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Sample records for bass cichla sp

  1. Feed training of peacock bass (Cichla sp.)

    OpenAIRE

    M.A.M Moura; KUBITZA F.; CYRINO J. E. P.

    2000-01-01

    The Amazonian cichlid peacock bass (Cichla sp.) is a highly marketable food and sport fish, therefore a suitable species for aquaculture. However, because of its piscivorous feeding preferences, the species does not accept dry feeds voluntarily, turning its intensive culture difficult and costly. This study aimed to wean fingerling peacock bass from inert moist food to dry diets. In a first experiment, 1,134 fingerlings weighting 0.27 g were divided in two 0.37 m³ hapas and fed ground fish fl...

  2. Feed training of peacock bass (Cichla sp.

    Directory of Open Access Journals (Sweden)

    MOURA M. A. M.

    2000-01-01

    Full Text Available The Amazonian cichlid peacock bass (Cichla sp. is a highly marketable food and sport fish, therefore a suitable species for aquaculture. However, because of its piscivorous feeding preferences, the species does not accept dry feeds voluntarily, turning its intensive culture difficult and costly. This study aimed to wean fingerling peacock bass from inert moist food to dry diets. In a first experiment, 1,134 fingerlings weighting 0.27 g were divided in two 0.37 m³ hapas and fed ground fish flesh with 35% success. Then, 1.3 g fish were pooled, stocked in six 25 L cages and fed two pellet sequences with 80%, 60%, 40%, 20% and 0% ground fish flesh (GFF. One sequence was flavored with 10% krill meal (Euphausia sp.. Training success of fish fed the GFF-00 diet flavored with krill reached 12%ª compared to 11.6%ª (p < 0.05 for diets without krill meal. A second experiment was set up with 969, 1.5 g fish, trained with GFF with 39.8% success. After the feed training period, 2.2 g fish were then fed a sequence of moist pellets containing 80%, 60% and 45% GFF. Fish trained to feed on moist pellets with 45% ground fish were pooled and stocked into nine 25 L cages. Fish were weaned to dry pellets without ground fish flesh (GFF-00 using three diet sequences: 1 dry pellets; 2 moist pellets; and 3 dry pellets flavored with 4% cod liver oil; all three diets contained 30, 10 and 0% GFF. The three sequences yielded, respectively 30.8%ª, 23.6%ª, and 24.7%ª (p < 0.05 fish feeding on GFF-00. There were no apparent beneficial effects of increasing moisture or addition of cod liver oil as flavor enhancers in the weaning diets. This study revealed the feasibility of training peacock bass to accept dry pellets, but feeding young fish ground fish flesh seemed to be a major bottleneck in improving feed training success.

  3. Protease exógena em dietas para juvenis de tucunaré-paca (Cichla sp. Exogenous protease in diets for tucunaré paca (Cichla sp. juvenile

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    Emerson Carlos Soares

    2008-06-01

    Full Text Available Neste experimento foi analisada a atividade enzimática da protease exógena nos tratos digestórios dos peixes e, posteriormente, o desempenho zootécnico dos juvenis de tucunaré-paca (Cichla sp. por intermédio da inclusão de três níveis de protease exógena na dieta com 40% proteína bruta mais a dieta controle. Para avaliar o melhor nível de inclusão de enzimas, foram elaboradas quatro dietas em forma de peletes com quatro níveis de inclusão de protease exógena (0,00; 0,05; 0,10 e 0,15%, em um delineamento inteiramente casualisado (fatorial 4 × 4. Os melhores resultados de ganho de peso, conversão alimentar e crescimento específico em peso foram apresentados pelos animais alimentados com a dieta contendo 0,10% de protease exógena. Concluiu-se que a enzima protease exógena adicionada à dieta influenciou os parâmetros corpóreos dos juvenis de tucunaré-paca.This experiment analyzed the enzymatic activity of the exogenous protease in the fish digestive tract and, afterwards, it was also evaluated the growth performance of tucunaré paca (Cichla sp. juvenile by the addition of four exogenous protease levels to a control diet with 40% CP. To evaluate the best enzyme inclusion level, four pelleted diets were elaborated with the following exogenous protease levels (0.00, 0.05, 0.10 and 0.15%, in a completely randomized design in a 4 × 4 factorial arrangement. The best results of average weight gain, feed conversion ratio, and weight specific growth rate were showed by the animals fed with diet containing 0.10% of exogenous protease. The exogenous protease enzyme added to the diet affected the body characteristics of tucunaré paca juvenile.

  4. Mercury Bioaccumulation in the Brazilian Amazonian Tucunares (Cichla sp., Cichlidae, Perciformes

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    Maria Josefina Reyna Kurtz

    2008-08-01

    Full Text Available There are emissions of mercury to the atmosphere, soil and rivers of the Brazilian Amazon stem from many sources. Once in the atmosphere, the metal is oxidized and immediately deposited. In the water, the transformation to methylmercury takes place mostly by the action of microorganisms. The formation of methylmercury increases the dispersion and bioavailability of the element in the aquatic environment. Methylmercury can be assimilated by plankton and enters the food chain. The concentration of mercury increases further up in the trophic levels of the chain and reaches the highest values in carnivorous fishes like tucunare. Therefore, mercury emissions cause the contamination of natural resources and increase risks to the health of regular fish consumers. The objective of this work was to study the bioaccumulation of mercury in tucunares (Cichla sp., top predators of the food chain. The fishes were collected at two locations representative of the Amazonian fluvial ecosystem, in the state of Pará, Brazil, in 1992 and 2001. One location is near a former informal gold mining area. The other is far from the mining area and is considered pristine. Average values of total mercury concentration and accumulation rates for four different collection groups were compared and discussed. Tucunares collected in 2001 presented higher mercury contents and accumulated mercury faster than tucunares collected in 1992 notwithstanding the decline of mining activities in this period. The aggravation of the mercury contamination with time not only in an area where informal gold mining was practiced but also far from this area is confirmed.

  5. Kinetic and physicochemical properties of brain acetylcholinesterase from the peacock bass (Cichla ocellaris) and in vitro effect of pesticides and metal ions.

    Science.gov (United States)

    Silva, Kaline Catiely Campos; Assis, Caio Rodrigo Dias; Oliveira, Vagne Melo; Carvalho, Luiz Bezerra; Bezerra, Ranilson Souza

    2013-01-15

    Brain acetylcholinesterase (AChE; EC 3.1.1.7) from peacock bass (Cichla ocellaris) was characterized and the effect of organophosphorus and carbamate pesticides as well as ions and heavy metals was evaluated. The kinetic parameters K(m) and V(max) were determined as 0.769 mM and 0.189 U/mg of protein respectively. Optimal pH and temperature were found to be 8.0 and 45°C. The enzyme retained approximately half of the activity after incubation at 50°C for 30 min. Total cholinesterase activity on brain of this species can be ascribed to AChE according to selective inhibitors analysis (neostigmine, eserine and BW284c5 reduced its activity whereas no effect was noticed for Iso-OMPA). Seven pesticides (five organophosphates: dichlorvos, diazinon, chlorpyrifos, temephos, tetraethyl pyrophosphate - TEPP and two carbamates: carbaryl and carbofuran) showed inhibitory effects on C. ocellaris AChE. However, the strongest effect was observed with carbofuran (IC(50)=0.21 μM and K(i)=2.57 × 10(-3) μM). The following ions (1 mM) showed to inhibit its activity (decrescent order): Hg(2+)>As(3+)>Cu(2+)>Zn(2+). EDTA(2-) did not affect enzyme activity. The present study provides assay conditions and data to suggest this enzyme as in vitro biomarker of organophosphorus and carbamate pesticides in routine environmental screening programs. PMID:23220411

  6. Prospecting molecular markers to distinguish Cichla kelberi, C. monoculus and C. piquiti

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    Luciano Seraphim Gasques

    2015-10-01

    Full Text Available Peacock bass, a fish of the genus Cichla, is an exotic species from the upper river Paraná floodplain in which the species Cichla kelberi and C. piquiti have been confirmed, coupled to the specie C. monoculus upstream in the Capivara and Taquaruçu dams. The introduction of this genus has caused negative impacts on the diversity of native species. Current research prospects DNA sequences capable of distinguishing the three species and provide molecular data for the taxonomic characterization of the species in the upper Paraná River basin.  Sequencing of nuclear (tmo4c4, dlx2 and bmp4 and mitochondrial (cox1, cytb loci were done from fish of the three species of the genus Cichla reported in the literature of the upper Paraná River basin. Sequence analysis provided molecular differentiation for the species through the usage of loci cytb, dlx2 and cox1. Since the latter only distinguished C. piquiti from the other Cichla species, the loci bmp4 and tmo4c4 were not adequate to accomplish our aim.

  7. Streptococcus iniae infection in cultured Asian sea bass (Lates calcarifer and red tilapia (Oreochromis sp. in southern Thailand

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    Kidchakan Supamattaya

    2010-08-01

    Full Text Available Streptococcal infections are becoming an increasing problem in aquaculture and have been reported worldwide in avariety of fish species. Here we describe the isolation and characterization of Streptococcus iniae from Asian sea bass (Latescalcarifer and red tilapia (Oreochromis sp. cultured in southern Thailand. Conventional and rapid identification systems,as well as the polymerase chain reaction (PCR, were used to determine that the isolate was S. iniae. The virulence of thisS. iniae was higher in Asian sea bass than in red tilapia, as shown by the 10 day-LD50 in Asian sea bass and red tilapia, being1.08x104 and 1.14x107 CFU, respectively. Histopathological changes in Asian sea bass are more severe than those observedin red tilapia. The changes can be found in several organs including liver, pancreas, heart, eye and brain. Histopathologicalfindings included cellular necrosis, infiltration of lymphocytes and granuloma formation in the infected organs.

  8. Production of killed vaccine from Streptococcus sp. and its application in sea bass (Lates calcarifer

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    Wanman, CH.

    2007-09-01

    Full Text Available Fish with an average length of 3.0-4.0 inches vaccinated with formalin-killed vaccine of Streptococcus sp. either by intraperitoneal injection and immersion methods had a survival rate of 100% indicating that the vaccine is safe to be used with sea bass. The highest efficacy was received when the vaccine containing bacterin at 2.50x1010 CFU/ml. Injection of vaccine together with adjuvant (CFA was highly effective against Streptococcus sp. infection. The relative percent survival (RPS of fish injected with vaccine alone and vaccine plus adjuvant were 100 (10 days post vaccination, 54.06 and 92.29 (20 days post vaccination and 31.58 and 73.68 (30 days post vaccination respectively. The fish which received vaccine by hyperosmotic immersion showed higher resistance to the disease than by direct immersion with the RPS of 30.77 and 71.80 (10 days post vaccination, 9.75 and 70.73 (20 days post vaccination and 7.14 and 16.67 (30 days post vaccination for direct immersion and hyperosmotic immersion, respectively.

  9. Tenacibaculum dicentrarchi sp. nov., a marine bacterium of the family Flavobacteriaceae isolated from European sea bass.

    Science.gov (United States)

    Piñeiro-Vidal, Maximino; Gijón, Daniel; Zarza, Carles; Santos, Ysabel

    2012-02-01

    A novel Gram-stain-negative rod-shaped gliding bacterial strain, designated 35/09(T), was isolated from diseased European sea bass (Dicentrarchus labrax L.) in Spain. Colonies were pale-yellow-pigmented with uneven edges and did not adhere to the agar. The DNA G+C content of the isolate was 31.3 mol%. 16S rRNA gene sequence analysis indicated affiliation to the genus Tenacibaculum (family Flavobacteriaceae, phylum 'Bacteroidetes'). Sequence similarities between the isolate and type strains of other members of the genus were 93.1-97.3 %. The major fatty acids (>5 % of the total fatty acids) were iso-C(15 : 0) (24.8 %), iso-C(15 : 0) 3-OH (18.0 %), anteiso-C(15 : 0) (8.1 %), C(15 : 1)ω6c (6.9 %) and iso-C(15 : 1) (6.2 %). Genotypic and phenotypic data indicate that strain 35/09(T) should be classified as a representative of a novel species in the genus Tenacibaculum, for which the name Tenacibaculum dicentrarchi sp. nov. is proposed; the type strain is 35/09(T) ( = CECT 7612(T) = NCIMB 14598(T)). PMID:21460137

  10. Pathogenicity of Aeromonas hydrophila isolated from the Malaysian Sea against coral (Turbinaria sp.) and sea bass (Lates calcarifer).

    Science.gov (United States)

    Hamid, Rahimi; Ahmad, Asmat; Usup, Gires

    2016-09-01

    A study was carried out to determine the pathogenicity (hemolytic activity) on corals (Turbinaria sp.) and sea bass (Lates calcarifer) of Aeromonas hydrophila from water, sediment, and coral. Samples were collected from coastal water and coral reef areas. One hundred and sixty-two isolates were successfully isolated. Out of 162, 95 were from seawater, 49 from sediment, and 18 from coral. Sixteen isolates were picked and identified. Isolates were identified using a conventional biochemical test, the API 20NE kit, and 16S rRNA nucleotide sequences. Hemolytic activity was determined. Out of 16 isolates, 14 isolates were β-hemolytic and two isolates were non-hemolytic. Corals infected with A. hydrophila suffered bleaching. Similar effect was observed for both hemolytic and non-hemolytic isolates. Intramuscular injection of A. hydrophila into sea bass resulted in muscular bleeding and death. Higher infection rates were obtained from hemolytic compared to non-hemolytic strains of A. hydrophila isolates. PMID:27221587

  11. Characterization of Exopolysaccharides Produced by Bacillus cereus and Brachybacterium sp. Isolated from Asian Sea Bass (Lates calcarifer

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    Mohamed Orsod

    2012-09-01

    Full Text Available Aims: EPS extracted from marine bacteria, which associated with Asian sea bass has potential antimicrobial activities.Methodology and Results: Two marine Bacteria were isolated from Asian sea bass (Lates calcarifer obtained from aquaculture farm, located at Johor bahru Malaysia. 16S rRNA analysis for bacteria identity revealed that bacteria ors1 had 99 % identity to Bacillus cereus and ors2 had 96 % identity with Brachybacterium sp. All bacteria shared many similarities and variation in terms of biochemical reactions and microscopic observation. Exopolysaccharides (EPSs were extracted and purified from bacteria as they produced mucous colonies. Average analysis of EPS components showed 50 % carbohydrates, 26 % protein and 24 % fatty acids. The FTIR analysis confirmed the functional groups of the EPS. Screening for antimicrobial activities assays using Kirby-Bauer methods against both grams positive and negative had shown presence of inhibition zones.Conclusion, significance and impact of study: This study recommends that bacteria isolated from Asian sea bass are having antimicrobial activities and could be used as a potential source for the development of marine drugs.

  12. Determination of essential and toxic elements in sea bass (Centropomus sp.) and hake (Cynoscion leiarchus) consumed in the city of Sao Paulo by neutron activation analysis

    International Nuclear Information System (INIS)

    The goal was to use the Instrumental Neutron Activation Analysis (INAA) to evaluate the levels of essential and toxic elements in samples of hake (Cynoscion leiarchus) and sea bass (Centropomus sp.) that are most consumed fish in the town of some Sao Paulo. Ten specimens of hake and sea bass were acquired in Companhia de Entrepostos e Armazens Gerais de Sao Paulo (CEAGESP), main supply center for the population of the metropolitan area of Sao Paulo. After gutted and cleaned, the edible tissue was freeze-dried, ground and sieved. The elements were determined through the use of hyperpure germanium spectrometer after irradiation in the nuclear research reactor IEAR-1

  13. Dieta do tucunaré-amarelo Cichla monoculus (Bloch & Schneider (Osteichthyes, Cichlidae, no Reservatório de Lajes, Rio de Janeiro, Brasil Diet of Cichla monoculus (Bloch & Schneider (Osteichthyes, Cichlidae in Lajes' Reservoir, Rio de Janeiro, Brazil

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    Luciano Neves dos Santos

    2001-07-01

    Full Text Available The diet of Cichla monoculus (Bloch & Schneider, 1801 in Lajes's Reservoir, a major impoundment in Rio de Janeiro State, Brazil, was assessed, from fishes collected in 1994,1996 and 1999/2000. Gut contents in individuals was analyzed by the index of relative importance (IRI which deals with numerical, gravimetrical and frequency of occurrence. Cichla monoculus showed a strong piscivorous habits feeding on Cichlidae, Characidae and Pimelodidae, in decreasing order of importance, with a remarkable cannibalism on young-of-the-year. Others minor items in the diet were Macrobrachium sp. and Odonata. Changes in feeding composition varied with reservoir's zones and seasons, with higher diversity in Autumn and peaks of cannibalism in lower zone during Spring/Summer. Overall, only one third of fish species composition in the reservoir are predated by C. monoculus. Condition factor (k and fullness index varied closely with higher values in lower zone, and lower records in Winter.

  14. Seven bass drawing, Highlanders

    OpenAIRE

    Helmlinger, Aurélie

    2011-01-01

    Seven bass drawing, Highlanders panyard, Cor. EMR William sts, Success Village, Laventille( erreur dans le nom de fichier : " highlanders_nine_bass_drawing_1" ; nom correct ="highlanders_seven_bass_drawing_1 " )

  15. Dieta do tucunaré-amarelo Cichla monoculus (Bloch & Schneider) (Osteichthyes, Cichlidae), no Reservatório de Lajes, Rio de Janeiro, Brasil Diet of Cichla monoculus (Bloch & Schneider) (Osteichthyes, Cichlidae) in Lajes' Reservoir, Rio de Janeiro, Brazil

    OpenAIRE

    Luciano Neves dos Santos; Alejandra Filippo Gonzalez; Francisco Gerson de Araújo

    2001-01-01

    The diet of Cichla monoculus (Bloch & Schneider, 1801) in Lajes's Reservoir, a major impoundment in Rio de Janeiro State, Brazil, was assessed, from fishes collected in 1994,1996 and 1999/2000. Gut contents in individuals was analyzed by the index of relative importance (IRI) which deals with numerical, gravimetrical and frequency of occurrence. Cichla monoculus showed a strong piscivorous habits feeding on Cichlidae, Characidae and Pimelodidae, in decreasing order of importance, with a remar...

  16. Glucose turnover in kelp bass (Paralabrax sp.): in vivo studies with [6-3H,6-14C]glucose

    International Nuclear Information System (INIS)

    [6-3H,6-14C]glucose was injected via an indwelling arterial cannula in free-swimming, fed, and fasted kelp bass to determine hepatic glucose production, peripheral glucose uptake, minimal glucose mass, mean transit time, and the percent of carbon recycling under the two different nutritional states. Mean plasma glucose levels remained unchanged in fed and fasted fish (48 +- 8 vs. 43 +- 8 mg/100 ml). During steady-state conditions, glucose replacement rates of fed and fasted fish determined with [6-3H]glucose are similar (0.035 +- 0.006 vs. 0.025 +- 0.003 mg/min per 100 g) and do not differ from rates determined with [6-14C]glucose (0.035 +- 0.005 vs. 0.026 +- 0.002). The minimal glucose masses and the mean transit times determined with both isotopes are also similar suggesting that plasma glucose levels and glucose turnover are maintained in fish fasted up to 40 days with no apparent increase in carbon recycling. Nonsteady-state isotope experiments suggest that these fish can alter rates of hepatic glucose production and peripheral uptake in response to hyper- and hypoglycemia

  17. Hierarchical Bass model

    CERN Document Server

    Tashiro, Tohru

    2013-01-01

    We propose a new model about diffusion of a product which includes a memory of how many adopters or advertisements a non-adopter met, where (non-)adopters mean people (not) possessing the product. This effect is lacking in the Bass model. As an application, we utilize the model to fit the iPod sales data, and so the better agreement is obtained than the Bass model.

  18. Hierarchical Bass model

    Science.gov (United States)

    Tashiro, Tohru

    2014-03-01

    We propose a new model about diffusion of a product which includes a memory of how many adopters or advertisements a non-adopter met, where (non-)adopters mean people (not) possessing the product. This effect is lacking in the Bass model. As an application, we utilize the model to fit the iPod sales data, and so the better agreement is obtained than the Bass model.

  19. Hierarchical Bass model

    International Nuclear Information System (INIS)

    We propose a new model about diffusion of a product which includes a memory of how many adopters or advertisements a non-adopter met, where (non-)adopters mean people (not) possessing the product. This effect is lacking in the Bass model. As an application, we utilize the model to fit the iPod sales data, and so the better agreement is obtained than the Bass model

  20. Lower biodiversity of native fish but only marginally altered plankton biomass in tropical lakes hosting introduced piscivorous Cichla cf. ocellaris

    DEFF Research Database (Denmark)

    Menezes, Rosemberg; Attayde, José Luiz; Lacerot, Gissell; Kosten, Sarian; Coimbra e Souza, Leonardo; S. Costa, Luciana; H. Van Nes, Egbert; Jeppesen, Erik

    2012-01-01

    We compared the species richness and abundance of fish, zooplankton and phytoplankton in nine mesotrophic coastal shallow lakes (Northeastern Brazil) with and without the exotic predator cichlid tucunaré or ‘peacock bass’ (Cichla cf. ocellaris). We hypothesized that the introduction of tucunaré w...

  1. Methylmercury in a predatory fish (Cichla spp.) inhabiting the Brazilian Amazon

    Energy Technology Data Exchange (ETDEWEB)

    Kehrig, Helena do A [Laboratorio de Radioisotopos Eduardo Penna Franca, Instituto de Biofisica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro (UFRJ), 21941-902 Rio de Janeiro (Brazil)], E-mail: kehrig@biof.ufrj.br; Howard, Bruce M. [T.H. Huxley School, Imperial College, London SW7 2BP (United Kingdom); Malm, Olaf [Laboratorio de Radioisotopos Eduardo Penna Franca, Instituto de Biofisica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro (UFRJ), 21941-902 Rio de Janeiro (Brazil)

    2008-07-15

    This research tested whether limnological conditions, biological characteristics of fish and anthropogenic impacts influenced the assimilation of methylmercury into the muscle of a sedentary piscivorous fish, Cichla spp., from three rivers (Negro, Madeira, Tapajos) and two hydroelectric reservoirs (Balbina, Tucurui) within the Brazilian Amazon. Methylmercury in this fish ranged from 0.04 to 1.43 {mu}g g{sup -1} w.w. across sites. No significant differences were observed in the methylmercury concentrations between males and females, or for different morphotypes of this species. Positive correlations were found between methylmercury and fish body weight. No differences were found between the weight normalized methylmercury (MeHg) concentrations or its percent of total mercury in fish from the three rivers; weight normalized MeHg was highest in one of the two reservoirs. In Rio Tapajos, where gold mining and deforestation cause high water turbidity, fish showed the highest MeHg and concentrations were different across the four sites examined. In all sampling areas, the %MeHg was found to be higher than 70. - Cichla spp. may be considered good bioindicators of methylmercury contamination in the Amazonian ecosystem because of their integration of this pollutant over time.

  2. Methylmercury in a predatory fish (Cichla spp.) inhabiting the Brazilian Amazon

    International Nuclear Information System (INIS)

    This research tested whether limnological conditions, biological characteristics of fish and anthropogenic impacts influenced the assimilation of methylmercury into the muscle of a sedentary piscivorous fish, Cichla spp., from three rivers (Negro, Madeira, Tapajos) and two hydroelectric reservoirs (Balbina, Tucurui) within the Brazilian Amazon. Methylmercury in this fish ranged from 0.04 to 1.43 μg g-1 w.w. across sites. No significant differences were observed in the methylmercury concentrations between males and females, or for different morphotypes of this species. Positive correlations were found between methylmercury and fish body weight. No differences were found between the weight normalized methylmercury (MeHg) concentrations or its percent of total mercury in fish from the three rivers; weight normalized MeHg was highest in one of the two reservoirs. In Rio Tapajos, where gold mining and deforestation cause high water turbidity, fish showed the highest MeHg and concentrations were different across the four sites examined. In all sampling areas, the %MeHg was found to be higher than 70. - Cichla spp. may be considered good bioindicators of methylmercury contamination in the Amazonian ecosystem because of their integration of this pollutant over time

  3. Mercury Bioaccumulation in the Brazilian Amazonian Tucunares (Cichla sp., Cichlidae, Perciformes)

    OpenAIRE

    Maria Josefina Reyna Kurtz; Zuleica Carmen Castilhos; Ysrael Marrero Vera; Roberto José de Carvalho

    2008-01-01

    There are emissions of mercury to the atmosphere, soil and rivers of the Brazilian Amazon stem from many sources. Once in the atmosphere, the metal is oxidized and immediately deposited. In the water, the transformation to methylmercury takes place mostly by the action of microorganisms. The formation of methylmercury increases the dispersion and bioavailability of the element in the aquatic environment. Methylmercury can be assimilated by plankton and enters the food chain. The concentration...

  4. The Flexible Bass Absorber

    DEFF Research Database (Denmark)

    Adelman-Larsen, Niels Werner; Thompson, Eric Robert; Gade, Anders Christian

    2007-01-01

    Multi-purpose concert halls face a dilemma. They host different performance types that require significantly different acoustic conditions in order to provide the best sound quality to both the performers, sound engineers and the audience. Pop and rock music often contain high levels of bass sound...

  5. The Flexible Bass Absorber

    DEFF Research Database (Denmark)

    Adelman-Larsen, Niels Werner; Thompson, Eric Robert; Gade, Anders Christian

    2007-01-01

    Multi-purpose concert halls face a dilemma. They host different performance types that require significantly different acoustic conditions in order to provide the best sound quality to both the performers, sound engineers and the audience. Pop and rock music often contains high levels of bass sound...

  6. Tenor bass, dr02, Central Symphony

    OpenAIRE

    Helmlinger, Aurélie

    2011-01-01

    Tenor bass, dr02, augmented, Central Symphony Steel Orchestra panyard, Government House road, Tobago( erreur dans le nom de fichier : "central_symphony_steel_tenor_bass_1_dr02" ; nom correct ="central_symphony_tenor_bass_1_dr02 " )

  7. Nine bass drawing, Tropical Angel Harps

    OpenAIRE

    Helmlinger, Aurélie

    2011-01-01

    Nine bass drawing, one tone per drum, Tropical Angel Harps panyard, Southern Main Rd, Enterprise, Chaguanas (erreur sur le nom de fichier : " claytones_nine_bass_drawing_1" ; nom correct = " tropical_angel_harps_nine_bass_drawing_1")

  8. Seven bass drawing, Tropical Angel Harps

    OpenAIRE

    Helmlinger, Aurélie

    2011-01-01

    Seven bass drawing, whole tone style, Tropical Angel Harps panyard, Southern Main Rd, Enterprise, Chaguanas (erreur sur le nom de fichier : " claytones_seven_bass_drawing_1" ; nom correct = " topical_angel_harps_seven_bass_drawing_1")

  9. Feeding and reproductive ecology of Cichla piquiti Kullander & Ferreira, 2006 within its native range, Lajeado reservoir, rio Tocantins basin

    OpenAIRE

    Vanilcia Clementino de Oliveira Marto; Alberto Akama; Fernando Mayer Pelicice

    2015-01-01

    Cichla piquiti is endemic to the Tocantins-Araguaia river basin, but information about its biology is restricted to populations introduced in other basins. In order to fill this gap, we investigated the feeding ecology and reproductive dynamics of C. piquitiin Lajeado reservoir, rio Tocantins. A total of 270 fish were collected between November 2010 and October 2011. The diet is rich and composed of at least 23 resources, predominantly fish (at least 19 species). We observed no clear influenc...

  10. Some Model Theoretic Remarks on Bass Modules

    OpenAIRE

    E. Momtahan

    2011-01-01

    We study Bass modules, Bass rings, and related concepts from a model theoretic point of view. We observe that the class of Bass modules (over a fixed ring) is not stable under elementary equivalence. We observe that under which conditions the class of Bass rings are stable under elementary equivalence.

  11. Vibrio lentus protects gnotobiotic sea bass (Dicentrarchus labrax L.) larvae against challenge with Vibrio harveyi.

    Science.gov (United States)

    Schaeck, M; Duchateau, L; Van den Broeck, W; Van Trappen, S; De Vos, P; Coulombet, C; Boon, N; Haesebrouck, F; Decostere, A

    2016-03-15

    Due to the mounting awareness of the risks associated with the use of antibiotics in aquaculture, treatment with probiotics has recently emerged as the preferred environmental-friendly prophylactic approach in marine larviculture. However, the presence of unknown and variable microbiota in fish larvae makes it impossible to disentangle the efficacy of treatment with probiotics. In this respect, the recent development of a germ-free culture model for European sea bass (Dicentrarchus labrax L.) larvae opened the door for more controlled studies on the use of probiotics. In the present study, 206 bacterial isolates, retrieved from sea bass larvae and adults, were screened in vitro for haemolytic activity, bile tolerance and antagonistic activity against six sea bass pathogens. Subsequently, the harmlessness and the protective effect of the putative probiotic candidates against the sea bass pathogen Vibrio harveyi were evaluated in vivo adopting the previously developed germ-free sea bass larval model. An equivalence trial clearly showed that no harmful effect on larval survival was elicited by all three selected probiotic candidates: Bacillus sp. LT3, Vibrio lentus and Vibrio proteolyticus. Survival of Vibrio harveyi challenged larvae treated with V. lentus was superior in comparison with the untreated challenged group, whereas this was not the case for the larvae supplemented with Bacillus sp. LT3 and V. proteolyticus. In this respect, our results unmistakably revealed the protective effect of V. lentus against vibriosis caused by V. harveyi in gnotobiotic sea bass larvae, rendering this study the first in its kind. PMID:26931390

  12. The Casiquiare river acts as a corridor between the Amazonas and Orinoco river basins: biogeographic analysis of the genus Cichla.

    Science.gov (United States)

    Willis, S C; Nunes, M; Montaña, C G; Farias, I P; Ortí, G; Lovejoy, N R

    2010-03-01

    The Casiquiare River is a unique biogeographic corridor between the Orinoco and Amazonas basins. We investigated the importance of this connection for Neotropical fishes using peacock cichlids (Cichla spp.) as a model system. We tested whether the Casiquiare provides a conduit for gene flow between contemporary populations, and investigated the origin of biogeographic distributions that span the Casiquiare. Using sequences from the mitochondrial control region of three focal species (C. temensis, C. monoculus, and C. orinocensis) whose distributions include the Amazonas, Orinoco, and Casiquiare, we constructed maximum likelihood phylograms of haplotypes and analyzed the populations under an isolation-with-migration coalescent model. Our analyses suggest that populations of all three species have experienced some degree of gene flow via the Casiquiare. We also generated a mitochondrial genealogy of all Cichla species using >2000 bp and performed a dispersal-vicariance analysis (DIVA) to reconstruct the historical biogeography of the genus. This analysis, when combined with the intraspecific results, supports two instances of dispersal from the Amazonas to the Orinoco. Thus, our results support the idea that the Casiquiare connection is important across temporal scales, facilitating both gene flow and the dispersal and range expansion of species. PMID:20149086

  13. BASS Code Development

    Science.gov (United States)

    Sawyer, Scott

    2004-01-01

    The BASS computational aeroacoustic code solves the fully nonlinear Euler equations in the time domain in two-dimensions. The acoustic response of the stator is determined simultaneously for the first three harmonics of the convected vortical gust of the rotor. The spatial mode generation, propagation and decay characteristics are predicted by assuming the acoustic field away from the stator can be represented as a uniform flow with small harmonic perturbations superimposed. The computed field is then decomposed using a joint temporal-spatial transform to determine the wave amplitudes as a function of rotor harmonic and spatial mode order. This report details the following technical aspects of the computations and analysis. 1) the BASS computational technique; 2) the application of periodic time shifted boundary conditions; 3) the linear theory aspects unique to rotor-stator interactions; and 4) the joint spatial-temporal transform. The computational results presented herein are twofold. In each case, the acoustic response of the stator is determined simultaneously for the first three harmonics of the convected vortical gust of the rotor. The fan under consideration here like modern fans is cut-off at +, and propagating acoustic waves are only expected at 2BPF and 3BPF. In the first case, the computations showed excellent agreement with linear theory predictions. The frequency and spatial mode order of acoustic field was computed and found consistent with linear theory. Further, the propagation of the generated modes was also correctly predicted. The upstream going waves propagated from the domain without reflection from the in ow boundary. However, reflections from the out ow boundary were noticed. The amplitude of the reflected wave was approximately 5% of the incident wave. The second set of computations were used to determine the influence of steady loading on the generated noise. Toward this end, the acoustic response was determined with three steady loading

  14. Reproducción natural controlada del black bass Micropterus salmoides - Controlled natural reproduction of black bass Micropterus salmoides

    Directory of Open Access Journals (Sweden)

    Amaral Junior, Hilton

    2009-09-01

    Full Text Available ResumenPara viabilizar la reproducción, larvicutura y alevinaje del Black bass, en la región sur de Brasil, se ha buscado adaptar una tecnología, desarrollada por el Centro Nacional de Acuicultura Piscifactoría las Vegas del Guadiana Badajoz/España. Consiste en controlar el ambiente de desove, utilizando estanques con nidales, para el desove del Black bass. Optimizando esta tecnología, desarrollamos una técnica de adaptación del Black bass al cultivo intensivo durante el período de invierno, ofreciendo un pienso de un 40% de proteína cruda y zooplancton (Daphnia sp.. Se dejan los reproductores en un mismo estanque de engorda en sistema extensivo, y son adaptados para un sistema intensivo de cultivo, en estanques de hormigón con entrada y salida del agua. Se acondicionan reproductores machos y hembras, durante los meses de invierno. En la primavera, se abren sucesivamente las compuertas de comunicación del departamentocentral, con los estanques frezaderos. En estos estanques frezaderos,se colocan nidales de grava para la puesta de las hembras. Pasado elperíodo de ocupación cíclica de los estanques de desove, losreproductores vuelven al departamento central. Después de laeclosión, las larvas son alimentadas con pienso molido a un 48% deproteína cruda con una oferta de un 10% de su peso y zooplancton Daphina sp. Los alevines se quedan alojados en estanques de tierrapara el período de engorda.SummaryIn order to make reproduction, larval and fingerling rearing of BlackBass in the south region of Brazil, we try to adapt a technologydeveloped by Centro Nacional de Aquicultura Piscifactoria Las Vegasdel Guadiana, Badajoz, Espanha. The technique consists in controllingthe spawning environment with a tank containing nests. To optimizethis technology, we develop a technique to adapt the black bass in aintensive culture during winter, providing food with 40% proteine andzooplankton (Daphnia sp.. The spawners are manteined in the

  15. Description of two new gill myxozoans from smallmouth (Micropterus dolomieu) and largemouth bass (Micropterus salmoides)

    Science.gov (United States)

    Walsh, Heather L.; Iwanowicz, Luke R.; Glenney, Gavin W.; Iwanowicz, Deborah D.; Blazer, Vicki

    2012-01-01

    Two previously undescribed species of myxozoan parasites were observed in the gills of bass inhabiting the Potomac and James River basins. They are described using morphological characteristics and small-subunit (SSU) rDNA gene sequences. Both were taxonomically identified as new species of Myxobolus; Myxobolus branchiarum n. sp. was found exclusively in smallmouth bass, and Myxobolus micropterii n. sp. was found in largemouth and smallmouth bass. Small, spherical, white plasmodia of M. branchiarum from smallmouth bass were observed grossly in the gills; these plasmodia had an average length of 320.3 µm and width of 246.1 µm. The development of the plasmodia is intralamellar in the secondary lamellae of the gills. Mature spores were pyriform in shape with a length of 12.8 ± 1.4 (8.1–15.1) µm and width of 6.9 ± 1.1 (4.0–9.0) µm. Analysis of SSU rDNA identified M. branchiarum in a sister-group to 3 species of Henneguya, although morphologically caudal appendages were absent. Myxobolus micropterii observed in the gills of largemouth and smallmouth bass had larger, ovoid, cream-colored plasmodia with an average length of 568.1 µm and width of 148.1 µm. The cysts developed at the distal end of the gill filament within the primary lamellae. The mature spores were ovoid in shape with a length of 10.8 ± 0.7 (9.2–12.2) µm and width of 10.6 ± 0.6 (9.0–11.8) µm. SSU rDNA analysis placed M. micropterii in a sister group with Henneguya lobosa and Myxobolus oliveirai. The highest prevalence of M. branchiarum was observed in the gills of bass collected from the Cowpasture River (50.9%). Prevalence was 44.6% in bass from the Potomac River and only 4.3% in bass collected from the Shenandoah River. A seasonal study of M. branchiarum, which included both infected and uninfected smallmouth bass, determined that a significantly higher intensity was observed in the spring than in the summer (P < 0.001) or fall (P  =  0.004). In an analysis excluding uninfected

  16. Geometric morphometric analysis of cyclical body shape changes in color pattern variants of Cichla temensis Humboldt, 1821 (Perciformes: Cichlidae) demonstrates reproductive energy allocation

    OpenAIRE

    Paul Reiss; Thomas M. Grothues

    2015-01-01

    Previously recognized color and pattern variants of adult Cichla temensis in Amazon flood pulse river environments reflect the cycling of individuals through seasonal sexual maturity and spawning. Individuals also vary in shape from blocky to fusiform. To determine if shape differences are related to patterns of fat reserve deposition and utilization, and to quantify the relationship of shape with color and pattern variation and life history status, specimens in each of four previously define...

  17. Why the Bass Model Fits without Decision Variables

    OpenAIRE

    Frank M. Bass; Trichy V. Krishnan; Dipak C. Jain

    1994-01-01

    Over a large number of new products and technological innovations, the Bass diffusion model (Bass 1969) describes the empirical adoption curve quite well. In this study, we generalize the Bass model to include decision variables such as price and advertising. The generalized model reduces to the Bass model as a special case and explains why the Bass model works so well without including decision variables. We compare our generalized Bass model to other approaches from the literature for inclu...

  18. Sound Generating Mechanism of the Double Bass

    Directory of Open Access Journals (Sweden)

    F. Pantelić

    2013-11-01

    Full Text Available This paper deals with the mechanical properties of the double bass and double bass bow. The sound of the instrument, as well as the vibrations of the bow, were recorded while playing. The sound was recorded with a microphone, while a sensor, placed on the bow’s stick, registered its vibrations. By observing these signals, from double bass and bow, the correlation between them was determined in order to explain the bow-string mechanism.

  19. 33 CFR 117.703 - Bass River.

    Science.gov (United States)

    2010-07-01

    ... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Bass River. 117.703 Section 117.703 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY BRIDGES DRAWBRIDGE OPERATION REGULATIONS Specific Requirements New Jersey § 117.703 Bass River. The draw of the U.S. 9...

  20. 33 CFR 117.588 - Bass River.

    Science.gov (United States)

    2010-07-01

    ... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Bass River. 117.588 Section 117.588 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY BRIDGES DRAWBRIDGE OPERATION REGULATIONS Specific Requirements Massachusetts § 117.588 Bass River. The Hall Whitaker...

  1. Spar genetic analysis of two invasive species of Cichla (Tucunaré (Perciformes: Cichlidae in the Paraná river basin = Análise genética via spar, de duas espécies de Cichla (Tucunaré (Perciformes: Cichlidae introduzidas na Bacia do rio Paraná

    Directory of Open Access Journals (Sweden)

    Giovanna Caputo Almeida-Ferreira

    2011-01-01

    Full Text Available The introduction of exotic species in lake ecosystems has been greatly highlighted in the literature worldwide. Since introduction may threaten diversity of native fish, the issue turns up to be of paramount importance. Ecological effects may be predation, competition, parasitism or genetic, that is, changes in the genetic pool of populations owing to the occurrence of hybrids. Although the Tucunaré fish (Cichla is native to the Amazon region, it can be foundin other hydrographic basins in which it has been introduced. RAPD molecular marker research showed that there are two species (Cichla kelberi and C. piquiti belonging to the genus Cichla in the rivers of the Paraná basin. Different morphotypes in the region may also be due to hybridization. Current research used SPAR molecular markers to confirm the presence of hybrids and the rupture of isolation mechanisms. Seventy-two specimens collected in several sites of the river Paraná and Amazon basins were analyzed. Since exclusive SPAR molecular markers were obtained for Cichla kelberi and C. piquiti populations, the introduction of the two species in the region has been confirmed. Identification of the markers in specimens of theParaná river basin confirmed hybridization between these exotic species.A introdução de espécies exóticas nos ecossistemas lacustres tem sido muito destacada na literatura mundial, pois ameaça a diversidade de peixes nativos, tornando-se uma questão de extrema importância. Os efeitos observados podem ser ecológicos, como predação, competição e parasitismo ou genéticos, como a ocorrência de híbridos. Apesar do peixe tucunaré (Cichla ser nativo da região amazônica, ele pode ser encontrado em outras bacias hidrográficas nas quais foram introduzidos. Estudos utilizando marcadores moleculares RAPD revelaram que existem duas espécies (Cichla kelberi e C. piquiti do gênero Cichla na bacia do alto rio Paraná e morfotipos diferentes que podem ser devido

  2. Spar genetic analysis of two invasive species of Cichla (Tucunaré) (Perciformes: Cichlidae) in the Paraná river basin = Análise genética via spar, de duas espécies de Cichla (Tucunaré) (Perciformes: Cichlidae) introduzidas na Bacia do rio Paraná

    OpenAIRE

    Giovanna Caputo Almeida-Ferreira; Alessandra Valéria de Oliveira; Alberto José Prioli; Sônia Maria Alves Pinto Prioli

    2011-01-01

    The introduction of exotic species in lake ecosystems has been greatly highlighted in the literature worldwide. Since introduction may threaten diversity of native fish, the issue turns up to be of paramount importance. Ecological effects may be predation, competition, parasitism or genetic, that is, changes in the genetic pool of populations owing to the occurrence of hybrids. Although the Tucunaré fish (Cichla) is native to the Amazon region, it can be foundin other hydrographic basins in w...

  3. Spar genetic analysis of two invasive species of Cichla (Tucunaré (Perciformes: Cichlidae in the Paraná river basin - doi: 10.4025/actascibiolsci.v33i1.4855 Spar genetic analysis of two invasive species of Cichla (Tucunaré (Perciformes: Cichlidae in the Paraná river basin - doi: 10.4025/actascibiolsci.v33i1.4855

    Directory of Open Access Journals (Sweden)

    Alberto José Prioli

    2011-02-01

    Full Text Available The introduction of exotic species in lake ecosystems has been greatly highlighted in the literature worldwide. Since introduction may threaten diversity of native fish, the issue turns up to be of paramount importance. Ecological effects may be predation, competition, parasitism or genetic, that is, changes in the genetic pool of populations owing to the occurrence of hybrids. Although the Tucunaré fish (Cichla is native to the Amazon region, it can be found in other hydrographic basins in which it has been introduced. RAPD molecular marker research showed that there are two species (Cichla kelberi and C. piquiti belonging to the genus Cichla in the rivers of the Paraná basin. Different morphotypes in the region may also be due to hybridization. Current research used SPAR molecular markers to confirm the presence of hybrids and the rupture of isolation mechanisms. Seventy-two specimens collected in several sites of the river Paraná and Amazon basins were analyzed. Since exclusive SPAR molecular markers were obtained for Cichla kelberi and C. piquiti populations, the introduction of the two species in the region has been confirmed. Identification of the markers in specimens of the Paraná river basin confirmed hybridization between these exotic species.The introduction of exotic species in lake ecosystems has been greatly highlighted in the literature worldwide. Since introduction may threaten diversity of native fish, the issue turns up to be of paramount importance. Ecological effects may be predation, competition, parasitism or genetic, that is, changes in the genetic pool of populations owing to the occurrence of hybrids. Although the Tucunaré fish (Cichla is native to the Amazon region, it can be found in other hydrographic basins in which it has been introduced. RAPD molecular marker research showed that there are two species (Cichla kelberi and C. piquiti belonging to the genus Cichla in the rivers of the Paraná basin. Different

  4. Diet of two species of fish family Cichlidae (Astronotus ocellatus and Cichla pinima) introduced in the Paraguaçu River, Bahia

    OpenAIRE

    Luis Rogério Godinho dos Reis; Alexandre Clistenes de Alcântara Santos

    2014-01-01

    Analysis of the diet of introduced fish species allows us to understand their trophic position in the community, their level of predation, and possibly to identify the if and when they act as competitors and/or predators. This paper aims to describe the diet of the tucunaré, Cichla pinima, and of the apanhari, Astronotus ocellatus. Both species originated from the Amazon basin and were introduced into the Paraguaçu River. Ninety-two specimens were analyzed, and both species showed a diversifi...

  5. Cage culture of sea bass in Malaysia

    OpenAIRE

    1994-01-01

    The article discusses the cage culture practice of sea bass (Lates calcarifer) in Malaysia. Problems on feed and seed supply and overcrowding are also discussed. Despite these problems, seabass cage culture still continuously booms.

  6. Bass Strait underpins Aussie oil flow

    International Nuclear Information System (INIS)

    This paper reports that the Bass Strait region off Australia's southern coast is expected to remain the mainstay of the country's oil production in the near term. Government and industry see Australia's western offshore areas as the key to maintaining the country's level of oil self-sufficiency at an acceptable level beyond 2000. But Bass Strait oil flow, in decline since 1985, at about 300,000 b/d still provides more than half of Australia's current production. Bass Strait operators have a healthy program of exploration and development on tap in the near term. Chief focus will be in the Gippsland basin, off Victoria's southeast coast, which currently provides most Bass Strait oil and gas production

  7. Intersex fish : Endocrine disruption in smallmouth bass

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — Intersex and abnormal vitellogenin in smallmouth bass from portions of the Potomac watershed pose a threat to fish resources. This fact sheet summarizes studies...

  8. Stock characteristics of Hudson River striped bass

    International Nuclear Information System (INIS)

    Striped bass, because of their tremendous popularity both commercially and recreationally, were a principal focus of the Hudson River power plant case. Between 1976 and 1979, over 23,000 age-II and older striped bass were studied as one facet of an extensive research program on the spring population in the Hudson River. Samples were collected from the overwintering as well as the spawning portion of the striped bass population, and included immature as well as mature fish. At least 12 age-groups contributed to spawning each year. Of these 12, age-groups III, IV, and V usually were most abundant, but the percentage of the population represented by any single age-group varied as the result of fluctuations in year-class strength. Males first became sexually mature at age II and females at age IV. Fast-growing individuals within a year class tended to mature earlier. Fecundity increased with the size of fish, reaching an observed maximum of about 3 million eggs per female. Although significant annual variations in maturity and growth were detected for Hudson River striped bass, there was no evidence of a consistent change in either variable that might be associated with increasing power plant operations and a reduction in striped bass abundance. Age at maturity and age structure are the two life history components that differ the most between the Hudson River population and other striped bass populations. 36 refs., 7 tabs

  9. Rumble in the Jungle: City, Place and Uncanny Bass

    OpenAIRE

    Chris Christodoulou

    2011-01-01

    While bass powerfully resonates among the cultural discourses, lexicology and commercial marketing of a range of electronic dance music (EDM) styles, little popular music scholarship has paid attention to the subjective, phenomenological and psycho-physiological significance of bass in its modulation of intense feelings of pleasure. This article examines the linking in jungle/drum 'n' bass culture of bass as a sonic space that produces a powerful sense of jouissance where identity can ...

  10. 76 FR 14804 - Drawbridge Operation Regulations; Bass River, Beverly, MA

    Science.gov (United States)

    2011-03-18

    ... SECURITY Coast Guard 33 CFR Part 117 Drawbridge Operation Regulations; Bass River, Beverly, MA AGENCY... the Hall Whitaker Bridge at mile 0.6 across the Bass River ] at Beverly, Massachusetts. The deviation.... SUPPLEMENTARY INFORMATION: The Hall Whitaker Bridge, across the Bass River at Beverly, Massachusetts, has...

  11. The fundamental thermal niche of adult landlocked striped bass

    Science.gov (United States)

    Bettoli, P.W.

    2005-01-01

    Researchers have described the temperatures selected by landlocked striped bass Morone saxatilis in different locales throughout the USA. However, seasonally low concentrations of dissolved oxygen (DO) in many systems prevented striped bass from using the cool waters (niche of adult landlocked striped bass may be lower than literature estimates. These results also represent the first unbiased field estimates of the influence of season on the thermal ecology of adult striped bass. The thermal characteristics of habitats considered optimal in habitat suitability index models for adult landlocked striped bass (i.e., 18-24??C) should be revised to include cooler waters. ?? Copyright by the American Fisheries Society 2005.

  12. Geometric morphometric analysis of cyclical body shape changes in color pattern variants of Cichla temensis Humboldt, 1821 (Perciformes: Cichlidae demonstrates reproductive energy allocation

    Directory of Open Access Journals (Sweden)

    Paul Reiss

    2015-03-01

    Full Text Available Previously recognized color and pattern variants of adult Cichla temensis in Amazon flood pulse river environments reflect the cycling of individuals through seasonal sexual maturity and spawning. Individuals also vary in shape from blocky to fusiform. To determine if shape differences are related to patterns of fat reserve deposition and utilization, and to quantify the relationship of shape with color and pattern variation and life history status, specimens in each of four previously defined grades of color and pattern variation were compared using geometric morphometric techniques. Progressive shape changes occurred between grades independent of sex and correlated to gonosomatic index (GSI. Thin plate spline deformation visualizations indicate that the observed shape differences are related to fat deposition patterns. The seasonal timing of shape change and its link to color pattern variation, sexual maturity and local water level conditions suggests a relationship between the physiological and behavioral characteristics of C. temensis and the cyclical flood pulse pattern of its habitat.

  13. Benzocaine as an anesthetic for striped bass

    Science.gov (United States)

    Gilderhus, Philip A.; Lemm, Carol A.; Woods, L. Curry, III

    1991-01-01

    Benzocaine was tested as an anesthetic on juvenile and mature adult striped bass (Morone saxatilis ). Concentrations of 55 mg/L at 22 degree C to 80 mg/L at 11 degree C effectively anesthetized fish in about 3 min. Recovery was more rapid as temperature increased. Fish survived concentrations of twice the effective concentration and exposure times up to 60 min at the effective concentration. Striped bass required higher concentrations for anesthetization than had been previously demonstrated for salmonid fishes, but safety margins for both concentration and exposure time were wider than for the salmonids.

  14. Book review: Black bass diversity: Multidisciplinary science for conservation

    Science.gov (United States)

    Jelks, Howard

    2016-01-01

    These proceedings are from the third symposium dedicated to management and conservation of black basses in the genus Micropterus. The first symposium was held in 1975 (R. H. Stroud and H. Clepper. Black Bass Biology and Management. Washington (DC): Sport Fishing Institute) followed 25 years later by Black Bass 2000 (D. P. Philipp and M. S. Ridgway. 2002. Black Bass: Ecology, Conservation, and Management. Bethesda (MD): American Fisheries Society). Although the previous books discussed conservation of genetic variation and distinct strains of basses, the bulk of the papers in those tomes emphasized management of largemouth and smallmouth bass. In contrast, this third symposium is focused on the rarer bass species and challenges for their successful management.

  15. Comparative Ecology of Juvenile Striped Bass and Juvenile Hybrid Striped Bass in Claytor Lake, Virginia

    OpenAIRE

    Rash, Jacob Michael

    2003-01-01

    Since the introduction of hybrid striped bass M. chrysops x M. saxatilis to Claytor Lake, Virginia in 1993, relative abundance of striped bass Morone saxatilis has dropped disproportionately to stocking density. Potentially deleterious interactions between the two fishes that may limit recruitment to age 1 were considered in terms of trophic relationships, physiological indices of health, overwinter survival, and post-stocking predation. Both fishes preferred habitat types characterized ...

  16. Spar genetic analysis of two invasive species of Cichla (Tucunaré) (Perciformes: Cichlidae) in the Paraná river basin - doi: 10.4025/actascibiolsci.v33i1.4855 Spar genetic analysis of two invasive species of Cichla (Tucunaré) (Perciformes: Cichlidae) in the Paraná river basin - doi: 10.4025/actascibiolsci.v33i1.4855

    OpenAIRE

    Alberto José Prioli; Sônia Maria Alves Pinto Prioli; Alessandra Valéria de Oliveira; Giovanna Caputo Almeida-Ferreira

    2011-01-01

    The introduction of exotic species in lake ecosystems has been greatly highlighted in the literature worldwide. Since introduction may threaten diversity of native fish, the issue turns up to be of paramount importance. Ecological effects may be predation, competition, parasitism or genetic, that is, changes in the genetic pool of populations owing to the occurrence of hybrids. Although the Tucunaré fish (Cichla) is native to the Amazon region, it can be found in other hydrographic basins in ...

  17. Effects of water velocity on activity of juvenile striped bass

    Energy Technology Data Exchange (ETDEWEB)

    Bowles, R.R.; Griffith, J.S.; Coutant, C.C.

    1976-07-01

    The swimming activity of juvenile striped bass (Morone saxatilis Walbaum) 8 to 80 mm long was investigated in a test chamber simulating, on a small scale, a fixed-screen cooling water intake structure. As water velocity increased from 0 to 30 cm/sec area and distance traveled by juvenile bass 10 to 80 mm long decreased. However, as water velocity increased from 0 to 3 cm/sec the area and distance covered by larval bass increased. The presence of food increased the activity of larval bass, but decreased the activity of juveniles. Area ranged by striped bass at test velocities ranging from 0 to 30 cm/sec increased in proportion to body length. Juvenile striped bass tested at acclimation temperatures between 20 and 5/sup 0/C experienced a 30% reduction of activity. Activity was also reduced as temperature increased from 20 to 30/sup 0/C.

  18. Spatial simulation of smallmouth bass in streams

    International Nuclear Information System (INIS)

    The hydropower industry and its regulators are hampered by the inability to predict the relationship between alternative flow regimes and fish population response. We have developed a spatially explicit, individual-based model of populations of small-mouth bass in streams as part of the Compensatory Mechanisms in Fish Populations Program (see Sale and Otto 1991). In the model, the profitability of alternative stream locations varies in response to habitat depth and velocity through changes in the frequency of prey encounters and the metabolic costs experienced by fish. We conducted an evaluation of our hydraulic simulation at the scale of individual stream cells. The potential error in predictions for individual cell velocities suggests that larger-scale model predictions for the representative reach are most appropriate. At this scale, the model appears to produce realistic patterns in the growth and dispersal of young-of-year small-mouth bass. This verification step allows us to proceed with greater confidence in evaluating the original question of how small-mouth bass populations respond to alternative flow regimes

  19. Diet of two species of fish family Cichlidae (Astronotus ocellatus and Cichla pinima introduced in the Paraguaçu River, Bahia

    Directory of Open Access Journals (Sweden)

    Luis Rogério Godinho dos Reis

    2014-12-01

    Full Text Available Analysis of the diet of introduced fish species allows us to understand their trophic position in the community, their level of predation, and possibly to identify the if and when they act as competitors and/or predators. This paper aims to describe the diet of the tucunaré, Cichla pinima, and of the apanhari, Astronotus ocellatus. Both species originated from the Amazon basin and were introduced into the Paraguaçu River. Ninety-two specimens were analyzed, and both species showed a diversified diet composed of 20 different food types. For C. pinima, fish (IAi = 35.2% Ephemeroptera insects (IAi = 31.7% and molluscs (IAi = 21.2% were the most important food types. Ephemeropteran insects were highly prevalent in the diet of A. ocellatus, corresponding to over 98% of IAi. The high prevalence of insects in the diet may be related to the high number of juveniles among the specimens analyzed. The presence of native fish in the stomach contents of tucunarés deserves particular attention, because the voracity of this fish can lead to extinction of native species.

  20. The importance of bass clarity in pop and rock venues

    DEFF Research Database (Denmark)

    Adelman-Larsen, Niels Werner; Thompson, Eric Robert

    2008-01-01

    High levels of bass sound have been shown to stimulate the part of the brain that controls such basic instincts as sexual desire and hunger [Todd, 2000]. In rock and pop music, the bass frequencies from 40-125 Hz get amplified to very loud levels. Easily half of the electrical power of the PA and...

  1. Rearing sunshine bass using diets formulated for summer water temperatures

    Science.gov (United States)

    Elevated water temperatures are common in hybrid striped bass or Sunshine bass (HSB; Morone chrysops x M. saxatilis) production ponds during summer months in the southern US. Median daily water temperatures often exceed 30 C from June through September. This experiment was conducted to extend and re...

  2. Philometra mirabilis sp. n. (Nematoda: Philometridae), a new gonad-infecting parasite from the freshwater fish Cichla mirianae (Cichlidae) in Brazilian Amazon

    Czech Academy of Sciences Publication Activity Database

    Moravec, František; Diggles, B.

    2015-01-01

    Roč. 114, č. 5 (2015), s. 1929-1932. ISSN 0932-0113 R&D Projects: GA ČR GBP505/12/G112 Institutional support: RVO:60077344 Keywords : parasitic nematode * Dracunculoidea * cichlid fish * ovary * Amazon River basin * Brazil Subject RIV: EG - Zoology Impact factor: 2.098, year: 2014

  3. 75 FR 38935 - Fisheries of the Northeastern United States; Black Sea Bass Fishery; 2010 Black Sea Bass...

    Science.gov (United States)

    2010-07-07

    ... emergency rule to increase the 2010 black sea bass specifications on February 10, 2010 (75 FR 6586). The... (75 FR 6586), NMFS requested, and subsequently received, comments on the increased black sea bass TAL... December 22, 2009 (74 FR 67978), and became effective on January 1, 2010. The final rule implemented a...

  4. THE DIATONIC ACCORDION BASS NOTES LEARNING APPLICATION

    OpenAIRE

    Koren, David

    2013-01-01

    Learning to play a musical instrument is usually tackle with the help of a music teacher. For learning purposes there already exist computer programs and applications that help the user in learning process. Many programs have a disadvantage of not showing the correct finger order while playing an instrument. In this thesis work we developed an application for an Apple iPad tablet, which teaches the user how to play the bass notes on the diatonic accordion. The presented information is based o...

  5. Isolation of TDA-producing Phaeobacter strains from sea bass larval rearing units and their probiotic effect against pathogenic Vibrio spp. in Artemia cultures

    DEFF Research Database (Denmark)

    Grotkjær, Torben; Bentzon-Tilia, Mikkel; D'Alvise, Paul;

    2016-01-01

    V. harveyi, which is the major bacterial pathogen in crustaceans and Mediterranean sea bass larvae cultures. Concomitantly, they significantly improved survival of V. harveyi-infected brine shrimp. 16S rRNA gene sequence homology identified the antagonists as Phaeobacter sp., and in silico DNA...

  6. A reservoir landscape for age-0 largemouth bass

    Science.gov (United States)

    Irwin, E.R.; Jackson, J.R.; Noble, R.L.

    2002-01-01

    Landscape ecology is concerned with how ecological processes are affected by spatial patterns. Identification of heterogeneity in littoral zones has expanded the conceptual framework of aquatic landscapes. Long-term study of a reservoir largemouth bass population indicated that the amount and arrangement of habitat regulated the population processes. The distribution of age-0 largemouth bass was quantified in relation to littoral habitat and relations between landscape features and population parameters on scales from embayment to microhabitat were determined. At the embayment scale, shoreline slope and amount of gravel substratum predicted fivefold variability in abundance among four reservoir embayments. Within an embayment, these habitat features explained between 37 and 88 percent of variation in shoreline distribution of age-0 largemouth bass. At the microhabitat scale, age-0 largemouth bass exhibited patchy distributions in relation to gravel substratum at 40 percent of sites. These results indicate that the landscape scale domain for young largemouth bass is large; whereas, specific patterns explained processes across multiple scales. Distributions of age-0 largemouth bass in relation to habitat, however, were apparent on a fine scale (10 m) and these data, coupled with limited movement behavior of young largemouth bass, indicate that the ecological neighborhood of this life stage is small. Our data also suggested that some habitats may be source habitats because embayments with hypothesized higher source/sink ratios were more productive. Although patch arrangement critical to young largemouth bass ecology was not quantified, it was apparent that embayments with more complex habitats likely provided the extent of the landscape for age-0 largemouth bass in Jordan Lake. Identification of scale of patchiness (of fish distributions and habitats) for this life stage will assist in making inferences regarding complex ecological processes that can affect year

  7. Reproducción natural controlada del black bass Micropterus salmoides - Controlled natural reproduction of black bass Micropterus salmoides

    OpenAIRE

    Amaral Junior, Hilton; Garcia, Silvano; Capozza Tebaldi, Paula

    2009-01-01

    ResumenPara viabilizar la reproducción, larvicutura y alevinaje del Black bass, en la región sur de Brasil, se ha buscado adaptar una tecnología, desarrollada por el Centro Nacional de Acuicultura Piscifactoría las Vegas del Guadiana Badajoz/España. Consiste en controlar el ambiente de desove, utilizando estanques con nidales, para el desove del Black bass. Optimizando esta tecnología, desarrollamos una técnica de adaptación del Black bass al cultivo intensivo durante el período de invierno, ...

  8. A Generalized Norton-Bass Model for Multigeneration Diffusion

    OpenAIRE

    Zhengrui Jiang; Dipak C. Jain

    2012-01-01

    The Norton-Bass (NB) model is often credited as the pioneering multigeneration diffusion model in marketing. However, as acknowledged by the authors, when counting the number of adopters who substitute an old product generation with a new generation, the NB model does not differentiate those who have already adopted the old generation from those who have not. In this study, we develop a generalized Norton-Bass (GNB) model that separates the two different types of substitutions. The GNB model ...

  9. NEW CAR DEMAND MODELING AND FORECASTING USING BASS DIFFUSION MODEL

    OpenAIRE

    Zuhaimy Ismail; Noratikah Abu

    2013-01-01

    Forecasting model of new product demand has been developed and applied to forecast new vehicle demand in Malaysia. Since the publication of the Bass model in 1969, innovation of new diffusion theory has sparked considerable research among marketing science scholars, operational researchers and mathematicians. The building of Bass diffusion model for forecasting new product within the Malaysian society is presented in this study. The proposed model represents the spread level of new Proton car...

  10. Rumble in the Jungle: City, Place and Uncanny Bass

    Directory of Open Access Journals (Sweden)

    Chris Christodoulou

    2011-06-01

    Full Text Available While bass powerfully resonates among the cultural discourses, lexicology and commercial marketing of a range of electronic dance music (EDM styles, little popular music scholarship has paid attention to the subjective, phenomenological and psycho-physiological significance of bass in its modulation of intense feelings of pleasure. This article examines the linking in jungle/drum 'n' bass culture of bass as a sonic space that produces a powerful sense of jouissance where identity can seem to unravel on the dance-floor and an articulation of contemporary urban space as a place of subjective loss and regression. Overlaying Freud's notion of the uncanny and Kristeva's signifying space of the chora, I discuss how this fetishisation of bass can be linked to the music's cultural formation from deindustrialised regions in London and the South-East of England during the early-1990s; its accelerated break-beats and 'dark' bass-lines can be seen to inscribe recent rapid social, cultural and environmental transformations in the urban metropolis.

  11. Assessment of endocrine disruption in Smallmouth bass (Micropterus dolomieu) and largemouth bass (Micropterus salmoides) in the Potomac River Watershed

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — Intersex, specifically testicular oocytes, has been observed in male smallmouth bass (SMB) and other centrarchids in the South Branch of the Potomac River and forks...

  12. 75 FR 59078 - Safety Zone; Ledge Removal Project, Bass Harbor, ME

    Science.gov (United States)

    2010-09-27

    ... SECURITY Coast Guard 33 CFR Part 165 RIN 1625-AA00 Safety Zone; Ledge Removal Project, Bass Harbor, ME... temporary safety zone around a ledge removal project in Bass Harbor, Maine. The United States Army Corps of... and around Bass Harbor both to increase mooring capacity for fishing trawlers and recreational...

  13. 77 FR 60904 - Safety Zone; Rio Vista Bass Derby Fireworks, Sacramento River, Rio Vista, CA

    Science.gov (United States)

    2012-10-05

    ... SECURITY Coast Guard 33 CFR Part 165 Safety Zone; Rio Vista Bass Derby Fireworks, Sacramento River, Rio... Guard will enforce the safety zone for the Rio Vista Bass Derby Fireworks in the Captain of the Port... 38 09'18'' N, 121 41'15'' W (NAD 83) for the Rio Vista Bass Derby Fireworks in 33 CFR 165.1191....

  14. Bass-SIR model for diffusion of new products

    CERN Document Server

    Fibich, Gadi

    2016-01-01

    We consider the diffusion of new products in social networks, where consumers who adopt the product can later "recover" and stop influencing others to adopt the product. We show that the diffusion is not described by the SIR model, but rather by a novel model, the Bass-SIR model, which combines the Bass model for diffusion of new products with the SIR model for epidemics. The phase transition of consumers from non-adopters to adopters is described by a non-standard Kolmogorov-Johnson-Mehl-Avrami model, in which clusters growth is limited by adopters' recovery. Therefore, diffusion in the Bass-SIR model only depends on the local structure of the social network, but not on the average distance between consumers. Consequently, unlike the SIR model, a small-worlds structure has a negligible effect on the diffusion. Surprisingly, diffusion on scale-free networks is nearly identical to that on Cartesian ones.

  15. V-moda bass freq低频耳塞

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    V-moda bass freq是一款为喜好低频的用户专门打造的耳塞.外观较为简洁。耳塞的主体单元部分采用了球状设计,表面采用了釉光处理,整体给人以珠圆玉润的感觉。由于专门为配合随声听而设计,所以bass freq非常易于驱动。

  16. Integration of Bass Enhancement and Active Noise Control System in Automobile Cabin

    Directory of Open Access Journals (Sweden)

    Liang Wang

    2008-01-01

    Full Text Available With the advancement of digital signal processing technologies, consumers are more concerned with the quality of multimedia entertainment in automobiles. In order to meet this demand, an audio enhancement system is needed to improve bass reproduction and cancel engine noise in the cabins. This paper presents an integrated active noise control system that is based on frequency-sampling filters to track and extract the bass information from the audio signal, and a multifrequency active noise equalizer to tune the low-frequency engine harmonics to enhance the bass reproduction. In the noise cancellation mode, a maximum of 3 dB bass enhancement can be achieved with significant noise suppression, while higher bass enhancement can be achieved in the bass enhance mode. The results show that the proposed system is effective for solving both the bass audio reproduction and the noise control problems in automobile cabins.

  17. VizieR Online Data Catalog: BANYAN. VII. Candidate YMG members from BASS (Gagne+, 2015)

    Science.gov (United States)

    Gagne, J.; Faherty, J. K.; Cruz, K. L.; Lafreniere, D.; Doyon, R.; Malo, L.; Burgasser, A. J.; Naud, M.-E.; Artigau, E.; Bouchard, S.; Gizis, J. E.; Albert, L.

    2015-09-01

    We obtained low-resolution NIR spectra of 241 candidate young moving group (YMG) members from the BANYAN All-Sky Survey (BASS), LP-BASS, and PRE-BASS samples. A description of individual observations is included in Table 1. There are three samples that are referred to in this Paper: (1) PRE-BASS consists of targets that were initially selected as potential members and followed up with spectroscopy, but that were later rejected as we modified our selection criteria to reject contaminants; (2) Low-Priority BASS (LP-BASS) consists of targets that have NIR colors only slightly redder than field dwarfs; and (3) BASS is the final sample presented in Paper V (Gagne et al., 2015, J/ApJ/798/73) that contains targets at least 1σ redder than field dwarfs and that has a lower fraction of contaminants. (6 data files).

