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Sample records for bass dicentrarchus labrax

  1. Phenanthrene and nitrite effects on juvenile sea bass, Dicentrarchus labrax, using hepatic biotransformation enzymes, biliary fluorescence, and micronuclei as biomarkers

    Digital Repository Service at National Institute of Oceanography (India)

    Reis-Henriques, M.A.; Ferreira, M.; Coimbra, A.M.; DeSilva, C.; Shailaja, M.S.

    las branquias y mediante la ingestión de sedimentos o alimentos contaminados. El fenantreno (PHE), un PAH con tres anillos, considerado uno de los PAHs prioritarios para la Agencia de Protección Ambiental de los Estados Unidos, se Phenanthrene... and nitrite effects on juvenile sea bass, Dicentrarchus labrax, using hepatic biotransformation enzymes, biliary fluorescence, and micronuclei as biomarkers Efectos del fenantreno y el nitrito en juveniles del róbalo, Dicentrarchus labrax, mediante el uso de...

  2. Seasonal variations of the humoral immune parameters of European sea bass (Dicentrarchus labrax L.)

    OpenAIRE

    Valero, Y. (Yulema)

    2014-01-01

    Seasonal cycles, mainly due to great variations in the light duration and temperature, are important and modulate several aspects of the animal behavior. In the case of poikilotherms animals such as fish this is very relevant. Thus, temperature changes fish immunity and affects disease resistance. We evaluate in this work the season variations of the European sea bass (Dicentrarchus labrax) humoral innate parameters focusing on winter months, at which the culture of this specie is more diffic...

  3. Biometry traits and geometric morphometrics in sea bass (Dicentrarchus labrax from different farming systems

    Directory of Open Access Journals (Sweden)

    Emilio Tibaldi

    2010-01-01

    Full Text Available The effect of the farming system on biometry traits and dressing out yield were inves- tigated in market-size European sea bass (Dicentrarchus labrax cultured extensively or intensively in sea cages or land-based basins. Fish external appearences and shapes were studies with geometric morphometrics in order to assess the potential of combined methodologies in the assessment of finfish quality. Both standard biometry and geometric morphometrics were able to discriminate between sea bass farmed extensively from those cultured under intensive conditions. Geometric morphometrics has been shown to be a valuable tool for describing changes in shape features and could result a useful technique to be associated to biometry traits in the context of fish quality assessment.

  4. Seasonal variations of the humoral immune parameters of European sea bass (Dicentrarchus labrax L.).

    Science.gov (United States)

    Valero, Yulema; García-Alcázar, Alicia; Esteban, M Ángeles; Cuesta, Alberto; Chaves-Pozo, Elena

    2014-08-01

    Seasonal cycles, mainly due to great variations in the light duration and temperature, are important and modulate several aspects of the animal behavior. In the case of poikilotherms animals such as fish this is very relevant. Thus, temperature changes fish immunity and affects disease resistance. We evaluate in this work the season variations of the European sea bass (Dicentrarchus labrax) humoral innate parameters focusing on winter months, at which the culture of this specie is more difficult. Our results showed that not all the innate immune parameters are depressed by low temperatures. Moreover, some of them are more dependent than others to the season and both temperature and photoperiod are operating together. PMID:24852342

  5. Concurrent environmental stressors and jellyfish stings impair caged European sea bass (Dicentrarchus labrax) physiological performances.

    Science.gov (United States)

    Bosch-Belmar, Mar; Giomi, Folco; Rinaldi, Alessandro; Mandich, Alberta; Fuentes, Verónica; Mirto, Simone; Sarà, Gianluca; Piraino, Stefano

    2016-01-01

    The increasing frequency of jellyfish outbreaks in coastal areas has led to multiple ecological and socio-economic issues, including mass mortalities of farmed fish. We investigated the sensitivity of the European sea bass (Dicentrarchus labrax), a widely cultured fish in the Mediterranean Sea, to the combined stressors of temperature, hypoxia and stings from the jellyfish Pelagia noctiluca, through measurement of oxygen consumption rates (MO2), critical oxygen levels (PO2crit), and histological analysis of tissue damage. Higher levels of MO2, PO2crit and gill damage in treated fish demonstrated that the synergy of environmental and biotic stressors dramatically impair farmed fish metabolic performances and increase their health vulnerability. As a corollary, in the current scenario of ocean warming, these findings suggest that the combined effects of recurrent hypoxic events and jellyfish blooms in coastal areas might also threaten wild fish populations. PMID:27301314

  6. Element content in cultured and wild sea bass (Dicentrarchus labrax from the Adriatic Sea

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    Žvab Rožič P.

    2013-04-01

    Full Text Available The aim of this study was to determine the levels of potentially toxic elements in cultured and wild fish tissues and to assess their risk for human health. For this purpose, sea bass specimens (Dicentrarchus labrax were sampled in selected fish farm and three other locations along the eastern Adriatic coast. Ranges of element concentrations in sea bass muscles were 1.60-4.46 ppm for As, 0.001-0.079 ppm for Cd, 0.14-49.10 ppm for Cr, 1.38-4.85 ppm for Cu, 0.11-1.31 ppm for Hg, 0.01-0.65 ppm for Pb and 21.9-136.0 ppm for Zn. Mean Cd, Cr, Cu, Hg, Pb, and Zn concentrations in commercially interesting cultured fish samples were below the permissible levels, while mean As values slightly exceed those limits. In wild fishes mean Cd, Cu, Pb and Zn concentrations were below the recommended limits, for As, Cr and Hg the mean values were higher. The smallest cultured sea bass samples showed As, Cr, Pb, and Zn concentrations exceeding the recommended limits but values decreased with fish size. Therefore, the metal concentrations in commercial fishes showed no threat for human consumption.

  7. Effects of herbal supplements on growth performance of sea bass (Dicentrarchus labrax: Change in body composition and some blood parameters

    Directory of Open Access Journals (Sweden)

    SEVDAN YILMAZ

    2012-01-01

    Full Text Available This study was conducted to investigate the effects of dietary thyme (Thymus vulgaris, rosemary (Rosmarinus officinalis and fenugreek (Trigonella foenum graecum as feed additives on growth performance, proximate composition and ammonia excretion of European sea bass Dicentrarchus labrax. Four isonitrogenous (48% crude protein and isocaloric (21 kj/g diets were formulated to contain 0% (control or 1% of thyme, rosemary or fenugreek. The thyme supplementation significantly increased protein efficiency ratio, fillet protein levels, protein and energy retentions (P0.05. The results indicate that dietary thyme improved the protein and energy retentions of sea bass.

  8. Betanodavirus ability to infect juvenile European sea bass, Dicentrarchus labrax, at different water salinity.

    Science.gov (United States)

    Pascoli, F; Serra, M; Toson, M; Pretto, T; Toffan, A

    2016-09-01

    Viral encephalopathy and retinopathy (VER) is one of the most devastating and economically relevant diseases for marine aquaculture. The presence of betanodavirus in freshwater fish is recorded, but very little is known about VER outbreaks in marine species reared in freshwater. Our study investigated the ability of betanodavirus to cause disease in European sea bass, Dicentrarchus labrax, reared at different salinity levels. Fish were challenged with RGNNV or mock infected by bath at different salinity levels (freshwater, 25‰ and 33‰). Fish were checked twice a day and the dead ones were examined by standard virological techniques, by rRT-PCR and by histochemical and immunohistochemical analyses. All the infected groups showed a significant higher mortality rate than the one of the mock-infected group. VERv presence was confirmed by rRT-PCR. Histochemical and immunohistochemical analyses highlighted the typical lesions associated with VER. Our results highlight that salinity does not affect the ability of betanodavirus to induce clinical signs and mortality in European sea bass infected under experimental conditions. These results underline the great adaptation potential of VERv, which in combination with its already known high environmental resistance and broad host range, may explain the diffusion of this disease and the threat posed to aquaculture worldwide. PMID:26763095

  9. Effects of carbofuran on the sea bass (Dicentrarchus labrax L.): study of biomarkers and behaviour alterations.

    Science.gov (United States)

    Hernández-Moreno, David; Pérez-López, Marcos; Soler, Francisco; Gravato, Carlos; Guilhermino, Lúcia

    2011-10-01

    The objective of this study was to investigate the acute effects of the pesticide carbofuran on the sea bass (Dicentrarchus labrax) using parameters at different levels of biological organisation (swimming behaviour and several biomarkers) and possible relationships between alterations found in different effect criteria. In a bioassay, sea bass juveniles were individually exposed to different doses of carbofuran (31, 63, 125 and 250 μg/L) for 96 h. At the end of the bioassay, the swimming performance and 11 biomarkers were determined. Biomarkers were: hepatosomatic index (HSI), lipid peroxidation (LPO), reduced glutathione and the activities of the enzymes ethoxyresorufin O-deethylase (EROD), glutathione S-transferases, superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase, brain acetylcholinesterase (AChE) and muscle cholinesterases (ChE). After 96 h of exposure, carbofuran induced a decrease of the swimming velocity and inhibition of EROD activity at all concentrations tested, and inhibition of muscle ChE and brain AChE activities at 250 μg/L. No relevant alterations in any of the other tested parameters were found. These results show that carbofuran induced adverse effects on fish by interfering with neurofunction, capability of detoxication and swimming velocity. In addition, positive and significant correlations between the swimming velocity and (i) brain AChE activity, (ii) muscle ChE activity and (iii) EROD activity suggest that the inhibition of these enzymes may somehow be related to the behavioural changes observed. Since these functions are determinant for the survival and performance of the fish in the wild, the findings of the present study suggest that adverse effects may occur in populations exposed to carbofuran if a sufficient number of animals is affected.

  10. Behavioural stress responses predict environmental perception in European sea bass (Dicentrarchus labrax.

    Directory of Open Access Journals (Sweden)

    Sandie Millot

    Full Text Available Individual variation in the response to environmental challenges depends partly on innate reaction norms, partly on experience-based cognitive/emotional evaluations that individuals make of the situation. The goal of this study was to investigate whether pre-existing differences in behaviour predict the outcome of such assessment of environmental cues, using a conditioned place preference/avoidance (CPP/CPA paradigm. A comparative vertebrate model (European sea bass, Dicentrarchus labrax was used, and ninety juvenile individuals were initially screened for behavioural reactivity using a net restraining test. Thereafter each individual was tested in a choice tank using net chasing as aversive stimulus or exposure to familiar conspecifics as appetitive stimulus in the preferred or non preferred side respectively (called hereafter stimulation side. Locomotor behaviour (i.e. time spent, distance travelled and swimming speed in each tank side of each individual was recorded and analysed with video software. The results showed that fish which were previously exposed to appetitive stimulus increased significantly the time spent on the stimulation side, while aversive stimulus led to a strong decrease in time spent on the stimulation side. Moreover, this study showed clearly that proactive fish were characterised by a stronger preference for the social stimulus and when placed in a putative aversive environment showed a lower physiological stress responses than reactive fish. In conclusion, this study showed for the first time in sea bass, that the CPP/CPA paradigm can be used to assess the valence (positive vs. negative that fish attribute to different stimuli and that individual behavioural traits is predictive of how stimuli are perceived and thus of the magnitude of preference or avoidance behaviour.

  11. Expression and localization of Aquaporin 1a in the sea-bass (Dicentrarchus labrax during ontogeny

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    Ivone eGiffard-Mena

    2011-07-01

    Full Text Available The successful establishment of a species in a given habitat depends on the ability of each of its developing stages to adapt to the environment. In order to understand this process we have studied the adaptation of a euryhaline fish, the sea-bass Dicentrarchus labrax, to various salinities during its ontogeny. The expression and localization of Aquaporin 1a (AQP1a mRNA and protein were determined in different osmoregulatory tissues. In larvae, the sites of AQP1a expression are variable and they shift according to age, implying functional changes. In juveniles after metamorphosis (D32-48 post hatch, 15 - 25 mm and in pre-adults, an increase in AQP1a transcript abundance was noted in the digestive tract, and the AQP1a location was observed in the intestine. In juveniles (D87-100 post hatch, 38 - 48 mm, the transcript levels of AQP1a in the digestive tract and in the kidney were higher in sea water than at lower salinity. These observations, in agreement with existing models, suggest that in sea water-acclimated fish, the imbibed water is absorbed via AQP1a through the digestive tract, particularly the intestine and the rectum. In addition, AQP1a may play a role in water reabsorption in the kidney. These mechanisms compensate dehydratation in sea water, and they contribute to the adaptation of juveniles to salinity changes during sea-lagoon migrations. These results contribute to the interpretation of the adaptation of populations to habitats where salinity varies.

  12. Lack of evidence for a role of olfaction on first maturation in farmed sea bass Dicentrarchus labrax.

    Science.gov (United States)

    Saraiva, João L; Martins, Rute S; Hubbard, Peter C; Canário, Adelino V M

    2015-09-15

    Chemical communication is widespread in the animal kingdom and olfaction constitutes a powerful channel for social and environmental cues. In fish, olfactory stimuli are known to influence physiological processes, including reproduction. Here we investigate the effects of olfaction on puberty in European sea bass Dicentrarchus labrax males. Intact sea bass coming to first maturity (puberty) are able to smell conspecific odours. However, induced anosmia during most of the spermatogenesis period had no effect on the sex ratio, gonad maturation state or gonado-somatic index at the time of reproduction. Furthermore anosmia decreased mRNA expression of brain KISS2 and pituitary LHb and FSHb, but not brain GnRH1 and GnRH3. Thus, although anosmia seems to modify gene expression of key reproduction related genetic factors, it seems to be insufficient to stop or delay growth or gonadal development and maturation.

  13. Does Virulence Assessment of Vibrio anguillarum Using Sea Bass (Dicentrarchus labrax) Larvae Correspond with Genotypic and Phenotypic Characterization?

    DEFF Research Database (Denmark)

    Frans, Ingeborg; Dierckens, Kristof; Crauwels, Sam;

    2013-01-01

    Background: Vibriosis is one of the most ubiquitous fish diseases caused by bacteria belonging to the genus Vibrio such as Vibrio (Listonella) anguillarum. Despite a lot of research efforts, the virulence factors and mechanism of V. anguillarum are still insufficiently known, in part because of the......'s Phenotype MicroArray (TM) technology and some virulence factor assays. Conclusions/Significance: Virulence testing revealed ten virulent and five avirulent strains. While some relation could be established between serotype, genotype and phenotype, no relation was found between virulence and genotypic or...... lack of standardized virulence assays. Methodology/Principal Findings: We investigated and compared the virulence of 15 V. anguillarum strains obtained from different hosts or non-host niches using a standardized gnotobiotic bioassay with European sea bass (Dicentrarchus labrax L.) larvae as model...

  14. Influence of dietary vitamin C dosage on turbot (Scophthalmus maximus) and European sea bass (Dicentrarchus labrax) nursery stages

    OpenAIRE

    Merchie, G.; Lavens, P.; Storch, V.; Übel, U.; Nelis, H.; De leenheer, A P; Sorgeloos, P.

    1996-01-01

    Stable forms of vitamin C were verified as dietary sources of ascorbic acid (AA) for the nursery stages of European sea bass and turbot. In a first experiment, various concentrations of ascorbyl palmitate (AP) and one level of AA 2-polyphosphate (ApP) were evaluated in a semi purified diet for European sea bass, Dicentrarchus labrax. A significantly lower AA concentration was detected in the fry fed the AP-supplemented diets compared to the ApP-fed group, providing evidence that AP is an infe...

  15. Intraspecific individual variation of temperature tolerance associated with oxygen demand in the European sea bass (Dicentrarchus labrax).

    Science.gov (United States)

    Ozolina, Karlina; Shiels, Holly A; Ollivier, Hélène; Claireaux, Guy

    2016-01-01

    The European sea bass (Dicentrarchus labrax) is an economically important fish native to the Mediterranean and Northern Atlantic. Its complex life cycle involves many migrations through temperature gradients that affect the energetic demands of swimming. Previous studies have shown large intraspecific variation in swimming performance and temperature tolerance, which could include deleterious and advantageous traits under the evolutionary pressure of climate change. However, little is known of the underlying determinants of this individual variation. We investigated individual variation in temperature tolerance in 30 sea bass by exposing them to a warm temperature challenge test. The eight most temperature-tolerant and eight most temperature-sensitive fish were then studied further to determine maximal swimming speed (U CAT), aerobic scope and post-exercise oxygen consumption. Finally, ventricular contractility in each group was determined using isometric muscle preparations. The temperature-tolerant fish showed lower resting oxygen consumption rates, possessed larger hearts and initially recovered from exhaustive exercise faster than the temperature-sensitive fish. Thus, whole-animal temperature tolerance was associated with important performance traits. However, the temperature-tolerant fish also demonstrated poorer maximal swimming capacity (i.e. lower U CAT) than their temperature-sensitive counterparts, which may indicate a trade-off between temperature tolerance and swimming performance. Interestingly, the larger relative ventricular mass of the temperature-tolerant fish did not equate to greater ventricular contractility, suggesting that larger stroke volumes, rather than greater contractile strength, may be associated with thermal tolerance in this species. PMID:27382468

  16. Effects of dietary yeast Saccaromyces cerevisiae on the antioxidant system in the liver of juvenile sea bass Dicentrarchus labrax.

    Science.gov (United States)

    Santacroce, Maria Pia; Merra, Elisabetta; Centoducati, Gerardo; Zacchino, Valentina; Casalino, Elisabetta

    2012-10-01

    The main goal of this work was to determine the effect of dietary live yeast Saccharomyces cerevisiae on the oxidative status of sea bass Dicentrarchus labrax juveniles. Fishes were fed on three diets: the GM group were fed a diet containing lyophilized yeast grown on grape must, the CS group were fed a diet containing lyophilized yeast grown on cornstarch, and the control group were fed a diet without yeast. The activity of the main antioxidative enzymes, superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase, glutathione S-transferase (GST), and glutathione (GSH) content, as well as lipid peroxidation, was measured in the liver of sea bass juveniles 90 days after hatching. Supplementation of the diet with S. cerevisiae significantly reduced the SOD and CAT activity, increased the GST activity, decreased the GSH content, and had no effect on lipid peroxidation. The results support the already reported radical-scavenging properties of yeast and usefulness of its employment as antiperoxidative agent in fish. PMID:22484599

  17. Autochthonous Bacterial Isolates Successfully Stimulate In vitro Peripheral Blood Leukocytes of the European Sea Bass (Dicentrarchus labrax).

    Science.gov (United States)

    Mladineo, Ivona; Bušelić, Ivana; Hrabar, Jerko; Radonić, Ivana; Vrbatović, Anamarija; Jozić, Slaven; Trumbić, Željka

    2016-01-01

    Commercially available probiotics are routinely administered as feed supplements in aquaculture important species. Among them, the European sea bass (Dicentrarchus labrax) is the most widely reared fish in the Mediterranean, whose rearing systems are highly variable between countries, affecting at some level the sustainability of production. After random isolation of autochthonous gut bacteria of the sea bass, their identification and pathogenicity testing, we have selected three potentially probiotic isolates; Pseudoalteromonas sp., Alteromonas sp., and Enterovibrio coralii. Selected isolates were tested and their immunostimulative efficiency was compared with a commercially available Lactobacillus casei isolate, inferring inflammatory, apoptotic and anti-pathogen response of sea bass' peripheral blood leukocytes. Phagocytic activity, respiratory burst, and expression of lysozyme, Mx protein, caspase 3, TNF-α, IL-10 genes was measured 1, 3, 5, and 12 h post-stimulation by four bacterial isolates to evaluate early kinetics of the responses. Best immunostimulative properties were observed in Pseudoalteromonas-stimulated leukocytes, followed by Alteromonas sp. and L. casei, while Enterovibrio coralii failed to induce significant stimulation. Based on such in vitro assay intestinal autochthonous bacterial isolates showed to have better immunostimulative effect in sea bass compared to aquaculture-widely used L. casei, and further steps need to engage tank and field feeding trials to evaluate long-term prophylactic suitability of the chosen isolates. A panel of biomarkers that represent pro-/anti-inflammatory, pro-/anti-apoptotic, and anti-bacteria/viral responses of the fish should be taken into consideration when evaluating the usefulness of the potential probiotic in aquaculture. PMID:27551281

  18. A decadal trend of juvenile European sea bass (Dicentrarchus labrax, L. responses to climate patterns in the Mondego estuary, Portugal

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    Eduardo Granja Bento

    2015-11-01

    Full Text Available Estuarine systems support the life cycle stages of commercially important marine fish and are influenced by large and local-scale climatic patterns. Also, extreme events triggered by climate changes may influence the functioning of nursery grounds and recruitment for several fish species. In this study, performed in the Mondego estuary, Portugal, we used an 11-year database (2003-2013 for analyzing the variability in the population of a marine juvenile migrant fish, the European sea bass Dicentrarchus labrax, regarding changes in abundance, population structure, growth rates and secondary production and annual day of peak abundance. Higher densities and production occurred at the beginning of the study, but no differences in 0-group growth could be observed. In order to detect change points in both biological and climatic data, the cumulative sum (CUSUM of the deviations from the mean for the 2003-2013 period were determined for each parameter. The relationship between large- and local-scale drivers and 0-group abundance, secondary production and day of peak abundance were evaluated using a Pearson correlation analysis of CUSUM of biological and environmental data, considering the correspondent yearly values and with a time-lag of 1 year. The North Atlantic Oscillation (NAO index, sea surface temperature (SST and their respective winter values were tested as large-scale factors, while river runoff, salinity and water temperature were considered as local climate patterns. River runoff was the significant factor explaining D. labrax 0-group abundances and the NAO and water temperature were also significant predictors considering the 1-year lag. Regarding D. labrax 0-group secondary production, salinity and water temperature were the significant predictors. The NAO with 1-year lag was also negatively correlated with the day of peak abundance. The observed variability regarding yearly trends in abundance of juvenile fish was mostly linked to local

  19. Does virulence assessment of Vibrio anguillarum using sea bass (Dicentrarchus labrax larvae correspond with genotypic and phenotypic characterization?

    Directory of Open Access Journals (Sweden)

    Ingeborg Frans

    Full Text Available BACKGROUND: Vibriosis is one of the most ubiquitous fish diseases caused by bacteria belonging to the genus Vibrio such as Vibrio (Listonella anguillarum. Despite a lot of research efforts, the virulence factors and mechanism of V. anguillarum are still insufficiently known, in part because of the lack of standardized virulence assays. METHODOLOGY/PRINCIPAL FINDINGS: We investigated and compared the virulence of 15 V. anguillarum strains obtained from different hosts or non-host niches using a standardized gnotobiotic bioassay with European sea bass (Dicentrarchus labrax L. larvae as model hosts. In addition, to assess potential relationships between virulence and genotypic and phenotypic characteristics, the strains were characterized by random amplified polymorphic DNA (RAPD and repetitive extragenic palindromic PCR (rep-PCR analyses, as well as by phenotypic analyses using Biolog's Phenotype MicroArray™ technology and some virulence factor assays. CONCLUSIONS/SIGNIFICANCE: Virulence testing revealed ten virulent and five avirulent strains. While some relation could be established between serotype, genotype and phenotype, no relation was found between virulence and genotypic or phenotypic characteristics, illustrating the complexity of V. anguillarum virulence. Moreover, the standardized gnotobiotic system used in this study has proven its strength as a model to assess and compare the virulence of different V. anguillarum strains in vivo. In this way, the bioassay contributes to the study of mechanisms underlying virulence in V. anguillarum.

  20. Carbonate precipitates and bicarbonate secretion in the intestine of sea bass, Dicentrarchus labrax.

    Science.gov (United States)

    Faggio, Caterina; Torre, Agata; Lando, Gabriele; Sabatino, Giuseppe; Trischitta, Francesca

    2011-05-01

    The aim of this paper was to study the chemical composition of the precipitates found in the intestine of Dicentrarchus labrax and the source of HCO(3)(-) secreted into the intestinal lumen. The chemical analysis was performed by employing the potentiometric double titration method and by means of an electron microscope coupled with a spectrometer and X-ray powder diffraction. The results obtained suggest the presence of very insoluble intestinal precipitates, presumably formed by a mixture of CaCO(3) and MgCO(3), with a higher quantity of the former with respect to the latter. HCO(3)(-) secretion rate was investigated with the aid of the pH stat method in isolated tissues mounted in Ussing chamber, where the transepithelial electrical parameters were also measured. When the serosal surface of the intestinal mucosa was bathed in HCO(3)(-)-Ringer bubbled with 1% CO(2) in O(2) while the serosal surface was bathed in HCO(3)(-) free Ringer solution bubbled with pure O(2), bicarbonate secretion proceeded at an almost stable rate of 0.9 ± 0.05 μeq cm(-2) h(-1) for about 3 h while I(sc) maintained a constant value of 38 ± 1.5 μA cm(-2). The carbonic anhydrase inhibitor ethoxyzolamide elicited a progressive reduction of HCO(3)(-) secretion that was about 75% of the initial value after 80 min. When serosal HCO(3)(-)-CO(2) saline was substituted with Hepes-O(2) saline base secretion progressively declined reaching a value of about 20% of the initial value. It was also strongly inhibited when Na(+) was substituted with the impermeant cation choline and when either DIDS or ouabain were added to the basolateral side. These results suggest that most of the bicarbonate secreted is of extracellular source and is probably transported across the basolateral membrane by both Na(+) independent mechanism and Na(+) dependent transporter, presumably a NaHCO(3) cotransport. PMID:21152925

  1. Effect of temperature on maximum swimming speed and cost of transport in juvenile European sea bass (Dicentrarchus labrax).

    Science.gov (United States)

    Claireaux, Guy; Couturier, Christine; Groison, Anne-Laure

    2006-09-01

    This study is an attempt to gain an integrated understanding of the interactions between temperature, locomotion activity and metabolism in the European sea bass (Dicentrarchus labrax). To our knowledge this study is among the few that have investigated the influence of the seasonal changes in water temperature on swimming performance in fish. Using a Brett-type swim-tunnel respirometer the relationship between oxygen consumption and swimming speed was determined in fish acclimatised to 7, 11, 14, 18, 22, 26 and 30 degrees C. The corresponding maximum swimming speed (U(max)), optimal swimming speed (U(opt)), active (AMR) and standard (SMR) metabolic rates as well as aerobic metabolic scope (MS) were calculated. Using simple mathematical functions, these parameters were modelled as a function of water temperature and swimming speed. Both SMR and AMR were positively related to water temperature up to 24 degrees C. Above 24 degrees C SMR and AMR levelled off and MS tended to decrease. We found a tight relationship between AMR and U(max) and observed that raising the temperature increased AMR and increased swimming ability. However, although fish swam faster at high temperature, the net cost of transport (COT(net)) at a given speed was not influence by the elevation of the water temperature. Although U(opt) doubled between 7 degrees C and 30 degrees C (from 0.3 to 0.6 m s(-1)), metabolic rate at U(opt) represented a relatively constant fraction of the animal active metabolic rate (40-45%). A proposed model integrates the effects of water temperature on the interaction between metabolism and swimming performance. In particular the controlling effect of temperature on AMR is shown to be the key factor limiting maximal swimming speed of sea bass.

  2. Fertilization strategies for Sea Bass Dicentrarchus labrax (Linnaeus, 1758): effects of pre-incubation and duration of egg receptivity in seawater

    DEFF Research Database (Denmark)

    Siddique, Mohammad A Momin; Butts, Ian; Linhart, Otomar;

    2015-01-01

    these treatments after 3 min of pre-incubation, which clearly indicates that sea bass eggs are able to be fertilized by sperm for up to 3 min after release into seawater. This study has particular importance for understanding fertilization strategies, reproductive potential, as well as reproductive ecology of sea......Studying gamete biology can provide important information about a species fertilization strategy as well as their reproductive ecology. Currently, there is a lack of knowledge about how long sea bass Dicentrarchus labrax eggs can remain viable after being activated in seawater. The objectives...... of this study were to understand the effects of pre-incubation of fresh and overripe sea bass eggs in seawater and to determine the duration of egg receptivity. Pooled eggs (fresh and overripe) from four females were pre-incubated in seawater for 0 min (control), 0.5 min, 1 min, 3 min, 10 min and 30 min...

  3. Effects of persistent organic pollutants on the thyroid function of the sea bass (Dicentrarchus labrax), an endocrine disruption?

    OpenAIRE

    Schnitzler, Joseph; Koutrakis, Emmanuil; Thomé, Jean-Pierre; Siebert, Ursula; Das, Krishna

    2007-01-01

    Organochlorinated compounds such as polychlorobiphenyls (PCB), dichloro-diphenyl-dichloroethylene (DDE) and dichloro-diphenyl-trichloroethane (DDT) have the characteristic to influence the endocrine system of many fish species and mammals and are thus qualified as endocrine disruptors. The objective of this project aims a better understanding of the effects of these compounds on the thyroid function of European seabass (Dicentrarchus labrax). Thyroid tissue and muscle of 46 seabass from t...

  4. In vitro immunotoxicological effects of heavy metals on European sea bass (Dicentrarchus labrax L.) head-kidney leucocytes.

    Science.gov (United States)

    Morcillo, Patricia; Cordero, Héctor; Meseguer, José; Esteban, M Ángeles; Cuesta, Alberto

    2015-11-01

    The knowledge about the direct effects of heavy metals on fish leucocytes is still limited. We investigate the in vitro effects of heavy metals (Cd, Hg, Pb or As) on oxidative stress, viability and innate immune parameters of head-kidney leucocytes (HKLs) from European sea bass (Dicentrarchus labrax). Production of free oxygen radicals was induced by Cd, Hg and As, mainly after 30 min of exposure. Cd and Hg promoted both apoptosis and necrosis cell death while Pb and As did only apoptosis, in all cases in a concentration-dependent manner. Moreover, expression of genes related to oxidative stress and apoptosis was significantly induced by Hg and Pb but down-regulated by As. In addition, the expression of the metallothionein A gene was up-regulated by Cd and Pb exposure though this transcript, as well as the heat shock protein 70, was down-regulated by Hg. Cd, methylmercury (MeHg) and As reduced the phagocytic ability, whereas Hg and Pb increased it. Interestingly, all the heavy metals decreased the phagocytic capacity (the number of ingested particles per cell). Leucocyte respiratory burst changed depending on the metal exposure, usually in a time- and dose-manner. Interestingly, the expression of immune-related genes was slightly affected by Cd, MeHg, As or Pb being Hg the form producing the greatest alterations, which included down-regulation of immunoglobulin M and hepcidin, as well as the up-regulation of interleukin-1 beta mRNA levels. This study provides an in vitro approach for elucidating the heavy metals toxicity, and particularly the immunotoxicity, in fish leucocytes. PMID:26363228

  5. Effects of using slurry ice during transportation on the microbiological, chemical, and sensory assessments of aquacultured sea bass (Dicentrarchus labrax) stored at 4 degrees C.

    Science.gov (United States)

    Cakli, Sukran; Kilinc, Berna; Dincer, Tolga; Tolasa, Sebnem

    2006-01-01

    Slurry ice, a biphasic system consisting of small spherical ice crystals surrounded by seawater at subzero temperature, was evaluated as a new chilled storage method for whole sea bass (Dicentrarchus labrax) a sparidae fish species of remarkable commercial interests. In this study two different group of chilling methods were used during transportation; in slurry ice packaged (Group A), and flake ice packaged (Group B). The effect of this advanced system during transportation on quality losses and the shelf life of aquacultured sea bass was evaluated. Mesophilic counts for sea bass exceeded 7 log cfu/g, which is considered the maximum level for acceptability for freshwater and marine fish after 13 days for groups A and B. On day 13 TVB-N values of groups A and B, reached the legal limits (35 mg/100 g set for TVB-N) for consumption. According to the results of sensory analyses, up to day 9 all the groups were determined as "acceptable" but on day 13 the groups A and B were no longer acceptable. The main negative aspect related to quality loss in slurry ice group corresponded to the appearance of eyes and gills. Using slurry ice during transportation did not extend the shelf life of sea bass stored at 4 degrees C. PMID:16864138

  6. Cellular and molecular immune responses of the sea bass (Dicentrarchus labrax) experimentally infected with betanodavirus

    DEFF Research Database (Denmark)

    Scapigliati, G.; Buonocore, F.; Randelli, E.;

    2010-01-01

    and acquired responses: type I IFN, Mx, IL-1, Cox-2; IL-10, TGF-β, TCRβ, CD4, CD8α, IgM, by using a quantitative PCR array system developed for sea bass. The obtained results showed a detectable increase of T cells and B cells in PBL during betanodavirus infection. Furthermore, leucocytes obtained from blood......Naïve sea bass juveniles (38.4 ± 4.5 g) were intramuscularly infected with a sublethal dose of betanodavirus isolate 378/I03, followed after 43 days by a similar boosting. This infection resulted in an overall mortality of 7.6%. At various intervals, sampling of fish tissues was performed...... after infection, despite the presence of measurable levels of natural antibody. Finally, a strong upregulation of genes coding for type I IFN, Mx, and IgM was identified after both infection and boosting. Interestingly, an upregulation of Cox-2 until boosting, and of TGF-β and IL-10 after boosting...

  7. Quantitative immunoenzymatic detection of viral encephalopathy and retinopathy virus (betanodavirus) in sea bass Dicentrarchus labrax.

    Science.gov (United States)

    Nuñez-Ortiz, N; Stocchi, V; Toffan, A; Pascoli, F; Sood, N; Buonocore, F; Picchietti, S; Papeschi, C; Taddei, A R; Thompson, K D; Scapigliati, G

    2016-07-01

    Viral encephalopathy and retinopathy disease caused by betanodavirus, genus of the family Nodaviridae, affects marine, wild and farmed species including sea bass, one of the most important farmed species in Europe. This work describes a reliable and sensitive indirect ELISA assay to detect betanodavirus in biological samples using a polyclonal antiserum (pAb 283) against the 283/I09 virus strain, the most common red-spotted grouper nervous necrosis virus (RGNNV) genotype in the Mediterranean area, and a capture-based ELISA using a monoclonal antibody (mAb 4C3) specific to a common epitope present on the capsid protein. Using adsorbed, purified VERv preparation, the detection limit of indirect ELISA was 2 μg mL(-1) (3 × 10(5) TCID50 per mL), whereas for capture-based ELISA, the sensitivity for the antigen in solution was 17 μg mL(-1) (35 × 10(5) TCID50 per mL). The capture-based ELISA was employed to detect VERv in brain homogenates of in vivo infected sea bass and resulted positive in 22 of 32 samples, some of these with a high viral load estimates (about 1.1 × 10(8)  TCID50 per mL). The ELISA system we propose may be helpful in investigations where coupling of viral content in fish tissues with the presence of circulating VERv-specific IgM is required, or for use in samples where PCR is difficult to perform. PMID:26610431

  8. Mycobiota and Aflatoxin B1 in Feed for Farmed Sea Bass (Dicentrarchus labrax

    Directory of Open Access Journals (Sweden)

    Fernando Manuel d´Almeida Bernardo

    2011-02-01

    Full Text Available The safety characteristics of feed used in fish and crustacean aquaculture systems are an essential tool to assure the productivity of those animal exploitations. Safety of feed may be affected by different hazards, including biological and chemical groups. The aim of this preliminary study was to evaluate fungi contamination and the presence of aflatoxins in 87 samples of feed for sea bass, collected in Portugal. Molds were found in 35 samples (40.2% in levels ranging from 1 to 3.3 log10 CFU∙g−1. Six genera of molds were found. Aspergillus flavus was the most frequent, found in all positive samples, with a range from 2 to 3.2 log10 CFU∙g−1. Aspergillus niger was found in 34 samples (39.1%, ranging from 1 to 2.7 log10 CFU∙g−1. Aspergillus glaucus was found in 26 samples (29.9% with levels between 1 and 2.4 log10 CFU∙g−1. Penicillium spp. and Cladosporium spp. were both found in 25 samples (28.7%. Fusarium spp. was found in 22 samples (25.3%, ranging from 1 to 2.3 log10 CFU∙g−1. All feed samples were screened for aflatoxins using a HPLC technique, with a detection limit of 1.0 μg∙kg−1. All samples were aflatoxin negative.

  9. The effect of density on sea bass (Dicentrarchus labrax) performance in a tank-based recirculating system

    OpenAIRE

    Sammouth, Sophie; Roque d'Orbcastel, Emmanuelle; Gasset, Eric; Lemarie, Gilles; Breuil, Gilles; Marino, Giovanna; Coeurdacier, Jean-luc; Fivelstad, Sveinung; Blancheton, Jean-Paul

    2009-01-01

    Sea bass (Dicentrarchus tabrax) (135 +/- 4 g) were reared under tank-based recirculating aquaculture system for a 63-day period at four densities: 10, 40, 70, 100 kg m(-3). Fish performance, stress indicators (plasma cortisol, proteonemia plus other blood parameters-Na+, K+, glucose, pH, total CO2- and water quality were monitored. At the end of the 63-day period, resistance to infection was also studied by a nodavirus challenge. A 25-day test was performed on fish from two extreme densities ...

  10. Individual variation and repeatability in aerobic and anaerobic swimming performance of European sea bass, Dicentrarchus labrax.

    Science.gov (United States)

    Marras, S; Claireaux, G; McKenzie, D J; Nelson, J A

    2010-01-01

    Studies of inter-individual variation in fish swimming performance may provide insight into how selection has influenced diversity in phenotypic traits. We investigated individual variation and short-term repeatability of individual swimming performance by wild European sea bass in a constant acceleration test (CAT). Fish were challenged with four consecutive CATs with 5 min rest between trials. We measured maximum anaerobic speed at exhaustion (U(CAT)), gait transition speed from steady aerobic to unsteady anaerobic swimming (U(gt)), routine metabolic rate (RMR), post-CAT maximum metabolic rate (MMR), aerobic scope and recovery time from the CATs. Fish achieved significantly higher speeds during the first CAT (U(CAT)=170 cm s(-1)), and had much more inter-individual variation in performance (coefficient of variation, CV=18.43%) than in the subsequent three tests (U(CAT)=134 cm s(-1); CV=7.3%), which were very repeatable among individuals. The individual variation in U(CAT) in the first trial could be accounted for almost exclusively by variation in anaerobic burst-and-coast performance beyond U(gt). The U(gt) itself varied substantially between individuals (CV=11.4%), but was significantly repeatable across all four trials. Individual RMR and MMR varied considerably, but the rank order of post-CAT MMR was highly repeatable. Recovery rate from the four CATs was highly variable and correlated positively with the first U(CAT) (longer recovery for higher speeds) but negatively with RMR and aerobic scope (shorter recovery for higher RMR and aerobic scope). This large variation in individual performance coupled with the strong correlations between some of the studied variables may reflect divergent selection favouring alternative strategies for foraging and avoiding predation.

  11. Loss of genetic variation in Greek hatchery populations of the European sea bass (Dicentrarchus labrax L. as revealed by microsatellite DNA analysis

    Directory of Open Access Journals (Sweden)

    D. LOUKOVITIS

    2015-01-01

    Full Text Available Genetic variation in four reared stocks of European sea bass Dicentrarchus labrax L., originating from Greek commercial farms, was assessed using five polymorphic microsatellite markers and was compared with that of three natural populations from Greece and France. The total number of alleles per marker ranged from 8 to 22 alleles, and hatchery samples showed the same levels of observed heterozygosity with samples from the wild but substantially smaller allelic richness and expected heterozygosity. The genetic differentiation of cultivated samples between them as well as from the wild origin fish was significant as indicated by Fst analysis. All population pairwise comparisons were statistically significant, except for the pair of the two natural Greek populations. Results of microsatellite DNA analysis herein showed a 37 % reduction of the mean allele number in the hatchery samples compared to the wild ones, suggesting random genetic drift and inbreeding events operating in the hatcheries. Knowledge of the genetic variation in D. labrax cultured populations compared with that in the wild ones is essential for setting up appropriate guidelines for proper monitoring and management of the stocks either under traditional practices or for the implementation of selective breeding programmes.

  12. The influence of different dietary energy content and feeding regimes on growth and feed utilization of European sea bass (Dicentrarchus labrax, L.

    Directory of Open Access Journals (Sweden)

    Pier Paolo Gatta

    2010-01-01

    Full Text Available The growing importance of European sea bass (Dicentrarchus labrax in aquaculture underlines the need to optimize the feeding strategy for this fish species. The aim of the present study was to assess the effect of dietary energy content and feeding regime on growth performance, feed uti- lization and feeding costs for European sea bass. Seven hundreds and forthyfourfish(averageinitial forthyfourfish(averageinitial four fish ( average initial body weight 68g were randomly allocated into twelve tanks 800 l in a closed recirculation system (water temperature: 22°C; dissolved oxygen ≥90% of saturation. Three isoproteic (47% crude protein extruded diets were formulated with different lipid levels i.e. 16% (diet D16, 24% (diet D24 and 32% (diet D32 and each diet was fed at two different feeding regimes (satiation and 80% satiation accord- ing to a bifactorial experimental design. Feed intake (FI was recorded daily. After 77 days, fish were bulk weighed and growth, SGR and FCR were calculated. Feedingregimesaffectedall theanalysed Feeding regimes affected all the analysed parameters (P<0.05, whereas diet influenced only FCR, FI, protein and lipid intake and the economic efficiency ratio (EER. Fish fed the lowest energy content diet (D16 to satiation resulted in the highest feed intake, a FCR similar to that of fish fed diets D24 and D32 and in the lowest EER.

  13. Molecular characterization and in vivo expression of hypoxia inducible factor (HIF-1α in sea bass (Dicentrarchus labrax exposed to acute and chronic hypoxi

    Directory of Open Access Journals (Sweden)

    Marco Saroglia

    2010-01-01

    Full Text Available Aquatic hypoxia is a frequent event and in fish a complex set of physiological and bi- ochemical alterations are employed to cope with this environmental stress. Many of these adjustments depend to a large extent on changes in the expression of genes that encode for physiologically relevant proteins. Genes that are induced by hypoxia appear to share a common mode of transcriptional regula- tion. This induction depends upon activation of a transcription factor, the hypoxia inducible factor-1 (HIF-1, which is a heterodimer composed of two subunits: α ‧ and β. In this study we report first on the molecular cloning and characterization of HIF-1α ‧ in sea bass (Dicentrarchus labrax. The full-length sea bass cDNA for HIF-1α was isolated and deposited in the GenBank with accession no. DQ171936. It consists of 3317 base pairs (bp carrying a single open-read- ing frame that encompasses 2265 bp of the coding region and 1052 bp of the 3’ UTR. We then utilized the real-time PCR technology to monitor dynamic changes in levels of HIF-1α ‧ tran- scripts, in response to acute and chronic hypoxic stress. The number of HIF-1α ‧ mRNA copies were signifi- cantly increased in response to both acute (1.9 mg/L, dissolved oxygen for 4 hours and chronic (4.3 mg/L, DO for 15 days hypoxia in sea bass, whereas it remained unchanged in fish exposed to hyperoxic (DO 13.5±1.2 mg/L, 155 % saturation conditions. This is the first study carried out to investigate the behaviour of HIF-1α gene transcripts during hypoxia in representative of marine hypoxia-sensitive fish species.

  14. Biological effects of diethylene glycol (DEG) and produced waters (PWs) released from offshore activities: A multi-biomarker approach with the sea bass Dicentrarchus labrax

    International Nuclear Information System (INIS)

    Diethylene glycol (DEG) is largely used during oil and gas exploitation by offshore platforms. The aim of this work was to investigate if this compound induces direct molecular/cellular effects in marine organisms, or indirectly modulate those of produced waters (PWs). Sea bass (Dicentrarchus labrax) were exposed to DEG dosed alone or in combination with PWs from an Adriatic platform. A wide array of analysed biomarkers included cytochrome P450-dependent enzymatic activity, bile metabolites, glutathione S-transferases, acetylcholinesterase, peroxisomal proliferation, antioxidant defences (catalase, glutathione reductase, glutathione peroxidases, glutathione), total oxyradical scavenging capacity, malondialdehyde and DNA integrity (single strand breaks and frequency of micronuclei). Results did not reveal marked effects of DEG, while PWs influenced the biotransformation system, the oxidative status and the onset of genotoxic damages. Co-exposures caused only limited differences of biomarker responses at some experimental conditions, overall suggesting a limited biological impact of DEG at levels normally deriving from offshore activities. - A biological risk for marine organisms can be excluded for DEG concentrations as those normally associated to produced waters discharged in the Adriatic Sea.

  15. In situ mortality experiments with juvenile sea bass (Dicentrarchus labrax in relation to impulsive sound levels caused by pile driving of windmill foundations.

    Directory of Open Access Journals (Sweden)

    Elisabeth Debusschere

    Full Text Available Impact assessments of offshore wind farm installations and operations on the marine fauna are performed in many countries. Yet, only limited quantitative data on the physiological impact of impulsive sounds on (juvenile fishes during pile driving of offshore wind farm foundations are available. Our current knowledge on fish injury and mortality due to pile driving is mainly based on laboratory experiments, in which high-intensity pile driving sounds are generated inside acoustic chambers. To validate these lab results, an in situ field experiment was carried out on board of a pile driving vessel. Juvenile European sea bass (Dicentrarchus labrax of 68 and 115 days post hatching were exposed to pile-driving sounds as close as 45 m from the actual pile driving activity. Fish were exposed to strikes with a sound exposure level between 181 and 188 dB re 1 µPa².s. The number of strikes ranged from 1739 to 3067, resulting in a cumulative sound exposure level between 215 and 222 dB re 1 µPa².s. Control treatments consisted of fish not exposed to pile driving sounds. No differences in immediate mortality were found between exposed and control fish groups. Also no differences were noted in the delayed mortality up to 14 days after exposure between both groups. Our in situ experiments largely confirm the mortality results of the lab experiments found in other studies.

  16. 17β-Estradiol induces changes in cytokine levels in head kidney and blood of juvenile sea bass (Dicentrarchus labrax, L., 1758).

    Science.gov (United States)

    Seemann, Frauke; Knigge, Thomas; Rocher, Béatrice; Minier, Christophe; Monsinjon, Tiphaine

    2013-01-01

    The cytokine network is involved in the immune system communication. As estrogens influence the cytokine expression in mammals, this study investigated the impact of exogenous estrogenic pollutants on selected cytokines in Dicentrarchus labrax. The gene expression of Interleukin 6, Tumour Necrosis Factor α, Transforming Growth Factor β1 and Interleukin 1β was assessed and accomplished with protein measurements in the blood for the last two. Impacts through 17β-estradiol mainly occurred at the beginning of organ regionalisation, thus falling together with a developmentally induced increase of Interleukin 1β and Tumour Necrosis Factor α gene expression in 102 dph fish. 17β-estradiol depressed this modification after 35 days of exposure and the cytokine gene expression tended to be generally down-regulated independently of the 17β-estradiol concentrations after 56 days of exposure. This impact was confirmed at the protein level, showing that 17β-estradiol affects the fine control of the cytokine network in sea bass. PMID:23602341

  17. Biological effects of diethylene glycol (DEG) and produced waters (PWs) released from offshore activities: A multi-biomarker approach with the sea bass Dicentrarchus labrax

    Energy Technology Data Exchange (ETDEWEB)

    Stefania, Gorbi; Maura, Benedetti [Dipartimento di Biochimica, Biologia e Genetica, Universita Politecnica delle Marche, Via Ranieri, Monte d' Ago, 60121 Ancona (Italy); Claudia, Virno Lamberti [Istituto Superiore per la Ricerca e la Protezione Ambientale (ISPRA), Via di Casalotti 300 Roma (Italy); Barbara, Pisanelli [Dipartimento di Biochimica, Biologia e Genetica, Universita Politecnica delle Marche, Via Ranieri, Monte d' Ago, 60121 Ancona (Italy); Ginevra, Moltedo [Istituto Superiore per la Ricerca e la Protezione Ambientale (ISPRA), Via di Casalotti 300 Roma (Italy); Francesco, Regoli, E-mail: f.regoli@univpm.i [Dipartimento di Biochimica, Biologia e Genetica, Universita Politecnica delle Marche, Via Ranieri, Monte d' Ago, 60121 Ancona (Italy)

    2009-11-15

    Diethylene glycol (DEG) is largely used during oil and gas exploitation by offshore platforms. The aim of this work was to investigate if this compound induces direct molecular/cellular effects in marine organisms, or indirectly modulate those of produced waters (PWs). Sea bass (Dicentrarchus labrax) were exposed to DEG dosed alone or in combination with PWs from an Adriatic platform. A wide array of analysed biomarkers included cytochrome P450-dependent enzymatic activity, bile metabolites, glutathione S-transferases, acetylcholinesterase, peroxisomal proliferation, antioxidant defences (catalase, glutathione reductase, glutathione peroxidases, glutathione), total oxyradical scavenging capacity, malondialdehyde and DNA integrity (single strand breaks and frequency of micronuclei). Results did not reveal marked effects of DEG, while PWs influenced the biotransformation system, the oxidative status and the onset of genotoxic damages. Co-exposures caused only limited differences of biomarker responses at some experimental conditions, overall suggesting a limited biological impact of DEG at levels normally deriving from offshore activities. - A biological risk for marine organisms can be excluded for DEG concentrations as those normally associated to produced waters discharged in the Adriatic Sea.

  18. A model for predicting the quantities of dissolved inorganic nitrogen released in effluents from a sea bass (Dicentrarchus labrax) recirculating water system

    OpenAIRE

    Pagand, Pascal; Blancheton, Jean-Paul; Casellas, Claude

    2000-01-01

    Fish excretions and the transformation of nitrogen by bacteria in the nitrifying biofilter are two of the main sources of dissolved inorganic nitrogen (DIN) in fish farms that use recirculating water systems. In this study, the DIN concentration in an experimental Dicentrarchus labrax aquaculture system was calculated using empirical sub-models for fish growth, ingested food and water replacement. The specific growth rate (SGR) (% day(-1)) and the daily feeding rate (DFR) (% day(-1)) both dep...

  19. Acceleration performance of individual European sea bass Dicentrarchus labrax measured with a sprint performance chamber: comparison with high-speed cinematography and correlates with ecological performance.

    Science.gov (United States)

    Vandamm, Joshua P; Marras, Stefano; Claireaux, Guy; Handelsman, Corey A; Nelson, Jay A

    2012-01-01

    Locomotor performance can influence the ecological and evolutionary success of a species. For fish, favorable outcomes of predator-prey encounters are often presumably due to robust acceleration ability. Although escape-response or "fast-start" studies utilizing high-speed cinematography are prevalent, little is known about the contribution of relative acceleration performance to ecological or evolutionary success in a species. This dearth of knowledge may be due to the time-consuming nature of analyzing film, which imposes a practical limit on sample sizes. Herein, we present a high-throughput potential alternative for measuring fish acceleration performance using a sprint performance chamber (SPC). The acceleration performance of a large number of juvenile European sea bass (Dicentrarchus labrax) from two populations was analyzed. Animals from both hatchery and natural ontogenies were assessed, and animals of known acceleration ability had their ecological performance measured in a mesocosm environment. Individuals from one population also had their acceleration performance assessed by both high-speed cinematography and an SPC. Acceleration performance measured in an SPC was lower than that measured by classical high-speed video techniques. However, short-term repeatability and interindividual variation of acceleration performance were similar between the two techniques, and the SPC recorded higher sprint swimming velocities. Wild fish were quicker to accelerate in an SPC and had significantly greater accelerations than all groups of hatchery-raised fish. Acceleration performance had no significant effect on ecological performance (as assessed through animal growth and survival in the mesocosms). However, it is worth noting that wild animals did survive predation in the mesocosm better than farmed ones. Moreover, the hatchery-originated fish that survived the mesocosm experiment, when no predators were present, displayed significantly increased acceleration

  20. Evaluation of different methods of stunning/killing sea bass (Dicentrarchus labrax) by tissue stress/quality indicators

    OpenAIRE

    Zampacavallo, Giulia; Parisi, Giuliana; Mecatti, Massimo; Lupi, Paola; Giorgi, Gianluca; Poli, Bianca Maria

    2014-01-01

    The aim of the study was to evaluate the effect on the final product quality of certain innovative stunning/killing methods for sea bass as substitutes for the most common methods used by European farmers. The changes in tissue stress/quality parameters were monitored from the first hours after death and during the shelf life of the fish. Two trials were conducted in July and November on n. 231 sea bass stunned/killed by ice-water slurry, by single gas or mixture of gases in ice-water and by ...

  1. A standard experimental diet for the study of fatty acid requirements of weaning and first ongrowing stages of the European sea bass Dicentrarchus labrax L.: comparison of extruded and extruded/coated diets.

    Science.gov (United States)

    Coutteau, P; Van Stappen, G; Sorgeloos, P

    1996-01-01

    The stability of the coated lipid fraction of a standard weaning diet was verified by comparing growth and fatty acid incorporation for European sea bass fed diets with an identical formulation but prepared either by extrusion/coating or extrusion only. For each type of experimental diet, a (n-3) HUFA level of 1 and 2.5% of the dry diet was evaluated. European sea bass Dicentrarchus labrax L. were weaned and consequently reared for 23 days on the experimental diets in a recirculation system. A commercial weaning diet served as a control. Final individual dry weight of sea bass larvae weaned and ongrown for a total period of 30 days on the various diets did not differ significantly. The (n-3) HUFA content of the fish tissue was a reflection of the dietary (n-3) HUFA requirements of European sea bass during and immediately after weaning did not exceed 1% of the dry diet. The combined technique of cooking-extrusion of a basal diet followed by coating of a lipid emulsion proved to be a valid technique to prepare diets for studying quantitative fatty acid requirements of weaning and first ongrowing stages of marine fish. A standard diet with open formulation is proposed.

  2. Consumer preferences regarding the introduction of new organic products. The case of the Mediterranean sea bass (Dicentrarchus labrax) in Italy.

    Science.gov (United States)

    Mauracher, C; Tempesta, T; Vecchiato, D

    2013-04-01

    The introduction of new products on the market poses several challenges; in particular, whether the characteristics of the proposed product will be judged positively by potential consumers. This paper analyses the preferences of consumers regarding the introduction on the Italian market of a new product: organic Mediterranean sea bass. The aim of this study is to assess the importance given by consumers to four main characteristics of sea bass (country of origin, size, production method - organic or conventional - and price) so as to be able to formulate marketing strategies. We applied a choice experiment (CE) in order to define not only the ordinal ranking of preferences but also the willingness to pay (WTP) for the key characteristics of the newly-introduced product. We found that consumers show a higher WTP for the sea bass country of origin than for the breeding method used. Our results suggest that while organic aquaculture might be a new and important strategy for diversification, if suitable communication, either from a public policy or commercial perspective, and labelling/certification are not taken into consideration, the added value of the production method might not be perceived by the final consumers. PMID:23268110

  3. Leptin receptor gene in the European sea bass (Dicentrarchus labrax): Cloning, phylogeny, tissue distribution and neuroanatomical organization.

    Science.gov (United States)

    Escobar, Sebastián; Rocha, Ana; Felip, Alicia; Carrillo, Manuel; Zanuy, Silvia; Kah, Olivier; Servili, Arianna

    2016-04-01

    In this study, we report the cloning of three transcripts for leptin receptor in the European sea bass, a marine teleost of economic interest. The two shortest variants, generated by different splice sites, encode all functional extracellular and intracellular domains but missed the transmembrane domain. The resulting proteins are therefore potential soluble binding proteins for leptin. The longest transcript (3605bp), termed sblepr, includes all the essential domains for binding and transduction of the signal. Thus, it is proposed as the ortholog for the human LEPR gene, the main responsible for leptin signaling. Phylogenetic analysis shows the sblepr clustered within the teleost leptin receptor group in 100% of the bootstrap replicates. The neuroanatomical localization of sblepr expressing cells has been assessed by in situ hybridization in brains of sea bass of both sexes during their first sexual maturation. At histological level, the distribution pattern of sblepr expressing cells in the brain shows no clear differences regarding sex or reproductive season. Transcripts of the sblepr have a widespread distribution throughout the forebrain and midbrain until the caudal portion of the hypothalamus. A high hybridization signal is detected in the telencephalon, preoptic area, medial basal and caudal hypothalamus and in the pituitary gland. In a more caudal region, sblepr expressing cells are identified in the longitudinal torus. The expression pattern observed for sblepr suggests that in sea bass, leptin is very likely to be involved in the control of food intake, energy reserves and reproduction. PMID:26979276

  4. Consumer preferences regarding the introduction of new organic products. The case of the Mediterranean sea bass (Dicentrarchus labrax) in Italy.

    Science.gov (United States)

    Mauracher, C; Tempesta, T; Vecchiato, D

    2013-04-01

    The introduction of new products on the market poses several challenges; in particular, whether the characteristics of the proposed product will be judged positively by potential consumers. This paper analyses the preferences of consumers regarding the introduction on the Italian market of a new product: organic Mediterranean sea bass. The aim of this study is to assess the importance given by consumers to four main characteristics of sea bass (country of origin, size, production method - organic or conventional - and price) so as to be able to formulate marketing strategies. We applied a choice experiment (CE) in order to define not only the ordinal ranking of preferences but also the willingness to pay (WTP) for the key characteristics of the newly-introduced product. We found that consumers show a higher WTP for the sea bass country of origin than for the breeding method used. Our results suggest that while organic aquaculture might be a new and important strategy for diversification, if suitable communication, either from a public policy or commercial perspective, and labelling/certification are not taken into consideration, the added value of the production method might not be perceived by the final consumers.

  5. Growth parameters in European sea bass (Dicentrarchus labraxL.: effects of live weight and water temperature

    Directory of Open Access Journals (Sweden)

    Rodolfo Ballestrazzi

    2010-01-01

    Full Text Available The voluntary feed intake (VFI and growth rate of sea bass were studied for 147 days, based on different starting live  weights, under natural photoperiods and varied water temperatures. Sea bass (n = 720 were divided into five weight  classes (60-70, 90-110, 130-150, 160-180 and 230-250 g and distributed among 20 tanks. Seven different water tem-  peratures were compared: 13, 16, 19, 22, 25, 28 and 31 °C. A commercial extruded diet (N x 6.25, 43.7% DM; crude  fat, 25.7% DM was used. The trial was performed in a closed-circuit plant, with a daily water turnover rate of 10%. Each  tank was fitted with an apparatus for collecting the uneaten food. Automatic feeders distributed equal amounts of food  for ten meals per day. The daily food allowance was adjusted, based on fish biomass, to permit ad libitum feeding and  to leave approximately 20% uneaten. Fish were exposed to natural photoperiod (March-July. Non-linear regression  equations were used to calculate the best-fitting curves for the data. 

  6. Effects of pressure-shift freezing and pressure-assisted thawing on sea bass (Dicentrarchus labrax) quality.

    Science.gov (United States)

    Tironi, V; LeBail, A; de Lamballerie, M

    2007-09-01

    Effects of pressure-shift freezing and/or pressure-assisted thawing on the quality of sea bass muscle were evaluated and compared with conventional (air-blast) frozen and thawed samples. Microstructural analysis showed a marked decrease of muscle cell damage for pressure-assisted frozen samples. According to differential scanning calorimetry (DSC), protein extractability, and SDS-PAGE results, high-pressure treatment (200 MPa) produced a partial denaturation with aggregation and insolubilization of the myosin, as well as alterations of the sarcoplasmic proteins. Only small differences between high-pressure processes (freezing or/and thawing) were registered. High-pressure-treated systems led to a decrease of water holding capacity but differences between high-pressure and conventional methods disappeared after cooking. Muscle color showed important alterations due to high-pressure treatments (increasing L* and b*).

  7. Effect of dietary fat level on carcass traits and flesh quality of European Sea Bass (Dicentrarchus labrax from mariculture

    Directory of Open Access Journals (Sweden)

    Severino Segato

    2010-01-01

    Full Text Available The study aimed at evaluating the effect of the reduction of dietary fat on juvenile European sea bass nutritional value and quality traits. Fish were reared in floating cages (Trieste Gulf, Italy from July (11 to October (10. Two isoproteic diets were compared: LF (low fat, EE = 19.4% vs. HF (high fat, EE = 24.6%. No significantly different growth performance was observed. LF diet-fed fish were characterized by the reduction of celomatic fat (not edible fraction and by the increase in dressing percentage. The tested dietary fat level also affected both fillet and epiaxial white muscle proximate composition, resulting in a significantly lower fillet lipid concentration in LF diet-fed fish. Dietary treatment influenced cooked fillet colour and texture probably as a consequence of the different intramuscular fat deposition. Fillet from HF-fed fish, in fact, presented higher lightness (L* value and lower instrumental strengthness.

  8. Evaluation of different methods of stunning/killing sea bass (Dicentrarchus labrax) by tissue stress/quality indicators.

    Science.gov (United States)

    Zampacavallo, Giulia; Parisi, Giuliana; Mecatti, Massimo; Lupi, Paola; Giorgi, Gianluca; Poli, Bianca Maria

    2015-05-01

    The aim of the study was to evaluate the effect on the final product quality of certain innovative stunning/killing methods for sea bass as substitutes for the most common methods used by European farmers. The changes in tissue stress/quality parameters were monitored from the first hours after death and during the shelf life of the fish. Two trials were conducted in July and November on n. 231 sea bass stunned/killed by ice-water slurry, by single gas or mixture of gases in ice-water and by single- or two-stage electrical stunning/killing methods. Behavioural responses, stun/death time, rigor index, muscular and ocular pH, lactic acid, ATP and catabolites at death and within the 24 h after death were determined. In the November trial, the sensorial evaluation, rigor index, IMP, inosine, hypoxanthine, and K1 values were also evaluated during refrigerated storage until spoilage. The stunning/killing in ice-water appeared to induce low effects on the analysed parameters and preserve a good product quality as indicated by the highest pH and ATP values at death, the delayed full rigor onset and the 1 day longer shelf life (14 days) in comparison with the single- or two-stage electrical stunning/killing. The gas mixture addition provided a 40 % shortening of the time to obtain stunning/killing and 14 days of shelf life. The actual level of quality loss with the different killing conditions and the actual impact of a significant shortage of rigor mortis onset and pH drop on the possible pre-rigor filleting remain to be studied in depth. PMID:25892757

  9. First Insight into Exploration and Cognition in Wild Caught and Domesticated Sea Bass (Dicentrarchus labrax in a Maze.

    Directory of Open Access Journals (Sweden)

    David Benhaïm

    Full Text Available European sea bass aquaculture is so recent that very little is known on the effects of the early steps of its domestication. Behavioural parameters are sensitive indicators of the domestication process since they are generally impacted as soon as the first generation. The present work compared wild-caught and domesticated sea bass juvenile swimming activity, exploration and ability to learn to discriminate between two 2-D objects associated to a simple spatial task that enabled the tested individual to visually interact with an unfamiliar congener (the reward located behind a transparent wall at the end of one of the two arms of a maze. Ten fish from each origin were individually tested 3 times in a row during 3 days (9 trials in total. Fish were placed in a start box closed by a transparent wall located in front of two 2-D objects. Fish were filmed during 10 min after the removal of the start box wall. Different swimming variables including angular velocity, total distance travelled and velocity mean, were analyzed from videos as well as the time spent in each of 6 virtual zones including the reward zone near the congener (Cong and the zone opposite to the reward zone (OpCong. Two learning criteria were chosen: the number of successful turns and time to reach Cong. Behavioural differences were found between domesticated and wild fish. Angular velocity was higher in wild fish while the distance travelled and the velocity mean were higher in domesticated ones. Wild and domesticated fish spent most of the time in Cong and in OpCong. No differences were seen in learning ability between wild and domesticated fish. However, our findings for learning require confirmation by further studies with larger numbers of learning sessions and experiments designed to minimise stress. This study therefore demonstrated an impact of domestication on swimming behaviour but not on spatial learning.

  10. Production and characterization of recombinantly derived peptides and antibodies for accurate determinations of somatolactin, growth hormone and insulin-like growth factor-I in European sea bass (Dicentrarchus labrax).

    Science.gov (United States)

    de Celis, S Vega-Rubín; Gómez-Requeni, P; Pérez-Sánchez, J

    2004-12-01

    A specific radioimmunoassay (RIA) for European sea bass (Dicentrarchus labrax) growth hormone (GH) was developed and validated. For this purpose, a stable source of GH was produced by means of recombinant DNA technology in a bacteria system. The identity of the purified protein (ion exchange chromatography) was demonstrated by Western blot and a specific GH antiserum was raised in rabbit. In Western blot and RIA system, this antiserum recognized specifically native and recombinant GH, and it did not cross-react with fish prolactin (PRL) and somatolactin (SL). In a similar way, a specific polyclonal antiserum against the now available recombinant European sea bass SL was raised and used in the RIA system to a sensitivity of 0.3 ng/ml (90% of binding of tracer). Further, European sea bass insulin-like growth factor-I (IGF-I) was cloned and sequenced, and its high degree of identity with IGF-I peptides of barramundi, tuna, and sparid fish allowed the use of a commercial IGF-I RIA based on barramundi IGF-I antiserum. These assay tools assisted for the first time accurate determinations of SL and GH-IGF-I axis activity in a fish species of the Moronidae family. Data values were compared to those found with gilthead sea bream (Sparus aurata), which is currently used as a Mediterranean fish model for growth endocrinology studies. As a characteristic feature, the average concentration year round of circulating GH in growing mature males of European sea bass was higher than in gilthead sea bream. By contrast, the average concentration of circulating SL was lower. Concerning to circulating concentration of IGF-I, the measured plasma values for a given growth rate were also lower in European sea bass. These findings are discussed on the basis of a different energy status that might allowed a reduced but more continuous growth in European sea bass. PMID:15560873

  11. Gonadotropin Inhibitory Hormone Down-Regulates the Brain-Pituitary Reproductive Axis of Male European Sea Bass (Dicentrarchus labrax).

    Science.gov (United States)

    Paullada-Salmerón, José A; Cowan, Mairi; Aliaga-Guerrero, María; Morano, Francesca; Zanuy, Silvia; Muñoz-Cueto, José A

    2016-06-01

    Gonadotropin-inhibitory hormone (GnIH) inhibits gonadotropin synthesis and release from the pituitary of birds and mammals. However, the physiological role of orthologous GnIH peptides on the reproductive axis of fish is still uncertain, and their actions on the main neuroendocrine systems controlling reproduction (i.e., GnRHs, kisspeptins) have received little attention. In a recent study performed in the European sea bass, we cloned a cDNA encoding a precursor polypeptide that contained C-terminal MPMRFamide (sbGnIH-1) and MPQRFamide (sbGnIH-2) peptide sequences, developed a specific antiserum against sbGnIH-2, and characterized its central and pituitary GnIH projections in this species. In this study, we analyzed the effects of intracerebroventricular injection of sbGnIH-1 and sbGnIH-2 on brain and pituitary expression of reproductive hormone genes (gnrh1, gnrh2, gnrh3, kiss1, kiss2, gnih, lhbeta, fshbeta), and their receptors (gnrhr II-1a, gnrhr II-2b, kiss1r, kiss2r, and gnihr) as well as on plasma Fsh and Lh levels. In addition, we determined the effects of GnIH on pituitary somatotropin (Gh) expression. The results obtained revealed the inhibitory role of sbGnIH-2 on brain gnrh2, kiss1, kiss2, kiss1r, gnih, and gnihr transcripts and on pituitary fshbeta, lhbeta, gh, and gnrhr-II-1a expression, whereas sbGnIH-1 only down-regulated brain gnrh1 expression. However, at different doses, central administration of both sbGnIH-1 and sbGnIH-2 decreased Lh plasma levels. Our work represents the first study reporting the effects of centrally administered GnIH in fish and provides evidence of the differential actions of sbGnIH-1 and sbGnIH-2 on the reproductive axis of sea bass, the main inhibitory role being exerted by the sbGnIH-2 peptide. PMID:26984999

  12. Effects of electro-fishing on galvano-taxis and carcass quality characteristics in sea bass (Dicentrarchus labrax

    Directory of Open Access Journals (Sweden)

    Edo D’Agaro

    2010-01-01

    Full Text Available The aim of this study was to investigate the effects of electro-fishing in sea water. We evaluated the feasibility of an electro-fishing system using numerical simulations for laboratory tanks and the open sea and performing a laboratory experiment. A non-homogeneous bi-dimensional electric-field model for marine water and fish based on discrete formulation of electro-magnetic field equations was developed using GAME (geometric approach for Maxwell equations software. Voltage gradients inside the fish and close to the body were determined. Re- sults showed that fish in the open sea and in groups had greater internal voltage differences than did fish in tanks and single fish. Sea bass (length:10 and 30 cm were exposed in laboratory tanks to pulsed direct current (PDC, 25-125 Hz and duty cycle (5-40%. We measured the electro-taxis and tetanus thresholds after electrical exposure. It is significant that these values decreased with increasing the size of fish. No differences were found after electro-fishing on overall appearance, internal and external haemorrhage, standard freshness scoring techniques and carcass quality characteristics

  13. Nutritional Status as the Key Modulator of Antioxidant Responses Induced by High Environmental Ammonia and Salinity Stress in European Sea Bass (Dicentrarchus labrax.

    Directory of Open Access Journals (Sweden)

    Amit Kumar Sinha

    Full Text Available Salinity fluctuation is one of the main factors affecting the overall fitness of marine fish. In addition, water borne ammonia may occur simultaneously with salinity stress. Additionally, under such stressful circumstances, fish may encounter food deprivation. The physiological and ion-osmo regulatory adaptive capacities to cope with all these stressors alone or in combination are extensively addressed in fish. To date, studies revealing the modulation of antioxidant potential as compensatory response to multiple stressors are rather lacking. Therefore, the present work evaluated the individual and combined effects of salinity challenge, ammonia toxicity and nutritional status on oxidative stress and antioxidant status in a marine teleost, European sea bass (Dicentrarchus labrax. Fish were acclimated to normal seawater (32 ppt, to brackish water (20 ppt and 10 ppt and to hypo-saline water (2.5 ppt. Following acclimation to different salinities for two weeks, fish were exposed to high environmental ammonia (HEA, 20 mg/L representing 50% of 96h LC50 value for ammonia for 12 h, 48 h, 84 h and 180 h, and were either fed (2% body weight or fasted (unfed for 7 days prior to HEA exposure. Results show that in response to decreasing salinities, oxidative stress indices such as xanthine oxidase activity, levels of hydrogen peroxide (H2O2 and lipid peroxidation (malondialdehyde, MDA increased in the hepatic tissue of fasted fish but remained unaffected in fed fish. HEA exposure at normal salinity (32 ppt and at reduced salinities (20 ppt and 10 ppt increased ammonia accumulation significantly (84 h-180 h in both feeding regimes which was associated with an increment of H2O2 and MDA contents. Unlike in fasted fish, H2O2 and MDA levels in fed fish were restored to control levels (84 h-180 h; with a concomitant increase in superoxide dismutase (SOD, catalase (CAT, components of the glutathione redox cycle (reduced glutathione, glutathione peroxidase and

  14. Cloning and expression analysis of myostatin, fibroblast growth factor 6, insulin-like growth factor I and II in liver and muscle of sea bass (Dicentrarchus labrax, L. during long-term fasting and refeeding

    Directory of Open Access Journals (Sweden)

    M. Saroglia

    2010-04-01

    Full Text Available The exceptionally fast growth that fish experience after periods of fasting has been called “compensatory growth”. This phenomenon has been studied in intensive aquaculture as a means of enhancing growth rates, but the mechanisms by which food intake activates an increase in somatic growth, and especially in muscle growth, are complex and not yet fully understood. In the present paper, we describe the molecular cloning and sequencing of sea bass (Dicentrarchus labrax myostatin (MSTN and fibroblast growth factor 6 (FGF6, which have been shown to be major genetic determinants of skeletal muscle growth, together with insulin-like growth factor I (IGFI and IGF-II, which are potent mitogens known to play important roles in growth and development. We then report the pattern of expression of the four aforementioned genes, in liver and myotomal muscle in response to prolonged fasting and refeeding. Nutritional status significantly influenced the expression of IGF-I, IGF-II and MSTN, whereas the muscular FGF6 expression levels were not affected by the feeding status of the animals. Taken together these data indicate that IGF-I, IGF-II and MSTN are involved in the sea bass muscle compensatory growth induced by refeeding, whereas FGF6 probably has not a role in this phenomenon.

  15. Virgibacillus proomii and Bacillus mojavensis as probiotics in sea bass (Dicentrarchus labrax) larvae: effects on growth performance and digestive enzyme activities.

    Science.gov (United States)

    Hamza, Ahlem; Fdhila, Kais; Zouiten, Dora; Masmoudi, Ahmed Sleheddine

    2016-04-01

    This study examined the effects of two probiotics (Virgibacillus proomii and Bacillus mojavensis) on the digestive enzyme activity, survival and growth of Dicentrarchus labrax at various ontogenetic stages in three separate experiments. These probiotics were incorporated as single or mixed into fish feed for a period of 60 days. The growth parameters, proximate composition of whole body, digestive enzymes and gut microbiology were monitored at regular. The increments in length and weight and the survival were significantly higher (P 0.05) in body ash and protein content and in the specific activity of phosphatase alkaline and amylase in the digestive tract of all the fishes. V. proomii and B. mojavensis persisted in the fish intestine and in the feed in high numbers during the feeding period (group 1: 5.8 × 10(4) CFU/ml, group 2: 9.6 × 10(4) CFU/ml, and group 3: 9.8 × 10(4) CFU/ml day 60). The two probiotics V. proomii and B. mojavensis were adequate for improved growth performance and survival and for healthy gut microenvironment of the host. PMID:26520833

  16. Effects of Offshore Wind Farms on the Early Life Stages of Dicentrarchus labrax.

    Science.gov (United States)

    Debusschere, Elisabeth; De Coensel, Bert; Vandendriessche, Sofie; Botteldooren, Dick; Hostens, Kris; Vincx, Magda; Degraer, Steven

    2016-01-01

    Anthropogenically generated underwater noise in the marine environment is ubiquitous, comprising both intense impulse and continuous noise. The installation of offshore wind farms across the North Sea has triggered a range of ecological questions regarding the impact of anthropogenically produced underwater noise on marine wildlife. Our interest is on the impact on the "passive drifters," i.e., the early life stages of fish that form the basis of fish populations and are an important prey for pelagic predators. This study deals with the impact of pile driving and operational noise generated at offshore wind farms on Dicentrarchus labrax (sea bass) larvae. PMID:26610960

  17. Studie van de biologie en de kweek van zeebaars Dicentrarchus labrax (L.) in intensieve aquacultuurcondities

    OpenAIRE

    Verdonck, W.

    1989-01-01

    In Europe. the interest in sea bass (Dicentrarchus labrax) as a culture fish is fast increasing. This results in a rapid expansion of sea bass culture and of that of other marine fish species. such as seabream (Sparus aurata) and turbot (Scophtalmus maximus). In 1983 a research project was started, with the aim of culturing sea bass in the thermal effluents of the nuclear power station at Doel. In this way the possibility of producing valuable proteins is created while at the same time therma...

  18. Aquaculture et ichtyoparasitologie: action in vitro du Nitroxinil© (Antihelminthique) sur Diplectanum aequans , ectoparasite branchial du bar Dicentrarchus labrax

    OpenAIRE

    Silan, Patrick; Birgi, Emile; Louis, Christophe; Clota,, Frédéric; Mathieu, André; Giral, Louis

    1996-01-01

    International audience The sea bass Dicentrarchus labrax is subject to intensive rearing. Several monogeneans, gill ectoparasites with a direct biological cycle, parasitized this Teleostei. Under certain conditions of host density, populations of these platyhelminths can increase and consequently induce important mortality. It's especially the case of Diplectanum aequans, a monopisthocotylea against which no treatment is known. In vitro effects of nitroxinil© (or nitroxynil©), an anthelmin...

  19. Combination effects of nano-TiO2 and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on biotransformation gene expression in the liver of European sea bass Dicentrarchus labrax.

    Science.gov (United States)

    Vannuccini, Maria Luisa; Grassi, Giacomo; Leaver, Michael J; Corsi, Ilaria

    2015-01-01

    The aim of present study was to investigate the influence of titanium dioxide nanoparticles (nano-TiO2, Aeroxide® P25) on 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) dependent biotransformation gene expression in liver of juvenile European sea bass Dicentrarchus labrax. An in vivo 7day waterborne exposure was performed with nano-TiO2 (1mg/L) and 2,3,7,8-TCDD (46pg/L), singly and in combination. The mRNA expression of aryl hydrocarbon receptor repressor (Ahrr), estrogen receptor (erβ2), ABC transport proteins as Abcb1, Abcc1-c2-g2, cytochrome P450 (cyp1a), glutathione-s-transferase (gsta), glutathione reductase (gr) and engulfment and motility (ELMO) domain-containing protein 2 (elmod2) was investigated. Ahrr, erβ2, abcc1 and abcg2 resulted down-regulated with respect to controls in all experimental groups. Co-exposure to nano-TiO2 and 2,3,7,8-TCDD caused a further significant down regulation of ahrr, erβ2, Abcb1 and Abcc2 compared to single chemical exposure (nano-TiO2 or 2,3,7,8-TCDD alone). No effects were observed for 2,3,7,8-TCDD and nano-TiO2 alone in abcb1 gene, while abcc2 was down-regulated by nano-TiO2 alone. Cyp1a, gst and elmod2 genes were up-regulated by 2,3,7,8-TCDD and to a similar extent after co-exposure. Overall the results indicate that nano-TiO2 is unlikely to interfere with 2,3,7,8-TCDD-dependent biotransformation gene expression in the liver of European sea bass, although the effects of co-exposure observed in ABC transport mRNAs might suggest an impact on xenobiotic metabolite disposition and transport in European sea bass liver. PMID:26235595

  20. Effects of the total replacement of fish-based diet with plant-based diet on the hepatic transcriptome of two European sea bass (Dicentrarchus labrax half-sibfamilies showing different growth rates with the plant-based diet

    Directory of Open Access Journals (Sweden)

    Geay Florian

    2011-10-01

    Full Text Available Abstract Background Efforts towards utilisation of diets without fish meal (FM or fish oil (FO in finfish aquaculture have been being made for more than two decades. Metabolic responses to substitution of fishery products have been shown to impact growth performance and immune system of fish as well as their subsequent nutritional value, particularly in marine fish species, which exhibit low capacity for biosynthesis of long-chain poly-unsaturated fatty acids (LC-PUFA. The main objective of the present study was to analyse the effects of a plant-based diet on the hepatic transcriptome of European sea bass (Dicentrarchus labrax. Results We report the first results obtained using a transcriptomic approach on the liver of two half-sibfamilies of the European sea bass that exhibit similar growth rates when fed a fish-based diet (FD, but significantly different growth rates when fed an all-plant diet (VD. Overall gene expression was analysed using oligo DNA microarrays (GPL9663. Statistical analysis identified 582 unique annotated genes differentially expressed between groups of fish fed the two diets, 199 genes regulated by genetic factors, and 72 genes that exhibited diet-family interactions. The expression of several genes involved in the LC-PUFA and cholesterol biosynthetic pathways was found to be up-regulated in fish fed VD, suggesting a stimulation of the lipogenic pathways. No significant diet-family interaction for the regulation of LC-PUFA biosynthesis pathways could be detected by microarray analysis. This result was in agreement with LC-PUFA profiles, which were found to be similar in the flesh of the two half-sibfamilies. In addition, the combination of our transcriptomic data with an analysis of plasmatic immune parameters revealed a stimulation of complement activity associated with an immunodeficiency in the fish fed VD, and different inflammatory status between the two half-sibfamilies. Biological processes related to protein

  1. Study of endocrine disruption in seabass (Dicentrarchus labrax)

    OpenAIRE

    Schnitzler, Joseph; Koutrakis, Emmanuil; Thomé, Jean-Pierre; Siebert, Ursula; Das, Krishna

    2006-01-01

    Organochlorinated compounds such as polychlorobiphenyls (PCB), dichloro-diphenyl-dichloroethylene (DDE) and dichloro-diphenyl-trichloroethane (DDT) have the characteristic to influence the endocrine system of many fish species and mammals and are thus qualified as endocrine disruptors. The objective of this project aims a better understanding of the effects of these compounds on the thyroid function of European seabass (Dicentrarchus labrax). Thyroid tissue and muscle of 46 seabass from t...

