 50°C. N-glycosylation of the recombinant BbLA accounts for 31.5% of its molecular weight. Circular dichroism and molecular modeling confirmed a structure composed of α-helix and β-sheet, similar to α/β hydrolases. Immobilized BbLA was able to promote transesterification reactions in fish oil, demonstrating potential for biodiesel production. BbLA was successfully produced in K. pastoris and shows potential use for biodiesel production by the ethanolysis reaction. PMID:26500628

  8. Preparation, characterization, and insecticidal activity evaluation of three different formulations of Beauveria bassiana against Musca domestica.

    Science.gov (United States)

    Mishra, Sapna; Kumar, Peeyush; Malik, Anushree

    2013-10-01

    Three formulations; bait, encapsulation, and emulsion of Beauveria bassiana were prepared and evaluated for their insecticidal activity in simulated field settings. Tea waste-based bait formulation of B. bassiana showed 100% mortality (within 72 h) in lab assay against adult houseflies. In field assay using traps, 65% relative entrapment and 100 % mortality (within 60 h) of entrapped flies was observed. Although the bait formulation was low cost and easy to prepare and transport, its storage ability was limited. Hence, more advanced formulations in form of encapsulation and emulsion was attempted. Encapsulated B. bassiana conidia (using skimmed milk powder, polyvinyl pyrrolidone K-90 and glucose as additives) showed 100% conidial germination and retained 78% conidial viability, even after storage for 12 months at 30 °C. Encapsulated product showed 54.8% (freshly prepared) and 30.6 % (after 12-months storage) mortality of housefly larvae in a simulated field condition. Emulsion formulation was prepared by using Tween 20 as surfactant with seven vegetable oils: soybean, rapeseed, sunflower, olive, castor, til, and linseed. Emulsion with linseed oil showing maximum conidial germination (94%) was evaluated for shelf life and pathogenecity against housefly larvae. Shelf life analysis of emulsion revealed 28% conidial germination and 19.9% housefly larval mortality after 12 months of storage as opposed to 94% conidial germination and 51.7% of larval mortality with fresh product. Significant increase in shelf × targeted application of formulation is expected to increase its mass applicability for housefly control. Also, the variability among products presents diverse opportunities for commercialization.

  9. Screening of liquid media and fermentation of an endophytic Beauveria bassiana strain in a bioreactor.

    Science.gov (United States)

    Lohse, Rieke; Jakobs-Schönwandt, Desiree; Patel, Anant V

    2014-01-01

    A novel approach for biological control of insect pests could be the use of the endophytic entomopathogenic Beauveria bassiana isolate ATP-02. For the utilization of the endophyte as a commercial biocontrol agent, the fungus has to be mass-produced. B. bassiana was raised in shake flask cultures to produce high concentrations of total spores (TS), which include blastospores (BS) and submerged conidiospores (SCS). The highest concentration of 1.33×10(9) TS/mL and the highest yield of 5.32×10(10) TS/g sucrose was obtained in the TKI broth with 5% sugar beet molasses which consists of 50% sucrose as a carbon source. In spite of the lower sugar concentration (2.5%) the amount of TS could be increased up to 11-times in contrast to the cultivation with 5% sucrose. The scale-up to a 2 L stirred tank reactor was carried out at 25°C, 200-600 rpm and 1 vvm at pH 5.5. A TS yield of 5.2×10(10) TS/g sucrose corresponding to a SCS yield of 0.2×10(10) SCS/g sucrose was obtained after 216 h. With regards to the culture medium the cost of 10(12) TS amounts to 0.24 €. Plutella xylostella larvae, which were fed with oilseed rape leaves treated with spores from fermentation resulted in 77 ± 5% mortality. Moreover, spores from submerged cultivation were able to colonize oilseed rape leaves via leaf application. This is the first report of fermentation of an endophytic B. bassiana strain in a low-cost culture medium to very high yields of TS. PMID:24949278

  10. Effect of biofertilizers and neem oil on the entomopathogenic fungi beauveria bassiana (Bals.) vuill. and metarhizium anisopliae (Metsch.) sorok.

    OpenAIRE

    Hirose Edson; Neves Pedro M. O. J.; Zequi João A. C.; Martins Luís H.; Peralta Cristiane H.; Moino Jr. Alcides

    2001-01-01

    The in vitro fungitoxic effect of three biofertilizers, E.M.-4, Multibion Ô and Supermagro used in organic agriculture and the neem oil (Azadirachta indica A. Juss) on the entomopathogenic fungi Metarhizium anisopliae and Beauveria bassiana was studied. These products were mixed in a medium where the two fungi were inoculated, and germination, vegetative growth and conidiogenesis were assessed. The biofertilizers Supermagro and E.M.- 4 showed to be less toxic for the two fungi whereas Multibi...

  11. Use of Beauveria bassiana to Control Northern Fowl Mites (Ornithonyssus sylviarum) on Roosters in an Agricultural Research Facility

    OpenAIRE

    Rassette, Matthew SW; Pierpont, Elizabeth I.; Wahl, Tina; Berres, Mark

    2011-01-01

    Treatment of Northern fowl mite (Ornithonyssus sylviarum) infestation on poultry in research facilities can be challenging. The mite has a rapid reproductive cycle (egg to adult in 5 to 7 d), and chemical treatments can be toxic to birds, personnel, and the environment. In addition, antimite treatment may interfere with experimental research designs. The current study evaluated the efficacy of topical application of an entomopathogenic fungus, Beauveria bassiana, in the treatment of a natural...

  12. Diversity of Fungi Associated with Atta bisphaerica (Hymenoptera: Formicidae): The Activity of Aspergillus ochraceus and Beauveria bassiana

    OpenAIRE

    Ribeiro, Myriam M. R.; Karina D. Amaral; Vanessa E. Seide; Souza, Bressane M. R.; Della Lucia, Terezinha M. C.; Maria Catarina M. Kasuya; Danival J. de Souza

    2012-01-01

    The grass-cutting ant Atta bisphaerica is one of the most serious pests in several pastures and crops in Brazil. Fungal diseases are a constant threat to these large societies composed of millions of closely related individuals. We investigated the occurrence of filamentous fungi associated with the ant A. bisphaerica in a pasture area of Viçosa, Minas Gerais State, Brazil. Several fungi species were isolated from forager ants, and two of them, known as entomopathogenic, Beauveria bassiana an...

  13. External development of the entomopathogenic fungi Beauveria bassiana and Metarhizium anisopliae in the subterranean termite Heterotermes tenuis Desenvolvimento dos fungos entomopatogênicos Beauveria bassiana E Metarhizium anisopliae no cupim subterrâneo Heterotermes tenuis

    Directory of Open Access Journals (Sweden)

    Alcides Moino Jr.

    2002-06-01

    Full Text Available The subterranean termite Heterotermes tenuis is one of the main pests of sugarcane and eucalyptus in Brazil, and the use of entomopathogenic fungi, alone or associated to chemicals, is an efficient and environmentally favorable method for its control. Studies related to the fungal development on these insects are important due to the effect of insect behavior on entomopathogens. The objective of this work was to describe the external development of Beauveria bassiana and Metarhizium anisopliae on H. tenuis using Scanning Electron Microscopy (SEM, determining the duration of the different phases of fungal infection. Two fixation techniques for preparing SEM samples were also evaluated. Worker specimens of H. tenuis were inoculated with a 1 x 10(9 conidia mL-1 suspension of the fungi and maintained at 25±1ºC and 70±10% relative humidity. Insects were collected from 0 to 144 hours after inoculation and prepared on SEM stubs for each of the two fixation techniques. The results obtained with the two techniques were compared and duration of the different phases of the infection process were estimated from SEM observations and compared for three fungal isolates. B. bassiana and M. anisopliae have similar development cycles on the termite, but some important differences exist. The penetration, colonization and conidiogenesis phases are relatively faster for M. anisopliae than for B. bassiana, which results in a faster rate of insect mortality. The fixation technique with OsO4 vapor is suitable for preparation of insects to be used in SEM observation of the developmental stages of entomopathogenic fungi.O cupim subterrâneo Heterotermes tenuis , uma das principais pragas da cana-de-açúcar e eucalipto no Brasil, e o uso de fungos entomopatogênicos, isoladamente ou associados a produtos químicos, é um método eficiente e ambientalmente seguro para seu controle. Estudos relacionados ao desenvolvimento fúngico nestes insetos são importantes devido

  14. Laboratory and semi-field evaluation of Beauveria bassiana (Ascomycota: Hypocreales) against the lettuce aphid, Nasonovia ribisnigri (Hemiptera: Aphididae)

    DEFF Research Database (Denmark)

    Shrestha, Govinda; Enkegaard, Annie; Steenberg, Tove

    2015-01-01

    The lettuce aphid, Nasonovia ribisnigri (Mosley), is an economically important pest of lettuce worldwide. The entomopathogenic fungus Beauveria bassiana strain GHA has recently been reported as a potential biocontrol candidate for use against the lettuce aphid. This study provides information...... on the mortality inflicted by B. bassiana when applied against different life stages of the lettuce aphid under laboratory conditions and how fungus infection affects the aphid fecundity. In addition, temporal changes in persistence of fungus inoculum applied to foliage of young lettuce plants under semi......-field conditions was analysed. Immature life stages were generally the least susceptible to fungal infection and the susceptibility of all stages was dose-dependent, with the highest mortality occurring at the highest dose. B. bassiana significantly affected the rate of nymph production by the lettuce aphid...

  15. Improved shelf life of dried Beauveria bassiana blastospores using convective drying and active packaging processes.

    Science.gov (United States)

    Mascarin, Gabriel Moura; Jackson, Mark A; Behle, Robert W; Kobori, Nilce N; Júnior, Ítalo Delalibera

    2016-10-01

    The yeast form (blastospore) of the dimorphic insect-pathogenic fungus Beauveria bassiana can be rapidly produced using liquid fermentation methods but is generally unable to survive rapid dehydration processes or storage under non-refrigerated conditions. In this study, we evaluated the influence of two convective drying methods, various modified atmosphere packaging systems, and storage temperatures on the desiccation tolerance, storage stability, and virulence of blastospores of B. bassiana ESALQ 1432. All blastospore formulations were dried to air drying and spray drying was greater than 80 %. Vacuum-packaged blastospores remained viable longer when stored at 4 °C compared with 28 °C with virtually no loss in viability over 9 months regardless the drying method. When both oxygen and moisture scavengers were added to sealed packages of dried blastospore formulations stored at 28 °C, viability was significantly prolonged for both air- and spray-dried blastospores. The addition of ascorbic acid during spray drying did not improve desiccation tolerance but enhanced cell stability (∼twofold higher half-life) when stored at 28 °C. After storage for 4 months at 28 °C, air-dried blastospores produced a lower LC80 and resulted in higher mortality to whitefly nymphs (Bemisia tabaci) when compared with spray-dried blastospores. These studies identified key storage conditions (low aw and oxygen availability) that improved blastospore storage stability at 28 °C and will facilitate the commercial development of blastospores-based bioinsecticides. PMID:27198727

  16. Nutritional optimization of a native Beauveria bassiana isolate (HQ917687) pathogenic to housefly, Musca domestica L.

    Science.gov (United States)

    Mishra, Sapna; Malik, Anushree

    2013-10-01

    A new Beauveria bassiana isolate, showing high activity against Musca domestica L. (Diptera: Muscidae) adults (mortality-100.0 %), larvae (mortality-72.3 %) and pupae (Infection in emerged flies-96.7 %) was used. The isolate was subjected to a combinational approach towards selection of process parameters for its growth optimization. Initial screening of several carbon and nitrogen sources revealed glucose and NaNO3 as the most suitable source for optimal biomass and spore production. Further, optimization through Placket-Burman and a 2(5) full factorial central composite design revealed highly significant effect of glucose and pH. The optimum composition for maximum biomass yield was (g/l): glucose 28; NaNO3 2.43; KH2PO4 1.32; MgSO4 0.60; and pH 7.00. Glucose concentration showed almost linear relationship with biomass yield, indicating its significant contribution in medium composition for fungal growth. Highly significant interactions were observed between glucose and pH, followed by glucose and NaNO3 concentration.

  17. Improving Efficacy of Beauveria bassiana against Stored Grain Beetles with a Synergistic Co-Formulant.

    Science.gov (United States)

    Storm, Clare; Scoates, Freya; Nunn, Adam; Potin, Olivier; Dillon, Aoife

    2016-01-01

    The potential of a dry powder co-formulant, kaolin, to improve the control of storage beetles by the entomopathogenic fungus Beauveria bassiana, isolate IMI389521, was investigated. The response of Oryzaephilus surinamensis adults to the fungus when applied to wheat at 1 × 10(10) conidia per kg with and without kaolin at 1.74 g per kg wheat was assessed. Addition of kaolin increased control from 46% to 88% at day 7 and from 81% to 99% at day 14 post-treatment. Following this the dose response of O. surinamensis and Tribolium confusum to both kaolin and the fungus was investigated. Synergistic effects were evident against O. surinamensis at ≥0.96 g of kaolin per kg of wheat when combined with the fungus at all concentrations tested. For T. confusum, adult mortality did not exceed 55%, however, the larvae were extremely susceptible with almost complete suppression of adult emergence at the lowest fungal rate tested even without the addition of kaolin. Finally, the dose response of Sitophilus granarius to the fungus at 15 and 25 °C, with and without kaolin at 1 g per kg of wheat, was examined. Improvements in efficacy were achieved by including kaolin at every fungal rate tested and by increasing the temperature. Kaolin by itself was not effective, only when combined with the fungus was an effect observed, indicating that kaolin was having a synergistic effect on the fungus. PMID:27571107

  18. Natural Occurrence of Beauveria bassiana (Balsamo Vuillemin (Hyphomycetes: Moniliales on Anticarsia gemmatalis Hübner (Lepidoptera: Noctuidae in the State of Mato Grosso do Sul

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    Camila Rossoni

    2013-07-01

    Resumo. Relata-se a ocorrência natural de um fungo entomopatogênico sobre a lagarta Anticarsia gemmatalis Hübner (Lepidoptera: Noctuidae em uma área de soja convencional situada no município de Dourados, Mato Grosso do Sul. A lagarta foi coletada a campo e levada ao laboratório de microbiologia da Universidade Federal da Grande Dourados (UFGD, onde permaneceu em câmera úmida por aproximadamente 7 dias. Posteriormente, o fungo foi isolado em meio de cultura (BDA para identificação da espécie do entomopatógeno. O fungo foi identificado como Beauveria bassiana (Balsamo Vuillemin (Hyphomycetes: Moniliales e, isso representa o primeiro registro de parasitismo, dessa espécie, sobre a lagarta-da-soja no Estado de Mato Grosso do Sul.

  19. Susceptibility of the Parasitoid Phymastichus coffea LaSalle (Hymenoptera: Eulophidae) to Beauveria bassiana under laboratory conditions; Susceptibilidad del parasitoide Phymastichus coffea LaSalle (Hymenoptera:Eulophidae) a Beauveria bassiana en condiciones de laboratorio

    Energy Technology Data Exchange (ETDEWEB)

    Castillo, Alfredo; Gomez, Jaime; Infante, Francisco [El Colegio de la Frontera Sur (ECOSUR), Chiapas (Mexico). Dept. de Entomologia Tropical], e-mail: acastill@ecosur.mx, e-mail: jgomez@ecosur.mx, e-mail: finfante@ecosur.mx; Vega, Fernando E. [United States Department of Agriculture (USDA), Beltsville, MD (United States). Agricultural Research Service. Sustainable Perennial Crops Lab.], e-mail: fernando.vega@ars.usda.gov

    2009-09-15

    The coffee berry borer, Hypothenemus hampei (Ferrari) (Coleoptera: Curculionidae), is the most important coffee pest worldwide. Beauveria bassiana is a generalist entomopathogenic fungus widely used by coffee farmers to control this pest and Phymastichus coffea LaSalle (Hymenoptera: Eulophidae) is an African endo parasitoid of H. hampei adults, recently imported to several Latin American and Caribbean countries to aid in the coffee berry borer control. The objective of this study was to determine if B. bassiana is detrimental to P. coffea. The susceptibility of the parasitoid was evaluated in terms of adult survivorship, mean lethal concentration (LC{sub 50}), mean lethal time (LT{sub 50}), reproduction and immature mortality. The main effect of the fungus resulted in reduction of adult longevity and mortality of 100% for immature stages of this parasitoid. The LC{sub 50} for adults was 0.11% equivalent to 9.53 x 10{sup 7} conidia/ml of B. bassiana and a LT{sub 50} of 29.4 h, equivalent to reduction of 22% of its normal longevity as an adult. P. coffea was capable of disseminating spores of B. bassiana to non-infected H. hampei adults, which could indirectly cause the death of its own progeny. These results could be valuable when considering the use of both organisms in the field, especially in an integrated pest management program. (author)

  20. Effect of Biofertilizers and Neem Oil on the Entomopathogenic Fungi Beauveria bassiana (Bals. Vuill. and Metarhizium anisopliae (Metsch. Sorok

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    Hirose Edson

    2001-01-01

    Full Text Available The in vitro fungitoxic effect of three biofertilizers, E.M.-4, Multibion Ô and Supermagro used in organic agriculture and the neem oil (Azadirachta indica A. Juss on the entomopathogenic fungi Metarhizium anisopliae and Beauveria bassiana was studied. These products were mixed in a medium where the two fungi were inoculated, and germination, vegetative growth and conidiogenesis were assessed. The biofertilizers Supermagro and E.M.- 4 showed to be less toxic for the two fungi whereas MultibionÔ caused major inhibition on M. anisopliae, with reductions in germination (-37.74%, colony diameter (-30.26% and conidiogenesis (-42.62%. Neem oil promoted a larger negative effect on B. bassiana, inhibiting germination (-45.27%, colony diameter (-36.62% and conidiogenesis (-84.93%.

  1. Potential of Metarrhizium anisopliae and Beauveria bassiana isolates and Neem oil to control the aphid Lipaphis erysimi (Kalt.) (Hemiptera: Aphididae)

    International Nuclear Information System (INIS)

    This work aimed to determine the efficiency of the entomopathogenic fungi Metarrhizium anisopliae and Beauveria bassiana to control the aphid Lipaphis erysimi (Kalt.) (Hemiptera:Aphididae) in kale Brassica oleracea var acephala D.C., as well as their compatibility with a neem oil formulation (NeemsetoR). Ten isolates of both fungi were tested and the most pathogenic ones were B. bassiana CG001 and M. anisopliae CG30 with 90% and 4.4 days, and 64% and 3.8 days of mortality and median lethal time, respectively. Bioassays with neem at concentrations of 0.5, 1.0 and 2.0% were done either by leaf discs dipping or spraying the aphids on the leaf discs. The neem spraying treatment at 2.0% provided 90% mortality. The use of B. bassiana isolate CG001 or M. anisopliae isolate CG30 with neem at 0.125, 0.25, and 0.5%, demonstrated that these isolates could have their spore viability or colony growth affected when exposed to neem concentrations higher than 0.25%. In absolute values, the isolates B. bassiana CG001 and M. anisopliae CG30 are the most virulent to L. erysimi, and could be utilized in the management of this pest. (author)

  2. Susceptibility of the Parasitoid Phymastichus coffea LaSalle (Hymenoptera: Eulophidae) to Beauveria bassiana under laboratory conditions

    International Nuclear Information System (INIS)

    The coffee berry borer, Hypothenemus hampei (Ferrari) (Coleoptera: Curculionidae), is the most important coffee pest worldwide. Beauveria bassiana is a generalist entomopathogenic fungus widely used by coffee farmers to control this pest and Phymastichus coffea LaSalle (Hymenoptera: Eulophidae) is an African endo parasitoid of H. hampei adults, recently imported to several Latin American and Caribbean countries to aid in the coffee berry borer control. The objective of this study was to determine if B. bassiana is detrimental to P. coffea. The susceptibility of the parasitoid was evaluated in terms of adult survivorship, mean lethal concentration (LC50), mean lethal time (LT50), reproduction and immature mortality. The main effect of the fungus resulted in reduction of adult longevity and mortality of 100% for immature stages of this parasitoid. The LC50 for adults was 0.11% equivalent to 9.53 x 107 conidia/ml of B. bassiana and a LT50 of 29.4 h, equivalent to reduction of 22% of its normal longevity as an adult. P. coffea was capable of disseminating spores of B. bassiana to non-infected H. hampei adults, which could indirectly cause the death of its own progeny. These results could be valuable when considering the use of both organisms in the field, especially in an integrated pest management program. (author)

  3. A technique for the prevention of greenhouse whitefly (Trialeurodes vaporariorum) using the entomopathogenic fungus Beauveria bassiana M130.

    Science.gov (United States)

    Kim, Chang-Su; Lee, Jung-Bok; Kim, Beam-Soo; Nam, Young-Ho; Shin, Kee-Sun; Kim, Jin-Won; Kim, Jang-Eok; Kwon, Gi-Seok

    2014-01-01

    The possibility of using hyphomycete fungi as suitable biocontrol agents against greenhouse whitefly has led to the isolation of various insect pathogenic fungi. Among them is Beauveria bassiana, one of the most studied entomopathogenic fungi. The objective of this study was to use B. bassiana M130 as an insecticidal agent against the greenhouse whitefly. M130 isolated from infected insects is known to be a biocontrol agent against greenhouse whitefly. Phylogenetic classification of M130 was determined according to its morphological features and 18S rRNA sequence analysis. M130 was identified as B. bassiana M130 and showed chitinase (342.28 units/ml) and protease (461.70 units/ml) activities, which were involved in the invasion of the host through the outer cuticle layer, thus killing them. The insecticidal activity was 55.2% in petri-dish test, 84.6% in pot test, and 45.3% in field test. The results of this study indicate that B. bassiana has potential as a biological agent for the control of greenhouse whitefly to replace chemical pesticides.

  4. Selection of Beauveria bassiana (Bals. Vuill. and Metarhizium anisopliae (Metsch. Sorok. for the control of the mite Mononychellus tanajoa (Bondar

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    Barreto Rodrigo Soares

    2004-01-01

    Full Text Available The green mite, Mononychellus tanajoa (Bondar (Acari: Tetranychidae, is considered to be one of the key pests in cassava, Manihot esculenta Crants, leading to considerable field losses. In this study, ten Beauveria bassiana (Bals. Vuill. and ten Metarhizium anisopliae (Metsch. Sorok. isolates were evaluated with regard to their potential as biological control agents against adult M. tanajoa females. The total mortality percentage of M. tanajoa caused by B. bassiana ranged from 13.0 to 97.0%, with confirmed mortality rates extending from 9.0 to 91.0% and LT50 varying from 4.2 to 17.0 days. The M. anisopliae isolates showed total mortality percentages ranging from 12.0 to 45.0% with confirmed mortality rates extending from 8.0 to 45.0%, and LT50 varying from 8.6 to 19.8 days. Lethal Concentrations (LC50 of 3.93 × 10(6 conidia mL-1 and 7.44 × 10(8 conidia mL-1 were determined for B. bassiana and M. anisopliae, respectively. B. bassiana isolate 645 was the most efficient, being an alternative for use in biological control programs against the cassava green mite.

  5. Transmission of Beauveria bassiana from male to female Aedes aegypti mosquitoes

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    Reyes-Villanueva Filiberto

    2011-02-01

    Full Text Available Abstract Background Resistance to chemical insecticides plus high morbidity rates have lead to rising interest in fungi as candidates for biocontrol agents of mosquito vectors. In most studies fungal infections have been induced by exposure of mosquitoes to various surfaces treated with conidia. In the present study eight Mexican strains of Beauveria bassiana were assessed against Aedes aegypti by direct exposure of females to 6 × 108 conidia ml -1 on a filter paper, afterwards, the transmission of the least and most virulent isolates was evaluated by mating behavior from virgin, fungus-contaminated male to females, to examine this ethological pattern as a new approach to deliver conidia against the dengue vector. Methods In an exposure chamber with a filter paper impregnated with 6 × 108 conidia ml -1 of the least and most virulent strains of B. bassiana, 6-8 day old males of A. aegypti were exposed for 48 hours, and then transferred individually (each one was a replicate to another chamber and confined with twenty healthy females of the same age. Clean males were used in controls. Survival, infection by true mating (insemination or by mating attempts (no insemination and fecundity were daily registered until the death of last female. Data analysis was conducted with proc glm for unbalanced experiments and means were separated with the Ryan test with SAS. Results All strains were highly virulent with LT50 ranging from 2.70 (± 0.29 to 5.33 (± 0.53 days. However the most (Bb-CBG2 and least virulent (Bb-CBG4 isolates were also transmitted by mating behavior; both killed 78-90% of females in 15 days after being confined with males that had previously been exposed for 48 hours to fungi. Of these mortality rates, 23 and 38% respectively, were infections acquired by copulations where insemination occurred. The LT50 for sexually-infected females were 7.92 (± 0.46 and 8.82 (± 0.45 days for both strains, while the one in control was 13.92 (± 0

  6. EFEITO ANTAGÔNICO DE Trichoderma sp. NO DESENVOLVIMENTO DE Beauveria bassiana (Bals. Vuill. e Metarhizium anisopliae (Metsch. Sorok ANTAGONISTIC EFFECT OF Trichoderma sp. ON THE DEVELOPMENT OF Beauveria bassiana (Bals. Vuill. AND Metarhizium anisopliae (Metsch. SOROK

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    Alcides Moino Jr.

    1999-01-01

    Full Text Available Este trabalho teve por objetivo avaliar o efeito de Trichoderma sp. no desenvolvimento de Beauveria bassiana e Metarhizium anisopliae. Trichoderma sp., B. bassiana (isolado 634 e M. anisopliae (isolado E-9 foram inoculados em meio BDA, com intervalos de 0, 48, 120 e 168 horas entre a inoculação de Trichoderma sp. e dos entomopatógenos. Avaliou-se o crescimento radial das colônias nos períodos de 48 e 120 horas após a inoculação de Trichoderma sp., sendo que este afetou o desenvolvimento dos entomopatógenos quando inoculado simultaneamente ou após 48 horas. B. bassiana e M. anisopliae desenvolveram-se normalmente quando inoculados 168 horas antes de Trichoderma sp.. Também foi avaliado o efeito de um extrato de Trichoderma sp. sobre os entomopatógenos, com a adição de 0,1; 0,5; 1,0 e 5,0 ml de extrato/100,0 ml de meio, onde foram inoculados os entomopatógenos. Foram medidos os diâmetros de colônias e o número de conídios produzidos por B. bassiana e M. anisopliae na presença do extrato. A concentração de 5,0 ml de extrato/100,0 ml de meio alterou o crescimento e a conidiogênese de B. bassiana. O fungo M. anisopliae foi afetado a partir da adição de 1,0 ml de extrato/100,0 ml de meio.The objective of this work was to evaluate the effect of Trichoderma sp. on the development of Beauveria bassiana and Metarhizium anisopliae. The fungus Trichoderma sp. was inoculated on PDA culture medium, 0, 48, 120 and 168 hours after inoculation of the same plates with either B. bassiana (isolate 634 or M. anisopliae (isolate E-9. The radial growth of fungal colonies was measured 48 and 120 hours after Trichoderma sp. inoculation. Trichoderma sp. affected the development of both entomopathogenic fungi when inoculated simultaneously or 48 hours later. B. bassiana and M. anisopliae had normal development when inoculated 168 hours before Trichoderma sp. The effect of a toxic extract from Trichoderma sp. on the entomopathogenic fungi was also

  7. Comparative efficacy of emulsifiable-oil, wettable-powder, and unformulated-powder preparations of Beauveria bassiana against the melon aphid Aphis gossypii

    Science.gov (United States)

    Aphicidal efficacy of two commercial formulations of Beauveria bassiana strain GHA conidia, an emulsifiable-paraffinic oil dispersion (OD) and a clay-based wettable powder (WP), were compared to unformulated conidia in laboratory tests with adult melon aphids (Aphis gossypii). For the initial 24 hou...

  8. A case of Beauveria bassiana keratitis confirmed by internal transcribed spacer and LSU rDNA D1–D2 sequencing

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    M. Ligozzi

    2014-05-01

    Full Text Available We describe a case of fungal keratitis due to Beauveria bassiana in a farmer with Fuchs' dystrophy, treated with amphotericin B. Surgery with penetrating keratoplasty was necessary to resolve the lesions. Susceptibility testing and molecular sequencing permitted the identification and treatment of this rare aetiological agent of invasive fungal disease.

  9. ENTOMOPATHOGENIC FUNGUS SPECIES BEAUVERIA BASSIANA (BALS. AND METARHIZIUM ANISOPLIAE (METSCH. USED AS MYCOINSECTICIDE EFFECTIVE IN BIOLOGICAL CONTROL OF IPS TYPOGRAPHUS (L.

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    Silvia Mudrončeková

    2013-06-01

    Full Text Available The agricultural world is overloaded with chemical substances. Undesirable effects and the resistance of vermin opens a new way for biological control of pathogenic species of animals. Ips typographus (L. is overgrowth across Central Europe. Its natural habitat are forest communities, where chemical spraying is forbidden or restricted. The natural bioregulators of this cosmopolitan pest are entomopathogenic fungi of Hypocreales species. In this study, we focused on two types of entomopathogenic fungi – Beauveria bassiana (BALS. and Metarhizium anisopliae (METSCH.. They are suitable for biological control because they are attacking a wide range of pathogenic insects in agro-systems. Entomopathogenic fungi were isolated from soil samples and dead infected insects. The samples collected from different sites of the High Tatras. In our experiments, we followed infectivity and mortality of selected isolates of entomopathogenic fungi Beauveria bassiana (BALS. and Metarhizium anisopliae (METSCH.. The gDNA of isolates was used for identification and we put them to the analysis of specific DNA segments by amplified PCR method. Results of experiments show the high pathogenicity of entomopathogenic fungi strains. Beauveria bassiana (BALS. caused 99% mortality and Metarhizium anisopliae (METSCH. reached 97%. Infectivity followed by Beauveria bassiana (BALS. reached 92% and Metarhizium anisopliae (METSCH. 90%. For the purpose of limiting the numbers of populations of harmful pest is essential the ability of entomopathogenic fungi to infect, kill the host, and remain in a natural environment without disturbing the biota.

  10. Two strains of Pseudomonas fluorscens bacteria differentially affect survivorship of waxworm (Galleria mellonella) larvae exposed to an arthropod fungal pathogen, Beauveria bassiana

    Science.gov (United States)

    Two strains of Pseudomonas fluorescens were found contaminating a biopesticide used in a previous study against Varroa destructor infestations in honey bee hives. In the aforementioned study the biopesticide, a formulation of the arthropod pathogen Beauveria bassiana, failed to have any impact on t...

  11. Impact of two treatments of a formulation of Beauveria bassiana (Deuteromycota: Hyphomycetes) conidia on Varroa mites (Acari: Varroidae) and on honeybee (Hymenoptera: Apidae) colony health

    Science.gov (United States)

    Bee colonies in southern France were treated with conidia from 2 strains of Beauveria bassiana – one commercial (GHA) and the other isolated from Varroa mites in the region (Bb05002). Objectives were to evaluate treatment effect on colony weight, adult bee mass, capped brood, and on Varroa fall onto...

  12. Ação do fungo Beauveria bassiana associado a gel polimerizado de celulose no controle do carrapato Anocentor nitens em teste de campo Action of the fungus Beauveria bassiana associated with cellulose polymerized gel in the control of the tick Anocentor nitens in a field test

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    E.J. Souza

    2009-02-01

    Full Text Available Foram selecionados 20 equídeos naturalmente infestados por Anocentor nitens, dos quais seus pavilhões auriculares foram submetidos a quatro tratamentos distintos: gel associado a Beauveria bassiana, apenas gel, apenas B. bassiana e um grupo-controle. Procederam-se à contagem e ao registro do número total de fêmeas adultas (>3mm em cada pavilhão auricular, nos dias 0, 1, 4, 7, 11, 14, 18, 21, 25 e 28, para cálculo do percentual de controle. Foram coletadas e mantidas em laboratório 20 fêmeas pertencentes a cada tratamento, para cálculo dos períodos de pré-postura, postura, incubação e eclosão, e peso médio das posturas. No grupo tratado com o composto gel associado a B. bassiana, observou-se percentual de controle maior que 50%, entre o 4º e o 25º dia, assim como decréscimo progressivo do peso médio da postura das fêmeas coletadas no período entre o 7º e 14º dia. Para os outros tratamentos, o percentual de controle manteve-se abaixo de 20%, e o peso da postura não apresentou diferença significativa. Os percentuais de controle, assim como as reduções no peso médio da postura, sugerem que a utilização do composto gel associado a B. bassiana potencializou a virulência do entomopatógeno.Twenty horses naturally infected by Anocentor nitens were selected and their auricular pinna were submitted to four treatments: gel associated with Beauveria bassiana, gel only, B. bassiana only, and a control group. The count and the register of the total number of grown up tick females (>3mm were performed in each auricular pinna on days 0, 1, 4, 7, 11, 14, 18, 21, 25, and 28 for the calculus of percentage of control. Twenty females from each treatment were collected and maintained in laboratory, to calculate the periods of pre-oviposition, oviposition, incubation, and hatching and mean weight of oviposition. In the group treated with the gel associated to B. bassiana, it was observed a control percentage higher than 50% from the 4º

  13. Evaluation of soyscreen in an oil-based formulation for UV protection of Beauveria bassiana conidia.

    Science.gov (United States)

    Behle, Robert W; Compton, David L; Laszlo, Joseph A; Shapiro-Ilan, David I

    2009-10-01

    Soyscreen oil was studied as a formulation ingredient to protect Beauveria bassiana (Balsamo) Vuillemin conidia from UV degradation. Feruloylated soy glycerides, referred to as Soyscreen oil, are biobased UV-absorbing molecules made by combining molecules of soybean oil with ferulic acid. Conidia stored in Soyscreen oil for 28 wk at 25, 30, and 35 degrees C retained viability as well as conidia stored in sunflower oil, demonstrating that Soyscreen did not adversely affect viability with prolonged storage. For samples applied to glass and exposed to simulated sunlight (xenon light), conidia in sunflower oil with or without sunscreens (Soyscreen or oxyl methoxycinnimate) had similar conidia viability after exposure. These oil formulations retained conidia viability better than conidia applied as an aqueous treatment. However, the 10% Soyscreen oil formulation applied to field grown cabbage (Brassica oleracea L.) and bean (Phaseolus vulgaris L.) plants, did not improve residual insecticidal activity compared with aqueous applications of unformulated conidia or two commercial formulations when assayed against Trichoplusia ni (Hübner) larvae. Our results suggest that the oil applications lose UV protection because the oil was absorbed by the leaf. This conclusion was supported in subsequent laboratory exposures of conidia in oil-based formulations with UV screens applied to cabbage leaves or balsa wood, which lost protection as measured by decreased viability of conidia when exposed to simulated sunlight. As a result, additional formulation techniques such as encapsulation to prevent separation of the protective oil from the conidia may be required to extend protection when oil formulations are applied in the field.

  14. [Effects of Beauveria bassiana on Myzus persicae and its two predaceous natural enemies].

    Science.gov (United States)

    Zhu, Hong; Luo, Xu-mei; Song, Jin-xin; Fan, Mei-zhen; Li, Zeng-zhi

    2011-09-01

    A Beauveria bassiana strain Bb21 was isolated from naturally infected green peach aphid Myzus persicae (Hemiptera: Aphididae). The effects of the strain on M. persicae and its two predaceous natural enemies Chrysoperla carnea (Neuroptera: Chrysopidae) and Harmonia axyridis (Coleoptera: Coccinellidae) were investigated under laboratory conditions. Bb21 had strong pathogenicity to M. persicae, with the LD50 of 97 conidia x mm(-2) (45-191, 95% confidence interval), but was less pathogenic to the second instar nymph of C. carnea, with the LD50 of 1089 conidia x mm(-2). The LD50 for C. carnea was 10.2 times higher than that for M. persicae. The pathogenicity of Bb21 to H. axyridis was very weak, with a low infection rate of 13% even at a high concentration 5 x 10(8) conidia x mL(-1). The Bb21 at low conidia concentration had less effect on the developmental period and fecundity of the two predaceous natural enemies. However, when applied at the high concentration 5 x 10(8) spores x mL(-1), Bb21 shortened the larval stage of H. axyridis averagely by 1.4 d and decreased the adult emergence rate and fecundity by 33% and 14%, respectively, and shortened the larval stage of C. carnea averagely by 0.7 d and decreased the adult emergence rate and fecundity by 24% and 11%, respectively. Since the LD50 for green peach aphid was much lower than that for the two predaceous natural enemies, and had very low effect on the adult emergence rate and fecundity of the two predators at the concentration recommended for field spray, Bb21 could be applied as a biocontrol agent of M. persicae in the integrated management of pernicious organisms.

  15. Evaluation of a New Entomopathogenic Strain of Beauveria bassiana and a New Field Delivery Method against Solenopsis invicta

    Science.gov (United States)

    Li, Jun; Guo, Qiang; Lin, Miaofeng; Jiang, Lu; Ye, Jingwen; Chen, Dasong; Li, Zhigang; Dai, Jianqing; Han, Shichou

    2016-01-01

    Solenopsis invicta Buren is one of the most important pests in China, and control measures are mainly based on the use of synthetic pesticides, which may be inadequate and unsustainable. Hence, there is a growing interest in developing biological control alternatives for managing S. invicta, such as the use of entomopathogenic fungi. To facilitate the commercialization of entomopathogenic fungi against S. invicta, 10 Beauveria bassiana isolates originating from different hosts were tested for virulence in laboratory bioassays, and the most pathogenic strain, ZGNKY-5, was tested in field studies using an improved pathogen delivery system. The cumulative mortality rate reached 93.40% at 1×108 mL-1 conidia after 504 h. The germination and invasion of the spores were observed under a scanning electron microscope, and several conidia adhered to the cuticle of S. invicta after 2 h. Furthermore, the germ tubes of the conidia oriented toward the cuticle after 48 h, and the mycelium colonized the entire body after 96 h. Based on the efficacy observed in the laboratory trials, further experiments were performed with ZGNKY-5 strain to evaluate its utility in an injection control technology against S. invicta in the field. We found that three dosage treatments of ZGNKY-5 strain (500 mL, 750 mL, and 1,000 mL per nest) had significant control effects. Our results show that this strain of Beauveria bassiana and our control method were effective against S. invicta in both laboratory and field settings. PMID:27341441

  16. Evaluation of a New Entomopathogenic Strain of Beauveria bassiana and a New Field Delivery Method against Solenopsis invicta.

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    Jun Li

    Full Text Available Solenopsis invicta Buren is one of the most important pests in China, and control measures are mainly based on the use of synthetic pesticides, which may be inadequate and unsustainable. Hence, there is a growing interest in developing biological control alternatives for managing S. invicta, such as the use of entomopathogenic fungi. To facilitate the commercialization of entomopathogenic fungi against S. invicta, 10 Beauveria bassiana isolates originating from different hosts were tested for virulence in laboratory bioassays, and the most pathogenic strain, ZGNKY-5, was tested in field studies using an improved pathogen delivery system. The cumulative mortality rate reached 93.40% at 1×108 mL-1 conidia after 504 h. The germination and invasion of the spores were observed under a scanning electron microscope, and several conidia adhered to the cuticle of S. invicta after 2 h. Furthermore, the germ tubes of the conidia oriented toward the cuticle after 48 h, and the mycelium colonized the entire body after 96 h. Based on the efficacy observed in the laboratory trials, further experiments were performed with ZGNKY-5 strain to evaluate its utility in an injection control technology against S. invicta in the field. We found that three dosage treatments of ZGNKY-5 strain (500 mL, 750 mL, and 1,000 mL per nest had significant control effects. Our results show that this strain of Beauveria bassiana and our control method were effective against S. invicta in both laboratory and field settings.

  17. Evaluation of kinetic parameters of chitinases produced by Beauveria bassiana (Bals. Vuill. / Avaliação de parâmetros cinéticos de quitinases produzidas por Beauveria bassiana (Bals. Vuill.

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    Cristiane Mita

    2008-07-01

    Full Text Available Entomopathogenic fungus Beauveria bassiana is currently used as a biocontrol agent for agricultural pests. The infection process involves extracellular enzymes such as proteases and chitinases that degrade the cuticle of the insects. The objective of this work was to evaluate kinetic parameters of pH, temperature, ionic concentration and time of reaction on chitinases activity. The fungus B. bassiana CG432 was cultivated on coffee berry borer Hypothenemus hampei (Ferrari and the conidia grown on insect were used to prepare the inoculum containing 108conídia/mL. These conidia were inoculated at 1% (v/v in culture liquid medium containing D-glucose (10g, yeast extract (5g, NaNO3 (1,58g, Na2HPO4.7H2O (1,05g, KCl (1g, MgSO4.7H2O (0,6g and KH2PO4 (0,36g per liter. The cultivation was carried at 28°C and 180rpm during 5 days. Culture fluid was obtained by filtration and centrifugation at 8.000g, and the chitinases were isolated and concentrated by ultrafiltration using 10 and 100kDa cut off membranes under nitrogen pressure. Chitinase activity was detected and quantified using N-acetylglucosamine released by hydrolysis of colloidal chitin at 40 to 60ºC, at 50, 100 and 200 mM ionic concentrations of buffers sodium acetate (pH 4.0 to 6.0; sodium phosphate (pH 6.0 to 8.0; and Glycine-NaOH (pH 8.0 to 10.0 during 60 minutes. Maximum chitinase activity was at 45ºC and pH 5.5, and was also high at pH 6.0 and pH 8.5 using 50mM buffer. The chitinase activity increased and was stable during an hour at optimum conditions of the reaction, shown the stable nature of this enzyme.Beauveria bassiana é um fungo entomopatogênico utilizado no controle biológico de insetos-praga que infestam produtos agrícolas. O mecanismo de infecção envolve a produção de enzimas extracelulares, como proteases e quitinases que degradam a cutícula dos insetos. O objetivo deste trabalho foi avaliar parâmetros cinéticos de pH, temperatura, concentração iônica e tempo de

  18. Pathogenicity of Metarrhizium anisopliae (Metsch.) Sorokin and Beauveria bassiana (Bals.) Vuillemin isolates to Scaptocoris carvalhoi Becker (Hemiptera, Cydnidae); Patogenicidade de isolados de Metarhizium anisopliae (Metsch.) Sorokin e de Beauveria bassiana (Bals.) Vuillemin a Scaptocoris carvalhoi Becker (Hemiptera, Cydnidae)

    Energy Technology Data Exchange (ETDEWEB)

    Xavier, Luciane Modenez Saldivar [Universidade Federal da Grande Dourados (UFGD), Dourados, MS (Brazil)], e-mail: luciane_modenez@ibest.com.br; Avila, Crebio Jose [EMBRAPA Agropecuaria Oeste, Dourados, MS (Brazil)], e-mail: crebio@cpao.embrapa.br

    2006-12-15

    Pathogenicity of the fungi Metarrhizium anisopliae (Metsch.) Sorokin and Beauveria bassiana (Bals.) Vuillemin to stink bug Scaptocoris carvalhoi Becker, 1967 was evaluated under laboratory and greenhouse conditions. Experiments were carried out at EMBRAPA Agropecuaria Oeste, Dourados, Mato Grosso do Sul State, Brazil, in 2003. Ten M. anisopliae and eleven B. bassiana isolates were evaluated in laboratory using a completely randomized experimental design with five replicates (10 adults and 5 nymphs/plot). The pathogenicity of M. anisopliae isolate (Ma69) was also separately evaluated against nymphs and adults in laboratory and greenhouse. The stink bug mortality levels were higher for M. anisopliae isolates (between 73.3% and 94.7% than for B. bassiana isolates (between 10.7% and 78.7%). In greenhouse, stink bug mortality due to the M. anisopliae isolate (Ma69) was 57.3%, and there was no difference of mortality for nymphs and adults of stink bug in laboratory. However, in greenhouse, mortality levels were significantly higher (p<0,05) for nymphs (38,4%) than for adults (16,2%). From these data, we conclude that M. anisopliae isolate Ma69 was efficient to control S. carvalhoi in laboratory and in greenhouse, thus being a promising choice for use as a microbial insecticide under field conditions. (author)

  19. Metabolic responses of Beauveria bassiana to hydrogen peroxide-induced oxidative stress using an LC-MS-based metabolomics approach.

    Science.gov (United States)

    Zhang, Chen; Wang, Wei; Lu, Ruili; Jin, Song; Chen, Yihui; Fan, Meizhen; Huang, Bo; Li, Zengzhi; Hu, Fenglin

    2016-06-01

    The entomopathogenic fungus, Beauveria bassiana, is commonly used as a biological agent for pest control. Environmental and biological factors expose the fungus to oxidative stress; as a result, B. bassiana has adopted a number of anti-oxidant mechanisms. In this study, we investigated metabolites of B. bassiana that are formed in response to oxidative stress from hydrogen peroxide (H2O2) by using a liquid chromatography mass spectrometry (LC-MS) approach. Partial least-squares discriminant analysis (PLS-DA) revealed differences between the control and the H2O2-treated groups. Hierarchical cluster analysis (HCA) showed 18 up-regulated metabolites and 25 down-regulated metabolites in the H2O2-treated fungus. Pathway analysis indicated that B. bassiana may be able to alleviate oxidative stress by enhancing lipid catabolism and glycometabolism, thus decreasing membrane polarity and preventing polar H2O2 or ROS from permeating into fungal cells and protecting cells against oxidative injury. Meanwhile, most of the unsaturated fatty acids that are derived from glycerophospholipids hydrolysis can convert into oxylipins through autoxidation, which can prevent the reactive oxygen of H2O2 from attacking important macromolecules of the fungus. Results showed also that H2O2 treatment can enhance mycotoxins production which implies that oxidative stress may be able to increase the virulence of the fungus. In comparison to the control group, citric acid and UDP-N-acetylglucosamine were down-regulated, which suggested that metabolic flux was occurring to the TCA cycle and enhancing carbohydrate metabolism. The findings from this study will contribute to the understanding of how the molecular mechanisms of fungus respond to environmental and biological stress factors as well as how the manipulation of such metabolisms may lead to selection of more effective fungal strains for pest control. PMID:27116916

  20. Ação in vitro dos fungos Beauveria bassiana (Bals Vuill e Metarhizium anisopliae (Metsch Sorok sobre ninfas e adultos de Amblyomma cajennense (Fabricius, 1787 (Acari: Ixodidae In vitro action of the fungi Beauveria bassiana (Bals Vuill and Metarhizium anisopliae (Metsch Sorok on ninphs and adults of Amblyomma cajennense (Fabricius, 1787 (Acari: Ixodidae

    Directory of Open Access Journals (Sweden)

    R.C.S. Reis

    2001-10-01

    Full Text Available O trabalho teve por objetivo verificar a mortalidade in vitro de ninfas e adultos de Amblyomma cajennense frente à ação de isolados dos fungos Beauveria bassiana e Metarhizium anisopliae. Foram avaliados três isolados de M. anisopliae (959, 319 e E9 e dois de B. bassiana (986 e 747. As suspensões de conídios foram preparadas a partir de fungos produzidos em meio de arroz, e cada bioensaio foi constituído de quatro tratamentos nas concentrações 10(5, 10(6, 10(7, 10(8 conídios/ml e um grupo-controle. A análise constou da observação do percentual de mortalidade, 15 dias após o tratamento ou após a ecdise de adultos. Foram observadas diferenças significativas entre os tratamentos quanto à mortalidade para todos os isolados e todos os instares. Nos grupos tratados houve aumento considerável na mortalidade à medida que se aumentava a concentração de conídios na suspensão. Conclui-se que todos os isolados testados causaram mortalidade em testes in vitro sobre esses estádios evolutivos, sugerindo o controle do A. cajennense pela ação desses fungos.This work aimed at the evaluation of the in vitro susceptibility of Amblyomma cajennense nimphs and adults to isolates of Beauveria bassiana and Metarhizium anisopliae fungi. Three isolates of M. anisopliae (959, 319 e E9 and two of B. bassiana (986 e 747 were used. Conidia suspensions were made from fungi grown up in a rice culture medium and each test consisted of four treated groups (10(5, 10(6, 10(7, 10(8 conidia/ ml plus a control group. Viability of individuals was assessed 15 days after treatment or after adult ecdisis. Significative differences were found for all treatments and for all stages studied. A large reduction in the viability of ticks was observed, and this effect increased as conidia concentration raised. Based upon the results obtained, it can be concluded that all isolates tested presented an in vitro lethality for the biological stages of A. cajennense evaluated

  1. A molecular tool for detection and tracking of a potential indigenous Beauveria bassiana strain for managing emerald ash borer populations in Canada.

    Science.gov (United States)

    Johny, Shajahan; Kyei-Poku, George

    2014-10-01

    Emerald ash borer is an invasive species from Asia. Beauveria bassiana strain L49-1AA is being tested for the control of emerald ash borer in Canada, using an autocontamination trapping system. We have developed a simplified allele discrimination polymerase chain reaction (PCR) assay to screen B. bassiana strain, L49-1AA from other Beauveria species by targeting the inter-strain genetic differences in 5' end of EF1-α gene of the genus Beauveria. A single nucleotide polymorphism (SNP) site, T→C was identified only in L49-1AA and was used to develop a simplified allele discrimination polymerase chain reaction (PCR) assay based on a modified allelic inhibition of displacement activity (AIDA) approach for distinguishing B. bassiana L49-1AA from all background Beauveria isolates. The SNP site was employed to design inner primers but with a deliberate mismatch introduced at the 3' antepenultimate from the mutation site in order to maximize specificity and detection efficiency. Amplification was specific to L49-1AA without cross-reaction with DNA from other Beauveria strains. In addition, the designed primers were also tested against environmental samples in L49-1AA released plots and observed to be highly efficient in detecting and discriminating the target strain, L49-1AA from both pure and crude DNA samples. This new method can potentially allow for more discriminatory tracking and monitoring of released L49-1AA in our autocontamination and dissemination projects for managing EAB populations. Additionally, the modified-AIDA format has potential as a tool for simultaneously identifying and differentiating closely related Beauveria species, strains/isolates as well as general classification of other pathogens or organisms. PMID:25110340

  2. Patogenicidade de Beauveria bassiana ao psilídeo Diaphorina citri e compatibilidade do fungo com produtos fitossanitários

    Directory of Open Access Journals (Sweden)

    Ana Paula Ferreira Pinto

    2012-12-01

    Full Text Available O objetivo deste trabalho foi avaliar a patogenicidade de Beauveria bassiana a ninfas de Diaphorina citri (Hemiptera: Psyllidae e verificar a compatibilidade do fungo com produtos fitossanitários e sua persistência em plantas de citros. Ninfas de D. citri foram pulverizadas com B. bassiana, nas concentrações 5x10(6, 1x10(7, 5x10(7, 1x10(8, 5x10(8 e 1x10(9 conídios mL-1, para determinação da concentração letal. Para avaliação da compatibilidade do fungo com produtos fitossanitários, extrato de nim e cinco inseticidas de quatro grupos químicos diferentes foram incorporados individualmente ao meio de cultura BDA em que o fungo foi cultivado. Avaliaram-se o crescimento vegetativo, a esporulação e a viabilidade do entomopatógeno. Plantas de citros, mantidas em casa de vegetação, foram tratadas primeiramente com os produtos fitossanitários e depois com o entomopatógeno. Avaliaram-se os tempos de exposição de 24 horas e de 7 e 14 dias. O fungo foi patogênico às ninfas de D. citri; a CL50 foi de 0,4x10(7 e a CL90 de 6,7x10(7 conidios mL-1, no décimo dia de avaliação. Em laboratório, os produtos fitosssanitários reduzem o crescimento do fungo. Em casa de vegetação, os produtos não afetam a sobrevivência do fungo nas plantas de citros.

  3. Diversity of Fungi Associated with Atta bisphaerica (Hymenoptera: Formicidae: The Activity of Aspergillus ochraceus and Beauveria bassiana

    Directory of Open Access Journals (Sweden)

    Myriam M. R. Ribeiro

    2012-01-01

    Full Text Available The grass-cutting ant Atta bisphaerica is one of the most serious pests in several pastures and crops in Brazil. Fungal diseases are a constant threat to these large societies composed of millions of closely related individuals. We investigated the occurrence of filamentous fungi associated with the ant A. bisphaerica in a pasture area of Viçosa, Minas Gerais State, Brazil. Several fungi species were isolated from forager ants, and two of them, known as entomopathogenic, Beauveria bassiana and Aspergillus ochraceus, were tested against worker ants in the laboratory. The two species were highly virulent, achieving 50 percent worker mortality within 4-5 days. It is the first time A. ochraceus, a commonly found fungal species, is reported to infect Atta species at a high prevalence. Possible uses for the fungus within biological control are discussed.

  4. Pathogenicity of three entomopathogenic fungi, Metarhizium anisopliae, Beauveria bassiana, and Paecilomyces lilacinus, to Tetranychus kanzawai infesting papaya seedlings

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    Yayan Sanjaya

    2016-09-01

    Full Text Available Tetranychus kanzawai is an economically important polyphagous mite species in East and South Asia. In the Philippines, it commonly infests cassava and papaya plants. The mites attack and severely damage the older leaves of papaya trees as well as seedlings. Its serious damage causes the leaves to dry up, thus, reducing the photosynthetic activity of the plant. Three entomopathogenic fungal isolates were tested on mites under greenhouse conditions using treated papaya seedlings following a completely randomized design. The mites tested were examined under a dissecting scope to determine the causal agent and to confirm mortality. The LT50 of Metarhizium anisopliae, Beauveria bassiana and Paecilomyces lilacinus on T. kanzawai were estimated. Our results indicate that among these entomopathogenic fungi, the Metarhizium anisopliae Ma6 isolate (LT50= 3.00 days has potentiality for the control of T. kanzawai.

  5. Study of increasing spore content of Beauveria bassiana%提高白僵菌孢子含量的研究

    Institute of Scientific and Technical Information of China (English)

    刘杰; 陈荣; 孙惠杰

    2001-01-01

    Adding some protein of Antheraea pernyi pupae as the replacement of powder of bean cake and meize flour into Beauveria bassiana raw material and culturing solid in three degrees in iron plate can significantly increase the spores content of Beauveria bassiana,reduce the cost and raise the effect.%在生产白僵菌的原料中,加入一定量的柞蚕蛹蛋白代替豆饼粉或玉米粉,采用铁盘三级固体培养,可显著提高白僵菌的含孢量、缩短生产周期、降低成本、提高对害虫的杀伤速率和效果。

  6. 温度对球孢白僵菌生物学特性的影响%Effects of Temperature on the Growth of Beauveria bassiana

    Institute of Scientific and Technical Information of China (English)

    祁楠

    2013-01-01

      研究了不同温度对球孢白僵菌生长的影响,结果表明在22~26益温度范围内球孢白僵菌落个数随温度的升高而逐渐增多,在26益时达到最大。随后随着温度的升高菌落个数逐渐降低。%This paper researched the influence of different temperatures,The results showed that the Beauveria bassiana colony di-ameter increased with temperature increasing at range of 22~26℃.At 26℃ the colony diameter were the biggest.then the Beauve-ria bassiana colony diameter decreased with temperature decreased.

  7. Experiment of Beauveria bassiana Against Dung Beetles in Sugarcane%白僵菌防治甘蔗金龟子试验

    Institute of Scientific and Technical Information of China (English)

    薛晶; 张会华; 杨晓丽; 何文志; 罗志明

    2012-01-01

    Experiment of Beauveria bassiana was conducted to control Dung beetles in sugarcane. The results indicated that con- trol effect could be 75% to 85% while applying Beauveria bassiana mixed up with 3.6% of Bisultap granular, which is obviously or significant greatly superior to this agent alone or other pesticides use.%试验结果表明,白僵菌与3.6%广谱型杀虫双颗粒剂混合施用,能有效控制金龟子幼虫对蔗根的危害,相对防效迭75%~85%,显著或极显著优于单独使用白僵菌或农药处理。

  8. Evaluating the virulence of Metarhizium anisopliae (Deuteromycotina: Hyphomycetes and Beauveria bassiana (Ascomycota: Hypocreales isolates to Arabian rhinoceros beetle, Oryctes agamemnon arabicus

    Directory of Open Access Journals (Sweden)

    M.W. Khudhair

    2015-12-01

    Full Text Available Virulence of entomopathogenic fungi Metarhizium anisopliae and Beauveria bassiana were tested against Arabian Rhinoceros Beetle, Oryctes agamemnon arabicus larvae. Four concentrations (1×105, 1×107, 1×109 and 1×1011 conidia/mL–1 of two locally isolated entomopathogenic fungi spore suspensions were used in this study via larval direct spraying. Results revealed that both isolates can cause high mortality rate reaching 100% after 29 days. However, Beauveria bassiana scored higher mortality rate in short time especially at the concentration of 1×1011 conidia/ mL–1 with lethal time (LT50 12.75 and LT90 20.00; while, Metarhizium anisopliae caused the higher percentage of malformed adults. Moreover, both isolates affected insect’s life cycle particularly in the pupal stage which was reduced remarkably by almost 50% in comparison with the control treatment.

  9. 应用白僵菌防治玉米螟的研究进展%Research Progress of Prevention and Controlling Corn Borer by Beauveria bassiana

    Institute of Scientific and Technical Information of China (English)

    陆晴; 佘花娣; 佟文悦

    2013-01-01

    The damage characteristics of corn borer, biological characteristics and pathogenic mechanism of Beauveria bassiana were introduced briefly.The present situation, problems and corresponding measures of prevention and controlling corn borer using Beauveria bassiana were summarized, and the new direction was discussed so as to promote the corn safety production and improve the control efficiency of corn borer.%简要介绍了玉米螟的为害特点以及白僵菌的生物学特性、致病机理,对利用白僵菌防治玉米螟的现状、存在问题及相应措施进行了阐述,并对防治玉米螟的新方向进行了展望,以促进玉米安全生产和提高玉米螟的防治效率。

  10. Effect of moisture content and inoculum on the growth and conidia production by Beauveria bassiana on wheat bran

    Directory of Open Access Journals (Sweden)

    Oscar Nuñez-Gaona

    2010-08-01

    Full Text Available The aim of the present work was to study the effect of moisture content and inoculum on the growth and conidia production by Beauveria bassiana on wheat bran (WB.The highest growth rate of B. bassiana 885.2 on WB media was obtained at a w=1.0, with no detected growth at a w < 0.97. Solid-state fermentation (SSF using WB (66% moisture; a w=1.0 achieved a maximal yield of 1.18x10 10 conidia per gram of dry substrate (gds. This yield decreased one order of magnitude with higher moisture contents or the addition of sugarcane bagasse (SCB as a texturiser. In SSF using WB (66% humidity,the time to obtain a yield of 1x10(10 conidia/gds, referred to as t10, could be predicted using a model considering common inoculum levels and maximal yields. For instance, t10 was 285 h with an inoculum of 1x10(6 conidia/gds; however, t10 was reduced to 232 h and 148 for inocula of 7x10(6 and 5x10(7 conidia/gds, respectively. The estimation of t10 values allowed both comparison between the cultures and prediction of harvesting times in production processes. Values for hydrophobicity were within 90 and 92%, whereas viability averages were around 70% for all the cultures

  11. Insecticidal effects of zinc oxide nanoparticles and Beauveria bassiana TS11 on Trialeurodes vaporariorum (Westwood, 1856 (Hemiptera: Aleyrodidae

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    Zahra KHOOSHE-BAST

    2016-10-01

    Full Text Available Greenhouse whitefly, Trialeurodes vaporariorum is a major pest of horticultural and ornamental plants and is usually controlled with insecticides or biological control agents. In the current study, we examined the effects of synthesized zinc oxide nanoparticles (ZnO NPs and Beauveria bassiana TS11 on T. vaporariorum adults. ZnO NPs were synthesized by precipitation method. Field emission scanning electron microscope images indicated that ZnO NPs were non-compacted uniformly. X-ray diffraction results confirmed the hexagonal wurtzite structure of ZnO NPs. Fourier transform infrared analysis showed an intense absorption peak at a range of 434-555 cm-1 related to Zn-O bond. In bioassays, adults were exposed to different concentrations of ZnO NPs (3, 5, 10, 15, 20 mg l-1 and fungi (104, 105, 106, 107, 108 spores ml-1. LC50 values for ZnO NPs and fungi were 7.35 mg l-1and 3.28×105 spores ml-1, respectively. Mortality rates obtained with ZnO NPs and fungi at the highest concentration were 91.6 % and 88.8 %, respectively. The results indicate a positive effect of ZnO NPs and B. bassiana TS11on adults. The current study was conducted under laboratory conditions, therefore, more studies are needed in field.

  12. Laboratory bioassay of Beauveria bassiana against Tetranychus urticae (Acari: Tetranychidae) on leaf discs and potted bean plants.

    Science.gov (United States)

    Ullah, Mohammad Shaef; Lim, Un Taek

    2015-03-01

    Use of the mycopathogen Beauveria bassiana (strain GHA), marketed as BotaniGard(®) ES, was evaluated as a plant protection strategy against the spider mite Tetranychus urticae Koch, which is considered one of the most economically important and cosmopolitan pests of many crops. Tetranychus urticae were treated with four concentrations of conidia (1 × 10(5), 1 × 10(6), 1 × 10(7), or 1 × 10(8) conidia/ml), and virulence was assessed on mites held at four relative humidity levels (35, 55, 75, and 95 ± 2 % RH) at 25 ± 1 °C. At 1 × 10(8) spores/ml, the LT50 value was 9.7 h at 95 % RH, which was significantly lower than values for other RH levels. At 1 × 10(7) spores/ml, the LT50 value was 43.8 h at 95 % RH, which was significantly different from values at 55 and 35 % RH. The efficacy of B. bassiana product was also verified on mites infesting potted bean plants with a concentration of 1 × 10(8) spores/ml. In double spray treatment where applications were made 2 × on days 5 and 10 after mite infestation, the nymphal and adult population of T. urticae were reduced to zero on days 20 and 15, respectively. With a single spray on day 5, the nymphal population was also greatly reduced, but increased rapidly after day 20. Single and double sprays with B. bassiana reduced leaf damage as measured by image analysis by 33 and 94 % compared to no treatment, respectively. These results suggest that 1 × 10(8) spores/ml was the most effective dose and that two applications, at a 5-day interval, provided control of T. urticae in our laboratory assay. PMID:25500970

  13. Pathogenicity of Beauveria bassiana isolated from Moroccan Argan forests soil against larvae of Ceratitis capitata (Diptera: Tephritidae) in laboratory conditions.

