WorldWideScience

Sample records for basic preanalytical principles

  1. Translating molecular medicine into clinical tools: doomed to fail by neglecting basic preanalytical principles

    Directory of Open Access Journals (Sweden)

    Mannello Ferdinando

    2009-10-01

    Full Text Available Abstract This commentary discusses a study on measurements of matrix metalloproteinase 9 (MMP-9 in serum of pseudoxanthoma elasticum patients recently published in Journal of Molecular Medicine. This study can be considered the typical "obstacle" to effective translational medicine as previously documented in JTM journal. Although serum has been frequently proven as inappropriate sample for determining numerous circulating MMPs, among them MMP-9, there are over and over again studies, as in this case, that measure MMP-9 in serum. Comparative measurements in serum and plasma samples demonstrated higher concentrations for MMP-9 in serum due to the additional release from leukocytes and platelets following the coagulation/fibrinolysis process. From this example it can be concluded that translating basic research discoveries into clinical tools needs a more intensive exchange between basic biomedical research and clinical scientists already in an early stage. Otherwise a lost of translation, as discussed in JTM journal, seems to be inevitable.

  2. Improving preanalytic processes using the principles of lean production (Toyota Production System).

    Science.gov (United States)

    Persoon, Thomas J; Zaleski, Sue; Frerichs, Janice

    2006-01-01

    The basic technologies used in preanalytic processes for chemistry tests have been mature for a long time, and improvements in preanalytic processes have lagged behind improvements in analytic and postanalytic processes. We describe our successful efforts to improve chemistry test turnaround time from a central laboratory by improving preanalytic processes, using existing resources and the principles of lean production. Our goal is to report 80% of chemistry tests in less than 1 hour and to no longer recognize a distinction between expedited and routine testing. We used principles of lean production (the Toyota Production System) to redesign preanalytic processes. The redesigned preanalytic process has fewer steps and uses 1-piece flow to move blood samples through the accessioning, centrifugation, and aliquoting processes. Median preanalytic processing time was reduced from 29 to 19 minutes, and the laboratory met the goal of reporting 80% of chemistry results in less than 1 hour for 11 consecutive months.

  3. Basic principles

    International Nuclear Information System (INIS)

    Wilson, P.D.

    1996-01-01

    Some basic explanations are given of the principles underlying the nuclear fuel cycle, starting with the physics of atomic and nuclear structure and continuing with nuclear energy and reactors, fuel and waste management and finally a discussion of economics and the future. An important aspect of the fuel cycle concerns the possibility of ''closing the back end'' i.e. reprocessing the waste or unused fuel in order to re-use it in reactors of various kinds. The alternative, the ''oncethrough'' cycle, discards the discharged fuel completely. An interim measure involves the prolonged storage of highly radioactive waste fuel. (UK)

  4. Evaluation of Preanalytical Quality Indicators by Six Sigma and Pareto`s Principle.

    Science.gov (United States)

    Kulkarni, Sweta; Ramesh, R; Srinivasan, A R; Silvia, C R Wilma Delphine

    2018-01-01

    Preanalytical steps are the major sources of error in clinical laboratory. The analytical errors can be corrected by quality control procedures but there is a need for stringent quality checks in preanalytical area as these processes are done outside the laboratory. Sigma value depicts the performance of laboratory and its quality measures. Hence in the present study six sigma and Pareto principle was applied to preanalytical quality indicators to evaluate the clinical biochemistry laboratory performance. This observational study was carried out for a period of 1 year from November 2015-2016. A total of 1,44,208 samples and 54,265 test requisition forms were screened for preanalytical errors like missing patient information, sample collection details in forms and hemolysed, lipemic, inappropriate, insufficient samples and total number of errors were calculated and converted into defects per million and sigma scale. Pareto`s chart was drawn using total number of errors and cumulative percentage. In 75% test requisition forms diagnosis was not mentioned and sigma value of 0.9 was obtained and for other errors like sample receiving time, stat and type of sample sigma values were 2.9, 2.6, and 2.8 respectively. For insufficient sample and improper ratio of blood to anticoagulant sigma value was 4.3. Pareto`s chart depicts out of 80% of errors in requisition forms, 20% is contributed by missing information like diagnosis. The development of quality indicators, application of six sigma and Pareto`s principle are quality measures by which not only preanalytical, the total testing process can be improved.

  5. Emulsion Science Basic Principles

    CERN Document Server

    Leal-Calderon, Fernando; Schmitt, Véronique

    2007-01-01

    Emulsions are generally made out of two immiscible fluids like oil and water, one being dispersed in the second in the presence of surface-active compounds.They are used as intermediate or end products in a huge range of areas including the food, chemical, cosmetic, pharmaceutical, paint, and coating industries. Besides the broad domain of technological interest, emulsions are raising a variety of fundamental questions at the frontier between physics and chemistry. This book aims to give an overview of the most recent advances in emulsion science. The basic principles, covering aspects of emulsions from their preparation to their destruction, are presented in close relation to both the fundamental physics and the applications of these materials. The book is intended to help scientists and engineers in formulating new materials by giving them the basics of emulsion science.

  6. Thermionics basic principles of electronics

    CERN Document Server

    Jenkins, J; Ashhurst, W

    2013-01-01

    Basic Principles of Electronics, Volume I : Thermionics serves as a textbook for students in physics. It focuses on thermionic devices. The book covers topics on electron dynamics, electron emission, and the themionic vacuum diode and triode. Power amplifiers, oscillators, and electronic measuring equipment are studied as well. The text will be of great use to physics and electronics students, and inventors.

  7. Basic safety principles: Lessons learned

    Energy Technology Data Exchange (ETDEWEB)

    Erp, J.B. van [Argonne National Lab., IL (United States)

    1997-09-01

    The presentation reviews the following issues: basic safety principles and lessons learned; some conclusions from the Kemeny report on the accident at TMI; some recommendations from the Kemeny report on the accident at TMI; conclusions and recommendations from the Rogovin report on the accident on TMI; instrumentation deficiencies (from Rogovin report).

  8. Photodynamic Therapy (PDT) - Basic Principles

    Indian Academy of Sciences (India)

    Home; Journals; Resonance – Journal of Science Education; Volume 5; Issue 4. Photodynamic Therapy (PDT) - Basic Principles. Bhaskar G Maiya. Series Article Volume 5 Issue 4 April 2000 pp 6-18. Fulltext. Click here to view fulltext PDF. Permanent link: https://www.ias.ac.in/article/fulltext/reso/005/04/0006-0018 ...

  9. Basic safety principles: Lessons learned

    International Nuclear Information System (INIS)

    Erp, J.B. van

    1997-01-01

    The presentation reviews the following issues: basic safety principles and lessons learned; some conclusions from the Kemeny report on the accident at TMI; some recommendations from the Kemeny report on the accident at TMI; conclusions and recommendations from the Rogovin report on the accident on TMI; instrumentation deficiencies (from Rogovin report)

  10. Basic Principles of Wastewater Treatment

    OpenAIRE

    Von Sperling, Marcos

    2007-01-01

    "Basic Principles of Wastewater Treatment is the second volume in the series Biological Wastewater Treatment, and focusses on the unit operations and processes associated with biological wastewater treatment. The major topics covered are: microbiology and ecology of wastewater treatment reaction kinetics and reactor hydraulics conversion of organic and inorganic matter sedimentation aeration The theory presented in this volume forms the basis upon which the other books...

  11. Positron emission tomography. Basic principles

    International Nuclear Information System (INIS)

    Rodriguez, Jose Luis; Massardo, Teresa; Gonzalez, Patricio

    2001-01-01

    The basic principles of positron emission tomography (PET) technique are reviewed. lt allows to obtain functional images from gamma rays produced by annihilation of a positron, a positive beta particle. This paper analyzes positron emitters production in a cyclotron, its general mechanisms, and the various detection systems. The most important clinical applications are also mentioned, related to oncological uses of fluor-l8-deoxyglucose

  12. Experimental toxicology: the basic principles

    National Research Council Canada - National Science Library

    Anderson, Diana; Conning, D. M

    1988-01-01

    Principles and methods are discussed in detail, covering experimental design, biochemical issues, animal husbandry, species differences, immunological issues, carcinogenesis, reproductive approaches...

  13. 5 CFR 551.401 - Basic principles.

    Science.gov (United States)

    2010-01-01

    ... 5 Administrative Personnel 1 2010-01-01 2010-01-01 false Basic principles. 551.401 Section 551.401 Administrative Personnel OFFICE OF PERSONNEL MANAGEMENT CIVIL SERVICE REGULATIONS PAY ADMINISTRATION UNDER THE FAIR LABOR STANDARDS ACT Hours of Work General Provisions § 551.401 Basic principles. (a) All time...

  14. Basic principles of concrete structures

    CERN Document Server

    Gu, Xianglin; Zhou, Yong

    2016-01-01

    Based on the latest version of designing codes both for buildings and bridges (GB50010-2010 and JTG D62-2004), this book starts from steel and concrete materials, whose properties are very important to the mechanical behavior of concrete structural members. Step by step, analysis of reinforced and prestressed concrete members under basic loading types (tension, compression, flexure, shearing and torsion) and environmental actions are introduced. The characteristic of the book that distinguishes it from other textbooks on concrete structures is that more emphasis has been laid on the basic theories of reinforced concrete and the application of the basic theories in design of new structures and analysis of existing structures. Examples and problems in each chapter are carefully designed to cover every important knowledge point. As a basic course for undergraduates majoring in civil engineering, this course is different from either the previously learnt mechanics courses or the design courses to be learnt. Compa...

  15. Basic photovoltaic principles and methods

    Energy Technology Data Exchange (ETDEWEB)

    Hersch, P.; Zweibel, K.

    1982-02-01

    This book presents a nonmathematical explanation of the theory and design of photovoltaic (PV) solar cells and systems. The basic elements of PV are introduced: the photovoltaic effect, physical aspects of solar cell efficiency, the typical single-crystal silicon solar cell, advances in single-crystal silicon solar cells. This is followed by the designs of systems constructed from individual cells, including possible constructions for putting cells together and the equipment needed for a practical producer of electrical energy. The future of PV is then discussed. (LEW)

  16. Basic safety principles for nuclear power plant

    International Nuclear Information System (INIS)

    Zhang Shiguan

    1989-01-01

    To ensure the safety operation of nuclear power plant, one should strictly adhere to the implelmentation of safety codes and the establishment of nuclear safety code system, as well as the applicable basic safety principles of nuclear power plants. This article briefly introduce the importance of nuclear codes and its economic benefits and the implementation of basic safety principles to be accumulated in practice for many years by various countries

  17. Basic principles of quality assurance

    International Nuclear Information System (INIS)

    Stauffer, M.

    1977-01-01

    After a brief review of the origin of the 'quality concept' and the historical development of quality assurance, questions such as 'what is QA' and 'why is QA so important in nuclear technology' as well as definitions and main requirements of relevant QA codes and standards are presented and discussed. By means of a project realization schematic, tasks, duties, responsibilities, and possible QA organigrammes as well as QA programme and manual requirements are explained and compared. From a QA point of view, it is shown that no basic difference exists between design and production or construction control activities. Special emphasis is layed upon active owner's participation in the implementation of QA programmes for NPP and the advantages offered are described and illustrated by typical examples. (RW) [de

  18. Basic Principles of Animal Science. Reprinted.

    Science.gov (United States)

    Florida State Dept. of Education, Tallahassee.

    The reference book is designed to fulfill the need for organized subject matter dealing with basic principles of animal science to be incorporated into the high school agriculture curriculum. The material presented is scientific knowledge basic to livestock production. Five units contain specific information on the following topics: anatomy and…

  19. Phase equilibria basic principles, applications, experimental techniques

    CERN Document Server

    Reisman, Arnold

    2013-01-01

    Phase Equilibria: Basic Principles, Applications, Experimental Techniques presents an analytical treatment in the study of the theories and principles of phase equilibria. The book is organized to afford a deep and thorough understanding of such subjects as the method of species model systems; condensed phase-vapor phase equilibria and vapor transport reactions; zone refining techniques; and nonstoichiometry. Physicists, physical chemists, engineers, and materials scientists will find the book a good reference material.

  20. Behavior Modification: Basic Principles. Third Edition

    Science.gov (United States)

    Lee, David L.; Axelrod, Saul

    2005-01-01

    This classic book presents the basic principles of behavior emphasizing the use of preventive techniques as well as consequences naturally available in the home, business, or school environment to change important behaviors. This book, and its companion piece, "Measurement of Behavior," represents more than 30 years of research and strategies in…

  1. Basic principles simulators - concept training simulators

    International Nuclear Information System (INIS)

    Benkert, J.

    1986-01-01

    Basic Principles Simulators have the purpose of teaching general concepts, demonstrating and displaying the fundamental physical processes of a plant. They are used to illustrate theory to students and also to provide a preliminary training to the operators, to aquaint them with the basic dynamic interactions of the various systems during the normal operation of a plant, and to show the consequences of the most important and common transients and malfunctions. Basic principles simulators may vary in size from small desk cabinets to large panels. They represent with a certain detail the nuclear and thermohydraulic part of the plant. The availability of video displays allows to present detailed information about process parameters which are not shown on the control panels. In general the overall plant behaviour is represented well. Limitations are mostly found in the areas of logic and control. (orig./HP)

  2. Basic safety principles for nuclear power plants

    International Nuclear Information System (INIS)

    1988-01-01

    Nuclear power plant safety requires a continuing quest for excellence. All individuals concerned should constantly be alert to opportunities to reduce risks to the lowest practicable level. The quest, however, is most likely to be fruitful if it is based on an understanding of the underlying objectives and principles of nuclear safety, and the way in which its aspects are interrelated. This report is an attempt to provide a logical framework for such an understanding. The proposed objectives and principles of nuclear safety are interconnected and must be taken as a whole; they do not constitute a menu from which selection can be made. The report takes account of current issues and developments. It includes the concept of safety objectives and the use of probabilistic safety assessment. Reliability targets for safety systems are discussed. The concept of a 'safety culture' is crucial. Attention has been paid to the need for planning for accident management. The report contains objectives and principles. The objectives state what is to be achieved; the principles state how to achieve it. In each case, the basic principle is stated as briefly as possible. The accompanying discussion comments on the reasons for the principle and its importance, as well as exceptions, the extent of coverage and any necessary clarification. The discussion is as important as the principle it augments. 4 figs

  3. Basic principles of nuclear magnetic resonance imaging

    International Nuclear Information System (INIS)

    Valk, J.; MacLean, C.; Algra, P.R.

    1985-01-01

    The intent of this book is to help clinicians understand the basic physical principles of magnetic resonance (MR) imaging. The book consists of the following: a discussion of elementary considerations; pulse sequencing; localization of MR signals in space; MR equipment; MR contrast agents; clinical applications; MR spectroscopy; and biological effects of MR imaging; a set of appendixes; and a bibliography. Illustrations and images are included

  4. Basic principles of radiation protection in Canada

    International Nuclear Information System (INIS)

    1990-03-01

    The major goal of radiation protection in Canada is to ensure that individuals are adequately protected against the harm that might arise from unwarranted exposure to ionizing radiation. This report deals with the basic principles and organizations involved in protection against ionizing radiation. Three basic principles of radiation protection are: 1) that no practice shall be adopted unless its introduction produces a positive net benefit for society, 2) that all exposures shall be kept as low as reasonably achievable, relevant economic and social factors being taken into account, and 3) that doses to individuals should not exceed specified annual limits. The limit for radiation workers is currently 50 mSv per year, and exposures of the general public should not exceed a small fraction of that of radiation workers. Other specific areas in radiation protection which have received considerable attention in Canada include limitations on collective dose (the sum of the individual doses for all exposed individuals), exemption rules for extremely small radiation doses or amounts of radioactive materials, occupational hazards in uranium mining, and special rules for protection of the foetus in pregnant female radiation workers. Implementation of radiation protection principles in Canada devolves upon the Atomic Energy Control Board, the Department of National Health and Welfare, provincial authorities, licensees and radiation workers. A brief description is given of the roles of each of these groups

  5. Implementation of a basic principle simulator

    International Nuclear Information System (INIS)

    De Greef, J.F.

    1982-01-01

    During the last twenty years, the Belgian Nuclear Energy Centre (SCK-CEN) gained a lot of experience in mathematical modelling and simulation of nuclear reactor power plant operation. The framework, in which these activities took place, was mainly concerned with security analysis of these devices and with training operation personnel of these plants. In the last years a fourth generation hybrid computer including an AD/FIVE analog computer of Applied Dynamics Inc. (ADI) and a PDP-11/34 digital computer of Digital Equipment Corporation (DEC) were installed. Furthermore, a home made operator's desk simulating the elements of the control room of a nuclear power plant completes the computer system for real-time training purposes. The described equipment is typically that of a Basic Principle Simulator, a device that quickly and effectively imparts the basic concepts of a nuclear power plant operation

  6. Basic principle of cone beam computed tomography

    International Nuclear Information System (INIS)

    Choi, Yong Suk; Kim, Gyu Tae; Hwang, Eui Hwan

    2006-01-01

    The use of computed tomography for dental procedures has increased recently. Cone beam computed tomography(CBCT) systems have been designed for imaging hard tissues of the dentomaxillofacial region. CBCT is capable of providing high resolution in images of high diagnostic quality. This technology allows for 3-dimensional representation of the dentomaxillofacial skeleton with minimal distortion, but at lower equipment cost, simpler image acquisition and lower patient dose. Because this technology produces images with isotropic sub-millimeter spatial resolution, it is ideally suited for dedicated dentomaxillofacial imaging. In this paper, we provide a brief overview of cone beam scanning technology and compare it with the fan beam scanning used in conventional CT and the basic principles of currently available CBCT systems

  7. Basic design principles of colorimetric vision systems

    Science.gov (United States)

    Mumzhiu, Alex M.

    1998-10-01

    Color measurement is an important part of overall production quality control in textile, coating, plastics, food, paper and other industries. The color measurement instruments such as colorimeters and spectrophotometers, used for production quality control have many limitations. In many applications they cannot be used for a variety of reasons and have to be replaced with human operators. Machine vision has great potential for color measurement. The components for color machine vision systems, such as broadcast quality 3-CCD cameras, fast and inexpensive PCI frame grabbers, and sophisticated image processing software packages are available. However the machine vision industry has only started to approach the color domain. The few color machine vision systems on the market, produced by the largest machine vision manufacturers have very limited capabilities. A lack of understanding that a vision based color measurement system could fail if it ignores the basic principles of colorimetry is the main reason for the slow progress of color vision systems. the purpose of this paper is to clarify how color measurement principles have to be applied to vision systems and how the electro-optical design features of colorimeters have to be modified in order to implement them for vision systems. The subject of this presentation far exceeds the limitations of a journal paper so only the most important aspects will be discussed. An overview of the major areas of applications for colorimetric vision system will be discussed. Finally, the reasons why some customers are happy with their vision systems and some are not will be analyzed.

  8. Basic principles of fracture treatment in children.

    Science.gov (United States)

    Ömeroğlu, Hakan

    2018-04-01

    This review aims to summarize the basic treatment principles of fractures according to their types and general management principles of special conditions including physeal fractures, multiple fractures, open fractures, and pathologic fractures in children. Definition of the fracture is needed for better understanding the injury mechanism, planning a proper treatment strategy, and estimating the prognosis. As the healing process is less complicated, remodeling capacity is higher and non-union is rare, the fractures in children are commonly treated by non-surgical methods. Surgical treatment is preferred in children with multiple injuries, in open fractures, in some pathologic fractures, in fractures with coexisting vascular injuries, in fractures which have a history of failed initial conservative treatment and in fractures in which the conservative treatment has no/little value such as femur neck fractures, some physeal fractures, displaced extension and flexion type humerus supracondylar fractures, displaced humerus lateral condyle fractures, femur, tibia and forearm shaft fractures in older children and adolescents and unstable pelvis and acetabulum fractures. Most of the fractures in children can successfully be treated by non-surgical methods.

  9. Basic principles of molecular effects of irradiation.

    Science.gov (United States)

    Selzer, Edgar; Hebar, Alexandra

    2012-02-01

    In order to understand the consequences of radiation a thorough understanding of the radiobiological mechanisms of the molecular up to the clinical level is of importance. Radiobiology therefore combines the basic principles of physics as well as biology and medicine and is concerned with the action of radiation from the subcellular level up to the living organism. Topics of interest and relevance are covered in much more broadness as is possible in the short following article in the literature to which the interested reader is referred to. Classical books in this field were written by Steel et al. (1989) as well as by Hall (1994). Topics usually covered by radiobiological reviews are the classification of different types of radiation, cell cycle dependency of radiation effects, types of radiation damage and cell death, dose response curves, measurement of radiation damage, the oxygen effect, relative biological effectiveness, the influence of dose rate, and several other important research areas. This short overview will concentrate on a subset of radiobiological topics of high importance and relative novelty.

  10. Preanalytics in lung cancer.

    Science.gov (United States)

    Warth, Arne; Muley, Thomas; Meister, Michael; Weichert, Wilko

    2015-01-01

    Preanalytic sampling techniques and preparation of tissue specimens strongly influence analytical results in lung tissue diagnostics both on the morphological but also on the molecular level. However, in contrast to analytics where tremendous achievements in the last decade have led to a whole new portfolio of test methods, developments in preanalytics have been minimal. This is specifically unfortunate in lung cancer, where usually only small amounts of tissue are at hand and optimization in all processing steps is mandatory in order to increase the diagnostic yield. In the following, we provide a comprehensive overview on some aspects of preanalytics in lung cancer from the method of sampling over tissue processing to its impact on analytical test results. We specifically discuss the role of preanalytics in novel technologies like next-generation sequencing and in the state-of the-art cytology preparations. In addition, we point out specific problems in preanalytics which hamper further developments in the field of lung tissue diagnostics.

  11. Preanalytical requirements of urinalysis

    Science.gov (United States)

    Delanghe, Joris; Speeckaert, Marijn

    2014-01-01

    Urine may be a waste product, but it contains an enormous amount of information. Well-standardized procedures for collection, transport, sample preparation and analysis should become the basis of an effective diagnostic strategy for urinalysis. As reproducibility of urinalysis has been greatly improved due to recent technological progress, preanalytical requirements of urinalysis have gained importance and have become stricter. Since the patients themselves often sample urine specimens, urinalysis is very susceptible to preanalytical issues. Various sampling methods and inappropriate specimen transport can cause important preanalytical errors. The use of preservatives may be helpful for particular analytes. Unfortunately, a universal preservative that allows a complete urinalysis does not (yet) exist. The preanalytical aspects are also of major importance for newer applications (e.g. metabolomics). The present review deals with the current preanalytical problems and requirements for the most common urinary analytes. PMID:24627718

  12. Basic principles of solar water heating

    CSIR Research Space (South Africa)

    Page-Shipp, RJ

    1980-09-10

    Full Text Available This article correctly reflects the principles of Solar Water Heating as they pertain to South African conditions. However, it was written in 1980 and the global energy situation has changed considerably. Furthermore, modern commercial units...

  13. Basic Concepts and Principles of Marketing.

    Science.gov (United States)

    Beder, Hal

    1986-01-01

    Presents an overview of marketing concepts and principles. These include (1) organizational objectives, (2) exchange, (3) value, (4) market segmentation, (5) market position, (6) consumer analysis, (7) product, (8) promotion, (9) place, and (10) price. (CH)

  14. Irrigation water management: Basic principles and applications

    OpenAIRE

    Ella, Victor B.

    2007-01-01

    This presentation defines the term, irrigation, as well as explains the common methods of irrigation in attempt to define the fundamental principles needed to wisely design an irrigation system. It outlines a typical drip irrigation set-up, and discusses management of an irrigation system, including water volume application suggestions. LTRA-5 (Agroforestry and Sustainable Vegetable Production)

  15. Basic principles of forest fuel reduction treatments

    Science.gov (United States)

    James K. Agee; Carl N. Skinner

    2005-01-01

    Successful fire exclusion in the 20th century has created severe fire problems across the West. Not every forest is at risk of uncharacteristically severe wildfire, but drier forests are in need of active management to mitigate fire hazard. We summarize a set of simple principles important to address in fuel reduction treatments: reduction of surface fuels, increasing...

  16. Basic principles of chemistry and physical chemistry

    International Nuclear Information System (INIS)

    Colmenares, C.A.

    1975-01-01

    A course designed to provide communications between the designer of a system such as a mechanical engineer or a physicist and the material scientist such as a chemist or chemical engineer is presented. The topics discussed are stoichiometric principles, behavior of ideal gases, vapor pressure, humidity and saturation, solubility of gases in liquids, diffusion of gases, chemical reaction kinetics, and application of concepts to compatibility problems. The appendix provides problems to be used in conjunction with TV lectures

  17. Basic principles for regulating nuclear activities

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1996-03-01

    The AECB has developed as its mission statement: `To ensure that the use of nuclear energy in Canada does not pose undue risk to health, safety, security and the environment`. This report proposes eleven qualitative principles for regulating nuclear activities whose achievement would satisfy the broad policy enunciated in the statement. They would further provide a basis for the specific regulatory requirements expressed by the AECB in its Regulations and other documents. They would thus represent a connecting link between the policy enunciated in the mission statement and the requirements. The proposed principles are largely concerned with how the allowable risk should be set for members of the public, for industry workers, for society as a whole, and for the environment. In making these recommendations the risks from normal operation of the licensed facility and those from a possible serious accident are considered separately. The distribution of risk between geographic communities and between generations is also addressed in the proposed principles. These are listed in the final section of the report. 23 refs.