  2. Effects of feeding and hypoxia on cardiac performance and gastrointestinal blood flow during critical speed swimming in the sea bass Dicentrarchus labrax.

    Science.gov (United States)

    Dupont-Prinet, A; Claireaux, G; McKenzie, D J

    2009-10-01

    Previous studies have shown that if European sea bass are exercised after feeding, they can achieve a significantly higher maximum metabolic rate (MMR) than when fasted. They can meet combined metabolic demands of digestion (specific dynamic action, SDA) and maximal aerobic exercise, with no decline in swimming performance. If, however, exposed to mild hypoxia (50% saturation), bass no longer achieve higher MMR after feeding but they swim as well fed as fasted, due to an apparent ability to defer the SDA response. This study explored patterns of cardiac output (Q(A)) and blood flow to the gastrointestinal tract (Q(GI)) associated with the higher MMR after feeding, and with the ability to prioritise swimming in hypoxia. Sea bass (mean mass approximately 325 g, forklength approximately 27 cm) were instrumented with flow probes to measure Q(A) and Q(GI) during an incremental critical swimming speed (U(crit)) protocol in a tunnel respirometer, to compare each animal either fasted or 6h after a meal of fish fillet equal to 3% body mass. Feeding raised oxygen uptake (M(O2)) prior to exercise, an SDA response associated with increased Q(A) (+30%) and Q(GI) (+100%) compared to fasted values. As expected, when exercised the fed bass maintained the SDA load throughout the protocol and achieved 14% higher MMR than when fasted, and the same U(crit) (approximately 100 cm s(-1)). Both fed and fasted bass showed pronounced increases in Q(A) and decreases in Q(GI) during exercise and the higher MMR of fed bass was not associated with higher maximum Q(A) relative to when fasted, or to any differences in Q(GI) at maximum Q(A). In hypoxia prior to exercise, metabolic and cardiac responses to feeding were similar compared to normoxia. Hypoxia caused an almost 60% reduction to MMR and 30% reduction to U(crit), but neither of these traits differed between fed or fasted bass. Despite hypoxic limitations to MMR and U(crit), maximum Q(A) and patterns of Q(GI) during exercise in fasted and

  3. Effects of Sodium Butyrate Treatment on Histone Modifications and the Expression of Genes Related to Epigenetic Regulatory Mechanisms and Immune Response in European Sea Bass (Dicentrarchus Labrax) Fed a Plant-Based Diet

    Science.gov (United States)

    Díaz, Noelia; Rimoldi, Simona; Ceccotti, Chiara; Gliozheni, Emi; Piferrer, Francesc

    2016-01-01

    Bacteria that inhabit the epithelium of the animals’ digestive tract provide the essential biochemical pathways for fermenting otherwise indigestible dietary fibers, leading to the production of short-chain fatty acids (SCFAs). Of the major SCFAs, butyrate has received particular attention due to its numerous positive effects on the health of the intestinal tract and peripheral tissues. The mechanisms of action of this four-carbon chain organic acid are different; many of these are related to its potent regulatory effect on gene expression since butyrate is a histone deacetylase inhibitor that play a predominant role in the epigenetic regulation of gene expression and cell function. In the present work, we investigated in the European sea bass (Dicentrarchus labrax) the effects of butyrate used as a feed additive on fish epigenetics as well as its regulatory role in mucosal protection and immune homeostasis through impact on gene expression. Seven target genes related to inflammatory response and reinforcement of the epithelial defense barrier [tnfα (tumor necrosis factor alpha) il1β, (interleukin 1beta), il-6, il-8, il-10, and muc2 (mucin 2)] and five target genes related to epigenetic modifications [dicer1(double-stranded RNA-specific endoribonuclease), ehmt2 (euchromatic histone-lysine-N-methyltransferase 2), pcgf2 (polycomb group ring finger 2), hdac11 (histone deacetylase-11), and jarid2a (jumonji)] were analyzed in fish intestine and liver. We also investigated the effect of dietary butyrate supplementation on histone acetylation, by performing an immunoblotting analysis on liver core histone extracts. Results of the eight-week-long feeding trial showed no significant differences in weight gain or SGR (specific growth rate) of sea bass that received 0.2% sodium butyrate supplementation in the diet in comparison to control fish that received a diet without Na-butyrate. Dietary butyrate led to a twofold increase in the acetylation level of histone H4 at

  4. Effects of Sodium Butyrate Treatment on Histone Modifications and the Expression of Genes Related to Epigenetic Regulatory Mechanisms and Immune Response in European Sea Bass (Dicentrarchus Labrax) Fed a Plant-Based Diet.

    Science.gov (United States)

    Terova, Genciana; Díaz, Noelia; Rimoldi, Simona; Ceccotti, Chiara; Gliozheni, Emi; Piferrer, Francesc

    2016-01-01

    Bacteria that inhabit the epithelium of the animals' digestive tract provide the essential biochemical pathways for fermenting otherwise indigestible dietary fibers, leading to the production of short-chain fatty acids (SCFAs). Of the major SCFAs, butyrate has received particular attention due to its numerous positive effects on the health of the intestinal tract and peripheral tissues. The mechanisms of action of this four-carbon chain organic acid are different; many of these are related to its potent regulatory effect on gene expression since butyrate is a histone deacetylase inhibitor that play a predominant role in the epigenetic regulation of gene expression and cell function. In the present work, we investigated in the European sea bass (Dicentrarchus labrax) the effects of butyrate used as a feed additive on fish epigenetics as well as its regulatory role in mucosal protection and immune homeostasis through impact on gene expression. Seven target genes related to inflammatory response and reinforcement of the epithelial defense barrier [tnfα (tumor necrosis factor alpha) il1β, (interleukin 1beta), il-6, il-8, il-10, and muc2 (mucin 2)] and five target genes related to epigenetic modifications [dicer1(double-stranded RNA-specific endoribonuclease), ehmt2 (euchromatic histone-lysine-N-methyltransferase 2), pcgf2 (polycomb group ring finger 2), hdac11 (histone deacetylase-11), and jarid2a (jumonji)] were analyzed in fish intestine and liver. We also investigated the effect of dietary butyrate supplementation on histone acetylation, by performing an immunoblotting analysis on liver core histone extracts. Results of the eight-week-long feeding trial showed no significant differences in weight gain or SGR (specific growth rate) of sea bass that received 0.2% sodium butyrate supplementation in the diet in comparison to control fish that received a diet without Na-butyrate. Dietary butyrate led to a twofold increase in the acetylation level of histone H4 at

  5. Antioxidant, antimicrobial and sensorial effects of Rosemary (Rosmarinus officinalis L.) and Sage (Salvia officinalis L.) on Sea bass (Dicentrarchus labrax L.) fillets

    OpenAIRE

    Can Altınelataman; Duygu Kışla; Berna Kılınç; Evren Burcu Şen Yılmaz; Aslı Cadun Yünlü; Tolga Dinçer; Ufuk Çelik

    2015-01-01

    In this study, effects of sum of vapor distillate and water phase extract of rosemary and sage leaves on sea bass fillets quality, were investigated. For this purpose, effective solution was obtained with same amounts of these plants, cooled down to room temperature, applied to fillets and samples were stored in refrigerated conditions during 13 days. In monitoring period, pH, TVB-N (Total Viable Base –Nitrogen), TBA (Tiobarbutiric acid ), color, textural, microbiological and sensorial analys...

  6. Effects of multiple freezing (-18 ± 2 °C) and microwave thawing cycles on the quality changes of sea bass (Dicentrarchus labrax).

    Science.gov (United States)

    Baygar, Taçnur; Alparslan, Yunus

    2015-06-01

    Sensory, chemical [pH, total volatile basic nitrogen (TVB-N), trimethylamine (TMA-N), thiobarbituric acid (TBA)] and composition (crude protein and crude lipid) analyses were carried out on whole, gutted and fillets of seabass that were frozen (-18 ± 2 °C) and thawed more than once in microwave. It was detected that the sensorial acceptability values decreased certainly for all fish samples (whole, gutted and fillets of sea bass) when compared with control group. While chemical (pH, TVB-N, TMA-N, TBA) values increased, crude protein and crude lipid values decreased after the multiple freezing and thawing cycles under microwave. The obtained results offered that thawing by microwave is not appropriate sea bass because of the undesirable cooking effect of microwave to the some parts of fish such as tail, eye and fins. Also skin dryness, moisture losses in eye fluids and textural deteriorations were observed. Significant differences (P sea bass groups thawed in microwave conditions.

  7. Is the kisspeptin system involved in responses to food restriction in order to preserve reproduction in pubertal male sea bass (Dicentrarchus labrax)?

    Science.gov (United States)

    Escobar, Sebastián; Felip, Alicia; Zanuy, Silvia; Carrillo, Manuel

    2016-09-01

    Previous works on European sea bass have determined that long-term exposure to restrictive feeding diets alters the rhythms of some reproductive/metabolic hormones, delaying maturation and increasing apoptosis during gametogenesis. However, exactly how these diets affect key genes and hormones on the brain-pituitary-gonad (BPG) axis to trigger puberty is still largely unknown. We may hypothesize that all these signals could be integrated, at least in part, by the kisspeptin system. In order to capture a glimpse of these regulatory mechanisms, kiss1 and kiss2 mRNA expression levels and those of their kiss receptors (kiss1r, kiss2r) were analyzed in different areas of the brain and in the pituitary of pubertal male sea bass during gametogenesis. Furthermore, other reproductive hormones and factors as well as the percentage of males showing full spermiation were also analyzed. Treated fish fed maintenance diets provided evidence of overexpression of the kisspeptin system in the main hypophysiotropic regions of the brain throughout the entire sexual cycle. Conversely, Gnrh1 and gonadotropin pituitary content and plasma sexual steroid levels were downregulated, except for Fsh levels, which were shown to increase during spermiation. Treated fish exhibited lower rates of spermiation as compared to control group and a delay in its accomplishment. These results demonstrate how the kisspeptin system and plasma Fsh levels are differentially affected by maintenance diets, causing a retardation, but not a full blockage of the reproductive process in the teleost fish European sea bass. This suggests that a hormonal adaptive strategy may be operating in order to preserve reproductive function in this species. PMID:27164487

  8. Impact of acute stress on antimicrobial polypeptides mRNA copy number in several tissues of marine sea bass (Dicentrarchus labrax

    Directory of Open Access Journals (Sweden)

    Terova Genciana

    2011-12-01

    Full Text Available Abstract Background In comparison to higher vertebrates, fish are thought to rely heavily on innate immune system for initial protection against pathogen invasion because their acquired immune system displays a considerably poor immunological memory, and short-lived secondary response. The endogenous antimicrobial polypeptides (AMPPs directly and rapidly killing pathogens such as bacteria, fungi, parasites, and viruses are included within the realm of innate defenses. In addition to piscidins, AMPPs that in recent years have been shown to be commonly linked to innate defense, are histones and their polypeptide fragments, and peptides derived from the respiratory protein hemoglobin. There is evidence that a number of stresses lead to significant regulation of AMPPs and thus their monitoring could be a highly sensitive measure of health status and risk of an infectious disease outbreak, which is a major impediment to the continued success of virtually all aquaculture enterprises and is often the most significant cause of economic losses. Results We firstly isolated and deposited in Genbank database the cDNA sequences encoding for hemoglobin-β-like protein (Hb-LP [GeneBank: JN410659], H2B histone-like protein 1 (HLP1 GenBank: JN410660], and HLP2 [GenBank: JN410661]. The "de novo" prediction of the three-dimensional structures for each protein is presented. Phylogenetic trees were constructed on Hb-LP, HLP1, and HLP2 sequences of sea bass and those of other teleost, avian, reptiles, amphibian and mammalian species. We then used real time RT-PCR technology to monitor for the first time in sea bass, dynamic changes in mRNA copy number of Hb-LP, HLP1, HLP2, and dicentracin in gills, skin, eyes, stomach and proximal intestine in response to acute crowding/confinement stress. We showed that acute crowding stress induces an increase in the expression levels of the aforementioned genes, in gills and skin of sea bass, but not in other tissues, and that

  9. Effects of fish oil replacement by vegetable oil blend on digestive enzymes and tissue histomorphology of European sea bass (Dicentrarchus labrax) juveniles.

    Science.gov (United States)

    Castro, Carolina; Couto, Ana; Pérez-Jiménez, Amalia; Serra, Cláudia R; Díaz-Rosales, Patricia; Fernandes, Rui; Corraze, Geneviève; Panserat, Stéphane; Oliva-Teles, Aires

    2016-02-01

    The impact of replacing circa 70% fish oil (FO) by a vegetable oil (VO) blend (rapeseed, linseed, palm oils; 20:50:30) in diets for European sea bass juveniles (IBW 96 ± 0.8 g) was evaluated in terms of activities of digestive enzymes (amylase, lipase, alkaline phosphatase, trypsin and total alkaline proteases) in the anterior (AI) and posterior (PI) intestine and tissue morphology (pyloric caeca-PC, AI, PI, distal intestine-DI and liver). For that purpose, fish were fed the experimental diets for 36 days and then liver and intestine were sampled at 2, 6 and 24 h after the last meal. Alkaline protease characterization was also done in AI and PI at 6 h post-feeding. Dietary VO promoted higher alkaline phosphatase activity at 2 h post-feeding in the AI and at all sampling points in the PI. Total alkaline protease activity was higher at 6 h post-feeding in the PI of fish fed the FO diet. Identical number of bands was observed in zymograms of alkaline proteases of fish fed both diets. No alterations in the histomorphology of PC, AI, PI or DI were noticed in fish fed the VO diets, while in the liver a tendency towards increased hepatocyte vacuolization due to lipid accumulation was observed. Overall, and with the exception of a higher intestine alkaline phosphatase activity, 70% FO replacement by a VO blend in diets for European sea bass resulted in no distinctive alterations on the postprandial pattern of digestive enzyme activities and intestine histomorphology. PMID:26364216

  10. Effect of normal and waxy maize starch on growth, food utilization and hepatic glucose metabolism in European sea bass (Dicentrarchus labrax) juveniles.

    Science.gov (United States)

    Enes, P; Panserat, S; Kaushik, S; Oliva-Teles, A

    2006-01-01

    We determined the effect of dietary starch on growth performance and feed utilization in European sea bass juveniles. Data on the dietary regulation of key hepatic enzymes of the glycolytic, gluconeogenic, lipogenic and amino acid metabolic pathways (hexokinase, HK; glucokinase, GK; pyruvate kinase, PK; fructose-1,6-bisphosphatase, FBPase; glucose-6-phosphatase, G6Pase; glucose-6-phosphate dehydrogenase, G6PD; alanine aminotransferase, ALAT; aspartate aminotransferase, ASAT and glutamate dehydrogenase, GDH) were also measured. Five isonitrogenous (48% crude protein) and isolipidic (14% crude lipids) diets were formulated to contain 10% normal starch (diet NS10), 10% waxy starch (diet WS10), 20% normal starch (diet NS20), 20% waxy starch (diet WS20) or no starch (control diet). Another diet was formulated with no carbohydrate, and contained 68% crude protein and 14% crude lipids (diet HP). Each experimental diet was fed to triplicate groups of 30 fish (initial weight: 23.3 g) on an equivalent feeding scheme for 12 weeks. The best growth performance and feed efficiency were achieved with fish fed the HP diet. Neither the level nor the nature of starch had measurable effects on growth performance of sea bass juveniles. Digestibility of starch was higher with waxy starch and decreased with increasing levels of starch in the diet. Whole-body composition and plasma metabolites, mainly glycemia, were not affected by the level and nature of the dietary starch. Data on enzyme activities suggest that dietary carbohydrates significantly improve protein utilization associated with increased glycolytic enzyme activities (GK and PK), as well as decreased gluconeogenic (FBPase) and amino acid catabolic (GDH) enzyme activities. The nature of dietary carbohydrates tested had little influence on performance criteria.

  11. Antioxidant, antimicrobial and sensorial effects of Rosemary (Rosmarinus officinalis L. and Sage (Salvia officinalis L. on Sea bass (Dicentrarchus labrax L. fillets

    Directory of Open Access Journals (Sweden)

    Can Altınelataman

    2015-09-01

    Full Text Available In this study, effects of sum of vapor distillate and water phase extract of rosemary and sage leaves on sea bass fillets quality, were investigated. For this purpose, effective solution was obtained with same amounts of these plants, cooled down to room temperature, applied to fillets and samples were stored in refrigerated conditions during 13 days. In monitoring period, pH, TVB-N (Total Viable Base –Nitrogen, TBA (Tiobarbutiric acid , color, textural, microbiological and sensorial analysis of samples were done. In rosemary group  Control sample was reached the consumption  limits with the value of 36.35± 0.89 (mg TVB-N /100 g in the 10th day of storage. But with the effect of rosemary extraction, rosemary group 3 more days to reach the spoilage limit. In results, sage was determined as more effective for TBA, TVB-N, rosemary for textural, sensorial and color analysis. For antimicrobial influence, both leaves have positive effectiveness. Especially control goups of both groups reached the spoilage limit in the 6th day of storage period where as the extraction groups were still under the consumptional limits. No spoilage were observed in rancidity values in both groups of the trial.

  12. Teneur en ADN de la larve de Dicentrarchus labrax : évolution ontogénétique et effet de la privation de nourriture

    OpenAIRE

    Bergeron, Jean-pierre; Person, Jeanine

    1997-01-01

    Sea bass Dicentrarchus labrax larvae were hatched and reared for 34 days in the laboratory to test a recent biochemical index (DNA/C) of larval fish nutritional condition. Variations in DNA/C ratio values were monitored during the whole larval ontogenesis of fed individuals and the effect of starvation was assessed at three developmental stages. Three main periods in the relative DNA content variations were determined in close connection with growth patterns during larval ontogenesis.

  13. Biochemical and nutritional traits of sea bass (Dicentrachus labrax from different rearing systems.

    Directory of Open Access Journals (Sweden)

    Andrea Santulli

    2010-01-01

    Full Text Available Farmed European sea bass (Dicentrarchus labrax, sampled from three different culture systems (intensive in sea-cages, intensive in land-based basins and extensive in lagoon and storage basins of salt-work, of the Northern, Central and Southern Italy, were analyzed with the aim to employ nutritional trait to describe and to distinguish the “origin” of the product. Lipid and fatty acid profile, strongly affected by the feeding history and environmental factors, responsible of the nu- tritional and perceived quality of fish product, are proposed as marker of origin.

  14. Hepcidin-Dependent Regulation of Erythropoiesis during Anemia in a Teleost Fish, Dicentrarchus labrax

    Science.gov (United States)

    Caldas, Carolina; Ramos, Miguel F.; Rodrigues, Pedro N. S.

    2016-01-01

    Anemia is a common disorder, characterized by abnormally low levels of red blood cells or hemoglobin. The mechanisms of anemia development and response have been thoroughly studied in mammals, but little is known in other vertebrates, particularly teleost fish. In this study, different degrees of anemia were induced in healthy European sea bass specimens (Dicentrarchus labrax) and at pre-determined time points hematological parameters, liver iron content and the expression of genes involved in iron homeostasis and hematopoiesis, with particular attention on hepcidins, were evaluated. The experimental anemia prompted a decrease in hamp1 expression in all tested organs, in accordance to an increased need for iron absorption and mobilization, with slight increases in hamp2 in the kidney and intestine. The liver was clearly the major organ involved in iron homeostasis, decreasing its iron content and showing a gene expression profile consistent with an increased iron release and mobilization. Although both the spleen and head kidney are involved in erythropoiesis, the spleen was found to assume a more preponderant role in the recovery of erythrocyte levels. The intestine was also involved in the response to anemia, through the increase of iron transporting genes. Administration of Hamp1 or Hamp2 mature peptides showed that only Hamp1 affects hematological parameters and liver iron content. In conclusion, the molecular mechanisms of response to anemia present in sea bass are similar to the ones described for mammals, with these results indicating that the two hepcidin types from teleosts assume different roles during anemia. PMID:27100629

  15. Amyloodinum ocellatum in Dicentrarchus labrax: Study of infection in salt water and freshwater aquaponics.

    Science.gov (United States)

    Nozzi, Valentina; Strofaldi, Silvia; Piquer, Isabel Forner; Di Crescenzo, Davide; Olivotto, Ike; Carnevali, Oliana

    2016-10-01

    This investigation is aimed to improve the knowledge on the physiological alterations occurring at morphological and molecular level in European sea bass naturally infected by A. ocellatum and reared at different salinities. European sea bass juveniles (Dicentrarchus labrax) weighing 20 ± 0.5 g were divided in three aquaponics systems: CTRL, reared at 20 ppt salinity; AFI, reared in freshwater (0 ppt) and infected with the dinoflagellate Amyloodinium ocellatum; ASI, reared at 20 ppt salinity and infected with A. ocellatum. Beta vulgaris plants were introduced in each of the aquaponic systems. Temperature was increased 1 °C every second day from 18 to 25 °C during the experiment. At the end of the trial, liver, brain, intestine and gills were sampled for molecular and histological analyses. A. ocellatum affected D. labrax growth (insulin-like growth factor I, IGF-I) and appetite (Neuropeptide Y, NPY) signals in ASI. Immune system was activated in ASI by the presence of parasites by producing higher levels of Interleukin-1 (IL-1) and Tumor Necrosis Factor α (TNFα). Peroxisome proliferator-activated receptor α (PPAR α), codifying for a protein involved in lipid metabolism, was upregulated in ASI because of the necessity to produce energy to maintain homeostasis. On the contrary, A. ocellatum did not cause signs of infection in AFI as confirmed by gene expression and histological analysis, that were similar to CTRL. However, in freshwater reared fish, a modification of lipid metabolism was observed through a reduction in PPARα gene expression and hepatic lipid content.

  16. Amyloodinum ocellatum in Dicentrarchus labrax: Study of infection in salt water and freshwater aquaponics.

    Science.gov (United States)

    Nozzi, Valentina; Strofaldi, Silvia; Piquer, Isabel Forner; Di Crescenzo, Davide; Olivotto, Ike; Carnevali, Oliana

    2016-10-01

    This investigation is aimed to improve the knowledge on the physiological alterations occurring at morphological and molecular level in European sea bass naturally infected by A. ocellatum and reared at different salinities. European sea bass juveniles (Dicentrarchus labrax) weighing 20 ± 0.5 g were divided in three aquaponics systems: CTRL, reared at 20 ppt salinity; AFI, reared in freshwater (0 ppt) and infected with the dinoflagellate Amyloodinium ocellatum; ASI, reared at 20 ppt salinity and infected with A. ocellatum. Beta vulgaris plants were introduced in each of the aquaponic systems. Temperature was increased 1 °C every second day from 18 to 25 °C during the experiment. At the end of the trial, liver, brain, intestine and gills were sampled for molecular and histological analyses. A. ocellatum affected D. labrax growth (insulin-like growth factor I, IGF-I) and appetite (Neuropeptide Y, NPY) signals in ASI. Immune system was activated in ASI by the presence of parasites by producing higher levels of Interleukin-1 (IL-1) and Tumor Necrosis Factor α (TNFα). Peroxisome proliferator-activated receptor α (PPAR α), codifying for a protein involved in lipid metabolism, was upregulated in ASI because of the necessity to produce energy to maintain homeostasis. On the contrary, A. ocellatum did not cause signs of infection in AFI as confirmed by gene expression and histological analysis, that were similar to CTRL. However, in freshwater reared fish, a modification of lipid metabolism was observed through a reduction in PPARα gene expression and hepatic lipid content. PMID:27542617

  17. Impact of date palm fruits extracts and probiotic enriched diet on antioxidant status, innate immune response and immune-related gene expression of European seabass (Dicentrarchus labrax).

    Science.gov (United States)

    Guardiola, F A; Porcino, C; Cerezuela, R; Cuesta, A; Faggio, C; Esteban, M A

    2016-05-01

    The application of additives in the diet as plants or extracts of plants as natural and innocuous compounds has potential in aquaculture as an alternative to antibiotics and immunoprophylactics. The aim of the current study was to evaluate the potential effects of dietary supplementation of date palm fruit extracts alone or in combination with Pdp11 probiotic on serum antioxidant status, on the humoral and cellular innate immune status, as well as, on the expression levels of some immune-related genes in head-kidney and gut of European sea bass (Dicentrarchus labrax) after 2 and 4 weeks of administration. This study showed for the first time in European sea bass an immunostimulation in several of the parameters evaluated in fish fed with date palm fruits extracts enriched diet or fed with this substance in combination with Pdp 11 probiotic, mainly after 4 weeks of treatment. In the same way, dietary supplementation of mixture diet has positive effects on the expression levels of immune-related genes, chiefly in head-kidney of Dicentrarchus labrax. Therefore, the combination of both could be considered of great interest as potential additives for farmed fish. PMID:27033470

  18. The forebrain-midbrain acts as functional endocrine signaling pathway of Kiss2/Gnrh1 system controlling the gonadotroph activity in the teleost fish European sea bass (Dicentrarchus labrax).

    Science.gov (United States)

    Espigares, Felipe; Carrillo, Manuel; Gómez, Ana; Zanuy, Silvia

    2015-03-01

    Some teleost species, including European sea bass, harbor two different kisspeptin coding genes: kiss1 and kiss2. Both genes are expressed in the brain, but their differential roles in the central control of fish reproduction are only beginning to be elucidated. In this study, we have examined the effects of intracerebroventricular injections of the highly active sea bass peptides Kiss1-15 and Kiss2-12 on spermiating male sea bass. Physiological saline, Kiss1-15, or Kiss2-12 was injected into the third ventricle. To establish the gene expression cascade involved in the action of kisspeptins, the expression of the two sea bass kisspeptin receptor genes (kiss1r and kiss2r) and the three sea bass Gnrh genes (gnrh1, gnrh2, and gnrh3) were analyzed in the forebrain-midbrain and the hypothalamus. In addition, the protein levels of hypothalamic and pituitary Gnrh1 were measured. Blood samples were collected at different times after injection to analyze the effects of kisspeptins on the release of gonadotropins (Lh and Fsh) and androgens (testosterone and 11-ketotestosterone). The present results provide the first evidence that the effects of Kiss2 on central regulation of reproductive function involve the neuroendocrine areas of the forebrain-midbrain in teleost fish. The marked effect of Kiss2 on kiss2r and gnrh1 expression in the forebrain-midbrain and on Gnrh1 release suggest that this neuronal system is involved in the neuroendocrine regulation of gonadotroph activity. This hypothesis was confirmed by a surge of plasma Lh in response to Kiss2, which presumably has a strong stimulatory effect on testosterone release, and thus on sperm quality parameters.

  19. Evaluation of Dicentrarchus labrax Meats and the Vegetable Quality of Beta vulgaris var. cicla Farmed in Freshwater and Saltwater Aquaponic Systems

    Directory of Open Access Journals (Sweden)

    Valentina Nozzi

    2016-09-01

    Full Text Available The purpose of this study is to exploit the euryhaline nature of commercially attractive species for their cultivation in freshwater aquaponic systems. This approach may increase the profitability of aquaponic production in coastal countries where the consumption of marine fish is traditional and of commercial relevance. For this purpose, juvenile European sea bass (Dicentrarchus labrax were reared in an aquaponic freshwater (AFW system and an aquaponic saltwater (ASW system (salinity 20 ppt, in combination with chard (Beta vulgaris var. cicla seedlings, a salt tolerant plant. At the end of the trial, nitrate and phosphate concentration in water significantly increased in the ASW system, suggesting that the ability of B. vulgaris to absorb these substances was limited by salinity. Total reflection X-ray fluorescence spectrometry revealed that the concentration of some oligoelements such as Fe remained lower with respect to the concentration in the freshwater hydroponic solution, in both AFW and ASW. FTIR-Fourier transform infrared spectroscopy on plants showed that growth at high salinity affected their lipid content. In the case of fish, freshwater had no effects on mono- and poly-unsaturated fatty acid profiles, although saturated fatty acids were significantly decreased in D. labrax reared in AFW. Our results demonstrates that it is possible to increase aquaponic profitability by farming D. labrax juveniles in an aquaponic freshwater system together with Beta vulgaris, obtaining good quality products.

  20. Modification Of Photoperiod In Reproduction Of European Seabass (Dicentrarchus Labrax).

    OpenAIRE

    Edmond Hala

    2012-01-01

    The modification of photoperiod is routinely used in aquaculture with the aim to enhance production by manipulating the timing of reproduction in several important fish species. In European sea bass was proved that photoperiod manipulation plays an important role in different aspects of reproduction performance. The present study review the effects of photoperiod modification on some physiological aspects related to period of spawning, sexual differentiation and early puberty of the sea bass ...

  1. The Highly conserved gonadotropin-releasing hormone-2 form acts as a melatonin-releasing factor in the pineal of a teleost fish, the european sea bass Dicentrarchus labrax.

    Science.gov (United States)

    Servili, Arianna; Lethimonier, Christèle; Lareyre, Jean-Jacques; López-Olmeda, José Fernando; Sánchez-Vázquez, Francisco Javier; Kah, Olivier; Muñoz-Cueto, José Antonio

    2010-05-01

    With the exception of modern mammals, most vertebrate species possess two GnRH genes, GnRH-1 and GnRH-2. In addition, in many teleost fish, there is a third gene called GnRH-3. If the main function of GnRH-1 is unambiguously to stimulate gonadotropin release, the other two GnRH forms still lack clear functions. This is particularly true for the highly conserved GnRH-2 that encodes chicken GnRH-II. This GnRH variant is consistently expressed in neurons of the dorsal synencephalon in most vertebrate groups but still has no clear functions supported by anatomical, pharmacological, and physiological data. In this study performed on a perciform fish, the European sea bass, we show for the first time that the pineal organ receives GnRH-2-immunoreactive fibers originating from the synencephalic GnRH-2 neurons. This was shown through a combination of retrograde tracing and immunohistochemistry, using highly specific antibodies. Supporting the presence of GnRH-2 functional targets, RT-PCR data together with the in situ hybridization studies showed that the sea bass pineal gland strongly expressed a GnRH receptor (dlGnRHR-II-2b) with clear selectivity for GnRH-2 and, to a lesser extent, the dlGnRHR-II-1a subtype. Finally, in vitro and in vivo experiments demonstrate stimulatory effects of GnRH-2 on nocturnal melatonin secretion by the sea bass pineal organ. Altogether, these data provide, for the first time in a vertebrate species, converging evidence supporting a role of GnRH-2 in the modulation of fish pineal functions. PMID:20215565

  2. Modification Of Photoperiod In Reproduction Of European Seabass (Dicentrarchus Labrax.

    Directory of Open Access Journals (Sweden)

    Edmond Hala

    2012-09-01

    Full Text Available The modification of photoperiod is routinely used in aquaculture with the aim to enhance production by manipulating the timing of reproduction in several important fish species. In European sea bass was proved that photoperiod manipulation plays an important role in different aspects of reproduction performance. The present study review the effects of photoperiod modification on some physiological aspects related to period of spawning, sexual differentiation and early puberty of the sea bass kept in culture conditions. Long photoperiod followed by short ones and applied before the summer solstice, produce advancements of reproductive period, but . Long photoperiod followed by short ones and applied after the summer solstice, produce delays of reproductive period. The modification of photoperiod offers the possibility of controlling the sexual differentiation of sea bass, but is necessary more work to unveil many of its mechanisms of action. The sea bass in culture conditions face the problem of early maturing males or precocious males. Applying the modified photoperiod in a specific time of early gonadal development can reduce the number of the early maturing males of sea bass.

  3. Variations in toxic metal levels of two fish species, Pomatomus saltatrix and Dicentrarchus labrax, and risk estimation for children.

    Science.gov (United States)

    Yaman, Mehmet; Yaman, Ibrahim H

    2014-02-01

    The concentrations of five toxic metals were monthly determined in two fish species, obtained from fish markets in Turkey during 2010-2011. For the determinations, AAS and ICP-AES were used. The obtained lead concentrations for all studied Pomatomus saltatrix (mean 635 microg x kg(-1)) and Dicentrarchus labrax (mean 463 microg x kg(-1)) samples were found to be significantly higher than the maximum allowances concentration (MAC) of 300 microg x kg(-1). Mean chromium (324 microg x kg(-1)) and Cu (940 microg x kg(-1)) concentrations in Pomatomus saltatrix were higher than in Dicentrarchus labrax (268 microg Cr x kg(-1) and 600 microg Cu x kg(-1)) while Ni in Pomatomus saltatrix (216 microg x kg(-1)) was lower in Dicentrarchus labrax (291 microg x kg(-1)). The estimated non-carcinogenic and carcinogenic health risks by the Target Hazard Quotient and target carcinogenic risk indicate that there are no sytemic effects, and the risk of developing cancer over a human lifetime is between 2-9 in 1000000.

  4. Variations in Toxic Metal Levels of Two Fish Species, Pomatomus saltatrix and Dicentrarchus labrax, and Risk Estimation for Children

    Institute of Scientific and Technical Information of China (English)

    Mehmet Yaman; Ibrahim H. Yaman

    2014-01-01

    The concentrations of five toxic metals were monthly determined in two fish species ,obtained from fish markets in Turkey during 2010-2011 .For the determinations ,AAS and ICP-AES were used .The ob-tained lead concentrations for all studied Pomatomus saltatrix (mean 635 μg · kg -1 ) and Dicentrarchus labrax (mean 463 μg · kg -1 ) samples were found to be significantly higher than the maximum allowances concentra-tion (MAC) of 300 μg · kg -1 .Mean chromium (324 μg · kg -1 ) and Cu (940 μg · kg -1 ) concentrations in Pomatomus saltatrix were higher than in Dicentrarchus labrax (268 μg Cr · kg -1 and 600 μg Cu · kg -1 ) while Ni in Pomatomus saltatrix (216μg · kg -1 ) was lower in Dicentrarchus labrax (291μg · kg -1 ) .The estimated non-carcinogenic and carcinogenic health risks by the Target Hazard Quotient and target carcinogenic risk indi-cate that there are no sytemic effects ,and the risk of developing cancer over a human lifetime is between 2~9 in 1 000 000 .

  5. Predictive model of food intake of the Seabass Dicentrarchus labrax in closed aquaculture systems

    Directory of Open Access Journals (Sweden)

    Allan T Souza

    2015-12-01

    Full Text Available Fish feeding is the main source of waste in aquaculture and is also responsible for most of the environmental impacts derived from this activity. The European Seabass Dicentrarchus labrax is one of the most farmed fishes in the continent, generating an important load of waste on the environment. The consumption of food in fishes is highly dependent on the water temperature and salinity, which create a source of variability on the amount of the ration consumed by fishes. This issue is often neglected by farmers, which aggravates the ration waste. The use of models in aquaculture is increasing in the recent years, and is an excellent tool to combine an increased production with the mitigation of environmental impacts. In this context, this study aimed at developing a predictive model of the D. labrax consumption rate in closed aquaculture systems. Based on the literature review we were able to develop a conceptual diagram and a prototype of the model using the STELLA software (isee®. The preliminary results indicated that the model was capable of predicting the ration consumption of the D. labrax according to the oscillations in the water temperature and salinity. The consumption rate and growth were higher at 21ºC and with a salinity of 28 psu. Laboratory assays are need to validate the models outputs, and they are scheduled to start in October 2015. Finally, the model’s structure, equations, inputs and outputs were integrated into an easy-to-use software developed by the FoodInTech® company, that will help farmers to increase their yields and reduce the waste of ration and the nutrient load on the environment.

  6. High hydrostatic pressure influences the in vitro response to xenobiotics in Dicentrarchus labrax liver.

    Science.gov (United States)

    Lemaire, Benjamin; Mignolet, Eric; Debier, Cathy; Calderon, Pedro Buc; Thomé, Jean Pierre; Rees, Jean François

    2016-04-01

    Hydrostatic pressure (HP) increases by about 1 atmosphere (0.1MPa) for each ten-meter depth increase in the water column. This thermodynamical parameter could well influence the response to and effects of xenobiotics in the deep-sea biota, but this possibility remains largely overlooked. To grasp the extent of HP adaptation in deep-sea fish, comparative studies with living cells of surface species exposed to chemicals at high HP are required. We initially conducted experiments with precision-cut liver slices of a deep-sea fish (Coryphaenoides rupestris), co-exposed for 15h to the aryl hydrocarbon receptor (AhR) agonist 3-methylcholanthrene at HP levels representative of the surface (0.1MPa) and deep-sea (5-15MPa; i.e., 500-1500m depth) environments. The transcript levels of a suite of stress-responsive genes, such as the AhR battery CYP1A, were subsequently measured (Lemaire et al., 2012; Environ. Sci. Technol. 46, 10310-10316). Strikingly, the AhR agonist-mediated increase of CYP1A mRNA content was pressure-dependently reduced in C. rupestris. Here, the same co-exposure scenario was applied for 6 or 15h to liver slices of a surface fish, Dicentrarchus labrax, a coastal species presumably not adapted to high HP. Precision-cut liver slices of D. labrax were also used in 1h co-exposure studies with the pro-oxidant tert-butylhydroperoxide (tBHP) as to investigate the pressure-dependence of the oxidative stress response (i.e., reactive oxygen production, glutathione and lipid peroxidation status). Liver cells remained viable in all experiments (adenosine triphosphate content). High HP precluded the AhR agonist-mediated increase of CYP1A mRNA expression in D. labrax, as well as that of glutathione peroxidase, and significantly reduced that of heat shock protein 70. High HP (1h) also tended per se to increase the level of oxidative stress in liver cells of the surface fish. Trends to an increased resistance to tBHP were also noted. Whether the latter observation truly

  7. Multi-element otolith chemistry of juvenile sole ( Solea solea), whiting ( Merlangius merlangus) and European seabass ( Dicentrarchus labrax) in the Thames Estuary and adjacent coastal regions

    Science.gov (United States)

    Leakey, Chris D. B.; Attrill, Martin J.; Fitzsimons, Mark F.

    2009-04-01

    Estuaries are regarded as valuable nursery habitats for many commercially important marine fishes, potentially providing a thermal resource, refuge from predators and a source of abundant prey. To assess the extent of estuarine use by juvenile (0+) common sole ( Solea solea), whiting ( Merlangius merlangus) and European seabass ( Dicentrarchus labrax) we: (1) developed techniques to distinguish between estuarine and coastally-caught juveniles using otolith chemistry; and (2) examined the accuracy with which multi-elemental signatures could re-classify juveniles to their region of collection. High-resolution solution-based inductively coupled plasma mass spectrometry (HB-SB-ICPMS) was used to quantify 32 elements within the juvenile otoliths; 14 elements occurred above detection limits for all samples. Some elemental distributions demonstrated clear differences between estuarine and coastally-caught fish. Multivariate analysis of the otolith chemistry data resulted in 95-100% re-classification accuracy to the region of collection. Estuarine and coastal signatures were most clearly defined for sole which, compared to bass and whiting, have low mobility and are less likely to move from estuarine to coastal habitats between larval settlement and later migration to adult stocks. Sole were the only species to reveal an energetic benefit associated with an estuarine juvenile phase. The physiological ability of bass to access upper estuarine regions was consistent with some elemental data, while the high mobility and restricted range of whiting resulted in less distinct otolith chemistries.

  8. Phylogenetic analysis of antimicrobial lactic acid bacteria from farmed seabass Dicentrarchus labrax.

    Science.gov (United States)

    Bourouni, Ouissal Chahad; El Bour, Monia; Calo-Mata, Pilar; Mraouna, Radhia; Abedellatif, Boudabous; Barros-Velàzquez, Jorge

    2012-04-01

    The use of lactic acid bacteria (LAB) in the prevention or reduction of fish diseases is receiving increasing attention. In the present study, 47 LAB strains were isolated from farmed seabass ( Dicentrarchus labrax ) and were phenotypically and phylogenetically analysed by 16S rDNA and randomly amplified polymorphic DNA - polymerase chain reaction (RAPD-PCR). Their antimicrobial effect was tested in vitro against a wide variety of pathogenic and spoilage bacteria. Most of the strains isolated were enterococci belonging to the following species: Enterococcus faecium (59%), Enterococcus faecalis (21%), Enterococcus sanguinicola (4 strains), Enterococcus mundtii (1 strain), Enterococcus pseudoavium (1 strain), and Lactococcus lactis (1 strain). An Aerococcus viridans strain was also isolated. The survey of their antimicrobial susceptibility showed that all isolates were sensitive to vancomycin and exhibited resistance to between 4 and 10 other antibiotics relevant for therapy in human and animal medicine. Different patterns of resistance were noted for skin and intestines isolates. More than 69% (32 strains) of the isolates inhibited the growth of the majority of pathogenic and spoilage bacteria tested, including Listeria monocytogenes, Staphylococcus aureus, Aeromonas hydrophila, Aeromonas salmonicida, Vibrio anguillarum, and Carnobacterium sp. To our knowledge, this is the first report of bioactive enterococcal species isolated from seabass that could potentially inhibit the undesirable bacteria found in food systems.

  9. Phylogenetic analysis of antimicrobial lactic acid bacteria from farmed seabass Dicentrarchus labrax.

    Science.gov (United States)

    Bourouni, Ouissal Chahad; El Bour, Monia; Calo-Mata, Pilar; Mraouna, Radhia; Abedellatif, Boudabous; Barros-Velàzquez, Jorge

    2012-04-01

    The use of lactic acid bacteria (LAB) in the prevention or reduction of fish diseases is receiving increasing attention. In the present study, 47 LAB strains were isolated from farmed seabass ( Dicentrarchus labrax ) and were phenotypically and phylogenetically analysed by 16S rDNA and randomly amplified polymorphic DNA - polymerase chain reaction (RAPD-PCR). Their antimicrobial effect was tested in vitro against a wide variety of pathogenic and spoilage bacteria. Most of the strains isolated were enterococci belonging to the following species: Enterococcus faecium (59%), Enterococcus faecalis (21%), Enterococcus sanguinicola (4 strains), Enterococcus mundtii (1 strain), Enterococcus pseudoavium (1 strain), and Lactococcus lactis (1 strain). An Aerococcus viridans strain was also isolated. The survey of their antimicrobial susceptibility showed that all isolates were sensitive to vancomycin and exhibited resistance to between 4 and 10 other antibiotics relevant for therapy in human and animal medicine. Different patterns of resistance were noted for skin and intestines isolates. More than 69% (32 strains) of the isolates inhibited the growth of the majority of pathogenic and spoilage bacteria tested, including Listeria monocytogenes, Staphylococcus aureus, Aeromonas hydrophila, Aeromonas salmonicida, Vibrio anguillarum, and Carnobacterium sp. To our knowledge, this is the first report of bioactive enterococcal species isolated from seabass that could potentially inhibit the undesirable bacteria found in food systems. PMID:22439634

  10. Effects of short term feeding of some marine microalgae on the microbial profile associated with Dicentrarchus labrax post larvae

    Directory of Open Access Journals (Sweden)

    Heba S. El-Sayed

    2014-01-01

    Full Text Available This study investigates the microbial profile and antimicrobial activity of four marine microalgae species, Tetraselmis chuii, Nannochloropsis salina, Isochrysis galbana and Chlorella salina used in aquaculture of Dicentrarchus labrax in the post larval stage to estimate which was the best algal species that could be used as a green water technique and achieving the maximum rate of growth and survival of D. labrax post larvae. The results represented a significant increase in the length and width of D. labrax at p < 0.05 recorded in the case of enrichment with I. galbana followed by N. salina, and the most weight was recorded in the case of N. salina as compared with the control. Significant increase in percentage of survival of D. labrax was recorded in the case of C. salina and T. chuii (70% and 60.1%, respectively as compared with the control (22%. The antibacterial activity (AU of the different microalgal ethanolic extracts against fish indicator pathogens was determined. The results indicated that the ethanolic extracts of C. salina and T. chuii have the most positive records against the fish indicator pathogens (Escherichia coli, Pseudomonas aeruginosa, Vibrio damsela, Vibrio fluvialis and Aeromonas hydrophila. The current study was extended to determine the GC–MS of ethanolic extract of C. salina and T. chuii. The main constituents detected in the ethanolic extract were organic acids like hexadecanoic acid, octadecanoic acid, and an acyclic diterpene alcohol like phytol.

  11. Early treatment with Lactobacillus delbrueckii strain induces an increase in intestinal T-cells and granulocytes and modulates immune-related genes of larval Dicentrarchus labrax (L.).

    Science.gov (United States)

    Picchietti, Simona; Fausto, Anna Maria; Randelli, Elisa; Carnevali, Oliana; Taddei, Anna Rita; Buonocore, Francesco; Scapigliati, Giuseppe; Abelli, Luigi

    2009-03-01

    Lactobacillus delbrueckii ssp. delbrueckii (AS13B), isolated from the gut of adult Dicentrarchus labrax, was administered live to developing sea bass using rotifers and Artemia as live carriers. Immune-related gene transcripts were quantified in post-larvae at day 70 post-hatch (ph) and histology, electron microscopy and immunocytochemistry of the intestinal tissue were performed at day 74 ph. Since the probiotic was orally administered the studies were focused on intestinal immunity. In treated fish gut integrity was unaffected, while the density of T-cells and acidophilic granulocytes in the intestinal mucosa was significantly higher than in controls. Probiotic-induced increases in intestinal T-cells and total body TcR-beta transcripts are first reported in fish. Significantly lower IL-1beta transcripts and a trend towards lower IL-10, Cox-2 and TGF-beta transcription were found in the treated group. Evidence is provided that early feeding with probiotic-supplemented diet stimulated the larval gut immune system and lowered transcription of key pro-inflammatory genes.

  12. Oxidative stress, genotoxicity and histopathology biomarker responses in Mugil cephalus and Dicentrarchus labrax gill exposed to persistent pollutants. A field study in the Bizerte Lagoon: Tunisia.

    Science.gov (United States)

    Ameur, Walid Ben; El Megdiche, Yassine; de Lapuente, Joaquin; Barhoumi, Badreddine; Trabelsi, Souad; Ennaceur, Soukaina; Camps, Lydia; Serret, Joan; Ramos-López, David; Gonzalez-Linares, Javier; Touil, Soufiane; Driss, Mohamed Ridha; Borràs, Miquel

    2015-09-01

    The use of biomarkers has become an important tool for modern environmental assessment as they can help to predict pollutants involved in the monitoring program. Despite the importance of fish gill in several functions (gaseous exchange, osmotic and ionic regulation, acid-base balance and nitrogenous waste) its use in coastal water biomonitoring focusing on protection and damage is scarce. This field study investigates biochemical (catalase, superoxide dismutase, lipid peroxidation), molecular (DNA integrity) and morphological (histology) parameters in gill of mullet (Mugil cephalus) and sea bass (Dicentrarchus labrax) and originating from Bizerte lagoon (a coastal lagoon impacted by different anthropogenic activities) and from the Mediterranean Sea (a reference site). Remarkable alterations in the activities of oxidative stress enzymes and DNA integrity in the tissue of the two studied fish species were detected in Bizerte Lagoon. The study of histopathological alterations of gills in both two fish species from Bizerte Lagoon suggest thickening of primary lamellae, cellular hyperplasia, aneurism, curving, shortening and fusion of secondary lamellae. The adopted approach, considering simultaneously protection responses and damaging effects, revealed its usefulness on the pollution assessment. PMID:25912422

  13. Dynamics of gene expression patterns during early development of the European seabass (Dicentrarchus labrax).

    Science.gov (United States)

    Kaitetzidou, E; Xiang, J; Antonopoulou, E; Tsigenopoulos, C S; Sarropoulou, E

    2015-05-01

    Larval and embryonic stages are the most critical period in the life cycle of marine fish. Key developmental events occur early in development and are influenced by external parameters like stress, temperature, salinity, and photoperiodism. Any failure may cause malformations, developmental delays, poor growth, and massive mortalities. Advanced understanding of molecular processes underlying marine larval development may lead to superior larval rearing conditions. Today, the new sequencing and bioinformatic methods allow transcriptome screens comprising messenger (mRNA) and microRNA (miRNA) with the scope of detecting differential expression for any species of interest. In the present study, we applied Illumina technology to investigate the transcriptome of early developmental stages of the European seabass (Dicentrarchus labrax). The European seabass, in its natural environment, is a euryhaline species and has shown high adaptation processes in early life phases. During its embryonic and larval phases the European seabass lives in a marine environment and as a juvenile it migrates to coastal zones, estuaries, and lagoons. Investigating the dynamics of gene expression in its early development may shed light on factors promoting phenotypic plasticity and may also contribute to the improvement and advancement of rearing methods of the European seabass, a species of high economic importance in European and Mediterranean aquaculture. We present the identification, characterization, and expression of mRNA and miRNA, comprising paralogous genes and differentially spliced transcripts from early developmental stages of the European seabass. We further investigated the detection of possible interactions of miRNA with mRNA.

  14. Bioaccumulation and elimination of mercury in juvenile seabass (Dicentrarchus labrax) in a warmer environment.

    Science.gov (United States)

    Maulvault, Ana Luísa; Custódio, Ana; Anacleto, Patrícia; Repolho, Tiago; Pousão, Pedro; Nunes, Maria Leonor; Diniz, Mário; Rosa, Rui; Marques, António

    2016-08-01

    Warming is an expected impact of climate change that will affect coastal areas in the future. These areas are also subjected to strong anthropogenic pressures leading to chemical contamination. Yet, the consequences of both factors for marine ecosystems, biota and consumers are still unknown. The present work aims to investigate, for the first time, the effect of temperature increase on bioaccumulation and elimination of mercury [(total mercury (THg) and methylmercury (MeHg)] in three tissues (muscle, liver, and brain) of a commercially important seafood species - European seabass (Dicentrarchus labrax). Fish were exposed to the ambient temperature currently used in seabass rearing (18°C) and to the expected ocean warming (+4°C, i.e. 22°C), as well as dietary MeHg during 28 days, followed by a depuration period of 28 days fed with a control diet. In both temperature exposures, higher MeHg contents were observed in the brain, followed by the muscle and liver. Liver registered the highest elimination percentages (EF; up to 64% in the liver, 20% in the brain, and 3% in the muscle). Overall, the results clearly indicate that a warming environment promotes MeHg bioaccumulation in all tissues (e.g. highest levels in brain: 8.1mgkg(-1) ww at 22°C against 6.2mgkg(-1) ww at 18°C after 28 days of MeHg exposure) and hampers MeHg elimination (e.g. liver EF decreases after 28 days of depuration: from 64.2% at 18°C to 50.3% at 22°C). These findings suggest that seafood safety may be compromised in a warming context, particularly for seafood species with contaminant concentrations close to the current regulatory levels. Hence, results point out the need to strengthen research in this area and to revise and/or adapt the current recommendations regarding human exposure to chemical contaminants through seafood consumption, in order to integrate the expected effects of climate change. PMID:27179934

  15. Dietary tryptophan and methionine as modulators of European seabass (Dicentrarchus labrax) immune status and inflammatory response.

    Science.gov (United States)

    Machado, Marina; Azeredo, Rita; Díaz-Rosales, Patricia; Afonso, António; Peres, Helena; Oliva-Teles, Aires; Costas, Benjamín

    2015-02-01

    Amino acids regulate key metabolic pathways important to immune responses and their nutritional supply may increase synthesis of immune-related proteins. The present study aimed to evaluate the effects of dietary supplementation of tryptophan and methionine on European seabass (Dicentrarchus labrax) cellular and humoral status. The immunomodulatory effects of tryptophan and methionine during an inflammatory insult was also evaluated after intraperitoneal injection with inactivated Photobacterium damselae subsp. piscicida (Phdp). A practical isonitrogenous (45% crude protein) and isolipidic (16% crude fat) diets was formulated to include fish meal and a blend of plant feedstuffs as protein sources and fish oil as the main lipid source (CRL diet). Two other diets were formulated similar to the control but including L-tryptophan or L-methionine at ×2 the requirement level (diets TRP and MET, respectively). European seabass weighing 275 g were fed the experimental diets for a period of 15 days before being sampled (trial 1). Then, fish were subjected to a peritoneal inflammation by intraperitoneally injecting UV killed Phdp (10(6) colony forming units ml(-1)) and sampled following 4 and 24 h post-injection (trial 2). Fish injected with a saline solution served as control. The haematological profile, peripheral cell dynamics and several plasma immune parameters were determined in trials 1 and 2, whereas cell migration to the inflammatory focus was also determined in trial 2. MET positively affected European seabass immune status by improving the peripheral leucocyte response, complement activity and bactericidal capacity, a stronger cellular recruitment to the inflammatory focus, and higher plasma peroxidase and bactericidal activities. TRP also seemed to improve immunostimulation, as there was a trend to augment both cell-mediated immunity and humoral capacity. However, TRP failed to improve an inflammatory response, verified by a decrease in blood phagocyte numbers

  16. Expression and distribution of the glucocorticoid receptor DlGR1 in the teleost Dicentrarchus labrax brain

    Directory of Open Access Journals (Sweden)

    Nicolò Parrinello

    2010-01-01

    Full Text Available Cortisol is the main corticosteroid secreted by the interrenal cells of the head kidney and it exerts a role in mantaining the omeostatic status in fish. In teleosts its effects are mediated through intracellular receptors expressed in several tissues, that are ligand-dependent transcription factors by binding to specific tissue DNA sequences. In Dicentrarchus labrax we previously cloned and sequenced a glucocorticoid receptor, DlGR1, isolated from leukocytes of peritoneal cavity. In this work we showed mRNA expression and tissue immunohistochemical localization of brain DlGR1 by in situ hybridization assays, with a riboprobe with DlGR1 cDNA trascriptional activation domain, and by immunohistochemical methods, using a specific antibody for a selected sequence of the receptor tran- scriptional domain. The mRNA and the protein are expressed in pyramidal cells of the optic lobe and in the small globular neurons of the diencephalon.

  17. Coastal versus estuarine nursery grounds: Effect of differential temperature and heat waves on juvenile seabass, Dicentrarchus labrax

    Science.gov (United States)

    Vinagre, Catarina; Narciso, Luís; Cabral, Henrique N.; Costa, Maria J.; Rosa, Rui

    2012-08-01

    This study investigates the biological responses of juvenile fish (Dicentrarchus labrax), that live in both coastal and estuarine nurseries, to differential temperatures and summer heat wave events. More specifically, we compared mortality, growth, condition, metabolic response and thermal sensitivity of 0-group juveniles of D. labrax at temperatures that reflect the average summer temperature that they encounter in coastal and estuarine nurseries, and also the temperatures that they endure inside estuaries during heat wave events. The low mortality and peak growth and condition values registered at 24 °C suggest that estuarine average summer temperatures are more beneficial for the juveniles than coastal ones. The estuarine water temperature attained during heat waves resulted in higher mortality, arrested growth, lower condition and a steep increase in metabolism, indicating that this species is probably under thermal stress at 28 °C. Consequently, future predictions of frequent and prolonged heat waves in Southern Europe are expected to induce negative impacts in the biology and metabolic ecology of 0-group seabass juveniles in estuarine nurseries.

  18. Early genotoxic response and accumulation induced by waterborne copper, lead, and arsenic in European seabass, Dicentrarchus labrax.

    Science.gov (United States)

    Canalejo, Antonio; Diaz-de-Alba, Margarita; Granado-Castro, M Dolores; Cordoba, Francisco; Espada-Bellido, Estrella; Galindo-Riaño, M Dolores; Torronteras, Rafael

    2016-02-01

    Cu, Pb, and As, which are among the most abundant metals in the aquatic environment, are also among the most health-threatened by causing diverse cellular injuries. The aim of this study was to assess and compare the potential early induction of genotoxic effects after waterborne Cu, Pb, and As exposure in European seabass, Dicentrarchus labrax, a commercial widely cultured fish, using the micronucleus (MN) assay in peripheral blood erythrocytes. Fish were exposed under laboratory conditions to nominal solutions ranging 0-10 mg/L for 24 and 96 h. Furthermore, actual metal ion concentrations were measured by inductively coupled plasma atomic emission spectroscopy (ICP-AES) or differential pulse anodic stripping voltammetry (DPASV) in water and four fish tissues differentially related to environmental exposition and metal accumulation, i.e. the gills, liver, muscle, and brain. Dose-dependent increases of micronuclei (MNi) frequency were observed after these very short exposures; based on measured metal concentrations in water, the genotoxic effect ordered as Cu > As > Pb. Significant genotoxic effect at 0.009 mg/L Cu, 0.57 mg/L Pb, and 0.01 mg/L As was seen. For Cu and Pb these are only slightly higher, but for As it is notably lower than the USEPA criteria of maximum concentration to prevent acute toxicity in aquatic organisms. Furthermore, genotoxicity was differentially related to metal accumulation. MNi frequency correlated positively with the content of Pb in all the organs, with the content of As in liver and gills and only with the content of Cu in the brain. In conclusion, our findings raised environmental concerns because these depicted a genotoxic potential of Cu, Pb, and As after a very short exposure to low but environmentally relevant concentrations, too close to regulatory thresholds. In addition, the MN test in D. labrax could be considered an early biomarker of genotoxicity induced by these metals in fish.

  19. Importance of prebiotics in aquaculture as immunostimulants. Effects on immune system of Sparus aurata and Dicentrarchus labrax.

    Science.gov (United States)

    Carbone, Donatella; Faggio, Caterina

    2016-07-01

    Infectious diseases in fish represent a major problem for the aquaculture field as they produce extensive damages and loss. Over the last few years, with increased development of the aquaculture industry, different methods have been used to contrast these pathologies. Common interest has led to the use of components (as additives in diets) that could contrast diseases without causing any negative impact on the environment. These components are represented by prebiotics, probiotics, and plant extracts. In this review, the effects of prebiotics are described. Prebiotics are indigestible fibres fermented by gut enzymes and commensal bacteria, whose beneficial effects are due to the by-products generated from fermentation. The influence of pre-biotics on the immune system of fish is called immunosaccharides. Mannanoligosaccharides (MOS), Fructooligosaccharides (FOS) and Inulin act at different levels in the innate immune response. For example, through phagocytosis, lysozyme activity, and the complement system activity, an increase in fish growth and an amelioration of their health status is brought about. In this review, the use of prebiotics in aquaculture, such as immunostimulants, has been highlighted: particularly in two teleost fish species, Sparus aurata and Dicentrarchus labrax. The results demonstrate that the road is still long and further studies are required, but the use of prebiotics, individually or coupled together, can open the doors to pioneering a new model of alternative components to antimicrobial agents. PMID:27074444

  20. Role of dietary seaweed supplementation on growth performance, digestive capacity and immune and stress responsiveness in European seabass (Dicentrarchus labrax

    Directory of Open Access Journals (Sweden)

    Maria João Peixoto

    2016-05-01

    Full Text Available This work aimed to appraise growth performance, digestive capacity and immune and stress responsiveness in European seabass (Dicentrarchus labrax when fed diets supplemented with seaweeds. A control diet (without supplementation was tested against 6 practical diets supplemented either with Gracilaria spp., Ulva spp., or Fucus spp., at 2.5 or 7.5% levels, plus an additional diet with a blend of the three seaweeds, each supplemented at 2.5% (Mix. Seabass juveniles (24.0 ± 6.3 g were fed the experimental diets for 84 days. Dietary seaweed supplementation had no effect on growth rate (DGI: 1.0–1.1, voluntary feed intake (11.3–12.6 g kg −1 ABW−1 day−1, feed conversion ratio (FCR: 1.2–1.4 and protein efficiency ratio (PER: 1.5–1.8. Lipase activity was significantly higher in fish fed Mix diet when compared to control (P < 0.05. Glutathione peroxidase (GPx was significantly affected by seaweeds presence regardless its level of supplementation (P < 0.05. The innate immune system was significantly altered by dietary seaweed supplementation (P < 0.05. Fish fed the 7.5% seaweed supplemented diets had a significant decrease in the ACH50 level, when compared to fish fed 2.5% seaweed supplemented diets (P < 0.05. Moreover, a combined effect of seaweed and supplementation level significantly affected lysozyme (LYS activity (P < 0.05. Fish fed diet with Ulva at 2.5% had an increase in LYS when compared to fish fed control and Ulva at 7.5% diets. Overall, our results indicate that the use of dietary seaweed supplementation improves immune and antioxidant responses in European seabass without compromising growth performance.

  1. Compensatory feeding in the sea bass after fasting and physical stress

    OpenAIRE

    Rubio, V. C.; Sánchez, Elisa; Cerdá-Reverter, José Miguel

    2010-01-01

    This work was designed to study the re-feeding dynamics of sea bass (Dicentrarchus labrax) following food deprivation and food-intake reduction induced by physical stressors. Two experiments were carried out, using one hundred sea bass distributed into 10 tanks provided with self-feeders and kept under natural environmental conditions. The first experiment focused on the self-feeding behaviour after two short-term fasting periods (2 and 9 days). The second experiment examined the effects of t...

  2. Evaluation of garlic and onion powder as phyto-additives in the diet of sea bass (Dicentrarcus labrax)

    OpenAIRE

    Norhan E. Saleh; Fady R. Michael; Mohamed M. Toutou

    2015-01-01

    The present study is a preliminary work which was conducted to evaluate the impact of adding 3 levels of garlic (10, 20 and 30 g/kg diet) as well as onion powder (5, 10 and 20 g/kg diet) in a basal diet of sea bass (Dicentrarcus labrax) fry (initial weight = 0.4 ± 0.05 g/fish) on growth performance, feed utilization, body composition, survival rate and some hematological parameters. The present results suggested that dietary garlic or onion powder administrated separately at levels of 30 or 1...

  3. Cryopreservation of sea bass (Dicentrarchus labrax) spermatozoa in experimental and production simulating conditions

    OpenAIRE

    Fauvel, Christian; Suquet, Marc; Dreanno, Catherine; Zonno, Vincenzo; Menu, Bruno

    1998-01-01

    Un protocole de cryoconservation du sperme mis au point chez le turbot a été appliqué au loup en petit (250 μL) et grand volume (1,5 mL). Dilué dans le milieu de Mounib et congelé par une baisse de température de −65 °C·min−1, le sperme a présenté à la décongélation une motilité initiale comparable à celle du témoin, mais une chute de motilité significativement supérieure (p< 0,001, n= 10) aux temps d'observation ultérieurs. L'insémination de petits volumes (2mL, soient appro...

  4. Heritability of cortisol response to confinement stress in European sea bass dicentrarchus labrax

    NARCIS (Netherlands)

    Volckaert, F.A.M.; Hellemans, B.; Batargias, C.; Louro, B.; Massault, C.; Houdt, Van J.K.J.; Haley, C.; Koning, de D.J.; Canario, A.V.M.

    2012-01-01

    Background: In fish, the most studied production traits in terms of heritability are body weight or growth, stress or disease resistance, while heritability of cortisol levels, widely used as a measure of response to stress, is less studied. In this study, we have estimated heritabilities of two gro

  5. Relationships among traits of aerobic and anaerobic swimming performance in individual European sea bass Dicentrarchus labrax.

    Science.gov (United States)

    Marras, Stefano; Killen, Shaun S; Domenici, Paolo; Claireaux, Guy; McKenzie, David J

    2013-01-01

    Teleost fishes exhibit wide and temporally stable inter-individual variation in a suite of aerobic and anaerobic locomotor traits. One mechanism that could allow such variation to persist within populations is the presence of tradeoffs between aerobic and anaerobic performance, such that individuals with a high capacity for one type of performance have a reduced capacity for the other. We investigated this possibility in European seabass Dicentrarchuslabrax, each measured for a battery of indicators of maximum locomotor performance. Aerobic traits comprised active metabolic rate, aerobic scope for activity, maximum aerobic swimming speed, and stride length, using a constant acceleration test. Anaerobic traits comprised maximum speed during an escape response, maximum sprint speed, and maximum anaerobic burst speed during constant acceleration. The data provided evidence of significant variation in performance among individuals, but there was no evidence of any trade-offs among any traits of aerobic versus anaerobic swimming performance. Furthermore, the anaerobic traits were not correlated significantly among each other, despite relying on the same muscular structures. Thus, the variation observed may reflect trade-offs with other morphological, physiological or behavioural traits.

  6. Relationships among traits of aerobic and anaerobic swimming performance in individual European sea bass Dicentrarchus labrax.

    Directory of Open Access Journals (Sweden)

    Stefano Marras

    Full Text Available Teleost fishes exhibit wide and temporally stable inter-individual variation in a suite of aerobic and anaerobic locomotor traits. One mechanism that could allow such variation to persist within populations is the presence of tradeoffs between aerobic and anaerobic performance, such that individuals with a high capacity for one type of performance have a reduced capacity for the other. We investigated this possibility in European seabass Dicentrarchuslabrax, each measured for a battery of indicators of maximum locomotor performance. Aerobic traits comprised active metabolic rate, aerobic scope for activity, maximum aerobic swimming speed, and stride length, using a constant acceleration test. Anaerobic traits comprised maximum speed during an escape response, maximum sprint speed, and maximum anaerobic burst speed during constant acceleration. The data provided evidence of significant variation in performance among individuals, but there was no evidence of any trade-offs among any traits of aerobic versus anaerobic swimming performance. Furthermore, the anaerobic traits were not correlated significantly among each other, despite relying on the same muscular structures. Thus, the variation observed may reflect trade-offs with other morphological, physiological or behavioural traits.

  7. Vaccination of European sea bass fry through bioencapsulation of Artemia nauplii

    OpenAIRE

    Chair, M.; Gapasin, R.S.J.; Dehasque, M.; Sorgeloos, P.

    1994-01-01

    European sea bass (Dicentrarchus labrax) fry vaccinated orally by bioencapsulation in Artemia nauplii or by bath method exhibited better performance than control fish in terms of growth, food conversion and resistance to stress. The comparable survival between vaccinated and non-vaccinated animals suggests that vaccination methods are stressful. The present study shows that oral vaccination can be used to enhance growth in fish fry.

  8. Gene Expression Profiling Reveals Functional Specialization along the Intestinal Tract of a Carnivorous Teleostean Fish (Dicentrarchus labrax)

    Science.gov (United States)

    Calduch-Giner, Josep A.; Sitjà-Bobadilla, Ariadna; Pérez-Sánchez, Jaume

    2016-01-01

    High-quality sequencing reads from the intestine of European sea bass were assembled, annotated by similarity against protein reference databases and combined with nucleotide sequences from public and private databases. After redundancy filtering, 24,906 non-redundant annotated sequences encoding 15,367 different gene descriptions were obtained. These annotated sequences were used to design a custom, high-density oligo-microarray (8 × 15 K) for the transcriptomic profiling of anterior (AI), middle (MI), and posterior (PI) intestinal segments. Similar molecular signatures were found for AI and MI segments, which were combined in a single group (AI-MI) whereas the PI outstood separately, with more than 1900 differentially expressed genes with a fold-change cutoff of 2. Functional analysis revealed that molecular and cellular functions related to feed digestion and nutrient absorption and transport were over-represented in AI-MI segments. By contrast, the initiation and establishment of immune defense mechanisms became especially relevant in PI, although the microarray expression profiling validated by qPCR indicated that these functional changes are gradual from anterior to posterior intestinal segments. This functional divergence occurred in association with spatial transcriptional changes in nutrient transporters and the mucosal chemosensing system via G protein-coupled receptors. These findings contribute to identify key indicators of gut functions and to compare different fish feeding strategies and immune defense mechanisms acquired along the evolution of teleosts. PMID:27610085

  9. Comparative pathogenicity study of ten different betanodavirus strains in experimentally infected European sea bass, Dicentrarchus labrax (L.)

    DEFF Research Database (Denmark)

    Vendramin, Niccolò; Toffan, A.; Mancin, M.;

    2014-01-01

    Viral encephalopathy and retinopathy (VER), otherwise known as viral nervous necrosis (VNN), is a severe pathological condition caused by RNA viruses belonging to the Nodaviridae family, genus Betanodavirus. The disease, described in more than 50 fish species worldwide, is considered as the most...... serious viral threat affecting marine farmed species in the Mediterranean region, thus representing one of the bottlenecks for further development of the aquaculture industry. To date, four different genotypes have been identified, namely red-spotted grouper nervous necrosis virus (RGNNV), striped jack...... of these genetically different strains could be the reason for the differences in mortality observed in the field. However, very little experimental data are available on the pathogenicity of these viruses in farmed fish. Therefore, in this study, the pathogenicity of 10 isolates has been assessed with an in vivo...

  10. Pea protein concentrate as a substitute for fish meal protein in sea bass diet

    Directory of Open Access Journals (Sweden)

    E. Badini

    2010-01-01

    Full Text Available Pea seeds, even if lower in protein than oilseed meals, have been shown to successfully replace moderate amounts of fish meal protein in diets for carnivorous fish species (Kaushik et al., 1993, Gouveia and Davies, 2000. A further processing of such pulses provides concentrated protein products which look very promising as fish meal substitutes in aquafeeds (Thiessen et al., 2003. The aim of the present study was to evaluate nutrient digestibility, growth response, nutrient and energy retention efficiencies and whole body composition of sea bass (Dicentrarchus labrax, L. fed complete diets in which a pea protein concentrate (PPC was used to replace graded levels of fish meal protein.

  11. Organochlorines in cultivated sea bass and in diet

    Energy Technology Data Exchange (ETDEWEB)

    Gil, O.; Antunes, P. [Inst. Nacional de Investigacao Agraria e das Pescas/IPIMAR, Lisboa (Portugal)

    2004-09-15

    The main source of organochlorines bioaccumulation seems to be the food and marine species are considered one of the most important sources of organochlorines for human population. Therefore, it is essential to inventory the levels of these contaminants in marine organisms and find ways to reduce these levels. Aquaculture has been developed in the past decades and sea bass (Dicentrarchus labrax) is one of the main species produced in Portugal. The levels of organochlorines of commercial formulations of diets used in fish farming can influence the concentrations of fish produced in these systems of culture. In this study eighteen polychlorinated biphenyl (PCB) congeners and p,p'-DDT compounds were quantified in cultivated sea bass from two farms and the influence of size and diet contamination in bioaccumulation of these compounds was evaluated.

  12. Trace elements and metals in farmed sea bass and gilthead bream from Tenerife Island, Spain.

    Science.gov (United States)

    Rubio, C; Jalilli, A; Gutiérrez, A J; González-Weller, D; Hernández, F; Melón, E; Burgos, A; Revert, C; Hardisson, A

    2011-11-01

    The aim of this study was to determine the levels of metals (Ca, K, Na, Mg) and trace metals (Ni, Fe, Cu, Mn, Zn, Pb, Cd) in two fish species (gilthead bream [Sparus aurata] and sea bass [Dicentrarchus labrax]) collected from fish farms located along the coast of Tenerife Island. Ca, K, Na, Mg, Fe, Cu, Zn, and Mn were measured by flame atomic absorption spectrometry, whereas Pb, Cd, and Ni were determined using graphite furnace atomic absorption spectrometry. Mean Fe, Cu, Mn, and Zn contents were 3.09, 0.59, 0.18, and 8.11 mg/kg (wet weight) in S. aurata and 3.20, 0.76, 0.24, and 10.11 mg/kg (wet weight) in D. labrax, respectively. In D. labrax, Ca, K, Na, and Mg levels were 1,955, 2,787, 699.7, and 279.2 mg/kg (wet weight), respectively; in S. aurata, they were 934.7, 3,515, 532.8, and 262.8 mg/kg (wet weight), respectively. The Pb level in S. aurata was 7.28 ± 3.64 μg/kg (wet weight) and, in D. labrax, 4.42 ± 1.56 μg/kg (wet weight). Mean Cd concentrations were 3.33 ± 3.93 and 1.36 ± 1.53 μg/kg (wet weight) for D. labrax and S. aurata, respectively. All Pb and Cd levels measured were well below the accepted European Commission limits, 300 and 50 μg/kg for lead and cadmium, respectively.

  13. Energy savings in sea bass swimming in a school: measurements of tail beat frequency and oxygen consumption at different swimming speeds

    DEFF Research Database (Denmark)

    Herskin, J; Steffensen, JF

    1998-01-01

    Tail beat frequency of sea bass, Dicentrarchus labrax (L.) (23.5 ± 0·5 cm, LT), swimming at the front of a school was significantly higher than when swimming at the rear, for all water velocities tested from 14·8 to 32 cm s-1. The logarithm of oxygen consumption rate, and the tail beat frequency...... of solitary swimming sea bass (28·8 ± 0·4 cm, LT), were each correlated linearly with swimming speed, and also with one another. The tail beat frequency of individual fish was 9-14% lower when at the rear of a school than when at the front, corresponding to a 9-23% reduction in oxygen consumption rate....

  14. Accumulation of 137Cs in the European Sea Bass Dicentrarchus Labrax (L.) in a salinity gradient: Importance of uptake via gills, diet and ingested water

    OpenAIRE

    Hattink, J.; Celis, N.; De Boeck, G.; Krijger, G.C.; Blust, R.

    2009-01-01

    Radio-caesium is an important radionuclide released considering food and feed products. In aquatic environments caesium tends to accumulate in fish, both through its diet and its gills. This presentation discusses the caesium accumulation in fish living in estuaries. The aim of this work is to conclude on the importance of potential uptake routes: via the gills, diet or ingested with water. It is suggested that the magnitude of caesium accumulation in fish is related to waterborne potassium c...

  15. Outcome of EPIZONE Extension ON VER/VNN: Pathogenicity study of 10 betanodavirus strains with an in vivo challenge in European sea bass (Dicentrarchus labrax)

    DEFF Research Database (Denmark)

    Vendramin, Niccolò; Toffan, A.; Cappellozza, E.;

    2012-01-01

    , is considered the most serious viral threat affecting marine farmed species in the Mediterranean region, thus representing one of the bottlenecks for further development of aquaculture industry. Epidemiological investigations carried out in different geographical areas demonstrated that Betanodavirus can...... be detected in wild fish as well as other aquatic organisms (artemia, rotifers, molluscs and crustaceans) in addition to farmed fish. The RGNNV genotype is the most widespread in the Mediterranean region, nevertheless some strains, characterized by containing genetic material belonging to both the RGNNV...

  16. Virulence and molecular typing of Vibrio harveyi strains isolated from cultured dentex, gilthead sea bream and European sea bass.

    Science.gov (United States)

    Pujalte, M J; Sitjà-Bobadilla, A; Macián, M C; Belloch, C; Alvarez-Pellitero, P; Pérez-Sánchez, J; Uruburu, F; Garay, E

    2003-06-01

    Vibrio harveyi was isolated from internal organs or ulcers of diseased and apparently healthy gilthead sea bream (Sparus aurata) and European sea bass (Dicentrarchus labrax) cultured in several fish farms located on the Spanish Mediterranean coast. The prevalence of the bacterium was significantly higher in European sea bass than in gilthead sea bream, and was closely related to the season in both fish species, occurring almost exclusively on warm months (June to November). After phenotypic characterization, a selection of forty five isolates from gilthead sea bream, sea bass, and several isolates previously obtained from common dentex (Dentex dentex) of the same area, were molecularly typed by automated ribotyping and random amplified polymorphic DNA (RAPD) analysis. Cluster analysis of data established 8 RAPD types and 13 ribotypes among wild isolates, and the combination of both techniques allowed to define fourteen different groups and a clear discrimination of all outbreaks and samplings. Several strains isolated from diseased gilthead sea bream and sea bass and also from asymptomatic sea bream, were tested for virulence in both fish species by intracoelomic injection. All the isolates (11) were pathogenic for sea bass, with nine out of the eleven LD50 values ranging from 1.5 x 10(5) to 1.6 x 10(6) cfu/fish. Gilthead sea bream was unaffected by the seven tested strains, even by those more virulent for sea bass, and only one strain caused a 10% mortality at 4.2 x 10(7) cfu/fish. This is the first report on virulence of V. harveyi for sea bass. PMID:12866856

  17. Fish consumption and risk of contamination by mercury – Considerations on the definition of edible parts based on the case study of European sea bass

    International Nuclear Information System (INIS)

    Highlights: ► We estimated the risk to humans trough ingestion of sea bass contaminated with Hg. ► Total and organic Hg in muscle exceeded weekly and daily safety limits. ► There’s risk through ingestion although Hg was below the maximum limit set by EU. ► The ingestion of European sea bass contaminated with Hg poses risk to human health. ► Other organs rather than muscle should be considered as edible tissue. - Abstract: In the present study, the risk to humans by consuming European sea bass (Dicentrarchus labrax), captured at three sites along a Hg contamination gradient, was evaluated by comparing muscle and kidney total Hg (T-Hg) levels with the European regulations for marketed fish. Moreover, T-Hg and organic Hg (O-Hg) levels in muscle were compared with the Provisional Tolerable Weekly Intake (PTWI) and the Reference Dose (RfD). Although T-Hg levels in muscle were below the European value allowable for marketed fish, kidney’s levels were higher than the set value, stressing the importance of redefining the concept of edible tissue and which tissues should be considered. Mercury weekly ingestion in the contaminated areas was higher than the PTWI, and O-Hg daily ingestion rates were higher than the RfD in all sampling sites. Thus, populations consuming sea bass from the contaminated sites may be at risk, with particular relevance for children and pregnant women.

