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Sample records for basin monkeypox viruses

  1. Comparison of West African and Congo Basin monkeypox viruses in BALB/c and C57BL/6 mice.

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    Christina L Hutson

    Full Text Available Although monkeypox virus (MPXV studies in wild rodents and non-human primates have generated important knowledge regarding MPXV pathogenesis and inferences about disease transmission, it might be easier to dissect the importance of virulence factors and correlates of protection to MPXV in an inbred mouse model. Herein, we compared the two clades of MPXV via two routes of infection in the BALB/c and C57BL/6 inbred mice strains. Our studies show that similar to previous animal studies, the Congo Basin strain of MPXV was more virulent than West African MPXV in both mouse strains as evidenced by clinical signs. Although animals did not develop lesions as seen in human MPX infections, localized signs were apparent with the foot pad route of inoculation, primarily in the form of edema at the site of inoculation; while the Congo Basin intranasal route of infection led to generalized symptoms, primarily weight loss. We have determined that future studies with MPXV and laboratory mice would be very beneficial in understanding the pathogenesis of MPXV, in particular if used in in vivo imaging studies. Although this mouse model may not suffice as a model of human MPX disease, with an appropriate inbred mouse model, we can unravel many unknown aspects of MPX pathogenesis, including virulence factors, disease progression in rodent hosts, and viral shedding from infected animals. In addition, such a model can be utilized to test antivirals and the next generation of orthopoxvirus vaccines for their ability to alter the course of disease.

  2. Susceptibility of Marmosets (Callithrix jacchus to Monkeypox Virus: A Low Dose Prospective Model for Monkeypox and Smallpox Disease.

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    Eric M Mucker

    Full Text Available Although current nonhuman primate models of monkeypox and smallpox diseases provide some insight into disease pathogenesis, they require a high titer inoculum, use an unnatural route of infection, and/or do not accurately represent the entire disease course. This is a concern when developing smallpox and/or monkeypox countermeasures or trying to understand host pathogen relationships. In our studies, we altered half of the test system by using a New World nonhuman primate host, the common marmoset. Based on dose finding studies, we found that marmosets are susceptible to monkeypox virus infection, produce a high viremia, and have pathological features consistent with smallpox and monkeypox in humans. The low dose (48 plaque forming units required to elicit a uniformly lethal disease and the extended incubation (preclinical signs are unique features among nonhuman primate models utilizing monkeypox virus. The uniform lethality, hemorrhagic rash, high viremia, decrease in platelets, pathology, and abbreviated acute phase are reflective of early-type hemorrhagic smallpox.

  3. Laboratory Investigations of African Pouched Rats (Cricetomys gambianus as a Potential Reservoir Host Species for Monkeypox Virus.

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    Christina L Hutson

    Full Text Available Monkeypox is a zoonotic disease endemic to central and western Africa, where it is a major public health concern. Although Monkeypox virus (MPXV and monkeypox disease in humans have been well characterized, little is known about its natural history, or its maintenance in animal populations of sylvatic reservoir(s. In 2003, several species of rodents imported from Ghana were involved in a monkeypox outbreak in the United States with individuals of three African rodent genera (Cricetomys, Graphiurus, Funisciurus shown to be infected with MPXV. Here, we examine the course of MPXV infection in Cricetomys gambianus (pouched Gambian rats and this rodent species' competence as a host for the virus. We obtained ten Gambian rats from an introduced colony in Grassy Key, Florida and infected eight of these via scarification with a challenge dose of 4X104 plaque forming units (pfu from either of the two primary clades of MPXV: Congo Basin (C-MPXV: n = 4 or West African (W-MPXV: n = 4; an additional 2 animals served as PBS controls. Viral shedding and the effect of infection on activity and physiological aspects of the animals were measured. MPXV challenged animals had significantly higher core body temperatures, reduced activity and increased weight loss than PBS controls. Viable virus was found in samples taken from animals in both experimental groups (C-MPXV and W-MPXV between 3 and 27 days post infection (p.i. (up to 1X108 pfu/ml, with viral DNA found until day 56 p.i. The results from this work show that Cricetomys gambianus (and by inference, probably the closely related species, Cricetomys emini can be infected with MPXV and shed viable virus particles; thus suggesting that these animals may be involved in the maintenance of MPXV in wildlife mammalian populations. More research is needed to elucidate the epidemiology of MPXV and the role of Gambian rats and other species.

  4. Inhibition of Monkeypox virus replication by RNA interference

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    Jahrling Peter B

    2009-11-01

    Full Text Available Abstract The Orthopoxvirus genus of Poxviridae family is comprised of several human pathogens, including cowpox (CPXV, Vaccinia (VACV, monkeypox (MPV and Variola (VARV viruses. Species of this virus genus cause human diseases with various severities and outcome ranging from mild conditions to death in fulminating cases. Currently, vaccination is the only protective measure against infection with these viruses and no licensed antiviral drug therapy is available. In this study, we investigated the potential of RNA interference pathway (RNAi as a therapeutic approach for orthopox virus infections using MPV as a model. Based on genome-wide expression studies and bioinformatic analysis, we selected 12 viral genes and targeted them by small interference RNA (siRNA. Forty-eight siRNA constructs were developed and evaluated in vitro for their ability to inhibit viral replication. Two genes, each targeted with four different siRNA constructs in one pool, were limiting to viral replication. Seven siRNA constructs from these two pools, targeting either an essential gene for viral replication (A6R or an important gene in viral entry (E8L, inhibited viral replication in cell culture by 65-95% with no apparent cytotoxicity. Further analysis with wild-type and recombinant MPV expressing green fluorescence protein demonstrated that one of these constructs, siA6-a, was the most potent and inhibited viral replication for up to 7 days at a concentration of 10 nM. These results emphasis the essential role of A6R gene in viral replication, and demonstrate the potential of RNAi as a therapeutic approach for developing oligonucleotide-based drug therapy for MPV and other orthopox viruses.

  5. Experimental infection of cynomolgus macaques (Macaca fascicularis with aerosolized monkeypox virus.

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    Aysegul Nalca

    Full Text Available Monkeypox virus (MPXV infection in humans results in clinical symptoms very similar to ordinary smallpox. Aerosol is a route of secondary transmission for monkeypox, and a primary route of smallpox transmission in humans. Therefore, an animal model for aerosol exposure to MPXV is needed to test medical countermeasures. To characterize the pathogenesis in cynomolgus macaques (Macaca fascicularis, groups of macaques were exposed to four different doses of aerosolized MPXV. Blood was collected the day before, and every other day after exposure and assessed for complete blood count (CBC, clinical chemistry analysis, and quantitative PCR. Macaques showed mild anorexia, depression, and fever on day 6 post-exposure. Lymphadenopathy, which differentiates monkeypox from smallpox, was observed in exposed macaques around day 6 post-exposure. CBC and clinical chemistries showed abnormalities similar to human monkeypox cases. Whole blood and throat swab viral loads peaked around day 10, and in survivors, gradually decreased until day 28 post-exposure. Survival was not dose dependent. As such, doses of 4 × 10(4 PFU, 1 × 10(5 PFU, or 1 × 10(6 PFU resulted in lethality for 70% of the animals, whereas a dose of 4 × 10(5 PFU resulted in 85% lethality. Overall, cynomolgus macaques exposed to aerosolized MPXV develop a clinical disease that resembles that of human monkeypox. These findings provide a strong foundation for the use of aerosolized MPXV exposure of cynomolgus macaques as an animal model to test medical countermeasures against orthopoxviruses.

  6. Comparison of monkeypox viruses pathogenesis in mice by in vivo imaging.

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    Jorge E Osorio

    Full Text Available Monkeypox viruses (MPXV cause human monkeypox, a zoonotic smallpox-like disease endemic to Africa, and are of worldwide public health and biodefense concern. Using viruses from the Congo (MPXV-2003-Congo-358 and West African (MPXV-2003-USA-044 clades, we constructed recombinant viruses that express the luciferase gene (MPXV-Congo/Luc+and MPXV-USA-Luc+ and compared their viral infection in mice by biophotonic imaging. BALB/c mice became infected by both MPXV clades, but they recovered and cleared the infection within 10 days post-infection (PI. However, infection in severe combined immune deficient (SCID BALB/c mice resulted in 100% lethality. Intraperitoneal (IP injection of both MPXV-Congo and MPXV-Congo/Luc+resulted in a systemic clinical disease and the same mean time-to-death at 9 (+/-0 days post-infection. Likewise, IP injection of SCID-BALB/c mice with MPXV-USA or the MPXV-USA-Luc+, resulted in similar disease but longer (P<0.05 mean time-to-death (11+/-0 days for both viruses compared to the Congo strains. Imaging studies in SCID mice showed luminescence in the abdomen within 24 hours PI with subsequent spread elsewhere. Animals infected with the MPXV-USA/Luc+had less intense luminescence in tissues than those inoculated with MPXV-Congo/Luc+, and systemic spread of the MPXV-USA/Luc+virus occurred approximately two days later than the MPXV-Congo/Luc+. The ovary was an important target for viral replication as evidenced by the high viral titers and immunohistochemistry. These studies demonstrate the suitability of a mouse model and biophotonic imaging to compare the disease progression and tissue tropism of MPX viruses.

  7. Proteomic basis of the antibody response to monkeypox virus infection examined in cynomolgus macaques and a comparison to human smallpox vaccination.

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    Sarah Keasey

    Full Text Available Monkeypox is a zoonotic viral disease that occurs primarily in Central and West Africa. A recent outbreak in the United States heightened public health concerns for susceptible human populations. Vaccinating with vaccinia virus to prevent smallpox is also effective for monkeypox due to a high degree of sequence conservation. Yet, the identity of antigens within the monkeypox virus proteome contributing to immune responses has not been described in detail. We compared antibody responses to monkeypox virus infection and human smallpox vaccination by using a protein microarray covering 92-95% (166-192 proteins of representative proteomes from monkeypox viral clades of Central and West Africa, including 92% coverage (250 proteins of the vaccinia virus proteome as a reference orthopox vaccine. All viral gene clones were verified by sequencing and purified recombinant proteins were used to construct the microarray. Serum IgG of cynomolgus macaques that recovered from monkeypox recognized at least 23 separate proteins within the orthopox proteome, while only 14 of these proteins were recognized by IgG from vaccinated humans. There were 12 of 14 antigens detected by sera of human vaccinees that were also recognized by IgG from convalescent macaques. The greatest level of IgG binding for macaques occurred with the structural proteins F13L and A33R, and the membrane scaffold protein D13L. Significant IgM responses directed towards A44R, F13L and A33R of monkeypox virus were detected before onset of clinical symptoms in macaques. Thus, antibodies from vaccination recognized a small number of proteins shared with pathogenic virus strains, while recovery from infection also involved humoral responses to antigens uniquely recognized within the monkeypox virus proteome.

  8. Evaluation of monkeypox virus infection of prairie dogs (Cynomys ludovicianus) using in vivo bioluminescent imaging

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    Falendysz, Elizabeth A.; Londoño-Navas, Angela M.; Meteyer, Carol U.; Pussini, Nicola; Lopera, Juan G.; Osorio, Jorge E.; Rocke, Tonie E.

    2014-01-01

    Monkeypox (MPX) is a re-emerging zoonotic disease that is endemic in Central and West Africa, where it can cause a smallpox-like disease in humans. Despite many epidemiologic and field investigations of MPX, no definitive reservoir species has been identified. Using recombinant viruses expressing the firefly luciferase (luc) gene, we previously demonstrated the suitability of in vivo bioluminescent imaging (BLI) to study the pathogenesis of MPX in animal models. Here, we evaluated BLI as a novel approach for tracking MPX virus infection in black-tailed prairie dogs (Cynomys ludovicianus). Prairie dogs were affected during a multistate outbreak of MPX in the US in 2003 and have since been used as an animal model of this disease. Our BLI results were compared with PCR and virus isolation from tissues collected postmortem. Virus was easily detected and quantified in skin and superficial tissues by BLI before and during clinical phases, as well as in subclinical secondary cases, but was not reliably detected in deep tissues such as the lung. Although there are limitations to viral detection in larger wild rodent species, BLI can enhance the use of prairie dogs as an animal model of MPX and can be used for the study of infection, disease progression, and transmission in potential wild rodent reservoirs.

  9. EVALUATION OF MONKEYPOX VIRUS INFECTION OF BLACK-TAILED PRAIRIE DOGS (CYNOMYS LUDOVICIANUS) USING IN VIVO BIOLUMINESCENT IMAGING

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    Falendysz, Elizabeth A.; Londoño-Navas, Angela M.; Carol U Meteyer; Pussini, Nicola; Lopera, Juan G.; Osorio, Jorge E.; Tonie E Rocke

    2014-01-01

    Monkeypox (MPX) is a re-emerging zoonotic disease that is endemic in Central and West Africa, where it can cause a smallpox-like disease in humans. Despite many epidemiologic and field investigations of MPX, no definitive reservoir species has been identified. Using recombinant viruses expressing the firefly luciferase (luc) gene, we previously demonstrated the suitability of in vivo bioluminescent imaging (BLI) to study the pathogenesis of MPX in animal models. Here, we evaluated BLI as a no...

  10. Stunned silence: gene expression programs in human cells infected with monkeypox or vaccinia virus.

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    Kathleen H Rubins

    Full Text Available Poxviruses use an arsenal of molecular weapons to evade detection and disarm host immune responses. We used DNA microarrays to investigate the gene expression responses to infection by monkeypox virus (MPV, an emerging human pathogen, and Vaccinia virus (VAC, a widely used model and vaccine organism, in primary human macrophages, primary human fibroblasts and HeLa cells. Even as the overwhelmingly infected cells approached their demise, with extensive cytopathic changes, their gene expression programs appeared almost oblivious to poxvirus infection. Although killed (gamma-irradiated MPV potently induced a transcriptional program characteristic of the interferon response, no such response was observed during infection with either live MPV or VAC. Moreover, while the gene expression response of infected cells to stimulation with ionomycin plus phorbol 12-myristate 13-acetate (PMA, or poly (I-C was largely unimpaired by infection with MPV, a cluster of pro-inflammatory genes were a notable exception. Poly(I-C induction of genes involved in alerting the innate immune system to the infectious threat, including TNF-alpha, IL-1 alpha and beta, CCL5 and IL-6, were suppressed by infection with live MPV. Thus, MPV selectively inhibits expression of genes with critical roles in cell-signaling pathways that activate innate immune responses, as part of its strategy for stealthy infection.

  11. Further assessment of Monkeypox Virus infection in Gambian pouched rats (Cricetomys gambianus) using in vivo bioluminescent imaging

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    Falendysz, Elizabeth; Lopera, Juan G.; Faye Lorenzsonn,; Salzer, Johanna S.; Hutson, Christina L.; Doty, Jeffrey; Gallardo-Romero, Nadia; Carroll, Darin S.; Osorio, Jorge E.; Rocke, Tonie E.

    2015-01-01

    Monkeypox is a zoonosis clinically similar to smallpox in humans. Recent evidence has shown a potential risk of increased incidence in central Africa. Despite attempts to isolate the virus from wild rodents and other small mammals, no reservoir host has been identified. In 2003,Monkeypox virus (MPXV) was accidentally introduced into the U.S. via the pet trade and was associated with the Gambian pouched rat (Cricetomys gambianus). Therefore, we investigated the potential reservoir competence of the Gambian pouched rat for MPXV by utilizing a combination of in vivo and in vitro methods. We inoculated three animals by the intradermal route and three animals by the intranasal route, with one mock-infected control for each route. Bioluminescent imaging (BLI) was used to track replicating virus in infected animals and virological assays (e.g. real time PCR, cell culture) were used to determine viral load in blood, urine, ocular, nasal, oral, and rectal swabs. Intradermal inoculation resulted in clinical signs of monkeypox infection in two of three animals. One severely ill animal was euthanized and the other affected animal recovered. In contrast, intranasal inoculation resulted in subclinical infection in all three animals. All animals, regardless of apparent or inapparent infection, shed virus in oral and nasal secretions. Additionally, BLI identified viral replication in the skin without grossly visible lesions. These results suggest that Gambian pouched rats may play an important role in transmission of the virus to humans, as they are hunted for consumption and it is possible for MPXV-infected pouched rats to shed infectious virus without displaying overt clinical signs.

  12. Further Assessment of Monkeypox Virus Infection in Gambian Pouched Rats (Cricetomys gambianus) Using In Vivo Bioluminescent Imaging.

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    Falendysz, Elizabeth A; Lopera, Juan G; Lorenzsonn, Faye; Salzer, Johanna S; Hutson, Christina L; Doty, Jeffrey; Gallardo-Romero, Nadia; Carroll, Darin S; Osorio, Jorge E; Rocke, Tonie E

    2015-01-01

    Monkeypox is a zoonosis clinically similar to smallpox in humans. Recent evidence has shown a potential risk of increased incidence in central Africa. Despite attempts to isolate the virus from wild rodents and other small mammals, no reservoir host has been identified. In 2003, Monkeypox virus (MPXV) was accidentally introduced into the U.S. via the pet trade and was associated with the Gambian pouched rat (Cricetomys gambianus). Therefore, we investigated the potential reservoir competence of the Gambian pouched rat for MPXV by utilizing a combination of in vivo and in vitro methods. We inoculated three animals by the intradermal route and three animals by the intranasal route, with one mock-infected control for each route. Bioluminescent imaging (BLI) was used to track replicating virus in infected animals and virological assays (e.g. real time PCR, cell culture) were used to determine viral load in blood, urine, ocular, nasal, oral, and rectal swabs. Intradermal inoculation resulted in clinical signs of monkeypox infection in two of three animals. One severely ill animal was euthanized and the other affected animal recovered. In contrast, intranasal inoculation resulted in subclinical infection in all three animals. All animals, regardless of apparent or inapparent infection, shed virus in oral and nasal secretions. Additionally, BLI identified viral replication in the skin without grossly visible lesions. These results suggest that Gambian pouched rats may play an important role in transmission of the virus to humans, as they are hunted for consumption and it is possible for MPXV-infected pouched rats to shed infectious virus without displaying overt clinical signs. PMID:26517839

  13. Treatment with the smallpox antiviral tecovirimat (ST-246) alone or in combination with ACAM2000 vaccination is effective as a postsymptomatic therapy for monkeypox virus infection.

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    Berhanu, Aklile; Prigge, Jonathan T; Silvera, Peter M; Honeychurch, Kady M; Hruby, Dennis E; Grosenbach, Douglas W

    2015-07-01

    The therapeutic efficacies of smallpox vaccine ACAM2000 and antiviral tecovirimat given alone or in combination starting on day 3 postinfection were compared in a cynomolgus macaque model of lethal monkeypox virus infection. Postexposure administration of ACAM2000 alone did not provide any protection against severe monkeypox disease or mortality. In contrast, postexposure treatment with tecovirimat alone or in combination with ACAM2000 provided full protection. Additionally, tecovirimat treatment delayed until day 4, 5, or 6 postinfection was 83% (days 4 and 5) or 50% (day 6) effective.

  14. Modified vaccinia virus Ankara protects macaques against respiratory challenge with monkeypox virus

    NARCIS (Netherlands)

    Stittelaar, Koert J; van Amerongen, Geert; Kondova, Ivanela; Kuiken, Thijs; van Lavieren, Rob F; Pistoor, Frank H M; Niesters, Hubert G M; van Doornum, Gerard; van der Zeijst, Ben A M; Mateo, Luis; Chaplin, Paul J; Osterhaus, Albert D M E

    2005-01-01

    The use of classical smallpox vaccines based on vaccinia virus (VV) is associated with severe complications in both naive and immune individuals. Modified vaccinia virus Ankara (MVA), a highly attenuated replication-deficient strain of VV, has been proven to be safe in humans and immunocompromised a

  15. Modified vaccinia virus Ankara protects macaques against respiratory challenge with monkeypox virus.

    NARCIS (Netherlands)

    K.J. Stittelaar (Koert); G. van Amerongen (Geert); I. Kondova (Ivanela); R.F. van Lavieren (Rob); F.H. Pistoor (Frank); H.G.M. Niesters (Bert); G.J.J. van Doornum (Gerard); B.A.M. van der Zeijst (Ben); L. Mateo (Luis); P.J. Chaplin (Paul); A.D.M.E. Osterhaus (Albert); T. Kuiken (Thijs)

    2005-01-01

    textabstractThe use of classical smallpox vaccines based on vaccinia virus (VV) is associated with severe complications in both naive and immune individuals. Modified vaccinia virus Ankara (MVA), a highly attenuated replication-deficient strain of VV, has been proven to be safe in humans and immunoc

  16. Evaluation of monkeypox virus infection of black-tailed prairie dogs (Cynomys ludovicianus) using in vivo bioluminescent imaging.

    Science.gov (United States)

    Falendysz, Elizabeth A; Londoño-Navas, Angela M; Meteyer, Carol U; Pussini, Nicola; Lopera, Juan G; Osorio, Jorge E; Rocke, Tonie E

    2014-07-01

    Monkeypox (MPX) is a re-emerging zoonotic disease that is endemic in Central and West Africa, where it can cause a smallpox-like disease in humans. Despite many epidemiologic and field investigations of MPX, no definitive reservoir species has been identified. Using recombinant viruses expressing the firefly luciferase (luc) gene, we previously demonstrated the suitability of in vivo bioluminescent imaging (BLI) to study the pathogenesis of MPX in animal models. Here, we evaluated BLI as a novel approach for tracking MPX virus infection in black-tailed prairie dogs (Cynomys ludovicianus). Prairie dogs were affected during a multistate outbreak of MPX in the US in 2003 and have since been used as an animal model of this disease. Our BLI results were compared with PCR and virus isolation from tissues collected postmortem. Virus was easily detected and quantified in skin and superficial tissues by BLI before and during clinical phases, as well as in subclinical secondary cases, but was not reliably detected in deep tissues such as the lung. Although there are limitations to viral detection in larger wild rodent species, BLI can enhance the use of prairie dogs as an animal model of MPX and can be used for the study of infection, disease progression, and transmission in potential wild rodent reservoirs. PMID:24779460

  17. Outbreaks of human monkeypox after cessation of smallpox vaccination.

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    Reynolds, Mary G; Damon, Inger K

    2012-02-01

    The recent observation of a surge in human monkeypox in the Democratic Republic of the Congo (DRC) prompts the question of whether cessation of smallpox vaccination is driving the phenomenon, and if so, why is re-emergence not universal throughout the historic geographic range of the virus? Research addressing the virus's mechanisms for immune evasion and induction, as well as that directed at elucidating the genes involved in pathogenesis in different viral lineages (West African vs Congo Basin), provide insights to help explain why emergence appears to be geographically limited. Novel vaccines offer one solution to curtail the spread of this disease.

  18. Ecological niche and geographic distribution of human monkeypox in Africa.

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    Rebecca S Levine

    Full Text Available Monkeypox virus, a zoonotic member of the genus Orthopoxviridae, can cause a severe, smallpox-like illness in humans. Monkeypox virus is thought to be endemic to forested areas of western and Central Africa. Considerably more is known about human monkeypox disease occurrence than about natural sylvatic cycles of this virus in non-human animal hosts. We use human monkeypox case data from Africa for 1970-2003 in an ecological niche modeling framework to construct predictive models of the ecological requirements and geographic distribution of monkeypox virus across West and Central Africa. Tests of internal predictive ability using different subsets of input data show the model to be highly robust and suggest that the distinct phylogenetic lineages of monkeypox in West Africa and Central Africa occupy similar ecological niches. High mean annual precipitation and low elevations were shown to be highly correlated with human monkeypox disease occurrence. The synthetic picture of the potential geographic distribution of human monkeypox in Africa resulting from this study should support ongoing epidemiologic and ecological studies, as well as help to guide public health intervention strategies to areas at highest risk for human monkeypox.

  19. Monkeypox virus infection of rhesus macaques induces massive expansion of natural killer cells but suppresses natural killer cell functions.

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    Haifeng Song

    Full Text Available Natural killer (NK cells play critical roles in innate immunity and in bridging innate and adaptive immune responses against viral infection. However, the response of NK cells to monkeypox virus (MPXV infection is not well characterized. In this intravenous challenge study of MPXV infection in rhesus macaques (Macaca mulatta, we analyzed blood and lymph node NK cell changes in absolute cell numbers, cell proliferation, chemokine receptor expression, and cellular functions. Our results showed that the absolute number of total NK cells in the blood increased in response to MPXV infection at a magnitude of 23-fold, manifested by increases in CD56+, CD16+, CD16-CD56- double negative, and CD16+CD56+ double positive NK cell subsets. Similarly, the frequency and NK cell numbers in the lymph nodes also largely increased with the total NK cell number increasing 46.1-fold. NK cells both in the blood and lymph nodes massively proliferated in response to MPXV infection as measured by Ki67 expression. Chemokine receptor analysis revealed reduced expression of CXCR3, CCR7, and CCR6 on NK cells at early time points (days 2 and 4 after virus inoculation, followed by an increased expression of CXCR3 and CCR5 at later time points (days 7-8 of infection. In addition, MPXV infection impaired NK cell degranulation and ablated secretion of interferon-γ and tumor necrosis factor-α. Our data suggest a dynamic model by which NK cells respond to MPXV infection of rhesus macaques. Upon virus infection, NK cells proliferated robustly, resulting in massive increases in NK cell numbers. However, the migrating capacity of NK cells to tissues at early time points might be reduced, and the functions of cytotoxicity and cytokine secretion were largely compromised. Collectively, the data may explain, at least partially, the pathogenesis of MPXV infection in rhesus macaques.

  20. Establishment of real-time PCR assays for detection of monkeypox virus%猴痘病毒荧光定量PCR检测方法的建立

    Institute of Scientific and Technical Information of China (English)

    朱娜; 陈国强; 张敬友; 范红结

    2009-01-01

    根据GenBank发表的猴痘病毒(AF380138)F3L基因全序列,设计并合成了引物和TaqMan、MGB探针,建立了F3L基因的荧光定量PCR检测方法.应用该方法可从人工合成的猴痘病毒F3L基因片段(48048~48 509 bp)扩增典型的"S"型曲线,25 μL反应体系检测灵敏度可达68拷贝,但不能从鸡痘、山羊痘等病毒中扩增出相应的扩增曲线.结论:本方法可快速、敏感、特异地检测猴痘病毒.%In this paper the primers and TaqMan, MGB probes were designed by using Primer Express 3. 0 software according to the entire F3L gene sequence of monkeypox virus in CenBank to develop a real-time PCR assay for rapid identification of monkeypox virus. A typical "S" curve can be got when the artifical F3L gene sequence was used as a target, and the detection limits were 6. 8 copies of target DNA per PCR vial. However, when the chickenpox, goalpox or any other virus were used as targets, a typical " S" curve can not be got. So this assay may represent a battery of tests in detection of monkeypox virus.

  1. Antiviral treatment is more effective than smallpox vaccination upon lethal monkeypox virus infection

    NARCIS (Netherlands)

    Stittelaar, Koert J; Neyts, Johan; Naesens, Lieve; van Amerongen, Geert; van Lavieren, Rob F; Holý, Antonin; De Clercq, Erik; Niesters, Hubert G M; Fries, Edwin; Maas, Chantal; Mulder, Paul G H; van der Zeijst, Ben A M; Osterhaus, Albert D M E

    2006-01-01

    There is concern that variola virus, the aetiological agent of smallpox, may be used as a biological weapon. For this reason several countries are now stockpiling (vaccinia virus-based) smallpox vaccine. Although the preventive use of smallpox vaccination has been well documented, little is known ab

  2. Pathogen-host associations and predicted range shifts of human monkeypox in response to climate change in central Africa.

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    Henri A Thomassen

    Full Text Available Climate change is predicted to result in changes in the geographic ranges and local prevalence of infectious diseases, either through direct effects on the pathogen, or indirectly through range shifts in vector and reservoir species. To better understand the occurrence of monkeypox virus (MPXV, an emerging Orthopoxvirus in humans, under contemporary and future climate conditions, we used ecological niche modeling techniques in conjunction with climate and remote-sensing variables. We first created spatially explicit probability distributions of its candidate reservoir species in Africa's Congo Basin. Reservoir species distributions were subsequently used to model current and projected future distributions of human monkeypox (MPX. Results indicate that forest clearing and climate are significant driving factors of the transmission of MPX from wildlife to humans under current climate conditions. Models under contemporary climate conditions performed well, as indicated by high values for the area under the receiver operator curve (AUC, and tests on spatially randomly and non-randomly omitted test data. Future projections were made on IPCC 4(th Assessment climate change scenarios for 2050 and 2080, ranging from more conservative to more aggressive, and representing the potential variation within which range shifts can be expected to occur. Future projections showed range shifts into regions where MPX has not been recorded previously. Increased suitability for MPX was predicted in eastern Democratic Republic of Congo. Models developed here are useful for identifying areas where environmental conditions may become more suitable for human MPX; targeting candidate reservoir species for future screening efforts; and prioritizing regions for future MPX surveillance efforts.

  3. Spectrum of Infection and Risk Factors for Human Monkeypox, United States, 2003

    OpenAIRE

    Mary G. Reynolds; Davidson, Whitni B.; Curns, Aaron T.; Conover, Craig S.; Huhn, Gregory; Davis, Jeffrey P.; Wegner, Mark; Croft, Donita R.; Newman, Alexandra; Obiesie, Nkolika N.; Hansen, Gail R.; Hays, Patrick L.; Pontones, Pamela; Beard, Brad; Teclaw, Robert

    2007-01-01

    For the 2003 monkeypox virus (MPXV) outbreak in the United States, interhuman transmission was not documented and all case-patients were near or handled MPXV-infected prairie dogs. We initiated a case–control study to evaluate risk factors for animal-to-human MPXV transmission. Participants completed a questionnaire requesting exposure, clinical, and demographic information. Serum samples were obtained for analysis of immunoglobulin G (IgG) and IgM to orthopoxvirus. When data were adjusted fo...

  4. Monkeypox disease transmission in an experimental setting: prairie dog animal model.

    Directory of Open Access Journals (Sweden)

    Christina L Hutson

    Full Text Available Monkeypox virus (MPXV is considered the most significant human public health threat in the genus Orthopoxvirus since the eradication of variola virus (the causative agent of smallpox. MPXV is a zoonotic agent endemic to forested areas of Central and Western Africa. In 2003, MPXV caused an outbreak in the United States due to the importation of infected African rodents, and subsequent sequential infection of North American prairie dogs (Cynomys ludovicianus and humans. In previous studies, the prairie dog MPXV model has successfully shown to be very useful for understanding MPXV since the model emulates key characteristics of human monkeypox disease. In humans, percutaneous exposure to animals has been documented but the primary method of human-to-human MPXV transmission is postulated to be by respiratory route. Only a few animal model studies of MPXV transmission have been reported. Herein, we show that MPXV infected prairie dogs are able to transmit the virus to naive animals through multiple transmission routes. All secondarily exposed animals were infected with MPXV during the course of the study. Notably, animals secondarily exposed appeared to manifest more severe disease; however, the disease course was very similar to those of experimentally challenged animals including inappetence leading to weight loss, development of lesions, production of orthopoxvirus antibodies and shedding of similar levels or in some instances higher levels of MPXV from the oral cavity. Disease was transmitted via exposure to contaminated bedding, co-housing, or respiratory secretions/nasal mucous (we could not definitively say that transmission occurred via respiratory route exclusively. Future use of the model will allow us to evaluate infection control measures, vaccines and antiviral strategies to decrease disease transmission.

  5. Effects of georeferencing effort on mapping monkeypox case distributions and transmission risk

    Directory of Open Access Journals (Sweden)

    Lash R

    2012-06-01

    Full Text Available Abstract Background Maps of disease occurrences and GIS-based models of disease transmission risk are increasingly common, and both rely on georeferenced diseases data. Automated methods for georeferencing disease data have been widely studied for developed countries with rich sources of geographic referenced data. However, the transferability of these methods to countries without comparable geographic reference data, particularly when working with historical disease data, has not been as widely studied. Historically, precise geographic information about where individual cases occur has been collected and stored verbally, identifying specific locations using place names. Georeferencing historic data is challenging however, because it is difficult to find appropriate geographic reference data to match the place names to. Here, we assess the degree of care and research invested in converting textual descriptions of disease occurrence locations to numerical grid coordinates (latitude and longitude. Specifically, we develop three datasets from the same, original monkeypox disease occurrence data, with varying levels of care and effort: the first based on an automated web-service, the second improving on the first by reference to additional maps and digital gazetteers, and the third improving still more based on extensive consultation of legacy surveillance records that provided considerable additional information about each case. To illustrate the implications of these seemingly subtle improvements in data quality, we develop ecological niche models and predictive maps of monkeypox transmission risk based on each of the three occurrence data sets. Results We found macrogeographic variations in ecological niche models depending on the type of georeferencing method used. Less-careful georeferencing identified much smaller areas as having potential for monkeypox transmission in the Sahel region, as well as around the rim of the Congo Basin. These

  6. Development of a model describing virus removal process in an activated sludge basin

    Energy Technology Data Exchange (ETDEWEB)

    Kim, T.; Shiragami, N. Unno, H. [Tokyo Institute of Technology, Tokyo (Japan)

    1995-06-20

    The virus removal process from the liquid phase in an activated sludge basin possibly consists of physicochemical processes, such as adsorption onto sludge flocs, biological processes such as microbial predating and inactivation by virucidal components excreted by microbes. To describe properly the virus behavior in an activated sludge basin, a simple model is proposed based on the experimental data obtained using a poliovirus type 1. A three-compartments model, which include the virus in the liquid phase and in the peripheral and inner regions of sludge flocs is employed. By using the model, the Virus removal process was successfully simulated to highlight the implication of its distribution in the activated sludge basin. 17 refs., 8 figs.

  7. Isolation of viruses from mosquitoes (Diptera: Culicidae) collected in the Amazon Basin region of Peru.

    Science.gov (United States)

    Turell, M J; O'Guinn, M L; Jones, J W; Sardelis, M R; Dohm, D J; Watts, D M; Fernandez, R; Travassos da Rosa, A; Guzman, H; Tesh, R; Rossi, C A; Ludwig, V; Mangiafico, J A; Kondig, J; Wasieloski, L P; Pecor, J; Zyzak, M; Schoeler, G; Mores, C N; Calampa, C; Lee, J S; Klein, T A

    2005-09-01

    As part of a comprehensive study on the ecology of arthropod-borne viruses in the Amazon Basin region of Peru, we assayed 539,694 mosquitoes captured in Loreto Department, Peru, for arboviruses. Mosquitoes were captured either by dry ice-baited miniature light traps or with aspirators while mosquitoes were landing on human collectors, identified to species, and later tested on Vero cells for virus. In total, 164 virus isolations were made and included members of the Alphavirus (eastern equine encephalomyelitis, Trocara, Una, Venezuelan equine encephalomyelitis, and western equine encephalomyelitis viruses), Flavivirus (Ilheus and St. Louis encephalitis), and Orthobunyavirus (Caraparu, Itaqui, Mirim, Murutucu, and Wyeomyia viruses) genera. In addition, several viruses distinct from the above-mentioned genera were identified to the serogroup level. Eastern equine encephalomyelitis virus was associated primarily with Culex pedroi Sirivanakarn & Belkin, whereas Venezuelan equine encephalomyelitis virus was associated primarily with Culex gnomatos Sallum, Huchings & Ferreira. Most isolations of Ilheus virus were made from Psorophora ferox (Von Humboldt). Although species of the Culex subgenus Melanoconion accounted for only 45% of the mosquitoes collected, 85% of the virus isolations were made from this subgenus. Knowledge of the viruses that are being transmitted in the Amazon Basin region of Peru will enable the development of more effective diagnostic assays, more efficient and rapid diagnoses of clinical illnesses caused by these pathogens, risk analysis for military/civilian operations, and development of potential disease control measures.

  8. Mayaro virus infection, Amazon Basin region, Peru, 2010-2013.

    Science.gov (United States)

    Halsey, Eric S; Siles, Crystyan; Guevara, Carolina; Vilcarromero, Stalin; Jhonston, Erik J; Ramal, Cesar; Aguilar, Patricia V; Ampuero, Julia S

    2013-11-01

    During 2010-2013, we recruited 16 persons with confirmed Mayaro virus infection in the Peruvian Amazon to prospectively follow clinical symptoms and serologic response over a 12-month period. Mayaro virus infection caused long-term arthralgia in more than half, similar to reports of other arthritogenic alphaviruses.

  9. Viruses Surveillance Under Different Season Scenarios of the Negro River Basin, Amazonia, Brazil.

    Science.gov (United States)

    Vieira, Carmen Baur; de Abreu Corrêa, Adriana; de Jesus, Michele Silva; Luz, Sérgio Luiz Bessa; Wyn-Jones, Peter; Kay, David; Vargha, Marta; Miagostovich, Marize Pereira

    2016-03-01

    The Negro River is located in the Amazon basin, the largest hydrological catchment in the world. Its water is used for drinking, domestic activities, recreation and transportation and water quality is significantly affected by anthropogenic impacts. The goals of this study were to determine the presence and concentrations of the main viral etiological agents of acute gastroenteritis, such as group A rotavirus (RVA) and genogroup II norovirus (NoV GII), and to assess the use of human adenovirus (HAdV) and JC polyomavirus (JCPyV) as viral indicators of human faecal contamination in the aquatic environment of Manaus under different hydrological scenarios. Water samples were collected along Negro River and in small streams known as igarapés. Viruses were concentrated by an organic flocculation method and detected by quantitative PCR. From 272 samples analysed, HAdV was detected in 91.9%, followed by JCPyV (69.5%), RVA (23.9%) and NoV GII (7.4%). Viral concentrations ranged from 10(2) to 10(6) GC L(-1) and viruses were more likely to be detected during the flood season, with the exception of NoV GII, which was detected only during the dry season. Statistically significant differences on virus concentrations between dry and flood seasons were observed only for RVA. The HAdV data provides a useful complement to faecal indicator bacteria in the monitoring of aquatic environments. Overall results demonstrated that the hydrological cycle of the Negro River in the Amazon Basin affects the dynamics of viruses in aquatic environments and, consequently, the exposure of citizens to these waterborne pathogens.

  10. Pepino mosaic virus and Tomato torrado virus: two emerging viruses affecting tomato crops in the Mediterranean basin.

    Science.gov (United States)

    Gómez, Pedro; Sempere, Raqueln; Aranda, Miguel A

    2012-01-01

    The molecular biology, epidemiology, and evolutionary dynamics of Pepino mosaic virus (PepMV) are much better understood than those of Tomato torrado virus (ToTV). The earliest descriptions of PepMV suggest a recent jump from nontomato species (e.g., pepino; Solanum muricatum) to tomato (Solanum lycopersicum). Its stability in contaminated plant tissues, its transmission through seeds, and the global trade of tomato seeds and fruits may have facilitated the global spread of PepMV. Stability and seed transmission also probably account for the devastating epidemics caused by already-established PepMV strains, although additional contributing factors may include the efficient transmission of PepMV by contact and the often-inconspicuous symptoms in vegetative tomato tissues. The genetic variability of PepMV is likely to have promoted the first phase of emergence (i.e., the species jump) and it continues to play an important role as the virus becomes more pervasive, progressing from regional outbreaks to pandemics. In contrast, the long-term progression of ToTV outbreaks is not yet clear and this may reflect factors such as the limited accumulation of the virus in infected plants, which has been shown to be approximately two orders of magnitude less than PepMV. The efficient dispersion of ToTV may therefore depend on dense populations of its principal vectors, Bemisia tabaci and Trialeurodes vaporariorum, as has been proposed for the necrogenic satellite RNA of Cucumber mosaic virus.

  11. Predictive modeling of West Nile virus transmission risk in the Mediterranean Basin: how far from landing?

    Science.gov (United States)

    Chevalier, Véronique; Tran, Annelise; Durand, Benoit

    2014-01-01

    The impact on human and horse health of West Nile fever (WNF) recently and dramatically increased in Europe and neighboring countries. Involving several mosquito and wild bird species, WNF epidemiology is complex. Despite the implementation of surveillance systems in several countries of concern, and due to a lack of knowledge, outbreak occurrence remains unpredictable. Statistical models may help identifying transmission risk factors. When spatialized, they provide tools to identify areas that are suitable for West Nile virus transmission. Mathematical models may be used to improve our understanding of epidemiological process involved, to evaluate the impact of environmental changes or test the efficiency of control measures. We propose a systematic literature review of publications aiming at modeling the processes involved in WNF transmission in the Mediterranean Basin. The relevance of the corresponding models as predictive tools for risk mapping, early warning and for the design of surveillance systems in a changing environment is analyzed. PMID:24362544

  12. Predictive Modeling of West Nile Virus Transmission Risk in the Mediterranean Basin: How Far from Landing?

    Directory of Open Access Journals (Sweden)

    Véronique Chevalier

    2013-12-01

    Full Text Available The impact on human and horse health of West Nile fever (WNF recently and dramatically increased in Europe and neighboring countries. Involving several mosquito and wild bird species, WNF epidemiology is complex. Despite the implementation of surveillance systems in several countries of concern, and due to a lack of knowledge, outbreak occurrence remains unpredictable. Statistical models may help identifying transmission risk factors. When spatialized, they provide tools to identify areas that are suitable for West Nile virus transmission. Mathematical models may be used to improve our understanding of epidemiological process involved, to evaluate the impact of environmental changes or test the efficiency of control measures. We propose a systematic literature review of publications aiming at modeling the processes involved in WNF transmission in the Mediterranean Basin. The relevance of the corresponding models as predictive tools for risk mapping, early warning and for the design of surveillance systems in a changing environment is analyzed.

  13. 42 CFR 71.56 - African rodents and other animals that may carry the monkeypox virus.

    Science.gov (United States)

    2010-10-01

    ... HUMAN SERVICES QUARANTINE, INSPECTION, LICENSING FOREIGN QUARANTINE Importations § 71.56 African rodents... Control and Prevention (CDC) from causing an animal to be quarantined, re-exported, or destroyed under a... obtain such written permission from CDC, you must send a written request to Division of Global...

  14. Monkeypox Virus Infections in Small Animal Models for Evaluation of Anti-Poxvirus Agents

    OpenAIRE

    Inger K Damon; Christina L Hutson

    2010-01-01

    An ideal animal model for the study of a human disease is one which utilizes a route of infection that mimics the natural transmission of the pathogen; the ability to obtain disease with an infectious dose equivalent to that causing disease in humans; as well having a disease course, morbidity and mortality similar to that seen with human disease. Additionally, the animal model should have a mode(s) of transmission that mimics human cases. The development of small animal models for the study ...

  15. A Multicenter, Open-label Study of CMX001 Treatment of Serious Diseases or Conditions Caused by dsDNA Viruses

    Science.gov (United States)

    2013-08-28

    Male or Female Patients With a Serious or Immediately Life-threatening; Disease or Condition Caused by CMV, ADV, HSV, VAVC, VARV or; Monkeypox Viruses(s) Who Have a Life Expectancy of ≥ 2 Weeks and for; Whom no Comparable or Satisfactory Alternative Therapy is Available

  16. Spatial and temporal dynamics of hepatitis B virus D genotype in Europe and the Mediterranean Basin.

    Directory of Open Access Journals (Sweden)

    Gianguglielmo Zehender

    Full Text Available Hepatitis B virus genotype D can be found in many parts of the world and is the most prevalent strain in south-eastern Europe, the Mediterranean Basin, the Middle East, and the Indian sub-continent. The epidemiological history of the D genotype and its subgenotypes is still obscure because of the scarcity of appropriate studies. We retrieved from public databases a total of 312 gene P sequences of HBV genotype D isolated in various countries throughout the world, and reconstructed the spatio-temporal evolutionary dynamics of the HBV-D epidemic using a bayesian framework.The phylogeographical analysis showed that India had the highest posterior probability of being the location of the tree root, whereas central Asia was the most probable location of the common ancestor of subgenotypes D1-D3. HBV-D5 (identified in native Indian populations diverged from the tree root earlier than D1-D3. The time of the most recent common ancestor (tMRCA of the tree root was 128 years ago, which suggests that the common ancestor of the currently circulating subgenotypes existed in the second half of the XIX century. The mean tMRCA of subgenotypes D1-D3 was between the 1940s and the 1950-60s. On the basis of our phylogeographic reconstruction, it seems that HBV-D reached the Mediterranean area in the middle of the XX century by means of at least two routes: the first pathway (mainly due to the spread of subgenotype D1 crossing the Middle East and reaching north Africa and the eastern Mediterranean, and the second pathway (closely associated with D2 that crossed the former Soviet Union and reached eastern Europe and the Mediterranean through Albania. We hypothesise that the main route of dispersion of genotype D was the unsafe use of injections and drug addiction.

  17. Genetic analysis of South American eastern equine encephalomyelitis viruses isolated from mosquitoes collected in the Amazon Basin region of Peru.

    Science.gov (United States)

    Kondig, John P; Turell, Michael J; Lee, John S; O'Guinn, Monica L; Wasieloski, Leonard P

    2007-03-01

    Identifying viral isolates from field-collected mosquitoes can be difficult and time-consuming, particularly in regions of the world where numerous closely related viruses are co-circulating (e.g., the Amazon Basin region of Peru). The use of molecular techniques may provide rapid and efficient methods for identifying these viruses in the laboratory. Therefore, we determined the complete nucleotide sequence of two South American eastern equine encephalomyelitis viruses (EEEVs): one member from the Peru-Brazil (Lineage II) clade and one member from the Argentina-Panama (Lineage III) clade. In addition, we determined the nucleotide sequence for the nonstructural P3 protein (nsP3) and envelope 2 (E2) protein genes of 36 additional isolates of EEEV from mosquitoes captured in Peru between 1996 and 2001. The 38 isolates were evenly distributed between lineages II and III virus groupings. However, analysis of the nsP3 gene for lineage III strongly suggested that the 19 isolates from this lineage could be divided into two sub-clades, designated as lineages III and IIIA. Compared with North American EEEV (lineage I, GA97 strain), we found that the length of the nsP3 gene was shorter in the strains isolated from South America. A total of 60 nucleotides was deleted in lineage II, 69 in lineage III, and 72 in lineage IIIA. On the basis of the sequences we determined for South American EEEVs and those for other viruses detected in the same area, we developed a series of primers for characterizing these viruses.

  18. Circulation of a Meaban-like virus in yellow-legged gulls and seabird ticks in the western Mediterranean basin.

    Directory of Open Access Journals (Sweden)

    Audrey Arnal

    Full Text Available In recent years, a number of zoonotic flaviviruses have emerged worldwide, and wild birds serve as their major reservoirs. Epidemiological surveys of bird populations at various geographical scales can clarify key aspects of the eco-epidemiology of these viruses. In this study, we aimed at exploring the presence of flaviviruses in the western Mediterranean by sampling breeding populations of the yellow-legged gull (Larus michahellis, a widely distributed, anthropophilic, and abundant seabird species. For 3 years, we sampled eggs from 19 breeding colonies in Spain, France, Algeria, and Tunisia. First, ELISAs were used to determine if the eggs contained antibodies against flaviviruses. Second, neutralization assays were used to identify the specific flaviviruses present. Finally, for colonies in which ELISA-positive eggs had been found, chick serum samples and potential vectors, culicid mosquitoes and soft ticks (Ornithodoros maritimus, were collected and analyzed using serology and PCR, respectively. The prevalence of flavivirus-specific antibodies in eggs was highly spatially heterogeneous. In northeastern Spain, on the Medes Islands and in the nearby village of L'Escala, 56% of eggs had antibodies against the flavivirus envelope protein, but were negative for neutralizing antibodies against three common flaviviruses: West Nile, Usutu, and tick-borne encephalitis viruses. Furthermore, little evidence of past flavivirus exposure was obtained for the other colonies. A subset of the Ornithodoros ticks from Medes screened for flaviviral RNA tested positive for a virus whose NS5 gene was 95% similar to that of Meaban virus, a flavivirus previously isolated from ticks of Larus argentatus in western France. All ELISA-positive samples subsequently tested positive for Meaban virus neutralizing antibodies. This study shows that gulls in the western Mediterranean Basin are exposed to a tick-borne Meaban-like virus, which underscores the need of exploring

  19. Amplification of 'variola virus-specific' sequences in German cowpox virus isolates.

    Science.gov (United States)

    Meyer, H; Neubauer, H; Pfeffer, M

    2002-02-01

    In 1995 a polymerase chain reaction (PCR) protocol describing the specific detection of variola virus, the causative agent of smallpox, was published by Knight and others. Virulent variola major strains could be differentiated from less virulent variola minor strains because of the distinct amplicon sizes. Here, we applied this PCR protocol to DNA from various orthopoxvirus isolates. There was no amplification with the orthopoxvirus species vaccinia, monkeypox, mousepox, or camelpox viruses. However, amplification was observed in six out of 15 cowpox virus strains investigated. The size of the amplicons corresponded exactly with the size described for variola minor strains and the nucleotide sequence identity accounted for 97%. Findings are discussed with respect to the evolution of orthopoxvirus species assuming that variola virus most probably stems from a rodent-transmitted cowpox virus-like progenitor. PMID:11911586

  20. Influenza A Viruses from Overwintering and Spring-Migrating Waterfowl in the Lake Erie Basin, United States.

    Science.gov (United States)

    Nolting, Jacqueline M; Fries, Anthony C; Gates, Robert J; Bowman, Andrew S; Slemons, Richard D

    2016-05-01

    Influenza A virus (IAV) surveillance in migratory waterfowl in the United States has primarily occurred during late summer and the autumn southern migration. Data concerning the presence and ecology of IAVs in waterfowl during winter and spring seasons in the U.S. northern latitudes have been limited, mainly due to limited access to waterfowl for sampling. The southwestern Lake Erie Basin is an important stopover site for waterfowl during migration periods, and over the past 28 years, 8.72% of waterfowl sampled in this geographic location have been positive for IAV recovery during summer and autumn (June-December). To gain a better understanding of influenza A viral dynamics in waterfowl populations during winter and spring migration (February through April), cloacal swabs were collected from overwintering and spring-migrating waterfowl in Ohio and Michigan in 2006, 2007, 2013, and 2014. A total of 740 cloacal swabs were collected and tested using virus isolation in embryonating chicken eggs, resulting in the recovery of 33 (4.5%) IAV isolates. The influenza A isolates were recovered from eight waterfowl species in the order Anseriformes. Antigenically, the IAV isolates represent 15 distinct hemagglutinin (HA) and neuraminidase (NA) combinations, with seven (21%) of the isolates reported as mixed infections based on antigenic HA subtyping, NA subtyping, or both. This effort demonstrates the presence of antigenically diverse IAV in waterfowl during overwintering and spring migration at northern latitudes in the United States, thereby contributing to the understanding of the maintenance of diversity among waterfowl-origin IAVs. PMID:27309062

  1. Gene : CBRC-PABE-05-0024 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available 69 [Monkeypox virus] 2e-25 44% MLYILYIVYTCYIFYISYIHMLYILYVVYTYVIYSICRIYICYIFYMSYIHMLYILYVVYTYVIYSICRIYICYIFY...MSYIHMLYILYVVYTYVIYLYVVYMLYILYVVYTCYIFYMSYIHVIYSICRIYMLYILYVVYTCYIFYMSYIHVIYSICRIYICYISICRIYICYISICRIYICYISICRIYICYISIC

  2. The complete nucleotide sequence and genome organization of Fig cryptic virus, a novel bipartite dsRNA virus infecting fig, widely distributed in the Mediterranean basin.

    Science.gov (United States)

    Elbeaino, Toufic; Kubaa, Raied Abou; Digiaro, Michele; Minafra, Angelantonio; Martelli, Giovanni P

    2011-06-01

    Two double-stranded RNA (dsRNA) segments of a virus with a bipartite genome identified in fig (Ficus carica L.) and denoted Fig cryptic virus (FCV) were cloned and sequenced. Viral dsRNAs are 1696 bp (RNA-1) and 1415 bp (RNA-2) in size. RNA-1 contains a single ORF (1419 nt) potentially encoding a 54 kDa protein and comprising the conserved amino acid motifs of the RNA-dependent RNA polymerase (RdRp) domain of species of the genus Alphacryptovirus. Its full-length amino acid sequence has the highest identity with Raphanus sativus cryptic virus 2 (RsCV-2) (36%), Beet cryptic virus 3 (BCV-3) (36%) and Fragaria chiloensis cryptic virus (FCCV) (34%). RNA-2 has also a single ORF (1014 nt) coding for a polypeptide with a predicted molecular mass of 38 kDa, identified as the viral coat protein (CP). In a phylogenetic tree constructed with the amino acid sequences of the RdRp domain, FCV clusters in a clade comprising BCV-3 and a number of tentative species of the genus Alphacryptovirus. FCV is not mechanically transmissible. It was detected in fig orchards of six Mediterranean countries (Albania, Algeria, Italy, Lebanon, Syria and Tunisia) where it does not seem to induce a visible disease.

  3. Co-administration of the broad-spectrum antiviral, brincidofovir (CMX001), with smallpox vaccine does not compromise vaccine protection in mice challenged with ectromelia virus.