  18. Basic principles for regulating nuclear activities

    International Nuclear Information System (INIS)

    1996-03-01

    The AECB has developed as its mission statement: 'To ensure that the use of nuclear energy in Canada does not pose undue risk to health, safety, security and the environment'. This report proposes eleven qualitative principles for regulating nuclear activities whose achievement would satisfy the broad policy enunciated in the statement. They would further provide a basis for the specific regulatory requirements expressed by the AECB in its Regulations and other documents. They would thus represent a connecting link between the policy enunciated in the mission statement and the requirements. The proposed principles are largely concerned with how the allowable risk should be set for members of the public, for industry workers, for society as a whole, and for the environment. In making these recommendations the risks from normal operation of the licensed facility and those from a possible serious accident are considered separately. The distribution of risk between geographic communities and between generations is also addressed in the proposed principles. These are listed in the final section of the report. 23 refs

  19. Basic principles of applied nuclear techniques

    International Nuclear Information System (INIS)

    Basson, J.K.

    1976-01-01

    The technological applications of radioactive isotopes and radiation in South Africa have grown steadily since the first consignment of man-made radioisotopes reached this country in 1948. By the end of 1975 there were 412 authorised non-medical organisations (327 industries) using hundreds of sealed sources as well as their fair share of the thousands of radioisotope consignments, annually either imported or produced locally (mainly for medical purposes). Consequently, it is necessary for South African technologists to understand the principles of radioactivity in order to appreciate the industrial applications of nuclear techniques [af

  20. Basic principles for occupational radiation monitoring

    International Nuclear Information System (INIS)

    1987-01-01

    This Safety Guide sets forth the objectives of an adequate strategy for monitoring internal and external radiation exposures of workers. It covers individual monitoring, and workplace monitoring to the extent required for assessment and control of individual radiation doses. The responsibilities of authorities for organizing the monitoring of radiation workers are discussed, and brief descriptions are given of the rules governing the implementation of monitoring methods. The general principles to be considered in selecting instrumentation and appropriate monitoring techniques are described, as well as calibrating techniques, methods of record keeping and related aspects

  1. Basic principles of diffusion-weighted imaging

    International Nuclear Information System (INIS)

    Bammer, Roland.

    2003-01-01

    In diffusion-weighted MRI (DWI), image contrast is determined by the random microscopic motion of water protons. During the last years, DWI has become an important modality in the diagnostic work-up of acute ischemia in the CNS. There are also a few promising reports about the application of DWI to other regions in the human body, such as the vertebral column or the abdomen. This manuscript provides an introduction into the basics of DWI and Diffusion Tensor imaging. The potential of various MR sequences in concert with diffusion preparation are discussed with respect to acquisition speed, spatial resolution, and sensitivity to bulk physiologic motion. More advanced diffusion measurement techniques, such as high angular resolution diffusion imaging, are also addressed

  2. Application of Basic Principles for Intervention

    International Nuclear Information System (INIS)

    Skanata, D.; Sinka, D.; Jurkovic, I.-A.

    1998-01-01

    The paper deals with the basic terms which are within scope of activities of the expert group for risk analysis and assessment of potential consequences of a nuclear accident. The expert group is a part of the Technical Support Center which is going to be established within a new croatian national emergency response plan as the Lead Technical Agency in case of a nuclear emergency. Terms such as protective measures (urgent and long-term), intervention levels (generic and operational), projected and avertable doses are considered. Potential accident in the Krsko NPP, as an example for implementation of mentioned terms, is analyzed and a rapid approach for assessment in pretective measures which would be taken is applied. It is emphasized that rapid methods are characterized by a high degree of uncertainty. It is concluded that the time factor remains the most critical one in the entire accident response process. In order to optimize the time necessary for decision making, implementation and surveillance of protective measures, a set of requirements are pointed out. (author)

  3. Description of basic mining legal principles.

    Science.gov (United States)

    Schmidt, Reinhard

    2014-01-01

    The Federal Mining Act manages access, via the system of mining concessions, to areas free for mining natural resources that do not belong to the surface property and deposits' owner. These cover especially important natural resources for the economy, including coal, ore, salt, crude oil and natural gas, and also terrestrial heat. For mining operations there exist, however, the same decrees for natural resources in the property of the surface owners, which are predominantly higher-value industrial minerals such as roofing slate, basalt, quartz sand, and clays for the fireproofing industry. In the case of mining laws, administrative procedures such as issuing mining concessions, approving operating plans, and issuing permits or licenses to explore according to water rights or the Federal Immission Control Act, those authorities and departments in whose remit the projects fall are dealt with by the Mining Authority. This means that the Mining Authority is the only state point of contact for the applicant, essentially an "all-in-one" service as it will itself instigate any further participation procedures required. The classic licensing procedure of mining is the operations plan procedure, whereby the operator submits an operating plan to the Mining Authority, which then examines it to ensure it fulfills mandatory legal safety objectives. If necessary these safety objectives can be met during licensing of the operating plans by stipulating additional requirements, Depending on the subject and validity period there are overall operating plans having the widest possible remit with comprehensive participation by the authorities and basic operating plans that form the basis for every mining works. There are also special operating plans, which owing to the dynamics of mining, resolve matters that suddenly become necessary or when the basic operating plans as originally conceived were not relevant. The closing-down operating plan is the designated tool for closing down

  4. Basic principles governing the design of magnetic switches

    International Nuclear Information System (INIS)

    Birx, D.L.; Lauer, E.J.; Reginato, L.L.; Schmidt, J.; Smith, M.

    1980-01-01

    The idea of using saturable reactors as the basis of high power pulse generators is not a new concept, but there have been few recent applications of this technology. Here the principle of magnetic pulse generation is briefly described and some of the basic guidelines used to design these circuits are discussed. A demonstration of the principles by a small scale pulse amplifier is presented, and finally there is an extrapolation to a large scale system

  5. Squids: principles and basic applications in experimental physics

    International Nuclear Information System (INIS)

    Ocio, M.

    1990-01-01

    The basic principles and the description of the technical aspects of SQUIDs (Superconducting Quantum Interference Devices) are described. The applications of SQUIDs in experimental researches and low temperature physics experiments are given. The concepts of fluxoid quantization in a superconductor and Josephson tunnelling are reviewed. The principles, the operation, the noise and the different configurations of r.f. and direct current bias SQUIDs are summarized. The principal characteristics of several SQUIDs are reported

  6. Inpatient preanalytic process improvements.

    Science.gov (United States)

    Wagar, Elizabeth A; Phipps, Ron; Del Guidice, Robert; Middleton, Lavinia P; Bingham, John; Prejean, Cheryl; Johnson-Hamilton, Martha; Philip, Pheba; Le, Ngoc Han; Muses, Waheed

    2013-12-01

    Phlebotomy services are a common target for preanalytic improvements. Many new, quality engineering tools have recently been applied in clinical laboratories. However, data on relatively few projects have been published. This example describes a complete application of current, quality engineering tools to improve preanalytic phlebotomy services. To decrease the response time in the preanalytic inpatient laboratory by 25%, to reduce the number of incident reports related to preanalytic phlebotomy, and to make systematic process changes that satisfied the stakeholders. The Department of Laboratory Medicine, General Services Section, at the University of Texas MD Anderson Cancer Center (Houston) is responsible for inpatient phlebotomy in a 24-hour operation, which serves 689 inpatient beds. The study director was project director of the Division of Pathology and Laboratory Medicine's Quality Improvement Section and was assisted by 2 quality technologists and an industrial engineer from MD Anderson Office of Performance Improvement. After implementing each solution, using well-recognized, quality tools and metrics, the response time for blood collection decreased by 23%, which was close to meeting the original responsiveness goal of 25%. The response time between collection and arrival in the laboratory decreased by 8%. Applicable laboratory-related incident reports were reduced by 43%. Comprehensive application of quality tools, such as statistical control charts, Pareto diagrams, value-stream maps, process failure modes and effects analyses, fishbone diagrams, solution prioritization matrices, and customer satisfaction surveys can significantly improve preset goals for inpatient phlebotomy.

  7. Declaration of Basic Principles for the Treatment of Prisoners.

    Science.gov (United States)

    Cosman, J. W.

    1989-01-01

    Education does not flourish in prisons because of prevailing notions about the punitive and retributive purposes of prisons. The United Nations is considering a Declaration of Basic Principles for the Treatment of Prisoners that is intended to bring education to the forefront of criminal justice policy. (SK)

  8. Basic economic principles of road pricing: From theory to applications

    NARCIS (Netherlands)

    Rouwendal, J.; Verhoef, E.T.

    2006-01-01

    This paper presents, a non-technical introduction to the economic principles relevant for transport pricing design and analysis. We provide the basic rationale behind pricing of externalities, discuss why simple Pigouvian tax rules that equate charges to marginal external costs are not optimal in

  9. Basic principles and criteria on radioactive waste disposal sites

    International Nuclear Information System (INIS)

    Dlouhy, Z.; Kropikova, S.

    1980-01-01

    The basic principles are stated of radiation protection of the workers at radioactive waste disposal facilities, which must be observed in the choice of radioactive waste disposal sites. The emergency programme, the operating regulations and the safety report are specified. Workplace safety regulations are cited. (author)

  10. Designing User-Computer Dialogues: Basic Principles and Guidelines.

    Science.gov (United States)

    Harrell, Thomas H.

    This discussion of the design of computerized psychological assessment or testing instruments stresses the importance of the well-designed computer-user interface. The principles underlying the three main functional elements of computer-user dialogue--data entry, data display, and sequential control--are discussed, and basic guidelines derived…

  11. Clinical teachers' tacit knowledge of basic pedagogic principles.

    Science.gov (United States)

    McLeod, P J; Meagher, T; Steinert, Y; Schuwirth, L; McLeod, A H

    2004-02-01

    Academic faculty members in medical schools rarely receive formal instruction in basic pedagogic principles; nevertheless many develop into competent teachers. Perhaps they acquire tacit knowledge of these principles with teaching experience. This study was designed to assess clinical teachers' tacit knowledge of basic pedagogic principles and concepts. The authors developed a multiple-choice question (MCQ) exam based on 20 pedagogic principles judged by a panel of education experts to be important for clinical teaching. Three groups of clinician-educators sat the test: (1) clinicians with advanced education training and experience; (2) internal medicine specialists; (3) surgical specialists. All four groups of clinicians-educators passed the test, indicating that they possess a reasonable tacit knowledge of basic pedagogic principles. Those with advanced education training performed much better than members of the other two groups while specialists and residents working in teaching hospitals outperformed specialists from non-teaching hospitals. It is possible that converting this tacit knowledge to explicit knowledge may improve individual teaching effectiveness.

  12. [Etiopathogenesis of diarrhea and basic principles of diagnosis and therapy].

    Science.gov (United States)

    Ehrmann, J

    2002-06-01

    Acute diarrhoea is worldwide the second most frequent disease after acute inflammations of the airways. Chronic diarrhoea is a less frequent disease, nevertheless the GP, specialist in internal medicine or gastroenterologist encounters it very frequently. For correct understanding of basic diagnostic and therapeutic principles of diarrhoea knowledge of its etiopathogenesis is necessary. In the submitted review the author mentions the functions of the small and large intestine and their part in the development of diarrhoea. He gives also the definition and classification of diarrhoea. The author presents the basic characteristics of osmotic, secretory, inflammatory, motor diarrhoea and diarrhoea associated with increased intestinal filtration. The basic diagnostic and therapeutic principles are in the last part of the review which has an educational character.

  13. Power electronics basics operating principles, design, formulas, and applications

    CERN Document Server

    Rozanov, Yuriy; Chaplygin, Evgeny; Voronin, Pavel

    2015-01-01

    Power Electronics Basics: Operating Principles, Design, Formulas, and Applications provides fundamental knowledge for the analysis and design of modern power electronic devices. This concise and user-friendly resource:Explains the basic concepts and most important terms of power electronicsDescribes the power assemblies, control, and passive components of semiconductor power switchesCovers the control of power electronic devices, from mathematical modeling to the analysis of the electrical processesAddresses pulse-width modulation, power quality control, and multilevel, modular, and multicell

  14. Basic Theoretical Principles Pertaining to Thermal Protection of Oil Transformer

    Directory of Open Access Journals (Sweden)

    O. G. Shirokov

    2008-01-01

    Full Text Available The paper contains formulation of basic theoretical principles pertaining to thermal protection of an oil transformer in accordance with classical theory of relay protection and theory of diagnostics with the purpose of unification of terminological and analytical information which is presently available in respect of this problem. Classification of abnormal thermal modes of an oil transformer and also algorithms and methods for operation of diagnostic thermal protection of a transformer have been proposed.

  15. Some Basic Principles of Fish processing in Nigeria

    OpenAIRE

    J.F.N. Abowei; C.C. Tawari

    2011-01-01

    Some basic principles offish processing in Nigeria is reviewed to provide information for fish culturist to effectively manage the processing of their products. Processing of fish into forms for human consumption or suitable to be used as a supplement in animal food has been neglected in fish culture practices. This may be due to the high technology required in some of the processes and the fact that those involved in actual fish production are ignorant of the different processing methods. In...

  16. THE BASIC PRINCIPLES OF RESEARCH IN NEUROEDUCATION STUDIES

    OpenAIRE

    Ali Nouri

    2016-01-01

    The present paper assembles contributions from the areas of education, psychology, cognitive science, and of course, neuroeducation itself to introduce the basic principles of research in the field of neuroeducation studies. It is particularly important, as such it is a useful way to justify researchers about what neuroeducation as a specific domain do that no other field can do as well or cannot do at all. Based on the literature reviewed, neuroeducational research can be understood as an inte...

  17. Basic safety principles of KLT-40C reactor plants

    International Nuclear Information System (INIS)

    Beliaev, V.; Polunichev, V.

    2000-01-01

    The KLT-40 NSSS has been developed for a floating power block of a nuclear heat and power station on the basis of ice-breaker-type NSSS (Nuclear Steam Supply System) with application of shipbuilding technologies. Basic reactor plant components are pressurised water reactor, once-through coil-type steam generator, primary coolant pump, emergency protection rod drive mechanisms of compensate group-electromechanical type. Basic RP components are incorporated in a compact steam generating block which is arranged within metal-water shielding tank's caissons. Domestic regulatory documents on safety were used for the NSSS design. IAEA recommendations were also taken into account. Implementation of basic safety principles adopted presently for nuclear power allowed application of the KLT-40C plant for a floating power unit of a nuclear co-generation station. (author)

  18. Basic principles for intervention after a nuclear or radiological emergency

    International Nuclear Information System (INIS)

    Per Hedemann Jensen

    1996-01-01

    The current status of internationally agreed principles for intervention after a nuclear accident or radiological emergency and the international development of intervention guidance since the Chernobyl accident are reviewed. The experience gained after the Chernobyl accident indicates that the international advice on intervention existing at the time of the Chernobyl accident was not fully understood by decision makers neither in Western Europe nor in the former USSR and that the guidance failed to address adequately the difficult social problems which can arise after a serious nuclear accident. The radiation protection philosophy of today distinguishes between practices and interventions. The radiological protection system of intervention includes justification of the protective action and optimization of the level of protection achieved by that action. Dose limits do not apply in intervention situations. The inputs to justification and optimization studies include factors that are related to radiological protection, whereas the final decisions on introduction of countermeasures would also depend on other factors. The basic principles for intervention as recommended by international organisations are discussed in detail and the application of the principles on a generic basis is illustrated for long-term protective actions. The concepts of intervention level, operational intervention level and action level are presented and the relation between these quantities is illustrated. The numerical guidance on intervention in a nuclear accident or radiological emergency or a chronic exposure situation given by ICRP, IAEA and in the Basic Safety Standards is presented. (author)

  19. Data presentation in the WWER-440 Basic Principle Simulator

    International Nuclear Information System (INIS)

    Bota, J.; Gossanyi, A.; Vegh, E.

    1986-09-01

    At the Central Research Institute for Physics, Budapest, Hungary, a Basic Principle Simulator for WWER-440 nuclear power plants is under development. The input/output subsystem of the simulator is described. This subsystem is a control desk-shaped special periphery representing each main parameter of the model. During development special attention was paid to the hardware support of the controllers as this part of the model is regarded as most time consuming. A short summary on the hardware configuration of the simulator is also presented. (author)

  20. Design and construction of a basic principle simulator: an experiment

    International Nuclear Information System (INIS)

    Fernandez, O.; Galdoz, E.; Flury, C.; Fontanini, H.; Maciel, F.; Rovere, L.; Carpio, R.

    1992-01-01

    This paper describes activities developed over design and building of a Basic Principle Simulator for nuclear power plants. This simulator has been developed in Process Control Division of Bariloche Atomic Center, Argentina. This project was sponsored jointly by CNEA and Atomic Energy International Organization, through the United Nations Program for Development. The paper specially emphasizes aspects like: architecture design methodology of real time simulators; graphic environment and interfaces design for users and instructor interaction, and for display information; test and validation of the used models; and human resources formation. Finally describes the actual implementation of the simulator to be used in Embalse Nuclear Power Plant. (author)

  1. Real-time Executive for a basic principle simulator

    International Nuclear Information System (INIS)

    Buerger, L.; Szegi, Zs.; Vegh, E.

    1987-09-01

    A basic principle simulator for WWER-440 type nuclear power plants is under development in the Central Research Institute for Physics, Budapest. So far the technological models of both to primary and secondary circuits are ready and this paper presents the Real-time Executive and the on-line operating environment which controls the simulator. This executive system contains eight programs and the detailed structure of the data base is presented. The control of the execution of the model programs, their timing and the error recoveries are also discussed. (author) 5 refs

  2. An Embalse nuclear power plant basic principles simulator

    International Nuclear Information System (INIS)

    Fernandez, Oscar; Galdoz, Erwin; Flury, Celso; Fontanini, Horacio

    1989-01-01

    A nuclear power plant basic principles simulator is a package of programs that numerically solve the dynamic equations of the simulated plant. This kind of tools is mainly used in the first step of training of operational personnel, to allow mental representation of physical phenomena governing the plant. They are also used for students or professional training, and experienced operators can also improve there performance under abnormal operation situations using the simulator. For the Embalse nuclear power plant, mainly the thermohydraulic behaviour, is simulated. The mathematical model was adapted from MANUVR, a code developed at the Electric Systems and Control Department of the National Atomic Energy Commission (CNEA). (Author) [es

  3. Basic principles of Hasse diagram technique in chemistry.

    Science.gov (United States)

    Brüggemann, Rainer; Voigt, Kristina

    2008-11-01

    Principles of partial order applied to ranking are explained. The Hasse diagram technique (HDT) is the application of partial order theory based on a data matrix. In this paper, HDT is introduced in a stepwise procedure, and some elementary theorems are exemplified. The focus is to show how the multivariate character of a data matrix is realized by HDT and in which cases one should apply other mathematical or statistical methods. Many simple examples illustrate the basic theoretical ideas. Finally, it is shown that HDT is a useful alternative for the evaluation of antifouling agents, which was originally performed by amoeba diagrams.

  4. Ion-Exchange Chromatography: Basic Principles and Application.

    Science.gov (United States)

    Cummins, Philip M; Rochfort, Keith D; O'Connor, Brendan F

    2017-01-01

    Ion-Exchange Chromatography (IEC) allows for the separation of ionizable molecules on the basis of differences in charge properties. Its large sample-handling capacity, broad applicability (particularly to proteins and enzymes), moderate cost, powerful resolving ability, and ease of scale-up and automation have led to it becoming one of the most versatile and widely used of all liquid chromatography (LC) techniques. In this chapter, we review the basic principles of IEC, as well as the broader criteria for selecting IEC conditions. By way of further illustration, we outline basic laboratory protocols to partially purify a soluble serine peptidase from bovine whole brain tissue, covering crude tissue extract preparation through to partial purification of the target enzyme using anion-exchange chromatography. Protocols for assaying total protein and enzyme activity in both pre- and post-IEC fractions are also described.

  5. Expert systems - basic principles and possible applications in nuclear energy

    International Nuclear Information System (INIS)

    Cain, D.G.; Schmidt, F.

    1987-01-01

    One of the primary goals of the application of mathematical methods and computational techniques in reactor physics is the effective and accurate solution of the neutron diffusion equation under various conditions. To reach this goal still requires much skill, experience, knowledge and imagination as can be seen from various contributions at this and other conferences. Experts are necessary. Will expert systems replace them. We shall discuss this question by describing the basic principles of problem solving by expert systems as compared to problem solving by mathematical and computational methods. From this we shall identify areas of possible applications of the new techniques in nuclear energy and develop some thoughts on present limitations. As a result we conclude that expert systems will not be able to replace experts as long as the experts use the systems to improve their own expertise

  6. PET/TAC: Basic principles, physiological variants and artifacts

    International Nuclear Information System (INIS)

    Jimenez V, A.M.

    2007-01-01

    This presentation is about the basic principles, physiologic variants and devices that work in the PET/TAC technique. Next the conclusions obtained in the same one are presented: For a correct evaluation of the PET/TAC images with FDG is necessary the knowledge of the image acquisition technique, as well as of the physiologic distribution of the FDG, variants of the normality, benign causes of captation and more frequent devices. The introduction of this hybrid procedure allows the correct anatomical localization and identification of the deposits of FDG largely avoiding false or doubtful interpretations, but it can also originate not specific devices existent in the conventional PET. The previous knowledge of the possible devices will make possible in certain cases its elimination and in other its identification avoiding incorrect interpretations. (Author)

  7. Basic principles of information technology organization in health care institutions.

    Science.gov (United States)

    Mitchell, J A

    1997-01-01

    This paper focuses on the basic principles of information technology (IT) organization within health sciences centers. The paper considers the placement of the leader of the IT effort within the health sciences administrative structure and the organization of the IT unit. A case study of the University of Missouri-Columbia Health Sciences Center demonstrates how a role-based organizational model for IT support can be effective for determining the boundary between centralized and decentralized organizations. The conclusions are that the IT leader needs to be positioned with other institutional leaders who are making strategic decisions, and that the internal IT structure needs to be a role-based hybrid of centralized and decentralized units. The IT leader needs to understand the mission of the organization and actively use change-management techniques.

  8. Basic principles of aggressive rehabilitation after anterior cruciate ligament reconstruction

    Directory of Open Access Journals (Sweden)

    Dubljanin-Raspopović Emilija

    2005-01-01

    Full Text Available Rehabilitation after ACL (anterior cruciate ligament reconstruction has drastically changed over the last decade, with the adoption of a more aggressive approach, right from the first day after surgery. Progress in the effectiveness of rehabilitation is based on improvements in operative techniques, as well as on the encouraging results of histological studies regarding graft healing. Despite a huge amount of research papers on this topic, a rehabilitation golden standard still has not been established, due to the complexity of this problem. In this review, we point out the basic principles of rehabilitation after arthroscopically assisted ACL reconstruction based on actual practices, as well as the importance of specific procedures for the prevention of complications during the postoperative period. The importance of range-of-motion exercises, early weight bearing, an appropriate gait scheme, patella mobilisation, pain and oedema control, as well as stretching and balance exercises is explained. The functional advantages of closed kinetic chain exercises, as well as their influence on the graft are also described, in comparison to open kinetic chain exercises. The fundamentals of returning to sports are revealed and the specific aspects of rehabilitation regarding graft choice are pointed out. While waiting for new clinical investigations, which are expected to enable the establishment of a rehabilitation golden standard, the outlined principles should be followed. The complexity of this injury requires treatment in highly specialised institutions.

  9. Basic humanitarian principles applicable to non-nationals.

    Science.gov (United States)

    Goodwin-gill, G S; Jenny, R K; Perruchoud, R

    1985-01-01

    This article examines the general status in international law of certain fundamental human rights to determine the minimum "no derogation" standards, and then surveys a number of formal agreements between stages governing migration matters, while examining some of the standard-setting work undertaken by the International Labor Organization (ILO) and other institutions. Article 13 of the Universal Declaration of Human Rights, proclaims the right of everyone to leave any country, including his or her own. The anti-discrimination provision is widely drawn and includes national or social origin, birth, or other status. Non-discrimination is frequently the core issue in migration matters; it offers the basis for a principles approach to questions involving non-nationals and their methodological analysis, as well as a standard for the progressive elaboration of institutions and practices. As a general rule, ILO conventions give particular importance to the principle of choice of methods by states for the implementation of standards, as well as to the principle of progressive implementation. Non-discrimination implies equality of opportunity in the work field, inremuneration, job opportunity, trade union rights and benefits, social security, taxation, medical treatment, and accommodation; basic legal guarantees are also matters of concern to migrant workers, including termination of employment, non-renewal of work permits, and expulsion. The generality of human rights is due not because the individual is or is not a member of a partucular group, and claims to such rights are not determinable according to membership, but according to the character of the right in question. The individualized aspect of fundamental human rights requires a case-by-case consideration of claims, and the recognition that to all persons now certain special duties are owed.