  18. The ground-based solar observations database BASS 2000

    OpenAIRE

    Paletou, F; Lafon, M.; Maeght, P.; Grimaud, F.; Louge, T.; Aboudarham, J.

    2007-01-01

    BASS 2000 is the French solar database for ground-based instruments. We describe hereafter our organization, our tasks and the products we can deliver to the international community. Our prospects cover data mining into the THeMIS archive, a participation to the EST endeavour and the creation and curation of the ESPaDOnS/NARVAL stellar spectra database.

  19. Automatic Phrase Continuation from Guitar and Bass guitar Melodies

    DEFF Research Database (Denmark)

    Cherla, Srikanth; Purwins, Hendrik; Marchini, Marco

    2013-01-01

    A framework is proposed for generating interesting, and musically similar variations of a givenmonophonicmelody. The focus is on rock/pop guitar and bass-guitarmelodies with the aim of eventual extensions to other instruments and musical styles. It is demonstrated here how learning musical style...

  20. Raynaud´s phenomenon in a slap bass player

    DEFF Research Database (Denmark)

    Jepsen, Jørgen Riis; Simonsen, Jane Angel

    2016-01-01

    OBJECTIVE: Secondary Raynaud’s phenomenon is a frequent condition related to occupational exposure to local vibration but has not been described in musicians. This study aims to describe cold-induced blanching of the right second and (in particular) third digits in a 67-year-old double bass player...

  1. Topological Hochschild homology and the Bass trace conjecture

    DEFF Research Database (Denmark)

    Berrick, A. J.; Hesselholt, Lars

    2015-01-01

    We use the methods of topological Hochschild homology to shed new light on groups satisfying the Bass trace conjecture. Factorization of the Hattori–Stallings rank map through the Bökstedt–Hsiang–Madsen cyclotomic trace map leads to Linnell's restriction on such groups. As a new consequence of this...

  2. Results from BASS, the BANYAN All-Sky Survey

    Science.gov (United States)

    Gagne, Jonathan; Lafreniere, David; Doyon, Rene; Faherty, Jacqueline K.; Malo, Lison; Artigau, Etienne

    2015-01-01

    We present results from the BANYAN All-Sky Survey (BASS), a systematic all-sky survey for brown dwarf candidates in young moving groups. We describe a cross-match of the 2MASS and ALLWISE catalogs that provides a list of 98 970 potential nearby dwarfs with spectral types later than M5 with measurements of proper motion at precisions typically better than 15 masyr, as well as the Bayesian Analysis for Nearby Young AssociatioNs II tool (BANYAN II) which we use to build the BASS catalog from this 2MASS-ALLWISE cross-match, consisting of more than 300 candidate members of young moving groups. We present the first results of a spectroscopic follow-up of those candidates, which allowed us to identify several new low-mass stars and brown dwarfs displaying signs of low gravity. We use the BASS catalog to show tentative evidence for mass segregation in AB Doradus and Argus, and reveal a new ˜ 13 Mjup\\ co-moving companion to a young low-mass star in BASS. We obtain a moderate-resolution near-infrared spectrum for the companion, which reveals typical signs of youth and a spectral type L4γ.

  3. 75 FR 6586 - Fisheries of the Northeastern United; Black Sea Bass Fishery; 2010 Black Sea Bass Specifications...

    Science.gov (United States)

    2010-02-10

    ... black sea bass specifications was published in the Federal Register on December 22, 2009 (74 FR 67978...; 62 FR 44421) and finds the Council(s request meets both the criteria and justifications for invoking... 516 d at 74 FR 67978, Decembe r 22, 2009 Emergenc 4,500,000 2,041 800,000 363 3,700,000 1,678...

  4. Comparing catch orientation among Minnesota walleye, northern pike, and bass anglers

    Science.gov (United States)

    Schroeder, Susan A.; Fulton, David C.

    2013-01-01

    We compared the catch orientations of Minnesota walleye (Sander vitreus), northern pike (Esox lucius), largemouth bass (Micropterus salmoides), and smallmouth bass (Micropterus dolomieu) anglers. Results were derived from 2009, 2010, and 2012 surveys of anglers targeting these different species. Consistent with previous research, we identified four dimensions of anglers’ catch orientation: (a) catching something, (b) catching big fish, (c) catching many fish, and (d) keeping fish. Walleye anglers were the most motivated to keep fish, while northern pike anglers were more oriented toward catching big fish. Largemouth bass anglers, and to a lesser extent smallmouth bass anglers, were also oriented toward catching big fish. Bass anglers reported the lowest interest in keeping fish. An orientation to keep fish was negatively related to more restrictive management actions, regardless of species. A stronger orientation to catch big fish was associated with support for increased harvest restrictions only for northern pike and smallmouth bass.

  5. A comparison of mercury burdens between St. Marks National Wildlife Refuge and St. Andrew Bay, Florida: Evaluation of fish body burdens and physiological responses in largemouth bass, spotted seatrout, striped mullet, and sunfish

    Science.gov (United States)

    Huge, D.H.; Rauschenberger, R.H.; Wieser, F.M.; Hemming, J.M.

    2011-01-01

    Musculature from the dorsal region of 130 largemouth bass (Micropterus salmoides), 140 sunfish (Lepomis sp.), 41 spotted seatrout (Cynoscion nebulosus) and 67 striped mullet (Mugil cephalus) were collected from five estuarine and five freshwater sites within the St. Marks National Wildlife Refuge and two estuarine and two freshwater sites from St. Andrew Bay, Florida, United States of America. Musculature was analyzed for total mercury content, sagittal otoliths were removed for age determination and physiological responses were measured. Largemouth bass and sunfish from the refuge had higher mercury concentrations in musculature than those from the bay. Male spotted seatrout, male striped mullet, male and female sunfish and female largemouth bass had mercury burdens positively correlated with length. The majority of all four species of fish from both study areas contained mercury levels below 1.5 part per million, the limit for safe consumption recommended the Florida Department of Health. In comparison, a significant percentage of largemouth bass and sunfish from several sampled sites, most notably Otter Lake and Lake Renfroe within St. Marks National Wildlife Refuge, had mercury levels consistent with the health department's guidelines of 'limited consumption' or 'no consumption guidelines.'

  6. Organochlorines in cultivated sea bass and in diet

    Energy Technology Data Exchange (ETDEWEB)

    Gil, O.; Antunes, P. [Inst. Nacional de Investigacao Agraria e das Pescas/IPIMAR, Lisboa (Portugal)

    2004-09-15

    The main source of organochlorines bioaccumulation seems to be the food and marine species are considered one of the most important sources of organochlorines for human population. Therefore, it is essential to inventory the levels of these contaminants in marine organisms and find ways to reduce these levels. Aquaculture has been developed in the past decades and sea bass (Dicentrarchus labrax) is one of the main species produced in Portugal. The levels of organochlorines of commercial formulations of diets used in fish farming can influence the concentrations of fish produced in these systems of culture. In this study eighteen polychlorinated biphenyl (PCB) congeners and p,p'-DDT compounds were quantified in cultivated sea bass from two farms and the influence of size and diet contamination in bioaccumulation of these compounds was evaluated.

  7. Teaching Fourier Analysis and Wave Physics with the Bass Guitar

    CERN Document Server

    Courtney, M; Courtney, Michael; Althausen, Norm

    2006-01-01

    This article describes a laboratory or demonstration technique employing the bass guitar and a Vernier LabPro (or a PC soundcard) for teaching wave physics and introducing Fourier analysis. The Fourier transform of an open string provides a demonstration of oscillatory modes out to the 20th harmonic consistent with expectations containing a fundamental frequency and harmonics. The playing of "harmonics" (suppressing resonant modes by lightly touching the string to enforce nodes at desired locations) demonstrates oscillations made up (mostly) of individual modes. Students see that the complete set of Fourier components (fundamental and harmonics) present on the open string can be explicitly connected with individual resonant frequencies as described in typical textbook discussions of natural frequencies of waves on a string. The use of a bass guitar rather than the six string electric guitar allows higher harmonics to be individually excited, and it is also easier for students to play the harmonics themselves.

  8. C-BASS: The C-Band All Sky Survey

    Science.gov (United States)

    Pearson, Timothy J.; C-BASS collaboration

    2016-06-01

    The C-Band All Sky Survey (C-BASS) is a project to image the whole sky at a wavelength of 6 cm (frequency 5 GHz), measuring both the brightness and the polarization of the sky. Correlation polarimeters are mounted on two separate telescopes, one at the Owens Valley Observatory (OVRO) in California and another in South Africa, allowing C-BASS to map the whole sky. The OVRO instrument has completed observations for the northern part of the survey. We are working on final calibration of intensity and polarization. The southern instrument has recently started observations for the southern part of the survey from its site at Klerefontein near Carnarvon in South Africa. The principal aim of C-BASS is to allow the subtraction of polarized Galactic synchrotron emission from the data produced by CMB polarization experiments, such as WMAP, Planck, and dedicated B-mode polarization experiments. In addition it will contribute to studies of: (1) the local (corruption by Faraday rotation, and complements the full-sky maps from WMAP and Planck. I will present the project status, show results of component separation in selected sky regions, and describe the northern survey data products.C-BASS (http://www.astro.caltech.edu/cbass/) is a collaborative project between the Universities of Oxford and Manchester in the UK, the California Institute of Technology (supported by the National Science Foundation and NASA) in the USA, the Hartebeesthoek Radio Astronomy Observatory (supported by the Square Kilometre Array project) in South Africa, and the King Abdulaziz City for Science and Technology (KACST) in Saudi Arabia.

  9. Topological Hochschild homology and the Bass trace conjecture

    OpenAIRE

    Berrick, A. J.; Hesselholt, Lars

    2013-01-01

    We use the methods of topological Hochschild homology to shed new light on the groups satisfying the Bass trace conjecture. We show that the factorization of the Hattori-Stallings rank map through the Bokstedt-Hsiang-Madsen cyclotomic trace map leads to Linnell's restriction on such groups. As a new consequence of this restriction, we show that the conjecture holds for any group G with the property that every subgroup that is isomorphic to the additive group of rational numbers has nontrivial...

  10. The BASS survey for brown dwarfs in young moving groups

    Science.gov (United States)

    Gagne, Jonathan; Lafreniere, David; Doyon, Rene; Malo, Lison; Faherty, Jacqueline K.; Artigau, Etienne; Cruz, Kelle L.; Burgasser, Adam J.; Filippazzo, Joe; Naud, Marie-Eve; Albert, Loic; Bouchard, Sandie; Gizis, John; Robert, Jasmin; Nadeau, Daniel; Bowsher, Emily C.; Nicholls, Christine

    2016-01-01

    I will present in this dissertation talk the construction and follow-up of the BANYAN All-Sky Survey (BASS) that we led to identify dozens of new isolated young brown dwarfs in the Solar neighborhood, several of which have physical properties such as mass, age and temperature that make them similar to exoplanets that were recently discovered using the method of direct imaging.Such isolated analogs of the giant, gaseous exoplanets are precious benchmarks that will allow a deep characterization of their atmospheres using high-resolution and high signal-to-noise spectroscopy, which is made possible due to the absence of a nearby and bright host star.I will end by describing BASS-Ultracool, an extension of BASS that focuses on the identification of extremely cool isolated exoplanet analogs that display methane in their atmospheres. This survey has already uncovered the first bonafide T dwarf member of a moving group, the ~150 Myr AB Doradus T5, SDSS1110+0116.

  11. NEW CAR DEMAND MODELING AND FORECASTING USING BASS DIFFUSION MODEL

    Directory of Open Access Journals (Sweden)

    Zuhaimy Ismail

    2013-01-01

    Full Text Available Forecasting model of new product demand has been developed and applied to forecast new vehicle demand in Malaysia. Since the publication of the Bass model in 1969, innovation of new diffusion theory has sparked considerable research among marketing science scholars, operational researchers and mathematicians. The building of Bass diffusion model for forecasting new product within the Malaysian society is presented in this study. The proposed model represents the spread level of new Proton car among a given set of the society in terms of a simple mathematical function that elapsed since the introduction of the new car. With the limited amount of data available for the new car, a robust Bass model was developed to forecast the sales volume. A procedure of the proposed diffusion model was designed and the parameters were estimated. Results obtained by applying the proposed model and numerical calculation shows that the proposed diffusion model is robust and effective for forecasting demand of new Proton car. The proposed diffusion model is shown to forecast more effectively and accurately even with insufficient previous data on the new product.

  12. An Investigation into the Uptake of Contaminants in Largemouth Bass and Sediment from the Lower Tombigbee and Mobile Rivers, 1992

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This report summarizes a study with the following results: 1. Mercury was detected in all largemouth bass composite samples, with highest levels found in bass...

  13. High density culture of white bass X striped bass fingerlings in raceways using power plant heated effluent

    Energy Technology Data Exchange (ETDEWEB)

    Collins, C.M.; Burton, G.L.; Schweinforth, R.L.

    1983-06-01

    White bass (Morone chrysops) X striped bass (M. saxatilis) hybrids weighing 1691/lb were initially stocked in five 24 ft/sup 3/ floating screen cages for 20 days. Hybrids averaging one inch in total length and 361 fish/lb were released in four 614 ft/sup 3/ concrete raceways. Two stocking densities, 2.6 and 5.1 fish/ft/sup 3/, were evaluated in the 94-day study using a flow rate of 300 gpm/raceway. Water temperatures averaged 79/sup 0/F and water quality was adequate throughout the production period. Fish were hand fed to satiation daily. Columnaris and Aeromonas hydrophila caused the most serious disease problems. Gas supersaturation was suspect in high mortality levels during cage culture of hybrid bass fry. Cannibalism may have been responsible for unaccountable losses prior to raceway stocking and at harvest. The study yielded 5773 hybrids weighing 658 lb. The high density treatment showed greater weight gain, average weight, average length and percent survival as well as improved food conversion. Results suggest that higher stocking densities and periodic grading may increase production and suppress cannibalism. 10 references, 3 figures, 3 tables.

  14. Effect of salinity on expression of branchial ion transporters in striped bass (Morone saxatilis)

    DEFF Research Database (Denmark)

    Tipsmark, Christian Kølbaek; Madsen, Steffen Søndergaard; Borski, Russell John

    2004-01-01

    proteins in striped bass when compared with the less euryhaline brown trout. In both FW and SW, NEM-sensitive gill H+-ATPase activity was negligible in striped bass and approximately 10-fold higher in brown trout. This suggests that in striped bass Na+-uptake in FW may rely more on a relatively high...

  15. 76 FR 53831 - Fisheries of the Northeastern United States; Summer Flounder, Scup, and Black Sea Bass Fisheries...

    Science.gov (United States)

    2011-08-30

    ... summer flounder, scup, and black sea bass specifications published on December 28, 2010 (75 FR 81498). An... United States; Summer Flounder, Scup, and Black Sea Bass Fisheries; 2011 Summer Flounder, Scup, and Black Sea Bass Specifications; Correction AGENCY: National Marine Fisheries Service (NMFS), National...

  16. 77 FR 68723 - Fisheries of the Northeastern United States; Summer Flounder, Scup, and Black Sea Bass Fisheries...

    Science.gov (United States)

    2012-11-16

    ... Northeastern United States; Summer Flounder, Scup, and Black Sea Bass Fisheries; 2013-2014 Summer Flounder, 2013- 2014 Scup, and 2013 Black Sea Bass Specifications; 2013 Research Set- Aside Projects AGENCY... summer flounder, scup, and black sea bass fisheries, and the 2014 summer flounder and scup fisheries,...

  17. 77 FR 60945 - 2012-2013 Accountability Measure and Closure for Commercial Black Sea Bass in the South Atlantic

    Science.gov (United States)

    2012-10-05

    ... sector of black sea bass from June 1, 2012 to July 1, 2012 (77 FR 28305, May 14, 2012). The commercial... Measure and Closure for Commercial Black Sea Bass in the South Atlantic AGENCY: National Marine Fisheries... bass in the exclusive economic zone (EEZ) of the South Atlantic through this temporary final rule....

  18. Osmoregulatory effects of hypophysectomy and homologous prolactin replacement in hybrid striped bass

    DEFF Research Database (Denmark)

    Jackson, Leslie F; McCormick, Stephen D; Madsen, Steffen S;

    2005-01-01

    The effects of ovine prolactin (oPRL) and striped bass prolactin (sbPRL; Morone saxatilis) on plasma osmolality, electrolyte balance, and gill Na+,K+-ATPase activity were investigated in hypophysectomized (Hx), freshwater (FW)-acclimated, hybrid striped bass (M. saxatilis x Morone chrysops). They...

  19. Energy conversion efficiency of trout and sea bass production in the Black Sea, Turkey

    International Nuclear Information System (INIS)

    This study examined the current energy balance, energy conversion efficiency, and farm-level efficiency of trout and sea bass production in the Black Sea of Turkey. Using a structured survey, we collected data from nine monoculture trout farms and five polyculture trout and sea bass farms during the 2005-2006 production season. Energy values were calculated using energy equivalents of the inputs and outputs for the farms. Data envelopment analysis was used to calculate farm-level efficiency. The total energy use per cubic meter was 46.57 MJ for trout production and 87.13 MJ for sea bass production. The main energy inputs were feed and diesel oil for both trout and sea bass production. Indirect energy use was dominant in trout production, while direct energy use was more common in sea bass production. The energy input-to-output ratio of trout production was higher than that of sea bass production. Sea bass production was more economically energy efficient compared to trout production. The allocative and economic energy efficiencies for trout and sea bass production were 0.788 and 0.881, respectively. For both types of farms, the most discriminative variable affecting economic efficiency was operator experience. Increasing focus on product distribution costs could decrease total energy costs by approximately 12%

  20. 77 FR 65136 - Fisheries of the Northeastern United States; Black Sea Bass Fishery; Recreational Quota Harvested

    Science.gov (United States)

    2012-10-25

    ... the 2012 fishing year is 1.86 million lb (844 mt) (76 FR 82189, December 30, 2011). The 2012 RHL was... Northeastern United States; Black Sea Bass Fishery; Recreational Quota Harvested AGENCY: National Marine...: Temporary rule; closure. SUMMARY: NMFS announces that the 2012 black sea bass recreational harvest limit...

  1. Effect of temperature on larval sunshine bass growth and survival to the fingerling stage

    Science.gov (United States)

    Determining the optimum conditions for tank culture of sunshine bass fingerlings will facilitate a year-round supply of seed for the production cycle of this increasingly popular food fish. This experiment determined the relationship between temperature and larval sunshine bass growth and survival ...

  2. Reproductive cycles of largemouth bass (Micropterus salmoides) in a cooling reservoir

    International Nuclear Information System (INIS)

    Annual reproductive cycles of largemouth bass (Micropterus salmoides) collected in the heated area of a 1120-hectare reservoir receiving thermal effluent from the Savannah River Plant were similar to cycles from bass collected in unheated waters during 1969 and 1970. Average maximum monthly temperatures at the heated area exceeded those in unheated waters by greater than 100C for the 2 years. Few monthly differences in gonosomatic indices were found between heated and unheated areas. Earlier attainment of maximum gonadal size and the presence of significantly larger juvenile bass at the heated area suggested that reproduction might be accelerated by thermal discharge. However, gonadal condition indicated that the reproductive period started in March and continued through April in both areas. Reproduction may have been advanced in some heated-area bass, although this was not obvious from overall changes in the reproductive cycles of bass from unheated areas. (auth)

  3. Gonadotropins in European sea bass: Endocrine roles and biotechnological applications.

    Science.gov (United States)

    Mazón, María José; Molés, Gregorio; Rocha, Ana; Crespo, Berta; Lan-Chow-Wing, Olivier; Espigares, Felipe; Muñoz, Iciar; Felip, Alicia; Carrillo, Manuel; Zanuy, Silvia; Gómez, Ana

    2015-09-15

    Follicle stimulating hormone (Fsh) and luteinizing hormone (Lh) are central endocrine regulators of the gonadal function in vertebrates. They act through specific receptors located in certain cell types found in the gonads. In fish, the differential roles of these hormones are being progressively elucidated due to the development of suitable tools for their study. In European sea bass (Dicentrarchus labrax), isolation of the genes coding for the gonadotropin subunits and receptors allowed in first instance to conduct expression studies. Later, to overcome the limitation of using native hormones, recombinant dimeric gonadotropins, which show different functional characteristics depending on the cell system and DNA construct, were generated. In addition, single gonadotropin beta-subunits have been produced and used as antigens for antibody production. This approach has allowed the development of detection methods for native gonadotropins, with European sea bass being one of the few species where both gonadotropins can be detected in their native form. By administering recombinant gonadotropins to gonad tissues in vitro, we were able to study their effects on steroidogenesis and intracellular pathways. Their administration in vivo has also been tested for use in basic studies and as a biotechnological approach for hormone therapy and assisted reproduction strategies. In addition to the production of recombinant hormones, gene-based therapies using somatic gene transfer have been offered as an alternative. This approach has been tested in sea bass for gonadotropin delivery in vivo. The hormones produced by the genes injected were functional and have allowed studies on the action of gonadotropins in spermatogenesis. PMID:26002037

  4. Alternative plant protein sources in sea bass diets

    Directory of Open Access Journals (Sweden)

    Edo D’Agaro

    2010-01-01

    Full Text Available A control diet (C containing animal protein (mainly fish meal was compared with 6 experimental diets containing different  plant proteins (soybean meal, SM; rapeseed meal, RM; potato protein concentrate, PPC and a mix of the three vegetable  protein sources, M. The plant protein replaced either 25 (1 or 50 (2% of the animal protein with the exception of diet  RM2 where the substitution rate was lowered to 35%, and in diet M where 55% of the total protein given was replaced in  equal amounts by the three plant proteins. For the growth trial, which lasted 97 days, 528 European sea bass (initial live  weight 107 ± 0,06g, distributed among 24 fibreglass tanks with three replicates per treatment, were used. The pelleted  feed was distributed 5 times per day using an automatic dispenser. Energy, crude protein and crude fat digestibility values  for fish meal and soybean meal were similar and not statistically different while the values for rapeseed meal and potato  protein concentrate were significantly lower. Digestive utilization for NFE was higher in fish meal and decreased significant-  ly in soybean meal, rapeseed meal and even more noticeably in potato protein concentrate. Diet digestibility values showed  a similar trend with a clear worsening effect at the higher inclusion rates used. Diet M gave digestibility coefficients lower  than those observed with diets C, SM1, SM2, RS1and RS2and higher than those of diets PPC1and PPC2. Fish fed a diet in  which 25% of the total protein was replaced by soybean had similar performances to those of the control group. On the  other hand, sea bass fed diets SM2, RS1, RS2and M had lower growth rates and worse feed utilization than those observed  with the control. Finally, specific growth rates and food conversion efficiency in sea bass fed diets containing potato protein  concentrate were poor because of the low palatability. These results show that soybean meal can substitute up to 25% of

  5. Four types of neoplasms in Asian sea bass (Lates calcarifer)

    Institute of Scientific and Technical Information of China (English)

    Ramalingam Vijayakumar; Kuzhanthaivel Raja; Vijayapoopathi Singaravel; Ayyaru Gopalakrishnan

    2015-01-01

    Objective:To describe and observe four types of neoplasms on different parts (external and internal organs) of an Asian sea bass (Lates calcarifer). Methods:The sample was collected from local fish landing center (south east coast of India). Histopathology of normal and tumour tissues were analyzed. Results:A total of 83 tumour masses (neoplasm) were recorded on the fish skin, also the neoplasms were recorded in internal organs of fish such as liver, stomach and ovary. Conclusions:Aetiology of such neoplasm’s are unknown, further more researches need to confirm the causative agent for this type of neoplasm.

  6. Permanent genetic resources added to molecular ecology resources database 1 April 2012 - 31 May 2012.

    Science.gov (United States)

    Abelló, P; Ai, Weiming; Altmann, Carolin; Bernardi, Giacomo; Bonato, Olivier; Burchhardt, Kathleen M; Chen, Xiao; Chen, Zhijian; Cížková, Dagmar; Clouet, Cécile; Cubeta, Marc A; Garcia-Merchan, V H; Gauthier, Nathalie; Gibson, Shane; Halačka, Karel; Hamdi, Faten; Hankeln, Thomas; Hochkirch, Axel; Hrbek, Tomas; Jackson, Alexis M; Lin, Chongwen; Lin, Si-Min; Macpherson, E; Macrander, Jason; Marešová, Eva; Mendel, Jan; Nowak, Michał; Orti, Guillermo; Palero, F; Papoušek, Ivo; Pascual, M; Schmitt, Thomas; Semmens, Brice X; Streito, Jean-Claude; Tian, En-Wei; Tseng, Shu-Ping; Veith, Michael; Vetešník, Lukáš; Wang, Hurng-Yi; Weyer, Jessica; Willis, Stuart; Yu, Hui; Zhou, Zhiming

    2012-09-01

    This article documents the addition of 123 microsatellite marker loci to the Molecular Ecology Resources Database. Loci were developed for the following species: Brenthis ino, Cichla orinocensis, Cichla temensis, Epinephelus striatus, Gobio gobio, Liocarcinus depurator, Macrolophus pygmaeus, Monilinia vaccinii-corymbosi, Pelochelys cantorii, Philotrypesis josephi, Romanogobio vladykovi, Takydromus luyeanus and Takydromus viridipunctatus. These loci were cross-tested on the following species: Cichla intermedia, Cichla ocellaris, Cichla pinima, Epinephelus acanthistius, Gobio carpathicus, Gobio obtusirostris, Gobio sp. 1, Gobio volgensis, Macrolophus costalis, Macrolophus melanotoma, Macrolophus pygmaeus, Romanogobio albipinnatus, Romanogobio banaticus, Romanogobio belingi, Romanogobio kesslerii, Romanogobio parvus, Romanogobio pentatrichus, Romanogobio uranoscopus, Takydromus formosanus, Takydromus hsuehshanesis and Takydromus stejnegeri. PMID:22898144

  7. Testing the thermal-niche oxygen-squeeze hypothesis for estuarine striped bass

    Science.gov (United States)

    Kraus, Richard T.; Secor, D.H.; Wingate, Rebecca L.

    2015-01-01

    In many stratified coastal ecosystems, conceptual and bioenergetics models predict seasonal reduction in quality and quantity of fish habitat due to high temperatures and hypoxia. We tested these predictions using acoustic telemetry of 2 to 4 kg striped bass (Morone saxatilis Walbaum) and high-resolution spatial water quality sampling in the Patuxent River, a sub-estuary of the Chesapeake Bay, during 2008 and 2009. Striped bass avoided hypoxic (dissolved oxygen ≤2 mg·l−1) subpycnocline waters, but frequently occupied habitats with high temperatures (>25 °C) in the summer months, as cooler habitats were typically not available. Using traditional concepts of the seasonal thermal-niche oxygen-squeeze, most of the Patuxent estuary would beconsidered unsuitable habitat for adult striped bass during summer. Application of a bioenergetics model revealed that habitats selected by striped bass during summer would support positive growth rates assuming fish could feed at one-half ofmaximum consumption. Occupancy of the estuary during summer by striped bass in this study was likely facilitated by sufficient prey and innate tolerance of high temperatures by sub-adult fish of the size range that we tagged. Our results help extend the thermalniche oxygen-squeeze hypothesis to native populations of striped bass in semi-enclosed coastal systems. Tolerance of for supraoptimal temperatures in our study supports recent suggestions by others that the thermal-niche concept for striped bass should be revised to include warmer temperatures.

  8. Striped Bass Spawning in Non-Estuarine Portions of the Savannah River

    International Nuclear Information System (INIS)

    Historically, the estuarine portions of the Savannah River have been considered to be the only portion of the river in which significant amounts of striped bass (Morone saxatilis) spawning normally occur. A reexamination of data from 1983 through 1985 shows a region between River Kilometers 144 and 253 where significant numbers of striped bass eggs and larvae occur with estimated total egg production near that currently produced in the estuarine reaches. It appears possible that there are two separate spawning populations of striped bass in the Savannah River.

  9. Striped Bass Spawning in Non-Estuarine Portions of the Savannah River

    Energy Technology Data Exchange (ETDEWEB)

    Martin, D.; Paller, M.

    2007-04-17

    Historically, the estuarine portions of the Savannah River have been considered to be the only portion of the river in which significant amounts of striped bass (Morone saxatilis) spawning normally occur. A reexamination of data from 1983 through 1985 shows a region between River Kilometers 144 and 253 where significant numbers of striped bass eggs and larvae occur with estimated total egg production near that currently produced in the estuarine reaches. It appears possible that there are two separate spawning populations of striped bass in the Savannah River.

  10. Otolith Sr concentration analyzed by PIXE in Ariake estuary-dependent sea bass juveniles

    International Nuclear Information System (INIS)

    Japanese sea bass (Lateolabrax japonicus) is a typical euryhaline marine fish and frequently migrates from salt to freshwater environments during early life stages. We hypothesized that strontium concentrations in the otolith could be a useful index to examine freshwater entry because of its lower concentration in freshwater. Otoliths of Japanese sea bass juveniles collected in the Chikugo river and estuary were analyzed by Particle Induced X-ray Emission (PIXE) to see relationship between strontium concentration and ambient salinity. Strontium concentrations in otoliths of sea bass juveniles are significantly lower in the river samples than in brackish water samples. (author)

  11. Mercury in largemouth bass and spotted gar of the Florida Panther National Wildlife Refuge

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — From June 21 to 25, 1990, 21 largemouth bass (Micropterussa salmoides) and five spotted gar (Lepisosteus oculatus) were collected from selected locations at the...

  12. Contaminants in striped bass from the flint and Apalachicola Rivers 1986-1989

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — Five striped bass (Morone saxitilis), collected between April 1986 and May 1989 from the Flint River at Albany, Georgia, and the Apalachicola River at...

  13. Siim Nestor soovitab : Back2Bass Helsinki. Teenage Kicks / Siim Nestor

    Index Scriptorium Estoniae

    Nestor, Siim, 1974-

    2002-01-01

    Soome trummi-ja-bassi klubi Back2Bass 28. septembril KuKu klubis Tallinnas. 28. sept. Von Krahlis toimuvast live-üritusest Teenage Kicks, kus ansambel Claire's Birthaday esitleb ka oma uut singlit "Do You Remember"

  14. Mercury concentrations in largemouth bass and other fishes of the Loxahatchee National Wildlife Refuge

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — From September 17-21, 1990, 32 largemouth bass (Micropterus salmoides) and three other fish species were collected from eight locations at the Loxahatchee National...

  15. Mercury concentrations in largemouth bass of the Crystal River National Wildlife Refuge

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — From July 17 to 26, 1990, 20 largemouth bass (Micropterus salmoides) were collected from four locations at the Crystal River National Wildlife Refuge, Citrus...

  16. Mercury in largemouth bass of the Lake Woodruff National Wildlife Refuge

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — From August 24 to 28, 1990, largemouth bass (Micropterus salmoides) were collected from selected locations at the Lake Woodruff National Wildlife Refuge, Volusia...

  17. Mercury in largemouth bass and other fishes of the Merritt Island National Wildlife Refuge

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — From August 16 to 22, 1990, largemouth bass (Micropterus salmoides), bowfin (Amia calva) and spotted seatrout (Cynoscion nebulosus) were collected from selected...

  18. The effect of oceanography on sedimentology and geochemistry of the temperate carbonates of Bass Strait, Australia

    International Nuclear Information System (INIS)

    Full text: Modern cool temperate carbonates occur on the shallow shelf (50 -70 m) of' Bass Strait in an area of approximately 85,000 Km2 between latitudes of 38 deg to 40.5 deg S and longitudes of about 143 deg.30' to 149 deg F. Bass Strait carbonates are mainly affected by different water masses that consist of the warm saline Leeuwin Current and low salinity cold sub-Antarctic water, to the west, while to the east a weak intrusion of high salinity, warm, East Australian Current and relatively low salinity, cool, Tasman Sea water. To recognise the physical and chemical effect of these water masses on the sediments of the region, samples from eastern and western Bass Strait have been selected. The gravel size fractions are mostly distributed in the shallow shelf areas surrounding the islands. The higher gravel concentration nears the islands is attributed to the input of terrigenous materials from these islands and also the occurrence of large skeletal fragments such as molluscs. The sand size fractions are distributed throughout the area due to changes in water energy in different parts of the shelf. In Bass Strait a combination of bryozoans, molluscs and to some extent foraminifera, comprise the main components of the bulk sediments. The proportion of bryozoans is higher in the eastern rather than western Bass Strait. This is due to the more stable oceanographic conditions to the east, where the water energy is less, temperature and salinity are more uniform and the water contains higher concentration of nutrients. The carbonate mineralogy in Bass Strait is influenced by seawater temperature, and this is influenced by the water currents rather than by changes in depth or latitude. The Ca and Mg contour maps correlate well in the eastern and western of Bass Strait, due to the formation of higher amounts of high-Mg calcite in these areas. The Sr concentration is mainly related to carbonate mineralogy. In eastern Bass Strait, the relatively high Sr content is

  19. Ocean acidification impacts on black sea bass and scup embryos, responses of finfish in laboratory experiments

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Black sea bass (Centropristis striata) and scup (Stenotomus chrysops) compose important recreational and commercial fisheries along the United States Atlantic...

  20. Biometry traits and geometric morphometrics in sea bass (Dicentrarchus labrax) from different farming systems

    OpenAIRE

    Emilio Tibaldi; Maria Messina; Ivana Balenovic; Francesca Tulli

    2010-01-01

    The effect of the farming system on biometry traits and dressing out yield were inves- tigated in market-size European sea bass (Dicentrarchus labrax) cultured extensively or intensively in sea cages or land-based basins. Fish external appearences and shapes were studies with geometric morphometrics in order to assess the potential of combined methodologies in the assessment of finfish quality. Both standard biometry and geometric morphometrics were able to discriminate between sea bass farme...

  1. Feeding activity and spawning time of striped bass in the Colorado River Inlet, Lake Powell, Utah

    Science.gov (United States)

    Persons, William R.; Bulkly, R.V.

    1982-01-01

    Striped bass, Morone saxatilis, from Lake Powell, Utah spawned in or near the mixing zone of the reservoir and the Colorado River in 1980 and 1981. The fish did not move through Cataract Canyon rapids just above the reservoir in either year. Of 321 adult striped bass stomachs examined, 30% contained food and 28% contained threadfin shad, Dorosoma petenense. No stomachs contained native threatened or endangered Colorado River fishes.

  2. Appearent Digestibility of Plant Protein Based Diets by European Sea Bass Dicentrarchus labrax L., 1758

    OpenAIRE

    Altan, Özgür; Ali Yıldırım Korkut

    2011-01-01

    Commercial culture of carnivorous fish demands the reduction of environmental impact of feeds; that requires minimal use of dietary animal protein. This study investigated the digestibility of diets formulated exclusively out of plant protein, added rendered ingredients and feed attractants, by the carnivore European sea bass, Dicentrarchus labrax. Juvenile European sea bass (14.0±1.0 cm) conditioned to accept artificial dry feed were stocked in polypropylene cages and fed a...

  3. Massive Microbiological Groundwater Contamination Associated with a Waterborne Outbreak in Lake Erie, South Bass Island, Ohio

    OpenAIRE

    Fong, Theng-Theng; Mansfield, Linda S.; Wilson, David L.; Schwab, David J.; Molloy, Stephanie L.; Rose, Joan B.

    2007-01-01

    Background A groundwater-associated outbreak affected approximately 1,450 residents and visitors of South Bass Island, Ohio, between July and September 2004. Objectives To examine the microbiological quality of groundwater wells located on South Bass Island, we sampled 16 wells that provide potable water to public water systems 15–21 September 2004. Methods We tested groundwater wells for fecal indicators, enteric viruses and bacteria, and protozoa (Cryptosporidium and Giardia). The hydrodyna...

  4. Die faktorstruktuur van Bass se veelfaktor- leierskapsvraelys in die Suid-Afrikaanse konteks

    OpenAIRE

    C .P. Ackermann; J. M. Schepers; B. C. Lessing; Z. Dannhauser

    2000-01-01

    The factor structure of Bass's Multifactor Leadership Questionnaire in the South African context. The aim of the study was to determine whether the factor structure of Bass's Multifactor Leadership Questionnaire (MLQ), as a measure of transformational leadership, could be replicated within the South African context. The MLQ was chosen not only because it promised to be a valid and reliable measuring instrument of the construct in question, but also due to the fact that there was an urgent nee...

  5. Experimental Validation of Adjusting the Resonances of a Simplified Bass Clarinet Through Modal Active Control

    OpenAIRE

    Meurisse, Thibaut; MAMOU-MANI, Adrien; Benacchio, Simon; Finel, Victor; Chomette, Baptiste; Causse, René; SHARP, David

    2014-01-01

    This paper reports the experimental results of modifying the resonances of wind instruments using modal active control. Resonances of a simplified bass clarinet (a cylindrical tube coupled to a bass clarinet mouthpiece including a reed) are adjusted either in frequency or in damping in order to modify its playing properties (pitch, strength of the harmonics of the sound, transient behavior). This is achieved using a control system made up of a collocated loudspeaker and microphone linked by a...

  6. A STUDY ON NEW PRODUCT DEMAND FORECASTING BASED ON BASS DIFFUSION MODEL

    OpenAIRE

    Zuhaimy Ismail; Noratikah Abu

    2013-01-01

    A forecasting model of new product demand has been developed and applied to forecast new vehicle demand in Malaysia. Since the publication of the Bass model in 1969, innovation of new diffusion theory has sparked considerable research among marketing science scholars, operational researchers and mathematicians. This study considers the Bass Model for forecasting the diffusion of new products or an innovation in the Malaysian society. The objective of the proposed model is to represent the lev...

  7. A Generalized Bass Model for Product Growth in Networks

    CERN Document Server

    Manshadi, Vahideh H

    2016-01-01

    Many products and innovations become well-known and widely adopted through the social interactions of individuals in a population. The Bass diffusion model has been widely used to model the temporal evolution of adoption in such social systems. In the model, the likelihood of a new adoption is proportional to the number of previous adopters, implicitly assuming a global (or homogeneous) interaction among all individuals in the network. Such global interactions do not exist in many large social networks, however. Instead, individuals typically interact with a small part of the larger population. To quantify the growth rate (or equivalently the adoption timing) in networks with limited interactions, we study a stochastic adoption process where the likelihood that each individual adopts is proportional to the number of adopters among the small group of persons he/she interacts with (and not the entire population of adopters). When the underlying network of interactions is a random $k$-regular graph, we compute t...

  8. Contribution à la conception de circuits intégrés analogiques en technologie CMOS basse tension pour application aux instruments d'observation de la Terre

    OpenAIRE

    Standarovski, Denis

    2005-01-01

    Le présent mémoire de thèse s'inscrit dans la problématique d'intégration de chaînes pour traitement du signal vidéo issu d'un capteur CCD dédiées aux instruments d'observation de la Terre. La solution présentée à travers cette étude consiste à concevoir des circuits intégrés spécifiques (ASIC) analogiques avec des technologies CMOS sub-microniques basse-tension, principalement développées pour les circuits numériques complexes. Dans une première partie, nous présentons le contexte de l'étude...

  9. Behavioural thermoregulation and bioenergetics of riverine smallmouth bass associated with ambient cold-period thermal refuge

    Science.gov (United States)

    Westhoff, J.T.; Paukert, Craig P.; Ettinger-Dietzel, Sarah; Dodd, H.R.; Siepker, Michael

    2016-01-01

    Smallmouth bass in thermally heterogeneous streams may behaviourally thermoregulate during the cold period (i.e., groundwater temperature greater than river water temperature) by inhabiting warm areas in the stream that result from high groundwater influence or springs. Our objectives were to determine movement of smallmouth bass (Micropterus dolomieu) that use thermal refuge and project differences in growth and consumption among smallmouth bass exhibiting different thermal-use patterns. We implanted radio transmitters in 29 smallmouth bass captured in Alley Spring on the Jacks Fork River, Missouri, USA, during the winter of 2012. Additionally, temperature archival tags were implanted in a subset of nine fish. Fish were tracked using radio telemetry monthly from January 2012 through January of 2013. The greatest upstream movement was 42.5 km, and the greatest downstream movement was 22.2 km. Most radio tagged fish (69%) departed Alley Spring when daily maximum river water temperature first exceeded that of the spring (14 °C) and during increased river discharge. Bioenergetic modelling predicted that a 350 g migrating smallmouth bass that used cold-period thermal refuge would grow 16% slower at the same consumption level as a fish that did not seek thermal refuge. Contrary to the bioenergetics models, extrapolation of growth scope results suggested migrating fish grow 29% more than fish using areas of stream with little groundwater influence. Our results contradict previous findings that smallmouth bass are relatively sedentary, provide information about potential cues for migratory behaviour, and give insight to managers regarding use and growth of smallmouth bass in thermally heterogeneous river systems.

  10. A STUDY ON NEW PRODUCT DEMAND FORECASTING BASED ON BASS DIFFUSION MODEL

    Directory of Open Access Journals (Sweden)

    Zuhaimy Ismail

    2013-01-01

    Full Text Available A forecasting model of new product demand has been developed and applied to forecast new vehicle demand in Malaysia. Since the publication of the Bass model in 1969, innovation of new diffusion theory has sparked considerable research among marketing science scholars, operational researchers and mathematicians. This study considers the Bass Model for forecasting the diffusion of new products or an innovation in the Malaysian society. The objective of the proposed model is to represent the level of spread on new products among a given set of society in terms of a simple mathematical function that elapsed since the introduction of new products. With limited amount of data available for new products, a robust Bass model was developed to forecast the sales volume. A procedure of the proposed diffusion model was designed and the parameters were estimated. Results obtained by applying the proposed model and numerical calculation show that the proposed Bass diffusion model is robust and effective for forecasting demand of new products. This study concludes that the newly developed bass diffusion of demand function has significantly contributed for forecasting the diffusion of new products.

  11. Dispersal of smallmouth bass from a simulated tournament weigh-in site

    Science.gov (United States)

    Kaintz, Melissa A.; Bettoli, Phillip William

    2010-01-01

    Simulated smallmouth bass Micropterus dolomieu fishing tournaments were staged in Dale Hollow Lake, a 12,400-ha reservoir in Tennessee, between March 2004 and February 2005 to investigate posttournament dispersal. Smallmouth bass (n = 54) were captured with conventional hook-and-line tackle and artificial lures, placed in live wells, and subjected to a weigh-in procedure before being externally tagged with an ultrasonic transmitter and released. Water temperatures ranged from 7.4°C to 29.3°C (mean [SE] = 17.6°C [2.5]), fish ranged in total length from 330 to 572 mm (mean = 452 [8.3]), and no fish were dead at the weigh-ins. Smallmouth bass dispersed rapidly away from the release site, which was located at the head of a 68-ha embayment. After 3-5d, survivors (n = 44) traversed an average distance of 1,475 m [213]. Most (72%) fish swam uplake and out of the 385-ha study area after 6 d. The rapid dispersal of smallmouth bass may be relevant in systems that experience heavy tournament activity. The smallmouth bass caught and subjected to simulated tournament conditions on Dale Hollow Lake did not stockpile near the release site.

  12. 76 FR 82189 - Fisheries of the Northeastern United States; Summer Flounder, Scup, and Black Sea Bass Fisheries...

    Science.gov (United States)

    2011-12-30

    ... deduction. Consistent with the revised quota setting procedures for the FMP (67 FR 6877, February 14, 2002... Northeastern United States; Summer Flounder, Scup, and Black Sea Bass Fisheries; Interim 2012 Summer Flounder, Scup, and Black Sea Bass Specifications; 2012 Research Set-Aside Projects AGENCY: National...

  13. Electrofishing capture probability of smallmouth bass in streams

    Science.gov (United States)

    Dauwalter, D.C.; Fisher, W.L.

    2007-01-01

    Abundance estimation is an integral part of understanding the ecology and advancing the management of fish populations and communities. Mark-recapture and removal methods are commonly used to estimate the abundance of stream fishes. Alternatively, abundance can be estimated by dividing the number of individuals sampled by the probability of capture. We conducted a mark-recapture study and used multiple repeated-measures logistic regression to determine the influence of fish size, sampling procedures, and stream habitat variables on the cumulative capture probability for smallmouth bass Micropterus dolomieu in two eastern Oklahoma streams. The predicted capture probability was used to adjust the number of individuals sampled to obtain abundance estimates. The observed capture probabilities were higher for larger fish and decreased with successive electrofishing passes for larger fish only. Model selection suggested that the number of electrofishing passes, fish length, and mean thalweg depth affected capture probabilities the most; there was little evidence for any effect of electrofishing power density and woody debris density on capture probability. Leave-one-out cross validation showed that the cumulative capture probability model predicts smallmouth abundance accurately. ?? Copyright by the American Fisheries Society 2007.

  14. Historical development of entrainment models for Hudson River striped bass

    International Nuclear Information System (INIS)

    In the mid-1960s, concerns surfaced regarding entrainment and impingement of young-of-the-year (age-0) striped bass by electric power generating facilities on the Hudson River. These concerns stimulated the development of increasingly complex models to evaluate the impacts of these facilities. The earliest simplistic formulas, based on empirical data, proved inadequate because of conceptual shortcomings, incomplete development, and lack of data. By 1972, complex transport models based on biological and hydrodynamic principles had been developed and applied by scientists representing both the utilities and the government. Disagreements about the acceptability of these models spurred the development of even more complex models. The entrainment models stimulated the collection of substantial amounts of field data to define the spatial distributions and entrainment survival of early life stages. As the difficulties of accounting for the movement of early life stages from hydrodynamic principles became more evident and as more field data became available, simpler empirical modeling approaches became both practical and defensible. Both empirical and hydrodynamic modeling approaches were applied during the US Environmental Protection Agency's hearings on the Hudson River power case (1977-1980). The main lessons learned from the experience with entrainment-impingement modeling are that complex mechanistic models are not necessarily better than simpler empirical models for young fish, and that care must be taken to construct even the simple models correctly. 29 refs., 4 figs., 1 tab

  15. Is high pressure treatment able to modify the allergenicity of the largemouth bass allergens?

    Science.gov (United States)

    Liu, Chu-Yi; Tao, Sha; Liu, Rong; Chen, Fu-Sheng; Xue, Wen-Tong

    2012-12-01

    The aim of this paper is to study the influence of high pressure treatment on the structural changes and allergenicity of largemouth bass. We treated the allergens at 100, 200, 300 and 400 MPa for 15 min and at 300 MPa for 5, 10, 15, 20 and 30 min at 20 °C. The treated samples from largemouth bass were tested for their IgE-binding properties by combining Sodium dodecyl sulfate-Polyacrylamide gel electrophoresis (SDS-PAGE) with western blotting (WB) and enzyme-linked immunosorbent assay (ELISA). Circular dichroism analysis was performed to characterize the structural change. In summary, we can determine that the greatest structure changes were found for samples treated by 400 MPa for 15 min. High pressure treatment did change the structure, subunit composition and molecular weight of largemouth bass allergens, but it did not change the allergenicity of the allergens.

  16. Slow growth did not decouple the otolith size-fish size relationship in striped bass

    Science.gov (United States)

    Dickey, C.L.; Isely, J.J.; Tomasso, J.R.

    1997-01-01

    Eight-day-old striped bass Morone saxatilis (6.17-6.22 mm, total length) were fed twice daily at three feeding rates to produce three growth rates. Fish were sampled once per week for 4 weeks to determine total length and otolith radius. Feed ration treatments resulted in discrete size-classes of striped bass after 4 weeks with a 27% difference in mean length between the low and high feed ration treatments. No significant differences in slope or intercept for the regression of fish length on otolith radius were observed among treatments, suggesting that slow growth alone may not be sufficient to result in decoupling of the otolith size-fish size relationship in striped bass.

  17. Combining telephone surveys and fishing catches self-report: the French sea bass recreational fishery assessment.

    Directory of Open Access Journals (Sweden)

    Delphine Rocklin

    Full Text Available Fisheries statistics are known to be underestimated, since they are mainly based on information about commercial fisheries. However, various types of fishing activities exist and evaluating them is necessary for implementing effective management plans. This paper assesses the characteristics and catches of the French European sea bass recreational fishery along the Atlantic coasts, through the combination of large-scale telephone surveys and fishing diaries study. Our results demonstrated that half of the total catches (mainly small fish were released at sea and that the mean length of a kept sea bass was 46.6 cm. We highlighted different patterns of fishing methods and type of gear used. Catches from boats were greater than from the shore, both in abundance and biomass, considering mean values per fishing trip as well as CPUE. Spearfishers caught the highest biomass of sea bass per fishing trip, but the fishing rod with lure was the most effective type of gear in terms of CPUE. Longlines had the highest CPUE value in abundance but not in biomass: they caught numerous but small sea bass. Handlines were less effective, catching few sea bass in both abundance and biomass. We estimated that the annual total recreational sea bass catches was 3,173 tonnes of which 2,345 tonnes were kept. Since the annual commercial catches landings were evaluated at 5,160 tonnes, recreational landings represent 30% of the total fishing catches on the Atlantic coasts of France. Using fishers' self-reports was a valuable way to obtain new information on data-poor fisheries. Our results underline the importance of evaluating recreational fishing as a part of the total amount of fisheries catches. More studies are critically needed to assess overall fish resources caught in order to develop effective fishery management tools.

  18. The effects of bupropion on hybrid striped bass brain chemistry and predatory behavior.

    Science.gov (United States)

    Sweet, Lauren E; Bisesi, Joseph H; Lei, E N Y; Lam, Michael H W; Klaine, Stephen J

    2016-08-01

    Increased use of antidepressants has led to an increase in their detection in final treated wastewater effluents and receiving streams. Antidepressants are intended to modify human behavior by altering brain chemistry, and because of the high functional conservation of antidepressant target receptors in vertebrates, aquatic organisms may be at risk. The antidepressant bupropion is designed to alter brain norepinephrine and dopamine concentrations in humans. The objective of the present study was to understand if alteration of dopaminergic neurotransmitter concentrations in the hybrid striped bass (Morone saxatilis × Morone chrysops) brain by bupropion would alter this predator's ability to capture prey. The authors exposed hybrid striped bass to bupropion in a static system for 6 d, followed by a 6-d recovery period. During the present study's 12-d experiment, each hybrid striped bass was fed 4 unexposed fathead minnows every 3 d, and the time it took the hybrid striped bass to consume each of those 4 fathead minnows was quantified. After each feeding event, hybrid striped bass brains were harvested and analyzed for changes in several brain neurotransmitter concentrations, including serotonin, norepinephrine, dopamine, and many of their metabolites. Although bupropion altered the concentration of dopamine and many of the dopaminergic neurotransmitter metabolite concentrations in the brains on day 3 of the exposure, it did not alter the time to capture prey. This suggests that alteration of dopaminergic neurotransmitter concentrations in the hybrid striped bass brain does not alter a predator's ability to capture prey. Environ Toxicol Chem 2016;35:2058-2065. © 2016 SETAC. PMID:26748934

  19. Indigenous development and performance evaluation of BARC aerodynamic size separator (BASS)

    International Nuclear Information System (INIS)

    Commercially available cascade impactors, commonly used for aerodynamic size separation of aerosol particles, are based on the principle of inertial impaction. As of now, these instruments are imported at a cost of several lakhs of rupees; hence an effort has been made to develop an aerodynamic particle sizer indigenously in BARC. This unit, referred to as BARC Aerodynamic Size Separator (BASS), separates aerosols into seven size classes ranging from 0.53 μm to 10 μm and operates at a flow rate of 45 Ipm. Intercomparison studies between the standard Andersen Mark-II (Grasbey Andersen Inc.) impactor and BASS using nebulizer generated aerosols have consistently shown excellent performance by BASS in all respects. In particular, BASS yielded the parameters of polydisperse aerosols quite accurately. Experiments to evaluate the individual stage cut-off diameters show that these are within 8% of their designed value for all stages except the higher two stages which indicate about 30% lower values than the designed ones. The replotting of all the mass distribution data using the experimental cut-off diameters showed perfect lognormal fits, thereby indicating that these diameters are closer to the true stage cut-off diameters for BASS. The studies show that BASS will be suitable for determining the particle size distributions in the context of the radiological safety programmes of DAE. Being indigenous in design, it may be fabricated on a commercial scale at a cost far less than that of the imported units. Such a venture will greatly help several national programmes on atmospheric pollution being carried out by many laboratories and institutions across the country. (author)

  20. DIETS IN SEA BASS AND SEA BREAM REARING — DEVELOPMENT AND PROSPECTIVE

    Directory of Open Access Journals (Sweden)

    Lav Bavčević

    2006-07-01

    Full Text Available Sea bass feeding, in the early beginning of cage culture activity, was based on modified trout pellets and minced sardines. Soon–after knowledge on sea bass and sea bream nutritional needs has been rapidly improved. Recommended balance between digestible proteins and digestible energy (DP/DE in sea bass and sea bream diets according to different authors is between 19— 21 MJ/kg and 21–24 MJ/kg. Variations of optimal DP/DE in scientific publications in the past, seems to be reflected on significant variation of DP/DE in commercial diets of different producers. Simple method to estimate optimal amino acid balance in the diet is amino acid analysis of whole body protein. Fat content in feed for sea bass and sea bream is around 20 %. Required enrichment with essential fatty acids (especially HUFA n 3 is usually resolved when at least 7% of fish oil is added in the diet. Essential fatty acid (EFA relative content in farmed sea bass and sea bream muscle fat was found to be similar to the relative content of EFA in fish oil. Vitamins–mineral premixes are standardized and given in higher concentration only in special feeds which can be enriched with immunostimulatns, and used in specific circumstances to improve immunobalance. To establish new feed production for sea bass and sea bream rearing, beside needed knowledge of nutritive value of raw materials and seasonal variations in fish demand, is important to know growth dynamics of cultured organisms and, is the most important — market situation. Competition and increased problems with quality raw material supply are constantly increasing production cost effective value. Today the margin for income–outcome equilibrium are 20 000 t, with the accent that it will be higher in coming years.

  1. First haematic results for the sea bass (Dicentrarchus labrax metabolic profile assessment

    Directory of Open Access Journals (Sweden)

    Riccardo Bozzi

    2010-01-01

    Full Text Available The assessment of blood reference ranges of farmed fish can be extremely useful in improving production and productquality. A first attempt to establish the normality ranges for the most important haematochemical parameters of farmedsea bass was carried out by analysing the trend of Haematocrit, Glucose, Total Protein, Albumin, Globulin, TotalCholesterol and some electrolytes in 353 sea bass farmed with two different farming systems within 1 year. A strong seasonaleffect was found with regard to each parameter; the role of some environmental conditions was evaluated; andsome reference ranges were proposed for the culturing methods considered.

  2. Bass-Ihara Zeta functions for non-uniform tree lattices and class number asymptotics

    OpenAIRE

    Deitmar, Antonius; Kang, Ming-Hsuan

    2014-01-01

    It is shown that the Euler product giving the Bass-Ihara zeta function of a finite graph also converges in the case of a non-compact arith- metic quotient graph. Despite the infinite-dimensional setting, it turns out to be a rational function, generally with zeros and poles, in contrast to the compact case. The determinant formulas of Bass and Ihara hold true if one defines the determinant as limit of all finite principal minors. From this analysis, a prime geodesic theorem is derived, which,...

  3. Swimming muscles power suction feeding in largemouth bass.

    Science.gov (United States)

    Camp, Ariel L; Roberts, Thomas J; Brainerd, Elizabeth L

    2015-07-14

    Most aquatic vertebrates use suction to capture food, relying on rapid expansion of the mouth cavity to accelerate water and food into the mouth. In ray-finned fishes, mouth expansion is both fast and forceful, and therefore requires considerable power. However, the cranial muscles of these fishes are relatively small and may not be able to produce enough power for suction expansion. The axial swimming muscles of these fishes also attach to the feeding apparatus and have the potential to generate mouth expansion. Because of their large size, these axial muscles could contribute substantial power to suction feeding. To determine whether suction feeding is powered primarily by axial muscles, we measured the power required for suction expansion in largemouth bass and compared it to the power capacities of the axial and cranial muscles. Using X-ray reconstruction of moving morphology (XROMM), we generated 3D animations of the mouth skeleton and created a dynamic digital endocast to measure the rate of mouth volume expansion. This time-resolved expansion rate was combined with intraoral pressure recordings to calculate the instantaneous power required for suction feeding. Peak expansion powers for all but the weakest strikes far exceeded the maximum power capacity of the cranial muscles. The axial muscles did not merely contribute but were the primary source of suction expansion power and generated up to 95% of peak expansion power. The recruitment of axial muscle power may have been crucial for the evolution of high-power suction feeding in ray-finned fishes. PMID:26100863

  4. European sea bass, Dicentrarchus labrax, in a changing ocean

    Science.gov (United States)

    Pope, E. C.; Ellis, R. P.; Scolamacchia, M.; Scolding, J. W. S.; Keay, A.; Chingombe, P.; Shields, R. J.; Wilcox, R.; Speirs, D. C.; Wilson, R. W.; Lewis, C.; Flynn, K. J.

    2014-05-01

    Ocean acidification, caused by rising concentrations of carbon dioxide (CO2), is widely considered to be a major global threat to marine ecosystems. To investigate the potential effects of ocean acidification on the early life stages of a commercially important fish species, European sea bass (Dicentrarchus labrax), 12 000 larvae were incubated from hatch through metamorphosis under a matrix of two temperatures (17 and 19 °C) and two seawater pCO2 levels (ambient and 1,000 μatm) and sampled regularly for 42 days. Calculated daily mortality was significantly affected by both temperature and pCO2, with both increased temperature and elevated pCO2 associated with lower daily mortality and a significant interaction between these two factors. There was no significant pCO2 effect noted on larval morphology during this period but larvae raised at 19 °C possessed significantly larger eyes and lower carbon:nitrogen ratios at the end of the study compared to those raised under 17 °C. Similarly, when the incubation was continued to post-metamorphic (juvenile) animals (day 67-69), fish raised under a combination of 19 °C and 1000 μatm pCO2 were significantly heavier. However, juvenile D. labrax raised under this combination of 19 °C and 1000 μatm pCO2 also exhibited lower aerobic scopes than those incubated at 19 °C and ambient pCO2. Most studies investigating the effects of near-future oceanic conditions on the early life stages of marine fish have used incubations of relatively short durations and suggested that these animals are resilient to ocean acidification. Whilst the increased survival and growth observed in this study supports this view, we conclude that more work is required to investigate whether the differences in juvenile physiology observed in this study manifest as negative impacts in adult fish.

  5. Maternally transferred mercury in wild largemouth bass, Micropterus salmoides

    International Nuclear Information System (INIS)

    Maternal transfer of mercury in fish represents a potential route of elimination for adult females and a risk to developing embryos. To better quantify maternal transfer, we measured Hg in female largemouth bass (Micropterus salmoides) muscle and eggs from six waterbodies. Mercury in eggs from two waterbodies exceeded a US federal screening level (0.3 μg g−1) and was likely high enough to cause adverse reproductive effects. We found a curvilinear relationship between female and egg Hg. Fish with −1 Hg had low levels of Hg in eggs; those with Hg >0.37 μg g−1 showed a direct relationship between egg and muscle Hg (Log10 egg Hg = −1.03 + 1.18 * log10 muscle tissue Hg + 2.15 * (log10 muscle tissue Hg + 0.35)2). We also report higher maternal transfer (0.2–13.2%) and higher ratios of egg to muscle tissue Hg (4–52%) and egg to whole body Hg concentrations (7–116%) than previously observed for teleost fish. Highlights: •Previous work suggests maternal Hg transfer in teleosts is consistently low. •We provide evidence that teleosts can have high maternal Hg transfer. •Females with low Hg had similar and low concentrations of Hg in their eggs. •Females with high Hg had Hg in eggs that increased with somatic tissue Hg. •Egg Hg from high Hg females exceeded adverse effect levels. -- Capsule: Here we report higher maternal transfer and higher ratios of egg to muscle tissue Hg than previously observed for teleost fish

  6. Isolation of TDA-producing Phaeobacter strains from sea bass larval rearing units and their probiotic effect against pathogenic Vibrio spp. in Artemia cultures.

    Science.gov (United States)

    Grotkjær, Torben; Bentzon-Tilia, Mikkel; D'Alvise, Paul; Dourala, Nancy; Nielsen, Kristian Fog; Gram, Lone

    2016-05-01

    Fish-pathogenic Vibrio can cause large-scale crashes in marine larval rearing units and, since the use of antibiotics can result in bacterial antibiotic resistance, new strategies for disease prevention are needed. Roseobacter-clade bacteria from turbot larval rearing facilities can antagonize Vibrio anguillarum and reduce mortality in V. anguillarum-infected cod and turbot larvae. In this study, it was demonstrated that antagonistic Roseobacter-clade bacteria could be isolated from sea bass larval rearing units. In addition, it was shown that they not only antagonized V. anguillarum but also V. harveyi, which is the major bacterial pathogen in crustaceans and Mediterranean sea bass larvae cultures. Concomitantly, they significantly improved survival of V. harveyi-infected brine shrimp. 16S rRNA gene sequence homology identified the antagonists as Phaeobacter sp., and in silico DNA-DNA hybridization indicated that they could belong to a new species. The genomes contained genes involved in synthesis of the antibacterial compound tropodithietic acid (TDA), and its production was confirmed by UHPLC-TOFMS. The new Phaeobacter colonized live feed (Artemia) cultures and reduced Vibrio counts significantly, since they reached only 10(4)CFUmL(-1), as opposed to 10(8)CFUmL(-1) in non-Phaeobacter treated controls. Survival of V. anguillarum-challenged Artemia nauplii was enhanced by the presence of wild type Phaeobacter compared to challenged control cultures (89±1.0% vs 8±3.2%). In conclusion, TDA-producing Phaeobacter isolated from Mediterranean marine larviculture are promising probiotic bacteria against pathogenic Vibrio in crustacean live-feed cultures for marine fish larvae. PMID:26922490

  7. Indigenous development and performance evaluation of BARC aerodynamic size separator (BASS)

    CERN Document Server

    Singh, S; Khan, A; Mayya, Y S; Narayanan, K P; Purwar, R C; Sapra, B K; Sunny, F

    2002-01-01

    Commercially available cascade impactors, commonly used for aerodynamic size separation of aerosol particles, are based on the principle of inertial impaction. As of now, these instruments are imported at a cost of several lakhs of rupees; hence an effort has been made to develop an aerodynamic particle sizer indigenously in BARC. This unit, referred to as BARC Aerodynamic Size Separator (BASS), separates aerosols into seven size classes ranging from 0.53 mu m to 10 mu m and operates at a flow rate of 45 Ipm. Intercomparison studies between the standard Andersen Mark-II (Grasbey Andersen Inc.) impactor and BASS using nebulizer generated aerosols have consistently shown excellent performance by BASS in all respects. In particular, BASS yielded the parameters of polydisperse aerosols quite accurately. Experiments to evaluate the individual stage cut-off diameters show that these are within 8% of their designed value for all stages except the higher two stages which indicate about 30% lower values than the desig...

  8. Cellular and molecular immune responses of the sea bass (Dicentrarchus labrax) experimentally infected with betanodavirus

    DEFF Research Database (Denmark)

    Scapigliati, G.; Buonocore, F.; Randelli, E.;

    2010-01-01

    Naïve sea bass juveniles (38.4 ± 4.5 g) were intramuscularly infected with a sublethal dose of betanodavirus isolate 378/I03, followed after 43 days by a similar boosting. This infection resulted in an overall mortality of 7.6%. At various intervals, sampling of fish tissues was performed to inve...

  9. Laboratory experiment on bioaccumulation of 109Cd and 134Cs in white sea bass

    International Nuclear Information System (INIS)

    Laboratory radiotracer experiment was performed to study the bioaccumulation of 109Cd and 134Cs in the Malaysian common fish White sea bass (Lates calcarifer). The aim of this study was to compare the biokinetics of uptake these two contrasting radionuclides by White sea bass in laboratory condition scale. Experiments were designed to determine the processes controlling uptake of both radionuclides following exposure via seawater. In this study, the curve shapes of the uptake kinetic of 109Cd and 134Cs in White sea bass were slightly linear and gradually increased with increasing of exposure time but were not reach equilibrium in the period of the study of 21 days. This phenomenon can be concluded that radioelement concentrations of 109Cd and 134Cs, and exposure duration of this experiment may not adequately to reach steady-state condition for uptake kinetic of those radioelements in White sea bass. Furthermore, this was indicated that the uptake rate of 109Cd was 1.79 times faster than 134Cs due to some factors may probably influenced the output of this experiment such as different element accumulation strategies, physiological, behavior of radioelements, for example. (author)

  10. 78 FR 18931 - Proposed Establishment of Class E Airspace; Bass Harbor, ME

    Science.gov (United States)

    2013-03-28

    ...'' under DOT Regulatory Policies and Procedures (44 FR 11034; February 26, 1979); and (3) does not warrant... continues to read as follows: Authority: 49 U.S.C. 106(g); 40103, 40113, 40120; E.O. 10854, 24 FR 9565, 3... Federal Aviation Administration 14 CFR Part 71 Proposed Establishment of Class E Airspace; Bass Harbor,...

  11. Laboratory approaches to understanding gonadal development and abnormalities in wild-caught smallmouth bass (Micropterus dolomieu)

    Science.gov (United States)

    Our previous work reported smallmouth bass in Northeastern Minnesota rivers and lakes with a prevalence of testicular oocytes (TOs) ranging from 7 to 57%, which is consistent with findings reported in other U.S. river systems. While it is often presumed that TOs are caused by ex...

  12. BIOACCUMULATION AND AQUATIC SYSTEM SIMULATOR (BASS) USER'S MANUAL BETA TEST VERSION 2.1

    Science.gov (United States)

    BASS (Bioaccumulation and Aquatic System Simulator) is a Fortran 95 simulation program that predicts the population and bioaccumulation dynamics of age-structured fish assemblages that are exposed to hydrophobic organic pollutants and class B and borderline metals that complex wi...

  13. 77 FR 76942 - 2013-2014 Summer Flounder and Scup Specifications; 2013 Black Sea Bass Specifications...

    Science.gov (United States)

    2012-12-31

    ... specifications for these fisheries was provided in the proposed specifications (77 FR 68723; November 16, 2012... rule (77 FR 68723). Comment 1: One commenter suggested that the quotas should be reduced by 50 percent... and Scup Specifications; 2013 Black Sea Bass Specifications; Preliminary 2013 Quota Adjustments;...

  14. 78 FR 32355 - Establishment of Class E Airspace; Bass Harbor, ME

    Science.gov (United States)

    2013-05-30

    ... Bass Harbor, ME (78 FR 18931). Interested parties were invited to participate in this rulemaking effort... Procedures (44 FR 11034; February 26, 1979); and (3) does not warrant preparation of a Regulatory Evaluation...: Authority: 49 U.S.C. 106(g); 40103, 40113, 40120; E.O. 10854, 24 FR 9565, 3 CFR, 1959-1963 Comp., p....

  15. 75 FR 17618 - Fisheries of the Northeastern United States; Black Sea Bass Recreational Fishery; Emergency Rule...

    Science.gov (United States)

    2010-04-07

    ... effective October 5, 2009 (74 FR 51092), for a period of 180 days. This closure was necessary as the... implement the closure, and requires correction. Thus, this action is correcting the October 5, 2009 (74 FR... United States; Black Sea Bass Recreational Fishery; Emergency Rule Correction and Extension...