    Science.gov (United States)

    Parker, Scott; Crump, Ryan; Foster, Scott; Hartzler, Hollyce; Hembrador, Ed; Lanier, E Randall; Painter, George; Schriewer, Jill; Trost, Lawrence C; Buller, R Mark

    2014-11-01

    Natural orthopoxvirus outbreaks such as vaccinia, cowpox, cattlepox and buffalopox continue to cause morbidity in the human population. Monkeypox virus remains a significant agent of morbidity and mortality in Africa. Furthermore, monkeypox virus's broad host-range and expanding environs make it of particular concern as an emerging human pathogen. Monkeypox virus and variola virus (the etiological agent of smallpox) are both potential agents of bioterrorism. The first line response to orthopoxvirus disease is through vaccination with first-generation and second-generation vaccines, such as Dryvax and ACAM2000. Although these vaccines provide excellent protection, their widespread use is impeded by the high level of adverse events associated with vaccination using live, attenuated virus. It is possible that vaccines could be used in combination with antiviral drugs to reduce the incidence and severity of vaccine-associated adverse events, or as a preventive in individuals with uncertain exposure status or contraindication to vaccination. We have used the intranasal mousepox (ectromelia) model to evaluate the efficacy of vaccination with Dryvax or ACAM2000 in conjunction with treatment using the broad spectrum antiviral, brincidofovir (BCV, CMX001). We found that co-treatment with BCV reduced the severity of vaccination-associated lesion development. Although the immune response to vaccination was quantifiably attenuated, vaccination combined with BCV treatment did not alter the development of full protective immunity, even when administered two days following ectromelia challenge. Studies with a non-replicating vaccine, ACAM3000 (MVA), confirmed that BCV's mechanism of attenuating the immune response following vaccination with live virus was, as expected, by limiting viral replication and not through inhibition of the immune system. These studies suggest that, in the setting of post-exposure prophylaxis, co-administration of BCV with vaccination should be considered

  4. SEROLOGICAL DETECTION OF HEPATITIS A VIRUS IN FREE-RANGING NEOTROPICAL PRIMATES (Sapajus spp., Alouatta caraya) FROM THE PARANÁ RIVER BASIN, BRAZIL.

    Science.gov (United States)

    Svoboda, Walfrido Kühl; Soares, Manoel do Carmo Pereira; Alves, Max Moreira; Rocha, Tatiana Carneiro; Gomes, Eliane Carneiro; Menoncin, Fabiana; Batista, Paulo Mira; Silva, Lineu Roberto da; Headley, Selwyn Arlington; Hilst, Carmen Lúcia Scortecci; Aguiar, Lucas M; Ludwig, Gabriela; Passos, Fernando de Camargo; Souza Jr, Júlio Cesar de; Navarro, Italmar Teodorico

    2016-01-01

    Nonhuman primates are considered as the natural hosts of Hepatitis A virus (HAV), as well as other pathogens, and can serve as natural sentinels to investigate epizootics and endemic diseases that are of public health importance. During this study, blood samples were collected from 112 Neotropical primates (NTPs) (Sapajus nigritus and S. cay, n = 75; Alouatta caraya, n = 37) trap-captured at the Paraná River basin, Brazil, located between the States of Paraná and Mato Grosso do Sul. Anti-HAV IgG antibodies were detected in 4.5% (5/112) of NTPs, specifically in 6.7% (5/75) of Sapajus spp. and 0% (0/37) of A. caraya. In addition, all samples were negative for the presence of IgM anti-HAV antibodies. These results suggest that free-ranging NTPs were exposed to HAV within the geographical regions evaluated.

  5. SEROLOGICAL DETECTION OF HEPATITIS A VIRUS IN FREE-RANGING NEOTROPICAL PRIMATES (Sapajus spp., Alouatta caraya FROM THE PARANÁ RIVER BASIN, BRAZIL

    Directory of Open Access Journals (Sweden)

    Walfrido Kühl SVOBODA

    2016-01-01

    Full Text Available Nonhuman primates are considered as the natural hosts of Hepatitis A virus (HAV, as well as other pathogens, and can serve as natural sentinels to investigate epizootics and endemic diseases that are of public health importance. During this study, blood samples were collected from 112 Neotropical primates (NTPs (Sapajus nigritus and S. cay, n = 75; Alouatta caraya, n = 37 trap-captured at the Paraná River basin, Brazil, located between the States of Paraná and Mato Grosso do Sul. Anti-HAV IgG antibodies were detected in 4.5% (5/112 of NTPs, specifically in 6.7% (5/75 of Sapajus spp. and 0% (0/37 of A. caraya. In addition, all samples were negative for the presence of IgM anti-HAV antibodies. These results suggest that free-ranging NTPs were exposed to HAV within the geographical regions evaluated.

  6. Serological evidence for Saint Louis encephalitis virus in free-ranging New World monkeys and horses within the upper Paraná River basin region, Southern Brazil

    Directory of Open Access Journals (Sweden)

    Walfrido Kühl Svoboda

    2014-06-01

    Full Text Available Introduction Saint Louis encephalitis virus (SLEV primarily occurs in the Americas and produces disease predominantly in humans. This study investigated the serological presence of SLEV in nonhuman primates and horses from southern Brazil. Methods From June 2004 to December 2005, sera from 133 monkeys (Alouatta caraya, n=43; Sapajus nigritus, n=64; Sapajus cay, n=26 trap-captured at the Paraná River basin region and 23 blood samples from farm horses were obtained and used for the serological detection of a panel of 19 arboviruses. All samples were analyzed in a hemagglutination inhibition (HI assay; positive monkey samples were confirmed in a mouse neutralization test (MNT. Additionally, all blood samples were inoculated into C6/36 cell culture for viral isolation. Results Positive seroreactivity was only observed for SLEV. A prevalence of SLEV antibodies in sera was detected in Alouatta caraya (11.6%; 5/43, Sapajus nigritus (12.5%; 8/64, and S. cay (30.8%; 8/26 monkeys with the HI assay. Of the monkeys, 2.3% (1/42 of A. caraya, 6.3% 94/64 of S. nigritus, and 15.4% (4/26 of S. cay were positive for SLEV in the MNT. Additionally, SLEV antibodies were detected by HI in 39.1% (9/23 of the horses evaluated in this study. Arboviruses were not isolated from any blood sample. Conclusions These results confirmed the presence of SLEV in nonhuman primates and horses from southern Brazil. These findings most likely represent the first detection of this virus in nonhuman primates beyond the Amazon region. The detection of SLEV in animals within a geographical region distant from the Amazon basin suggests that there may be widespread and undiagnosed dissemination of this disease in Brazil.

  7. Complete Genome Sequence of Mayaro Virus Imported from the Amazon Basin to São Paulo State, Brazil.

    Science.gov (United States)

    Mota, Mânlio Tasso Oliveira; Vedovello, Danila; Estofolete, Cassia; Malossi, Camila Dantas; Araújo, João Pessoa; Nogueira, Maurício Lacerda

    2015-11-25

    Mayaro (MAYV) is a neglected arbovirus from the tropical Americas. Here, we report the complete genome of an MAYV isolate from a patient returning from the Amazon basin and complaining of arthralgia, high fever, and headache, who was attended at an emergency service of São José do Rio Preto, São Paulo state, Brazil.

  8. Ectromelia Virus Infections of Mice as a Model to Support the Licensure of Anti-Orthopoxvirus Therapeutics

    Directory of Open Access Journals (Sweden)

    R. Mark Buller

    2010-09-01

    Full Text Available The absence of herd immunity to orthopoxviruses and the concern that variola or monkeypox viruses could be used for bioterroristic activities has stimulated the development of therapeutics and safer prophylactics. One major limitation in this process is the lack of accessible human orthopoxvirus infections for clinical efficacy trials; however, drug licensure can be based on orthopoxvirus animal challenge models as described in the “Animal Efficacy Rule”. One such challenge model uses ectromelia virus, an orthopoxvirus, whose natural host is the mouse and is the etiological agent of mousepox. The genetic similarity of ectromelia virus to variola and monkeypox viruses, the common features of the resulting disease, and the convenience of the mouse as a laboratory animal underscores its utility in the study of orthopoxvirus pathogenesis and in the development of therapeutics and prophylactics. In this review we outline how mousepox has been used as a model for smallpox. We also discuss mousepox in the context of mouse strain, route of infection, infectious dose, disease progression, and recovery from infection.

  9. Efficacy of CMX001 as a Prophylactic and Presymptomatic Antiviral Agent in New Zealand White Rabbits Infected with Rabbitpox Virus, a Model for Orthopoxvirus Infections of Humans

    Directory of Open Access Journals (Sweden)

    Richard W. Moyer

    2011-01-01

    Full Text Available CMX001, a lipophilic nucleotide analog formed by covalently linking 3‑(hexdecyloxypropan-1-ol to cidofovir (CDV, is being developed as a treatment for smallpox. CMX001 has dramatically increased potency versus CDV against all dsDNA viruses and, in contrast to CDV, is orally available and has shown no evidence of nephrotoxicity in healthy volunteers or severely ill transplant patients to date. Although smallpox has been eliminated from the environment, treatments are urgently being sought due to the risk of smallpox being used as a bioterrorism agent and for monkeypox virus, a zoonotic disease of Africa, and adverse reactions to smallpox virus vaccinations. In the absence of human cases of smallpox, new treatments must be tested for efficacy in animal models. Here we first review and discuss the rabbitpox virus (RPV infection of New Zealand White rabbits as a model for smallpox to test the efficacy of CMX001 as a prophylactic and early disease antiviral. Our results should also be applicable to monkeypox virus infections and for treatment of adverse reactions to smallpox vaccination.

  10. Chasing Jenner's vaccine: revisiting cowpox virus classification.

    Directory of Open Access Journals (Sweden)

    Darin S Carroll

    Full Text Available Cowpox virus (CPXV is described as the source of the first vaccine used to prevent the onset and spread of an infectious disease. It is one of the earliest described members of the genus Orthopoxvirus, which includes the viruses that cause smallpox and monkeypox in humans. Both the historic and current literature describe "cowpox" as a disease with a single etiologic agent. Genotypic data presented herein indicate that CPXV is not a single species, but a composite of several (up to 5 species that can infect cows, humans, and other animals. The practice of naming agents after the host in which the resultant disease manifests obfuscates the true taxonomic relationships of "cowpox" isolates. These data support the elevation of as many as four new species within the traditional "cowpox" group and suggest that both wild and modern vaccine strains of Vaccinia virus are most closely related to CPXV of continental Europe rather than the United Kingdom, the homeland of the vaccine.

  11. A Multiplex PCR/LDR Assay for the Simultaneous Identification of Category A Infectious Pathogens: Agents of Viral Hemorrhagic Fever and Variola Virus.

    Directory of Open Access Journals (Sweden)

    Sanchita Das

    Full Text Available CDC designated category A infectious agents pose a major risk to national security and require special action for public health preparedness. They include viruses that cause viral hemorrhagic fever (VHF syndrome as well as variola virus, the agent of smallpox. VHF is characterized by hemorrhage and fever with multi-organ failure leading to high morbidity and mortality. Smallpox, a prior scourge, has been eradicated for decades, making it a particularly serious threat if released nefariously in the essentially non-immune world population. Early detection of the causative agents, and the ability to distinguish them from other pathogens, is essential to contain outbreaks, implement proper control measures, and prevent morbidity and mortality. We have developed a multiplex detection assay that uses several species-specific PCR primers to generate amplicons from multiple pathogens; these are then targeted in a ligase detection reaction (LDR. The resultant fluorescently-labeled ligation products are detected on a universal array enabling simultaneous identification of the pathogens. The assay was evaluated on 32 different isolates associated with VHF (ebolavirus, marburgvirus, Crimean Congo hemorrhagic fever virus, Lassa fever virus, Rift Valley fever virus, Dengue virus, and Yellow fever virus as well as variola virus and vaccinia virus (the agent of smallpox and its vaccine strain, respectively. The assay was able to detect all viruses tested, including 8 sequences representative of different variola virus strains from the CDC repository. It does not cross react with other emerging zoonoses such as monkeypox virus or cowpox virus, or six flaviviruses tested (St. Louis encephalitis virus, Murray Valley encephalitis virus, Powassan virus, Tick-borne encephalitis virus, West Nile virus and Japanese encephalitis virus.

  12. Evidence for Persistence of Ectromelia Virus in Inbred Mice, Recrudescence Following Immunosuppression and Transmission to Naïve Mice

    Science.gov (United States)

    Sakala, Isaac G.; Chaudhri, Geeta; Scalzo, Anthony A.; Eldi, Preethi; Newsome, Timothy P.; Buller, Robert M.; Karupiah, Gunasegaran

    2015-01-01

    Orthopoxviruses (OPV), including variola, vaccinia, monkeypox, cowpox and ectromelia viruses cause acute infections in their hosts. With the exception of variola virus (VARV), the etiological agent of smallpox, other OPV have been reported to persist in a variety of animal species following natural or experimental infection. Despite the implications and significance for the ecology and epidemiology of diseases these viruses cause, those reports have never been thoroughly investigated. We used the mouse pathogen ectromelia virus (ECTV), the agent of mousepox and a close relative of VARV to investigate virus persistence in inbred mice. We provide evidence that ECTV causes a persistent infection in some susceptible strains of mice in which low levels of virus genomes were detected in various tissues late in infection. The bone marrow (BM) and blood appeared to be key sites of persistence. Contemporaneous with virus persistence, antiviral CD8 T cell responses were demonstrable over the entire 25-week study period, with a change in the immunodominance hierarchy evident during the first 3 weeks. Some virus-encoded host response modifiers were found to modulate virus persistence whereas host genes encoded by the NKC and MHC class I reduced the potential for persistence. When susceptible strains of mice that had apparently recovered from infection were subjected to sustained immunosuppression with cyclophosphamide (CTX), animals succumbed to mousepox with high titers of infectious virus in various organs. CTX treated index mice transmitted virus to, and caused disease in, co-housed naïve mice. The most surprising but significant finding was that immunosuppression of disease-resistant C57BL/6 mice several weeks after recovery from primary infection generated high titers of virus in multiple tissues. Resistant mice showed no evidence of a persistent infection. This is the strongest evidence that ECTV can persist in inbred mice, regardless of their resistance status. PMID

  13. A novel highly reproducible and lethal nonhuman primate model for orthopox virus infection.

    Directory of Open Access Journals (Sweden)

    Marit Kramski

    Full Text Available The intentional re-introduction of Variola virus (VARV, the agent of smallpox, into the human population is of great concern due its bio-terroristic potential. Moreover, zoonotic infections with Cowpox (CPXV and Monkeypox virus (MPXV cause severe diseases in humans. Smallpox vaccines presently available can have severe adverse effects that are no longer acceptable. The efficacy and safety of new vaccines and antiviral drugs for use in humans can only be demonstrated in animal models. The existing nonhuman primate models, using VARV and MPXV, need very high viral doses that have to be applied intravenously or intratracheally to induce a lethal infection in macaques. To overcome these drawbacks, the infectivity and pathogenicity of a particular CPXV was evaluated in the common marmoset (Callithrix jacchus.A CPXV named calpox virus was isolated from a lethal orthopox virus (OPV outbreak in New World monkeys. We demonstrated that marmosets infected with calpox virus, not only via the intravenous but also the intranasal route, reproducibly develop symptoms resembling smallpox in humans. Infected animals died within 1-3 days after onset of symptoms, even when very low infectious viral doses of 5x10(2 pfu were applied intranasally. Infectious virus was demonstrated in blood, saliva and all organs analyzed.We present the first characterization of a new OPV infection model inducing a disease in common marmosets comparable to smallpox in humans. Intranasal virus inoculation mimicking the natural route of smallpox infection led to reproducible infection. In vivo titration resulted in an MID(50 (minimal monkey infectious dose 50% of 8.3x10(2 pfu of calpox virus which is approximately 10,000-fold lower than MPXV and VARV doses applied in the macaque models. Therefore, the calpox virus/marmoset model is a suitable nonhuman primate model for the validation of vaccines and antiviral drugs. Furthermore, this model can help study mechanisms of OPV pathogenesis.

  14. Heartland Virus

    Science.gov (United States)

    ... Vector-Borne Diseases (DVBD) NCEZID Share Compartir Heartland virus On this Page What is Heartland virus? How ... Do I Need to Know? What is Heartland virus? Heartland virus belongs to a family of viruses ...

  15. Field detection of eastern equine encephalitis virus in the Amazon Basin region of Peru using reverse transcription-polymerase chain reaction adapted for field identification of arthropod-borne pathogens.

    Science.gov (United States)

    O'Guinn, Monica L; Lee, John S; Kondig, John P; Fernandez, Roberto; Carbajal, Faustino

    2004-02-01

    In support of efforts to develop rapid diagnostic assays for use in the field, reverse transcription-polymerase chain reaction (RT-PCR) assays were developed to detect arboviruses circulating in the Amazon Basin region of Peru. Previous knowledge of arthropod/pathogen relationships allowed a focused evaluation to be conducted in November 2000 that assessed the feasibility and reliability of a mobile, rapid, field-expedient RT-PCR diagnostic system aimed at detecting eastern equine encephalitis virus (EEEV) in Culex (Melanoconion) pedroi mosquitoes. Modifications were made to a commercially available mobile molecular laboratory kit and assay procedures were tailored for use under harsh environmental conditions with field-collected and field-processed mosquitoes. From CO2 baited mosquito light traps, 3,227 Cx. (Mel.) pedroi mosquitoes were collected and sorted into 117 pools. The pools were processed and assayed in the field by RT-PCR and five of those pools were found positive for EEEV. Laboratory sequence analysis confirmed the presence of two distinct subtypes of EEEV.

  16. Andes hantavirus variant in rodents, southern Amazon Basin, Peru.

    Science.gov (United States)

    Razuri, Hugo; Tokarz, Rafal; Ghersi, Bruno M; Salmon-Mulanovich, Gabriela; Guezala, M Claudia; Albujar, Christian; Mendoza, A Patricia; Tinoco, Yeny O; Cruz, Christopher; Silva, Maria; Vasquez, Alicia; Pacheco, Víctor; Ströher, Ute; Guerrero, Lisa Wiggleton; Cannon, Deborah; Nichol, Stuart T; Hirschberg, David L; Lipkin, W Ian; Bausch, Daniel G; Montgomery, Joel M

    2014-02-01

    We investigated hantaviruses in rodents in the southern Amazon Basin of Peru and identified an Andes virus variant from Neacomys spinosus mice. This finding extends the known range of this virus in South America and the range of recognized hantaviruses in Peru. Further studies of the epizoology of hantaviruses in this region are warranted.

  17. Use of Vaccinia Virus Smallpox Vaccine in Laboratory and Health Care Personnel at Risk for Occupational Exposure to Orthopoxviruses - Recommendations of the Advisory Committee on Immunization Practices (ACIP), 2015.

    Science.gov (United States)

    Petersen, Brett W; Harms, Tiara J; Reynolds, Mary G; Harrison, Lee H

    2016-03-18

    On June 25, 2015, the Advisory Committee on Immunization Practices (ACIP) recommended routine vaccination with live smallpox (vaccinia) vaccine (ACAM2000) for laboratory personnel who directly handle 1) cultures or 2) animals contaminated or infected with replication-competent vaccinia virus, recombinant vaccinia viruses derived from replication-competent vaccinia strains (i.e., those that are capable of causing clinical infection and producing infectious virus in humans), or other orthopoxviruses that infect humans (e.g., monkeypox, cowpox, and variola) (recommendation category: A, evidence type 2 [Box]). Health care personnel (e.g., physicians and nurses) who currently treat or anticipate treating patients with vaccinia virus infections and whose contact with replication-competent vaccinia viruses is limited to contaminated materials (e.g., dressings) and persons administering ACAM2000 smallpox vaccine who adhere to appropriate infection prevention measures can be offered vaccination with ACAM2000 (recommendation category: B, evidence type 2 [Box]). These revised recommendations update the previous ACIP recommendations for nonemergency use of vaccinia virus smallpox vaccine for laboratory and health care personnel at risk for occupational exposure to orthopoxviruses (1). Since 2001, when the previous ACIP recommendations were developed, ACAM2000 has replaced Dryvax as the only smallpox vaccine licensed by the U.S. Food and Drug Administration (FDA) and available for use in the United States (2). These recommendations contain information on ACAM2000 and its use in laboratory and health care personnel at risk for occupational exposure to orthopoxviruses.

  18. ECHO virus

    Science.gov (United States)

    ... page: //medlineplus.gov/ency/article/001340.htm ECHO virus To use the sharing features on this page, please enable JavaScript. Enteric cytopathic human orphan (ECHO) viruses are a group of viruses that lead to ...

  19. Zika Virus

    Science.gov (United States)

    Zika is a virus that is spread mostly by mosquitoes. A pregnant mother can pass it to ... through blood transfusions. There have been outbreaks of Zika virus in the United States, Africa, Southeast Asia, ...

  20. Chikungunya virus

    Science.gov (United States)

    Chikungunya virus infection; Chikungunya ... Where Chikungunya is found Before 2013, the virus was found in Africa, Asia, Europe, and the Indian and Pacific oceans. In late 2013, outbreaks occurred for the first time in the ...

  1. Chikungunya Virus

    Science.gov (United States)

    ... Gaines, PhD, MPH, MA, CHES Differentiating Chikungunya From Dengue: A Clinical Challenge For Travelers CDC Travelers' Health Chikungunya Virus Home Prevention Transmission Symptoms & Treatment Geographic Distribution Chikungunya virus in ...

  2. Epidemiology of viruses causing chronic hepatitis among populations from the Amazon Basin and related ecosystems Epidemiología de las hepatitis crónicas con carácter vírico en las comunidades indígenas de la cuenca amazónica y de otros ecosistemas similares

    Directory of Open Access Journals (Sweden)

    José M. Echevarría

    2003-12-01

    Full Text Available On the last twenty years, viral hepatitis has emerged as a serious problem in almost all the Amerindian communities studied in the Amazon Basin and in other Amazon-related ecological systems from the North and Center of South America. Studies performed on communities from Bolivia, Brazil, Colombia, Peru and Venezuela have shown a high endemicity of the hepatitis B virus (HBV infection all over the region, which is frequently associated to a high prevalence of infection by hepatitis D virus among the chronic HBV carriers. Circulation of both agents responds mainly to horizontal virus transmission during childhood through mechanisms that are not fully understood. By contrast, infection by hepatitis C virus (HCV, which is present in all the urban areas of South America, is still very uncommon among them. At the moment, there is not data enough to evaluate properly the true incidence that such endemicity may have on the health of the populations affected. Since viral transmission might be operated by mechanisms that could not be acting in other areas of the World, it seems essential to investigate such mechanisms and to prevent the introduction of HCV into these populations, which consequences for health could be very serious.A lo largo de los últimos veinte años, las hepatitis víricas se han revelado como un importante problema para las comunidades indígenas de la cuenca amazónica y de otros ecosistemas similares del norte y centro de Sudamérica. Los estudios realizados en comunidades de Bolivia, Brasil, Colombia, Perú y Venezuela han demostrado una alta propensión endémica para la infección por el virus de la hepatitis B, que se asocia con frecuencia a una elevada prevalencia de coinfección con el virus de la hepatitis D entre los portadores crónicos. La circulación de ambos agentes responde a su transmisión horizontal durante la infancia, a través de mecanismos aún poco conocidos. Por el contrario, la infección por el virus de la

  3. Computer Virus

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    Computer viruses are small software programs that are designed to spread from one computerto another and to interfere with computer operation.A virus might delete data on your computer,use your e-mail program to spread itself to othercomputers,or even erase everything on your hard disk.Viruses are most easily spread by attach-ments in e-mail messages or instant messaging messages.That is why it is essential that you never

  4. Viruses of cucurbit crops in the Mediterranean region: an ever-changing picture.

    Science.gov (United States)

    Lecoq, Hervé; Desbiez, Cécile

    2012-01-01

    Cucurbit crops may be affected by at least 28 different viruses in the Mediterranean basin. Some of these viruses are widely distributed and cause severe yield losses while others are restricted to limited areas or specific crops, and have only a negligible economic impact. A striking feature of cucurbit viruses in the Mediterranean basin is their always increasing diversity. Indeed, new viruses are regularly isolated and over the past 35 years one "new" cucurbit virus has been reported on average every 2 years. Among these "new" viruses some were already reported in other parts of the world, but others such as Zucchini yellow mosaic virus (ZYMV), one of the most severe cucurbit viruses and Cucurbit aphid-borne yellows virus (CABYV), one of the most prevalent cucurbit viruses, were first described in the Mediterranean area. Why this region may be a potential "hot-spot" for cucurbit virus diversity is not fully known. This could be related to the diversity of cropping practices, of cultivar types but also to the important commercial exchanges that always prevailed in this part of the world. This chapter describes the major cucurbit viruses occurring in the Mediterranean basin, discusses factors involved in their emergence and presents options for developing sustainable control strategies. PMID:22682166

  5. Computer Virus

    Institute of Scientific and Technical Information of China (English)

    高振桥

    2002-01-01

    If you work with a computer,it is certain that you can not avoid dealing, with at least one computer virus.But how much do you know about it? Well,actually,a computer virus is not a biological' one as causes illnesses to people.It is a kind of computer program

  6. Fate of human viruses in groundwater recharge systems

    Energy Technology Data Exchange (ETDEWEB)

    Vaughn, J.M.; Landry, E.F.

    1980-03-01

    The overall objective of this research program was to determine the ability of a well-managed tertiary effluent-recharge system to return virologically acceptable water to the groundwater aquifer. The study assessed the quality of waters renovated by indigenous recharge operations and investigated a number of virus-soil interrelationships. The elucidation of the interactions led to the establishment of basin operating criteria for optimizing virus removal. Raw influents, chlorinated tertiary effluents, and renovated wastewater from the aquifer directly beneath a uniquely designed recharge test basin were assayed on a weekly basis for the presence of human enteroviruses and coliform bacteria. High concentrations of viruses were routinely isolated from influents but were isolated only on four occasions from tertiary-treated sewage effluents. In spite of the high quality effluent being recharged, viruses were isolated from the groundwater observation well, indicating their ability to penetrate the unsaturated zone. Results of poliovirus seeding experiments carried out in the test basin clearly indicated the need to operate recharge basins at low (e.g. 1 cm/h) infiltration rates in areas having soil types similar to those found at the study site. The method selected for reducing the test basin infiltration rate involved clogging the basin surface with settled organic material from highly turbid effluent. Alternative methods for slowing infiltration rates are discussed in the text.

  7. Computer viruses

    Science.gov (United States)

    Denning, Peter J.

    1988-01-01

    The worm, Trojan horse, bacterium, and virus are destructive programs that attack information stored in a computer's memory. Virus programs, which propagate by incorporating copies of themselves into other programs, are a growing menace in the late-1980s world of unprotected, networked workstations and personal computers. Limited immunity is offered by memory protection hardware, digitally authenticated object programs,and antibody programs that kill specific viruses. Additional immunity can be gained from the practice of digital hygiene, primarily the refusal to use software from untrusted sources. Full immunity requires attention in a social dimension, the accountability of programmers.

  8. Zika Virus.

    Science.gov (United States)

    Phillips, Jennan A; Neyland, Anavernyel

    2016-08-01

    Zika virus (ZIKV) infections are the latest global public health emergency. Occupational health nurses can protect society by educating workers, women of childbearing age, and others traveling in ZIKV-infected areas about prevention strategies. PMID:27411846

  9. Ebola Virus

    OpenAIRE

    Anusha Rangare Lakshman; Vikas Goya; Hosmar Ganesh Shenoy

    2015-01-01

    The disease Ebola takes its name from the Ebola River situated near a village in the Democratic Republic of Congo, where the disease first appeared in 1976. It is caused by a virus from the Filoviridae family (filovirus). The present outbreak of Ebola Virus Disease (EVD) concerns four countries in West Africa, namely Guinea, Liberia, Sierra Leone and Nigeria till date. Further to widespread transmission of the disease, it has been declared as a Public Health Emergency of International Concern...

  10. Live Virus Smallpox Vaccine

    Science.gov (United States)

    ... A - Z Index SMALLPOX FACT SHEET The Live Virus Smallpox Vaccine The vaccinia virus is the "live ... it cannot cause smallpox. What is a "live virus" vaccine? A "live virus" vaccine is a vaccine ...

  11. Simple technique for in field samples collection in the cases of skin rash illness and subsequent PCR detection of orthopoxviruses and varicella zoster virus.

    Directory of Open Access Journals (Sweden)

    Catherine Dumont

    Full Text Available BACKGROUND: In case of outbreak of rash illness in remote areas, clinically discriminating monkeypox (MPX from severe form of chickenpox and from smallpox remains a concern for first responders. OBJECTIVE: The goal of the study was therefore to use MPX and chickenpox outbreaks in Democratic Republic of Congo (DRC as a test case for establishing a rapid and specific diagnosis in affected remote areas. METHODS: In 2008 and 2009, successive outbreaks of presumed MPX skin rash were reported in Bena Tshiadi, Yangala and Ndesha healthcare districts of the West Kasai province (DRC. Specimens consisting of liquid vesicle dried on filter papers or crusted scabs from healing patients were sampled by first responders. A field analytical facility was deployed nearby in order to carry out a real-time PCR (qPCR assay using genus consensus primers, consensus orthopoxvirus (OPV and smallpox-specific probes spanning over the 14 kD fusion protein encoding gene. A PCR-restriction fragment length polymorphism was used on-site as backup method to confirm the presence of monkeypox virus (MPXV in samples. To complete the differential diagnosis of skin rash, chickenpox was tested in parallel using a commercial qPCR assay. In a post-deployment step, a MPXV-specific pyrosequencing was carried out on all biotinylated amplicons generated on-site in order to confirm the on-site results. RESULTS: Whereas MPXV proved to be the agent causing the rash illness outbreak in the Bena Tshiadi, VZV was the causative agent of the disease in Yangala and Ndesha districts. In addition, each on-site result was later confirmed by MPXV-specific pyrosequencing analysis without any discrepancy. CONCLUSION: This experience of rapid on-site dual use DNA-based differential diagnosis of rash illnesses demonstrates the potential of combining tests specifically identifying bioterrorism agents and agents causing natural outbreaks. This opens the way to rapid on-site DNA-based identification of a

  12. Basin Hopping Graph

    DEFF Research Database (Denmark)

    Kucharik, Marcel; Hofacker, Ivo; Stadler, Peter;

    2014-01-01

    of the folding free energy landscape, however, can provide the relevant information. Results We introduce the basin hopping graph (BHG) as a novel coarse-grained model of folding landscapes. Each vertex of the BHG is a local minimum, which represents the corresponding basin in the landscape. Its edges connect...

  13. Computer Viruses. Technology Update.

    Science.gov (United States)

    Ponder, Tim, Comp.; Ropog, Marty, Comp.; Keating, Joseph, Comp.

    This document provides general information on computer viruses, how to help protect a computer network from them, measures to take if a computer becomes infected. Highlights include the origins of computer viruses; virus contraction; a description of some common virus types (File Virus, Boot Sector/Partition Table Viruses, Trojan Horses, and…

  14. K Basin safety analysis

    International Nuclear Information System (INIS)

    The purpose of this accident safety analysis is to document in detail, analyses whose results were reported in summary form in the K Basins Safety Analysis Report WHC-SD-SNF-SAR-001. The safety analysis addressed the potential for release of radioactive and non-radioactive hazardous material located in the K Basins and their supporting facilities. The safety analysis covers the hazards associated with normal K Basin fuel storage and handling operations, fuel encapsulation, sludge encapsulation, and canister clean-up and disposal. After a review of the Criticality Safety Evaluation of the K Basin activities, the following postulated events were evaluated: Crane failure and casks dropped into loadout pit; Design basis earthquake; Hypothetical loss of basin water accident analysis; Combustion of uranium fuel following dryout; Crane failure and cask dropped onto floor of transfer area; Spent ion exchange shipment for burial; Hydrogen deflagration in ion exchange modules and filters; Release of Chlorine; Power availability and reliability; and Ashfall

  15. K Basin safety analysis

    Energy Technology Data Exchange (ETDEWEB)

    Porten, D.R.; Crowe, R.D.

    1994-12-16

    The purpose of this accident safety analysis is to document in detail, analyses whose results were reported in summary form in the K Basins Safety Analysis Report WHC-SD-SNF-SAR-001. The safety analysis addressed the potential for release of radioactive and non-radioactive hazardous material located in the K Basins and their supporting facilities. The safety analysis covers the hazards associated with normal K Basin fuel storage and handling operations, fuel encapsulation, sludge encapsulation, and canister clean-up and disposal. After a review of the Criticality Safety Evaluation of the K Basin activities, the following postulated events were evaluated: Crane failure and casks dropped into loadout pit; Design basis earthquake; Hypothetical loss of basin water accident analysis; Combustion of uranium fuel following dryout; Crane failure and cask dropped onto floor of transfer area; Spent ion exchange shipment for burial; Hydrogen deflagration in ion exchange modules and filters; Release of Chlorine; Power availability and reliability; and Ashfall.

  16. Ebola Virus

    Directory of Open Access Journals (Sweden)

    Anusha Rangare Lakshman

    2015-09-01

    Full Text Available The disease Ebola takes its name from the Ebola River situated near a village in the Democratic Republic of Congo, where the disease first appeared in 1976. It is caused by a virus from the Filoviridae family (filovirus. The present outbreak of Ebola Virus Disease (EVD concerns four countries in West Africa, namely Guinea, Liberia, Sierra Leone and Nigeria till date. Further to widespread transmission of the disease, it has been declared as a Public Health Emergency of International Concern by the World Health Organisation on 8 August 2014. As of 4 August 2014, countries have reported 1,711 cases (1,070 confirmed, 436 probable, 205 suspect, including 932 deaths. This review paper enlightens about the awareness of Ebola virus and its preventive measures. [Archives Medical Review Journal 2015; 24(3.000: 296-305

  17. Wada basin boundaries and basin cells

    NARCIS (Netherlands)

    Nusse, H.E.; Yorke, J.A.

    1996-01-01

    In dynamical systems examples are common in which two or more attractors coexist, and in such cases the basin boundary is nonempty. We consider a two-dimensional diffeomorphism F (that is, F is an invertible map and both F and its inverse are differentiable with continuous derivatives), which has at

  18. Reserves in western basins

    Energy Technology Data Exchange (ETDEWEB)

    Caldwell, R.H.; Cotton, B.W. [Scotia Group, Dallas, TX (United States)

    1995-04-01

    The objective of this project is to investigate the reserves potential of tight gas reservoirs in three Rocky Mountain basins: the Greater Green River (GGRB), Uinta and Piceance basins. The basins contain vast gas resources that have been estimated in the thousands of Tcf hosted in low permeability clastic reservoirs. This study documents the productive characteristics of these tight reservoirs, requantifies gas in place resources, and characterizes the reserves potential of each basin. The purpose of this work is to promote understanding of the resource and to encourage its exploitation by private industry. At this point in time, the GGRB work has been completed and a final report published. Work is well underway in the Uinta and Piceance basins which are being handled concurrently, with reports on these basins being scheduled for the middle of this year. Since the GGRB portion of the project has been completed, this presentation win focus upon that basin. A key conclusion of this study was the subdivision of the resource, based upon economic and technological considerations, into groupings that have distinct properties with regard to potential for future producibility, economics and risk profile.

  19. Virus diseases of peas, beans, and faba bean in the Mediterranean region.

    Science.gov (United States)

    Makkouk, Khaled; Pappu, Hanu; Kumari, Safaa G

    2012-01-01

    In the Mediterranean region, pea, bean, and faba bean production is affected by around 17 major viruses. These viruses do not have the same ecology and consequently require a variety of different preventive measures to control them. Some of these viruses have a narrow host range, such as Faba bean necrotic yellows virus (FBNYV), and others, such as Alfalfa mosaic virus (AMV) and Cucumber mosaic virus (CMV), a very wide host range. Such features are important when identifying sources of virus inoculum in a region, and the vectors can transmit viruses from natural reservoirs to the crop plants. Some of these viruses are seed borne and, consequently, can be disseminated long distances through infected seeds. Crop losses caused by these viruses are variable, depending on the sensitivity and susceptibility of the crop to infection. Host resistance genes have been identified for some of these viruses, but in others, such as FBNYV, no resistance genes in faba bean have been identified yet. Significant progress was made in developing precise methods for the identification of these viruses, and new virus problems are being identified every year. This chapter is not intended to be a review for pea, bean, and faba bean viruses, but rather focuses on the major viruses which affect these crops in the Mediterranean basin with focus on the progress made over the past two decades. PMID:22682174

  20. Passatempo Virus, a Vaccinia Virus Strain, Brazil

    OpenAIRE

    Leite, Juliana A.; Drumond, Betânia P.; Trindade, Giliane S; Zélia I P Lobato; da Fonseca, Flávio G.; dos Santos, João R.; Madureira, Marieta C.; Guedes, Maria I.M.C.; Ferreira, Jaqueline M. S.; Bonjardim, Cláudio A.; Ferreira, Paulo C. P.; Kroon, Erna G.

    2005-01-01

    Passatempo virus was isolated during a zoonotic outbreak. Biologic features and molecular characterization of hemagglutinin, thymidine kinase, and vaccinia growth factor genes suggested a vaccinia virus infection, which strengthens the idea of the reemergence and circulation of vaccinia virus in Brazil. Molecular polymorphisms indicated that Passatempo virus is a different isolate.

  1. Tulare Basin protection plan

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — The Tulare Basin Protection Plan has been initiated by The Nature Conservancy to elucidate the problems and opportunities of natural diversity protection....

  2. Mitigation : Closed Basin Project

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — The upcoming meeting on waterfowl mitigation for the Closed Basin Project will have several people talk about possible changes to the waterfowl mitigation program....

  3. Early Mesozoic basin aquifers

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — This data set represents the extent of the Early Mesozoic basin aquifers in the states of Massachusettes, Connecticut, New York, New Jersey, Pennsylvania, Maryland,...

  4. California Air Basins

    Data.gov (United States)

    California Department of Resources — Air ResourcesCalifornia Air Resources BoardThe following datasets are from the California Air Resources Board: * arb_california_airbasins - California Air BasinsThe...

  5. Oropuche virus: A virus present but ignored

    Directory of Open Access Journals (Sweden)

    Salim Mattar V.

    2015-09-01

    Full Text Available Bunyaviruses are RNA viruses that affect animals and plants; they have five genera and four of them affect humans: Orthobunyavirus, Nairovirus, Phlebovirus and Hantavirus. All of them are Arbovirus, except Hantavirus. The Orthobunyaviruses comprise Oropouche, Tahyna, La Crosse virus, California encephalitis virus and Heartland virus recently discovered (1. Except for Heartland virus which is transmitted by ticks of the genus Amblyoma, these Phleboviruses have as vectors mosquitoes, which bite small mammals which are able to be as reservoirs amplifiers.

  6. K Basin Hazard Analysis

    Energy Technology Data Exchange (ETDEWEB)

    PECH, S.H.

    2000-08-23

    This report describes the methodology used in conducting the K Basins Hazard Analysis, which provides the foundation for the K Basins Final Safety Analysis Report. This hazard analysis was performed in accordance with guidance provided by DOE-STD-3009-94, Preparation Guide for U. S. Department of Energy Nonreactor Nuclear Facility Safety Analysis Reports and implements the requirements of DOE Order 5480.23, Nuclear Safety Analysis Report.

  7. The Aquitaine basin

    Energy Technology Data Exchange (ETDEWEB)

    Biteau, J.-J.; Le Marrec, A.; Le Vot, M.; Masset, J.-M.

    2006-07-01

    The Aquitaine Basin is located in the southwest of France, between the Gironde Arch in the north and the Pyrenean Mountain Chain in the south. It is a triangular-shaped domain, extending over 35000km{sup 2}. From north to south, six main geological provinces can be identified: (1) the Medoc Platform located south of the Gironde Arch; (2) the Parentis sub-basin; (3) the Landes Saddle; (4) the North Aquitaine Platform; (5) the foreland of the Pyrenees (also known as the Adour, Arzacq and Comminges sub-basins); and (6) the Pyrenean fold-and-thrust belt. Only the Parentis sub-basin, the foreland of the Pyrenean Chain and a minor part of the fold-and-thrust belt itself are proven hydrocarbon provinces. The Aquitaine Basin, in turn, is subdivided into four sub-basins - the Parentis, Adour-Arzacq, Tarbes and Comminges areas. The lozenge shape of these depocentres is related to the Hercynian tectonic framework of the Palaeozoic basement, reactivated during Early Cretaceous rifting. This rift phase aborted at the end of the Albian (prior to the development of an oceanic crust) in response to the beginning of the subduction of the Iberian plate under the European plate. During the Upper Cretaceous, continued subduction led to the creation of northwards-migrating flexural basins. In the Eocene, a paroxysmal phase of compression was responsible for the uplift of the Pyrenean Mountain Chain and for the thin-skinned deformation of the foreland basin. The resulting structuration is limited to the south by the internal core of the chain and to the north by the leading edge of the fold-and-thrust belt, where the Lacq and Meillon gas fields are located. Four main petroleum provinces have been exploited since the Second World War: (1) the oil-prone Parentis sub-basin and (2) salt ridges surrounding the Arzacq and Tarbes sub-basins; and (3) the gas-prone southern Arzacq sub-basin (including the external Pyrenean fold-and-thrust belt and the proximal foreland sub-basin) and (4

  8. Plant Virus Metagenomics: Advances in Virus Discovery.

    Science.gov (United States)

    Roossinck, Marilyn J; Martin, Darren P; Roumagnac, Philippe

    2015-06-01

    In recent years plant viruses have been detected from many environments, including domestic and wild plants and interfaces between these systems-aquatic sources, feces of various animals, and insects. A variety of methods have been employed to study plant virus biodiversity, including enrichment for virus-like particles or virus-specific RNA or DNA, or the extraction of total nucleic acids, followed by next-generation deep sequencing and bioinformatic analyses. All of the methods have some shortcomings, but taken together these studies reveal our surprising lack of knowledge about plant viruses and point to the need for more comprehensive studies. In addition, many new viruses have been discovered, with most virus infections in wild plants appearing asymptomatic, suggesting that virus disease may be a byproduct of domestication. For plant pathologists these studies are providing useful tools to detect viruses, and perhaps to predict future problems that could threaten cultivated plants.

  9. Virus Ebola Asia

    OpenAIRE

    Suharyono Wuryadi

    2012-01-01

    Virus Marburg dan Ebola diklasifikasikan sebagai virus yang sangat menular dan dimasukkan dalam klasifikasi sebagai virus/pathogen dengan derajat biosafety 4, sehingga untuk menanganinya diperlukan laboratorium khusus tingkat 4.

  10. Zika Virus and Pregnancy

    Science.gov (United States)

    ... Education & Events Advocacy For Patients About ACOG Zika Virus and Pregnancy Home For Patients Zika Virus and ... Education Pamphlets - Spanish Share: PEV002, September 2016 Zika Virus and Pregnancy There are risks to your fetus ...

  11. Computer Viruses: An Overview.

    Science.gov (United States)

    Marmion, Dan

    1990-01-01

    Discusses the early history and current proliferation of computer viruses that occur on Macintosh and DOS personal computers, mentions virus detection programs, and offers suggestions for how libraries can protect themselves and their users from damage by computer viruses. (LRW)

  12. Oncogenic viruses and cancer

    Institute of Scientific and Technical Information of China (English)

    Guangxiang; George; Luo; Jing-hsiung; James; Ou

    2015-01-01

    <正>This special issue of the journal is dedicated to the important topic of oncogenic viruses and cancer.It contains seven review articles covering all known oncogenic viruses except for human T-lymphotropic virus type1(HTLV-1).These review articles are contributed by experts on specific viruses and their associated human cancers.Viruses account for about 20%of total human cancer cases.Although many viruses can cause various tumors in animals,only seven of them

  13. Viruses Infecting Reptiles

    OpenAIRE

    Marschang, Rachel E.

    2011-01-01

    A large number of viruses have been described in many different reptiles. These viruses include arboviruses that primarily infect mammals or birds as well as viruses that are specific for reptiles. Interest in arboviruses infecting reptiles has mainly focused on the role reptiles may play in the epidemiology of these viruses, especially over winter. Interest in reptile specific viruses has concentrated on both their importance for reptile medicine as well as virus taxonomy and evolution. The ...

  14. 76 FR 67736 - Implementation of Section 2695 (42 U.S.C. 300ff-131) of Public Law 111-87: Infectious Diseases...

    Science.gov (United States)

    2011-11-02

    ... fevers (Lassa, Marburg, Ebola, Crimean- Congo, and other viruses yet to be identified) \\18\\ \\18\\ For most... reservoir or direct contact with an infected person (e.g. Ebola virus, Marburg virus) or through arthropod...; Ebola viruses; Francisella tularensis; Lassa fever virus; Marburg virus; Monkeypox virus;...

  15. Susceptibility of various Japanese freshwater fish species to an isolate of viral haemorrhagic septicaemia virus (VHSV) genotype IVb

    DEFF Research Database (Denmark)

    Ito, Takafumi; Olesen, Niels Jørgen

    2013-01-01

    Genotype IVb of viral haemorrhagic septicaemia virus (VHSV) was isolated for the first time in the Great Lakes basin in 2003, where it spread and caused mass mortalities in several wild fish species throughout the basin. In order to prevent further spreading of the disease and to assess risks...

  16. Modifed Great Basin Extent (Buffered)

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — Two different great basin perimeter files were intersected and dissolved using ArcGIS 10.2.2 to create the outer perimeter of the great basin for use modeling...

  17. Reversing the indus basin closure

    OpenAIRE

    2011-01-01

    After independence, a swift and extensive development of Indus river basin has intensified commitment of water resources. During dry period, the indication of over commitment and basin closure are visible. In the beginning 2000s, he river basin water resources were committed to more than 99% without any environmental flows. The paper tries to unfold drivers closing the Indus basin and the scope for change. Defining and implementing water allocation mechanism to ascertain equity, sustainabilit...

  18. Single-basined choice

    NARCIS (Netherlands)

    Bossert, W.; Peters, H.J.M.

    2013-01-01

    Single-basined preferences generalize single-dipped preferences by allowing for multiple worst elements. These preferences have played an important role in areas such as voting, strategy-proofness and matching problems. We examine the notion of single-basinedness in a choice-theoretic setting. In co

  19. The Mediterranean basin

    DEFF Research Database (Denmark)

    Tomas, Carmen; Sanchez Sanchez, Juan Jose; Barbaro, A.;

    2008-01-01

    genetically from the rest of the populations in the Mediterranean area. This result supports the hypothesis of a low incidence of the south-north genetic interchange at the western shores of the Mediterranean basin. A low genetic distance was found between populations in the Middle East and the western part...

  20. Bransfield Basin and Cordilleran Orogenesis

    Science.gov (United States)

    Dalziel, I. W.; Austin, J. A.; Barker, D. H.; Christensen, G. L.

    2003-12-01

    Tectonic uplift of the Andean Cordillera was initiated in the mid-Cretaceous with inversion of a composite marginal basin along 7500 km of the continental margin of South America, from Peru to Tierra del Fuego and the North Scotia Ridge. In the southernmost Andes, from 50-56 degrees S, the quasi-oceanic floor of this basin is preserved in the obducted ophiolitic rocks of the Rocas Verdes (Green Rocks) basin. We suggest that the basin beneath Bransfield Strait, 61-64 degrees S, separating the South Shetland Islands from the Antarctic Peninsula, constitutes a modern analog for the Rocas Verdes basin. Marine geophysical studies of Bransfield basin have been undertaken over the past 12 years by the Institute for Geophysics, University of Texas at Austin, under the auspices of the Ocean Sciences Division and United States Antarctic Program, National Science Foundation. These studies have elucidated the structure and evolution of Bransfield basin for comparison with the Rocas Verdes basin, with a view to eventual forward modeling of the evolution of a hypothetical cordilleran orogen by compression and inversion of the basin. These are the processes that can be observed in the tectonic transformation of the Rocas Verdes basin into the southernmost Andean cordillera, as South America moved rapidly westward in an Atlantic-Indian ocean hot-spot reference frame during the mid-Cretaceous. Multi-channel reflection seismic data from the Bransfield basin reveal an asymmetric structural architecture characterized by steeply-dipping normal faults flanking the South Shetlands island arc and gently dipping listric normal faults along the Antarctic Peninsula margin. Normal fault polarity reversals appear to be related to distributed loci of magmatic activity within the basin. This architecture is remarkably similar to that deduced from field structural studies of the Rocas Verdes basin. Notably, the oceanward-dipping, low angle normal faults along the Antarctic Peninsula margin

  1. Petroleum exploration potential of Tamtsag Basin

    Institute of Scientific and Technical Information of China (English)

    SUN Guo-qing; GUO Qing-xia; ZHANG Ya-jin; ZHAO Hong-wen

    2004-01-01

    The Tamtsag Basin is located in the extreme eastern portion of the Mongolia. The Basin and its counterpart in China (the Hailar Basin) are united a whole basin on the structural setting. In recent years, the Tamtsag Basin attracts more and more attention with the important exploration discovered in the 19th block by SOCO and in Hailar Basin of China. This paper discusses the exploration potential of Tamtsag Basin from the viewpoint of petroleum geology.

  2. Viruses Infecting Reptiles

    Directory of Open Access Journals (Sweden)

    Rachel E. Marschang

    2011-11-01

    Full Text Available A large number of viruses have been described in many different reptiles. These viruses include arboviruses that primarily infect mammals or birds as well as viruses that are specific for reptiles. Interest in arboviruses infecting reptiles has mainly focused on the role reptiles may play in the epidemiology of these viruses, especially over winter. Interest in reptile specific viruses has concentrated on both their importance for reptile medicine as well as virus taxonomy and evolution. The impact of many viral infections on reptile health is not known. Koch’s postulates have only been fulfilled for a limited number of reptilian viruses. As diagnostic testing becomes more sensitive, multiple infections with various viruses and other infectious agents are also being detected. In most cases the interactions between these different agents are not known. This review provides an update on viruses described in reptiles, the animal species in which they have been detected, and what is known about their taxonomic positions.

  3. Canterbury Basin Sea Level

    OpenAIRE

    Fulthorpe, C. S.; Institute for Geophysics John A. and Katherine G. Jackson School of Geosciences The University of Texas at Austin J.J. Pickle Research Campus, Building 196 (ROC) 10100 Burnet Road (R2200) Austin TX 78758-4445 USA; Hoyanagi, K.; Department of Geology Faculty of Science Shinshu University 3-1-1 Asahi, Matsumoto 390-8621 Japan; Blum, P.; United States Implementing Organization Integrated Ocean Drilling Program Texas A&M University 1000 Discovery Drive College Station TX 77845 USA; Guèrin, G.; Borehole Research Group Lamont-Doherty Earth Observatory of Columbia University PO Box 1000, 61 Route 9W Palisades NY 10964 USA; Slagle, A. L.; Borehole Research Group Lamont-Doherty Earth Observatory of Columbia University PO Box 1000, 61 Route 9W Palisades NY 10964 USA; Blair, S. A.; Department of Geological Sciences Florida State University 006 Carraway Building Tallahassee FL 32306 USA; Browne, G. H.; Hydrocarbon Section GNS Science PO Box 30368 Lower Hutt New Zealand; Carter, R. M.; Marine Geophysical Laboratory James Cook University of North Queensland Townsville QLD 4811 Australia; Ciobanu, M.; Laboratoire de Microbiologie des Environnements Extrêmes CNRS UMR-6197 Institut Universitaire Européen de la Mer Technopole Brest-Iroise Plouzane 29280 France; Claypool, G. E.; Organic Geochemist 8910 West 2nd Avenue Lakewood CO 80226 USA; Crundwell, M. P.; New Zealand Observer/Paleontologist (foraminifers) Paleontology and Environmental Change Section GNS Science PO Box 30368 Lower Hutt New Zealand; Dinarès-Turell, J.; Istituto Nazionale di Geofisica e Vulcanologia, Sezione Roma2, Roma, Italia; Ding, X.; School of Marine Sciences China University of Geosciences (Beijing) 29 XueYuan Road, Haidian District Beijing P.R. China; George, S. C.; Department of Earth and Planetary Sciences Macquarie University Sydney NSW 2109 Australia; Hepp, D. A.; MARUM – Center for Marine Environmental Sciences and Department of Geosciences University of Bremen Leobener Strasse MARUM Building, Room 2230 28359 Bremen Germany

    2010-01-01

    Integrated Ocean Drilling Program (IODP) Expedition 317 was devoted to understanding the relative importance of global sea level (eustasy) versus local tectonic and sedimentary processes in controlling continental margin sedimentary cycles. The expedition recovered sediments from the Eocene to recent period, with a particular focus on the sequence stratigraphy of the late Miocene to recent, when global sea level change was dominated by glacioeustasy. Drilling in the Canterbury Basin,...

  4. Testing for Human Immunodeficiency Virus

    Science.gov (United States)

    ... education Fact Sheet PFS005: Testing for Human Immunodeficiency Virus AUGUST 2015 • Reasons for Getting Tested • Who Should ... For More Information • Glossary Testing for Human Immunodeficiency Virus Human immunodeficiency virus (HIV) is the virus that ...

  5. Analysis of Virus Algorithms

    Directory of Open Access Journals (Sweden)

    Jyoti Kalyani

    2006-01-01

    Full Text Available Security of wired and wireless networks is the most challengeable in today's computer world. The aim of this study was to give brief introduction about viruses and worms, their creators and characteristics of algorithms used by viruses. Here wired and wireless network viruses are elaborated. Also viruses are compared with human immune system. On the basis of this comparison four guidelines are given to detect viruses so that more secure systems are made. While concluding this study it is found that the security is most challengeable, thus it is required to make more secure models which automatically detect viruses and prevent the system from its affect.

  6. Hepatitis Delta Virus: A Peculiar Virus

    OpenAIRE

    Carolina Alves; Cristina Branco; Celso Cunha

    2013-01-01

    The hepatitis delta virus (HDV) is distributed worldwide and related to the most severe form of viral hepatitis. HDV is a satellite RNA virus dependent on hepatitis B surface antigens to assemble its envelope and thus form new virions and propagate infection. HDV has a small 1.7 Kb genome making it the smallest known human virus. This deceivingly simple virus has unique biological features and many aspects of its life cycle remain elusive. The present review endeavors to gather the available ...