  10. Introduction to basic immunological methods : Generalities, Principles, Protocols and Variants of basic protocols

    International Nuclear Information System (INIS)

    Mejri, Naceur

    2013-01-01

    This manuscript is dedicated to student of biological sciences. It provides the information necessary to perform practical works, the most commonly used in immunology. During my doctoral and post-doctoral periods, panoply of methods was employed in diverse subjects in my research. Technical means used in my investigations were diverse enough that i could extract a set of techniques that cover most the basic immunological methods. Each chapter of this manuscript contains a fairly complete description of immunological methods. In each topic the basic protocol and its variants were preceded by background information provided in paragraphs concerning the principle and generalities. The emphasis is placed on describing situations in which each method and its variants were used. These basic immunological methods are useful for students and even researchers studying the immune system of human, nice and other species. Different subjects showed not only detailed protocols but also photos or/and shemas used as support to illustrate some knowledge or practical knowledge. I hope that students will find this manual interesting, easy to use contains necessary information to acquire skills in immunological practice. (Author)

  11. Gyroscope precession in special and general relativity from basic principles

    Science.gov (United States)

    Jonsson, Rickard M.

    2007-05-01

    In special relativity a gyroscope that is suspended in a torque-free manner will precess as it is moved along a curved path relative to an inertial frame S. We explain this effect, which is known as Thomas precession, by considering a real grid that moves along with the gyroscope, and that by definition is not rotating as observed from its own momentary inertial rest frame. From the basic properties of the Lorentz transformation we deduce how the form and rotation of the grid (and hence the gyroscope) will evolve relative to S. As an intermediate step we consider how the grid would appear if it were not length contracted along the direction of motion. We show that the uncontracted grid obeys a simple law of rotation. This law simplifies the analysis of spin precession compared to more traditional approaches based on Fermi transport. We also consider gyroscope precession relative to an accelerated reference frame and show that there are extra precession effects that can be explained in a way analogous to the Thomas precession. Although fully relativistically correct, the entire analysis is carried out using three-vectors. By using the equivalence principle the formalism can also be applied to static spacetimes in general relativity. As an example, we calculate the precession of a gyroscope orbiting a static black hole.

  12. Basic Principles of Interpersonal Social Rhythm Therapy in Bipolar Disorder

    Directory of Open Access Journals (Sweden)

    Gokben Hizli Sayar

    2014-08-01

    Full Text Available Interpersonal Social Rhythm Therapy is a psychotherapy modality that helps the patient recognize the relationship between disruptions in social rhythms and the onset of previous episodes of psychiatric disorders. It uses psychoeducation and behavioral techniques to maintain social rhythm and sleep/wake regularity. It is closely related to and ldquo;social zeitgeber theory and rdquo; that emphasizes the importance that social rhythm regularity may play in synchronization of circadian rhythms in individuals with or at risk for bipolar spectrum disorders. Interpersonal and social rhythm therapy have been shown to stabilize social rhythms and enhance course and outcome in bipolar disorder. This review focuses on the theoretical principles and the basic steps of interpersonal and social rhythm therapy as a psychotherapy approach in bipolar disorder. PubMed, Scopus, Google Scholar databases were searched without temporal restriction. Search terms included interpersonal social rhythm therapy, bipolar, mood disorders. Abstracts were reviewed for relevance, and randomized controlled trials of interpersonal and social rhythm therapy in bipolar disorder selected. These researches also summarized on the final part of this review. [Psikiyatride Guncel Yaklasimlar - Current Approaches in Psychiatry 2014; 6(4.000: 438-446

  13. THE BASIC PRINCIPLES OF TREATMENT OF ANKYLOSING SPONDYLITIS (BECHTEREW'S DISEASE

    Directory of Open Access Journals (Sweden)

    Shandor Fedorovich Erdes

    2013-01-01

    Full Text Available The history of treatment of ankylosing spondylitis (AS and the reasons behind the development of modern approaches to its therapy are briefly described. The main tasks and principles of non-pharmacological and drug therapies for ASare briefly reported. The need for therapeutic physical training is emphasized, since its role in maintaining the functional status in this group of patients has been proved. The lecture casts doubt on effectiveness of physiotherapy andother physical techniques because of the poor evidence base. The lecture emphasizes the disease-modifying role of nonsteroidal anti-inflammatory drugs and requirement to receive them constantly for patients with Bechterew's disease. Meanwhile, the basic anti-inflammatory drugs in patients with this disease are reported to play an auxiliary role and to have no effect on disease progression. The pronounced clinical effectiveness of all tumor necrosis factor α inhibitors is emphasized; certain differences in their therapeutical effect are described. The problems of surgical treatment, which deal with correction of disease complications or undesirable effects, are discussed.

  14. BASIC PRINCIPLES OF TRANSPORT TOURISM DEVELOPMENT IN UKRAINE

    Directory of Open Access Journals (Sweden)

    L. V. Martsenyuk

    2013-08-01

    Full Text Available Purpose. Undeveloped infrastructure and the system of public and tourist transport services prevent boom and spread of tourism glory of the country. Therefore, the development of tourist infrastructure and transport communication routes is a priority task. Methodology. Article methodology is based on the use of consequent methodological technique. Findings. Author analyzed the situation of tourism industry in Ukraine, set the basic principles for the tourism development and its priorities. The article contains the author's point of view on the fact that the tourism industry is of paramount importance to the state economics, and the development of this sector of public life should be a priority task for the near future. Originality. According to the author, the development of the inbound tourism is more reasonable, because it provides additional workplaces and exchange earnings. The author insists that the raise of quality level of domestic tourist services to the European standards would accelerate the development of Ukrainian tourism and would attract more holidaymakers from Ukraine and neighbouring countries. Practical value. The rational measures, which were taken regarding the proposed directions for the tourism development, can improve competitiveness of the Ukrainian tourist industry on the European tourist market.

  15. The notion and basic principles of restorative justice

    Directory of Open Access Journals (Sweden)

    Ćopić Sanja

    2007-01-01

    Full Text Available One of the most important achievements of the contemporary criminal justice system and criminal policy is development of the concept of restorative justice. Contemporary concept of restorative justice was developed in 1970s on the basis of the criticism of the traditional criminal law and criminal justice system. Since that time, it has been developing through different programs in many countries. Reform of the criminal justice system in Serbia staring from 2002 went into direction of entering elements of restorative justice into existing criminal justice system. In that sense, development of restorative justice is still at the beginning in our country. However, it can be noticed that there is a low level of awareness on the nature and importance of restorative forms of response to crime among our professionals, as well as a lack of understanding of the concept itself. Due to that, the aim of the paper is to enable better understanding of restorative concept in general through defining restorative justice and basic principles it relies on. That may put a basis for further recognition of restorative elements in our criminal justice system, which may provide adequate implementation of relevant provisions of restorative character in practice. .

  16. Basic principles, contents, and benefits of an integrated management system

    International Nuclear Information System (INIS)

    Schwarzin, Juergen

    2010-01-01

    The basic principles and contents of an integrated management system are presented. The report focuses on the benefits and the experience accumulated in using an integrated management system. Integrated management systems are characterized by 2 features in particular: - On the one hand, by a system holistically controlling and describing all processes within a company which are necessary to achieve the company policy and company goals as defined. - On the other hand, it combines in one integrated management system various different aspects (such as quality, environmental protection, and safety) and the resulting requirements. Successful implementation of an integrated management system requires a clear commitment by company management to the integrated management system serving as a management tool. Implementation must be assigned the appropriate importance in the company. It must not be viewed as an instrument preserving 'status quo.' Instead, it must be seen as a tool for long-term improvement of the company. Application of the integrated management system minimizes the probability of occurrence of events, but is not able to reduce it to zero. (orig.)

  17. THE BASIC PRINCIPLES OF RESEARCH IN NEUROEDUCATION STUDIES

    Directory of Open Access Journals (Sweden)

    Ali Nouri

    2016-06-01

    Full Text Available The present paper assembles contributions from the areas of education, psychology, cognitive science, and of course, neuroeducation itself to introduce the basic principles of research in the field of neuroeducation studies. It is particularly important, as such it is a useful way to justify researchers about what neuroeducation as a specific domain do that no other field can do as well or cannot do at all. Based on the literature reviewed, neuroeducational research can be understood as an interdisciplinary endeavor to develop an insightful understanding and holistic picture of problems related to learning and education. It thus epistemologically is based on an integrated methodological pluralism paradigm. This requires researchers to understand multiple methods and methodologies and employ as they formulate their own research projects. Researchers have a critical role to play in providing systematic evidence and conclusions that are scientifically valid and reliable and educationally relevant and usable. One significant implication of this argument is the need to strengthen the quality of the research component in graduate programs of the field and train interested researchers in the identification and formulation of relevant research questions.

  18. Basic Modelling principles and Validation of Software for Prediction of Collision Damage

    DEFF Research Database (Denmark)

    Simonsen, Bo Cerup

    2000-01-01

    This report describes basic modelling principles, the theoretical background and validation examples for the collision damage prediction module in the ISESO stand-alone software.......This report describes basic modelling principles, the theoretical background and validation examples for the collision damage prediction module in the ISESO stand-alone software....

  19. Ethics of Plant Breeding: The IFOAM Basic Principles as a Guide for the Evolution of Organic Plant Breeding

    NARCIS (Netherlands)

    Lammerts Van Bueren, E.

    2010-01-01

    The basic values of organic agriculture is laid down in the IFOAM four basic principles: the principle of health, the principle of ecology, the principle of fairness and the principle of care. These principles and the consequences and challenges for the further development of organic plant breeding

  20. The Cyclical Relationship Approach in Teaching Basic Accounting Principles.

    Science.gov (United States)

    Golen, Steven

    1981-01-01

    Shows how teachers can provide a more meaningful presentation of various accounting principles by illustrating them through a cyclical relationship approach. Thus, the students see the entire accounting relationship as a result of doing business. (CT)

  1. EVALUATION OF STREET FURNITURE ACCORDING TO BASIC DESIGN PRINCIPLES

    OpenAIRE

    GHORAB, Peyman; YÜCEL CAYMAZ, Gökçen Firdevs

    2014-01-01

    In the urban context, it is important to create more comfortable and livable environments with proper planning, design and application. Because aesthetic considerations are of more importance today, designing urban furniture to give a more beautiful appearance to cities is of high priority; designers and those working in related disciplines must be careful to observe these principles throughout the design process. This paper describes research conducted to review the aesthetic principles invo...

  2. Using General Semantics Principles in the Basic News Reporting Classroom.

    Science.gov (United States)

    Russell, Charles G.; Many, Paul

    1993-01-01

    Shows how certain principles of the study of semantics might be employed in the journalism curriculum as a means of enhancing student reporters' understanding that their perceptions are limited and subject to distortion. Provides a model for explaining these limitations. (HB)

  3. Basic principles of medical aid in cases of radiation accidents

    International Nuclear Information System (INIS)

    Andreev, E.; Mikhajlov, M.A.; Bliznakov, V.

    1979-01-01

    A model scheme has been presented of medical aid organization in emergency cases of irradiation. The tasks of medical service have been pointed out in connection with evacuation stages, bulk of medical aid depending on the natur of radiation damages, first aid and some general principles of radiation sickness treatment. (author)

  4. Assessment Of Co60 Industrial Irradiators According To Basic Design Principles

    Directory of Open Access Journals (Sweden)

    El-Sayed Mohamed El Refaie

    2017-04-01

    Full Text Available Ensuring safe and easy operation providing relative uniform dose in the product and maximizing radiation utilization are the basic design principles for each Co60 industrial irradiator to maintain radiation safety. The study shows an assessment for four industrial irradiators to determine which active results were been maintained by using basic design principles. Different designs elements of the chosen irradiators have been illustrated and studied. The study shows that IRASM and ROBO industrial irradiators satisfy all basic design principles. IAEA-NR3772 irradiator maintains only two of the three basic design principles due to rotating door. Brevion irradiator satisfies only the principle of relative uniform radiation dose in product. Without affecting radiation safety this study proposes a new design of the irradiator to maximize energy utilization by adding a new track for low density products and also a static irradiation for cultural heritage beside the main track of high density products.

  5. Progress in basic principles of limitation in radiation protection

    International Nuclear Information System (INIS)

    Ramzaev, P.V.; Tarasov, S.I.; Troitskaya, M.N.; Ermolaeva, A.P.

    1977-01-01

    For purposes of limitation of harmful factors, e.g. radiation, it is proposed to divide all countless numbers of biological effects into three groups: 1) social important effects (ultimate and effects); 2) intermediate effects (different diseases etc.), which are connected with and controlled by the first group; 3) pure biological effects, importance of which is not known. To determine the first group effects there are identified four indices describing all significant sides of human life: time of life, life-time integral of mental and physical capacity for work, aesthetical satisfaction from organism itself, reproduction of descendants. They reflect the main social and individual interests related to functioning of organism. On the base of weighing these indices it is suggested the united general index of health in form of time of a full life. The united index can be used for different principles of limitation (based on threshold, acceptable risk, maximum benefit). To realize the principle of maximum public benefit as ideal principle in the future limitation all benefit and detriment from utilization of harmful sources must be expressed in the united index of health (instead of money), which is the greatest value of individual and society. Authors suggest to standartize ionizing radiation on the general methodological approaches that were acceptable to non-ionizing factors too

  6. Welding As Science: Applying Basic Engineering Principles to the Discipline

    Science.gov (United States)

    Nunes, A. C., Jr.

    2010-01-01

    This Technical Memorandum provides sample problems illustrating ways in which basic engineering science has been applied to the discipline of welding. Perhaps inferences may be drawn regarding optimal approaches to particular welding problems, as well as for the optimal education for welding engineers. Perhaps also some readers may be attracted to the science(s) of welding and may make worthwhile contributions to the discipline.

  7. Basic principles of RF superconductivity and superconducting cavities

    OpenAIRE

    Schmüser, P

    2006-01-01

    The basics of superconductivity are outlined with special emphasis on the features which are relevant for the application of superconductors in radio frequency cavities for particle acceleration. For a cylindrical resonator (“pill box cavity”) the electromagnetic field in the cavity and important parameters such as resonance frequency, quality factor and shunt impedance are calculated analytically. The design and performance of practical cavities is shortly addressed.

  8. [Sampling in qualitative research: basic principles and some controversies].

    Science.gov (United States)

    Martínez-Salgado, Carolina

    2012-03-01

    This paper presents the rationale for the choice of participants in qualitative research in contrast with that of probability sampling principles in epidemiological research. For a better understanding of the differences, concepts of nomothetic and ideographic generalizability, as well as those of transferability and reflexivity, are proposed, Fundamentals of the main types of sampling commonly used in qualitative research, and the meaning of the concept of saturation are mentioned. Finally, some reflections on the controversies that have arisen in recent years on various paradigmatic perspectives from which to conduct qualitative research, their possibilities of combination with epidemiological research, and some implications for the study of health issues are presented.

  9. Ions in solution basic principles of chemical interactions

    CERN Document Server

    Burgess, J

    1999-01-01

    This outline of the principles and chemical interactions in inorganic solution chemistry delivers a course module in an area of considerable complexity. Problems with solutions and tutorial hints to test comprehension have been added as a feature to check readers' understanding and assist self-study. Exercises and projects are also provided to help readers deepen and extend their knowledge and understanding. Inorganic solution chemistry is treated thoroughly Emphasis is placed upon NMR, UV-VIS, IR Raman spectroscopy, X-ray diffraction, and such topics as acid-base behaviour, stability constants and kinetics.

  10. Development and application of the basic principles of radiation protection

    International Nuclear Information System (INIS)

    Huyskens, C.J.

    1986-01-01

    The basic philosophy of radiation protection, i.e. to prevent non-stochastic effects and to reduce stochastic effects to an acceptable level, is realized in the internationally accepted three-stage system of dose reduction, viz. justification, ALARA and dose limits. Historical development of this three-stage system is described and the three components are worked out further. The application and applicability of the system is considered for radiation sources and accidents. Attention is also given to regulations, national policy and practice of radiation protection. (G.J.P.)

  11. 41 CFR 102-85.25 - What is the basic principle governing OAs?

    Science.gov (United States)

    2010-07-01

    ... Property Management Regulations System (Continued) FEDERAL MANAGEMENT REGULATION REAL PROPERTY 85-PRICING POLICY FOR OCCUPANCY IN GSA SPACE Pricing Policy-General § 102-85.25 What is the basic principle...

  12. Basic principles of magnetic resonance imaging in cerebral ischemia and initial clinical experience

    International Nuclear Information System (INIS)

    Brant-Zawadzki, M.; Solomon, M.; Newton, T.H.; Weinstein, P.; Schmidley, J.; Norman, D.

    1985-01-01

    The basic principles of magnetic resonance imaging are described and their use in the investigation of cerebral ischemia outlined. A brief account is given of the clinical results of investigation to date

  13. Differences between application of some basic principles of quantum mechanics on atomic and mesoscopic levels

    OpenAIRE

    Nikulov, Alexey

    2005-01-01

    Formalism of the quantum mechanics developed for microscopic (atomic) level comes into collision with some logical difficulties on mesoscopic level. Some fundamental differences between application of its basic principles on microscopic and mesoscopic levels are accentuated.

  14. Basic principles and applications of atmospheric-pressure discharge plasmas

    International Nuclear Information System (INIS)

    Becker, K.H.

    2002-01-01

    The principles that govern the generation and maintenance of atmospheric - pressure discharge plasmas are summarized. The properties and operating parameters of various types such as dielectric barrier discharge plasmas (DBDs), corona discharge plasmas (CDs), microhollow cathode discharge plasmas (MHCDs) , and dielectric capillary electrode discharge plasmas (CDEDs) are introduced. All of them are self sustained, non equilibrium gas discharges that can be operated at atmospheric pressure. CDs and DBDDs represent very similar types of discharges, while DBDs are characterized by insulating layers on one or both electrodes, CDs depend on inhomogeneous electric fields at least in some parts of the electrode configuration to restrict the primary ionization processes to a small fraction of the inter - electrode region. Their application to novel light sources in the ultraviolet (UV) and vacuum ultraviolet (VUV) spectral region is described. (nevyjel)

  15. [Basic principles and methodological considerations of health economic evaluations].

    Science.gov (United States)

    Loza, Cesar; Castillo-Portilla, Manuel; Rojas, José Luis; Huayanay, Leandro

    2011-01-01

    Health Economics is an essential instrument for health management, and economic evaluations can be considered as tools assisting the decision-making process for the allocation of resources in health. Currently, economic evaluations are increasingly being used worldwide, thus encouraging evidence-based decision-making and seeking efficient and rational alternatives within the framework of health services activities. In this review, we present an overview and define the basic types of economic evaluations, with emphasis on complete Economic Evaluations (EE). In addition, we review key concepts regarding the perspectives from which EE can be conducted, the types of costs that can be considered, the time horizon, discounting, assessment of uncertainty and decision rules. Finally, we describe concepts about the extrapolation and spread of economic evaluations in health.

  16. Bayesian inference in processing experimental data: principles and basic applications

    International Nuclear Information System (INIS)

    D'Agostini, G

    2003-01-01

    This paper introduces general ideas and some basic methods of the Bayesian probability theory applied to physics measurements. Our aim is to make the reader familiar, through examples rather than rigorous formalism, with concepts such as the following: model comparison (including the automatic Ockham's Razor filter provided by the Bayesian approach); parametric inference; quantification of the uncertainty about the value of physical quantities, also taking into account systematic effects; role of marginalization; posterior characterization; predictive distributions; hierarchical modelling and hyperparameters; Gaussian approximation of the posterior and recovery of conventional methods, especially maximum likelihood and chi-square fits under well-defined conditions; conjugate priors, transformation invariance and maximum entropy motivated priors; and Monte Carlo (MC) estimates of expectation, including a short introduction to Markov Chain MC methods

  17. Solid-state NMR basic principles and practice

    CERN Document Server

    Apperley, David C; Hodgkinson, Paul

    2014-01-01

    Nuclear Magnetic Resonance (NMR) has proved to be a uniquely powerful and versatile tool for analyzing and characterizing chemicals and materials of all kinds. This book focuses on the latest developments and applications for "solid-state" NMR, which has found new uses from archaeology to crystallography to biomaterials and pharmaceutical science research. The book will provide materials engineers, analytical chemists, and physicists, in and out of lab, a survey of the techniques and the essential tools of solid-state NMR, together with a practical guide on applications. In this concise introduction to the growing field of solid-state nuclear magnetic resonance spectroscopy The reader will find: * Basic NMR concepts for solids, including guidance on the spin-1/2 nuclei concept * Coverage of the quantum mechanics aspects of solid state NMR and an introduction to the concept of quadrupolar nuclei * An understanding relaxation, exchange and quantitation in NMR * An analysis and interpretation of NMR data, with e...

  18. Photoelectrochemical solar water splitting: From basic principles to advanced devices

    Directory of Open Access Journals (Sweden)

    Bandar Y.Alfaifi

    2018-02-01

    Full Text Available Photoelectrochemical water splitting (PEC offers a promising path for sustainable generation of hydrogen fuel. However, improving solar fuel water splitting efficiency facing tremendous challenges, due to the energy loss related to fast recombination of the photogenerated charge carriers, electrode degradation, as well as limited light harvesting. This review focuses on the brief introduction of basic fundamental of PEC water splitting and the concept of various types of water splitting approaches. Numerous engineering strategies for the investgating of the higher efficiency of the PEC, including charge separation, light harvesting, and co-catalysts doping, have been discussed. Moreover, recent remarkable progress and developments for PEC water splitting with some promising materials are discussed. Recent advanced applications of PEC are also reviewed. Finally, the review concludes with a summary and future outlook of this hot field.

  19. Optical properties of metallic nanoparticles basic principles and simulation

    CERN Document Server

    Trügler, Andreas

    2016-01-01

    This book introduces the fascinating world of plasmonics and physics at the nanoscale, with a focus on simulations and the theoretical aspects of optics and nanotechnology. A research field with numerous applications, plasmonics bridges the gap between the micrometer length scale of light and the secrets of the nanoworld. This is achieved by binding light to charge density oscillations of metallic nanostructures, so-called surface plasmons, which allow electromagnetic radiation to be focussed down to spots as small as a few nanometers. The book is a snapshot of recent and ongoing research and at the same time outlines our present understanding of the optical properties of metallic nanoparticles, ranging from the tunability of plasmonic resonances to the ultrafast dynamics of light-matter interaction. Beginning with a gentle introduction that highlights the basics of plasmonic interactions and plasmon imaging, the author then presents a suitable theoretical framework for the description of metallic nanostructu...

  20. Synchrotron radiation, a powerful tool in research and technological development. Basic principles

    International Nuclear Information System (INIS)

    Jimenez M, J.

    2001-01-01

    The basic principles of synchrotron radiation emission in electron accelerators are presented. The main characteristics of synchrotron radiation, together with the physical principles that describe its interaction with different materials are also discussed. Different areas in which the development of synchrotron radiation has made a major impact are given. (Author)

  1. Embarrassment as a key to understanding cultural differences. Basic principles of cultural analysis

    DEFF Research Database (Denmark)

    Bouchet, Dominique

    1995-01-01

    I introduce here the principles I use in my investigation of intercultural marketing and management. I explain how I discovered them, and show how they spring from a theoretical understanding of the dynamic of cultural differences. One of the basic methodological principles for my analysis...

  2. Basic principles of taxation of small-scale enterprises in the Russian Federation

    OpenAIRE

    Khodyreva Viktoriia Andreevna

    2015-01-01

    This research is devoted to general principles of taxation of small-scale enterprises. Development of small-scale enterprises is one of the most important lines of tax policy in Russian Federation. The development of principles of taxation is important while forming a strong state system of taxation. In this work basic principles and some specific are provided. This work is of great scientific interest to law students, graduates, teachers and other persons interested in law and particularly i...

  3. Basic principles and results of the German risk study

    International Nuclear Information System (INIS)

    Heuser, F.W.; Bayer, A.

    1980-01-01

    In June 1976 the Federal Ministry for Research and Technology had commissioned the Gesellschaft fuer Reaktorsicherheit to write the German Risk Study, the first part of which has now been completed after three years of work and has been publicized recently. The German Risk Study is an attempt to define the societal risk posed by accidents in nuclear power plants under conditions in Germany. For this purpose, the accident rates and the resultant health hazards were determined. By adopting most of the basic premises and methods of the American Rasmussen Study, the German study is to allow a comparison to be made with the results of that study. The calculations were based on 19 sites with a total of 25 nuclear generating units presently in operation, under construction or in the licensing procedure in the Federal Republic of Germany. The technical studies were conducted on a 1300 MW PWR as the representative example. The results show that the decisive contributions are made by uncontrolled minor loss-of-coolant accidents and by failures of power supply (emergency power case). Large loss-of-coolant accidents do not play a role. The study also shows the decisive safety function of the containment. (orig.) [de

  4. Ultra-high energy physics and standard basic principles

    Directory of Open Access Journals (Sweden)

    Gonzalez-Mestres Luis

    2014-04-01

    Full Text Available It has not yet been elucidated whether the observed flux suppression for ultra-high energy cosmic rays (UHECR at energies above ≃ 4 x 1019 eV is a signature of the Greisen-Zatsepin-Kuzmin (GZK cutoff or a consequence of other phenomena. In both cases, violations of the standard fundamental principles of Physics can be present and play a significant role. They can in particular modify cosmic-ray interactions, propagation or acceleration at very high energy. Thus, in a long-term program, UHECR data can hopefully be used to test relativity, quantum mechanics, energy and momentum conservation, vacuum properties... as well as the elementariness of standard particles. Data on cosmic rays at energies ≃ 1020 eV may also be sensitive to new physics generated well beyond Planck scale. A typical example is provided by the search for possible signatures of a Lorentz symmetry violation (LSV associated to a privileged local reference frame (the "vacuum rest frame", VRF. If a VRF exists, the internal structure of standard particles at ultra-high energy can undergo substantial modifications. Similarly, the conventional particle symmetries may cease to be valid at such energies instead of heading to a grand unification and the structure of vacuum may no longer be governed by standard quantum field theory. Then, the question whether the notion of Planck scale still makes sense clearly becomes relevant and the very grounds of Cosmology can undergo essential modifications. UHECR studies naturally interact with the interpretation of WMAP and Planck observations. Recent Planck data analyses tend to confirm the possible existence of a privileged space direction. If the observed phenomenon turns out to be a signature of the spinorial space-time (SST we suggested in 1996-97, then conventional Particle Physics may correspond to the local properties of standard matter at low enough energy and large enough distances. This would clearly strengthen the cosmological

  5. Principles of interaction of ionizing radiation with matter and basic radiation chemistry processes

    International Nuclear Information System (INIS)

    Santar, I.; Bednar, J.