  16. Low frequency sound reproduction in irregular rooms using CABS (Control Acoustic Bass System)

    DEFF Research Database (Denmark)

    Celestinos, Adrian; Nielsen, Sofus Birkedal

    2011-01-01

    Early investigations on low frequency sound reproduction in rectangular rooms using CABS (Controlled Acoustic Bass System) have shown good results on simulations and measurements in real rooms. CABS takes the advantage of having a rectangular room with parallel walls. By using two low frequency l...

  17. Comparison of tank treatments with copper sulfate and potassium permanganate for sunshine bass with ichthyobodosis

    Science.gov (United States)

    The biflagellated single-cell parasite Ichthyobodo nectator can cause significant losses among fish populations, particularly those cultured in tanks. Potassium permanganate and CuSO4 treatments were evaluated against a naturally-occurring I. nectator infestation on sunshine bass raised in tanks. F...

  18. Laboratory efficacy of florfenicol against Streptococcus iniae infection in sunshine bass

    Science.gov (United States)

    An experimental feeding trial was performed to evaluate the efficacy of florfenicol (FF) in controlling Streptococcus iniae infection in sunshine bass (SB). Doses of FF tested were 0, 5, 10, 15 and 30 mg active ingredient per kilogram of fish body weight (BW) per day. Administration of medicated f...

  19. Phytoplankton succession in sunshine bass fry ponds and the effect of Aquashade®

    Science.gov (United States)

    Phytoplankton management in aquaculture ponds is very critical in maintaining good quality water for culturing fish especially during the fry and fingerling stages. Though much is known about succession in catfish ponds, that is not the case for sunshine bass ponds. This study was designed to look a...

  20. Amen-break : Från ett samplat trumbreak till nya trumkomp i drum'n'bass-musik.

    OpenAIRE

    Björni, Patrik

    2012-01-01

    Drum’n’bass är en genre inom elektronisk dansmusik som utvecklades under 1990-talet. Genren kännetecknas av rytmiskt komplexa trumkomp. Syftet med detta arbete är att ge en uppfattning av trumkomp, vilka kallas för breakbeats i drum’n’bass-musik. Med hjälp av litteratur har jag forskat i historien om drum’n’bass för att ge en uppfattning om hur genren utvecklades och dess bakgrund. Breakbeats är skapade genom att sampla och bearbeta trumkomp från äldre funk-och soullåtar med elektronisk...

  1. Diel behavior of adult striped bass using tailwater habitat as summer refuge

    Science.gov (United States)

    Young, S.P.; Isely, J.J.

    2007-01-01

    General patterns of summer diel distribution and movement were identified for adult striped bass Morone saxatilis using tailwater habitat influenced by the diel operation cycle of a hydroelectric dam during the summers of 2003 and 2004. Striped bass distribution within the tailwater was similar during each diel-tracking event and across both summers. The majority of fish remained within the tailwater the entire summer; however, some made periodic excursions to and from the tailwater throughout the summer. Further, most striped bass were located within 0.5 km of Richard B. Russell Dam during all stages of operation on all occasions - probably because of the constant availability of optimal habitat during all three stages of operation on all diel-tracking events. The diel cycle of dam operation, which included pumped storage during each summer, did not degrade tailwater habitat below optimal conditions, according to summer habitat suitability index values for inland adult striped bass. Movement was significantly higher during hydroelectric generation operations than during no-generation and pumped storage periods in summer 2003; this difference was not apparent during summer 2004. Mean absolute movement peaked during hydroelectric generation on six of eight diel-tracking events. During both summers, movement was directed up-reservoir during no-generation and generation periods and down-reservoir during pumped storage. Mean total daily movement rates ranged from 0.59 to 4.04 km/d and were greater than those previously estimated from bimonthly sampling for this population. Total daily movement rate peaked during the first tracking event each summer and then declined as summer progressed. These findings suggest that hydroelectric discharges affect adult striped bass behavior, but the effects are not adverse as long as habitat is not degraded by hydroelectric facility operations. ?? Copyright by the American Fisheries Society 2007.

  2. Mercury and selenium concentrations in largemouth bass and other fishes of the Lower Suwannee National Wildlife Refuge

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — From March 22 to April 7, 1990, 71 largemouth bass (Micropterus salmoides) and four other fish species (total of seven fish) were collected from ten locations on or...

  3. Metal contaminants in Largemouth bass (Micropterus salmoides) collected in large dams from Tejo River basin and small irrigation dams

    OpenAIRE

    L.P. Andrade; Antunes, P.; Paulo, L; Pereira, M. E.; A.M. Rodrigues

    2012-01-01

    Largemouth bass (Micropterus salmoides) is a very important fresh water fish in the Portuguese regional cuisine mainly in the countryside (Central region and north Alentejo). Because there’s no aquaculture industry, all eaten largemouth bass in Portugal are collected in large dams (Basins of Tejo and Guadiana rivers) and small irrigation dams. For decades, the Tejo River received environmental pollutants from non-point and point sources that included intensive agriculture, industrial entities...

  4. Dental and orthopedic effects of Bass and Herbst therapy for correction of severe class II division 1 malocclusions

    OpenAIRE

    Ömblus, Jane

    1996-01-01

    Ömblus J (1996). Dental and orthopedic effects of Bass and Herbst therapy for correction of severe Class 1I division l malocclusions. Thesis, Karolinska Institutet. Functional appliance therapy to correct Class II division I malocclusions has been a subject of debate throughout this century. The aims of this thesis, based on six publications, were to evaluate the dental and orthopedic effects of Bass appliance therapy in relation to treatment intensity, age, an...

  5. Dietary probiotic live yeast modulates antioxidant enzyme activities and gene expression of sea bass (Dicentrarchus labrax) larvae

    OpenAIRE

    Tovar-ramirez, D.; Mazurais, David; Gatesoupe, J. F.; Quazuguel, Patrick; Cahu, Chantal; Zambonino-infante, J. L.

    2010-01-01

    The main goal of this work was to determine the effect of dietary live yeast Debaryomyces hansenii on the enzymatic antioxidative status of sea bass Dicentrarchus labrax larvae. Growth, activity and expression of the main antioxidative enzymes: catalase (CAT), glutathione peroxidase (GPX) and superoxide dismutase (SOD), and heat shock protein (HSP70) were measured in sea bass larvae at 23 and 48 days after hatching. Larvae were fed on two microdiets: group one, fed microdiet containing live y...

  6. Effect of stocking density on growth and survival of spotted sand bass Paralabrax maculatofasciatus larvae in a closed recirculating system

    OpenAIRE

    Álvarez González, Carlos; Ortiz Galindo, José Luis; Dumas, Silvie; Martínez Díaz, Sergio Francisco; Hernández Ceballos, Dora Esther; Grayeb del Alamo, Tanos; Moreno Lagorreta, Manuel; Peña Martínez, Renato; Civera Cerecedo, Roberto

    2001-01-01

    The effect on growth and survival of the initial stocking density (50, 100, 150, and 200 larvae/ L) in larval rearing of spotted sand bass was evaluated over 30 d in a closed recirculating system. Larvae were fed with rotifers, copepods, nauplii and adult Artemia, and spotted sand bass yolk-sac larvae. Water quality was monitored daily. The notochordal or standard length of sampled larvae was measured by image analysis. Specific growth rates at each density were compared by covariance analysi...

  7. Effects of incremental increases in silt load on the cardiovascular performance of riverine and lacustrine rock bass, Ambloplites rupestris

    International Nuclear Information System (INIS)

    Rock bass (Ambloplites rupestris) are a widespread centrarchid species with both riverine and lacustrine populations. After precipitation events, rivers often carry elevated silt loads, where as lakes generally remain free from suspended silt and sediment. To examine the physiological effects of silt on rock bass, we conducted a series of experiments using fish from Lake Opinicon and the Grand River in Ontario. Ultrasonic Doppler flow probes were surgically affixed around the ventral aorta to monitor cardiovascular performance. After recovery from surgery replicated treatment groups were exposed to incremental increases in silt load (made from bentonite slurry), while cardiac output and its two components, heart rate and stroke volume, were measured simultaneously. Although both groups of rock bass responded significantly to low concentrations of silt (10 NTU), the response by riverine rock bass was rapidly extinguished by acclimation or physiological adjustment. Compensatory mechanisms to minimize cardiac (and respiratory) disruption attributable to increases in suspended silt appear to be inherent in rock bass of riverine origin. These fish appear to fully compensate for interference in gas exchange at the gill surfaces 60 min after initial exposure. In contrast, individual lacustrine rock bass were highly variable in their response to elevated silt concentrations. Changes in stroke volume and cardiac output suggested no clear compensatory mechanism or strategy to cope with increased silt levels. - Cardiovascular compensation in fish exposed to elevated levels of silt

  8. MODELISATION, CONCEPTION ET COMMANDE DE GENERATRICES A RELUCTANCE VARIABLE BASSE VITESSE

    OpenAIRE

    Moreau, Luc

    2005-01-01

    Les travaux présentés portent sur la conception et la commande d'un ensemble convertisseur-génératrice basse vitesse pour les applications éoliennes. Une génératrice 10kW, 50 tr/min (contrainte typique d'une éolienne autonome) est étudiée. La machine à réluctance variable (MRV) à plots dentés et à grand nombre de dents est retenue car elle est bien adaptée à un fonctionnement à basse vitesse de rotation permettant la simplification ou la suppression du multiplicateur de vitesse utilisé avec l...

  9. Interplay Between Amphioxus Complement with Sea Bass Macrophages: Opsonic Activity of Amphioxus Humoral Fluids

    Institute of Scientific and Technical Information of China (English)

    PAN Junli; LIU Min; ZHANG Shicui

    2011-01-01

    Previous studies have shown the existence of a complement system in the amphioxus Branchiostoma japonicum.However,whether it has an opsonic activity similar to that of vertebrates remains unknown.We demonstrated that the humoral fluid (HF)of amphioxus promoted the phagocytosis of yeast cells with sea bass (Lateolabraxjaponicus) macrophages,whereas the C3-depleted and heated HF significantly lost the phagocytosis-promoting capacity.In addition,the precipitation of factor B (Bf) led to a marked loss of opsonic activity.Moreover,C3 fragments in the HF were found to bind to yeast cell surfaces.The results indicate that the amphioxus complement system is an important element involved in the opsonic activity,which promotes the sea bass macrophage phagocytosis by tagging yeast cells with C3 fragments via the activation of alternative complement pathway.

  10. Plasma osmotic and electrolyte concentrations of largemouth bass from some acidic Florida lakes

    Energy Technology Data Exchange (ETDEWEB)

    Canfield, D.E. Jr.; Maceina, M.J.; Nordlie, F.G.; Shireman, J.V.

    1985-05-01

    Five acidic clear (pH 3.7-4.9), three acidic colored (pH 4.1-4.6), and three neutral (pH 6.9-7.3) north-central Florida lakes were surveyed in 1983 to determine plasma osmotic and electrolyte concentrations, growth, and coefficients of condition for largemouth bass Micropterus salmoides floridanus. Plasma osmotic concentrations averaged greater than 273 milliosmoles/kg in fish from acidic colored and circumneutral lakes, but averaged less than 269 milliosmoles/kg in four of the acidic clear lakes. Growth and coefficients of condition of largemouth bass > 305 mm total length in the acidic lakes were significantly lower than in the neutral lakes. Reductions in fish growth and condition, however, could be related to either acidic conditions or lake trophic status. 29 references, 3 tables.

  11. BASS 4: a software system for ergonomic design and evaluation of working hours

    Directory of Open Access Journals (Sweden)

    Carsten Schomann

    2004-12-01

    Full Text Available OBJECTIVE: To extend an existing computer programme for the evaluation and design of shift schedules (BASS 3 by integrating workload as well as economic aspects. METHODS: The redesigned prototype BASS 4 includes a new module with a suitable and easily applicable screening method (EBA for the assessment of the intensity of physical, emotional and cognitive workload components and their temporal patterns. Specified criterion functions based on these ratings allow for an adjustment of shift and rest duration according to the intensity of physical and mental workload. Furthermore, with regard to interactive effects both workload and temporal conditions, e.g. time of day, are taken into account. In a second new module, important economic aspects and criteria have been implemented. Different ergonomic solutions for scheduling problems can now also be evaluated with regard to their economic costs. RESULTS: The new version of the computer programme (BASS 4 can now simultaneously take into account numerous ergonomic, legal, agreed and economic criteria for the design and evaluation of working hours. CONCLUSIONS: BASS 4 can now be used as an instrument for the design and the evaluation of working hours with regard to legal, ergonomic and economic aspects at the shop floor as well as in administrative (e.g. health and safety inspection and research problems.OBJETIVOS: Expandir um programa computacional existente para planejamento e avaliação dos horários de turnos (BASS 3 por meio da incorporação da carga de trabalho e características econômicas. MÉTODOS: O protótipo BASS 4 contém um novo módulo com um método de triagem (EBA conveniente e de fácil aplicação para a avaliação da intensidade dos componentes físico, emocional e cognitivo da carga de trabalho e seus padrões temporais. O uso de critérios específicos com base nestas avaliações possibilita ajustar a duração do turno e do descanso de acordo com a intensidade da carga de

  12. Vaccination of European sea bass fry through bioencapsulation of Artemia nauplii

    OpenAIRE

    Chair, M.; Gapasin, R.S.J.; Dehasque, M.; Sorgeloos, P.

    1994-01-01

    European sea bass (Dicentrarchus labrax) fry vaccinated orally by bioencapsulation in Artemia nauplii or by bath method exhibited better performance than control fish in terms of growth, food conversion and resistance to stress. The comparable survival between vaccinated and non-vaccinated animals suggests that vaccination methods are stressful. The present study shows that oral vaccination can be used to enhance growth in fish fry.

  13. Seasonal variations of the humoral immune parameters of European sea bass (Dicentrarchus labrax L.)

    OpenAIRE

    Valero, Y. (Yulema)

    2014-01-01

    Seasonal cycles, mainly due to great variations in the light duration and temperature, are important and modulate several aspects of the animal behavior. In the case of poikilotherms animals such as fish this is very relevant. Thus, temperature changes fish immunity and affects disease resistance. We evaluate in this work the season variations of the European sea bass (Dicentrarchus labrax) humoral innate parameters focusing on winter months, at which the culture of this specie is more diffic...

  14. Wind energy in ''Basse Normandie'': the energies of the sustainable development

    International Nuclear Information System (INIS)

    This paper brings together the main topics discussed during the 4. colloquium on the wind energy: the french lateness concerning the wind energy development, the regulatory framework concerning the wind turbines implementation sites, the wind energy situation in ''Basse Normandie'', the offshore wind energy, the site of Sortosville-en-Beaumont, the public relations, the employment and an analysis of some rumors and prejudices. (A.L.B.)

  15. Application of a bioenergetics model for hatchery production: Largemouth bass fed commercial diets

    Science.gov (United States)

    Csargo, Isak J.; Michael L. Brown; Chipps, Steven R.

    2012-01-01

    Fish bioenergetics models based on natural prey items have been widely used to address research and management questions. However, few attempts have been made to evaluate and apply bioenergetics models to hatchery-reared fish receiving commercial feeds that contain substantially higher energy densities than natural prey. In this study, we evaluated a bioenergetics model for age-0 largemouth bass Micropterus salmoidesreared on four commercial feeds. Largemouth bass (n ≈ 3,504) were reared for 70 d at 25°C in sixteen 833-L circular tanks connected in parallel to a recirculation system. Model performance was evaluated using error components (mean, slope, and random) derived from decomposition of the mean square error obtained from regression of observed on predicted values. Mean predicted consumption was only 8.9% lower than mean observed consumption and was similar to error rates observed for largemouth bass consuming natural prey. Model evaluation showed that the 97.5% joint confidence region included the intercept of 0 (−0.43 ± 3.65) and slope of 1 (1.08 ± 0.20), which indicates the model accurately predicted consumption. Moreover model error was similar among feeds (P = 0.98), and most error was probably attributable to sampling error (unconsumed feed), underestimated predator energy densities, or consumption-dependent error, which is common in bioenergetics models. This bioenergetics model could provide a valuable tool in hatchery production of largemouth bass. Furthermore, we believe that bioenergetics modeling could be useful in aquaculture production, particularly for species lacking historical hatchery constants or conventional growth models.

  16. Water chemistry characterization and component performance of a recirculating aquaculture system producing hybrid striped bass

    OpenAIRE

    Easter, Christopher

    1992-01-01

    Eight identical and independent pilot scale recirculating aquaculture production systems were populated with fingerling hybrid striped bass (Morone chrysops female x Morone saxatilis male). Three population densities were established with two replicates at 132 fishlm3 and three replicates each at 66 and 33 fishlm3. Water chemistry and water quality characteristics were monitored throughout the 228 day growth trial for all eight systems. A system component performance analys...

  17. Summer habitat selection by striped bass, Morone Saxatilis, in Cherokee Reservoir, Tennessee, 1977

    Energy Technology Data Exchange (ETDEWEB)

    Waddle, H.R.; Coutant, C.C.; Wilson, J.L.

    1980-02-01

    Summer habitat selection patterns of 18 adult striped bass (Morone saxatilis) in Cherokee Reservoir were monitored with externally attached temperature-sensing acoustic or radio transmitters from June through September 1977. Mortalities of adult striped bass in this reservoir were hypothesized to be related to high summer temperatures and low dissolved oxygen (DO). The inhabited areas or refuges differed from noninhabited areas by maintaining temperatures less than or equal to 22 C and DO concentrations greater than 5 mg/liter. Total water hardness, pH, and water transparency were not significantly different among refuges and noninhabited areas. Movement of fish outside refuges occurred more frequently and for longer periods during June when the summer pattern of high temperatures and low DO was less severe. Fish experienced temperatures between 15 and 27 C with mean temperatures of individuals ranging from 18.5 to 22.0 C. Several tagged fish migrated outside the refuges and selected the lowest available temperature, generally near 21 C, even though DO concentrations at these temperatures were 3 mg/liter or less. Long-term survival of tagged and nontagged fish outside refuges was undetermined because no fish were tracked outside a refuge for more than 12 days without being lost. This study indicates that temperature strongly influences the behavior of striped bass and that adults of this species may have a thermal preferendum of approximately 21 C.

  18. Near infrared reflectance spectroscopy (NIRS characterization of European sea bass (Dicentrarchus labrax from different rearing systems

    Directory of Open Access Journals (Sweden)

    Andrea Santulli

    2010-01-01

    Full Text Available The present study aimed to predict by NIRS the proximate chemical composition and some carcass traits of sea bass coming from 11 farms with different rearing systems (extensive, intensive in land-based basins, sea cages and located in northern (Friuli, Veneto, central (Tuscany and southern (Puglia and Sicily Italy. NIRS analysis of freeze dried sea bass fillets gave fairly good predictions of slaughter weight and fillet yield (R2cv=0.48-0.55, while results for carcass yield were poor. NIRS analysis was highly predictive for the condition factor (R2cv=0.790, SECV=0.09 and for water, ether extract and gross energy showing high correlations (R2cv>0.90 with NIR spectral infor- mation and high accuracy (SECV=0.67%, 0.46% and 0.38 kJ/g for water, ether extract and energy, respectively. Crude protein prediction showed lower performance, even if still good, compared to pre- vious variables (R2cv=0.734, SECV=0.34. The score plot of principal component analysis showed in- tensively-reared sea bass separated from extensively reared fish.

  19. 137Cs elimination by chronically-contaminated largemouth bass (Micropterus salmoides)

    International Nuclear Information System (INIS)

    The temperature-dependent 137Cs biological half-times (Tb) of lifetime-exposed largemouth bass (Micropterus salmoides) from a nuclear cooling reservoir at the US Department of Energy's Savannah River Site were calculated from whole-body measurements of live fish and compared with literature records for acutely and chronically-contaminated fish. The Tb's of the bass averaged 322 d, 225 d, and 140 d at 15, 20, and 26 C, respectively. These mean Tb's were 1.7 to 2.5 times longer than would be expected for acutely contaminated fish, and 1.2 to 1.8 times longer than those predicted for fish at steady-state with their environment according to recent models. This slower elimination did not appear to result from slower elimination from skeletal muscle compared with other soft tissues, in that the muscle to whole-body 137Cs concentration ratios after the elimination period were similar to those of freshly-caught bass. Their results suggested that elimination rates estimated from the terminal elimination components of acutely-dosed fish may not reflect the elimination rates of fish exposed to contaminants throughout their lifetime, even when care is taken to allow sufficient time for absorption of the dose

  20. Histologic and molecular characterization of Edwardsiella piscicida infection in largemouth bass (Micropterus salmoides).

    Science.gov (United States)

    Fogelson, Susan B; Petty, Barbara D; Reichley, Stephen R; Ware, Cynthia; Bowser, Paul R; Crim, Marcus J; Getchell, Rodman G; Sams, Kelly L; Marquis, Hélène; Griffin, Matt J

    2016-05-01

    The genus Edwardsiella is composed of a diverse group of facultative anaerobic, gram-negative bacteria that can produce disease in a wide variety of hosts, including birds, reptiles, mammals, and fish. Our report describes the isolation and identification of Edwardsiella piscicida associated with chronic mortality events in 2 separate captive largemouth bass (Micropterus salmoides) populations in New York and Florida. Wet-mount biopsies of skin mucus, gill, kidney, and spleen from several affected largemouth bass contained significant numbers of motile bacteria. Histologic examination revealed multifocal areas of necrosis scattered throughout the heart, liver, anterior kidney, posterior kidney, and spleen. Many of the necrotic foci were encapsulated or replaced by discrete granulomas and associated with colonies of gram-negative bacteria. Initial phenotypic and matrix-assisted laser desorption ionization-time of flight mass spectrometric analysis against existing spectral databases of recovered isolates identified these bacteria as Edwardsiella tarda Subsequent molecular analysis using repetitive sequence mediated and species-specific PCR, as well as 16S rRNA, rpoB, and gyrB sequences, classified these isolates as E. piscicida As a newly designated taxon, E. piscicida should be considered as a differential for multiorgan necrosis and granulomas in largemouth bass. PMID:26951328

  1. Biomarker Benchmarks: Reproductive and Endocrine Biomarkers in Largemouth Bass and Common Carp from United States Waters

    Science.gov (United States)

    Goodbred, Steven L.; Smith, Stephen B.; Greene, Patricia S.; Rauschenberger, Richard H.; Bartish, Timothy M.

    2007-01-01

    The U.S. Geological Survey (USGS) has developed a national database and report on endocrine and reproductive condition in two species of fish collected in U.S. streams and rivers. This information provides scientists with a national basis for comparing results of endocrine measurements in fish from individual sites throughout the country, so that scientists can better ascertain normal levels of biomarkers. The database includes information on several measures of reproductive and endocrine condition for common carp and largemouth bass. Data summaries are provided by reproductive season and geographic region. A national-scale reconnaissance investigation was initiated in 1994 by the USGS that utilized a suite of biological assays (biomarkers) as indicators of reproductive health, and potentially, endocrine disruption in two widely distributed species of teleost (bony) fish, largemouth bass (Micropterus salmoides) and common carp (Cyrinus carpio). The suite of assays included plasma sex-steroid hormones, stage of gonadal development, and plasma vitellogenin, an egg protein that indicates exposure to estrogenic compounds when found in male fish. More than 2,200 common carp and 650 largemouth bass were collected at 119 rivers and streams (fig. 1).

  2. MODIFICATION OF PHOTOPERIOD REDUCES EARLY SEXUAL MATURATION IN JUVENILE SEA BASS (Dicentrarchus labrax (Hormonal aspects.

    Directory of Open Access Journals (Sweden)

    M. Carrillo

    2011-04-01

    Full Text Available The effect of short-term exposure to continuous light (LL to reduce early male sexual maturation was investigated in the sea bass. Four LL light regimes (1 or 2-month duration were established during the pregametogenesis, whereas a simulated natural photoperiod (SNP and a long-term exposure group (12-month duration to LL were kept as controls according to previous trial in this species. Exposure to different LL treatments induced a differential response on the sex-steroid levels. Particularly, significant differences (ANOVA, n=20, P<0.05 in the levels of 11-KT were observed during the peak of reproductive period (February in groups 4 and 5 (control in comparison with groups 1, 2, 3, 6. These results suggested the role of this androgen as a putative threshold determining early puberty in the sea bass as previously reported. Profiles of T were similar during whole experimental period. E2 levels at the beginning of pregametogenesis (September presented significant differences between SNP groups (5 and 2 and LL treatments groups (1, 3, 4, 6. Thus, the identification of a short-term exposure to LL approach can be of interest for sea bass aquaculture, since it could reduce the presence of early sexual maturing males under farming conditions.

  3. A simple model for predicting survival of angler-caught and released largemouth bass

    Science.gov (United States)

    Wilde, G.R.; Pope, K.L.

    2008-01-01

    We conducted a controlled experiment in the laboratory to assess the influence of anatomical hooking location and water temperature on survival of angler-caught and released largemouth bass Micropterus salmoides. Survival was 98% (58 of 59 fish) among fish that were hand-hooked within the oral cavity (including the gills), whereas survival was 66% (33 of 50 fish) among fish that were hand-hooked in the esophagus. Survival of hooked fish was not significantly influenced by water temperature (7-27??C) or the hooking location X water temperature interaction. We combined our results with prior research to develop a predictive model of largemouth bass survival, which was 98.3% (SD = 1.87%) for fish hooked in the oral cavity and 55.0% (SD = 9.70%) for fish hooked in the esophagus. The model is valid for water temperatures ranging from 7??C to 27??C and allows one to estimate, with known precision, the survival of angler-caught and released largemouth bass without the need for controlled studies or for holding fish in pens or cages to assess delayed mortality. ?? Copyright by the American Fisheries Society 2008.

  4. Element content in cultured and wild sea bass (Dicentrarchus labrax from the Adriatic Sea

    Directory of Open Access Journals (Sweden)

    Žvab Rožič P.

    2013-04-01

    Full Text Available The aim of this study was to determine the levels of potentially toxic elements in cultured and wild fish tissues and to assess their risk for human health. For this purpose, sea bass specimens (Dicentrarchus labrax were sampled in selected fish farm and three other locations along the eastern Adriatic coast. Ranges of element concentrations in sea bass muscles were 1.60-4.46 ppm for As, 0.001-0.079 ppm for Cd, 0.14-49.10 ppm for Cr, 1.38-4.85 ppm for Cu, 0.11-1.31 ppm for Hg, 0.01-0.65 ppm for Pb and 21.9-136.0 ppm for Zn. Mean Cd, Cr, Cu, Hg, Pb, and Zn concentrations in commercially interesting cultured fish samples were below the permissible levels, while mean As values slightly exceed those limits. In wild fishes mean Cd, Cu, Pb and Zn concentrations were below the recommended limits, for As, Cr and Hg the mean values were higher. The smallest cultured sea bass samples showed As, Cr, Pb, and Zn concentrations exceeding the recommended limits but values decreased with fish size. Therefore, the metal concentrations in commercial fishes showed no threat for human consumption.

  5. Abundance and Distribution of Walleye, Northern Squawfish, and Smallmouth Bass in John Day Reservoir, 1984-1985 Progress Report.

    Energy Technology Data Exchange (ETDEWEB)

    Beamesderfer, Raymond C.

    1985-12-01

    Sampling was conducted in John Day Reservoir to collect walleye, northern squawfish and smallmouth bass. Changes in distributions during sampling were characterized from changes in catch per unit effort (CPUE) in sampling areas. Observed movements of marked and radiotagged fish were examined and used to define discrete populations. Abundances were estimated using a modified Schnabel multiple mark and recapture estimator. Abundance estimates were corrected for angler harvest, size specific vulnerability to gear, recruitment due to growth and tag loss during sampling. Age composition of catch was determined to characterize relative contributions of various year classes to the populations. Ages at which fish were fully recruited to gear were defined by catch curves. Survival of fully recruited year classes was calculated from differences in CPUE's between 1984 and 1985. Mean length at age was estimated and used to determine age specific incremental growth. Eighty-eight percent of walleye were caught in McNary tailrace or Irrigon-Paterson, whereas 95% of smallmouth bass were caught from Irrigon-Paterson to the John Day forebay. Abundances of walleye and northern squawfish with fork lengths greater than 250 mm and smallmouth bass with fork lengths greater than 200 mm were estimated to be 16,219, 95,407, and 11,259. Anglers harvested an estimated 235 walleye, 2004 northern squawfish and 4383 smallmouth bass during the sampling season. Six-year-old walleye, 4-year-old northern squawfish and 3-year-old smallmouth bass were most abundant in catches. Walleye and smallmouth bass were fully recruited to sampling gear by age 3. Age at which northern squawfish were fully recruited was uncertain. Mean survival was 46.1% for walleye and 46.5% for northern squawfish. Mean smallmouth bass survival was 46.5% in the lower and 43.7% in the upper reservoir.

  6. Virulence and molecular typing of Vibrio harveyi strains isolated from cultured dentex, gilthead sea bream and European sea bass.

    Science.gov (United States)

    Pujalte, M J; Sitjà-Bobadilla, A; Macián, M C; Belloch, C; Alvarez-Pellitero, P; Pérez-Sánchez, J; Uruburu, F; Garay, E

    2003-06-01

    Vibrio harveyi was isolated from internal organs or ulcers of diseased and apparently healthy gilthead sea bream (Sparus aurata) and European sea bass (Dicentrarchus labrax) cultured in several fish farms located on the Spanish Mediterranean coast. The prevalence of the bacterium was significantly higher in European sea bass than in gilthead sea bream, and was closely related to the season in both fish species, occurring almost exclusively on warm months (June to November). After phenotypic characterization, a selection of forty five isolates from gilthead sea bream, sea bass, and several isolates previously obtained from common dentex (Dentex dentex) of the same area, were molecularly typed by automated ribotyping and random amplified polymorphic DNA (RAPD) analysis. Cluster analysis of data established 8 RAPD types and 13 ribotypes among wild isolates, and the combination of both techniques allowed to define fourteen different groups and a clear discrimination of all outbreaks and samplings. Several strains isolated from diseased gilthead sea bream and sea bass and also from asymptomatic sea bream, were tested for virulence in both fish species by intracoelomic injection. All the isolates (11) were pathogenic for sea bass, with nine out of the eleven LD50 values ranging from 1.5 x 10(5) to 1.6 x 10(6) cfu/fish. Gilthead sea bream was unaffected by the seven tested strains, even by those more virulent for sea bass, and only one strain caused a 10% mortality at 4.2 x 10(7) cfu/fish. This is the first report on virulence of V. harveyi for sea bass. PMID:12866856

  7. Die faktorstruktuur van Bass se veelfaktor- leierskapsvraelys in die Suid-Afrikaanse konteks

    Directory of Open Access Journals (Sweden)

    C .P. Ackermann

    2000-06-01

    Full Text Available The factor structure of Bass's Multifactor Leadership Questionnaire in the South African context. The aim of the study was to determine whether the factor structure of Bass's Multifactor Leadership Questionnaire (MLQ, as a measure of transformational leadership, could be replicated within the South African context. The MLQ was chosen not only because it promised to be a valid and reliable measuring instrument of the construct in question, but also due to the fact that there was an urgent need for such an instrument in the management of human resources within organisations undergoing transformation. The MLQ was administered to 406 subjects within the military context and was subjected to factor analysis and item analysis. The factor analysis yielded three factors, namely transformational leadership, transactional leadership and avoidance of leadership ("laissez faire" leadership. The reliabilities of the scales were determined by means of Cronbach's coefficient alpha, and yielded coefficients of 0,944, 0,736 and 0,803 respectively. The factor structure as conceptualised by Bass (1985 was largely confirmed in the present study. Opsomming Die doel van die studie was om vas te stel of die faktorstruktuur van die Veelfaktorleierskapsvraelys (MLQ van Bass, as maatstafvan transformasionele leierskap, in die Suid-Afrikaanse konteks gerepliseer kon word. Die MLQ is gekies omdat dit belofte inhou as n geldige en betroubare meetinstrument van die onderhawige konstruk, en ook weens die feit dat daar 'n dringende behoefte bestaan aan so n instrument vir gebruik in die bestuur van menslike hulpbronne in organisasies tydens verandering. Die MLQ is op 406 proefpersone binne militêre konteks toegepas, en aan n faktorontleding en n itemontleding onderwerp. Die faktorontleding het drie faktore opgelewer, te wete transformasionele leierskap, transaksionele leierskap en vermyding van leierskap ("laissez faire"-leierskap. Die betroubaarheid van die skale is bepaal

  8. Continuous Tidal Streamflow and Gage-Height Data for Bass and Cinder Creeks on Kiawah Island, South Carolina, September 2007

    Science.gov (United States)

    Conrads, Paul A.; Erbland, John W.

    2009-01-01

    A three-dimensional model of Bass and Cinder Creeks on Kiawah Island, South Carolina, was developed to evaluate methodologies for determining fecal coliform total maximum daily loads for shellfish waters. To calibrate the model, two index-velocity sites on the creeks were instrumented with continuous acoustic velocity meters and water-level sensors to compute a 21-day continuous record of tidal streamflows. In addition to monitoring tidal cycles, streamflow measurements were made at the index-velocity sites, and tidal-cycle streamflow measurements were made at the mouth of Bass Creek and on the Stono River to characterize the streamflow dynamics near the ocean boundary of the three-dimensional model at the beginning, September 6, 2007, and end, September 26, 2007, of the index-velocity meter deployment. The maximum floodtide and ebbtide measured on the Stono River by the mouth of Bass Creek for the two measurements were -155,000 and 170,000 cubic feet per second (ft3/s). At the mouth of Bass Creek, the maximum floodtide and ebbtide measurements during the 2 measurement days were +/-10,200 ft3/s. Tidal streamflows for the 21-day deployment on Bass Creek ranged from -2,510 ft3/s for an incoming tide to 4,360 ft3/s for an outgoing tide. On Cinder Creek, the incoming and outgoing tide varied from -2,180 to 2,400 ft3/s during the same period.

  9. Effect of different dose gamma radiation and refrigeration on the chemical and sensory properties and microbiological status of aqua cultured sea bass (Dicentrarchus labrax)

    International Nuclear Information System (INIS)

    Quality and shelf life of non-irradiated and irradiated (2.5 and 5kGy) sea bass in ice conditions and stored at +4 deg. C were investigated by measurement in microbiological, chemical sensory analyses. Microbial counts for non-irradiated sea bass samples were higher than irradiated fish. Among chemical indicators of spoilage, total volatile base nitrogen (TVB-N) values increased to 36.44mg/100g for non-irradiated sea bass during iced storage, whereas for irradiated fish lower values of 25.26mg/100g and 23.61mg/100g were recorded at 2.5 and 5kGy, respectively (day 17). Trimethylamine (TMA-N) values and thiobarbituric acid (TBA) values for irradiated samples were lower than that for non-irradiated samples. Acceptability scores for odour, taste and texture of cooked sea bass decreased with storage time. The sensory scores of sea bass stored in control and 2.5-5kGy at +4 deg. C were 13 and 15 days, respectively. The results obtained from this study showed that the shelf life of sea bass stored in ice, as determined by overall acceptability of all data, is 13 days for non-irradiated sea bass and 15 days for 2.5kGy irradiated and 17 days for 5kGy irradiated sea bass

  10. Control of Branchionus sp. and Amoeba sp. in cultures of Arthrospira sp. Control de Branchionus sp. y Amoeba sp. en cultivos de Arthrospira sp.

    OpenAIRE

    Carlos Méndez; Eduardo Uribe

    2012-01-01

    Cultivation of cyanobacterium Arthrospira sp. has been developed in many countries for the production of proteins, pigments and other compounds. Outdoor mass cultures are often affected by biological contamination, drastically reducing productivity as far as bringing death. This study evaluates the control of Branchionus sp. and Amoeba sp. with two chemical compounds: urea (U) and ammonium bicarbonate (AB), in laboratory conditions and outdoor mass culture of Arthrospira sp. The lethal concen...

  11. Varying plant protein sources in the diet of sea bass Dicentrarchus labrax differently affects lipid metabolism and deposition

    Directory of Open Access Journals (Sweden)

    E. Tibaldi

    2010-04-01

    Full Text Available The liver activity of lipogenic enzymes, the lipid content in various tissues, and plasma lipid levels of major, were measured in sea bass (D. labrax fed over 96 days either a, fish meal-based control diet or preparations where 70% of fish meal protein was replaced by wheat gluten singly or in combination with pea or soybean meals. Relative to the controls, sea bass fed the wheat gluten-based diet resulted in stimulated lipogenesis in liver and increased lipid deposition in muscle. The opposite occurred when a substantial amount of soybean meal was included in the diet. Mesenteric fat depots were apparently insensitive to major changes in dietary protein source in fish showing similar intakes of digestible protein, energy and lipid. These results confirm that varying plant protein source in the diet differently affects lipid metabolism and deposition in sea bass.

  12. Zooplankton variability and larval striped bass foraging: Evaluating potential match/mismatch regulation

    Science.gov (United States)

    Chick, J.H.; Van Den Avyle, M.J.

    1999-01-01

    We quantified temporal and spatial variability of zooplankton in three potential nursery sites (river, transition zone, lake) for larval striped bass (Morone saxatilis) in Lake Marion, South Carolina, during April and May 1993-1995. In two of three years, microzooplankton (rotifers and copepod nauplii) density was significantly greater in the lake site than in the river or transition zone. Macrozooplankton (>200 ??m) composition varied among the three sites in all years with adult copepods and cladocerans dominant at the lake, and juvenile Corbicula fluminea dominant at the river and transition zone. Laboratory feeding experiments, simulating both among-site (site treatments) and within-site (density treatments) variability, were conducted in 1995 to quantify the effects of the observed zooplankton variability on foraging success of larval striped bass. A greater proportion of larvae fed in the lake than in the river or transition-zone treatments across all density treatments: mean (x), 10x and 100x. Larvae also ingested significantly more dry mass of prey in the lake treatment in both the mean and 10x density treatments. Field zooplankton and laboratory feeding data suggest that both spatial and temporal variability of zooplankton influence larval striped bass foraging. Prey density levels that supported successful foraging in our feeding experiments occurred in the lake during late April and May in 1994 and 1995 but were never observed in the river or transition zone. Because the rivers flowing into Lake Marion are regulated, it may be possible to devise flow management schemes that facilitate larval transport to the lake and thereby increase the proportion of larvae matched to suitable prey resources.

  13. An empirical comparison of stock identification techniques applied to striped bass

    Science.gov (United States)

    Waldman, John R.; Richards, R. Anne; Schill, W. Bane; Wirgin, Isaac; Fabrizio, Mary C.

    1997-01-01

    Managers of migratory striped bass stocks that mix along the Atlantic coast of the USA require periodic estimates of the relative contributions of the individual stocks to coastal mixed- stock fisheries; however, to date, a standard approach has not been adopted. We compared the performances of alternative stock identification approaches, using samples taken from the same sets of fish. Reference (known) samples were collected from three Atlantic coast spawning systems: the Hudson River, Chesapeake Bay, and the Roanoke River. Striped bass of mixed-stock origin were collected from eastern Long Island, New York, and were used as test (unknown) samples. The approaches applied were discriminant analysis of morphometric data and of meristic data, logistic regression analysis of combined meristic and morphometric data, discriminant analysis of scale-shape features, discriminant analysis of immunoassay data, and mixed-stock analysis of mitochondrial DNA (mtDNA) data. Overall correct classification rates of reference samples ranged from 94% to 66% when just the Hudson and Chesapeake stocks were considered and were comparable when the Chesapeake and Roanoke stocks were grouped as the ''southern'' stock. When all three stocks were treated independently, correct classification rates ranged from 82% to 49%. Despite the moderate range in correct classification rates, bias due to misallocation was relatively low for all methods, suggesting that resulting stock composition estimates should be fairly accurate. However, relative contribution estimates for the mixed-stock sample varied widely (e.g., from 81% to 47% for the Hudson River stock, when only the Hudson River and Chesapeake Bay stocks were considered). Discrepancies may be related to the reliance by all of these approaches (except mtDNA) on phenotypic features. Our results support future use of either a morphometrics-based approach (among the phenotypic methods) or a genotypic approach based on mtDNA analysis. We further

  14. A large volume striped bass egg incubation chamber: design and comparison with a traditional method

    Science.gov (United States)

    Harper, C.J.

    2009-01-01

    I conducted a comparative study of a new jar design (experimental chamber) with a standard egg incubation vessel (McDonald jar). Experimental chambers measured 0.4 m in diameter by 1.3 m in height and had a volume of 200 L. McDonald hatching jars measured 16 cm in diameter by 45 cm in height and had a volume of 6 L. Post-hatch survival was estimated at 48, 96 and 144 h. Stocking rates resulted in an average egg density of 21.9 eggs ml-1 (range = 21.6 – 22.1) for McDonald jars and 10.9 eggs ml-1 (range = 7.0 – 16.8) for experimental chambers. I was unable to detect an effect of container type on survival to 48, 96 or 144 h. At 144 h striped bass fry survival averaged 37.3% for McDonald jars and 34.2% for experimental chambers. Survival among replicates was significantly different. Survival of striped bass significantly decreased between 96 and 144 h. Mean survival among replicates ranged from 12.4 to 57.3%. I was unable to detect an effect of initial stocking density on survival. Experimental jars allow for incubation of a larger number of eggs in a much smaller space. As hatchery production is often limited by space or water supply, experimental chambers offer an alternative to extending spawning activities, thereby reducing manpower and cost. However, the increase in the number of eggs per rearing container does increase the risk associated with catastrophic loss of a production unit. I conclude the experimental chamber is suitable for striped bass egg incubation.

  15. Evaluation of different methods of stunning/killing sea bass (Dicentrarchus labrax) by tissue stress/quality indicators

    OpenAIRE

    Zampacavallo, Giulia; Parisi, Giuliana; Mecatti, Massimo; Lupi, Paola; Giorgi, Gianluca; Poli, Bianca Maria

    2014-01-01

    The aim of the study was to evaluate the effect on the final product quality of certain innovative stunning/killing methods for sea bass as substitutes for the most common methods used by European farmers. The changes in tissue stress/quality parameters were monitored from the first hours after death and during the shelf life of the fish. Two trials were conducted in July and November on n. 231 sea bass stunned/killed by ice-water slurry, by single gas or mixture of gases in ice-water and by ...

  16. Diversity of estuarine movements of striped bass (Morone saxatilis): a synoptic examination of an estuarine system in southern New Jersey

    OpenAIRE

    Kenneth W. Able; Grothues, Thomas M.

    2007-01-01

    We determined the dis-tribution of multiple (n=68; 508−978 mm total length [TL]) striped bass (Morone saxatilis) along the estua-rine salinity gradient in the Mullica River−Great Bay in southern New Jersey over two years to determine the diversity of habitat use and the movements of striped bass. Ultrasoni-cally tagged fish were detected in this estuarine area by means of wireless hydrophones deployed at four gates inside the entrance of the study area and farther up to tidal freshwater (38 k...

  17. Growth parameters in European sea bass (Dicentrarchus labraxL.): effects of live weight and water temperature

    OpenAIRE

    Rodolfo Ballestrazzi; Edo D’Agaro; Domenico Lanari

    2010-01-01

    The voluntary feed intake (VFI) and growth rate of sea bass were studied for 147 days, based on different starting live  weights, under natural photoperiods and varied water temperatures. Sea bass (n = 720) were divided into five weight  classes (60-70, 90-110, 130-150, 160-180 and 230-250 g) and distributed among 20 tanks. Seven different water tem-  peratures were compared: 13, 16, 19, 22, 25, 28 and 31 °C. A commercial extruded diet (N x 6.25, 43.7% DM; crude  fat, ...

  18. Effects of herbal supplements on growth performance of sea bass (Dicentrarchus labrax: Change in body composition and some blood parameters

    Directory of Open Access Journals (Sweden)

    SEVDAN YILMAZ

    2012-01-01

    Full Text Available This study was conducted to investigate the effects of dietary thyme (Thymus vulgaris, rosemary (Rosmarinus officinalis and fenugreek (Trigonella foenum graecum as feed additives on growth performance, proximate composition and ammonia excretion of European sea bass Dicentrarchus labrax. Four isonitrogenous (48% crude protein and isocaloric (21 kj/g diets were formulated to contain 0% (control or 1% of thyme, rosemary or fenugreek. The thyme supplementation significantly increased protein efficiency ratio, fillet protein levels, protein and energy retentions (P0.05. The results indicate that dietary thyme improved the protein and energy retentions of sea bass.

  19. Biochemical and nutritional traits of sea bass (Dicentrachus labrax from different rearing systems.

    Directory of Open Access Journals (Sweden)

    Andrea Santulli

    2010-01-01

    Full Text Available Farmed European sea bass (Dicentrarchus labrax, sampled from three different culture systems (intensive in sea-cages, intensive in land-based basins and extensive in lagoon and storage basins of salt-work, of the Northern, Central and Southern Italy, were analyzed with the aim to employ nutritional trait to describe and to distinguish the “origin” of the product. Lipid and fatty acid profile, strongly affected by the feeding history and environmental factors, responsible of the nu- tritional and perceived quality of fish product, are proposed as marker of origin.

  20. IGF-I and branchial IGF receptor expression and localization during salinity acclimation in striped bass

    DEFF Research Database (Denmark)

    Tipsmark, Christian Kølbaek; Luckenbach, John Adam; Madsen, Steffen; Borski, Russell John

    2007-01-01

    The initial response of the IGF-I system and the expression and cellular localization of IGF type-I receptor (IGF-IR) were studied in the gill of a euryhaline teleost during salinity acclimation. Exposure of striped bass (Morone saxatilis) to hyperosmotic and hypoosmotic challenges induced small,...... first time in teleosts that IGF-IR and Na+-K+-ATPase are localized in putative chloride cells at the base of the lamellae, identifying these cells in the gill as a target for IGF-I and IGF-II. Overall the data suggest a hyperosmoregulatory role of IGF-I in this species....

  1. Betanodavirus ability to infect juvenile European sea bass, Dicentrarchus labrax, at different water salinity.

    Science.gov (United States)

    Pascoli, F; Serra, M; Toson, M; Pretto, T; Toffan, A

    2016-09-01

    Viral encephalopathy and retinopathy (VER) is one of the most devastating and economically relevant diseases for marine aquaculture. The presence of betanodavirus in freshwater fish is recorded, but very little is known about VER outbreaks in marine species reared in freshwater. Our study investigated the ability of betanodavirus to cause disease in European sea bass, Dicentrarchus labrax, reared at different salinity levels. Fish were challenged with RGNNV or mock infected by bath at different salinity levels (freshwater, 25‰ and 33‰). Fish were checked twice a day and the dead ones were examined by standard virological techniques, by rRT-PCR and by histochemical and immunohistochemical analyses. All the infected groups showed a significant higher mortality rate than the one of the mock-infected group. VERv presence was confirmed by rRT-PCR. Histochemical and immunohistochemical analyses highlighted the typical lesions associated with VER. Our results highlight that salinity does not affect the ability of betanodavirus to induce clinical signs and mortality in European sea bass infected under experimental conditions. These results underline the great adaptation potential of VERv, which in combination with its already known high environmental resistance and broad host range, may explain the diffusion of this disease and the threat posed to aquaculture worldwide. PMID:26763095

  2. The Bass diffusion model on networks with correlations and inhomogeneous advertising

    Science.gov (United States)

    Bertotti, M. L.; Brunner, J.; Modanese, G.

    2016-09-01

    The Bass model, which is an effective forecasting tool for innovation diffusion based on large collections of empirical data, assumes an homogeneous diffusion process. We introduce a network structure into this model and we investigate numerically the dynamics in the case of networks with link density $P(k)=c/k^\\gamma$, where $k=1, \\ldots , N$. The resulting curve of the total adoptions in time is qualitatively similar to the homogeneous Bass curve corresponding to a case with the same average number of connections. The peak of the adoptions, however, tends to occur earlier, particularly when $\\gamma$ and $N$ are large (i.e., when there are few hubs with a large maximum number of connections). Most interestingly, the adoption curve of the hubs anticipates the total adoption curve in a predictable way, with peak times which can be, for instance when $N=100$, between 10% and 60% of the total adoptions peak. This may allow to monitor the hubs for forecasting purposes. We also consider the case of networks with assortative and disassortative correlations and a case of inhomogeneous advertising where the publicity terms are "targeted" on the hubs while maintaining their total cost constant.

  3. Estimates of entrainment mortality for striped bass and other fish species inhabiting the Hudson River estuary

    International Nuclear Information System (INIS)

    An empirically derived age-, time-, and space-variant equation was used to estimate entrainment mortality at power plants for seven fish species inhabiting the Hudson River estuary. Entrainment mortality is expressed as a conditional rate, which is the fractional reduction in year-class strength due to entrainment if other sources of mortality are density-independent. Estimates of the conditional entrainment mortality, based on historical and projected once-through cooling operation of five power plants, were 11-22% for striped bass, 11-17% for white perch, 5-7% for Atlantic tomcod, 14-21% for American shad, 4-11% for river herring (alewife and blueback herring combined), and 35-79% for bay anchovy. Closed-cycle cooling (natural-draft cooling towers) at three of the power plants (Indian Point, Bowline Point, and Roseton) would reduce entrainment mortality of striped bass by 50-80%, of white perch by 75-80%, of Atlantic tocod by 65-70%, of American shad by 80%, of river herring by 30-90%, and of bay anchovy by 45-80%. The life stages most vulnerable to entrainment mortality were post-yolk-sac larva and entrainable size juvenile. 18 refs., 7 tabs

  4. BASS-Ultracool : A Survey for Isolated Analogs of Methane Exoplanets

    Science.gov (United States)

    Gagné, Jonathan; Faherty, Jacqueline K.; Malo, Lison; Filippazzo, Joseph C.; Burgasser, Adam J.; Artigau, Etienne; Lafrenière, David; Doyon, Rene; Bowsher, Emily; Nicholls, Christine P.

    2015-12-01

    I will present BASS-Ultracool, a new survey to identify isolated cold, late L and T-type members of young moving groups. These objects have masses below 10 MJup and physical properties similar to those of exoplanets identified with the direct-imaging method. The discovery of such isolated planetary-mass objects will allow us to characterize their atmospheres with unprecedented signal-to-noise and spectroscopic resolution due to the absence of a host star. They will serve as benchmarks to understand cold exoplanets such as the recently discovered 51 Eri b.I will also present how the prototype version of the BASS-Ultracool survey has already identified the first isolated T-type member of a nearby moving group SDSS J1110+0116, which is a young 10-12 MJup T5.5 member of the ~150 Myr-old AB Doradus moving group. This object is an isolated and slightly cooler version of the previously identified T3.5 AB Doradus member GU Psc b.

  5. Effects of the Presidente Rivera oil spill on young-of-year striped bass

    International Nuclear Information System (INIS)

    On 24, June 1989, approximately 300,000 gallons of No. 6 fuel oil was spilled from the tanker Presidente Rivera into the Delaware River. This paper reports that the spill occurred in the center of the striped bass nursery area, only six weeks after the prime spawning period. Toxic effects of the spill on young-of-year striped bass were investigated using in situ bioassay techniques. Seventy-five liter chambers, each containing 30 hatchery-reared fish, were moored at four locations within the spill zone and at one upstream references are. chemical analysis of the water was conducted on area. Chemical analysis of the water was conducted on Days 1, 4, and 13 of the experiment. Despite significant oil fouling on chambers, no dissolved aromatic hydrocarbons were detected in the water column. Acute mortality was not apparent, with greater than 90% survival at all stations after four days. After 13 days, survival at the station closest to the spill site was about 20% less than at the reference station

  6. Combustion of Solids in Microgravity: Results from the BASS-II Experiment

    Science.gov (United States)

    Ferkul, Paul V.; Bhattacharjee, Subrata; Fernandez-Pello, Carlos; Miller, Fletcher; Olson, Sandra L.; Takahashi, Fumiaki; T’ien, James S.

    2014-01-01

    The Burning and Suppression of Solids-II (BASS-II) experiment was performed on the International Space Station. Microgravity combustion tests burned thin and thick flat samples, acrylic slabs, spheres, and cylinders. The samples were mounted inside a small wind tunnel which could impose air flow speeds up to 53 cms. The wind tunnel was installed in the Microgravity Science Glovebox which supplied power, imaging, and a level of containment. The effects of air flow speed, fuel thickness, fuel preheating, and oxygen concentration on flame appearance, growth, spread rate, and extinction were examined in both the opposed and concurrent flow configuration. The flames are quite sensitive to air flow speed in the range 0 to 5 cms. They can be sustained at very low flow speeds of less than 1 cms, when they become dim blue and stable. In this state they are not particularly dangerous from a fire safety perspective, but they can flare up quickly with a sudden increase in air flow speed. Including earlier BASS-I results, well over one hundred tests have been conducted of the various samples in the different geometries, flow speeds, and oxygen concentrations. There are several important implications related to fundamental combustion research as well as spacecraft fire safety. This work was supported by the NASA Space Life and Physical Sciences Research and Applications Division (SLPSRA).

  7. Age-at-maturity estimates for Atlantic coast female striped bass

    Science.gov (United States)

    Berlinsky, David L.; Fabrizio, Mary C.; O'Brien, John F.; Specker, Jennifer L.

    1995-01-01

    This study was undertaken to estimate the percentage of mature female striped bass Morone saxatilis present in each age-class during annual coastal feeding migration. Migratory striped bass (N = 302) were sampled in coastal Rhode Island waters during spring (May-June) and fall (September-November) from 1985 to 1987. Stocks were identified by analysis of morphometric characters and isoelectric focusing of eye-lens proteins. Histological sections of ovarian tissue were used to categorize maturity state. Fish were considered mature if a class of oocytes measuring at least 150 μm and containing cytoplasmic inclusions was found in the ovarian sections. All females whose age at next potential spawning was 7 and older were mature. Our empirical observations indicated that 12% of fish in age-class 4, 34% of fish in age-class 5, and 77% of fish in age-class 6 were mature. The estimate of the proportion of mature fish in age-class 5 differs significantly from that of Merriman (1941), who also examined coastal migrants. No significant differences were found in maturity estimates of fish from stocks of different origin.

  8. Implications of power plant mortality for management of the Hudson River striped bass fishery

    International Nuclear Information System (INIS)

    The Atlantic coastal stock of striped bass apparently declined from colonial times to the early 1930s and subsequently recovered. The reasons for the decline and recovery are not known, but fishing remains a possible explanation, which would suggest population sensitivity to increased mortality. Evidence suggests that fishing mortality has been increasing in recent years and will continue to increase in the absence of management intervention. The consequence of increased fishing mortality is an increase in the marginal effect of the power plant mortality which based on the utilities' models and parameter fits, could result in important reductions in the Hudson River striped bass population. Any management actions imposed to arrest population decline or to increase yield per effort in the fishery would be required to mitigate the impact of the power plants by reducing fishing mortality. It is estimated that a 20% conditional power plant mortality is equivalent to a 14% increase in the number of average fishermen using the stock. Consequently, should any management intervention be required on behalf of the population, managers would be required to reduce fishing mortality by about 14% just to account for the power plant mortality. 26 refs., 5 figs., 4 tabs

  9. Abundance and distribution of northern squawfish, walleyes, and smallmouth bass in John Day Reservoir, Columbia river

    International Nuclear Information System (INIS)

    The authors used mark-recapture and catch-per-unit effort data to estimate abundances and distributions of three potential predators on juvenile salmonids migrating through John Day Reservoir in 1984-1986. The northern squawfish Ptychocheilus oregonensis was the most abundant predator (estimated population: 85, 316), followed by smallmouth bass Micropterus dolomieu (34,954) and walleye Stizostedion vitreum (15,168). Because of uncertainty in sampling and assumption of the mark-recapture estimator, the combined abundance of these three predators could lie between 50,000 and 500,000. They believe, however, that bias is probably negative, and that any errors should result in conservative estimates. Northern squawfish were common reservoir-wide, but large concentrations occurred immediately below McNary Dam near the head of John Day Reservoir. Walleyes were largely restricted to the upper third of the reservoir, whereas the number of smallmouth bass increased progressively downriver. As judged by abundance and distribution, northern squawfish have by far the greatest potential for predation on juvenile salmonids. They also expect predation to be unevenly distributed in time and space as a result of variations in the number and distribution of predators

  10. Real Option Approach to Economic Analysis of European Sea Bass (Dicetrarchus labrax Farming in Croatia

    Directory of Open Access Journals (Sweden)

    Lari Hadelan

    2012-10-01

    Full Text Available The paper emphasizes the economic performance of the fresh European sea bass production and profitability of related processing to value-added fillets. Croatian annual farmed European sea bass and gilthead sea bream production in amount of 4,000 tones plays only about 1.7% of the World production with mediocre economic benefits for producers. However, product diversification, including processing measures as filleting, vacuuming and smoked processing can ensure additional product value providing long-term strategic orientation for fish-farmers. Seabass filleting, although at the initial phase, can be a modus of value-added production, which protects the producers of the price risk volatility targeting the population averse to the long lasting traditional fish mill preparation. Applied Real Option method can be helpful tool in the situation when the strategic project value includes not just the current economic features but also opportunities related to the basic model. Option approach indicates that seabass filleting triplicate the economic performance with respect to fresh seabass production.

  11. Competitive interactions between walleye (Sander vitreus) and smallmouth bass (Micropterus dolomieu) under various controlled conditions

    Science.gov (United States)

    Wuellner, M.R.; Graeb, B.D.S.; Willis, D.W.; Galster, B.J.; Selch, T.M.; Chipps, S.R.

    2011-01-01

    The range of smallmouth bass (Micropterus dolomieu) is expanding northward, creating new interactions with native predators, including walleye (Sander vitreus). We used a series of experiments to investigate competition between walleye (WAE) and smallmouth bass (SMB) at different life stages and light conditions, identified behaviors that allowed one fish to outcompete another, and evaluated whether prey switching mitigated competitive interactions. Juvenile and adult SMB appeared to outcompete WAE when fed during the daytime; neither species dominated when fed near dusk. Attack rates and capture efficiencies of both species were similar with an intra- or interspecific competitor, but SMB often exploited prey before the competitor had a chance to feed (exploitative competition) or displayed agonistic behaviors toward a potential competitor (interference competition). Prey selectivity of WAE or SMB did not differ when by themselves or with a potential competitor. These results indicate that SMB could outcompete WAE under limiting prey conditions due to the aggressive nature of SMB, but resources may be partitioned at least along a temporal scale. ?? 2011 Taylor & Francis.

  12. The Bass diffusion model on networks with correlations and inhomogeneous advertising

    CERN Document Server

    Bertotti, M L; Modanese, G

    2016-01-01

    The Bass model, which is an effective forecasting tool for innovation diffusion based on large collections of empirical data, assumes an homogeneous diffusion process. We introduce a network structure into this model and we investigate numerically the dynamics in the case of networks with link density $P(k)=c/k^\\gamma$, where $k=1, \\ldots , N$. The resulting curve of the total adoptions in time is qualitatively similar to the homogeneous Bass curve corresponding to a case with the same average number of connections. The peak of the adoptions, however, tends to occur earlier, particularly when $\\gamma$ and $N$ are large (i.e., when there are few hubs with a large maximum number of connections). Most interestingly, the adoption curve of the hubs anticipates the total adoption curve in a predictable way, with peak times which can be, for instance when $N=100$, between 10% and 60% of the total adoptions peak. This may allow to monitor the hubs for forecasting purposes. We also consider the case of networks with a...

  13. Evidence of estrogenic endocrine disruption in smallmouth and largemouth bass inhabiting Northeast U.S. National Wildlife Refuge waters: A reconnaissance study

    Science.gov (United States)

    Iwanowicz, Luke; Blazer, Vicki; Pinkney, A.E.; Guy, C.P.; Major, A.M.; Munney, K.; Mierzykowski, S.; Lingenfelser, S.; Secord, A.; Patnode, K.; Kubiak, T.J.; Stern, C.; Hahn, Cassidy M.; Iwanowicz, Deborah; Walsh, Heather L.; Sperry, Adam J.

    2016-01-01

    Intersex as the manifestation of testicular oocytes (TO) in male gonochoristic fishes has been used as an indicator of estrogenic exposure. Here we evaluated largemouth bass (Micropterus salmoides) or smallmouth bass (Micropterus dolomieu) form 19 National Wildlife Refuges (NWRs) in the Northeast U.S. inhabiting waters on or near NWR lands for evidence of estrogenic endocrine disruption. Waterbodies sampled included rivers, lakes, impoundments, ponds, and reservoirs. Here we focus on evidence of endocrine disruption in male bass evidenced by gonad histopathology including intersex or abnormal plasma vitellogenin (Vtg) concentrations. During the fall seasons of 2008–2010, we collected male smallmouth bass (n=118) from 12 sites and largemouth bass (n=173) from 27 sites. Intersex in male smallmouth bass was observed at all sites and ranged from 60% to 100%; in male largemouth bass the range was 0–100%. Estrogenicity, as measured using a bioluminescent yeast reporter, was detected above the probable no effects concentration (0.73 ng/L) in ambient water samples from 79% of the NWR sites. Additionally, the presence of androgen receptor and glucocorticoid receptor ligands were noted as measured via novel nuclear receptor translocation assays. Mean plasma Vtg was elevated (>0.2 mg/ml) in male smallmouth bass at four sites and in male largemouth bass at one site. This is the first reconnaissance survey of this scope conducted on US National Wildlife Refuges. The baseline data collected here provide a necessary benchmark for future monitoring and justify more comprehensive NWR-specific studies.

  14. Evidence of estrogenic endocrine disruption in smallmouth and largemouth bass inhabiting Northeast U.S. national wildlife refuge waters: A reconnaissance study.

    Science.gov (United States)

    Iwanowicz, L R; Blazer, V S; Pinkney, A E; Guy, C P; Major, A M; Munney, K; Mierzykowski, S; Lingenfelser, S; Secord, A; Patnode, K; Kubiak, T J; Stern, C; Hahn, C M; Iwanowicz, D D; Walsh, H L; Sperry, A

    2016-02-01

    Intersex as the manifestation of testicular oocytes (TO) in male gonochoristic fishes has been used as an indicator of estrogenic exposure. Here we evaluated largemouth bass (Micropterus salmoides) or smallmouth bass (Micropterus dolomieu) form 19 National Wildlife Refuges (NWRs) in the Northeast U.S. inhabiting waters on or near NWR lands for evidence of estrogenic endocrine disruption. Waterbodies sampled included rivers, lakes, impoundments, ponds, and reservoirs. Here we focus on evidence of endocrine disruption in male bass evidenced by gonad histopathology including intersex or abnormal plasma vitellogenin (Vtg) concentrations. During the fall seasons of 2008-2010, we collected male smallmouth bass (n=118) from 12 sites and largemouth bass (n=173) from 27 sites. Intersex in male smallmouth bass was observed at all sites and ranged from 60% to 100%; in male largemouth bass the range was 0-100%. Estrogenicity, as measured using a bioluminescent yeast reporter, was detected above the probable no effects concentration (0.73ng/L) in ambient water samples from 79% of the NWR sites. Additionally, the presence of androgen receptor and glucocorticoid receptor ligands were noted as measured via novel nuclear receptor translocation assays. Mean plasma Vtg was elevated (>0.2mg/ml) in male smallmouth bass at four sites and in male largemouth bass at one site. This is the first reconnaissance survey of this scope conducted on US National Wildlife Refuges. The baseline data collected here provide a necessary benchmark for future monitoring and justify more comprehensive NWR-specific studies. PMID:26454754

  15. Hypoxia affects performance traits and body composition of juvenile hybrid striped bass (Morone chrysops x M. saxatilis)

    Science.gov (United States)

    Performance traits and body composition of juvenile hybrid striped bass (Morone chrysops x M. saxatilis) in response to hypoxia were evaluated in replicate tanks maintained at constant dissolved oxygen concentrations that averaged 23.0 +/- 2.3%, 39.7 +/- 3.0%, and 105.5 +/- 9.5% dissolved oxygen sat...

  16. Quantitative genetics and differential performance and gene expression of half-sib families of hybrid striped bass in communal ponds

    Science.gov (United States)

    The US is one of the world’s largest importers of seafood. A major constraint in producing hybrid striped bass is suboptimal production efficiency due to large performance variation of fish from undomesticated brooders. The objectives of this first-year study were to determine the genetic basis of p...

  17. Minimizing use of fish meal in sunshine bass diets using standard and new varieties of non-genetically modified soybeans

    Science.gov (United States)

    Improved plant ingredients are needed to support sustainable culture of carnivorous fish, such as hybrid striped bass (HSB). We are evaluating meals made from new strains of non-genetically-modified soybeans (non-GMO) with high protein and reduced anti-nutritional factors (ANFs) on HSB nutrient dige...

  18. Computer simulation model for the striped bass young-of-the-year population in the Hudson River

    International Nuclear Information System (INIS)

    This report presents a daily transient (tidal-averaged), longitudinally one-dimensional (cross-section-averaged) computer simulation model for the assessment of the entrainment and impingement impacts of power plant operations on young-of-the-year populations of the striped bass, Morone saxatilis, in the Hudson River

  19. Phenanthrene and nitrite effects on juvenile sea bass, Dicentrarchus labrax, using hepatic biotransformation enzymes, biliary fluorescence, and micronuclei as biomarkers

    Digital Repository Service at National Institute of Oceanography (India)

    Reis-Henriques, M.A.; Ferreira, M.; Coimbra, A.M.; DeSilva, C.; Shailaja, M.S.

    measured at days 1, 3, and 6 of exposure. Sea bass exposed to PHE exhibited lower hepatic 7-ethoxyresorufin O-deethylase (EROD) activity as compared to the control group. The activity of the phase II enzyme, glutathione S-transferase (GST), was similar...

  20. Survival of vaccinated,feed-trained largemouth bass fry (Micropterus Salmoides Floridanus) during natural exposure to Flavobacterium columnare

    Science.gov (United States)

    Largemouth bass (Micropterus salmoides floridanus; Centrarchidae) are often reared in government hatchery programs, then stocked to supplement wild fish populations. After the eggs obtained from broodstock are hatched, fry are stocked into ponds to feed on zooplankton and other small invertebrates....

  1. 77 FR 76950 - Fisheries of the Northeastern United States; Summer Flounder, Scup, and Black Sea Bass Fisheries...

    Science.gov (United States)

    2012-12-31

    ... 23, 2012 (77 FR 24151). An error was found in the summer flounder commercial quota and recreational... Register of April 23, 2012, in FR Doc. 2012-9755, on page 24152, Table 1 is corrected as follows: Table 1... Northeastern United States; Summer Flounder, Scup, and Black Sea Bass Fisheries; 2012 Summer Flounder,...

  2. Controlled Acoustic Bass System (CABS) A Method to Achieve Uniform Sound Field Distribution at Low Frequencies in Rectangular Rooms

    DEFF Research Database (Denmark)

    Celestinos, Adrian; Nielsen, Sofus Birkedal

    2008-01-01

    The sound field produced by loudspeakers at low frequencies in small- and medium-size rectangular listening rooms is highly nonuniform due to the multiple reflections and diffractions of sound on the walls and different objects in the room. A new method, called controlled acoustic bass system (CABS...

