WorldWideScience

Sample records for beacons

  1. Auditory evacuation beacons

    NARCIS (Netherlands)

    Wijngaarden, S.J. van; Bronkhorst, A.W.; Boer, L.C.

    2005-01-01

    Auditory evacuation beacons can be used to guide people to safe exits, even when vision is totally obscured by smoke. Conventional beacons make use of modulated noise signals. Controlled evacuation experiments show that such signals require explicit instructions and are often misunderstood. A new

  2. Quantum Dot Molecular Beacons for DNA Detection

    Science.gov (United States)

    Cady, Nathaniel C.

    Molecular beacons have become an important fluorescent probe for sequence-specific DNA detection. To improve the sensitivity and robustness of molecular beacon assays, fluorescent semiconductor quantum dots (QDs) are now being used as the fluorescent moiety for molecular beacon synthesis. Multiple linkage strategies can be used for attaching molecular beacon DNA to QDs, and multiple quenchers, including gold particles, can be used for fluorescence quenching. Covalent attachment of QDs to DNA can be achieved through amide linkage, and affinity-based attachment can be achieved with streptavidin-biotin linkage. We have shown that these linkage strategies can be used to successfully create quantum dot molecular beacons that can be used in DNA detection assays with high specificity.

  3. The ANTARES optical beacon system

    Science.gov (United States)

    Ageron, M.; Aguilar, J. A.; Albert, A.; Ameli, F.; Anghinolfi, M.; Anton, G.; Anvar, S.; Ardellier-Desages, F.; Aslanides, E.; Aubert, J.-J.; Auer, R.; Barbarito, E.; Basa, S.; Battaglieri, M.; Becherini, Y.; Beltramelli, J.; Bertin, V.; Bigi, A.; Billault, M.; Blaes, R.; de Botton, N.; Bouwhuis, M. C.; Bradbury, S. M.; Bruijn, R.; Brunner, J.; Burgio, G. F.; Busto, J.; Cafagna, F.; Caillat, L.; Calzas, A.; Capone, A.; Caponetto, L.; Carmona, E.; Carr, J.; Cartwright, S. L.; Castel, D.; Castorina, E.; Cavasinni, V.; Cecchini, S.; Ceres, A.; Charvis, P.; Chauchot, P.; Chiarusi, T.; Circella, M.; Colnard, C.; Compère, C.; Coniglione, R.; Cottini, N.; Coyle, P.; Cuneo, S.; Cussatlegras, A.-S.; Damy, G.; van Dantzig, R.; de Bonis, G.; de Marzo, C.; de Vita, R.; Dekeyser, I.; Delagnes, E.; Denans, D.; Deschamps, A.; Destelle, J.-J.; Dinkespieler, B.; Distefano, C.; Donzaud, C.; Drogou, J.-F.; Druillole, F.; Durand, D.; Ernenwein, J.-P.; Escoffier, S.; Falchini, E.; Favard, S.; Fehr, F.; Feinstein, F.; Ferry, S.; Fiorello, C.; Flaminio, V.; Fratini, K.; Fuda, J.-L.; Galeotti, S.; Gallone, J.-M.; Giacomelli, G.; Girard, N.; Gojak, C.; Goret, Ph.; Graf, K.; Hallewell, G.; Harakeh, M. N.; Hartmann, B.; Heijboer, A.; Heine, E.; Hello, Y.; Hernández-Rey, J. J.; Hößl, J.; Hoffman, C.; Hogenbirk, J.; Hubbard, J. R.; Jaquet, M.; Jaspers, M.; de Jong, M.; Jouvenot, F.; Kalantar-Nayestanaki, N.; Kappes, A.; Karg, T.; Katz, U.; Keller, P.; Kok, E.; Kok, H.; Kooijman, P.; Kopper, C.; Korolkova, E. V.; Kouchner, A.; Kretschmer, W.; Kruijer, A.; Kuch, S.; Kudryavstev, V. A.; Lagier, P.; Lahmann, R.; Lamanna, G.; Lamare, P.; Lambard, G.; Languillat, J.-C.; Laschinsky, H.; Lavalle, J.; Le Guen, Y.; Le Provost, H.; Le van Suu, A.; Lefèvre, D.; Legou, T.; Lelaizant, G.; Lim, G.; Lo Presti, D.; Loehner, H.; Loucatos, S.; Louis, F.; Lucarelli, F.; Lyashuk, V.; Marcelin, M.; Margiotta, A.; Masullo, R.; Mazéas, F.; Mazure, A.; McMillan, J. E.; Megna, R.; Melissas, M.; Migneco, E.; Milovanovic, A.; Mongelli, M.; Montaruli, T.; Morganti, M.; Moscoso, L.; Musumeci, M.; Naumann-Godo, M.; Naumann, C.; Niess, V.; Noble, T.; Olivetto, C.; Ostasch, R.; Palanque-Delabrouille, N.; Payre, P.; Peek, H.; Perez, A.; Petta, C.; Piattelli, P.; Pillet, R.; Pineau, J.-P.; Poinsignon, J.; Popa, V.; Pradier, T.; Racca, C.; Randazzo, N.; van Randwijk, J.; Real, D.; van Rens, B.; Réthoré, F.; Rewiersma, P.; Riccobene, G.; Rigaud, V.; Ripani, M.; Roca, V.; Roda, C.; Rolin, J. F.; Rose, H. J.; Rostovtsev, A.; Roux, J.; Ruppi, M.; Russo, G. V.; Rusydi, G.; Salesa, F.; Salomon, K.; Sapienza, P.; Schmitt, F.; Schuller, J.-P.; Shanidze, R.; Sokalski, I.; Spona, T.; Spurio, M.; van der Steenhoven, G.; Stolarczyk, T.; Streeb, K.; Sulak, L.; Taiuti, M.; Tamburini, C.; Tao, C.; Terreni, G.; Thompson, L. F.; Urbano, F.; Valdy, P.; Valente, V.; Vallage, B.; Vaudaine, G.; Venekamp, G.; Verlaat, B.; Vernin, P.; de Vries-Uiterweerd, G.; van Wijk, R.; Wijnker, G.; de Witt Huberts, P.; Wobbe, G.; de Wolf, E.; Yao, A.-F.; Zaborov, D.; Zaccone, H.; Zornoza, J. D.; Zúñiga, J.

    2007-08-01

    ANTARES is a neutrino telescope being deployed in the Mediterranean Sea. It consists of a three-dimensional array of photomultiplier tubes that can detect the Cherenkov light induced by charged particles produced in the interactions of neutrinos with the surrounding medium. High angular resolution can be achieved, in particular, when a muon is produced, provided that the Cherenkov photons are detected with sufficient timing precision. Considerations of the intrinsic time uncertainties stemming from the transit time spread in the photomultiplier tubes and the mechanism of transmission of light in sea water lead to the conclusion that a relative time accuracy of the order of 0.5 ns is desirable. Accordingly, different time calibration systems have been developed for the ANTARES telescope. In this article, a system based on Optical Beacons, a set of external and well-controlled pulsed light sources located throughout the detector, is described. This calibration system takes into account the optical properties of sea water, which is used as the detection volume of the ANTARES telescope. The design, tests, construction and first results of the two types of beacons, LED and laser-based, are presented.

  4. Contention Window Analysis for Beaconing in VANETs

    OpenAIRE

    Reinders, René; van Eenennaam, Martijn; Karagiannis, Georgios; Heijenk, Geert

    2011-01-01

    The recently standardised IEEE 802.11p Wireless Access in Vehicular Environment (WAVE) protocol is envisioned to be used for Vehicle-to-Vehicle and Vehicle-to-Infrastructure communications. We provide an analysis of 802.11p’s performance when used for the exchange of small status messages known as beacons. These beacons enable vehicles to establish a Cooperative Awareness from which many applications can draw their inputs. Applications have been proposed to increase safety, efficiency and com...

  5. Multiple beacons for supporting lunar landing navigation

    Science.gov (United States)

    Theil, Stephan; Bora, Leonardo

    2018-02-01

    The exploration and potential future exploitation of solar system bodies requires technologies for precise and safe landings. Current navigation systems for landing probes are relying on a combination of inertial and optical sensor measurements to determine the current flight state with respect to the target body and the desired landing site. With a future transition from single exploration missions to more frequent first exploration and then exploitation missions, the implementation and operation of these missions changes, since it can be expected that a ground infrastructure on the target body is available in the vicinity of the landing site. In a previous paper, the impact of a single ground-based beacon on the navigation performance was investigated depending on the type of radiometric measurements and on the location of the beacon with respect to the landing site. This paper extends this investigation on options for ground-based multiple beacons supporting the on-board navigation system. It analyzes the impact on the achievable navigation accuracy. For that purpose, the paper introduces briefly the existing navigation architecture based on optical navigation and its extension with radiometric measurements. The same scenario of lunar landing as in the previous paper is simulated. The results are analyzed and discussed. They show a single beacon at a large distance along the landing trajectory and multiple beacons close to the landing site can improve the navigation performance. The results show how large the landing area can be increased where a sufficient navigation performance is achieved using the beacons.

  6. Beach Advisory and Closing Online Notification (BEACON) system

    Data.gov (United States)

    U.S. Environmental Protection Agency — Beach Advisory and Closing Online Notification system (BEACON) is a colletion of state and local data reported to EPA about beach closings and advisories. BEACON is...

  7. Determining Locations by Use of Networks of Passive Beacons

    Science.gov (United States)

    Okino, Clayton; Gray, Andrew; Jennings, Esther

    2009-01-01

    Networks of passive radio beacons spanning moderate-sized terrain areas have been proposed to aid navigation of small robotic aircraft that would be used to explore Saturn s moon Titan. Such networks could also be used on Earth to aid navigation of robotic aircraft, land vehicles, or vessels engaged in exploration or reconnaissance in situations or locations (e.g., underwater locations) in which Global Positioning System (GPS) signals are unreliable or unavailable. Prior to use, it would be necessary to pre-position the beacons at known locations that would be determined by use of one or more precise independent global navigation system(s). Thereafter, while navigating over the area spanned by a given network of passive beacons, an exploratory robot would use the beacons to determine its position precisely relative to the known beacon positions (see figure). If it were necessary for the robot to explore multiple, separated terrain areas spanned by different networks of beacons, the robot could use a long-haul, relatively coarse global navigation system for the lower-precision position determination needed during transit between such areas. The proposed method of precise determination of position of an exploratory robot relative to the positions of passive radio beacons is based partly on the principles of radar and partly on the principles of radio-frequency identification (RFID) tags. The robot would transmit radar-like signals that would be modified and reflected by the passive beacons. The distance to each beacon would be determined from the roundtrip propagation time and/or round-trip phase shift of the signal returning from that beacon. Signals returned from different beacons could be distinguished by means of their RFID characteristics. Alternatively or in addition, the antenna of each beacon could be designed to radiate in a unique pattern that could be identified by the navigation system. Also, alternatively or in addition, sets of identical beacons could

  8. 78 FR 75392 - American Beacon Funds, et al.; Notice of Application

    Science.gov (United States)

    2013-12-11

    ... Asset Management LLC; (b) American Beacon Balanced Fund--the Manager, Barrow, Hanley, Mewhinney... Beacon Funds, et al.; Notice of Application December 5, 2013. AGENCY: Securities and Exchange Commission...) (order). Applicants: American Beacon Funds and American Beacon Select Funds (collectively, the ``Trusts...

  9. Do rufous hummingbirds (Selasphorus rufus) use visual beacons?

    Science.gov (United States)

    Hurly, T Andrew; Franz, Simone; Healy, Susan D

    2010-03-01

    Animals are often assumed to use highly conspicuous features of a goal to head directly to that goal ('beaconing'). In the field it is generally assumed that flowers serve as beacons to guide pollinators. Artificial hummingbird feeders are coloured red to serve a similar function. However, anecdotal reports suggest that hummingbirds return to feeder locations in the absence of the feeder (and thus the beacon). Here we test these reports for the first time in the field, using the natural territories of hummingbirds and manipulating flowers on a scale that is ecologically relevant to the birds. We compared the predictions from two distinct hypotheses as to how hummingbirds might use the visual features of rewards: the distant beacon hypothesis and the local cue hypothesis. In two field experiments, we found no evidence that rufous hummingbirds used a distant visual beacon to guide them to a rewarded location. In no case did birds abandon their approach to the goal location from a distance; rather they demonstrated remarkable accuracy of navigation by approaching to within about 70 cm of a rewarded flower's original location. Proximity varied depending on the size of the training flower: birds flew closer to a previously rewarded location if it had been previously signalled with a small beacon. Additionally, when provided with a beacon at a new location, birds did not fly directly to the new beacon. Taken together, we believe these data demonstrate that these hummingbirds depend little on visual characteristics to beacon to rewarded locations, but rather that they encode surrounding landmarks in order to reach the goal and then use the visual features of the goal as confirmation that they have arrived at the correct location.

  10. Pedestrian hybrid beacon guide : recommendations and case study.

    Science.gov (United States)

    2014-01-01

    A pedestrian hybrid beacon (PHB) is a traffic control : device similar to a European pedestrian signal : (PELICAN) that was imported to the US and adapted by : engineers in Arizona to increase motorists awareness of : pedestrian crossings at uncon...

  11. A heuristic evaluation of the Facebook's advertising tool beacon

    OpenAIRE

    Jamal, A; Cole, M

    2009-01-01

    Interface usability is critical to the successful adoption of information systems. The aim of this study is to evaluate interface of Facebook's advertising tool Beacon by using privacy heuristics [4]. Beacon represents an interesting case study because of the negative media and user backlash it received. The findings of heuristic evaluation suggest violation of privacy heuristics [4]. Here, analysis identified concerns about user choice and consent, integrity and security of data, and awarene...

  12. Multiple systems for spatial learning: dead reckoning and beacon homing in rats.

    Science.gov (United States)

    Shettleworth, Sara J; Sutton, Jennifer E

    2005-04-01

    Rats homed with food in a large lighted arena. Without visual cues, they used dead reckoning. When a beacon indicated the home, rats could also use the beacon. Homing did not differ in 2 groups of rats, 1 provided with the beacon and 1 without it; tests without the beacon gave no evidence that beacon learning overshadowed dead reckoning (Experiment 1). When the beacon was at the home for 1 group and in random locations for another, there was again no evidence of cue competition (Experiment 2). Dead reckoning experience did not block acquisition of beacon homing (Experiment 3). Beacon learning and dead reckoning do not compete for predictive value but acquire information in parallel and are used hierarchically.

  13. New frontiers of molecular beacons: signal amplification and nanomachines

    DEFF Research Database (Denmark)

    Della Vedova, Paolo

    2013-01-01

    by two complementary strands (stem), conjugated respectively with a fluorophore and a quencher. Upon hybridization with complementary target, the stem bonds break, parting the fluorophore from the quencher and resulting in a detectable signal. This thesis is divided in two major sections. The first...... section gives initially an overview of the molecular beacon technology usability and working principle. Later on the mechanism of molecular beacon’s internalization by SLO treatment was theoretically and experimentally investigated to determine optimal transfection condition and actual cell loading....... Finally the problem of signal generation by molecular beacons was tackled by developing two new methods. It was demonstrated that by carefully design of interacting beacons with a new geometrical configuration, it is possible to induce among them both a linear and an avalanche reaction which improve...

  14. Beacon communities' public health initiatives: a case study analysis.

    Science.gov (United States)

    Massoudi, Barbara L; Marcial, Laura H; Haque, Saira; Bailey, Robert; Chester, Kelley; Cunningham, Shellery; Riley, Amanda; Soper, Paula

    2014-01-01

    The Beacon Communities for Public Health (BCPH) project was launched in 2011 to gain a better understanding of the range of activities currently being conducted in population- and public health by the Beacon Communities. The project highlighted the successes and challenges of these efforts with the aim of sharing this information broadly among the public health community. The Beacon Community Program, designed to showcase technology-enabled, community-based initiatives to improve outcomes, focused on: building and strengthening health information technology (IT) infrastructure and exchange capabilities; translating investments in health IT to measureable improvements in cost, quality, and population health; and, developing innovative approaches to performance measurement, technology, and care delivery. Four multimethod case studies were conducted based on a modified sociotechnical framework to learn more about public health initiative implementation and use in the Beacon Communities. Our methodological approach included using document review and semistructured key informant interviews. NACCHO Model Practice Program criteria were used to select the public health initiatives included in the case studies. Despite differences among the case studies, common barriers and facilitators were found to be present in all areas of the sociotechnical framework application including structure, people, technology, tasks, overarching considerations, and sustainability. Overall, there were many more facilitators (range = 7-14) present for each Beacon compared to barriers (range = 4-6). Four influential promising practices were identified through the work: forging strong and sustainable partnerships; ensuring a good task-technology fit and a flexible and iterative design; fostering technology acceptance; and, providing education and demonstrating value. A common weakness was the lack of a framework or model for the Beacon Communities evaluation work. Sharing a framework or approach

  15. BEACON: automated tool for Bacterial GEnome Annotation ComparisON.

    Science.gov (United States)

    Kalkatawi, Manal; Alam, Intikhab; Bajic, Vladimir B

    2015-08-18

    Genome annotation is one way of summarizing the existing knowledge about genomic characteristics of an organism. There has been an increased interest during the last several decades in computer-based structural and functional genome annotation. Many methods for this purpose have been developed for eukaryotes and prokaryotes. Our study focuses on comparison of functional annotations of prokaryotic genomes. To the best of our knowledge there is no fully automated system for detailed comparison of functional genome annotations generated by different annotation methods (AMs). The presence of many AMs and development of new ones introduce needs to: a/ compare different annotations for a single genome, and b/ generate annotation by combining individual ones. To address these issues we developed an Automated Tool for Bacterial GEnome Annotation ComparisON (BEACON) that benefits both AM developers and annotation analysers. BEACON provides detailed comparison of gene function annotations of prokaryotic genomes obtained by different AMs and generates extended annotations through combination of individual ones. For the illustration of BEACON's utility, we provide a comparison analysis of multiple different annotations generated for four genomes and show on these examples that the extended annotation can increase the number of genes annotated by putative functions up to 27%, while the number of genes without any function assignment is reduced. We developed BEACON, a fast tool for an automated and a systematic comparison of different annotations of single genomes. The extended annotation assigns putative functions to many genes with unknown functions. BEACON is available under GNU General Public License version 3.0 and is accessible at: http://www.cbrc.kaust.edu.sa/BEACON/ .

  16. On the Efficiency of Secure Beaconing in VANETs

    NARCIS (Netherlands)

    Schoch, Elmar; Kargl, Frank

    Direct inter-vehicle communication enables numerous safety applications like intersection collision warning. Beacons - periodic one-hop link-layer broadcast messages containing, e.g., location, heading, and speed - are the basis for many such applications. For security, current work often requires

  17. Robot-Beacon Distributed Range-Only SLAM for Resource-Constrained Operation.

    Science.gov (United States)

    Torres-González, Arturo; Martínez-de Dios, Jose Ramiro; Ollero, Anibal

    2017-04-20

    This work deals with robot-sensor network cooperation where sensor nodes (beacons) are used as landmarks for Range-Only (RO) Simultaneous Localization and Mapping (SLAM). Most existing RO-SLAM techniques consider beacons as passive devices disregarding the sensing, computational and communication capabilities with which they are actually endowed. SLAM is a resource-demanding task. Besides the technological constraints of the robot and beacons, many applications impose further resource consumption limitations. This paper presents a scalable distributed RO-SLAM scheme for resource-constrained operation. It is capable of exploiting robot-beacon cooperation in order to improve SLAM accuracy while meeting a given resource consumption bound expressed as the maximum number of measurements that are integrated in SLAM per iteration. The proposed scheme combines a Sparse Extended Information Filter (SEIF) SLAM method, in which each beacon gathers and integrates robot-beacon and inter-beacon measurements, and a distributed information-driven measurement allocation tool that dynamically selects the measurements that are integrated in SLAM, balancing uncertainty improvement and resource consumption. The scheme adopts a robot-beacon distributed approach in which each beacon participates in the selection, gathering and integration in SLAM of robot-beacon and inter-beacon measurements, resulting in significant estimation accuracies, resource-consumption efficiency and scalability. It has been integrated in an octorotor Unmanned Aerial System (UAS) and evaluated in 3D SLAM outdoor experiments. The experimental results obtained show its performance and robustness and evidence its advantages over existing methods.

  18. The BEACON on-line core monitoring system. Functional upgrades and applications

    International Nuclear Information System (INIS)

    Boyd, W.A.; Miller, R.W.

    1997-01-01

    The BEACON TM core monitor system has been in commercial operation since 1989 and was licensed by the USNRC for on-line core power distribution and thermal power limit monitoring in 1994. Since that time BEACON has been installed at 17 plants. Each of these customers has a different perspective on the use of data from BEACON and a different approach on the application of BEACON to support their plant operations. To support these varied needs and approaches the BEACON system has been divided into three operational levels to better match the system functions to the customer needs and approaches to system integration. Based on customer feedback, the BEACON system was upgraded in some areas and streamlined in other areas to better support the needs of each customer. The three operational levels of the BEACON system, the major product upgrades and system evolution that has taken place to support the needs and applications of our customers are discussed. (authors)

  19. Smartphone-Based Indoor Localization with Bluetooth Low Energy Beacons.

    Science.gov (United States)

    Zhuang, Yuan; Yang, Jun; Li, You; Qi, Longning; El-Sheimy, Naser

    2016-04-26

    Indoor wireless localization using Bluetooth Low Energy (BLE) beacons has attracted considerable attention after the release of the BLE protocol. In this paper, we propose an algorithm that uses the combination of channel-separate polynomial regression model (PRM), channel-separate fingerprinting (FP), outlier detection and extended Kalman filtering (EKF) for smartphone-based indoor localization with BLE beacons. The proposed algorithm uses FP and PRM to estimate the target's location and the distances between the target and BLE beacons respectively. We compare the performance of distance estimation that uses separate PRM for three advertisement channels (i.e., the separate strategy) with that use an aggregate PRM generated through the combination of information from all channels (i.e., the aggregate strategy). The performance of FP-based location estimation results of the separate strategy and the aggregate strategy are also compared. It was found that the separate strategy can provide higher accuracy; thus, it is preferred to adopt PRM and FP for each BLE advertisement channel separately. Furthermore, to enhance the robustness of the algorithm, a two-level outlier detection mechanism is designed. Distance and location estimates obtained from PRM and FP are passed to the first outlier detection to generate improved distance estimates for the EKF. After the EKF process, the second outlier detection algorithm based on statistical testing is further performed to remove the outliers. The proposed algorithm was evaluated by various field experiments. Results show that the proposed algorithm achieved the accuracy of <2.56 m at 90% of the time with dense deployment of BLE beacons (1 beacon per 9 m), which performs 35.82% better than <3.99 m from the Propagation Model (PM) + EKF algorithm and 15.77% more accurate than <3.04 m from the FP + EKF algorithm. With sparse deployment (1 beacon per 18 m), the proposed algorithm achieves the accuracies of <3.88 m at 90% of the

  20. Smartphone-Based Indoor Localization with Bluetooth Low Energy Beacons

    Directory of Open Access Journals (Sweden)

    Yuan Zhuang

    2016-04-01

    Full Text Available Indoor wireless localization using Bluetooth Low Energy (BLE beacons has attracted considerable attention after the release of the BLE protocol. In this paper, we propose an algorithm that uses the combination of channel-separate polynomial regression model (PRM, channel-separate fingerprinting (FP, outlier detection and extended Kalman filtering (EKF for smartphone-based indoor localization with BLE beacons. The proposed algorithm uses FP and PRM to estimate the target’s location and the distances between the target and BLE beacons respectively. We compare the performance of distance estimation that uses separate PRM for three advertisement channels (i.e., the separate strategy with that use an aggregate PRM generated through the combination of information from all channels (i.e., the aggregate strategy. The performance of FP-based location estimation results of the separate strategy and the aggregate strategy are also compared. It was found that the separate strategy can provide higher accuracy; thus, it is preferred to adopt PRM and FP for each BLE advertisement channel separately. Furthermore, to enhance the robustness of the algorithm, a two-level outlier detection mechanism is designed. Distance and location estimates obtained from PRM and FP are passed to the first outlier detection to generate improved distance estimates for the EKF. After the EKF process, the second outlier detection algorithm based on statistical testing is further performed to remove the outliers. The proposed algorithm was evaluated by various field experiments. Results show that the proposed algorithm achieved the accuracy of <2.56 m at 90% of the time with dense deployment of BLE beacons (1 beacon per 9 m, which performs 35.82% better than <3.99 m from the Propagation Model (PM + EKF algorithm and 15.77% more accurate than <3.04 m from the FP + EKF algorithm. With sparse deployment (1 beacon per 18 m, the proposed algorithm achieves the accuracies of <3.88 m at

  1. BEACON: automated tool for Bacterial GEnome Annotation ComparisON

    KAUST Repository

    Kalkatawi, Manal M.

    2015-08-18

    Background Genome annotation is one way of summarizing the existing knowledge about genomic characteristics of an organism. There has been an increased interest during the last several decades in computer-based structural and functional genome annotation. Many methods for this purpose have been developed for eukaryotes and prokaryotes. Our study focuses on comparison of functional annotations of prokaryotic genomes. To the best of our knowledge there is no fully automated system for detailed comparison of functional genome annotations generated by different annotation methods (AMs). Results The presence of many AMs and development of new ones introduce needs to: a/ compare different annotations for a single genome, and b/ generate annotation by combining individual ones. To address these issues we developed an Automated Tool for Bacterial GEnome Annotation ComparisON (BEACON) that benefits both AM developers and annotation analysers. BEACON provides detailed comparison of gene function annotations of prokaryotic genomes obtained by different AMs and generates extended annotations through combination of individual ones. For the illustration of BEACON’s utility, we provide a comparison analysis of multiple different annotations generated for four genomes and show on these examples that the extended annotation can increase the number of genes annotated by putative functions up to 27 %, while the number of genes without any function assignment is reduced. Conclusions We developed BEACON, a fast tool for an automated and a systematic comparison of different annotations of single genomes. The extended annotation assigns putative functions to many genes with unknown functions. BEACON is available under GNU General Public License version 3.0 and is accessible at: http://www.cbrc.kaust.edu.sa/BEACON/

  2. Caged molecular beacons: controlling nucleic acid hybridization with light.

    Science.gov (United States)

    Wang, Chunming; Zhu, Zhi; Song, Yanling; Lin, Hui; Yang, Chaoyong James; Tan, Weihong

    2011-05-28

    We have constructed a novel class of light-activatable caged molecular beacons (cMBs) that are caged by locking two stems with a photo-labile biomolecular interaction or covalent bond. With the cMBs, the nucleic acid hybridization process can be easily controlled with light, which offers the possibility for a high spatiotemporal resolution study of intracellular mRNAs. © The Royal Society of Chemistry 2011

  3. Performance of MAC protocols in beaconing Mobile Adhoc Multibroadcast Networks

    OpenAIRE

    Rico García, Cristina; Lehner, Andreas; Robertson, Patrick; Strang, Thomas

    2010-01-01

    The investigation of infrastructureless safety applications in dierent transportation systems is a hot research topic. The nodes in thevnetwork are designed to advertise to the rest of the nodes informationabout the current traffic situation by means of short beacon messages containing speed, direction, positions and other relevant safety information. The scheduling should be organized by the MAC layer so that the transmitted messages arrive successfully as soon as possible at the recei...

  4. Adapting Mobile Beacon-Assisted Localization in Wireless Sensor Networks

    Directory of Open Access Journals (Sweden)

    Wei Dong

    2009-04-01

    Full Text Available The ability to automatically locate sensor nodes is essential in many Wireless Sensor Network (WSN applications. To reduce the number of beacons, many mobile-assisted approaches have been proposed. Current mobile-assisted approaches for localization require special hardware or belong to centralized localization algorithms involving some deterministic approaches due to the fact that they explicitly consider the impreciseness of location estimates. In this paper, we first propose a range-free, distributed and probabilistic Mobile Beacon-assisted Localization (MBL approach for static WSNs. Then, we propose another approach based on MBL, called Adapting MBL (A-MBL, to increase the efficiency and accuracy of MBL by adapting the size of sample sets and the parameter of the dynamic model during the estimation process. Evaluation results show that the accuracy of MBL and A-MBL outperform both Mobile and Static sensor network Localization (MSL and Arrival and Departure Overlap (ADO when both of them use only a single mobile beacon for localization in static WSNs.

  5. Adapting mobile beacon-assisted localization in wireless sensor networks.

    Science.gov (United States)

    Teng, Guodong; Zheng, Kougen; Dong, Wei

    2009-01-01

    The ability to automatically locate sensor nodes is essential in many Wireless Sensor Network (WSN) applications. To reduce the number of beacons, many mobile-assisted approaches have been proposed. Current mobile-assisted approaches for localization require special hardware or belong to centralized localization algorithms involving some deterministic approaches due to the fact that they explicitly consider the impreciseness of location estimates. In this paper, we first propose a range-free, distributed and probabilistic Mobile Beacon-assisted Localization (MBL) approach for static WSNs. Then, we propose another approach based on MBL, called Adapting MBL (A-MBL), to increase the efficiency and accuracy of MBL by adapting the size of sample sets and the parameter of the dynamic model during the estimation process. Evaluation results show that the accuracy of MBL and A-MBL outperform both Mobile and Static sensor network Localization (MSL) and Arrival and Departure Overlap (ADO) when both of them use only a single mobile beacon for localization in static WSNs.

  6. Detection of Toxoplasma gondii with a DNA molecular beacon probe

    Science.gov (United States)

    Zhou, Cun; Xu, Shichao; Yang, Juan; Zhang, Jimei; Dai, Zhao; Zheng, Guo; Sun, Bo; Sun, Shuqing; Feng, Teilin; Zi, Yan; Liang, Chu; Luo, Hao

    2009-07-01

    Toxoplasma gondii is a kind of microscopic parasite that may infect humans, and there are increasing concerns on the early detection of latent Toxoplasma gondii infection in recent years. This research highlights a new type of molecular beacon (MB) fluorescent probe for Toxoplasma DNA testing. We combined high-efficiency fluorescent inorganic core-shell quantum dots-CdTe/ZnS (as fluorescent energy donor) and BHQ-2 (energy acceptor) to the single-strand DNA of Toxoplasma gondii, and a molecular beacon sensing system based on fluorescence resonance energy transfer (FRET) was achieved. Core-shell quantum dots CdTe/ZnS was firstly prepared in aqueous solution, and the influencing factor of its fluorescent properties, including CdTe/Na2S/Zn(CH3COO)2 (v/v), dependence of reaction time, temperature, and pH, is investigated systematically. The synthesized quantum dots and molecular beacon were characterized by transmission electron microscopy (TEM), ultraviolet-visible spectrophotometer (UV-vis), fluorescent spectrophotometer (FS), respectively. The TEM results showed that CdTe/ZnS core-shell quantum dots is ~11nm in size, and the quantum dots is water-soluble well. The sensing ability of target DNA of assembled MB was investigated, and results showed that the target Toxoplasma gonddi DNA can be successfully detected by measuring the change of fluorescence intensity. The results showed that the current sensing probe will be a useful and convenient tool in Toxoplasma gondii early detection.

  7. An Analysis of the Optimal Placement of Beacon in Bluetooth-INS Indoor Localization

    OpenAIRE

    Zhao, Xinyu; Ruan, Ling; Zhang, Ling; Long, Yi; Cheng, Fei

    2018-01-01

    The placement of Bluetooth beacon has immediate impact on the accuracy and stability of indoor positioning. Affected by the shelter of building and human, Bluetooth shows uncertain spatial transmission characteristics. Therefore, the scientific deployment of the beacon nodes is closely related to the indoor space environment. In the study of positioning technology using Bluetooth, some scholars have discussed the deployment of Bluetooth beacon in different scenarios. In the principle of avoid...

  8. Weak beacon detection for air-to-ground optical wireless link establishment.

    Science.gov (United States)

    Han, Yaoqiang; Dang, Anhong; Tang, Junxiong; Guo, Hong

    2010-02-01

    In an air-to-ground free-space optical communication system, strong background interference seriously affects the beacon detection, which makes it difficult to establish the optical link. In this paper, we propose a correlation beacon detection scheme under strong background interference conditions. As opposed to traditional beacon detection schemes, the beacon is modulated by an m-sequence at the transmitting terminal with a digital differential matched filter (DDMF) array introduced at the receiving end to detect the modulated beacon. This scheme is capable of suppressing both strong interference and noise by correlation reception of the received image sequence. In addition, the DDMF array enables each pixel of the image sensor to have its own DDMF of the same structure to process its received image sequence in parallel, thus it makes fast beacon detection possible. Theoretical analysis and an outdoor experiment have been demonstrated and show that the proposed scheme can realize fast and effective beacon detection under strong background interference conditions. Consequently, the required beacon transmission power can also be reduced dramatically.

  9. Beacon- and Schema-Based Method for Recognizing Algorithms from Students' Source Code

    Science.gov (United States)

    Taherkhani, Ahmad; Malmi, Lauri

    2013-01-01

    In this paper, we present a method for recognizing algorithms from students programming submissions coded in Java. The method is based on the concept of "programming schemas" and "beacons". Schemas are high-level programming knowledge with detailed knowledge abstracted out, and beacons are statements that imply specific…

  10. Crowdsourcing for Context: Regarding Privacy in Beacon Encounters via Contextual Integrity

    Directory of Open Access Journals (Sweden)

    Bello-Ogunu Emmanuel

    2016-07-01

    Full Text Available Research shows that context is important to the privacy perceptions associated with technology. With Bluetooth Low Energy beacons, one of the latest technologies for providing proximity and indoor tracking, the current identifiers that characterize a beacon are not sufficient for ordinary users to make informed privacy decisions about the location information that could be shared. One solution would be to have standardized category and privacy labels, produced by beacon providers or an independent third-party. An alternative solution is to find an approach driven by users, for users. In this paper, we propose a novel crowdsourcing based approach to introduce elements of context in beacon encounters.We demonstrate the effectiveness of this approach through a user study, where participants use a crowd-based mobile app designed to collect beacon category and privacy information as a scavenger hunt game. Results show that our approach was effective in helping users label beacons according to the specific context of a given beacon encounter, as well as the privacy perceptions associated with it. This labeling was done with an accuracy of 92%, and with an acceptance rate of 82% of all recommended crowd labels. Lastly, we conclusively show how crowdsourcing for context can be used towards a user-centric framework for privacy management during beacon encounters.

  11. Determination of BEACON Coupling Coefficients using data from Xenon transient

    International Nuclear Information System (INIS)

    Bozic, M.; Kurincic, B.

    2007-01-01

    NEK uses BEACO TM code (BEACO TM - Westinghouse Best Estimate Analyzer for Core Operating Nuclear) for core monitoring, analysis and core behaviour prediction. Coupling Coefficients determine relationship between core response and excore instrumentation. Measured power distribution using incore moveable detectors during Xenon transient with sufficient power axial offset change is the most important data for further analysis. Classic methodology and BEACO TM Conservative methodology using established Coupling Coefficients are compared on NPP Krsko case. BEACON TM Conservative methodology with predefined Coupling Coefficients is used as a surveillance tool for verification of relationship between core and excore instrumentation during power operation. (author)

  12. The tick (Acari: Ixodidae) fauna of Herald's Beacon Islet, Australia.

    Science.gov (United States)

    Kwak, Mackenzie L; Mintram, Kate

    2017-01-01

    A rare opportunity to travel to Herald's Beacon Islet with permission from the Australian government to collect ticks allowed for a survey of the tick fauna of the island to be undertaken for the first time. The avian fauna of the island, which serve as hosts, was also recorded and includes one new species record for the island. The seabird soft tick Ornithodoros capensis Neumann and the seabird hard tick Amblyomma loculosum Neumann were found to be present on the island. Images of the ticks present on the island are presented along with morphological characters for their identification.

  13. Deep sea AUV navigation using multiple acoustic beacons

    Science.gov (United States)

    Ji, Da-xiong; Song, Wei; Zhao, Hong-yu; Liu, Jian

    2016-04-01

    Navigation is a critical requirement for the operation of Autonomous Underwater Vehicles (AUVs). To estimate the vehicle position, we present an algorithm using an extended Kalman filter (EKF) to integrate dead-reckoning position with acoustic ranges from multiple beacons pre-deployed in the operating environment. Owing to high latency, variable sound speed multipath transmissions and unreliability in acoustic measurements, outlier recognition techniques are proposed as well. The navigation algorithm has been tested by the recorded data of deep sea AUV during field operations in a variety of environments. Our results show the improved performance over prior techniques based on position computation.

  14. Design and implementation of Bluetooth beacon in mobile payment system

    Science.gov (United States)

    Han, Tiantian; Ding, Lei

    2017-08-01

    The current line of payment means, mainly in the following ways, cash payment, credit card payment, WeChat Alipay sweep payment. There are many inconvenience in Cash payment, large amounts of cash inconvenience to carry, count the money to spend time and effort, true and false banknotes difficult to distinguish, ticket settlement easy to go wrong. Credit card payment is relatively time-consuming, and WeChat Alipay sweep payment need to sweep. Therefore, the design of a convenient, fast payment to meet the line to pay the demand is particularly important. Based on the characteristics of BLE Bluetooth wireless communication technology, this paper designs a kind of payment method based on Bluetooth beacon. Through the Bluetooth beacon broadcast consumption, consumers only need to open the relevant APP in the Android client, and you can get Bluetooth via mobile phone Bluetooth the amount of consumption of the standard broadcast, in accordance with the corresponding payment platform to complete the payment process, which pay less time to improve the efficiency of payment.

  15. Pedestrian and motorists' actions at pedestrian hybrid beacon sites: findings from a pilot study.

    Science.gov (United States)

    Pulugurtha, Srinivas S; Self, Debbie R

    2015-01-01

    This paper focuses on an analysis of pedestrian and motorists' actions at sites with pedestrian hybrid beacons and assesses their effectiveness in improving the safety of pedestrians. Descriptive and statistical analyses (one-tail two-sample T-test and two-proportion Z-test) were conducted using field data collected during morning and evening peak hours at three study sites in the city of Charlotte, NC, before and after the installation of pedestrian hybrid beacons. Further, an analysis was conducted to assess the change in pedestrian and motorists' actions over time (before the installation; 1 month, 3 months, 6 months, and 12 months after the installation). Results showed an increase in average traffic speed at one of the pedestrian hybrid beacon sites while no specific trends were observed at the other two pedestrian hybrid beacon sites. A decrease in the number of motorists not yielding to pedestrians, pedestrians trapped in the middle of the street, and pedestrian-vehicle conflicts were observed at all the three pedestrian hybrid beacon sites. The installation of pedestrian hybrid beacons did not have a negative effect on pedestrian actions at two out of the three sites. Improvements seem to be relatively more consistent 3 months after the installation of the pedestrian hybrid beacon.

  16. Tight Bounds for Beacon-Based Coverage in Simple Rectilinear Polygons

    KAUST Repository

    Bae, Sang Won

    2016-03-21

    We establish tight bounds for beacon-based coverage problems. In particular, we show that $$\\\\lfloor \\\\frac{n}{6} \\ floor $$⌊n6⌋ beacons are always sufficient and sometimes necessary to cover a simple rectilinear polygon P with n vertices. When P is monotone and rectilinear, we prove that this bound becomes $$\\\\lfloor \\\\frac{n+4}{8} \\ floor $$⌊n+48⌋. We also present an optimal linear-time algorithm for computing the beacon kernel of P.

  17. Detecting and Preventing Beacon Replay Attacks in Receiver-Initiated MAC Protocols for Energy Efficient WSNs

    DEFF Research Database (Denmark)

    Di Mauro, Alessio; Fafoutis, Xenofon; Mödersheim, Sebastian Alexander

    2013-01-01

    nodes, pretending to have a fake identity within the network. To prevent this attack we propose RAP, a challenge-response authentication protocol that is able to detect and prevent the beacon replay attack. The effectiveness of the protocol is formally verified using OFMC and ProVerif. Furthermore, we......In receiver-initiated MAC protocols for Wireless Sensor Networks (WSNs), communication is initiated by the receiver of the data through beacons containing the receiver's identity. In this paper, we consider the case of a network intruder that captures and replays such beacons towards legitimate...

  18. Seamless Guidance System Combining GPS, BLE Beacon, and NFC Technologies

    Directory of Open Access Journals (Sweden)

    Rung-Shiang Cheng

    2016-01-01

    Full Text Available Users rely increasingly on Location-Based Services (LBS and automated navigation/guidance systems nowadays. However, while such services are easily implemented in outdoor environments using Global Positioning System (GPS technology, a requirement still exists for accurate localization and guidance schemes in indoor settings. Accordingly, the present study proposes a system based on GPS, Bluetooth Low Energy (BLE beacons, and Near Field Communication (NFC technology. Through establishing graphic information and the design of algorithm, this study develops a guidance system for indoors and outdoors on smart phones, wishing to give user perfect smart life through this system. The proposed system is implemented on a smart phone and evaluated on a student campus environment. The experimental results confirm the ability of the proposed app to switch automatically from an outdoor mode to an indoor mode and to guide the user to requested target destination via the shortest possible route.

  19. Multi-kW Uplink Fiber-Laser Beacon with Agile Signal Format Project

    Data.gov (United States)

    National Aeronautics and Space Administration — Uplink Laser Beacons for deep-space communication, can benefit greatly from migration to the 1010-1030nm wavelengths, via use of Silicon-APDs on the spacecraft...

  20. Multi-kW Uplink Fiber-Laser Beacon with Agile Signal Format Project

    Data.gov (United States)

    National Aeronautics and Space Administration —  Laser beacons with scalable powers are needed for ground to deep-space optical communication uplinks. They serve as absolute reference for tracking of...

  1. Evaluation of light-emitting diode beacon light fixtures : final report.

    Science.gov (United States)

    2009-12-01

    Rotating beacons containing filament light sources have long been used on highway maintenance trucks : to indicate the presence of the truck to other drivers. Because of advances in light-emitting diode (LED) : technologies, flashing lights containin...

  2. Optimal placement of range-only beacons for mobile robot localisation

    CSIR Research Space (South Africa)

    Burke, Michael G

    2011-11-01

    Full Text Available -only Beacons for Mobile Robot Localisation Michael Burke Mobile Intelligent Autonomous Systems Council for Scientific and Industrial Research Pretoria, South Africa Email: michaelburke@ieee.org Nico Bos Dept. Electrical, Electronic and Computer...

  3. NPP Grassland: Beacon Hill, U.K., 1972-1993, R1

    Data.gov (United States)

    National Aeronautics and Space Administration — This data set contains two ASCII text files, one providing productivity measurements for a chalk grassland on Beacon Hill, West Sussex, U.K. (50.92 N, -0.85 W) and...

  4. Multi-kW Uplink Fiber-Laser Beacon with Agile Signal Format, Phase II

    Data.gov (United States)

    National Aeronautics and Space Administration —  Laser beacons with scalable powers are needed for ground to deep-space optical communication uplinks. They serve as absolute reference for tracking of spacecraft...

  5. Multi-kW Uplink Fiber-Laser Beacon with Agile Signal Format, Phase I

    Data.gov (United States)

    National Aeronautics and Space Administration — Uplink Laser Beacons for deep-space communication, can benefit greatly from migration to the 1010-1030nm wavelengths, via use of Silicon-APDs on the spacecraft...

  6. High-throughput SNP genotyping: combining tag SNPs and molecular beacons

    CSIR Research Space (South Africa)

    Barreiro, LB

    2009-10-01

    Full Text Available In the last decade, molecular beacons have emerged to become a widely used tool in the multiplex typing of single nucleotide polymorphisms (SNPs). Improvements in detection technologies in instrumentation and chemistries to label these probes have...

  7. 76 FR 65216 - Beacon Medical Services, LLC, Aurora, CO; Notice of Negative Determination Regarding Application...

    Science.gov (United States)

    2011-10-20

    ... coders'' at Beacon Medical Services, LLC, Aurora, Colorado, states that ``our jobs were outsourced to... shift in production by such workers' firm or subdivision to a foreign country of articles like or...

  8. Bluetooth Low Energy Beacon Performance and Utility in a Car Location Memory Aid Setting

    OpenAIRE

    Sætre, Karsten Andre Skogstad

    2018-01-01

    This project explores the usefulness and performance of BLE Bluetooth beacons. This is done through an experiment conducted in cooperation with a fellow student. The results reveal weaknesses in performance, but also potential. Within the constraints of those weaknesses I construct a memory aid prototype Android application, CaRemember, as a proof of concept in order to demonstrate a potentially useful use case that make use of BLE Bluetooth beacons.

  9. Quick acquisition and recognition method for the beacon in deep space optical communications.

    Science.gov (United States)

    Wang, Qiang; Liu, Yuefei; Ma, Jing; Tan, Liying; Yu, Siyuan; Li, Changjiang

    2016-12-01

    In deep space optical communications, it is very difficult to acquire the beacon given the long communication distance. Acquisition efficiency is essential for establishing and holding the optical communication link. Here we proposed a quick acquisition and recognition method for the beacon in deep optical communications based on the characteristics of the deep optical link. To identify the beacon from the background light efficiently, we utilized the maximum similarity between the collecting image and the reference image for accurate recognition and acquisition of the beacon in the area of uncertainty. First, the collecting image and the reference image were processed by Fourier-Mellin. Second, image sampling and image matching were applied for the accurate positioning of the beacon. Finally, the field programmable gate array (FPGA)-based system was used to verify and realize this method. The experimental results showed that the acquisition time for the beacon was as fast as 8.1s. Future application of this method in the system design of deep optical communication will be beneficial.

  10. Accelerated Adoption of Advanced Health Information Technology in Beacon Community Health Centers.

    Science.gov (United States)

    Jones, Emily; Wittie, Michael

    2015-01-01

    To complement national and state-level HITECH Act programs, 17 Beacon communities were funded to fuel community-wide use of health information technology to improve quality. Health centers in Beacon communities received supplemental funding. This article explores the association between participation in the Beacon program and the adoption of electronic health records. Using the 2010-2012 Uniform Data System, trends in health information technology adoption among health centers located within and outside of Beacon communities were explored using differences in mean t tests and multivariate logistic regression. Electronic health record adoption was widespread and rapidly growing in all health centers, especially quality improvement functionalities: structured data capture, order and results management, and clinical decision support. Adoption lagged for functionalities supporting patient engagement, performance measurement, care coordination, and public health. The use of advanced functionalities such as care coordination grew faster in Beacon health centers, and Beacon health centers had 1.7 times higher odds of adopting health records with basic safety and quality functionalities in 2010-2012. Three factors likely underlie these findings: technical assistance, community-wide activation supporting health information exchange, and the layering of financial incentives. Additional technical assistance and community-wide activation is needed to support the use of functionalities that are currently lagging. © Copyright 2015 by the American Board of Family Medicine.

  11. Visual detection of multidrug resistance gene in living cell using the molecular beacon imaging

    Science.gov (United States)

    Zhou, Qiumei; Ma, Yi; Gu, Yueqing

    2014-09-01

    A major problem in cancer treatment is the development of resistance to chemotherapeutic agents in tumor cells. Detection of effective prognostic biomarkers and targets are of crucial importance to the management of individualized therapies. However, quantitative analysis of the drug resistance gene had been difficult because of technical limitations. In this study, we designed and used a special hairpin deoxyribonucleic acid (DNA), which served as a beacon for detecting human drug resistance indicater. Upon hybridizing with the target mRNA, the hairpin DNA modified gold nanoparticle beacons (hDAuNP beacons) release the fluorophores attached at 5'end of the oligonucleotide sequence. The fluorescence properties of the beacon before and after the hybridization with the complementary DNA were confirmed in vitro. The hDAuNP beacons could be taken up by living cells with low inherent cytotoxicity and higher stability. hDAuNP beacon imaged by confocal laser scanning microscopy to detect the resistance gene expression. The detected fluorescence in MCF7and MCF7/ADR cells correlates with the specific drug resistance gene expression, which is consistent with the result from Q-PCR. Thus, this approach overcame many of the challenges of previous techniques by creating highly sensitive and effective intracellular probes for monitoring gene expression.

  12. An Adaptive Scheme for Robot Localization and Mapping with Dynamically Configurable Inter-Beacon Range Measurements

    Directory of Open Access Journals (Sweden)

    Arturo Torres-González

    2014-04-01

    Full Text Available This work is motivated by robot-sensor network cooperation techniques where sensor nodes (beacons are used as landmarks for range-only (RO simultaneous localization and mapping (SLAM. This paper presents a RO-SLAM scheme that actuates over the measurement gathering process using mechanisms that dynamically modify the rate and variety of measurements that are integrated in the SLAM filter. It includes a measurement gathering module that can be configured to collect direct robot-beacon and inter-beacon measurements with different inter-beacon depth levels and at different rates. It also includes a supervision module that monitors the SLAM performance and dynamically selects the measurement gathering configuration balancing SLAM accuracy and resource consumption. The proposed scheme has been applied to an extended Kalman filter SLAM with auxiliary particle filters for beacon initialization (PF-EKF SLAM and validated with experiments performed in the CONET Integrated Testbed. It achieved lower map and robot errors (34% and 14%, respectively than traditional methods with a lower computational burden (16% and similar beacon energy consumption.

  13. Practical Fingerprinting Localization for Indoor Positioning System by Using Beacons

    Directory of Open Access Journals (Sweden)

    Santosh Subedi

    2017-01-01

    Full Text Available Recent developments in the fields of smartphones and wireless communication technologies such as beacons, Wi-Fi, and ultra-wideband have made it possible to realize indoor positioning system (IPS with a few meters of accuracy. In this paper, an improvement over traditional fingerprinting localization is proposed by combining it with weighted centroid localization (WCL. The proposed localization method reduces the total number of fingerprint reference points over the localization space, thus minimizing both the time required for reading radio frequency signals and the number of reference points needed during the fingerprinting learning process, which eventually makes the process less time-consuming. The proposed positioning has two major steps of operation. In the first step, we have realized fingerprinting that utilizes lightly populated reference points (RPs and WCL individually. Using the location estimated at the first step, WCL is run again for the final location estimation. The proposed localization technique reduces the number of required fingerprint RPs by more than 40% compared to normal fingerprinting localization method with a similar localization estimation error.

  14. Molecular beacon biosensors for DNA/RNA analysis

    Science.gov (United States)

    Fang, Xiaohong; Schuster, Sheldon; Liu, Xiaojing; Correll, Tiffany; Zhang, Peng; Tapec, Ruby; Santra, Swadeshmukul; Qhobosheanne, Monde; Lou, Jane H.; Tan, Weihong

    2000-03-01

    We have developed a variety of novel DNA biosensors using a new class of oligonucleotide probe, molecular beacon (MB). MB has the fluorescence signal transduction mechanism built within the molecules. It can report the presence of specific nucleic acids with high sensitivity and excellent selectivity. Biotinylated MBs have been designed and synthesized for immobilization onto silica surface through avidin-biotin binding. The effect of the avidin-biotin bridge on the MB hybridization has been studied. Our result shows that using streptavidin has less effect than using avidin in MB hybridization. Two kinds of fiber optical DNA sensors have been prepared and characterized: a fiber optic evanescent wave sensor and a submicrometer optical fiber sensor. The sensors are rapid,stable, highly selective, reproducible and regenerable. They have been applied to detect specific DNA and mRNA sequences and to the study of the DNA hybridization kinetics. Silica nanoparticles have also been used for MB immobilization in order to prepare a large quantity of nanometer sized DNA/RNA biosensors.

  15. Analytical performance of molecular beacons on surface immobilized gold nanoparticles of varying size and density.

    Science.gov (United States)

    Uddayasankar, Uvaraj; Krull, Ulrich J

    2013-11-25

    The high quenching efficiency of metal nanoparticles has facilitated its use as quenchers in molecular beacons. To optimize this system, a good understanding of the many factors that influence molecular beacon performance is required. In this study, molecular beacon performance was evaluated as a function of gold nanoparticle size and its immobilization characteristics. Gold nanoparticles of 4 nm, 15 nm and 87 nm diameter, were immobilized onto glass slides. Each size regime offered distinctive optical properties for fluorescence quenching of molecular dyes that were conjugated to oligonucleotides that were immobilized to the gold nanoparticles. Rigid double stranded DNA was used as a model to place fluorophores at different distances from the gold nanoparticles. The effect of particle size and also the immobilization density of nanoparticles was evaluated. The 4 nm and 87 nm gold nanoparticles offered the highest sensitivity in terms of the change in fluorescence intensity as a function of distance (3-fold improvement for Cy5). The optical properties of the molecular fluorophore was of significance, with Cy5 offering higher contrast ratios than Cy3 due to the red-shifted emission spectrum relative to the plasmon peak. A high density of gold nanoparticles reduced contrast ratios, indicating preference for a monolayer of immobilized nanoparticles when considering analytical performance. Molecular beacon probes were then used in place of the double stranded oligonucleotides. There was a strong dependence of molecular beacon performance on the length of a linker used for attachment to the nanoparticle surface. The optimal optical performance was obtained with 4 nm gold nanoparticles that were immobilized as monolayers of low density (5.7×10(11)particles cm(-2)) on glass surfaces. These nanoparticle surfaces offered a 2-fold improvement in analytical performance of the molecular beacons when compared to other nanoparticle sizes investigated. The principles developed

  16. BBUNS: Bluetooth Beacon-Based Underground Navigation System to Support Mine Haulage Operations

    Directory of Open Access Journals (Sweden)

    Jieun Baek

    2017-11-01

    Full Text Available A Bluetooth beacon-based underground navigation system (BBUNS was developed to identify the optimal haul road in an underground mine, track the locations of dump trucks, and display this information on mobile devices. A three-dimensional (3-D geographic information system (GIS database of the haul roads in an underground mine was constructed, and the travel time for each section was calculated. A GIS database was also constructed for 50 Bluetooth beacons that were installed along the haul roads. An Android-based BBUNS application was developed to visualize the current location of each dump truck and the optimal haul road to the destination on mobile devices, using the Bluetooth beacon system that was installed in the underground mine. Whenever the BBUNS recognized all of the Bluetooth beacons installed in the underground mine, it could provide the dump truck drivers with information on the current location and the two-dimensional (2-D and 3-D haul road properties. The operating time of each dump truck and the time spent on each unit task could be analyzed using recorded data on the times when Bluetooth beacon signals were recognized by the BBUNS. The underground mine navigation system that was developed in this study can contribute to the improvement of haul operation efficiency and productivity.

  17. Adaptive Beaconing in Mobility Aware Clustering Based MAC Protocol for Safety Message Dissemination in VANET

    Directory of Open Access Journals (Sweden)

    Nishu Gupta

    2017-01-01

    Full Text Available Majority of research contributions in wireless access in vehicular environment (WAVE/IEEE 802.11p standard focus on life critical safety-related applications. These applications require regular status update of vehicle’s position referred to as beaconing. Periodic beaconing in vehicle to vehicle communication leads to severe network congestion in the communication channel. The condition worsens under high vehicular density where it impacts reliability and upper bound latency of safety messages. In this paper, WAVE compliant enhancement to the existing IEEE 802.11p protocol is presented which targets prioritized delivery of safety messages while simultaneously provisioning the dissemination of nonsafety messages. Proposed scheme relies on dynamic generation of beacons to mitigate channel congestion and inefficient bandwidth utilization by reducing transmission frequency of beacons. Through the use of clustering mechanism, different beaconing frequencies and different data transmission rates are assigned to prioritize vehicular mobility. Through extensive simulation results, the performance of the proposed approach is evaluated in terms of a wide range of quality of service (QoS parameters for two different transmission ranges. Results show that the proposed protocol provides significant enhancement and stability of the clustered topology in vehicular ad hoc network over existing standard and other protocols with similar applications.

  18. Molecular beacon anchored onto a graphene oxide substrate

    Science.gov (United States)

    Darbandi, Arash; Datta, Debopam; Patel, Krunal; Lin, Gary; Stroscio, Michael A.; Dutta, Mitra

    2017-09-01

    In this article, we report a graphene oxide-based nanosensor incorporating semiconductor quantum dots linked to DNA-aptamers that functions as a ‘turn-off’ fluorescent nanosensor for detection of low concentrations of analytes. A specific demonstration of this turn-off aptasensor is presented for the case of the detection of mercury (II) ions. In this system, ensembles of aptamer-based quantum-dot sensors are anchored onto graphene oxide (GO) flakes which provide a platform for analyte detection in the vicinity of GO. Herein, the operation of this ensemble-based nanosensor is demonstrated for mercury ions, which upon addition of mercury, quenching of the emission intensity from the quantum dots is observed due to resonance energy transfer between quantum dots and the gold nanoparticle connected via a mercury target aptamer. A key result is that the usually dominant effect of quenching of the quantum dot due to close proximity to the GO can be reduced to negligible levels by using a linker molecule in conjunctions with the aptamer-based nanosensor. The effect of ionic concentration of the background matrix on the emission intensity was also investigated. The sensor system is found to be highly selective towards mercury and exhibits a linear behavior (r 2 > 0.99) in the nanomolar concentration range. The detection limit of the sensor towards mercury with no GO present was found to be 16.5 nM. With GO attached to molecular beacon via 14 base, 35 base, and 51 base long linker DNA, the detection limit was found to be 38.4 nM, 9.45 nM, and 11.38 nM; respectively.