    Science.gov (United States)

    Imoulan, Abdessamad; Elmeziane, Abdellatif

    2014-03-01

    The Mediterranean fruit fly, Ceratitis capitata Wiedemann (Diptera: Tephritidae), is the major tephritid pest in Morocco. This pest survives in Moroccan forests Argania spinosa and continually invades the nearest agricultural areas. Entomopathogenic fungi are an interesting tool for fruit fly control and hold a useful alternative to conventional insecticides. However, primary selection of effective pathogens should be taken in laboratory condition prior to applying them in the field. Here, we used third late instar larvae of C. capitata to investigate the effectiveness of 15 local Beauveria bassiana isolates. Results showed that all isolates were able to infect the larval stage, producing a large mortality rate in puparia ranging from 65 to 95 % and caused significant reduction in adult emergence. The fungal treatments revealed that the mycosis occurred also in adults escaping infection as pupariating larvae. The percentage of mycosed puparia was highest in strain TAM6.2 (95 %) followed by ERS4.16 (90 %), therefore they were the most virulent. Median lethal concentration (LC₅₀) was studied for five isolates at four concentrations ranging from 10⁵ to 10⁸ conidia ml⁻¹. The results showed that the slopes of regression lines for B. bassiana ERS4.16 (slope = 0.386) and TAM6.2 (slope = 0.41) were the most important and had the lowest LC₅₀ values (2.85 × 10³ and 3.16 × 10³ conidia ml⁻¹ respectively). This investigation suggests that the soil of Argan forests contains pathogenic B. bassiana isolates and highlights for the first time their potential as biological control toward C. capitata larval stage in Morocco. PMID:24122125

  14. Evaluation of the pathogenicity of multiple isolates of Beauveria bassiana (Hypocreales: Clavicipitaceae) on Rhynchophorus ferrugineus (Coleoptera: Dryophthoridae) for the assessment of a solid formulation under simulated field conditions

    OpenAIRE

    Ricaño, Jorge; Güerri Agulló, Berenice; Serna Sarriás, María José; Rubio Llorca, Germán; Asensio Berbegal, Leticia; Barranco, Pablo; López Llorca, Luis Vicente

    2013-01-01

    A solid state formulation of Beauveria bassiana (Balsamo) Vuillemin has been developed for biological control of the Red Palm Weevil (RPW), Rhynchophorus ferrugineus (Olivier, 1790). Two kinds of bioassays (dry conidia and dipping) using 10 isolates from several coleopterans in Mediterranean environments, identified 2 RPW derived isolates (193 and 203) as most pathogenic to RPW larvae and adults (zero survival within first 4–5 d for dry conidia, and 14 and 23 d for dipping bioassays). Isolate...

  15. 森得保与白僵菌防治刚竹毒蛾对比试验%Effects of Sendebao and Beauveria Bassiana on Controlling Pantana phyllostachysae

    Institute of Scientific and Technical Information of China (English)

    詹祖仁; 陈锡桓; 张龙华; 张登强; 叶春珍

    2009-01-01

    采用森得保和白僵菌(Beauveria bassiana)两种生物农药在林间对刚竹毒蛾(Pantana phyllostachysae Chao)3~4龄幼虫进行防治对比试验,结果表明,防治效果分别为94.6%和59.4%,前者极显著高于后者.

  16. An attempt to use the fungus Beauveria bassiana (Bals.) Vuill. in forest protection against the bark beetle Ips typographus (L.) in the field

    OpenAIRE

    Grodzki Wojciech; Kosibowicz Mieczysław

    2015-01-01

    In 2011–2013, trials on the use of the entomopathogenic fungus Beauveria bassiana against bark beetle (Ips typographus) populations were carried out under open field conditions in Norway spruce stands suffering from an outbreak in the Beskid Żywiecki Mts. in Poland. Modified pheromone traps were deployed to capture and thereafter release fungus-infected bark beetles to the forest environment. Infested spruce trees felled next to the traps remained unaffected by the transmission of the fungus ...

  17. Beauveria bassiana (Bals. Vuill. spores production in biphasic process utilizing different liquid media/ Produção de esporos de Beauveria bassiana (Bals. Vuill. num processo bifásico utilizando diferentes meios líquidos

    Directory of Open Access Journals (Sweden)

    Janaína Zorzetti

    2005-06-01

    Full Text Available Entomopathogenic production techniques are developed with the objective of increasing productivity and reducing costs of the process. The objective of this study was to evaluate Beauveria bassiana biomass production in liquid media and conidiogenesis in a biphasic process. The liquid media were: CF (crysalid flour, PD (potato dextrose and CFPD (crysalid flour+potato+dextrose. The evaluation periods for biomass production were: 24, 48, 72, 96, 120 and 144 hours. For production by biphasic process different quantities of liquid media (5, 10, 15 and 20 mL with different incubation periods (24, 48 and 72 hours, were added on the pre-cooked rice (200 g with conidia production evaluation after 10 days. CFPD media was the most productive biomass in all evaluated periods. In the biphasic process, bigger conidia production were attained when it was utilized fungi biomass produced in CF and CFPD (2.7 x 1012 and 2.8 x 1012 conidia/g of rice for rice inoculation. The quantities of 5, 15, 20 mL of liquid media, inoculated on the rice were not statistically different. The incubation periods of 24 and 48 hours of the fungus in the liquid media, gave bigger cionidiogenesis then 72 hours. The data show the necessity of carbon and nitrogen for biomass production in the liquid media and for conidiogenesis in the solid media. Also with these informations it was possible to optimize the biphasic process, adding 5 mL of the liquid media CFPD on the rice (200 g with 24 hours of incubation.Técnicas de produção de fungos entomopatogênicos são desenvolvidas buscando aumentar a produtividade desses patógenos e reduzir custos do processo. O objetivo deste trabalho foi avaliar a produção de biomassa de Beauveria bassiana em meios líquidos e a conidiogênese no processo bifásico. Os meios líquidos testados foram: FC (farinha de crisálida, BD (batata+dextrose e FCBD (farinha de crisálida+batata+dextrose. Os períodos de avaliação da produção de biomassa foram

  18. A Distinctive Pattern of Beauveria bassiana-biotransformed Ginsenoside Products Triggers Mitochondria/FasL-mediated Apoptosis in Colon Cancer Cells.

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    Gum, Sang Il; Rahman, Md Khalilur; Won, Jong Soon; Cho, Min Kyung

    2016-01-01

    Ginseng is one of the most commonly used adaptogens. Transformation into the minor ginsenosides produces compounds with more effective action. Beauveria bassiana, a teleomorph of Cordyceps bassiana, is a highly efficient producer of mammalian steroids and produces large amounts of sugar-utilizing enzymes. However, the fermentation of steroid glycosides in ginseng with B. bassiana has never been studied. Thus, we evaluated the bioconversion of the major ginsenosides in white ginseng by B. bassiana. Interestingly, B. bassiana increased the total amount of protopanaxadiols and hydrolyzed Rb1 into minor ginsenosides, exhibiting high levels of Rd and Rg3, as well as moderate levels of Rb2 and Rc analyzed by high-performance liquid chromatography coupled with evaporative light-scattering detection. The β-glucosidase activity was highly increased, which led to the selective elimination of sugar moiety at the 20-C position of Rb1 to Rd, followed by Rg3. Rb2 and Rc accumulated because of the minimal activities of α-L-arabinopyranosidase and α-L-arabinofuranosidase, respectively. The fermentation product exerted dose-dependent cytotoxicity in HCT-15 cells, which are resistant to ginseng. The product, but not white ginseng, exhibited apoptotic effects via the Fas ligand and caspase 8/9. This study demonstrates for the first time that the B. bassiana-fermented metabolites have potent apoptotic activity in colon cancer cells, linking to a therapeutic use. PMID:26609787

  19. Application Research on Mould Inhibitors Production of Beauveria bassiana%白僵菌生产中防霉剂的应用研究

    Institute of Scientific and Technical Information of China (English)

    缪东; 舒燕; 邬里洋; 周映华; 王升平; 高书锋; 周小玲; 曾发娇

    2016-01-01

    In order to solve the problem of Beauveria bassiana vulnerable to mould contamination in solid culture, the corn borer Beauveria bassiana ABUV-3 and the pine caterpillars Beauveria bassiana DBUV-7 as for the tested strains, a series of studies of 40%Kejunling and other ifve kinds of mould inhibitors were conducted. The results showed that the ifve kinds of mould inhibitors on spores and bacteria of Mucor, Trichoderma, Rhizopus, green mould and Aspergillus lfavus all have inhibitory effect, in which the inhibitory effects, the 70% of soluble Junjuesha and Lvmeiba powder was the best; although the direct action of the 5 mould inhibitors will bring inhibited effect on the spore germination ofBeauveria bassiana, there was no inhibitory effect on spore production and germination by adding a certain amount of mould inhibitor when producing spore in solid culture. Therefore, it is suggested that in the process of production ofBeauveria bassiana, in solid culture medium added with 500 times liquid of 70% Junjuesha and Lvmeiba powder (equivalent dose for 2 mg / mL) to inhibit mould contamination.%为了解决白僵菌固体培养时易遭受霉菌污染问题,以玉米螟白僵菌ABUV–3和松毛虫白僵菌DBUV–7为供试菌株,对40%克菌灵等5种防霉剂进行了系列研究。结果表明:5种防霉剂对毛霉菌、木霉菌、根霉菌、绿霉菌和黄曲霉菌的孢子和菌体均有抑制作用,其中以70%菌绝杀可溶性和绿霉霸粉剂的抑制效果最好;虽然5种防霉剂直接作用时会对白僵菌的孢子萌发产生抑制作用,但在固体培养生产孢子时添加一定量的防霉剂,对孢子产量及其萌发并没有抑制作用。因此,建议在白僵菌生产过程中,在固体培养基中添加70%菌决杀和绿霉霸粉剂500倍液(折合剂量为2 mg/mL),用以抑制霉菌的污染。

  20. Compatibility of Beauveria bassiana with Neoseiulus barkeri for Control of Frankliniella occidentalis

    Institute of Scientific and Technical Information of China (English)

    WU Sheng-yong; GAO Yu-lin; XU Xue-nong; Mark S Goettel; LEI Zhong-ren

    2015-01-01

    The entomopathogenic fungusBeauveriabassiana (Balsamo) Vuilemin and predatory miteNeoseiulusbarkeri Hughes are effective biological control agents ofFranklinielaoccidentalis Pergande (Thysanoptera: Thripidae), one of the most important pests of ornamentals and vegetables world-wide. Combined application of both may enhance control efifciency. The functional response forN.barkeri on the ifrst instar larvae of western lfower thrips which were infected byB.bassiana for 12 and 24 h in the laboratory ((25±1)°C, (70±5)% RH, L:D=16 h:8 h) was determined. The virulence ofB.bassiana against the second instar and pupae of the thrips attacked byN.barkeri were also tested. The results showed that N.barkeri exhibited a Holing type II functional response on treated thrips. After having been treated with the fungus for 12 h and then offered to the predator, thrips were more vulnerable to be kiled byN.barkeri. The second instar larvae and pupae of the thrips which had been attacked by predatory mites were markedly more susceptible toB.bassiana infection than normal thrips; the cumulative corrected mortality ofB.bassiana of the second instar and pupae which were attacked byN.barkeri were 57 and 94%, respectively, compared to 35 and 80% in controls on the day 8. These ifndings highlight the potential use ofB.bassiana in combination withN.barkeri to controlF. occidentalis.

  1. Estimation of Median Lethal Concentration of Three Isolates of Beauveria bassiana for Control of Megacopta cribraria (Heteroptera: Plataspidae) Bioassayed on Solid Lygus spp. Diet.

    Science.gov (United States)

    Portilla, Maribel; Jones, Walker; Perera, Omaththage; Seiter, Nick; Greene, Jeremy; Luttrell, Randall

    2016-01-01

    The kudzu bug, Megacopta cribraria (F.), is an urban nuisance and significant agricultural pest. The median lethal concentrations of three strains of Beauveria bassiana (Balsamo), including the Mississippi Delta native strain (NI8) isolated from Lygus lineolaris (Palisot de Beauvois), the commercial strain BotaniGard(®) (GHA) (Victor, NY, USA), and the B. bassiana strain isolated from M. cribraria (KUDSC), were estimated on kudzu bug adults. A technique developed to evaluate B. bassiana against L. lineolaris was used. Younger adults (eight days after collection) were treated with NI8 and GHA and older adult (50 days after collection) were treated with NI8, GHA and KUDSC. Higher concentrations (n × 10⁶, n × 10⁷) of NI8 and GHA caused kudzu bug mortality two days after treatment in younger adults and similar concentrations of NI8, GHA, and KUDSC caused mortality one day after treatment in older adults. Lower concentrations (n × 10⁴, n × 10⁵) were not significantly different in mortality between strains. LS50 values of the KUDSC were significantly lower than NI8 and GHA values in older adults. This is the first available information on median lethal concentration of B. bassiana on kudzu bug adults bioassayed on artificial diet. It was determined that B. bassiana (KUDSC and NI8) are highly effective for young adults at very low doses (LC50 1.98-4.98 viable spores per mm²). PMID:27376335

  2. Adulticidal and larvicidal activity of Beauveria bassiana and Metarhizium anisopliae against housefly, Musca domestica (Diptera: Muscidae), in laboratory and simulated field bioassays.

    Science.gov (United States)

    Mishra, Sapna; Kumar, Peeyush; Malik, Anushree; Satya, Santosh

    2011-06-01

    The susceptibility of the adult and larval stage of housefly, Musca domestica L. (Diptera: Muscidae), to two entomopathogenic fungi, Metarhizium anisopliae (Metsch.) Sor. and Beauveria bassiana (Bals.) Vuill., was evaluated under laboratory and simulated field bioassays. Bioassays on adult houseflies were carried out at different conidial concentrations ranging from 10(3) to 10(9) conidia/ml in petri plate and minichamber assays. Absolute mortality was observed within 4-5 days at all the concentrations tested. M. anisopliae was found to be more effective with LC(50) of 6.75 × 10(7) conidia/ml compared with 1.21 × 10(8) conidia/ml of B. bassiana in petri plate bioassay. Similar trend was observed in minichamber bioassay. Larvicidal activity evaluated through petri plate bioassay also indicated that M. anisopliae was more effective larvicide with LC(50) of 4.1 × 10(8) conidia/ml as against 3.31 × 10(9) conidia/ml of B. bassiana. Larvicidal activity was further evaluated in simulated field condition of decaying waste matrix using dry conidial formulations (10(8) conidia/g) of both the fungi. Larval mortality obtained in this assay was 43% (B. bassiana) and 63% (M. anisopliae). Remarkably better performance of M. anisopliae as an adulticidal and larvicidal agent over B. bassiana in laboratory bioassays as well as simulated field conditions suggests that it may have good potential to become part of an integrated housefly control program.

  3. 白僵菌RAPD-PCR反应体系优化%RAPD-PCR Reaction System for Beauveria bassiana

    Institute of Scientific and Technical Information of China (English)

    魏晓鹏; 李会平; 黄大庄; 唐秀光

    2012-01-01

    为了建立一套适宜于白僵菌(Beauveria bassiana)退化研究的RAPD-PCR反应体系及反应程序,通过采用L16( 45)正交试验及退火温度和循环次数的单因素优化对反应体系中的各因素进行优化组合.结果表明:20μL PCR反应体系及反应程序中各因素优化组合为,10×Buffer 2 μL,MgCl2(25 mmol/L)2.4 μL,4种dNTP(各2.5mmol/L)0.8 μL,随机引物(10 μmol/L)1.4 μL,TaqDNA聚合酶(5 U/μL)0.4 μL,模板DNA( 10 mg/L)1 μL.反应条件为,94℃预变性2 min,94℃变性30 s,38℃退火40 s,72℃延伸1 min,循环次数40次,72℃延伸5 min.%An experiment was conducted to establish an optimal PCR (polymerase chain reaction) reaction system and procedure for the degradation of Beauveria bassiana. Single factor test and L16(45) orthogonal experiment were used to optimize the combination of factors for the reaction system. The optimum factor combination was obtained with 20 μL reaction volume containing 2 μL l0×Buffer, 2.4 μL MgCl2(25 mmol/L) , 0. 8 μL four types of dNTPs (each 2. 5 mmol/L) , 1.4μL random primer (10 μmol/L) , 0.4 μL Taq polymerase (5 U/ μL) , and 1 μL template DNA (10 mg/L). Reaction conditions were as follows; predenaturing at 94 degrees C for 2 min, followed by 40 cycles of denaturing at 94 degrees C for 30 s, annealing at 38 degrees C for 40 s, extension at 72 degrees C for 1 min, and final extension at 72 degrees C for 5 min.

  4. Anopheline and culicine mosquitoes are not repelled by surfaces treated with the entomopathogenic fungi Metarhizium anisopliae and Beauveria bassiana

    Directory of Open Access Journals (Sweden)

    Mnyone Ladslaus L

    2010-08-01

    Full Text Available Abstract Background Entomopathogenic fungi, Metarhizium anisopliae and Beauveria bassiana, are promising bio-pesticides for application against adult malaria mosquito vectors. An understanding of the behavioural responses of mosquitoes towards these fungi is necessary to guide development of fungi beyond the 'proof of concept' stage and to design suitable intervention tools. Methods Here we tested whether oil-formulations of the two fungi could be detected and avoided by adult Anopheles gambiae s.s., Anopheles arabiensis and Culex quinquefasciatus. The bioassays used a glass chamber divided into three compartments (each 250 × 250 × 250 mm: release, middle and stimulus compartments. Netting with or without fungus was fitted in front of the stimulus compartment. Mosquitoes were released and the proportion that entered the stimulus compartment was determined and compared between treatments. Treatments were untreated netting (control 1, netting with mineral oil (control 2 and fungal conidia formulated in mineral oil evaluated at three different dosages (2 × 1010, 4 × 1010 and 8 × 1010 conidia m-2. Results Neither fungal strain was repellent as the mean proportion of mosquitoes collected in the stimulus compartment did not differ between experiments with surfaces treated with and without fungus regardless of the fungal isolate and mosquito species tested. Conclusion Our results indicate that mineral-oil formulations of M. anisopliae and B. bassiana were not repellent against the mosquito species tested. Therefore, both fungi are suitable candidates for the further development of tools that aim to control host-seeking or resting mosquitoes using entomopathogenic fungi.

  5. Differential contributions of five ABC transporters to mutidrug resistance, antioxidion and virulence of Beauveria bassiana, an entomopathogenic fungus.

    Science.gov (United States)

    Song, Ting-Ting; Zhao, Jing; Ying, Sheng-Hua; Feng, Ming-Guang

    2013-01-01

    Multidrug resistance (MDR) confers agrochemical compatibility to fungal cells-based mycoinsecticdes but mechanisms involved in MDR remain poorly understood for entomopathogenic fungi, which have been widely applied as biocontrol agents against arthropod pests. Here we characterized the functions of five ATP-binding cassette (ABC) transporters, which were classified to the subfamilies ABC-B (Mdr1), ABC-C (Mrp1) and ABC-G (Pdr1, Pdr2 and Pdr5) and selected from 54 full-size ABC proteins of Beauveria bassiana based on their main domain architecture, membrane topology and transcriptional responses to three antifungal inducers. Disruption of each transporter gene resulted in significant reduction in resistance to four to six of eight fungicides or antifungal drugs tested due to their differences in structure and function. Compared with wild-type and complemented (control) strains, disruption mutants of all the five transporter genes became significantly less tolerant to the oxidants menadione and H₂O₂ based on 22-41% and 10-31% reductions of their effective concentrations required for the suppression of 50% colony growth at 25°C. Under a standardized spray, the killing actions of ΔPdr5 and ΔMrp1 mutants against Spodoptera litura second-instar larvae were delayed by 59% and 33% respectively. However, no significant virulence change was observed in three other delta mutants. Taken together, the examined five ABC transporters contribute differentially to not only the fungal MDR but antioxidant capability, a phenotype rarely associated with ABC efflux pumps in previous reports; at least some of them are required for the full virulence of B. bassiana, thereby affecting the fungal biocontrol potential. Our results indicate that ABC pump-dependent MDR mechanisms exist in entomopathogenic fungi as do in yeasts and human and plant pathogenic fungi.

  6. Differential contributions of five ABC transporters to mutidrug resistance, antioxidion and virulence of Beauveria bassiana, an entomopathogenic fungus.

    Directory of Open Access Journals (Sweden)

    Ting-Ting Song

    Full Text Available Multidrug resistance (MDR confers agrochemical compatibility to fungal cells-based mycoinsecticdes but mechanisms involved in MDR remain poorly understood for entomopathogenic fungi, which have been widely applied as biocontrol agents against arthropod pests. Here we characterized the functions of five ATP-binding cassette (ABC transporters, which were classified to the subfamilies ABC-B (Mdr1, ABC-C (Mrp1 and ABC-G (Pdr1, Pdr2 and Pdr5 and selected from 54 full-size ABC proteins of Beauveria bassiana based on their main domain architecture, membrane topology and transcriptional responses to three antifungal inducers. Disruption of each transporter gene resulted in significant reduction in resistance to four to six of eight fungicides or antifungal drugs tested due to their differences in structure and function. Compared with wild-type and complemented (control strains, disruption mutants of all the five transporter genes became significantly less tolerant to the oxidants menadione and H₂O₂ based on 22-41% and 10-31% reductions of their effective concentrations required for the suppression of 50% colony growth at 25°C. Under a standardized spray, the killing actions of ΔPdr5 and ΔMrp1 mutants against Spodoptera litura second-instar larvae were delayed by 59% and 33% respectively. However, no significant virulence change was observed in three other delta mutants. Taken together, the examined five ABC transporters contribute differentially to not only the fungal MDR but antioxidant capability, a phenotype rarely associated with ABC efflux pumps in previous reports; at least some of them are required for the full virulence of B. bassiana, thereby affecting the fungal biocontrol potential. Our results indicate that ABC pump-dependent MDR mechanisms exist in entomopathogenic fungi as do in yeasts and human and plant pathogenic fungi.

  7. Characterization of T-DNA insertion mutants with decreased virulence in the entomopathogenic fungus Beauveria bassiana JEF-007.

    Science.gov (United States)

    Kim, Sihyeon; Lee, Se Jin; Nai, Yu-Shin; Yu, Jeong Seon; Lee, Mi Rong; Yang, Yi-Ting; Kim, Jae Su

    2016-10-01

    The bean bug, Riptortus pedestris, is a major agricultural pest that reduces crop quality and value. Chemical pesticides have contributed to pest management, but resistance to these chemicals has significantly limited their use. Alternative strategies with different modes of action, such as entomopathogenic fungi, are therefore of great interest. Herein, we explored how entomopathogenic fungi can potentially be used to control the bean bug and focused on identifying virulence-related genes. Beauveria bassiana (JEF isolates) were assayed against bean bugs under laboratory conditions. One isolate, JEF-007, showed >80 % virulence by both spray and contact exposure methods. Agrobacterium tumefaciens-mediated transformation (AtMT) of JEF-007 generated 249 random transformants, two of which (B1-06 and C1-49) showed significantly reduced virulence against Tenebrio molitor and R. pedestris immatures. Both species were used for rapid screening of virulence-reduced mutants. The two transformants had different morphologies, conidial production, and thermotolerance than the wild type. To determine the localization of the randomly inserted T-DNA, thermal asymmetric interlaced (TAIL) PCR was conducted and analysis of the two clones found multiple T-DNA insertions (two in B1-06 and three in C1-49). Genes encoding complex I intermediate-associated protein 30 (CIA30) and the autophagy protein (Atg22) were possibly disrupted by the T-DNA insertion and might be involved in the virulence. This work provides a strong platform for future functional genetic studies of bean bug-pathogenic B. bassiana. The genes putatively involved in fungal virulence should be experimentally validated by knockdown in future studies. PMID:27470140

  8. Efeito de beauveria bassiana (bals. Vuillemin e Metarhizium anisopliae (metsch. sorokin nos parâmetros biológicos de trichogramma atopovirilia oatman & platner, 1983 (hymenoptera: trichogrammatidae Effect of Beauveria bassiana (Bals. Vuillemin and Metarhizium anisopliae (Metsch. Sorokin on the biological parameters of Trichogramma atopovirilia Oatman & Platner (Hymenoptera: Trichogrammatidae

    Directory of Open Access Journals (Sweden)

    Ricardo Antonio Polanczyk

    2010-12-01

    Full Text Available Dois experimentos foram realizados para avaliar o efeito de duas formulações comerciais à base de Beauveria bassiana (Bals. Vuillemin e Metarhizium anisopliae (Metsch., Sorokin sobre os parâmetros biológicos de Trichogramma atopovirilia Oatman & Platner, 1983. No primeiro experimento, cartelas com ovos de Spodoptera frugiperda, 1797 foram mergulhadas em suspensões preparadas com os produtos e, em seguida, colocadas à disposição dos parasitóides para oviposição, durante um período de 24 horas. No segundo, fêmeas do parasitóide foram alimentadas com uma solução mel e suspensão de conídios. Em ambos os experimentos as fêmeas mortas foram colocadas em uma câmara úmida para observar a esporulação dos fungos. Os tratamentos foram mantidos em câmara climatizada com temperatura de 25±1º C, umidade relativa de 70±10% e fotofase de 14 horas. Avaliou-se a longevidade e mortalidade dos adultos, índice de parasitismo, emergência do parasitóide, número de indivíduos por ovo e razão sexual dos descendentes. As formulações não interferiram nos parâmetros avaliados e não foi observada a esporulação do fungo no cadáver do parasitóide adulto. É possível inferir que T. atopovirilia e os fungos entomopatogênicos B. bassiana e M. anisopliae são compatíveis e podem ser empregados simultaneamente em programas de manejo integrado de S. frugiperda.Two bioassays were performed to evaluate the effect of two biopesticides based on Beauveria bassiana (Bals. Vuillemin and Metarhizium anisopliae (Metsch. Sorokin on the biological parameters of Trichogramma atopovirilia Oatman & Platner, 1983. In the first one, displays with S. frugiperda, 1797 eggs were dropped into the biopesticide suspension and offered to the parasitoid females for 24 hours. In the second one, parasitoid females were fed with a suspension containing honey and biopesticide suspension. In both cases, after the parasitoid death they were mantained into a humid

  9. The effect of Mirabilis jalapa leaves biopesticide treatment on the mycelium growth of entomopathogenic fungi Beauveria bassiana inside the larvae body Crocidolomia binotalis

    Science.gov (United States)

    Pramita, Mia; Anggraeni, Tjandra

    2015-09-01

    Pest control with biological method (biopesticide and entomopathogenic fungi) is an alternative program to reduce application of chemical insecticide. Biopesticide of Mirabilis jalapa leaves has been discovered rich in secondary metabolites which has antifeedant activity that can provide physiological interference in insect larvae and the generation numbers[1]. Entomopathogenic fungi Beauveria bassiana has potential to control pest populations[2]. The growth of mycelium B. bassiana may interfere metabolism process inside the host body. Otherwise, B. bassiana produce toxins such as beauvericin that can increase mortality of pest. Combination of M. jalapa and B. bassiana reduce LT50 on C. binotalis larvae[3]. Thus, this study aims to determine influence of provision of biopesticide M. jalapa leaves on growth of mycelium entomopathogenic fungi B. bassiana inside larvae body C. binotalis and to detect the presence of beauvericin in vivo. Third instar larvae of C. binotalis were divided into a control, fungal and combination group. The combination group was given biopesticide and fungi. The concentration of biopesticide was 0.8% (w/v) and concentration of fungi spores was 107 spores/ml. Spores (vol. 5µl) done topically to larvae in interval 6 hours after treatment of biopesticide on non-pesticide cabbage leaves. Afterwards, histological observations performed at 24, 48, 72, 96 hours after treatment. The result show of emergence hyphae and mycelium growth inside lumen of larvae midgut on combination group faster than fungal group. This is thought to be caused by the influence of secondary metabolites of biopesticide M. jalapa leaves. In addition, beauviricin is detectable both of fungal and combination group. Thus, it can be concluded that treatment of biopesticide from M. jalapa leaves can accelerate on growth of mycelium entomopathogenic fungi B. bassiana inside the larvae body C. binotalis and toxic of B. bassiana such as beauvericin was detected on fungal and

  10. The entomopathogenic fungal endophytes Purpureocillium lilacinum (formerly Paecilomyces lilacinus and Beauveria bassiana negatively affect cotton aphid reproduction under both greenhouse and field conditions.

    Directory of Open Access Journals (Sweden)

    Diana Castillo Lopez

    Full Text Available The effects of two entomopathogenic fungal endophytes, Beauveria bassiana and Purpureocillium lilacinum (formerly Paecilomyces lilacinus, were assessed on the reproduction of cotton aphid, Aphis gossypii Glover (Homoptera:Aphididae, through in planta feeding trials. In replicate greenhouse and field trials, cotton plants (Gossypium hirsutum were inoculated as seed treatments with two concentrations of B. bassiana or P. lilacinum conidia. Positive colonization of cotton by the endophytes was confirmed through potato dextrose agar (PDA media plating and PCR analysis. Inoculation and colonization of cotton by either B. bassiana or P. lilacinum negatively affected aphid reproduction over periods of seven and 14 days in a series of greenhouse trials. Field trials were conducted in the summers of 2012 and 2013 in which cotton plants inoculated as seed treatments with B. bassiana and P. lilacinum were exposed to cotton aphids for 14 days. There was a significant overall effect of endophyte treatment on the number of cotton aphids per plant. Plants inoculated with B. bassiana had significantly lower numbers of aphids across both years. The number of aphids on plants inoculated with P. lilacinum exhibited a similar, but non-significant, reduction in numbers relative to control plants. We also tested the pathogenicity of both P. lilacinum and B. bassiana strains used in the experiments against cotton aphids in a survival experiment where 60% and 57% of treated aphids, respectively, died from infection over seven days versus 10% mortality among control insects. Our results demonstrate (i the successful establishment of P. lilacinum and B. bassiana as endophytes in cotton via seed inoculation, (ii subsequent negative effects of the presence of both target endophytes on cotton aphid reproduction using whole plant assays, and (iii that the P. lilacinum strain used is both endophytic and pathogenic to cotton aphids. Our results illustrate the potential of

  11. 玉米根际土球孢白僵菌(Beauveria bassiana)的分离与鉴定%Isolation and Identification of Beauveria bassiana From Maize Rhizosphere Soil

    Institute of Scientific and Technical Information of China (English)

    陈方新; 梅玉云; 张强; 齐永霞; 丁婷

    2016-01-01

    为明确虫生真菌在玉米根际的分布情况,通过选择性培养基对采自安徽省涡阳、萧县、蒙城3地的玉米根际土壤中的虫生真菌进行了分离,并结合形态特征及rDNA ITS序列分析对分离的菌株进行了鉴定.结果表明,从玉米根际土壤中共分离得到39个白僵菌菌株,经鉴定均为球孢白僵菌(Beauveria bassiana),且不同菌株间的菌落生长速度和分生孢子产生量存在显著差异.本研究对丰富玉米地下害虫及土传病害的生物防治资源具有重要的意义.

  12. Optimization of erythritol and glycerol accumulation in conidia of Beauveria bassiana by solid-state fermentation, using response surface methodology.

    Science.gov (United States)

    Tarocco, Federico; Lecuona, Roberto E; Couto, Alicia S; Arcas, Jorge A

    2005-09-01

    Entomopathogenic fungi are widely produced for use as mycoinsecticides. Therefore, improvement of the shelf life of fungal propagules under good and adverse conditions should be a pre-requisite of their production. In order to improve conidial physiology as well as mycoinsecticide efficiency, culture conditions may be varied. The Doehlert design was used to generate response surfaces with an estimation of the parameters of the quadratic model allowing the study of three different factors at a different number of levels. This experimental design was applied to optimize water activity (aw), pH, and fermentation time for Beauveria bassiana conidial production and accumulation of polyols in solid-state fermentation. Thus, it was possible to identify the region in the experimental range in which the optimum values of these parameters were simultaneously achieved. Maximal conidia production was achieved at pH 5-6 and aw=0.999. Under these conditions, polyol accumulation was 3 mg erythritol/g conidia and 29.6 mg glycerol/g conidia. However, maximal polyol accumulation was achieved at pH 4.5 and aw 0.950; erythritol production increased 33-fold and glycerol production 4.5-fold. Under these conditions conidia production was 1,000 times lower. The possibilities of increasing the quality of the biocontrol agent without neglecting yield are discussed. PMID:15731900

  13. Pathogenicity induced by the entomopathogenic fungi Beauveria bassiana and Metarhizium anisopliae in Agrotisipsilon (Hufn.)

    International Nuclear Information System (INIS)

    Scanning electron microscopy (SEM) allowed to observe B. bassiana and M. anisopliae adhesion and penetration structure on A. ipsilon larvae treated with the Lc50 of the fungus, B. bassiana revealed adhesion and penetration structures in the infected larvae. Growth of the fungus on the infected larvae and signs of hyphal penetration of insect cuticle as well as proliferation of the cuticle were also appeared. On the other hand, the fungus, M. anisopliaeas declared by SEM showed a dense network together and caused the green spores on the insect cuticle. Also, SEM allowed observing the spores and hyphae of the fungus in the body cavity of infected larvae. Scanning electron microscopy is convenient tools to observe the mode of action of entomopathogenic fungi and to observe how they are able to colonize and infect the host.

  14. Desenvolvimento dos fungos Metarhizium anisopliae (Metschnikoff, 1879 Sorokin, 1883 E Beauveria bassiana (Balsamo Vuillemin, 1912 sobre Ctenocephalides felis felis (Bouché, 1835 Development of the fungi Metarhizium anisopliae (Metschnikoff, 1879 Sorokin, 1883 and Beauveria bassiana (Balsamo Vuillemin, 1912 on the Ctenophephalides felis felis (Bouché, 1835

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    Denise R. De Melo

    2007-09-01

    Full Text Available A pulga Ctenocephalides felis felis é um parasita causador dermatites alérgicas e também pode transmitir diversos agentes etiológicos aos animais domésticos e aos homens. O objetivo deste trabalho foi verificar o desenvolvimento do fungo sobre a cutícula da pulga, através da microscopia eletrônica de varredura. Os isolados fúngicos testados foram o Metarhizium anisopliae 959 e Beauveria bassiana 986, ambos na concentração 10(8 conídios/ml. Após a exposição dos isolados fúngicos no período de duas, 15, 26 e 96 horas , o material foi processado para a microscopia eletrônica de varredura. Com a obtenção das micrografias, pode-se observar que com 2 horas após exposição aos fungos, os conídios estavam aderidos por toda a cutícula, situando-se preferencialmente nas membranas intersegmentais do abdome. Com 15 horas observou-se a formação do tubo de germinação e a cabeça do apressório e após 26 horas foi possível observar as ramificações e o engrossamento das hifas sobre a cutícula das pulgas. Os resultados indicam que os fungos testados foram capazes de se desenvolver sobre a cutícula de C. f. felis.The flea Ctenocephalides felis felis is a parasite that causes allergic dermatitis and also may transmit etiologic agents to domestic animals and humans. This study investigated by scanning electron microscopy the development of entomopathogenic fungi on flea cuticle. Fleas were exposed to conidia (10(8 ml-1 of Metarhizium anisopliae (isolate 959 or Beauveria bassiana (isolate 986. Following standard protocols for electron microscopy, the specimens were prepared 2, 15, 26 and 96 h after infection. The micrography revealed that 2 h after fungus exposure, conidia attachments encompassed the entire flea cuticle, especially on abdominal intersegmental membranes. The emergence of germ tubes and appressoria formation occurred at 15 h, thickening and branching of hyphae on the flea cuticle was noted at 26 h. Therefore, both of

  15. Ação do fungo Beauveria bassiana, isolado 986, sobre o ciclo biológico do cascudinho Alphitobius diaperinus em laboratório Action of the fungus Beauveria bassiana, strain 986, over the biologic cycle of the Alphitobius diaperinus beetle in laboratory

    Directory of Open Access Journals (Sweden)

    Aleksandro Schafer da Silva

    2006-12-01

    Full Text Available O presente trabalho foi realizado para verificar a eficácia do fungo Beauveria bassiana em todas as etapas de desenvolvimento de Alphitobius diaperinus e assim saber em que fase ele é mais susceptível ao controle biológico. O fungo B. bassiana, isolado 986, foi testado em duas concentrações (3,4 X 10(6 e 3,4 X 10(8 conídios ml-1 mais um grupo controle. Foram realizadas duas leituras, uma sete e outra quatorze dias após a aplicação dos fungos. Na concentração 3,4 X 10(6, 54% dos ovos tratados estavam inférteis, ocorrendo 54% de mortalidade de larvas tratadas nos estádios I, II e III, 22,5% nos estádios IV, V e VI, 9,5% nos estádios VII e VIII e 24,5% de mortalidade das pupas. Na concentração 3,4 X 10(8, 66,8% dos ovos tratados estavam inviáveis, ocorrendo mortalidade de 56% das larvas tratadas nos estádios I, II e III, 34% nos estádios IV, V e VI, 24,5% nos estádios VII e VIII e 49,5% das pupas. Já no grupo controle, 13,3% dos ovos estavam inviáveis, ocorrendo mortalidade de 10% das larvas nos estádios I, II e III, 4% nos estádios IV, V e VI e 0% de mortalidade nos estádios VII, VIII e em pupas. Sobre o cascudinho adulto, o fungo não teve efeito nocivo em nenhum grupo analisado. Com base nesses resultados, observou-se que o fungo nas concentrações testadas apresentou efeito nocivo às fases do ciclo biológico do cascudinho, com exceção do inseto adulto.The present research was carried out to verify the Beauveria bassiana fungus efficacy in all development phases of Alphitobius diaperinus and to know in which phase it is more susceptible for biologic control. The B. bassiana, 986-isolated fungus, was tested in two different concentrations (3.4 x 10(6 and 3.4 x 10(8 conidium ml-1 and a control group. Two readings were realized, one after 7 days of fungus application and the other after 14 days. At concentration of 3.4 x 10(6 conidium ml-1, 54% of treated eggs were infertile, with larva death of 54% at I, II and III

  16. Susceptibilidade de larvas de Cerotoma arcuata Olivier (Coleoptera: Chrysomelidae a Beauveria bassiana (Bals. Vuillemin, Metarhizium anisopliae (Metsch. Sorokin e Bacillus thuringiensis Berliner Susceptibility of Cerotoma arcuata Olivier (Coleoptera: Chrysomelidae larvae to Beauveria bassiana (Bals. Vuillemin, Metarhizium anisopliae (Metsch. Sorokin and Bacillus thuringiensis Berliner

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    Maria Lucia França Teixeira

    2007-02-01

    Full Text Available Larvas de 2° instar de Cerotoma arcuata foram avaliadas em relação à susceptibilidade aos fungos entomopatogênicos Beauveria bassiana, Metarhizium anisopliae e a bactéria Bacillus thuringiensis com as toxinas Cry3. Os insetos adultos foram mantidos em gaiolas e alimentados com plântulas de feijão (Phaseolus vulgaris L. e as larvas em "gerbox" com cotilédones de plântulas de feijão recém-germinadas. Das oito estirpes de B. bassiana avaliadas, CG 156 e CG 213 causaram 100% de mortalidade das larvas, as duas estirpes de M. anisopliae CG 210 e CG 321 foram patogênicas, eliminando 80 e 100% das larvas de C. arcuata, e, das cinco estirpes de B. thuringiensis testadas, o isolado CG 940 causou 70% de mortalidade das larvas.Second instar larvae of Cerotoma arcuata were evaluated concerning the susceptibility to fungi Beauveria bassiana and Metarhizium anisopliae and Bacillus thuringiensis strains containing Cry3 toxin. Adults of C. arcuata were kept in large cages and fed on bean seedlings and the larvae were reared in ‘gearbox’ feeding on germinated Phaseolus bean cotyledons. Strains CG 156 and CG 213 of B. bassiana killed 100% of the insect larvae and strains CG 210 and CG 321 of M. anisopliae killed 80 and 100% of the insect larvae. Strain CG 940 of B. thuringiensis killed 70% of the insect larvae.

  17. Conidia production by Beauveria bassiana (for the biocontrol of a diamondback moth) during solid-state fermentation in a packed-bed bioreactor.

    Science.gov (United States)

    Kang, S W; Lee, S H; Yoon, C S; Kim, S W

    2005-01-01

    Conidia of Beauveria bassiana CS-1, which have the potential for the control of the diamondback moth (Plutella xylostella), were produced by solid-state fermentation (SSF) using a packed-bed bioreactor with rice straw and wheat bran. As the packing density and the bed height were increased, the production of conidia decreased. In a packed-bed bioreactor under no aeration and no addition of polypropylene (PP) foam (control), the total average of conidia was 4.9 x 10(8) g-1. The production of conidia was affected more by the addition of PP foam as an inert support than forced aeration and was approx. 23 times higher than that of the control. The total average of conidia produced by B. bassiana was 1.1-1.2 x 10(10) g-1 .

  18. Conidia production by Beauveria bassiana (for the biocontrol of a diamondback moth) during solid-state fermentation in a packed-bed bioreactor.

    Science.gov (United States)

    Kang, S W; Lee, S H; Yoon, C S; Kim, S W

    2005-01-01

    Conidia of Beauveria bassiana CS-1, which have the potential for the control of the diamondback moth (Plutella xylostella), were produced by solid-state fermentation (SSF) using a packed-bed bioreactor with rice straw and wheat bran. As the packing density and the bed height were increased, the production of conidia decreased. In a packed-bed bioreactor under no aeration and no addition of polypropylene (PP) foam (control), the total average of conidia was 4.9 x 10(8) g-1. The production of conidia was affected more by the addition of PP foam as an inert support than forced aeration and was approx. 23 times higher than that of the control. The total average of conidia produced by B. bassiana was 1.1-1.2 x 10(10) g-1 . PMID:15703878

  19. Seleção de isolados de Metarhizium anisopliae e Beauveria bassiana patogênicos a soldados de Atta bisphaerica e Atta sexdens rubropilosa em condições de laboratório Selection of Metarhizium anisopliae and Beauveria bassiana isolates pathogenic to Atta bisphaerica and Atta sexdens rubropilosa soldiers under laboratory conditions

    Directory of Open Access Journals (Sweden)

    Alzimiro Marcelo Conteiro Castilho

    2010-06-01

    Full Text Available As formigas do gênero Atta são pragas importantes de diversas culturas agrícolas, pastagens e reflorestamentos. Os fungos entomopatogênicos estão entre os fatores naturais de mortalidade dessas formigas e por isso apresentam potencial para serem usados no controle biológico dessa praga. O presente trabalho teve como objetivo selecionar isolados de Metarhizium anisopliae e Beauveria bassiana patogênicos a soldados de Atta bisphaerica e Atta sexdens rubropilosa em condições de laboratório. Para a avaliação da patogenicidade, foram utilizados oito isolados de M. anisopliae e seis de B. bassiana. O experimento foi conduzido em DIC, sendo a parcela composta por um grupo de 10 soldados por espécie de formiga, sendo utilizadas três repetições por tratamento. Para cada isolado, três grupos de soldados foram pulverizados com suspensão de 1,0 x 10(8 conídios ml-1 e mantidos em câmara úmida (25±1°C, 80±1% de UR e no escuro sem alimentação, sendo a mortalidade verificada diariamente. Dos 14 isolados testados, quatro de M. anisopliae e quatro de B. bassiana foram patogênicos aos soldados de ambas as espécies de formigas. A virulência foi avaliada para os isolados que causaram mortalidade igual ou maior a 50%. Para cada isolado, suspensões contendo 1,0 x 10(6 a 1,0 x 10(11 conídios ml-1 foram pulverizadas sobre três grupos de 10 soldados e igualmente acondicionados como no teste de patogenicidade. A porcentagem de mortalidade foi calculada a cada 24 horas para determinação do TL50. O isolado ENA04 de M. anisopliae foi mais patogênico, causando mais de 80% de mortalidade nos primeiros três dias após a inoculação, apresentou maior capacidade de esporular nos cadáveres dos soldados e foi o mais virulento para os soldados de A. bisphaerica, com um TL50 de 1,15 dias. Todos os isolados patogênicos aos soldados de A. sexdens rubropilosa foram igualmente virulentos.The ants of the genus Atta are important pests of several

  20. 白僵菌对烟蚜的致病性研究%Study on Pathogenicity of Beauveria bassiana on Tobacco Aphid

    Institute of Scientific and Technical Information of China (English)

    黄刚; 徐明勇; 钱凤英; 张西仲

    2011-01-01

    [Objective] The aim was to explore the pathogenicity of Beauveria bassiana on tobacco aphid. [ Method] The pathogenicity of four Beauveria bassiana (B-l ,B-2,B-3 and B-4)strains on the tobacco budworm was studied. [ Result] Different tested Beauveria bassiana strains had different levels of insecticidal effect on tobacco aphids. The corrected mortality of tobacco budworm treated by B-3 was more than 95% ; the mortality rates of conidia of B-l and B-2 strains against tobacco aphid were over 80% , and their hyphae also caused higher morbidity a-gainst tobacco aphid; metabolites of different strains of had a strong contact action against tobacco budworm, and all the mortality rates of metabolites of different strains against tobacco aphid were over 85% after 48 h treatment. [Conclusion] The research provides reference for further study on microbial control of tobacco aphid.%[目的]探讨白僵菌(Beauveria bassiana)对烟蚜的致病性.[方法]以4株球孢白僵菌(B-1、B-2、B-3和B-4)为供试菌株,测定其对烟草蚜虫的致病性.[结果]受测的4株白僵菌菌株对烟草蚜虫均有不同程度的杀虫效果.其中,B-3处理烟草蚜虫的校正死亡率达到95%以上,B-1、B-2菌株的分生孢子处理烟草蚜虫的校正死亡率超过80%,其菌丝对蚜虫也有较高的致病率;4株菌株的代谢产物对烟草蚜虫均有较强的触杀作用,施用48 h后其烟草蚜虫的校正死亡率均超过85%.[结论]为烟草蚜虫的微生物防治研究提供了参考.

  1. Pyrethroid resistance in Anopheles gambiae leads to increased susceptibility to the entomopathogenic fungi Metarhizium anisopliae and Beauveria bassiana

    Directory of Open Access Journals (Sweden)

    Knols Bart GJ

    2010-06-01

    Full Text Available Abstract Background Entomopathogenic fungi are being investigated as a new mosquito control tool because insecticide resistance is preventing successful mosquito control in many countries, and new methods are required that can target insecticide-resistant malaria vectors. Although laboratory studies have previously examined the effects of entomopathogenic fungi against adult mosquitoes, most application methods used cannot be readily deployed in the field. Because the fungi are biological organisms it is important to test potential field application methods that will not adversely affect them. The two objectives of this study were to investigate any differences in fungal susceptibility between an insecticide-resistant and insecticide-susceptible strain of Anopheles gambiae sensu stricto, and to test a potential field application method with respect to the viability and virulence of two fungal species Methods Pieces of white polyester netting were dipped in Metarhizium anisopliae ICIPE-30 or Beauveria bassiana IMI391510 mineral oil suspensions. These were kept at 27 ± 1°C, 80 ± 10% RH and the viability of the fungal conidia was recorded at different time points. Tube bioassays were used to infect insecticide-resistant (VKPER and insecticide-susceptible (SKK strains of An. gambiae s.s., and survival analysis was used to determine effects of mosquito strain, fungus species or time since fungal treatment of the net. Results The resistant VKPER strain was significantly more susceptible to fungal infection than the insecticide-susceptible SKK strain. Furthermore, B. bassiana was significantly more virulent than M. anisopliae for both mosquito strains, although this may be linked to the different viabilities of these fungal species. The viability of both fungal species decreased significantly one day after application onto polyester netting when compared to the viability of conidia remaining in suspension. Conclusions The insecticide

  2. The entomopathogenic fungus Beauveria bassiana reduces instantaneous blood feeding in wild multi-insecticide-resistant Culex quinquefasciatus mosquitoes in Benin, West Africa

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    Howard Annabel FV

    2010-09-01

    Full Text Available Abstract Background Mosquito-borne diseases are still a major health risk in many developing countries, and the emergence of multi-insecticide-resistant mosquitoes is threatening the future of vector control. Therefore, new tools that can manage resistant mosquitoes are required. Laboratory studies show that entomopathogenic fungi can kill insecticide-resistant malaria vectors but this needs to be verified in the field. Methods The present study investigated whether these fungi will be effective at infecting, killing and/or modifying the behaviour of wild multi-insecticide-resistant West African mosquitoes. The entomopathogenic fungi Metarhizium anisopliae and Beauveria bassiana were separately applied to white polyester window netting and used in combination with either a permethrin-treated or untreated bednet in an experimental hut trial. Untreated nets were used because we wanted to test the effect of fungus alone and in combination with an insecticide to examine any potential additive or synergistic effects. Results In total, 1125 female mosquitoes were collected during the hut trial, mainly Culex quinquefasciatus Say. Unfortunately, not enough wild Anopheles gambiae Giles were collected to allow the effect the fungi may have on this malaria vector to be analysed. None of the treatment combinations caused significantly increased mortality of Cx. quinquefasciatus when compared to the control hut. The only significant behaviour modification found was a reduction in blood feeding by Cx. quinquefasciatus, caused by the permethrin and B. bassiana treatments, although no additive effect was seen in the B. bassiana and permethrin combination treatment. Beauveria bassiana did not repel blood foraging mosquitoes either in the laboratory or field. Conclusions This is the first time that an entomopathogenic fungus has been shown to reduce blood feeding of wild mosquitoes. This behaviour modification indicates that B. bassiana could potentially be a new

  3. Regulation of a phenylalanine ammonia lyase (BbPAL) by calmodulin in response to environmental changes in the entomopathogenic fungus Beauveria bassiana.

    Science.gov (United States)

    Kim, Jiyoung; Park, Hyesung; Han, Jae-Gu; Oh, Junsang; Choi, Hyung-Kyoon; Kim, Seong Hwan; Sung, Gi-Ho

    2015-11-01

    Phenylalanine ammonia lyase (PAL, E.C. 4.3.1.5) catalyses the deamination of L -phenylalanine to trans-cinnamic acid and ammonia, facilitating a critical step in the phenylpropanoid pathway that produces a variety of secondary metabolites. In this study, we isolated BbPAL gene in the entomopathogenic fungus Beauveria bassiana. According to multiple sequence alignment, homology modelling and in vitro PAL activity, we demonstrated that BbPAL acts as a typical PAL enzyme in B. bassiana. BbPAL interacted with calmodulin (CaM) in vitro and in vivo, indicating that BbPAL is a novel CaM-binding protein. The functional role of CaM in BbPAL action was to negatively regulate the BbPAL activity in B. bassiana. High-performance liquid chromatography analysis revealed that L -phenylalanine was reduced and trans-cinnamic acid was increased in response to the CaM inhibitor W-7. Dark conditions suppressed BbPAL activity in B. bassiana, compared with light. In addition, heat and cold stresses inhibited BbPAL activity in B. bassiana. Interestingly, these negative effects of BbPAL activity by dark, heat and cold conditions were recovered by W-7 treatment, suggesting that the inhibitory mechanism is mediated through stimulation of CaM activity. Therefore, this work suggests that BbPAL plays a role in the phenylpropanoid pathway mediated by environmental stimuli via the CaM signalling pathway.

  4. Endophytic Ability of Different Isolates of Entomopathogenic Fungi Beauveria bassiana (Balsamo) Vuillemin in Stem and Leaf Tissues of Maize (Zea mays L.).

    Science.gov (United States)

    Renuka, S; Ramanujam, B; Poornesha, B

    2016-06-01

    The present study was conducted to examine the ability of six promising indigenous isolates of Beauveria bassiana (NBAII-Bb-5a, 7, 14, 19, 23 and 45) as an endophyte in maize stem and leaf tissues. Maize seedlings (var. Nithyashree) were inoculated with conidial suspensions and were examined for endophytic establishment in leaf and stems at different intervals during 15-90 days after treatment. All six isolates showed colonization in stem and leaf tissues with varying abilities of colonization and persistence. The mean percent colonization ranged from 7.41 to 20.37 % in older stem tissues and 3.70 to 21.29 % in young stem tissues and in leaf, it ranged from 6.46 to 27.78 % in older leaf tissues and 11.11 to 26.85 % in young leaf tissues. Among six isolates tested, Bb-23 isolate recorded the maximum mean colonization in older stem (20.37 %), older leaf (27.78 %) and in young stem (21.29 %). Bb-5a isolate showed maximum mean colonization in young leaf tissues (26.85 %). Persistence of inoculated fungal isolates decreased with increase in age of the plant. No physical symptoms of damage were observed in any of the B. bassiana treated plants. No colonization of B. bassiana was observed in the untreated control maize plants. The results obtained in plating and PCR techniques were similar with regard to the confirmation of endophytic establishment of B. bassiana. This study indicated the possibility of using B. bassiana as an endophyte in maize for management of maize stem borer, Chilo partellus. PMID:27570303

  5. Research on Corn Borer Prevention by Using Beauveria Bassiana Sealing Method%白僵菌封垛防治玉米螟研究

    Institute of Scientific and Technical Information of China (English)

    陈佳广

    2014-01-01

    Corn borer is one of the main pests of maize crops. Using beauveria bassiana sealing method can decrease the quantity of corn borer larva. The article introduces the features and growing condition of corn borer, expounds the theory, methods, effects, considerations and advantages of using beauveria bassiana sealing method for corn borer prevention, provides a reference for the application and extension for the technique.%玉米螟是危害玉米作物的主要害虫之一,利用白僵菌可以有效减少玉米螟幼虫数量。介绍玉米螟的危害特点与发生条件,阐述白僵菌封垛防治玉米螟技术的原理、方法、效果、注意事项及优点,为该技术的推广应用提供参考。

  6. Conclusion on the peer review of the pesticide risk assessment of the active substances Beauveria bassiana strains ATCC-74040 and GHA

    Directory of Open Access Journals (Sweden)

    European Food Safety Authority

    2013-01-01

    Full Text Available The conclusions of the European Food Safety Authority (EFSA following the peer review of the initial risk assessments carried out by the competent authority of the rapporteur Member State Germany, for the pesticide active substances Beauveria bassiana strains ATCC-74040 and GHA are reported. The context of the peer review was that required by Commission Regulation (EC No 2229/2004, as amended by Commission Regulation (EC No 1095/2007 and Commission Regulation (EU No 114/2010. The conclusions were reached on the basis of the evaluation of the representative uses of Beauveria bassiana strains ATCC-74040 and GHA as an insecticide on tomatoes for strain ATCC-74040 and on tomatoes, cucumbers and ornamentals for strain GHA. The reliable endpoints concluded as being appropriate for use in regulatory risk assessment, derived from the available studies and literature in the dossier peer reviewed, are presented. Missing information identified as being required by the regulatory framework is listed. Concerns are identified.

  7. Morphological and Molecular Identification of aBeauveria bassiana Strain%一株球孢白僵菌的形态及分子生物学鉴定

    Institute of Scientific and Technical Information of China (English)

    刘达综; 高慧珊; 刘娜; 何洁明; 徐树兰; 郑常格; 吕思行

    2015-01-01

    利用PDA培养基,对收集自广东茂名的玉米螟(Pyrausta nubilalis)病原真菌(编号:Bba1菌株)进行分离培养。对 Bba1菌株的菌落形态、产孢结构和分生孢子形态、大小进行观察和测量,从形态学初步鉴定该菌株为球孢白僵菌(Beauveria bassiana);同时对Bba1菌株的26,s rDNA和rDNA ITS区域进行序列分析,与NCBI数据库中球孢白僵菌的同源性达到99%,以上。最终判定来自玉米螟的病原真菌Bba1菌株为球孢白僵菌(Beauveria bassiana)。%A strain(No.Bba1)was isolated using PDA culture medium from the muscardine cadaver ofPyrausta nubilalis collected from Maoming in Guangdong Province.In this study,the colony morphology,spore structure,spore shape and size of Bba1 strain were observed and measured,and it was initially identified asBeauveria bassiana.At the same time,its 26,s rDNA and rDNA ITS were sequenced and then compared with those ofBeauveria bassiana in NCBI database.The re-sults showed the homology was above 99%,.Eventually,Bba1 strain was identified asBeauveria bassiana.

  8. Effect of plant extracts and a disinfectant on biological parameters and pathogenicity of the fungus Beauveria bassiana (Bals.) Vuill. (Ascomycota: Cordycipitaceae).

    Science.gov (United States)

    Martins, C C; Alves, L F A; Mamprim, A P

    2016-06-01

    The fungus Beauveria bassiana is naturally found in poultry houses and causes high rates of mortality in Alphitobius diaperinus. Laboratory and field experiments have shown the potential of this fungus as an insect control agent. However, in poultry houses, bacteria as Salmonella, can be found and have been studied alternative control methods for this pathogen. Thus, this study aimed to evaluate the effect of plant extracts and a disinfectant on the fungus Beauveria bassiana (strain Unioeste 4). Conidial viability, colony-forming unit (CFU) counts, vegetative growth, conidia production, insecticidal activity of the fungus and compatibility were used as parameters in the evaluation of the effect of these products on the fungus. Alcoholic and aqueous extracts of jabuticaba (Myrciaria cauliflora (Mart.), guava (Psidium guajava (L.)), and jambolan (Syzygium cumini (L.), at concentrations of 10% as well as the commercial disinfectant, Peroxitane® 1512 AL, were evaluated at the recommended concentrations (RC), 1:200 (RC), 0.5 RC and 2 RC. There was a negative influence of alcoholic and aqueous extracts of jabuticaba, guava and three dilutions of Peroxitane on the viability of conidia. The CFUs and vegetative growth of the fungus were affected only by the Peroxitane (all dilutions). For conidial production, the aqueous extract of guava had a positive effect, increasing production, while the Peroxitane at the R and RC concentrations resulted in a negative influence. The mortality of A. diaperinus, caused by the fungus after exposure to these products, was 60% for the peracetic acid at 0.5 RC, and above 80% for the extracts. Thus, the results showed that all the extracts and Peroxitane at RC 0.5 are compatible with the fungus B. bassiana Unioeste 4, however only the extracts had a low impact on inoculum potential. PMID:27143049

  9. THE INFLUENCE OF ULTRAVIOLET LIGHT ON PATHOGENICITY OF ENTOMOPATHOGENIC FUNGUS BEAUVERIA BASSIANA (BALSAMO VUILLEMIN TO THE EUROPEAN CORN BORER, OSTRINIA NUBILALIS HBN. (LEPIDOPTERA: CRAMBIDAE

    Directory of Open Access Journals (Sweden)

    L Cagán

    2002-05-01

    Full Text Available The influence of different doses of ultraviolet (UV light on the pathogenicity of entomopathogenic fungus Beauveria bassiana (Balsamo Vuillemin to the European corn borer, Ostrinia nubilalis Hbn., and radial growth of fungus was studied in laboratory conditions. The suspensions of B. bassiana isolate SK99 were exposed to UV light. Four different doses of UV light were used in the experiment. The distance between exposed suspensions and UV light source was 0.3 m. Exposure duration was 15, 30, 45 and 60 minutes (as A, B, C and D variants. Control variant SK99 and obtained variants SK99A, SK99B, SK99C and SK99D were cultivated 21 days on Sabourard-dextrose agar. The larvae of O. nubilalis were infected with dry powder consisted of mycelia and spores from fungus cultures. During 10 days, the mortality of infected larvae was evaluated. It was ascertained that UV light exposition significantly influenced the mortality effect of B. bassiana isolates to O. nubilalis larvae. Variant SK99C showed the highest level of infectivity. Radial growth of UV variants was slower with rising time of exposure. The best ability to grow possessed non-irradiated isolate SK99 and the worse variant SK99D. The difference between these two variants was significant.

  10. Up-regulation of carbon metabolism-related glyoxylate cycle and toxin production in Beauveria bassiana JEF-007 during infection of bean bug, Riptortus pedestris (Hemiptera: Alydidae).

    Science.gov (United States)

    Yang, Yi-Ting; Lee, Se Jin; Nai, Yu-Shin; Kim, Sihyeon; Kim, Jae Su

    2016-10-01

    Beauveria bassiana (Bb) is used as an environment-friendly biopesticide. However, the molecular mechanisms of Bb-host interactions are not well understood. Herein, RNA isolated from B. bassiana (Bb JEF-007) and Riptortus pedestris (Hemiptera: Alydidae) infected with this strain were firstly subjected to high-throughput next generation sequencing (NGS) to analyze and compare transcriptomes. Due to lack of fungal and host genome information, fungal transcriptome was processed to partially exclude non-infection specific genes and host-flora. Differentially Expressed Gene (DEG) analysis showed that 2381 genes were up-regulated and 2303 genes were down-regulated upon infection. Most DEGs were classified into the categories of single-organism, cellular and metabolism processes by Gene Ontology analysis. Most DEGs were involved in metabolic pathways based on Kyoto Encyclopedia of Genes and Genomes pathway mapping. Carbon metabolism-related enzymes in the glyoxylate cycle were significantly up-regulated, suggesting a possible role for them in Bb growth in the host. Additionally, transcript levels of several fungal genes were dramatically increased after infection, such as cytotoxic lectin-like protein, bacterial-like toxin, proteins related to cell wall formation, hyphal growth, nutrient uptake, and halogenated compound synthesis. This work provides insight into how entomopathogenic B. bassiana grows in agriculturally harmful bean bug at 6 d post infection. PMID:27647240

  11. Immobilization of Beauveria bassiana Lipase on Silica Gel by Physical Adsorption

    Directory of Open Access Journals (Sweden)

    Vanessa Hitomi Sugahara

    2014-12-01

    Full Text Available Extracellular lipase from Beauveria bassianastrain CG481 was immobilized by using thirteen different immobilization protocols. Silica gel was chosen as the most suitable adsorbent with 94.8% of activity yield. The adsorption on silica gel did not change the optimum pH (8.5 and temperature (45ºC values of the free lipase (FL for lipolytic activity, and it showed higher activities in extreme conditions (pH 9.0 to 10.5, 60ºC. The lipase immobilized on silica gel (ILS showed enhanced stability at pH 7.0 after 120 h incubation (69.0% when compared to FL (33.3%. The thermal stability was also enhanced by immobilization at 60ºC in aqueous (64.6% and organic medium (95.1%, while FL showed only 40.6% of residual activity in aqueous medium and exhibited no activity for esterification reaction in n-heptane. The treatment of ILS with 0.8 M NaCl prevented lipase desorption while Triton X-100 (0.1% resulted the enzyme leakage. The ILS was reused for four times for esterification reaction with 80.8% of initial activity.