  3. Parasitismo por Giardia sp. e Cryptosporidium sp. em Coendou villosus Parasitism by Giardia sp. and Cryptosporidium sp. in Coendou villosus

    Directory of Open Access Journals (Sweden)

    João Fabio Soares

    2008-04-01

    Full Text Available O objetivo deste trabalho foi verificar o possível parasitismo por Giardia sp. e Cryptosporidium sp. em amostras de fezes de ouriço-cacheiro (Coendou villosus. As amostras foram analisadas pelo método de centrífugo-flutuação com sulfato de zinco e apresentaram elevada infecção por cistos de Giardia sp. e por oocistos de Cryptosporidium sp., embora os animais não apresentassem sinal clínico decorrente disso.This research was aimed at verifing the possible parasitism by Giardia sp. and Cryptosporidium sp. in porcupine (Coendou villosus faeces samples. Samples were analyzed by the centrifugal-flotation method with zinc sulphate and showed high infection by cysts of Giardia sp. and by oocysts of Cryptosporidium sp., although the animals did not show any associated clinical sign.

  4. Comparison of denitrification between Paracoccus sp. and Diaphorobacter sp.

    Science.gov (United States)

    Chakravarthy, Srinandan S; Pande, Samay; Kapoor, Ashish; Nerurkar, Anuradha S

    2011-09-01

    Denitrification was compared between Paracoccus sp. and Diaphorobacter sp. in this study, both of which were isolated from activated sludge of a denitrifying reactor. Denitrification of both isolates showed contrasting patterns, where Diaphorobacter sp. showed accumulation of nitrite in the medium while Paracoccus sp. showed no accumulation. The nitrate reduction rate was 1.5 times more than the nitrite reduction in Diaphorobacter sp., as analyzed by the resting state denitrification kinetics. Increasing the nitrate concentration in the medium increased the nitrite accumulation in Diaphorobacter sp., but not in Paracoccus sp., indicating a branched electron transfer during denitrification. Diaphorobacter sp. was unable to denitrify efficiently at high nitrate concentrations from 1 M, but Paracoccus sp. could denitrify even up to 2 M nitrate. Paracoccus sp. was found to be an efficient denitrifier with insignificant amounts of nitrite accumulation, and it could also denitrify high amounts of nitrate up to 2 M. Efficient denitrification without accumulation of intermediates like nitrite is desirable in the removal of high nitrates from wastewaters. Paracoccus sp. is shown to suffice this demand and could be a potential organism to remove high nitrates effectively. PMID:21509603

  5. Behavioural thermoregulation of largemouth bass (Micropterus salmoides): response of naive fish to the thermal gradient in a nuclear reactor cooling reservoir

    International Nuclear Information System (INIS)

    1.1. We studied patterns of temperature selection of naive largemouth bass (Micropterus salmoides) transplanted from an adjacent normothermic site to a cooling reservoir for a nuclear reactor Lethally hot (> 45°C) water entered the reservoir periodically.2.2. Temperature-sensing radio transmitters, which were surgically implanted in 10 fish, enabled us to monitor movement of bass during different times of year in the thermally heterogeneous environment.3.3. Naive bass exhibited a range of reactive thermoregulatory behaviours which led half of them to cool-water refuges in the reservoir. Moreover, some bass appeared to learn the thermal characteristics of the refuges.4.4. These behaviours were sufficient for 30% of the naive fish to survive extreme thermal conditions

  6. Individual fish rhythm directs group feeding: a case study with sea bass juveniles (Dicentrarchus labrax) under self-demand feeding conditions

    OpenAIRE

    Millot, Sandie; Begout, Marie-laure

    2009-01-01

    The long term influence of individual biological rhythms on group feed demand behaviour was investigated in European sea bass (Dicentrarchus labrax) held under controlled environmental conditions with an on-demand feeding system. The experiment was realized over 219 days with 190 fish distributed on 4 tanks. Sea bass had a mean body mass comprised between 139 g to 183 g. The number of feed demand acts by each individual was calculated daily, and the population could thus be partitioned into t...

  7. LAS CLAVES DE LA INNOVACIÓN DE SAUL BASS EN LA SECUENCIA DE TÍTULOS DE CRÉDITO DE VÉRTIGO

    OpenAIRE

    Alcaraz Pagán, Mª De Las Nieves

    2016-01-01

    [EN] Saul Bass is considered as the father of contemporary title sequences. The main contribution of this work consists in verifying the close correspondences between Bass' deviced iconography, figures and visual motifs to summarize and synthesise the profound sense of the story told by the film, preceded by their sequences, since it is this idea that is found as the base of what has been established as the turning point and conceptual axis around his innovation. This is something until nowad...

  8. An ovary transcriptome for all maturational stages of the striped bass (Morone saxatilis), a highly advanced perciform fish

    OpenAIRE

    Reading Benjamin J; Chapman Robert W; Schaff Jennifer E; Scholl Elizabeth H; Opperman Charles H; Sullivan Craig V

    2012-01-01

    Abstract Background The striped bass and its relatives (genus Morone) are important fisheries and aquaculture species native to estuaries and rivers of the Atlantic coast and Gulf of Mexico in North America. To open avenues of gene expression research on reproduction and breeding of striped bass, we generated a collection of expressed sequence tags (ESTs) from a complementary DNA (cDNA) library representative of their ovarian transcriptome. Results Sequences of a total of 230,151 ESTs (51,259...

  9. Edward W. Taylor / Patricia Cranton, and Associates (Hrsg.): The Handbook of Transformative Learning, Theory, Research, and Practice. San Francisco, CA: Jossey-Bass 2012 [...] [Sammelrezension

    OpenAIRE

    Fuhr, Thomas

    2012-01-01

    Sammelrezension von: 1. Edward W. Taylor / Patricia Cranton, and Associates (Hrsg.): The Handbook of Transformative Learning, Theory, Research, and Practice, San Francisco, CA: Jossey-Bass 2012 (598 S.; ISBN 978-1-111-21891-4) 2. Jack Mezirow / Edward W. Taylor, and Associates (Hrsg.): Transformative Learning in Practice, Insights from Community, Workplace, and Higher Education, San Francisco, CA: Jossey-Bass 2009 (303 S.; ISBN 978-0-470-25790-6)

  10. Concurrent environmental stressors and jellyfish stings impair caged European sea bass (Dicentrarchus labrax) physiological performances.

    Science.gov (United States)

    Bosch-Belmar, Mar; Giomi, Folco; Rinaldi, Alessandro; Mandich, Alberta; Fuentes, Verónica; Mirto, Simone; Sarà, Gianluca; Piraino, Stefano

    2016-01-01

    The increasing frequency of jellyfish outbreaks in coastal areas has led to multiple ecological and socio-economic issues, including mass mortalities of farmed fish. We investigated the sensitivity of the European sea bass (Dicentrarchus labrax), a widely cultured fish in the Mediterranean Sea, to the combined stressors of temperature, hypoxia and stings from the jellyfish Pelagia noctiluca, through measurement of oxygen consumption rates (MO2), critical oxygen levels (PO2crit), and histological analysis of tissue damage. Higher levels of MO2, PO2crit and gill damage in treated fish demonstrated that the synergy of environmental and biotic stressors dramatically impair farmed fish metabolic performances and increase their health vulnerability. As a corollary, in the current scenario of ocean warming, these findings suggest that the combined effects of recurrent hypoxic events and jellyfish blooms in coastal areas might also threaten wild fish populations. PMID:27301314

  11. Diffusion Raman basse fréquence dans les fibres optiques

    OpenAIRE

    Saissy, A.

    1986-01-01

    Nous comparons la diffusion Raman basse fréquence,10 à 100 cm-1, de fibres optiques constituées de divers matériaux à structure désordonnée (silice, verre fluoré, polymère). Les propriétés propagatives des fibres pour les ondes lumineuses et acoustiques sont prises en compte dans un modèle proposé par A. J. Martin et W. Brenig. Une longueur de corrélation structurale est déterminée pour chacun des matériaux : (6,8 ± 0,3) Å pour SiO2, (6,4 ± 0,5) Å pour ZrF4-BaF2 et (8,3 ± 1) Å pour PMMA. L'im...

  12. Seasonal variations of the humoral immune parameters of European sea bass (Dicentrarchus labrax L.).

    Science.gov (United States)

    Valero, Yulema; García-Alcázar, Alicia; Esteban, M Ángeles; Cuesta, Alberto; Chaves-Pozo, Elena

    2014-08-01

    Seasonal cycles, mainly due to great variations in the light duration and temperature, are important and modulate several aspects of the animal behavior. In the case of poikilotherms animals such as fish this is very relevant. Thus, temperature changes fish immunity and affects disease resistance. We evaluate in this work the season variations of the European sea bass (Dicentrarchus labrax) humoral innate parameters focusing on winter months, at which the culture of this specie is more difficult. Our results showed that not all the innate immune parameters are depressed by low temperatures. Moreover, some of them are more dependent than others to the season and both temperature and photoperiod are operating together. PMID:24852342

  13. Maison forte du Boisset : panoramique complet de la basse-cour

    OpenAIRE

    Pouyllau, Stéphane

    1996-01-01

    Panoramique de 360° réalisé par superposition d'images de la basse cour de la maison forte du boisset en 1998. Le Boisset est une maison forte reconstruite au XVe siècle (entre 1438 et 1480). Le site est entouré d'un anneau fossoyé. Certaines structures (au dessous du bâti actuel) et les fossés datent probablement de la fin du XIIIe siècle. Le premier site est à mettre en liaison avec la présence d'un lignage repérable dans ce secteur en 1260 : la famille des Brun de Boisset. Cette famille es...

  14. Bioenergetics estimate of the effects of stocking density on hatchery production of smallmouth bass fingerlings

    Science.gov (United States)

    Robel, G.L.; Fisher, W.L.

    1999-01-01

    Production of and consumption by hatchery-reared tingerling (age-0) smallmouth bass Micropterus dolomieu at various simulated stocking densities were estimated with a bioenergetics model. Fish growth rates and pond water temperatures during the 1996 growing season at two hatcheries in Oklahoma were used in the model. Fish growth and simulated consumption and production differed greatly between the two hatcheries, probably because of differences in pond fertilization and mortality rates. Our results suggest that appropriate stocking density depends largely on prey availability as affected by pond fertilization and on fingerling mortality rates. The bioenergetics model provided a useful tool for estimating production at various stocking density rates. However, verification of physiological parameters for age-0 fish of hatchery-reared species is needed.

  15. The effect of lordosis severity on juvenile sea bass (Dicentrarchus labrax L., 1758) swimming performance

    OpenAIRE

    Başaran, Fatih; ÖZBİLGİN, Hüseyin; ÖZBİLGİN, Yeliz DOĞANYILMAZ

    2009-01-01

    The relationship between the lordosis severity and swimming performance in sea bass juveniles was investigated. In total, 149 experimental animals 110-130 days old that ranged from 1.44 to 3.44 g (meanTW ± SD: 2.41 ± 0.49 g) and 5.4 to 6.5 cm (meanTL ± SD: 6.03 ± 0.39 cm) were used in experiments to determine critical swimming speed (Ucrit) in water that was 14 ± 1 °C. Vertebral angle in normal healthy fish is 180°. The lordosis severity in abnormal fish is indicated by a reduction in this va...

  16. A strain of Siniperca chuatsi rhabdovirus causes high mortality among cultured Largemouth Bass in South China.

    Science.gov (United States)

    Ma, Dongmei; Deng, Guocheng; Bai, Junjie; Li, Shengjie; Yu, Lingyun; Quan, Yingchun; Yang, Xiaojing; Jiang, Xiaoyan; Zhu, Zemin; Ye, Xing

    2013-09-01

    In April 2011, 40% mortality of Largemouth Bass Micropterus salmoides juveniles occurred at a farm of Zhongshan City, Guangdong Province, China. Infected fish became lethargic, exhibited corkscrew and irregular swimming, and developed a distended abdomen and crooked body. Fish began to die within 2 d after the appearance of clinical signs. In order to analyze the pathogeny and diagnose the disease earlier, observation of clinical signs, cell infection, titer calculation, electron microscopy, immersion infection assay for fish, and nucleotide sequence analysis were carried out. Fathead minnow (FHM) cell cultures, inoculated with filtrate of liver and spleen homogenates from the diseased fish, developed the obvious cytopathic effect 46 h after inoculation in the primary culture and 24 h at the first passage. Typical rhabdovirus particles, 115-143 nm in length and 62-78 nm in diameter, were observed in infected FHM cells by direct transmission electron microscopy. The isolated virus produced a titer of 10(7.15) TCID50/mL. Immersion-Fish infected with the virus had similar clinical signs and 80% mortality with 10(2.5) LD50/mL. The data indicated that the rhabdovirus was the lethal pathogeny of the current disease. Based on nucleoprotein-gene nucleotide sequence multiple alignment analysis, the newly isolated virus is a strain of Siniperca chuatsi rhabdovirus (SCRV) under family Rhabdoviridae, which was initially isolated from Mandarin Fish Siniperca chuatsi. Up to the present, at least four virus strains have been isolated from diseased Largemouth Bass, which have had different clinical signs. Comparison of the clinical signs can help in an early diagnosis of the disease. PMID:23915177

  17. Lipid traits and dietary quality of sea bass fillets from Orbetello

    Directory of Open Access Journals (Sweden)

    A. Bonelli

    2010-04-01

    Full Text Available The aim of the study was to investigate the possibility of obtaining: a reliable estimates of sea bass fillet weight and lipid/fatty acid content from simple non destructive morphometric measurements; b estimates of healthy eating quality evaluation from effects of consumption on blood biomarkers related to atherosclerotic/thrombogenic processes. A series of parameters were measured in 708 fish. Estimates of fillet weight from b.w. and max. thickness (R2=0.96, rsd=±20.55g and of lipid content from b.w., total length, max. thickness and condition factor (R2= 0.69, rsd=±3.3g were found to be very reliable; flesh n-3 PUFA and EPA content were also estimated from condition factor and max. girth (R2=0.32, rsd=±0.42g; R2=0.38, rsd=±0.13g. Flesh healthy eating quality was evaluated from changes in blood biomarkers (lipid profile, inflammatory markers, haemorheological profile in dyslipidemic subjects who had eaten fillets three times a week for 10 weeks. A weekly flesh intake of 807g increased erythrocyte n-3 PUFA (P<0.05 and decreased triglycerides (P<0.008, interleukin-6 (P<0.03, interleukin-8 (P<0.04, Whole Blood Viscosity 20.400 (P<0.04 and erythrocyte filtration (P<0.008. Short term intake of sea bass therefore seemed associated with favourable biochemical changes in dyslipidemic subjects, as reflected by lower circulating levels of markers of atherosclerosis.

  18. Behavioural stress responses predict environmental perception in European sea bass (Dicentrarchus labrax.

    Directory of Open Access Journals (Sweden)

    Sandie Millot

    Full Text Available Individual variation in the response to environmental challenges depends partly on innate reaction norms, partly on experience-based cognitive/emotional evaluations that individuals make of the situation. The goal of this study was to investigate whether pre-existing differences in behaviour predict the outcome of such assessment of environmental cues, using a conditioned place preference/avoidance (CPP/CPA paradigm. A comparative vertebrate model (European sea bass, Dicentrarchus labrax was used, and ninety juvenile individuals were initially screened for behavioural reactivity using a net restraining test. Thereafter each individual was tested in a choice tank using net chasing as aversive stimulus or exposure to familiar conspecifics as appetitive stimulus in the preferred or non preferred side respectively (called hereafter stimulation side. Locomotor behaviour (i.e. time spent, distance travelled and swimming speed in each tank side of each individual was recorded and analysed with video software. The results showed that fish which were previously exposed to appetitive stimulus increased significantly the time spent on the stimulation side, while aversive stimulus led to a strong decrease in time spent on the stimulation side. Moreover, this study showed clearly that proactive fish were characterised by a stronger preference for the social stimulus and when placed in a putative aversive environment showed a lower physiological stress responses than reactive fish. In conclusion, this study showed for the first time in sea bass, that the CPP/CPA paradigm can be used to assess the valence (positive vs. negative that fish attribute to different stimuli and that individual behavioural traits is predictive of how stimuli are perceived and thus of the magnitude of preference or avoidance behaviour.

  19. Quantitative immunoenzymatic detection of viral encephalopathy and retinopathy virus (betanodavirus) in sea bass Dicentrarchus labrax.

    Science.gov (United States)

    Nuñez-Ortiz, N; Stocchi, V; Toffan, A; Pascoli, F; Sood, N; Buonocore, F; Picchietti, S; Papeschi, C; Taddei, A R; Thompson, K D; Scapigliati, G

    2016-07-01

    Viral encephalopathy and retinopathy disease caused by betanodavirus, genus of the family Nodaviridae, affects marine, wild and farmed species including sea bass, one of the most important farmed species in Europe. This work describes a reliable and sensitive indirect ELISA assay to detect betanodavirus in biological samples using a polyclonal antiserum (pAb 283) against the 283/I09 virus strain, the most common red-spotted grouper nervous necrosis virus (RGNNV) genotype in the Mediterranean area, and a capture-based ELISA using a monoclonal antibody (mAb 4C3) specific to a common epitope present on the capsid protein. Using adsorbed, purified VERv preparation, the detection limit of indirect ELISA was 2 μg mL(-1) (3 × 10(5) TCID50 per mL), whereas for capture-based ELISA, the sensitivity for the antigen in solution was 17 μg mL(-1) (35 × 10(5) TCID50 per mL). The capture-based ELISA was employed to detect VERv in brain homogenates of in vivo infected sea bass and resulted positive in 22 of 32 samples, some of these with a high viral load estimates (about 1.1 × 10(8)  TCID50 per mL). The ELISA system we propose may be helpful in investigations where coupling of viral content in fish tissues with the presence of circulating VERv-specific IgM is required, or for use in samples where PCR is difficult to perform. PMID:26610431

  20. Acute toxicity of an acid mine drainage mixing zone to juvenile bluegill and largemouth bass

    Science.gov (United States)

    Henry, T.B.; Irwin, E.R.; Grizzle, J.M.; Wildhaber, M.L.; Brumbaugh, W.G.

    1999-01-01

    The toxicity of an acid mixing zone produced at the confluence of a stream that was contaminated by acid mine drainage (AMD) and a pH-neutral stream was investigated in toxicity tests with juvenile bluegill Lepomis macrochirus and largemouth bass Micropterus salmoides. Fish mortalities in instream cages located in the mixing zone, below the mixing zone, and upstream in both tributaries were compared to determine relative toxicity at each site. In all tests and for both species, significantly higher mortality was observed in the mixing zone than at any other location, including the acid stream, which had lower pH (2.9-4.3). The mixing zone was defined chemically by rapid precipitation of dissolved aluminum and iron, which arrived from the low-pH stream, and by the presence of white precipitates, which were attached to the substratum and which extended below the confluence. Possible seasonal changes in mixing zone toxicity were investigated by conducting field tests with bluegill in June, July, and August 1996 and in January 1997 and by conducting field tests with largemouth bass in April and May 1997. Toxicity was not significantly different at the extremes of temperature, pH, and metal concentration that occurred in June and July, as compared with January. Toxicity was significantly lower in August; however, elevated stream discharge during the August test may have disturbed mixing zone characteristics. High toxicity in AMD mixing zones may lower the survival of fishes in streams, reduce available habitat, and impede movements of migratory fish.

  1. Evaluation of potentially nonlethal sampling methods for monitoring mercury concentrations in smallmouth bass (Micropterus dolomieu)

    Science.gov (United States)

    Schmitt, C.J.; Brumbaugh, W.G.

    2007-01-01

    We evaluated three potentially nonlethal alternatives to fillet sampling for the determination of mercury (Hg) concentrations in smallmouth bass (Micropterus dolomieu). Fish (n = 62, 226-464 mm total length) from six sites in southern Missouri were captured by electrofishing. Blood samples (1 mL) from each fish were obtained by caudal veinipuncture with a heparinized needle and syringe. Biopsy needle (10 mm x 14 gauge; three cuts per fish; 10-20 mg total dry weight) and biopsy punch (7 mm x 5 mm in diameter, one plug per fish, 30-50 mg dry weight) samples were obtained from the area beneath the dorsal fin. Fillet samples were obtained from the opposite side of the fish. All samples were freeze-dried and analyzed for total Hg by combustion amalgamation atomic absorption spectrophotometry. Mean relative standard deviations (RSDs) of triplicate samples were similar for all four methods (2.2-2.4%), but the range of RSDs was greater for blood (0.4-5.5%) than for the muscle methods (1.8-4.0%). Total Hg concentrations in muscle were 0.0200-0.8809 ??g/g wet weight; concentrations in plug, needle, and fillet samples from each fish were nearly identical. Blood Hg concentrations were 0.0006-0.0812 ??g/mL and were highly correlated with muscle concentrations; linear regressions between log-transformed blood and fillet Hg concentrations were linear and statistically significant (p methods could provide satisfactory estimates of fillet Hg in smallmouth bass; however, both blood and plug sampling with disposable instruments were easier to perform than needle sampling. The biopsy needle was the most difficult to use, especially on smaller fish, and its relative expense necessitates reuse and, consequently, thorough cleaning between fish to prevent cross-contamination. ?? 2007 Springer Science+Business Media, LLC.

  2. Identification and expression of GnRH2 and GnRH3 in the black sea bass (Centropristis striata), a hermaphroditic teleost.

    Science.gov (United States)

    Morin, Scott J; Decatur, Wayne A; Breton, Timothy S; Marquis, Timothy J; Hayes, Mary K; Berlinsky, David L; Sower, Stacia A

    2015-04-01

    We cloned two cDNAs for two gonadotropin-releasing hormones, GnRH2 (chicken GnRH-II) and GnRH3 (salmon GnRH), respectively, from the black sea bass (Centropristis striata). Black sea bass are protogynous hermaphroditic teleosts that change from females to males between 2 and 5 years of age. Similar to other GnRH precursors, the precursors of black sea bass GnRH2 and GnRH23 consisted of a signal peptide, decapeptide, a downstream processing site, and a GnRH-associated peptide. Our analyses failed to identify GnRH1. GnRH3 precursor transcript was more widely distributed in a variety of tissues compared with GnRH2. Further examination of GnRH expression and gonadal histology was done in black sea bass from three different size groups: small (11.4-44.1 g), medium (179.4-352.2 g) and large (393.8-607.3 g). Interestingly, GnRH3 expression occurred only in the pituitaries of males in the small and medium groups compared with expression of GnRH2. Future functional studies of the sea bass GnRHs will be valuable in elucidating the potential underlying neuroendocrine mechanisms of black sea bass reproduction and may ultimately contribute to management advances in this commercially important fish. PMID:25255937

  3. Assessment of potential impact of the Clinch River Breeder Reactor Plant thermal effluent on the Watts Bar Reservoir striped bass population

    International Nuclear Information System (INIS)

    This report is an assessment of potential adverse impact to striped bass (Morone saxatilis) in Watts Bar Reservoir caused by thermal effluent from operation of the Clinch River Breeder Reactor Plant (CRBRP). The Clinch River arm of Watts Bar Reservoir is occupied by adult striped bass during the warmest months of the year. Concern was raised that operation of the CRBRP, specifically thermal discharges, could conflict with management of striped bass. In all cases examined the thermal plume becomes nearly imperceptible within a short distance from the discharge pipe (about 30 ft [10 m]) compared to river width (about 630 ft [190 m]). Under worst case conditions any presence of the plume in the main channel (opposite side of the river from the discharge) will be confined to the surface layer of the water. An ample portion of river cross sections containing ambient temperature water for passage or residence of adult striped bass will always be available in the vicinity of this thermal effluent. Although a small portion of river cross section would exceed the thermal tolerance of striped bass, the fish would naturally avoid this area and seek out adjacent cooler water. Therefore, it is concluded the CRBRP thermal effluent will not significantly affect the integrity of the striped bass thermal refuge in the Clinch River arm of Watts Bar Reservoir. At this time there is no need to consider alternative diffuser designs and thermal modeling. 8 references, 3 figures, 2 tables

  4. Transcriptional networks associated with the immune system are disrupted by organochlorine pesticides in largemouth bass (Micropterus salmoides) ovary.

    Science.gov (United States)

    Martyniuk, Christopher J; Doperalski, Nicholas J; Feswick, April; Prucha, Melinda S; Kroll, Kevin J; Barber, David S; Denslow, Nancy D

    2016-08-01

    Largemouth bass (Micropterus salmoides) inhabiting Lake Apopka, Florida are exposed to high levels of persistent organochlorine pesticides (OCPs) and dietary uptake is a significant route of exposure for these apex predators. The objectives of this study were to determine the dietary effects of two organochlorine pesticides (p, p'-dichlorodiphenyldichloroethylene; p, p' DDE and methoxychlor; MXC) on the reproductive axis of largemouth bass. Reproductive bass (late vitellogenesis) were fed one of the following diets: control pellets, 125ppm p, p'-DDE, or 10ppm MXC (mg/kg) for 84days. Due to the fact that both p,p' DDE and MXC have anti-androgenic properties, the anti-androgenic pharmaceutical flutamide was fed to a fourth group of largemouth bass (750ppm). Following a 3 month exposure, fish incorporated p,p' DDE and MXC into both muscle and ovary tissue, with the ovary incorporating 3 times more organochlorine pesticides compared to muscle. Endpoints assessed were those related to reproduction due to previous studies demonstrating that these pesticides impact the reproductive axis and we hypothesized that a dietary exposure would result in impaired reproduction. However, oocyte distribution, gonadosomatic index, plasma vitellogenin, and plasma sex steroids (17β-estradiol, E2 and testosterone, T) were not different between control animals and contaminant-fed largemouth bass. Moreover, neither p, p' DDE nor MXC affected E2 or T production in ex vivo oocyte cultures from chemical-fed largemouth bass. However, both pesticides did interfere with the normal upregulation of androgen receptor that is observed in response to human chorionic gonadotropin in ex vivo cultures, an observation that may be related to their anti-androgenic properties. Transcriptomics profiling in the ovary revealed that gene networks related to cell processes such as leukocyte cell adhesion, ossification, platelet function and inhibition, xenobiotic metabolism, fibrinolysis, and thermoregulation

  5. Control of Branchionus sp. and Amoeba sp. in cultures of Arthrospira sp. Control de Branchionus sp. y Amoeba sp. en cultivos de Arthrospira sp.

    Directory of Open Access Journals (Sweden)

    Carlos Méndez

    2012-09-01

    Full Text Available Cultivation of cyanobacterium Arthrospira sp. has been developed in many countries for the production of proteins, pigments and other compounds. Outdoor mass cultures are often affected by biological contamination, drastically reducing productivity as far as bringing death. This study evaluates the control of Branchionus sp. and Amoeba sp. with two chemical compounds: urea (U and ammonium bicarbonate (AB, in laboratory conditions and outdoor mass culture of Arthrospira sp. The lethal concentration 100 (LC100 at 24 h for Branchionus sp. and Amoeba sp. determined was of 60-80 mg L-1 (U and 100-150 mg L-1 (AB. The average effective inhibition concentration for 50% of the population (IC50 in Arthrospira sp., after 72 h, was 80 mg L-1 (U and 150 mg L-1 (AB. The application of doses of 60 mg L-1 (U or 100 mg L-1 (AB in the outdoor mass culture of this contaminated microalga, completely inhibited grazing and did not affect the growth of Arthrospira sp. but rather promoted rapid recovery of algal density at levels prior to infestation. These compounds provided an economical and effective control of predators in cultures of Arthrospira sp.El cultivo de la cianobacteria Arthrospira sp. ha sido desarrollado en muchos países para la obtención de proteínas, pigmentos y otros compuestos. Cultivo que a nivel industrial se ve afectado frecuentemente por contaminación biológica, reduciendo drásticamente la productividad hasta causar la muerte. Este estudio evalúa el control de Branchionus sp. y de Amoeba sp. con dos compuestos químicos, la urea (U y bicarbonato de amonio (AB en cultivos de Arthrospira sp. La concentración letal 100 (LC100 determinada a las 24 h para Branchionus sp. y Amoeba sp. fue de 60-80 mg L-1 (U y 100-150 mg L-1 (AB. La concentración media de inhibición efectiva, después de 72 h, para el 50% de la población (IC50 en Arthrospira fue de 80 mg L-1 (U y 150 mg L-1 (AB. La aplicación de dosis de 60 mg L-1 (U ó 100 mg L-1 (AB en

  6. Dynamics of polychlorinated biphenyls in striped bass from the Hudson river. III. tissue disposition and routes for elimination

    International Nuclear Information System (INIS)

    Striped bass were exposed to 14C-polychlorinated biphenyls (PCB) in single-dose and multiple-dose experiments. Samples were analyzed to determine rate constants for PCB elimination from individual tissues, PCB concentration in tissues, the proportion of the PCB burden retained and the proportion of the cumulative dose retained by each tissue at various times after exposure. An experiment was also conducted to determine both the potential for secondary PCB uptake in dietary exposure studies and the relative tissue disposition of PCBs assimilated from dietary sources as compared to direct water uptake. PCBs were present in the tissues of striped bass within 6 h after administration of a single dose. Certain tissue compartments, such as the liver/gall bladder, accumulated PCBs over a period of 48 h even though the whole-body burden had decreased between 24 and 48 h. Except for the gills, elimination rate constants for all tissues were similar and were to the whole body elimination rate constant. Elimination during the first few hours following exposure to PCBs may be due to equilibrium partitioning from the gill to the environment. The multiple-dose study showed that PCB burdens in striped bass continued to increase with dosing. However, tissue-specific rate constants for PCB elimination led to an increased flux of PCB out of tissues, and an overall decline in the percent of the cumulative dose remaining in the body 48 h after administration of each dose. The most likely route for PCB elimination from striped bass was from tissues to the liver and thence to the intestine via the bile. These were no differences in the tissue disposition of PCB related to route of exposure

  7. San Francisco Estuary Striped Bass Migration History Determined by Electron-microprobe Analysis of Otolith Sr/Ca Ratio

    Energy Technology Data Exchange (ETDEWEB)

    Ostrach, D J; Phillis, C C; Weber, P K; Ingram, B L; Zinkl, J G

    2004-09-17

    Habitat use has been shown to be an important factor in the bioaccumulation of contaminants in striped bass. This study examines migration in striped bass as part of a larger study investigating bioaccumulation and maternal transfer of xenobiotics to progeny in the San Francisco Estuary system. Habitat use, residence time and spawning migration over the life of females (n = 23) was studied. Female striped bass were collected between Knights Landing and Colusa on the Sacramento River during the spawning runs of 1999 and 2001. Otoliths were removed, processed and aged via otolith microstructure. Subsequently, otoliths were analyzed for strontium/calcium (Sr/Ca) ratio using an electron-microprobe to measure salinity exposure and to distinguish freshwater, estuary, and marine habitat use. Salinity exposure during the last year before capture was examined more closely for comparison of habitat use by the maternal parent to contaminant burden transferred to progeny. Results were selectively confirmed by ion microprobe analyses for habitat use. The Sr/Ca data demonstrate a wide range of migratory patterns. Age of initial ocean entry differs among individuals before returning to freshwater, presumably to spawn. Some fish reside in freshwater year-round, while others return to more saline habitats and make periodic migrations to freshwater. Frequency of habitat shifts and residence times differs among fish, as well as over the lifetime of individual fish. While at least one fish spent its final year in freshwater, the majority of spawning fish spent their final year in elevated salinity. However, not all fish migrated to freshwater to spawn in the previous year. Results from this investigation concerning migration history in striped bass can be combined with contaminant and histological developmental analyses to better understand the bioaccumulation of contaminants and the subsequent effects they and habitat use have on fish populations in the San Francisco Estuary system.

  8. Largemouth bass (Micropterus salmoides) and striped mullet (Mugil cephalus) as vectors of contaminants to human consumers in northwest Florida

    Science.gov (United States)

    Karouna-Renier, Natalie K.; Snyder, Richard A.; Lange, Ted; Gibson, Suzanne; Allison, Jeffrey G.; Wagner, Matthew E.; Rao, K. Ranga

    2011-01-01

    The health benefits of regular consumption of fish and seafood have been espoused for many years. However, fish are also a potential source of environmental contaminants that have well known adverse effects on human health. We investigated the consumption risks for largemouth bass (Micropterus salmoides; n = 104) and striped mullet (Mugil cephalus; n = 170), two commonly harvested and consumed fish species inhabiting fresh and estuarine waters in northwest Florida. Skinless fillets were analyzed for total mercury, inorganic arsenic, polychlorinated dibenzo-p-dioxins and polychlorinated dibenzofurans (PCDD/F), polychlorinated biphenyls (PCBs), and organochlorine pesticides. Contaminant levels were compared to screening values (SV) calculated using U.S. Environmental Protection Agency (EPA) recommendations for establishing consumption advisories. Largemouth bass were found to contain high levels of total mercury at all sampling locations (0.37-0.89 ug/g) and one location exhibited elevated total PCBs (39.4 ng/g). All of the samples exceeded Florida fish consumption advisory trigger levels for total mercury and one location exceeded the U.S. EPA SV for total PCBs. As a result of the high mercury levels, the non-cancer health risks (hazard index-HI) for bass were above 1 for all locations. Striped mullet from several locations with known point sources contained elevated levels of PCBs (overall range 3.4-59.3 ng/g). However, total mercury levels in mullet were low. Eight of the 16 mullet sampling locations exceeded the U.S. EPA SV for total PCBs and two locations exceeded an HI of 1 due to elevated PCBs. Despite the elevated levels of total PCBs in some samples, only two locations exceeded the acceptable cancer risk range and therefore cancer health risks from consumption of bass and mullet were determined to be low at most sampling locations.

  9. Growth performance of sea bass fed increasing levels of pea-wheat protein in diets varying in fish meal quality

    Directory of Open Access Journals (Sweden)

    E. Tibaldi

    2010-04-01

    Full Text Available A 11-week trial was carried out to compare the growth performance of sea bass (D. labrax fed six isonitrogenous isocaloric diets where protein from two fish meals of different nutritive value was replaced with graded levels (0, 50 or 75% of a mixture made up by a pea protein concentrate and wheat gluten. Fish meal quality did not affect (P>0.05 weight gain or feed efficiency in fish fed graded levels of plant protein in the diet. Feed intake decreased (P<0.05 as the level of plant protein was increased in the diet but this did not led to impaired growth or feed conversion rate. Protein efficiency and retention were equally improved (P<0.05 only with diets where a poor quality fish meal was substituted by protein rich-plant ingredients. Calculations based on the mass balance of nutrients of sea bass proven the inclusion of a mixture of highly purified plant-protein derivatives in complete diets for the sea bass, to be beneficial in reducing pollution load.

  10. Laser capture microdissection of intestinal tissue from sea bass larvae using an optimized RNA integrity assay and validated reference genes.

    Science.gov (United States)

    Schaeck, M; De Spiegelaere, W; De Craene, J; Van den Broeck, W; De Spiegeleer, B; Burvenich, C; Haesebrouck, F; Decostere, A

    2016-01-01

    The increasing demand for a sustainable larviculture has promoted research regarding environmental parameters, diseases and nutrition, intersecting at the mucosal surface of the gastrointestinal tract of fish larvae. The combination of laser capture microdissection (LCM) and gene expression experiments allows cell specific expression profiling. This study aimed at optimizing an LCM protocol for intestinal tissue of sea bass larvae. Furthermore, a 3'/5' integrity assay was developed for LCM samples of fish tissue, comprising low RNA concentrations. Furthermore, reliable reference genes for performing qPCR in larval sea bass gene expression studies were identified, as data normalization is critical in gene expression experiments using RT-qPCR. We demonstrate that a careful optimization of the LCM procedure allows recovery of high quality mRNA from defined cell populations in complex intestinal tissues. According to the geNorm and Normfinder algorithms, ef1a, rpl13a, rps18 and faua were the most stable genes to be implemented as reference genes for an appropriate normalization of intestinal tissue from sea bass across a range of experimental settings. The methodology developed here, offers a rapid and valuable approach to characterize cells/tissues in the intestinal tissue of fish larvae and their changes following pathogen exposure, nutritional/environmental changes, probiotic supplementation or a combination thereof. PMID:26883391

  11. Victims Themselves of a Close Encounter: On the Sensory Language and Bass Fiction of Space Ape (In Memoriam

    Directory of Open Access Journals (Sweden)

    tobias c. van Veen

    2015-11-01

    Full Text Available This text is written in memoriam to dubstep emcee and poet Space Ape (Stephen Samuel Gordon, b. June 17th, 1970; d. October 2nd, 2014. By his own words, Space Ape arose from the depths of the black Atlantic, on a mission to relieve the “pressure” through bass fiction. My aim is to explicate Space Ape’s bass fiction as the intersection of material and imaginal forces, connecting it to a broader Afrofuturist constellation of mythopoetic becomings. Memory and matter converge in the affect and sounding of Space Ape the “hostile alien” (“Space Ape”, Burial, 2006, a figure shaped at the intersection of the dread body, riddim warfare, and speculative lyricism. Space Ape set out to “xorcise” that which consumed him from within by embracing the “spirit of change”. Turning to process philosophy, I demonstrate how Space Ape’s bass fiction—his virtual body—activates the abstract concepts of becoming in the “close encounter” with the hostile alien.

  12. Consumer preferences regarding the introduction of new organic products. The case of the Mediterranean sea bass (Dicentrarchus labrax) in Italy.

    Science.gov (United States)

    Mauracher, C; Tempesta, T; Vecchiato, D

    2013-04-01

    The introduction of new products on the market poses several challenges; in particular, whether the characteristics of the proposed product will be judged positively by potential consumers. This paper analyses the preferences of consumers regarding the introduction on the Italian market of a new product: organic Mediterranean sea bass. The aim of this study is to assess the importance given by consumers to four main characteristics of sea bass (country of origin, size, production method - organic or conventional - and price) so as to be able to formulate marketing strategies. We applied a choice experiment (CE) in order to define not only the ordinal ranking of preferences but also the willingness to pay (WTP) for the key characteristics of the newly-introduced product. We found that consumers show a higher WTP for the sea bass country of origin than for the breeding method used. Our results suggest that while organic aquaculture might be a new and important strategy for diversification, if suitable communication, either from a public policy or commercial perspective, and labelling/certification are not taken into consideration, the added value of the production method might not be perceived by the final consumers. PMID:23268110

  13. Species profiles: Life histories and environmental requirements of coastal fishes and invertebrates (South Atlantic): Black sea bass

    Energy Technology Data Exchange (ETDEWEB)

    Mercer, L.P.

    1989-07-01

    Species profiles are literature summaries on the taxonomy, morphology, range, life history, and environmental requirements of coastal aquatic species. They are designed to assist in environmental impact assessment. The black sea bass, Centropristis striata, is an abundant species associated with the inshore sponge-coral habitat in the South Atlantic Bight (Cape Hatteras to Cape Canaveral). It is a protogynous hermaphrodite (each individual is first a female and then a male) that spawns from January to June on the Continental shelf. Juveniles utilize estuaries, as well as offshore areas, for nurseries. It is a slow growing species with a life span of about 10 years. Juveniles and adults are bottom-feeding carnivores. Adults have been collected at temperatures as low as 6 /degree/C but are most abundant at temperatures of 8 to 10 /degree/C and above. Juveniles tolerate lower temperatures and greater salinity ranges than adults. Black sea bass are primarily harvested by the recreational hook and line fishery and the commercial trap fishery. Yield-per-recruit analyses indicate that the harvest of black sea bass is less than the maximum possible due to a combination of high fishing pressure and harvest of small fish. 58 refs., 2 figs., 6 tabs.

  14. Safety of oxytetracycline (Terramycin TM-100F) administered in feed to hybrid striped bass, walleyes, and yellow perch

    Science.gov (United States)

    Gaikowski, M.P.; Wolf, J.C.; Schleis, S.M.; Gingerich, W.H.

    2003-01-01

    Oxytetracycline (Terramycin TM-100F, a medicated premix containing oxytetracycline at 220 g/kg) is approved in the United States to control certain systemic bacterial diseases of salmon and catfish when fed at a rate of 55-82.5 mg per kilogram of bodyweight per day for 10 d. Although oxytetracycline may also control certain systemic bacterial infections in coolwater or scaled warmwater fish, no safety data for such species are available. Our objective was to determine the safety of oxytetracycline administered in feed at nominal doses of 0, 82.5, 248, or 413 mg??kg-1??d-1 to yellow perch Perca flavescens and hybrid striped bass (striped bass Morone saxatilis x white bass M. chrysops) for 10 d and to walleye Sander vitreus (formerly Stizostedion vitreum) for 20 d. Yellow perch and hybrid striped bass consumed 50% to 100% of the diet, whereas walleye feed consumption was occasionally less than 50% of the diet. Feed or fecal material was present in the gastrointestinal tract of all necropsied walleyes except for one control fish. The single growth effect was that hybrid striped bass offered a nominal dose of 413 mg??kg-1??d-1 were significantly smaller than untreated controls. Oxytetracycline-related histopathological findings were limited to walleyes and were of low severity. The histopathological findings included decreased hematopoietic-lymphopoietic (H&L) tissue in the anterior kidneys, diffuse hyperplasia of the gill filament epithelium, and a decreased prevalence of fish with eosinophilic droplets in their renal tubular epithelial cells. Although the incidence of decreased H&L tissue tended to increase in proportion to oxytetracycline dose, this finding was statistically significant only for fish that received a nominal dose of 413 mg??kg-1??d-1. Given the pathogenicity of the types of bacteria that are controlled by oxytetracycline treatment and the long history of its use in major aquaculture species, the relative risk of the minor oxytetracycline

  15. SP-100 Test Site

    International Nuclear Information System (INIS)

    Preparatory activities are well under way at Hanford to convert the 309 Containment Building and its associated service wing to a 2.5 MWt nuclear test facility for the SP-100 Ground Engineering System (GES) test. Preliminary design is complete, encompassing facility modifications, a secondary heat transport system, a large vacuum system to enclose the high temperature reactor, a test assembly cell and handling system, control and data processing systems, and safety and auxiliary systems. The design makes extensive use of existing equipment to minimize technical risk and cost. Refurbishment of this equipment is 75% complete. The facility has been cleared of obstructing equipment from its earlier reactor test. Current activities are focusing on definitive design and preparation of the Preliminary Safety Analysis Report (PSAR) aimed at procurement and construction approvals and schedules to achieve reactor criticality by January 1992. 6 refs

  16. Aquatic contaminants alter genes involved in neurotransmitter synthesis and gonadotropin release in largemouth bass

    International Nuclear Information System (INIS)

    Many aquatic contaminants potentially affect the central nervous system, however the underlying mechanisms of how toxicants alter normal brain function are not well understood. The objectives of this study were to compare the effects of emerging and prevalent environmental contaminants on the expression of brain transcripts with a role in neurotransmitter synthesis and reproduction. Adult male largemouth bass (Micropterus salmoides) were injected once for a 96 h duration with control (water or oil) or with one of two doses of a single chemical to achieve the following body burdens (μg/g): atrazine (0.3 and 3.0), toxaphene (10 and 100), cadmium (CdCl2) (0.000067 and 0.00067), polychlorinated biphenyl (PCB) 126 (0.25 and 2.5), and phenanthrene (5 and 50). Partial largemouth bass gene segments were cloned for enzymes involved in neurotransmitter (glutamic acid decarboxylase 65, GAD65; tyrosine hydroxylase) and estrogen (brain aromatase; CYP19b) synthesis for real-time PCR assays. In addition, neuropeptides regulating feeding (neuropeptide Y) and reproduction (chicken GnRH-II, cGnRH-II; salmon GnRH, sGnRH) were also investigated. Of the chemicals tested, only cadmium, PCB 126, and phenanthrene showed any significant effects on the genes tested, while atrazine and toxaphene did not. Cadmium (0.000067 μg/g) significantly increased cGnRH-II mRNA while PCB 126 (0.25 μg/g) decreased GAD65 mRNA. Phenanthrene decreased GAD65 and tyrosine hydroxylase mRNA levels at the highest dose (50 μg/g) but increased cGnRH-II mRNA at the lowest dose (5 μg/g). CYP19b, NPY, and sGnRH mRNA levels were unaffected by any of the treatments. A hierarchical clustering dendrogram grouped PCB 126 and phenanthrene more closely than other chemicals with respect to the genes tested. This study demonstrates that brain transcripts important for neurotransmitter synthesis neuroendocrine function are potential targets for emerging and prevalent aquatic contaminants.

  17. Aquatic contaminants alter genes involved in neurotransmitter synthesis and gonadotropin release in largemouth bass

    Energy Technology Data Exchange (ETDEWEB)

    Martyniuk, Christopher J. [Department of Physiological Sciences and Center for Environmental and Human Toxicology, University of Florida, Gainesville, FL 32611 (United States); Sanchez, Brian C. [Department of Forestry and Natural Resources and School of Civil Engineering, 195 Marsteller St., Purdue University, West Lafayette, IN 47907 (United States); Szabo, Nancy J.; Denslow, Nancy D. [Department of Physiological Sciences and Center for Environmental and Human Toxicology, University of Florida, Gainesville, FL 32611 (United States); Sepulveda, Maria S., E-mail: mssepulv@purdue.edu [Department of Forestry and Natural Resources and School of Civil Engineering, 195 Marsteller St., Purdue University, West Lafayette, IN 47907 (United States)

    2009-10-19

    Many aquatic contaminants potentially affect the central nervous system, however the underlying mechanisms of how toxicants alter normal brain function are not well understood. The objectives of this study were to compare the effects of emerging and prevalent environmental contaminants on the expression of brain transcripts with a role in neurotransmitter synthesis and reproduction. Adult male largemouth bass (Micropterus salmoides) were injected once for a 96 h duration with control (water or oil) or with one of two doses of a single chemical to achieve the following body burdens ({mu}g/g): atrazine (0.3 and 3.0), toxaphene (10 and 100), cadmium (CdCl{sub 2}) (0.000067 and 0.00067), polychlorinated biphenyl (PCB) 126 (0.25 and 2.5), and phenanthrene (5 and 50). Partial largemouth bass gene segments were cloned for enzymes involved in neurotransmitter (glutamic acid decarboxylase 65, GAD65; tyrosine hydroxylase) and estrogen (brain aromatase; CYP19b) synthesis for real-time PCR assays. In addition, neuropeptides regulating feeding (neuropeptide Y) and reproduction (chicken GnRH-II, cGnRH-II; salmon GnRH, sGnRH) were also investigated. Of the chemicals tested, only cadmium, PCB 126, and phenanthrene showed any significant effects on the genes tested, while atrazine and toxaphene did not. Cadmium (0.000067 {mu}g/g) significantly increased cGnRH-II mRNA while PCB 126 (0.25 {mu}g/g) decreased GAD65 mRNA. Phenanthrene decreased GAD65 and tyrosine hydroxylase mRNA levels at the highest dose (50 {mu}g/g) but increased cGnRH-II mRNA at the lowest dose (5 {mu}g/g). CYP19b, NPY, and sGnRH mRNA levels were unaffected by any of the treatments. A hierarchical clustering dendrogram grouped PCB 126 and phenanthrene more closely than other chemicals with respect to the genes tested. This study demonstrates that brain transcripts important for neurotransmitter synthesis neuroendocrine function are potential targets for emerging and prevalent aquatic contaminants.

  18. Aquatic contaminants alter genes involved in neurotransmitter synthesis and gonadotropin release in largemouth bass.

    Science.gov (United States)

    Martyniuk, Christopher J; Sanchez, Brian C; Szabo, Nancy J; Denslow, Nancy D; Sepúlveda, Maria S

    2009-10-19

    Many aquatic contaminants potentially affect the central nervous system, however the underlying mechanisms of how toxicants alter normal brain function are not well understood. The objectives of this study were to compare the effects of emerging and prevalent environmental contaminants on the expression of brain transcripts with a role in neurotransmitter synthesis and reproduction. Adult male largemouth bass (Micropterus salmoides) were injected once for a 96 h duration with control (water or oil) or with one of two doses of a single chemical to achieve the following body burdens (microg/g): atrazine (0.3 and 3.0), toxaphene (10 and 100), cadmium (CdCl(2)) (0.000067 and 0.00067), polychlorinated biphenyl (PCB) 126 (0.25 and 2.5), and phenanthrene (5 and 50). Partial largemouth bass gene segments were cloned for enzymes involved in neurotransmitter (glutamic acid decarboxylase 65, GAD65; tyrosine hydroxylase) and estrogen (brain aromatase; CYP19b) synthesis for real-time PCR assays. In addition, neuropeptides regulating feeding (neuropeptide Y) and reproduction (chicken GnRH-II, cGnRH-II; salmon GnRH, sGnRH) were also investigated. Of the chemicals tested, only cadmium, PCB 126, and phenanthrene showed any significant effects on the genes tested, while atrazine and toxaphene did not. Cadmium (0.000067 microg/g) significantly increased cGnRH-II mRNA while PCB 126 (0.25 microg/g) decreased GAD65 mRNA. Phenanthrene decreased GAD65 and tyrosine hydroxylase mRNA levels at the highest dose (50 microg/g) but increased cGnRH-II mRNA at the lowest dose (5 microg/g). CYP19b, NPY, and sGnRH mRNA levels were unaffected by any of the treatments. A hierarchical clustering dendrogram grouped PCB 126 and phenanthrene more closely than other chemicals with respect to the genes tested. This study demonstrates that brain transcripts important for neurotransmitter synthesis neuroendocrine function are potential targets for emerging and prevalent aquatic contaminants. PMID:19781795

  19. Use of stable carbon and nitrogen isotopes to trace the larval striped bass food chain in the Sacramento-San Joaquin Estuary, California, April to September 1985

    Science.gov (United States)

    Rast, Walter; Sutton, J.E.

    1989-01-01

    To assess one potential cause for the decline of the striped bass fishery in the Sacramento-San Joaquin Estuary, stable carbon and nitrogen isotope ratios were used to examine the trophic structures of the larval striped bass food chain, and to trace the flux of these elements through the food chain components. Study results generally confirm a food chain consisting of the elements, phytoplankton/detritus-->zooplankton/Neomysis shrimp-->larval striped bass. The stable isotope ratios generally become more positive as one progresses from the lower to the higher trophic level food chain components, and no unusual trophic structure was found in the food chain. However, the data indicate an unidentified consumer organism occupying an intermediate position between the lower and higher trophic levels of the larval striped bass food chain. Based on expected trophic interactions, this unidentified consumer would have a stable carbon isotope ratio of about 28/mil and a stable nitrogen isotope ratio of about 8/mi. Three possible feeding stages for larval striped bass also were identified, based on their lengths. The smallest length fish seem to subsist on their yolk sac remnants, and the largest length fish subsist on Neomysis shrimp and zooplankton. The intermediate-length fish represent a transition stage between primary food sources and/or use of a mixture of food sources. (USGS)

  20. Forecasting sales of new vehicle with limited data using Bass diffusion model and Grey theory

    Science.gov (United States)

    Abu, Noratikah; Ismail, Zuhaimy

    2015-02-01

    New product forecasting is a process that determines a reasonable estimate of sales attainable under a given set of conditions. There are several new products forecasting method in practices and Bass Diffusion Model (BDM) is one of the most common new product diffusion model used in many industries to forecast new product and technology. Hence, this paper proposed a combining BDM with Grey theory to forecast sales of new vehicle in Malaysia that certainly have limited data to build a model on. The aims of this paper is to examine the accuracy of different new product forecasting models and thus identify which is the best among the basic BDM and combining BDM with Grey theory. The results show that combining BDM with Grey theory performs better than the basic BDM based on in-sample and out-sample mean absolute percentage error (MAPE). Results also reveals combining model forecast more effectively and accurately even with insufficient previous data on the new vehicle in Malaysia.

  1. Intestinal alterations in European sea bass Dicentrarchus labrax (Linnaeus, 1758) exposed to microplastics: Preliminary results.

    Science.gov (United States)

    Pedà, Cristina; Caccamo, Letteria; Fossi, Maria Cristina; Gai, Francesco; Andaloro, Franco; Genovese, Lucrezia; Perdichizzi, Anna; Romeo, Teresa; Maricchiolo, Giulia

    2016-05-01

    This study investigates, for the first time, the intestinal responses of European sea bass Dicentrarchus labrax chronically exposed to microplastics through ingestion. Fish (n = 162) were fed with 3 different treatment diets for 90 days: control, native polyvinyl chloride (PVC) and polluted polyvinyl chloride (PVC) pellets. Intestines were fixed and processed for histological analysis using standard techniques. Histopathological alterations were examined using a score value (from 0 to 4). The distal part of intestine in all samples proved to be the most affected by pathological alterations, showing a gradual change varying from moderate to severe related to exposure times. The histological picture that characterizes both groups especially after 90 days of exposure, suggests that the intestinal functions can be in some cases totally compromised. The worst condition is increasingly evident in the distal intestine of fish fed with polluted PVC pellets respect to control groups (p < 0.05) to different exposure times. These first results underline the need to assess the impact of increasing microplastics pollution on the marine trophic web. PMID:26851981

  2. Multivariate approach to gill pathology in European sea bass after experimental exposure to cadmium and terbuthylazine.

    Science.gov (United States)

    Manera, Maurizio; Sayyaf Dezfuli, Bahram; DePasquale, Joseph A; Giari, Luisa

    2016-07-01

    The combined use of guided quantitative expert analysis and of multivariate exploratory data analysis is reported as a robust, sensitive and sufficiently specific approach to study European sea bass gill secondary lamellar pathology after exposure to incremental doses of cadmium and terbuthylazine up to 48h. The following elementary pathological findings were considered: "epithelial lifting", "epithelial shrinkage", "epithelial swelling", "pillar cells coarctation", "pillar cells detachment", "channels fusion", "chloride cells swelling", and "chloride cells invasion". The relative spatial extension was determined according to exposure class and data were analyzed by means of canonical correspondence analysis (CCA), linear discriminant analysis (LDA) and canonical variates analysis (CVA). Histologically and ultrastructurally, cellular shrinkage/coarctation prevailed in cadmium exposed lamellae, whereas cellular swelling and epithelial lifting were predominant in terbuthylazine exposed lamellae compared to unexposed fish. Both CCA and CVA permit a good graphical data grouping according to exposure classes by means of the convex hull minimum polygons. This also reveals exposure dose and time gradients in CCA plot. Accordingly, epithelial swelling and epithelial shrinkage were comparatively associated to higher exposure time, whereas epithelial shrinkage and pillar cells coarctation were comparatively associated to higher exposure dose. LDA with only "epithelial shrinkage", "epithelial swelling" and "pillar cells coarctation" in the model classified correctly 87.5% of the cross-validated cases. A possible pathogenetic relationship between the discriminant elementary lesions and the toxic mode of action at the cellular level of both cadmium and terbuthylazine is also discussed. PMID:27057996

  3. The association of DNA damage to concentrations of mercury and radiocesium in largemouth bass

    International Nuclear Information System (INIS)

    Largemouth bass from five lakes were examined to determine levels of contamination by mercury and radiocesium and amounts of DNA damage. Concentrations of these toxicants and an index of body condition were regressed against overall DNA damage and DNA damage in individual tissues (liver, gills, and red blood cells) as indicated by the alkaline unwinding method. Sample sites showed considerable heterogeneity in concentrations of mercury and radiocesium, as well as numbers of DNA strand breaks. Generally, increased concentrations of toxicants were related to increased DNA damage. Tissues may have responded to contaminants in different manners; red blood cells generally showed the greatest DNA damage while liver tissue showed the least. Although body condition was related to DNA damage, it is unclear whether it has a direct effect or whether it is a correlated response to contamination by mercury and radiocesium. The potential for repair of DNA strand breaks and cell turnover rates may play an important role in determining the ultimate amount of DNA damage in contaminated organisms

  4. Chemical residues and biochemical responses in wild and cultured European sea bass (Dicentrarchus labrax L.)

    International Nuclear Information System (INIS)

    Cultured and wild sea bass (Dicentrarchus labrax) from the Arade Estuary were sampled in summer and winter and the degree of exposure to metals and polycyclic aromatic hydrocarbons (PAHs) assessed, together with some biochemical responses against those and other pollutants. The highest levels of copper (up to 997 μg g-1 dry weight) and cadmium (up to 4.22 μg g-1 dry weight) were detected in the liver and kidney of cultured specimens, whereas the highest exposure to PAHs was observed in wild fish. Significant alterations in some biochemical markers were detected and associated to pollutant exposure. Thus, metallothionein concentrations were higher in the tissues of cultured fish and positively correlated with metal residues. The activity 7-ethoxyresorufin O-deethylase ranged from 28 pmol/min/mg protein in cultured fish to 83 pmol/min/mg protein in wild fish collected near a marina area. Cultured fish and wild fish from the marina area had depressed acetylcholinesterase in muscle tissue and a parasitic infection in the gonads. The obtained results support the usefulness of the combined use of chemical and biochemical markers to assess the impact of anthropogenic pollutants in both wild and cultured fish

  5. Skeletal deformities in smallmouth bass, Micropterus dolomieui, from southern Appalachian reservoirs

    Energy Technology Data Exchange (ETDEWEB)

    Van den Avyle, M.J.; Garvick, S.J.; Blazer, V.S.; Hamilton, S.J.; Brumbaugh, W.G.

    1989-09-01

    Smallmouth bass (Micropterus dolomieui) populations in two of five reservoirs sampled in the southern Appalachian Mountains contained high percentages of individuals with lordosis, kyphosis, or scoliosis. Deformities of the vertebral column occurred in several year classes and varied with fish size; they were absent in small fish, present in 25-30% of the fish 241-300 mm long, and then decreased in occurrence with increased length. Because environmental contamination is often responsible for high occurrences of deformed fish, whole-body concentrations of contaminants, bone development characteristics, and blood plasma concentrations of calcium and phosphorus in normal and deformed fish were measured and compared the results with those for fish from reservoirs where no deformities were found. Vertebrae were significantly weaker and more elastic in deformed than in normal fish, but biochemical properties of vertebrae were similar among the groups tested. Concentrations of pesticides and metals were not elevated in deformed fish, and concentrations of calcium and phosphorus in blood plasma were similar in normal and deformed groups. Most environmental contaminants that have been shown to cause fish deformities could be discounted as causative agents on the basis of these results; however, the exact cause was not determined. Further attempts to diagnose the cause of the deformities were limited by the lack of background information on relationships among bone development processes, types of stresses that cause deformities, and types of bone tissue in fish.

  6. Structure and Specificity of a Binary Tandem Domain F-Lectin from Striped Bass (Morone saxatilis)

    Energy Technology Data Exchange (ETDEWEB)

    Bianchet, M.; Odom, E; Vasta, J; Amzel, M

    2010-01-01

    The plasma of the striped bass Morone saxatilis contains a fucose-specific lectin (MsaFBP32) that consists of two F-type carbohydrate recognition domains (CRDs) in tandem. The crystal structure of the complex of MsaFBP32 with l-fucose reported here shows a cylindrical 81-A-long and 60-A-wide trimer divided into two globular halves: one containing N-terminal CRDs (N-CRDs) and the other containing C-terminal CRDs (C-CRDs). The resulting binding surfaces at the opposite ends of the cylindrical trimer have the potential to cross-link cell surface or humoral carbohydrate ligands. The N-CRDs and C-CRDs of MsaFBP32 exhibit significant structural differences, suggesting that they recognize different glycans. Analysis of the carbohydrate binding sites provides the structural basis for the observed specificity of MsaFBP32 for simple carbohydrates and suggests that the N-CRD recognizes more complex fucosylated oligosaccharides and with a relatively higher avidity than the C-CRD. Modeling of MsaFBP32 complexed with fucosylated glycans that are widely distributed in prokaryotes and eukaryotes rationalizes the observation that binary tandem CRD F-type lectins function as opsonins by cross-linking 'non-self' carbohydrate ligands and 'self' carbohydrate ligands, such as sugar structures displayed by microbial pathogens and glycans on the surface of phagocytic cells from the host.

  7. Biomarker responses in sunfish species and largemouth bass from the Saluda River, South Carolina.

    Science.gov (United States)

    Mierzejewski, Jessica; Haney, Dennis C; van den Hurk, Peter

    2014-12-01

    The upstate and Piedmont region of South Carolina is a rapidly urbanizing area as a result of a steadily growing population. This increase in population and development has the potential to negatively impact local aquatic systems like the Saluda River due to increased pollution from runoff, and effluents from industrial and wastewater treatment facilities. During the summer months of 2010, 159 fish from the Centrarchidae family (sunfish species (Lepomis) and largemouth bass - Micropterus salmoides) were collected from 13 sites along the Saluda River. A suite of biomarker assays, including ethoxyresosufin-O-deethylase, bile fluorescence, glutathione S-transferase, thiobarbituric acid reactive substances, bile estrogens, acetylcholinesterase inhibition, metallothionein and tissue metal levels were applied to investigate the impacts of diminished water quality on fish health. Results indicate that fish from the Saluda River are responding to contamination in a site specific manner, with up to four significant biomarker responses in the most impacted sites. Sampling sites in the lower portion of the Saluda watershed are less impacted by pollution than the upper and central sections. The observed biomarker responses can be explained by the proximity of urban areas, point sources and general land use, and demonstrate the applicability of biomarkers in environmental biomonitoring programs. PMID:25173848

  8. Gill Na+, K+-ATPase activity in largemouth bass (Micropterus salmoides) inhabiting reservoirs contaminated with mercury

    International Nuclear Information System (INIS)

    Active transport of Na+ and K+ for osmoregulation in fish involves gill Na+, K+-ATPase, a membrane-bound enzyme powered by hydrolysis of ATP. Na+, K+-ATPase is inhibited by many dissolved metals including Al, Cd, Cu and Hg, resulting in ionoregulatory dysfunction. However, dissolved Hg concentrations are quite low in most aquatic systems, and dietary sources are the most important contributors to Hg burdens in fish. One recent study demonstrated relationships between muscle Hg concentration and gill Na+, K+-ATPase in a marine fish, suggesting that Hg accumulated via diet can affect osmoregulation. The authors tested for such a relationship in several age-classes of a freshwater fish (Micropterus salmoides) collected from three reservoirs. Fish from Par Pond and L Lake, on the USDOE Savannah River Site in South Carolina had relatively high Hg content: for Par Pond, muscle and liver ranged from 1.58--12.01 and 1.46--23.22 microg Hg/g dry mass, respectively, and for L Lake muscle and liver ranged from 3.11--5.16 and 1.28--12.59 microg Hg/g dry mass, respectively. Bass from an offsite location, Thurmond Lake, had significantly (P +, K+-ATPase activity was not evident

  9. Mycobiota and Aflatoxin B1 in Feed for Farmed Sea Bass (Dicentrarchus labrax

    Directory of Open Access Journals (Sweden)

    Fernando Manuel d´Almeida Bernardo

    2011-02-01

    Full Text Available The safety characteristics of feed used in fish and crustacean aquaculture systems are an essential tool to assure the productivity of those animal exploitations. Safety of feed may be affected by different hazards, including biological and chemical groups. The aim of this preliminary study was to evaluate fungi contamination and the presence of aflatoxins in 87 samples of feed for sea bass, collected in Portugal. Molds were found in 35 samples (40.2% in levels ranging from 1 to 3.3 log10 CFU∙g−1. Six genera of molds were found. Aspergillus flavus was the most frequent, found in all positive samples, with a range from 2 to 3.2 log10 CFU∙g−1. Aspergillus niger was found in 34 samples (39.1%, ranging from 1 to 2.7 log10 CFU∙g−1. Aspergillus glaucus was found in 26 samples (29.9% with levels between 1 and 2.4 log10 CFU∙g−1. Penicillium spp. and Cladosporium spp. were both found in 25 samples (28.7%. Fusarium spp. was found in 22 samples (25.3%, ranging from 1 to 2.3 log10 CFU∙g−1. All feed samples were screened for aflatoxins using a HPLC technique, with a detection limit of 1.0 μg∙kg−1. All samples were aflatoxin negative.

  10. Bradysia sp. em morangueiro Bradysia sp. in strawberry

    Directory of Open Access Journals (Sweden)

    Bernadete Radin

    2009-04-01

    Full Text Available No trabalho, relatam-se os primeiros registros de Bradysia sp. (Insecta: Diptera: Sciaridae em morangueiro (Fragaria x ananassa Duch., cultivado no Município de Eldorado do Sul, RS. O cultivo foi realizado em sacolas com três metros de comprimento, preenchidas com substrato composto de casca de arroz e turfa, dispostas horizontalmente sobre bancadas de madeira, em ambiente protegido. A presença de Bradysia sp. foi observada na segunda quinzena de agosto de 2005. Neste trabalho, estão descritos os sintomas apresentados no morangueiro pela praga, prováveis conseqüências sobre o aparecimento de doenças e uma breve descrição morfológica da Bradysia sp., adulto e fase larval.This paper describes the first record of Bradysia sp. (Insecta; Diptera; Sciaridae in strawberry (Fragaria x ananassa, cultivated in the city of Eldorado do Sul, RS, Brazil. Strawberry was planted in plastic bags filled with a mixture of burnt rice hulls and peat and cultivated in a greenhouse. The presence of Bradysia sp was noticed in the second fortnight of August, 2005. The symptoms in strawberry and the probable consequences in terms of disease arising were described in the present study, as well as the morphological characterization of Bradysia sp. and its illustrations.

  11. Fertilization strategies for Sea Bass Dicentrarchus labrax (Linnaeus, 1758): effects of pre-incubation and duration of egg receptivity in seawater

    DEFF Research Database (Denmark)

    Siddique, Mohammad A Momin; Butts, Ian; Linhart, Otomar;

    2015-01-01

    for both these treatments after 3 min of pre-incubation, which clearly indicates that sea bass eggs are able to be fertilized by sperm for up to 3 min after release into seawater. This study has particular importance for understanding fertilization strategies, reproductive potential, as well as......Studying gamete biology can provide important information about a species fertilization strategy as well as their reproductive ecology. Currently, there is a lack of knowledge about how long sea bass Dicentrarchus labrax eggs can remain viable after being activated in seawater. The objectives of...... this study were to understand the effects of pre-incubation of fresh and overripe sea bass eggs in seawater and to determine the duration of egg receptivity. Pooled eggs (fresh and overripe) from four females were pre-incubated in seawater for 0 min (control), 0.5 min, 1 min, 3 min, 10 min and 30 min...

  12. SP-100 advanced technology program

    International Nuclear Information System (INIS)

    The goal of the triagency SP-100 Program is to develop long-lived, compact, lightweight, survivable nuclear reactor space power systems for application to the power range 50 kWe to 1 MWe. The successful development of these systems should enable or significantly enhance many of the future NASA civil and commercial missions. The NASA SP-100 Advanced Technology Program strongly augments the parallel SP-100 Ground Engineering System Development program and enhances the chances for success of the overall SP-100 program. The purpose of this paper is to discuss the key technical elements of the Advanced Technology Program and the progress made in the initial year and a half of the project

  13. [Cloning, physical and chemical property analysis of the Japanese sea bass Wap65-2 gene and its expression following Vibrio harveyi infection].