  19. Perlecan domain V therapy for stroke: a beacon of hope?

    Science.gov (United States)

    Bix, Gregory J

    2013-03-20

    The sad reality is that in the year 2012, people are still dying or suffering from the extreme morbidity of ischemic stroke. This tragedy is only compounded by the graveyard full of once promising new therapies. While it is indeed true that the overall mortality from stroke has declined in the United States, perhaps due to increased awareness of stroke symptoms by both the lay public and physicians, it is clear that better therapies are needed. In this regard, progress has been tremendously slowed by the simple fact that experimental models of stroke and the animals that they typically employ, rats and mice, do not adequately represent human stroke. Furthermore, the neuroprotective therapeutic approach, in which potential treatments are administered with the hope of preventing the spread of dying neurons that accompanies a stroke, typically fail for a number of reasons such as there is simply more brain matter to protect in a human than there is in a rodent! For this reason, there has been somewhat of a shift in stroke research away from neuroprotection and toward a neurorepair approach. This too may be problematic in that agents that might foster brain repair could be acutely deleterious or neurotoxic and vice versa, making the timing of treatment administration after stroke critical. Therefore, in our efforts to discover a new stroke therapy, we decided to focus on identifying brain repair elements that were (1) endogenously and actively generated in response to stroke in both human and experimental animal brains, (2) present acutely and chronically after ischemic stroke, suggesting that they could have a role in acute neuroprotection and chronic neurorepair, and (3) able to be administered peripherally and reach the site of stroke brain injury. In this review, I will discuss the evidence that suggests that perlecan domain V may be just that substance, a potential beacon of hope for stroke patients.

  20. A Measurement Study of BLE iBeacon and Geometric Adjustment Scheme for Indoor Location-Based Mobile Applications

    Directory of Open Access Journals (Sweden)

    Jeongyeup Paek

    2016-01-01

    Full Text Available Bluetooth Low Energy (BLE and the iBeacons have recently gained large interest for enabling various proximity-based application services. Given the ubiquitously deployed nature of Bluetooth devices including mobile smartphones, using BLE and iBeacon technologies seemed to be a promising future to come. This work started off with the belief that this was true: iBeacons could provide us with the accuracy in proximity and distance estimation to enable and simplify the development of many previously difficult applications. However, our empirical studies with three different iBeacon devices from various vendors and two types of smartphone platforms prove that this is not the case. Signal strength readings vary significantly over different iBeacon vendors, mobile platforms, environmental or deployment factors, and usage scenarios. This variability in signal strength naturally complicates the process of extracting an accurate location/proximity estimation in real environments. Our lessons on the limitations of iBeacon technique lead us to design a simple class attendance checking application by performing a simple form of geometric adjustments to compensate for the natural variations in beacon signal strength readings. We believe that the negative observations made in this work can provide future researchers with a reference on how well of a performance to expect from iBeacon devices as they enter their system design phases.

  1. Efficient Beacon Collision Resolution Procedure for IEEE 802.15.4 /Zigbee Wireless Personal Area Networks

    Directory of Open Access Journals (Sweden)

    Bassam A. Zafar

    2017-04-01

    Full Text Available While IEEE 802.15.4/Zigbee is a promising technology for Wireless Personal Area Networks, several transmission problems are not yet resolved. In particular, the problem of beacon transmission interferences is causing the device connection loss to the network. In order to resolve this problem, we present a new distributed and reactive procedure for beacon collision resolution. It is an extension of the alignment procedure to reorganize randomly the beacon transmission time when a collision has occurred. The detail of the proposed procedure will be fully described and analyzed. The performance of our approach is performed by simulations. The results show that our approach reduces the collision probability and the device disconnections consequently.

  2. Preliminary Observations of Ionospheric Structures using Ground Receivers and the COSMIC Radio Beacon

    Science.gov (United States)

    Garner, T. W.; Gaussiran, T. L., II; Bust, G. S.

    2006-11-01

    With the launch of the Constellation Observing System for Meteorology, Ionosphere and Climate (COSMIC) satellite constellation, a new era has begun for low-Earth-orbiting (LEO) satellite beacon research has begun. Each COSMIC satellite carries a radio beacon that can emit at 1066, 400, and 150 MHz. Coherent Ionospheric Doppler Receivers (CIDR) deployed at mid-latitudes observe the electron content and phase scintillation in the 400 and 150 MHz channels. In addition to high (Alaska and Greenland) and low (Peru) latitude systems, CIDRs are deployed in Austin, Texas, a chain across southern New York, Millstone Hill, and Wallops Island. This study presents preliminary observations of different ionospheric structures using CIDR receivers and the COSMIC radio beacon.

  3. Time-Resolved Nucleic Acid Hybridization Beacons Utilizing Unimolecular and Toehold-Mediated Strand Displacement Designs.

    Science.gov (United States)

    Massey, Melissa; Ancona, Mario G; Medintz, Igor L; Algar, W Russ

    2015-12-01

    Nucleic acid hybridization probes are sought after for numerous assay and imaging applications. These probes are often limited by the properties of fluorescent dyes, prompting the development of new probes where dyes are paired with novel or nontraditional luminescent materials. Luminescent terbium complexes are an example of such a material, and these complexes offer several unique spectroscopic advantages. Here, we demonstrate two nonstem-loop designs for light-up nucleic acid hybridization beacons that utilize time-resolved Förster resonance energy transfer (TR-FRET) between a luminescent Lumi4-Tb cryptate (Tb) donor and a fluorescent reporter dye, where time-resolved emission from the dye provides an analytical signal. Both designs are based on probe oligonucleotides that are labeled at their opposite termini with Tb and a fluorescent reporter dye. In one design, a probe is partially blocked with a quencher dye-labeled oligonucleotide, and target hybridization is signaled through toehold-mediated strand displacement and loss of a competitive FRET pathway. In the other design, the intrinsic folding properties of an unblocked probe are utilized in combination with a temporal mechanism for signaling target hybridization. This temporal mechanism is based on a recently elucidated "sweet spot" for TR-FRET measurements and exploits distance control over FRET efficiencies to shift the Tb lifetime within or outside the time-gated detection window for measurements. Both the blocked and unblocked beacons offer nanomolar (femtomole) detection limits, response times on the order of minutes, multiplexing through the use of different reporter dyes, and detection in complex matrices such as serum and blood. The blocked beacons offer better mismatch selectivity, whereas the unblocked beacons are simpler in design. The temporal mechanism of signaling utilized with the unblocked beacons also plays a significant role with the blocked beacons and represents a new and effective

  4. Results from the northern New Mexico satellite-beacon radio interferometer

    International Nuclear Information System (INIS)

    Carlos, R.; Jacobson, A.; Massey, R.; Wu, G.

    1994-01-01

    An interferometer described in the Boston, 1992, meeting of the Beacon Satellite Symposium has been in full operation for over a year now. It consists of four autonomous stations; three are in a triangle 70 km on a side and one is in the center. The stations receive the VHF beacons from two geosynchronous satellites, GOES-2 and ATS-3. The phases of the beacons are tracked at each station by referring them to an extremely stable rubidium oscillator. The studies of the two satellites are virtually separate experiments. The received phase of the beacon is retarded by the increased Total-Electron-Content of the dense regions of waves in the ionosphere. By comparing the phase history at four spatially separated stations, the authors can determine the two-dimensional propagation vector of the waves. This array is optimal for wavelengths of 70--300 km (periods of 300--3,000 seconds). Since the measurement is of the phase of the signal rather than the difference between the O-mode and X-mode phases, and since the beacons are in the VHF rather than in the L-band of GPS beacons, the array is very sensitive. It has a noise level of 10 13 electrons/m 2 , or 10 -4 of the normal daytime TEC. This has been verified by operating two stations in the same location, so that they saw the same ionosphere. The first interesting results from a year's study is that the authors do not see the same TID's when looking at the two satellites. One conclusion they draw is that they do not see evidence of ionospheric winds

  5. Theoretical analysis and experimental study of constraint boundary conditions for acquiring the beacon in satellite-ground laser communications

    Science.gov (United States)

    Yu, Siyuan; Wu, Feng; Wang, Qiang; Tan, Liying; Ma, Jing

    2017-11-01

    Acquisition and recognition for the beacon is the core technology of establishing the satellite optical link. In order to acquire the beacon correctly, the beacon image should be recognized firstly, excluding the influence of the background light. In this processing, many factors will influence the recognition precision of the beacon. This paper studies the constraint boundary conditions for acquiring the beacon from the perspective of theory and experiment, and as satellite-ground laser communications, an approach for obtaining the adaptive segmentation method is also proposed. Finally, the long distance laser communication experiment (11.16 km) verifies the validity of this method and the tracking error with the method is the least compared with the traditional approaches. The method helps to greatly improve the tracking precision in the satellite-ground laser communications.

  6. Geologic map of the Beacon Rock quadrangle, Skamania County, Washington

    Science.gov (United States)

    Evarts, Russell C.; Fleck, Robert J.

    2017-06-06

    The Beacon Rock 7.5′ quadrangle is located approximately 50 km east of Portland, Oregon, on the north side of the Columbia River Gorge, a scenic canyon carved through the axis of the Cascade Range by the Columbia River. Although approximately 75,000 people live within the gorge, much of the region remains little developed and is encompassed by the 292,500-acre Columbia River Gorge National Scenic Area, managed by a consortium of government agencies “to pro­tect and provide for the enhancement of the scenic, cultural, recreational and natural resources of the Gorge and to protect and support the economy of the Columbia River Gorge area.” As the only low-elevation corridor through the Cascade Range, the gorge is a critical regional transportation and utilities corridor (Wang and Chaker, 2004). Major state and national highways and rail lines run along both shores of the Columbia River, which also provides important water access to ports in the agricultural interior of the Pacific Northwest. Transmission lines carry power from hydroelectric facilities in the gorge and farther east to the growing urban areas of western Oregon and Washington, and natural-gas pipelines transect the corridor (Wang and Chaker, 2004). These lifelines are highly vulnerable to disruption by earthquakes, landslides, and floods. A major purpose of the work described here is to identify and map geologic hazards, such as faults and landslide-prone areas, to provide more accurate assessments of the risks associated with these features.The steep canyon walls of the map area reveal exten­sive outcrops of Miocene flood-basalt flows of the Columbia River Basalt Group capped by fluvial deposits of the ances­tral Columbia River, Pliocene lavas erupted from the axis of the Cascade arc to the east, and volcanic rocks erupted from numerous local vents. The Columbia River Basalt Group unconformably rests on a sequence of late Oligocene and early Miocene rocks of the ancestral Cascade volcanic arc

  7. Toxoplasma gondii DNA detection with a magnetic molecular beacon probe

    Science.gov (United States)

    Xu, Shichao; Yao, Cuicui; Wei, Shuoming; Zhang, Jimei; Dai, Zhao; Zheng, Guo; Sun, Bo; Han, Qing; Hu, Fei; Zhou, Hongming

    2008-12-01

    Toxoplasma Gondii infection is widespread in humans worldwide and reported infection rates range from 3%-70%, depending on the populations or geographic areas, and it has been recognized as a potential food safety hazard in our daily life. A magnetic molecular beacon probe (mMBP), based on theory of fluorescence resonance energy transfer (FRET), was currently reported to detect Toxoplasma Gondii DNA. Nano-sized Fe3O4 were primarily prepared by coprecipitation method in aqueous phase with NaOH as precipitator, and was used as magnetic core. The qualified coreshell magnetic quantum dots (mQDs), i.e. CdTe(symbol)Fe3O4, were then achieved by layer-by-layer method when mol ratio of Fe3O4/CdTe is 1/3, pH at 6.0, 30 °C, and reactant solution was refluxed for 30 min, the size of mQDs were determined to be 12-15 nm via transmission electron microscopy (TEM). Over 70% overlap between emission spectrum of mQDs and absorbance spectrum of BHQ-2 was observed, this result suggests the synthesized mQDs and BHQ-2 can be utilized as energy donor and energy acceptor, respectively. The sensing probe was fabricated and a stem-loop Toxoplasma Gondii DNA oligonucleotide was labeled with mQDs at the 5' end and BHQ-2 at 3' end, respectively. Target Toxoplasma gondii DNA was detected under conditions of 37 °C, hybridization for 2h, at pH8.0 in Tris-HCl buffer. About 30% recovery of fluorescence intensity was observed via fluorescence spectrum (FS) after the Toxoplasma gondii DNA was added, which suggested that the Toxoplasma Gondii DNA was successfully detected. Specificity investigation of the mMBP indicated that relative low recovery of fluorescence intensity was obtained when the target DNA with one-base pair mismatch was added, this result indicated the high specificity of the sensing probe. Our research simultaneously indicated that mMBP can be conveniently separated from the unhybridized stem-loop DNA and target DNA, which will be meaningful in DNA sensing and purification process.

  8. Photodynamic Molecular Beacons: An Image-Guided Therapeutic Approach to Breast Cancer Vertebral Metastases

    Science.gov (United States)

    2012-03-01

    spinal metastases are predominantly osteolytic (bone destructive), which is associated with an increase in osteo- clast activity.1 Metastasis disrupts...0.001). The distributions and shape of the cells are largely homogeneous after incubation with the beacons, suggesting minimal toxicity (SI Figure S3

  9. The Effects of a Rectangular Rapid-Flashing Beacon on Vehicle Speed

    Science.gov (United States)

    VanWagner, Michelle; Van Houten, Ron; Betts, Brian

    2011-01-01

    In 2008, nearly 31% of vehicle fatalities were related to failure to adhere to safe vehicle speeds (National Highway Traffic Safety Administration [NHTSA], 2009). The current study evaluated the effect of a rectangular rapid-flashing beacon (RRFB) triggered by excessive speed on vehicle speed using a combined alternating treatments and reversal…

  10. A Beacon Transmission Power Control Algorithm Based on Wireless Channel Load Forecasting in VANETs.

    Science.gov (United States)

    Mo, Yuanfu; Yu, Dexin; Song, Jun; Zheng, Kun; Guo, Yajuan

    2015-01-01

    In a vehicular ad hoc network (VANET), the periodic exchange of single-hop status information broadcasts (beacon frames) produces channel loading, which causes channel congestion and induces information conflict problems. To guarantee fairness in beacon transmissions from each node and maximum network connectivity, adjustment of the beacon transmission power is an effective method for reducing and preventing channel congestion. In this study, the primary factors that influence wireless channel loading are selected to construct the KF-BCLF, which is a channel load forecasting algorithm based on a recursive Kalman filter and employs multiple regression equation. By pre-adjusting the transmission power based on the forecasted channel load, the channel load was kept within a predefined range; therefore, channel congestion was prevented. Based on this method, the CLF-BTPC, which is a transmission power control algorithm, is proposed. To verify KF-BCLF algorithm, a traffic survey method that involved the collection of floating car data along a major traffic road in Changchun City is employed. By comparing this forecast with the measured channel loads, the proposed KF-BCLF algorithm was proven to be effective. In addition, the CLF-BTPC algorithm is verified by simulating a section of eight-lane highway and a signal-controlled urban intersection. The results of the two verification process indicate that this distributed CLF-BTPC algorithm can effectively control channel load, prevent channel congestion, and enhance the stability and robustness of wireless beacon transmission in a vehicular network.

  11. A Beacon Transmission Power Control Algorithm Based on Wireless Channel Load Forecasting in VANETs.

    Directory of Open Access Journals (Sweden)

    Yuanfu Mo

    Full Text Available In a vehicular ad hoc network (VANET, the periodic exchange of single-hop status information broadcasts (beacon frames produces channel loading, which causes channel congestion and induces information conflict problems. To guarantee fairness in beacon transmissions from each node and maximum network connectivity, adjustment of the beacon transmission power is an effective method for reducing and preventing channel congestion. In this study, the primary factors that influence wireless channel loading are selected to construct the KF-BCLF, which is a channel load forecasting algorithm based on a recursive Kalman filter and employs multiple regression equation. By pre-adjusting the transmission power based on the forecasted channel load, the channel load was kept within a predefined range; therefore, channel congestion was prevented. Based on this method, the CLF-BTPC, which is a transmission power control algorithm, is proposed. To verify KF-BCLF algorithm, a traffic survey method that involved the collection of floating car data along a major traffic road in Changchun City is employed. By comparing this forecast with the measured channel loads, the proposed KF-BCLF algorithm was proven to be effective. In addition, the CLF-BTPC algorithm is verified by simulating a section of eight-lane highway and a signal-controlled urban intersection. The results of the two verification process indicate that this distributed CLF-BTPC algorithm can effectively control channel load, prevent channel congestion, and enhance the stability and robustness of wireless beacon transmission in a vehicular network.

  12. Scheduled MAC in Beacon Overlay Networks for Underwater Localization and Time-Synchronization

    NARCIS (Netherlands)

    van Kleunen, W.A.P.; Meratnia, Nirvana; Havinga, Paul J.M.

    2011-01-01

    In this article we introduce a MAC protocol designed for underwater localization and time-synchronisation. The MAC protocol assumes a network of static reference nodes and allows blind nodes to be localized by listening-only to the beacon messages. Such a system is known to be very scalable. We show

  13. The Roles of Beaconing and Dead Reckoning in Human Virtual Navigation

    Science.gov (United States)

    Bodily, Kent D.; Daniel, Thomas A.; Sturz, Bradley R.

    2012-01-01

    Beaconing is a process in which the distance between a visual landmark and current position is reduced in order to return to a location. In contrast, dead reckoning is a process in which vestibular, kinesthetic and/or optic flow cues are utilized to update speed of movement, elapsed time of movement, and direction of movement to return to a…

  14. Impacts of Fog Characteristics, Forward Illumination, and Warning Beacon Intensity Distribution on Roadway Hazard Visibility

    Directory of Open Access Journals (Sweden)

    John D. Bullough

    2016-01-01

    Full Text Available Warning beacons are critical for the safety of transportation, construction, and utility workers. These devices need to produce sufficient luminous intensity to be visible without creating glare to drivers. Published standards for the photometric performance of warning beacons do not address their performance in conditions of reduced visibility such as fog. Under such conditions light emitted in directions other than toward approaching drivers can create scattered light that makes workers and other hazards less visible. Simulations of visibility of hazards under varying conditions of fog density, forward vehicle lighting, warning beacon luminous intensity, and intensity distribution were performed to assess their impacts on visual performance by drivers. Each of these factors can influence the ability of drivers to detect and identify workers and hazards along the roadway in work zones. Based on the results, it would be reasonable to specify maximum limits on the luminous intensity of warning beacons in directions that are unlikely to be seen by drivers along the roadway, limits which are not included in published performance specifications.

  15. Beacon-Based Service Publishing Framework in Multiservice Wi-Fi Hotspots

    Directory of Open Access Journals (Sweden)

    Di Sorte Dario

    2007-01-01

    Full Text Available In an expected future multiaccess and multiservice IEEE 802.11 environment, the problem of providing users with useful service-related information to support a correct rapid network selection is expected to become a very important issue. A feasible short-term 802.11-tailored working solution, compliant with existing equipment, is to publish service information encoded within the SSID information element within beacon frames. This makes it possible for an operator to implement service publishing in 802.11 networks while waiting for a standardized mechanism. Also, this straightforward approach has allowed us to evaluate experimentally the performance of a beacon-based service publishing solution. In fact, the main focus of the paper is indeed to present a quantitative comparison of service discovery times between the legacy scenario, where the user is forced to associate and authenticate with a network point of access to check its service offer, and the enhanced scenario where the set of service-related information is broadcasted within beacons. These discovery times are obtained by processing the results of a measurement campaign performed in a multiaccess/service 802.11 environment. This analysis confirms the effectiveness of the beacon-based approach. We also show that the cost in terms of wireless bandwidth consumption of such solution is low.

  16. Analysis and Enhancement of IEEE 802.15.4e DSME Beacon Scheduling Model

    Directory of Open Access Journals (Sweden)

    Kwang-il Hwang

    2014-01-01

    Full Text Available In order to construct a successful Internet of things (IoT, reliable network construction and maintenance in a sensor domain should be supported. However, IEEE 802.15.4, which is the most representative wireless standard for IoT, still has problems in constructing a large-scale sensor network, such as beacon collision. To overcome some problems in IEEE 802.15.4, the 15.4e task group proposed various different modes of operation. Particularly, the IEEE 802.15.4e deterministic and synchronous multichannel extension (DSME mode presents a novel scheduling model to solve beacon collision problems. However, the DSME model specified in the 15.4e draft does not present a concrete design model but a conceptual abstract model. Therefore, in this paper we introduce a DSME beacon scheduling model and present a concrete design model. Furthermore, validity and performance of DSME are evaluated through experiments. Based on experiment results, we analyze the problems and limitations of DSME, present solutions step by step, and finally propose an enhanced DSME beacon scheduling model. Through additional experiments, we prove the performance superiority of enhanced DSME.

  17. Supplementary Material for: BEACON: automated tool for Bacterial GEnome Annotation ComparisON

    KAUST Repository

    Kalkatawi, Manal M.

    2015-01-01

    Abstract Background Genome annotation is one way of summarizing the existing knowledge about genomic characteristics of an organism. There has been an increased interest during the last several decades in computer-based structural and functional genome annotation. Many methods for this purpose have been developed for eukaryotes and prokaryotes. Our study focuses on comparison of functional annotations of prokaryotic genomes. To the best of our knowledge there is no fully automated system for detailed comparison of functional genome annotations generated by different annotation methods (AMs). Results The presence of many AMs and development of new ones introduce needs to: a/ compare different annotations for a single genome, and b/ generate annotation by combining individual ones. To address these issues we developed an Automated Tool for Bacterial GEnome Annotation ComparisON (BEACON) that benefits both AM developers and annotation analysers. BEACON provides detailed comparison of gene function annotations of prokaryotic genomes obtained by different AMs and generates extended annotations through combination of individual ones. For the illustration of BEACONâ s utility, we provide a comparison analysis of multiple different annotations generated for four genomes and show on these examples that the extended annotation can increase the number of genes annotated by putative functions up to 27 %, while the number of genes without any function assignment is reduced. Conclusions We developed BEACON, a fast tool for an automated and a systematic comparison of different annotations of single genomes. The extended annotation assigns putative functions to many genes with unknown functions. BEACON is available under GNU General Public License version 3.0 and is accessible at: http://www.cbrc.kaust.edu.sa/BEACON/ .

  18. Design of a K/Q-Band Beacon Receiver for the Alphasat TDP#5 Experiment

    Science.gov (United States)

    Morse, Jacquelynne R.

    2014-01-01

    This paper describes the design and performance of a coherent KQ-band (2040 GHz) beacon receiver developed at NASA Glenn Research Center (GRC) that will be installed at the Politecnico di Milano (POLIMI) for use in the Alphasat Technology Demonstration Payload 5 (TDP5) beacon experiment. The goal of this experiment is to characterize rain fade attenuation at 40 GHz to improve the performance of existing statistical rain attenuation models in the Q-band. The ground terminal developed by NASA GRC utilizes an FFT-based frequency estimation receiver capable of characterizing total path attenuation effects due to gaseous absorption, clouds, rain, and scintillation. The receiver system has been characterized in the lab and demonstrates a system dynamic range performance of better than 58 dB at 1 Hz and better than 48 dB at 10 Hz rates.

  19. Multi-Band Multi-Tone Tunable Millimeter-Wave Frequency Synthesizer For Satellite Beacon Transmitter

    Science.gov (United States)

    Simons, Rainee N.; Wintucky, Edwin G.

    2016-01-01

    This paper presents the design and test results of a multi-band multi-tone tunable millimeter-wave frequency synthesizer, based on a solid-state frequency comb generator. The intended application of the synthesizer is in a satellite beacon transmitter for radio wave propagation studies at K-band (18 to 26.5 GHz), Q-band (37 to 42 GHz), and E-band (71 to 76 GHz). In addition, the architecture for a compact beacon transmitter, which includes the multi-tone synthesizer, polarizer, horn antenna, and power/control electronics, has been investigated for a notional space-to-ground radio wave propagation experiment payload on a small satellite. The above studies would enable the design of robust high throughput multi-Gbps data rate future space-to-ground satellite communication links.

  20. Creation of a Library Tour Application for Mobile Equipment using iBeacon Technology

    Directory of Open Access Journals (Sweden)

    Jonathan Bradley

    2016-04-01

    Full Text Available We describe the design, development, and deployment of a library tour application utilizing Bluetooth Low Energy devices know as iBeacons. The tour application will serve as library orientation for incoming students. The students visit stations in the library with mobile equipment running a special tour app. When the app detects a beacon nearby, it automatically plays a video that describes the current location. After the tour, students are assessed according to the defined learning objectives. Special attention is given to issues encountered during development, deployment, content creation, and testing of this application that depend on functioning hardware, and the necessity of appointing a project manager to limit scope, define priorities, and create an actionable plan for the experiment.

  1. Nanosecond-level time synchronization of AERA using a beacon reference transmitter and commercial airplanes

    Science.gov (United States)

    Huege, Tim

    2017-03-01

    Radio detection of cosmic-ray air showers requires time synchronization of detectors on a nanosecond level, especially for advanced reconstruction algorithms based on the wavefront curvature and for interferometric analysis approaches. At the Auger Engineering Radio Array, the distributed, autonomous detector stations are time-synchronized via the Global Positioning System which, however, does not provide sufficient timing accuracy. We thus employ a dedicated beacon reference transmitter to correct for eventby-event clock drifts in our offline data analysis. In an independent cross-check of this "beacon correction" using radio pulses emitted by commercial airplanes, we have shown that the combined timing accuracy of the two methods is better than 2 nanoseconds.

  2. Nanosecond-level time synchronization of AERA using a beacon reference transmitter and commercial airplanes

    Directory of Open Access Journals (Sweden)

    Huege Tim

    2017-01-01

    Full Text Available Radio detection of cosmic-ray air showers requires time synchronization of detectors on a nanosecond level, especially for advanced reconstruction algorithms based on the wavefront curvature and for interferometric analysis approaches. At the Auger Engineering Radio Array, the distributed, autonomous detector stations are time-synchronized via the Global Positioning System which, however, does not provide sufficient timing accuracy. We thus employ a dedicated beacon reference transmitter to correct for eventby-event clock drifts in our offline data analysis. In an independent cross-check of this “beacon correction” using radio pulses emitted by commercial airplanes, we have shown that the combined timing accuracy of the two methods is better than 2 nanoseconds.

  3. Surface-attached molecular beacons light the way for DNA sequencing

    Czech Academy of Sciences Publication Activity Database

    Paleček, Emil

    2004-01-01

    Roč. 22, č. 2 (2004), s. 55-58 ISSN 0167-7799 R&D Projects: GA AV ČR IBS5004355; GA ČR GA204/03/0566 Institutional research plan: CEZ:AV0Z5004920 Keywords : molecular beacon * DNA stem-loop structure * DNA sensors Subject RIV: BO - Biophysics Impact factor: 8.606, year: 2004

  4. Beaconing Performance in IEEE 802.11p Vehicular Networks: the Effect of Radio Channel Congestion

    OpenAIRE

    Librino, Francesco; Renda, Maria Elena; Santi, Paolo

    2013-01-01

    In this paper, we study the performance of the beaconing mechanism underlying active safety vehicular applications in presence of different levels of channel congestion. The importance of this study lies in the fact that channel congestion is considered a major factor influencing communication performance in vehicular networks, and that ours is the first investigation of the effects of congestion based on extensive, real-world measurements. The results of our study reveal that congestion has ...

  5. Beacon: A three-dimensional structural analysis code for bowing history of fast breeder reactor cores

    International Nuclear Information System (INIS)

    Miki, K.

    1979-01-01

    The core elements of an LMFBR are bowed due to radial gradients of both temperature and neutron flux in the core. Since all hexagonal elements are multiply supported by adjacent elements or the restraint system, restraint forces and bending stresses are induced. In turn, these forces and stresses are relaxed by irradiation enhanced creep of the material. The analysis of the core bowing behavior requires a three-dimensional consideration of the mechanical interactions among the core elements, because the core consists of different kinds of elements and of fuel assemblies with various burnup histories. A new computational code BEACON has been developed for analyzing the bowing behavior of an LMFBR's core in three dimensions. To evaluate mechanical interactions among core elements, the code uses the analytical method of the earlier SHADOW code. BEACON analyzes the mechanical interactions in three directions, which form angles of 60 0 with one another. BEACON is applied to the 60 0 sector of a typical LMFBR's core for analyzing the bowing history during one equilibrium cycle. 120 core elements are treated, assuming the boundary condition of rotational symmetry. The application confirms that the code can be an effective tool for parametric studies as well as for detailed structural analysis of LMFBR's core. (orig.)

  6. Sparse Localization with a Mobile Beacon Based on LU Decomposition in Wireless Sensor Networks

    Directory of Open Access Journals (Sweden)

    Chunhui Zhao

    2015-09-01

    Full Text Available Node localization is the core in wireless sensor network. It can be solved by powerful beacons, which are equipped with global positioning system devices to know their location information. In this article, we present a novel sparse localization approach with a mobile beacon based on LU decomposition. Our scheme firstly translates node localization problem into a 1-sparse vector recovery problem by establishing sparse localization model. Then, LU decomposition pre-processing is adopted to solve the problem that measurement matrix does not meet the re¬stricted isometry property. Later, the 1-sparse vector can be exactly recovered by compressive sensing. Finally, as the 1-sparse vector is approximate sparse, weighted Cen¬troid scheme is introduced to accurately locate the node. Simulation and analysis show that our scheme has better localization performance and lower requirement for the mobile beacon than MAP+GC, MAP-M, and MAP-MN schemes. In addition, the obstacles and DOI have little effect on the novel scheme, and it has great localization performance under low SNR, thus, the scheme proposed is robust.

  7. A Microfluidic Love-Wave Biosensing Device for PSA Detection Based on an Aptamer Beacon Probe.

    Science.gov (United States)

    Zhang, Feng; Li, Shuangming; Cao, Kang; Wang, Pengjuan; Su, Yan; Zhu, Xinhua; Wan, Ying

    2015-06-11

    A label-free and selective aptamer beacon-based Love-wave biosensing device was developed for prostate specific antigen (PSA) detection. The device consists of the following parts: LiTaO3 substrate with SiO2 film as wave guide layer, two set of inter-digital transducers (IDT), gold film for immobilization of the biorecongniton layer and a polydimethylsiloxane (PDMS) microfluidic channels. DNA aptamer, or "artificial antibody", was used as the specific biorecognition probe for PSA capture. Some nucleotides were added to the 3'-end of the aptamer to form a duplex with the 3'-end, turning the aptamer into an aptamer-beacon. Taking advantage of the selective target-induced assembly changes arising from the "aptamer beacon", highly selective and specific detection of PSA was achieved. Furthermore, PDMS microfluidic channels were designed and fabricated to realize automated quantitative sample injection. After optimization of the experimental conditions, the established device showed good performance for PSA detection between 10 ng/mL to 1 μg/mL, with a detection limit of 10 ng/mL. The proposed sensor might be a promising alternative for point of care diagnostics.

  8. A novel strategy for human papillomavirus detection and genotyping with SybrGreen and molecular beacon polymerase chain reaction.

    Science.gov (United States)

    Szuhai, K; Sandhaus, E; Kolkman-Uljee, S M; Lemaître, M; Truffert, J C; Dirks, R W; Tanke, H J; Fleuren, G J; Schuuring, E; Raap, A K

    2001-11-01

    Human papillomaviruses (HPVs) play an important role in the pathogenesis of cervical cancer. For identification of the large number of different HPV types found in (pre)malignant lesions, a robust methodology is needed that combines general HPV detection with HPV genotyping. We have developed for formaldehyde-fixed samples a strategy that, in a homogeneous, real-time fluorescence polymerase chain reaction (PCR)-based assay, accomplishes general HPV detection by SybrGreen reporting of HPV-DNA amplicons, and genotyping of seven prevalent HPV types (HPV-6, -11, -16, -18, -31, -33, -45) by real-time molecular beacon PCR. The false-positive rate of the HPV SybrGreen-PCR was 4%, making it well suited as a prescreening, general HPV detection technology. The type specificity of the seven selected HPV molecular beacons was 100% and double infections were readily identified. The multiplexing capacity of the HPV molecular beacon PCR was analyzed and up to three differently labeled molecular beacons could be used in one PCR reaction without observing cross talk. The inherent quantitation capacities of real-time fluorescence PCR allowed the determination of average HPV copy number per cell. We conclude that the HPV SybrGreen-PCR in combination with the HPV molecular beacon PCR provides a robust, sensitive, and quantitative general HPV detection and genotyping methodology.

  9. Non-linear molecular pattern classification using molecular beacons with multiple targets.

    Science.gov (United States)

    Lee, In-Hee; Lee, Seung Hwan; Park, Tai Hyun; Zhang, Byoung-Tak

    2013-12-01

    In vitro pattern classification has been highlighted as an important future application of DNA computing. Previous work has demonstrated the feasibility of linear classifiers using DNA-based molecular computing. However, complex tasks require non-linear classification capability. Here we design a molecular beacon that can interact with multiple targets and experimentally shows that its fluorescent signals form a complex radial-basis function, enabling it to be used as a building block for non-linear molecular classification in vitro. The proposed method was successfully applied to solving artificial and real-world classification problems: XOR and microRNA expression patterns. Copyright © 2013 Elsevier Ireland Ltd. All rights reserved.

  10. Enzymatic Amplification of DNA/RNA Hybrid Molecular Beacon Signaling in Nucleic Acid Detection

    OpenAIRE

    Jacroux, Thomas; Rieck, Daniel C.; Cui, Rong; Ouyang, Yexin; Dong, Wen-Ji

    2012-01-01

    A rapid assay operable under isothermal or non-isothermal conditions is described wherein the sensitivity of a typical molecular beacon (MB) system is improved by utilizing thermostable RNase H to enzymatically cleave an MB comprised of a DNA stem and RNA loop (R/D-MB). Upon hybridization of the R/D-MB to target DNA, there was a modest increase in fluorescence intensity (~5.7x above background) due to an opening of the probe and concomitant reduction in the Förster resonance energy transfer e...

  11. Decentralized Single-beacon Acoustic Navigation: Combined Communication and Navigation for Underwater Vehicles

    Science.gov (United States)

    2010-06-01

    navigation beacons. When the modem is in synchronous navigation ( SNV ) mode, as described in [39], the modem’s clock can be synchronized to a PPS signal using...In SNV mode, all transmitted messages are initiated by the modem within ± 10 µs of the rising edge of the PPS signal [39]. 3.3.2 PPSBoard The PPSBoard...reference for use subsea that can be synchronized to a GPS timing signal. In addition to supplying a PPS signal to the Micro- Modem to enable its SNV

  12. Optical Measurement of a Compressible Shear Layer Using a Laser-Induced Air Breakdown Beacon (Preprint)

    Science.gov (United States)

    2009-12-01

    Comrol Unil’ersity of NOire Dame. Notre Dame. IN. 46556 David Goorskei, Matthew R. Whiteley4 MZA Associates Corporation, Dayton. OH. 45459 David...Cavalieri s, and Eric J. Jumper6 Cenler for Flow Physics and Comrol University of NOire Dame. Noire Dame. IN. 46556 The aero-optic aberrations due to...wave fronts. th3t limit the ability of the beacon system to detect aero-optic aberrations that ha\\’e an amplitude below the noi5e level. Figure 3

  13. A mobile indoor positioning system based on iBeacon technology.

    Science.gov (United States)

    Lin, Xin-Yu; Ho, Te-Wei; Fang, Cheng-Chung; Yen, Zui-Shen; Yang, Bey-Jing; Lai, Feipei

    2015-01-01

    To increase the efficiency in the emergency room, the goal of this research is to implement a mobile-based indoor positioning system using mobile applications (APP) with the iBeacon solution based on the Bluetooth Low Energy (BLE) technology. We use the Received Signal Strength (RSS) based localization method to estimate the patients' locations. Our positioning algorithm achieves 97.22% (95% Confidence Interval = 95.90% - 98.55%) accuracy of classification. As the result, our mechanism is reliable enough to satisfy the need for medical staff to track the locations of their patients.

  14. The Southeastern Minnesota Beacon Project for Community-driven Health Information Technology: Origins, Achievements, and Legacy.

    Science.gov (United States)

    Chute, Christopher G; Hart, Lacey A; Alexander, Alex K; Jensen, Daniel W

    2014-01-01

    The Southeastern (SE) Minnesota Beacon organized all the health care providers, county public health organizations, and school districts in the deployment and integration of health information exchange (HIE) and targeted health communication around childhood asthma and diabetes. The community cooperated to establish a clinical data repository for all residents in the 11-county region. Through this community of practice approach that involved traditional and nontraditional providers, the SE Minnesota Beacon was able to realize unique applications of this technology. This manuscript overviews the associated organization and infrastructure of this community collaboration. The Office of the National Coordinator for Health Information Technology (ONC), as part of the American Recovery and Reinvestment Act of 2009 (ARRA) stimulus, established 17 projects throughout the United States targeting the introduction and meaningful use of health information technology (HIT). These 17 communities were intended to serve as an example of what could be accomplished. The SE Minnesota Beacon is one of these communities. The community ultimately opted for peer-to-peer HIE, using Nationwide Health Information Network (NwHIN) Connect software. The clinical data repository was established using the infrastructure developed by the Regenstrief Institute, which operated as a trusted third party. As an extension to HIE, the consortium of county public health departments created a patient data portal for use by school nurses and parents. Childhood asthma was addressed by creating, exchanging, and maintaining an "asthma action plan" for each affected child, shared throughout the community, including through the patient portal. Diabetes management introduced patient treatment decision tools and patient quality of life measures, facilitating care. Influenza vaccination was enhanced by large-scale community reporting in partnership with the state vaccination registry. The methodology and

  15. Perancangan Prototipe Receiver Beacon Black Box Locator Acoustic 37,5 kHz Pingers

    Directory of Open Access Journals (Sweden)

    RUSTAMAJI RUSTAMAJI

    2016-02-01

    Full Text Available ABSTRAK Ketika suatu pesawat terbang mengalami kecelakaan terjatuh ke dalam air, maka lokasi keberadaannya dapat dideteksi oleh alat yang disebut receiver beacon black box locator acoustic (pingers receiver. Pingers receiver berfungsi untuk menerima sinyal dengan frekuensi 37,5 kHz ± 1 kHz dari pingers transmitter atau Underwater Locator Beacon (ULB yang berada pada black box pesawat. Dalam penelitian ini dibuat perancangan pingers receiver yang tersusun dari rangkaian Band Pass Filter (BPF, detektor selubung, penguat audio, dan loud speaker. Frekuensi sinyal 37,5 kHz ± 1 kHz yang dikirim oleh pingers transmitter akan diterima oleh pingers receiver, dimana tahap pertama melewati rangkaian Band Pass Filter (BPF selanjutnya sinyal akan dideteksi oleh detektor selubung untuk mengambil kembali sinyal informasi yang berupa sinyal pulsa dengan lebar pulsa 10 milidetik dan interval 1 detik sebagai sinyal pingers yang nantinya mampu didengar oleh telinga manusia. Berdasarkan perancangan yang telah dibuat, output sinyal yang dihasilkan oleh prototipe pingers receiver tersebut memiliki lebar pulsa 14 milidetik dengan interval 1 detik. Kata kunci: pingers receiver, band pass filter (BPF, detektor selubung, loud speaker. ABSTRACT When an aircraft caught in an accident and crashes into the water, its location can be detected using a device called a receiver beacon black box locator acoustic (pingers receiver. The pingers receiver functioned as a reciever signal with 37,5 kHz ± 1 kHz frequency from trnasmitter pingers or Underwater Locator Beacon (ULB which is on air plane black blox. In this research made design pingers receiver composed of a series of  Band Pass Filter (BPF, envelope detector, audio amplifier, and loud speaker. The signal of 37,5 kHz ± 1 kHz  frequency sent by pingers transmitter will bereceived by pingers receiver, after which the signal will be processed by the Band Pass Filter (BPF circuit before being detected by envelope detector

  16. Preliminary Statistics from the NASA Alphasat Beacon Receiver in Milan, Italy

    Science.gov (United States)

    Nessel, James; Zemba, Michael; Morse, Jacquelynne; Luini, Lorenzo; Riva, Carlo

    2015-01-01

    NASA Glenn Research Center (GRC) and the Politecnico di Milano (POLIMI) have initiated a joint propagation campaign within the framework of the Alphasat propagation experiment to characterize rain attenuation, scintillation, and gaseous absorption effects of the atmosphere in the 40 gigahertz band. NASA GRC has developed and installed a K/Q-band (20/40 gigahertz) beacon receiver at the POLIMI campus in Milan, Italy, which receives the 20/40 gigahertz signals broadcast from the Alphasat Aldo Paraboni TDP no. 5 beacon payload. The primary goal of these measurements is to develop a physical model to improve predictions of communications systems performance within the Q-band. Herein, we provide an overview of the design and data calibration procedure, and present 6 months of preliminary statistics of the NASA propagation terminal, which has been installed and operating in Milan since May 2014. The Q-band receiver has demonstrated a dynamic range of 40 decibels at an 8-hertz sampling rate. A weather station with an optical disdrometer is also installed to characterize rain drop size distribution for correlation with physical based models.

  17. Secure Utilization of Beacons and UAVs in Emergency Response Systems for Building Fire Hazard.

    Science.gov (United States)

    Seo, Seung-Hyun; Choi, Jung-In; Song, Jinseok

    2017-09-25

    An intelligent emergency system for hazard monitoring and building evacuation is a very important application area in Internet of Things (IoT) technology. Through the use of smart sensors, such a system can provide more vital and reliable information to first-responders and also reduce the incidents of false alarms. Several smart monitoring and warning systems do already exist, though they exhibit key weaknesses such as a limited monitoring coverage and security, which have not yet been sufficiently addressed. In this paper, we propose a monitoring and emergency response method for buildings by utilizing beacons and Unmanned Aerial Vehicles (UAVs) on an IoT security platform. In order to demonstrate the practicability of our method, we also implement a proof of concept prototype, which we call the UAV-EMOR (UAV-assisted Emergency Monitoring and Response) system. Our UAV-EMOR system provides the following novel features: (1) secure communications between UAVs, smart sensors, the control server and a smartphone app for security managers; (2) enhanced coordination between smart sensors and indoor/outdoor UAVs to expand real-time monitoring coverage; and (3) beacon-aided rescue and building evacuation.

  18. A Two-Layer Method for Sedentary Behaviors Classification Using Smartphone and Bluetooth Beacons.

    Science.gov (United States)

    Cerón, Jesús D; López, Diego M; Hofmann, Christian

    2017-01-01

    Among the factors that outline the health of populations, person's lifestyle is the more important one. This work focuses on the caracterization and prevention of sedentary lifestyles. A sedentary behavior is defined as "any waking behavior characterized by an energy expenditure of 1.5 METs (Metabolic Equivalent) or less while in a sitting or reclining posture". To propose a method for sedentary behaviors classification using a smartphone and Bluetooth beacons considering different types of classification models: personal, hybrid or impersonal. Following the CRISP-DM methodology, a method based on a two-layer approach for the classification of sedentary behaviors is proposed. Using data collected from a smartphones' accelerometer, gyroscope and barometer; the first layer classifies between performing a sedentary behavior and not. The second layer of the method classifies the specific sedentary activity performed using only the smartphone's accelerometer and barometer data, but adding indoor location data, using Bluetooth Low Energy (BLE) beacons. To improve the precision of the classification, both layers implemented the Random Forest algorithm and the personal model. This study presents the first available method for the automatic classification of specific sedentary behaviors. The layered classification approach has the potential to improve processing, memory and energy consumption of mobile devices and wearables used.

  19. A Microfluidic Love-Wave Biosensing Device for PSA Detection Based on an Aptamer Beacon Probe

    Directory of Open Access Journals (Sweden)

    Feng Zhang

    2015-06-01

    Full Text Available A label-free and selective aptamer beacon-based Love-wave biosensing device was developed for prostate specific antigen (PSA detection. The device consists of the following parts: LiTaO3 substrate with SiO2 film as wave guide layer, two set of inter-digital transducers (IDT, gold film for immobilization of the biorecongniton layer and a polydimethylsiloxane (PDMS microfluidic channels. DNA aptamer, or “artificial antibody”, was used as the specific biorecognition probe for PSA capture. Some nucleotides were added to the 3'-end of the aptamer to form a duplex with the 3'-end, turning the aptamer into an aptamer-beacon. Taking advantage of the selective target-induced assembly changes arising from the “aptamer beacon”, highly selective and specific detection of PSA was achieved. Furthermore, PDMS microfluidic channels were designed and fabricated to realize automated quantitative sample injection. After optimization of the experimental conditions, the established device showed good performance for PSA detection between 10 ng/mL to 1 μg/mL, with a detection limit of 10 ng/mL. The proposed sensor might be a promising alternative for point of care diagnostics.

  20. Measurements of Tilt and Focus for Sodium Beacon Adaptive Optics on the Starfire 3.5 Meter Telescope

    Science.gov (United States)

    2010-09-01

    beacon and natural guide star operation. This approach, and a more complicated approach, are described in detail by Link and Foucault [3]. They show these...Astronomical Telescopes and Instrumentation Conference, Glasgow, Scotland, 21–25 June 2004. 3. Link D. and Foucault B., "Investigation of focus control

  1. A quencher-free molecular beacon design based on pyrene excimer fluorescence using pyrene-labeled UNA (unlocked nucleic acid)

    DEFF Research Database (Denmark)

    Karlsen, Kasper Kannegård; Okholm, Anders Hauge; Kjems, Jørgen

    2013-01-01

    A quencher-free molecular beacon capable of generating pyrene excimer fluorescence has been constructed using strategically positioned pyrene-UNA monomers. Hybridization of a fully complementary RNA target was accompanied by a pyrene excimer emission increase of more than 900%, and detection of R...... in living cells was demonstrated....

  2. Impact of IEEE 1609.4 channel switching on the IEEE 802.11p beaconing performance

    NARCIS (Netherlands)

    van Eenennaam, Martijn; van de Venis, Anne; Karagiannis, Georgios

    2012-01-01

    The IEEE 802.11p Wireless Access in Vehicular Environment (WAVE) protocol can only transmit packets on one channel. To support multi-channel operations, channel switching procedures have been proposed in IEEE 1609.4. This paper provides an analysis of the beaconing performance of IEEE 802.11p when

  3. 75 FR 20364 - Public Buildings Service; Key Largo Beacon Annex Site; Key Largo, FL; Transfer of Property

    Science.gov (United States)

    2010-04-19

    ... GENERAL SERVICES ADMINISTRATION [Wildlife Order 187; 4-D-FL-1218] Public Buildings Service; Key Largo Beacon Annex Site; Key Largo, FL; Transfer of Property Pursuant to section 2 of Public Law 537, 80th Congress, approved May 19, 1948 (16 U.S.C. 667c), notice is hereby given that: 1. The General...

  4. Design and Practical Evaluation of a Family of Lightweight Protocols for Heterogeneous Sensing through BLE Beacons in IoT Telemetry Applications.

    Science.gov (United States)

    Hernández-Rojas, Dixys L; Fernández-Caramés, Tiago M; Fraga-Lamas, Paula; Escudero, Carlos J

    2017-12-27

    The Internet of Things (IoT) involves a wide variety of heterogeneous technologies and resource-constrained devices that interact with each other. Due to such constraints, IoT devices usually require lightweight protocols that optimize the use of resources and energy consumption. Among the different commercial IoT devices, Bluetooth and Bluetooth Low Energy (BLE)-based beacons, which broadcast periodically certain data packets to notify their presence, have experienced a remarkable growth, specially due to their application in indoor positioning systems. This article proposes a family of protocols named Lightweight Protocol for Sensors (LP4S) that provides fast responses and enables plug-and-play mechanisms that allow IoT telemetry systems to discover new nodes and to describe and auto-register the sensors and actuators connected to a beacon. Thus, three protocols are defined depending on the beacon hardware characteristics: LP4S-6 (for resource-constraint beacons), LP4S-X (for more powerful beacons) and LP4S-J (for beacons able to run complex firmware). In order to demonstrate the capabilities of the designed protocols, the most restrictive (LP4S-6) is tested after implementing it for a telemetry application in a beacon based on Eddystone (Google's open beacon format). Thus, the beacon specification is extended in order to increase its ability to manage unlimited sensors in a telemetry system without interfering in its normal operation with Eddystone frames. The performed experiments show the feasibility of the proposed solution and its superiority, in terms of latency and energy consumption, with respect to approaches based on Generic Attribute Profile (GATT) when multiple users connect to a mote or in scenarios where latency is not a restriction, but where low-energy consumption is essential.

  5. Design and Practical Evaluation of a Family of Lightweight Protocols for Heterogeneous Sensing through BLE Beacons in IoT Telemetry Applications

    Directory of Open Access Journals (Sweden)

    Dixys L. Hernández-Rojas

    2017-12-01

    Full Text Available The Internet of Things (IoT involves a wide variety of heterogeneous technologies and resource-constrained devices that interact with each other. Due to such constraints, IoT devices usually require lightweight protocols that optimize the use of resources and energy consumption. Among the different commercial IoT devices, Bluetooth and Bluetooth Low Energy (BLE-based beacons, which broadcast periodically certain data packets to notify their presence, have experienced a remarkable growth, specially due to their application in indoor positioning systems. This article proposes a family of protocols named Lightweight Protocol for Sensors (LP4S that provides fast responses and enables plug-and-play mechanisms that allow IoT telemetry systems to discover new nodes and to describe and auto-register the sensors and actuators connected to a beacon. Thus, three protocols are defined depending on the beacon hardware characteristics: LP4S-6 (for resource-constraint beacons, LP4S-X (for more powerful beacons and LP4S-J (for beacons able to run complex firmware. In order to demonstrate the capabilities of the designed protocols, the most restrictive (LP4S-6 is tested after implementing it for a telemetry application in a beacon based on Eddystone (Google’s open beacon format. Thus, the beacon specification is extended in order to increase its ability to manage unlimited sensors in a telemetry system without interfering in its normal operation with Eddystone frames. The performed experiments show the feasibility of the proposed solution and its superiority, in terms of latency and energy consumption, with respect to approaches based on Generic Attribute Profile (GATT when multiple users connect to a mote or in scenarios where latency is not a restriction, but where low-energy consumption is essential.

  6. Widespread, Juvenile Alteration of the Ferrar Dolerite in Beacon Valley, Antarctica

    Science.gov (United States)

    Salvatore, M. R.; Mustard, J. F.; Head, J. W.; Cooper, R. F.; Marchant, D. R.

    2011-12-01

    The hyper-arid and hypo-thermal conditions that are pervasive throughout the McMurdo Dry Valleys (MDV) result in slow, immature chemical alteration that is often shadowed by physical erosion and mass wasting processes. Beacon Valley is an exception to this model in that its surface is extremely stable and has been for at least several million years. This stability allows for chemical alteration to progress in the absence of extensive physical erosion. We have collected fourteen Ferrar Dolerite samples from the floor of central Beacon Valley and have analyzed them to assess the range and extent of chemical and mineralogical variations between the unaltered interiors and their altered surfaces. Surface rocks from Beacon Valley, Antarctica, exhibit a distinct red/brown weathering/alteration rind relative to their dark grey interiors as a result of in situ chemical alteration. The rind is readily visible in broken and cut rocks and is typically 0.5-10 mm in thickness. It shows a gradational transition to the unaltered interiors. Reflectance and emission spectroscopy both show distinct disparity between unaltered rock interiors and their corresponding surfaces related to the alteration rind. Interestingly, spectroscopic signatures of primary minerals are clearly present showing that the alteration process does not completely mask the primary rock constituents from remote sensing. Visible-near infrared spectra show distinct ferric absorptions between 0.3 and 1.0 μm indicating a significant increase in ferric iron. Emission spectroscopy shows distinct absorptions related to poorly crystalline alteration products suggesting a breakdown in mineral structure in the optically active zone. EPMA analyses and BSE imagery of the dolerite surfaces and the transition between altered and unaltered regions, however, do not show any obvious chemical or mineralogical variability. This result is supported by flux fusion and ICP-AES analyses that show no substantial chemical variations

  7. Tracking neuronal marker expression inside living differentiating cells using molecular beacons

    DEFF Research Database (Denmark)

    Ilieva, Mirolyuba; Della Vedova, Paolo; Hansen, Ole

    2013-01-01

    Monitoring gene expression is an important tool for elucidating mechanisms of cellular function. In order to monitor gene expression during nerve cell development, molecular beacon (MB) probes targeting markers representing different stages of neuronal differentiation were designed and synthesized...... transfection. The cells will then each contain about 60,000 MBs. Gene expression was detected at different time points using fluorescence microscopy. Nestin and NeuN mRNA were expressed in approximately 35% of the LUHMES cells grown in growth medium, and in 80-90% of cells after differentiation. MAP2...... and tyrosine hydroxylase mRNAs were expressed 2 and 3 days post induction of differentiation, respectively. Oct 4 was not detected with MB in these cells and signal was not increased over time suggesting that MB are generally stable inside the cells. The gene expression changes measured using MBs were...

  8. Model-Based Localization and Tracking Using Bluetooth Low-Energy Beacons

    Directory of Open Access Journals (Sweden)

    F. Serhan Daniş

    2017-10-01

    Full Text Available We introduce a high precision localization and tracking method that makes use of cheap Bluetooth low-energy (BLE beacons only. We track the position of a moving sensor by integrating highly unreliable and noisy BLE observations streaming from multiple locations. A novel aspect of our approach is the development of an observation model, specifically tailored for received signal strength indicator (RSSI fingerprints: a combination based on the optimal transport model of Wasserstein distance. The tracking results of the entire system are compared with alternative baseline estimation methods, such as nearest neighboring fingerprints and an artificial neural network. Our results show that highly accurate estimation from noisy Bluetooth data is practically feasible with an observation model based on Wasserstein distance interpolation combined with the sequential Monte Carlo (SMC method for tracking.

  9. Nanomechanical DNA origami 'single-molecule beacons' directly imaged by atomic force microscopy

    Science.gov (United States)

    Kuzuya, Akinori; Sakai, Yusuke; Yamazaki, Takahiro; Xu, Yan; Komiyama, Makoto

    2011-01-01

    DNA origami involves the folding of long single-stranded DNA into designed structures with the aid of short staple strands; such structures may enable the development of useful nanomechanical DNA devices. Here we develop versatile sensing systems for a variety of chemical and biological targets at molecular resolution. We have designed functional nanomechanical DNA origami devices that can be used as 'single-molecule beacons', and function as pinching devices. Using 'DNA origami pliers' and 'DNA origami forceps', which consist of two levers ~170 nm long connected at a fulcrum, various single-molecule inorganic and organic targets ranging from metal ions to proteins can be visually detected using atomic force microscopy by a shape transition of the origami devices. Any detection mechanism suitable for the target of interest, pinching, zipping or unzipping, can be chosen and used orthogonally with differently shaped origami devices in the same mixture using a single platform. PMID:21863016

  10. Model-Based Localization and Tracking Using Bluetooth Low-Energy Beacons.

    Science.gov (United States)

    Daniş, F Serhan; Cemgil, Ali Taylan

    2017-10-29

    We introduce a high precision localization and tracking method that makes use of cheap Bluetooth low-energy (BLE) beacons only. We track the position of a moving sensor by integrating highly unreliable and noisy BLE observations streaming from multiple locations. A novel aspect of our approach is the development of an observation model, specifically tailored for received signal strength indicator (RSSI) fingerprints: a combination based on the optimal transport model of Wasserstein distance. The tracking results of the entire system are compared with alternative baseline estimation methods, such as nearest neighboring fingerprints and an artificial neural network. Our results show that highly accurate estimation from noisy Bluetooth data is practically feasible with an observation model based on Wasserstein distance interpolation combined with the sequential Monte Carlo (SMC) method for tracking.

  11. MO-FG-BRA-06: Electromagnetic Beacon Insertion in Lung Cancer Patients and Resultant Surrogacy Errors for Dynamic MLC Tumour Tracking

    International Nuclear Information System (INIS)

    Hardcastle, N; Booth, J; Caillet, V; Haddad, C; Crasta, C; O’Brien, R; Keall, P; Szymura, K

    2016-01-01

    Purpose: To assess endo-bronchial electromagnetic beacon insertion and to quantify the geometric accuracy of using beacons as a surrogate for tumour motion in real-time multileaf collimator (MLC) tracking of lung tumours. Methods: The LIGHT SABR trial is a world-first clinical trial in which the MLC leaves move with lung tumours in real time on a standard linear accelerator. Tracking is performed based on implanted electromagnetic beacons (CalypsoTM, Varian Medical Systems, USA) as a surrogate for tumour motion. Five patients have been treated and have each had three beacons implanted endo-bronchially under fluoroscopic guidance. The centre of mass (C.O.M) has been used to adapt the MLC in real-time. The geometric error in using the beacon C.O.M as a surrogate for tumour motion was measured by measuring the tumour and beacon C.O.M in all phases of the respiratory cycle of a 4DCT. The surrogacy error was defined as the difference in beacon and tumour C.O.M relative to the reference phase (maximum exhale). Results: All five patients have had three beacons successfully implanted with no migration between simulation and end of treatment. Beacon placement relative to tumour C.O.M varied from 14 to 74 mm and in one patient spanned two lobes. Surrogacy error was measured in each patient on the simulation 4DCT and ranged from 0 to 3 mm. Surrogacy error as measured on 4DCT was subject to artefacts in mid-ventilation phases. Surrogacy error was a function of breathing phase and was typically larger at maximum inhale. Conclusion: Beacon placement and thus surrogacy error is a major component of geometric uncertainty in MLC tracking of lung tumours. Surrogacy error must be measured on each patient and incorporated into margin calculation. Reduction of surrogacy error is limited by airway anatomy, however should be taken into consideration when performing beacon insertion and planning. This research is funded by Varian Medical Systems via a collaborative research agreement.

  12. Design of a K/Q-Band Beacon Receiver for the Alphasat Technology Demonstration Payload (TDP) #5 Experiment

    Science.gov (United States)

    Morse, Jacquelynne R.