  18. Gonadotropins in European sea bass: Endocrine roles and biotechnological applications.

    Science.gov (United States)

    Mazón, María José; Molés, Gregorio; Rocha, Ana; Crespo, Berta; Lan-Chow-Wing, Olivier; Espigares, Felipe; Muñoz, Iciar; Felip, Alicia; Carrillo, Manuel; Zanuy, Silvia; Gómez, Ana

    2015-09-15

    Follicle stimulating hormone (Fsh) and luteinizing hormone (Lh) are central endocrine regulators of the gonadal function in vertebrates. They act through specific receptors located in certain cell types found in the gonads. In fish, the differential roles of these hormones are being progressively elucidated due to the development of suitable tools for their study. In European sea bass (Dicentrarchus labrax), isolation of the genes coding for the gonadotropin subunits and receptors allowed in first instance to conduct expression studies. Later, to overcome the limitation of using native hormones, recombinant dimeric gonadotropins, which show different functional characteristics depending on the cell system and DNA construct, were generated. In addition, single gonadotropin beta-subunits have been produced and used as antigens for antibody production. This approach has allowed the development of detection methods for native gonadotropins, with European sea bass being one of the few species where both gonadotropins can be detected in their native form. By administering recombinant gonadotropins to gonad tissues in vitro, we were able to study their effects on steroidogenesis and intracellular pathways. Their administration in vivo has also been tested for use in basic studies and as a biotechnological approach for hormone therapy and assisted reproduction strategies. In addition to the production of recombinant hormones, gene-based therapies using somatic gene transfer have been offered as an alternative. This approach has been tested in sea bass for gonadotropin delivery in vivo. The hormones produced by the genes injected were functional and have allowed studies on the action of gonadotropins in spermatogenesis. PMID:26002037

  19. European sea bass genome and its variation provide insights into adaptation to euryhalinity and speciation

    Science.gov (United States)

    Tine, Mbaye; Kuhl, Heiner; Gagnaire, Pierre-Alexandre; Louro, Bruno; Desmarais, Erick; Martins, Rute S.T.; Hecht, Jochen; Knaust, Florian; Belkhir, Khalid; Klages, Sven; Dieterich, Roland; Stueber, Kurt; Piferrer, Francesc; Guinand, Bruno; Bierne, Nicolas; Volckaert, Filip A. M.; Bargelloni, Luca; Power, Deborah M.; Bonhomme, François; Canario, Adelino V. M.; Reinhardt, Richard

    2014-01-01

    The European sea bass (Dicentrarchus labrax) is a temperate-zone euryhaline teleost of prime importance for aquaculture and fisheries. This species is subdivided into two naturally hybridizing lineages, one inhabiting the north-eastern Atlantic Ocean and the other the Mediterranean and Black seas. Here we provide a high-quality chromosome-scale assembly of its genome that shows a high degree of synteny with the more highly derived teleosts. We find expansions of gene families specifically associated with ion and water regulation, highlighting adaptation to variation in salinity. We further generate a genome-wide variation map through RAD-sequencing of Atlantic and Mediterranean populations. We show that variation in local recombination rates strongly influences the genomic landscape of diversity within and differentiation between lineages. Comparing predictions of alternative demographic models to the joint allele-frequency spectrum indicates that genomic islands of differentiation between sea bass lineages were generated by varying rates of introgression across the genome following a period of geographical isolation. PMID:25534655

  20. ANÁLISIS DE LA SITUACIÓN ECONÓMICA-FINANCIERA DEL SECTOR PRODUCTOR DE LA DORADA (Sparus aurata L.), LUBINA (Dicentrarchus labrax L.) Y CORVINA (Argyrosomus regius A.), EN EL LITORAL MEDITERRANEO ESPAÑOL

    OpenAIRE

    BENITO ALAS, FERNANDO

    2016-01-01

    [EN] The state of the Spanish aquaculture Sea bream, Sea bass and Meagre produced in fish farms in the Spanish Mediterranean coast is analyzed setting as objective the analysis of the economical and financial situation of companies in the sector to characterize the successful companies. For this project a study was made during the period from 2002 to 2011, on the period before the economical crisis (2002-2007) and also during the crisis (2007-2011), to interpret the evolution of the compa...

  1. An oral chitosan DNA vaccine against nodavirus improves transcription of cell-mediated cytotoxicity and interferon genes in the European sea bass juveniles gut and survival upon infection.

    Science.gov (United States)

    Valero, Yulema; Awad, Elham; Buonocore, Francesco; Arizcun, Marta; Esteban, M Ángeles; Meseguer, José; Chaves-Pozo, Elena; Cuesta, Alberto

    2016-12-01

    Vaccines for fish need to be improved for the aquaculture sector, with DNA vaccines and the oral administration route providing the most promising improvements. In this study, we have created an oral chitosan-encapsulated DNA vaccine (CP-pNNV) for the nodavirus (NNV) in order to protect the very susceptible European sea bass (Dicentrarchus labrax). Our data show that the oral CP-pNNV vaccine failed to induce serum circulating or neutralizing specific antibodies (immunoglobulin M) or to up-regulate their gene expression in the posterior gut. However, the vaccine up-regulated the expression of genes related to the cell-mediated cytotoxicity (CMC; tcrb and cd8a) and the interferon pathway (IFN; ifn, mx and ifng). In addition, 3 months after vaccination, challenged fish showed a retarded onset of fish death and lower cumulative mortality with a relative survival of 45%. Thus, we created a chitosan-encapsulated DNA vaccine against NNV that is partly protective to European sea bass juveniles and up-regulates the transcription of genes related to CMC and IFN. However, further studies are needed to improve the anti-NNV vaccine and to understand its mechanisms.

  2. Sensitive periods for 17β-estradiol exposure during immune system development in sea bass head kidney.

    Science.gov (United States)

    Seemann, Frauke; Knigge, Thomas; Duflot, Aurélie; Marie, Sabine; Olivier, Stéphanie; Minier, Christophe; Monsinjon, Tiphaine

    2016-06-01

    An increasing body of evidence suggests that sex steroids play an important role in the development and regulation of vertebrate immune defense. Therefore, compounds with estrogenic activity may influence the immune system via receptor-mediated pathways. The presence of estrogen receptors in immune cells and organs during the early stages of development may indicate that female steroid hormones are involved in the maturation of the fish immune system. This is of particular importance, as some marine fish are probably exposed to sources of exogenous estrogens while they reside in their estuarine nursery grounds. In this study, the influence of 17β-estradiol (E2) on estrogen receptor and cytokine gene expression was assessed in juvenile sea bass (Dicentrarchus labrax) together with characterization of the head kidney leukocyte populations and corresponding phagocytic activity during organ regionalization from 98 to 239 dph. E2 exposure, beginning at 90 dph resulted in indirect and delayed modifications of interleukin 1β and estrogen receptor α gene expression, which may affect B-lymphocyte proliferation in the sea bass head kidney. The E2 treatment of 120 dph fish led to an increase in estrogen receptor β2 and a decrease in transforming growth factor β1 gene expression, which coincided with decreased phagocytic activity of head kidney lymphocytes and monocytes/macrophages. Additionally, these changes were observed during developmental periods described as critical phases for B-lymphocyte development in mammals. Consequently, exogenous estrogens have the potential to modify the innate immune response in juvenile sea bass and to exert detrimental effects on head kidney development. Copyright © 2015 John Wiley & Sons, Ltd. PMID:26278277

  3. Seven bass drawing, Highlanders

    OpenAIRE

    Helmlinger, Aurélie

    2011-01-01

    Seven bass drawing, Highlanders panyard, Cor. EMR William sts, Success Village, Laventille( erreur dans le nom de fichier : " highlanders_nine_bass_drawing_1" ; nom correct ="highlanders_seven_bass_drawing_1 " )

  4. Effects of rearing density on seabass (Dicentrarchus labrax, L. gene expression

    Directory of Open Access Journals (Sweden)

    M. Saroglia

    2011-03-01

    Full Text Available Animal welfare involves the subjective feeling of animals and the experience of pleasure, pain, frustration, hunger or other states and it is therefore difficult to define and to measure (Drawkins, 1990. When water quality criteria are respected and feeding restrictions are avoided, fish density may become one of the more relevant factors expected to potentially affect fish welfare. Such high density environment, may lead to a fish conditions that cannot be described or forecast simply with measurement of hematological responses (Roche and Bogé, 1996, in the same time, detection of zootechnical performances, do not provide a specific indications...

  5. Effects of dietary carbohydrate on hepatic de novo lipogenesis in European seabass (Dicentrarchus labrax L.).

    Science.gov (United States)

    Viegas, Ivan; Jarak, Ivana; Rito, João; Carvalho, Rui A; Metón, Isidoro; Pardal, Miguel A; Baanante, Isabel V; Jones, John G

    2016-07-01

    Farmed seabass have higher adiposity than their wild counterparts and this is often attributed to carbohydrate (CHO) feeding. Whether this reflects a reduction in fat oxidation, increased de novo lipogenesis (DNL), or both, is not known. To study the effects of high CHO diets on hepatic TG biosynthesis, hepatic TG deuterium ((2)H) enrichment was determined following 6 days in (2)H-enriched tank water for fish fed with a no-CHO control diet (CTRL), and diets with digestible starch (DS) and raw starch (RS). Hepatic fractional synthetic rates (FSRs, percent per day(-1)) were calculated for hepatic TG-glyceryl and FA moieties through (2)H NMR analysis. Glyceryl FSRs exceeded FA FSRs in all cases, indicating active cycling. DS fish did not show increased lipogenic potential compared to CTRL. RS fish had lower glyceryl FSRs compared with the other diets and negligible levels of FA FSRs despite similar hepatic TG levels to CTRL. DS-fed fish showed higher activity for enzymes that can provide NADPH for lipogenesis, relative to CTRL in the case of glucose-6-phosphate dehydrogenase (G6PDH) and relative to RS for both G6PDH and 6-phosphogluconate dehydrogenase. This approach indicated that elevated hepatic adiposity from DS feeding was not attributable to increased DNL. PMID:27247346

  6. Effects of oleic acid on the high threshold barium current in seabass Dicentrarchus labrax ventricular myocytes

    OpenAIRE

    Chatelier, Aurelien; Imbert, Nathalie; Zambonino, Jose-luis; McKenzie, David; Bois, P.

    2006-01-01

    The present study employed a patch clamp technique in isolated seabass ventricular myocytes to investigate the hypothesis that oleic acid (OA), a mono-unsaturated fatty acid, can exert direct effects upon whole-cell barium currents. Acute application of free OA caused a dose-dependent depression of the whole-cell barium current that was evoked by a voltage step to 0 mV from a holding potential of -80 mV. The derived 50% inhibitory concentration (IC50) was 12.49 +/- 0.27 mu mol l(-1). At a con...

  7. Hierarchical Bass model

    International Nuclear Information System (INIS)

    We propose a new model about diffusion of a product which includes a memory of how many adopters or advertisements a non-adopter met, where (non-)adopters mean people (not) possessing the product. This effect is lacking in the Bass model. As an application, we utilize the model to fit the iPod sales data, and so the better agreement is obtained than the Bass model

  8. Hierarchical Bass model

    CERN Document Server

    Tashiro, Tohru

    2013-01-01

    We propose a new model about diffusion of a product which includes a memory of how many adopters or advertisements a non-adopter met, where (non-)adopters mean people (not) possessing the product. This effect is lacking in the Bass model. As an application, we utilize the model to fit the iPod sales data, and so the better agreement is obtained than the Bass model.

  9. Live food mediated vitamin C transfer to Dicentrarchus labraxand Clarias gariepinus

    OpenAIRE

    Merchie, G.; Lavens, P.; Dhert, Ph.; Pector, R.; Mai Soni, A.F.; Nelis, H.; Ollevier, F.P.; De leenheer, A P; Sorgeloos, P.

    1995-01-01

    Live food enrichment techniques, using formulated diets and emulsions for improving the nutritional quality of Brachionus and Artemia , were studied as a tool for transferring ascorbic acid (AA) to fish larvae. Artemia nauplii enriched for 24h with an experimental emulsion containing 20% HUFA and 0%, 10% and 20% ascorbyl palmitate (AP) were administered to catfish larvae in a 20-day feeding trial. Survival was not affected by the dietary AA, but from day 7 onwards a significantly positive eff...

  10. Estudio de la respuesta inmune de la lubina Dicentrarchus labrax L. frente a mixosporidios parásitos

    OpenAIRE

    Muñoz Ruiz, Pilar

    2000-01-01

    Los objetivos de esta tesis eran realizar la caracterización antigénica de Sphaerospora dicentrarchi, un mixosporidio de las lubinas; analizar la presencia de carbohidratos en éste y otros mixosporidios; estudiar la modulación in vitro de la actividad de los leucocitos de la lubina por el parásito; y estudiar el efecto de la inmunización de lubinas con esporas de S. dicentrarchi. Los resultados demostraron la existencia de bandas en los extractos antigénicos de S. dicentrarchi con pesos molec...

  11. Effects of changes in water salinity upon exercise and cardiac performance in the European seabass ( Dicentrarchus labrax )

    DEFF Research Database (Denmark)

    Chatelier, A.; McKenzie, David; Claireaux, G.

    2005-01-01

    in seawater (SW) at a salinity of 30 parts per thousand and temperature of 14 degrees C, and their maximal sustainable (critical) swimming speed (U-crit) determined. Cardiac output (Q) was measured via an ultrasound flow probe on their ventral aorta. The fish were then exposed to acute reductions in water...... salinity, to either SW (control), 10 parts per thousand, 5 parts per thousand, or freshwater (FW, 0 parts per thousand), and their exercise and cardiac performance measured again, 18 h later. Seabass were also acclimated to FW for 3 weeks, and then their exercise performance measured before and at 18 h...... after acute exposure to SW at 30 parts per thousand. In SW, seabass exhibited an exponential increase in M-O2 and Q with increasing swimming speed, to a maximum M-O2 of 339 +/- 17 mg kg(-1) h(-1) and maximum Q of 52.0 +/- 1.9 ml min(-1) kg(-1) (mean +/- 1 SEM; n=19). Both M-O2 and Q exhibited signs...

  12. Sources of blood glucose and liver glycogen in the seabass (Dicentrarchus labrax L.): implications to carbohydrate metabolism in fish

    OpenAIRE

    Viegas, Ivan Daniel dos Santos Martins

    2012-01-01

    The Varian VNMRS 600 MHz spectrometer is supported by the National NMR Network (PTNMR) and was purchased in the framework of the National Programme for Scientific Re-equipment, contract REDE/1517/RMN/2005, with funds from POCI 2010, Fundo Europeu de Desenvolvimento Regional and Fundação para a Ciência e Tecnologia

  13. Nine bass drawing, Tropical Angel Harps

    OpenAIRE

    Helmlinger, Aurélie

    2011-01-01

    Nine bass drawing, one tone per drum, Tropical Angel Harps panyard, Southern Main Rd, Enterprise, Chaguanas (erreur sur le nom de fichier : " claytones_nine_bass_drawing_1" ; nom correct = " tropical_angel_harps_nine_bass_drawing_1")

  14. Seven bass drawing, Tropical Angel Harps

    OpenAIRE

    Helmlinger, Aurélie

    2011-01-01

    Seven bass drawing, whole tone style, Tropical Angel Harps panyard, Southern Main Rd, Enterprise, Chaguanas (erreur sur le nom de fichier : " claytones_seven_bass_drawing_1" ; nom correct = " topical_angel_harps_seven_bass_drawing_1")

  15. The Flexible Bass Absorber

    DEFF Research Database (Denmark)

    Adelman-Larsen, Niels Werner; Thompson, Eric Robert; Gade, Anders Christian

    2007-01-01

    Multi-purpose concert halls face a dilemma. They host different performance types that require significantly different acoustic conditions in order to provide the best sound quality to both the performers, sound engineers and the audience. Pop and rock music often contain high levels of bass sound...... energy but still require high definition for good sound quality. The mid- and high-frequency absorption is easily regulated, but adjusting the low-frequency absorption has typically been too expensive or requires too much space to be practical for multi-purpose halls. A practical solution to this dilemma...

  16. The Flexible Bass Absorber

    DEFF Research Database (Denmark)

    Adelman-Larsen, Niels Werner; Thompson, Eric Robert; Gade, Anders Christian

    2007-01-01

    Multi-purpose concert halls face a dilemma. They host different performance types that require significantly different acoustic conditions in order to provide the best sound quality to both the performers, sound engineers and the audience. Pop and rock music often contains high levels of bass sound...... energy but still require high definition for good sound quality. The mid- and high-frequency absorption is easily regulated, but adjusting the low-frequency absorption has typically been too expensive or requires too much space to be practical for multi-purpose halls. A practical solution to this dilemma...

  17. Black Sea Bass genetic connectivity

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Microsatellite analysis of black sea bass was undertaken to determine magnitude and direction of mixing of black seabass across the Hatteras boundary, as well as...

  18. PHYTOPLANKTON ASSEMBLAGES AT FISH FARM IN MASLINOVA BAY (THE ISLAND OF BRAČ)

    OpenAIRE

    Sanda Skejić; Živana Ninčević Gladan; Ivona Marasović

    2012-01-01

    The aim of this study was to establish phytoplankton composition at the sea bream (Sparus aurata) and sea bass (Dicentrarchus labrax) fish farm in the middle Adriatic Sea. The investigation was performed from September 2005 to September 2006 at a station located in Maslinova Bay at the island of Brač. Considering the whole research period, diatoms generally prevailed in terms of abundance while dinoflagellates were particularly abundant in June. Number of species of diatoms in comparison to d...

  19. Utilization of desalinated b rackish water resi dues for cultivation of the m a rine fish species, Dicentrarchus labra x, Sparus aurata, and Sciaenops ocellatus

    Directory of Open Access Journals (Sweden)

    Mutaz A. Al - Qutob

    2013-05-01

    Full Text Available The utilization of brackish water residues from desalination units for fish mariculture inPalestine could represent an environmental friendly alternative of disposing brine water which could havean adverse effect on wild life. In this study, three juvenile marine fish species (Gilt-head bream S. aurata, European sea bass D. labrax, and red drum S. ocellatus with average weights of 0.7-4.9 g, wereacclimated and reared in brine brackish water residues collected from Jericho desalination units withsalinities of 6.5 ‰ and 11 ‰ diluted sea water as control for 3-7 months. Fish were fed 56 % richprotein diet. Brine brackish water contained high levels of CL-1 (3369 mgL-1, Na+1(3735 mgL-1, K+1(300mgL-1, SO4 -2 (716 mgL-1 with the divalent ions Mg+2 (57.3 mgL-1 and Ca+2 (276 mgL-1.Theexperimental well showed more than the maximum allowable concentration of Cr (14.49 μgL-1, Ag (5.3μgL-1 and Mn (27.88 μgL-1 for water quality of fisheries use. The sea bass with an average weight of0.76 g (at a salinity of 6.5 ‰ showed an acceptable growth performance parameters and reached apercentage weight gain (% WG of 6345.23 % and a survival rate of 77.5 % compared to control groupsat 11 ‰ that reached at the same time a % WG of 6543.78 % and a survival rate of 82 % after 30weeks. The red drum juveniles reached a % WG of 2661.6 % and 2673.92 % after 15 weeks at 6.5 ‰and 11 ‰ respectively while sea bream reached a % WG of 241.63 % and 772.44 % after 15 weeks at6.5 ‰ and 11 ‰ respectively. Survival rate was only 5 % at both salinities for the two species. In afurther study sea bass fingerlings with an average weight of 20.5 g were reared in brackish water of 6.5‰ salinity for 7 weeks and were fed superior fish meal with fish oil. They received diets of 2.1 %, 3.0%, and 4.0 % of body weight. Sea bass fingerlings which received diets of 2.1 %, had the highest FCE(0.82 and PER (1.46 with the lowest FCR (1.22. These results confirm that the most

  20. Environmental quality for striped bass

    Energy Technology Data Exchange (ETDEWEB)

    Coutant, C. C.

    1980-01-01

    Effects of environmental changes on the quality of life for striped bass populations can be evaluated objectively with modern procedures of environmental risk analysis. Such analysis requires knowledge of the sources of risk in the context of environmental requirements of the species. A prime environmental requirement of striped bass appears to be a suitable thermal structure that accommodates the hereditary thermal niche, which changes with age. Strong thermal preferences had promoted striped bass survival in the pristine natural estuaries of eastern North America, but they may increase risks to the species in some new environments and in native ones that are altered by man. The magnitude and likelihood of risks for striped bass from many pollutants and physical changes (structures or water flow, for example) depend upon the fish's thermally controlled distribution. The importance of a species' thermal niche and of the thermal structure of aquatic environments for population success is only beginning to be recognized and included in risk assessments.

  1. Sound Generating Mechanism of the Double Bass

    Directory of Open Access Journals (Sweden)

    F. Pantelić

    2013-11-01

    Full Text Available This paper deals with the mechanical properties of the double bass and double bass bow. The sound of the instrument, as well as the vibrations of the bow, were recorded while playing. The sound was recorded with a microphone, while a sensor, placed on the bow’s stick, registered its vibrations. By observing these signals, from double bass and bow, the correlation between them was determined in order to explain the bow-string mechanism.

  2. 33 CFR 117.588 - Bass River.

    Science.gov (United States)

    2010-07-01

    ... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Bass River. 117.588 Section 117.588 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY BRIDGES DRAWBRIDGE OPERATION REGULATIONS Specific Requirements Massachusetts § 117.588 Bass River. The Hall Whitaker...

  3. Growth and nitrogen metabolism of sea bass fed graded levels of nucleic acid nitrogen from yeast or RNA extract as partial substitute for protein nitrogen from fish meal

    Directory of Open Access Journals (Sweden)

    S. Kaushik

    2010-01-01

    Full Text Available Some studies carried out in mammalian models have shown de novo synthesis and salvage of nucleotides to be a costly metabolic process and a dietary supplementation with nucleic acids (NA or nucleotides has been suggested to result in a protein sparing action (Sanderson and He, 1994. On the other hand, high levels of dietary NA could have toxic effects and lead to disturbance in protein, lipid and carbohydrate metabolism in monogastric animals lacking uricase activity, an enzyme involved in NA degradation (Clifford and Story, 1976. So far, there is no clear indication of such effects in fish fed nucleic acid-enriched diets (Tacon and Cooke, 1980; Rumsey et al., 1992; Fournier et al., 2002. The aim of this experiment was to investigate growth response and N metabolism in juvenile sea bass (D. labrax fed diets supplying graded levels of nucleic acid N from dry brewer's yeast or RNA extract as partial substitutes for protein nitrogen provided by fish meal.

  4. Diets supplemented with seaweed affect metabolic rate, innate immune, and antioxidant responses, but not individual growth rate in European seabass (Dicentrarchus labrax)

    DEFF Research Database (Denmark)

    Peixoto, Maria J.; Svendsen, Jon Christian; Malte, Hans;

    2016-01-01

    with three different diets: a control diet (CTRL), a Gracilaria-supplemented diet (GR7.5), and a mixed diet (Mix) composed of Gracilaria, Fucus, and Ulva genera representatives. All diets were isoenergetic (22 kJ g−1 adjusted for dry matter (DM)), isoproteic (47 %DM), and isolipidic (18 %DM) and tested...

  5. Chronic stress impairs performance, energy metabolism and welfare indicators in European seabass (Dicentrarchus labrax): The combined effects of fish crowding and water quality deterioration

    NARCIS (Netherlands)

    Santos, G.A.; Schrama, J.W.; Mamauag, R.E.P.; Rombout, J.H.W.M.; Verreth, J.A.J.

    2010-01-01

    Rearing fish at high densities may act as a chronic stressor leading to a reduced performance. We hypothesise that this is due to an increase in energy requirements for maintenance and altered energy partitioning. To test this hypothesis, European seabass (mean initial weight 82.1 g) were stocked at

  6. Rumble in the Jungle: City, Place and Uncanny Bass

    OpenAIRE

    Chris Christodoulou

    2011-01-01

    While bass powerfully resonates among the cultural discourses, lexicology and commercial marketing of a range of electronic dance music (EDM) styles, little popular music scholarship has paid attention to the subjective, phenomenological and psycho-physiological significance of bass in its modulation of intense feelings of pleasure. This article examines the linking in jungle/drum 'n' bass culture of bass as a sonic space that produces a powerful sense of jouissance where identity can ...

  7. Influencia de la luz y los patrones de alimentación sobre el reloj molecular, fisiología digestiva y comportamiento de dos peces teleósteos : lubina (Dicentrarchus labrax) y pez cebra (Danio rerio)= Influence of light and feeding patterns on the molecular clock, digestive physiology and behaviour of two teleost fishes: seabass (Dicentrarchus labrax) and zebrafish (Danio rerio).

    OpenAIRE

    Pozo Cano, Ana del

    2013-01-01

    La luz y los patrones de alimentación influyeron sobre el comportamiento, fisiología y reloj molecular de dos peces teleósteos. Se clonaron dos criptocromos de lubina (cry1 y cry2), expresándose en todos los tejidos estudiados y mostrando ritmos diarios de expresión en cerebro, hígado y corazón (excepto cry2). La fase (día/noche) de alimentación a demanda en lubina afecta a los patrones diarios de actividad amilasa intestinal y expresión de period1 en tejidos periféricos (i.e. hígado), pero n...

  8. Book review: Black bass diversity: Multidisciplinary science for conservation

    Science.gov (United States)

    Jelks, Howard

    2016-01-01

    These proceedings are from the third symposium dedicated to management and conservation of black basses in the genus Micropterus. The first symposium was held in 1975 (R. H. Stroud and H. Clepper. Black Bass Biology and Management. Washington (DC): Sport Fishing Institute) followed 25 years later by Black Bass 2000 (D. P. Philipp and M. S. Ridgway. 2002. Black Bass: Ecology, Conservation, and Management. Bethesda (MD): American Fisheries Society). Although the previous books discussed conservation of genetic variation and distinct strains of basses, the bulk of the papers in those tomes emphasized management of largemouth and smallmouth bass. In contrast, this third symposium is focused on the rarer bass species and challenges for their successful management.

  9. PHYTOPLANKTON COMPOSITION AT THE FISH AND SHELLFISH FARM IN THE KALDONTA BAY (CRES ISLAND)

    OpenAIRE

    Marija Tomec

    2004-01-01

    The Kaldonta Bay is situated at the south–western coast of the Cres island in the Lošinj channel, rather protected from larger influence of general sea water current. In the Bay there are installed 44 floating cages of 5 by 10 m dimensions. The cages are used for the culture of about 70 tons of sea water fish: gilthead sea bream (Sparus aurata), sea bass (Dicentrarchus labrax), sharp–snouted sparus (Diplodus puntazzo) and dentex (Dentex dentex). Besides some physico–chemical parameters (sea w...

  10. Hyperparasitism of trichodinid ciliates on monogenean gill flukes of two marine fish.

    Science.gov (United States)

    Colorni, A; Diamant, A

    2005-06-01

    Two unusual cases of hyperparasitism of trichodinid ciliates on monogenean gill flukes are described from southern Israel (Red Sea). The first case occurred in cultured European sea bass Dicentrarchus labrax infected by Diplectanum aequans, while the second was observed in a feral devil firefish Pterois miles infected by Haliotrema sp. In both cases, the trichodinids heavily co-infested the host fish gills. The flukes were completely coated by the ciliates, which gave them a cobblestone appearance, but no damage to their tegument was apparent. Both cases are most likely a result of accidental hyperparasitism, brought about by perturbed environmental conditions. PMID:16060271

  11. An Investigation of Double Bass Vibrato Characteristics

    Science.gov (United States)

    Mick, James

    2015-01-01

    The purpose of this study was to investigate and describe various vibrato characteristics of university double bass students. The primary objectives were: (1) to describe vibrato rate and width for commonly used fingers in first, fourth, and thumb positions; (2) to investigate whether students initiate vibrato in an upward or downward direction;…

  12. Effects of water velocity on activity of juvenile striped bass

    Energy Technology Data Exchange (ETDEWEB)

    Bowles, R.R.; Griffith, J.S.; Coutant, C.C.

    1976-07-01

    The swimming activity of juvenile striped bass (Morone saxatilis Walbaum) 8 to 80 mm long was investigated in a test chamber simulating, on a small scale, a fixed-screen cooling water intake structure. As water velocity increased from 0 to 30 cm/sec area and distance traveled by juvenile bass 10 to 80 mm long decreased. However, as water velocity increased from 0 to 3 cm/sec the area and distance covered by larval bass increased. The presence of food increased the activity of larval bass, but decreased the activity of juveniles. Area ranged by striped bass at test velocities ranging from 0 to 30 cm/sec increased in proportion to body length. Juvenile striped bass tested at acclimation temperatures between 20 and 5/sup 0/C experienced a 30% reduction of activity. Activity was also reduced as temperature increased from 20 to 30/sup 0/C.

  13. The fundamental thermal niche of adult landlocked striped bass

    Science.gov (United States)

    Bettoli, P.W.

    2005-01-01

    Researchers have described the temperatures selected by landlocked striped bass Morone saxatilis in different locales throughout the USA. However, seasonally low concentrations of dissolved oxygen (DO) in many systems prevented striped bass from using the cool waters (literature estimates. These results also represent the first unbiased field estimates of the influence of season on the thermal ecology of adult striped bass. The thermal characteristics of habitats considered optimal in habitat suitability index models for adult landlocked striped bass (i.e., 18-24??C) should be revised to include cooler waters. ?? Copyright by the American Fisheries Society 2005.

  14. Smallmouth bass and largemouth bass predation on juvenile Chinook salmon and other salmonids in the Lake Washington basin

    Science.gov (United States)

    Tabor, R.A.; Footen, B.A.; Fresh, K.L.; Celedonia, M.T.; Mejia, F.; Low, D.L.; Park, L.

    2007-01-01

    We assessed the impact of predation by smallmouth bass Micropterus dolomieu and largemouth bass M. salmoides on juveniles of federally listed Chinook salmon Oncorhynchus tshawytscha and other anadromous salmonid populations in the Lake Washington system. Bass were collected with boat electrofishing equipment in the south end of Lake Washington (February-June) and the Lake Washington Ship Canal (LWSC; April-July), a narrow waterway that smolts must migrate through to reach the marine environment. Genetic analysis was used to identify ingested salmonids to obtain a more precise species-specific consumption estimate. Overall, we examined the stomachs of 783 smallmouth bass and 310 largemouth bass greater than 100 mm fork length (FL). Rates of predation on salmonids in the south end of Lake Washington were generally low for both black bass species. In the LWSC, juvenile salmonids made up a substantial part of bass diets; consumption of salmonids was lower for largemouth bass than for smallmouth bass. Smallmouth bass predation on juvenile salmonids was greatest in June, when salmonids made up approximately 50% of their diet. In the LWSC, overall black bass consumption of salmonids was approximately 36,000 (bioenergetics model) to 46,000 (meal turnover consumption model) juveniles, of which about one-third was juvenile Chinook salmon, one-third was coho salmon O. kisutch, and one-third was sockeye salmon O. nerka. We estimated that about 2,460,000 juvenile Chinook salmon (hatchery and wild sources combined) were produced in the Lake Washington basin in 1999; thus, the mortality estimates in the LWSC range from 0.5% (bioenergetics) to 0.6% (meal turnover). Black bass prey mostly on subyearlings of each salmonid species. The vulnerability of subyearlings to predation can be attributed to their relatively small size; their tendency to migrate when water temperatures exceed 15??C, coinciding with greater black bass activity; and their use of nearshore areas, where overlap

  15. The importance of bass clarity in pop and rock venues

    DEFF Research Database (Denmark)

    Adelman-Larsen, Niels Werner; Thompson, Eric Robert

    2008-01-01

    High levels of bass sound have been shown to stimulate the part of the brain that controls such basic instincts as sexual desire and hunger [Todd, 2000]. In rock and pop music, the bass frequencies from 40-125 Hz get amplified to very loud levels. Easily half of the electrical power of the PA and...

  16. 76 FR 14804 - Drawbridge Operation Regulations; Bass River, Beverly, MA

    Science.gov (United States)

    2011-03-18

    ... the Hall Whitaker Bridge at mile 0.6 across the Bass River ] at Beverly, Massachusetts. The deviation.... SUPPLEMENTARY INFORMATION: The Hall Whitaker Bridge, across the Bass River at Beverly, Massachusetts, has a... this temporary deviation the Hall Whitaker Bridge may remain in the closed position from 6 p.m....

  17. THE DIATONIC ACCORDION BASS NOTES LEARNING APPLICATION

    OpenAIRE

    Koren, David

    2013-01-01

    Learning to play a musical instrument is usually tackle with the help of a music teacher. For learning purposes there already exist computer programs and applications that help the user in learning process. Many programs have a disadvantage of not showing the correct finger order while playing an instrument. In this thesis work we developed an application for an Apple iPad tablet, which teaches the user how to play the bass notes on the diatonic accordion. The presented information is based o...

  18. A Generalized Norton-Bass Model for Multigeneration Diffusion

    OpenAIRE

    Zhengrui Jiang; Dipak C. Jain

    2012-01-01

    The Norton-Bass (NB) model is often credited as the pioneering multigeneration diffusion model in marketing. However, as acknowledged by the authors, when counting the number of adopters who substitute an old product generation with a new generation, the NB model does not differentiate those who have already adopted the old generation from those who have not. In this study, we develop a generalized Norton-Bass (GNB) model that separates the two different types of substitutions. The GNB model ...

  19. NEW CAR DEMAND MODELING AND FORECASTING USING BASS DIFFUSION MODEL

    OpenAIRE

    Zuhaimy Ismail; Noratikah Abu

    2013-01-01

    Forecasting model of new product demand has been developed and applied to forecast new vehicle demand in Malaysia. Since the publication of the Bass model in 1969, innovation of new diffusion theory has sparked considerable research among marketing science scholars, operational researchers and mathematicians. The building of Bass diffusion model for forecasting new product within the Malaysian society is presented in this study. The proposed model represents the spread level of new Proton car...

  20. Rumble in the Jungle: City, Place and Uncanny Bass

    Directory of Open Access Journals (Sweden)

    Chris Christodoulou

    2011-06-01

    Full Text Available While bass powerfully resonates among the cultural discourses, lexicology and commercial marketing of a range of electronic dance music (EDM styles, little popular music scholarship has paid attention to the subjective, phenomenological and psycho-physiological significance of bass in its modulation of intense feelings of pleasure. This article examines the linking in jungle/drum 'n' bass culture of bass as a sonic space that produces a powerful sense of jouissance where identity can seem to unravel on the dance-floor and an articulation of contemporary urban space as a place of subjective loss and regression. Overlaying Freud's notion of the uncanny and Kristeva's signifying space of the chora, I discuss how this fetishisation of bass can be linked to the music's cultural formation from deindustrialised regions in London and the South-East of England during the early-1990s; its accelerated break-beats and 'dark' bass-lines can be seen to inscribe recent rapid social, cultural and environmental transformations in the urban metropolis.

  1. Assessment of endocrine disruption in Smallmouth bass (Micropterus dolomieu) and largemouth bass (Micropterus salmoides) in the Potomac River Watershed

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — Intersex, specifically testicular oocytes, has been observed in male smallmouth bass (SMB) and other centrarchids in the South Branch of the Potomac River and forks...

  2. Estudio del sistema kisspeptina y su papel en el inicio de la pubertad de la lubina europea (Dicentrarchus labrax): Efecto de la restricción calórica en machos

    OpenAIRE

    Escobar Aguirre, Sebastián

    2015-01-01

    Tesi realitzada a l'Instituto de Acuicultura de Torre de la Sal (IATS - CSIC) En esta tesis doctoral se estudia el efecto de una dieta de restricción calórica de mantenimiento a largo plazo (14 meses), en machos de lubina prepúberes, sobre el inicio y desarrollo de la pubertad. Para ello se consideró el papel que los sistemas kisspeptina/GnRH juegan en el cerebro y la gónada de esta especie en términos reproductivos. Nuestros resultados evidencian que tanto las neuronas que expresan kiss1 ...

  3. Egg saprolegniasis in a commercial sunshine bass hatchery: Control regime developed using copper sulfate

    Science.gov (United States)

    An obstacle to sunshine bass (female white bass Morone chrysops × male striped bass M. saxatilis) larval production is fungal growth on eggs caused by the water-mold Saprolegnia spp. Copper sulfate (CuSO4) is commonly used for fungus control in troughs of catfish hatcheries, but the effectiveness o...

  4. Use of copper sulfate to control Saprolegniasis at a commercial sunshine bass hatchery

    Science.gov (United States)

    An obstacle to sunshine bass (female white bass Morone chrysops × male striped bass M. saxatilis) larval production is fungal growth on eggs caused by the water-mold Saprolegnia spp. Copper sulfate (CuSO4) is commonly used for fungus control in troughs of catfish hatcheries, but the effectiveness o...

  5. Bass-SIR model for diffusion of new products

    CERN Document Server

    Fibich, Gadi

    2016-01-01

    We consider the diffusion of new products in social networks, where consumers who adopt the product can later "recover" and stop influencing others to adopt the product. We show that the diffusion is not described by the SIR model, but rather by a novel model, the Bass-SIR model, which combines the Bass model for diffusion of new products with the SIR model for epidemics. The phase transition of consumers from non-adopters to adopters is described by a non-standard Kolmogorov-Johnson-Mehl-Avrami model, in which clusters growth is limited by adopters' recovery. Therefore, diffusion in the Bass-SIR model only depends on the local structure of the social network, but not on the average distance between consumers. Consequently, unlike the SIR model, a small-worlds structure has a negligible effect on the diffusion. Surprisingly, diffusion on scale-free networks is nearly identical to that on Cartesian ones.

  6. V-moda bass freq低频耳塞

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    V-moda bass freq是一款为喜好低频的用户专门打造的耳塞.外观较为简洁。耳塞的主体单元部分采用了球状设计,表面采用了釉光处理,整体给人以珠圆玉润的感觉。由于专门为配合随声听而设计,所以bass freq非常易于驱动。

  7. Integration of Bass Enhancement and Active Noise Control System in Automobile Cabin

    Directory of Open Access Journals (Sweden)

    Liang Wang

    2008-01-01

    Full Text Available With the advancement of digital signal processing technologies, consumers are more concerned with the quality of multimedia entertainment in automobiles. In order to meet this demand, an audio enhancement system is needed to improve bass reproduction and cancel engine noise in the cabins. This paper presents an integrated active noise control system that is based on frequency-sampling filters to track and extract the bass information from the audio signal, and a multifrequency active noise equalizer to tune the low-frequency engine harmonics to enhance the bass reproduction. In the noise cancellation mode, a maximum of 3 dB bass enhancement can be achieved with significant noise suppression, while higher bass enhancement can be achieved in the bass enhance mode. The results show that the proposed system is effective for solving both the bass audio reproduction and the noise control problems in automobile cabins.

  8. Results from BASS, the BANYAN All-Sky Survey

    CERN Document Server

    Gagné, Jonathan; Doyon, René; Faherty, Jacqueline K; Malo, Lison; Artigau, Étienne

    2014-01-01

    We present results from the BANYAN All-Sky Survey (BASS), a systematic all-sky survey for brown dwarf candidates in young moving groups. We describe a cross-match of the 2MASS and AllWISE catalogs that provides a list of 98 970 potential nearby dwarfs with spectral types later than M5 with measurements of proper motion at precisions typically better than 15 mas yr$^{-1}$, as well as the Bayesian Analysis for Nearby Young AssociatioNs II tool (BANYAN II) which we use to build the BASS catalog from this 2MASS-AllWISE cross-match, consisting of more than 300 candidate members of young moving groups. We present the first results of a spectroscopic follow-up of those candidates, which allowed us to identify several new low-mass stars and brown dwarfs displaying signs of low gravity. We use the BASS catalog to show tentative evidence for mass segregation in AB Doradus and Argus, and reveal a new $\\sim$ 13 M$_{Jup}$ co-moving companion to a young low-mass star in BASS. We obtain a moderate-resolution near-infrared s...

  9. The ground-based solar observations database BASS 2000

    OpenAIRE

    Paletou, F; Lafon, M.; Maeght, P.; Grimaud, F.; Louge, T.; Aboudarham, J.

    2007-01-01

    BASS 2000 is the French solar database for ground-based instruments. We describe hereafter our organization, our tasks and the products we can deliver to the international community. Our prospects cover data mining into the THeMIS archive, a participation to the EST endeavour and the creation and curation of the ESPaDOnS/NARVAL stellar spectra database.

  10. The Bass Parasites of Oneida Lake, 80 Years Later.

    Science.gov (United States)

    Bauer, Eric F; Whipps, Christopher M

    2015-10-01

    A survey of largemouth (Micropterus salmoides) and smallmouth bass (Micropterus dolomieu) parasite communities in Oneida Lake, New York, was conducted in the summer of 2012 and compared to an earlier survey conducted by Van Cleave and Mueller during the summers of 1929 to 1931. The component helminth communities between surveys were 31% similar in composition for largemouth and 28% similar for smallmouth bass. Between species, the component helminth communities were considerably more similar in the present survey (71%) than in the survey conducted by Van Cleave and Mueller (47%). Seven species reported by Van Cleave and Mueller were present in this survey and 21 species are new records for the bass of Oneida Lake. Van Cleave and Mueller did not report prevalence values for several taxa (Monogenea, Copepoda, Myxozoa, and a Trichodina sp.) that were important for separation of parasite infracommunities in species space for both bass species. These parasites represented 28% of all species found in the current survey and may be ecologically important. Several species of parasites exhibited differences in prevalence between surveys. Two species (Rhipidocotyle papillosa and Crepidostomum cornutum) were absent from this survey but were reported as common in the 1929-1931 survey and almost certainly represent extirpations that coincide with the loss of their native bivalve hosts from Oneida Lake. Other differences in the parasite communities may also be explained by the ecological disturbances in Oneida Lake over the past 81 yr. The changes in bass parasite communities between surveys emphasize the importance of recognizing the historical nature of parasite communities, especially in ecosystems with a history of large-scale changes. Most importantly our findings suggest that, similar to trends observed in free-living freshwater biotic communities, anthropogenic ecosystem disturbances may homogenize fish parasite communities.

  11. Seasonal changes in community composition and trophic structure of fish populations of five salt marshes along the Essex coastline, United Kingdom

    Science.gov (United States)

    Green, Benjamin C.; Smith, David J.; Earley, Sarah E.; Hepburn, Leanne J.; Underwood, Graham J. C.

    2009-11-01

    European intertidal salt marshes are important nursery sites for juvenile fish and crustaceans. Due to the increasing threat of habitat loss, the seasonal changes of salt marsh fish communities need to be understood in order to appreciate the ecological and economic importance of the saltmarsh habitat. This study was the first in Great Britain to investigate the seasonal changes of salt marsh fish communities and the variation in community structure between closely located marsh habitats. Between February 2007 and March 2008, five marshes on three estuaries of the Essex coastline were sampled using flume nets to block off intertidal creeks at high tide. Fourteen fish species were caught. The community overall was dominated by three species that made up 91.6% of the total catch: the common goby Pomatoschistus microps (46.2% of the total catch), juvenile herring Clupea harengus (24.3%), and juvenile and larval sea bass Dicentrarchus labrax (21.2%). Cluster analysis demonstrated clear seasonal patterns, with some community structures unique to specific marshes or estuaries. The marsh fish community shifts from a highly diverse community during spring, to a community dominated by D. labrax and P. microps in autumn, and low diversity during winter months. Gravimetric stomach content analysis of fish community identified three main trophic guilds; macroinvertivores, planktivores and omnivores. The macroinvertivore feeding guild contained D. labrax and P. microps, the two most frequently occurring species. This investigation demonstrates the importance of British salt marshes as nursery habitats for commercial fish species.

  12. Comparing catch orientation among Minnesota walleye, northern pike, and bass anglers

    Science.gov (United States)

    Schroeder, Susan A.; Fulton, David C.

    2013-01-01

    We compared the catch orientations of Minnesota walleye (Sander vitreus), northern pike (Esox lucius), largemouth bass (Micropterus salmoides), and smallmouth bass (Micropterus dolomieu) anglers. Results were derived from 2009, 2010, and 2012 surveys of anglers targeting these different species. Consistent with previous research, we identified four dimensions of anglers’ catch orientation: (a) catching something, (b) catching big fish, (c) catching many fish, and (d) keeping fish. Walleye anglers were the most motivated to keep fish, while northern pike anglers were more oriented toward catching big fish. Largemouth bass anglers, and to a lesser extent smallmouth bass anglers, were also oriented toward catching big fish. Bass anglers reported the lowest interest in keeping fish. An orientation to keep fish was negatively related to more restrictive management actions, regardless of species. A stronger orientation to catch big fish was associated with support for increased harvest restrictions only for northern pike and smallmouth bass.

  13. Teaching Fourier Analysis and Wave Physics with the Bass Guitar

    CERN Document Server

    Courtney, M; Courtney, Michael; Althausen, Norm

    2006-01-01

    This article describes a laboratory or demonstration technique employing the bass guitar and a Vernier LabPro (or a PC soundcard) for teaching wave physics and introducing Fourier analysis. The Fourier transform of an open string provides a demonstration of oscillatory modes out to the 20th harmonic consistent with expectations containing a fundamental frequency and harmonics. The playing of "harmonics" (suppressing resonant modes by lightly touching the string to enforce nodes at desired locations) demonstrates oscillations made up (mostly) of individual modes. Students see that the complete set of Fourier components (fundamental and harmonics) present on the open string can be explicitly connected with individual resonant frequencies as described in typical textbook discussions of natural frequencies of waves on a string. The use of a bass guitar rather than the six string electric guitar allows higher harmonics to be individually excited, and it is also easier for students to play the harmonics themselves.

  14. Topological Hochschild homology and the Bass trace conjecture

    OpenAIRE

    Berrick, A. J.; Hesselholt, Lars

    2013-01-01

    We use the methods of topological Hochschild homology to shed new light on the groups satisfying the Bass trace conjecture. We show that the factorization of the Hattori-Stallings rank map through the Bokstedt-Hsiang-Madsen cyclotomic trace map leads to Linnell's restriction on such groups. As a new consequence of this restriction, we show that the conjecture holds for any group G with the property that every subgroup that is isomorphic to the additive group of rational numbers has nontrivial...

  15. FAST Real Time PCR for control of intra-species recycling in aquaculture feed, focused to the most relevant fish species farmed in Europe.

    Science.gov (United States)

    Espiñeira, Montserrat; Vieites, Juan M

    2016-08-01

    Recent regulations in animal feed composition prohibit intra-species recycling, the recycling of one given animal species to the same species, in order to avoid potential safety risks to human and animal health. These regulations have generated the need of their control in aquaculture by effective and specific analytical techniques. To date, most studies of species identification and detection in feedstuffs are focused on land species, but few studies are focused on species composition in fish feed. The present work describes five methodologies based in Real Time PCR for detection of the most relevant fish species farmed in Europe: gilthead sea bream (Sparus aurata); sea bass (Dicentrarchus labrax); turbot (Scophthalmus maximus); rainbow trout (Onchorynchus mykiss); and salmon (Salmo salar), in order to guarantee the intra-species recycling regulation in aquaculture feedstuffs. PMID:26988512

  16. “AquaTrace” The development of tools for tracing and evaluating the genetic impact of fish from aquaculture

    DEFF Research Database (Denmark)

    Eg Nielsen, Einar; Bekkevold, Dorte; Svåsand, Terje;

    2012-01-01

    Aquaculture represents a key solution to meet the escalating demand for fish. Accordingly, development of appropriate legislation within the European Union aquaculture sector underpinned by cutting‐edge research and technology is required. This necessitates implementation of breeding programmes...... to identify of the genetic origin of both wild and farmed fish (assignment and genetic traceability), as well as for the detection of interbreeding genetic introgression between farmed and wild stocks. This work will be carried out on three marine fish of economic significance: the European sea bass...... (Dicentrarchus labrax), gilthead sea bream (Sparus aurata), and turbot (Scophthalmus maximus). To address quantitative effects of farm introgression, the rationale is to examine links between key fitness and life‐history traits and specific functional genetic variation between wild and farmed fish, using...

  17. The BASS survey for brown dwarfs in young moving groups

    Science.gov (United States)

    Gagne, Jonathan; Lafreniere, David; Doyon, Rene; Malo, Lison; Faherty, Jacqueline K.; Artigau, Etienne; Cruz, Kelle L.; Burgasser, Adam J.; Filippazzo, Joe; Naud, Marie-Eve; Albert, Loic; Bouchard, Sandie; Gizis, John; Robert, Jasmin; Nadeau, Daniel; Bowsher, Emily C.; Nicholls, Christine

    2016-01-01

    I will present in this dissertation talk the construction and follow-up of the BANYAN All-Sky Survey (BASS) that we led to identify dozens of new isolated young brown dwarfs in the Solar neighborhood, several of which have physical properties such as mass, age and temperature that make them similar to exoplanets that were recently discovered using the method of direct imaging.Such isolated analogs of the giant, gaseous exoplanets are precious benchmarks that will allow a deep characterization of their atmospheres using high-resolution and high signal-to-noise spectroscopy, which is made possible due to the absence of a nearby and bright host star.I will end by describing BASS-Ultracool, an extension of BASS that focuses on the identification of extremely cool isolated exoplanet analogs that display methane in their atmospheres. This survey has already uncovered the first bonafide T dwarf member of a moving group, the ~150 Myr AB Doradus T5, SDSS1110+0116.

  18. C-BASS: The C-Band All Sky Survey

    Science.gov (United States)

    Pearson, Timothy J.; C-BASS Collaboration

    2016-06-01

    The C-Band All Sky Survey (C-BASS) is a project to image the whole sky at a wavelength of 6 cm (frequency 5 GHz), measuring both the brightness and the polarization of the sky. Correlation polarimeters are mounted on two separate telescopes, one at the Owens Valley Observatory (OVRO) in California and another in South Africa, allowing C-BASS to map the whole sky. The OVRO instrument has completed observations for the northern part of the survey. We are working on final calibration of intensity and polarization. The southern instrument has recently started observations for the southern part of the survey from its site at Klerefontein near Carnarvon in South Africa. The principal aim of C-BASS is to allow the subtraction of polarized Galactic synchrotron emission from the data produced by CMB polarization experiments, such as WMAP, Planck, and dedicated B-mode polarization experiments. In addition it will contribute to studies of: (1) the local (NASA) in the USA, the Hartebeesthoek Radio Astronomy Observatory (supported by the Square Kilometre Array project) in South Africa, and the King Abdulaziz City for Science and Technology (KACST) in Saudi Arabia.

  19. NEW CAR DEMAND MODELING AND FORECASTING USING BASS DIFFUSION MODEL

    Directory of Open Access Journals (Sweden)

    Zuhaimy Ismail

    2013-01-01

    Full Text Available Forecasting model of new product demand has been developed and applied to forecast new vehicle demand in Malaysia. Since the publication of the Bass model in 1969, innovation of new diffusion theory has sparked considerable research among marketing science scholars, operational researchers and mathematicians. The building of Bass diffusion model for forecasting new product within the Malaysian society is presented in this study. The proposed model represents the spread level of new Proton car among a given set of the society in terms of a simple mathematical function that elapsed since the introduction of the new car. With the limited amount of data available for the new car, a robust Bass model was developed to forecast the sales volume. A procedure of the proposed diffusion model was designed and the parameters were estimated. Results obtained by applying the proposed model and numerical calculation shows that the proposed diffusion model is robust and effective for forecasting demand of new Proton car. The proposed diffusion model is shown to forecast more effectively and accurately even with insufficient previous data on the new product.

  20. An Investigation into the Uptake of Contaminants in Largemouth Bass and Sediment from the Lower Tombigbee and Mobile Rivers, 1992

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This report summarizes a study with the following results: 1. Mercury was detected in all largemouth bass composite samples, with highest levels found in bass...

  1. Caspase-1 and IL-1β processing in a teleost fish.

    Directory of Open Access Journals (Sweden)

    Marta I R Reis

    Full Text Available Interleukine-1β (IL-1β is the most studied pro-inflammatory cytokine, playing a central role in the generation of systemic and local responses to infection, injury, and immunological challenges. In mammals, IL-1β is synthesized as an inactive 31 kDa precursor that is cleaved by caspase-1 generating a 17.5 kDa secreted active mature form. The caspase-1 cleavage site strictly conserved in all mammalian IL-1β sequences is absent in IL-1β sequences reported for non-mammalian vertebrates. Recently, fish caspase-1 orthologues have been identified in sea bass (Dicentrarchus labrax and sea bream (Sparus aurata but very little is known regarding their processing and activity. In this work it is shown that sea bass caspase-1 auto-processing is similar to that of the human enzyme, resulting in active p24/p10 and p20/p10 heterodimers. Moreover, the presence of alternatively spliced variants of caspase-1 in sea bass is reported. The existence of caspase-1 isoforms in fish and in mammals suggests that they have been evolutionarily maintained and therefore are likely to play a regulatory role in the inflammatory response, as shown for other caspases. Finally, it is shown that sea bass and avian IL-1β are specifically cleaved by caspase-1 at different but phylogenetically conserved aspartates, distinct from the cleavage site of mammalian IL-1β.

  2. High density culture of white bass X striped bass fingerlings in raceways using power plant heated effluent

    Energy Technology Data Exchange (ETDEWEB)

    Collins, C.M.; Burton, G.L.; Schweinforth, R.L.

    1983-06-01

    White bass (Morone chrysops) X striped bass (M. saxatilis) hybrids weighing 1691/lb were initially stocked in five 24 ft/sup 3/ floating screen cages for 20 days. Hybrids averaging one inch in total length and 361 fish/lb were released in four 614 ft/sup 3/ concrete raceways. Two stocking densities, 2.6 and 5.1 fish/ft/sup 3/, were evaluated in the 94-day study using a flow rate of 300 gpm/raceway. Water temperatures averaged 79/sup 0/F and water quality was adequate throughout the production period. Fish were hand fed to satiation daily. Columnaris and Aeromonas hydrophila caused the most serious disease problems. Gas supersaturation was suspect in high mortality levels during cage culture of hybrid bass fry. Cannibalism may have been responsible for unaccountable losses prior to raceway stocking and at harvest. The study yielded 5773 hybrids weighing 658 lb. The high density treatment showed greater weight gain, average weight, average length and percent survival as well as improved food conversion. Results suggest that higher stocking densities and periodic grading may increase production and suppress cannibalism. 10 references, 3 figures, 3 tables.

  3. Reproducción natural controlada del black bass Micropterus salmoides - Controlled natural reproduction of black bass Micropterus salmoides

    Directory of Open Access Journals (Sweden)

    Amaral Junior, Hilton

    2009-09-01

    Full Text Available ResumenPara viabilizar la reproducción, larvicutura y alevinaje del Black bass, en la región sur de Brasil, se ha buscado adaptar una tecnología, desarrollada por el Centro Nacional de Acuicultura Piscifactoría las Vegas del Guadiana Badajoz/España. Consiste en controlar el ambiente de desove, utilizando estanques con nidales, para el desove del Black bass. Optimizando esta tecnología, desarrollamos una técnica de adaptación del Black bass al cultivo intensivo durante el período de invierno, ofreciendo un pienso de un 40% de proteína cruda y zooplancton (Daphnia sp.. Se dejan los reproductores en un mismo estanque de engorda en sistema extensivo, y son adaptados para un sistema intensivo de cultivo, en estanques de hormigón con entrada y salida del agua. Se acondicionan reproductores machos y hembras, durante los meses de invierno. En la primavera, se abren sucesivamente las compuertas de comunicación del departamentocentral, con los estanques frezaderos. En estos estanques frezaderos,se colocan nidales de grava para la puesta de las hembras. Pasado elperíodo de ocupación cíclica de los estanques de desove, losreproductores vuelven al departamento central. Después de laeclosión, las larvas son alimentadas con pienso molido a un 48% deproteína cruda con una oferta de un 10% de su peso y zooplancton Daphina sp. Los alevines se quedan alojados en estanques de tierrapara el período de engorda.SummaryIn order to make reproduction, larval and fingerling rearing of BlackBass in the south region of Brazil, we try to adapt a technologydeveloped by Centro Nacional de Aquicultura Piscifactoria Las Vegasdel Guadiana, Badajoz, Espanha. The technique consists in controllingthe spawning environment with a tank containing nests. To optimizethis technology, we develop a technique to adapt the black bass in aintensive culture during winter, providing food with 40% proteine andzooplankton (Daphnia sp.. The spawners are manteined in the

  4. Osmoregulatory effects of hypophysectomy and homologous prolactin replacement in hybrid striped bass

    DEFF Research Database (Denmark)

    Jackson, Leslie F; McCormick, Stephen D; Madsen, Steffen S;

    2005-01-01

    The effects of ovine prolactin (oPRL) and striped bass prolactin (sbPRL; Morone saxatilis) on plasma osmolality, electrolyte balance, and gill Na+,K+-ATPase activity were investigated in hypophysectomized (Hx), freshwater (FW)-acclimated, hybrid striped bass (M. saxatilis x Morone chrysops...

  5. Comparative severity of experimentally induced mycobacteriosis in striped bass Morone saxatilis and hybrid tilapia Oreochromis spp.

    Science.gov (United States)

    Wolf, J C; Smith, S A

    1999-11-30

    Twenty striped bass Morone saxatilis and 20 hybrid tilapia Oreochromis niloticus x O. mossambicus x O. aureus each received a single intramuscular injection of 1.6 x 10(6) colony forming units per gram body weight of Mycobacterium marinum. Striped bass manifested significantly greater clinical and microscopic disease compared to tilapia. Whereas all the striped bass had died or were clinically ill by Day 8 post-infection, there was no apparent disruption of normal behaviour, physical appearance, or growth in any of the sacrificed or surviving tilapia. Histologically, granulomas in striped bass were generally larger and less discrete, with a higher proportion of heavily vacuolated macrophages, and large cores of necrotic cells. Visceral granulomas in tilapia were smaller, with a higher proportion of epithelioid macrophages, more pigment-containing cells, more peripheral lymphocytes, and virtually no central necrosis. Visceral granulomas were 18-fold more numerous in striped bass than in tilapia. Based upon histomorphometric data, mean proportions of acid-fast bacteria within pronephros granulomas were 4-fold greater in striped bass than tilapia, and striped bass granulomas averaged more than twice as large as tilapia granulomas. In the anterior kidney of striped bass, a positive correlation existed between mean mycobacterial proportions and mean necrosis scores. In tilapia, mean mycobacterial proportions correlated negatively with mean granuloma numbers, whereas there was no correlation between these parameters in striped bass. Results suggest that intrinsic functional differences in the immunologic systems of striped bass and hybrid tilapia may contribute to inter-species variation in mycobacteriosis susceptibility. PMID:10686670

  6. Mycobacterial infections in striped bass from Delaware Bay

    Science.gov (United States)

    Ottinger, C.A.; Brown, J.J.; Densmore, Christine L.; Starliper, C.E.; Blazer, V.S.; Weyers, H.S.; Beauchamp, K.A.; Rhodes, M.W.; Kator, H.; Gauthier, David T.; Vogelbein, W.K.

    2007-01-01

    Eighty striped bass Morone saxatilis were obtained from Delaware Bay using commercial gill nets set adjacent to Woodland Beach (n = 70) and Bowers Beach (n = 10) in December 2003. Fish were examined for gross lesions. Total lengths (TLs) and eviscerated weights were determined to calculate condition factors (K). Portions of spleens were aseptically harvested for bacterial culture, and portions of spleens, kidneys (anterior and posterior), livers, and gonads were obtained for histological examination. The size distribution of the striped bass was relatively homogeneous; the mean TL was about 600 mm for all samples. Mean K exceeded 0.95 in all samples and was not significantly different (P > 0.05) among samples. Significant differences in mycobacterial infection prevalence (P ??? 0.05) were observed among samples; samples obtained at Woodland Beach (WB) on December 10 (53.8%, n = 13) and December 17 (7.1%, n = 42) exhibited the most striking differences in prevalence. Mycobacterial infection intensity ranged from 1 ?? 102 to 1 ?? 107 colony-forming units per gram of spleen. Acanthocephalan infection prevalence and intensity, non-acid-fast bacterial infection prevalence, and fish sex ratio were also significantly different among the samples (P ??? 0.05). Similar to the mycobacterial infections, differences in sex ratio, acanthocephalan infection, and non-acid-fast bacterial infection were observed between the WB samples taken on December 10 and 17. However, no significant associations (P > 0.05) were observed between sex ratio or these infections and mycobacterial infection. The differences in bacterial and parasite infection prevalence and intensity and fish sex ratio in some samples indicate that these fish had a different history and that the epizootiology of mycobacterial infection in striped bass from Delaware Bay may be relatively complex. ?? Copyright by the American Fisheries Society 2007.

  7. Four types of neoplasms in Asian sea bass (Lates calcarifer)

    Institute of Scientific and Technical Information of China (English)

    Ramalingam Vijayakumar; Kuzhanthaivel Raja; Vijayapoopathi Singaravel; Ayyaru Gopalakrishnan

    2015-01-01

    Objective:To describe and observe four types of neoplasms on different parts (external and internal organs) of an Asian sea bass (Lates calcarifer). Methods:The sample was collected from local fish landing center (south east coast of India). Histopathology of normal and tumour tissues were analyzed. Results:A total of 83 tumour masses (neoplasm) were recorded on the fish skin, also the neoplasms were recorded in internal organs of fish such as liver, stomach and ovary. Conclusions:Aetiology of such neoplasm’s are unknown, further more researches need to confirm the causative agent for this type of neoplasm.

  8. Raynaud´s phenomenon in a slap bass player

    DEFF Research Database (Denmark)

    Jepsen, Jørgen Riis; Simonsen, Jane Angel

    2016-01-01

    OBJECTIVE: Secondary Raynaud’s phenomenon is a frequent condition related to occupational exposure to local vibration but has not been described in musicians. This study aims to describe cold-induced blanching of the right second and (in particular) third digits in a 67-year-old double bass player...... was immeasurable at the finger level, corresponding to a finger/brachial index of 0% of the second and third fingers. CONCLUSION: This is the first reported case of objectively verified, playing-related Raynaud’s phenomenon in a musician....

  9. Testing the thermal-niche oxygen-squeeze hypothesis for estuarine striped bass

    Science.gov (United States)

    Kraus, Richard T.; Secor, D.H.; Wingate, Rebecca L.

    2015-01-01

    In many stratified coastal ecosystems, conceptual and bioenergetics models predict seasonal reduction in quality and quantity of fish habitat due to high temperatures and hypoxia. We tested these predictions using acoustic telemetry of 2 to 4 kg striped bass (Morone saxatilis Walbaum) and high-resolution spatial water quality sampling in the Patuxent River, a sub-estuary of the Chesapeake Bay, during 2008 and 2009. Striped bass avoided hypoxic (dissolved oxygen ≤2 mg·l−1) subpycnocline waters, but frequently occupied habitats with high temperatures (>25 °C) in the summer months, as cooler habitats were typically not available. Using traditional concepts of the seasonal thermal-niche oxygen-squeeze, most of the Patuxent estuary would beconsidered unsuitable habitat for adult striped bass during summer. Application of a bioenergetics model revealed that habitats selected by striped bass during summer would support positive growth rates assuming fish could feed at one-half ofmaximum consumption. Occupancy of the estuary during summer by striped bass in this study was likely facilitated by sufficient prey and innate tolerance of high temperatures by sub-adult fish of the size range that we tagged. Our results help extend the thermalniche oxygen-squeeze hypothesis to native populations of striped bass in semi-enclosed coastal systems. Tolerance of for supraoptimal temperatures in our study supports recent suggestions by others that the thermal-niche concept for striped bass should be revised to include warmer temperatures.

  10. Striped Bass Spawning in Non-Estuarine Portions of the Savannah River

    Energy Technology Data Exchange (ETDEWEB)

    Martin, D.; Paller, M.

    2007-04-17

    Historically, the estuarine portions of the Savannah River have been considered to be the only portion of the river in which significant amounts of striped bass (Morone saxatilis) spawning normally occur. A reexamination of data from 1983 through 1985 shows a region between River Kilometers 144 and 253 where significant numbers of striped bass eggs and larvae occur with estimated total egg production near that currently produced in the estuarine reaches. It appears possible that there are two separate spawning populations of striped bass in the Savannah River.

  11. Otolith Sr concentration analyzed by PIXE in Ariake estuary-dependent sea bass juveniles

    International Nuclear Information System (INIS)

    Japanese sea bass (Lateolabrax japonicus) is a typical euryhaline marine fish and frequently migrates from salt to freshwater environments during early life stages. We hypothesized that strontium concentrations in the otolith could be a useful index to examine freshwater entry because of its lower concentration in freshwater. Otoliths of Japanese sea bass juveniles collected in the Chikugo river and estuary were analyzed by Particle Induced X-ray Emission (PIXE) to see relationship between strontium concentration and ambient salinity. Strontium concentrations in otoliths of sea bass juveniles are significantly lower in the river samples than in brackish water samples. (author)

  12. Eggs Incubation, Early Development and growth in Frys of Brown Trout (Salmo trutta macrostigma) and Black Sea Trout (Salmo trutta labrax)

    OpenAIRE

    Ahmet ALP; Erer, Mesut; KAMALAK, Adem

    2010-01-01

    Some of the embryonic development stages and growth of the frys of brown trout (Salmo trutta macrostigma) and Black Sea trout (Salmo trutta labrax) were examined. The first eye pigmentation in the brown trout eggs were realised on the 35 days (244 day-degree) at 7.23°C and 31 days (260 day-degree) at 8.21°C post fertilisation. First eye pigmentation of the Black Sea trout eggs were seen on day 25 (215 day-degree) at 8.21°C. Brown trout larvae were hatched on day 56 (387 day-degree) at 7.23°C ...

  13. Mercury concentrations in largemouth bass and other fishes of the Loxahatchee National Wildlife Refuge

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — From September 17-21, 1990, 32 largemouth bass (Micropterus salmoides) and three other fish species were collected from eight locations at the Loxahatchee National...

  14. Siim Nestor soovitab : Back2Bass Helsinki. Teenage Kicks / Siim Nestor

    Index Scriptorium Estoniae

    Nestor, Siim, 1974-

    2002-01-01

    Soome trummi-ja-bassi klubi Back2Bass 28. septembril KuKu klubis Tallinnas. 28. sept. Von Krahlis toimuvast live-üritusest Teenage Kicks, kus ansambel Claire's Birthaday esitleb ka oma uut singlit "Do You Remember"

  15. Contaminants in striped bass from the flint and Apalachicola Rivers 1986-1989

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — Five striped bass (Morone saxitilis), collected between April 1986 and May 1989 from the Flint River at Albany, Georgia, and the Apalachicola River at...

  16. Mercury concentrations in largemouth bass of the Crystal River National Wildlife Refuge

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — From July 17 to 26, 1990, 20 largemouth bass (Micropterus salmoides) were collected from four locations at the Crystal River National Wildlife Refuge, Citrus...

  17. Mercury in largemouth bass of the Lake Woodruff National Wildlife Refuge

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — From August 24 to 28, 1990, largemouth bass (Micropterus salmoides) were collected from selected locations at the Lake Woodruff National Wildlife Refuge, Volusia...

  18. Mercury in largemouth bass and other fishes of the Merritt Island National Wildlife Refuge

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — From August 16 to 22, 1990, largemouth bass (Micropterus salmoides), bowfin (Amia calva) and spotted seatrout (Cynoscion nebulosus) were collected from selected...

  19. Ocean acidification impacts on black sea bass and scup embryos, responses of finfish in laboratory experiments

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Black sea bass (Centropristis striata) and scup (Stenotomus chrysops) compose important recreational and commercial fisheries along the United States Atlantic...

  20. Mercury in largemouth bass and spotted gar of the Florida Panther National Wildlife Refuge

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — From June 21 to 25, 1990, 21 largemouth bass (Micropterussa salmoides) and five spotted gar (Lepisosteus oculatus) were collected from selected locations at the...

  1. A STUDY ON NEW PRODUCT DEMAND FORECASTING BASED ON BASS DIFFUSION MODEL

    OpenAIRE

    Zuhaimy Ismail; Noratikah Abu

    2013-01-01

    A forecasting model of new product demand has been developed and applied to forecast new vehicle demand in Malaysia. Since the publication of the Bass model in 1969, innovation of new diffusion theory has sparked considerable research among marketing science scholars, operational researchers and mathematicians. This study considers the Bass Model for forecasting the diffusion of new products or an innovation in the Malaysian society. The objective of the proposed model is to represent the lev...

  2. A Generalized Bass Model for Product Growth in Networks

    CERN Document Server

    Manshadi, Vahideh H

    2016-01-01

    Many products and innovations become well-known and widely adopted through the social interactions of individuals in a population. The Bass diffusion model has been widely used to model the temporal evolution of adoption in such social systems. In the model, the likelihood of a new adoption is proportional to the number of previous adopters, implicitly assuming a global (or homogeneous) interaction among all individuals in the network. Such global interactions do not exist in many large social networks, however. Instead, individuals typically interact with a small part of the larger population. To quantify the growth rate (or equivalently the adoption timing) in networks with limited interactions, we study a stochastic adoption process where the likelihood that each individual adopts is proportional to the number of adopters among the small group of persons he/she interacts with (and not the entire population of adopters). When the underlying network of interactions is a random $k$-regular graph, we compute t...

  3. Helminth parasites of fish and shellfish from the Santa Gilla Lagoon in southern Sardinia, Italy.

    Science.gov (United States)

    Culurgioni, J; Sabatini, A; De Murtas, R; Mattiucci, S; Figus, V

    2014-12-01

    An extensive survey of helminth parasites in fish and shellfish species from Santa Gilla, a brackish water lagoon in southern Sardinia (western Mediterranean), resulted in the identification of 69 helminth parasite taxa and/or species from 13 fish species (n= 515) and seven bivalve species (n= 2322) examined between September 2001 and July 2011. The list summarizes information on the helminth parasites harboured by fish and molluscs contained in the available literature. Digenea species (37), both adults and larvae, dominated the parasite fauna, whereas Cestoda were the least represented class (three species). Monogenea, Nematoda and Acanthocephala were present with 17, 6 and 6 species, respectively, which were mainly adults. The most widespread parasite species was the generalist Contracaecum rudolphii A (Nematoda). Other species, such as the Haploporidae and Ascocotyle (Phagicola) spp. 1 and 2 (Digenea), showed a high family specificity in Mugilidae. Importantly, the study recorded the occurrence of potential zoonotic agents, such as Heterophyes heterophyes, Ascocotyle (Phagicola) spp. and C. rudolphii A, the latter two reaching the highest indices of infection in the highly marketed fish grey mullet and sea bass, respectively. The highest parasite richness was detected in Dicentrarchus labrax, which harboured 17 helminth species, whereas the lowest value was observed in Atherina boyeri, infected by only three species. The list includes the first geographical record in Italian coastal waters of Robinia aurata and Stictodora sawakinensis, and 30 reports of new host-parasite complexes, including the larval stages of Ascocotyle (Ascocotyle) sp. and Southwellina hispida in D. labrax.

  4. Behavioural thermoregulation and bioenergetics of riverine smallmouth bass associated with ambient cold-period thermal refuge

    Science.gov (United States)

    Westhoff, J.T.; Paukert, Craig P.; Ettinger-Dietzel, Sarah; Dodd, H.R.; Siepker, Michael

    2016-01-01

    Smallmouth bass in thermally heterogeneous streams may behaviourally thermoregulate during the cold period (i.e., groundwater temperature greater than river water temperature) by inhabiting warm areas in the stream that result from high groundwater influence or springs. Our objectives were to determine movement of smallmouth bass (Micropterus dolomieu) that use thermal refuge and project differences in growth and consumption among smallmouth bass exhibiting different thermal-use patterns. We implanted radio transmitters in 29 smallmouth bass captured in Alley Spring on the Jacks Fork River, Missouri, USA, during the winter of 2012. Additionally, temperature archival tags were implanted in a subset of nine fish. Fish were tracked using radio telemetry monthly from January 2012 through January of 2013. The greatest upstream movement was 42.5 km, and the greatest downstream movement was 22.2 km. Most radio tagged fish (69%) departed Alley Spring when daily maximum river water temperature first exceeded that of the spring (14 °C) and during increased river discharge. Bioenergetic modelling predicted that a 350 g migrating smallmouth bass that used cold-period thermal refuge would grow 16% slower at the same consumption level as a fish that did not seek thermal refuge. Contrary to the bioenergetics models, extrapolation of growth scope results suggested migrating fish grow 29% more than fish using areas of stream with little groundwater influence. Our results contradict previous findings that smallmouth bass are relatively sedentary, provide information about potential cues for migratory behaviour, and give insight to managers regarding use and growth of smallmouth bass in thermally heterogeneous river systems.

  5. Seasonal use of a New England estuary by foraging contingents of migratory striped bass

    Science.gov (United States)

    Mather, Martha E.; Pautzke, Sarah M.; Finn, John T.; Deegan, Linda A.; Muth, Robert M.

    2011-01-01

    Using acoustic telemetry on migratory striped bass Morone saxatilis in Plum Island Estuary (PIE), Massachusetts, we found that striped bass (335–634 mm total length) tagged in the spring and summer of 2005 (n = 14) and 2006 (n = 46) stayed in the estuary for an average of 66.0 d in 2005 and 72.2 d in 2006. Striped bass spent the most time in two specific reaches: middle Plum Island Sound and lower Rowley River. In both years, three different use-groups of striped bass were observed in PIE. Short-term visitors (n = 24) stayed in the estuary only briefly (range = 5–20 d). Two groups of seasonal residents stayed for more than 30 d, either in the Rowley River (n = 14) or in Plum Island Sound (n = 22). Within PIE, the two seasonal-resident use-groups may be foraging contingents that learn how to feed efficiently in specific parts of the estuary. These distinct within-estuary use patterns could have different implications for striped bass condition and prey impact.

  6. A STUDY ON NEW PRODUCT DEMAND FORECASTING BASED ON BASS DIFFUSION MODEL

    Directory of Open Access Journals (Sweden)

    Zuhaimy Ismail

    2013-01-01

    Full Text Available A forecasting model of new product demand has been developed and applied to forecast new vehicle demand in Malaysia. Since the publication of the Bass model in 1969, innovation of new diffusion theory has sparked considerable research among marketing science scholars, operational researchers and mathematicians. This study considers the Bass Model for forecasting the diffusion of new products or an innovation in the Malaysian society. The objective of the proposed model is to represent the level of spread on new products among a given set of society in terms of a simple mathematical function that elapsed since the introduction of new products. With limited amount of data available for new products, a robust Bass model was developed to forecast the sales volume. A procedure of the proposed diffusion model was designed and the parameters were estimated. Results obtained by applying the proposed model and numerical calculation show that the proposed Bass diffusion model is robust and effective for forecasting demand of new products. This study concludes that the newly developed bass diffusion of demand function has significantly contributed for forecasting the diffusion of new products.

  7. Population structure and dynamics of northern pike and smallmouth bass in Coeur d’Alene Lake, Idaho.

    Science.gov (United States)

    Walrath, John D.; Quist, Michael; Firehammer, Jon A.

    2015-01-01

    Numerous species have been introduced to Coeur d'Alene Lake, Idaho over the last century, but minimal research has been completed to understand their population dynamics. The objective of this study was to describe the population demographics and dynamics of northern pike (Esox lucius) and smallmouth bass (Micropterus dolomieu), two important nonnative sport fishes in the system to provide information that will assist with guiding management decisions. The oldest northern pike was age 7 and the oldest smallmouth bass was age 11. Populations of both species exhibited very stable recruitment with a recruitment coefficient of determination of 0.99 for northern pike and 0.98 for smallmouth bass. Total annual mortality was estimated as 66% for northern pike and 42% for smallmouth bass. Growth of northern pike in Coeur d'Alene Lake was comparable to the 50–75th percentiles of growth exhibited by lentic northern pike populations across North America. Northern pike in Coeur d'Alene Lake were most similar to populations in the north-central and northeast United States with fast growth rates and short life spans. In contrast, smallmouth bass grew slowly and generally fell within the 5th percentile of lentic smallmouth bass populations in North America. Smallmouth bass in Coeur d'Alene Lake were similar to other populations in northern regions of the United States displaying slow growth rates with high longevity. Results of this study provide important insight on nonnative northern pike and smallmouth bass population dynamics.

  8. Swimming muscles power suction feeding in largemouth bass.

    Science.gov (United States)

    Camp, Ariel L; Roberts, Thomas J; Brainerd, Elizabeth L

    2015-07-14

    Most aquatic vertebrates use suction to capture food, relying on rapid expansion of the mouth cavity to accelerate water and food into the mouth. In ray-finned fishes, mouth expansion is both fast and forceful, and therefore requires considerable power. However, the cranial muscles of these fishes are relatively small and may not be able to produce enough power for suction expansion. The axial swimming muscles of these fishes also attach to the feeding apparatus and have the potential to generate mouth expansion. Because of their large size, these axial muscles could contribute substantial power to suction feeding. To determine whether suction feeding is powered primarily by axial muscles, we measured the power required for suction expansion in largemouth bass and compared it to the power capacities of the axial and cranial muscles. Using X-ray reconstruction of moving morphology (XROMM), we generated 3D animations of the mouth skeleton and created a dynamic digital endocast to measure the rate of mouth volume expansion. This time-resolved expansion rate was combined with intraoral pressure recordings to calculate the instantaneous power required for suction feeding. Peak expansion powers for all but the weakest strikes far exceeded the maximum power capacity of the cranial muscles. The axial muscles did not merely contribute but were the primary source of suction expansion power and generated up to 95% of peak expansion power. The recruitment of axial muscle power may have been crucial for the evolution of high-power suction feeding in ray-finned fishes.

  9. Relationship of Broodstock Weight and Hatching Yield of III. Generation (F3 Black Sea Trout (Salmo trutta labrax Pallas, 1814

    Directory of Open Access Journals (Sweden)

    Hakan Baki

    2015-07-01

    Full Text Available In this study, this was the aimed to determine the reproduction and hatching yields in different weight groups in hatchery origin rootstocks (F3 Black Sea trout (Salmo trutta labrax Pallas, 1814. In this study, the female fishes which produced from natural rootstock were used average weight 1437±134.6 g (Group I 2737±210.2 g (Group II and 3785±162.3 g (Group III. After the stripping process; according to the groups, were determined respectively eggs absolute fecundity (number of eggs/broodfish is average 2353±205, 5361±506, 6603±491, relative fecundity (number of eggs/kg is average1687±166, 1968±194, 1744±114. The egg diameter (mm 4.89±0.16, 5.28±0.07, 5.31±0.06 egg weight (mg 77±6, 90±4, 96±3 groups I., II. and III. respectively was calculated. The fertilization ratio (% 95.49±1.23, 96.49±1.14, 98.39±0.52, browse rate (% 79.97±5.36, 84.20±5.23, 94.70±1.50, has been identified. The output rate (% 60.57±7.86, 67.66±6.42, 84.83±3.09, the hatchery efficiency (% 58.14±7.95, 69.77±6.25, 83.51±3.29 was found. relative fecundity average value 168±160, 1968±194, 1744±114.The existence of relationship among the weight of the brood-stock, absolute fecundity (0.0198, 0.0012, 0.3499, and relative egg fecundity (0.3415, 0.0494, 0.00 was tested by regression analysis and consequently the difference between groups were significant. This was determined that between fish weight and absolute fecundity relationship is weak directly proportional for each group, between fish weight and relative fecundity is weak inversely proportional in Group I and II, the Group III is weak for directly proportional.