    Science.gov (United States)

    Shi, Yu-Hong; Chen, Jiong; Gao, Shan-Shan; Shen, Guang-Qiang; Lu, Xin-Jiang; Li, Ming-Yun

    2012-10-01

    The warm temperature acclimation related 65 kDa protein-2 (Wap65-2), a teleost plasma glycoprotein, plays an important role in immune regulation against bacterial infection. Here, for the first time we determined the full length cDNA sequence of the Japanese sea bass Wap65-2 gene (1 601 bp in length excluding the 3'-polyA tail). The sequence contains an open reading frame that encodes a protein of 436 amino acids with a molecular weight of 4.87×10(4). The predicted protein had a signal peptide in the N-terminal domain containing 19 residues. Sequence comparison and phylogenetic tree analysis showed that the Japanese sea bass Wap65-2 has a relatively high similarity to the Dicentrarchus labrax Wap65-2. In the healthy Japanese sea bass, Wap65-2 mRNA was expressed mainly in the liver and weakly in the heart and muscle. qRT-PCR results revealed that liver Wap65-2 transcripts were significantly increased after a Vibrio harveyi infection, and peaked 24 hour post injection (6.89 fold increase). The Japanese sea bass Wap65-2 protein was expressed in Escherichia coli and subsequently used for antiserum preparation. Western blot analysis showed that Wap65-2 was significantly increased in V. harveyi infected Japanese sea bass and reached a maximum of 5.33-fold increase at 36 h. In conclusion, the alteration of Japanese sea bass Wap65-2 expression was tightly associated with the progression of the V. harveyi bacterial infection. PMID:23019029

  14. Improving oral hygiene skills by computer-based training: a randomized controlled comparison of the modified Bass and the Fones techniques.

    Directory of Open Access Journals (Sweden)

    Daniela Harnacke

    Full Text Available BACKGROUND: Gingivitis and other plaque-associated diseases have a high prevalence in western communities even though the majority of adults report daily oral hygiene. This indicates a lack of oral hygiene skills. Currently, there is no clear evidence as to which brushing technique would bring about the best oral hygiene skills. While the modified Bass technique is often recommended by dentists and in textbooks, the Fones technique is often recommended in patient brochures. Still, standardized comparisons of the effectiveness of teaching these techniques are lacking. METHODOLOGY/PRINCIPAL FINDINGS: In a final sample of n = 56 students, this multidisciplinary, randomized, examiner-blinded, controlled study compared the effects of parallel and standardized interactive computer presentations teaching either the Fones or the modified Bass technique. A control group was taught the basics of tooth brushing alone. Oral hygiene skills (remaining plaque after thorough oral hygiene and gingivitis were assessed at baseline and 6, 12, and 28 weeks after the intervention. We found a significant group×time interaction for gingivitis (F(4/102 = 3.267; p = 0.016; ε = 0.957; η(2 = 0.114 and a significant main effect of group for oral hygiene skills (F(2/51 = 7.088; p = 0.002; η(2 = 0.218. Fones was superior to Bass; Bass did not differ from the control group. Group differences were most prominent after 6 and 12 weeks. CONCLUSIONS/SIGNIFICANCE: The present trial indicates an advantage of teaching the Fones as compared to the modified Bass technique with respect to oral hygiene skills and gingivitis. Future studies are needed to analyze whether the disadvantage of teaching the Bass technique observed here is restricted to the teaching method employed. TRIAL REGISTRATION: German Clinical Trials Register DRKS00003488.

  15. Organic Nutrition of Beggiatoa sp

    OpenAIRE

    1981-01-01

    Culture OH-75-B of Beggiatoa sp. differed significantly from any described previously in its utilization of organic carbon and reduced sulfur compounds. It deposited internal sulfur granules characteristic of Beggiatoa sp. with either sulfide or thiosulfate in the medium. This strain (OH-75-B, clone 2a) could be grown in agitated liquid cultures on mineral medium with acetate as the only source of organic carbon. The resultant growth yields and rates were comparable to those for typical heter...

  16. Optimal spænding i kajakken

    DEFF Research Database (Denmark)

    Rossing, Niels Nygaard

    2012-01-01

    Artiklens overordnede fokus er, hvilken betydning den enkelte kajakroers spændingsniveau har for præstationen. Artiklen svarer på, hvad et spændingsniveau er, hvilke kilder der er til spænding, og sidst hvad man kan gøre for at finde sit optimale spændingsniveau....

  17. En moderne spøgelseshistorie

    DEFF Research Database (Denmark)

    Simonsen, Karen-Margrethe

    2011-01-01

    Anmeldelse af César Airas "Spøgelserne". Anmeldelsen indkredser bla. spøgelsernes karakter i denne moderne, interkulturelle historie......Anmeldelse af César Airas "Spøgelserne". Anmeldelsen indkredser bla. spøgelsernes karakter i denne moderne, interkulturelle historie...

  18. Fungi Association with Cedar’s Seeds and Fusarium sp. and Pestalotia sp. Pathogenicities Levantamento de Fungos em Sementes de Cedro e Avaliação da Patogenicidade de Fusarium sp. e Pestalotia sp.

    OpenAIRE

    Sílvia Cristina Benetti; Álvaro Figueredo dos Santos; Antonio Carlos de Souza Medeiros; David de Souza Jaccoud Filho

    2010-01-01

    This research had as main objectives the evaluation of cedar’s seeds seed-borne fungi and the Fusarium
    sp. and Pestalotia sp. pathogenicities. For the fungi detection Potato Dextrose and Agar medium and Blotter
    test methods were used. For the pathogenicity tests, isolates of Fusarium sp. and Pestalotia sp. were used. The following fungi were observed: Pestalotia sp., Fusarium sp., Phomopsis sp., Colletotrichum sp., Macrophomina sp. and Cladosporium sp. The fungi...

  19. Reproductive endocrine disruption in smallmouth bass (Micropterus dolomieu) in the Potomac River basin: spatial and temporal comparisons of biological effects

    Science.gov (United States)

    Blazer, Vicki; Iwanowicz, Luke R.; Henderson, Holly; Mazik, Patricia M.; Jenkins, Jill A.; Alvarez, David A.; Young, John A.

    2012-01-01

    A high prevalence of intersex or testicular oocytes (TO) in male smallmouth bass within the Potomac River drainage has raised concerns as to the health of the river. Studies were conducted to document biomarker responses both temporally and spatially to better understand the influence of normal physiological cycles, as well as water quality and land-use influences. Smallmouth bass were collected over a 2-year period from three tributaries of the Potomac River: the Shenandoah River, the South Branch Potomac and Conococheague Creek, and an out-of-basin reference site on the Gauley River. The prevalence of TO varied seasonally with the lowest prevalence observed in July, post-spawn. Reproductive maturity and/or lack of spawning the previous spring, as well as land-use practices such as application of manure and pesticides, may influence the seasonal observations. Annual, seasonal, and site differences were also observed in the percentage of males with measurable concentrations of plasma vitellogenin, mean concentration of plasma vitellogenin in females, and plasma concentrations of 17β-estradiol and testosterone in both sexes. Bass collected in the South Branch Potomac (moderate to high prevalence of TO) had less sperm per testes mass with a lower percentage of those sperm being motile when compared to those from the Gauley River (low prevalence of TO). An inverse relationship was noted between TO severity and sperm motility. An association between TO severity and wastewater treatment plant flow, percent of agriculture, total number of animal feeding operations, the number of poultry houses, and animal density within the catchment was observed.

  20. Preliminary investigation by liquid chromatography tandem mass spectrometry of perfluorinated compounds presence in bass reared and fished in Italy

    Directory of Open Access Journals (Sweden)

    Federica Farabegoli

    2013-12-01

    Full Text Available The term perfluorinated compounds (PFCs defines synthetic fully-fluorinated compounds; since the 50s they have been largely employed in a wide range of industrial and consumer applications, due to their chemical and physical properties. Only recently, research on their potential adverse effects on both the environment and human health has been conducted, highlighting carcinogenic activity, toxic effects on reproduction, liver and kidney. Human exposure to PFCs is mainly through diet, and in particular fishery products are one of the most contaminated food. Perfluorooctane sulfonate (PFOS and perfluorooctanoic acid (PFOA are the two most important and investigated compounds of this group. The aim of this work was to carry out a preliminary monitoring on the presence of these two molecules in 50 bass muscle samples, of which 30 reared and 20 fished, collected from different Italian areas of the Mediterranean Sea. The method used was based on extraction with organic solvent followed by two purification steps: one with salts and one with dispersive solid phase. The extract was finally analysed by means of an ultra performance liquid chromatographytandem mass spectrometric system. Data showed a relevant contamination of this species and evidenced a significant difference of concentrations between fished (PFOS 112.4- >2000 ng/L and PFOA 3.3-487.0 ng/L and reared basses (PFOS 11.1-104.5 ng/L and PFOA <3-51.4 ng/L. Even if these levels of contamination are sensibly lower than those observed in several studies, fished basses could represent a more relevant source of PFCs exposure in human diet.

  1. Sensitive periods for 17β-estradiol exposure during immune system development in sea bass head kidney.

    Science.gov (United States)

    Seemann, Frauke; Knigge, Thomas; Duflot, Aurélie; Marie, Sabine; Olivier, Stéphanie; Minier, Christophe; Monsinjon, Tiphaine

    2016-06-01

    An increasing body of evidence suggests that sex steroids play an important role in the development and regulation of vertebrate immune defense. Therefore, compounds with estrogenic activity may influence the immune system via receptor-mediated pathways. The presence of estrogen receptors in immune cells and organs during the early stages of development may indicate that female steroid hormones are involved in the maturation of the fish immune system. This is of particular importance, as some marine fish are probably exposed to sources of exogenous estrogens while they reside in their estuarine nursery grounds. In this study, the influence of 17β-estradiol (E2) on estrogen receptor and cytokine gene expression was assessed in juvenile sea bass (Dicentrarchus labrax) together with characterization of the head kidney leukocyte populations and corresponding phagocytic activity during organ regionalization from 98 to 239 dph. E2 exposure, beginning at 90 dph resulted in indirect and delayed modifications of interleukin 1β and estrogen receptor α gene expression, which may affect B-lymphocyte proliferation in the sea bass head kidney. The E2 treatment of 120 dph fish led to an increase in estrogen receptor β2 and a decrease in transforming growth factor β1 gene expression, which coincided with decreased phagocytic activity of head kidney lymphocytes and monocytes/macrophages. Additionally, these changes were observed during developmental periods described as critical phases for B-lymphocyte development in mammals. Consequently, exogenous estrogens have the potential to modify the innate immune response in juvenile sea bass and to exert detrimental effects on head kidney development. Copyright © 2015 John Wiley & Sons, Ltd. PMID:26278277

  2. 3-D linear inversion of gravity data: method and application to Basse-Terre volcanic island, Guadeloupe, Lesser Antilles

    Science.gov (United States)

    Barnoud, Anne; Coutant, Olivier; Bouligand, Claire; Gunawan, Hendra; Deroussi, Sébastien

    2016-04-01

    We use a Bayesian formalism combined with a grid node discretization for the linear inversion of gravimetric data in terms of 3-D density distribution. The forward modelling and the inversion method are derived from seismological inversion techniques in order to facilitate joint inversion or interpretation of density and seismic velocity models. The Bayesian formulation introduces covariance matrices on model parameters to regularize the ill-posed problem and reduce the non-uniqueness of the solution. This formalism favours smooth solutions and allows us to specify a spatial correlation length and to perform inversions at multiple scales. We also extract resolution parameters from the resolution matrix to discuss how well our density models are resolved. This method is applied to the inversion of data from the volcanic island of Basse-Terre in Guadeloupe, Lesser Antilles. A series of synthetic tests are performed to investigate advantages and limitations of the methodology in this context. This study results in the first 3-D density models of the island of Basse-Terre for which we identify: (i) a southward decrease of densities parallel to the migration of volcanic activity within the island, (ii) three dense anomalies beneath Petite Plaine Valley, Beaugendre Valley and the Grande-Découverte-Carmichaël-Soufrière Complex that may reflect the trace of former major volcanic feeding systems, (iii) shallow low-density anomalies in the southern part of Basse-Terre, especially around La Soufrière active volcano, Piton de Bouillante edifice and along the western coast, reflecting the presence of hydrothermal systems and fractured and altered rocks.

  3. Tissue distribution of organochlorine pesticides in largemouth bass (Micropterus salmoides) from laboratory exposure and a contaminated lake.

    Science.gov (United States)

    Dang, Viet D; Kroll, Kevin J; Supowit, Samuel D; Halden, Rolf U; Denslow, Nancy D

    2016-09-01

    Tissue concentrations of persistent organochlorine pesticides in laboratory-exposed largemouth bass (Micropterus salmoides) and in bass collected from Lake Apopka, FL were determined by both total mass and lipid normalized mass to better understand the bioaccumulation pathways of contaminants. In the laboratory study, male bass were orally administered a single dose of a mixture of two pesticides (p,p'-dichlorodiphenyldichloroethylene (p,p'-DDE) and dieldrin) and then fed uncontaminated food for 28 days. Gastrointestinal tract, liver, brain, gonad, kidney, spleen, and muscle were collected for chemical analysis. Different profiles were observed by total contaminant mass in tissues compared to lipid normalized mass. On a lipid normalized basis, p,p'-DDE was highest in the gastrointestinal tract followed by the liver, gonad, spleen, muscle, kidney and then brain. Dieldrin, on the other hand, was highest in the gastrointestinal tract and spleen and then followed by the gonad, muscle, liver, kidney, and brain. Distribution of the chemicals among the organs differed by their log KOW values and generally followed the blood flow path after the gastrointestinal tract. The low contaminant levels found in kidney and brain suggest insufficient time for equilibration into these tissues, especially into the brain where the blood-brain barrier may be slow to traverse. In Lake Apopka fish, dichlorodiphenyltrichloroethanes (DDXs, sum of p,p'-DDE, p,p'-DDD, and p,p'-DDT), Drins (sum of aldrin, dieldrin, and endrin), and hexachlorocyclohexanes (HCHs) were found. For DDXs, the lipid normalized concentrations in each tissue were about the same, as predicted from theory. For Drins and HCHs, the lipid normalized concentrations were similar for kidney, spleen, brain, gonad and muscle, but much lower in the gastrointestinal tract and liver, probably because of metabolism occurring in those tissues. PMID:27394080

  4. The Effect of Dietary Oils of Vegetable Origin on the Performance, Body Composition and Fatty Acid Profiles of Sea Bass (Dicentrarchus labrax L., 1758) Juveniles

    OpenAIRE

    Yildiz, Mustafa; ŞENER, Erdal

    2004-01-01

    Body fat composition and growth performance of sea bass juveniles fed with different kinds of vegetable oils instead of fish oil were studied. Feeding trials were carried out with 600 sea bass (Dicentrarchus labrax L.) juveniles with on initial live weight of 7.58 ± 0.13 g. Experimental feeds contained fish oil, soybean oil, sunflower oil, corn oil and olive oil. In the chemical analyses of the experimental feeds, total crude fat was 12.33 ± 0.24% and total crude protein was 57.42 ± 0.31%. Ac...

  5. Active low frequency sound field control in a listening room using CABS (Controlled Acoustic Bass System) will also reduce the sound transmitted to neighbour rooms

    DEFF Research Database (Denmark)

    Nielsen, Sofus Birkedal; Celestinos, Adrian

    is possible at modal frequencies. For that reason the modal frequencies in the source room will also have big impact on the transmission to neighbour rooms. These low frequency resonance frequencies are very audible in the source room but also in neighbour rooms as a booming bass. CABS (Controlled...... Acoustic Bass System) is a time based room correction system for reproduced sound using loudspeakers. The system can remove room modes at low frequencies, by active cancelling the reflection from at the rear wall to a normal stereo setup. Measurements in a source room using CABS and in two neighbour rooms...

  6. The potential use of legume-based diets supplemented with microbial phytase on the growth performance and feed efficiency of sea bass, Lates calcarifer

    OpenAIRE

    Erlinda S. Ganzon-Naret

    2013-01-01

    A feeding trial was conducted for 12 weeks to evaluate the potential use of legume-based diets supplemented with dietary microbial phytase on the growth performance and feed efficiency of juvenile sea bass, Lates calcarifer. Fifteen sea bass juveniles (mean initial weight of 0.96 g and mean initial total length (TL) of 4.2 cm) were stocked at three replicates into each of the twelve 100 L conical fibreglass tanks containing 90 L sea water in a closed recirculating system with filt...

  7. The potential use of legume-based diets supplemented with microbial phytase on the growth performance and feed efficiency of sea bass, Lates calcarifer

    OpenAIRE

    Erlinda S. Ganzon-Naret

    2013-01-01

    A feeding trial was conducted for 12 weeks to evaluate the potential use of legume-based diets supplemented with dietary microbial phytase on the growth performance and feed efficiency of juvenile sea bass, Lates calcarifer. Fifteen sea bass juveniles (mean initial weight of 0.96 g and mean initial total length (TL) of 4.2 cm) were stocked at three replicates into each of the twelve 100 L conical fibreglass tanks containing 90 L sea water in a closed recirculating system with ...

  8. Influence of dietary vitamin C dosage on turbot (Scophthalmus maximus) and European sea bass (Dicentrarchus labrax) nursery stages

    OpenAIRE

    Merchie, G.; Lavens, P.; Storch, V.; Übel, U.; Nelis, H.; De leenheer, A P; Sorgeloos, P.

    1996-01-01

    Stable forms of vitamin C were verified as dietary sources of ascorbic acid (AA) for the nursery stages of European sea bass and turbot. In a first experiment, various concentrations of ascorbyl palmitate (AP) and one level of AA 2-polyphosphate (ApP) were evaluated in a semi purified diet for European sea bass, Dicentrarchus labrax. A significantly lower AA concentration was detected in the fry fed the AP-supplemented diets compared to the ApP-fed group, providing evidence that AP is an infe...

  9. Evaluation of garlic and onion powder as phyto-additives in the diet of sea bass (Dicentrarcus labrax)

    OpenAIRE

    Norhan E. Saleh; Fady R. Michael; Mohamed M. Toutou

    2015-01-01

    The present study is a preliminary work which was conducted to evaluate the impact of adding 3 levels of garlic (10, 20 and 30 g/kg diet) as well as onion powder (5, 10 and 20 g/kg diet) in a basal diet of sea bass (Dicentrarcus labrax) fry (initial weight = 0.4 ± 0.05 g/fish) on growth performance, feed utilization, body composition, survival rate and some hematological parameters. The present results suggested that dietary garlic or onion powder administrated separately at levels of 30 or 1...

  10. An ovary transcriptome for all maturational stages of the striped bass (Morone saxatilis, a highly advanced perciform fish

    Directory of Open Access Journals (Sweden)

    Reading Benjamin J

    2012-02-01

    Full Text Available Abstract Background The striped bass and its relatives (genus Morone are important fisheries and aquaculture species native to estuaries and rivers of the Atlantic coast and Gulf of Mexico in North America. To open avenues of gene expression research on reproduction and breeding of striped bass, we generated a collection of expressed sequence tags (ESTs from a complementary DNA (cDNA library representative of their ovarian transcriptome. Results Sequences of a total of 230,151 ESTs (51,259,448 bp were acquired by Roche 454 pyrosequencing of cDNA pooled from ovarian tissues obtained at all stages of oocyte growth, at ovulation (eggs, and during preovulatory atresia. Quality filtering of ESTs allowed assembly of 11,208 high-quality contigs ≥ 100 bp, including 2,984 contigs 500 bp or longer (average length 895 bp. Blastx comparisons revealed 5,482 gene orthologues (E-value -3, of which 4,120 (36.7% of total contigs were annotated with Gene Ontology terms (E-value -6. There were 5,726 remaining unknown unique sequences (51.1% of total contigs. All of the high-quality EST sequences are available in the National Center for Biotechnology Information (NCBI Short Read Archive (GenBank: SRX007394. Informative contigs were considered to be abundant if they were assembled from groups of ESTs comprising ≥ 0.15% of the total short read sequences (≥ 345 reads/contig. Approximately 52.5% of these abundant contigs were predicted to have predominant ovary expression through digital differential display in silico comparisons to zebrafish (Danio rerio UniGene orthologues. Over 1,300 Gene Ontology terms from Biological Process classes of Reproduction, Reproductive process, and Developmental process were assigned to this collection of annotated contigs. Conclusions This first large reference sequence database available for the ecologically and economically important temperate basses (genus Morone provides a foundation for gene expression studies in these

  11. Spectre d'absorption de l'hydrogène atomique à très basse pression.

    OpenAIRE

    Moret-Bailly, Jacques

    2015-01-01

    Sous très basse pression, le spectre de l'hydrogène atomique devient un spectre de quasar. Spectra of quasars result primarily from interactions of natural light with atomic hydrogen. A visible absorption of a sharp and saturated spectral line in a gas requires a low pressure, so a long path without blushing as a cosmological redshift. Burbidge and Karlsson observed that redshifts of quasars result from fundamental redshifts, written 3K and 4K, that cause a shift of absorbed beta and gamma...

  12. A study on the efficiency of EFQM versus Bass method: Evidence from national land and housing organization

    Directory of Open Access Journals (Sweden)

    Abolfazl Danaei

    2013-10-01

    Full Text Available Leadership style plays essential role on the success of organizations. The proposed study of this paper compares the efficiency of leadership study based on EFQM and Bass models in national land and housing organization of Iran. The proposed study of this paper designs a questionnaire and distributes it among all managers on this organization. The study covers all different levels of management including middle and top-level management. The results of the survey indicate that the average efficiency of EFQM model is located in 2.7276

  13. The effect of density on sea bass (Dicentrarchus labrax) performance in a tank-based recirculating system

    OpenAIRE

    Sammouth, Sophie; Roque d'Orbcastel, Emmanuelle; Gasset, Eric; Lemarie, Gilles; Breuil, Gilles; Marino, Giovanna; Coeurdacier, Jean-luc; Fivelstad, Sveinung; Blancheton, Jean-Paul

    2009-01-01

    Sea bass (Dicentrarchus tabrax) (135 +/- 4 g) were reared under tank-based recirculating aquaculture system for a 63-day period at four densities: 10, 40, 70, 100 kg m(-3). Fish performance, stress indicators (plasma cortisol, proteonemia plus other blood parameters-Na+, K+, glucose, pH, total CO2- and water quality were monitored. At the end of the 63-day period, resistance to infection was also studied by a nodavirus challenge. A 25-day test was performed on fish from two extreme densities ...

  14. Expression and localization of Aquaporin 1a in the sea-bass (Dicentrarchus labrax during ontogeny

    Directory of Open Access Journals (Sweden)

    IvoneGiffard-Mena

    2011-07-01

    Full Text Available The successful establishment of a species in a given habitat depends on the ability of each of its developing stages to adapt to the environment. In order to understand this process we have studied the adaptation of a euryhaline fish, the sea-bass Dicentrarchus labrax, to various salinities during its ontogeny. The expression and localization of Aquaporin 1a (AQP1a mRNA and protein were determined in different osmoregulatory tissues. In larvae, the sites of AQP1a expression are variable and they shift according to age, implying functional changes. In juveniles after metamorphosis (D32-48 post hatch, 15 - 25 mm and in pre-adults, an increase in AQP1a transcript abundance was noted in the digestive tract, and the AQP1a location was observed in the intestine. In juveniles (D87-100 post hatch, 38 - 48 mm, the transcript levels of AQP1a in the digestive tract and in the kidney were higher in sea water than at lower salinity. These observations, in agreement with existing models, suggest that in sea water-acclimated fish, the imbibed water is absorbed via AQP1a through the digestive tract, particularly the intestine and the rectum. In addition, AQP1a may play a role in water reabsorption in the kidney. These mechanisms compensate dehydratation in sea water, and they contribute to the adaptation of juveniles to salinity changes during sea-lagoon migrations. These results contribute to the interpretation of the adaptation of populations to habitats where salinity varies.

  15. Thickness and Fuel Preheating Effects on Material Flammability in Microgravity from the BASS Experiment

    Science.gov (United States)

    Ferkul, Paul V.; Olson, Sandra L.; Takahashi, Fumiaki; Endo, Makoto; Johnson, Michael C.; T'ien, James S.

    2013-01-01

    The Burning and Suppression of Solids (BASS) experiment was performed on the International Space Station. Microgravity combustion tests burning thin and thick flat samples, acrylic spheres, and candles were conducted. The samples were mounted inside a small wind tunnel which could impose air flow speeds up to 40 cms. The wind tunnel was installed in the Microgravity Science Glovebox which supplied power, imaging, and a level of containment. The effects of air flow speed, fuel thickness, fuel preheating, and nitrogen dilution on flame appearance, flame growth, and spread rates were determined in both the opposed and concurrent flow configuration. In some cases, a jet of nitrogen was introduced to attempt to extinguish the flame. Microgravity flames were found to be especially sensitive to air flow speed in the range 0 to 5 cms. The gas phase response is much faster compared to the solid and so as the flow speed is changed, the flame responds with almost no delay. At the lowest speeds examined (less than 1 cms) all the flames tended to become dim blue and very stable. However, heat loss at these very low convective rates is small so the flames can burn for a long time. At moderate flow speeds (between about 1 and 5 cms) the flame continually heats the solid fuel resulting in an increasing fuel temperature, higher rate of fuel vaporization, and a stronger, more luminous flame as time progresses. Only the smallest flames burning acrylic slabs appeared to be adversely influenced by solid conductive heat loss, but even these burned for over 5 minutes before self-extinguishing. This has implications for spacecraft fire safety since a tiny flame might be undetected for a long time. While the small flame is not particularly hazardous if it remains small, the danger is that it might flare up if the air convection is suddenly increased or if the flame spreads into another fuel source.

  16. Uso tradicional y actual de spirulina sp. (Arthrospira sp.)

    OpenAIRE

    Liliana Ramírez- Moreno; Roxana Olvera-Ramírez

    2006-01-01

    Spirulina sp. (Arthrospira sp.) es una cianobacteria filamentosa no diferenciada, habitante de lagos alcalinos, que se cultiva para consumo humano debido a su contenido nutricional. En México, el consumo de esta cianobacteria se remonta a tiempos prehispánicos, cuando era conocida como tecuitlatl, siendo conocida como dihé por las tribus nativas de la región del lago de Chad, en África. En los últimos años se le han atribuido diversos efectos positivos en el tratamiento de algunos tipos de al...

  17. Seismic tomography of Basse-Terre volcanic island, Guadeloupe, Lesser Antilles, using earthquake travel times and noise correlations

    Science.gov (United States)

    Barnoud, Anne; Coutant, Olivier; Bouligand, Claire; Massin, Frédérick; Stehly, Laurent

    2015-04-01

    We image the volcanic island of Basse-Terre, Guadeloupe, Lesser Antilles, using both earthquake travel times and noise correlations. (1) A new earthquake catalog was recently compiled for the Lesser Antilles by the CDSA/OVSG/IPGP (Massin et al., EGU General Assembly 2014) and allows us to perform classical travel time tomography to obtain smooth 3D body wave velocity models. The geometrical configuration of the volcanic arc controls the resolution of the model in our zone of interest. (2) Surface wave tomography using noise correlations was successfully applied to volcanoes (Brenguier et al., Geophys. Res. Lett. 2007). We use seismic noise recorded at 16 broad-band stations and 9 short-period stations from Basse-Terre over a period of six years (2007-2012). For each station pair, we extract a dispersion curve from the noise correlation to get surface wave velocity models. The inversion of the dispersion curves produces a 3D S-wave velocity model of the island. The spatial distribution of seismic stations accross the island is highly heterogeneous, leading to higher resolution near the dome of the Soufrière of Guadeloupe volcano. Resulting velocity models are compared with densities obtained by 3D inversion of gravimetric data (Barnoud et al., AGU Fall Meeting 2013). Further work should include simultaneous inversion of seismic and gravimetric datasets to overcome resolution limitations.

  18. Administration of a Polyphenol-Enriched Feed to Farmed Sea Bass (Dicentrarchus labrax L. Modulates Intestinal and Spleen Immune Responses

    Directory of Open Access Journals (Sweden)

    Thea Magrone

    2016-01-01

    Full Text Available Farmed fish are exposed to a continuous antigenic pressure by microbial and environmental agents, which may lead to a condition of chronic inflammation. In view of the notion that polyphenols, largely contained in fruits and vegetables, are endowed with antioxidant and anti-inflammatory activities, farmed sea bass (Dicentrarchus labrax L. have been administered with red grape polyphenol-enriched feed. Polyphenols were extracted from the seeds of Canosina Nero di Troia Vitis vinifera and mixed with conventional feed at two different concentrations (100 and 200 mg/kg, resp.. Fish samples collected at days 223 and 273, respectively, were evaluated for intestinal and spleen cytokine release as well as for spleen macrophage (MØ and melanomacrophage center (MMC areas and distribution. Data will show that in treated fish decrease of intestinal interleukin- (IL- 1β and IL-6 and increase of splenic interferon- (IFN- γ occur. On the other hand, in the spleen reduction of MØ number seems to parallel increase in MMCs. Collectively, these data suggest that polyphenol-administered sea bass generate lower levels of intestinal proinflammatory cytokines, while producing larger amounts of spleen IFN-γ, as an expression of a robust and protective adaptive immune response. Increase of MMCs corroborates the evidence for a protective spleen response induced by feed enriched with polyphenols.

  19. Administration of a Polyphenol-Enriched Feed to Farmed Sea Bass (Dicentrarchus labrax L.) Modulates Intestinal and Spleen Immune Responses.

    Science.gov (United States)

    Magrone, Thea; Fontana, Sergio; Laforgia, Flavia; Dragone, Teresa; Jirillo, Emilio; Passantino, Letizia

    2016-01-01

    Farmed fish are exposed to a continuous antigenic pressure by microbial and environmental agents, which may lead to a condition of chronic inflammation. In view of the notion that polyphenols, largely contained in fruits and vegetables, are endowed with antioxidant and anti-inflammatory activities, farmed sea bass (Dicentrarchus labrax L.) have been administered with red grape polyphenol-enriched feed. Polyphenols were extracted from the seeds of Canosina Nero di Troia Vitis vinifera and mixed with conventional feed at two different concentrations (100 and 200 mg/kg, resp.). Fish samples collected at days 223 and 273, respectively, were evaluated for intestinal and spleen cytokine release as well as for spleen macrophage (MØ) and melanomacrophage center (MMC) areas and distribution. Data will show that in treated fish decrease of intestinal interleukin- (IL-) 1β and IL-6 and increase of splenic interferon- (IFN-) γ occur. On the other hand, in the spleen reduction of MØ number seems to parallel increase in MMCs. Collectively, these data suggest that polyphenol-administered sea bass generate lower levels of intestinal proinflammatory cytokines, while producing larger amounts of spleen IFN-γ, as an expression of a robust and protective adaptive immune response. Increase of MMCs corroborates the evidence for a protective spleen response induced by feed enriched with polyphenols. PMID:26779301

  20. BANYAN. VII. A New Population of Young Substellar Candidate Members of Nearby Moving Groups from the BASS Survey

    CERN Document Server

    Gagné, Jonathan; Cruz, Kelle L; Lafrenière, David; Doyon, René; Malo, Lison; Burgasser, Adam J; Naud, Marie-Eve; Artigau, Étienne; Bouchard, Sandie; Gizis, John E; Albert, Loïc

    2015-01-01

    [Abbreviated] We present the results of a near-infrared (NIR) spectroscopic follow-up survey of 182 M4-L7 low-mass stars and brown dwarfs (BDs) from the BANYAN All-Sky Survey (BASS) for candidate members of nearby, young moving groups (YMGs). We confirm signs of low-gravity for 42 new BD discoveries with estimated masses between 8-75 $M_{Jup}$ and identify previously unrecognized signs of low gravity for 24 known BDs. This allows us to refine the fraction of low-gravity dwarfs in the high-probability BASS sample to $\\sim$82%. We use this unique sample of 66 young BDs, supplemented with 22 young BDs from the literature, to construct new empirical NIR absolute magnitude and color sequences for low-gravity BDs. We obtain a spectroscopic confirmation of low-gravity for 2MASS J14252798-3650229, which is a new $\\sim$27 $M_{Jup}$, L4 $\\gamma$ bona fide member of AB Doradus. We identify a total of 19 new low-gravity candidate members of YMGs with estimated masses below 13 $M_{Jup}$, seven of which have kinematically ...

  1. Growth parameters in European sea bass (Dicentrarchus labraxL.: effects of live weight and water temperature

    Directory of Open Access Journals (Sweden)

    Rodolfo Ballestrazzi

    2010-01-01

    Full Text Available The voluntary feed intake (VFI and growth rate of sea bass were studied for 147 days, based on different starting live  weights, under natural photoperiods and varied water temperatures. Sea bass (n = 720 were divided into five weight  classes (60-70, 90-110, 130-150, 160-180 and 230-250 g and distributed among 20 tanks. Seven different water tem-  peratures were compared: 13, 16, 19, 22, 25, 28 and 31 °C. A commercial extruded diet (N x 6.25, 43.7% DM; crude  fat, 25.7% DM was used. The trial was performed in a closed-circuit plant, with a daily water turnover rate of 10%. Each  tank was fitted with an apparatus for collecting the uneaten food. Automatic feeders distributed equal amounts of food  for ten meals per day. The daily food allowance was adjusted, based on fish biomass, to permit ad libitum feeding and  to leave approximately 20% uneaten. Fish were exposed to natural photoperiod (March-July. Non-linear regression  equations were used to calculate the best-fitting curves for the data. 

  2. The characteristics of vasa gene from Japanese sea bass ( Lateolabrax japonicas) and its response to the external hormones

    Science.gov (United States)

    Chi, Meili; Wen, Haishen; Ni, Meng; Qian, Kun; Zhang, Pei; Chai, Senhao

    2015-08-01

    The RNA helicase Vasa is an important regulator of primordial germ cell development. Its function in mature fish, especially the hormone-related differences in maturing male fish has seldom been documented. In this study, a full length cDNA sequence of the vasa gene was cloned from Japanese sea bass, Lateolabrax japonicas, and it was named jsb-vasa. Homology analysis showed that jsb-vasa was closely related to its teleost homologs. The spatial distribution of jsb-vasa indicated that it was only highly expressed in testis, showing its germ cell-specific expression pattern. During the testicular development cycle, jsb-vasa was highly expressed during early period of spermatogenesis, and reduced when spermatogenesis advanced. In addition, the jsb-vasa gene expression was significantly inhibited at 6 h, 12 h and 24 h after injecting hCG (human chorionic gonadotropin) and GnRHa (Gonadotropin-releasing hormone analogue), indicating that jsb-vasa gene may play an important role in spermatogenesis of Japanese sea bass, and be under the regulation of external sex hormones.

  3. Intraspecific individual variation of temperature tolerance associated with oxygen demand in the European sea bass (Dicentrarchus labrax).

    Science.gov (United States)

    Ozolina, Karlina; Shiels, Holly A; Ollivier, Hélène; Claireaux, Guy

    2016-01-01

    The European sea bass (Dicentrarchus labrax) is an economically important fish native to the Mediterranean and Northern Atlantic. Its complex life cycle involves many migrations through temperature gradients that affect the energetic demands of swimming. Previous studies have shown large intraspecific variation in swimming performance and temperature tolerance, which could include deleterious and advantageous traits under the evolutionary pressure of climate change. However, little is known of the underlying determinants of this individual variation. We investigated individual variation in temperature tolerance in 30 sea bass by exposing them to a warm temperature challenge test. The eight most temperature-tolerant and eight most temperature-sensitive fish were then studied further to determine maximal swimming speed (U CAT), aerobic scope and post-exercise oxygen consumption. Finally, ventricular contractility in each group was determined using isometric muscle preparations. The temperature-tolerant fish showed lower resting oxygen consumption rates, possessed larger hearts and initially recovered from exhaustive exercise faster than the temperature-sensitive fish. Thus, whole-animal temperature tolerance was associated with important performance traits. However, the temperature-tolerant fish also demonstrated poorer maximal swimming capacity (i.e. lower U CAT) than their temperature-sensitive counterparts, which may indicate a trade-off between temperature tolerance and swimming performance. Interestingly, the larger relative ventricular mass of the temperature-tolerant fish did not equate to greater ventricular contractility, suggesting that larger stroke volumes, rather than greater contractile strength, may be associated with thermal tolerance in this species. PMID:27382468

  4. Liver proteome response of largemouth bass (Micropterus salmoides) exposed to several environmental contaminants: Potential insights into biomarker development

    International Nuclear Information System (INIS)

    Liver proteome response of largemouth bass (Micropterus salmoides) exposed to environmental contaminants was analyzed to identify novel biomarkers of exposure. Adult male bass were exposed to cadmium chloride (CdCl2), atrazine, PCB 126, phenanthrene, or toxaphene via intraperitoneal injection with target body burdens of 0.00067, 3.0, 2.5, 50, and 100 μg/g, respectively. After a 96 h exposure, hepatic proteins were separated with two-dimensional gel electrophoresis and differentially expressed proteins (vs. controls) recognized and identified with MALDI-TOF/TOF mass spectrometry. We identified, 30, 18, eight, 19, and five proteins as differentially expressed within the CdCl2, atrazine, PCB 126, phenanthrene, and toxaphene treatments, respectively. Alterations were observed in the expression of proteins associated with cellular ion homeostasis (toxaphene), oxidative stress (phenanthrene, PCB 126), and energy production including glycolysis (CdCl2, atrazine) and ATP synthesis (atrazine). This work supports the further evaluation of several of these proteins as biomarkers of contaminant exposure in fish.

  5. Estimated loss of juvenile salmonids to predation by northern squawfish, walleyes, and smallmouth bass in John Day Reservoir, Columbia River

    International Nuclear Information System (INIS)

    The authors estimated the loss of juvenile salmonids Oncorhynchus spp. to predation by northern squawfish Ptychocheilus oregonensis, walleyes Stizostedion vitreum, and smallmouth bass Micropterus dolomieu in John Day Reservoir during 1983-1986. Their estimates were based on measures of daily prey consumption, predator numbers, and numbers of juvenile salmonids entering the reservoir during the April-August period of migration. They estimated the mean annual loss was 2.7 million juvenile salmonids. Northern squawfish were responsible for 78% of the total loss; walleyes accounted for 13% and smallmouth bass for 9%. Twenty-one percent of the loss occurred in a small area immediately below McNary Dam at the head of John Day Reservoir. The authors estimated that the three predator species consumed 14% of all juvenile salmonids that entered the reservoir. Mortality changed by month and increased late in the migration season. Monthly mortality estimates ranged from 7% in June and 61% in August. Mortality from predation was highest for chinook salmon O. tshawytscha, which migrated in July and August. Despite uncertainties in the estimates, it is clear that predation by resident fish predators can easily account for previously explained mortality of out-migrating juvenile salmonids. Alteration of the Columbia River by dams and a decline in the number of salmonids could have increased the fraction of mortality caused by predation over what is was in the past

  6. Evaluation of garlic and onion powder as phyto-additives in the diet of sea bass (Dicentrarcus labrax

    Directory of Open Access Journals (Sweden)

    Norhan E. Saleh

    2015-01-01

    Full Text Available The present study is a preliminary work which was conducted to evaluate the impact of adding 3 levels of garlic (10, 20 and 30 g/kg diet as well as onion powder (5, 10 and 20 g/kg diet in a basal diet of sea bass (Dicentrarcus labrax fry (initial weight = 0.4 ± 0.05 g/fish on growth performance, feed utilization, body composition, survival rate and some hematological parameters. The present results suggested that dietary garlic or onion powder administrated separately at levels of 30 or 10 g/kg respectively could improve survival, growth and feed utilization of sea bass fry. Administration of garlic and onion indicates a slide edge for garlic to onion in growth, feed utilization and fish protein content. It will also induce a significant increase in some blood variables (hemoglobin content, hematocrit value, mean corpuscular volume, mean corpuscular hemoglobin and leukocytes count in treated fish when compared to the control fish group.

  7. The effect of the dye Aquashade(c) on water quality, phytoplankton, zooplankton, and sunshine bass fingerling production in fertilized culture ponds

    Science.gov (United States)

    Rooted macrophytes make harvesting fingerling sunshine bass from ponds difficult because they can smother fish or tangle in the seines and increase fish stress by increasing time needed to retrieve the fingerlings from the net. Aquashade® is a dye that is used to reduce macrophyte growth by reducin...

  8. FINGERLING CULTURE OF SUNSHINE BASS MORONE CHRYSOPS X M. SAXATILIS IN TANKS WITH ALGAE PASTE-FED ROTIFERS, ARTEMIA NAUPLII AND MICROENCAPSULATED FEED AT THREE CONCENTRATIONS

    Science.gov (United States)

    Sunshine bass fingerling production occurs almost exclusively in ponds. To increase production and to maintain year-round production in temperate climates indoor tank culture is required. While tank production of fingerlings has been demonstrated, little is known about feeding requirements. Sunshi...

  9. SeaBASS Bio-optical and pigment data collected from 1979-08-22 to 2011-12-14 (NCEI Accession 0086308)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This accession contains SeaWiFS Bio-optical Archive and Storage System (SeaBASS) bio-optical, pigment, and other data collected from 1979-08-22 to 2011-12-14....

  10. Computer simulation model for the striped bass young-of-the-year population in the Hudson River. [Effects of entrainment and impingement at power plants on population dynamics

    Energy Technology Data Exchange (ETDEWEB)

    Eraslan, A.H.; Van Winkle, W.; Sharp, R.D.; Christensen, S.W.; Goodyear, C.P.; Rush, R.M.; Fulkerson, W.

    1975-09-01

    This report presents a daily transient (tidal-averaged), longitudinally one-dimensional (cross-section-averaged) computer simulation model for the assessment of the entrainment and impingement impacts of power plant operations on young-of-the-year populations of the striped bass, Morone saxatilis, in the Hudson River.

  11. Effects of using slurry ice during transportation on the microbiological, chemical, and sensory assessments of aquacultured sea bass (Dicentrarchus labrax) stored at 4 degrees C.

    Science.gov (United States)

    Cakli, Sukran; Kilinc, Berna; Dincer, Tolga; Tolasa, Sebnem

    2006-01-01

    Slurry ice, a biphasic system consisting of small spherical ice crystals surrounded by seawater at subzero temperature, was evaluated as a new chilled storage method for whole sea bass (Dicentrarchus labrax) a sparidae fish species of remarkable commercial interests. In this study two different group of chilling methods were used during transportation; in slurry ice packaged (Group A), and flake ice packaged (Group B). The effect of this advanced system during transportation on quality losses and the shelf life of aquacultured sea bass was evaluated. Mesophilic counts for sea bass exceeded 7 log cfu/g, which is considered the maximum level for acceptability for freshwater and marine fish after 13 days for groups A and B. On day 13 TVB-N values of groups A and B, reached the legal limits (35 mg/100 g set for TVB-N) for consumption. According to the results of sensory analyses, up to day 9 all the groups were determined as "acceptable" but on day 13 the groups A and B were no longer acceptable. The main negative aspect related to quality loss in slurry ice group corresponded to the appearance of eyes and gills. Using slurry ice during transportation did not extend the shelf life of sea bass stored at 4 degrees C. PMID:16864138

  12. Identification of centrarchid hepcidins and evidence that 17β-estradiol disrupts constitutive expression of hepcidin-1 and inducible expression of hepcidin-2 in largemouth bass (Micropterus salmoides)

    Science.gov (United States)

    Robertson, L.S.; Iwanowicz, L.R.; Marranca, J.M.

    2009-01-01

    Hepcidin is a highly conserved antimicrobial peptide and iron-regulatory hormone. Here, we identify two hepcidin genes (hep-1 and hep-2) in largemouth bass (Micropterus salmoides) and smallmouth bass (Micropterus dolomieu). Hepcidin-1 contains a putative ATCUN metal-binding site in the amino-terminus that is missing in hepcidin-2, suggesting that hepcidin-1 may function as an iron-regulatory hormone. Both hepcidins are predominately expressed in the liver of largemouth bass, similar to other fish and mammals. Experimental exposure of pond-raised largemouth bass to 17β-estradiol and/or the bacteria Edwardsiella ictaluri led to distinct changes in expression of hep-1 and hep-2. Estradiol reduced the constitutive expression of hep-1 in the liver. Bacterial exposure induced expression of hep-2, suggesting that hepcidin-2 may have an antimicrobial function, and this induction was abolished by estradiol. To our knowledge, this is the first report of the regulation of hepcidin expression by estradiol in either fish or mammals.

  13. The effects of menhaden oil, flaxseed oil and a dairy-yeast prebiotic on growth , health , feed conversion , survival , critical maximum temperature, and body composition of sunshine bass

    Science.gov (United States)

    A 12-week feeding trial was conducted with juvenile "sunshine bass" female Morone chrysops x male M. saxatilis using four diets containing two levels of a dairy-yeast prebiotic (0 or 2%) and either menhaden fish oil or flaxseed oil as a lipid source. The objective was to determine the main and inte...

  14. Fish consumption and risk of contamination by mercury – Considerations on the definition of edible parts based on the case study of European sea bass

    International Nuclear Information System (INIS)

    Highlights: ► We estimated the risk to humans trough ingestion of sea bass contaminated with Hg. ► Total and organic Hg in muscle exceeded weekly and daily safety limits. ► There’s risk through ingestion although Hg was below the maximum limit set by EU. ► The ingestion of European sea bass contaminated with Hg poses risk to human health. ► Other organs rather than muscle should be considered as edible tissue. - Abstract: In the present study, the risk to humans by consuming European sea bass (Dicentrarchus labrax), captured at three sites along a Hg contamination gradient, was evaluated by comparing muscle and kidney total Hg (T-Hg) levels with the European regulations for marketed fish. Moreover, T-Hg and organic Hg (O-Hg) levels in muscle were compared with the Provisional Tolerable Weekly Intake (PTWI) and the Reference Dose (RfD). Although T-Hg levels in muscle were below the European value allowable for marketed fish, kidney’s levels were higher than the set value, stressing the importance of redefining the concept of edible tissue and which tissues should be considered. Mercury weekly ingestion in the contaminated areas was higher than the PTWI, and O-Hg daily ingestion rates were higher than the RfD in all sampling sites. Thus, populations consuming sea bass from the contaminated sites may be at risk, with particular relevance for children and pregnant women.

  15. Influence of reproductive status sex hormones and temperature on plasma IGF-I concentrations in sunshine bass (Morone Chrysops X Morone Saxatilis)

    Science.gov (United States)

    Insulin-like growth factor-I (IGF-I) concentrations in male and female sunshine bass (Morone chrysops X Morone saxatilis) were determined in March, early April, and late April in outdoor ponds at a commercial farm. Female fish were always larger than male fish, however plasma IGF-I concentrations w...

  16. Feeding transition of cultured largemouth bass (Micropterus salmoides, Lacépede, 1802) from an artificial pelleted feed to live prey

    Czech Academy of Sciences Publication Activity Database

    Frouzová, Jaroslava; Porak, W. F.; Johnson, W. E.

    2013-01-01

    Roč. 29, č. 6 (2013), s. 1364-1366. ISSN 0175-8659 R&D Projects: GA MZe(CZ) QH81046 Institutional support: RVO:60077344 Keywords : largemouth bass * feeding behavior * novel prey Subject RIV: GL - Fishing Impact factor: 0.903, year: 2013

  17. Comparison of two different fecal collection methods for protein digestibility and amino acid availability coefficients of three animal protein sources for sunshine bass (Morone chrysops x Morone saxatilis)

    Science.gov (United States)

    Apparent digestibility coefficients (ADCs) for protein and individual amino acid availabilities in menhaden fish meal (MEN) and two grades of poultry by-product meal (PBM) were determined for market-size (500 g) sunshine bass using two different fecal collection methods, passive netting (net) or man...

  18. Hepatic transcriptomic and metabolic responses of hybrid striped bass (Morone saxatilis×Morone chrysops) to acute and chronic hypoxic insult.

    Science.gov (United States)

    Beck, Benjamin H; Fuller, S Adam; Li, Chao; Green, Bartholomew W; Zhao, Honggang; Rawles, Steven D; Webster, Carl D; Peatman, Eric

    2016-06-01

    Striped bass (Morone saxatilis), white bass (Morone chrysops), and their hybrid are an important group of fish prized for recreational angling in the United States, and there and abroad as a high-value farmed fish. Regardless of habitat, it is not uncommon for fish of the genus Morone to encounter and cope with conditions of scarce oxygen availability. Previously, we determined that hybrid striped bass reared under conditions of chronic hypoxia exhibited reduced feed intake, lower lipid and nutrient retention, and poor growth. To better understand the molecular mechanisms governing these phenotypes, in the present study, we examined the transcriptomic profiles of hepatic tissue in hybrid striped bass exposed to chronic hypoxia (90days at 25% oxygen saturation) and acute hypoxia (6h at 25% oxygen saturation). Using high-throughput RNA-seq, we found that over 1400 genes were differentially expressed under disparate oxygen conditions, with the vast majority of transcriptional changes occurring in the acute hypoxia treatment. Gene pathway and bioenergetics analyses revealed hypoxia-mediated perturbation of genes and gene networks related to lipid metabolism, cell death, and changes in hepatic mitochondrial content and cellular respiration. This study offers a more comprehensive view of the temporal and tissue-specific transcriptional changes that occur during hypoxia, and reveals new and shared mechanisms of hypoxia tolerance in teleosts. PMID:26851735

  19. Metabolism of the polycyclic musk galaxolide and its interference with endogenous and xenobiotic metabolizing enzymes in the European sea bass (Dicentrarchus labrax)

    International Nuclear Information System (INIS)

    This study investigates the metabolism and mode of action of galaxolide (HHCB) in the European sea bass -Dicentrarchus labrax- following a single intraperitoneal injection of 50 mg HHCB/kg body weight. In addition, a group of fish was injected with 50 mg/kg of ketoconazole (KCZ), a fungicide that is known to interfere with different Cyp isoenzymes. HHCB was actively metabolised by sea bass and acted as a weak inhibitor of the synthesis of oxyandrogens in gonads of male fish. Both, HHCB and a hydroxylated metabolite were detected in bile. The fungicide ketoconazole was a strong inhibitor of Cyp11β and Cyp3a-catalyzed activities. The work contributes to the better understanding of the impact of synthetic musks on fish and proposes the determination of HHCB and/or its hydroxylated metabolite in bile as a tool to assess environmental exposure in wild fish. -- Highlights: ► The metabolism and mode of action of galaxolide has been investigated in sea bass. ► A hydroxylated metabolite was for the first time identified in fish bile. ► EROD and BCOD activities were not altered by galaxolide exposure. ► Galaxolide decreased moderately the synthesis of oxyandrogens in testes. -- HHCB is actively metabolised by sea bass and acts as a weak inhibitor of the synthesis of oxyandrogens in gonads of male fish

  20. A Review. The Use of the Alto, Bass and Contrabass Clarinets in Selected Wind Band Compositions Written Between 1951 and 1972.

    Science.gov (United States)

    Heim, Norman M.

    1987-01-01

    Reviews a dissertation which examined the scoring practices for alto, bass, and contrabass clarinets in selected compositions written between 1951-1972. The study identified new practices for scoring low clarinets in wind band compositions. The study is recommended to readers seeking a good understanding of the use of clarinets in these…

  1. Assessment of Aquaflor (c), copper sulfate and potassium permanganate for control of Aeromonas hydrophila and Flavobacterium columnare infection in sunshine bass, Morone chrysops female x Morone saxatilis male

    Science.gov (United States)

    Two experiments were conducted to assess different therapeutants against a mixed infection of Aeromonas hydrophila and Flavobacterium columnare in sunshine bass (SB) (Morone chrysops female x Morone saxatilis male). Experiment 1 assessed the efficacy of copper sulfate (CuSO4), florfenicol-medicated...

  2. Lysine supplementation of commercial fishmeal-free diet in hybrid striped bass Morone chrysops x M. saxatilis affects expression of growth related genes

    Science.gov (United States)

    Our recent results in hybrid striped bass (HSB) concluded that ideal protein theory accurately predicts first-limiting amino acids in commercial diet formulations if accurate amino acid availability data are used and that appropriate levels of supplemental lysine are needed in order to improve fish ...

  3. Gene networks and toxicity pathways induced by acute cadmium exposure in adult largemouth bass (Micropterus salmoides)

    Energy Technology Data Exchange (ETDEWEB)

    Mehinto, Alvine C., E-mail: alvinam@sccwrp.org [Southern California Coastal Water Research Project, Costa Mesa, CA 92626 (United States); Department of Physiological Sciences and Center for Environmental and Human Toxicology, University of Florida, Gainesville, FL 32611 (United States); Prucha, Melinda S. [Department of Human Genetics, Yerkes National Primate Research Center, Emory University, Atlanta, GA 30322 (United States); Department of Physiological Sciences and Center for Environmental and Human Toxicology, University of Florida, Gainesville, FL 32611 (United States); Colli-Dula, Reyna C.; Kroll, Kevin J.; Lavelle, Candice M.; Barber, David S. [Department of Physiological Sciences and Center for Environmental and Human Toxicology, University of Florida, Gainesville, FL 32611 (United States); Vulpe, Christopher D. [Department of Nutritional Sciences and Toxicology, University of California, Berkeley, CA 94720 (United States); Denslow, Nancy D. [Department of Physiological Sciences and Center for Environmental and Human Toxicology, University of Florida, Gainesville, FL 32611 (United States)

    2014-07-01

    Highlights: • Low-level acute cadmium exposure elicited tissue-specific gene expression changes. • Molecular initiating events included oxidative stress and disruption of DNA repair. • Metallothionein, a marker of metal exposure, was not significantly affected. • We report effects of cadmium on cholesterol metabolism and steroid synthesis. • Diabetic complications and impaired reproduction are potential adverse outcomes. - Abstract: Cadmium is a heavy metal that can accumulate to toxic levels in the environment leading to detrimental effects in animals and humans including kidney, liver and lung injuries. Using a transcriptomics approach, genes and cellular pathways affected by a low dose of cadmium were investigated. Adult largemouth bass were intraperitoneally injected with 20 μg/kg of cadmium chloride (mean exposure level – 2.6 μg of cadmium per fish) and microarray analyses were conducted in the liver and testis 48 h after injection. Transcriptomic profiles identified in response to cadmium exposure were tissue-specific with the most differential expression changes found in the liver tissues, which also contained much higher levels of cadmium than the testis. Acute exposure to a low dose of cadmium induced oxidative stress response and oxidative damage pathways in the liver. The mRNA levels of antioxidants such as catalase increased and numerous transcripts related to DNA damage and DNA repair were significantly altered. Hepatic mRNA levels of metallothionein, a molecular marker of metal exposure, did not increase significantly after 48 h exposure. Carbohydrate metabolic pathways were also disrupted with hepatic transcripts such as UDP-glucose, pyrophosphorylase 2, and sorbitol dehydrogenase highly induced. Both tissues exhibited a disruption of steroid signaling pathways. In the testis, estrogen receptor beta and transcripts linked to cholesterol metabolism were suppressed. On the contrary, genes involved in cholesterol metabolism were highly

  4. Gene networks and toxicity pathways induced by acute cadmium exposure in adult largemouth bass (Micropterus salmoides)

    International Nuclear Information System (INIS)

    Highlights: • Low-level acute cadmium exposure elicited tissue-specific gene expression changes. • Molecular initiating events included oxidative stress and disruption of DNA repair. • Metallothionein, a marker of metal exposure, was not significantly affected. • We report effects of cadmium on cholesterol metabolism and steroid synthesis. • Diabetic complications and impaired reproduction are potential adverse outcomes. - Abstract: Cadmium is a heavy metal that can accumulate to toxic levels in the environment leading to detrimental effects in animals and humans including kidney, liver and lung injuries. Using a transcriptomics approach, genes and cellular pathways affected by a low dose of cadmium were investigated. Adult largemouth bass were intraperitoneally injected with 20 μg/kg of cadmium chloride (mean exposure level – 2.6 μg of cadmium per fish) and microarray analyses were conducted in the liver and testis 48 h after injection. Transcriptomic profiles identified in response to cadmium exposure were tissue-specific with the most differential expression changes found in the liver tissues, which also contained much higher levels of cadmium than the testis. Acute exposure to a low dose of cadmium induced oxidative stress response and oxidative damage pathways in the liver. The mRNA levels of antioxidants such as catalase increased and numerous transcripts related to DNA damage and DNA repair were significantly altered. Hepatic mRNA levels of metallothionein, a molecular marker of metal exposure, did not increase significantly after 48 h exposure. Carbohydrate metabolic pathways were also disrupted with hepatic transcripts such as UDP-glucose, pyrophosphorylase 2, and sorbitol dehydrogenase highly induced. Both tissues exhibited a disruption of steroid signaling pathways. In the testis, estrogen receptor beta and transcripts linked to cholesterol metabolism were suppressed. On the contrary, genes involved in cholesterol metabolism were highly

  5. Reproductive health of bass in the potomac, USA, drainage: Part 1. exploring the effects of proximity to wastewater treatment plant discharge

    Science.gov (United States)

    Iwanowicz, L.R.; Blazer, V.S.; Guy, C.P.; Pinkney, A.E.; Mullcan, J.E.; Alvarezw, D.A.

    2009-01-01

    Abstract-Intersex (specifically, testicular oocytes) has been observed in male smalimouth bass (SMB; Micropterus dolomieu) and other centrarchids in the South Branch of the Potomac River, USA, and forks of the Shenandoah River, USA. during the past five years. This condition often is associated with exposure to estrogenic endocrine-disrupting chemicals in some fish species, but such chemicals and their sources have yet to be identified in the Potomac. In an attempt to better understand the plausible causes of this condition, we investigated the reproductive health of bass sampled up- and downstream of wastewater treatment plant (WWTP) effluent point sources on the Potomac River in Maryland, USA. Smallmouth bass were sampled from the Conococheague Creek and the Monocacy River, and largemouth bass (LMB; Micropterus salmoides) were collected near the Blue Plains WWTP on the mainstem of the Potomac River. Chemical analyses of compounds captured in passive samplers at these locations also were conducted. A high prevalence of intersex (82-l00%) was identified in male SMB at all sites regardless of collection area. A lower prevalence of intersex (23%) was identified in male LMB collected at the Blue Plains site. When up- and downstream fish were compared, significant differences were noted only in fish from the Conococheague. Differences included condition factor, gonadosomatic index, plasma vitellogenin concentration, and estrogen to testosterone ratio. In general, chemicals associated with wastewater effluent, storm-water runoff, and agriculture were more prevalent at the downstream sampling sites. An exception was atrazine and its associated metabolites, which were present in greater concentrations at the upstream sites. It appears that proximity to effluent from WWTPs may influence the reproductive health of bass in the Potomac watershed, but inputs from other sources likely contribute to the widespread, high incidence of testicular oocytes. ?? 2009 SETAC.

  6. A biorobotic model of the suction-feeding system in largemouth bass: the roles of motor program speed and hyoid kinematics.

    Science.gov (United States)

    Kenaley, Christopher P; Lauder, George V

    2016-07-01

    The vast majority of ray-finned fishes capture prey through suction feeding. The basis of this behavior is the generation of subambient pressure through rapid expansion of a highly kinetic skull. Over the last four decades, results from in vivo experiments have elucidated the general relationships between morphological parameters and subambient pressure generation. Until now, however, researchers have been unable to tease apart the discrete contributions of, and complex relationships among, the musculoskeletal elements that support buccal expansion. Fortunately, over the last decade, biorobotic models have gained a foothold in comparative research and show great promise in addressing long-standing questions in vertebrate biomechanics. In this paper, we present BassBot, a biorobotic model of the head of the largemouth bass (Micropterus salmoides). BassBot incorporates a 3D acrylic plastic armature of the neurocranium, maxillary apparatus, lower jaw, hyoid, suspensorium and opercular apparatus. Programming of linear motors permits precise reproduction of live kinematic behaviors including hyoid depression and rotation, premaxillary protrusion, and lateral expansion of the suspensoria. BassBot reproduced faithful kinematic and pressure dynamics relative to live bass. We show that motor program speed has a direct relationship to subambient pressure generation. Like vertebrate muscle, the linear motors that powered kinematics were able to produce larger magnitudes of force at slower velocities and, thus, were able to accelerate linkages more quickly and generate larger magnitudes of subambient pressure. In addition, we demonstrate that disrupting the kinematic behavior of the hyoid interferes with the anterior-to-posterior expansion gradient. This resulted in a significant reduction in subambient pressure generation and pressure impulse of 51% and 64%, respectively. These results reveal the promise biorobotic models have for isolating individual parameters and assessing

  7. Multiple vitellogenins and product yolk proteins in European sea bass (Dicentrarchus labrax): Molecular characterization, quantification in plasma, liver and ovary, and maturational proteolysis.

    Science.gov (United States)

    Yilmaz, Ozlem; Prat, Francisco; Ibáñez, A Jose; Köksoy, Sadi; Amano, Haruna; Sullivan, Craig V

    2016-01-01

    Three complete vitellogenin (Vtg) polypeptides of European sea bass (Dicentrarchus labrax), an acanthomorph teleost spawning pelagic eggs in seawater, were deduced from cDNA and identified as VtgAa, VtgAb and VtgC based on current Vtg nomenclature and phylogeny. Label free quantitative mass spectrometry verified the presence of the three sea bass Vtgs or their product yolk proteins (YPs) in liver, plasma and ovary of postvitellogenic females. As evidenced by normalized spectral counts, VtgAb-derived protein was 2- to 5-fold more abundant, depending on sample type, than for VtgAa, while VtgC-derived protein was less abundant, albeit only 3-fold lower than for VtgAb in the ovary. Western blotting with Vtg type-specific antisera raised against corresponding gray mullet (Mugil cephalus) lipovitellins (Lvs) detected all three types of sea bass Vtg in the blood plasma of gravid females and/or estrogenized males and showed that all three forms of sea bass Lv undergo limited partial degradation during oocyte maturation. The comparatively high levels of VtgC-derived YPs in fully-grown oocytes and the maturational proteolysis of all three types of Lv differ from what has been reported for other teleosts spawning pelagic eggs in seawater but are similar to recent findings for two species of North American Moronidae, the striped bass (Morone saxatilis) and white perch (Morone americana), which spawn pelagic and demersal eggs, respectively in fresh water. Together with the high Vtg sequence homologies and virtually identical structural features of each type of Vtg between species, these findings indicate that the moronid multiple Vtg systems do not substantially vary with reproductive environment. PMID:26643259

  8. Interplay of posttranslational modifications in Sp1 mediates Sp1 stability during cell cycle progression.

    Science.gov (United States)

    Wang, Yi-Ting; Yang, Wen-Bin; Chang, Wen-Chang; Hung, Jan-Jong

    2011-11-18

    Although Sp1 is known to undergo posttranslational modifications such as phosphorylation, glycosylation, acetylation, sumoylation, and ubiquitination, little is known about the possible interplay between the different forms of Sp1 that may affect its overall levels. It is also unknown whether changes in the levels of Sp1 influence any biological cell processes. Here, we identified RNF4 as the ubiquitin E3 ligase of Sp1. From in vitro and in vivo experiments, we found that sumoylated Sp1 can recruit RNF4 as a ubiquitin E3 ligase that subjects sumoylated Sp1 to proteasomal degradation. Sp1 mapping revealed two ubiquitination-related domains: a small ubiquitin-like modifier in the N-terminus of Sp1(Lys16) and the C-terminus of Sp1 that directly interacts with RNF4. Interestingly, when Sp1 was phosphorylated at Thr739 by c-Jun NH(2)-terminal kinase 1 during mitosis, this phosphorylated form of Sp1 abolished the Sp1-RNF4 interaction. Our results show that, while sumoylated Sp1 subjects to proteasomal degradation, the phosphorylation that occurs during the cell cycle can protect Sp1 from degradation by repressing the Sp1-RNF4 interaction. Thus, we propose that the interplay between posttranslational modifications of Sp1 plays an important role in cell cycle progression and keeps Sp1 at a critical level for mitosis. PMID:21983342

  9. Pantoea vagans sp. nov., Pantoea eucalypti sp. nov., Pantoea deleyi sp. nov. and Pantoea anthophila sp. nov.

    Science.gov (United States)

    Brady, Carrie L; Venter, Stephanus N; Cleenwerck, Ilse; Engelbeen, Katrien; Vancanneyt, Marc; Swings, Jean; Coutinho, Teresa A

    2009-09-01

    Bacteria isolated from eucalyptus leaves and shoots showing symptoms of blight and die-back collected in Uganda, Uruguay and Argentina and from maize displaying brown stalk rot symptoms in South Africa were tentatively placed in the genus Pantoea on the basis of phenotypic and biochemical tests. These isolates, together with two strains (LMG 2558 and LMG 2560) previously assigned to Pantoea agglomerans based on protein electrophoregrams but later excluded from this species, were further investigated using molecular techniques. 16S rRNA gene sequencing and multilocus sequence analyses (MLSA) revealed that the strains were phylogenetically closely related to Pantoea agglomerans, Pantoea stewartii and Pantoea ananatis. MLSA and amplified fragment length polymorphism analysis placed the strains into four separate clusters, not containing any of the type strains of species of the genus Pantoea. DNA-DNA hybridization confirmed the classification of the isolates into four novel species, for which the names Pantoea vagans sp. nov. (type strain R-21566T=LMG 24199T=BCC 105T=BD 765T), Pantoea eucalypti sp. nov. (type strain R-25678T=LMG 24197T=BCC 076T=BD 769T), Pantoea deleyi sp. nov. (type strain R-31523T=LMG 24200T=BCC 109T=BD 767T) and Pantoea anthophila sp. nov. (type strain LMG 2558T=BD 871T=NCPPB 1682T) are proposed. PMID:19620357

  10. Productivity and river flux variability in response to the PETM on Atlantic margin at Bass River, NJ.

    Science.gov (United States)

    Stoll, H.; Shimizu, N.; Savain, R.; Zachos, J.; Ziveri, P.

    2009-04-01

    While the dramatic climate warming of the Paleocene-Eocene Thermal Maximum has been well characterized, changes in the hydrological cycle and the broader biogeochemical feedbacks (weathering, nutrients, productivity) are less well constrained. Here we describe new geochemical results from a coastal section on the midlatitude Atlantic margin of the U.S. at Bass River, NJ. We measured the elemental geochemistry of coccoliths to probe the productivity of these algae in response to the changing nutrient dynamics on the shelf in the time interval preceding and during the PETM. Coccoliths extracted from the siliclastic coastal section at Bass River NJ exhibit exceptionally good preservation and negligible overgrowth compared to typical ocean carbonate-rich sediments. Analysis of individual coccoliths using secondary ion mass spectrometry (SIMS) facilitates reliable trace element measurements in this low-carbonate section. Published sequence stratigraphy and microfossil analysis have revealed several third order sea level cycles in the late Paleocene including a highstand during the PETM. Consequently we extend our paleoproductivity records far below the PETM to characterize this background variability. We recognize a pattern of generally maximum productivity during lowstands and minimal productivity during highstands. Because nutrient concentrations decrease significantly with distance from the coast, highstands reduce productivity by shifting the highest nutrient levels landward, away from the site. This is likely due to greater distance from river sources as well as reduced wave turbulence which mixes nutrients into the photic zone. This general pattern is broken during the PETM, which features high productivity despite a sea level highstand. This anomalous high productivity may reflect enhanced riverine nutrient delivery, and potentially changes in wind strength and mixing intensity. Riverine nutrient delivery could increase with higher precipitation or precipitation

  11. A novel method to develop an otolith microchemistry model to determine striped bass habitat use in the San Francisco Estuary

    Energy Technology Data Exchange (ETDEWEB)

    Phillis, C C; Ostrach, D J; Gras, M; Yin, Q; Ingram, B L; Zinkl, J G; Weber, P K

    2006-06-14

    Otolith Sr/Ca has become a popular tool for hind casting habitat utilization and migration histories of euryhaline fish. It can readily identify habitat shifts of diadromous fish in most systems. Inferring movements of fish within estuarine habitat, however, requires a model of that accounts of the local water chemistry and the response of individual species to that water chemistry, which is poorly understood. Modeling is further complicated by the fact that high marine Sr and Ca concentrations results in a rapid, nonlinear increase in water Sr/Ca and {sup 87}Sr/{sup 86}Sr between fresh and marine waters. Here we demonstrate a novel method for developing a salinity-otolith Sr/Ca model for the purpose of reconstructing striped bass (Morone saxatilis) habitat use in the San Francisco Bay estuary. We used correlated Sr/Ca and {sup 87}Sr/{sup 86}Sr ratios measurements from adult otoliths from striped bass that experienced a range of salinities to infer striped bass otolith Sr/Ca response to changes in salinity and water Sr/Ca ratio. Otolith {sup 87}Sr/{sup 86}Sr can be assumed to accurately record water {sup 87}Sr/{sup 86}Sr because there is no biological fractionation of Sr isotopes. Water {sup 87}Sr/{sup 86}Sr can in turn be used to estimate water salinity based on the mixing of fresh and marine water with known {sup 87}Sr/{sup 86}Sr ratios. The relationship between adjacent analyses on otoliths of Sr/Ca and {sup 87}Sr/{sup 86}Sr by LA-ICP-MS and MC-ICP-MS (r{sup 2} = 0.65, n = 66) is used to predict water salinity from a measured Sr/Ca ratio. The nature of this non-linear model lends itself well to identifying residence in the Delta and to a lesser extent Suisun Bay, but does not do well locating residence within the more saline bays west of Carquinez Strait. An increase in the number of analyses would improve model confidence, but ultimately the precision of the model is limited by the variability in the response of individual fish to water Sr/Ca.