  12. 不同宿主的白僵菌菌株分离及特性研究%A study on Beauveria bassiana strains isolated in different hosts and their biological characteristics//

    Institute of Scientific and Technical Information of China (English)

    查玉平; 陈京元; 蔡三山; 赵东容

    2011-01-01

    9 strains of Beaiweria bassiana isolated in different hosts and their biological characteristics were studied. The results showed that there were 8 strains of B. bassiana ( Bals. ) Vuillemin, and a strain of B. brongniartii (Sacc. ) Petch. Based on B. bassiana colony characteristics, vegetative growth and sporulation, the 2nd and 3rd strains were fit for the production.%以9株白僵菌菌株为材料,对其进行分离鉴定并探讨菌株的生物学特性.结果表明:有1株为布氏白僵菌(Beauveria brongniartii),8株为球孢白僵菌(B.bassiana).结合白僵菌菌落特征、营养生长情况和产孢量来看,2号和3号菌株比较适合应用于生产.

  13. Potential of Metarrhizium anisopliae and Beauveria bassiana isolates and Neem oil to control the aphid Lipaphis erysimi (Kalt.) (Hemiptera: Aphididae); Potencial de isolados de Metarhizium anisopliae e Beauveria bassiana e do oleo de Nim no controle do pulgao Lipaphis erysimi (Kalt.) (Hemiptera: Aphididae)

    Energy Technology Data Exchange (ETDEWEB)

    Araujo Junior, Jose M. de; Marques, Edmilson J.; Oliveira, Jose V. de [Universidade Federal Rural de Pernambuco (UFRPE), Recife, PE (Brazil). Dept. de Agronomia - Entomologia], e-mail: jma_junior@yahoo.com.br, e-mail: emar@depa.ufrpe.br, e-mail: vargasoliveira@uol.com.br

    2009-07-15

    This work aimed to determine the efficiency of the entomopathogenic fungi Metarrhizium anisopliae and Beauveria bassiana to control the aphid Lipaphis erysimi (Kalt.) (Hemiptera:Aphididae) in kale Brassica oleracea var acephala D.C., as well as their compatibility with a neem oil formulation (Neemseto{sup R}). Ten isolates of both fungi were tested and the most pathogenic ones were B. bassiana CG001 and M. anisopliae CG30 with 90% and 4.4 days, and 64% and 3.8 days of mortality and median lethal time, respectively. Bioassays with neem at concentrations of 0.5, 1.0 and 2.0% were done either by leaf discs dipping or spraying the aphids on the leaf discs. The neem spraying treatment at 2.0% provided 90% mortality. The use of B. bassiana isolate CG001 or M. anisopliae isolate CG30 with neem at 0.125, 0.25, and 0.5%, demonstrated that these isolates could have their spore viability or colony growth affected when exposed to neem concentrations higher than 0.25%. In absolute values, the isolates B. bassiana CG001 and M. anisopliae CG30 are the most virulent to L. erysimi, and could be utilized in the management of this pest. (author)

  14. Identification of proteases produced by entomopathogenic fungi Beauveria bassiana (Bals) Vuill. strain CG432 previously activated in coffee berry borer alive (Hypothenemus hampei)
    Identificação de proteases produzidas pelo fungo entomopatogênico Beauveria bassiana (Bals) Vuill. Cepa CG432 previamente ativada em insetos vivos de broca do café ((Hypothenemus hampei))

    OpenAIRE

    Luiz Filipe Protásio Pereira; Diogo Maciel de Magalhães; Humberto Josué de Oliveira Ramos; Dalva Trevisan; Eliana Tiemi Ito; Jurandir Pereira Pinto; Vanessa Hitomi Sugahara; Geni Silva Varéa; Jakeliny Akemi Yamamoto Oliveira

    2012-01-01

    Conídios de fungos entomopatogênicos atravessam o exoesqueleto do inseto pela ação mecânica do tubo germinativo e produção de múltiplas isoformas de proteases, quitinases e lipases em resposta à composição da cutícula do inseto. Desta forma o objetivo deste trabalho foi extrair, purificar e caracterizar a estrutura de proteases produzidas em cultivo submerso por Beauveria bassiana CG432 previamente ativada em adultos vivos de broca-do-café (Hypothenemus hampei). Uma suspensão contendo 106 con...

  15. Regulative roles of glutathione reductase and four glutaredoxins in glutathione redox, antioxidant activity, and iron homeostasis of Beauveria bassiana.

    Science.gov (United States)

    Zhang, Long-Bin; Tang, Li; Ying, Sheng-Hua; Feng, Ming-Guang

    2016-07-01

    Multiple glutaredoxins (Grx) and glutathione reductase (Glr) are vital for the thiol-disulfide redox system in budding yeast but generally unexplored in filamentous fungi. Here we characterized the Beauveria bassiana redox system comprising dithiol Grx1, monothiol Grx2-4, Grx-like Grx5, and Glr orthologue. Each grx or glr deletion was compensated by increased transcripts of some other grx genes in normal cultures. Particularly, grx3 compensated the absence of grx1, grx2, grx5, or glr under oxidative stress while its absence was compensated only by undeletable grx4 under normal conditions but by most of other undeleted grx and glr genes in response to menadione. Consequently, the redox state was disturbed in Δglr more than in Δgrx3 but not in Δgrx1/2/5. Superoxide dismutases were more active in normal Δgrx1-3 cultures but less in Δgrx5 or Δglr response to menadione. Total catalase activity increased differentially in all the mutant cultures stressed with or without H2O2 while total peroxidase activity decreased more in the normal or H2O2-stressed culture of Δglr than of Δgrx3. Among the mutants, Δgrx3 showed slightly increased sensitivity to menadione or H2O2; Δglr exhibited greater sensitivity to thiol-oxidizing diamide than thiol-reducing 1-chloro-2,4-dinitrobenzene as well as increased sensitivity to the two oxidants. Intriguingly, all the mutants grew slower in a Fe(3+)-inclusive medium perhaps due to elevated transcripts of two Fe(3+) transporter genes. More or fewer phenotypes linked with biocontrol potential were altered in four deletion mutants excluding Δgrx5. All the changes were restored by targeted gene complementation. Overall, Grx3 played more critical role than other Grx homologues in the Glr-dependent redox system of the fungal entomopathogen. PMID:26969041

  16. 球孢白僵菌液体制剂研究%The Study on A Liquid Preparation of Beauveria Bassiana

    Institute of Scientific and Technical Information of China (English)

    朱天辉; 杨世璋; 李远翔

    2001-01-01

    Effects of different media and fermentative conditions on the production of spores and cumulation of mycelial substence for Beauveria bassiana were studied by means of liquid fermentation. The results show that Beauveria bassiana does not produce blastospores on five media except for the medium (substitute code D) which contains 5% waste sugar, 1% corn abstract, and 0.1% K2HPO4, meanwhile, the D-medium, on which Beauveria bassiana grows well and produces bigger spores, is regarded as a ideal liquid carrier. The optimal fermentative conditions are as follows: temperature 25~30℃, pH6.0~7.0, Vibrating time 96hr, and not excess 1/6 flak volume as ventilation index.%通过液体发酵试验,研究了不同营养基质、不同发酵条件对球孢白僵菌液生分生孢子、芽孢子、干物质积累的影响,结果显示:在5种培养基中,除“糖蜜5%、玉米浸提液1%、K2HPO4 0.1%”(代号D)外,其它均不产生芽孢子,以D配方生物量最高,孢子较大,为本试验选出的液体制剂的理想载体,最佳发酵条件为温度25~30℃,pH6.0~7.0,通气量以瓶装培养液量为指标不超过1/6瓶装量,发酵时间以96小时为佳。白僵菌液体制剂的理想载体与最佳发酵条件的组合可使白僵菌液生分生孢子达2.5×1010左右,超过了目前四川固体发酵的生产水平。

  17. Preparation and Regeneration of Beauveria bassiana (Balsamo) Vuillemin Protoplast%球孢白僵菌原生质体制备和再生条件

    Institute of Scientific and Technical Information of China (English)

    李姝江; 朱天辉; 韩珊; 谯天敏; 闫晓星; 李芳莲; 冯民富

    2011-01-01

    球孢白僵菌[Beauveria bassiana(Balsamo)Vuillemin]经液体培养后,采用酶解方法制备其菌丝原生质体,并采取单因素实验对再生条件进行了研究.结果表明,经纤维素酶制备球孢白僵菌原生质体后,再生的最佳条件是:用L-broth培养基培养球孢白僵菌78 h,KCl 0.8 mol/L(再生用蔗糖0.6 mol/L),柠檬酸-柠檬酸钠缓冲液为缓冲系统,纤维素酶:蜗牛酶:溶菌酶=5:2.5:2.5(mg/mL),在32℃水浴中酶解3 h(pH自然).

  18. Screening of Superior Strain of Beauveria bassiana Parasitized on Monochamus alternatus%松墨天牛寄生白僵菌的优良菌株筛选

    Institute of Scientific and Technical Information of China (English)

    马良进; 杨毅; 张立钦

    2006-01-01

    对21个球孢白僵茵(Beauveria bassiana)茵株的生长、产孢能力、抗逆性等生物学性状以及对松墨天牛(M. alternatus)4龄幼虫的毒力进行了全面的比较研究.综合各菌株的生物学特性和对天牛幼虫的毒力指标,最终得出防治松墨天牛幼虫最优良菌株是来自浙江象山的Bxs和来自江苏的B5菌株,其中Bxs在生长指标上略占优势,B5在抗性指标上略占优势.

  19. Studies on Karyotype of Beauveria bassiana,a Pathogenic Fungus to Bombyx mori L.%家蚕病原白僵菌的核型分析

    Institute of Scientific and Technical Information of China (English)

    时连根

    2000-01-01

    用脉冲凝胶电泳中的等高压均匀电场(counter-clamped homogeneous electric field,CHEF)凝胶电泳技术研究了家蚕病原白僵菌(Beauveria bassiana)核型.家蚕病原白僵菌至少有6条染色体,估算其大小在2.5~7.2Mbp之间,3种分离菌株的核型大小为26.5~29.0Mbp.家蚕病原白僵菌核型在分离菌株间存在多型性.

  20. Advances and Perspectives of the use of the entomopathogenic fungi beauveria bassiana and metarhizium anisopliae for the control of arthropod pests in poultry production

    Directory of Open Access Journals (Sweden)

    DGP Oliveira

    2014-03-01

    Full Text Available Global poultry production is plagued by a wide variety of arthropods. The problems associated with their chemical control have led to an increasing search for control alternatives, and entomopathogenic fungi seem to be a promising strategy. Despite the large number of insects and mites considered as important pests in animal production, studies on the use of entomopathogenic fungi for their control are still scarce compared with agricultural pests, particularly in Brazil. This article reviews some damages and control aspects of the main arthropod pests that affect Brazilian poultry production, including house flies, lesser mealworms, and feather mites, by the use of the entomopathogenic fungi Beauveria bassiana and Metarhizium anisopliae. Studies published in the last 20 years were reviewed, and the main problems and limitations of that pest-control strategy are discussed.

  1. Genetic improvement of the nematicidal fungus Lecanicillium attenuatum against Heterodera glycines by expression of the Beauveria bassiana Cdep1 protease gene.

    Science.gov (United States)

    Xie, Ming; Zhang, Yan-Jun; Zhang, Xiao-Lin; Peng, De-Liang; Yu, Wen-Bin; Li, Qian

    2016-07-01

    Lecanicillium attenuatum is an important nematophagous fungus with potential as a biopesticide against plant-parasitic nematodes. The Pr1A-like cuticle-degrading protease (Cdep1) gene originating from the entomopathogenic fungus Beauveria bassiana was transformed into the nematophagous fungus L. attenuatum using a polyethylene-glycol mediated protoplast-based transformation system. Protease activity was increased 0.64- to 1.63-fold 2-10d after growth in the transformed L. attenuatum. Inhibition of egg-hatching and J2 motility of soybean cyst nematodes (Heterodera glycines) by cell-free fungal culture filtrates were enhanced by 17-76% 2-14d and 43-152% 1-13d after incubation, respectively. PMID:27342597

  2. Using Beauveria bassiana to Control Loudonta dispar%应用白僵菌防治竹镂舟蛾的试验

    Institute of Scientific and Technical Information of China (English)

    谢阿彬

    2011-01-01

    Different generations of Loudonta dispar were conducted controlling experiments by beauveria bassiana with different doses.Result shows that: beauveria bassiana can be used as the excellent pollution-free biological agent to control the 1st 3rd generation of Loudonta dispar in Yidu town of Yongchun county in Fujian province,of which the control effect is optimal;especially for the 1st generation,the correction mortality rate are top to 95.52% after 15 d of control,but the control effect of the 2nd generation is poorer,may be due to the temperature,humidity other factors during the experiment;other agents should be chosen for controlling Loudonta dispar.%采用不同剂量白僵菌对不同代数竹镂舟蛾进行防治试验,结果表明:白僵菌可作为福建省永春县一都镇竹镂舟蛾第1代和第3代的优良无公害生物防治药剂,其防治效果较好,尤其对第1代防治15 d后其校正死亡率最高可达95.52%,但第2代防治效果较差,可能与试验期间温度、湿度等因素影响有关,在防治时应考虑选择其他药剂。

  3. 白僵菌油悬浮剂防治草原蝗虫药效评价%Pesticide Effect Evaluation of Beauveria Bassiana SC on Grassland Locusts

    Institute of Scientific and Technical Information of China (English)

    陈亿兵; 金焕贵; 刘颖

    2012-01-01

    The study was conducted to confirm the practical effect of beauveria bassiana SC on grassland locusts and provide scientific basis for large area application. The results showed that it was safe to steppe vegetation when using the ball spore beauveria bassiana oil SC of 10 billion spores per milliliter in the dosage range from 2 250 to 3 000 mL·hm^-2. The control efficiency on grassland locust was improved by the increasing dosage. The peak effect value after using the drug 7 days,the average control efficiency was 68. 24%-77.44% ,and the aftereffect could last for 7 days. The recommended dosage of the drug was from 2 250 to 3 000 mL·hm^-2 for better use.%为了验证应用白僵菌油悬浮剂防治草原蝗虫的实际效果,为大面积推广应用提供科学依据而进行药效评价试验。结果表明:100亿孢子·mL^-1球孢白僵茼油悬浮剂在2250~3000mL·hm^-2剂量范围内对草原植被生长安全。随着用药量增加,对草原蝗虫的防效也提高。药后7d达到作用高峰,平均防效为68.24%~77.44%,后效可持续7d。推荐用量以2250-3000mL·hm^-2为佳。

  4. Tri-Tek (Petroleum Horticultural Oil and Beauveria bassiana: Use in Eradication Strategies for Bemisia tabaci Mediterranean Species in UK Glasshouses

    Directory of Open Access Journals (Sweden)

    Andrew G. S. Cuthbertson

    2015-02-01

    Full Text Available The sweetpotato whitefly Bemisia tabaci (Gennadius (Hemiptera: Aleyrodidae is a pest of global importance on both outdoor and glasshouse crops. To date, B. tabaci has not become established in the UK. The UK holds Protected Zone status against this pest and, as a result, B. tabaci entering on plant material is subjected to a policy of eradication. Mediterranean species is now the most prevalent Bemisia species entering the UK. Increasing neonicotinoid resistance is becoming increasingly widespread and problematic with this species. As a result, this continues to pose problems for eradication strategies. The current study investigates the efficacy of Tri-Tek (a petroleum horticultural oil awaiting UK registration and the fungus Beauveria bassiana to act as control agents against Mediterranean species in UK glasshouses. Tri-Tek provided 100% egg mortality compared to 74% for B. bassiana. When tested against second instar larvae, mortalities of 69% and 65% respectively were achieved. Both products can be successfully “tank-mixed”. A tank-mix application provided 95.5% mortality of second instar larvae under glasshouse conditions. The potential integration of both products into current Bemisia eradication strategies in UK glasshouses is discussed.

  5. The effect of leaf biopesticide (Mirabilis jalapa) and entomopathogenic fungi (Beauveria bassiana) combinations to some physiological characters and histology of Crocidolomia pavonana (Lepidoptera: Pyralidae) larvae

    International Nuclear Information System (INIS)

    Crocidolomia pavonana is one of the most prominent pest that cause damage to vegetables especially Brassicaceae such us cabbage, broccoli, mustard greens and turnips, these vegetable have been widely consumed and cultivated in Indonesia. The invation of this pest might created high risk of cultivated failure. Enviromentally pest control efforts by utilizing biological control agents such us biopesticides of plants and entomopathogenic fungi have been carried out, but the work was relatively long and strongly influenced by environmental factors. The purpose of this study was to combine biopesticide of Mirabilis jalapa and entomopathogenic fungi Beauveria bassiana to look at mortality of C. pavonana larvae observing by histological incision and scanning electron microscope. Concentration treatments of extracts M. jalapa was (control; 0,1; 0,2; 0,4 and 0,8 gr/ml) and the result showed that the effective concentration was 0,8 g/ml which affect significantly (P<0,05) in reduce pupa weight, improve pupasi time, lowering percentage of emergence imago and improve the long phase of pupa which differ significantly with control. The combination of biopesticides proved to accelerate the mortality of larvae. Histological incision observed at hour 24, 48, 72 and 96, where the biggest damage occurred at hour 96. Observation by scanning electron microscope showed fungus spores that attach to the body surface of larvae subsequently penetrate into the body. Thus the combination use of biopesticides M. jalapa and fungi B. bassiana, can be used as an alternative pest control C. pavonana

  6. The effect of leaf biopesticide (Mirabilis jalapa) and entomopathogenic fungi (Beauveria bassiana) combinations to some physiological characters and histology of Crocidolomia pavonana (Lepidoptera: Pyralidae) larvae

    Energy Technology Data Exchange (ETDEWEB)

    Sirajuddin, Nur Tasmiah, E-mail: nurtasmiah@yahoo.com; Anggraeni, Tjandra, E-mail: nurtasmiah@yahoo.com [Sekolah Ilmu dan Teknologi Hayati - ITB, Jalan Ganesa 10 Bandung (Indonesia)

    2014-03-24

    Crocidolomia pavonana is one of the most prominent pest that cause damage to vegetables especially Brassicaceae such us cabbage, broccoli, mustard greens and turnips, these vegetable have been widely consumed and cultivated in Indonesia. The invation of this pest might created high risk of cultivated failure. Enviromentally pest control efforts by utilizing biological control agents such us biopesticides of plants and entomopathogenic fungi have been carried out, but the work was relatively long and strongly influenced by environmental factors. The purpose of this study was to combine biopesticide of Mirabilis jalapa and entomopathogenic fungi Beauveria bassiana to look at mortality of C. pavonana larvae observing by histological incision and scanning electron microscope. Concentration treatments of extracts M. jalapa was (control; 0,1; 0,2; 0,4 and 0,8 gr/ml) and the result showed that the effective concentration was 0,8 g/ml which affect significantly (P<0,05) in reduce pupa weight, improve pupasi time, lowering percentage of emergence imago and improve the long phase of pupa which differ significantly with control. The combination of biopesticides proved to accelerate the mortality of larvae. Histological incision observed at hour 24, 48, 72 and 96, where the biggest damage occurred at hour 96. Observation by scanning electron microscope showed fungus spores that attach to the body surface of larvae subsequently penetrate into the body. Thus the combination use of biopesticides M. jalapa and fungi B. bassiana, can be used as an alternative pest control C. pavonana.

  7. An entomopathogenic strain of Beauveria bassiana against Frankliniella occidentalis with no detrimental effect on the predatory mite Neoseiulus barkeri: evidence from laboratory bioassay and scanning electron microscopic observation.

    Directory of Open Access Journals (Sweden)

    Shengyong Wu

    Full Text Available Among 28 isolates of Beauveria bassiana tested for virulence against F. occidentalis in laboratory bioassays, we found strain SZ-26 as the most potent, causing 96% mortality in adults at 1×10(7 mL(-1conidia after 4 days. The effect of the strain SZ-26 on survival, longevity and fecundity of the predatory mite Neoseiulus (Amblyseius barkeri Hughes were studied under laboratory conditions. The bioassay results showed that the corrected mortalities were less than 4 and 8% at 10 days following inoculation of the adult and the larvae of the predator, respectively, with 1×10(7 conidia mL(-1 of SZ-26. Furthermore, no fungal hyphae were found in dead predators. The oviposition and postoviposition durations, longevity, and fecundity displayed no significant differences after inoculation with SZ-26 using first-instar larvae of F. occidentalis as prey in comparison with untreated predator. In contrast, the preoviposition durations were significantly longer. Observations with a scanning electron microscope, revealed that many conidia were attached to the cuticles of F. occidentalis at 2 h after treatment with germ tubes oriented toward cuticle at 24 h, penetration of the insect cuticle at 36 h, and finally, fungal colonization of the whole insect body at 60 h. In contrast, we never observed penetration of the predator's cuticle and conidia were shed gradually from the body, further demonstrating that B. bassiana strain SZ-26 show high toxicity against F. occidentalis but no pathogenicity to predatory mite.

  8. Exploiting the genetic diversity of Beauveria bassiana for improving the biological control of the coffee berry borer through the use of strain mixtures.

    Science.gov (United States)

    Cruz, Lina P; Gaitan, Alvaro L; Gongora, Carmenza E

    2006-08-01

    Beauveria bassiana is an entomopathogen widely used to control the coffee berry borer in Colombia, as part of an Integrated Pest Management strategy. Traditionally, the development of fungal insect pathogens as biocontrol agents in crop pests has been oriented towards the selection and formulation of elite clonal strains. Instead, we explored the potential application of genetic diversity in B. bassiana by determining the effect of strain mixtures on coffee berry borer mortality compared to clonal isolates. Genomic DNA from 11 strains was characterized using internal transcribed spacers and beta-tubulin sequences as well as amplified fragment length polymorphism markers. Cluster analysis produced three genetic groups and confirmed the low but significant intraspecific genetic diversity present among the strains. Single strain virulence towards the coffee berry borer under laboratory conditions, using 1x10(6) conidia ml(-1), ranged between 89.9 and 57.5%. All the inoculations with mixtures resulted in coinfection events. Combinations of genetically similar strains showed no significant differences when their virulences were compared. However, mixtures of genetically different strains led to both antagonism and synergism. The lowest virulence percentage (57%) was obtained by putting together the most virulent strain of each group, contrary to the highest virulence percentage (93%) that resulted from mixing the three least virulent strains. The results indicate the promising potential of designing strain mixtures as an alternative for the biocontrol of Hypothenemus hampei and other pests and provide tools for the understanding of the ecological dynamics of entomopathogen populations under natural conditions. PMID:16362818

  9. Serine proteases SP1 and SP13 mediate the melanization response of Asian corn borer, Ostrinia furnacalis, against entomopathogenic fungus Beauveria bassiana.

    Science.gov (United States)

    Chu, Yuan; Liu, Yang; Shen, Dongxu; Hong, Fang; Wang, Guirong; An, Chunju

    2015-06-01

    Exposure to entomopathogenic fungi is one approach for insect pest control. Little is known about the immune interactions between fungus and its insect host. Melanization is a prominent immune response in insects in defending against pathogens such as bacteria and fungi. Clip domain serine proteases in insect plasma have been implicated in the activation of prophenoloxidase, a key enzyme in the melanization. The relationship between host melanization and the infection by a fungus needs to be established. We report here that the injection of entomopathogenic fungus Beauveria bassiana induced both melanin synthesis and phenoloxidase activity in its host insect, the Asian corn borer, Ostrinia furnacalis (Guenée). qRT-PCR analysis showed several distinct patterns of expression of 13 clip-domain serine proteases in response to the challenge of fungi, with seven increased, two decreased, and four unchanged. Of special interest among these clip-domain serine protease genes are SP1 and SP13, the orthologs of Manduca sexta HP6 and PAP1 which are involved in the prophenoloxidase activation pathway. Recombinant O. furnacalis SP1 was found to activate proSP13 and induce the phenoloxidase activity in corn borer plasma. Additionally, SP13 was determined to directly cleave prophenoloxidase and therefore act as the prophenoloxidase activating protease. Our work thus reveals a biochemical mechanism in the melanization in corn borer associated with the challenge by B. bassiana injection. These insights could provide valuable information for better understanding the immune responses of Asian corn borer against B. bassiana. PMID:25900291

  10. Interaction between TATA-Binding Protein (TBP and Multiprotein Bridging Factor-1 (MBF1 from the Filamentous Insect Pathogenic Fungus Beauveria bassiana.

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    Chi Song

    Full Text Available TATA-binding protein (TBP is a ubiquitous component of eukaryotic transcription factors that acts to nucleate assembly and position pre-initiation complexes. Multiprotein bridging factor 1 (MBF1 is thought to interconnect TBP with gene specific transcriptional activators, modulating transcriptional networks in response to specific signal and developmental programs. The insect pathogen, Beauveria bassiana, is a cosmopolitan fungus found in most ecosystems where it acts as an important regulator of insect populations and can form intimate associations with certain plants. In order to gain a better understanding of the function of MBF1 in filamentous fungi, its interaction with TBP was demonstrated. The MBF1 and TBP homologs in B. bassiana were cloned and purified from a heterologous E. coli expression system. Whereas purified BbTBP was shown to be able to bind oligonucleotide sequences containing the TATA-motif (Kd ≈ 1.3 nM including sequences derived from the promoters of the B. bassiana chitinase and protease genes. In contrast, BbMBF1 was unable to bind to these same target sequences. However, the formation of a ternary complex between BbMBF1, BbTBP, and a TATA-containing target DNA sequence was seen in agarose gel electrophoretic mobility shift assays (EMSA. These data indicate that BbMBF1 forms direct interactions with BbTBP, and that the complex is capable of binding to DNA sequences containing TATA-motifs, confirming that BbTBP can link BbMBF1 to target sequences as part of the RNA transcriptional machinery in fungi.

  11. Selection, assessment of virulence to Alphitobius diaperinus, and Pr1 enzyme production of Beauveria bassiana (Bals. Vuill. isolates cultured at stress temperatures

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    Kelly Christiane Constanski

    2015-12-01

    Full Text Available The entomopathogenic fungus Beauveria bassiana is a promising agent for use in insect control. Its pathogenic activity, as well as other factors such as temperature that can interfere with its development, should be assessed, thus, establishing the foundations for B. bassiana use in biological control programs. The objective of this study was to select and induce tolerance of B. bassiana isolates to high and low temperatures and to assess their virulence before and after exposure to those temperatures. A pre-selection test was performed, in which the tolerance of isolates to stress temperatures was tested and compared to the ideal growth temperature of 25 °C for this organism. For the isolates/temperature combinations resulting in growth, conidia germination and colony-forming units (CFUs were assessed. The isolates Unioeste 4 and Unioeste 40 exhibited >95% germinated conidia at 16 and 31 °C. Thereafter, they underwent four consecutive passages at maximum and minimum tolerated temperatures (10 and 37 °C. A significant difference in germination was observed between the two isolates at all temperatures tested. More CFUs were observed for Unioeste 4 compared to Unioeste 40 at all temperatures, and in the case of the latter, there was no difference in CFU formation at 10 and 25 °C. For both isolates, decreased vegetative growth was observed at 37 °C. Recovery of virulence was observed in both isolates, as determined by insect mortality. No relationship was observed between production of the enzyme Pr1 and the virulence of the isolates.

  12. Cloning and expression analysis of a protease maturation factor gene fragment from Beauveria bassiana%球孢白僵菌蛋白酶成熟因子基因片段的克隆及其表达分析

    Institute of Scientific and Technical Information of China (English)

    张军; 谢翎; 孟慧敏; 汪章勋; 黄勃

    2012-01-01

    The fragment of a protease maturation factor gene from Beauveria bassiana was cloned using DDRT-PCR (differential display reverse transcription polymerase chain reaction) . The length of the cloned specific fragment was 295 bp. Analysis of the amino acid sequences indicated that the protease maturation factor from Beauveria bassiana was highly homologous to Aspergillus oryzae (94.44%). The expression of the protease maturation factor gene from Beauveria bassiana under different induced conditions was detected using Realtime PCR. We found that the expression of protease maturation factor gene for cuticle of cicada induced was significantly higher than that in control (nearly 8 times of control). It can be inferred that the protease maturation factor gene in Beauveria bassiana may has important application value in construction of genetic engineering strain with high toxicity.%通过mRNA差异显示技术,从蝉脱诱导培养的球孢白僵菌中分离获得了长度295 bp一种蛋白酶成熟因子的基因片段.该片段编码多肽序列与米曲霉的蛋白酶成熟因子编码氨基酸序列同源性较高,达94.44%.Realtime-PCR检测结果表明,蝉蜕诱导条件下该基因的表达量为对照的8倍左右.诱导下蛋白酶成熟因子的高效表达,表明其在白僵菌毒力相关重组菌株构建研究中可能具有重要的应用价值.

  13. Pathogenicity of Beauveria bassiana to Spodoptera exigua Larvae at Different Temperature and Humidity%不同温湿度下球孢白僵菌对甜菜夜蛾幼虫的致病力

    Institute of Scientific and Technical Information of China (English)

    崔璟辉; 谭周进; 陈浩涛

    2012-01-01

    The pathogenieity of Beauveria bassiana to Spodoptera exigua larvae at different temperature and humidity indoors was studied. The results showed that temperature, relative humidity and larval instar had significant impacts on the pathogenicity of Beauveria bassiana. The temperature range 24 - 27 ℃ , the relative humidity more than 90% and lower larval instar were the most suitable conditions for the infection of Beauveria bassiana to Spodoplera exigua larvae.%在室内研究了球孢白僵菌在不同温度和湿度条件下对甜菜夜蛾幼虫的致病力,结果表明:温度、相对湿度、幼虫龄期对白僵菌的致病力具有显著的影响,24~27℃是球孢白僵菌感染甜菜夜蛾的最适宜温度;相对湿度大于90%,甜菜夜蛾的幼虫虫龄越低,越有利于球孢白僵菌对幼虫的感染.

  14. Molecular tracing of white muscardine in the silkworm, Bombyx mori (Linn.) II. Silkworm white muscardine is not caused by artificial release or natural epizootic of Beauveria bassiana in China.

    Science.gov (United States)

    Chen, Xue; Huang, Cui; He, Lingmin; Zhang, Shengli; Li, Zengzhi

    2015-02-01

    The fungal pathogen Beauveria bassiana causes serious economic losses in sericulture. Its origin is usually attributed to the release of B. bassiana insecticides against pine caterpillars (Dendrolimus punctuatus). In the present study, 488 B. bassiana isolates obtained from silkworm (Bombyx mori) collected from 13 Chinese provinces, and 327 B. bassiana isolates obtained from D. punctatus collected from 9 provinces, were analyzed for population genetic structure using the ISSR technique based on genetic distance. A UPGMA dendrogram clustered them into three independent clades: two B. mori clades and one D. punctatus clade. A 3-D principal component analysis further divided them into two completely independent host groups, revealing high host-specificity. This suggested that white muscardine occurring in B. mori populations throughout southern China was not caused by any B. bassiana strain either naturally prevailing in D. punctatus populations or by any strain artificially released as a fungal insecticide against D. punctatus. We further investigated the genetic differentiation coefficient Gst and gene flow between B. mori-pathogenic and D. punctatus-pathogenic B. bassiana isolates from across China and from five provinces inhabited by both B. mori and D. punctatus. The Gst value across China was computed as 0.410, while the values of the five provinces ranged from 0.508 to 0.689; all above 0.25, which is the threshold for significant genetic differentiation. This suggests that B. bassiana strains isolated from the two different hosts maintained their respective heredity without a convergent homogenization trend, and reduces the possibility that the host range of the caterpillar isolates could expand and enhance their virulence in B. mori. These findings indicate that the use of B. bassiana does not threaten the safety of sericulture. PMID:25541121

  15. Selection of optimum conditions of medium acidity and aeration for submerget cultivation of Bacillus thuringiensis and Beauveria bassiana

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    O. A. Dregval

    2010-06-01

    Full Text Available The paper deals with the influence of medium pH and aeration rate on growth and sporulation of Bacillus thuringiensis and Вeauveria bassiana, which are main constituents of the complex microbial insecticide. It was established optimal medium pH for B. thuringiensis – 6.0 and for В. bassiana – 6.0–7.0. The maximum productivity of the studied microorganisms was observed in the same range of aeration – 7– 14 mmol O2/l/h. The selected conditions of cultivation are necessary for the production of complex biological insecticide based on the association of B. thuringiensis and B. bassiana.

  16. 土壤微生态环境对白僵菌宿存影响的研究%The Effects on Survival Ability of Beauveria bassiana Under Soil Microecological Environment

    Institute of Scientific and Technical Information of China (English)

    李永利; 王圆圆; 董建臻; 周婷; 李静

    2012-01-01

    The aim was to study the effect of soil ecological environment on Beauveria bassiana survival ability. The survival ability of Beauveria bassiana under different soil temperature, soil water content, soil texture and soil microbes was tested. The results showed that soil tempreture: In the 120 days, the survival ability was 5.94×106 cfu/g at -18℃, 1.54×106cfu/g at 25℃, and 8×102 cfu/g at 30℃. Soil water content: survival ability was the hightest on 15% soil water content, and Beauveria bassiana would reduce dramatically lower or higher than 15%; Soil texture: survival ability in loam was significantly higher than sand; soil microorganisms had an impact on the survival ability of Beauveria bassiana, which would promote or restrain the growth of Beauveria bassiana at different stages. The condition of low temperature, loam and 15% water content was conducive to Beauveria bassiana survival ability. Microorganisms restrained Beauveria bassiana survival ability at earlier stage and promoted action at later stage.%为明确土壤微生态环境对白僵菌宿存的影响.测定白僵菌孢子在不同土壤温度、不同土壤含水量、不同土壤质地及土壤微生物存在情况下的宿存量变化.结果表明:土壤温度:120天的宿存期中白僵菌孢子宿存量,-18℃为5.94× 106cfu/g,25℃为1.54× 106 cfu/g,30℃为8× 102 cfu/g;土壤含水量:15%时为3.24× 106 cfu/g,白僵菌的宿存量最高,低于或高于15%都会加速白僵菌宿存量的减少;土壤质地:壤土中白僵菌的宿存量明显高于沙土中的宿存量;土壤微生物的存在能够对白僵菌宿存量产生影响,对白僵菌的生长在不同阶段表现出一定的抑制或促进作用.低温、壤土及土壤含水量为15%的条件下有利于白僵菌的宿存.微生物存在前期对白僵菌宿存有抑制作用,后期表现为促进作用.

  17. Identification of proteases produced by entomopathogenic fungi Beauveria bassiana (Bals Vuill. strain CG432 previously activated in coffee berry borer alive (Hypothenemus hampeiIdentificação de proteases produzidas pelo fungo entomopatogênico Beauveria bassiana (Bals Vuill. Cepa CG432 previamente ativada em insetos vivos de broca do café ((Hypothenemus hampei

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    Luiz Filipe Protásio Pereira

    2012-02-01

    Full Text Available Conídios de fungos entomopatogênicos atravessam o exoesqueleto do inseto pela ação mecânica do tubo germinativo e produção de múltiplas isoformas de proteases, quitinases e lipases em resposta à composição da cutícula do inseto. Desta forma o objetivo deste trabalho foi extrair, purificar e caracterizar a estrutura de proteases produzidas em cultivo submerso por Beauveria bassiana CG432 previamente ativada em adultos vivos de broca-do-café (Hypothenemus hampei. Uma suspensão contendo 106 conídios ativados/mL foi inoculada em meio de cultura líquido a 28ºC, 150 rpm por 3 dias. O extrato de proteases (EP foi obtido da centrifugação a 8000 g por 20 minutos, fracionado e concentrado por ultrafiltração em membrana de porosidade controlada 100 kDa e 3 kDa, respectivamente. A cromatografia de gel filtração em Sephadex G-100 separou um pico proteico (Pico II que apresentou 56% de resíduos do aminoácido ácido aspártico quando analisado por HPLC em coluna de fase reversa ODS-C18; atividade específica 43 vezes superior ao EP sobre soro albumina bovina; atividade de protease tipo-subtilisina e uma única banda proteica revelada por nitrato de prata e Coomassie Brilhant Blue em zimograma sobre gelatina por eletroforese PAGE em condições nativas. A homogeneidade do Pico II foi confirmada pela revelação de uma única banda durante a determinação do pH isoelétrico igual a 4,5, porém a determinação da massa molecular separou 2 bandas de 23 e 26 kDa por eletroforese PAGE-2D. As proteases foram caracterizadas como serino proteases com resíduo cisteína importante para a atividade, pois foram inibidas por fluoreto fenil-metil-sufonil e ácido p-cloromercúriobenzóico. As proteases do Pico II apresentaram Km 4x10-4 sobre substrato tipo-subtilisina.

  18. Efeitos de Beauveria bassiana (Bals Vuill e Metarhizium anisopliae (Metsc Sorok sobre fêmeas ingurgitadas de Amblyomma cajennense (Fabricius, 1787 em condições de laboratório Effects of Beauveria bassiana (Bals Vuill and Metarhizium anisopliae (Metsc Sorok on engorged females of Amblyomma cajennense (Fabricius, 1787 in laboratory conditions

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    R.C.S. Reis

    2004-12-01

    Full Text Available The in vitro susceptibility of Amblyomma cajennense engorged females to some isolated of the fungus Beauveria bassiana and Metarhizium anisopliae was verified and lethal concentrations (LC 50 and LC 90 were calculated. The females were dived in conidia suspensions for five minutes, and kept in climatically controlled chambers BOD under 27° C and 80% relative humidity. Each bioassay had four treatments in concentrations of 10(5,10(6,10(7e10(8 conidia/ml. A control group was also used. The following characteristics were evaluated: weight and period of oviposition, indexes of reproductive and nutritional efficiency and percentage of microbiological control. A dose dependent negative effect was observed in ticks treated with the suspension. All isolates tested cause a negative effect on in vitro tests of engorged females of A. cajennense, suggesting its potential for microbiological control of tick's species.

  19. Targeting of insect epicuticular lipids by the entomopathogenic fungus Beauveria bassiana: hydrocarbon oxidation within the context of a host-pathogen interaction

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    Nicolas ePedrini

    2013-02-01

    Full Text Available Broad host range entomopathogenic fungi such as Beauveria bassiana attack insect hosts via attachment to cuticular substrata and the production of enzymes for the degradation and penetration of insect cuticle. The outermost epicuticular layer consists of a complex mixture of non-polar lipids including hydrocarbons, fatty acids, and wax esters. Long chain hydrocarbons are major components of the outer waxy layer of diverse insect species, where they serve to protect against desiccation and microbial parasites, and as recognition molecules or as a platform for semiochemicals. Insect pathogenic fungi have evolved mechanisms for overcoming this barrier, likely with sets of lipid degrading enzymes with overlapping substrate specificities.. Alkanes and fatty acids are substrates for a specific subset of fungal cytochrome P450 monooxygenases involved in insect hydrocarbon degradation. These enzymes activate alkanes by terminal oxidation to alcohols, which are further oxidized by alcohol and aldehyde dehydrogenases, whose products can enter β-oxidation pathways. B. bassiana contains at least 83 genes coding for cytochrome P450s (CYP, a subset of which are involved in hydrocarbon oxidation, and several of which represent new CYP subfamilies/families. Expression data indicated differential induction by alkanes and insect lipids and 4 CYP proteins have been partially characterized after heterologous expression in yeast. Gene knockouts revealed a phenotype for only one (cyp52X1 out of 6 genes examined to date. CYP52X1 oxidizes long chain fatty acids and participates in the degradation of specific epicuticular lipid components needed for breaching the insect waxy layer. Examining the hydrocarbon oxidizing CYP repertoire of pathogens involved in insect epicuticle degradation can lead to the characterization of enzymes with novel substrate specificities. Pathogen targeting may also represent an important co-evolutionary process regarding insect cuticular

  20. Biological Control of the Chagas Disease Vector Triatoma infestans with the Entomopathogenic Fungus Beauveria bassiana Combined with an Aggregation Cue: Field, Laboratory and Mathematical Modeling Assessment.

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    Lucas Forlani

    2015-05-01

    Full Text Available Current Chagas disease vector control strategies, based on chemical insecticide spraying, are growingly threatened by the emergence of pyrethroid-resistant Triatoma infestans populations in the Gran Chaco region of South America.We have already shown that the entomopathogenic fungus Beauveria bassiana has the ability to breach the insect cuticle and is effective both against pyrethroid-susceptible and pyrethroid-resistant T. infestans, in laboratory as well as field assays. It is also known that T. infestans cuticle lipids play a major role as contact aggregation pheromones. We estimated the effectiveness of pheromone-based infection boxes containing B. bassiana spores to kill indoor bugs, and its effect on the vector population dynamics. Laboratory assays were performed to estimate the effect of fungal infection on female reproductive parameters. The effect of insect exuviae as an aggregation signal in the performance of the infection boxes was estimated both in the laboratory and in the field. We developed a stage-specific matrix model of T. infestans to describe the fungal infection effects on insect population dynamics, and to analyze the performance of the biopesticide device in vector biological control.The pheromone-containing infective box is a promising new tool against indoor populations of this Chagas disease vector, with the number of boxes per house being the main driver of the reduction of the total domestic bug population. This ecologically safe approach is the first proven alternative to chemical insecticides in the control of T. infestans. The advantageous reduction in vector population by delayed-action fungal biopesticides in a contained environment is here shown supported by mathematical modeling.

  1. Efeito de Beauveria bassiana (Bals. Vuill. e Metarhizium anisopliae (Metsch. Sorok. sobre características biológicas de Diatraea saccharalis F. (Lepidoptera: Crambidae = Effect of Beauveria bassiana (Bals. Vuill. and Metarhizium anisopliae (Metsch. Sorok. on Biological Characteristics of Diatraea saccharalis F. (Lepidoptera: Crambidae

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    Marco Aurélio Paes de Oliveira

    2008-04-01

    Full Text Available A broca da cana-de-açúcar Diatraea saccharalis F. é considerada uma das principais pragas nas Américas. Entre os métodos de controle, o uso de fungos entomopatogênicos tem sido amplamente recomendado no manejo das pragas da cana-de-açúcar, incluindo outras lepidobrocas. Assim sendo, este estudo investigou os efeitos de diferentes concentrações de Beauveria bassiana (Bals. Vuill. e Metarhizium anisopliae (Metsch. Sorok. sobre parâmetros biológicos da broca da cana-de-açúcar. Larvas de terceiro instar de D. saccharalis foram tratadas com os fungos usando as concentrações de 103, 104 e 105 conídios mL-1. Larvas tratadas com 105 conídios mL-1 de B. bassiana tiveram menor sobrevivência (56,6%, comparadas com lagartas não-tratadas (90%. Adultos originados de larvas tratadas colocaram menor número de ovos, com menor viabilidade, e viveram menos, comparados com adultos originados de larvas não-tratadas. Larvas tratadas com M. anisopliae na concentração de 105 conídios mL-1 e adultos originados destas larvas também exibiram redução no desempenho, comparados aos insetos não-tratados. Os resultados indicam que B. bassiana e M. anisopliae, além de patogênicos àslarvas de D. saccharalis, também interferem negativamente na sua biologia, mostrando potencial de uso contra esta praga.The sugarcane borer Diatraea saccharalis F. is considered oneof the major sugarcane pests in the American continent. Among control methods, the use of entomopathogenic fungi has been broadly recommended to manage sugarcane pests, including other sugarcane borers. Therefore, this study investigated the effects of differentconcentrations of Beauveria bassiana (Bals. Vuill and Metarhizium anisopliae (Metch. Sorok on biological characteristics of the sugarcane borer. Third-instar larvae of D. saccharalis werefungi-treated using the concentrations of 103, 104 and 105 conidia mL-1. Larvae treated with 105 conidia mL-1 of B. bassiana showed lower survival

  2. An Overview of Application Study of Beauveria bassiana in the Control of Cosmopolites sordidus%球孢白僵菌在香蕉象甲防治中的应用研究概述

    Institute of Scientific and Technical Information of China (English)

    李嘉; 王小奇

    2015-01-01

    The banana weevil Cosmopolites sordidus is the most devastating insect pest of banana crops (Musa spp.), so how to control it effectively has been an important issue. The fungus Beauveria bassiana exhibits good application effects in the control of C. sordidus, and the major achievements are shown in this paper. This will provide a reference for further study on B. bassiana and other entomopathogenic fungi, and their application in pest control.%香蕉象甲(Cosmopolites sordidus)是芭蕉属(Musa spp.)经济作物最具破坏力的害虫,如何对其进行有效防治已成为一项重要议题。球孢白僵菌(Beauveria bassiana)在香蕉象甲防治方面具有很好的应用效果,对其应用研究的主要成果进行概述,这对以后进一步研究球孢白僵菌等昆虫病原真菌及其在害虫防治上的应用具有重要的参考价值。

  3. Survival Rate and Transcriptional Response upon Infection with the Generalist Parasite Beauveria bassiana in a World-Wide Sample of Drosophila melanogaster.

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    Francesco Paparazzo

    Full Text Available The ability to cope with infection by a parasite is one of the major challenges for any host species and is a major driver of evolution. Parasite pressure differs between habitats. It is thought to be higher in tropical regions compared to temporal ones. We infected Drosophila melanogaster from two tropical (Malaysia and Zimbabwe and two temperate populations (the Netherlands and North Carolina with the generalist entomopathogenic fungus Beauveria bassiana to examine if adaptation to local parasite pressures led to differences in resistance. Contrary to previous findings we observed increased survival in temperate populations. This, however, is not due to increased resistance to infection per se, but rather the consequence of a higher general vigor of the temperate populations. We also assessed transcriptional response to infection within these flies eight and 24 hours after infection. Only few genes were induced at the earlier time point, most of which are involved in detoxification. In contrast, we identified more than 4,000 genes that changed their expression state after 24 hours. This response was generally conserved over all populations with only few genes being uniquely regulated in the temperate populations. We furthermore found that the American population was transcriptionally highly diverged from all other populations concerning basal levels of gene expression. This was particularly true for stress and immune response genes, which might be the genetic basis for their elevated vigor.

  4. Effects of Beauveria bassiana on experimental populations of Bactrocera dorsalis%球孢白僵菌对橘小实蝇实验种群的影响

    Institute of Scientific and Technical Information of China (English)

    潘志萍; 翟欣

    2015-01-01

    研究了球孢白僵菌(Beauveria bassiana)B6菌株对橘小实蝇成虫(Bactrocera dorsalis Hendel)的影响.结果表明,在杨桃、番石榴与香蕉3种水果上喷施球孢白僵菌孢子悬浮液,橘小实蝇雌成虫平均产卵量分别为(188.3±11.4)粒、(156.7±13.1)粒、(165.3±9.1)粒,与对照差异不显著;孵化的老熟幼虫数量分别(156.3±11.5)头、(130.3±8.3)头、(147.7±10.6)头,与对照差异也不显著;成虫存活率分别为51.1%、52.2%、62.3%,与对照差异极显著.干扰作用控制指数IIPC为0.554 2、0.573 0、0.667 7,对橘小实蝇成虫控制效果明显.

  5. SELECTION OF BEAUVERIA BASSIANA (BALSAMO VUILLEMIN ISOLATES FOR MANAGEMENT OF MYZUS PERSICAE (SULTZAR (HOM.: APHIDAE BASED ON VIRULENCE AND GROWTH RELATED CHARACTERISTICS

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    Mohammad S. Al-alawi

    2014-01-01

    Full Text Available Isolates of the entomopathogenic fungus Beauveria bassiana originated from Jordan were evaluated for their efficacy against the green peach aphid, Myzus persicae under laboratory and greenhouse conditions. Efficacy evaluation involved testing all isolates at a concentration of 1×107 conidia/mL followed by concentration dependent and greenhouse bioassays for the top virulent isolates. Growth characteristics related to virulence were evaluated for high, intermediate and low virulent isolates. Results showed that three isolates namely: BAU004, BAU018 and BAU019 were highly virulent to the aphid in the laboratory causing more than 75% infection. In the greenhouse, the three isolates caused infection from 41.3 to 46.5%. For the growth characteristics, isolate BAU019 produced more spores than the other highly virulent ones including the commercial isolate GHA. Highly virulent isolates also showed faster hyphal growth than low virulent isolates. These findings indicate that isolates BAU004, Bau018 and BAU019 might be developed as commercial microbial insecticides for safe and effective control of green peach aphid.

  6. 生物防治白僵菌与家蚕病原白僵菌的生物学特性初步比较%A Preliminary Comparison on Biological Characteristics of Biocontrol Agent Beauveria bassiana and Silkworm Pathogen Beauveria bassiana

    Institute of Scientific and Technical Information of China (English)

    刘吉平; 吕思行; 米红霞

    2011-01-01

    In order to provide experimental data for the source-tracking and control of white muscardine disease and for safety assessment of applying Beauveria bassiana insecticide in silkworm rearing areas, PDA medium and scanning electron microscopy were used for isolation, identification and observation of 2 biocontrol agents, namely B. bassiana strain Bb2 used for forest protection in Guangzhou City and B. bassiana strain Bb7 preserved in the South China Agricultural University, and of 3 B. bassiana isolates collected in Guangdong provincial silkworm rearing areas at Huazhou Silkworm Eggs Production Farm, Daqiao Village of Huazhou City, and Xiao Village of Wengyuan County, respectively. A prelimina ry comparison was conducted on the biological characteristics between the above5 strains. The results showed that there existed differences on the colonial morphology, vegetative growth, spore quantity and other biological characteristics of the 5 strains. Among them, strain Bb2 grew the fastest, and strains Bb8 and Bb13 had the highest spore quantities. The conidiospores from muscardine pupae infected by strains Bb8 and Bb14 were spherical or near-spherical under scanning electron microscope. Both of them belonged to [ Beauveria bassiana (Bals.) Vuill. ] The results also showed that there existed certain similarities in biological characteristics between the pathogens of silkworm white muscardine from some silkworm rearing areas and from biocontrol agent B. bassiana. Meanwhile, some diversity also existed in B. bassiana strains from the same silkworm rearing area. Therefore, it is implied that the source of white muscardine is rather complex, and further investigation is thus needed to ascertain whether the two biocontrol agents Bb2 and Bb7 belong to B. bassiana.%对广州市森林保护用生防白僵菌Bb2、华南农业大学保存生防用白僵菌Bb7以及收集自广东省化州蚕种场、化州市笪桥村、翁源县硝村等蚕区的3株家蚕白僵菌分离株(Bb8

  7. Research of Relationship between Conidiation and Virulence of the Beauveria Bassiana to the Caterpillars%球孢白僵菌菌株产孢量及其与毒力关系的研究

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    黄大庆; 姚剑

    2004-01-01

    本研究测定了来源不同的12株球孢白僵菌(Beauveria bassiana)的产孢量及其对马尾松毛虫幼虫的毒力,分析得出相关方程为:LTs0=84508N-0.5,表明菌株对马尾松毛虫的毒力与菌株的产孢量呈正相关关系.

  8. 紫外线诱变选育苜蓿叶象甲高毒力白僵菌%Screening Highly Virulent Strains of Beauveria bassiana to Hypera postica Gyllenhal by UV Mutagenesis

    Institute of Scientific and Technical Information of China (English)

    韩慧; 吴小虎; 李益; 李克梅; 赵莉

    2011-01-01

    [Objective] The purpose of this program was to screen out highly virulent strains of Beauveria bassiana to Hypera postica Cyllenhal. [Method] Ultraviolet irradiating was used to irradiate Beauveria bassiana strain for 10 min and 30 min in order to select high chitinase mutant, and then 3 stage larvae of Hypera postica underwent the virulence test with the Beauveria bassiana before and after UV mutagenesis. [Result]The results of the test indicated that corrected mortality of the stain Bb - 10 - 12 was 100% , corrected mortality of the stain Bb -30- 14 was 77.78%, and corrected mortality of wild type strain was 62.97% in 9 days later. [Conclusion] A more virulent strain Bb - 10 - 12 of Beauveria bassiana to Hypera postica was obtained by UV mutagenesis.%[目的]选育出对苜蓿叶象甲高毒力的白僵菌.[方法]利用紫外线对前期筛选出的白僵菌菌株照射10和30min进行诱变,选出高几丁质酶突变株,再用诱变前后的白僵菌对苜蓿叶象甲3龄幼虫进行毒力测定.[结果]用诱变前后的白僵菌对苜蓿叶象甲3龄幼虫室内毒力测定:处理9d后,诱导菌株Bb- 10- 12校正死亡率为100%,Bb - 30 - 14校正死亡率为77.78%,而朱经紫外线照射的野生型菌株校正死亡率为62.97%.[结论]通过室内毒力测定法,获得了经10 min紫外线照射诱变对苜蓿叶象甲更高毒力的1株白僵菌菌株.

  9. Study on biotransformation products of androstenedione by a Beauveria bassiana strain%一株白僵菌对雄甾烯二酮转化产物的研究

    Institute of Scientific and Technical Information of China (English)

    戈梅; 刘靖; 陈代杰

    2006-01-01

    利用白僵菌Beauveria bassiana HCCB00059对雄甾烯二酮(4AD)进行转化,对其主要产物进行分离纯化和结构鉴定,确认转化产生四个化合物,分别为:11-羟基-睾酮、6,11-羟基睾酮、6,11-羟基-雄甾烯二酮和11-羟基-18-氧杂D扩环雄甾烯二酮.

  10. Study on Application of Beauveria bassiana in Controlling Overwintering Dendrolimus tabulaeformis in North Forest%应用球孢白僵菌防治北方越冬油松毛虫的研究

    Institute of Scientific and Technical Information of China (English)

    李永福

    2011-01-01

    为在北方应用白僵菌防治油松毛虫,笔者采用从灵丘县干旱林区分离得到的油松毛虫病原球孢白僵菌菌株Beauveria bassiana CGMCC NO.1573制备的孢子悬浮液和营养型湿菌粉剂,分别在冬季和春季对越冬的油松毛虫幼虫进行防治。结果显示:用2×106个/mL孢子悬浮液的防治效果不好,校正死亡率只有21%~29%;冬季用菌粉防治效果较好,感染率达到70.4%~85.4%;春季施用菌粉15 d~20 d,感染率达47.1%~54.5%.结论认为,在树干基部和枯落物下撒施"白僵菌营养型湿菌粉剂"集中防治越冬幼虫是北方防治油松毛虫的有效方法。%In order to control pine caterpillar effectively in north forest by using Beauveria bassiana,a strain of Beauveria bassiana CGMCC NO.1573,isolated from Dendrolimus tabulaeformis larva from droughty pine forest of Lingqiu County in Shanxi Province,used as spore suspension preparation and nutrition wet bacteria powder,was employed to the control tests in winter and springtime,respectively.The results showed that the control effect was poor by spraying suspension liquid containing spores of 2×106 numbers per mililiter,and the mortality of the Dendrolimus tabulaeformis larvae was only 21%-29%.While the control effect was very good by using the nutrition wet bacteria powder,the infection rate of overwintering larvae reached 70.4%-85.4%.By comparison,the infection rate reached 47.1%-54.5%,within 15-20 days after using the power preparation in spring.So the conclusion is that it is an effective method by broadcasting the nutrition wet bacteria powder preparation of Beauveria bassiana under the litter layer around the trunk base in the pine forest to control gregarious Dendrolimus tabulaeformis larvae in north China.

  11. Production of Beauveria bassiana with Eupatorium adenophorum%紫茎泽兰生产白僵菌研究

    Institute of Scientific and Technical Information of China (English)

    冯玉元

    2006-01-01

    为使紫茎泽兰Eupatorium adenophorum Spreng这种生长快、蔓延迅速、危害极大的外来杂草变害为宝进行了利用紫茎泽兰生产白僵菌Beauveria bassiana研究.结果表明:用紫茎泽兰草粉替代50%麦麸可生产优质白僵菌,能降低生产成本29%.

  12. Ação do fungo Beauveria bassiana (Balsamo Vuillemin, 1912 sobre a fase parasitária do carrapato Anocentor nitens (Neumann, 1897 Schulze, 1937 (Acari: ixodidae Action of the fungus Beauveria bassiana (Balsamo Vuillemin, 1912 on the parasitic phase of the tick Anocentor nitens (Neumann, 1897 Schulze, 1937 (Acari: ixodidae

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    Silvia Gonzalez Monteiro

    2003-06-01

    Full Text Available A patogenicidade do fungo Beauveria bassiana (isolado 986 foi avaliada no desenvolvimento da fase parasitária do Anocentor nitens, acompanhando o desenvolvimento de larvas não alimentadas até o estágio adulto. Foram utilizados dois tratamentos e um grupo controle com cinco repetições cada. No tratamento 1 (T1, as orelhas dos bovinos foram banhadas com a suspensão fúngica de 10(8 conídios ml-1; no tratamento 2 (T2, as orelhas foram banhadas com água destilada e espalhante adesivo. No grupo controle (C, as orelhas foram banhadas com água destilada. Após 24 horas dos tratamentos, as orelhas de cada animal foram infestadas com 100mg de larvas. Os parâmetros analisados, em cada tratamento, foram: número e peso de fêmeas recuperadas; períodos de pré-postura, postura, incubação, eclosão e longevidade; percentual de eclosão; peso da massa de ovos e da quenógina; índices de eficiência reprodutiva e nutricional. Verificou-se que, no T1 e T2, o número de fêmeas recuperadas, após 35 dias, foi significantemente menor (PThis study evaluated the pathogenicity of the fungus Beauveria bassiana (Isolate 986 on the development of the parasitic phase of Anocentor nitens by accompanying the development of unfed larvae until the adult stage. Two treated and one control group were used with five repetitions each. In the first treated group (T1, cattle ears were bathed with a fungal suspension of 10(8 conidia ml-1, second treated group (T2, the ears were bathed with distilled water and Tween 80 1% and the control group (C, the ears were bathed with distilled water. One day after treatment, the ears of each animal were infested with 100 mg of larvae. The parameters analyzed in each treatment were: number and weight of females recovered; periods of pre-oviposition, oviposition, incubation, eclosion and longevity; percentage of eggs hatched; weight of the egg mass and of females after oviposition. We found that in the two treated groups the

  13. Co-fermentated with Beauveria bassiana and Verticillium lecanii%蜡蚧轮枝菌和球孢白僵菌的共发酵

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    刘锦霞; 杜文静; 李晶; 武建荣; 李娜; 丁品; 张建军

    2012-01-01

    [Objective] Both Beauveria bassiana and Verticillium lecanii are insecticide fungus for biological pest control in the using of study at home and abroad. In order to extend the scope and strengthen the effect of prevention and reduce the cost of biological pest control. Co-fermentation have been applied in this investigation. [Methods] The feasibility of co-fermentation have been studied through comparing sporulation capacity, indoor antibiotic activities of two mixed strains. [Results] Results showed that the best ratio at 1:1 of Verticillium lecanii L-31 and Beauveria bassiana Q-55 by co-fermentation. Taked 10% from every inocu-lated in fermentation medium (Fermentation medium were prepared by yeast extract 5.0 g/L, glucose 20.0 g/L, malt extract 5.0 g/L, KH2PO4 3.0 g/L, millet 200.0 g/L, pH 6.5), 23.0 °C±0.1 °C fermentation temperature 12 d standed the total of spore-containing fermentation broth could reached 1 × 109 CFU/mL or more and have the stronger insecticide toxicity. It had inhibited effect on both Trialeurodes vaporariorum and Pieris rapae L. 9 d after treatment of the lethal concentration LC50 were 2.09×l04±0.12 CFU/mL and 3.17×l05±0.11 CFU/mL, broth concentration 1×108 CFU/mL at the time of death in the LT50 was 2.11±0.14 d and 4.27±0.43 d, correct anti-greenhouse effect in the plot experiment more than 80%, having significant difference with the single strain fermentation control effect. [Conclusion] It provides a scientific basis for further application of the two insecticidal funguses and a reference efficiency way for Spread-spectrum anti-fungal for spread spectrum efficiency of fungal biocontrol agents.%[目的] 球孢白僵菌(Beauveria bassiana)和蜡蚧轮枝茵(Verticillium lecanii)是国内外目前研究应用最广泛的杀虫生防真菌,欲扩大其防治范围、增强防治效果、降低生防成本.[方法] 采用共发酵技术,通过组合菌株产孢能力和杀虫毒力比较试验,确定蜡蚧轮枝茵和球孢白

  14. Toxicity of fifteen strains of Beauveria bassiana against Aphis glycines%球孢白僵菌对大豆蚜毒力的室内生物学测定

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    刘兴磊; 张正坤; 徐文静; 刘影; 王金刚; 李启云

    2011-01-01

    The toxicity of fifteen strains of the entomopathogenic fungus Beauveria bassiana ( Bals. ) Vuill. To Aphis glycines Matsumura was determined. All tested strains were toxic to A. Glycines and obvious differences in the mortality rates achieved by the different strains were positively correlated with spore concentration and treatment time. Strain S14 - X - 1 had the highest amount of toxic isolates.%对来源于吉林省不同地区的15株球孢白僵菌Beauveria bassiana( Bals.) Vuill.菌种对大豆蚜Aphisglycines Matsumura毒力进行了室内生物学测定.所有供试菌株对大豆蚜虫均有一定的致病性,杀虫效率差异明显,杀虫效率与孢子浓度和作用时间均呈正相关.通过对大豆蚜毒力室内生物学测定结果的综合分析,菌株S14-X-1为毒力较高菌株,具有进一步研究应用价值.

  15. 温湿度和幼虫饥饿胁迫对白僵菌毒杀甜菜夜蛾效果的影响%Effects of Temperature, Humidity and Starvation Stress on the Virulence of Beauveria bassiana against the Larvae of Spodoptera exigua

    Institute of Scientific and Technical Information of China (English)

    崔璟辉; 陈浩涛

    2012-01-01

    In this paper, the effects of temperature, humidity and starvation stress on the virulence of Beauveria bassiana against the larvae of Spodoptera exigua in laboratory were studied. The results showed that the temperature and the humidity had significant impact on the virulence of Beauveria bassiana, range of 24-27℃ and humid environment were beneficial to that Beauveria bassiana infecting the larvae of Spodoptera exigua; The starvation stress could shorten the time of Beauveria bassiana killing the larvae of Spodoptera exigua significantly. Therefore, weather factors such as temperature and humidity, food intake of pests, age of the Larvae must be considered when made use of Beauveria bassiana to prevent the larvae of Spodoptera exigua, and the best control effect could be achieved.%为了研究温、湿度和饥饿胁迫对白僵菌防治甜菜夜蛾幼虫的影响,进行了室内试验.结果表明:温度对白僵菌的致病力有显著影响,24~27℃下有利于白僵菌感染甜菜夜蛾幼虫;湿度对白僵菌的致病力也有显著影响,高湿环境有利于白僵菌感染甜菜夜蛾幼虫;饥饿胁迫能显著缩短甜菜夜娥幼虫被白僵菌杀死的时间.因此,在利用白僵菌防治甜菜夜蛾幼虫时,只有充分考虑温、湿度等天气因素,害虫的食物摄取,幼虫虫龄等基本情况,才能取得最佳的防治效果.