    2014-01-01

    This paper describes the design and performance of a coherent KQ-band (2040 GHz) beacon receiver developed at NASA Glenn Research Center (GRC) that will be installed at the Politecnico di Milano (POLIMI) for use in the Alphasat Technology Demonstration Payload 5 (TDP5) beacon experiment. The goal of this experiment is to characterize rain fade attenuation at 40 GHz to improve the performance of existing statistical rain attenuation models in the Q-band. The ground terminal developed by NASA GRC utilizes an FFT-based frequency estimation receiver capable of characterizing total path attenuation effects due to gaseous absorption, clouds, rain, and scintillation. The receiver system has been characterized in the lab and demonstrates a system dynamic range performance of better than 58 dB at 1 Hz and better than 48 dB at 10 Hz rates.

  13. Aspect Determination Using a Beacon with a Spiral Wave Front: Modeling and Performance Analysis in Operational Environments

    Science.gov (United States)

    2014-12-19

    the NSWC PCD Acoustic Test Facility in FY10 and at the Navy’s Dodge Pond facility operated by the Naval Undersea Warfare Center–Division Newport...minima shown in Fig. 15. The choice of minima will depend on the signal processing requirements of the beacon receiver, either mini - mizing the phase...surface it is also moving over these rods, leading to variation in amplitude. To mini - mize these effects, a sliding average is applied to the data

  14. A case study of pediatric asthma alerts from the beacon community program in cincinnati: technology is just the first step.

    Science.gov (United States)

    Trudnak, Tara; Mansour, Mona; Mandel, Keith; Sauers, Hadley; Pandzik, Gerry; Donisi, Carl; Fairbrother, Gerry

    2014-01-01

    The Beacon Community in Cincinnati, Ohio was an innovative, community-wide initiative to use technology to transform care. One important feature was the development of regional alerts to notify practices when patients were hospitalized or seen in the emergency department. The purpose of this paper is to describe the way in which technology engages the improvement process, and to describe the early stages of learning how to use technology to enhance quality improvement. We interviewed key Beacon leaders as well as providers and office staff in selected practices. We also collected preliminary data from practices that reflected handling of alerts, including the number of asthma related alerts received and followed up. Regional alerts, supplied by the community-wide health information exchange, were a significant addition to the quality improvement effort in that they enabled practices to identify and follow up with additional children at risk. An important finding was the substantial effort at the practice level to integrate technology into ongoing patient care. Developing the technology for community wide alerts represented a significant endeavor in the Cincinnati Beacon Community. However, the technology was just the first step. Despite extra effort and time required on the part of individual practices, they reported that the value of having alerts was high. Hospital and ED visits represent some of the most costly aspects of care, and an efficient process for intervening with children using these costly services was seen as of significant value.

  15. Theranostic Properties of a Survivin-Directed Molecular Beacon in Human Melanoma Cells

    Science.gov (United States)

    Carpi, Sara; Fogli, Stefano; Giannetti, Ambra; Adinolfi, Barbara; Tombelli, Sara; Da Pozzo, Eleonora; Vanni, Alessia; Martinotti, Enrica; Martini, Claudia; Breschi, Maria Cristina; Pellegrino, Mario

    2014-01-01

    Survivin is an inhibitor of apoptosis overexpressed in different types of tumors and undetectable in most terminally differentiated normal tissues. In the current study, we sought to evaluate the in vitro theranostic properties of a molecular beacon-oligodeoxynucleotide (MB) that targets survivin mRNA. We used laser scanning confocal microscopy to study MB delivery in living cells and real-time PCR and western blot to assess selective survivin-targeting in human malignant melanoma cells. We further assess the pro-apoptotic effect of MB by measuring internucleosomal DNA fragmentation, dissipation of mitochondrial membrane potential (MMP) and changes in nuclear morphology. Transfection of MB into A375 and 501 Mel cells generated high signal intensity from the cytoplasm, while no signal was detected in the extracellular environment and in survivin-negative cells (i.e., human melanocytes and monocytes). MB time dependently decreased survivin mRNA and protein expression in melanoma cells with the maximum effect reached at 72 h. Treatment of melanoma cells with MB induced apoptosis by significant changes in MMP, accumulation of histone-complexed DNA fragments in the cytoplasm and nuclear condensation. MB also enhanced the pro-apoptotic effect of standard chemotherapeutic drugs tested at clinically relevant concentrations. The MB tested in the current study conjugates the ability of imaging with the pharmacological silencing activity against survivin mRNA in human melanoma cells and may represent an innovative approach for cancer diagnosis and treatment. PMID:25501971

  16. Quantification of Salmonella Typhi in water and sediments by molecular-beacon based qPCR.

    Science.gov (United States)

    Rani, Neetika; Vajpayee, Poornima; Bhatti, Saurabh; Singh, Smriti; Shanker, Rishi; Gupta, Kailash Chand

    2014-10-01

    A molecular-beacon based qPCR assay targeting staG gene was designed for specific detection and quantification of S. Typhi and validated against water and sediment samples collected from the river Ganga, Yamuna and their confluence on two days during Mahakumbha mela 2012-2013 (a) 18 December, 2012: before six major religious holy dips (Makar Sankranti, Paush Poornima, Mauni Amavasya, Basant Panchami, Maghi Poornima and Mahashivratri) (b) 10 February, 2013: after the holy dip was taken by over 3,00,00,000 devotees led by ascetics of Hindu sects at Sangam on 'Mauni Amavasya' (the most auspicious day of ritualistic mass bathing). The assay could detect linearly lowest 1 genomic equivalent per qPCR and is highly sensitive and selective for S. Typhi detection in presence of non specific DNA from other bacterial strains including S. Paratyphi A and S. Typhimurium. It has been observed that water and sediment samples exhibit S. Typhi. The mass holy dip by devotees significantly affected the water and sediment quality by enhancing the number of S. Typhi in the study area. The qPCR developed in the study might be helpful in planning the intervention and prevention strategies for control of enteric fever outbreaks in endemic regions. Copyright © 2014 Elsevier Inc. All rights reserved.

  17. Learning from health information exchange technical architecture and implementation in seven beacon communities.

    Science.gov (United States)

    McCarthy, Douglas B; Propp, Karen; Cohen, Alexander; Sabharwal, Raj; Schachter, Abigail A; Rein, Alison L

    2014-01-01

    As health care providers adopt and make "meaningful use" of health information technology (health IT), communities and delivery systems must set up the infrastructure to facilitate health information exchange (HIE) between providers and numerous other stakeholders who have a role in supporting health and care. By facilitating better communication and coordination between providers, HIE has the potential to improve clinical decision-making and continuity of care, while reducing unnecessary use of services. When implemented as part of a broader strategy for health care delivery system and payment reform, HIE capability also can enable the use of analytic tools needed for population health management, patient engagement in care, and continuous learning and improvement. The diverse experiences of seven communities that participated in the three-year federal Beacon Community Program offer practical insight into factors influencing the technical architecture of exchange infrastructure and its role in supporting improved care, reduced cost, and a healthier population. The case studies also document challenges faced by the communities, such as significant time and resources required to harmonize variations in the interpretation of data standards. Findings indicate that their progress developing community-based HIE strategies, while driven by local needs and objectives, is also influenced by broader legal, policy, and market conditions.

  18. Space Weather Operation at KASI With Van Allen Probes Beacon Signals

    Science.gov (United States)

    Lee, Jongkil; Kim, Kyung-Chan; Giuseppe, Romeo; Ukhorskiy, Sasha; Sibeck, David; Kessel, Ramona; Mauk, Barry; Giles, Barbara; Gu, Bon-Jun; Lee, Hyesook; Park, Young-Deuk; Lee, Jaejin

    2018-02-01

    The Van Allen Probes (VAPs) are the only modern National Aeronautics and Space Administration (NASA) spacecraft broadcasting real-time data on the Earth's radiation belts for space weather operations. Since 2012, the Korea Astronomy and Space Science Institute (KASI) has contributed to the receipt of these data via a 7 m satellite-tracking antenna and used these beacon data for space weather operations. An approximately 15 min period is required from measurement to acquisition of Level-1 data. In this paper, we demonstrate the use of VAP data for monitoring space weather conditions at geostationary orbit (GEO) by highlighting the Saint Patrick's Day storm of 2015. During that storm, Probe-A observed a significant increase in the relativistic electron flux at 3 RE. Those electrons diffused outward resulting in a large increase of the electron flux >2 MeV at GEO, which potentially threatened satellite operations. Based on this study, we conclude that the combination of VAP data and National Oceanic and Atmospheric Administration-Geostationary Operational Environmental Satellite (NOAA-GOES) data can provide improved space environment information to geostationary satellite operators. In addition, the findings obtained indicate that more data-receiving sites would be necessary and data connections improved if this or a similar system were to be used as an operational data service.

  19. A molecular beacon microarray based on a quantum dot label for detecting single nucleotide polymorphisms.

    Science.gov (United States)

    Guo, Qingsheng; Bai, Zhixiong; Liu, Yuqian; Sun, Qingjiang

    2016-03-15

    In this work, we report the application of streptavidin-coated quantum dot (strAV-QD) in molecular beacon (MB) microarray assays by using the strAV-QD to label the immobilized MB, avoiding target labeling and meanwhile obviating the use of amplification. The MBs are stem-loop structured oligodeoxynucleotides, modified with a thiol and a biotin at two terminals of the stem. With the strAV-QD labeling an "opened" MB rather than a "closed" MB via streptavidin-biotin reaction, a sensitive and specific detection of label-free target DNA sequence is demonstrated by the MB microarray, with a signal-to-background ratio of 8. The immobilized MBs can be perfectly regenerated, allowing the reuse of the microarray. The MB microarray also is able to detect single nucleotide polymorphisms, exhibiting genotype-dependent fluorescence signals. It is demonstrated that the MB microarray can perform as a 4-to-2 encoder, compressing the genotype information into two outputs. Copyright © 2015 Elsevier B.V. All rights reserved.

  20. The keys to governance and stakeholder engagement: the southeast michigan beacon community case study.

    Science.gov (United States)

    Des Jardins, Terrisca R

    2014-01-01

    Community-based health information exchanges (HIEs) and efforts to consolidate and house data are growing, given the advent of Accountable Care Organizations (ACOs) under the Affordable Care Act and other similar population health focused initiatives. The Southeast Michigan Beacon Community (SEMBC) can be looked to as one case study that offers lessons learned, insights on challenges faced and accompanying workarounds related to governance and stakeholder engagement. The SEMBC case study employs an established Data Warehouse Governance Framework to identify and explain the necessary governance and stakeholder engagement components, particularly as they relate to community-wide data sharing and data warehouses or repositories. Perhaps the biggest lesson learned through the SEMBC experience is that community-based work is hard. It requires a great deal of community leadership, collaboration and resources. SEMBC found that organizational structure and guiding principles needed to be continually revisited and nurtured in order to build the relationships and trust needed among stakeholder organizations. SEMBC also found that risks and risk mitigation tactics presented challenges and opportunities at the outset and through the duration of the three year pilot period. Other communities across the country embarking on similar efforts need to consider realistic expectations about community data sharing infrastructures and the accompanying and necessary governance and stakeholder engagement fundamentals.

  1. The use of nanocrystal quantum dot as fluorophore reporters in molecular beacon-based assays

    Science.gov (United States)

    Adegoke, Oluwasesan; Park, Enoch Y.

    2016-12-01

    The utilization of molecular beacon (MB) biosensor probes to detect nucleic acid targets has received enormous interest within the scientific community. This interest has been stimulated by the operational qualities of MB-based probes with respect to their unique sensitivity and specificity. The design of MB biosensors entails not only optimizing the sequence of the loop to hybridize with the nucleic acid target or optimization of the length of the stem to tune the sensitivity but also the selection of the appropriate fluorophore reporter to generate the signal transduction read-out upon hybridization of the probe with the target sequence. Traditional organic fluorescent dyes are mostly used for signal reporting in MB assays but their optical properties in comparison to semiconductor fluorescent quantum dot (Qdot) nanocrystals are at a disadvantage. This review highlights the progress made in exploiting Qdot as fluorophore reporters in MB-based assays with the aim of instigating further development in the field of Qdot-MB technology. The development reported to date indicates that unparalleled fluorescence signal reporting in MB-based assays can be achieved using well-constructed Qdot fluorophores.

  2. The Inversion of Ionospheric/plasmaspheric Electron Density From GPS Beacon Observations

    Science.gov (United States)

    Zou, Y. H.; Xu, J. S.; Ma, S. Y.

    It is a space-time 4-D tomography to reconstruct ionospheric/ plasmaspheric elec- tron density, Ne, from ground-based GPS beacon measurements. The mathematical foundation of such inversion is studied in this paper and some simulation results of reconstruction for GPS network observation are presented. Assuming reasonably a power law dependence of NE on time with an index number of 1-3 during one ob- servational time of GPS (60-90min.), 4-D inversion in consideration is reduced to a 3-D cone-beam tomography with incomplete projections. To see clearly the effects of the incompleteness on the quality of reconstruction for 3-D condition, we deduced theoretically the formulae of 3-D parallel-beam tomography. After establishing the mathematical basis, we adopt linear temporal dependence of NE and voxel elemental functions to perform simulation of NE reconstruction with the help of IRI90 model. Reasonable time-dependent 3-D images of ionosphere/ plasmasphere electron density distributions are obtained when taking proper layout of the GPS network and allowing variable resolutions in vertical.

  3. Electrochemical molecular beacon biosensor for sequence-specific recognition of double-stranded DNA.

    Science.gov (United States)

    Miao, Xiangmin; Guo, Xiaoting; Xiao, Zhiyou; Ling, Liansheng

    2014-09-15

    Direct recognition of double-stranded DNA (dsDNA) was crucial to disease diagnosis and gene therapy, because DNA in its natural state is double stranded. Here, a novel sensor for the sequence-specific recognition of dsDNA was developed based on the structure change of ferrocene (Fc) redox probe modified molecular beacon (MB). For constructing such a sensor, gold nanoparticles (AuNPs) were initially electrochemical-deposited onto glass carbon electrode (GCE) surface to immobilize thiolated MB in their folded states with Au-S bond. Hybridization of MB with target dsDNA induced the formation of parallel triplex DNA and opened the stem-loop structure of it, which resulted in the redox probe (Fc) away from the electrode and triggered the decrease of current signals. Under optimal conditions, dsDNA detection could be realized in the range from 350 pM to 25 nM, with a detection limit of 275 pM. Moreover, the proposed method has good sequence-specificity for target dsDNA compared with single base pair mismatch and two base pairs mismatches. Copyright © 2014 Elsevier B.V. All rights reserved.

  4. Atmospheric Beacons of Life from Exoplanets Around G and K Stars

    Science.gov (United States)

    Airapetian, Vladimir S.; Jackman, Charles H.; Mlynczak, Martin; Danchi, William; Hunt, Linda

    2017-01-01

    The current explosion in detection and characterization of thousands of extrasolar planets from the Kepler mission, the Hubble Space Telescope, and large ground-based telescopes opens a new era in searches for Earth-analog exoplanets with conditions suitable for sustaining life. As more Earth-sized exoplanets are detected in the near future, we will soon have an opportunity to identify habitale worlds. Which atmospheric biosignature gases from habitable planets can be detected with our current capabilities? The detection of the common biosignatures from nitrogen-oxygen rich terrestrial-type exoplanets including molecular oxygen (O2), ozone (O3), water vapor (H2O), carbon dioxide (CO2), nitrous oxide (N2O), and methane (CH4) requires days of integration time with largest space telescopes, and thus are very challenging for current instruments. In this paper we propose to use the powerful emission from rotational-vibrational bands of nitric oxide, hydroxyl and molecular oxygen as signatures of nitrogen, oxygen, and water rich atmospheres of terrestrial type exoplanets "highlighted" by the magnetic activity from young G and K main-sequence stars. The signals from these fundamental chemical prerequisites of life we call atmospheric "beacons of life" create a unique opportunity to perform direct imaging observations of Earth-sized exoplanets with high signal-to-noise and low spectral resolution with the upcoming NASA missions.

  5. Outage Probability Analysis in Power-Beacon Assisted Energy Harvesting Cognitive Relay Wireless Networks

    Directory of Open Access Journals (Sweden)

    Ngoc Phuc Le

    2017-01-01

    Full Text Available We study the performance of the secondary relay system in a power-beacon (PB assisted energy harvesting cognitive relay wireless network. In our system model, a secondary source node and a relay node first harvest energy from distributed PBs. Then, the source node transmits its data to the destination node with the help of the relay node. Also, fading coefficients of the links from the PBs to the source node and relay node are assumed independent but not necessarily identically distributed (i.n.i.d Nakagami-m random variables. We derive exact expressions for the power outage probability and the channel outage probability. Based on that, we analyze the total outage probability of the secondary relay system. Asymptotic analysis is also performed, which provides insights into the system behavior. Moreover, we evaluate impacts of the primary network on the performance of the secondary network with respect to the tolerant interference threshold at the primary receiver as well as the interference introduced by the primary transmitter at the secondary source and relay nodes. Simulation results are provided to validate the analysis.

  6. Chitosan Combined with Molecular Beacon for Mir-155 Detection and Imaging in Lung Cancer

    Directory of Open Access Journals (Sweden)

    Hai-Zhen Zhu

    2014-09-01

    Full Text Available Lung cancer is the major cause of cancer-related deaths worldwide, thus developing effective methods for its early diagnosis is urgently needed. In recent years, microRNAs (miRNAs, miR have been reported to play important roles in carcinogenesis and have become potential biomarkers for cancer diagnosis and treatment. Molecular beacon (MB technology is a universal technology to detect DNA/RNA expression in living cells. As a natural polymers, chitosan (CS nanoparticles could be used as a carrier for safe delivery of nucleic acid. In this study, we developed a probe using nanoparticles of miR-155 MB self assembled with CS (CS-miR-155 MB to image the expression of miR-155 in cancer cells. Hybridization assay showed that the locked nucleic acid (LAN modified miR-155 MB could target miR-155 effectively and sensitively. The miR-155 MB self-assembly with CS nanoparticles formed stable complexes at the proper weight ratio. The CS nanoparticles showed higher fluorescence intensity and transfection efficiency than the lipid-based formulation transfection agent by confocal microscopy and flow cytometry analysis. The CS-MB complexes were found to be easily synthesized and exhibited strong enzymatic stability, efficient cellular uptake, high target selectivity and biocompatibility. The CS-MB complexes can also be applied in other cancers just by simply changing for a targeted miRNA highly expressed in those cancer cells. Therefore, it is a promising vehicle used for detecting miRNA expression in living cells.

  7. Hybridization Efficiency of Molecular Beacons Bound to Gold Nanowires: Effect of Surface Coverage and Target Length

    Science.gov (United States)

    2010-01-01

    Surface-bound nucleic acid probes designed to adopt specific secondary structures are becoming increasingly important in a range of biosensing applications but remain less well characterized than traditional single-stranded probes, which are typically designed to avoid secondary structure. We report the hybridization efficiency for surface-immobilized hairpin DNA probes. Our probes are molecular beacons, carrying a 3′ dye moiety and a 5′ thiol for attachment to gold nanowires, which serve as both scaffolds for probe attachment and quenchers. Hybridization efficiency was dependent on probe surface coverage, reaching a maximum of ∼90% at intermediate coverages of (1−2) × 1012 probes/cm2 and dropping to ≤20% at higher or lower coverages. Fluorescence intensity did not track with the number of target molecules bound, and was highest for high probe coverage despite the lower bound targets per square centimeter. Backfilling with short thiolated oligoethylene glycol spacers increased hybridization efficiency at low hairpin probe coverages (∼(3−4) × 1011 probes/cm2), but not at higher probe coverages (1 × 1012/cm2). We also evaluated the effect of target length by adding up to 50 nonhybridizing nucleotides to the 3′ or 5′ end of the complementary target sequence. Additional nucleotides on the 3′ end of the complementary target sequence (i.e., the end near the nanowire surface) had a much greater impact on hybridization efficiency as compared to nucleotides added to the 5′ end. This work provides guidance in designing sensors in which surface-bound probes designed to adopt secondary structures are used to detect target sequences from solution. PMID:21038880

  8. Experimental Analysis of Sublimation Dynamics for Buried Glacier Ice in Beacon Valley, Antarctica

    Science.gov (United States)

    Ehrenfeucht, S.; Dennis, D. P.; Marchant, D. R.

    2017-12-01

    The age of the oldest known buried ice in Beacon Valley, McMurdo Dry Valleys (MDV) Antarctica is a topic of active debate due to its implications for the stability of the East Antarctic Ice Sheet. Published age estimates range from as young as 300 ka to as old as 8.1 Ma. In the upland MDV, ablation occurs predominantly via sublimation. The relict ice in question (ancient ice from Taylor Glacier) lies buried beneath a thin ( 30-70 cm) layer of sublimation till, which forms as a lag deposit as underlying debris-rich ice sublimes. As the ice sublimates, the debris held within the ice accumulates slowly on the surface, creating a porous boundary between the buried-ice surface and the atmosphere, which in turn influences gas exchange between the ice and the atmosphere. Additionally, englacial debris adds several salt species that are ultimately concentrated on the ice surface. It is well documented the rate of ice sublimation varies as a function of overlying till thickness. However, the rate-limiting dynamics under varying environmental conditions, including the threshold thicknesses at which sublimation is strongly retarded, are not yet defined. To better understand the relationships between sublimation rate, till thickness, and long-term surface evolution, we build on previous studies by Lamp and Marchant (2017) and evaluate the role of till thickness as a control on ice loss in an environmental chamber capable of replicating the extreme cold desert conditions observed in the MDV. Previous work has shown that this relationship exhibits exponential decay behavior, with sublimation rate significantly dampened under less than 10 cm of till. In our experiments we pay particular attention to the effect of the first several cm of till in order to quantify the dynamics that govern the transition from bare ice to debris-covered ice. We also examine this transition for various forms of glacier ice, including ice with various salt species.

  9. A Biofunctional Molecular Beacon for Detecting Single Base Mutations in Cancer Cells

    Directory of Open Access Journals (Sweden)

    Haiyan Dong

    2016-01-01

    Full Text Available The development of a convenient and sensitive biosensing system to detect specific DNA sequences is an important issue in the field of genetic disease therapy. As a classic DNA detection technique, molecular beacon (MB is often used in the biosensing system. However, it has intrinsic drawbacks, including high assay cost, complicated chemical modification, and operational complexity. In this study, we developed a simple and cost-effective label-free multifunctional MB (LMMB by integrating elements of polymerization primer, template, target recognition, and G-quadruplex into one entity to detect target DNA. The core technique was accomplished by introducing a G-hairpin that features fragments of both G-quadruplex and target DNA recognition in the G-hairpin stem. Hybridization between LMMB and target DNA triggered conformational change between the G-hairpin and the common C-hairpin, resulting in significant SYBR-green signal amplification. The hybridization continues to the isothermal circular strand-displacement polymerization and accumulation of the double-stranded fragments, causing the uninterrupted extension of the LMMB without a need of chemical modification and other assistant DNA sequences. The novel and programmable LMMB could detect target DNA with sensitivity at 250 pmol/l with a linear range from 2 to 100 nmol/l and the relative standard deviation of 7.98%. The LMMB could sense a single base mutation from the normal DNA, and polymerase chain reaction (PCR amplicons of the mutant-type cell line from the wild-type one. The total time required for preparation and assaying was only 25 minutes. Apparently, the LMMB shows great potential for detecting DNA and its mutations in biosamples, and therefore it opens up a new prospect for genetic disease therapy.

  10. Shape and Size Characteristics of Bedload Transported During Winter Storm Events in the Cwm Treweryn Stream, Brecon Beacons, South Wales

    OpenAIRE

    WALSH, TUNCER DEMİR & RORY PETER DOMINIC

    2005-01-01

    Bedload transport in the Cwm Treweryn stream, a tributary of the Usk in the Brecon Beacons, South Wales, was investigated during a wet winter period in early 1995 using basket-type bedload traps and the tracing of painted clasts. The Cwm Treweryn is a typical mountain stream of the region with its gravel-bedload, flashy regime, high gradient and pool-riffle sequences. In particular, the study explored - for a range of categories of clast size - the nature of any influence of clast shape on be...

  11. Potential effects of the introduction of the discrete address beacon system data link on air/ground information transfer problems

    Science.gov (United States)

    Grayson, R. L.

    1981-01-01

    This study of Aviation Safety Reporting System reports suggests that benefits should accure from implementation of discrete address beacon system data link. The phase enhanced terminal information system service is expected to provide better terminal information than present systems by improving currency and accuracy. In the exchange of air traffic control messages, discrete address insures that only the intended recipient receives and acts on a specific message. Visual displays and printer copy of messages should mitigate many of the reported problems associated with voice communications. The problems that remain unaffected include error in addressing the intended recipient and messages whose content is wrong but are otherwise correct as to format and reasonableness.

  12. A tumor mRNA-mediated bi-photosensitizer molecular beacon as an efficient imaging and photosensitizing agent.

    Science.gov (United States)

    Gao, Yuan; Qiao, Guangming; Zhuo, Linhai; Li, Na; Liu, Ying; Tang, Bo

    2011-05-14

    A bi-photosensitizer molecular beacon (bi-PS MB) is assembled by coupling two PS molecules, respectively, onto the opposite ends of a single MB. The MB can be triggered by a tumor marker-survivin mRNA. Fluorescence and cytotoxic (1)O(2) generation occur effectively in breast cancer cells, but not in normal cells. Compared with a single-PS MB, a bi-PS MB exhibits much-enhanced properties in the signal-to-background ratio and (1)O(2) generation simultaneously. © The Royal Society of Chemistry 2011

  13. A catalytic and dual recycling amplification ATP sensor based on target-driven allosteric structure switching of aptamer beacons.

    Science.gov (United States)

    Peng, Ying; Li, Daxiu; Yuan, Ruo; Xiang, Yun

    2018-05-15

    Abnormal concentrations of ATP are associated with many diseases and cancers, and quantitative detection of ATP is thus of great importance for disease diagnosis and prognosis. In the present work, we report a new dual recycling amplification sensor integrated with catalytic hairpin assembly (CHA) to achieve high sensitivity for fluorescent detection of ATP. The association of the target ATP with the aptamer beacons causes the allosteric structure switching of the aptamer beacons to expose the toehold regions, which hybridize with and unfold the fluorescently quenched hairpin signal probes (HP1) to recycle the target ATP and to trigger CHA between HP1 and the secondary hairpin probes (HP2) to form HP1/HP2 duplexes. Due to the recycling amplification, the presence of ATP leads to the formation of many HP1/HP2 duplexes, generating dramatically amplified fluorescent signals for sensitive detection of ATP. Under optimal experimental conditions, our sensor linearly responds to ATP in the range from 25 to 600nM with a calculated detection limit of 8.2nM. Furthermore, the sensor shows a high selectivity and can also be used to detect ATP in human serums to realize its application for real samples. With the distinct advantage of significant signal amplification without the involvement of any nanomaterial and enzyme, the developed sensor thus holds great potential for simple and sensitive detection of different small molecules and proteins. Copyright © 2018 Elsevier B.V. All rights reserved.

  14. Design and Analysis of A Beacon-Less Routing Protocol for Large Volume Content Dissemination in Vehicular Ad Hoc Networks.

    Science.gov (United States)

    Hu, Miao; Zhong, Zhangdui; Ni, Minming; Baiocchi, Andrea

    2016-11-01

    Large volume content dissemination is pursued by the growing number of high quality applications for Vehicular Ad hoc NETworks(VANETs), e.g., the live road surveillance service and the video-based overtaking assistant service. For the highly dynamical vehicular network topology, beacon-less routing protocols have been proven to be efficient in achieving a balance between the system performance and the control overhead. However, to the authors' best knowledge, the routing design for large volume content has not been well considered in the previous work, which will introduce new challenges, e.g., the enhanced connectivity requirement for a radio link. In this paper, a link Lifetime-aware Beacon-less Routing Protocol (LBRP) is designed for large volume content delivery in VANETs. Each vehicle makes the forwarding decision based on the message header information and its current state, including the speed and position information. A semi-Markov process analytical model is proposed to evaluate the expected delay in constructing one routing path for LBRP. Simulations show that the proposed LBRP scheme outperforms the traditional dissemination protocols in providing a low end-to-end delay. The analytical model is shown to exhibit a good match on the delay estimation with Monte Carlo simulations, as well.

  15. BEACON/MOD2A: a computer program for subcompartment analysis of nuclear reactor containment. A user's manual. [PWR

    Energy Technology Data Exchange (ETDEWEB)

    Wells, R. A.

    1979-03-01

    The BEACON code is a Best Estimate Advanced Containment code which being developed by EG and G, Idaho, Inc., at the Idaho National Engineering Laboratory. The program is designed to perform a best estimate analysis of the flow of a mixture of air, water, and steam in a nuclear reactor containment system under loss-of-coolant accident conditions. The code can simulate two-component, two-phase fluid flow in complex geometries using a combination of two-dimensional, one-dimensional, and lumped-parameter representations for the various parts of the system. The current version of BEACON, which is designated BEACON/MOD2A, contains mass and heat transfer models for wall film and for wall conduction. It is suitable for the evaluation of short term transients in PWR dry containment systems. This manual describes the models employed in BEACON/MOD2A and specifies code implementation requirements. It provides application information for input data preparation and for output data interpretation.

  16. A multiplex protein-free lateral flow assay for detection of microRNAs based on unmodified molecular beacons.

    Science.gov (United States)

    Javani, Atefeh; Javadi-Zarnaghi, Fatemeh; Rasaee, Mohammad Javad

    2017-11-15

    Lateral flow assays (LFAs) have promising potentials for point-of-care applications. Recently, many LFAs have been reported that are based on hybridization of oligonucleotide strands. Mostly, biotinylated capture DNAs are immobilized on the surface of a nitrocellulose membrane via streptavidin interactions. During the assay, stable colorful complexes get formed that are visible by naked eyes. Here, we present an inexpensive and unique design of LFA that applies unmodified oligonucleotides at capture lines. The presented LFA do not utilize streptavidin or any other affinity protein. We employ structural switch of molecular beacons (MB) in combination with base stacking hybridization (BSH) phenomenon. The unique design of the reported LFA provided high selectivity for target oligonucleotides. We validated potential applications of the system for detection of DNA mimics of two microRNAs in multiplex assays. Copyright © 2017 Elsevier Inc. All rights reserved.

  17. Gastroesophageal reflux in relation to adenocarcinomas of the esophagus: a pooled analysis from the Barrett's and Esophageal Adenocarcinoma Consortium (BEACON.

    Directory of Open Access Journals (Sweden)

    Michael B Cook

    Full Text Available Previous studies have evidenced an association between gastroesophageal reflux and esophageal adenocarcinoma (EA. It is unknown to what extent these associations vary by population, age, sex, body mass index, and cigarette smoking, or whether duration and frequency of symptoms interact in predicting risk. The Barrett's and Esophageal Adenocarcinoma Consortium (BEACON allowed an in-depth assessment of these issues.Detailed information on heartburn and regurgitation symptoms and covariates were available from five BEACON case-control studies of EA and esophagogastric junction adenocarcinoma (EGJA. We conducted single-study multivariable logistic regressions followed by random-effects meta-analysis. Stratified analyses, meta-regressions, and sensitivity analyses were also conducted.Five studies provided 1,128 EA cases, 1,229 EGJA cases, and 4,057 controls for analysis. All summary estimates indicated positive, significant associations between heartburn/regurgitation symptoms and EA. Increasing heartburn duration was associated with increasing EA risk; odds ratios were 2.80, 3.85, and 6.24 for symptom durations of <10 years, 10 to <20 years, and ≥20 years. Associations with EGJA were slighter weaker, but still statistically significant for those with the highest exposure. Both frequency and duration of heartburn/regurgitation symptoms were independently associated with higher risk. We observed similar strengths of associations when stratified by age, sex, cigarette smoking, and body mass index.This analysis indicates that the association between heartburn/regurgitation symptoms and EA is strong, increases with increased duration and/or frequency, and is consistent across major risk factors. Weaker associations for EGJA suggest that this cancer site has a dissimilar pathogenesis or represents a mixed population of patients.

  18. How a Beacon Community Program in New Orleans Helped Create a Better Health Care System by Building Relationships before Technology.

    Science.gov (United States)

    Khurshid, Anjum; Brown, Lisanne

    2014-01-01

    In the aftermath of Hurricane Katrina, much of New Orleans' healthcare infrastructure was destroyed. Initial federal funding after the storm expanded primary care services and helped set up medical homes for New Orleans' large uninsured and underinsured population. Following that, the Beacon Community in New Orleans, charged with improving health care through the use of technology, decided the best way to accomplish those goals was to build community partnerships and introduce technology improvements based on their input and on their terms. The purpose of this paper is to describe how those partnerships were wrought, including the innovative use of a conceptual framework, and how they are being sustained; how different technologies were and are being introduced; and what the results have been so far. Past successful community experiences, as well as a proven conceptual framework, were used to help establish community partnerships and governance structures, as well as to demonstrate their linkages. This paper represents a compilation of reports and information from key Beacon leaders, staff and providers and their firsthand experiences in setting up those structures, as well as their conclusions. The community partnerships proved extremely successful in not only devising successful ways to introduce new technology into healthcare settings, but in sustaining those changes by creating a governance structure that has enough fluidity to adapt to changing circumstances. Building and developing community partnerships takes time and effort; however, these relationships are necessary and essential to introducing and sustaining new technologies in a healthcare setting and should be a first step for any organization looking to accomplish such goals.

  19. In vitro RNA release from a human rhinovirus monitored by means of a molecular beacon and chip electrophoresis.

    Science.gov (United States)

    Weiss, Victor U; Bliem, Christina; Gösler, Irene; Fedosyuk, Sofiya; Kratzmeier, Martin; Blaas, Dieter; Allmaier, Günter

    2016-06-01

    Liquid-phase electrophoresis either in the classical capillary format or miniaturized (chip CE) is a valuable tool for quality control of virus preparations and for targeting questions related to conformational changes of viruses during infection. We present an in vitro assay to follow the release of the RNA genome from a human rhinovirus (common cold virus) by using a molecular beacon (MB) and chip CE. The MB, a probe that becomes fluorescent upon hybridization to a complementary sequence, was designed to bind close to the 3' end of the viral genome. Addition of Trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid), a well-known additive for reduction of bleaching and blinking of fluorophores in fluorescence microscopy, to the background electrolyte increased the sensitivity of our chip CE set-up. Hence, a fast, sensitive and straightforward method for the detection of viral RNA is introduced. Additionally, challenges of our assay will be discussed. In particular, we found that (i) desalting of virus preparations prior to analysis increased the recorded signal and (ii) the MB-RNA complex signal decreased with the time of virus storage at -70 °C. This suggests that 3'-proximal sequences of the viral RNA, if not the whole genome, underwent degradation during storage and/or freezing and thawing. In summary, we demonstrate, for two independent virus batches, that chip electrophoresis can be used to monitor MB hybridization to RNA released upon incubation of the native virus at 56 °C. Graphical Abstract Schematic of the study strategy: RNA released from HRV-A2 is detected by chip electrophoresis through the increase in fluorescence after genom complexation to a cognate molecular beacon.

  20. Efficacy and safety of QVA149 compared to the concurrent administration of its monocomponents indacaterol and glycopyrronium: the BEACON study

    Directory of Open Access Journals (Sweden)

    Dahl R

    2013-10-01

    Full Text Available Ronald Dahl,1 Dalal Jadayel,2 Vijay KT Alagappan,3 Hungta Chen,3 Donald Banerji31Department of Dermatology, Allergy Centre, Odense University Hospital, Odense, Denmark; 2Novartis Horsham Research Centre, Horsham, UK; 3Novartis Pharmaceuticals Corporation, East Hanover, NJ, USAIntroduction: The BEACON study evaluated the efficacy and safety of QVA149, a once-daily dual bronchodilator containing a fixed-dose combination of the long-acting β2-agonist (LABA indacaterol and long-acting muscarinic antagonist (LAMA glycopyrronium (NVA237, in development for the treatment of patients with chronic obstructive pulmonary disease (COPD, compared with the free-dose concurrent administration of indacaterol plus glycopyrronium (IND+GLY.Methods: In this multicenter, double-blind, parallel group study, patients with stage II or stage III COPD (Global initiative for chronic Obstructive Lung Disease [GOLD] 2010 were randomized (1:1 to once-daily QVA149 (110 µg indacaterol/50 µg glycopyrronium or concurrent administration of indacaterol (150 µg and glycopyrronium (50 µg via the Breezhaler® device (Novartis AG, Basel, Switzerland for 4 weeks. The primary endpoint was to evaluate the noninferiority of QVA149 as compared with concurrent administration of IND+GLY, for trough forced expiratory volume in 1 second (FEV1 after 4 weeks of treatment. The other assessments included FEV1 area under the curve from 0 to 4 hours (AUC0–4 hours at day 1 and week 4, symptom scores, rescue medication use, safety, and tolerability over the 4-week study period.Results: Of 193 patients randomized, 187 (96.9% completed the study. Trough FEV1 at week 4 for QVA149 and IND+GLY was 1.46 L ± 0.02 and 1.46 L ± 0.18, respectively. The FEV1 AUC0–4 hours at day 1 and week 4 were similar between the two treatment groups. Both treatment groups had a similar reduction in symptom scores and rescue medication use for the 4-week treatment period. Overall, 25.6% of patients in QVA149 group

  1. Comparison of mapped and measured total ionospheric electron content using global positioning system and beacon satellite observations

    International Nuclear Information System (INIS)

    Lanyi, G.E.; Roth, T.

    1988-01-01

    Total ionospheric electron contents (TEC) were measured by global positioning system (GPS) dual-frequency receivers developed by the Jet Propulsion Laboratory. The measurements included P-code (precise ranging code) and carrier phase data for six GPS satellites during multiple five-hour observing sessions. A set of these GPS TEC measurements were mapped from the GPS lines of sight to the line of sight of a Faraday beacon satellite by statistically fitting the TEC data to a simple model of the ionosphere. The mapped GPS TEC values were compared with the Faraday rotation measurements. Because GPS transmitter offsets are different for each satellite and because some GPS receiver offsets were uncalibrated, the sums of the satellite and receiver offsets were estimated simultaneously with the TEC in a least squares procedure. The accuracy of this estimation procedure is evaluated indicating that the error of the GPS-determined line of sight TEC can be at or below 1 x 10 to the 16th el/sq cm. Consequently, the current level of accuracy is comparable to the Faraday rotation technique; however, GPS provides superior sky coverage. 15 references

  2. Determination of mutated genes in the presence of wild-type DNA by using molecular beacons as probe

    Science.gov (United States)

    Zhang, Yonghua; Ai, Junjie; Gu, Qiaorong; Gao, Qiang; Qi, Honglan; Zhang, Chengxiao

    2017-03-01

    Low-abundance mutations in the presence of wild-type DNA can be determined using molecular beacon (MB) as probe. A MB is generally used as DNA probe because it can distinguish single-base mismatched target DNA from fully matched target DNA. However, the probe can not determine low-abundance mutations in the presence of wild-type DNA. In this study, this limitation is addressed by enhancing the stability of unpaired base-containing dsDNA with a hydrogen-bonding ligand, which was added after hybridization of the MB to the target DNA. The ligand formed hydrogen bonds with unpaired bases and stabilized the unpaired base-containing dsDNA if target DNA is mutated one. As a result, more MBs were opened by the mutant genes in the presence of the ligand and a further increase in the fluorescence intensity was obtained. By contrast, fluorescence intensity did not change if target DNA is wild-type one. Consequent increase in the fluorescence intensity of the MB was regarded as a signal derived from mutant genes. The proposed method was applied in synthetic template systems to determine point mutation in DNA obtained from PCR analysis. The method also allows rapid and simple discrimination of a signal if it is originated in the presence of mutant gene or alternatively by a lower concentration of wild gene.

  3. Location-Enhanced Activity Recognition in Indoor Environments Using Off the Shelf Smart Watch Technology and BLE Beacons.

    Science.gov (United States)

    Filippoupolitis, Avgoustinos; Oliff, William; Takand, Babak; Loukas, George

    2017-05-27

    Activity recognition in indoor spaces benefits context awareness and improves the efficiency of applications related to personalised health monitoring, building energy management, security and safety. The majority of activity recognition frameworks, however, employ a network of specialised building sensors or a network of body-worn sensors. As this approach suffers with respect to practicality, we propose the use of commercial off-the-shelf devices. In this work, we design and evaluate an activity recognition system composed of a smart watch, which is enhanced with location information coming from Bluetooth Low Energy (BLE) beacons. We evaluate the performance of this approach for a variety of activities performed in an indoor laboratory environment, using four supervised machine learning algorithms. Our experimental results indicate that our location-enhanced activity recognition system is able to reach a classification accuracy ranging from 92% to 100%, while without location information classification accuracy it can drop to as low as 50% in some cases, depending on the window size chosen for data segmentation.

  4. Location-Enhanced Activity Recognition in Indoor Environments Using Off the Shelf Smart Watch Technology and BLE Beacons

    Directory of Open Access Journals (Sweden)

    Avgoustinos Filippoupolitis

    2017-05-01

    Full Text Available Activity recognition in indoor spaces benefits context awareness and improves the efficiency of applications related to personalised health monitoring, building energy management, security and safety. The majority of activity recognition frameworks, however, employ a network of specialised building sensors or a network of body-worn sensors. As this approach suffers with respect to practicality, we propose the use of commercial off-the-shelf devices. In this work, we design and evaluate an activity recognition system composed of a smart watch, which is enhanced with location information coming from Bluetooth Low Energy (BLE beacons. We evaluate the performance of this approach for a variety of activities performed in an indoor laboratory environment, using four supervised machine learning algorithms. Our experimental results indicate that our location-enhanced activity recognition system is able to reach a classification accuracy ranging from 92% to 100%, while without location information classification accuracy it can drop to as low as 50% in some cases, depending on the window size chosen for data segmentation.

  5. Man Overboard Beacon

    National Research Council Canada - National Science Library

    Frank, Thomas A

    2005-01-01

    .... A battery powers the multiple indicators contained in the housing, which include a high intensity strobe light to provide a visual indication, an x-band radar patch antenna to transmit a radar signal...

  6. Beacons of Sound

    DEFF Research Database (Denmark)

    Knakkergaard, Martin

    2018-01-01

    The chapter discusses expectations and imaginations vis-à-vis the concert hall of the twenty-first century. It outlines some of the central historical implications of western culture’s haven for sounding music. Based on the author’s study of the Icelandic concert-house Harpa, the chapter considers...... how these implications, together with the prime mover’s visions, have been transformed as private investors and politicians took over. The chapter furthermore investigates the objectives regarding musical sound and the far-reaching demands concerning acoustics that modern concert halls are required...

  7. Opportunistic Beacon Networks

    NARCIS (Netherlands)

    Türkes, Okan

    2016-01-01

    Modern society is surrounded by an ample spectrum of personal mobile devices with short-range wireless communication support. This ubiquity creates an immense potential of new concepts for people-centric ad hoc networks that can be applied to every personal and social dimension of life. The last

  8. SOX2 and OCT4 mRNA-Expressing Cells, Detected by Molecular Beacons, Localize to the Center of Neurospheres during Differentiation

    DEFF Research Database (Denmark)

    Ilieva, Mirolyuba; Dufva, Martin

    2013-01-01

    image analysis every day for seven days showed that the OCT4 and SOX2 mRNA-expressing cells clustered in the centre of the neurospheres cultured in differentiation medium. By contrast, cells at the periphery of the differentiating spheres developed neurite outgrowths and expressed the tyrosine...... hydroxylase protein, indicating terminal differentiation. Neurospheres cultured in growth medium contained OCT4 and SOX2-positive cells distributed throughout the entire sphere, and no differentiating neurones. Gene expression of SOX2 and OCT4 mRNA detected by MBs correlated well with gene and protein......Neurospheres are used as in vitro assay to measure the properties of neural stem cells. To investigate the molecular and phenotypic heterogeneity of neurospheres, molecular beacons (MBs) targeted against the stem cell markers OCT4 and SOX2 were designed, and synthesized with a 2'-O-methyl RNA...

  9. Analysis of the condition of the SNAP 7-E acoustic beacon installation after 64 months at 2,650 fathoms. Final report Nov 1970--May 1971

    Energy Technology Data Exchange (ETDEWEB)

    Jenkins, J.F.

    1972-02-01

    An isotopic generator powered acoustic beacon installation was recovered after 64 months of exposure to the seawater and bottom sediments at 2,650 fathoms of depth in the Atlantic Ocean south of Bermuda. An analysis of the deterioration of the components of the installation showed that the basic steel support structure and the isotopic generator housing suffered only minimal attack. The lifetimes of these elements are projected to exceed ten years at this site. Stainless steel components of the installation were severely corroded and the use of stainless steel is not recommended. The bottom environment in this location was found to be a typical and similar installations at other sites would have significantly shorter lifetimes.

  10. A Novel Algorithm for Determining the Contextual Characteristics of Movement Behaviors by Combining Accelerometer Features and Wireless Beacons: Development and Implementation.

    Science.gov (United States)

    Magistro, Daniele; Sessa, Salvatore; Kingsnorth, Andrew P; Loveday, Adam; Simeone, Alessandro; Zecca, Massimiliano; Esliger, Dale W

    2018-04-20

    Unfortunately, global efforts to promote "how much" physical activity people should be undertaking have been largely unsuccessful. Given the difficulty of achieving a sustained lifestyle behavior change, many scientists are reexamining their approaches. One such approach is to focus on understanding the context of the lifestyle behavior (ie, where, when, and with whom) with a view to identifying promising intervention targets. The aim of this study was to develop and implement an innovative algorithm to determine "where" physical activity occurs using proximity sensors coupled with a widely used physical activity monitor. A total of 19 Bluetooth beacons were placed in fixed locations within a multilevel, mixed-use building. In addition, 4 receiver-mode sensors were fitted to the wrists of a roving technician who moved throughout the building. The experiment was divided into 4 trials with different walking speeds and dwelling times. The data were analyzed using an original and innovative algorithm based on graph generation and Bayesian filters. Linear regression models revealed significant correlations between beacon-derived location and ground-truth tracking time, with intraclass correlations suggesting a high goodness of fit (R 2 =.9780). The algorithm reliably predicted indoor location, and the robustness of the algorithm improved with a longer dwelling time (>100 s; error location of an individual within an indoor environment. This novel implementation of "context sensing" will facilitate a wealth of new research questions on promoting healthy behavior change, the optimization of patient care, and efficient health care planning (eg, patient-clinician flow, patient-clinician interaction). ©Daniele Magistro, Salvatore Sessa, Andrew P Kingsnorth, Adam Loveday, Alessandro Simeone, Massimiliano Zecca, Dale W Esliger. Originally published in JMIR Mhealth and Uhealth (http://mhealth.jmir.org), 20.04.2018.

  11. Rapid genetic typing of diarrheagenic Escherichia coli using a two-tube modified molecular beacon based multiplex real-time PCR assay and its clinical application.

    Science.gov (United States)

    Chen, Qingliang; Shi, Xiaolu; Li, Yinghui; Jiang, Yixiang; Lin, Yiman; Qiu, Yaqun; Li, Qingge; Hu, Qinghua

    2014-07-15

    Diarrheagenic Escherichia coli (DEC), including Enterotoxigenic E.coli (ETEC), Enteroaggregative E.coli (EAEC), Enteropathogenic E.coli (EPEC), Enterohemolysin E.coli (EHEC) and Enteroinvasive E.coli (EIEC) causes diarrhea or hemolytic uremic syndromes among infants and travelers around the world. A rapid, reliable and repeatable method is urgent for identifying DEC so as to provide the reference for responding to diarrheal disease outbreak and the treatment of the diarrheal patients associated with DEC. In this study, specific primers and modified molecular beacon probes of nine specific virulence genes, whose 5'end were added with homo tail sequence, were designed; and a two-tube modified molecular beacon based multiplex real-time PCR (rtPCR) assay for the identification of five Escherichia coli pathotypes, including ETEC, EAEC, EPEC, EHEC and EIEC was developed and optimized. Totally 102 bacterial strains, including 52 reference bacterial strains and 50 clinical strains were detected to confirm whether the target genes selected were specific. Then detection limits of the assay were tested. Lastly, the assay was applied to the detection of 11860 clinical samples to evaluate the specificity and sensitivity of the developed assay compared with the conventional PCR. The target genes were 100% specific as assessed on 102 bacterial strains since no cross-reactions were observed. The detection limits ranged from 88 CFU/mL (EHEC) to 880 CFU/mL (EPEC). Compared with the conventional PCR, the specificity and sensitivity of the multiplex rtPCR was 100% and over 99%, respectively. The coefficient of variation (CV) for each target gene ranged from 0.45% to 1.53%. 171 positive clinical samples were mostly identified as ETEC (n = 111, 64.9%) and EPEC (n = 38, 22.2%), which were the dominating pathotypes of DEC strains. The developed multiplex rtPCR assay for the identification of DEC was high sensitive and specific and could be applied to the rapid identification of

  12. The G-BHQ synergistic effect: Improved double quenching molecular beacons based on guanine and Black Hole Quencher for sensitive simultaneous detection of two DNAs.

    Science.gov (United States)

    Xiang, Dongshan; Li, Fengquan; Wu, Chenyi; Shi, Boan; Zhai, Kun

    2017-11-01

    We designed two double quenching molecular beacons (MBs) with simple structure based on guanine (G base) and Black Hole Quencher (BHQ), and developed a new analytical method for sensitive simultaneous detection of two DNAs by synchronous fluorescence analysis. In this analytical method, carboxyl fluorescein (FAM) and tetramethyl-6-carboxyrhodamine (TAMRA) were respectively selected as fluorophore of two MBs, Black Hole Quencher 1 (BHQ-1) and Black Hole Quencher 2 (BHQ-2) were respectively selected as organic quencher, and three continuous nucleotides with G base were connected to organic quencher (BHQ-1 and BHQ-2). In the presence of target DNAs, the two MBs hybridize with the corresponding target DNAs, the fluorophores are separated from organic quenchers and G bases, leading to recovery of fluorescence of FAM and TAMRA. Under a certain conditions, the fluorescence intensities of FAM and TAMRA all exhibited good linear dependence on their concentration of target DNAs (T1 and T2) in the range from 4 × 10 -10 to 4 × 10 -8 molL -1 (M). The detection limit (3σ, n = 13) of T1 was 3 × 10 -10 M and that of T2 was 2×10 -10 M, respectively. Compared with the existing analysis methods for multiplex DNA with MBs, this proposed method based on double quenching MBs is not only low fluorescence background, short analytical time and low detection cost, but also easy synthesis and good stability of MB probes. Copyright © 2017 Elsevier B.V. All rights reserved.

  13. A novel enzyme-linked immunosorbent assay for detection of Escherichia coli O157:H7 using immunomagnetic and beacon gold nanoparticles

    Science.gov (United States)

    2014-01-01

    This paper presents a functional nanoparticle-enhanced enzyme-linked immunosorbent assay (FNP-ELISA) for detection of enterohemorrhagic Escherichia coli (EHEC) O157:H7. Immunomagnetic nanoparticles (IMMPs) conjugated with monoclonal anti-O157:H7 antibody were used to capture E. coli O157:H7. Beacon gold nanoparticles (B-GNPs) coated with polyclonal anti-O157:H7 and biotin single-stranded DNA (B-DNA) were then subjective to immunoreaction with E. coli O157:H7, which was followed by streptavidin-horseradish peroxidase (Strep-HRP) conjugated with B-GNPs based on a biotin-avidin system. The solutions containing E. coli O157:H7, IMMPs, B-GNPs, and Strep-HRP were collected for detecting color change. The signal was significantly amplified with detection limits of 68 CFU mL-1 in PBS and 6.8 × 102 to 6.8 × 103 CFU mL-1 in the food samples. The FNP-ELISA method developed in this study was two orders of magnitude more sensitive than immunomagnetic separation ELISA (IMS-ELISA) and four orders of magnitude more sensitive than C-ELISA. The entire detection process of E. coli O157:H7 lasted only 3 h, and thus FNP-ELISA is considered as a time-saving method. PMID:24864164

  14. Continuous monitoring of restriction endonuclease cleavage activity by universal molecular beacon light quenching coupled with real-time polymerase chain reaction.

    Science.gov (United States)

    Li, Xiaomin; Song, Chen; Zhao, Meiping; Li, Yuanzong

    2008-10-01

    We describe a method for sensitive monitoring of restriction endonuclease kinetics and activity by use of a universal molecular beacon (U-MB) coupled with real-time polymerase chain reaction (PCR). The method is used to monitor the progress of DNA cleavage in a sealed reaction tube and offers more accurate and high-throughput detection. The template has a universal tail hybridized with the U-MB and the remaining sequence is complementary to one of the restriction endonuclease digestion products. The U-MB is replaced by the extension of digested product and the fluorescence quenches. With this concept, one universal fluorescence probe can be used in different enzyme analytical systems. In the work described here, homogenous assays were performed with the restriction endonucleases AluI, EcoRI, XhoI, and SacI at smoothly controlled temperature. Cleavage efficiencies were determined, and the potential applications of this method were discussed. Furthermore, the AluI and EcoRI cleavage reactions were monitored online at varying substrate concentrations at the molecular level, and K(m), V(max), and K(cat) values were calculated. The results suggest that U-MB monitoring of restriction endonuclease assays based on real-time PCR will be very useful for high-throughput, sensitive, and precise assays for enzyme activity screening and evolutionary biotechnology analysis.

  15. Effect of a Dual Charge on the DNA-Conjugated Redox Probe on DNA Sensing by Short Hairpin Beacons Tethered to Gold Electrodes.

    Science.gov (United States)

    Kékedy-Nagy, László; Shipovskov, Stepan; Ferapontova, Elena E

    2016-08-16

    Charges of redox species can critically affect both the interfacial state of DNA and electrochemistry of DNA-conjugated redox labels and, as a result, the electroanalytical performance of those systems. Here, we show that the kinetics of electron transfer (ET) between the gold electrode and methylene blue (MB) label conjugated to a double-stranded (ds) DNA tethered to gold strongly depend on the charge of the MB molecule, and that affects the performance of genosensors exploiting MB-labeled hairpin DNA beacons. Positively charged MB binds to dsDNA via electrostatic and intercalative/groove binding, and this binding allows the DNA-mediated electrochemistry of MB intercalated into the duplex and, as a result, a complex mode of the electrochemical signal change upon hairpin hybridization to the target DNA, dominated by the "on-off" signal change mode at nanomolar levels of the analyzed DNA. When MB bears an additional carboxylic group, the negative charge provided by this group prevents intimate interactions between MB and DNA, and then the ET in duplexes is limited by the diffusion of the MB-conjugated dsDNA (the phenomenon first shown in Farjami , E. ; Clima , L. ; Gothelf , K. ; Ferapontova , E. E. Anal. Chem. 2011 , 83 , 1594 ) providing the robust "off-on" nanomolar DNA sensing. Those results can be extended to other intercalating redox probes and are of strategic importance for design and development of electrochemical hybridization sensors exploiting DNA nanoswitchable architectures.

  16. SOX2 and OCT4 mRNA-expressing cells, detected by molecular beacons, localize to the center of neurospheres during differentiation.

    Directory of Open Access Journals (Sweden)

    Mirolyuba Ilieva

    Full Text Available Neurospheres are used as in vitro assay to measure the properties of neural stem cells. To investigate the molecular and phenotypic heterogeneity of neurospheres, molecular beacons (MBs targeted against the stem cell markers OCT4 and SOX2 were designed, and synthesized with a 2'-O-methyl RNA backbone. OCT4 and SOX2 MBs were transfected into human embryonic mesencephalon derived cells, which spontaneously form neurospheres when grown on poly-L-ornitine/fibronectin matrix and medium complemented with bFGF. OCT4 and SOX2 gene expression were tracked in individual cell using the MBs. Quantitative image analysis every day for seven days showed that the OCT4 and SOX2 mRNA-expressing cells clustered in the centre of the neurospheres cultured in differentiation medium. By contrast, cells at the periphery of the differentiating spheres developed neurite outgrowths and expressed the tyrosine hydroxylase protein, indicating terminal differentiation. Neurospheres cultured in growth medium contained OCT4 and SOX2-positive cells distributed throughout the entire sphere, and no differentiating neurones. Gene expression of SOX2 and OCT4 mRNA detected by MBs correlated well with gene and protein expression measured by qRT-PCR and immunostaining, respectively. These experimental data support the theoretical model that stem cells cluster in the centre of neurospheres, and demonstrate the use of MBs for the spatial localization of specific gene-expressing cells within heterogeneous cell populations.

  17. Real-time PCR with molecular beacons provides a highly accurate assay for detection of Tay-Sachs alleles in single cells.

    Science.gov (United States)

    Rice, John E; Sanchez, J Aquiles; Pierce, Kenneth E; Wangh, Lawrence J

    2002-12-01

    The results presented here provide the first single-cell genetic assay for Tay-Sachs disease based on real-time PCR. Individual lymphoblasts were lysed with an optimized lysis buffer and assayed using one pair of primers that amplifies both the wild type and 1278 + TATC Tay-Sachs alleles. The resulting amplicons were detected in real time with two molecular beacons each with a different colored fluorochrome. The kinetics of amplicon accumulation generate objective criteria by which to evaluate the validity of each reaction. The assay had an overall utility of 95%, based on the detection of at least one signal in 235 of the 248 attempted tests and an efficiency of 97%, as 7 of the 235 samples were excluded from further analysis for objective quantitative reasons. The accuracy of the assay was 99.1%, because 228 of 230 samples gave signals consistent with the genotype of the cells. Only two of the 135 heterozygous samples were allele drop-outs, a rate far lower than previously reported for single-cell Tay-Sachs assays using conventional methods of PCR. Copyright 2002 John Wiley & Sons, Ltd.