  12. An Sp1/Sp3 binding polymorphism confers methylation protection.

    Directory of Open Access Journals (Sweden)

    Yanis A Boumber

    Full Text Available Hundreds of genes show aberrant DNA hypermethylation in cancer, yet little is known about the causes of this hypermethylation. We identified RIL as a frequent methylation target in cancer. In search for factors that influence RIL hypermethylation, we found a 12-bp polymorphic sequence around its transcription start site that creates a long allele. Pyrosequencing of homozygous tumors revealed a 2.1-fold higher methylation for the short alleles (P<0.001. Bisulfite sequencing of cancers heterozygous for RIL showed that the short alleles are 3.1-fold more methylated than the long (P<0.001. The comparison of expression levels between unmethylated long and short EBV-transformed cell lines showed no difference in expression in vivo. Electrophorectic mobility shift assay showed that the inserted region of the long allele binds Sp1 and Sp3 transcription factors, a binding that is absent in the short allele. Transient transfection of RIL allele-specific transgenes showed no effects of the additional Sp1 site on transcription early on. However, stable transfection of methylation-seeded constructs showed gradually decreasing transcription levels from the short allele with eventual spreading of de novo methylation. In contrast, the long allele showed stable levels of expression over time as measured by luciferase and approximately 2-3-fold lower levels of methylation by bisulfite sequencing (P<0.001, suggesting that the polymorphic Sp1 site protects against time-dependent silencing. Our finding demonstrates that, in some genes, hypermethylation in cancer is dictated by protein-DNA interactions at the promoters and provides a novel mechanism by which genetic polymorphisms can influence an epigenetic state.

  13. Reproductive and Endocrine Biomarkers in Largemouth Bass (Micropterus salmoides) and Common Carp (Cyprinus carpio) from United States Waters

    Science.gov (United States)

    Goodbred, Steven L.; Smith, Stephen B.; Greene, Patricia S.; Rauschenberger, Richard H.; Bartish, Timothy M.

    2007-01-01

    A nationwide reconnaissance investigation was initiated in 1994 to develop and evaluate a suite of reproductive and endocrine biomarkers for their potential to assess reproductive health and status in teleost (bony) fish. Fish collections were made at 119 sites, representing many regions of the country and land- and water-use settings. Collectively, this report will provide a national and regional benchmark and a basis for evaluating biomarkers of endocrine and reproductive function. Approximately 2,200 common carp (Cyprinus carpio) and 650 largemouth bass (Micropterus salmoides) were collected from 1994 through 1997. The suite of biomarkers used for these studies included: the plasma sex-steroid hormones, 17?-estradiol (E2) and 11 ketotestosterone (11KT); the ratio of E2 to 11KT (E2:11KT); plasma vitellogenin (VTG); and stage of gonadal development. This data report provides fish size, stage and reproductive biomarker data for individual fish and for site and regional summaries of these variables.

  14. Protection des ions organiques contre les dommages induits a l'ADN par les electrons de basse energie

    Science.gov (United States)

    Dumont, Ariane

    Il a ete demontre que les electrons de basse energie (EBE) peuvent induire des cassures simple brin (CSB) a l'ADN, via la formation d'anions transitoires qui decroissent par attachement dissociatif, ou dans d'autres etats electroniques dissociatifs menant a la fragmentation. Afin d'effectuer une etude complete des effets des electrons de basse energie sur la matiere biologique, il est necessaire de comprendre leur mecanismes d'interaction non seulement avec l'ADN, mais avec les constituants de son environnement. Les histones sont une composante importante de l'environnement moleculaire de l'ADN. Leur charge positive leur permet de s'associer aux groupements phosphate anionique de l'ADN. Le role principal de ces proteines basiques consiste a organiser l'ADN et l'empaqueter afin de former la chromatine. Les cations sont une autre composante importante de la cellule; ils jouent un role dans la stabilisation de la conformation B de l'ADN in vitro par leurs interactions avec les petits et grands sillons de l'ADN, ainsi qu'avec le groupement phosphate charge negativement. Avec les histones, ils participent egalement a la compaction de l'ADN pour former la chromatine. Cette etude a pour but de comprendre comment la presence d'ions organiques (sous forme de Tris et d'EDTA) a proximite de l'ADN modifie le rendement de cassures simple brin induit par les electrons de basse energie. Le Tris et l'EDTA ont-ete choisis comme objet d'etude, puisqu'en solution, ils forment le tampon standard pour solubiliser l'ADN dans les experiences in vitro (10mM Tris, 1mM EDTA). De plus, la molecule Tris possede un groupement amine alors que l'EDTA possede 4 groupements carboxyliques. Ensembles, ils peuvent se comporter comme un modele simple pour les acides amines. Le ratio molaire de 10 :1 de Tris par rapport a l'EDTA a pour but d'imiter le comportement des histones qui sont riches en arginine et lysine, acides amines possedant un groupement amine charge positivement additionnel. Des films d

  15. Deux extraits commentés des Basses Œuvres1 de ‘Abduh Khāl

    OpenAIRE

    Lagrange, Frédéric

    2014-01-01

    Le romancier saoudien ‘Abduh Khāl, dans son roman Tarmī bi‑sharar (Les Basses Œuvres) paru en 2009 et couronné par l’International Prize for Arab Fiction en 2010, tend un miroir peu flatteur au Royaume wahhabite : ce monologue d’un tortionnaire violeur au service d’un mystérieux Maître du Palais dans une Djedda déformée par l’affairisme figurant allégoriquement le régime présente un pays corrompu par sa soumission à l’absolutisme. Deux extraits sont ici commentés, en soulignant les rapports i...

  16. The applicability of Bass's model of transformational, transactional, and laissez-faire leadership in the hospital administrative environment.

    Science.gov (United States)

    Spinelli, Robert J

    2006-01-01

    The purpose of this study is to evaluate empirically in the hospital administrative environment the relationship of leadership behaviors to subordinate manager's perceived outcomes, through examination of B. M. Bass's (1985) model of transformational, transactional, and laissez-faire leadership. The author measured leadership orientation and outcome factors through subordinate managers' ratings of hospital CEOs using a questionnaire, which asked: Is there a relationship between the leadership styles of hospital CEOs and subordinate managers' self-reported willingness to exert extra effort, perception of leader effectiveness and satisfaction with their leader? Findings revealed that the relationship between transformational leadership and the outcome factors were stronger and more positive than were the transactional and laissez-faire styles. These findings are consistent with the hierarchal patterns reported and support the universality of the model. PMID:16708688

  17. Les nouveaux colzas à basse teneur en glucosilonates : composition chimique et valeur nutritive des tourteaux et graines pour le porc

    OpenAIRE

    Bourdon, D.

    1989-01-01

    Les données de valeur nutritive (valeurs énergétique et azotée) de 30 échantillons de tourteaux et graines de colza à haute (HG) et basse (BG) teneur en glucosinolates, obtenues par mesure de digestibilité directe sur porc à l’engrais, sont présentées par type de tourteau (extrait au solvant, de pression, normal et dépelliculé), de même que pour les graines traitées. Nos résultats expérimentaux sont comparés à un nombre équivalent de données bibliographiques disponibles. Ils permettent ...

  18. Etude de nano-transistors à faible pente sous le seuil pour des applications très basse consommation

    OpenAIRE

    Villalon, Anthony

    2014-01-01

    Le transistor à effet tunnel bande à bande (TFET) est une architecture PIN à grille capable d’obtenir une pente sous le seuil inférieure à 60mV/dec à température ambiante, ce qui représente un avantage par rapport au MOSFET dans le cas d’applications basse consommation. L’objectif de cette thèse est d’étudier et de caractériser des TFETs fabriqués au CEA-LETI (sur substrats SOI avec les procédés standards CMOS), afin de comprendre et d’optimiser ces dispositifs. La première génération de TFET...

  19. Effect of dietary fat level on carcass traits and flesh quality of European Sea Bass (Dicentrarchus labrax from mariculture

    Directory of Open Access Journals (Sweden)

    Severino Segato

    2010-01-01

    Full Text Available The study aimed at evaluating the effect of the reduction of dietary fat on juvenile European sea bass nutritional value and quality traits. Fish were reared in floating cages (Trieste Gulf, Italy from July (11 to October (10. Two isoproteic diets were compared: LF (low fat, EE = 19.4% vs. HF (high fat, EE = 24.6%. No significantly different growth performance was observed. LF diet-fed fish were characterized by the reduction of celomatic fat (not edible fraction and by the increase in dressing percentage. The tested dietary fat level also affected both fillet and epiaxial white muscle proximate composition, resulting in a significantly lower fillet lipid concentration in LF diet-fed fish. Dietary treatment influenced cooked fillet colour and texture probably as a consequence of the different intramuscular fat deposition. Fillet from HF-fed fish, in fact, presented higher lightness (L* value and lower instrumental strengthness.

  20. Electron beam irradiation, oxygen, and temperature effects on nucleotide degradation in stored aquaculture hybrid striped bass fillets

    International Nuclear Information System (INIS)

    Skinless fillets from commercially-grown aquaculture hybrid striped bass (Morone saxatilis x M. chrysops) were electron beam-irradiated in the presence of air or vacuum-packaged and stored at 4C and -20C for 14 days. A mean low dose level of 2.0 or 3.0 kGy (+/- 0.5 kGy) and high dose level of 20 kGy (+/- 4 kGy) were used for irradiated samples. Hypoxanthine (Hx) concentrations, Ki-values ([(INO + Hx)/(IMP + INO + Hx)] x 100), and H-values ([(Hx)/(IMP + INO + Hx)] x 100) indicated that irradiation did not influence the rate of nucleotide degradation compared with nonirradiated controls at either refrigerated or frozen temperatures. Vacuum packaging or freezing of stored samples resulted in lower H-values and Hx contents compared with nonirradiated controls regardless of irradiation treatment

  1. Ambient and potential denitrification rates in marsh soils of Northeast Creek and Bass Harbor Marsh watersheds, Mount Desert Island, Maine

    Science.gov (United States)

    Huntington, Thomas G.; Culbertson, Charles W.; Duff, John H.

    2012-01-01

    Nutrient enrichment from atmospheric deposition, agricultural activities, wildlife, and domestic sources is a concern at Acadia National Park on Mount Desert Island, Maine, because of the potential problems of degradation of water quality and eutrophication in estuaries. Degradation of water quality has been observed at Bass Harbor Marsh estuary in the park but only minimally in Northeast Creek estuary. Previous studies at Acadia National Park have estimated nutrient inputs to estuaries from atmospheric deposition and surface-water runoff, and have identified shallow groundwater as an additional potential source of nutrients. Previous studies at Acadia National Park have assumed that a certain fraction of the nitrogen input was removed through microbial denitrification, but rates of denitrification (natural or maximum potential) in marsh soils have not been determined. The U.S. Geological Survey, in cooperation with Acadia National Park, measured in-place denitrification rates in marsh soils in Northeast Creek and in Bass Harbor Marsh watersheds during summer 2008 and summer 2009. Denitrification was measured under ambient conditions as well as after additions of inorganic nitrogen and glucose. In-place denitrification rates under ambient conditions were similar to those reported for other coastal wetlands, although they were generally lower than those reported for salt marshes having high ambient concentrations of nitrate (NO3). Denitrification rates generally increased by at least an order of magnitude following NO3 additions, with or without glucose (as the carbohydrate) additions, compared with the ambient treatments that received no nutrient additions. The treatment that added both glucose and NO3 resulted in a variety of denitrification responses when compared with the addition of NO3 alone. In most cases, the addition of glucose to a given rate of NO3 addition resulted in higher rates of denitrification. These variable responses indicate that the amount of

  2. Effects of starvation on protein synthesis and nucleic acid metabolism in the muscle of the barred sand bass Paralabrax nebulifer

    International Nuclear Information System (INIS)

    Starvation induced different protein synthesis responses in red and white muscle of the barred sand bass Paralabrax nebulifer. Red muscle had 14C-leucine incorporation rates into total protein which were several times higher than white muscle in both the fed and starved states. Muscle was separated into a myofibrillar fraction consisting of the structural proteins and a sarcoplasmic fraction consisting of soluble proteins. Synthesis of the myofibrillar fraction of white muscle decreased by 90%, while red muscle myofibrillar synthesis remained essentially unchanged. Changes in the labeling of several enzymes purified from the sarcoplasmic fraction were different even though the overall loss of enzyme activity was similar, suggesting that changes in synthesis rates were important in maintaining appropriate relative enzyme concentrations

  3. Dworshak Dam Impact Assessment and Fishery Investigation and Trout, Bass and Forage Species: Combined Project Completion Report.

    Energy Technology Data Exchange (ETDEWEB)

    Maiolie, Melo; Statler, David P.; Elam, Steve

    1992-10-01

    The Nez Perce Tribe (NPT) and the Idaho Department of Fish and Game (IDFG) entered into separate intergovernmental agreements with the Bonneville Power Administration in a cooperative four-year effort to study impacts of Dworshak Dam operation on resident fisheries. The NPT Department of Fisheries Management focused on rainbow trout, smallmouth bass and forage fish. The IDFG's segment of the project was to document kokanee population dynamics, relate it to the changing nutrient status of the reservoir, evaluate kokanee losses through Dworshak Dam, and make kokanee management recommendations. This final report includes findings for 1990 and 1991 and relates these data to information previously presented in annual reports for 1987, 1988 and 1989.

  4. Does Virulence Assessment of Vibrio anguillarum Using Sea Bass (Dicentrarchus labrax) Larvae Correspond with Genotypic and Phenotypic Characterization?

    DEFF Research Database (Denmark)

    Frans, Ingeborg; Dierckens, Kristof; Crauwels, Sam;

    2013-01-01

    Background: Vibriosis is one of the most ubiquitous fish diseases caused by bacteria belonging to the genus Vibrio such as Vibrio (Listonella) anguillarum. Despite a lot of research efforts, the virulence factors and mechanism of V. anguillarum are still insufficiently known, in part because of the......'s Phenotype MicroArray (TM) technology and some virulence factor assays. Conclusions/Significance: Virulence testing revealed ten virulent and five avirulent strains. While some relation could be established between serotype, genotype and phenotype, no relation was found between virulence and genotypic or...... lack of standardized virulence assays. Methodology/Principal Findings: We investigated and compared the virulence of 15 V. anguillarum strains obtained from different hosts or non-host niches using a standardized gnotobiotic bioassay with European sea bass (Dicentrarchus labrax L.) larvae as model...

  5. Evaluation of different methods of stunning/killing sea bass (Dicentrarchus labrax) by tissue stress/quality indicators.

    Science.gov (United States)

    Zampacavallo, Giulia; Parisi, Giuliana; Mecatti, Massimo; Lupi, Paola; Giorgi, Gianluca; Poli, Bianca Maria

    2015-05-01

    The aim of the study was to evaluate the effect on the final product quality of certain innovative stunning/killing methods for sea bass as substitutes for the most common methods used by European farmers. The changes in tissue stress/quality parameters were monitored from the first hours after death and during the shelf life of the fish. Two trials were conducted in July and November on n. 231 sea bass stunned/killed by ice-water slurry, by single gas or mixture of gases in ice-water and by single- or two-stage electrical stunning/killing methods. Behavioural responses, stun/death time, rigor index, muscular and ocular pH, lactic acid, ATP and catabolites at death and within the 24 h after death were determined. In the November trial, the sensorial evaluation, rigor index, IMP, inosine, hypoxanthine, and K1 values were also evaluated during refrigerated storage until spoilage. The stunning/killing in ice-water appeared to induce low effects on the analysed parameters and preserve a good product quality as indicated by the highest pH and ATP values at death, the delayed full rigor onset and the 1 day longer shelf life (14 days) in comparison with the single- or two-stage electrical stunning/killing. The gas mixture addition provided a 40 % shortening of the time to obtain stunning/killing and 14 days of shelf life. The actual level of quality loss with the different killing conditions and the actual impact of a significant shortage of rigor mortis onset and pH drop on the possible pre-rigor filleting remain to be studied in depth. PMID:25892757

  6. Photoperiodic Modulation of Circadian Clock and Reproductive Axis Gene Expression in the Pre-Pubertal European Sea Bass Brain

    Science.gov (United States)

    Martins, Rute S. T.; Gomez, Ana; Zanuy, Silvia; Carrillo, Manuel; Canário, Adelino V. M.

    2015-01-01

    The acquisition of reproductive competence requires the activation of the brain-pituitary-gonad (BPG) axis, which in most vertebrates, including fishes, is initiated by changes in photoperiod. In the European sea bass long-term exposure to continuous light (LL) alters the rhythm of reproductive hormones, delays spermatogenesis and reduces the incidence of precocious males. In contrast, an early shift from long to short photoperiod (AP) accelerates spermatogenesis. However, how photoperiod affects key genes in the brain to trigger the onset of puberty is still largely unknown. Here, we investigated if the integration of the light stimulus by clock proteins is sufficient to activate key genes that trigger the BPG axis in the European sea bass. We found that the clock genes clock, npas2, bmal1 and the BPG genes gnrh, kiss and kissr share conserved transcription factor frameworks in their promoters, suggesting co-regulation. Other gene promoters of the BGP axis were also predicted to be co-regulated by the same frameworks. Co-regulation was confirmed through gene expression analysis of brains from males exposed to LL or AP photoperiod compared to natural conditions: LL fish had suppressed gnrh1, kiss2, galr1b and esr1, while AP fish had stimulated npas2, gnrh1, gnrh2, kiss2, kiss1rb and galr1b compared to NP. It is concluded that fish exposed to different photoperiods present significant expression differences in some clock and reproductive axis related genes well before the first detectable endocrine and morphological responses of the BPG axis. PMID:26641263

  7. Cloning, tissue distribution and effects of fasting on pituitary adenylate cyclase-activating polypeptide in largemouth bass

    Science.gov (United States)

    Li, Shengjie; Han, Linqiang; Bai, Junjie; Ma, Dongmei; Quan, Yingchun; Fan, Jiajia; Jiang, Peng; Yu, Lingyun

    2015-03-01

    Pituitary adenylate cyclase activating polypeptide (PACAP) has a wide range of biological functions. We cloned the full-length cDNAs encoding PACAP and PACAP-related peptide (PRP) from the brain of largemouth bass ( Micropterus salmoides) and used real-time quantitative PCR to detect PRP-PACAP mRNA expression. The PRP-PACAP cDNA has two variants expressed via alternative splicing: a long form, which encodes both PRP and PACAP, and a short form, which encodes only PACAP. Sequence analysis results are consistent with a higher conservation of PACAP than PRP peptide sequences. The expression of PACAP-long and PACAP-short transcripts was highest in the forebrain, followed by the medulla, midbrain, pituitary, stomach, cerebellum, intestine, and kidney; however, these transcripts were either absent or were weakly expressed in the muscle, spleen, gill, heart, fatty tissue, and liver. The level of PACAP-short transcript expression was significantly higher than expression of the long transcript in the forebrain, cerebella, pituitary and intestine, but lower than that of the long transcript in the stomach. PACAP-long and PACAP-short transcripts were first detected at the blastula stage of embryogenesis, and the level of expression increased markedly between the muscular contraction stage and 3 d post hatch (dph). The expression of PACAP-long and PACAP-short transcripts decreased significantly in the brain following 4 d fasting compared with the control diet group. The down-regulation effect was enhanced as fasting continued. Conversely, expression levels increased significantly after 3 d of re-feeding. Our results suggest that PRP-PACAP acts as an important factor in appetite regulation in largemouth bass.

  8. VACCINATION OF HYBRID STRIPED BASS: GROWTH, IMMUNE REACTION AND GENE EXPRESSION

    Directory of Open Access Journals (Sweden)

    Mark Westerman

    2012-10-01

    Full Text Available Hybrid striped bass (42.6+4.9 g wet wt; 139.3+6.1 mm length, were randomly stocked into one of 6 tanks (n=6 tank-1 of a custom designed, recirculating life support system (RLSS. Water quality was as follows: DO2 (6.50.6 mg l-1, pH (7.70.5, TAN (0.06-1.31 mg l-1, nitrite (0.06-0.60 mg l-1 and nitrate (2.0-32.1 mg l-1, salinity 5 ppt, temperature 28±1 oC. A 12:12 photophase:scotophase was used, with a 30 min. dusk-dawn dimming of lights. Fish were fed at 4% body wt d-1 as two separate feedings (08.00 and 16.00 h. Dietary crude protein and lipid levels were 40% and 10% respectively. Tanks were randomly paired and fish either left untreated, vaccinated, or sham injected. The vaccine employed was an experimental formalin killed Streptococcus iniae oil-in-water adjuvanted bacterin. Fish were weighed and measured bimonthly for 8 wk with group weights being employed to adjust feeding rates. At trial termination, all animals were weighed and measured, and their condition factor (CF and feed conversion ratios (FCR calculated. Visceral somatic (VSI and hepatosomatic (HSI indices and intraperitoneal fat (IPF and muscle ratios (MR were also assessed. Blood was taken from anaesthetized fish and hematocrit recorded. Blood was allowed to clot overnight at 5 oC after which serum protein levels were recorded and lysozyme activity measured. Livers were prepared for microarray evaluation using standard techniques using a Danio rerio genechip to assess global changes in gene expression. No differences were observed with respect to final weights, lengths, CF, FCR, or HSI although differences (P < 0.001 were determined for VSI, which was higher in control animals. Packed cell volume and serum protein levels were similar across groups (P > 0.05. Time-course of changes in serum lysozyme activity described an initial reduction in lysozyme activity followed by a rebounding in activity which peaked 25 days post-treatments. Evaluation of lysozyme activity among time

  9. SP-100 Reactor Subsystem Development

    Science.gov (United States)

    Demuth, Scott F.

    1994-07-01

    The SP-100 reactor subsystem consists of the pressure vessel, vessel internals, and fuel elements. Type A (standard) Nb-1Zr and rhenium materials development efforts related to fabrication of the vessel, vessel internals, and fuel cladding/liner have been completed. Type A and Type C (PWC-11) Nb-1Zr loop fabrication has been successfully demonstrated by prototypic testing with flowing lithium at 1350 K for 1500 hr. Development of UN fuel has been completed, and the performance validated by irradiation testing to the full life (7 yr. full power) burnup of 6 atom %. Neutronic and hydraulic core performance have been validated by engineering mockup critical experiments in the Zero Power Physics Reactor at Argonne National Laboratory, and detailed core hydraulic flow testing with water. Essentially all feasibility issues have been settled for the full life SP-100 reactor subsystem. Remaining SP-100 reactor subsystem development efforts are focused on further reducing mass by the use of Type C (PWC-11) Nb-1Zr rather than Type A, and demonstrating fuel life for beyond full life to perhaps 9 atom % burnup.

  10. Metabolism of the polycyclic musk galaxolide and its interference with endogenous and xenobiotic metabolizing enzymes in the European sea bass (Dicentrarchus labrax).

    Science.gov (United States)

    Fernandes, Denise; Dimastrogiovanni, Giorgio; Blázquez, Mercedes; Porte, Cinta

    2013-03-01

    This study investigates the metabolism and mode of action of galaxolide (HHCB) in the European sea bass -Dicentrarchus labrax- following a single intraperitoneal injection of 50 mg HHCB/kg body weight. In addition, a group of fish was injected with 50 mg/kg of ketoconazole (KCZ), a fungicide that is known to interfere with different Cyp isoenzymes. HHCB was actively metabolised by sea bass and acted as a weak inhibitor of the synthesis of oxyandrogens in gonads of male fish. Both, HHCB and a hydroxylated metabolite were detected in bile. The fungicide ketoconazole was a strong inhibitor of Cyp11β and Cyp3a-catalyzed activities. The work contributes to the better understanding of the impact of synthetic musks on fish and proposes the determination of HHCB and/or its hydroxylated metabolite in bile as a tool to assess environmental exposure in wild fish. PMID:23274450

  11. Energy savings in sea bass swimming in a school: measurements of tail beat frequency and oxygen consumption at different swimming speeds

    DEFF Research Database (Denmark)

    Herskin, J; Steffensen, JF

    1998-01-01

    Tail beat frequency of sea bass, Dicentrarchus labrax (L.) (23.5 ± 0·5 cm, LT), swimming at the front of a school was significantly higher than when swimming at the rear, for all water velocities tested from 14·8 to 32 cm s-1. The logarithm of oxygen consumption rate, and the tail beat frequency of...... solitary swimming sea bass (28·8 ± 0·4 cm, LT), were each correlated linearly with swimming speed, and also with one another. The tail beat frequency of individual fish was 9-14% lower when at the rear of a school than when at the front, corresponding to a 9-23% reduction in oxygen consumption rate....

  12. Comparison of zootechnical performances, welfare condition and quality traits of hybrid striped bass (Morone chrysops x Morone saxatilis reared in different Italian farms

    Directory of Open Access Journals (Sweden)

    Paolo Melotti

    2010-01-01

    Full Text Available Growth performances of sunshine bass (initial m.b.w.=200±50 g were examined in 3 different rearing conditions: concrete square basins (HSB-1; raceways (HSB-2; natural ponds (HSB-3. Fish received the same extruded feed (prot. 48.8%; lip. 17.8%. The trial lasted 16 months in HSB-1 and HSB-2 and only 12 months in HSB-3 due to unfavourable welfare status and low growth performances. At harvest (after 16 months, sunshine bass reached a satisfactory mean weight with- out significant differences between groups. HSB-2 fish were longer with a smaller circumference, prob- ably due to different hydrodynamics of the raceway. Glucose, cholesterol, triglycerides, protein, lactic dehydrogenase (LDH, aspartate aminotransferase (AST, alanine aminotransferase (ALT, alkaline phosphatase (ALP, creatine kinase (CK, glycogen of plasma or tissue were not affected by rearing conditions as well as the proximate composition and the fatty acid profile of the fillet.

  13. A new species of dwarf sea bass, genus Serranus (Serranidae: Actinopterygii, from the southwestern Atlantic Ocean

    Directory of Open Access Journals (Sweden)

    Alfredo Carvalho Filho

    2013-01-01

    Full Text Available Serranus aliceae n. sp. is described from Rio de Janeiro and Espírito Santo States, southeastern Brazilian coast. The species is readily distinguished from its congeners by the following combination of characters: scales around the caudal peduncle 20 or 21; total gill rakers on first branchial arch 23-29; cheek-scales rows 5 or 6; and by the coloration of live specimens, which are reddish with a broad, conspicuous, white stripe from post-orbital region to the caudal-fin base and a white abdomen. The new species apparently inhabits the deeper (30 or more meters portions of reef environments along the subtropical Brazilian coast, and is possibly associated with upwelling cold-water masses. The species was observed hovering above rocky bottoms, feeding on micro-crustaceans, squid larvae, and zooplankton. This new species increases to eight the number of valid Serranus species recorded in the southwestern Atlantic.

  14. Organochlorine pesticides and thiamine in eggs of largemouth bass and American alligators and their relationship with early life-stage mortality

    Science.gov (United States)

    Sepulveda, M.S.; Wiebe, J.J.; Honeyfield, D.C.; Rauschenberger, H.R.; Hinterkopf, J.P.; Johnson, W.E.; Gross, T.S.

    2004-01-01

    Thiamine deficiency has been linked to early mortality syndrome in salmonids in the Great Lakes. This study was conducted to compare thiamine concentrations in American alligators (Alligator mississippiensis) and Florida largemouth bass (Micropterus salmoides floridanus) eggs from sites with high embryo mortality and high exposure to organochlorine pesticides (OCPs) (Lakes Apopka and Griffin, and Emeralda Marsh, Florida, USA) to those from sites that have historically exhibited low embryo mortality and low OCPs (Lakes Woodruff and Orange, Florida). During June-July 2000, 20 alligator clutches were collected from these sites, artificially incubated, and monitored for embryo mortality. Thiamine and OCPs were measured in one egg/clutch. During February 2002, 10 adult female bass were collected from Emeralda Marsh and Lake Woodruff and mature ovaries analyzed for thiamine and OCP concentrations. Although ovaries from the Emeralda Marsh bass contained almost 1,000-fold more OCPs compared with the reference site, Lake Woodruff, there were no differences in thiamine concentrations between sites (11,710 vs. 11,857 pmol/g). In contrast, alligator eggs from the reference site had five times the amount of thiamine compared with the contaminated sites (3,123 vs. 617 pmol/g). Similarly, clutches with > 55% hatch rates had significantly higher concentrations of thiamine compared with clutches with alligator embryo survival but not in reproductive failure and recruitment of largemouth bass. The cause(s) of this thiamine deficiency are unknown but might be related to differences in the nutritional value of prey items across the sites studied and/or to the presence of high concentration of contaminants in eggs. ?? Wildlife Disease Association 2004.

  15. Antioxidant, antimicrobial and sensorial effects of Rosemary (Rosmarinus officinalis L.) and Sage (Salvia officinalis L.) on Sea bass (Dicentrarchus labrax L.) fillets

    OpenAIRE

    Can Altınelataman; Duygu Kışla; Berna Kılınç; Evren Burcu Şen Yılmaz; Aslı Cadun Yünlü; Tolga Dinçer; Ufuk Çelik

    2015-01-01

    In this study, effects of sum of vapor distillate and water phase extract of rosemary and sage leaves on sea bass fillets quality, were investigated. For this purpose, effective solution was obtained with same amounts of these plants, cooled down to room temperature, applied to fillets and samples were stored in refrigerated conditions during 13 days. In monitoring period, pH, TVB-N (Total Viable Base –Nitrogen), TBA (Tiobarbutiric acid ), color, textural, microbiological and sensorial analys...

  16. Effects of persistent organic pollutants on the thyroid function of the European sea bass (Dicentrarchus labrax) from the Aegean Sea, is it an endocrine disruption?

    OpenAIRE

    Schnitzler, Joseph; Koutrarkis, Emmanuil; Siebert, Ursula; Thomé, Jean-Pierre; Das, Krishna

    2008-01-01

    We evaluated the alterations of organochlorinated compounds such as polychlorobiphenyls (PCB), dichloro-diphenyl-dichloroethylene (DDE) and dichloro-diphenyl-trichloroethane (DDT) on the thyroid in wild and cultured sea bass (Dicentrarchus labrax) at environmental concentrations. These compounds influence the endocrine system of many fish species and are qualified as endocrine disruptors. The thyroid seems to be a target organ. Two alteration endpoints: the thyroid histology and the muscular ...

  17. One loop effects to the process e+e-→b-bass in topcolor-assisted multi-scale technicolor model

    International Nuclear Information System (INIS)

    The authors calculated the Pseudo-Goldstone bosons (technipions, top-pions) corrections to the cross-section σ of the process e+e-→b-bass in topcolor-assisted multi-scale technicolor model at LEP II energy region. The results show that, with reasonable value of the parameters, the cross-section is one order larger than that predicted by the standard model. The authors discussed the possibility of detecting this rare event at the 'B-factory'

  18. Improving oral hygiene skills by computer-based training : A randomized controlled comparison of the modified bass and the fones techniques

    OpenAIRE

    Harnacke, Daniela; Mitter, Simona; Lehner, Marc; Munzert, Jörn; Deinzer, Renate

    2012-01-01

    Background: Gingivitis and other plaque-associated diseases have a high prevalence in western communities even though the majority of adults report daily oral hygiene. This indicates a lack of oral hygiene skills. Currently, there is no clear evidence as to which brushing technique would bring about the best oral hygiene skills. While the modified Bass technique is often recommended by dentists and in textbooks, the Fones technique is often recommended in patient brochures. Still, standardize...

  19. The potential use of legume-based diets supplemented with microbial phytase on the growth performance and feed efficiency of sea bass, Lates calcarifer

    Directory of Open Access Journals (Sweden)

    Erlinda S. Ganzon-Naret

    2013-09-01

    Full Text Available A feeding trial was conducted for 12 weeks to evaluate the potential use of legume-based diets supplemented with dietary microbial phytase on the growth performance and feed efficiency of juvenile sea bass, Lates calcarifer. Fifteen sea bass juveniles (mean initial weight of 0.96 g and mean initial total length (TL of 4.2 cm were stocked at three replicates into each of the twelve 100 L conical fibreglass tanks containing 90 L sea water in a closed recirculating system with filtered and aerated sea water. Four isonitrogenous, isolipidic and isocaloric experimental diets were formulated. The control diet (C0 contained fish meal, soybean meal, shrimp meal and squid meal as major protein sources. Legume seed meals of pigeon pea (Cajanus cajan, yellow mungbeans (Phaseolus aureus, and green mungbeans (Vigna radiata were incorporated in the practical diets D1, D2 and D3 respectively at 18-20% replacing an equivalent amount of 6-7g fish meal protein and supplemented with microbial phytase at the level of 300U kg-1 diet. Growth rate, feed conversion ratio (FCR, protein efficiency ratio (PER and apparent net protein utilization (ANPU of sea bass were significantly (P < 0.05 higher in control diet than those given different legume based diets supplemented with phytase. Histological examination of the liver tissues for the different dietary treatments did not manifest any abnormalities. Phytase supplementation also improved bone ash, phosphorus (P concentration as well as P content in the carcass for fish in legume fed groups. Results from the present study showed that incorporation of dietary microbial phytase in legume based diets slightly improve the growth performance and P availability in sea bass juveniles.

  20. The potential use of legume-based diets supplemented with microbial phytase on the growth performance and feed efficiency of sea bass, Lates calcarifer

    Directory of Open Access Journals (Sweden)

    Erlinda S. Ganzon-Naret

    2013-09-01

    Full Text Available A feeding trial was conducted for 12 weeks to evaluate the potential use of legume-based diets supplemented with dietary microbial phytase on the growth performance and feed efficiency of juvenile sea bass, Lates calcarifer. Fifteen sea bass juveniles (mean initial weight of 0.96 g and mean initial total length (TL of 4.2 cm were stocked at three replicates into each of the twelve 100 L conical fibreglass tanks containing 90 L sea water in a closed recirculating system with filtered and aerated sea water. Four isonitrogenous, isolipidic and isocaloric experimental diets were formulated. The control diet (C0 contained fish meal, soybean meal, shrimp meal and squid meal as major protein sources. Legume seed meals of pigeon pea (Cajanus cajan, yellow mungbeans ((Phaseolus aureus,and green mungbeans ((Vigna radiata were incorporated in the practical diets D1, D2 and D3 respectively at 18-20% replacing an equivalent amount of 6-7g fish meal protein and supplemented with microbial phytase at the level of 300U kg-1 diet. Growth rate, feed conversion ratio (FCR, protein efficiency ratio (PER and apparent net protein utilization (ANPU of sea bass were significantly (P < 0.05 higher in control diet than those given different legume based diets supplemented with phytase. Histological examination of the liver tissues for the different dietary treatments did not manifest any abnormalities. Phytase supplementation also improved bone ash, phosphorus (P concentration as well as P content in the carcass for fish in legume fed groups. Results from the present study showed that incorporation of dietary microbial phytase in legume based diets slightly improve the growth performance and P availability in sea bass juveniles.

  1. Rates of consumption of juvenile salmonids and alternative prey fish by northern squawfish, walleyes, smallmouth bass, and channel catfish in John Day Reservoir, Columbia River

    International Nuclear Information System (INIS)

    Adult northern squawfish Ptychocheilus oregonesis, walleyes Stizostedion vitreum, smallmouth bass Micropterus dolomieu, and channel catfish Ictalurus punctatus were sampled from four regions of John Day Reservoir from April to August 1983-1986 to quantify their consumption of 13 species of prey fish, particularly seaward-migrating juvenile Pacific salmon and steelhead (Oncorhynchus spp.). Consumption rates were estimated from field data on stomach contents and digestion rate relations determined in previous investigations. For each predator, consumption rates varied by reservoir area, month, time of day, and predator size or age. The greatest daily consumption of salmonids by northern squawfish and channel catfish occurred in the upper end of the reservoir below McNary Dam. Greatest daily predation by walleyes and smallmouth bass occurred in the middle and lower reservoir. Consumption rates of all predators were highest in July, concurrent with maximum temperature and abundance of juvenile salmonids. Feeding by the predators tended to peak after dawn and near midnight. Northern squawfish below McNary Dam exhibited this pattern, but fed mainly in the morning hours down-reservoir. The daily ration of total prey fish was highest for northern squawfish over 451 mm fork length, for walleyes 201-250 mm, for smallmouth bass 176-200 mm, and for channel catfish 401-450 mm. Averaged over all predator sizes and sampling months (April-August), the total daily ration (fish plus other prey) of smallmouth bass was about twice that of channel catfish, northern squawfish, and walleyes. However, northern squawfish was clearly the major predator on juvenile salmonids

  2. Characteristics of fads2 gene expression and putative promoter in European sea bass (Dicentrarchus labrax): Comparison with salmonid species and analysis of CpG methylation

    OpenAIRE

    Geay, Florian; Zambonino, Jose-luis; Reinhardt, Richard; Kuhl, Heiner; Santigosa, Ester; Cahu, Chantal; Mazurais, David

    2012-01-01

    Marine fish species exhibit low capacity to biosynthesise highly unsaturated fatty acid (HUFA) in comparison to strict freshwater and anadromous species. It is admitted that the Delta(6) desaturase (FADS2) is a key enzyme in the HUFA biosynthetic pathway. We investigated by quantitative PCR the relative amounts of FADS2 mRNA in European sea bass (Dicentrarchus labrax) in comparison with a salmonid species, the rainbow trout (Oncorhynchus mykiss L.). The analysis of the expression data was per...

  3. Potensi Tanaman Ornamental (Aglaonema sp., Dieffenbachia sp., dan Spathiphyllum sp.) dalam Menurunkan Jumlah Mikroba Udara dalam Ruangan Kelas Sekolah Dasar

    OpenAIRE

    Mangunsong, Sisca Nency Teresia

    2016-01-01

    Bioaerosol is dust particles consisting of bacteria and other fungi with spores that are in the room when the temperature and humidity level are adequate. Its presence in the room are generally harmless, but some time causes disease. This research was aims to determine the effect of ornamental plant Aglaonema sp., Dieffenbachia sp., and Spathiphyllum sp. on amount of bacteria and fungi in the class room. Bioaerosol isolation was performed according to the method of air sampling with three rep...

  4. Sebaran dan Kerapatan Populasi Pratylenchus sp dan Radopholus sp Pada Tanaman Kopi (Coffea sp.) Kabupaten Gayo Lues Provinsi Aceh

    OpenAIRE

    Bayhaqi

    2015-01-01

    Bayhaqi, 2014. Distribution and Population Density Of Pratylenchus sp and Radopholus sp on Coffee (Coffea sp.) in Gayo Lues Regency Aceh Province. Under supervising commission Darma Bakti and Hasanuddin. Gayo Coffee is one among the main export commodities of Indonesia has been known in the domestic and international markets, Gayo coffee production includes more than 90% of the total coffee production in the province. Coffee production in Gayo Lues district reached 540 kg / ha with a pla...

  5. DISTRIBUSI Solen sp DI PERAIRAN KABUPATEN BANGKALAN

    Directory of Open Access Journals (Sweden)

    Eva Ari Wahyuni

    2016-03-01

    Full Text Available DISTRIBUTION OF Solen sp IN BANGKALAN WATERSSolen sp potential needs to be developed on the island of Madura, particularly in Bangkalan. Solen sp utilization has increased which has the potential to overfishing. Therefore, this study aims to determine the density of Solen sp and their ecology in the waters Modung village, Modung District, Bangkalan. The experiment was conducted in April 2015 using the descriptive method. The materials used include Solen sp and physico-chemical parameters of the environment (temperature, salinity, pH, and substrate. The analyzes were conducted at the Laboratory of Marine Science, Department of Marine Sciences, Trunojoyo University of Madura by using the tool grabsampler, sieveshaker, and pipetting with gravimetric method. The analysis shows the range of values of temperature between 29-300C, salinity between 31-32 ppt, pH were 7.9-8.0 and the type of substrate in the form of sandy mud, as well as the density of Solen sp from 8-10 individuals/m2. All measurement results indicate normal conditions and in accordance with the sea water quality standard for marine life, which can be a suitable habitat for the growth and development of Solen sp. This condition is thought to affect the density of Solen sp.Keywords: Bangkalan, density, distribution, Solen sp, substrate.ABSTRAKPotensi Solen sp perlu dikembangkan di pulau Madura, khususnya di Kabupaten Bangkalan. Pemanfaatan Solen sp mengalami peningkatan sehingga berpotensi overfishing. Untuk itu, penelitian ini bertujuan untuk mengetahui kepadatan Solen sp dan ekologinya di perairan desa Modung, Kecamatan Modung, Kabupaten Bangkalan. Penelitian dilaksanakan pada bulan April 2015 dengan metode deskriptif. Materi dan bahan yang digunakan diantaranya Solen sp dan parameter fisika-kimia lingkungan (suhu, salinitas, pH, dan substrat. Analisa dilakukan di Laboratorium Ilmu Kelautan, Program studi/Jurusan Ilmu Kelautan Universitas Trunojoyo Madura dengan menggunakan alat

  6. Fungi Association with Cedar’s Seeds and Fusarium sp. and Pestalotia sp. Pathogenicities Levantamento de Fungos em Sementes de Cedro e Avaliação da Patogenicidade de Fusarium sp. e Pestalotia sp.

    Directory of Open Access Journals (Sweden)

    Sílvia Cristina Benetti

    2010-02-01

    Full Text Available

    This research had as main objectives the evaluation of cedar’s seeds seed-borne fungi and the Fusarium
    sp. and Pestalotia sp. pathogenicities. For the fungi detection Potato Dextrose and Agar medium and Blotter
    test methods were used. For the pathogenicity tests, isolates of Fusarium sp. and Pestalotia sp. were used. The following fungi were observed: Pestalotia sp., Fusarium sp., Phomopsis sp., Colletotrichum sp., Macrophomina sp. and Cladosporium sp. The fungi observed in the highest frequencies were: Fusarium sp., Phomopsis sp. and Pestalotia sp. A reduction of the germination level was observed in seedlings from cedar’s seed inoculated with Fusarium sp.

     

    doi: 10.4336/2009.pfb.58.81

    Este trabalho teve por objetivos avaliar fungos em sementes de cedro e testar a patogenicidade de Fusarium sp. e Pestalotia sp. Para detecção de fungos, foram utilizados batata-dextrose-ágar e papel filtro. Para o teste de patogenicidade, utilizaram-se isolados de Fusarium sp. e de Pestalotia sp. Foi constatada a presença dos seguintes fungos nas sementes: Pestalotia sp., Fusarium sp., Phomopsis sp., Colletotrichum sp., Macrophomina sp. e Cladosporium sp. Os fungos de maior freqüência foram: Fusarium sp., Phomopsis sp. e Pestalotia sp. Observou-se decréscimo nos valores de emergência das plântulas oriundas das sementes inoculadas com Fusarium sp.

     

    Doi: 10.4336/2009.pfb.58.81

  7. Anoplastie périnéale simple pour le traitement des malformations anorectales basses chez l'adulte, à propos de deux cas

    Science.gov (United States)

    Echchaoui, Abdelmoughit; Benyachou, Malika; Hafidi, Jawad; Fathi, Nahed; Mohammadine, Elhamid; ELmazouz, Samir; Gharib, Nour-eddine; Abbassi, Abdellah

    2014-01-01

    Les malformations anorectales chez l'adulte sont des anomalies congénitales rares du tube digestif qui prédominent chez le sexe féminin. Notre étude porte sur deux observations de malformation anorectale basses vues et traitées au stade adulte par les 2 équipes (plasticiens et viscéralistes) à l'Hôpital Avicenne à Rabat. Il s'agit d'un homme de 24 ans avec une dyschésie anale l'autre cas est une femme de 18 ans avec une malformation anovulvaire Les caractéristiques cliniques combinées avec les imageries radiologiques (lavement baryté, et la manométrie anorectale) ont confirmé qu'il s'agit d'une malfomation anorectale basse. Les deux cas sont corrigés par une reconstruction sphinctérienne, réimplantation anale avec anoplastie périnéale. Les suites opératoires étaient simples, pas de souffrance cutanée ou nécrose, avec changement de pansement gras chaque jour. Le résultat fonctionnel (la continence) était favorable pour les 2 patients. La présentation des MAR à l’âge adulte est rare, d’étiologie mal connu, elles apparaissent selon le mode sporadique. Les caractéristiques cliniques, couplées à l'imagerie (lavement baryté, IRM pelvienne), l'endoscopie et la manométrie anorectale, permettent de confirmer le diagnostic et classer ces anomalies en 3 types: basses, intermédiaires, et hautes. Les formes basses sont traités d'emblée par une réimplantation anale et anoplastie périnéale simple tels nos deux cas, elles peuvent être traités dans certains cas par un abaissement anorectale associé à une plastie V-Y permettant ainsi un emplacement anatomique correct de l'anus; alors que les formes hautes ou intermédiaires relèvent d'une chirurgie complexe avec souvent une dérivation digestive transitoire. Contrairement aux autres formes, Les formes basses ont un pronostic fonctionnel favorable. PMID:25667689

  8. A decadal trend of juvenile European sea bass (Dicentrarchus labrax, L. responses to climate patterns in the Mondego estuary, Portugal

    Directory of Open Access Journals (Sweden)

    Eduardo Granja Bento

    2015-11-01

    Full Text Available Estuarine systems support the life cycle stages of commercially important marine fish and are influenced by large and local-scale climatic patterns. Also, extreme events triggered by climate changes may influence the functioning of nursery grounds and recruitment for several fish species. In this study, performed in the Mondego estuary, Portugal, we used an 11-year database (2003-2013 for analyzing the variability in the population of a marine juvenile migrant fish, the European sea bass Dicentrarchus labrax, regarding changes in abundance, population structure, growth rates and secondary production and annual day of peak abundance. Higher densities and production occurred at the beginning of the study, but no differences in 0-group growth could be observed. In order to detect change points in both biological and climatic data, the cumulative sum (CUSUM of the deviations from the mean for the 2003-2013 period were determined for each parameter. The relationship between large- and local-scale drivers and 0-group abundance, secondary production and day of peak abundance were evaluated using a Pearson correlation analysis of CUSUM of biological and environmental data, considering the correspondent yearly values and with a time-lag of 1 year. The North Atlantic Oscillation (NAO index, sea surface temperature (SST and their respective winter values were tested as large-scale factors, while river runoff, salinity and water temperature were considered as local climate patterns. River runoff was the significant factor explaining D. labrax 0-group abundances and the NAO and water temperature were also significant predictors considering the 1-year lag. Regarding D. labrax 0-group secondary production, salinity and water temperature were the significant predictors. The NAO with 1-year lag was also negatively correlated with the day of peak abundance. The observed variability regarding yearly trends in abundance of juvenile fish was mostly linked to local

  9. Nodavirus Colonizes and Replicates in the Testis of Gilthead Seabream and European Sea Bass Modulating Its Immune and Reproductive Functions

    Science.gov (United States)

    Valero, Yulema; Arizcun, Marta; Esteban, M. Ángeles; Bandín, Isabel; Olveira, José G.; Patel, Sonal; Cuesta, Alberto; Chaves-Pozo, Elena

    2015-01-01

    Viruses are threatening pathogens for fish aquaculture. Some of them are transmitted through gonad fluids or gametes as occurs with nervous necrosis virus (NNV). In order to be transmitted through the gonad, the virus should colonize and replicate inside some cell types of this tissue and avoid the subsequent immune response locally. However, whether NNV colonizes the gonad, the cell types that are infected, and how the immune response in the gonad is regulated has never been studied. We have demonstrated for the first time the presence and localization of NNV into the testis after an experimental infection in the European sea bass (Dicentrarchus labrax), and in the gilthead seabream (Sparus aurata), a very susceptible and an asymptomatic host fish species, respectively. Thus, we localized in the testis viral RNA in both species using in situ PCR and viral proteins in gilthead seabream by immunohistochemistry, suggesting that males might also transmit the virus. In addition, we were able to isolate infective particles from the testis of both species demonstrating that NNV colonizes and replicates into the testis of both species. Blood contamination of the tissues sampled was discarded by completely fish bleeding, furthermore the in situ PCR and immunocytochemistry techniques never showed staining in blood vessels or cells. Moreover, we also determined how the immune and reproductive functions are affected comparing the effects in the testis with those found in the brain, the main target tissue of the virus. Interestingly, NNV triggered the immune response in the European sea bass but not in the gilthead seabream testis. Regarding reproductive functions, NNV infection alters 17β-estradiol and 11-ketotestosterone production and the potential sensitivity of brain and testis to these hormones, whereas there is no disruption of testicular functions according to several reproductive parameters. Moreover, we have also studied the NNV infection of the testis in vitro to

  10. BANYAN. VII. A New Population of Young Substellar Candidate Members of Nearby Moving Groups from the BASS Survey

    Science.gov (United States)

    Gagné, Jonathan; Faherty, Jacqueline K.; Cruz, Kelle L.; Lafreniére, David; Doyon, René; Malo, Lison; Burgasser, Adam J.; Naud, Marie-Eve; Artigau, Étienne; Bouchard, Sandie; Gizis, John E.; Albert, Loïc

    2015-08-01

    We present the results of a near-infrared (NIR) spectroscopic follow-up survey of 182 M4-L7 low-mass stars and brown dwarfs (BDs) from the BANYAN All-Sky Survey (BASS) for candidate members of nearby, young moving groups (YMGs). We confirm signs of low gravity for 42 new BD discoveries with estimated masses between 8 and 75 {M}{Jup} and identify previously unrecognized signs of low gravity for 24 known BDs. We refine the fraction of low-gravity dwarfs in the high-probability BASS sample to ˜82%. We use this unique sample of 66 young BDs, supplemented with 22 young BDs from the literature, to construct new empirical NIR absolute magnitude and color sequences for low-gravity BDs. We show that low-resolution NIR spectroscopy alone cannot differentiate between the ages of YMGs younger than ˜120 Myr, and that the BT-Settl atmosphere models do not reproduce well the dust clouds in field or low-gravity L-type dwarfs. We obtain a spectroscopic confirmation of low gravity for 2MASS J14252798-3650229, which is a new ˜27 {M}{Jup}, L4 γ bona fide member of AB Doradus. We identify a total of 19 new low-gravity candidate members of YMGs with estimated masses below 13 {M}{Jup}, 7 of which have kinematically estimated distances within 40 pc. These objects will be valuable benchmarks for a detailed atmospheric characterization of planetary-mass objects with the next generation of instruments. We find 16 strong candidate members of the Tucana-Horologium association with estimated masses between 12.5 and 14 {M}{Jup}, a regime where our study was particularly sensitive. This would indicate that for this association there is at least one isolated object in this mass range for every {17.5}-5.0+6.6 main-sequence stellar member, a number significantly higher than expected based on standard log-normal initial mass function, however, in the absence of radial velocity and parallax measurements for all of them, it is likely that this over-density is caused by a number of young interlopers

  11. Gonadotropin Inhibitory Hormone Down-Regulates the Brain-Pituitary Reproductive Axis of Male European Sea Bass (Dicentrarchus labrax).

    Science.gov (United States)

    Paullada-Salmerón, José A; Cowan, Mairi; Aliaga-Guerrero, María; Morano, Francesca; Zanuy, Silvia; Muñoz-Cueto, José A

    2016-06-01

    Gonadotropin-inhibitory hormone (GnIH) inhibits gonadotropin synthesis and release from the pituitary of birds and mammals. However, the physiological role of orthologous GnIH peptides on the reproductive axis of fish is still uncertain, and their actions on the main neuroendocrine systems controlling reproduction (i.e., GnRHs, kisspeptins) have received little attention. In a recent study performed in the European sea bass, we cloned a cDNA encoding a precursor polypeptide that contained C-terminal MPMRFamide (sbGnIH-1) and MPQRFamide (sbGnIH-2) peptide sequences, developed a specific antiserum against sbGnIH-2, and characterized its central and pituitary GnIH projections in this species. In this study, we analyzed the effects of intracerebroventricular injection of sbGnIH-1 and sbGnIH-2 on brain and pituitary expression of reproductive hormone genes (gnrh1, gnrh2, gnrh3, kiss1, kiss2, gnih, lhbeta, fshbeta), and their receptors (gnrhr II-1a, gnrhr II-2b, kiss1r, kiss2r, and gnihr) as well as on plasma Fsh and Lh levels. In addition, we determined the effects of GnIH on pituitary somatotropin (Gh) expression. The results obtained revealed the inhibitory role of sbGnIH-2 on brain gnrh2, kiss1, kiss2, kiss1r, gnih, and gnihr transcripts and on pituitary fshbeta, lhbeta, gh, and gnrhr-II-1a expression, whereas sbGnIH-1 only down-regulated brain gnrh1 expression. However, at different doses, central administration of both sbGnIH-1 and sbGnIH-2 decreased Lh plasma levels. Our work represents the first study reporting the effects of centrally administered GnIH in fish and provides evidence of the differential actions of sbGnIH-1 and sbGnIH-2 on the reproductive axis of sea bass, the main inhibitory role being exerted by the sbGnIH-2 peptide. PMID:26984999

  12. Babesia sp. in Colombian bats (Microchiroptera).

    Science.gov (United States)

    Marinkelle, C J

    1996-07-01

    Two leaf-chinned bats (Mormoops megalophylla) collected in 1963 in central Colombia were heavily infected with Babesia sp., probably Babesia vesperuginis. Both bats had pronounced splenomegaly. This is the first report of a Babesia sp. infection of a bat in the Americas. PMID:8827683

  13. Concentrations of mercury and other metals in black bass (Micropterus spp.) from Whiskeytown Lake, Shasta County, California, 2005

    Science.gov (United States)

    May, Jason T.; Hothem, Roger L.; Bauer, Marissa L.; Brown, Larry R.

    2012-01-01

    This report presents the results of a reconnaissance study conducted by the U.S. Geological Survey (USGS) to determine mercury (Hg) and other selected metal concentrations in Black bass (Micropterus spp.) from Whiskeytown Lake, Shasta County, California. Total mercury concentrations were determined by cold-vapor atomic absorption spectroscopy (CVAAS) in fillets and whole bodies of each sampled fish. Selected metals scans were performed on whole bodies (less the fillets) by inductively coupled plasma–mass spectroscopy (ICP-MS) and inductively coupled plasma–optical emission spectroscopy (ICP-OES). Mercury concentrations in fillet samples ranged from 0.06 to 0.52 micrograms per gram (μg/g) wet weight (ww). Total mercury (HgT) in the same fish whole-body samples ranged from 0.04 to 0.37 (μg/g, ww). Mercury concentrations in 17 percent of "legal catch size" (≥305 millimeters in length) were above the U.S. Environmental Protection Agency water-quality criterion for the protection of human health of 0.30 μg/g (ww). These data will serve as a baseline for future monitoring efforts within Whiskeytown Lake.

  14. Fatty acid profile of sunshine bass: I. Profile change is affected by initial composition and differs among tissues.

    Science.gov (United States)

    Trushenski, Jesse T; Lewis, Heidi A; Kohler, Christopher C

    2008-07-01

    Fatty acid (FA) composition of fillet tissue can be tailored by transitioning fish from alternative lipid-based, low long-chain polyunsaturated fatty acid (LC-PUFA) grow-out feeds to high LC-PUFA "finishing" feeds. To address whether grow-out feed composition influences the responsiveness of fillet tissue to finishing, sunshine bass (SB, Morone chrysops x M. saxatilis) were reared to a submarketable size on grow-out feeds containing fish oil (FO) or a 50:50 blend of FO and coconut (CO), grapeseed (GO), linseed (LO), or poultry (PO) oil. For the final 8 weeks of the trial, fish were either maintained on assigned grow-out feeds or finished with the 100% FO feed. Production performance was unaffected by dietary lipid source, but fillet FA profile generally conformed to nutritional history. Regardless of grow-out regimen, finishing had a significant restorative effect on fillet FA composition; however, complete restoration of control levels of 20:5n-3, 22:6n-3, total LC-PUFA and n-3:n-6 FA ratio was achieved only among fish fed the CO-based grow-out feed. Saturated fatty acids (SFA) appear to be preferential catabolic substrates, whereas medium-chain and long-chain PUFA are selectively deposited in tissues. Provision of SFA in grow-out feeds appears to optimize selective FA metabolism and restoration of beneficial fillet FA profile during finishing. PMID:18521646

  15. Mercury in fish scales as an assessment method for predicting muscle tissue mercury concentrations in largemouth bass.

    Science.gov (United States)

    Lake, J L; Ryba, S A; Serbst, J R; Libby, A D

    2006-05-01

    The relationship between total mercury (Hg) concentration in fish scales and in tissues of largemouth bass (Micropterus salmoides) from 20 freshwater sites was developed and evaluated to determine whether scale analysis would allow a nonlethal and convenient method for predicting Hg concentrations in tissues. The relationship between total Hg concentration in untreated scale samples and muscle tissue is highly variable. Several different scale treatments were tried in an effort to increase the coefficient of determination and thereby enhance the effectiveness of this predictive technique. Washing scales with acetone, deionized (DI) water, detergent solution, and soap were used in conjunction with ultrasonication. The use of a mild soap solution with heating and ultrasonication increased the r(2) the most (from 0.69 [untreated scales] to 0.89). However, despite treatment, wide predictions of tissue Hg concentration remained. These results suggest that application of this technique as an independent method for issuance of fish advisories is inappropriate. Nevertheless, our results showed that scale analysis has potential for assessing general trends in concentration relative to a tissue criterion and for assessing Hg contamination in fish tissue as a first-level screen. PMID:16435082

  16. Does virulence assessment of Vibrio anguillarum using sea bass (Dicentrarchus labrax larvae correspond with genotypic and phenotypic characterization?

    Directory of Open Access Journals (Sweden)

    Ingeborg Frans

    Full Text Available BACKGROUND: Vibriosis is one of the most ubiquitous fish diseases caused by bacteria belonging to the genus Vibrio such as Vibrio (Listonella anguillarum. Despite a lot of research efforts, the virulence factors and mechanism of V. anguillarum are still insufficiently known, in part because of the lack of standardized virulence assays. METHODOLOGY/PRINCIPAL FINDINGS: We investigated and compared the virulence of 15 V. anguillarum strains obtained from different hosts or non-host niches using a standardized gnotobiotic bioassay with European sea bass (Dicentrarchus labrax L. larvae as model hosts. In addition, to assess potential relationships between virulence and genotypic and phenotypic characteristics, the strains were characterized by random amplified polymorphic DNA (RAPD and repetitive extragenic palindromic PCR (rep-PCR analyses, as well as by phenotypic analyses using Biolog's Phenotype MicroArray™ technology and some virulence factor assays. CONCLUSIONS/SIGNIFICANCE: Virulence testing revealed ten virulent and five avirulent strains. While some relation could be established between serotype, genotype and phenotype, no relation was found between virulence and genotypic or phenotypic characteristics, illustrating the complexity of V. anguillarum virulence. Moreover, the standardized gnotobiotic system used in this study has proven its strength as a model to assess and compare the virulence of different V. anguillarum strains in vivo. In this way, the bioassay contributes to the study of mechanisms underlying virulence in V. anguillarum.

  17. Ovary transcriptome profiling via artificial intelligence reveals a transcriptomic fingerprint predicting egg quality in striped bass, Morone saxatilis.

    Science.gov (United States)

    Chapman, Robert W; Reading, Benjamin J; Sullivan, Craig V

    2014-01-01

    Inherited gene transcripts deposited in oocytes direct early embryonic development in all vertebrates, but transcript profiles indicative of embryo developmental competence have not previously been identified. We employed artificial intelligence to model profiles of maternal ovary gene expression and their relationship to egg quality, evaluated as production of viable mid-blastula stage embryos, in the striped bass (Morone saxatilis), a farmed species with serious egg quality problems. In models developed using artificial neural networks (ANNs) and supervised machine learning, collective changes in the expression of a limited suite of genes (233) representing 90% of the eventual variance in embryo survival. Egg quality related to minor changes in gene expression (networks are centrally involved in regulation of early development in all vertebrates, including humans. By assessing collective levels of the relevant ovarian transcripts via ANNs we were able, for the first time in any vertebrate, to accurately predict the subsequent embryo developmental potential of eggs from individual females. Our results show that the transcriptomic fingerprint evidencing developmental dysfunction is highly predictive of, and therefore likely to regulate, egg quality, a biologically complex trait crucial to reproductive fitness. PMID:24820964

  18. Effects of electro-fishing on galvano-taxis and carcass quality characteristics in sea bass (Dicentrarchus labrax

    Directory of Open Access Journals (Sweden)

    Edo D’Agaro

    2010-01-01

    Full Text Available The aim of this study was to investigate the effects of electro-fishing in sea water. We evaluated the feasibility of an electro-fishing system using numerical simulations for laboratory tanks and the open sea and performing a laboratory experiment. A non-homogeneous bi-dimensional electric-field model for marine water and fish based on discrete formulation of electro-magnetic field equations was developed using GAME (geometric approach for Maxwell equations software. Voltage gradients inside the fish and close to the body were determined. Re- sults showed that fish in the open sea and in groups had greater internal voltage differences than did fish in tanks and single fish. Sea bass (length:10 and 30 cm were exposed in laboratory tanks to pulsed direct current (PDC, 25-125 Hz and duty cycle (5-40%. We measured the electro-taxis and tetanus thresholds after electrical exposure. It is significant that these values decreased with increasing the size of fish. No differences were found after electro-fishing on overall appearance, internal and external haemorrhage, standard freshness scoring techniques and carcass quality characteristics

  19. The C-Band All-Sky Survey (C-BASS): Design and implementation of the northern receiver

    CERN Document Server

    King, O G; Blackhurst, E J; Copley, C; Davis, R J; Dickinson, C; Holler, C M; Irfan, M O; John, J J; Leahy, J P; Leech, J; Muchovej, S J C; Pearson, T J; Stevenson, M A; Taylor, Angela C

    2013-01-01

    The C-Band All-Sky Survey (C-BASS) is a project to map the full sky in total intensity and linear polarization at 5 GHz. The northern component of the survey uses a broadband single-frequency analogue receiver fitted to a 6.1-m telescope at the Owens Valley Radio Observatory in California, USA. The receiver architecture combines a continuous-comparison radiometer and a correlation polarimeter in a single receiver for stable simultaneous measurement of both total intensity and linear polarization, using custom-designed analogue receiver components. The continuous-comparison radiometer measures the temperature difference between the sky and temperature-stabilized cold electrical reference loads. A cryogenic front-end is used to minimize receiver noise, with a system temperature of $\\approx 30$ K in both linear polarization and total intensity. Custom cryogenic notch filters are used to counteract man-made radio frequency interference. The radiometer $1/f$ noise is dominated by atmospheric fluctuations, while th...