    1976-01-01

    The basic principles are given of the interaction of ionizing radiation with matter and the main trends are pointed out in radiation chemistry. A brief characteristics is given of the basic radiation chemical processes in gases and in the condensed phase, namely in water and in organic substances. (B.S.)

  6. ECOLOGICAL LIFESTYLE, THE BASIC PRINCIPLES OF THE OPERATIONAL DEFINITION OF THE CONCEPT

    Directory of Open Access Journals (Sweden)

    Olesya Nikolaevna Tarasova

    2015-12-01

    Full Text Available Based on the analysis of approaches to the empirical study of the environmental aspects of lifestyle are presented the basic principles that distinguish ecological way of life off the set of environmental practices that need to be taken into account in empirical measurement, namely: systematicity, conceptuality, contextual conformity and processuality. The article provides a theoretical basis and operational definition as an ecological way of life in general and the principles that distinguish it from other concepts of environmental sociology.

  7. Oncology. Pt. 1. General part, epidemiology - pathogenesis - basic principles of therapy. 2. upd. ed.

    International Nuclear Information System (INIS)

    Hiddemann, Wolfgang; Bartram Claus R.

    2010-01-01

    The book Oncology is aimed to communicate the compiled knowledge on tumor development and cancer: fundamental knowledge base, practice related know-how for diagnostics and therapy. Part 1 includes the following chapters: epidemiology and pathogenesis, basic principles of diagnostics, basic principles of therapy, complication of malign growth, tumors in the gastrointestinal tract, female genital carcinomas, kidney and urinary tract carcinomas, respiratory tract and lung carcinomas, carcinomas in the head - neck area, bone and soft tissue carcinomas, pediatric tumors, hematological neoplasm, other carcinomas. The book can be used as reference for clinical work. [de

  8. Basic environmental principles for the promotion of clean and efficient energy

    International Nuclear Information System (INIS)

    Hanmer, R.; Connor-Lajambe, H.

    1994-01-01

    The purpose of this paper is to reiterate what might be considered basic principles for promoting clean and efficient energy. These principles have very important implications for the design of energy supply and transportation facilities, but they go far beyond that to unify such design with the design, use and maintenance of many other types of facilities and goods. These principles also affect the way we consider energy security in the context of sustainable development. In annex, this paper presents the recommendation of the Council, with a list of environmentally favourable energy options. (TEC). 2 refs., Annex

  9. The Basic Principles and Methods of the System Approach to Compression of Telemetry Data

    Science.gov (United States)

    Levenets, A. V.

    2018-01-01

    The task of data compressing of measurement data is still urgent for information-measurement systems. In paper the basic principles necessary for designing of highly effective systems of compression of telemetric information are offered. A basis of the offered principles is representation of a telemetric frame as whole information space where we can find of existing correlation. The methods of data transformation and compressing algorithms realizing the offered principles are described. The compression ratio for offered compression algorithm is about 1.8 times higher, than for a classic algorithm. Thus, results of a research of methods and algorithms showing their good perspectives.

  10. Doing it right – or are we? Basic principles in the acquisition, care of ...

    African Journals Online (AJOL)

    The photograph collection of the University Archives, University of KwaZulu-Natal (Pietermaritzburg Campus), is introduced as an example of a collection. Basic principles for the acquisition, storage, provision of access, and handling and use of photographs, are presented. The applicability of 'acceptable' American and ...

  11. Bulgarian National System for Ionization Radiation Control in the Biosphere. Three basic principles, main features and specific aspects of application

    International Nuclear Information System (INIS)

    Vasilev, G.

    1994-01-01

    Some problems concerned with the structure of the national system for radiation control and 'de facto' situation in Bulgaria are outlined. The following three basic principles of such a system are discussed: 1. Radioecological principle; 2. Regulatory principle. Maximum permissible contamination level and exposure; 3. Control principle. Local variations of natural radiation background. 1 tab., 12 refs

  12. Rethinking Teaching of Basic Principles of Economics from a Sustainability Perspective

    Directory of Open Access Journals (Sweden)

    Namrata Chindarkar

    2018-05-01

    Full Text Available In this paper, we posit that sustainability warrants explicit recognition in the teaching of basic principles of economics. The conventional exposition of conceptual and analytic frameworks in basic principles in almost all standard economics textbooks overlooks at least two basic flaws. The first of these concerns the collection of residual externalities that exist without being internalized in market transactions and hence fall outside the calculus of national income accounting. For example, not all energy resource prices capture the entirety of the damages inflicted on natural ecosystems. The cumulus of residual externalities threatens the feasibility of sustainability. The second flaw is the absence of sustainability as a necessary condition in the fundamental benchmark of perfect competition (PC. Sustainability, when explicitly introduced in the PC benchmark, results in significant changes to conceptual premises in economics. The most significant of such changes concerns the axiomatic differentiation between “goods” and “bads”.

  13. Preanalytical quality improvement: in quality we trust.

    Science.gov (United States)

    Lippi, Giuseppe; Becan-McBride, Kathleen; Behúlová, Darina; Bowen, Raffick A; Church, Stephen; Delanghe, Joris; Grankvist, Kjell; Kitchen, Steve; Nybo, Mads; Nauck, Matthias; Nikolac, Nora; Palicka, Vladimir; Plebani, Mario; Sandberg, Sverre; Simundic, Ana-Maria

    2013-01-01

    Total quality in laboratory medicine should be defined as the guarantee that each activity throughout the total testing process is correctly performed, providing valuable medical decision-making and effective patient care. In the past decades, a 10-fold reduction in the analytical error rate has been achieved thanks to improvements in both reliability and standardization of analytical techniques, reagents, and instrumentation. Notable advances in information technology, quality control and quality assurance methods have also assured a valuable contribution for reducing diagnostic errors. Nevertheless, several lines of evidence still suggest that most errors in laboratory diagnostics fall outside the analytical phase, and the pre- and postanalytical steps have been found to be much more vulnerable. This collective paper, which is the logical continuum of the former already published in this journal 2 years ago, provides additional contribution to risk management in the preanalytical phase and is a synopsis of the lectures of the 2nd European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)-Becton Dickinson (BD) European Conference on Preanalytical Phase meeting entitled "Preanalytical quality improvement: in quality we trust" (Zagreb, Croatia, 1-2 March 2013). The leading topics that will be discussed include quality indicators for preanalytical phase, phlebotomy practices for collection of blood gas analysis and pediatric samples, lipemia and blood collection tube interferences, preanalytical requirements of urinalysis, molecular biology hemostasis and platelet testing, as well as indications on best practices for safe blood collection. Auditing of the preanalytical phase by ISO assessors and external quality assessment for preanalytical phase are also discussed.

  14. Assessment of IAEA safety series no. 75-INSAG-3 - ''basic safety principles for nuclear power plants''

    International Nuclear Information System (INIS)

    1989-01-01

    The International Atomic Energy Agency Safety Series No. 75-INSAG--3, 'Basic Safety Principles for Nuclear Power Plants' is reviewed in the light of the Advisory Committee on Nuclear Safety reports ACNS--2, 'Safety Objectives for Nuclear Activities in Canada', and ACNS--4, 'Recommended General Safety Requirements for Nuclear Power Plants'. The INSAG safety objectives are consistent with the safety objectives stated in ACNS--2 but are less general, applying only to nuclear power plants. The INSAG safety principles are, in general, consistent with the requirements stated in ACNS--4 but put more emphasis on 'safety culture'. They give little attention to reactor plant effluents, waste management, or decommissioning. (fig., 5 refs.)

  15. Preanalytical quality in clinical chemistry laboratory.

    Science.gov (United States)

    Ahmad, M Imteyaz; Ramesh, K L; Kumar, Ravi

    2014-01-01

    Haemolysis is usually caused by inadequate specimen collection or preanalytical handling and is suggested to be a suitable indicator of preanalytical quality. We investigated the prevalence of detectable haemolysis in all routine venous blood samples in OPDs and IPDs to identify differences in preanalytical quality. Haemolysis index (HI) values were obtained from a Vitros 5,1 in the routine clinical chemistry laboratory for samples collected in the outpatient department (OPD) collection centres, a hospital, and inpatient departments (IPD). Haemolysis was defined as a HI > or = 15 (detection limit). Samples from the OPD with the highest prevalence of haemolysis were 6.1 times (95% confidence interval (CI) 4.0 - 9.2) more often haemolysed compared to the center with the lowest prevalence. Of the samples collected in primary health care, 10.4% were haemolysed compared to 31.1% in the IPDs (p = 0.001). A notable difference in haemolysed samples was found between the IPDs section staffed by emergency medicine physicians and the section staffed by primary health care physicians (34.8% vs. 11.3%, p = 0.001). The significant variation in haemolysis indices among the investigated units is likely to reflect varying preanalytical conditions. The HI is a valuable tool for estimation and follow-up of preanalytical quality in the health care laboratory.

  16. Basic Principles of Industrial Electric Power Network Computer Aided Design and Engineering

    Directory of Open Access Journals (Sweden)

    M. I. Fursanov

    2012-01-01

    Full Text Available A conceptual model for a computer aided design and engineering system has been developed in the paper. The paper presents basic automation process principles including a graphical representation   network and calculation results, convenient user interface, automatic mode calculation, selection of transformer rated power and cross-section area of wires. The developed algorithm and program make it possible to save time and improve quality of project implementation.

  17. UPDATING THE BASIC PRINCIPLES OF PROJECT EDUCATION TECHNOLOGY IN FUTURE MUSIC TEACHERS’ VOCAL AND CHORAL TRAINING

    Directory of Open Access Journals (Sweden)

    Liang Haiye

    2017-04-01

    Full Text Available The article is devoted to characterizing features of implementing project technology into future music teachers’ vocal and choral training. The analysis of scientific papers of outstanding scientists in philosophy, psychology, and art education, which deal with modern directions of using project technology, highlight its role in art education process. A methodological base is supported by considering contemporary scientific researches, in particular the theory and methodology of musical studies in accordance with forming students’ independence in the process of solving educational problems by means of project technology; developing principles of students’ professional training optimization on the basis of project activity; innovative development of future music teachers’ professional training that gives to the presented material novelty and presentable appearance. Studying future music teachers’ vocal and choral training as a process of constructing that has a special purpose of improving the quality descriptions of educational vocal and choral collective sound functioning, the author of the article discloses the basic principles of implementing project technology into future music teachers’ vocal and choral training. The author of the article pays the special attention to revealing specific features and maintenance of project technology in vocal and choral training of future leaders of child's art groups. An emphasis is made on the following basic factors that influence development of students’ creative individuality: constructing projects of their own becoming; setting aims, tasks, strategies and facilities of vocal and choral work; directing to the result; independent creative activity; presentation, reflection and correction of a project. On the basis of the obtained data the following principles of project technology are put forward in future music teachers’ vocal and choral training: principle of independence; principle of

  18. An assessment of adherence to basic ecological principles by payments for ecosystem service projects.

    Science.gov (United States)

    Prager, C M; Varga, A; Olmsted, P; Ingram, J C; Cattau, M; Freund, C; Wynn-Grant, R; Naeem, S

    2016-08-01

    Programs and projects employing payments for ecosystem service (PES) interventions achieve their objectives by linking buyers and sellers of ecosystem services. Although PES projects are popular conservation and development interventions, little is known about their adherence to basic ecological principles. We conducted a quantitative assessment of the degree to which a global set of PES projects adhered to four ecological principles that are basic scientific considerations for any project focused on ecosystem management: collection of baseline data, identification of threats to an ecosystem service, monitoring, and attention to ecosystem dynamics or the formation of an adaptive management plan. We evaluated 118 PES projects in three markets-biodiversity, carbon, and water-compiled using websites of major conservation organizations; ecology, economic, and climate-change databases; and three scholarly databases (ISI Web of Knowledge, Web of Science, and Google Scholar). To assess adherence to ecological principles, we constructed two scientific indices (one additive [ASI] and one multiplicative [MSI]) based on our four ecological criteria and analyzed index scores by relevant project characteristics (e.g., sector, buyer, seller). Carbon-sector projects had higher ASI values (P < 0.05) than water-sector projects and marginally higher ASI scores (P < 0.1) than biodiversity-sector projects, demonstrating their greater adherence to ecological principles. Projects financed by public-private partnerships had significantly higher ASI values than projects financed by governments (P < 0.05) and marginally higher ASI values than those funded by private entities (P < 0.1). We did not detect differences in adherence to ecological principles based on the inclusion of cobenefits, the spatial extent of a project, or the size of a project's budget. These findings suggest, at this critical phase in the rapid growth of PES projects, that fundamental ecological principles should be

  19. Zebrafish housing systems: a review of basic operating principles and considerations for design and functionality.

    Science.gov (United States)

    Lawrence, Christian; Mason, Timothy

    2012-01-01

    The strategies for housing zebrafish used in biomedical research have evolved considerably over the past three decades. To keep pace with the rapid expansion and development of the zebrafish model system, the field has generally moved from keeping fish at the level of aquarium hobbyist to that of industrialized, recirculating aquaculture. Numerous commercial system vendors now offer increasingly sophisticated housing systems based on design principles that maximize the number of animals that can be housed in a given space footprint, and they are thus able to support large and diverse research programs. This review is designed to provide managers, lab animal veterinarians, investigators, and other parties responsible for care and use of these animals with a comprehensive overview of the basic operating and design principles of zebrafish housing systems. This information can be used to help plan the construction of new facilities and/or the upgrade and maintenance of existing operations.

  20. Basic Principles and Utilization Possibilities’ of Ultrasonic Phased Array in Material Nondestructive Evaluation

    Directory of Open Access Journals (Sweden)

    Dagmar Faktorova

    2004-01-01

    Full Text Available The paper deals with the basic principles of operation and with the utilization possibilities of phased array (PA in materials nondestructive testing (NDT. The first part deals with description of PA arrangement modes, which enable to generate, focus and steer the ultrasonic beem. The second part deals with the description of electromagnetic acoustic transducer PA operation. The last part deals with the description of the utilization of PA in nondestructive testing of conductive materials and the advantages of PA utilization in inhomogeneous materials NDT.

  1. Computational anatomy based on whole body imaging basic principles of computer-assisted diagnosis and therapy

    CERN Document Server

    Masutani, Yoshitaka

    2017-01-01

    This book deals with computational anatomy, an emerging discipline recognized in medical science as a derivative of conventional anatomy. It is also a completely new research area on the boundaries of several sciences and technologies, such as medical imaging, computer vision, and applied mathematics. Computational Anatomy Based on Whole Body Imaging highlights the underlying principles, basic theories, and fundamental techniques in computational anatomy, which are derived from conventional anatomy, medical imaging, computer vision, and applied mathematics, in addition to various examples of applications in clinical data. The book will cover topics on the basics and applications of the new discipline. Drawing from areas in multidisciplinary fields, it provides comprehensive, integrated coverage of innovative approaches to computational anatomy. As well,Computational Anatomy Based on Whole Body Imaging serves as a valuable resource for researchers including graduate students in the field and a connection with ...

  2. Basic principles of test-negative design in evaluating influenza vaccine effectiveness.

    Science.gov (United States)

    Fukushima, Wakaba; Hirota, Yoshio

    2017-08-24

    Based on the unique characteristics of influenza, the concept of "monitoring" influenza vaccine effectiveness (VE) across the seasons using the same observational study design has been developed. In recent years, there has been a growing number of influenza VE reports using the test-negative design, which can minimize both misclassification of diseases and confounding by health care-seeking behavior. Although the test-negative designs offer considerable advantages, there are some concerns that widespread use of the test-negative design without knowledge of the basic principles of epidemiology could produce invalid findings. In this article, we briefly review the basic concepts of the test-negative design with respect to classic study design such as cohort studies or case-control studies. We also mention selection bias, which may be of concern in some countries where rapid diagnostic testing is frequently used in routine clinical practices, as in Japan. Copyright © 2017. Published by Elsevier Ltd.

  3. Basic principles on the safety evaluation of the HTGR hydrogen production system

    International Nuclear Information System (INIS)

    Ohashi, Kazutaka; Nishihara, Tetsuo; Tazawa, Yujiro; Tachibana, Yukio; Kunitomi, Kazuhiko

    2009-03-01

    As HTGR hydrogen production systems, such as HTTR-IS system or GTHTR300C currently being developed by Japan Atomic Energy Agency, consists of nuclear reactor and chemical plant, which are without a precedent in the world, safety design philosophy and regulatory framework should be newly developed. In this report, phenomena to be considered and events to be postulated in the safety evaluation of the HTGR hydrogen production systems were investigated and basic principles to establish acceptance criteria for the explosion and toxic gas release accidents were provided. Especially for the explosion accident, quantitative criteria to the reactor building are proposed with relating sample calculation results. It is necessary to treat abnormal events occurred in the hydrogen production system as an 'external events to the nuclear plant' in order to classify the hydrogen production system as no-nuclear facility' and basic policy to meet such requirement was also provided. (author)

  4. A CAL Program to Teach the Basic Principles of Genetic Engineering--A Change from the Traditional Approach.

    Science.gov (United States)

    Dewhurst, D. G.; And Others

    1989-01-01

    An interactive computer-assisted learning program written for the BBC microcomputer to teach the basic principles of genetic engineering is described. Discussed are the hardware requirements software, use of the program, and assessment. (Author/CW)

  5. Effects of an additional small group discussion to cognitive achievement and retention in basic principles of bioethics teaching methods

    Directory of Open Access Journals (Sweden)

    Dedi Afandi

    2009-03-01

    Full Text Available Aim The place of ethics in undergraduate medical curricula is essential but the methods of teaching medical ethics did not show substantial changes. “Basic principles of bioethics” is the best knowledge to develop student’s reasoning analysis in medical ethics In this study, we investigate the effects of an additional small group discussion in basic principles of bioethics conventional lecture methods to cognitive achievement and retention. This study was a randomized controlled trial with parallel design. Cognitive scores of the basic principles of bioethics as a parameter was measured using basic principles of bioethics (Kaidah Dasar Bioetika, KDB test. Both groups were attending conventional lectures, then the intervention group got an additional small group discussion.Result Conventional lectures with or without small group discussion significantly increased cognitive achievement of basic principles of bioethics (P= 0.001 and P= 0.000, respectively, and there were significant differences in cognitive achievement and retention between the 2 groups (P= 0.000 and P= 0.000, respectively.Conclusion Additional small group discussion method improved cognitive achievement and retention of basic principles of bioethics. (Med J Indones 2009; 18: 48-52Keywords: lecture, specification checklist, multiple choice questions

  6. Basic Principle of Advanced Oxidation Technology : Hybrid Technology Based on Ozone and Titania

    International Nuclear Information System (INIS)

    Widdi Usada; Agus Purwadi

    2007-01-01

    One of problems in health environment is organic liquid waste from many pollutant resources. Environmental friendly technology for degrading this waste is ozone which produced by plasma discharge technology, but its capability is limited. However, it is needed a new environmental friendly technology which has stronger capability. This new technology is so called advanced oxidation technology. Advanced oxidation technology is a hybrid of ozone, peroxide, UV light and photo catalyst. In this paper, it is introduced basic principle of hybrid of ozone and titania photo catalyst semiconductor. The capability of organic liquid degradation will be stronger because there is new radical which is produced by chemical reaction between electron-hole pair from photo catalyst titania and water or oxygen. This new radical then degrades this organic pollutant. This technology is used to degrade phenol. (author)

  7. Basic Principles of Electrical Network Reliability Optimization in Liberalised Electricity Market

    Science.gov (United States)

    Oleinikova, I.; Krishans, Z.; Mutule, A.

    2008-01-01

    The authors propose to select long-term solutions to the reliability problems of electrical networks in the stage of development planning. The guide lines or basic principles of such optimization are: 1) its dynamical nature; 2) development sustainability; 3) integrated solution of the problems of network development and electricity supply reliability; 4) consideration of information uncertainty; 5) concurrent consideration of the network and generation development problems; 6) application of specialized information technologies; 7) definition of requirements for independent electricity producers. In the article, the major aspects of liberalized electricity market, its functions and tasks are reviewed, with emphasis placed on the optimization of electrical network development as a significant component of sustainable management of power systems.

  8. Basic principles of thermo-acoustic energy and temporal profile detection of microwave pulses

    CERN Document Server

    Andreev, V G; Vdovin, V A

    2001-01-01

    Basic principles of a thermo-acoustic method developed for the detection of powerful microwave pulses of nanosecond duration are discussed.A proposed method is based on the registration of acoustic pulse profile originated from the thermal expansion of the volume where microwave energy was absorbed.The amplitude of excited acoustic transient is proportional to absorbed microwave energy and its temporal profile resembles one of a microwave pulse when certain conditions are satisfied.The optimal regimes of microwave pulse energy detection and sensitivity of acoustic transient registration with piezo-transducer are discussed.It was demonstrated that profile of a microwave pulse could be detected with temporal resolution of 1 - 3 nanosecond.

  9. The preanalytic phase in veterinary clinical pathology.

    Science.gov (United States)

    Braun, Jean-Pierre; Bourgès-Abella, Nathalie; Geffré, Anne; Concordet, Didier; Trumel, Cathy

    2015-03-01

    This article presents the general causes of preanalytic variability with a few examples showing specialists and practitioners that special and improved care should be given to this too often neglected phase. The preanalytic phase of clinical pathology includes all the steps from specimen collection to analysis. It is the phase where most laboratory errors occur in human, and probably also in veterinary clinical pathology. Numerous causes may affect the validity of the results, including technical factors, such as the choice of anticoagulant, the blood vessel sampled, and the duration and conditions of specimen handling. While the latter factors can be defined, influence of biologic and physiologic factors such as feeding and fasting, stress, and biologic and endocrine rhythms can often not be controlled. Nevertheless, as many factors as possible should at least be documented. The importance of the preanalytic phase is often not given the necessary attention, although the validity of the results and consequent clinical decision making and medical management of animal patients would likely be improved if the quality of specimens submitted to the laboratory was optimized. © 2014 American Society for Veterinary Clinical Pathology.

  10. Three-dimensional attached viscous flow basic principles and theoretical foundations

    CERN Document Server

    Hirschel, Ernst Heinrich; Kordulla, Wilhelm

    2014-01-01

    Viscous flow is usually treated in the frame of boundary-layer theory and as a two-dimensional flow. At best, books on boundary layers provide the describing equations for three-dimensional boundary layers, and solutions only for certain special cases.   This book presents the basic principles and theoretical foundations of three-dimensional attached viscous flows as they apply to aircraft of all kinds. Though the primary flight speed range is that of civil air transport vehicles, flows past other flying vehicles up to hypersonic speeds are also considered. Emphasis is put on general three-dimensional attached viscous flows and not on three-dimensional boundary layers, as this wider scope is necessary in view of the theoretical and practical problems that have to be overcome in practice.   The specific topics covered include weak, strong, and global interaction; the locality principle; properties of three-dimensional viscous flows; thermal surface effects; characteristic properties; wall compatibility con...

  11. Basic principles and criteria for public health protection in the event of a nuclear accident

    International Nuclear Information System (INIS)

    Kiradzhiev, G.

    1992-01-01

    Decision making criteria for population protection in nuclear accidents are discussed, and in particular the three basic principles: 1) excluding the appearance of nonstostochastic effects that occur in the case of high individual doses; 2) weighing the risks of radiation damage if such measures are not taken; 3) optimization based on comparison of benefit and costs, using the same measures for costs of health injury to affected populations and of the protected measures to be taken. The decision making criteria developed in Bulgaria are based on international recommendations with lowered upper limit of the range for evacuation and specified doses for vulnerable groups, children and pregnant women. The organization and the specific problems of the following individual types of protective measures are described: sheltering; protection of respiratory organs; iodine prophylaxis; evacuation of the public. One major condition for ensuring protection is to provide the public with timely information on the actual situation and the necessary countermeasures. Such information should be released in a manner that allows for understanding the expediency and significance of actions to be taken. An important aspect of emergency planning consists in taking into consideration the conditions actually prevailing in the country. This is well illustrated in the principle designated as 'national level of challenge' taking into account a country's capabilities for introducing intervention levels and permissible dose levels. In the case of Bulgaria this still remains to be done in protective planning for accidents. (author)

  12. THE BASIC PRINCIPLES OF THE INTEGRATED MANAGEMENT OF THE PROCESS OF ASSEMBLY AND THREADING

    Directory of Open Access Journals (Sweden)

    Anton Skorkin

    2017-11-01

    Full Text Available The subject matter of this article is the issues related to the integrated management of assembling operations of fastening and threading elements at all stages of their implementation. The goal is to develop the generalized structure of the data management system of the process of assembly and threading. The objectives are: to justify the principles of managing the assembly and threading process at each stage of the assembly to improve the efficiency of these operations, to study the power, accuracy and performance characteristics of the connections and to draw the conclusion that suggested the theory of assembly management is efficient. The following results are obtained. The article presents the analytical dependencies of the force indexes of threading in the course of the package and sheet assembly, including the tightening force while joining; the assembly of a multilayered package of dissimilar sheet materials was analyzed. On the basis of the theoretical analysis, the dependences of the power indices of threading during the package and sheet assembly were determined. The assembly of the package of sheet materials was investigated, including a multilayered package of dissimilar materials of a “metal-plastic” type. Conclusions. The process of assembling threaded joints with the use of management principles was used; these principles enable increasing the efficiency of the assembly process, reducing the complexity of the basic operations, and improving the quality of the joints obtained. The use adaptive control of the screwing speed on the main threading transitions is suggested for reducing the torque.  The technology of making threaded joints with given properties is developed, the main ways of increasing the efficiency of assembly and threading processes are determined on the basis of the integrated control system for the assembly process.