  18. MiRNA-Related SNPs and Risk of Esophageal Adenocarcinoma and Barrett's Esophagus: Post Genome-Wide Association Analysis in the BEACON Consortium.

    Directory of Open Access Journals (Sweden)

    Matthew F Buas

    Full Text Available Incidence of esophageal adenocarcinoma (EA has increased substantially in recent decades. Multiple risk factors have been identified for EA and its precursor, Barrett's esophagus (BE, such as reflux, European ancestry, male sex, obesity, and tobacco smoking, and several germline genetic variants were recently associated with disease risk. Using data from the Barrett's and Esophageal Adenocarcinoma Consortium (BEACON genome-wide association study (GWAS of 2,515 EA cases, 3,295 BE cases, and 3,207 controls, we examined single nucleotide polymorphisms (SNPs that potentially affect the biogenesis or biological activity of microRNAs (miRNAs, small non-coding RNAs implicated in post-transcriptional gene regulation, and deregulated in many cancers, including EA. Polymorphisms in three classes of genes were examined for association with risk of EA or BE: miRNA biogenesis genes (157 SNPs, 21 genes; miRNA gene loci (234 SNPs, 210 genes; and miRNA-targeted mRNAs (177 SNPs, 158 genes. Nominal associations (P0.50, and we did not find evidence for interactions between variants analyzed and two risk factors for EA/BE (smoking and obesity. This analysis provides the most extensive assessment to date of miRNA-related SNPs in relation to risk of EA and BE. While common genetic variants within components of the miRNA biogenesis core pathway appear unlikely to modulate susceptibility to EA or BE, further studies may be warranted to examine potential associations between unassessed variants in miRNA genes and targets with disease risk.

  19. BlueDetect: An iBeacon-Enabled Scheme for Accurate and Energy-Efficient Indoor-Outdoor Detection and Seamless Location-Based Service

    Directory of Open Access Journals (Sweden)

    Han Zou

    2016-02-01

    Full Text Available The location and contextual status (indoor or outdoor is fundamental and critical information for upper-layer applications, such as activity recognition and location-based services (LBS for individuals. In addition, optimizations of building management systems (BMS, such as the pre-cooling or heating process of the air-conditioning system according to the human traffic entering or exiting a building, can utilize the information, as well. The emerging mobile devices, which are equipped with various sensors, become a feasible and flexible platform to perform indoor-outdoor (IO detection. However, power-hungry sensors, such as GPS and WiFi, should be used with caution due to the constrained battery storage on mobile device. We propose BlueDetect: an accurate, fast response and energy-efficient scheme for IO detection and seamless LBS running on the mobile device based on the emerging low-power iBeacon technology. By leveraging the on-broad Bluetooth module and our proposed algorithms, BlueDetect provides a precise IO detection service that can turn on/off on-board power-hungry sensors smartly and automatically, optimize their performances and reduce the power consumption of mobile devices simultaneously. Moreover, seamless positioning and navigation services can be realized by it, especially in a semi-outdoor environment, which cannot be achieved by GPS or an indoor positioning system (IPS easily. We prototype BlueDetect on Android mobile devices and evaluate its performance comprehensively. The experimental results have validated the superiority of BlueDetect in terms of IO detection accuracy, localization accuracy and energy consumption.

  20. BlueDetect: An iBeacon-Enabled Scheme for Accurate and Energy-Efficient Indoor-Outdoor Detection and Seamless Location-Based Service.

    Science.gov (United States)

    Zou, Han; Jiang, Hao; Luo, Yiwen; Zhu, Jianjie; Lu, Xiaoxuan; Xie, Lihua

    2016-02-22

    The location and contextual status (indoor or outdoor) is fundamental and critical information for upper-layer applications, such as activity recognition and location-based services (LBS) for individuals. In addition, optimizations of building management systems (BMS), such as the pre-cooling or heating process of the air-conditioning system according to the human traffic entering or exiting a building, can utilize the information, as well. The emerging mobile devices, which are equipped with various sensors, become a feasible and flexible platform to perform indoor-outdoor (IO) detection. However, power-hungry sensors, such as GPS and WiFi, should be used with caution due to the constrained battery storage on mobile device. We propose BlueDetect: an accurate, fast response and energy-efficient scheme for IO detection and seamless LBS running on the mobile device based on the emerging low-power iBeacon technology. By leveraging the on-broad Bluetooth module and our proposed algorithms, BlueDetect provides a precise IO detection service that can turn on/off on-board power-hungry sensors smartly and automatically, optimize their performances and reduce the power consumption of mobile devices simultaneously. Moreover, seamless positioning and navigation services can be realized by it, especially in a semi-outdoor environment, which cannot be achieved by GPS or an indoor positioning system (IPS) easily. We prototype BlueDetect on Android mobile devices and evaluate its performance comprehensively. The experimental results have validated the superiority of BlueDetect in terms of IO detection accuracy, localization accuracy and energy consumption.

  1. A modified molecular beacons-based multiplex real-time PCR assay for simultaneous detection of eight foodborne pathogens in a single reaction and its application.

    Science.gov (United States)

    Hu, Qinghua; Lyu, Dongyue; Shi, Xiaolu; Jiang, Yixiang; Lin, Yiman; Li, Yinghui; Qiu, Yaqun; He, Lianhua; Zhang, Ran; Li, Qingge

    2014-03-01

    Foodborne disease outbreaks are often caused by one of the major pathogens. Early identification of the causal pathogen is crucial for disease control and prevention. We describe a real-time polymerase chain reaction (rtPCR) assay that can identify, in a single reaction, up to eight common foodborne bacterial pathogens, including Salmonella enterica subsp. enterica, Listeria monocytogenes, Escherichia coli O157, Vibrio parahaemolyticus, V. vulnificus, Campylobacter jejuni, Enterobacter sakazakii, and Shigella spp. This multiplex rtPCR assay takes advantage of modified molecular beacons and the multicolor combinational probe coding strategy to discriminate each pathogen and the homo-tag assisted non-dimer (HAND) system to prevent dimer formation. The detection limits of the assay ranged from 1.3×10(3) colony-forming units (CFU)/g stool (L. monocytogenes) to 1.6×10(4) CFU/g stool (Shigella spp.). The target genes were 100% specific as assessed on 986 reference strains covering 41 species since no cross-reactions were observed. The assay was applied to the detection of foodborne pathogens in 11,167 clinical samples and the results were compared with culture methods for further validation. The sensitivity and specificity of the rtPCR were 100% and 99%, respectively. When performed in a 96-well rtPCR system, more than 90 samples could be analyzed within 3 h. Given the high accuracy, sensitivity, specificity, and short turn-around time, the established assay could be used for the rapid and reliable identification of the causative pathogens responsible for a certain foodborne disease outbreak and rapid screening of these major foodborne pathogens in laboratory-based surveillance of outpatient clinical samples or even food samples.

  2. Description and analysis of assistance in science teaching reform: The reports of Maine's Beacon Center science facilitators and the teachers they help

    Science.gov (United States)

    Kesselheim, Craig

    This study describes the assistance relationship between teachers engaged in school-based science education reform and the full-time facilitators hired to provide their training and support. The model which supplied these facilitators to Maine's "Beacon Center" schools was unique in combining three important design factors: content expertise, the duration of their time on site, and the intensity of the assistance provided. Participant perceptions regarding the usefulness of facilitators' assistance were studied, as were the reported impacts of facilitators' assistance on science teaching practices. Participants in the study were three science facilitators and fourteen teachers. Interviewing was the primary method of data gathering, although daily logs from facilitators also provided essential information. Seven categories of assistance were indicated by participants. Among these were several which assisted teachers' efforts to deliver innovative practices in classroom science. Other categories included one which added to teachers' capacities and one which promoted and guided overall efforts. The seven categories occurred at all three sites, although there were unique patterns in the frequency with which each kind occurred at a site. Factors were described at each site which appear to have affected the forms of assistance which facilitators provided. Teacher perceptions of what made facilitator assistance useful were grouped into two broad categories. Assistance was most useful when it possessed a feature of immediate application or "implementability." Another perception of teachers' related to professional growth value of assistance, including such areas as confidence and content knowledge. Three impacts of facilitator assistance on teachers' practice were also reported in this study: More time spent on science; shifts in science teaching philosophy or instructional preferences; participation in systems-level changes in science teaching. The implications of this

  3. Species detection using HyBeacon(®) probe technology: Working towards rapid onsite testing in non-human forensic and food authentication applications.

    Science.gov (United States)

    Dawnay, Nick; Hughes, Rebecca; Court, Denise Syndercombe; Duxbury, Nicola

    2016-01-01

    Identifying individual species or determining species' composition in an unknown sample is important for a variety of forensic applications. Food authentication, monitoring illegal trade in endangered species, forensic entomology, sexual assault case work and counter terrorism are just some of the fields that can require the detection of the biological species present. Traditional laboratory based approaches employ a wide variety of tools and technologies and exploit a number of different species specific traits including morphology, molecular differences and immuno-chemical analyses. A large number of these approaches require laboratory based apparatus and results can take a number of days to be returned to investigating authorities. Having a presumptive test for rapid identification could lead to savings in terms of cost and time and allow sample prioritisation if confirmatory testing in a laboratory is required later. This model study describes the development of an assay using a single HyBeacon(®) probe and melt curve analyses allowing rapid screening and authentication of food products labelled as Atlantic cod (Gadus morhua). Exploiting melt curve detection of species specific SNP sites on the COI gene the test allows detection of a target species (Atlantic cod) and closely related species which may be used as substitutes. The assay has been designed for use with the Field Portable ParaDNA system, a molecular detection platform for non-expert users. The entire process from sampling to result takes approximately 75min. Validation studies were performed on both single source genomic DNA, mixed genomic DNA and commercial samples. Data suggests the assay has a lower limit of detection of 31 pg DNA. The specificity of the assay to Atlantic cod was measured by testing highly processed food samples including frozen, defrosted and cooked fish fillets as well as fish fingers, battered fish fillet and fish pie. Ninety-six (92.7%) of all Atlantic cod food products

  4. The ANTARES optical beacon system

    NARCIS (Netherlands)

    Ageron, M.; Aguilar, J. A.; Albert, A.; Ameli, F.; Anghinolfi, M.; Anton, G.; Anvar, S.; Ardellier-Desages, F.; Aslanides, E.; Aubert, J-J.; Auer, R.; Barbarito, E.; Basa, S.; Battaglieri, M.; Becherini, Y.; Beltramelli, J.; Bertin, V.; Bigi, A.; Billault, M.; Blaes, R.; de Botton, N.; Bradbury, S. M.; Bruijn, R.; Brunner, J.; Burgio, G. F.; Busto, J.; Cafagna, F.; Caillat, L.; Calzas, A.; Capone, A.; Caponetto, L.; Carmona, E.; Carr, J.; Cartwright, S. L.; Castel, D.; Castorina, E.; Cavasinni, V.; Cecchini, S.; Ceres, A.; Charvis, P.; Chauchot, P.; Chiarusi, T.; Circella, M.; Colnard, C.; Compere, C.; Coniglione, R.; Cottini, N.; Coyle, P.; Cuneo, S.; Cussatlegras, A-S.; Damy, G.; van Dantzig, R.; De Bonis, G.; De Marzo, C.; De Vita, R.; Dekeyser, I.; Delagnes, E.; Denans, D.; Deschamps, A.; Destelle, J-J.; Dinkespieler, B.; Distefano, C.; Donzaud, C.; Drogou, J-F.; Druillole, F.; Durand, D.; Ernenwein, J-P.; Escoffier, S.; Falchini, E.; Favard, S.; Fehr, F.; Feinstein, F.; Ferry, S.; Fiorello, C.; Flaminio, V.; Fratini, K.; Fuda, J-L.; Galeotti, S.; Gallone, J-M.; Giacomelli, G.; Girard, N.; Gojak, C.; Goret, Ph.; Graf, K.; Hallewell, G.; Harakeh, M. N.; Hartmann, B.; Heijboer, A.; Heine, E.; Hello, Y.; Hernandez-Rey, J. J.; Hoessl, J.; Hoffman, C.; Hogenbirk, J.; Hubbard, J. R.; Jaquet, M.; de Jong, M.; Jouvenot, F.; Kalantar-Nayestanaki, N.; Kappes, A.; Karg, T.; Katz, U.; Keller, P.; Kok, E.; Kok, H.; Kooijman, P.; Kopper, C.; Korolkova, E. V.; Kouchner, A.; Kretschmer, W.; Kruijer, A.; Kuch, S.; Kudryavstev, V. A.; Lagier, P.; Lahmann, R.; Lamanna, G.; Lamare, P.; Lambard, G.; Languillat, J-C.; Laschinsky, H.; Lavalle, J.; Le Guen, Y.; Le Provost, H.; Van Suu, A. Le; Lefevre, D.; Legou, T.; Lelaizant, G.; Lim, G.; Lo Presti, D.; Löhner, H.; Loucatos, S.; Louis, F.; Lucarelli, F.; Lyashuk, V.; Marcelin, M.; Margiotta, A.; Masullo, R.; Mazeas, F.; Mazure, A.; McMillan, J. E.; Megna, R.; Melissas, M.; Migneco, E.; Milovanovic, A.; Mongelli, M.; Montaruli, T.; Morganti, M.; Moscoso, L.; Musumeci, M.; Naumann-Godo, M.; Naumann, C.; Niess, V.; Noble, T.; Olivetto, C.; Ostasch, R.; Palanque-Delabrouille, N.; Payre, P.; Peek, H.; Perez, A.; Petta, C.; Piattelli, P.; Pillet, R.; Pineau, J-P.; Poinsignon, J.; Popa, V.; Pradier, T.; Racca, C.; Randazzo, N.; van Randwijk, J.; Real, D.; van Rens, B.; Rethore, F.; Rewiersma, P.; Riccobene, G.; Rigaud, V.; Ripani, M.; Roca, V.; Roda, C.; Rolin, J. F.; Rose, H. J.; Rostovtsev, A.; Roux, J.; Ruppi, M.; Russo, G. V.; Rusydi, G.; Salesa, F.; Salomon, K.; Sapienza, P.; Schmitt, F.; Schuller, J-P.; Shanidze, R.; Sokalski, I.; Spona, T.; Spurio, M.; van der Steenhoven, G.; Stolarczyk, T.; Streeb, K.; Sulak, L.; Taiuti, M.; Tamburini, C.; Tao, C.; Terreni, G.; Thompson, L. F.; Urbano, F.; Valdy, P.; Valente, V.; Vallage, B.; Vaudaine, G.; Venekamp, G.; Verlaat, B.; Vernin, P.; de Vries-Uiterweerd, G.; Wijnker, G.; Huberts, P. de Witt; Wobbe, G.; de Wolf, E.; Yao, A-F.; Zaborov, D.; Zaccone, H.; Zornoza, J. D.; Zuniga, J.; van Wijk, R.

    2007-01-01

    ANTARES is a neutrino telescope being deployed in the Mediterranean Sea. It consists of a three-dimensional array of photomultiplier tubes that can detect the Cherenkov light induced by charged particles produced in the interactions of neutrinos with the surrounding medium. High angular resolution

  5. Underwater Acoustic Beacon Location System

    Science.gov (United States)

    2016-12-23

    distance is the predicted detection range R based on consideration of Equations (1) and (2) together with the parameters for SL, NL, DI, DT and alpha ...R nvnv nvnv ia               cossin0 sincos0 0012 (12) [0091] Finally, the positions on the seafloor 500 in the array...numerical values of the beam pattern projected onto the seafloor are divided by the sum of all of the (non- zero ) numerical values projected onto the

  6. Beacons of the Experience Economy

    DEFF Research Database (Denmark)

    Delica, Kristian Nagel

    2014-01-01

    This essay outlines the boundaries among various interests in the library sector; among positions that prioritize libraries as aesthetically appealing junctions of the experience economy versus positions that prioritize the library as a community forum. The main emphasis is placed on developments...... in the Danish library sector with the additional inclusion of international examples....

  7. Health-related quality of life in patients with locally recurrent or metastatic breast cancer treated with etirinotecan pegol versus treatment of physician's choice: Results from the randomised phase III BEACON trial.

    Science.gov (United States)

    Twelves, Chris; Cortés, Javier; O'Shaughnessy, Joyce; Awada, Ahmad; Perez, Edith A; Im, Seock-Ah; Gómez-Pardo, Patricia; Schwartzberg, Lee S; Diéras, Véronique; Yardley, Denise A; Potter, David A; Mailliez, Audrey; Moreno-Aspitia, Alvaro; Ahn, Jin-Seok; Zhao, Carol; Hoch, Ute; Tagliaferri, Mary; Hannah, Alison L; Rugo, Hope S

    2017-05-01

    Health-related quality of life (HRQoL) enhances understanding of treatment effects that impact clinical decision-making. Although the primary end-point was not achieved, the BEACON (BrEAst Cancer Outcomes with NKTR-102) trial established etirinotecan pegol, a long-acting topoisomerase-1 (TOP1) inhibitor, as a promising therapeutic for patients with advanced/metastatic breast cancer (MBC) achieving clinically meaningful benefits in median overall survival (OS) for patients with stable brain metastases, with liver metastases or ≥ 2 sites of metastatic disease compared to treatment of physician's choice (TPC). Reported herein are the findings from the preplanned secondary end-point of HRQoL. HRQoL, assessed by European Organisation for Research and Treatment of Cancer (EORTC) Quality of Life Questionnaire-Core 30 (QLQ-C30) (version 3.0) supplemented by the breast cancer-specific Quality of Life Questionnaire (QLQ-BR23), was evaluated post randomisation in 733 of 852 patients with either anthracycline-, taxane- and capecitabine-pretreated locally recurrent or MBC randomised to etirinotecan pegol (n = 378; 145 mg/m 2 every 3 weeks (q3wk)) or single-agent TPC (n = 355). Patients completed assessments at screening, every 8 weeks (q8wk) during treatment, and end-of-treatment. Changes from baseline were analysed, and the proportions of patients achieving differences (≥5 points) in HRQoL scores were compared. Differences were seen favouring etirinotecan pegol up to 32 weeks for global health status (GHS) and physical functioning scales (P adverse event profiles; etirinotecan pegol was associated with worsening gastrointestinal symptoms whereas TPC was associated with worsened dyspnoea and other systemic side-effects. Analysis of GHS and physical functioning at disease progression showed a decline in HRQoL in both treatment arms, with a mean change from baseline of -9.4 and -10.8 points, respectively. There was evidence of benefit associated with etirinotecan

  8. Infrared Beacons based Localization of Mobile Robot

    Czech Academy of Sciences Publication Activity Database

    Krejsa, Jiří; Věchet, Stanislav

    2012-01-01

    Roč. 117, č. 1 (2012), s. 17-22 ISSN 1392-1215 Institutional research plan: CEZ:AV0Z20760514 Keywords : mobile robot localization * extended Kalman filter * autonomous mobile robot Subject RIV: JD - Computer Applications, Robot ics Impact factor: 0.411, year: 2012 http://www.eejournal.ktu.lt/index.php/elt/article/view/1046

  9. Dynamic Bluetooth beacons for people with disabilities

    DEFF Research Database (Denmark)

    Alapetite, Alexandre; Hansen, John Paulin

    This paper focuses on digital aids for sight impairment and motor disabilities. We propose an Internet of Things (IoT) platform for discovering nearby items, getting their status, and interacting with them by e.g. voice commands or gaze gestures. The technology is based on Bluetooth Low Energy...

  10. Passive Localization of Underwater Acoustic Beacons

    Science.gov (United States)

    1993-09-01

    maxtime) TickCounto; aborto ; FSRead(gASerRefln,&numCharln,&addressBuf); TalkSail(’O); iistart the PTR’s data collecticn DrawString(w\\p: H) I display a...34); aborto ; EventLoopO; II main execution loop RAMSDClose(sPortA); HI must close the RAM Drivers before shutdown 193 free(gw); //must release the...kNumExp+l),sizeof(double)); if(wtrue ==NULL) DrawString("\\pCan’t allocate memory for data collection!!! ); aborto ; Nvmeas = calloc(3L*(kStep~kNumExp+l

  11. iBeacons: context awareness and microlocation

    OpenAIRE

    Jiménez Cancho, Daniel

    2015-01-01

    New technologies are developed and introduced to the market every day, shaping people's daily life. More powerful and efficient devices, richer services and high-speed connections are the key ingredients that make up an ecosystem that provides anything, anytime, anywhere. Bluetooth cannot be considered a brand-new technology - it has been around for some time wirelessly connecting nearby gadgets - but it has notably evolved and increased popularity due to a hot topic in rece...

  12. Dynamic Bluetooth beacons for people with disabilities

    DEFF Research Database (Denmark)

    Alapetite, Alexandre; Hansen, John Paulin

    2017-01-01

    This paper focuses on digital aids for sight impairment and motor disabilities. We propose an Internet of Things (IoT) platform for discovering nearby items, getting their status, and interacting with them by e.g. voice commands or gaze gestures. The technology is based on Bluetooth Low Energy...

  13. Contention Window Analysis for Beaconing in VANETs

    NARCIS (Netherlands)

    Reinders, René; van Eenennaam, Martijn; Karagiannis, Georgios; Heijenk, Geert

    2011-01-01

    The recently standardised IEEE 802.11p Wireless Access in Vehicular Environment (WAVE) protocol is envisioned to be used for Vehicle-to-Vehicle and Vehicle-to-Infrastructure communications. We provide an analysis of 802.11p’s performance when used for the exchange of small status messages known as

  14. BEACON : A Summary Framework to Overcome Potential Reimbursement Hurdles

    NARCIS (Netherlands)

    Dunlop, William C. N.; Mullins, C. Daniel; Pirk, Olaf; Goeree, Ron; Postma, Maarten J.; Enstone, Ashley; Heron, Louise

    2016-01-01

    Objective To provide a framework for addressing payers' criteria during the development of pharmaceuticals. Methods A conceptual framework was presented to an international health economic expert panel for discussion. A structured literature search (from 2010 to May 2015), using the following

  15. Opportunistic Beacon Networks: Information Dissemination via Wireless Network Identifiers

    NARCIS (Netherlands)

    Türkes, Okan; Scholten, Johan; Havinga, Paul J.M.

    2016-01-01

    This paper presents OBN, a universal opportunistic ad hoc networking model particularly intended for smart mobile devices. It enables fast and lightweight data dissemination in wireless community networks through the utilization of universally-available wireless network identifiers. As a ubiquitous

  16. Time-Synchronized Beacons for Relative UAV Position Determination

    Data.gov (United States)

    National Aeronautics and Space Administration — Research is required to determine the optimal pulse length, pulse frequency, pulse power, and optical communications encoding technique to meet FAA and STMD...

  17. Early Detection of Breast Cancer Using Molecular Beacons

    Science.gov (United States)

    2008-01-01

    DCIS tissues (Figure 6). Therefore, in addition to three tumor markers that were originally proposed for this study, we added HIF-1α and uPAR as...Ambrosini, G., Adida , C., and Altieri, D. C. (1997) Nat. Med. 3, 917–921 17. Tanaka, K., Iwamoto, S., Gon, G., Nohara, T., Iwamoto,M., andTanigawa, N...lab will only be given the ID number of each sample. All research results will be recorded using the ID numbers. The original experimental records

  18. Detection of Periodic Beacon Loads in Electrical Distribution Substation Data

    Energy Technology Data Exchange (ETDEWEB)

    Hammerstrom, Donald J.; Guttromson, Ross T.; Lu, Ning; Boyd, Paul A.; Trudnowski, Daniel; Chassin, David P.; Bonebrake, Christopher A.; Shaw, James M.

    2006-05-31

    This research explores methods for identifying a whether a load is sending a signal to the utility SCADA system. Such a system can identify whether various loads are signialing using existing SCADA infrastructure, that is, without added, high cost communications infrastructure.

  19. Early Detection of Breast Cancer Using Molecular Beacons

    National Research Council Canada - National Science Library

    Yang, Lily

    2004-01-01

    .... We also developed a procedure to detect both survivin and cyclin Dl gene expression simultaneously in single cancer cells, which increases the sensitivity and specificity of detection of cancer cells...

  20. Doing Forensic on DTUsat-2 Using the Beacon Counter

    DEFF Research Database (Denmark)

    Fléron, René

    2016-01-01

    rises from the south. That observation is in direct conflict with our model for DTUsat-2 attitude. The electrical power subsystem became the primary suspect in the search for a cause for the frequent resets. However a fault in the power subsystem could not explain why the resets would occur just before...

  1. Indoor self-localization via bluetooth low energy beacons

    NARCIS (Netherlands)

    Rizky Pratama, Azkario; Widyawan, Widyawan; Lazovik, Aliaksandr; Aiello, Marco

    2017-01-01

    Indoor localization is concerned with mapping sensory data to physical locations inside buildings. Location of a user or a mobile device is an essential part of the context, and is therefore very useful for pervasive computing applications. Many proposals exist for solving the localization problem,

  2. Close-Range Tracking of Underwater Vehicles Using Light Beacons

    Science.gov (United States)

    Bosch, Josep; Gracias, Nuno; Ridao, Pere; Istenič, Klemen; Ribas, David

    2016-01-01

    This paper presents a new tracking system for autonomous underwater vehicles (AUVs) navigating in a close formation, based on computer vision and the use of active light markers. While acoustic localization can be very effective from medium to long distances, it is not so advantageous in short distances when the safety of the vehicles requires higher accuracy and update rates. The proposed system allows the estimation of the pose of a target vehicle at short ranges, with high accuracy and execution speed. To extend the field of view, an omnidirectional camera is used. This camera provides a full coverage of the lower hemisphere and enables the concurrent tracking of multiple vehicles in different positions. The system was evaluated in real sea conditions by tracking vehicles in mapping missions, where it demonstrated robust operation during extended periods of time. PMID:27023547

  3. Proteomics in reproductive biology: beacon for unraveling the molecular complexities.

    Science.gov (United States)

    Upadhyay, Rahul D; Balasinor, N H; Kumar, Anita V; Sachdeva, Geetanjali; Parte, Priyanka; Dumasia, Kushaan

    2013-01-01

    Proteomics, an interface of rapidly evolving advances in physics and biology, is rapidly developing and expanding its potential applications to molecular and cellular biology. Application of proteomics tools has contributed towards identification of relevant protein biomarkers that can potentially change the strategies for early diagnosis and treatment of several diseases. The emergence of powerful mass spectrometry-based proteomics technique has added a new dimension to the field of medical research in liver, heart diseases and certain forms of cancer. Most proteomics tools are also being used to study physiological and pathological events related to reproductive biology. There have been attempts to generate the proteomes of testes, sperm, seminal fluid, epididymis, oocyte, and endometrium from reproductive disease patients. Here, we have reviewed proteomics based investigations in humans over the last decade, which focus on delineating the mechanism underlying various reproductive events such as spermatogenesis, oogenesis, endometriosis, polycystic ovary syndrome, embryo development. The challenge is to harness new technologies like 2-DE, DIGE, MALDI-MS, SELDI-MS, MUDPIT, LC-MS etc., to a greater extent to develop widely applicable clinical tools in understanding molecular aspects of reproduction both in health and disease. Copyright © 2012 Elsevier B.V. All rights reserved.

  4. Complementary terrain/single beacon-based AUV navigation

    Digital Repository Service at National Institute of Oceanography (India)

    Maurya, P.; Curado, T.F.; António, P.

    is not sufficiently "rich" in terms of topographic features. The key contribution of this paper is a formal analysis of the benefits of using complementary filtering, in opposition to TAN navigation only. To this effect, we exploit key tools of estimation theory...

  5. Beacons in the limnological landscape | Allanson | African Journal of ...

    African Journals Online (AJOL)

    An overview is given of a number of major developments in the limnology of South Africa, Zimbabwe and Namibia. None was possible without the involvement of enthusiastic investigators who, over some 70 years, stimulated the growth of freshwater research in these arid lands. The contributions to the nascent limnology of ...

  6. Improving Indoor Localization Using Bluetooth Low Energy Beacons

    Directory of Open Access Journals (Sweden)

    Pavel Kriz

    2016-01-01

    Full Text Available The paper describes basic principles of a radio-based indoor localization and focuses on the improvement of its results with the aid of a new Bluetooth Low Energy technology. The advantage of this technology lies in its support by contemporary mobile devices, especially by smartphones and tablets. We have implemented a distributed system for collecting radio fingerprints by mobile devices with the Android operating system. This system enables volunteers to create radio-maps and update them continuously. New Bluetooth Low Energy transmitters (Apple uses its “iBeacon” brand name for these devices have been installed on the floor of the building in addition to existing WiFi access points. The localization of stationary objects based on WiFi, Bluetooth Low Energy, and their combination has been evaluated using the data measured during the experiment in the building. Several configurations of the transmitters’ arrangement, several ways of combination of the data from both technologies, and other parameters influencing the accuracy of the stationary localization have been tested.

  7. Exploring the Transformative Potential of Bluetooth Beacons in Higher Education

    Science.gov (United States)

    McDonald, Kieran; Glover, Ian

    2016-01-01

    The growing ubiquity of smartphones and tablet devices integrated into personal, social and professional life, facilitated by expansive communication networks globally, has the potential to disrupt higher education. Academics and students are considering the future possibilities of exploiting these tools and utilising networks to consolidate and…

  8. Proceedings of the Symposium of the COSPAR Satellite Beacon Group on the Geophysical Use of Satellite Beacon Observations Held at Boston University on 1- 4 June 1976

    Science.gov (United States)

    1976-06-04

    tion to Radio Science, 1975 WeiB, W. Zwick, R. [3] Becker , H. "MEPOL Empfangsanlage" Engelhardt, W. MPI f. Aeronomie, Interner Bericht, Eulig, M.D...Berucksichti- gung der Effekte , die bei- Mitteilungen aus de. Max- Planck Institut fur Aoro- nomie•, Rep. No. 31(I). Hart-an, G. 1969 J. Atuosb. Terr

  9. In-Service Evaluation of the Dalmo Victor Active Beacon Collision Avoidance System (BCAS/TCAS).

    Science.gov (United States)

    1982-10-01

    to the flight crew. This techni- que requires continuous radar altitude information and operates only when the TCAS aircraft is less than 2500 feet...boxes 1. DC - in clouds majority of time 2. VMC - outside clouds 3. Marginal - officially VFR, but flight conditions (sun, haze, smog ) are marginal

  10. DOE Zero Energy Ready Home Case Study: United Way of Long Island, United Veterans Beacon House

    Energy Technology Data Exchange (ETDEWEB)

    Pacific Northwest National Laboratory

    2017-09-01

    United Way of Long Island’s Housing Development Corporation built this 3,719-ft2 two–story, 5-bedroom home in Huntington Station, New York, to the rigorous performance requirements of the U.S. Department of Energy’s Zero Energy Ready Home Program. The home is packed with high-performance features like LED lighting and ENERGY STAR appliances. The asymmetrical, optimally angled roof provides plenty of space for roof-mounted solar panels for electric generation and hot water.

  11. BLESSED with Opportunistic Beacons: A Lightweight Data Dissemination Model for Smart Mobile Ad-Hoc Networks

    NARCIS (Netherlands)

    Türkes, Okan; Scholten, Johan; Havinga, Paul J.M.

    2015-01-01

    This paper introduces BLESSED, a universal opportunistic ad hoc networking model intended for smart mobile devices. It enables fast and lightweight data dissemination in wireless community networks through the complementary utilization of the IEEE 802.11 and Bluetooth Low Energy standards. As a

  12. Physiological Monitoring of a Wrist-Worn Personal Locator Beacon User

    Directory of Open Access Journals (Sweden)

    Alejandro DE MIQUEL

    2016-11-01

    Full Text Available This paper presents the concept, developments and preliminary results of the Horizon 2020 project named SAT406M. The goal of this project is to develop an application based on a wrist-worn device, conceived to be a maritime application, and the use of European Global Navigation Satellite Systems, based on the Galileo Search and Rescue service, using its unique Return-Link-Message function, improving the mobility and safety of citizens. In particular for this paper, we focus on the development and the first testing and results of a physiological monitoring component included in the device. This component will provide the Search and Rescue services with additional information about the SAT406M user's physiological status once the distress alarm is triggered. The algorithm implemented uses some stochastic techniques to deduce the SAT406M user's physiological status, encoded in two bits, from sensor inputs. The results here presented, which are still preliminary results from an intermediate stage of the project, show a good accuracy in most of the cases. This makes us conclude that the algorithm has the potential to end up determining the SAT406M user's physiological status accurately enough, improving this way the user's safety.

  13. Fusing Bluetooth Beacon Data with Wi-Fi Radiomaps for Improved Indoor Localization

    Science.gov (United States)

    Kanaris, Loizos; Kokkinis, Akis; Liotta, Antonio; Stavrou, Stavros

    2017-01-01

    Indoor user localization and tracking are instrumental to a broad range of services and applications in the Internet of Things (IoT) and particularly in Body Sensor Networks (BSN) and Ambient Assisted Living (AAL) scenarios. Due to the widespread availability of IEEE 802.11, many localization platforms have been proposed, based on the Wi-Fi Received Signal Strength (RSS) indicator, using algorithms such as K-Nearest Neighbour (KNN), Maximum A Posteriori (MAP) and Minimum Mean Square Error (MMSE). In this paper, we introduce a hybrid method that combines the simplicity (and low cost) of Bluetooth Low Energy (BLE) and the popular 802.11 infrastructure, to improve the accuracy of indoor localization platforms. Building on KNN, we propose a new positioning algorithm (dubbed i-KNN) which is able to filter the initial fingerprint dataset (i.e., the radiomap), after considering the proximity of RSS fingerprints with respect to the BLE devices. In this way, i-KNN provides an optimised small subset of possible user locations, based on which it finally estimates the user position. The proposed methodology achieves fast positioning estimation due to the utilization of a fragment of the initial fingerprint dataset, while at the same time improves positioning accuracy by minimizing any calculation errors. PMID:28394268

  14. An Indoor Location-Based Control System Using Bluetooth Beacons for IoT Systems

    OpenAIRE

    Jun-Ho Huh; Kyungryong Seo

    2017-01-01

    The indoor location-based control system estimates the indoor position of a user to provide the service he/she requires. The major elements involved in the system are the localization server, service-provision client, user application positioning technology. The localization server controls access of terminal devices (e.g., Smart Phones and other wireless devices) to determine their locations within a specified space first and then the service-provision client initiates required services such...

  15. Beacon of Hope: Award-Winning Program Redesign for Post-Traditional Students

    Science.gov (United States)

    Cherrstrom, Catherine A.; Boden, Carrie J.

    2018-01-01

    Many Americans dream of completing a college degree, positively influencing wages and economic mobility. The number of students entering college or returning to college later in life is growing. Up to 75% of university enrollments are comprised of students in one or more of the following circumstances: 25 years old or older, attend school…

  16. Modeling Self-Referencing Interferometers with Extended Beacons and Strong Turbulence

    Science.gov (United States)

    2011-09-01

    transport theory [27], Markov approxi- mations [82], and Feynman path integrals [21]. It has been shown that to the second order when approximations similar...1983. [11] Belen’kii, Mikhail S., Jeff Barchers, Eric Bruns, Deborah Fung, Richard Gal- lant, Clay Kirk, Hope Runyeon, Vincent Rye, and Josh Voass...partially coherent beams through atmospheric turbulence”. Opt. Commun., 216:261–265, 2003. [77] Sasiela, Richard J. Electromagnetic Wave Propagation

  17. A Novel 3D Multilateration Sensor Using Distributed Ultrasonic Beacons for Indoor Navigation

    Directory of Open Access Journals (Sweden)

    Rohan Kapoor

    2016-10-01

    Full Text Available Navigation and guidance systems are a critical part of any autonomous vehicle. In this paper, a novel sensor grid using 40 KHz ultrasonic transmitters is presented for adoption in indoor 3D positioning applications. In the proposed technique, a vehicle measures the arrival time of incoming ultrasonic signals and calculates the position without broadcasting to the grid. This system allows for conducting silent or covert operations and can also be used for the simultaneous navigation of a large number of vehicles. The transmitters and receivers employed are first described. Transmission lobe patterns and receiver directionality determine the geometry of transmitter clusters. Range and accuracy of measurements dictate the number of sensors required to navigate in a given volume. Laboratory experiments were performed in which a small array of transmitters was set up and the sensor system was tested for position accuracy. The prototype system is shown to have a 1-sigma position error of about 16 cm, with errors between 7 and 11 cm in the local horizontal coordinates. This research work provides foundations for the future development of ultrasonic navigation sensors for a variety of autonomous vehicle applications.

  18. IR Beacon System for Assisted or Automated Landing of Aircraft, Phase I

    Data.gov (United States)

    National Aeronautics and Space Administration — OKSI proposes to design an optical system to support assisted or automated precision approach of fixed or rotary wing aircraft, or other low altitude airspace...

  19. An Indoor Location-Based Control System Using Bluetooth Beacons for IoT Systems.

    Science.gov (United States)

    Huh, Jun-Ho; Seo, Kyungryong

    2017-12-19

    The indoor location-based control system estimates the indoor position of a user to provide the service he/she requires. The major elements involved in the system are the localization server, service-provision client, user application positioning technology. The localization server controls access of terminal devices (e.g., Smart Phones and other wireless devices) to determine their locations within a specified space first and then the service-provision client initiates required services such as indoor navigation and monitoring/surveillance. The user application provides necessary data to let the server to localize the devices or allow the user to receive various services from the client. The major technological elements involved in this system are indoor space partition method, Bluetooth 4.0, RSSI (Received Signal Strength Indication) and trilateration. The system also employs the BLE communication technology when determining the position of the user in an indoor space. The position information obtained is then used to control a specific device(s). These technologies are fundamental in achieving a "Smart Living". An indoor location-based control system that provides services by estimating user's indoor locations has been implemented in this study (First scenario). The algorithm introduced in this study (Second scenario) is effective in extracting valid samples from the RSSI dataset but has it has some drawbacks as well. Although we used a range-average algorithm that measures the shortest distance, there are some limitations because the measurement results depend on the sample size and the sample efficiency depends on sampling speeds and environmental changes. However, the Bluetooth system can be implemented at a relatively low cost so that once the problem of precision is solved, it can be applied to various fields.

  20. Molecular beacon nanosensors for live cell detection and tracking differentiation and reprogramming

    DEFF Research Database (Denmark)

    Ilieva, Mirolyuba

    2013-01-01

    High-sensitive and high-affinity methods to measure gene expression inside living cells have proven to be invaluable in regards to understanding fundamental processes such as cell differentiation, reprogramming, regeneration and cancer genesis. One tool for transcription visualization on single...

  1. Fusing Bluetooth Beacon Data with Wi-Fi Radiomaps for Improved Indoor Localization.

    Science.gov (United States)

    Kanaris, Loizos; Kokkinis, Akis; Liotta, Antonio; Stavrou, Stavros

    2017-04-10

    Indoor user localization and tracking are instrumental to a broad range of services and applications in the Internet of Things (IoT) and particularly in Body Sensor Networks (BSN) and Ambient Assisted Living (AAL) scenarios. Due to the widespread availability of IEEE 802.11, many localization platforms have been proposed, based on the Wi-Fi Received Signal Strength (RSS) indicator, using algorithms such as K -Nearest Neighbour (KNN), Maximum A Posteriori (MAP) and Minimum Mean Square Error (MMSE). In this paper, we introduce a hybrid method that combines the simplicity (and low cost) of Bluetooth Low Energy (BLE) and the popular 802.11 infrastructure, to improve the accuracy of indoor localization platforms. Building on KNN, we propose a new positioning algorithm (dubbed i-KNN) which is able to filter the initial fingerprint dataset (i.e., the radiomap), after considering the proximity of RSS fingerprints with respect to the BLE devices. In this way, i-KNN provides an optimised small subset of possible user locations, based on which it finally estimates the user position. The proposed methodology achieves fast positioning estimation due to the utilization of a fragment of the initial fingerprint dataset, while at the same time improves positioning accuracy by minimizing any calculation errors.

  2. Fusing Bluetooth Beacon Data with Wi-Fi Radiomaps for Improved Indoor Localization

    Directory of Open Access Journals (Sweden)

    Loizos Kanaris

    2017-04-01

    Full Text Available Indoor user localization and tracking are instrumental to a broad range of services and applications in the Internet of Things (IoT and particularly in Body Sensor Networks (BSN and Ambient Assisted Living (AAL scenarios. Due to the widespread availability of IEEE 802.11, many localization platforms have been proposed, based on the Wi-Fi Received Signal Strength (RSS indicator, using algorithms such as K-Nearest Neighbour (KNN, Maximum A Posteriori (MAP and Minimum Mean Square Error (MMSE. In this paper, we introduce a hybrid method that combines the simplicity (and low cost of Bluetooth Low Energy (BLE and the popular 802.11 infrastructure, to improve the accuracy of indoor localization platforms. Building on KNN, we propose a new positioning algorithm (dubbed i-KNN which is able to filter the initial fingerprint dataset (i.e., the radiomap, after considering the proximity of RSS fingerprints with respect to the BLE devices. In this way, i-KNN provides an optimised small subset of possible user locations, based on which it finally estimates the user position. The proposed methodology achieves fast positioning estimation due to the utilization of a fragment of the initial fingerprint dataset, while at the same time improves positioning accuracy by minimizing any calculation errors.

  3. An Indoor Location-Based Control System Using Bluetooth Beacons for IoT Systems

    Directory of Open Access Journals (Sweden)

    Jun-Ho Huh

    2017-12-01

    Full Text Available The indoor location-based control system estimates the indoor position of a user to provide the service he/she requires. The major elements involved in the system are the localization server, service-provision client, user application positioning technology. The localization server controls access of terminal devices (e.g., Smart Phones and other wireless devices to determine their locations within a specified space first and then the service-provision client initiates required services such as indoor navigation and monitoring/surveillance. The user application provides necessary data to let the server to localize the devices or allow the user to receive various services from the client. The major technological elements involved in this system are indoor space partition method, Bluetooth 4.0, RSSI (Received Signal Strength Indication and trilateration. The system also employs the BLE communication technology when determining the position of the user in an indoor space. The position information obtained is then used to control a specific device(s. These technologies are fundamental in achieving a “Smart Living”. An indoor location-based control system that provides services by estimating user’s indoor locations has been implemented in this study (First scenario. The algorithm introduced in this study (Second scenario is effective in extracting valid samples from the RSSI dataset but has it has some drawbacks as well. Although we used a range-average algorithm that measures the shortest distance, there are some limitations because the measurement results depend on the sample size and the sample efficiency depends on sampling speeds and environmental changes. However, the Bluetooth system can be implemented at a relatively low cost so that once the problem of precision is solved, it can be applied to various fields.

  4. Prototyping and testing of a fully autonomous road construction beacon, the iCone.

    Science.gov (United States)

    2010-04-01

    A revolutionary portable traffic monitoring device is developed, extensively prototyped and thoroughly tested throughout the State of New York as well as several other states. The resulting device, trademarked as the iCone, simplifies the process o...

  5. Round-the-clock performance of coronary CT angiography for suspected acute coronary syndrome: Results from the BEACON trial

    NARCIS (Netherlands)

    M.M. Lubbers (Marisa); A. Dedic (Admir); A. Kurata (Akira); M.L. Dijkshoorn (Marcel); J. Schaap (Jeroen); Lammers, J. (Jeroen); E.J. Lamfers (Evert); B.J.W.M. Rensing (Benno); R.L. Braam (Richard L.); H.M. Nathoe (Hendrik); J.C. Post; P.P.M. Rood (Pleunie); C.J. Schultz (Carl); A. Moelker (Adriaan); M. Ouhlous (Mohamed); B.M. van Dalen (Bas); H. Boersma (Eric); K. Nieman (Koen)

    2017-01-01

    textabstractObjective: To assess the image quality of coronary CT angiography (CCTA) for suspected acute coronary syndrome (ACS) outside office hours. Methods: Patients with symptoms suggestive of an ACS underwent CCTA at the emergency department 24 hours, 7 days a week. A total of 118 patients, of

  6. Measuring Tilt and Focus for Sodium Beacon Adaptive Optics on the Starfile 3.5 Meter Telescope -- Conference Proceedings (Preprint)

    Science.gov (United States)

    2008-09-01

    in detail by Link and Foucault . [2] They show these approaches to focus control have comparable performance in the presence of atmospheric turbulence... Foucault B., Investigation of focus control for NGAS, Starfire Optical Range internal memo, 8 May 2007. 3. Goodman J., Introduction to Fourier Optics

  7. Diseño e implementación de un sistema de alarma basado en beacons Bluetooth

    OpenAIRE

    Fernández Cofrades, Carlos

    2017-01-01

    Un problema generalizado en los sistemas de alarmas actuales es la intrusión de ladrones en garajes comunitarios aprovechando la entrada de algún vecino: al entrar el vecino en el garaje, el ladrón se esconde tras de él y aprovecha el tiempo que tarda en cerrarse la puerta para entrar al garaje sin ser detectado. De este modo, cuando el vecino se haya ido, el ladrón podrá moverse por el garaje y realizar el robo. Para salir del garaje, el intruso podrá usar la misma técnica que usó al entrar....

  8. iBILL: Using iBeacon and Inertial Sensors for Accurate Indoor Localization in Large Open Areas

    OpenAIRE

    Wu, Xudong; Shen, Ruofei; Fu, Luoyi; Tian, Xiaohua; Liu, Peng; Wang, Xinbing

    2017-01-01

    As a key technology that is widely adopted in location-based services (LBS), indoor localization has received considerable attention in both research and industrial areas. Despite the huge efforts made for localization using smartphone inertial sensors, its performance is still unsatisfactory in large open areas, such as halls, supermarkets, and museums, due to accumulated errors arising from the uncertainty of users’ mobility and fluctuations of magnetic field. Regarding that, this paper pre...

  9. Resource and Technology: A beacon for change in the reform of Jamaica's secondary education system - or a "pipedream"?

    Science.gov (United States)

    Jennings, Zellynne

    2012-04-01

    Different strategies are being employed worldwide to prepare school-leavers for the world of work. Central to the Reform of Secondary Education (ROSE) in Jamaica in the 1990s was the achievement of goals of access, equity and quality through the implementation of a common curriculum in all schools. Within this reform, Resource and Technology (R&T) was an innovation designed to develop the creative potential in technology and to transform pedagogical practices from being teacher-centred to being student-centred. This paper examines how teachers and principals involved in the implementation of R&T perceive its attributes, such as need and relevance and observability. The findings reveal how the achievement of goals was frustrated and which challenges the users faced, including a lack of clarity of the means for implementing R&T.

  10. Molecular detection of Phytophthora ramorum by real-time PCR using Taqman, SYBR Green and molecular beacons with three genes

    Science.gov (United States)

    G.J. Bilodeau; C.A. Lévesque; A.W.A.M. De Cock; C. Duchaine; G. Kristjansson; R.C. Hamelin

    2006-01-01

    Sudden oak death, caused by Phytophthora ramorum, is a severe disease that can affect numerous species of trees and shrubs. This pathogen has been spread via nursery stock, and quarantine measures are currently in place to prevent further spread. Molecular assays have been developed to rapidly detect and identify P. ramorum, but...

  11. Observability analysis of 2D single-beacon navigation in the presence of constant currents for two classes of maneuvers

    Digital Repository Service at National Institute of Oceanography (India)

    Crasta, N.; Bayat, M.; Aguiar, A.P.; Pascoal, A.M.

    . Further, the observability issue has been addressed using different ap- proaches such as linearization, (Gadre and Stilwell, 2004)- (Gadre and Stilwell, 2005), geometric methods (Arrichiello et al., 2011) and algebraic methods (Jouffroy and Reger, 2006... observability analysis is carried out in the algebraic set-up. In other words, the state is expressed by a function whose argu- ments are the output of the system and its first derivative in polar coordinates. In (Parlangeli et al., 2012), the observability...

  12. A novel strategy for human papillomavirus detection and genotyping with SybrGreen and molecular beacon polymerase chain reaction

    NARCIS (Netherlands)

    Szuhai, K; Sandhaus, E; Kolkman-Uljee, SM; Lemaitre, M; Truffert, JC; Dirks, RW; Tanke, HJ; Fleuren, GJ; Schuuring, E; Raap, AK

    2001-01-01

    Human papillomaviruses (HPVs) play an important role in the pathogenesis of cervical cancer. For identification of the large number of different HPV types found in (pre)malignant lesions, a robust methodology is needed that combines general HPV detection with HPV genotyping. We have developed for

  13. HIA 2016 DOE Zero Energy Ready Home Case Study: United Way of Long Island, United Veterans, Beacon House, Deer Park, NY

    Energy Technology Data Exchange (ETDEWEB)

    Pacific Northwest National Laboratory

    2016-09-01

    Case study of a DOE 2016 Housing Innovation Award winning affordable home in the mixed-humid climate that met the DOE Zero Energy Ready Home criteria and achieved a HERS 32 without PV or HERS 9 with PV.

  14. 'Being the bridge and the beacon': a qualitative study of the characteristics and functions of the liaison role in child and family health services in Australia.

    Science.gov (United States)

    Olley, Hannah; Psaila, Kim; Fowler, Cathrine; Kruske, Sue; Homer, Caroline; Schmied, Virginia

    2017-01-01

    This article explores the characteristics and functions of the liaison role in child and family health services in Australia. Liaison roles are increasingly being used to improve communication between health services and professionals and to facilitate access to support for individuals and families in need. Nurses are commonly, although not always, the professionals who undertake these roles. Research on the role and outcomes of liaison positions in child and family health services is limited in Australia and internationally. A qualitative interpretive design informed this study. Interviews and focus groups were conducted with 40 liaison and other health professionals, primarily nurses, working with families with newborn and young children in two Australian States. Data were analysed thematically. Three major themes were identified reflecting the importance of defining the role and tasks which included building bridges between services and professionals, supporting families during transition between services and supporting clinicians. Several facilitators and barriers were identified, including concerns about sustainability of the roles. Professionals working in a liaison role in child and family health services emphasise that these positions have the potential to link services and professionals, thereby providing more effective care pathways for children and families especially for those with complex and multiple vulnerabilities. While a few children and family health services in Australia provide liaison services, the extent of liaison support and the outcomes for families in Australia is unknown. Nurses working with children and families are the most likely health professionals to undertake a liaison role. In many nursing contexts, liaison roles are relatively new and those in the role have the responsibility to define the key purpose of their role. Liaison roles are multifaceted requiring the nurse to have excellent communication and negotiation skills to effectively link diverse professionals and services, while simultaneously engaging with and supporting vulnerable families and children. Nurses in these roles also support and educate clinical colleagues. © 2016 John Wiley & Sons Ltd.

  15. Molecular Beacon Enables Combination of Highly Processive and Highly Sensitive Rolling Circle Amplification Readouts for Detection of DNA-Modifying Enzymes

    DEFF Research Database (Denmark)

    Kristoffersen, Emil Laust; Gonzales, Maria; Stougaard, Magnus

    2015-01-01

    Here we present an optimized readout format for detection of the circularized products from a DNA-based sensor for measurement of DNA-modifying enzymes including DNA Topoisomerase I. The basic design of the DNA-sensor relies on the use of a substrate that can be circularized by the activity of DN...

  16. Near-Infrared Fluorescence Imaging Guided Therapy: Molecular Beacon-Based Photosensitizers Triggered by Breast Cancer-Specific mRNA

    Science.gov (United States)

    2007-05-01

    C.; Stephens, P.; Edkins, S.; Clegg, S.; Teague, J.; Woffendin, H.; Garnett, M. J.; Bottomley, W.; Davis, N.; Dicks, E.; Ewing, R.; Floyd , Y...Weissleder, R. Radiology, 1999, 213, 866-70. [98] Chen, J.; Tung, C. H.; Allport , J. R.; Chen, S.; Weissleder, R.; Huang, P. L. Circulation, 2005, 111

  17. National Dam Safety Program. Mt. Beacon Reservoir (Inventory Number NY- 26), Hudson River Basin, Dutchess County, New York. Phase I Inspection Report

    Science.gov (United States)

    1980-09-30

    7~~-£ ,AJI ~1 U / I, I ,~/ I ’V ) I -..-.- S S - __ ’~ ’~A .A~~~-L-’-.-- - - .q 4ky 44 x 1 ~ij ’ 2? /~’e 0/u/cc/ -~ - ~~s1 I 7~1>,cc/ 5~c//~𔄁 Jcz

  18. Coronary plaque structural stress is associated with plaque composition and subtype and higher in acute coronary syndrome: the BEACON I (Biomechanical Evaluation of Atheromatous Coronary Arteries) study.

    Science.gov (United States)

    Teng, Zhongzhao; Brown, Adam J; Calvert, Patrick A; Parker, Richard A; Obaid, Daniel R; Huang, Yuan; Hoole, Stephen P; West, Nick E J; Gillard, Jonathan H; Bennett, Martin R

    2014-05-01

    Atherosclerotic plaques underlying most myocardial infarctions have thin fibrous caps and large necrotic cores; however, these features alone do not reliably identify plaques that rupture. Rupture occurs when plaque structural stress (PSS) exceeds mechanical strength. We examined whether PSS could be calculated in vivo based on virtual histology (VH) intravascular ultrasound and whether PSS varied according to plaque composition, subtype, or clinical presentation. A total of 4429 VH intravascular ultrasound frames from 53 patients were analyzed, identifying 99 584 individual plaque components. PSS was calculated by finite element analysis in whole vessels, in individual plaques, and in higher-risk regions (plaque burden ≥70%, mean luminal area ≤4 mm(2), noncalcified VH-defined thin-cap fibroatheroma). Plaque components including total area/arc of calcification (R(2)=0.33; PPSS. PSS was higher in noncalcified VH-defined thin-cap fibroatheroma compared with thick-cap fibroatheromas (median [Q1-Q3], 8.44 [6.97-10.64] versus 7.63 [6.37-9.68]; P=0.002). PSS was also higher in patients with an acute coronary syndrome, where mean luminal area ≤4 mm(2) (8.24 [7.06-9.93] versus 7.72 [6.33-9.34]; P=0.03), plaque burden ≥70% (9.18 [7.44-10.88] versus 7.93 [6.16-9.46]; P=0.02), and in noncalcified VH-defined thin-cap fibroatheroma (9.23 [7.33-11.44] versus 7.65 [6.45-8.62]; P=0.02). Finally, PSS increased the positive predictive value for VH intravascular ultrasound to identify clinical presentation. Finite element analysis modeling demonstrates that structural stress is highly variable within plaques, with increased PSS associated with plaque composition, subtype, and higher-risk regions in patients with acute coronary syndrome. PSS may represent a novel tool to analyze the dynamic behavior of coronary plaques with the potential to improve prediction of plaque rupture. © 2014 American Heart Association, Inc.

  19. Polymorphisms in Genes of Relevance for Oestrogen and Oxytocin Pathways and Risk of Barrett's Oesophagus and Oesophageal Adenocarcinoma: A Pooled Analysis from the BEACON Consortium.

    Science.gov (United States)

    Lagergren, Katarina; Ek, Weronica E; Levine, David; Chow, Wong-Ho; Bernstein, Leslie; Casson, Alan G; Risch, Harvey A; Shaheen, Nicholas J; Bird, Nigel C; Reid, Brian J; Corley, Douglas A; Hardie, Laura J; Wu, Anna H; Fitzgerald, Rebecca C; Pharoah, Paul; Caldas, Carlos; Romero, Yvonne; Vaughan, Thomas L; MacGregor, Stuart; Whiteman, David; Westberg, Lars; Nyren, Olof; Lagergren, Jesper

    2015-01-01

    The strong male predominance in oesophageal adenocarcinoma (OAC) and Barrett's oesophagus (BO) continues to puzzle. Hormonal influence, e.g. oestrogen or oxytocin, might contribute. This genetic-epidemiological study pooled 14 studies from three continents, Australia, Europe, and North America. Polymorphisms in 3 key genes coding for the oestrogen pathway (receptor alpha (ESR1), receptor beta (ESR2), and aromatase (CYP19A1)), and 3 key genes of the oxytocin pathway (the oxytocin receptor (OXTR), oxytocin protein (OXT), and cyclic ADP ribose hydrolase glycoprotein (CD38)), were analysed using a gene-based approach, versatile gene-based test association study (VEGAS). Among 1508 OAC patients, 2383 BO patients, and 2170 controls, genetic variants within ESR1 were associated with BO in males (p = 0.0058) and an increased risk of OAC and BO combined in males (p = 0.0023). Genetic variants within OXTR were associated with an increased risk of BO in both sexes combined (p = 0.0035) and in males (p = 0.0012). We followed up these suggestive findings in a further smaller data set, but found no replication. There were no significant associations between the other 4 genes studied and risk of OAC, BO, separately on in combination, in males and females combined or in males only. Genetic variants in the oestrogen receptor alpha and the oxytocin receptor may be associated with an increased risk of BO or OAC, but replication in other large samples are needed.

  20. Polymorphisms in Genes of Relevance for Oestrogen and Oxytocin Pathways and Risk of Barrett's Oesophagus and Oesophageal Adenocarcinoma: A Pooled Analysis from the BEACON Consortium.

    Directory of Open Access Journals (Sweden)

    Katarina Lagergren

    Full Text Available The strong male predominance in oesophageal adenocarcinoma (OAC and Barrett's oesophagus (BO continues to puzzle. Hormonal influence, e.g. oestrogen or oxytocin, might contribute.This genetic-epidemiological study pooled 14 studies from three continents, Australia, Europe, and North America. Polymorphisms in 3 key genes coding for the oestrogen pathway (receptor alpha (ESR1, receptor beta (ESR2, and aromatase (CYP19A1, and 3 key genes of the oxytocin pathway (the oxytocin receptor (OXTR, oxytocin protein (OXT, and cyclic ADP ribose hydrolase glycoprotein (CD38, were analysed using a gene-based approach, versatile gene-based test association study (VEGAS.Among 1508 OAC patients, 2383 BO patients, and 2170 controls, genetic variants within ESR1 were associated with BO in males (p = 0.0058 and an increased risk of OAC and BO combined in males (p = 0.0023. Genetic variants within OXTR were associated with an increased risk of BO in both sexes combined (p = 0.0035 and in males (p = 0.0012. We followed up these suggestive findings in a further smaller data set, but found no replication. There were no significant associations between the other 4 genes studied and risk of OAC, BO, separately on in combination, in males and females combined or in males only.Genetic variants in the oestrogen receptor alpha and the oxytocin receptor may be associated with an increased risk of BO or OAC, but replication in other large samples are needed.