  7. Virus Infection

    Directory of Open Access Journals (Sweden)

    Hiroshi Abe

    2013-01-01

    Full Text Available Of 168 patients with chronic hepatitis B virus (HBV infection-related liver disease, 20 patients who had received 100 mg of lamivudine plus 10 mg/day of adefovir dipivoxil (ADV (ADV group and 124 patients who had received 0.5 mg/day of entecavir or 100 mg/day of lamivudine (non-ADV group for >1 year were enrolled. For comparative analyses, 19 well-matched pairs were obtained from the groups by propensity scores. At the time of enrollment, serum creatinine and phosphate concentrations were similar between the ADV and non-ADV groups; however, urinary phosphate ( and serum bone-specific alkaline phosphatase (BAP ( concentrations were significantly higher in the ADV group than in the non-ADV group. Serum BAP was significantly higher at the time of enrollment than before ADV administration in the ADV group (, although there was no significant change in serum BAP concentration in the non-ADV group. There was a significant positive correlation between the period of ADV therapy and ΔBAP (, . Serum BAP concentration increased before increase in serum creatinine concentration and was useful for early detection of adverse events and for developing adequate measures for continuing ADV for chronic HBV infection-related liver disease.

  8. Multiple oscillatory modes of the Argentine Basin. Part II. The spectral origin of the basin modes

    NARCIS (Netherlands)

    Weijer, W.; Vevier, F.; Gille, S.T.; Dijkstra, H.A.

    2007-01-01

    In this paper the spectrum of barotropic basin modes of the Argentine Basin is shown to be connected to the classical Rossby basin modes of a flat-bottom (constant depth), rectangular basin. First, the spectrum of basin modes is calculated for the Argentine Basin, by performing a normal-mode analysi

  9. Intracontinental basins and strong earthquakes

    Institute of Scientific and Technical Information of China (English)

    邓起东; 高孟潭; 赵新平; 吴建春

    2004-01-01

    The September 17, 1303 Hongtong M=8 earthquake occurred in Linfen basin of Shanxi down-faulted basin zone. It is the first recorded M=8 earthquake since the Chinese historical seismic records had started and is a great earthquake occurring in the active intracontinental basin. We had held a Meeting of the 700th Anniversary of the 1303 Hongtong M=8 Earthquake in Shanxi and a Symposium on Intracontinental Basins and Strong Earthquakes in Taiyuan City of Shanxi Province on September 17~18, 2003. The articles presented on the symposium discussed the relationships between active intracontinental basins of different properties, developed in different regions, including tensional graben and semi-graben basins in tensile tectonic regions, compression-depression basins and foreland basins in compressive tectonic regions and pull-apart basins in strike-slip tectonic zones, and strong earthquakes in China. In this article we make a brief summary of some problems. The articles published in this special issue are a part of the articles presented on the symposium.

  10. Toscana virus meningitis case in Switzerland: an example of the ezVIR bioinformatics pipeline utility for the identification of emerging viruses.

    Science.gov (United States)

    Cordey, S; Bel, M; Petty, T J; Docquier, M; Sacco, L; Turin, L; Cherpillod, P; Emonet, S; Louis-Simonet, M; Zdobnov, E M; Ambrosioni, J; Kaiser, L

    2015-04-01

    Toscana virus (TOSV) represents a frequent cause of viral meningitis in the Mediterranean Basin that remains neglected in neighbouring countries. We report a documented TOSV meningitis case in a traveller returning from Tuscany to Switzerland. While routine serological and PCR assays could not discriminate between TOSV and Sandfly fever Naples virus infection, a high-throughput sequencing performed directly on the cerebrospinal fluid specimen and analysed with the ezVIR pipeline provided an unequivocal viral diagnostic. TOSV could be unequivocally considered as the aetiological agent, proving the potential of ezVIR to improve standard diagnostics in cases of infection with uncommon or emerging viruses.

  11. Viruses and Breast Cancer

    Directory of Open Access Journals (Sweden)

    James S. Lawson

    2010-04-01

    Full Text Available Viruses are the accepted cause of many important cancers including cancers of the cervix and anogenital area, the liver, some lymphomas, head and neck cancers and indirectly human immunodeficiency virus associated cancers. For over 50 years, there have been serious attempts to identify viruses which may have a role in breast cancer. Despite these efforts, the establishment of conclusive evidence for such a role has been elusive. However, the development of extremely sophisticated new experimental techniques has allowed the recent development of evidence that human papilloma virus, Epstein-Barr virus, mouse mammary tumor virus and bovine leukemia virus may each have a role in the causation of human breast cancers. This is potentially good news as effective vaccines are already available to prevent infections from carcinogenic strains of human papilloma virus, which causes cancer of the uterine cervix.

  12. Tumorigenic DNA viruses

    Energy Technology Data Exchange (ETDEWEB)

    Klein, G.

    1989-01-01

    The eighth volume of Advances in Viral Oncology focuses on the three major DNA virus groups with a postulated or proven tumorigenic potential: papillomaviruses, animal hepatitis viruses, and the Epstein-Bar virus. In the opening chapters, the contributors analyze the evidence that papillomaviruses and animal hepatitis viruses are involved in tumorigenesis and describe the mechanisms that trigger virus-host cell interactions. A detailed section on the Epstein-Barr virus (EBV) - comprising more than half the book - examines the transcription and mRNA processing patterns of the virus genome; the mechanisms by which EBV infects lymphoid and epithelial cells; the immunological aspects of the virus; the actions of EBV in hosts with Acquired Immune Deficiency Syndrome; and the involvement of EBV in the etiology of Burkitt's lymphoma.

  13. Viruses and Breast Cancer

    Energy Technology Data Exchange (ETDEWEB)

    Lawson, James S., E-mail: james.lawson@unsw.edu.au; Heng, Benjamin [School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney (Australia)

    2010-04-30

    Viruses are the accepted cause of many important cancers including cancers of the cervix and anogenital area, the liver, some lymphomas, head and neck cancers and indirectly human immunodeficiency virus associated cancers. For over 50 years, there have been serious attempts to identify viruses which may have a role in breast cancer. Despite these efforts, the establishment of conclusive evidence for such a role has been elusive. However, the development of extremely sophisticated new experimental techniques has allowed the recent development of evidence that human papilloma virus, Epstein-Barr virus, mouse mammary tumor virus and bovine leukemia virus may each have a role in the causation of human breast cancers. This is potentially good news as effective vaccines are already available to prevent infections from carcinogenic strains of human papilloma virus, which causes cancer of the uterine cervix.

  14. Viruses and Breast Cancer

    International Nuclear Information System (INIS)

    Viruses are the accepted cause of many important cancers including cancers of the cervix and anogenital area, the liver, some lymphomas, head and neck cancers and indirectly human immunodeficiency virus associated cancers. For over 50 years, there have been serious attempts to identify viruses which may have a role in breast cancer. Despite these efforts, the establishment of conclusive evidence for such a role has been elusive. However, the development of extremely sophisticated new experimental techniques has allowed the recent development of evidence that human papilloma virus, Epstein-Barr virus, mouse mammary tumor virus and bovine leukemia virus may each have a role in the causation of human breast cancers. This is potentially good news as effective vaccines are already available to prevent infections from carcinogenic strains of human papilloma virus, which causes cancer of the uterine cervix

  15. Herpes Simplex Virus (HSV)

    Science.gov (United States)

    ... rashes clinical tools newsletter | contact Share | Herpes Simplex Virus (HSV) A parent's guide to condition and treatment ... skin or mouth sores with the herpes simplex virus (HSV) is called primary herpes. This may be ...

  16. Hepatitis virus panel

    Science.gov (United States)

    ... page: //medlineplus.gov/ency/article/003558.htm Hepatitis virus panel To use the sharing features on this page, please enable JavaScript. The hepatitis virus panel is a series of blood tests used ...

  17. Human Parainfluenza Viruses

    Science.gov (United States)

    ... Search The CDC Cancel Submit Search The CDC Human Parainfluenza Viruses (HPIVs) Note: Javascript is disabled or ... CDC.gov . Recommend on Facebook Tweet Share Compartir Human parainfluenza viruses (HPIVs) commonly cause respiratory illnesses in ...

  18. Understanding Ebola Virus Transmission

    OpenAIRE

    Seth Judson; Joseph Prescott; Vincent Munster

    2015-01-01

    An unprecedented number of Ebola virus infections among healthcare workers and patients have raised questions about our understanding of Ebola virus transmission. Here, we explore different routes of Ebola virus transmission between people, summarizing the known epidemiological and experimental data. From this data, we expose important gaps in Ebola virus research pertinent to outbreak situations. We further propose experiments and methods of data collection that will enable scientists to fil...

  19. Taming influenza viruses

    OpenAIRE

    Ozawa, Makoto; Kawaoka, Yoshihiro

    2011-01-01

    Plasmid-based reverse genetics systems allow the artificial generation of viruses with cloned cDNA-derived genomes. Since the establishment of such systems for influenza virus, numerous attempts have been made to tame this pathogenic agent. In particular, several types of viruses expressing foreign genes have been generated and used to further our knowledge of influenza virus replication and pathogenicity and to develop novel influenza vaccines. Here, we review these achievements and discuss ...

  20. BOVINE VIRAL DIARRHEA VIRUSES

    Science.gov (United States)

    Bovine viral diarrhea virus (BVDV) is an umbrella term for two species of viruses, BVDV1 and BVDV2, within the Pestivirus genus of the Flavivirus family. BVDV viruses are further subclassified as cytopathic and noncytopathic based on their activity in cultured epithelial cells. Noncytopathic BVDV p...

  1. Computer Virus Protection

    Science.gov (United States)

    Rajala, Judith B.

    2004-01-01

    A computer virus is a program--a piece of executable code--that has the unique ability to replicate. Like biological viruses, computer viruses can spread quickly and are often difficult to eradicate. They can attach themselves to just about any type of file, and are spread by replicating and being sent from one individual to another. Simply having…

  2. What's West Nile Virus?

    Science.gov (United States)

    ... Help White House Lunch Recipes What's West Nile Virus? KidsHealth > For Kids > What's West Nile Virus? Print A A A Text Size en español ¿Qué es el Virus del Nilo Occidental? What exactly is the West ...

  3. Respiratory Syncytial Virus

    Science.gov (United States)

    ... Things to Know About Zika & Pregnancy Respiratory Syncytial Virus KidsHealth > For Parents > Respiratory Syncytial Virus Print A A A Text Size What's in ... RSV When to Call the Doctor en español Virus respiratorio sincitial About RSV Respiratory syncytial (sin-SISH- ...

  4. Virus diseases of fish

    Science.gov (United States)

    Watson, Stanley W.

    1954-01-01

    Viruses are probably the cause of a wide spectrum of fish diseases. Although relatively few virus diseases of fish are known today, some of the diseases of unknown etiology, as well as some diseases presently accepted as due to bacteria, protozoa, fungi or nutritional deficiencies, possibly will be recognized eventually as virus diseases.

  5. The taxonomy of viruses should include viruses.

    Science.gov (United States)

    Calisher, Charles H

    2016-05-01

    Having lost sight of its goal, the International Committee on Taxonomy of Viruses has redoubled its efforts. That goal is to arrive at a consensus regarding virus classification, i.e., proper placement of viruses in a hierarchical taxonomic scheme; not an easy task given the wide variety of recognized viruses. Rather than suggesting a continuation of the bureaucratic machinations of the past, this opinion piece is a call for insertion of common sense in sorting out the avalanche of information already, and soon-to-be, accrued data. In this way information about viruses ideally would be taxonomically correct as well as useful to working virologists and journal editors, rather than being lost, minimized, or ignored.

  6. Advanced Chemistry Basins Model

    Energy Technology Data Exchange (ETDEWEB)

    Blanco, Mario; Cathles, Lawrence; Manhardt, Paul; Meulbroek, Peter; Tang, Yongchun

    2003-02-13

    The objective of this project is to: (1) Develop a database of additional and better maturity indicators for paleo-heat flow calibration; (2) Develop maturation models capable of predicting the chemical composition of hydrocarbons produced by a specific kerogen as a function of maturity, heating rate, etc.; assemble a compositional kinetic database of representative kerogens; (3) Develop a 4 phase equation of state-flash model that can define the physical properties (viscosity, density, etc.) of the products of kerogen maturation, and phase transitions that occur along secondary migration pathways; (4) Build a conventional basin model and incorporate new maturity indicators and data bases in a user-friendly way; (5) Develop an algorithm which combines the volume change and viscosities of the compositional maturation model to predict the chemistry of the hydrocarbons that will be expelled from the kerogen to the secondary migration pathways; (6) Develop an algorithm that predicts the flow of hydrocarbons along secondary migration pathways, accounts for mixing of miscible hydrocarbon components along the pathway, and calculates the phase fractionation that will occur as the hydrocarbons move upward down the geothermal and fluid pressure gradients in the basin; and (7) Integrate the above components into a functional model implemented on a PC or low cost workstation.

  7. Viruses of asparagus.

    Science.gov (United States)

    Tomassoli, Laura; Tiberini, Antonio; Vetten, Heinrich-Josef

    2012-01-01

    The current knowledge on viruses infecting asparagus (Asparagus officinalis) is reviewed. Over half a century, nine virus species belonging to the genera Ilarvirus, Cucumovirus, Nepovirus, Tobamovirus, Potexvirus, and Potyvirus have been found in this crop. The potyvirus Asparagus virus 1 (AV1) and the ilarvirus Asparagus virus 2 (AV2) are widespread and negatively affect the economic life of asparagus crops reducing yield and increasing the susceptibility to biotic and abiotic stress. The main properties and epidemiology of AV1 and AV2 as well as diagnostic techniques for their detection and identification are described. Minor viruses and control are briefly outlined.

  8. Understanding Ebola Virus Transmission

    Directory of Open Access Journals (Sweden)

    Seth Judson

    2015-02-01

    Full Text Available An unprecedented number of Ebola virus infections among healthcare workers and patients have raised questions about our understanding of Ebola virus transmission. Here, we explore different routes of Ebola virus transmission between people, summarizing the known epidemiological and experimental data. From this data, we expose important gaps in Ebola virus research pertinent to outbreak situations. We further propose experiments and methods of data collection that will enable scientists to fill these voids in our knowledge about the transmission of Ebola virus.

  9. Viruses of asparagus.

    Science.gov (United States)

    Tomassoli, Laura; Tiberini, Antonio; Vetten, Heinrich-Josef

    2012-01-01

    The current knowledge on viruses infecting asparagus (Asparagus officinalis) is reviewed. Over half a century, nine virus species belonging to the genera Ilarvirus, Cucumovirus, Nepovirus, Tobamovirus, Potexvirus, and Potyvirus have been found in this crop. The potyvirus Asparagus virus 1 (AV1) and the ilarvirus Asparagus virus 2 (AV2) are widespread and negatively affect the economic life of asparagus crops reducing yield and increasing the susceptibility to biotic and abiotic stress. The main properties and epidemiology of AV1 and AV2 as well as diagnostic techniques for their detection and identification are described. Minor viruses and control are briefly outlined. PMID:22682173

  10. Online identification of viruses.

    Science.gov (United States)

    Kolaskar, A S; Naik, P S

    2000-06-01

    A computerized animal virus information system is developed in the Sequence Retrieval System (SRS) format. This database is available on the Word Wide Web (WWW) at the site http://bioinfo.ernet.in/www/avis/avis++ +.html. The database has been used to generate large number of identification matrices for each family. The software is developed in C. Unix shell scripts and Hypertext Marked-up Language (HTML) to assign the family to an unknown virus deterministically and to identify the virus probabilistically. It has been shown that such web based virus identification approach provides results with high confidence in those cases where identification matrix uses large number of independent characters. Protein sequence data for animal viruses have been analyzed and oligopeptides specific to each virus family and also specific to each virus species are identified for several viruses. These peptides thus could be used to identify the virus and to assign the virus family with high confidence showing the usefulness of sequence data in virus identification. PMID:10917875

  11. K-Basins design guidelines

    Energy Technology Data Exchange (ETDEWEB)

    Roe, N.R.; Mills, W.C.

    1995-06-01

    The purpose of the design guidelines is to enable SNF and K Basin personnel to complete fuel and sludge removal, and basin water mitigation by providing engineering guidance for equipment design for the fuel basin, facility modifications (upgrades), remote tools, and new processes. It is not intended to be a purchase order reference for vendors. The document identifies materials, methods, and components that work at K Basins; it also Provides design input and a technical review process to facilitate project interfaces with operations in K Basins. This document is intended to compliment other engineering documentation used at K Basins and throughout the Spent Nuclear Fuel Project. Significant provisions, which are incorporated, include portions of the following: General Design Criteria (DOE 1989), Standard Engineering Practices (WHC-CM-6-1), Engineering Practices Guidelines (WHC 1994b), Hanford Plant Standards (DOE-RL 1989), Safety Analysis Manual (WHC-CM-4-46), and Radiological Design Guide (WHC 1994f). Documents (requirements) essential to the engineering design projects at K Basins are referenced in the guidelines.

  12. K-Basins design guidelines

    International Nuclear Information System (INIS)

    The purpose of the design guidelines is to enable SNF and K Basin personnel to complete fuel and sludge removal, and basin water mitigation by providing engineering guidance for equipment design for the fuel basin, facility modifications (upgrades), remote tools, and new processes. It is not intended to be a purchase order reference for vendors. The document identifies materials, methods, and components that work at K Basins; it also Provides design input and a technical review process to facilitate project interfaces with operations in K Basins. This document is intended to compliment other engineering documentation used at K Basins and throughout the Spent Nuclear Fuel Project. Significant provisions, which are incorporated, include portions of the following: General Design Criteria (DOE 1989), Standard Engineering Practices (WHC-CM-6-1), Engineering Practices Guidelines (WHC 1994b), Hanford Plant Standards (DOE-RL 1989), Safety Analysis Manual (WHC-CM-4-46), and Radiological Design Guide (WHC 1994f). Documents (requirements) essential to the engineering design projects at K Basins are referenced in the guidelines

  13. Postmortem stability of Ebola virus.

    Science.gov (United States)

    Prescott, Joseph; Bushmaker, Trenton; Fischer, Robert; Miazgowicz, Kerri; Judson, Seth; Munster, Vincent J

    2015-05-01

    The ongoing Ebola virus outbreak in West Africa has highlighted questions regarding stability of the virus and detection of RNA from corpses. We used Ebola virus-infected macaques to model humans who died of Ebola virus disease. Viable virus was isolated <7 days posteuthanasia; viral RNA was detectable for 10 weeks.

  14. Marketing San Juan Basin gas

    International Nuclear Information System (INIS)

    Marketing natural gas produced in the San Juan Basin of New Mexico and Colorado principally involves four gas pipeline companies with significant facilities in the basin. The system capacity, transportation rates, regulatory status, and market access of each of these companies is evaluated. Because of excess gas supplies available to these pipeline companies, producers can expect improved take levels and prices by selling gas directly to end users and utilities as opposed to selling gas to the pipelines for system supply. The complexities of transporting gas today suggest that the services of an independent gas marketing company may be beneficial to smaller producers with gas supplies in the San Juan Basin

  15. Constructing computer virus phylogenies

    Energy Technology Data Exchange (ETDEWEB)

    Goldberg, L.A. [Warwick Univ., Coventry (United Kingdom) Dept. of Computer Science; Goldberg, P.W. [Aston Univ., Birmingham (United Kingdom) Dept. of Applied Mathematics; Phillips, C.A. [Sandia National Labs., Albuquerque, NM (United States); Sorkin, G.B. [International Business Machines Corp., Yorktown Heights, NY (United States). Thomas J. Watson Research Center

    1996-03-01

    There has been much recent algorithmic work on the problem of reconstructing the evolutionary history of biological species. Computer virus specialists are interested in finding the evolutionary history of computer viruses--a virus is often written using code fragments from one or more other viruses, which are its immediate ancestors. A phylogeny for a collection of computer viruses is a directed acyclic graph whose nodes are the viruses and whose edges map ancestors to descendants and satisfy the property that each code fragment is ``invented`` only once. To provide a simple explanation for the data, we consider the problem of constructing such a phylogeny with a minimal number of edges. In general, this optimization problem cannot be solved in quasi-polynomial time unless NQP=QP; we present positive and negative results for associated approximated problems. When tree solutions exist, they can be constructed and randomly sampled in polynomial time.

  16. Sulfated polysaccharides are potent and selective inhibitors of various enveloped viruses, including herpes simplex virus, cytomegalovirus, vesicular stomatitis virus, and human immunodeficiency virus.

    OpenAIRE

    Baba, M.; Snoeck, R; Pauwels, R; De Clercq, E

    1988-01-01

    Several sulfated polysaccharides (dextran sulfate, pentosan polysulfate, fucoidan, and carrageenans) proved to be potent inhibitors for herpes simplex virus, human cytomegalovirus, vesicular stomatitis virus, Sindbis virus, and human immunodeficiency virus. They were moderately inhibitory to vaccinia virus but not inhibitory to adenovirus, coxsackievirus, poliovirus, parainfluenza virus, and reovirus. These results indicate that, with the exception of parainfluenza virus, enveloped viruses ar...

  17. The human oncogenic viruses

    Energy Technology Data Exchange (ETDEWEB)

    Luderer, A.A.; Weetall, H.H

    1986-01-01

    This book contains eight selections. The titles are: Cytogenetics of the Leukemias and Lymphomas; Cytogenetics of Solid Tumors: Renal Cell Carcinoma, Malignant Melanoma, Retinoblastoma, and Wilms' Tumor; Elucidation of a Normal Function for a Human Proto-Oncogene; Detection of HSV-2 Genes and Gene Products in Cervical Neoplasia; Papillomaviruses in Anogennital Neoplasms; Human Epstein-Barr Virus and Cancer; Hepatitis B Virus and Hepatocellular Carcinoma; and Kaposi's Sarcoma: Acquired Immunodeficiency Syndrome (AIDS) and Associated Viruses.

  18. The Influenza Virus Enigma

    OpenAIRE

    Salomon, Rachelle; Webster, Robert G.

    2009-01-01

    Both seasonal and pandemic influenza continue to challenge both scientists and clinicians. Drug-resistant H1N1 influenza viruses have dominated the 2009 flu season, and the H5N1 avian influenza virus continues to kill both people and poultry in Eurasia. Here, we discuss the pathogenesis and transmissibility of influenza viruses and we emphasize the need to find better predictors of both seasonal and potentially pandemic influenza.

  19. Endocytosis of influenza viruses

    OpenAIRE

    Lakadamyali, Melike; Rust, Michael J.; Zhuang, Xiaowei

    2004-01-01

    Receptor-mediated endocytosis is known to play an important role in the entry of many viruses into host cells. However, the exact internalization mechanism has, until recently, remained poorly understood for many medically important viruses, including influenza. Developments in real-time imaging of single viruses as well as the use of dominant negative mutants to selectively block specific endocytic pathways, have improved our understanding of the influenza infection process.

  20. Filamentous influenza viruses

    OpenAIRE

    Dadonaite, Bernadeta; Vijyakrishnan, Swetha; Fodor, Ervin; Bhella, David; Hutchinson, Edward C.

    2016-01-01

    Clinical isolates of influenza virus produce pleomorphic virus particles, including extremely long filamentous virions. In contrast, strains of influenza that have adapted to laboratory growth typically produce only spherical virions. As a result, the filamentous phenotype has been overlooked in most influenza virus research. Recent advances in imaging and improved animal models have highlighted the distinct structure and functional relevance of filamentous virions. In this review we summaris...

  1. The virus of management

    OpenAIRE

    Kjær, Peter; Frankel, Christian

    2004-01-01

    The virus metaphor may be used in studies of management knowledge not only as a way of describing diffusion processes but also as a way of thinking about viral elements of knowledge production. In the present article, organizational viruses are viewed as ensembles of basic distinctions that are constitutive of concrete bodies of knowledge and which form mutable engines of organizational self-descriptions. Organizational viruses, we contend, are both characterized by stability i...

  2. Allegheny County Basin Outlines Map

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — This basins dataset was created to initiate regional watershed approaches with respect to sewer rehabilitation. If viewing this description on the Western...

  3. Trip report Rainwater Basin Nebraska

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This report is a summary a trip to Rainwater Basin Wetland Management District in 1991, and focuses on the hydrology and soil habitat types. It is part of the...

  4. FAQ: General Questions about West Nile Virus

    Science.gov (United States)

    ... Public Service Videos General Questions About West Nile Virus Recommend on Facebook Tweet Share Compartir On this ... West Nile virus cases? What is West Nile virus? West Nile virus is an arthropod-borne virus ( ...

  5. 77 FR 45653 - Yakima River Basin Conservation Advisory Group; Yakima River Basin Water Enhancement Project...

    Science.gov (United States)

    2012-08-01

    ... Bureau of Reclamation Yakima River Basin Conservation Advisory Group; Yakima River Basin Water... on the structure, implementation, and oversight of the Yakima River Basin Water Conservation Program... of the Water Conservation Program, including the applicable water conservation guidelines of...

  6. RESERVES IN WESTERN BASINS PART IV: WIND RIVER BASIN

    Energy Technology Data Exchange (ETDEWEB)

    Robert Caldwell

    1998-04-01

    Vast quantities of natural gas are entrapped within various tight formations in the Rocky Mountain area. This report seeks to quantify what proportion of that resource can be considered recoverable under today's technological and economic conditions and discusses factors controlling recovery. The ultimate goal of this project is to encourage development of tight gas reserves by industry through reducing the technical and economic risks of locating, drilling and completing commercial tight gas wells. This report is the fourth in a series and focuses on the Wind River Basin located in west central Wyoming. The first three reports presented analyses of the tight gas reserves and resources in the Greater Green River Basin (Scotia, 1993), Piceance Basin (Scotia, 1995) and the Uinta Basin (Scotia, 1995). Since each report is a stand-alone document, duplication of language will exist where common aspects are discussed. This study, and the previous three, describe basin-centered gas deposits (Masters, 1979) which contain vast quantities of natural gas entrapped in low permeability (tight), overpressured sandstones occupying a central basin location. Such deposits are generally continuous and are not conventionally trapped by a structural or stratigraphic seal. Rather, the tight character of the reservoirs prevents rapid migration of the gas, and where rates of gas generation exceed rates of escape, an overpressured basin-centered gas deposit results (Spencer, 1987). Since the temperature is a primary controlling factor for the onset and rate of gas generation, these deposits exist in the deeper, central parts of a basin where temperatures generally exceed 200 F and drill depths exceed 8,000 feet. The abbreviation OPT (overpressured tight) is used when referring to sandstone reservoirs that comprise the basin-centered gas deposit. Because the gas resources trapped in this setting are so large, they represent an important source of future gas supply, prompting studies

  7. Virus separation using membranes.

    Science.gov (United States)

    Grein, Tanja A; Michalsky, Ronald; Czermak, Peter

    2014-01-01

    Industrial manufacturing of cell culture-derived viruses or virus-like particles for gene therapy or vaccine production are complex multistep processes. In addition to the bioreactor, such processes require a multitude of downstream unit operations for product separation, concentration, or purification. Similarly, before a biopharmaceutical product can enter the market, removal or inactivation of potential viral contamination has to be demonstrated. Given the complexity of biological solutions and the high standards on composition and purity of biopharmaceuticals, downstream processing is the bottleneck in many biotechnological production trains. Membrane-based filtration can be an economically attractive and efficient technology for virus separation. Viral clearance, for instance, of up to seven orders of magnitude has been reported for state of the art polymeric membranes under best conditions.This chapter summarizes the fundamentals of virus ultrafiltration, diafiltration, or purification with adsorptive membranes. In lieu of an impractical universally applicable protocol for virus filtration, application of these principles is demonstrated with two examples. The chapter provides detailed methods for production, concentration, purification, and removal of a rod-shaped baculovirus (Autographa californica M nucleopolyhedrovirus, about 40 × 300 nm in size, a potential vector for gene therapy, and an industrially important protein expression system) or a spherical parvovirus (minute virus of mice, 22-26 nm in size, a model virus for virus clearance validation studies).

  8. Rift Valley Fever Virus

    Science.gov (United States)

    Rift Valley fever virus (RVFV) is a mosquito-transmitted virus or arbovirus that is endemic in sub-Saharan Africa. In the last decade, Rift Valley fever (RVF) outbreaks have resulted in loss of human and animal life, as well as had significant economic impact. The disease in livestock is primarily a...

  9. Viral Haemorrhagic Septicaemia Virus

    DEFF Research Database (Denmark)

    Olesen, Niels Jørgen; Skall, Helle Frank

    2013-01-01

    This chapter covers the genetics (genotypes and serotypes), clinical signs, host species, transmission, prevalence, diagnosis, control and prevention of viral haemorrhagic septicaemia virus.......This chapter covers the genetics (genotypes and serotypes), clinical signs, host species, transmission, prevalence, diagnosis, control and prevention of viral haemorrhagic septicaemia virus....

  10. INFLUENZA VIRUS IN POULTRY

    Science.gov (United States)

    Avian influenza virus (AIV) is normally found in wild birds, particularly in ducks and shorebirds, where it does not cause any perceptible clinical disease. However, poultry, including chickens and turkeys, are not normal hosts for avian influenza, but if the virus is introduced it can result in mi...

  11. Zika Virus and Pregnancy.

    Science.gov (United States)

    Stagg, Denise; Hurst, Helen M

    2016-01-01

    Recent outbreaks of Zika virus and reports linking infection in pregnant women with microcephaly in newborns have caused concern worldwide. Information has been evolving rapidly. Nurses and other clinicians, especially those who work with women of childbearing age, play a pivotal role in disseminating accurate information and identifying potential cases of Zika virus infection. PMID:27287356

  12. Positive reinforcement for viruses

    OpenAIRE

    Vigant, Frederic; Jung, Michael; Lee, Benhur

    2010-01-01

    Virus-cell membrane fusion requires a critical transition from positive to negative membrane curvature. St. Vincent et al., in PNAS (St Vincent, et al., 2010), designed a class of antivirals that targets this transition. These Rigid Amphipathic Fusion Inhibitors are active against an array of enveloped viruses.

  13. Positive reinforcement for viruses.

    Science.gov (United States)

    Vigant, Frederic; Jung, Michael; Lee, Benhur

    2010-10-29

    Virus-cell membrane fusion requires a critical transition from positive to negative membrane curvature. St. Vincent et al. (2010), in PNAS, designed a class of antivirals that targets this transition. These rigid amphipathic fusion inhibitors are active against an array of enveloped viruses.

  14. Water Accounting from Ungauged Basins

    Science.gov (United States)

    Bastiaanssen, W. G.; Savenije, H.

    2014-12-01

    Water scarcity is increasing globally. This requires a more accurate management of the water resources at river basin scale and understanding of withdrawals and return flows; both naturally and man-induced. Many basins and their tributaries are, however, ungauged or poorly gauged. This hampers sound planning and monitoring processes. While certain countries have developed clear guidelines and policies on data observatories and data sharing, other countries and their basin organization still have to start on developing data democracies. Water accounting quantifies flows, fluxes, stocks and consumptive use pertaining to every land use class in a river basin. The objective is to derive a knowledge base with certain minimum information that facilitates decision making. Water Accounting Plus (WA+) is a new method for water resources assessment reporting (www.wateraccounting.org). While the PUB framework has yielded several deterministic models for flow prediction, WA+ utilizes remote sensing data of rainfall, evaporation (including soil, water, vegetation and interception evaporation), soil moisture, water levels, land use and biomass production. Examples will be demonstrated that show how remote sensing and hydrological models can be smartly integrated for generating all the required input data into WA+. A standard water accounting system for all basins in the world - with a special emphasis on data scarce regions - is under development. First results of using remote sensing measurements and hydrological modeling as an alternative to expensive field data sets, will be presented and discussed.

  15. Computer Viruses: Pathology and Detection.

    Science.gov (United States)

    Maxwell, John R.; Lamon, William E.

    1992-01-01

    Explains how computer viruses were originally created, how a computer can become infected by a virus, how viruses operate, symptoms that indicate a computer is infected, how to detect and remove viruses, and how to prevent a reinfection. A sidebar lists eight antivirus resources. (four references) (LRW)

  16. Postmortem Stability of Ebola Virus

    OpenAIRE

    Prescott, Joseph; Bushmaker, Trenton; Fischer, Robert; Miazgowicz, Kerri; Judson, Seth; Vincent J. Munster

    2015-01-01

    The ongoing Ebola virus outbreak in West Africa has highlighted questions regarding stability of the virus and detection of RNA from corpses. We used Ebola virus–infected macaques to model humans who died of Ebola virus disease. Viable virus was isolated

  17. Polyoma BK Virus: An Oncogenic Virus?

    Directory of Open Access Journals (Sweden)

    Syed Hassan

    2013-01-01

    Full Text Available We report a case of a 65-year-old gentleman with a history of end stage renal disease who underwent a successful cadaveric donor kidney transplant four years ago. He subsequently developed BK virus nephropathy related to chronic immunosuppressant therapy. Three years later, misfortune struck again, and he developed adenocarcinoma of the bladder.

  18. Geology, exploration status of Uruguay's sedimentary basins

    Energy Technology Data Exchange (ETDEWEB)

    Goso, C.; Santa Ana, H. de (Administracion Nacional de Combustibles, Alcohol y Portland (Uruguay))

    1994-02-07

    This article attempts to present the geological characteristics and tectonic and sedimentary evolution of Uruguayan basins and the extent to which they have been explored. Uruguay is on the Atlantic coast of South America. The country covers about 318,000 sq km, including offshore and onshore territories corresponding to more than 65% of the various sedimentary basins. Four basins underlie the country: the Norte basin, the Santa Lucia basin, the offshore Punta del Este basin, and the offshore-onshore Pelotas-Merin basin. The Norte basin is a Paleozoic basin while the others are Mesozoic basins. Each basin has been explored to a different extent, as this paper explains.

  19. WATSTORE Stream Flow Basin Characteristics File

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — The Stream Flow Basin Characteristics file contains information about the drainage basins of selected USGS gaging stations. Data elements of this file were...

  20. Origin of the earth's ocean basins

    Science.gov (United States)

    Frey, H.

    1977-01-01

    The earth's original ocean basins are proposed to be mare-type basins produced 4 billion y.a. by the flux of asteroid-sized objects responsible for the lunar mare basins. Scaling upward from the observed number of lunar basins for the greater capture cross-section and impact velocity of the earth indicates that at least 50% of an original global crust would have been converted to basin topography. These basins were flooded by basaltic liquids in times short compared to the isostatic adjustment time for the basin. The modern crustal dichotomy (60% oceanic, 40% continental crust) was established early in the history of the earth, making possible the later onset of plate tectonic processes. These later processes have subsequently reworked, in several cycles, principally the oceanic parts of the earth's crust, changing the configuration of the continents in the process. Ocean basins (and oceans themselves) may be rare occurrences on planets in other star systems.

  1. [Ebola virus disease].

    Science.gov (United States)

    Nazimek, Katarzyna; Bociaga-Jasik, Monika; Bryniarski, Krzysztof; Gałas, Aleksander; Garlicki, Aleksander; Gawda, Anna; Gawlik, Grzegorz; Gil, Krzysztof; Kosz-Vnenchak, Magdalena; Mrozek-Budzyn, Dorota; Olszanecki, Rafał; Piatek, Anna; Zawilińska, Barbara; Marcinkiewicz, Janusz

    2014-01-01

    Ebola is one of the most virulent zoonotic RNA viruses causing in humans haemorrhagic fever with fatality ratio reaching 90%. During the outbreak of 2014 the number of deaths exceeded 8.000. The "imported" cases reported in Western Europe and USA highlighted the extreme risk of Ebola virus spreading outside the African countries. Thus, haemorrhagic fever outbreak is an international epidemiological problem, also due to the lack of approved prevention and therapeutic strategies. The editorial review article briefly summarizes current knowledge on Ebola virus disease epidemiology, etiology, pathogenesis, clinical presentation, diagnosis as well as possible prevention and treatment.

  2. Viruses in reptiles

    Directory of Open Access Journals (Sweden)

    Ariel Ellen

    2011-09-01

    Full Text Available Abstract The etiology of reptilian viral diseases can be attributed to a wide range of viruses occurring across different genera and families. Thirty to forty years ago, studies of viruses in reptiles focused mainly on the zoonotic potential of arboviruses in reptiles and much effort went into surveys and challenge trials of a range of reptiles with eastern and western equine encephalitis as well as Japanese encephalitis viruses. In the past decade, outbreaks of infection with West Nile virus in human populations and in farmed alligators in the USA has seen the research emphasis placed on the issue of reptiles, particularly crocodiles and alligators, being susceptible to, and reservoirs for, this serious zoonotic disease. Although there are many recognised reptilian viruses, the evidence for those being primary pathogens is relatively limited. Transmission studies establishing pathogenicity and cofactors are likewise scarce, possibly due to the relatively low commercial importance of reptiles, difficulties with the availability of animals and permits for statistically sound experiments, difficulties with housing of reptiles in an experimental setting or the inability to propagate some viruses in cell culture to sufficient titres for transmission studies. Viruses as causes of direct loss of threatened species, such as the chelonid fibropapilloma associated herpesvirus and ranaviruses in farmed and wild tortoises and turtles, have re-focused attention back to the characterisation of the viruses as well as diagnosis and pathogenesis in the host itself. 1. Introduction 2. Methods for working with reptilian viruses 3. Reptilian viruses described by virus families 3.1. Herpesviridae 3.2. Iridoviridae 3.2.1 Ranavirus 3.2.2 Erythrocytic virus 3.2.3 Iridovirus 3.3. Poxviridae 3.4. Adenoviridae 3.5. Papillomaviridae 3.6. Parvoviridae 3.7. Reoviridae 3.8. Retroviridae and inclusion body disease of Boid snakes 3.9. Arboviruses 3.9.1. Flaviviridae 3

  3. Ebola (Ebola Virus Disease)

    Science.gov (United States)

    ... Search The CDC Cancel Submit Search The CDC Ebola (Ebola Virus Disease) Note: Javascript is disabled or is ... Tweet Share Compartir CDC's Ongoing Work to Contain Ebola in West Africa The Road to Zero: CDC’s ...

  4. Zika Virus Fact Sheet

    Science.gov (United States)

    ... medical care and advice. There is currently no vaccine available. Prevention Mosquito bites Protection against mosquito bites is a key measure to prevent Zika virus infection. This can be done by wearing ...

  5. Ebola Virus Disease

    Science.gov (United States)

    ... long as their blood contains the virus. Sexual transmission More surveillance data and research are needed on the risks of sexual transmission, and particularly on the prevalence of viable and ...

  6. Hepatitis G virus

    Institute of Scientific and Technical Information of China (English)

    Vasiliy Ivanovich Reshetnyak; Tatiana Igorevna Karlovich; Ljudmila Urievna Ilchenko

    2008-01-01

    A number of new hepatitis viruses (G,TT,SEN) were discovered late in the past century.We review the data available in the literature and our own findings suggesting that the new hepatitis G virus (HGV),disclosed in the late 1990s,has been rather well studied.Analysis of many studies dealing with HGV mainly suggests the lymphotropicity of this virus.HGV or GBV-C has been ascertained to influence course and prognosis in the HIV-infected patient.Until now,the frequent presence of GBV-C in coinfections,hematological diseases,and biliary pathology gives no grounds to determine it as an "accidental tourist" that is of no significance.The similarity in properties of GBV-C and hepatitis C virus (HCV) offers the possibility of using HGV,and its induced experimental infection,as a model to study hepatitis C and to develop a hepatitis C vaccine.

  7. Avoiding Computer Viruses.

    Science.gov (United States)

    Rowe, Joyce; And Others

    1989-01-01

    The threat of computer sabotage is a real concern to business teachers and others responsible for academic computer facilities. Teachers can minimize the possibility. Eight suggestions for avoiding computer viruses are given. (JOW)

  8. Hepatitis B virus (image)

    Science.gov (United States)

    Hepatitis B is also known as serum hepatitis and is spread through blood and sexual contact. It is ... population. This photograph is an electronmicroscopic image of hepatitis B virus particles. (Image courtesy of the Centers for ...

  9. New bifurcations of basin boundaries involving Wada and a smooth Wada basin boundary

    Institute of Scientific and Technical Information of China (English)

    Zou Hai-Lin; Xu Jian-Xue; Jiang Jun

    2008-01-01

    This paper demonstrates and analyses double heteroclinic tangency in a three-well potential model,which can produce three new types of bifurcations of basin boundaries including from smooth to Wada basin boundaries,from fractal to Wada basin boundaries in which no changes of accessible periodic orbits happen,and from Wada to Wada basin boundaries.In a model of mechanical oscillator,it shows that a Wada basin boundary can be smooth.

  10. Ebola Virus Disease

    OpenAIRE

    PV Bhargavan; PV Shiji; Jare Jagannath Udhavrao; Nagaraj Desai

    2015-01-01

    Ebola virus is named after the river in the former Zaire where a haemorrhagic fever initially identified in 1976 involved human to human transmission, as well as spread by contaminated injection equipments. Ebola virus causes an acute febrile illness associated with a high mortality rate. The illness is characterized by multi-system involvement that begins with abrupt onset of headache, myalgia, fever and proceeds to prostration, rash, shock and bleeding manifestation.

  11. Zika Virus Outside Africa

    OpenAIRE

    Hayes, Edward B.

    2009-01-01

    Zika virus (ZIKV) is a flavivirus related to yellow fever, dengue, West Nile, and Japanese encephalitis viruses. In 2007 ZIKV caused an outbreak of relatively mild disease characterized by rash, arthralgia, and conjunctivitis on Yap Island in the southwestern Pacific Ocean. This was the first time that ZIKV was detected outside of Africa and Asia. The history, transmission dynamics, virology, and clinical manifestations of ZIKV disease are discussed, along with the possibility for diagnostic ...

  12. Genome packaging in viruses

    OpenAIRE

    Sun, Siyang; Rao, Venigalla B.; Rossmann, Michael G.

    2010-01-01

    Genome packaging is a fundamental process in a viral life cycle. Many viruses assemble preformed capsids into which the genomic material is subsequently packaged. These viruses use a packaging motor protein that is driven by the hydrolysis of ATP to condense the nucleic acids into a confined space. How these motor proteins package viral genomes had been poorly understood until recently, when a few X-ray crystal structures and cryo-electron microscopy structures became available. Here we discu...

  13. Respiratory Syncytial Virus (RSV)

    Centers for Disease Control (CDC) Podcasts

    2013-02-04

    Respiratory Syncytial Virus, or RSV, causes cold-like symptoms but can be serious for infants and older adults. In this podcast, CDC’s Dr. Eileen Schneider discusses this common virus and offers tips to prevent its spread.  Created: 2/4/2013 by National Center for Immunization and Respiratory Diseases (NCIRD), Division of Viral Diseases (DVD).   Date Released: 2/13/2013.

  14. BASIN: Beowulf Analysis Symbolic INterface

    Science.gov (United States)

    Vesperini, Enrico; Goldberg, David M.; McMillan, Stephen L. W.; Dura, James; Jones, Douglas

    2013-08-01

    BASIN (Beowulf Analysis Symbolic INterface) is a flexible, integrated suite of tools for multiuser parallel data analysis and visualization that allows researchers to harness the power of Beowulf PC clusters and multi-processor machines without necessarily being experts in parallel programming. It also includes general tools for data distribution and parallel operations on distributed data for developing libraries for specific tasks.

  15. Coastal inlets and tidal basins

    NARCIS (Netherlands)

    De Vriend, H.J.; Dronkers, J.; Stive, M.J.F.; Van Dongeren, A.; Wang, J.H.

    2002-01-01

    lecture note: Tidal inlets and their associated basins (lagoons) are a common feature of lowland coasts all around the world. A significant part ofthe world's coastlines is formed by barrier island coasts, and most other tidal coasts are interrupted by estuaries and lagoon inlets. These tidal system

  16. Virus templated metallic nanoparticles

    Science.gov (United States)

    Aljabali, Alaa A. A.; Barclay, J. Elaine; Lomonossoff, George P.; Evans, David J.

    2010-12-01

    Plant viruses are considered as nanobuilding blocks that can be used as synthons or templates for novel materials. Cowpea mosaic virus (CPMV) particles have been shown to template the fabrication of metallic nanoparticles by an electroless deposition metallization process. Palladium ions were electrostatically bound to the virus capsid and, when reduced, acted as nucleation sites for the subsequent metal deposition from solution. The method, although simple, produced highly monodisperse metallic nanoparticles with a diameter of ca. templated particles were prepared with cobalt, nickel, iron, platinum, cobalt-platinum and nickel-iron.Plant viruses are considered as nanobuilding blocks that can be used as synthons or templates for novel materials. Cowpea mosaic virus (CPMV) particles have been shown to template the fabrication of metallic nanoparticles by an electroless deposition metallization process. Palladium ions were electrostatically bound to the virus capsid and, when reduced, acted as nucleation sites for the subsequent metal deposition from solution. The method, although simple, produced highly monodisperse metallic nanoparticles with a diameter of ca. templated particles were prepared with cobalt, nickel, iron, platinum, cobalt-platinum and nickel-iron. Electronic supplementary information (ESI) available: Additional experimental detail, agarose gel electrophoresis results, energy dispersive X-ray spectra, ζ-potential measurements, dynamic light scattering data, nanoparticle tracking analysis and an atomic force microscopy image of Ni-CPMV. See DOI: 10.1039/c0nr00525h

  17. Chikungunya virus and its mosquito vectors.

    Science.gov (United States)

    Higgs, Stephen; Vanlandingham, Dana

    2015-04-01

    Chikungunya virus (CHIKV), a mosquito-borne alphavirus of increasing public health significance, has caused large epidemics in Africa and the Indian Ocean basin; now it is spreading throughout the Americas. The primary vectors of CHIKV are Aedes (Ae.) aegypti and, after the introduction of a mutation in the E1 envelope protein gene, the highly anthropophilic and geographically widespread Ae. albopictus mosquito. We review here research efforts to characterize the viral genetic basis of mosquito-vector interactions, the use of RNA interference and other strategies for the control of CHIKV in mosquitoes, and the potentiation of CHIKV infection by mosquito saliva. Over the past decade, CHIKV has emerged on a truly global scale. Since 2013, CHIKV transmission has been reported throughout the Caribbean region, in North America, and in Central and South American countries, including Brazil, Columbia, Costa Rica, El Salvador, French Guiana, Guatemala, Guyana, Nicaragua, Panama, Suriname, and Venezuela. Closing the gaps in our knowledge of driving factors behind the rapid geographic expansion of CHIKV should be considered a research priority. The abundance of multiple primate species in many of these countries, together with species of mosquito that have never been exposed to CHIKV, may provide opportunities for this highly adaptable virus to establish sylvatic cycles that to date have not been seen outside of Africa. The short-term and long-term ecological consequences of such transmission cycles, including the impact on wildlife and people living in these areas, are completely unknown.

  18. Avian Influenza A Virus Infections in Humans

    Science.gov (United States)

    ... Past Newsletters Avian Influenza A Virus Infections in Humans Language: English Español Recommend on Facebook Tweet ... A Viruses Avian Influenza A Virus Infections in Humans Although avian influenza A viruses usually do not ...

  19. A New Definition of Virus

    Institute of Scientific and Technical Information of China (English)

    HE Hongjun; CAO Sihua; LUO Li; FENG Tao; PAN Li; ZOU Zhiji

    2006-01-01

    Security experts have not formally defined the distinction between viruses and normal programs. The paper takes user's intension as the criteria for malice, gives a formal definition of viruses that aim at stealing or destroying files, and proposes an algorithm to detect virus correctly. Compared with traditional definitions, this new definition is easy to understand, covers more malwares, adapts development of virus technology, and defines virus on the spot. The paper has also analyzed more than 250 real viruses and finds that they are all in the domain of the new definition, this implies that the new definition has great practical significance.

  20. [Zika virus infection during pregnancy].

    Science.gov (United States)

    Picone, O; Vauloup-Fellous, C; D'Ortenzio, E; Huissoud, C; Carles, G; Benachi, A; Faye, A; Luton, D; Paty, M-C; Ayoubi, J-M; Yazdanpanah, Y; Mandelbrot, L; Matheron, S

    2016-05-01

    A Zika virus epidemic is currently ongoing in the Americas. This virus is linked to congenital infections with potential severe neurodevelopmental dysfunction. However, incidence of fetal infection and whether this virus is responsible of other fetal complications are still unknown. National and international public health authorities recommend caution and several prevention measures. Declaration of Zika virus infection is now mandatory in France. Given the available knowledge on Zika virus, we suggest here a review of the current recommendations for management of pregnancy in case of suspicious or infection by Zika virus in a pregnant woman.

  1. Natural Gas Exploration in Sichuan Basin

    Institute of Scientific and Technical Information of China (English)

    Hu Guangcan

    1995-01-01

    @@ Sichuan Basin located in the eastern part of Sichuan province, is a structural basin with obvious boundaries . It is also a geographic basin and covers an area of about180×103 km2. The basin is surrounded by mountains with altitudes of 1000-3000m above sea level. The western part of the basin is the Chengdu Plain.The middle part of the basin is hills with altitudes of300-600m above sea level. The eastern part of the basin is featured by bar anticlinal hills with a northeast-southwest trend and are separated in the middle by open flatlands .The hills have an altitude of 700-800m above sea level.

  2. Geodynamics of the Sivas Basin (Turkey): from a forearc basin to a retroarc foreland basin

    Science.gov (United States)

    Legeay, Etienne; Ringenbach, Jean-Claude; Kergaravat, Charlie; Callot, Jean-Paul; Mohn, Geoffroy; Kavak, Kaan

    2016-04-01

    Anatolia records the consumption of several oceanic basins, from the Northern Neotethys domain, by north-dipping subduction until the end of Mesozoic. The associated obduction event occurred during Campanian, from North to South and from Greece to Oman, leading to the emplacement of ophiolite thrust sheets and associated ophiolitic mélange. In particular, the Sivas Basin in Eastern Anatolia is located at the boundary between the Kırsehir block to the East, Pontide arc to the North and Tauride Platform to the South, sutured by ophiolitic belts. The Sivas Basin formed a Tertiary fold-and-thrust belt, which exhibits mainly north verging thrust in Paleogene deposits, and South verging thrust in oligo-miocene sequence. To understand the northern verging thrust above south verging obduction, it is necessary to zoom out of the basin, and include a set of processes that affect the eastern Anatolia. This study aims to characterize the structural and sedimentary evolution of the Sivas Basin, based on a fieldwork approach, coupled to the interpretation of subsurface data, thermochronology and biostratigraphy. The Sivas Basin was initiated in a forearc setting relatively to the subduction of the Inner-Tauride Ocean while the associated ophiolites are obducted onto the northern passive margin of the Tauride margin. Early Maastrichtian to Paleocene deposits are represented by carbonate platforms located on ophiolitic highs, passing to turbidites and olistostomes toward the North. The early Eocene sediments, mainly composed of ophiolitic clasts, are deposited on a regional unconformity marked along the southern margin of the basin by incisions in response to the emergence of north-verging thrust. The middle Eocene sediments, intensively folded by northward thrusting, are mostly represented by flysch type deposits (olistostromes, mass-flows and turbidites). The onset of the compression is related to the initiation of the Taurus shortening in a retroarc situation, in response to

  3. Hepatitis viruses: Changing patterns of human disease

    OpenAIRE

    Purcell, R H

    1994-01-01

    Viral hepatitis is a disease of antiquity, but evidence for more than one etiologic agent has been recognized only since the 1940s, when two viruses (hepatitis A virus and hepatitis B virus) were thought to account for all disease. In the past 20 years, three additional hepatitis agents (hepatitis C virus, hepatitis D virus, and hepatitis E virus) have been discovered, and there is evidence for at least one additional virus. Each of the five recognized hepatitis viruses belongs to a different...

  4. Successor Characteristics of the Mesozoic and Cenozoic Songliao Basins

    Institute of Scientific and Technical Information of China (English)

    LI Zhongquan; Timothy KUSKY; YING Danlin; GUO Xiaoyu; LI Hongkui

    2008-01-01

    The Songliao basin is a complex successor basin that was initiated in the Mesozoic and experienced multiple periods of reactivation. Based on seismic and drilling data, as well as regional geologic research, we suggest that the Songliao basin contains several different successor basins resting on top of Carboniferous-Permian folded strata forming the basement to the Songliao basin. These basins include the Triassic-Mid Jurassic Paleo-foreland basin, the Late Jurassic-Early Cretaceous downfaulted basin, and an early Cretaceous depressed basin (since the Denglouku Group). This paper presents a systematic study of the basin-mountain interactions, and reveals that there are different types of prototype basin at different geologic times. These prototype basins sequentially superimposed and formed the large Songliao basin. Discovery of the Triassic-early Middle Jurassic paleo-foreland basin fills a Triassic-early Middle Jurassic gap in the geologic history of the Songliao basin. The paleoforeland basin, downfaulted basin, and depressed thermal subsidence basin all together represent the whole Mesozoic-Cenozoic geologic history and deformation of the Songliao basin. Discovery of the Triassic-early Middle Jurassic paleo-foreland basin plays an important role both for deep natural gas exploration and the study of basin-mountain coupling in north China and eastern China in general. This example gives dramatic evidence that we should give much more attention to the polyphase tectonic evolution of related basins for the next phase of exploration and study.