  16. Isolation and Identification of highly pathogenic Beauveria bassiana against Thrips%对蓟马类害虫高致病性球孢白僵菌的分离、鉴定

    Institute of Scientific and Technical Information of China (English)

    王俊平; 郑长英

    2011-01-01

    12 different entomopathogenic fungi isolated from diseased Drosicha corpulenta Kuwana showed high virulence with 70%~90% mortality. CYT10 strain was identified based on the morphological and molecular characteristics. Conidiogenous cells were short-globose to flask-shaped and the conidiophore elongated in a long zig-zag extension after each conidium is produced. The result of Blast search indicated that the ITS and EF1-α sequence of CYT10 showed a high similarity with those of other strain of Beauveria bassiana (>95%). Phylogenetic analysis showed that it was closely related to ARSEF-1185 strain, which was belonging to B. Bassiana. CYT10 strain was classified as a member of the species B. Bassiana based on the morphological characteristics and analysis of DNA sequences.%从草履蚧僵虫体内分离出12株病原真菌,对蓟马类昆虫具有70%~90%的致死率.现选出一株病原真菌CYT10利用形态和分子的特点进行鉴定.扫描电镜下观察,该菌产孢细胞簇生瓶状,“之”型产孢轴.经PCR获得rDNA-ITS和EF1 -a核苷酸序列,与其他球孢白僵菌的相应序列一致性达95%以上,进化分析CYT10菌株与球孢白僵菌Beauveria bassiana聚为一类.根据菌株的形态观察和DNA序列分析确定该菌为球孢白僵菌.

  17. 球孢白僵菌对月季长管蚜的生物防治效果%Studies on biological control of Macrosiphum roswomm by using Beauveria bassiana

    Institute of Scientific and Technical Information of China (English)

    姚晓洁; 朱虹

    2015-01-01

    Macrosiphum roswomm is one of the most serious pests on Rosaceae. However, biological control of M. ros⁃womm with Beauveria bassiana still remains unreported. The patho genicity and greenhouse control effect of B. bassiana strain isolated from M.roswomm on aphid nymphs and adults, and the survival of B. bassiana on rose leaves were studied. The results showed that, under the same conditions, aphid mortality increased with increasing spore concentration. At the spore concentration of 108 spores/mL, the highest cumulative mortality was 99�3% in 5 days; the pathogenicity for adults and the third instar nymphs was much higher than the first instar nymph. Under different temperature and humidi⁃ty, the highest mortality was discovered at the condition of 25℃, and the infection mortality rate increased with the in⁃creasing of the environment humidity;the highest infection mortality in greenhouse was 94%. These results indicated that B.bassiana has a good potential in biological control of M. roswomm.%研究了1株分离自月季长管蚜( Macrosiphum roswomm Zhang)的球孢白僵菌( Beauveria bassiana ( Bals.) Vuill)对月季长管蚜若虫和成虫的致病力及温室防治效果,并对球孢白僵菌孢子在月季叶片上的存活动态进行了深入观测。结果表明:相同条件下,月季长管蚜死亡率随球孢白僵菌孢子浓度的增加而上升,在孢子浓度为108 spores/mL时,月季长管蚜5 d的最高累积死亡率为99.3%;球孢白僵菌对成虫和3龄若虫的致病性最强,1龄若虫最低。试验发现25℃时月季长管蚜死亡率最高,环境湿度越大,月季长管蚜的死亡率越高;温室内最高感染死亡率达94%,表明球孢白僵菌在月季长管蚜的生物防治中具有很好的应用潜力。

  18. Pathogenicity of Metarhizium anisopliae (Metch Sorok and Beauveria bassiana (Bals Vuill to adult Phlebotomus duboscqi (Neveu-Lemaire in the laboratory

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    Philip M. Ngumbi, Lucy W. Irungu, Paul N. Ndegwa & Nguya K. Maniania

    2011-03-01

    Full Text Available Background & objectives: Biological control of sandflies using entomopathogenic fungi is a possible alternativeto the expensive synthetic chemical control. It is potentially sustainable, less hazardous, and relatively inexpensiveand merits further investigations. The objective of this study was to identify the most pathogenic fungal isolate(sto sandflies in the laboratory.Methods: Isolates of entomopathogenic fungi Metarhizium anisopliae and Beauveria bassiana were screenedfor their pathogenicity against Phlebotomus duboscqi. Adult flies were contaminated using the technique describedby Migiro et al (2010. Briefly, flies were exposed to 0.1 g of dry conidia evenly spread on a cotton velvet clothcovering the inner side of a cylindrical plastic tube (95 mm long × 48 mm diam. In all 25 sandflies weretransferred into the cylindrical tube and allowed to walk on the velvet for one minute, after which they weretransferred from the velvet into the cages in Perplex. Insects in the control treatments were exposed to fungusfree velvet cloth before being transferred into similar cages. The treatments were maintained at 25 ± 2°C,60–70% RH and 12L: 12D photoperiod. The experiment was replicated 5 times. The most pathogenic isolateswere selected for further studies.Results: A total of 19 isolates were screened against adult sandflies in the laboratory. Mortality in the controlswas approximately 16.8 ± 1.7 %. All the isolates were found to be pathogenic to P. duboscqi. Mortality rangedbetween 76.8 and 100% on all the fungal isolates tested. The lethal time taken to 50% (LT50 and 90% (LT90(mortality ranged from 3.0–7.8 days and from 5.3–16.2 days, respectively. The virulent isolates, causing mortalitiesof 97.5–100%, were selected for further studies.Interpretation & conclusion: The high susceptibility of sandflies to entomopathogenic fungi suggests that fungiare potential alternatives to chemical control methods. We conclude that application of

  19. 响应面法优化球孢白僵菌CXJ-1的发酵工艺条件%Optimizatiom of Fermentation Conditons for Beauveria bassiana CXJ-1 Spore by Response Surface Methodology

    Institute of Scientific and Technical Information of China (English)

    孙佰平; 任娟; 赵思峰; 侯彩霞; 王斌; 刘启鹏

    2011-01-01

    Response surface methodology was used to optimize fermentation conditons in order to improve spore production of Beauveria bassiana CXJ-1. The optimum fermentation temperature, rotation speed and pH were obtained with one-factor-at-time. Through Plackett-Burman experimental design and Box-Behnken experimental design for response surface optimization, the optimal conditions for spore production of Beauveria bassiana CXJ-1 were also determined as follows: temperature 28.07℃ , rotation speed 198. 95 r/min and the pH value 6. 10. Under these conditions, the predicted value of the concentration of spores was 9.5X108 spores/mL and the verified value was 9. 7 X 108 spores/mL, the fermentation validation showed that the experimental value have high inosculating degree to model prediction.%为了提高球孢白僵菌CXJ-1(Beauveria bassiana CXJ-1)在液体发酵过程中分生孢子的产孢量,采用响应面法对球孢白僵菌CXJ-1产孢的发酵工艺条件进行优化.采用单因素实验确定了产孢发酵条件最适初始pH、发酵转速和发酵温度.通过Plackett-Burman实验设计和Box-Behnken实验设计响应面分析优化,确定了球孢白僵菌CXJ-1产孢最佳发酵工艺参数为:温度28.07℃,转速198.95 r/min,初始pH 6.10,预测孢子浓度可达9.5×108个/mL,在优化发酵条件下进行验证实验,孢子浓度达到9.7×108个/mL,表明验证值和预测值有较高的吻合度.

  20. 球孢白僵菌九个菌株对赤拟谷盗成虫的毒效%Efficacy of nine isolates of Beauveria bassiana against adults of Tribolium castaneum (Coleoptera: Tenebrionidae)

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    Hediyeh GOLSHAN; Moosa SABER; Farzad MAJIDI-SHILSAR; Farrokh KARIMI

    2013-01-01

    Beauveria bassiana (Balsamo) Vuillemin is one of the most important entomopathogenic fungi and has been widely used for many insect pests all over the world.In this study,the pathogenicity of nine isolates of B.bassiana was evaluated against adults of Tribolium castaneum (Herbst).Fifteen adults of the pest were submerged into the four suspensions (1 × 106,1 × 107,1 × 10s and 1 × l09 conidia/mL) of different isolates of B.bassiana for 20 s,and the mortality was recorded daily for 14 d.Results showed that IRAN 440C and IRAN 187C isolates had the lowest (5.04 × 107 conidia/mL) and highest (5.05 ×108 conidia/mL) LCs0,and DEBI 005 and DEBI 014 isolates had the shortest (2.88 d) and longest (4.96 d) LT50,respectively.According to the LC5o and LT50 values and the mortality,IRAN 440C is a perfect isolate for using in controlling the pest.%球孢白僵菌Beauveria bassiana (Balsamo) Vuillemin是最重要的昆虫病原真菌,广泛用于防治世界各地的多种害虫.本研究评价了球孢白僵菌9个菌株对赤拟谷盗Tribolium castaneum (Herbst)成虫的致病性.将15头赤拟谷盗成虫浸入到4个浓度(1×106,l×107,l×lo8和l×109个分生孢子/mL)的白僵菌菌株中20 s,14 d内每日记录成虫的死亡率.结果表明:IRAN 440C菌株对赤拟谷盗成虫的LC50最低(5.04 ×l07个分生孢子/mL),IRAN187C菌株的最高(5.05 ×lo8个分生孢子/mL);DEBI 005菌株对赤拟谷盗成虫的LT5o最短(2.88 d),DEBI 014菌株的最长(4.96 d).根据LC50,LT50和死亡率结果得出IRAN 440C是防治这一害虫的理想菌株.

  1. Advanced in the research of conidium preparation additives and formulations of beauveria bassiana%球孢白僵菌分生孢子制剂助剂及剂型研究进展

    Institute of Scientific and Technical Information of China (English)

    朱明媛; 田丽

    2012-01-01

    球孢白僵菌(Beauveria bassiana (Balsamo) Vuillemin)是一种经典的丝孢类昆虫病原真菌,对多种农林害虫有防治作用,其制剂的开发早已成为各国的研究热点.随着新型表面活性剂的出现,粒状、水基性、多功能性的剂型因其省力、安全以及对环境污染较轻等特点越来越受关注.本文就球孢白僵菌制剂助剂及剂型做一综述.

  2. 白僵菌抗多菌灵突变株的诱变及生物学特性研究%INDUCTION AND BIOLOGICAL CHARACTERISTICS OF CARBENDAZIM- RESISTANT MUTANTS OF Beauveria bassiana

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    齐永霞; 陈方新; 李增智

    2012-01-01

    The sensitivity and concentration completely inhibited (MIC) of Beauveria bassiana to carbendazim were studied by means of mycelial growth rate method. The results showed that among the tested strains of Beauveria bassiana, Bb06 was the most sensitive to carbendazim, the MIC of Beauveria bassiana to carbendazim was 9μg/ml. The resistant level of the carbendazim-resistant strains of Beauveria bassiana which induced by mycelium and conidia were determined. The results showed that the resistant level of BC-4 was the highest among the carbendazim-resistant strains, and the EC50 and the resistant level were 258. 7711 and 242.11, respectively. The resistance level of BC-3 was the lowest among the carbendazim-resistant strains, and the ECso was 18.6311. The mycelia growth rate and conidia productivity of the carbendazim-resistant strains were also studied. The results showed that the mycelium growth rate of parent strain (Bb06) was the largest(1.78mm/d) and BC-8 was the smallest(1.41mm/d). The conidia productivity of the carbendazim-resistant strains was stronger than the parent strain Bb06. Among the carbendazim-resistant strains, the conidia productivity of BC-4 was the best.%采用菌丝生长速率法测定了球孢白僵菌对多菌灵的敏感性和最低完全抑制浓度(MIC)。结果显示,在供试的球孢白僵菌菌株中,Bb06菌株对多菌灵最敏感,其EC50值为1.0688μg/ml,多菌灵对球孢白僵菌菌丝生长的MIC为9μg/ml。通过菌丝块诱变法和分生孢子诱变法获得球孢白僵菌抗多菌灵突变株,并测定球孢白僵菌不同抗性突变株对多菌灵的抗性水平。结果表明,在抗性突变株中,BC-4菌株的EC50值最大,为258.7711,对多菌灵的抗性水平最高,达到242.11;BC-3菌株的EC50值最小,为18.6311,抗性水平最低。同时对球孢白僵菌抗多菌灵突变株的菌丝生长速率和产孢能力差异的研究表明,Bb06菌株(亲本菌株)的生长速率最大,为1.78mm

  3. 球孢白僵菌硝酸还原酶基因同源重组载体的构建%Construction of Homologus Recombination Vector for Beauveria bassiana Nitrate Reductase

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    赵曦; 张正坤; 孙召朋; 徐文静; 杜茜; 隋丽; 李启云

    2010-01-01

    根据已报道球孢白僵菌(Beauveria bassiana)硝酸还原酶基因(nitrate reductase,NR)序列设计引物,从球孢白僵菌D1-5菌株基因组DNA中扩增并克隆该基因上下游片段NR1和NR2,成功构建了球孢白僵菌NR基因同源重组敲除载体,以期敲除球孢白僵菌的NR基因,建立白僵菌同源重组基因敲除体系.

  4. Toxicity Determination of Beauveria bassiana to Different Instars of Cotton Aphids%球孢白僵菌对不同龄期棉蚜的毒力测定

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    魏萍; 王恒玺

    2014-01-01

    The toxicity of Beauveria bassiana strain XW0107001 to different instars of cotton aphids was determined by the experiments in laboratory.The results showed that the toxicity was obviously different to dif-ferent instars of cotton aphids and enhanced with the increase of instar.%通过室内试验,测定了球孢白僵菌菌株XW0107001对不同龄期棉蚜的毒力。结果表明:同一球孢白僵菌菌株对不同龄期棉蚜的毒力明显不同,随着龄期增加,球孢白僵菌对其毒力越来越大。

  5. Pathogenicity of Beauveria bassiana to Ephesia elutella and Lasioderma serricorne%白僵菌对烟草粉螟及烟草甲的致病性研究

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    刘爱英; 罗力; 邹晓; 胡海燕; 张晓敏; 杨辉; 吴让新

    2009-01-01

    为进一步研究白僵菌对烟叶仓储害虫的控制作用,将4×1010个孢子/g白僵菌(Beauveria bassiana)孢子粉喷施于有害虫的烟叶上,几天后在5 m距离处设置的空白检测箱中亦检测到感染真菌而死亡的储烟害虫.表明,感染带菌的害虫能自由迁飞.经显微镜检结果证实,白僵菌粉对烟草粉螟和烟草甲均具有较强的致病力,在处理21 d后死亡率分别达31%和84.4%.

  6. Isolation and Identification of Pathogenic Fungi-Beauveria bassiana%沙棘木蠹蛾幼虫病原真菌——球孢白僵菌的分离与鉴定

    Institute of Scientific and Technical Information of China (English)

    王素英; 特木钦; 王英凯; 陆群; 时亚琴; 杨奋勇; 苏梅

    2007-01-01

    在对沙棘木蠹蛾危害情况进行调查时,从死亡的沙棘树干内发现了罹病致死的沙棘木蠹蛾,通过对虫尸体内病原真菌的分离与鉴定,确定为球孢白僵菌(Beauveria bassiana).经室内致病力测定,接种浓度4亿/mL、8亿/mL、12亿/mL,54 d的感病率分别是72.22%、83.33%、100%,证明球孢白僵菌是沙棘木蠹蛾的病原菌.同时表明浓度越高感病率越高.

  7. Caracterización molecular de 15 aislamientos de Beauveria bassiana asociados con Cosmopolites y Metamasius en plátano y banano en tres regiones de Colombia Molecular characterization of 15 isolations of Beauveria bassiana related to Cosmopolites and Metamasius in plantain and banana in three regions of Colombia

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    Diego Fernando Marmolejo

    2008-10-01

    Full Text Available Se colectaron picudos de Cosmopolites y Metamasius en municipios del Valle del Cauca, Caldas y Quindío. Se obtuvieron cultivos monospóricos con diluciones de 10-10 y 10-11. Los aislamientos fueron almacenados a -80°C con glicerol al 10% y el ADN a -20°C. Los marcadores moleculares RAM generaron 82 fragmentos de los cuales 67% fueron polimórficos con una heterocigocidad de 0.24, que indica diversidad media a alta. A un índice de similitud 0.84 se formaron 5 grupos: uno con 11 aislamientos y 4 con un solo aislamiento. En el gran grupo se detectó un duplicado y se encontró diversidad del hongo en los sitios muestreados. No se encontró relación entre aislamientos sobre Cosmopolites y Metamasius o zona geográfica en la formación de grupos genéticos.Weevils Cosmopolites and Metamasius in municipalities of the Valle del Cauca, Caldas and Quindío departaments of Colombia were collected. Monosporic cultures were obtained from10-10 and 10-11 dilutions of Beauveria bassiana. Isolates were kept at -80°C with 10% glycerol and DNA stored at -20°C. RAMs molecular markers generated a total of 82 fragments of which 67% were polymorphic. A heterozygosity value of 0.24 indicated a medium - high diversity. Five groups were formed which have a similarity value of 0.84 and one big group with 11 isolates and four groups with only one isolate. In the big group was detected a duplicate and fungi genetic diversity in the sampled places. Neither relationship among isolates of Cosmopolites and Metamasius nor geographical zone related to the formation of genetic groups.

  8. Virulence Determination of Beauveria bassiana Bb070817 against Bactrocera correcta (Bezzi) in Laboratory%球孢白僵菌Bb070817菌株对番石榴实蝇的室内毒力

    Institute of Scientific and Technical Information of China (English)

    袁盛勇; 孔琼; 李珣; 田学军; 陈斌

    2013-01-01

    为研究球孢白僵菌(Beauveria bassiana)Bb070817菌株对番石榴实蝇Bactrocera correcta(Bezzi)的毒力效果,采用喷雾法和浸渍法以不同密度球孢白偶菌(Beauveria bassiana)Bb070817菌株的分生孢子液处理番石榴实蝇Bactrocera correcta(Bezzi)的成虫、幼虫和蛹,并分别测定对成虫、幼虫和蛹的室内毒力.结果表明,成虫的累积校正死亡率最高,为86.12%,其致死中时间(LT50)为4.66d,致死中密度(LCT50)为2.948 5×105 mL-1.幼虫的累积校正死亡率为88.42%,其LT50为4.47 d,LCT50为2.769 5×105 mL-1.蛹的累积校正死亡率是83.91%,其LT50为5.16d,LCT50为3.155 7×105 mL-1,说明该菌株对番石榴实蝇具有较强的毒力.

  9. Réponse des stades larvaires de Helicoverpa armigera (Hübner (Lepidoptera : Noctuidae à l'application de champignons entomopathogènes Metarhizium anisopliae et Beauveria bassiana

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    Tamò, M.

    2012-01-01

    Full Text Available Response of the nymphs of Helicoverpa armigera (Hübner (Lepidoptera: Noctuidae to entomopathogenic fungi Metarhizium anisopliae and Beauveria bassiana. Two experiments on dose/mortality response between the instars of Helicoverpa armigera and two strains of entomopathogenic fungi, Metarhizium anisopliae (Met 31 and Beauveria bassiana (Bb 11 were carried out in laboratory conditions. In the first experiment, M. anisopliae Met 31 was tested on the third instar of H. armigera, while in the second experiment, both Met 31 and Bb 11 were tested on the fourth instar. In all the experiments, the following different doses of conidia per insect were used: 104, 105, 106, 107. The following parameters were measured: mortality and sporulation rates, the number of pupae formed and the number of adults that emerged. Abbott's formula was used to correct the treatment mortality rates. LD50 was determined using Cox-regression. For the third instar in experiment one, no significant difference was observed between high doses (106 and 107 conidia per insect. For instar L4, only the dose of 107 conidia per insect showed high mortality rates (74%. For the strain Bb 11, in spite of the variation observed between the mortality rates induced by high doses (106 and 107 conidia per insect, no significant difference was recorded at the 5% level. No mycosis was observed from cadavers resulting from lower doses when tested on L4. The control recorded the highest numbers of pupae and adults. These two parameters were related to the level of dosage: the higher the dose, the lower the numbers of pupae and adults that emerged. For all the strains of fungi used, whatever the larval stage of H. armigera, the dose/mortality response was significant.

  10. 银杏大蚕蛾白僵菌的分离、培养及应用试验%Isolation,culture and application of Beauveria bassiana for the control of Dictyoploca japonica

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    杨世璋; 吴猛耐; 陈杰; 林琳; 付成松; 杜友兵; 蔡泽荣

    2001-01-01

    从银杏大蚕蛾僵死虫(蛹)尸上分离的白僵菌B21.1,经过室内扩大培养,生产出含孢量185亿孢子/g菌剂,以其2亿孢子/ml菌液对银杏大蚕蛾幼虫室内毒力测定,表现出很高的致病能力:4天死亡率近半,10天校正死亡率达到98.51%;林间喷雾防治,10天左右致病死亡近50%,21天校正死亡率为84.36%;与其它菌株(剂)试验比较,其致病死亡率和杀虫效果均明显高于其他各供试菌株,表现了较强的专化性。%Five isolates of Beauveria bassiana from different sources were examined for pathogenicity to Dictyoploca japonica. Compared with other four isolates,the isolates B21.1 derived from dead body of Dictyoploca japonica showed the best effectiveness.Two inoculation experiments,one in lab and the other in the field,were carried out.The mortality of pests caused by isolates B21.1 come to 98.51% in lab 10 days after inoculation and 84.36% in the field 21 days after inoculation.The results suggest that isolate of Beauveria bassiana from the host itself shows good prospect for the control of Dictyoploca japonica.

  11. Effects of Beauveria bassiana on virulence and cellular morphology of hemocytes of Ostrinia furnacalis%球孢白僵菌对亚洲玉米螟血细胞毒力和形态的影响

    Institute of Scientific and Technical Information of China (English)

    李福霞; 胡维娜; 胡琼波

    2016-01-01

    [Objective] To test the effect of Beauveria bassiana on SYSU-OfHe-C, a cell line from hemo-cytes of Ostrinia furnacalis. To provide a theory basis for application of B. bassiana in the prevention and treatment of O. furnacalis.[Method] MTT and CCK-8 were employed to test the virulence of B. bassiana on SYSU-OfHe-C. Optical microscope and scanning electron microscope were used to observe the growth of B. bassiana and its influence on the morphology of SYSU-OfHe-C cells. [Result]The IC50 values of B. bassiana against SYSU-OfHe-C cells at 24 hours after treatment were recorded as 2. 8 × 105 and 1. 4 × 105 mL-1 respectively by MTT and CCK-8. Furthermore, under real-time monitoring with optical micro-scope, encapsulization and phagocytosis were not found and B. bassiana was highly competitive when the conidia of B. bassiana were inoculated in the culture of SYSU-OfHe-C cells at logarithmic stage reaching a final concentration of 3. 5 × 104 mL-1 for B. bassiana. B. bassiana started to germinate at 10 hours after treatment, and meanwhile, SYSU-OfHe-C cells started to reduce in number and change in morphology. At 24 hours after treatment, B. bassiana grew the entire visual field. The nearly round SYSU-OfHe-C cells had elongated or turned into irregular shape. Aggregation, fragmentation, cavitation, protuberance and penetration of SYSU-OfHe-C cells caused by B. bassiana mycelia were observed.[Conclusion]B. bassiana can change cellular morphology of hemocytes of O. furnacalis, but cannot induce its immune responses.%【目的】测定球孢白僵菌Beauveria bassiana对亚洲玉米螟Ostrinia furnacalis血细胞系SYSU-OfHe-C的影响,为促进球孢白僵菌在防治亚洲玉米螟中的应用提供理论依据。【方法】采用MTT和CCK-8法测定球孢白僵菌对亚洲玉米螟血细胞系SYSU-OfHe-C的毒力,通过光学显微镜与扫描电镜观察球孢白僵菌生长及其对SYSU-OfHe-C细胞的影响。【结果】球孢白僵菌分

  12. Efeito de Beauveria bassiana (Bals. Vuill. e Metarhizium anisopliae (Metsch. Sorok. sobre características biológicas de Diatraea saccharalis F. (Lepidoptera: Crambidae - DOI: 10.4025/actascibiolsci.v30i2.3627 Effect of Beauveria bassiana (Bals. Vuill. and Metarhizium anisopliae (Metsch. Sorok. on Biological Characteristics of Diatraea saccharalis F. (Lepidoptera: Crambidae - DOI: 10.4025/actascibiolsci.v30i2.3627

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    Valéria Wanderley Teixeira

    2008-05-01

    Full Text Available A broca da cana-de-açúcar Diatraea saccharalis F. é considerada uma das principais pragas nas Américas. Entre os métodos de controle, o uso de fungos entomopatogênicos tem sido amplamente recomendado no manejo das pragas da cana-de-açúcar, incluindo outras lepidobrocas. Assim sendo, este estudo investigou os efeitos de diferentes concentrações de Beauveria bassiana (Bals. Vuill. e Metarhizium anisopliae (Metsch. Sorok. sobre parâmetros biológicos da broca da cana-de-açúcar. Larvas de terceiro instar de D. saccharalis foram tratadas com os fungos usando as concentrações de 103, 104 e 105 conídios mL-1. Larvas tratadas com 105 conídios mL-1 de B. bassiana tiveram menor sobrevivência (56,6%, comparadas com lagartas não-tratadas (90%. Adultos originados de larvas tratadas colocaram menor número de ovos, com menor viabilidade, e viveram menos, comparados com adultos originados de larvas não-tratadas. Larvas tratadas com M. anisopliae na concentração de 105 conídios mL-1 e adultos originados destas larvas também exibiram redução no desempenho, comparados aos insetos não-tratados. Os resultados indicam que B. bassiana e M. anisopliae, além de patogênicos às larvas de D. saccharalis, também interferem negativamente na sua biologia, mostrando potencial de uso contra esta praga.The sugarcane borer Diatraea saccharalis F. is considered one of the major sugarcane pests in the American continent. Among control methods, the use of entomopathogenic fungi has been broadly recommended to manage sugarcane pests, including other sugarcane borers. Therefore, this study investigated the effects of different concentrations of Beauveria bassiana (Bals. Vuill and Metarhizium anisopliae (Metch. Sorok on biological characteristics of the sugarcane borer. Third-instar larvae of D. saccharalis were fungi-treated using the concentrations of 103, 104 and 105 conidia mL-1. Larvae treated with 105 conidia mL-1 of B. bassiana showed lower

  13. Antagonism of Beauveria bassiana against Two Fusarium spp.Pathogenic to Cotton%球孢白僵菌对两种植物病原镰孢的拮抗作用

    Institute of Scientific and Technical Information of China (English)

    张胜利; 李增智

    2011-01-01

    体外测定了球孢白僵菌Beauveria bassiana对两种棉花病原镰孢——棉花枯萎病菌Fusarium oxysporium f.sp.vasinfecturn和棉花红腐病菌Fusarium verticillioides的拮抗作用。平板对峙培养时,两种镰孢的生长均受到明显抑制。不同含孢量的球孢白僵菌对两种镰孢的抑制效果不同,且抑制率与孢子浓度成显著的线性量效关系;在接种量为6×107孢子.mL-1时,对两种菌的抑制率均达到90%以上,对红腐病菌的抑制率稍高。白僵菌代谢液对两种植物病原菌也表现出抑菌作%In vitro antagonism of Beauveria bassiana,an entomopathogenic fungus,was tested against Fusarium oxysporium f.sp.vasinfecturn,pathogen of cotton wilt,and F.verticillioides,pathogen of cotton red rot.On dual culture,B.bassiana showed obvious inhibition on the growth of the two pathogens.Different doses of conidia had different inhibitory effects,with inhibition rate linearly correlated to inoculum,suggesting that the antagonism was associated with competition for nutrition and space.The inhibition on the two pathogens was both over 90% at inoculum of 6×107 spores·mL-1,slightly higher on F.verticillioide.The metabolite in SDY shake culture of B.bassiana also displayed inhibition,with inhibition rate linearly correlated to metabolite concentration,suggesting that the antagonism was also associated with antibiotic substances.Based on the comparison of inhibition rates,antibiosis was considered to contribute more than competition.In the analysis on the antibiotic metabolite,none of the lyophilized supernatant extracted by 4 different organic extractants showed obvious inhibition on the Fusarium spp.,while addition of crude extract of either polysaccharide or protein significantly inhibited mycelium growth of the two Fusarium spp.,suggesting that the main inhibitory substances are polysaccharide and protein with high molecular weight.

  14. Evaluacion del efecto de Beauveria bassiana en el control biológico de Varroa destructor, parasito de la abeja melífera (Apis mellifera en la finca Felisa en el municipio de los Patios, Norte de Santander - Evaluacion del efecto de Beauveria bassiana en el control biológico de Varroa destructor, parasito de la abeja melífera (Apis mellifera en la finca Felisa en el municipio de los Patios, Norte de Santander

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    Francy Liliana Duarte

    2013-08-01

    Full Text Available Beekeeping is an activity that produces significant benefits to agriculture and the environment; Through the pollination action of bees helps to increase productivity as well as biodiversity in the ecosystem. In recent years, has significantly increased prevalence of parasites in this species, particularly the Varroasis, a disease caused by the mite Varroa destructor. The causal agent produces losses between 30% and 50% of total production and in turn can cause damage to the quality of honey by the excessive use of chemicals for disease control and the slow and progressive deterioration health of producers. Noting this, we evaluated the incidence of fungus Beauveria bassiana in natural populations of Varroa destructor in acarofauna associated, and the remains of the hive by pathogenicity tests in laboratory conditions to evaluate the effect of the biocontrol entomopathogenic then enter in apiaries infected with the disease. According to the results obtained in the adult infestation of V. destructor in Apis mellifera Africanized sampling Felisa made on the farm in the municipality of Los Patios, recorded rates of infestation in hives from 3.4% to 8.3% on the infection status of breeding was 4.5% to 13.7%. In laboratory tests it was possible to observe thatthe fungus B. bassiana attacked by an effective control mites without harming bees, propolis and honey allowing the insect to continue their normal activities, controlling the disease in a biological, not chemical.

  15. 应用白僵菌和绿僵菌防治浙江双栉蝠蛾试验%Experiment of Beauveria bassiana and Metarhizium anisopliae on controlling Bipectilus zhejiangensis

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    童应华; 谢家冬; 祝德平

    2012-01-01

    Highly pathogenic Beauveria bassiana strain B3 and Metarhizium anisopliae strain Ma1291-2 were used for controlling Bipectilus zhejiangensis with different release methods. The results of 2-year controlling showed that effects of root irrigation was better than that of spreading with 2 strains, otherwise, the best effect was using 2 methods contemporarily (spreading + irrigation) in different time ( root irrigation in mid-March after spread in early March). There were no significant differences of the degraded shoot rate controlled between Beauveria bassiana and M. anisopliae in the first year, but the degraded shoot rate being controlled with strain Mal291-2 was lower significantly in the second year. By root spreading + irrigation with B3, the effects were 72.20% and 36.76% respectively in the first year and second year, and that of Mal291-2 was 79.28% and 48.70% respectively. Therefore, the 2 strains have application value for controlling Bipectilus zhejiangensis in forests, and the effect of application by root spreading + irrigation root was better in different time.%应用高致病力球孢白僵菌[ Beauveria bassiana( Bals.)Vuil] B3和金龟子绿僵菌[Metarhizium anisopliae( Metsch.)Sorokin] Ma1291-2菌株,以不同的施菌方式防治浙江双栉蝠蛾(Bipectilus zhejiangensis Wang),统计分析2a的防治效果.结果表明,2菌株均体现笋基灌施的防治效果较菌土撤施好,但以3月上旬进行撒施,3月中旬进行灌施(撒施+灌施)的防治效果最好.2菌株防治后第1年的退笋率无显著差异,而绿僵菌防治后第2年退笋率显著较低.以撒施+灌施防治后第1年和第2年,白僵菌B3菌株的防治效果分别为72.20%和36.76%,绿僵菌Ma1291-2菌株的防治效果分别为79.28%和48.70%.因此,2菌株均有林间应用价值,并以不同时间撒施+灌施的施菌方式防治,效果更佳.

  16. Virulence of Spores and Fermentation Filtrates of Beauveria bassiana Bb-2%球孢白僵菌菌株Bb-2孢子侵染力和代谢产物毒力

    Institute of Scientific and Technical Information of China (English)

    高萍; 胡强; 王彭

    2012-01-01

    The virulence of Beauveria bassiana strain Bb-2 to Plutella xylostella and Myzus persicae was assayed by its alive spores and fermentation filtrates. The results showed that Bb—2 was highly virulent at three consistencies, 1.1×107,1.1×108, 1.1× 109 ind./mL. At high consistency l.l×109 ind./mL, the cumulative mortality of P. Xylostella was 75.0%; and of M. Persicae was 92.5% in 4 d. At low consistency, the cumulative mortality was 36.7%; and of M. Persicae was 47.7%. The fermentation filtrates of strain Bb-2 were also with anti-pest active; and the virulence against M. Persicae was stronger than P. Xylostells.%利用球孢白僵菌菌株Bb-2(Beauveria bassiana Bb-2)活体孢子和代谢产物对2龄小菜蛾(Plutella xylostella)幼虫和桃蚜(Myzus persicae)进行毒力测定.结果表明,菌株Bb-2在1.1×107、1.1×108、1.1×109个孢子/mL 3个浓度下,对2龄小菜蛾幼虫和桃蚜均表现出较高的侵染力.在高浓度(1.1×109个孢子/mL)条件下,2龄小菜蛾幼虫的4d累计死亡率为75.0%,桃蚜的4d累计死亡率为92.5%.在低浓度(1.1×107个孢子/mL)条件下,2龄小菜蛾幼虫的4d累计死亡率为36.7%,桃蚜的4d累计死亡率为47.7%.菌株Bb-2发酵液对2龄小菜蛾幼虫和桃蚜均有一定的活性,且对桃蚜的活性明显高于对小菜蛾的活性.

  17. BIOLOGICAL CHARACTERISTICS OF THREE ISOLATES OF BEAUVERIA BASSIANA AND THEIR VIRULANCE AGAINST WHEAT APHIDS%球孢白僵菌的生物学特性及对小麦蚜虫的毒力

    Institute of Scientific and Technical Information of China (English)

    张永军; 王中康; 殷幼平; 裴炎

    2001-01-01

    Three isolates of Beauveria bassiana, Bb 02, Bb 03 and Bb 07, were cultured at different temperature and pH and on different media. The optimum temperature for their growth ranged from 25℃ to 30℃ and Bb 02 and Bb 07 grew well at pH 4.0 ~ 6.0. The addition of peptone and yeast extract stimulated their growth,while glucose and maltose had little promoting effect. All the three isolmes grew well on the media PPDA,SDAY and SMAY. Bioassay showed that they were strongly virulent against whest aphids,though their optimum dosage varied a little. The opimum concentration was 106/mL for Bb 02,107/mL for Bb 03 and 107 ~ 108/mL for Bb 07.%3株球孢白僵茵(Beauveria bassiana)Bb 02,Bb 03和Bb 07在不同温度,pH值和培养基上培养,结果表明,三者的最适生长温度在25℃~30℃间;Bb 02和Bb 07在pH4.0~6.0生长良好;适量的蛋白胨或酵母浸膏可促进菌株的生长,但对葡萄糖和麦芽糖的利用能力没有明显差异,三者在PPDA,SDAY和SMAY上生长良好。生物测定表明,3茵株对小麦蚜虫均具有较强的毒力,但各菌株的最适剂量略有差异,其中Bb 02以106mL-1较佳,Bb 03以107mL-1最好,Bb07则介于107mL-1~108mL-1之间。

  18. Pathogenicity of the entomopathogenic fungi Beauveria bassiana and Metarhizium anisopliae against larvae of Batocera davidis%白僵菌和绿僵菌不同菌株对橙斑白条天牛幼虫的致病力

    Institute of Scientific and Technical Information of China (English)

    童文钢

    2012-01-01

    橙斑白条天牛是福建省杨树的一种新害虫,应用不同球孢白僵菌(Beauveria bassiana)和金龟子绿僵菌(Metarhizium anisopliae)菌株对橙斑白条天牛幼虫进行致病力测定,旨在筛选出感染该虫的高致病力菌株,为生物防治提供新的资源.结果表明,接种后,该天牛幼虫的累积死亡率随时间的延长而逐渐增高,接种绿僵菌Ma1291-2和Ma1775菌株14 d后,幼虫的校正死亡率分别为91.69%和86.50%;接种白僵菌BbDp-06菌株14 d后,幼虫校正死亡率达71.05%.绿僵菌Mal291-2和Ma1775菌株对该幼虫的LT50分别为5.73和5.39 d,白僵菌BbDp-06菌株的LT50为8.02 d.Ma1291-2菌株对该幼虫的LC50为3.82×104孢子·mLL-1,白僵菌BbDp-06菌株的LC50为3.88 × 104孢子·mL-1.说明绿僵菌对该幼虫的致病力较强,尤其是Ma1291-2和Ma1775菌株,在橙斑白条天牛的生物防治中将有较好的应用价值.%Batocera davidis Deyrolle is a new pest of poplar in Fujian. In order to screen entomopathogenic fungi strains with high virulence against B. davidis larvae, and bioassay evaluation of ft bassiana and M. anisopliae against B. davidis larvae was carried out through dipping in spore suspension. The results showed that the cumulative corrected mortality of B. davidis larvae was gradually increased with time after inoculated with B. bassiana and M. anisopliae. The corrected mortality of B. davidis larvae on the 14th day after inoculated with Mal291-2 and Mal77S were 91.69% and 86.50% , respectively, and the mortality was 71.05% on the 14th day after inoculated with BbDp-06. The muscardine cadaver rate of larvae inoculated with M. anisopliae was significantly higher than that inoculated with ft bassiana. While LT50 value of the two M. anisopliae strains, Mal291-2 and Ma1775 were 5.73 and 5.39 days, respectively, and LT50 value of B. bassiana BbDp-06 was 8.02 days. LC50 value of two Btrains, Mal291-2 and BbDp-06 were 3.82 × 104 spore ·mL-1 and 3.88 × 104 spore·mL-1

  19. Changes of Detoxification Enzyme in the Larvae of Apriona germari Hope Infected by Beauveria bassiana%球孢白僵菌侵染对桑天牛幼虫解毒酶活性的影响

    Institute of Scientific and Technical Information of China (English)

    苏筱雨; 黄大庄; 李会平; 纪慧芳; 王晓红

    2007-01-01

    为明确球孢白僵菌(Beauveria bassiana)对桑天牛(Apriona germari Hope)的致病机制,探讨了球孢白僵菌侵染对桑天牛幼虫解毒酶系的影响,对用球孢白僵菌处理后不同时间桑天牛幼虫体内羧酸酯酶、谷胱甘肽-S-转移酶和乙酰胆碱酯酶活性进行了测定.结果表明球孢白僵菌对上述3种酶的活性有显著影响,其中对羧酸酯酶和谷胱甘肽-S-转移酶活性的影响,均表现出先刺激后抑制的作用;而对乙酰胆碱酯酶活性的影响则呈先抑制后激活再抑制再激活的趋势.

  20. STUDIES ON THE REGENERATION-REVERSION OF PROTOPLASTS AND KARYOTYPE OF BEAUVERIA BASSIANA%家蚕病原球孢白僵菌的原生质体再生回复及核型分析

    Institute of Scientific and Technical Information of China (English)

    时连根; 徐俊良

    2000-01-01

    家蚕病原球孢白僵菌(Beauveria bassiana)原生质体的分离制备、性状及再生回复,并用脉冲凝胶电泳(PFGE)技术分析了其核型.以6mg/mL Driselase为酶解液,0.7mol/L NaCl液(pH5.8)为渗透压稳定剂,在30℃下轻轻振荡处理幼嫩菌丝1.5h,是原生质体分离的适宜条件.原生质体的无核率为26.5%,有核率为73.5%,其中单核率为53.5%.再生回复的形式可观察到三种,其培养基的渗透压稳定剂以0.7mol/L葡萄糖较为适当.球孢白僵菌至少具有6条染色体,估算大小为2.5~6.6Mb,核型大小为26.5Mb.

  1. Genetic Engineering of Beauveria bassiana and environmental safety analysis of engineering strains%球孢白僵菌工程菌株构建及其环境安全性分析

    Institute of Scientific and Technical Information of China (English)

    吕丁丁; 李增智

    2013-01-01

    以球孢白僵菌(Beauveria bassiana)Bb202作为出发菌株,同时引入外源毒力基因,即北非蝎(Androctonus australis)昆虫特异性神经毒素多肽基因AaIT(GI:69545),构建高毒力的工程菌株Bb202T-7,并用SPSS软件统计分析,结果显示,Bb202T-7对松墨天牛(Monochamus alternatus)的杀虫效率提高了11.11%.研究目的是为生物防治提供性能良好的出发菌株,为高效杀虫剂的研制提供基础资源,克服真菌杀虫剂击倒昆虫所需时间长,对环境条件要求高等缺点,加速真菌杀虫剂的产业化步伐.同时对基因工程重组菌株的安全性问题进行分析,并提出解决方案.

  2. Studies on the Regeneration and Reversion of Protoplasts From Beauveria bassiana,a Pathogenic Fungus to Bombyx mori L.%家蚕病原白僵菌原生质体的再生回复研究

    Institute of Scientific and Technical Information of China (English)

    时连根; 柯林田; 费建明

    1999-01-01

    研究了家蚕病原白僵菌(Beauveria bassiana)原生质体的性状、再生回复形式与条件.家蚕病原白僵菌原生质体的无核率为26.5%,有核率为73.5%,其中单核率为53.5%.原生质体再生回复的形式可观察到3种,在位相差显微镜下不能判别原生质体有无再生回复能力.综合考虑原生质体再生频率和菌落生长发育速度,家蚕病原白僵菌原生质体再生回复用培养基的渗透压稳定剂以0.7mol/L葡萄糖较为适当.

  3. 继代培养对球孢白僵菌毒素产生水平的影响%Influence on the Toxin Production Level of Beauveria bassiana During Subculturing

    Institute of Scientific and Technical Information of China (English)

    张正坤; 孙召朋; 张语迟; 徐文静; 潘洪玉; 李启云

    2012-01-01

    继代培养对球孢白僵菌生物学特性、毒力及毒力相关因素等均会产生影响,研究了继代培养对球孢白僵菌毒素产生水平的影响.对野生球孢白僵菌D6-2和L1-1菌株进行室内培养基继代培养,利用高效液相色谱(HPLC)法对其代谢产物中已报道的2种毒素2-氯杀鼠灵酮和间甲基苯甲酸甲酯在不同世代的代谢水平进行了检测.对2株供试球孢白僵菌菌株,收集不同世代在SDAY培养基中培养相同时间后等量的白僵菌培养液,经过有机溶剂提取脂溶性部分,经HPLC定量检测,2个供试菌株各世代培养液中均只有F1代代谢产物中检测出了2-氯杀鼠灵酮,而F2—F5代未检测到,并且供试菌株各世代代谢产物中均未检测到间甲基苯甲酸甲酯;并且发现在继代培养过程中,有未知化合物产生水平未随着世代增加而发生显著变化.不同白僵菌菌株毒素的代谢种类存在差异,并且继代培养会降低某些毒素的代谢水平,也许是继代培养导致供试菌株毒力下降的原因之一;对于其他种类白僵菌毒素在继代培养过程中的代谢水平需进一步研究.%The biological character,toxicity and toxicity relative factors of Beauveria bassiana were influenced by subculturing,the effect on toxin production level of subculturing was investigated in this research. The B. bassiana strains D6-2 and LI -1 were subcultured on SDAY medium,to detect the influence of subculturing on production level at different generations of toxin methyl 3-methylbenzoate and 2-Coumaranone,by high performance liquid chromatography (HPLC) method. The inoculum at the same culturing time of different B. bassiana generations was collected,and its liposolubility portion was extracted by ethylalcohol and ethyl acetate sequentially,it showed via HPLC detection that 2-Coumaranone was detected in metabolic product of two strains F1 generation,but not in that of F2-F5 generations,and there was no methyl 3

  4. A Study of the Virulence of Beauveria bassiana Strains against Bipectilus zhejiangensis Wang Larvae%球孢白僵菌对浙江双栉蝠蛾幼虫的致病力研究

    Institute of Scientific and Technical Information of China (English)

    童应华

    2012-01-01

    8 strains of B. bassiana were used to test for pathogenicity against B. zhejiangensis larvae. The results indicated that the strain B3 had higher pathogenicity. After inoculating strain B3 with (1.0±0.5)× 106 conidia mL for 12 days, the correct mortality and the cadaver rate of larvae were (90.71±2. 16)% and (87.07±0.95)% respectively, and its median lethal time (LT50) was the shortest (6.20 days). The average conidia individual of cadaver was significantly higher with 5.75×1010 conidia per larva. The stronger lethal time of the strain B3 against larvae was the 6th day to 8th day. The median lethal concentration (LC50) of the 5th and 6th day to larvae were determined at 1.35×108 conidia perlarva and 1. 23×106 conidia perlarva respectively. It suggests that the strain B3 has great development application value, and strain B187 has certain application potential.%应用8株球孢白僵菌[Beauveria bassiana(Bals.) Vuill]菌株分别对浙江双栉蝠蛾幼虫进行致病力测定,结果表明:以浓度(1.0±0.5)×106孢子/mL的孢子悬液接种12d后,B3菌株对该虫有较强的致病力,其校正死亡率、僵虫率分别为(90.71±2.16)%和(87.07±0.95)%,致死中时(LT50)最短(6.20 d),且具较高的僵虫虫体含孢量(5.75×1010孢子/虫),该菌株对幼虫致死效应较强的时间在第6至第8d.接菌后在第5天和第6天,B3菌株对该虫的LC50分别为1.35×108孢子/mL和1.23×106孢子/mL.B3菌株有较大的开发应用价值,B187菌株也有一定的应用潜力.

  5. 球孢白僵菌与几种化学杀虫剂和除草剂的相容性%Compatibility of Beauveria bassiana to Some Chemical Insecticides and Herbicides

    Institute of Scientific and Technical Information of China (English)

    蔡悦; 张胜利; 李增智

    2011-01-01

    Fungal insecticides are often mixed with sublethal dose of chemical pesticides against pest insects in IPM to overcome their shortcomings of slow pathogenesis and low virulence.The compatibility of fungal spores to newly marketed pesticides should be investigated before the mixed use.In the present study,impacts of 7 insecticides and 3 herbicides on current market on conidial germination,growth speed and sporulation of 10 isolates of Beauveria bassiana were tested.Results showed that all the pesticides inhibited spore germination at conventional concentration and the inhibition declined as the concentration was decreased.Diluted by 10 times,8% cypermethrin,30% benazolin and 6.9% fenoxaprop-ethyl became not significantly inhibitory.All pesticides significantly inhibited mycelium growth under conventional concentration.Inhibition on some isolates by 1% emamectin benzoate,40% chlorpyrifos,4.5% beta-cypermethrin and 8% cypermethrin was significantly reduced after diluted by 10 times,while 8% cypermethrin even promoted growth of RCEF0894.All the pesticides inhibited sporulation extremely significantly.As an integrated comparison,when mixed with the above pesticides,RCEF0013 and RCEF0383 sporulated the most abundantly,while mycelia of RCEF0895,RCEF0896 and RCEF0897 grew the most rapidly.8% cypermethrin,30% benazolin and 6.9% fenoxaprop-ethyl showed a comparatively higher compatibility with B.bassiana,providing IPM program good candidates of insecticides and herbicides for mixed use with fungal insecticides.%针对真菌杀虫剂杀虫较慢、致死率较低的缺点,在IPM中常将其与亚致死量的化学农药混用,为此需不断测定其与各种新上市农药的相容性。本研究测定了当前农药市场上销售的7种杀虫剂和3种除草剂对10株球孢白僵菌Beauveria bassiana孢子萌发率、生长速度和产孢量的影响。结果表明,所有供试杀虫剂和除草剂在常规使用浓度下对球孢白僵菌分生孢子萌发都有显著

  6. 防治烟草甲白僵菌的仓储应用菌株选育%Screening and breeding ofBeauveria bassiana for controlling tobacco beetles

    Institute of Scientific and Technical Information of China (English)

    张晓敏; 杨辉; 施鸣; 胡大鸣; 刘爱英; 邹晓

    2015-01-01

    Screening and breeding of drought-resistant and high temperature-resistantBeauveria bassiana was conducted so as to make it adaptable in leaf tobacco warehouse. GZUIFR-GL5-08i stralns were screened out through a combined ultraviolet and microwave mutagenic experiment. Results showed that GZUIFR-GL5-08i sustalned stable growth on the condition of water potential 0.8Mpa and temperature 35℃. Content of protein, proiline, SOD, CAT, POD were higher than that before mutation, which showed similarity in draught-resistance mechanism between entomogenous fungi and plants.%为将白僵菌应用于烟叶储存醇化的仓储环境,对其进行抗干旱、耐高温菌株选育。通过温度筛选获得能够在35℃下生长的球孢白僵菌GZUIFR-GL5菌株。该菌株通过紫外线和微波在150s下的组合诱变和PEG胁迫筛选,得到具有生长稳定且能够耐受-0.8Mpa的较低水势和35℃环境条件的突变菌株GZUIFR-GL5-08i。测定GZUIFR-GL5-08i部分生理指标发现蛋白含量、脯氨酸、SOD、CAT、POD等含量均比诱变前菌株提高,反映了在抗旱机理方面虫生真菌与植物抗逆机理有相似性。

  7. 白僵菌经不同基质传代后对桑天牛幼虫的侵染力比较%Comparative Infectivity of Different Passage Reisolates of Beauveria bassiana to Apriona germari Larvae

    Institute of Scientific and Technical Information of China (English)

    李会平; 黄大庄; 杜邵华; 高洁

    2008-01-01

    以诱集自土壤中的对桑天牛(Apriona germari)幼虫具有较高致病性的球孢白僵菌(Beauveria bassiana)Bb00菌株为原发菌株R0,通过反复接种桑天牛幼虫分别获得菌株R1、R2、R3和R4,并反复在普通查氏培养基上传代分别获得菌株M1、M2、M3和M4.将R0、R2、R4和M2、M4菌株感染桑天牛幼虫进行生物测定,发现在普通培养基上传代,会导致菌株致病力的降低,而通过桑天牛幼虫传代培养可以提高菌株的致病力.R4、R2、R0、M2、M4菌株的LT50分别为3.23、4.01、4.13、4.78和6.34d,LC50如分别为0.686×106、1.470×106、3.050×106、7.940×106和9.580×106mL-1.表明通过菌株在桑天牛幼虫虫体上反复接种可以提高白僵菌对桑天牛幼虫的侵染力.

  8. 球孢白僵菌在红火蚁体表侵染的扫描电镜观察%Observation on infection process of Beauveria bassiana on cuticle of the red imported fire ant, Solenopsis invicta Buren (Hymenoptera: Formicidae), using scanning electron microscope

    Institute of Scientific and Technical Information of China (English)

    王龙江; 吕利华; 何余容; 谢梅琼

    2010-01-01

    利用扫描电镜观察了球孢自僵菌Beauveria bassiana Bb04菌株分牛孢子对红火蚁Beauveria bassiana 工蚁体壁的侵染过程.结果表明:分生孢子多分布在红火蚁工蚁节间膜、胸部的褶皱、气门、体壁的凹陷部位、刚毛窝附近.以及着生较密刚毛的足上.萌发的分生孢子在节间膜以及体表缝隙、刚毛窝及刚毛稀少的凹陷部位、胸部褶皱和足胫节处入侵.分生孢子在附着12 h后开始萌发,接种后18 h附着在节间膜处的孢子首先侵入成功,接种后24 h刚毛窝附近孢子萌发入侵,接种后60 h胸、腹和足等部位的孢子均成功穿透侵入表皮.分生孢子可以直接以芽管侵入表皮,也可以产生附着胞再侵入.

  9. Transcriptome analysis of Bemisia tabaci nymphs infected with Beauveria bassiana%感染球孢白僵菌的烟粉虱若虫免疫应答转录组分析

    Institute of Scientific and Technical Information of China (English)

    王登杰; 吴圣勇; 雷仲仁; 王海鸿

    2015-01-01

    Objectives] To screen the whitefly Bemisia tabaci (Gennadius) genes that respond to infection by Beauveria bassiana in order to further elaborate molecular mechanisms underlying the immune response to fungal infection. [Methods] High throughput sequencing technology was used in the transcriptome analysis of Bemisia tabaci 4th instar nymphs that were either infected, or uninfected (control), with B. bassiana. Differentially expressed genes (DEGs) and their functions, classifications and signaling pathways were analyzed using bioinformatic tools. [Results] 232 554 non-redundant Unigenes were identified, among which N50 and N90 were 1 153 bp and 260 bp in length, respectively. The average length of all Unigenes were 674 24 bp. Among the 1 166 DEGs (P<0.05) identified, 474 were up-regulated and 692 were down-regulated, and 405 are known to be involved in insect immune response. The identified Unigenes were enriched in 156 biological processes (66 402 Unigenes), 89 cellular components (27 645 Unigenes) and 154 molecular functions (73 417 Unigenes) by GO analysis. KEGG pathway analysis indicated that 1 145 DEGs were mapped to 309 distinctive pathways, and were enriched in 76 pathways. [Conclusion] Four hundred and five genes that are possibly involved in the immune recognition and defense of whitefly nymphs against B. bassiana infection were sequenced. The results provide a bioinformatic data base for using entomopathogenic fungi as a biological control for whitefly.%【目的】筛选烟粉虱 Bemisia tabaci (Gennadius)若虫应对白僵菌 Beauveria bassiana 侵染的应答基因,以进一步研究烟粉虱免疫反应的分子机制。【方法】采用新一代高通量测序技术对感染和非感染白僵菌的烟粉虱4龄若虫进行了测序分析,并筛选了差异表达基因;利用生物信息学工具对转录组测序得到的基因进行了功能注释、分类以及参与的信号通路展示。【结果】组装得到非冗余 Unigene 232554

  10. Integrated use of Beauveria bassiana and Bacillus thuringiensis serovar.tenebrionis for microbial biocontrol of colorado potato beetle%球孢白僵菌和苏云金杆菌拟步甲血清亚种联合使用防治马铃薯甲

    Institute of Scientific and Technical Information of China (English)

    斯蒂芬· 瑞特; Mark A Ramos

    2007-01-01

    This paper reviews the potential for using insect pathogens to control the Colorado potato beetle,Leptinotarsa decemlineata,and summarizes results from nearly 10 years of research by USDA-ARS-PPRU scientists aimed at developing methods and strategies for integrated use of Bacillus thuringiensis and Beauveria bassiana for biologically-based management of potato beetles in the northeastern USA.The described studies have resulted in 1) design of a tractor-mounted hydraulic spray system that improved targeting and consequent efficacy of microbial control agents, 2) discovery of synergism between the bacterial pathogen Bacillus thuringiensis (Bt) and the fungal pathogen Beauveria bassiana (Bb),3) findings that substantial mortality of beetle larvae treated with B.bassiana during foliar spray programs did not occur until after they entered the soil to pupate, and 4) observations that spray applications made in the evening (at sunset) were significantly, but not substantially, more effective than applications made during the morning hours.These findings have led to development of a bio-based pest management system based on three spray applications made in the following order at 3-5-day intervals:Bt alone,Bt and Bb mixed,and Bb alone.This spray program,with the applications initiated at 50% egg hatch,is intended to control the actively feeding larval stages (providing protection from defoliation) and reduce survivorship to the adult stage (providing long-term control by reducing populations of overwintering adults). In the initial test of the integrated management system,beetle control and potato yields were statistically equivalent to those in plots in which the beetles were controlled with chemical insecticides.Demonstration of the effectiveness of this program will continue over the next 2-3 field seasons.

  11. Preliminary study on nematicidal mechanism of secondary metabolites from Beauveria bassiana Snef23 to Meloidogyne incognita%球孢白僵菌次生代谢产物对南方根结线虫的作用机制初探

    Institute of Scientific and Technical Information of China (English)

    刘琼; 刘晓宇; 王媛媛; 朱晓峰; 段玉玺; 陈立杰

    2014-01-01

    为明确球孢白僵菌Beauveria bassiana对南方根结线虫Meloidogyne incognita的作用机理,从线虫的活动频率、呼吸强度、体液渗透压、总糖含量、可溶性蛋白含量以及乙酰胆碱酯酶( AChE )活性几方面测定了球孢白僵菌Snef23菌株次生代谢产物对南方根结线虫2龄幼虫( J2)的影响。结果表明:Snef23次生代谢产物可抑制南方根结线虫J2的活动频率和呼吸作用,降低其乙酰胆碱酯酶活性,增强虫体内容物的渗漏,干扰J2体内糖和可溶性蛋白的代谢,从而达到毒杀致死作用。研究结果为探明球孢白僵菌杀线虫的作用机制提供了理论依据,可为更好地利用球孢白僵菌及其他天然产物防控植物的线虫病害提供参考。%In order to understand the nematicidal mechanism of Beauveria bassiana against Meloidogyne incognita, the changes of physiological indexes of M. incognita second-stage juveniles (J2), including J2 locomotory behavior (mobile frequency), respiratory intensity, osmotic pressure of body fluid, total sugar content, soluble protein content and acetylcholinesterase activity etc, were detected after treatment with secondary metabolites of B. bassiana Snef23. The results showed that the secondary metabolites of B. bassiana Snef23 significantly reduced the mobile frequency and respiration intensity of M. incognita J2, inhibited the activity of acetylcholinesterase, increased the osmotic pressure of J2 body, interfered with the sugar and soluble protein metabolism of J2, which would lead to lethal effect on M. incognita. The results of present study provided theoretical data for elucidation of the nematicidal mechanism of B. bassiana Snef23, as well as for the prevention and control of plant nematode diseases by using B. bassiana and other nature productions in the future.

  12. 球孢白僵菌菌株交配型分子鉴定%Molecular Identification of Strain Mating Type of Beauveria Bassiana

    Institute of Scientific and Technical Information of China (English)

    王冰

    2014-01-01

    球孢白僵菌Beauveria bassiana是一种重要的虫生真菌,开发的真菌杀虫剂是一类优良的生物农药,其有性世代-球孢虫草Cordyceps bassiana也有潜在的经济价值.鉴定球孢白僵菌菌株的交配型,确立含有不同交配型标准菌株,进行菌株的快速配对,对于球孢虫草的人工栽培和球孢白僵菌的育种都具有重要的价值.通过多次重复试验,优化了扩增条件,保证了检测结果的稳定性和可靠性.该试验结果为检测球孢白僵菌菌株的交配型提供了一种简便、准确的方法,并为研究球孢白僵菌交配型基因的功能、人工栽培和育种奠定了基础.

  13. Cloning and Expression Analysis of DNA Methyltransferases Encoding Gene Dim-2 from Beauveria bassiana%球孢白僵菌DNA甲基转移酶基因Dim-2的克隆及其表达分析

    Institute of Scientific and Technical Information of China (English)

    汪超; 谢翎; 黄勃

    2014-01-01

    The full-length cDNA of Dim-2 gene was cloned from Beauveria bassiana using a RACE technique. The cDNA of Dim-2 had a lenght of 4021 bp, including an open reading frame (ORF) with 3429 bp encoding 1142 amino acids. The protein had a molecular mass of 128 kD with a calculated pI of 6.13. The Realtime-PCR and HPLC analysis indicated that the degree of DNA methylation varied along with fungal growth development. The results showed that both the expression level of Dim-2 gene and the degree of DNA methylation reached the minimum levels at initial stage of sporulation (the 7th day). It was inferred that there was an internal relationship between DNA methylation caused by Dim-2 gene and growth development in B. bassiana. The study will benefit future functional studies of DNA methylation in developmental control of entomopathogenic fungi.%本研究运用RACE技术,从球孢白僵菌中克隆出完整的DNA甲基转移酶基因Dim-2的编码区序列。该基因cDNA全长4021 bp,5′端非翻译区283 bp,3′端非翻译区303 bp,开放阅读框(ORF)3429 bp,编码1142个氨基酸。蛋白理论分子量为128 kD,理论等电点为6.13。结构域分析显示,该基因编码蛋白含有1个BAH结构域和合成5-甲基胞嘧啶的活性区域。Real-time PCR与HPLC检测表明,球孢白僵菌DNA甲基化程度会随着其生长发育的变化而不断改变。结果显示,球孢白僵菌Dim-2基因的转录表达量与基因组DNA的5-甲基胞嘧啶百分含量在菌体培养初始产孢阶段(即培养第7 d),均出现了1个低谷。这可能意味着球孢白僵菌Dim-2基因所引起的DNA甲基化与其生长发育之间具有内在的联系。本研究将为进一步探索DNA甲基化在虫生真菌生长发育中具体功能机制奠定基础。

  14. Changes in the Contents of Proteins and Amino Acids of Apriona germari Larvae Infected by Beauveria bassiana%桑天牛幼虫感染球孢白僵菌后蛋白质和氨基酸含量的变化

    Institute of Scientific and Technical Information of China (English)

    李会平; 黄大庄; 唐秀光

    2012-01-01

    To understand the relationship between the contents of proteins and free amino acids and the infection of Beauveria bassiana. Changes in the contents of proteins and free amino acids in haemolymph of Apriona germari larvae infected by B. bassiana were detected. The result showed that the content of proteins increased to the maximum in 3 days after inoculation,indicating the proteins and enzymes could be produced for the immunologic responses,and then declined. By using the polyacrylamide gelelectrophoresis( PAGE) technique, the change in protein composition in Apriona germari larvae infected by Beauveria bassiana was analyzed. The result showed that the band number increased after inoculation. There were significant differences in the band widths and staining intensities. The results of amino acids analysis showed that there were abundant necessary amino acids for the strain' s growth in A. germari larvae bodies, thus B. bassiana showed a high virulence to A. germari larvae. The content of free amino acids decreased in 24 h after inoculation, suggesting that the B. bassiana invaded into the insect body absorbed some amino acids from the blood as nutrition. The content of free amino acids had no further changes from 24 -72 h,and then increased sharply in 96 h and 120 h probably because of gradual loss of immunoregulation, destroy of tissue and disintegration of proteins. Before dead of the infected A. germari larvae,the contents of different amino acids changed differentially,suggesting that different growth phases of the isolate needed different amino acids.%桑天牛现称桑粒肩天牛(Apriona germari),是多种林木、果树、花卉的重要蛀干害虫,也是我国危害最严重、最难防治的害虫之一.近年来,其猖獗危害已成为我国养蚕业、林业及果树种植业等经济发展的严重障碍.球孢白僵菌(Beauveria bassiana)是一类广谱性昆虫病原真菌,能侵染15目149科的700多种昆虫(St Leger,2001;Blake et al.,2002

  15. 不同球孢白僵菌菌株生物学特性与其对家蚕致病力的关系%Relationship between Biological Characteristics of Beauveria bassiana ( Bals.) Vuill and Pathogenicity to Bombyx mori L.