  1. Lessons Learnt from the Optical Communications Demonstrator (OCD)

    Science.gov (United States)

    Jeganathan, M.; Portillo, A.; Racho, C.; Lee, S.; Erickson, D.; DePew, J.; Monacos, S.; Biswas, A.

    1999-01-01

    The Optical Communications Demonstrator (OCD) is a laboratory based lasercom terminal that was developed to validate several key technologies such as precision beam pointing, high bandwidth beacon tracking and beacon acquisition.

  2. Polarization tracking system for free-space optical communication, including quantum communication

    Science.gov (United States)

    Nordholt, Jane Elizabeth; Newell, Raymond Thorson; Peterson, Charles Glen; Hughes, Richard John

    2018-01-09

    Quantum communication transmitters include beacon lasers that transmit a beacon optical signal in a predetermined state of polarization such as one of the states of polarization of a quantum communication basis. Changes in the beacon polarization are detected at a receiver, and a retarder is adjusted so that the states of polarization in a received quantum communication optical signal are matched to basis polarizations. The beacon and QC signals can be at different wavelengths so that the beacon does not interfere with detection and decoding of the QC optical signal.

  3. 75 FR 42014 - Proposed Amendment of Class E Airspace; San Clemente, CA

    Science.gov (United States)

    2010-07-20

    ... Island Non- Directional Radio Beacon (NDB) at San Clemente Island NALF (Frederick Sherman Field) has made...: Eldon Taylor, Federal Aviation Administration, Operations Support Group, Western Service Center, 1601...

  4. Evaluation of electronic health record implementation on pharmacist interventions related to oral chemotherapy management.

    Science.gov (United States)

    Finn, Anastasia; Bondarenka, Carolyn; Edwards, Kathy; Hartwell, Rebekah; Letton, Cathy; Perez, Andy

    2017-12-01

    Background With the ever growing arsenal of oral chemotherapy agents now available, cancer treatment is being increasingly managed in the outpatient setting. However, oral chemotherapy use is often associated with several potential obstacles and complications. In order to provide optimal patient safety and oral chemotherapy monitoring, our institution implemented an oral chemotherapy program managed by clinical pharmacists electronically through Epic Beacon. Objective To describe implementation of a novel pharmacist-managed oral chemotherapy program and evaluate pharmacist interventions before and after implementation of an oral chemotherapy program. Methods This was a single-center retrospective chart review of documented pharmacy interventions for oral chemotherapy prescriptions during three months prior to as well as three months following Epic Beacon implementation. Time periods for data inclusion were October-December 2013 (pre-Beacon) and October-December 2014 (post-Beacon). Patients included in the study had one or more oral chemotherapy orders during the pre-Beacon period, the post-Beacon period, or both pre- and post-Beacon. Our analysis did not include oral chemotherapy orders that were placed outside of a treatment plan in the post-Beacon period. Results A total of 240 patients with 450 total oral chemotherapy orders were assessed over the duration of the study. Beacon implementation allowed a greater number of oral chemotherapy orders to be reviewed, with 134 oral chemotherapy orders reviewed in the study period prior to Beacon implementation and 316 orders reviewed in the post-Beacon period. Additionally, there were 660% more pharmacist interventions (89 interventions pre-Beacon versus 681 interventions post-Beacon), with an increased focus on coordination of care, chemotherapy calendar coordination, and assistance with treatment plans. Furthermore, implementation of Epic Beacon allowed identification of over 500% more chemotherapy order errors (41

  5. Altruistic Backoff: Collision Avoidance for Receiver-Initiated MAC Protocols for Wireless Sensor Networks

    DEFF Research Database (Denmark)

    Fafoutis, Xenofon; Orfanidis, Charalampos; Dragoni, Nicola

    2014-01-01

    points where collisions are likely to happen. In this paper, we present altruistic backoff (AB), a novel collision avoidance mechanism that aims to avoid collisions before the transmission of a beacon. As a result of an early backoff, senders spend less time in idle listening waiting for a beacon, thus...

  6. Method of identifying hairpin DNA probes by partial fold analysis

    Science.gov (United States)

    Miller, Benjamin L [Penfield, NY; Strohsahl, Christopher M [Saugerties, NY

    2009-10-06

    Method of identifying molecular beacons in which a secondary structure prediction algorithm is employed to identify oligonucleotide sequences within a target gene having the requisite hairpin structure. Isolated oligonucleotides, molecular beacons prepared from those oligonucleotides, and their use are also disclosed.

  7. Hypermedia Applications for Army Installation Master Planning

    Science.gov (United States)

    1993-01-01

    node E, "pizza 8 1. Habermas . Communication and the Evolution of Society (Beacon Press, 1979). 9 J. Forester. 10 1. Friend and A. Hickling, Planning...Planning Under Pressure (Pergamon, 1987). Gillenson. M.L., Database (Wiley, 1985). Habermas , J., Communication and the Evolution of Society (Beacon Press

  8. Method for Improving Indoor Positioning Accuracy Using Extended Kalman Filter

    Directory of Open Access Journals (Sweden)

    Seoung-Hyeon Lee

    2016-01-01

    Full Text Available Beacons using bluetooth low-energy (BLE technology have emerged as a new paradigm of indoor positioning service (IPS because of their advantages such as low power consumption, miniaturization, wide signal range, and low cost. However, the beacon performance is poor in terms of the indoor positioning accuracy because of noise, motion, and fading, all of which are characteristics of a bluetooth signal and depend on the installation location. Therefore, it is necessary to improve the accuracy of beacon-based indoor positioning technology by fusing it with existing indoor positioning technology, which uses Wi-Fi, ZigBee, and so forth. This study proposes a beacon-based indoor positioning method using an extended Kalman filter that recursively processes input data including noise. After defining the movement of a smartphone on a flat two-dimensional surface, it was assumed that the beacon signal is nonlinear. Then, the standard deviation and properties of the beacon signal were analyzed. According to the analysis results, an extended Kalman filter was designed and the accuracy of the smartphone’s indoor position was analyzed through simulations and tests. The proposed technique achieved good indoor positioning accuracy, with errors of 0.26 m and 0.28 m from the average x- and y-coordinates, respectively, based solely on the beacon signal.

  9. Spatial Audio Displays for Improving Safety and Enhancing Situation Awareness in General Aviation Environments

    National Research Council Canada - National Science Library

    Simpson, Brian D; Brungart, Douglas S; Gilkey, Robert H; McKinley, Richard L

    2005-01-01

    ... and an attitude indicator. Performance was measured in tasks that required pilots to fly in the direction of a spatial audio navigation beacon, and use an auditory artificial horizon display to detect changes in attitude...

  10. Santa Barbara Littoral Cell CRSMP Beaches 2009

    Data.gov (United States)

    California Department of Resources — Several criteria were used for beach selection. BEACON 's Coastal Regional Sediment Management Plan included all of the most popular beaches in the two counties...

  11. 36 CFR 14.78 - Applications.

    Science.gov (United States)

    2010-07-01

    ... given (such as airway beacon, coal mine, and irrigation pumps). (3) The application and maps shall... issued in excess of such sizes in the absence of a satisfactory showing of the need therefor. (6)(i) A...

  12. 78 FR 77587 - Waiver for Marking Sunken Vessels With a Light at Night

    Science.gov (United States)

    2013-12-24

    ...). Executive Order 13563 emphasizes the importance of quantifying both costs and benefits, of reducing costs... marking established, including color and shape of buoy or other beacon and characteristic of the light, if...

  13. Depths to Ice-cemented Soils in High-elevation Quartermain Mountains, Dry Valleys, Antarctica, Version 1

    Data.gov (United States)

    National Aeronautics and Space Administration — This data set is comprised of four surveyed valleys focusing on the depth to ground ice in the high-elevation Quartermain Mountains in the Beacon Valley area:...

  14. Distributed magnetic field positioning system using code division multiple access

    Science.gov (United States)

    Prigge, Eric A. (Inventor)

    2003-01-01

    An apparatus and methods for a magnetic field positioning system use a fundamentally different, and advantageous, signal structure and multiple access method, known as Code Division Multiple Access (CDMA). This signal architecture, when combined with processing methods, leads to advantages over the existing technologies, especially when applied to a system with a large number of magnetic field generators (beacons). Beacons at known positions generate coded magnetic fields, and a magnetic sensor measures a sum field and decomposes it into component fields to determine the sensor position and orientation. The apparatus and methods can have a large `building-sized` coverage area. The system allows for numerous beacons to be distributed throughout an area at a number of different locations. A method to estimate position and attitude, with no prior knowledge, uses dipole fields produced by these beacons in different locations.

  15. Station Explorer for X-Ray Timing and Navigation Technology (SEXTANT)

    Data.gov (United States)

    National Aeronautics and Space Administration — Use millisecond period X-ray emitting neutron stars (Millisecond Pulsars) as beacons to enable GPS-like autonomous navigation anywhere in Solar System and beyond....

  16. Santa Barbara Littoral Cell CRSMP Sensitive Habitat Sites 2009

    Data.gov (United States)

    California Natural Resource Agency — Ecological areas as identified and collected by BEACON and submitted to CSMW as part of the Central Coast (from Pt. Conception to Pt. Mugu) Coastal Regional Sediment...

  17. Error Estimation for the Linearized Auto-Localization Algorithm

    Directory of Open Access Journals (Sweden)

    Fernando Seco

    2012-02-01

    Full Text Available The Linearized Auto-Localization (LAL algorithm estimates the position of beacon nodes in Local Positioning Systems (LPSs, using only the distance measurements to a mobile node whose position is also unknown. The LAL algorithm calculates the inter-beacon distances, used for the estimation of the beacons’ positions, from the linearized trilateration equations. In this paper we propose a method to estimate the propagation of the errors of the inter-beacon distances obtained with the LAL algorithm, based on a first order Taylor approximation of the equations. Since the method depends on such approximation, a confidence parameter τ is defined to measure the reliability of the estimated error. Field evaluations showed that by applying this information to an improved weighted-based auto-localization algorithm (WLAL, the standard deviation of the inter-beacon distances can be improved by more than 30% on average with respect to the original LAL method.

  18. Santa Barbara Littoral Cell CRSMP Beaches 2009

    Data.gov (United States)

    California Natural Resource Agency — Several criteria were used for beach selection. BEACON 's Coastal Regional Sediment Management Plan included all of the most popular beaches in the two counties...

  19. 33 CFR 334.1190 - Hood Canal and Dabob Bay, Wash.; naval non-explosive torpedo testing area.

    Science.gov (United States)

    2010-07-01

    ... craft shall not work their screws or otherwise generate other than incidental noise in the area during periods of actual testing, which will be indicated by flashing red beacons at strategic locations, and all...

  20. Mobile means for the monitoring of atmospheric contamination in a reactor building

    International Nuclear Information System (INIS)

    Marques, S.; Lestang, M.

    2009-01-01

    After having evoked the context and challenges of contamination monitoring when exploiting nuclear reactors, the authors discuss the representativeness of the atmospheric contamination measurement as it depends on the different physicochemical forms of radionuclides present in the circuits. They indicate the different gaseous or aerosol radioactive elements which are monitored within EDF installations. They discuss the incorporation of monitoring means at the installation design level, briefly present the use of beacons inside and outside the reactor building. They describe how monitoring is organized on the basis of alert threshold adjustments: an investigation threshold and an evacuation threshold. They discuss the beacon (or sensor) selection and indicate recommendations for their implementation for optimization purposes. They indicate where these beacons are installed and evoke the experimentation of networked mobile beacons with data remote transmission

  1. Mobile means for the monitoring of atmospheric contamination in a reactor building; Moyens mobiles de surveillance de la contamination atmospherique en BR

    Energy Technology Data Exchange (ETDEWEB)

    Marques, S.; Lestang, M. [EDF/DPN/GPRE/IRP, 93 - Saint Denis (France)

    2009-07-01

    After having evoked the context and challenges of contamination monitoring when exploiting nuclear reactors, the authors discuss the representativeness of the atmospheric contamination measurement as it depends on the different physicochemical forms of radionuclides present in the circuits. They indicate the different gaseous or aerosol radioactive elements which are monitored within EDF installations. They discuss the incorporation of monitoring means at the installation design level, briefly present the use of beacons inside and outside the reactor building. They describe how monitoring is organized on the basis of alert threshold adjustments: an investigation threshold and an evacuation threshold. They discuss the beacon (or sensor) selection and indicate recommendations for their implementation for optimization purposes. They indicate where these beacons are installed and evoke the experimentation of networked mobile beacons with data remote transmission

  2. Santa Barbara Littoral Cell CRSMP Sensitive Habitat Areas 2009

    Data.gov (United States)

    California Department of Resources — Ecological areas as identified and collected by BEACON and submitted to CSMW as part of the Central Coast (from Pt. Conception to Pt. Mugu) Coastal Regional Sediment...

  3. Santa Barbara Littoral Cell CRSMP Sensitive Habitat Sites 2009

    Data.gov (United States)

    California Department of Resources — Ecological areas as identified and collected by BEACON and submitted to CSMW as part of the Central Coast (from Pt. Conception to Pt. Mugu) Coastal Regional Sediment...

  4. RFID-Enabled Navigation and Communication Networks for Long-Duration Space Missions, Phase I

    Data.gov (United States)

    National Aeronautics and Space Administration — Virtual EM Inc. proposes a system that employs semi-passive RFID sensors with carbon nanotube inkjet-printed antenna and solar powered mesh-networked beacons. The...

  5. Data Link Test and Analysis System/ATCRBS Transponder Test System Technical Reference

    Science.gov (United States)

    1990-05-01

    This document references material for personnel using or making software changes : to the Data Link Test and Analysis System (DATAS) for Air Traffic Control Radar : Beacon System (ATCRBS) transponder testing and data collection. This is one of : a se...

  6. The Radar Correlation and Interpolation (C&I) Algorithms Deployed in the ASR-9 Processor Augmentation Card (9PAC)

    National Research Council Canada - National Science Library

    Elkin, G

    2001-01-01

    .... The increased processing speed and memory size of the 9PAC hardware made it possible for new surveillance algorithms to be developed in software in order to provide improved primary radar and beacon...

  7. Cities spearhead climate action

    Science.gov (United States)

    Watts, Mark

    2017-08-01

    Following President Trump's withdrawal from the Paris Agreement, cities worldwide have pledged support to combat climate change. Along with a growing coalition of businesses and institutions, cities represent a beacon of hope for carbon reduction in politically tumultuous times.

  8. 40 CFR 262.10 - Purpose, scope, and applicability.

    Science.gov (United States)

    2010-07-01

    ..., Biology, Psychology, Anthropology, Geology and Earth Sciences, and Environmental, Coastal and Ocean....10 Section 262.10 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES..., Geology, Physics, Psychology Merkert Chemistry Building, 2609 Beacon St., Boston, MA, Higgins Building...

  9. Energy-efficient buildings and apartments. Investors, buyers and tenants demand sustainability; Effizienz-Immobilien. Investoren, Anleger und Mieter verlangen Nachhaltigkeit

    Energy Technology Data Exchange (ETDEWEB)

    Balck, Henning [Balck und Partner, Heidelberg (Germany)

    2009-11-15

    Sustainability has become the talk of the town and a beacon of hope for pioneering big enterprises, medium-sized businesses, and investment companies who are designing and developing their products with the goal of sustainability in mind. (orig.)

  10. 77 FR 13350 - Certain Automotive GPS Navigation Systems, Components Thereof, and Products Containing Same...

    Science.gov (United States)

    2012-03-06

    ...-2000. General information concerning the Commission may also be obtained by accessing its Internet... Company of Detroit, Michigan (``GM''). On December 2, 2011, Beacon filed a motion for leave to amend the...

  11. The young and the digital, by S. Craig Watkins [book review

    OpenAIRE

    Melanie E. S. Kohnen

    2011-01-01

    Review of S. Craig Watkins, The young and the digital: What migration to social-networking sites, games, and anytime, anywhere media means for our future. Boston: Beacon Press, 2009, paperback, $18 (272p) ISBN 978-0807006160.

  12. The Indoor Localization and Tracking Estimation Method of Mobile Targets in Three-Dimensional Wireless Sensor Networks

    Directory of Open Access Journals (Sweden)

    Zixi Jia

    2015-11-01

    Full Text Available Indoor localization is a significant research area in wireless sensor networks (WSNs. Generally, the nodes of WSNs are deployed in the same plane, i.e., the floor, as the target to be positioned, which causes the sensing signal to be influenced or even blocked by unpredictable obstacles, like furniture. However, a 3D system, like Cricket, can reduce the negative impact of obstacles to the maximum extent and guarantee the sensing signal transmission by using the line of sight (LOS. However, most of the traditional localization methods are not available for the new deployment mode. In this paper, we propose the self-localization of beacons method based on the Cayley–Menger determinant, which can determine the positions of beacons stuck in the ceiling; and differential sensitivity analysis (DSA is also applied to eliminate measurement errors in measurement data fusion. Then, the calibration of beacons scheme is proposed to further refine the locations of beacons by the mobile robot. According to the robot’s motion model based on dead reckoning, which is the process of determining one’s current position, we employ the H ∞ filter and the strong tracking filter (STF to calibrate the rough locations, respectively. Lastly, the optimal node selection scheme based on geometric dilution precision (GDOP is presented here, which is able to pick the group of beacons with the minimum GDOP from all of the beacons. Then, we propose the GDOP-based weighting estimation method (GWEM to associate redundant information with the position of the target. To verify the proposed methods in the paper, we design and conduct a simulation and an experiment in an indoor setting. Compared to EKF and the H ∞ filter, the adopted STF method can more effectively calibrate the locations of beacons; GWEM can provide centimeter-level precision in 3D environments by using the combination of beacons that minimizes GDOP.

  13. Multi-Channel Cluster Tree for 802.15.4 Wireless Sensor Networks

    OpenAIRE

    Abdeddaim, Nazim; Theoleyre, Fabrice; Rousseau, Franck; Duda, Andrzej

    2012-01-01

    Selective A - Session: MAC12: MAC for mesh, ad hoc, relay, and sensor networks II; International audience; We propose MCCT (Multi-Channel Cluster Tree), a cluster-tree construction protocol for nodes in IEEE 802.15.4 beacon-enabled mode. By multiplexing transmissions across orthogonal channels, we reduce collisions between control and data frames, which leads to better packet delivery rate and fairness. We propose a method for constructing a cluster-tree suitable for minimizing beacon collisi...

  14. Design & development fo a 20-MW flywheel-based frequency regulation power plant : a study for the DOE Energy Storage Systems program.

    Energy Technology Data Exchange (ETDEWEB)

    Rounds, Robert (Beacon Power, Tyngsboro, MA); Peek, Georgianne Huff

    2009-01-01

    This report describes the successful efforts of Beacon Power to design and develop a 20-MW frequency regulation power plant based solely on flywheels. Beacon's Smart Matrix (Flywheel) Systems regulation power plant, unlike coal or natural gas generators, will not burn fossil fuel or directly produce particulates or other air emissions and will have the ability to ramp up or down in a matter of seconds. The report describes how data from the scaled Beacon system, deployed in California and New York, proved that the flywheel-based systems provided faster responding regulation services in terms of cost-performance and environmental impact. Included in the report is a description of Beacon's design package for a generic, multi-MW flywheel-based regulation power plant that allows accurate bids from a design/build contractor and Beacon's recommendations for site requirements that would ensure the fastest possible construction. The paper concludes with a statement about Beacon's plans for a lower cost, modular-style substation based on the 20-MW design.

  15. Применение маячков Beacon и технологии Bluetooth Low Energy для построения систем навигации в зданиях

    OpenAIRE

    Фальков, Е.; Романов, А.

    2015-01-01

    В статье рассматривается технология Bluetooth low energy и организация indoor-навигации и идентификации в зданиях с помощью портативных маячков. К области применения данной технологии можно отнести университетcкие кампусы, большие офисные здания, терминалы аэропортов, больницы и другие объекты. Цель проекта помочь людям ориентироваться в подобных зданиях с помощью средств программирования и мобильных устройств....

  16. SU-F-J-142: Proposed Method to Broaden Inclusion Potential of Patients Able to Use the Calypso Tracking System in Prostate Radiotherapy

    Energy Technology Data Exchange (ETDEWEB)

    Fiedler, D; Kuo, H; Bodner, W; Tome, W [Montefiore Medical Center, Bronx, NY (United States)

    2016-06-15

    Purpose: To introduce a non-standard method of patient setup, using BellyBoard immobilization, to better utilize the localization and tracking potential of an RF-beacon system with EBRT for prostate cancer. Methods: An RF-beacon phantom was imaged using a wide bore CT scanner, both in a standard level position and with a known rotation (4° pitch and 7.5° yaw). A commercial treatment planning system (TPS) was used to determine positional coordinates of each beacon, and the centroid of the three beacons for both setups. For each setup at the Linac, kV AP and Rt Lateral images were obtained. A full characterization of the RF-beacon system in clinical mode was completed for various beacons’ array-to-centroid distances, which includes vertical, lateral, and longitudinal offset data, as well as pitch and yaw offset measurements for the tilted phantom. For the single patient who has been setup using the proposed BellyBoard method, a supine simulation was first obtained. When abdominal protrusion was found to be exceeding the limits of the RF-Beacon system through distance-based analysis in the TPS, the patient is re-simulated prone with the BellyBoard. Array to centroid distance is measured again in the TPS, and if found to be within the localization or tracking region it is applied. Results: Characterization of limitations for the RF-beacon system in clinical mode showed acceptable consistency of offset determination for phantom setup accuracy. The nonstandard patient setup method reduced the beacons’ centroid-to-array distance by 8.32cm, from 25.13cm to 16.81cm; completely out of tracking range (greater than 20cm) to within setup tracking range (less than 20cm). Conclusion: Using the RF-beacon system in combination with this novel patient setup can allow patients who would otherwise not be candidates for beacon enhanced EBRT to now be able to benefit from the reduced PTV margins of this treatment method.

  17. SU-F-J-142: Proposed Method to Broaden Inclusion Potential of Patients Able to Use the Calypso Tracking System in Prostate Radiotherapy

    International Nuclear Information System (INIS)

    Fiedler, D; Kuo, H; Bodner, W; Tome, W

    2016-01-01

    Purpose: To introduce a non-standard method of patient setup, using BellyBoard immobilization, to better utilize the localization and tracking potential of an RF-beacon system with EBRT for prostate cancer. Methods: An RF-beacon phantom was imaged using a wide bore CT scanner, both in a standard level position and with a known rotation (4° pitch and 7.5° yaw). A commercial treatment planning system (TPS) was used to determine positional coordinates of each beacon, and the centroid of the three beacons for both setups. For each setup at the Linac, kV AP and Rt Lateral images were obtained. A full characterization of the RF-beacon system in clinical mode was completed for various beacons’ array-to-centroid distances, which includes vertical, lateral, and longitudinal offset data, as well as pitch and yaw offset measurements for the tilted phantom. For the single patient who has been setup using the proposed BellyBoard method, a supine simulation was first obtained. When abdominal protrusion was found to be exceeding the limits of the RF-Beacon system through distance-based analysis in the TPS, the patient is re-simulated prone with the BellyBoard. Array to centroid distance is measured again in the TPS, and if found to be within the localization or tracking region it is applied. Results: Characterization of limitations for the RF-beacon system in clinical mode showed acceptable consistency of offset determination for phantom setup accuracy. The nonstandard patient setup method reduced the beacons’ centroid-to-array distance by 8.32cm, from 25.13cm to 16.81cm; completely out of tracking range (greater than 20cm) to within setup tracking range (less than 20cm). Conclusion: Using the RF-beacon system in combination with this novel patient setup can allow patients who would otherwise not be candidates for beacon enhanced EBRT to now be able to benefit from the reduced PTV margins of this treatment method.

  18. A study on autonomous video navigation in close range with a cooperative target

    Science.gov (United States)

    Wei, Xiangquan; Guo, Maozu; Chen, Feng; Huang, Jianming; Zhang, Xuegong

    2009-10-01

    Autonomous service on orbit is a new developing trend of space service, in order to realize on orbit servicing, the problem of autonomous relative navigation needs to be solved. On the foundation of several typical schemes used by American, Russian as well as ESA etc., the main conception of our autonomous video navigation system is determined. The beacon system is composed of five beacon lamps, and has four invariant features relative to transformations of rotation, translation and scale. Two sets of double camera systems constituted of three fixed lens cameras with short focus are applied to the longer distance and the shorter distance respectively. Three kinds of measures are put forward to suppress the interference of miscellaneous lights. Then, the study and simulation of beacon recognition as well as the determination of relative positions and attitudes are expounded in this paper, the algorithm flow chart and corresponding simulation results are given. A kind of spiral capturing algorithm is used to increase the capturing efficiency, and the beacon recognition algorithm is designed according to the invariant features of beacon system to increase the recognition capability. In the determination of relative positions and attitudes, monocular algorithm and binocular algorithm are combined to ensure the reliability. Simulation results have verified the feasibility of the design of autonomous video navigation sensor and the autonomous video navigation techniques.

  19. Adaptive Source Localization Based Station Keeping of Autonomous Vehicles

    KAUST Repository

    Guler, Samet

    2016-10-26

    We study the problem of driving a mobile sensory agent to a target whose location is specied only in terms of the distances to a set of sensor stations or beacons. The beacon positions are unknown, but the agent can continuously measure its distances to them as well as its own position. This problem has two particular applications: (1) capturing a target signal source whose distances to the beacons are measured by these beacons and broadcasted to a surveillance agent, (2) merging a single agent to an autonomous multi-agent system so that the new agent is positioned at desired distances from the existing agents. The problem is solved using an adaptive control framework integrating a parameter estimator producing beacon location estimates, and an adaptive motion control law fed by these estimates to steer the agent toward the target. For location estimation, a least-squares adaptive law is used. The motion control law aims to minimize a convex cost function with unique minimizer at the target location, and is further augmented for persistence of excitation. Stability and convergence analysis is provided, as well as simulation results demonstrating performance and transient behavior.

  20. Reputation-based secure sensor localization in wireless sensor networks.

    Science.gov (United States)

    He, Jingsha; Xu, Jing; Zhu, Xingye; Zhang, Yuqiang; Zhang, Ting; Fu, Wanqing

    2014-01-01

    Location information of sensor nodes in wireless sensor networks (WSNs) is very important, for it makes information that is collected and reported by the sensor nodes spatially meaningful for applications. Since most current sensor localization schemes rely on location information that is provided by beacon nodes for the regular sensor nodes to locate themselves, the accuracy of localization depends on the accuracy of location information from the beacon nodes. Therefore, the security and reliability of the beacon nodes become critical in the localization of regular sensor nodes. In this paper, we propose a reputation-based security scheme for sensor localization to improve the security and the accuracy of sensor localization in hostile or untrusted environments. In our proposed scheme, the reputation of each beacon node is evaluated based on a reputation evaluation model so that regular sensor nodes can get credible location information from highly reputable beacon nodes to accomplish localization. We also perform a set of simulation experiments to demonstrate the effectiveness of the proposed reputation-based security scheme. And our simulation results show that the proposed security scheme can enhance the security and, hence, improve the accuracy of sensor localization in hostile or untrusted environments.

  1. A nonlinear OPC technique for laser beam control in turbulent atmosphere

    Science.gov (United States)

    Markov, V.; Khizhnyak, A.; Sprangle, P.; Ting, A.; DeSandre, L.; Hafizi, B.

    2013-05-01

    A viable beam control technique is critical for effective laser beam transmission through turbulent atmosphere. Most of the established approaches require information on the impact of perturbations on wavefront propagated waves. Such information can be acquired by measuring the characteristics of the target-scattered light arriving from a small, preferably diffraction-limited, beacon. This paper discusses an innovative beam control approach that can support formation of a tight laser beacon in deep turbulence conditions. The technique employs Brillouin enhanced fourwave mixing (BEFWM) to generate a localized beacon spot on a remote image-resolved target. Formation of the tight beacon doesn't require a wavefront sensor, AO system, or predictive feedback algorithm. Unlike conventional adaptive optics methods which allow wavefront conjugation, the proposed total field conjugation technique is critical for beam control in the presence of strong turbulence and can be achieved by using this non-linear BEFWM technique. The phase information retrieved from the established beacon beam can then be used in conjunction with an AO system to propagate laser beams in deep turbulence.

  2. Adaptive optics compensation using active illumination

    Science.gov (United States)

    Higgs, Charles; Barclay, Herbert T.; Kansky, Jan E.; Murphy, Daniel V.; Primmerman, Charles A.

    1998-09-01

    We have conducted atmospheric compensation experiments using active illumination for both adaptive-optics and tracking. Tests were performed in support of the Air Force's Airborne Laser program. The tests utilized the 5.4-km horizontal propagation range at the Lincoln Laboratory Firepond facility. The adaptive-optics beacon was provided by actively illuminating the target with a multibeam laser illuminator. A second multibeam laser illuminator was used to provide a beacon for an imaging tracker. Experiments were conducted using two different adaptive-optics illuminator configurations, as well as with point-source beacons. Data were collected over a range of atmospheric conditions. Results from these tests have helped to provide a performance benchmark for the Airborne Laser program.

  3. Mobile robot trajectory tracking using noisy RSS measurements: an RFID approach.

    Science.gov (United States)

    Miah, M Suruz; Gueaieb, Wail

    2014-03-01

    Most RF beacons-based mobile robot navigation techniques rely on approximating line-of-sight (LOS) distances between the beacons and the robot. This is mostly performed using the robot's received signal strength (RSS) measurements from the beacons. However, accurate mapping between the RSS measurements and the LOS distance is almost impossible to achieve in reverberant environments. This paper presents a partially-observed feedback controller for a wheeled mobile robot where the feedback signal is in the form of noisy RSS measurements emitted from radio frequency identification (RFID) tags. The proposed controller requires neither an accurate mapping between the LOS distance and the RSS measurements, nor the linearization of the robot model. The controller performance is demonstrated through numerical simulations and real-time experiments. ©2013 Published by ISA. All rights reserved.

  4. a Statistical Analysis on the System Performance of a Bluetooth Low Energy Indoor Positioning System in a 3d Environment

    Science.gov (United States)

    Haagmans, G. G.; Verhagen, S.; Voûte, R. L.; Verbree, E.

    2017-09-01

    Since GPS tends to fail for indoor positioning purposes, alternative methods like indoor positioning systems (IPS) based on Bluetooth low energy (BLE) are developing rapidly. Generally, IPS are deployed in environments covered with obstacles such as furniture, walls, people and electronics influencing the signal propagation. The major factor influencing the system performance and to acquire optimal positioning results is the geometry of the beacons. The geometry of the beacons is limited to the available infrastructure that can be deployed (number of beacons, basestations and tags), which leads to the following challenge: Given a limited number of beacons, where should they be placed in a specified indoor environment, such that the geometry contributes to optimal positioning results? This paper aims to propose a statistical model that is able to select the optimal configuration that satisfies the user requirements in terms of precision. The model requires the definition of a chosen 3D space (in our case 7 × 10 × 6 meter), number of beacons, possible user tag locations and a performance threshold (e.g. required precision). For any given set of beacon and receiver locations, the precision, internal- and external reliability can be determined on forehand. As validation, the modeled precision has been compared with observed precision results. The measurements have been performed with an IPS of BlooLoc at a chosen set of user tag locations for a given geometric configuration. Eventually, the model is able to select the optimal geometric configuration out of millions of possible configurations based on a performance threshold (e.g. required precision).

  5. Action Research on Development and Application of Internet of Things Services in Hospital.

    Science.gov (United States)

    Park, Arum; Chang, Hyejung; Lee, Kyoung Jun

    2017-01-01

    Services based on the Internet of Things (IoT) technologies have emerged in various business environments. To enhance health service quality and maximize benefits, this study applied an IoT technology based on NFC and iBeacon as an omni-channel service for patient care in hospitals. Application of the IoT technology based on NFC and iBeacon was conducted in a general hospital during August 2015 through June 2016, and the development and evaluation results were aligned to an action research framework. The five phases in the action research included diagnosing, planning action, taking action, evaluating action, and specifying learning phases. During the first two phases, problems of functional operations in a hospital were diagnosed and eight service models were designed by using iBeacon and NFC to solve the problems. Service models were applied to the hospital by installing beacons, wearable beacons, beacon scanners, and NFC tags during the third phase. During the fourth and fifth phases, the roles and benefits of stakeholders participating in the service models were evaluated, and issues and knowledge of the whole application process were derived and summarized from technological, economic, social and legal perspectives, respectively. From an action research perspective, IoT-based healthcare services were developed and verified. IoT-based services enable the hospital to acquire lifelog data for precision medicine and ultimately be able to go one step closer to precision medical care. The derived service models could provide patients more enhanced healthcare services and improve the work efficiency and effectiveness of the hospital.

  6. Simultaneous detection of mRNA and protein stem cell markers in live cells

    Directory of Open Access Journals (Sweden)

    Bao Gang

    2009-04-01

    Full Text Available Abstract Background Biological studies and medical application of stem cells often require the isolation of stem cells from a mixed cell population, including the detection of cancer stem cells in tumor tissue, and isolation of induced pluripotent stem cells after eliciting the expression of specific genes in adult cells. Here we report the detection of Oct-4 mRNA and SSEA-1 protein in live carcinoma stem cells using respectively molecular beacon and dye-labeled antibody, aiming to establish a new method for stem cells detection and isolation. Results Quantification of Oct-4 mRNA and protein in P19 mouse carcinoma stem cells using respectively RT-PCR and immunocytochemistry confirmed that their levels drastically decreased after differentiation. To visualize Oct-4 mRNA in live stem cells, molecular beacons were designed, synthesized and validated, and the detection specificity was confirmed using control studies. We found that the fluorescence signal from Oct-4-targeting molecular beacons provides a clear discrimination between undifferentiated and retinoic acid-induced differentiated cells. Using deconvolution fluorescence microscopy, Oct-4 mRNAs were found to reside on one side of the cytosol. We demonstrated that, using a combination of Oct-4 mRNA-targeting molecular beacon with SSEA-1 antibody in flow cytometric analysis, undifferentiated stem cells can be clearly distinguished from differentiated cells. We revealed that Oct-4 targeting molecular beacons do not seem to affect stem cell biology. Conclusion Molecular beacons have the potential to provide a powerful tool for highly specific detection and isolation of stem cells, including cancer stem cells and induced pluripotent stem (iPS cells without disturbing cell physiology. It is advantageous to perform simultaneous detection of intracellular (mRNA and cell-surface (protein stem cell markers in flow cytometric analysis, which may lead to high detection sensitivity and efficiency.

  7. A STATISTICAL ANALYSIS ON THE SYSTEM PERFORMANCE OF A BLUETOOTH LOW ENERGY INDOOR POSITIONING SYSTEM IN A 3D ENVIRONMENT

    Directory of Open Access Journals (Sweden)

    G. G. Haagmans

    2017-09-01

    Full Text Available Since GPS tends to fail for indoor positioning purposes, alternative methods like indoor positioning systems (IPS based on Bluetooth low energy (BLE are developing rapidly. Generally, IPS are deployed in environments covered with obstacles such as furniture, walls, people and electronics influencing the signal propagation. The major factor influencing the system performance and to acquire optimal positioning results is the geometry of the beacons. The geometry of the beacons is limited to the available infrastructure that can be deployed (number of beacons, basestations and tags, which leads to the following challenge: Given a limited number of beacons, where should they be placed in a specified indoor environment, such that the geometry contributes to optimal positioning results? This paper aims to propose a statistical model that is able to select the optimal configuration that satisfies the user requirements in terms of precision. The model requires the definition of a chosen 3D space (in our case 7 × 10 × 6 meter, number of beacons, possible user tag locations and a performance threshold (e.g. required precision. For any given set of beacon and receiver locations, the precision, internal- and external reliability can be determined on forehand. As validation, the modeled precision has been compared with observed precision results. The measurements have been performed with an IPS of BlooLoc at a chosen set of user tag locations for a given geometric configuration. Eventually, the model is able to select the optimal geometric configuration out of millions of possible configurations based on a performance threshold (e.g. required precision.

  8. The primary care amplification model: taking the best of primary care forward

    Directory of Open Access Journals (Sweden)

    Nicholson Caroline

    2008-12-01

    Full Text Available Abstract Background Primary care internationally is approaching a new paradigm. The change agenda implicit in this threatens to de-stabilise and challenge established general practice and primary care. Discussion The Primary Care Amplification Model offers a means to harness the change agenda by 'amplifying' the strengths of established general practices around a 'beacon' practice. Conclusion Such 'beacon' practices can provide a mustering point for an expanded scope of practice for primary care, integrated primary/secondary service delivery, interprofessional learning, relevant local clinical research, and a focus on local service innovation, enhancing rather than fragmenting the collective capacity of existing primary care.

  9. Desenvolupament d'una plataforma d'anuncis geolocalitzats

    OpenAIRE

    Martin Alaminos, David

    2017-01-01

    Hem desenvolupat un sistema capaç de determinar el posicionament dels anuncis que emeten uns beacons. Es tracta d'un pipeline de dades compost per un algorisme de subhasta pels anunciants, un estimador del rendiment dels anuncis mitjançant machine learning i una agregació d'ambdós resultats. We developed a system capable of establishing the positioning of advertisements broadcast by a set of beacons. It is a data pipeline consisting in an auction algorithm for advertisers, a performance es...

  10. Nearly Cyclic Pursuit and its Hierarchical variant for Multi-agent Systems

    DEFF Research Database (Denmark)

    Iqbal, Muhammad; Leth, John-Josef; Ngo, Trung Dung

    2015-01-01

    The rendezvous problem for multiple agents under nearly cyclic pursuit and hierarchical nearly cyclic pursuit is discussed in this paper. The control law designed under nearly cyclic pursuit strategy enables the agents to converge at a point dictated by a beacon. A hierarchical version of the nea......The rendezvous problem for multiple agents under nearly cyclic pursuit and hierarchical nearly cyclic pursuit is discussed in this paper. The control law designed under nearly cyclic pursuit strategy enables the agents to converge at a point dictated by a beacon. A hierarchical version...

  11. Identifying Anomalous Port-Specific Network Behavior

    Science.gov (United States)

    2010-05-01

    Backscatter 4.3 6.2 1.3 Connection Attempt 4.3 7.6 3.3 User Non-Web activity 3.9 22.0 11.9 SYN flood 3.6 9.0 4.4 Beaconing (Non-443) 3.6 20.5 5.0 Large...flagged events are composed of suspicious events such as beaconing (keep- alives on non-standard ports), SYN floods , scanning, and large data...systems. A port is an integer value between 0 and 65535 that represents a logical connection place for Transmission Control Protocol ( TCP ) and User

  12. The National Association for Girls and Women in Sport: 110 Years of Promoting Social Justice and Change

    Science.gov (United States)

    Ladda, Shawn

    2009-01-01

    From writing the first Guidebooks for hockey, soccer, swimming, track and field, and basketball, to lobbying Congress to strive for equity and equal opportunities for girls, the National Association for Girls and Women in Sport (NAGWS) has been and continues to be the beacon in education to advance fairness and equity in sports. As NAGWS enters…

  13. JSATS Decoder Software Manual

    Energy Technology Data Exchange (ETDEWEB)

    Flory, Adam E.; Lamarche, Brian L.; Weiland, Mark A.

    2013-05-01

    The Juvenile Salmon Acoustic Telemetry System (JSATS) Decoder is a software application that converts a digitized acoustic signal (a waveform stored in the .bwm file format) into a list of potential JSATS Acoustic MicroTransmitter (AMT) tagcodes along with other data about the signal including time of arrival and signal to noise ratios (SNR). This software is capable of decoding single files, directories, and viewing raw acoustic waveforms. When coupled with the JSATS Detector, the Decoder is capable of decoding in ‘real-time’ and can also provide statistical information about acoustic beacons placed within receive range of hydrophones within a JSATS array. This document details the features and functionality of the software. The document begins with software installation instructions (section 2), followed in order by instructions for decoder setup (section 3), decoding process initiation (section 4), then monitoring of beacons (section 5) using real-time decoding features. The last section in the manual describes the beacon, beacon statistics, and the results file formats. This document does not consider the raw binary waveform file format.

  14. 14 CFR 171.273 - Maintenance and operations requirements.

    Science.gov (United States)

    2010-01-01

    ... localizer course alignment, width, and clearance, glide path elevation angle and course width, and marker... emission and frequencies of the ISMLS localizer, glide path, beacon markers, and associated compass... owner or his maintenance representative must provide a sufficient stock of spare parts, including solid...

  15. A Storied Past: The Best Tales are Often Found Right inside Your Own Front Door

    Science.gov (United States)

    Elliott, Zetta

    2011-01-01

    In this article, the author talks about her past experiences and how she immersed herself in African-American literature. While teaching a journalism class in an after-school program at the Decatur Clearpool Beacon School in Brooklyn's Bed-Stuy section, the author realized that most of her students had no sense of African-American history beyond a…

  16. 75 FR 79293 - Amendment and Revocation of Class E Airspace; Vero Beach, FL

    Science.gov (United States)

    2010-12-20

    ... Federal Aviation Administration 14 CFR Part 71 Amendment and Revocation of Class E Airspace; Vero Beach... removes Class E airspace designated as an extension to Class D surface area at Vero Beach Municipal Airport, Vero Beach, FL. The Vero Beach Non-Directional Beacon (NDB) has been decommissioned and new...

  17. 75 FR 65581 - Proposed Amendment and Revocation of Class E Airspace, Vero Beach, FL

    Science.gov (United States)

    2010-10-26

    ... Beach, FL AGENCY: Federal Aviation Administration (FAA), DOT. ACTION: Notice of proposed rulemaking... surface area at Vero Beach Municipal Airport, Vero Beach, FL. The Vero Beach Non- Directional Beacon (NDB... reference to the decommissioned Vero Beach NDB at Vero Beach Municipal Airport, Vero Beach, FL. This action...

  18. Two books on nuclear disasters

    OpenAIRE

    森, 祐二

    1981-01-01

    Nuclear Disasters in the UraIs by Zhores A. Medvedev, Translated by George Saunders, W.W. Norton & Company, New York. 1979. 214pp. Atomic Soldiers, American Victims of Nuclear Experiments by Howard L. Rosenberg, Beacon Press, Boston, 1980. 192pp.

  19. Measurement of the group velocity of light in sea water at the ANTARES site

    NARCIS (Netherlands)

    Adrian-Martinez, S.; Al Samarai, I.; Albert, A.; Andre, M.; Anghinolfi, M.; Anton, G.; Anvar, S.; Ardid, M.; Jesus, A.C.A.; Astraatmadja, T.; Aubert, J.J.; Baret, B.; Basa, S.; Bertin, V.; Biagi, S.; Bigi, A.; Bigongiari, C.; Bogazzi, C.; Bou-Cabo, M.; Bouhou, B.; Bouwhuis, M.C.; Brunner, J.; Busto, J.; Camarena, F.; Capone, A.; Carloganu, C.; Carminati, G.; Carr, J.; Cecchini, S.; Charif, Z.; Charvis, P.; Chiarusi, T.; Circella, M.; Costantini, H.; Coyle, P.; Curtil, C.; De Bonis, G.; Decowski, M.P.; Dekeyser, I.; Deschamps, A.; Distefano, C.; Donzaud, C.; Dornic, D.; Dorosti, Q.; Drouhin, D.; Eberl, T.; Emanuele, U.; Enzenhofer, A.; Ernenwein, J.P.; Escoffier, S.; Fermani, P.; Ferri, M.; Flaminio, V.; Folger, F.; Fritsch, U.; Fuda, J.L.; Galata, S.; Gay, P.; Geyer, K.; Giacomelli, G.; Giordano, V.; Gomez-Gonzalez, J.P.; Graf, K.; Guillard, G.; Halladjian, G.; Hallewell, G.; van Haren, H.; Hartman, J.; Heijboer, A.J.; Hello, Y.; Hernandez-Rey, J.J.; Herold, B.; Hossl, J.; Hsu, C.C.; De Jong, M.; Kadler, M.; Kalekin, O.; Kappes, A.; Katz, U.; Kavatsyuk, O.; Kooijman, P.; Kopper, C.; Kouchner, A.; Kreykenbohm, I.; Kulikovskiy, V.; Lahmann, R.; Lamare, P.; Larosa, G.; Lattuada, D.; Lefevre, D.; Lim, G.; Lo Presti, D.; Loehner, H.; Loucatos, S.; Mangano, S.; Marcelin, M.; Margiotta, A.; Martinez-Mora, J.A.; McMillan, J.E.; Meli, A.; Montaruli, T.; Moscoso, L.; Motz, H.; Neff, M.; Nezri, E.; Palioselitis, D.; Pavalas, G.E.; Payet, K.; Payre, P.; Petrovic, J.; Piattelli, P.; Picot-Clemente, N.; Popa, V.; Pradier, T.; Presani, E.; Racca, C.; Reed, C.; Riccobene, G.; Richardt, C.; Richter, R.; Riviere, C.; Robert, A.; Roensch, K.; Rostovtsev, A.; Ruiz-Rivas, J.; Rujoiu, M.; Russo, G.V.; Salesa, F.; Samtleben, D.F.E.; Sapienza, P.; Schock, F.; Schuller, J.P.; Schussleraf, F.; Seitz, T.; Shanidze, R.; Simeone, F.; Spies, A.; Spurio, M.; Steijger, J.J.M.; Stolarczyk, T.; Sanchez-Losa, A.; Taiuti, M.; Tamburini, C.; Thompson, L.F.; Toscano, S.; Vallage, B.; Van Elewyck, V.; Vannoni, G.; Vecchi, M.; Vernin, P.; Wagner, S.; Wijnker, G.; Wilms, J.; de Wolf, E.; Yepes, H.; Zaborov, D.; Zornoza, J.D.; Zuniga, J.

    2012-01-01

    The group velocity of light has been measured at eight different wavelengths between 385 nm and 532 nm in the Mediterranean Sea at a depth of about 2.2 km with the ANTARES optical beacon systems. A parametrisation of the dependence of the refractive index on wavelength based on the salinity,

  20. Re-imagining South African Cultural Studies

    African Journals Online (AJOL)

    was used as a genre which could capture a complexity of access to identity, a more acute psychological ..... kwaito music (forthcoming in a chapter by Simon Stephens in Openings“), which uses a bricolage of the ..... Evolution of Society, translated and introduced by T. McCarthy, Beacon, Boston. Hamilton, C. 1998. Terrific ...

  1. 76 FR 72442 - Certain Automotive GPS Navigation Systems, Components Thereof, and Products Containing Same...

    Science.gov (United States)

    2011-11-23

    ... Tariff Act of 1930, as amended, 19 U.S.C. 1337, on behalf of Beacon Navigation GmbH of Switzerland. A... further alleges that an industry in the United States exists as required by subsection (a)(2) of section... '511 patent, and whether an industry in the United States exists as required by subsection (a)(2) of...

  2. 77 FR 45239 - Amendment of Class E Airspace; Bar Harbor, ME

    Science.gov (United States)

    2012-07-31

    ...-1366; Airspace Docket No. 11-ANE-13] Amendment of Class E Airspace; Bar Harbor, ME AGENCY: Federal... area at Bar Harbor, ME, as the Surry Non-Directional Radio Beacon (NDB) has been decommissioned and new Standard Instrument Approach Procedures have been developed at Hancock County-Bar Harbor Airport. This...

  3. 77 FR 27666 - Proposed Amendment of Class E Airspace; Bar Harbor, ME

    Science.gov (United States)

    2012-05-11

    ...-1366; Airspace Docket No. 11-ANE-13] Proposed Amendment of Class E Airspace; Bar Harbor, ME AGENCY... action proposes to amend Class E Airspace at Bar Harbor, ME, as the Surry Non-Directional Radio Beacon... Hancock County-Bar Harbor Airport. This action would enhance the safety and airspace management of...

  4. Ranging energy optimization for robust sensor positioning based on semidefinite programming

    NARCIS (Netherlands)

    Wang, T.; Leus, G.; Huang, L.

    2009-01-01

    Sensor positioning is an important task of location-aware wireless sensor networks. In most sensor positioning systems, sensors and beacons need to emit ranging signals to each other. Sensor ranging energy should be low to prolong system lifetime, but sufficiently high to fulfill prescribed accuracy

  5. Beebe isn't 'boo boo' any more. In a 'readers' market, magazine helps change public perception.

    Science.gov (United States)

    Botvin, Judith D

    2003-01-01

    Beebe Medical Center, Lewes, Del., was referred to by many of its neighbors as "Booboo Hospital," but all that changed when Sharon Perry, director of public relations, created the Beacon, a magazine ideally suited to her market that is largely retirees and people ages 55 and older.

  6. 78 FR 1838 - Takes of Marine Mammals Incidental to Specified Activities; St. George Reef Light Station...

    Science.gov (United States)

    2013-01-09

    ... reasonably likely to, adversely affect the species or stock through effects on annual rates of recruitment or... a crew of two to three people to the Station by helicopter to repair the beacon light. For each... stock distributed among sites in Alaska, British Columbia; and a San Miguel Island stock distributed...

  7. 76 FR 79157 - Takes of Marine Mammals Incidental to Specified Activities; St. George Reef Light Station...

    Science.gov (United States)

    2011-12-21

    ... species or stock through effects on annual rates of recruitment or survival.'' Section 101(a)(5)(D) of the... people to the Station by helicopter to repair the beacon light. For each emergency repair event, the..., British Columbia; and a San Miguel Island stock distributed along the west coast of the continental U.S...

  8. 75 FR 80471 - Takes of Marine Mammals Incidental to Specified Activities; St. George Reef Light Station...

    Science.gov (United States)

    2010-12-22

    ... species or stock through effects on annual rates of recruitment or survival.'' Section 101(a)(5)(D) of the... SGRLPS proposes to send a crew of two to three people to the Station by helicopter to repair the beacon... distributed among sites in Alaska, British Columbia; and a San Miguel Island stock distributed along the west...

  9. 78 FR 27247 - Center for Scientific Review; Notice of Closed Meetings

    Science.gov (United States)

    2013-05-09

    ...; Notice of Closed Meetings Pursuant to section 10(d) of the Federal Advisory Committee Act, as amended (5... grant applications. Place: The Allerton Hotel, 701 North Michigan Avenue, Chicago, IL 60611. Contact...:00 p.m. Agenda: To review and evaluate grant applications. Place: Beacon Hotel and Corporate Quarters...

  10. Implementation and verification of an advancement to the Galileo system search and rescue service signal simulator

    Science.gov (United States)

    Lin, Mo; Wu, Qiongzhi; Liu, Shaobo

    2009-12-01

    Based on the Sino-European cooperation in the Galileo satellite navigation system Search And Rescue(SAR) service, a multi-channel distress beacon signal simulator is presented here. The mathematic model and spectrum analysis of current 406 MHz distress beacons signals is presented which includes Emergency Position Indicating Radio Beacons (EPIRB) for Maritime, Personal Locator Beacons (PLB) for land (road and rail), and Emergency Locator Transmitter (ELT) for aeronautical applications. Based on Software Defined Radio (SDR) and digital IF techniques, the design of a SAR Signal Simulator (SAR-SS) is proposed, which can generate no less than 20 distress signals simultaneously. The Doppler shift and space propagation effect such as ionosphere delay, free space attenuation and etc, are simulated in SAR-SS, which provided a significant test and evaluation capability for SAR/Galileo project. The performance of SAR-SS is more accurate and stabile than the Cospas-Sarsat(C-S) requirement. SAR-SS will be a significant instrument for the Galileo system search and rescue project ground test.

  11. 32 CFR 707.7 - Submarine identification light.

    Science.gov (United States)

    2010-07-01

    ... 32 National Defense 5 2010-07-01 2010-07-01 false Submarine identification light. 707.7 Section... RULES WITH RESPECT TO ADDITIONAL STATION AND SIGNAL LIGHTS § 707.7 Submarine identification light. Submarines may display, as a distinctive means of identification, an intermittent flashing amber beacon with...

  12. Ultrasound-Aided Pedestrian Dead Reckoning for Indoor Navigation

    NARCIS (Netherlands)

    Fischer, C.; Kavitha Muthukrishnan, K.; Hazas, M.; Gellersen, H.

    2008-01-01

    Ad hoc solutions for tracking and providing navigation support to emergency response teams is an important and safety-critical challenge. We propose a navigation system based on a combination of foot-mounted inertial sensors and ultrasound beacons. We evaluate experimentally the performance of our

  13. Dead Reckoning Localization Technique for Mobile Wireless Sensor Networks

    OpenAIRE

    Rashid, Haroon; Turuk, Ashok Kumar

    2015-01-01

    Full paper at: http://arxiv.org/pdf/1504.06797.pdfThis paper shows how a mobile node can be localized with less than three beacon nodes. It uses a technique known as dead-reckoning for localization of nodes at specific intervals

  14. Topological properties of random wireless networks

    Indian Academy of Sciences (India)

    ). Finally, in § 5 we provide an annotated .... using independent exponential spacings as follows. Let Yn,0,Yn,1,... ,Yn,n be i.i.d. ...... by the sensors or beacon nodes that help in the self organization of the network. The subgraphs specified by W ...

  15. 76 FR 5300 - Proposed Amendment of Class E Airspace; Cable Union, WI

    Science.gov (United States)

    2011-01-31

    ... Federal Aviation Administration 14 CFR Part 71 Proposed Amendment of Class E Airspace; Cable Union, WI...: This action proposes to amend Class E airspace at Cable Union, WI. Decommissioning of the Seely non-directional beacon (NDB) at Cable Union Airport, Cable Union, WI, has made this action necessary for the...

  16. 76 FR 22014 - Amendment of Class E Airspace; Cable Union, WI

    Science.gov (United States)

    2011-04-20

    ... Federal Aviation Administration 14 CFR Part 71 Amendment of Class E Airspace; Cable Union, WI AGENCY... airspace for Cable Union, WI. Decommissioning of the Seely non-directional beacon (NDB) at Cable Union Airport, Cable Union, WI, has made this action necessary to enhance the safety and management of...

  17. Higher Education and the Discursive Construction of American National Identity, 1946-2013

    Science.gov (United States)

    Palmadessa, Allison L.

    2014-01-01

    American institutions of higher education have served as a beacon of American idealism and identity since the foundation of the earliest universities. As the nation developed, higher education matured and continued to maintain a position of importance in the future of the nation. While the university has perpetuated a national cultural identity,…

  18. 77 FR 4458 - Amendment of Class E Airspace; Rugby, ND

    Science.gov (United States)

    2012-01-30

    ...-0433; Airspace Docket No. 11-AGL-12] Amendment of Class E Airspace; Rugby, ND AGENCY: Federal Aviation Administration (FAA), DOT. ACTION: Final rule. SUMMARY: This action amends Class E airspace for Rugby, ND. Decommissioning of the Rugby non-directional beacon (NDB) at Rugby Municipal Airport has made this action...

  19. 76 FR 66870 - Proposed Amendment of Class E Airspace; Rugby, ND

    Science.gov (United States)

    2011-10-28

    ...-0433; Airspace Docket No. 11-AGL-12] Proposed Amendment of Class E Airspace; Rugby, ND AGENCY: Federal... proposes to amend Class E airspace at Rugby, ND. Decommissioning of the Rugby non-directional beacon (NDB) at Rugby Municipal Airport, Rugby, ND, has made this action necessary for the safety and management...

  20. The influence of hand-held information and communication technology on visitor perceptions of risk and risk-related behavior

    Science.gov (United States)

    Steven R. Martin; Kristen Pope

    2012-01-01

    As devices like personal locator beacons become more readily available, more visitors may bring them into wilderness and use them to request rescues and may develop unrealistic expectations of rescue. In an exploratory study in 2009, 235 overnight visitors to the King Range Wilderness in California completed a written survey. Of the respondents, 40 percent considered...

  1. 50 CFR 600.746 - Observers.

    Science.gov (United States)

    2010-10-01

    ... South Pacific Tuna Act of 1988 (16 U.S.C. 973 et seq.), or any other U.S. law. (b) Observer safety. An... USCG regulations: (1) Personal flotation devices/ immersion suits; (2) Ring buoys; (3) Distress signals; (4) Fire extinguishing equipment; (5) Emergency position indicating radio beacon (EPIRB), when...

  2. Making Watches or Making Music.

    Science.gov (United States)

    Houston, Paul D.

    1994-01-01

    Denis Doyle's proposal in the same "Kappan" issue is part of the recent campaign to persuade the public to privatize public education. Although some view private schools as beacons of transformation, the former are considerably less creative and diverse than public schools. Private companies will do no better than (underfunded) public agencies at…

  3. Vibration control for the ARGOS laser launch path

    Science.gov (United States)

    Peter, Diethard; Gässler, Wolfgang; Borelli, Jose; Barl, Lothar; Rabien, S.