  5. THE ADVANCED CHEMISTRY BASINS PROJECT

    Energy Technology Data Exchange (ETDEWEB)

    William Goddard; Peter Meulbroek; Yongchun Tang; Lawrence Cathles III

    2004-04-05

    In the next decades, oil exploration by majors and independents will increasingly be in remote, inaccessible areas, or in areas where there has been extensive shallow exploration but deeper exploration potential may remain; areas where the collection of data is expensive, difficult, or even impossible, and where the most efficient use of existing data can drive the economics of the target. The ability to read hydrocarbon chemistry in terms of subsurface migration processes by relating it to the evolution of the basin and fluid migration is perhaps the single technological capability that could most improve our ability to explore effectively because it would allow us to use a vast store of existing or easily collected chemical data to determine the major migration pathways in a basin and to determine if there is deep exploration potential. To this end a the DOE funded a joint effort between California Institute of Technology, Cornell University, and GeoGroup Inc. to assemble a representative set of maturity and maturation kinetic models and develop an advanced basin model able to predict the chemistry of hydrocarbons in a basin from this input data. The four year project is now completed and has produced set of public domain maturity indicator and maturation kinetic data set, an oil chemistry and flash calculation tool operable under Excel, and a user friendly, graphically intuitive basin model that uses this data and flash tool, operates on a PC, and simulates hydrocarbon generation and migration and the chemical changes that can occur during migration (such as phase separation and gas washing). The DOE Advanced Chemistry Basin Model includes a number of new methods that represent advances over current technology. The model is built around the concept of handling arbitrarily detailed chemical composition of fluids in a robust finite-element 2-D grid. There are three themes on which the model focuses: chemical kinetic and equilibrium reaction parameters, chemical

  6. Molecular epidemiology of respiratory viruses in virus-induced asthma

    OpenAIRE

    HiroyukiTsukagoshi; TaiseiIshioka

    2013-01-01

    Acute respiratory illness (ARI) due to various viruses is not only the most common cause of upper respiratory infection in humans but is also a major cause of morbidity and mortality, leading to diseases such as bronchiolitis and pneumonia. Previous studies have shown that respiratory syncytial virus (RSV), human rhinovirus (HRV), human metapneumovirus (HMPV), human parainfluenza virus (HPIV), and human enterovirus (HEV) infections may be associated with virus-induced asthma. For example, it ...

  7. Ebola (Ebola Virus Disease): Transmission

    Science.gov (United States)

    ... Search The CDC Cancel Submit Search The CDC Ebola (Ebola Virus Disease) Note: Javascript is disabled or is ... message, please visit this page: About CDC.gov . Ebola (Ebola Virus Disease) About Ebola Questions & Answers 2014 ...

  8. Ebola (Ebola Virus Disease): Prevention

    Science.gov (United States)

    ... Search The CDC Cancel Submit Search The CDC Ebola (Ebola Virus Disease) Note: Javascript is disabled or is ... message, please visit this page: About CDC.gov . Ebola (Ebola Virus Disease) About Ebola Questions & Answers 2014 ...

  9. Ebola (Ebola Virus Disease): Treatment

    Science.gov (United States)

    ... Search The CDC Cancel Submit Search The CDC Ebola (Ebola Virus Disease) Note: Javascript is disabled or is ... message, please visit this page: About CDC.gov . Ebola (Ebola Virus Disease) About Ebola Questions & Answers 2014 ...

  10. Ebola (Ebola Virus Disease): Diagnosis

    Science.gov (United States)

    ... Search The CDC Cancel Submit Search The CDC Ebola (Ebola Virus Disease) Note: Javascript is disabled or is ... message, please visit this page: About CDC.gov . Ebola (Ebola Virus Disease) About Ebola Questions & Answers 2014 ...

  11. Zika Virus Infection and Microcephaly.

    Science.gov (United States)

    Millichap, J Gordon

    2016-01-01

    A Task Force established by the Brazil Ministry of Health investigated the possible association of microcephaly with Zika virus infection during pregnancy and a registry for microcephaly cases among women suspected to have had Zika virus infection during pregnancy.

  12. Epstein-Barr virus test

    Science.gov (United States)

    ... medlineplus.gov/ency/article/003513.htm Epstein-Barr virus antibody test To use the sharing features on this page, please enable JavaScript. Epstein-Barr virus antibody test is a blood test to detect ...

  13. Special Issue: Honey Bee Viruses

    Directory of Open Access Journals (Sweden)

    Sebastian Gisder

    2015-10-01

    Full Text Available Pollination of flowering plants is an important ecosystem service provided by wild insect pollinators and managed honey bees. Hence, losses and declines of pollinating insect species threaten human food security and are of major concern not only for apiculture or agriculture but for human society in general. Honey bee colony losses and bumblebee declines have attracted intensive research interest over the last decade and although the problem is far from being solved we now know that viruses are among the key players of many of these bee losses and bumblebee declines. With this special issue on bee viruses we, therefore, aimed to collect high quality original papers reflecting the current state of bee virus research. To this end, we focused on newly discovered viruses (Lake Sinai viruses, bee macula-like virus, or a so far neglected virus species (Apis mellifera filamentous virus, and cutting edge technologies (mass spectrometry, RNAi approach applied in the field.

  14. Salt Lake in Chaidamu Basin

    Institute of Scientific and Technical Information of China (English)

    王良华

    2007-01-01

    Chaidamu Basin(柴达木盆地) is in the west of China. It covers an area(地区) of 220,000 square kilometres(平方公里). The number of salt lakes(盐湖) is more than twenty in it. Chaerhan(察尔汗) Salt Lake is the largest in this area. If you get here, you will find that in the lake there is no water but a thick layer(层) of salt. You can walk in it without difficulty, and cars can come and go across it. The thickest layer of salt in this basin is about fifty metres thick. People tried their best to use the salt to build house...

  15. Response to the 2009-H1N1 influenza pandemic in the Mekong Basin: surveys of country health leaders

    OpenAIRE

    Dausey David J; Moore Melinda

    2011-01-01

    Abstract Background Soon after the 2009-H1N1 virus emerged as the first influenza pandemic in 41 years, countries had an early opportunity to test their preparedness plans, protocols and procedures, including their cooperation with other countries in responding to the global pandemic threat. The Mekong Basin Disease Surveillance cooperation (MBDS) comprises six countries - Cambodia, China (Yunnan and Guangxi Provinces), Lao People's Democratic Republic, Myanmar, Thailand and Vietnam - that fo...

  16. Avian influenza viruses in humans.

    OpenAIRE

    Malik Peiris, J S

    2009-01-01

    Past pandemics arose from low pathogenic avian influenza (LPAI) viruses. In more recent times, highly pathogenic avian influenza (HPAI) H5N1, LPAI H9N2 and both HPAI and LPAI H7 viruses have repeatedly caused zoonotic disease in humans. Such infections did not lead to sustained human-to-human transmission. Experimental infection of human volunteers and seroepidemiological studies suggest that avian influenza viruses of other subtypes may also infect humans. Viruses of the H7 subtype appear to...

  17. Basin stability in delayed dynamics

    Science.gov (United States)

    Leng, Siyang; Lin, Wei; Kurths, Jürgen

    2016-02-01

    Basin stability (BS) is a universal concept for complex systems studies, which focuses on the volume of the basin of attraction instead of the traditional linearization-based approach. It has a lot of applications in real-world systems especially in dynamical systems with a phenomenon of multi-stability, which is even more ubiquitous in delayed dynamics such as the firing neurons, the climatological processes, and the power grids. Due to the infinite dimensional property of the space for the initial values, how to properly define the basin’s volume for delayed dynamics remains a fundamental problem. We propose here a technique which projects the infinite dimensional initial state space to a finite-dimensional Euclidean space by expanding the initial function along with different orthogonal or nonorthogonal basis. A generalized concept of basin’s volume in delayed dynamics and a highly practicable calculating algorithm with a cross-validation procedure are provided to numerically estimate the basin of attraction in delayed dynamics. We show potential applicabilities of this approach by applying it to study several representative systems of biological or/and physical significance, including the delayed Hopfield neuronal model with multistability and delayed complex networks with synchronization dynamics.

  18. Hendra virus and Nipah virus animal vaccines.

    Science.gov (United States)

    Broder, Christopher C; Weir, Dawn L; Reid, Peter A

    2016-06-24

    Hendra virus (HeV) and Nipah virus (NiV) are zoonotic viruses that emerged in the mid to late 1990s causing disease outbreaks in livestock and people. HeV appeared in Queensland, Australia in 1994 causing a severe respiratory disease in horses along with a human case fatality. NiV emerged a few years later in Malaysia and Singapore in 1998-1999 causing a large outbreak of encephalitis with high mortality in people and also respiratory disease in pigs which served as amplifying hosts. The key pathological elements of HeV and NiV infection in several species of mammals, and also in people, are a severe systemic and often fatal neurologic and/or respiratory disease. In people, both HeV and NiV are also capable of causing relapsed encephalitis following recovery from an acute infection. The known reservoir hosts of HeV and NiV are several species of pteropid fruit bats. Spillovers of HeV into horses continue to occur in Australia and NiV has caused outbreaks in people in Bangladesh and India nearly annually since 2001, making HeV and NiV important transboundary biological threats. NiV in particular possesses several features that underscore its potential as a pandemic threat, including its ability to infect humans directly from natural reservoirs or indirectly from other susceptible animals, along with a capacity of limited human-to-human transmission. Several HeV and NiV animal challenge models have been developed which have facilitated an understanding of pathogenesis and allowed for the successful development of both active and passive immunization countermeasures. PMID:27154393

  19. Rhabdomyolysis Associated with Parainfluenza Virus

    Directory of Open Access Journals (Sweden)

    Miltiadis Douvoyiannis

    2013-01-01

    Full Text Available Influenza virus is the most frequently reported viral cause of rhabdomyolysis. A 7-year-old child is presented with rhabdomyolysis associated with parainfluenza type 2 virus. Nine cases of rhabdomyolysis associated with parainfluenza virus have been reported. Complications may include electrolyte disturbances, acute renal failure, and compartment syndrome.

  20. An introduction to computer viruses

    Energy Technology Data Exchange (ETDEWEB)

    Brown, D.R.

    1992-03-01

    This report on computer viruses is based upon a thesis written for the Master of Science degree in Computer Science from the University of Tennessee in December 1989 by David R. Brown. This thesis is entitled An Analysis of Computer Virus Construction, Proliferation, and Control and is available through the University of Tennessee Library. This paper contains an overview of the computer virus arena that can help the reader to evaluate the threat that computer viruses pose. The extent of this threat can only be determined by evaluating many different factors. These factors include the relative ease with which a computer virus can be written, the motivation involved in writing a computer virus, the damage and overhead incurred by infected systems, and the legal implications of computer viruses, among others. Based upon the research, the development of a computer virus seems to require more persistence than technical expertise. This is a frightening proclamation to the computing community. The education of computer professionals to the dangers that viruses pose to the welfare of the computing industry as a whole is stressed as a means of inhibiting the current proliferation of computer virus programs. Recommendations are made to assist computer users in preventing infection by computer viruses. These recommendations support solid general computer security practices as a means of combating computer viruses.

  1. Computer Bytes, Viruses and Vaccines.

    Science.gov (United States)

    Palmore, Teddy B.

    1989-01-01

    Presents a history of computer viruses, explains various types of viruses and how they affect software or computer operating systems, and describes examples of specific viruses. Available vaccines are explained, and precautions for protecting programs and disks are given. (nine references) (LRW)

  2. Protecting Your Computer from Viruses

    Science.gov (United States)

    Descy, Don E.

    2006-01-01

    A computer virus is defined as a software program capable of reproducing itself and usually capable of causing great harm to files or other programs on the same computer. The existence of computer viruses--or the necessity of avoiding viruses--is part of using a computer. With the advent of the Internet, the door was opened wide for these…

  3. Archaeal virus-host interactions

    NARCIS (Netherlands)

    Quax, T.E.F.

    2013-01-01

      The work presented in this thesis provides novel insights in several aspects of the molecular biology of archaea, bacteria and their viruses. Three fundamentally different groups of viruses are associated with the three domains of life. Archaeal viruses are characterized by a particularly

  4. Ipomoviruses: Squash vein yellowing virus, Cucumber vein yellowing virus, Cassava brown streak virus, and Ugandan cassava brown streak virus

    Science.gov (United States)

    Ipomoviruses including Squash vein yellowing virus, Cucumber vein yellowing virus and Cassava brown streak virus are currently causing significant economic impact on crop production in several regions of the world. Only recently have results of detailed characterization of their whitefly transmissi...

  5. Elucidating virus uptake and fusion by single virus tracing

    OpenAIRE

    Schupp, Dorothee Carolin

    2012-01-01

    Viruses are known to cause many diseases, from the common cold and cold sores to more serious diseases such as the Ebola virus disease and AIDS. Viruses have evolved different strategies to enter and infect cells. In order to infect a cell, viruses have to overcome the cell membrane barrier to deliver their genome to the site of replication. Enveloped viruses can either fuse directly at the plasma membrane or with an endosomal membrane after endocytic uptake. In this work, I studied the early...

  6. Relationship of lychnis ringspot virus to barley stripe mosaic virus and poa semilatent virus.

    Science.gov (United States)

    Hunter, B G; Smith, J; Fattouh, F; Jackson, A O

    1989-01-01

    Barley stripe mosaic virus (BSMV), poa semilatent virus (PSLV), and lychnis ringspot virus (LRSV) have previously been assigned to the hordeivirus group because of similarities in their particle morphology, physicochemical properties and serological analyses. However, the serological relationships of the three viruses have not been determined by direct comparison. The present study evaluated the relatedness of these viruses by Western and dot immunoblotting and by nucleic acid hybridizations. Serological analyses of the coat proteins separated by gel electrophoresis and of intact virus particles bound to nitrocellulose membranes revealed that BSMV and PSLV are distantly related, but that they are more closely related to each other than to LRSV. The genomic RNAs of the viruses failed to cross-hybridize in northern hybridization tests conducted at different temperatures. These comparisons showed that BSMV, PSLV and LRSV are distinct viruses with little nucleotide sequence relatedness. Thus our data provide additional support for their inclusion as separate members of the hordeivirus group. PMID:2722469

  7. Zika virus: Indian perspectives.

    Science.gov (United States)

    Mourya, Devendra T; Shil, Pratip; Sapkal, Gajanan N; Yadav, Pragya D

    2016-05-01

    The emergence of Zika virus (ZiV), a mosquito borne Flavivirus like dengue (DEN) and chikungunya (CHIK), in Brazil in 2014 and its spread to various countries have led to a global health emergency. Aedes aegypti is the major vector for ZiV. Fast dissemination of this virus in different geographical areas posses a major threat especially to regions where the population lacks herd immunity against the ZiV and there is abundance of Aedes mosquitoes. In this review, we focus on current global scenario, epidemiology, biology, diagnostic challenges and remedial measures for ZiVconsidering the Indian perspective. PMID:27487998

  8. Virus en Endodoncia

    OpenAIRE

    Hernández Vigueras, Scarlette; Salazar Navarrete, Luis; Pérez Tomás, Ricardo E.; Segura Egea, Juan José; Viñas, Miquel; López López, José

    2014-01-01

    La infección endodóntica es la infección que afecta al sistema de conductos radiculares y, sin duda, es el principal agente etiológico de las periodontitis apicales. Además, de las bacterias patógenas endodónticas, se ha buscado en los últimos años asociar la presencia de virus en distintos tipos de patología endodóntica. Los virus que más se han buscado y asociado son los pertenecientes a la familia herpesvirus, los cuales se han encontrado presentes en patologías periapicales principalmente...

  9. Tenosinovitis por virus Chikungunya

    Directory of Open Access Journals (Sweden)

    Alfredo Seijo

    2014-12-01

    Full Text Available Se presenta a la consulta un hombre proveniente de la República Dominicana con una tenosinovitis del extensor del dedo medio derecho; en la convalecencia inmediata, segunda curva febril luego de 48 horas de permanecer asintomático de una enfermedad febril aguda, y marcada astenia, exantema pruriginoso, poliartralgias con impotencia funcional y rigidez articular generalizada. Los exámenes bioquímicos no aportaron datos de interés para el diagnóstico. La serología para virus dengue fue negativa. La detección de IgM y de anticuerpos neutralizantes para virus Chikungunya (CHIKV fueron positivos.

  10. Zika virus: Indian perspectives

    Directory of Open Access Journals (Sweden)

    Devendra T Mourya

    2016-01-01

    Full Text Available The emergence of Zika virus (ZiV, a mosquito borne Flavivirus like dengue (DEN and chikungunya (CHIK, in Brazil in 2014 and its spread to various countries have led to a global health emergency. Aedes aegypti is the major vector for ZiV. Fast dissemination of this virus in different geographical areas posses a major threat especially to regions where the population lacks herd immunity against the ZiV and there is abundance of Aedes mosquitoes. In this review, we focus on current global scenario, epidemiology, biology, diagnostic challenges and remedial measures for ZiVconsidering the Indian perspective.

  11. Zika virus: Indian perspectives

    OpenAIRE

    Mourya, Devendra T.; Pratip Shil; Sapkal, Gajanan N.; Yadav, Pragya D

    2016-01-01

    The emergence of Zika virus (ZiV), a mosquito borne Flavivirus like dengue (DEN) and chikungunya (CHIK), in Brazil in 2014 and its spread to various countries have led to a global health emergency. Aedes aegypti is the major vector for ZiV. Fast dissemination of this virus in different geographical areas posses a major threat especially to regions where the population lacks herd immunity against the ZiV and there is abundance of Aedes mosquitoes. In this review, we focus on current global sce...

  12. Zika virus: Indian perspectives

    Science.gov (United States)

    Mourya, Devendra T.; Shil, Pratip; Sapkal, Gajanan N.; Yadav, Pragya D.

    2016-01-01

    The emergence of Zika virus (ZiV), a mosquito borne Flavivirus like dengue (DEN) and chikungunya (CHIK), in Brazil in 2014 and its spread to various countries have led to a global health emergency. Aedes aegypti is the major vector for ZiV. Fast dissemination of this virus in different geographical areas posses a major threat especially to regions where the population lacks herd immunity against the ZiV and there is abundance of Aedes mosquitoes. In this review, we focus on current global scenario, epidemiology, biology, diagnostic challenges and remedial measures for ZiVconsidering the Indian perspective. PMID:27487998

  13. [ZIKA--VIRUS INFECTION].

    Science.gov (United States)

    Velev, V

    2016-01-01

    This review summarizes the knowledge of the scientific community for Zika-virus infection. It became popular because of severe congenital damage causes of CNS in newborns whose mothers are infected during pregnancy, as well as the risk of pandemic distribution. Discusses the peculiarities of the biology and ecology of vectors--blood-sucking mosquitoes Aedes; stages in the spread of infection and practical problems which caused during pregnancy. Attention is paid to the recommendations that allow leading national and international medical organizations to deal with the threat Zika-virus infection.

  14. [ZIKA--VIRUS INFECTION].

    Science.gov (United States)

    Velev, V

    2016-01-01

    This review summarizes the knowledge of the scientific community for Zika-virus infection. It became popular because of severe congenital damage causes of CNS in newborns whose mothers are infected during pregnancy, as well as the risk of pandemic distribution. Discusses the peculiarities of the biology and ecology of vectors--blood-sucking mosquitoes Aedes; stages in the spread of infection and practical problems which caused during pregnancy. Attention is paid to the recommendations that allow leading national and international medical organizations to deal with the threat Zika-virus infection. PMID:27509655

  15. Bovine Virus Diarrhea (BVD)

    OpenAIRE

    Hoar, Bruce R.

    2004-01-01

    Bovine virus diarrhea (BVD) is a complicated disease to discuss as it can result in a wide variety of disease problems from very mild to very severe. BVD can be one of the most devastating diseases cattle encounter and one of the hardest to get rid of when it attacks a herd. The viruses that cause BVD have been grouped into two genotypes, Type I and Type II. The disease syndrome caused by the two genotypes is basically the same, however disease caused by Type II infection is often more severe...

  16. Sensing of RNA viruses

    DEFF Research Database (Denmark)

    Jensen, Søren; Thomsen, Allan Randrup

    2012-01-01

    Our knowledge regarding the contribution of the innate immune system in recognizing and subsequently initiating a host response to an invasion of RNA virus has been rapidly growing over the last decade. Descriptions of the receptors involved and the molecular mechanisms they employ to sense viral...... pathogen-associated molecular patterns have emerged in great detail. This review presents an overview of our current knowledge regarding the receptors used to detect RNA virus invasion, the molecular structures these receptors sense, and the involved downstream signaling pathways....

  17. Human Immunodeficiency Virus Prevention.

    Science.gov (United States)

    Davis, Teaniese Latham; DiClemente, Ralph

    2016-04-01

    Human immunodeficiency virus (HIV) is the virus that causes AIDS. Surveillance data from 2012 indicate an estimated 1.2 million people aged 13 years and older were living with HIV infection in the United States, and 12.8% do not know their status. There are approximately 50,000 new HIV infections annually. With no available cure for HIV, primary prevention to reduce incident cases of HIV is essential. Strategies to prevent HIV transmission include reducing sexual risk behavior and needle sharing. The Centers for Disease Control and Prevention has multiple resources available for primary and secondary prevention to reduce disease transmission and severity. PMID:26980130

  18. VHS virus - present situation

    DEFF Research Database (Denmark)

    Olesen, Niels Jørgen; Skall, Helle Frank

    themselves for VHS, several countries are still struggling with the disease. An update on the recent VHS outbreaks in rainbow trout in Iran, in olive flounder in Korea, in wrasse in Scotland, in turbot in Turkey, in a number of fish species in the great lakes in USA and Canada, and a general overview...... of the worldwide distribution of the disease will be given. Virus evolution: Recent studies indicate that only a few amino acid changes in the structural proteins of VHSV can change the virulence patterns significantly, thereby coming closer to assessing the risk of none to low virulent viruses becoming high...

  19. VHS virus - present situation

    DEFF Research Database (Denmark)

    Skall, Helle Frank; Olesen, Niels Jørgen

    2015-01-01

    themselves for VHS, several countries are still struggling with the disease. An update on the recent VHS outbreaks in rainbow trout in Iran, in olive flounder in Korea, in wrasse in Scotland, in turbot in Turkey, in a number of fish species in the great lakes in USA and Canada, and a general overview...... of the worldwide distribution of the disease will be given. Virus evolution: Recent studies indicate that only a few amino acid changes in the structural proteins of VHSV can change the virulence patterns significantly, thereby coming closer to assessing the risk of none to low virulent viruses becoming high...

  20. Research on computer virus database management system

    Science.gov (United States)

    Qi, Guoquan

    2011-12-01

    The growing proliferation of computer viruses becomes the lethal threat and research focus of the security of network information. While new virus is emerging, the number of viruses is growing, virus classification increasing complex. Virus naming because of agencies' capture time differences can not be unified. Although each agency has its own virus database, the communication between each other lacks, or virus information is incomplete, or a small number of sample information. This paper introduces the current construction status of the virus database at home and abroad, analyzes how to standardize and complete description of virus characteristics, and then gives the information integrity, storage security and manageable computer virus database design scheme.

  1. The geologic history of Margaritifer basin, Mars

    Science.gov (United States)

    Salvatore, M. R.; Kraft, M. D.; Edwards, Christopher; Christensen, P.R.

    2016-01-01

    In this study, we investigate the fluvial, sedimentary, and volcanic history of Margaritifer basin and the Uzboi-Ladon-Morava (ULM) outflow channel system. This network of valleys and basins spans more than 8000 km in length, linking the fluvially dissected southern highlands and Argyre Basin with the northern lowlands via Ares Vallis. Compositionally, thermophysically, and morphologically distinct geologic units are identified and are used to place critical relative stratigraphic constraints on the timing of geologic processes in Margaritifer basin. Our analyses show that fluvial activity was separated in time by significant episodes of geologic activity, including the widespread volcanic resurfacing of Margaritifer basin and the formation of chaos terrain. The most recent fluvial activity within Margaritifer basin appears to terminate at a region of chaos terrain, suggesting possible communication between surface and subsurface water reservoirs. We conclude with a discussion of the implications of these observations on our current knowledge of Martian hydrologic evolution in this important region.

  2. The geologic history of Margaritifer basin, Mars

    Science.gov (United States)

    Salvatore, M. R.; Kraft, M. D.; Edwards, C. S.; Christensen, P. R.

    2016-03-01

    In this study, we investigate the fluvial, sedimentary, and volcanic history of Margaritifer basin and the Uzboi-Ladon-Morava outflow channel system. This network of valleys and basins spans more than 8000 km in length, linking the fluvially dissected southern highlands and Argyre basin with the northern lowlands via Ares Vallis. Compositionally, thermophysically, and morphologically distinct geologic units are identified and are used to place critical relative stratigraphic constraints on the timing of geologic processes in Margaritifer basin. Our analyses show that fluvial activity was separated in time by significant episodes of geologic activity, including the widespread volcanic resurfacing of Margaritifer basin and the formation of chaos terrain. The most recent fluvial activity within Margaritifer basin appears to terminate at a region of chaos terrain, suggesting possible communication between surface and subsurface water reservoirs. We conclude with a discussion of the implications of these observations on our current knowledge of Martian hydrologic evolution in this important region.

  3. 75 FR 8895 - Basin Electric Power Cooperative: Deer Creek Station

    Science.gov (United States)

    2010-02-26

    ...; ] DEPARTMENT OF AGRICULTURE Rural Utilities Service Basin Electric Power Cooperative: Deer Creek Station AGENCY... potential environmental impacts of and alternatives to Basin Electric Power Cooperative's (Basin Electric... electric power in the eastern portion of Basin Electric's service area. Basin Electric's eastern...

  4. Drainage basin delineations for selected USGS streamflow-gaging stations in Virginia (Drainage_Basin)

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — The Drainage_Basin polygon feature class was created as a digital representation of drainage basins for more than 1,650 continuous-record streamflow-gaging...

  5. Viruses, Artificial Viruses and Virus-Based Structures for Biomedical Applications

    NARCIS (Netherlands)

    van Rijn, Patrick; Schirhagl, Romana

    2016-01-01

    Nanobiomaterials such as virus particles and artificial virus particles offer tremendous opportunities to develop new biomedical applications such as drug- or gene-delivery, imaging and sensing but also improve understanding of biological mechanisms. Recent advances within the field of virus-based s

  6. Reserves in western basins: Part 1, Greater Green River basin

    Energy Technology Data Exchange (ETDEWEB)

    1993-10-01

    This study characterizes an extremely large gas resource located in low permeability, overpressured sandstone reservoirs located below 8,000 feet drill depth in the Greater Green River basin, Wyoming. Total in place resource is estimated at 1,968 Tcf. Via application of geologic, engineering and economic criteria, the portion of this resource potentially recoverable as reserves is estimated. Those volumes estimated include probable, possible and potential categories and total 33 Tcf as a mean estimate of recoverable gas for all plays considered in the basin. Five plays (formations) were included in this study and each was separately analyzed in terms of its overpressured, tight gas resource, established productive characteristics and future reserves potential based on a constant $2/Mcf wellhead gas price scenario. A scheme has been developed to break the overall resource estimate down into components that can be considered as differing technical and economic challenges that must be overcome in order to exploit such resources: in other words, to convert those resources to economically recoverable reserves. Total recoverable reserves estimates of 33 Tcf do not include the existing production from overpressured tight reservoirs in the basin. These have estimated ultimate recovery of approximately 1.6 Tcf, or a per well average recovery of 2.3 Bcf. Due to the fact that considerable pay thicknesses can be present, wells can be economic despite limited drainage areas. It is typical for significant bypassed gas to be present at inter-well locations because drainage areas are commonly less than regulatory well spacing requirements.

  7. Reserve estimates in western basins. Part 2: Piceance Basin

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1995-10-01

    This study characterizes an extremely large gas resource located in low permeability, sandstone reservoirs of the Mesaverde group in the Piceance Basin, Colorado. Total in place resource is estimated at 307.3 Tcf. Via application of geologic, engineering and economic criteria, the portion of this resource potentially recoverable as reserves is estimated. Those volumes estimated include probable, possible and potential categories and total 5.8 Tcf as a mean estimate of recoverable gas for all plays considered in the basin. About 82.6% of the total evaluated resource is contained within sandstones that have extremely poor reservoir properties with permeabilities considered too low for commerciality using current frac technology. Cost reductions and technology improvements will be required to unlock portions of this enormous resource. Approximately 2.7% of the total resource is contained within sandstone reservoirs which do not respond to massive hydraulic fracture treatments, probably due to their natural lenticular nature. Approximately 6.8% of the total resource is located in deeply buried settings below deepest established production. Approximately 7.9% of the total resource is considered to represent tight reservoirs that may be commercially exploited using today`s hydraulic fracturing technology. Recent technology advances in hydraulic fracturing practices in the Piceance Basin Mesaverde has resulted in a marked improvement in per well gas recovery which, where demonstrated, has been incorporated into the estimates provided in this report. This improvement is so significant in changing the risk-reward relationship that has historically characterized this play, that previously uneconomic areas and resources will graduate to the economically exploitable category. 48 refs., 96 figs., 18 tabs.

  8. Pull-apart basin tectonic model is structurally impossible for Kashmir basin, NW Himalaya

    OpenAIRE

    Shah , A.A.

    2016-01-01

    Kashmir Basin in NW Himalaya is considered a Neogene-Quatermary piggyback basin that was formed as result of the continent-continent collision of Indian and Eurasian plates. This model however is recently challenged by a pull-apart basin model, which argues that a major dextral strike-slip fault through Kashmir basin is responsible for its formation. And here it is demonstrated that the new tectonic model is structurally problematic, and co...

  9. Hydrocarbon Migration and Accumulation in Dongying Basin

    Institute of Scientific and Technical Information of China (English)

    Li Chunguang; Shang Weirong

    1996-01-01

    @@ Dongying basin is a faulted basin rich in hydrocarbon and is under more exploration (wildcat well density is 0.155 well/km ) in the southern Bohai Bay area of China, covering an area of 5700 km. Based on the paleotopographic setting of Paleozoic basement rocks, the basin was evolved into Mesozoic and Cenozoic basin which was faulted in the north and overlapped in the south by the blockfaulting movement in J2-J3. So, each formation of the source rocks in Paleogene is characterized by thick deposit in the north and thin deposit in the south.

  10. Hack's law of debris-flow basins

    Institute of Scientific and Technical Information of China (English)

    LI Yong; YUE Z.Q.; LEE C.F.; BEIGHLEY R.E.; CHEN Xiao-Qing; HU Kai-Heng; CUI Peng

    2009-01-01

    Hack's law was originally derived from basin statistics for varied spatial scales and regions.The exponent value of the law has been shown to vary between 0.47 and 0.70,causing uncertainty in its application.This paper focuses on the emergence of Hack's law from debris-flow basins in China.Over 5,000 debris-flow basins in different regions of China with drainage areas less than 100km2 are included in this study.Basins in the different regions are found to present similar distributions.Hack's law is derived fi'om maximum probability and conditional distributions,suggesting that the law should describe some critical state of basin evolution.Results suggest the exponent value is approximately 0.5.Further analysis indicates that Hack's law is related to other scaling laws underlying the evolution of a basin and that the exponent is not dependent on basin shape but rather on the evolutionary stage.A case study of a well known debris-flow basin further confirms Hack's law and its implications in basin evolution.

  11. Basin Management under the Global Climate Change (Take North-East Asia Heilongjiang -Amur Basin and Taihu Basin For Example)

    Science.gov (United States)

    Liu, S.; Zhou, Z.; Zhong, G.; Zhang, X.

    2015-12-01

    The impact of global climate change on environment and society causes increasingly concern in different countries around the world. The main climate characteristic values, such as precipitation and temperature, have been changed, which leads to the variation of water resources, especially in large basins. Heilongjiang-Amur Basin and Taihu Basin are two large and important basins in China with large area and population. As global climate change and human activities have siganificant impacts on hydrology and water resources in two basins, the analysis of climate change are of great value. In this study, in Heilongjiang-Amur Basin, precipitation and temperature are investigated and their variation are predicted. And in Taihu Basin, precipitation including plum rain and typhoon, are studied and the variation trend of precipitation is predicted. Hence, the impacts of global climate change are assessed. From the result, it shows that the average temperature will continue to increase, and the precipitation will reduce first and then turn to increase in these two basins. It demonstrates that the water resources have been affected a lot by climate change as well as human activities. And these conclusions are provided as reference for policy makers and basin authorities in water resources management and natural hazards mitigation. Meanwhile, according to basins' particualr characters, the suggestions to future water resources management in two basins are given, and more scientific, comprehensive and sustained managements are required. Especially, in Heilongjiang-Amur River, which is a boundary river between China and Russia, it is very essential to enhance the cooperation between two countries.

  12. Relating petroleum system and play development to basin evolution: West African South Atlantic basins

    NARCIS (Netherlands)

    Beglinger, S.E.; Doust, H.; Cloetingh, S.A.P.L.

    2012-01-01

    Sedimentary basins can be classified according to their structural genesis and evolutionary history and the latter can be linked to petroleumsystem and playdevelopment. We propose an approach in which we use the established concepts in a new way: breaking basins down into their natural basin cycle d

  13. Fractal basin boundaries generated by basin cells and the geometry of mixing chaotic flows

    NARCIS (Netherlands)

    Nusse, HE; Yorke, JA

    2000-01-01

    Experiments and computations indicate that mixing in chaotic flows generates certain coherent spatial structures. Lf a two-dimensional basin has a basin cell (a trappings region whose boundary consists of pieces of the stable and unstable manifold of some periodic orbit) then the basin consists of a

  14. Viruses and febrile seizures

    NARCIS (Netherlands)

    Zeijl, J.H. van

    2004-01-01

    We conclude that viral infections are the main cause of febrile seizures, with an important role for influenza A, HHV-6 and HHV-7. We showed that several viral infections not only contribute to initial febrile seizures, but also to recurrences. Viruses could not be detected in the CSF of children wi

  15. The virus of management

    DEFF Research Database (Denmark)

    Kjær, Peter; Frankel, Christian

    2003-01-01

    of concrete management knowledge and practice. The article isstructured as follows. After the introduction, we first develop the notion of organizational virus asinto an analytical approach. Second, we discern in the work of Frederick Taylor on scientificmanagement and Max Weber on bureaucracy, two quite...

  16. Viruses of Haloarchaea

    Directory of Open Access Journals (Sweden)

    Alison W. S. Luk

    2014-11-01

    Full Text Available In hypersaline environments, haloarchaea (halophilic members of the Archaea are the dominant organisms, and the viruses that infect them, haloarchaeoviruses are at least ten times more abundant. Since their discovery in 1974, described haloarchaeoviruses include head-tailed, pleomorphic, spherical and spindle-shaped morphologies, representing Myoviridae, Siphoviridae, Podoviridae, Pleolipoviridae, Sphaerolipoviridae and Fuselloviridae families. This review overviews current knowledge of haloarchaeoviruses, providing information about classification, morphotypes, macromolecules, life cycles, genetic manipulation and gene regulation, and host-virus responses. In so doing, the review incorporates knowledge from laboratory studies of isolated viruses, field-based studies of environmental samples, and both genomic and metagenomic analyses of haloarchaeoviruses. What emerges is that some haloarchaeoviruses possess unique morphological and life cycle properties, while others share features with other viruses (e.g., bacteriophages. Their interactions with hosts influence community structure and evolution of populations that exist in hypersaline environments as diverse as seawater evaporation ponds, to hot desert or Antarctic lakes. The discoveries of their wide-ranging and important roles in the ecology and evolution of hypersaline communities serves as a strong motivator for future investigations of both laboratory-model and environmental systems.

  17. Virus spread in networks

    NARCIS (Netherlands)

    Mieghem, P. van; Omic, J.; Kooij, R.E.

    2009-01-01

    The influence of the network characteristics on the virus spread is analyzed in a new-the N-intertwined Markov chain-model, whose only approximation lies in the application of mean field theory. The mean field approximation is quantified in detail. The N-intertwined model has been compared with the

  18. The Virus Business

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    The Panda, a computer infection that wreaked havoc on computers across China, brought attention to the country’s underground computer virus business, but it was just the tip of a growing iceberg The cute and cuddly panda, a nation- al treasure of China

  19. Viruses of haloarchaea.

    Science.gov (United States)

    Luk, Alison W S; Williams, Timothy J; Erdmann, Susanne; Papke, R Thane; Cavicchioli, Ricardo

    2014-01-01

    In hypersaline environments, haloarchaea (halophilic members of the Archaea) are the dominant organisms, and the viruses that infect them, haloarchaeoviruses are at least ten times more abundant. Since their discovery in 1974, described haloarchaeoviruses include head-tailed, pleomorphic, spherical and spindle-shaped morphologies, representing Myoviridae, Siphoviridae, Podoviridae, Pleolipoviridae, Sphaerolipoviridae and Fuselloviridae families. This review overviews current knowledge of haloarchaeoviruses, providing information about classification, morphotypes, macromolecules, life cycles, genetic manipulation and gene regulation, and host-virus responses. In so doing, the review incorporates knowledge from laboratory studies of isolated viruses, field-based studies of environmental samples, and both genomic and metagenomic analyses of haloarchaeoviruses. What emerges is that some haloarchaeoviruses possess unique morphological and life cycle properties, while others share features with other viruses (e.g., bacteriophages). Their interactions with hosts influence community structure and evolution of populations that exist in hypersaline environments as diverse as seawater evaporation ponds, to hot desert or Antarctic lakes. The discoveries of their wide-ranging and important roles in the ecology and evolution of hypersaline communities serves as a strong motivator for future investigations of both laboratory-model and environmental systems. PMID:25402735

  20. Bat flight and zoonotic viruses

    Science.gov (United States)

    O'Shea, Thomas; Cryan, Paul M.; Cunningham, Andrew A.; Fooks, Anthony R.; Hayman, David T.S.; Luis, Angela D.; Peel, Alison J.; Plowright, Raina K.; Wood, James L.N.

    2014-01-01

    Bats are sources of high viral diversity and high-profile zoonotic viruses worldwide. Although apparently not pathogenic in their reservoir hosts, some viruses from bats severely affect other mammals, including humans. Examples include severe acute respiratory syndrome coronaviruses, Ebola and Marburg viruses, and Nipah and Hendra viruses. Factors underlying high viral diversity in bats are the subject of speculation. We hypothesize that flight, a factor common to all bats but to no other mammals, provides an intensive selective force for coexistence with viral parasites through a daily cycle that elevates metabolism and body temperature analogous to the febrile response in other mammals. On an evolutionary scale, this host–virus interaction might have resulted in the large diversity of zoonotic viruses in bats, possibly through bat viruses adapting to be more tolerant of the fever response and less virulent to their natural hosts.

  1. Suceava Anthropic Torrential Basin - Prolegomena

    Directory of Open Access Journals (Sweden)

    Andrei-Emil BRICIU

    2010-05-01

    Full Text Available One problem discussed by urban hydrology today is the draining influence of the modern cities over the natural drainage systems. The increasing urban areas and of their imperviousness all over theworld is linked to floods shape modifications and unpredicted systemic implications.  Generally, the draining influence of a city over its environment begins when it has a surface great enough to create an anthropic-generated runoff during a rain with enoughprecipitations to provoke waters accumulation into street torrents. The size, imperviousness, precipitations, drainage system and water consumption of the Suceava city are analysed in order to estimate the discharge of the city into Suceava river at various rainfalls. The article is structured as follows:1. Argumentation on the class separation between natural and anthropic torrential basins.2. Placing Suceava city as one of the torrential anthropic basins in Romania using basic arguments.3. Extending one of the argument, the importance of the rainfalls, in more detailed discussions (rainfall characteristics mainly, but also its cumulative effect with the floods on the Suceava river and the consumption of water in the city, with two scenarios. 4. The city is analysed as being integrated into a metropolitan area which can exacerbate the influence of the main city over the surrounding natural drainage basins nearby that area.5. Conclusions, where measures are proposed in order to diminish the potential negative effects on environment and human society.This article is only an introduction to a more detailed analysis which will be complete with further field data.

  2. Molecular epidemiology of respiratory viruses in virus-induced asthma

    Directory of Open Access Journals (Sweden)

    Hiroyuki eTsukagoshi

    2013-09-01

    Full Text Available Acute respiratory illness (ARI due to various viruses is not only the most common cause of upper respiratory infection in humans but is also a major cause of morbidity and mortality, leading to diseases such as bronchiolitis and pneumonia. Previous studies have shown that respiratory syncytial virus (RSV, human rhinovirus (HRV, human metapneumovirus (HMPV, human parainfluenza virus (HPIV, and human enterovirus (HEV infections may be associated with virus-induced asthma. For example, it has been suggested that HRV infection is detected in the acute exacerbation of asthma and infection is prolonged. Thus it is believed that the main etiological cause of asthma is ARI viruses. Furthermore, the number of asthma patients in most industrial countries has greatly increased, resulting in a morbidity rate of around 10-15% of the population. However, the relationships between viral infections, host immune response, and host factors in the pathophysiology of asthma remain unclear. To gain a better understanding of the epidemiology of virus-induced asthma, it is important to assess both the characteristics of the viruses and the host defense mechanisms. Molecular epidemiology enables us to understand the pathogenesis of microorganisms by identifying specific pathways, molecules, and genes that influence the risk of developing a disease. However, the epidemiology of various respiratory viruses associated with virus-induced asthma is not fully understood. Therefore, in this article, we review molecular epidemiological studies of RSV, HRV, HPIV, and HMPV infection associated with virus-induced asthma.

  3. Seismic Characterization of the Jakarta Basin

    Science.gov (United States)

    Cipta, A.; Saygin, E.; Cummins, P. R.; Masturyono, M.; Rudyanto, A.; Irsyam, M.

    2015-12-01

    Jakarta, Indonesia, is home to more than 10 million people. Many of these people live in seismically non-resilient structures in an area that historical records suggest is prone to earthquake shaking. The city lies in a sedimentary basin composed of Quaternary alluvium that experiences rapid subsidence (26 cm/year) due to groundwater extraction. Forecasts of how much subsidence may occur in the future are dependent on the thickness of the basin. However, basin geometry and sediment thickness are poorly known. In term of seismic hazard, thick loose sediment can lead to high amplification of seismic waves, of the kind that led to widespread damage in Mexico city during the Michoacan Earthquake of 1985. In order to characterize basin structure, a temporary seismograph deployment was undertaken in Jakarta in Oct 2013- Jan 2014. A total of 96 seismic instrument were deployed throughout Jakarta were deployed throughout Jakarta at 3-5 km spacing. Ambient noise tomography was applied to obtain models of the subsurface velocity structure. Important key, low velocity anomalies at short period (<8s) correspond to the main sedimentary sub-basins thought to be present based on geological interpretations of shallow stratigraphy in the Jakarta Basin. The result shows that at a depth of 300 m, shear-wave velocity in the northern part (600 m/s) of the basin is lower than that in the southern part. The most prominent low velocity structure appears in the northwest of the basin, down to a depth of 800 m, with velocity as low as 1200 m/s. This very low velocity indicates the thickness of sediment and the variability of basin geometry. Waveform computation using SPECFEM2D shows that amplification due to basin geometry occurs at the basin edge and the thick sediment leads to amplification at the basin center. Computation also shows the longer shaking duration occurrs at the basin edge and center of the basin. The nest step will be validating the basin model using earthquake events

  4. Presence and Distribution of Tobacco Viruses in Serbia

    OpenAIRE

    Nataša Duduk; Aleksandra Bulajić; Janoš Berenji; Ivana Đekić; Bojan Duduk; Branka Krstić

    2006-01-01

    Infection with a large number of plant viruses could imperil tobacco yield and quality. Tobacco is a natural host for more than 20 viruses, among which the most important and economically harmful are tobacco mosaic virus (TMV), tomato spotted wilt virus (TSWV), cucumber mosaic virus (CMV), potato virus Y (PVY), alfalfa mosaic virus (AMV), tobacco ringspot virus (TRSV), tobacco each virus (TEV) and tobacco vein mottling virus (TVMV).The occurence and distribution of tobacco viruses were invest...

  5. Neuroteratogenic Viruses and Lessons for Zika Virus Models.

    Science.gov (United States)

    Kim, Kenneth; Shresta, Sujan

    2016-08-01

    The Centers for Disease Control and Prevention has confirmed that Zika virus (ZIKV) causes congenital microcephaly. ZIKV now joins five other neuroteratogenic (NT) viruses in humans and ZIKV research is in its infancy. In addition, there is only one other NT human arbovirus (Venezuelan equine encephalitis virus), which is also poorly understood. But further insight into ZIKV can be found by evaluating arboviruses in domestic animals, of which there are at least seven NT viruses, three of which have been well studied. Here we review two key anatomical structures involved in modeling transplacental NT virus transmission: the placenta and the fetal blood-brain barrier. We then survey major research findings regarding transmission of NT viruses for guidance in establishing a mouse model of Zika disease that is crucial for a better understanding of ZIKV transmission and pathogenesis. PMID:27387029

  6. HYDROGEOLOGICAL INVESTIGATIONS OF SANDIKLI BASIN (AFYONKARAHISAR)-PRELIMINARY STUDIES

    OpenAIRE

    Seyman Aksever, Arş. Gör. Fatma; Davraz, Doç.Dr.Ayşen; Karagüzel, Prof.Dr.Remzi

    2011-01-01

    ABSTRACT The aims of this research are to determine hydrogeological and hydrogeochemical investigations of Sandıklı (Afyonkarahisar) basin and to plan of optimum groundwater management of the basin. The Sandıklı basin is situated in the west of the Aegean region and discharge to the Büyük Menderes Basin. The research area is occurred from Sandıklı Kuruçay basin and Küçük Sincanlı basin. It has 1556 km2 recharge area. The basin has a semi-closed basin property due to discharge to Kestel Ri...

  7. Red River Basin Mapping 2008-2010

    Data.gov (United States)

    Federal Emergency Management Agency, Department of Homeland Security — This LiDAR data covers the Red River Basin with portions of ND, MN, SD and flows into Canada. The US Red River Basin boudnary covers 40,860 sqmi,with the additional...

  8. Fractal basins in an ecological model

    Directory of Open Access Journals (Sweden)

    I. Djellit

    2013-09-01

    Full Text Available Complex dynamics is detected in an ecological model of host-parasitoid interaction. It illustrates fractalization of basins with self-similarity and chaotic attractors. This paper describes these dynamic behaviors, bifurcations, and chaos. Fractals basins are displayed by numerical simulations.

  9. Hydrological research basins and the environment

    NARCIS (Netherlands)

    1990-01-01

    The International Conference on Hydrological Research Basins and the Environment, was held at Wageningen from September 24th to 28th. The conference was organized by the Committee of the European Network of Experimental and Representative Basins and the National Committee of the Netherlands for the

  10. The structure of Nansen and Amundsen Basin

    Science.gov (United States)

    Micksch, U.; Jokat, W.

    2003-04-01

    During the AMORE expedition in August/September 2001, a US-German joint project, the Gakkel Ridge and the adjoining basins were investigated. In this contribution we report on the results of the seismic investigations in the Nansen Basin as well as in the Amundsen Basin. We obtained two almost parallel profiles through the Nansen Basin from the northeastern continental margin of Svalbard (29°E and 32°E) to the Gakkel Ridge at 17°E and 21°E. To investigate the Amundsen Basin, we left Gakkel Ridge at about 70°E towards Lomonossov Ridge for another transect. In total 1360 km of seismic reflection data with very good data quality were recorded. Parallel to the seismic reflection transects up to 30 sonobuoys were deployed. The data from both basins shows striking differences in the basement topography. While in the Nansen Basin the oceanic crust is more or less continuously shallowing, this is not the case in the Amundsen Basin. This points to some asymmetric spreading history of the Gakkel Ridge in northern and southern directions. Some of the Sonobuoys recorded also weak arrivals from the Moho discontinuity. The analysis of the wide angle and gravity data shows, that there are areas with a significant thinning of the oceanic crust. Results of the reflection and refraktion seismic as well as the gravity interpretation will be presented.

  11. Deep controls on intraplate basin inversion

    DEFF Research Database (Denmark)

    Nielsen, S.B.; Stephenson, Randell Alexander; Schiffer, Christian

    2014-01-01

    Basin inversion is an intermediate-scale manifestation of continental intraplate deformation, which produces earthquake activity in the interior of continents. The sedimentary basins of central Europe, inverted in the Late Cretaceous– Paleocene, represent a classic example of this phenomenon. It ...

  12. K-Basin isolation barrier seal

    International Nuclear Information System (INIS)

    This report documents various aspects of the design, analysis, procurement, and fabrication of the hydraulic seal on the isolation barriers to be installed in the 100-K Area spent nuclear fuel basin. The isolation barrier is used to keep water in the basin in the event of an earthquake

  13. Basin Fluid Mineralization during Multistage Evolution of the Lanping Sedimentary Basin, Southwestern China

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    The Lanping sedimentary basin has experienced a five-stage evolution since the late Paleozoic: ocean-continent transformation (late Paleozoic to early mid-Triassic); intracontinental rift basin (late mid-Triassic to early Jurassic); down-warped basin (middle to late Jurassic); foreland basin (Cretaceous); and strike-slip basin (Cenozoic). Three major genetic types of Ag-Cu polymetailic ore deposits, including the reworked hydrothermal sedimentary, sedimentary-hydrothermally reworked and hydrothermal vein types, are considered to be the products of basin fluid activity at specific sedimentary-tectonic evolutionary stages. Tectonic differences of the different evolutionary stages resulted in considerable discrepancy in the mechanisms of formation-transportation, migration direction and emplacement processes of the basin fluids, thus causing differences in mineralization styles as well as in genetic types of ore deposit.

  14. Rapid Detection and Quantification of RNA of Ebola and Marburg Viruses, Lassa Virus, Crimean-Congo Hemorrhagic Fever Virus, Rift Valley Fever Virus, Dengue Virus, and Yellow Fever Virus by Real-Time Reverse Transcription-PCR

    OpenAIRE

    Drosten, Christian; Göttig, Stephan; Schilling, Stefan; Asper, Marcel; Panning, Marcus; Schmitz, Herbert; Günther, Stephan

    2002-01-01

    Viral hemorrhagic fevers (VHFs) are acute infections with high case fatality rates. Important VHF agents are Ebola and Marburg viruses (MBGV/EBOV), Lassa virus (LASV), Crimean-Congo hemorrhagic fever virus (CCHFV), Rift Valley fever virus (RVFV), dengue virus (DENV), and yellow fever virus (YFV). VHFs are clinically difficult to diagnose and to distinguish; a rapid and reliable laboratory diagnosis is required in suspected cases. We have established six one-step, real-time reverse transcripti...

  15. Characterization of a synergistic interaction between two cucurbit-infecting begomoviruses: Squash leaf curl virus and Watermelon chlorotic stunt virus.

    Science.gov (United States)

    Sufrin-Ringwald, Tali; Lapidot, Moshe

    2011-02-01

    Squash leaf curl virus (SLCV) and Watermelon chlorotic stunt virus (WmCSV) are cucurbit-infecting bipartite begomoviruses. Both viruses are found in the eastern Mediterranean basin but the effects of dual infection of both viruses on melon (Cucumis melo L.) have not been described. 'Arava' melon plants were inoculated in the greenhouse, using whiteflies, with either SLCV, WmCSV, or both. Control plants were exposed to nonviruliferous whiteflies or not exposed at all. Following inoculation, plants were transplanted to a 50-mesh insect-proof nethouse and grown until fruit maturity. The experiment was performed in two melon-growing seasons: spring, transplant in May and harvest in July; and summer, transplant in August and harvest in October. Following inoculation, SLCV-infected melon plants showed mild symptoms that disappeared with time, and there was no effect on plant height. WmCSV-infected plants developed disease symptoms that became more obvious with time, and plants were somewhat shorter than control plants in the spring but not in the summer. SLCV had no effect on yield, regardless of season. WmCSV had no statistically significant effect on yield in the spring but, in the summer, reduced yield by 22%, on average. Dual-inoculated plants showed a synergistic interaction between the two viruses. They developed disease symptoms that were more pronounced than WmCSV alone, with plants being shorter than control plants by 20 to 25% regardless of season. Moreover, the yield of dual-inoculated plants was reduced on average by 21% in the spring and 54% in the summer, and fruit appearance was adversely affected. Dual inoculation did not affect WmCSV DNA level but SLCV DNA level was increased several-fold by the presence of WmCSV.

  16. Neotectonic of subsiding basins : case of studies from Marañon and Beni basins, Peru and Bolivia

    OpenAIRE

    Dumont, Jean-Francois

    1994-01-01

    Climatic conditions make the fluvial processes very sensitive in the extended flood plain of subandean basins, giving typical morphostructures. Because of high subsidence rate, these basins are case for the understanding of neotectonics in subsiding basins. Recent anciente fluvial traces are used in combination with sub surface structures, neotectonic and seismotectonic data to study the neotectonic evolution of the Peruvian and Bolivian active foreland basins. These basins, the Marañon Basin...

  17. Implementing Integrated River Basin Management in China

    Directory of Open Access Journals (Sweden)

    Lifeng Li

    2010-06-01

    Full Text Available This paper examines the role of the World Wildlife Fund for Nature China as policy entrepreneur in China. It illustrates the ways in which the World Wildlife Fund for Nature is active in promoting integrated river basin management in the Yangtze River basin and how the efforts at basin level are matched with the advice of the China Council for International Cooperation on Environment and Development task force on integrated river basin management to the national government of China. This article demonstrates that the World Wildlife Fund for Nature uses various strategies of different types to support a transition process towards integrated river basin management. Successful deployment of these strategies for change in environmental policy requires special skills, actions, and attitudes on the part of the policy entrepreneur, especially in China, where the government has a dominant role regarding water management and the position of policy entrepeneurs is delicate.