    Institute of Scientific and Technical Information of China (English)

    骆海玉; 邓业成; 廖永梅; 李瑞钰

    2012-01-01

    [目的]探讨球孢白僵菌菌株的生物学特性与其对家蚕致病力的关系,为家蚕白僵病的防治提供科学依据.[方法]从我国各地收集6个白僵菌杀虫剂,并从广西养蚕区采集3个僵蚕样品,对从中分离纯化的菌株进行形态观察和分子鉴定,同时对家蚕进行致病力测定,比较各菌株菌落直径、产孢量、胞外蛋白酶产生水平等生物学特征指标的差异.[结果]获得的9个分离菌株均为球孢白僵菌(Beauveria bassiana Vuillemin);各菌株对家蚕都有较强致病力,且致病力有一定差异;各菌株菌落生长直径、产孢量及胞外蛋白酶活性均存在较大差异,并与其对家蚕的致病力之间存在相关关系.[结论]各菌株产孢量和胞外蛋白酶活性与其对家蚕的致病力之间存在显著正相关.%[Objective] This study was to investigate the relationship between biological characteristics of Beauveria bassiana ( Bals. ) Yuill and pathogenicity of Bombyx mori L. , with the aim to provide scientific basis for the control of white muscardine in Bombyx mori L. . [ Method ] The strains isolated and purified from the 6 Beauveria bassiana biocontrol agents from all over the country and the 3 white muscardine silkworms collected from Guangxi provincial silkworm rearing areas were identified by the morphological observation and molecular biology technology. The pathogenicity of silkworms to B. bassaina was determined, and the biological characteristics such as growth diameter, spore production amount and the extracellular protease activity of the different B. bassiana strains were compared. [ Result] The isolated 9 strains were al] B. bassaina (Bals. ) Vuillemin, and all strains had high pathogenicity to silkworm, but with different pathogenicities. The growth diameter, spore production amount and extracellular protease activity of different B. bassiana strains were also different, and showed correlation with the patheogenicity to silkworms

  16. 不同化性苜蓿切叶蜂(Megachile rotundata F.)成蜂对白僵菌敏感性的差异%Susceptibility of Different Voltine Adult Alfalfa Leaf-cutting Bee, Megachile rotundata F.(Hymenoptera: Megachilidae), to Beauveria bassiana Infection

    Institute of Scientific and Technical Information of China (English)

    李茂海; 李建平; 杨桂华; 刘金文; 侯云龙; 曲文利

    2007-01-01

    室内测定了不同化性苜蓿切叶蜂成蜂对球孢白僵菌(Beauveria bassiana (Balsamo))的敏感性.在28℃,RH60%的条件下,喷施白僵菌后二化种群死亡速度较快,72h后死亡率为67%,而一化种群72h后死亡率为38%.结果表明,成蜂对白僵菌的敏感性在化性间存在差异,二化种群成蜂对球孢白僵菌具有较高的敏感性(P<0.05),抗性较差.

  17. Identification of a Beauveria bassiana Strain with High Pathogenicity to Bombyx mori and Its Application in Bombyx Batryticatus Production%一株感染家蚕的高致病性白僵菌的鉴定及在僵蚕生产中的应用

    Institute of Scientific and Technical Information of China (English)

    邢东旭; 李丽; 廖森泰; 肖阳; 李庆荣; 叶明强; 杨琼

    2014-01-01

    僵蚕是传统中药材,筛选对家蚕具有高致病性的球孢白僵菌用于提高规模化生产僵蚕的产量和品质.从蚕区采集的僵蚕虫体分离纯化到一株高致病力的白僵菌,通过形态学鉴定和rRNA ITS序列系统发育分析,确定该菌株为球孢白僵菌(Beauveria bassiana),命名为Beauveria bassiana JC-1.优化该菌株应用于僵蚕生产中的接种菌液浓度为1×106~5×106 mL-1,用此浓度的菌液喷洒接种5龄起蚕后5~6d,幼虫大量死亡,僵化率达90%以上,万头蚕可生产僵蚕约5 kg.利用该菌株感染家蚕收获所得僵蚕的各项质量指标均符合《中国药典》中的有关标准,且僵蚕的总灰分和酸不溶性灰分含量较市售僵蚕更低.初步认为该菌株在僵蚕的标准化、规模化生产中具有应用价值.

  18. Optimization of submerged culture conditions for blastospore and biomas s production of Beauveria bassiana%球孢白僵菌液体生长营养和培养条件优化

    Institute of Scientific and Technical Information of China (English)

    张亚平; 吴圣勇; 王海鸿; 雷仲仁

    2014-01-01

    The principal objectives of this study were to determine the optimal liquid culture conditions in shake flasks for maximal biomass and blast spores of Beauveria bassiana. The results showed that glucose was the suitable carbon source,and yeast powder was the suitable nitrogen source for the growth of strain DZDC-9. Adding 0.000 5 mol/L KCl into the submerged culture can significantly enhance the yield of biomass and blast spores. The biomass reached(13.184±0.328 6)g/L after 60 h. The best culture conditions were shake flasks with one-fifth nutrient so-lution and initial conidial concentration of 107 spores/mL in a rotary shaking incubator at 200 r/min at 26 ℃ , which could increase the biomass and shorten the incubation time. Using the selected nutrition and culture condi-tions to produce conidia,the yield could achieve 22.30±1.78 per kilogram of rice.%以菌株液体发酵过程中菌丝干重和芽生孢子浓度为测试指标,对球孢白僵菌DZDC-9菌株液体发酵营养需求进行优化,结果表明,菌株生长的适宜碳源为葡萄糖,适宜氮源为酵母粉,在基础培养液中添加0.0005 mol/L KCl可以显著提高菌丝和芽生孢子浓度。通过单因素分析和正交设计对不同培养条件下菌丝生长量进行测定,结果表明,在培养温度26℃,培养液装样量1/5,初始接菌量107个/mL,转速200 r/min的培养条件下,菌株液体发酵能在60 h达到最大菌丝生长量(13.184±0.3286)g/L。利用筛选出的培养基和培养条件进行液固双相发酵试验,得到的孢子粉产量为(22.30±1.78)g/kg大米,显著高于优化之前的产量(13.04±1.90)g/kg大米。

  19. Virulence of Beauveria bassiana CQBb111 Strain to Diaphorina citri and Neoseiulus cucumeirs%白僵菌CQBb111菌株对柑橘木虱和胡瓜新小绥螨的毒力差异

    Institute of Scientific and Technical Information of China (English)

    张艳璇; 孙莉; 林坚贞; 陈霞; 季洁

    2013-01-01

    Virulence of Beauveria bassiana CQBb111 strain at different concentration to the adults of Diaphorina citri and the female adults of Neoseiulus cucumeirs was evaluated in the laboratory. The corrected accumulative mortality caused by suspension liquid of 1.0×104 conidia·mL−1 against D. citri and N. cucumeir were 92.68%and 4.05%, respectively, 10 d after treatment. LC50 against D. citri and N. cucumeir were 2.24×109 and 5.4×1012 conidia·mL−1, respectively,at the 3rd d after treatment. At the same treatment time, concentration that caused 50%death of D. citri was only slightly virulent to N. cucumeir. LT50 against D. citri was 2.88 d at the concentration of 1.0×108 conidia·mL−1, but the corrected accumulative mortality of N. cucumeir was only 10.7% at 2.88 d. LT50 against D. citri was much smaller than thatagainst N. cucumeir at the same concentration of CQBb111 strain. The strain can achieve good control of adults of D. citri while exert slightly negative influence on female adults of N. cucumeir and it can be used in combination with N. cucumeir to control D. citri and huanglongbing vectored by D. citri.%  本文用球孢白僵菌 CQBb111菌株的分生孢子配成不同浓度的悬浮液对柑橘木虱成虫和胡瓜新小绥螨雌成螨的毒性进行了测定。1.0×104孢子·mL−1的悬浮液作用第10 d的柑橘木虱和胡瓜新小绥螨累计校正死亡率分别为92.68%、4.05%,同浓度的孢子悬浮液对柑橘木虱的致死率远高于其对胡瓜新小绥螨的致死率。柑橘木虱和胡瓜新小绥螨第3 d的LC50为2.24×109、5.4×1012孢子·mL−1,同一时间下,对柑橘木虱致死率达50%的悬浮液对胡瓜新小绥螨的影响甚微。浓度为1.0×108孢子·mL−1时,柑橘木虱的LT50为2.88 d,而胡瓜新小绥螨此时校正死亡率仅为10.7%,同浓度的孢子悬浮液对柑橘木虱的LT50远小于胡瓜新小绥螨的LT50。该菌株对柑橘木虱成虫有很好的防治效果而对胡瓜

  20. 不同生长条件下球孢白僵菌菌株的生长状况及对美国白蛾幼虫的致病力研究%Effect of Different Condition on Mycelium Growth of a Beauveria bassiana Strain and Its Pathogenicity against Hyphantria Cunea Larvae

    Institute of Scientific and Technical Information of China (English)

    王伦

    2011-01-01

    试验所用球孢白僵菌(Beauveria bassiana)是从受侵染的美国白蛾[Hyphantria Cunea(Drury)]蛹体内分离出的菌株,再进行纯化,获得球孢白僵菌纯菌落。研究测定了不同生长条件对球孢白僵菌菌株的营养生长、孢子萌发以及对不同龄期美国白蛾幼虫致病力的影响。结果表明:相对湿度为90%时,菌株的营养生长最好,随着相对湿度的增大,孢子萌发速度、萌发率也增高;相对湿度为100%时孢子萌发率达到最高。经紫外线照射后菌株的营养生长速度缓慢,孢子萌发率降低。相对湿度为90%时被该菌株感染的美国白蛾幼虫的死亡率最高,3龄幼虫的死亡率比6龄幼虫高。%In this experiment,the mycelium of Beauveria bassiana was separated from infected hyphantria cunea pupae before it was purified.The effect of humidity and ultraviolet radiation on the mycelium growth,spores germ ination and the pathogenicity against hyphantria cunea larvae of a Beauveria bassiana strain were tested.The results showed that the optmium relative humidity for mycelium growth and spores germ ination was RH 90%,and at RH 90% hyphantria cunea larvae had highest motality.The germ ination speed and rates of spores increased as the RH increasing,and the 3rd larvae died more quickly than the 6th under 90% RH.The rates of spores decreased for irradiated by ultraviolet radiation.

  1. 不同温、湿度下球孢白僵菌Bb00菌株的生长状况及对桑天牛的致病力研究%Effect of Temperature and Humidity on Mycelium Growth of A Beauveria bassiana Strain and its Pathogenicity Against Apriona germari Larvae

    Institute of Scientific and Technical Information of China (English)

    王晓红; 黄大庄; 李会平; 纪慧芳; 杜绍华

    2007-01-01

    球孢白僵菌(Beauveria bassiana)可作为多种农业害虫的真菌杀虫剂.研究测定了不同温、湿度条件对球孢白僵菌Bb00菌株的营养生长、孢子萌发率以及对桑天牛(Apriona germari)幼虫致病力的影响.结果表明: 25 ℃时该菌株营养生长最好,孢子萌发速度快且萌发率高,在此温度下菌株对桑天牛幼虫的致病力也最强;相对湿度为95%~100%时菌株的营养生长最好,随着相对湿度的增大孢子萌发速度、萌发率也增高,相对湿度100%时达到最高,此时被该菌株感染的桑天牛幼虫的死亡速度快,死亡率也最高.

  2. Assessment of Efficacy of Beauveria bassiana (Bals.) Vuill WP against Dendrolimus punctatus%球孢白僵菌WP防治马尾松毛虫药效评价

    Institute of Scientific and Technical Information of China (English)

    胡凌云; 汤均友

    2012-01-01

    In order to find a biological control method against Dendrolimus punctatus,the authors conducted a test of using 4×1010spores which existed in one gram Beauveria bassiana(Bals.)Vuill and WP to control Dendrolimus punctatus.Four concentrations and four different treatment time were used.The results showed that Using 4×1010 spores which existed in one gram Beauveria bassiana(Bals.)Vuill and WP at the dose of 3 000 gram per hectare could control Dendrolimus punctatus efficiently;the efficacy reached 90.33% on the 7th day after treatment;the efficacy reached 96.00% on the 15th day after treatment;Usage of 4×1010spores which existed in one gram Beauveria bassiana(Bals.)Vuill and WP could kill the insects quickly and efficiently.Therefore,it could be used as an effective measure to control Dendrolimus punctatus.%为寻找马尾松松毛虫的生物防治1种有效方法,笔者对400亿孢子/g球孢白僵菌WP进行了4种浓度、4种用药后时间的方法进行对比差异实验。结果表明:400亿孢子/g球孢白僵菌WP3 000 g/hm2对马尾松毛虫具有较好的防治效果,药后7 d防效达90.33%,药后15 d防效达96.00%。400亿孢子/g球孢白僵菌WP防治马尾松毛虫杀虫速度快、效果好,是马尾松毛虫1种有效的生物防治措施。

  3. A report on the experiment to take out technology of Beauveria bassiana spore pink by machanical isolation%白僵菌孢子粉机械分离提取技术试验报告

    Institute of Scientific and Technical Information of China (English)

    李加勒

    1984-01-01

    白僵菌Beauveria bassiana孢子粉,是普通白僵菌原粉经过加工处理得到的含孢量很高的白僵菌制剂.它具有颗粒细、体积小、药效高、便于贮运、适用于超低容量喷雾等特点.本文提出了白僵菌孢子粉的机械分离提取技术,内容包括工作原理、机械构造、性能、工艺流程、操作技术及经济效益等.

  4. 不同球孢白僵菌菌株对马尾松毛虫致病性评价%Pathogenicity Evaluation of the Entomopathogenic Fungi Beauveria bassiana against Dendrolimus punctatus

    Institute of Scientific and Technical Information of China (English)

    汤强; 刘玉军; 萧晟; 方玲

    2013-01-01

    选择9个球孢白僵菌Beauveria bassiana菌株对4龄马尾松毛虫Dendrolimus punctatus幼虫进行毒力测定.结果显示,菌株Bb1900对马尾松毛虫幼虫的校正死亡率达96.16%,侵染率达86.67%,在孢子浓度为1×107孢子/mL时的半致死中时仅为4.62 d,显示出对目标害虫的较强毒力,在马尾松毛虫的生物防治中将有重要的应用价值.

  5. Rosalia longicorn Rosalia alpina (LINNAEUS, 1758 (Coleoptera: Cerambycidae as a host of the entomopathogenic fungus Cordyceps bassiana LI, LI, HUANG

    Directory of Open Access Journals (Sweden)

    Bartnik Czesław

    2016-06-01

    Full Text Available The paper describes, for the first time, the occurrence of the entomopathogenic fungus Cordyceps bassiana (anamorph: Beauveria bassiana on the imago of the endangered beetle Rosalia longicorn Rosalia alpina from the Low Beskid Mountains (the Carpathians, SE Poland. Furthermore, an isolate of the saprotrophic fungus Hypoxylon fragiforme was obtained as a result of laboratory tests on R. alpina specimens. Relationships between the identified fungi and R. alpina are discussed.

  6. USO DE BIOINSECTICIDAS PARA EL CONTROL DE PLAGAS DE HORTALIZAS EN COMUNIDADES RURALES

    OpenAIRE

    Cipriano García-Gutiérrez; María Berenice González-Maldonado

    2010-01-01

    Se evaluó el uso de bioinsecticidas elaborados a base de hongos entomopatógenos (Beauveria bassiana, Metarhizium anisopliae y Paecilomyces fumosoroseus); la producción se hizo de manera artesanal en comunidades rurales y se utilizaron para el control de plagas de hortalizas. Los productos fueron formulados con celite® y se usaron a dosis de 240g (1.2x1012 esporas/ha), para el control de plagas sobre plantaciones de lechuga Lactuca sativa L., rábano Rhapanus sativus, cebolla Allium cepa L., re...

  7. 球孢白僵菌选择性培养基的筛选和检测应用%Selection of culture medium of Beauveria bassiana parasitized in Strobilomyla and inspection of pathogen in soil

    Institute of Scientific and Technical Information of China (English)

    史红霞; 胡明龙; 阎峻

    2002-01-01

    报道了寄生球果花蝇Strobilomyia spp.的球孢白僵菌Beauveria bassiana选择性培养基的筛选以及应用该培养基检测土壤中球孢白僵菌动态的研究结果.为有效地检测施用球孢白僵菌防治球果花蝇的土壤中球孢白僵菌的变化,以Doberski的选择性培养基为基础,筛选出对球孢白僵菌具有较强选择性的选择性培养基,可回收土壤中球孢白僵菌孢子达79.5%.用该培养基检测球孢白僵菌在施菌土壤中第2年的存活率为0.9%~2.8%,它在土壤中有一定的持效性.表4参6

  8. The pathogenicity of Beauveria bassiana to Helicoverpa armigera under different temperatures and humidities%不同温、湿度下白僵菌对棉铃虫幼虫的致病力

    Institute of Scientific and Technical Information of China (English)

    孙鲁娟; 吴孔明; 郭予元

    2001-01-01

    测试了不同温、湿度下白僵菌Beauveria bassiana对棉铃虫Helicoverpa armigera幼虫的致病力.实验设置了10、15、20、25、30℃5个温度水平,用高浓度(1×108孢子/mL)、中浓度(1×107孢子/mL)、低浓度(1×106孢子/mL)的白僵菌孢子液分别感染1~4龄的棉铃虫.结果表明:三个浓度处理的棉铃虫在温度为25℃时的致死中时(LT50)最短,死亡速度最快,死亡率最高;高于或低于此温度时,棉铃虫的LT50延长,死亡速度减慢.相对湿度发生变化时,感病棉铃虫死亡速度和死亡率明显不同.相对湿度为95%左右时,棉铃虫死亡速度最快,死亡率最高;相对湿度低于70%时,棉铃虫死亡率显著降低.

  9. 球孢白僵菌对稻水象甲成虫的毒力测定%Bioassay of Beauveria bassiana against the rice water weevil,Lissorhoptrus oryzophilus Kuschel

    Institute of Scientific and Technical Information of China (English)

    蒋明星; 商晗武; 程家安

    2002-01-01

    @@ 稻水象甲Lissorhoptrus oryzophilus Kuschel是我国检疫性水稻害虫,主要以幼虫食根致害.化学防治是该虫田间治理的主要途径,但由于其施药时间较早(一般在移栽后2周内),不利于稻田早期天敌群落的建立,故有必要探讨其它治理途径.在生物防治方面,国内外已从该虫成虫上分离到多种病原生物,如球孢白僵菌Beauveria bassiana、绿僵菌Metarhizium anisopliae、琼斯多毛菌Hirsutella jonesii等,并对前两者的致病力及田间防治进行了研究,表明微生物杀虫剂在该虫的治理中具有一定的应用潜力.笔者从该虫上分离到6个球孢白僵菌菌株,并采用时间-剂量-死亡率模型的方法对其毒力进行了评价.

  10. 不同交配型球孢白僵菌在玉米植株中的定殖及其交配型基因变异比较研究%A Comparision Studies of Different Mating-type Beauveria bassiana Colonization in Corn, Zea mays, and Mating-type Gene Mutation

    Institute of Scientific and Technical Information of China (English)

    李晓慧; 汪洋洲; 张军; 徐文静; 张正坤; 刘艳微; 孟鑫睿; 冯树丹; 李启云

    2014-01-01

    本文通过分析两种不同交配型球孢白僵菌菌株(MAT1−1−1,I型;MAT1−2−1,II型)在玉米植株中的定殖效率和回收菌株交配型基因序列的变异,拟揭示球孢白僵菌在玉米植株内生定殖过程中玉米对不同交配型菌株的选择规律、不同交配型菌株对环境的适应性能力。试验分为4个接种组,接种菌株为I型交配型菌株(BbOFDH1−5)(处理B5)、II型交配型菌株(BbDPSD2)(处理B2)、两个菌株等量混合(处理B5+B2)、对照组(处理CK),处理组B5、B2、B5+B2接种孢子浓度均为108孢子/mL,对照组接种量为零。结果表明,处理B5、B2、B5+B2三组中均有球孢白僵菌在玉米叶片中定殖,其中,交配型II菌株处理组B2中内生定殖效率高,检测率为33.00%;混合处理组B5+B2中交配型II菌株比I型菌株回收比率高,回收比为12:10。交配型基因序列分析显示,交配型I菌株处理组B5和混合处理组B5+B2的回收菌株中I型交配型基因非编码区序列出现多个位点突变,编码区仅在3个位点出现突变;交配型II菌株处理组B2和混合处理组B5+B2中II型交配型基因序列均没有发生变异。以上结论说明球孢白僵菌在玉米植株定殖过程中,交配型II菌株BbDPSD2定殖率较I型菌株BbOFDH1−5高;玉米植株更倾向于选择II型交配型菌株作为内生菌株;交配型基因MAT1−1−1比MAT1−2−1更易发生突变表明I型菌株对自然环境的适应性更强。%In this study, colonization efficiency of the two mating-type Beauveria bassiana (MAT1−1−1, mating-type I; MAT1−2−1, mating-type II) in maize was assessed and the mating-type gene sequences of recovered B. bassiana strains were analyzed to reveal the host plants selection rules between the two mating-type strains and the environmental adaptability of different mating-type strains during colonization process. Four inoculation

  11. Effects of multiple applications of a Beauveria based biopesticide on Varroa destructor (Acari: Varroidae) densities in honey bee (Hymenoptera: Apidae) colonies

    Science.gov (United States)

    A biopesticide, formulated with a strain of Beauveria bassiana isolated from varroa mites, was tested in an experiment in southern France and the results were were compared to published results from previous experiments with the same biopesticide. Bee colonies were treated either with biopesticide, ...

  12. A Beauveria phylogeny inferred from nuclear ITS and EF1-alpha sequences: evidence for cryptic diversification and links to Cordyceps teleomorphs.

    Science.gov (United States)

    Rehner, Stephen A; Buckley, Ellen

    2005-01-01

    Beauveria is a globally distributed genus of soil-borne entomopathogenic hyphomycetes of interest as a model system for the study of entomopathogenesis and the biological control of pest insects. Species recognition in Beauveria is difficult due to a lack of taxonomically informative morphology. This has impeded assessment of species diversity in this genus and investigation of their natural history. A gene-genealogical approach was used to investigate molecular phylogenetic diversity of Beauveria and several presumptively related Cordyceps species. Analyses were based on nuclear ribosomal internal transcribed spacer (ITS) and elongation factor 1-alpha (EF1-alpha) sequences for 86 exemplar isolates from diverse geographic origins, habitats and insect hosts. Phylogenetic trees were inferred using maximum parsimony and Bayesian likelihood methods. Six well supported clades within Beauveria, provisionally designated A-F, were resolved in the EF1-alpha and combined gene phylogenies. Beauveria bassiana, a ubiquitous species that is characterized morphologically by globose to subglobose conidia, was determined to be non-monophyletic and consists of two unrelated lineages, clades A and C. Clade A is globally distributed and includes the Asian teleomorph Cordyceps staphylinidaecola and its probable synonym C. bassiana. All isolates contained in Clade C are anamorphic and originate from Europe and North America. Clade B includes isolates of B. brongniartii, a Eurasian species complex characterized by ellipsoidal conidia. Clade D includes B. caledonica and B. vermiconia, which produce cylindrical and comma-shaped conidia, respectively. Clade E, from Asia, includes Beauveria anamorphs and a Cordyceps teleomorph that both produce ellipsoidal conidia. Clade F, the basal branch in the Beauveria phylogeny includes the South American species B. amorpha, which produces cylindrical conidia. Lineage diversity detected within clades A, B and C suggests that prevailing morphological

  13. Genes involved in virulence of the entomopathogenic fungus Beauveria bassiana

    NARCIS (Netherlands)

    Valero-Jiménez, Claudio A.; Wiegers, Harm; Zwaan, Bas J.; Koenraadt, Constantianus J.M.; Kan, van Jan A.L.

    2016-01-01

    Pest insects cause severe damage to global crop production and pose a threat to human health by transmitting diseases. Traditionally, chemical pesticides (insecticides) have been used to control such pests and have proven to be effective only for a limited amount of time because of the rapid spre

  14. Desarrollo de un prototipo de formulación con hongos entomopatógenos para el manejo de Demotispa neivai Bondar (Coleoptera: Chrysomelidae) / Development of a prototype of making with entomopathogenic fungi for management of Demotispa neivai Bondar (Coleptera: Chrysomelidae)

    OpenAIRE

    Martínez Castrillón, Luis Carlos

    2010-01-01

    Entre los factores que limita la producción en el cultivo de la palma de aceite se encuentra el insecto Demotispa neivai Bondar, el cual ocasiona daños hasta de un 100% en su superficie. Durante el desarrollo de un prototipo de formulación con hongos entomopatógenos para el manejo de D. neivai fue necesario evaluar la patogenicidad de tres aislamientos de hongos entomopatógenos en adultos del insecto, utilizando como inóculo dos cepas de Beauveria bassiana (B018, B025), y una de Metarhizium a...

  15. Selección de Aislamientos de Hongos Entomopatógenos para el Control de Huevos de la Polilla del Tomate, Tuta absoluta Meyrick (Lepidoptera: gelechiidae) Entomopathogenic Fungi Isolates Selection for Egg Control of Tomato Moth Tuta absoluta Meyrick (Lepidoptera: gelechiidae) Eggs

    OpenAIRE

    Marta Rodríguez S.; Marcos Gerding P.; Andrés France

    2006-01-01

    Se realizó un estudio en laboratorio sobre la patogenicidad de 64 aislamientos de Metarhizium anisopliae var. anisopliae y 70 de Beauveria bassiana, en huevos de polilla del tomate Tuta absoluta. La primera evaluación se realizó por aplicación directa de suspensiones de 10(7) conidias mL-1 para cada aislamiento, con el sistema de pulverización de la torre de Potter. La mortalidad y esporulación sobre huevos fueron significativamente mayores con los aislamientos M. anisopliae Qu-M558 y B. bass...

  16. Selection of Entomopathogenic Fungi to Control Varroa destructor (Acari: Varroidae Selección de Hongos Entomopatógenos para el Control de Varroa destructor (Acari: Varroidae

    Directory of Open Access Journals (Sweden)

    Marta Rodríguez

    2009-12-01

    Full Text Available The aim of this work was select entomopathogenic fungi tolerant to temperatures inside the brood area of honey bees (Apis mellifera for to control Varroa destructor. For this purpose, 50 Beauveria bassiana (Balsamo Vuillemin and 48 Metarhizium anisopliae (Metschn. Sorokin isolates were evaluated at 30 and 35 ºC. For each isolate, colony discs of 5 mm with mycelium were placed in the center of a Petri dish with Sabouraud dextrose agar (SDA medium. The dishes were incubated at 30 and 35 °C, without light. Radial growth of each colony was measured daily. All the B. bassiana and M. anisopliae isolates presented a lineal growth rate at a temperature of 30 ºC. However, at 35 ºC, most of the isolates did not grow, except three B. bassiana and 14 M. anisopliae isolates (P El objetivo de este trabajo fue seleccionar hongos entomopatógenos tolerantes a las temperaturas del nido de cría de las abejas (Apis mellifera, para ser utilizados en el control de Varroa destructor. Se evaluaron 50 aislamientos de Beauveria bassiana (Balsamo Vuillemin y 48 de Metarhizium anisopliae (Metschn. Sorokin a temperaturas de 30 y 35 ºC. Discos de agar de 5 mm de diámetro con micelio de colonias de cada aislamiento, se depositaron en el centro de placas Petri con medio agar Sabouraud dextrosa (ASD. Las placas fueron incubadas a 30 y 35 °C y oscuridad y diariamente se midió el radio de cada colonia. Todos los aislamientos de B. bassiana y M. anisopliae var. anisopliae presentaron una tendencia lineal a través del tiempo a temperaturas de incubación de 30 °C. A 35 °C la mayoría de los aislamientos no crecieron, excepto tres aislamientos de B. bassiana y 14 de M. anisopliae (p < 0,001. Estos aislamientos fueron seleccionados para realizar pruebas de patogenicidad sobre V. destructor, aplicando una suspensión de 10(7 conidias mL-1. El aislamiento más efectivo fue Qu-M845 de M. anisopliae (p = 0,0033, produjo una mortalidad de 85%. La capacidad patogénica de este

  17. Comparative evaluation of five Beauveria isolates for housefly (Musca domestica L.) control and growth optimization of selected strain.

    Science.gov (United States)

    Mishra, Sapna; Malik, Anushree

    2012-11-01

    Pathogenic potential of five native Beauveria isolates was assessed against housefly adult and larvae in laboratory bioassays. Beauveria isolate Beauveria bassiana HQ917687 showed highest virulence with 72.3 and 100 % mortality of larvae and adults of Musca domestica, respectively. Other Beauveria isolates caused 36-52 % housefly larval mortality while the adult mortalities varied between 72 and 82 %. B. bassiana HQ917687 also showed the fastest killing activity with LT(50) of 4 days (for larvae) and 3 days (for adults). This isolate showing highest virulence was selected for its growth optimization in terms of biomass and spore production using response surface methodology. The optimum value of temperature, yeast extract, and pH for maximum biomass and spore production was predicted as 27 °C, 5.00 g/l, and 6.75, respectively. Temperature was found to be the most critical factor influencing biomass and spore yield of the fungus and even nullified the effects of other factors at sufficiently higher value. The results obtained in this study depict the significance of appropriate strain selection and process parameter optimization in order to facilitate mass production of biocontrol agents.

  18. Development of a method for detection and quantification of B. brongniartii and B. bassiana in soil.

    Science.gov (United States)

    Canfora, L; Malusà, E; Tkaczuk, C; Tartanus, M; Łabanowska, B H; Pinzari, F

    2016-01-01

    A culture independent method based on qPCR was developed for the detection and quantification of two fungal inoculants in soil. The aim was to adapt a genotyping approach based on SSR (Simple Sequence Repeat) marker to a discriminating tracing of two different species of bioinoculants in soil, after their in-field release. Two entomopathogenic fungi, Beauveria bassiana and B. brongniartii, were traced and quantified in soil samples obtained from field trials. These two fungal species were used as biological agents in Poland to control Melolontha melolontha (European cockchafer), whose larvae live in soil menacing horticultural crops. Specificity of SSR markers was verified using controls consisting of: i) soil samples containing fungal spores of B. bassiana and B. brongniartii in known dilutions; ii) the DNA of the fungal microorganisms; iii) soil samples singly inoculated with each fungus species. An initial evaluation of the protocol was performed with analyses of soil DNA and mycelial DNA. Further, the simultaneous detection and quantification of B. bassiana and B. brongniartii in soil was achieved in field samples after application of the bio-inoculants. The protocol can be considered as a relatively low cost solution for the detection, identification and traceability of fungal bio-inoculants in soil. PMID:26975931

  19. Virulence of Beauveria bassiana Spores Isolated from Larvae of the Potato Tuber Moth,Phthorimaea operculella,Against Myzus persicae%侵染马铃薯块茎蛾幼虫的球孢白僵菌对桃蚜的毒力测定

    Institute of Scientific and Technical Information of China (English)

    何恒果; 李正跃; 陈斌; 计坤

    2004-01-01

    室内测定了从罹病马铃薯块茎蛾Phthorimaea operculella (Zeller)幼虫上分离到的球孢白僵菌Beauveria bassiana两菌株(Bb001,Bb004)对甘蓝桃蚜Myzus persicae (Sulzer)的毒力.运用时间-剂量-死亡率模型,对死亡率随时间和剂量的变化趋势,以及两种菌株在不同温度下对桃蚜的毒力作了分析.结果表明:两种菌株对桃蚜均具有毒杀作用,菌株Bb004在21℃下表现了较高的毒力,菌株Bb001在21℃~28℃下对桃蚜均有较强的致病力,21℃~25℃下是两菌株共同发挥毒力最佳的温度.其中,Bb001有较宽的适宜温度范围,而Bb004适宜温度范围较小.

  20. 不同温度下白僵菌Bb04菌株对红火蚁工蚁的致病力%Pathogenicity of strain Bb04 of Beauveria bassiana (Bals) Vuill. to red imported fire ant workers under different temperatures

    Institute of Scientific and Technical Information of China (English)

    刘晓燕; 吕利华; 何余容

    2014-01-01

    Red imported fire ant ( RIFA) , Solenopsis invicta Buren, is one of the most devastating pests in the world . Entomopathengenic fungi have been applied to control many crops insect pests successfully worldwide and may be a promising control for RIFA.The effect of temperature on pathogenicity of strain Bb 04 of Bearveria bassiana, a suitable candidate to the RIFA workers , was studied in the laboratory .[Method]The RIFA workers were infected with two concentrations of B.bassiana spore suspension (1 ×105 conidia· mL-1 and 1 ×108 conidia· mL-1 ) and kept under 17, 21, 25, 29 and 33 ℃ conditions.[Result]The results showed there were significant differences in virulence of Bb 04 to RIFA under different temperatures .When workers of RIFA were treated with 1 ×108 conidia· mL-1 , the total mortality of infected RIFA after 15 days reached as high as 100%under 21℃, 25℃and 29 ℃.Total mortality was 99.36%at 17 ℃and 98.74%at 33 ℃.However, after 15 days with an infection rate of 1 ×105 conidia/mL, total mortalities of treated workers at 17 ℃, 21 ℃, 25 ℃, 29 ℃and 33 ℃were only 16.83%, 29.42%,36.18%, 33.17%and 27.21%, respectively .At 17, 21 and 25 ℃, LT50 values of Bb04 against RIFA decreased from 5.41 at 17℃to 3.88 at 21 ℃and then 2.57 days at 25℃.LT50 increased again to 2.97 at 29℃and 5.11 at 33℃.[Conclusion and significance]25 ℃was the most effective temperature to promote infection of RIFA by Bb 04 and should be applied in both spring and autumn in the field .%【背景】白僵菌是一种应用最广泛的虫生真菌,已被用于工厂化大量生产,可防治多种农林害虫。【方法】试验设置17、21、25、29和33℃5个温度水平,采用喷雾法,将浓度为1×105和1×108个· mL-1的白僵菌孢子悬浮液分别感染红火蚁工蚁,以测试不同温度下白僵菌菌株对红火蚁工蚁的致病力。【结果】当浓度为1×108个· mL-1时,在21、25和29℃条件下,红火蚁工蚁在15 d

  1. 75 FR 10186 - Beauveria bassiana HF23; Amendment of Exemption from the Requirement of a Tolerance

    Science.gov (United States)

    2010-03-05

    ... the active ingredient for chicken manure treatment in the Federal Register on January 10, 2007 (72 FR..., poultry, eggs, or other products derived from chickens or livestock present in such facilities. In... meat, milk, poultry, eggs, or other products derived from chickens or livestock, the Agency...

  2. Earthworms as phoretic hosts for Steinernema carpocapsae and Beauveria bassiana: Implications for enhanced biological control

    Science.gov (United States)

    Prior research indicated that earthworms may serve as phoretic hosts to entomopathogenic nematodes. Therefore, we hypothesized that biocontrol efficacy of nematodes could be enhanced in the presence of earthworms based on increased nematode dispersal through the soil. We also hypothesized that ear...

  3. Fitness consequences of larval exposure to Beauveria bassiana on adults of the malaria vector Anopheles stephensi

    NARCIS (Netherlands)

    Vogels, C.B.F.; Bukhari, T.; Koenraadt, C.J.M.

    2014-01-01

    Entomopathogenic fungi have shown to be effective in biological control of both larval and adult stages of malaria mosquitoes. However, a small fraction of mosquitoes is still able to emerge after treatment with fungus during the larval stage. It remains unclear whether fitness of these adults is af

  4. A multidisciplinary approach to study virulence of the entomopathogenic fungus Beauveria bassiana towards malaria mosquitoes

    NARCIS (Netherlands)

    Valero Jimenez, C.A.

    2016-01-01

    Although globally malaria mortality rates have fallen by 48% between 2000 and 2015, malaria is still killing an estimated 438,000 people each year. An effective way to alleviate the burden of malaria is to control its vector (malaria mosquitoes) using insecticides. Th

  5. 烟粉虱血细胞对白僵菌的防御反应%Defense Response of Hemolymph in Bemisia tabaci to Beauveria bessiana

    Institute of Scientific and Technical Information of China (English)

    任洁; 韩雪梅; 刘昭; 雷仲仁

    2013-01-01

    To identify the defense mechanism of Bemisia tabaci hemolymph against Beauveria bassiana,cellular immune responses of the infected 3rd instar nymph was investigated with optics microscope and Giemsa stain.The results showed that the invasion of Beauveria bassiana could bring about changes in the total numbers of host hemocytes.After 36 hours inoculation,the blastospores appeared in B. tabaci and the total numbers of host hemocytes were increased.At the same time, the blastospores were adsorbed to the surface of plamatocyte.60 hours after inoculation,the hyphal bodies were absorbed to the surface of granulocytes.More hyphal bodies were observed to the surface of granulocytes 96 hours after inoculation,and the insect body color had appeared the symptoms of infection,which means that the B. bassiana has broken the defense of B. tabaci.B. tabaci can only inhibit the invasion of B. basiana temporarily.%  为了探讨烟粉虱(Bemisia tabaci)血淋巴对白僵菌(Beauveria bassiana)的防御机制,利用吉姆萨染色法在光学显微镜下观察烟粉虱3龄若虫血细胞感染白僵菌后的免疫反应。结果表明:白僵菌的入侵可导致烟粉虱血细胞数量发生改变。体表接种36 h后,虫体内出现芽生孢子,血细胞数量开始增加,芽生孢子被吸附到浆血细胞表面;接种60 h后菌丝段吸附到粒血细胞表面;接种96 h后粒血细胞表面吸附大量菌丝段,此时昆虫体色已经呈现感染症状,说明真菌已经打破了烟粉虱的防御体系,烟粉虱幼虫血淋巴对白僵菌有短时间抑制。

  6. SCREENING OF HIGHER CHITOSANASE-PRODUCING MUTANT OF BEAUVERIA BASSLANA%球孢白僵菌高壳聚糖酶突变株的筛选

    Institute of Scientific and Technical Information of China (English)

    方祥年; 杜昱光; 黄秀梨; 洪洓

    2001-01-01

    以球孢白僵菌1316为出发菌株,通过紫外线和高能电子束分别诱变分生孢子,采用平板透明图法初筛和摇瓶培养复筛的方法,经3轮诱变,筛选到5株高产突变株.其中一株最高产的定名为Beauveria bassiana 1316-V1,其壳聚糖酶活力是原始菌株的16倍;而且经传代培养,其高产特性能够稳定遗传.

  7. PATHOGENICITY OF BEAUVERIA BASSIANA AND APPLICATION OF IT IN THE MANAGEMENT TO THE SOIL-PEST%白僵菌(Beauveria bassiana)的致病性及其在地下害虫防治中的应用

    Institute of Scientific and Technical Information of China (English)

    于有志; 孙洪儒

    2003-01-01

    白僵菌是一种具有潜力的微生物杀虫剂,其杀虫作用受到环境因子、菌株和害虫自身因素等的影响.在土壤环境中应用白僵菌防治害虫,应从白僵菌侵染害虫的途径和方式入手,用特定害虫对白僵菌进行专化培养,选育专一性强的菌株,并与其它试剂混合使用,才能达到预期的效果.

  8. A new record of Beauveria isolated from Scolytidae in China%分离自小蠹虫的白僵菌属一中国新记录种

    Institute of Scientific and Technical Information of China (English)

    黄勃; 陈名君; 王四宝; 李增智; 樊美珍

    2007-01-01

    白僵菌属Beauveria也称波氏菌属,是Vuillemin在1912年为纪念法国真菌学家Beauverie而建立的,它是约100属虫生真菌中最常见和最重要的属之一(蒲蛰龙和李增智,1996).作为真菌杀虫剂的球孢白僵菌Beauveria bassiana (Bals.-Criv.) Vuill.和布氏白僵菌B. brongniartii (Sacc.) Petch在持续控制害虫和维护生态平衡方面具不可替代的作用,因而一直受到国内外生物防治界的关注.

  9. Development of a CO2 releasing co-formulation 1 based on starch, Saccharomyces cerevisiae and Beauveria bassiana attractive towards western corn rootworm larvae

    Science.gov (United States)

    CO2 is known as an attractant for many soil-dwelling pests. To implement an attract-and-kill strategy for soil pest control, CO2 emitting formulations need to be developed. This work aimed at the development of a slow release bead system in order to bridge the gap between application and hatching of...

  10. The infectivity of the entomopathogenic fungus Beauveria bassiana to insecticide-resistant and susceptible Anopheles arabiensis mosquitoes at two different temperatures

    NARCIS (Netherlands)

    Kikankie, C.K.; Brooke, B.D.; Knols, B.G.J.; Koekemoer, L.L.; Farenhorst, M.; Hunt, R.H.; Thomas, M.B.; Coetzee, M.

    2010-01-01

    BACKGROUND: Control of the major African malaria vector species continues to rely extensively on the application of residual insecticides through indoor house spraying or bed net impregnation. Insecticide resistance is undermining the sustainability of these control strategies. Alternatives to the c

  11. The entomopathogenic fungus Beauveria bassiana reduces instantaneous blood feeding in wild multi-insecticide-resistant Culex quinquefasciatus mosquitoes in Benin, West Africa

    NARCIS (Netherlands)

    A.F.V. Howard; R. N'Guessan; C.J.M. Koenraadt; A. Asidi; M. Farenhorst; M. Akogbeto; M.B. Thomas; B.G.J. Knols; W. Takken

    2010-01-01

    Background: Mosquito-borne diseases are still a major health risk in many developing countries, and the emergence of multi-insecticide-resistant mosquitoes is threatening the future of vector control. Therefore, new tools that can manage resistant mosquitoes are required. Laboratory studies show tha

  12. Caracterización molecular de 15 aislamientos de Beauveria bassiana asociados con Cosmopolites y Metamasius en plátano y banano en tres regiones de Colombia

    Directory of Open Access Journals (Sweden)

    Muñoz Flórez Jaime Eduardo

    2008-09-01

    Full Text Available Se colectaron picudos de Cosmopolites y Metamasius en municipios del Valle del Cauca, Caldas y Quindío. Se obtuvieron cultivos monospóricos con diluciones de 10-10 y 10-11. Los aislamientos fueron almacenados a -80°C con glicerol al 10% y el ADN a –20°C. Los marcadores moleculares RAM generaron 82 fragmentos de los cuales 67% fueron polimórficos con una heterocigocidad de 0.24, que indica diversidad media a alta. A un índice de similitud 0.84 se formaron 5 grupos: uno con 11 aislamientos y 4 con un solo aislamiento. En el gran grupo se detectó un duplicado y se encontró diversidad del hongo en los sitios muestreados. No se encontró relación entre aislamientos sobre Cosmopolites y Metamasius o zona geográfica en la formación de grupos genéticos.

  13. The entomopathogenic fungus Beauveria bassiana reduces instantaneous blood feeding in wild multi-insecticide-resistant Culex quinquefasciatus mosquitoes in Benin, West Africa

    NARCIS (Netherlands)

    Howard, A.F.V.; N'Guessan, R.; Koenraadt, C.J.M.; Asidi, A.; Farenhorst, M.; Akogbéto, M.; Thomas, M.B.; Knols, B.G.J.; Takken, W.

    2010-01-01

    Background Mosquito-borne diseases are still a major health risk in many developing countries, and the emergence of multi-insecticide-resistant mosquitoes is threatening the future of vector control. Therefore, new tools that can manage resistant mosquitoes are required. Laboratory studies show that

  14. Metabolic profiles and free radical scavenging activity of Cordyceps bassiana fruiting bodies according to developmental stage.

    Directory of Open Access Journals (Sweden)

    Sun-Hee Hyun

    Full Text Available The metabolic profiles of Cordyceps bassiana according to fruiting body developmental stage were investigated using gas chromatography-mass spectrometry. We were able to detect 62 metabolites, including 48 metabolites from 70% methanol extracts and 14 metabolites from 100% n-hexane extracts. These metabolites were classified as alcohols, amino acids, organic acids, phosphoric acids, purine nucleosides and bases, sugars, saturated fatty acids, unsaturated fatty acids, or fatty amides. Significant changes in metabolite levels were found according to developmental stage. Relative levels of amino acids, purine nucleosides, and sugars were higher in development stage 3 than in the other stages. Among the amino acids, valine, isoleucine, lysine, histidine, glutamine, and aspartic acid, which are associated with ABC transporters and aminoacyl-tRNA biosynthesis, also showed higher levels in stage 3 samples. The free radical scavenging activities, which were significantly higher in stage 3 than in the other stages, showed a positive correlation with purine nucleoside metabolites such as adenosine, guanosine, and inosine. These results not only show metabolic profiles, but also suggest the metabolic pathways associated with fruiting body development stages in cultivated C. bassiana.

  15. Evolutionary diversification of the lizard genus Bassiana (Scincidae across Southern Australia.

    Directory of Open Access Journals (Sweden)

    Sylvain Dubey

    Full Text Available BACKGROUND: Relatively recent (Plio-Pleistocene climatic variations had strong impacts on the fauna and flora of temperate-zone North America and Europe; genetic analyses suggest that many lineages were restricted to unglaciated refuges during this time, and have expanded their ranges since then. Temperate-zone Australia experienced less severe glaciation, suggesting that patterns of genetic structure among species may reflect older (aridity-driven divergence events rather than Plio-Pleistocene (thermally-mediated divergences. The lizard genus Bassiana (Squamata, Scincidae contains three species that occur across a wide area of southern Australia (including Tasmania, rendering them ideally-suited to studies on the impact of past climatic fluctuations. METHODOLOGY/PRINCIPAL FINDINGS: We performed molecular phylogenetic and dating analyses using two partial mitochondrial genes (ND2 and ND4 of 97 samples of Bassiana spp. Our results reveal a pattern of diversification beginning in the Middle Miocene, with intraspecific diversification arising from 5.7 to 1.7 million years ago in the Upper Miocene-Lower Pleistocene. CONCLUSIONS/SIGNIFICANCE: In contrast to the temperate-zone Northern Hemisphere biota, patterns of evolutionary diversification within southern Australian taxa appear to reflect geologically ancient events, mostly relating to east-west discontinuities imposed by aridity rather than (as is the case in Europe and North America relatively recent recolonisation of northern regions from unglaciated refugia to the south.

  16. Biophysicochemical characterization of an alkaline protease from Beauveria sp. MTCC 5184 with multiple applications.

    Science.gov (United States)

    Shankar, Shiv; Laxman, Ryali Seeta

    2015-01-01

    This study illustrates the biophysicochemical properties of an alkaline protease, BAP (Beauveria sp. alkaline protease) from Beauveria sp. MTCC 5184. This protease exhibited maximum activity at 50 °C, pH 9.0, and stability in a broad pH range, in the presence of organic solvents, denaturants, as well as detergents. Wash performance studies revealed that BAP was able to remove blood clots/stains from blood-soaked cloth. Peptide mass fingerprinting results demonstrated partial homology of BAP with subtilisin-like proteinase. BAP showed catalytic activity against natural as well as synthetic substrates. Active site characterization of BAP confirmed the involvement of serine, tryptophan, and aspartic acid in catalytic activity. Detailed kinetic and thermodynamic studies of BAP demonstrated that the activation energy (Ea) for casein hydrolysis was 82.55 kJ/M, the specificity constant (Kcat/K m), and the values of ∆G (change in Gibbs free energy) decreased with increase in temperature, whereas ∆H (change in enthalapy) and ∆S (change in entropy) were constant. The results of the present study indicate that BAP has potential for applications as detergent additive, in peptide synthesis, and in basic research.

  17. Novel antimicrobial cyclodepsipeptides from the culture broth Fungu Beauveria felina%Beauveria feline培养物中的新的cyclodepsipeptide类化合物

    Institute of Scientific and Technical Information of China (English)

    廖志新; 岳建民

    2003-01-01

    从微生物(Beauveria felina)的发酵液的乙酸乙酯萃取物中分离得到8个结构新颖的cyclodepsipeptide类化合物,经光谱(尤其是1D、2D NMR及MS)及化学方法鉴定了所有的8个化合物的结构,结果表明1-5为新的化合物,命名为felinains A-E,3个已知化合物分别鉴定为Antibiotic M6124,isarin及roseocardin.通过手性HPLC技术对化合物水解所得氨基酸构型的测定,确定了5个新化合物的绝对构型,并对所有化合物进行了生物学活性测试.

  18. Optimization of cyclosporin A production by Beauveria nivea in continuous fed-batch fermentation

    Directory of Open Access Journals (Sweden)

    Dong Huijun

    2011-01-01

    Full Text Available To develop the effective control method for fed-batch culture of cyclosporin A production, we chose fructose, L-valine and (NH42HPO4 as feeding nutrients and compared their productivities in relation to different concentrations. The feeding rate of three kinds of feeding materials was controlled to maintain the suitable residual concentration. The fed-batch fermentation results indicated that the optimal concentrations of fructose, L-valine and (NH42HPO4 were about 20 g/L, 0.5 g/L and 0.6 g/L for cyclosporin A production, respectively. The cultivation of Beauveria nivea could produce cyclosporin A up to 6.2 g/L for 240 hrs through a continuous feeding-rate-controlled-batch process under the optimal feeding conditions.

  19. Drug: D09176 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available 91], Beauveria bassiana [TAX:176275] Same as: E00308 Bombycidae Silkworm infected Cordycepitaceae Beauveria bassiana Vuillemin.; Stan...dards for non-pharmacopoeial crude drugs Crude drugs [BR:br08305] Animals Insects D09176 Stiff silkworm PubChem: 96025856 ...

  20. Beauveria brongniartii Sacc. (Petch) against Melolontha spp. white grubs in forest nurseries with different soil pH

    OpenAIRE

    Sierpińska Alicja; Popowska-Nowak Elżbieta; Bednarek Andrzej

    2015-01-01

    In 2011, the General Directorate of State Forests in Poland managed 669 forest nurseries on the total area of 2411 ha that produced forest tree seedlings bare root systems, hence vulnerable to Melolontha spp. white grubs. Up to date, no chemical or biological plant protection product for control of cockchafer grubs in forests has been registered in Poland. The study was carried out with the aim to evaluate the efficacy of a biocontrol product Bovecol with BP strain of Beauveria brongniartii o...

  1. 亚洲玉米螟优良球孢白僵菌菌株的筛选%Screening of Superior Strain of Beauveria bassiana Parasitized on Ostrinia furnacalis

    Institute of Scientific and Technical Information of China (English)

    徐艳聆; 吕文彦; 杜开书; 王蕊; 张萌萌

    2010-01-01

    亚洲玉米螟是中国玉米生产上最重要的害虫,对玉米的产量和质量影响很大.为了探索对其无公害的防治方法,筛选出高毒力的生防真菌,试验选择从亚洲玉米螟和桃蛀螟僵虫中分离纯化到的5个球孢白僵菌野生菌株,进行了毒力、菌株生长速率、产孢量和孢子萌发率的测定,筛选出了2株高毒力菌株B6和B17,在1×108孢子/mL,浓度下对亚洲玉米螟的致死率分别为95.4%和90.8%,僵虫率分别为94.4%和87.8%,LT50分别为4.4天和4.7天;其LC50分别为1.3×106孢子/mL,和2.8×106孢子/mL,并且这2株菌株显现出良好的生物学特性.结果表明,B6和B17菌株致病力强、产孢量大、萌发率高,具有较大的生产潜力.

  2. Change of Activity of Phenoloxidase and Hemocytes in Apriona germari Larvae Infected by Beauveria bassiana%白僵菌侵染对桑天牛幼虫酚氧化酶活性和血细胞的影响

    Institute of Scientific and Technical Information of China (English)

    李会平; 黄大庄; 苏筱雨; 黄秋娴

    2009-01-01

    桑天牛(Apriona germari)是多种林木、果树、花卉的重要蛀干害虫,也是我国危害最严重、最难防治的害虫之一。近年来,其猖獗危害已成为我国养蚕业、林业及果树种植业等经济发展的严重障碍。白僵菌(Beauveria bassiana)是重要的昆虫病原微生物,

  3. A multi-screening approach for marine-derived fungal metabolites and the isolation of cyclodepsipeptides from Beauveria felina

    International Nuclear Information System (INIS)

    Extracts obtained from 57 marine-derived fungal strains were analyzed by HPLC-PDA, TLC and 1H NMR. The analyses showed that the growth conditions affected the chemical profile of crude extracts. Furthermore, the majority of fungal strains which produced either bioactive of chemically distinctive crude extracts have been isolated from sediments or marine algae. The chemical investigation of the antimycobacterial and cytotoxic crude extract obtained from two strains of the fungus Beauveria felina have yielded cyclodepsipeptides related to destruxins. The present approach constitutes a valuable tool for the selection of fungal strains that produce chemically interesting or biologically active secondary metabolites. (author)

  4. Cyclodepsipeptides and Other O-Containing Heterocyclic Metabolites from Beauveria felina EN-135, a Marine-Derived Entomopathogenic Fungus

    Directory of Open Access Journals (Sweden)

    Feng-Yu Du

    2014-05-01

    Full Text Available Bioassay-guided fractionation of a culture extract of Beauveria felina EN-135, an entomopathogenic fungus isolated from a marine bryozoan, led to the isolation of a new cyclodepsipeptide, iso-isariin D (1; two new O-containing heterocyclic compounds that we have named felinones A and B (2 and 3; and four known cyclodepsipeptides (4–7. The structures were elucidated via spectroscopic analysis, and the absolute configurations of 1 and 2 were determined using single-crystal X-ray diffraction and CD, respectively. All isolated compounds were evaluated for antimicrobial activity and brine-shrimp (Artemia salina lethality.

  5. A multi-screening approach for marine-derived fungal metabolites and the isolation of cyclodepsipeptides from Beauveria felina

    Energy Technology Data Exchange (ETDEWEB)

    Vita-Marques, Aline Maria de; Lira, Simone P.; Berlinck, Roberto G.S. [Universidade de Sao Paulo (USP), Sao Carlos, SP (Brazil). Inst. de Quimica]. E-mail: rgsberlinck@iqsc.usp.br; Seleghim, Mirna H.R. [Universidade Federal de Sao Carlos (UFSCAR), SP (Brazil). Dept. de Ecologia e Biologia Evolutiva; Sponchiado, Sandra R.P. [UNESP, Araraquara, SP (Brazil). Inst. de Quimica. Dept. de Bioquimica e Tecnologia Quimica; Tauk-Tornisielo, Samia M. [UNESP, Rio Claro, SP (Brazil). Centro de Estudos Ambientais; Barata, Margarida [Faculdade de Ciencias de Lisboa (Portugal). Centro de Biologia Ambiental. Dept. de Biologia Vegetal; Pessoa, Claudia; Moraes, Manoel O. de; Cavalcanti, Bruno Coelho [Universidade Federal do Ceara (UFC), Fortaleza, CE (Brazil). Dept. de Fisiologia e Farmacologia; Nascimento, Gislene G.F. [Universidade Metodista de Piracicaba (UNIMEP), SP (Brazil). Faculdade de Ciencias da Saude; Souza, Ana O. de [Instituto Butantan, Sao Paulo, SP (Brazil); Galetti, Fabio C.S.; Silva, Celio L. [Universidade de Sao Paulo (USP), Ribeirao Preto, SP (Brazil). Faculdade de Medicina. Dept. de Bioquimica e Imunologia; Silva, Marcio; Pimenta, Eli F.; Thiemann, Otavio [Universidade de Sao Paulo (USP), Sao Carlos, SP (Brazil). Inst. de Fisica; Passarini, Michel R.Z.; Sette, Lara D. [Universidade Estadual de Campinas (UNICAMP), Campinas, SP (Brazil). Centro Pluridisciplinar de Pesquisas Quimicas, Bioligicas e Agricolas. Div. de Recursos Microbianos

    2008-07-01

    Extracts obtained from 57 marine-derived fungal strains were analyzed by HPLC-PDA, TLC and {sup 1}H NMR. The analyses showed that the growth conditions affected the chemical profile of crude extracts. Furthermore, the majority of fungal strains which produced either bioactive of chemically distinctive crude extracts have been isolated from sediments or marine algae. The chemical investigation of the antimycobacterial and cytotoxic crude extract obtained from two strains of the fungus Beauveria felina have yielded cyclodepsipeptides related to destruxins. The present approach constitutes a valuable tool for the selection of fungal strains that produce chemically interesting or biologically active secondary metabolites. (author)

  6. Selection, Identification and Culture Characteristics of a Highly Virulent Strain of Beauveria towards Sympiezomias citri%柑橘灰象甲一株高毒力白僵菌菌株的筛选鉴定及培养特性

    Institute of Scientific and Technical Information of China (English)

    王定锋; 黎健龙; 王庆森; 李慧玲; 吴光远

    2014-01-01

    %, highest muscardine rates of 97.78%and shortest LT50 of 3.77 d after fungal inoculation at a concentration of 1.0×108 spores/mL (7 d, 26 ℃). The LC50 value was 4.55×104 spores/mL at 26 ℃. According to culture characteristics, morphology feature and rDNA ITS sequence analysis, the strain Bb2-1 was identified to be Beauveria bassiana. The effect of five medium types (PDA, PPDA, 1/4 SDAY, SDAY, and Czapek), five temperature gradient (15, 20, 25, 30 and 35 ℃), seven different pH value (pH 4.0—10.0) and seven different illumination periods (0L:24D, 24L:0D, 18L:6D, 12L:12D, 6L:18D, dark in the first 7 days and light in the last 7 days, and light in the first 7 days and dark in the last 7 days) on colony growth and sporulation of the strain Bb2-1 were evaluated through the determination of colony diameter and sporulation. The results showed that SDAY medium at pH 7.0 was the most optimal medium for the colony growth and sporulation of the strain Bb2-1 at 25 ℃, and photoperiod didn’t pose any obvious effects on colony growth, but prolonging illumination time was benefit to the sporulation. In this study, the selection of a highly virulent B. bassiana (Bb2-1) towards S. citri and the evaluation of culture characteristics of Bb2-1 would provide reference for large scale production of the fungi to control this beetle.

  7. Selection and characterization of Beauveria spp. isolates to control the broad mite Polyphagotarsonemus latus (Banks, 1904) (Acari: Tarsonemidae).

    Science.gov (United States)

    Martins, C C; Alves, L F A; Mamprim, A P; Souza, L P A

    2016-01-01

    This study was performed under laboratory conditions to identify isolates of the fungus Beauveria spp. that can control Polyphagotarsonemus latus in the greenhouse and field. Thirty Beauveria spp. isolates were tested by spraying 1 mL conidia (1 × 108 conidia/mL) on pepper leaf discs containing 15 mites. Evaluations were performed on the 3rd and 6th day post application by counting the number of dead mites. Vegetative growth and conidial production were measured from the selected isolates, and bioassays were conducted in the greenhouse on bean seedlings in plastic pots. The isolate Unioeste 53 was selected, and a conidial suspension (1 × 108 conidia/mL) was applied with a backpack sprayer. The evaluation consisted of pre- and post-treatment counts of the number of live mites on ten leaflets in both the plots treated with the fungus and control plots, and the same procedure was followed for the field experiment. In the laboratory, the Unioeste 53 isolate resulted in total and confirmed mortality rates of 70% and 57.7%, respectively. In the greenhouse, the population decreased by 76.71% by the 16th day after application. In the field, the population decreased by 66% by the 12th day after application, demonstrating the potential of this fungus for mite management. PMID:27332672

  8. Entomopathogenic fungi in predatory beetles (Col: Carabidae and Staphylinidae) from agricultural fields

    DEFF Research Database (Denmark)

    Steenberg, T; Langer, V; Esbjerg, P

    1995-01-01

    beetles were low (Carabidae: max. 7.6%, Staphylinidae: max. 7.0%). in comparison, prevalence of entomopathogenic fungi in carabid larvae was high (19-50%). At one study site an epizootic of Beauveria bassiana was observed, infecting 67% of staphylinid Anotylus rugosus and 37% of the staphylinid Gyrohypnus...... angustatus. Beauveria bassiana was the predominant fungus isolated from ground beetles and rove beetles from all studied sites. Other fungal species included the hyphomycetes Metarhizium anisopliae, Paecilomyces farinosus and Verticillium lecanii as well as Zoophthora radicans and Zoophthora philonthi...... (Zygomycetes: Entomophthorales). Two individuals of Anotylus rugosus were found to have a dual infection of Zoophthora philonthi and Beauveria bassiana...