  13. A Symbiosis of Islamic Principles and Basic Human Values on Work and Life

    Directory of Open Access Journals (Sweden)

    Rokis Rohaiza

    2014-07-01

    Full Text Available The article discusses some basic issues on work and life based on Islamic and non-religious principles. Work and life are inseparable facets of and for human existence. Work derives from the demands of life, and thus it becomes a responsibility for every individual person. The article attempts to relate some highlighted Qur’anic verses and Hadith with basic occupational values such as honesty, modesty and innovativeness, among others, at workplaces. In the end, it makes an effort to understand the working of the subconscious mind on work to make life worthwhile. The plan for this article is to lay emphasis that the presence of human beings, which includes their mind, energy and whatever they can offer in the forms of work, benefits all. In such a case, not only those humans may be seen as successful in fulfilling the work-needs, but also victorious in completing the life-demands as human beings. “Virtuous workplace” is the most ideal platform for such purpose.

  14. 42 CFR 493.1249 - Standard: Preanalytic systems quality assessment.

    Science.gov (United States)

    2010-10-01

    ... 42 Public Health 5 2010-10-01 2010-10-01 false Standard: Preanalytic systems quality assessment. 493.1249 Section 493.1249 Public Health CENTERS FOR MEDICARE & MEDICAID SERVICES, DEPARTMENT OF HEALTH....1241 through 493.1242. (b) The preanalytic systems quality assessment must include a review of the...

  15. 42 CFR 493.1240 - Condition: Preanalytic systems.

    Science.gov (United States)

    2010-10-01

    ... 42 Public Health 5 2010-10-01 2010-10-01 false Condition: Preanalytic systems. 493.1240 Section 493.1240 Public Health CENTERS FOR MEDICARE & MEDICAID SERVICES, DEPARTMENT OF HEALTH AND HUMAN... of the preanalytic systems and correct identified problems as specified in § 493.1249 for each...

  16. Acceleration of cardiovascular MRI using parallel imaging: basic principles, practical considerations, clinical applications and future directions

    International Nuclear Information System (INIS)

    Niendorf, T.; Sodickson, D.

    2006-01-01

    Cardiovascular Magnetic Resonance (CVMR) imaging has proven to be of clinical value for non-invasive diagnostic imaging of cardiovascular diseases. CVMR requires rapid imaging; however, the speed of conventional MRI is fundamentally limited due to its sequential approach to image acquisition, in which data points are collected one after the other in the presence of sequentially-applied magnetic field gradients and radiofrequency coils to acquire multiple data points simultaneously, and thereby to increase imaging speed and efficiency beyond the limits of purely gradient-based approaches. The resulting improvements in imaging speed can be used in various ways, including shortening long examinations, improving spatial resolution and anatomic coverage, improving temporal resolution, enhancing image quality, overcoming physiological constraints, detecting and correcting for physiologic motion, and streamlining work flow. Examples of these strategies will be provided in this review, after some of the fundamentals of parallel imaging methods now in use for cardiovascular MRI are outlined. The emphasis will rest upon basic principles and clinical state-of-the art cardiovascular MRI applications. In addition, practical aspects such as signal-to-noise ratio considerations, tailored parallel imaging protocols and potential artifacts will be discussed, and current trends and future directions will be explored. (orig.)

  17. Principle and Basic Characteristics of a Hybrid Variable-Magnetic-Force Motor

    Science.gov (United States)

    Sakai, Kazuto; Kuramochi, Satoru

    Reduction in the power consumed by motors is important for energy saving in the case of electrical appliances and electric vehicles (EVs). The motors used for operating these devices operate at variable speeds. Further, the motors operate with a small load in the stationary mode and a large load in the starting mode. A permanent magnet motor can be operated at the rated power with a high efficiency. However, the efficiency is low at a small load or at a high speed because the large constant magnetic force results in substantial core loss. Furthermore, the flux-weakening current that decreases the voltage at a high speed leads to significant copper loss and core loss. Therefore, we have developed a new technique for controlling the magnetic force of a permanent magnet on the basis of the load or speed of the motor. In this paper, we propose a novel motor that can vary the magnetic flux of a permanent magnet and clarify the principle and basic characteristics of the motor. The new motor has a permanent magnet that is magnetized by the magnetizing coil of the stator. The analysis results show that the magnetic flux linkage of the motor can be changed from 37% to 100% that a high torque can be produced.

  18. EFLM WG-Preanalytical phase opinion paper

    DEFF Research Database (Denmark)

    Lippi, Giuseppe; Cornes, Michael P; Grankvist, Kjell

    2016-01-01

    The selection or procurement of blood collection devices in healthcare facilities is often an underestimated issue. This is probably due to different factors including the lack of knowledge of policymakers, hospital administrators and even laboratory managers about the importance of preanalytical...... quality and phlebotomy process, as well as to the absence of reliable guidelines or recommendations on how to precisely assess the quality of blood collection devices around the globe. With the awareness that a gap remains between manufacturers' and local validation of blood collection devices...

  19. A Study to Determine the Current Level of Implementation of Eighteen Basic Middle School Principles in the State of Missouri.

    Science.gov (United States)

    Beckman, Vernal G.

    The current level of implementation of 18 basic middle school principles in the 147 Missouri schools that met the definition of middle schools is the focus of this study. Questionnaire responses were received from 101 of the schools' administrators. Mean scores, standard deviations, and mean percentages of the maximum possible scores yielded by…

  20. Using the Chemistry of Fireworks to Engage Students in Learning Basic Chemical Principles: A Lesson in Eco-Friendly Pyrotechnics

    Science.gov (United States)

    Steinhauser, Georg; Klapotke, Thomas M.

    2010-01-01

    Fascination with fireworks and pyrotechnics can be used for educational purposes. Several aspects of pyrochemistry such as redox reactions, flame colors, or the theory of combustion can be incorporated in the curriculum to illustrate some basic chemical principles, guaranteeing a lesson that will be engaging and memorable. Beyond classic…

  1. Basic regulatory principles of Escherichia coli's electron transport chain for varying oxygen conditions.

    Science.gov (United States)

    Henkel, Sebastian G; Ter Beek, Alexander; Steinsiek, Sonja; Stagge, Stefan; Bettenbrock, Katja; de Mattos, M Joost Teixeira; Sauter, Thomas; Sawodny, Oliver; Ederer, Michael

    2014-01-01

    For adaptation between anaerobic, micro-aerobic and aerobic conditions Escherichia coli's metabolism and in particular its electron transport chain (ETC) is highly regulated. Although it is known that the global transcriptional regulators FNR and ArcA are involved in oxygen response it is unclear how they interplay in the regulation of ETC enzymes under micro-aerobic chemostat conditions. Also, there are diverse results which and how quinones (oxidised/reduced, ubiquinone/other quinones) are controlling the ArcBA two-component system. In the following a mathematical model of the E. coli ETC linked to basic modules for substrate uptake, fermentation product excretion and biomass formation is introduced. The kinetic modelling focusses on regulatory principles of the ETC for varying oxygen conditions in glucose-limited continuous cultures. The model is based on the balance of electron donation (glucose) and acceptance (oxygen or other acceptors). Also, it is able to account for different chemostat conditions due to changed substrate concentrations and dilution rates. The parameter identification process is divided into an estimation and a validation step based on previously published and new experimental data. The model shows that experimentally observed, qualitatively different behaviour of the ubiquinone redox state and the ArcA activity profile in the micro-aerobic range for different experimental conditions can emerge from a single network structure. The network structure features a strong feed-forward effect from the FNR regulatory system to the ArcBA regulatory system via a common control of the dehydrogenases of the ETC. The model supports the hypothesis that ubiquinone but not ubiquinol plays a key role in determining the activity of ArcBA in a glucose-limited chemostat at micro-aerobic conditions.

  2. Balancing knowledge and basic principles in veterinary parasitology - Competencies for future Danish veterinary graduates.

    Science.gov (United States)

    Thamsborg, Stig Milan; Johansen, Maria Vang; Nejsum, Peter; Williams, Andrew R; Mejer, Helena

    2018-03-15

    Veterinary parasitology has always been considered to be relevant and interesting by the Danish veterinary students. Students have to acquaint themselves with many new, small creatures with complicated and varied life cycles and with intricate Latin names that are difficult to pronounce, as only a few parasites have Danish names. In our veterinary curriculum, zoology has disappeared as a discipline, and parasitology has gradually moved from the third year to the beginning of the second year, which implies that, for example, pathology and pharmacology are "unknown fields". The number of contact hours in veterinary parasitology has been gradually cut to 24 lectures (35 min each) and practical exercises (24 h), including 9 h on coprology. The course is taught and examined jointly with bacteriology and virology in a 8-week course. As a comprehensive course, it has become increasingly difficult to get students to acquire enough active knowledge of the most common parasites and an understanding of the basic principles in relation to, for example, transmission and control. Even though information is readily accessible through books and on-line resources, we still believe that a competent clinician should know a range of parasites by heart as an active resource for their work. The dilemma has been tackled (partly) by introducing a veterinary paraclinical refresher course of 18 h (half practicals and half lectures) in the fourth study year. The focus here is on host(herd)-oriented clinical and diagnostic parasitology. The students can also now select a One Health track for six months in which zoonotic parasites are obviously a relevant topic. Copyright © 2018. Published by Elsevier B.V.

  3. Some aspects of metallurgical assessment of boiler tubes-Basic principles and case studies

    International Nuclear Information System (INIS)

    Chaudhuri, Satyabrata

    2006-01-01

    Microstructural changes in boiler tubes during prolong operation at high temperature and pressure decrease load bearing capacity limiting their useful lives. When the load bearing capacity falls below a critical level depending on operating parameters and tube geometry, failure occurs. In order to avoid such failures mainly from the view point of economy and safety, this paper describes some basic principles behind remaining life assessment of service exposed components and also a few case studies related to failure of a reheater tube of 1.25Cr-0.5Mo steel, a carbon steel tube and final superheater tubes of 2.25Cr-1Mo steel and remaining creep life assessment of service exposed but unfailed platen superheater and reheater tubes of 2.25Cr-1Mo steel. Sticking of fly ash particles causing reduction in effective tube wall thickness is responsible for failure of reheater tubes. Decarburised metal containing intergranular cracks at the inner surface of the carbon steel tube exhibiting a brittle window fracture is an indicative of hydrogen embrittlement responsible for this failure. In contrast, final superheater tube showed that the failure took place due to short-term overheating. The influence of prolong service revealed that unfailed reheater tubes exhibit higher tensile properties than that of platen superheater tubes. In contrast both the tubes at 50 MPa meet the minimum creep rupture properties when compared with NRIM data. The remaining creep life of platen superheater tube as estimated at 50 MPa and 570 deg. C (1058 o F) is more than 10 years and that of reheater tube at 50 MPa and 580 deg. C (1076 o F) is 9 years

  4. The presence of communication in the basic organizational principles of the University of Zulia towards the student sector

    Directory of Open Access Journals (Sweden)

    Laura Castellano Ramírez

    2006-11-01

    Full Text Available This article is centered in detecting the presence of comunication towards the student sector, like a formal element in the redaccion of the organization basic principles (OBP of La Universidad del Zulia, such as Mision, Vision, Values, Polítics, Objectives and Strategies. Starting from the theoric approach of that the OBP conform the basic component of the Corporative Identity, in wish are rised the other three: the organizational beheivier, the simbology or visual identity and the comunication. Through the documentary research it has concluded that the communication towards the student community is not taken in count in the redaction of the basic principles, it is mentioned only in the comunicational politic but in a very general way.

  5. Preanalytical Variables Affecting the Integrity of Human Biospecimens in Biobanking

    DEFF Research Database (Denmark)

    Ellervik, Christina; Vaught, Jim

    2015-01-01

    BACKGROUND: Most errors in a clinical chemistry laboratory are due to preanalytical errors. Preanalytical variability of biospecimens can have significant effects on downstream analyses, and controlling such variables is therefore fundamental for the future use of biospecimens in personalized...... medicine for diagnostic or prognostic purposes. CONTENT: The focus of this review is to examine the preanalytical variables that affect human biospecimen integrity in biobanking, with a special focus on blood, saliva, and urine. Cost efficiency is discussed in relation to these issues. SUMMARY: The quality...

  6. Is a pre-analytical process for urinalysis required?

    Science.gov (United States)

    Petit, Morgane; Beaudeux, Jean-Louis; Majoux, Sandrine; Hennequin, Carole

    2017-10-01

    For the reliable urinary measurement of calcium, phosphate and uric acid, a pre-analytical process by adding acid or base to urine samples at laboratory is recommended in order to dissolve precipitated solutes. Several studies on different kind of samples and analysers have previously shown that a such pre-analytical treatment is useless. The objective was to study the necessity of pre-analytical treatment of urine on samples collected using the V-Monovette ® (Sarstedt) system and measured on the analyser Architect C16000 (Abbott Diagnostics). Sixty urinary samples of hospitalized patients were selected (n=30 for calcium and phosphate, and n=30 for uric acid). After acidification of urine samples for measurement of calcium and phosphate, and alkalinisation for measurement of uric acid respectively, differences between results before and after the pre-analytical treatment were compared to acceptable limits recommended by the French society of clinical biology (SFBC). No difference in concentration between before and after pre-analytical treatment of urine samples exceeded acceptable limits from SFBC for measurement of calcium and uric acid. For phosphate, only one sample exceeded these acceptable limits, showing a result paradoxically lower after acidification. In conclusion, in agreement with previous study, our results show that acidification or alkalinisation of urine samples from 24 h urines or from urination is not a pre-analytical necessity for measurement of calcium, phosphate and uric acid.

  7. PROJECTING PRINCIPLES OF «MATHEMATICAL AND PHYSICAL BASICS IN INFORMATICS» MODULE AS PART OF A UNIVERSAL BACCALAUREATE PROGRAM

    Directory of Open Access Journals (Sweden)

    E. K. Samerkhanova

    2016-01-01

    Full Text Available This article is a presentation of “Mathematical and Physical Basics in Informatics” Module projected as part of universal baccalaureate model realized in Minin State Pedagogical University, Nizhny Novgorod, within the educational program for an extended group of qualifications “Informatics and Calculating Machines”. Module authors defined a strategy for baccalaureates preparation in the field of informational technology, which is realized due to a bunch of principles: education fundamentalization, learning systematization, activity, integration and student-centration. The principle of education fundamentalization requires consolidation of scientific and methodological preparation of students. The principle of learning systematization guarantees formation of students’ holistic ideology. The principle of integration is directed onto qualitative interdisciplinary conversion based on mutual enrichment of knowledge and skills. The principle of activity considers student personality, its formation and growth with respect to practical activity as a special form of psychical activity. The principle of student-centration is displayed as social-humanistic purpose of the educational process.

  8. An introduction to the basic principles of health economics for those involved in the development and delivery of headache care.

    Science.gov (United States)

    Kernick, D

    2005-09-01

    Against a background of increasing demands on limited resources, health economics is gaining an increasing impact on decision making and a basic understanding of the subject is important for all those involved in headache research and service delivery at whatever level. This paper is not intended as a review of the literature in the area of headache economics but discusses some general principles of health economics from the perspective of headache, with a focus on cost of illness studies and economic evaluation.

  9. SPECIALIZED MAPPING OF CRUSTAL FAULT ZONES. PART 1: BASIC THEORETICAL CONCEPTS AND PRINCIPLES

    Directory of Open Access Journals (Sweden)

    K. Zh. Seminsky

    2014-01-01

    Full Text Available Long-term studies of shear zones have included collection of data on fractures showing no indication of displacement which are termed as 'blank' fractures. A method aimed at mapping fault structures and stress fields has been developed on the basis of results of paragenetic analysis of measurements of abundant fractures. The method is termed as 'specialized mapping', firstly, due to its specific structural goal so that to distinguish it from the conventional geological mapping of regions in nature, and, secondly, because of the specific procedure applied to refer to fractures as references to decipher fault-block patterns of natural regions. In Part 1, basic theoretical concepts and principles of specialized mapping are described. Part 2 is being prepared for publication in one of the next issues of the journal; it will cover stages of the proposed method and describe some of the cases of its application.In terms of general organizational principles, specialized mapping is similar to other methods based on structural paragenetic analysis and differs from such methods in types of paragenesises viewed as references to reveal crustal fault zones. Such paragenesises result from stage-by-stage faulting (Fig 2 and Fig. 7 during which stress fields of the 2nd order are regularly changeable within the shear zone. According to combined experimental and natural data, a complete paragenesis of fractures in the shear zone includes a major (1st order fault plane and fractures of other seven types, R, R’, n, n’, t, t’ and T (2nd order (Fig. 4 and Fig 8. At the fracture level, each of them corresponds to a paragenesis including three nearly perpendicular systems of early ruptures (Fig. 1, which are based on two classical patterns of conjugated fractures, one of which is consistent with the position of the fault plane (Fig. 3. Taking into account that strike-slip, reverse and normal faults are similar in terms of mechanics (i.e. they are formed due to

  10. Basic Principles of Financial Planning in Ex-ante Deposit Insurance Schemes

    Directory of Open Access Journals (Sweden)

    Đurđica Ognjenović

    2006-12-01

    Full Text Available The paper explores main principles of financial planning in ex-ante deposit insurance schemes from a theoretical perspective and in terms of the EU Directive on deposit- guarantee schemes. Further on, the paper assesses how these principles and standards are used in financial planning in deposit insurance schemes around the world for annual budgeting, strategic planning and optimalization of available financial resources. After reviewing available references and different practices, the conclusion is that there are no clear internationally accepted principles for deposit insurers’ financial planning, except some broad and general guidelines. Practices in the industry differ significantly. Given the fact that deposit insurance is in fact a monopolistic business, lack of clear principles and lack of proper financial planning may lead to inadequacy of ex-ante funds and negligence on the side of the management of deposit insurance schemes.

  11. Radiolabeling Silica-Based Nanoparticles via Coordination Chemistry: Basic Principles, Strategies, and Applications.

    Science.gov (United States)

    Ni, Dalong; Jiang, Dawei; Ehlerding, Emily B; Huang, Peng; Cai, Weibo

    2018-03-20

    As one of the most biocompatible and well-tolerated inorganic nanomaterials, silica-based nanoparticles (SiNPs) have received extensive attention over the last several decades. Recently, positron emission tomography (PET) imaging of radiolabeled SiNPs has provided a highly sensitive, noninvasive, and quantitative readout of the organ/tissue distribution, pharmacokinetics, and tumor targeting efficiency in vivo, which can greatly expedite the clinical translation of these promising NPs. Encouraged by the successful PET imaging of patients with metastatic melanoma using 124 I-labeled ultrasmall SiNPs (known as Cornell dots or C dots) and their approval as an Investigational New Drug (IND) by the United States Food and Drug Administration, different radioisotopes ( 64 Cu, 89 Zr, 18 F, 68 Ga, 124 I, etc.) have been reported to radiolabel a wide variety of SiNPs-based nanostructures, including dense silica (dSiO 2 ), mesoporous silica (MSN), biodegradable mesoporous silica (bMSN), and hollow mesoporous silica nanoparticles (HMSN). With in-depth knowledge of coordination chemistry, abundant silanol groups (-Si-O-) on the silica surface or inside mesoporous channels not only can be directly used for chelator-free radiolabeling but also can be readily modified with the right chelators for chelator-based labeling. However, integrating these labeling strategies for constructing stably radiolabeled SiNPs with high efficiency has proven difficult because of the complexity of the involved key parameters, such as the choice of radioisotopes and chelators, nanostructures, and radiolabeling strategy. In this Account, we present an overview of recent progress in the development of radiolabeled SiNPs for cancer theranostics in the hope of speeding up their biomedical applications and potential translation into the clinic. We first introduce the basic principles and mechanisms for radiolabeling SiNPs via coordination chemistry, including general rules of selecting proper

  12. BASIC

    DEFF Research Database (Denmark)

    Hansen, Pelle Guldborg; Schmidt, Karsten

    2017-01-01

    De sidste 10 år har vi været vidner til opkomsten af et nyt evidensbaseret policy paradigme, Behavioural Public Policy (BPP), der søger at integrere teoretiske og metodiske indsigter fra adfærdsvidenskaberne i offentlig politikudvikling. Arbejdet med BPP har dog båret præg af, at være usystematisk...... BPP. Tilgangen består dels af den overordnede proces-model BASIC og dels af et iboende framework, ABCD, der er en model for systematisk adfærdsanalyse, udvikling, test og implementering af adfærdsrettede løsningskoncepter. Den samlede model gør det muligt for forskere såvel som offentligt ansatte...

  13. The basic principle and criteria for ore prospecting of uranium neutralizing-reduction mineralization

    International Nuclear Information System (INIS)

    Shi Weijun; Zhou Wenbin; Yuan Xiaoqin

    1992-01-01

    The author discuss the geological criteria, alternated mineral and geochemical criteria of field recognition which is beneficial to the uranium neutralizing-reduction mineralization sectors on the basis of brief introduction to the principle of uranium neutralizing-reduction mineralization, and the geological significance of uranium neutralizing-reduction mineralization is also stated

  14. Basic principles of STT-MRAM cell operation in memory arrays

    International Nuclear Information System (INIS)

    Khvalkovskiy, A V; Apalkov, D; Watts, S; Chepulskii, R; Beach, R S; Ong, A; Tang, X; Driskill-Smith, A; Lottis, D; Chen, E; Nikitin, V; Krounbi, M; Butler, W H; Visscher, P B

    2013-01-01

    For reliable operation, individual cells of an STT-MRAM memory array must meet specific requirements on their performance. In this work we review some of these requirements and discuss the fundamental physical principles of STT-MRAM operation, covering the range from device level to chip array performance, and methodology for its development. (paper)

  15. Functional Assessment of Corticospinal Conduction with Transcranial Magnetic Stimulation: Basic Principles

    DEFF Research Database (Denmark)

    Groppa, S.; Peller, M.; Siebner, Hartwig R.

    2010-01-01

    Here we review how transcranial magnetic stimulation (TMS) is used in clinical practice to examine the functional integrity of the fast conducting fibres of the human corticomotor path ways. We first summarise the technical and physiological principles of TMS that are relevant to its clinical use...

  16. Effect of an intervention based on basic Buddhist principles on the spiritual well-being of patients with terminal cancer.

    Science.gov (United States)

    Chimluang, Janya; Thanasilp, Sureeporn; Akkayagorn, Lanchasak; Upasen, Ratchaneekorn; Pudtong, Noppamat; Tantitrakul, Wilailuck

    2017-12-01

    To evaluate the effect of an intervention based on basic Buddhist principles on the spiritual well-being of patients with terminal cancer. This quasi-experimental research study had pre- and post-test control groups. The experimental group received conventional care and an intervention based on basic Buddhist principles for three consecutive days, including seven activities based on precept activities, concentration activities and wisdom activities. The control group received conventional care alone. Forty-eight patients participated in this study: 23 in the experimental group and 25 in the control group. Their mean age was 53 (standard deviation 10) years. The spiritual well-being of participants in the experimental group was significantly higher than that of participants in the control group at the second post-test (P principles improved the spiritual well-being of patients with terminal cancer. This result supports the beneficial effects of implementing this type of intervention for patients with terminal cancer. Copyright © 2017 Elsevier Ltd. All rights reserved.

  17. The application of redundancy-related basic safety principles to the 1400 MWE reactor core standby cooling system

    International Nuclear Information System (INIS)

    Bertrand, R.

    1990-01-01

    This memorandum shall provide the background for the work of the European Community Commission which is to analyze safety principles relating to redundancy. The redundancy-related basic safety principles applied in French nuclear power plants are the following: . the single-failure criterion, . provisions additional to application of the single-failure criterion. These are mainly provisions made at the design stage to minimize risks associated with common cause failures or the risks of human error which can lead to such failures: - protection against hazards of internal and external origin, - the geographical or physical separation of equipment, - the independence of electrical power supplies and distribution systems, - the additional resources and associated operating procedures making it possible to accommodate total loss of the safety systems. The scope also includes the operating rules which ensure availability of redundant safety-related equipment. The provisions relating to the single-failure criterion are detailed in Basic Safety Rule 1.3.A appended. The application of these principles proposed by the operating organization and accepted by the safety authorities for the design and operation of the standby core cooling system (System RIS) is explained

  18. Identifying Students' Conceptions of Basic Principles in Sequence Stratigraphy

    Science.gov (United States)

    Herrera, Juan S.; Riggs, Eric M.