  18. Determination of the Relationship between Hydrologic Processes and Basin Morphometry - The Lamos Basin (Mersin, Turkey)

    Science.gov (United States)

    Yıldırım, Ümit; Güler, Cüneyt

    2016-04-01

    This study has been carried out to determine the relationship between hydrologic processes and basin morphometry in the Lamos Basin, which is located at the northern part of the Mersin (SE Turkey). The morphometric parameters of the basin was derived from the 1:25K scale topographic map sheets that were digitized using ArcGIS 9.3.1 geographic information system (GIS) software. Morphometric parameters considered in this study include basin area, basin length, basin perimeter length, stream order, stream number, stream length, mean stream length, basin relief, drainage density, stream frequency, drainage texture, bifurcation ratio, form factor, elongation ratio, overland flow length, relief ratio, and hypsometric integral. The results have shown that there are 1252 individual stream reaches with a total length of 1414.1 km in the Lamos basin, which covers an area of 1358 km2 and has a length of 103 km in the N-S direction. Furthermore, the basin has a medium drainage density of 1.04 1/km with a stream frequency and drainage texture values of 0.92 and 4.33, respectively. The basin can be classified as elongated because of the low values of elongation ratio (0.48) and form factor (0.12). The hypsometric integral of the basin (0.58) indicates that it is in the youth period and thus reasonably sensitive to erosion. The values of drainage texture, drainage density, and stream frequency indicate that the Lamos basin is moderately well drained, therefore overland flow in the basin is not expected to be so quick. Thus, in case of occurrence of sudden peak flows, sensitivity to the land sliding and erosion may increase further. As a result, it is suggested that human activities in the basin should be limited in areas in fairly close proximity to the present day stream network to prevent or reduce the risk to life and property.

  19. Vade retro virus

    OpenAIRE

    Bardini, Thierry

    2015-01-01

    Avec l’aide de récents résultats de la virologie, le présent article se concentre sur les virus en tant que participants d’une possible redéfinition de la frontière inférieure de la vie, en tant que vie minimale. À l’heure du triomphe de la viralité dans la cyberculture contemporaine, l’auteur avance que l’on devrait considérer les virus comme les premières formes d’entités convergentes, c’est-à-dire existant par-delà la division du monde en deux phases distinctes et incompatibles, numérique ...

  20. [Ebola virus disease: Update].

    Science.gov (United States)

    de la Calle-Prieto, Fernando; Arsuaga-Vicente, Marta; Mora-Rillo, Marta; Arnalich-Fernandez, Francisco; Arribas, Jose Ramon

    2016-01-01

    The first known Ebola outbreak occurred in 1976. Since then, 24 limited outbreaks had been reported in Central Africa, but never affecting more than 425 persons. The current outbreak in Western Africa is the largest in history with 28,220 reported cases and 11,291 deaths. The magnitude of the epidemic has caused worldwide alarm. For the first time, evacuated patients were treated outside Africa, and secondary cases have occurred in Spain and the United States. Since the start of the current epidemic, our knowledge about the epidemiology, clinical picture, laboratory findings, and virology of Ebola virus disease has considerably expanded. For the first time, experimental treatment has been tried, and there have been spectacular advances in vaccine development. A review is presented of these advances in the knowledge of Ebola virus disease. PMID:26774254

  1. Ebola, the killer virus

    OpenAIRE

    Ghazanfar, Haider; Orooj, Fizza; Abdullah, Muhammad Ahmed; Ghazanfar, Ali

    2015-01-01

    Ebola virus disease (EVD) has mostly affected economically deprived countries as limited resources adversely affect a country’s infrastructure and administration. Probing into the factors that led to the widespread outbreak, setting forth plans to counter EVD cases in developing countries, and devising definitive measures to limit the spread of the disease are essential steps that must be immediately taken. In this review we summarize the pathogenesis of EVD and the factors that led to its sp...

  2. Ebola Virus Disease

    Centers for Disease Control (CDC) Podcasts

    2014-08-08

    This podcast provides general information about Ebola virus disease and the outbreak in West Africa. The program contains remarks from CDC Director Dr. Tom Frieden, as well as a brief description of CDC’s response efforts.  Created: 8/8/2014 by National Center for Emerging and Zoonotic Infectious Diseases (NCEZID).   Date Released: 8/8/2014.

  3. Viruses and drinking water

    OpenAIRE

    Cartwright, R Y

    1997-01-01

    There is no evidence to indicate that there is a risk of acquiring a virus infection through the consumption of properly treated drinking water, provided the integrity of the distribution system is maintained and there is no post-treatment contamination. The consumption of inadequately treated, untreated or post-treatment contaminated water is, however, associated with a risk of hepatitis A, hepatitis E and viral gastroenteritis. The use of the standard bacterial indicators for water monitori...

  4. Geographic distribution of Bhanja virus.

    Science.gov (United States)

    Hubálek, Z

    1987-01-01

    A review on the geographic distribution, vectors and hosts of Bhanja virus (Bunyaviridae) is based on reports about: isolations of the virus; antibody surveys. Bhanja virus has been isolated in 15 countries of Asia, Africa and Europe, and antibodies against it have been detected in 15 additional countries. Vector range includes ticks of the family Ixodidae (subfam. Amblyomminae; not subfam. Ixodinae): 13 species of 6 genera (Haemaphysalis, Dermacentor, Hyalomma, Amblyomma, Rhipicephalus and Boophilus) yielded the virus. Bhanja virus has only rarely been isolated from vertebrates (Atelerix, Xerus, Ovis, Bos; possibly bats), though antibodies have been detected frequently in a wide range of mammals (Ruminantia being the major hosts), in several species of birds (Passeriformes, Galliformes) and even reptiles (Ophisaurus apodus). Natural foci of the Bhanja virus infections are of the boskematic type (sensu Rosický), associated closely with pastures of domestic ruminants infested by ticks in the regions of tropical, subtropical and partly temperate climatic zones. PMID:3108117

  5. Parainfluenza Virus Infection.

    Science.gov (United States)

    Branche, Angela R; Falsey, Ann R

    2016-08-01

    Human parainfluenza viruses (HPIVs) are single-stranded, enveloped RNA viruses of the Paramyoviridaie family. There are four serotypes which cause respiratory illnesses in children and adults. HPIVs bind and replicate in the ciliated epithelial cells of the upper and lower respiratory tract and the extent of the infection correlates with the location involved. Seasonal HPIV epidemics result in a significant burden of disease in children and account for 40% of pediatric hospitalizations for lower respiratory tract illnesses (LRTIs) and 75% of croup cases. Parainfluenza viruses are associated with a wide spectrum of illnesses which include otitis media, pharyngitis, conjunctivitis, croup, tracheobronchitis, and pneumonia. Uncommon respiratory manifestations include apnea, bradycardia, parotitis, and respiratory distress syndrome and rarely disseminated infection. Immunity resulting from disease in childhood is incomplete and reinfection with HPIV accounts for 15% of respiratory illnesses in adults. Severe disease and fatal pneumonia may occur in elderly and immunocompromised adults. HPIV pneumonia in recipients of hematopoietic stem cell transplant (HSCT) is associated with 50% acute mortality and 75% mortality at 6 months. Though sensitive molecular diagnostics are available to rapidly diagnose HPIV infection, effective antiviral therapies are not available. Currently, treatment for HPIV infection is supportive with the exception of croup where the use of corticosteroids has been found to be beneficial. Several novel drugs including DAS181 appear promising in efforts to treat severe disease in immunocompromised patients, and vaccines to decrease the burden of disease in young children are in development. PMID:27486735

  6. Lagos Bat Virus in Kenya▿

    OpenAIRE

    Kuzmin, Ivan V.; Niezgoda, Michael; Franka, Richard; Agwanda, Bernard; Markotter, Wanda; Beagley, Janet C.; Urazova, Olga Y.; Breiman, Robert F.; Rupprecht, Charles E.

    2008-01-01

    During lyssavirus surveillance, 1,221 bats of at least 30 species were collected from 25 locations in Kenya. One isolate of Lagos bat virus (LBV) was obtained from a dead Eidolon helvum fruit bat. The virus was most similar phylogenetically to LBV isolates from Senegal (1985) and from France (imported from Togo or Egypt; 1999), sharing with these viruses 100% nucleoprotein identity and 99.8 to 100% glycoprotein identity. This genome conservancy across space and time suggests that LBV is well ...

  7. Photoreactivation of a Cytoplasmic Virus

    Science.gov (United States)

    Pfefferkorn, E. R.; Boyle, Mary K.

    1972-01-01

    Ultraviolet light-inactivated frog virus 3 is efficiently photoreactivated by chick embryo cells. A cellular enzyme is presumably responsible for this repair of viral deoxyribonucleic acid, for the phenomenon is insensitive to an inhibitor of protein synthesis and is not seen in mammalian cells that are known to lack photoreactivating enzyme. Since frog virus 3 is a cytoplasmic virus, functionally significant amounts of photoreactivating enzyme are probably present in the cytoplasm of chick embryo cells. PMID:5062749

  8. Delaware basin/Central basin platform margin: The development of a subthrust deep-gas province in the Permian Basin

    Energy Technology Data Exchange (ETDEWEB)

    Purves, W.J. (Mobil Oil Corp., Midland, TX (USA)); Ting, S.C. (Mobil, Farmers Branch, TX (USA))

    1990-05-01

    A deep-gas-prone province was identified along the Delaware basin/Central Basin platform margin, a margin conventionally interpreted to be bounded by high-angle normal or high-angle reverse structures. Redefinition of the tectonic style between the Delaware basin and the adjacent platform resulted in the identification of this Delaware basin/Central Basin platform subthrust province and a giant prospect within it. Definition of a giant-sized gas prospect in northern Pecos County, Texas, revealed that portions of this margin may be characterized by shingled, low-angle, eastward-dipping, basement involved thrust faults. Interpretations suggest that hidden, subthrust footwall structures may trend discontinuously for greater than 100 mi along this structural margin. Subthrust footwall structures formed as basinal buttress points for the Central Basin platform to climb over the Delaware basin. In this area, structural relief of over 19,000 ft over a 10-mi width is believed due to stacking of low-angle thrust sheets. Seismic resolution of this subthrust margin has been complexed by allochtonous hanging-wall gravity-glide blocks and folds and by velocity changes in overlying syn- and posttectonic sediments associated with basin-to-shelf lithofacies changes. Statistical studies indicate that this deep-gas province has a play potential of greater than 10 tcf of gas, with individual prospect sizes exceeding 1 tcfg. The prospects defined along this trend are deep (approximately 20,000 ft) subthrust structural traps that are indigenously sourced and reservoired by dual-matrix porosity. Vitrinite supported maturation modeling suggests that these subthrust structures formed prior to catagenic conversion of the oldest source rocks to oil and later to gas. Tectonically fractured Ordovician Ellenburger and Devonian sediments are considered the principal reservoirs. Shales overlying reservoir intervals form vertical seals.

  9. Variant (Swine Origin) Influenza Viruses in Humans

    Science.gov (United States)

    ... Past Newsletters Variant (Swine Origin) Influenza Viruses in Humans Language: English Español Recommend on Facebook Tweet ... Page Background Reporting Additional Information Key Facts about Human Infections with Variant Viruses (Swine Origin Influenza Viruses ...

  10. Zika Virus and Complications: Questions and Answers

    Science.gov (United States)

    ... Feed Youtube Twitter Facebook Google + iTunes Play Store Zika virus and complications: Questions and answers Online Q& ... a cause of microcephaly and Guillain-Barré syndrome. Zika virus Updated! How do people catch Zika virus? ...

  11. Dengue Virus May Bolster Zika's Attack

    Science.gov (United States)

    ... dengue fever virus may increase the severity of Zika virus, a new study says. Early stage laboratory findings ... Services, or federal policy. More Health News on: Zika Virus Recent Health News Related MedlinePlus Health Topics Dengue ...

  12. Chikungunya Virus: What You Need to Know

    Science.gov (United States)

    Chikungunya Virus: What you need to know Chikungunya (pronunciation: \\chik-en-gun-ye) is: A virus spread through Aedes species mosquito bites. Aedes mosquitoes also spread dengue and Zika viruses. A risk to anyone traveling to a region ...

  13. Rocky Mountain Tertiary coal-basin models and their applicability to some world basins

    Science.gov (United States)

    Flores, R.M.

    1989-01-01

    Tertiary intermontane basins in the Rocky Mountain region of the United States contain large amounts of coal resources. The first major type of Tertiary coal basin is closed and lake-dominated, either mud-rich (e.g., North Park Basin, Colorado) or mud plus carbonate (e.g., Medicine Lodge Basin, Montana), which are both infilled by deltas. The second major type of Tertiary coal basin is open and characterized by a preponderance of sediments that were deposited by flow-through fluvial systems (e.g., Raton Basin, Colorado and New Mexico, and Powder River Basin, Wyoming and Montana). The setting for the formation of these coals varies with the type of basin sedimentation, paleotectonism, and paleoclimate. The mud-rich lake-dominated closed basin (transpressional paleotectonism and warm, humid paleoclimate), where infilled by sandy "Gilbert-type" deltas, contains thick coals (low ash and low sulfur) formed in swamps of the prograding fluvial systems. The mud- and carbonate-rich lake-dominated closed basin is infilled by carbonate precipitates plus coarse-grained fan deltas and fine-grained deltas. Here, thin coals (high ash and high sulfur) formed in swamps of the fine-grained deltas. The coarse-clastic, open basins (compressional paleotectonism and warm, paratropical paleoclimate) associated with flow-through fluvial systems contain moderately to anomalously thick coals (high to low ash and low sulfur) formed in swamps developed in intermittently abandoned portions of the fluvial systems. These coal development patterns from the Tertiary Rocky Mountain basins, although occurring in completely different paleotectonic settings, are similar to that found in the Tertiary, Cretaceous, and Permian intermontane coal basins in China, New Zealand, and India. ?? 1989.

  14. Delaware Basin Monitoring Annual Report

    International Nuclear Information System (INIS)

    The Delaware Basin Drilling Surveillance Program (DBDSP) is designed to monitor drilling activities in the vicinity of the Waste Isolation Pilot Plant (WIPP). This program is based on Environmental Protection Agency (EPA) requirements. The EPA environmental standards for the management and disposal of transuranic (TRU) radioactive waste are codified in 40 CFR Part 191 (EPA 1993). Subparts B and C of the standard address the disposal of radioactive waste. The standard requires the Department of Energy (DOE) to demonstrate the expected performance of the disposal system using a probabilistic risk assessment or performance assessment (PA). This PA must show that the expected repository performance will not release radioactive material above limits set by the EPA's standard. This assessment must include the consideration of inadvertent drilling into the repository at some future time.

  15. Bold enterprise in Amazon basin

    Energy Technology Data Exchange (ETDEWEB)

    Johnston, D.

    1980-08-30

    The aim of the Jari project in Brazil is to produce food and forest products for world markets by developing a 15,000 square km tract in the Amazon basin. A pumpmill and power plant came on stream in 1979 and since then have been meeting production targets of high quality bleached pulp. The key to the success of the project has been the introduction of a fast-growing hardwood native to S.E. Asia- Gmelina arborea which reaches a height of 30 m after 10 years, and is suitable for most wood products: pulp, sawn timber, veneer, plywood and particleboard. It is stated that preparations are under-way to introduce Jari hardwood to European markets.

  16. Submarine Landslides in Arctic Sedimentation: Canada Basin

    Science.gov (United States)

    Mosher, David C.; Shimeld, John; Hutchinson, Deborah R.; Lebedova-Ivanova, N; Chapman, C.

    2016-01-01

    Canada Basin of the Arctic Ocean is the least studied ocean basin in the World. Marine seismic field programs were conducted over the past 6 years using Canadian and American icebreakers. These expeditions acquired more than 14,000 line-km of multibeam bathymetric and multi-channel seismic reflection data over abyssal plain, continental rise and slope regions of Canada Basin; areas where little or no seismic reflection data existed previously. Canada Basin is a turbidite-filled basin with flat-lying reflections correlateable over 100s of km. For the upper half of the sedimentary succession, evidence of sedimentary processes other than turbidity current deposition is rare. The Canadian Archipelago and Beaufort Sea margins host stacked mass transport deposits from which many of these turbidites appear to derive. The stratigraphic succession of the MacKenzie River fan is dominated by mass transport deposits; one such complex is in excess of 132,000 km2 in area and underlies much of the southern abyssal plain. The modern seafloor is also scarred with escarpments and mass failure deposits; evidence that submarine landsliding is an ongoing process. In its latest phase of development, Canada Basin is geomorphologically confined with stable oceanographic structure, resulting in restricted depositional/reworking processes. The sedimentary record, therefore, underscores the significance of mass-transport processes in providing sediments to oceanic abyssal plains as few other basins are able to do.

  17. Computer virus information update CIAC-2301

    Energy Technology Data Exchange (ETDEWEB)

    Orvis, W.J.

    1994-01-15

    While CIAC periodically issues bulletins about specific computer viruses, these bulletins do not cover all the computer viruses that affect desktop computers. The purpose of this document is to identify most of the known viruses for the MS-DOS and Macintosh platforms and give an overview of the effects of each virus. The authors also include information on some windows, Atari, and Amiga viruses. This document is revised periodically as new virus information becomes available. This document replaces all earlier versions of the CIAC Computer virus Information Update. The date on the front cover indicates date on which the information in this document was extracted from CIAC`s Virus database.

  18. How integrated is river basin management?

    Science.gov (United States)

    Downs, Peter W.; Gregory, Kenneth J.; Brookes, Andrew

    1991-05-01

    Land and water management is increasingly focused upon the drainage basin. Thirty-six terms recently used for schemes of “integrated basin management” include reference to the subject or area and to the aims of integrated river basin management, often without allusion to the multiobjective nature. Diversity in usage of terms has occurred because of the involvement of different disciplines, of the increasing coherence of the drainage basin approach, and the problems posed in particular parts of the world. The components included in 21 different approaches are analyzed, and, in addition to showing that components related broadly to water supply, river channel, land, and leisure aspects, it is concluded that there are essentially five interrelated facets of integrated basin management that involved water, channel, land, ecology, and human activity. Two aspects not fully included in many previous schemes concern river channel changes and the dynamic integrity of the fluvial system. To clarify the terminology used, it is suggested that the term comprehensive river basin management should be used where a wide range of components is involved, whereas integrated basin management can signify the interactions of components and the dominance of certain components in the particular area. Holistic river basin management is advocated as a term representing an approach that is both fully comprehensive and integrated but also embraces the energetics of the river system and consideration of changes of river channels and of human impacts throughout the river system. The paradigm of working with the river can be extended to one of working with the river in the holistic basin context.

  19. Groundwater Mounding Beneath Stormwater Infiltration Basins

    Science.gov (United States)

    Nimmer, M.; Thompson, A. M.; Misra, D.

    2007-12-01

    An accurate understanding of groundwater mound formation is important in the proper design of stormwater infiltration basins since these basins are often required to recharge a portion of pre-development infiltration volume. Mound formation due to localized recharge may reduce the infiltration rate of the basin and the ability of the soil to filter pollutants. The goal of this research was to understand groundwater mounding and the potential for contaminant transport resulting from recharge beneath stormwater infiltration basins. A 0.10 ha infiltration basin serving a 9.4 ha residential subdivision in Oconomowoc, Wisconsin was used in this study. Subsurface conditions included sand and gravel material and a groundwater table at 2.3 m below grade. Three storm events, 4.9 cm, 2.8 cm, and 4.3 cm, between August 2006 and April 2007 were modeled using the two-dimensional numerical model HYDRUS. The calibrated model was used to evaluate hypothetical basin operation scenarios for various basin sizes, soil types, ponding depths, and water table depths. The groundwater mound intersected the basin floor in most scenarios with loamy sand and sandy loam soils, an unsaturated thickness of 1.52 m, and a ponding depth of 0.61 m. No groundwater table response was observed with ponding depths less than 0.31 m with an unsaturated zone thickness of 6.09 m. The mound height was most sensitive to hydraulic conductivity and unsaturated zone thickness. A 7.6 cm sediment layer delayed the time to reach maximum mound height, but had a minimal effect on the magnitude of the mound. Mound heights increased as infiltration basin size increased.

  20. Sturgeon nucleo-cytoplasmic large DNA virus phylogeny and PCR tests.

    Science.gov (United States)

    Clouthier, Sharon C; VanWalleghem, Elissa; Anderson, Eric D

    2015-12-01

    Sturgeon epitheliotropic nucleo-cytoplasmic large DNA viruses (NCLDVs) can cause a lethal disease of the integumentary system. These viruses have not been assigned to any currently recognized family or genus. In this study, phylogenetic analyses using the major capsid protein (MCP) showed that the sturgeon NCLDVs formed a cohesive taxonomic group, could be identified to the species or possibly sub-species level and formed a distinct evolutionary lineage within the Megavirales. The genetic relatedness of the sturgeon virus MCP allowed design of 3 PCR diagnostic tests with analytical specificity (ASp) inclusive of this group of viruses. The conventional PCR test, C1, had broader ASp than the 2 quantitative PCR tests, Q1 and Q2, and was inclusive of the sturgeon viruses as well as some viruses belonging to the families Mimi-, Phycodna-, or Iridoviridae. Q2 had broader specificity than Q1 but both tests recognized the sturgeon NCLDVs and did not cross-react with co-localizing sturgeon herpesviruses. Analytical test performance characteristics evaluated for Q1 and Q2 revealed sensitive assays with observed 50% limits of detection between 3 and 6.25 plasmid copies and high intra- and inter-assay repeatability. Q1 was used to test for sturgeon viruses in endangered populations of lake sturgeon Acipenser fulvescens within the Winnipeg River or Nelson River drainage systems of Manitoba, Canada. Test results indicated that namao virus is endemic in the Nelson River water basin. These tests meet the analytical requirements for diagnostic testing in Canada and are useful tools for disease management in sturgeon conservation stocking programs in North America. PMID:26648102

  1. Zika virus : epidemiology, clinical features and host-virus interactions

    OpenAIRE

    Hamel, Rodolphe; Liégeois, Florian; Wichit, S.; Pompon, J.; Diop, F.; Talignani, L.; Thomas, F; Desprès, P; Yssel, H; Missé, Dorothée

    2016-01-01

    Very recently, Zika virus (ZIKV) has gained a medical importance following the large-scale epidemics in South Pacific and Latin America. This paper reviews information on the epidemiology and clinical features of Zika disease with a particular emphasis on the host-virus interactions that contribute to the pathogenicity of ZIKV in humans.

  2. Radionuclides in the Great Lakes basin.

    OpenAIRE

    Ahier, B A; Tracy, B L

    1995-01-01

    The Great Lakes basin is of radiologic interest due to the large population within its boundaries that may be exposed to various sources of ionizing radiation. Specific radionuclides of interest in the basin arising from natural and artificial sources include 3H, 14C, 90Sr, 129I, 131I, 137Cs, 222Rn, 226Ra, 235U, 238U, 239Pu, and 241Am. The greatest contribution to total radiation exposure is the natural background radiation that provides an average dose of about 2.6 mSv/year to all basin resi...

  3. China's Qaidam Basin Sensitive to Global Warming

    Institute of Scientific and Technical Information of China (English)

    2009-01-01

    @@ Chinese meteorologists recently pointed out that the temperatures in northwest China's resource-rich Qaidam Basin are rising faster than the rest of the country as a result of climate change.The rapid rise in temperatures is likely to increase the long-term frequency of droughts and farming pests in the basin to the northeast of the Qinghai-Tibet Plateau.Qaidam Basin covers 250,000 km2 in Qinghai Province and is known as resource-rich with salt lakes,petroleum,natural gas and other abundant mineral resources.

  4. Basins of Attraction for Chimera States

    DEFF Research Database (Denmark)

    Martens, Erik Andreas; Panaggio, Mark; Abrams, Daniel

    2016-01-01

    Chimera states---curious symmetry-broken states in systems of identical coupled oscillators---typically occur only for certain initial conditions. Here we analyze their basins of attraction in a simple system comprised of two populations. Using perturbative analysis and numerical simulation we...... evaluate asymptotic states and associated destination maps, and demonstrate that basins form a complex twisting structure in phase space. Understanding the basins' precise nature may help in the development of control methods to switch between chimera patterns, with possible technological and neural system...

  5. Rifting Thick Lithosphere - Canning Basin, Western Australia

    Science.gov (United States)

    Czarnota, Karol; White, Nicky

    2016-04-01

    The subsidence histories and architecture of most, but not all, rift basins are elegantly explained by extension of ~120 km thick lithosphere followed by thermal re-thickening of the lithospheric mantle to its pre-rift thickness. Although this well-established model underpins most basin analysis, it is unclear whether the model explains the subsidence of rift basins developed over substantially thick lithosphere (as imaged by seismic tomography beneath substantial portions of the continents). The Canning Basin of Western Australia is an example where a rift basin putatively overlies lithosphere ≥180 km thick, imaged using shear wave tomography. Subsidence modelling in this study shows that the entire subsidence history of the Canning Basin is adequately explained by mild Ordovician extension (β≈1.2) of ~120 km thick lithosphere followed by post-rift thermal subsidence. This is consistent with the established model, described above, albeit with perturbations due to transient dynamic topography support which are expressed as basin-wide unconformities. In contrast the Canning Basin reveals an almost continuous period of normal faulting between the Ordovician and Carboniferous (βCanning Basin to rifting of thick lithosphere beneath the eastern part, verified by the presence of ~20 Ma diamond-bearing lamproites intruded into the basin depocentre. In order to account for the observed subsidence, at standard crustal densities, the lithospheric mantle is required to be depleted in density by 50-70 kg m-3, which is in line with estimates derived from modelling rare-earth element concentrations of the ~20 Ma lamproites and global isostatic considerations. Together, these results suggest that thick lithosphere thinned to > 120 km is thermally stable and is not accompanied by post-rift thermal subsidence driven by thermal re-thickening of the lithospheric mantle. Our results show that variations in lithospheric thickness place a fundamental control on basin architecture

  6. Gravity Analysis of the Jeffera Basin, Tunisia

    Science.gov (United States)

    Mickus, K.; Gabtni, H.; Jallouli, C.

    2004-12-01

    Southern Tunisia consists of two main tectonic provinces: 1) the Saharan Platform and 2) the folded Atlasic domain, separated by the North Saharan Flexure. The Saharan Platform, which contains the Ghadames Basin and the Telemzane Arch, consists of gently dipping Paleozoic strata overlain by Triassic to Cretaceous sediments. The Atlasic domain consists of a thicker sequence of mainly Mesozoic and younger rock with less complete sequences of Paleozoic strata. Within the Atlasic domain are the still actively subsiding Chotts and Jeffera basins. The Jeffera basin, which occurs to the east of the Telemzane Arch contains at least eight kilometers of Paleozoic and younger sediment that were formed during numerous subsidence episodes since Carboniferous time. The Jeffera basin is dominated by tilted fault blocks that were formed during numerous tectonic episodes. Several unpublished seismic reflection profiles and well data exist for the Jeffera basin, however a deep structural analysis of the basin has not been published. We examined the existing gravity data in conjunction with available well and geologic data to determine structural features within the basin. The Bouguer gravity anomaly map shows that the Jeffera basin is dominated by a narrow northwest-trending gravity minimum. However, a more detailed analysis consisting of wavelength filtering and edge enhancements indicate that the structure of the basin is more complicated than indicated by the Bouguer gravity anomaly map. A residual gravity anomaly map indicates that the Jeffera basin consists of at least three and maybe four subbasins. Additionally, the Jeffera Fault marks the boundary between northwest-trending gravity anomalies to its northeast and east-trending anomalies over the Saharan Platform. The above observation is amplified by the construction of the enhanced horizontal derivatives (EHG) of both the complete Bouguer gravity and the residual gravity anomaly maps. The EHG maps highlight the lateral

  7. K Basins isolation barriers summary report

    Energy Technology Data Exchange (ETDEWEB)

    Strickland, G.C., Westinghouse Hanford

    1996-07-31

    The 105-K East and 105-K West fuel storage basins (105-K Basins) were designed and constructed in the early 1950`s for interim storage of irradiated fuel following its discharge from the reactors. The 105-K- East and 105-K West reactor buildings were constructed first, and the associated storage basins were added about a year later. The construction joint between each reactor building structure and the basin structure included a flexible membrane waterstop to prevent leakage. Water in the storage basins provided both radiation shielding and cooling to remove decay heat from stored fuel until its transfer to the Plutonium Uranium Extraction (PUREX) Facility for chemical processing. The 105-K West Reactor was permanently shut down in February 1970; the 105-K East Reactor was permanently shut down in February 1971. Except for a few loose pieces, fuel stored in the basins at that time was shipped to the PUREX Facility for processing. The basins were then left idle but were kept filled with water. The PUREX Facility was shut down and placed on wet standby in 1972 while N Reactor continued to operate. When the N Reactor fuel storage basin began to approach storage capacity, the decision was made to modify the fuel storage basins at 105-K East and 105-K West to provide additional storage capacity. Both basins were subsequently modified (105-K East in 1975 and 105-K West in 1981) to provide for the interim handling and storage of irradiated N Reactor fuel. The PUREX Facility was restarted in November 1983 to provide 1698 additional weapons-grade plutonium for the United States defense mission. The facility was shut down and deactivated in December 1992 when the U.S. Department of Energy (DOE) determined that the plant was no longer needed to support weapons-grade plutonium production. When the PUREX Facility was shut down, approximately 2.1 x 1 06 kg (2,100 metric tons) of irradiated fuel aged 7 to 23 years was left in storage in the 105-K Basins pending a decision on

  8. Epidemiology of West Nile Disease in Europe and in the Mediterranean Basin from 2009 to 2013

    Directory of Open Access Journals (Sweden)

    Daria Di Sabatino

    2014-01-01

    Full Text Available West Nile virus (WNV transmission has been confirmed in the last four years in Europe and in the Mediterranean Basin. An increasing concern towards West Nile disease (WND has been observed due to the high number of human and animal cases reported in these areas confirming the importance of this zoonosis. A new epidemiological scenario is currently emerging: although new introductions of the virus from abroad are always possible, confirming the epidemiological role played by migratory birds, the infection endemisation in some European territories today is a reality supported by the constant reoccurrence of the same strains across years in the same geographical areas. Despite the WND reoccurrence in the Old World, the overwintering mechanisms are not well known, and the role of local resident birds or mosquitoes in this context is poorly understood. A recent new epidemiological scenario is the spread of lineage 2 strain across European and Mediterranean countries in regions where lineage 1 strain is still circulating creating favourable conditions for genetic reassortments and emergence of new strains. This paper summarizes the main epidemiological findings on WNV occurrence in Europe and in the Mediterranean Basin from 2009 to 2013, considering potential future spread patterns.

  9. Western Gas Sands Project. Quarterly Basin Activities Report

    Energy Technology Data Exchange (ETDEWEB)

    Atkinson, C H

    1979-01-31

    This report is a summation of 3 months' drilling and testing activities in the four primary WGSP study areas: Greater Green River Basin, Northern Great Plains Province, Piceance Basin, and Uinta Basin. The monitoring of basin activities is part of resource assessment. (DLC)

  10. Western Gas Sands Project Quarterly Basin Activities Report

    Energy Technology Data Exchange (ETDEWEB)

    Atkinson, C H

    1979-04-30

    This quarterly basin activities report is a summation of three months drilling and testing activities in the Greater Green River Basin, Northern Great Plains Province, Piceance Basin, and Uinta Basin. Detailed information is given for each study area for the first quarter of 1979.

  11. Reverse genetics with animal viruses

    International Nuclear Information System (INIS)

    Full text: Reverse genetics of negative-strand RNA viruses (NSV), which allows generation of recombinant viruses entirely from cloned cDNA, has progressed rapidly in the past decade. NSV are a large and diverse group of enveloped viruses of both medical and veterinary importance. They differ widely in morphology, genome structure and host interactions. The first NSV that was completely amenable to genetic manipulation is the neurotropathogenic rabies virus of the rhabdovirus family. In subsequent years, vesicular stomatitis virus and a number of viruses belonging to the family Paramyxoviridae, including viruses causing important animal diseases such as rinderpest virus, canine distemper virus, bovine respiratory syncytial virus, bovine parainfluenza virus and Newcastle disease virus (NDV), succumbed to genetic engineering. The ability to genetically manipulate NSV opens a wide range of possibilities to study the virus biology and develop improved vaccines. Identification and analysis of attenuating mutations using the recombinant system could lead to generation of safe vaccine strains. Introduction of one of the previously studied mutation into an infectious rabies virus (RV) clone by replacing the arginine at position 333 of RV glycoprotein (G-protein) by an aspartic acid resulted in a dramatic attenuation. Combination of this mutation with a deletion that eliminates the interaction between RV P-protein and the cytoplasmic dynein light chain (LC8), which is presumably involved in retrograde transport of RV, further attenuates the rabies virus by 30-fold after intramuscular inoculation. Since extreme attenuation may adversely affect immunogenicity, reverse genetics was used to introduce an additional Gprotein to the step-wise attenuated RV to increase its effectiveness. The resultant recombinant virus may be helpful in developing a highly safe and effective live RV vaccine for oral immunizations of animals. Reverse genetics of NSV has also helped in providing

  12. Multi-National River Basin Cooperation and Management Case Study: Senegal River Basin

    OpenAIRE

    Ayaa, Patricia

    2012-01-01

    With increasing populations, urbanization and thus increasing demand for water, conflict on International River basins has been increasing over the years which has necessitated formation of International River frameworks to devise means of cooperation among the countries sharing the river basins. The main modes of cooperation in international river basins include allocating the waters of the river to the sharing countries such that each country manages its own water resources, or treating the...

  13. Integrated Geochemistry and Basin Modelling Study of the Bakken Formation, Williston Basin, USA

    OpenAIRE

    Philipp Kuhn; Rolando di Primio; Brian Horsfield; Hill, R.

    2010-01-01

    Integrated Geochemistry and Basin Modelling Study of the Bakken Formation, Williston Basin, USA Kuhn, Philipp P.1; di Primio, Rolando 1; Horsfield, Brian 1 (1) Organic Geochemistry, GFZ Helmholtz Centre Potsdam, Potsdam, Germany. The Mississippian‐Devonian Bakken Formation of the Williston Basin is described as a low permeability continuous petroleum system with a limited loss of generated hydrocarbons to adjacent formations. We investigated the geochemical properties of the Bakken source roc...

  14. Structural Architecture and Evolution of Kumkuli Basin, North Tibet

    Institute of Scientific and Technical Information of China (English)

    He Bizhu; Xu Zhiqin; Jiao Cunli; Cui Junwen; Wang Shenglang; Wang Gonghuai; Li Zhaoyang; Qiu Zhuli

    2009-01-01

    Utilizing the new data of gravity, magnetic, and magnetotelluric survey, we analyzed the characteristics of the three geophysical attribute (gravity, magnetic, and resistivity) interfaces and the deep architecture and structure of Kumkuli basin. The research results can provide basic data for early basin structural study. From coupled basin and mountain system, analysis of the structure, and evolution of Knmknli basin, we found that there was zoning from north to south and from west to east. Kumkuli basin has three structural architecture layers including metamorphic crystallization basement, fold basement and sedimentary cover. Knmkuli basin can be divided into three structural units, two depressions, and one uplift. Structural evolution of the Kumkuli basin can be divided into five evolution stages, including Kumkuli microcontinent formed in Sinian-Ordovician, suture around Kumkuli basin formed in Eopaleozoic, retroarc foreland basin formed in Neopaleozoic, rejuvenated foreland hasin developed in Mesozoic, and strike slip and compression basin developed in Cenozoic.

  15. Oncolytic virus therapy for cancer.

    Science.gov (United States)

    Goldufsky, Joe; Sivendran, Shanthi; Harcharik, Sara; Pan, Michael; Bernardo, Sebastian; Stern, Richard H; Friedlander, Philip; Ruby, Carl E; Saenger, Yvonne; Kaufman, Howard L

    2013-01-01

    The use of oncolytic viruses to treat cancer is based on the selection of tropic tumor viruses or the generation of replication selective vectors that can either directly kill infected tumor cells or increase their susceptibility to cell death and apoptosis through additional exposure to radiation or chemotherapy. In addition, viral vectors can be modified to promote more potent tumor cell death, improve the toxicity profile, and/or generate host antitumor immunity. A variety of viruses have been developed as oncolytic therapeutics, including adenovirus, vaccinia virus, herpesvirus, coxsackie A virus, Newcastle disease virus, and reovirus. The clinical development of oncolytic viral therapy has accelerated in the last few years, with several vectors entering clinical trials for a variety of cancers. In this review, current strategies to optimize the therapeutic effectiveness and safety of the major oncolytic viruses are discussed, and a summary of current clinical trials is provided. Further investigation is needed to characterize better the clinical impact of oncolytic viruses, but there are increasing data demonstrating the potential promise of this approach for the treatment of human and animal cancers.

  16. Ebola Virus-Related Encephalitis.

    Science.gov (United States)

    de Greslan, Thierry; Billhot, Magali; Rousseau, Claire; Mac Nab, Christine; Karkowski, Ludovic; Cournac, Jean-Marie; Bordes, Julien; Gagnon, Nicolas; Dubrous, Philippe; Duron, Sandrine; Moroge, Sophie; Quentin, Benoit; Koulibaly, Fassou; Bompaire, Flavie; Renard, Jean-Luc; Cellarier, Gilles

    2016-10-15

    Ebola patients frequently exhibit behavioral modifications with ideation slowing and aggressiveness, sometimes contrasting with mild severity of Ebola disease. We performed lumbar punctures in 3 patients with this presentation and found Ebola virus in all cerebrospinal fluid samples. This discovery helps to discuss the concept of a specific Ebola virus encephalitis. PMID:27418576

  17. Zika Virus Infection and Microcephaly

    Directory of Open Access Journals (Sweden)

    J. Gordon Millichap

    2016-03-01

    Full Text Available A Task Force established by the Brazil Ministry of Health investigated the possible association of microcephaly with Zika virus infection during pregnancy and a registry for microcephaly cases among women suspected to have had Zika virus infection during pregnancy.

  18. El virus del zika

    OpenAIRE

    Sáez García, Cristina

    2016-01-01

    El propósito de este trabajo es hacer una revisión sobre los datos conocidos actualmente relacionados con la infección por el virus Zika y sus consecuencias a nivel neurológico en el desarrollo fetal. La prevención se vuelve esencial contra esta enfermedad: Al no existir vacuna disponible, la actuación debe centrarse en evitar la picadura del mosquito, especialmente aquellas mujeres embarazadas. Las cifras actuales de infectados, y las de bebés nacidos con microcefalia resultan...

  19. Feline immunodeficiency virus infection.

    Science.gov (United States)

    Pedersen, N C; Yamamoto, J K; Ishida, T; Hansen, H

    1989-05-01

    Feline immunodeficiency virus (FIV) (formerly feline T-lymphotropic lentivirus or FTLV) was first isolated from a group of cats in Petaluma, California in 1986. The virus is a typical lentivirus in gross and structural morphology. It replicates preferentially but not exclusively in feline T-lymphoblastoid cells, where it causes a characteristic cytopathic effect. The major structural proteins are 10, 17 (small gag), 28 (major core), 31 (endonuclease?), 41 (transmembrane?), 52 (core precursor polyprotein), 54/62 (reverse transcriptase?), and 110/130 (major envelope) kilodaltons in size. The various proteins are antigenically distinguishable from those of other lentiviruses, although serum from EIAV-infected horses will cross-react with some FIV antigens. Kittens experimentally infected with FIV manifest a transient (several days to 2 weeks) fever and neutropenia beginning 4 to 8 weeks after inoculation. This is associated with a generalized lymphadenopathy that persists for up to 9 months. Most cats recover from this initial phase of the disease and become lifelong carriers of the virus. Complete recovery does not occur to any extent in nature or in the laboratory setting. One experimentally infected cat died from a myeloproliferative disorder several months after infection. The terminal AIDS-like phase of the illness has been seen mainly in naturally infected cats. It appears a year or more following the initial infection in an unknown proportion of infected animals. FIV has been identified in cats from all parts of the world. It is most prevalent in high density populations of free roaming cats (feral and pet), and is very uncommon in closed purebred catteries. Male cats are twice as likely to become infected as females. Older male cats adopted as feral or stray animals are at the highest risk of infection, therefore. The infection rate among freely roaming cats rises throughout life, and reaches levels ranging from less than 1% to 12% or more depending on the

  20. Comparison between control factors of high quality continental reservoirs in Bohai Bay basin and Ordos basin

    Institute of Scientific and Technical Information of China (English)

    Junmao ZHENG; Jun YOU; Dongbo HE

    2008-01-01

    Bohai Bay basin,a typical extensional rift basin,and Ordos basin,an intra-craton down-warped basin,represent two important basin types in China.Because of their respective features,the main control factors for their high quality reservoirs are different.In Bohai Bay basin,the sandbody shows great variety in types and severely separated distribution and its high quality reservoirs are mostly controlled by the vertical secondary pore and vertical abnormal pressure.On the other hand,the sandbody in Ordos basin features a relative lack of variety,good continuity,indistinct vertical zone,and its reservoirs are mainly controlled bY the diagenetic differentiation caused by different sedimentation inside the sandbody.Therefore,in the Bohai Bay basin,the exploration should be based on the analysis of sedimentary facies and the favorable diagenefic zones and formations.The focus in Ordos basin,however,should be put on the relatively high permeability sandstones in a generally low permeable sandstone background.

  1. BASIN STRUCTURE FROM TWO-DIMENSIONAL SEISMIC REFLECTION DATA, CRAZY MOUNTAINS BASIN, MONTANA

    Energy Technology Data Exchange (ETDEWEB)

    David J. Taylor

    2003-08-01

    Some 140 miles of multichannel seismic reflection data, acquired commercially in the 1970's, were reprocessed by the U.S. Geological Survey in late 2000 and early 2001 to interpret the subsurface geology of the Crazy Mountains Basin, an asymmetric Laramide foreland basin located in south-central Montana. The seismic data indicate that the northwestern basin margin is controlled by a thrust fault that places basement rocks over a thick (22,000 feet) sequence of Paleozoic and Mesozoic sedimentary rocks to the south. From the deep basin trough, Paleozoic through Tertiary rocks slope gently upward to the south and southeast. The northern boundary of the basin, which is not imaged well by the seismic data, appears to be folded over a basement ridge rather than being truncated against a fault plane. Seismic data along the basin margin to the south indicate that several fault controlled basement highs may have been created by thin-skinned tectonics where a series of shallow thrust faults cut Precambrian, Paleozoic, and early Mesozoic rocks, whereas, in contrast, Cretaceous and Tertiary strata are folded. The data are further interpreted to indicate that this fault-bounded asymmetric basin contains several structures that possibly could trap hydrocarbons, provided source rocks, reservoirs, and seals are present. In addition, faults in the deep basin trough may have created enough fracturing to enhance porosity, thus developing ''sweet spots'' for hydrocarbons in basin-centered continuous gas accumulations.

  2. [Classification of viruses by computer].

    Science.gov (United States)

    Ageeva, O N; Andzhaparidze, O G; Kibardin, V M; Nazarova, G M; Pleteneva, E A

    1982-01-01

    The study used the information mass containing information on 83 viruses characterized by 41 markers. The suitability of one of the variants of cluster analysis for virus classification was demonstrated. It was established that certain stages of automatic allotment of viruses into groups by the degree of similarity of their properties end the formation of groups which consist of viruses sufficiently close to each other by their properties and are sufficiently isolated. Comparison of these groups with the classification proposed by the ICVT established their correspondence to individual families. Analysis of the obtained classification system permits sufficiently grounded conclusions to be drawn with regard to the classification position of certain viruses, the classification of which has not yet been completed by the ICVT.

  3. Petroleum Exploration Prospects in Erlian Basin,China

    Institute of Scientific and Technical Information of China (English)

    Fei Baosheng

    1994-01-01

    @@ Erlian Basin is located in the middle prairie,Inner Mongolia Autonomous Region, the north border of China. Teetonieally, Erlian Basin is to the south of Bayinbaoligel Uplift and the north is the Wendouer Temple Uplift. In the east of the basin, there is Daxinganling Uplift and the west is Soulunshan Uplift. The basin covered an area of 100 000 km2, is a Mesozoic Cenozoic graben (rift)basin that evolved on the folded basement of Heveynian geosyncline.

  4. K-Basins S/RIDS

    Energy Technology Data Exchange (ETDEWEB)

    Watson, D.J.

    1997-08-01

    The Standards/Requirements Identification Document (S/RID) is a list of the Environmental, Safety, and Health (ES{ampersand}H) and Safeguards and Security (SAS) standards/requirements applicable to the K Basins facility.

  5. Gulf Coast Basins and Uplifts [gcstructsg

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — These data provide generalized outlines of major basins and uplifts in the Gulf Coast region modified after Plate 2, Principal structural features, Gulf of Mexico...

  6. K-Basins S/RIDS

    Energy Technology Data Exchange (ETDEWEB)

    Watson, D.J.

    1995-09-22

    The Standards/Requirements Identification Document(S/RID) is a list of the Environmental, Safety, and Health (ES&H) and Safeguards and Security (SAS) standards/requirements applicable to the K Basins facility

  7. Klamath Basin Water Rights Place of Use

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — Hydrological Information Products for the Off-Project Water Program of the Klamath Basin Restoration Agreement U.S. Geological Survey Open-File Report 2012-1199...

  8. Ferris coalfield boundary, Hanna Basin, Wyoming (ferbndg)

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — This ArcView shapefile contains a representation of the boundary of the Ferris coalfield in the Hanna Basin, Wyoming. This theme was created specifically for the...

  9. K-Basins S/RIDS

    International Nuclear Information System (INIS)

    The Standards/Requirements Identification Document (S/RID) is a list of the Environmental, Safety, and Health (ES(ampersand)H) and Safeguards and Security (SAS) standards/requirements applicable to the K Basins facility

  10. Cretaceous Onlap, Gulf of Mexico Basin [cretonlapg

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — The maximum extent of Cretaceous onlap is generalized from Plate 3, Structure at the base and subcrop below Mesozoic marine section, Gulf of Mexico Basin (compiled...

  11. KE Basin water dispositioning engineering study

    International Nuclear Information System (INIS)

    This engineering study is a feasibility study of KE Basin water treatment to an acceptable level and dispositioning the treated water to Columbia River, ground through ETF or to air through evaporation

  12. Overflow of Radioactive Water from K Basins

    International Nuclear Information System (INIS)

    This report documents the dose calculations for the postulated K Basin overflow accident using current methods to model the environmental doses for radioactive releases into the Columbia River and the air

  13. USGS Streamgage NHDPlus Version 1 Basins 2011

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — This dataset represents 19,031 basin boundaries and their streamgage locations for the U.S. Geological Survey's (USGS) active and historical streamgages from the...

  14. Closed Basin Division, Colorado : Definite Plan Report

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This plan for the Closed Basin Division project is intended to provide stage development of the project features. Project background, plan of development, economic...

  15. River Basin Standards Interoperability Pilot

    Science.gov (United States)

    Pesquer, Lluís; Masó, Joan; Stasch, Christoph

    2016-04-01

    There is a lot of water information and tools in Europe to be applied in the river basin management but fragmentation and a lack of coordination between countries still exists. The European Commission and the member states have financed several research and innovation projects in support of the Water Framework Directive. Only a few of them are using the recently emerging hydrological standards, such as the OGC WaterML 2.0. WaterInnEU is a Horizon 2020 project focused on creating a marketplace to enhance the exploitation of EU funded ICT models, tools, protocols and policy briefs related to water and to establish suitable conditions for new market opportunities based on these offerings. One of WaterInnEU's main goals is to assess the level of standardization and interoperability of these outcomes as a mechanism to integrate ICT-based tools, incorporate open data platforms and generate a palette of interchangeable components that are able to use the water data emerging from the recently proposed open data sharing processes and data models stimulated by initiatives such as the INSPIRE directive. As part of the standardization and interoperability activities in the project, the authors are designing an experiment (RIBASE, the present work) to demonstrate how current ICT-based tools and water data can work in combination with geospatial web services in the Scheldt river basin. The main structure of this experiment, that is the core of the present work, is composed by the following steps: - Extraction of information from river gauges data in OGC WaterML 2.0 format using SOS services (preferably compliant to the OGC SOS 2.0 Hydrology Profile Best Practice). - Model floods using a WPS 2.0, WaterML 2.0 data and weather forecast models as input. - Evaluation of the applicability of Sensor Notification Services in water emergencies. - Open distribution of the input and output data as OGC web services WaterML, / WCS / WFS and with visualization utilities: WMS. The architecture

  16. THE RIVER BASIN APPROACH IN TOURISM PLANNING

    OpenAIRE

    Slara, Agita

    2005-01-01

    The article describes advantages and disadvantages in tourism planning, using the river basins as background territory and borders. Tourism development planning is taking place according administrative territorial borders till nowadays in Latvia and in other tourism destinations in abroad. According tourist and visitor needs and environmental friendly approach it is more appropriate to use river basins in tourism planning. Tourists are not interested in administrative borders, but in qualitat...

  17. Microbiology of spent nuclear fuel storage basins.

    Science.gov (United States)

    Santo Domingo, J W; Berry, C J; Summer, M; Fliermans, C B

    1998-12-01

    Microbiological studies of spent nuclear fuel storage basins at Savannah River Site (SRS) were performed as a preliminary step to elucidate the potential for microbial-influenced corrosion (MIC) in these facilities. Total direct counts and culturable counts performed during a 2-year period indicated microbial densities of 10(4) to 10(7) cells/ml in water samples and on submerged metal coupons collected from these basins. Bacterial communities present in the basin transformed between 15% and 89% of the compounds present in Biologtrade mark plates. Additionally, the presence of several biocorrosion-relevant microbial groups (i.e., sulfate-reducing bacteria and acid-producing bacteria) was detected with commercially available test kits. Scanning electron microscopy and X-ray spectra analysis of osmium tetroxide-stained coupons demonstrated the development of microbial biofilm communities on some metal coupons submerged for 3 weeks in storage basins. After 12 months, coupons were fully covered by biofilms, with some deterioration of the coupon surface evident at the microscopical level. These results suggest that, despite the oligotrophic and radiological environment of the SRS storage basins and the active water deionization treatments commonly applied to prevent electrochemical corrosion in these facilities, these conditions do not prevent microbial colonization and survival. Such microbial densities and wide diversity of carbon source utilization reflect the ability of the microbial populations to adapt to these environments. The presumptive presence of sulfate-reducing bacteria and acid-producing bacteria and the development of biofilms on submerged coupons indicated that an environment for MIC of metal components in the storage basins may occur. However, to date, there has been no indication or evidence of MIC in the basins. Basin chemistry control and corrosion surveillance programs instituted several years ago have substantially abated all corrosion mechanisms.

  18. Microbiology of spent nuclear fuel storage basins.

    Science.gov (United States)

    Santo Domingo, J W; Berry, C J; Summer, M; Fliermans, C B

    1998-12-01

    Microbiological studies of spent nuclear fuel storage basins at Savannah River Site (SRS) were performed as a preliminary step to elucidate the potential for microbial-influenced corrosion (MIC) in these facilities. Total direct counts and culturable counts performed during a 2-year period indicated microbial densities of 10(4) to 10(7) cells/ml in water samples and on submerged metal coupons collected from these basins. Bacterial communities present in the basin transformed between 15% and 89% of the compounds present in Biologtrade mark plates. Additionally, the presence of several biocorrosion-relevant microbial groups (i.e., sulfate-reducing bacteria and acid-producing bacteria) was detected with commercially available test kits. Scanning electron microscopy and X-ray spectra analysis of osmium tetroxide-stained coupons demonstrated the development of microbial biofilm communities on some metal coupons submerged for 3 weeks in storage basins. After 12 months, coupons were fully covered by biofilms, with some deterioration of the coupon surface evident at the microscopical level. These results suggest that, despite the oligotrophic and radiological environment of the SRS storage basins and the active water deionization treatments commonly applied to prevent electrochemical corrosion in these facilities, these conditions do not prevent microbial colonization and survival. Such microbial densities and wide diversity of carbon source utilization reflect the ability of the microbial populations to adapt to these environments. The presumptive presence of sulfate-reducing bacteria and acid-producing bacteria and the development of biofilms on submerged coupons indicated that an environment for MIC of metal components in the storage basins may occur. However, to date, there has been no indication or evidence of MIC in the basins. Basin chemistry control and corrosion surveillance programs instituted several years ago have substantially abated all corrosion mechanisms

  19. Fractal Basins in the Lorenz Model

    Institute of Scientific and Technical Information of China (English)

    I.Djellit; J.C.Sprott; M. R. Ferchichi

    2011-01-01

    @@ The Lorenz mapping is a discretization of a pair of differential equations.It illustrates the pertinence of compu- tational chaos.We describe complex dynamics, bifurcations, and chaos in the map.Fractal basins are displayed by numerical simulation.%The Lorenz mapping is a discretization of a pair of differential equations. It illustrates the pertinence of computational chaos. We describe complex dynamics, bifurcations, and chaos in the map. Fractal basins are displayed by numerical simulation.

  20. Vector competence of Peruvian mosquitoes (Diptera: Culicidae) for a subtype IIIC virus in the Venezuelan equine encephalomyelitis complex isolated from mosquitoes captured in Peru.

    Science.gov (United States)

    Turell, M J; Dohm, D J; Fernandez, R; Calampa, C; O'Guinn, M L

    2006-03-01

    We evaluated mosquitoes collected in the Amazon Basin, near Iquitos, Peru, for their susceptibility to a subtype IIIC strain of the Venezuelan equine encephalomyelitis complex. This virus had been previously isolated from a pool of mixed Culex vomerifer and Cx. gnomatos captured near Iquitos, Peru, in 1997. After feeding on hamsters with viremias of about 10(8) plaque-forming units of virus per ml, Cx. gnomatos was the most efficient vector. Other species, such as Ochlerotatus fulvus and Psorophora cingulata, although highly susceptible to infection, were not efficient laboratory vectors of this virus due to a significant salivary gland barrier. The Cx. (Culex) species, consisting mostly of Cx. (Cux.) coronator, were nearly refractory to subtype IIIC virus and exhibited both midgut infection as well as salivary gland barriers. Additional studies on biting behavior, mosquito population densities, and vertebrate reservoir hosts of subtype IIIC virus are needed to determine the role that these species play in the maintenance and spread of this virus in the Amazon Basin region.