  10. The effects of bupropion on hybrid striped bass brain chemistry and predatory behavior.

    Science.gov (United States)

    Sweet, Lauren E; Bisesi, Joseph H; Lei, E N Y; Lam, Michael H W; Klaine, Stephen J

    2016-08-01

    Increased use of antidepressants has led to an increase in their detection in final treated wastewater effluents and receiving streams. Antidepressants are intended to modify human behavior by altering brain chemistry, and because of the high functional conservation of antidepressant target receptors in vertebrates, aquatic organisms may be at risk. The antidepressant bupropion is designed to alter brain norepinephrine and dopamine concentrations in humans. The objective of the present study was to understand if alteration of dopaminergic neurotransmitter concentrations in the hybrid striped bass (Morone saxatilis × Morone chrysops) brain by bupropion would alter this predator's ability to capture prey. The authors exposed hybrid striped bass to bupropion in a static system for 6 d, followed by a 6-d recovery period. During the present study's 12-d experiment, each hybrid striped bass was fed 4 unexposed fathead minnows every 3 d, and the time it took the hybrid striped bass to consume each of those 4 fathead minnows was quantified. After each feeding event, hybrid striped bass brains were harvested and analyzed for changes in several brain neurotransmitter concentrations, including serotonin, norepinephrine, dopamine, and many of their metabolites. Although bupropion altered the concentration of dopamine and many of the dopaminergic neurotransmitter metabolite concentrations in the brains on day 3 of the exposure, it did not alter the time to capture prey. This suggests that alteration of dopaminergic neurotransmitter concentrations in the hybrid striped bass brain does not alter a predator's ability to capture prey. Environ Toxicol Chem 2016;35:2058-2065. © 2016 SETAC. PMID:26748934

  11. Bass-Ihara Zeta functions for non-uniform tree lattices and class number asymptotics

    OpenAIRE

    Deitmar, Antonius; Kang, Ming-Hsuan

    2014-01-01

    It is shown that the Euler product giving the Bass-Ihara zeta function of a finite graph also converges in the case of a non-compact arith- metic quotient graph. Despite the infinite-dimensional setting, it turns out to be a rational function, generally with zeros and poles, in contrast to the compact case. The determinant formulas of Bass and Ihara hold true if one defines the determinant as limit of all finite principal minors. From this analysis, a prime geodesic theorem is derived, which,...

  12. Growth, dispersal, mortality, and contribution of largemouth bass stocked into Chickamauga Lake, Tennessee

    Science.gov (United States)

    Hoffman, K.J.; Bettoli, P.W.

    2005-01-01

    Marked fingerling largemouth bass Micropterus salmoides (both northern M. s. salmoides and Florida subspecies M. s. floridanus and their hybrid) were stocked into Chickamauga Lake, Tennessee, to enhance angling and introgress the Florida subspecies into the local gene pool. We evaluated mass marking and stocking success by sampling the stocked fish for 1 year poststocking. More than 128,000 fingerlings (35-64 mm total length) were immersed in a solution of 500 mg/L oxytetracycline (OTC) for 6 h and stocked into four embayments in the lake in spring 2002; two additional embayments served as controls and were not stocked (these embayments contained only wild, indigenous fish). In a blind test, 97% of sagittal otoliths were correctly scored as marked or unmarked. In a subsequent test, the OTC marks were clearly visible on every otolith removed from 240 OTC-treated bass held for 30 d. Age-0 largemouth bass were sampled with DC electrofishing gear at 7-19, 44-61, and 119-139 d after stocking, and sampling was conducted along 100-m transects within 1 km of the stocking sites in each embayment. Of all recaptures in the first sample, 31% occurred more than 600 m from the nearest stocking site, indicating rapid dispersal by some fish. Survival of stocked and wild age-0 largemouth bass was similar and low (4.5-6.9%) in two embayments; in the other two embayments, stocked fish survived at lower rates (0-4.3%) than wild fish (33.7-49.9%). Mean catches of all age-0 largemouth bass in the first sample were positively related to the number of fish stocked. By October 2002, the mean catch of all age-0 largemouth bass was similar among embayments. Contribution of stocked fish declined to approximately 2% (2 of 91 fish) the following spring. Cost per fingerling increased from US$0.35 at stocking to $12.00 at 140 d poststocking. Increasing the abundance of largemouth bass was not the primary objective of this stocking effort, but stocked fish will have to survive much better if

  13. Swimming muscles power suction feeding in largemouth bass.

    Science.gov (United States)

    Camp, Ariel L; Roberts, Thomas J; Brainerd, Elizabeth L

    2015-07-14

    Most aquatic vertebrates use suction to capture food, relying on rapid expansion of the mouth cavity to accelerate water and food into the mouth. In ray-finned fishes, mouth expansion is both fast and forceful, and therefore requires considerable power. However, the cranial muscles of these fishes are relatively small and may not be able to produce enough power for suction expansion. The axial swimming muscles of these fishes also attach to the feeding apparatus and have the potential to generate mouth expansion. Because of their large size, these axial muscles could contribute substantial power to suction feeding. To determine whether suction feeding is powered primarily by axial muscles, we measured the power required for suction expansion in largemouth bass and compared it to the power capacities of the axial and cranial muscles. Using X-ray reconstruction of moving morphology (XROMM), we generated 3D animations of the mouth skeleton and created a dynamic digital endocast to measure the rate of mouth volume expansion. This time-resolved expansion rate was combined with intraoral pressure recordings to calculate the instantaneous power required for suction feeding. Peak expansion powers for all but the weakest strikes far exceeded the maximum power capacity of the cranial muscles. The axial muscles did not merely contribute but were the primary source of suction expansion power and generated up to 95% of peak expansion power. The recruitment of axial muscle power may have been crucial for the evolution of high-power suction feeding in ray-finned fishes. PMID:26100863

  14. Maternally transferred mercury in wild largemouth bass, Micropterus salmoides

    International Nuclear Information System (INIS)

    Maternal transfer of mercury in fish represents a potential route of elimination for adult females and a risk to developing embryos. To better quantify maternal transfer, we measured Hg in female largemouth bass (Micropterus salmoides) muscle and eggs from six waterbodies. Mercury in eggs from two waterbodies exceeded a US federal screening level (0.3 μg g−1) and was likely high enough to cause adverse reproductive effects. We found a curvilinear relationship between female and egg Hg. Fish with −1 Hg had low levels of Hg in eggs; those with Hg >0.37 μg g−1 showed a direct relationship between egg and muscle Hg (Log10 egg Hg = −1.03 + 1.18 * log10 muscle tissue Hg + 2.15 * (log10 muscle tissue Hg + 0.35)2). We also report higher maternal transfer (0.2–13.2%) and higher ratios of egg to muscle tissue Hg (4–52%) and egg to whole body Hg concentrations (7–116%) than previously observed for teleost fish. Highlights: •Previous work suggests maternal Hg transfer in teleosts is consistently low. •We provide evidence that teleosts can have high maternal Hg transfer. •Females with low Hg had similar and low concentrations of Hg in their eggs. •Females with high Hg had Hg in eggs that increased with somatic tissue Hg. •Egg Hg from high Hg females exceeded adverse effect levels. -- Capsule: Here we report higher maternal transfer and higher ratios of egg to muscle tissue Hg than previously observed for teleost fish

  15. Relations between habitat variability and population dynamics of bass in the Huron River, Michigan

    Science.gov (United States)

    Bovee, Ken D.; Newcomb, Tammy J.; Coon, Thomas G.

    1994-01-01

    One of the assumption of the Instream Flow Incremental Methodology (IFIM) is that the dynamics of fish populations are directly or indirectly related to habitat availability. Because this assumption has not been successfully tested in coolwater streams, questions arise regarding the validity of the methodology in such streams. The purpose of our study was to determine whether relations existed between habitat availability and population dynamics of smallmouth bass (Micropterus dolomieu) and rock bass (Ambloplites rupestris) in a 16-km reach of the Huron River in southeastern Michigan. Both species exhibited strong to moderate carryover of year classes from age 0 through age 2, indicating that adult populations were related to factors affecting recruitment. Year-class strength and subsequent numbers of yearling bass were related to the availability of young-of-year habitat during the first growing season for a cohort. Number of age-0, age-1, and adult smallmouth bass were related to the average length at age 0 for the cohort. Length at age 0 was associated with young-of-year habitat and thermal regime during the first growing season. Rock bass populations exhibited similar associations among age classes and habitat variables. Compared to smallmouth bass, the number of age-2 rock bass was associated more closely with their length at age 0 than with year-class strength. Length at age 0 and year-class strength of rock bass were associated with the same habitat variables as those related to age-0 smallmouth bass. We hypothesize that an energetic mechanism linked thermal regime to length at age 0 and that increased growth resulted in higher survival rates from age 0 to age 1. We also postulate that young-of-year habitat provided protection from predators, higher production of food resources, and increased foraging efficiency. We conclude that the IFIM is a valid methodology for instream flow investigations of coolwater streams. The results for our study support the

  16. Laboratory efficacy of florfenicol against Streptococcus iniae infection in sunshine bass

    Science.gov (United States)

    An experimental feeding trial was performed to evaluate the efficacy of florfenicol (FF) in controlling Streptococcus iniae infection in sunshine bass (SB). Doses of FF tested were 0, 5, 10, 15 and 30 mg active ingredient per kilogram of fish body weight (BW) per day. Administration of medicated f...

  17. 77 FR 76942 - 2013-2014 Summer Flounder and Scup Specifications; 2013 Black Sea Bass Specifications...

    Science.gov (United States)

    2012-12-31

    ... 2013 ABC for black sea bass is the status quo ABC of 4.50 million lb (2,041 mt), and the 2013 ACTs... Management Plan, as well as to ensure compliance with the Magnuson-Stevens Fishery Conservation and Management Act. The intent of this action is to establish harvest levels and other management measures...

  18. Low frequency sound reproduction in irregular rooms using CABS (Control Acoustic Bass System)

    DEFF Research Database (Denmark)

    Celestinos, Adrian; Nielsen, Sofus Birkedal

    2011-01-01

    Early investigations on low frequency sound reproduction in rectangular rooms using CABS (Controlled Acoustic Bass System) have shown good results on simulations and measurements in real rooms. CABS takes the advantage of having a rectangular room with parallel walls. By using two low frequency l...

  19. Effect of salinity on expression of branchial ion transporters in striped bass (Morone saxatilis)

    DEFF Research Database (Denmark)

    Tipsmark, Christian Kølbaek; Madsen, Steffen Søndergaard; Borski, Russell John

    2004-01-01

    The time course of osmoregulatory adjustments and expressional changes of three key ion transporters in the gill were investigated in the striped bass during salinity acclimations. In three experiments, fish were transferred from fresh water (FW) to seawater (SW), from SW to FW, and from 15-ppt...

  20. Use of non-natal estuaries by migratory striped bass (Morone saxatilis) in summer

    Science.gov (United States)

    Mather, M. E.; Finn, John T.; Ferry, K.H.; Deegan, Linda A.; Nelson, G.A.

    2009-01-01

    For most migratory fish, little is known about the location and size of foraging areas or how long individuals remain in foraging areas, even though these attributes may affect their growth, survival, and impact on local prey. We tested whether striped bass (Morone saxatilis Walbaum), found in Massachusetts in summer, were migratory, how long they stayed in non-natal estuaries, whether observed spatial patterns differed from random model predictions, whether fish returned to the same area across multiple years, and whether fishing effort could explain recapture patterns. Anchor tags were attached to striped bass that were caught and released in Massachusetts in 1999 and 2000, and recaptured between 1999 and 2007. In fall, tagged striped bass were caught south of where they were released in summer, confirming that fish were coastal migrants. In the first summer, 77% and 100% of the recaptured fish in the Great Marsh and along the Massachusetts coast, respectively, were caught in the same place where they were released. About two thirds of all fish recaptured near where they were released were caught 2-7 years after tagging. Our study shows that smaller (400-500 mm total length) striped bass migrate hundreds of kilometers along the Atlantic Ocean coast, cease their mobile lifestyle in summer when they use a relatively localized area for foraging (<20 km2), and return to these same foraging areas in subsequent years.

  1. Indigenous development and performance evaluation of BARC aerodynamic size separator (BASS)

    CERN Document Server

    Singh, S; Khan, A; Mayya, Y S; Narayanan, K P; Purwar, R C; Sapra, B K; Sunny, F

    2002-01-01

    Commercially available cascade impactors, commonly used for aerodynamic size separation of aerosol particles, are based on the principle of inertial impaction. As of now, these instruments are imported at a cost of several lakhs of rupees; hence an effort has been made to develop an aerodynamic particle sizer indigenously in BARC. This unit, referred to as BARC Aerodynamic Size Separator (BASS), separates aerosols into seven size classes ranging from 0.53 mu m to 10 mu m and operates at a flow rate of 45 Ipm. Intercomparison studies between the standard Andersen Mark-II (Grasbey Andersen Inc.) impactor and BASS using nebulizer generated aerosols have consistently shown excellent performance by BASS in all respects. In particular, BASS yielded the parameters of polydisperse aerosols quite accurately. Experiments to evaluate the individual stage cut-off diameters show that these are within 8% of their designed value for all stages except the higher two stages which indicate about 30% lower values than the desig...

  2. Comparison of tank treatments with copper sulfate and potassium permanganate for sunshine bass with ichthyobodosis

    Science.gov (United States)

    The biflagellated single-cell parasite Ichthyobodo nectator can cause significant losses among fish populations, particularly those cultured in tanks. Potassium permanganate and CuSO4 treatments were evaluated against a naturally-occurring I. nectator infestation on sunshine bass raised in tanks. F...

  3. Phytoplankton succession in sunshine bass fry ponds and the effect of Aquashade®

    Science.gov (United States)

    Phytoplankton management in aquaculture ponds is very critical in maintaining good quality water for culturing fish especially during the fry and fingerling stages. Though much is known about succession in catfish ponds, that is not the case for sunshine bass ponds. This study was designed to look a...

  4. Amen-break : Från ett samplat trumbreak till nya trumkomp i drum'n'bass-musik.

    OpenAIRE

    Björni, Patrik

    2012-01-01

    Drum’n’bass är en genre inom elektronisk dansmusik som utvecklades under 1990-talet. Genren kännetecknas av rytmiskt komplexa trumkomp. Syftet med detta arbete är att ge en uppfattning av trumkomp, vilka kallas för breakbeats i drum’n’bass-musik. Med hjälp av litteratur har jag forskat i historien om drum’n’bass för att ge en uppfattning om hur genren utvecklades och dess bakgrund. Breakbeats är skapade genom att sampla och bearbeta trumkomp från äldre funk-och soullåtar med elektronisk...

  5. Effects of salinity on striped bass eggs and larvae from the Savannah River, Georgia

    Science.gov (United States)

    Winger, P.V.; Lasier, P.J.

    1994-01-01

    Operation of a tide gate installed in the Savannah River by the U.S. Army Corps of Engineers to reduce dredging activities increased salinities upstream in important spawning habitat for striped bass Morone saxatilis. To assess the effects of salinity on survival and growth of striped bass at early life stages, newly fertilized eggs and 48-h-posthatch were exposed to serial dilutions of seawater, with salinities ranging from 0 to 33 permill (g/L) in increments of 3 permill in addition, older larvae (5-d posthatch) were exposed to salinities of 0, 6, 12, 18, and 24 permill. Eggs were exposed until 24 h posthatch, 48-h-posthatch larvae were exposed for 10 d, and 5-d-posthatch larvae were exposed for 6 d. Eggs died within 24 h in salinities greater than 18 permill. Both survival and total length of larvae hatched from eggs exposed to salinities of 15 permill or higher were reduced. Percent mortality and mean total lengths of newly hatched larvae followed the same pattern for each of three sets of salinity regimes (i.e., changes in salinities over time) that striped bass eggs might encounter during passage downstream in the Savannah River. Hardening eggs in freshwater did not increase survival or length of hatched larvae over that shown by eggs hardened in saline water. The 5-d-posthatch larvae were less sensitive to salinity than the 48-h-posthatch larvae. Survival of larvae was negatively con-elated with both salinity and exposure time. For 48-h-posthatch larvae, the 10-d LC50 (the salinity lethal to 50% of the test fish within 10 d) was 10 permill. Probabilities of survival for larval striped bass exposed to different salinities for different amounts of time can be estimated from curves generated from models of survival analysis. Salinities judged to be critical to Savannah River striped bass eggs and larvae are those greater than 9 permill.

  6. Mercury and selenium concentrations in largemouth bass and other fishes of the Lower Suwannee National Wildlife Refuge

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — From March 22 to April 7, 1990, 71 largemouth bass (Micropterus salmoides) and four other fish species (total of seven fish) were collected from ten locations on or...

  7. COMPARISON OF THE REPRODUCTIVE PHYSIOLOGY OF LARGEMOUTH BASS, MICROPTERUS SALMOIDES, COLLECTED FROM THE ESCAMBIA AND BLACKWATER RIVERS IN FLORIDA

    Science.gov (United States)

    Largemouth bass (LMB), Micropterus salmoides, were taken from the Escambia River (contaminated site) and the Blackwater River (reference site) near Pensacola, Florida. The Escambia River collection occurred downstream of the effluent from two identified point sources of pollution...

  8. Metal contaminants in Largemouth bass (Micropterus salmoides) collected in large dams from Tejo River basin and small irrigation dams

    OpenAIRE

    L.P. Andrade; Antunes, P.; Paulo, L; Pereira, M. E.; A.M. Rodrigues

    2012-01-01

    Largemouth bass (Micropterus salmoides) is a very important fresh water fish in the Portuguese regional cuisine mainly in the countryside (Central region and north Alentejo). Because there’s no aquaculture industry, all eaten largemouth bass in Portugal are collected in large dams (Basins of Tejo and Guadiana rivers) and small irrigation dams. For decades, the Tejo River received environmental pollutants from non-point and point sources that included intensive agriculture, industrial entities...

  9. Effect of stocking density on growth and survival of spotted sand bass Paralabrax maculatofasciatus larvae in a closed recirculating system

    OpenAIRE

    Álvarez González, Carlos; Ortiz Galindo, José Luis; Dumas, Silvie; Martínez Díaz, Sergio Francisco; Hernández Ceballos, Dora Esther; Grayeb del Alamo, Tanos; Moreno Lagorreta, Manuel; Peña Martínez, Renato; Civera Cerecedo, Roberto

    2001-01-01

    The effect on growth and survival of the initial stocking density (50, 100, 150, and 200 larvae/ L) in larval rearing of spotted sand bass was evaluated over 30 d in a closed recirculating system. Larvae were fed with rotifers, copepods, nauplii and adult Artemia, and spotted sand bass yolk-sac larvae. Water quality was monitored daily. The notochordal or standard length of sampled larvae was measured by image analysis. Specific growth rates at each density were compared by covariance analysi...

  10. MODELISATION, CONCEPTION ET COMMANDE DE GENERATRICES A RELUCTANCE VARIABLE BASSE VITESSE

    OpenAIRE

    Moreau, Luc

    2005-01-01

    Les travaux présentés portent sur la conception et la commande d'un ensemble convertisseur-génératrice basse vitesse pour les applications éoliennes. Une génératrice 10kW, 50 tr/min (contrainte typique d'une éolienne autonome) est étudiée. La machine à réluctance variable (MRV) à plots dentés et à grand nombre de dents est retenue car elle est bien adaptée à un fonctionnement à basse vitesse de rotation permettant la simplification ou la suppression du multiplicateur de vitesse utilisé avec l...

  11. Interplay Between Amphioxus Complement with Sea Bass Macrophages: Opsonic Activity of Amphioxus Humoral Fluids

    Institute of Scientific and Technical Information of China (English)

    PAN Junli; LIU Min; ZHANG Shicui

    2011-01-01

    Previous studies have shown the existence of a complement system in the amphioxus Branchiostoma japonicum.However,whether it has an opsonic activity similar to that of vertebrates remains unknown.We demonstrated that the humoral fluid (HF)of amphioxus promoted the phagocytosis of yeast cells with sea bass (Lateolabraxjaponicus) macrophages,whereas the C3-depleted and heated HF significantly lost the phagocytosis-promoting capacity.In addition,the precipitation of factor B (Bf) led to a marked loss of opsonic activity.Moreover,C3 fragments in the HF were found to bind to yeast cell surfaces.The results indicate that the amphioxus complement system is an important element involved in the opsonic activity,which promotes the sea bass macrophage phagocytosis by tagging yeast cells with C3 fragments via the activation of alternative complement pathway.

  12. Largemouth bass (Micropterus salmoides Lacépède: results of farming trials

    Directory of Open Access Journals (Sweden)

    R. Vicenzi

    2010-01-01

    Full Text Available In the last years in the United States, the demand of largemouth bass (Micropterus salmoides larger than the size usually produced for sport fishing purposes has increased. The growth phase is carried on in ponds, after having evaluated different strains (Williamson and Carmichael, 1990 using feeds for salmonids (Tidwell et al., 1996 supplemented with amino acids (Coyle et al., 2000. Also in Italy, the market currently requires largemouth bass of 300-500 g. This situation has induced one farmer to cooperate with our research centre and carried out trials to verify the possibility of rearing this fish, from the fingerling stage up to the size suitable for food, using the same farming techniques as those currently being applied in the United States (Davis and Lock, 1997.

  13. BASS 4: a software system for ergonomic design and evaluation of working hours

    Directory of Open Access Journals (Sweden)

    Carsten Schomann

    2004-12-01

    Full Text Available OBJECTIVE: To extend an existing computer programme for the evaluation and design of shift schedules (BASS 3 by integrating workload as well as economic aspects. METHODS: The redesigned prototype BASS 4 includes a new module with a suitable and easily applicable screening method (EBA for the assessment of the intensity of physical, emotional and cognitive workload components and their temporal patterns. Specified criterion functions based on these ratings allow for an adjustment of shift and rest duration according to the intensity of physical and mental workload. Furthermore, with regard to interactive effects both workload and temporal conditions, e.g. time of day, are taken into account. In a second new module, important economic aspects and criteria have been implemented. Different ergonomic solutions for scheduling problems can now also be evaluated with regard to their economic costs. RESULTS: The new version of the computer programme (BASS 4 can now simultaneously take into account numerous ergonomic, legal, agreed and economic criteria for the design and evaluation of working hours. CONCLUSIONS: BASS 4 can now be used as an instrument for the design and the evaluation of working hours with regard to legal, ergonomic and economic aspects at the shop floor as well as in administrative (e.g. health and safety inspection and research problems.OBJETIVOS: Expandir um programa computacional existente para planejamento e avaliação dos horários de turnos (BASS 3 por meio da incorporação da carga de trabalho e características econômicas. MÉTODOS: O protótipo BASS 4 contém um novo módulo com um método de triagem (EBA conveniente e de fácil aplicação para a avaliação da intensidade dos componentes físico, emocional e cognitivo da carga de trabalho e seus padrões temporais. O uso de critérios específicos com base nestas avaliações possibilita ajustar a duração do turno e do descanso de acordo com a intensidade da carga de

  14. Water chemistry characterization and component performance of a recirculating aquaculture system producing hybrid striped bass

    OpenAIRE

    Easter, Christopher

    1992-01-01

    Eight identical and independent pilot scale recirculating aquaculture production systems were populated with fingerling hybrid striped bass (Morone chrysops female x Morone saxatilis male). Three population densities were established with two replicates at 132 fishlm3 and three replicates each at 66 and 33 fishlm3. Water chemistry and water quality characteristics were monitored throughout the 228 day growth trial for all eight systems. A system component performance analys...

  15. Application of a bioenergetics model for hatchery production: Largemouth bass fed commercial diets

    Science.gov (United States)

    Csargo, Isak J.; Michael L. Brown; Chipps, Steven R.

    2012-01-01

    Fish bioenergetics models based on natural prey items have been widely used to address research and management questions. However, few attempts have been made to evaluate and apply bioenergetics models to hatchery-reared fish receiving commercial feeds that contain substantially higher energy densities than natural prey. In this study, we evaluated a bioenergetics model for age-0 largemouth bass Micropterus salmoidesreared on four commercial feeds. Largemouth bass (n ≈ 3,504) were reared for 70 d at 25°C in sixteen 833-L circular tanks connected in parallel to a recirculation system. Model performance was evaluated using error components (mean, slope, and random) derived from decomposition of the mean square error obtained from regression of observed on predicted values. Mean predicted consumption was only 8.9% lower than mean observed consumption and was similar to error rates observed for largemouth bass consuming natural prey. Model evaluation showed that the 97.5% joint confidence region included the intercept of 0 (−0.43 ± 3.65) and slope of 1 (1.08 ± 0.20), which indicates the model accurately predicted consumption. Moreover model error was similar among feeds (P = 0.98), and most error was probably attributable to sampling error (unconsumed feed), underestimated predator energy densities, or consumption-dependent error, which is common in bioenergetics models. This bioenergetics model could provide a valuable tool in hatchery production of largemouth bass. Furthermore, we believe that bioenergetics modeling could be useful in aquaculture production, particularly for species lacking historical hatchery constants or conventional growth models.

  16. A simple model for predicting survival of angler-caught and released largemouth bass

    Science.gov (United States)

    Wilde, G.R.; Pope, K.L.

    2008-01-01

    We conducted a controlled experiment in the laboratory to assess the influence of anatomical hooking location and water temperature on survival of angler-caught and released largemouth bass Micropterus salmoides. Survival was 98% (58 of 59 fish) among fish that were hand-hooked within the oral cavity (including the gills), whereas survival was 66% (33 of 50 fish) among fish that were hand-hooked in the esophagus. Survival of hooked fish was not significantly influenced by water temperature (7-27??C) or the hooking location X water temperature interaction. We combined our results with prior research to develop a predictive model of largemouth bass survival, which was 98.3% (SD = 1.87%) for fish hooked in the oral cavity and 55.0% (SD = 9.70%) for fish hooked in the esophagus. The model is valid for water temperatures ranging from 7??C to 27??C and allows one to estimate, with known precision, the survival of angler-caught and released largemouth bass without the need for controlled studies or for holding fish in pens or cages to assess delayed mortality. ?? Copyright by the American Fisheries Society 2008.

  17. Summer habitat selection by striped bass, Morone Saxatilis, in Cherokee Reservoir, Tennessee, 1977

    Energy Technology Data Exchange (ETDEWEB)

    Waddle, H.R.; Coutant, C.C.; Wilson, J.L.

    1980-02-01

    Summer habitat selection patterns of 18 adult striped bass (Morone saxatilis) in Cherokee Reservoir were monitored with externally attached temperature-sensing acoustic or radio transmitters from June through September 1977. Mortalities of adult striped bass in this reservoir were hypothesized to be related to high summer temperatures and low dissolved oxygen (DO). The inhabited areas or refuges differed from noninhabited areas by maintaining temperatures less than or equal to 22 C and DO concentrations greater than 5 mg/liter. Total water hardness, pH, and water transparency were not significantly different among refuges and noninhabited areas. Movement of fish outside refuges occurred more frequently and for longer periods during June when the summer pattern of high temperatures and low DO was less severe. Fish experienced temperatures between 15 and 27 C with mean temperatures of individuals ranging from 18.5 to 22.0 C. Several tagged fish migrated outside the refuges and selected the lowest available temperature, generally near 21 C, even though DO concentrations at these temperatures were 3 mg/liter or less. Long-term survival of tagged and nontagged fish outside refuges was undetermined because no fish were tracked outside a refuge for more than 12 days without being lost. This study indicates that temperature strongly influences the behavior of striped bass and that adults of this species may have a thermal preferendum of approximately 21 C.

  18. Examination of spotted sand bass (Paralabrax maculatofasciatus pollutant bioaccumulation in San Diego Bay, San Diego, California

    Directory of Open Access Journals (Sweden)

    Chad L. Loflen

    2013-11-01

    Full Text Available The spotted sand bass (Paralabrax maculatofasciatus is an important recreational sport and subsistence food fish within San Diego Bay, a large industrialized harbor in San Diego, California. Despite this importance, few studies examining the species life history relative to pollutant tissue concentrations and the consumptive fishery exist. This study utilized data from three independent spotted sand bass studies from 1989 to 2002 to investigate PCB, DDT, and mercury tissue concentrations relative to spotted sand bass age and growth in San Diego Bay, with subsequent comparisons to published pollutant advisory levels and fishery regulations for recreational and subsistence consumption of the species. Subsequent analysis focused on examining temporal and spatial differences for different regions of San Diego Bay. Study results for growth confirmed previous work, finding the species to exhibit highly asymptotic growth, making tissue pollutant concentrations at initial take size difficult if not impossible to predict. This was corroborated by independent tissue concentration results for mercury, which found no relationship between fish size and pollutant bioaccumulation observed. However, a positive though highly variable relationship was observed between fish size and PCB tissue concentration. Despite these findings, a significant proportion of fish exhibited pollutant levels above recommended state recreational angler consumption advisory levels for PCBs and mercury, especially for fish above the minimum take size, making the necessity of at-size predictions less critical. Lastly, no difference in tissue concentration was found temporally or spatially within San Diego Bay.

  19. Bass-SIR model for diffusion of new products in social networks

    Science.gov (United States)

    Fibich, Gadi

    2016-09-01

    We consider the diffusion of new products in social networks, where consumers who adopt the product can later "recover" and stop influencing others to adopt the product. We show that the diffusion is not described by the susceptible-infected-recovered (SIR) model, but rather by a new model, the Bass-SIR model, which combines the Bass model for diffusion of new products with the SIR model for epidemics. The phase transition of consumers from nonadopters to adopters is described by a nonstandard Kolmogorov-Johnson-Mehl-Avrami model, in which clusters growth is limited by adopters' recovery. Therefore, diffusion in the Bass-SIR model only depends on the local structure of the social network, but not on the average distance between consumers. Consequently, unlike the SIR model, a small-worlds structure has a negligible effect on the diffusion. Moreover, unlike the SIR model, there is no threshold value above which the diffusion will peter out. Surprisingly, diffusion on scale-free networks is nearly identical to that on Cartesian ones.

  20. 137Cs elimination by chronically-contaminated largemouth bass (Micropterus salmoides)

    International Nuclear Information System (INIS)

    The temperature-dependent 137Cs biological half-times (Tb) of lifetime-exposed largemouth bass (Micropterus salmoides) from a nuclear cooling reservoir at the US Department of Energy's Savannah River Site were calculated from whole-body measurements of live fish and compared with literature records for acutely and chronically-contaminated fish. The Tb's of the bass averaged 322 d, 225 d, and 140 d at 15, 20, and 26 C, respectively. These mean Tb's were 1.7 to 2.5 times longer than would be expected for acutely contaminated fish, and 1.2 to 1.8 times longer than those predicted for fish at steady-state with their environment according to recent models. This slower elimination did not appear to result from slower elimination from skeletal muscle compared with other soft tissues, in that the muscle to whole-body 137Cs concentration ratios after the elimination period were similar to those of freshly-caught bass. Their results suggested that elimination rates estimated from the terminal elimination components of acutely-dosed fish may not reflect the elimination rates of fish exposed to contaminants throughout their lifetime, even when care is taken to allow sufficient time for absorption of the dose

  1. Purification and characterization of the carbonic anhydrase enzyme from Black Sea trout (Salmo trutta Labrax Coruhensis) kidney and inhibition effects of some metal ions on enzyme activity.

    Science.gov (United States)

    Kucuk, Murat; Gulcin, İlhami

    2016-06-01

    In this study, the carbonic anhydrase (CA) enzyme was purified from Black Sea trout (Salmo trutta Labrax Coruhensis) kidney with a specific activity of 603.77EU/mg and a yield of 35.5% using Sepharose-4B-l-tyrosine- sulphanilamide affinity column chromatography. For determining the enzyme purity and subunit molecular mass, sodiumdodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) was performed and single band was observed. The molecular mass of subunit was found approximately 29.71kDa. The optimum temperature, activation energy (Ea), activation enthalpy (ΔH) and Q10 values were obtained from Arrhenius plot. Km and Vmax values for p-nitrophenyl acetate of the purified enzyme were calculated from Lineweaver-Burk graphs. In addition, the inhibitory effects of different heavy metal ions (Fe(2+), Pb(2+), Co(2+), Ag(+) and Cu(2+)) on Black Sea trout kidney tissue CA enzyme activities were investigated by using esterase method under in vitro conditions. The heavy metal concentrations inhibiting 50% of enzyme activity (IC50) were obtained. Finally Ki values and inhibition types were calculated from Lineweaver-Burk graphs. PMID:27175889

  2. Abundance and Distribution of Walleye, Northern Squawfish, and Smallmouth Bass in John Day Reservoir, 1984-1985 Progress Report.

    Energy Technology Data Exchange (ETDEWEB)

    Beamesderfer, Raymond C.

    1985-12-01

    Sampling was conducted in John Day Reservoir to collect walleye, northern squawfish and smallmouth bass. Changes in distributions during sampling were characterized from changes in catch per unit effort (CPUE) in sampling areas. Observed movements of marked and radiotagged fish were examined and used to define discrete populations. Abundances were estimated using a modified Schnabel multiple mark and recapture estimator. Abundance estimates were corrected for angler harvest, size specific vulnerability to gear, recruitment due to growth and tag loss during sampling. Age composition of catch was determined to characterize relative contributions of various year classes to the populations. Ages at which fish were fully recruited to gear were defined by catch curves. Survival of fully recruited year classes was calculated from differences in CPUE's between 1984 and 1985. Mean length at age was estimated and used to determine age specific incremental growth. Eighty-eight percent of walleye were caught in McNary tailrace or Irrigon-Paterson, whereas 95% of smallmouth bass were caught from Irrigon-Paterson to the John Day forebay. Abundances of walleye and northern squawfish with fork lengths greater than 250 mm and smallmouth bass with fork lengths greater than 200 mm were estimated to be 16,219, 95,407, and 11,259. Anglers harvested an estimated 235 walleye, 2004 northern squawfish and 4383 smallmouth bass during the sampling season. Six-year-old walleye, 4-year-old northern squawfish and 3-year-old smallmouth bass were most abundant in catches. Walleye and smallmouth bass were fully recruited to sampling gear by age 3. Age at which northern squawfish were fully recruited was uncertain. Mean survival was 46.1% for walleye and 46.5% for northern squawfish. Mean smallmouth bass survival was 46.5% in the lower and 43.7% in the upper reservoir.

  3. Die faktorstruktuur van Bass se veelfaktor- leierskapsvraelys in die Suid-Afrikaanse konteks

    Directory of Open Access Journals (Sweden)

    C .P. Ackermann

    2000-06-01

    Full Text Available The factor structure of Bass's Multifactor Leadership Questionnaire in the South African context. The aim of the study was to determine whether the factor structure of Bass's Multifactor Leadership Questionnaire (MLQ, as a measure of transformational leadership, could be replicated within the South African context. The MLQ was chosen not only because it promised to be a valid and reliable measuring instrument of the construct in question, but also due to the fact that there was an urgent need for such an instrument in the management of human resources within organisations undergoing transformation. The MLQ was administered to 406 subjects within the military context and was subjected to factor analysis and item analysis. The factor analysis yielded three factors, namely transformational leadership, transactional leadership and avoidance of leadership ("laissez faire" leadership. The reliabilities of the scales were determined by means of Cronbach's coefficient alpha, and yielded coefficients of 0,944, 0,736 and 0,803 respectively. The factor structure as conceptualised by Bass (1985 was largely confirmed in the present study. Opsomming Die doel van die studie was om vas te stel of die faktorstruktuur van die Veelfaktorleierskapsvraelys (MLQ van Bass, as maatstafvan transformasionele leierskap, in die Suid-Afrikaanse konteks gerepliseer kon word. Die MLQ is gekies omdat dit belofte inhou as n geldige en betroubare meetinstrument van die onderhawige konstruk, en ook weens die feit dat daar 'n dringende behoefte bestaan aan so n instrument vir gebruik in die bestuur van menslike hulpbronne in organisasies tydens verandering. Die MLQ is op 406 proefpersone binne militêre konteks toegepas, en aan n faktorontleding en n itemontleding onderwerp. Die faktorontleding het drie faktore opgelewer, te wete transformasionele leierskap, transaksionele leierskap en vermyding van leierskap ("laissez faire"-leierskap. Die betroubaarheid van die skale is bepaal

  4. Continuous Tidal Streamflow and Gage-Height Data for Bass and Cinder Creeks on Kiawah Island, South Carolina, September 2007

    Science.gov (United States)

    Conrads, Paul A.; Erbland, John W.

    2009-01-01

    A three-dimensional model of Bass and Cinder Creeks on Kiawah Island, South Carolina, was developed to evaluate methodologies for determining fecal coliform total maximum daily loads for shellfish waters. To calibrate the model, two index-velocity sites on the creeks were instrumented with continuous acoustic velocity meters and water-level sensors to compute a 21-day continuous record of tidal streamflows. In addition to monitoring tidal cycles, streamflow measurements were made at the index-velocity sites, and tidal-cycle streamflow measurements were made at the mouth of Bass Creek and on the Stono River to characterize the streamflow dynamics near the ocean boundary of the three-dimensional model at the beginning, September 6, 2007, and end, September 26, 2007, of the index-velocity meter deployment. The maximum floodtide and ebbtide measured on the Stono River by the mouth of Bass Creek for the two measurements were -155,000 and 170,000 cubic feet per second (ft3/s). At the mouth of Bass Creek, the maximum floodtide and ebbtide measurements during the 2 measurement days were +/-10,200 ft3/s. Tidal streamflows for the 21-day deployment on Bass Creek ranged from -2,510 ft3/s for an incoming tide to 4,360 ft3/s for an outgoing tide. On Cinder Creek, the incoming and outgoing tide varied from -2,180 to 2,400 ft3/s during the same period.

  5. Changes in concentrations of selenium and mercury in largemouth bass following elimination of fly ash discharge to a quarry.

    Science.gov (United States)

    Southworth, G R; Peterson, M J; Turner, R R

    1994-07-01

    Elimination of slurried fly ash discharges to a water-filled quarry was followed by a steady increase in concentrations of mercury in the axial muscle of resident largemouth bass (Micropterus salmoides). Average mercury concentrations in bass (adjusted for covariance with fish weight) increased from 0.02 micrograms/g to 0.17 micrograms/g in three years. Aqueous selenium concentrations in the quarry decreased from 25 micrograms/L to < 2 micrograms/L after elimination of fly ash discharges, but selenium concentrations in bass remained about three times background levels. Previous studies have shown selenium addition to be a viable means of ameliorating mercury contamination in fish in low alkalinity, low pH waters of northern Europe and Canada. These results suggest that selenium may also be effective at blocking the accumulation of methylmercury in harder, more alkaline waters.

  6. Zooplankton variability and larval striped bass foraging: Evaluating potential match/mismatch regulation

    Science.gov (United States)

    Chick, J.H.; Van Den Avyle, M.J.

    1999-01-01

    We quantified temporal and spatial variability of zooplankton in three potential nursery sites (river, transition zone, lake) for larval striped bass (Morone saxatilis) in Lake Marion, South Carolina, during April and May 1993-1995. In two of three years, microzooplankton (rotifers and copepod nauplii) density was significantly greater in the lake site than in the river or transition zone. Macrozooplankton (>200 ??m) composition varied among the three sites in all years with adult copepods and cladocerans dominant at the lake, and juvenile Corbicula fluminea dominant at the river and transition zone. Laboratory feeding experiments, simulating both among-site (site treatments) and within-site (density treatments) variability, were conducted in 1995 to quantify the effects of the observed zooplankton variability on foraging success of larval striped bass. A greater proportion of larvae fed in the lake than in the river or transition-zone treatments across all density treatments: mean (x), 10x and 100x. Larvae also ingested significantly more dry mass of prey in the lake treatment in both the mean and 10x density treatments. Field zooplankton and laboratory feeding data suggest that both spatial and temporal variability of zooplankton influence larval striped bass foraging. Prey density levels that supported successful foraging in our feeding experiments occurred in the lake during late April and May in 1994 and 1995 but were never observed in the river or transition zone. Because the rivers flowing into Lake Marion are regulated, it may be possible to devise flow management schemes that facilitate larval transport to the lake and thereby increase the proportion of larvae matched to suitable prey resources.

  7. Radiometric analysis of farmed fish (sea bass, gilthead bream, and rainbow trout) from Tenerife Island, Spain.

    Science.gov (United States)

    Jalili, A; López-Pérez, M; Karlsson, L; Hernández, F; Rubio, C; Hernández-Armas, J; Hardisson, A

    2009-09-01

    This study analyzed the content of gamma-emitting radionuclides in fish farmed on the island of Tenerife (Canary Islands, Spain). The fish species included in this study were sea bass, gilthead bream, and rainbow trout. The first two species are produced in offshore enclosures, while the third is produced in a freshwater fish farm. All measurements were performed using two high-purity germanium gamma-ray detectors. The content of gamma-emitting radionuclides in the fodder used to feed the different species of farmed fish studied was also determined. The following nuclides were often detected in the analyzed samples: 137Cs, 40K, 235U, 228Ac, 214Bi, 208Tl, 212Pb, and 214Pb. As a complement to this analysis, 210Po concentrations in two fish samples were determined by alpha spectrometry. The nuclide presenting the highest concentration was, as expected, the naturally occurring 40K, with an average concentration of 0.13 +/- 0.01 Bq/g (wet weight) (Bq/gww) in gilthead bream and sea bass and 0.12 +/- 0.01 Bq/gww in rainbow trout. The 235U concentrations determined in the same fish species were 0.6 +/- 0.5, 0.8 +/- 0.7, and 1.6 +/- 1.0 mBq/gww, respectively. This nuclide is seldom reported in fish samples. The concentrations of 137Cs (the only artificial nuclide determined in this study) in gilthead bream and sea bass were 0.026 +/- 0.006 and 0.044 +/- 0.01 mBq/gww, respectively. In addition to the radiometric analysis, the contribution of the analyzed nuclides to the effective dose from the mean daily intake of the fish was calculated. The calculated contribution, in terms of dose per person, produced by intake of the analyzed fish was 0.8 microSv/year. This value does not represent a significant risk to the local population.

  8. A large volume striped bass egg incubation chamber: design and comparison with a traditional method

    Science.gov (United States)

    Harper, C.J.

    2009-01-01

    I conducted a comparative study of a new jar design (experimental chamber) with a standard egg incubation vessel (McDonald jar). Experimental chambers measured 0.4 m in diameter by 1.3 m in height and had a volume of 200 L. McDonald hatching jars measured 16 cm in diameter by 45 cm in height and had a volume of 6 L. Post-hatch survival was estimated at 48, 96 and 144 h. Stocking rates resulted in an average egg density of 21.9 eggs ml-1 (range = 21.6 – 22.1) for McDonald jars and 10.9 eggs ml-1 (range = 7.0 – 16.8) for experimental chambers. I was unable to detect an effect of container type on survival to 48, 96 or 144 h. At 144 h striped bass fry survival averaged 37.3% for McDonald jars and 34.2% for experimental chambers. Survival among replicates was significantly different. Survival of striped bass significantly decreased between 96 and 144 h. Mean survival among replicates ranged from 12.4 to 57.3%. I was unable to detect an effect of initial stocking density on survival. Experimental jars allow for incubation of a larger number of eggs in a much smaller space. As hatchery production is often limited by space or water supply, experimental chambers offer an alternative to extending spawning activities, thereby reducing manpower and cost. However, the increase in the number of eggs per rearing container does increase the risk associated with catastrophic loss of a production unit. I conclude the experimental chamber is suitable for striped bass egg incubation.

  9. Diversity of estuarine movements of striped bass (Morone saxatilis): a synoptic examination of an estuarine system in southern New Jersey

    OpenAIRE

    Kenneth W. Able; Grothues, Thomas M.

    2007-01-01

    We determined the dis-tribution of multiple (n=68; 508−978 mm total length [TL]) striped bass (Morone saxatilis) along the estua-rine salinity gradient in the Mullica River−Great Bay in southern New Jersey over two years to determine the diversity of habitat use and the movements of striped bass. Ultrasoni-cally tagged fish were detected in this estuarine area by means of wireless hydrophones deployed at four gates inside the entrance of the study area and farther up to tidal freshwater (38 k...

  10. IGF-I and branchial IGF receptor expression and localization during salinity acclimation in striped bass

    DEFF Research Database (Denmark)

    Tipsmark, Christian Kølbaek; Luckenbach, John Adam; Madsen, Steffen;

    2007-01-01

    The initial response of the IGF-I system and the expression and cellular localization of IGF type-I receptor (IGF-IR) were studied in the gill of a euryhaline teleost during salinity acclimation. Exposure of striped bass (Morone saxatilis) to hyperosmotic and hypoosmotic challenges induced small...... in either plasma IGF-I, liver, or gill IGF-I mRNA, or gill IGF-IR mRNA levels. In a separate experiment, FW-acclimated fish were injected with saline or IGF-I prior to a 24-h SW challenge. Rapid regain of osmotic balance following SW transfer was hindered by IGF-I. Immunohistochemistry revealed...

  11. Dicty_cDB: Contig-U15469-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available janus argentimaculatus cathepsi... 117 8e-25 EF530141_1( EF530141 |pid:none) Actini...1( FJ807676 |pid:none) Dicentrarchus labrax cathepsin L m... 117 8e-25 FJ772427_1( FJ772427 |pid:none) Lut

  12. The Bass diffusion model on networks with correlations and inhomogeneous advertising

    Science.gov (United States)

    Bertotti, M. L.; Brunner, J.; Modanese, G.

    2016-09-01

    The Bass model, which is an effective forecasting tool for innovation diffusion based on large collections of empirical data, assumes an homogeneous diffusion process. We introduce a network structure into this model and we investigate numerically the dynamics in the case of networks with link density $P(k)=c/k^\\gamma$, where $k=1, \\ldots , N$. The resulting curve of the total adoptions in time is qualitatively similar to the homogeneous Bass curve corresponding to a case with the same average number of connections. The peak of the adoptions, however, tends to occur earlier, particularly when $\\gamma$ and $N$ are large (i.e., when there are few hubs with a large maximum number of connections). Most interestingly, the adoption curve of the hubs anticipates the total adoption curve in a predictable way, with peak times which can be, for instance when $N=100$, between 10% and 60% of the total adoptions peak. This may allow to monitor the hubs for forecasting purposes. We also consider the case of networks with assortative and disassortative correlations and a case of inhomogeneous advertising where the publicity terms are "targeted" on the hubs while maintaining their total cost constant.

  13. Methoxychlor affects multiple hormone signaling pathways in the largemouth bass (Micropterus salmoides) liver.

    Science.gov (United States)

    Martyniuk, Christopher J; Spade, Daniel J; Blum, Jason L; Kroll, Kevin J; Denslow, Nancy D

    2011-02-01

    Methoxychlor (MXC) is an organochlorine pesticide that has been shown to have estrogenic activity by activating estrogen receptors and inducing vitellogenin production in male fish. Previous studies report that exposure to MXC induces changes in mRNA abundance of reproductive genes in the liver and testes of largemouth bass (Micropterus salmoides). The objective of the present study was to better characterize the mode of action of MXC by measuring the global transcriptomic response in the male largemouth liver using an oligonucleotide microarray. Microarray analysis identified highly significant changes in the expression of 37 transcripts (p<0.001) (20 induced and 17 decreased) in the liver after MXC injection and a total of 900 expression changes (p<0.05) in transcripts with high homology to known genes. Largemouth bass estrogen receptor alpha (esr1) and androgen receptor (ar) were among the transcripts that were increased in the liver after MXC treatment. Functional enrichment analysis identified the molecular functions of steroid binding and androgen receptor activity as well as steroid hormone receptor activity as being significantly over-represented gene ontology terms. Pathway analysis identified c-fos signaling as being putatively affected through both estrogen and androgen signaling. This study provides evidence that MXC elicits transcriptional effects through the estrogen receptor as well as androgen receptor-mediated pathways in the liver.

  14. Competitive interactions between walleye (Sander vitreus) and smallmouth bass (Micropterus dolomieu) under various controlled conditions

    Science.gov (United States)

    Wuellner, M.R.; Graeb, B.D.S.; Willis, D.W.; Galster, B.J.; Selch, T.M.; Chipps, S.R.

    2011-01-01

    The range of smallmouth bass (Micropterus dolomieu) is expanding northward, creating new interactions with native predators, including walleye (Sander vitreus). We used a series of experiments to investigate competition between walleye (WAE) and smallmouth bass (SMB) at different life stages and light conditions, identified behaviors that allowed one fish to outcompete another, and evaluated whether prey switching mitigated competitive interactions. Juvenile and adult SMB appeared to outcompete WAE when fed during the daytime; neither species dominated when fed near dusk. Attack rates and capture efficiencies of both species were similar with an intra- or interspecific competitor, but SMB often exploited prey before the competitor had a chance to feed (exploitative competition) or displayed agonistic behaviors toward a potential competitor (interference competition). Prey selectivity of WAE or SMB did not differ when by themselves or with a potential competitor. These results indicate that SMB could outcompete WAE under limiting prey conditions due to the aggressive nature of SMB, but resources may be partitioned at least along a temporal scale. ?? 2011 Taylor & Francis.

  15. Dworshak Reservoir Investigations: Trout, Bass and Forage Species, 1987 Annual Report.

    Energy Technology Data Exchange (ETDEWEB)

    Statler, David P.

    1988-05-01

    Dworshak Dam and Reservoir is a Corps of Engineers facility located on the North Fork Clearwater River 3.2 km upstream from the Mainstem Clearwater confluence. Since initial filling in 1971, conversion of 87 km of river habitat to a 6644 hectare impoundment has had a profound influence on resident fisheries. The Nez Perce Tribe and the Idaho Department of Fish and Game (IDFG) entered into separate intergovernmental agreements with the Bonneville Power Administration in a cooperative effort to study these impacts. The kokanee Oncorhynchus nerka assessment is included in the IDFG agreement, and is not addressed in this report. This project pertains primarily to rainbow trout Salmo gairdneri, smallmouth bass (Micropterus dolomieui), and forage species. For the period November 1987 through February 1988, an estimated 4339 angler-hours were expended to catch 430 rainbow trout. An estimated 20 bull trout Salvelinus confluentus, 4 smallmouth bass, and 4 suckers Catostomus spp. were also caught. Catch rates were generally poor through the period, at .091 fish per hour for all species combined (excluding kokanee). Shasta strain hatchery rainbow trout were dominant in the creel, comprising 53.9 percent of the catch, although this strain was last planted in the reservoir in June 1986. Bank anglers caught a higher percentage (93.5 percent) of the total catch of Shasta strain rainbows than Kamloops strain rainbows (33.3 percent). 11 refs., 4 figs., 6 tabs.

  16. Toxicity of three oil spill remediation techniques to the Australian bass Macquaria novemaculeata.

    Science.gov (United States)

    Cohen, A M; Nugegoda, D

    2000-10-01

    Australian bass, Macquaria novemaculeata, were exposed to the water accommodated fraction (WAF) of Bass Strait crude oil, dispersed crude oil, burnt crude oil, and 4-chlorophenol. The WAF of dispersed crude oil was the most toxic treatment with 96-h LC(50) values of 7. 15% (7.94% upper and 6.42% lower 95% CI) and 7.45% (8.26% upper and 6.71% lower 95% CI). The WAF of crude oil was less toxic, with 96-h LC(50) values of 43.72% (49.21% upper and 38.87% lower 95% CI) and 45.87% (51.51% upper and 40.97% lower 95% CI). The WAF of burnt crude oil was the least toxic treatment with 96-h LC(50) values of 49.81% (63.33% upper and 39.44% lower 95% CI) and 47.28% (59.72% upper and 37.62% lower 95% CI). Sublethal toxicity of the crude oil WAF and burnt crude oil WAF was observed at dilutions seven to eight times less than in the dispersed crude oil WAF.

  17. The Bass diffusion model on networks with correlations and inhomogeneous advertising

    CERN Document Server

    Bertotti, M L; Modanese, G

    2016-01-01

    The Bass model, which is an effective forecasting tool for innovation diffusion based on large collections of empirical data, assumes an homogeneous diffusion process. We introduce a network structure into this model and we investigate numerically the dynamics in the case of networks with link density $P(k)=c/k^\\gamma$, where $k=1, \\ldots , N$. The resulting curve of the total adoptions in time is qualitatively similar to the homogeneous Bass curve corresponding to a case with the same average number of connections. The peak of the adoptions, however, tends to occur earlier, particularly when $\\gamma$ and $N$ are large (i.e., when there are few hubs with a large maximum number of connections). Most interestingly, the adoption curve of the hubs anticipates the total adoption curve in a predictable way, with peak times which can be, for instance when $N=100$, between 10% and 60% of the total adoptions peak. This may allow to monitor the hubs for forecasting purposes. We also consider the case of networks with a...

  18. Cloning and Characterization of Largemouth Bass (Micropterus salmoides) Myostatin Encoding Gene and Its Promoter

    Institute of Scientific and Technical Information of China (English)

    LI Shengjie; BAI Junjie; WANG Lin

    2008-01-01

    Myostatin or GDF-8, a member of the transforming growth factor-β (TGF-β) superfamily, has been demonstrated to be a negative regulator of skeletal muscle mass in mammals. In the present study, we obtained a 5.64kb sequence of myostatin encoding gene and its promoter from largemouth bass (Micropterus salmoides). The myostatin encoding gene consisted of three exons (488bp, 371 bp and 1779bp, respectively) and two introns (390bp and 855 bp, respectively). The intron-exon boundaries were conservative in comparison with those of mammalian myostatin encoding genes, whereas the size of introns was smaller than that of mammals. Se- quence analysis of 1.569kb of the largemouth bass myostatin gene promoter region revealed that it contained two TATA boxes, one CAAT box and nine putative E-boxes. Putative muscle growth response elements for myocyte enhancer factor 2 (MEF2), serum response factor (SRF), activator protein 1 (API), etc.. and muscle-specific Mt binding site (MTBF) were also detected. Some of the transcription factor binding sites were conserved among five teleost species. This information will be useful for studying the tran- scriptional regulation of myostatin in fish.

  19. Evidence of estrogenic endocrine disruption in smallmouth and largemouth bass inhabiting Northeast U.S. National Wildlife Refuge waters: A reconnaissance study

    Science.gov (United States)

    Iwanowicz, Luke; Blazer, Vicki; Pinkney, A.E.; Guy, C.P.; Major, A.M.; Munney, K.; Mierzykowski, S.; Lingenfelser, S.; Secord, A.; Patnode, K.; Kubiak, T.J.; Stern, C.; Hahn, Cassidy M.; Iwanowicz, Deborah; Walsh, Heather L.; Sperry, Adam J.

    2016-01-01

    Intersex as the manifestation of testicular oocytes (TO) in male gonochoristic fishes has been used as an indicator of estrogenic exposure. Here we evaluated largemouth bass (Micropterus salmoides) or smallmouth bass (Micropterus dolomieu) form 19 National Wildlife Refuges (NWRs) in the Northeast U.S. inhabiting waters on or near NWR lands for evidence of estrogenic endocrine disruption. Waterbodies sampled included rivers, lakes, impoundments, ponds, and reservoirs. Here we focus on evidence of endocrine disruption in male bass evidenced by gonad histopathology including intersex or abnormal plasma vitellogenin (Vtg) concentrations. During the fall seasons of 2008–2010, we collected male smallmouth bass (n=118) from 12 sites and largemouth bass (n=173) from 27 sites. Intersex in male smallmouth bass was observed at all sites and ranged from 60% to 100%; in male largemouth bass the range was 0–100%. Estrogenicity, as measured using a bioluminescent yeast reporter, was detected above the probable no effects concentration (0.73 ng/L) in ambient water samples from 79% of the NWR sites. Additionally, the presence of androgen receptor and glucocorticoid receptor ligands were noted as measured via novel nuclear receptor translocation assays. Mean plasma Vtg was elevated (>0.2 mg/ml) in male smallmouth bass at four sites and in male largemouth bass at one site. This is the first reconnaissance survey of this scope conducted on US National Wildlife Refuges. The baseline data collected here provide a necessary benchmark for future monitoring and justify more comprehensive NWR-specific studies.

  20. Assessment of Caudal Fin Clips as a Non-lethal Technique for Predicting Muscle Tissue Mercury Concentrations in Largeouth Bass

    Science.gov (United States)

    The statistical relationship between total mercury (Hg) concentration in clips from the caudal fin and muscle tissue of largemouth bass (Micropterus salmoides) from 26 freshwater sites in Rhode Island, USA was developed and evaluated to determine the utility of fin clip analysis ...

  1. MEASUREMENT OF MECURY IN FISH SCALES AS AN ASSESSMENT METHOD FOR PREDICTING MUSCLE TISSUE MERCURY CNOCENTRATIONS IN LARGEMOUTH BASS

    Science.gov (United States)

    The relationship between total mercury (Hg) concentration in fish scales and in tissues of largemouth bass (Micropterus salmoides) from 20 freshwater sites was developed and evaluated to determine whether scale analysis would allow a non lethal and convenient method for predicti...

  2. Minimizing use of fish meal in sunshine bass diets using standard and new varieties of non-genetically modified soybeans

    Science.gov (United States)

    Improved plant ingredients are needed to support sustainable culture of carnivorous fish, such as hybrid striped bass (HSB). We are evaluating meals made from new strains of non-genetically-modified soybeans (non-GMO) with high protein and reduced anti-nutritional factors (ANFs) on HSB nutrient dige...

  3. Transforming growth factor-beta response to mycobacterial infection in striped bass Morone saxatilis and hybrid tilapia Oreochromis spp.

    Science.gov (United States)

    Harms, Craig A; Howard, Kristina E; Wolf, Jeffrey C; Smith, Stephen A; Kennedy-Stoskopf, Suzanne

    2003-10-15

    Striped bass (Morone saxatilis) and hybrid tilapia (Oreochromis spp.) were experimentally infected with Mycobacterium marinum. Splenic mononuclear cell transforming growth factor-beta (TGF-beta) mRNA was measured by reverse transcription quantitative-competitive PCR (RT-qcPCR). In histologic sections of liver and anterior kidney, the area of each section that was occupied by granulomas and the total area of each section were measured by computer-assisted image analysis and compared as a proportion (the granuloma proportion). Infected striped bass splenic mononuclear cell TGF-beta mRNA expression was significantly lower than uninfected controls, while for tilapia there was no significant difference between infected and control fish. Mycobacterial granuloma proportion of liver and anterior kidney sections was significantly greater for infected striped bass than tilapia. Three (of 10) infected tilapia with the most pronounced inflammatory response displayed a decrease in TGF-beta mRNA expression, similar to the overall striped bass response to mycobacterium challenge. Downregulation of TGF-beta and failure to modulate the immune response may be related to excessive inflammatory damage to organs observed in mycobacteria-sensitive fish species. PMID:12963276

  4. Synthesizing Evidence to Assess the Causes of Smallmouth Bass Declines at the Susquehanna and Juniata Rivers, Pennsylvania, USA

    Science.gov (United States)

    Unusual mortality events of smallmouth bass (SMB) have been observed in the Susquehanna River Basin annually since 2005 and have coincided with a decline in recruitment of young-of-year fish into the adult SMB population. In 2014, the Pennsylvania Department of Environmental Pro...

  5. Hypoxia affects performance traits and body composition of juvenile hybrid striped bass (Morone chrysops x M. saxatilis)

    Science.gov (United States)

    Performance traits and body composition of juvenile hybrid striped bass (Morone chrysops x M. saxatilis) in response to hypoxia were evaluated in replicate tanks maintained at constant dissolved oxygen concentrations that averaged 23.0 +/- 2.3%, 39.7 +/- 3.0%, and 105.5 +/- 9.5% dissolved oxygen sat...

  6. BACTERIOLOGICAL PROPERTIES OF MARINE WATER IN ADRIATIC FISH FARMS: ENUMERATION OF HETEROTROPHIC BACTERIA

    Directory of Open Access Journals (Sweden)

    Emin Teskeredžić

    2012-12-01

    Full Text Available Aquaculture is currently one of the fastest growing food production sectors in the world. Increase in nutrients and organic wastes lead to general deterioration of water quality. The problem of water quality is associated with both physical and chemical factors, as well as microbiological water quality. Heterotrophic bacteria play an important role in the process of decomposition of organic matter in water environment and indicate eutrophication process. Here we present our experience and knowledge on bacterial properties of marine water in the Adriatic fish farms with European sea bass (Dicentrarchus labrax L., 1758, with an emphasis on enumeration of heterotrophic bacteria in marine water. We applied two temperatures of incubation, as well as two methods for enumeration of heterotrophic bacteria: substrate SimPlate® test and spread plate method on conventional artificial media (Marine agar and Tryptic Soy agar with added NaCl. The results of analysis of bacteriological properties of marine water in the Adriatic fish farms showed that enumeration of heterotrophic bacteria in marine water depends on the applied incubation temperature and media for enumeration. At the same time, the incubation temperature of 22C favours more intense growth of marine heterotrophic bacteria, whereas a SimPlate test gives higher values of heterotrophic bacteria. Volatile values of heterotrophic bacteria during this research indicate a possible deterioration of microbiological water quality in the Adriatic fish farms and a need for regular monitoring of marine water quality.

  7. Comparison of heavy metal concentration of some marine fishes from Black and Aegean Seas

    Directory of Open Access Journals (Sweden)

    Makedonski Lubomir

    2015-06-01

    Full Text Available Major part of healthy human diet consist of marine fish and seafood products. And it is not surprising that there are numerous studies based on metal accumulation in various fish species. Fish may also be used for heavy metal monitoring programs of marine environments due to their easy sampling, sample preparation and chemical analysis. Concentrations of lead, cadmium, nickel, copper, manganese, zinc, iron, chromium, total mercury and total arsenic were determined in edible part of two commercially valuable fish Greek aquaculture species European sea bass (Dicentrarchus labrax and gilthead sea bream (Sparus aurata purchased from Bulgarian market during 2011. The concentration of metals was measured by atomic absorption spectrophotometry (AAS. The concentration of the heavy metals in examined fish species ranged as follow: Pb 0.008 - 0.013; Cd 0.0017 - 0.022; Ni 0.007 - 0.012; Cu 0.054 - 0.115; Mn 0.043 - 0.09; Zn 0.14 - 0.15; Fe 0.17 - 0.19; Cr 0.05 - 0.07; Hg 0.11 - 0.13; As 1.6 - 1.8 mg kg-1 wet weight, respectively.

  8. Application of Entropy and Fractal Dimension Analyses to the Pattern Recognition of Contaminated Fish Responses in Aquaculture

    Directory of Open Access Journals (Sweden)

    Harkaitz Eguiraun

    2014-11-01

    Full Text Available The objective of the work was to develop a non-invasive methodology for image acquisition, processing and nonlinear trajectory analysis of the collective fish response to a stochastic event. Object detection and motion estimation were performed by an optical flow algorithm in order to detect moving fish and simultaneously eliminate background, noise and artifacts. The Entropy and the Fractal Dimension (FD of the trajectory followed by the centroids of the groups of fish were calculated using Shannon and permutation Entropy and the Katz, Higuchi and Katz-Castiglioni’s FD algorithms respectively. The methodology was tested on three case groups of European sea bass (Dicentrarchus labrax, two of which were similar (C1 control and C2 tagged fish and very different from the third (C3, tagged fish submerged in methylmercury contaminated water. The results indicate that Shannon entropy and Katz-Castiglioni were the most sensitive algorithms and proved to be promising tools for the non-invasive identification and quantification of differences in fish responses. In conclusion, we believe that this methodology has the potential to be embedded in online/real time architecture for contaminant monitoring programs in the aquaculture industry.

  9. PHYTOPLANKTON ASSEMBLAGES AT FISH FARM IN MASLINOVA BAY (THE ISLAND OF BRAČ

    Directory of Open Access Journals (Sweden)

    Sanda Skejić

    2012-07-01

    Full Text Available The aim of this study was to establish phytoplankton composition at the sea bream (Sparus aurata and sea bass (Dicentrarchus labrax fish farm in the middle Adriatic Sea. The investigation was performed from September 2005 to September 2006 at a station located in Maslinova Bay at the island of Brač. Considering the whole research period, diatoms generally prevailed in terms of abundance while dinoflagellates were particularly abundant in June. Number of species of diatoms in comparison to dinoflagellates through the investigated period was similar. From 111 species of phytoplankton found, there were 55 species of Bacillariophyceae (diatoms, 47 species of Dinophyta (dinoflagellates, 5 species of Prymnesiophyceae, 3 Chrysophyceae and 1 Euglenophyta. Among the diatoms, the majority of species belonged to genus Chaetoceros. The most represented dinoflagellate genera were Oxytoxum and Gymnodinium. There were no considerable differences in phytoplankton composition with respect to different depths, but seasonal influence was significant. Biodiversity and abundance ranges of phytoplankton species indicated good water conditions and there were no evident alterations induced by the increased release of nutrients.

  10. Stable isotopes in juvenile marine fishes and their invertebrate prey from the Thames Estuary, UK, and adjacent coastal regions

    Science.gov (United States)

    Leakey, Chris D. B.; Attrill, Martin J.; Jennings, Simon; Fitzsimons, Mark F.

    2008-04-01

    Estuaries are regarded as valuable nursery habitats for many commercially important marine fishes, potentially providing a thermal resource, refuge from predators and a source of abundant prey. Stable isotope analysis may be used to assess relative resource use from isotopically distinct sources. This study comprised two major components: (1) development of a spatial map and discriminant function model of stable isotope variation in selected invertebrate groups inhabiting the Thames Estuary and adjacent coastal regions; and (2) analysis of stable isotope signatures of juvenile bass ( Dicentrarchus labrax), sole ( Solea solea) and whiting ( Merlangius merlangus) for assessment of resource use and feeding strategies. The data were also used to consider anthropogenic enrichment of the estuary and potential energetic benefits of feeding in estuarine nursery habitat. Analysis of carbon (δ 13C), nitrogen (δ 15N) and sulphur (δ 34S) isotope data identified significant differences in the 'baseline' isotopic signatures between estuarine and coastal invertebrates, and discriminant function analysis allowed samples to be re-classified to estuarine and coastal regions with 98.8% accuracy. Using invertebrate signatures as source indicators, stable isotope data classified juvenile fishes to the region in which they fed. Feeding signals appear to reflect physiological (freshwater tolerance) and functional (mobility) differences between species. Juvenile sole were found to exist as two isotopically-discrete sub-populations, with no evidence of mixing between the two. An apparent energetic benefit of estuarine feeding was only found for sole.

  11. Temporal variability in estuarine fish otolith elemental fingerprints: Implications for connectivity assessments

    Science.gov (United States)

    Reis-Santos, Patrick; Gillanders, Bronwyn M.; Tanner, Susanne E.; Vasconcelos, Rita P.; Elsdon, Travis S.; Cabral, Henrique N.

    2012-10-01

    The chemical composition of fish otoliths can provide valuable information for determining the nursery value of estuaries to adult populations of coastal fishes. However, understanding temporal variation in elemental fingerprints at different scales is important as it can potentially confound spatial discrimination among estuaries. Otolith elemental ratios (Li:Ca, Mg:Ca, Mn:Ca, Cu:Ca, Sr:Ca, Ba:Ca and Pb:Ca) of Platichthys flesus and Dicentrarchus labrax, from several estuaries along the Portuguese coast in two years and three seasons (spring, summer and autumn) within a year, were determined via Laser Ablation Inductively Coupled Plasma Mass Spectrometry. Elemental fingerprints varied significantly among years and seasons within a year but we achieved accurate classifications of juvenile fish to estuarine nursery of origin (77-96% overall cross-validated accuracy). Although elemental fingerprints were year-specific, variation among seasons did not hinder spatial discrimination. Estuarine fingerprints of pooled seasonal data were representative of the entire juvenile year class and attained high discrimination (77% and 80% overall cross-validated accuracy for flounder and sea bass, respectively). Incorporating seasonal variation resulted in up to an 11% increase in correct classification of individual estuaries, in comparison to seasons where accuracies were lowest. Overall, understanding the implications of temporal variations in otolith chemistry for spatial discrimination is key to establish baseline data for connectivity studies.

  12. State of the art of Italian aquaculture

    Directory of Open Access Journals (Sweden)

    P. Melotti

    2010-04-01

    Full Text Available According to aquaculture production statistics published by FEAP (2007, Italy is the fifth largest fish producer in the European Union having a total quantity for 2006 estimated around 60,000 t. This data is exclusively referred to rainbow trout (Oncorhynchus mykiss, seabream (Sparus aurata, seabass (Dicentrarchus labrax and European eel (Anguilla anguilla but even if we consider the total aquaculture production elaborated by ISMEA (2006 through year 2005 including sturgeons, carps, striped bass, catfish and ornamental fish, Italy ranks fourth with 69,100 t after Norway (655,364 t, Great Britain (141,793 t and Greece (83,600 t. Over the last 15 years, Italian finfish production has known a decrease related to all the species mainly reared (trout, eel, carps, catfish except for the eurhyaline species that have had an important expansion (Table 1. Based on these considerations, in this work we describe the main features of fish aquaculture in Italy focalizing the attention to the single sectors of the farmed species and their trend for the future.

  13. Status and future perspectives of vaccines for industrialised fin-fish farming.

    Science.gov (United States)

    Brudeseth, Bjørn Erik; Wiulsrød, Rune; Fredriksen, Børge Nilsen; Lindmo, Karine; Løkling, Knut-Egil; Bordevik, Marianne; Steine, Nils; Klevan, Are; Gravningen, Kjersti

    2013-12-01

    Fin fish farming is developing from extensive to intensive high industrial scale production. Production of fish in high-density growth conditions requires effective vaccines in order to control persistent and emerging diseases. Vaccines can also have significant positive impact on the reduced usage of antibiotics. This was demonstrated when vaccines were introduced in Norway for Atlantic salmon (Salmo salar) in the late eighties and early nineties, resulting in a rapid decline of antibiotics consumption. The present review will focus on current vaccine applications for farmed industrialized fish species such as Atlantic salmon, coho salmon (Oncorhynchus kisutch), rainbow trout (Oncorhynchus mykiss), ayu (Plecoglossus altivelis), cod (Gadus morhua), sea bass (Dicentrarchus labrax), gilt-head sea bream (Sparus aurata), yellowtail (Seriola quinqueradiata), great amberjack (Seriola dumerili), barramundi (Lates calcarifer), japanese flounder (Paralichythys olivaceus), turbot (Scophthalmus maximus), red sea bream (Pagrus major), rock bream (Oplegnathus fasciatus), seven band grouper (Epinephelus septemfasciatus), striped catfish (Pangasianodon hypophthalmus), channel catfish (Ictalurus punctatus) and tilapia (Oreochromis niloticus). This paper will review the current use of licensed vaccines in fin fish farming and describe vaccine administration regimes including immersion, oral and injection vaccination. Future trends for inactivated-, live attenuated - and DNA - vaccines will also be discussed.

  14. Experiments on the use of sound as a fish deterrent

    International Nuclear Information System (INIS)

    This report describes a series of experimental studies into the potential use of acoustic stimuli to deter fish from water intakes at thermal and hydroelectric power stations. The aim was to enlarge the range of candidate signals for testing, and to apply these in more rigorous laboratory trials and to a wider range of estuarine and marine fish species than was possible in previous initial preliminary studies. The trials were also required to investigate the degree to which fish might become habituated to the sound signals, consequently reducing their effectiveness. The species of fish which were of interest in this study were the Atlantic salmon (Salmo salar), sea trout (Salmo trutta), the shads (Alosa fallax, A. alosa), the European eel (Anguilla anguilla), bass (Dicentrarchus labrax), herring (Clupea harengus), whiting (Merlangius merlangus) and cod (Gadus morhua). All of these species are considered to be of conservation and/or commercial importance in Britain today and are potentially vulnerable to capture by nuclear, fossil-fuelled and tidal generating stations. Based on the effectiveness of the signals observed in these trials, a properly developed and sited acoustic fish deterrent system is expected to reduce fish impingement significantly at water intakes. Field trials at an estuarine power station are recommended. (author)

  15. Status and future perspectives of vaccines for industrialised fin-fish farming.

    Science.gov (United States)

    Brudeseth, Bjørn Erik; Wiulsrød, Rune; Fredriksen, Børge Nilsen; Lindmo, Karine; Løkling, Knut-Egil; Bordevik, Marianne; Steine, Nils; Klevan, Are; Gravningen, Kjersti

    2013-12-01

    Fin fish farming is developing from extensive to intensive high industrial scale production. Production of fish in high-density growth conditions requires effective vaccines in order to control persistent and emerging diseases. Vaccines can also have significant positive impact on the reduced usage of antibiotics. This was demonstrated when vaccines were introduced in Norway for Atlantic salmon (Salmo salar) in the late eighties and early nineties, resulting in a rapid decline of antibiotics consumption. The present review will focus on current vaccine applications for farmed industrialized fish species such as Atlantic salmon, coho salmon (Oncorhynchus kisutch), rainbow trout (Oncorhynchus mykiss), ayu (Plecoglossus altivelis), cod (Gadus morhua), sea bass (Dicentrarchus labrax), gilt-head sea bream (Sparus aurata), yellowtail (Seriola quinqueradiata), great amberjack (Seriola dumerili), barramundi (Lates calcarifer), japanese flounder (Paralichythys olivaceus), turbot (Scophthalmus maximus), red sea bream (Pagrus major), rock bream (Oplegnathus fasciatus), seven band grouper (Epinephelus septemfasciatus), striped catfish (Pangasianodon hypophthalmus), channel catfish (Ictalurus punctatus) and tilapia (Oreochromis niloticus). This paper will review the current use of licensed vaccines in fin fish farming and describe vaccine administration regimes including immersion, oral and injection vaccination. Future trends for inactivated-, live attenuated - and DNA - vaccines will also be discussed. PMID:23769873

  16. Striped bass annual site fidelity and habitat utilization in J. Strom Thurmond Reservoir, South Carolina-Georgia

    Science.gov (United States)

    Young, S.P.; Isely, J.J.

    2002-01-01

    Forty-eight adult striped bass Morone saxatilis (3.2-19.1 kg) were captured by electrofishing in the tailrace of Richard B. Russell Dam and in the upper reaches of two major tributaries; they were implanted with temperature-sensitive radio transmitters and tracked approximately bimonthly for 20 months. As J. Strom Thurmond Reservoir downstream from the dam became thermally stratified in May, fish vacated the tributaries. From June to October, all striped bass were found within the reservoir's historical Savannah River channel. By August, most of the instrumented fish were found in the upper section of the reservoir, where optimal habitat was available throughout the summer owing to cool, artificially oxygenated hypolimnetic discharges from Richard B. Russell Dam. In mid-October the reservoir destratified, and fish dispersed from their up-reservoir summering areas and redistributed themselves throughout the reservoir. During early winter, the striped bass returned to tributary habitat or down-reservoir areas and generally used these locations throughout the winter. The fish exhibited a high degree of site fidelity to their summering areas, source tributaries (after fall dispersal and throughout the winter), and spring spawning areas. Mean movement rates were highest in the spring and fall, corresponding to the migration from tributaries in May and the return migration after fall dispersal. Mean movement rates were lowest in summer and winter, corresponding to the periods of high fidelity to summering and wintering areas. The average monthly temperatures and dissolved oxygen concentrations in areas used by striped bass were 19.0-20.4??C and 4.86-6.44 mg/L during May-October, which corresponded to average monthly habitat suitability index values of 0.76-0.98. Striped bass avoided temperatures above 25.1??C and dissolved oxygen concentrations less than 2.3 mg/L.

  17. Study on Mitochondrial DNA Cytochrome b Gene of Chinese sea bass,%Study on Mitochondrial DNA Cytochrome b Gene of Chinese sea bass, Lateolabrax sp.

    Institute of Scientific and Technical Information of China (English)

    高天翔; 张秀梅; 陈大刚; 张美昭; 任一平; 张亚平

    2001-01-01

    参考鳗鲡等鱼类线粒体DNA序列进行了中国花鲈线粒体DNA细胞色素b基因片断的引物设计、PCR扩增及其序列测定。得到中国花鲈的碱基序列为410bp,其A、T、G、C含量分别为101bp(24.63%)、112bp(27.32%)、72bp(17.56%)、125bp(30.49%),与鳗鲡等其他鱼类相同基因片断序列碱基含量相似。%The primers of mitochondrial DNA cytochrome b gene of C hinesesea bass were designed by referencing the sequences of Anguilla speci es. We successfully amplified and sequenced the 410 base pairs that encode the c ytochrome b gene of mitochondrial DNA. The A, T, G and C contents of the sequenc e were 101bp (24.63%), 112bp (27.32%), 72bp (17.56%) and 125bp (30 .49%) respectively, similar to the sequences of other species.

  18. LAS CLAVES DE LA INNOVACIÓN DE SAUL BASS EN LA SECUENCIA DE TÍTULOS DE CRÉDITO DE VÉRTIGO

    OpenAIRE

    Alcaraz Pagán, Mª De Las Nieves

    2016-01-01

    [EN] Saul Bass is considered as the father of contemporary title sequences. The main contribution of this work consists in verifying the close correspondences between Bass' deviced iconography, figures and visual motifs to summarize and synthesise the profound sense of the story told by the film, preceded by their sequences, since it is this idea that is found as the base of what has been established as the turning point and conceptual axis around his innovation. This is something until nowad...

  19. An ovary transcriptome for all maturational stages of the striped bass (Morone saxatilis), a highly advanced perciform fish

    OpenAIRE

    Reading Benjamin J; Chapman Robert W; Schaff Jennifer E; Scholl Elizabeth H; Opperman Charles H; Sullivan Craig V

    2012-01-01

    Abstract Background The striped bass and its relatives (genus Morone) are important fisheries and aquaculture species native to estuaries and rivers of the Atlantic coast and Gulf of Mexico in North America. To open avenues of gene expression research on reproduction and breeding of striped bass, we generated a collection of expressed sequence tags (ESTs) from a complementary DNA (cDNA) library representative of their ovarian transcriptome. Results Sequences of a total of 230,151 ESTs (51,259...

  20. Edward W. Taylor / Patricia Cranton, and Associates (Hrsg.): The Handbook of Transformative Learning, Theory, Research, and Practice. San Francisco, CA: Jossey-Bass 2012 [...] [Sammelrezension

    OpenAIRE

    Fuhr, Thomas

    2012-01-01

    Sammelrezension von: 1. Edward W. Taylor / Patricia Cranton, and Associates (Hrsg.): The Handbook of Transformative Learning, Theory, Research, and Practice, San Francisco, CA: Jossey-Bass 2012 (598 S.; ISBN 978-1-111-21891-4) 2. Jack Mezirow / Edward W. Taylor, and Associates (Hrsg.): Transformative Learning in Practice, Insights from Community, Workplace, and Higher Education, San Francisco, CA: Jossey-Bass 2009 (303 S.; ISBN 978-0-470-25790-6)

  1. Identification of largemouth bass virus in the introduced Northern Snakehead inhabiting the Chesapeake Bay watershed.

    Science.gov (United States)

    Iwanowicz, L; Densmore, C; Hahn, C; McAllister, P; Odenkirk, J

    2013-09-01

    The Northern Snakehead Channa argus is an introduced species that now inhabits the Chesapeake Bay. During a preliminary survey for introduced pathogens possibly harbored by these fish in Virginia waters, a filterable agent was isolated from five specimens that produced cytopathic effects in BF-2 cells. Based on PCR amplification and partial sequencing of the major capsid protein (MCP), DNA polymerase (DNApol), and DNA methyltransferase (Mtase) genes, the isolates were identified as Largemouth Bass virus (LMBV). Nucleotide sequences of the MCP (492 bp) and DNApol (419 pb) genes were 100% identical to those of LMBV. The nucleotide sequence of the Mtase (206 bp) gene was 99.5% identical to that of LMBV, and the single nucleotide substitution did not lead to a predicted amino acid coding change. This is the first report of LMBV from the Northern Snakehead, and provides evidence that noncentrarchid fishes may be susceptible to this virus.