    2013-01-01

    Sequence stratigraphy is a major research subject in the geosciences academia and the oil industry. However, the geoscience education literature addressing students' understanding of the basic concepts of sequence stratigraphy is relatively thin, and the topic has not been well explored. We conducted an assessment of 27 students' conceptions of…

  19. Balancing equity and efficiency in the Dutch basic benefits package using the principle of proportional shortfall.

    Science.gov (United States)

    van de Wetering, E J; Stolk, E A; van Exel, N J A; Brouwer, W B F

    2013-02-01

    Economic evaluations are increasingly used to inform decisions regarding the allocation of scarce health care resources. To systematically incorporate societal preferences into these evaluations, quality-adjusted life year gains could be weighted according to some equity principle, the most suitable of which is a matter of frequent debate. While many countries still struggle with equity concerns for priority setting in health care, the Netherlands has reached a broad consensus to use the concept of proportional shortfall. Our study evaluates the concept and its support in the Dutch health care context. We discuss arguments in the Netherlands for using proportional shortfall and difficulties in transitioning from principle to practice. In doing so, we address universal issues leading to a systematic consideration of equity concerns for priority setting in health care. The article thus has relevance to all countries struggling with the formalization of equity concerns for priority setting.

  20. The Clinical Value, Principle, and Basic Practical Technique of Mindfulness Intervention.

    Science.gov (United States)

    Zou, Tao; Wu, Chenghan; Fan, Xiaoduo

    2016-06-25

    Mindfulness intervention is a psychotherapy based on the Buddhist practice of meditation, combining the theories and methodology of contemporary psychology. The empirical research in recent years has indicated that mindfulness intervention yields favorable results including reduction of depression relapse, alleviation of the symptoms of depression and anxiety, reduction of substance abuse, relief of pain, blood pressure management, enhancement of immunity, and improvement of sleep. Currently, mindfulness therapy has become the mainstream of psychotherapy in the realm of European and American psychotherapy. The fields of psychology and psychotherapy in China have also begun to introduce mindfulness intervention in recent years. However, there is a lack of relevant practice and research in the field of clinical mental health. This article will briefly introduce the concept of mindfulness, the basic mechanism of the intervention, and the basic skills and guidelines in clinical practice.

  1. Diffusion Tensor Imaging and Its Application to Traumatic Brain Injury: Basic Principles and Recent Advances

    Science.gov (United States)

    2012-12-01

    an advanced magnetic resonance imaging (MRI) technique that is sensitive to the movement of water molecules, providing additional information on the...imaging (MRI) technique that is sensitive to the movement of water molecules, providing additional information on the micro-structural arrangement of...disrupted fiber tracts adjacent to the hemorrhages. 1.3. Basic Physics of DW-MRI and Apparent Diffusion Coefficient (ADC) Mapping Brownian motion of

  2. On psychological growth and vulnerability: basic psychological need satisfaction and need frustration as an unifying principle

    OpenAIRE

    Vansteenkiste, Maarten; Ryan, Richard

    2013-01-01

    Humans have a potential for growth, integration, and well-being, while also being vulnerable to defensiveness, aggression, and ill-being. Self-determination theory (R. M. Ryan & E. L. Deci, 2000, Self-determination theory and the facilitation of intrinsic motivation, social development and well-being, American Psychologist, Vol. 55, pp. 68–78) argues that satisfaction of the basic psychological needs for autonomy, competence, and relatedness both fosters immediate well-being and strengthens i...

  3. Basic strategical principles regarding Romanian power development after the year 2000

    International Nuclear Information System (INIS)

    Hotopeleanu, S.; Petrescu, C.

    1996-01-01

    This study deals with the the basic strategies for long term power planning in Romanian economy taking into account socio-economic factors, refurbishment and restructuring, having in view the requirements of free market economy transition. The main directions of this strategy refer to the development of the present power capacities based on fossil fuel burning and hydroelectric power and also to the development of nuclear power plants based on CANDU type reactors. (author) 3 tabs

  4. Use of basic principle of nucleation in determining temperature-threshold neutron energy relationship in superheated emulsions

    CERN Document Server

    Das, M; Chatterjee, B K; Roy, S C

    2003-01-01

    Detection of neutrons through use of superheated emulsions has been known for about two decades. The minimum neutron energy (threshold) required to nucleate drops of a given liquid has a dependence on the temperature of the liquid. The basic principle of nucleation has been utilized to find the relationship between the operating temperature and threshold neutron energy for superheated emulsions made of R-114 liquid. The threshold energy thus determined for different temperatures has been compared with accurate experimental results obtained using monoenergetic neutron sources. The agreement is found to be satisfactory and confirms the applicability of the present simple method to other liquids.

  5. Neutrons in studies of phospholipid bilayers and bilayer–drug interaction. I. Basic principles and neutron diffraction

    Directory of Open Access Journals (Sweden)

    Belička M.

    2014-12-01

    Full Text Available In our paper, we demonstrate several possibilities of using neutrons in pharmaceutical research with the help of examples of scientific results achieved at our University. In this first part, basic properties of neutrons and elementary principles of elastic scattering of thermal neutrons are described. Results of contrast variation neutron diffraction on oriented phospholipid bilayers with intercalated local anaesthetic or cholesterol demonstrate the potential of this method at determination of their position in bilayers. Diffraction experiments with alkan-1-ols located in the bilayers revealed their influence on bilayer thickness as a function of their alkyl chain length.

  6. High-energy cosmic rays and tests of basic principles of Physics

    Directory of Open Access Journals (Sweden)

    Gonzalez-Mestres L.

    2014-04-01

    Full Text Available With the present understanding of data, the observed flux suppression for ultra-high energy cosmic rays (UHECR at energies above 4.1019 eV can be a signature of the Greisen-Zatsepin-Kuzmin (GZK cutoff or be related to a similar mechanism. But it may also correspond, for instance, to the maximum energies available at the relevant sources. In both cases, violations of special relativity modifying cosmic-ray propagation or acceleration at very high energy can potentially play a role. Other violations of fundamental principles of standard particle physics (quantum mechanics, energy and momentum conservation, vacuum homogeneity and “static” properties, effective space dimensions, quark confinement… can also be relevant at these energies. In particular, UHECR data would in principle allow to set bounds on Lorentz symmetry violation (LSV in patterns incorporating a privileged local reference frame (the “vacuum rest frame”, VRF. But the precise analysis is far from trivial, and other effects can also be present. The effective parameters can be related to Planckscale physics, or even to physics beyond Planck scale, as well as to the dynamics and effective symmetries of LSV for nucleons, quarks, leptons and the photon. LSV can also be at the origin of GZK-like effects. In the presence of a VRF, and contrary to a “grand unification” view, LSV and other violations of standard principles can modify the internal structure of particles at very high energy and conventional symmetries may cease to be valid at energies close to the Planck scale. We present an updated discussion of these topics, including experimental prospects, new potentialities for high-energy cosmic ray phenomenology and the possible link with unconventional pre-Big Bang scenarios, superbradyon (superluminal preon patterns… The subject of a possible superluminal propagation of neutrinos at accelerator energies is also dealt with.

  7. Basic principles of magnetic resonance imaging in cerebral ischemia and initial clinical experience

    International Nuclear Information System (INIS)

    Brant-Zawadzki, M.; Solomon, M.; Newton, T.H.; Weinstein, P.; Schmidley, J.; Norman, D.

    1985-01-01

    NMR tomography proved to be a method superior to computerized tomography (CT) in terms of sensitivity in the diagnosis of pathological changes in the brain. The article on hand gives a brief survey on the principle of the method and demonstrates the superiority of NMR tomography to CT exemplified by images taken from a number of cases. Instrumental factors influencing diagnosis of cerebral ischaemie are discussed. Clinical studies performed on more than 20 partients with suspected infarct revealed the superiority of NMR tomography to CT. (MG) [de

  8. The Emerging Role of Tractography in Deep Brain Stimulation: Basic Principles and Current Applications

    Directory of Open Access Journals (Sweden)

    Nelson B. Rodrigues

    2018-01-01

    Full Text Available Diffusion tensor imaging (DTI is an MRI-based technique that delineates white matter tracts in the brain by tracking the diffusion of water in neural tissue. This methodology, known as “tractography”, has been extensively applied in clinical neuroscience to explore nervous system architecture and diseases. More recently, tractography has been used to assist with neurosurgical targeting in functional neurosurgery. This review provides an overview of DTI principles, and discusses current applications of tractography for improving and helping develop novel deep brain stimulation (DBS targets.

  9. Application of basic physics principles to clinical neuroradiology: differentiating artifacts from true pathology on MRI.

    Science.gov (United States)

    Hakky, Michael; Pandey, Shilpa; Kwak, Ellie; Jara, Hernan; Erbay, Sami H

    2013-08-01

    This article outlines artifactual findings commonly encountered in neuroradiologic MRI studies and offers clues to differentiate them from true pathology on the basis of their physical properties. Basic MR physics concepts are used to shed light on the causes of these artifacts. MRI is one of the most commonly used techniques in neuroradiology. Unfortunately, MRI is prone to image distortion and artifacts that can be difficult to identify. Using the provided case illustrations, practical clues, and relevant physical applications, radiologists may devise algorithms to troubleshoot these artifacts.

  10. Basic mapping principles for visualizing cancer data using Geographic Information Systems (GIS).

    Science.gov (United States)

    Brewer, Cynthia A

    2006-02-01

    Maps and other data graphics may play a role in generating ideas and hypotheses at the beginning of a project. They are useful as part of analyses for evaluating model results and then at the end of a project when researchers present their results and conclusions to varied audiences, such as their local research group, decision makers, or a concerned public. Cancer researchers are gaining skill with geographic information system (GIS) mapping as one of their many tools and are broadening the symbolization approaches they use for investigating and illustrating their data. A single map is one of many possible representations of the data, so making multiple maps is often part of a complete mapping effort. Symbol types, color choices, and data classing each affect the information revealed by a map and are best tailored to the specific characteristics of data. Related data can be examined in series with coordinated classing and can also be compared using multivariate symbols that build on the basic rules of symbol design. Informative legend wording and setting suitable map projections are also basic to skilled mapmaking.

  11. THE CHALLENGES OF BUSINESS ETHICS: THE BASIC PRINCIPLES OF BUSINESS ETHICS - ETHICAL CODEX IN BUSINESS

    Directory of Open Access Journals (Sweden)

    Anton Jamnik

    2017-09-01

    Full Text Available Past several decades management has become a vital concern to society. If we look at pools, we notice that the public does not have good opinion about management ethics and business. For the management community to turn this situation around, significant efforts are required. It should be understood what management ethics means, why it is important and how it should be integrated into decision making. Principles of ethics from moral philosophy and management theory are available to inform interested managers. Further requires that managers understand, and be sensitive to, all the stakeholders of the organization and their stakes. If the moral management model is to be achieved, managers need to integrate ethical wisdom with their managerial wisdom and to take steps to create and sustain an ethical climate in their organizations. When all that will be done, the desirable goals of moral management will be achievable.

  12. Principle and Basic Characteristics of Variable-Magnetic-Force Memory Motors

    Science.gov (United States)

    Sakai, Kazuto; Yuki, Kazuaki; Hashiba, Yutaka; Takahashi, Norio; Yasui, Kazuya; Kovudhikulrungsri, Lilit

    A reduction in the power consumed by motors is required for energy saving in the case of electrical appliances and electric vehicles (EV). The motors used for operating these apparatus operate at variable speeds. Further, the motors operate with small load in stationary mode and with large load in start-up mode. A permanent magnet motor can operate at the rated power with a high efficiency. However, the efficiency is lower at small load or high speed because the large constant magnetic force results in substantial core loss. Furthermore, the flux-weakening current that depresses voltage at high speed leads to significant copper loss. Therefore, we have developed a new technique for controlling the magnetic force of permanent magnet on the basis of the load or speed of the motor. In this paper, we propose the novel motor that can vary magnetic flux and we clarify the principle.

  13. Basic safety principles of INSAG and their application in radioactive waste management

    International Nuclear Information System (INIS)

    Baer, A.J.

    2000-01-01

    The International Nuclear Safety Advisory Group (INSAG) has, in INSAG-11, attempted to show what safety principles are common to all applications of all sources of radiation. It has been considered that these general principles should apply to all industrial activities. A comparison of INSAG-11 with Article 11 of the Joint Convention on the Safety of Spent Fuel Management and on the Safety of Radioactive Waste Management (Joint Convention) shows that the management of radioactive waste is but a special case of industrial activity and follows the same safety rules. The importance of the Joint Convention comes, however, from the fact that it is a politically important document, requiring ratification by the parliaments of the contracting parties. The safe management of radioactive waste implies that five types of issue must be taken into consideration, not only technical and ethical ones, but also socio-political, economic and ecological ones. By comparison, sustainable development in its three dimensions (temporal, spatial and sectorial) has five components (ecology, economics, ethics, socio-politics and technology), just like the safe management of radioactive waste. The consequence of this is that if management is treated as a particular case of sustainable development, it will not be accepted by society. The conclusions are that technology alone can not ensure the safety of radioactive waste management and that society will always give priority to socio-political issues over technological ones. Furthermore, it is crucial that people involved in the management of radioactive waste learn to communicate better and to listen more attentively. Their efforts will only succeed when they incorporate all the components that determine the fabric of our society. (author)

  14. NATO Advanced Study Institute on Nano-Optics : Principles Enabling Basic Research and Applications

    CERN Document Server

    Collins, John; Silvestri, Luciano

    2017-01-01

    This book provides a comprehensive overview of nano-optics, including basic theory, experiment and applications, particularly in nanofabrication and optical characterization. The contributions clearly demonstrate how advances in nano-optics and photonics have stimulated progress in nanoscience and -fabrication, and vice versa. Their expert authors address topics such as three-dimensional optical lithography and microscopy beyond the Abbe diffraction limit, optical diagnostics and sensing, optical data- and telecommunications, energy-efficient lighting, and efficient solar energy conversion. Nano-optics emerges as a key enabling technology of the 21st century. This work will appeal to a wide readership, from physics through chemistry, to biology and engineering. The contributions that appear in this volume were presented at a NATO Advanced Study Institute held in Erice, 4-19 July, 2015.

  15. Advanced Energy Storage Devices: Basic Principles, Analytical Methods, and Rational Materials Design

    Science.gov (United States)

    Liu, Jilei; Wang, Jin; Xu, Chaohe; Li, Chunzhong; Lin, Jianyi

    2017-01-01

    Abstract Tremendous efforts have been dedicated into the development of high‐performance energy storage devices with nanoscale design and hybrid approaches. The boundary between the electrochemical capacitors and batteries becomes less distinctive. The same material may display capacitive or battery‐like behavior depending on the electrode design and the charge storage guest ions. Therefore, the underlying mechanisms and the electrochemical processes occurring upon charge storage may be confusing for researchers who are new to the field as well as some of the chemists and material scientists already in the field. This review provides fundamentals of the similarities and differences between electrochemical capacitors and batteries from kinetic and material point of view. Basic techniques and analysis methods to distinguish the capacitive and battery‐like behavior are discussed. Furthermore, guidelines for material selection, the state‐of‐the‐art materials, and the electrode design rules to advanced electrode are proposed. PMID:29375964

  16. Fuel cell technology for classroom instruction. Basic principles, experiments, work sheets. 2. ed.

    Energy Technology Data Exchange (ETDEWEB)

    Voigt, Cornelia; Hoeller, Stefan; Kueter, Uwe

    2009-07-01

    This book provides a clear introduction and overview to fuel cell technology and its associated subject areas. Examples of experiments using solar cells, electrolysis and fuel cells convey the knowledge for forthcoming tests in an understandable manner. The preparation of classroom experiments is made considerably easier for the teacher thanks to the experiment work sheets. These contain the necessary information concerning the material, set-up and execution of the experiment, and questions for evaluation purposes. Online-Shop The training documents and student work sheets combine the basic knowledge, questions and answers, and are ideal for copying. A comprehensive glossary at the end of the book explains all the important technical terms. (orig.)

  17. Chemistry Cube Game - Exploring Basic Principles of Chemistry by Turning Cubes.

    Science.gov (United States)

    Müller, Markus T

    2018-02-01

    The Chemistry Cube Game invites students at secondary school level 1 and 2 to explore basic concepts of chemistry in a playful way, either as individuals or in teams. It consists of 15 different cubes, 9 cubes for different acids, their corresponding bases and precursors, and 6 cubes for different reducing and oxidising agents. The cubes can be rotated in those directions indicated. Each 'allowed' vertical or horizontal rotation of 90° stands for a chemical reaction or a physical transition. Two different games and playing modes are presented here: First, redox chemistry is introduced for the formation of salts from elementary metals and non-metals. Second, the speciation of acids and bases at different pH-values is shown. The cubes can be also used for games about environmental chemistry such as the carbon and sulphur cycle, covering the topic of acid rain, or the nitrogen cycle including ammoniac synthesis, nitrification and de-nitrification.

  18. The basic principles of migration health: Population mobility and gaps in disease prevalence

    Directory of Open Access Journals (Sweden)

    MacPherson Douglas W

    2006-05-01

    Full Text Available Abstract Currently, migrants and other mobile individuals, such as migrant workers and asylum seekers, are an expanding global population of growing social, demographic and political importance. Disparities often exist between a migrant population's place of origin and its destination, particularly with relation to health determinants. The effects of those disparities can be observed at both individual and population levels. Migration across health and disease disparities influences the epidemiology of certain diseases globally and in nations receiving migrants. While specific disease-based outcomes may vary between migrant group and location, general epidemiological principles may be applied to any situation where numbers of individuals move between differences in disease prevalence. Traditionally, migration health activities have been designed for national application and lack an integrated international perspective. Present and future health challenges related to migration may be more effectively addressed through collaborative global undertakings. This paper reviews the epidemiological relationships resulting from health disparities bridged by migration and describes the growing role of migration and population mobility in global disease epidemiology. The implications for national and international health policy and program planning are presented.

  19. The basic principles of migration health: Population mobility and gaps in disease prevalence

    Science.gov (United States)

    Gushulak, Brian D; MacPherson, Douglas W

    2006-01-01

    Currently, migrants and other mobile individuals, such as migrant workers and asylum seekers, are an expanding global population of growing social, demographic and political importance. Disparities often exist between a migrant population's place of origin and its destination, particularly with relation to health determinants. The effects of those disparities can be observed at both individual and population levels. Migration across health and disease disparities influences the epidemiology of certain diseases globally and in nations receiving migrants. While specific disease-based outcomes may vary between migrant group and location, general epidemiological principles may be applied to any situation where numbers of individuals move between differences in disease prevalence. Traditionally, migration health activities have been designed for national application and lack an integrated international perspective. Present and future health challenges related to migration may be more effectively addressed through collaborative global undertakings. This paper reviews the epidemiological relationships resulting from health disparities bridged by migration and describes the growing role of migration and population mobility in global disease epidemiology. The implications for national and international health policy and program planning are presented. PMID:16674820

  20. Using Video Games to Support Pre-Service Elementary Teachers Learning of Basic Physics Principles

    Science.gov (United States)

    Anderson, Janice; Barnett, Michael

    2011-08-01

    The purpose of this work is to share our findings in using video gaming technology to facilitate the understanding of basic electromagnetism with pre-service elementary teachers. To this end we explored the impact of using a game called Supercharged! on pre-service teachers' understanding of electromagnetic concepts compared to students who conducted a more traditional inquiry oriented investigation of the same concepts. This study was a part of a larger design experiment examining the pedagogical potential of Supercharged! the control group learned through a series of guided inquiry methods while the experimental group played Supercharged! during the laboratory sections of the science course. There was significant difference F(2,134) = 4.8, p video games can lead to positive learning outcomes, as demonstrated by the increase in test scores from pre- to post-assessment. Additionally, this study also suggests that a complementary approach, in which video games and hands-on activities are integrated, with each activity informing the other, could be a very powerful technique for supporting student scientific understanding. Further, our findings suggest that video game designers should embed meta-cognitive activities such as reflective opportunities into educational video games to provide scaffolds for students and to reinforce that they are engaged in an educational learning experience.

  1. Goal Commitments and the Content of Thoughts and Dreams: Basic Principles

    Directory of Open Access Journals (Sweden)

    Eric eKlinger

    2013-07-01

    Full Text Available A few empirically supported principles can account for much of the thematic content of waking thought, including rumination, and dreams. (1 An individual’s commitments to particular goals sensitize the individual to respond to cues associated with those goals. The cues may be external or internal in the person’s own mental activity. The responses may take the form of noticing the cues, storing them in memory, having thoughts or dream segments related to them, and/or taking action. Noticing may be conscious or not. Goals may be any desired endpoint of a behavioral sequence, including finding out more about something, i.e., exploring possible goals, such as job possibilities or personal relationships. (2 Such responses are accompanied and perhaps preceded by protoemotional activity or full emotional arousal, the amplitude of which determines the likelihood of response and is related to the value placed on the goal. (3 When the individual is in a situation conducive to making progress toward attaining the goal, the response to goal cues takes the form of actions or operant mental acts that advance the goal pursuit. (4 When circumstances are unfavorable for goal-directed operant behavior, the response remains purely mental, as in mind-wandering and dreaming, but still reflects the content of the goal pursuit or associated content. (5 Respondent responses such as mind-wandering are more likely when the individual is mentally unoccupied with ongoing tasks and less likely the more that is at stake in the ongoing task. The probability of respondent thought is highest during relaxed periods, when the brain’s default-mode network dominates, or during sleep. The article briefly summarizes neurocognitive findings that relate to mind-wandering and evidence regarding adverse effects of mind-wandering on task performance as well as evidence suggesting adaptive functions in regard to creative problem-solving, planning, resisting delay discounting, and

  2. Plant specific basic principle simulator as a first step to plant specific full scope simulator

    International Nuclear Information System (INIS)

    Krajnc, B.; Pribozic, F.; Novsak, M.

    1996-01-01

    Nuklearna Elektrarna Krsko (NEK) decided to enhance the quality and scope of initial training of NEK technical personnel, mainly in so called Phase 1 and 2 of training for licensed personnel. This training is a prerequisite for further training on the full scope simulator for future operators and is also given to larger number of engineers, working in different important areas where thorough knowledge of nuclear technology and plant systems is required. Due to that it was decided that plant specific Basis Principle Simulators (BPS) should be developed. The other important reason for such decision was an indication that NEK specific full scope simulator will have to be purchased. Based on that it was concluded that BPS should serve as a good opportunity to learn about the state of the art approaches in the modeling area, to see in which direction development of software in conjunction with state of the art hardware is going and in particular to the extent possible verify the existence of required plant documentation in support BPS and later plant specific full scope simulator. In this paper the scope of NEK BPS simulation, experience in initial data gathering, experience with know-how transfer based on direct involvement of NEK and Izobrazevalni Center za Jedrsko Tehnnologijo (ICJT) personnel in modeling of instrumentation and control will be presented. Lessons learned, particularly in light of coming project for NEK full scope simulator, will also be addressed. The future use of the BPS in the NEK training programs will be described. It can be concluded that due to very complex technology, phase approaches in training of key NEK technical personnel, the development of NEK plant specific BPS is justifiable, regardless of the fact that NEK will also obtain specific full scope simulator. It has to be pointed out that BPS can not be supplement for plant specific full scope simulator, due to number of reasons discussed in the paper. (author)

  3. PET/TAC: Basic principles, physiological variants and artifacts; PET/TAC: Generalidades, variantes fisiologicas y artefactos

    Energy Technology Data Exchange (ETDEWEB)

    Jimenez V, A.M. [Especialista en Medicina Nuclear, Profa. Depto. Radiologia de la Facultad de Medicina, Universidad Complutense de Madrid, Madrid (Spain)

    2007-07-01

    This presentation is about the basic principles, physiologic variants and devices that work in the PET/TAC technique. Next the conclusions obtained in the same one are presented: For a correct evaluation of the PET/TAC images with FDG is necessary the knowledge of the image acquisition technique, as well as of the physiologic distribution of the FDG, variants of the normality, benign causes of captation and more frequent devices. The introduction of this hybrid procedure allows the correct anatomical localization and identification of the deposits of FDG largely avoiding false or doubtful interpretations, but it can also originate not specific devices existent in the conventional PET. The previous knowledge of the possible devices will make possible in certain cases its elimination and in other its identification avoiding incorrect interpretations. (Author)

  4. Basic Knowledge for Market Principle: Approaches to the Price Coordination Mechanism by Using Optimization Theory and Algorithm

    Science.gov (United States)

    Aiyoshi, Eitaro; Masuda, Kazuaki

    On the basis of market fundamentalism, new types of social systems with the market mechanism such as electricity trading markets and carbon dioxide (CO2) emission trading markets have been developed. However, there are few textbooks in science and technology which present the explanation that Lagrange multipliers can be interpreted as market prices. This tutorial paper explains that (1) the steepest descent method for dual problems in optimization, and (2) Gauss-Seidel method for solving the stationary conditions of Lagrange problems with market principles, can formulate the mechanism of market pricing, which works even in the information-oriented modern society. The authors expect readers to acquire basic knowledge on optimization theory and algorithms related to economics and to utilize them for designing the mechanism of more complicated markets.