  1. K basins sludge removal sludge pretreatment system

    International Nuclear Information System (INIS)

    The Spent Nuclear Fuels Program is in the process of planning activities to remove spent nuclear fuel and other materials from the 100-K Basins as a remediation effort for clean closure. The 105 K- East and K-West Basins store spent fuel, sludge, and debris. Sludge has accumulated in the 1 00 K Basins as a result of fuel oxidation and a slight amount of general debris being deposited, by settling, in the basin water. The ultimate intent in removing the sludge and fuel is to eliminate the environmental risk posed by storing fuel at the K Basins. The task for this project is to disposition specific constituents of sludge (metallic fuel) to produce a product stream through a pretreatment process that will meet the requirements, including a final particle size acceptable to the Tank Waste Remediation System (TWRS). The purpose of this task is to develop a preconceptual design package for the K Basin sludge pretreatment system. The process equipment/system is at a preconceptual stage, as shown in sketch ES-SNF-01 , while a more refined process system and material/energy balances are ongoing (all sketches are shown in Appendix C). Thus, the overall process and 0535 associated equipment have been conservatively selected and sized, respectively, to establish the cost basis and equipment layout as shown in sketches ES- SNF-02 through 08

  2. Hydrological Modelling of Ganga River basin.

    Science.gov (United States)

    Anand, J.; Gosain, A. K.; Khosa, R.

    2015-12-01

    Application of a hydrological model, Soil and Water Assessment Tool (SWAT) to the Ganga basin having a total drainage area of around 1.08 M sq. km extending over Tibet, Nepal, India and Bangladesh has been made. The model is calibrated to determine the spatial deviations in runoff at sub-basin level, and to capture the water balance of the river basin. Manual calibration approach was used for calibrating the SWAT model by following multi-step procedure to get to the realistic present situation as close as possible. Simulations were then further made with and without proposed future projects to obtain various scenarios. The various statistical parameters used for the evaluation of the monthly runoff simulation showed that SWAT performed well in mimicking the monthly stream flow for Ganga River basin. The model under predicted the flows in the non-perennial region during non-monsoon season, due to low rainfall and regulated flows and seepage taking place from the reservoirs. The impacts of the interventions, both existing as well as proposed, on the water balance of the basin were evaluated and quantified. The derived results suggest that there is a substantial reduction in overall water resources availability in the study basin on account of the current level of development and further, future developments, as are being proposed, may require a careful study of their potential impact on currently sanctioned water use. The present study showcases that efficacy of the model for simulating the stream flow is admirable.

  3. The thermal environment of Cascadia Basin

    Science.gov (United States)

    Johnson, H. Paul; Hautala, Susan L.; Bjorklund, Tor A.

    2012-07-01

    Located adjacent to the NE Pacific convergent boundary, Cascadia Basin has a global impact well beyond its small geographic size. Composed of young oceanic crust formed at the Juan de Fuca Ridge, igneous rocks underlying the basin are partially insulated from cooling of their initial heat of formation by a thick layer of pelagic and turbidite sediments derived from the adjacent North American margin. The igneous seafloor is eventually consumed at the Cascadia subduction zone, where interactions between the approaching oceanic crust and the North American continental margin are partially controlled by the thermal environment. Within Cascadia Basin, basement topographic relief varies dramatically, and sediments have a wide range of thickness and physical properties. This variation produces regional differences in heat flow and basement temperatures for seafloor even of similar age. Previous studies proposed a north-south thermal gradient within Cascadia Basin, with high geothermal flux and crustal temperatures measured in the heavily sedimented northern portion near Vancouver Island and lower than average heat flux and basement temperatures predicted for the central and southern portions of the basin. If confirmed, this prediction has implications for processes associated with the Cascadia subduction zone, including the location of the "locked zone" of the megathrust fault. Although existing archival geophysical data in the central and southern basin are sparse, nonuniformly distributed, and derived from a wide range of historical sources, a substantial N-S geothermal gradient appears to be confirmed by our present compilation of combined water column and heat flow measurements.

  4. Lunar basin formation and highland stratigraphy

    Science.gov (United States)

    Howard, K. A.; Wilhelms, D. E.; Scott, D. H.

    1974-01-01

    Multiring impact basins, formed after solidification of the lunar crust, account for most or all premare regional deposits and structures expressed in the lunar landscape and for major topographic and gravity variations. A fresh basin has two or more concentric mountain rings, a lineated ejecta blanket, and secondary impact craters. Crackled material on the floor may be impact melt. The ejecta blanket was emplaced at least partly as a ground-hugging flow and was probably hot. A suggested model of basin formation is that the center lifts up and the rings form by inward collapse during evisceration. The resulting basin is shallow and has a central uplift of the mantle. This results in a central gravity high and a ring low. Later flooding by mare basalt has since modified most near side basins. Highland deposits of plains, furrowed and pitted terrain, and various hills, domes, and craters that were interpreted before the Apollo missions as being volcanic can now be interpreted as being basin related.

  5. Estimating tectonic history through basin simulation-enhanced seismic inversion: Geoinformatics for sedimentary basins

    Science.gov (United States)

    Tandon, K.; Tuncay, K.; Hubbard, K.; Comer, J.; Ortoleva, P.

    2004-01-01

    A data assimilation approach is demonstrated whereby seismic inversion is both automated and enhanced using a comprehensive numerical sedimentary basin simulator to study the physics and chemistry of sedimentary basin processes in response to geothermal gradient in much greater detail than previously attempted. The approach not only reduces costs by integrating the basin analysis and seismic inversion activities to understand the sedimentary basin evolution with respect to geodynamic parameters-but the technique also has the potential for serving as a geoinfomatics platform for understanding various physical and chemical processes operating at different scales within a sedimentary basin. Tectonic history has a first-order effect on the physical and chemical processes that govern the evolution of sedimentary basins. We demonstrate how such tectonic parameters may be estimated by minimizing the difference between observed seismic reflection data and synthetic ones constructed from the output of a reaction, transport, mechanical (RTM) basin model. We demonstrate the method by reconstructing the geothermal gradient. As thermal history strongly affects the rate of RTM processes operating in a sedimentary basin, variations in geothermal gradient history alter the present-day fluid pressure, effective stress, porosity, fracture statistics and hydrocarbon distribution. All these properties, in turn, affect the mechanical wave velocity and sediment density profiles for a sedimentary basin. The present-day state of the sedimentary basin is imaged by reflection seismology data to a high degree of resolution, but it does not give any indication of the processes that contributed to the evolution of the basin or causes for heterogeneities within the basin that are being imaged. Using texture and fluid properties predicted by our Basin RTM simulator, we generate synthetic seismograms. Linear correlation using power spectra as an error measure and an efficient quadratic

  6. Permian Basin location recommendation report

    International Nuclear Information System (INIS)

    Candidate study areas are screened from the Palo Duro and Dalhart Basin areas using data obtained from studies to date and criteria and specifications that consider: rock geometry; rock characteristics; human intrusion potential; surface characteristics; and environmental and socioeconomic conditions. Two preferred locations are recommended from among these areas for additional characterization to identify potential National Waste Terminal Storage (NWTS) salt repository sites. One location, in northeastern Deaf Smith County and southeastern Oldham County, is underlain by two salt units that meet the adopted screening specifications. The other location, in northcentral Swisher County, is underlain by one salt unit that meets the adopted screening specifications. Both locations have several favorable features, relative to surrounding areas, and no obviously undesirable characteristics. Both lie wholly on the Southern High Plains surface, are in relatively sparsely populated areas, contain no unique land use conflicts, and comprise large enough geographic areas to provide flexibility in site selection. Data gathered to date indicate that these locations contain salt units sufficient in thickness and in depth for the safe construction and operation of the underground facilities under consideration. 93 references, 34 figures, 6 tables

  7. Ebola Virus Antibodies in Fruit Bats, Bangladesh

    OpenAIRE

    Kevin J Olival; Islam, Ariful; Yu, Meng; Anthony, Simon J.; Jonathan H Epstein; Khan, Shahneaz Ali; Khan, Salah Uddin; Crameri, Gary; Wang, Lin-Fa; Lipkin, W. Ian; Luby, Stephen P.; Daszak, Peter

    2013-01-01

    To determine geographic range for Ebola virus, we tested 276 bats in Bangladesh. Five (3.5%) bats were positive for antibodies against Ebola Zaire and Reston viruses; no virus was detected by PCR. These bats might be a reservoir for Ebola or Ebola-like viruses, and extend the range of filoviruses to mainland Asia.

  8. Safe Computing: An Overview of Viruses.

    Science.gov (United States)

    Wodarz, Nan

    2001-01-01

    A computer virus is a program that replicates itself, in conjunction with an additional program that can harm a computer system. Common viruses include boot-sector, macro, companion, overwriting, and multipartite. Viruses can be fast, slow, stealthy, and polymorphic. Anti-virus products are described. (MLH)

  9. The IFITMs Inhibit Zika Virus Replication

    Directory of Open Access Journals (Sweden)

    George Savidis

    2016-06-01

    Full Text Available Zika virus has emerged as a severe health threat with a rapidly expanding range. The IFITM family of restriction factors inhibits the replication of a broad range of viruses, including the closely related flaviruses West Nile virus and dengue virus. Here, we show that IFITM1 and IFITM3 inhibit Zika virus infection early in the viral life cycle. Moreover, IFITM3 can prevent Zika-virus-induced cell death. These results suggest that strategies to boost the actions and/or levels of the IFITMs might be useful for inhibiting a broad range of emerging viruses.

  10. The IFITMs Inhibit Zika Virus Replication.

    Science.gov (United States)

    Savidis, George; Perreira, Jill M; Portmann, Jocelyn M; Meraner, Paul; Guo, Zhiru; Green, Sharone; Brass, Abraham L

    2016-06-14

    Zika virus has emerged as a severe health threat with a rapidly expanding range. The IFITM family of restriction factors inhibits the replication of a broad range of viruses, including the closely related flaviruses West Nile virus and dengue virus. Here, we show that IFITM1 and IFITM3 inhibit Zika virus infection early in the viral life cycle. Moreover, IFITM3 can prevent Zika-virus-induced cell death. These results suggest that strategies to boost the actions and/or levels of the IFITMs might be useful for inhibiting a broad range of emerging viruses. PMID:27268505

  11. Defining Life: The Virus Viewpoint

    Science.gov (United States)

    Forterre, Patrick

    2010-04-01

    Are viruses alive? Until very recently, answering this question was often negative and viruses were not considered in discussions on the origin and definition of life. This situation is rapidly changing, following several discoveries that have modified our vision of viruses. It has been recognized that viruses have played (and still play) a major innovative role in the evolution of cellular organisms. New definitions of viruses have been proposed and their position in the universal tree of life is actively discussed. Viruses are no more confused with their virions, but can be viewed as complex living entities that transform the infected cell into a novel organism—the virus—producing virions. I suggest here to define life (an historical process) as the mode of existence of ribosome encoding organisms (cells) and capsid encoding organisms (viruses) and their ancestors. I propose to define an organism as an ensemble of integrated organs (molecular or cellular) producing individuals evolving through natural selection. The origin of life on our planet would correspond to the establishment of the first organism corresponding to this definition.

  12. Defective interfering particles of Sindbis virus do not interfere with the homologous virus obtained from persistently infected BHK cells but do interfere with Semliki Forest virus.

    OpenAIRE

    Weiss, B; Schlesinger, S

    1981-01-01

    Defective interfering particles derived from wild-type Sindbis virus no longer interfere with the infectious virus cloned from BHK cells persistently infected with Sindbis virus for 16 months. These particles do interfere with the replication of Semliki Forest virus.

  13. Oncolytic virus therapy for cancer

    Directory of Open Access Journals (Sweden)

    Goldufsky J

    2013-09-01

    Full Text Available Joe Goldufsky,1 Shanthi Sivendran,3 Sara Harcharik,4 Michael Pan,4 Sebastian Bernardo,4 Richard H Stern,5 Philip Friedlander,4 Carl E Ruby,1,2 Yvonne Saenger,4 Howard L Kaufman1,2 Departments of 1Immunology & Microbiology and 2Surgery, Rush University Medical Center, Chicago IL, USA 3Hematology/Oncology Medical Specialists, Lancaster General Health, Lancaster, PA, USA, and Departments of 4Medical Oncology and 5Radiology, Tisch Cancer Institute, The Mount Sinai School of Medicine, New York, NY, USA Abstract: The use of oncolytic viruses to treat cancer is based on the selection of tropic tumor viruses or the generation of replication selective vectors that can either directly kill infected tumor cells or increase their susceptibility to cell death and apoptosis through additional exposure to radiation or chemotherapy. In addition, viral vectors can be modified to promote more potent tumor cell death, improve the toxicity profile, and/or generate host antitumor immunity. A variety of viruses have been developed as oncolytic therapeutics, including adenovirus, vaccinia virus, herpesvirus, coxsackie A virus, Newcastle disease virus, and reovirus. The clinical development of oncolytic viral therapy has accelerated in the last few years, with several vectors entering clinical trials for a variety of cancers. In this review, current strategies to optimize the therapeutic effectiveness and safety of the major oncolytic viruses are discussed, and a summary of current clinical trials is provided. Further investigation is needed to characterize better the clinical impact of oncolytic viruses, but there are increasing data demonstrating the potential promise of this approach for the treatment of human and animal cancers. Keywords: cancer, gene therapy, oncolytic therapy, virus, treatment

  14. Implication of drainage basin parameters of a tropical river basin of South India

    Science.gov (United States)

    Babu, K. J.; Sreekumar, S.; Aslam, Arish

    2016-03-01

    Drainage morphometry provides quantitative description of the drainage system which is an important aspect of the characterisation of watersheds. Chalakudi River is one of the important rivers of the South India which has attracted attention of many environmental scientists recently because of the proposed Athirapally Hydel Project across the river. SRTM (Shuttle Radar Topographic Mission) data were used for preparing DEM (Digital Elevation Model), Aspect Map and Slope Map. Geographical Information System (GIS) was used for the evaluation of linear, areal and relief aspects of morphometric parameters. The study reveals that the terrain exhibits dentritic and trellis pattern of drainage. The Chalakudi River Basin has a total area of 1,448.73 km2 and is designated as seventh-order basin. The drainage density of the basin is estimated as 2.54 and the lower-order streams mostly dominate the basin. The high basin relief indicates high runoff and sediment transport. The elongation ratio of the Chalakudi Basin is estimated as 0.48 and indicates that the shape of the basin is elongated. The development of stream segments in the basin area is more or less effected by rainfall. Relief ratio indicates that the discharge capability of watershed is very high and the groundwater potential is meagre. The low value of drainage density in spite of mountainous relief indicates that the area is covered by dense vegetation and resistant rocks permeated by fractures and joints. These studies are helpful in watershed development planning and wise utilization of natural resources.

  15. Data report: an assessment of the occurrence of human viruses in Long Island aquatic systems

    Energy Technology Data Exchange (ETDEWEB)

    Vaughn, J.M.; Landry, E.F.

    1977-12-01

    A virus survey was conducted in Nassau and Suffolk Counties under the auspices of the federally-funded ''208'' program from June 1976 to June 1977. The survey involved the concentration, enumeration, and identification of human enteroviruses from selected aquatic systems on Long Island including embayments, lakes, creeks, public drinking water supplies, groundwater influenced by wastewater recharge, sanitary landfills, and stormwater recharge basins; and the effluents from secondary and tertiary sewage treatment plants. Enteroviruses were isolated from all systems studied except the public water supply wells. As expected, viruses were most often encountered in the chlorinated effluents of sewage treatment plants. On two separate occasions, wild type Poliovirus was isolated from one of these plants. The limited sampling conducted at each site (1 per month) obviated any extensive interpretation of the data for the purpose of identifying the precise hazard posed by enteric viruses in Long Island waters. Among tentative conclusions were: support for the continued study of recharge of groundwater aquifers via the application of properly treated domestic wastewater to recharge basins; caution regarding placement of private septic systems in saturated zones near surface water bodies; the discharge of sewage effluents into embayments; and the identification of those areas requiring further virological study.

  16. Wave tectono-sedimentary processes in Tarim basin

    Institute of Scientific and Technical Information of China (English)

    JIN Zhijun; ZHANG Yiwei; CHEN Shuping

    2005-01-01

    Based on the unconformities, the formation times of structures, and geological wave filtering applied to basin fills of typical wells around the Manjiaer area in the Tarim basin, the wave tectono-sedimentary processes in the Tarim Basin are discussed. The results of geological wave filtering of basin fills of typical wells show that, in time domain, the evolution of the Tarim basin was controlled by various wave processes with the periods of 740±Ma, 200±Ma, 100±Ma and 30±Ma. The analyses of basin fills, basin subsidence and unconformities show that the trends of the sedimentary facies belts, depocenters, and centers of subsidence migrated and interchanged periodically with periods of 200±Ma. These show the propagation of wave movements in space domain. The wave evolution of the Tarim basin is of significance to understanding the formation of the oil and gas reservoirs in the basin.

  17. A chrono-tectonostratigraphic framework for the Sydney Basin

    Science.gov (United States)

    Mohammad, O. A.; Jones, B. G.; Fergusson, C. L.

    2013-12-01

    The Sydney Basin is a foreland basin forming the southern part of the Bowen-Gunnedah-Sydney Basin system in Eastern Australia. Tectonically the Sydney Basin is regarded as a retro-arc foreland basin that developed through Late Carboniferous to Middle Triassic. Constructing a tectonstratigraphic framework for the basin is difficult due to the complex stratigraphy and poor age control, along with its complex tectonic history. A chrono-tectonostratigraphic framework for the Sydney Basin is presented based on literature synthesis combined with new data analysis. The chrono-tectonostratigraphic framework will provide an up-to-date and easy to follow detailed basin history which can be utilize in the exploration for conventional and unconventional resources in the Sydney Basin.

  18. Petroleum geology of the Palo Duro Basin, Texas Panhandle

    International Nuclear Information System (INIS)

    The Palo Duro Basin, Permian Basin, Texas is an asymmetric, relatively shallow, intracratonic basin in the southern Texas Panhandle filled mostly by Mississippian, Pennsylvanian, and Permian sedimentary rocks. Although deeper and prolific prolific petroleum-producing basins adjoin it on the north (Anadarko Basin), south (Midland Basin), and east (Hardeman Basin), the Palo Duro Basin has produced remarkably small amounts of oil and gas to date. This is all the more noteworthy because the sedimentary sequence and rock types of the basin are similar to those of the adjacent basins. Analyses of the stratigraphic succession and structural configuration of the Palo Duro Basin suggest that adequate reservoir rocks, top-seals, and geologic structures are present. Most of the structures formed early enough to have trapped hydrocarbons if they were migrating in the rock column. Although additional work is under way to properly address the question of the petroleum source rocks, generation, and migration, the general absence of production in the basin may relate to an overall deficiency in hydrocarbon generation within the basin. Geologic information in this report will form part of the basis for further analysis and conclusions on hydrocarbon potential in the Palo Duro Basin

  19. A new looming of Zika virus

    OpenAIRE

    Nirav R. Soni

    2016-01-01

    Zika virus (ZIKV) is a member of the virus family Flaviviridae and the genus Flavivirus, transmitted by daytime-active Aedes mosquitoes, such as A. aegypti. ZIKV will continue to spread and it will be difficult to determine how the virus will spread over time. Sign and symptoms of ZIKAVD (Zika virus disease) were conjunctivitis (red eyes), back pain, birth defect-abnormal brain development known as microcephaly and it is diagnosed through PCR (polymerase chain reaction) and virus isolation fr...

  20. Towards the Epidemiological Modeling of Computer Viruses

    OpenAIRE

    Xiaofan Yang; Lu-Xing Yang

    2012-01-01

    Epidemic dynamics of computer viruses is an emerging discipline aiming to understand the way that computer viruses spread on networks. This paper is intended to establish a series of rational epidemic models of computer viruses. First, a close inspection of some common characteristics shared by all typical computer viruses clearly reveals the flaws of previous models. Then, a generic epidemic model of viruses, which is named as the SLBS model, is proposed. Finally, diverse generalizations of ...

  1. Zika Virus: the Latest Newcomer

    OpenAIRE

    Saiz, Juan-Carlos; Vázquez-Calvo, Ángela; Blázquez, Ana B.; Merino-Ramos, Teresa; Escribano-Romero, Estela; Martín-Acebes, Miguel A.

    2016-01-01

    Since the beginning of this century, humanity has been facing a new emerging, or re-emerging, virus threat almost every year: West Nile, Influenza A, avian flu, dengue, Chikungunya, SARS, MERS, Ebola, and now Zika, the latest newcomer. Zika virus (ZIKV), a flavivirus transmitted by Aedes mosquitoes, was identified in 1947 in a sentinel monkey in Uganda, and later on in humans in Nigeria. The virus was mainly confined to the African continent until it was detected in south-east Asia the 1980’s...

  2. Zika virus: the latest newcomer

    OpenAIRE

    Juan-Carlos eSaiz; Angela eVazquez-Calvo; Ana Belen Blazquez; Teresa eMerino-Ramos; Estela eEscribano-Romero; Martín-Acebes, Miguel A.

    2016-01-01

    Since the beginning of this century, humanity has been facing a new emerging, or re-emerging, virus threat almost every year: West Nile, Influenza A, avian flu, dengue, Chikungunya, SARS, MERS, Ebola, and now Zika, the latest newcomer. Zika virus (ZIKV), a flavivirus transmitted by Aedes mosquitoes, was identified in 1947 in a sentinel monkey in Uganda, and later on in humans in Nigeria. The virus was mainly confined to the African continent until it was detected in south-east Asia the 1980´s...

  3. New insights of Sacbrood virus

    Institute of Scientific and Technical Information of China (English)

    Mingxiao; Ma

    2014-01-01

    <正>Over the last several years,major efforts have been expended to study viral infection of honeybees mainly due to colony losses around the world(Allen M,et al.,1996).It seems that honeybees are infected with numerous viruses mounting to 18so far.Infection may be asymptomatic but could still have adverse effects on the bee and may even cause death resulting in colony collapse.Sacbrood virus(SBV)is the most widely distributed of all honey bee viruses.

  4. Marburg Virus Reverse Genetics Systems

    Directory of Open Access Journals (Sweden)

    Kristina Maria Schmidt

    2016-06-01

    Full Text Available The highly pathogenic Marburg virus (MARV is a member of the Filoviridae family and belongs to the group of nonsegmented negative-strand RNA viruses. Reverse genetics systems established for MARV have been used to study various aspects of the viral replication cycle, analyze host responses, image viral infection, and screen for antivirals. This article provides an overview of the currently established MARV reverse genetic systems based on minigenomes, infectious virus-like particles and full-length clones, and the research that has been conducted using these systems.

  5. Hydrogeology of the West Siberian Basin

    International Nuclear Information System (INIS)

    Nuclear fuel cycle activities of the former Soviet Union (FSU) have resulted in significant contamination of the environment in western Siberia. The radioactive releases to surface waters and the surficial environment from the Mayak site are the largest known in the world. However, they are dwarfed by the amounts of liquid wastes injected into the subsurface at Tomsk and Krasnoyarsk since the early 1960s. This paper provides the status of efforts by Pacific Northwest National Laboratory to quantify the regional hydrogeologic context for potential contaminant migration from areas in western Siberia. The West Siberian Basin is the largest platformal basin and region of low relief on earth. Ground water in the West Siberian Basin is contained in a single geologic structure (i.e., a single basin). Hydrogeologic cross sections indicate that freshwater wedges are present in both unconfined and confined aquifers (as well as in Paleozoic rocks) in the highland regions that rim the basin. The authors developed a 13-layer, finite-element computer model of the West Siberian Basin primarily based on GIS integration of data from geologic studies. The top of the hydrologic system was assumed to coincide with a water table derived from smoothed topography and surface-water occurrences; precipitation supplied the water, and the topographic gradient of the water table supplied the driving force for ground-water flow. The general directions of calculated ground-water flow suggest that (1) the major rivers act as discharge areas, with upwelling below the rivers extending down into the basement rocks; and (2) ground-water divides that penetrate the entire thickness of the model are evident between the major rivers. Their results suggest that contaminants entering the confined aquifer system may eventually migrate to the surface, discharging within major rivers, rather than remaining confined for long travel distances within the basin sediments

  6. Zika virus infections.

    Science.gov (United States)

    de Laval, F; Leparc-Goffart, I; Meynard, J-B; Daubigny, H; Simon, F; Briolant, S

    2016-05-01

    Since its discovery in 1947 in Uganda, the Zika virus (ZIKV) remained in the shadows emerging in 2007 in Micronesia, where hundreds of dengue-like syndromes were reported. Then, in 2013-2014, it was rife in French Polynesia, where the first neurological effects were observed. More recently, its arrival in Brazil was accompanied by an unusually high number of children with microcephaly born to mothers infected with ZIKV during the first trimester of pregnancy. In 2016, the World Health Organization declared ZIKV infection to be a public health emergency and now talks about a ZIKV pandemic. This review aims to summarize the current knowledge about ZIKV infection, successively addressing its transmission, epidemiology, clinical aspects, diagnosis, treatment, and prevention before discussing some perspectives. PMID:27412976

  7. Human immunodeficiency virus endocrinopathy

    Directory of Open Access Journals (Sweden)

    Uma Sinha

    2011-01-01

    Full Text Available Human immunodeficiency virus (HIV endocrinopathy encompasses a broad spectrum of disorders. Almost all the endocrine organs are virtually affected by HIV infection. HIV can directly alter glandular function. More commonly secondary endocrine dysfunction occurs due to opportunistic infections and neoplasms in immunocompromised state. The complex interaction between HIV infection and endocrine system may be manifested as subtle biochemical and hormonal perturbation to overt glandular failure. Antiretroviral therapy as well as other essential medications often result in adverse endocrinal consequences. Apart from adrenal insufficiency, hypogonadism, diabetes and bone loss, AIDS wasting syndrome and HIV lipodystrophy need special reference. Endocrinal evaluation should proceed as in other patients with suspected endocrine dysfunction. Available treatment options have been shown to improve quality of life and long-term mortality in AIDS patients.

  8. Cryptovirology: Virus Approach

    Directory of Open Access Journals (Sweden)

    Shivale Saurabh Anandrao

    2011-08-01

    Full Text Available Traditionally, "Cryptography" is a benediction to information processing and communications, it helps people to store information securely and the private communications over long distances. Cryptovirology is the study of applications of cryptography to build the malicious software. It is an investigation, how modern cryptographic tools and paradigms can be used to strengthen, develop and improve new malicious software attacks. Cryptovirology attacks have been categorized as : give malware enhanced privacy andbe more robust against reverse-engineering, secondly give the attacker enhanced anonymity while communicating with deployed malware. This paper presents the idea of ``Cryptovirology'' which introduce a twist on how cryptography can also be used offensively. Being offensive means, it can be used to mount extortion based attacks that cause loss of access to information, loss of confidentiality, and information leakage, tasks which cryptography usually prevents. Also analyze threats and attacks that misuse of cryptography can cause when combined with fraudulent software (viruses, Trojans. Public-keycryptography is very essential for the attacks that based on cryptovirology. This paper also suggest some of the countermeasures, mechanisms to cope with and prevent such attacks. Even if the attackers actions on the host machine are being monitored, it still cannot be proven beyond reasonable doubt that he or she is the attacker; and it is an “originator-concealing attack”. Evidence should be collected from the “author’s own system which was used for the attack”. These attacks have implications on how the use ofcryptographic tools and techniques should be audited and managed in general purpose computing environments, and imply that access to the cryptographic tools should be in well control of the system(such as API routines. The experimental virus would demonstrate how cryptographic packages can be packed into a small space, which may

  9. Neoplastic transformation of chimpanzee cells induced by adenovirus type 12--simian virus 40 hybrid virus.

    OpenAIRE

    Rhim, J S; Trimmer, R; Arnstein, P; Huebner, R J

    1981-01-01

    The adenovirus 12--simian virus 40 hybrid virus produced neoplastic transformation of chimpanzee skin fibroblasts in vitro. The transformed fibroblasts showed morphological alteration and became permanent lines. The transformed cells contained both adenovirus 12 and simian virus 40 large tumor antigens and were virus producers. However at passage 9, one line (WES) was found to be a nonproducer, producing neither infectious virus nor virus-specific antigen detectable by the complement fixation...

  10. Transfection by DNAs of avian erythroblastosis virus and avian myelocytomatosis virus strain MC29.

    OpenAIRE

    Copeland, N G; Cooper, G M

    1980-01-01

    Chicken embryo fibroblasts and NIH 3T3 mouse cells were transformable by DNAs of chicken cells infected with avian myelocytomatosis virus strain MC29 or with avian erythroblastosis virus. Transfection of chicken cells appeared to require replication of MC29 or avian erythroblastosis virus in the presence of a nontransforming helper virus. In contrast, NIH 3T3 cells transformed by MC29 or avian erythroblastosis virus DNA contained only replication-defective transforming virus genomes.

  11. Varicella-zoster virus vaccine DNA differs from the parental virus DNA.

    OpenAIRE

    Ecker, J R; Hyman, R W

    1981-01-01

    The DNAs of a varicella-zoster virus vaccine and its parental virus were compared by CsCl buoyant density centrifugation and restriction enzyme cleavage analysis. The varicella-zoster virus vaccine DNA showed a heterogeneous buoyant profile and altered restriction enzyme cleavage patterns. These changed properties are probably the result of the accumulation of virus containing defective varicella-zoster virus DNA during extensive cell culture passage of the vaccine virus.

  12. Single Assay Detection of Acute Bee Paralysis Virus, Kashmir Bee Virus and Israeli Acute Paralysis Virus

    DEFF Research Database (Denmark)

    Francis, Roy Mathew; Kryger, Per

    2012-01-01

    A new RT-PCR primer pair designed to identify Acute Bee Paralysis Virus (ABPV), Kashmir Bee Virus (KBV) or Israeli Acute Bee Paralysis Virus (IAPV) of honey bees (Apis mellifera L.) in a single assay is described. These primers are used to screen samples for ABPV, KBV, or IAPV in a single RT-PCR ......-PCR reaction saving time and money. The primers are located in the predicted overlapping gene (pog/ORFX) which is highly conserved across ABPV, KBV, IAPV and other dicistroviruses of social insects. This study has also identified the first case of IAPV in Denmark....

  13. Turbulent Flow Measurement in Vortex Settling Basin

    Directory of Open Access Journals (Sweden)

    Jafar Chapokpour

    2011-12-01

    Full Text Available This paper presents the findings of an experimental study on the three-dimensional turbulent flow field in vortex settling basin. An ADV (Acoustic Doppler Velocity Meter were used to catch 3D velocitycomponents inside the basin. Detailed measurements of time-averaged velocity components, turbulent intensity components and turbulent kinetic energy were determined at different radial sections of chamber. Also the normalized time averaged absolute velocity of 3D components in contour type exhibition were conducted and it was found that the absolute velocity generally is influenced by u component of flow. It trends from high magnitude in basin center to the constant magnitude in basin side wall. The normalized turbulent intensity ofthree components was investigated individually. It was found that intensity of 3D components in vicinity of central air core is higher than other regions, decreasing by moving towards basin sidewall except for the sections that influenced directly by entrance flow jet and sidewall exiting overflow. The results of turbulence kinetic energy also had the same interpretation like turbulence intensity and affected by the same boundary conditions which cover turbulence intensity of 3 velocity components overly.

  14. A geological history of the Turkana Basin.

    Science.gov (United States)

    Feibel, Craig S

    2011-01-01

    The Turkana Basin preserves a long and detailed record of biotic evolution, cultural development, and rift valley geology in its sedimentary strata. Before the formation of the modern basin, Cretaceous fluvial systems, Paleogene lakes, and Oligo-Miocene volcano-sedimentary sequences left fossil-bearing strata in the region. These deposits were in part related to an early system of rift basins that stretched from Sudan to the Indian Ocean. The present-day basin has its origins in Pliocene tectonic developments of the modern rift, with subsidence making room for more than one kilometer of Plio-Pleistocene strata. Much of this sequence belongs to the Omo Group, richly fossiliferous sediments associated with the ancestral Omo River and its tributaries. Modern Lake Turkana has a record stretching back more than 200 thousand years, with earlier lake phases throughout the Plio-Pleistocene. The geologic history of the basin is one of dynamic landscapes responding to environmental influences, including tectonics, volcanic activity and climate.

  15. Satellite altimetry over large hydrological basins

    Science.gov (United States)

    Calmant, Stephane

    2015-04-01

    The use of satellite altimetry for hydrological applications, either it is basin management or hydrological modeling really started with the 21st century. Before, during two decades, the efforts were concentrated on the data processing until a precision of a few decimeters could be achieved. Today, several web sites distribute hundreds of series spread over hundeds of rivers runing in the major basins of the world. Among these, the Amazon basin has been the most widely studied. Satellite altimetry is now routinely used in this transboundary basin to predict discharges ranging over 4 orders of magnitude. In a few years, satellite altimetry should evolve dramatically. This year, we should see the launchs of Jason-3 and that of Sentinel-3A operating in SAR mode. With SAR, the accuracy and resolution of a growing number of measurements should be improved. In 2020, SWOT will provide a full coverage that will join in a unique framework all the previous and forthcoming missions. These technical and thematical evolutions will be illustrated by examples taken in the Amazon and Congo basin.

  16. Peste des Petits Ruminants Virus.

    Science.gov (United States)

    Baron, M D; Diallo, A; Lancelot, R; Libeau, G

    2016-01-01

    Peste des petits ruminants virus (PPRV) causes a severe contagious disease of sheep and goats and has spread extensively through the developing world. Because of its disproportionately large impact on the livelihoods of low-income livestock keepers, and the availability of effective vaccines and good diagnostics, the virus is being targeted for global control and eventual eradication. In this review we examine the origin of the virus and its current distribution, and the factors that have led international organizations to conclude that it is eradicable. We also review recent progress in the molecular and cellular biology of the virus and consider areas where further research is required to support the efforts being made by national, regional, and international bodies to tackle this growing threat. PMID:27112279

  17. Crenarchaeal Viruses: Morphotypes and Genomes,

    DEFF Research Database (Denmark)

    Prangishvili, P.; Basta, P.; Garrett, Roger Antony

    2008-01-01

    not been observed among viruses from the other two domains of life, the Bacteria and the Eukarya. Also the sequences of circular and linear genomes of crenarchaeal viruses are remarkable because the vast majority of predicted genes have no homologs in the public sequence databases. Viruses...... of the Crenarchaeota generally do not cause the lysis of the host cells during release of viral progeny and they persist in infected cells in stable carrier state. In accordance with this rather unsophisticated virus-host relationship, the studies of transcription of genes in rudiviruses and fusellovirus SSV1...... demonstrated a simple transcriptional pattern with minimal temporal control. The replication of viral genomes has not been studied experimentally. Nevertheless, some plausible predictions about possible replication strategies could be made based on specific features of several viral genomes. The comparative...

  18. Archaeal viruses of the sulfolobales

    DEFF Research Database (Denmark)

    Erdmann, Susanne; Garrett, Roger Antony

    2015-01-01

    Infection of archaea with phylogenetically diverse single viruses, performed in different laboratories, has failed to activate spacer acquisition into host CRISPR loci. The first successful uptake of archaeal de novo spacers was observed on infection of Sulfolobus solfataricus P2 with an environm......Infection of archaea with phylogenetically diverse single viruses, performed in different laboratories, has failed to activate spacer acquisition into host CRISPR loci. The first successful uptake of archaeal de novo spacers was observed on infection of Sulfolobus solfataricus P2...... in CRISPR loci of Sulfolobus species from a second coinfecting conjugative plasmid or virus (Erdmann and Garrett, Mol Microbiol 85:1044-1056, 2012; Erdmann et al. Mol Microbiol 91:900-917, 2014). Here we describe, firstly, the isolation of archaeal virus mixtures from terrestrial hot springs...

  19. Validation of Plant Virus Detection

    NARCIS (Netherlands)

    Schadewijk, van A.R.; Meekes, E.T.M.; Verbeek, M.; Verhoeven, J.Th.J.

    2011-01-01

    Validation of test methods is required for laboratories seeking ISO 17025 accreditation. Recently developed manuals help choosing relevant performance characteristics to be studied for qualitative tests common in plant virus detection. For routine testing in certification schemes additional informat

  20. Dengue Virus Infection in Africa

    OpenAIRE

    Amarasinghe, Ananda; Kuritsky, Joel N.; Letson, G. William; Margolis, Harold S

    2011-01-01

    Reported incidence of dengue has increased worldwide in recent decades, but little is known about its incidence in Africa. During 1960–2010, a total of 22 countries in Africa reported sporadic cases or outbreaks of dengue; 12 other countries in Africa reported dengue only in travelers. The presence of disease and high prevalence of antibody to dengue virus in limited serologic surveys suggest endemic dengue virus infection in all or many parts of Africa. Dengue is likely underrecognized and u...

  1. Hepatitis virus vaccines: present status.

    OpenAIRE

    Krugman, S.

    1982-01-01

    During the past decade there has been extraordinary progress toward the development of vaccines for the prevention of type A and type B hepatitis. The successful propagation of hepatitis A virus in cell culture in 1979 was followed by the preparation of experimental live attenuated hepatitis A vaccines that have been shown to induce antibody in marmosets and chimpanzees and protect immunized marmosets against challenge with hepatitis A virus. The first human immunization trials will begin in ...

  2. Hendra Virus Re-visited

    Institute of Scientific and Technical Information of China (English)

    Hume Field

    2009-01-01

    Hendra virus, a novel member of the family Paramyxovirus that has emerged from bats in Australia, causes fatal disease in livestock and humans. Eleven spillover events have been identified since the first description of the virus in 1994, resulting in a total of 37 equine cases and six human cases. All human cases have been attributed to exposure to infected horses; there is no evidence of bat-to-human or human-to-human transmission. Low infectivity and a high case fatality rate are features of Hendra virus infection in both horses and humans. The temporal pattern of spillover events suggests seasonal factors (plausibly be environmental, biological or ecological) as the proximate triggers for spillover. Minimisation of the future occurrence and impact of Hendra virus infections requires an understanding of the ecology of flying foxes, of virus infection dynamics in flying foxes, and of the factors that promote spillover. Management strategies seek to minimize the opportunity for effective contact between bats and horses, and limit potential horse-to-horse and horse-to-human transmission. Incomplete knowledge of the ecology of the virus, of the proximate factors associated with spillover, and the inherent difficulties of effectively managing wild populations, preclude a management approach targeted at bats.

  3. Kinetics of virus production from single cells.

    Science.gov (United States)

    Timm, Andrea; Yin, John

    2012-03-01

    The production of virus by infected cells is an essential process for the spread and persistence of viral diseases, the effectiveness of live-viral vaccines, and the manufacture of viruses for diverse applications. Yet despite its importance, methods to precisely measure virus production from cells are lacking. Most methods test infected-cell populations, masking how individual cells behave. Here we measured the kinetics of virus production from single cells. We combined simple steps of liquid-phase infection, serial dilution, centrifugation, and harvesting, without specialized equipment, to track the production of virus particles from BHK cells infected with vesicular stomatitis virus. Remarkably, cell-to-cell differences in latent times to virus release were within a factor of two, while production rates and virus yields spanned over 300-fold, highlighting an extreme diversity in virus production for cells from the same population. These findings have fundamental and technological implications for health and disease.

  4. Hepatitis C Virus Genotypes

    Directory of Open Access Journals (Sweden)

    Kayhan Azadmanesh

    2005-09-01

    Full Text Available IntroductionHepatitis C virus (HCV is an important cause of chronic liver disease. HCV causes 20% of acute hepatitis cases, 70% of all chronic hepatitis cases, 40% of all cases of liver cirrhosis, 60% of hepatocellular carcinomas, and 30% of liver transplants in Europe(1. It is also recognized as the leading cause of liver transplantation in the world(2. Only 20% of infected individuals will recover from this viral infection, while the rest become chronically infected(3. While the majority of chronically infected individuals never exhibit symptoms, approximately 10-30% of these patients will eventually develop cirrhosis or hepatocellular carcinoma, both of which are associated with significant morbidity and mortality(4.More than 170 million people worldwide are chronically infected with HCV. According to WHO report in 2002, chronic liver diseases were responsible for 1.4 million deaths, including 796,000 due to cirrhosis and 616,000 due to primary liver cancer. At least 20% of these deaths are probably attributable to HCV infection- more than 280,000 deaths(5, 6. The prevalence of chronic HCV infection in general population varies greatly in different parts of the world, being estimated between 0.1 and 5%, with a peak prevalence of 20- 25% in Egypt. HCV prevalence seems to be less than 1% in Iran, which is much lower than most of the neighboring countries(7. HCV was the first virus discovered by molecular cloning method without the direct use of biologic or biophysical methods. This was accomplished by extracting, copying into cDNA, and cloning all the nucleic acid from the plasma of a chimpanzee infected with non- A, non-B hepatitis by contaminated factor XIII concentrate(8. The HCV genome is a positive-sense, singlestranded RNA genome approximately 10 kb long. It has marked similarities to those of members of the genera Pestivirus and Flavivirus. Different HCV isolates from around the world show substantial nucleotide sequence variability

  5. An important form of basin-mountain coupling:Orogenic belt and flank basin

    Institute of Scientific and Technical Information of China (English)

    WANG Erchie

    2004-01-01

    Orogeny is always associated with the erosion and sediments carried by both transverse and longitudinal river systems. Those two river systems transport sediments into foreland or hinterland basin and flank basin. Longitudinal river systems prevail in and around the Tibetan Plateau due to the fact that they flow parallel to the strike of structures within the developing mountain belt. The flank basins surrounded the Tibetan Plateau and adjacent areas are developed in different tectonic settings, including inland,continental margin and deep ocean, most of them containing oil and natural gas. Those basins not only have longer evolution histories than foreland basins but also are more complete in their records of deposition. Coupling of orogeny and flank basin deposition also occurred widely in pre-Cenozoic time, in particular, the coupling between the Qinling orogenic belt and Songpan-Ganzi flysch flank basin is the most distinctive. The evolution of ancient latitudinal rivers derived from the Qinling orogenic belt during periods of mountain building was controlled not only by landforms but also by the lateral extrusion of the crustal fragments.

  6. Ground-water hydraulics of the deep-basin brine aquifer, Palo Duro Basin, Texas panhandle

    International Nuclear Information System (INIS)

    The Deep-Basin Brine aquifer of the Palo Duro Basin (Texas Panhandle) underlies thick Permian bedded evaporites that are being evaluated as a potential high-level nuclear waste isolation repository. Potentiometric surface maps of 5 units of the Deep-Basin Brine aquifer were drawn using drill-stem test (DST) pressure data, which were analyzed by a geostatistical technique (kriging) to smooth the large variation in the data. The potentiometric surface maps indicate that the Deep-Basin Brine aquifer could be conceptually modeled as 5 aquifer units; a Lower Permian (Wolfcamp) aquifer, upper and lower Pennsylvanian aquifers, a pre-Pennsylvanian aquifer, and a Pennsylvanian to Wolfcampian granite-wash aquifer. The hydraulic head maps indicate that ground-water flow in each of the units is west to east with a minor northerly component near the Amarillo Uplift, the northern structural boundary of the basin. The Wolfcamp potentiometric surface indicates the strongest component of northerly flow. Inferred flow direction in Pennsylvanian aquifers is easterly, and in the pre-Pennsylvanian aquifer near its pinch-out in the basin center, flow is inferred to be to the north. In the granite-wash aquifer the inferred flow direction is east across the northern edge of the basin and southeast along the Amarillo Uplift

  7. Geothermal structure of Australia's east coast basins

    Science.gov (United States)

    Danis, C. R.; O'Neill, C.

    2010-12-01

    The east coast sedimentary basins of Australia formed on an active margin of eastern Gondwana, and constitute an important hydrocarbon resource. The 1600km long Sydney-Gunnedah-Bowen Basin (SGBB) is largest east coast basin system, with thick Permian to Jurassic sedimentary successions overlying Palaeozoic basement rocks. The SGBB has been the focus of renewed geothermal exploration interest, however, the thermal state and geothermal potential of the system is largely unconstrained. Geothermal exploration programs require an accurate estimate of subsurface temperature information, in addition to favourable geology, to make informed decisions on potential targe developments. Primarily temperature information comes from downhole measurements, generally non-equilibrated, which are traditionally extrapolated to depth, however such extrapolation does not take into account variations in geological structure or thermal conductivity. Here we import deep 3D geological models into finite element conduction simulations, using the code Underworld, to calculate the deep thermal structure of the basin system. Underworld allows us to incorporate complex, detailed geological architecture models, incorporating different material properties for different layers, with variable temperature and depth-dependent properties. We adopt a fixed top boundary temperature on a variable topographic surface, and vary the bottom surface boundary condition, to converge of models which satisfy equilibrated downhole temperature measurement constraints. We find coal plays an important role in insulating sedimentary basins. Heat refracts around the coal interval and produces elevated temperatures beneath thick sediments, especially where thick coal intervals are present. This workflow has been formalized into an Underworld geothermal model library, enabling model centric computational workflows. Using the imported model architecture from the geology, data can be continuously updated and added to the

  8. Exploration Experience and Problem concerning Deep Basin Gas in the Ordos Basin

    Institute of Scientific and Technical Information of China (English)

    MaXinhua

    2004-01-01

    Deep basin gas (DBG) reservoirs, in view of the characteristics of their main parts containing gas, are a type of subtle stratigraphic lithologic traps. But they have different reservoir-forming principles, especially in the distribution of oil, gas and water. DBG is characterized by gas-water invertion, namely the water located above the gas; however, normal non-anticline subtle reservoirs have normal distribution of gas and water, namely the water located under the gas. The theory of DBG broke the conventional exploration idea that gas is usually found in the high part of reservoir and water is under the gas. So, it provided a wide field and a new idea for the exploration of natural gas. Recently Ben E. Law (2002), in his article entitled "Basin-centered Gas Systems", discussed global DBG systemically. He listed 72 basins or areas containing known or suspected DBG, covering North America, South America, Europe, Asia-Pacific, South Asia, Middle East and Africa. Ordos basin, the Sichuan basin and the Jungar basin in China are presented and assumed to be of very high possibility. In China more attention has been paid to the research and exploration of DBG in the past years. The symposiums on DBG were held twice, in Guangzhou in 1998 and in Xi'an in 2000 respectively. In 2002 in particular, the publication of the book named Deep Basin Gas in China by Professor Wangtao indicated that China has entered a new stage in the research on DBG. Meanwhile, it is more cheering that the exploration of DBG in the Ordos Basin has achieved remarkable success. Therefore, analyzing the exploration experiences and problems regarding the Ordos basin will promote the exploration and research of DBG in China.

  9. Occurrence of norovirus and hepatitis A virus in shellfish

    Directory of Open Access Journals (Sweden)

    VALENTINA TERIO

    2014-06-01

    Full Text Available Norovirus (NoV and hepatitis A virus (HAV are a common cause of gastroenteritis outbreaks associated with consumption of raw shellfish. The majority of NoV infections worldwide are due to geno group II NoVs. The predominant HAV strains belong to sub-genotype IB. A total of 369 bivalve molluscs (294 mussels, 42 clams and 33 oysters from several retail points and harvesting class-A areas of the Adriatic basin in South Italy, North Italy and Albania (Butrinti Lagoon were sampled between 2008-2013. All the samples were screened by a hemi-nested RT-PCR specific for NoV geno group II and by a nested RT-PCR for the VP1/2A region of HAV. NoV RNA was detected in 10,5% of samples and ranged from 3% in 2008 to 85% in 2013. HAV RNA was detected in 32,5% of samples and ranged from 90% in 2008 to 3,1% in 2013. The marked decrease in HAV prevalence may be the related to the vaccine-induced immunity, able to interrupt the ecological cycle of HAV. Monitoring the epidemiology of the virus strains circulating in the fieldis pivotal to develop and assess the efficacy of new control strategies to reduce the risks for public health.

  10. Alluvial basin statistics of the Southwest Principal Aquifers (SWPA) study.

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — SWPA_alvbsn is a vector dataset of alluvial-fill basin statistics for the Southwest United States. Statistics for each basin include physical details such as area,...

  11. Basin and Range Province, Western US, USGS Grids, #1

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — These grid files were used to produce gravity and basin depth maps of the Basin and Range Province, western United States. The maps show gravity values and modeled...

  12. Bathymetry of the Aleutian and Bowers Basin, Bering Sea

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — This coverage contains bathymetric contours for Aleutian Basin and Bowers Basin east of the 1867 Convention Line in the southwestern Bering Sea. Geographic extent...

  13. Geology of the Caloris basin, Mercury: a view from MESSENGER.

    Science.gov (United States)

    Murchie, Scott L; Watters, Thomas R; Robinson, Mark S; Head, James W; Strom, Robert G; Chapman, Clark R; Solomon, Sean C; McClintock, William E; Prockter, Louise M; Domingue, Deborah L; Blewett, David T

    2008-07-01

    The Caloris basin, the youngest known large impact basin on Mercury, is revealed in MESSENGER images to be modified by volcanism and deformation in a manner distinct from that of lunar impact basins. The morphology and spatial distribution of basin materials themselves closely match lunar counterparts. Evidence for a volcanic origin of the basin's interior plains includes embayed craters on the basin floor and diffuse deposits surrounding rimless depressions interpreted to be of pyroclastic origin. Unlike lunar maria, the volcanic plains in Caloris are higher in albedo than surrounding basin materials and lack spectral evidence for ferrous iron-bearing silicates. Tectonic landforms, contractional wrinkle ridges and extensional troughs, have distributions and age relations different from their counterparts in and around lunar basins, indicating a different stress history. PMID:18599772

  14. Basin and Range Province, Western US, USGS Grids #3

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — These grid files were used to produce gravity and basin depth maps of the Basin and Range Province, western United States. The maps show gravity values and modeled...

  15. Basin and Range Province, Western US, USGS Grids #2

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — These grid files were used to produce gravity and basin depth maps of the Basin and Range Province, western United States. The maps show gravity values and modeled...

  16. Zika Virus Infection and Zika Fever: Frequently Asked Questions

    Science.gov (United States)

    ... Frequently asked questions Updated: 25 March 2016 ABOUT ZIKA What is Zika virus infection? Zika virus infection is caused by ... possible to characterize the disease better. How is Zika virus transmitted? Zika virus is transmitted to people ...

  17. Basins of attraction for chimera states

    Science.gov (United States)

    Martens, Erik A.; Panaggio, Mark J.; Abrams, Daniel M.

    2016-02-01

    Chimera states—curious symmetry-broken states in systems of identical coupled oscillators—typically occur only for certain initial conditions. Here we analyze their basins of attraction in a simple system comprised of two populations. Using perturbative analysis and numerical simulation we evaluate asymptotic states and associated destination maps, and demonstrate that basins form a complex twisting structure in phase space. Understanding the basins’ precise nature may help in the development of control methods to switch between chimera patterns, with possible technological and neural system applications.

  18. Megafans of the Northern Kalahari Basin

    Science.gov (United States)

    Wilkinson, M. J.; Miller, R. McG.; Eckardt, F.; Kreslavsky, M. A.

    2016-01-01

    We identify eleven megafans (partial cones of fluvial sediment, >80 km radius) in the northern Kalahari Basin, using several criteria based on VIS and IR remotely sensed data and SRTM-based surface morphology reconstructions. Two other features meet fewer criteria of the form which we class as possible megafans. The northern Kalahari megafans are located in a 1700 km arc around the southern and eastern flanks of the Angola's Bié Plateau, from northern Namibia through northwest Botswana to western Zambia. Three lie in the Owambo subbasin centered on the Etosha Pan, three in the relatively small Okavango rift depression, and five in the Upper Zambezi basin. The population includes the well-known Okavango megafan (150 km), Namibia's Cubango megafan, the largest megafan in the region (350 km long), and the largest nested group (the five major contiguous megafans on the west slopes of the upper Zambezi Valley). We use new, SRTM-based topographic roughness data to discriminate various depositional surfaces within the flat N. Kalahari landscapes. We introduce the concepts of divide megafans, derived megafans, and fan-margin rivers. Conclusions. (i) Eleven megafan cones total an area of 190,000 sq km. (ii) Different controls on megafan size operate in the three component basins: in the Okavango rift structural controls become the prime constraint on megafan length by controlling basin dimensions. Megafans in the other les constricted basins appear to conform to classic relationships fan area, slope, and feeder-basin area. (iii) Active fans occupy the Okavango rift depression with one in the Owambo basin. The rest of the population are relict but recently active fans (surfaces are relict with respect to activity by the feeder river). (iv) Avulsive behavior of the formative river-axiomatic for the evolution of megafans-has resulted in repeated rearrangements of regional drainage, with likely effects in the study area well back into the Neogene. Divide megafans comprise the

  19. The classification of polynomial basins of infinity

    CERN Document Server

    DeMarco, Laura

    2011-01-01

    We consider the problem of classifying the dynamics of complex polynomials $f: \\mathbb{C} \\to \\mathbb{C}$ restricted to their basins of infinity. We synthesize existing combinatorial tools --- tableaux, trees, and laminations --- into a new invariant of basin dynamics we call the pictograph. For polynomials with all critical points escaping to infinity, we obtain a complete description of the set of topological conjugacy classes. We give an algorithm for constructing abstract pictographs, and we provide an inductive algorithm for counting topological conjugacy classes with a given pictograph.