  2. Identification of largemouth bass virus in the introduced Northern snakehead inhabiting the Cheasapeake Bay watershed

    Science.gov (United States)

    Iwanowicz, Luke R.; Densmore, Christine L.; Hahn, Cassidy M.; McAllister, Phillip; Odenkirk, John

    2013-01-01

    The Northern Snakehead Channa argus is an introduced species that now inhabits the Chesapeake Bay. During a preliminary survey for introduced pathogens possibly harbored by these fish in Virginia waters, a filterable agent was isolated from five specimens that produced cytopathic effects in BF-2 cells. Based on PCR amplification and partial sequencing of the major capsid protein (MCP), DNA polymerase (DNApol), and DNA methyltransferase (Mtase) genes, the isolates were identified as Largemouth Bass virus (LMBV). Nucleotide sequences of the MCP (492 bp) and DNApol (419 pb) genes were 100% identical to those of LMBV. The nucleotide sequence of the Mtase (206 bp) gene was 99.5% identical to that of LMBV, and the single nucleotide substitution did not lead to a predicted amino acid coding change. This is the first report of LMBV from the Northern Snakehead, and provides evidence that noncentrarchid fishes may be susceptible to this virus.

  3. Meralgia paresthetica: a result of tight new trendy low cut trousers ('taille basse').

    Science.gov (United States)

    Moucharafieh, Ramzi; Wehbe, Joseph; Maalouf, Ghassan

    2008-04-01

    Meralgia paresthetica is an entrapment neuropathy involving the lateral femoral cutaneous nerve of the thigh. Patients complain of a persistent burning sensation, tingling and aching pain, and hypersensitivity or hyposensitivity in the anterolateral aspect of the thigh. Numerous direct and indirect causes for the disease have been suggested in the literature. We present 12 cases that were diagnosed to have meralgia paresthetica due to tight new fashion low cut trousers ('taille basse'). The diagnosis was confirmed by injecting a small amount of a short acting local anesthetic around the lateral femoral cutaneous nerve which alleviated the symptoms for several hours. Electrophysiologic studies were sensitive in 83.3% of the cases. All cases were treated successfully using conservative methods, namely avoiding tight trousers, local steroid infiltration and weight reduction.

  4. Bioenergetics estimate of the effects of stocking density on hatchery production of smallmouth bass fingerlings

    Science.gov (United States)

    Robel, G.L.; Fisher, W.L.

    1999-01-01

    Production of and consumption by hatchery-reared tingerling (age-0) smallmouth bass Micropterus dolomieu at various simulated stocking densities were estimated with a bioenergetics model. Fish growth rates and pond water temperatures during the 1996 growing season at two hatcheries in Oklahoma were used in the model. Fish growth and simulated consumption and production differed greatly between the two hatcheries, probably because of differences in pond fertilization and mortality rates. Our results suggest that appropriate stocking density depends largely on prey availability as affected by pond fertilization and on fingerling mortality rates. The bioenergetics model provided a useful tool for estimating production at various stocking density rates. However, verification of physiological parameters for age-0 fish of hatchery-reared species is needed.

  5. Maison forte du Boisset : panoramique complet de la basse-cour

    OpenAIRE

    Pouyllau, Stéphane

    1996-01-01

    Panoramique de 360° réalisé par superposition d'images de la basse cour de la maison forte du boisset en 1998. Le Boisset est une maison forte reconstruite au XVe siècle (entre 1438 et 1480). Le site est entouré d'un anneau fossoyé. Certaines structures (au dessous du bâti actuel) et les fossés datent probablement de la fin du XIIIe siècle. Le premier site est à mettre en liaison avec la présence d'un lignage repérable dans ce secteur en 1260 : la famille des Brun de Boisset. Cette famille es...

  6. Erosion by the Storm Helena on Basse Terre Island - Guadeloupe: Past Climatic Consequences

    Science.gov (United States)

    Delacourt, C.; Allemand, P.; Lajeunesse, E.; Devauchelle, O.

    2012-12-01

    It is generally accepted that extreme meteorological events are the main morphogenic agents in the tropical belt. In the lesser Antilles arc, these events have an average return period of 4 to 5 years since they are registered. We measured the erosive effects of the tropical storm Helena that hit the volcanic Basse Terre Island (Guadeloupe - Lesser Antilles Arc) on October 24, 1963, on aerial images taken few weeks after the event by the French "Institut Géographique National"(IGN). On the images, 253 landslides, which occured during the storm, were identified and mapped. These landslides were located in the center of the island in the watershed with major relief. If the thickness of the landslide was 1m, i.e. less than the thickness of the weathered layer, the total volume of displaced sediments corresponded to denudation of 1.4mm on the watersheds concerned by landsliding. The total volume of eroded material since the emplacement of lavas has been estimated for each watershed by measuring the volume of valleys. The average velocity of denudation has been estimated to 0.5mm/y since the lava emplacement. If the storm Helena is characteristic of the storms that hit Basse Terre during the Quaternary, the period of return of such extreme meteorological event should be nearer 15 years rather than 4 to 5 years. This longer value of the period of return is coherent with the lower surface temperatures recorded before the Holocene in the Caribbean and Atlantic oceans, which produced less storms then present-day conditions.

  7. A strain of Siniperca chuatsi rhabdovirus causes high mortality among cultured Largemouth Bass in South China.

    Science.gov (United States)

    Ma, Dongmei; Deng, Guocheng; Bai, Junjie; Li, Shengjie; Yu, Lingyun; Quan, Yingchun; Yang, Xiaojing; Jiang, Xiaoyan; Zhu, Zemin; Ye, Xing

    2013-09-01

    In April 2011, 40% mortality of Largemouth Bass Micropterus salmoides juveniles occurred at a farm of Zhongshan City, Guangdong Province, China. Infected fish became lethargic, exhibited corkscrew and irregular swimming, and developed a distended abdomen and crooked body. Fish began to die within 2 d after the appearance of clinical signs. In order to analyze the pathogeny and diagnose the disease earlier, observation of clinical signs, cell infection, titer calculation, electron microscopy, immersion infection assay for fish, and nucleotide sequence analysis were carried out. Fathead minnow (FHM) cell cultures, inoculated with filtrate of liver and spleen homogenates from the diseased fish, developed the obvious cytopathic effect 46 h after inoculation in the primary culture and 24 h at the first passage. Typical rhabdovirus particles, 115-143 nm in length and 62-78 nm in diameter, were observed in infected FHM cells by direct transmission electron microscopy. The isolated virus produced a titer of 10(7.15) TCID50/mL. Immersion-Fish infected with the virus had similar clinical signs and 80% mortality with 10(2.5) LD50/mL. The data indicated that the rhabdovirus was the lethal pathogeny of the current disease. Based on nucleoprotein-gene nucleotide sequence multiple alignment analysis, the newly isolated virus is a strain of Siniperca chuatsi rhabdovirus (SCRV) under family Rhabdoviridae, which was initially isolated from Mandarin Fish Siniperca chuatsi. Up to the present, at least four virus strains have been isolated from diseased Largemouth Bass, which have had different clinical signs. Comparison of the clinical signs can help in an early diagnosis of the disease. PMID:23915177

  8. Methylmercury-induced changes in gene transcription associated with neuroendocrine disruption in largemouth bass (Micropterus salmoides)

    Science.gov (United States)

    Richter, Catherine A.; Martyniuk, Christopher J.; Annis, Mandy L.; Brumbaugh, William G.; Chasar, Lia C.; Denslow, Nancy D.; Tillitt, Donald E.

    2014-01-01

    Methyl-mercury (MeHg) is a potent neuroendocrine disruptor that impairs reproductive processes in fish. The objectives of this study were to (1) characterize transcriptomic changes induced by MeHg exposure in the female largemouth bass (LMB) hypothalamus under controlled laboratory conditions, (2) investigate the health and reproductive impacts of MeHg exposure on male and female largemouth bass (LMB) in the natural environment, and (3) identify MeHg-associated gene expression patterns in whole brain of female LMB from MeHg-contaminated habitats. The laboratory experiment was a single injection of 2.5 μg MeHg/g body weight for 96 h exposure. The field survey compared river systems in Florida, USA with comparably lower concentrations of MeHg (Wekiva, Santa Fe, and St. Johns Rivers) in fish and one river system with LMB that contained elevated concentrations of MeHg (St. Marys River). Microarray analysis was used to quantify transcriptomic responses to MeHg exposure. Although fish at the high-MeHg site did not show overt health or reproductive impairment, there were MeHg-responsive genes and pathways identified in the laboratory study that were also altered in fish from the high-MeHg site relative to fish at the low-MeHg sites. Gene network analysis suggested that MeHg regulated the expression targets of neuropeptide receptor and steroid signaling, as well as structural components of the cell. Disease-associated gene networks related to MeHg exposure, based upon expression data, included cerebellum ataxia, movement disorders, and hypercalcemia. Gene responses in the CNS are consistent with the documented neurotoxicological and neuroendocrine disrupting effects of MeHg in vertebrates.

  9. Identification and expression of GnRH2 and GnRH3 in the black sea bass (Centropristis striata), a hermaphroditic teleost.

    Science.gov (United States)

    Morin, Scott J; Decatur, Wayne A; Breton, Timothy S; Marquis, Timothy J; Hayes, Mary K; Berlinsky, David L; Sower, Stacia A

    2015-04-01

    We cloned two cDNAs for two gonadotropin-releasing hormones, GnRH2 (chicken GnRH-II) and GnRH3 (salmon GnRH), respectively, from the black sea bass (Centropristis striata). Black sea bass are protogynous hermaphroditic teleosts that change from females to males between 2 and 5 years of age. Similar to other GnRH precursors, the precursors of black sea bass GnRH2 and GnRH23 consisted of a signal peptide, decapeptide, a downstream processing site, and a GnRH-associated peptide. Our analyses failed to identify GnRH1. GnRH3 precursor transcript was more widely distributed in a variety of tissues compared with GnRH2. Further examination of GnRH expression and gonadal histology was done in black sea bass from three different size groups: small (11.4-44.1 g), medium (179.4-352.2 g) and large (393.8-607.3 g). Interestingly, GnRH3 expression occurred only in the pituitaries of males in the small and medium groups compared with expression of GnRH2. Future functional studies of the sea bass GnRHs will be valuable in elucidating the potential underlying neuroendocrine mechanisms of black sea bass reproduction and may ultimately contribute to management advances in this commercially important fish. PMID:25255937

  10. Transcriptional networks associated with the immune system are disrupted by organochlorine pesticides in largemouth bass (Micropterus salmoides) ovary.

    Science.gov (United States)

    Martyniuk, Christopher J; Doperalski, Nicholas J; Feswick, April; Prucha, Melinda S; Kroll, Kevin J; Barber, David S; Denslow, Nancy D

    2016-08-01

    Largemouth bass (Micropterus salmoides) inhabiting Lake Apopka, Florida are exposed to high levels of persistent organochlorine pesticides (OCPs) and dietary uptake is a significant route of exposure for these apex predators. The objectives of this study were to determine the dietary effects of two organochlorine pesticides (p, p'-dichlorodiphenyldichloroethylene; p, p' DDE and methoxychlor; MXC) on the reproductive axis of largemouth bass. Reproductive bass (late vitellogenesis) were fed one of the following diets: control pellets, 125ppm p, p'-DDE, or 10ppm MXC (mg/kg) for 84days. Due to the fact that both p,p' DDE and MXC have anti-androgenic properties, the anti-androgenic pharmaceutical flutamide was fed to a fourth group of largemouth bass (750ppm). Following a 3 month exposure, fish incorporated p,p' DDE and MXC into both muscle and ovary tissue, with the ovary incorporating 3 times more organochlorine pesticides compared to muscle. Endpoints assessed were those related to reproduction due to previous studies demonstrating that these pesticides impact the reproductive axis and we hypothesized that a dietary exposure would result in impaired reproduction. However, oocyte distribution, gonadosomatic index, plasma vitellogenin, and plasma sex steroids (17β-estradiol, E2 and testosterone, T) were not different between control animals and contaminant-fed largemouth bass. Moreover, neither p, p' DDE nor MXC affected E2 or T production in ex vivo oocyte cultures from chemical-fed largemouth bass. However, both pesticides did interfere with the normal upregulation of androgen receptor that is observed in response to human chorionic gonadotropin in ex vivo cultures, an observation that may be related to their anti-androgenic properties. Transcriptomics profiling in the ovary revealed that gene networks related to cell processes such as leukocyte cell adhesion, ossification, platelet function and inhibition, xenobiotic metabolism, fibrinolysis, and thermoregulation

  11. Transcriptional networks associated with the immune system are disrupted by organochlorine pesticides in largemouth bass (Micropterus salmoides) ovary.

    Science.gov (United States)

    Martyniuk, Christopher J; Doperalski, Nicholas J; Feswick, April; Prucha, Melinda S; Kroll, Kevin J; Barber, David S; Denslow, Nancy D

    2016-08-01

    Largemouth bass (Micropterus salmoides) inhabiting Lake Apopka, Florida are exposed to high levels of persistent organochlorine pesticides (OCPs) and dietary uptake is a significant route of exposure for these apex predators. The objectives of this study were to determine the dietary effects of two organochlorine pesticides (p, p'-dichlorodiphenyldichloroethylene; p, p' DDE and methoxychlor; MXC) on the reproductive axis of largemouth bass. Reproductive bass (late vitellogenesis) were fed one of the following diets: control pellets, 125ppm p, p'-DDE, or 10ppm MXC (mg/kg) for 84days. Due to the fact that both p,p' DDE and MXC have anti-androgenic properties, the anti-androgenic pharmaceutical flutamide was fed to a fourth group of largemouth bass (750ppm). Following a 3 month exposure, fish incorporated p,p' DDE and MXC into both muscle and ovary tissue, with the ovary incorporating 3 times more organochlorine pesticides compared to muscle. Endpoints assessed were those related to reproduction due to previous studies demonstrating that these pesticides impact the reproductive axis and we hypothesized that a dietary exposure would result in impaired reproduction. However, oocyte distribution, gonadosomatic index, plasma vitellogenin, and plasma sex steroids (17β-estradiol, E2 and testosterone, T) were not different between control animals and contaminant-fed largemouth bass. Moreover, neither p, p' DDE nor MXC affected E2 or T production in ex vivo oocyte cultures from chemical-fed largemouth bass. However, both pesticides did interfere with the normal upregulation of androgen receptor that is observed in response to human chorionic gonadotropin in ex vivo cultures, an observation that may be related to their anti-androgenic properties. Transcriptomics profiling in the ovary revealed that gene networks related to cell processes such as leukocyte cell adhesion, ossification, platelet function and inhibition, xenobiotic metabolism, fibrinolysis, and thermoregulation

  12. Pathogenicity of Aeromonas hydrophila isolated from the Malaysian Sea against coral (Turbinaria sp.) and sea bass (Lates calcarifer).

    Science.gov (United States)

    Hamid, Rahimi; Ahmad, Asmat; Usup, Gires

    2016-09-01

    A study was carried out to determine the pathogenicity (hemolytic activity) on corals (Turbinaria sp.) and sea bass (Lates calcarifer) of Aeromonas hydrophila from water, sediment, and coral. Samples were collected from coastal water and coral reef areas. One hundred and sixty-two isolates were successfully isolated. Out of 162, 95 were from seawater, 49 from sediment, and 18 from coral. Sixteen isolates were picked and identified. Isolates were identified using a conventional biochemical test, the API 20NE kit, and 16S rRNA nucleotide sequences. Hemolytic activity was determined. Out of 16 isolates, 14 isolates were β-hemolytic and two isolates were non-hemolytic. Corals infected with A. hydrophila suffered bleaching. Similar effect was observed for both hemolytic and non-hemolytic isolates. Intramuscular injection of A. hydrophila into sea bass resulted in muscular bleeding and death. Higher infection rates were obtained from hemolytic compared to non-hemolytic strains of A. hydrophila isolates. PMID:27221587

  13. The early stress responses in fish larvae.

    Science.gov (United States)

    Pederzoli, Aurora; Mola, Lucrezia

    2016-05-01

    During the life cycle of fish the larval stages are the most interesting and variable. Teleost larvae undergo a daily increase in adaptability and many organs differentiate and become active. These processes are concerted and require an early neuro-immune-endocrine integration. In larvae communication among the nervous, endocrine and immune systems utilizes several known signal molecule families which could be different from those of the adult fish. The immune-neuroendocrine system was studied in several fish species, among which in particular the sea bass (Dicentrarchus labrax), that is a species of great commercial interest, very important in aquaculture and thus highly studied. Indeed the immune system of this species is the best known among marine teleosts. In this review the data on main signal molecules of stress carried out on larvae of fish are considered and discussed. For sea bass active roles in the early immunological responses of some well-known molecules involved in the stress, such as ACTH, nitric oxide, CRF, HSP-70 and cortisol have been proposed. These molecules and/or their receptors are biologically active mainly in the gut before complete differentiation of gut-associated lymphoid tissue (GALT), probably acting in an autocrine/paracrine way. An intriguing idea emerges from all results of these researches; the molecules involved in stress responses, expressed in the adult cells of the hypothalamic-pituitary axis, during the larval life of fish are present in several other localizations, where they perform probably the same role. It may be hypothesized that the functions performed by hypothalamic-pituitary system are particularly important for the survival of the larva and therefore they comprises several other localizations of body. Indeed the larval stages of fish are very crucial phases that include many physiological changes and several possible stress both internal and environmental. PMID:26968620

  14. Quantitative trait loci involved in sex determination and body growth in the gilthead sea bream (Sparus aurata L. through targeted genome scan.

    Directory of Open Access Journals (Sweden)

    Dimitrios Loukovitis

    Full Text Available Among vertebrates, teleost fish exhibit a considerably wide range of sex determination patterns that may be influenced by extrinsic parameters. However even for model fish species like the zebrafish Danio rerio the precise mechanisms involved in primary sex determination have not been studied extensively. The zebrafish, a gonochoristic species, is lacking discernible sex chromosomes and the sex of juvenile fish is difficult to determine. Sequential protandrous hermaphrodite species provide distinct determination of the gender and allow studying the sex determination process by looking at the mechanism of sex reversal. This is the first attempt to understand the genetic basis of phenotypic variation for sex determination and body weight in a sequential protandrous hermaphrodite species, the gilthead sea bream (Sparus aurata. This work demonstrates a fast and efficient strategy for Quantitative Trait Loci (QTL detection in the gilthead sea bream, a non-model but target hermaphrodite fish species. Therefore a comparative mapping approach was performed to query syntenies against two other Perciformes, the European sea bass (Dicentrarchus labrax, a gonochoristic species and the Asian sea bass (Lates calcarifer a protandrous hermaphrodite. In this manner two significant QTLs, one QTL affecting both body weight and sex and one QTL affecting sex, were detected on the same linkage group. The co-segregation of the two QTLs provides a genomic base to the observed genetic correlation between these two traits in sea bream as well as in other teleosts. The identification of QTLs linked to sex reversal and growth, will contribute significantly to a better understanding of the complex nature of sex determination in S. aurata where most individuals reverse to the female sex at the age of two years through development and maturation of the ovarian portion of the gonad and regression of the testicular area. [Genomic sequences reported in this manuscript have been

  15. Dynamics of polychlorinated biphenyls in striped bass from the Hudson river. III. tissue disposition and routes for elimination

    International Nuclear Information System (INIS)

    Striped bass were exposed to 14C-polychlorinated biphenyls (PCB) in single-dose and multiple-dose experiments. Samples were analyzed to determine rate constants for PCB elimination from individual tissues, PCB concentration in tissues, the proportion of the PCB burden retained and the proportion of the cumulative dose retained by each tissue at various times after exposure. An experiment was also conducted to determine both the potential for secondary PCB uptake in dietary exposure studies and the relative tissue disposition of PCBs assimilated from dietary sources as compared to direct water uptake. PCBs were present in the tissues of striped bass within 6 h after administration of a single dose. Certain tissue compartments, such as the liver/gall bladder, accumulated PCBs over a period of 48 h even though the whole-body burden had decreased between 24 and 48 h. Except for the gills, elimination rate constants for all tissues were similar and were to the whole body elimination rate constant. Elimination during the first few hours following exposure to PCBs may be due to equilibrium partitioning from the gill to the environment. The multiple-dose study showed that PCB burdens in striped bass continued to increase with dosing. However, tissue-specific rate constants for PCB elimination led to an increased flux of PCB out of tissues, and an overall decline in the percent of the cumulative dose remaining in the body 48 h after administration of each dose. The most likely route for PCB elimination from striped bass was from tissues to the liver and thence to the intestine via the bile. These were no differences in the tissue disposition of PCB related to route of exposure

  16. Largemouth bass (Micropterus salmoides) and striped mullet (Mugil cephalus) as vectors of contaminants to human consumers in northwest Florida

    Science.gov (United States)

    Karouna-Renier, Natalie K.; Snyder, Richard A.; Lange, Ted; Gibson, Suzanne; Allison, Jeffrey G.; Wagner, Matthew E.; Rao, K. Ranga

    2011-01-01

    The health benefits of regular consumption of fish and seafood have been espoused for many years. However, fish are also a potential source of environmental contaminants that have well known adverse effects on human health. We investigated the consumption risks for largemouth bass (Micropterus salmoides; n = 104) and striped mullet (Mugil cephalus; n = 170), two commonly harvested and consumed fish species inhabiting fresh and estuarine waters in northwest Florida. Skinless fillets were analyzed for total mercury, inorganic arsenic, polychlorinated dibenzo-p-dioxins and polychlorinated dibenzofurans (PCDD/F), polychlorinated biphenyls (PCBs), and organochlorine pesticides. Contaminant levels were compared to screening values (SV) calculated using U.S. Environmental Protection Agency (EPA) recommendations for establishing consumption advisories. Largemouth bass were found to contain high levels of total mercury at all sampling locations (0.37-0.89 ug/g) and one location exhibited elevated total PCBs (39.4 ng/g). All of the samples exceeded Florida fish consumption advisory trigger levels for total mercury and one location exceeded the U.S. EPA SV for total PCBs. As a result of the high mercury levels, the non-cancer health risks (hazard index-HI) for bass were above 1 for all locations. Striped mullet from several locations with known point sources contained elevated levels of PCBs (overall range 3.4-59.3 ng/g). However, total mercury levels in mullet were low. Eight of the 16 mullet sampling locations exceeded the U.S. EPA SV for total PCBs and two locations exceeded an HI of 1 due to elevated PCBs. Despite the elevated levels of total PCBs in some samples, only two locations exceeded the acceptable cancer risk range and therefore cancer health risks from consumption of bass and mullet were determined to be low at most sampling locations.

  17. Interactive effects of p,p'-dichlorodiphenyldichloroethylene and methoxychlor on hormone synthesis in largemouth bass ovarian cultures.

    Science.gov (United States)

    Borgert, Christopher J; Gross, Timothy S; Guiney, Patrick D; Osimitz, Tomas G; Price, Bertram; Wells, Christopher

    2004-08-01

    p,p'-Dichlorodiphenyldichloroethylene (DDE) and methoxychlor were tested alone and in combination to assess the similarity of their actions on hormone synthesis in gonadal tissue from largemouth bass (Micropterus salmoides floridanus), a species whose reproductive fitness has relevance to ecosystem health in Florida (USA). Gonads were harvested from adult female bass (age, two to three years) during the peak reproductive season (January-May), minced, and incubated in culture medium with or without test agents for 48 h. Duplicates of each treatment were performed in each of three experiments using tissue from a different female. Both 17beta-estradiol and testosterone were measured in aliquots of culture medium by validated radioimmunoassay procedures. Dose-response relationships of individual agents were characterized over a 6-log concentration range (1 X 10(-2) to 1 X 10(4) ppb). Both DDE and methoxychlor, tested individually, produced a dose-dependent decrease in testosterone levels. 17beta-Estradiol levels were unaffected. Mixtures of the agents were tested at all concentration combinations of 0.01, 1, 100, and 10,000 ppb in culture medium. Statistical tests indicated that of 16 dose combinations tested, 15 were antagonistic, and only 1 was additive based on the Loewe additivity model of no interaction. These results imply that methoxychlor and DDE inhibit testosterone production by different mechanisms in bass ovaries.

  18. Victims Themselves of a Close Encounter: On the Sensory Language and Bass Fiction of Space Ape (In Memoriam

    Directory of Open Access Journals (Sweden)

    tobias c. van Veen

    2015-11-01

    Full Text Available This text is written in memoriam to dubstep emcee and poet Space Ape (Stephen Samuel Gordon, b. June 17th, 1970; d. October 2nd, 2014. By his own words, Space Ape arose from the depths of the black Atlantic, on a mission to relieve the “pressure” through bass fiction. My aim is to explicate Space Ape’s bass fiction as the intersection of material and imaginal forces, connecting it to a broader Afrofuturist constellation of mythopoetic becomings. Memory and matter converge in the affect and sounding of Space Ape the “hostile alien” (“Space Ape”, Burial, 2006, a figure shaped at the intersection of the dread body, riddim warfare, and speculative lyricism. Space Ape set out to “xorcise” that which consumed him from within by embracing the “spirit of change”. Turning to process philosophy, I demonstrate how Space Ape’s bass fiction—his virtual body—activates the abstract concepts of becoming in the “close encounter” with the hostile alien.

  19. Species profiles: Life histories and environmental requirements of coastal fishes and invertebrates (South Atlantic): Black sea bass

    Energy Technology Data Exchange (ETDEWEB)

    Mercer, L.P.

    1989-07-01

    Species profiles are literature summaries on the taxonomy, morphology, range, life history, and environmental requirements of coastal aquatic species. They are designed to assist in environmental impact assessment. The black sea bass, Centropristis striata, is an abundant species associated with the inshore sponge-coral habitat in the South Atlantic Bight (Cape Hatteras to Cape Canaveral). It is a protogynous hermaphrodite (each individual is first a female and then a male) that spawns from January to June on the Continental shelf. Juveniles utilize estuaries, as well as offshore areas, for nurseries. It is a slow growing species with a life span of about 10 years. Juveniles and adults are bottom-feeding carnivores. Adults have been collected at temperatures as low as 6 /degree/C but are most abundant at temperatures of 8 to 10 /degree/C and above. Juveniles tolerate lower temperatures and greater salinity ranges than adults. Black sea bass are primarily harvested by the recreational hook and line fishery and the commercial trap fishery. Yield-per-recruit analyses indicate that the harvest of black sea bass is less than the maximum possible due to a combination of high fishing pressure and harvest of small fish. 58 refs., 2 figs., 6 tabs.

  20. Laser capture microdissection of intestinal tissue from sea bass larvae using an optimized RNA integrity assay and validated reference genes.

    Science.gov (United States)

    Schaeck, M; De Spiegelaere, W; De Craene, J; Van den Broeck, W; De Spiegeleer, B; Burvenich, C; Haesebrouck, F; Decostere, A

    2016-01-01

    The increasing demand for a sustainable larviculture has promoted research regarding environmental parameters, diseases and nutrition, intersecting at the mucosal surface of the gastrointestinal tract of fish larvae. The combination of laser capture microdissection (LCM) and gene expression experiments allows cell specific expression profiling. This study aimed at optimizing an LCM protocol for intestinal tissue of sea bass larvae. Furthermore, a 3'/5' integrity assay was developed for LCM samples of fish tissue, comprising low RNA concentrations. Furthermore, reliable reference genes for performing qPCR in larval sea bass gene expression studies were identified, as data normalization is critical in gene expression experiments using RT-qPCR. We demonstrate that a careful optimization of the LCM procedure allows recovery of high quality mRNA from defined cell populations in complex intestinal tissues. According to the geNorm and Normfinder algorithms, ef1a, rpl13a, rps18 and faua were the most stable genes to be implemented as reference genes for an appropriate normalization of intestinal tissue from sea bass across a range of experimental settings. The methodology developed here, offers a rapid and valuable approach to characterize cells/tissues in the intestinal tissue of fish larvae and their changes following pathogen exposure, nutritional/environmental changes, probiotic supplementation or a combination thereof. PMID:26883391

  1. Characterization of Exopolysaccharides Produced by Bacillus cereus and Brachybacterium sp. Isolated from Asian Sea Bass (Lates calcarifer

    Directory of Open Access Journals (Sweden)

    Mohamed Orsod

    2012-09-01

    Full Text Available Aims: EPS extracted from marine bacteria, which associated with Asian sea bass has potential antimicrobial activities.Methodology and Results: Two marine Bacteria were isolated from Asian sea bass (Lates calcarifer obtained from aquaculture farm, located at Johor bahru Malaysia. 16S rRNA analysis for bacteria identity revealed that bacteria ors1 had 99 % identity to Bacillus cereus and ors2 had 96 % identity with Brachybacterium sp. All bacteria shared many similarities and variation in terms of biochemical reactions and microscopic observation. Exopolysaccharides (EPSs were extracted and purified from bacteria as they produced mucous colonies. Average analysis of EPS components showed 50 % carbohydrates, 26 % protein and 24 % fatty acids. The FTIR analysis confirmed the functional groups of the EPS. Screening for antimicrobial activities assays using Kirby-Bauer methods against both grams positive and negative had shown presence of inhibition zones.Conclusion, significance and impact of study: This study recommends that bacteria isolated from Asian sea bass are having antimicrobial activities and could be used as a potential source for the development of marine drugs.

  2. Safety of oxytetracycline (Terramycin TM-100F) administered in feed to hybrid striped bass, walleyes, and yellow perch

    Science.gov (United States)

    Gaikowski, M.P.; Wolf, J.C.; Schleis, S.M.; Gingerich, W.H.

    2003-01-01

    Oxytetracycline (Terramycin TM-100F, a medicated premix containing oxytetracycline at 220 g/kg) is approved in the United States to control certain systemic bacterial diseases of salmon and catfish when fed at a rate of 55-82.5 mg per kilogram of bodyweight per day for 10 d. Although oxytetracycline may also control certain systemic bacterial infections in coolwater or scaled warmwater fish, no safety data for such species are available. Our objective was to determine the safety of oxytetracycline administered in feed at nominal doses of 0, 82.5, 248, or 413 mg??kg-1??d-1 to yellow perch Perca flavescens and hybrid striped bass (striped bass Morone saxatilis x white bass M. chrysops) for 10 d and to walleye Sander vitreus (formerly Stizostedion vitreum) for 20 d. Yellow perch and hybrid striped bass consumed 50% to 100% of the diet, whereas walleye feed consumption was occasionally less than 50% of the diet. Feed or fecal material was present in the gastrointestinal tract of all necropsied walleyes except for one control fish. The single growth effect was that hybrid striped bass offered a nominal dose of 413 mg??kg-1??d-1 were significantly smaller than untreated controls. Oxytetracycline-related histopathological findings were limited to walleyes and were of low severity. The histopathological findings included decreased hematopoietic-lymphopoietic (H&L) tissue in the anterior kidneys, diffuse hyperplasia of the gill filament epithelium, and a decreased prevalence of fish with eosinophilic droplets in their renal tubular epithelial cells. Although the incidence of decreased H&L tissue tended to increase in proportion to oxytetracycline dose, this finding was statistically significant only for fish that received a nominal dose of 413 mg??kg-1??d-1. Given the pathogenicity of the types of bacteria that are controlled by oxytetracycline treatment and the long history of its use in major aquaculture species, the relative risk of the minor oxytetracycline

  3. Aquatic contaminants alter genes involved in neurotransmitter synthesis and gonadotropin release in largemouth bass

    International Nuclear Information System (INIS)

    Many aquatic contaminants potentially affect the central nervous system, however the underlying mechanisms of how toxicants alter normal brain function are not well understood. The objectives of this study were to compare the effects of emerging and prevalent environmental contaminants on the expression of brain transcripts with a role in neurotransmitter synthesis and reproduction. Adult male largemouth bass (Micropterus salmoides) were injected once for a 96 h duration with control (water or oil) or with one of two doses of a single chemical to achieve the following body burdens (μg/g): atrazine (0.3 and 3.0), toxaphene (10 and 100), cadmium (CdCl2) (0.000067 and 0.00067), polychlorinated biphenyl (PCB) 126 (0.25 and 2.5), and phenanthrene (5 and 50). Partial largemouth bass gene segments were cloned for enzymes involved in neurotransmitter (glutamic acid decarboxylase 65, GAD65; tyrosine hydroxylase) and estrogen (brain aromatase; CYP19b) synthesis for real-time PCR assays. In addition, neuropeptides regulating feeding (neuropeptide Y) and reproduction (chicken GnRH-II, cGnRH-II; salmon GnRH, sGnRH) were also investigated. Of the chemicals tested, only cadmium, PCB 126, and phenanthrene showed any significant effects on the genes tested, while atrazine and toxaphene did not. Cadmium (0.000067 μg/g) significantly increased cGnRH-II mRNA while PCB 126 (0.25 μg/g) decreased GAD65 mRNA. Phenanthrene decreased GAD65 and tyrosine hydroxylase mRNA levels at the highest dose (50 μg/g) but increased cGnRH-II mRNA at the lowest dose (5 μg/g). CYP19b, NPY, and sGnRH mRNA levels were unaffected by any of the treatments. A hierarchical clustering dendrogram grouped PCB 126 and phenanthrene more closely than other chemicals with respect to the genes tested. This study demonstrates that brain transcripts important for neurotransmitter synthesis neuroendocrine function are potential targets for emerging and prevalent aquatic contaminants.

  4. Fish silage in black bass (Micropterus Salmoides feed as an alternative to fish meal

    Directory of Open Access Journals (Sweden)

    Lia Ferraz de Arruda

    2009-10-01

    Full Text Available The objective of this study was to use the residues of fermented sardine to elaborate the acid fish silage and its use in feed for aquaculture. Biological assay was performed by feeding largemouth bass (Micropterus salmoides fingerlings (initial weight 22g, with extruded diets (41% crude protein; 3,600 kcal/kg digestible energy containing 0.0, 7.5, 10.0, 12.5, or 15.0% of fish silage in partial substitution to the fish meal. The feed conversion ratio and weight gain for the treatments were: 1.26 and 15.76g; 1.11 and 17.07g; 1.19 and 17.81g; 1.18 and 19.83g; 1.47 and 14.64g, respectively. No significant differences (PO objetivo desta pesquisa foi estudar a utilização de resíduos de sardinha fermentada para elaboração de silagem química e sua utilização em rações para aquacultura. Foi realizado ensaio de ganho de peso utilizando juvenis de "black bass" (Micropterus salmoides, com peso inicial de 22 gramas, onde utilizou-se rações extrusadas (41% de proteína bruta, 3.600 kcal/kg de energia digestível contendo 0,0; 7,5; 10,0; 12,5; 15,0% de silagem em substituição à farinha de peixe. A composição química da silagem de pescado (matéria seca apresentou valores de proteína bruta de 28,47 g 100g-1; lipídeo 8,24 g 100g-1; cinza 60,68 g 100g-1 A conversão alimentar e o ganho de peso para os tratamentos foram, respectivamente: 1,26 e 15,76g; 1,11 e 17,07g; 1,19 e 17,81g; 1,18 e 19,83g; 1,47 e 14,64g. Nenhuma diferença significativa (P<0,05 foi encontrada entre os tratamentos. Os resultados indicam a possibilidade da utilização de até 15% de silagem na substituição da farinha de peixe, em rações para peixes carnívoros.

  5. Aquatic contaminants alter genes involved in neurotransmitter synthesis and gonadotropin release in largemouth bass.

    Science.gov (United States)

    Martyniuk, Christopher J; Sanchez, Brian C; Szabo, Nancy J; Denslow, Nancy D; Sepúlveda, Maria S

    2009-10-19

    Many aquatic contaminants potentially affect the central nervous system, however the underlying mechanisms of how toxicants alter normal brain function are not well understood. The objectives of this study were to compare the effects of emerging and prevalent environmental contaminants on the expression of brain transcripts with a role in neurotransmitter synthesis and reproduction. Adult male largemouth bass (Micropterus salmoides) were injected once for a 96 h duration with control (water or oil) or with one of two doses of a single chemical to achieve the following body burdens (microg/g): atrazine (0.3 and 3.0), toxaphene (10 and 100), cadmium (CdCl(2)) (0.000067 and 0.00067), polychlorinated biphenyl (PCB) 126 (0.25 and 2.5), and phenanthrene (5 and 50). Partial largemouth bass gene segments were cloned for enzymes involved in neurotransmitter (glutamic acid decarboxylase 65, GAD65; tyrosine hydroxylase) and estrogen (brain aromatase; CYP19b) synthesis for real-time PCR assays. In addition, neuropeptides regulating feeding (neuropeptide Y) and reproduction (chicken GnRH-II, cGnRH-II; salmon GnRH, sGnRH) were also investigated. Of the chemicals tested, only cadmium, PCB 126, and phenanthrene showed any significant effects on the genes tested, while atrazine and toxaphene did not. Cadmium (0.000067 microg/g) significantly increased cGnRH-II mRNA while PCB 126 (0.25 microg/g) decreased GAD65 mRNA. Phenanthrene decreased GAD65 and tyrosine hydroxylase mRNA levels at the highest dose (50 microg/g) but increased cGnRH-II mRNA at the lowest dose (5 microg/g). CYP19b, NPY, and sGnRH mRNA levels were unaffected by any of the treatments. A hierarchical clustering dendrogram grouped PCB 126 and phenanthrene more closely than other chemicals with respect to the genes tested. This study demonstrates that brain transcripts important for neurotransmitter synthesis neuroendocrine function are potential targets for emerging and prevalent aquatic contaminants. PMID:19781795

  6. Aquatic contaminants alter genes involved in neurotransmitter synthesis and gonadotropin release in largemouth bass

    Energy Technology Data Exchange (ETDEWEB)

    Martyniuk, Christopher J. [Department of Physiological Sciences and Center for Environmental and Human Toxicology, University of Florida, Gainesville, FL 32611 (United States); Sanchez, Brian C. [Department of Forestry and Natural Resources and School of Civil Engineering, 195 Marsteller St., Purdue University, West Lafayette, IN 47907 (United States); Szabo, Nancy J.; Denslow, Nancy D. [Department of Physiological Sciences and Center for Environmental and Human Toxicology, University of Florida, Gainesville, FL 32611 (United States); Sepulveda, Maria S., E-mail: mssepulv@purdue.edu [Department of Forestry and Natural Resources and School of Civil Engineering, 195 Marsteller St., Purdue University, West Lafayette, IN 47907 (United States)

    2009-10-19

    Many aquatic contaminants potentially affect the central nervous system, however the underlying mechanisms of how toxicants alter normal brain function are not well understood. The objectives of this study were to compare the effects of emerging and prevalent environmental contaminants on the expression of brain transcripts with a role in neurotransmitter synthesis and reproduction. Adult male largemouth bass (Micropterus salmoides) were injected once for a 96 h duration with control (water or oil) or with one of two doses of a single chemical to achieve the following body burdens ({mu}g/g): atrazine (0.3 and 3.0), toxaphene (10 and 100), cadmium (CdCl{sub 2}) (0.000067 and 0.00067), polychlorinated biphenyl (PCB) 126 (0.25 and 2.5), and phenanthrene (5 and 50). Partial largemouth bass gene segments were cloned for enzymes involved in neurotransmitter (glutamic acid decarboxylase 65, GAD65; tyrosine hydroxylase) and estrogen (brain aromatase; CYP19b) synthesis for real-time PCR assays. In addition, neuropeptides regulating feeding (neuropeptide Y) and reproduction (chicken GnRH-II, cGnRH-II; salmon GnRH, sGnRH) were also investigated. Of the chemicals tested, only cadmium, PCB 126, and phenanthrene showed any significant effects on the genes tested, while atrazine and toxaphene did not. Cadmium (0.000067 {mu}g/g) significantly increased cGnRH-II mRNA while PCB 126 (0.25 {mu}g/g) decreased GAD65 mRNA. Phenanthrene decreased GAD65 and tyrosine hydroxylase mRNA levels at the highest dose (50 {mu}g/g) but increased cGnRH-II mRNA at the lowest dose (5 {mu}g/g). CYP19b, NPY, and sGnRH mRNA levels were unaffected by any of the treatments. A hierarchical clustering dendrogram grouped PCB 126 and phenanthrene more closely than other chemicals with respect to the genes tested. This study demonstrates that brain transcripts important for neurotransmitter synthesis neuroendocrine function are potential targets for emerging and prevalent aquatic contaminants.

  7. An evaluation of biomarkers of reproductive function and potential contaminant effects in Florida largemouth bass (Micropterus salmoides floridanus) sampled from the St. Johns River

    Science.gov (United States)

    Sepulveda, M.S.; Johnson, W.E.; Higman, J.C.; Denslow, N.D.; Schoeb, T.R.; Gross, T.S.

    2002-01-01

    The objective of this study was to describe and compare several reproductive parameters for Florida largemouth bass (Micropterus salmoides floridanus) inhabiting the St. Johns River and exposed to different types and/or degrees of contamination. Welaka was selected as the reference site in this study because of its low urban and agricultural development, Palatka is in close proximity to a paper mill plant, the Green Cove site is influenced by marine shipping activities and Julington Creek site receives discharges of domestic wastewater and storm water runoff from recreational boating marinas. For this study, bass were sampled both prior to (September 1996) and during the spawning season (February 1997). In order to characterize chemical exposure, bass livers were analyzed for up to 90 trace organics and 11 trace metal contaminants. Reproductive parameters measured included gonadosomatic index (GSI), histological evaluation of gonads and plasma concentrations of vitellogenin (VTG), 17??-estradiol (E2) and 11-ketotestosterone (11-KT). In general, the sum of organic chemicals was highest in livers from Palatka bass and bass from Green Cove and Julington Creek had higher hepatic concentrations of low molecular polycyclic aromatic hydrocarbons and polychlorinated biphenyls when compared to fish from Welaka. Metals were more variable across sites, with highest mean concentrations found in bass from either Julington Creek (Ag, As, Cr, Cu, Zn) or Welaka (Cd, Hg, Pb, Se, Tn). Female bass from Palatka and Green Cove had lower concentrations of E2, VTG and lower GSI in relation to Welaka. Males from Palatka and Green Cove showed comparable declines in 11-KT in relation to males from Julington Creek and GSI were decreased only in Palatka males. These results indicate a geographical trend in reproductive effects, with changes being most pronounced at the site closest to the paper mill (Palatka) and decreasing as the St. Johns River flows downstream. Since reproductive

  8. Is the perceived resiliency of fish larvae to ocean acidification masking more subtle effects?

    Directory of Open Access Journals (Sweden)

    E. C. Pope

    2013-10-01

    Full Text Available Ocean acidification, caused by rising concentrations of carbon dioxide (CO2, is widely considered to be a major global threat to marine ecosystems. To investigate the potential effects of ocean acidification on the early life stages of a commercially important fish species, European sea bass (Dicentrarchus labrax, 12 000 larvae were incubated from hatch through metamorphosis under a matrix of two temperatures (17 and 19 °C and two seawater pCO2s (400 and 750 μatm and sampled regularly for 42 days. Calculated daily mortality was significantly affected by both temperature and pCO2, with both increased temperature and elevated pCO2 associated with lower daily mortality and a significant interaction between these two factors. There was no significant pCO2 effect noted on larval morphology during this period but larvae raised at 19 °C possessed significantly larger eyes and lower carbon:nitrogen ratios at the end of the study compared to those raised under 17 °C. These results suggest that D. labrax larvae are resilient to near-future oceanic conditions. However, when the incubation was continued to post-metamorphic (juvenile animals (day 67–69, fish raised under a combination of 19 °C and 750 μatm pCO2 were significantly heavier and exhibited lower aerobic scopes than those incubated at 19 °C and 400 μatm. Most other studies investigating the effects of near-future oceanic conditions on the early life stages of marine fish have used incubations of relatively short durations and suggested these animals are resilient to ocean acidification. We propose the durations of these other studies may be insufficient for more subtle effects, such as those observed in this study, to become apparent. These findings may have important implications for both sea bass in a changing ocean and also for the interpretation of results from other studies that have shown resiliency in marine teleosts exposed to higher atmospheric concentrations of CO2.

  9. Mycobacteriosis in wild rabbitfish Siganus rivulatus associated with cage farming in the Gulf of Eilat, Red Sea.

    Science.gov (United States)

    Diamant, A; Banet, A; Ucko, M; Colorni, A; Knibb, W; Kvitt, H

    2000-02-01

    Infection patterns of Mycobacterium marinum were studied over a period of 3 yr in wild rabbitfish Siganus nivulatus populations associated with commercial mariculture cages and inhabiting various sites along the Israeli Red Sea coastline. Mycobacteriosis was first recorded from the Red Sea in 1990 in farmed sea bass Dicentrarchus labrax and is absent from records of studies on parasites and diseases of wild rabbitfish carried out in the 1970s and 1980s. A sharp increase in the prevalence of the disease in cultured and wild fish in the region has occurred since. A total of 1142 rabbitfish were examined over a 3 yr period from inside mariculture net cages, from the cage surroundings and from several sites along the coast. Histological sections of spleens were examined for presence of granulomatous lesions. Overall prevalence levels of 50% were recorded in the rabbitfish sampled inside the net cages and 39% at the cages' close surroundings, 21% at a sandy beach site 1.2 km westwards, 35% at Eilat harbour 3 km to the south and 42% at a coral reef site about 10 km south of the cages. In addition, 147 fish belonging to 18 native Red Sea species were sampled from 2 sites, the net cage farm perimeter and the coral reef area, and examined for similar lesions. None of those from the coral reef were infected with Mycobacterium; however, 9 of 14 species collected from the cage surroundings were infected. An increase in prevalence of mycobacteriosis in the mariculture farm area was noted from 1995 to 1997. At the same time, a significant increase in prevalence was also apparent at the coral reef sampling site. Two M. marinum isolates from rabbitfish captured at Eilat harbour and the coral reef site were shown by 16S rDNA sequencing analysis to be identical to isolates from rabbitfish trapped inside the mariculture cages as well as isolates from locally cultured sea bass D. labrax. The implications of spreading of M. marinum infection in wild fish populations in the Gulf of

  10. Individual-based model of young-of-the-year striped bass population dynamics. II. Factors affecting recruitment in the Potomac River, Maryland

    Energy Technology Data Exchange (ETDEWEB)

    Cowan, J.H. (Univ. of South Alabama, Mobile, AL (United States)); Rose, K.A. (Oak Ridge National Lab., TN (United States)); Rutherford, E.S.; Houde, E.D. (Univ. of Maryland System, Solomons, MD (United States))

    1993-05-01

    An individual-based model of the population dynamics of young-of-the-year striped bass Morone saxatilis in the Potomac River, Maryland, was used to test the hypothesis that historically high recruitment variability can be explained by changes in environmental and biological factors that result in relatively small changes in growth and mortality rates of striped bass larvae. The four factors examined were (1) size distribution of female parents, (2) zooplankton prey density during the development of striped bass larvae, (3) density of completing larval white perch M. americana, and (4) temperature during larval development. Simulation results suggest that variations in female size and in prey for larvae alone could cause 10-fold variability in recruitment. But no single factor alone caused changes in vital rates of age-0 fish that could account for the 145-fold variability in the Potomac River index of juvenile recruitment. However, combined positive or negative effects of two or more factors resulted in more than a 150-fold simulated recruitment variability, suggesting that combinations of factors can account for the high observed annual variability in striped bass recruitment success. Higher cumulative mortality of feeding larvae and younger life stages than of juveniles was common to all simulations. supporting the contention that striped bass year-class strength is determined prior to metamorphosis. 76 refs., 7 figs., 4 tabs.

  11. Chemical contamination and the annual summer die-off of striped bass (Morone saxatilis) in the Sacramento-San Joaquin Delta.

    Science.gov (United States)

    Cashman, J R; Maltby, D A; Nishioka, R S; Bern, H A; Gee, S J; Hammock, B D

    1992-01-01

    In 1987, striped bass (Morone saxatilis) that were nearly dead (moribund) were captured by hand net, and apparently healthy striped bass were caught by hook and line from adjacent waters in the Sacramento-San Joaquin Delta or, alternatively, caught by hook and line from the Pacific Ocean. The livers of these three groups of striped bass were examined for chemical contamination by gas chromatography, by gas chromatography-mass spectrometry, and by immunoassay. Moribund striped bass livers were greatly contaminated by chemicals compared to healthy fish caught in the Delta and the Pacific Ocean. The types of contaminant encountered suggested that industrial, agricultural, and urban pollutants were present in the livers of moribund fish. Although the variability in the amount of hepatic contaminants observed among the groups of fish does not provide direct proof of causation, the large amount of pollutants suggests that chemical contamination (possibly acting as multiple stressors) contributes to the hepatotoxic condition of the moribund striped bass and may lead to an explanation of the die-off in the Sacramento-San Joaquin Delta region.

  12. Dietary exposure of largemouth bass to OCPs changes expression of genes important for reproduction

    Science.gov (United States)

    Garcia-Reyero, Natalia; Barber, D.S.; Gross, T.S.; Johnson, K.G.; Sepulveda, M.S.; Szabo, N.J.; Denslow, N.D.

    2006-01-01

    Dieldrin and p,p???-DDE are ubiquitous contaminants known to act as endocrine disruptors, causing impaired development and reproduction in fish and wildlife. In order to elucidate the mechanisms by which dieldrin and p,p???-DDE cause endocrine disruption in largemouth bass (Micropterus salmoides), fish were exposed subchronically through the diet to both contaminants. Following 120 days of exposure, p,p???-DDE decreased estradiol in females, but increased 11-ketotestosterone in both sexes. Dieldrin on the other hand, decreased estradiol and 11-ketotestosterone in both sexes. Both pesticides also altered steady state mRNA expression levels of a set of genes chosen to represent three possible mechanisms of endocrine disruption: (1) direct interaction with soluble sex steroid receptors, (2) biosynthesis of endogenous sex hormones, and (3) metabolism of endogenous hormones. p,p???-DDE acted as a weak estrogen, increasing the expression of vitellogenin and estrogen receptor ?? in the liver. p,p???-DDE also altered the expression of genes involved in the synthesis of endogenous hormones as well as their metabolism. Dieldrin, on the other hand, only altered expression of vitellogenin and not estrogen receptor ??. Dieldrin also altered the expression of genes involved in hormone synthesis and metabolism, and it dramatically lowered plasma hormone levels. Both pesticides targeted expression of genes involved in all three modes of action, suggesting that they each have multiple modes of action. ?? 2006 Elsevier B.V. All rights reserved.

  13. Structure and Specificity of a Binary Tandem Domain F-Lectin from Striped Bass (Morone saxatilis)

    Energy Technology Data Exchange (ETDEWEB)

    Bianchet, M.; Odom, E; Vasta, J; Amzel, M

    2010-01-01

    The plasma of the striped bass Morone saxatilis contains a fucose-specific lectin (MsaFBP32) that consists of two F-type carbohydrate recognition domains (CRDs) in tandem. The crystal structure of the complex of MsaFBP32 with l-fucose reported here shows a cylindrical 81-A-long and 60-A-wide trimer divided into two globular halves: one containing N-terminal CRDs (N-CRDs) and the other containing C-terminal CRDs (C-CRDs). The resulting binding surfaces at the opposite ends of the cylindrical trimer have the potential to cross-link cell surface or humoral carbohydrate ligands. The N-CRDs and C-CRDs of MsaFBP32 exhibit significant structural differences, suggesting that they recognize different glycans. Analysis of the carbohydrate binding sites provides the structural basis for the observed specificity of MsaFBP32 for simple carbohydrates and suggests that the N-CRD recognizes more complex fucosylated oligosaccharides and with a relatively higher avidity than the C-CRD. Modeling of MsaFBP32 complexed with fucosylated glycans that are widely distributed in prokaryotes and eukaryotes rationalizes the observation that binary tandem CRD F-type lectins function as opsonins by cross-linking 'non-self' carbohydrate ligands and 'self' carbohydrate ligands, such as sugar structures displayed by microbial pathogens and glycans on the surface of phagocytic cells from the host.

  14. Modeling the effects of potential salinity shifts on the recovery of striped bass in the Savannah River estuary, Georgia-South Carolina, United States

    Science.gov (United States)

    Reinert, T.R.; Peterson, J.T.

    2008-01-01

    Increased salinity in spawning and nursery grounds in the Savannah River estuary was cited as the primary cause of a 97% decrease in adult striped bass (Morone saxatilis) and a concomitant 96% decrease in striped bass egg production. Restoration efforts focused on environmental remediation and stock enhancement have resulted in restored salinity patterns and increased egg and adult abundances. However, future water needs or harbor development may preclude further recovery by reducing freshwater inflow or increasing salinity intrusion. To assess the effect of potential changes in the salinity regime, we developed models relating discharge, tidal phase, and salinity to striped bass egg and early larval survival and re-cast these in a quantitative Bayesian belief network. The model indicated that a small upstream shift (???1.67 km) in the salinity regime would have the least impact on striped bass early life history survival, whereas shifts >1.67 km would have progressively larger impacts, with a 8.33-km shift potentially reducing our estimated survival probability by >28%. Such an impact could have cumulative and long-term detrimental effects on the recovery of the Savannah River striped bass population. The available salinity data were collected during average and low flows, so our model represents some typical and some extreme conditions during a striped bass spawning season. Our model is a relatively simplistic, "first-order" attempt at evaluating potential effects of changes in the Savannah River estuarine salinity regime and points to areas of concern and potential future research. ?? 2008 Springer Science+Business Media, LLC.

  15. Potential effects of maternal contribution on egg and larva population dynamics of striped bass: Integrated individual-based model and directed field sampling

    Energy Technology Data Exchange (ETDEWEB)

    Cowan, J.H., Jr. (Maryland Univ., Solomons, MD (United States). Chesapeake Biological Lab.); Rose, K.A. (Oak Ridge National Lab., TN (United States))

    1991-01-01

    We have used a bioenergetically-driven, individual-based model (IBM) of striped bass as a framework for synthesizing available information on population biology and quantifying, in a relative sense, factors that potentially affect year class success. The IBM has been configured to simulate environmental conditions experienced by several striped bass populations; i.e., in the Potomac River, MD; in Hudson River, NY; in the Santee-Cooper River System, SC, and; in the San Joaquin-Sacramento River System CA. These sites represent extremes in the geographic distribution and thus, environmental variability of striped bass spawning. At each location, data describing the physio-chemical and biological characteristics of the spawning population and nursery area are being collected and synthesized by means of a prioritized, directed field sampling program that is organized by the individual-based recruitment model. Here, we employ the striped bass IBM configured for the Potomac River, MD from spawning into the larval period to evaluate the potential for maternal contribution to affect larva survival and growth. Model simulations in which the size distribution and spawning day of females are altered indicate that larva survival is enhanced (3.3-fold increase) when a high fraction of females in the spawning population are large. Larva stage duration also is less ({bar X} = 18.4 d and 22.2 d) when large and small females, respectively, are mothers in simulations. Although inconclusive, these preliminary results for Potomac River striped bass suggest that the effects of female size, timing of spawning nad maternal contribution on recruitment dynamics potentially are important and illustrate our approach to the study of recruitment in striped bass. We hope to use the model, field collections and management alternatives that vary from site to site, in an iterative manner for some time to come. 54 refs., 4 figs., 1 tab.

  16. Improving oral hygiene skills by computer-based training: a randomized controlled comparison of the modified Bass and the Fones techniques.

    Directory of Open Access Journals (Sweden)

    Daniela Harnacke

    Full Text Available BACKGROUND: Gingivitis and other plaque-associated diseases have a high prevalence in western communities even though the majority of adults report daily oral hygiene. This indicates a lack of oral hygiene skills. Currently, there is no clear evidence as to which brushing technique would bring about the best oral hygiene skills. While the modified Bass technique is often recommended by dentists and in textbooks, the Fones technique is often recommended in patient brochures. Still, standardized comparisons of the effectiveness of teaching these techniques are lacking. METHODOLOGY/PRINCIPAL FINDINGS: In a final sample of n = 56 students, this multidisciplinary, randomized, examiner-blinded, controlled study compared the effects of parallel and standardized interactive computer presentations teaching either the Fones or the modified Bass technique. A control group was taught the basics of tooth brushing alone. Oral hygiene skills (remaining plaque after thorough oral hygiene and gingivitis were assessed at baseline and 6, 12, and 28 weeks after the intervention. We found a significant group×time interaction for gingivitis (F(4/102 = 3.267; p = 0.016; ε = 0.957; η(2 = 0.114 and a significant main effect of group for oral hygiene skills (F(2/51 = 7.088; p = 0.002; η(2 = 0.218. Fones was superior to Bass; Bass did not differ from the control group. Group differences were most prominent after 6 and 12 weeks. CONCLUSIONS/SIGNIFICANCE: The present trial indicates an advantage of teaching the Fones as compared to the modified Bass technique with respect to oral hygiene skills and gingivitis. Future studies are needed to analyze whether the disadvantage of teaching the Bass technique observed here is restricted to the teaching method employed. TRIAL REGISTRATION: German Clinical Trials Register DRKS00003488.

  17. Relations between and among contaminant concentrations and biomarkers in black bass (Micropterus spp.) and common carp (Cyprinus carpio) from large U.S. rivers, 1995-2004

    Science.gov (United States)

    Hinck, J.E.; Schmitt, C.J.; Ellersieck, Mark R.; Tillitt, D.E.

    2008-01-01

    Environmental contaminant and biomarker monitoring data from major U.S. river basins were summarized for black bass (Micropterus spp.) and common carp (Cyprinus carpio) sampled over a nine year period. Cumulative frequency distributions revealed taxon differences for many organochlorine residue concentrations, elemental contaminant concentrations, and biomarkers, but few gender differences were evident for chemical concentrations. Concentrations of dacthal, pentachloroanisole, p,p???-DDE, endosulfan sulfate, barium, cadmium, copper, manganese, lead, selenium, vanadium, and zinc were greater in carp than bass, but concentrations of mercury and magnesium were greater in bass. Gender differences were evident in bass for mercury and in carp for zinc, but the differences were small compared to taxon differences. Greater vitellogenin concentrations, 17??-estradiol concentrations, 17??-estradiol/11-ketotestosterone ratios, and percent oocyte atresia in female carp compared to female bass may be related to the sequential spawning of carp. Regression analyses indicated that as much as 78% of biomarker variation was explained by chemical contaminant concentrations. Sites grouped consistently by river basin in the chemical contaminant principal components analysis (PCA) models and were driven by mercury, magnesium, barium, mirex, and oxychlordane. PCA models for the biomarkers did not group the sites by basin for either bass or carp. Statistical analyses and data interpretation were limited by the study design. The implications of these limitations are discussed. Recommendations to be considered during the planning of future monitoring studies include the exclusion of gender- and species-specific sampling for certain chemical contaminants considering analytical methods with appropriate sensitivities; and allowing for the addition of new chemical and biological variables as methods and information needs evolve. ?? The Royal Society of Chemistry.

  18. Tissue distribution of organochlorine pesticides in largemouth bass (Micropterus salmoides) from laboratory exposure and a contaminated lake.

    Science.gov (United States)

    Dang, Viet D; Kroll, Kevin J; Supowit, Samuel D; Halden, Rolf U; Denslow, Nancy D

    2016-09-01

    Tissue concentrations of persistent organochlorine pesticides in laboratory-exposed largemouth bass (Micropterus salmoides) and in bass collected from Lake Apopka, FL were determined by both total mass and lipid normalized mass to better understand the bioaccumulation pathways of contaminants. In the laboratory study, male bass were orally administered a single dose of a mixture of two pesticides (p,p'-dichlorodiphenyldichloroethylene (p,p'-DDE) and dieldrin) and then fed uncontaminated food for 28 days. Gastrointestinal tract, liver, brain, gonad, kidney, spleen, and muscle were collected for chemical analysis. Different profiles were observed by total contaminant mass in tissues compared to lipid normalized mass. On a lipid normalized basis, p,p'-DDE was highest in the gastrointestinal tract followed by the liver, gonad, spleen, muscle, kidney and then brain. Dieldrin, on the other hand, was highest in the gastrointestinal tract and spleen and then followed by the gonad, muscle, liver, kidney, and brain. Distribution of the chemicals among the organs differed by their log KOW values and generally followed the blood flow path after the gastrointestinal tract. The low contaminant levels found in kidney and brain suggest insufficient time for equilibration into these tissues, especially into the brain where the blood-brain barrier may be slow to traverse. In Lake Apopka fish, dichlorodiphenyltrichloroethanes (DDXs, sum of p,p'-DDE, p,p'-DDD, and p,p'-DDT), Drins (sum of aldrin, dieldrin, and endrin), and hexachlorocyclohexanes (HCHs) were found. For DDXs, the lipid normalized concentrations in each tissue were about the same, as predicted from theory. For Drins and HCHs, the lipid normalized concentrations were similar for kidney, spleen, brain, gonad and muscle, but much lower in the gastrointestinal tract and liver, probably because of metabolism occurring in those tissues. PMID:27394080

  19. Reproductive endocrine disruption in smallmouth bass (Micropterus dolomieu) in the Potomac River basin: spatial and temporal comparisons of biological effects

    Science.gov (United States)

    Blazer, Vicki; Iwanowicz, Luke R.; Henderson, Holly; Mazik, Patricia M.; Jenkins, Jill A.; Alvarez, David A.; Young, John A.

    2012-01-01

    A high prevalence of intersex or testicular oocytes (TO) in male smallmouth bass within the Potomac River drainage has raised concerns as to the health of the river. Studies were conducted to document biomarker responses both temporally and spatially to better understand the influence of normal physiological cycles, as well as water quality and land-use influences. Smallmouth bass were collected over a 2-year period from three tributaries of the Potomac River: the Shenandoah River, the South Branch Potomac and Conococheague Creek, and an out-of-basin reference site on the Gauley River. The prevalence of TO varied seasonally with the lowest prevalence observed in July, post-spawn. Reproductive maturity and/or lack of spawning the previous spring, as well as land-use practices such as application of manure and pesticides, may influence the seasonal observations. Annual, seasonal, and site differences were also observed in the percentage of males with measurable concentrations of plasma vitellogenin, mean concentration of plasma vitellogenin in females, and plasma concentrations of 17β-estradiol and testosterone in both sexes. Bass collected in the South Branch Potomac (moderate to high prevalence of TO) had less sperm per testes mass with a lower percentage of those sperm being motile when compared to those from the Gauley River (low prevalence of TO). An inverse relationship was noted between TO severity and sperm motility. An association between TO severity and wastewater treatment plant flow, percent of agriculture, total number of animal feeding operations, the number of poultry houses, and animal density within the catchment was observed.

  20. Tissue distribution of organochlorine pesticides in largemouth bass (Micropterus salmoides) from laboratory exposure and a contaminated lake.

    Science.gov (United States)

    Dang, Viet D; Kroll, Kevin J; Supowit, Samuel D; Halden, Rolf U; Denslow, Nancy D

    2016-09-01

    Tissue concentrations of persistent organochlorine pesticides in laboratory-exposed largemouth bass (Micropterus salmoides) and in bass collected from Lake Apopka, FL were determined by both total mass and lipid normalized mass to better understand the bioaccumulation pathways of contaminants. In the laboratory study, male bass were orally administered a single dose of a mixture of two pesticides (p,p'-dichlorodiphenyldichloroethylene (p,p'-DDE) and dieldrin) and then fed uncontaminated food for 28 days. Gastrointestinal tract, liver, brain, gonad, kidney, spleen, and muscle were collected for chemical analysis. Different profiles were observed by total contaminant mass in tissues compared to lipid normalized mass. On a lipid normalized basis, p,p'-DDE was highest in the gastrointestinal tract followed by the liver, gonad, spleen, muscle, kidney and then brain. Dieldrin, on the other hand, was highest in the gastrointestinal tract and spleen and then followed by the gonad, muscle, liver, kidney, and brain. Distribution of the chemicals among the organs differed by their log KOW values and generally followed the blood flow path after the gastrointestinal tract. The low contaminant levels found in kidney and brain suggest insufficient time for equilibration into these tissues, especially into the brain where the blood-brain barrier may be slow to traverse. In Lake Apopka fish, dichlorodiphenyltrichloroethanes (DDXs, sum of p,p'-DDE, p,p'-DDD, and p,p'-DDT), Drins (sum of aldrin, dieldrin, and endrin), and hexachlorocyclohexanes (HCHs) were found. For DDXs, the lipid normalized concentrations in each tissue were about the same, as predicted from theory. For Drins and HCHs, the lipid normalized concentrations were similar for kidney, spleen, brain, gonad and muscle, but much lower in the gastrointestinal tract and liver, probably because of metabolism occurring in those tissues.

  1. Assessment of reproductive effects in largemouth bass (Micropterus salmoides) exposed to bleached/unbleached kraft mill effluents

    Science.gov (United States)

    Sepulveda, M.S.; Ruessler, D.S.; Denslow, N.D.; Holm, S.E.; Schoeb, T.R.; Gross, T.S.

    2001-01-01

    This study evaluated the potential effects of different concentrations of bleached/unbleached kraft mill effluent (B/UKME) on several reproductive endpoints in adult largemouth bass (Micropterus salmoides). The kraft mill studied produces a 50/50 mix of bleached/unbleached market pulp with an estimated release of 36 million gal of efffluent/day. Bleaching sequences were C90d10EopHDp and CEHD for softwood (pines) and hardwoods (mainly tupelo, gums, magnolia, and water oaks), respectively. Bass were exposed to different effluent concentrations (0 [controls, exposed to well water], 10, 20, 40, or 80%) for either 28 or 56 days. At the end of each exposure period, fish were euthanized, gonads collected for histological evaluation and determination of gonadosomatic index (GSI), and plasma was analyzed for 17??-estradiol, 11-ketotestosterone, and vitellogenin (VTG). Largemouth bass exposed to B/UKME responded with changes at the biochemical level (decline in sex steroids in both sexes and VTG in females) that were usually translated into tissue/organ-level responses (declines in GSI in both sexes and in ovarian development in females). Although most of these responses occurred after exposing fish to 40% B/UKME concentrations or greater, some were observed after exposures to 20% B/UKME. These threshold concentrations fall within the 60% average yearly concentration of effluent that exists in the stream near the point of discharge (Rice Creek), but are above the <10% effluent concentration present in the St. Johns River. The chemical(s) responsible for such changes as well as their mode(s) of action remain unknown at this time.

  2. The potential use of legume-based diets supplemented with microbial phytase on the growth performance and feed efficiency of sea bass, Lates calcarifer

    OpenAIRE

    Erlinda S. Ganzon-Naret

    2013-01-01

    A feeding trial was conducted for 12 weeks to evaluate the potential use of legume-based diets supplemented with dietary microbial phytase on the growth performance and feed efficiency of juvenile sea bass, Lates calcarifer. Fifteen sea bass juveniles (mean initial weight of 0.96 g and mean initial total length (TL) of 4.2 cm) were stocked at three replicates into each of the twelve 100 L conical fibreglass tanks containing 90 L sea water in a closed recirculating system with filt...

  3. The potential use of legume-based diets supplemented with microbial phytase on the growth performance and feed efficiency of sea bass, Lates calcarifer

    OpenAIRE

    Erlinda S. Ganzon-Naret

    2013-01-01

    A feeding trial was conducted for 12 weeks to evaluate the potential use of legume-based diets supplemented with dietary microbial phytase on the growth performance and feed efficiency of juvenile sea bass, Lates calcarifer. Fifteen sea bass juveniles (mean initial weight of 0.96 g and mean initial total length (TL) of 4.2 cm) were stocked at three replicates into each of the twelve 100 L conical fibreglass tanks containing 90 L sea water in a closed recirculating system with ...

  4. Spectre d'absorption de l'hydrogène atomique à très basse pression.

    OpenAIRE

    Moret-Bailly, Jacques

    2015-01-01

    Sous très basse pression, le spectre de l'hydrogène atomique devient un spectre de quasar. Spectra of quasars result primarily from interactions of natural light with atomic hydrogen. A visible absorption of a sharp and saturated spectral line in a gas requires a low pressure, so a long path without blushing as a cosmological redshift. Burbidge and Karlsson observed that redshifts of quasars result from fundamental redshifts, written 3K and 4K, that cause a shift of absorbed beta and gamma...

  5. Gas chromatography-mass spectrometry characterization of the varnish and glue of an ancient 18th century double bass.

    Science.gov (United States)

    Caruso, Francesco; Orecchio, Santino; Cicero, Maria Grazia; Di Stefano, Cosimo

    2007-04-20

    A GC-MS investigation is conducted on the double bass "Panormus", property of Conservatorio di Musica "Vincenzo Bellini" in Palermo. The most important components of the varnish (fatty acids) and of the glue (proteinaceous amino acids), with which the musical instrument was treated in the past, are determined. The analyses are carried out by prior derivatization of fatty acids by acidic methanol and of amino acids by acidic methanol and trifluoroacetic anhydride (TFAA). Analytes identification is achieved by direct comparison with several reference materials and the use of a digitized library. PMID:17376455

  6. Gas chromatography-mass spectrometry characterization of the varnish and glue of an ancient 18th century double bass.

    Science.gov (United States)

    Caruso, Francesco; Orecchio, Santino; Cicero, Maria Grazia; Di Stefano, Cosimo

    2007-04-20

    A GC-MS investigation is conducted on the double bass "Panormus", property of Conservatorio di Musica "Vincenzo Bellini" in Palermo. The most important components of the varnish (fatty acids) and of the glue (proteinaceous amino acids), with which the musical instrument was treated in the past, are determined. The analyses are carried out by prior derivatization of fatty acids by acidic methanol and of amino acids by acidic methanol and trifluoroacetic anhydride (TFAA). Analytes identification is achieved by direct comparison with several reference materials and the use of a digitized library.

  7. RELATION OF BODY WEIGHT AND FOOD CONSUMPTION TO METABOLIC RATE OF JUVENILE JAPANESE SEA BASS,LATEOLABRAX JAPONICUS

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    The metabolic rate of Japanese sea bass, Lateolabrax japonicus (C & V), was estimated in laboratory at temperature 25.2±0.5℃. The fresh weight of the fish was 4.64-52.28 g (average of 17.81±0.33 g). The routine metabolism was related to body weight by the exponential equation: Rr=14.966W0.74 (r=0.934). The rate of feeding metabolism increased linearly with food consumption. Feeding metabolic rate was 1.8-2.4 times the routine metabolic rate.

  8. A study on the efficiency of EFQM versus Bass method: Evidence from national land and housing organization

    Directory of Open Access Journals (Sweden)

    Abolfazl Danaei

    2013-10-01

    Full Text Available Leadership style plays essential role on the success of organizations. The proposed study of this paper compares the efficiency of leadership study based on EFQM and Bass models in national land and housing organization of Iran. The proposed study of this paper designs a questionnaire and distributes it among all managers on this organization. The study covers all different levels of management including middle and top-level management. The results of the survey indicate that the average efficiency of EFQM model is located in 2.7276

  9. An ovary transcriptome for all maturational stages of the striped bass (Morone saxatilis, a highly advanced perciform fish

    Directory of Open Access Journals (Sweden)

    Reading Benjamin J

    2012-02-01

    Full Text Available Abstract Background The striped bass and its relatives (genus Morone are important fisheries and aquaculture species native to estuaries and rivers of the Atlantic coast and Gulf of Mexico in North America. To open avenues of gene expression research on reproduction and breeding of striped bass, we generated a collection of expressed sequence tags (ESTs from a complementary DNA (cDNA library representative of their ovarian transcriptome. Results Sequences of a total of 230,151 ESTs (51,259,448 bp were acquired by Roche 454 pyrosequencing of cDNA pooled from ovarian tissues obtained at all stages of oocyte growth, at ovulation (eggs, and during preovulatory atresia. Quality filtering of ESTs allowed assembly of 11,208 high-quality contigs ≥ 100 bp, including 2,984 contigs 500 bp or longer (average length 895 bp. Blastx comparisons revealed 5,482 gene orthologues (E-value -3, of which 4,120 (36.7% of total contigs were annotated with Gene Ontology terms (E-value -6. There were 5,726 remaining unknown unique sequences (51.1% of total contigs. All of the high-quality EST sequences are available in the National Center for Biotechnology Information (NCBI Short Read Archive (GenBank: SRX007394. Informative contigs were considered to be abundant if they were assembled from groups of ESTs comprising ≥ 0.15% of the total short read sequences (≥ 345 reads/contig. Approximately 52.5% of these abundant contigs were predicted to have predominant ovary expression through digital differential display in silico comparisons to zebrafish (Danio rerio UniGene orthologues. Over 1,300 Gene Ontology terms from Biological Process classes of Reproduction, Reproductive process, and Developmental process were assigned to this collection of annotated contigs. Conclusions This first large reference sequence database available for the ecologically and economically important temperate basses (genus Morone provides a foundation for gene expression studies in these

  10. The characteristics of vasa gene from Japanese sea bass ( Lateolabrax japonicas) and its response to the external hormones

    Science.gov (United States)

    Chi, Meili; Wen, Haishen; Ni, Meng; Qian, Kun; Zhang, Pei; Chai, Senhao

    2015-08-01

    The RNA helicase Vasa is an important regulator of primordial germ cell development. Its function in mature fish, especially the hormone-related differences in maturing male fish has seldom been documented. In this study, a full length cDNA sequence of the vasa gene was cloned from Japanese sea bass, Lateolabrax japonicas, and it was named jsb-vasa. Homology analysis showed that jsb-vasa was closely related to its teleost homologs. The spatial distribution of jsb-vasa indicated that it was only highly expressed in testis, showing its germ cell-specific expression pattern. During the testicular development cycle, jsb-vasa was highly expressed during early period of spermatogenesis, and reduced when spermatogenesis advanced. In addition, the jsb-vasa gene expression was significantly inhibited at 6 h, 12 h and 24 h after injecting hCG (human chorionic gonadotropin) and GnRHa (Gonadotropin-releasing hormone analogue), indicating that jsb-vasa gene may play an important role in spermatogenesis of Japanese sea bass, and be under the regulation of external sex hormones.

  11. Liver proteome response of largemouth bass (Micropterus salmoides) exposed to several environmental contaminants: Potential insights into biomarker development

    Energy Technology Data Exchange (ETDEWEB)

    Sanchez, Brian C., E-mail: bcsanche@purdue.edu [Department of Forestry and Natural Resources, Purdue University, 195 Marsteller Street, West Lafayette, IN 47907 (United States); Ralston-Hooper, Kimberly J., E-mail: ralstonk@purdue.edu [Department of Forestry and Natural Resources, Purdue University, 195 Marsteller Street, West Lafayette, IN 47907 (United States); Kowalski, Kevin A., E-mail: kowalski@purdue.edu [Bindley Bioscience Center, Purdue Proteomics Facility, Purdue University, 1203 West State Street, West Lafayette, IN 47907 (United States); Dorota Inerowicz, H., E-mail: inerowic@purdue.edu [Bindley Bioscience Center, Purdue Proteomics Facility, Purdue University, 1203 West State Street, West Lafayette, IN 47907 (United States); Adamec, Jiri, E-mail: jadamec@purdue.edu [Bindley Bioscience Center, Purdue Proteomics Facility, Purdue University, 1203 West State Street, West Lafayette, IN 47907 (United States); Sepulveda, Maria S., E-mail: mssepulv@purdue.edu [Department of Forestry and Natural Resources, Purdue University, 195 Marsteller Street, West Lafayette, IN 47907 (United States)

    2009-10-19

    Liver proteome response of largemouth bass (Micropterus salmoides) exposed to environmental contaminants was analyzed to identify novel biomarkers of exposure. Adult male bass were exposed to cadmium chloride (CdCl{sub 2}), atrazine, PCB 126, phenanthrene, or toxaphene via intraperitoneal injection with target body burdens of 0.00067, 3.0, 2.5, 50, and 100 {mu}g/g, respectively. After a 96 h exposure, hepatic proteins were separated with two-dimensional gel electrophoresis and differentially expressed proteins (vs. controls) recognized and identified with MALDI-TOF/TOF mass spectrometry. We identified, 30, 18, eight, 19, and five proteins as differentially expressed within the CdCl{sub 2}, atrazine, PCB 126, phenanthrene, and toxaphene treatments, respectively. Alterations were observed in the expression of proteins associated with cellular ion homeostasis (toxaphene), oxidative stress (phenanthrene, PCB 126), and energy production including glycolysis (CdCl{sub 2}, atrazine) and ATP synthesis (atrazine). This work supports the further evaluation of several of these proteins as biomarkers of contaminant exposure in fish.

  12. BANYAN. VII. A New Population of Young Substellar Candidate Members of Nearby Moving Groups from the BASS Survey

    CERN Document Server

    Gagné, Jonathan; Cruz, Kelle L; Lafrenière, David; Doyon, René; Malo, Lison; Burgasser, Adam J; Naud, Marie-Eve; Artigau, Étienne; Bouchard, Sandie; Gizis, John E; Albert, Loïc

    2015-01-01

    [Abbreviated] We present the results of a near-infrared (NIR) spectroscopic follow-up survey of 182 M4-L7 low-mass stars and brown dwarfs (BDs) from the BANYAN All-Sky Survey (BASS) for candidate members of nearby, young moving groups (YMGs). We confirm signs of low-gravity for 42 new BD discoveries with estimated masses between 8-75 $M_{Jup}$ and identify previously unrecognized signs of low gravity for 24 known BDs. This allows us to refine the fraction of low-gravity dwarfs in the high-probability BASS sample to $\\sim$82%. We use this unique sample of 66 young BDs, supplemented with 22 young BDs from the literature, to construct new empirical NIR absolute magnitude and color sequences for low-gravity BDs. We obtain a spectroscopic confirmation of low-gravity for 2MASS J14252798-3650229, which is a new $\\sim$27 $M_{Jup}$, L4 $\\gamma$ bona fide member of AB Doradus. We identify a total of 19 new low-gravity candidate members of YMGs with estimated masses below 13 $M_{Jup}$, seven of which have kinematically ...

  13. The effect of different oil spill remediation techniques on petroleum hydrocarbon elimination in Australian bass (Macquaria novemaculeata).

    Science.gov (United States)

    Cohen, A M; Nugegoda, D; Gagnon, M M

    2001-02-01

    Petroleum hydrocarbons were investigated in juvenile Australian bass, Macquaria novemaculeata, following exposure to the water accommodated fraction (WAF) of Bass Strait crude oil, chemically dispersed crude oil, and burnt crude oil. Each treatment was administered for 16 days either through the water column or through the diet (amphipod, Allorchestes compressa). Polycyclic aromatic hydrocarbon (PAH) elimination was determined by measuring biliary benzo(a)pyrene (B(a)P) and naphthalene-type metabolites. Biliary PAH-type metabolite concentrations varied with the type of oil spill remediation technique, route of exposure (food versus water), and exposure concentration. Fish exposed to chemically dispersed crude oil via the water exhibited the highest PAH-type biliary metabolite concentrations, relative to fish exposed to other treatments. In fish exposed via the diet, the highest concentration of both types of biliary metabolites also appeared in the dispersed oil-exposed individuals. The results suggest that chemically dispersing oil may have the greatest effect on bioavailability of hydrocarbons, both through waterborne and food chain exposures.