    2012-07-01

    Present and future adaptive optics systems aim for the correction of the atmospheric turbulence over a large field of view combined with large sky coverage. To achieve this goal the telescope is equipped with multiple laser beacons. Still, to measure tip-tilt aberrations a natural guide star is used. For some fields such a tilt-star is not available and a correction on the laser beacons alone is applied. For this method to work well the laser beacons must not be affected by telescope vibrations on their up-link path. For the ARGOS system the jitter of the beacons is specified to be below 0.05. To achieve this goal a vibration compensation system is necessary to mitigate the mechanical disturbances. The ARGOS vibration compensation system is an accelerometer based feed forward system. The accelerometer measurements are fed into a real time controller. To achieve high performance the controller of the system is model based. The output is applied to a fast steering mirror. This paper presents the concept of the ARGOS vibration compensation, the hardware, and laboratory results.

  4. 76 FR 53356 - Proposed Amendment of Class E Airspace; Greenfield, IA

    Science.gov (United States)

    2011-08-26

    ...-0846; Airspace Docket No. 11-ACE-18] Proposed Amendment of Class E Airspace; Greenfield, IA AGENCY... action proposes to amend Class E airspace at Greenfield, IA. Decommissioning of the Greenfield non-directional beacon (NDB) at Greenfield Municipal Airport, Greenfield, IA, has made this action necessary for...

  5. 77 FR 50650 - Proposed Amendment of Class E Airspace; Boone, IA

    Science.gov (United States)

    2012-08-22

    ...-1432; Airspace Docket No. 11-ACE-25] Proposed Amendment of Class E Airspace; Boone, IA AGENCY: Federal... proposes to amend Class E airspace at Boone, IA. Decommissioning of the Boone non-directional beacon (NDB... instrument approach procedures at Boone Municipal Airport, Boone, IA. Airspace reconfiguration is necessary...

  6. 77 FR 50647 - Proposed Amendment of Class E Airspace; Perry, IA

    Science.gov (United States)

    2012-08-22

    ...-1435; Airspace Docket No. 11-ACE-28] Proposed Amendment of Class E Airspace; Perry, IA AGENCY: Federal... proposes to amend Class E airspace at Perry, IA. Decommissioning of the Perry non-directional beacon (NDB) at Perry Municipal Airport, Perry, IA, has made reconfiguration necessary for standard instrument...

  7. 78 FR 18800 - Amendment of Class E Airspace; Decorah, IA

    Science.gov (United States)

    2013-03-28

    ...-1433; Airspace Docket No. 11-ACE-26] Amendment of Class E Airspace; Decorah, IA AGENCY: Federal... Decorah, IA. Decommissioning of the Decorah non-directional beacon (NDB) at Decorah Municipal Airport has... Register a notice of proposed rulemaking (NPRM) to amend Class E airspace for the Decorah, IA, area...

  8. 77 FR 68682 - Amendment of Class E Airspace; Guthrie, IA

    Science.gov (United States)

    2012-11-16

    ...-1436; Airspace Docket No. 11-ACE-29] Amendment of Class E Airspace; Guthrie, IA AGENCY: Federal... Guthrie, IA. Decommissioning of the Guthrie Center non-directional radio beacon (NDB) at Guthrie County... proposed rulemaking (NPRM) to amend Class E airspace for the Guthrie, IA, area, creating additional...

  9. 77 FR 4459 - Amendment of Class E Airspace; Greenfield, IA

    Science.gov (United States)

    2012-01-30

    ...-0846; Airspace Docket No. 11-ACE-18] Amendment of Class E Airspace; Greenfield, IA AGENCY: Federal... Greenfield, IA. Decommissioning of the Greenfield non-directional beacon (NDB) at Greenfield Municipal... rulemaking to amend Class E airspace for Greenfield, IA, reconfiguring controlled airspace at Greenfield...

  10. 76 FR 53353 - Proposed Amendment of Class E Airspace; Carroll, IA

    Science.gov (United States)

    2011-08-26

    ...-0845; Airspace Docket No. 11-ACE-19] Proposed Amendment of Class E Airspace; Carroll, IA AGENCY... action proposes to amend Class E airspace at Carroll, IA. Decommissioning of the Carroll non-directional beacon (NDB) at Arthur N. Neu Airport, Carroll, IA, has made this action necessary for the safety and...

  11. 75 FR 37292 - Amendment of Class E Airspace; Cherokee, IA

    Science.gov (United States)

    2010-06-29

    ...-0085; Airspace Docket No. 10-ACE-1] Amendment of Class E Airspace; Cherokee, IA AGENCY: Federal... Cherokee, IA. Decommissioning of the Pilot Rock non-directional beacon (NDB) at Cherokee County Regional Airport, Cherokee, IA has made this action necessary to enhance the safety and management of Instrument...

  12. 78 FR 76053 - Amendment of Class E Airspace; Chariton, IA

    Science.gov (United States)

    2013-12-16

    ...-0255; Airspace Docket No. 13-ACE-4] Amendment of Class E Airspace; Chariton, IA AGENCY: Federal... Chariton, IA. Decommissioning of the Chariton non-directional beacon (NDB) at Chariton Municipal Airport... Register a notice of proposed rulemaking (NPRM) to amend Class E airspace for the Chariton, IA, area...

  13. 76 FR 73501 - Amendment of Class E Airspace; Carroll, IA

    Science.gov (United States)

    2011-11-29

    ...-0845; Airspace Docket No. 11-ACE-19] Amendment of Class E Airspace; Carroll, IA AGENCY: Federal... Carroll, IA. Decommissioning of the Carroll non-directional beacon (NDB) at Arthur N. Neu Airport, Carroll, IA, has made this action necessary to enhance the safety and management of Instrument Flight Rule...

  14. 78 FR 18798 - Amendment of Class E Airspace; West Union, IA

    Science.gov (United States)

    2013-03-28

    ...-1434; Airspace Docket No. 11-ACE-27] Amendment of Class E Airspace; West Union, IA AGENCY: Federal... West Union, IA. Decommissioning of the West Union non-directional beacon (NDB) at George L. Scott... Federal Register a notice of proposed rulemaking (NPRM) to amend Class E airspace for the West Union, IA...

  15. 77 FR 45987 - Proposed Amendment of Class E Airspace; Guthrie, IA

    Science.gov (United States)

    2012-08-02

    ...-1436; Airspace Docket No. 11-ACE-29] Proposed Amendment of Class E Airspace; Guthrie, IA AGENCY... action proposes to amend Class E airspace at Guthrie, IA. Decommissioning of the Guthrie Center non-directional radio beacon (NDB) at Guthrie County Regional Airport, Guthrie, IA, has made this action necessary...

  16. 76 FR 75447 - Amendment of Class E Airspace; Centerville, IA

    Science.gov (United States)

    2011-12-02

    ...-0830; Airspace Docket No. 11-ACE-16] Amendment of Class E Airspace; Centerville, IA AGENCY: Federal... Centerville, IA. Decommissioning of the Centerville non-directional beacon (NDB) and cancellation of the NDB... Federal Register a notice of proposed rulemaking to amend Class E airspace for the Centerville, IA, area...

  17. 76 FR 1513 - Amendment of Class E Airspace; Horseshoe Bay, TX

    Science.gov (United States)

    2011-01-11

    ... Horseshoe Bay, TX. Decommissioning of the Horseshoe Bay Resort non-directional beacon (NDB) at Horseshoe Bay Resort Airport, Horseshoe Bay, TX, has made this action necessary to enhance the safety and management of... Horseshoe Bay Resort Airport (75 FR 66013) Docket No. FAA-2010-0843. Interested parties were invited to...

  18. 75 FR 66013 - Proposed Amendment of Class E Airspace; Horseshoe Bay, TX

    Science.gov (United States)

    2010-10-27

    ... Resort non-directional beacon (NDB) at Horseshoe Bay Resort Airport, Horseshoe Bay, TX, has made this action necessary for the safety and management of Instrument Flight Rules (IFR) operations at the airport... from 700 feet above the surface for standard instrument approach procedures at Horseshoe Bay Resort...

  19. Accuracy enhancement of navigation images using blind restoration method

    Science.gov (United States)

    Zhao, Jianhui; Zhang, Cundu; Yu, Tao; Li, Fan

    2018-01-01

    Optical navigation is one of the most promising technologies in the area of deep space autonomous navigation. However, since the optical images are strict motion blurred images, it is difficult to extract the lines of sight (LOS) to the beacons to reckon the spacecraft attitude and orbital position during the deep space cruise phase. This paper proposes a new blind restoration approach to effectively recover the clear image. We use a modified median filter to eliminate the black and white noises, and also, construct a blind estimation model, built on the sparsity of the gradient of navigation image, to estimate the global point spread function (PSF). Moreover, we select a few bright beacons to recover the motion blurred image, where the average value of the beacon centroid is adopted to calculate the relative position for the optical navigation. We present the simulation and actual image restoration experiment to demonstrate the accuracy and consistency of the relative position of the recovered navigation image of the proposed method. We show that the proposed method presents superior performance in comparison with the multiple cross correlation method. The estimated PSF is close to real PSF and the distributed energy of the beacon is concentrated ensuring a high SNR, where the accuracy of the relative position is higher than 0.1 pixel.

  20. Type of equipment in an air monitoring station

    International Nuclear Information System (INIS)

    Levy, F.

    1999-01-01

    This poster communication presents a general view of the station, the different materials used for the air and precipitations monitoring. The methods for gas measurements (tritium, krypton, iodine) are given. The collecting of dusts, dust and tritium mixed together, or rainwater and pluviometry are reviewed and the measurement of ambient radioactivity is made by beacon. (N.C.)

  1. "Off-on" electrochemical hairpin-DNA-based genosensor for cancer diagnostics.

    Science.gov (United States)

    Farjami, Elaheh; Clima, Lilia; Gothelf, Kurt; Ferapontova, Elena E

    2011-03-01

    A simple and robust "off-on" signaling genosensor platform with improved selectivity for single-nucleotide polymorphism (SNP) detection based on the electronic DNA hairpin molecular beacons has been developed. The DNA beacons were immobilized onto gold electrodes in their folded states through the alkanethiol linker at the 3'-end, while the 5'-end was labeled with a methylene blue (MB) redox probe. A typical "on-off" change of the electrochemical signal was observed upon hybridization of the 27-33 nucleotide (nt) long hairpin DNA to the target DNA, in agreement with all the hitherto published data. Truncation of the DNA hairpin beacons down to 20 nts provided improved genosensor selectivity for SNP and allowed switching of the electrochemical genosensor response from the on-off to the off-on mode. Switching was consistent with the variation in the mechanism of the electron transfer reaction between the electrode and the MB redox label, for the folded beacon being characteristic of the electrochemistry of adsorbed species, while for the "open" duplex structure being formally controlled by the diffusion of the redox label within the adsorbate layer. The relative current intensities of both processes were governed by the length of the formed DNA duplex, potential scan rate, and apparent diffusion coefficient of the redox species. The off-on genosensor design used for detection of a cancer biomarker TP53 gene sequence favored discrimination between the healthy and SNP-containing DNA sequences, which was particularly pronounced at short hybridization times.

  2. "The Mind Has to Catch Up on Sex": Sexual Norms and Sex Education in the Hull House

    Science.gov (United States)

    Fair, Alexandra

    2018-01-01

    From its beginning in 1885, the Hull House was beacon for social progress and urban reform. Founders Jane Addams and Ellen Gates Starr recruited talented, passionate partners from diverse fields to address issues from street sanitation to education in Chicago's immigrant communities. Among residents' many projects, their involvement in the…

  3. Evaluation of Congestion-based Certificate Omission in VANETs

    NARCIS (Netherlands)

    Feiri, Michael; Petit, Jonathan; Kargl, Frank

    2012-01-01

    Telematic awareness of nearby vehicles is a basic foundation of electronic safety applications in Vehicular Ad hoc Networks (VANETs). This awareness is achieved by frequently broadcasting beacon messages to nearby vehicles that announce a vehicle’s location and other data like heading and speed.

  4. Congestion-based Certificate Omission in VANETs

    NARCIS (Netherlands)

    Feiri, Michael; Petit, Jonathan; Kargl, Frank

    Telematic awareness of nearby vehicles is a basic foundation of electronic safety applications in Vehicular Ad hoc Networks (VANETs). This awareness is achieved by frequently broadcasting beacon messages to nearby vehicles that announce a vehicle's location and other data like heading and speed.

  5. Beyond categories, proper names, types and norms toward a fragile ...

    African Journals Online (AJOL)

    2012-01-16

    Jan 16, 2012 ... Ricoeur, P., 1976, Interpretation theory: Discourse & the surplus meaning, Texas. Christian University Press, Fort Worth, TX. Ricoeur, P., 1978, The philosophy of Paul Ricoeur: An anthology of his work, eds. C.E.. Regan & D. Stewart, Beacon Press, Boston, MA. Ricoeur, P., 1980, 'Toward a hermeneutic of ...

  6. An Approach to Analysing the Quality of Menu Translations in Southern Spain Restaurants

    Science.gov (United States)

    Fuentes-Luque, Adrián

    2017-01-01

    Cuisine and restaurants are powerful tools for cultural, social and tourist image-building, and projection for tourist promotion, particularly in the case of major tourist places and destinations which boast a well-deserved, long-standing history and reputation for gastronomical beacons. When menus are not properly translated (or transcreated)…

  7. 78 FR 48840 - Proposed Amendment of Class E Airspace; Hampton, IA

    Science.gov (United States)

    2013-08-12

    ...-0585; Airspace Docket No. 13-ACE-7] Proposed Amendment of Class E Airspace; Hampton, IA AGENCY: Federal... proposes to amend Class E airspace at Hampton, IA. Decommissioning of the Hampton non-directional beacon (NDB) at Hampton Municipal Airport has made reconfiguration necessary for standard instrument approach...

  8. A method for optical ground station reduce alignment error in satellite-ground quantum experiments

    Science.gov (United States)

    He, Dong; Wang, Qiang; Zhou, Jian-Wei; Song, Zhi-Jun; Zhong, Dai-Jun; Jiang, Yu; Liu, Wan-Sheng; Huang, Yong-Mei

    2018-03-01

    A satellite dedicated for quantum science experiments, has been developed and successfully launched from Jiuquan, China, on August 16, 2016. Two new optical ground stations (OGSs) were built to cooperate with the satellite to complete satellite-ground quantum experiments. OGS corrected its pointing direction by satellite trajectory error to coarse tracking system and uplink beacon sight, therefore fine tracking CCD and uplink beacon optical axis alignment accuracy was to ensure that beacon could cover the quantum satellite in all time when it passed the OGSs. Unfortunately, when we tested specifications of the OGSs, due to the coarse tracking optical system was commercial telescopes, the change of position of the target in the coarse CCD was up to 600μrad along with the change of elevation angle. In this paper, a method of reduce alignment error between beacon beam and fine tracking CCD is proposed. Firstly, OGS fitted the curve of target positions in coarse CCD along with the change of elevation angle. Secondly, OGS fitted the curve of hexapod secondary mirror positions along with the change of elevation angle. Thirdly, when tracking satellite, the fine tracking error unloaded on the real-time zero point position of coarse CCD which computed by the firstly calibration data. Simultaneously the positions of the hexapod secondary mirror were adjusted by the secondly calibration data. Finally the experiment result is proposed. Results show that the alignment error is less than 50μrad.

  9. A unique radiation area monitoring system

    International Nuclear Information System (INIS)

    Murphy, P.C.; Allen, G.C.

    1978-01-01

    The Remote Area Monitoring Systems (RAMS) monitors four radiation areas with two independent systems in each area. Each system consists of power supplies, four ionization chambers, and four analog and digital circuits. The first system controls the warning beacons, horns, annunciation panel and interlocks. The second system presents a quantitative dose rate indication at the console and in the radiation area

  10. Molecular detection and variability of Strawberry vein banding virus

    Czech Academy of Sciences Publication Activity Database

    Hanzlíková-Vašková, Dana; Špak, Josef

    2004-01-01

    Roč. 656, - (2004), 33-38 ISSN 0567-7572 Grant - others:EU EU QLR5-1999-1553 Institutional research plan: CEZ:AV0Z5051902 Keywords : AmpliDet RNA * caulimovirus * molecular beacon * nucleic acid sequence based amplification * PCR, SVBV * CaM Subject RIV: EE - Microbiology, Virology

  11. 78 FR 21043 - Amendment of Class D and Class E Airspace; Caldwell, NJ

    Science.gov (United States)

    2013-04-09

    ...-0609; Airspace Docket No. 12-AEA-10] Amendment of Class D and Class E Airspace; Caldwell, NJ AGENCY... Class E Airspace at Caldwell, NJ as the Paterson Non-Directional Radio Beacon (NDB) has been... D and Class E airspace at Caldwell, NJ (78 FR 5149) Docket No. FAA-2012-0609. Interested parties...

  12. Living history – the story of Adderley Street's fl ower sellers | Rabe ...

    African Journals Online (AJOL)

    Cape Town is probably synonymous with Table Mountain. But one of the colourful scenes at the foot of the mountain may also be described as synonymous with the city: Adderley Street's “famous” flower market. Yet, although the flower sellers are part of Cape Town's living history, a beacon for tourists, and literally ...

  13. 76 FR 30298 - Proposed Amendment of Class E Airspace; Cocoa, FL

    Science.gov (United States)

    2011-05-25

    ...-0070; Airspace Docket No. 10-ASO-43] Proposed Amendment of Class E Airspace; Cocoa, FL AGENCY: Federal... proposes to amend Class E Airspace at Cocoa, FL, as the Merritt Island Non-Directional Beacon (NDB) has... surface to support new standard instrument approach procedures developed at Merritt Island Airport, Cocoa...

  14. 75 FR 35951 - World Refugee Day, 2010

    Science.gov (United States)

    2010-06-23

    ... Refugee Day, 2010 By the President of the United States of America A Proclamation On World Refugee Day, we... refugees enrich our Nation and strengthen our unique narrative--that America stands as a beacon of hope and... and communities. This year marks the 30th anniversary of the Refugee Act of 1980. This historic...

  15. Privilege, shame and new choices towards reconciliation. An autobiographical approach

    Directory of Open Access Journals (Sweden)

    J C M�ller

    2005-10-01

    Full Text Available A narrative and autobiographical approach is taken in this article in order to explore and explain reconciliation. In the process the concepts of privilege, shame and new choices, within a specific story, are taken as the guiding beacons on the road to reconciliation in the South African situation.�

  16. 76 FR 1377 - Proposed Amendment of Class E Airspace; Mosby, MO

    Science.gov (United States)

    2011-01-10

    ... Federal Aviation Administration 14 CFR Part 71 Proposed Amendment of Class E Airspace; Mosby, MO AGENCY... action proposes to amend Class E airspace at Mosby, MO. Decommissioning of the Mosby non-directional beacon (NDB) at Midwest National Air Center Airport, Mosby, MO, has made this action necessary for the...

  17. 76 FR 31822 - Amendment of Class E Airspace; Mosby, MO

    Science.gov (United States)

    2011-06-02

    ... Federal Aviation Administration 14 CFR Part 71 Amendment of Class E Airspace; Mosby, MO AGENCY: Federal... Mosby, MO. Decommissioning of the Mosby non-directional beacon (NDB) at Midwest National Air Center Airport, Mosby, MO, has made this action necessary to enhance the safety and management of Instrument...

  18. 78 FR 68037 - Science Advisory Board (SAB)

    Science.gov (United States)

    2013-11-13

    ... (DOC). ACTION: Notice of time change and meeting location. SUMMARY: The notice of an open meeting of.... on November 20, 2013 to 11:30 a.m. The meeting will be held at the Beacon Hotel, 1615 Rhode Island... the most recent agenda and directions to the meeting location. FOR FURTHER INFORMATION CONTACT: Dr...

  19. Randomization and Data-Analysis Items in Quality Standards for Single-Case Experimental Studies

    Science.gov (United States)

    Heyvaert, Mieke; Wendt, Oliver; Van den Noortgate, Wim; Onghena, Patrick

    2015-01-01

    Reporting standards and critical appraisal tools serve as beacons for researchers, reviewers, and research consumers. Parallel to existing guidelines for researchers to report and evaluate group-comparison studies, single-case experimental (SCE) researchers are in need of guidelines for reporting and evaluating SCE studies. A systematic search was…

  20. 76 FR 21266 - Proposed Amendment of Class E Airspace; Cocoa, FL

    Science.gov (United States)

    2011-04-15

    ... Federal Aviation Administration 14 CFR Part 71 Proposed Amendment of Class E Airspace; Cocoa, FL AGENCY... action proposes to amend Class E Airspace at Cocoa, FL, as ] the Merritt Island Non-Directional Beacon... surface to support new standard instrument approach procedures developed at Merritt Island Airport, Cocoa...

  1. 2-Aminopurine hairpin probes for the detection of ultraviolet-induced DNA damage

    International Nuclear Information System (INIS)

    El-Yazbi, Amira F.; Loppnow, Glen R.

    2012-01-01

    Highlights: ► Molecular beacon with 2AP bases detects DNA damage in a simple mix-and-read assay. ► Molecular beacons with 2AP bases detect damage at a 17.2 nM limit of detection. ► The 2AP molecular beacon is linear over a 0–3.5 μM concentration range for damage. - Abstract: Nucleic acid exposure to radiation and chemical insults leads to damage and disease. Thus, detection and understanding DNA damage is important for elucidating molecular mechanisms of disease. However, current methods of DNA damage detection are either time-consuming, destroy the sample, or are too specific to be used for generic detection of damage. In this paper, we develop a fluorescence sensor of 2-aminopurine (2AP), a fluorescent analogue of adenine, incorporated in the loop of a hairpin probe for the quantification of ultraviolet (UV) C-induced nucleic acid damage. Our results show that the selectivity of the 2AP hairpin probe to UV-induced nucleic acid damage is comparable to molecular beacon (MB) probes of DNA damage. The calibration curve for the 2AP hairpin probe shows good linearity (R 2 = 0.98) with a limit of detection of 17.2 nM. This probe is a simple, fast and economic fluorescence sensor for the quantification of UV-induced damage in DNA.

  2. Making Science Meaningful for Young People with Diverse Needs

    Science.gov (United States)

    Stead, Di

    2015-01-01

    In the autumn term of 2011, it was identified that a programme of support for developing science in the curriculum was required at a newly opened special school, Beacon Reach, in Lancashire, which caters to children with autism spectrum disorders. Staff wanted to provide a relevant and meaningful curriculum, accessible even to young people who…

  3. Eboo Patel (2012). Sacred Ground: Pluralism, Prejudice and the ...

    African Journals Online (AJOL)

    Sacred Ground: Pluralism, Prejudice and the Promise of America. Boston: Beacon Press. Reviewed by Jon C. Dalton*. In his 2012 book, Sacred Ground, Eboo Patel, an American Muslim and President of the Interfaith Student Core in Chicago, Illinois, analyses the sources of contemporary interreligious conflict and offers ...

  4. An Efficient and Self-Adapting Localization in Static Wireless Sensor Networks

    Directory of Open Access Journals (Sweden)

    Wei Dong

    2009-08-01

    Full Text Available Localization is one of the most important subjects in Wireless Sensor Networks (WSNs. To reduce the number of beacons and adopt probabilistic methods, some particle filter-based mobile beacon-assisted localization approaches have been proposed, such as Mobile Beacon-assisted Localization (MBL, Adapting MBL (A-MBL, and the method proposed by Hang et al. Some new significant problems arise in these approaches, however. The first question is which probability distribution should be selected as the dynamic model in the prediction stage. The second is whether the unknown node adopts neighbors’ observation in the update stage. The third is how to find a self-adapting mechanism to achieve more flexibility in the adapting stage. In this paper, we give the theoretical analysis and experimental evaluations to suggest which probability distribution in the dynamic model should be adopted to improve the efficiency in the prediction stage. We also give the condition for whether the unknown node should use the observations from its neighbors to improve the accuracy. Finally, we propose a Self-Adapting Mobile Beacon-assisted Localization (SA-MBL approach to achieve more flexibility and achieve almost the same performance with A-MBL.

  5. 76 FR 73502 - Amendment of Class E Airspace; Ardmore, OK

    Science.gov (United States)

    2011-11-29

    ... Federal Aviation Administration 14 CFR Part 71 Amendment of Class E Airspace; Ardmore, OK AGENCY: Federal... Ardmore, OK. Decommissioning of the Arbuckle non-directional beacon (NDB) and cancellation of the NDB Standard Instrument Approach Procedure (SIAP) at Ardmore Municipal Airport, Ardmore, OK, as well as the...

  6. 76 FR 75446 - Amendment of Class E Airspace; Mercury, NV

    Science.gov (United States)

    2011-12-02

    ...-0894; Airspace Docket No. 11-AWP-14] Amendment of Class E Airspace; Mercury, NV AGENCY: Federal... Mercury, Desert Rock Airport, Mercury, NV. Decommissioning of the Mercury Non-Directional Beacon (NDB) at Mercury, Desert Rock Airport has made this action necessary for the safety and management of Instrument...

  7. 76 FR 56127 - Proposed Amendment of Class E Airspace; Mercury, NV

    Science.gov (United States)

    2011-09-12

    ...-0894; Airspace Docket No. 11-AWP-14] Proposed Amendment of Class E Airspace; Mercury, NV AGENCY... action proposes to amend Class E airspace at Mercury, Desert Rock Airport, Mercury, NV. Decommissioning of the Mercury Non- Directional Beacon (NDB) at Mercury, Desert Rock Airport has made this action...

  8. 77 FR 41709 - Common Crop Insurance Regulations; Florida Citrus Fruit Crop Insurance Provisions

    Science.gov (United States)

    2012-07-16

    ... type, intended use, and age of trees within a citrus fruit commodity, times the coverage level percent... changes, to clarify existing policy provisions to better meet the needs of policyholders, and to reduce... Administration and Standards Division, Risk Management Agency, United States Department of Agriculture, Beacon...

  9. 78 FR 46249 - Common Crop Insurance Regulations; Arizona-California Citrus Crop Insurance Provisions

    Science.gov (United States)

    2013-07-31

    ... purposes of electing coverage levels and identifying the insured crop. Commodity type. A specific subgroup... Provisions. The intended effect of this action is to provide policy changes and clarify existing policy... and Standards Division, Risk Management Agency, United States Department of Agriculture, Beacon...

  10. 27 CFR 4.91 - List of approved prime names.

    Science.gov (United States)

    2010-04-01

    ... blanc Baco noir Barbera Beacon Beclan Bellandais Beta Black Corinth Black Pearl Blanc Du Bois Blue Eye... blanche Clinton Colombard (French Colombard) Colobel Cortese Corvina Concord Conquistador Couderc noir... Fredonia Freisa Fry Furmint Gamay noir Garronet Gewürztraminer Gladwin 113 Glennel Gold Golden Isles Golden...

  11. Show me the way: proximity layered feedback services in smart cities

    NARCIS (Netherlands)

    Tabuenca, Bernardo; Börner, Dirk; Kalz, Marco; Specht, Marcus

    2014-01-01

    The advent of Bluetooth Low Energy (Bluetooth LE) technology and its native implementation within the main smartphone manufacturers is ac- celerating the integration of these sensors in smart cities. Bluetooth LE beacons are being novelty used to provide proximity-adapted feedback in the field of

  12. Show me the way: proximity layered feedback services in smart cities

    NARCIS (Netherlands)

    Tabuenca, Bernardo

    2014-01-01

    The advent of Bluetooth Low Energy (Bluetooth LE) technology and its native implementation within the main smartphone manufacturers is accelerating the integration of these sensors in smart cities. Bluetooth LE beacons are being novelty used to provide proximity-adapted feedback in the field of

  13. Estimating orientation with gyroscopes and accelerometers

    NARCIS (Netherlands)

    Luinge, Hendrik J.; Veltink, Petrus H.; Baten, Christian T.M.

    1999-01-01

    Many systems for recording human movement need some reference from beacons near the subject, such as video cameras. Our goal is to measure human kinematics with sensors that are placed on the segments of interest. This way, experiments in which human movement is recorded are not restricted to a lab.

  14. 14 CFR 171.213 - Reports.

    Science.gov (United States)

    2010-01-01

    ... 14 Aeronautics and Space 3 2010-01-01 2010-01-01 false Reports. 171.213 Section 171.213... FACILITIES NON-FEDERAL NAVIGATION FACILITIES VHF Marker Beacons § 171.213 Reports. The owner of each facility to which this subpart applies shall make the following reports, at the times indicated, to the...

  15. 14 CFR 171.33 - Reports.

    Science.gov (United States)

    2010-01-01

    ... 14 Aeronautics and Space 3 2010-01-01 2010-01-01 false Reports. 171.33 Section 171.33 Aeronautics... FACILITIES NON-FEDERAL NAVIGATION FACILITIES Nondirectional Radio Beacon Facilities § 171.33 Reports. The owner of each facility to which this subpart applies shall make the following reports, at the times...

  16. 78 FR 52832 - Catastrophic Risk Protection Endorsement

    Science.gov (United States)

    2013-08-27

    ... Management Agency, United States Department of Agriculture, Beacon Facility, Stop 0812, Room 421, P.O. Box... 12866 The Office of Management and Budget (OMB) has determined that this rule is not-significant for the... is definite, and whether ACRSI will be implemented before the Catastrophic Risk Protection...

  17. Reflections

    Indian Academy of Sciences (India)

    With their vision and foresight, these two great scientists realized the importance of this new and expanding field of radio physics and electronics ..... electronics, magnetron fabrication, and ionospheric investigations from artificial satellite beacon studies were carried out with significant achievements, which were duly recog-.

  18. Real-Time Signal Processor for Pulsar Studies P. S. Ramkumar

    Indian Academy of Sciences (India)

    R. Narasimhan (Krishtel eMaging) 1461 1996 Oct 15 13:05:22

    odically, much like a rotating beacon, resulting in a periodic train of narrow pulses of broad-band radiation, which can be detected ... Pulsar signals appear as a train of highly periodic pulses, with period typically in the range of a ..... An interrupt service routine in the DSP routine gets activated immediately and removes the ...

  19. The young and the digital, by S. Craig Watkins [book review

    Directory of Open Access Journals (Sweden)

    Melanie E. S. Kohnen

    2011-11-01

    Full Text Available Review of S. Craig Watkins, The young and the digital: What migration to social-networking sites, games, and anytime, anywhere media means for our future. Boston: Beacon Press, 2009, paperback, $18 (272p ISBN 978-0807006160.

  20. One-trial spatial learning: wild hummingbirds relocate a reward after a single visit.

    Science.gov (United States)

    Flores-Abreu, I Nuri; Hurly, T Andrew; Healy, Susan D

    2012-07-01

    Beaconing to rewarded locations is typically achieved by visual recognition of the actual goal. Spatial recognition, on the other hand, can occur in the absence of the goal itself, relying instead on the landmarks surrounding the goal location. Although the duration or frequency of experiences that an animal needs to learn the landmarks surrounding a goal have been extensively studied with a variety of laboratory tasks, little is known about the way in which wild vertebrates use them in their natural environment. Here, we allowed hummingbirds to feed once only from a rewarding flower (goal) before it was removed. When we presented a similar flower at a different height in another location, birds frequently returned to the location the flower had previously occupied (spatial recognition) before flying to the flower itself (beaconing). After experiencing three rewarded flowers, each in a different location, they were more likely to beacon to the current visible flower than they were to return to previously rewarded locations (without a visible flower). These data show that hummingbirds can encode a rewarded location on the basis of the surrounding landmarks after a single visit. After multiple goal location manipulations, however, the birds changed their strategy to beaconing presumably because they had learned that the flower itself reliably signalled reward.

  1. Short Comm.

    African Journals Online (AJOL)

    E-LIBRARY

    Practical role of library and information science professionals towards sustainable industrialization in Nigeria. Entrepreneurship: Trained librarians no longer belong to the class of the oppressed or rather the victims; librarians now are beacons of hope, as small scale enterprises, companies, state of art construction, firms'.

  2. U.S. COIN Doctrine: Betting the Future on a Too Distant Past

    Science.gov (United States)

    2012-05-17

    Lawrence’s often quoted expression that compared insurgency to eating soup with a knife still resonates today, his insurgency experience related more...could 60 Dan Ellsberg and other unknown recipients , by Albert Wohlstetter, August 30, 1968...Beacon Press, 1966. Nagl, John A. Learning to Eat Soup with a Knife: Counterinsurgency Lessons from Malaya and Vietnam. Paperback ed. Chicago

  3. Some Thoughts (and Afterthoughts) on Context, Interpretation, and Organization Theory.

    Science.gov (United States)

    1982-02-01

    Habermas (especially 1971, 1975) is perhaps the classic current source, Marcuse (1964) the most widely read. Jay (1973) provides a readable and thorough...driving and the symbolic order. Chicago: University of Chicago Press, 1980. Habermas ,J. Knowledge and human interests. Boston: Beacon Press, 1971

  4. A transportable hybrid antenna-transmitter system for the generation of elliptically polarized waves for NVIS propagation research

    NARCIS (Netherlands)

    Witvliet, Ben A.; Laanstra, Geert J.; van Maanen, Erik; Alsina-Pagès, Rosa M.; Bentum, Marinus Jan; Slump, Cornelis H.; Schiphorst, Roelof

    2016-01-01

    For empirical research on Near Vertical Incidence Skywave (NVIS) characteristic wave propagation, a beacon transmitter system is needed that can be programmed to emit precisely defined elliptically and circularly polarized waves at high elevation angles. This paper proposes a novel hybrid

  5. Some aspects of the Olympus propagation experiment carried out by the Research Institute of the Deutsche Bundespost TELEKOM

    Science.gov (United States)

    Dintelmann, F.; Ortgies, G.; Ruecker, F.

    The Olympus propagation experiment carried out by the Research Institute of the Deutsche Bundespost Telekom is discussed. The objectives were to study cloud and rain attenuation and depolarization and scintillation effects. Olympus beacon terminal and data-acquisition and editing software are described.

  6. Per-Pixel, Dual-Counter Scheme for Optical Communications

    Science.gov (United States)

    Farr, William H.; Bimbaum, Kevin M.; Quirk, Kevin J.; Sburlan, Suzana; Sahasrabudhe, Adit

    2013-01-01

    Free space optical communications links from deep space are projected to fulfill future NASA communication requirements for 2020 and beyond. Accurate laser-beam pointing is required to achieve high data rates at low power levels.This innovation is a per-pixel processing scheme using a pair of three-state digital counters to implement acquisition and tracking of a dim laser beacon transmitted from Earth for pointing control of an interplanetary optical communications system using a focal plane array of single sensitive detectors. It shows how to implement dim beacon acquisition and tracking for an interplanetary optical transceiver with a method that is suitable for both achieving theoretical performance, as well as supporting additional functions of high data rate forward links and precision spacecraft ranging.

  7. Radio/FADS/IMU integrated navigation for Mars entry

    Science.gov (United States)

    Jiang, Xiuqiang; Li, Shuang; Huang, Xiangyu

    2018-03-01

    Supposing future orbiting and landing collaborative exploration mission as the potential project background, this paper addresses the issue of Mars entry integrated navigation using radio beacon, flush air data sensing system (FADS), and inertial measurement unit (IMU). The range and Doppler information sensed from an orbiting radio beacon, the dynamic pressure and heating data sensed from flush air data sensing system, and acceleration and attitude angular rate outputs from an inertial measurement unit are integrated in an unscented Kalman filter to perform state estimation and suppress the system and measurement noise. Computer simulations show that the proposed integrated navigation scheme can enhance the navigation accuracy, which enables precise entry guidance for the given Mars orbiting and landing collaborative exploration mission.

  8. A Tree Based Self-routing Scheme for Mobility Support in Wireless Sensor Networks

    Science.gov (United States)

    Kim, Young-Duk; Yang, Yeon-Mo; Kang, Won-Seok; Kim, Jin-Wook; An, Jinung

    Recently, WSNs (Wireless Sensor Networks) with mobile robot is a growing technology that offer efficient communication services for anytime and anywhere applications. However, the tiny sensor node has very limited network resources due to its low battery power, low data rate, node mobility, and channel interference constraint between neighbors. Thus, in this paper, we proposed a tree based self-routing protocol for autonomous mobile robots based on beacon mode and implemented in real test-bed environments. The proposed scheme offers beacon based real-time scheduling for reliable association process between parent and child nodes. In addition, it supports smooth handover procedure by reducing flooding overhead of control packets. Throughout the performance evaluation by using a real test-bed system and simulation, we illustrate that our proposed scheme demonstrates promising performance for wireless sensor networks with mobile robots.

  9. Independent effects of geometry and landmark in a spontaneous reorientation task: a study of two species of fish.

    Science.gov (United States)

    Lee, Sang Ah; Vallortigara, Giorgio; Ruga, Vincenza; Sovrano, Valeria A

    2012-09-01

    While disoriented humans and animals use both landmarks and environmental geometry to guide their navigation, it is not clear what kinds of cognitive mechanisms underlie these behaviors. Because traditional tests of trained navigation behavior in environments containing both landmarks and geometric information may cloud our insight into the nature of these processes, the present study tested the spontaneous use of landmarks and environmental shape by two species of fish-Redtail Splitfins (Xenotoca eiseni) and Zebrafish (Danio rerio). The results suggest that while geometry is spontaneously used by both species and both sexes to compute relative position or direction, the spontaneous use of landmarks is limited to direct beaconing and complicated by attraction to features and variability across species and sex. These findings support the view that while multiple cues may ultimately guide behavior, the computation of orientation and relative positions is specified by geometric input and is independent from other navigation processes such as beaconing.

  10. Research on Improved DV-HOP Algorithm against Wormhole Attacks in WSN

    Directory of Open Access Journals (Sweden)

    Wang Xue-Wen

    2016-01-01

    Full Text Available The secure location of node is significant in the WSN (Wireless Sensor Networks of the troop frontier defence system. The wormhole attack is a big threat in the secure location. The credibility of the beacon node was used to determine the malicious nodes produced by wormhole attack in the WSN. The estimated method of multibeacon nodes was adopted to improve DV-HOP algorithm after excluding the malicious nodes. In this paper, we compared the basic DV-HOP algorithm and the improved DV-HOP algorithm in the coverage percentage and the error of network localization by simulating. The simulation results indicate that the improved DV-HOP algorithm makes the localization coverage percentage can reach 90% on a certain scale of the network, and it makes the error percentage lower when the number of beacons is different.

  11. Gold Nanoparticle-Based Sensors Activated by External Radio Frequency Fields

    DEFF Research Database (Denmark)

    Della Vedova, Paolo; Ilieva, Mirolyuba; Zhurbenko, Vitaliy

    2015-01-01

    A novel molecular beacon (a nanomachine) is constructed that can be actuated by a radio frequency (RF) field. The nanomachine consists of the following elements arranged in molecular beacon configuration: a gold nanoparticle that acts both as quencher for fluorescence and a localized heat source...... by substituting the gold nanoparticle by an organic quencher, shows no increase in fluorescence signal when exposed to the RF field. It may therefore be concluded that the increased fluorescence for the gold nanoparticleconjugated nanomachines is not due to bulk heating of the solution, but is caused...... by the presence of the gold nanoparticles and their interaction with the RF field; however, existing models for heating of gold nanoparticles in a RF field are unable to explain the experimental results. Due to the biocompatibility of the construct and RF treatment, the nanomachines may possibly be used inside...

  12. Development and flight test of a helicopter compact, portable, precision landing system concept

    Science.gov (United States)

    Bull, J. S.; Clary, G. R.; Davis, T. J.; Chisholm, J. P.

    1984-01-01

    An airborne, radar based, precision approach concept is being developed and flight tested as a part of NASA's Rotorcraft All-Weather Operations Research Program. A transponder based beacon landing system (BLS) applying state of the art X band radar technology and digital processing techniques, has been built and is being flight tested to demonstrate the concept feasibility. The BLS airborne hardware consists of an add on microprocessor, installed in conjunction with the aircraft weather/mapping radar, which analyzes the radar beacon receiver returns and determines range, localizer deviation, and glide slope derivation. The ground station is an inexpensive, portable unit which can be quickly deployed at a landing site. Results from the flight test program show that the BLS concept has a significant potential for providing rotorcraft with low cost, precision instrument approach capability in remote areas.

  13. A novel generalized design methodology and realization of Boolean operations using DNA.

    Science.gov (United States)

    Zoraida, B S E; Arock, Michael; Ronald, B S M; Ponalagusamy, R

    2009-09-01

    The biological deoxyribonucleic acid (DNA) strand has been increasingly seen as a promising computing unit. A new algorithm is formulated in this paper to design any DNA Boolean operator with molecular beacons (MBs) as its input. Boolean operators realized using the proposed design methodology is presented. The developed operators adopt a uniform representation for logical 0 and 1 for any Boolean operator. The Boolean operators designed in this work employ only a hybridization operation at each stage. Further, this paper for the first time brings out the realization of a binary adder and subtractor using molecular beacons. Simulation results of the DNA-based binary adder and subtractor are given to validate the design.

  14. Self-aligning LED-based optical link

    Science.gov (United States)

    Shen, Thomas C.; Drost, Robert J.; Rzasa, John R.; Sadler, Brian M.; Davis, Christopher C.

    2016-09-01

    The steady advances in light-emitting diode (LED) technology have motivated the use of LEDs in optical wireless communication (OWC) applications such as indoor local area networks (LANs) and communication between mobile platforms (e.g., robots, vehicles). In contrast to traditional radio frequency (RF) wireless communication, OWC utilizes electromagnetic spectrum that is largely unregulated and unrestricted. OWC communication may be especially useful in RF-denied environments, in which RF communication may be prohibited or undesirable. However, OWC does present some challenges, including the need to maintain alignment between potentially moving nodes. We describe a novel system for link alignment that is composed of a hyperboloidal mirror, camera, and gimbal. The experimental system is able to use the mirror and camera to detect an LED beacon of a neighboring node and estimate its bearing (azimuth and elevation), point the gimbal towards the beacon, and establish an optical link.

  15. An iconic position estimator for a 2-D laser rangefinder

    Science.gov (United States)

    Gonzalez, Javier; Stentz, Anthony; Ollero, Anibal

    1992-05-01

    Position determination for a mobile robot is an important part of autonomous navigation. In many cases, dead reckoning is insufficient because it leads to large inaccuracies over time. Beacon- and landmark-based estimators require the emplacement of beacons and the presence of natural or man-made structure respectively in the environment. In this paper we present a new algorithm for efficiently computing accurate position estimates based on a radially-scanning laser rangefinder that requires minimal structure in the environment. The algorithm employs a connected set of short lie segments to approximate the shape of any environment and can easily be constructed by the rangefinder itself. We describe techniques for efficiently managing the environment map, matching the sensor data to the map, and computing the robot's position. We present accuracy and runtime results for our implementation.

  16. Real-time polymerase chain reaction quantification of the transgenes for roundup ready corn and roundup ready soybean in soil samples.

    Science.gov (United States)

    Lerat, S; England, L S; Vincent, M L; Pauls, K P; Swanton, C J; Klironomos, J N; Trevors, J T

    2005-03-09

    A method for quantification of recombinant DNA for Roundup Ready (RR) corn and RR soybean in soil samples is described. Soil DNA from experimental field samples was extracted using a soil DNA extraction kit with a modified protocol. For the detection and quantification of recombinant DNA of RR corn and RR soybean, a molecular beacon and two pairs of specific primers were designed to differentially target recombinant DNA in these two genetically modified crops. Soil DNA extracts were spiked with RR corn or RR soybean DNA, and recombinant DNA was quantified using real-time PCR with a molecular beacon. As few as one copy of RR corn genome or one copy of RR soybean genome was detected in the soil DNA extract.

  17. Costs and Difficulties of Interstellar 'Messaging' and the Need for International Debate on Potential Risks

    Science.gov (United States)

    Billingham, J.; Benford, James

    We advocate international consultations on societal and technical issues to address the risk of Messaging to Extraterrestrial Intelligence (METI) transmissions, and a moratorium on future transmissions until such issues are resolved. Instead, we recommend continuing to conduct SETI by listening, with no innate risk, while using powerful new search systems to give a better total probability of detection of beacons and messages than METI for the same cost, and with no need for a long obligatory wait for a response. Realistically, beacons are costly. In light of recent work on the economics of contact by radio, we offer alternatives to the current standard methods of SETI searches. METI transmissions to date are faint and very unlikely to be detected, even by nearby stars. We show that historical leakage from Earth has been undetectable for Earth-scale receiver systems. Future space microwave and laser power systems will likely be more detectable.

  18. Analysis of short-term reactor cavity transient

    International Nuclear Information System (INIS)

    Cheng, T.C.; Fischer, S.R.

    1981-01-01

    Following the transient of a hypothetical loss-of-coolant accident (LOCA) in a nuclear reactor, peak pressures are reached within the first 0.03 s at different locations inside the reactor cavity. Due to the complicated multidimensional nature of the reactor cavity, the short-term analysis of the LOCA transient cannot be performed by using traditional containment codes, such as CONTEMPT. The advanced containment code, BEACON/MOD3, developed at the Idaho National Engineering Laboratory (INEL), can be adapted for such analysis. This code provides Eulerian, one and two-dimensional, nonhomogeneous, nonequilibrium flow modeling as well as lumped parameter, homogeneous, equilibrium flow modeling for the solution of two-component, two-phase flow problems. The purpose of this paper is to demonstrate the capability of the BEACON code to analyze complex containment geometry such as a reactor cavity

  19. Note: Methodology for the analysis of Bluetooth gateways in an implemented scatternet.

    Science.gov (United States)

    Etxaniz, J; Monje, P M; Aranguren, G

    2014-03-01

    This Note introduces a novel methodology to analyze the time performance of Bluetooth gateways in multi-hop networks, known as scatternets. The methodology is focused on distinguishing between the processing time and the time that each communication between nodes takes along an implemented scatternet. This technique is not only valid for Bluetooth networks but also for other wireless networks that offer access to their middleware in order to include beacons in the operation of the nodes. We show in this Note the results of the tests carried out on a Bluetooth scatternet in order to highlight the reliability and effectiveness of the methodology. The results also validate this technique showing convergence in the results when subtracting the time for the beacons from the delay measurements.

  20. Sun, Sand and Water: A History of the Jacksonville District U.S. Army Corps of Engineers 1821-1975

    Science.gov (United States)

    1981-01-01

    topographical engineers (Captains [John] MacKay and [Walter B.] Guion , Lieutenants [Jacob E.] Blake, [John W.] Gunnison, and [Robert M.] McLane...the Jacksonville District in December 1940. As was usual with air bases, auxiliary installations grew up around the field proper. A radio beacon and...Sound was one such base. This station was a joint training school for Signal Corps and Coast Artillery personnel learning to maintain radio detection

  1. Virtual acoustics, aeronautics, and communications.

    Science.gov (United States)

    Begault, D R

    1998-06-01

    An optimal approach to auditory display design for commercial aircraft would utilize both spatialized (3-D) audio techniques and active noise cancellation for safer operations. Results from several aircraft simulator studies conducted at NASA Ames Research Center are reviewed, including Traffic alert and Collision Avoidance System (TCAS) warnings, spoken orientation "beacons" for gate identification and collision avoidance on the ground, and hardware for improved speech intelligibility. The implications of hearing loss among pilots is also considered.

  2. Fort Jackson, a Preventive Approach to Family Life Ministry in a Military Community.

    Science.gov (United States)

    1979-12-01

    Nashville: Broadman Press, 1962), p. 256. 2W. T. Purkiser, The Gifts of the Spirit (Kansas City: Beacon Hill Press, 1975), p. 21. 24 principles. At...chaplaincy setting and geared to the needs of military couples. 1David Mace and Vera Mace, Marriage Enrichment in the Church (Nashville: Broadman ...Enrichment in the Church. Nashville: Broadman Press, 1976. Mace, David, and Mace, Vera. We Can Have Better Marriages. Nashville: Abingdon Press, 1974. Mack

  3. NPS-SCAT; Communications System Design, Test and Integration of NPS’ First CubeSat

    Science.gov (United States)

    2010-09-01

    changing ground station software configurations, the beacon would not respond to the commands. A second receiver ( ICOM PCR1500), a sniffer, was setup...45, is an ICOM IC-910H with two operating modes, one which is in the 70 cm amateur band 420 to 480 MHz. This frequency range is perfect for the...because this is what sends the data to the ICOM radio, which transmits to the spacecraft. The receiving settings are similar to the transmit

  4. Measurement of the group velocity of light in sea water at the ANTARES site

    Science.gov (United States)

    Adrián-Martínez, S.; Al Samarai, I.; Albert, A.; André, M.; Anghinolfi, M.; Anton, G.; Anvar, S.; Ardid, M.; Assis Jesus, A. C.; Astraatmadja, T.; Aubert, J.-J.; Baret, B.; Basa, S.; Bertin, V.; Biagi, S.; Bigi, A.; Bigongiari, C.; Bogazzi, C.; Bou-Cabo, M.; Bouhou, B.; Bouwhuis, M. C.; Brunner, J.; Busto, J.; Camarena, F.; Capone, A.; Cârloganu, C.; Carminati, G.; Carr, J.; Cecchini, S.; Charif, Z.; Charvis, Ph.; Chiarusi, T.; Circella, M.; Costantini, H.; Coyle, P.; Curtil, C.; De Bonis, G.; Decowski, M. P.; Dekeyser, I.; Deschamps, A.; Distefano, C.; Donzaud, C.; Dornic, D.; Dorosti, Q.; Drouhin, D.; Eberl, T.; Emanuele, U.; Enzenhöfer, A.; Ernenwein, J.-P.; Escoffier, S.; Fermani, P.; Ferri, M.; Flaminio, V.; Folger, F.; Fritsch, U.; Fuda, J.-L.; Galatà, S.; Gay, P.; Geyer, K.; Giacomelli, G.; Giordano, V.; Gómez-González, J. P.; Graf, K.; Guillard, G.; Halladjian, G.; Hallewell, G.; van Haren, H.; Hartman, J.; Heijboer, A. J.; Hello, Y.; Hernández-Rey, J. J.; Herold, B.; Hößl, J.; Hsu, C. C.; de Jong, M.; Kadler, M.; Kalekin, O.; Kappes, A.; Katz, U.; Kavatsyuk, O.; Kooijman, P.; Kopper, C.; Kouchner, A.; Kreykenbohm, I.; Kulikovskiy, V.; Lahmann, R.; Lamare, P.; Larosa, G.; Lattuada, D.; Lefèvre, D.; Lim, G.; Lo Presti, D.; Loehner, H.; Loucatos, S.; Mangano, S.; Marcelin, M.; Margiotta, A.; Martínez-Mora, J. A.; McMillan, J. E.; Meli, A.; Montaruli, T.; Moscoso, L.; Motz, H.; Neff, M.; Nezri, E.; Palioselitis, D.; Păvălaş, G. E.; Payet, K.; Payre, P.; Petrovic, J.; Piattelli, P.; Picot-Clemente, N.; Popa, V.; Pradier, T.; Presani, E.; Racca, C.; Reed, C.; Riccobene, G.; Richardt, C.; Richter, R.; Rivière, C.; Robert, A.; Roensch, K.; Rostovtsev, A.; Ruiz-Rivas, J.; Rujoiu, M.; Russo, G. V.; Salesa, F.; Samtleben, D. F. E.; Sapienza, P.; Schöck, F.; Schuller, J.-P.; Schüssler, F.; Seitz, T.; Shanidze, R.; Simeone, F.; Spies, A.; Spurio, M.; Steijger, J. J. M.; Stolarczyk, Th.; Sánchez-Losa, A.; Taiuti, M.; Tamburini, C.; Thompson, L. F.; Toscano, S.; Vallage, B.; Van Elewyck, V.; Vannoni, G.; Vecchi, M.; Vernin, P.; Wagner, S.; Wijnker, G.; Wilms, J.; de Wolf, E.; Yepes, H.; Zaborov, D.; Zornoza, J. D.; Zúñiga, J.

    2012-04-01

    The group velocity of light has been measured at eight different wavelengths between 385 nm and 532 nm in the Mediterranean Sea at a depth of about 2.2 km with the ANTARES optical beacon systems. A parametrisation of the dependence of the refractive index on wavelength based on the salinity, pressure and temperature of the sea water at the ANTARES site is in good agreement with these measurements.

  5. SU-G-JeP1-01: A Combination of Real Time Electromagnetic Localization and Tracking with Cone Beam Computed Tomography in Stereotactic Radiosurgery for Brain Tumors

    Energy Technology Data Exchange (ETDEWEB)

    Muralidhar, K Raja; Pangam, Suresh; Ponaganti, Srinivas; Krishna, Jayarama; Sujana, Kolla V; Komanduri, Priya K [American Oncology Institute, Hyderabad, Telangana (India)

    2016-06-15

    Purpose: 1. online verification of patient position during treatment using calypso electromagnetic localization and tracking system. 2. Verification and comparison of positional accuracy between cone beam computed tomography and calypso system. 3. Presenting the advantage of continuation localization in Stereotactic radiosurgery treatments. Methods: Ten brain tumor cases were taken for this study. Patients with head mask were under gone Computed Tomography (CT). Before scanning, mask was cut on the fore head area to keep surface beacons on the skin. Slice thickness of 0.65 mm were taken for this study. x, y, z coordinates of these beacons in TPS were entered into tracking station. Varian True Beam accelerator, equipped with On Board Imager was used to take Cone beam Computed Tomography (CBCT) to localize the patient. Simultaneously Surface beacons were used to localize and track the patient throughout the treatment. The localization values were compared in both systems. For localization CBCT considered as reference. Tracking was done throughout the treatment using Calypso tracking system using electromagnetic array. This array was in tracking position during imaging and treatment. Flattening Filter free beams of 6MV photons along with Volumetric Modulated Arc Therapy was used for the treatment. The patient movement was observed throughout the treatment ranging from 2 min to 4 min. Results: The average variation observed between calypso system and CBCT localization was less than 0.5 mm. These variations were due to manual errors while keeping beacon on the patient. Less than 0.05 cm intra-fraction motion was observed throughout the treatment with the help of continuous tracking. Conclusion: Calypso target localization system is one of the finest tools to perform radiosurgery in combination with CBCT. This non radiographic method of tracking is a real beneficial method to treat patients confidently while observing real-time motion information of the patient.

  6. Mobile Communications within the Deepwater Lifelines

    Science.gov (United States)

    2013-12-13

    Baumann, Ph.D. The opinions and conclusions expressed herein are those of the student author and do not necessarily represent the views of the U.S...internet cafes , Wi-Fi hotspots in public spaces, and high quality cellphone transmission to serve most passenger needs. On the other hand, internet...systems that are outdoor- based-only. Apple Corporation offers a service to clients know as iBeacon that offers very similar capabilities. Students

  7. A Shuttle based laser system for space communication

    Science.gov (United States)

    Fitzmaurice, Michael W.; Bruno, Ronald C.

    1988-01-01

    A key element of NASA-Goddard's plan for future laser space communications is the Space Shuttle-based Laser Technology Experiments Facility (LTEF), which will be designed to communicate with a cooperative laser system under development for the Advanced Communication Technology Satellite (ACTS) and will conduct a comprehensive set of acquisition, tracking, and communication experiments. Attention is presently given to the challenges faced by designers in achieving LTEF acquisition of the ACTS downlink beacon laser.

  8. Gold nanoparticle-based sensors activated by external radio frequency fields.

    Science.gov (United States)

    Vedova, Paolo Della; Ilieva, Mirolyuba; Zhurbenko, Vitaliy; Mateiu, Ramona; Faralli, Adele; Dufva, Martin; Hansen, Ole

    2015-01-14

    A novel molecular beacon (a nanomachine) is constructed that can be actuated by a radio frequency (RF) field. The nanomachine consists of the following elements arranged in molecular beacon configuration: a gold nanoparticle that acts both as quencher for fluorescence and a localized heat source; one reporter fluorochrome, and; a piece of DNA as a hinge and recognition sequence. When the nanomachines are irradiated with a 3 GHz RF field the fluorescence signal increases due to melting of the stem of the molecular beacon. A control experiment, performed using molecular beacons synthesized by substituting the gold nanoparticle by an organic quencher, shows no increase in fluorescence signal when exposed to the RF field. It may therefore be concluded that the increased fluorescence for the gold nanoparticle-conjugated nanomachines is not due to bulk heating of the solution, but is caused by the presence of the gold nanoparticles and their interaction with the RF field; however, existing models for heating of gold nanoparticles in a RF field are unable to explain the experimental results. Due to the biocompatibility of the construct and RF treatment, the nanomachines may possibly be used inside living cells. In a separate experiment a substantial increase in the dielectric losses can be detected in a RF waveguide setup coupled to a microfluidic channel when gold nanoparticles are added to a low RF loss liquid. This work sheds some light on RF heating of gold nanoparticles, which is a subject of significant controversy in the literature. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  9. Guide to Canadian Aerospace-Related Industries

    Science.gov (United States)

    1990-08-01

    serices t, hammers, stretch forming machines, lathes, millers, planers , jig borers government and ii dubti y ii Iritegiat ed Loystic ,S.,upp.rt (ILS...Systems, Aircraft, Aircraft Control, Airframe planers , multi-spindle router ,, spot and heli-arc welders, vertical and Components, Airframe Structures...National Defence - Heav , Logistics Vehicle KEYWORDS. Communications Towers, Beacon Antennas, HF Antennas, Wheeled System ($250M Cdn), Life Skill- _Jucation

  10. Equatorial Ionospheric Irregularities Study from ROCSAT Data

    Science.gov (United States)

    2017-10-20

    Earthquake . Signals in a time-series are not always stationary or linear. The best way to analyze such data is, to our best understanding, using the...intrinsic mode function) each has a perfect Hilbert transform and exhibits distinctive oscillation frequencies . Using the HHT method to analyze...the radio beacon data, we can find some hidden oscillations at some particular frequency in the signals that is not related to the density

  11. Fluorescence reporters for Hfq oligomerization and RNA annealing

    OpenAIRE

    Panja, Subrata; Woodson, Sarah A.