  20. Drospirenone intake alters plasmatic steroid levels and cyp17a1 expression in gonads of juvenile sea bass.

    Science.gov (United States)

    Blanco, Maria; Fernandes, Denise; Medina, Paula; Blázquez, Mercedes; Porte, Cinta

    2016-06-01

    Drospirenone (DRO) is one of the most widely used progestins in contraceptive treatments and hormone replacement therapies. The pharmacokinetics and potential toxicological effects of DRO were investigated in juvenile sea bass (Dicentrarchus labrax) exposed through the diet (0.01-10 μg DRO/g) for up to 31 days. DRO was detected in the blood (4-27 ng/mL) of fish exposed to the highest concentration, with no significant bioaccumulation over time and no alteration of hepatic metabolizing enzymes, namely, CYP1A and CYP3A-catalysed activities and UDP-glucuronyltransferase (UGT). Pregnenolone (P5), progesterone (P4), 17α-hydroxyprogesterone (17P4), 17α-hydroxypregnenolone (17P5), androstenedione (AD) and testosterone (T) were determined in plasma and gene expression of cyp17a1, cyp19a1a and cyp11β analysed by qRT-PCR in gonads. The significant increase in plasmatic levels of 17P5, 17P4 and AD detected after 31 days exposure to 10 ng DRO/g together with the increased expression of cyp17a1 in females evidence the ability of DRO to alter steroid synthesis at low intake concentrations (7 ng DRO/day). However, the potential consequences of this steroid shift for female reproduction remain to be investigated. PMID:26995450

  1. Evaluation of heavy metal uptake in micropterus salmoides (Largemouth Bass) of Lake Austin, TX by neutron activation analysis

    International Nuclear Information System (INIS)

    Neutron activation analysis was used to investigate and quantify the level of heavy metal uptake in the marine environment of Lake Austin in Austin, TX. Specifically, the samples studied were largemouth bass, or micropterus salmoides. The presence of heavy metals in the food chain presents multiple hazards, mostly as a food hazard for those species that ingest the fish, namely humans. To measure the concentrations of heavy metals in various fish samples, the nuclear analytical technique of neutron activation analysis (NAA) was used. Both epithermal and thermal irradiations were conducted for the NAA to look for short and long-lived radioisotopes, respectively. The samples themselves consisted of liver and tissue samples for each of the fish caught. Each sample was freeze-dried and homogenized before irradiation and spectrum acquisition. The results showed that all levels of heavy metals were not sufficient enough to make the fish unsafe for eating, with the highest levels being found for iron and zinc. Gold was found to be at much higher concentrations in the younger fish and virtually non-existent in the larger of the samples. (author)

  2. Impact of chronic cadmium exposure at environmental dose on escape behaviour in sea bass (Dicentrarchus labrax L.; Teleostei, Moronidae)

    International Nuclear Information System (INIS)

    The effect of chronic exposure to a low concentration (0.5 μg l-1) of cadmium ions was investigated on escape behaviour of sea bass, Dicentrarchus labrax, using video analysis. Observations were also performed on the microanatomy of lateral system neuromasts. When fish were exposed for 4 h per day over 8 days to the cadmium ions, most of both types of neuromasts observed remained intact. However, some of them presented damaged sensory maculae. Whereas before cadmium exposure, fish responded positively to nearly all the lateral system stimulations, after exposure they decreased by about 10% their positive responses to stimulations. From the 15th day after the beginning of cadmium exposure, neuromasts presented progressively less damage, cadmium accumulation in gills and scales decreased significantly and fish escape behaviour had recovered. This study presents a new concept in ecotoxicology: using behavioural change to reveal the effects of pollution levels, scarcely detectable by currently used techniques (physiological responses). - Cadmium exposure involved a significant bioaccumulation in fish scales, slight damage to the lateral line system and a significant decrease in fish escape behaviour

  3. Glyphosate catabolism by Pseudomonas sp

    International Nuclear Information System (INIS)

    The pathway for the degradation of glyphosate (N-phosphonomethylglycine) by Pseudomonas sp. PG2982 has been determined using metabolic radiolabeling experiments. Radiorespirometry experiments utilizing [3-14C] glyphosate revealed that approximately 50-59% of the C3 carbon was oxidized to CO2. Fractionation of stationary phase cells labeled with [3-14C]glyphosate revealed that from 45-47% of the assimilated C3 carbon is distributed to proteins and that amino acids methionine and serine are highly labeled. The nucleic acid bases adenine and guanine received 90% of the C3 label that was incorporated into nucleic acids, and the only pyrimidine base labeled was thymine. Pulse labeling of PG2982 cells with [3-14C]glyphosate revealed that [3-14C]sarcosine is an intermediate in glyphosate degradation. Examination of crude extracts prepared from PG2982 cells revealed the presence of an enzyme that oxidizes sarcosine to glycine and formaldehyde. These results indicate that the first step in glyphosate degradation by PG2982 is cleavage of the carbon-phosphorus bond, resulting in the release of sarcosine and a phosphate group. The phosphate group is utilized as a source of phosphorus, and the sarcosine is degraded to glycine and formaldehyde. Phosphonate utilization by Pseudomonas sp. PG2982 was investigated. Each of the ten phosphonates tested were utilized as a sole source of phosphorus by PG2982. Representative compounds tested included alkylphosphonates, 1-amino-substituted alkylphosphonates, amino-terminal phosphonates, and an arylphosphonate. PG2982 cultures degraded phenylphosphonate to benzene and produced methane from methylphosphonate. The data indicate that PG2982 is capable of cleaving the carbon-phosphorus bond of several structurally different phosphonates

  4. Predictive value of Sp1/Sp3/FLIP signature for prostate cancer recurrence.

    Directory of Open Access Journals (Sweden)

    Roble G Bedolla

    Full Text Available Prediction of prostate cancer prognosis is challenging and predictive biomarkers of recurrence remain elusive. Although prostate specific antigen (PSA has high sensitivity (90% at a PSA level of 4.0 ng/mL, its low specificity leads to many false positive results and considerable overtreatment of patients and its performance at lower ranges is poor. Given the histopathological and molecular heterogeneity of prostate cancer, we propose that a panel of markers will be a better tool than a single marker. We tested a panel of markers composed of the anti-apoptotic protein FLIP and its transcriptional regulators Sp1 and Sp3 using prostate tissues from 64 patients with recurrent and non-recurrent cancer who underwent radical prostatectomy as primary treatment for prostate cancer and were followed with PSA measurements for at least 5 years. Immunohistochemical staining for Sp1, Sp3, and FLIP was performed on these tissues and scored based on the proportion and intensity of staining. The predictive value of the FLIP/Sp1/Sp3 signature for clinical outcome (recurrence vs. non-recurrence was explored with logistic regression, and combinations of FLIP/Sp1/Sp3 and Gleason score were analyzed with a stepwise (backward and forward logistic model. The discrimination of the markers was identified by sensitivity-specificity analysis and the diagnostic value of FLIP/Sp1/Sp3 was determined using area under the curve (AUC for receiver operator characteristic curves. The AUCs for FLIP, Sp1, Sp3, and Gleason score for predicting PSA failure and non-failure were 0.71, 0.66, 0.68, and 0.76, respectively. However, this increased to 0.93 when combined. Thus, the "biomarker signature" of FLIP/Sp1/Sp3 combined with Gleason score predicted disease recurrence and stratified patients who are likely to benefit from more aggressive treatment.

  5. PENGARUH FILTRAT CENDAWAN Aspergillus sp. DAN Fusarium sp. TERHADAP VIABILITAS BENIH DAN PERTUMBUHAN BIBIT SENGON (Paraserianthes falcataria)

    OpenAIRE

    Naning Yuniarti; Tati Suharti; Yulianti Bramasto

    2013-01-01

    Fungi are one of the main causes of damage to seeds. Fungi can be a pathogen or saprophyte such as Aspergillus sp. and Fusarium sp. The purpose of this study was to determine the effects of filtrate fungus Aspergillus sp and Fusarium sp on seed viability and seedling growth performance of Sengon (Paraserianthes falcataria). The experiment design used was the Completely Randomized Design (CRD), with treatment: control, filtrate of Aspergillus sp and Fusarium sp filtrate. Each treatment was rep...

  6. Fresh Water Cyanobacteria Geitlerinema sp. CCC728 and Arthrospira sp. CCC729 as an Anticancer Drug Resource

    OpenAIRE

    Akanksha Srivastava; Ratnakar Tiwari; Vikas Srivastava; Tej Bali Singh; Ravi Kumar Asthana

    2015-01-01

    An increasing number of cancer patients worldwide, especially in third world countries, have raised concern to explore natural drug resources, such as the less explored fresh water filamentous cyanobacteria. Six strains of cyanobacteria (Phormidium sp. CCC727, Geitlerinema sp. CCC728, Arthrospira sp. CCC729, Phormidium sp. CCC731, Phormidium sp. CCC730, and Leptolyngbya sp. CCC732) were isolated (paddy fields and ponds in the Banaras Hindu University, campus) and five strains screened for ant...

  7. Application d'un modèle de dynamique de population de truite commune (Salmo trutta sur un bassin de Basse-Normandie : premières simulations

    Directory of Open Access Journals (Sweden)

    GOURAUD V.

    1998-07-01

    Full Text Available Afin d'évaluer l'impact des différents usages de l'eau sur l'écosystème aquatique, des outils de simulation de plus en plus nombreux voient actuellement le jour ; dans ce cadre, un modèle de dynamique de population de truite (Salmo trutta est en cours de développement. Pour enrichir les nombreuses observations déjà disponibles sur le bassin de l'Oir (Basse-Normandie, des inventaires assortis d'expérimentations spécifiques ont été effectués en 1996 pour conforter et compléter les différentes composantes du modèle, en particulier en ce qui concerne les fonctions biologiques impliquées. En particulier, l'étude de l'habitat permet de déterminer la capacité d'accueil du milieu et ainsi une composante densité-dépendante de la survie des jeunes truitelles ; le potentiel reproducteur est appréhendé à l'aide de prélèvements sanguins et du sacrifice de quelques femelles matures. D'éventuels échanges avec l'aval du bassin sont quantifiés à l'aide d'un système de piégeage. Les résultats de ces expérimentations permettent d'effectuer une première application sur l'ensemble du bassin et de comparer les simulations obtenues aux données issues des inventaires piscicoles. L'utilisation d'un pas de temps de calcul hebdomadaire semble plus appropriée qu'un pas de temps mensuel pour représenter l'effet de la température sur la population. Cependant, le modèle dans son état actuel ne permet pas de représenter de façon satisfaisante l'impact du fort accident climatique observé en été 91 ; il donne par contre une bonne image de la restauration de la population de géniteurs à partir de 1992.

  8. Sp1 regulates human huntingtin gene expression.

    Science.gov (United States)

    Wang, Ruitao; Luo, Yawen; Ly, Philip T T; Cai, Fang; Zhou, Weihui; Zou, Haiyan; Song, Weihong

    2012-06-01

    Huntington's disease (HD) is a hereditary neurodegenerative disorder resulting from the expansion of a polyglutamine tract in the huntingtin protein. The expansion of cytosine-adenine-guanine repeats results in neuronal loss in the striatum and cortex. Mutant huntingtin (HTT) may cause toxicity via a range of different mechanisms. Recent studies indicate that impairment of wild-type HTT function may also contribute to HD pathogenesis. However, the mechanisms regulating HTT expression have not been well defined. In this study, we cloned 1,795 bp of the 5' flanking region of the human huntingtin gene (htt) and identified a 106-bp fragment containing the transcription start site as the minimal region necessary for promoter activity. Sequence analysis reveals several putative regulatory elements including Sp1, NF-κB, HIF, CREB, NRSF, P53, YY1, AP1, and STAT in the huntingtin promoter. We found functional Sp1 response elements in the huntingtin promoter region. The expression of Sp1 enhanced huntingtin gene transcription and the inhibition of Sp1-mediated transcriptional activation reduced huntingtin gene expression. These results suggest that Sp1 plays an important role in the regulation of the human huntingtin gene expression at the mRNA and protein levels. Our study suggests that the dysregulation of Sp1-mediated huntingtin transcription, combining with mutant huntingtin's detrimental effect on other Sp1-mediated downstream gene function, may contribute to the pathogenesis of HD. PMID:22399227

  9. Production and characterization of recombinantly derived peptides and antibodies for accurate determinations of somatolactin, growth hormone and insulin-like growth factor-I in European sea bass (Dicentrarchus labrax).

    Science.gov (United States)

    de Celis, S Vega-Rubín; Gómez-Requeni, P; Pérez-Sánchez, J

    2004-12-01

    A specific radioimmunoassay (RIA) for European sea bass (Dicentrarchus labrax) growth hormone (GH) was developed and validated. For this purpose, a stable source of GH was produced by means of recombinant DNA technology in a bacteria system. The identity of the purified protein (ion exchange chromatography) was demonstrated by Western blot and a specific GH antiserum was raised in rabbit. In Western blot and RIA system, this antiserum recognized specifically native and recombinant GH, and it did not cross-react with fish prolactin (PRL) and somatolactin (SL). In a similar way, a specific polyclonal antiserum against the now available recombinant European sea bass SL was raised and used in the RIA system to a sensitivity of 0.3 ng/ml (90% of binding of tracer). Further, European sea bass insulin-like growth factor-I (IGF-I) was cloned and sequenced, and its high degree of identity with IGF-I peptides of barramundi, tuna, and sparid fish allowed the use of a commercial IGF-I RIA based on barramundi IGF-I antiserum. These assay tools assisted for the first time accurate determinations of SL and GH-IGF-I axis activity in a fish species of the Moronidae family. Data values were compared to those found with gilthead sea bream (Sparus aurata), which is currently used as a Mediterranean fish model for growth endocrinology studies. As a characteristic feature, the average concentration year round of circulating GH in growing mature males of European sea bass was higher than in gilthead sea bream. By contrast, the average concentration of circulating SL was lower. Concerning to circulating concentration of IGF-I, the measured plasma values for a given growth rate were also lower in European sea bass. These findings are discussed on the basis of a different energy status that might allowed a reduced but more continuous growth in European sea bass. PMID:15560873

  10. Safradan İzole Edilen Campylobacter Sp.

    OpenAIRE

    Yorgancıgil, Birdal; Tezeren, Deniz; Balaban, Neriman; Terzioğlu, Serdar; Öztürk, Süheyla

    2009-01-01

    SüleymanDemirel Üniversitesi TIP FAKÜLTESİ DERGİSİ: 1997 Haziran; 4(3) Safradan İzole Edilen Campylobacter Sp. Birdal YORGANCIGİL Deniz TEZEREN Neriman BALABAN Serdar TERZlOĞLU Süheyla ÖZTÜRK Özet Campylobacter sp. 'in neden olduğu, gastrointestinal traktüs haricindeki lokal veya sistemik infeksiyon vakaları oldukça azdır. Bu çalışmada safradan izole edilen bir Campylobacter sp. olgusu incelenmiştir. Ankara Numune Hastanesi Cerrahi Kliniğinde koles...

  11. EVALUACIÓN DE LAS TÉCNICAS DE CEPILLADO DE BASS Y VERTICAL EN UN GRUPO DE PACIENTES EN TRATAMIENTO DE ORTODONCIA CON TÉCNICA MBT

    Directory of Open Access Journals (Sweden)

    Yelitza Ivone Baracaldo Ortíz

    2012-12-01

    Full Text Available Objetivo: Evaluar la efectividad de las técnicas de Cepillado de Bass y Vertical en pacientes con aparatología ortodóntica fija tratados con la técnica MBT. Materiales y métodos: se realizó un estudio experimental “In vivo” aleatorizado doble ciego; la muestra fue de 100 personas distribuidas en dos grupos, a cada uno se le asignó una Técnica de Cepillado y se dieron instrucciones de higiene oral por medio de charlas personalizadas y por escrito. Se usó el índice de Sillnes y Löe para medir el porcentaje de placa bacteriana, el cual fue realizado por una higienista oral, cada 30 ± 3 días durante 3 meses. A los dos grupos se les realizó profilaxis con el objetivo de tener un índice inicial de placa lo más aproximado a cero. A los participantes se les entregó mensualmente los aditamentos de higiene oral que debían usar. Resultados: antes de dar instrucciones de higiene oral el 73,4% tenían índice de placa moderado, el 16,46% severo y el 10,3% incipiente. El grupo de Técnica Vertical obtuvo mayor reducción de placa en comparación con el grupo de Técnica  Bass durante la primera toma (pConclusiones: La Técnica de Cepillado Vertical es más efectiva que la Técnica Bass para disminuir la placa bacteriana en pacientes tratados ortodónticamente con la técnica MBT.  

  12. Is the kisspeptin system involved in responses to food restriction in order to preserve reproduction in pubertal male sea bass (Dicentrarchus labrax)?

    Science.gov (United States)

    Escobar, Sebastián; Felip, Alicia; Zanuy, Silvia; Carrillo, Manuel

    2016-09-01

    Previous works on European sea bass have determined that long-term exposure to restrictive feeding diets alters the rhythms of some reproductive/metabolic hormones, delaying maturation and increasing apoptosis during gametogenesis. However, exactly how these diets affect key genes and hormones on the brain-pituitary-gonad (BPG) axis to trigger puberty is still largely unknown. We may hypothesize that all these signals could be integrated, at least in part, by the kisspeptin system. In order to capture a glimpse of these regulatory mechanisms, kiss1 and kiss2 mRNA expression levels and those of their kiss receptors (kiss1r, kiss2r) were analyzed in different areas of the brain and in the pituitary of pubertal male sea bass during gametogenesis. Furthermore, other reproductive hormones and factors as well as the percentage of males showing full spermiation were also analyzed. Treated fish fed maintenance diets provided evidence of overexpression of the kisspeptin system in the main hypophysiotropic regions of the brain throughout the entire sexual cycle. Conversely, Gnrh1 and gonadotropin pituitary content and plasma sexual steroid levels were downregulated, except for Fsh levels, which were shown to increase during spermiation. Treated fish exhibited lower rates of spermiation as compared to control group and a delay in its accomplishment. These results demonstrate how the kisspeptin system and plasma Fsh levels are differentially affected by maintenance diets, causing a retardation, but not a full blockage of the reproductive process in the teleost fish European sea bass. This suggests that a hormonal adaptive strategy may be operating in order to preserve reproductive function in this species. PMID:27164487

  13. Molecular characterization and in vivo expression of hypoxia inducible factor (HIF-1α in sea bass (Dicentrarchus labrax exposed to acute and chronic hypoxi

    Directory of Open Access Journals (Sweden)

    Marco Saroglia

    2010-01-01

    Full Text Available Aquatic hypoxia is a frequent event and in fish a complex set of physiological and bi- ochemical alterations are employed to cope with this environmental stress. Many of these adjustments depend to a large extent on changes in the expression of genes that encode for physiologically relevant proteins. Genes that are induced by hypoxia appear to share a common mode of transcriptional regula- tion. This induction depends upon activation of a transcription factor, the hypoxia inducible factor-1 (HIF-1, which is a heterodimer composed of two subunits: α ‧ and β. In this study we report first on the molecular cloning and characterization of HIF-1α ‧ in sea bass (Dicentrarchus labrax. The full-length sea bass cDNA for HIF-1α was isolated and deposited in the GenBank with accession no. DQ171936. It consists of 3317 base pairs (bp carrying a single open-read- ing frame that encompasses 2265 bp of the coding region and 1052 bp of the 3’ UTR. We then utilized the real-time PCR technology to monitor dynamic changes in levels of HIF-1α ‧ tran- scripts, in response to acute and chronic hypoxic stress. The number of HIF-1α ‧ mRNA copies were signifi- cantly increased in response to both acute (1.9 mg/L, dissolved oxygen for 4 hours and chronic (4.3 mg/L, DO for 15 days hypoxia in sea bass, whereas it remained unchanged in fish exposed to hyperoxic (DO 13.5±1.2 mg/L, 155 % saturation conditions. This is the first study carried out to investigate the behaviour of HIF-1α gene transcripts during hypoxia in representative of marine hypoxia-sensitive fish species.

  14. Detection of an endocrine disrupter biomarker, vitellogenin, in largemouth bass serum using AlGaN/GaN high electron mobility transistors

    Science.gov (United States)

    Chu, Byung Hwan; Chang, C. Y.; Kroll, Kevin; Denslow, Nancy; Wang, Yu-Lin; Pearton, S. J.; Dabiran, A. M.; Wowchak, A. M.; Cui, B.; Chow, P. P.; Ren, Fan

    2010-01-01

    Endocrine disrupters are known to have negative effects on the environment and human health. Real time detection of vitellogenin, an endocrine disrupter biomarker, was demonstrated using AlGaN/GaN high electron mobility transistors (HEMTs). Anti-vitellogenin antibodies were chemically anchored to the gold-coated gate area of the HEMT and immobilized with thioglycolic acid. The potential difference that occurs from the vitellogenin antigen-antibody interaction-induced caused a drain current change in the HEMT. The HEMT sensor was tested for vitellogenin detection both in phosphate buffer saline and largemouth bass serum.

  15. MORPHOLOGICAL AND ELECTROPHYSIOLOGICAL STUDIES OF HORIZONTAL CELLS IN THE BLACK BASS RETINA(Issue Commemorating the Directorship of Prof. Yoko HASHIMOTO, at the Department of Physiology)

    OpenAIRE

    UMINO, Osamu; MAEHARA, Michiyo; HASHIMOTO, Yoko; 海野, 修; 前原, 通代; 橋本, 葉子

    1997-01-01

    The purpose of this study is to examine the correlation between the morphological characteristics and spectral responses of horizontal cells in black bass retina. Three layers of horizontal cell bodies and one layer of fine processes of horizontal cell axons were recognized in the horizontal cell layer. The most distally layered H1 cells were smaller than the H2 cells which form the second layer. Both H1 and H2 cells showed a monophasic spectral response with a maximum peak for red light. In ...

  16. Impact of acute stress on antimicrobial polypeptides mRNA copy number in several tissues of marine sea bass (Dicentrarchus labrax

    Directory of Open Access Journals (Sweden)

    Terova Genciana

    2011-12-01

    Full Text Available Abstract Background In comparison to higher vertebrates, fish are thought to rely heavily on innate immune system for initial protection against pathogen invasion because their acquired immune system displays a considerably poor immunological memory, and short-lived secondary response. The endogenous antimicrobial polypeptides (AMPPs directly and rapidly killing pathogens such as bacteria, fungi, parasites, and viruses are included within the realm of innate defenses. In addition to piscidins, AMPPs that in recent years have been shown to be commonly linked to innate defense, are histones and their polypeptide fragments, and peptides derived from the respiratory protein hemoglobin. There is evidence that a number of stresses lead to significant regulation of AMPPs and thus their monitoring could be a highly sensitive measure of health status and risk of an infectious disease outbreak, which is a major impediment to the continued success of virtually all aquaculture enterprises and is often the most significant cause of economic losses. Results We firstly isolated and deposited in Genbank database the cDNA sequences encoding for hemoglobin-β-like protein (Hb-LP [GeneBank: JN410659], H2B histone-like protein 1 (HLP1 GenBank: JN410660], and HLP2 [GenBank: JN410661]. The "de novo" prediction of the three-dimensional structures for each protein is presented. Phylogenetic trees were constructed on Hb-LP, HLP1, and HLP2 sequences of sea bass and those of other teleost, avian, reptiles, amphibian and mammalian species. We then used real time RT-PCR technology to monitor for the first time in sea bass, dynamic changes in mRNA copy number of Hb-LP, HLP1, HLP2, and dicentracin in gills, skin, eyes, stomach and proximal intestine in response to acute crowding/confinement stress. We showed that acute crowding stress induces an increase in the expression levels of the aforementioned genes, in gills and skin of sea bass, but not in other tissues, and that

  17. Studies on the nutrition of spotted sand bass, Paralabrax maculatofasciatus. Effect of the dietary protein level on growth and protein utilization in juveniles fed semipurified diets

    OpenAIRE

    Anguas Vélez, Benjamín H.; Civera Cerecedo, Roberto; Cadena Roa, Marco; Guillaume, Juan; Martínez Díaz, Sergio Francisco

    2000-01-01

    Two feeding trials were conducted to determine the digestibility of a casein-based semi­purified diet and the effects of different protein levels on growth and protein use of spotted sand bass Paralabrax maculatofascÜltUs juveniles. For trial 1, a semipurified diet with vitamin­free casein as the sole source of protein was fed three times a day to apparent satiation, for a period of 20 d. Feces were collected by siphoning each tank. The digestibility of the exper­imental diet was ...

  18. Low frequency sound field control in rectangular listening rooms using CABS (Controlled Acoustic Bass System) will also reduce sound transmission to neighbor rooms

    DEFF Research Database (Denmark)

    Nielsen, Sofus Birkedal; Celestinos, Adrian

    2011-01-01

    Sound reproduction is often taking place in small and medium sized rectangular rooms. As rectangular rooms have 3 pairs of parallel walls the reflections at especially low frequencies will cause up to 30 dB spatial variations of the sound pressure level in the room. This will take place not only at...... resonance frequencies, but more or less at all frequencies. A time based room correction system named CABS (Controlled Acoustic Bass System) has been developed and is able to create a homogeneous sound field in the whole room at low frequencies by proper placement of multiple loudspeakers. A normal setup...

  19. Dominant Aerobic Bacterial Community of Sea Bass (Dicentrarchus Labrax L.1758) Larvae During Weaning from Artemia to Dry Feed in Culture Conditions

    OpenAIRE

    AYAZ, Ayberk; KARATAŞ*, Süheyla

    2010-01-01

    The aim of this study was to determine the dominant aerobic bacterial community of sea bass larvae during their weaning period in 2 different marine hatcheries in Muğla-Bodrum region. Samplings were made in January, March, and May 2005. Larvae and water samples were diluted in certain ratios and were inoculated to Marine Agar, TCBS Agar, Pseudomonas Agar, King Bee Agar, MRS agar, Blood Agar and Plate Count Agar, in that order. Water temperature, pH, saturation, salinity, and features of the s...

  20. Modification of fillet composition and evidence of differential fatty acid turnover in sunshine bass Morone chrysops x M. saxatilis following change in dietary lipid source.

    Science.gov (United States)

    Lane, Ryan L; Trushenski, Jesse T; Kohler, Christopher C

    2006-11-01

    Marine oil-based finishing diets have been used to restore fillet FA profile in several "medium-fat" fleshed aquaculture species, and a simple dilution model describing FA turnover has been established to predict and tailor final fillet composition. We evaluated finishing diet efficacy and suitability of the dilution model to describe patterns of FA change in a lean-fleshed model, sunshine bass. Two practical diets (45% crude protein, 15% crude lipid) were formulated, respectively containing corn oil (CO) or menhaden oil (MO) as the primary lipid sources. Sunshine bass (age 1 [approximately 14 mo], 347 +/- 8.6 g, mean individual weight +/- SEM) were stocked in a recirculating system and fed the diets according to different feeding regimens during the final 28 wk of the production cycle. Control groups were fed the CO or the MO feeds exclusively; whereas, the remaining treatment groups were transitioned from the CO diet to the MO diet at 4-, 8-, or 12-wk intervals. Upon completion of the feeding trial, fish were harvested, and production performance and fillet composition were assessed. Replacing MO with CO as the primary lipid source in sunshine bass diets yielded fillets with distinctly different FA profiles; however, finishing with a MO-based diet offered significant compensation for CO-associated reductions in fillet long-chain highly unsaturated FA (LC-HUFA). Although complete restoration was not observed, we achieved significant augmentation of endogenous n-3 FA within 4 wk of feeding the MO diet, and observed a significant increase in LC-HUFA and a beneficial shift in n-3:n-6 FA ratio after 8 weeks. Simple dilution accurately predicted tissue composition for most FA; however, deviations from the model were noted, suggesting selective retention of n-3, PUFA, and LC-HUFA and preferential catabolism of saturates. We conclude marine oil-based finishing diets can rapidly augment beneficial FA levels in sunshine bass fillets; however, simple dilution models do not

  1. Report of the Inter-Benchmark Protocol on New Species (Turbot and Sea bass ; IBPNew 2012). 1-5 October 2012 Copenhagen, Denmark

    OpenAIRE

    ICES

    2012-01-01

    Inter-Benchmark Workshop on NEW species (IBPNEW) met in Copenhagen (Den-mark) from 1–5 October 2012 to perform a benchmark assessment on the stocks of turbot and sea bass. All the terms of reference were addressed. In hindsight, the five days that were available for this workshop were too short. Although most work re-garding the ToRs was finished before the end of the workshop, large parts of the report still needed to be finished at the end of the meeting, and this caused consider-able delay...

  2. Ovary transcriptome profiling via artificial intelligence reveals a transcriptomic fingerprint predicting egg quality in striped bass, Morone saxatilis.

    Directory of Open Access Journals (Sweden)

    Robert W Chapman

    Full Text Available Inherited gene transcripts deposited in oocytes direct early embryonic development in all vertebrates, but transcript profiles indicative of embryo developmental competence have not previously been identified. We employed artificial intelligence to model profiles of maternal ovary gene expression and their relationship to egg quality, evaluated as production of viable mid-blastula stage embryos, in the striped bass (Morone saxatilis, a farmed species with serious egg quality problems. In models developed using artificial neural networks (ANNs and supervised machine learning, collective changes in the expression of a limited suite of genes (233 representing 90% of the eventual variance in embryo survival. Egg quality related to minor changes in gene expression (<0.2-fold, with most individual transcripts making a small contribution (<1% to the overall prediction of egg quality. These findings indicate that the predictive power of the transcriptome as regards egg quality resides not in levels of individual genes, but rather in the collective, coordinated expression of a suite of transcripts constituting a transcriptomic "fingerprint". Correlation analyses of the corresponding candidate genes indicated that dysfunction of the ubiquitin-26S proteasome, COP9 signalosome, and subsequent control of the cell cycle engenders embryonic developmental incompetence. The affected gene networks are centrally involved in regulation of early development in all vertebrates, including humans. By assessing collective levels of the relevant ovarian transcripts via ANNs we were able, for the first time in any vertebrate, to accurately predict the subsequent embryo developmental potential of eggs from individual females. Our results show that the transcriptomic fingerprint evidencing developmental dysfunction is highly predictive of, and therefore likely to regulate, egg quality, a biologically complex trait crucial to reproductive fitness.

  3. High contaminant loads in Lake Apopka's riparian wetland disrupt gene networks involved in reproduction and immune function in largemouth bass.

    Science.gov (United States)

    Martyniuk, Christopher J; Doperalski, Nicholas J; Prucha, Melinda S; Zhang, Ji-Liang; Kroll, Kevin J; Conrow, Roxanne; Barber, David S; Denslow, Nancy D

    2016-09-01

    Lake Apopka (FL, USA) has elevated levels of some organochlorine pesticides in its sediments and a portion of its watershed has been designated a US Environmental Protection Agency Superfund site. This study assessed reproductive endpoints in Florida largemouth bass (LMB) (Micropterus salmoides floridanus) after placement into experimental ponds adjacent to Lake Apopka. LMB collected from a clean reference site (DeLeon Springs) were stocked at two periods of time into ponds constructed in former farm fields on the north shore of the lake. LMB were stocked during early and late oogenesis to determine if there were different effects of contamination on LMB that may be attributed to their reproductive stage. LMB inhabiting the ponds for ~4months had anywhere from 2 to 800 times higher contaminant load for a number of organochlorine pesticides (e.g. p, p'-DDE, methoxychlor) compared to control animals. Gonadosomatic index and plasma vitellogenin were not different between reproductively-stage matched LMB collected at reference sites compared to those inhabiting the ponds. However, plasma 17β-estradiol was lower in LMB inhabiting the Apopka ponds compared to ovary stage-matched LMB from the St. Johns River, a site used as a reference site. Sub-network enrichment analysis revealed that genes related to reproduction (granulosa function, oocyte development), endocrine function (steroid metabolism, hormone biosynthesis), and immune function (T cell suppression, leukocyte accumulation) were differentially expressed in the ovaries of LMB placed into the ponds. These data suggest that (1) LMB inhabiting the Apopka ponds showed disrupted reproduction and immune responses and that (2) gene expression profiles provided site-specific information by discriminating LMB from different macro-habitats. PMID:27397556

  4. Age, growth, mortality, and reproduction of Roughtongue bass, Pronotogrammus martinicensis 9Serranidae), in the northeastern Gulf of Mexico

    Science.gov (United States)

    McBride, Richard S.; Sulak, Kenneth J.; Thurman, Paul E.; Richardson, Adam K.

    2009-01-01

    The inaccessibility of outer continental shelf reefs has made it difficult to investigate the biology of Pronotogrammus martinicensis, a small sea bass known to be numerous and widely distributed in such habitat. This study takes advantage of a series of cruises in the northeastern Gulf of Mexico that collected 1,485 individuals. Fish were collected over or in the vicinity of reef habitats with hook and line, otter trawl, and rotenone. We present a preliminary validation of an otolith ageing method and report that P. martinicensis reached a maximum size of 143 mm standard length (SL), grew to about 50% of this size within their first year, and lived to a maximum age of 15 yr. Size at age data (n = 490) fitted to the von Bertalanffy growth model yielded the predictive equation: SLt = 106.3(1 2 e [20.641{t20.646}]), where t = age in years. Gonad histology (n = 333) was examined to confirm that P. martinicensis is a protogynous, monandric hermaphrodite. We found no evidence of simultaneous hermaphroditism, which had been tentatively proposed in a previous study. Most P. martinicensis matured as females in their second year (age 1), primary oocytes developed asynchronously into secondary oocytes, and females were batch spawners. Males were postmaturational. Seminiferous tissue formed as early as age 1, but, although the rate of sex change is unknown, most fish did not function as a male until age 3 or age 4. These data provide age-based benchmarks of a common reef fish species living on the outer continental shelf of the tropical western North Atlantic Ocean.

  5. Uji Efektifitas Jamur Antagonis Trichoderma sp. Dan Gliocladium sp. Untuk Mengendalikan Penyakit Layu Fusarium

    OpenAIRE

    Nasution, Rima Yunisa

    2015-01-01

    Rima Yunisa Nasution. 2014. Effectiveness test on antagonist fungi Trichoderma sp. and Gliocladium Sp. to control fusarium disease (Fusarium oxysporum F.sp capsici) for chilli crop (Capsicum Annuum L.) at screen house. Supervised by Lahmuddin Lubis and Hassanuddin. The goal of the research is to examine antagonist fungi Trichoderma and Gliocladium virens towards F. Oxysporum that lead the fusarium disease for chilli crop at screen house. The research was conducted at Plant Dise...

  6. Tubulinosema sp. Microsporidian Myositis in Immunosuppressed Patient

    OpenAIRE

    Choudhary, Maria M.; Metcalfe, Maureen G.; Arrambide, Kathryn; Bern, Caryn; Visvesvara, Govinda S.; Pieniazek, Norman J.; Bandea, Rebecca D.; Deleon-Carnes, Marlene; Adem, Patricia; Choudhary, Moaz M.; Zaki, Sherif R.; Saeed, Musab U.

    2011-01-01

    The Phylum Microsporidia comprises >1,200 species, only 15 of which are known to infect humans, including the genera Trachipleistophora, Pleistophora, and Brachiola. We report an infection by Tubulinosema sp. in an immunosuppressed patient.

  7. SP-100 power system development status

    International Nuclear Information System (INIS)

    The SP-100 ground engineering system development project objectives, approach and status are described. The SP-100 GES development project is phase II of a three-phase program funded and directed by three United States Federal Agencies (NASA, DOD and DOE) to develop space reactor power systems for space applications in the 10 to 1000 KWe power range. The first phase of the program lasted three years, and this was completed at the end of FY 1985. SP-100 Phase I analytically and experimentally reviewed all near-term space reactor power system candidates and selected one system that best met the project mission requirements for future civilian and military space applications. The SP-100 Phase II started in fiscal year 1986 to develop the Phase I selected space reactor power system to be technically ready for space applications in the mid-to late 1990s. (author)

  8. Silencing the SpMPK1, SpMPK2, and SpMPK3 Genes in Tomato Reduces Abscisic Acid—Mediated Drought Tolerance

    Directory of Open Access Journals (Sweden)

    Yan Liang

    2013-11-01

    Full Text Available Drought is a major threat to agriculture production worldwide. Mitogen-activated protein kinases (MAPKs play a pivotal role in sensing and converting stress signals into appropriate responses so that plants can adapt and survive. To examine the function of MAPKs in the drought tolerance of tomato plants, we silenced the SpMPK1, SpMPK2, and SpMPK3 genes in wild-type plants using the virus-induced gene silencing (VIGS method. The results indicate that silencing the individual genes or co-silencing SpMPK1, SpMPK2, and SpMPK3 reduced the drought tolerance of tomato plants by varying degrees. Co-silencing SpMPK1 and SpMPK2 impaired abscisic acid (ABA-induced and hydrogen peroxide (H2O2-induced stomatal closure and enhanced ABA-induced H2O2 production. Similar results were observed when silencing SpMPK3 alone, but not when SpMPK1 and SpMPK2 were individually silenced. These data suggest that the functions of SpMPK1 and SpMPK2 are redundant, and they overlap with that of SpMPK3 in drought stress signaling pathways. In addition, we found that SpMPK3 may regulate H2O2 levels by mediating the expression of CAT1. Hence, SpMPK1, SpMPK2, and SpMPK3 may play crucial roles in enhancing tomato plants’ drought tolerance by influencing stomatal activity and H2O2 production via the ABA-H2O2 pathway.

  9. Heptaketides with antiviral activity from three endolichenic fungal strains Nigrospora sp., Alternaria sp. and Phialophora sp.

    Science.gov (United States)

    He, Jun-Wei; Chen, Guo-Dong; Gao, Hao; Yang, Fan; Li, Xiao-Xia; Peng, Tao; Guo, Liang-Dong; Yao, Xin-Sheng

    2012-09-01

    Two new heptaketides, (+)-(2S,3S,4aS)-altenuene (1a) and (-)-(2S,3S,4aR)-isoaltenuene (2a), together with six known compounds, (-)-(2R,3R,4aR)-altenuene (1b), (+)-(2R,3R,4aS)-isoaltenuene (2b), 5'-methoxy-6-methyl-biphenyl-3,4,3'-triol (3), alternariol (4), alternariol-9-methyl ether (5), and 4-hydroxyalternariol-9-methyl ether (6) were isolated from the EtOAc extract of an endolichenic fungal strain Nigrospora sphaerica (No.83-1-1-2). Compounds 1a and 1b were separated from enantiomers 1 by chiral HPLC, and so were 2a and 2b from enantiomers 2. Interestingly, 1-6 were also obtained from other two endolichenic fungal strains Alternaria alternata (No.58-8-4-1) and Phialophora sp. (No.96-1-8-1). The structures of 1-6 were elucidated by means of MS, HR-MS, NMR, and X-ray diffraction. Furthermore, the absolute configurations of 1a-2b were determined by CD experiments and CD calculation. Of these compounds, 4 and 5 showed antiviral activity against herpes simplex virus (HSV) in vitro, with IC(50) values of 13.5 and 21.3 μM, and with selective index (SI) values of 26.5 and 17.1, respectively. PMID:22613072

  10. Effects of dopaminergic system activation on feeding behavior and growth performance of the sea bass (Dicentrarchus labrax): a self-feeding approach.

    Science.gov (United States)

    Leal, Esther; Fernández-Durán, Begoña; Agulleiro, Maria Josep; Conde-Siera, Marta; Míguez, Jesús Manuel; Cerdá-Reverter, José Miguel

    2013-06-01

    Dopamine is synthesized from l-dopa and subsequently processed into norepinephrine and epinephrine. Any excess neurotransmitter can be taken up again by the neurons to be broken down enzymatically into DOPAC. The effect of dopamine on mammalian food intake is controversial. Mice unable to synthesize central dopamine die of starvation. However, studies have also shown that central injection of dopamine inhibits food intake. The effect of dopaminergic system in the fish feeding behavior has been scarcely explored. We report that the inclusion of l-dopa in the diets results in the activation of sea bass central dopaminergic system but also in the significant increase of the hypothalamic serotonin levels. Dietary l-dopa induces a decrease of food intake and feed conversion efficiency that drives a decline of all growth parameters tested. No behavioral effects were observed after l-dopa treatment. l-dopa treatment stimulated central expression of NPY and CRF. It suggests that CRF might mediate l-dopa effects on food intake but also that CRF neurons lie downstream of NPY neurons in the hierarchical forebrain system, thus controlling energy balance. Unexpectedly, dietary administration of haloperidol, a D2-receptor antagonist, cannot block dopamine effects but also induces a decline of the food intake. This decrease seems to be a side effect of haloperidol treatment since fish exhibited a decreased locomotor activity. We conclude that oral l-dopa inhibits sea bass food intake and growth. Mechanism could also involve an increase of hypothalamic serotoninergic tone. PMID:23747830

  11. The use of green pea (Pisum sativum as alternative protein source for fish meal in diets for Asian sea bass, Lates calcarifer

    Directory of Open Access Journals (Sweden)

    Erlinda S. Ganzon-Naret

    2013-07-01

    Full Text Available A 12-week feeding trial was conducted to investigate the effect of green pea P. sativum asalternative protein source for fish meal on the growth performance, feed utilization and phosphorusexcretion for Asian sea bass, Lates calcarifer. Four isonitrogenous (40% crude protein and isolipidic(10% lipid diets were prepared with the increasing substitution levels of green pea (GP for fish meal(FM at 0% (P0, 10% (P10, 20% (P20 and 30% (P30 with corresponding dietary protein levels at 0,2.6, 5.2 and 7.8% respectively in a 40% protein diet. The weight gain (WG of fish (19.69 g fed P0 diet(control without GP meal was comparable to fish fed P10 diet (17.75 g but significantly (p 0.05 werefound in the percentage of lipid among the different treatments. The peak time for phosphorus excretionwas observed after the first feeding at the end of 8 hrs. Fish fed P0 diet had the highest phosphorusexcretion which was attributed to its high level of FM component whereas fish fed P30 diet had thelowest. It appears that green pea can replace fish meal at the level of 10% in diets for sea bass withoutadverse effects on growth, feed utilization or body composition and this may also contribute toenvironmental protection as well as reduce feed cost to sustain aquaculture.

  12. Are endocrine and reproductive biomarkers altered in contaminant-exposed wild male Largemouth Bass (Micropterus salmoides) of Lake Mead, Nevada/Arizona, USA?

    Science.gov (United States)

    Goodbred, Steven L; Patiño, Reynaldo; Torres, Leticia; Echols, Kathy R; Jenkins, Jill A; Rosen, Michael R; Orsak, Erik

    2015-08-01

    Male Largemouth Bass were sampled from two locations in Lake Mead (USA), a site influenced by treated municipal wastewater effluent and urban runoff (Las Vegas Bay), and a reference site (Overton Arm). Samples were collected in summer (July '07) and spring (March '08) to assess general health, endocrine and reproductive biomarkers, and compare contaminant body burdens by analyzing 252 organic chemicals. Sperm count and motility were measured in spring. Contaminants were detected at much higher frequencies and concentrations in fish from Las Vegas Bay than Overton Arm. Those with the highest concentrations included PCBs, DDTs, PBDEs, galaxolide, and methyl triclosan. Fish from Las Vegas Bay also had higher Fulton condition factor, hepatosomatic index, and hematocrit, and lower plasma 11-ketotestosterone concentration (KT). Gonadosomatic index (GSI) and sperm motility did not differ between sites, but sperm count was lower by nearly 50% in fish from Las Vegas Bay. A positive association between KT and GSI was identified, but this association was nonlinear. On average, maximal GSI was reached at sub-maximal KT concentrations. In conclusion, the higher concentration of contaminant body burdens coupled with reduced levels of KT and sperm count in fish from Las Vegas Bay suggest that male reproductive condition was influenced by contaminant exposures. Also, the nonlinear KT-GSI association provided a framework to understand why GSI was similar between male bass from both sites despite their large difference in KT, and also suggested the existence of post-gonadal growth functions of KT at high concentrations. PMID:25733205

  13. Ecological characterization of two species of exotic fish, pumpkinseed sunfish (Lepomis gibbosus and largemouth bass (Micropterus salmoides in the international Minho river

    Directory of Open Access Journals (Sweden)

    Ana Cristina Lages

    2015-11-01

    Full Text Available The introduction of exotic species is considered the main cause for the decline of native species. The largemouth bass (Micropterus salmoides and pumpkinseed sunfish (Lepomis gibbosus are two native species from North America, introduced in Portugal to enhance sport fishing. However, their diet and great adaptability made them considered predatory and harmful. In order to understand the ecological impact of M. salmoides and L. gibbosus in the international section of the Minho River, three sampling sites were selected: two in Vila Nova de Cerveira and one in Lapela, at distance of the mouth of the river of 17 and 45 Km, respectively. The fish were gathered using fyke nets and trammel nets, electric fishing and fishing rod, with performed samplings since July 2014 until October 2015. For all fish caught the biometric data (weight, total and fork length, gonad and liver weight, sex, stomach contents analysis were registered as well as collection of otoliths and scales for age reading. Both species feed on small macroinvertebrates specially the juveniles while adults of largemouth bass and pumpkinseed sunfish prefer eat fish and gastropods, respectively. Because L. gibbosus is a recent introduction in the Minho river estuary its abundance increased a lot in the last two years and it was possible verify the increase of the fish population average length. With this work it is intended to evaluate the impact in the Minho River estuary of both exotic species studying the population structure, trophic webs and reproduction.

  14. High prevalence of buccal ulcerations in largemouth bass, Micropterus salmoides (Centrarchidae from Michigan inland lakes associated with Myzobdella lugubris Leidy 1851 (Annelida: Hirudinea

    Directory of Open Access Journals (Sweden)

    Faisal M.

    2011-02-01

    Full Text Available Widespread mouth ulcerations were observed in largemouth bass collected from eight inland lakes in the Lower Peninsula of Michigan during the summer months of 2002 and 2003. These ulcerations were associated with, and most likely caused by, leech parasitism. Through the use of morphological dichotomous keys, it was determined that all leeches collected are of one species: Myzobdella lugubris. Among the eight lakes examined, Lake Orion and Devils Lake had the highest prevalence of leech parasitism (34% and 29%, respectively and mouth ulcerations (53% and 68%, respectively. Statistical analyses demonstrated that leech and ulcer prevalence varied significantly from one lake to the other. Additionally, it was determined that the relationship between the prevalence of ulcers and the prevalence of leech attachment is significant, indicating that leech parasitism is most likely the cause of ulceration. The ulcers exhibited deep hemorrhagic centers and raised irregular edges. Affected areas lost their epithelial lining and submucosa, with masses of bacteria colonizing the damaged tissues. Since largemouth bass is a popular global sportfish and critical to the food web of inland lakes, there are concerns that the presence of leeches, damaged buccal mucosa, and general unsightliness may negatively affect this important sportfishery.

  15. Denitrification rates in marsh soils and hydrologic and water quality data for Northeast Creek and Bass Harbor Marsh watersheds, Mount Desert Island, Maine

    Science.gov (United States)

    Huntington, Thomas G.; Culbertson, Charles W.; Duff, John H.

    2011-01-01

    Nutrient enrichment from atmospheric deposition, agricultural activities, wildlife, and domestic sources is a concern at Acadia National Park because of the potential problem of water-quality degradation and eutrophication in estuaries. Water-quality degradation has been observed at the park's Bass Harbor Marsh estuary but minimal degradation is observed in Northeast Creek estuary. Previous studies at Acadia National Park have estimated nutrient inputs to estuaries from atmospheric deposition and surface-water runoff, and have identified shallow groundwater as an additional potential nutrient source. Previous studies at Acadia National Park have assumed that a certain fraction of the nitrogen input was removed through microbial denitrification, but rates of denitrification (natural or maximum potential) in marsh soils have not been determined. The U.S. Geological Survey, in cooperation with Acadia National Park, measured in situ denitrification rates in marsh soils in Northeast Creek and Bass Harbor Marsh watersheds during the summer seasons of 2008 and 2009. Denitrification was measured under ambient conditions and following inorganic nitrogen and glucose additions. Laboratory incubations of marsh soils with and without acetylene were conducted to determine average ratios of nitrous oxide (N2O) to nitrogen (N2) produced during denitrification. Surface water and groundwater samples were analyzed for nutrients, specific conductance, temperature, and dissolved oxygen. Water level was recorded continuously during the growing season in Fresh Meadow Marsh in the Northeast Creek Watershed.

  16. The influence of different dietary energy content and feeding regimes on growth and feed utilization of European sea bass (Dicentrarchus labrax, L.

    Directory of Open Access Journals (Sweden)

    Pier Paolo Gatta

    2010-01-01

    Full Text Available The growing importance of European sea bass (Dicentrarchus labrax in aquaculture underlines the need to optimize the feeding strategy for this fish species. The aim of the present study was to assess the effect of dietary energy content and feeding regime on growth performance, feed uti- lization and feeding costs for European sea bass. Seven hundreds and forthyfourfish(averageinitial forthyfourfish(averageinitial four fish ( average initial body weight 68g were randomly allocated into twelve tanks 800 l in a closed recirculation system (water temperature: 22°C; dissolved oxygen ≥90% of saturation. Three isoproteic (47% crude protein extruded diets were formulated with different lipid levels i.e. 16% (diet D16, 24% (diet D24 and 32% (diet D32 and each diet was fed at two different feeding regimes (satiation and 80% satiation accord- ing to a bifactorial experimental design. Feed intake (FI was recorded daily. After 77 days, fish were bulk weighed and growth, SGR and FCR were calculated. Feedingregimesaffectedall theanalysed Feeding regimes affected all the analysed parameters (P<0.05, whereas diet influenced only FCR, FI, protein and lipid intake and the economic efficiency ratio (EER. Fish fed the lowest energy content diet (D16 to satiation resulted in the highest feed intake, a FCR similar to that of fish fed diets D24 and D32 and in the lowest EER.

  17. Melatonin Inhibits GnRH-1, GnRH-3 and GnRH Receptor Expression in the Brain of the European Sea Bass, Dicentrarchus labrax

    Directory of Open Access Journals (Sweden)

    José Antonio Muñoz-Cueto

    2013-04-01

    Full Text Available Several evidences supported the existence of melatonin effects on reproductive system in fish. In order to investigate whether melatonin is involved in the modulation of GnRH systems in the European sea bass, we have injected melatonin (0.5 µg/g body mass in male specimens. The brain mRNA transcript levels of the three GnRH forms and the five GnRH receptors present in this species were determined by real time quantitative PCR. Our findings revealed day–night variations in the brain expression of GnRH-1, GnRH-3 and several GnRH receptors (dlGnRHR-II-1c, -2a, which exhibited higher transcript levels at mid-light compared to mid-dark phase of the photocycle. Moreover, an inhibitory effect of melatonin on the nocturnal expression of GnRH-1, GnRH-3, and GnRH receptors subtypes 1c, 2a and 2b was also demonstrated. Interestingly, the inhibitory effect of melatonin affected the expression of hypophysiotrophic GnRH forms and GnRH receptors that exhibit day–night fluctuations, suggesting that exogenous melatonin reinforce physiological mechanisms already established. These interactions between melatoninergic and GnRH systems could be mediating photoperiod effects on reproductive and other rhythmic physiological events in the European sea bass.

  18. EFFECT OF FISH-MEAL REPLACEMENT WITH POULTRY BY-PRODUCT MEAL ON THE GROWTH, TISSUE COMPOSITION AND HEMATOLOGICAL PARAMETERS OF LARGEMOUTH BASS (MICROPTERUS SALMOIDES) FED DIETS CONTAINING DIFFERENT LIPIDS

    Science.gov (United States)

    We previously showed that growth of largemouth bass (LMB) fed a diet containing different lipid (canola, chicken, or fish oil) or combination of lipids (canola + chicken oil (1:1) or menhaden fish oil + chicken oil (1:1)) was comparable to growth of LMB fed a commercial trout diet when protein in th...

  19. Regulation of endocrine and paracrine sources of insulin-like growth factors and growth hormone receptor during compensatory growth in hybrid striped bass (Morone chrysops x Morone saxatilis)

    DEFF Research Database (Denmark)

    Picha, Matthew E; Turano, Marc J; Tipsmark, Christian K; Borski, Russell J

    2008-01-01

    Compensatory growth (CG) is a period of growth acceleration that exceeds normal rates after animals are alleviated of certain growth-stunting conditions. In hybrid striped bass (HSB, Morone chrysops x M. saxatilis), 3 weeks of complete feed restriction results in a catabolic state that, when...

  20. The influence of protein and energy density in commercial diets on growth, body composition, and nutrient retention of sunshine bass Morone chrysops x Morone saxatilis reared at extreme temperature

    Science.gov (United States)

    Three growth trials were conducted with juvenile sunshine bass reared at temperatures typical of winter or summer pond culture in the Southeastern United States. The trials were designed to determine if there was an advantage to feeding a commercial high-protein/high-fat diet during winter and a lo...

  1. A comparison of two fecal collection methods for protein and amino acid digestibility coefficients for menhaden fish meal and two grades of pultry-by-product meals in market-size sunshine bass (Morone chrysops X M. saxatilis)

    Science.gov (United States)

    Apparent digestibility and availability coefficients for protein and amino acids in menhaden fish meal (MEN), pet-food grade (PBM-pet,) and feed-grade poultry by-product meal (PBM-feed) were determined for market-size (500 g) sunshine bass using passive netting or manual stripping of feces. A refer...

  2. Efficacy of florfenicol, copper sulfate and potassium permanganate in controlling a natural infection of Aeromonas hydrophila and Flavobacterium columnare in sunshine bass, Morone chrysops female x Morone saxatilis male

    Science.gov (United States)

    Sunshine bass (Morone chrysops female ' Morone saxatilis male) naturally infected with Aeromonas hydrophila and Flavobacterium columnare were randomly assigned to six treatments: 1) two treatments of waterborne exposures to copper sulfate (CuSO4), at 2.1 and at 4.2 mg/L (approximately one and two pe...

  3. Expression of surfactant proteins SP-A and SP-D in murine decidua and immunomodulatory effects on decidual macrophages.

    Science.gov (United States)

    Madhukaran, Shanmuga Priyaa; Koippallil Gopalakrishnan, Aghila Rani; Pandit, Hrishikesh; Marri, Eswari Dodagatta-; Kouser, Lubna; Jamil, Kaiser; Alhamlan, Fatimah S; Kishore, Uday; Madan, Taruna

    2016-02-01

    Surfactant proteins SP-A and SP-D are pattern recognition innate immune molecules that belong to the C-type lectin family. In lungs, they play an important role in the clearance of pathogens and control of inflammation. SP-A and SP-D are also expressed in the female reproductive tract where they play an important role in pregnancy and parturition. However, the role of SP-A and SP-D expressed at the feto-maternal interface (decidua) remains unclear. Here, we have examined the expression of SP-A and SP-D in the murine decidua at 17.5 (pre-parturition) and 19.5dpc (near parturition) and their effect on lipopolysaccharide (LPS)-treated decidual macrophages. SP-A and SP-D were localized to stromal cells in the murine decidua at 17.5 and 19.5dpc in addition to cells lining the maternal spiral artery. Purified pre-parturition decidual cells were challenged with LPS with and without SP-A or SP-D, and expression of F4/80 and TNF-α were measured by flow cytometry. On their own, SP-A or SP-D did not affect the percentage of F4/80 positive cells while they suppressed the percentage of TNF-α positive cells. However, simultaneous addition of SP-A or SP-D, together with LPS, reduced TNF-α secreting F4/80 positive cells. It is likely that exogenous administration of SP-A and SP-D in decidua can potentially control infection and inflammation mediators during spontaneous term labor and infection-induced preterm labor. Thus, the presence of SP-A and SP-D in the murine decidua is likely to play a protective role against intrauterine infection during pregnancy. PMID:26421960

  4. Metallaphotoredox-catalysed sp(3)-sp(3) cross-coupling of carboxylic acids with alkyl halides.

    Science.gov (United States)

    Johnston, Craig P; Smith, Russell T; Allmendinger, Simon; MacMillan, David W C

    2016-08-18

    In the past 50 years, cross-coupling reactions mediated by transition metals have changed the way in which complex organic molecules are synthesized. The predictable and chemoselective nature of these transformations has led to their widespread adoption across many areas of chemical research. However, the construction of a bond between two sp(3)-hybridized carbon atoms, a fundamental unit of organic chemistry, remains an important yet elusive objective for engineering cross-coupling reactions. In comparison to related procedures with sp(2)-hybridized species, the development of methods for sp(3)-sp(3) bond formation via transition metal catalysis has been hampered historically by deleterious side-reactions, such as β-hydride elimination with palladium catalysis or the reluctance of alkyl halides to undergo oxidative addition. To address this issue, nickel-catalysed cross-coupling processes can be used to form sp(3)-sp(3) bonds that utilize organometallic nucleophiles and alkyl electrophiles. In particular, the coupling of alkyl halides with pre-generated organozinc, Grignard and organoborane species has been used to furnish diverse molecular structures. However, the manipulations required to produce these activated structures is inefficient, leading to poor step- and atom-economies. Moreover, the operational difficulties associated with making and using these reactive coupling partners, and preserving them through a synthetic sequence, has hindered their widespread adoption. A generically useful sp(3)-sp(3) coupling technology that uses bench-stable, native organic functional groups, without the need for pre-functionalization or substrate derivatization, would therefore be valuable. Here we demonstrate that the synergistic merger of photoredox and nickel catalysis enables the direct formation of sp(3)-sp(3) bonds using only simple carboxylic acids and alkyl halides as the nucleophilic and electrophilic coupling partners, respectively. This metallaphotoredox

  5. Evaluation of nutrient components of Bangia sp.

    Institute of Scientific and Technical Information of China (English)

    李水军; 马家海; 纪焕红; 谢恩义

    2003-01-01

    General nutrient components including moisture, ash, crude protein, lipid and carbohy-drate, inorganic elements, photosynthetic pigments, amino acids and fatty acids of Bangia sp. Were an-alyzed and compared with Porphyra yezoensis. The results indicated that the content of protein, totalamino acids and free amino acids accounted for 47.2 %, 42.6 % and 3.3 % in dried Bangia sp. , respec-tively, all essential amino acids were present in relatively high levels and desirable composition. Lipidwas 1.8 %, in which the eicosapentaenoic acid (EPA) reached 50.5 %. In per 100 g dried material, thecontent of chlorophyll, carotenoids, phycoerythrin and phycocyanin accounted for 766, 208, 4 600 and2 963 mg, respectively. Inorganic elements necessary for human body were found to be abundant inBangia sp. The results showed that the nutritive value of Bangia sp. Was the highest among algae re-ported so far, and the industry of Bangia sp. Was provided with significant economic importance and de-veloping potential.

  6. Interaction of uranium with Pleurotus sp

    International Nuclear Information System (INIS)

    Uptake of uranium by higher fungi, such as mushroom is little elucidated. We have studied the interaction of uranium with Pleurotus sp. (a mushroom) in pure culture over a wide range of U concentration (50-3000 mg/L). The Pleurotus sp. was cultured in two different media. One was rice bran medium, and the other was agar (yeast extract, peptone and dextrose) medium. The uptake of uranium in Pleurotus sp. was examined by alpha ray autoradiography (A,A), X-ray fluorescence spectroscopy (XRF) and scanning microcopy (SEM) equipped with EDS. In the agar medium, the higher uranium concentration gave lower growth of mycelia, and no fruiting body was observed. In the rice bran medium, the fruiting body was grown at U concentrations up to 1000 mg/L. The AA and XRF analysis showed that uranium taken up in the fruiting body was below the detection limit. The SEM-EDS analysis indicated that U was distributed in the limited region and was not transported to the mycelia far from U containing medium. It is concluded that uranium affects the growth of Pleurotus sp., and little uranium is taken up by Pleurotus sp. during the growth of both mycelia and fruiting body. (author)

  7. Bioaccumulation of Total and Methyl Mercury in Three Earthworm Species (Drawida sp., Allolobophora sp., and Limnodrilus sp.)

    OpenAIRE

    Zhang, Zhong Sheng; Zheng, Dong Mei; Wang, Qi Chao; Lv, Xian Guo

    2009-01-01

    We determined total and methyl mercury contents in soil, three earthworm species and their vomitus to study the species-specific differences of mercury bioconcentration in Huludao City, a heavily polluted region by chlor-alkali and nonferrous metal smelting industry in Liaoning Province, northeast China. Total and methyl mercury contents were 7.20 mg/kg and 6.94 ng/g in soil, 1.43 mg/kg and 43.03 ng/g in Drawida sp., 2.80 mg/kg and 336.52 ng/g in Alolobophora sp., respectively. Total mercury ...

  8. A digital terrain model of bathymetry and shallow-zone bottom-substrate classification for Spednic Lake and estimates of lake-level-dependent habitat to support smallmouth bass persistence modeling

    Science.gov (United States)

    Dudley, Robert W.; Schalk, Charles W.; Stasulis, Nicholas W.; Trial, Joan G.

    2011-01-01

    In 2009, the U.S. Geological Survey entered into a cooperative agreement with the International Joint Commission, St. Croix River Board to do an analysis of historical smallmouth bass habitat as a function of lake level for Spednic Lake in an effort to quantify the effects, if any, of historical lake-level management and meteorological conditions (from 1970 to 2009) on smallmouth bass year-class failure. The analysis requires estimating habitat availability as a function of lake level during spawning periods from 1970 to 2009, which is documented in this report. Field work was done from October 19 to 23, and from November 2 to 10, 2009, to acquire acoustic bathymetric (depth) data and acoustic data indicating the character of the surficial lake-bottom sediments. Historical lake-level data during smallmouth bass spawning (May-June) were applied to the bathymetric and surficial-sediment type data sets to produce annual historic estimates of smallmouth-bass-spawning-habitat area. Results show that minimum lake level during the spawning period explained most of the variability (R2 = 0.89) in available spawning habitat for nearshore areas of shallow slope (less than 10 degrees) on the basis of linear correlation. The change in lake level during the spawning period explained most of the variability (R2 = 0.90) in available spawning habitat for areas of steeper slopes (10 to 40 degrees) on the basis of linear correlation. The next step in modeling historic smallmouth bass year-class persistence is to combine this analysis of the effects of lake-level management on habitat availability with meteorological conditions.

  9. Surfactant Proteins SP-A and SP-D Modulate Uterine Contractile Events in ULTR Myometrial Cell Line.

    Directory of Open Access Journals (Sweden)

    Georgios Sotiriadis

    Full Text Available Pulmonary surfactant proteins SP-A and SP-D are pattern recognition innate immune molecules. However, there is extrapulmonary existence, especially in the amniotic fluid and at the feto-maternal interface. There is sufficient evidence to suggest that SP-A and SP-D are involved in the initiation of labour. This is of great importance given that preterm birth is associated with increased mortality and morbidity. In this study, we investigated the effects of recombinant forms of SP-A and SP-D (rhSP-A and rhSP-D, the comprising of trimeric lectin domain on contractile events in vitro, using a human myometrial cell line (ULTR as an experimental model. Treatment with rhSP-A or rhSP-D increased the cell velocity, distance travelled and displacement by ULTR cells. rhSP-A and rhSP-D also affected the contractile response of ULTRs when grown on collagen matrices showing reduced surface area. We investigated this effect further by measuring contractility-associated protein (CAP genes. Treatment with rhSP-A and rhSP-D induced expression of oxytocin receptor (OXTR and connexin 43 (CX43. In addition, rhSP-A and rhSP-D were able to induce secretion of GROα and IL-8. rhSP-D also induced the expression of IL-6 and IL-6 Ra. We provide evidence that SP-A and SP-D play a key role in modulating events prior to labour by reconditioning the human myometrium and in inducing CAP genes and pro-inflammatory cytokines thus shifting the uterus from a quiescent state to a contractile one.

  10. Sp(3, R) coadjoint orbit theory

    International Nuclear Information System (INIS)

    The algebraic mean field method is applied to the symplectic Lie algebra sp(3, R) that describes geometrical collective states in atomic nuclei. The expectations of sp(3, R) generators define a symplectic density matrix. The mean field approximation restricts the densities to a manifold that is a coadjoint orbit of the transformation group Sp(3, R) and a level surface of the symplectic Casimir functions. Compared to representation theory, mean field theory is technically simpler, but yields similar predictions for physical properties of collective states. The critical points of the energy functional on a coadjoint orbit surface define rotational bands. The deformation, kinetic energy and Kelvin circulation of principal axis symplectic rotors are determined as a function of the angular momentum. Illustrative applications of coadjoint orbit theory are reported for the yrast rotational bands of a light 20Ne and a heavy 166Er deformed isotope. (author)

  11. SP2Bench: A SPARQL Performance Benchmark

    CERN Document Server

    Schmidt, Michael; Lausen, Georg; Pinkel, Christoph

    2008-01-01

    Recently, the SPARQL query language for RDF has reached the W3C recommendation status. In response to this emerging standard, the database community is currently exploring efficient storage techniques for RDF data and evaluation strategies for SPARQL queries. A meaningful analysis and comparison of these approaches necessitates a comprehensive and universal benchmark platform. To this end, we have developed SP$^2$Bench, a publicly available, language-specific SPARQL performance benchmark. SP$^2$Bench is settled in the DBLP scenario and comprises both a data generator for creating arbitrarily large DBLP-like documents and a set of carefully designed benchmark queries. The generated documents mirror key characteristics and social-world distributions encountered in the original DBLP data set, while the queries implement meaningful requests on top of this data, covering a variety of SPARQL operator constellations and RDF access patterns. As a proof of concept, we apply SP$^2$Bench to existing engines and discuss ...

  12. BUSCANDO UM BIOHERBICIDA CONTRA Cyperus sp (tiririca

    Directory of Open Access Journals (Sweden)

    Cristine Benfatti Gonzalez

    2001-01-01

    Full Text Available As an alternative to conventional control of Cyperus sp (herbaceousnutsedge, with chemical products, it rises the biological control, making use of phytopathogenic microorganisms. As results of the whole production of Cercospora henningsii e Cercospora caricis by fermentation in potato-dextrose (PD liquid medium, it was observed that both species obtained the optimum growth at 36o C. C. henningsii attained the best value after a period of 72 h of fermentation and C. caricis attained it after 60 h. The field experiments, against Cyperus sp (nutsedge, with the obtained products by liquid fermentation of Cercospora sp (C. henningsii and/or C. caribaea, andwith the use of "wet chamber", proved the pathogenic potential bioherbicide of these microorganisms. = Como alternativa ao controle convencional do Cyperus sp (tiririca usando produtos químicos, surge o controle biológico, empregando-se microrganismos fitopatógenos. Como resultados da produção massal de Cercospora henningsii eCercospora caricis através de cultivo submerso em meio de cultura batata-dextrose (BD, observou-se que ambas espécies obtiveram um crescimento ótimo a 36o C, sendo que para C. henningsii se deu com 72 h de fermentação e para C. caricis com 60 h. Os experimentos de campo, bem como os de Câmara úmida, contra Cyperus sp (tiririca,com os produtos obtidos pelo cultivo submerso de Cercospora sp (C. henningsii e/ou C. caribaea, demonstraram a patogenicidade, ou seja, o potencial bioherbicida desses microrganismos.

  13. Analysis list: Sp1 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Sp1 Placenta,Pluripotent stem cell + mm9 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/target/Sp1....1.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/target/Sp1.5.tsv http://dbarchive.biosc...iencedbc.jp/kyushu-u/mm9/target/Sp1.10.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/colo/Sp1.Placenta....tsv,http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/colo/Sp1.Pluripotent_stem_cell

  14. Blastocystis sp. Infection Mimicking Clostridium Difficile Colitis

    Science.gov (United States)

    Gil, Gaby S.; Chaudhari, Shobhana; Shady, Ahmed; Caballes, Ana; Hong, Joe

    2016-01-01

    We report an unusual case of severe diarrhea related to Blastocystis sp. infection in a patient with end stage renal disease on hemodialysis. The patient was admitted due to profuse diarrhea associated with fever and leukocytosis. Pertinent stool work-up such as leukocytes in stool, stool culture, clostridium difficile toxin B PCR, and serology for hepatitis A, hepatitis B, and hepatitis C and cytomegalovirus screening were all negative. Ova and parasite stool examination revealed Blastocystis sp. The patient was given intravenous metronidazole with clinical improvement by day three and total resolution of symptoms by day ten. PMID:27247810

  15. The SP theory of intelligence: an overview

    OpenAIRE

    J. Gerard Wolff

    2013-01-01

    This article is an overview of the SP theory of intelligence, which aims to simplify and integrate concepts across artificial intelligence, mainstream computing and human perception and cognition, with information compression as a unifying theme. It is conceived of as a brain-like system that receives "New" information and stores some or all of it in compressed form as "Old" information; and it is realised in the form of a computer model, a first version of the SP machine. The matching and un...

  16. SP-100 converter multicouple thermoelectric cell

    International Nuclear Information System (INIS)

    The General Electric Company is under contract to DOE to design, fabricate, and test an SP-100 Ground Engineering System. This paper provides a description of the SP-100 space reactor power system configuration, and a more detailed description of the power conversion subsystem (PCSS) and the key building block of the power converter, the thermoelectric cell. The functions of the various elements of the PCSS and the cells are also presented. These cells convert the thermal energy from the reactor into electrical power at the desired voltage while being conductively coupled to the hot and cold side heat exchangers to maximize the power output and system specific power

  17. Ocorrência de Giardia sp. e Cryptosporidium sp. em Leopardus weidii de vida livre Occurrence of Giardia sp. and Cryptosporidium sp. in Leopardus weidii of wild life

    OpenAIRE

    Camila Belmonte Oliveira; João Fábio Soares; Aleksandro Schafer da Silva; Marcos Kipper da Silva; Edson Luis Salomão; Silvia Gonzalez Monteiro

    2008-01-01

    Leopardus weiidi (gato-maracajá) é um mamífero neotropical de ampla distribuição no Brasil. No município de Cachoeira do Sul, RS, um exemplar foi capturado para tratamento, quando se coletou uma amostra de fezes, que foi analisada pelo método de centrífugo-flutuação com sulfato de zinco para pesquisa de parasitos. Foi observada elevada infecção por cistos de Giardia sp. e oocistos de Cryptosporidium sp.. Com base nos resultados, conclui-se que L. weeidi é hospedeiro desses protozoários.Leopar...

  18. Dietary Mannan Oligosaccharides: Counteracting the Side Effects of Soybean Meal Oil Inclusion on European Sea Bass (Dicentrarchus labrax) Gut Health and Skin Mucosa Mucus Production?