  20. Quantitative nanoscale electrostatics of viruses

    Science.gov (United States)

    Hernando-Pérez, M.; Cartagena-Rivera, A. X.; Lošdorfer Božič, A.; Carrillo, P. J. P.; San Martín, C.; Mateu, M. G.; Raman, A.; Podgornik, R.; de Pablo, P. J.

    2015-10-01

    Electrostatics is one of the fundamental driving forces of the interaction between biomolecules in solution. In particular, the recognition events between viruses and host cells are dominated by both specific and non-specific interactions and the electric charge of viral particles determines the electrostatic force component of the latter. Here we probe the charge of individual viruses in liquid milieu by measuring the electrostatic force between a viral particle and the Atomic Force Microscope tip. The force spectroscopy data of co-adsorbed φ29 bacteriophage proheads and mature virions, adenovirus and minute virus of mice capsids is utilized for obtaining the corresponding density of charge for each virus. The systematic differences of the density of charge between the viral particles are consistent with the theoretical predictions obtained from X-ray structural data. Our results show that the density of charge is a distinguishing characteristic of each virus, depending crucially on the nature of the viral capsid and the presence/absence of the genetic material.Electrostatics is one of the fundamental driving forces of the interaction between biomolecules in solution. In particular, the recognition events between viruses and host cells are dominated by both specific and non-specific interactions and the electric charge of viral particles determines the electrostatic force component of the latter. Here we probe the charge of individual viruses in liquid milieu by measuring the electrostatic force between a viral particle and the Atomic Force Microscope tip. The force spectroscopy data of co-adsorbed φ29 bacteriophage proheads and mature virions, adenovirus and minute virus of mice capsids is utilized for obtaining the corresponding density of charge for each virus. The systematic differences of the density of charge between the viral particles are consistent with the theoretical predictions obtained from X-ray structural data. Our results show that the density of

  1. New aerogeophysical study of the Eurasia Basin and Lomonosov Ridge: Implications for basin development

    DEFF Research Database (Denmark)

    Brozena, J.M.; Childers, V.A.; Lawver, L.A.;

    2003-01-01

    In 1998 and 1999, new aerogeophysical surveys of the Arctic Ocean's Eurasia Basin produced the first collocated gravity and magnetic measurements over the western half of the basin. These data increase the density and extend the coverage of the U.S. Navy acromagnetic data from the 1970s. The new...... Norwegian-Greenland Sea. With the opening of the Labrador Sea, Greenland began similar to200 km of northward movement relative to North America and eventually collided with Svalbard, Ellesmere Island, and the nascent Eurasia ocean basin. Both gravity and magnetic data sets reconstructed to times prior to...

  2. SimBasin: serious gaming for integrated decision-making in the Magdalena-Cauca basin

    Science.gov (United States)

    Craven, Joanne; Angarita, Hector; Corzo, Gerald

    2016-04-01

    The Magdalena-Cauca macrobasin covers 24% of the land area of Colombia, and provides more than half of the country's economic potential. The basin is also home a large proportion of Colombia's biodiversity. These conflicting demands have led to problems in the basin, including a dramatic fall in fish populations, additional flooding (such as the severe nationwide floods caused by the La Niña phenomenon in 2011), and habitat loss. It is generally believed that the solution to these conflicts is to manage the basin in a more integrated way, and bridge the gaps between decision-makers in different sectors and scientists. To this end, inter-ministerial agreements are being formulated and a decision support system is being developed by The Nature Conservancy Colombia. To engage stakeholders in this process SimBasin, a "serious game", has been developed. It is intended to act as a catalyst for bringing stakeholders together, an illustration of the uncertainties, relationships and feedbacks in the basin, and an accessible introduction to modelling and decision support for non-experts. During the game, groups of participants are led through a 30 year future development of the basin, during which they take decisions about the development of the basin and see the impacts on four different sectors: agriculture, hydropower, flood risk, and environment. These impacts are displayed through seven indicators, which players should try to maintain above critical thresholds. To communicate the effects of uncertainty and climate variability, players see the actual value of the indicator and also a band of possible values, so they can see if their decisions have actually reduced risk or if they just "got lucky". The game works as a layer on top of a WEAP water resources model of the basin, adapted from a basin-wide model already created, so the fictional game basin is conceptually similar to the Magdalena-Cauca basin. The game is freely available online, and new applications are being

  3. Advances in Research of Garlic Virus Diseases

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    Garlic virus infection is an important disease which affects garlic production,with the increasing years of planting,harm of virus is serious year by year,which seriously affect yield and quality of garlic.In order to know the garlic virus effectively,the paper reviewed the research situation of several important garlic virus in virus species,origin,distribution,host range,symptom,route of transmission,classification,genome and detection technique and the prevention technology of garlic viruses.At the same ...

  4. The Mode of Net-Virus Actions

    Institute of Scientific and Technical Information of China (English)

    ShanXiuming; LiYing; JiaoJian; LiuYang; RenYong; QiuBen; CaoYiqun

    2004-01-01

    The computer network is not only of benefit to people, but also helpful for the spreading of viruses. The net-viruses spread more widely and rapidly than the traditional viruses, and network has become the major path of the virus spreading. The author analyzes the mode of the net-virus spreading, including E-mail spreading, positive scanning spreading and through-server spreading. Then some defense strategies in the anti-virus field are introduced, and some countermeasures of the net computer users are discussed.

  5. The IFITMs Inhibit Zika Virus Replication

    OpenAIRE

    George Savidis; Jill M. Perreira; Jocelyn M. Portmann; Paul Meraner; Zhiru Guo; Sharone Green; Abraham L. Brass

    2016-01-01

    Zika virus has emerged as a severe health threat with a rapidly expanding range. The IFITM family of restriction factors inhibits the replication of a broad range of viruses, including the closely related flaviruses West Nile virus and dengue virus. Here, we show that IFITM1 and IFITM3 inhibit Zika virus infection early in the viral life cycle. Moreover, IFITM3 can prevent Zika-virus-induced cell death. These results suggest that strategies to boost the actions and/or levels of the IFITMs mig...

  6. Unusual Influenza A Viruses in Bats

    Directory of Open Access Journals (Sweden)

    Andrew Mehle

    2014-09-01

    Full Text Available Influenza A viruses infect a remarkably diverse number of hosts. Two completely new influenza A virus subtypes were recently discovered in bats, dramatically expanding the host range of the virus. These bat viruses are extremely divergent from all other known strains and likely have unique replication cycles. Phylogenetic analysis indicates long-term, isolated evolution in bats. This is supported by a high seroprevalence in sampled bat populations. As bats represent ~20% of all classified mammals, these findings suggests the presence of a massive cryptic reservoir of poorly characterized influenza A viruses. Here, we review the exciting progress made on understanding these newly discovered viruses, and discuss their zoonotic potential.

  7. Towards the Epidemiological Modeling of Computer Viruses

    Directory of Open Access Journals (Sweden)

    Xiaofan Yang

    2012-01-01

    Full Text Available Epidemic dynamics of computer viruses is an emerging discipline aiming to understand the way that computer viruses spread on networks. This paper is intended to establish a series of rational epidemic models of computer viruses. First, a close inspection of some common characteristics shared by all typical computer viruses clearly reveals the flaws of previous models. Then, a generic epidemic model of viruses, which is named as the SLBS model, is proposed. Finally, diverse generalizations of the SLBS model are suggested. We believe this work opens a door to the full understanding of how computer viruses prevail on the Internet.

  8. Morphometric analysis of Suketi river basin, Himachal Himalaya, India

    Indian Academy of Sciences (India)

    Anil M Pophare; Umesh S Balpande

    2014-10-01

    Suketi river basin is located in the Mandi district of Himachal Pradesh, India. It encompasses a central inter-montane valley and surrounding mountainous terrain in the Lower Himachal Himalaya. Morphometric analysis of the Suketi river basin was carried out to study its drainage characteristics and overall groundwater resource potential. The entire Suketi river basin has been divided into five sub-basins based on the catchment areas of Suketi trunk stream and its major tributaries. Quantitative assessment of each sub-basin was carried out for its linear, areal, and relief aspects. The analysis reveals that the drainage network of the entire Suketi river basin constitutes a 7th order basin. Out of five sub-basins, Kansa khad sub-basin (KKSB), Gangli khad sub-basin (GKSB) and Ratti khad sub-basin (RKSB) are 5th order subbasins. The Dadour khad sub-basin (DKSB) is 6th order sub-basin, while Suketi trunk stream sub-basin (STSSB) is a 7th order sub-basin. The entire drainage basin area reflects late youth to early mature stage of development of the fluvial geomorphic cycle, which is dominated by rain and snow fed lower order streams. It has low stream frequency (Fs) and moderate drainage density (Dd) of 2.69 km/km2. Bifurcation ratios (Rb) of various stream orders indicate that streams up to 3rd order are surging through highly dissected mountainous terrain, which facilitates high overland flow and less recharge into the subsurface resulting in low groundwater potential in the zones of 1st, 2nd, and 3rd order streams of the Suketi river basin. The circulatory ratio (Rc) of 0.65 and elongation ratio (Re) of 0.80 show elongated nature of the Suketi river basin, while infiltration number (If) of 10.66 indicates dominance of relief features and low groundwater potential in the high altitude mountainous terrain. The asymmetry factor (Af) of Suketi river basin indicates that the palaeo-tectonic tilting, at drainage basin scale, was towards the downstream right side of the

  9. K West basin isolation barrier leak rate test

    Energy Technology Data Exchange (ETDEWEB)

    Whitehurst, R.; McCracken, K.; Papenfuss, J.N.

    1994-10-31

    This document establishes the procedure for performing the acceptance test on the two isolation barriers being installed in K West basin. This acceptance test procedure shall be used to: First establish a basin water loss rate prior to installation of the two isolation barriers between the main basin and the discharge chute in K-Basin West. Second, perform an acceptance test to verify an acceptable leakage rate through the barrier seals.

  10. K West basin isolation barrier leak rate test

    International Nuclear Information System (INIS)

    This document establishes the procedure for performing the acceptance test on the two isolation barriers being installed in K West basin. This acceptance test procedure shall be used to: First establish a basin water loss rate prior to installation of the two isolation barriers between the main basin and the discharge chute in K-Basin West. Second, perform an acceptance test to verify an acceptable leakage rate through the barrier seals

  11. Hydronomics and terranomics in the Nyando basin of Western Kenya.

    OpenAIRE

    Onyango, Leah; Swallow, Brent; Meinzen-Dick, Ruth

    2005-01-01

    This paper uses the concepts of hydronomics as systems of rules that define water management and terranomics as systems of rules that define land management and explores their linkages in rainfed agriculture and irrigation areas in the Nyando basin. The upper reaches of the basin have experienced a change from large scale commercial farming to more intensive small holder farming while in the flood prone lower reaches of the basin several irrigation schemes have been set up. The basin has a co...

  12. Presence and Distribution of Tobacco Viruses in Montenegro

    OpenAIRE

    Jelena Zindović; Nataša Dukić; Aleksandra Bulajić; Jelena Latinović; Ivana Đekić; Bojan Duduk; Branka Krstić

    2007-01-01

    Seven important tobacco viruses were investigated in Montenegro in 2005: Tobacco Mosaic Virus (TMV), Tomato Spotted Wilt Virus (TSWV), Cucumber Mosaic Virus (CMV), Potato Virus Y (PVY), Alfalfa Mosaic Virus (AMV), Tobacco Ring Spot Virus (TRSV) and Potato Virus X(PVX). This investigation included sample collection from four tobacco growing regions in Montenegro and their serological testing by DAS-ELISA test. Presence of different strains of PVY was investigated as well using DAS ELISA test w...

  13. Production of cattle immunotolerant to bovine viral diarrhea virus.

    OpenAIRE

    McClurkin, A W.; Littledike, E T; Cutlip, R C; Frank, G H; Coria, M F; Bolin, S R

    1984-01-01

    Inoculation of bovine virus diarrhea virus into 58 to 125 day old fetuses of bovine virus diarrhea virus seropositive pregnant cows, or inoculation of bovine virus diarrhea virus into seronegative cows 42 to 114 days pregnant, may produce clinically normal calves which are persistently infected with the specific isolate of bovine virus diarrhea virus yet seronegative to the homologous and heterologous isolates. Reinoculation of these persistently infected cattle with their homologous isolate ...

  14. Hydrology of the Johnson Creek Basin, Oregon

    Science.gov (United States)

    Lee, Karl K.; Snyder, Daniel T.

    2009-01-01

    The Johnson Creek basin is an important resource in the Portland, Oregon, metropolitan area. Johnson Creek forms a wildlife and recreational corridor through densely populated areas of the cities of Milwaukie, Portland, and Gresham, and rural and agricultural areas of Multnomah and Clackamas Counties. The basin has changed as a result of agricultural and urban development, stream channelization, and construction of roads, drains, and other features characteristic of human occupation. Flooding of Johnson Creek is a concern for the public and for water management officials. The interaction of the groundwater and surface-water systems in the Johnson Creek basin also is important. The occurrence of flooding from high groundwater discharge and from a rising water table prompted this study. As the Portland metropolitan area continues to grow, human-induced effects on streams in the Johnson Creek basin will continue. This report provides information on the groundwater and surface-water systems over a range of hydrologic conditions, as well as the interaction these of systems, and will aid in management of water resources in the area. High and low flows of Crystal Springs Creek, a tributary to Johnson Creek, were explained by streamflow and groundwater levels collected for this study, and results from previous studies. High flows of Crystal Springs Creek began in summer 1996, and did not diminish until 2000. Low streamflow of Crystal Springs Creek occurred in 2005. Flow of Crystal Springs Creek related to water-level fluctuations in a nearby well, enabling prediction of streamflow based on groundwater level. Holgate Lake is an ephemeral lake in Southeast Portland that has inundated residential areas several times since the 1940s. The water-surface elevation of the lake closely tracked the elevation of the water table in a nearby well, indicating that the occurrence of the lake is an expression of the water table. Antecedent conditions of the groundwater level and autumn

  15. Oncolytic virus therapies.

    Science.gov (United States)

    Buonaguro, Franco Maria; Tornesello, Maria Lina; Izzo, Francesco; Buonaguro, Luigi

    2012-11-01

    Oncolytic virus (OV) therapy currently represents one of the most promising approaches to cancer treatment for their dual anticancer mechanisms: direct lysis of cancer cells (oncolytic feature) and activation of the immunosystem (cancer vaccine aspect). The latter demonstrates the advantage of a multi-target approach against multiple tumor-associated antigens. Since the 2005 SFDA (the Chinese FDA) approval for the clinical use of Oncorine™, the first human OV-based cancer treatment, more than 200 patents have been filed worldwide and several Phase I/II studies have been conducted. This patent review analyzes patents and clinical studies of the most promising OV products to highlight the pros and cons of this innovative anticancer approach, which is currently being tested in several cancers (i.e., hepatocellular carcinoma, melanoma and glioblastoma) by systemic as well as intratumoral injection. Clinical results, although effective only for a limited period of time, are encouraging. Combined treatments with radio or chemotherapeutic protocols are also in progress. PMID:24236929

  16. Hepatitis B virus morphogenesis

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    The hepatitis B virus (HBV) particle consists of an envelope containing three related surface proteins and probably lipid and an icosahedral nucleocapsid of approximately 30 nm diameter enclosing the viral DNA genome and DNA polymerase. The capsid is formed in the cytosol of the infected cell during packaging of an RNA pregenome replication complex by multiple copies of a 21-kDa C protein. The capsid gains the ability to bud during synthesis of the viral DNA genome by reverse transcription of the pregenome in the lumen of the particle. The three envelope proteins S,M, and L shape a complex transmembrane fold at the endoplasmic reticulum, and form disulfide-linked homoand heterodimers. The transmembrane topology of a fraction of the large envelope protein L changes posttranslationally, therefore, the N terminal domain of L (preS) finally appears on both sides of the membrane.During budding at an intracellular membrane, a short linear domain in the cytosolic preS region interacts with binding sites on the capsid surface. The virions are subsequently secreted into the blood. In addition, the surface proteins can bud in the absence of capsids and form subviral lipoprotein particles of 20 nm diameter which are also secreted.

  17. Hepatitis C virus proteins

    Institute of Scientific and Technical Information of China (English)

    Jean Dubuisson

    2007-01-01

    Hepatitis C virus (HCV) encodes a single polyprotein,which is processed by cellular and viral proteases to generate 10 polypeptides. The HCV genome also contains an overlapping +1 reading frame that may lead to the synthesis of an additional protein. Until recently,studies of HCV have been hampered by the lack of a productive cell culture system. Since the identification of HCV genome approximately 17 years ago, structural,biochemical and biological information on HCV proteins has mainly been obtained with proteins produced by heterologous expression systems. In addition, some functional studies have also been confirmed with replicon systems or with retroviral particles pseudotyped with HCV envelope glycoproteins. The data that have accumulated on HCV proteins begin to provide a framework for understanding the molecular mechanisms involved in the major steps of HCV life cycle. Moreover,the knowledge accumulated on HCV proteins is also leading to the development of antiviral drugs among which some are showing promising results in early-phase clinical trials. This review summarizes the current knowledge on the functions and biochemical features of HCV proteins.

  18. Hepatitis B virus replication

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    Hepadnaviruses, including human hepatitis B virus (HBV), replicate through reverse transcription of an RNA intermediate, the pregenomic RNA (pgRNA). Despite this kinship to retroviruses, there are fundamental differences beyond the fact that hepadnavirions contain DNA instead of RNA. Most peculiar is the initiation of reverse transcription: it occurs by protein-priming, is strictly committed to using an RNA hairpin on the pgRNA,ε, as template, and depends on cellular chaperones;moreover, proper replication can apparently occur only in the specialized environment of intact nucleocapsids.This complexity has hampered an in-depth mechanistic understanding. The recent successful reconstitution in the test tube of active replication initiation complexes from purified components, for duck HBV (DHBV),now allows for the analysis of the biochemistry of hepadnaviral replication at the molecular level. Here we review the current state of knowledge at all steps of the hepadnaviral genome replication cycle, with emphasis on new insights that turned up by the use of such cellfree systems. At this time, they can, unfortunately,not be complemented by three-dimensional structural information on the involved components. However, at least for the s RNA element such information is emerging,raising expectations that combining biophysics with biochemistry and genetics will soon provide a powerful integrated approach for solving the many outstanding questions. The ultimate, though most challenging goal,will be to visualize the hepadnaviral reverse transcriptase in the act of synthesizing DNA, which will also have strong implications for drug development.

  19. Ebola virus: recommendations

    CERN Multimedia

    CERN Medical Service

    2014-01-01

    The CERN Medical Service has been closely following, in particular via the WHO, the development of the Ebola virus outbreak currently affecting some African countries. This infectious disease may be passed on through direct contact with the bodily fluids of a sick person.   Based on the recommendations of the WHO and the two Host States, Switzerland and France, as updated on their respective websites, so far there has been no ban on travel to the countries concerned. However, unless it is absolutely essential, you are advised not to visit any of the countries affected by Ebola (Guinea, Republic of Sierra Leone, Liberia, Nigeria). The two Host States have established an alert system, and a check is carried out on departure from the airports of those countries. It is strongly recommended that you contact the Medical Service if you are travelling to those countries. We remind you to observe the basic rules of hygiene such as frequent hand washing, whatever your destination. The Medical Service is...

  20. Riddled Basins of Attraction for Synchronized Type-I Intermittency

    DEFF Research Database (Denmark)

    Mancher, Martin; Nordahn, Morten; Mosekilde, Erik;

    1998-01-01

    Chaotic mortion resticted to an invariant subspace of total phase space may be associated with basins of attraction that are riddled with holes belonging to the basin of another limiting state. We study the emergence of such basins of two coupled one-dimensional maps, each exhibiting type...

  1. The fate of evaporated water from the Ganges basin

    NARCIS (Netherlands)

    Tuinenburg, O.A.; Hutjes, R.W.A.; Kabat, P.

    2012-01-01

    This research studies river basin moisture recycling rates in order to determine the atmospheric part of the water cycle and the influence of the land surface there on. For river basins in India (Ganges and Indus), the fraction of evaporation that falls again as precipitation in the same river basin

  2. New Progress in Petroleum Exploration of Tarim Basin

    Institute of Scientific and Technical Information of China (English)

    Liang Digang

    1994-01-01

    @@ Basin Situation Tarim Basin located in the northwest of China, is one of the few largest basins in the world that haven't been well-explored, covering an area of 560 000km2, a little bit larger than that of France.

  3. 75 FR 33238 - Basin Electric Power Cooperative: Deer Creek Station

    Science.gov (United States)

    2010-06-11

    ...; ] DEPARTMENT OF AGRICULTURE Rural Utilities Service Basin Electric Power Cooperative: Deer Creek Station AGENCY... Basin Electric Power Cooperative's (Basin Electric) application for a RUS loan and a Western... proposed Project is to serve increased load demand for electric power in the eastern portion of...

  4. Detailed bathymetric surveys in the central Indian Basin

    Digital Repository Service at National Institute of Oceanography (India)

    Kodagali, V.N.; KameshRaju, K.A.; Ramprasad, T.; George, P.; Jaisankar, S.

    Over 420,000 line kilometers of echo-sounding data was collected in the Central Indian Basin. This data was digitized, merged with navigation data and a detailed bathymetric map of the Basin was prepared. The Basin can be broadly classified...

  5. Immune based computer virus detection approaches

    Institute of Scientific and Technical Information of China (English)

    TAN Ying; ZHANG Pengtao

    2013-01-01

    The computer virus is considered one of the most horrifying threats to the security of computer systems worldwide.The rapid development of evasion techniques used in virus causes the signature based computer virus detection techniques to be ineffective.Many novel computer virus detection approaches have been proposed in the past to cope with the ineffectiveness,mainly classified into three categories:static,dynamic and heuristics techniques.As the natural similarities between the biological immune system (BIS),computer security system (CSS),and the artificial immune system (AIS) were all developed as a new prototype in the community of anti-virus research.The immune mechanisms in the BIS provide the opportunities to construct computer virus detection models that are robust and adaptive with the ability to detect unseen viruses.In this paper,a variety of classic computer virus detection approaches were introduced and reviewed based on the background knowledge of the computer virus history.Next,a variety of immune based computer virus detection approaches were also discussed in detail.Promising experimental results suggest that the immune based computer virus detection approaches were able to detect new variants and unseen viruses at lower false positive rates,which have paved a new way for the anti-virus research.

  6. Acute otitis media and respiratory virus infections.

    Science.gov (United States)

    Ruuskanen, O; Arola, M; Putto-Laurila, A; Mertsola, J; Meurman, O; Viljanen, M K; Halonen, P

    1989-02-01

    We studied the association of acute otitis media with different respiratory virus infections in a pediatric department on the basis of epidemics between 1980 and 1985. Altogether 4524 cases of acute otitis media were diagnosed. The diagnosis was confirmed by tympanocentesis in 3332 ears. Respiratory virus infection was diagnosed during the same period in 989 patients by detecting viral antigen in nasopharyngeal mucus. There was a significant correlation between acute otitis media and respiratory virus epidemics, especially respiratory syncytial virus epidemics. There was no significant correlation between outbreaks of other respiratory viruses and acute otitis media. Acute otitis media was diagnosed in 57% of respiratory syncytial virus, 35% of influenza A virus, 33% of parainfluenza type 3 virus, 30% of adenovirus, 28% of parainfluenza type 1 virus, 18% of influenza B virus and 10% of parainfluenza type 2 virus infections. These observations show a clear association of respiratory virus infections with acute otitis media. In this study on hospitalized children Haemophilus influenzae strains were the most common bacteriologic pathogens in middle ear fluid, occurring in 19% of cases. Streptococcus pneumoniae was present in 16% and Branhamella catarrhalis in 7% of cases. There was no association between specific viruses and bacteria observed in this study.

  7. 9 CFR 113.311 - Bovine Virus Diarrhea Vaccine.

    Science.gov (United States)

    2010-01-01

    ... 9 Animals and Animal Products 1 2010-01-01 2010-01-01 false Bovine Virus Diarrhea Vaccine. 113.311... Virus Vaccines § 113.311 Bovine Virus Diarrhea Vaccine. Bovine Virus Diarrhea Vaccine shall be prepared... virus dose from the lot of Master Seed Virus shall be established as follows: (1) Twenty-five...

  8. Development of indicators for assessment of Lake Malawi Basin in an Integrated Lake Basin Management (ILBM) framework

    OpenAIRE

    Clara Limbitso Chidammodzi; Victor Shiholo Muhandiki

    2015-01-01

    This paper sought to understand the current state of the management environment of Lake Malawi Basin, deduce a lake vision and develop indicators for assessing Integrated Lake Basin Management (ILBM) in the lake basin. The premise of the study was that targeted indicators are necessary to effectively monitor the lake basin and manage it sustainably. The study focused on the Malawian side of the lake. Interviews, field observations and review of existing lake management and indicator developme...

  9. Fostering Payments for Environmental Services (PES) in the Danube Basin: Why the Danube Basin and why payments for environmental services?

    OpenAIRE

    Beckmann, A.; Gutman, P.; Long, T.

    2005-01-01

    This publication describes the characteristics of the Danube basin and reviews the history of land use and ecological health in different parts of the basin. Several studies in recent years have identified numerous threats to the basin from development, industry, agriculture, tourism, and land management practices. These threaten the fragile ecosystems that support biodiversity and provide valuable environmental services. Although Danube basin countries have implemented programs to address so...

  10. Basin Subsegments from LDEQ source data, Geographic NAD83, LOSCO (2004) [basin_subsegments_LDEQ_2004

    Data.gov (United States)

    Louisiana Geographic Information Center — This is a polygon data set of watershed basin subsegments for Louisiana. The dataset was developed for the LDEQ Office of Water Resources' watershed assessment and...

  11. Klamath Basin Restoration Agreement Off-Project Water Program Sub-basin Analysis Flow Statistics

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — Hydrological Information Products for the Off-Project Water Program of the Klamath Basin Restoration Agreement U.S. Geological Survey Open-File Report 2012-1199...

  12. The Sequence Architecture of Volcanic Basin Fillings- An Example From Xujiaweizi Faulted Depression In Songliao Basin

    Institute of Scientific and Technical Information of China (English)

    CHENG Rihui; LIU Wanzhu; SHAN Xuanlong; WANG Pujun; CHEN Shumin; QU Yongbao

    2001-01-01

    The basin filling geometric pattern of volcanic eruptive rocks depends on both of the eruptive locations in a basin and structural styles of fault terraces. It is divided into three types by eruptive locations and occurrences of eruptive rocks, including the pattern of eruption along fault and occurrence (PEAFO), the pattern of eruption on footwall of a fault and occurrence (PEOFO) and the pattern of eruption on hangingwall of fault and occurrence (PEOHO) in Xujiaweizi fault depression of Songliao basin, Northeast of China. Those basin filling patterns of volcanic eruptive rocks are of specific geometric characteristics controlling or affecting sedimentation, geometry of sedimentary body and sequence architecture during a sequence evolution. The study shows that the volcanic rocks developed at different stages of lowstand, transgressive and highstand can give different controls or affects on the sequence architecture.

  13. Klamath Basin Restoration Agreement Off-Project Water Program Sub-basin Analysis Flow Statistics

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — VERSION 5/15/2012 HYDROLOGICAL INFORMATION PRODUCTS FOR THE OFF-PROJECT WATER PROGRAM OF THE KLAMATH BASIN RESTORATION AGREEMENT By Daniel T. Snyder, John C....

  14. Autophagic machinery activated by dengue virus enhances virus replication

    International Nuclear Information System (INIS)

    Autophagy is a cellular response against stresses which include the infection of viruses and bacteria. We unravel that Dengue virus-2 (DV2) can trigger autophagic process in various infected cell lines demonstrated by GFP-LC3 dot formation and increased LC3-II formation. Autophagosome formation was also observed under the transmission electron microscope. DV2-induced autophagy further enhances the titers of extracellular and intracellular viruses indicating that autophagy can promote viral replication in the infected cells. Moreover, our data show that ATG5 protein is required to execute DV2-induced autophagy. All together, we are the first to demonstrate that DV can activate autophagic machinery that is favorable for viral replication

  15. [Bats and Viruses: complex relationships].

    Science.gov (United States)

    Rodhain, F

    2015-10-01

    With more than 1 200 species, bats and flying foxes (Order Chiroptera) constitute the most important and diverse order of Mammals after Rodents. Many species of bats are insectivorous while others are frugivorous and few of them are hematophagous. Some of these animals fly during the night, others are crepuscular or diurnal. Some fly long distances during seasonal migrations. Many species are colonial cave-dwelling, living in a rather small home range while others are relatively solitary. However, in spite of the importance of bats for terrestrial biotic communities and ecosystem ecology, the diversity in their biology and lifestyles remain poorly known and underappreciated. More than sixty viruses have been detected or isolated in bats; these animals are therefore involved in the natural cycles of many of them. This is the case, for instance, of rabies virus and other Lyssavirus (Family Rhabdoviridae), Nipah and Hendra viruses (Paramyxoviridae), Ebola and Marburg viruses (Filoviridae), SARS-CoV and MERS-CoV (Coronaviridae). For these zoonotic viruses, a number of bat species are considered as important reservoir hosts, efficient disseminators or even directly responsible of the transmission. Some of these bat-borne viruses cause highly pathogenic diseases while others are of potential significance for humans and domestic or wild animals; so, bats are an important risk in human and animal public health. Moreover, some groups of viruses developed through different phylogenetic mechanisms of coevolution between viruses and bats. The fact that most of these viral infections are asymptomatic in bats has been observed since a long time but the mechanisms of the viral persistence are not clearly understood. The various bioecology of the different bat populations allows exchange of virus between migrating and non-migrating conspecific species. For a better understanding of the role of bats in the circulation of these viral zoonoses, epidemiologists must pay attention to

  16. [Infection by hepatitis virus among the indigenous populations of South America: a review of the problem].

    Science.gov (United States)

    Echevarría, J M; Blitz-Dorfman, L; Pujot, F H

    1996-09-01

    After the report of the epidemic outbreak of delta hepatitis among the Yukpa amerindians in the early 80s, the viral hepatitis arose as an important health problem in all the Amerindian communities from the north of South America and the Amazonian Basin. Despite the few data available, the results obtained in different communities from Venezuela (Yukpa, Barí, Yanomami) have shown a high endemicity of hepatitis B and D virus infections and a significant prevalence of hepatitis E virus-specific antibody among their members. By contrast, the infection by hepatitis C virus, which is present in all the urban areas from South America, seems uncommon, or even absent among some Amerindian populations. At the moment, a satisfactory explanation for this findings has not yet been arised. However, it could be possible that the margination of these populations regarding the health care system has been keeping them free of an infection largely linked worldwide to iatrogeny. Vaccination of Amerindian populations against hepatitis B should be taken as a priority of the health care programs. Moreover, such programs should consider the iatrogenic transmission of the HCV as a matter of concern regarding such populations, since parenterally transmitted hepatitis viruses seems to spread quickly among their members once they are introduced, giving rise to serious health problems. PMID:8983357

  17. Discovery of a novel circular single-stranded DNA virus from porcine faeces.

    Science.gov (United States)

    Sikorski, Alyssa; Argüello-Astorga, Gerardo R; Dayaram, Anisha; Dobson, Renwick C J; Varsani, Arvind

    2013-01-01

    A large number of novel single-stranded DNA (ssDNA) viruses have been characterised from various environmental sources in the last 5 years. The bulk of these have been from faecal sources, and faecal sampling is an ideal non-invasive pathogen sampling method. We characterised a novel ssDNA from a porcine faecal sample from Cass Basin of the South Island of New Zealand. The novel viral genome has two large open reading frames (ORFs), which are bidirectionally transcribed and separated by intergenic regions. The largest ORF has some degree of similarity (DNA virus (PigSCV), whereas the second-largest ORF has high similarity to the putative replication-associated protein (Rep) of ChiSCV (~50 %) and bovine stool-associated circular DNA virus (BoSCV; ~30 %). Based on genome architecture, location of putative stem-loop like elements, and maximum-likelihood phylogenetic analysis of the gene encoding the Rep protein, the novel isolate belongs to the same family of ssDNA viruses as ChiSCV and BoSCV.

  18. Basins of Attraction for Chimera States

    DEFF Research Database (Denmark)

    Martens, Erik Andreas; Panaggio, Mark; Abrams, Daniel

    2016-01-01

    Chimera states---curious symmetry-broken states in systems of identical coupled oscillators---typically occur only for certain initial conditions. Here we analyze their basins of attraction in a simple system comprised of two populations. Using perturbative analysis and numerical simulation we...

  19. Stochastic basins of attraction for metastable states

    Science.gov (United States)

    Serdukova, Larissa; Zheng, Yayun; Duan, Jinqiao; Kurths, Jürgen

    2016-07-01

    Basin of attraction of a stable equilibrium point is an effective concept for stability analysis in deterministic systems; however, it does not contain information on the external perturbations that may affect it. Here we introduce the concept of stochastic basin of attraction (SBA) by incorporating a suitable probabilistic notion of basin. We define criteria for the size of the SBA based on the escape probability, which is one of the deterministic quantities that carry dynamical information and can be used to quantify dynamical behavior of the corresponding stochastic basin of attraction. SBA is an efficient tool to describe the metastable phenomena complementing the known exit time, escape probability, or relaxation time. Moreover, the geometric structure of SBA gives additional insight into the system's dynamical behavior, which is important for theoretical and practical reasons. This concept can be used not only in models with small noise intensity but also with noise whose amplitude is proportional or in general is a function of an order parameter. As an application of our main results, we analyze a three potential well system perturbed by two types of noise: Brownian motion and non-Gaussian α-stable Lévy motion. Our main conclusions are that the thermal fluctuations stabilize the metastable system with an asymmetric three-well potential but have the opposite effect for a symmetric one. For Lévy noise with larger jumps and lower jump frequencies ( α = 0.5 ) metastability is enhanced for both symmetric and asymmetric potentials.

  20. Alboran Basin, southern Spain - Part I: Geomorphology

    Energy Technology Data Exchange (ETDEWEB)

    Munoz, A. [Secretaria General de Pesca Maritima, Corazon de Maria, 8, 28002 Madrid (Spain); Ballesteros, M.; Rivera, J.; Acosta, J. [Instituto Espanol de Oceanografia, Corazon de Maria, 8, 28002 Madrid (Spain); Montoya, I. [Universidad Juan Carlos I, Campus de Mostoles, Madrid (Spain); Uchupi, E. [Woods Hole Oceanographic Institution, Woods Hole, MA 02543 (United States)

    2008-01-15

    Bathymetric, 3D relief and shaded relief maps created from multibeam echo-sounding data image the morphology of the Alboran Basin, a structural low along the east-west-trending Eurasian-African plates boundary. Topographic features in the basin are the consequence of volcanism associated with Miocene rifting, rift and post-rift sedimentation, and recent faulting resulting from the convergence of the African-Eurasian plates. Pleistiocene glacially induced regressions/transgressions when the sea level dropped to about 150 m below its present level gas seeps and bottom currents. Recent faulting and the Pleistocene transgressions/regressions led to mass-wasting, formation of turbidity currents and canyon erosion on the basin's slopes. Recent fault traces at the base of the northern basin slope have also served as passageways for thermogenic methane, the oxidation of which by bacteria led to the formation of carbonate mounds along the fault intercepts on the sea floor. Expulsion of thermogenic or biogenic gas has led to the formation of pockmarks; erosion by bottom currents has resulted in the formation of moats around seamounts and erosion of the seafloor of the Alboran Ridge and kept the southern edge of the 36 10'N high sediment free. (author)

  1. Synthetical Approach to the Romanian Tisa Basin

    OpenAIRE

    POMPEI COCEAN; ADRIANA VARTANOFF

    2007-01-01

    This issue of the Romanian Review of Regional Studies is dedicated to the synthesis of the project entitled “The analysis of the present state of territorial development tendencies in the Romanian Tisa Basin”, representing the first stage of research, at national level, of the development phenomena within the entire hydrographical basin, approached together by Romania, Hungary, Slovakia, Ukraine and Serbia.

  2. Columbia Basin College Facts & Impacts, 2002.

    Science.gov (United States)

    Columbia Basin Coll., Pasco, WA.

    This fact book for Columbia Basin College (CBC) (Washington) offers statistics on staff and faculty, students, degrees awarded, hot programs, enrollment; student services, financial aid, economic impact, educational partnerships, and governance. CBC serves more than 13,000 students annually and offers associate degrees in arts, science, and…

  3. Structure and Hydrocarbons in Junggar Basin

    Institute of Scientific and Technical Information of China (English)

    张庆春; 田在艺

    2002-01-01

    Having undergone four basin-forming tectonic cycles--Hercynian, Indosinian, Yanshanian, and Himalayan, the Junggar basin becomes a multi-cyclic superposed basin of old and hard mediun block pattern. Its multi-cyclic tectonic and sedimentary evolution results in five series of hydrocarbon source rock formations--Carboniferous, Permian, Upper Triassic, Middle-lower Jurassic, and Lower Tertiary, correspondingly forming five petroleum generation systems, of which the Permian and Middle-lower Jurassic petroleum generation systems are the most important with the highest exploration degree. Hydrocarbons are controlled by basin structure as follows: 1) Hydrocarbon accumulations are controlled by the structural styles of paleo-uplifts, paleo-overthrust belts and contorted anticline belts formed in multi-cyclic tectonic movements; 2) Important pathways for long distance lateral and vertical migration are provided respectively by unconformities and faults; 3) The pool-forming characteristics of the Permian petroleum system are controlled by paleo-structure; 4) The pool-forming characteristics of Jurassic and Tertiary petroleum system are controlled by recent structures.

  4. SEA of river basin management plans

    DEFF Research Database (Denmark)

    Larsen, Sanne Vammen; Kørnøv, Lone

    2009-01-01

    In, 2000 the European Parliament and the European Council passed the Water Framework Directive (WFD) to be implemented in all Member States. The consequence of the directive is that river basin management plans (RBMPs) shall be prepared which are legally subject to a strategic environmental...

  5. Hydrocarbon potential of intracratonic rift basins

    Energy Technology Data Exchange (ETDEWEB)

    Baker, D.G.; Derksen, S.J.

    1984-09-01

    Significant world oil reserves have been added in recent years from rift system. Examples of petroliferous rift basins may be found on nearly every major continent. As our understanding of the mechanisms of sedimentation and structure in rift basins grows, more rift systems will be found. With a few notable exceptions, rifts that have been explored in the past are those that formed along continental margins. These contain marine sediments, and the conditions of source rock, sediment type, depositional environment, and structural style are well-known exploration concepts. Intracratonic rift systems containing continental sediments, and also because of the problems perceived to accompany continental sedimentation. A good modern analog is the East African rift system. Several companies have made significant oil discoveries in different components of the Central African rift system. Average daily production for 1982 from the basins associated with the Benue trough was 107.928 BOPD. In the Abu Gabra rift component, where Marathon is currently exploring, Chevron has drilled approximately 60 wells. Nineteen of these were discoveries and tested an average rate per well of 3,500 BOPD. The Abu Gabra rift may contain up to 10 billion bbl of oil. Research indicates that this type of rift system is present in other areas of the world. Ongoing worldwide exploration has shown that intracratonic rift basins have the potential to make a significant contribution to world oil reserves.

  6. Deforestation Trends in the Congo Basin : Agriculture

    OpenAIRE

    Hourticq, Joel; Megevand, Carole; Tollens, Eric; Wehkamp, Johanna; Dulal, Hari

    2013-01-01

    The Congo Basin represents 70 percent of the African continent's forest cover and constitutes a large portion of Africa's biodiversity. Agricultural development is a central lever to help people out of poverty, as well as a key driver of deforestation. Forest-friendly agricultural development is a challenge for the region. This report describes some ways forest-friendly agricultural develo...

  7. Coping with Computer Viruses: General Discussion and Review of Symantec Anti-Virus for the Macintosh.

    Science.gov (United States)

    Primich, Tracy

    1992-01-01

    Discusses computer viruses that attack the Macintosh and describes Symantec AntiVirus for Macintosh (SAM), a commercial program designed to detect and eliminate viruses; sample screen displays are included. SAM is recommended for use in library settings as well as two public domain virus protection programs. (four references) (MES)

  8. Generation of virus like particles for epizootic hemorrhagic disease virus.

    Science.gov (United States)

    Forzan, Mario; Maan, Sushila; Mazzei, Maurizio; Belaganahalli, Manjunatha N; Bonuccelli, Lucia; Calamari, Monica; Carrozza, Maria Luisa; Cappello, Valentina; Di Luca, Mariagrazia; Bandecchi, Patrizia; Mertens, Peter P C; Tolari, Francesco

    2016-08-01

    Epizootic hemorrhagic disease virus (EHDV) is a distinct species within the genus Orbivirus, within the family Reoviridae. The epizootic hemorrhagic disease virus genome comprises ten segments of linear, double stranded (ds) RNA, which are packaged within each virus particle. The EHDV virion has a three layered capsid-structure, generated by four major viral proteins: VP2 and VP5 (outer capsid layer); VP7 (intermediate, core-surface layer) and VP3 (innermost, sub-core layer). Although EHDV infects cattle sporadically, several outbreaks have recently occurred in this species in five Mediterranean countries, indicating a potential threat to the European cattle industry. EHDV is transmitted by biting midges of the genus Culicoides, which can travel long distances through wind-born movements (particularly over water), increasing the potential for viral spread in new areas/countries. Expression systems to generate self-assembled virus like particles (VLPs) by simultaneous expression of the major capsid-proteins, have been established for several viruses (including bluetongue virus). This study has developed expression systems for production of EHDV VLPs, for use as non-infectious antigens in both vaccinology and serology studies, avoiding the risk of genetic reassortment between vaccine and field strains and facilitating large scale antigen production. Genes encoding the four major-capsid proteins of a field strain of EHDV-6, were isolated and cloned into transfer vectors, to generate two recombinant baculoviruses. The expression of these viral genes was assessed in insect cells by monitoring the presence of specific viral mRNAs and by western blotting. Electron microscopy studies confirmed the formation and purification of assembled VLPs. PMID:27473984

  9. Droplet Microfluidics for Virus Discovery

    Science.gov (United States)

    Rotem, Assaf; Cockrell, Shelley; Guo, Mira; Pipas, James; Weitz, David

    2012-02-01

    The ability to detect, isolate, and characterize an infectious agent is important for diagnosing and curing infectious diseases. Detecting new viral diseases is a challenge because the number of virus particles is often low and/or localized to a small subset of cells. Even if a new virus is detected, it is difficult to isolate it from clinical or environmental samples where multiple viruses are present each with very different properties. Isolation is crucial for whole genome sequencing because reconstructing a genome from fragments of many different genomes is practically impossible. We present a Droplet Microfluidics platform that can detect, isolate and sequence single viral genomes from complex samples containing mixtures of many viruses. We use metagenomic information about the sample of mixed viruses to select a short genomic sequence whose genome we are interested in characterizing. We then encapsulate single virions from the same sample in picoliter volume droplets and screen for successful PCR amplification of the sequence of interest. The selected drops are pooled and their contents sequenced to reconstruct the genome of interest. This method provides a general tool for detecting, isolating and sequencing genetic elements in clinical and environmental samples.

  10. Dot immunobinding assay for simultaneous detection of specific immunoglobulin G antibodies to measles virus, mumps virus, and rubella virus.

    OpenAIRE

    F. Condorelli; Ziegler, T.

    1993-01-01

    A dot immunobinding assay was used to detect antibodies to measles virus, mumps virus, and rubella virus antigens. Filter paper soaked with serum or whole blood was directly applied to the antigen-coated nitrocellulose sheets. The test was easy to perform, and its results agreed very well with those obtained by standard enzyme immunoassay.

  11. Social Learning in European River-Basin Management: Barriers and Fostering Mechanisms from 10 River Basins

    OpenAIRE

    Brad Searle; David Tàbara; Yvonne Rees; Claudia Pahl-Wostl; Erik Mostert; Joanne Tippett

    2007-01-01

    We present and analyze 10 case studies of participatory river-basin management that were conducted as part of the European HarmoniCOP project. The main theme was social learning, which emphasizes the importance of collaboration, organization, and learning. The case studies show that social learning in river-basin management is not an unrealistic ideal. Resistance to social learning was encountered, but many instances of social learning were found, and several positive results were identified....

  12. Basin-wide water accounting based on remote sensing data: an application for the Indus Basin

    OpenAIRE

    P. Karimi; W. G. M. Bastiaanssen; Molden, D.; M. J. M. Cheema

    2013-01-01

    The paper demonstrates the application of a new water accounting plus (WA+) framework to produce information on depletion of water resources, storage change, and land and water productivity in the Indus basin. It shows how satellite-derived estimates of land use, rainfall, evaporation (E), transpiration (T), interception (I) and biomass production can be used in addition to measured basin outflow, for water accounting with WA+. It is demonstrated how the accounting results can be interpreted ...

  13. Messinian paleoenvironmental changes in the easternmost Mediterranean Basin: Adana Basin, southern Turkey

    OpenAIRE

    FARANDA, Costanza; Gliozzi, Elsa; CIPOLLARI, Paola; Grossi, Francesco; Darbaş, Güldemin

    2013-01-01

    We present detailed Messinian paleoenvironmental reconstructions for the Adana Basin based on a multidisciplinary approach that utilizes the fossil content of the Kuzgun and Handere Formations. To reconstruct the paleoenvironmental changes that affected the Adana Basin during the Messinian, we analyzed mollusk, ostracod, planktonic and benthonic foraminifer, and calcareous nannofossil assemblages from 2 stratigraphic sections near Kabasakal village (Adana, southern Turkey). To determine if th...

  14. Predictive Modeling of West Nile Virus Transmission Risk in the Mediterranean Basin: How Far from Landing?

    OpenAIRE

    Véronique Chevalier; Annelise Tran; Benoit Durand

    2013-01-01

    The impact on human and horse health of West Nile fever (WNF) recently and dramatically increased in Europe and neighboring countries. Involving several mosquito and wild bird species, WNF epidemiology is complex. Despite the implementation of surveillance systems in several countries of concern, and due to a lack of knowledge, outbreak occurrence remains unpredictable. Statistical models may help identifying transmission risk factors. When spatialized, they provide tools to identify areas th...

  15. Modeling Nitrogen Losses under Rapid Infiltration Basins

    Science.gov (United States)

    Akhavan, M.; Imhoff, P. T.; Andres, A. S.; Finsterle, S.

    2011-12-01

    Rapid Infiltration Basin System (RIBS) is one of the major land treatment techniques used for wastewater treatment and reuse of recovered treated wastewater. In this system, wastewater that is treated using primary, secondary, or advanced treatment techniques is applied at high rates to shallow basins constructed in permeable deposits of soil or sand, with further treatment occurring in soil and the vadose zone before the water recharges groundwater. Because the influent wastewater is usually enriched in nitrogen (N) compounds, there is particular concern that RIBS may contaminant groundwater or nearby surface waters if not designed and operated properly. In most of the new sequenced batch reactor (SBR) wastewater treatment plants, N is found in the form of nitrate in the discharged wastewater, so denitrification (DNF) is the main reaction in N removal. The absence of molecular oxygen is one of the required conditions for DNF. During RIBS operation, application of wastewater is cyclic and typically consists of a flooding period followed by days or weeks of drying. Key operational parameters include the ratio of wetting to drying time and the hydraulic loading rate, which affect water saturation and air content in the vadose zone and as a result have an impact on DNF. Wastewater is typically distributed at a limited number of discharge points in RIBS and basins are not usually completely flooded which result in non-homogeneous distribution of wastewater and unusual surface water flow patterns. For this reason, we couple overland flow within RIBS with subsurface flow to investigate the influence of non-uniform application of wastewater on DNF. No modeling effort has been done for understanding this aspect of RIBS performance previously. TOUGH2/ iTOUGH2, a general-purpose numerical simulation program for multi-phase fluid flow in porous media, is used for modeling fluid movement. Water saturation is used as a surrogate parameter to evaluate oxygen limitations in the

  16. A Literature Review of Zika Virus.

    Science.gov (United States)

    Plourde, Anna R; Bloch, Evan M

    2016-07-01

    Zika virus is a mosquitoborne flavivirus that is the focus of an ongoing pandemic and public health emergency. Previously limited to sporadic cases in Africa and Asia, the emergence of Zika virus in Brazil in 2015 heralded rapid spread throughout the Americas. Although most Zika virus infections are characterized by subclinical or mild influenza-like illness, severe manifestations have been described, including Guillain-Barre syndrome in adults and microcephaly in babies born to infected mothers. Neither an effective treatment nor a vaccine is available for Zika virus; therefore, the public health response primarily focuses on preventing infection, particularly in pregnant women. Despite growing knowledge about this virus, questions remain regarding the virus's vectors and reservoirs, pathogenesis, genetic diversity, and potential synergistic effects of co-infection with other circulating viruses. These questions highlight the need for research to optimize surveillance, patient management, and public health intervention in the current Zika virus epidemic. PMID:27070380

  17. Virus Discovery Using Tick Cell Lines

    Science.gov (United States)

    Bell-Sakyi, Lesley; Attoui, Houssam

    2016-01-01

    While ticks have been known to harbor and transmit pathogenic arboviruses for over 80 years, the application of high-throughput sequencing technologies has revealed that ticks also appear to harbor a diverse range of endogenous tick-only viruses belonging to many different families. Almost nothing is known about these viruses; indeed, it is unclear in most cases whether the identified viral sequences are derived from actual replication-competent viruses or from endogenous virus elements incorporated into the ticks’ genomes. Tick cell lines play an important role in virus discovery and isolation through the identification of novel viruses chronically infecting such cell lines and by acting as host cells to aid in determining whether or not an entire replication-competent, infective virus is present in a sample. Here, we review recent progress in tick-borne virus discovery and comment on the actual and potential applications for tick cell lines in this emerging research area. PMID:27679414

  18. Bats and Viruses: a Brief Review

    Institute of Scientific and Technical Information of China (English)

    Lin-Fa Wang

    2009-01-01

    Bats, probably the most abundant, diverse and geographically dispersed vertebrates on earth, have recently been shown to be the reservoir hosts of a number of emerging viruses responsible for severe human and livestock disease outbreaks. Flying foxes have been demonstrated to be the natural reservoir for Hendra and Nipah viruses. Evidence supporting the possibility of bats as potential reservoirs for SARS coronavirus (SARS-CoV) and Ebola virus has also been reported. The recent discovery of these viruses and other viruses occurring naturally in the bat population provides a unique insight into a diverse pool of potentially emergent and pathogenic viruses. The factors which influence the ability of zoonotic viruses to effectively cross the species barrier from bats to other animal populations are poorly understood. A brief review is provided here on the recently emerged bat viruses and on current and future strategies for research in this area.

  19. West Nile Virus Cases, 2006-present

    Data.gov (United States)

    U.S. Department of Health & Human Services — This dataset contains positive cases of West Nile virus found in humans by county of residence, 2006-present. Humans usually become infected with West Nile virus by...

  20. A Literature Review of Zika Virus.

    Science.gov (United States)

    Plourde, Anna R; Bloch, Evan M

    2016-07-01

    Zika virus is a mosquitoborne flavivirus that is the focus of an ongoing pandemic and public health emergency. Previously limited to sporadic cases in Africa and Asia, the emergence of Zika virus in Brazil in 2015 heralded rapid spread throughout the Americas. Although most Zika virus infections are characterized by subclinical or mild influenza-like illness, severe manifestations have been described, including Guillain-Barre syndrome in adults and microcephaly in babies born to infected mothers. Neither an effective treatment nor a vaccine is available for Zika virus; therefore, the public health response primarily focuses on preventing infection, particularly in pregnant women. Despite growing knowledge about this virus, questions remain regarding the virus's vectors and reservoirs, pathogenesis, genetic diversity, and potential synergistic effects of co-infection with other circulating viruses. These questions highlight the need for research to optimize surveillance, patient management, and public health intervention in the current Zika virus epidemic.

  1. Nucleocytoplasmic Shuttling of Influenza A Virus Proteins

    Directory of Open Access Journals (Sweden)

    Jing Li

    2015-05-01

    Full Text Available Influenza viruses transcribe and replicate their genomes in the nuclei of infected host cells. The viral ribonucleoprotein (vRNP complex of influenza virus is the essential genetic unit of the virus. The viral proteins play important roles in multiple processes, including virus structural maintenance, mediating nucleocytoplasmic shuttling of the vRNP complex, virus particle assembly, and budding. Nucleocytoplasmic shuttling of viral proteins occurs throughout the entire virus life cycle. This review mainly focuses on matrix protein (M1, nucleoprotein (NP, nonstructural protein (NS1, and nuclear export protein (NEP, summarizing the mechanisms of their nucleocytoplasmic shuttling and the regulation of virus replication through their phosphorylation to further understand the regulation of nucleocytoplasmic shuttling in host adaptation of the viruses.

  2. Influenza Type A Viruses and Subtypes

    Science.gov (United States)

    ... Research Making a Candidate Vaccine Virus Related Links Influenza Types Seasonal Avian Swine Variant Pandemic Other Get ... this? Submit What's this? Submit Button Past Newsletters Influenza Type A Viruses Language: English Español Recommend ...

  3. Duck Virus Enteritis - A Contingency Plan

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — Duck plague, also known as duck virus enteritis (DVE) is a highly contagious, extremely deadly epizootic virus with a potential for devastating continental...

  4. Evolution of Mesozoic Volcanic Basins and Red Basins in the Gan-Hang Tectonic-Volcanic Metallogenic Belt

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    This paper mainly proposes six major regional geological events in the active continental-margin mantle uplift zone and discusses the oscillation nature of the evolution of Mesozoic volcanic basins and red basins, origin of erosion in the late stage of red basins and mechanism of volcanism.