  9. Baeuveria bassiana (BalsVuill and Metarhizium anisioplae (Metsch. Sorokin in the pupas control of Prodiplosis longifila Gagné on asparagus crop

    Directory of Open Access Journals (Sweden)

    Carolina Cedano

    2012-03-01

    Full Text Available The objective of the present work was to evaluate the effect of the entomopathogen fungi Baeuveria bassiana and Metarhizium anisioplae of the Prodiplosis longifila pupas, in order to reduce the adult population of this insect. The treatments consisted on the application of two different propagel concentrations (mycel and conidia of each entomopathogen. One of these concentration was 1x106 propagels/ml (obtained from the total amount of conidia collected of 25 kg of rice colonized by the entomopathogen; and the other one, was 1x107 propagels/ml (obtained from the total amount of conidia collected of 40 kg of rice colonized by the entomopathogen. As a check a non application treatment was considered. The applications were trough the irrigation system and started 15 days after the end of harvest and were repeated each five days during a month, coincident with the period of most falls of pupas of the soil. As a result Baeuveria bassiana at 1x107 propagels/ml shown 53.4 % of the total pupas colonized by the entomopathogen, which allows indicating B. bassiana as a promissory biocontrol of this specie

  10. Efectividad de Aislamientos de Hongos Entomopatógenos Sobre Larvas de Polilla del Tomate Tuta absoluta Meyrick (Lepidoptera: Gelechiidae) Effectivity of Entomopathogenic Fungus Strains on Tomato Moth Tuta absoluta Meyrick (Lepidoptera: Gelechiidae) Larvae

    OpenAIRE

    Marta Rodríguez; Marcos Gerding; Andrés France

    2006-01-01

    La polilla del tomate, Tuta absoluta Meyrick, es una plaga importante en el cultivo del tomate (Lycopersicon esculentum L.) en Chile. Estudios de laboratorio fueron realizados para evaluar la virulencia y efectividad de Qu-M558 de Metarhizium anisopliae var. anisopliae y Qu-B912 de Beauveria bassiana. La primera evaluación fue realizada aplicando concentraciones de 0 a 10(8) conidia mL-1 directamente sobre larvas de tercer estadio mediante el sistema de pulverización torre Potter. Las compara...

  11. Mass production of fungal entomopathogens

    Science.gov (United States)

    Hypocrealean fungi encompassing Beauveria bassiana, B. brongniartii, Isaria fumosorosea, I. farinosa, several Lecanicillium spp., Nomuraea rileyi, and Metarhizium spp. are being increasingly exploited worldwide for insect pest management because of the ease with which they can be produced in contras...

  12. Cotton in Benin: governance and pest management

    NARCIS (Netherlands)

    Togbe, C.E.

    2013-01-01

    Key words: cotton, synthetic pesticides, neem oil (Azadirachta indica), Beauveria bassiana, Bacillus thuringiensis, field experiment, farmers’ participation   Pests are one of the main factors limiting cotton production worldwide. Most of the pest control strategies in cotton producti

  13. Entomopathogenic fungi recorded from the harlequin ladybird, Harmonia axyridis

    DEFF Research Database (Denmark)

    Steenberg, Tove; Harding, Susanne

    2009-01-01

    Entomopathogenic fungi were recorded from field samples of the harlequin ladybird Harmonia axyridis, an invasive coccinellid that has recently arrived in Denmark. Larvae, pupae and adults were found to be infected by Isaria farinosa, Beauveria bassiana and species of Lecanicillium...

  14. Optical and structural properties of plasma-treated Cordyceps bassiana spores as studied by circular dichroism, absorption, and fluorescence spectroscopy

    Science.gov (United States)

    Lee, Geon Joon; Sim, Geon Bo; Choi, Eun Ha; Kwon, Young-Wan; Kim, Jun Young; Jang, Siun; Kim, Seong Hwan

    2015-01-01

    To understand the killing mechanism of fungal spores by plasma treatment, the optical, structural, and biological properties of the insect pathogenic fungus Cordyceps bassiana spores were studied. A nonthermal atmospheric-pressure plasma jet (APPJ) was used to treat the spores in aqueous solution. Optical emission spectra of the APPJ acquired in air indicated emission peaks corresponding to hydroxyl radicals and atomic oxygen. When the APPJ entered the aqueous solution, additional reactive species were derived from the interaction of plasma radicals with the aqueous solution. Fluorescence and absorption spectroscopy confirmed the generation of hydroxyl radicals and hydrogen peroxide in the plasma-activated water (PAW). Spore counting showed that plasma treatment significantly reduced spore viability. Absorption spectroscopy, circular dichroism (CD) spectroscopy, and agarose gel electrophoresis of the DNA extracted from plasma-treated spores showed a reduction in spore DNA content. The magnitude of the dip in the CD spectrum was lower in the plasma-treated spores than in the control, indicating that plasma treatment causes structural modifications and/or damage to cellular components. Tryptophan fluorescence intensity was lower in the plasma-treated spores than in the control, suggesting that plasma treatment modified cell wall proteins. Changes in spore viability and DNA content were attributed to structural modification of the cell wall by reactive species coming from the APPJ and the PAW. Our results provided evidence that the plasma radicals and the derived reactive species play critical roles in fungal spore inactivation.

  15. Optical and structural properties of plasma-treated Cordyceps bassiana spores as studied by circular dichroism, absorption, and fluorescence spectroscopy

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Geon Joon, E-mail: gjlee@kw.ac.kr; Sim, Geon Bo; Choi, Eun Ha [Plasma Bioscience Research Center/Department of Electrical and Biological Physics, Kwangwoon University, Seoul 139-701 (Korea, Republic of); Kwon, Young-Wan [KU-KIST Graduate School of Converging Science and Technology, Korea University, Seoul 136-701 (Korea, Republic of); Kim, Jun Young; Jang, Siun; Kim, Seong Hwan, E-mail: piceae@naver.com [Department of Microbiology and Institute of Basic Sciences, Dankook University, Cheonan 330-714 (Korea, Republic of)

    2015-01-14

    To understand the killing mechanism of fungal spores by plasma treatment, the optical, structural, and biological properties of the insect pathogenic fungus Cordyceps bassiana spores were studied. A nonthermal atmospheric-pressure plasma jet (APPJ) was used to treat the spores in aqueous solution. Optical emission spectra of the APPJ acquired in air indicated emission peaks corresponding to hydroxyl radicals and atomic oxygen. When the APPJ entered the aqueous solution, additional reactive species were derived from the interaction of plasma radicals with the aqueous solution. Fluorescence and absorption spectroscopy confirmed the generation of hydroxyl radicals and hydrogen peroxide in the plasma-activated water (PAW). Spore counting showed that plasma treatment significantly reduced spore viability. Absorption spectroscopy, circular dichroism (CD) spectroscopy, and agarose gel electrophoresis of the DNA extracted from plasma-treated spores showed a reduction in spore DNA content. The magnitude of the dip in the CD spectrum was lower in the plasma-treated spores than in the control, indicating that plasma treatment causes structural modifications and/or damage to cellular components. Tryptophan fluorescence intensity was lower in the plasma-treated spores than in the control, suggesting that plasma treatment modified cell wall proteins. Changes in spore viability and DNA content were attributed to structural modification of the cell wall by reactive species coming from the APPJ and the PAW. Our results provided evidence that the plasma radicals and the derived reactive species play critical roles in fungal spore inactivation.

  16. Antiproliferative and Apoptosis-Inducing Activities of 4-Isopropyl-2,6-bis(1-phenylethylphenol Isolated from Butanol Fraction of Cordyceps bassiana

    Directory of Open Access Journals (Sweden)

    Ji Hye Kim

    2015-01-01

    Full Text Available The Cordyceps species have been widely used for treating various cancer diseases. Although the Cordyceps species have been widely known as an alternative anticancer remedy, which compounds are responsible for their anticancer activity is not fully understood. In this study, therefore, we examined the anticancer activity of 5 isolated compounds derived from the butanol fraction (Cb-BF of Cordyceps bassiana. For this purpose, several cancer cell lines such as C6 glioma, MDA-MB-231, and A549 cells were employed and details of anticancer mechanism were further investigated. Of 5 compounds isolated by activity-guided fractionation from BF of Cb-EE, KTH-13, and 4-isopropyl-2,6-bis(1-phenylethylphenol, Cb-BF was found to be the most potent antiproliferative inhibitor of C6 glioma and MDA-MB-231 cell growth. KTH-13 treatment increased DNA laddering, upregulated the level of Annexin V positive cells, and altered morphological changes of C6 glioma and MDA-MB-231 cells. In addition, KTH-13 increased the levels of caspase 3, caspase 7, and caspase 9 cleaved forms as well as the protein level of Bax but not Bcl-2. It was also found that the phosphorylation of AKT and p85/PI3K was also clearly reduced by KTH-13 exposure. Therefore, our results suggest KTH-13 can act as a potent antiproliferative and apoptosis-inducing component from Cordyceps bassiana, contributing to the anticancer activity of this mushroom.

  17. Beauveria brongniartii Sacc. (Petch against Melolontha spp. white grubs in forest nurseries with different soil pH

    Directory of Open Access Journals (Sweden)

    Sierpińska Alicja

    2015-12-01

    Full Text Available In 2011, the General Directorate of State Forests in Poland managed 669 forest nurseries on the total area of 2411 ha that produced forest tree seedlings bare root systems, hence vulnerable to Melolontha spp. white grubs. Up to date, no chemical or biological plant protection product for control of cockchafer grubs in forests has been registered in Poland. The study was carried out with the aim to evaluate the efficacy of a biocontrol product Bovecol with BP strain of Beauveria brongniartii on sterilized wheat grain in control of Melolontha spp. white grubs in forest nurseries, established on acidic soils (with pH analogous to that of forest soils as well as on those alkaline. The active substance of Bovecol was 108 fungal conidia per 1g of the product. Study plots were established in 3 bareroot nurseries, situated in the areas with different soil reaction values and abundant populations of Melolontha spp. white grubs (assessed before Bovecol treatments. The product was applied against L1 larvae (rates in the Czerniawka and Bałtów nurseries: 120 kg/ha and 240 kg/ha and against L2 larvae (the Iłki nursery: 240 kg/ha. Grub population numbers were assessed 2, 3 and 4 months after the treatments. Application of Bovecol against L1 into the soil with neutral reaction (pH 6.8 caused the reduction of white grub numbers in 2 months, down to the threshold recommended by the Instruction of the protection of forests (mandatory guidelines for the protection of Poland’s State Forests, i.e. less than 1 grub/sampling pit. Bovecol treatment against L1 cockchafer larvae into acidic soil (pH 4.8 had no statistically significant effect on the reduction of grub population numbers, even 4 months after product application. Bovecol treatment against L2 larvae applied into the soil with medium pH value (5.3 gave poorer results when compared to the soil with pH 6.8, but considerably better – when compared to the soil with pH 4.8. The selection of a fungal strain

  18. The effect of Beauveria brongniartii and its secondary metabolites on the detoxification enzymes of the pine caterpillar, Dendrolimus tabulaeformis.

    Science.gov (United States)

    Fan, Jinhua; Xie, Yingping; Xue, Jiaoliang; Liu, Rui

    2013-01-01

    The mortality of pine caterpillar, Dendrolimus tabulaeformis Tsai et Liu (Lepidoptera: Lasiocampidae), larvae treated with Beauveria brongniartii (Saccardo) Petch (Hypocreales: Clavicipitaceae) conidia and cell-free culture supernatants enriched for the secondary metabolites of the fungus was investigated. In addition, the effects of the treatments on the activities of two insect-related defense response proteins, glutathione S-transferase (GST) and esterase (EST), were measured over time. Bioassays were performed using a range of fungal spore (6 × 10⁵ through 6 × 10⁷ spores/mL) and supernatant extract concentrations (5.5-550 µg/mL). The results showed that the mortalities of D. tabulaeformis larvae were closely related to the concentration of the conidia and the metabolites of B. brongniartii. The differences among the treatments all reached a significant level. The activities of the two detoxifying enzymes, GST and EST, in the larvae increased simultaneously post-treatment. After infection with the conidial suspensions, the highest GST activity appeared at 3 days, and the activities of the caterpillars infected with 6 × 10⁶ spores/mL and 6 × 10⁷ spores/mL were significantly higher than in the control. Using α-naphthyl, the highest activity of EST also appeared at 3 days, and the differences for the three different concentrations were significant. A similar trend of change in the EST activity was observed using β-naphthyl. After treatment with the secondary metabolite solution, the highest GST activity appeared at 6 hr, and significant differences were found both for the different durations (2, 4, 6, 12, 24, and 48 hr) and in the three concentration groups. When using α-naphthyl, the EST activity peak appeared at 24 hr, and the differences were significant among the durations of 2, 4, 6, 12, 24, and 48 hr. The effect of the concentration of the secondary metabolite solution notably induced the EST activity in the insects, and a similar result was

  19. Ecological aspects of a Cosmopolites sordidus (Germar (Coleoptera: Curculionidae population in São Miguel do Iguaçu, PR./ Aspectos ecológicos da população de Cosmopolites sordidus, (Germar (Coleoptera: Curculionidae em São Miguel do Iguaçu, PR

    Directory of Open Access Journals (Sweden)

    Cristiane Rohde

    2006-06-01

    Full Text Available The population dynamics of Cosmopolites sordidus (Germar was studied in banana crops (cultivar Nanicão at São Miguel do Iguaçu, PR, using roof-tile-like baits, between June/2003 and May/2004. Adults infected by entomopathogenic fungi were recorded, and predators were also found on the baits. A comparative bioassay was carried out between the Beauveria bassiana isolate obtained from the experiment area and from other regions in Brazil against C. sordidus adults. The population peaks of C. sordidus adults occurred in June and July, a period with mild temperatures and low precipitation. The population of C. sordidus larvae, however, was concentrated in December. The insect predators found in the area were insects of the following orders: Coleoptera (Carabidae, Dermaptera (Forficulidae, Hemiptera (Reduviidae, Hymenoptera (Formicidae, and spiders of the families Ctenidae, Clubionidae, and Lycosidae, with a population peak two months after the pest population peak. A low number of C. sordidus adults infected by B. bassiana was found, with an annual mean of 0.44%. In the laboratory, the B. bassiana isolates were pathogenic to the insects.Estudou-se a dinâmica populacional de Cosmopolites sordidus (Germar em cultivo de banana(cultivar Nanicão, em São Miguel do Iguaçu, PR, através de iscas do tipo telha, entre junho/2003 a maio/2004. Atribuiu-se conceito ao estado de conservação da isca, visando observar sua durabilidade e atratividade. Registraram-se adultos infectados por fungos entomopatogênicos, sendo também encontrados predadores nas iscas. Realizou-se um bioensaio com isolados de Beauveria bassiana da área experimental e de Centros de Pesquisa do Brasil, para avaliar a patogenicidade em C. sordidus. Verificou-se que os picos populacionais de adultos de C. sordidus ocorreram em junho e julho, período de temperaturas amenas e baixas precipitações, enquanto que as larvas foram mais abundantes em dezembro. Os insetos predadores

  20. Cloning and sequence analysis of cDNA and upstream sequence of Bbhsp70 gene from Beauveria bassiana%球孢白僵菌热休克蛋白基因Bbhsp70的cDNA及上游序列克隆与分析

    Institute of Scientific and Technical Information of China (English)

    谢翎; 陈红梅; 蒲顺昌; 汤强; 李增智; 黄勃

    2009-01-01

    运用SMART RACE RT-PCR技术与DNA步移技术,首次从球孢白僵菌中克隆出完整的热休克蛋白基因Bbhsp70编码区序列及上游序列.该基因cDNA全长2405bp,5'端非翻译区171bp,3'端非翻译区263bp,开放阅读框(ORF)1971bp,编码656个氨基酸.成熟蛋白理论分子量为71.3kDa,理论等电点为4.92.上游序列长度3559bp,其中有305bp序列与cDNA序列重叠.分析表明,上游序列中没有明显的TAYA-盒和CAAT-盒,但含有CCAAT-binding factor、GC-box等重要的转录因子结合位点,以及热激应答元件(HSE)和GATA元件等启动子顺式调控元件.

  1. CLONING AND CHARACTERIZATION OF A HOMOLOGOUS GENE OF YEAST FUS3/KSS1 MAPK FROM BEAUVERIA BASSIANA%球孢白僵菌FUS3/KSS1类MAPK同源基因(BbMPK1)的克隆及特征分析

    Institute of Scientific and Technical Information of China (English)

    张永军; 方卫国; 金凯; 罗志兵; 周永洪; 肖月华; 裴炎

    2005-01-01

    根据几种丝状真菌FUS3/KSS1类MAPK的保守序列设计简并引物,从昆虫病原真菌球孢白僵菌中扩增出MAPK基因的部分片段,进而利用YADE法延伸该片段的上、下游邻接序列,获得MAPK基因的全长序列,命名为BbMPK1.用3( RACE扩增出BbMPK1的全长cDNA序列,该序列含有一个1071bp的ORF,编码356个氨基酸的多肽,推测分子量为41.2 kDa,等电点为6.61.BbMPK1含有11个MAPK共有的蛋白激酶区域和1个MAPK激酶作用的磷酸化位点区域(TEY),其氨基酸序列与丝状真菌的TMKA、PMK1、CMK1、FMK1和BMP1等MAPK高度同源.系统聚类结果表明,BbMPK1属于酵母FUS3/KSS1类MAPK.Southern杂交表明,BbMPK1在球孢白僵菌基因组中以单拷贝形式存在.RT-PCR分析表明BbMPK1在分生孢子休眠阶段、萌发阶段和菌丝生长时期均表达.研究结果为阐明酵母FUS3/KSS1类MAPK同源基因在球孢白僵菌中的作用奠定了基础.

  2. para palmito

    Directory of Open Access Journals (Sweden)

    Francisco Paulo Chaimsohn

    2007-01-01

    Full Text Available Densidades de siembra, arreglos espaciales y fertilización en pejibaye (Bactris gasipaes cv Diamantes-10 para palmito. La investigación se llevó a cabo en la Estación Experimental Los Diamantes (Guápiles, Costa Rica, el 3 de octubre del 2003, cuyo objetivo fue la evaluación del efecto de diferentes densidades de siembra (3.333, 5.000 y 6.666 plantas/ha, arreglos espaciales, y diversos métodos de fertilización (química, orgánica, sobre el crecimiento de las plantas de pejibaye para producción de palmito. Se consideraron las variables diámetro y altura del tallo primario y el número de hojas y rebrotes como indicadores de producción. El período de evolución abarcó sólo los primeros 25 meses de crecimiento en el campo. El número de hojas, la altura y el diámetro del tallo no mostraron diferencias de respuesta relevantes. Sólo el número de rebrotes disminuyó al aumentar la densidad de la población, cuando se midió a los 15 meses de edad. El efecto de la fertilización se hizo evidente después de la primera cosecha, realizada a los 20 meses, debido al aumento de la competencia entre plantas, ahora más desarrolladas. Fue entonces cuando la fertilización química indujo la producción de un mayor número y vigor de los rebrotes. Sin embargo, las prácticas evaluadas 25 meses después de la siembra, no habían infl uido hasta ese momento en el número de palmitos cosechados, ni tampoco había afectado las características físicas de los sectores foliar y caulinar del palmito.

  3. Para Leer

    Directory of Open Access Journals (Sweden)

    Adela Sanz Cañibano

    2012-01-01

    Full Text Available Jorge L. Tizón, Ramón Ciurana, M del Carmen Fernández (Comps. Libro de casos. Promoción de la salud mental desde la atención primaria. (Adela Sanz Cañibano Grupo de Expertos en Competencia Intercultural. Instrumento para la valoración de la Competencia Intercultural en la Atención en salud mental. Hacia la equidad en salud. (María Goretti Morón Nozaleda Mariano Hernández Monsalve y Pilar Nieto Degregori (Coords.. Psicoterapia y Rehabilitación de pacientes con psicosis. (Francisco Chicharro Lezcano André du Laurens. De las enfermedades melancólicas. Jourdain Guibelet. Del humor melancólico. (Iñaki Markez Julio José? Segarra Valls. Léxico psico(patológico en la obra de Carlos Castilla del Pino. (Eudoxia Gay Pamos Giorgio Nardone y Alessandro Salvini. El diálogo estratégico. (Fernando Mansilla Izquierdo Michel Foucault. Leçons sur la volonté de savoir, suivi de Le savoir d'Edipe. (Mauricio Jalón Camilo Castelo Branco. La novela de un hombre rico. (Alicia Merisi Kenzaburo Oé. Cuadernos de Hiroshima. (Mauricio Jalón Pascal Quignard. Les solidarités mystérieuses. (Beatriz A. Rubín Documentos en la red (Juan Medrano

  4. Compatibility of Encarsia formosa (Hymenoptera: Aphelinidae with Commercial Products Under Laboratory Conditions

    Directory of Open Access Journals (Sweden)

    Jennifer Barrera Mojica

    2013-04-01

    Full Text Available The greenhouse white fly, Trialeurodes vaporariorum one of the major pests of tomatoes under greenhouse conditions, but, Its mainly controlled with chemical insecticides. However, there are alternative control strategies as entomopathogens fungi and parasitoids (Encarsia formosa. In this study we evaluated the compatibility of commercial product with adults and pupae of E. formosa under laboratory conditions. Eight treatments were evaluated for adults and pupae of the parasitoid, including two concentrations of the entomopathogenic fungus Beauveria bassiana (5 x 107 and 5 x 109 conidia/ml, commercial doses of four chemical products commonly used to control pests on tomato crops and two controls. These treatments were applied directly on parasitoids, and their mortality rate on adults and pupae were recorded daily. There was no difference in E. formosa pupae among relative with the Bacillus thuringiensis products (p > 0.05, indicating that its compatible with the parasitoid pupae under laboratory conditions. Finally, it was found that products with active ingredients of Tiocyclam hidrogenoxalato and B. thuringiensis are incompatibles with E. formosa adults under laboratory conditions.COMPATIBILIDAD DE Encarsia formosa (HYMENOPTERA: APHELINIDAE CON PRODUCTOS COMERCIALES EN CONDICIONES DE LABORATORIO La mosca blanca de los invernaderos, Trialeurodes vaporariorum, es una de las principales plagas de cultivos de tomate bajo invernadero y es controlada principalmente con insecticidas químicos. Sin embargo, existen estrategias alternativas de control como hongos entomopatógenos (Beauveria bassiana y enemigos naturales como parasitoides (Encarsia formosa. En el presente estudio se evaluó la compatibilidad de productos comerciales con adultos y pupas de E. formosa bajo condiciones de laboratorio. Se evaluaron ocho tratamientos para pupas y adultos del parasitoide, incluyendo dos concentraciones del hongo entomopatógeno Beauveria

  5. 4-Isopropyl-2,6-bis(1-phenylethylaniline 1, an Analogue of KTH-13 Isolated from Cordyceps bassiana, Inhibits the NF-κB-Mediated Inflammatory Response

    Directory of Open Access Journals (Sweden)

    Woo Seok Yang

    2015-01-01

    Full Text Available The Cordyceps species has been a good source of compounds with anticancer and anti-inflammatory activities. Recently, we reported a novel compound (4-isopropyl-2,6-bis(1-phenylethylphenol, KTH-13 with anticancer activity isolated from Cordyceps bassiana and created several derivatives to increase its pharmacological activity. In this study, we tested one of the KTH-013 derivatives, 4-isopropyl-2,6-bis(1-phenylethylaniline 1 (KTH-13-AD1, with regard to anti-inflammatory activity under macrophage-mediated inflammatory conditions. KTH-13-AD1 clearly suppressed the production of nitric oxide (NO and reactive oxygen species (ROS in lipopolysaccharide (LPS and sodium nitroprusside- (SNP- treated macrophage-like cells (RAW264.7 cells. Similarly, this compound also reduced mRNA expression of inducible NO synthase (iNOS and tumor necrosis factor-α (TNF-α, as analyzed by RT-PCR and real-time PCR. Interestingly, KTH-13-AD1 strongly diminished NF-κB-mediated luciferase activities and nuclear translocation of NF-κB family proteins. In accordance, KTH-13-AD1 suppressed the upstream signaling pathway of NF-κB activation, including IκBα, IKKα/β, AKT, p85/PI3K, and Src in a time- and dose-dependent manner. The autophosphorylation of Src and NF-κB observed during the overexpression of Src was also suppressed by KTH-13-AD1. These results strongly suggest that KTH-13-AD1 has strong anti-inflammatory features mediated by suppression of the Src/NF-κB regulatory loop.

  6. El liberalsocialismo para Bobbio y para nosotros

    OpenAIRE

    Bovero, Michelangelo

    2006-01-01

    Partiendo de las ideas sobre el liberalsocialismo en el pensamiento de Norberto BOBBIO y hallando en el ámbito del constitucionalismo el terreno propicio, no ya para un compromiso, sino para una forma de reconciliación entre la tradición liberal y la socialista, el autor propone una caracterización del liberalsocialismo articulada en dos niveles: en un “liberalsocialismo mínimo” (o liberalsocialismo para la democracia), entendido como un “mínimo indispensable para la democracia”, y en un “lib...

  7. Inactivation of certain insect pathogens by ultraviolet radiation

    International Nuclear Information System (INIS)

    The UV-sensitivity of two baculoviruses (granulosis virus, nuclear polyhedrosis virus) and two entomopathogenic microorganisms (Bacillus thuringiensis, Beauveria bassiana) was determined by radiation tests. In the far UV (254 nm) the stability, measured at an inactivation rate of 99%, was in declining order: nuclear polyhedra >= conidia of B. bassiana > granula > spores of B. thuringiensis >= vegetative cells of B. thuringiensis. In the near UV (285-380 nm) the following order could be found: conidia of B. bassiana >= nuclear polyhedra > spores of B. thuringiensis >= granula > vegetative cells of B. thuringiensis. Far UV had a much higher germicidal effect for all pathogens tested than near UV. (orig.)

  8. Inactivation of certain insect pathogens by ultraviolet radiation

    Energy Technology Data Exchange (ETDEWEB)

    Krieg, A.; Groener, A.; Huber, J.; Zimmermann, G.

    1981-01-01

    The UV-sensitivity of two baculoviruses (granulosis virus, nuclear polyhedrosis virus) and two entomopathogenic microorganisms (Bacillus thuringiensis, Beauveria bassiana) was determined by radiation tests. In the far UV (254 nm) the stability, measured at an inactivation rate of 99%, was in declining order: nuclear polyhedra >= conidia of B. bassiana > granula > spores of B. thuringiensis >= vegetative cells of B. thuringiensis. In the near UV (285-380 nm) the following order could be found: conidia of B. bassiana >= nuclear polyhedra > spores of B. thuringiensis >= granula > vegetative cells of B. thuringiensis. Far UV had a much higher germicidal effect for all pathogens tested than near UV.

  9. Adult polyphagous coleopterans overwintering in cereal boundaries

    DEFF Research Database (Denmark)

    Riedel, W; Steenberg, T

    1998-01-01

    . Generally the mortality was low in autumn and mid-winter, not exceeding 5%. A more pronounced post-winter mortality of up to 30% was observed at the end of hibernation. Only a negligible number were infected by the entomopathogenic fungi Beauveria bassiana and Paecilomyces farinosus. Infection experiments...... with B, bassiana isolates from Sitona lineatus, Tachyporus hypnorum and three species of ground beetles showed a similarly low susceptibility of B. lampros and A. dorsale to B. bassiana and, although Tachyporus spp. showed a distinct mycophagy and a significantly higher mortality, this could...

  10. Sweet pepper seed responses to inoculation with microorganisms and coating with micronutrients, aminoacids and plant growth regulators Tratamento de sementes de pimentão com microrganismos, micronutrientes, aminoácidos e reguladores de crescimento

    Directory of Open Access Journals (Sweden)

    Kênia Almeida Diniz

    2009-06-01

    Full Text Available Small sized seeds, such as the horticultural species, have limited quantities of reserves that can be balanced by coating then with essential nutrients for their initial development. In addition, inoculation of the seeds with microorganisms may protect the plants against phytopathogens, thus enhancing their growth. The present work had the objective of evaluate the physiological quality and seedling development of sweet pepper seeds and seedlings coated with several kind of films. Seeds were first coated with polymers and then with antagonistic microorganisms (Trichoderma viride, Trichoderma polysporhum, Trichoderma stromaticum, Beauveria bassiana, Metarhizium anisopliae, mycorrhizas, aminoacids, micronutrients and plant growth regulators. Evaluation was performed for percentage of germination and for seedling emergence, speed of emergence index, number of plants, dry mass of the aerial and root parts and height of the seedlings. Inoculation with Trichoderma viride increased the percentage and rate of the seedlings emergence Inoculation with Trichoderma viride, Metarhizium anisopliae and mycorrhizas promote better seedling development; seed microbiolization with microorganisms Trichoderma viride, T. polysporhum, T. stromaticum, Beauveria bassiana, Metarhizium anisopliae. Mycorrhizas mixture negatively affected seeds and seedling quality. Seed covering with plant growht regulator, at a 5 mL kg-1 dose increased the roots dry matter.Em sementes pequenas, como as de espécies hortícolas, as limitadas quantidades de reservas podem ser equilibradas por meio do seu recobrimento com nutrientes essenciais para o seu desenvolvimento inicial. Além disso, a inoculação dessas sementes com microrganismos, além de proteger as plantas contra fitopatógenos, pode promover o seu crescimento. Assim, objetivo do presente trabalho foi avaliar o efeito de tipos de revestimentos na qualidade de sementes e mudas de pimentão (Capsicum annum. Para tanto, as

  11. Crescimento, esporulação e viabilidade de fungos entomopatogênicos em meios contendo diferentes concentrações do óleo de nim (Azadirachta indica

    Directory of Open Access Journals (Sweden)

    Marques Renata Paro

    2004-01-01

    Full Text Available A associação de extratos de origem vegetal com fungos entomopatogênicos pode aumentar a eficiência do controle biológico de pragas, reduzir custos e impactos ambientais. No presente trabalho, avaliou-se, através da concentração inibitória mínima, o efeito do óleo de nim (NIM-I-GO sobre o crescimento, esporulação e viabilidade de Metarhizium anisopliae, Beauveria bassiana e Paecilomyces farinosus. Utilizou-se o meio BDA, contendo diferentes concentrações de óleo de nim (C1: 5% de óleo de nim, e sucessivamente concentrações iguais a fraction one-half da concentração anterior, até C11: 0,0048% de óleo de nim. O óleo de nim reduziu o crescimento de colônias de B. bassiana e P. farinosus, que não diferiram significativamente do controle apenas na concentração C11, mas para M. anisopliae o mesmo efeito foi observado com 0,039% de óleo de nim (C8. A esporulação também foi significativamente reduzida pelo óleo de nim, exceto na concentração C11 para B. bassiana; contudo, não se verificou efeito do óleo na viabilidade de esporos dos fungos.

  12. Infection of the malaria mosquito, Anopheles gambiae, with two species of entomopathogenic fungi: effects of concentration, co-formulation, exposure time and persistence

    NARCIS (Netherlands)

    Mnyone, L.L.; Kirby, M.J.; Lwetoijera, D.W.; Mpingwa, M.W.; Knols, B.G.J.; Takken, W.; Russell, T.L.

    2009-01-01

    Background - Entomopathogenic fungi Metarhizium anisopliae and Beauveria bassiana isolates have been shown to infect and reduce the survival of mosquito vectors. Methods - Here four different bioassays were conducted to study the effect of conidia concentration, co-formulation, exposure time and per

  13. Formulating entompathogens for control of boring beetles in avocado orchards

    Science.gov (United States)

    A foam formulation of Beauveria bassiana was adapted to control boring beetles in avocado orchards. The two geographically independent avocado growing areas in the United States are threatened by emerging diseases vectored by boring beetles. In the California growing region, Fusarium dieback is vect...

  14. Entomopathogenic fungi associated with Ixodes ricinus ticks

    DEFF Research Database (Denmark)

    Kalsbeek, Vibeke; Frandsen, F.; Steenberg, Tove

    1995-01-01

    to the Hyphomycetes. Paecilomyces farinosus and Verticillium lecanii were the predominant species. Other species, found only on engorged females were: Beauveria bassiana, B. brongniartii, P. fumosoroseus and V. araneanrm. Eight out of 1833 ticks collected from the vegetation and three out of 269 engorged nymphs were...

  15. First Record of a Neozygites species (Zygomycetes:Entomophthorales) Infecting Springtails (Insecta:Collembola)

    DEFF Research Database (Denmark)

    Steenberg, T; Eilenberg, J; Bresciani, J.

    1996-01-01

    A fungal pathogen from the Entomophthorales (Zygomycetes) was discovered in populations of the lucerne flea Sminthurus viridis (Collembola) collected from grassland and leguminous crops in Denmark during July to October. The morphology of the fungus was studied in springtails, collected live, whi...... Conidiobolus coronatus. Verticillium lecanii and Beauveria bassiana (Deuteromycotina:Hyphomycetes) were also isolated from S. viridis....

  16. Habitat selection of a parasitoid mediated by volatiles informing on host and intraguild predator densities

    DEFF Research Database (Denmark)

    Cotes, Belén; Rännbäck, Linda Marie; Björkman, Maria;

    2015-01-01

    both a parasitoid and its host, parasitoids may reduce the risk of intraguild predation (IGP) by avoiding such patches. In this study, we examined whether the presence of the entomopathogenic fungi Metarhizium brunneum and Beauveria bassiana in soil habitats of a root herbivore, Delia radicum, affects...

  17. Combining larval parasitoids and an entomopathogenicfungus for biological control of Sitophilus granarius (Coleoptera: Curculionidae) in stored grain

    DEFF Research Database (Denmark)

    Hansen, Lise Stengaard; Steenberg, Tove

    2006-01-01

    or in combination with a surface treatment of the grain with the entomopathogenic fungus Beauveria bassiana (Bals.) Vuillemin against S. granarius in units containing 9 kg of wheat over a period of 26 weeks. In the untreated units, the pest population increased about 5000 times. The highest level of pest...

  18. Microbial metabolism Part 14 Isolation and bioactivity evaluation of microbial metabolites of resveratrol

    Science.gov (United States)

    The fungi, Beauveria bassiana (ATCC 13144) and Penicillium chrysogenium (ATCC 9480) transformed resveratrol (1) to resveratrol-3-O-sulfate (4). The former, in addition, gave 5-methoxyresveratrol-3-O-ß-glucoside (2) with the latter yielding 5-methoxyresveratrol-3-O-sulfate (3). The structures were es...

  19. Entomopathogenic fungi as biological control agents of diamondback moth (Lepidoptera: Plutellidae)and compatibility with chemical insecticides

    Science.gov (United States)

    The objectives were to evaluate the efficiency of entomopathogenic fungi against Plutella xylostella (L.) and the compatibility of the most virulent isolates with some of the insecticides registered for use on cabbage crops. Pathogenicity tests used isolates of Beauveria bassiana, Metarhizium rileyi...

  20. Susceptibility of entomopathgenic fungi to OMRI certified surfactants for biopesticide applications

    Science.gov (United States)

    Three entomopathgenic fungi, Beauveria bassiana, Metarhizium brunneum,and Isaria fumosorosea were evaluated for their compatibility with six surfactants. The surfactants are certified to comply with the U.S. National organic standards and are permitted to be used in organic production systems. The f...

  1. Entomopathogenic fungi isolates to control the borer of yerba mate (Hedypathes betulinus Kluger (Coleoptera; CerambycidaeIsolados de fungos entomopatogênicos visando ao controle da broca da erva-mate (Hedypathes betulinus Kluger (Coleoptera; Cerambycidae

    Directory of Open Access Journals (Sweden)

    André Luis Pereira Fanti

    2013-09-01

    ênico Beauveria bassiana (Bals. Vuill. e 18 isolados de Metarhizium anisopliae (Metsch. Sorok.. Inicialmente, avaliou-se a virulência dos isolados imergindo adultos da broca da erva-mate em suspensão fungica contendo 1×108 conídios mL-1, e posteriormente os que obtiveram maior mortalidade confirmada, foram selecionados para segunda etapa, na qual avaliou-se a virulência e crescimento vegetativo, produção de conídios em meio de cultura, arroz e em cadáveres de broca. Destacaram-se três isolados de B. bassiana (UNIOESTE 4, UNIOESTE 52 e UNIOESTE 64, e um de M. anisopliae (IBCB 352, atingindo mortalidade confirmada igual ou superior a 90% na primeira etapa, sendo estes selecionados para etapa posterior, juntamente com isolado padrão, CG 716. O isolado IBCB 352 de M. anisopliae, apesar de ser o único da espécie a ser selecionado para a segunda etapa, provocou a maior mortalidade confirmada e conidiogênese em cadáveres de broca, indicando uma elevada produção de inóculo, sendo este, o indicado para a campo.

  2. Aviso para las viudas.

    Directory of Open Access Journals (Sweden)

    Francisco de Sales

    2000-01-01

    Full Text Available San Pablo instruye todos los prelados en la persona de su Timoteo, diciendo: «Honra las viudas que son verdaderamente viudas». Para ser, pues, verdaderamente viuda, son necesarias estas cosas

  3. Leer para Sophia

    OpenAIRE

    Cassany, Daniel

    2009-01-01

    Leer para Sophia re??ne algunos de mis trabajos m??s recientes sobre la lectura, desde perspectivas complementarias, te??ricas y pr??cticas, destacando el tema de futuro de la lectura electr??nica o en l??nea. El t??tulo de este volumen tiene un doble sentido : primero apela al significado griego del vocablo sophia (sabidur??a), para iluminar uno de los prop??sitos esenciales de la lectura: aprender y comprender; luego quiere agradecer ...

  4. Tiempo para un cambio

    Science.gov (United States)

    Woltjer, L.

    1987-06-01

    En la reunion celebrada en diciembre dei ano pasado informe al Consejo de mi deseo de terminar mi contrato como Director General de la ESO una vez que fuera aprobado el proyecto dei VLT, que se espera sucedera hacia fines de este aAo. Cuando fue renovada mi designacion hace tres aAos, el Consejo conocia mi intencion de no completar los cinco aAos dei contrato debido a mi deseo de disponer de mas tiempo para otras actividades. Ahora, una vez terminada la fase preparatoria para el VLT, Y habiendose presentado el proyecto formalmente al Consejo el dia 31 de marzo, y esperando su muy probable aprobacion antes dei termino de este ano, me parece que el 10 de enero de 1988 presenta una excelente fecha para que se produzca un cambio en la administracion de la ESO.

  5. Instalaciones para hotel rural

    OpenAIRE

    Roig Riera, José Antonio

    2015-01-01

    El presente proyecto cuenta con la información necesaria para el desarrollo de las instalaciones de un hotel rural. Dichas instalaciones son; la eléctrica, la de agua caliente sanitaria, las telecomunicaciones y la climatización. En el apartado de la instalación eléctrica, se han calculado todos los cables necesarios para el correcto funcionamiento del hotel, partiendo de las potencias demandadas en cada punto de consumo. Se ha calculado la línea general de alimentación, acomet...

  6. Instrucciones para autores

    OpenAIRE

    Cramer, Thomas

    2012-01-01

    Instrucciones para autoresNota preliminar: ¡Es de suma importancia que antesde enviar cualquier documento a la Revista GeologíaColombiana, lea y cumpla cuidadosamente cada uno delos subíndices de las Normas para autores!A partir del Volumen 35 (2010) recibimos artículosprincipalmente a través del sistema digital enhttp://www.revistas.unal.edu.co/index.php/geocol/index.Si quiere enviar un artículo, por favor regístrese comoAutor en el menú INGRESAR (aquí pueden registrarsetambién evaluadores u...

  7. Tempo para aprender vs tempo para brincar

    OpenAIRE

    Correia, Ana Rita Coelho

    2016-01-01

    Relatório de Estágio apresentado à Escola Superior de Educação de Paula Frassinetti para a obtenção de grau de Mestre em Educação Pré-Escolar e Ensino do 1º Ciclo do Ensino Básico Esta investigação surge da reflexão sobre a urgência atual, generalizada por parte de muitos pais e encarregados de educação, bem como de algumas instituições, no sentido de escolarizar as crianças precocemente, com vista a que estas possam chegar ao ensino básico “mais preparadas”, esquecendo-se muitas ...

  8. Mortality risk from entomopathogenic fungi affects oviposition behavior in the parasitoid wasp Trybliographa rapae

    DEFF Research Database (Denmark)

    Rännbäck, Linda-Marie; Cotes, Belen; Anderson, Peter;

    2015-01-01

    brunneum isolate KVL 04-57 and Beauveria bassiana isolate KVL 03-90. Furthermore, T. rapae oviposition behavior was assessed in the presence of these entomopathogenic fungi either as infected hosts or as infective propagules in the environment. Both fungi were pathogenic to D. radicum larvae and T. rapae...... adults, but with variable virulence. When host patches were inoculated with M. brunneum conidia in a no-choice situation, more eggs were laid by T. rapae in hosts of those patches compared to control and B. bassiana treated patches. Females that later succumbed to mycosis from either fungusi laid...... larvae. This was less pronounced for B. bassiana. Based on our results we propose that T. rapae can perceive and react towards IGP risk posed by M. brunneum but not B. bassiana to the foraging female herself and her offspring. Thus, M. brunneum has the potential to be used for biological control against...

  9. Aprendizaje cooperativo para ELE

    OpenAIRE

    Cassany, Daniel

    2004-01-01

    Presentación sucinta para docentes de español como lengua extranjera del conjunto de propuestas metodológicas conocidas bajo la denominación aprendizaje cooperativo : origen, fundamentación, principales principios psicopedagógicos y algunas propuestas prácticas.

  10. Consejos para pacientes

    OpenAIRE

    M. Eulalia Lucio-Villegas Menéndez; Laura López González; M. Isabel Gutiérrez Pérez; Natalia Aresté Lluch; M. Luisa Morató Agustí; Santiago Pérez Cachafeiro

    2014-01-01

    En el cuidado de las heridas es tan importante saber qué hacer como saber qué no hacer. Lo primero es valorar la gravedad de la lesión y saber si es necesario acudir a un centro sanitario o no. Si la herida es simple, lo más recomendable es hacer una buena limpieza con suero o agua tras lavado de manos, realizando la desinfección de esta con el antiséptico más adecuado. Los antisépticos no deben usarse para la limpieza de las heridas (usar suero fisiológico o agua del grifo) ni para la cur...

  11. Educando para la ignorancia

    Directory of Open Access Journals (Sweden)

    Miguel Durán

    1996-06-01

    Full Text Available Más que describir una situación particular, los autores buscan con este escrito motivar al estudiante para que asuma posiciones críticas; así como dar un campanazo de alerta a los profesores, en relación a los conocimientos que transmiten a sus alumnos y la forma en que lo hacen; todo con el ánimo de generar verdaderos espacios de reflexión en la universidad.

  12. Arquitectura para el conocimiento

    OpenAIRE

    Moreno-Torres Sánchez, Rosario; Sánchez Relinque, Javier

    2011-01-01

    La Málaga del siglo XXI se prepara para hacer de la investigación y de la innovación su nuevo sello. Entrevista a tres de los artífices de la urbanización y la edificación de la ampliación de Teatinos, clave en el eje sociocultural, científico y empresarial que conforman ya la capital, UMA y Parque Tecnológico.

  13. INICIATIVAS PARA EL CAMBIO

    Directory of Open Access Journals (Sweden)

    César Alfonso Defrancisco-Larrañaga

    2013-07-01

    Full Text Available El planeta no atraviesa por su mejor momento en materia de conservación y renovación de sus recursos naturales. Claramente en los últimos tiempos los recursos hídricos han venido haciéndose cada vez menores; el hombre ha notado con preocupación y vivido en carne propia este fenómeno, que de no ser tratado a tiempo, podría atentar contra la existencia misma. Esto lo ha alentado a buscar métodos que sirvan como solución para contrarrestar éste mal y crear conciencia entre sus iguales, para así poder garantizar su permanencia y la de su entorno durante muchas generaciones. En países desarrollados del continente europeo y en algunos lugares de América del norte, el uso de sistemas de reutilización y tratamiento de aguas grises y aprovechamiento de las aguas lluvias se ha convertido en una constante y nos están dando las pautas para poder seguir el ejemplo y así de esta manera poder aportar a la causa ambiental y a su vez crear un ahorro en nuestros gastos mensuales de consumo.

  14. 4-Isopropyl-2,6-bis(1-phenylethyl)aniline 1, an Analogue of KTH-13 Isolated from Cordyceps bassiana, Inhibits the NF-κB-Mediated Inflammatory Response.

    Science.gov (United States)

    Yang, Woo Seok; Ratan, Zubair Ahmed; Kim, Gihyeon; Lee, Yunmi; Kim, Mi-Yeon; Kim, Jong-Hoon; Cho, Jae Youl

    2015-01-01

    The Cordyceps species has been a good source of compounds with anticancer and anti-inflammatory activities. Recently, we reported a novel compound (4-isopropyl-2,6-bis(1-phenylethyl)phenol, KTH-13) with anticancer activity isolated from Cordyceps bassiana and created several derivatives to increase its pharmacological activity. In this study, we tested one of the KTH-013 derivatives, 4-isopropyl-2,6-bis(1-phenylethyl)aniline 1 (KTH-13-AD1), with regard to anti-inflammatory activity under macrophage-mediated inflammatory conditions. KTH-13-AD1 clearly suppressed the production of nitric oxide (NO) and reactive oxygen species (ROS) in lipopolysaccharide (LPS) and sodium nitroprusside- (SNP-) treated macrophage-like cells (RAW264.7 cells). Similarly, this compound also reduced mRNA expression of inducible NO synthase (iNOS) and tumor necrosis factor-α (TNF-α), as analyzed by RT-PCR and real-time PCR. Interestingly, KTH-13-AD1 strongly diminished NF-κB-mediated luciferase activities and nuclear translocation of NF-κB family proteins. In accordance, KTH-13-AD1 suppressed the upstream signaling pathway of NF-κB activation, including IκBα, IKKα/β, AKT, p85/PI3K, and Src in a time- and dose-dependent manner. The autophosphorylation of Src and NF-κB observed during the overexpression of Src was also suppressed by KTH-13-AD1. These results strongly suggest that KTH-13-AD1 has strong anti-inflammatory features mediated by suppression of the Src/NF-κB regulatory loop. PMID:26819495

  15. Toxicity of natural insecticides on the larvae of wheat head armyworm, Dargida diffusa (Lepidoptera: Noctuidae).

    Science.gov (United States)

    Reddy, Gadi V P; Antwi, Frank B

    2016-03-01

    The wheat head armyworm, Dargida (previously Faronta) diffusa (Walker) (Lepidoptera: Noctuidae), is widely distributed in North American grasslands and is most common on the Great Plains, where it is often a serious pest of corn and cereal crops. Six commercially available botanical or microbial insecticides used against D. diffusa were tested in the laboratory: Entrust(®) WP (spinosad 80%), Mycotrol(®) ESO (Beauveria bassiana GHA), Aza-Direct(®) (azadirachtin), Met52(®) EC (Metarhizium brunneum F52), Xpectro(®) OD (Beauveria bassiana GHA+pyrethrins), and Xpulse(®) OD (Beauveria bassiana GHA+azadirachtin). Concentrations of 0.1, 0.5, 1.0 and 2.0 fold the lowest labelled rates of formulated products were tested for all products, while for Entrust WP additional concentrations of 0.001 and 0.01 fold the label rates were also assessed. Survival rates were determined from larval mortality at 1-9 days post treatment application. We found that among the tested chemicals, Entrust(®) (spinosad) was the most effective, causing 83-100% mortality (0-17% survival rate) at day 3 across all concentrations. The others, in order of efficacy from most to least, were Xpectro(®) (B. bassiana GHA+pyrethrins), Xpulse(®)OD (B. bassiana GHA+azadirachtin), Aza-Direct(®) (azadirachtin), Met52(®) EC (M. brunneum F52), and Mycotrol(®) ESO (B. bassiana GHA). These products and entomopathogenic fungi caused 70-100% mortality (0-30% survivability) from days 7 to 9. The tested products and entomopathogenic fungi can be used in management of D. diffusa. PMID:26855414

  16. Potential for entomopathogenic fungi to control Triatoma dimidiata (Hemiptera: Reduviidae, a vector of Chagas disease in Mexico

    Directory of Open Access Journals (Sweden)

    María Guadalupe Vázquez-Martínez

    2014-12-01

    Full Text Available Introduction The use of entomopathogenic fungi to control disease vectors has become relevant because traditional chemical control methods have caused damage to the environment and led to the development of resistance among vectors. Thus, this study assessed the pathogenicity of entomopathogenic fungi in Triatoma dimidiata. Methods Preparations of 108 conidia/ml of Gliocladium virens, Talaromyces flavus, Beauveria bassiana and Metarhizium anisopliae were applied topically on T. dimidiata nymphs and adults. Controls were treated with the 0.0001% Tween-80 vehicle. Mortality was evaluated and recorded daily for 30 days. The concentration required to kill 50% of T. dimidiata (LC50 was then calculated for the most pathogenic isolate. Results Pathogenicity in adults was similar among B. bassiana, G. virens and T. flavus (p>0.05 and differed from that in triatomine nymphs (p=0.009. The most entomopathogenic strains in adult triatomines were B. bassiana and G. virens, which both caused 100% mortality. In nymphs, the most entomopathogenic strain was B. bassiana, followed by G. virens. The native strain with the highest pathogenicity was G. virens, for which the LC50 for T. dimidiata nymphs was 1.98 x108 conidia/ml at 13 days after inoculation. Conclusions Beauveria bassiana and G. virens showed entomopathogenic potential in T. dimidiata nymphs and adults. However, the native G. virens strain presents a higher probability of success in the field, and G. virens should thus be considered a potential candidate for the biological control of triatomine Chagas disease vectors.

  17. 福建林业白僵菌产业发展对策与建议%Primarily Discussion on the Status and Development of Beauveria in Fujian Province

    Institute of Scientific and Technical Information of China (English)

    陈昌谋

    2011-01-01

    Beauveria bassiana(Bals)Vuill has been the main biocontrol pestcide in Fujian Province during the past 20 years. There are 13 factories in Fujian,but the factories are obstracted development due to small --scale,low--benefit,old equipment,high consumption,single--produce and unsteady control effects. There are the following important ways for these factories to get out of current situation:to break the system limitation and introduce market mechanism;to strengthen the cultivation of talents,strengthen the research and innovation on strains, dosage forms, screening in application and the methods; to research other biological agents,fire fighting spirit,edible fungus,etc based on bacteria factory; and to set up the trademark and brand awareness and expand the products to markets at home and abroad by the modern marketing means .%指出了福建省白僵菌产业存在技术人员缺乏、生产规模小、效益低、设备陈旧、生产成本高及菌剂单一、防治效果不稳定等问题,从加强人才培养,以菌厂为基地,研发其他生物制剂、灭火灵、食用菌等多种经营,树立商标和品牌意识,应用现代营销手段把产品推向国内外市场等方面探讨了福建白僵菌产业化发展的对策。

  18. para mejorar el %R

    Directory of Open Access Journals (Sweden)

    Alfredo Díaz Mata

    2001-01-01

    Full Text Available En este trabajo se revisan dos indicadores técnicos bursátiles que están estrechamente relacionados, el oscilador estocástico %K de Lane y el oscilador %R de Williams; asimismo, se evalúa el uso de dos mecanismos asociados con el primero y que no suelen utilizarse con el %R: el proceso de frenado (slowing y el uso de un promedio móvil del propio oscilador estocástico como disparadores de señales de compra y de venta. Realizando simulaciones con 27 acciones y el Índice de Precios y Cotizaciones de la Bolsa Mexicana de Valores se verifica la hipótesis principal y se comprueba que, efectivamente, en muchos casos, el uso del proceso de frenado con el oscilador %R de Williams permite tomar mejores decisiones. Por otro lado, se concluye que de los dos disparadores de señales el que mejor funciona, tanto para %K como para %R es el uso de cotas inferior y superior. Este artículo surgió en el curso de una investigación sobre el análisis técnico bursátil de acciones que se puede realizar en su totalidad por computadora y que, esencialmente, excluye el análisis gráfico. Uno de los propósitos del estudio es determinar si es posible, y bajo qué mecanismos, establecer un sistema de análisis bursátil que le permita al inversionista tomar decisiones de inversión en acciones en la Bolsa Mexicana de Valores sin tener que emplear la considerable cantidad de tiempo que es necesario para el análisis gráfico tradicional. Durante este estudio se realizó un análisis preliminar de unos 120 indicadores técnicos para, por un lado, conocerlos con cierto detalle y, por otra parte, aislar aquellos que prometían ser de utilidad para el objetivo de la investigación. Fue en este proceso cuando se detectó que existen dos indicadores que están relacionados y que permiten un análisis por separado que tiene, cuando menos, dos ventajas. En primer lugar está el placer que da a quien gusta de estos temas encontrarse con indicadores que, de acuerdo

  19. Inmunopotenciadores para la acuicultura

    Directory of Open Access Journals (Sweden)

    Rocmira Pérez

    2014-04-01

    Full Text Available La acuicultura es una de las actividades económicas de mayor crecimiento para la producción de alimentos. Uno de sus principales retos es la obtención de grandes volúmenes de producción con la mayor calidad posible. Esto conlleva a una reducción de la aplicación de antibióticos y productos quimioterapéuticos. Una de las estrategias más prometedoras es la aplicación de inmunopotenciadores, principalmente en los cultivos intensivos. El objetivo de este trabajo fue revisar los principales inmunopotenciadores, así como las tendencias y retos de su uso mundial. Se resumen las particularidades moleculares y funcionales de los mismos y se hace énfasis en los más estudiados: levamisol, ß-glucanos, lipopolisacárido, vitamina C, extractos de plantas y hormonas. Todos estos compuestos de naturaleza heterogénea inciden mayoritariamente en los componentes de la inmunidad innata de los peces, fortaleciendo y potenciando la resistencia a enfermedades; adicionalmente algunos de ellos tienen funciones antiestrés y favorecen su crecimiento. Se concluye que los inmunopotenciadores constituyen una estrategia viable para reducir las pérdidas por problemas sanitarios en el sector de la acuicultura; pero aún quedan por solucionar aspectos como la vía de administración y la etapa de inmunización adecuada para cada especie y tipo de cultivo.

  20. New solid-state fermentation chamber for bulk production of aerial conidia of fungal biocontrol agents on rice.

    Science.gov (United States)

    Ye, S D; Ying, S H; Chen, C; Feng, M G

    2006-06-01

    A novel solid-state fermentation apparatus, namely an upright multi-tray conidiation chamber, was developed to facilitate the production of aerial conidia of fungal biocontrol agents, such as Beauveria bassiana. The chamber with 25 bottom-meshed metal trays had a capacity of > or =50 kg rice with each tray holding > or =2 kg. In repeated trials, a mean yield of 2.4 (1.8-2.7) x 10(12) conidia kg(-1) rice was harvested from the 7-day cultures of B. bassiana in a fully loaded chamber. The new apparatus has a high potential for bulk production of fungal conidia. PMID:16786244

  1. Disease symptoms occurring in caterpillars of Malacosoma neustria L. (Lepidoptera infected by some fungi

    Directory of Open Access Journals (Sweden)

    Zofia Machowicz-Stefaniak

    2014-11-01

    Full Text Available The infection of M. neustria caterpillars by Beauveria bassiana (Bals. Vuill., Metarrhizium anisopliae (Metsch. Sorok., Paecilomyces farinosus (Dicks. ex Fr. Brown et Smith, Verticillium lecanii (Zimm. Viegas causes the decrease of their viability, paralysis and mummification of the body. Only in the case of B. bassiana the character distinguishing infection by this fungus are black spots on the surface of the cuticle. The easy development and aboundant spore formation of the above-mentioned hyphomycetous fungi on M. neustria facilitate the determination of the causes of the disease.

  2. Entomopathogenic fungi for control of arthropod pests in egg production facilities

    DEFF Research Database (Denmark)

    Steenberg, Tove; Kilpinen, Ole

    Beauveria bassiana and other species of entomopathogenic fungi are potential candidates for microbial control of major pests in egg layers, e.g. the poultry red mite (Dermanyssus gallinae), the housefly (Musca domestica) and the darkling beetle (Alphitobius diaperinus). We have selected an isolate...... of B. bassiana with high efficacy against all target pests in laboratory assays, and will review the existing information on the natural occurrence of these fungi in farms with confined animals and discuss the possibilities and constraints for exploitation of entomopathogenic fungi as control agents...

  3. Integrated pest management and entomopathogenic fungal biotechnology in the Latin Americas: II key research and development prerequisites

    International Nuclear Information System (INIS)

    In the first part of this review article (Valencia and Khachatourians, 1998) we presented the special opportunity that entomopathogenic fungi (EPF) offer for integrated pest management (IPM) in the Latin Americas. As expected, along with the opportunities, there are challenges for the use of EPF. First that there are only two fungi, Beauveria bassiana and Metarhizium anisopliae, for which some prerequisite knowledge of basic and applied mycology for industrial research and development (R and D) are in place. Because of precedent setting leadership in the development of certain EPF, e.g., B. bassiana in IPM, Latin America stands to contribute to and gain from future

  4. Transcriptional control of fungal cell cycle and cellular events by Fkh2, a forkhead transcription factor in an insect pathogen

    OpenAIRE

    Wang, Juan-juan; Qiu, Lei; Cai, Qing; Ying, Sheng-Hua; Feng, Ming-Guang

    2015-01-01

    Transcriptional control of the cell cycle by forkhead (Fkh) transcription factors is likely associated with fungal adaptation to host and environment. Here we show that Fkh2, an ortholog of yeast Fkh1/2, orchestrates cell cycle and many cellular events of Beauveria bassiana, a filamentous fungal insect pathogen. Deletion of Fkh2 in B. bassiana resulted in dramatic down-regulation of the cyclin-B gene cluster and hence altered cell cycle (longer G2/M and S, but shorter G0/G1, phases) in unicel...

  5. Instrucciones para los autores

    OpenAIRE

    Ana Silvia Bermúdez

    2011-01-01

    La Revista Colombiana de Química es una publicación científica, arbitrada del Departamento de Química, Facultad de Ciencias, Universidad Nacional de Colombia que acepta contribuciones provenientes de investigación en las diversas áreas de la química. El contenido de los artículos debe ser original, inédito y no debe haber sido enviado, total o parcialmente, para publicación a otra revista. La redacción asume el derecho de reproducción de los trabajos aceptados. Su publicación en otro medio re...

  6. Comunicar para la salud

    OpenAIRE

    Lic. Eva Bocco

    1998-01-01

    Con este trabajo quiero plantearles una inquietud en torno al rol que nos cabe a los comunicadores en el área de la salud desde la perspectiva de la comunicación para la salud. ¿Se puede pensar la salud pública desvinculada de la comunicación? ¿La comunicación es posible sin la existencia de salud en la comunidad? ¿Qué puede hacer un comunicador en el ministerio de salud? ¿Puede un estado desarrollar políticas de salud sin la presencia de la comunicación?

  7. No benefit in diversity? The effect of genetic variation on survival and disease resistance in a polygynous social insect

    DEFF Research Database (Denmark)

    Schmidt, Anna Mosegaard; Linksvayer, Timothy Arnold; Boomsma, Jacobus Jan;

    2011-01-01

    within-worker variation).3. We found significant differences in worker survival among the three inbred lineages, with exposure to conidiospores of the fungal pathogen Beauveria bassiana causing significant mortality to the workers independently of their diversity type. Increased diversity did not improve...... the resistance to Beauveria.4. Enhanced heterozygosity colonies had worker survival rates similar to the most resistant inbred lineage, whereas colonies with mixed workers from the three inbred lineages had lower worker and larval survival. Workers did not show any infection-avoidance behaviour.5. Average larval...

  8. "Colaborar para Competir"

    Directory of Open Access Journals (Sweden)

    Martha Liliana Hijuelos-Cárdenas

    2016-08-01

    Full Text Available De acuerdo con Michael Porter, los Clusters se definen como “Una agrupación de empresas e instituciones relacionadas entre sí, pertenecientes a un mismo sector o segmento de mercado, que se encuentran próximas geográficamente y que colaboran para ser más competitivas”. Los Clusters son en última instancia una herramienta de competitividad basada en la cooperación de sus miembros, y es una de las estrategias recientes, en el país, para incrementar la productividad y competitividad y las apuestas productivas desde el nivel local. Y es precisamente esta cercanía geográfica de las empresas de un mismo sector, lo que a la vez que genera la competencia entre ellas, también les facilita el acceso a insumos y a conocimientos específicos y en última instancia les permite promover la innovación.

  9. Requerimientos para aplicaciones web

    OpenAIRE

    Oliveros, Alejandro; Wehbe, Ricardo; Rojo, Silvana del Valle; Rousselot, Julián

    2011-01-01

    El objetivo del presente proyecto es la elaboración de un conjunto de políticas, estándares, procesos, metodologías, técnicas, herramientas y métricas para aplicar en el desarrollo de aplicaciones Web, específicamente en la obtención y representación de los requerimientos que debe satisfacer el sistema o aplicación Web. Estos distintos elementos se desarrollarán en forma gradual y eventualmente se integrarán en un sitio Web que centralice toda la información. Hay varias líneas de investigació...

  10. Contratados peninsulares para Cuba

    Directory of Open Access Journals (Sweden)

    Iglesias García, Fe

    1994-12-01

    Full Text Available Not available.

    Ante las presiones británicas, la metrópoli española y los ideólogos de los hacendados cubanos tuvieron que dar, desde la tercera década del siglo XIX, una respuesta a la previsible escasez de mano de obra esclava. El artículo estudia los sistemas de contrata implantados en la isla para abastecer de mano de obra blanca y barata a Cuba, deteniéndose particularmente la autora en el estudio de las condiciones de semiesclavitud que se escondían en las mencionadas contratas y en el perfil de los inmigrantes (profesiones, sexo, edad, procedencia....

  11. Bases para proyectiles dirigidos

    Directory of Open Access Journals (Sweden)

    Editorial, Equipo

    1959-03-01

    Full Text Available Aunque actualmente no se ha llegado a una línea general de métodos o sistemas que gobiernen un tipo característico de rampa y servicios auxiliares necesarios para el lanzamiento al espacio de proyectiles dirigidos a grandes alturas y distancias, las experiencias obtenidas en diferentes ensayos, utilizando distintos tipos de proyectiles y trayectorias balísticas, han sentado toda una serie de procedimientos, datos y conclusiones de gran valor balístico que, aun teniendo en cuenta la continua evolución del proyectil, sus formas, combustibles y alcances, se conocen ya, con bastante aproximación, las condiciones mínimas que ha de reunir una base dedicada a este tipo de lanzamientos.