    Science.gov (United States)

    Torrecillas, Silvia; Montero, Daniel; Caballero, Maria José; Pittman, Karin A.; Custódio, Marco; Campo, Aurora; Sweetman, John; Izquierdo, Marisol

    2015-01-01

    The main objective of this study was to assess the effects of 4 g kg−1 dietary mannan oligosaccharides (MOS) inclusion in soybean oil (SBO)- and fish oil (FO)-based diets on the gut health and skin mucosa mucus production of European sea bass juveniles after 8 weeks of feeding. Dietary MOS, regardless of the oil source, promoted growth. The intestinal somatic index was not affected, however dietary SBO reduced the intestinal fold length, while dietary MOS increased it. The dietary oil source fed produced changes on the posterior intestine fatty acid profiles irrespective of MOS dietary supplementation. SBO down-regulated the gene expression of TCRβ, COX2, IL-1β, TNFα, IL-8, IL-6, IL-10, TGFβ, and Ig and up-regulated MHCII. MOS supplementation up-regulated the expression of MHCI, CD4, COX2, TNFα, and Ig when included in FO-based diets. However, there was a minor up-regulating effect on these genes when MOS was supplemented in the SBO-based diet. Both dietary oil sources and MOS affected mean mucous cell areas within the posterior gut, however the addition of MOS to a SBO diet increased the mucous cell size over the values shown in FO fed fish. Dietary SBO also trends to reduce mucous cell density in the anterior gut relative to FO, suggesting a lower overall mucosal secretion. There are no effects of dietary oil or MOS in the skin mucosal patterns. Complete replacement of FO by SBO, modified the gut fatty acid profile, altered posterior gut-associated immune system (GALT)-related gene expression and gut mucous cells patterns, induced shorter intestinal folds and tended to reduce European sea bass growth. However, when combined with MOS, the harmful effects of SBO appear to be partially balanced by moderating the down-regulation of certain GALT-related genes involved in the functioning of gut mucous barrier and increasing posterior gut mucous cell diffusion rates, thus helping to preserve immune homeostasis. This denotes the importance of a balanced

  19. CULTIVO EXPERIMENTAL DEL CLADOCERO Moina sp ALIMENTADO CON Ankistrodesmus sp y Saccharomyces cereviseae

    OpenAIRE

    Martha Prieto; Litia De la Cruz; Mario Morales

    2006-01-01

    Objetivo. Realizar un cultivo experimental de cladócero Moina sp alimentado con Ankistrodesmus sp y Saccharomyces cereviseae. Materiales y métodos. Fueron realizados cultivos experimentales de la cepa de cladóceros Moina sp en el laboratorio de Alimento Vivo de la Universidad de Córdoba, bajo condiciones controladas de temperatura (22oC), pH (7.6), intensidad lumínica (2000 lux) y aireación. Se emplearon dos dietas de alimento como tratamiento (Ankistrodesmus y Ankistrodesmus mas Saccharomyce...

  20. Assessment of Surfactant Protein A (SP-A dependent agglutination

    Directory of Open Access Journals (Sweden)

    Griese Matthias

    2010-11-01

    Full Text Available Abstract Background Monomers of the collectin surfactant associated protein-A (SP-A are arranged in trimers and higher oligomers. The state of oligomerization differs between individuals and likely affects SP-A's functional properties. SP-A can form aggregates together with other SP-A molecules. Here we report and assess a test system for the aggregate forming properties of SP-A in serum and broncho-alveolar lavage samples. Methods Anti-SP-A antibodies fixed to latex beads bound SP-A at its N-terminal end and allowed the interaction with other SP-A molecules in a given sample by their C-terminal carbohydrate recognition domain (CRD to agglutinate the beads to aggregates, which were quantified by light microscopy. Results SP-A aggregation was dependent on its concentration, the presence of calcium, and was dose-dependently inhibited by mannose. Unaffected by the presence of SP-D no aggregation was observed in absence of SP-A. The more complex the oligomeric structure of SP-A present in a particular sample, the better was its capability to induce aggregation at a given total concentration of SP-A. SP-A in serum agglutinated independently of the pulmonary disease; in contrast SP-A in lung lavage fluid was clearly inferior in patients with chronic bronchitis and particularly with cystic fibrosis compared to controls. Conclusions The functional status of SP-A with respect to its aggregating properties in serum and lavage samples can be easily assessed. SP-A in lung lavage fluid in patients with severe neutrophilic bronchitis was inferior.

  1. Partial pressure (or fugacity) of carbon dioxide, salinity and other variables collected from Surface underway observations using Barometric pressure sensor, Carbon dioxide (CO2) gas analyzer and other instruments from Trans Future 5 in the Bass Strait, Coral Sea and others from 2006-06-27 to 2006-12-09 (NCEI Accession 0144357)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0144357 includes Surface underway data collected from Trans Future 5 in the Bass Strait, Coral Sea, North Pacific Ocean, Philippine Sea, Solomon Sea,...

  2. Partial pressure (or fugacity) of carbon dioxide, salinity and other variables collected from underway - surface observations using Carbon dioxide (CO2) gas analyzer, Shower head chamber equilibrator for autonomous carbon dioxide (CO2) measurement and other instruments from the SOUTHERN SURVEYOR in the Bass Strait, Coral Sea and others from 2008011 to 2010-10-31 (NODC Accession 0115181)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0115181 includes chemical, meteorological, physical and underway - surface data collected from SOUTHERN SURVEYOR in the Bass Strait, Coral Sea, Great...

  3. Partial pressure (or fugacity) of carbon dioxide, salinity and other variables collected from underway - surface observations using Carbon dioxide (CO2) gas analyzer, Shower head chamber equilibrator for autonomous carbon dioxide (CO2) measurement and other instruments from the Natalie Schulte in the Bass Strait, North Pacific Ocean and others from 2010-10-01 to 2012-06-21 (NODC Accession 0108233)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0108233 includes chemical, meteorological, physical and underway - surface data collected from Natalie Schulte in the Bass Strait, North Pacific...

  4. Partial pressure (or fugacity) of carbon dioxide, salinity and other variables collected from underway - surface observations using Carbon dioxide (CO2) gas analyzer, Shower head chamber equilibrator for autonomous carbon dioxide (CO2) measurement and other instruments from the Trans Future 5 in the Bass Strait, Coral Sea and others from 2006-06-27 to 2011-08-05 (NODC Accession 0081048)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0081048 includes chemical, meteorological, physical and underway - surface data collected from Trans Future 5 in the Bass Strait, Coral Sea, Inland...

  5. Partial pressure (or fugacity) of carbon dioxide, salinity and other variables collected from Surface underway observations using Barometric pressure sensor, Carbon dioxide (CO2) gas analyzer and other instruments from Trans Future 5 in the Bass Strait, Coral Sea and others from 2012-01-16 to 2012-12-07 (NCEI Accession 0144347)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0144347 includes Surface underway data collected from Trans Future 5 in the Bass Strait, Coral Sea, East China Sea (Tung Hai), Inland Sea (Seto...

  6. Ovos de Toxocara sp. e larvas de Ancylostoma sp. em praça pública de Lavras, MG Toxocara sp. eggs and Ancylostoma sp. larva in public parks, Brazil

    Directory of Open Access Journals (Sweden)

    Antônio Marcos Guimarães

    2005-04-01

    Full Text Available Larva migrans visceral e cutânea são zoonoses parasitárias causadas pela infecção da larva de Toxocara sp. e Ancylostoma sp., respectivamente. O objetivo do estudo foi verificar a contaminação por ovos de Toxocara sp. e ovos e larvas de Ancylostoma sp. em amostras de solos coletadas de praças públicas e de áreas de recreação infantil de Lavras, Estado de Minas Gerais, por meio da técnica de centrífugo-flutuação e do método de Baermann. A ocorrência de ovos de Toxocara sp. e, ovos e larvas de Ancylostoma sp. foi observada em 69,6% (16/23 das amostras de solo coletadas de praças públicas. A contaminação somente por ovos de Ancylostoma sp. em amostras de solo coletadas em escolas/creches foi de 22,2% (4/18. A percentagem de amostras de areia coletadas de escolas/creches contaminadas somente com larvas de Ancylostoma sp. foi de 11,1% (2/18. Praças públicas são as áreas com maior risco potencial de infecção por Toxocara sp. e Ancylostoma sp. Exame coproparasitológico realizado em 174 amostras de fezes de cães observou 58% e 23%, respectivamente, com ovos de Ancylostoma sp. e Toxocara sp.Visceral and cutaneous larva migrans are parasitic zoonoses caused by the infection of larval nematodes Toxocara sp. and Ancylostoma sp. respectively. The objective of this study was to investigate the contamination by Toxocara sp. eggs and Ancylostoma sp. eggs and larva of soil samples collected from public parks and children's playground areas in state of Minas Gerais, Brazil, using both Baermann's method and centrifugal flotation technique. Toxocara sp. and Ancylostoma sp. eggs were observed in soil samples collected from public squares in 17.4% (4/23 and 69.6 (16/23 respectively. In schools and child day care settings the contamination by Ancylostoma sp. larva in sand samples was 11.1% (2/18. Public parks are settings of more potential risk of Toxocara sp. eggs and Ancylostoma sp. infection. Stool parasitology testing of 174 stool

  7. Framework, The Physics of sp(2,R)

    CERN Document Server

    Hawthorn, Ian; Ussher, Matthew

    2015-01-01

    A mathematical framework for relativistic quantum field theory is constructed with natural symmetry $\\mathsf{so}(2,3)= \\mathsf{sp}(2,\\mathbb{ R})$. In this framework gravity and electromagnetism unify as aspects of the geometry. The source equation for gravity differs from Einstein's equation and permits behaviour that could explain dark matter.

  8. Biosorption of uranium by Azolla, SP, Brazil

    Energy Technology Data Exchange (ETDEWEB)

    Vieira, Ludmila C.; Alves, Eliakim G.; Marumo, Julio T., E-mail: lcvieira@ipen.br [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil); Ferreira, Rafael V. de P., E-mail: rafael@itatijuca.com [Itatijuca Biotech, Sao Paulo, SP (Brazil); Canevesi, Rafael L.S.; Silva, Edson A., E-mail: edson.silva2@unioeste.br [Universidade Estadual do Oeste Parana (UNIOESTE), Toledo, PR (Brazil)

    2015-07-01

    Radioactive liquid waste needs special attention and requires suitable treatment before deposition. Among the potential technologies under development for the treatment of liquid radioactive wastes the biosorption has been highlighted by being an efficient and low cost technique. Biosorption process involves the exchange of ions contained in the biomass matrix by others present in solution. There are many biomasses that could be applied in treatment of radioactive wastes, for example, agricultural residues and macrophyte. The aim of this study is evaluate the ability of the Azolla sp., a floating aquatic plant, to absorb uranium in solution. Azolla sp. is a macrophyte that has been used to treat effluents containing heavy metals. The biosorption capacity of uranium by Azolla sp. was experimentally determined and modeled by isotherms. Experiments were performed to determine metal uptake, and then the solutions were analyzed by inductively coupled plasma optical emission spectrometry (ICP-OES). The isotherms applied to model the data was Langmuir, Freundlich, Sips Toth, Redlich Peternson, Two-Site-Langmuir, Radke Prausnitz to develop a technique for the treatment of radioactive liquid waste generated at the Nuclear and Energy Research Institute (IPEN-CNEN/SP), Brazil. (author)

  9. Ocorrência de Giardia sp. e Cryptosporidium sp. em Leopardus weidii de vida livre Occurrence of Giardia sp. and Cryptosporidium sp. in Leopardus weidii of wild life

    Directory of Open Access Journals (Sweden)

    Camila Belmonte Oliveira

    2008-04-01

    Full Text Available Leopardus weiidi (gato-maracajá é um mamífero neotropical de ampla distribuição no Brasil. No município de Cachoeira do Sul, RS, um exemplar foi capturado para tratamento, quando se coletou uma amostra de fezes, que foi analisada pelo método de centrífugo-flutuação com sulfato de zinco para pesquisa de parasitos. Foi observada elevada infecção por cistos de Giardia sp. e oocistos de Cryptosporidium sp.. Com base nos resultados, conclui-se que L. weeidi é hospedeiro desses protozoários.Leopardus weiidi (gato-maracajá is a neo-tropical mammal with has a wide distribution in Brazil. In the municipality of Cachoeira do Sul, RS, a fecal sample from a specimen captured for treatment was collected and analyzed by the centrifugal-flotation method with zinc sulfate for research of parasites. A large number of cysts of Giardia sp. and oocysts of Cryptosporidium sp. was observed. The results indicate that L. weeidi is a host of these protozoa.

  10. A new virginae butanolide from Streptomyces sp.

    Institute of Scientific and Technical Information of China (English)

    Xiang LI; Yi Nan ZHENG; Wen Han LIN; Isabel SATTLER

    2006-01-01

    A novel butanolide, named virginaebutanolide F (1), was isolated from the lyophilized culture broth of Streptomyces sp., along with a known compound virginaebutanolide C (2). Their structures including the stereochemistry were elucidated on the basis of extensive 1D and 2D NMR as well as HRESI-MS and CD spectroscopic analysis.

  11. Biosorption of uranium by Azolla, SP, Brazil

    International Nuclear Information System (INIS)

    Radioactive liquid waste needs special attention and requires suitable treatment before deposition. Among the potential technologies under development for the treatment of liquid radioactive wastes the biosorption has been highlighted by being an efficient and low cost technique. Biosorption process involves the exchange of ions contained in the biomass matrix by others present in solution. There are many biomasses that could be applied in treatment of radioactive wastes, for example, agricultural residues and macrophyte. The aim of this study is evaluate the ability of the Azolla sp., a floating aquatic plant, to absorb uranium in solution. Azolla sp. is a macrophyte that has been used to treat effluents containing heavy metals. The biosorption capacity of uranium by Azolla sp. was experimentally determined and modeled by isotherms. Experiments were performed to determine metal uptake, and then the solutions were analyzed by inductively coupled plasma optical emission spectrometry (ICP-OES). The isotherms applied to model the data was Langmuir, Freundlich, Sips Toth, Redlich Peternson, Two-Site-Langmuir, Radke Prausnitz to develop a technique for the treatment of radioactive liquid waste generated at the Nuclear and Energy Research Institute (IPEN-CNEN/SP), Brazil. (author)

  12. Carbon sp chains in graphene nanoholes

    DEFF Research Database (Denmark)

    Castelli, Ivano Eligio; Ferri, Nicola; Onida, Giovanni;

    2012-01-01

    Nowadays sp carbon chains terminated by graphene or graphitic-like carbon are synthesized routinely in several nanotech labs. We propose an ab initio study of such carbon-only materials, by computing their structure and stability, as well as their electronic, vibrational and magnetic properties. We...

  13. Ragweed (Ambrosia sp. seeds in bird feed

    Directory of Open Access Journals (Sweden)

    Frick, G.

    2011-01-01

    Full Text Available In 2005, the Swiss official feed inspection of Agroscope Liebefeld-Posieux Research Station (ALP was mandated to check bird feed and raw materials for the presence of Ambrosia sp. seeds. Indeed, such seeds were found in varying amounts when analyzed in the feed microscopy laboratory. The producers were informed, and a limit of intervention (50 mg.kg-1 ≈ 9 to 10 seeds.kg-1 was finally set for this undesirable component. The results of five years of controls show, at first, around 50% of contaminated samples. With appropriate measures, the level of contamination could be lowered in the following years. In parallel, the size of Ambrosia sp. seeds and the sieves to be used for routine analyses were checked. Ambrosia sp. seeds found in feed checked in Switzerland were seldom larger ("wider" than 3.5 mm and never smaller than 1.5 mm. Several EU Member States started their own control and monitoring programs. The results of studies from Germany, Slovenia and Denmark, presented by their feed microscopists at the IAG meetings, indicate the presence of Ambrosia sp. seeds in 21 to 75% of the products put on the market.

  14. Surfactant proteins, SP-A and SP-D, in respiratory fungal infections: their role in the inflammatory response.

    Science.gov (United States)

    Carreto-Binaghi, Laura Elena; Aliouat, El Moukhtar; Taylor, Maria Lucia

    2016-01-01

    Pulmonary surfactant is a complex fluid that comprises phospholipids and four proteins (SP-A, SP-B, SP-C, and SP-D) with different biological functions. SP-B, SP-C, and SP-D are essential for the lungs' surface tension function and for the organization, stability and metabolism of lung parenchyma. SP-A and SP-D, which are also known as pulmonary collectins, have an important function in the host's lung immune response; they act as opsonins for different pathogens via a C-terminal carbohydrate recognition domain and enhance the attachment to phagocytic cells or show their own microbicidal activity by increasing the cellular membrane permeability. Interactions between the pulmonary collectins and bacteria or viruses have been extensively studied, but this is not the same for fungal pathogens. SP-A and SP-D bind glucan and mannose residues from fungal cell wall, but there is still a lack of information on their binding to other fungal carbohydrate residues. In addition, both their relation with immune cells for the clearance of these pathogens and the role of surfactant proteins' regulation during respiratory fungal infections remain unknown. Here we highlight the relevant findings associated with SP-A and SP-D in those respiratory mycoses where the fungal infective propagules reach the lungs by the airways. PMID:27250970

  15. Production vivrière et accès à la terre dans un village de basse Côte d'Ivoire

    OpenAIRE

    Biarnès, Anne; Colin, Jean-Philippe

    1987-01-01

    Cet article présente les systèmes de culture vivriers pratiqués dans un village en basse Côte d'Ivoire et quelques-uns de leurs déterminants. Sont d'abord évoquées les modalités de l'insertion spatiale des cultures vivrières dans l'agriculture de plantation; puis, après la présentation d'une typologie des systèmes de culture vivriers, sont analysées la fonction économique des principales cultures (autoconsommation ou vente) et l'incidence des contraintes culturales et foncières sur la product...

  16. Implementation of a non-lethal biopsy punch monitoring program for mercury in smallmouth bass, Micropterus dolomieu Lacepède, from the Eleven Point River, Missouri

    Science.gov (United States)

    Ackerson, J.R.; Schmitt, C. J.; McKee, M.J.; Brumbaugh, W.G.

    2013-01-01

    A non-lethal biopsy method for monitoring mercury (Hg) concentrations in smallmouth bass (Micropterus dolomieu; smallmouth) from the Eleven Point River in southern Missouri USA was evaluated. A biopsy punch was used to remove a muscle tissue plug from the area immediately below the anterior dorsal fin of 31 smallmouth. An additional 35 smallmouth (controls) were held identically except that no tissue plug was removed. After sampling, all fish were held in a concrete hatchery raceway for 6 weeks. Mean survival at the end of the holding period was 97 % for both groups. Smallmouth length, weight and Fulton’s condition factor at the end of the holding period were also similar between plugged and non-plugged controls, indicating that the biopsy procedure had minimal impact on growth under these conditions. Tissue plug Hg concentrations were similar to smallmouth Hg data obtained in previous years by removing the entire fillet for analysis.

  17. Growth and nitrogen metabolism of sea bass fed graded levels of nucleic acid nitrogen from yeast or RNA extract as partial substitute for protein nitrogen from fish meal

    Directory of Open Access Journals (Sweden)

    S. Kaushik

    2010-01-01

    Full Text Available Some studies carried out in mammalian models have shown de novo synthesis and salvage of nucleotides to be a costly metabolic process and a dietary supplementation with nucleic acids (NA or nucleotides has been suggested to result in a protein sparing action (Sanderson and He, 1994. On the other hand, high levels of dietary NA could have toxic effects and lead to disturbance in protein, lipid and carbohydrate metabolism in monogastric animals lacking uricase activity, an enzyme involved in NA degradation (Clifford and Story, 1976. So far, there is no clear indication of such effects in fish fed nucleic acid-enriched diets (Tacon and Cooke, 1980; Rumsey et al., 1992; Fournier et al., 2002. The aim of this experiment was to investigate growth response and N metabolism in juvenile sea bass (D. labrax fed diets supplying graded levels of nucleic acid N from dry brewer's yeast or RNA extract as partial substitutes for protein nitrogen provided by fish meal.

  18. Molecular Characterization and Functional Regulation of Melanocortin 2 Receptor (MC2R) in the Sea Bass. A Putative Role in the Adaptation to Stress

    Science.gov (United States)

    Agulleiro, Maria Josep; Sánchez, Elisa; Leal, Esther; Cortés, Raúl; Fernández-Durán, Begoña; Guillot, Raúl; Davis, Perry; Dores, Robert M.; Gallo-Payet, Nicole; Cerdá-Reverter, José Miguel

    2013-01-01

    The activation of melanocortin 2 receptor (MC2R) by ACTH mediates the signaling cascade leading to steroid synthesis in the interrenal tissue (analogous to the adrenal cortex in mammals) of fish. However, little is known about the functional regulation of this receptor in fish. In this work described, we cloned sea bass MC2R from a liver cDNA. SbMC2R requires the melanocortin 2 receptor accessory protein (MRAP) for its functional expression. Dietary cortisol but not long-term stress protocols downregulated interrenal sbMC2R expression. Data suggest the existence of a negative feedback on interrenal sbMC2R expression imposed by local or systemic glucocorticoids. This feedback could be involved in long-term stress adaptation by regulating interrenal sensitivity to ACTH. ACTH-induced MC2R activation stimulates hepatic lipolysis, suggesting that ACTH may mediate stress-induced effects upstream of cortisol release. PMID:23724142

  19. Differential regulation of cystic fibrosis transmembrane conductance regulator and Na+,K+ -ATPase in gills of striped bass, Morone saxatilis: effect of salinity and hormones

    DEFF Research Database (Denmark)

    Madsen, Steffen; Jensen, Lars Nørholm; Tipsmark, Christian Kølbaek;

    2007-01-01

    Effects of salinity and hormones on cystic fibrosis transmembrane conductance regulator (CFTR) and alpha-subunit Na(+),K(+) -ATPase (alpha-NKA) mRNA (analysed by semi-quantitative PCR) and protein expression (analysed by western blotting and immunocytochemistry) were investigated in gills of...... striped bass. Freshwater (FW) to seawater (SW) transfer induced a disturbance in serum [Na(+)]. Gill CFTR protein, mRNA level and Na(+),K(+) -ATPase activity were unaffected by SW transfer, whereas alpha-NKA mRNA increased after transfer. CFTR immunoreactivity was observed in large cells in FW and SW gill...... filaments at equal intensity. Cortisol decreased serum [Na(+)] in FW fish, but had no effect on gill Na(+),K(+) -ATPase activity, alpha-NKA and CFTR mRNA levels. Incubation of gill tissue with cortisol (24 h, >0.01 micro g/ml) and epidermal growth factor (EGF 10 micro g/ml) decreased CFTR mRNA levels...

  20. Analysis of EST database for two subspecies of largemouth bass%大口黑鲈两个亚种EST数据库分析

    Institute of Scientific and Technical Information of China (English)

    景燕娟; 白俊杰; 李胜杰; 于凌云; 蔡磊

    2012-01-01

    应用新一代高通量测序技术Roche 454对大口黑鲈(Micropterus salmoides)北方亚种和佛罗里达亚种进行转录组测序并建立ESTs数据库,结果得到北方亚种ESTs序列468 671条,佛罗里达亚种ESTs序列332 322条,两亚种ESTs序列的平均长度分别为306.5bp和304.4bp。将得到的高质量序列进行拼接,大口黑鲈北方亚种和佛罗里达亚种共得到contig序列总数分别为42 056条和35 743条,平均长度分别为612.6bp和588.2bp。将大口黑鲈北方亚种和佛罗里达亚种的数据库合并,通过与蛋白数据库比对后,共78 938条EST序列被注释,根据Gene Ontology(GO)信息,对序列按照分子功能、细胞组成、生物学过程进行分类。通过对大口黑鲈合并的EST库信息进行大通量SSR和SNP位点的发掘,发现了含SSRs和SNPs的序列分别为25 469和8 547条。从EST数据库中随机选取75条contigs进行北方亚种和佛罗里达亚种的序列比较,结果表明两个亚种EST序列同源性为99.2%。%In this study,Roche 454 high-throughput technology was used to conduct transcriptome sequencing and establish ESTs database of the largemouth bass northern subspecies and Florida subspecies.The results showed that a total of 468 671 and 332 322 ESTs were generated from northern subspecies and Florida subspecies.The average length was 306.5 bp and 304.4 bp,respectively.Assembly of the largemouth bass northern subspecies and Florida subspecies' high quality ESTs resulted in 42 056 and 35 743 contigs.The average length was 612.6 bp and 588.2 bp,respectively.EST database of largemouth bass northern subspecies and Florida subspecies was merged and then compared with known protein databases.78 938 EST sequences in total were annotated.By exploring the merged largemouth bass EST library,over 25 469 and 8 547 putative SSRs and SNPs were identified.Comparison of the northern subspecies and Florida subspecies sequence was made by randomly selecting 75 contigs from

  1. Anoplastie périnéale simple pour le traitement des malformations anorectales basses chez l'adulte, à propos de deux cas

    OpenAIRE

    Abdelmoughit ECHCHAOUI; Benyachou, Malika; Hafidi, Jawad; Fathi, Nahed; Mohammadine, Elhamid; ELmazouz, Samir; Gharib, Nour-eddine; Abbassi, Abdellah

    2014-01-01

    Les malformations anorectales chez l'adulte sont des anomalies congénitales rares du tube digestif qui prédominent chez le sexe féminin. Notre étude porte sur deux observations de malformation anorectale basses vues et traitées au stade adulte par les 2 équipes (plasticiens et viscéralistes) à l'Hôpital Avicenne à Rabat. Il s'agit d'un homme de 24 ans avec une dyschésie anale l'autre cas est une femme de 18 ans avec une malformation anovulvaire Les caractéristiques cliniques combinées avec le...

  2. Étude des performances du télescope gamma Fermi à basse énergie et impact sur la science

    OpenAIRE

    Desgardin, Thibaut

    2015-01-01

    Le satellite \\textit{Fermi}, lancé en juin 2008 par la NASA, observe le ciel dans le domaine des hautes énergies. Son instrument principal: le \\textit{Large Area Telescope} (LAT), récolte des données à partir de $\\sim 20$ MeV jusqu'à plus de 300 GeV. Une amélioration majeure de ses algorithmes a permis récemment la mise à disposition d'un nouveau jeu de données, Pass 8. Pass 8 jette un nouvel éclairage sur le domaine méconnu de la basse énergie (E

  3. Implementation of a non-lethal biopsy punch monitoring program for mercury in smallmouth bass, Micropterus dolomieu Lacepède, from the Eleven Point River, Missouri.

    Science.gov (United States)

    Ackerson, J R; McKee, M J; Schmitt, C J; Brumbaugh, W G

    2014-02-01

    A non-lethal biopsy method for monitoring mercury (Hg) concentrations in smallmouth bass (Micropterus dolomieu; smallmouth) from the Eleven Point River in southern Missouri USA was evaluated. A biopsy punch was used to remove a muscle tissue plug from the area immediately below the anterior dorsal fin of 31 smallmouth. An additional 35 smallmouth (controls) were held identically except that no tissue plug was removed. After sampling, all fish were held in a concrete hatchery raceway for 6 weeks. Mean survival at the end of the holding period was 97 % for both groups. Smallmouth length, weight and Fulton's condition factor at the end of the holding period were also similar between plugged and non-plugged controls, indicating that the biopsy procedure had minimal impact on growth under these conditions. Tissue plug Hg concentrations were similar to smallmouth Hg data obtained in previous years by removing the entire fillet for analysis. PMID:24196376

  4. Effet des additifs sur la structure cristalline du polyéthylène à basse densité (PEBD) irradiés par des rayonnements UV

    OpenAIRE

    Cheloufi T; Berranem M; Gasmi A; Benaissa S

    2014-01-01

    L’effet des rayons U V sur les propriétés physico-chimiques et mécaniques du polyéthylène à basse densité (PEBD) et pigmenté avec des additifs dans différentes formulations ont été étudiées. Les échantillons ont été exposés au rayonnement U V émis par une lampe à xénon d’une puissance de 6000 W à une température de 46 °C pour différents temps. Un échantillon de référence a été pris pour la comparaison. A l’aide de la spectroscopie infrarouge FTIR et l’essai de traction, on a pu suivre les cha...

  5. Cristallisation du polyéthylène basse densité : mesures rhéologiques et calorimétriques

    OpenAIRE

    Peiti, Christian; Vergnes, Bruno; Haudin, Jean-Marc

    2008-01-01

    Deux polyéthylènes basse densité, obtenus par procédé tubulaire et procédé autoclave, ont été caractérisés par chromatographie d'exclusion stérique. Leur masse molaire moyenne en masse est sensiblement identique, mais le matériau autoclave présente une queue de plus fortes masses. Ceci induit des différences notables sur les conditions de cristallisation. Les mesures de spectrométrie mécanique effectuées en fréquence et température mettent en évidence que le facteur de glissement suit une loi...

  6. Comparative pathogenicity study of ten different betanodavirus strains in experimentally infected European sea bass, Dicentrarchus labrax (L.)

    DEFF Research Database (Denmark)

    Vendramin, Niccolò; Toffan, A.; Mancin, M.;

    2014-01-01

    Viral encephalopathy and retinopathy (VER), otherwise known as viral nervous necrosis (VNN), is a severe pathological condition caused by RNA viruses belonging to the Nodaviridae family, genus Betanodavirus. The disease, described in more than 50 fish species worldwide, is considered as the most...... serious viral threat affecting marine farmed species in the Mediterranean region, thus representing one of the bottlenecks for further development of the aquaculture industry. To date, four different genotypes have been identified, namely red-spotted grouper nervous necrosis virus (RGNNV), striped jack...... trial. The investigation was conducted using the European sea bass, the first target fish species for the disease in the Mediterranean basin. Naive fish were challenged by immersion and clinical signs and mortality were recorded for 68 days; furthermore, samples collected at selected time points were...

  7. Cultivation of Microalgae Chlorella sp. and Scenedesmus sp. as a Potentional Biofuel Feedstock

    Directory of Open Access Journals (Sweden)

    Prof. dr. Violeta Makareviciene

    2011-10-01

    Full Text Available The growth of two robust algae strains Chlorella sp. and Scenedesmus sp. growing in Lithuanian lakes was investigated with the aim to obtain optimum conditions for biomass cultivation for biofuel production in the Lithuanian environment. Samples were taken from different nitrogen sources and of different concentrations, with addition of various concentrations of CO2 and in the presence of salt. The best biomass productivity was achieved using urea as a nitrogen source or modified growing medium BG11 with decreased concentration of NaNO3. The positive impact on the growth of biomass was achieved by aeration with CO2 (especially with concentration of 24%. Additional research into the removal of pollutants, such inorganic salts of nitrogen and phosphorus and organic materials from wastewater using microalgae has revealed good possibilities of using both algae strains in wastewater treatment plants. A content of oil in Chlorella sp. and Scenedesmus sp. has suggested their potential use as biodiesel feedstock.

  8. Candida flosculorum sp. nov. and Candida floris sp. nov., two yeast species associated with tropical flowers.

    Science.gov (United States)

    Rosa, Carlos A; Pagnocca, Fernando C; Lachance, Marc-André; Ruivo, Carla C C; Medeiros, Adriana O; Pimentel, Mariana R C; Fontenelle, Julio C R; Martins, Rogério P

    2007-12-01

    Two ascomycetous yeast species, Candida flosculorum sp. nov. and Candida floris sp. nov., were isolated from tropical flowers and their associated insects. C. flosculorum was isolated from flower bracts of Heliconia velloziana and Heliconia episcopalis (Heliconiaceae) collected from two Atlantic rain forest sites in Brazil. C. floris was isolated from flowers of Ipomoea sp. (Convolvulaceae) growing on the banks of the river Paraguai in the pantanal ecosystem in Brazil and from an adult of the stingless bee Trigona sp. and a flower of Merremia quinquefolia (Convolvulaceae) in Costa Rica. C. flosculorum belongs to the Metschnikowiaceae clade and C. floris belongs to the Starmerella clade. The type strain of C. flosculorum is UFMG-JL13(T) (=CBS 10566(T)=NRRL Y-48258(T)) and the type strain of C. floris is UWO(PS) 00-226.2(T) (=CBS 10593(T)=NRRL Y-48255(T)). PMID:18048759

  9. The astaxanthin dideoxyglycoside biosynthesis pathway in Sphingomonas sp. PB304

    DEFF Research Database (Denmark)

    Kim, Se Hyeuk; Kim, Jin Ho; Lee, Bun Yeol;

    2014-01-01

    A major carotenoid in Sphingomonas sp. PB304, originally isolated from a river in Daejon City, South Korea, was identified as astaxanthin dideoxyglycoside. Gene clusters encoding the astaxanthin dideoxyglycoside biosynthetic enzymes were identified by screening Sphingomonas sp. PB304 fosmid...... of astaxanthin dideoxyglycoside in Sphingomonas sp. PB304, a putative corresponding gene in the carotenoid biosynthetic gene cluster, and high amino acid sequence homology to the existing glycosyltransferases. Therefore, we propose that astaxanthin dideoxyglycoside can be synthesized in Sphingomonas sp. PB304...

  10. Effects of fish oil replacement by vegetable oil blend on digestive enzymes and tissue histomorphology of European sea bass (Dicentrarchus labrax) juveniles.

    Science.gov (United States)

    Castro, Carolina; Couto, Ana; Pérez-Jiménez, Amalia; Serra, Cláudia R; Díaz-Rosales, Patricia; Fernandes, Rui; Corraze, Geneviève; Panserat, Stéphane; Oliva-Teles, Aires

    2016-02-01

    The impact of replacing circa 70% fish oil (FO) by a vegetable oil (VO) blend (rapeseed, linseed, palm oils; 20:50:30) in diets for European sea bass juveniles (IBW 96 ± 0.8 g) was evaluated in terms of activities of digestive enzymes (amylase, lipase, alkaline phosphatase, trypsin and total alkaline proteases) in the anterior (AI) and posterior (PI) intestine and tissue morphology (pyloric caeca-PC, AI, PI, distal intestine-DI and liver). For that purpose, fish were fed the experimental diets for 36 days and then liver and intestine were sampled at 2, 6 and 24 h after the last meal. Alkaline protease characterization was also done in AI and PI at 6 h post-feeding. Dietary VO promoted higher alkaline phosphatase activity at 2 h post-feeding in the AI and at all sampling points in the PI. Total alkaline protease activity was higher at 6 h post-feeding in the PI of fish fed the FO diet. Identical number of bands was observed in zymograms of alkaline proteases of fish fed both diets. No alterations in the histomorphology of PC, AI, PI or DI were noticed in fish fed the VO diets, while in the liver a tendency towards increased hepatocyte vacuolization due to lipid accumulation was observed. Overall, and with the exception of a higher intestine alkaline phosphatase activity, 70% FO replacement by a VO blend in diets for European sea bass resulted in no distinctive alterations on the postprandial pattern of digestive enzyme activities and intestine histomorphology. PMID:26364216

  11. Genomic characterization of the European sea bass Dicentrarchus labrax reveals the presence of a novel uncoupling protein (UCP gene family member in the teleost fish lineage

    Directory of Open Access Journals (Sweden)

    Tine Mbaye

    2012-05-01

    Full Text Available Abstract Background Uncoupling proteins (UCP are evolutionary conserved mitochondrial carriers that control energy metabolism and therefore play important roles in several physiological processes such as thermogenesis, regulation of reactive oxygen species (ROS, growth control, lipid metabolism and regulation of insulin secretion. Despite their importance in various physiological processes, their molecular function remains controversial. The evolution and phylogenetic distribution may assist to identify their general biological function and structure-function relationships. The exact number of uncoupling protein genes in the fish genome and their evolution is unresolved. Results Here we report the first characterisation of UCP gene family members in sea bass, Dicentrarchus labrax, and then retrace the evolution of the protein family in vertebrates. Four UCP genes that are shared by five other fish species were identified in sea bass genome. Phylogenetic reconstitution among vertebrate species and synteny analysis revealed that UCP1, UCP2 and UCP3 evolved from duplication events that occurred in the common ancestor of vertebrates, whereas the novel fourth UCP originated specifically in the teleost lineage. Functional divergence analysis among teleost species revealed specific amino acid positions that have been subjected to altered functional constraints after duplications. Conclusions This work provides the first unambiguous evidence for the presence of a fourth UCP gene in teleost fish genome and brings new insights into the evolutionary history of the gene family. Our results suggest functional divergence among paralogues which might result from long-term and differential selective pressures, and therefore, provide the indication that UCP genes may have diverse physiological functions in teleost fishes. Further experimental analysis of the critical amino acids identified here may provide valuable information on the physiological functions of

  12. Isolation and characterization of Ff1 and Gsdf family genes in European sea bass and identification of early gonadal markers of precocious puberty in males.

    Science.gov (United States)

    Crespo, Berta; Gómez, Ana; Mazón, María José; Carrillo, Manuel; Zanuy, Silvia

    2013-09-15

    Puberty represents the transition from an immature to a mature reproductive stage. The mechanisms underlying the onset of normal or precocious puberty have not yet been elucidated. With the goal of gaining an understanding of early events that occur in the testes of precocious animals during this process, a hemigonadectomy was performed on male juvenile sea bass and expression levels of candidate mRNAs were determined through quantitative real-time RT-PCR. For this purpose, the gonadal soma-derived factors gsdf1 and gsdf2, the nuclear receptor 5 subfamily members nr5a1a (ff1b), nr5a1b (ff1d), nr5a2 (ff1a) and nr5a5 (ff1c) and the proliferating cell nuclear antigen or pcna, genes with a putative role in the beginning of spermatogenesis, were isolated and cloned. Hemigonadectomy proved to be a suitable strategy for the study of gonadal stages prior to the appearance of histological differences between precocious and non-precocious fish, as it allowed the subsequent classification of these gonads. The upregulation of the gene encoding the steroidogenic acute regulatory protein (Star) in precocious testes indicates that sex steroids could play a role in the onset of spermatogenesis in sea bass. In contrast, the downregulation observed in ff1b expression indicates that this initial surge in star expression is not the result of Ff1b transactivation, suggesting an alternative pathway for this transcriptional activation. Finally, a decrease in gsdf1 expression in precocious animals suggests that this gene may play a role in the onset of puberty, while its correlation with ff1b expression points to gsdf1 as a putative target for Ff1b-mediated transactivation. PMID:23791759

  13. Biodegradation of hexavalent chromium (Cr+6) in wastewater using Pseudomonas sp. and Bacillus sp. bacterial strains

    OpenAIRE

    Muhammad Qasim

    2013-01-01

    The recovery of toxic metal compounds is a deep concern in all industries. Hexavalent chromium is particularly worrying because of its toxic influence on human health. In this paper, biodegradation of hexavalent chromium (Cr+6) present in wastewater has been studied using two different bacterial strains; Pseudomonas sp. and Bacillus sp. A chemostat (with and without recycle of cells) with 10 L liquid culture volume was used to study the substrate and the biomass cell concentrations with time....

  14. Coronamycins, peptide antibiotics produced by a verticillate Streptomyces sp. (MSU-2110) endophytic on Monstera sp.

    OpenAIRE

    Ezra, D; Castillo, UF; Strobel, GA; Hess, WM; Porter, H; Jensen, JB; Condron, MAM; Teplow, DB; J. Sears; Maranta, M; Hunter, M; Weber, B.; Yaver, D

    2004-01-01

    Coronamycin is a complex of novel peptide antibiotics with activity against pythiaceous fungi and the human fungal pathogen Cryptococcus neoformans. It is also active against the malarial parasite, Plasmodium falciparum, with an IC50 of 9.0 ng ml-1. Coronamycin is produced by a verticillate Streptomyces sp. isolated as an endophyte from an epiphytic vine, Monstera sp., found in the Manu region of the upper Amazon of Peru. Bioassay-guided fractionation of the fermentation broths of this endoph...

  15. The technique of autoradiography at very low temperature; Technique d'autoradiographie a tres basse temperature

    Energy Technology Data Exchange (ETDEWEB)

    Pellerin, P. [Commissariat a l' Energie Atomique, Saclay (France).Centre d' Etudes Nucleaires

    1959-07-01

    ferme. Le developpement a lieu a temperature ordinaire et la sensibilite de l'emulsion n'est pas appreciabdement modifiee. Cette technique permet de maintenir le bloc tres au-dessous de la temperature de congelation, des la mort de l'animal jusqu'au developpement; toute diffusion chimique est supprimee et la repartition de la substance radioactive saisie sur l'autoradiographie a l'instant exact de la mort par congelation. Des fraisages successifs permettent d'etudier la repartition de la substance radioactive, a toutes les profondeurs. L'absence d'effets pseudo-radiographiques est totale. Enfin, le renforcement des couleurs naturelles des elements anatomiques par cette tres basse temperature est considerable, ce qui permet de realigner l'autoradiographie en couleurs de le coupe processus de coloration. (auteur)

  16. Microbiologically influenced corrosion of galvanized steel by Desulfovibrio sp. and Desulfosporosinus sp. in the presence of Ag–Cu ions

    Energy Technology Data Exchange (ETDEWEB)

    Ilhan-Sungur, Esra, E-mail: esungur@istanbul.edu.tr [Istanbul University, Faculty of Science, Department of Biology, 34134 Vezneciler, Istanbul (Turkey); Unsal-Istek, Tuba [Istanbul University, Faculty of Science, Department of Biology, 34134 Vezneciler, Istanbul (Turkey); Cansever, Nurhan [Yıldız Technical University, Faculty of Chemistry-Metallurgy, Metallurgical and Materials Engineering Department, 34210 Esenler, Istanbul (Turkey)

    2015-07-15

    The effects of Ag–Cu ions on the microbiologically induced corrosion of galvanized steel in the presence of Desulfovibrio sp. and Desulfosporosinus sp. were investigated. The corrosion behavior of galvanized steel was analyzed by potentiodynamic polarization and electrochemical impedance spectroscopy. The biofilm, corrosion products and Ag–Cu ions on the surfaces were investigated by using scanning electron microscopy, energy dispersive X-ray spectrometry and elemental mapping. The biofilm layer formed by the Desulfovibrio sp. was stable covering the all surface of galvanized steel coupons, while that by Desulfosporosinus sp. was intermittent, highly porous and heterogeneous. It was found that both of the sulfate reducing bacteria species accelerated corrosion of the galvanized steel. However, it was detected that Desulfosporosinus sp. was more corrosive for galvanized steel than Desulfovibrio sp. Scanning electron microscopy images showed that Desulfovibrio sp. and Desulfosporosinus sp. in biofilm clustered into patches on the galvanized steel surface when the culture contained toxic Ag–Cu ions. The ions affected the growth of the sulfate reducing bacteria strains in different ways and hence the corrosion behaviors. It was observed that the Ag–Cu ions affected negatively growth of Desulfosporosinus sp. especially after 24 h of exposure leading to a decrease in the corrosion rate of galvanized steel. However, Desulfovibrio sp. showed more corrosive effect in the presence of the ions according to the ions-free culture. Energy dispersive X-ray spectrometry analysis showed that corrosion products on the surfaces were mainly composed of Zn, S, Na, O and P. - Highlights: • Galvanized steel was corroded by Desulfosporosinus sp. and Desulfovibrio sp. • Desulfosporosinus sp. is more corrosive than Desulfovibrio sp. • The Ag–Cu ions affected corrosion behavior of Desulfosporosinus sp. and Desulfovibrio sp. on galvanized steel.

  17. Microbiologically influenced corrosion of galvanized steel by Desulfovibrio sp. and Desulfosporosinus sp. in the presence of Ag–Cu ions

    International Nuclear Information System (INIS)

    The effects of Ag–Cu ions on the microbiologically induced corrosion of galvanized steel in the presence of Desulfovibrio sp. and Desulfosporosinus sp. were investigated. The corrosion behavior of galvanized steel was analyzed by potentiodynamic polarization and electrochemical impedance spectroscopy. The biofilm, corrosion products and Ag–Cu ions on the surfaces were investigated by using scanning electron microscopy, energy dispersive X-ray spectrometry and elemental mapping. The biofilm layer formed by the Desulfovibrio sp. was stable covering the all surface of galvanized steel coupons, while that by Desulfosporosinus sp. was intermittent, highly porous and heterogeneous. It was found that both of the sulfate reducing bacteria species accelerated corrosion of the galvanized steel. However, it was detected that Desulfosporosinus sp. was more corrosive for galvanized steel than Desulfovibrio sp. Scanning electron microscopy images showed that Desulfovibrio sp. and Desulfosporosinus sp. in biofilm clustered into patches on the galvanized steel surface when the culture contained toxic Ag–Cu ions. The ions affected the growth of the sulfate reducing bacteria strains in different ways and hence the corrosion behaviors. It was observed that the Ag–Cu ions affected negatively growth of Desulfosporosinus sp. especially after 24 h of exposure leading to a decrease in the corrosion rate of galvanized steel. However, Desulfovibrio sp. showed more corrosive effect in the presence of the ions according to the ions-free culture. Energy dispersive X-ray spectrometry analysis showed that corrosion products on the surfaces were mainly composed of Zn, S, Na, O and P. - Highlights: • Galvanized steel was corroded by Desulfosporosinus sp. and Desulfovibrio sp. • Desulfosporosinus sp. is more corrosive than Desulfovibrio sp. • The Ag–Cu ions affected corrosion behavior of Desulfosporosinus sp. and Desulfovibrio sp. on galvanized steel

  18. Effects of lung surfactant proteins, SP-B and SP-C, and palmitic acid on monolayer stability.

    OpenAIRE

    Ding, J; Takamoto, D Y; von Nahmen, A; Lipp, M M; Lee, K Y; Waring, A J; Zasadzinski, J A

    2001-01-01

    Langmuir isotherms and fluorescence and atomic force microscopy images of synthetic model lung surfactants were used to determine the influence of palmitic acid and synthetic peptides based on the surfactant-specific proteins SP-B and SP-C on the morphology and function of surfactant monolayers. Lung surfactant-specific protein SP-C and peptides based on SP-C eliminate the loss to the subphase of unsaturated lipids necessary for good adsorption and respreading by inducing a transition between...

  19. Pulmonary surfactant protein A (SP-A) specifically binds dipalmitoylphosphatidylcholine

    International Nuclear Information System (INIS)

    Phospholipids are the major components of pulmonary surfactant. Dipalmitoylphosphatidylcholine is believed to be especially essential for the surfactant function of reducing the surface tension at the air-liquid interface. Surfactant protein A (SP-A) with a reduced denatured molecular mass of 26-38 kDa, characterized by a collagen-like structure and N-linked glycosylation, interacts strongly with a mixture of surfactant-like phospholipids. In the present study the direct binding of SP-A to phospholipids on a thin layer chromatogram was visualized using 125I-SP-A as a probe, so that the phospholipid specificities of SP-A binding and the structural requirements of SP-A and phospholipids for the binding could be examined. Although 125I-SP-A bound phosphatidylcholine and sphingomyeline, it was especially strong in binding dipalmitoylphosphatidylcholine, but failed to bind phosphatidylglycerol, phosphatidylinositol, phosphatidylethanolamine, and phosphatidylserine. Labeled SP-A also exhibited strong binding to distearoylphosphatidylcholine, but weak binding to dimyristoyl-, 1-palmitoyl-2-linoleoyl-, and dilinoleoylphosphatidylcholine. Unlabeled SP-A readily competed with labeled SP-A for phospholipid binding. SP-A strongly bound dipalmitoylglycerol produced by phospholipase C treatment of dipalmitoylphosphatidylcholine, but not palmitic acid. This protein also failed to bind lysophosphatidylcholine produced by phospholipase A2 treatment of dipalmitoylphosphatidylcholine. 125I-SP-A shows almost no binding to dipalmitoylphosphatidylglycerol and dipalmitoylphosphatidylethanolamine. The addition of 10 mM EGTA into the binding buffer reduced much of the 125I-SP-A binding to phospholipids. Excess deglycosylated SP-A competed with labeled SP-A for binding to dipalmitoylphosphatidylcholine, but the excess collagenase-resistant fragment of SP-A failed

  20. Fitoremediasi limbah budidaya sidat menggunakan filamentous algae (Spirogyra sp.

    Directory of Open Access Journals (Sweden)

    Tri Apriadi

    2014-09-01

    Full Text Available Penelitian ini bertujuan untuk mengetahui potensi dari filamentous algae (Spirogyra sp. sebagai agen bioremediasi dalam mereduksi kandungan bahan organik limbah budidaya sidat. Penelitian menggunakan rancangan acak lengkap dengan perlakuan perbedaan dosis limbah (25 %, 50 %, 75 %, 100%. Wadah penelitian berupa akuarium resirkulasi menggunakan sistem carrousel. Dilakukan pengukuran secara rutin terhadap beberapa parameter kualitas air serta perubahan bobot Spirogyra sp. selama dua minggu retensi. Diperoleh hasil bahwa penurunan konsentrasi bahan organik menggunakan Spirogyra sp. berlangsung efektif hingga hari keenam. Spirogyra sp. mampu mentolelir limbah budidaya sidat pada dosis limbah 25% dan 50%. Spirogyra sp. pada perlakuan dosis limbah 50% memiliki kemampuan yang lebih baik dalam menurunkan bahan organik limbah budidaya sidat.

  1. Substitusi Dedak Padi Dengan Pod Kakao(Theobroma cacao L) Dipermentasi Dengan Rhizopus SP, Saccharomyces SP, Lactobacilus SP Terhadap Performans Ternak Babi Perternakan Larance Jantan

    OpenAIRE

    Bornok Venantius P.

    2013-01-01

    BORNOK VENANTIUS P: Substitution of rice bran with cacao pods fermented by Rhizopus sp, Saccharomyces sp and Lactobacillus sp to performance of male croosbred Landrace swine. Under the supervision by NURZAINNAH GINTING and ISKANDAR SEMBIRING. Cacao pods fermented can be used as an alternative feed a substitution of rice bran for its capacity to improve performance during growth. The objective of this research was to prove potention of cacao pods fermented, which can be seen from consumptio...

  2. Shelf-life extension of refrigerated sea bass slices wrapped with fish protein isolate/fish skin gelatin-ZnO nanocomposite film incorporated with basil leaf essential oil.

    Science.gov (United States)

    Arfat, Yasir Ali; Benjakul, Soottawat; Vongkamjan, Kitiya; Sumpavapol, Punnanee; Yarnpakdee, Suthasinee

    2015-10-01

    Microbiological, chemical and sensory changes of sea bass slices wrapped with fish protein isolate (FPI)/fish skin gelatin (FSG) films incorporated with 3 % ZnO nanoparticles (ZnONP) (w/w, based on protein content) and 100 % basil leaf essential oil (BEO) (w/w, based on protein content) during storage of 12 days at 4 °C were investigated. Sea bass slices wrapped with FPI/FSG-ZnONP-BEO film had the lowest growth of psychrophilic bacteria, lactic acid bacteria and spoilage microorganisms including Pseudomonas , H2S-producing bacteria and Enterobacteriaceae throughout storage of 12 days in comparison with those wrapped with FPI/FSG-BEO, FPI/FSG-ZnONP, FPI/FSG film, polypropylene film (PP film) and the control (without wrapping), respectively (P control (6 days) (P < 0.05). PMID:26396365

  3. A pumilio homolog in Polycelis sp.

    Science.gov (United States)

    Yuwen, Yanqing; Dong, Zimei; Si, Xiaohui; Chen, Guangwen

    2014-02-01

    Pumilio proteins (PUMs), members of the pumilio/fem-3 mRNA-binding factor (PUF) family, are eukaryote-specific RNA-binding proteins. We isolated a 2,048-basepair cDNA fragment of a pumilio homolog from the planarian flatworm Polycelis sp. This pumilio protein (PyPUM) contains a conserved pumilio homology domain (PUM-HD) consisting of eight repeats and two flanking half repeats. PyPUM shows high similarity to Dugesia japonica pumilio (DjPUM) from another planarian D. japonica, and their PUM-HD also shows high similarity to each other. Furthermore, our data showed that there is a flatworm-specific spacer between repeats 7 and 8. Phylogenetic analysis showed that PyPUM has a closer relationship to other PUM homologs from flatworms. These results provide a foundation for future functional studies of pumilio gene in Polycelis sp. PMID:24292205

  4. Sp6 and Sp8 transcription factors control AER formation and dorsal-ventral patterning in limb development.

    Directory of Open Access Journals (Sweden)

    Endika Haro

    2014-08-01

    Full Text Available The formation and maintenance of the apical ectodermal ridge (AER is critical for the outgrowth and patterning of the vertebrate limb. The induction of the AER is a complex process that relies on integrated interactions among the Fgf, Wnt, and Bmp signaling pathways that operate within the ectoderm and between the ectoderm and the mesoderm of the early limb bud. The transcription factors Sp6 and Sp8 are expressed in the limb ectoderm and AER during limb development. Sp6 mutant mice display a mild syndactyly phenotype while Sp8 mutants exhibit severe limb truncations. Both mutants show defects in AER maturation and in dorsal-ventral patterning. To gain further insights into the role Sp6 and Sp8 play in limb development, we have produced mice lacking both Sp6 and Sp8 activity in the limb ectoderm. Remarkably, the elimination or significant reduction in Sp6;Sp8 gene dosage leads to tetra-amelia; initial budding occurs, but neither Fgf8 nor En1 are activated. Mutants bearing a single functional allele of Sp8 (Sp6-/-;Sp8+/- exhibit a split-hand/foot malformation phenotype with double dorsal digit tips probably due to an irregular and immature AER that is not maintained in the center of the bud and on the abnormal expansion of Wnt7a expression to the ventral ectoderm. Our data are compatible with Sp6 and Sp8 working together and in a dose-dependent manner as indispensable mediators of Wnt/βcatenin and Bmp signaling in the limb ectoderm. We suggest that the function of these factors links proximal-distal and dorsal-ventral patterning.

  5. Taxonomy Icon Data: Synechocystis sp.PCC 6803 [Taxonomy Icon

    Lifescience Database Archive (English)

    Full Text Available Synechocystis_sp_PCC_6803_NL.png Synechocystis_sp_PCC_6803_S.png Synechocystis_sp_PCC_6803_NS.png http://biosciencedbc.jp/taxonomy..._icon/icon.cgi?i=Synechocystis+sp%2ePCC+6803&t=L http://biosciencedbc.jp/taxonomy_ico...n/icon.cgi?i=Synechocystis+sp%2ePCC+6803&t=NL http://biosciencedbc.jp/taxonomy_ic...on/icon.cgi?i=Synechocystis+sp%2ePCC+6803&t=S http://biosciencedbc.jp/taxonomy_icon/icon.cgi?i=Synechocystis...+sp%2ePCC+6803&t=NS http://togodb.biosciencedbc.jp/togodb/view/taxonomy_icon_comment_en?species_id=69 ...

  6. Analysis list: SP2 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available SP2 Blood,Liver,Pluripotent stem cell + hg19 http://dbarchive.biosciencedbc.jp/kyus...hu-u/hg19/target/SP2.1.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/target/SP2.5.tsv http://dbarchive.bioscience...dbc.jp/kyushu-u/hg19/target/SP2.10.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/colo/SP2.B...lood.tsv,http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/colo/SP2.Liver.tsv,http://dbarchive.bioscience...dbc.jp/kyushu-u/hg19/colo/SP2.Pluripotent_stem_cell.tsv http://dbarchive.biosciencedbc

  7. Commercial technologies from the SP-100 program

    International Nuclear Information System (INIS)

    For more than a decade, Jet Propulsion Labortory and Los Alamos have managed a multi-agency funded effort to develop a space reactor power system. This SP-100 Program has developed technologies required for space power systems that can be implemented in the industrial and commercial sectors to improve competitiveness in the global economy. Initial steps taken to transfer this technology from the laboratories to industrial and commercial entities within United States include: (1) identifying specific technologies having commercial potential; (2) distributing information describing the identified technologies and interacting with interested commercial and industrial entities to develop application-specific details and requirements; and (3) providing a technological data base that leads to transfer of technology or the forming of teaming arrangements to accomplish the transfer by tailoring the technology to meet application-specific requirements. SP-100 technologies having commercial potential encompass fabrication processes, devices, and components. Examples: a process for bonding refractory metals to graphite, a device to sense the position of an actuator and a component to enable rotating machines to operate without supplying lubrication (self-lubricating ball bearing). Shortly after the NASA Regional Technology Transfer Centers widely disseminated information covering SP-100 technologies, over one hundred expressions of interest were received, which indicate that there is a large potential benefit in transferring SP-100 technology. Interactions with industrial and commercial entities have identified a substantial need for creating teaming arrangements involving the interested entity and personnel from laboratories and their contractors, who have the knowledge and ability to tailor the technology to meet application-specific requirements. copyright 1995 American Institute of Physics

  8. Carbon sp chains in graphene nanoholes

    Science.gov (United States)

    Castelli, Ivano E.; Ferri, Nicola; Onida, Giovanni; Manini, Nicola

    2012-03-01

    Nowadays sp carbon chains terminated by graphene or graphitic-like carbon are synthesized routinely in several nanotech labs. We propose an ab initio study of such carbon-only materials, by computing their structure and stability, as well as their electronic, vibrational and magnetic properties. We adopt a fair compromise of microscopic realism with a certain level of idealization in the model configurations, and predict a number of properties susceptible to comparison with experiment.

  9. Uptake of Glyphosate by an Arthrobacter sp

    OpenAIRE

    Pipke, Rüdiger; Schulz, Arno; Amrhein, Nikolaus

    1987-01-01

    The uptake of glyphosate (N-[phosphonomethyl]glycine) by an Arthrobacter sp. which can utilize this herbicide as its sole source of phosphorus was investigated. Orthophosphate suppressed the expression of the uptake system for glyphosate and also competed with glyphosate for uptake. The Km for glyphosate uptake was 125 μM, and the Ki for orthophosphate was 24 μM. Organophosphonates as well as organophosphates inhibited glyphosate uptake, but only organophosphates and orthophosphate suppressed...

  10. Spøgelsets egne ord

    DEFF Research Database (Denmark)

    Gemzøe, Anker

    2012-01-01

    refleksioner over den gengangerscene, der åbner Shakespeares Hamlet. Ifølge Derridas ’hantologie’ opererer spøgelser i en eksistentiel tidslighed, der har at gøre med sorg, sprog og forvandling. Genremæssigt, tilføjer Gemzøe, har gengangeren det med at husere i tragedien. Med Derridas ’hantologie’ som en af de...

  11. Blastocystis sp. from food animals in India

    OpenAIRE

    Sreekumar, C.; Selvaraj, J.; Gomathinayagam, S.; Thangapandiyan, M.; Ravikumar, G.; Roy, Parimal; C Balachandran

    2013-01-01

    Blastocystis, a zoonotic protozoan found in the intestinal tracts of a wide range of animals, has not been reported from non-human hosts from India so far. Organisms indistinguishable from Blastocystis sp. were identified in the Giemsa stained intestinal scrapings collected from carcasses of piglet and poultry that were brought for necropsy to the Central University Laboratory, Chennai. The ‘central vacuole forms’ of the parasite, with number of nuclei ranging from 1 to 12 were identified. Th...

  12. Chlamydomonas sajao nov. sp. (Chlorophyta, Volvocales)

    Science.gov (United States)

    Lewin, Ralph A.

    1984-06-01

    A new species of Chlamydomonas, namely, C. sajao nov. sp. of the Volvocales, Chlorophyta was isolated from a duckweed growing near a ricefield in the vicinity of Guangzhou, China. This interesting unicellular green alga, similar to C. mexicana from Mexico, secretes quantities of extracellular mucilaginous polysaccharides, and may be employed in improving soil quality. The new species resembles C. waldenburgensis Moewus in most characteristics but differs in three important features.

  13. Celtis sp MICROPROPAGATION: CONTAMINATION AND OXIDATION CONTROL

    OpenAIRE

    Aurora Yoshiko Sato; Herly Carlos Teixeira Dias; Leonaldo Alves de Andrade; Vênia Camello de Souza

    2001-01-01

    Tissue culture presents great potential for the establishment of native species in vitro, which have economic-ecological importance. The Celtis sp or "juazeiro-de-bode" is a semi-arid plant from Northeastern Brazil used as forage during the long dry season. Not much is known about its reproduction, and the micropropagation may represent one alternative for its propagation. The oxidation and contamination are the main problems in establishment, in vitro, of any part of plants. For studying the...

  14. Oxidation of acyclic terpenoids by Corynebacterium sp.

    OpenAIRE

    Yamada, Y; Seo, C W; Okada, H.

    1985-01-01

    Squalene analogs such as lycopersene, geranylfarnesyl, digeranyl, and 2-hydroxy-2,3-dihydrosqualene and terpene alcohol derivatives such as farnesyl benzyl ether, farnesyl pivalate, geranylgeranyl pivalate, geranyl pivalate, and geranyl benzyl ether were oxidized by Corynebacterium sp. strain SY-79, which was isolated from soil by using squalene as a carbon source. Lycopersene and geranylfarnesyl gave no major product. Digeranyl, geranyl benzyl ether, and geranyl pivalate gave terminal oxidat...

  15. Biosorption of 241Am by Candida sp

    International Nuclear Information System (INIS)

    The biosorption of radionuclide 241Am from solutions by Candida sp., and the influences of experimental conditions on the adsorption were studied. The results showed that the adsorption equilibrium was achieved within 4h and the optimum pH=2. No significant differences on 241Am biosorption were observed at 10-45 degree C, or challenged with Au3+ or Ag+, even 1500 times or 4500 times over 241Am, respectively. The adsorption rate could reach 97.8% by dry Candida sp. of 0.82 g/L in 241Am solutions (pH=2) of 5.6-111 MBq/L (44.04-873.0 μg/L) (C0), with maximum adsorption capacity (W) of 63.5 MBq/g (501.8 μg/g), implying that the removal of 241Am by Candida sp. from solutions was feasible. The relationship between activities (C0) and adsorption capacities (W) of 241Am indicated that the biosorption process could be described by Langmuir adsorption isotherm

  16. Sterols from the Madagascar Sponge Fascaplysinopsis sp.

    Directory of Open Access Journals (Sweden)

    Yoel Kashman

    2010-12-01

    Full Text Available The sponge Fascaplysinopsis sp. (order Dictyoceratida, Family Thorectidae from the west coast of Madagascar (Indian Ocean is a particularly rich source of bioactive nitrogenous macrolides. The previous studies on this organism led to the suggestion that the latter should originate from associated microsymbionts. In order to evaluate the influence of microsymbionts on lipid content, 10 samples of Fascaplysinopsis sp. were investigated for their sterol composition. Contrary to the secondary metabolites, the sterol patterns established were qualitatively and quantitatively stable: 14 sterols with different unsaturated nuclei, D5, D7 and D5,7, were identified; the last ones being the main sterols of the investigated sponges. The chemotaxonomic significance of these results for the order Dictyoceratida is also discussed in the context of the literature. The conjugated diene system in D5,7 sterols is known to be unstable and easily photo-oxidized during storage and/or experiments to produce 5a,8a-epidioxy sterols. However, in this study, no 5a,8a-epidioxysterols (or only trace amounts were observed. Thus, it was supposed that photo-oxidation was avoided thanks to the natural antioxidants detected in Fascaplysinopsis sp. by both the DPPH and b-caroten bleaching assays.

  17. Sarcocystis sp. encephalomyelitis in a cat.

    Science.gov (United States)

    Bisby, Tricia M; Holman, Patricia J; Pitoc, George A; Packer, Rebecca A; Thompson, Craig A; Raskin, Rose E

    2010-03-01

    A 5-month-old male neutered domestic shorthair cat was evaluated for spinal pain, ataxia, and anisocoria. Neuroanatomic localization indicated diffuse or multifocal central nervous system disease. On cerebrospinal fluid analysis, neutrophilic pleocytosis and intracellular protozoal merozoites were observed. The merozoites were oval, 2-4 microm in width and 4-6 microm in length, and had linear arrays of nuclear material concentrated at one pole. Serum was positive for Sarcocystis sp. antibodies and negative for Toxoplasma gondii antibodies. The organism was determined to be either Sarcocystis neurona or Sarcocystis dasypi based on sequence analysis of the internal transcribed spacer 1 ribosomal RNA genomic region. Clinical disease resolved following treatment with 3 different protocols for protozoal infection. This case is the first to demonstrate the antemortem diagnosis and survival of a domestic cat with Sarcocystis sp.-associated encephalomyelitis. Clinicians and cytopathologists should include Sarcocystis sp. as a differential for feline inflammatory central nervous system disease characterized by neutrophilic pleocytosis. PMID:19548967

  18. Record Of Both Tectonic Related Vertical Motions and Global Sea Level Rise by Marine Terraces along an Active Arc Volcano. Example of Basse-Terre, Lesser Antilles (French West-Indies).

    Science.gov (United States)

    Fabre, M.; Moysan, M.; Graindorge, D.; Jean-Frederic, L.; Philippon, M. M.; Marcaillou, B.; Léticée, J. L.

    2015-12-01

    Volcano-tectonic history of the Caribbean plate provides direct insight onto the dynamic of the North American Plate westward subduction. Basse-Terre Island is a volcanic chain that belongs to the Lesser Antilles active volcanic arc with a southward decreasing age of volcanism from 3 Ma to present day.We investigate records of vertical motion along Basse-Terre through a morphostructural analysis of the Pleistocene-Holocene shallow-water carbonate platforms and associated terraces that surround Basse-Terre Island. This study is based on new high-resolution bathymetric and dense seismic data acquired during the GEOTREF oceanographic survey (2015, February). Our bathymetric and topographic Digital Terrain Model together with the "Litto3D" Lidar data (IGN/SHOM) images the island topography and the platform bathymetry to a depth of 200m with horizontal and vertical resolutions of 5m and ~cm respectively. This detailed study highlights the morphostructure of terraces built during the last transgression in order to identify and quantify their vertical motions. We analyze inherited morphology and structures of the forearc that affect the platform to discuss effects of the regional tectonics context. A particular emphasis is put on the influence of the NW-SE arc parallel transtensive Montserrat-Bouillante fault system onto the platform geometry. At last, the distribution of Basse-Terre terraces is compared with terraces distribution around other Lesser Antilles island and the Bahamas stable margin platform. We aim at discriminating the influence of the Pleistocene global sea-level rise from the one of tectonic vertical deformations.

  19. Comunicato stampa della IARC N°238: "L’ ESPOSIZIONE A BASSE DOSI DI RADIAZIONI IONIZZANTI AUMENTA IL RISCHIO DI MORTE A CAUSA DI TUMORI SOLIDI". Pubblicato il 21 ottobre 2015

    OpenAIRE

    Fellone, Lucio

    2016-01-01

    Versione autorizzata in italiano del Comunicato Stampa della IARC N°238 pubblicato il 21 ottobre 2015: "L’ ESPOSIZIONE A BASSE DOSI DI RADIAZIONI IONIZZANTI AUMENTA IL RISCHIO DI MORTE A CAUSA DI TUMORI SOLIDI". Documento originale all'indirizzo: http://www.iarc.fr/en/media-centre/pr/2015/pdfs/pr238_E.pdf Authorized Italian version of the IARC Press Release N°238 published on 21 October 2015: Low doses of ionizing radiation increase risk of death from solid cancers...

  20. Effects of persistent organic pollutants on the thyroid function of the European sea bass (Dicentrarchus labrax) from the Aegean sea, is it an endocrine disruption?

    OpenAIRE

    Schnitzler, J.G; Koutrakis, E.T.; Siebert, U; Thomé, J.-P.; Das, K.

    2008-01-01

    We evaluated the alterations of organochlorinated compounds such as polychlorobiphenyls (PCB), dichloro-diphenyl-dichloroethylene (DDE) and dichloro-diphenyl-trichloroethane (DDT) on the thyroid in wild and cultured sea bass (Dicentrarchus labrax) at environmental concentrations. These compounds influence the endocrine system of many fish species and are qualified as endocrine disruptors. The thyroid seems to be a target organ. Two alteration endpoints: the thyroid histology and the muscular ...