  5. RENEWAL OF BASIC LAWS AND PRINCIPLES FOR POLAR CONTINUUM THEORIES (Ⅵ)-CONSERVATION LAWS OF MASS AND INERTIA

    Institute of Scientific and Technical Information of China (English)

    戴安民

    2003-01-01

    The purpose is to reestablish the coupled conservation laws, the local conservation equations and the jump conditions of mass and inertia for polar continuum theories. In this connection the new material derivatives of the deformation gradient, the line element, the surface element and the volume element were derived and the generalized Reynolds transport theorem was presented. Combining these conservation laws of mass and inertia with the balance laws of momentum, angular momentum and energy derived in our previous papers of this series, a rather complete system of coupled basic laws and principles for polar continuum theories is constituted on the whole. From this system the coupled nonlocal balance equations of mass, inertia, momentum, angular momentum and energy may be obtained by the usual localization.

  6. Formulation of basic principles for innovative combustion and work processes; Projekt Erarbeitung von Grundlagen fuer innovative Brennverfahren und motorische Arbeitsprozesse

    Energy Technology Data Exchange (ETDEWEB)

    Barroso, G.; Escher, A.; Boulouchos, K. [Eidgenoessische Technische Hochschule (ETH), Labor fuer Aerothermochemie und Verbrennungssysteme IET, ETH-Zentrum, Zuerich (Switzerland)

    2004-07-01

    This yearly report for 2004 presents a review of work being done on behalf of the Swiss Federal Office of Energy (SFOE) at the Laboratory for Aero-thermochemistry and Combustion Systems at the Federal Institute of Technology ETH in Zurich, Switzerland, on the development of basic principles for innovative combustion and work processes. A successfully-used approach involving reaction-path analysis, heat-release analysis and the optimisation of bio-inspired algorithms is discussed. Experimental investigations made using the high-pressure, high-temperature cell at the ETH are described and initial results are discussed. The commissioning of a one-stroke Rapid Compression Machine EHT and the results of initial experiments are presented.

  7. Basic Economic Principles

    Science.gov (United States)

    Tideman, T. N.

    1972-01-01

    An economic approach to design efficient transportation systems involves maximizing an objective function that reflects both goals and costs. A demand curve can be derived by finding the quantities of a good that solve the maximization problem as one varies the price of that commodity, holding income and the prices of all other goods constant. A supply curve is derived by applying the idea of profit maximization of firms. The production function determines the relationship between input and output.

  8. Basic principles of computers

    International Nuclear Information System (INIS)

    Royal, H.D.; Parker, J.A.; Holmen, B.L.

    1988-01-01

    This chapter presents preliminary concepts of computer operations. It describes the hardware used in a nuclear medicine computer system. It discusses the software necessary for acquisition and analysis of nuclear medicine studies. The chapter outlines the integrated package of hardware and software that is necessary to perform specific functions in nuclear medicine

  9. Basic Principles of Spectroscopy

    Science.gov (United States)

    Penner, Michael H.

    Spectroscopy deals with the production, measurement, and interpretation of spectra arising from the interaction of electromagnetic radiation with matter. There are many different spectroscopic methods available for solving a wide range of analytical problems. The methods differ with respect to the species to be analyzed (such as molecular or atomic spectroscopy), the type of radiation-matter interaction to be monitored (such as absorption, emission, or diffraction), and the region of the electromagnetic spectrum used in the analysis. Spectroscopic methods are very informative and widely used for both quantitative and qualitative analyses. Spectroscopic methods based on the absorption or emission of radiation in the ultraviolet (UV), visible (Vis), infrared (IR), and radio (nuclear magnetic resonance, NMR) frequency ranges are most commonly encountered in traditional food analysis laboratories. Each of these methods is distinct in that it monitors different types of molecular or atomic transitions. The basis of these transitions is explained in the following sections.

  10. Basic Principles - Chapter 6

    Data.gov (United States)

    National Aeronautics and Space Administration — This chapter described at a very high level some of the considerations that need to be made when designing algorithms for a vehicle health management application....

  11. Basic principles of water-use licensing - Summary of economical and legal expert opinion; Grundlagen Wasserzinspolitik. Zusammenfassung oekonomisches und rechtliches Gutachten

    Energy Technology Data Exchange (ETDEWEB)

    Walter Ott, O.; Staub, C. [Econcept AG, Zuerich (Switzerland); Leimbacher, J. [Dr. Joerg Leimbacher, Koenizstrasse 43, Berne (Switzerland)

    2008-07-01

    This final report for the Swiss Federal Office of Energy (SFOE) examines the basic principles behind the definition of monetary compensation for the use of water resources in Switzerland for power generation. The basic aims of such compensation are briefly discussed as are the general economical and technical principles involved. Ownership of the rights pertaining to the use of water resources and the various definitions of licence fees and water taxes and their application are reviewed. Additional remuneration for water storage and appropriation is also discussed.

  12. Assessing the Efficacy of Mobile Health Apps Using the Basic Principles of Cognitive Behavioral Therapy: Systematic Review.

    Science.gov (United States)

    Rathbone, Amy Leigh; Clarry, Laura; Prescott, Julie

    2017-11-28

    Cognitive behavioral therapy (CBT) in its basic principle has developed itself as a stand-alone, substantial method of therapy. With effective application in therapy for a range of mental health issues, the spread of CBT methods to Web-based therapy sources is evident. The development of mobile phone apps using CBT principles is increasing within the research area. Despite the move to Web-based methods of therapy, it is argued that these methods lack the same efficacy of face-to-face therapy sessions. The aim of this review was to assess extent research findings with regard to the effectiveness of CBT-related mobile health (mHealth) apps. By assessing only studies employing a randomized controlled trial design, the review aimed to determine app efficacy within the highly regarded method of investigation. A comprehensive literature search was conducted across several databases. Search results were filtered, and results were subject to strict inclusion and exclusion criteria because of the nature of the review. Where possible, analysis of effect size was calculated and results reported. A total of 8 studies investigating the effectiveness of mHealth CBT-related apps across a range of mental health issues were reviewed. Three studies used the app against a control group, and 5 studies used the app intervention against another form of treatment or intervention. A range of effect sizes were seen across all included studies (d=-0.13 to 1.83; 0.03-1.44), with the largest effects often being seen when comparing the data from pre- to posttest for the app engaged group. The studies reviewed support the use of mHealth apps containing CBT principles for a range of mental health issues. However, the effectiveness over longer time periods should be assessed. Researchers and professionals should seek to collaborate effectively when creating new apps to enhance their effectiveness as a treatment for the general public. ©Amy Leigh Rathbone, Laura Clarry, Julie Prescott

  13. On the foundations of special theory of relativity - II. (The principle of covariance and a basic inertial frame)

    International Nuclear Information System (INIS)

    Gulati, S.P.; Gulati, S.

    1979-01-01

    An attempt has been made to replace the principle of relativity with the principle of covariance. This amounts to modification of the theory of relativity based on the two postulates (i) the principle of covariance and (ii) the light principle. Some of the fundamental results and the laws of relativistic mechanics, electromagnetodynamics and quantum mechanics are re-examined. The principle of invariance is questioned. (A.K.)

  14. Preanalytical quality improvement – in quality we trust

    OpenAIRE

    Lippi, G; Salvagno, GL; Lima-Oliveira, G; Funk-Adcock, DM; Guidi, GC; Favaloro, EJ; Biljak Radišić, V; Božičević, S; Vučić Lovrenčić, M; Milinković, N; Šumarac, Z; Ignjatović, S; Majkić-Singh, N; Trujillo Arribas, E; Camacho Martinez, P

    2013-01-01

    Background The preanalytical phase represents the major source of variability in laboratory diagnostics. Our aim was to assess to what extent underfilling of primary blood tubes may impact upon routine coagulation testing. Materials and methods: Blood was drawn by syringe from 21 healthy volunteers and 6 patients on warfarin therapy, and immediately transferred into 3.6 mL vacuum tubes containing 3.2% sodium citrate (Terumo Europe N.V., Leuven, Belgium). All tubes were filled using standardiz...

  15. "Sickle cell anemia: tracking down a mutation": an interactive learning laboratory that communicates basic principles of genetics and cellular biology.

    Science.gov (United States)

    Jarrett, Kevin; Williams, Mary; Horn, Spencer; Radford, David; Wyss, J Michael

    2016-03-01

    "Sickle cell anemia: tracking down a mutation" is a full-day, inquiry-based, biology experience for high school students enrolled in genetics or advanced biology courses. In the experience, students use restriction endonuclease digestion, cellulose acetate gel electrophoresis, and microscopy to discover which of three putative patients have the sickle cell genotype/phenotype using DNA and blood samples from wild-type and transgenic mice that carry a sickle cell mutation. The inquiry-based, problem-solving approach facilitates the students' understanding of the basic concepts of genetics and cellular and molecular biology and provides experience with contemporary tools of biotechnology. It also leads to students' appreciation of the causes and consequences of this genetic disease, which is relatively common in individuals of African descent, and increases their understanding of the first principles of genetics. This protocol provides optimal learning when led by well-trained facilitators (including the classroom teacher) and carried out in small groups (6:1 student-to-teacher ratio). This high-quality experience can be offered to a large number of students at a relatively low cost, and it is especially effective in collaboration with a local science museum and/or university. Over the past 15 yr, >12,000 students have completed this inquiry-based learning experience and demonstrated a consistent, substantial increase in their understanding of the disease and genetics in general. Copyright © 2016 The American Physiological Society.

  16. The stem-cell application in ischemic heart disease: Basic principles, specifics and practical experience from clinical studies

    Directory of Open Access Journals (Sweden)

    Banović Marko

    2015-01-01

    Full Text Available Longer life duration, different clinical presentations of coronary disease, as well as high incidence of comorbidity in patients with ischemic heart disease have led to an increase in the incidence of ischemic heart failure. Despite numerous and new treatment methods that act on different pathophysiological mechanisms that cause heart failure, and whose aim is to slowdown or stop the progression of this devastating disease, morbidity and mortality in these patients remain high. These facts have firstly led to the introduction of the experimental, and then clinical studies with the application of stem cells in patients with ischemic heart disease. Previous studies have shown that the application of stem cells is a feasible and safe method in patients with acute coronary syndrome, as well as in patients with chronic ischemic cardiomyopathy, but the efficacy of these methods in both of the abovementioned clinical syndromes has yet to be established. This review paper outlines the basic principles of treatment of ischemic heart disease with stem cells, as well as the experience and knowledge gained in previous clinical studies.

  17. Preanalytical Biases in the Measurement of Human Blood Sphingolipids

    Directory of Open Access Journals (Sweden)

    Robert Brunkhorst

    2018-05-01

    Full Text Available Dysregulation of blood sphingolipids is an emerging topic in clinical science. The objective of this study was to determine preanalytical biases that typically occur in clinical and translational studies and that influence measured blood sphingolipid levels. Therefore, we collected blood samples from four healthy male volunteers to investigate the effect of storage conditions (time, temperature, long-term storage, freeze–thaw cycles, blood drawing (venous or arterial sampling, prolonged venous compression, and sample preparation (centrifugation, freezing on sphingolipid levels measured by LC-MS/MS. Our data show that sphingosine 1-phosphate (S1P and sphinganine 1-phosphate (SA1P were upregulated in whole blood samples in a time- and temperature-dependent manner. Increased centrifugation at higher speeds led to lower amounts of S1P and SA1P. All other preanalytical biases did not significantly alter the amounts of S1P and SA1P. Further, in almost all settings, we did not detect differences in (dihydroceramide levels. In summary, besides time-, temperature-, and centrifugation-dependent changes in S1P and SA1P levels, sphingolipids in blood remained stable under practically relevant preanalytical conditions.

  18. Opportunities to Learn in School and at Home: How can they predict students' understanding of basic science concepts and principles?

    Science.gov (United States)

    Wang, Su; Liu, Xiufeng; Zhao, Yandong

    2012-09-01

    As the breadth and depth of economic reforms increase in China, growing attention is being paid to equalities in opportunities to learn science by students of various backgrounds. In early 2009, the Chinese Ministry of Education and Ministry of Science and Technology jointly sponsored a national survey of urban eighth-grade students' science literacy along with their family and school backgrounds. The present study focused on students' understanding of basic science concepts and principles (BSCP), a subset of science literacy. The sample analyzed included 3,031 students from 109 randomly selected classes/schools. Correlation analysis, one-way analysis of variance, and two-level linear regression were conducted. The results showed that having a refrigerator, internet, more books, parents purchasing books and magazines related to school work, higher father's education level, and parents' higher expectation of the education level of their child significantly predicted higher BSCP scores; having siblings at home, owning an apartment, and frequently contacting teachers about the child significantly predicted lower BSCP scores. At the school level, the results showed that being in the first-tier or key schools, having school libraries, science popularization galleries, computer labs, adequate equipment for teaching, special budget for teacher training, special budget for science equipment, and mutual trust between teachers and students significantly predicated higher BSCP scores; and having science and technology rooms, offering science and technology interest clubs, special budget for science curriculum development, and special budget for science social practice activities significantly predicted lower BSCP scores. The implications of the above findings are discussed.

  19. Control of (pre-analytical aspects in immunoassay measurements of metabolic hormones in rodents

    Directory of Open Access Journals (Sweden)

    Maximilian Bielohuby

    2018-04-01

    Full Text Available The measurement of circulating hormones by immunoassay remains a cornerstone in preclinical endocrine research. For scientists conducting and interpreting immunoassay measurements of rodent samples, the paramount aim usually is to obtain reliable and meaningful measurement data in order to draw conclusions on biological processes. However, the biological variability between samples is not the only variable affecting the readout of an immunoassay measurement and a considerable amount of unwanted or unintended variability can be quickly introduced during the pre-analytical and analytical phase. This review aims to increase the awareness for the factors ‘pre-analytical’ and ‘analytical’ variability particularly in the context of immunoassay measurement of circulating metabolic hormones in rodent samples. In addition, guidance is provided how to gain control over these variables and how to avoid common pitfalls associated with sample collection, processing, storage and measurement. Furthermore, recommendations are given on how to perform a basic validation of novel single and multiplex immunoassays for the measurement of metabolic hormones in rodents. Finally, practical examples from immunoassay measurements of plasma insulin in mice address the factors ‘sampling site and inhalation anesthesia’ as frequent sources of introducing an unwanted variability during the pre-analytical phase. The knowledge about the influence of both types of variability on the immunoassay measurement of circulating hormones as well as strategies to control these variables are crucial, on the one hand, for planning and realization of metabolic rodent studies and, on the other hand, for the generation and interpretation of meaningful immunoassay data from rodent samples.

  20. How to conduct External Quality Assessment Schemes for the pre-analytical phase?

    Science.gov (United States)

    Kristensen, Gunn B B; Aakre, Kristin Moberg; Kristoffersen, Ann Helen; Sandberg, Sverre

    2014-01-01

    In laboratory medicine, several studies have described the most frequent errors in the different phases of the total testing process, and a large proportion of these errors occur in the pre-analytical phase. Schemes for registration of errors and subsequent feedback to the participants have been conducted for decades concerning the analytical phase by External Quality Assessment (EQA) organizations operating in most countries. The aim of the paper is to present an overview of different types of EQA schemes for the pre-analytical phase, and give examples of some existing schemes. So far, very few EQA organizations have focused on the pre-analytical phase, and most EQA organizations do not offer pre-analytical EQA schemes (EQAS). It is more difficult to perform and standardize pre-analytical EQAS and also, accreditation bodies do not ask the laboratories for results from such schemes. However, some ongoing EQA programs for the pre-analytical phase do exist, and some examples are given in this paper. The methods used can be divided into three different types; collecting information about pre-analytical laboratory procedures, circulating real samples to collect information about interferences that might affect the measurement procedure, or register actual laboratory errors and relate these to quality indicators. These three types have different focus and different challenges regarding implementation, and a combination of the three is probably necessary to be able to detect and monitor the wide range of errors occurring in the pre-analytical phase.

  1. Monitoring and reporting of preanalytical errors in laboratory medicine: the UK situation.

    Science.gov (United States)

    Cornes, Michael P; Atherton, Jennifer; Pourmahram, Ghazaleh; Borthwick, Hazel; Kyle, Betty; West, Jamie; Costelloe, Seán J

    2016-03-01

    Most errors in the clinical laboratory occur in the preanalytical phase. This study aimed to comprehensively describe the prevalence and nature of preanalytical quality monitoring practices in UK clinical laboratories. A survey was sent on behalf of the Association for Clinical Biochemistry and Laboratory Medicine Preanalytical Working Group (ACB-WG-PA) to all heads of department of clinical laboratories in the UK. The survey captured data on the analytical platform and Laboratory Information Management System in use; which preanalytical errors were recorded and how they were classified and gauged interest in an external quality assurance scheme for preanalytical errors. Of the 157 laboratories asked to participate, responses were received from 104 (66.2%). Laboratory error rates were recorded per number of specimens, rather than per number of requests in 51% of respondents. Aside from serum indices for haemolysis, icterus and lipaemia, which were measured in 80% of laboratories, the most common errors recorded were booking-in errors (70.1%) and sample mislabelling (56.9%) in laboratories who record preanalytical errors. Of the laboratories surveyed, 95.9% expressed an interest in guidance on recording preanalytical error and 91.8% expressed interest in an external quality assurance scheme. This survey observes a wide variation in the definition, repertoire and collection methods for preanalytical errors in the UK. Data indicate there is a lot of interest in improving preanalytical data collection. The ACB-WG-PA aims to produce guidance and support for laboratories to standardize preanalytical data collection and to help establish and validate an external quality assurance scheme for interlaboratory comparison. © The Author(s) 2015.

  2. On the Basic Principles of Creating a Regulatory Framework for Strategic Planning of Innovative Development of the Russian Economy

    Directory of Open Access Journals (Sweden)

    Yu. V. Mishin

    2017-01-01

    Full Text Available Purpose: the aim of the work is to substantiate methodological approaches to create the most important component of strategic planning – its regulatory framework. The relevance of the chosen topic of this article is due to the fact that in modern conditions, strategic planning is an important tool for ensuring sustainable development and increasing the competitiveness of the domestic economy. It allows you to identify the most important and priority areas of activity, distribute and ensure the effective use of available limited labor, material and, most importantly, financial resources. Today, domestic strategic planning has a number of significant shortcomings, the main one of which, in our opinion, is the lack of reliable initial information for forecasting and analytical calculations. Methods: the methodological basis is the dialectical method of scientific cognition, the systemic and institutional approach to building an effective information base of strategic planning. In the course of research of the current state and level of industrial rationing, methods of analysis and synthesis, comparisons and analogies were used. As a methodological basis of this article, regulatory legal documents were used in the field of strategic planning in Russia, as well as methodological documents that previously operated in the USSR on the regulation of resource consumption. Results: the result of the work are the goals, tasks and requirements for the strategic planning base proposed by the author. The relationship of production rationing with the basic principles of strategic planning is shown - balance and consistency in priorities, goals, objectives, activities, resources and timing; The effectiveness of methods and methods for achieving goals with the least expenditure of resources used. The possibility of the existence of a standardization of labor costs, the consumption of material and production resources, regardless of the form of ownership of the

  3. Basic Principles of Creation of Topometrical Cards of Beam Therapy in the Cases of High-grade Malignant Supratentorial Gliomas

    International Nuclear Information System (INIS)

    Liepa, Z.; Platkajis, A.; Apskalne, D.

    2007-01-01

    Background. High-grade malignant supratentorial gliomas: anaplastic astrocytomas (AA), anaplastic oligodendrogliomas (AO), anaplastic oligoatrocitomas (AOA), anaplastic ependimomas (AE), glioblastomas (GB) and other less occasional forms of gliomas are approximately 1,82% of all cases of malignant tumors. Life expectancy for such patients still is very low, for several forms of tumors -12-18 months. High-grade malignant gliomas need for combined approach, and one part of such approach is beam therapy. For reaching qualitative results of beam therapy, method of topometrical planning of beam therapy is crucial, because it allow planning therapy due to anatomic features of every patient. The aim of work was comparison of basic principles of creation of 2-dimensional (2D) and 3-dimensional (3D) topometrical cards of beam therapy. Material and methods. In the process of research, analyse of creation of 2D and 3D cards for patients in period 2000-2005 were made. For creation of 2D cards pelviometer, conturometer of head (Picture 1), pictures of tests of brains in the biggest cross - section of tumor (Picture 2) were used. For creation 3D cards computertomography LightSpeed Rt, which is suitable for topometry (Picture 3), planning system of 3D reconstruction ECLIPSE (Picture 4), 3D reconstruction by data from pre - surgery and/or after - surgery tests of brain (Picture 5), and matching in format of DICOM (Picture 6) were used. In this research 214 patients with supratentorial malign gliomas were covered (Table 1,2). Results. In 98 cases 2D topometrical cards were made, which allows creating only two contrary areas of entry of beams or two areas of entry under angle (Picture 7, 8). In 55 cases in 2D topographic cards two contrary areas of entry were made and in 43 cases plan of beam therapy with areas of entry under angle were made. 3D cards anatomic features of patient as well as location of critical organs were taken into account (picture 10). In case of 3D the number of

  4. The role of European Federation of Clinical Chemistry and Laboratory Medicine Working Group for Preanalytical Phase in standardization and harmonization of the preanalytical phase in Europe

    DEFF Research Database (Denmark)

    Cornes, Michael P; Church, Stephen; van Dongen-Lases, Edmée

    2016-01-01

    Patient safety is a leading challenge in healthcare and from the laboratory perspective it is now well established that preanalytical errors are the major contributor to the overall rate of diagnostic and therapeutic errors. To address this, the European Federation of Clinical Chemistry and Labor......Patient safety is a leading challenge in healthcare and from the laboratory perspective it is now well established that preanalytical errors are the major contributor to the overall rate of diagnostic and therapeutic errors. To address this, the European Federation of Clinical Chemistry...... and Laboratory Medicine Working Group for Preanalytical Phase (EFLM WG-PRE) was established to lead in standardization and harmonization of preanalytical policies and practices at a European level. One of the key activities of the WG-PRE is the organization of the biennial EFLM-BD conference on the preanalytical...... summarises the work that has and will be done in these areas. The goal of this initiative is to ensure the EFLM WG-PRE produces work that meets the needs of the European laboratory medicine community. Progress made in the identified areas will be updated at the next preanalytical phase conference and show...

  5. Pre-analytic process control: projecting a quality image.

    Science.gov (United States)

    Serafin, Mark D

    2006-09-26

    Within the health-care system, the term "ancillary department" often describes the laboratory. Thus, laboratories may find it difficult to define their image and with it, customer perception of department quality. Regulatory requirements give laboratories who so desire an elegant way to address image and perception issues--a comprehensive pre-analytic system solution. Since large laboratories use such systems--laboratory service manuals--I describe and illustrate the process for the benefit of smaller facilities. There exist resources to help even small laboratories produce a professional service manual--an elegant solution to image and customer perception of quality.

  6. Which Basic Rules Underlie Social Judgments? Agency Follows a Zero-Sum Principle and Communion Follows a Non-Zero-Sum Principle.

    Science.gov (United States)

    Dufner, Michael; Leising, Daniel; Gebauer, Jochen E

    2016-05-01

    How are people who generally see others positively evaluated themselves? We propose that the answer to this question crucially hinges on the content domain: We hypothesize that Agency follows a "zero-sum principle" and therefore people who see others ashighin Agency are perceived aslowin Agency themselves. In contrast, we hypothesize that Communion follows a "non-zero-sum principle" and therefore people who see others ashighin Communion are perceived ashighin Communion themselves. We tested these hypotheses in a round-robin and a half-block study. Perceiving others as agentic was indeed linked to being perceived as low in Agency. To the contrary, perceiving others as communal was linked to being perceived as high in Communion, but only when people directly interacted with each other. These results help to clarify the nature of Agency and Communion and offer explanations for divergent findings in the literature. © 2016 by the Society for Personality and Social Psychology, Inc.

  7. Water System Adaptation to Hydrological Changes: Module 10, Basic Principles of Incorporating Adaptation Science into Hydrologic Planning and Design

    Science.gov (United States)

    This course will introduce students to the fundamental principles of water system adaptation to hydrological changes, with emphasis on data analysis and interpretation, technical planning, and computational modeling. Starting with real-world scenarios and adaptation needs, the co...

  8. Adopting Basic Principles of the United Nations Academic Impact Initiative (UNAI: Can Cultural Differences Be Predicted from Value Orientations and Globalization?

    Directory of Open Access Journals (Sweden)

    Andrea Nechtelberger

    2017-11-01

    Full Text Available The United Nations Academic Impact (UNAI Initiative has set forth 10 Basic Principles for higher education. In the present study, a 10 item self-report questionnaire measuring personal endorsement of these principles has been tested by self-report questionnaires with university and post-graduate students from Austria, China, Cyprus, India, Nigeria, and Slovakia (total N = 976, N = 627 female, mean age 24.7 years, s = 5.7. Starting from the assumptions of Moral Foundations Theory (MFT, we expected that personal attitudes toward the UNAI Basic Principles would be predicted by endorsement of various moral foundations as suggested by MFT and by the individual's degree of globalization. Whereas for the Austrian, Cypriot, and Nigerian sub- samples this assumption was largely confirmed, for the Chinese, Indian, and Slovak sub- samples only small amounts of the variance could be explained by regression models. All six sub-samples differed substantially with regard to their overall questionnaire responses: by five discriminant functions 83.6% of participants were classified correctly. We conclude that implementation of UNAI principles should adhere closely to the cultural requirements of the respective society and, where necessary should be accompanied by thorough informational campaigns about UN educational goals.

  9. Adopting Basic Principles of the United Nations Academic Impact Initiative (UNAI): Can Cultural Differences Be Predicted from Value Orientations and Globalization?