  14. Physiological and content considerations for a second low frequency channel for bass management, subwoofers, and low frequency enhancement (LFE)

    Science.gov (United States)

    Miller, Robert E. (Robin)

    2005-04-01

    Perception of very low frequencies (VLF) below 125 Hz reproduced by large woofers and subwoofers (SW), encompassing 3 octaves of the 10 regarded as audible, has physiological and content aspects. Large room acoustics and vibrato add VLF fluctuations, modulating audible carrier frequencies to >1 Hz. By convention, sounds below 90 Hz produce no interaural cues useful for spatial perception or localization, therefore bass management redirects the VLF range from main channels to a single (monaural) subwoofer channel, even if to more than one subwoofer. Yet subjects claim they hear a difference between a single subwoofer channel and two (stereo bass). If recordings contain spatial VLF content, is it possible physiologically to perceive interaural time/phase difference (ITD/IPD) between 16 and 125 Hz? To what extent does this perception have a lifelike quality; to what extent is it localization? If a first approximation of localization, would binaural SWs allow a higher crossover frequency (smaller satellite speakers)? Reported research supports the Jeffress model of ITD determination in brain structures, and extending the accepted lower frequency limit of IPD. Meanwhile, uncorrelated very low frequencies exist in all tested multi-channel music and movie content. The audibility, recording, and reproduction of uncorrelated VLF are explored in theory and experiments.

  15. Estimated loss of juvenile salmonids to predation by northern squawfish, walleyes, and smallmouth bass in John Day Reservoir, Columbia River

    International Nuclear Information System (INIS)

    The authors estimated the loss of juvenile salmonids Oncorhynchus spp. to predation by northern squawfish Ptychocheilus oregonensis, walleyes Stizostedion vitreum, and smallmouth bass Micropterus dolomieu in John Day Reservoir during 1983-1986. Their estimates were based on measures of daily prey consumption, predator numbers, and numbers of juvenile salmonids entering the reservoir during the April-August period of migration. They estimated the mean annual loss was 2.7 million juvenile salmonids. Northern squawfish were responsible for 78% of the total loss; walleyes accounted for 13% and smallmouth bass for 9%. Twenty-one percent of the loss occurred in a small area immediately below McNary Dam at the head of John Day Reservoir. The authors estimated that the three predator species consumed 14% of all juvenile salmonids that entered the reservoir. Mortality changed by month and increased late in the migration season. Monthly mortality estimates ranged from 7% in June and 61% in August. Mortality from predation was highest for chinook salmon O. tshawytscha, which migrated in July and August. Despite uncertainties in the estimates, it is clear that predation by resident fish predators can easily account for previously explained mortality of out-migrating juvenile salmonids. Alteration of the Columbia River by dams and a decline in the number of salmonids could have increased the fraction of mortality caused by predation over what is was in the past

  16. The effect of the dye Aquashade(c) on water quality, phytoplankton, zooplankton, and sunshine bass fingerling production in fertilized culture ponds

    Science.gov (United States)

    Rooted macrophytes make harvesting fingerling sunshine bass from ponds difficult because they can smother fish or tangle in the seines and increase fish stress by increasing time needed to retrieve the fingerlings from the net. Aquashade® is a dye that is used to reduce macrophyte growth by reducin...

  17. SeaBASS Bio-optical and pigment data collected from 1979-08-22 to 2011-12-14 (NCEI Accession 0086308)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This accession contains SeaWiFS Bio-optical Archive and Storage System (SeaBASS) bio-optical, pigment, and other data collected from 1979-08-22 to 2011-12-14....

  18. Identification of centrarchid hepcidins and evidence that 17β-estradiol disrupts constitutive expression of hepcidin-1 and inducible expression of hepcidin-2 in largemouth bass (Micropterus salmoides)

    Science.gov (United States)

    Robertson, L.S.; Iwanowicz, L.R.; Marranca, J.M.

    2009-01-01

    Hepcidin is a highly conserved antimicrobial peptide and iron-regulatory hormone. Here, we identify two hepcidin genes (hep-1 and hep-2) in largemouth bass (Micropterus salmoides) and smallmouth bass (Micropterus dolomieu). Hepcidin-1 contains a putative ATCUN metal-binding site in the amino-terminus that is missing in hepcidin-2, suggesting that hepcidin-1 may function as an iron-regulatory hormone. Both hepcidins are predominately expressed in the liver of largemouth bass, similar to other fish and mammals. Experimental exposure of pond-raised largemouth bass to 17β-estradiol and/or the bacteria Edwardsiella ictaluri led to distinct changes in expression of hep-1 and hep-2. Estradiol reduced the constitutive expression of hep-1 in the liver. Bacterial exposure induced expression of hep-2, suggesting that hepcidin-2 may have an antimicrobial function, and this induction was abolished by estradiol. To our knowledge, this is the first report of the regulation of hepcidin expression by estradiol in either fish or mammals.

  19. Computer simulation model for the striped bass young-of-the-year population in the Hudson River. [Effects of entrainment and impingement at power plants on population dynamics

    Energy Technology Data Exchange (ETDEWEB)

    Eraslan, A.H.; Van Winkle, W.; Sharp, R.D.; Christensen, S.W.; Goodyear, C.P.; Rush, R.M.; Fulkerson, W.

    1975-09-01

    This report presents a daily transient (tidal-averaged), longitudinally one-dimensional (cross-section-averaged) computer simulation model for the assessment of the entrainment and impingement impacts of power plant operations on young-of-the-year populations of the striped bass, Morone saxatilis, in the Hudson River.

  20. Assessment of Aquaflor (c), copper sulfate and potassium permanganate for control of Aeromonas hydrophila and Flavobacterium columnare infection in sunshine bass, Morone chrysops female x Morone saxatilis male

    Science.gov (United States)

    Two experiments were conducted to assess different therapeutants against a mixed infection of Aeromonas hydrophila and Flavobacterium columnare in sunshine bass (SB) (Morone chrysops female x Morone saxatilis male). Experiment 1 assessed the efficacy of copper sulfate (CuSO4), florfenicol-medicated...

  1. Influence of reproductive status sex hormones and temperature on plasma IGF-I concentrations in sunshine bass (Morone Chrysops X Morone Saxatilis)

    Science.gov (United States)

    Insulin-like growth factor-I (IGF-I) concentrations in male and female sunshine bass (Morone chrysops X Morone saxatilis) were determined in March, early April, and late April in outdoor ponds at a commercial farm. Female fish were always larger than male fish, however plasma IGF-I concentrations w...

  2. The effects of menhaden oil, flaxseed oil and a dairy-yeast prebiotic on growth , health , feed conversion , survival , critical maximum temperature, and body composition of sunshine bass

    Science.gov (United States)

    A 12-week feeding trial was conducted with juvenile "sunshine bass" female Morone chrysops x male M. saxatilis using four diets containing two levels of a dairy-yeast prebiotic (0 or 2%) and either menhaden fish oil or flaxseed oil as a lipid source. The objective was to determine the main and inte...

  3. Effect of contrasting agents on survival, performance, and condition of larval hybrid striped bass Morone chrysops x M. saxatilis in tanks.

    Science.gov (United States)

    Turbidity is important in the tank culture of larval cannibalistic fish. The principal goal of these studies were to characterize the utility and feasibility of select contrasting agents, either algae or inert soil, at improving sunshine bass survival and uniformity of size at time of weaning to an ...

  4. Gene networks and toxicity pathways induced by acute cadmium exposure in adult largemouth bass (Micropterus salmoides)

    Energy Technology Data Exchange (ETDEWEB)

    Mehinto, Alvine C., E-mail: alvinam@sccwrp.org [Southern California Coastal Water Research Project, Costa Mesa, CA 92626 (United States); Department of Physiological Sciences and Center for Environmental and Human Toxicology, University of Florida, Gainesville, FL 32611 (United States); Prucha, Melinda S. [Department of Human Genetics, Yerkes National Primate Research Center, Emory University, Atlanta, GA 30322 (United States); Department of Physiological Sciences and Center for Environmental and Human Toxicology, University of Florida, Gainesville, FL 32611 (United States); Colli-Dula, Reyna C.; Kroll, Kevin J.; Lavelle, Candice M.; Barber, David S. [Department of Physiological Sciences and Center for Environmental and Human Toxicology, University of Florida, Gainesville, FL 32611 (United States); Vulpe, Christopher D. [Department of Nutritional Sciences and Toxicology, University of California, Berkeley, CA 94720 (United States); Denslow, Nancy D. [Department of Physiological Sciences and Center for Environmental and Human Toxicology, University of Florida, Gainesville, FL 32611 (United States)

    2014-07-01

    Highlights: • Low-level acute cadmium exposure elicited tissue-specific gene expression changes. • Molecular initiating events included oxidative stress and disruption of DNA repair. • Metallothionein, a marker of metal exposure, was not significantly affected. • We report effects of cadmium on cholesterol metabolism and steroid synthesis. • Diabetic complications and impaired reproduction are potential adverse outcomes. - Abstract: Cadmium is a heavy metal that can accumulate to toxic levels in the environment leading to detrimental effects in animals and humans including kidney, liver and lung injuries. Using a transcriptomics approach, genes and cellular pathways affected by a low dose of cadmium were investigated. Adult largemouth bass were intraperitoneally injected with 20 μg/kg of cadmium chloride (mean exposure level – 2.6 μg of cadmium per fish) and microarray analyses were conducted in the liver and testis 48 h after injection. Transcriptomic profiles identified in response to cadmium exposure were tissue-specific with the most differential expression changes found in the liver tissues, which also contained much higher levels of cadmium than the testis. Acute exposure to a low dose of cadmium induced oxidative stress response and oxidative damage pathways in the liver. The mRNA levels of antioxidants such as catalase increased and numerous transcripts related to DNA damage and DNA repair were significantly altered. Hepatic mRNA levels of metallothionein, a molecular marker of metal exposure, did not increase significantly after 48 h exposure. Carbohydrate metabolic pathways were also disrupted with hepatic transcripts such as UDP-glucose, pyrophosphorylase 2, and sorbitol dehydrogenase highly induced. Both tissues exhibited a disruption of steroid signaling pathways. In the testis, estrogen receptor beta and transcripts linked to cholesterol metabolism were suppressed. On the contrary, genes involved in cholesterol metabolism were highly

  5. Relationships among choral tone and intensity and the spectra of bass singing voices

    Science.gov (United States)

    Neufeld, Charles Warren

    The purpose of this spectrographic study was to compare mean formant frequencies (F1, F2, F3, F4 , F5) of three vowels---[a], [o], and [i]---as bass singers (N = 36) performed identical musical phrases in an authentic choral music performance environment. Two types of choral tone, a "blended" sound with minimal vibrato and an opera-chorus-like sound with free vibrato, and two levels of intensity, piano and forte, were employed in a fully crossed 2 x 2 x 3 multiple analysis of variance (MANOVA) design. A panel of experts verified the tone type and intensity in the subject trials. MANOVA results revealed significant differences among formant frequencies for the main effects of vowel, intensity, and tone type, and interaction effects for vowel by intensity and intensity by tone type. Numerous significant differences were found among the five formant univariate effects. Contrast analysis led to the retention of the null hypothesis (H0) of no significant difference between tone types in F1, and the rejection of H 0 for F2, F3, F4, and F5. The H0 of no significant difference between intensifies was retained for F3, and rejected for all other formants. H0 was rejected for all formants except F5 for the main effect vowel. The null hypotheses for the three two-way interactions were rejected for all formants except vowel by intensity in F3. H0 was rejected for each of the five formants for the interaction of vowel by intensity by tone type. The influence of tone type was eliminated as a factor in F1. F2 was influenced mostly by vowel. Intensity was subordinate to tone type and vowel in F3. F4, the most unstable of all the formants, was subject to differences throughout all the combined effects. Vowel had little or no effect on F5 in all combined effect analyses. Contrast analyses resulted in numerous other findings. The study outcomes affirm the influence of tongue and mouth shape on vowel stability measured in F1 and F2. Results further indicate that aurally identifiable

  6. Spatial and temporal diet patterns of subadult and small adult striped bass in Massachusetts estuaries: Data, a synthesis, and trends across scales

    Science.gov (United States)

    Ferry, K.H.; Mather, Martha E.

    2012-01-01

    Subadult and small adult (375–475 mm total length) striped bass Morone saxatilis are abundant and represent an important component of the recovered U.S. Atlantic coast stocks. However, little is known about these large aggregations of striped bass during their annual foraging migrations to New England. A quantitative understanding of trends in the diets of subadult and small adult migrants is critical to research and management. Because of the complexity of the Massachusetts coast, we were able to compare diets at multiple spatial, temporal, and taxonomic scales and evaluate which of these provided the greatest insights into the foraging patterns of this size of fish. Specifically, during spring through autumn, we quantified the diets of 797 migratory striped bass collected from 13 Massachusetts estuaries distributed among three geographic regions in two biogeographic provinces. Our data provided three useful results. First, subadult and young adult striped bass ate a season-specific mixture of fish and invertebrates. For example, more juvenile Atlantic herring Clupea harengus were eaten in spring than in summer or autumn, more juvenile Atlantic menhaden Brevoortia tyrannus were eaten in autumn than in spring or summer, amphipods were eaten primarily in the southern biogeographic province, and shrimp Crangon sp. were eaten in all locations and seasons. Second, examining diets by season was essential because of the temporal variability in striped bass prey. Grouping prey by fish and invertebrates revealed the potential for predictable differences in growth across geographic locations and seasons, based on the output from simple bioenergetics simulations. Third, of the three spatial scales examined, region provided the most quantitative and interpretable ecological trends. Our results demonstrate the utility of comparing multiple scales to evaluate the best way to depict diet trends in a migrating predator that seasonally uses different geographic locations.

  7. Reproductive health of bass in the potomac, USA, drainage: Part 1. exploring the effects of proximity to wastewater treatment plant discharge

    Science.gov (United States)

    Iwanowicz, L.R.; Blazer, V.S.; Guy, C.P.; Pinkney, A.E.; Mullcan, J.E.; Alvarezw, D.A.

    2009-01-01

    Abstract-Intersex (specifically, testicular oocytes) has been observed in male smalimouth bass (SMB; Micropterus dolomieu) and other centrarchids in the South Branch of the Potomac River, USA, and forks of the Shenandoah River, USA. during the past five years. This condition often is associated with exposure to estrogenic endocrine-disrupting chemicals in some fish species, but such chemicals and their sources have yet to be identified in the Potomac. In an attempt to better understand the plausible causes of this condition, we investigated the reproductive health of bass sampled up- and downstream of wastewater treatment plant (WWTP) effluent point sources on the Potomac River in Maryland, USA. Smallmouth bass were sampled from the Conococheague Creek and the Monocacy River, and largemouth bass (LMB; Micropterus salmoides) were collected near the Blue Plains WWTP on the mainstem of the Potomac River. Chemical analyses of compounds captured in passive samplers at these locations also were conducted. A high prevalence of intersex (82-l00%) was identified in male SMB at all sites regardless of collection area. A lower prevalence of intersex (23%) was identified in male LMB collected at the Blue Plains site. When up- and downstream fish were compared, significant differences were noted only in fish from the Conococheague. Differences included condition factor, gonadosomatic index, plasma vitellogenin concentration, and estrogen to testosterone ratio. In general, chemicals associated with wastewater effluent, storm-water runoff, and agriculture were more prevalent at the downstream sampling sites. An exception was atrazine and its associated metabolites, which were present in greater concentrations at the upstream sites. It appears that proximity to effluent from WWTPs may influence the reproductive health of bass in the Potomac watershed, but inputs from other sources likely contribute to the widespread, high incidence of testicular oocytes. ?? 2009 SETAC.

  8. A biorobotic model of the suction-feeding system in largemouth bass: the roles of motor program speed and hyoid kinematics.

    Science.gov (United States)

    Kenaley, Christopher P; Lauder, George V

    2016-07-01

    The vast majority of ray-finned fishes capture prey through suction feeding. The basis of this behavior is the generation of subambient pressure through rapid expansion of a highly kinetic skull. Over the last four decades, results from in vivo experiments have elucidated the general relationships between morphological parameters and subambient pressure generation. Until now, however, researchers have been unable to tease apart the discrete contributions of, and complex relationships among, the musculoskeletal elements that support buccal expansion. Fortunately, over the last decade, biorobotic models have gained a foothold in comparative research and show great promise in addressing long-standing questions in vertebrate biomechanics. In this paper, we present BassBot, a biorobotic model of the head of the largemouth bass (Micropterus salmoides). BassBot incorporates a 3D acrylic plastic armature of the neurocranium, maxillary apparatus, lower jaw, hyoid, suspensorium and opercular apparatus. Programming of linear motors permits precise reproduction of live kinematic behaviors including hyoid depression and rotation, premaxillary protrusion, and lateral expansion of the suspensoria. BassBot reproduced faithful kinematic and pressure dynamics relative to live bass. We show that motor program speed has a direct relationship to subambient pressure generation. Like vertebrate muscle, the linear motors that powered kinematics were able to produce larger magnitudes of force at slower velocities and, thus, were able to accelerate linkages more quickly and generate larger magnitudes of subambient pressure. In addition, we demonstrate that disrupting the kinematic behavior of the hyoid interferes with the anterior-to-posterior expansion gradient. This resulted in a significant reduction in subambient pressure generation and pressure impulse of 51% and 64%, respectively. These results reveal the promise biorobotic models have for isolating individual parameters and assessing

  9. Productivity and river flux variability in response to the PETM on Atlantic margin at Bass River, NJ.

    Science.gov (United States)

    Stoll, H.; Shimizu, N.; Savain, R.; Zachos, J.; Ziveri, P.

    2009-04-01

    While the dramatic climate warming of the Paleocene-Eocene Thermal Maximum has been well characterized, changes in the hydrological cycle and the broader biogeochemical feedbacks (weathering, nutrients, productivity) are less well constrained. Here we describe new geochemical results from a coastal section on the midlatitude Atlantic margin of the U.S. at Bass River, NJ. We measured the elemental geochemistry of coccoliths to probe the productivity of these algae in response to the changing nutrient dynamics on the shelf in the time interval preceding and during the PETM. Coccoliths extracted from the siliclastic coastal section at Bass River NJ exhibit exceptionally good preservation and negligible overgrowth compared to typical ocean carbonate-rich sediments. Analysis of individual coccoliths using secondary ion mass spectrometry (SIMS) facilitates reliable trace element measurements in this low-carbonate section. Published sequence stratigraphy and microfossil analysis have revealed several third order sea level cycles in the late Paleocene including a highstand during the PETM. Consequently we extend our paleoproductivity records far below the PETM to characterize this background variability. We recognize a pattern of generally maximum productivity during lowstands and minimal productivity during highstands. Because nutrient concentrations decrease significantly with distance from the coast, highstands reduce productivity by shifting the highest nutrient levels landward, away from the site. This is likely due to greater distance from river sources as well as reduced wave turbulence which mixes nutrients into the photic zone. This general pattern is broken during the PETM, which features high productivity despite a sea level highstand. This anomalous high productivity may reflect enhanced riverine nutrient delivery, and potentially changes in wind strength and mixing intensity. Riverine nutrient delivery could increase with higher precipitation or precipitation

  10. Effects of dietary vitamin C on fish and crustacean larvae

    OpenAIRE

    Merchie, G.; Lavens, P.; Sorgeloos, P.

    1995-01-01

    In order to assess the dietary needs for ascorbic acid (AA) at startfeeding, the AA content in the various live diets currently applied in aquaculture (algae, rotifers, Artemia ) was studied. Application of boosting techniques using ascorbyl palmitate (AP) as the vitamin C source enabled the transfer of elevated levels of bioactive vitamin C (up to 2500µg AA.g DW-1) via the live food chain into larvae of fish (Clarias gariepinus, Dicentrarchus labrax, Scophthalmus maximus), shrimp (Pennaeus v...

  11. L'activite de deplacement et le reperage de la nourriture chez le bar juvenile captif

    OpenAIRE

    Anthouard, M; Paris, J

    1983-01-01

    Movement activity and localization of food were studied dynamically with the objective of establishing the capacities of behavioral adaptation of the young Dicentrarchus labrax faced with a novel situation. The role played by previous rearing conditions was demonstrated, the level of stimulation being a sensitive intervening factor (currents associated with the food). The data show the favourable effects of such treatment, and are in agreement with work carried out on higher vertebrates, whic...

  12. Identification and quantification of suspended solids and their effects in modern marine recirculation systems

    OpenAIRE

    Orellana, Jaime

    2007-01-01

    Die effiziente Entfernung von im Wasser suspendierten, partikulären Feststoffen ist ein Schlüsselfaktor für den erfolgreichen Betrieb von Kreislaufanlagen. In der vorliegenden Arbeit wurden die Feststoffe in einem Kreislaufsystem (Recirculating Aquaculture System, RAS) für Fische (Wolfsbarsch, Dicentrarchus labrax) qualitativ und quantitativ analysiert, wobei insbesondere die Größenverteilung der Feststoffe und die Rückhaltung in den mechanischen Filtereinheiten. Die Untersuchungen dienten de...

  13. Lectins with Potential for Anti-Cancer Therapy

    OpenAIRE

    Tammy Yau; Xiuli Dan; Charlene Cheuk Wing Ng; Tzi Bun Ng

    2015-01-01

    This article reviews lectins of animal and plant origin that induce apoptosis and autophagy of cancer cells and hence possess the potential of being developed into anticancer drugs. Apoptosis-inducing lectins encompass galectins, C-type lectins, annexins, Haliotis discus discus lectin, Polygonatum odoratum lectin, mistletoe lectin, and concanavalin A, fucose-binding Dicentrarchus labrax lectin, and Strongylocentrotus purpuratus lectin, Polygonatum odoratum lectin, and mistletoe lectin, Polygo...

  14. The use of alternative energies in hatcheries: an application of Life Cycle Analysis

    Directory of Open Access Journals (Sweden)

    G. De Blasi

    2010-01-01

    Full Text Available Fish production requires great efforts and energies and causes different impacts/effects on the ecosystems. The development of aquaculture is possible, in the medium and long term, only if this is done responsibly, and if the production is reached from both an economic and environmental point of view. In our study we have worked on fry production using 1.5 g sized Dicentrarchus labrax obtained rearing larvae for 120 days after the hatching of the eggs.

  15. The use of alternative energies in hatcheries: an application of Life Cycle Analysis

    OpenAIRE

    Blasi, G.; N. Iaffaldano; A. Boni; R. Roma; Centoducati, G.

    2010-01-01

    Fish production requires great efforts and energies and causes different impacts/effects on the ecosystems. The development of aquaculture is possible, in the medium and long term, only if this is done responsibly, and if the production is reached from both an economic and environmental point of view. In our study we have worked on fry production using 1.5 g sized Dicentrarchus labrax obtained rearing larvae for 120 days after the hatching of the eggs.

  16. Morphological differences between wild and farmed Mediterranean fish

    OpenAIRE

    Arechavala-Lopez, Pablo; Sanchez-Jerez, Pablo; Bayle-Sempere, Just T.; Sfakianakis, Dimitris G.; Somarakis, Stylianos

    2012-01-01

    Gilthead seabream (Sparus aurata L.) and European seabass (Dicentrarchus labrax L.) are important commercial marine fish species both for aquaculture and fisheries in the Mediterranean. It is known that farmed individuals escape from farm facilities, but the extent of escape events is not easy to report and estimate because of the difficulty to distinguish between wild and farmed individuals. In this study, significant differences provided through morphometry evidence that the cranial and bod...

  17. AcEST: DK956827 [AcEST

    Lifescience Database Archive (English)

    Full Text Available c... 389 e-107 tr|Q6YI33|Q6YI33_DICLA Actin OS=Dicentrarchus labrax PE=2 SV=1 389 e-107 tr|Q6Y8J1|Q6Y8J1_MEG...AM Beta-actin OS=Megalobrama amblycephala PE... 389 e-107 tr|Q6Y250|Q6Y250_PAGMA

  18. The applicability of Bass's model of transformational, transactional, and laissez-faire leadership in the hospital administrative environment.

    Science.gov (United States)

    Spinelli, Robert J

    2006-01-01

    The purpose of this study is to evaluate empirically in the hospital administrative environment the relationship of leadership behaviors to subordinate manager's perceived outcomes, through examination of B. M. Bass's (1985) model of transformational, transactional, and laissez-faire leadership. The author measured leadership orientation and outcome factors through subordinate managers' ratings of hospital CEOs using a questionnaire, which asked: Is there a relationship between the leadership styles of hospital CEOs and subordinate managers' self-reported willingness to exert extra effort, perception of leader effectiveness and satisfaction with their leader? Findings revealed that the relationship between transformational leadership and the outcome factors were stronger and more positive than were the transactional and laissez-faire styles. These findings are consistent with the hierarchal patterns reported and support the universality of the model. PMID:16708688

  19. Deux extraits commentés des Basses Œuvres1 de ‘Abduh Khāl

    OpenAIRE

    Lagrange, Frédéric

    2014-01-01

    Le romancier saoudien ‘Abduh Khāl, dans son roman Tarmī bi‑sharar (Les Basses Œuvres) paru en 2009 et couronné par l’International Prize for Arab Fiction en 2010, tend un miroir peu flatteur au Royaume wahhabite : ce monologue d’un tortionnaire violeur au service d’un mystérieux Maître du Palais dans une Djedda déformée par l’affairisme figurant allégoriquement le régime présente un pays corrompu par sa soumission à l’absolutisme. Deux extraits sont ici commentés, en soulignant les rapports i...

  20. Protection des ions organiques contre les dommages induits a l'ADN par les electrons de basse energie

    Science.gov (United States)

    Dumont, Ariane

    Il a ete demontre que les electrons de basse energie (EBE) peuvent induire des cassures simple brin (CSB) a l'ADN, via la formation d'anions transitoires qui decroissent par attachement dissociatif, ou dans d'autres etats electroniques dissociatifs menant a la fragmentation. Afin d'effectuer une etude complete des effets des electrons de basse energie sur la matiere biologique, il est necessaire de comprendre leur mecanismes d'interaction non seulement avec l'ADN, mais avec les constituants de son environnement. Les histones sont une composante importante de l'environnement moleculaire de l'ADN. Leur charge positive leur permet de s'associer aux groupements phosphate anionique de l'ADN. Le role principal de ces proteines basiques consiste a organiser l'ADN et l'empaqueter afin de former la chromatine. Les cations sont une autre composante importante de la cellule; ils jouent un role dans la stabilisation de la conformation B de l'ADN in vitro par leurs interactions avec les petits et grands sillons de l'ADN, ainsi qu'avec le groupement phosphate charge negativement. Avec les histones, ils participent egalement a la compaction de l'ADN pour former la chromatine. Cette etude a pour but de comprendre comment la presence d'ions organiques (sous forme de Tris et d'EDTA) a proximite de l'ADN modifie le rendement de cassures simple brin induit par les electrons de basse energie. Le Tris et l'EDTA ont-ete choisis comme objet d'etude, puisqu'en solution, ils forment le tampon standard pour solubiliser l'ADN dans les experiences in vitro (10mM Tris, 1mM EDTA). De plus, la molecule Tris possede un groupement amine alors que l'EDTA possede 4 groupements carboxyliques. Ensembles, ils peuvent se comporter comme un modele simple pour les acides amines. Le ratio molaire de 10 :1 de Tris par rapport a l'EDTA a pour but d'imiter le comportement des histones qui sont riches en arginine et lysine, acides amines possedant un groupement amine charge positivement additionnel. Des films d

  1. Les nouveaux colzas à basse teneur en glucosilonates : composition chimique et valeur nutritive des tourteaux et graines pour le porc

    OpenAIRE

    Bourdon, D.

    1989-01-01

    Les données de valeur nutritive (valeurs énergétique et azotée) de 30 échantillons de tourteaux et graines de colza à haute (HG) et basse (BG) teneur en glucosinolates, obtenues par mesure de digestibilité directe sur porc à l’engrais, sont présentées par type de tourteau (extrait au solvant, de pression, normal et dépelliculé), de même que pour les graines traitées. Nos résultats expérimentaux sont comparés à un nombre équivalent de données bibliographiques disponibles. Ils permettent ...

  2. Dworshak Dam Impact Assessment and Fishery Investigation and Trout, Bass and Forage Species: Combined Project Completion Report.

    Energy Technology Data Exchange (ETDEWEB)

    Maiolie, Melo; Statler, David P.; Elam, Steve

    1992-10-01

    The Nez Perce Tribe (NPT) and the Idaho Department of Fish and Game (IDFG) entered into separate intergovernmental agreements with the Bonneville Power Administration in a cooperative four-year effort to study impacts of Dworshak Dam operation on resident fisheries. The NPT Department of Fisheries Management focused on rainbow trout, smallmouth bass and forage fish. The IDFG's segment of the project was to document kokanee population dynamics, relate it to the changing nutrient status of the reservoir, evaluate kokanee losses through Dworshak Dam, and make kokanee management recommendations. This final report includes findings for 1990 and 1991 and relates these data to information previously presented in annual reports for 1987, 1988 and 1989.

  3. Aquatic Francisella-like bacterium associated with mortality of intensively cultured hybrid striped bass Morone chrysops x M. saxatilis.

    Science.gov (United States)

    Ostland, V E; Stannard, J A; Creek, J J; Hedrick, R P; Ferguson, H W; Carlberg, J M; Westerman, M E

    2006-10-17

    The present study identifies an emerging disease associated with an aquatic Francisella-like bacterium that can cause mortality in hybrid striped bass Morone chrysops x M. saxatilis reared intensively in freshwater. Clinically affected fish were lethargic, had scattered haemorrhagic cutaneous lesions and diffuse gill pallor. The head kidney and spleen were markedly swollen and contained numerous interstitial granulomas; histological examination revealed small, pleomorphic Gram-negative coccobacilli within vacuolated cells. The bacterium could not be cultured from head kidney homogenates either with standard or enriched microbiological media or following inoculation of a Chinook salmon embryo (CHSE)-214 cell line. No amplification product was obtained from head kidney DNA by polymerase chain reaction (PCR) assay using Piscirickettsia salmonis-specific primers. PCR analysis of infected head kidney homogenate with primers designed for the eubacterial 16S rRNA produced a single amplicon. Phylogenetic analysis of this DNA sequence demonstrated that the sequence aligned most closely with members of the genus Francisella, identified from tilapia Oreochromis spp. in Taiwan and an aquatic Francisella species that was recently isolated from the three-line grunt Parapristipoma trilineatum in Japan. This Francisella-like disease was transmitted to naive hybrid striped bass fingerlings by intraperitoneal injection of tissue homogenates prepared from a natural outbreak. All fish developed gross and histological lesions identical to those from natural outbreaks. Intracellular Gram-negative bacteria were observed within the cytoplasm of cells (presumably macrophages) within the granulomas, but bacteria were not recovered. The 16S DNA sequence of the bacterium obtained from tissues of experimentally infected fish was identical to that obtained from the fish used as infected donor tissue. PMID:17140136

  4. Selenium and other elements in juvenile striped bass from the San Joaquin Valley and San Francisco Estuary, California

    Science.gov (United States)

    Saiki, Michael K.; Palawski, Donald U.

    1990-01-01

    Concentrations of selenium and other trace elements were determined in 55 whole body samples of juvenile anadromous striped bass (Morone saxatilis) from the San Joaquin Valley and San Francisco Estuary, California. The fish (≤1 yr old—the predominant life stage in the San Joaquin Valley) were collected in September–December 1986 from 19 sites in the Valley and 3 sites in the Estuary, and analyzed for the following elements: aluminum (Al), arsenic (As), boron (B), barium (Ba), beryllium (Be), cadmium (Cd), chromium (Cr), copper (Cu), iron (Fe), mercury (Hg), magnesium (Mg), molybdenum (Mo), nickel (Ni), lead (Pb), selenium (Se), strontium (Sr), vanadium (V), and zinc (Zn). When compared to concentrations in whole freshwater fish measured by surveys from other waters, a few samples contained higher levels, of As, Cd, Cu, Pb, and Se. The median concentrations of Al, As, Cu, Fe, Mg, Se, and Sr also differed significantly (P⩽0.05) among sites. However, only Se concentrations were highest (up to 7.9 μg/g dry weight) in samples from Valley sites exposed to agricultural subsurface (tile) drainwater; concentrations were lower in samples collected elsewhere. Water quality variables—especially those strongly influenced by tile drainwater (conductivity, total dissolved solids, total alkalinity, and total hardness)—were also significantly correlated (P⩽0.05) with Se concentrations in fish. Selenium concentrations in striped bass from the Estuary were only one-fourth to one-half the concentrations measured in the most contaminated fish from the San Joaquin River.

  5. Cloning, tissue distribution and effects of fasting on pituitary adenylate cyclase-activating polypeptide in largemouth bass

    Science.gov (United States)

    Li, Shengjie; Han, Linqiang; Bai, Junjie; Ma, Dongmei; Quan, Yingchun; Fan, Jiajia; Jiang, Peng; Yu, Lingyun

    2015-03-01

    Pituitary adenylate cyclase activating polypeptide (PACAP) has a wide range of biological functions. We cloned the full-length cDNAs encoding PACAP and PACAP-related peptide (PRP) from the brain of largemouth bass ( Micropterus salmoides) and used real-time quantitative PCR to detect PRP-PACAP mRNA expression. The PRP-PACAP cDNA has two variants expressed via alternative splicing: a long form, which encodes both PRP and PACAP, and a short form, which encodes only PACAP. Sequence analysis results are consistent with a higher conservation of PACAP than PRP peptide sequences. The expression of PACAP-long and PACAP-short transcripts was highest in the forebrain, followed by the medulla, midbrain, pituitary, stomach, cerebellum, intestine, and kidney; however, these transcripts were either absent or were weakly expressed in the muscle, spleen, gill, heart, fatty tissue, and liver. The level of PACAP-short transcript expression was significantly higher than expression of the long transcript in the forebrain, cerebella, pituitary and intestine, but lower than that of the long transcript in the stomach. PACAP-long and PACAP-short transcripts were first detected at the blastula stage of embryogenesis, and the level of expression increased markedly between the muscular contraction stage and 3 d post hatch (dph). The expression of PACAP-long and PACAP-short transcripts decreased significantly in the brain following 4 d fasting compared with the control diet group. The down-regulation effect was enhanced as fasting continued. Conversely, expression levels increased significantly after 3 d of re-feeding. Our results suggest that PRP-PACAP acts as an important factor in appetite regulation in largemouth bass.

  6. De Novo Transcriptome Analysis of Differential Functional Gene Expression in Largemouth Bass (Micropterus salmoides) after Challenge with Nocardia seriolae

    Science.gov (United States)

    Byadgi, Omkar; Chen, Chi-Wen; Wang, Pei-Chyi; Tsai, Ming-An; Chen, Shih-Chu

    2016-01-01

    Largemouth bass (Micropterus salmoides) are common hosts of an epizootic bacterial infection by Nocardia seriolae. We conducted transcriptome profiling of M. salmoides to understand the host immune response to N. seriolae infection, using the Illumina sequencing platform. De novo assembly of paired-end reads yielded 47,881 unigenes, the total length, average length, N50, and GC content of which were 49,734,288, 1038, 1983 bp, and 45.94%, respectively. Annotation was performed by comparison against non-redundant protein sequence (NR), non-redundant nucleotide (NT), Swiss-Prot, Clusters of Orthologous Groups (COG), Kyoto Encyclopaedia of Genes and Genomes (KEGG), Gene Ontology (GO), and Interpro databases, yielding 28,964 (NR: 60.49%), 36,686 (NT: 76.62%), 24,830 (Swissprot: 51.86%), 8913 (COG: 18.61%), 20,329 (KEGG: 42.46%), 835 (GO: 1.74%), and 22,194 (Interpro: 46.35%) unigenes. Additionally, 8913 unigenes were classified into 25 Clusters of Orthologous Groups (KOGs) categories, and 20,329 unigenes were assigned to 244 specific signalling pathways. RNA-Seq by Expectation Maximization (RSEM) and PossionDis were used to determine significantly differentially expressed genes (False Discovery Rate (FDR) < 0.05) and we found that 1384 were upregulated genes and 1542 were downregulated genes, and further confirmed their regulations using reverse transcription quantitative PCR (RT-qPCR). Altogether, these results provide information on immune mechanisms induced during bacterial infection in largemouth bass, which may facilitate the prevention of nocardiosis. PMID:27529219

  7. VACCINATION OF HYBRID STRIPED BASS: GROWTH, IMMUNE REACTION AND GENE EXPRESSION

    Directory of Open Access Journals (Sweden)

    Mark Westerman

    2012-10-01

    Full Text Available Hybrid striped bass (42.6+4.9 g wet wt; 139.3+6.1 mm length, were randomly stocked into one of 6 tanks (n=6 tank-1 of a custom designed, recirculating life support system (RLSS. Water quality was as follows: DO2 (6.50.6 mg l-1, pH (7.70.5, TAN (0.06-1.31 mg l-1, nitrite (0.06-0.60 mg l-1 and nitrate (2.0-32.1 mg l-1, salinity 5 ppt, temperature 28±1 oC. A 12:12 photophase:scotophase was used, with a 30 min. dusk-dawn dimming of lights. Fish were fed at 4% body wt d-1 as two separate feedings (08.00 and 16.00 h. Dietary crude protein and lipid levels were 40% and 10% respectively. Tanks were randomly paired and fish either left untreated, vaccinated, or sham injected. The vaccine employed was an experimental formalin killed Streptococcus iniae oil-in-water adjuvanted bacterin. Fish were weighed and measured bimonthly for 8 wk with group weights being employed to adjust feeding rates. At trial termination, all animals were weighed and measured, and their condition factor (CF and feed conversion ratios (FCR calculated. Visceral somatic (VSI and hepatosomatic (HSI indices and intraperitoneal fat (IPF and muscle ratios (MR were also assessed. Blood was taken from anaesthetized fish and hematocrit recorded. Blood was allowed to clot overnight at 5 oC after which serum protein levels were recorded and lysozyme activity measured. Livers were prepared for microarray evaluation using standard techniques using a Danio rerio genechip to assess global changes in gene expression. No differences were observed with respect to final weights, lengths, CF, FCR, or HSI although differences (P < 0.001 were determined for VSI, which was higher in control animals. Packed cell volume and serum protein levels were similar across groups (P > 0.05. Time-course of changes in serum lysozyme activity described an initial reduction in lysozyme activity followed by a rebounding in activity which peaked 25 days post-treatments. Evaluation of lysozyme activity among time

  8. Adrenocortical and adrenomedullary homologs in eight species of adult and developing teleosts: morphology, histology, and immunohistochemistry.

    Science.gov (United States)

    Grassi Milano, E; Basari, F; Chimenti, C

    1997-12-01

    Morphology, histology, and immunohistochemistry of the adrenocortical and adrenomedullary homologs (adrenal glands) of the following developing and adult teleosts were examined: Salmoniformes-Oncorhynchus mykiss (rainbow trout), Salmo trutta fario (brown trout), Coregonus lavaretus (white fish); Cyprinodontiformes-Gambusia affinis (mosquito fish). Perciformes-Dicentrarchus labrax (sea bass), Sparus aurata (sea bream), Diplodus sargus (white bream), Oblada melanura (saddled bream). The anatomical relationships of the gland with the renal system and venous vessels were also noted. In adults of all species steroidogenic and catecholaminergic chromaffin cells were found in the head kidney, which is pronephric in origin and subsequently transformed into a hematopoietic lymphatic organ. In Perciformes, chromaffin cells are distributed around the anterior and posterior cardinal veins and ducts of Cuvier; in Salmoniformes, around the posterior cardinal veins and in the hematopoietic tissue; and in G. affinis, around the ducts of Cuvier and posterior cardinal veins, while a few are visible also around the sinus venosus. In Perciformes and Salmoniformes, numerous chromaffin cells are also present in the posterior kidney, derived from the opisthonephros, in contact with the caudal vein. Steroidogenic cells are always confined to the head kidney. During development chromaffin and steroidogenic cells appear early after hatching in the pronephric kidney, at the level of the ducts of Cuvier and of the cephalic part of the posterior cardinal veins. Later, chromaffin cells in Perciformes reach the anterior cardinal veins, and subsequently, in both Perciformes and Salmoniformes, they reach the developing posterior kidney. Their localization along the posterior kidney is still in progress about 4 months after hatching and is completed about a year after hatching. These findings support the concept that the structure of the adrenal gland in teleosts is intermediate between that of the

  9. Viral Encephalopathy and Retinopathy in groupers (Epinephelus spp. in southern Italy: a threat for wild endangered species?

    Directory of Open Access Journals (Sweden)

    Vendramin Niccolò

    2013-01-01

    Full Text Available Abstract Background Betanodaviruses are the causative agents of Viral Encephalopathy and Retinopathy (VER. To date, more than 50 species have proved to be susceptible and among them, those found in genus Epinephelus are highly represented. Clinical disease outbreaks are generally characterized by typical nervous signs and significant mortalities mainly associated with aquaculture activities, although some concerns for the impact of this infection in wild fish have been raised. In this study, the authors present the first documented report describing an outbreak of VER in wild species in the Mediterranean basin. Case presentation In late summer - early winter 2011 (September-December, significant mortalities affecting wild Dusky grouper (Epinephelus marginatus, Golden grouper (Epinephelus costae and European sea bass (Dicentrarchus labrax were reported in the municipality of Santa Maria di Leuca (Northern Ionian Sea, Italy. The affected fish showed an abnormal swimming behavior and swollen abdomens. During this epizootic, five moribund fish showing clear neurological signs were captured and underwent laboratory investigations. Analytical results confirmed the diagnosis of VER in all the specimens. Genetic characterization classified all betanodavirus isolates as belonging to the RGNNV genotype, revealing a close genetic relationship with viral sequences obtained from diseased farmed fish reared in the same area in previous years. Conclusion The close relationship of the viral sequences between the isolates collected in wild affected fish and those isolated during clinical disease outbreaks in farmed fish in the same area in previous years suggests a persistent circulation of betanodaviruses and transmission between wild and farmed stocks. Further investigations are necessary to assess the risk of viral transmission between wild and farmed fish populations, particularly in marine protected areas where endangered species are present.

  10. PHYTOPLANKTON COMPOSITION IN FISH FARMS ALONG THE EASTERN ADRIATIC COAST

    Directory of Open Access Journals (Sweden)

    Marija Tomec

    2006-10-01

    Full Text Available Investigations of net phytoplankton composition were performed at three fish farms situated at the northern, middle and southern part of the eastern Adriatic Sea coast, respectively. In the northern part investigations were conducted in the Limski kanal, in the middle part at the Ugljan island and in the southern part in the place Drače on the Pelješac peninsula (Figure 1. At all three localities fish culture included mostly two species: gilthead sea bream (Sparus aurata and sea bass (Dicentrarchus labrax. Beside some physico–chemical parameters (sea water temperature, salinity special attention was placed on the examination of qualitative net phytoplankton composition, which was conducted in the period of May and November 2004 and May and October 2005. Samples were collected at the depths of 0. 5 and 4 meters. According to the physico–chemical parameters, sea water temperature was influenced by the temperature of the environment. Qualitative net phytoplankton composition consisted of 153 microphytic species belonging to the systematic compartments of Cyanobacteria, Chrysophyta and Dinophyta (Table 1. The most numerous algal group were diatoms or Bacillarophyceae (84 species or 55% with relative frequencies of species from 1 to 7. Taxonomic composition of diatoms showed the community Chaetoceros–Rhizosolenia (Proboscia as the dominant one. The second numerically most dominant compartment were Dinophyta (62 species or 401% with dominant the species of the genera Ceratium and Protoperidinium. Relative frequencies of species was ranging from 1 to 7 (mass presence of specimens in the water column. From Cyanobacteria (4 species or 3%, only filamentous algae were determined, with individual presence in net phytoplankton composition. Qualitative net phytoplankton composition suggests the similarity of species composition in the water column at all investigated fish farms. From the obtained characteristics of net phytoplankton composition

  11. Assessing chronic fish health: An application to a case of an acute exposure to chemically treated crude oil.

    Science.gov (United States)

    Mauduit, F; Domenici, P; Farrell, A P; Lacroix, C; Le Floch, S; Lemaire, P; Nicolas-Kopec, A; Whittington, M; Zambonino-Infante, J L; Claireaux, G

    2016-09-01

    Human alteration of marine ecosystems is substantial and growing. Yet, no adequate methodology exists that provides reliable predictions of how environmental degradation will affect these ecosystems at a relevant level of biological organization. The primary objective of this study was to develop a methodology to evaluate a fish's capacity to face a well-established environmental challenge, an exposure to chemically dispersed oil, and characterize the long-term consequences. Therefore, we applied high-throughput, non-lethal challenge tests to assess hypoxia tolerance, temperature susceptibility and maximal swimming speed as proxies for a fish's functional integrity. These whole animal challenge tests were implemented before (1 month) and after (1 month) juvenile European sea bass (Dicentrarchus labrax) had been acutely exposed (48h) to a mixture containing 0.08gL(-1) of weathered Arabian light crude oil plus 4% dispersant (Corexit© EC9500A), a realistic exposure concentration during an oil spill. In addition, experimental populations were then transferred into semi-natural tidal mesocosm ponds and correlates of Darwinian fitness (growth and survival) were monitored over a period of 4 months. Our results revealed that fish acutely exposed to chemically dispersed oil remained impaired in terms of their hypoxia tolerance and swimming performance, but not in temperature susceptibility for 1 month post-exposure. Nevertheless, these functional impairments had no subsequent ecological consequences under mildly selective environmental conditions since growth and survival were not impacted during the mesocosm pond study. Furthermore, the earlier effects on fish performance were presumably temporary because re-testing the fish 10 months post-exposure revealed no significant residual effects on hypoxia tolerance, temperature susceptibility and maximal swimming speed. We propose that the functional proxies and correlates of Darwinian fitness used here provide a useful

  12. Quality enhancement in the Japanese sea bass (Lateolabrax japonicas) fillets stored at 4°C by chitosan coating incorporated with citric acid or licorice extract.

    Science.gov (United States)

    Qiu, Xujian; Chen, Shengjun; Liu, Guangming; Yang, Qiuming

    2014-11-01

    The preserving effects of chitosan, chitosan and citric acid, chitosan and licorice extract on fresh Japanese sea bass fillets stored at 4 °C for 12 days were studied. Results showed that citric acid or licorice extract can enhance the preserving function of chitosan significantly by retarding lipid oxidation and inhibiting microbial growth as reflected in thiobarbituric acid reactive substances and total plate count, respectively. Both total volatile basic nitrogen values and sensory scores indicated chitosan and citric acid or licorice extract can significantly reduce the quality loss and extend the shelf life of Japanese sea bass fish fillets during refrigerated storage. Citric acid or licorice extract with chitosan could thus be applied in the seafood industry to enhance quality of fish fillets as natural preservatives.

  13. Active low frequency sound field control in a listening room using CABS (Controlled Acoustic Bass System) will also reduce the sound transmitted to neighbour rooms

    DEFF Research Database (Denmark)

    Nielsen, Sofus Birkedal; Celestinos, Adrian

    2012-01-01

    is possible at modal frequencies. For that reason the modal frequencies in the source room will also have big impact on the transmission to neighbour rooms. These low frequency resonance frequencies are very audible in the source room but also in neighbour rooms as a booming bass. CABS (Controlled Acoustic...... Bass System) is a time based room correction system for reproduced sound using loudspeakers. The system can remove room modes at low frequencies, by active cancelling the reflection from at the rear wall to a normal stereo setup. Measurements in a source room using CABS and in two neighbour rooms have...... shown a reduction in sound transmission of up to 10 dB at resonance frequencies and a reduction at broadband noise of 3 – 5 dB at frequencies up to 100 Hz. The ideas and understanding of the CABS system will also be given....

  14. Anchoring novel molecular biomarker responses to traditional responses in fish exposed to environmental contamination

    Energy Technology Data Exchange (ETDEWEB)

    Nogueira, Patricia [CESAM and Departamento de Biologia, Universidade de Aveiro, Campus de Santiago, 3810-193 Aveiro (Portugal); Department of Biology and Environmental Science, University of Sussex, Falmer, Brighton BN1 9QJ (United Kingdom); Pacheco, Mario [CESAM and Departamento de Biologia, Universidade de Aveiro, Campus de Santiago, 3810-193 Aveiro (Portugal); Lourdes Pereira, M. [CICECO and Departamento de Biologia, Universidade de Aveiro, Campus de Santiago, 3810-193 Aveiro (Portugal); Mendo, Sonia [CESAM and Departamento de Biologia, Universidade de Aveiro, Campus de Santiago, 3810-193 Aveiro (Portugal); Rotchell, Jeanette M., E-mail: J.Rotchell@sussex.ac.u [Department of Biology and Environmental Science, University of Sussex, Falmer, Brighton BN1 9QJ (United Kingdom)

    2010-05-15

    The responses of Dicentrarchus labrax and Liza aurata to aquatic pollution were assessed in a contaminated coastal lagoon, using both traditional and novel biomarkers combined. DNA damage, assessed by comet assay, was higher in both fish species from the contaminated sites, whereas levels of cytochrome P450 1A1 gene expression were not significantly altered. The liver histopathological analysis also revealed significant lesions in fish from contaminated sites. Alterations in ras and xpf genes were analysed and additional pollutant-responsive genes were identified. While no alterations were found in ras gene, a downregulation of xpf gene was observed in D. labrax from a contaminated site. Suppression subtractive hybridization applied to D. labrax collected at a contaminated site, revealed altered expression in genes involved in energy metabolism, immune system activity and antioxidant response. The approach and results reported herein demonstrate the utility of anchoring traditional biomarker responses alongside novel biomarker responses. - Novel molecular biomarkers of aquatic environmental contamination in fish.

  15. Improving oral hygiene skills by computer-based training : A randomized controlled comparison of the modified bass and the fones techniques

    OpenAIRE

    Harnacke, Daniela; Mitter, Simona; Lehner, Marc; Munzert, Jörn; Deinzer, Renate

    2012-01-01

    Background: Gingivitis and other plaque-associated diseases have a high prevalence in western communities even though the majority of adults report daily oral hygiene. This indicates a lack of oral hygiene skills. Currently, there is no clear evidence as to which brushing technique would bring about the best oral hygiene skills. While the modified Bass technique is often recommended by dentists and in textbooks, the Fones technique is often recommended in patient brochures. Still, standardize...

  16. The potential use of legume-based diets supplemented with microbial phytase on the growth performance and feed efficiency of sea bass, Lates calcarifer

    Directory of Open Access Journals (Sweden)

    Erlinda S. Ganzon-Naret

    2013-09-01

    Full Text Available A feeding trial was conducted for 12 weeks to evaluate the potential use of legume-based diets supplemented with dietary microbial phytase on the growth performance and feed efficiency of juvenile sea bass, Lates calcarifer. Fifteen sea bass juveniles (mean initial weight of 0.96 g and mean initial total length (TL of 4.2 cm were stocked at three replicates into each of the twelve 100 L conical fibreglass tanks containing 90 L sea water in a closed recirculating system with filtered and aerated sea water. Four isonitrogenous, isolipidic and isocaloric experimental diets were formulated. The control diet (C0 contained fish meal, soybean meal, shrimp meal and squid meal as major protein sources. Legume seed meals of pigeon pea (Cajanus cajan, yellow mungbeans (Phaseolus aureus, and green mungbeans (Vigna radiata were incorporated in the practical diets D1, D2 and D3 respectively at 18-20% replacing an equivalent amount of 6-7g fish meal protein and supplemented with microbial phytase at the level of 300U kg-1 diet. Growth rate, feed conversion ratio (FCR, protein efficiency ratio (PER and apparent net protein utilization (ANPU of sea bass were significantly (P < 0.05 higher in control diet than those given different legume based diets supplemented with phytase. Histological examination of the liver tissues for the different dietary treatments did not manifest any abnormalities. Phytase supplementation also improved bone ash, phosphorus (P concentration as well as P content in the carcass for fish in legume fed groups. Results from the present study showed that incorporation of dietary microbial phytase in legume based diets slightly improve the growth performance and P availability in sea bass juveniles.

  17. The potential use of legume-based diets supplemented with microbial phytase on the growth performance and feed efficiency of sea bass, Lates calcarifer

    Directory of Open Access Journals (Sweden)

    Erlinda S. Ganzon-Naret

    2013-09-01

    Full Text Available A feeding trial was conducted for 12 weeks to evaluate the potential use of legume-based diets supplemented with dietary microbial phytase on the growth performance and feed efficiency of juvenile sea bass, Lates calcarifer. Fifteen sea bass juveniles (mean initial weight of 0.96 g and mean initial total length (TL of 4.2 cm were stocked at three replicates into each of the twelve 100 L conical fibreglass tanks containing 90 L sea water in a closed recirculating system with filtered and aerated sea water. Four isonitrogenous, isolipidic and isocaloric experimental diets were formulated. The control diet (C0 contained fish meal, soybean meal, shrimp meal and squid meal as major protein sources. Legume seed meals of pigeon pea (Cajanus cajan, yellow mungbeans ((Phaseolus aureus,and green mungbeans ((Vigna radiata were incorporated in the practical diets D1, D2 and D3 respectively at 18-20% replacing an equivalent amount of 6-7g fish meal protein and supplemented with microbial phytase at the level of 300U kg-1 diet. Growth rate, feed conversion ratio (FCR, protein efficiency ratio (PER and apparent net protein utilization (ANPU of sea bass were significantly (P < 0.05 higher in control diet than those given different legume based diets supplemented with phytase. Histological examination of the liver tissues for the different dietary treatments did not manifest any abnormalities. Phytase supplementation also improved bone ash, phosphorus (P concentration as well as P content in the carcass for fish in legume fed groups. Results from the present study showed that incorporation of dietary microbial phytase in legume based diets slightly improve the growth performance and P availability in sea bass juveniles.

  18. Organochlorine pesticides and thiamine in eggs of largemouth bass and American alligators and their relationship with early life-stage mortality

    Science.gov (United States)

    Sepulveda, M.S.; Wiebe, J.J.; Honeyfield, D.C.; Rauschenberger, H.R.; Hinterkopf, J.P.; Johnson, W.E.; Gross, T.S.

    2004-01-01

    Thiamine deficiency has been linked to early mortality syndrome in salmonids in the Great Lakes. This study was conducted to compare thiamine concentrations in American alligators (Alligator mississippiensis) and Florida largemouth bass (Micropterus salmoides floridanus) eggs from sites with high embryo mortality and high exposure to organochlorine pesticides (OCPs) (Lakes Apopka and Griffin, and Emeralda Marsh, Florida, USA) to those from sites that have historically exhibited low embryo mortality and low OCPs (Lakes Woodruff and Orange, Florida). During June-July 2000, 20 alligator clutches were collected from these sites, artificially incubated, and monitored for embryo mortality. Thiamine and OCPs were measured in one egg/clutch. During February 2002, 10 adult female bass were collected from Emeralda Marsh and Lake Woodruff and mature ovaries analyzed for thiamine and OCP concentrations. Although ovaries from the Emeralda Marsh bass contained almost 1,000-fold more OCPs compared with the reference site, Lake Woodruff, there were no differences in thiamine concentrations between sites (11,710 vs. 11,857 pmol/g). In contrast, alligator eggs from the reference site had five times the amount of thiamine compared with the contaminated sites (3,123 vs. 617 pmol/g). Similarly, clutches with > 55% hatch rates had significantly higher concentrations of thiamine compared with clutches with alligator embryo survival but not in reproductive failure and recruitment of largemouth bass. The cause(s) of this thiamine deficiency are unknown but might be related to differences in the nutritional value of prey items across the sites studied and/or to the presence of high concentration of contaminants in eggs. ?? Wildlife Disease Association 2004.

  19. Rates of consumption of juvenile salmonids and alternative prey fish by northern squawfish, walleyes, smallmouth bass, and channel catfish in John Day Reservoir, Columbia River

    International Nuclear Information System (INIS)

    Adult northern squawfish Ptychocheilus oregonesis, walleyes Stizostedion vitreum, smallmouth bass Micropterus dolomieu, and channel catfish Ictalurus punctatus were sampled from four regions of John Day Reservoir from April to August 1983-1986 to quantify their consumption of 13 species of prey fish, particularly seaward-migrating juvenile Pacific salmon and steelhead (Oncorhynchus spp.). Consumption rates were estimated from field data on stomach contents and digestion rate relations determined in previous investigations. For each predator, consumption rates varied by reservoir area, month, time of day, and predator size or age. The greatest daily consumption of salmonids by northern squawfish and channel catfish occurred in the upper end of the reservoir below McNary Dam. Greatest daily predation by walleyes and smallmouth bass occurred in the middle and lower reservoir. Consumption rates of all predators were highest in July, concurrent with maximum temperature and abundance of juvenile salmonids. Feeding by the predators tended to peak after dawn and near midnight. Northern squawfish below McNary Dam exhibited this pattern, but fed mainly in the morning hours down-reservoir. The daily ration of total prey fish was highest for northern squawfish over 451 mm fork length, for walleyes 201-250 mm, for smallmouth bass 176-200 mm, and for channel catfish 401-450 mm. Averaged over all predator sizes and sampling months (April-August), the total daily ration (fish plus other prey) of smallmouth bass was about twice that of channel catfish, northern squawfish, and walleyes. However, northern squawfish was clearly the major predator on juvenile salmonids

  20. Anoplastie périnéale simple pour le traitement des malformations anorectales basses chez l'adulte, à propos de deux cas

    Science.gov (United States)

    Echchaoui, Abdelmoughit; Benyachou, Malika; Hafidi, Jawad; Fathi, Nahed; Mohammadine, Elhamid; ELmazouz, Samir; Gharib, Nour-eddine; Abbassi, Abdellah

    2014-01-01

    Les malformations anorectales chez l'adulte sont des anomalies congénitales rares du tube digestif qui prédominent chez le sexe féminin. Notre étude porte sur deux observations de malformation anorectale basses vues et traitées au stade adulte par les 2 équipes (plasticiens et viscéralistes) à l'Hôpital Avicenne à Rabat. Il s'agit d'un homme de 24 ans avec une dyschésie anale l'autre cas est une femme de 18 ans avec une malformation anovulvaire Les caractéristiques cliniques combinées avec les imageries radiologiques (lavement baryté, et la manométrie anorectale) ont confirmé qu'il s'agit d'une malfomation anorectale basse. Les deux cas sont corrigés par une reconstruction sphinctérienne, réimplantation anale avec anoplastie périnéale. Les suites opératoires étaient simples, pas de souffrance cutanée ou nécrose, avec changement de pansement gras chaque jour. Le résultat fonctionnel (la continence) était favorable pour les 2 patients. La présentation des MAR à l’âge adulte est rare, d’étiologie mal connu, elles apparaissent selon le mode sporadique. Les caractéristiques cliniques, couplées à l'imagerie (lavement baryté, IRM pelvienne), l'endoscopie et la manométrie anorectale, permettent de confirmer le diagnostic et classer ces anomalies en 3 types: basses, intermédiaires, et hautes. Les formes basses sont traités d'emblée par une réimplantation anale et anoplastie périnéale simple tels nos deux cas, elles peuvent être traités dans certains cas par un abaissement anorectale associé à une plastie V-Y permettant ainsi un emplacement anatomique correct de l'anus; alors que les formes hautes ou intermédiaires relèvent d'une chirurgie complexe avec souvent une dérivation digestive transitoire. Contrairement aux autres formes, Les formes basses ont un pronostic fonctionnel favorable. PMID:25667689

  1. Effects of the pesticide methoxychlor on gene expression in the liver and testes of the male largemouth bass (Micropterus salmoides).

    Science.gov (United States)

    Blum, Jason L; Nyagode, Beatrice A; James, Margaret O; Denslow, Nancy D

    2008-03-26

    The organochlorine pesticide methoxychlor (MXC) is an environmental estrogen known to stimulate the expression of the egg-yolk protein, vitellogenin (Vtg) in fish species. To begin to understand the underlying mechanisms for how MXC exerts its deleterious effects on the endocrine system, male largemouth bass (Micropterus salmoides) were treated with 2.5, 10, or 25mg/kg MXC and compared to fish pair-treated with 1mg/kg 17 beta-estradiol (E2), and vehicle control. Fish were sacrificed 24, 48, or 72 h following treatment. The liver and testes were then assayed for changes in expression of the three bass estrogen receptors (ERs alpha, beta a, and beta b) in tissues, as well as Vtg and cytochrome P450 (CYP) 3A isoform 68 in the liver and steroidogenic acute regulatory protein (StAR) in the testes. In the liver, significant increases in gene expression were seen for each of the genes measured by 24 h and each returned to the level of the vehicle by 72 h. Total testosterone 6 beta-hydroxylase activity, reflective of CYP3A activity, was also increased by 24h for all of the exposures. In the testes, ER alpha was unaffected by any treatment, ERbetaa was up-regulated only by MXC, peaking at 24h for the 2.5 and 10mg/kg MXC and at 48 h for the 25mg/kg MXC treatment. By 72 h, the MXC effects had disappeared, while E2 significantly decreased the expression of ER beta a mRNA. ER beta b expression in the testes was stimulated by all concentrations of MXC by 24 h and the effect remained up to 72 h, whereas E2 had no effect. Finally, StAR expression was also found to be decreased by E2 and all MXC treatments. However, the effect on StAR expression by E2 occurred within 24h, while the effect by all concentrations of MXC was not seen until 72 h after treatment. The stimulatory effects of E2 and 25mg/kg MXC on the expression of the ERs in the liver were opposite to the responses seen in the testes, suggesting an inverted relationship between these two tissue types. These results provide

  2. Disparate effects of constant and annually-cycling daylength and water temperature on reproductive maturation of striped bass (Morone saxatilis)

    Science.gov (United States)

    Clark, R.W.; Henderson-Arzapalo, A.; Sullivan, C.V.

    2005-01-01

    Adult striped bass (Morone saxatilis) were exposed to various combinations of constant or anually-cycling daylength and water temperature. Constant conditions (15 h days, 18??C) were those normally experienced at spawning and cycling conditions simulated natural changes at Chesapeake Bay latitude. Females exposed to constant long (15 h) days and cycling water temperature (TEMPERATURE group) had blood plasma levels of sex steroids (testosterone [T] and estradiol-17?? [E2]) and vitellogenin (Vg), and profiles of oocyte growth, that were nearly identical to those of females held under a natural photothermal cycle (CONTROL group). Several fish from these two groups were induced to spawn fertile eggs. Females constantly exposed to warm water (18??C), with or without a natural photoperiod cycle (PHOTOPERIOD and STATIC groups, respectively), had diminished circulating levels of gonadal steroid hormones and Vg, impaired deposition of yolk granules in their ooplasm, and decreased oocyte growth, and they underwent premature ovarian atresia. Males exposed to cycling water temperature (CONTROL and TEMPERATURE groups) spermiated synchronously during the natural breeding season, at which time they also had had high plasma androgen (T and 11-ketotestosterone [11-KT]) levels. The timing of spermiation was highly asynchronous among males in groups of fish held constantly at 18??C (STATIC and PHOTOPERIOD groups) and this asynchrony was associated with diminished plasma androgen levels. Termination of spermiation by males exposed to cycling water temperature coincided with a sharp decline in levels of plasma androgens about a month after water temperature rose above 18??C. In contrast, most males held constantly at 18??C sustained intermediate levels of plasma androgens and spermiated until the end of the study in late July. The annual cycle of water temperature clearly plays a prominent role in the initiation, maintenance, and termination of the striped bass reproductive cycle. In

  3. Mercury in fish scales as an assessment method for predicting muscle tissue mercury concentrations in largemouth bass.

    Science.gov (United States)

    Lake, J L; Ryba, S A; Serbst, J R; Libby, A D

    2006-05-01

    The relationship between total mercury (Hg) concentration in fish scales and in tissues of largemouth bass (Micropterus salmoides) from 20 freshwater sites was developed and evaluated to determine whether scale analysis would allow a nonlethal and convenient method for predicting Hg concentrations in tissues. The relationship between total Hg concentration in untreated scale samples and muscle tissue is highly variable. Several different scale treatments were tried in an effort to increase the coefficient of determination and thereby enhance the effectiveness of this predictive technique. Washing scales with acetone, deionized (DI) water, detergent solution, and soap were used in conjunction with ultrasonication. The use of a mild soap solution with heating and ultrasonication increased the r(2) the most (from 0.69 [untreated scales] to 0.89). However, despite treatment, wide predictions of tissue Hg concentration remained. These results suggest that application of this technique as an independent method for issuance of fish advisories is inappropriate. Nevertheless, our results showed that scale analysis has potential for assessing general trends in concentration relative to a tissue criterion and for assessing Hg contamination in fish tissue as a first-level screen. PMID:16435082

  4. Concentrations of mercury and other metals in black bass (Micropterus spp.) from Whiskeytown Lake, Shasta County, California, 2005

    Science.gov (United States)

    May, Jason T.; Hothem, Roger L.; Bauer, Marissa L.; Brown, Larry R.

    2012-01-01

    This report presents the results of a reconnaissance study conducted by the U.S. Geological Survey (USGS) to determine mercury (Hg) and other selected metal concentrations in Black bass (Micropterus spp.) from Whiskeytown Lake, Shasta County, California. Total mercury concentrations were determined by cold-vapor atomic absorption spectroscopy (CVAAS) in fillets and whole bodies of each sampled fish. Selected metals scans were performed on whole bodies (less the fillets) by inductively coupled plasma–mass spectroscopy (ICP-MS) and inductively coupled plasma–optical emission spectroscopy (ICP-OES). Mercury concentrations in fillet samples ranged from 0.06 to 0.52 micrograms per gram (μg/g) wet weight (ww). Total mercury (HgT) in the same fish whole-body samples ranged from 0.04 to 0.37 (μg/g, ww). Mercury concentrations in 17 percent of "legal catch size" (≥305 millimeters in length) were above the U.S. Environmental Protection Agency water-quality criterion for the protection of human health of 0.30 μg/g (ww). These data will serve as a baseline for future monitoring efforts within Whiskeytown Lake.

  5. The C-Band All-Sky Survey (C-BASS): Design and implementation of the northern receiver

    CERN Document Server

    King, O G; Blackhurst, E J; Copley, C; Davis, R J; Dickinson, C; Holler, C M; Irfan, M O; John, J J; Leahy, J P; Leech, J; Muchovej, S J C; Pearson, T J; Stevenson, M A; Taylor, Angela C

    2013-01-01

    The C-Band All-Sky Survey (C-BASS) is a project to map the full sky in total intensity and linear polarization at 5 GHz. The northern component of the survey uses a broadband single-frequency analogue receiver fitted to a 6.1-m telescope at the Owens Valley Radio Observatory in California, USA. The receiver architecture combines a continuous-comparison radiometer and a correlation polarimeter in a single receiver for stable simultaneous measurement of both total intensity and linear polarization, using custom-designed analogue receiver components. The continuous-comparison radiometer measures the temperature difference between the sky and temperature-stabilized cold electrical reference loads. A cryogenic front-end is used to minimize receiver noise, with a system temperature of $\\approx 30$ K in both linear polarization and total intensity. Custom cryogenic notch filters are used to counteract man-made radio frequency interference. The radiometer $1/f$ noise is dominated by atmospheric fluctuations, while th...

  6. Mycobacterium pseudoshottsii sp. nov., a slowly growing chromogenic species isolated from Chesapeake Bay striped bass (Morone saxatilis)

    Science.gov (United States)

    Rhodes, M.W.; Kator, H.; McNabb, A.; Deshayes, C.; Reyrat, J.-M.; Brown-Elliott, B. A.; Wallace, R.; Trott, K.A.; Parker, J.M.; Lifland, B.; Osterhout, G.; Kaattari, I.; Reece, K.; Vogelbein, W.; Ottinger, C.A.

    2005-01-01

    A group of slowly growing photochromogenic mycobacteria was isolated from Chesapeake Bay striped bass (Morone saxatilis) during an epizootic of mycobacteriosis. Growth characteristics, acid-fastness and 16S rRNA gene sequencing results were consistent with those of the genus Mycobacterium. Biochemical reactions, growth characteristics and mycolic acid profiles (HPLC) resembled those of Mycobacterium shottsii, a non-pigmented mycobacterium also isolated during the same epizootic. Sequencing of the 16S rRNA genes, the gene encoding the exported repeated protein (erp) and the gene encoding the 65 kDa heat-shock protein (hsp65) and restriction enzyme analysis of the hsp65 gene demonstrated that this group of isolates is unique. Insertion sequences associated with Mycobacterium ulcerans, IS2404 and IS2606, were detected by PCR. These isolates could be differentiated from other slowly growing pigmented mycobacteria by their inability to grow at 37 ??C, production of niacin and urease, absence of nitrate reductase, negative Tween 80 hydrolysis and resistance to isoniazid (1 ??g ml-1), p-nitrobenzoic acid, thiacetazone and thiophene-2-carboxylic hydrazide. On the basis of this polyphasic study, it is proposed that these isolates represent a novel species, Mycobacterium pseudoshottsii sp. nov. The type strain, L15T, has been deposited in the American Type Culture Collection as ATCC BAA-883T and the National Collection of Type Cultures (UK) as NCTC 13318T. ?? 2005 IUMS.

  7. Selective analysis of power plant operation on the Hudson River with emphasis on the Bowline Point Generating Station. Volume 1. [Effects on striped bass population

    Energy Technology Data Exchange (ETDEWEB)

    Barnthouse, L. W.; Cannon, J. B.; Christensen, S. G.

    1977-07-01

    A comprehensive study of the effects of power plant operation on the Hudson River was conducted. The study included thermal, biological, and air quality effects of existing and planned electrical generating stations. This section on thermal impacts presents a comprehensive mathematical modeling and computer simulation study of the effects of heat rejection from the plants. The overall study consisted of three major parts: near-field analysis; far-field analysis; and zone-matched near-field/far-field analysis. Near-field analyses were completed for Roseton, Danskammer, and Bowline Point Generating Stations, and near-field dilution ratios range from a low of about 2 for Bowline Point and 3 for Roseton to a maximum of 6 for both plants. The far-field analysis included a critical review of existing studies and a parametric review of operating plants. The maximum thermal load case, based on hypothetical 1974 river conditions, gives the daily maximum cross-section-averaged and 2-mile-segment-averaged water temperatures as 83.80/sup 0/F in the vicinity of the Indian Point Station and 83.25/sup 0/F in the vicinity of the Bowline Station. This maximum case will be significantly modified if cooling towers are used at certain units. A full analysis and discussion of these cases is presented. A study of the Hudson River striped bass population is divided into the following eight subsections: distribution of striped bass eggs, larvae, and juveniles in the Hudson River; entrainment mortality factor; intake factor; impingement; effects of discharges; compensation; model estimates of percent reduction; and Hudson River striped bass stock.

  8. EVALUACIÓN DE LAS TÉCNICAS DE CEPILLADO DE BASS Y VERTICAL EN UN GRUPO DE PACIENTES EN TRATAMIENTO DE ORTODONCIA CON TÉCNICA MBT

    Directory of Open Access Journals (Sweden)

    Yelitza Ivone Baracaldo Ortíz

    2012-12-01

    Full Text Available Objetivo: Evaluar la efectividad de las técnicas de Cepillado de Bass y Vertical en pacientes con aparatología ortodóntica fija tratados con la técnica MBT. Materiales y métodos: se realizó un estudio experimental “In vivo” aleatorizado doble ciego; la muestra fue de 100 personas distribuidas en dos grupos, a cada uno se le asignó una Técnica de Cepillado y se dieron instrucciones de higiene oral por medio de charlas personalizadas y por escrito. Se usó el índice de Sillnes y Löe para medir el porcentaje de placa bacteriana, el cual fue realizado por una higienista oral, cada 30 ± 3 días durante 3 meses. A los dos grupos se les realizó profilaxis con el objetivo de tener un índice inicial de placa lo más aproximado a cero. A los participantes se les entregó mensualmente los aditamentos de higiene oral que debían usar. Resultados: antes de dar instrucciones de higiene oral el 73,4% tenían índice de placa moderado, el 16,46% severo y el 10,3% incipiente. El grupo de Técnica Vertical obtuvo mayor reducción de placa en comparación con el grupo de Técnica  Bass durante la primera toma (pConclusiones: La Técnica de Cepillado Vertical es más efectiva que la Técnica Bass para disminuir la placa bacteriana en pacientes tratados ortodónticamente con la técnica MBT.  

  9. Natural history of SLC11 genes in vertebrates: tales from the fish world

    Directory of Open Access Journals (Sweden)

    Castro L Filipe C

    2011-04-01

    Full Text Available Abstract Background The SLC11A1/Nramp1 and SLC11A2/Nramp2 genes belong to the SLC11/Nramp family of transmembrane divalent metal transporters, with SLC11A1 being associated with resistance to pathogens and SLC11A2 involved in intestinal iron uptake and transferrin-bound iron transport. Both members of the SLC11 gene family have been clearly identified in tetrapods; however SLC11A1 has never been documented in teleost fish and is believed to have been lost in this lineage during early vertebrate evolution. In the present work we characterized the SLC11 genes in teleosts and evaluated if the roles attributed to mammalian SLC11 genes are assured by other fish specific SLC11 gene members. Results Two different SLC11 genes were isolated in the European sea bass (Dicentrarchus. labrax, and named slc11a2-α and slc11a2-β, since both were found to be evolutionary closer to tetrapods SLC11A2, through phylogenetic analysis and comparative genomics. Induction of slc11a2-α and slc11a2-β in sea bass, upon iron modulation or exposure to Photobacterium damselae spp. piscicida, was evaluated in in vivo or in vitro experimental models. Overall, slc11a2-α was found to respond only to iron deficiency in the intestine, whereas slc11a2-β was found to respond to iron overload and bacterial infection in several tissues and also in the leukocytes. Conclusions Our data suggests that despite the absence of slc11a1, its functions have been undertaken by one of the slc11a2 duplicated paralogs in teleost fish in a case of synfunctionalization, being involved in both iron metabolism and response to bacterial infection. This study provides, to our knowledge, the first example of this type of sub-functionalization in iron metabolism genes, illustrating how conserving the various functions of the SLC11 gene family is of crucial evolutionary importance.

  10. MARICULTURE ON CROATIAN ISLANDS

    Directory of Open Access Journals (Sweden)

    Gordana Šarušić

    2000-09-01

    Full Text Available The first attempts of intensive mariculture in Croatia commenced at the very beginning of 1980’s. The mid-eighties brought an expansion of mariculture production, which has been continuously increasing. A few different marine organisms are intensively cultured - both fish and shellfish. Among them commercially most important and highly valued species are sea bass Dicentrarchus labrax and sea bream Sparus aurata. Mussel Mytilus galloprovincialis and oyster Ostrea edulis are the most important shellfish. Fish species such as dentex Dentex dentex, red sea bream Pagrus major and sheepshead bream Puntazzo puntazzo are reared too, but in a rather small quantities. Only recently the rearing, on-growing- of bluefin tuna Thunnus thynnus started in Croatia. The juveniles (70% are reared in a Croatian hatcheries, and 30% has to be imported mainly from Italy and France, due to a higher demand for this kind of culture among the small growers. Croatian part of Adriatic sea possesses a number of geomorfologicaly suitable sites and meteorological conditions which determined the choice - type - of intensive culture. All fish species are reared in a floating cages. The choice of cages i. e. semi off-shore or floating frames, size, rearing volume and design depend on the investors personal preference. The annual turnouf of a market size bass was about 600t and 300t bream in 1996., by 10 island farms which is 70% of total production in Croatia. Including other cultured fish species last year production was up to 1000t, and it™s being estimated to be about 1300t in the following year. The shellfish production on the islands is usually individual attempt of farmers, producing minor quantities mostly in polyculture. This production has bigger potential but it’s limited owing to the EU quality control regulations which do not allow the export, and by domestic market which has drastically decreased due to the collapse of tourism during the recent war. Almost 80

  11. MORPHOLOGICAL AND ELECTROPHYSIOLOGICAL STUDIES OF HORIZONTAL CELLS IN THE BLACK BASS RETINA(Issue Commemorating the Directorship of Prof. Yoko HASHIMOTO, at the Department of Physiology)

    OpenAIRE

    UMINO, Osamu; MAEHARA, Michiyo; HASHIMOTO, Yoko; 海野, 修; 前原, 通代; 橋本, 葉子

    1997-01-01

    The purpose of this study is to examine the correlation between the morphological characteristics and spectral responses of horizontal cells in black bass retina. Three layers of horizontal cell bodies and one layer of fine processes of horizontal cell axons were recognized in the horizontal cell layer. The most distally layered H1 cells were smaller than the H2 cells which form the second layer. Both H1 and H2 cells showed a monophasic spectral response with a maximum peak for red light. In ...

  12. Low frequency sound field control in rectangular listening rooms using CABS (Controlled Acoustic Bass System) will also reduce sound transmission to neighbor rooms

    DEFF Research Database (Denmark)

    Nielsen, Sofus Birkedal; Celestinos, Adrian

    2011-01-01

    Sound reproduction is often taking place in small and medium sized rectangular rooms. As rectangular rooms have 3 pairs of parallel walls the reflections at especially low frequencies will cause up to 30 dB spatial variations of the sound pressure level in the room. This will take place not only at...... resonance frequencies, but more or less at all frequencies. A time based room correction system named CABS (Controlled Acoustic Bass System) has been developed and is able to create a homogeneous sound field in the whole room at low frequencies by proper placement of multiple loudspeakers. A normal setup...

  13. Studies on the nutrition of spotted sand bass, Paralabrax maculatofasciatus. Effect of the dietary protein level on growth and protein utilization in juveniles fed semipurified diets

    OpenAIRE

    Anguas Vélez, Benjamín H.; Civera Cerecedo, Roberto; Cadena Roa, Marco; Guillaume, Juan; Martínez Díaz, Sergio Francisco

    2000-01-01

    Two feeding trials were conducted to determine the digestibility of a casein-based semi­purified diet and the effects of different protein levels on growth and protein use of spotted sand bass Paralabrax maculatofascÜltUs juveniles. For trial 1, a semipurified diet with vitamin­free casein as the sole source of protein was fed three times a day to apparent satiation, for a period of 20 d. Feces were collected by siphoning each tank. The digestibility of the exper­imental diet was ...

  14. Modification of fillet composition and evidence of differential fatty acid turnover in sunshine bass Morone chrysops x M. saxatilis following change in dietary lipid source.