  12. 白僵菌生产中发酵罐培养技术研究%Technology Research of Liquid Fermentation Tank Culture of Beauveriabassiana

    Institute of Scientific and Technical Information of China (English)

    戈媛媛; 赵东容; 沈伟清; 张建华

    2015-01-01

    In order to master the technology of fermentation culture of Beauveria bassiana ,and put the culture of ex‐panding Beauveria bassiana into industrial production ,we used Beauveria bassiana strain of Yiling district ,Yichang as the material and fermentation technical parameters such as the appropriate liquid fermentation medium and the growth mor‐phology of strain and pH values of growth environment were studied by liquid fermentation tank culture which could help identify the time of transferring liquid from seed tank to fermentation tank .The results showed that Carbon ,Nitrogen and Phosphorus were three kinds of necessary nutrient elements in the liquid fermentation .Besides ,these three essential factors for the growth of Beauveria bassiana had different proportion ,the addition of a small amount of microelement in the liquid fermentation medium at the early stage could increase growth rate and the number of Beauveria bassiana evi‐dently;Beauveria bassiana has the cyclic growth history that is mutual transformation between saprophyte and mycelium in its multistage continuous liquid fermentation ,and the comparative analysis of spore form and the numbers showed that the fastest growth appeared in the pH value between 4 and 5 which the growth environment of Beauveria bassiana owned by self‐regulation and artificial adjustment ,although we had given condition that the medium was a pH of 6 initially;Considering the growth morphology and the number and the activity of Beauveria bassiana and the workshop operation schedule ,we made the following arrangements ,and the first process was producing mycelium in the seed tank for 42~45 hours ,and the second one was producing saprophyte which was the strain of solid fermentation production in the fermen‐tation tank for 21 to 26 hours .%为掌握白僵菌发酵罐培养技术,白僵菌可工业化生产,以宜昌夷陵区白僵菌菌种为材料,对白僵菌液体发酵罐发酵培养的培养基、菌种生

  13. Effects of biorational pesticides on four coccinellid species (Coleoptera: Coccinellidae) having potential as biological control agents in interiorscapes.

    Science.gov (United States)

    Smith, S F; Krischik, V A

    2000-06-01

    The direct toxicity of insecticidal soap, horticultural oil, Azatin, an extract from the Neem tree containing azadiractin, and BotainiGard, a commercial formulation of the entomopathogenic fungus Beauveria bassiana, was assessed on adults of four species of coccinellids--Hippodamia convergens (Guérin-Ménéville), Coleomegilla maculata (DeGeer), Harmonia axyridis Pallas, and Cryptolaemus montrouzieri Mulsant. All biorationals caused less mortality than a conventional pesticide, carbaryl (Sevin). Horticultural oil (Sunspray ultrafine oil) consistently had no effect on beetle survivorship. Insecticidal soap (M-Pede) significantly reduced survival in all replicates for C. maculata and in at least one of the three replicates for the other three coccinellid species. Beauveria bassiana (BotaniGard) significantly reduced survival of C. montrouzieri at 72 h after spray in all three replicates. Azatin reduced survivorship in only one species, C. maculata, in only one of the three replicates. PMID:10902323

  14. Para Para舞不停

    Institute of Scientific and Technical Information of China (English)

    周鹏程

    2002-01-01

    @@ 提问:目前在全亚洲最热门的舞蹈是什么? 回答:HIP HOP. 错!错!错!是Para Para! 君不见,从日本到韩国,从马来西亚到新加坡,从香港到台湾,无论是公园广场,还是迪厅PARTY,到处都涌动着这股来势汹汹的Para Para潮.而且,偷偷告诉你,最近这股Para Para风暴已经悄悄在内地登陆了!各位时尚MMGG们,准备好了吗?让我们一起投身Para Para大潮!

  15. Desarrollo de una metodología de crianza en laboratorio del gusano cogollero del maíz Spodoptera frugiperda (Smith (Lepidoptera: Noctuidae como posible hospedante de insectos biocontroladores de interés agrícola

    Directory of Open Access Journals (Sweden)

    Yerlin Chacón Castro

    2009-11-01

    Full Text Available Con el fin de desarrollar una metodología de crianza en laboratorio que permitala producción masiva de organismos biocontroladores de Spodopterafrugiperda, se colectó ejemplares en estado larval en cultivares de maíz de diferentes localidades de Costa Rica. Dichas larvas fueron transportadas hasta el Laboratorio de Biocontroladores del CIB-ITCR, Cartago.Al inicio del ensayo, las larvas fueron alimentadas con hojas tiernas demaíz por espacio de dos meses. Luego se evaluó la dieta artificial BIO-MIX H-89,modificada para el desarrollo de huevo hasta pupa y durante la fase de adulto,se utilizó una solución de miel de abeja al 10% (v/v como fuente de alimento.Se aplicó un ANOVA para medir las diferencias entre cada etapa del ciclo devida con ambas dietas y se encontró que al implementar la dieta artificial, se redujo elciclo de vida de esta especie a 45,10±1,20 días, y se prolongó el periodo larval a 26,80±1,41 días. Asimismo, se identificó el hongo entomopatógeno Beauveria bassiana como controlador biológico de esta especie.

  16. Bacillus thuringiensis: una aplicación de la ciencia

    Directory of Open Access Journals (Sweden)

    Ninfa María Rosas García

    2014-12-01

    Full Text Available Decía Louis Pasteur “Il n’y a pas des sciences appliquées…mais il y a des applications de la science” que significa “No hay ciencias aplicadas…sino aplicaciones de la ciencia”. Desde el siglo antepasado el uso de herramientas biológicas para el control de plagas de insectos, empezó a comprenderse como una posibilidad de contrarrestar el efecto nocivo de las plagas en los cultivos, sin embargo fueron necesarios casi dos siglos para que sus ventajas así como su eficiencia y eficacia fueran realmente aprovechadas. Recordando un poco de historia en 1835 Agostino Bassi, científico italiano, descubrió una enfermedad infecciosa en Bombix mori, mejor conocido como el gusano de seda, causada por la muscardina blanca, Beauveria bassiana, lo que le valió una inmensa notoriedad. Varias décadas después Pasteur y Koch redescubrieron sus principios y Eli Metchnikoff en 1878 puso en práctica el uso de la muscardina verde, Metarhizium anisopliae, contra larvas del escarabajo Anisoplia austriaca.

  17. Pequenos para idade gestacional: fator de risco para mortalidade neonatal

    OpenAIRE

    Almeida Márcia Furquim de; Jorge Maria Helena Prado de Mello

    1998-01-01

    OBJETIVO: Estudar as variáveis contempladas na Declaração de Nascimento (DN) como possíveis fatores de risco para nascimentos pequenos para a idade gestacional (PIG) e o retardo de crescimento intra-uterino como fator de risco para a mortalidade neonatal. MATERIAL E MÉTODO: As variáveis existentes na DN foram obtidas diretamente de prontuários hospitalares. Os dados referem-se a uma coorte de nascimentos obtida por meio da vinculação das declarações de nascimento e óbito, correspondendo a 2.2...

  18. Cotton in Benin: governance and pest management

    OpenAIRE

    Togbe, C.E.

    2013-01-01

    Key words: cotton, synthetic pesticides, neem oil (Azadirachta indica), Beauveria bassiana, Bacillus thuringiensis, field experiment, farmers’ participation   Pests are one of the main factors limiting cotton production worldwide. Most of the pest control strategies in cotton production rely heavily on the application of synthetic pesticides. The recurrent use of synthetic pesticides has large consequences for the environment (air, water, fauna, and flora) and human health. In cott...

  19. Biochemiluminescence of certain fungi

    OpenAIRE

    Janusz Sławiński; Danuta Sławińska; Irena Majchrowicz

    2014-01-01

    Twelve species of fungi growing on the Sabouraud medium in darkness and illumination in an incubator, were tested to find out their ability to emit the ultra-weak biochemiluminescence. Using a sensitive photon-counling device, it was possible to measure biochemiluminescence intensity during ten days of cultures growth. Boletus edulis, Pestalotia funerea and Microsporum gypseum displayed biochemiluminescence, while Aspergillus nidulans, A. quadrilineatus, Beauveria bassiana, Macrophoma candoll...

  20. Entomopathogenic Fungi in Flies Associated with Pastured Cattle in Denmark

    DEFF Research Database (Denmark)

    Steenberg, Tove; Jespersen, Jørgen B.; Jensen, Karl-Martin Vagn;

    2001-01-01

    Cattle flies, including Musca autumnalis, Haematobia irritans, and Hydrotaea irritans, are pests of pastured cattle. A 2-year study of the natural occurrence of entomopathogenic fungi in adult cattle flies and other flies associated with pastures showed that the four species included in the Entom...... for a revision of the two species. Low prevalences were recorded of another entomophthoralean, Furia americana, and of the hyphomycetes Beauveria bassiana and Verticillium lecanii....

  1. S-(−)-10,11-Dihydroxyfarnesoic Acid Methyl Ester Inhibits Melanin Synthesis in Murine Melanocyte Cells

    OpenAIRE

    Seung-Hwa Baek; Jun-Won Ahn; Sung-Hee Nam; Cheol-Sik Yoon; Jae-Cheon Shin; Sang-Han Lee

    2014-01-01

    The development of antimelanogenic agents is important for the prevention of serious aesthetic problems such as melasmas, freckles, age spots, and chloasmas. In the course of screening for melanin synthesis inhibitors, we found that the culture broth from an insect morphopathogenic fungus, Beauveria bassiana CS1029, exhibits potent antimelanogenic activity. We isolated and purified an active metabolite and identified it as S-(−)-10,11-dihydroxyfarnesoic acid methyl ester (dhFAME), an insect j...

  2. Leer para Creer

    Directory of Open Access Journals (Sweden)

    Roberto Polanco-Carrasco

    2011-12-01

    Full Text Available Más allá de los prejuicios que se puedan alimentar en las aulas por -en ocasiones- brillantes exponentes de la clínica psicológica, resultaría difícil creer que exista hoy en día un profesional que se atreva a sostener que el ser humano es sólo mente, o argumentar que lo corporal o cerebral es secundario e incluso irrelevante en los pacientes con los que habitualmente se deberá tratar. De hacerlo simplemente sería un acto de ignorancia, sobretodo en un momento en que disponemos de evidencias diarias desde las neurociencias que dan para sostener la unidad mente-cuerpo como nunca antes en la historia del estudio de “lo psicológico”. La convicción de que los estudiantes deberán ser nutridos con las nociones básicas de los modernos estudios del cerebro debería reflejarse en la bibliografía de diversas asignaturas y estar presente en el espíritu central del quehacer académico como una manera de prevenir esta viciosa tendencia a ningunear aquello que se desconoce y que finalmente cegará a la hora de probar nuevas formas de ver “el síntoma”.

  3. para promover la salud

    Directory of Open Access Journals (Sweden)

    Ma. del Consuelo Chapela

    2002-01-01

    Full Text Available Hablar de educación a distancia sustentada en el desarrollo de la cibernética obliga a reflexionar sobre sus definiciones, objetivos y medios, especialmente si pensamos en las necesidades de la fracción de población que no tiene acceso a las posibilidades que ofrece el desarrollo tecnológico. En este trabajo se presenta e ilustra con un ejemplo práctico, una perspectiva sobre las posibilidades de la educación a distancia desde las necesidades de la promoción de la salud entre grupos de población marginada en el mundo virtual. Se propone la creación de bibliotecas itinerantes regionales que pongan a disposición de la población con acceso limitado a la información, la tecnología comunicativa necesaria para apoyar los trabajos de promoción de la salud.

  4. Marcadores para la Realidad Aumentada para fines educativos

    Directory of Open Access Journals (Sweden)

    María Reina Zarate Nava

    2013-12-01

    Full Text Available La Realidad Aumentada (AR es considerada por algunos expertos como la tecnología en crecimiento para el 2014, en diferentes sectores: ingeniería, educación, juegos y negocios, siendo este último el más usado en él 2013. Sin embargo en el sector educativo la AR se presenta en algunos proyectos que se han desarrollado para diferentes áreas de las ciencias, como Química, Física y Matemáticas, lo anterior con el objetivo de facilitar el proceso de Enseñanza-Aprendizaje. En el presente artículo se presenta el modelo de patrones utilizado para un Proyecto AR enfocado en Química, el cual presenta un conjunto de patrones que fueron valorados para facilitar el rastreo de los objetos y a su vez el entendimiento de algunos materiales de los laboratorios de Química I.

  5. Plataforma VMware para academia virtual

    OpenAIRE

    Martínez Sánchez, José María

    2014-01-01

    Este PFC surge para dar respuesta a los problemas que se presentan en una unidad educativa a la hora de diseñar y crear una nueva infraestructura de red, servidores y equipos de trabajo que se presenta en el diseño y puesta en marcha de una nueva entidad educativa. Se proyecta la creación y configuración de una infraestructura virtual que aborde todos estos problemas, ofreciendo la solución mediante un único nodo virtual. Para ello, utilizamos sw de VMware para la creación de dicho nodo virtu...

  6. Una agenda para educadores

    Directory of Open Access Journals (Sweden)

    Robert O. McClintock

    2007-01-01

    Full Text Available Las instituciones educativas constituyen los principales factores en la construcción social de un nuevo sistema educativo. Las escuelas y los educadores controlan y producen propiedad intelectual de una extraordinaria profundidad y amplitud. Los cambios producidos por las tecnologías digitales están consiguiendo que el acceso y la producción de conocimiento sea más accesible y universal. Lo que es, a la vez, un gran logro de apertura curricular, pero también un grave peligro de perder la perspectiva eminentemente “educativa” de la transmisión del conocimiento en la escuela. Los nuevos recursos permiten a los educadores romper el ciclo de reproducción en los logros educativos, acercarse al medio y a la familia y transformar la escuela. Los educadores y las escuelas deben asumir el reto de constituir comunidades de aprendizaje. Así como de atreverse a producir y difundir ese conocimiento con soporte digital. Lo contrario sería una terrible abdicación. El artículo traza un recorrido histórico sobre esta función de la escuela y su adaptación a los medios y recursos de cada época. Tras lo cual, aboga por asumir el desafío actual de las TICs, para renovar el vínculo progresista con la posteridad y construir un mejor futuro. Los nuevos recursos permiten a los educadores romper el ciclo de reproducción en los logros educativos, acercarse al medio y a la familia y transformar la escuela.

  7. Proyecto de red para ONG

    OpenAIRE

    Ruiz López, David

    2013-01-01

    Proyecto de una red de comunicaciones para una ONG en la Comunidad de Madrid. Projecte d'una xarxa de comunicacions per a una ONG a la Comunidad de Madrid. Bachelor thesis for the Telecommunication Technologies program on Telematics.

  8. Evaluación del riesgo ambiental de carbofurano en bioensayos con organismos no blanco

    Directory of Open Access Journals (Sweden)

    José Alberto Iannacone

    2011-06-01

    Full Text Available El carbofurano es uno de los plaguicidas más empleado en la agricultura peruana. El objetivo de este trabajo fue evaluar el riesgo ambiental del carbofurano en bioensayos de toxicidad, sobre once especies no destinatarias. La siguiente secuencia relativa de mayor a menor cocientes de riesgo (CR del carbofurano para los 19 puntos finales de efecto en once especies fue encontrada: Daphnia magna (mortalidad > Lemna minor (inhibición de formación de las hojas > Paracheirodon innesi (nado extraño = P. innesi (incremento del movimiento opercular > Chironomus calligraphus (mortalidad > L. minor (necrosis > P. innesi (mortalidad > L. minor (clorosis > Tetrapygus niger (inhibición de la fecundación > Coturnix japonica (mortalidad > Oncorhynchus mykiss (incremento de coloración > Ceraeochrysa cincta (no eclosión de huevos > O. mykiss (mortalidad con oxígeno > O. mykiss (mortalidad sin oxígeno > Trichogramma pretiosum (mortalidad > C. cincta (mortalidad > T. pretiosum (no emergencia de adultos > Trichogramma pintoi (mortalidad > Beauveria bassiana (inhibición del crecimiento. Los cocientes de riesgo (CR indicaron en todos los casos un alto riesgo del carbofurano principalmente en el ambiente acuático en comparación con el terrestre.Carbofuran is one of the most employed pesticides in Peruvian agriculture. This research aimed to evaluate the risk assessment in bioassays of carbofuran on eleven non-target species. The following relative sequence in decreasing ecotoxicity order in terms of RQ (Risk quotient to carbofuran to 19 end points on 11 species was found: Daphnia magna (mortality > Lemna minor (inhibition of new fronds > Paracheirodon innesi (strange swim = P. innesi (increase of opercula movement > Chironomus calligraphus (mortality > L. minor (necrosis > P. innesi (mortality > L. minor (chlorosis > Tetrapygus niger (inhibition of fertilization> Coturnix japonica (mortality > Oncorhynchus mykiss (increase of coloration > Ceraeochrysa

  9. Planar Para Algebras, Reflection Positivity

    CERN Document Server

    Jaffe, Arthur

    2016-01-01

    We define the notion of a planar para algebra, which arises naturally from combining planar algebras with the idea of $\\Z_{N}$ para symmetry in physics. A subfactor planar para algebra is a Hilbert space representation of planar tangles with parafermionic defects, that are invariant under isotopy. For each $\\Z_{N}$, we construct a family of subfactor planar para algebras which play the role of Temperley-Lieb-Jones planar algebras. The first example in this family is the parafermion planar para algebra. Based on this example, we introduce parafermion Pauli matrices, quaternion relations, and braided relations for parafermion algebras which one can use in the study of quantum information. Two different reflections play an important role in the theory of planar para algebras. One is the adjoint operator; the other is the modular conjugation in Tomita-Takesaki theory. We use the latter one to define the double algebra and to introduce reflection positivity. We give a new and geometric proof of reflection positivi...

  10. Influence of culture media in viability test of conidia of entomopathogenic fungi Influência de meios de cultura em teste de viabilidade de fungos entomopatogênicos

    Directory of Open Access Journals (Sweden)

    Edimara Aparecida Francisco

    2006-08-01

    Full Text Available This work aimed at investigatimg whether the culture medium used in viability tests affects the conidial germination of Lecanicillium lecanii, Beauveria bassiana and Paecilomyces fumosoroseus isolates. The tests were performed on microscope slides containing one of the culture media: agar-water (AW, minimal medium (MM, potato-dextrose agar (PDA, potato-dextrose-1% yeast extract agar (PDAY, Sabouraud-dextrose-yeast extract agar (SDAY, and complete medium (CM. Three areas per slide were delimited and 0.05ml of a 5.5 x 105 conidia ml-1 suspension was applied to each area. One bioassay was performed for each isolate. Germination was determined after 15 hours of incubation at 26±0.5°C. The culture media influenced the germination of the species studied, verifying within and inter specific variations. CM and PDA provided the highest germination of L. lecanii isolates and the lowest was obtained on SDAY and AW. The germination of B. bassiana isolates was favoured by CM, PDA and PDAY media, a fact not observed in AW and MM. P. fumosoroseus isolates showed the highest germination on CM and PDA media and the lowest on SDAY. However, some isolates presented high germination on nutrient-poor media (AW and MM.O presente trabalho objetivou investigar se meios de cultura utilizados em teste de viabilidade afetam a germinação de conídios de cinco isolados de Lecanicillium lecanii, cinco de Beauveria bassiana e quatro de Paecilomyces fumosoroseus. Os testes foram realizados em lâminas de microscopia contendo um dos seguintes meios de cultura: Ágar-água (AA, Meio Mínimo (MM, Batata, dextrose e ágar (BDA, Batata, dextrose, ágar e 1% de extrato de levedura (BDAL, Sabouraud, dextrose, ágar e extrato de levedura (SDAL e Meio Completo (MC. Delimitaram-se três áreas por lâmina e em cada uma aplicou-se 0,05mL de uma suspensão com concentração de 5,5 x 105 conídios ml-1. Para cada isolado foi realizado um bioensaio, com seis tratamentos e cinco repeti

  11. 安徽的虫草及其相关真菌I.%THE GENUS CORDYCEPS AND I0TS ALLIES FROM ANHUI I.

    Institute of Scientific and Technical Information of China (English)

    李春如; 樊美珍; 黄勃; 王四宝; 李增智

    2002-01-01

    报道疣孢虫草Cordyceps phymatospora为新种;球孢虫草C. bassiana 及其无性型Beauveria bassiana, 长座虫草C. longissima 及其无性型 Hirsutella longissima, 台湾虫草C. formosana 及其无性型 Hirsutella sp., 拟细虫草C. gracilioides和丝虫草C. filiformis 等19种虫草及其部分相关真菌.模式标本保存在安徽农业大学虫生菌研究中心(RCEFAAU).%19 species of Cordyceps and its allies from some nature preserves in Anhui Province, southeastern China are reported. They are Cordyceps phymatospora sp. nov., C. bassiana and its anamorph Beauveria bassiana, C. longissima and its anamorph Hirsutella longissima, C. formosana and its anamorph Hirsutella sp., C. gracilioides, C. filiformis and so on. Specimens examined are deposited in Research Center on Entomogenous Fungi, Anhui Agricultural University (RCEFAAU).

  12. Spatial and taxonomical overlap of fungi on phylloplanes and invasive alien ladybirds with fungal infections in tree crowns of urban green spaces

    DEFF Research Database (Denmark)

    Howe, Andrew Gordon; Ravn, Hans Peter; Jensen, Annette Bruun;

    2016-01-01

    ), Lecanicillium spp. (13.6% H. axyridis; 4.8% natives), with fewer Beauveria spp. infections (2.6% H. axyridis). Molecular identification revealed B. bassiana, B. pseudobassiana, I. farinosa and L. muscarium among isolates of both tree and ladybird origin. Tilia x europaea phylloplanes support a diverse...... occurred as infections in ladybirds. Isaria spp. was most abundant on phylloplanes (mean colony forming units (CFU) per leaf ± SE, 0.33 ± 0.03) followed by Beauveria spp. (0.22 ± 0.02 CFU per leaf) and Lecanicillium spp. (0.19 ± 0.02 CFU per leaf). Densities of inoculum were higher in inner crowns...... and decreased with height, although Lecanicillium spp. peaked at 5 – 7m. Upper phylloplane surfaces harboured higher densities of Isaria spp. and Beauveria spp. than lower surfaces, whereas Lecanicillium spp. was equally distributed. Most prevalent on ladybirds were Isaria spp. (20. 6% H. axyridis; 4.8% natives...

  13. Marcadores para la Realidad Aumentada para fines educativos

    OpenAIRE

    María Reina Zarate Nava; Cecilio Francisco Mendoza González; Honorato Aguilar Galicia; Juan Manuel Padilla Flores

    2013-01-01

    La Realidad Aumentada (AR) es considerada por algunos expertos como la tecnología en crecimiento para el 2014, en diferentes sectores: ingeniería, educación, juegos y negocios, siendo este último el más usado en él 2013. Sin embargo en el sector educativo la AR se presenta en algunos proyectos que se han desarrollado para diferentes áreas de las ciencias, como Química, Física y Matemáticas, lo anterior con el objetivo de facilitar el proceso de Enseñanza-Aprendizaje. En el presente artículo s...

  14. Taller para desarrollar la creatividad

    OpenAIRE

    MARTÍNEZ GUEROLA, BEATRIZ

    2015-01-01

    [ES] Partimos de la base de que la creatividad es una capacidad realmente valiosa, no solo para el ámbito artístico, si no para cualquier aspecto de la vida. Este proyecto está enfocado a un taller diseñado para que a partir de técnicas de expresión gráfico-plásticas podamos estimular y desarrollar la creatividad, principalmente en niños de tercer ciclo de primaria. Con este proyecto relacionamos el arte y la creatividad que se asocia a esté con la educación. Martínez Guerola, B. (2015). T...

  15. Para todo mal, a cura

    Directory of Open Access Journals (Sweden)

    Maria Borges

    2014-11-01

    Full Text Available Nesse artigo, eu apresento a teoria kantiana do mal. Mostrarei que Kant divide o mal em três níveis: fraqueza, impureza e perversidade, relacionando-os com afetos e paixões. Eu defendo que Kant apresenta várias formas de curar o mal nos diversos textos, tais como Doutrina da Virtude, Antropologia, Ideia para uma história universal do ponto de vista cosmopolita e Religião nos limites da simples razão. Eu tentarei mostrar que a virtude é impotente para curar o mal e que Kant apresenta uma comunidade ética para esse fim. Por fim, eu compararei a construção de comunidade ética com o estabelecimento de uma sociedade civil jurídica

  16. Instrucciones para los Autores

    Directory of Open Access Journals (Sweden)

    Revista Ciencias Biomedicas

    2014-04-01

    intereses debe estar especificada al final del artículo. También debe especificarse si se ha recibido dinero por concepto de financiación, especificando los orígenes y los códigos de las convocatorias donde se obtuvieron los recursos. De ser pertinente inmediatamente después pueden darse los agradecimientos respectivos a instituciones o personal que de otra manera aportaron al artículo y/o la investigación. 3. Políticas de arbitraje por pares. Los originales serán sometidos a un proceso de evaluación editorial que se cumplen en varias fases. Inicialmente los artículos recibidos son sometidos a un estudio preliminar por parte del director, editor o comité editorial quienes determinan la adecuada estructuración del manuscrito y su pertinencia con las políticas de la revista y de la institución. Si el artículo es aceptado por cumplir los requisitos formales y de contenido presentes en estas instrucciones, es enviado a dos pares académicos externos quienes de forma anónima realizan sugerencias y correcciones. Los pares evaluadores realizan su veredicto de la siguiente manera. (1 Publicar sin cambios. (2 publicar cuando se hayan cumplido las correcciones menores. (3 publicar cuando se hayan realizados correcciones de fondo y contenido. (4 no publicable. En caso de discrepancia en las opiniones será enviado a un tercer arbitro, de ser pertinente se solicitará la opinión a los miembro del comité científico. Los autores serán informados oportunamente de las diferentes fases que se cumplen en el proceso de evaluación editorial. Ellos recibirán las opiniones que han dado los pares evaluadores externos y siempre de forma anónima. También serán sometidos a evaluación por lingüística en español y en inglés. Al ser aprobada la publicación inmediatamente serán notificados los autores y se expedirá la certificación respectiva. Al estar disponibles las galeradas serán enviadas a los autores para evaluación y aprobación final.

  17. Interfaces no convencionales para juegos

    OpenAIRE

    Castro, Silvia Mabel; Larrea, Martín Leonardo; Martig, Sergio R.

    2005-01-01

    Además de los juegos para PCs de escritorio y consolas, hay numerosos juegos desarrollados para dispositivos móviles, tales como los celulares y las PALMs. Sin embargo, sólo unos pocos pueden sentir el ambiente físico. La mayoría de los juegos están atados al escritorio o a los dispositivos en los que el juego se ejecuta y son raros los que salen de allí, sin siquiera pensar en una experiencia que extienda considerablemente el área de juego. Esto plantea el desafío de nuevas tecnologías o ...

  18. Evaluación biológica del manejo de picudos y nematodos fitopatógenos en plátano (Musa AAB

    Directory of Open Access Journals (Sweden)

    Aristizábal Loaiza Manuel

    2009-12-01

    Full Text Available El trabajo se desarrolló en la granja Montelindo, municipio de Palestina (Caldas a 5° 05' N y 75° 40' O, a 1010 m.s.n.m., 23.5 °C, precipitación anual de 2100 mm y humedad relativa de 76%, con el fin de generar información sobre el manejo de picudos y nematodos fitoparásitos del plátano. Se usó un diseño en bloques completos al azar con cuatro tratamientos por bloque, tres repeticiones y 24 plantas por repetición. Para el manejo de los picudos se hicieron aplicaciones de Carbofurán, Beauveria bassiana y Metarhizium anisopliae en trampas tipo columna. Para el control de nematodos se hicieron aplicaciones en el suelo de Carbofurán y dos cepas comerciales de Paecilomyces lilacinus. Se evaluaron el número de adultos de picudos en trampas, la infección de estos por los hongos empleados y la población de nematodos en suelo y raíces. Se encontró que las trampas tratadas con Carbofurán fueron significativamente más efectivas para la captura de insectos. En laboratorio se estableció que M. anisopliae tuvo una mejor capacidad para infectar adultos del insecto en el campo. La población de nematodos fue menor en suelo y raíces de las plantas tratadas con Carbofurán. Paecilomyces lilacinus no fue efectivo para reducir las poblaciones de nematodos. Los géneros de nematodos predominantes fueron Radopholus, Pratylenchus, Meloidogyne y Helicotylenchus.

  19. Evaluación biológica del manejo de picudos y nematodos fitopatógenos en plátano (Musa AAB

    Directory of Open Access Journals (Sweden)

    Carolina González Cardona

    2009-10-01

    Full Text Available El trabajo se desarrolló en la granja Montelindo, municipio de Palestina (Caldas a 5° 05' N y 75° 40' O, a 1010 m.s.n.m., 23.5 °C, precipitación anual de 2100 mm y humedad relativa de 76%, con el fin de generar información sobre el manejo de picudos y nematodos fitoparásitos del plátano. Se usó un diseño en bloques completos al azar con cuatro tratamientos por bloque, tres repeticiones y 24 plantas por repetición. Para el manejo de los picudos se hicieron aplicaciones de Carbofurán, Beauveria bassiana y Metarhizium anisopliae en trampas tipo columna. Para el control de nematodos se hicieron aplicaciones en el suelo de Carbofurán y dos cepas comerciales de Paecilomyces lilacinus. Se evaluaron el número de adultos de picudos en trampas, la infección de estos por los hongos empleados y la población de nematodos en suelo y raíces. Se encontró que las trampas tratadas con Carbofurán fueron significativamente más efectivas para la captura de insectos. En laboratorio se estableció que M. anisopliae tuvo una mejor capacidad para infectar adultos del insecto en el campo. La población de nematodos fue menor en suelo y raíces de las plantas tratadas con Carbofurán. Paecilomyces lilacinus no fue efectivo para reducir las poblaciones de nematodos. Los géneros de nematodos predominantes fueron Radopholus, Pratylenchus, Meloidogyne y Helicotylenchus.

  20. Espacio de trabajo colaborativo para emprendedores, Pinedo

    OpenAIRE

    FERRIZ NAVARRO, ANDRÉS

    2016-01-01

    Espacio de trabajo colaborativo (CoWorking) para emprendedores en Pinedo. Ferriz Navarro, A. (2014). Espacio de trabajo colaborativo para emprendedores, Pinedo. http://hdl.handle.net/10251/67050. Archivo delegado

  1. MODELO PARA ESTABLECER SANCIONES PECUNIARIAS PARA DELITOS AMBIENTALES

    Directory of Open Access Journals (Sweden)

    LUIS DIEGO VÉLEZ GÓMEZ

    2012-01-01

    Full Text Available Este artículo pretende exponer el modelo básico que sustenta el desarrollo de multas óptimas; en él se muestra la forma de desincentivar los intereses del violador de la normatividad ambiental, e igualmente el método para alcanzar efectos compensatorios sobre los daños cometidos al medio ambiente. El desarrollo del tema sobre sanciones pecuniarias (multas y penalidades (encarcelamiento toma la relación contractual entre el agente (la empresa y el principal (La entidad reguladora para, a través de esta relación, entrar en la determinación de un diseño eficaz de sanciones. Se utilizara el modelo de agente-principal para examinar los efectos de las sanciones corporativas e individuales sobre los incentivos de cumplimiento en el contexto ambiental; además se emplearán los conceptos de bienes públicos, bienes comunes y externalidades como categorías de análisis que permiten la identificación y valoración de daños ambientales.

  2. Nacidos para curar...y vivir.

    Directory of Open Access Journals (Sweden)

    Pedro A Marina González

    2009-01-01

    Full Text Available Se presentan argumentos para el debate acerca del impacto para la sociedad y el individuo de las técnicas de reproducción que permiten el “diseño” genético de bebes para el tratamiento de hermanos con enfermedades incurables.

  3. Ocio Inclusivo para personas con discapacidad

    OpenAIRE

    Ruiloba Arce, Marta María

    2014-01-01

    Mi proyecto de Fin de Grado trata del ocio inclusivo para personas con discapacidad intelectual. Como educadora social apuesto por la inclusión social para la mejora de calidad de vida de las personas, siendo uno de los medios más efectivos para lograr una sociedad sin diferencias ni prejuicios. [...

  4. Uma taxonomia para modelos multiparadigma

    OpenAIRE

    Barbosa, Jorge L. V.; Geyer, Cláudio Fernando Resin

    1998-01-01

    Desde os primórdios da humanidade a raça humana busca compreender. A ciência, como instrumento de descobrimento do universo, vem alcançando contínuos sucessos. As descobertas científicas permitiram ao Homem criar um modelo bastante estável e previsível para a realidade na qual está inserido. Em grande parte, o sucesso da ciência basea-se na estratégia de fragmentação do conhecimento, ou seja, na criação de diversas áreas de especialização. A fragmentação é uma solução natural para resolver pr...

  5. Separating Para and Ortho Water

    CERN Document Server

    Horke, Daniel A; Długołęcki, Karol; Küpper, Jochen

    2014-01-01

    Water exists as two nuclear-spin isomers, para and ortho, determined by the overall spin of its two hydrogen nuclei. For isolated water molecules the conversion between these isomers is forbidden and they act as different molecular species. Yet, these species are not readily separable, and little is known about their specific physical and chemical properties, conversion mechanisms, or interactions. Here we demonstrate the production of isolated samples of both spin isomers in pure beams of para and ortho water, with both species in their respective absolute ground state. These single-quantum-state samples are ideal targets for unraveling spin-conversion mechanisms, for precision spectroscopy and fundamental-symmetry-breaking studies, and for spin-enhanced applications, e. g., laboratory astrophysics and -chemistry or hypersensitized NMR experiments.

  6. Argumentos para repensar el "desarrollo"

    Directory of Open Access Journals (Sweden)

    María Luisa Eschenhagen

    2001-06-01

    Full Text Available Con este trabajo se quiere hacer una aproximación al problema desde una perspectiva diferente, considerando nuevos argumentos que hasta hace muy poco tiempo no se habían tenido en cuenta y que se vienen formulando de una manera muy interesante en los últimos años. El objetivo del artículo es presentar tres argumentos para señalar la necesidad de repensar, desde sus fundamentos, el concepto de desarrollo.

  7. para asegurar un hijo sano

    Directory of Open Access Journals (Sweden)

    Carmen Amarilis Guerra de Castillo

    2006-01-01

    Full Text Available Esta investigación etnográfica focalizada exploró las creencias, los valores, los hábitos y los padrones comportamentales de las embarazadas diabéticas atendidas en el Hospital Doctor Enrique Tejera, quienes residen en tres comunidades urbanas pobres de la ciudad de Valencia - Venezuela, con el objetivo de encontrar el significado del cuidado de si para dichas grávidas. Se utilizó como referencial teórico el Modelo del Análisis de la Salud y la Teoría Antropológica de la Salud, siendo fundamentales para revelar aquello que estaba implícito en el comportamiento de las mujeres con relación a su cuidado. Surgieron tres temas: la salud y la enfermedad - valores culturales del cuidado de si; las prácticas del cuidado de la salud y cuidándose en el embarazo garantiza tener un hijo más saludable. Los temas culturales revelaron que las creencias, los valores, los hábitos y los patrones culturales comportamentales, conforme las tres maneras de acción identificadas, pueden ser preservadas, acomodadas y re-estructuradas para proporcionar un cuidado culturalmente congruente.

  8. Estrategia para la sustentabilidad ambiental

    Directory of Open Access Journals (Sweden)

    Cecilia Erbiti

    2006-01-01

    Full Text Available En el contexto de los postulados conceptuales y metodológicos de la planificación estratégica y de sustentabilidad ambiental, el objetivo de esta investigación es describir el proceso de formulación del Plan de Ordenamiento Territorial del Municipio de Tandil (POTM y analizar su potencialidad para avanzar hacia la sustentabilidad ambiental del sistema. Los resultados de la misma muestran que el POTM no sólo prioriza principios, objetivos y estrategias que materializan las diferentes manifestaciones de la sustentabilidad (económica, social, ecológica y política, sino que la totalidad del Plan se fundamenta en el concepto de desarrollo sostenible y, con ello, se establecen prioridades de actuación que posibilitarán la gestión ambiental urbana. Si bien el POTM muestra una gran potencialidad para avanzar hacia la sustentabilidad del territorio, la implementación y cumplimiento del mismo constituye un fuerte desafío para las autoridades de aplicación.

  9. PLATAFORMA PARA LA VENTA DE VIDEOJUEGOS DIGITALES PARA PC

    OpenAIRE

    Gómez-Eguílar, Javier

    2013-01-01

    A lo largo del trabajo vamos a exponer nuestra propia idea de negocio de una nueva empresa, llamada GAMERINET, que se dedique a la venta de videojuegos de ordenador en descarga digital a través de nuestra web. Para ello vamos a ver una breve introducción a este sector de negocio y su historia, haremos un análisis de las condiciones actuales del sector, a través del análisis del entorno específico (5 Fuerzas de Porter) y del análisis del entorno general (Análisis PEST). Posterio...

  10. Occurrence of entomopathogenic fungi from agricultural and natural ecosystems in Saltillo, México, and their virulence towards thrips and whiteflies.

    Science.gov (United States)

    Sánchez-Peña, Sergio R; Lara, Jorge San-Juan; Medina, Raúl F

    2011-01-01

    Entomopathogenic fungi were collected from soil in four adjacent habitats (oak forest, agricultural soil, pine reforestation and chaparral habitat) in Saltillo, México using the insect bait method with Tenebrio molitor (L.) (Coleoptera: Tenebrionidae) larvae as bait. Overall, of the larvae exposed to soil, 171 (20%) hosted Beauveria bassiana (Balsamo) Vuillemin (Hypocreales: Cordycipitaceae), 25 (3%) hosted Metarhizium anisopliae (Metschnikoff) Sorokin (Hypocreales: Clavicipitaceae) and 1 (0.1%) hosted lsaria (=Paecilomyces) sp. (Hypocreales: Cordycipitaceae). B. bassiana was significantly more frequent on larvae exposed to oak forest soil. M. anisopliae was significantly more frequent on larvae exposed to agricultural soil. From the infected bait insects, 93 isolates of B. bassiana and 24 isolates of M. anisopliae were obtained. Strains were tested for their infectivity against Cuban laurel thrips, Gynaikothrips uzeli Zimmerman (Thysanoptera: Phlaeothripidae) and the greenhouse whitefly, Trialeurodes vaporariorum (Westwood) (Hemiptera: Aleyrodidae). B. bassiana isolates caused the highest mortality on thrips (some causing 88% mortality after 6 days); both fungal species caused similarly high mortality levels against whiteflies (75%) after 6 days. Large amounts of germplasm of entomopathogenic fungi, fundamentally B. bassiana and M. anisopliae, exist in the habitats sampled; pathogenicity varied among strains, and some strains possessed significant virulence. Soils in these habitats are reservoirs of diverse strains with potential for use in biocontrol.

  11. Bombas extractoras para lactancia materna

    OpenAIRE

    Rozas García, M. Rosa

    1997-01-01

    Durante la lactancia materna pueden utilizarse diferentes accesorios para complementarla, entre los cuales se encuentran las bombas extractoras de leche materna. Describiremos las características generales de los diferentes tipos de bombas extractoras, de modo que el profesional de enfermería conozca no sólo el funcionamiento de los aparatos, sino que también adquiera unos conocimientos acerca de sus indicaciones, manipulación, ventajas e inconvenientes, y pueda asesorar en todo momento a la ...

  12. Ambiente Java para sistemas embedded

    OpenAIRE

    Silva, Valter Filipe Miranda Castelão da

    2002-01-01

    Os sistemas integrados, ou genericamente os sistemas com reduzida capacidade de processamento e pouca memória, são actualmente sistemas economicamente acessíveis e muito divulgados. Os seus sistemas operativos estão, no entanto, ainda bastante dependentes do hardware o que faz que cada aplicação seja especialmente desenvolvida para cada tipo de sistema. Neste documento discute-se a aplicabilidade de uma máquina virtual Java em equipamentos com estas restrições. É proposta a ...

  13. Tendiendo puentes para la convivencia

    OpenAIRE

    Marcos Aranda, Rafael

    2006-01-01

    El Foro Iberoamericano sobre Seguridad Ciudadana, Violencia Social y Políticas Públicas, que se celebró en Madrid y Alcalá de Henares, entre los días 19 y 23 de junio del presente año, concluyó con la presentación de un documento de compromiso titulado «Tendiendo puentes para la convivencia». Dicho documento puede calificarse de mínimos, aunque en él se establecen una serie de consensos básicos, los cuales nos invitan a reflexionar sobre una realidad terriblemente complej...

  14. Manual de mercadeo para pymes

    OpenAIRE

    Botero Ardila, José Luis

    2012-01-01

    Este texto presenta las definiciones y lineamientos a seguir por parte de los empresarios, con el fin de hacerlos más competitivos a la hora de enfrentar las variaciones del mercado o del entorno competitivo. Su diseño tiene en cuenta al empresario que no dispone de mucho tiempo para aplicar el mercadeo en su empresa, por lo cual incluye la posibilidad de diagnosticar de manera autónoma el estado de la misma. Entre los temas que encontrará el lector se encuentran: ¿qué es mercadeo, investigac...

  15. Pequenos para idade gestacional: fator de risco para mortalidade neonatal

    Directory of Open Access Journals (Sweden)

    Almeida Márcia Furquim de

    1998-01-01

    Full Text Available OBJETIVO: Estudar as variáveis contempladas na Declaração de Nascimento (DN como possíveis fatores de risco para nascimentos pequenos para a idade gestacional (PIG e o retardo de crescimento intra-uterino como fator de risco para a mortalidade neonatal. MATERIAL E MÉTODO: As variáveis existentes na DN foram obtidas diretamente de prontuários hospitalares. Os dados referem-se a uma coorte de nascimentos obtida por meio da vinculação das declarações de nascimento e óbito, correspondendo a 2.251 nascimentos vivos hospitalares, de mães residentes, ocorridos no Município de Santo André, Região Metropolitana de São Paulo, no período de l/1 a 30/6/1992, e aos óbitos neonatais verificados nessa coorte. RESULTADOS: Obteve-se a proporção de 4,3% de nascimentos PIG, significativamente maior entre os recém-nascidos de pré-termo e pós-termo, entre os nascimentos cujas mães tinham mais de 35 anos de idade e grau de instrução inferior ao primeiro grau completo. Os recém-nascidos PIG apresentam maior risco de morte neonatal que aqueles que não apresentavam sinais de retardo de crescimento intra-uterino. CONCLUSÕES: Em áreas com menor freqüência de baixo peso ao nascer, é importante investigar a presença de retardo de crescimento intra-uterino entre os nascimentos prematuros e não apenas nos nascimentos de termo. O registro da data da última menstruação (ou da idade gestacional em semanas não agregadas na DN facilitaria a detecção de PIGs na população de recém-nascidos.

  16. Spectral Analysis Code: PARAS SPEC

    CERN Document Server

    Chaturvedi, Priyanka; Anandarao, B G

    2016-01-01

    The light emitted from the stellar photosphere serves as a unique signature for the nature of stars. The behaviour of these stellar lines depend upon the surface temperature, mass, evolutionary status and chemical composition of the star. With the advent of high-resolution spectrographs coupled with medium to large aperture telescopes around the globe, there is plenty of high-resolution and high signal-to-noise ratio data available to the astronomy community. Apart from radial velocity (RV) studies, such data offer us the unique opportunity to study chemical composition and atmospheric properties of the star. The procedure used to derive these parameters must be automated and well adaptable to data available from any high-resolution spectrograph. We hereby present an IDL code, PARAS SPEC, which was primary designed to handle high-resolution spectroscopy data from PARAS spectrograph coupled with the 1.2~m telescope at Mt. Abu, India. This code is designed to adapt with data from other spectrographs as well. Th...

  17. Edificio para viviendas, en Barcelona

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    Barba Corsini, F.

    1967-05-01

    Full Text Available This building has a garage basement, a ground floor with a communed playing zone for all the children of the block, and the entrance vestibule. The rest of the building is occupied with apartments, of about 200 m2 each, which are functionally designed, and are suitable for medium sized average income families. Special attention has been given to the external facades, which, among other things, are of extremely simple design.El edificio contiene: en el sótano, un guardacoches; en la planta baja, una zona de juegos común para los niños de todas las viviendas, además de los vestíbulos. El resto de la superficie edificada está destinado a viviendas, de unos 200 m2, construidos con distribución funcional y adecuada para familias medias ya constituidas. Fue dedicada una especial atención a la composición de las fachadas, que ofrecen, entre otros atractivos, el de su elegante sencillez.

  18. Internet como instrumento para la Yihad

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    Alfonso Merlos García

    2006-01-01

    Full Text Available Las extraordinarias ventajas que ofrece Internet lo han convertido en un instrumento clave y dinámico en la estrategia de los yihadistas. Los medios de comunicación, la clase política y las agencias de seguridad e información han tendido a concentrar sus esfuerzos en la neutralización del ciberterrorismo sin prestar suficiente atención a los múltiples usos de Internet que están haciendo los neosalafistas: para la propaganda y para la guerra psicológica, para el reclutamiento y para la financiación, para la documentación y para la planificación de atentados. Las democracias deberían considerar el coste que tendrá la derrota frente al movimiento yihadista globalizado en la batalla por el ciberespacio

  19. Aprender para la vida o para el placer

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    Diobaldo César Heredia Gutiérrez

    2011-07-01

    Full Text Available El proceso enseñanza aprendizaje, se corresponde con un sistema comunicativo por excelenciaque requiere una alta dosis de amor que garantice el disfrute o goce de los participantes, enun interactuar de búsqueda y descubrimiento satisfactorio. Para imprimirle un alto grado de calidad, esdeseable la participación de estudiantes y docentes más emprendedores y críticos y menos académicoso dependientes, por ello el aprendizaje autónomo es clave, el logro u objetivo, entonces, es estudiantesque aprendan como si fueran a enseñar y docentes investigadores, proactivos y expertos en las materiasque enseñan.

  20. Inteligencia Emocional para tiempos de crisis

    OpenAIRE

    Fernandez-Berrocal, Pablo

    2014-01-01

    La Inteligencia Emocional se concibe como un conjunto de habilidades, tanto básicas como complejas, dirigidas a “unificar las emociones y el razonamiento”, esto es, usar las emociones para facilitar el razonamiento y los procesos de pensamiento, y usar nuestro razonamiento para pensar de forma inteligente acerca de nuestras emociones (Mayer y Salovey, 1997). En concreto, la IE se define como una habilidad mental que incluye “la capacidad para percibir, valorar y expresar las emociones con...

  1. Paisajes para un desarrollo sustentable y participativo

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    Rafael Mata Olmo

    2014-11-01

    Para terminar, el texto recoge una experiencia de proyecto territorial de paisaje, la del Plan Insular de Menorca (Islas Baleares, España, aprobado en 2003. Se sintetizan sus determinaciones sobre la protección de determinados terrenos por sus altos valores paisajísticos, sus directrices para que las políticas sectoriales (turismo, agricultura, infraestructuras, etc. incorporen criterios paisajísticos, y sus iniciativas para la gestión y mejora del paisaje y para el fomento del acceso público a su contemplación y disfrute.

  2. Adesao ao guia alimentar para populacao brasileira

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    Eliseu Verly Junior

    2013-12-01

    Full Text Available OBJETIVO : Analisar a adesão ao Guia Alimentar para População Brasileira. MÉTODOS : Amostra composta por participantes do Inquérito de Saúde de São Paulo (n = 1.661 que preencheram dois recordatórios de 24 horas. Foi utilizado modelo bivariado de efeito misto para a razão entre o consumo de energia do grupo de alimentos e o consumo calórico total. A razão estimada foi utilizada para calcular o percentual de indivíduos com consumo abaixo ou acima da recomendação. RESULTADOS : Pelo menos 80,0% da população consome abaixo do recomendado para: leite e derivados; frutas e sucos de frutas; e cereais, tubérculos e raízes; aproximadamente 60,0% para legumes e verduras; 30,0% para feijões; e 8,0% para carnes e ovos. Adolescentes apresentaram a maior inadequação para legumes e verduras (90,0%, e o estrato de maior renda foi associado à menor inadequação para óleos, gorduras e sementes oleaginosas (57,0%. CONCLUSÕES : Foi observado consumo inadequado dos grupos de alimentos relacionados com aumento do risco de doenças crônicas.

  3. Evaluación biológica del manejo de picudos y nematodos fitopatógenos en plátano (Musa AAB Biological evaluation of the management of borers and phytopathogenic nematodes of plantain (Musa AAB

    Directory of Open Access Journals (Sweden)

    Carolina González Cardona

    2009-10-01

    Full Text Available El trabajo se desarrolló en la granja Montelindo, municipio de Palestina (Caldas a 5° 05' N y 75° 40' O, a 1010 m.s.n.m., 23.5 °C, precipitación anual de 2100 mm y humedad relativa de 76%, con el fin de generar información sobre el manejo de picudos y nematodos fitoparásitos del plátano. Se usó un diseño en bloques completos al azar con cuatro tratamientos por bloque, tres repeticiones y 24 plantas por repetición. Para el manejo de los picudos se hicieron aplicaciones de Carbofurán, Beauveria bassiana y Metarhizium anisopliae en trampas tipo columna. Para el control de nematodos se hicieron aplicaciones en el suelo de Carbofurán y dos cepas comerciales de Paecilomyces lilacinus. Se evaluaron el número de adultos de picudos en trampas, la infección de estos por los hongos empleados y la población de nematodos en suelo y raíces. Se encontró que las trampas tratadas con Carbofurán fueron significativamente más efectivas para la captura de insectos. En laboratorio se estableció que M. anisopliae tuvo una mejor capacidad para infectar adultos del insecto en el campo. La población de nematodos fue menor en suelo y raíces de las plantas tratadas con Carbofurán. Paecilomyces lilacinus no fue efectivo para reducir las poblaciones de nematodos. Los géneros de nematodos predominantes fueron Radopholus, Pratylenchus, Meloidogyne y Helicotylenchus.This work was carried out at the ‘Montelindo’ farm, municipality of Palestina (Department of Caldas, Colombia, located at 5° 05' N and 75° 40' W, at 1010 m.a.s.l., 23.5 °C, with 2100 mm of annual rainfall, and relative humidity of 76%, in order to generate information on the management of borers and parasitic nematodes of the plantain. A completely randomised block experimental design was used, with four treatments per block, three replicates and 24 plants per replicate. For the management of borers, applications of Carbofuran, Beauveria bassiana and Metarhizium anisopliae were made

  4. Entre velos: Maya para contrabajo

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    Rosa María Rodríguez Hernández

    2014-01-01

    Full Text Available Maya para contrabajo se materializa a partir de una imagen de Pepe Romero, cuyo texto interpreta a La decadencia de la mentira de Oscar Wilde. La estructura de Maya es binaria, especular y asimétrica; su poética obedece al juego verdad/mentira que subyace en la obra artística y en la vivencia del artista. Maya trae a la memoria las voces de los Upanishad, Schopenhauer, Shakespeare y Beethoven, influencias éstas en la obra de Wagner y, todas ellas, en la de Eliot. Los aspectos gestuales y visuales de Maya entrelazan con la danza de Pina Bausch y con la retórica (hypotiposis.

  5. Una minga para el posdesarrollo

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    Arturo Escobar

    2011-01-01

    Full Text Available El artículo sintetiza algunas conclusiones a las que han llegado movimientos sociales y académicos en las últimas dos décadas al reflexionar sobre la globalización y sus implicaciones; el modelo dominante, basado en las nociones de "desarrollo" y "modernidad", ha entrado en crisis. Por lo tanto, se hace necesario avanzar en una transición cultural que se pueda comprender como un cambio de época y no sólo como una época de cambios. Para llevar a cabo esta transformación, se deben resolver las con-tradicciones existentes entre el neodesarrollismo y el postdesarrollo, por supuesto, a favor de éste último.

  6. para su puesta en marcha

    Directory of Open Access Journals (Sweden)

    Sergio Franco Maass

    2004-01-01

    Full Text Available Los sistemas de información geográfica (SIG tienen una gran aplicación en la gestión municipal; permiten cumplir con dos objetivos fundamentales: el registro de información territorial y el análisis de problemas territoriales específicos. Con el fin de determinar la viabilidad de este tipo de tecnologías en el ámbito municipal, se enfrentó el problema de la localización de instalaciones para la disposición final de residuos sólidos urbanos en el municipio de Toluca, Estado de México. Se identifican ciertos aspectos que limitan la práctica de los SIG: la diversidad entre los municipios, los problemas administrativos, las deficiencias de la información y la carencia de recursos humanos, técnicos y financieros.

  7. Biodegradability of chitosan-polyvinylalcohol-acrylic acid superabsorbent polymers%壳聚糖-聚乙烯醇-丙烯酸高吸水树脂的生物降解性能研究

    Institute of Scientific and Technical Information of China (English)

    陈嘉恒; 吴国杰; 廖宗祺; 陈倩瑜

    2013-01-01

    采用培养基培养方法,考察了黑曲霉(Aspergillus flavus)和白僵菌(Beauveria bassiana)利用壳聚糖-聚乙烯醇-丙烯酸高吸水树脂中碳源的生长情况,并对高吸水树脂作为未来生物农药白僵菌的载体进行了初步探索。扫描电镜分析表明,壳聚糖-聚乙烯醇-丙烯酸高吸水树脂具有一定的生物降解性。%The growth of Aspergillus flavus and Beauveria bassiana was examined in the medium with the carbon source of Chitosan-polyvinylalcohol-Acrylic acid Superabsorbent Polymers .The use of superabsor-bent polymers as a vector for biopesticide beauveria bassiana was prospected .The scanning electron mi-croscope analysis showed that Chitosan-polyvinylalcohol-Acrylic acid Superabsorbent Polymers possesses certain biodegradability .

  8. The para-HK/QK correspondence

    CERN Document Server

    Dyckmanns, Malte

    2016-01-01

    We generalise the hyper-Kahler/quaternionic Kahler (HK/QK) correspondence to include para-geometries, and present a new concise proof that the target manifold of the HK/QK correspondence is quaternionic Kahler. As an application, we construct one-parameter deformations of the temporal and Euclidean supergravity c-map metrics and show that they are para-quaternionic Kahler.

  9. Trabalhar para estudar/estudar para trabalhar: realidade e possibilidades

    Directory of Open Access Journals (Sweden)

    Janes Teresinha fraga Siqueira

    2011-01-01

    Full Text Available O trabalho que apresentamos é parte da pesquisa, ainda não concluída, com os estudantes das licenciaturas e da pedagogia da Universidade de Santa Cruz do Sul- UNISC-RS-Brasil. Este está inserido na linha de pesquisa: educação, trabalho e emancipação do mestrado em educação desta Universidade. Queremos compreender, à luz do materialismo histórico e dialético e das categorias da crítica da economia política, os significados atribuídos pelos estudantes universitários ao fenômeno trabalho e estudo. É uma pesquisa preferentemente qualitativa de natureza dialética. Para Marx, a essência da realidade humana reside no trabalho, mas a fonte de toda a riqueza está na natureza. Logo trabalho, natureza e sociedade estão em relação dialética. Trabalho, portanto, é a ideia central articuladora, e natureza e sociedade devem ser estudadas em conexão com o trabalho. Marx trata o trabalho, no modo de produção capitalista, como um impedimento ao desenvolvimento humano. Propõe omesmo como um ato de criação e auto-expressão humana que não deve ter um valor. A compreensão dessa premissa é necessária para que os trabalhadores possam significar e ressignificar o trabalho para além da ideologia dominante. Algumas categorias aparecem como relevantes para a análise: condições de trabalho e de estudo, necessidade de formação, predominância do trabalho sobre o estudo, dificuldade deconciliar tempo de estudo e de trabalho. Na relação com o objeto de estudo, ressaltaremos a crise estrutural do capital, a desregulamentação das leis do trabalho e sua flexibilização e as contradições entre trabalho e estudo bem como quais possibilidades emancipatórias são visualizadas ou sonhadas pelos trabalhadores estudantes.Resume Ce travail constitue une partie de la recherche entreprise avec les étudiants du cours de pédagogie de l´Universidade de Santa Cruz do Sul – UNISC – RS – Brésil. Il est intégré dans le champ de

  10. Recursos para un aprendizaje significativo

    Directory of Open Access Journals (Sweden)

    Juan LARA GUERRERO

    2009-11-01

    Full Text Available RESUMEN: Partimos del supuesto de que una de las cuestiones que más preocupa al profesorado en su práctica educativa radica en que el aprendizaje de sus alumnos sea significativo. Somos conscientes de que, para conseguir este tipo de aprendizaje, es necesario introducir una serie de cambios en la dinámica de la clase. Comenzamos diferenciando el aprendizaje maquinal del significativo. Continuamos aclarando una confusión muy extendida entre muchos educadores, que creen que el aprendizaje por recepción es repetitivo y que el efectuado por descubrimiento es forzosamente significativo. Posteriormente, planteamos una serie de interrogantes para aclarar algunas cuestiones que le pueden ser útiles a los educadores en su práctica cotidiana. Terminamos presentando la estrategia de organización, que cuenta con dos instrumentos, a nuestro juicio muy eficaces, para ayudar a los estudiantes, a aprender de manera significativa: los mapas conceptuales y la técnica heurística V.ABSTRACT: We start from the assumption that one of the questions that most worry the teaching staff in its educational practice consists in that its pupils' learning is significant. We are aware that, to obtain this kind of learning, it is necessary to introduce a series of changes in the classroom dynamics. We begin by making a distinction between mechanical and significant learning. We continué making clear a very extended confusión amongst many teachers, who believe that learning by reception is repetitive and that the one done by serendipity is necessarily significant. Afterwards, we créate a series of questions to clarify with some answers that can be useful to teachers in their everyday practice. We finish showing the organization strategy, which has two instruments, that in our opinión are very effective, to help students learning in a significant way: conceptual maps and the V heuristic technique.RÉSUMÉ: Nous partons de la supposition de qu'une des questions que

  11. Una red para la gente

    Directory of Open Access Journals (Sweden)

    Raúl Trejo Delarbre

    2004-02-01

    Full Text Available La próxima Cumbre Mundial de la Sociedad de la Información ha revitalizado, en numerosos países, el debate acerca de las nuevas tecnologías de la comunicación y los usos que pueden tener. Lamentablemente en México hemos estado casi del todo ajenos a esa intensa, extensa y en otras latitudes fructífera discusión. Ni los especialistas interesados en estos temas, ni la sociedad que es a la postre beneficiaria o damnificada según sea el empleo de tales tecnologías, ni los organismos del Estado a cargo de tales asuntos, han propiciado la reflexión colectiva que nos podría permitir tanto afinar las posiciones de nuestro país rumbo a la cumbre de Ginebra y Túnez como deliberar, en ese contexto, acerca de lo mucho que nos falta para tener un país auténticamente imbricado en la construcción de una auténtica Sociedad de la Información.

  12. Residencia para familias - Anglet (Francia

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    Hébrard, J. R.

    1971-11-01

    Full Text Available This residential hotel has 806 beds, which can be increased to 1,100 by adding some double beds. Its size and colour blends well with the picturesque surrounding, known as «Chambre d'Amour». The hotel involves 120 large apartments, and 50 smaller one, as well as 25 rooms for the staff, and flats for the manager and other senior officials. There is a hall, large living room, bar, rooms for playing games, nursery, and an additional hostel with 40 flats and staff rooms. The design is of a linear, continuous style, emphasizing its horizontal extension, though contrast is provided by a higher block, housing the vertical circulation facilities.Con un total de 806 plazas hoteleras, ampliables a 1.100 (si se utilizan literas, este conjunto, por su volumen y color se integra en el pintoresco paisaje del paraje conocido por «Chambre d'Amour». Comprende: 120 apartamentos; 50 más pequeños; 25 habitaciones para empleados; los apartamentos del director, gobernanta y recepcionistas; las zonas de vida en común: hall, salón, bar, sala de juegos y guardería; un albergue con 40 apartamentos; y las dependencias de la administración. Presenta un volumen lineal y continuo, modelando una composición de ritmo horizontal predominante, a la que le imprime gran variedad el cuerpo vertical de hormigón visto que contiene las circulaciones verticales.

  13. Control biológico en el cultivo del arroz en Cuba. (Resultados 1970 – 2005.

    Directory of Open Access Journals (Sweden)

    Rafael Meneses

    2007-12-01

    Full Text Available El cultivo del arroz en Cuba está afectado por diversas plagas, fundamentalmente: Tagosodes orizicolus, Lissorhoptrus brevirostris, Oebalus insularis y Spodoptera frugiperda. A partir de 1970 se iniciaron los estudios sobre los principales controles biológicos de estas plagas. Para T. orizicolus se ha determinado que Paranagrus perforator y Tytthus parviceps son los principales enemigos naturales de esta plaga. El porcentaje de huevos de T. orizicolus parasitados por P. perforator ha llegado al 50.7% en la etapa de germinación a ahijamiento activo de la planta de arroz. Además, a esta plaga se le ha detectado control por los parasitoides Elenchus sp. y Gonatopus sp. L. brevirostris ha sido manejado en los arrozales con los hongos Metarhizium anisopliae y Beauveria bassiana, obteniendo muy buenos resultados, tanto en el control como en las afectaciones ocasionadas por este insecto. La aplicación de M. anisopliae cepa Niña Bonita resulta satisfactorio para el control de O. insularis. Se ha determinado diversos depredadores, parasitoides y hongos que en determinadas épocas del año ejercer buen control de S. frugiperda, destacándose dentro de ellos: Coleomegilla cubensis, Chelonus texanus y Telenomus spp. C. texanus ha presentado un nivel de parasitismo superior al 80.0 % y Telenomus sp. de 93 %, en condiciones de producción arrocera. Con la presencia de arañas en los arrozales se logra disminuir considerablemente las poblaciones de insectos plagas.

  14. Inferencia bayesiana para algunas leyes de Lanchester

    OpenAIRE

    Wiper, Michael P.; Pettit, L. I.; Young, K. D. S.

    2000-01-01

    Lanchester (1916) introdujo una serie de ecuaciones o leyes determinísticas para modelar los números de bajas en un combate sin refuerzos. Un inconveniente de estos modelos es que no son útiles ni para la inferencia ni para la predicción, ya que el resultado de una batalla viene determinado por los parámetros del sistema y los tamaños iniciales de cada ejército. Se han propuesto varias versiones estocásticas de las ecuaciones de Lanchester. En este artículo, se resumen algunos de estos modelo...

  15. Estereoscopio com tela holografica para ver tomografias

    CERN Document Server

    Lunazzi, J J; Magalhaes, D S F

    2010-01-01

    A estereoscopia eh uma tecnica que permite a observacao de imagens tridimensionais, mas estah sempre associada com o uso de algum equipamento especial para visualizacao, como oculos bicolores ou polarizados. Para o uso em aplicacoes medicas o emprego de tais equipamentos pode inviabilizar sua utilizacao durante procedimentos cirurgicos, por exemplo. Neste trabalho apresentamos um novo tipo de estereoscopio que utiliza uma tela holografica para geracao de imagens tridimensionais sem o uso de qualquer equipamento adicional. Apresentamos a descricao do equipamento utilizado e resultados das imagens visualizadas.