    2015-01-01

    Fluorescence spectroscopy is a sensitive technique for detecting protein-protein, protein-RNA and RNA-RNA interactions, requiring only nanomolar concentrations of labeled components. Fluorescence anisotropy provides information about the assembly of multi-subunit proteins, while molecular beacons provide a sensitive and quantitative reporter for base pairing between complementary RNAs. Here we present a detailed protocol for labeling Hfq protein with cyanine 3-maleimide and dansyl chloride to...

  12. Positioning system in wireless sensor networks using NS-2

    CSIR Research Space (South Africa)

    Abu-Mahfouz, Adnan M

    2012-10-01

    Full Text Available and techniques require that the positions of the sensor nodes be determined. Localisation algorithms (e.g.[1-5]) follow several approaches to estimate positions of sensor nodes. One approach is to use special nodes, called beacons, which know their own... and analysing its performance have made simulation essential for the study of WSNs. Simulation is widely used in system modelling for applications ranging from engineering research, business analysis, manufacturing planning and biological science...

  13. Adult lives: a life course perspective

    National Research Council Canada - National Science Library

    Katz, Jeanne; Peace, Sheila M; Spurr, Sue

    2012-01-01

    ... learn more about the wide range of modules and qualifications offered at all levels by The Open University.Adult lives A life course perspective Edited by Jeanne Katz, Sheila Peace and Sue SpurrFirst published in 2012 by The Policy Press, University of Bristol, Fourth Floor, Beacon House, Queen's Road, Bristol BS8 1QU, UK www.policypress.co.uk in asso...

  14. 道路粉じんによる大気汚染実態把握のための基礎的検討(第1報) : 多元素分析による指標元素の検索

    OpenAIRE

    福崎, 紀夫; 谷中, 隆明; 漆山, 佳雄; 霜鳥, 達雄; 市川, 義夫; Fukuzaki, Norio; Yanaka, Takaaki; Urushiyama, Yoshio; Shimotori, Tatuo; Ichikawa, Yoshio

    1983-01-01

    Non-destructive neutron activation analysis was applied to a research of indicator elements in asphalt, diesel and gasoline engine soot, tire tread, tire stud and road beacon paint that were considered to be structure materials of roadway dust. From the analysis of 38 elements, suitable indicator elements were found, i.e. bromine in gasloline engine soot, tungsten in tire stud tip and chromium in yellow paint but no suitable indicator elements was found in asphalt, tire tread and diesel engin...

  15. Grief Reporting: A Print Media Content Analysis of the Gander, Newfoundland Air Disaster

    Science.gov (United States)

    1988-03-11

    Press, 1986). McQuail , Denis. Mass Communication Theory . (Beverly Hills, California: Sage Publications, 1984). Mencher, Melvin. News Reporting and...Adversaries. (Boston: Beacon Press, 1970). Rivers, William L. and Wilbur Schramm. Responsibility in Mass Communication . Rev. ed. (New -York, N.Y...NUMBERS Grief Reporting: A Print Media Content Analysis of the Gander, Newfoundland Air Disaster 6. AUTHOR( S ) Captain Barbara Ann Goodno 7. PERFORMING

  16. JPRS Report, Science and Technology: Europe Twenty-Fourth ISATA International Symposium on Automotive Technology and Automation.

    Science.gov (United States)

    1991-09-05

    Traffic Research Centre VSC, The Netherlands 910061 " Adaptability in Driver Support" M J Kuiken, PGM Miltenburg, 733 Traffic Research Centre, The...Local C Control C Centre R Road S Side S Station /tag*/ Traffic Lights Variable Message Signs Beacons P’R a On-Board IHt Celular Rade...are not suitable for automatic debiting, nevertheless the same technological approach can be adapted to the stated requirements . 1*3 The DACAR

  17. Role of cyclones and other factors in the decline of the ports of northern Orissa

    Digital Repository Service at National Institute of Oceanography (India)

    Tripati, S.; Unnikrishnan, A.S.

    because marked buoys and beacons were provided in the mid channel of the rivers for navigation 4 . Churamani and Laichanpur The Churamani and Laichanpur ports were on the banks of the rivers Gammai and Kamsabamsa. Of these two ports, the greater... to Bengal, and the Europeans established their trade centres in different parts of Bengal. This indicates the withdrawal of royal patronage from trade and com- merce. The shifting of headquarters from Balasore to Hugli also affected the commercial...

  18. Enabling Wireless Power Transfer in Cellular Networks: Architecture, Modeling and Deployment

    OpenAIRE

    Huang, Kaibin; Lau, Vincent K. N.

    2012-01-01

    Microwave power transfer (MPT) delivers energy wirelessly from stations called power beacons (PBs) to mobile devices by microwave radiation. This provides mobiles practically infinite battery lives and eliminates the need of power cords and chargers. To enable MPT for mobile charging, this paper proposes a new network architecture that overlays an uplink cellular network with randomly deployed PBs for powering mobiles, called a hybrid network. The deployment of the hybrid network under an out...

  19. Trajectory Planning for Nonholonomic Mobile Robot Using Extended Kalman Filter

    OpenAIRE

    de Melo, Leonimer Flavio; Junior, Jose Fernando Mangili

    2010-01-01

    In the mobile robotic systems, a precise estimate of the robot pose with the intention of the optimization in the path planning is essential for the correct performance, on the part of the robots, for tasks that are destined to it. This paper describes the use of RF digital signal interacting with beacons for computational triangulation in the way to provide a pose estimative at bidimensional indoor environment, where GPS system is out of range. This methodology takes advantage...

  20. Range Environmental Assessment for Test Area C-52 Complex, Eglin Air Force Base, Florida

    Science.gov (United States)

    2014-10-31

    tourism in the region. Promotes and develops general business, trade, and tourism components of the state economy. Chapter 334 Transportation... Shopping Center across from PoFolks) Comfort Keepers is looking for reliable caregivers for our sen- ior clients. CNA a plus. Apply at ck340.ersp.biz...John Sims Pkwy., Parkway East Shopping Center. Hours: 8 a.m.- 5 p.m. M-F. After hours, use mail slot in our door. MAIL: Beacon Newspapers, 1181 E

  1. Journal of the American Society for Information Science (JASIS). Volume 49, Number 13

    Science.gov (United States)

    1998-01-01

    plans. This text constitutes what may be called the infra- municative action ( Habermas , 1984, 1987). Communicative structure of the organization...influence on the structure of newspaper organizations. Academy of Habermas , J. (1984). The theory of communicative action, Vol. 1. Boston: Management...Journal, 27(2), 247-270. Beacon Press. Castels, M. (1986). High technology, world development, and structural Habermas , J. (1987). The theory of

  2. An Autonomous Distributed Fault-Tolerant Local Positioning System

    Science.gov (United States)

    Malekpour, Mahyar R.

    2017-01-01

    We describe a fault-tolerant, GPS-independent (Global Positioning System) distributed autonomous positioning system for static/mobile objects and present solutions for providing highly-accurate geo-location data for the static/mobile objects in dynamic environments. The reliability and accuracy of a positioning system fundamentally depends on two factors; its timeliness in broadcasting signals and the knowledge of its geometry, i.e., locations and distances of the beacons. Existing distributed positioning systems either synchronize to a common external source like GPS or establish their own time synchrony using a scheme similar to a master-slave by designating a particular beacon as the master and other beacons synchronize to it, resulting in a single point of failure. Another drawback of existing positioning systems is their lack of addressing various fault manifestations, in particular, communication link failures, which, as in wireless networks, are increasingly dominating the process failures and are typically transient and mobile, in the sense that they typically affect different messages to/from different processes over time.

  3. Status of ARGOS - The Laser Guide Star System for the LBT

    Science.gov (United States)

    Raab, Walfried; Rabien, Sebastian; Gaessler, Wolfgang; Esposito, Simone; Antichi, Jacopo; Lloyd-Hart, Michael; Barl, Lothar; Beckmann, Udo; Bonaglia, Marco; Borelli, Jose; Brynnel, Joar; Buschkamp, Peter; Busoni, Lorenzo; Carbonaro, Luca; Christou, Julian; Connot, Claus; Davies, Richard; Deysenroth, Matthias; Durney, Olivier; Green, Richard; Gemperlein, Hans; Gasho, Victor; Haug, Marcus; Hubbard, Pete; Ihle, Sebastian; Kulas, Martin; Loose, Christina; Lehmitz, Michael; Noenickx, Jamison; Nussbaum, Edmund; Orban De Xivry, Gilles; Quirrenbach, Andreas; Peter, Diethard; Rahmer, Gustavo; Rademacher, Matt; Storm, Jesper; Schwab, Christian; Vaitheeswaran, Vidhya; Ziegleder, Julian

    2013-12-01

    ARGOS is an innovative multiple laser guide star adaptive optics system for the Large Binocular Telescope (LBT), designed to perform effective GLAO correction over a very wide field of view. The system is using high powered pulsed green (532 nm) lasers to generate a set of three guide stars above each of the LBT mirrors. The laser beams are launched through a 40 cm telescope and focused at an altitude of 12 km, creating laser beacons by means of Rayleigh scattering. The returning scattered light, primarily sensitive to the turbulences close to the ground, is detected by a gated wavefront sensor system. The derived ground layer correction signals are directly driving the adaptive secondary mirror of the LBT. ARGOS is especially designed for operation with the multiple object spectrograph Luci, which will benefit from both, the improved spatial resolution, as well as the strongly enhanced flux. In addition to the GLAO Rayleigh beacon system, ARGOS was also designed for a possible future upgrade with a hybrid sodium laser - Rayleigh beacon combination, enabling diffraction limited operation. The ARGOS laser system has undergone extensive tests during Summer 2012 and is scheduled for installation at the LBT in Spring 2013. The remaining sub-systems will be installed during the course of 2013. We report on the overall status of the ARGOS system and the results of the sub-system characterizations carried out so far.

  4. An Empirical Study of the Transmission Power Setting for Bluetooth-Based Indoor Localization Mechanisms.

    Science.gov (United States)

    Castillo-Cara, Manuel; Lovón-Melgarejo, Jesús; Bravo-Rocca, Gusseppe; Orozco-Barbosa, Luis; García-Varea, Ismael

    2017-06-07

    Nowadays, there is a great interest in developing accurate wireless indoor localization mechanisms enabling the implementation of many consumer-oriented services. Among the many proposals, wireless indoor localization mechanisms based on the Received Signal Strength Indication (RSSI) are being widely explored. Most studies have focused on the evaluation of the capabilities of different mobile device brands and wireless network technologies. Furthermore, different parameters and algorithms have been proposed as a means of improving the accuracy of wireless-based localization mechanisms. In this paper, we focus on the tuning of the RSSI fingerprint to be used in the implementation of a Bluetooth Low Energy 4.0 (BLE4.0) Bluetooth localization mechanism. Following a holistic approach, we start by assessing the capabilities of two Bluetooth sensor/receiver devices. We then evaluate the relevance of the RSSI fingerprint reported by each BLE4.0 beacon operating at various transmission power levels using feature selection techniques. Based on our findings, we use two classification algorithms in order to improve the setting of the transmission power levels of each of the BLE4.0 beacons. Our main findings show that our proposal can greatly improve the localization accuracy by setting a custom transmission power level for each BLE4.0 beacon.

  5. An Empirical Study of the Transmission Power Setting for Bluetooth-Based Indoor Localization Mechanisms

    Directory of Open Access Journals (Sweden)

    Manuel Castillo-Cara

    2017-06-01

    Full Text Available Nowadays, there is a great interest in developing accurate wireless indoor localization mechanisms enabling the implementation of many consumer-oriented services. Among the many proposals, wireless indoor localization mechanisms based on the Received Signal Strength Indication (RSSI are being widely explored. Most studies have focused on the evaluation of the capabilities of different mobile device brands and wireless network technologies. Furthermore, different parameters and algorithms have been proposed as a means of improving the accuracy of wireless-based localization mechanisms. In this paper, we focus on the tuning of the RSSI fingerprint to be used in the implementation of a Bluetooth Low Energy 4.0 (BLE4.0 Bluetooth localization mechanism. Following a holistic approach, we start by assessing the capabilities of two Bluetooth sensor/receiver devices. We then evaluate the relevance of the RSSI fingerprint reported by each BLE4.0 beacon operating at various transmission power levels using feature selection techniques. Based on our findings, we use two classification algorithms in order to improve the setting of the transmission power levels of each of the BLE4.0 beacons. Our main findings show that our proposal can greatly improve the localization accuracy by setting a custom transmission power level for each BLE4.0 beacon.

  6. A Localization Method for Underwater Wireless Sensor Networks Based on Mobility Prediction and Particle Swarm Optimization Algorithms

    Directory of Open Access Journals (Sweden)

    Ying Zhang

    2016-02-01

    Full Text Available Due to their special environment, Underwater Wireless Sensor Networks (UWSNs are usually deployed over a large sea area and the nodes are usually floating. This results in a lower beacon node distribution density, a longer time for localization, and more energy consumption. Currently most of the localization algorithms in this field do not pay enough consideration on the mobility of the nodes. In this paper, by analyzing the mobility patterns of water near the seashore, a localization method for UWSNs based on a Mobility Prediction and a Particle Swarm Optimization algorithm (MP-PSO is proposed. In this method, the range-based PSO algorithm is used to locate the beacon nodes, and their velocities can be calculated. The velocity of an unknown node is calculated by using the spatial correlation of underwater object’s mobility, and then their locations can be predicted. The range-based PSO algorithm may cause considerable energy consumption and its computation complexity is a little bit high, nevertheless the number of beacon nodes is relatively smaller, so the calculation for the large number of unknown nodes is succinct, and this method can obviously decrease the energy consumption and time cost of localizing these mobile nodes. The simulation results indicate that this method has higher localization accuracy and better localization coverage rate compared with some other widely used localization methods in this field.

  7. A Localization Method for Underwater Wireless Sensor Networks Based on Mobility Prediction and Particle Swarm Optimization Algorithms.

    Science.gov (United States)

    Zhang, Ying; Liang, Jixing; Jiang, Shengming; Chen, Wei

    2016-02-06

    Due to their special environment, Underwater Wireless Sensor Networks (UWSNs) are usually deployed over a large sea area and the nodes are usually floating. This results in a lower beacon node distribution density, a longer time for localization, and more energy consumption. Currently most of the localization algorithms in this field do not pay enough consideration on the mobility of the nodes. In this paper, by analyzing the mobility patterns of water near the seashore, a localization method for UWSNs based on a Mobility Prediction and a Particle Swarm Optimization algorithm (MP-PSO) is proposed. In this method, the range-based PSO algorithm is used to locate the beacon nodes, and their velocities can be calculated. The velocity of an unknown node is calculated by using the spatial correlation of underwater object's mobility, and then their locations can be predicted. The range-based PSO algorithm may cause considerable energy consumption and its computation complexity is a little bit high, nevertheless the number of beacon nodes is relatively smaller, so the calculation for the large number of unknown nodes is succinct, and this method can obviously decrease the energy consumption and time cost of localizing these mobile nodes. The simulation results indicate that this method has higher localization accuracy and better localization coverage rate compared with some other widely used localization methods in this field.

  8. Relay mirror experiment

    Science.gov (United States)

    Begley, David L.

    1996-04-01

    Originating out of a SDIO-funded, Phase 1 study effort, two ground systems and an orbiting EO payload/spacecraft were the primary equipment for the RME. The RME was originally conceived to be a shuttle deployed experiment. Shortly after program start, the Challenger disaster occurred, with the promise of extensive delays. A completely new space segment was to be designed incorporating a free-flying spacecraft. During the midphase of the program, a variety of launch vehicles were envisioned to replace the shuttle, requiring the BASD team to design accommodations for Delta, Atlas, and Titan, with a Delta launch being the final solution. The ground systems tracked the spacecraft and illuminated it with green and blue beacon lasers. The Payload Experiment Package (PEP) housed the bisection tracker, a key innovation central to the experiment. The bisection tracker acquired both beacons and controlled steerable mirrors to accomplish fine tracking of the two cooperative beacons. In the process, the relay mirror was precisely positioned to enable a successful relay of a third infrared laser between the two ground sites via the orbiting spacecraft. Many of the key technologies employed in the PEP were originally developed for Ball laser communications research and development programs and other laser pointing efforts. The WAVE sensor package, built by ATA and integrated by Ball, measured the vibrations of the optical base structure on which it was mounted. These spacecraft vibration data are critical to the accurate pointing of space laser communication terminals.

  9. Filtering Method for Location Estimation of an Underwater Robot

    Directory of Open Access Journals (Sweden)

    Nak Yong Ko

    2014-05-01

    Full Text Available This paper describes an application of extended Kalman filter(EKF for localization of an underwater robot. For the application, linearized model of robot motion and sensor measurement are derived. Like usual EKF, the method is recursion of two main steps: the time update(or prediction and measurement update. The measurement update uses exteroceptive sensors such as four acoustic beacons and a pressure sensor. The four beacons provide four range data from these beacons to the robot and pressure sensor does the depth data of the robot. One of the major contributions of the paper is suggestion of two measurement update approaches. The first approach corrects the predicted states using the measurement data individually. The second one corrects the predicted state using the measurement data collectively. The simulation analysis shows that EKF outperforms least squares or odometry based dead-reckoning in the precision and robustness of the estimation. Also, EKF with collective measurement update brings out better accuracy than the EKF with individual measurement update.

  10. The trillion planet survey: an optical search for directed intelligence in M31

    Science.gov (United States)

    Stewart, Andrew; Lubin, Philip

    2017-09-01

    In realm of optical SETI, searches for pulsed laser signals have historically been preferred over those for continuous wave beacons. There are many valid reasons for this, namely the near elimination of false positives and simple experimental components. However, due to significant improvements in laser technologies and light-detection systems since the mid-20th century, as well as new data from the recent Kepler mission, continuous wave searches should no longer be ignored. In this paper we propose a search for continuous wave laser beacons from an intelligent civilization in the Andromeda galaxy. Using only a 0.8 meter telescope, a standard photometric system, and an image processing pipeline, we expect to be able to detect any CW laser signal directed at us from an extraterrestrial civilization in M31, as long as the civilization is operating at a wavelength we can "see" and has left the beacon on long enough for us to detect it here on Earth. The search target is M31 due to its high stellar density relative to our own Milky Way galaxy. Andromeda is home to at least one trillion stars, and thus at least one trillion planets. As a result, in surveying M31, we are surveying one trillion planets, and consequently one trillion possible locations of intelligent life. This is an unprecedented number of targets relative to other past SETI searches. We call this the TPS or Trillion Planet Survey.

  11. 20 MW Flywheel frequency regulation plant

    Energy Technology Data Exchange (ETDEWEB)

    Arseneaux, James [Beacon Power LLC, Wilmington, MA (United States)

    2015-02-05

    Hazle designed, built, commissioned, and operates a utility-scale 20 MW flywheel energy storage plant in Hazle Township, Pennsylvania (the Hazle Facility) using flywheel technology developed by its affiliate, Beacon Power, LLC (Beacon Power). The Hazle Facility provides frequency regulation services to the regional transmission organization, PJM Interconnection, LLC (PJM), through its participation in PJM’s Regulation Market (a market-based system for the purchase and sale of the Regulation ancillary service). The zero emission Hazle Facility is designed for a 20 year-life over which it is capable of performing at least 100,000 full depth of discharge cycles. To achieve its 20 MW capacity, the Hazle Facility is comprised of two hundred of Beacon Power’s 100 kilowatt (kW)/25 kilowatt/hour (kWh) flywheels connected in parallel. The Hazle Facility can fully respond to a signal from PJM in less than 2 seconds. The Hazle facility was constructed in an economic development zone designated by the Commonwealth of Pennsylvania and its construction relied on local contractors and labor for completion.

  12. Benefits from flywheel energy storage for area regulation in California - demonstration results : a study for the DOE Energy Storage Systems program.

    Energy Technology Data Exchange (ETDEWEB)

    Eyer, James M. (Distributed Utility Associates, Livermore, CA)

    2009-10-01

    This report documents a high-level analysis of the benefit and cost for flywheel energy storage used to provide area regulation for the electricity supply and transmission system in California. Area regulation is an 'ancillary service' needed for a reliable and stable regional electricity grid. The analysis was based on results from a demonstration, in California, of flywheel energy storage developed by Beacon Power Corporation (the system's manufacturer). Demonstrated was flywheel storage systems ability to provide 'rapid-response' regulation. Flywheel storage output can be varied much more rapidly than the output from conventional regulation sources, making flywheels more attractive than conventional regulation resources. The performance of the flywheel storage system demonstrated was generally consistent with requirements for a possible new class of regulation resources - 'rapid-response' energy-storage-based regulation - in California. In short, it was demonstrated that Beacon Power Corporation's flywheel system follows a rapidly changing control signal (the ACE, which changes every four seconds). Based on the results and on expected plant cost and performance, the Beacon Power flywheel storage system has a good chance of being a financially viable regulation resource. Results indicate a benefit/cost ratio of 1.5 to 1.8 using what may be somewhat conservative assumptions. A benefit/cost ratio of one indicates that, based on the financial assumptions used, the investment's financial returns just meet the investors target.

  13. On Event Detection and Localization in Acyclic Flow Networks

    KAUST Repository

    Suresh, Mahima Agumbe

    2013-05-01

    Acyclic flow networks, present in many infrastructures of national importance (e.g., oil and gas and water distribution systems), have been attracting immense research interest. Existing solutions for detecting and locating attacks against these infrastructures have been proven costly and imprecise, particularly when dealing with large-scale distribution systems. In this article, to the best of our knowledge, for the first time, we investigate how mobile sensor networks can be used for optimal event detection and localization in acyclic flow networks. We propose the idea of using sensors that move along the edges of the network and detect events (i.e., attacks). To localize the events, sensors detect proximity to beacons, which are devices with known placement in the network. We formulate the problem of minimizing the cost of monitoring infrastructure (i.e., minimizing the number of sensors and beacons deployed) in a predetermined zone of interest, while ensuring a degree of coverage by sensors and a required accuracy in locating events using beacons. We propose algorithms for solving the aforementioned problem and demonstrate their effectiveness with results obtained from a realistic flow network simulator.

  14. Radioactivity survey in Antarctica, 1978-1979

    Energy Technology Data Exchange (ETDEWEB)

    Zeller, E. (Univ. of Kansas, Lawrence); Dreschhoff, G.; Thoste, V.; Kropp, W.R.

    1979-10-01

    The 1978-79 season proved to be particularly successful for the Antarctic International Radiometric Survey (AIRS Project), which was based at the Darwin Glacier Camp. We detected a significant radiation anomaly near Touchdown Glacier. The source of radioactivity was found to be thorium-bearing minerals in a placer-type deposit in the Brown Hills conglomerate member of the Windy Gully sandstone. Extensive outcrops of the basement rocks that underlie the Beacon Supergroup were found at the base of Tentacle Ridge. These rocks are mineralized around a number of narrow veins and they are intensely weathered below the contact with the overlying Beacon Supergroup. As the survey was extended south of the head of Byrd Glacier into the area of the Sefton and Zeller glaciers, a complex series of low-grade metamorphic rocks was encountered overlain by Beacon Supergroup sediments. Near the conclusion of airborne survey operations, a flight was made to Butcher Ridge in the northwestern corner of the survey area. A layered intrusive body was discovered that outcrops for nearly 10 kilometers along the east face of the ridge.

  15. Method to improve the survival of night-swarming mayflies near bridges in areas of distracting light pollution.

    Science.gov (United States)

    Egri, Ádám; Száz, Dénes; Farkas, Alexandra; Pereszlényi, Ádám; Horváth, Gábor; Kriska, György

    2017-11-01

    Numerous negative ecological effects of urban lighting have been identified during the last decades. In spite of the development of lighting technologies, the detrimental effect of this form of light pollution has not declined. Several insect species are affected including the night-swarming mayfly Ephoron virgo : when encountering bridges during their mass swarming, these mayflies often fall victim to artificial lighting. We show a simple method for the conservation of these mayflies exploiting their positive phototaxis. With downstream-facing light-emitting diode beacon lights above two tributaries of the river Danube, we managed to guide egg-laying females to the water and prevent them from perishing outside the river near urban lights. By means of measuring the mayfly outflow from the river as a function of time and the on/off state of the beacons, we showed that the number of mayflies exiting the river's area was practically zero when our beacons were operating. Tributaries could be the sources of mayfly recolonization in case of water quality degradation of large rivers. The protection of mayfly populations in small rivers and safeguarding their aggregation and oviposition sites is therefore important.

  16. SIMULATION OF WIRELESS SENSOR NETWORK WITH HYBRID TOPOLOGY

    Directory of Open Access Journals (Sweden)

    J. Jaslin Deva Gifty

    2016-03-01

    Full Text Available The design of low rate Wireless Personal Area Network (WPAN by IEEE 802.15.4 standard has been developed to support lower data rates and low power consuming application. Zigbee Wireless Sensor Network (WSN works on the network and application layer in IEEE 802.15.4. Zigbee network can be configured in star, tree or mesh topology. The performance varies from topology to topology. The performance parameters such as network lifetime, energy consumption, throughput, delay in data delivery and sensor field coverage area varies depending on the network topology. In this paper, designing of hybrid topology by using two possible combinations such as star-tree and star-mesh is simulated to verify the communication reliability. This approach is to combine all the benefits of two network model. The parameters such as jitter, delay and throughput are measured for these scenarios. Further, MAC parameters impact such as beacon order (BO and super frame order (SO for low power consumption and high channel utilization, has been analysed for star, tree and mesh topology in beacon disable mode and beacon enable mode by varying CBR traffic loads.

  17. Optimizing the MAC Protocol in Localization Systems Based on IEEE 802.15.4 Networks

    Directory of Open Access Journals (Sweden)

    Juan J. Pérez-Solano

    2017-07-01

    Full Text Available Radio frequency signals are commonly used in the development of indoor localization systems. The infrastructure of these systems includes some beacons placed at known positions that exchange radio packets with users to be located. When the system is implemented using wireless sensor networks, the wireless transceivers integrated in the network motes are usually based on the IEEE 802.15.4 standard. But, the CSMA-CA, which is the basis for the medium access protocols in this category of communication systems, is not suitable when several users want to exchange bursts of radio packets with the same beacon to acquire the radio signal strength indicator (RSSI values needed in the location process. Therefore, new protocols are necessary to avoid the packet collisions that appear when multiple users try to communicate with the same beacons. On the other hand, the RSSI sampling process should be carried out very quickly because some systems cannot tolerate a large delay in the location process. This is even more important when the RSSI sampling process includes measures with different signal power levels or frequency channels. The principal objective of this work is to speed up the RSSI sampling process in indoor localization systems. To achieve this objective, the main contribution is the proposal of a new MAC protocol that eliminates the medium access contention periods and decreases the number of packet collisions to accelerate the RSSI collection process. Moreover, the protocol increases the overall network throughput taking advantage of the frequency channel diversity. The presented results show the suitability of this protocol for reducing the RSSI gathering delay and increasing the network throughput in simulated and real environments.

  18. Integration and Evaluation of a Position Sensor with Continuous Read-Out for use with the Environmental Measurement-While-Drilling Gamma Ray Spectrometer System

    International Nuclear Information System (INIS)

    Lockwood, G.J.; Normann, R.A.; Selph, M.M.; Williams, C.V.

    1999-01-01

    The Environmental Measurement-While-Drilling-Gamma Ray Spectrometer (EMWD-GRS) system represents an innovative blend of new and existing technology that provides real-time environmental and drill bit data during drilling operations. The EMWD-GRS technology was demonstrated at Savannah River Site (SRS) F-Area Retention Basin. The EMWD-GRS technology demonstration consisted of continuously monitoring for gamma-radiation-producing contamination while drilling two horizontal boreholes below the backfilled waste retention basin. These boreholes passed near previously sampled locations where concentrations of contaminant levels of cesium had been measured. Contaminant levels continuously recorded by the EMWD-GRS system during drilling were compared to contaminant levels previously determined through quantitative laboratory analysis of soil samples. The demonstration of the EMWD-GRS was a complete success. The results show general agreement between the soil sampling and EMWD-GRS techniques for CS-137. It was recognized that the EMWD-GRS tool would better satisfy our customers' needs if the instrument location could be continuously monitored. During the demonstration at SRS, an electromagnetic beacon with a walkover monitor (Subsitereg s ign) was used to measure bit location at depth. To use a beacon locator drilling must be stopped, thus it is normally only used when a new section of pipe was added. The location of contamination could only be estimated based on the position of the EMED-GRS package and the distance between locator beacon readings. A continuous location system that would allow us to know the location of each spectrum as it is obtained is needed

  19. Ultrasound user-identification for wireless sensor networks.

    Science.gov (United States)

    Shahid, Bilal; Kannan, Anushiya A; Lovell, Nigel H; Redmond, Stephen J

    2010-01-01

    Indoor localization and asset tracking is an area of research which is finding increasing application in monitoring the wellbeing of elderly subjects in hospital or residential care environments. Radio frequency (RF) based localization methods tend to show poor performance in indoor environments, due to multipath scatter, and simple room-level misassociations, resulting from the relative transparency of some walls to RF waves. Ultrasound (US) localization has shown excellent room-level accuracy, and as US will not easily penetrate walls, room-level misassociations do not often occur. For normal asset tracking systems, the association between an asset and a room is not of critical importance; for clinical applications, which make inferences based on a patient and carer occupying the same room, this association is paramount. This paper outlines a proof of concept for an US-based indoor localization system with sub-room-level accuracy. The proposed design uses a US-based technique, with transmitter beacons placed throughout the environment. The subject wears the receiver module and is free to move throughout the environment. Transmission collisions are alleviated by assigning a unique prime transmission interval to each beacon; meaning the beacons do not require synchronization or coordination and operate independently of one another. Preliminary results show accurate detection at a range of over 5 m at a 0° angle and at approximately 3.5 m at an angle of 30°. Testing of two collocated transmitters, transmitting in the same plane and separated by 2.5 m results in accurate reception of both signals, even within the region of overlap.

  20. Optimizing the MAC Protocol in Localization Systems Based on IEEE 802.15.4 Networks.

    Science.gov (United States)

    Pérez-Solano, Juan J; Claver, Jose M; Ezpeleta, Santiago

    2017-07-06

    Radio frequency signals are commonly used in the development of indoor localization systems. The infrastructure of these systems includes some beacons placed at known positions that exchange radio packets with users to be located. When the system is implemented using wireless sensor networks, the wireless transceivers integrated in the network motes are usually based on the IEEE 802.15.4 standard. But, the CSMA-CA, which is the basis for the medium access protocols in this category of communication systems, is not suitable when several users want to exchange bursts of radio packets with the same beacon to acquire the radio signal strength indicator (RSSI) values needed in the location process. Therefore, new protocols are necessary to avoid the packet collisions that appear when multiple users try to communicate with the same beacons. On the other hand, the RSSI sampling process should be carried out very quickly because some systems cannot tolerate a large delay in the location process. This is even more important when the RSSI sampling process includes measures with different signal power levels or frequency channels. The principal objective of this work is to speed up the RSSI sampling process in indoor localization systems. To achieve this objective, the main contribution is the proposal of a new MAC protocol that eliminates the medium access contention periods and decreases the number of packet collisions to accelerate the RSSI collection process. Moreover, the protocol increases the overall network throughput taking advantage of the frequency channel diversity. The presented results show the suitability of this protocol for reducing the RSSI gathering delay and increasing the network throughput in simulated and real environments.

  1. A Robust High-Accuracy Ultrasound Indoor Positioning System Based on a Wireless Sensor Network

    Directory of Open Access Journals (Sweden)

    Jun Qi

    2017-11-01

    Full Text Available This paper describes the development and implementation of a robust high-accuracy ultrasonic indoor positioning system (UIPS. The UIPS consists of several wireless ultrasonic beacons in the indoor environment. Each of them has a fixed and known position coordinate and can collect all the transmissions from the target node or emit ultrasonic signals. Every wireless sensor network (WSN node has two communication modules: one is WiFi, that transmits the data to the server, and the other is the radio frequency (RF module, which is only used for time synchronization between different nodes, with accuracy up to 1 μs. The distance between the beacon and the target node is calculated by measuring the time-of-flight (TOF for the ultrasonic signal, and then the position of the target is computed by some distances and the coordinate of the beacons. TOF estimation is the most important technique in the UIPS. A new time domain method to extract the envelope of the ultrasonic signals is presented in order to estimate the TOF. This method, with the envelope detection filter, estimates the value with the sampled values on both sides based on the least squares method (LSM. The simulation results show that the method can achieve envelope detection with a good filtering effect by means of the LSM. The highest precision and variance can reach 0.61 mm and 0.23 mm, respectively, in pseudo-range measurements with UIPS. A maximum location error of 10.2 mm is achieved in the positioning experiments for a moving robot, when UIPS works on the line-of-sight (LOS signal.

  2. Laser Calibration Experiment for Small Objects in Space

    Science.gov (United States)

    Campbell, Jonathan; Ayers, K.; Carreras, R.; Carruth, R.; Freestone, T.; Sharp, J.; Rawleigh, A.; Brewer, J.; Schrock, K.; Bell, L.; hide

    2001-01-01

    The Air Force Research Laboratory/Directed Energy Directorate (AFRL/DE) and NASA/Marshall Space Flight Center (MSFC) are looking at a series of joint laser space calibration experiments using the 12J 15Hz CO2 High Performance CO2 Ladar Surveillance Sensor (FU-CLASS) system on the 3.67 meter aperture Advanced Electro-Optics System (AEOS). The objectives of these experiments are to provide accurate range and signature measurements of calibration spheres, demonstrate high resolution tracking capability of small objects, and support NASA in technology development and tracking projects. Ancillary benefits include calibrating radar and optical sites, completing satellite conjunction analyses, supporting orbital perturbations analyses, and comparing radar and optical signatures. In the first experiment, a Global Positioning System (GPS)/laser beacon instrumented microsatellite about 25 cm in diameter will be deployed from a Space Shuttle Hitchhiker canister or other suitable launch means. Orbiting in low earth orbit, the microsatellite will pass over AEOS on the average of two times per 24-hour period. An onboard orbit propagator will activate the GPS unit and a visible laser beacon at the appropriate times. The HI-CLASS/AEOS system will detect the microsatellite as it rises above the horizon, using GPS-generated acquisition vectors. The visible laser beacon will be used to fine-tune the tracking parameters for continuous ladar data measurements throughout the pass. This operational approach should maximize visibility to the ground-based laser while allowing battery life to be conserved, thus extending the lifetime of the satellite. GPS data will be transmitted to the ground providing independent location information for the microsatellite down to sub-meter accuracies.

  3. Impact of Scattering Model on Disdrometer Derived Attenuation Scaling

    Science.gov (United States)

    Zemba, Michael; Luini, Lorenzo; Nessel, James; Riva, Carlo (Compiler)

    2016-01-01

    NASA Glenn Research Center (GRC), the Air Force Research Laboratory (AFRL), and the Politecnico di Milano (POLIMI) are currently entering the third year of a joint propagation study in Milan, Italy utilizing the 20 and 40 GHz beacons of the Alphasat TDP5 Aldo Paraboni scientific payload. The Ka- and Q-band beacon receivers were installed at the POLIMI campus in June of 2014 and provide direct measurements of signal attenuation at each frequency. Collocated weather instrumentation provides concurrent measurement of atmospheric conditions at the receiver; included among these weather instruments is a Thies Clima Laser Precipitation Monitor (optical disdrometer) which records droplet size distributions (DSD) and droplet velocity distributions (DVD) during precipitation events. This information can be used to derive the specific attenuation at frequencies of interest and thereby scale measured attenuation data from one frequency to another. Given the ability to both predict the 40 GHz attenuation from the disdrometer and the 20 GHz timeseries as well as to directly measure the 40 GHz attenuation with the beacon receiver, the Milan terminal is uniquely able to assess these scaling techniques and refine the methods used to infer attenuation from disdrometer data.In order to derive specific attenuation from the DSD, the forward scattering coefficient must be computed. In previous work, this has been done using the Mie scattering model, however, this assumes a spherical droplet shape. The primary goal of this analysis is to assess the impact of the scattering model and droplet shape on disdrometer derived attenuation predictions by comparing the use of the Mie scattering model to the use of the T-matrix method, which does not assume a spherical droplet. In particular, this paper will investigate the impact of these two scattering approaches on the error of the resulting predictions as well as on the relationship between prediction error and rain rate.

  4. The Evolution and Fate of Saturn's Stratospheric Vortex: Infrared Spectroscopy from Cassini

    Science.gov (United States)

    Fletcher, Leigh N.; Hesman, B. E.; Arhterberg, R. K.; Bjoraker, G.; Irwin, P. G. J.; Hurley, J.; Sinclair, J.; Gorius, N.; Orton, G. S.; Read, P. L.; hide

    2012-01-01

    The planet-encircling springtime storm in Saturn's troposphere (December 2010-July 2011) produced dramatic perturbations to stratospheric temperatures, winds and composition at mbar pressures that persisted long after the tropospheric disturbance had abated. Observations from the Cassini Composite Infrared Spectrometer (CIRS), supported by ground-based imaging from the VISIR instrument on the Very Large Telescope,is used to track the evolution of a large, hot stratospheric anticyclone between January 2011 and the present day. The evolutionary sequence can be divided into three phases: (I) the formation and intensification of two distinct warm airmasses near 0.5 mbar between 25 and 35N (one residing directly above the convective storm head) between January-April 2011, moving westward with different zonal velocities; (II) the merging of the warm airmasses to form the large single 'stratospheric beacon' near 40N between April and June 2011, dissociated from the storm head and at a higher pressure (2 mbar) than the original beacons; and (III) the mature phase characterized by slow cooling and longitudinal shrinkage of the anticyclone since July 2011, moving west with a near-constant velocity of 2.70+/-0.04 deg/day (-24.5+/-0.4 m/s at 40N). Peak temperatures of 220 K at 2 mbar were measured on May 5th 2011 immediately after the merger, some 80 K warmer than the quiescent surroundings. Thermal winds hear calculations in August 2011 suggest clockwise peripheral velocities of 200400 mls at 2 mbar, defining a peripheral collar with a width of 65 degrees longitude (50,000 km in diameter) and 25 degrees latitude. Stratospheric acetylene (C2H2) was uniformly enhanced by a factor of three within the vortex, whereas ethane (C2H6) remained unaffected. We will discuss the thermal and chemical characteristics of Saturn's beacon in its mature phase, and implications for stratospheric vortices on other giant planets.

  5. The concept of beauty in the Serbian language - iconicity of lexemes ‘lepo’ and ‘krasno’

    Directory of Open Access Journals (Sweden)

    Bašić Ivana S.

    2014-01-01

    Full Text Available ‘Beauty’ - the crucial aesthetic category is conceived in the Serbian language by two lexemes: ‘lepo’ and ‘krasno’. Iconicity of lexeme ‘lepo’ (Old Church Slavonic - lЪp'' “something that is bonded with something; mud, mortar or most often mud mixed up with chuff to ‘glue with’ or ‘spread over with’ is linked with the imagination of soil, while the concept of beautiful is conceived by lexeme ‘krasno’ (I.E.*(sqer-, “beacon fire“ and is defined by heavenly and divine splendour. The difference in iconicity of the two lexemes, in essence, is the difference between terrestrial and heavenly beauties. The terrestrial beauty is defined by the creative innate impulse in humans to guide them towards amorphous but “binding“ matter that can be sculptured into ‘beautiful’ things with a function that is not necessarily utilitarian - but perhaps it had a sacramental character. On contrary, the heavenly beauty exemplifies manifestation of heavenly splendour - beacon of divinity, beacon of creativeness and effervescence which presents the terrestrial world in its glorious beauty. The picture of heavenly ‘fire’, quite independently form the benefit it may bring us, especially from the benefit that may be gained from the terrestrial fire, as well as the pure aesthetical satisfaction, have defined the original concept of beauty as splendour in the Serbian language. [Projekat Ministarstva nauke Republike Srbije, br. III 47016: Interdisciplinarno istraživanje kulturnog i jezičkog nasleđa Srbije; Izrada multimedijalnog internet portala Pojmovnik srpske kulture

  6. The Performance Analysis of Smartphone-Based Pedestrian Dead Reckoning and Wireless Locating Technology for Indoor Navigation Application

    Directory of Open Access Journals (Sweden)

    Jhen-Kai Liao

    2016-12-01

    Full Text Available Recent developments in smartphone technology have increased user demand for indoors applications. The Global Navigation Satellite System (GNSS and the Inertial Navigation System (INS are the two advanced systems for navigation technology. However, it is still difficult for GNSS to provide an accurate and practical navigation solution, especially in environments with little or no signal availability. These failings should be easy to overcome; because of their portability and multiple embedded hardware sensors, smartphones seem well positioned to make pedestrian navigation easy and convenient in any environment. Pedestrian Dead Reckoning (PDR is one of the most commonly used technologies used for pedestrian navigation, but it is not always accurate because its positioning errors tend to accumulate over time. Therefore, this research introduces a new tool to overcome this failing; a Bluetooth Low-Energy (BLE beacon can maintain and improve the accuracy of PDR. Moreover, a BLE beacon can be initialized from any user position in an indoor environment. The random and unpredictable positions of pedestrians inevitably result in the degradation of navigation guidance systems’ data. To rectify this problem, we have implemented activity recognition technology to notify the proposed system so as to provide a more accurate heading estimate. This study proposes a Personal Navigation System (PNS based on this technology; it can estimate navigation solutions in real time and combines the advantages of PDR and Bluetooth positioning technology. A series of experiments were conducted to evaluate the accuracy of the system and the efficacy of our proposed algorithms. Preliminary results show the average relative precision of PDR to be about 2.5%, when using a mobile hand-held device. The error of initial position from 2-D beacon positioning is less than two meters. The proposed system works well without post-processing, and the multi-sensor activity recognition

  7. Real-time polymerase chain reaction monitoring of recombinant DNA entry into soil from decomposing roundup ready leaf biomass.

    Science.gov (United States)

    Levy-Booth, David J; Campbell, Rachel G; Gulden, Robert H; Hart, Miranda M; Powell, Jeff R; Klironomos, John N; Pauls, K Peter; Swanton, Clarence J; Trevors, Jack T; Dunfield, Kari E

    2008-08-13

    Glyphosate-tolerant, Roundup Ready (RR) soybeans account for about 57% of all genetically modified (GM) crops grown worldwide. The entry of recombinant DNA into soil from GM crops has been identified as an environmental concern due to the possibility of their horizontal transfer to soil microorganisms. RR soybeans contain recombinant gene sequences that can be differentiated from wild-type plant and microbial genes in soil by using a sequence-specific molecular beacon and real-time polymerase chain reaction (PCR). A molecular beacon-based real-time PCR system to quantify a wild-type soybean lectin ( le1) gene was designed to compare amounts of endogenous soybean genes to recombinant DNA in soil. Microcosm studies were carried out to develop methodologies for the detection of recombinant DNA from RR soybeans in soil. RR soybean leaf litterbags were imbedded in the soil under controlled environmental conditions (60% water holding capacity, 10/15 degrees C, and 8/16 h day/night) for 30 days. The soybean biomass decomposition was described using a single-phase exponential equation, and the DNA concentration in planta and in soil was quantified using real-time PCR using sequence-specific molecular beacons for the recombinant cp4 epsps and endogenous soybean lectin ( le1) genes. The biomass of RR soybean leaves was 8.6% less than nontransgenic (NT) soybean leaves after 30 days. The pooled half-disappearance time for cp4 epsps and le1 in RR and of le1 in NT soybean leaves was 1.4 days. All genes from leaves were detected in soil after 30 days. This study provides a methodology for monitoring the entry of RR and NT soybean DNA into soil from decomposing plant residues.

  8. Bardoxolone Methyl Improves Kidney Function in Patients with Chronic Kidney Disease Stage 4 and Type 2 Diabetes: Post-Hoc Analyses from Bardoxolone Methyl Evaluation in Patients with Chronic Kidney Disease and Type 2 Diabetes Study

    Science.gov (United States)

    Chin, Melanie P.; Bakris, George L.; Block, Geoffrey A.; Chertow, Glenn M.; Goldsberry, Angie; Inker, Lesley A.; Heerspink, Hiddo J.L.; O'Grady, Megan; Pergola, Pablo E.; Wanner, Christoph; Warnock, David G.; Meyer, Colin J.

    2018-01-01

    Background Increases in measured inulin clearance, measured creatinine clearance, and estimated glomerular filtration rate (eGFR) have been observed with bardoxolone methyl in 7 studies enrolling approximately 2,600 patients with type 2 diabetes (T2D) and chronic kidney disease (CKD). The largest of these studies was Bardoxolone Methyl Evaluation in Patients with Chronic Kidney Disease and Type 2 Diabetes (BEACON), a multinational, randomized, double-blind, placebo-controlled phase 3 trial which enrolled patients with T2D and CKD stage 4. The BEACON trial was terminated after preliminary analyses showed that patients randomized to bardoxolone methyl experienced significantly higher rates of heart failure events. We performed post-hoc analyses to characterize changes in kidney function induced by bardoxolone methyl. Methods Patients in ­BEACON (n = 2,185) were randomized 1: 1 to receive once-daily bardoxolone methyl (20 mg) or placebo. We compared the effects of bardoxolone methyl and placebo on a post-hoc composite renal endpoint consisting of ≥30% decline from baseline in eGFR, eGFR <15 mL/min/1.73 m2, and end-stage renal disease (ESRD) events (provision of dialysis or kidney transplantation). Results Consistent with prior studies, patients randomized to bardoxolone methyl experienced mean increases in eGFR that were sustained through study week 48. Moreover, increases in eGFR from baseline were sustained 4 weeks after cessation of treatment. Patients randomized to bardoxolone methyl were significantly less likely to experience the composite renal endpoint (hazards ratio 0.48 [95% CI 0.36–0.64]; p < 0.0001). Conclusions Bardoxolone methyl preserves kidney function and may delay the onset of ESRD in patients with T2D and stage 4 CKD. PMID:29402767

  9. Near-infrared organic materials and emerging applications

    CERN Document Server

    Wang, Zhi Yuan

    2013-01-01

    Highlighting emerging applications of near-infrared (NIR) organic materials that are currently receiving great attention due to their potential use in optical communications, biomedicine, and camouflage materials, this cutting-edge book reviews important recent advances in an accessible style suitable for researchers and graduates in the field on organic/polymer solar cells, optical communications, and advanced optoelectronics. A beacon in the field literature, this comprehensive work discusses several areas of research and development including thermal control and emission detectors in which

  10. Wave propagation and earth satellite radio emission studies

    Science.gov (United States)

    Yeh, K. C.; Liu, C. H.; Flaherty, B. J.

    1974-01-01

    Radio propagation studies of the ionosphere using satellite radio beacons are described. The ionosphere is known as a dispersive, inhomogeneous, irregular and sometimes even nonlinear medium. After traversing through the ionosphere the radio signal bears signatures of these characteristics. A study of these signatures will be helpful in two areas: (1) It will assist in learning the behavior of the medium, in this case the ionosphere. (2) It will provide information of the kind of signal characteristics and statistics to be expected for communication and navigational satellite systems that use the similar geometry.

  11. ns-2 extension to simulate localization system in wireless sensor networks

    CSIR Research Space (South Africa)

    Abu-Mahfouz, Adnan M

    2011-09-01

    Full Text Available of references and termination criterion. As shown in Fig. 2, the Position class uses the MMSE and LocDisApp classes and the ReferenceNode structure, which consists of two members, location variable and double variables to store the measured distance..., such as the routing protocol, MAC type and so on. In addition to these configurations it is also required to specify the attribute of the nodes (either BEACON or UNKNOWN), which can be done with the help of the command ?node-config?. For example, the following...

  12. Spacecraft navigation at Mars using earth-based and in situ radio tracking techniques

    Science.gov (United States)

    Thurman, S. W.; Edwards, C. D.; Kahn, R. D.; Vijayaraghavan, A.; Hastrup, R. C.; Cesarone, R. J.

    1992-08-01

    A survey of earth-based and in situ radiometric data types and results from a number of studies investigating potential radio navigation performance for spacecraft approaching/orbiting Mars and for landed spacecraft and rovers on the surface of Mars are presented. The performance of Doppler, ranging and interferometry earth-based data types involving single or multiple spacecraft is addressed. This evaluation is conducted with that of in situ data types, such as Doppler and ranging measurements between two spacecraft near Mars, or between a spacecraft and one or more surface radio beacons.

  13. smartRestaurant

    OpenAIRE

    Rubio Ramírez de Arellano, Ana Belén

    2016-01-01

    El proyecto smartRestaurant consiste en una aplicación inteligente que trata de ayudar a las personas a perder el mínimo tiempo para reservar mesa en su restaurante habitual. Para ello se ha creado una aplicación que interactúa con beacons colocados en un edificio al que acceden bastantes personas continuamente. A una hora concreta, los usuarios que dispongan de la aplicación smartRestaurant instalada en el móvil recibirán una notificación push con información del restaurante. Una vez reci...

  14. Coal potential of Antartica

    Energy Technology Data Exchange (ETDEWEB)

    Rose, G.; McElroy, C.T.

    1987-01-01

    This report attempts to bring together available information on the coal deposits of Antarctica and discuss factors that would be involved if these deposits were to be explored and mined. Most of the reported principal coal deposits in Antarctica lie generally within the Transantarctic Mountains: the majority are of Permian age and are present in the Victoria Group of the Beacon Supergroup. Several other deposits have been recorded in East Antarctica and in the Antarctic Peninsula, including minor occurrences of Mesozoic and Tertiary coal and carbonaceous shale.

  15. The Speed Control of Constant Tension Motor of Marine Crane

    Directory of Open Access Journals (Sweden)

    Chen Xinyang

    2016-01-01

    Full Text Available This article describes the working principle of the marine beacon crane hanging disc mechanical anti-sway device, and establish mathematical model on the rope controlling hanging disc of mechanical anti-sway device; Through matlab simulation analysis, this article obtains the relation curve between the velocity of traction rope of hanging disc and output frequency of the crane motor, combining rotary crane scaled model, this article carries out anti-sway experiment for the rotary crane to examine the crane’s anti-sway effects.

  16. The planktonic communities of the Jamaican south-east coast; a comparison of harbor, shelf and oceanic areas

    Directory of Open Access Journals (Sweden)

    Hugh Small

    2014-09-01

    Full Text Available Few studies have compared water quality and plankton along the eutrophication gradient from Kingston Harbour to oceanic waters around Jamaica. To compare the planktonic community along the expected nutrient gradient, we sampled every two weeks at four stations, from eutrophic Kingston Harbour to oceanic California Bank. Phytoplankton was assessed from whole water Niskin bottle casts and zooplankton by vertical hauls with plankton nets of three different mesh sizes: 64µm, 200µm, and 600µm. Total phytoplankton biomass declined sharply away from the harbour (1.0 μg L-1 at the Harbour Shoal Beacon to 0.2 μg L-1 at California Bank. Characteristic estuarine phytoplankton genera -such as Ceratium, Gonyaulax, Gyrodinium and Rhizosolenia- dominated harbour samples while genera characteristic of offshore locations -such as Asterionelliopsis, Navicula, Nitzschia, Rhizosolenia and Thalassionema- dominated California Bank. Highest phytoplankton densities (mean values of 34 174 cells L-1 were found at the harbor mouth. Mean zooplankton abundances ranged from maximum (5 858.5m-3 at Beacon to minimum (2 124.2 m-3 at California; 171 species of zooplankton were identified and copepods dominated with 76 species. Overall, 75 species of zooplankton were identified from Beacon, 107 from Port Royal Cays- South East Cay, 110 from the exposed shelf edge- Windward Edge, and 95 from the oceanic California Bank. Larval forms dominated; copepod nauplii, fish eggs and echinoderm larvae occurred at all sites. Lucifer faxoni and Penilia avirostris were indicative of harbor waters and Microsetella sp. and Farranula carinata of offshore waters. Some zooplankton taxa, like L. faxoni, Paracalanus parvus and copepod nauplii, despite showing gradual decline with distance from Beacon to the Edge, increased in abundance at the furthest station, California. California Bank clearly experiences enrichment which at times can be as high as near-shore areas, but the planktonic

  17. Small radioisotope powered batteries

    International Nuclear Information System (INIS)

    Myatt, J.

    1975-06-01

    Various methods of converting the large amounts of energy stored in radioisotopes are described. These are based on:- (a) the Seebeck effect; (b) thermionic emission of electrons from a hot body; (c) the Stirling Cycle; and (d) radiovoltaic charge separation in 'p-n' junctions. Small generators in the range 0 to 100 W(e) developed using these effects are described and typical applications for each of these systems are given. These include data collection and transmission from remote sites, implantable medical devices, lighthouses, radio beacons, and space power supplies. (author)

  18. Devastating tsunami inspires efforts to reduce future tsunami destruction

    Science.gov (United States)

    Lockridge, P.A.

    1987-01-01

    The beacon from landmark Scotch Cap lighthouse pierced the moonless night of April 1, 1946, in Alaska's remote Aleutain Island chain. In the reinforced concrete lighthouse, five men were engaged in various support operations connected with the maintenance of the 80,000 candlepower beam. Perched atop a building constructed 5 years earlier on a bluff 32 feet above sea level, the proud new light rose a total of 92 feet above the swirling restless seas. On a cliff behind the lighthouse, a second building housed the Coast Guard radio-direction-finding station. 

  19. Evaluation of Deep-Space Laser Communication under Different Mission Scenarios

    Science.gov (United States)

    Biswas, Abhijit; Piazzolla, Sabino; Moision, Bruce; Lisman, Douglas

    2012-01-01

    A number of space agencies, including NASA, are considering free-space laser communications as a means for returning higher data-rates from future space missions. In this paper, potential deep-space missions are evaluated to show that with optical communication a 10x increase relative to state-of-the art telecommunication systems could be achieved. The maximum deep-space distance where ground transmitted laser beacons could assist acquisition and tracking; and operating points where optical communication performance degrades faster than the inverse square distance are also discussed.

  20. Atmospheric-Fade-Tolerant Tracking and Pointing in Wireless Optical Communication

    Science.gov (United States)

    Ortiz, Gerardo; Lee, Shinhak

    2003-01-01

    An acquisition, tracking, and pointing (ATP) system, under development at the time of reporting the information for this article, is intended to enable a terminal in a free-space optical communication system to continue to aim its transmitting laser beam toward a receiver at a remote terminal when the laser beacon signal from the remote terminal temporarily fades or drops out of sight altogether. Such fades and dropouts can be caused by adverse atmospheric conditions (e.g., rain or clouds). They can also occur when intervening objects block the line of sight between terminals as a result of motions of those objects or of either or both terminals

  1. A step ahead: strategies for excellence in critical care nursing practice.

    Science.gov (United States)

    Albano, Amy; Elliott, Sheila; Lusardi, Paula; Scott, Susan; Thomas, Diane

    2005-06-01

    The adult intensive care unit (ICU) at Baystate Medical Center is a 24-bed medical-surgical-trauma ICU that provides high-quality care to critically ill patients. Collaboration and expertise among the nursing staff, intensivists, and interdisciplinary colleagues have contributed to its development into a Beacon Award-winning unit. Its primary goal is reflected in the unit's mission: "Care for our patients is guided by knowledge, motivated by compassion, and performed in collaboration with others." Common interests, values, and purposes have created an environment of communication that supports the delivery of exceptional critical care to patients and their families.

  2. A study of the transmitting power control for earth stations

    Science.gov (United States)

    Seta, Mitsuru; Ayukawa, Ichiro

    Various transmitting power control (TPC) methods, particularly for digital data communication systems, are described. Four types of TPC systems are addressed: independent control system, central control system, central pilot system, and pair control system. The detection of rain attenuation and the measurement of signal quality in these systems is examined. Effective TPC methods for three kinds of networks are discussed. For a TDMA network, a central control system with BER detection is effective. A central control system with beacon and pilot is shown to be useful for a very small aperture terminal (VSAT) network, particularly in the Ku-band, and an independent control system is suitable for an SCPC network.

  3. Real-time PCR in Food Science: Introduction.

    Science.gov (United States)

    Rodriguez-Lazaro, David; Hernandez, Marta

    2013-01-01

    Food safety and quality control programmes are increasingly applied throughout the production food chain in order to guarantee added value products as well as to minimize the risk of infection for the consumer. The development of real-time PCR has represented one of the most significant advances in food diagnostics as it provides rapid, reliable and quantitative results. These aspects become increasingly important for the agricultural and food industry. Different strategies for real-time PCR diagnostics have been developed including unspecific detection independent of the target sequence using fluorescent dyes such as SYBR Green, or by sequence-specific fluorescent oligonucleotide probes such as TaqMan probes or molecular beacons.

  4. Radio Resource Management for V2V Discovery

    DEFF Research Database (Denmark)

    Alvarez, Beatriz Soret; Gatnau, Marta; Kovács, Istvan

    2016-01-01

    of each other; and the communication phase itself, where data exchange takes place. In the case of V2V, the discovery phase can utilize the status information that cars broadcast periodically as the beacons to detect the presence of neighbouring cars. For the delivery of specific messages (e.......g., a hazardous event), direct communications can then be set up in a very fast way, based on the previously collected information. Several aspects have been revealed as essential to ensure the reliability and latency requirements of the discovery, such as the autonomous selection of the resources, the duplexing...

  5. Interstellar Abundances Toward X Per, Revisited

    Science.gov (United States)

    Valencic, Lynne A.; Smith, Randall K.

    2014-01-01

    The nearby X-ray binary X Per (HD 24534) provides a useful beacon with which to measure elemental abundances in the local ISM. We examine absorption features of 0, Mg, and Si along this line of sight using spectra from the Chandra Observatory's LETG/ ACIS-S and XMM-Newton's RGS instruments. In general, we find that the abundances and their ratios are similar to those of young F and G stars and the most recent solar values. We compare our results with abundances required by dust grain models.