    Science.gov (United States)

    Lane, Ryan L; Trushenski, Jesse T; Kohler, Christopher C

    2006-11-01

    Marine oil-based finishing diets have been used to restore fillet FA profile in several "medium-fat" fleshed aquaculture species, and a simple dilution model describing FA turnover has been established to predict and tailor final fillet composition. We evaluated finishing diet efficacy and suitability of the dilution model to describe patterns of FA change in a lean-fleshed model, sunshine bass. Two practical diets (45% crude protein, 15% crude lipid) were formulated, respectively containing corn oil (CO) or menhaden oil (MO) as the primary lipid sources. Sunshine bass (age 1 [approximately 14 mo], 347 +/- 8.6 g, mean individual weight +/- SEM) were stocked in a recirculating system and fed the diets according to different feeding regimens during the final 28 wk of the production cycle. Control groups were fed the CO or the MO feeds exclusively; whereas, the remaining treatment groups were transitioned from the CO diet to the MO diet at 4-, 8-, or 12-wk intervals. Upon completion of the feeding trial, fish were harvested, and production performance and fillet composition were assessed. Replacing MO with CO as the primary lipid source in sunshine bass diets yielded fillets with distinctly different FA profiles; however, finishing with a MO-based diet offered significant compensation for CO-associated reductions in fillet long-chain highly unsaturated FA (LC-HUFA). Although complete restoration was not observed, we achieved significant augmentation of endogenous n-3 FA within 4 wk of feeding the MO diet, and observed a significant increase in LC-HUFA and a beneficial shift in n-3:n-6 FA ratio after 8 weeks. Simple dilution accurately predicted tissue composition for most FA; however, deviations from the model were noted, suggesting selective retention of n-3, PUFA, and LC-HUFA and preferential catabolism of saturates. We conclude marine oil-based finishing diets can rapidly augment beneficial FA levels in sunshine bass fillets; however, simple dilution models do not

  15. Report of the Inter-Benchmark Protocol on New Species (Turbot and Sea bass ; IBPNew 2012). 1-5 October 2012 Copenhagen, Denmark

    OpenAIRE

    ICES

    2012-01-01

    Inter-Benchmark Workshop on NEW species (IBPNEW) met in Copenhagen (Den-mark) from 1–5 October 2012 to perform a benchmark assessment on the stocks of turbot and sea bass. All the terms of reference were addressed. In hindsight, the five days that were available for this workshop were too short. Although most work re-garding the ToRs was finished before the end of the workshop, large parts of the report still needed to be finished at the end of the meeting, and this caused consider-able delay...

  16. High contaminant loads in Lake Apopka's riparian wetland disrupt gene networks involved in reproduction and immune function in largemouth bass.

    Science.gov (United States)

    Martyniuk, Christopher J; Doperalski, Nicholas J; Prucha, Melinda S; Zhang, Ji-Liang; Kroll, Kevin J; Conrow, Roxanne; Barber, David S; Denslow, Nancy D

    2016-09-01

    Lake Apopka (FL, USA) has elevated levels of some organochlorine pesticides in its sediments and a portion of its watershed has been designated a US Environmental Protection Agency Superfund site. This study assessed reproductive endpoints in Florida largemouth bass (LMB) (Micropterus salmoides floridanus) after placement into experimental ponds adjacent to Lake Apopka. LMB collected from a clean reference site (DeLeon Springs) were stocked at two periods of time into ponds constructed in former farm fields on the north shore of the lake. LMB were stocked during early and late oogenesis to determine if there were different effects of contamination on LMB that may be attributed to their reproductive stage. LMB inhabiting the ponds for ~4months had anywhere from 2 to 800 times higher contaminant load for a number of organochlorine pesticides (e.g. p, p'-DDE, methoxychlor) compared to control animals. Gonadosomatic index and plasma vitellogenin were not different between reproductively-stage matched LMB collected at reference sites compared to those inhabiting the ponds. However, plasma 17β-estradiol was lower in LMB inhabiting the Apopka ponds compared to ovary stage-matched LMB from the St. Johns River, a site used as a reference site. Sub-network enrichment analysis revealed that genes related to reproduction (granulosa function, oocyte development), endocrine function (steroid metabolism, hormone biosynthesis), and immune function (T cell suppression, leukocyte accumulation) were differentially expressed in the ovaries of LMB placed into the ponds. These data suggest that (1) LMB inhabiting the Apopka ponds showed disrupted reproduction and immune responses and that (2) gene expression profiles provided site-specific information by discriminating LMB from different macro-habitats. PMID:27397556

  17. Ovary transcriptome profiling via artificial intelligence reveals a transcriptomic fingerprint predicting egg quality in striped bass, Morone saxatilis.

    Directory of Open Access Journals (Sweden)

    Robert W Chapman

    Full Text Available Inherited gene transcripts deposited in oocytes direct early embryonic development in all vertebrates, but transcript profiles indicative of embryo developmental competence have not previously been identified. We employed artificial intelligence to model profiles of maternal ovary gene expression and their relationship to egg quality, evaluated as production of viable mid-blastula stage embryos, in the striped bass (Morone saxatilis, a farmed species with serious egg quality problems. In models developed using artificial neural networks (ANNs and supervised machine learning, collective changes in the expression of a limited suite of genes (233 representing 90% of the eventual variance in embryo survival. Egg quality related to minor changes in gene expression (<0.2-fold, with most individual transcripts making a small contribution (<1% to the overall prediction of egg quality. These findings indicate that the predictive power of the transcriptome as regards egg quality resides not in levels of individual genes, but rather in the collective, coordinated expression of a suite of transcripts constituting a transcriptomic "fingerprint". Correlation analyses of the corresponding candidate genes indicated that dysfunction of the ubiquitin-26S proteasome, COP9 signalosome, and subsequent control of the cell cycle engenders embryonic developmental incompetence. The affected gene networks are centrally involved in regulation of early development in all vertebrates, including humans. By assessing collective levels of the relevant ovarian transcripts via ANNs we were able, for the first time in any vertebrate, to accurately predict the subsequent embryo developmental potential of eggs from individual females. Our results show that the transcriptomic fingerprint evidencing developmental dysfunction is highly predictive of, and therefore likely to regulate, egg quality, a biologically complex trait crucial to reproductive fitness.

  18. Age, growth, mortality, and reproduction of Roughtongue bass, Pronotogrammus martinicensis 9Serranidae), in the northeastern Gulf of Mexico

    Science.gov (United States)

    McBride, Richard S.; Sulak, Kenneth J.; Thurman, Paul E.; Richardson, Adam K.

    2009-01-01

    The inaccessibility of outer continental shelf reefs has made it difficult to investigate the biology of Pronotogrammus martinicensis, a small sea bass known to be numerous and widely distributed in such habitat. This study takes advantage of a series of cruises in the northeastern Gulf of Mexico that collected 1,485 individuals. Fish were collected over or in the vicinity of reef habitats with hook and line, otter trawl, and rotenone. We present a preliminary validation of an otolith ageing method and report that P. martinicensis reached a maximum size of 143 mm standard length (SL), grew to about 50% of this size within their first year, and lived to a maximum age of 15 yr. Size at age data (n = 490) fitted to the von Bertalanffy growth model yielded the predictive equation: SLt = 106.3(1 2 e [20.641{t20.646}]), where t = age in years. Gonad histology (n = 333) was examined to confirm that P. martinicensis is a protogynous, monandric hermaphrodite. We found no evidence of simultaneous hermaphroditism, which had been tentatively proposed in a previous study. Most P. martinicensis matured as females in their second year (age 1), primary oocytes developed asynchronously into secondary oocytes, and females were batch spawners. Males were postmaturational. Seminiferous tissue formed as early as age 1, but, although the rate of sex change is unknown, most fish did not function as a male until age 3 or age 4. These data provide age-based benchmarks of a common reef fish species living on the outer continental shelf of the tropical western North Atlantic Ocean.

  19. Ovary Transcriptome Profiling via Artificial Intelligence Reveals a Transcriptomic Fingerprint Predicting Egg Quality in Striped Bass, Morone saxatilis

    Science.gov (United States)

    2014-01-01

    Inherited gene transcripts deposited in oocytes direct early embryonic development in all vertebrates, but transcript profiles indicative of embryo developmental competence have not previously been identified. We employed artificial intelligence to model profiles of maternal ovary gene expression and their relationship to egg quality, evaluated as production of viable mid-blastula stage embryos, in the striped bass (Morone saxatilis), a farmed species with serious egg quality problems. In models developed using artificial neural networks (ANNs) and supervised machine learning, collective changes in the expression of a limited suite of genes (233) representing 90% of the eventual variance in embryo survival. Egg quality related to minor changes in gene expression (<0.2-fold), with most individual transcripts making a small contribution (<1%) to the overall prediction of egg quality. These findings indicate that the predictive power of the transcriptome as regards egg quality resides not in levels of individual genes, but rather in the collective, coordinated expression of a suite of transcripts constituting a transcriptomic “fingerprint”. Correlation analyses of the corresponding candidate genes indicated that dysfunction of the ubiquitin-26S proteasome, COP9 signalosome, and subsequent control of the cell cycle engenders embryonic developmental incompetence. The affected gene networks are centrally involved in regulation of early development in all vertebrates, including humans. By assessing collective levels of the relevant ovarian transcripts via ANNs we were able, for the first time in any vertebrate, to accurately predict the subsequent embryo developmental potential of eggs from individual females. Our results show that the transcriptomic fingerprint evidencing developmental dysfunction is highly predictive of, and therefore likely to regulate, egg quality, a biologically complex trait crucial to reproductive fitness. PMID:24820964

  20. Are endocrine and reproductive biomarkers altered in contaminant-exposed wild male Largemouth Bass (Micropterus salmoides) of Lake Mead, Nevada/Arizona, USA?

    Science.gov (United States)

    Goodbred, Steven L; Patiño, Reynaldo; Torres, Leticia; Echols, Kathy R; Jenkins, Jill A; Rosen, Michael R; Orsak, Erik

    2015-08-01

    Male Largemouth Bass were sampled from two locations in Lake Mead (USA), a site influenced by treated municipal wastewater effluent and urban runoff (Las Vegas Bay), and a reference site (Overton Arm). Samples were collected in summer (July '07) and spring (March '08) to assess general health, endocrine and reproductive biomarkers, and compare contaminant body burdens by analyzing 252 organic chemicals. Sperm count and motility were measured in spring. Contaminants were detected at much higher frequencies and concentrations in fish from Las Vegas Bay than Overton Arm. Those with the highest concentrations included PCBs, DDTs, PBDEs, galaxolide, and methyl triclosan. Fish from Las Vegas Bay also had higher Fulton condition factor, hepatosomatic index, and hematocrit, and lower plasma 11-ketotestosterone concentration (KT). Gonadosomatic index (GSI) and sperm motility did not differ between sites, but sperm count was lower by nearly 50% in fish from Las Vegas Bay. A positive association between KT and GSI was identified, but this association was nonlinear. On average, maximal GSI was reached at sub-maximal KT concentrations. In conclusion, the higher concentration of contaminant body burdens coupled with reduced levels of KT and sperm count in fish from Las Vegas Bay suggest that male reproductive condition was influenced by contaminant exposures. Also, the nonlinear KT-GSI association provided a framework to understand why GSI was similar between male bass from both sites despite their large difference in KT, and also suggested the existence of post-gonadal growth functions of KT at high concentrations. PMID:25733205

  1. High prevalence of buccal ulcerations in largemouth bass, Micropterus salmoides (Centrarchidae from Michigan inland lakes associated with Myzobdella lugubris Leidy 1851 (Annelida: Hirudinea

    Directory of Open Access Journals (Sweden)

    Faisal M.

    2011-02-01

    Full Text Available Widespread mouth ulcerations were observed in largemouth bass collected from eight inland lakes in the Lower Peninsula of Michigan during the summer months of 2002 and 2003. These ulcerations were associated with, and most likely caused by, leech parasitism. Through the use of morphological dichotomous keys, it was determined that all leeches collected are of one species: Myzobdella lugubris. Among the eight lakes examined, Lake Orion and Devils Lake had the highest prevalence of leech parasitism (34% and 29%, respectively and mouth ulcerations (53% and 68%, respectively. Statistical analyses demonstrated that leech and ulcer prevalence varied significantly from one lake to the other. Additionally, it was determined that the relationship between the prevalence of ulcers and the prevalence of leech attachment is significant, indicating that leech parasitism is most likely the cause of ulceration. The ulcers exhibited deep hemorrhagic centers and raised irregular edges. Affected areas lost their epithelial lining and submucosa, with masses of bacteria colonizing the damaged tissues. Since largemouth bass is a popular global sportfish and critical to the food web of inland lakes, there are concerns that the presence of leeches, damaged buccal mucosa, and general unsightliness may negatively affect this important sportfishery.

  2. A comparison of mercury burdens between St. Marks National Wildlife Refuge and St. Andrew Bay, Florida: Evaluation of fish body burdens and physiological responses in largemouth bass, spotted seatrout, striped mullet, and sunfish

    Science.gov (United States)

    Huge, D.H.; Rauschenberger, R.H.; Wieser, F.M.; Hemming, J.M.

    2011-01-01

    Musculature from the dorsal region of 130 largemouth bass (Micropterus salmoides), 140 sunfish (Lepomis sp.), 41 spotted seatrout (Cynoscion nebulosus) and 67 striped mullet (Mugil cephalus) were collected from five estuarine and five freshwater sites within the St. Marks National Wildlife Refuge and two estuarine and two freshwater sites from St. Andrew Bay, Florida, United States of America. Musculature was analyzed for total mercury content, sagittal otoliths were removed for age determination and physiological responses were measured. Largemouth bass and sunfish from the refuge had higher mercury concentrations in musculature than those from the bay. Male spotted seatrout, male striped mullet, male and female sunfish and female largemouth bass had mercury burdens positively correlated with length. The majority of all four species of fish from both study areas contained mercury levels below 1.5 part per million, the limit for safe consumption recommended the Florida Department of Health. In comparison, a significant percentage of largemouth bass and sunfish from several sampled sites, most notably Otter Lake and Lake Renfroe within St. Marks National Wildlife Refuge, had mercury levels consistent with the health department's guidelines of 'limited consumption' or 'no consumption guidelines.'

  3. Apparent digestibility of Asian carp and common carp-derived fish meals in feeds for hybrid striped bass Morone saxatilis female x M. chrysops male and rainbow trout Oncorhynchus mykiss

    Science.gov (United States)

    Apparent digestibility coefficients (ADCs) of nutrients (crude protein, amino acids, crude lipid, fatty acids, and minerals) were determined for fish meals derived from menhaden, Asian carp (combination of silver and bighead carps), and common carp in feeds for hybrid striped bass and rainbow trout....

  4. Efficacy of florfenicol, copper sulfate and potassium permanganate in controlling a natural infection of Aeromonas hydrophila and Flavobacterium columnare in sunshine bass, Morone chrysops female x Morone saxatilis male

    Science.gov (United States)

    Sunshine bass (Morone chrysops female ' Morone saxatilis male) naturally infected with Aeromonas hydrophila and Flavobacterium columnare were randomly assigned to six treatments: 1) two treatments of waterborne exposures to copper sulfate (CuSO4), at 2.1 and at 4.2 mg/L (approximately one and two pe...

  5. Partial pressure (or fugacity) of carbon dioxide, salinity and other variables collected from underway - surface observations using Carbon dioxide (CO2) gas analyzer, Shower head chamber equilibrator for autonomous carbon dioxide (CO2) measurement and other instruments from the SOUTHERN SURVEYOR in the Bass Strait, Coral Sea and others from 2008011 to 2010-10-31 (NODC Accession 0115181)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0115181 includes chemical, meteorological, physical and underway - surface data collected from SOUTHERN SURVEYOR in the Bass Strait, Coral Sea, Great...

  6. Partial pressure (or fugacity) of carbon dioxide, salinity and other variables collected from Surface underway observations using Barometric pressure sensor, Carbon dioxide (CO2) gas analyzer and other instruments from Trans Future 5 in the Bass Strait, Coral Sea and others from 2012-01-16 to 2012-12-07 (NCEI Accession 0144347)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0144347 includes Surface underway data collected from Trans Future 5 in the Bass Strait, Coral Sea, East China Sea (Tung Hai), Inland Sea (Seto...

  7. Partial pressure (or fugacity) of carbon dioxide, salinity and other variables collected from Surface underway observations using Barometric pressure sensor, Carbon dioxide (CO2) gas analyzer and other instruments from Trans Future 5 in the Bass Strait, Coral Sea and others from 2006-06-27 to 2006-12-09 (NCEI Accession 0144357)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0144357 includes Surface underway data collected from Trans Future 5 in the Bass Strait, Coral Sea, North Pacific Ocean, Philippine Sea, Solomon Sea,...

  8. Partial pressure (or fugacity) of carbon dioxide, salinity and other variables collected from underway - surface observations using Carbon dioxide (CO2) gas analyzer, Shower head chamber equilibrator for autonomous carbon dioxide (CO2) measurement and other instruments from the Natalie Schulte in the Bass Strait, North Pacific Ocean and others from 2010-10-01 to 2012-06-21 (NODC Accession 0108233)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0108233 includes chemical, meteorological, physical and underway - surface data collected from Natalie Schulte in the Bass Strait, North Pacific...

  9. Contribution à la conception de circuits intégrés analogiques en technologie CMOS basse tension pour application aux instruments d'observation de la Terre

    OpenAIRE

    Standarovski, Denis

    2005-01-01

    Le présent mémoire de thèse s'inscrit dans la problématique d'intégration de chaînes pour traitement du signal vidéo issu d'un capteur CCD dédiées aux instruments d'observation de la Terre. La solution présentée à travers cette étude consiste à concevoir des circuits intégrés spécifiques (ASIC) analogiques avec des technologies CMOS sub-microniques basse-tension, principalement développées pour les circuits numériques complexes. Dans une première partie, nous présentons le contexte de l'étude...

  10. Implementation of a non-lethal biopsy punch monitoring program for mercury in smallmouth bass, Micropterus dolomieu Lacepède, from the Eleven Point River, Missouri.

    Science.gov (United States)

    Ackerson, J R; McKee, M J; Schmitt, C J; Brumbaugh, W G

    2014-02-01

    A non-lethal biopsy method for monitoring mercury (Hg) concentrations in smallmouth bass (Micropterus dolomieu; smallmouth) from the Eleven Point River in southern Missouri USA was evaluated. A biopsy punch was used to remove a muscle tissue plug from the area immediately below the anterior dorsal fin of 31 smallmouth. An additional 35 smallmouth (controls) were held identically except that no tissue plug was removed. After sampling, all fish were held in a concrete hatchery raceway for 6 weeks. Mean survival at the end of the holding period was 97 % for both groups. Smallmouth length, weight and Fulton's condition factor at the end of the holding period were also similar between plugged and non-plugged controls, indicating that the biopsy procedure had minimal impact on growth under these conditions. Tissue plug Hg concentrations were similar to smallmouth Hg data obtained in previous years by removing the entire fillet for analysis. PMID:24196376

  11. Production vivrière et accès à la terre dans un village de basse Côte d'Ivoire

    OpenAIRE

    Biarnès, Anne; Colin, Jean-Philippe

    1987-01-01

    Cet article présente les systèmes de culture vivriers pratiqués dans un village en basse Côte d'Ivoire et quelques-uns de leurs déterminants. Sont d'abord évoquées les modalités de l'insertion spatiale des cultures vivrières dans l'agriculture de plantation; puis, après la présentation d'une typologie des systèmes de culture vivriers, sont analysées la fonction économique des principales cultures (autoconsommation ou vente) et l'incidence des contraintes culturales et foncières sur la product...

  12. Implementation of a non-lethal biopsy punch monitoring program for mercury in smallmouth bass, Micropterus dolomieu Lacepède, from the Eleven Point River, Missouri

    Science.gov (United States)

    Ackerson, J.R.; Schmitt, C. J.; McKee, M.J.; Brumbaugh, W.G.

    2013-01-01

    A non-lethal biopsy method for monitoring mercury (Hg) concentrations in smallmouth bass (Micropterus dolomieu; smallmouth) from the Eleven Point River in southern Missouri USA was evaluated. A biopsy punch was used to remove a muscle tissue plug from the area immediately below the anterior dorsal fin of 31 smallmouth. An additional 35 smallmouth (controls) were held identically except that no tissue plug was removed. After sampling, all fish were held in a concrete hatchery raceway for 6 weeks. Mean survival at the end of the holding period was 97 % for both groups. Smallmouth length, weight and Fulton’s condition factor at the end of the holding period were also similar between plugged and non-plugged controls, indicating that the biopsy procedure had minimal impact on growth under these conditions. Tissue plug Hg concentrations were similar to smallmouth Hg data obtained in previous years by removing the entire fillet for analysis.

  13. Analysis of EST database for two subspecies of largemouth bass%大口黑鲈两个亚种EST数据库分析

    Institute of Scientific and Technical Information of China (English)

    景燕娟; 白俊杰; 李胜杰; 于凌云; 蔡磊

    2012-01-01

    应用新一代高通量测序技术Roche 454对大口黑鲈(Micropterus salmoides)北方亚种和佛罗里达亚种进行转录组测序并建立ESTs数据库,结果得到北方亚种ESTs序列468 671条,佛罗里达亚种ESTs序列332 322条,两亚种ESTs序列的平均长度分别为306.5bp和304.4bp。将得到的高质量序列进行拼接,大口黑鲈北方亚种和佛罗里达亚种共得到contig序列总数分别为42 056条和35 743条,平均长度分别为612.6bp和588.2bp。将大口黑鲈北方亚种和佛罗里达亚种的数据库合并,通过与蛋白数据库比对后,共78 938条EST序列被注释,根据Gene Ontology(GO)信息,对序列按照分子功能、细胞组成、生物学过程进行分类。通过对大口黑鲈合并的EST库信息进行大通量SSR和SNP位点的发掘,发现了含SSRs和SNPs的序列分别为25 469和8 547条。从EST数据库中随机选取75条contigs进行北方亚种和佛罗里达亚种的序列比较,结果表明两个亚种EST序列同源性为99.2%。%In this study,Roche 454 high-throughput technology was used to conduct transcriptome sequencing and establish ESTs database of the largemouth bass northern subspecies and Florida subspecies.The results showed that a total of 468 671 and 332 322 ESTs were generated from northern subspecies and Florida subspecies.The average length was 306.5 bp and 304.4 bp,respectively.Assembly of the largemouth bass northern subspecies and Florida subspecies' high quality ESTs resulted in 42 056 and 35 743 contigs.The average length was 612.6 bp and 588.2 bp,respectively.EST database of largemouth bass northern subspecies and Florida subspecies was merged and then compared with known protein databases.78 938 EST sequences in total were annotated.By exploring the merged largemouth bass EST library,over 25 469 and 8 547 putative SSRs and SNPs were identified.Comparison of the northern subspecies and Florida subspecies sequence was made by randomly selecting 75 contigs from

  14. Anoplastie périnéale simple pour le traitement des malformations anorectales basses chez l'adulte, à propos de deux cas

    OpenAIRE

    Abdelmoughit ECHCHAOUI; Benyachou, Malika; Hafidi, Jawad; Fathi, Nahed; Mohammadine, Elhamid; ELmazouz, Samir; Gharib, Nour-eddine; Abbassi, Abdellah

    2014-01-01

    Les malformations anorectales chez l'adulte sont des anomalies congénitales rares du tube digestif qui prédominent chez le sexe féminin. Notre étude porte sur deux observations de malformation anorectale basses vues et traitées au stade adulte par les 2 équipes (plasticiens et viscéralistes) à l'Hôpital Avicenne à Rabat. Il s'agit d'un homme de 24 ans avec une dyschésie anale l'autre cas est une femme de 18 ans avec une malformation anovulvaire Les caractéristiques cliniques combinées avec le...

  15. Isolation of TDA-producing Phaeobacter strains from sea bass larval rearing units and their probiotic effect against pathogenic Vibrio spp. in Artemia cultures.

    Science.gov (United States)

    Grotkjær, Torben; Bentzon-Tilia, Mikkel; D'Alvise, Paul; Dourala, Nancy; Nielsen, Kristian Fog; Gram, Lone

    2016-05-01

    Fish-pathogenic Vibrio can cause large-scale crashes in marine larval rearing units and, since the use of antibiotics can result in bacterial antibiotic resistance, new strategies for disease prevention are needed. Roseobacter-clade bacteria from turbot larval rearing facilities can antagonize Vibrio anguillarum and reduce mortality in V. anguillarum-infected cod and turbot larvae. In this study, it was demonstrated that antagonistic Roseobacter-clade bacteria could be isolated from sea bass larval rearing units. In addition, it was shown that they not only antagonized V. anguillarum but also V. harveyi, which is the major bacterial pathogen in crustaceans and Mediterranean sea bass larvae cultures. Concomitantly, they significantly improved survival of V. harveyi-infected brine shrimp. 16S rRNA gene sequence homology identified the antagonists as Phaeobacter sp., and in silico DNA-DNA hybridization indicated that they could belong to a new species. The genomes contained genes involved in synthesis of the antibacterial compound tropodithietic acid (TDA), and its production was confirmed by UHPLC-TOFMS. The new Phaeobacter colonized live feed (Artemia) cultures and reduced Vibrio counts significantly, since they reached only 10(4)CFUmL(-1), as opposed to 10(8)CFUmL(-1) in non-Phaeobacter treated controls. Survival of V. anguillarum-challenged Artemia nauplii was enhanced by the presence of wild type Phaeobacter compared to challenged control cultures (89±1.0% vs 8±3.2%). In conclusion, TDA-producing Phaeobacter isolated from Mediterranean marine larviculture are promising probiotic bacteria against pathogenic Vibrio in crustacean live-feed cultures for marine fish larvae. PMID:26922490

  16. Locomotory behaviour and post-exercise physiology in relation to swimming speed, gait transition and metabolism in free-swimming smallmouth bass (Micropterus dolomieu).

    Science.gov (United States)

    Peake, Stephan J; Farrell, Anthony P

    2004-04-01

    We examined swimming behaviour, gait recruitment and post-exercise muscle glycogen, muscle lactate, plasma lactate and oxygen consumption in smallmouth bass (Micropterus dolomieu; 24-38 cm fork length) that voluntarily ascended a 25 m raceway against water velocities ranging from 40 to 120 cm s(-1). Physiological parameters were referenced to additional measurements made following exhaustive exercise in a static tank and aerobic exercise in a swim tunnel. Maximum speeds maintained exclusively using a steady gait in the raceway ranged from 53.6 to 97.3 cm s(-1) and scaled positively with fish length. Minimum swimming speeds maintained exclusively through recruitment of an unsteady gait were also positively correlated to fish length and ranged from 81.4 to 122.9 cm s(-1). Fish switched between steady and unsteady swimming at intermediate speeds. Smallmouth bass always maintained a positive ground speed in the raceway; however, those that primarily swam using a steady gait to overcome low to moderate water velocities (20-50 cm s(-1)) maintained mean ground speeds of approximately 20 cm s(-1). By contrast, mean ground speeds of fish that primarily recruited an unsteady locomotory gait increased significantly with water velocity, which resulted in an inverse relationship between exercise intensity and duration. We interpret this behaviour as evidence that unsteady swimming was being fuelled by the limited supply of anaerobic substrates in the white muscle. This hypothesis is supported by the fact that unsteady swimming fish showed significantly lower muscle glycogen levels, higher lactate concentrations (muscle and plasma) and higher post-exercise oxygen consumption rates compared with fish that used a steady gait. The reduction in passage time achieved by fish using an unsteady gait allowed them to ascend the raceway with relatively minor post-exercise metabolic imbalances, relative to individuals chased to exhaustion.

  17. The technique of autoradiography at very low temperature; Technique d'autoradiographie a tres basse temperature

    Energy Technology Data Exchange (ETDEWEB)

    Pellerin, P. [Commissariat a l' Energie Atomique, Saclay (France).Centre d' Etudes Nucleaires

    1959-07-01

    ferme. Le developpement a lieu a temperature ordinaire et la sensibilite de l'emulsion n'est pas appreciabdement modifiee. Cette technique permet de maintenir le bloc tres au-dessous de la temperature de congelation, des la mort de l'animal jusqu'au developpement; toute diffusion chimique est supprimee et la repartition de la substance radioactive saisie sur l'autoradiographie a l'instant exact de la mort par congelation. Des fraisages successifs permettent d'etudier la repartition de la substance radioactive, a toutes les profondeurs. L'absence d'effets pseudo-radiographiques est totale. Enfin, le renforcement des couleurs naturelles des elements anatomiques par cette tres basse temperature est considerable, ce qui permet de realigner l'autoradiographie en couleurs de le coupe processus de coloration. (auteur)

  18. The relative importance of oceanic nutrient inputs for Bass Harbor Marsh Estuary at Acadia National Park, Maine

    Science.gov (United States)

    Huntington, Thomas G.; Culbertson, Charles W.; Fuller, Christopher; Glibert, Patricia; Sturtevant, Luke

    2014-01-01

    The U.S. Geological Survey and Acadia National Park (ANP) collaborated on a study of nutrient inputs into Bass Harbor Marsh Estuary on Mount Desert Island, Maine, to better understand ongoing eutrophication, oceanic nutrient inputs, and potential management solutions. This report includes the estimation of loads of nitrate, ammonia, total dissolved nitrogen, and total dissolved phosphorus to the estuary derived from runoff within the watershed and oceanic inputs during summers 2011 and 2012. Nutrient outputs from the estuary were also monitored, and nutrient inputs in direct precipitation to the estuary were calculated. Specific conductance, water temperature, and turbidity were monitored at the estuary outlet. This report presents a first-order analysis of the potential effects of projected sea-level rise on the inundated area and estuary volume. Historical aerial photographs were used to investigate the possibility of widening of the estuary channel over time. The scope of this report also includes analysis of sediment cores collected from the estuary and fringing marsh surfaces to assess the sediment mass accumulation rate. Median concentrations of nitrate, ammonium, and total dissolved phosphorus on the flood tide were approximately 25 percent higher than on the ebb tide during the 2011 and 2012 summer seasons. Higher concentrations on the flood tide suggest net assimilation of these nutrients in biota within the estuary. The dissolved organic nitrogen fraction dominated the dissolved nitrogen fraction in all tributaries. The median concentration of dissolved organic nitrogen was about twice as high on the on the ebb tide than the flood tide, indicating net export of dissolved organic nitrogen from the estuary. The weekly total oceanic inputs of nitrate, ammonium, and total dissolved phosphorus to the estuary were usually much larger than inputs from runoff or direct precipitation. The estuary was a net sink for nitrate and ammonium in most weeks during both

  19. Shelf-life extension of refrigerated sea bass slices wrapped with fish protein isolate/fish skin gelatin-ZnO nanocomposite film incorporated with basil leaf essential oil.

    Science.gov (United States)

    Arfat, Yasir Ali; Benjakul, Soottawat; Vongkamjan, Kitiya; Sumpavapol, Punnanee; Yarnpakdee, Suthasinee

    2015-10-01

    Microbiological, chemical and sensory changes of sea bass slices wrapped with fish protein isolate (FPI)/fish skin gelatin (FSG) films incorporated with 3 % ZnO nanoparticles (ZnONP) (w/w, based on protein content) and 100 % basil leaf essential oil (BEO) (w/w, based on protein content) during storage of 12 days at 4 °C were investigated. Sea bass slices wrapped with FPI/FSG-ZnONP-BEO film had the lowest growth of psychrophilic bacteria, lactic acid bacteria and spoilage microorganisms including Pseudomonas , H2S-producing bacteria and Enterobacteriaceae throughout storage of 12 days in comparison with those wrapped with FPI/FSG-BEO, FPI/FSG-ZnONP, FPI/FSG film, polypropylene film (PP film) and the control (without wrapping), respectively (P control (6 days) (P < 0.05). PMID:26396365

  20. Record Of Both Tectonic Related Vertical Motions and Global Sea Level Rise by Marine Terraces along an Active Arc Volcano. Example of Basse-Terre, Lesser Antilles (French West-Indies).

    Science.gov (United States)

    Fabre, M.; Moysan, M.; Graindorge, D.; Jean-Frederic, L.; Philippon, M. M.; Marcaillou, B.; Léticée, J. L.

    2015-12-01

    Volcano-tectonic history of the Caribbean plate provides direct insight onto the dynamic of the North American Plate westward subduction. Basse-Terre Island is a volcanic chain that belongs to the Lesser Antilles active volcanic arc with a southward decreasing age of volcanism from 3 Ma to present day.We investigate records of vertical motion along Basse-Terre through a morphostructural analysis of the Pleistocene-Holocene shallow-water carbonate platforms and associated terraces that surround Basse-Terre Island. This study is based on new high-resolution bathymetric and dense seismic data acquired during the GEOTREF oceanographic survey (2015, February). Our bathymetric and topographic Digital Terrain Model together with the "Litto3D" Lidar data (IGN/SHOM) images the island topography and the platform bathymetry to a depth of 200m with horizontal and vertical resolutions of 5m and ~cm respectively. This detailed study highlights the morphostructure of terraces built during the last transgression in order to identify and quantify their vertical motions. We analyze inherited morphology and structures of the forearc that affect the platform to discuss effects of the regional tectonics context. A particular emphasis is put on the influence of the NW-SE arc parallel transtensive Montserrat-Bouillante fault system onto the platform geometry. At last, the distribution of Basse-Terre terraces is compared with terraces distribution around other Lesser Antilles island and the Bahamas stable margin platform. We aim at discriminating the influence of the Pleistocene global sea-level rise from the one of tectonic vertical deformations.

  1. Regulation of endocrine and paracrine sources of insulin-like growth factors and growth hormone receptor during compensatory growth in hybrid striped bass (Morone chrysops x Morone saxatilis)

    DEFF Research Database (Denmark)

    Picha, Matthew E; Turano, Marc J; Tipsmark, Christian K;

    2008-01-01

    Compensatory growth (CG) is a period of growth acceleration that exceeds normal rates after animals are alleviated of certain growth-stunting conditions. In hybrid striped bass (HSB, Morone chrysops x M. saxatilis), 3 weeks of complete feed restriction results in a catabolic state that, when...... liver production, rather than as a fraction of total RNA, may be a more biologically appropriate method of quantifying hepatic gene expression when using real-time PCR....

  2. Influence of temperature in thermal and oxidative stress responses in estuarine fish.

    Science.gov (United States)

    Madeira, D; Narciso, L; Cabral, H N; Vinagre, C; Diniz, M S

    2013-10-01

    The influence of increasing temperatures in thermal and oxidative stress responses were studied in the muscle of several estuarine fish species (Diplodus vulgaris, Diplodus sargus, Dicentrarchus labrax, Gobius niger and Liza ramada). Selected fish were collected in July at the Tagus estuary (24±0.9°C; salinity of 30±4‰; pH=8). Fish were subjected to a temperature increase of 1°C.h(-1) until they reached their Critical Thermal Maximum (CTMax), starting at 24°C (control temperature). Muscle samples were collected during the trial and results showed that oxidative stress biomarkers are highly sensitive to temperature. Results from stress oxidative enzymes show alterations with increasing temperature in all tested species. Catalase (CAT; EC 1.11.1.6) activity significantly increased in L. ramada, D. labrax and decreased in D. vulgaris. Glutathione S-transferase (GST; EC 2.5.1.18) activity increased in L. ramada, D. sargus, D. vulgaris, and D. labrax. In G. niger it showed a cycle of increase-decrease. Lipid peroxidation (LPO) increased in L. ramada, D. sargus and D. labrax. With respect to correlation analysis (Pearson; Spearman r), the results showed that oxidation products and antioxidant defenses were correlated in L. ramada (LPO-CAT and LPO-GST, D. sargus (LPO-CAT), and D. labrax (LPO-CAT). Oxidative biomarkers were correlated with thermal stress biomarker (Hsp70) in L. ramada (CAT-Hsp70), D. vulgaris (LPO-Hsp70), D. labrax (GST-Hsp70) and G. niger (LPO-Hsp70). In conclusion, oxidative stress does occur with increasing temperatures and there seems to be a relation between thermal stress response and oxidative stress response. The results suggest that oxidative stress biomarkers should be applied with caution, particularly in field multi-species/multi-environment studies. PMID:23774589

  3. Progress On Intestinal Oligopeptide Transporter PepT1 of Fishes%鱼类肠道小肽转运载体PepT1的研究进展

    Institute of Scientific and Technical Information of China (English)

    叶万里; 李英文

    2009-01-01

    从生理特性,cDNA序列和蛋白质分子结构,组织分布与表达这三个方面叙述了欧洲鳗鲡(Anguilla anguilla),罗非鱼(Oreochromis mossambicus),rockfish(Sebastes caurinus),银鱼(Chionodraco hamatus),斑马鱼(Danio rerio),亚洲泥鳅(Misgurnus anguillicaudatus),大西洋鳕鱼(Gadus morhua)和欧洲黑鲈(Dicentrarchus labrax)中有关肠道Ⅰ型肽转运载体(PepT1)方面的研究进展情况.

  4. FITOPLANKTONSKI SASTAV NA UZGAJALIŠTU RIBA U UVALI MASLINOVA (OTOK BRAČ)

    OpenAIRE

    SKEJIĆ, Sanda; Marasović, Ivona; NINČEVIĆ GLADAN, Živana

    2012-01-01

    Cilj ovog rada je utvrditi sastav fitoplanktona na uzgajalištu komarči (Sparus aurata) i lubina (Dicentrarchus labrax) u srednjem Jadranu. Istraživanje je provedeno u razdoblju od rujna 2005. do rujna 2006. godine na postaji smještenoj u uvali Maslinova na otoku Braču. Promatrajući cijelo istraživano razdoblje, dijatomeje su općenito prevladavale svojom brojnošću, dok su dinoflagelati bili brojniji u lipnju. Broj vrsta dijatomeja i dinoflagelata kroz istraživano razdoblje bio je sličan. Z...

  5. SASTAV FITOPLANKTONA NA UZGAJALIŠTU RIBA I ŠKOLJKAŠA U UVALI KALDONTA (OTOK CRES)

    OpenAIRE

    Tomec, M.

    2004-01-01

    Uvala Kaldonta nalazi se na jugozapadnoj obali otoka Cresa u Lošinjskom kanalu na području bez većeg utjecaja opće morske struje. U uvali su postavljena 44 plutajuća kaveza veličine 5x10 metara u kojima se uspješno proizvodi oko 70 tona morske ribe: komarča (Sparus aurata), lubin (Dicentrarchus labrax), pic (Diplodus puntazzo) i zubatac (Dentex dentex). Uz neke fizikalnokemijske pokazatelje (temperatura mora, prozirnost, salinitet), posebna je pozornost posvećena kvalitativnom sastavu mrežnog...

  6. KVALITETA MESA LUBINA TIJEKOM POHRANE NA LEDU

    OpenAIRE

    Bojanić, Krunoslav; Kozačinski, Lidija; Filipović, Ivana; Zdolec, Nevijo; Njari, Bela

    2009-01-01

    Cilj ovog rada bio je odrediti održivost mesa lubina tijekom njegove pohrane na ledu u trajanju od 12 dana pracenjem senzornih, fizikalno-kemijskih i mikrobioloških parametara kakvoce i higijenske ispravnosti. U tu je svrhu pretraženo 30 lubina 2., 4., 6., 9. i 12. dana pohrane na ledu na 3º C. Senzorna ocjena kvalitete ocijenjena je Quality Index Method testom (QIM) izra!enom za lubina, Dicentrarchus labrax (Icelandic Fisheries Laboratories) i Market testom. Uzorci ri...

  7. The use of high rate algal ponds for the treatment of marine effluent from a recirculating fish rearing system

    OpenAIRE

    Pagand, Pascal; Blancheton, Jean-Paul; Lemoalle, J; Casellas, C.

    2000-01-01

    A high rate algal pond (HRAP) system was used to treat effluent from a recirculating sea water aquaculture system in southern France. Dicentrarchus labrax L. were farmed at a high density, with effluents containing an average of 10 mg L-1 dissolved inorganic nitrogen (DIN) and 1.3 mg L-1 reactive phosphorus (RP). On a yearly basis, the algal pond removed 59% of the dissolved nitrogen and 56% of the phosphorus input, which was converted into 3.3 kg DW m(-2) algae. Green macroalgae were dominan...

  8. 低音提琴的演奏及其效果提升的路径分析%An Analysis of the Performance and Effect Promotion Path of Double Bass

    Institute of Scientific and Technical Information of China (English)

    张晗

    2014-01-01

    低音提琴声部是管弦乐队中的一个重要的和声基础,整个管弦乐队演奏出的美妙的、立体式的音响效果,不单单是指挥、高中音乐器、打击乐器等对作品的音乐表达,而是低音提琴声部与其他高中音乐器、其他低音声部乐器等共同创造出来的。如果低音提琴的演奏效果不到位,将会直接影响整个乐队对于演奏作品的表达。从低音提琴的演奏动作及其规范问题、气息的调整与多种弓法的熟练掌握、演奏者之间的交流和专业沟通三个方面分析强化基础训练与提升演奏效果的关系,指出感知与领会乐曲是增强乐曲演奏感染力的重要环节,并从把握乐曲本身所反映的内涵和情感、将演奏者的感情融入到作品中去、增强团队精神和协奏意识等方面进行论述。%Double bass is an important basic harmony in an orchestra .A wonderful and three-dimensional sound from the whole orchestra is obtained not only by the conductor ,the treble and alto musical instru-ment and percussion instrument ,but also by a combination of the double bass with other treble ,alto and bass musical instruments .It is believed that if the double bass is not performed in place ,expression of the works will be directly affected .In this article ,the relationship between strengthening basic training and upgrading the performance effect is analyzed from three aspects :performance gesture and regulatory issues in the double bass ,breath control and proficiency in a variety of bowing and professional communication between performers .Perception and understanding play an important role in enhancement of music appeal . Moreover ,how to accurately reflect meaning and emotion of works ,how to integrate performer's senti-ment to the work and how to enhance team spirits and concerto consciousness are also discussed .

  9. Condition of larval and early juvenile Japanese temperate bass Lateolabrax japonicus related to spatial distribution and feeding in the Chikugo estuarine nursery ground in the Ariake Bay, Japan

    Science.gov (United States)

    Islam, Md. Shahidul; Hibino, Manabu; Nakayama, Kouji; Tanaka, Masaru

    2006-02-01

    The present study investigates feeding and condition of larval and juvenile Japanese temperate bass Lateolabrax japonicus in relation to spatial distribution in the Chikugo estuary (Japan). Larvae were collected in a wide area covering the nursery grounds of the species in 2002 and 2003. Food habits of the fish were analysed by examining their gut contents. Fish condition was evaluated by using morphometric (the length-weight relationship and condition factor) and biochemical (the RNA:DNA ratio and other nucleic acid based parameters) indices and growth rates. The nucleic-acid contents in individually frozen larvae and juveniles were quantified by standard fluorometric methods. Two distinct feeding patterns, determined by the distribution of prey copepods, were identified. The first pattern showed dependence on the calanoid copepod Sinocalanus sinensis, which was the single dominant prey in low-saline upper river areas. The second pattern involved a multi-specific dietary habit mainly dominated by Acartia omorii, Oithona davisae, and Paracalanus parvus. As in the gut contents analyses, two different sets of values were observed for RNA, DNA, total protein, growth rates and for all the nucleic acid-based indices: one for the high-saline downstream areas and a second for the low-saline upstream areas, which was significantly higher than the first. The proportion of starving fish was lower upstream than downstream. Values of the allometric coefficient ( b) and the condition factor ( K) obtained from the length-weight relationships increased gradually from the sea to the upper river. Clearly, fish in the upper river had a better condition than those in the lower estuary. RNA:DNA ratios correlated positively with temperature and negatively with salinity. We hypothesise that by migration to the better foraging grounds of the upper estuary (with higher prey biomass, elevated temperature and reduced salinity), the fish reduce early mortality and attain a better condition

  10. Toxic effects of harmful benthic dinoflagellate Ostreopsis ovata on invertebrate and vertebrate marine organisms.

    Science.gov (United States)

    Faimali, Marco; Giussani, Valentina; Piazza, Veronica; Garaventa, Francesca; Corrà, Christian; Asnaghi, Valentina; Privitera, Davide; Gallus, Lorenzo; Cattaneo-Vietti, Riccardo; Mangialajo, Luisa; Chiantore, Mariachiara

    2012-05-01

    Harmful benthic microalgae blooms are an emerging phenomenon causing health and economic concern, especially in tourist areas. This is the case of the Mediterranean Sea, where Ostreopsis ovata blooms occur in summer, with increasing regularity. Ostreopsis species produce palytoxin (PTX) and analogues, and a number of deaths directly associated with the ingestion of PTX contaminated seafood have been reported. PTX is considered one of the most toxic molecules occurring in nature and can provoke severe and sometimes lethal intoxications in humans. So far in temperate areas, O. ovata blooms were reported to cause intoxications of humans by inhalation and irritations by contact. In addition, invertebrate mass mortalities have been reported, possibly linked to O. ovata blooms, although other causes cannot be ruled out, such as oxygen depletion or high seawater temperature. In order to improve our knowledge about the direct toxicity of this species on invertebrate and vertebrate marine organisms, we performed an ecotoxicological screening to investigate the toxic effects of different concentrations of O. ovata (cultured in the laboratory and sampled in the field during blooms) on crustaceans and fish as model organisms. Artemia salina, Tigriopus fulvus, and Amphibalanus amphitrite larvae and juveniles of the sea bass Dicentrarchus labrax were used as model species. Toxic effects associated with cultured O. ovata cells were investigated using a crossed design: testing two different temperatures (20 and 25 °C), four different cell concentrations, and four treatments (untreated O. ovata culture, filtered and resuspended algal cells, growth medium devoid of algal cells, and sonicated algal cells). The results indicate that the toxicity of cultured O. ovata is related to the presence of living O. ovata cells, and that this effect is amplified by temperature. Furthermore, both tests with laboratory cultured algae and field sampled cells pointed out that A. salina is the most

  11. PHYTOPLANKTON COMPOSITION AT THE FISH AND SHELLFISH FARM IN THE KALDONTA BAY (CRES ISLAND

    Directory of Open Access Journals (Sweden)

    Marija Tomec

    2004-12-01

    Full Text Available The Kaldonta Bay is situated at the south–western coast of the Cres island in the Lošinj channel, rather protected from larger influence of general sea water current. In the Bay there are installed 44 floating cages of 5 by 10 m dimensions. The cages are used for the culture of about 70 tons of sea water fish: gilthead sea bream (Sparus aurata, sea bass (Dicentrarchus labrax, sharp–snouted sparus (Diplodus puntazzo and dentex (Dentex dentex. Besides some physico–chemical parameters (sea water temperature, transparence and salinity, special attention has been paid to the qualitative composition of net phytoplankton. Investigations were performed in the period of May, September and December 2003 and February 2004 at five locations in the Kaldonta Bay (Figure 1 at the depths of 0.5 m, 5 m, 10 m and 1 m from the bottom. According to the physico–chemical parameters, sea water temperature was influenced by the temperature of the environment, and the transparence suggested to the oligotrophic situation in the investigated aquatorium. Qualitative composition of net phytoplankton comprised 161 microphytic species belonging to the systematic compartments of Cyanobacteria, Chrysophyta and Dinophyta (Table 1. The most numerous algal group were diatoms or Bacillarophyceae (98 species or 61%, with relative frequencies of species from 1 to 7. Taxonomic composition of diatoms showed Chaetoceros–Rhizosolenia (Proboscia to be the dominant community. Diatom species was the most abundant in late autumn period (beginning of December. The second most important comparatment were Dinophyta (55 species or 34.1%, with the dominant genera Ceratium and Protoperidinium. During the investigation, the representatives of Dinophyta did not show large variety of species in the water column. Relative frequency of the species was 1, rarely 2 and 3. Dinophyts were the most abundant in September. From Cyanobacteria (5 species or 3.1% only filamentous algae were determined

  12. Immunological role of C4 CC chemokine-1 from snakehead murrel Channa striatus.

    Science.gov (United States)

    Bhatt, Prasanth; Kumaresan, Venkatesh; Palanisamy, Rajesh; Chaurasia, Mukesh Kumar; Gnanam, Annie J; Pasupuleti, Mukesh; Arockiaraj, Jesu

    2014-02-01

    In this study, we have reported a cDNA sequence of C4 CC chemokine identified from snakehead murrel (also known as striped murrel) Channa striatus (named as CsCC-Chem-1) normalized cDNA library constructed by Genome Sequencing FLX™ Technology (GS-FLX™). CsCC-Chem-1 is 641 base pairs (bp) long that contain 438 bp open reading frame (ORF). The ORF encodes a polypeptide of 146 amino acids with a molecular mass of 15 kDa. The polypeptide contains a small cytokine domain at 30-88. The domain carries the CC motif at Cys(33)-Cys(34). In addition, CsCC-Chem-1 consists of another two cysteine residues at C(59) and C(73), which, together with C(33) and C(34), make CsCC-Chem-1 as a C4-CC chemokine. CsCC-Chem-1 also contains a 'TCCT' motif at 32-35 as CC signature motif; this new motif may represent new characteristic features, which may lead to some unknown function that needs to be further focused on. Phylogenitically, CsCC-Chem-1 clustered together with CC-Chem-1 from rock bream Oplegnathus fasciatus and European sea bass Dicentrarchus labrax. Significantly (P<0.05) highest gene expression was noticed in spleen and is up-regulated upon fungus (Aphanomyces invadans), bacteria (Aeromonas hydrophila) and virus (poly I:C) infection at various time points. The gene expression results indicate the influence of CsCC-Chem-1 in the immune system of murrel. Overall, the gene expression study showed that the CsCC-Chem-1 is a capable gene to increase the cellular response against various microbial infections. Further, we cloned the coding sequence of CsCC-Chem-1 in pMAL vector and purified the recombinant protein to study the functional properties. The cell proliferation activity of recombinant CsCC-Chem-1 protein showed a significant metabolic activity in a concentration dependent manner. Moreover, the chemotaxis assay showed the capability of recombinant CsCC-Chem-1 protein which can induce the migration of spleen leukocytes in C. striatus. However, this remains to be verified

  13. Environmental factors structuring fish composition and assemblages in a small macrotidal estuary (eastern English Channel)

    Science.gov (United States)

    Selleslagh, Jonathan; Amara, Rachid

    2008-09-01

    The fish assemblage structure was analyzed along an estuarine gradient of a small macrotidal estuary (the Canche, France). Fishes were collected every two months between May 2006 and July 2007 from 12 sampling stations using a 1.5-m beam trawl with a 5 mm mesh size in the cod end. To complement this information, sampling was also performed using 15-m fyke nets (8 mm mesh size in the cod end). For each sample, abiotic (temperature, salinity, pH, oxygen, turbidity, river flow, wind speed and depth) and biotic (macro crustacean species abundances) were recorded. Throughout the study, 28 fish species belonging to 20 families were collected. Fish catches were dominated by juveniles, especially Young-Of-the-Year (YOY) for the majority of the species. According to the Index of Relative Importance (IRI), common goby Pomatoschistus microps, flounder Platichtys flesus, sprat Sprattus sprattus, sea-bass Dicentrarchus labrax and plaice Pleuronectes platessa were the most abundant species, together accounting for 99.2% of the total IRI. Estuarine residents (ER = 66.2%) and marine juvenile migrants species (MJ = 31.4%) were the most important ecological guilds. The structure of the fish assemblage and its relationship to environmental variables was examined using multivariate techniques. Cluster and non-metric multidimensional scaling (nMDS) analysis defined six distinct groups in the Canche estuary, which are discriminated by specific species (SIMPER). Spatio-temporal variations in fish assemblage structure reflect the density peaks of the most abundant species. Spearman rank correlations and canonical correspondence analysis (CCA) showed that among the ten environmental variables examined, temperature, salinity and Crangon crangon (a potential predator for YOY fish or prey for older ones) are the three most important factors influencing fish species richness and abundances. Our observations reinforce the idea that certain fish species may have different life history styles in

  14. Critique and sensitivity analysis of the compensation function used in the LMS Hudson River striped bass models. Environmental Sciences Division publication No. 944

    Energy Technology Data Exchange (ETDEWEB)

    Van Winkle, W.; Christensen, S.W.; Kauffman, G.

    1976-12-01

    The description and justification for the compensation function developed and used by Lawler, Matusky and Skelly Engineers (LMS) (under contract to Consolidated Edison Company of New York) in their Hudson River striped bass models are presented. A sensitivity analysis of this compensation function is reported, based on computer runs with a modified version of the LMS completely mixed (spatially homogeneous) model. Two types of sensitivity analysis were performed: a parametric study involving at least five levels for each of the three parameters in the compensation function, and a study of the form of the compensation function itself, involving comparison of the LMS function with functions having no compensation at standing crops either less than or greater than the equilibrium standing crops. For the range of parameter values used in this study, estimates of percent reduction are least sensitive to changes in YS, the equilibrium standing crop, and most sensitive to changes in KXO, the minimum mortality rate coefficient. Eliminating compensation at standing crops either less than or greater than the equilibrium standing crops results in higher estimates of percent reduction. For all values of KXO and for values of YS and KX at and above the baseline values, eliminating compensation at standing crops less than the equilibrium standing crops results in a greater increase in percent reduction than eliminating compensation at standing crops greater than the equilibrium standing crops.

  15. ANTILISTERIAL ACTYVITY OF LACTIC ACID BACTERIA ISOLATED FROM GILTHEAD BREAMS AND SEA BASSES FILLETS PACKAGED MAP AGAINST PRIMITIVE STRAINS OF LISTERIA MONOCYTOGENES

    Directory of Open Access Journals (Sweden)

    M. Barile

    2011-04-01

    Full Text Available Listeria monocytogenes is the causative agent of listeriosis typically caused by ready-to-eat processed food that have a refrigerated shelf-life, but lightly preserved fish products also belong to a high-risk category. Aim of the work was to evaluate antimicrobial activity linked bacteriocin-producing of LAB isolated from gilthead breams and sea basses fillets packaged in modified atmospheres. Fifty-five LAB strains were screened against 21 strains of Listeria monocytogenes, 1 Listeria innocua held in the culture collection of Department of Zootechnical Sciences and Food Ispection (SIA and submitted to antagonistic activity using the spot on lawn and the agar well diffusion assay. Lactococcus lactis sub. lactis Sa31 was able to produce bacteriocin in agar and different broth medium. The bacteriocin man31 showed sensitivity to trypsin, pronase E and papain, inactivation at temperatures ≥ 100°C, bactericidal mode of action and antilisterial act, rapidly. The bacteriocin man31 caused a reduction of L. monocytogenes ½ c growth about log10 > 3 UFC/ml, when was applied on indicator strain at 20,480 AU/ml concentration, in vitro.

  16. Kinetic and physicochemical properties of brain acetylcholinesterase from the peacock bass (Cichla ocellaris) and in vitro effect of pesticides and metal ions.

    Science.gov (United States)

    Silva, Kaline Catiely Campos; Assis, Caio Rodrigo Dias; Oliveira, Vagne Melo; Carvalho, Luiz Bezerra; Bezerra, Ranilson Souza

    2013-01-15

    Brain acetylcholinesterase (AChE; EC 3.1.1.7) from peacock bass (Cichla ocellaris) was characterized and the effect of organophosphorus and carbamate pesticides as well as ions and heavy metals was evaluated. The kinetic parameters K(m) and V(max) were determined as 0.769 mM and 0.189 U/mg of protein respectively. Optimal pH and temperature were found to be 8.0 and 45°C. The enzyme retained approximately half of the activity after incubation at 50°C for 30 min. Total cholinesterase activity on brain of this species can be ascribed to AChE according to selective inhibitors analysis (neostigmine, eserine and BW284c5 reduced its activity whereas no effect was noticed for Iso-OMPA). Seven pesticides (five organophosphates: dichlorvos, diazinon, chlorpyrifos, temephos, tetraethyl pyrophosphate - TEPP and two carbamates: carbaryl and carbofuran) showed inhibitory effects on C. ocellaris AChE. However, the strongest effect was observed with carbofuran (IC(50)=0.21 μM and K(i)=2.57 × 10(-3) μM). The following ions (1 mM) showed to inhibit its activity (decrescent order): Hg(2+)>As(3+)>Cu(2+)>Zn(2+). EDTA(2-) did not affect enzyme activity. The present study provides assay conditions and data to suggest this enzyme as in vitro biomarker of organophosphorus and carbamate pesticides in routine environmental screening programs. PMID:23220411

  17. Diversity in destinations, routes and timing of small adult and sub-adult striped bass Morone saxatilis on their southward autumn migration

    Science.gov (United States)

    Mather, Martha E.; Finn, John T.; Pautzke, Sarah M.; Fox, Dewayne A.; Savoy, Tom; Brundage, Harold M.; Deegan, Linda A.; Muth, Robert M.

    2010-01-01

    Almost three-quarters of the 46 young adult and sub-adult striped bass Morone saxatilis that were acoustically tagged in Plum Island Estuary, Massachusetts, U.S.A., in the summer of 2006 were detected in one or more southern coastal arrays during their autumn migration. On the basis of the trajectories along which these M. saxatilis moved from feeding to overwintering areas, three migratory groups emerged. After leaving Plum Island Estuary, about half of the fish were detected only in a mid-latitude array, Long Island Sound. The other half of the tagged fish were detected during autumn and winter in a more southern array, the Delaware Estuary. This latter group of fish may have used two routes. Some travelled to the Delaware Estuary through Long Island Sound while other fish may have taken a second, more direct, coastal route that did not include Long Island Sound. Consequently, a seemingly homogeneous group of fish tagged at the same time in the same non-natal feeding location exhibited a diversity of southward movement patterns that could affect population-level processes. These three groups that differed in overwintering location and migration route could be movement contingents with migratory connectivity.

  18. Validation, use, and disadvantages of enzyme-linked immunosorbent assay kits for detection of cortisol in channel catfish, largemouth bass, red pacu, and golden shiners.

    Science.gov (United States)

    Sink, T D; Lochmann, R T; Fecteau, K A

    2008-03-01

    Measurement of cortisol response is an important tool to asses stress in fisheries research. Radioimmunoassay (RIA) is a common method for the measure of cortisol in fish. Use of enzyme-linked immunosorbent assays (ELISA) to detect cortisol would eliminate health hazards, costs of handling radioisotopes, and the short stability time associated with RIA. Enzyme-linked immunosorbent assays have been used for the determination of cortisol in several fish species. However, the ELISA method of cortisol determination in fish lacks proper validation testing. We conducted validation procedures for multiple commercial cortisol ELISA kits and compared the results to RIA. The assays were tested for four species: (1) channel catfish Ictalurus punctatus, (2) largemouth bass Micropterus salmoides, (3) red pacu Piaractus brachypomus, and (4) golden shiners Notemigonus crysoleucas. We evaluated the ELISA methods against RIA, and determined that at least one kit is suitable (accuracy: mean recovery of spiked samples, 102.8%; reproducibility: interassay coefficient of variation 0.96 for all species) for the measurement of cortisol response in fish and comparative determination of stress. All of the ELISA assay results varied by more than 10% from the cortisol concentrations detected by the RIA. The high variability of the kit results indicates that commercial ELISA kits could be utilized for qualitative determination of cortisol in fish, but should be fully validated in each laboratory for each species before being used for research. PMID:18649027

  19. A contribution to the theory of nuclear matter at low density; Contribution a la theorie de la matiere nucleaire a basse densite

    Energy Technology Data Exchange (ETDEWEB)

    Madan, L. M. [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires

    1961-07-01

    A combination of the variational method using special momentum conserving canonical transformations and the method of resummation of the infinite series of dominating terms in the low density limit is used to study a strongly interacting N particle system. Under the hypothesis that the excitation energy E{sub k} is convex and everywhere positive after the special canonical transformation minimising the average value of the energy has been performed, we have shown that the t-matrix obtained by the summation of the ascending ladders is free from all singularities. Some particular examples are studied in detail. (author) [French] Une combinaison de la methode variationnelle utilisant des transformations canoniques speciales conservant l'impulsion, et de la methode de resommation infinie des termes dominants a basse densite est utilisee pour etudier l'etat fondamental d'un systeme a N particules en interaction forte. Sous reserve de l'hypotnese que l'energie d'excitation E{sub k} est convexe et partout positive, nous avons demontre la regularite de la matrice t obtenue en resommant des echelles montantes une fois effectuee la transformation canonique speciale qui minimise la valeur moyenne de l'energie. Quelques exemples particuliers sont etudies en detail. (auteur)

  20. Glucose turnover in kelp bass (Paralabrax sp.): in vivo studies with [6-3H,6-14C]glucose

    International Nuclear Information System (INIS)

    [6-3H,6-14C]glucose was injected via an indwelling arterial cannula in free-swimming, fed, and fasted kelp bass to determine hepatic glucose production, peripheral glucose uptake, minimal glucose mass, mean transit time, and the percent of carbon recycling under the two different nutritional states. Mean plasma glucose levels remained unchanged in fed and fasted fish (48 +- 8 vs. 43 +- 8 mg/100 ml). During steady-state conditions, glucose replacement rates of fed and fasted fish determined with [6-3H]glucose are similar (0.035 +- 0.006 vs. 0.025 +- 0.003 mg/min per 100 g) and do not differ from rates determined with [6-14C]glucose (0.035 +- 0.005 vs. 0.026 +- 0.002). The minimal glucose masses and the mean transit times determined with both isotopes are also similar suggesting that plasma glucose levels and glucose turnover are maintained in fish fasted up to 40 days with no apparent increase in carbon recycling. Nonsteady-state isotope experiments suggest that these fish can alter rates of hepatic glucose production and peripheral uptake in response to hyper- and hypoglycemia

  1. Blood plasma levels of sex steroid hormones and vitellogenin in striped bass (morone saxatilis) exposed to 3,3{prime}, 4,4{prime}-Tetrachlorobiphenyl (TCB)

    Energy Technology Data Exchange (ETDEWEB)

    Monosson, E.; Fleming, W.J.; Sullivan, C.V. [North Carolina State Univ., Raleigh, NC (United States)] [and others

    1996-05-01

    Exposure to polychlorinated biphenyls (PCB) can impair reproductive processes in fish. Laboratory studies have demonstrated adverse effects in several different fish species. Evidence also exits for an association between exposure to PCBs and related compounds and impaired reproduction in wild fish. Although the mechanism of reproductive toxicity of PCBs is unclear, it appears that PCBs act of several different levels of the hypothalamus-pituitary-gonadal axis (HPG). Because of their structural similarity to 2,3,7,8-tetrachlorodibenzo-p-dioxin (dioxin), planar PCB congengers (e.g. 3,3`,4,4`-tetrachlorobiphenyl (TCB)) are among the most toxic PCBs. Both TCB and dioxon are reproductive toxicants in fish. TCB exposure (via intraperitoneal injections) impaired maturation in adult female white perch (Monroe americana) and reduced egg deposition in killifish (Fundulus heteroclitus). Larval or fry survival was also reduced following either maternal exposure to TCB for white perch or injections of TCB into fertilized eggs of rainbow trout. This study investigate the effects of exposure to TCB on reproductive processes in female striped bass. 12 refs., 2 tabs.

  2. Comparative study of 17 β-estradiol on endocrine disruption and biotransformation in fingerlings and juveniles of Japanese sea bass Lateolabrax japonicus.

    Science.gov (United States)

    Thilagam, Harikrishnan; Gopalakrishnan, Singaram; Bo, Jun; Wang, Ke-Jian

    2014-08-30

    Estrogenic contaminants in the aquatic environment are associated with endocrine disruption and feminization in fish. The effects of endocrine disrupting chemicals (EDCs) on fish have been well documented. However, very few studies have focused on 17 β-estradiol (E2) and its effects on endocrine system and biotransformation in a single prolonged exposure. This study investigated changes in the levels of serotonin (5-hydroxytryptamine) and acetyl choline esterase (AchE) in brain, cortisol in plasma and Ethoxyresorufin-O-deethylase (EROD) activity in gill of two different size groups (fingerlings and juveniles) of Japanese sea bass (Lateolabrax japonicus) upon exposure to two sub-lethal concentrations (200 and 2000 ng L(-1)) of E2 for 30 d. The results indicate that cortisol level and EROD activity significantly increased in both groups, whereas serotonin level increased in juveniles and decreased in fingerlings due to E2 exposure. The correlation analysis revealed that E2 significantly affected the endocrine and biotransformation systems in both age groups.

  3. 大口黑鲈微卫星DNA指纹图谱的构建和遗传结构分析%ESTABLISHMENT OF DNA FINGERPRINTING AND ANALYSIS ON GENETIC STRUCTURE OF LARGEMOUTH BASS WITH MICROSATELLITE

    Institute of Scientific and Technical Information of China (English)

    樊佳佳; 白俊杰; 李胜杰; 任坤; 叶星

    2012-01-01

    allozymes in recent years as the genetic markers of choice for many biological problems including parentage assessment, genomic mapping and establishment DNA fingerprinting. Largemouth bass (Micropterus sal-moides) was introduced from Taiwan to Guangdong province of China in 1983, because of delicious taste, fast growth, and wide temperature tolerance, it has been bred in many region of China as a main cultured fish species with annual production of 100,000 tons. The largemouth bass consists of two subspecies, the Florida largemouth bass (M. s. floridanus), which is native to peninsular Florida, and the northern largemouth bass (M. s. salmoides), whose native range extends throughout the central and eastern U.S. as well as northeastern Mexico and southeastern Canada. Research shows that largemouth bass cultured in china attribute to the northern largemouth bass (M .s. salmoides). In this paper, the microsatellite DNA fingerprinting of four largemouth bass populations was established, including cultured stock of M. salmoides in China (CH), M. s. floridanus was introduced in 2009 (FL-09), M. s. floridanus was introduced in 2010 (FL-10) and M. s. salmoides was introduced in 2010 (NT-10), and the genetic structure of them was analyzed by 43 microsatellite markers. The results showed that a total of 246 alleles were obtained from the four largemouth bass populations, and the alleles ranged from 2-13 in each locus. The mean value of number on alleles (A) and the mean value of expected heterozygosity (He) were calculated by popgene 3.2 software. The result was that the mean value of number on alleles {A) of CH, FL-09, FL-10 and NT-10 was 2.58, 3.74, 3.70 and 4.21, the mean value of expected heterozygosity (He) was 0.4549, 0.4896, 0.5010 and 0.6138, and the mean value of polymorphism information content (PIC) was 0.3786, 0.4443, 0.4566 and 0.5546, respectively. It indicated that the genetic diversity of cultured stock on largemouth bass in China was lower than that of introduced

  4. Biological Characteristics and Progress of Artificial Breeding Technique for Kelp Bass, Epinephelus moara%云纹石斑鱼生物学特性及人工繁育技术研究进展

    Institute of Scientific and Technical Information of China (English)

    宋振鑫; 陈超; 翟介明; 马文辉; 庞尊方

    2012-01-01

    Kelp bass Epinephelus moara, a fast growing and commercially valuable marine fish species, is considered as one of the most potential species for marine fish cage culture in the temperate seas along the coast of Northeast Asia. It has been investigated intensively for aquaculture during the past several decades in several countries. This article briefly summarized the biological characteristics of Kelp bass, further research priorities in this domain were also suggested. The progress on broodstoek reproduction, larval rearing, and the nursery of juveniles of artificial breeding in recently years were introduced. This paper also provides necessary referential information for the research on the mass seed production technique of Kelp bass in China.%云纹石斑鱼(Epinephelusmoara)具有耐受性强、生长速度快、质量优、价格高等特点,是温带海域养殖的理想品种。本文简要概括了云纹石斑鱼的生物学特性,以及近几年的人工繁育技术的研究现状,重点在亲鱼选择与强化培育、催产与受精卵孵化以及仔稚鱼培育方面取得的研究进展及就其发展前景进行分析,旨在为我国云纹石斑鱼的苗种规模化繁育技术研发提供参考。

  5. Evaluación de las técnicas de cepillado de bass y vertical en un grupo de pacientes en tratamiento de ortodoncia con técnica mbt

    OpenAIRE

    Yelitza Ivone Baracaldo Ortíz; Fredy Alexander Cabuya Cabuya; Rubén Darío Hurtado Rozo; Edwin Emil Ricaurte Vila; Diana Constanza Gamboa Martínez; Liliana Carolina Báez Quintero

    2012-01-01

    Objetivo: Evaluar la efectividad de las técnicas de Cepillado de Bass y Vertical en pacientes con aparatología ortodóntica fija tratados con la técnica MBT. Materiales y métodos: se realizó un estudio experimental “In vivo” aleatorizado doble ciego; la muestra fue de 100 personas distribuidas en dos grupos, a cada uno se le asignó una Técnica de Cepillado y se dieron instrucciones de higiene oral por medio de charlas personalizadas y por escrito. Se usó el índice de Sillnes y Löe para medir e...

  6. Isolation of TDA-producing Phaeobacter strains from sea bass larval rearing units and their probiotic effect against pathogenic Vibrio spp. in Artemia cultures

    DEFF Research Database (Denmark)

    Grotkjær, Torben; Bentzon-Tilia, Mikkel; D'Alvise, Paul;

    2016-01-01

    Fish-pathogenic Vibrio can cause large-scale crashes in marine larval rearing units and, since the use of antibiotics can result in bacterial antibiotic resistance, new strategies for disease prevention are needed. Roseobacter-clade bacteria from turbot larval rearing facilities can antagonize...... Vibrio anguillarum and reduce mortality in V. anguillarum-infected cod and turbot larvae. In this study, it was demonstrated that antagonistic Roseobacter-clade bacteria could be isolated from sea bass larval rearing units. In addition, it was shown that they not only antagonized V. anguillarum but also...

  7. Branchial expression and localization of the insulin-like growth factor 1 (IGF-1) receptor and changes in plasma IGF-1 and IGF-1 binding protein in striped bass during salinity acclimation

    DEFF Research Database (Denmark)

    Tipsmark, Christian Kølbæk; Madsen, Steffen; Borski, Russell

    2006-01-01

    acclimation. In the present study, primers for the IGF type 1 receptor from striped bass (Morone saxatilis) were designed for a real-time quantitative PCR assay. Two salinity transfer experiments were performed and the time-course of gill IGF-1R expression was examined in parallel with changes in plasma IGF-1...... concentration increased rapidly after FW transfer (6 hours and 1 day). Levels of a 37 kDa IGF binding protein (IGFBP-3 like), as measured by Western ligand blot, increased 2-fold in the plasma after transfer from FW to SW.  The cellular localization of the IGF-1R was investigated by immunohistochemistry using...

  8. DNA methylation of the gonadal aromatase (cyp19a promoter is involved in temperature-dependent sex ratio shifts in the European sea bass.

    Directory of Open Access Journals (Sweden)

    Laia Navarro-Martín

    2011-12-01

    Full Text Available Sex ratio shifts in response to temperature are common in fish and reptiles. However, the mechanism linking temperature during early development and sex ratios has remained elusive. We show in the European sea bass (sb, a fish in which temperature effects on sex ratios are maximal before the gonads form, that juvenile males have double the DNA methylation levels of females in the promoter of gonadal aromatase (cyp19a, the enzyme that converts androgens into estrogens. Exposure to high temperature increased the cyp19a promoter methylation levels of females, indicating that induced-masculinization involves DNA methylation-mediated control of aromatase gene expression, with an observed inverse relationship between methylation levels and expression. Although different CpGs within the sb cyp19a promoter exhibited different sensitivity to temperature, we show that the increased methylation of the sb cyp19a promoter, which occurs in the gonads but not in the brain, is not a generalized effect of temperature. Importantly, these effects were also observed in sexually undifferentiated fish and were not altered by estrogen treatment. Thus, methylation of the sb cyp19a promoter is the cause of the lower expression of cyp19a in temperature-masculinized fish. In vitro, induced methylation of the sb cyp19a promoter suppressed the ability of SF-1 and Foxl2 to stimulate transcription. Finally, a CpG differentially methylated by temperature and adjacent to a Sox transcription factor binding site is conserved across species. Thus, DNA methylation of the aromatase promoter may be an essential component of the long-sought-after mechanism connecting environmental temperature and sex ratios in vertebrate species with temperature-dependent sex determination.

  9. DNA Methylation of the Gonadal Aromatase (cyp19a) Promoter Is Involved in Temperature-Dependent Sex Ratio Shifts in the European Sea Bass

    Science.gov (United States)

    Navarro-Martín, Laia; Viñas, Jordi; Ribas, Laia; Díaz, Noelia; Gutiérrez, Arantxa; Di Croce, Luciano; Piferrer, Francesc

    2011-01-01

    Sex ratio shifts in response to temperature are common in fish and reptiles. However, the mechanism linking temperature during early development and sex ratios has remained elusive. We show in the European sea bass (sb), a fish in which temperature effects on sex ratios are maximal before the gonads form, that juvenile males have double the DNA methylation levels of females in the promoter of gonadal aromatase (cyp19a), the enzyme that converts androgens into estrogens. Exposure to high temperature increased the cyp19a promoter methylation levels of females, indicating that induced-masculinization involves DNA methylation-mediated control of aromatase gene expression, with an observed inverse relationship between methylation levels and expression. Although different CpGs within the sb cyp19a promoter exhibited different sensitivity to temperature, we show that the increased methylation of the sb cyp19a promoter, which occurs in the gonads but not in the brain, is not a generalized effect of temperature. Importantly, these effects were also observed in sexually undifferentiated fish and were not altered by estrogen treatment. Thus, methylation of the sb cyp19a promoter is the cause of the lower expression of cyp19a in temperature-masculinized fish. In vitro, induced methylation of the sb cyp19a promoter suppressed the ability of SF-1 and Foxl2 to stimulate transcription. Finally, a CpG differentially methylated by temperature and adjacent to a Sox transcription factor binding site is conserved across species. Thus, DNA methylation of the aromatase promoter may be an essential component of the long-sought-after mechanism connecting environmental temperature and sex ratios in vertebrate species with temperature-dependent sex determination. PMID:22242011

  10. A new Liopropoma sea bass (Serranidae, Epinephelinae, Liopropomini) from deep reefs off Curaçao, southern Caribbean, with comments on depth distributions of western Atlantic liopropomins

    Science.gov (United States)

    Baldwin, Carole C.; Robertson, D. Ross

    2014-01-01

    Collecting reef-fish specimens using a manned submersible diving to 300 m off Curaçao, southern Caribbean, is resulting in the discovery of numerous new fish species. The new Liopropoma sea bass described here differs from other western Atlantic members of the genus in having VIII, 13 dorsal-fin rays; a moderately indented dorsal-fin margin; a yellow-orange stripe along the entire upper lip; a series of approximately 13 white, chevron-shaped markings on the ventral portion of the trunk; and a reddish-black blotch on the tip of the lower caudal-fin lobe. The new species, with predominantly yellow body and fins, closely resembles the other two “golden basses” found together with it at Curaçao: L. aberransand L. olneyi. It also shares morphological features with the other western Atlantic liopropomin genus,Bathyanthias. Preliminary phylogenetic data suggest that western Atlantic liopropomins, includingBathyanthias, are monophyletic with respect to Indo-Pacific Liopropoma, and that Bathyanthias is nested within Liopropoma, indicating a need for further study of the generic limits of Liopropoma. The phylogenetic data also suggest that western Atlantic liopropomins comprise three monophyletic clades that have overlapping depth distributions but different depth maxima (3–135 m, 30–150 m, 133–411 m). The new species has the deepest depth range (182–241 m) of any known western Atlantic Liopropomaspecies. Both allopatric and depth-mediated ecological speciation may have contributed to the evolution of western Atlantic Liopropomini.

  11. Chemical contaminants in water and sediment near fish nesting sites in the Potomac River basin: determining potential exposures to smallmouth bass (Micropterus dolomieu)

    Science.gov (United States)

    Kolpin, Dana W.; Blazer, Vicki; Gray, James L.; Focazio, Michael J.; Young, John A.; Alvarez, David A.; Iwanowicz, Luke R.; Foreman, William T.; Furlong, Edward T.; Speiran, Gary K.; Zaugg, Steven D.; Hubbard, Laura E.; Meyer, Michael T.; Sandstrom, Mark W.; Barber, Larry B.

    2013-01-01

    The Potomac River basin is an area where a high prevalence of abnormalities such as testicular oocytes (TO), skin lesions, and mortality has been observed in smallmouth bass (SMB, Micropterus dolomieu). Previous research documented a variety of chemicals in regional streams, implicating chemical exposure as one plausible explanation for these biological effects. Six stream sites in the Potomac basin (and one out-of-basin reference site) were sampled to provide an assessment of chemicals in these streams. Potential early life-stage exposure to chemicals detected was assessed by collecting samples in and around SMB nesting areas. Target chemicals included those known to be associated with important agricultural and municipal wastewater sources in the Potomac basin. The prevalence and severity of TO in SMB were also measured to determine potential relations between chemistry and biological effects. A total of 39 chemicals were detected at least once in the discrete-water samples, with atrazine, caffeine, deethylatrazine, simazine, and iso-chlorotetracycline being most frequently detected. Of the most frequently detected chemicals, only caffeine was detected in water from the reference site. No biogenic hormones/sterols were detected in the discrete-water samples. In contrast, 100 chemicals (including six biogenic hormones/sterols) were found in a least one passive-water sample, with 25 being detected at all such samples. In addition, 46 chemicals (including seven biogenic hormones/sterols) were found in the bed-sediment samples, with caffeine, cholesterol, indole, para-cresol, and sitosterol detected in all such samples. The number of herbicides detected in discrete-water samples per site had a significant positive relation to TOrank (a nonparametric indicator of TO), with significant positive relations between TOrank and atrazine concentrations in discrete-water samples and to total hormone/sterol concentration in bed-sediment samples. Such significant correlations

  12. Black bass (Micropterus salmoides, Lacepède 1802 fingerlings performance and survival, submitted to the alimentary conditioning, using different proteics pâtés / Desempenho e sobrevivência de alevinos de black bass (Micropterus salmoides, Lacepède 1802, submetidos ao condicionamento alimentar, utilizando diferentes patês protéicos

    Directory of Open Access Journals (Sweden)

    Arcangelo Augusto Signor

    2008-08-01

    Full Text Available The aim of this study was to evaluate the use of artificial rations with different proteics pâtés incorporated in the black bass (Micropterus salmoides fingerlings diet. 320 fingerlings, with initial average weight of 0,57±0,1g and lenght of 3,61±0,21cm, respectively were used. The fish were distributed in an entirely casualized delineation in 32 polypropylene boxes of 310 liters, with 4 treatments and 8 repetitions. One reference ration (RR with 38% PB as diet base was used, being added to each treatment proteics pâtés (PP, composites of bovine heart (CB, eviscerate sardine (SE, Chilean fish flour (FP and tilapia fillet (FT. During the alimentary training good acceptance of the artificial diets was observed, with better performance results for the treatments with FP and FT.O objetivo deste trabalho foi avaliar a utilização de rações artificiais com diferentes patês protéicos incorporados na dieta de alevinos de black bass (Micropterus salmoides. Foram utilizados 320 alevinos, com peso e comprimento inicial médio de 0,57±0,1g e 3,61±0,21cm, respectivamente. Os peixes foram distribuídos em um delineamento inteiramente casualizado em 32 caixas de polipropileno de 310 litros, com 4 tratamentos e 8 repetições. Utilizou-se uma ração referência (RR com 38% PB como base da dieta, sendo adicionada patês protéicos (PP referente a cada tratamento, compostos por coração bovino (CB, sardinha eviscerada (SE, farinha de peixe chilena (FP e filé de tilápia (FT. Durante o treinamento alimentar foi observado boa aceitação as dietas artificiais, com melhores resultados de desempenho para os tratamentos com FP e FT.

  13. Connectivity across the Caribbean Sea: DNA barcoding and morphology unite an enigmatic fish larva from the Florida straits with a new species of sea bass from deep reefs off Curaçao.