  5. Area environmental characterization report of the Dalhart and Palo Duro basins in the Texas Panhandle. Volume I. Dalhart Basin

    International Nuclear Information System (INIS)

    This area report describes the environmental characteristics of the Dalhart and Palo Duro basins of the Texas Panhandle portion of the Permian basin. Both basins are rather sparsely populated, and the overall population is decreasing. The economic base is centered on agribusiness and manufacturing. Most of the potentially conflicting land uses in both basins (i.e., parks, historic sites) occupy small land areas, with the exception of a national grassland in the Dalhart and military air training routes in both basins. Ground transportation in the Dalhart basin is adequate, and it is well developed in the Palo Duro basin. In both basins irrigation constitutes the principal water use, and groundwater is the principal source. However, the dominant aquifer, the Ogallala, is being depleted. Both basins consist primarily of grasslands, rangelands, and agricultural areas. No critical terrestrial or aquatic habitats have been identified in the basins, though several endangered, threatened, or rare terrestrial species occur in or near the basins. Aquatic resources in both basins are limited because of the intermittent availability of water and the high salt content of some water bodies. Playa lakes are common, though usually seasonal or rain dependent. The climate of the area is semiarid, with low humidity, relatively high wind speeds, and highly variable prcipitation. Restrictive dispersion conditions are infrequent. National ambient secondary air quality standards for particulates are being exceeded in the area, largely because of fugitive dust, although there are some particulate point sources

  6. Messinian Salinity Crisis and basin fluid flow

    Science.gov (United States)

    Bertoni, Claudia; Cartwight, Joe

    2014-05-01

    Syn- and post-depositional movement of fluids through sediments is one of the least understood aspects in the evolution of a basin. The conventional hydrostratigraphic view on marine sedimentary basins assumes that compactional and meteoric groundwater fluid circulation drives fluid movement and defines its timing. However, in the past few years, several examples of instantaneous and catastrophic release of fluids have been observed even through low-permeability sediments. A particularly complex case-study involves the presence of giant salt bodies in the depocentres of marine basins. Evaporites dramatically change the hydrostratigraphy and fluid-dynamics of the basin, and influence the P/T regimes, e.g. through changes in the geothermal gradient and in the compaction of underlying sediments. Our paper reviews the impact of the Messinian Salinity Crisis (MSC) and evaporites on fluid flow in the Mediterranean sub-basins. The analysis of geological and geophysical sub-surface data provides examples from this basin, and the comparison with analogues in other well-known evaporitic provinces. During the MSC, massive sea-level changes occurred in a relatively limited time interval, and affected the balance of fluid dynamics, e.g. with sudden release or unusual trapping of fluids. Fluid expulsion events are here analysed and classified in relation to the long and short-term effects of the MSC. Our main aim is to build a framework for the correct identification of the fluid flow-related events, and their genetic mechanisms. On basin margins, where evaporites are thin or absent, the sea-level changes associated with the MSC force a rapid basinward shift of the mixing zone of meteoric/gravity flow and saline/compactional flow, 100s-km away from its pre-MSC position. This phenomenon changes the geometry of converging flows, creates hydraulic traps for fluids, and triggers specific diagenetic reactions in pre-MSC deep marine sediments. In basin-centre settings, unloading and

  7. Classification of Complex Reservoirs in Superimposed Basins of Western China

    Institute of Scientific and Technical Information of China (English)

    PANG Xiongqi; ZHOU Xinyuan; LIN Changsong; HUO Zhipeng; LUO Xiaorong; PANG Hong

    2010-01-01

    Many of the sedimentary basins in western China were formed through the superposition and compounding of at least two previously developed sedimentary basins and in general they can be termed as complex superimposed basins.The distinct differences between these basins and monotype basins are their discontinuous stratigraphic sedimentation,stratigraphic structure and stratigraphic stress-strain action over geological history.Based on the correlation of chronological age on structural sections,superimposed basins can be divided into five types in this study:(1)continuous sedimentation type superimposed basins,(2)middle and late stratigraphic superimposed basins,(3)early and late stratigraphic superimposed basins,(4)early and middle stratigraphic superimposed basins,and(5)long-term exposed superimposed basins.Multiple source-reservoir-caprock assemblages have developed in such basins.In addition,multi-stage hydrocarbon generation and expulsion,multiple sources,polycyclic hydrocarbon accumulation and multiple-type hydrocarbon reservoirs adjustment,reformation and destruction have occurred in these basins.The complex reservoirs that have been discovered widely in the superimposed basins to date have remarkably different geologic features from primary reservoirs,and the root causes of this are folding,denudation and the fracture effect caused by multiphase tectonic events in the superimposed basins as well as associated seepage,diffusion,spilling,oxidation,degradation and cracking.Based on their genesis characteristics,complex reservoirs are divided into five categories:(1)primary reservoirs,(2)trap adjustment type reservoirs,(3)component variant reservoirs,(4)phase conversion type reservoirs and(5)scale-reformed reservoirs.

  8. General properties of grapevine viruses occurring in Hungary

    Directory of Open Access Journals (Sweden)

    Eszter Cseh

    2012-03-01

    Full Text Available The past fifty years important advances have been made in the field of grapevine virus research, including characterization of pathogens and control measurements. Still the occurrence of Grapevine fanleaf virus (GFLV, Arabis mosaic virus (ArMV, Tomato black ring virus (TBRV, Grapevine chrome mosaic virus (GCMV, Alfalfa mosaic virus (AMV, Grapevine Bulgarian latent virus (GBLV, Grapevine fleck virus (GFkV, Grapevine leafroll- associated viruses (GLRaV1-4, Grapevine virus A (GVA, Grapevine virus B (GVB and Grapevine rupestris stem pitting- associated virus (GRSPaV have been reported in Hungary and characterized by conventional methods as woody indexing, herbaceous indexing and serological methods. Among grapevine viruses the Grapevine line pattern virus (GLPV seems to be uncial; because it was reported only in Hungary. Causal agents of several grapevine diseases, like enation, vein necrosis and vein mosaic remained undiscovered. These virus-like diseases occurred only sporadically, without economic importance.

  9. Analysis of K west basin canister gas

    Energy Technology Data Exchange (ETDEWEB)

    Trimble, D.J., Fluor Daniel Hanford

    1997-03-06

    Gas and Liquid samples have been collected from a selection of the approximately 3,820 spent fuel storage canisters in the K West Basin. The samples were taken to characterize the contents of the gas and water in the canisters providing source term information for two subprojects of the Spent Nuclear Fuel Project (SNFP) (Fulton 1994): the K Basins Integrated Water Treatment System Subproject (Ball 1996) and the K Basins Fuel Retrieval System Subproject (Waymire 1996). The barrels of ten canisters were sampled for gas and liquid in 1995, and 50 canisters were sampled in a second campaign in 1996. The analysis results from the first campaign have been reported (Trimble 1995a, 1995b, 1996a, 1996b). The analysis results from the second campaign liquid samples have been documented (Trimble and Welsh 1997; Trimble 1997). This report documents the results for the gas samples from the second campaign and evaluates all gas data in terms of expected releases when opening the canisters for SNFP activities. The fuel storage canisters consist of two closed and sealed barrels, each with a gas trap. The barrels are attached at a trunion to make a canister, but are otherwise independent (Figure 1). Each barrel contains up to seven N Reactor fuel element assemblies. A gas space of nitrogen was established in the top 2.2 to 2.5 inches (5.6 to 6.4 cm) of each barrel. Many of the fuel elements were damaged allowing the metallic uranium fuel to be corroded by the canister water. The corrosion releases fission products and generates hydrogen gas. The released gas mixes with the gas-space gas and excess gas passes through the gas trap into the basin water. The canister design does not allow canister water to be exchanged with basin water.

  10. Magmatism in rifting and basin formation

    Science.gov (United States)

    Thybo, H.

    2008-12-01

    Whether heating and magmatism cause rifting or rifting processes cause magmatic activity is highly debated. The stretching factor in rift zones can be estimated as the relation between the initial and the final crustal thickness provided that the magmatic addition to the crust is insignificant. Recent research demonstrates substantial magmatic intrusion into the crust in the form of sill like structures in the lowest crust in the presently active Kenya and Baikal rift zones and the DonBas palaeo-rift zone in Ukraine. This result may be surprising as the Kenya Rift is associated with large amounts of volcanic products, whereas the Baikal Rift shows very little volcanism. Identification of large amounts of magmatic intrusion into the crust has strong implications for estimation of stretching factor, which in the case of Baikal Rift Zone is around 1.7 but direct estimation gives a value of 1.3-1.4 if the magmatic addition is not taken into account. This may indicate that much more stretching has taken place on rift systems than hitherto believed. Wide sedimentary basins may form around aborted rifts due to loading of the lithosphere by sedimentary and volcanic in-fill of the rift. This type of subsidence will create wide basins without faulting. The Norwegian- Danish basin in the North Sea area also has subsided gradually during the Triassic without faulting, but only few rift structures have been identified below the Triassic sequences. We have identified several mafic intrusions in the form of large batholiths, typically more than 100 km long, 20-40 km wide and 20 km thick. The associated heating would have lifted the surface by about 2 km, which may have been eroded before cooling. The subsequent contraction due to solidification and cooling would create subsidence in a geometry similar to basins that developed by loading. These new aspects of magmatism will be discussed with regard to rifting and basin formation.

  11. Water Temperature Controls in Arctic Basins

    Science.gov (United States)

    Neilson, B. T.; King, T.; Schmadel, N. M.; Heavilin, J.; Overbeck, L. D.; Kane, D. L.

    2015-12-01

    Understanding the dynamics of heat transfer mechanisms in arctic rivers is critical for forecasting the effects of climate change on river temperatures. Building on the collection of key data and a dynamic river temperature model that accounts for heat fluxes found important in temperate climates, we were able to identify portions of an arctic basin and hydrologic conditions for which heat flux dynamics differ from those found in temperate systems. During the open water season, similarities in heat flux influences include dominant shortwave radiation, greater surface exchanges than bed exchanges and greater influences of lateral inflows in the lower order portions of the basin. Differing from temperate systems, the heat flux contribution of net longwave radiation is consistently negative and both latent heat and bed friction are negligible. Despite these differences, accounting for the bulk lateral inflows from the basin resulted in accurate predictions during higher flows. Under lower flow conditions, however, lateral inflows were limited and resulting temperature predictions were poor. Work in a temperate system demonstrated that spatial variability in hydraulics influencing stream residence times are necessary for accurate river temperature predictions. Because heat fluxes at the air-water interface become increasingly dominant at low flows and these fluxes are sensitive to parameters representing the water surface area to volume ratio, similar to temperate systems, we expect that high-resolution representations of stream geometry and hydraulics are important both for accurate flux and residence time estimates. Furthermore, given the highly dynamic nature of flows in arctic basins, we anticipate that detailed information regarding spatially variable hydraulic characteristics (e.g., channel width, depth, and velocity) is critical for accurate predictions in low arctic rivers through a large range of flow conditions. Upon identifying key processes controlling

  12. Characteristics of Filoviridae: Marburg and Ebola Viruses

    Science.gov (United States)

    Beer, Brigitte; Kurth, Reinhard; Bukreyev, Alexander

    Filoviruses are enveloped, nonsegmented negative-stranded RNA viruses. The two species, Marburg and Ebola virus, are serologically, biochemically, and genetically distinct. Marburg virus was first isolated during an outbreak in Europe in 1967, and Ebola virus emerged in 1976 as the causative agent of two simultaneous outbreaks in southern Sudan and northern Zaire. Although the main route of infection is known to be person-to-person transmission by intimate contact, the natural reservoir for filoviruses still remains a mystery.

  13. Citrus tristeza virus-host interactions

    OpenAIRE

    Dawson, William O.; Garnsey, Stephen M.; Satyanarayana eTatineni; Folimonova, Svetlana Y.; Scott J Harper; S eGowda

    2013-01-01

    Citrus tristeza virus (CTV) is a phloem-limited virus whose natural host range is restricted to citrus and related species. Although the virus has killed millions of trees, almost destroying whole industries, and continually limits production in many citrus growing areas, most isolates are mild or symptomless in most of their host range. There is little understanding of how the virus causes severe disease in some citrus and none in others. Movement and distribution of CTV differs considerably...

  14. Viruses and Sialic Acids: Rules of Engagement

    OpenAIRE

    Neu, Ursula; Bauer, Johannes; Stehle, Thilo

    2011-01-01

    Viral infections are initiated by specific attachment of a virus particle to receptors at the surface of the host cell. For many viruses, these receptors are glycans that are linked to either a protein or a lipid. Glycans terminating in sialic acid and its derivatives serve as receptors for a large number of viruses, including several human pathogens. In combination with glycan array analyses, structural analyses of complexes of viruses with sialylated oligosaccharides have provided insights ...

  15. RNA virus genomics: a world of possibilities

    OpenAIRE

    Edward C Holmes

    2009-01-01

    The increasing availability of complete genome sequences of RNA viruses has the potential to shed new light on fundamental aspects of their biology. Here, I use case studies of 3 RNA viruses to explore the impact of genomic sequence data, with particular emphasis on influenza A virus. Notably, the studies of RNA virus genomics undertaken to date largely focused on issues of evolution and epidemiology, and they have given these disciplines new impetus. However, genomic data have so far made fe...

  16. Viruses and prions of Saccharomyces cerevisiae

    OpenAIRE

    Wickner, Reed B.; Fujimura, Tsutomu; Esteban, Rosa

    2013-01-01

    Saccharomyces cerevisiae has been a key experimental organism for the study of infectious diseases, including dsRNA viruses, ssRNA viruses, and prions. Studies of the mechanisms of virus and prion replication, virus structure, and structure of the amyloid filaments that are the basis of yeast prions have been at the forefront of such studies in these classes of infectious entities. Yeast has been particularly useful in defining the interactions of the infectious elements with cellular compone...

  17. Novel hemagglutinin-based influenza virus inhibitors

    OpenAIRE

    Shen, Xintian; Zhang, Xuanxuan; Liu, Shuwen

    2013-01-01

    Influenza virus has caused seasonal epidemics and worldwide pandemics, which caused tremendous loss of human lives and socioeconomics. Nowadays, only two classes of anti-influenza drugs, M2 ion channel inhibitors and neuraminidase inhibitors respectively, are used for prophylaxis and treatment of influenza virus infection. Unfortunately, influenza virus strains resistant to one or all of those drugs emerge frequently. Hemagglutinin (HA), the glycoprotein in influenza virus envelope, plays a c...

  18. Unusual Influenza A Viruses in Bats

    OpenAIRE

    Andrew Mehle

    2014-01-01

    Influenza A viruses infect a remarkably diverse number of hosts. Two completely new influenza A virus subtypes were recently discovered in bats, dramatically expanding the host range of the virus. These bat viruses are extremely divergent from all other known strains and likely have unique replication cycles. Phylogenetic analysis indicates long-term, isolated evolution in bats. This is supported by a high seroprevalence in sampled bat populations. As bats represent ~20% of all classified mam...

  19. Petroleum systems of the Taoudeni Basin,West Africa

    Institute of Scientific and Technical Information of China (English)

    Huang Zhilong; Zhao Baoshun; Jiang Qingchun; Wang Songpo; Liu Bo

    2008-01-01

    The Taoudeni Basin is a typical steady intracratonic basin. Based on the distribution of effective source rocks in the Taoudeni Basin, combined with the structure characteristics of the basin and the distribution characteristics of reservoir beds, two petroleum systems are recognized in the basin:the infra-Cambrian petroleum system and the Silurian petroleum system. Structural uplift and timing of petroleum generation controlled the timing of petroleum charging and preservation of hydrocarbon accumulations. Maturity, evolution history, and distribution of effective source rocks controlled hydrocarbon richness. The geological key factors and geological processes controlled the type of hydrocarbon accumulations.

  20. Numerical modelling of ground motion in the Taipei Basin: basin and source effects

    Science.gov (United States)

    Miksat, J.; Wen, K.-L.; Wenzel, F.; Sokolov, V.; Chen, C.-T.

    2010-12-01

    The Taipei basin in northern Taiwan is located in a high seismicity region and was affected by several earthquakes in the past (ML = 7.3 on 1909 April 15; ML = 6.8 on 1986 November 15; the Chi-Chi ML = 7.3 earthquake on 1999 September 21 and ML = 6.8 on 2002 March 31). The main characteristic of the Taipei basin is its complex shape with a deep western and shallow eastern part. The uppermost Sungshan formation with its low shear wave velocities (90-200ms-1) is also a distinct feature of the basin. Based on the large data base of earthquake records obtained from the Taiwan Strong Motion Instrumentation Program network, many studies on ground motion within the Taipei basin exist. However, the influence of the various subsurface structures on the observed ground motions as well as the variability of ground motion with respect to earthquake location is not fully understood. We apply a 3-D finite-difference method to simulate wave propagation up to 1Hz for a small earthquake close to the basin in order to resolve these open questions. By varying source and structural parameters, we explore the variability of ground motion. Our study includes a subsurface model that is based on recent studies on the basin structure and on the crustal structure of Taiwan. From our simulations we find a good fit between simulated and observed waveforms and peak ground accelerations for the considered small earthquake near the basin. We also explore the influence of fault plane orientation, hypocentre location, deep basin structure and soft soil surface layers of the Sungshan formation by varying the subsurface structure and earthquake position. Our studies reveal that the basin structure produces an amplification factor of about 4 compared to hard rock conditions. Additionally, the soft soil Sungshan formation produce amplification of a factor of 2. This results in a maximum amplification of the basin structure of about 8, which is in good comparison with amplification values larger than 5

  1. Hepatitis Virus Infections in Poultry.

    Science.gov (United States)

    Yugo, Danielle M; Hauck, Ruediger; Shivaprasad, H L; Meng, Xiang-Jin

    2016-09-01

    Viral hepatitis in poultry is a complex disease syndrome caused by several viruses belonging to different families including avian hepatitis E virus (HEV), duck hepatitis B virus (DHBV), duck hepatitis A virus (DHAV-1, -2, -3), duck hepatitis virus Types 2 and 3, fowl adenoviruses (FAdV), and turkey hepatitis virus (THV). While these hepatitis viruses share the same target organ, the liver, they each possess unique clinical and biological features. In this article, we aim to review the common and unique features of major poultry hepatitis viruses in an effort to identify the knowledge gaps and aid the prevention and control of poultry viral hepatitis. Avian HEV is an Orthohepevirus B in the family Hepeviridae that naturally infects chickens and consists of three distinct genotypes worldwide. Avian HEV is associated with hepatitis-splenomegaly syndrome or big liver and spleen disease in chickens, although the majority of the infected birds are subclinical. Avihepadnaviruses in the family of Hepadnaviridae have been isolated from ducks, snow geese, white storks, grey herons, cranes, and parrots. DHBV evolved with the host as a noncytopathic form without clinical signs and rarely progressed to chronicity. The outcome for DHBV infection varies by the host's ability to elicit an immune response and is dose and age dependent in ducks, thus mimicking the pathogenesis of human hepatitis B virus (HBV) infections and providing an excellent animal model for human HBV. DHAV is a picornavirus that causes a highly contagious virus infection in ducks with up to 100% flock mortality in ducklings under 6 wk of age, while older birds remain unaffected. The high morbidity and mortality has an economic impact on intensive duck production farming. Duck hepatitis virus Types 2 and 3 are astroviruses in the family of Astroviridae with similarity phylogenetically to turkey astroviruses, implicating the potential for cross-species infections between strains. Duck astrovirus (DAstV) causes

  2. [Zika virus, an emerging threat].

    Science.gov (United States)

    Salinas, Sara; Foulongne, Vincent; Loustalot, Fabien; Fournier-Wirth, Chantal; Molès, Jean-Pierre; Briant, Laurence; Nagot, Nicolas; Van de Perre, Philippe; Simonin, Yannick

    2016-04-01

    Zika virus, discovered in 1947, is particularly publicized because of its involvement in a major epidemic that began in 2015 and which epicenter is located in Latin America, mainly in Brazil. In the majority of cases (70-80 %) the infection is asymptomatic, however in some patients, moderate fever, skin rash, conjunctivitis and myalgia may occur. More alarming, neurological complications are reported, in particular cases of microcephaly probably resulting from the infection of women in the first or second trimester of pregnancy. Moreover, Guillain-Barré syndromes have also been identified in patients whose infection was confirmed. The extent of the current outbreak reveals the very primitive state of knowledge about the pathophysiology of this virus. Thus, a global effort is being undertaken in order to quickly characterize the molecular interaction of the virus with human cells, but also to develop specific diagnostic assays and vaccinal approaches. PMID:27137695

  3. [Zika virus, an emerging threat].

    Science.gov (United States)

    Salinas, Sara; Foulongne, Vincent; Loustalot, Fabien; Fournier-Wirth, Chantal; Molès, Jean-Pierre; Briant, Laurence; Nagot, Nicolas; Van de Perre, Philippe; Simonin, Yannick

    2016-04-01

    Zika virus, discovered in 1947, is particularly publicized because of its involvement in a major epidemic that began in 2015 and which epicenter is located in Latin America, mainly in Brazil. In the majority of cases (70-80 %) the infection is asymptomatic, however in some patients, moderate fever, skin rash, conjunctivitis and myalgia may occur. More alarming, neurological complications are reported, in particular cases of microcephaly probably resulting from the infection of women in the first or second trimester of pregnancy. Moreover, Guillain-Barré syndromes have also been identified in patients whose infection was confirmed. The extent of the current outbreak reveals the very primitive state of knowledge about the pathophysiology of this virus. Thus, a global effort is being undertaken in order to quickly characterize the molecular interaction of the virus with human cells, but also to develop specific diagnostic assays and vaccinal approaches.

  4. Virus Dynamics on Starlike Graphs

    CERN Document Server

    Becker, Thealexa; Kontorovich, Leonid Aryeh; Miller, Steven J; Ravikumar, Pradeep; Shen, Karen

    2011-01-01

    The field of epidemiology has presented fascinating and relevant questions for mathematicians, primarily concerning the spread of viruses in a community. The importance of this research has greatly increased over time as its applications have expanded to also include studies of electronic and social networks and the spread of information and ideas. We study virus propagation on a non-linear hub and spoke graph (which models well many airline networks). We determine the long-term behavior as a function of the cure and infection rates, as well as the number of spokes n. For each n we prove the existence of a critical threshold relating the two rates. Below this threshold, the virus always dies out; above this threshold, all non-trivial initial conditions iterate to a unique non-trivial steady state. We end with some generalizations to other networks.

  5. Ebola Virus Persistence in Semen Ex Vivo.

    Science.gov (United States)

    Fischer, Robert J; Judson, Seth; Miazgowicz, Kerri; Bushmaker, Trent; Munster, Vincent J

    2016-02-01

    On March 20, 2015, a case of Ebola virus disease was identified in Liberia that most likely was transmitted through sexual contact. We assessed the efficiency of detecting Ebola virus in semen samples by molecular diagnostics and the stability of Ebola virus in ex vivo semen under simulated tropical conditions.

  6. Ebola Virus Persistence in Semen Ex Vivo

    OpenAIRE

    Fischer, Robert J.; Judson, Seth; Miazgowicz, Kerri; Bushmaker, Trent; Munster, Vincent J.

    2016-01-01

    On March 20, 2015, a case of Ebola virus disease was identified in Liberia that most likely was transmitted through sexual contact. We assessed the efficiency of detecting Ebola virus in semen samples by molecular diagnostics and the stability of Ebola virus in ex vivo semen under simulated tropical conditions.

  7. Nipah virus transmission in a hamster model.

    Directory of Open Access Journals (Sweden)

    Emmie de Wit

    2011-12-01

    Full Text Available Based on epidemiological data, it is believed that human-to-human transmission plays an important role in Nipah virus outbreaks. No experimental data are currently available on the potential routes of human-to-human transmission of Nipah virus. In a first dose-finding experiment in Syrian hamsters, it was shown that Nipah virus was predominantly shed via the respiratory tract within nasal and oropharyngeal secretions. Although Nipah viral RNA was detected in urogenital and rectal swabs, no infectious virus was recovered from these samples, suggesting no viable virus was shed via these routes. In addition, hamsters inoculated with high doses shed significantly higher amounts of viable Nipah virus particles in comparison with hamsters infected with lower inoculum doses. Using the highest inoculum dose, three potential routes of Nipah virus transmission were investigated in the hamster model: transmission via fomites, transmission via direct contact and transmission via aerosols. It was demonstrated that Nipah virus is transmitted efficiently via direct contact and inefficiently via fomites, but not via aerosols. These findings are in line with epidemiological data which suggest that direct contact with nasal and oropharyngeal secretions of Nipah virus infected individuals resulted in greater risk of Nipah virus infection. The data provide new and much-needed insights into the modes and efficiency of Nipah virus transmission and have important public health implications with regards to the risk assessment and management of future Nipah virus outbreaks.

  8. Virus infections of honeybees Apis Mellifera

    Directory of Open Access Journals (Sweden)

    Giuseppina Tantillo

    2015-09-01

    Full Text Available The health and vigour of honeybee colonies are threatened by numerous parasites (such as Varroa destructor and Nosema spp. and pathogens, including viruses, bacteria, protozoa. Among honeybee pathogens, viruses are one of the major threats to the health and wellbeing of honeybees and cause serious concern for researchers and beekeepers. To tone down the threats posed by these invasive organisms, a better understanding of bee viral infections will be of crucial importance in developing effective and environmentally benign disease control strategies. Here we summarize recent progress in the understanding of the morphology, genome organization, transmission, epidemiology and pathogenesis of eight honeybee viruses: Deformed wing virus (DWV and Kakugo virus (KV; Sacbrood virus (SBV; Black Queen cell virus (BQCV; Acute bee paralysis virus (ABPV; Kashmir bee virus (KBV; Israeli Acute Paralysis Virus (IAPV; Chronic bee paralysis virus (CBPV. The review has been designed to provide researchers in the field with updated information about honeybee viruses and to serve as a starting point for future research.

  9. Virus Infections of Honeybees Apis Mellifera

    Science.gov (United States)

    Tantillo, Giuseppina; Bottaro, Marilisa; Di Pinto, Angela; Martella, Vito; Di Pinto, Pietro

    2015-01-01

    The health and vigour of honeybee colonies are threatened by numerous parasites (such as Varroa destructor and Nosema spp.) and pathogens, including viruses, bacteria, protozoa. Among honeybee pathogens, viruses are one of the major threats to the health and well-being of honeybees and cause serious concern for researchers and beekeepers. To tone down the threats posed by these invasive organisms, a better understanding of bee viral infections will be of crucial importance in developing effective and environmentally benign disease control strategies. Here we summarize recent progress in the understanding of the morphology, genome organization, transmission, epidemiology and pathogenesis of eight honeybee viruses: Deformed wing virus (DWV) and Kakugo virus (KV); Sacbrood virus (SBV); Black Queen cell virus (BQCV); Acute bee paralysis virus (ABPV); Kashmir bee virus (KBV); Israeli Acute Paralysis Virus (IAPV); Chronic bee paralysis virus (CBPV). The review has been designed to provide researchers in the field with updated information about honeybee viruses and to serve as a starting point for future research. PMID:27800411

  10. Computer Viruses and Safe Educational Practices.

    Science.gov (United States)

    Azarmsa, Reza

    1991-01-01

    This discussion of computer viruses explains how these viruses may be transmitted, describes their effects on data and/or computer application programs, and identifies three groups that propagate them. Ten major viruses are listed and described, and measures to deal with them are discussed. Nineteen antiviral programs are also listed and…

  11. Virus-Like Vesicle-Based Therapeutic Vaccine Vectors for Chronic Hepatitis B Virus Infection

    OpenAIRE

    Tracy D Reynolds; Buonocore, Linda; Rose, Nina F.; Rose, John K.; Robek, Michael D.

    2015-01-01

    More than 500,000 people die each year from the liver diseases that result from chronic hepatitis B virus (HBV) infection. Therapeutic vaccines, which aim to elicit an immune response capable of controlling the virus, offer a potential new treatment strategy for chronic hepatitis B. Recently, an evolved, high-titer vaccine platform consisting of Semliki Forest virus RNA replicons that express the vesicular stomatitis virus glycoprotein (VSV G) has been described. This platform generates virus...

  12. Antigen mimicry involving measles virus hemagglutinin and human respiratory syncytial virus nucleoprotein.

    OpenAIRE

    Norrby, E; Sheshberadaran, H; Rafner, B

    1986-01-01

    Intergenic antigenic relationships between measles virus and respiratory syncytial (RS) virus-specific structural components were studied by using monoclonal antibodies. Of 75 monoclonal antibodies against these components, only one, an anti-measles virus hemagglutinin monoclonal antibody, cross-reacted. Immunofluorescence analysis of measles virus- and RS virus-infected cells with this monoclonal antibody showed qualitatively different staining patterns which indicated that the antigen invol...

  13. Distribution, Statistics, and Resurfacing of Large Impact Basins on Mercury

    Science.gov (United States)

    Fassett, Caleb I.; Head, James W.; Baker, David M. H.; Chapman, Clark R.; Murchie, Scott L.; Neumann, Gregory A.; Oberst, Juergen; Prockter, Louise M.; Smith, David E.; Solomon, Sean C.; Strom, Robert G.; Xiao, Zhiyong; Zuber, Maria T.

    2012-01-01

    The distribution and geological history of large impact basins (diameter D greater than or equal to 300 km) on Mercury is important to understanding the planet's stratigraphy and surface evolution. It is also informative to compare the density of impact basins on Mercury with that of the Moon to understand similarities and differences in their impact crater and basin populations [1, 2]. A variety of impact basins were proposed on the basis of geological mapping with Mariner 10 data [e.g. 3]. This basin population can now be re-assessed and extended to the full planet, using data from the MErcury Surface, Space ENvironment, GEochemistry, and Ranging (MESSENGER) spacecraft. Note that small-to- medium-sized peak-ring basins on Mercury are being examined separately [4, 5]; only the three largest peak-ring basins on Mercury overlap with the size range we consider here. In this study, we (1) re-examine the large basins suggested on the basis of Mariner 10 data, (2) suggest additional basins from MESSENGER's global coverage of Mercury, (3) assess the size-frequency distribution of mercurian basins on the basis of these global observations and compare it to the Moon, and (4) analyze the implications of these observations for the modification history of basins on Mercury.

  14. Evolution of the Rembrandt impact basin on Mercury.

    Science.gov (United States)

    Watters, Thomas R; Head, James W; Solomon, Sean C; Robinson, Mark S; Chapman, Clark R; Denevi, Brett W; Fassett, Caleb I; Murchie, Scott L; Strom, Robert G

    2009-05-01

    MESSENGER's second Mercury flyby revealed a ~715-kilometer-diameter impact basin, the second-largest well-preserved basin-scale impact structure known on the planet. The Rembrandt basin is comparable in age to the Caloris basin, is partially flooded by volcanic plains, and displays a unique wheel-and-spoke-like pattern of basin-radial and basin-concentric wrinkle ridges and graben. Stratigraphic relations indicate a multistaged infilling and deformational history involving successive or overlapping phases of contractional and extensional deformation. The youngest deformation of the basin involved the formation of a approximately 1000-kilometer-long lobate scarp, a product of the global cooling and contraction of Mercury. PMID:19407197

  15. In situ characterization of Hanford K Basins fuel

    International Nuclear Information System (INIS)

    Irradiated N Reactor uranium metal fuel is stored underwater in the Hanford K East and K West Basins. In K East Basin, fuel is stored in open canisters and defected fuel is free to react with the basin water. In K West Basin, the fuel is stored in sealed canisters filled with water containing a corrosion inhibitor (potassium nitrite). To gain a better understanding of the physical condition of the fuel in these basins, visual surveys using high resolution underwater cameras were conducted. The inspections included detailed lift and look examinations of a number of fuel assemblies from selected canisters in each basin. These examinations formed the bases for selecting specific fuel elements for laboratory testing and analyses as prescribed in the characterization plan for Hanford K Basin Spent Nuclear Fuel

  16. Oncogenic viruses and hepatocellular carcinoma.

    Science.gov (United States)

    Ben Ari, Ziv; Weitzman, Ella; Safran, Michal

    2015-05-01

    About 80% of hepatocellular carcinoma (HCC) is caused by hepatitis B virus (HBV) and/or hepatitis C virus (HCV) infections especially in the setting of established cirrhosis or advanced fibrosis, making HCC prevention a major goal of antiviral therapy. HCC tumors are highly complex and heterogeneous resulting from the aberrant function of multiple molecular pathways. The roles of HCV or HBV in promoting HCC development are still either directly or indirectly are still speculative, but the evidence for both effects is compelling. In patients with chronic hepatitis viral infection, cirrhosis is not a prerequisite for tumorigenesis.

  17. Basement faults and volcanic rock distributions in the Ordos Basin

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    Volcanic rocks in the Ordos Basin are of mainly two types: one in the basin and the other along the margin of the basin. Besides those along the margin, the marginal volcanic rocks also include the volcanic rocks in the Yinshanian orogenic belt north of the basin. Based on the latest collection of gravitational and aeromagnetic data, here we interpret basement faults in the Ordos Basin and its peripheral region, compare the faults derived from aeromagnetic data with those from seismic data, and identify the geological ages of the fault development. Two aeromagnetic anomaly zones exist in the NE-trending faults of the southern basin, and they are in the volcanic basement formed in pre-Paleozoic. These NE-trending faults are the channel of volcanic material upwelling in the early age (Archean-Neoproterozoic), where igneous rocks and sedimentary rocks stack successively on both sides of the continental nucleus. In the Cambrian, the basin interior is relatively stable, but in the Late Paleozoic and Mesozoic, the basin margin underwent a number of volcanic activities, accompanied by the formation of nearly north-south and east-west basement faults in the basin periphery and resulting in accumulation of great amount of volcanic materials. Volcanic tuff from the basin periphery is discovered in the central basin and volcanic materials are exposed in the margins of the basin. According to the source-reservoir-cap rock configuration, the basin peripheral igneous traps formed in the Indosinian-Early Yanshanian and Late Hercynian are favorable exploration objectives, and the volcanic rocks in the central basin are the future target of exploration.

  18. Accounting for Basin Effects Will Improve Seismic Risk Assessments

    Science.gov (United States)

    Magistrale, Harold

    2010-05-01

    A fundamental problem in assessing the seismic risk of properties and providing loss prevention solutions is determining the distribution, amplitude, frequency characteristics, and duration of strong ground motion from potential future earthquakes. This task is complicated by the strong effects that three-dimensional (3D) geologic structures, such as sediment-filled basins, have on ground shaking. Numerical studies of seismic wave propagation have shown that ground motions are amplified and have longer durations within basins, and there are observations of basin-edge-generated surface waves and of basin-focusing effects on ground motions. Further, basins typically host deep and/or soft unconsolidated soils that commonly experience enhanced ground motions. Evaluation of the seismic hazards and risks to the basin properties requires 3D numerical ground motion simulations to quantify the contributions of deep basin structure and shallow site conditions to ground motions. These simulations require 3D seismic velocity models. Here, we review these contributions as determined in a mature model of the Los Angeles, California, basin; introduce a new model of the Salt Lake City, Utah, basin; and report on preliminary ground motion simulations in the Salt Lake model. Both models consist of detailed, rule-based representations of the major populated sediment-filled basins, embedded in a 3D crust over a variable depth Moho, over upper mantle velocities. The basins are parameterized as a set of objects and rules implemented in a computer code that generates seismic velocities and density at any desired point. The shallow basin velocities are directly constrained by geotechnical borehole logs and detailed surface site response unit mapping based on surface geology and Vs30 measurements. Based on simulations of a suite of earthquakes in the Los Angeles basin model, Day et al. (2008) model the effect of sedimentary basin depth on long period (2 to 10 s) response spectra. They

  19. Molecular Detection and Characterization of Gastroenteritis Viruses Occurring Naturally in the Stream Waters of Manaus, Central Amazônia, Brazil▿

    OpenAIRE

    Miagostovich, Marize P.; Ferreira, Fabiana F. M.; Guimarães, Flávia R.; Fumian, Túlio M.; Diniz-Mendes, Leonardo; Luz, Sérgio Luiz B.; Luciete A. Silva; Leite, José Paulo G.

    2007-01-01

    To assess the presence of the four main viruses responsible for human acute gastroenteritis in a hydrographic network impacted by a disordered urbanization process, a 1-year study was performed involving water sample collection from streams in the hydrographic basin surrounding the city of Manaus, Amazonas, Brazil. Thirteen surface water sample collection sites, including different areas of human settlement characterized as urban, rural, and primary forest, located in the Tarumã-Açu, São Raim...

  20. Hotspots within the Transboundary Selenga River Basin

    Science.gov (United States)

    Kasimov, Nikolay; Lychagin, Mikhail; Chalov, Sergey

    2013-04-01

    Gathering the efficient information on water pollution of transboundary river systems remains the crucial task in international water management, environmental pollution control and prevention health problems. Countries, located in the low parts of the river basins, depend on the water strategy and water use in the adjacent countries, located upstream. Surface water pollution is considered to be the most serious problem, facing the above-mentioned countries. Large efforts in terms of field measurement campaigns and (numerical) transport modeling are then typically needed for relevant pollution prediction and prevention. Russian rivers take inflow from 8 neighboring countries. Among them there are 2 developing economies - People Republic of China and Mongolia, which are located in water-scarce areas and thus solve their water-related problems through the consumption of international water. Negative change of water runoff and water quality in the foreign part of transboundary river is appeared inside Russian territory with more or less delay. The transboundary river system of Selenga is particularly challenging, being the biggest tributary of Lake Baikal which is the largest freshwater reservoir in the world. Selenga River contributes about 50 % of the total inflow into Baikal. It originates in the mountainous part of Mongolia and then drains into Russia. There are numerous industries and agricultural activities within the Selenga drainage basin that affect the water quality of the river system. Absence of the single monitoring system and predictive tools for pollutants transport in river system requires large efforts in understanding sources of water pollution and implemented data on the relevant numerical systems for the pollution prediction and prevention. Special investigations in the Selenga river basin (Mongolia and Russia) were done to assess hot spots and understand state-of-the art in sediment load, water chemistry and hydrobiology of transboundary systems

  1. Radionuclides in the Great Lakes basin.

    Science.gov (United States)

    Ahier, B A; Tracy, B L

    1995-12-01

    The Great Lakes basin is of radiologic interest due to the large population within its boundaries that may be exposed to various sources of ionizing radiation. Specific radionuclides of interest in the basin arising from natural and artificial sources include 3H, 14C, 90Sr, 129I, 131I, 137Cs, 222Rn, 226Ra, 235U, 238U, 239Pu, and 241Am. The greatest contribution to total radiation exposure is the natural background radiation that provides an average dose of about 2.6 mSv/year to all basin residents. Global fallout from atmospheric nuclear weapons tests conducted before 1963 has resulted in the largest input of anthropogenic radioactivity into the lakes. Of increasing importance is the radionuclide input from the various components of the nuclear fuel cycle. Although the dose from these activities is currently very low, it is expected to increase if there is continued growth of the nuclear industry. In spite of strict regulations on design and operation of nuclear power facilities, the potential exists for a serious accident as a result of the large inventories of radionuclides contained in the reactor cores; however, these risks are several orders of magnitude less than the risks from other natural and man-made hazards. An area of major priority over the next few decades will be the management of the substantial amounts of radioactive waste generated by nuclear fuel cycle activities. Based on derived risk coefficients, the theoretical incidence of fatal and weighted nonfatal cancers and hereditary defects in the basin's population, attributable to 50 years of exposure to natural background radiation, is conservatively estimated to be of the order of 3.4 x 10(5) cases. The total number of attributable health effects to the year 2050 from fallout radionuclides in the Great Lakes basin is of the order of 5.0 x 10(3). In contrast, estimates of attributable health effects from 50 years of exposure to current nuclear fuel cycle effluent in the basin are of the order of 2

  2. Hyperthermophilic Archaeal Viruses as Novel Nanoplatforms

    DEFF Research Database (Denmark)

    Uldahl, Kristine Buch

    ; attachment, alignment, and fusion. Upon infection, the intracellular replication cycle lasts 8 h at which point the virus particles are released as spindle-shaped tailless particles. Chapter II builds on the replication and purification methods in Chapter I to study the interaction between the two...... nanoplatforms than mammalian viruses because they cannot proliferate in humans and hence are less likely to trigger adverse effects. Another group of viruses that fits this criterion is archaeal viruses yet their potential remains untapped. As a group, archaeal viruses offer distinct advantages such as unique...

  3. A new looming of Zika virus

    Directory of Open Access Journals (Sweden)

    Nirav R. Soni

    2016-05-01

    Full Text Available Zika virus (ZIKV is a member of the virus family Flaviviridae and the genus Flavivirus, transmitted by daytime-active Aedes mosquitoes, such as A. aegypti. ZIKV will continue to spread and it will be difficult to determine how the virus will spread over time. Sign and symptoms of ZIKAVD (Zika virus disease were conjunctivitis (red eyes, back pain, birth defect-abnormal brain development known as microcephaly and it is diagnosed through PCR (polymerase chain reaction and virus isolation from blood samples.

  4. Radiation Inactivation of Viruses in Infected Products

    International Nuclear Information System (INIS)

    Full text: The effects of gamma radiation on foot-and-mouth disease virus in vitro and in situ have been studied. The data so far obtained show that a dose of 2 Mrad is required to inactivate virus in infected animal carcasses. But the dose may adversely affect the organoleptic quality of the meat. Experiments in vitro and in situ are necessary to study the effects of ionizing radiation on other viruses, such as rinderpest, swine fever and African swine fever-viruses, associated with animal products. Radiation may offer a possible means of eliminating the virus titre in many animal products and solve consequent quarantine problems. (author)

  5. Identification of Potato Virus Y Strains in Tobacco Crops

    OpenAIRE

    Jelena Zindović; Janoš Berenji; Milena Pauković; Ivana Đekić; Aleksandra Bulajić; Branka Krstić

    2007-01-01

    Five viruses: Potato Virus Y (PVY), Tomato Spotted Wilt Virus, Cucumber Mosaic Virus, Tobacco Mosaic Virus and Alfalfa Mosaic Virus, of which PVY was predominant, were detected by serological testing of tobacco samples collected from many localities in Vojvodina in 2006. Viruses are the most important pathogens in tobacco and PVY causes considerable economic damages all over the world. A PVY population comprises several different strain groups, strain subgroups and recombinant strains. Among ...

  6. Presence and distribution of economically important potato viruses in Montenegro

    OpenAIRE

    Jelena Zindović

    2011-01-01

    The research was carried out, in the period 2002-2004 in order to determine the presence and distribution of potato viruses at 12 different locations and on 9 different potato varieties grown in Montenegro. The research included collecting of samples in seed potato crops and testing of six economically important potato viruses: Potato leaf roll virus (PLRV), Potato virus Y (PVY), Potato virus X (PVX), Potato virus S (PVS), Potato virus A (PVA) i Potato viru...

  7. Method for detecting viruses in aerosols.

    Science.gov (United States)

    Wallis, C; Melnick, J L; Rao, V C; Sox, T E

    1985-11-01

    A simple method with poliovirus as the model was developed for recovering human enteric viruses from aerosols. Filterite filters (pore size, 0.45 micron; Filterite Corp., Timonium, Md.) moistened with glycine buffer (pH 3.5) were used for adsorbing the aerosolized virus. No virus passed the filter, even with air flow rates of 100 liters/min. Virus recovery from the filter was achieved by rapid elution with 800 ml of glycine buffer, pH 10. The virus in the primary eluate was reconcentrated by adjusting the pH to 3.5, adding AlCl3 to 0.0005 M, collecting the virus on a 0.25-micron-pore Filerite disk (diameter, 25 mm) and and eluting with 6 ml of buffer, pH 10. With this method, virus could be detected regularly in aerosols produced by flushing when 3 X 10(8) PFU of poliovirus were present in the toilet bowl. Poliovirus-containing fecal material from two of four infants who had recently received oral polio vaccine also yielded virus in the aerosols when feces containing 2.4 X 10(7) to 4.5 X 10(7) PFU of virus had been added to the toilet bowl. Persons infected with a variety of natural enteric viruses are known to excrete this amount of virus in their daily stools. PMID:3004329

  8. Sensitiveness of viruses to gamma radiation

    International Nuclear Information System (INIS)

    The sensitiveness of viruses to gamma rays was compared using eight viruses suspended with low concentration in drinking water, and four viruses present in high concentrations in tissue culture medium. The results show that the following factors are responsible for the resistance of viruses to gamma rays: 1. type of virus: the specific radiation resistance varied considerably; in general, there was a closer correlation with the general resistance of the virus to chemico-physical influences than with the type of nucleic acid of the virus examined; 2. medium of suspension and state of aggregation: high protein content and lyophilisation increased the resistance to gamma rays widely; 3. virus concentration: the virus reduction by a factor of 10 in suspensions with high virus concentration needed a higher radiation dose compared with suspensions of low virus content. All the results demonstrate the kinetics of inactivation to be a 1st order reaction. The increase of temperature to 410C did not show any significant influence. (orig.)

  9. Comparative analysis of chrysanthemum transcriptome in response to three RNA viruses: Cucumber mosaic virus, Tomato spotted wilt virus and Potato virus X.

    Science.gov (United States)

    Choi, Hoseong; Jo, Yeonhwa; Lian, Sen; Jo, Kyoung-Min; Chu, Hyosub; Yoon, Ju-Yeon; Choi, Seung-Kook; Kim, Kook-Hyung; Cho, Won Kyong

    2015-06-01

    The chrysanthemum is one of popular flowers in the world and a host for several viruses. So far, molecular interaction studies between the chrysanthemum and viruses are limited. In this study, we carried out a transcriptome analysis of chrysanthemum in response to three different viruses including Cucumber mosaic virus (CMV), Tomato spotted wilt virus (TSWV) and Potato virus X (PVX). A chrysanthemum 135K microarray derived from expressed sequence tags was successfully applied for the expression profiles of the chrysanthemum at early stage of virus infection. Finally, we identified a total of 125, 70 and 124 differentially expressed genes (DEGs) for CMV, TSWV and PVX, respectively. Many DEGs were virus specific; however, 33 DEGs were commonly regulated by three viruses. Gene ontology (GO) enrichment analysis identified a total of 132 GO terms, and of them, six GO terms related stress response and MCM complex were commonly identified for three viruses. Several genes functioning in stress response such as chitin response and ethylene mediated signaling pathway were up-regulated indicating their involvement in establishment of host immune system. In particular, TSWV infection significantly down-regulated genes related to DNA metabolic process including DNA replication, chromatin organization, histone modification and cytokinesis, and they are mostly targeted to nucleosome and MCM complex. Taken together, our comparative transcriptome analysis revealed several genes related to hormone mediated viral stress response and DNA modification. The identified chrysanthemums genes could be good candidates for further functional study associated with resistant to various plant viruses. PMID:25904110

  10. Mayaro Fever Virus, Brazilian Amazon

    OpenAIRE

    Azevedo, Raimunda S. S.; Silva, Eliana V. P.; Carvalho, Valéria L.; Rodrigues, Sueli G.; Neto, Joaquim P. Nunes; Monteiro, Hamilton A. de O.; Peixoto, Victor S.; Chiang, Jannifer O.; Nunes, Márcio R. T.; Vasconcelos, Pedro F.C.

    2009-01-01

    In February 2008, a Mayaro fever virus (MAYV) outbreak occurred in a settlement in Santa Barbara municipality, northern Brazil. Patients had rash, fever, and severe arthralgia lasting up to 7 days. Immunoglobulin M against MAYV was detected by ELISA in 36 persons; 3 MAYV isolates sequenced were characterized as genotype D.

  11. Oncolytic viruses as anticancer vaccines

    Directory of Open Access Journals (Sweden)

    Norman eWoller

    2014-07-01

    Full Text Available Oncolytic virotherapy has shown impressive results in preclinical studies and first promising therapeutic outcomes in clinical trials as well. Since viruses are known for a long time as excellent vaccination agents, oncolytic viruses are now designed as novel anticancer agents combining the aspect of lysis-dependent cytoreductive activity with concomitant induction of antitumoral immune responses. Antitumoral immune activation by oncolytic virus infection of tumor tissue comprises both, immediate effects of innate immunity and also adaptive responses for long lasting antitumoral activity which is regarded as the most prominent challenge in clinical oncology. To date, the complex effects of a viral tumor infection on the tumor microenvironment and the consequences for the tumor-infiltrating immune cell compartment are poorly understood. However, there is more and more evidence that a tumor infection by an oncolytic virus opens up a number of options for further immunomodulating interventions such as systemic chemotherapy, generic immunostimulating strategies, dendritic cell-based vaccines, and antigenic libraries to further support clinical efficacy of oncolytic virotherapy.

  12. Control of feline leukaemia virus.

    NARCIS (Netherlands)

    K. Weijer (Kees); F.G.C.M. Uytdehaag (Fons); A.D.M.E. Osterhaus (Ab)

    1989-01-01

    textabstractFeline leukaemia virus (FeLV) usually occurs in its natural species, the domestic cat. FeLV is also important to human individuals as a comparative model, as it may cause a variety of diseases, some malignant and some benign, such as immunosuppression, which bears a resemblance to AIDS (

  13. Bat Flight and Zoonotic Viruses

    Centers for Disease Control (CDC) Podcasts

    2014-05-30

    Reginald Tucker reads an abridged version of the EID perspective Bat Flight and Zoonotic Viruses.  Created: 5/30/2014 by National Center for Emerging and Zoonotic Infectious Diseases (NCEZID).   Date Released: 6/2/2014.

  14. Threat from Emerging Vectorborne Viruses

    Centers for Disease Control (CDC) Podcasts

    2016-06-09

    Reginald Tucker reads an abridged version of the commentary by CDC author Ronald Rosenberg, Threat from Emerging Vectorborne Viruses.  Created: 6/9/2016 by National Center for Emerging and Zoonotic Infectious Diseases (NCEZID).   Date Released: 6/9/2016.

  15. A Case of Ebola Virus

    Centers for Disease Control (CDC) Podcasts

    2012-10-01

    Dr. Adam MacNeil, an epidemiologist at CDC, discusses Ebola virus.  Created: 10/1/2012 by National Center for Emerging and Zoonotic Infectious Diseases (NCEZID); National Center for Immunization and Respiratory Diseases (NCIRD).   Date Released: 10/1/2012.

  16. Transplacental transmission of bluetongue virus

    NARCIS (Netherlands)

    van der Sluijs, M.T.W

    2014-01-01

    Bluetongue is an economically important disease of ruminants. The causative agent, Bluetongue virus (BTV), is mainly transmitted by insect vectors. The research described in this thesis focuses on vector-independent BTV transmission, and its epizootic and economic consequences. Vector-independent tr

  17. Enumerating viruses in coral mucus

    OpenAIRE

    Leruste, Amandine; Bouvier, Thierry; Bettarel, Yvan

    2012-01-01

    The distribution of viruses inhabiting the coral mucus remains undetermined, as there is no suitable standardized procedure for their separation from this organic matrix, principally owing to its viscosity and autofluorescence. Seven protocols were tested, and the most efficient separations were obtained from a chemical treatment requiring potassium citrate.

  18. Enumerating viruses in coral mucus.

    Science.gov (United States)

    Leruste, Amandine; Bouvier, Thierry; Bettarel, Yvan

    2012-09-01

    The distribution of viruses inhabiting the coral mucus remains undetermined, as there is no suitable standardized procedure for their separation from this organic matrix, principally owing to its viscosity and autofluorescence. Seven protocols were tested, and the most efficient separations were obtained from a chemical treatment requiring potassium citrate. PMID:22729548

  19. Molecular phylogeny of Duvenhage virus

    Directory of Open Access Journals (Sweden)

    Louis H. Nel

    2011-11-01

    Full Text Available The Duvenhage virus (DUVV constitutes one of the 11 species in the Lyssavirus genus and causes fatal rabies encephalitis. The virus is associated with insectivorous bat species and three human cases have been reported, all of which were linked to contact with bats. Few of these isolates have been studied and thus little is known about the phylogeny and epidemiology of this lyssavirus. Until 2007, when an isolate was made from the East African country of Kenya, all isolations of this virus had been from southern Africa. This discovery led to many questions regarding the spread and diversity of this lyssavirus. Phylogenetic analysis indicated that the DUVV isolates constitute two different lineages, in which the southern African isolates group together to form one lineage and the more recent isolate from Kenya constitutes a new, second lineage. We found that the new isolate has a genetic variation that has not yet been seen for DUVV. Not only is our lack of knowledge regarding the geographical distribution of this uniquely African virus emphasised, but we have also demonstrated the potential diversity within this genotype.

  20. Mutants of alfalfa mosaic virus

    International Nuclear Information System (INIS)

    In this thesis the isolation and characterization of a number of mutants of alfalfa mosaic virus, a plant virus with a coat protein dependent genome, is described. Thermo-sensitive (ts) mutants were selected since, at least theoretically, ts mutations can be present in all virus coded functions. It was found that a high percentage of spontaneous mutants, isolated because of their aberrant symptoms, were ts. The majority of these isolates could grow at the non-permissive temperature in the presence of a single wild type (wt) component. To increase the mutation rate virus preparations were treated with several mutagens. After nitrous acid treatment or irradiation with ultraviolet light, an increase in the level of mutations was observed. UV irradiation was preferred since it did not require large amounts of purified viral components. During the preliminary characterization of potential ts mutants the author also obtained one structural and several symptom mutants which were analysed further (chapter 7, 8 and 9). The properties of the ts mutants are described in chapter 3-7. (Auth.)