  6. Investing wisely for longer-term gains.

    Science.gov (United States)

    Baillie, Jonathan

    2011-03-01

    At a time when, with less capital funding available for purchasing high value hospital equipment, NHS board-level and financial personnel may be tempted to sign off purchase of equipment that, while meeting minimum HTM and other standards, may offer compromised performance, especially over time, two key sales and product personnel at leading medical gas equipment supplier, BeaconMedaes, told HEJ editor Jonathan Baillie that "investing a little more" initially would reap considerable longer-term dividends, in payback, lifespan, and patient safety terms.

  7. A Fluorescence Light-Up Ag Nanocluster Probe that Discriminates Single-Nucleotide Variants by Emission Color

    OpenAIRE

    Yeh, Hsin-Chih; Sharma, Jaswinder; Shih, Ie-Ming; Vu, Dung M.; Martinez, Jennifer S.; Werner, James H.

    2012-01-01

    Rapid and precise screening of small genetic variations, such as single-nucleotide polymorphisms (SNPs), among an individual’s genome is still an unmet challenge at point-of-care settings. One crucial step towards this goal is the development of discrimination probes that require no enzymatic reaction and are easy to use. Here we report a new type of fluorescent molecular probe, termed a chameleon NanoCluster Beacon (cNCB), that lights up into different colors upon binding SNP targets. NanoCl...

  8. New approaches to some methodological problems of meteor science

    International Nuclear Information System (INIS)

    Meisel, D.D.

    1987-01-01

    Several low cost approaches to continuous radioscatter monitoring of the incoming meteor flux are described. Preliminary experiments were attempted using standard time frequency stations WWVH and CHU (on frequencies near 15 MHz) during nighttime hours. Around-the-clock monitoring using the international standard aeronautical beacon frequency of 75 MHz was also attempted. The techniques are simple and can be managed routinely by amateur astronomers with relatively little technical expertise. Time series analysis can now be performed using relatively inexpensive microcomputers. Several algorithmic approaches to the analysis of meteor rates are discussed. Methods of obtaining optimal filter predictions of future meteor flux are also discussed

  9. Proceedings of the Annual Symposium on Frequency Control (39th) Held in Philadelphia, Pennsylvania on 29-31 May 1985

    Science.gov (United States)

    1985-05-01

    IM-34, pp. 151-154, June 1985. [81 P. Tremblay, Etude de 1’effet du filtrage sur la caracterisation de la stabilite de la frequance. Ph. D. Thesis...satellite is also requi- requires an orbit determination error less than 10cm. red. The oscillator used in the DORIS receiver must The main topic of the...beacon has a coverage area lower than 1.10 in 10 years. about 200. CEPE has manufactured various prototypes of such The accuracy in orbit

  10. Back to the Future of Dental Education

    Science.gov (United States)

    Reynolds, Patricia A

    2014-01-01

    Our students are like beacons, a bright light on the horizon, but with some way to go. Our educational methods that bring them safely into harbour have seen the test of time, but do we allow them enough freedom and creativity to sail their boats in the climate change of the digital era? Our students are chosen because they are smart and capable and we hope will bring credit to our institutions, but do we maximize their potential educationally? We need to go back to the future to begin to consider this. PMID:27688345

  11. Adaptive optics confocal microscopy using direct wavefront sensing.

    Science.gov (United States)

    Tao, Xiaodong; Fernandez, Bautista; Azucena, Oscar; Fu, Min; Garcia, Denise; Zuo, Yi; Chen, Diana C; Kubby, Joel

    2011-04-01

    Optical aberrations due to the inhomogeneous refractive index of tissue degrade the resolution and brightness of images in deep-tissue imaging. We introduce a confocal fluorescence microscope with adaptive optics, which can correct aberrations based on direct wavefront measurements using a Shack-Hartmann wavefront sensor with a fluorescent bead used as a point source reference beacon. The results show a 4.3× improvement in the Strehl ratio and a 240% improvement in the signal intensity for fixed mouse tissues at depths of up to 100 μm.

  12. High Efficiency Power Converter for Low Voltage High Power Applications

    DEFF Research Database (Denmark)

    Nymand, Morten

    The topic of this thesis is the design of high efficiency power electronic dc-to-dc converters for high-power, low-input-voltage to high-output-voltage applications. These converters are increasingly required for emerging sustainable energy systems such as fuel cell, battery or photo voltaic based......, and remote power generation for light towers, camper vans, boats, beacons, and buoys etc. A review of current state-of-the-art is presented. The best performing converters achieve moderately high peak efficiencies at high input voltage and medium power level. However, system dimensioning and cost are often...

  13. Structural Discrimination and Autonomous Vehicles

    DEFF Research Database (Denmark)

    Liu, Hin-Yan

    2016-01-01

    This paper examines the potential for structural discrimination to be woven into the fabric of autonomous vehicle developments, which remain underexplored and undiscussed. The prospect for structural discrimination arises as a result of the coordinated modes of autonomous vehicle behaviour...... discrimination looms with the possibility of crash optimisation impulses in which a protective shield is cast over those individuals in which society may have a vested interest in prioritising or safeguarding. A stark dystopian scenario is introduced to sketch the contours whereby personal beacons signal...

  14. Survey of Technology with Possible Applications to United States Coast Guard Buoy Tenders. Volume 2. Literature Abstracts.

    Science.gov (United States)

    1987-09-01

    34THV MERMAID ." Vessel particular: LOA - 80.4 m, Beam moulded - 14.5 m, Draft - 4.0 m, speed 12 kts, complement - 24, power - diesel electric 2x 860 kW...Beacons. Included in this yearbook is a 2-page spread with particulars on the newest buoy tender "THV MERMAID " recently completed by Hyundai Heavy...suppliers. A little short on detailed design information. Covers all self-propelled OSV’s over 60ft LOA except for pure tugs. Patton, L.M., "The

  15. WLAN-verkon optimointi kotikäyttöön

    OpenAIRE

    Hyökki, Jyri

    2016-01-01

    Opinnäytetyön tavoitteena oli löytää keinoja langattoman lähiverkon (WLAN) suorituskyvyn parantamiseksi kotikäyttöön ja usean käyttäjän WLAN-verkkoon. Opinnäytetyössä esitettyjen tutkimusten ja testien myötä DNA Oy toteuttaa tarvittaessa muutoksia kuluttajatason WLAN-laitteisiin. WLAN-verkkoa tarjoava tukiasema tai reititin sisältää paljon suorituskykyyn vaikuttavia asetuksia, joita ovat kanavan leveyden valinta, kanavan valinta, Airtime Fairness, Band Steering, Beamforming, Beacon Interv...

  16. Deep space optical communication via relay satellite

    Science.gov (United States)

    Dolinar, S.; Vilnrotter, V.; Gagliardi, R.

    1981-01-01

    The application of optical communications for a deep space link via an earth-orbiting relay satellite is discussed. The system uses optical frequencies for the free-space channel and RF links for atmospheric transmission. The relay satellite is in geostationary orbit and contains the optics necessary for data processing and formatting. It returns the data to earth through the RF terrestrial link and also transmits an optical beacon to the satellite for spacecraft return pointing and for the alignment of the transmitting optics. Future work will turn to modulation and coding, pointing and tracking, and optical-RF interfacing.

  17. Cytoskeleton and Cytoskeleton-Bound RNA Visualization in Frog and Insect Oocytes.

    Science.gov (United States)

    Kloc, Malgorzata; Bilinski, Szczepan; Kubiak, Jacek Z

    2016-01-01

    The majority of oocyte functions involves and depends on the cytoskeletal elements, which include microtubules and actin and cytokeratin filaments. Various structures and molecules are temporarily or permanently bound to the cytoskeletal elements and their functions rely on cytoskeleton integrity and its timely assembly. Thus the accurate visualization of cytoskeleton is often crucial for studies and analyses of oocyte structure and functions. Here we describe several reliable methods for microtubule and/or microfilaments preservation and visualization in Xenopus oocyte extracts, and in situ in live and fixed insect and frog (Xenopus) oocytes. In addition, we describe visualization of cytoskeleton-bound RNAs using molecular beacons in live Xenopus oocytes.

  18. The Sytar network: a radioactivity observatory

    International Nuclear Information System (INIS)

    Robeau, D.; Calmet, D.

    1992-01-01

    A Radiological Transmission and Early Warning System (SYTAR) connects a network of stations all over France to the Emergency Centre of the Institut de Protection et de Surete Nucleaire in Fontenay aux Roses. Under normal circumstances, radiological data collection is done in three different ways, (i) a real time transmission of total alpha and beta in aerosols and external gamma radiation measurements performed and relayed by beacons fitted with sequential filters; (ii) a transmission of recorded data containing results of alpha spectrometry: weekly beta and gamma measurements of biological indicators, (iii) in accident conditions the rate of data delivery is stepped up

  19. Network Signaling Channel for Improving ZigBee Performance in Dynamic Cluster-Tree Networks

    Directory of Open Access Journals (Sweden)

    D. Hämäläinen

    2008-03-01

    Full Text Available ZigBee is one of the most potential standardized technologies for wireless sensor networks (WSNs. Yet, sufficient energy-efficiency for the lowest power WSNs is achieved only in rather static networks. This severely limits the applicability of ZigBee in outdoor and mobile applications, where operation environment is harsh and link failures are common. This paper proposes a network channel beaconing (NCB algorithm for improving ZigBee performance in dynamic cluster-tree networks. NCB reduces the energy consumption of passive scans by dedicating one frequency channel for network beacon transmissions and by energy optimizing their transmission rate. According to an energy analysis, the power consumption of network maintenance operations reduces by 70%–76% in dynamic networks. In static networks, energy overhead is negligible. Moreover, the service time for data routing increases up to 37%. The performance of NCB is validated by ns-2 simulations. NCB can be implemented as an extension on MAC and NWK layers and it is fully compatible with ZigBee.

  20. LSPV+7, a branch-point-tolerant reconstructor for strong turbulence adaptive optics.

    Science.gov (United States)

    Steinbock, Michael J; Hyde, Milo W; Schmidt, Jason D

    2014-06-20

    Optical wave propagation through long paths of extended turbulence presents unique challenges to adaptive optics (AO) systems. As scintillation and branch points develop in the beacon phase, challenges arise in accurately unwrapping the received wavefront and optimizing the reconstructed phase with respect to branch cut placement on a continuous facesheet deformable mirror. Several applications are currently restricted by these capability limits: laser communication, laser weapons, remote sensing, and ground-based astronomy. This paper presents a set of temporally evolving AO simulations comparing traditional least-squares reconstruction techniques to a complex-exponential reconstructor and several other reconstructors derived from the postprocessing congruence operation. The reconstructors' behavior in closed-loop operation is compared and discussed, providing several insights into the fundamental strengths and limitations of each reconstructor type. This research utilizes a self-referencing interferometer (SRI) as the high-order wavefront sensor, driving a traditional linear control law in conjunction with a cooperative point source beacon. The SRI model includes practical optical considerations and frame-by-frame fiber coupling effects to allow for realistic noise modeling. The "LSPV+7" reconstructor is shown to offer the best performance in terms of Strehl ratio and correction stability-outperforming the traditional least-squares reconstructed system by an average of 120% in the studied scenarios. Utilizing a continuous facesheet deformable mirror, these reconstructors offer significant AO performance improvements in strong turbulence applications without the need for segmented deformable mirrors.

  1. More rain compensation results

    Science.gov (United States)

    Sworder, D. D.; Vojak, R.

    1992-01-01

    To reduce the impact of rain-induced attenuation in the 20/30 GHz band, the attenuation at a specified signal frequency must be estimated and extrapolated forward in time on the basis of a noisy beacon measurement. Several studies have used model based procedures for solving this problem in statistical inference. Perhaps the most widely used model-based paradigm leads to the Kalman filter and its lineal variants. In this formulation, the dynamic features of the attenuation are represented by a state process (x(sub t)). The observation process (y(sub t)) is derived from beacon measurements. Some ideas relating to the signal processing problems related to uplink power control are presented. It is shown that some easily implemented algorithms hold promise for use in estimating rain induced fades. The algorithms were applied to actual data generated at the Virginia Polytechnic Institute and State University (VPI) test facility. Because only one such event was studied, it is not clear that the algorithms will have the same effectiveness when a wide range of events are studied.

  2. APPROACH AND EVALUATION OF A MOBILE VIDEO-BASED AND LOCATION-BASED AUGMENTED REALITY PLATFORM FOR INFORMATION BROKERAGE

    Directory of Open Access Journals (Sweden)

    H. Dastageeri

    2016-09-01

    Full Text Available Providing mobile location-based information for pedestrians faces many challenges. On one hand the accuracy of localisation indoors and outdoors is restricted due to technical limitations of GPS and Beacons. Then again only a small display is available to display information as well as to develop a user interface. Plus, the software solution has to consider the hardware characteristics of mobile devices during the implementation process for aiming a performance with minimum latency. This paper describes our approach by including a combination of image tracking and GPS or Beacons to ensure orientation and precision of localisation. To communicate the information on Points of Interest (POIs, we decided to choose Augmented Reality (AR. For this concept of operations, we used besides the display also the acceleration and positions sensors as a user interface. This paper especially goes into detail on the optimization of the image tracking algorithms, the development of the video-based AR player for the Android platform and the evaluation of videos as an AR element in consideration of providing a good user experience. For setting up content for the POIs or even generate a tour we used and extended the Open Geospatial Consortium (OGC standard Augmented Reality Markup Language (ARML.

  3. The Combined Radio Interferometry and COSMIC Experiment in Tomography (CRICKET) Campaign

    Science.gov (United States)

    Dymond, Kenneth; Coker, Clayton; Bernhardt, Paul; Cohen, Aaron; Crane, Patrick; Kassim, Namir; Lazio, Joseph; Weiler, Kurt; Watts, Christopher; Rickard, Lee J.; Taylor, Greg; Schinzel, Frank; Philstrom, Ylva; Close, Sigrid; Colestock, Patrick; Myers, Steve; Datta, Abirhup

    We report on the Combined Radio Interferometry and COSMIC Experiment in Tomography Campaign (CRICKET) held on September 15 and 17, 2007. The experiment used the Constellation Observing System for Meteorology, Ionosphere, and Climate (COSMIC also known as FORMOSAT-3) in conjunction with the Very Large Array radio telescope, located near Socorro, NM, to study the ionosphere from the global scale down to the regional scale. Each COSMIC satellite includes three instruments capable of measuring the ionosphere: the Tiny Ionospheric Photometer (TIP), a UV radiometer; the GPS Occultation experiment (GOX), a dual-frequency GPS occultation receiver; and the Tri-band Beacon (TBB), a three frequency coherently radiating radio beacon. These three instruments have been demonstrated to be a powerful means for characterizing the global-scale ionosphere. The VLA when deployed at its largest extent and while operating at 73.8 MHz is incredibly sensitive to relative total electron content variations of the regional ionosphere over about a 30-100 km diameter area. In this work, we concentrate on the first set of observations on September 15, 2007 at approximately 0830 UT. We have successfully married these heterogeneous data sets, using a tomographic data fusion approach, to produce a consistent ionospheric specification from the global scale down to the regional scale.

  4. Status of the ARGOS project

    Science.gov (United States)

    Rabien, Sebastian; Barl, Lothar; Beckmann, Udo; Bonaglia, Marco; Borelli, José Luis; Brynnel, Joar; Buschkamp, Peter; Busoni, Lorenzo; Christou, Julian; Connot, Claus; Davies, Richard; Deysenroth, Matthias; Esposito, Simone; Gässler, Wolfgang; Gemperlein, Hans; Hart, Michael; Kulas, Martin; Lefebvre, Michael; Lehmitz, Michael; Mazzoni, Tommaso; Nussbaum, Edmund; Orban de Xivry, Gilles; Peter, Diethard; Quirrenbach, Andreas; Raab, Walfried; Rahmer, Gustavo; Storm, Jesper; Ziegleder, Julian

    2014-07-01

    ARGOS is the Laser Guide Star and Wavefront sensing facility for the Large Binocular Telescope. With first laser light on sky in 2013, the system is currently undergoing commissioning at the telescope. We present the overall status and design, as well as first results on sky. Aiming for a wide field ground layer correction, ARGOS is designed as a multi- Rayleigh beacon adaptive optics system. A total of six powerful pulsed lasers are creating the laser guide stars in constellations above each of the LBTs primary mirrors. With a range gated detection in the wavefront sensors, and the adaptive correction by the deformable secondary's, we expect ARGOS to enhance the image quality over a large range of seeing conditions. With the two wide field imaging and spectroscopic instruments LUCI1 and LUCI2 as receivers, a wide range of scientific programs will benefit from ARGOS. With an increased resolution, higher encircled energy, both imaging and MOS spectroscopy will be boosted in signal to noise by a large amount. Apart from the wide field correction ARGOS delivers in its ground layer mode, we already foresee the implementation of a hybrid Sodium with Rayleigh beacon combination for a diffraction limited AO performance.

  5. Testing and integrating the laser system of ARGOS: the ground layer adaptive optics for LBT

    Science.gov (United States)

    Loose, C.; Rabien, S.; Barl, L.; Borelli, J.; Deysenroth, M.; Gaessler, W.; Gemperlein, H.; Honsberg, M.; Kulas, M.; Lederer, R.; Raab, W.; Rahmer, G.; Ziegleder, J.

    2012-07-01

    The Laser Guide Star facility ARGOS will provide Ground Layer Adaptive Optics to the Large Binocular Telescope (LBT). The system operates three pulsed laser beacons above each of the two primary mirrors, which are Rayleigh scattered in 12km height. This enables correction over a wide field of view, using the adaptive secondary mirror of the LBT. The ARGOS laser system is designed around commercially available, pulsed Nd:YAG lasers working at 532 nm. In preparation for a successful commissioning, it is important to ascertain that the specifications are met for every component of the laser system. The testing of assembled, optical subsystems is likewise necessary. In particular it is required to confirm a high output power, beam quality and pulse stability of the beacons. In a second step, the integrated laser system along with its electronic cabinets are installed on a telescope simulator. This unit is capable of carrying the whole assembly and can be tilted to imitate working conditions at the LBT. It allows alignment and functionality testing of the entire system, ensuring that flexure compensation and system diagnosis work properly in different orientations.

  6. Integration and laboratory characterization of the ARGOS laser guide star wavefront sensors

    Science.gov (United States)

    Busoni, Lorenzo; Bonaglia, Marco; Carbonaro, Luca; Mazzoni, Tommaso; Antichi, Jacopo; Esposito, Simone; Orban De Xivry, Gilles; Rabien, Sebastian

    2013-12-01

    The integration status of the ARGOS wavefront sensors is presented. ARGOS is the laser guide star AO program for the LBT. It will implement a Ground Layer AO correction for the instruments LUCI, an infrared imaging and spectrograph camera, using 3 pulsed low-altitudes Rayleigh beacons for each LBT's eye. It profits of the LBT's adaptive secondary mirrors and of FLAO's pyramid unit for NGS sensing. Each LGS is independently stabilized for on-sky jitter and range-gated using custom Pockels cells and then sensed by a 15x15 SH sensor. The 3 pupil images are reimaged on a single lenslet array and a single detector. In the WFS are also installed 3 patrol cameras for the acquisition of the laser beacons, a system for the stabilization of the pupil images on the lenslet array and an internal source for calibration purposes. The two units are now completing the integration phase in Arcetri premises. We describe the characterization of the units and the closed-loop test realized using a deformable MEMS mirror.

  7. A fluorescence-quenching platform based on biomineralized hydroxyapatite from natural seashell and applied to cancer cell detection.

    Science.gov (United States)

    Zhang, Ying; Liu, Wei; Banks, Craig E; Liu, Fei; Li, Mao; Xia, Fan; Yang, Xiangliang

    2014-12-19

    As a typical biomineral, hydroxyapatite (HAp) is widely applied in bone implants and other related fields. However, the inherent nature of HAp can potentially be altered through restricting its fabrication conditions. Here, HAp fabricated by a hydrothermal treatment of pieces of natural seashell is demonstrated to have the capability of fluorescence quenching. To the best of the author's knowledge, this is the first time that this new property of HAp has been reported. Consequently, we assembled a fluorescence-quenching platform based on the biomineralized HAp substrate following a hydrothermal treatment and associated with a DNA molecular beacon and applied to cancer cell detection by the transformation from "OFF state" (fluorescence quenching) to "ON state" (fluorescence recovery). Herein, we found that the outer surface of HAp material after hydrothermal biomineralization for 5 days has considerable capability for both fluorescence quenching and recovery. These results may also have implications in the further detection of various targets such as cancer cells with other special surface antigens, significant biological small molecules or disease related microRNA, just by changing the sequence of the nucleic acid beacon according to the corresponding aptamer.

  8. Micro-machined resonator oscillator

    Science.gov (United States)

    Koehler, Dale R.; Sniegowski, Jeffry J.; Bivens, Hugh M.; Wessendorf, Kurt O.

    1994-01-01

    A micro-miniature resonator-oscillator is disclosed. Due to the miniaturization of the resonator-oscillator, oscillation frequencies of one MHz and higher are utilized. A thickness-mode quartz resonator housed in a micro-machined silicon package and operated as a "telemetered sensor beacon" that is, a digital, self-powered, remote, parameter measuring-transmitter in the FM-band. The resonator design uses trapped energy principles and temperature dependence methodology through crystal orientation control, with operation in the 20-100 MHz range. High volume batch-processing manufacturing is utilized, with package and resonator assembly at the wafer level. Unique design features include squeeze-film damping for robust vibration and shock performance, capacitive coupling through micro-machined diaphragms allowing resonator excitation at the package exterior, circuit integration and extremely small (0.1 in. square) dimensioning. A family of micro-miniature sensor beacons is also disclosed with widespread applications as bio-medical sensors, vehicle status monitors and high-volume animal identification and health sensors. The sensor family allows measurement of temperatures, chemicals, acceleration and pressure. A microphone and clock realization is also available.

  9. Enzyme-free amplification for sensitive electrochemical detection of DNA via target-catalyzed hairpin assembly assisted current change.

    Science.gov (United States)

    Qian, Yong; Wang, Chunyan; Gao, Fenglei

    2014-12-01

    An isothermal, enzyme-free and sensitive method for electrochemical detection of DNA is proposed based on target catalyzed hairpin assembly and for signal amplification. Molecular beacon 1 (MB1) contains a ferrocene (Fc) tag, which was immobilized on the gold electrode as recognition probe to hybridize with target DNA. Then, molecular beacon 2 hybridized with the opened MB1, allowing the target to be displaced. The displaced target again triggered the next round of strand exchange reaction resulting in many Fc far away from the GE to achieve signal amplification for sensitive DNA detection. The current signal amplification strategy is relatively simple and inexpensive owing to avoid the use of any kind of enzyme or sophisticated equipment. It can achieve a sensitivity of 42 fM with a wide linear dynamic range from 10(-13) to 10(-9)M and discriminate mismatched DNA from perfect matched target DNA with a high selectivity. The proposed method showed excellent specificity, high sensitivity and low detection limit, and could be applied in analysis of real samples. Copyright © 2014 Elsevier B.V. All rights reserved.

  10. STARSAT: A Joint NASA/AF Project for Laser Calibration of Small Objects in Space

    Science.gov (United States)

    Campbell, Jonathan W.; Carruth, Ralph; Howell, Joe T. (Technical Monitor)

    2002-01-01

    The Air Force Research Laboratory/Directed Energy Directorate (AFRL/DE) and the NASA/Marshall Space Flight Center are planning a joint laser space calibration experiments using the HI-CLASS ladar system and the Advanced Electro-Optical System (AEOS) located at Maui Space Surveillance System (MSSS). These experiments will use ladar data to provide accurate range and signature measurements of calibration spheres with known sizes. Ancillary experiment benefits could include calibrating operational sensors, analyzing effects of orbital perturbations on micro-satellites, and correlating radar and optical signatures. In the first experiment, a GPS/laser beacon instrumented micro-satellite about 20 cm in diameter would be deployed from a Space Shuttle Hitchhiker canister. Orbiting in LEO, the micro-satellite would pass over MSSS on the average of two times per 24 hour period. A timing circuit would activate the GPS unit that would in turn activate the laser beacon at the appropriate time. The HI-CLASS/AEOS system will detect and acquire the micro-sat as it rises above the horizon and demonstrate that continuous track can be maintained throughout the pass. Orbital parameters will be obtained. This operational approach should maximize visibility to the ground-based laser while allowing battery life to be conserved thus extending the lifetime of the satellite. GPS data will be transmitted to the ground, providing independent location information down to cm accuracies for comparison and evaluation purposes.

  11. Achieving Operational Two-Way Laser Acquisition for OPALS Payload on the International Space Station

    Science.gov (United States)

    Abrahamson, Matthew J.; Oaida, Bogdan V.; Sindiy, Oleg; Biswas, Abhijit

    2015-01-01

    The Optical PAyload for Lasercomm Science (OPALS) experiment was installed on the International Space Station (ISS) in April 2014. Developed as a technology demonstration, its objective was to experiment with space-to-ground optical communications transmissions from Low Earth Orbit. More than a dozen successful optical links were established between a Wrightwood, California-based ground telescope and the OPALS flight terminal from June 2014 to September 2014. Each transmission required precise bi-directional pointing to be maintained between the space-based transmitter and ground-based receiver. This was accomplished by acquiring and tracking a laser beacon signal transmitted from the ground telescope to the OPALS flight terminal on the ISS. OPALS demonstrated the ability to nominally acquire the beacon within three seconds at 25deg elevation and maintain lock within 140 µrad (3(sigma)) for the full 150-second transmission duration while slewing at rates up to 1deg/sec. Additional acquisition attempts in low elevation and weather-challenged conditions provided valuable insight on the optical link robustness under off-nominal operational conditions.

  12. ITRF2014 orbits for CryoSat-2 based on DORIS and SLR tracking

    Science.gov (United States)

    Schrama, Ernst

    2017-04-01

    With the advent of the new reference system ITRF2014 we have modified our precision orbit determination procedures so that all CryoSat-2 trajectories are now realized in the new reference system which provides a consistent survey of DORIS and SLR station coordinates. For SLR stations in ITRF2014 we take the solution from the SINEX files, there is no need to estimate SLR station coordinates. This is also the case for most DORIS beacons although some beacons require us to estimate station positions since they are not in ITRF2014. Preliminary results show that the DORIS residuals are consistent at 0.4 mm/s and that the SLR residuals are around to 1.5 cm. The level of unexplained accelerations with the presently used dynamic models is around 3.6 nanometer per second squared in the flight direction, for traverse track accelerations it is around 10 nm/s2. Crossover residuals of the CryoSat-2 altimeter typically reduce to under 5 cm, an independent comparison to the CNES precision orbit solutions yields radial differences of around 1.5 cm.

  13. Development of extraterrestrial intelligence and physical laws

    Science.gov (United States)

    Troitskij, V. S.

    This paper considers the restrictions imposed by physical laws on the development of life and intelligence in the form of extraterrestrial civilizations. For this purpose intelligence is defined as the community of intelligent beings, joined by the exchange of mass, energy and information both between themselves and with the external medium. Due to the limitation of the velocity of exchange of information and, in particular, mass and energy exchange, the dimensions of the intelligence cannot exceed some light days, i.e. they are limited by the habitable zone about their star. It is shown that the energy consumption should not exceed the energy output of their star for the sake of preserving the cosmic near-star zone of life from energetic pollution. With the above restrictions of the energy product it takes millions of years to create an omnidirectional beacon-transmitter signals from which would be received by the contemporary antennas in all our Galaxy. It is realistic to create an omnidirectional beacon operating in the range of no more than 100-1000 light years.

  14. Comparison of nine different real-time PCR chemistries for qualitative and quantitative applications in GMO detection.

    Science.gov (United States)

    Buh Gasparic, Meti; Tengs, Torstein; La Paz, Jose Luis; Holst-Jensen, Arne; Pla, Maria; Esteve, Teresa; Zel, Jana; Gruden, Kristina

    2010-03-01

    Several techniques have been developed for detection and quantification of genetically modified organisms, but quantitative real-time PCR is by far the most popular approach. Among the most commonly used real-time PCR chemistries are TaqMan probes and SYBR green, but many other detection chemistries have also been developed. Because their performance has never been compared systematically, here we present an extensive evaluation of some promising chemistries: sequence-unspecific DNA labeling dyes (SYBR green), primer-based technologies (AmpliFluor, Plexor, Lux primers), and techniques involving double-labeled probes, comprising hybridization (molecular beacon) and hydrolysis (TaqMan, CPT, LNA, and MGB) probes, based on recently published experimental data. For each of the detection chemistries assays were included targeting selected loci. Real-time PCR chemistries were subsequently compared for their efficiency in PCR amplification and limits of detection and quantification. The overall applicability of the chemistries was evaluated, adding practicability and cost issues to the performance characteristics. None of the chemistries seemed to be significantly better than any other, but certain features favor LNA and MGB technology as good alternatives to TaqMan in quantification assays. SYBR green and molecular beacon assays can perform equally well but may need more optimization prior to use.

  15. Transfer of Real-time Dynamic Radiation Environment Assimilation Model; Research to Operation

    Science.gov (United States)

    Cho, K. S. F.; Hwang, J.; Shin, D. K.; Kim, G. J.; Morley, S.; Henderson, M. G.; Friedel, R. H.; Reeves, G. D.

    2015-12-01

    Real-time Dynamic Radiation Environment Assimilation Model (rtDREAM) was developed by LANL for nowcast of energetic electrons' flux at the radiation belt to quantify potential risks from radiation damage at the satellites. Assimilated data are from multiple sources including LANL assets (GEO, GPS). For transfer from research to operation of the rtDREAM code, LANL/KSWC/NOAA makes a Memorandum Of Understanding (MOU) on the collaboration between three parts. By this MOU, KWSC/RRA provides all the support for transitioning the research version of DREAM to operations. KASI is primarily responsible for providing all the interfaces between the current scientific output formats of the code and useful space weather products that can be used and accessed through the web. In the second phase, KASI will be responsible in performing the work needed to transform the Van Allen Probes beacon data into "DREAM ready" inputs. KASI will also provide the "operational" code framework and additional data preparation, model output, display and web page codes back to LANL and SWPC. KASI is already a NASA partnering ground station for the Van Allen Probes' space weather beacon data and can here show use and utility of these data for comparison between rtDREAM and observations by web. NOAA has offered to take on some of the data processing tasks specific to the GOES data.

  16. Human place and response learning: navigation strategy selection, pupil size and gaze behavior.

    Science.gov (United States)

    de Condappa, Olivier; Wiener, Jan M

    2016-01-01

    In this study, we examined the cognitive processes and ocular behavior associated with on-going navigation strategy choice using a route learning paradigm that distinguishes between three different wayfinding strategies: an allocentric place strategy, and the egocentric associative cue and beacon response strategies. Participants approached intersections of a known route from a variety of directions, and were asked to indicate the direction in which the original route continued. Their responses in a subset of these test trials allowed the assessment of strategy choice over the course of six experimental blocks. The behavioral data revealed an initial maladaptive bias for a beacon response strategy, with shifts in favor of the optimal configuration place strategy occurring over the course of the experiment. Response time analysis suggests that the configuration strategy relied on spatial transformations applied to a viewpoint-dependent spatial representation, rather than direct access to an allocentric representation. Furthermore, pupillary measures reflected the employment of place and response strategies throughout the experiment, with increasing use of the more cognitively demanding configuration strategy associated with increases in pupil dilation. During test trials in which known intersections were approached from different directions, visual attention was directed to the landmark encoded during learning as well as the intended movement direction. Interestingly, the encoded landmark did not differ between the three navigation strategies, which is discussed in the context of initial strategy choice and the parallel acquisition of place and response knowledge.

  17. Beaconless operation for optimal laser beam propagation through turbulent atmosphere

    Science.gov (United States)

    Khizhnyak, Anatoliy; Markov, Vladimir

    2016-09-01

    Corruption of the wavefront, beam wondering and power density degradation at the receiving end are the effects typically observed at laser beam propagation through turbulent atmosphere. Compensation of these effects can be achieved if the reciprocal conditions for the propagating wave are satisfied along the propagation range. Practical realization of these conditions requires placing a localized beacon at the receiving end of the range and high-performance adaptive optics system (AOS). The key condition for an effective performance of AOS is a high value of the reciprocal component in the outgoing wave, since only this component is getting compensated after propagating turbulence perturbed path. The nonreciprocal components that is present in the wave directed toward the target is caused by three factors (detailed in this paper) that determine the partial restoration of the structure of the beacon beam. Thus solution of a complex problem of focusing the laser beam propagating through turbulent media can be achieved for the share of the outgoing wave that has a reciprocal component. This paper examines the ways and means that can be used in achieving the stated goal of effective laser power delivery on the distant image-resolved object.

  18. Towards Optimal Event Detection and Localization in Acyclic Flow Networks

    KAUST Repository

    Agumbe Suresh, Mahima

    2012-01-03

    Acyclic flow networks, present in many infrastructures of national importance (e.g., oil & gas and water distribution systems), have been attracting immense research interest. Existing solutions for detecting and locating attacks against these infrastructures, have been proven costly and imprecise, especially when dealing with large scale distribution systems. In this paper, to the best of our knowledge for the first time, we investigate how mobile sensor networks can be used for optimal event detection and localization in acyclic flow networks. Sensor nodes move along the edges of the network and detect events (i.e., attacks) and proximity to beacon nodes with known placement in the network. We formulate the problem of minimizing the cost of monitoring infrastructure (i.e., minimizing the number of sensor and beacon nodes deployed), while ensuring a degree of sensing coverage in a zone of interest and a required accuracy in locating events. We propose algorithms for solving these problems and demonstrate their effectiveness with results obtained from a high fidelity simulator.

  19. A comparison of gateway in-street sign configuration to other driver prompts to increase yielding to pedestrians at crosswalks.

    Science.gov (United States)

    Bennett, Miles K; Manal, Hana; Van Houten, Ron

    2014-01-01

    Increasing motorists' yielding of the right of way to pedestrians in crosswalks reduces the number of collisions between motorists and pedestrians. In this study we examined a gateway in-street sign configuration (1 in-street sign installed between the 2 travel lanes in each direction, and 1 on both edges of the roadway in each direction) on multilane roads. The first experiment compared the efficacy of adding multiple in-street signs used in a gateway configuration with a single sign between the 2 travel lanes in each direction. The second experiment compared the gateway in-street sign configuration with a more expensive pedestrian hybrid beacon. The third experiment compared the gateway in-street sign configuration with the more expensive rectangular rapid flashing beacon. The results demonstrated that the gateway in-street sign configuration produced very high levels of driver yielding, and that it was as effective as the 2 more expensive treatments. © Society for the Experimental Analysis of Behavior.

  20. VisNAV 100: a robust, compact imaging sensor for enabling autonomous air-to-air refueling of aircraft and unmanned aerial vehicles

    Science.gov (United States)

    Katake, Anup; Choi, Heeyoul

    2010-01-01

    To enable autonomous air-to-refueling of manned and unmanned vehicles a robust high speed relative navigation sensor capable of proving high accuracy 3DOF information in diverse operating conditions is required. To help address this problem, StarVision Technologies Inc. has been developing a compact, high update rate (100Hz), wide field-of-view (90deg) direction and range estimation imaging sensor called VisNAV 100. The sensor is fully autonomous requiring no communication from the tanker aircraft and contains high reliability embedded avionics to provide range, azimuth, elevation (3 degrees of freedom solution 3DOF) and closing speed relative to the tanker aircraft. The sensor is capable of providing 3DOF with an error of 1% in range and 0.1deg in azimuth/elevation up to a range of 30m and 1 deg error in direction for ranges up to 200m at 100Hz update rates. In this paper we will discuss the algorithms that were developed in-house to enable robust beacon pattern detection, outlier rejection and 3DOF estimation in adverse conditions and present the results of several outdoor tests. Results from the long range single beacon detection tests will also be discussed.

  1. Star Tracker Based ATP System Conceptual Design and Pointing Accuracy Estimation

    Science.gov (United States)

    Orfiz, Gerardo G.; Lee, Shinhak

    2006-01-01

    A star tracker based beaconless (a.k.a. non-cooperative beacon) acquisition, tracking and pointing concept for precisely pointing an optical communication beam is presented as an innovative approach to extend the range of high bandwidth (> 100 Mbps) deep space optical communication links throughout the solar system and to remove the need for a ground based high power laser as a beacon source. The basic approach for executing the ATP functions involves the use of stars as the reference sources from which the attitude knowledge is obtained and combined with high bandwidth gyroscopes for propagating the pointing knowledge to the beam pointing mechanism. Details of the conceptual design are presented including selection of an orthogonal telescope configuration and the introduction of an optical metering scheme to reduce misalignment error. Also, estimates are presented that demonstrate that aiming of the communications beam to the Earth based receive terminal can be achieved with a total system pointing accuracy of better than 850 nanoradians (3 sigma) from anywhere in the solar system.

  2. Open Dataset for the Automatic Recognition of Sedentary Behaviors.

    Science.gov (United States)

    Possos, William; Cruz, Robinson; Cerón, Jesús D; López, Diego M; Sierra-Torres, Carlos H

    2017-01-01

    Sedentarism is associated with the development of noncommunicable diseases (NCD) such as cardiovascular diseases (CVD), type 2 diabetes, and cancer. Therefore, the identification of specific sedentary behaviors (TV viewing, sitting at work, driving, relaxing, etc.) is especially relevant for planning personalized prevention programs. To build and evaluate a public a dataset for the automatic recognition (classification) of sedentary behaviors. The dataset included data from 30 subjects, who performed 23 sedentary behaviors while wearing a commercial wearable on the wrist, a smartphone on the hip and another in the thigh. Bluetooth Low Energy (BLE) beacons were used in order to improve the automatic classification of different sedentary behaviors. The study also compared six well know data mining classification techniques in order to identify the more precise method of solving the classification problem of the 23 defined behaviors. A better classification accuracy was obtained using the Random Forest algorithm and when data were collected from the phone on the hip. Furthermore, the use of beacons as a reference for obtaining the symbolic location of the individual improved the precision of the classification.

  3. An Analysis of Multiple Criteria and Setups for Bluetooth Smartphone-Based Indoor Localization Mechanism

    Directory of Open Access Journals (Sweden)

    Manuel Castillo-Cara

    2017-01-01

    Full Text Available Bluetooth Low Energy (BLE 4.0 beacons will play a major role in the deployment of energy-efficient indoor localization mechanisms. Since BLE4.0 is highly sensitive to fast fading impairments, numerous ongoing studies are currently exploring the use of supervised learning algorithm as an alternative approach to exploit the information provided by the indoor radio maps. Despite the large number of results reported in the literature, there are still many open issues on the performance evaluation of such approach. In this paper, we start by identifying, in a simple setup, the main system parameters to be taken into account on the design of BLE4.0 beacons-based indoor localization mechanisms. In order to shed some light on the evaluation process using supervised learning algorithm, we carry out an in-depth experimental evaluation in terms of the mean localization error, local prediction accuracy, and global prediction accuracy. Based on our results, we argue that, in order to fully assess the capabilities of supervised learning algorithms, it is necessary to include all the three metrics.

  4. High resolution SETI: Experiences and prospects

    Science.gov (United States)

    Horowitz, Paul; Clubok, Ken

    Megachannel spectroscopy with sub-Hertz resolution constitutes an attractive strategy for a microwave search for extraterrestrial intelligence (SETI), assuming the transmission of a narrowband radiofrequency beacon. Such resolution matches the properties of the interstellar medium, and the necessary Doppler corrections provide a high degree of interference rejection. We have constructed a frequency-agile receiver with an FFT-based 8 megachannel digital spectrum analyzer, on-line signal recognition, and multithreshold archiving. We are using it to conduct a meridian transit search of the northern sky at the Harvard-Smithsonian 26-m antenna, with a second identical system scheduled to begin observations in Argentina this month. Successive 400 kHz spectra, at 0.05 Hz resolution, are searched for features characteristic of an intentional narrowband beacon transmission. These spectra are centered on guessable frequencies (such as λ21 cm), referenced successively to the local standard of rest, the galactic barycenter, and the cosmic blackbody rest frame. This search has rejected interference admirably, but is greatly limited both in total frequency coverage and sensitivity to signals other than carriers. We summarize five years of high resolution SETI at Harvard, in the context of answering the questions "How useful is narrowband SETI, how serious are its limitations, what can be done to circumvent them, and in what direction should SETI evolve?" Increasingly powerful signal processing hardware, combined with ever-higher memory densities, are particularly relevant, permitting the construction of compact and affordable gigachannel spectrum analyzers covering hundreds of megahertz of instantaneous bandwidth.

  5. Intelligent Security IT System for Detecting Intruders Based on Received Signal Strength Indicators

    Directory of Open Access Journals (Sweden)

    Yunsick Sung

    2016-10-01

    Full Text Available Given that entropy-based IT technology has been applied in homes, office buildings and elsewhere for IT security systems, diverse kinds of intelligent services are currently provided. In particular, IT security systems have become more robust and varied. However, access control systems still depend on tags held by building entrants. Since tags can be obtained by intruders, an approach to counter the disadvantages of tags is required. For example, it is possible to track the movement of tags in intelligent buildings in order to detect intruders. Therefore, each tag owner can be judged by analyzing the movements of their tags. This paper proposes a security approach based on the received signal strength indicators (RSSIs of beacon-based tags to detect intruders. The normal RSSI patterns of moving entrants are obtained and analyzed. Intruders can be detected when abnormal RSSIs are measured in comparison to normal RSSI patterns. In the experiments, one normal and one abnormal scenario are defined for collecting the RSSIs of a Bluetooth-based beacon in order to validate the proposed method. When the RSSIs of both scenarios are compared to pre-collected RSSIs, the RSSIs of the abnormal scenario are about 61% more different compared to the RSSIs of the normal scenario. Therefore, intruders in buildings can be detected by considering RSSI differences.

  6. On calibrating the sensor errors of a PDR-based indoor localization system.

    Science.gov (United States)

    Lan, Kun-Chan; Shih, Wen-Yuah

    2013-04-10

    Many studies utilize the signal strength of short-range radio systems (such as WiFi, ultrasound and infrared) to build a radio map for indoor localization, by deploying a large number of beacon nodes within a building. The drawback of such an infrastructure-based approach is that the deployment and calibration of the system are costly and labor-intensive. Some prior studies proposed the use of Pedestrian Dead Reckoning (PDR) for indoor localization, which does not require the deployment of beacon nodes. In a PDR system, a small number of sensors are put on the pedestrian. These sensors (such as a G-sensor and gyroscope) are used to estimate the distance and direction that a user travels. The effectiveness of a PDR system lies in its success in accurately estimating the user's moving distance and direction. In this work, we propose a novel waist-mounted based PDR that can measure the user's step lengths with a high accuracy. We utilize vertical acceleration of the body to calculate the user's change in height during walking. Based on the Pythagorean Theorem, we can then estimate each step length using this data. Furthermore, we design a map matching algorithm to calibrate the direction errors from the gyro using building floor plans. The results of our experiment show that we can achieve about 98.26% accuracy in estimating the user's walking distance, with an overall location error of about 0.48 m.

  7. Pathways of cellular internalisation of liposomes delivered siRNA and effects on siRNA engagement with target mRNA and silencing in cancer cells.

    Science.gov (United States)

    Alshehri, Abdullah; Grabowska, Anna; Stolnik, Snow

    2018-02-28

    Design of an efficient delivery system is a generally recognised bottleneck in translation of siRNA technology into clinic. Despite research efforts, cellular processes that determine efficiency of siRNA silencing achieved by different delivery formulations remain unclear. Here, we investigated the mechanism(s) of cellular internalisation of a model siRNA-loaded liposome system in a correlation to the engagement of delivered siRNA with its target and consequent silencing by adopting siRNA molecular beacon technology. Probing of cellular internalisation pathways by a panel of pharmacological inhibitors indicated that clathrin-mediated (dynamin-dependent) endocytosis, macropinocytosis (dynamine independent), and cell membrane cholesterol dependent process(es) (clathrin and caveolea-independent) all play a role in the siRNA-liposomes internalization. The inhibition of either of these entry routes was, in general, mirrored by a reduction in the level of siRNA engagement with its target mRNA, as well as in a reduction of the target gene silencing. A dramatic increase in siRNA engagement with its target RNA was observed on disruption of endosomal membrane (by chloroquine), accompanied with an increased silencing. The work thus illustrates that employing molecular beacon siRNA technology one can start to assess the target RNA engagement - a stage between initial cellular internalization and final gene silencing of siRNA delivery systems.

  8. Die mens geaffekteer deur kanker: Voorlopige bakens geidentifiseer met die oog op die prediking na aanleiding van Jesus se afskeidsgesprek in die Johannes-evangelie

    Directory of Open Access Journals (Sweden)

    Darius Botha

    2016-10-01

    Full Text Available The life of a person affected by cancer becomes a confusion of emotions, experiences and tumultuous events. This takes place with immediate effect and can be summarised in one word ‘havoc’. The question that comes to mind in this article is: In which way can preaching guide the person affected by cancer, to equip this person to find both sense and meaning in this life. The farewell speech of Jesus in the Gospel of John will serve as departure for the stipulation of the beacons for the preaching in the guidance of the person affected with cancer. The aim of this article is firstly to show that the person affected by cancer has specific needs concerning preaching, secondly that the farewell speech of Jesus in the Gospel of John assists the preaching with certain beacons and thirdly to motivate that the metaphor of a ‘second’ is the most suitable for this kind of preaching. The purpose with this article is that the researcher wants to emphasise that the person affected by cancer can still experience sense and meaning in this life.

  9. Bergy Bit and Melt Water Trajectories in Godthåbsfjord (SW Greenland Observed by the Expendable Ice Tracker

    Directory of Open Access Journals (Sweden)

    Daniel F. Carlson

    2017-08-01

    Full Text Available Icebergs and bergy bits makes up a significant component of the total freshwater flux from the Greenland Ice Sheet to the ocean. Observations of iceberg trajectories are biased toward larger icebergs and, as a result, the drift characteristics of smaller icebergs and bergy bits are poorly understood. In an attempt to fill this critical knowledge gap, we developed the open-source EXpendable Ice TrackEr (EXITE. EXITE is a low-cost, satellite-tracked GPS beacon capable of high-resolution temporal measurements over extended deployment periods (30 days or more. Furthermore, EXITE can transform to a surface drifter when its host iceberg capsizes or fragments. Here we describe basic construction of an EXITE beacon and present results from a deployment in Godthåbsfjord (SW Greenland in August 2016. Overall, EXITE trajectories show out-fjord surface transport, in agreement with a simple estuarine circulation paradigm. However, eddies and abrupt wind-driven reversals reveal complex surface transport pathways at time scales of hours to days.

  10. Development of a Zoo Walk Navigation System using the Positional Measurement Technology and the Wireless Communication Technology

    Directory of Open Access Journals (Sweden)

    Tomoyuki Ishida

    2016-11-01

    Full Text Available In this article, we propose and evaluate a Zoo Walk Navigation System consistings of the Animal Contents Registering and Editing Web Management System and the Animal Contents Browsing and Acquiring Smartphone Application. The Animal Contents Registering and Editing Web Management System for zoo staff enables to register/edit various animal contents. Thereby, this web management system provides real-time and flesh zoo information to the Animal Contents Browsing and Acquiring Smartphone Application. On the other hand, the Animal Contents Browsing and Acquiring Smartphone Application for zoo visitors enables to browse various animal contents which zoo staff registered through the Animal Contents Registering and Editing Web Management System. The Animal Contents Browsing and Acquiring Smartphone Application has the animal guide browsing function, the animal quiz function, the beacon notification browsing function, the zoo map navigating function, and the AR camera function. Zoo visitors can enjoy a zoo park using this smartphone application. This system is the new type navigation system which zoo staff can renew contents to avoid contents obsolescence. And, this system always provides new information to zoo visitors in real time by the beacon notification function.

  11. Electromagnetic system for detection and localization of the miners caught by accident in mine

    Science.gov (United States)

    Pronenko, Vira; Dudkin, Fedir

    2016-04-01

    It is well known that the profession of a miner is one of the most dangerous in the world. Among the main causes of the people death in the underground coal mining enterprises is their untimely alerting of the accident, as well as the lack of information for the rescuers about the actual location of the miners after the accident. As world practice shows, the electromagnetic (EM) systems for the search and detection of people across a massive layer of rock are the most effective. Such systems are under development almost half a century in many countries dealing with mine industry. However, substantial progress related to the localization of personnel at a distance at least of 20-30 meters through the rock is not reached. In an emergency situation (failure or destruction of underground infrastructure), personnel search behind and beneath of obstruction should be provided urgently. But none of the standard technologies (RFID, DECT, WiFi, emitting cable), which use the stationary technical devices in mines, do not provide notification of people caught by accident location. The only technology that provides guaranteed delivery of messages about the accident to the mine personnel, regardless of their location and under any destruction in the mine, is low-frequency radio technology able to operate through the thickness of rocks. From the general theoretical considerations, it is clear that the miners localization system requires solving the inverse problem of the magnetic field source coordinates determining using the data of 3-component magnetic field measurements. A fundamentally new approach, based on the measurement of the magnetic field of the miner's responder beacon by two fixed and spaced three-component magnetic field receivers and solution of the inverse problem using the results of the magnetic field measurement, was proposed. As a result, the concept of the equipment for miners beacon search and localization implementation (MILES - miner's location emergency

  12. FRET-Aptamer Assays for Bone Marker Assessment, C-Telopeptide, Creatinine, and Vitamin D

    Science.gov (United States)

    Bruno, John G.

    2013-01-01

    Astronauts lose 1.0 to 1.5% of their bone mass per month on long-duration spaceflights. NASA wishes to monitor the bone loss onboard spacecraft to develop nutritional and exercise countermeasures, and make adjustments during long space missions. On Earth, the same technology could be used to monitor osteoporosis and its therapy. Aptamers bind to targets against which they are developed, much like antibodies. However, aptamers do not require animal hosts or cell culture and are therefore easier, faster, and less expensive to produce. In addition, aptamers sometimes exhibit greater affinity and specificity vs. comparable antibodies. In this work, fluorescent dyes and quenchers were added to the aptamers to enable pushbutton, one-step, bind-and-detect fluorescence resonance energy transfer (FRET) assays or tests that can be freeze-dried, rehydrated with body fluids, and used to quantitate bone loss of vitamin D levels with a handheld fluorometer in the spacecraft environment. This work generated specific, rapid, one-step FRET assays for the bone loss marker C-telopeptide (CTx) when extracted from urine, creatinine from urine, and vitamin D congeners in diluted serum. The assays were quantified in nanograms/mL using a handheld fluorometer connected to a laptop computer to convert the raw fluorescence values into concentrations of each analyte according to linear standard curves. DNA aptamers were selected and amplified for several rounds against a 26- amino acid form of CTx, creatinine, and vitamin D. The commonalities between loop structures were studied, and several common loop structures were converted into aptamer beacons with a fluorophore and quencher on each end. In theory, when the aptamer beacon binds its cognate target (CTx bone peptide, creatinine, or vitamin D), it is forced open and no longer quenched, so it gives off fluorescent light (when excited) in proportion to the amount of target present in a sample. This proportional increase in fluorescence is

  13. Medium Access Control in Energy Harvesting - Wireless Sensor Networks

    DEFF Research Database (Denmark)

    Fafoutis, Xenofon

    Focusing on Wireless Sensor Networks (WSN) that are powered by energy harvesting, this dissertation focuses on energy-efficient communication links between senders and receivers that are alternating between active and sleeping states of operation. In particular, the focus lies on Medium Access......, collision avoidance, provision of Quality of Service (QoS) and security. Focusing on the particular requirements of an energy harvesting application, the dissertation continues with the presentation of a MAC protocol, named ODMAC, which extends the receiver-initiated paradigm with several energy......-efficient features that aim to adapt the consumed energy to match the harvested energy, distribute the load with respect to the harvested energy, decrease the overhead of the communication, address the requirements for collision avoidance, prioritize urgent traffic and secure the system against beacon replay attacks...

  14. Pargament's Theory of Religious Coping: Implications for Spiritually Sensitive Social Work Practice.

    Science.gov (United States)

    Xu, Jianbin

    2016-07-01

    This article proposes that Pargament's theory of religious coping can be a theoretical beacon to spiritually sensitive social work practice. It begins with a discussion of the raison d'être of spiritually sensitive social work, which is examined as being able to cast a holistic and positive glow on social work. Then it provides an overview and a critique of Pargament's theory, emphasising that the theory offers a fuller and more impartial picture of religious coping. In addition, it explores the implications of Pargament's theory for spiritually sensitive social work practice with religious clients in terms of engagement, assessment and intervention. This article concludes by discussing how social work practitioners can avoid the pitfalls and limitations of Pargament's theory.

  15. SETI: another way to find habitable worlds

    Science.gov (United States)

    Tarter, Jill

    2003-10-01

    There are currently eleven active SETI (search for extraterrestrial intelligence) programs on telescopes around the world. They operate at microwave and optical frequencies, as either sky surveys or targeted searches in the direction of (primarily) solar-type stars. Each is attempting to detect an electromagnetic biosignature of a technological civilization. Although detectable technologies will be far older than our own, these searches are constrained by our current understanding of physics and the terrestrial technologies available in the 21st century. Moore's Law suggests that most of these detection technologies will improve their capacity exponentially. Thus if any of the current strategies is valid, a thorough exploration of the Milky Way Galaxy should be possible within decades (not centuries), and success, or a significant null-result is predictable. Giving up, incorporating new search strategies based on emergent technologies, or adding beacon transmission to the listening strategies are all future possibilities.

  16. Research on Secure Localization Model Based on Trust Valuation in Wireless Sensor Networks

    Directory of Open Access Journals (Sweden)

    Peng Li

    2017-01-01

    Full Text Available Secure localization has become very important in wireless sensor networks. However, the conventional secure localization algorithms used in wireless sensor networks cannot deal with internal attacks and cannot identify malicious nodes. In this paper, a localization based on trust valuation, which can overcome a various attack types, such as spoofing attacks and Sybil attacks, is presented. The trust valuation is obtained via selection of the property set, which includes estimated distance, localization performance, position information of beacon nodes, and transmission time, and discussion of the threshold in the property set. In addition, the robustness of the proposed model is verified by analysis of attack intensity, localization error, and trust relationship for three typical scenes. The experimental results have shown that the proposed model is superior to the traditional secure localization models in terms of malicious nodes identification and performance improvement.

  17. The application of algorithm in taxi security system

    Science.gov (United States)

    Luo, Chengyu

    2017-08-01

    With the booming of the society and economy today, Taxis and private cars have gradually become one of the most popular tools in transportation for their low price and convenience. However, because of the breakdown in the security system, a few accidents occurred due to the illegal taxi. The unreliable security management has attributed to the lack of trust in taxi companies and relevant regulatory authorities, which considered to be the reason why people are worried about it. Accordingly, we put forward a design for a taxi security system, making use of modern technology such as NFC, iBeacon, GPS combined with algorithms, automatically recognize the taxi we take, and reflecting basic information of taxi and driver on our mobile phone.

  18. The development of wireless radiation dose monitoring using smart phone

    International Nuclear Information System (INIS)

    Lee, Jin Woo; Jeong, Gyo Seong; Lee, Yun Jong; Kim, Chong Yeal; Lim, Chai Wan

    2016-01-01

    Radiation workers at a nuclear facility or radiation working area should hold personal dosimeters. some types of dosimeters have functions to generate audible or visible alarms to radiation workers. However, such devices used in radiation fields these days have no functions to communicate with other equipment or the responsible personnel. our project aims at the development of a remote wireless radiation dose monitoring system that can be utilized to monitor the radiation dose for radiation workers and to notify the radiation protection manager of the dose information in real time. We use a commercial survey meter for personal radiation measurement and a smart phone for a mobile wireless communication tool and a Beacon for position detection of radiation workers using Blue tooth communication. In this report, the developed wireless dose monitoring of cellular phone is introduced

  19. Workers’ Self-management, Recovered Companies and the Sociology of Work

    Science.gov (United States)

    Croucher, Richard

    2014-01-01

    We analyse how far Argentina’s worker-recovered companies (WRCs) have sustained themselves and their principles of equity and workers’ self-management since becoming widespread following the country’s 2001–2 economic crisis. Specialist Spanish-language sources, survey data and documents are analysed through four key sociological themes. We find that the number of WRCs has increased in Argentina, and that they represent a viable production model. Further, they have generally maintained their central principles and even flourished. This occurred despite the global economic crisis, legal and financial pressures to adopt capitalist practices and management structures, the risk of market absorption and state attempts to coopt, demobilise and depoliticise the movement. We argue that today they function as a much-needed international beacon of an alternative vision for labour and that integration of their experience has potential to revitalise the field. PMID:25419009

  20. THREE HOLY MYTHS OF ARCHITECTURAL EDUCATION IN INDIA.

    Directory of Open Access Journals (Sweden)

    Srinivasan Badrinarayanan

    2014-01-01

    Full Text Available This paper addresses the largely prevalent practice of architectural pedagogy in India. There may be few exceptions to these predominant trends and they stand as important beacons of hope. The predominant pedagogy suffers from many ‘myths’ or fallacies. This paper identifies three of these myths as fundamentally deadly which need to be urgently exploded in order to pave the way to reform the education. The first myth deals with the ‘content’ or ‘what’ is taught. The second has to do with ‘how’; i.e. pedagogic bias. The third has to do with the overall philosophy of knowledge or "epistemology". The myths are: Myth 1: Architectural education = Design education = Iconic form-making. Myth 2: One can pull up average competence levels across the class by concentrating on a few geniuses in the studio. Myth 3: Delivery of knowledge can be fragmented. Integration of knowledge happens ‘automatically’ inside learners.