    Science.gov (United States)

    Baldwin, Carole C; Johnson, G David

    2014-01-01

    Integrative taxonomy, in which multiple disciplines are combined to address questions related to biological species diversity, is a valuable tool for identifying pelagic marine fish larvae and recognizing the existence of new fish species. Here we combine data from DNA barcoding, comparative morphology, and analysis of color patterns to identify an unusual fish larva from the Florida Straits and demonstrate that it is the pelagic larval phase of a previously undescribed species of Liopropoma sea bass from deep reefs off Curaçao, southern Caribbean. The larva is unique among larvae of the teleost family Serranidae, Tribe Liopropomini, in having seven elongate dorsal-fin spines. Adults of the new species are similar to the golden bass, Liopropoma aberrans, with which they have been confused, but they are distinct genetically and morphologically. The new species differs from all other western Atlantic liopropomins in having IX, 11 dorsal-fin rays and in having a unique color pattern-most notably the predominance of yellow pigment on the dorsal portion of the trunk, a pale to white body ventrally, and yellow spots scattered across both the dorsal and ventral portions of the trunk. Exploration of deep reefs to 300 m using a manned submersible off Curaçao is resulting in the discovery of numerous new fish species, improving our genetic databases, and greatly enhancing our understanding of deep-reef fish diversity in the southern Caribbean. Oh the mother and child reunion is only a moment away. Paul Simon. PMID:24825118

  14. Connectivity across the Caribbean Sea: DNA barcoding and morphology unite an enigmatic fish larva from the Florida straits with a new species of sea bass from deep reefs off Curacao.

    Directory of Open Access Journals (Sweden)

    Carole C Baldwin

    Full Text Available Integrative taxonomy, in which multiple disciplines are combined to address questions related to biological species diversity, is a valuable tool for identifying pelagic marine fish larvae and recognizing the existence of new fish species. Here we combine data from DNA barcoding, comparative morphology, and analysis of color patterns to identify an unusual fish larva from the Florida Straits and demonstrate that it is the pelagic larval phase of a previously undescribed species of Liopropoma sea bass from deep reefs off Curaçao, southern Caribbean. The larva is unique among larvae of the teleost family Serranidae, Tribe Liopropomini, in having seven elongate dorsal-fin spines. Adults of the new species are similar to the golden bass, Liopropoma aberrans, with which they have been confused, but they are distinct genetically and morphologically. The new species differs from all other western Atlantic liopropomins in having IX, 11 dorsal-fin rays and in having a unique color pattern-most notably the predominance of yellow pigment on the dorsal portion of the trunk, a pale to white body ventrally, and yellow spots scattered across both the dorsal and ventral portions of the trunk. Exploration of deep reefs to 300 m using a manned submersible off Curaçao is resulting in the discovery of numerous new fish species, improving our genetic databases, and greatly enhancing our understanding of deep-reef fish diversity in the southern Caribbean. Oh the mother and child reunion is only a moment away. Paul Simon.

  15. 基于BASS模型的危机信息扩散模式%Crisis Information Diffusion Model Based on BASS Model

    Institute of Scientific and Technical Information of China (English)

    魏玖长; 周磊; 赵定涛

    2011-01-01

    Effective crisis information dissemination is critical in crisis management.In order to study the diffusion characteristics of crisis information under different modes of transmission,this paper,based on BASS Model,constructs the diffusion model of true information and rumor information after the public crisis,simulates the diffusion rules and state differences of two types of information under the condition of different parameters through the Matlab software.The result shows that the growth of two types of information diffusion curve is affected by channel coefficients;that it has positive relationship between the final scale and information accuracy in informal channels.To improve the effectiveness of crisis information dissemination,crisis management departments could expand the coverage of formal channels,improve the accuracy and authority of information issuance,enhance the public emergency education and change the rumor management attitude.%有效的危机信息传播在危机管理中至关重要。为了研究危机信息在不同传播方式下的扩散特征,本文基于BA SS模型,构建了公共危机事件后正确信息与谣言信息的扩散模型,并通过M atlab软件模拟了不同参数条件下两类信息的扩散规律及扩散状态差异。分析结果表明,两类信息扩散曲线的增长幅度受到渠道系数的影响,最终的扩散规模与非正式渠道信息准确度存在正相关的关系。要提高危机信息传播的有效性,危机管理部门可以从扩大正式渠道的覆盖范围、提高信息发布的准确性与权威性、加强公众的应急素质教育以及转变谣言管理态度等方面入手。

  16. Impacts of Dust on Tropical Volcanic Soil Formation: Insights from Strontium and Uranium-Series Isotopes in Soils from Basse-Terre Island, French Guadeloupe

    Science.gov (United States)

    Pereyra, Y.; Ma, L.; Sak, P. B.; Gaillardet, J.; Buss, H. L.; Brantley, S. L.

    2015-12-01

    Dust inputs play an important role in soil formation, especially for thick soils developed on tropical volcanic islands. In these regions, soils are highly depleted due to intensive chemical weathering, and mineral nutrients from dusts have been known to be important in sustaining soil fertility and productivity. Tropical volcanic soils are an ideal system to study the impacts of dust inputs on the ecosystem. Sr and U-series isotopes are excellent tracers to identify sources of materials in an open system if the end-members have distinctive isotope signatures. These two isotope systems are particularly useful to trace the origin of atmospheric inputs into soils and to determine rates and timescales of soil formation. This study analyzes major elemental concentrations, Sr and U-series isotope ratios in highly depleted soils in the tropical volcanic island of Basse-Terre in French Guadeloupe to determine atmospheric input sources and identify key soil formation processes. We focus on three soil profiles (8 to 12 m thick) from the Bras-David, Moustique Petit-Bourg, and Deshaies watersheds; and on the adjacent rivers to these sites. Results have shown a significant depletion of U, Sr, and major elements in the deep profile (12 to 4 m) attributed to rapid chemical weathering. The top soil profiles (4 m to the surface) all show addition of elements such as Ca, Mg, U, and Sr due to atmospheric dust. More importantly, the topsoil profiles have distinct Sr and U-series isotope compositions from the deep soils. Sr and U-series isotope ratios of the top soils and sequential extraction fractions confirm that the sources of the dust are from the Saharan dessert, through long distance transport from Africa to the Caribbean region across the Atlantic Ocean. During the transport, some dust isotope signatures may also have been modified by local volcanic ashes and marine aerosols. Our study highlights that dusts and marine aerosols play important roles in element cycles and

  17. Effet des additifs sur la structure cristalline du polyéthylène à basse densité (PEBD irradiés par des rayonnements UV

    Directory of Open Access Journals (Sweden)

    Cheloufi T

    2014-04-01

    Full Text Available L’effet des rayons U V sur les propriétés physico-chimiques et mécaniques du polyéthylène à basse densité (PEBD et pigmenté avec des additifs dans différentes formulations ont été étudiées. Les échantillons ont été exposés au rayonnement U V émis par une lampe à xénon d’une puissance de 6000 W à une température de 46 °C pour différents temps. Un échantillon de référence a été pris pour la comparaison. A l’aide de la spectroscopie infrarouge FTIR et l’essai de traction, on a pu suivre les changements structuraux physico-chimiques et mécaniques. L’évolution des paramètres mécaniques (module d’élasticité, contrainte et allongement à la rupture ainsi que l’évolution des groupements carbonylés sous l’effet des radiations UV sont discutées. L’allongement à la rupture diminue avec le temps d’exposition pour la plupart des formulations étudiées, sauf la formulation F4 (anti-oxydant/stabilisant dont il y à une augmentation avec le temps. Par les RX, on a pu calculer le taux de cristallinité qui ont donné des valeurs élevées par rapport à d’autres ce qui est du probablement aux additifs ajoutés (chimassorb 81. Les résultats obtenus montrent que les additifs (stabilisants lumière et anti-oxydants avec des concentrations appropriées donnent des améliorations fiables concernant le comportement mécanique et les propriétés physico-chimiques des échantillons du PEBD exposés au rayonnement UV.

  18. Nutrient budgets, marsh inundation under sea-level rise scenarios, and sediment chronologies for the Bass Harbor Marsh estuary at Acadia National Park

    Science.gov (United States)

    Huntington, Thomas G.; Culbertson, Charles W.; Fuller, Christopher C.; Glibert, Patricia; Sturtevant, Luke

    2014-01-01

    Eutrophication in the Bass Harbor Marsh estuary on Mount Desert Island, Maine, is an ongoing problem manifested by recurring annual blooms of green macroalgae species, principally Enteromorpha prolifera and Enteromorpha flexuosa, blooms that appear in the spring and summer. These blooms are unsightly and impair the otherwise natural beauty of this estuarine ecosystem. The macroalgae also threaten the integrity of the estuary and its inherent functions. The U.S. Geological Survey and Acadia National Park have collaborated for several years to better understand the factors related to this eutrophication problem with support from the U.S. Geological Survey and National Park Service Water Quality Assessment and Monitoring Program. The current study involved the collection of hydrologic and water-quality data necessary to investigate the relative contribution of nutrients from oceanic and terrestrial sources during summer 2011 and summer 2012. This report provides data on nutrient budgets for this estuary, sedimentation chronologies for the estuary and fringing marsh, and estuary bathymetry. The report also includes data, based on aerial photographs, on historical changes from 1944 to 2010 in estuary surface area and data, based on surface-elevation details, on changes in marsh area that may accompany sea-level rise. The LOADEST regression model was used to calculate nutrient loads into and out of the estuary during summer 2011 and summer 2012. During these summers, tidal inputs of ammonium to the estuary were more than seven times greater than the combined inputs in watershed runoff and precipitation. In 2011 tidal inputs of nitrate were about four times greater than watershed plus precipitation inputs, and in 2012 tidal inputs were only slightly larger than watershed plus precipitation inputs. In 2011, tidal inputs of total organic nitrogen were larger than watershed input by a factor of 1.6. By contrast, in 2012 inputs of total organic nitrogen in watershed runoff

  19. Recherche de pulsars, transitoires et exoplanètes à basses fréquences avec le radiotélescope UTR-2: méthodes et premiers résultats

    OpenAIRE

    Vasylieva, Iaroslavna

    2015-01-01

    La recherche et l’étude de sources radio transitoires, en particulier à basses fréquences, est un des projets clés de la nouvelle génération de radiotélescopes comme SKA et ses précurseurs, parmi lesquels LOFAR, NenuFAR et GURT. Ces sources incluent les étoiles à neutrons - produisant un signal périodique, détectées comme pulsars -, d’autres objets très magnétisés compacts ou des événements très dynamiques ou explosifs - produisant des transitoires non périodiques -, et des exoplanètes - dont...

  20. Modélisation et validation expérimentale des complexes insonorisants pour la prévision vibroacoustique numérique basse et moyenne fréquences des automobiles

    OpenAIRE

    Fernandez, Charles

    2008-01-01

    Dans cette recherche, on construit un modèle simplifié en basses et moyennes fréquences de complexes insonorisants (habillages) de l'industrie automobile à partir d'un élément élastoacoustique stochastique. Le modèle simplifié moyen est issu d'une extension de la théorie des structures floues et dépend de trois paramètres physiques : densité modale, amortissement et masse participante. Le modèle simplifié stochastique qui prend en compte les incertitudes de modèle et de données est construit ...

  1. Hacer globalización desde Ecuador. El tercer espacio como utopía para el ecuatoriano en Timarán y Cuabú de Nelson Estupiñán Bass

    Directory of Open Access Journals (Sweden)

    Wilfried Mvondo

    2016-07-01

    Full Text Available Este ensayo estudia cómo Nelson Estupiñán Bass da cuenta de la dinámica de la creación del tercer espacio en Ecuador con relación a la utopía de progreso socioeconómico que esta arrastra. Contempla la articulación entre “tercer espacio” y “utopía”. Este trabajo, que se apoya en el enfoque sociocrítico, analiza el espacio en Timarán y Cuabú y las inscripciones ideológicas que en ello afloran. Culmina en los siguientes resultados: 1 el tercer espacio es un lugar de intereses y resistencia para el ecuatoriano;y 2 este espacio suscita cierta utopía de desarrollo en el ecuatoriano. This paper studies how Nelson Estupiñán Bass realizes the dynamics in the creation of the third space in Ecuador relative to the utopia of socio-economic progress dragged by the latter. The study focuses on the articulation of the “third space” and the “utopia”. As this work is based on the sociocritical approach, it analyses the space from Timarán y Cuabú and the ideologies that it outcrops. The study deals with the following findings: 1 the third space is a space of interests and resistance for the Ecuadorian and 2 that space create a utopia of development in the Ecuadorian.

  2. Leptin stimulates hepatic growth hormone receptor and insulin-like growth factor gene expression in a teleost fish, the hybrid striped bass.

    Science.gov (United States)

    Won, Eugene T; Douros, Jonathan D; Hurt, David A; Borski, Russell J

    2016-04-01

    Leptin is an anorexigenic peptide hormone that circulates as an indicator of adiposity in mammals, and functions to maintain energy homeostasis by balancing feeding and energy expenditure. In fish, leptin tends to be predominantly expressed in the liver, another important energy storing tissue, rather than in fat depots as it is in mammals. The liver also produces the majority of circulating insulin-like growth factors (IGFs), which comprise the mitogenic component of the growth hormone (GH)-IGF endocrine growth axis. Based on similar regulatory patterns of leptin and IGFs that we have documented in previous studies on hybrid striped bass (HSB: Morone saxatilis×Morone chrysops), and considering the co-localization of these peptides in the liver, we hypothesized that leptin might regulate the endocrine growth axis in a manner that helps coordinate somatic growth with energy availability. Using a HSB hepatocyte culture system to simulate autocrine or paracrine exposure that might occur within the liver, this study examines the potential for leptin to modulate metabolism and growth through regulation of IGF gene expression directly, or indirectly through the regulation of GH receptors (GHR), which mediate GH-induced IGF expression. First, we verified that GH (50nM) has a classical stimulatory effect on IGF-1 and additionally show it stimulates IGF-2 transcription in hepatocytes. Leptin (5 and/or 50nM) directly stimulated in vitro GHR2 gene expression within 8h of exposure, and both GHR1 and GHR2 as well as IGF-1 and IGF-2 gene expression after 24h. Cells were then co-incubated with submaximal concentrations of leptin and GH (25nM each) to test if they had a synergistic effect on IGF gene expression, possibly through increased GH sensitivity following GHR upregulation by leptin. In combination, however, the treatments only had an additive effect on stimulating IGF-1 mRNA despite their capacity to increase GHR mRNA abundance. This suggests that leptin's stimulatory

  3. 基于Bass模型的新产品商业化最优时机%The Optimal Commercialization Timing of New Products Based on Bass Model

    Institute of Scientific and Technical Information of China (English)

    燕夏敏; 林军; 崔文田; 钱艳俊

    2011-01-01

    The optimal commercialization timing of new products has been given much attention by firms in Product Life Cycle(PLC).The previous research was almost based on real option,failing to express the S-diffusion feature of product demand in Product Life Cycle,and resulting in the differences between the actual situation and the theoretical analysis.As firms expect to cost minimization and profit maximization,this paper incorporates bass model as widely used in the diffusion field to the theory of real option,describing changes of demand throughout the Product Life Cycle,and establishing the model of optimal commercialization timing of new products.The result can help firms to make decisions on commercializing products.The research results show that the optimal commercialization timing of new products is robust,when price impact factor(the effect of price on market diffusion speed) is small enough;and the effect of price impact factor on the optimal commercialization timing is also related to the pricing policies.%在产品整个生命周期过程中,何时进行商业化是企业较为关注的一个问题。现有研究大多基于实物期权理论而未能考虑产品需求在生命周期过程中呈现的S型曲线扩散特征,因此分析结果与实际相差较大。本文基于企业期望成本最小化和利润最大化的本质属性,将广泛应用于新产品扩散领域的B ass模型引入到实物期权理论中,用其来刻画新产品在整个生命周期过程中需求的变化,从而建立新产品商业化最优时机问题的研究模型。结论能有效帮助企业制定新产品的商业化策略。研究结果发现当价格影响因子(价格对市场扩散速度的影响)足够小时,依据该模型确定的新产品商业化最优时机策略具有鲁棒性,并且价格影响因子对新产品商业化最优时机策略的影响与新产品所采用的价格策略有关。

  4. Impact of entrainment and impingement on fish populations in the Hudson River Estuary. Volume II. Impingement impact analyses, evaluations of alternative screening devices, and critiques of utility testimony relating to density-dependent growth, the age-composition of the striped bass spawning stock, and the LMS real-time life cycle model

    Energy Technology Data Exchange (ETDEWEB)

    Barnthouse, L. W.; Van Winkle, W.; Golumbek, J.; Cada, G. F.; Goodyear, C. P.; Christensen, S. W.; Cannon, J. B.; Lee, D. W.

    1982-04-01

    This volume includes a series of four exhibits relating to impacts of impingement on fish populations, together with a collection of critical evaluations of testimony prepared for the utilities by their consultants. The first exhibit is a quantitative evaluation of four sources of bias (collection efficiency, reimpingement, impingement on inoperative screens, and impingement survival) affecting estimates of the number of fish killed at Hudson River power plants. The two following exhibits contain, respectively, a detailed assessment of the impact of impingement on the Hudson River white perch population and estimates of conditional impingement mortality rates for seven Hudson River fish populations. The fourth exhibit is an evaluation of the engineering feasibility and potential biological effectiveness of several types of modified intake structures proposed as alternatives to cooling towers for reducing impingement impacts. The remainder of Volume II consists of critical evaluations of the utilities' empirical evidence for the existence of density-dependent growth in young-of-the-year striped bass and white perch, of their estimate of the age-composition of the striped bass spawning stock in the Hudson River, and of their use of the Lawler, Matusky, and Skelly (LMS) Real-Time Life Cycle Model to estimate the impact of entrainment and impingement on the Hudson River striped bass population.

  5. Impact of entrainment and impingement on fish populations in the Hudson River Estuary. Volume II. Impingement impact analyses, evaluations of alternative screening devices, and critiques of utility testimony relating to density-dependent growth, the age-composition of the striped bass spawning stock, and the LMS real-time life cycle model

    International Nuclear Information System (INIS)

    This volume includes a series of four exhibits relating to impacts of impingement on fish populations, together with a collection of critical evaluations of testimony prepared for the utilities by their consultants. The first exhibit is a quantitative evaluation of four sources of bias (collection efficiency, reimpingement, impingement on inoperative screens, and impingement survival) affecting estimates of the number of fish killed at Hudson River power plants. The two following exhibits contain, respectively, a detailed assessment of the impact of impingement on the Hudson River white perch population and estimates of conditional impingement mortality rates for seven Hudson River fish populations. The fourth exhibit is an evaluation of the engineering feasibility and potential biological effectiveness of several types of modified intake structures proposed as alternatives to cooling towers for reducing impingement impacts. The remainder of Volume II consists of critical evaluations of the utilities' empirical evidence for the existence of density-dependent growth in young-of-the-year striped bass and white perch, of their estimate of the age-composition of the striped bass spawning stock in the Hudson River, and of their use of the Lawler, Matusky, and Skelly (LMS) Real-Time Life Cycle Model to estimate the impact of entrainment and impingement on the Hudson River striped bass population

  6. Isolation and Identification of Nocardia, A Pathogen of Nocardiosis in Largemouth Bass, Micropterus salmoides%加州鲈诺卡菌病病原的分离与鉴定

    Institute of Scientific and Technical Information of China (English)

    蒋依依; 李言伟; 周素明; 李安兴

    2012-01-01

    Nocardiosis is a commercially important disease in cultured largemouth bass in China, causing substantial economic losses. However, the pathogen causing largemouth bass nocardiosis has not yet been isolated in China. In this study, a strain of Nocardia (designed as NH090627 ) was isolated from diseased largemouth bass reared in Nanhai, Guangdong. The bacteria appeared Gram-positive, bead-like or long, slender, filamentous rods in shapes. The traditional physiological and biochemical methods showed that most of the phenotype of strain NH090627 were similar to Nocardia. Phylogeny analysis with 16S rDNA showed that the strain NH090627 was branched into the same cluster as that of other species of Nocardia , and had a higher identity with Nocardia seriolae JCM 3360T (99. 9% ). Based on morphological, physiological, biochemical properties and phylogenetic analysis, the filamentous rod bacterial was identified as Nocardia seriolea. Antimicrobial susceptibility assay showed that this strain was susceptible to chloramphenicol, streptomycin, kanamycin and tetracycline, but high resistant to sulphamethoxazole and ampicillin.%加州鲈是我国南方的重要经济鱼类养殖品种,但近年病害问题十分突出.诺卡菌已成为危害加州鲈健康养殖的重要病原,目前对该病原菌的分离鉴定及种属类型的研究尚未见报道.国内首次从患病加州鲈体内分离得到诺卡菌,并最终确定了其病原种类是鰤鱼诺卡菌Nocardia seriolea.从患病加州鲈内分离到菌株NH090627,镜检发现该菌为革兰氏阳性的分支状杆菌.生理生化鉴定结果表明分离菌株与诺卡菌属Nocardia细菌的基本特征相符.回归感染试验证实菌株NH090627即为引起此次加州鲈结节病的病原菌.对该菌株的16SrDNA进行了扩增和序列分析发现,该菌株与诺卡菌属细菌的亲缘关系较近,且与鰤鱼诺氏菌N.seriolea JCM3360的16S rDNA序列同源性最高,达到99.9%.综合上述试验结果,我们

  7. High Throughput Approach Applied to VOC Oxidation at Low Temperature Approche haut débit appliquée à l’oxydation basse température des COV

    Directory of Open Access Journals (Sweden)

    Jolly J.

    2013-03-01

    Full Text Available A High Throughput (HT approach is used to prepare and characterize the catalytic activity of materials for the destruction of Volatile Organic Compounds (VOC at low temperature. The interest of the Designs of Experiments (DoE in the primary screening is demonstrated through modeling catalysts composition according to the light-off temperature detected by infrared thermography. The combination of the mass spectrometry and the infrared thermography appears very useful to confirm and increase information about catalytic properties of the catalysts extracted to the primary screening stage. Finally, in situ Raman spectroscopy with infrared thermography allows to better understand the origin of the catalytic properties in the case ofAu/CeO2 catalysts. A strong interaction between gold and Ce-O bonds leading to an improvement of the cerium oxide reducibility could explain its high reactivity for 2-propanol oxidation at lower temperature. Une approche haut debit (HD est utilisée de la préparation jusqu’à la caractérisation de l’activité catalytique des matériaux pour l’élimination des Composés Organiques Volatils (COV à basse température. L’intérêt des plans d’expériences (DoE, en anglais Designs of Experiments dans le screening primaire est démontré grâce à l’identification d’une composition optimale de catalyseur prédite à l’aide des différentes températures d’ignition des catalyseurs obtenues par thermographie infrarouge. La combinaison in situ de la spectrométrie de masse et de la thermographie infrarouge s’est avérée d’une grande utilité pour confirmer et augmenter le niveau d’information concernant les propriétés catalytiques des catalyseurs provenant du screening primaire. Enfin, la spectroscopie Raman in situ couplée à la thermographie infrarouge permet de mieux comprendre l’origine des propriétés catalytiques dans le cas du catalyseur Au/CeO2. Une forte interaction entre les particules d

  8. 超高压对海鲈鱼鱼肉的蒸煮损失及蛋白质降解程度的影响%Effects of High Pressure Treatment on Cooking Loss and Protein Denaturation of Sea Bass

    Institute of Scientific and Technical Information of China (English)

    郑捷; 尚校兰; 刘安军

    2013-01-01

    Relatively high pressure processing could result in a decrease in cooking loss of sea bass,but the cooking loss value would increase with the extension of storage time. Pressure of 400,MPa was conducive for the maintainance of three-dimensional gel grid structure,and the cooking loss value showed no significant difference compared with the control. In addition,high pressure treatment could result in denaturation of sarcoplasmic protein and myofibrillar protein,large mole-cules would become small molecules,and the degradation degree increased with the extension of storage time.%  适当的超高压处理有利于降低海鲈鱼鱼肉的蒸煮损失。但是随着贮藏时间的延长,蒸煮损失升高,而400,MPa 的压力处理海鲈鱼的蒸煮损失表现为不显著水平。另外,超高压处理会使肌浆蛋白和肌原纤维蛋白变性,且随着贮藏时间的延长,大分子变成小分子,降解程度加大。

  9. 基于Bass模型的我国大学技术创新扩散实证分析%An Empirical Analysis of Technology Innovation Diffusion in Chinese Universities Based on Bass Model

    Institute of Scientific and Technical Information of China (English)

    黄海洋; 王晴; 陈继祥

    2013-01-01

    Based on the analysis of the Bass model which is the most representative of innovation diffusion models,and the characteristics of technology innovation diffusion of universities,this paper has established a improved model for the technology innovation diffusion in universities taking repeated purchase as an variable.Based on the regression analysis of the income of technology transfer in Chinese universities,an equation for technology innovation diffusion in universities is obtained,and a forward-looking predictive analysis is given.%在研究分析创新扩散模型中最具代表性的Bass模型的基础上,结合大学技术创新扩散的特点,把重复购买作为变量建立了适用于大学技术创新扩散的改进模型.以我国大学技术成果转让收入作为样本数据进行回归分析,得出大学技术创新扩散的方程式,并进行前瞻性的预测分析.

  10. The Research on User Diffusion of China's Mobile Internet Based on the Bass Model%基于Bass模型的中国移动互联网用户扩散研究

    Institute of Scientific and Technical Information of China (English)

    程静薇

    2013-01-01

    Mobile Internet has developed rapidly in China in recent years. Relative researches have sug-gested that the process of user diffusion of mobile internet is closed to a s-shaped curve. This essay made an empirical analysis on the process of user diffusion of China's user of mobile internet by the method of the Bass model. It turned out that the imitation coefficient is higher and the innovation coefficient is low-er. The essay has also forecasted that the number of active users of China's mobile Internet will reach to 756 million by 2016 .%移动互联网近年来在我国迅速发展,已有研究表明移动互联网的用户扩散过程呈S型生长曲线。本文使用Bass模型对中国移动互联网用户扩散过程进行了实证分析,结果表明:移动互联网的用户扩散模仿系数较大、创新系数较小,并预测至2016年,中国移动互联网活跃用户数将达到7.56亿。

  11. Relationship of weed shiner and young-of-year bluegill and largemouth bass abundance to submersed aquatic vegetation in Navigation Pools 4, 8, and 13 of the Upper Mississippi River, 1998-2012

    Science.gov (United States)

    DeLain, Steven A.; Popp, Walter A.

    2014-01-01

    Aquatic vegetation provides food resources and shelter for many species of fish. This study found a significant relationship between increases in submersed aquatic vegetation (SAV) in four study reaches of the Upper Mississippi River (UMR) and increases in catch-per-unit-effort (CPUE) of weed shiners (Notropis texanus) and age-0 bluegills (Lepomis macrochirus) and largemouth bass (Micropterus salmoides) when all of the study reaches were treated collectively using Long Term Resource Monitoring Program (LTRMP) vegetation and fish data for 1998–2012. The selected fishes were more abundant in study reaches with higher SAV frequencies (Pool 8 and Lower Pool 4) and less abundant in reaches with lower SAV frequencies (Pool 13 and Upper Pool 4). When each study reach was examined independently, the relationship between SAV frequency and CPUE of the three species was not significant in most cases, the primary exception being weed shiners in Lower Pool 4. Results of this study indicate that the prevalence of SAV does affect relative abundance of these vegetation-associated fish species. However, the poor annual relationship between SAV frequency and age-0 relative abundance in individual study reaches indicates that several other factors also govern age-0 abundance. The data indicate that there may be a SAV frequency threshold in backwaters above which there is not a strong relationship with abundance of these fish species. This is indicated by the high annual CPUE variability of the three selected fishes in backwaters of Pool 8 and Lower Pool 4 when SAV exceeded certain frequencies.

  12. Biology of black bass Micropterus salmoides (Lacepède, 1802 fifty years after the introduction in a small drainage of the Upper Paraná River basin, Brazil

    Directory of Open Access Journals (Sweden)

    DIEGO AZEVEDO ZOCCAL GARCIA

    2014-10-01

    Full Text Available Garcia DAZ, Costa ADA, Leme GLA, Orsi ML. 2014. Biology of black bass Micropterus salmoides (Lacepède, 1802 fifty years after the introduction in a small drainage of the Upper Paraná River basin, Brazil. Biodiversitas 15: 180-185. The dispersion of organisms by human actions has been the major source of changes in the natural distribution of species, making the introduction of non-native species a threat to biological diversity. Micropterus salmoides is a fish originating from North America, which was introduced in a lagoon in the Ecological Park of Fazenda Monte Alegre in southern Brazil over 50 years ago. The reproductive activity, weight-length relationship and relative condition factor were analyzed to evaluate the health parameters of the species. The result allows us to classify the reproductive activity of this population as moderate. It was found that the health condition patterns are identical to those theoretically expected. The occurrence of individuals downstream of the lagoon shows that the population has been presenting dispersion conditions to new environments, posing a threat to local biodiversity. Management measures, such as isolation or eradication of the population, are required to control the species within the studied site, and prevent its dispersion into natural watercourses.

  13. Analysis of Innovation Diffusion with Multiple Generations Based on the Norton-Bass Model%基于Norton-Bass模型的多代创新产品扩散研究

    Institute of Scientific and Technical Information of China (English)

    胡知能; 邓欢; 张弛; 徐玖平

    2012-01-01

    本文基于多代创新产品的扩散特点,首先建立了三代产品扩散的基本模型,然后,结合实际的产品营销活动,将免费赠送商品、产品定价策略和商品的重复购买等因素分别引入该基本模型,建立了相应的扩展模型,在此基础上,以免费商品赠送率为重点考察对象,分别对基本模型和扩展模型进行了分析.研究发现重复购买者的出现会导致产品的销售峰值上升,总利润增加和销售周期的延长,且会促使生产厂商采取更大比例的赠品率来促进总利润的增加.%The research of product diffusion models began in the early 20th century and has gradually become active since 1960s. The Bass ( 1969) model is one of the most well-known and widely used models used to understand the demand for the first purchase. Bass's original logistic formulation has received a number of modifications and extensions. Multi-generation innovation diffusion can be seen as a special case for the diffusion of competitive products. In the product diffusion area the most famous model is Norton and Bass's (1987) model. In the process of diffusing multi-generation products, the interaction between each generation product reflects customers' preference to different products in their purchasing behavior. When there is only one generation product diffusion in the market, its diffusion process is similar to Bass model. That is the case of normal diffusion of a single product. But when there are new generation products, the market structure will be changed accordingly. The new generation product has upgraded and improved features and can attract new customers. Meanwhile, some consumers who prepare to buy the previous generation product will be attracted by the features of the new generation product, and instead purchase the new generation product. The purchasing behavior of these consumers is not necessarily the same as their original purchase behavior. So it is necessary to explore such

  14. Heavy metal ion inhibition studies of human, sheep and fish α-carbonic anhydrases.

    Science.gov (United States)

    Demirdağ, Ramazan; Yerlikaya, Emrah; Şentürk, Murat; Küfrevioğlu, Ö İrfan; Supuran, Claudiu T

    2013-04-01

    Carbonic anhydrases (CAs, EC 4.2.1.1) were purified from sheep kidney (sCA IV), from the liver of the teleost fish Dicentrarchus labrax (dCA) and from human erythrocytes (hCA I and hCA II). The purification procedure consisted of a single step affinity chromatography on Sepharose 4B-tyrosine-sulfanilamide. The kinetic parameters of these enzymes were determined for their esterase activity with 4-nitrophenyl acetate as substrate. The following metal ions, Pb(2+), Co(2+), Hg(2+), Cd(2+), Zn(2+), Se(2+), Cu(2+), Al(3+) and Mn(3+) showed inhibitory effects on these enzymes. The tested metal ions inhibited these CAs competitively in the low milimolar/submillimolar range. The susceptibility to various cations inhibitors differs significantly between these vertebrate α-CAs and is probably due to their binding to His64 or the histidine cluster. PMID:22145795

  15. Permanent genetic resources added to Molecular Ecology Resources Database 1 December 2011-31 January 2012.

    Science.gov (United States)

    Arias, M C; Arnoux, E; Bell, James J; Bernadou, Abel; Bino, Giorgia; Blatrix, R; Bourguet, Denis; Carrea, Cecilia; Clamens, Anne-Laure; Cunha, Haydée A; d'Alençon, E; Ding, Yi; Djieto-Lordon, C; Dubois, M P; Dumas, P; Eraud, C; Faivre, B; Francisco, F O; Françoso, E; Garcia, M; Gardner, Jonathan P A; Garnier, S; Gimenez, S; Gold, John R; Harris, D J; He, Guangcun; Hellemans, B; Hollenbeck, Christopher M; Jing, Shengli; Kergoat, G J; Liu, Bingfang; McDowell, Jan R; McKey, D; Miller, Terrence L; Newton, Erica; Pagenkopp Lohan, Katrina M; Papetti, Chiara; Paterson, Ian; Peccoud, J; Peng, Xinxin; Piatscheck, F; Ponsard, Sergine; Reece, Kimberly S; Reisser, Céline M O; Renshaw, Mark A; Ruzzante, Daniel E; Sauve, M; Shields, Jeffrey D; Solé-Cava, Antonio; Souche, E L; Van Houdt, J K J; Vasconcellos, Anderson; Volckaert, F A M; Wang, Shuzhen; Xiao, Jie; Yu, Hangjin; Zane, Lorenzo; Zannato, Barbara; Zemlak, Tyler S; Zhang, Chunxiao; Zhao, Yan; Zhou, Xi; Zhu, Lili

    2012-05-01

    This article documents the addition of 473 microsatellite marker loci and 71 pairs of single-nucleotide polymorphism (SNP) sequencing primers to the Molecular Ecology Resources Database. Loci were developed for the following species: Barteria fistulosa, Bombus morio, Galaxias platei, Hematodinium perezi, Macrocentrus cingulum Brischke (a.k.a. M. abdominalis Fab., M. grandii Goidanich or M. gifuensis Ashmead), Micropogonias furnieri, Nerita melanotragus, Nilaparvata lugens Stål, Sciaenops ocellatus, Scomber scombrus, Spodoptera frugiperda and Turdus lherminieri. These loci were cross-tested on the following species: Barteria dewevrei, Barteria nigritana, Barteria solida, Cynoscion acoupa, Cynoscion jamaicensis, Cynoscion leiarchus, Cynoscion nebulosus, Cynoscion striatus, Cynoscion virescens, Macrodon ancylodon, Menticirrhus americanus, Nilaparvata muiri and Umbrina canosai. This article also documents the addition of 116 sequencing primer pairs for Dicentrarchus labrax. PMID:22448966

  16. Streptococcus iniae infection in cultured tilapia, sea bass, and barramundi in Guangdong%广东省养殖罗非鱼、海鲈、尖吻鲈海豚链球菌感染调查

    Institute of Scientific and Technical Information of China (English)

    张俊; 周素明; 李安兴

    2012-01-01

    利用细菌分离培养方法结合特异PCR技术,对广东省珠三角地区养殖罗非鱼(Oreochromis spp.)、海鲈(Lateolabrax japonicus)及尖吻鲈(Lates calcarifer)的海豚链球菌(Streptococcus iniae)感染情况进行了周年调查.每月固定时间在特定养殖区域采集目标鱼的脑、肝、脾、肾和肌肉等组织,并对其进行海豚链球菌的细菌分离培养鉴定.仅从已经患病的尖吻鲈中分离到3株链球菌,经生理生化鉴定和16S rDNA测序确定为海豚链球菌.利用海豚链球菌特异PCR技术对上述养殖鱼类不同组织进行检测,发现罗非鱼、海鲈、尖吻鲈的海豚链球菌感染率分别为30.21%、23.53%、14.55%,其中罗非鱼脑和肌肉的感染率明显较其他组织高(P<0.05),分别为20.65%和23.75%;海鲈的脑部和肌肉感染率也较其他组织高(P<0.05),分别为12.1%和10%;而尖吻鲈各组织感染率没有较大差异(P>0.05).另外,研究结果还表明采集样本的海豚链球菌感染率随着其体长的增加而呈现下降趋势.%Streptococcus iniae infects a number of cultured fish species, causing heavy economic loss in Guangdong province. We evaluated the prevalence of infection with S. Iniae in tilapia {Oreochromis spp.), sea bass {Lateolabrax japonicus), and barramundi (Lates calcarifex) using a standard microbiological assay and specific PCR. Using PCR, we detected S. Iniae in all three species (30.21%, 23.53%, and 14.55% of individuals, respectively). However, S. Iniae was only isolated from three barramundi using the traditional microbiological assay. S. Iniae was detected in all tissues, including brain, liver, spleen, kidney, and muscle. The brain and muscle were more susceptible to S. Iniae than the other tissues in tilapia (infection rate: 20.65% and 23.75%, respectively) and sea bass (infection rate: 12.1% and 10%, respectively). There was no difference in the susceptibility of the tissues in barramundi. Moreover, our results

  17. Stimulation of transactivation of the largemouth bass estrogen receptors alpha, beta-a, and beta-b by methoxychlor and its mono- and bis-demethylated metabolites in HepG2 cells.

    Science.gov (United States)

    Blum, Jason L; James, Margaret O; Stuchal, Leah D; Denslow, Nancy D

    2008-01-01

    The purpose of this study was to determine the mechanisms by which the pesticide, methoxychlor (MXC), acts as an environmental endocrine disruptor through interaction with the three largemouth bass (Micropterus salmoides) estrogen receptors (ERs) alpha, betaa, and betab. MXC is a less-environmentally persistent analog of DDT that behaves as a weak estrogen. Using transient transfection assays in HepG2 cells, we have previously shown that each receptor is responsive to the endogenous ligand 17beta-estradiol (E(2)) in a dose-dependent manner. The parent compound, MXC, showed dose-dependent stimulation of transcriptional activation through all three ERs. In addition to the parent molecule, each of the metabolites was also estrogenic with all three ERs. The order of potency for ERalpha and ERbetab was HPTE>OH-MXC>MXC, while the opposite order was seen for ERbetaa. HepG2 cells did not substantially metabolize MXC to the active metabolites, thus the activity of MXC was not due to metabolism. When examining the effects of increasing concentrations of MXC at a fixed concentration of E(2), all three ERs show increased activity compared to that with E(2) alone, showing that the effects of MXC and E(2) are additive. However, when this experiment was repeated with increasing concentrations of HPTE at a fixed concentration of E(2), the activity of ERalpha was decreased, that of ERbetab was increased, while that of ERbetaa was unaffected compared to E(2) alone. These experiments suggest that HPTE functions as an E(2) antagonist with ERalpha, an E(2) agonist with ERbetab and does not perturb E(2) stimulation of ERbetaa. While it is clear the ERbeta subtypes are the products of different genes (due to a gene duplication in teleosts) the differences in their responses to MXC and its metabolites indicate that their functions diverge, both in their in vivo molecular response to E(2), as well as in their interaction with endocrine disrupting compounds found in the wild.

  18. Spatial and temporal variation in fish community of the Ria de Aveiro estuarine lagoon (Portugal.

    Directory of Open Access Journals (Sweden)

    Eva García-Seoane

    2015-11-01

    Full Text Available Fish assemblages of Ria de Aveiro coastal lagoon (Northwest Portugal were studied from February 2012 and January 2013. Sampling were monthly conducted with a traditional beach-seine net (“chincha” at 9 sites. Multivariate approach were used in order to analyse the spatial and temporal variation in fish community. A total of 58,201 fishes, weighting 249,540 g, were identified, representing 37 taxa and 21 families. Four taxa (Atherina spp., Dicentrarchus labrax, Liza spp., and Sardina pilchardus were responsible for 94.7% and 85.4% of the total abundance, in number and weight. There were significant differences in fish community, in terms of fish number and weight, between sites and between months (ANOSIM, p<0.01. A total of 12 species appeared as characteristics of one of more sampling sites either in terms of number and weight. In both cases, D. labrax, Liza spp. and Atherina spp. were typical throughout the study area. Other species, such as Spondyliosoma cantharus appeared as typical only in the part of the lagoon more influenced by marine waters, whereas Anguilla anguilla was typical of the inner area. A total of 11 (in number or 13 (in weight species appeared as typical of at least one month of the year. Liza spp. and Atherina spp. were typical all the year round for number and weight data. In both cases, D. labrax, Diplodus spp. and S. pilchardus, appeared as a typical from spring to autumn. The number of typical species varied with the season, being maximum in May and minimum in winter. Finally, results of this work were discussed in comparison to previous studies.

  19. Impact of ultraviolet-B radiation on planktonic fish larvae: Alteration of the osmoregulatory function

    Energy Technology Data Exchange (ETDEWEB)

    Sucre, Elliott, E-mail: elliott.sucre@univ-montp2.fr [AEO Team (Adaptation Ecophysiologique et Ontogenese), UMR 5119 Ecosym UM2, CNRS, IRD, Ifremer, UM1, Universite Montpellier 2, cc092, Pl. Eugene Bataillon, 34095 Montpellier, Cx 05 (France); Vidussi, Francesca [RESEAUX Team (Reseaux Planctoniques et Changement Environnemental), UMR 5119 Ecosym UM2, CNRS, IRD, Ifremer, UM1, Universite Montpellier 2, cc093, Pl. Eugene Bataillon, 34095 Montpellier, Cx 05 (France); Mostajir, Behzad [RESEAUX Team (Reseaux Planctoniques et Changement Environnemental), UMR 5119 Ecosym UM2, CNRS, IRD, Ifremer, UM1, Universite Montpellier 2, cc093, Pl. Eugene Bataillon, 34095 Montpellier, Cx 05 (France); Centre d' ecologie marine experimentale MEDIMEER (Mediterranean centre for Marine Ecosystem Experimental Research), Universite Montpellier 2-CNRS (UMS 3301), Station Mediterraneenne de l' Environnement Littoral, MEDIMEER, 2 Rue des Chantiers, 34200 Sete (France); Charmantier, Guy; Lorin-Nebel, Catherine [AEO Team (Adaptation Ecophysiologique et Ontogenese), UMR 5119 Ecosym UM2, CNRS, IRD, Ifremer, UM1, Universite Montpellier 2, cc092, Pl. Eugene Bataillon, 34095 Montpellier, Cx 05 (France)

    2012-03-15

    Coastal marine ecosystems are submitted to variations of several abiotic and biotic parameters, some of them related to global change. Among them the ultraviolet-B (UV-B) radiation (UVBR: 280-320 nm) may strongly impact planktonic fish larvae. The consequences of an increase of UVBR on the osmoregulatory function of Dicentrarchus labrax larvae have been investigated in this study. In young larvae of D. labrax, as in other teleosts, osmoregulation depends on tegumentary ion transporting cells, or ionocytes, mainly located on the skin of the trunk and of the yolk sac. As early D. labrax larvae passively drift in the top water column, ionocytes are exposed to solar radiation. The effect of UVBR on larval osmoregulation in seawater was evaluated through nanoosmometric measurements of the blood osmolality after exposure to different UV-B treatments. A loss of osmoregulatory capability occured in larvae after 2 days of low (50 {mu}W cm{sup -2}: 4 h L/20 h D) and medium (80 {mu}W cm{sup -2}: 4 h L/20 h D) UVBR exposure. Compared to control larvae kept in the darkness, a significant increase in blood osmolality, abnormal behavior and high mortalities were detected in larvae exposed to UVBR from 2 days on. At the cellular level, an important decrease in abundance of tegumentary ionocytes and mucous cells was observed after 2 days of exposure to UVBR. In the ionocytes, two major osmoeffectors were immunolocalized, the Na{sup +}/K{sup +}-ATPase and the Na{sup +}/K{sup +}/2Cl{sup -} cotransporter. Compared to controls, the fluorescent immunostaining was lower in UVBR-exposed larvae. We hypothesize that the impaired osmoregulation in UVBR-exposed larvae originates from the lower number of tegumentary ionocytes and mucous cells. This alteration of the osmoregulatory function could negatively impact the survival of young larvae at the surface water exposed to UVBR.

  20. Impact of ultraviolet-B radiation on planktonic fish larvae: Alteration of the osmoregulatory function

    International Nuclear Information System (INIS)

    Coastal marine ecosystems are submitted to variations of several abiotic and biotic parameters, some of them related to global change. Among them the ultraviolet-B (UV-B) radiation (UVBR: 280–320 nm) may strongly impact planktonic fish larvae. The consequences of an increase of UVBR on the osmoregulatory function of Dicentrarchus labrax larvae have been investigated in this study. In young larvae of D. labrax, as in other teleosts, osmoregulation depends on tegumentary ion transporting cells, or ionocytes, mainly located on the skin of the trunk and of the yolk sac. As early D. labrax larvae passively drift in the top water column, ionocytes are exposed to solar radiation. The effect of UVBR on larval osmoregulation in seawater was evaluated through nanoosmometric measurements of the blood osmolality after exposure to different UV-B treatments. A loss of osmoregulatory capability occured in larvae after 2 days of low (50 μW cm−2: 4 h L/20 h D) and medium (80 μW cm−2: 4 h L/20 h D) UVBR exposure. Compared to control larvae kept in the darkness, a significant increase in blood osmolality, abnormal behavior and high mortalities were detected in larvae exposed to UVBR from 2 days on. At the cellular level, an important decrease in abundance of tegumentary ionocytes and mucous cells was observed after 2 days of exposure to UVBR. In the ionocytes, two major osmoeffectors were immunolocalized, the Na+/K+-ATPase and the Na+/K+/2Cl− cotransporter. Compared to controls, the fluorescent immunostaining was lower in UVBR-exposed larvae. We hypothesize that the impaired osmoregulation in UVBR-exposed larvae originates from the lower number of tegumentary ionocytes and mucous cells. This alteration of the osmoregulatory function could negatively impact the survival of young larvae at the surface water exposed to UVBR.

  1. Mise en évidence d'une séquence de précipitation des sels dans les sols sulfatés acides d'une vallée aménagée de Basse-Casamance (Sénégal)

    OpenAIRE

    Montoroi, Jean-Pierre

    1995-01-01

    Au cours de la sécheresse actuelle en Afrique de l'Ouest, de nombreuses efflorescences salines se sont formées à la surface des sols sulfatés acides de Basse-Casamance, notamment dans les vallées aménagées par un barrage anti-sel. Leur caractérisation minéralogique montre qu'elles sont constituées par une grande variété de sulfates d'aluminium et de fer. Les différentes paragenèses minérales s'ordonnent dans le paysage selon une séquence de précipitation. (Résumé d'auteur)

  2. System Properties and Control of Turbocharged Diesel Engines with High-and Low-Pressure EGR Propriétés système et contrôle de moteurs Diesel turbocompressés avec EGR haute et basse pression

    Directory of Open Access Journals (Sweden)

    Mrosek M.

    2011-09-01

    Full Text Available An extension of the air-and exhaust-system with a low-pressure EGR has the potential to significantly reduce the NOx emissions. Besides a cooled high-pressure EGR and the turbocharger with variable geometry turbine, the low-pressure EGR introduces an additional degree of freedom to control the cylinder charge. This increasing complexity of the air system can be handled with model based control structures and a model based controller calibration. Its static and dynamic properties are investigated. Besides the static couplings in the classical air path, additional couplings appear. A decentralised gain scheduled PI(D-control approach is chosen to control the variables air mass flow rate, high-pressure EGR mass flow rate and charge air pressure. An automated controller calibration, based on a semi-physical mean value model of reduced complexity, is presented. The controller maps depend on the engine operation point and are calibrated by a local linearisation of the semi-physical model. Further, a semi-physical control is capable to almost solely control the air mass flow rate via the low-pressure EGR actuators. This control implicitly accounts for couplings between charge air pressure, high-pressure EGR and the low-pressure EGR system. Finally testbed results are shown. Une extension du système d’air et d’échappement au moyen d’EGR basse pression peut potentiellement réduire significativement les émissions de NOx. À côté de l’EGR haute pression refroidi et du turbocompresseur à turbine à géométrie variable, l’EGR basse pression introduit un degré de liberté supplémentaire pour contrôler la charge du cylindre. Cette complexité croissante du système d’air peut être traitée à l’aide de structures de contrôle et d’un étalonnage de régulateur basés sur des modèles. Ses propriétés statiques et dynamiques sont étudiées. En dehors des couplages statiques dans la boucle d’air classique, des couplages

  3. Sequence Analysis of DNA Methyltransferase Gene from Largemouth bass ulcerative syndrome virus and Its Expression in Prokaryote%大口黑鲈溃疡综合征病毒甲基转移酶基因序列分析与原核表达

    Institute of Scientific and Technical Information of China (English)

    马冬梅; 白俊杰; 邓国成; 李胜杰

    2011-01-01

    In order to further reveal the characteristics of Largemouth bass ulcerative syndrome virus (LBUSV)isolated recently, and further study the group of LBUSV, DFV (Doctor fish virus) and LMBV (Largemouth bass virus), the full-length DNA methyltransferase (DNA MTase) gene was analyzed and expressed using prokaryotic system.A about 200 bp core fragment was amplified and sequenced, and the full-length of DNA MTase was identified by genome walking.The open reading frame (ORF) of DNA MTase gene was 663 bp encoding 220 amino acids (GenBank accession No.GU256634).Motif analysis indicated that LBUSV DNA MTase protein contained blocks Ⅰ, Ⅳ, Ⅵ and Ⅷ, and cofactor binding sites, substrate interacting sites and DNA binding sites were also found in LBUSV DNA MTase, and the proline-proline-cysteine tripeptide was proposed catalytic site.Additionally, the primers were designed according to DNA MTase ORF, and the PCR products were inserted in vector pBV220 and transformed to host Escherichia coli DH5α.After temperature inducement and SDS-PAGE analysis, the recombinant expression bacteria produced a special protein about 25 kD in molecular weight.The proportion of recombinant protein in total bacterial protein was about 30%.Characteristic analysis of DNA MTase gene showed that the predicted protein may play a role of DNA methyltransferase, and confirmed that the taxonomic status of LBUSV is genus Ranavirus of the family Iridoviridae.%为了对新分离到的大口黑鲈溃疡综合征病毒(Largemouth bass ulcerative syndrome virus,LBUSV)的特征作进一步了解,促进LBUSV、DFV(Doctor fish virus)和LMBV(Largemouth bass vires)这类病毒的深入研究,本文克隆了LBUSV DNA甲基转移酶(DNA MTase)基因约200 bp的保守核心片段,用基因组步移的方法扩增到了核心片段的侧翼序列,获得了DNA MTase基因的编码区全长序列.序列分析表明,DNA MTase基因开放阅读框共663 bp(GenBank登录号:GU256634),编码220个氨基

  4. Contribution to the study, by magnetic resonance, of the properties of heavily doped silicon at low temperature; Contribution a l'etude par resonance magnetique des proprietes a basse temperature du silicium fortement dope

    Energy Technology Data Exchange (ETDEWEB)

    Jerome, D. [Commissariat a l' Energie Atomique, Saclay (France). Centre d' Etudes Nucleaires

    1965-10-01

    The magnetic properties of heavily doped silicon at low temperature have been studied in a range of concentration on either side of the Mott transition. For impurity densities less than that of the transition a magnetic double resonance method allows the exchange coupling between localized donors to be measured, and in addition the existence of paramagnetic centers (pairs of neutral and ionized donors) is demonstrated. The behaviour of the spin-lattice relaxation of {sup 29}Ci is explained in terms of the dipolar coupling between nuclei and paramagnetic centers. In the range of concentration 10{sup 16}-10{sup 17} impurities/cm{sup 3}, the concentration dependent relaxation of donors is studied experimentally. A theoretical explanation is proposed for the latter mechanism, the basis of which is the presence of ionized pairs of donors. At increasing impurity concentrations the electronic delocalization increases. For the concentration of 2.5 X 10{sup 18} P/cm{sup 3} the hyperfine coupling is responsible for the {sup 29}Si relaxation. It is shown that the electron density has a very large maximum near the impurities in the metallic domain of concentrations. An estimation of the impurity band width (19 deg. K) is deduced from the measurement of the paramagnetic part of the electronic susceptibility at low temperature. (author) [French] Dans ce present travail les proprietes magnetiques du silicium fortement dope a basse temperature sont etudiees dans une gamme etendue de concentrations de part et d'autre de la transition de Mott. Pour les densites d'impuretes de phosphore inferieures a celle de la transition, une methode de double resonance magnetique permet de determiner le couplage d'echange entre donneurs localises et fait ressortir l'existence de centres paramagnetiques qui sont des paires (phosphore neutre-phosphore ionise). Toutes les particularites de la relaxation nucleaire spin-reseau de {sup 29}Si s'expliquent par son couplage de

  5. Embryonic development and morphological characteristics of larval,juvenile and young Kelp bass,Epinephelus moara%云纹石斑鱼胚胎发育及仔、稚、幼鱼形态观察

    Institute of Scientific and Technical Information of China (English)

    宋振鑫; 陈超; 翟介明; 李炎璐; 马文辉; 王鲁; 庞尊方; 吴雷明

    2012-01-01

    The embryonic and morphological development of larval,juvenile and young kelp bass Epinephelus moara were observed and studied.The morphological features and the developmental phases were described in great detail from fertilization to young fish.The results showed that the time period for embryonic development is 38h 17min at 22 ±0.2℃,salinity 30,DO 7.8mg/L,and pH 8.25.Based on the obtained data,six periods including 28 stages of E.moara embryo were described,which were cleavage,blastula,gastrula,neurula,organogenesis,and hatching period.After being reared for 5 days in the sea water at 23 ±1 ℃,salinity 30 ±3,DO≥5mg/L,pH 8.0±0.5,the yolk-sac of larvae disappeared and the late larval stage began; after 27 days,the faster growing larvae developed into juveniles; after 65 days,the faster growing juveniles completed metamorphosis and became young fish.The post embryonic development of E.moara was divided into larval stage,juvenile stage as well as young fish stage,based on the features of the yolk-sac,finfold,pelvic fin spine,second dorsal fin spine,scale and body color.Accordingly,the larval stage was further classified into early larvae and late larvae,according to the existance of the yolk-sac,respectively.%对云纹石斑鱼Epinephelus moara胚胎发育及仔、稚、幼鱼形态进行了观察与研究,详细描述了各发育期的形态特征和发育时间.结果表明:1)在水温22±0.2℃、盐度30、溶氧7.8mg/L、pH 8.25的条件下,云纹石斑鱼的受精卵历时38h 17min开始孵化出膜.胚胎发育可分为受精卵、卵裂、原肠、神经胚和器官形成及出膜6个阶段,受精、胚盘形成、2细胞等28个时期.2)在水温23±1℃,盐度30±3,DO≥5mg/L,pH8.0±0.5的海水中,培育至5d,卵黄囊完全消失,成为后期仔鱼;培育至27d,发育最快的云纹石斑鱼结束仔鱼期,进入稚鱼期;培育至65d,发育最快的稚鱼完成变态,成为幼鱼.胚后发育过程主要是根据卵黄囊、

  6. Alternative protein sources in sea bass nutrition

    Directory of Open Access Journals (Sweden)

    E. D’Agaro

    2011-03-01

    Full Text Available Intensive fish farming profitability is declining word-wide. This situation is mainly due to the extensive reliance on fish meal (FM as feed ingredient in finfish diets in order to meet the high dietary protein needs. FM quality and prices are variable and its global supply is decreasing and highly dependent on meteoric events (e.g. El niño. Several protein sources are potentially excellent alternatives to FM...

  7. Smart Battery Thermal Management for PHEV Efficiency Une gestion avancée de la thermique de la batterie basse tension de traction pour optimiser l’efficacité d’un véhicule hybride électrique rechargeable

    Directory of Open Access Journals (Sweden)

    Lefebvre L.

    2013-03-01

    Full Text Available A smart battery thermal management is crucial for vehicle performances and battery lifetime targets achievements when electric and plug-in hybrid electric vehicles are concerned. The thermal system needs to be designed and tuned in accordance and compromises with powertrain and vehicle requirements, battery pack architecture, environmental constraints, costs, weight, etc., in a process that will be described in the first part of this paper. Among the portfolio of battery thermal management technologies, these items will be illustrated by two examples: thermal management by cabin air and by refrigerant in a direct cooling, enlightening a decision process. A simplified battery thermo-electric simulation model, which the second part of our work focuses on, has been built, first for both thermal and energetic balance dimensioning of the battery thermal management system. Examples are given on these two perspectives. That simplified simulation model has also identified some promising thermal management strategies for improving vehicle efficiency and performances and battery lifetime. That is the task of the last part of this paper. Battery heating has shown opportunities for improving energy and power availability at cold conditions and, thus, electric drive availability and autonomy. Post-cooling the battery at the end of a journey and its pre-conditioning before the following journey, not only improve vehicle efficiency, electric drive availability and autonomy, but also enhance battery lifetime and compromises with cabin thermal comfort. Others promising strategies optimizing the relation between vehicle performances and battery lifetime are still under investigations. L’atteinte des performances et des prestations requises d’un véhicule électrique ou hybride électrique rechargeable nécessite un thermomanagement intelligent de la batterie basse tension de traction. Ce thermomanagement est incontournable pour respecter dans le même temps

  8. The Jossey-Bass Reader on Gender in Education. The Jossey-Bass Education Series.

    Science.gov (United States)

    2002

    These papers examine various perspectives on the gender debate in education: (1) "'Too Strong for a Woman': The Five Words That Created Title IX" (Bernice R. Sandler); (2) "Feminists Discover the Hidden Injuries of Coeducation" (David Tyack and Elisabeth Hansot); (3) "Images of Relationship" (Carol Gilligan); (4) "Real Boys: The Truths behind the…

  9. Effects of Hydrolyzed Yeast on Growth Performance, Lipids Metabolism and Intestinal Structure of Largemouth Bass ( Micropterus salmoides)%酵母酶解物对大口黑鲈生长性能、脂类代谢及肠道组织结构的影响

    Institute of Scientific and Technical Information of China (English)

    郑银桦; 彭聪; 吴秀峰; 韩芳; 薛敏; 王嘉; 胡亮

    2015-01-01

    本文旨在通过研究酵母酶解物对大口黑鲈( Micropterus salmoides)生长性能、脂类代谢及肠道组织结构的影响,来评价酵母酶解物在大口黑鲈饲料中的有效性. 配制3 种等蛋白质(粗蛋白质47%)等能(总能20.0 MJ/kg)的饲料:以含35%低温干燥鱼粉的高鱼粉饲料为对照饲料(命名为FM) ,在此基础上以混合植物蛋白质(豆粕+谷朊粉)替代50%的鱼粉并平衡必需氨基酸后作为基础饲料(命名为SBM ) ,另在基础饲料中添加3%的酵母酶解物作为试验饲料(命名为HY). 选用初始体重为(20.80±0.04) g的健康大口黑鲈为试验对象,随机分为3组,每组4个重复,每个重复25尾,在室内循环控温系统中进行8周生长试验. 结果表明:在增重率、特定生长率、摄食率上,HY组显著高于FM和SBM组( P0.05). 用混合植物蛋白质替代50%鱼粉后,大口黑鲈肠道肌层变薄,绒毛完整齐度下降,在此基础上添加酵母酶解物后,肠道结构损伤的情况有所缓解. 由此得出,在本试验条件下,用混合植物蛋白质替代50%鱼粉后添加3%的酵母酶解物能够提高大口黑鲈的生长性能,改善肝脏状态,促进肝脏脂肪和胆固醇的代谢,修复肠道损伤.%This experiment was conducted to study the effects of hydrolyzed yeast on growth performance, lip?ids metabolism and intestinal structure of largemouth bass ( Micropterus salmoides) , and to evaluate the effica?cy of hydrolyzed yeast in the largemouth bass diet. In the experiment, three isoproteic ( 47% crude protein) and isoenergetic (20.0 MJ/kg gross energy) diets were formulated:a high fish meal diet containing 35% low?temperature drying fish meal was designed as control diet ( named FM) , and 50% of the fish meal was re?placed by plant protein mixture ( soybean meal+wheat gluten) with 3% hydrolyzed yeast ( named HY) or not (named SBM) on the basis of amino acid balance. Largemouth bass with the initial body weight of (20.80± 0.04) g were as

  10. 饲料中脂肪与蛋白质比对大口黑鲈生长、体组成和非特异性免疫的影响%Effects of dietary lipid to protein ratios on growth performance, body composition and non-specific immunity of largemouth bass (Micropterus salmoides)

    Institute of Scientific and Technical Information of China (English)

    陈乃松; 肖温温; 梁勤朗; 周恒永; 马秀丽; 赵明

    2012-01-01

    组成、饲料效率和免疫力有着不同程度的影响;饲料中过高的脂肪会抑制蛋白质的消化与利用,表明脂肪对蛋白质的节约作用有限.建议大口黑鲈实用饲料的蛋白质和脂肪水平分别保持在46%~49%和11.5%~14%范围内较为适当.%An 88-day feeding test was conducted to estimate the effects of dietary lipid to protein ratios on growth performance, body composition and non-specific immunity in largemouth bass with initial body weight (10.06±0.02) g. Eight diets(Dl-D8)were formulated with varying lipid to protein (LIP/PRO, w/w) ratios ranging from 0.17 to 0.84. From D1 to D8, dietary lipid levels were increased (9.0%-26.5%), while dietary protein levels were decreased (52.0%-31.0%). The results of this study suggested that the specific growth rate, feed efficiency ratio (FER), dietary protein digestibility coefficient, dietary lipid digestibility coefficient and lipid deposition of D2-fed fish were the highest. The protein efficiency ratio was significantly higher in fish fed D3-D5, compared with fish fed Dl and D8. The protein deposition rates of D2- and D3-fed fish were significantly higher than those in fish fed Dl and D5-D8. The dietary energy digestibility coefficients among the fish fed D1-D4 were significantly higher than those with other treatments. Excessive dietary lipid had a significant effect on body composition with increased body lipid deposition. The survival rates were significantly higher in fish fed Dl and D2 with higher protein levels, compared with those in fish fed D5-D8 with lower protein levels. The highest activity of lysozyme, CH50 and respiratory burst of head kidney leukocytes occurred in D4-, D2- and D2-fed fish, respectively. According to a one-way ANOVA against dietary treatment, the optimal dietary protein and lipid levels for growth and FER of largemouth bass are 49.30% and 11.50%, respectively. Using a second-order polynomial regression analysis, the optimum dietary

  11. Understanding the influence of predation on introduced fishes on juvenile salmonids in the Columbia River Basin: Closing some knowledge gaps. Late summer and fall diet and condition of smallmouth bass, walleye, and channel catfish in the middle Columbia River, USA. Interim Report of Research 2011.

    Science.gov (United States)

    Rose, Brien P.; Hansen, Gabriel S.; Weaver, Michele; Ayers, David; Van Dyke, Erick S.; Mesa, Matthew G.

    2012-01-01

    American shad Alosa sapidissima in the middle Columbia River (MCR)—a high energy food available in the summer and fall—may be contributing to the increased growth and enhanced condition of nonnative piscivores. To test this hypothesis we quantified the late summer and autumn diets of smallmouth bass Micropterus dolomieu, walleye Sander vitreus, and channel catfish Ictalurus punctatus in the three lowermost reservoirs on the Columbia River (Bonneville [BON], The Dalles [TDA], and John Day [JDA]). The diet of smallmouth bass (SMB) was fairly similar among reservoirs, with crustaceans (52–82%) and fish (13–38%) being the dominant prey groups by percent mass. Cottidae were usually the dominant fish prey in the diet of SMB at all areas and the contribution of juvenile shad ranged from 0–8.2%. Fish (mostly Cyprinidae and Cottidae) were always the dominant prey item for walleye (WAL) at all areas and at all times, ranging from 70–100% of their diet by mass. Juvenile American shad composed from 10–27% (by mass) of the diet of walleye, depending on area and month. For channel catfish (CHC), the most common prey items consumed were crustaceans (20%–80% by mass) and unidentified items (30%–80%). Fish represented a relatively small component (< 4%) of their diet. We also evaluated the condition of SMB and WAL by determining relative weights (Wr) and hepatosomatic indices (HSI). Mean Wr for SMB greater than 300 mm ranged from 0.89 to 0.94 depending on area and month and showed a significant increase from August to September for fish in BON only. Overall, mean Wr of WAL was similar at all areas, ranging from 0.89–0.91, and increased significantly from September to mid-October and November for fish in TDA only. Overall, mean HSI of SMB ranged from 1.18 to 1.48, did not differ between fish in different reservoirs, and increased significantly from September to mid-October and November for fish from the lower JDA only. Mean HSI of WAL was significantly higher in

  12. Résonance magnétique nucléaire 1H basse résolution. Le meilleur outil pour une détermination précise de la teneur en hydrogène des produits pétroliers Low Resolution 1h Nmr. The Ultimate Tool for Accurate Determination of Hydrogen Content in Petroleum Products

    Directory of Open Access Journals (Sweden)

    Gautier S.

    2006-11-01

    Full Text Available Un spectromètre RMN basse résolution à impulsions a été utilisé pour déterminer la teneur totale en hydrogène d'une large gamme de fractions pétrolières. On a constaté une excellente cohérence avec la teneur théorique en hydrogène de plusieurs composés purs; la répétabilité de la méthode est de 0,03%. La validation de cette méthode a été effectuée sur une vaste gamme de produits pétroliers comprenant notamment : distillats moyens de distillation directe, de craquage, d'hydrotraitement ou d'hydrocraquage, gazoles sous vide, bruts lourds, résidus atmosphériques de distillation directe ou d'hydrotraitement, soit au total 121 échantillons. Cette méthode s'est avérée la plus précise pour le calcul de la consommation d'hydrogène sur unités d'hydrotraitement, pour un domaine allant de 0,1 à 2,5 % pds. A low resolution pulsed NMR spectrometer has been used to determine total hydrogen content for a wide range of petroleum cuts. Excellent agreement has been found with the theoritical amount of hydrogen on pure compounds and the repeatability of the method is 0. 03%. The validation of the method has been done on a very large range of petroleum products, including straight run, cracked, hydrotreated and hydrocracked mid-distillates, vacuum gasoils, heavy crudes, straight run and hydrotreated atmospheric residues, representing 121 samples and a hydrogen consumption range during processing from 0,1 to 2. 5 wt.

  13. Freshness evaluation of sea bass using multi-sensor information fusion based on olfactory visualization and NIR spectroscopy technique%基于嗅觉可视化和近红外光谱融合技术的海鲈鱼新鲜度评价

    Institute of Scientific and Technical Information of China (English)

    黄星奕; 管超; 丁然; 吕日琴

    2015-01-01

    Total volatile basic nitrogen (TVB-N) is an important reference index of fish freshness. This study attempted to measure TVB-N content of sea bass using multi-sensor information fusion based on olfactory visualization and near-infrared spectroscopy technique. Sea bass samples were stored under the condition of 4℃ in refrigerator. The total number of samples was 270, among them 18 random samples every day were firstly detected by olfactory visualization detecting instrument, and then by near-infrared spectrometer. TVB-N content of these samples was measured according to the kjeldahl method. The experiment finished after 15 days because of the serious corruption of samples. Two-thirds of total samples were chosen as calibration set and the remaining samples were taken as prediction set using SPXY (sample set partitioning based on joint x–y distances) algorithm. So the sample sizes of calibration set and prediction set were 180 and 90 respectively. After sample division, a TVB-N prediction model was established based on the fused multi-sensor information. Other two models were also established based on the single-sensor information for comparing. Principal component analysis (PCA) method was used to reduce the dimension of colorimetric sensor array data. The results of PCA showed that the cumulative contribution rate of the first three principal components was 85.441%, which indicated that the first three principal components had been able to explain the vast majority of the overall information about original samples. Based on the first three principal components, a TVB-N prediction model was established by support vector regression (SVR) algorithm. The determination coefficients of calibration set (R2c ) and prediction set (R2p ) of the model were 0.762 and 0.757 respectively, while the root mean square errors of calibration set (RMSEC) and prediction set (RMSEP) were 6.012 and 6.755 mg/100g respectively. Mean centering (MC) method was used to preprocess the raw

  14. Low Temperature Rheological Behavior of Umbarka Waxy Crude and Influence of Flow Improver Etude du comportement rhéologique du brut paraffinique d'Umbarka à basse température et de l'influence d'un additif pour l'amélioration des caractéristiques d'écoulement

    Directory of Open Access Journals (Sweden)

    El-Gamal I. M.

    2006-12-01

    Full Text Available The low temperature flow properties; pour point and rheological parameters; have been determined for untreated and additive treated Umbarka waxy crude. The rheological characteristics of the crude showed non-Newtonian yield pseudoplastic flow behavior and pronounced thixotropic effect. Viscometric data are fitted to Bingham, Casson and Herschel-Bulkley plastic flow models using linear and non-linear computer regression analysis. Herschel parameters showed inconsistent results whereas Casson equation fitted the data reasonably well. Treatment of the crude with flow improver ameliorated both rheological properties and pour point but in a different manner. The influence of the additive on the rheology of the crude is greatly affected by shear rate. The thixotropic phenomenon of the crude is likely to be temperature-dependent in addition to the other controlling factors. L'objet de cette étude est de déterminer les caractéristiques du brut paraffinique d'Umbarka, avec et sans additif pour l'amélioration de l'écoulement, à savoir, l'écoulement à basse température, le point d'écoulement, et les données rhéologiques. Les caractéristiques rhéologiques du brut font apparaître un comportement d'écoulement pseudoplastique avec une limite élastique non newtonienne ainsi qu'un effet thixotropique accusé. Une corrélation a été établie entre les données viscométriques et les modèles d'écoulement plastique de Bingham, Casson et Herschel-Bulkley en utilisant une analyse de régression informatique linéaire et non linéaire. Si les paramètres de Herschel conduisent à des résultats incohérents, l'équation de Casson, en revanche, permet d'établir une corrélation raisonnablement satisfaisante des données. Le traitement du brut à l'aide d'un additif d'amélioration de l'écoulement a des répercussions positives, quoique différentes, sur les propriétés rhéologiques et le point d'écoulement. Les effets de l'additif sur la rh

  15. Difference in responses of two coastal species to fluctuating salinities and temperatures: Potential modification of specific distribution areas in the context of global change

    Science.gov (United States)

    Trancart, Thomas; Feunteun, Eric; Lefrançois, Christel; Acou, Anthony; Boinet, Christophe; Carpentier, Alexandre

    2016-05-01

    In the past several years, all numerical models have forecasted an increase in extreme climatic events linked to global change. Estuarine waters at the interface of marine and freshwater bodies are among the most volatile ecosystems, particularly for aquatic species, and will be strongly influenced by the temperature with extreme flooding events. This study aimed to quantify the acclimation capacity of coastal fish species to estuarine plume modifications. The thicklip mullet (Chelon labrosus) and European seabass (Dicentrarchus labrax) were selected as representative species of estuarine ecological guilds. These fish were subjected to an experiment mimicking a brief freshwater intrusion (35-5). These experiments were conducted at two different temperatures that these two species would encounter during their incursion from the sea through estuarine waters to freshwater habitats. The experimental results confirmed the high capacity for acclimation of both species to changes in salinity and temperature. Interspecific differences were observed. For example, the salinity has a greater effect on the metabolism of the seabass than on that of the mullets. Meanwhile, the temperature has a greater effect on the mullets. These differences in metabolic responses to fluctuating salinities and temperatures may modify the use of estuarine waters by these species and should be considered when predicting future specific distribution areas in the context of global change.

  16. An in vitro screening with emerging contaminants reveals inhibition of carboxylesterase activity in aquatic organisms.

    Science.gov (United States)

    Solé, Montserrat; Sanchez-Hernandez, Juan C

    2015-12-01

    Pharmaceuticals and personal care products (PPCPs) form part of the new generation of pollutants present in many freshwater and marine ecosystems. Although environmental concentrations of these bioactive substances are low, they cause sublethal effects (e.g., enzyme inhibition) in non-target organisms. However, little is known on metabolism of PPCPs by non-mammal species. Herein, an in vitro enzyme trial was performed to explore sensitivity of carboxylesterase (CE) activity of aquatic organisms to fourteen PPCPs. The esterase activity was determined in the liver of Mediterranean freshwater fish (Barbus meridionalis and Squalius laietanus), coastal marine fish (Dicentrarchus labrax and Solea solea), middle-slope fish (Trachyrhynchus scabrus), deep-sea fish (Alepocephalus rostratus and Cataetix laticeps), and in the digestive gland of a decapod crustacean (Aristeus antennatus). Results showed that 100μM of the lipid regulators simvastatin and fenofibrate significantly inhibited (30-80% of controls) the CE activity of all target species. Among the personal care products, nonylphenol and triclosan were strong esterase inhibitors in most species (36-68% of controls). Comparison with literature data suggests that fish CE activity is as sensitive to inhibition by some PPCPs as that of mammals, although their basal activity levels are lower than in mammals. Pending further studies on the interaction between PPCPs and CE activity, we postulate that this enzyme may act as a molecular sink for certain PPCPs in a comparable way than that described for the organophosphorus pesticides. PMID:26562051

  17. Linking the genomes of nonmodel teleosts through comparative genomics.

    Science.gov (United States)

    Sarropoulou, E; Nousdili, D; Magoulas, A; Kotoulas, G

    2008-01-01

    Recently the genomes of two more teleost species have been released: the medaka (Oryzias latipes), and the three-spined stickleback (Gasterosteus aculateus). The rapid developments in genomics of fish species paved the way to new and valuable research in comparative genetics and genomics. With the accumulation of information in model species, the genetic and genomic characterization of nonmodel, but economically important species, is now feasible. Furthermore, comparison of low coverage gene maps of aquacultured fish species against fully sequenced fish species will enhance the efficiency of candidate genes identification projected for quantitative trait loci (QTL) scans for traits of commercial interest. This study shows the syntenic relationship between the genomes of six different teleost species, including three fully sequenced model species: Tetraodon nigroviridis, Oryzias latipes, Gasterosteus aculateus, and three marine species of commercial and evolutionary interest: Sparus aurata, Dicentrarchus labrax, Oreochromis spp. All three commercial fish species belong to the order Perciformes, which is the richest in number of species (approximately 10,000) but poor in terms of available genomic information and tools. Syntenic relationships were established by using 800 EST and microsatellites sequences successfully mapped on the RH map of seabream. Comparison to the stickleback genome produced most positive BLAT hits (58%) followed by medaka (32%) and Tetraodon (30%). Thus, stickleback was used as the major stepping stone to compare seabass and tilapia to seabream. In addition to the significance for the aquaculture industry, this approach can encompass important ecological and evolutionary implications. PMID:18297360

  18. Increase of marine juvenile fish abundances in the middle Gironde estuary related to warmer and more saline waters, due to global changes

    Science.gov (United States)

    Pasquaud, Stéphanie; Béguer, Mélanie; Larsen, Maja Hjort; Chaalali, Aurélie; Cabral, Henrique; Lobry, Jérémy

    2012-06-01

    Estuaries have an essential ecological function for several marine fish species, that use these systems as nurseries. Previous studies in the Gironde estuary have reported an increase in water temperature and an extended seawater intrusion associated to global changes. In this study, we evaluated whether these changes impact the nursery function in the middle part of the system, based on the analyses of long-term data series (1991-2009) collected in that area. Trend analysis, correlations and generalized additive models (GAM) were computed in order to study the influence of water temperature and salinity changes on marine juveniles occurrence/densities. Results showed that densities of all the studied species (i.e. Engraulis encrasicolus, Sprattus sprattus, Dicentrarchus labrax, Solea solea, Argyrosomus regius, Merlangius merlangus) significantly increased over the period considered. Changes in water temperature and seawater intrusion contributed to explain the variability in presence/absence and in densities of the majority of these species. However, the deviance explained by GAM and the relevance of these two explanatory variables differed considerably according to species. For most species, it was suggested that the interaction of these two factors, and their effects on other variables, may explain the increase in abundance in the middle part of the Gironde. The effects of global changes in this estuarine system may favour its nursery function for marine juveniles, but some evidence of effects at a wider scale (community and ecosystem levels) may alter the structure and functioning of this system.

  19. Evidence of estuarine nursery origin of five coastal fish species along the Portuguese coast through otolith elemental fingerprints

    Science.gov (United States)

    Vasconcelos, Rita P.; Reis-Santos, Patrick; Tanner, Susanne; Maia, Anabela; Latkoczy, Christopher; Günther, Detlef; Costa, Maria José; Cabral, Henrique

    2008-08-01

    Connectivity is a critical property of marine populations, particularly for species with segregated juvenile and adult habitats. Knowledge of this link is fundamental in understanding population structure and dynamics. Young adults of commercially important fish species Solea solea, Solea senegalensis, Platichthys flesus, Diplodus vulgaris and Dicentrarchus labrax were sampled off the Portuguese coast in order to establish preliminary evidence of estuarine nursery origins through otolith elemental fingerprints. Concentrations of Li, Na, Mg, K, Mn, Cu, Zn, Sr, Ba and Pb in the otolith section corresponding to juvenile's nursery life period were determined through laser ablation inductively coupled plasma mass spectrometry. Element: Ca ratios in coastal fish differed significantly amongst collection areas, except for Platichthys flesus, and were compared with the elemental fingerprints previously defined for age 0 juveniles in the main estuarine nurseries of the Portuguese coast. Identification of nursery estuaries was achieved for four of the species. Assigned nursery origins varied amongst species and differences in the spatial scale of fish dispersal were also found. Diplodus vulgaris was not reliably assigned to any of the defined nurseries. Overall, results give evidence of the applicability of estuarine habitat tags in future assessments of estuarine nursery role. Research developments on the links between juvenile and adult habitats should contribute for the integrated management and conservation of nurseries and coastal stocks.

  20. Uncommon functional properties of the first piscine 26S proteasome from the Antarctic notothenioid Trematomus bernacchii.

    Science.gov (United States)

    Gogliettino, Marta; Balestrieri, Marco; Riccio, Alessia; Facchiano, Angelo; Fusco, Carmela; Palazzo, Vincenzo Cecere; Rossi, Mosè; Cocca, Ennio; Palmieri, Gianna

    2016-01-01

    Protein homoeostasis is a fundamental process allowing the preservation of functional proteins and it has a great impact on the life of the Antarctic organisms. However, the effect of low temperatures on protein turnover is poorly understood and the cold-adaptation of the degradation machinery remains an unresolved issue. As the 26S proteasome represents the main proteolytic system devoted to the controlled degradation of intracellular proteins, the purpose of the present study was to investigate the functions of this complex in the notothenioid Trematomus bernacchii, in order to better understand its role in the physiology of Antarctic fish. To this aim, we purified and characterized the 26S proteasome from T. bernacchii and isolated the cDNAs codifying seven of the 14 subunits belonging to the proteasome 20S core particle. Results provided evidences of the high resistance of the piscine 26S proteasome to oxidative agents and of its 'uncommon' ability to efficiently hydrolyse oxidized bovine serum albumin (BSA), suggesting that this enzymatic complex could play a key role in the antioxidant defense systems in fish inhabiting permanently cold marine environments. These unique properties were also reflected by the 3D model analysis, which revealed a higher structural stability of the piscine complex respect to the murine template. Finally, a comparative analysis, performed in a variety of tissues collected from T. bernacchii and the temperate fish Dicentrarchus labrax, showed a lower protein retention in the cold-adapted fish, possibly due to a better efficiency of its degradation machinery.