  16. Test para la profilaxis de la prematuridad

    OpenAIRE

    Cruz Laguna, Gladys María

    2008-01-01

    La prematuridad constituye un problema mundial de salud, la comunidad científica busca predictores para disminuir su incidencia. La investigación persiguió obtener una escala de puntuación que modele las características cervicales y permita diagnosticar la condición de riesgo de las gestantes para extender el período de gestación y lograr nacimientos al término. Se realizó un cuasiexperimento en gestantes con factores de riesgo para la prematuridad del municipio Holguín. La muestra fue confor...

  17. Biocomercio: una alternativa para el desarrollo sostenible

    OpenAIRE

    Murillo R., Carlos; Arias Ramírez, Rafael

    2008-01-01

    El desarrollo sostenible en países ricos en biodiversidad dependerá de la capacidad de los gobiernos de implementar los arreglos institucionales necesarios para conciliar la preservación de los recursos naturales con el uso de los mismos para la producción de bienes y servicios que puedan potenciar el crecimiento económico y un mayor bienestar para las comunidades locales donde estos recursos se encuentren. Este es el objetivo de la Iniciativa de Biocomercio que ha venido impulsando la Confer...

  18. Domótica para viviendas construidas

    OpenAIRE

    Beaskoetxea Gartzia, Unai

    2011-01-01

    El objeto de este proyecto fin de carrera es mostrar las distintas tecnologías disponibles para la domótica actual, y cómo se podría aplicarlas en una vivienda ya construida, incidiendo en el uso de la tecnología ZigBee, aunque también se combinará con el uso de KNX, RF, etc. Este proyecto presentará 3 niveles de domótica: un primer nivel para personas sin discapacidad, un segundo nivel para personas de edad avanzada, y un tercer nivel adaptado a personas con discapacidad. Se indicará...

  19. Los videojuegos: conectar alumnos para aprender

    OpenAIRE

    José Antonio Valderrama Ramos

    2012-01-01

    Este artículo explora la utilidad que presentan los videojuegos comerciales actuales para educar a los jugadores y ayudarlos a adquirir habilidades necesarias para desempeñarse mejor en nuestra sociedad. Aborda el problema de la educación formal, la cual no está respondiendo a la situación de complejidad de nuestro entorno y usa el paradigma del pensamiento complejo y siete saberes para la educación propuestas por Edgar Morin. También, describe algunas ideas que le pueden ser de utilidad a do...

  20. Una arquitectura unificada para el razonamiento borroso

    OpenAIRE

    Rafael Bello Pérez; Carlos Morell Pérez

    2011-01-01

    Se presenta una arquitectura para desarrollar de forma unificada tanto el razonamiento basado en reglas como el basado en casos considerando rasgos borrosos. El modelo permite usar varios de los procedimientos desarrollados para el razonamiento borroso basado en reglas en el razonamiento borroso basado en casos. Además, se proponen varias funciones de semejanza y funciones de comparación de rasgos para realizar la recuperación de casos con rasgos borrosos. Se muestra la aplicación del modelo ...

  1. Asistente para consultas espaciales en Hive

    OpenAIRE

    Maldonado Muñoz, José Emilio

    2015-01-01

    El trabajo descrito y desarrollado a lo largo de esta memoria tiene como objetivo principal establecer un esquema de decisiones básicas para realizar consultas sobre datos espaciales en Hadoop/Hive. Dicho esquema servirá de asistente y soporte para usuarios nuevos en Hive, que teniendo nociones mínimamente básicas sobre el lenguaje de consultas estándar SQL, quieran trabajar en un ámbito Big Data sobre datos espaciales. En definitiva, este trabajo sirve para facilitar y asistir la primera tom...

  2. Nuevos registros de clorofitas marinas para Cuba

    OpenAIRE

    A. Moreira; Fujii, M.T.; Suárez, A. M.; Wynne, M.J.

    2013-01-01

    A partir de muestras colectadas en la bahía de Cienfuegos y de Santa Lucía (Pinar del Río), se identificaron dos nuevos registros de macroalgas marinas (clorofitas) para Cuba: Cladophora blomquistii C. Hoek y Udotea fibrosa D. S. Littler & M. M. Littler. C. blomquistii se registra por primera vez para la región del Caribe ya que se había consignado solo para lagunas costeras de la Florida, y la parte noreste del golfo de México. Se ofrecen además comentarios sobre la taxonomía, ...

  3. pipetas usadas para I. A.

    Directory of Open Access Journals (Sweden)

    J. Castañeda

    2004-01-01

    Full Text Available La vagina y cerviz de las cerdas son estimuladas físicamente durante la inseminación. Este contacto puede afectar características reproductivas tales como el transporte espermático y el proceso de ovulación. En el presente trabajo se evaluaron desde el punto de vista económico y reproductivo, tres tipos de pipetas utilizadas para I. A. de cerdas de diferente material y forma. Se utilizaron 120 animales multíparos asignados de acuerdo a su orden de entrada al celo, en forma sucesiva a uno de tres grupos experimentales: 1 I. A. mediante pipeta reusable de hule tipo Melrose ( Medata, 2 I. A. con pipeta desechable de punta plástica en espiral y 3 I. A. con pipeta desechable con punta de poliestireno redonda (Medi chimica. No se encontró diferencia (P>0.05 entre los porcentajes de gestación, parición y promedio de lechones nacidos vivos por parto, en el tiempo de aplicación del semen, ni en el volumen del mismo, arrojado por las cerdas en los 10 min. posteriores a la I. A. Los valores promedio fueron de: 91.7%, 86.7%, 10.4 ± 2.8, 7.8 ± 2.6 min. y 10.4 ± 11.3 ml, respectivamente. El análisis de regresión, no mostró significancia en cuanto a paridad en ninguna de las variables estudiadas (P>0.05. Se concluye que las pipetas evaluadas no difieren en su efecto sobre el desarrollo reproductivo de la piara. Sin embargo, la pipeta reusable tipo Melrose ofrece ventaja económica bajo las condiciones del presente estudio

  4. Evaluación de patogenicidad de aislamientos nativos de hongos entomopatógenos sobre el gusano blanco de la papa, Premnotrypes vorax (Hustache Pathogenicity evaluation of native isolates of entomopathogenic fungí against andean weevil, Premnotrypes vorax (Hustache

    Directory of Open Access Journals (Sweden)

    Pinto Lucía

    2001-12-01

    Full Text Available

    El objetivo de este trabajo fue la evaluación de la capacidad patogénica de aislamientos nativos de los hongos entomopatógenos Beauveria bassiana y Metarhizium anisopliae, obtenidos de áreas naturales y de papa almacenada, sobre adultos del gusano blanco de la papa, Premnotrypes vorax (Hustache (COLEOPTERA: Curculionidae, insecto plaga de gran importancia en el cultivo de la papa en Colombia y otros países andinos. La evaluación fue realizada mediante bioensayos en condiciones de laboratorio, utilizando insectos adultos obtenidos en cría y capturados en campo. Se presentan datos sobre el establecimiento del pie de cría. Para la evaluación de la patogenicidad, se evaluó la mortalidad expresada por los aislamientos en relación con el tiempo, estimándose el tiempo letal cincuenta,TL50, y el tiempo letal noventa, TL90, y para dos de los aislamientos fue evaluada la mortalidad en relación con la concentración del inoculo, estimada mediante la concentración letal media, CL50. En todos los casos se presentaron porcentajes de mortalidad mayores del 45%. Se observaron diferencias en la mortalidad de los insectos dependiendo de su origen. De acuerdo con los resultados obtenidos el aislamiento de Beauveria bassiana 9770, aislado de larva de Premnotrypes vorax (TL50:4.7 días - 9.8 días; TL90: 14.1 días - 20.8 días; CL50:7.03 x 10Hypersurfaces with Isotropic Para-Blaschke Tensor

    Institute of Scientific and Technical Information of China (English)

    Jian Bo FANG; Kun ZHANG

    2014-01-01

    Let Mn be an n-dimensional submanifold without umbilical points in the (n+1)-dimen-sional unit sphere Sn+1. Four basic invariants of Mn under the Moebius transformation group of Sn+1 are a1-form Φ called moebius form, a symmetric (0, 2) tensor A called Blaschke tensor, a symmetric (0, 2) tensor B called Moebius second fundamental form and a positive definite (0, 2) tensor g called Moebius metric. A symmetric (0, 2) tensor D = A+μB called para-Blaschke tensor, where μ is constant, is also an Moebius invariant. We call the para-Blaschke tensor is isotropic if there exists a function λ such that D = λg. One of the basic questions in Moebius geometry is to classify the hypersurfaces with isotropic para-Blaschke tensor. When λ is not constant, all hypersurfaces with isotropic para-Blaschke tensor are explicitly expressed in this paper.

  5. Tableros para actividades plásticas

    OpenAIRE

    Alcante, Ariana; Debenedeto, Martín; Defeo, Emiliano; Truchi, Julieta; Wild, Diego

    2014-01-01

    Las características de este proyecto se desprenden de una necesidad real del Departamento de Plástica de la FBA: equipar algunas aulas para actividades plásticas con tableros o con atriles que puedan utilizarse en forma vertical, horizontal y, eventualmente, que sirvan para exponer trabajos. Además, tenían que tener un bajo costo de producción. (Párrafo extraído del texto a modo de resumen)

  6. Un almuerzo para llevar saludable (Packing Smart)

    Centers for Disease Control (CDC) Podcasts

    2011-08-22

    En este podcast para niños, los chicos de Kidtastics hablan sobre almuerzos creativos para llevarse a la escuela, que ayuden a mantenerse con energía el resto del día.  Created: 8/22/2011 by National Center for Emerging and Zoonotic Infectious Diseases (NCEZID).   Date Released: 1/3/2012.

  7. Kehidupan Sosial Para Homeless di Jepang

    OpenAIRE

    Handayani, Savitri

    2015-01-01

    Homuresu berasal dari bahasa Inggris yaitu homeless yang artinya tidak memiliki rumah. Tapi masyarakat Jepang dahulu menyebutnya furosha yang berarti gelandangan atau tunawisma. Pertumbuhan homeless pertama kali meningkat pada tahun 1960-an setelah perang dunia II. Homeless muncul akibat resesi ekonomi yang melanda Jepang. Para homeless tersebut berasal dari kaum buruh yoseba, para pekerja reguler dan non reguler. Yoseba adalah sebutan untuk tempat jasa bantuan untuk menunggu lowongan pekerja...

  8. Neurocisticercosis: Afiches para Capacitación

    Directory of Open Access Journals (Sweden)

    MARCELO ROJAS C

    2007-02-01

    Full Text Available En el sistema universitario (me atrevo a decir, de todo el mundo, es poco el contenido educativo relativo a la Transferencia tecnológica para efectos de la capacitación de la sociedad sobre problemas de salud (claros ejemplos desafiantes para los veterinarios son la hidatidosis y cisticercosis y otros; manejados en términos de cambios de conducta. Los términos de control y prevención están dados en un lenguaje académico, muy abstracto y obviamente intrascedente en la comunidad. Al punto que que las recomendaciones que manejan los Profesionales, resultan imprácticos en el ámbito social. Cada vez que voy a Provincias (interior del país y tengo la oportunidad de hablar sobre el control de las citadas metocestodiadis: mis auditorios quedan impactado y entendiendo la gravedad del Problema, y la tarea para solucionarlo. Los Técnicos Agropecuarios, los de las ONGs, los mismos Profesionales del Ministerio de Agricultura (SENASA, quedan impresionados del mensaje y contenido de los Afiches. Muchísimas veces me las han pedido (los afiches, pero les ofrecí publicarlos; porque considero que tiene gran creatividad y propiedad intelectual. He visto muchísimas imágenes de Capacitación; que en lugar de simplificar el mensaje, los complican. Es pues, una herramienta de trabajo para los capacitadores, para Veterinarios jóvenes e inexpertos, para la gente que está en contacto directo con el problema (pobladores; pero también lo será para tantos nuevos Profesores universitarios, sin experiencia de campo. Puede que impresione negativamente el lenguaje de los afiches; pero casualmente está hecho en el lenguaje popular que conoce la comunidad rural. La publicación persigue entregar a usarios capacitadores de una herramienta para publicitarlos masivamente.

  9. Fatores de risco para nascimento de crianças pequenas para idade gestacional

    OpenAIRE

    Zambonato Ana Maria Krusser; Pinheiro Ricardo Tavares; Horta Bernardo Lessa; Tomasi Elaine

    2004-01-01

    OBJETIVO: Determinar os fatores de risco para o nascimento de crianças pequenas para a idade gestacional. MÉTODOS: Nos meses de outubro a dezembro de 1996, cinco maternidades de Pelotas, RS, foram visitadas diariamente para entrevistar puérperas. O peso ao nascer foi obtido do registro dos berçários. A idade gestacional foi calculada a partir da data da última menstruação. Considerou-se como pequena para a idade gestacional (PIG) aquelas crianças cujo peso ao nascer, de acordo com o sexo e a ...

  10. Framework para implementar pruebas de usabilidad flexibles para aplicaciones móviles

    OpenAIRE

    Enriquez, Juan G.; Casas, Sandra

    2014-01-01

    Al realizar pruebas de usabilidad en aplicaciones móviles, dos de los factores a considerar son el contexto móvil de uso y la variabilidad del hardware de los dispositivos móviles. Este trabajo propone un framework para implementar pruebas de usabilidad que sean flexibles para los desarrolladores al encontrarse con diferente hardware, no intrusivas para la aplicación que se quiere probar y que recolecten, además de las métricas de usabilidad, información relacionada al contexto de uso. Para l...

  11. Factors affecting fungus-induced larval mortality in Anopheles gambiae and Anopheles stephensi

    Directory of Open Access Journals (Sweden)

    Takken Willem

    2010-01-01

    Full Text Available Abstract Background Entomopathogenic fungi have shown great potential for the control of adult malaria vectors. However, their ability to control aquatic stages of anopheline vectors remains largely unexplored. Therefore, how larval characteristics (Anopheles species, age and larval density, fungus (species and concentration and environmental effects (exposure duration and food availability influence larval mortality caused by fungus, was studied. Methods Laboratory bioassays were performed on the larval stages of Anopheles gambiae and Anopheles stephensi with spores of two fungus species, Metarhizium anisopliae and Beauveria bassiana. For various larval and fungal characteristics and environmental effects the time to death was determined and survival curves established. These curves were compared by Kaplan Meier and Cox regression analyses. Results Beauveria bassiana and Metarhizium anisopliae caused high mortality of An. gambiae and An. stephensi larvae. However, Beauveria bassiana was less effective (Hazard ratio (HR Metarhizium anisopliae. Anopheles stephensi and An. gambiae were equally susceptible to each fungus. Older larvae were less likely to die than young larvae (HR Conclusions This study shows that both fungus species have potential to kill mosquitoes in the larval stage, and that mortality rate depends on fungus species itself, larval stage targeted, larval density and amount of nutrients available to the larvae. Increasing the concentration of fungal spores or reducing the exposure time to spores did not show a proportional increase and decrease in mortality rate, respectively, because the spores clumped together. As a result spores did not provide uniform coverage over space and time. It is, therefore, necessary to develop a formulation that allows the spores to spread over the water surface. Apart from formulation appropriate delivery methods are also necessary to avoid exposing non-target organisms to fungus.

  12. UM TRATADO PARA O AMOR

    Directory of Open Access Journals (Sweden)

    Maria José Caldeira do Amaral

    2015-07-01

    Full Text Available No Tratado do Amor Cortês - Tractatus de Amore - André Capelão deseja oferecer ao leitor a arte cortês de amar, a beleza do desejo erótico e a disciplina da paixão constitutiva dessa arte que é amar. O maior argumento dessa obra é efetivar o suspiro fundo que a dimensão amorosa infunde na alma do homem e da mulher aos quais foi concedida a virtude. Para esse autor, escriba da chancelaria real e talvez, não se sabe com certeza, um Ordo Sacratus, o amor é a raiz de toda cortesia, a fonte de todo bem. Nosso objetivo, então, é encontrar nessa suma amorosa construída nos enlaces da Literatura de Cavalaria Medieval, os desdobramentos essenciais dessa vida afetiva bela e virtuosa no enredo profundo do qual emerge o afeto, a fé e a contradição da experiência humana do sofrimento amoroso. Palavras Chave: Literatura Medieval. Amor Cortês, André Capelão, virtude, desejo. Abstract: With the treaty toward the love - Tractatus de Amore – André Capelão offers to the reader the courteous’ art for love, the erotic desire’s beauty and the  suffering discipline belong this love’s art  is. The best reason of this literary composition is to achieve the depth sigh that the loving dimension infuses into the lovers’s soul, the man and the woman wich this virtue was conceded. For this author, the royal chancery scribe and perhaps, no one knows for sure, a Sacratus Ordo, love is the root of all free, the source of all good. Our goal, then, is to find this sum loving built in interlacing of Medieval Knights literature, the essential developments of this beautiful and virtuous love life in the deep storyline which emerges affection, faith and the contradiction of the human experience of love suffering. Key words: Medieval Literature. Courteous Love. André Capelão. Virtue.

  13. Compatibility among entomopathogenic hyphocreales and two beneficial insects used to control Trialeurodes vaporariorum (Hemiptera: Aleurodidae) in Mediterranean greenhouses.

    Science.gov (United States)

    Hamdi, Faten; Fargues, Jacques; Ridray, Gilles; Jeannequin, Benoît; Bonato, Olivier

    2011-09-01

    The effect of the combined use of Encarsia formosa or Macrolophus caliginosus and one of three marketed mycoinsecticides, Mycotal® (Leucanicillium muscarium-based), Naturalis-L™ (Beauveria bassiana-based) and PreFeRal® (Isaria fumosorosea-based), on the control of the whitefly, Trialeurodes vaporariorum, was studied under laboratory and greenhouse conditions. The results of both types of tests, the bioassays and the greenhouse trials, for all combinations of E. Formosa with each of the three mycoinsecticides showed that the total mortality of larval populations of T. vaporariorum was not affected. The mortality of T. vaporariorum larvae treated in the second instar revealed the capacity for both B. bassiana- and L. muscarium-based formulations and E. formosa to kill the host either separately or in association. Because of its higher pathogenic activity (under our test conditions), L. muscarium provoked a large proportion of mycoses in larvae exposed to parasitization. In contrast, the efficacy of parasitization was higher in larvae treated with B. bassiana and exposed to E. formosa because of a lower pathogenic activity of the fungus. Bioassays carried out with third-instar larvae of T. vaporariorum showed a low susceptibility to both tested fungi. Consequently, mortalities recorded in larvae subjected to the combined treatments by consecutive exposures or at 2-4 days post-parasitization were mainly caused by the development of the parasitoid. Greenhouse trials showed that fungus-induced mortality of T. vaporariorum in plants treated with L. muscarium, I. fumosorosea, and B. Bassiana was significant compare to control. L. muscarium, B. bassiana and I. fumosorosea killed young whitefly larvae and limited parasitization to 10% or less. Second-instar larvae of M. caliginosus were not susceptible to L. muscarium and B. bassiana formulations with any mode of contamination: direct spraying of larvae, spraying on the foliar substrate or by contaminated T. vaporariorum

  14. High Ozone (O3) Affects the Fitness Associated with the Microbial Composition and Abundance of Q Biotype Bemisia tabaci

    Science.gov (United States)

    Hong, Yanyun; Yi, Tuyong; Tan, Xiaoling; Zhao, Zihua; Ge, Feng

    2016-01-01

    Ozone (O3) affects the fitness of an insect, such as its development, reproduction and protection against fungal pathogens, but the mechanism by which it does so remains unclear. Here, we compared the fitness (i.e., the growth and development time, reproduction and protection against Beauveria bassiana (B. bassiana) of Q biotype whiteflies fumigated under hO3 (280 ± 20 ppb) and control O3 (50 ± 10 ppb) concentrations. Moreover, we determined that gene expression was related to development, reproduction and immunity to B. bassiana and examined the abundance and composition of bacteria and fungi inside of the body and on the surface of the Q biotype whitefly. We observed a significantly enhanced number of eggs that were laid by a female, shortened developmental time, prolonged adult lifespan, decreased weight of one eclosion, and reduced immunity to B. bassiana in whiteflies under hO3, but hO3 did not significantly affect the expression of genes related to development, reproduction and immunity. However, hO3 obviously changed the composition of the bacterial communities inside of the body and on the surface of the whiteflies, significantly reducing Rickettsia and enhancing Candidatus_Cardinium. Similarly, hO3 significantly enhanced Thysanophora penicillioides from the Trichocomaceae family and reduced Dothideomycetes (at the class level) inside of the body. Furthermore, positive correlations were found between the abundance of Candidatus_Cardinium and the female whitefly ratio and the fecundity of a single female, and positive correlations were found between the abundance of Rickettsia and the weight of adult whiteflies just after eclosion and immunity to B. bassiana. We conclude that hO3 enhances whitefly development and reproduction but impairs immunity to B. bassiana, and our results also suggest that the changes to the microbial environments inside of the body and on the surface could be crucial factors that alter whitefly fitness under hO3.

  15. Sequential fungal fermentation-biotransformation process to produce a red pigment from sclerotiorin.

    Science.gov (United States)

    Corrêia Gomes, Dhionne; Takahashi, Jacqueline Aparecida

    2016-11-01

    The fungus Penicillium sclerotiorum produces sclerotiorin, an orange compound closely related to the useful food coloring pigments produced by Monascus species. The high productivity, together with several biological activities reported for sclerotiorin highlights its potential application in food industry. In this work, sclerotiorin was obtained as the major metabolite produced in liquid fermentation by P. sclerotiorum standing for 30% of the fungal dry extract. Modulation of sclerotiorin color was accomplished by biotransformation using Beauveria bassiana generating a red derivative with 13.8% yield. Color modification was caused by fungal-mediated substitution of oxygen by nitrogen in the pyrone ring changing the molecule's chromophore. A derivative, 1-methyl sclerotiorin was synthesized from sclerotiorin using diazomethane and fed to B. bassiana. In this case, substituent at C-1 avoided heteroatom substitution. Sclerotiorin derivatives obtained in the present show the great potential of sclerotiorin derivatives as food colorants. PMID:27211658

  16. Alimentos balanceados para perros en Costa Rica

    Directory of Open Access Journals (Sweden)

    Ruth Vargas

    2001-01-01

    Full Text Available Diagnóstico de la comercialización de alimentos balanceados para perros en Costa Rica. Para ampliar los reportes oficiales de la comercialización de alimentos para perros se creó una base de datos que incluyó cantidad, costo, empaque y formulación durante el período 1998 a agosto del 2000. De 1995 a 1998 y de 1996 a 1999 la producción nacional incrementó un 90,4% y la importación un 42,56% respectivamente, ocupando los alimentos nacionales un 72% del tonelaje y un 70% del valor en dólares americanos. No fue posible determinar cuál es el empaque más comercializado, pero si que los alimentos extrusados son los que ocupan el primer lugar y que los alimentos recomendados para cachorros y adultos son los que más se comercializan. La ausencia de datos en las declaraciones sugiere la necesidad de incrementar el control en las mismas en caso de requerirse un estudio de mercado, ya que contrariamente a esta situación el mayor número de garantías inscritas corresponden separadamente para las fases de cachorros y adultos. Merece también atención que las garantías indican mayores porcentajes de proteína cruda que los recomendados por la AAFCO y que al menos 36 fórmulas son recomendadas para estados sanitarios específicos (p.e. para perros con problemas de alergias, cálculos renales, pérdidas de pelo, etc. sin que oficialmente se encuentre registrada ninguna fórmula medicada

  17. Can insects develop resistance to insect pathogenic fungi?

    Directory of Open Access Journals (Sweden)

    Ivan M Dubovskiy

    Full Text Available Microevolutionary adaptations and mechanisms of fungal pathogen resistance were explored in a melanic population of the Greater wax moth, Galleria mellonella. Under constant selective pressure from the insect pathogenic fungus Beauveria bassiana, 25(th generation larvae exhibited significantly enhanced resistance, which was specific to this pathogen and not to another insect pathogenic fungus, Metarhizium anisopliae. Defense and stress management strategies of selected (resistant and non-selected (susceptible insect lines were compared to uncover mechanisms underpinning resistance, and the possible cost of those survival strategies. We hypothesize that the insects developed a transgenerationally primed resistance to the fungus B. bassiana, a costly trait that was achieved not by compromising life-history traits but rather by prioritizing and re-allocating pathogen-species-specific augmentations to integumental front-line defenses that are most likely to be encountered by invading fungi. Specifically during B. bassiana infection, systemic immune defenses are suppressed in favour of a more limited but targeted repertoire of enhanced responses in the cuticle and epidermis of the integument (e.g. expression of the fungal enzyme inhibitor IMPI, and cuticular phenoloxidase activity. A range of putative stress-management factors (e.g. antioxidants is also activated during the specific response of selected insects to B. bassiana but not M. anisopliae. This too occurs primarily in the integument, and probably contributes to antifungal defense and/or helps ameliorate the damage inflicted by the fungus or the host's own immune responses.

  18. Effects of entomopathogenic fungi on different developmental stages of Cotesia flavipes (Cam. a parasitoid of Diatraea flavipennella (Box (Lepidoptera: Crambidae

    Directory of Open Access Journals (Sweden)

    Cinthia Conceição Matias da Silva

    2016-02-01

    Full Text Available Biological control using the parasitoid Cotesia flavipes (Cam. is one of the main components in the integrated management of the sugarcane moth borer Diatraea spp. Besides this parasitoid, the entomopathogenic fungi Beauveria bassiana (Bals. Vuill and Metarhizium anisopliae (Metsch. Sorok. are used to control sugarcane pests, and they can be found naturally parasitizing caterpillars. This study aimed to evaluate the effects of M. anisopliae and B. bassiana on different developmental stages of the C. flavipes parasitoid on Diatraea flavipennella (Box. The experiments were carried at laboratory with isolates PL 43 of M. anisopliae and ESALQ 447 of B. bassiana were applied to the parasitoid at the immature and adult phases. No negative effects were observed on the larval development of C. flavipes, and it could complete its development on caterpillars of D. flavipennella treated with fungi. The fungi did not cause pupal mortality. However, B. bassiana caused high mortality in the adult parasitoid (76%. The fungi had negative effects on parasitoids when applied during certain developmental stages of C. flavipes.

  19. A proposed role for the cuticular fatty amides of Liposcelis bostrychophila (Psocoptera: Liposcelidae) in preventing adhesion of entomopathogenic fungi with dry-conidia.

    Science.gov (United States)

    Lord, Jeffrey C; Howard, Ralph W

    2004-08-01

    Maximum challenge exposure of Liposcelis bostrychophila to Beauveria bassiana, Paecilomyces fumosoroseus, Aspergillus parasiticus or Metarhizium anisopliae resulted in no more than 16% mortality. We investigated several of L. bostrychophila's cuticular lipids for possible contributions to its tolerance for entomopathogenic fungi. Saturated C14 and C16 fatty acids did not reduce the germination rates of B. bassiana or M. anisopliae conidia. Saturated C6 to C12 fatty acids that have not been identified in L. bostrychophila cuticular extracts significantly reduced germination, but the reduction was mitigated by the presence of stearamide. Cis-6-hexadecenal did not affect germination rates. Mycelial growth of either fungal species did not occur in the presence of caprylic acid, was reduced by the presence of lauric acid, and was not significantly affected by palmitic acid. Liposcelis bostrychophila is the only insect for which fatty acid amides have been identified as cuticular components. Stearamide, its major fatty amide, did not reduce germination of B. bassiana or M. anisopliae conidia or growth of their mycelia. Adhesion of conidia to stearamide preparations did not differ significantly from adhesion to the cuticle of L. bostrychophila. Pretreatment of a beetle known to be fungus-susceptible, larval Oryzaephilus surinamensis, with stearamide significantly decreased adhesion of B. bassiana or M. anisopliae conidia to their cuticles. This evidence indicates that cuticular fatty amides may contribute to L. bostrychophila's tolerance for entomopathogenic fungi by decreasing hydrophobicity and static charge, thereby reducing conidial adhesion.

  1. Comparative performance evaluation of multi-metal resistant fungal strains for simultaneous removal of multiple hazardous metals.

    Science.gov (United States)

    Dey, Priyadarshini; Gola, Deepak; Mishra, Abhishek; Malik, Anushree; Kumar, Peeyush; Singh, Dileep Kumar; Patel, Neelam; von Bergen, Martin; Jehmlich, Nico

    2016-11-15

    In the present study, five fungal strains viz., Aspergillus terreus AML02, Paecilomyces fumosoroseus 4099, Beauveria bassiana 4580, Aspergillus terreus PD-17, Aspergillus fumigatus PD-18, were screened for simultaneous multimetal removal. Highest metal tolerance index for each individual metal viz., Cd, Cr, Cu, Ni, Pb and Zn (500mg/L) was recorded for A. fumigatus for the metals (Cd, 0.72; Cu, 0.72; Pb, 1.02; Zn, 0.94) followed by B. bassiana for the metals (Cd, 0.56; Cu, 0.14; Ni, 0.29; Zn, 0.85). Next, the strains were exposed to multiple metal mixture (Cd, Cr, Cu, Ni, Pb and Zn) of various concentrations (6, 12, 18, 30mg/L). Compared to other strains, B. bassiana and A. fumigatus had higher cube root growth (k) constants indicating their better adaptability to multi metal stress. After 72h, multimetal accumulation potential of B. bassiana (26.94±0.07mg/L) and A. fumigatus (27.59±0.09mg/L) were higher than the other strains at initial multimetal concentration of 30mg/L. However, considering the post treatment concentrations of individual metals in multimetal mixture (at all the tested concentrations), A. fumigatus demonstrated exceptional performance and could bring down the concentrations of Cd, Cu, Ni, Pb and Zn below the threshold level for irrigation prescribed by Food and Agriculture Organization (FAO). PMID:27497228

  2. Biochemiluminescence of certain fungi

    Directory of Open Access Journals (Sweden)

    Janusz Sławiński

    2014-11-01

    Full Text Available Twelve species of fungi growing on the Sabouraud medium in darkness and illumination in an incubator, were tested to find out their ability to emit the ultra-weak biochemiluminescence. Using a sensitive photon-counling device, it was possible to measure biochemiluminescence intensity during ten days of cultures growth. Boletus edulis, Pestalotia funerea and Microsporum gypseum displayed biochemiluminescence, while Aspergillus nidulans, A. quadrilineatus, Beauveria bassiana, Macrophoma candollei, Mucor lausanensis, Paecilomyces farinosus, Penicillium sp., Trichoderma lignorum and Tricholoma equestre failed to do it. Illumination put down biochemiluminescence and stimulated colour formation in both mycelia and in the medium.

  3. 一种昆虫病原真菌寄生菌的调查和鉴定

    Institute of Scientific and Technical Information of China (English)

    武觐文

    1987-01-01

    寄生在白僵菌(Beauveria bassiana),粉质拟青霉(Paecilomyces farinosus)等昆虫病原真菌上的寄生黑孢壳(Melanospora parasitica)在世界上早有报导,并分布在很多国家,在我国尚无人报导,本文介绍北京地区见到的该菌的形态,鉴定,分离方法以及野外的调查情况。

  4. Stimulation of Armillaria rhizomorph growth by oak root fungi

    Directory of Open Access Journals (Sweden)

    Hanna Kwaśna

    2014-08-01

    Full Text Available Thirty one different genera of fungi were isolated from the wood of roots of 5O·year·old oak (Quercus robur. The most frequently isolated fungi were: Mycelium radicis atrovirens alpha (MRAA, Clonostachys sp. and Penicillium daleae, Beauveria bassiana, Clonostachys sp., Cryplosporiopsis rodicicolo, Geotrichum candidum, Mortierella vinacea, MRAA, P. daleae, P. janczewskii P. spinulosum, Sporothrix schenckii and Tolypocladium niveum significantly enhanced Armillaria mellea rhizomorph initiation and growth from oak branch segments in vitro. The biggest stimulation effect was noticed when the dematiaceous hyphomycetes, e.g. MRAA, P. dimorphospora and S. schenckii were studied.

  5. Spatial and taxonomical overlap of fungi on phylloplanes and invasive alien ladybirds with fungal infections in tree crowns of urban green spaces.

    Science.gov (United States)

    Howe, Andy G; Ravn, Hans Peter; Jensen, Annette B; Meyling, Nicolai V

    2016-09-01

    Occurrence of entomopathogenic fungi on phylloplanes in Tilia × europaea crowns between 1 and 13 m was assessed in urban parks. Prevalence of fungal infections in ladybirds (Coleoptera: Coccinellidae) collected from Tilia × europaea was assessed to determine whether fungi found on phylloplanes also occurred as infections in ladybirds. Isaria spp. was most abundant on phylloplanes (mean colony forming units (CFU) per leaf ± SE, 0.33 ± 0.03) followed by Beauveria spp. (0.22 ± 0.02 CFU per leaf) and Lecanicillium spp. (0.19 ± 0.02 CFU per leaf). Densities of inoculum were higher in inner crowns and decreased with height, although Lecanicillium spp. peaked at 5-7 m. Upper phylloplane surfaces harboured higher densities of Isaria spp. and Beauveria spp. than lower surfaces, whereas Lecanicillium spp. was equally distributed. Most prevalent on ladybirds were Isaria spp. (20.6% Harmonia axyridis; 4.8% natives), Lecanicillium spp. (13.6% H. axyridis; 4.8% natives), with fewer Beauveria spp. infections (2.6% H. axyridis). Molecular identification revealed Beauveria bassiana, B. pseudobassiana, Isaria farinosa and Lecanicillium muscarium among isolates of both tree and ladybird origin. Tilia × europaea phylloplanes support a diverse assemblage of entomopathogenic fungal species with a different prevalence in coccinellids compared to their relative abundance in this habitat. PMID:27353660

  6. Sensibilidade de fungos entomopatogênicos a agroquímicos usados no manejo da cana-de-açúcar

    Directory of Open Access Journals (Sweden)

    Aline Aparecida Alves Botelho

    2011-01-01

    Full Text Available Os agroquímicos empregados no manejo da cana-de-açúcar podem afetar a ação de fungos entomopatogênicos usados no controle biológico de pragas da cultura. Este trabalho teve por objetivo investigar se os inseticidas, herbicidas e maturadores utilizados no manejo da cana-de-açúcar têm efeito tóxico sobre os fungos Beauveria bassiana e Metarhizium anisopliae. Foram utilizados os inseticidas thiametoxan, aldicarbe e fipronil, os herbicidas imazapir, diuron, metribuzin, hexazinone+diuron, clomazone+ametrina, 2,4 diclorofenoxiacético e glifosato, e os maturadores etil-trinexapac, sulfometurom-metílico e glifosato também. Os fungos foram cultivados em meio de cultura batata-dextrose-ágar contendo os agroquímicos. Avaliou-se o crescimento micelial, a produção e viabilidade dos conídios, e fez-se a avaliação da toxicidade dos agroquímicos. O inseticida à base de thiametoxan foi considerado compatível, pois não afetou o crescimento micelial, a produção e a viabilidade dos conídios dos dois fungos. O inseticida formulado com fipronil se mostrou parcialmente tóxico para os fungos, sendo considerado moderadamente compatível, enquanto o aldicarbe foi considerado tóxico. Os herbicidas avaliados afetaram o crescimento micelial, a produção e a viabilidade dos conídios dos entomopatógenos e foram classificados como tóxicos, mas aqueles formulados com imazapir, glifosato e metribuzim foram considerados compatíveis. Entre os agroquímicos usados como maturadores apenas o glifosato foi classificado como compatível. Os agroquímicos usados no manejo da cana-de-açúcar, e que foram testados neste estudo, têm majoritariamente efeito tóxico sobre B. bassiana e M. anisopliae podendo comprometer sua ação como bioagentes de controle de pragas da cultura.

  7. Para-product operators and para-linearization on locally compact Vilenkin groups

    Institute of Scientific and Technical Information of China (English)

    苏维宜

    1995-01-01

    The concept of para-product operators over locally compact Vilenkin groups is established and the applications to the para-linearization in nonlinear problems are studied. This kind of operators plays a special role in dealing with those functions which do not have the classical derivatives.

  8. LITERATURA PARA JOVENS LEITORES: UMA PROPOSTA

    Directory of Open Access Journals (Sweden)

    Gizelle Kaminski Corso

    2011-08-01

    Full Text Available Percebendo que o público-alvo das adaptações de textos clássicos não é tão somente a criança e o adolescente – leitores jovens, aos quais supostamente destinam-se as literaturas “infantil” e “infantojuvenil”, proponho neste texto uma nova classificação para as adaptações literárias, vendo-as não mais apenas como “adaptações para a literatura infantojuvenil”, mas como “literatura para jovens leitores”. Como tais textos são também lidos por adultos, por propósitos diversos, reconheço que o conceito proposto abrange um número maior de leitores. Para tanto, faço um breve percurso histórico-crítico acerca do aparecimento das adaptações no cenário literário brasileiro com a finalidade de corroborar e justificar a pertinência da classificação sugerida para as adaptações.

  9. Instrumento para o gerenciamento de riscos ambientais

    Directory of Open Access Journals (Sweden)

    Thaiza Clemente Couto Bissacot

    2016-01-01

    Full Text Available RESUMO: Embora existam normas e metodologias padronizadas para a análise de riscos ambientais dentro de uma organização, os mecanismos e/ou instrumentos disponíveis estão sujeitos à subjetividade, não são automatizados, não estabelecem critérios técnicos e, de maneira geral, não apresentam mecanismos de priorização para a tomada de decisão. Com base nas lacunas observadas, a presente nota técnica apresenta as premissas de um Instrumento de Gestão para a Identificação e o Gerenciamento de Riscos Ambientais (IGIGRA. O dashboard consolidado pelo IGIGRA permitirá a visualização consolidada dos riscos ambientais do empreendimento, proporcionando comparações entre diferentes operações e períodos de avaliação, fornecendo base técnica para a priorização de ações e alocação de recursos para o controle dos riscos dentro da organização.

  10. La importancia de FOCALAE para Colombia

    Directory of Open Access Journals (Sweden)

    Pío García

    2011-11-01

    Full Text Available Las asociaciones regionales están condicionadas por la dinámica del poder global; no obstante, desempeñan un papel decisivo para afrontar el reto de establecer un orden mundial convenido y equitativo. El mecanismo idóneo de la relación transpacífica latinoamericana es FOCALAE, un foro de cooperación múltiple extendida al ámbito económico, social, cultural, educativo y deportivo. Los resultados de su primera década de existencia fueron magros, en razón, entre otros motivos, de la ausencia de proyectos atractivos para los países asiáticos y para América Latina, y de la fractura ideológica profunda que ha soportado esta última. Con el potencial inocultable para contribuir al bienestar de Asia Pacífico y de Latinoamérica por medio del intercambio extenso, el Foro tendrá que esperar tiempos más favorables para su despliegue, cuando ciertos liderazgos regionales, en los que el colombiano ha de estar presente, vuelvan a impulsar la concertación latinoamericana.

  11. Modelo para el análisis de una aptitud cognitiva para el aprendizaje

    Directory of Open Access Journals (Sweden)

    Clara Cecilia Uribe Hernández

    2009-01-01

    Full Text Available La investigación en el campo de las aptitudes cognitivas para el aprendizaje requiere una forma de abordarlas y definir sus características y componentes. A su vez, esta definición debe conducir al investigador a la creación de pruebas de medición que ayuden al análisis de este tipo de aptitudes y su relación con otras variables del aprendizaje. Para dar respuesta a esas necesidades, el objetivo de este artículo es proponer la creación de un modelo para el análisis de una aptitud cognitiva para el aprendizaje, cuya estructura conste de cuatro elementos principales. Dos de ellos relacionados con los enfoques y consideraciones para emprender su estudio; los otros dos, con la definición de la aptitud, a través de sus características y sus componentes.

  12. Los videojuegos: conectar alumnos para aprender

    Directory of Open Access Journals (Sweden)

    José Antonio Valderrama Ramos

    2012-01-01

    Full Text Available Este artículo explora la utilidad que presentan los videojuegos comerciales actuales para educar a los jugadores y ayudarlos a adquirir habilidades necesarias para desempeñarse mejor en nuestra sociedad. Aborda el problema de la educación formal, la cual no está respondiendo a la situación de complejidad de nuestro entorno y usa el paradigma del pensamiento complejo y siete saberes para la educación propuestas por Edgar Morin. También, describe algunas ideas que le pueden ser de utilidad a docentes interesados en usar videojuegos como herramientas pedagógicas en el salón de clases.

  13. Rutina AUTOLISP para el despiece de columnas

    Directory of Open Access Journals (Sweden)

    Lorena Cocunubo Carreño

    2014-06-01

    Full Text Available El proyecto de creación de una herramienta Autolisp para el dibujo automático de los despieces de vigas sometidas a flexión y a cortante se desarrolla en un ambiente de programación informática LISP. Se inicia con la programación en el Visual Lisp de AutoCAD del algoritmo que permite tomar información de anchos de luz, sección transversal y momentos resistentes la viga para que automáticamente ejecute los comandos y el resultado final sea un despiece detallado de los hierros por flexión junto con los estribos por cortante separados adecuadamente. Los resultados están sujetos a criterios de diseños de la NSR-10 para así garantizar la seguridad del diseño sísmico.

  14. Estrategias de mercado para el frijol centroamericano

    Directory of Open Access Journals (Sweden)

    Lourdes Mart\\u00EDnez

    2004-01-01

    Full Text Available Estrategias de mercado para el frijol centroamericano. En los últimos años, el sub-sector de frijol en Centro América ha sido testigo de varios cambios dinámicos. Si no se encuentran medidas para incrementar la competitividad regional de este sub-sector, los países centroamericanos experimentarán impactos negativos en las áreas sociales y económicas de considerable magnitud, especialmente ahora que Centro América necesita ajustar su economía a los nuevos requerimientos de mercados mas abiertos, como el Tratado Centroamericano de Libre Comercio con USA (TLC. Vendedores de frijoles a nivel intermediario y de consumo final, así como técnicos de oficinas gubernamentales dedicadas al mercadeo de frijoles en Centro América fueron entrevistados para documentar los cambios experimentados, identificar los obstáculos que frenan la viabilidad del sub-sector e identificar las oportunidades de incremento de mercados en el futuro para los productores y vendedores de frijol. Los resultados muestran que para que continúen siendo competitivos, los países centroamericanos deben incrementar la productividad a través del apoyo a las investigaciones para el mejoramiento de variedades de frijol, especialmente aquellas que los consumidores prefieren por características específicas (e.g. color. También es imprescindible el fortalecimiento de los programas de distribución de variedades mejoradas a los productores; las mejoras en la transmisión de información de mercado a los productores; el fortalecimiento de los contactos entre productores, comercializadores a nivel mayorista y supermercados; y una política más específica de apertura de mercados de exportación, especialmente con los Estados Unidos.

  15. Oxitocina: nuevas perspectivas para una droga antigua

    Directory of Open Access Journals (Sweden)

    Steven L. CLARK

    2010-12-01

    Full Text Available La oxitocina es el medicamento más comúnmente asociada con la prevención de complicaciones perinatales y recientemente fue incluida por el Instituto para la Administración Medicamentosa Segura (Institute for Safe Medication Practices - ISMP en una pequeña lista de medicamentos “con alto grado de riesgos”, lo que puede “necesitar salvaguardas especiales para reducir el riesgo de error”. Las recomendaciones actuales para administrar este medicamento son vagas en relación a la indicación, tiempo de uso, dosis y el monitoreo de los efectos maternos y fetales. Una revisión de los datos clínicos y farmacológicos disponibles sugiere que las orientaciones específicas y basadas en evidencias para administrar oxitocina durante el parto puede derivarse de datos disponibles. Si implementadas, estas prácticas pueden reducir la probabilidad de daños al paciente. Esto sugiere el foco de las orientaciones para la administración electiva limitada de oxitocina, considerar estrategias que disminuyan la necesidad de usar oxitocina, confiar en terapéuticas de bajas dosis de oxitocina, la adhesión a definiciones semicuantitativas específicas sobre parto adecuado e inadecuado, y la aceptación de que, una vez que la actividad uterina adecuada sea alcanzada, es generalmente preferible esperar más tiempo que aumentar la infusión de oxitocina. El uso de protocolos conservadores y específicos para monitorear los efectos de la oxitocina en la madre y en el bebé tiende no sólo a aumentar los resultados, mas también a reducir los conflictos entre miembros del equipo obstétrico. Implementar estas orientaciones parece ser apropiada en una cultura cada vez más focalizada en la seguridad del paciente.

  16. Controlador DNP3 para la CIAA

    OpenAIRE

    Tobar, Sebastián; Noguera, Joel; Taffernaberry, Juan Carlos; Mercado, Gustavo

    2016-01-01

    La CIAA [1] (Computadora Industrial Abierta Argentina) tiene las características necesarias para utilizarse como RTU (Remote Terminal Unit - Unidad Terminal Remota) en sistemas SCADA [2] (Supervisory Control And Data Acquisition – Supervisión Control y Adquisición de Datos). Sin embargo, una gran limitación para esta aplicación radica en que actualmente soporta un único protocolo de comunicación: Modbus. Si bien este protocolo es, probablemente, el más utilizado históricamente en la industria...

  17. Un modelo SETAR para el PIB colombiano

    OpenAIRE

    Vivas Lorena; Ramos Johanna; Hoyos Milena

    2010-01-01

    En este artículo se estudia el comportamiento de la tasa de crecimiento del PIB colombiano durante el período 1982-2008 a partir de un modelo SETAR (Self-Exciting Threshold Autoregressive), con base en la metodología propuesta por Tsay (1989) y Tong (1990) para la detección de no linealidades relacionadas con la existencia de regímenes cambiantes. Adicionalmente, se evalúa el desempeño de los pronósticos generados en relación a los obtenidos con un modelo autorregresivos lineal para diferente...

  18. Espacio urbano para el desarrollo sostenible

    Directory of Open Access Journals (Sweden)

    Guillermo Malca Obregoso

    2004-01-01

    Full Text Available Este artículo ilustra una Metodología de Análisis-Propuesta Urbana Sostenible para la enseñanza-aprendizaje del diseño urbano-arquitectónico conciente de su entorno socio-cultural-ambiental. Se presenta como alternativa a los métodos convencionales de intervención urbana. Pretende, a partir de la visión dialéctica, entender los problemas originados por el crecimiento de las ciudades de modo INTEGRAL, sin segmentarlos para su estudio parcializado y parcelado.

  19. "mbigoo!", la red social para foodies.

    OpenAIRE

    García Pumar, Javier

    2016-01-01

    Este trabajo fin de grado consiste en la elaboración de la web de una red social para aficionados a la gastronomía, englobando todo el ciclo de procesos de creación y siguiendo los principios del diseño centrado en el usuario (DCU), para obtener un primer prototipo con unas funcionalidades mínimas, que sea flexible y ampliable en el futuro, y que represente una opción de negocio. A partir de analizar las tendencias actuales, surge la gastronomía como un campo en plena ebullición, ...

  20. Coherencia curricular y oportunidades para aprender Ciencias

    OpenAIRE

    Marcela Ruiz Zúñiga; Alejandra Meneses Arévalo; Maximiliano Montenegro Maggio

    2014-01-01

    Se determinó la coherencia curricular entre oportunidades de aprendizaje y habilidades científicas ofrecidas por un texto escolar chileno y por las actividades de aula en 5º grado asociadas al contenido "Fuerza". Se realizó un análisis discursivo de la unidad didáctica del texto y de las actividades con el fin de construir matrices de contenido para tópicos y habilidades; la medición de coherencia curricular se realizó a través del índice de Porter para la coherencia. Los resultados muestran ...

  1. Valores de referência para carboxiemoglobina

    Directory of Open Access Journals (Sweden)

    Siqueira Maria Elisa P. B. de

    1997-01-01

    Full Text Available INTRODUÇÃO: Os valores de referência de indicadores biológicos são utilizados como parâmetros para interpretação de resultados de valores obtidos em indivíduos expostos ocupacionalmente aos agentes químicos. O Grupo Brasileiro para Estabelecimento dos Valores de Referência tem se dedicado a estas determinações objetivando estabelecer valores de referência para os diferentes bioindicadores em diversas regiões do País. Determinaram-se os valores de referência para a carboxiemoglobina (COHb no Sul de Minas Gerais. MATERIAL E MÉTODO: A COHb foi analisada pelo método espectrofométrico, otimizado no laboratório de análises toxicológicas. Em todas as amostras também foram realizadas análises de alguns parâmetros bioquímicos e hematológicos para atestar o estado de saúde da população, constituída de 200 voluntários não-fumantes e não-expostos, por motivo profissional, ao monóxido de carbono. Cada indivíduo respondeu um questionário para levantamento de dados relevantes à interpretação dos resultados. Os valores de referência foram expressos em termos da média ± desvio-padrão, intervalo de confiança 95% e valor de referência superior. A distribuição estatística dos resultados obtidos foi realizada para possibilitar sua comparação com grupos de trabalhadores, preferentemente à avaliação individual. RESULTADOS E CONCLUSÕES: O valor médio ± desvio-padrão para a carboxiemoglobina foi de 1,0 % ± 0,75; o intervalo de confiança 95%, entre 0,9 e 1,1 % e o valor de referência superior, de 2,5%. Através do teste t de Student (p < 0,05 não foi detectada diferença nos valores de acordo com o sexo, idade ou uso de bebidas alcoólicas. Os valores de referência encontrados foram similares aos reportados em outros países.

  2. Valores de referência para carboxiemoglobina

    Directory of Open Access Journals (Sweden)

    Maria Elisa P. B. de Siqueira

    1997-12-01

    Full Text Available INTRODUÇÃO: Os valores de referência de indicadores biológicos são utilizados como parâmetros para interpretação de resultados de valores obtidos em indivíduos expostos ocupacionalmente aos agentes químicos. O Grupo Brasileiro para Estabelecimento dos Valores de Referência tem se dedicado a estas determinações objetivando estabelecer valores de referência para os diferentes bioindicadores em diversas regiões do País. Determinaram-se os valores de referência para a carboxiemoglobina (COHb no Sul de Minas Gerais. MATERIAL E MÉTODO: A COHb foi analisada pelo método espectrofométrico, otimizado no laboratório de análises toxicológicas. Em todas as amostras também foram realizadas análises de alguns parâmetros bioquímicos e hematológicos para atestar o estado de saúde da população, constituída de 200 voluntários não-fumantes e não-expostos, por motivo profissional, ao monóxido de carbono. Cada indivíduo respondeu um questionário para levantamento de dados relevantes à interpretação dos resultados. Os valores de referência foram expressos em termos da média ± desvio-padrão, intervalo de confiança 95% e valor de referência superior. A distribuição estatística dos resultados obtidos foi realizada para possibilitar sua comparação com grupos de trabalhadores, preferentemente à avaliação individual. RESULTADOS E CONCLUSÕES: O valor médio ± desvio-padrão para a carboxiemoglobina foi de 1,0 % ± 0,75; o intervalo de confiança 95%, entre 0,9 e 1,1 % e o valor de referência superior, de 2,5%. Através do teste t de Student (p < 0,05 não foi detectada diferença nos valores de acordo com o sexo, idade ou uso de bebidas alcoólicas. Os valores de referência encontrados foram similares aos reportados em outros países.

  3. Aprender ciencias en y para la comunidad

    OpenAIRE

    Roth, Wolff-Michael

    2002-01-01

    Durante algún tiempo, he afirmado que la educación científica necesita ser desinstitucionalizada para superar la profunda crisis que atraviesa actualmente. En este artículo describo formas en las cuales se puede pensar y poner en práctica esta desinstitucionalización en la enseñanza de las ciencias y en la práctica del diseño del currículo de ciencias. Se propone la teoría de la actividad como marco para conceptualizar diferentes sistemas de actividad y sus contradicciones. Proporciono ejempl...

  4. Espacio urbano para el desarrollo sostenible

    Directory of Open Access Journals (Sweden)

    Guillermo Malca Obregoso

    2015-04-01

    Full Text Available Este artículo ilustra una Metodología de Análisis-Propuesta Urbana Sostenible para la enseñanza-aprendizaje del diseño urbano-arquitectónico conciente de su entorno socio-cultural-ambiental. Se presenta como alternativa a los métodos convencionales de intervención urbana. Pretende, a partir de la visión dialéctica, entender los problemas originados por el crecimiento de las ciudades de modo INTEGRAL, sin segmentarlos para su estudio parcializado y parcelado.

  5. T I C : Trayectos Interactivos para Compartir

    OpenAIRE

    Rodríguez Carracedo, María del Carmen

    2012-01-01

    Las tecnologías y sus acelerados desarrollos han tenido un considerable impacto social. La condición de nativos digitales –cada vez más presente- de nuestros alumnos, hace que espontáneamente convivan con esas herramientas y este mundo revolucionariamente cambiante. Sin embargo, no los convierte en innatos poseedores de habilidades para resolver conflictos cotidianos ni para el aprovechamiento apropiado de aquellas. Los sistemas educativos evolucionaron a un ritmo mucho más lento, pero ...

  6. Acessibilidade de museus europeus para deficientes visuais

    OpenAIRE

    Mesquita, Susana Maria Vasconcelos

    2011-01-01

    As pessoas portadoras de deficiência (física, auditiva, mental ou visual) têm as mesmas necessidades e desejos de participar e praticar turismo que o público dito normal. No entanto, esta tarefa, representa para este grupo um desafio acrescido, já que a nossa sociedade está pensada para a maioria das pessoas, que não são portadoras destas deficiências. Num mundo com imensos estímulos visuais existe necessidade de implementar estratégias que permitam às pessoas com deficiênci...

  7. Sistemas de labranza para suelos salinos

    OpenAIRE

    Hervé, Dominique; Mita, V.; Paz, B.

    2001-01-01

    Mejorar los sistemas de labranza para cultivar suelos salinos es un reto para el sur del Altiplano boliviano. Se comparo durante dos anos (1994-95 y 1995-96) el surcado en faja con discos, practicado por los campesinos de la provincia Villarroel, y el subsolado a 50 cm de profundidad solo o combinado con el surcado. Se buscaba asi romper la costra en suelos muy arcillosos y llevar las sales a mayor profundiad que los horizontes colonizados por las raices de los cultivos de quinua (raiz pivota...

  8. Neurocisticercosis: Afiches para Capacitación

    OpenAIRE

    MARCELO ROJAS C

    2007-01-01

    En el sistema universitario (me atrevo a decir, de todo el mundo), es poco el contenido educativo relativo a la Transferencia tecnológica para efectos de la capacitación de la sociedad sobre problemas de salud (claros ejemplos desafiantes para los veterinarios son la hidatidosis y cisticercosis) y otros; manejados en términos de cambios de conducta. Los términos de control y prevención están dados en un lenguaje académico, muy abstracto y obviamente intrascedente en la comunidad. Al punto que...

  9. Herramientas de software para OAI-PMH

    OpenAIRE

    Bueno-de-la-Fuente, Gema; Rodríguez-Mateos, David

    2007-01-01

    Tanto para poner en marcha proveedores de datos como proveedores de servicios o agregadores, es importante conocer cuáles son las alternativas de software entre las que elegir. Aunque en los capítulos anteriores se dan algunas indicaciones, se ha considerado oportuno dedicar el capítulo 8, “Herramientas de software para OAI-PMH”, a elaborar una descripción de las principales aplicaciones empleadas en la actualidad. Tras una primera introducción sobre la diferencia entre software libre, softwa...

  10. Indicadores de calidad para evaluar repositorios institucionales

    OpenAIRE

    Barrionuevo Almuzara, Leticia

    2010-01-01

    Se presentan una serie de indicadores útiles para garantizar la calidad de los repositorios institucionales en abierto. Evaluar o medir el valor de un producto, servicio, actividad u objeto es necesario para saber si éste está funcionando de acuerdo a unos criterios preestablecidos o por el contrario se deben corregir errores. Se puede hablar de una clara tendencia o moda de evaluación en los sistemas bibliotecarios, quizás por el carácter tan crítico de los clientes/usuarios, quienes desean ...

  11. Taller para padres en Centro CAES

    OpenAIRE

    Beltrán Franch, Enrique

    2015-01-01

    Treball final de Grau en Mestre o Mestra d'Educació Infantil. Codi: MI1040. Curs acadèmic 2014-2015 Este trabajo versa sobre las distintas fases que se han llevado a cabo para la realización en la realización de un taller para padres, organizado en seis sesiones, tratando diferentes temas (centrados en la diada de relación padres hijos y en proceso de socialización que en ella se lleva) en cada sesión. La finalidad de este trabajo era tanto concienciar a los padres en la gran r...

  12. IR & FX Linked Note para inversionistas colombianos

    OpenAIRE

    Restrepo Montoya, Julián Esteban

    2015-01-01

    Los Productos Estructurados son instrumentos financieros que son construidos para cumplir ciertas condiciones y objetivos de rentabilidad y riesgo que tenga su operador o inversionista -- Este documento presenta la construcción de un producto estructurado de tasas de interés y tasa de cambio (IR & FX por sus siglas en inglés) para inversionistas Colombianos, que maximiza la rentabilidad y garantiza la protección del capital -- La construcción de este instrumento se realiza a través de la inve...

  13. Share!: Sistema para compartir contenidos multimedia

    OpenAIRE

    Cimas Bravo, Eduardo

    2015-01-01

    Este trabajo consiste en un sistema informático destinado a la compartición de contenidos multimedia entre grupos restringidos de usuarios. Los contenidos pueden ser imágenes, vídeos y audios. Este sistema se compone de dos aplicaciones, una aplicación web y una aplicación móvil para dispositivos Android. La esencia del sistema es proporcionar un mecanismo para compartir rápidamente contenidos multimedia entre usuarios. Los contenidos pueden estar ya en sus dispositivos móviles o ser capturad...

  14. Propuestas para combatir la contabilidad creativa

    Directory of Open Access Journals (Sweden)

    Oriol Amat Salas

    2004-01-01

    Full Text Available Los escándalos contables han reavivado el interés en combatir la contabilidad creativa. Este artículo analiza la naturaleza de esta problemática y los aspectos de la normativa que la afectan. Incluye el ejemplo de una empresa real para visualizar los efectos que los maquillajes contables, amparados en la normativa contable española y en las Normas Internacionales de Contabilidad, pueden provocar en el resultado. Al final, se proponen medidas para mejorar la credibilidad de la información financiera.

  15. Propuestas para combatir la contabilidad creativa

    OpenAIRE

    Oriol Amat Salas; Ester Oliveras

    2004-01-01

    Los escándalos contables han reavivado el interés en combatir la contabilidad creativa. Este artículo analiza la naturaleza de esta problemática y los aspectos de la normativa que la afectan. Incluye el ejemplo de una empresa real para visualizar los efectos que los maquillajes contables, amparados en la normativa contable española y en las Normas Internacionales de Contabilidad, pueden provocar en el resultado. Al final, se proponen medidas para mejorar la credibil...

  16. Especializaci??n para la empleabilidad eficiente, anotaciones para el postgrado en trabajo social

    OpenAIRE

    Alonso Alonso, Rosario; Julve Negre, Milagros

    2006-01-01

    La especializaci??n ofrecida en los Postgrados de Trabajo Social deber??a de introducir nuevos principios de organizaci??n para situar a los profesionales en una posici??n competitiva para facilitar la movilidad laboral, caracter??stica espec??fica en el sector. Al mismo tiempo, orientarse por la eficacia y el ??xito en la intervenci??n profesional. Para ello es necesario introducir el concepto de "pr??cticas de calidad" e incluir contenidos que no s??lo formen en competencias Te??rico- t??cn...

  17. Proyecto de domótica para vivienda nueva y para vivienda existente

    OpenAIRE

    Ruzo Parragués, Martín

    2007-01-01

    Este proyecto consiste en el detalle de una instalación domótica de acuerdo a unas necesidades de usuario determinadas. El trabajo se divide en dos partes principales: la primera detalla una solución para esta instalación domótica en el caso que la vivienda escogida sea de nueva construcción. La segunda, en cambio, propone una solución para el caso que la misma vivienda esté ya construida. Para ambas soluciones se proponen tecnologías diferentes y se presentan sus características princi...

  18. Consumo de bebidas para una vida saludable: recomendaciones para la población mexicana

    OpenAIRE

    Juan A Rivera; Onofre Muñoz-Hernández; Martín Rosas-Peralta; Aguilar-Salinas, Carlos A; Willett, Walter C.; Popkin, Barry M.

    2008-01-01

    El secretario de Salud convocó al Comité de Expertos para la elaboración de las Recomendaciones sobre el consumo de bebidas para la población mexicana ; la finalidad fue desarrollar lineamientos basados en evidencia científica para los consumidores, los profesionales de la salud y el sector gubernamental. Las prevalencias de sobrepeso, obesidad y diabetes han aumentado con rapidez en México y las bebidas representan la quinta parte de la energía que consumen los mexicanos. La evidencia señal...

  19. Infraestructura de comunicaciones para algoritmos colaborativos

    OpenAIRE

    Sopena Ballesteros, Manuel

    2013-01-01

    Este proyecto intenta crear un sistema cliente/servidor de comunicaciones para distintas máquinas paralelas en C++ utilizando WCF como tecnología en el servidor. Aquest projecte intenta crear un sistema client/servidor de comunicacions per a diferents màquines paral·leles en C++ utilitzant WCF com tecnologia al servidor.

  20. Educación para la paz

    Directory of Open Access Journals (Sweden)

    Federico Mayor Zaragoza

    2003-01-01

    Full Text Available La educación para la paz ha experimentado un desarrollo importante en los últimos 20 años, y el 2000 ha sido declarado Año Internacional para la Cultura de Paz. Exige un esfuerzo de pariicipación y una capacidad crítica para facilitar el paso de una cultura de guerra a un cultura de paz. Se trata de ayudar a todos los ciudadanos a observar y reflexionar sobre el conjunto de la tierra y de quienes la habitan. De escuchar a los jóvenes y ser escuchado. La educación para la paz ha de ser considerada como contenido transversal de la educación. La situación actual, los conflictos interétnicos e interculturales , la exclusión y la marginalidad, las sociedades pobres y las ricas son cuestiones que reclaman una educación par la paz. De la cultura de imposición hemos de pasar a la cultura del diálogo y parti-cipación, solidaridad, compromiso, tolerancia y respeto. Es necesario construir otro mundo en el que podamos asegurar a nuestros descendientes el futuro. La paz es un comportamiento, es traducir a la práctica los principios de con-vivencia, de solidaridad, de fraternidad. Sólo así «vendrá el amanecer (y brilla-rá mucha luz en nuestro camino»