    Science.gov (United States)

    Nechtelberger, Andrea; Renner, Walter; Nechtelberger, Martin; Supeková, Soňa Chovanová; Hadjimarkou, Maria; Offurum, Chino; Ramalingam, Panchalan; Senft, Birgit; Redfern, Kylie

    2017-01-01

    The United Nations Academic Impact (UNAI) Initiative has set forth 10 Basic Principles for higher education. In the present study, a 10 item self-report questionnaire measuring personal endorsement of these principles has been tested by self-report questionnaires with university and post-graduate students from Austria, China, Cyprus, India, Nigeria, and Slovakia (total N = 976, N = 627 female, mean age 24.7 years, s = 5.7). Starting from the assumptions of Moral Foundations Theory (MFT), we expected that personal attitudes toward the UNAI Basic Principles would be predicted by endorsement of various moral foundations as suggested by MFT and by the individual's degree of globalization. Whereas for the Austrian, Cypriot, and Nigerian sub- samples this assumption was largely confirmed, for the Chinese, Indian, and Slovak sub- samples only small amounts of the variance could be explained by regression models. All six sub-samples differed substantially with regard to their overall questionnaire responses: by five discriminant functions 83.6% of participants were classified correctly. We conclude that implementation of UNAI principles should adhere closely to the cultural requirements of the respective society and, where necessary should be accompanied by thorough informational campaigns about UN educational goals.

  10. Adopting Basic Principles of the United Nations Academic Impact Initiative (UNAI): Can Cultural Differences Be Predicted from Value Orientations and Globalization?

    Science.gov (United States)

    Nechtelberger, Andrea; Renner, Walter; Nechtelberger, Martin; Supeková, Soňa Chovanová; Hadjimarkou, Maria; Offurum, Chino; Ramalingam, Panchalan; Senft, Birgit; Redfern, Kylie

    2017-01-01

    The United Nations Academic Impact (UNAI) Initiative has set forth 10 Basic Principles for higher education. In the present study, a 10 item self-report questionnaire measuring personal endorsement of these principles has been tested by self-report questionnaires with university and post-graduate students from Austria, China, Cyprus, India, Nigeria, and Slovakia (total N = 976, N = 627 female, mean age 24.7 years, s = 5.7). Starting from the assumptions of Moral Foundations Theory (MFT), we expected that personal attitudes toward the UNAI Basic Principles would be predicted by endorsement of various moral foundations as suggested by MFT and by the individual's degree of globalization. Whereas for the Austrian, Cypriot, and Nigerian sub- samples this assumption was largely confirmed, for the Chinese, Indian, and Slovak sub- samples only small amounts of the variance could be explained by regression models. All six sub-samples differed substantially with regard to their overall questionnaire responses: by five discriminant functions 83.6% of participants were classified correctly. We conclude that implementation of UNAI principles should adhere closely to the cultural requirements of the respective society and, where necessary should be accompanied by thorough informational campaigns about UN educational goals. PMID:29180977

  11. Basic equations of quasiparticle-phonon model of nucleus with account of Pauli principle and phonons interactions in ground state

    International Nuclear Information System (INIS)

    Voronov, V.V.; Dang, N.D.

    1984-01-01

    the system of equations, enabling to calculate the energy and the structure of excited states, described by the wave function, containing one- and two-phon components was obtained in the framework of quasiparticlephonon model. The requirements of Pauli principle for two-phonon components and phonon correlation in the ground nucleus state are taken into account

  12. Basic Principles of Marine Diesel Engines, 8-2. Military Curriculum Materials for Vocational and Technical Education.

    Science.gov (United States)

    Ohio State Univ., Columbus. National Center for Research in Vocational Education.

    This volume of student materials for a secondary/postsecondary level course in principles of marine diesel engines is one of a number of military-developed curriculum packages selected for adaptation to vocational instruction and curriculum development in a civilian setting. The purpose of the individualized, self-paced course is to acquaint…

  13. Importance and Impact of Preanalytical Variables on Alzheimer Disease Biomarker Concentrations in Cerebrospinal Fluid

    NARCIS (Netherlands)

    Le Bastard, Nathalie; De Deyn, Peter Paul; Engelborghs, Sebastiaan

    BACKGROUND: Analyses of cerebrospinal fluid (CSF) biomarkers (beta-amyloid protein, total tau protein, and hyperphosphorylated tau protein) are part of the diagnostic criteria of Alzheimer disease. Different preanalytical sample procedures contribute to variability of CSF biomarker concentrations,

  14. Does laboratory automation for the preanalytical phase improve data quality?

    Science.gov (United States)

    Lima-Oliveira, Gabriel; Lippi, Giuseppe; Salvagno, Gian Luca; Danese, Elisa; Montagnana, Martina; Brocco, Giorgio; Voi, Monica; Picheth, Geraldo; Guidi, Gian Cesare

    2013-10-01

    Our aim was to evaluate whether automation for the preanalytical phase improves data quality. Blood from 100 volunteers was collected into two vacuum tubes. One sample from each volunteer was respectively assigned to (G1) traditional processing, starting with centrifugation at 1200 g for 10 min, and (G2) the MODULAR PRE-ANALYTICALS EVO-MPA system. The routine clinical chemistry tests were performed in duplicate on the same instrument Cobas 6000 module. G1 samples were uncapped manually and immediately placed into the instrument. G2 samples were directly fed from the MPA system to the instrument without further staff intervention. At the end, (1) the G1 samples were stored for 6 h at 4 °C as prescribed in our accredited laboratory and (2) the G2 samples were stored for 6 h in the MPA output buffer. Results from G1 and G2, before and after storage, were compared. Significant increases were observed in G1 compared with G2 samples as follows: (1) before storage for alkaline phosphatase (ALP), lactate dehydrogenase (LDH), phosphate (P), magnesium (MG), iron (FE), and hemolysis index and (2) after storage for total cholesterol (COL), triglycerides (TG), total protein (TP), albumin (ALB), blood urea nitrogen (BUN), creatinine (CRE), uric acid (UA), ALP, pancreatic amylase, aspartate aminotransferase (AST), alanine aminotransferase (ALT), g-glutamyltransferase (GGT), LDH, creatine kinase (CK), calcium (CA), FE, sodium (NA), potassium (K), and hemolysis index. Moreover, significant increases were observed in (3) G1-after versus G1-before storage samples for COL, high-density lipoprotein cholesterol, TG, TP, ALB, BUN, CRE, UA, AST, ALT, GGT, LDH, P, CA, MG, FE, NA, K, and hemolysis index and (4) G2-after versus G2-before storage only for BUN, AST, LDH, P, and CA. In conclusion, our results show that the MPA system improves the quality of laboratory testing.

  15. Preanalytical errors in medical laboratories: a review of the available methodologies of data collection and analysis.

    Science.gov (United States)

    West, Jamie; Atherton, Jennifer; Costelloe, Seán J; Pourmahram, Ghazaleh; Stretton, Adam; Cornes, Michael

    2017-01-01

    Preanalytical errors have previously been shown to contribute a significant proportion of errors in laboratory processes and contribute to a number of patient safety risks. Accreditation against ISO 15189:2012 requires that laboratory Quality Management Systems consider the impact of preanalytical processes in areas such as the identification and control of non-conformances, continual improvement, internal audit and quality indicators. Previous studies have shown that there is a wide variation in the definition, repertoire and collection methods for preanalytical quality indicators. The International Federation of Clinical Chemistry Working Group on Laboratory Errors and Patient Safety has defined a number of quality indicators for the preanalytical stage, and the adoption of harmonized definitions will support interlaboratory comparisons and continual improvement. There are a variety of data collection methods, including audit, manual recording processes, incident reporting mechanisms and laboratory information systems. Quality management processes such as benchmarking, statistical process control, Pareto analysis and failure mode and effect analysis can be used to review data and should be incorporated into clinical governance mechanisms. In this paper, The Association for Clinical Biochemistry and Laboratory Medicine PreAnalytical Specialist Interest Group review the various data collection methods available. Our recommendation is the use of the laboratory information management systems as a recording mechanism for preanalytical errors as this provides the easiest and most standardized mechanism of data capture.

  16. How to interpret an unenhanced CT Brain scan. Part 1: Basic principles of Computed Tomography and relevant neuroanatomy

    Directory of Open Access Journals (Sweden)

    Thomas Osborne

    2016-08-01

    Full Text Available The aim of this article is to: Cover the basics of Computed Tomography (CT Brain imaging. Review relevant CT neuroanatomy. A CT image is produced by firing x-rays at a moving object which is then detected by an array of rotating detectors (Figure 1. The detected x-rays are then converted into a computerised signal which is used to produce a series of cross sectional images.

  17. Basic equations of the quasiparticle-phonon nuclear model with the effects due to the Pauli principle and the phonon ground state correlations

    International Nuclear Information System (INIS)

    Nguyen Dinh Dang; Voronov, V.V.

    1983-01-01

    A system of basic equations of the quasiparticle-phonon model is obtained for energies and a structure of excited states described by the wave functions containing one- and two-phonon components. The effects due to the Pauli principle for two-phonon components and the phonon ground state correlations of a spherical nucleus are taken here into account. The quantitative estimations of these effects are given by a simplified scheme. The relation between these equations with the results from other theoretical approaches is discussed

  18. Innovative curriculum: Integrating the bio-behavioral and social science principles across the LifeStages in basic science years.

    Science.gov (United States)

    Lele Mookerjee, Anuradha; Fischer, Bradford D; Cavanaugh, Susan; Rajput, Vijay

    2018-05-20

    Behavioral and social science integration in clinical practice improves health outcomes across the life stages. The medical school curriculum requires an integration of the behavioral and social science principles in early medical education. We developed and delivered a four-week course entitled "LifeStages" to the first year medical students. The learning objectives of the bio-behavioral and social science principles along with the cultural, economic, political, and ethical parameters were integrated across the lifespan in the curriculum matrix. We focused on the following major domains: Growth and Brain Development; Sexuality, Hormones and Gender; Sleep; Cognitive and Emotional Development; Mobility, Exercise, Injury and Safety; Nutrition, Diet and Lifestyle; Stress and coping skills, Domestic Violence; Substance Use Disorders; Pain, Illness and Suffering; End of Life, Ethics and Death along with Intergenerational issues and Family Dynamics. Collaboration from the clinical and biomedical science departments led to the dynamic delivery of the course learning objectives and content. The faculty developed and led a scholarly discussion, using the case of a multi-racial, multi-generational family during Active Learning Group (ALG) sessions. The assessment in the LifeStages course involved multiple assessment tools: including the holistic assessment by the faculty facilitator inside ALGs, a Team-Based Learning (TBL) exercise, multiple choice questions and Team Work Assessment during which the students had to create a clinical case on a LifeStages domain along with the facilitators guide and learning objectives.

  19. RENEWAL OF BASIC LAWS AND PRINCIPLES FOR POLAR CONTINUUM THEORIES (Ⅱ)-MICROMORPHIC CONTINUUM THEORY AND COUPLE STRESS THEORY

    Institute of Scientific and Technical Information of China (English)

    戴天民

    2003-01-01

    The purpose is to reestablish the balance laws of momentum, angular momentumand energy and to derive the corresponding local and nonlocal balance equations formicromorphic continuum mechanics and couple stress theory. The desired results formicromorphic continuum mechanics and couple stress theory are naturally obtained via directtransitions and reductions from the coupled conservation law of energy for micropolarcontinuum theory, respectively. The basic balance laws and equation s for micromorphiccontinuum mechanics and couple stress theory are constituted by combining these resultsderived here and the traditional conservation laws and equations of mass and microinertiaand the entropy inequality. The incomplete degrees of the former related continuum theoriesare clarified. Finally, some special cases are conveniently derived.

  20. Prevalence of Pre-Analytical Errors in Clinical Chemistry Diagnostic Labs in Sulaimani City of Iraqi Kurdistan

    OpenAIRE

    Najat, Dereen

    2017-01-01

    Background Laboratory testing is roughly divided into three phases: a pre-analytical phase, an analytical phase and a post-analytical phase. Most analytical errors have been attributed to the analytical phase. However, recent studies have shown that up to 70% of analytical errors reflect the pre-analytical phase. The pre-analytical phase comprises all processes from the time a laboratory request is made by a physician until the specimen is analyzed at the lab. Generally, the pre-analytical ph...

  1. uSIMPK. An Excel for Windows-based simulation program for instruction of basic pharmacokinetics principles to pharmacy students.

    Science.gov (United States)

    Brocks, Dion R

    2015-07-01

    Pharmacokinetics can be a challenging topic to teach due to the complex relationships inherent between physiological parameters, mathematical descriptors and equations, and their combined impact on shaping the blood fluid concentration vs. time curves of drugs. A computer program was developed within Microsoft Excel for Windows, designed to assist in the instruction of basic pharmacokinetics within an entry-to-practice pharmacy class environment. The program is composed of a series of spreadsheets (modules) linked by Visual Basic for Applications, intended to illustrate the relationships between pharmacokinetic and in some cases physiological parameters, doses and dose rates and the drug blood fluid concentration vs. time curves. Each module is accompanied by a simulation user's guide, prompting the user to change specific independent parameters and then observe the impact of the change(s) on the drug concentration vs. time curve and on other dependent parameters. "Slider" (or "scroll") bars can be selected to readily see the effects of repeated changes on the dependencies. Topics covered include one compartment single dose administration (iv bolus, oral, short infusion), intravenous infusion, repeated doses, renal and hepatic clearance, nonlinear elimination, two compartment model, plasma protein binding and the relationship between pharmacokinetics and drug effect. The program has been used in various forms in the classroom over a number of years, with positive ratings generally being received from students for its use in the classroom. Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.

  2. Quality Measures in Pre-Analytical Phase of Tissue Processing: Understanding Its Value in Histopathology.

    Science.gov (United States)

    Rao, Shalinee; Masilamani, Suresh; Sundaram, Sandhya; Duvuru, Prathiba; Swaminathan, Rajendiran

    2016-01-01

    Quality monitoring in histopathology unit is categorized into three phases, pre-analytical, analytical and post-analytical, to cover various steps in the entire test cycle. Review of literature on quality evaluation studies pertaining to histopathology revealed that earlier reports were mainly focused on analytical aspects with limited studies on assessment of pre-analytical phase. Pre-analytical phase encompasses several processing steps and handling of specimen/sample by multiple individuals, thus allowing enough scope for errors. Due to its critical nature and limited studies in the past to assess quality in pre-analytical phase, it deserves more attention. This study was undertaken to analyse and assess the quality parameters in pre-analytical phase in a histopathology laboratory. This was a retrospective study done on pre-analytical parameters in histopathology laboratory of a tertiary care centre on 18,626 tissue specimens received in 34 months. Registers and records were checked for efficiency and errors for pre-analytical quality variables: specimen identification, specimen in appropriate fixatives, lost specimens, daily internal quality control performance on staining, performance in inter-laboratory quality assessment program {External quality assurance program (EQAS)} and evaluation of internal non-conformities (NC) for other errors. The study revealed incorrect specimen labelling in 0.04%, 0.01% and 0.01% in 2007, 2008 and 2009 respectively. About 0.04%, 0.07% and 0.18% specimens were not sent in fixatives in 2007, 2008 and 2009 respectively. There was no incidence of specimen lost. A total of 113 non-conformities were identified out of which 92.9% belonged to the pre-analytical phase. The predominant NC (any deviation from normal standard which may generate an error and result in compromising with quality standards) identified was wrong labelling of slides. Performance in EQAS for pre-analytical phase was satisfactory in 6 of 9 cycles. A low incidence

  3. Oncology. Pt. 1. General part, epidemiology - pathogenesis - basic principles of therapy. 2. upd. ed.; Die Onkologie. T. 1. Allgemeiner Teil, Epidemiologie - Pathogenese - Grundprinzipien der Therapie

    Energy Technology Data Exchange (ETDEWEB)

    Hiddemann, Wolfgang [Muenchen Univ. Klinikum Grosshadern (Germany). Medizinische Klinik und Poliklinik III; Bartram Claus R. (eds.) [Heidelberg Univ. (Germany). Inst. fuer Humangenetik

    2010-07-01

    The book Oncology is aimed to communicate the compiled knowledge on tumor development and cancer: fundamental knowledge base, practice related know-how for diagnostics and therapy. Part 1 includes the following chapters: epidemiology and pathogenesis, basic principles of diagnostics, basic principles of therapy, complication of malign growth, tumors in the gastrointestinal tract, female genital carcinomas, kidney and urinary tract carcinomas, respiratory tract and lung carcinomas, carcinomas in the head - neck area, bone and soft tissue carcinomas, pediatric tumors, hematological neoplasm, other carcinomas. The book can be used as reference for clinical work. [German] Die gesamte Onkologie - verlaessliches Wissen fuer Ihre Kompetenz. ''Die Onkologie'' stellt sich der Herausforderung, das staendig wachsende Wissen ueber Tumorerkrankungen in seiner Gesamtheit zu vermitteln. Sowohl inhaltlich als auch didaktisch auf hoechsten Niveau: Fundiertes Grundlagenwissen zum umfassenden Nachschlagen Praxisrelevantes Know-how fuer Diagnostik und Therapie Systematischer Aufbau fuer das Verstaendnis der komplexen Zusammenhaenge Die Onkologie - eine Enzyklopaedie der modernen klinischen Tumorlehre. Angesehene Experten aus Klinik, Forschung und Praxis liefern Ihnen in Teil1 klinisches Grundlagenwissen zu den Grundprinzipien der Therapie, Epidemiologie, Aetiologie und Pathogenese, sowie zu Komplikationen des malignen Wachstums. Zum Nachschlagen und Anwenden: finden Sie alle Optionen - auch fuer Ihren schwierigsten Fall. Der uebersichtliche Aufbau und die exzellenten Abbildungen erleichtern Ihnen das schnelle Auffinden und Verstaendnis der gesuchten Informationen. Fuer den Alltag onkologisch taetiger Aerzte ist Die Onkologie ein unentbehrlicher Meilenstein. (orig.)

  4. Basic principles and applications of {sup 18}F-FDG-PET/CT in oral and maxillofacial imaging: A pictorial essay

    Energy Technology Data Exchange (ETDEWEB)

    Omami, Galal [Dept. of Oral Diagnosis and Polyclinics, Faculty of Dentistry, The Hong Kong University, Hong Kong (Hong Kong); Tamimi, Dania [BeamReaders Inc., Orlando (United States); Branstette, Barton F. [Dept. of Otolaryngology and Radiology, University of Pittsburgh School of Medicine, Pittsburgh (United States)

    2014-12-15

    A combination of positron emission tomography (PET) with 18F-labeled fluoro-2-deoxyglucose ({sup 18}F-FDG) and computed tomography ({sup 18}F-FDG-PET/CT) has increasingly become a widely used imaging modality for the diagnosis and management of head and neck cancer. On the basis of both recent literature and our professional experience, we present a set of principles with pictorial illustrations and clinical applications of FDG-PET/CT in the evaluation and management planning of squamous cell carcinoma of the oral cavity and oropharynx. We feel that this paper will be of interest and will aid the learning of oral and maxillofacial radiology trainees and practitioners.

  5. Pre-Analytical Components of Risk in Four Branches of Clinical Laboratory in Romania--Prospective Study.

    Science.gov (United States)

    David, Remona E; Dobreanu, Minodora

    2016-01-01

    Development of quality measurement principles is a strategic point for each clinical laboratory. Preexamination process is the most critical and the most difficult to be managed. The aim of this study is to identify, quantify, and monitor the nonconformities of the pre-analytical process using quality indicators that can affect the patient's health safety in four different locations of a Romanian private clinical laboratory. The study group consisted of all the analysis requests received by the departments of biochemistry, hematology, and coagulation from January through March 2015. In order to collect the pre-analytical nonconformities, we created a "Risk Budget", using the entries from the "Evidence notebook--non-conform samples" from the above mentioned departments. The laboratory established the quality indicators by means of the risk management technique in order to identify and control the sources of errors, FMEA (Failure Modes and Effects Analyses), which had been implemented and monitored for its purposes and special needs. For the assessment of the control level over the processes, the results were transformed on the Six Sigma scale, using the Westgard calculation method and being obtained in this way the frequency with which an error may occur. (https://www.westgard. com/six-sigma-calculators.htm). The obtained results prove that the quantification and monitoring of the indicators can be a control instrument for the pre-analytic activities. The calculation of the Six Sigma value adds extra information to the study because it allows the detection of the processes which need improvement (Sigma value higher than 4 represents a well controlled process). The highest rates were observed for the hemolyzed and the lipemic samples, in the department of biochemistry and hemolyzed, insufficient sample volume, or clotted samples for the department of hematology and coagulation. Significant statistical differences between laboratories participating in the study have

  6. Basic principles of legal protection with regard to the intraorganisational rights and duties of corporate bodies and their organs

    International Nuclear Information System (INIS)

    Bethge, H.

    1980-01-01

    While the foundations of disputes concerning the rights and duties of organs under constitutional order are laid down in the Basic Law and in the constitutional order, the dispute concerning the rights and duties of organs under administrative law has been elaborated by administrative jurisdiction and legal science. The author treats major aspects in form of theses. They have to be considered in the formal and material preparation of problems and in the (administrative-) procedural transfer of problems. Competencies assigned to the organs of corporate bodies may be understood as subjective public rights. To open up legally the inner circle of law would not bring about suability automatically. Special circumstances alone would lead to a suable revalorization of competence assignments, as in disputes concerning the rights and duties of organs pertaining to communities, group universities and broadcasting companies. (HSCH) [de

  7. Pre-analytic evaluation of volumetric absorptive microsampling and integration in a mass spectrometry-based metabolomics workflow.

    Science.gov (United States)

    Volani, Chiara; Caprioli, Giulia; Calderisi, Giovanni; Sigurdsson, Baldur B; Rainer, Johannes; Gentilini, Ivo; Hicks, Andrew A; Pramstaller, Peter P; Weiss, Guenter; Smarason, Sigurdur V; Paglia, Giuseppe

    2017-10-01

    Volumetric absorptive microsampling (VAMS) is a novel approach that allows single-drop (10 μL) blood collection. Integration of VAMS with mass spectrometry (MS)-based untargeted metabolomics is an attractive solution for both human and animal studies. However, to boost the use of VAMS in metabolomics, key pre-analytical questions need to be addressed. Therefore, in this work, we integrated VAMS in a MS-based untargeted metabolomics workflow and investigated pre-analytical strategies such as sample extraction procedures and metabolome stability at different storage conditions. We first evaluated the best extraction procedure for the polar metabolome and found that the highest number and amount of metabolites were recovered upon extraction with acetonitrile/water (70:30). In contrast, basic conditions (pH 9) resulted in divergent metabolite profiles mainly resulting from the extraction of intracellular metabolites originating from red blood cells. In addition, the prolonged storage of blood samples at room temperature caused significant changes in metabolome composition, but once the VAMS devices were stored at - 80 °C, the metabolome remained stable for up to 6 months. The time used for drying the sample did also affect the metabolome. In fact, some metabolites were rapidly degraded or accumulated in the sample during the first 48 h at room temperature, indicating that a longer drying step will significantly change the concentration in the sample. Graphical abstract Volumetric absorptive microsampling (VAMS) is a novel technology that allows single-drop blood collection and, in combination with mass spectrometry (MS)-based untargeted metabolomics, represents an attractive solution for both human and animal studies. In this work, we integrated VAMS in a MS-based untargeted metabolomics workflow and investigated pre-analytical strategies such as sample extraction procedures and metabolome stability at different storage conditions. The latter revealed that

  8. The rights of shareholders – basic principle of corporate governance by means of case-specific jurisprudence

    Directory of Open Access Journals (Sweden)

    Adrian Doru BÎGIOI

    2016-04-01

    Full Text Available Respecting shareholders’ rights represents one of the fundamental principles of corporate governance, underpinning the establishment of economic entities, as a form of association of individuals and / or legal entities in order to carry out profit-oriented activities. However, there are situations in which the management, the other shareholders, or even the authorities, do not respect certain shareholders’ rights, leading to a number of negative effects, such as the closing of companies. Based on these considerations, in this paper, we set as research objective to analyze the circumstances, which may affect shareholders’ rights. To meet the research objectives, we analyzed the case-specific jurisprudence published by the courts of law till 31st of December 2015. The results of the study show that the shareholders’ rights, which are not respected, include: the property right, the right to receive dividends, the right to participate and vote in the general assemblies of shareholders, the right to be elected in the governing bodies, and not the least, the most important one in accounting terms, the right to be informed.

  9. Solubilization of myofibrillar proteins in water or low ionic strength media: Classical techniques, basic principles, and novel functionalities.

    Science.gov (United States)

    Chen, Xing; Tume, Ron K; Xu, Xinglian; Zhou, Guanghong

    2017-10-13

    The qualitative characteristics of meat products are closely related to the functionality of muscle proteins. Myofibrillar proteins (MPs), comprising approximately 50% of total muscle proteins, are generally considered to be insoluble in solutions of low ionic strength ( 0.3 M) for solubilization. These soluble proteins are the ones which determine many functional properties of meat products, including emulsification and thermal gelation. In order to increase the utilization of meat and meat products, many studies have investigated the solubilization of MPs in water or low ionic strength media and determining their functionality. However, there still remains a lack of systematic information on the functional properties of MPs solubilized in this manner. Hence, this review will explore some typical techniques that have been used. The main procedures used for their solubilization, the fundamental principles and their functionalities in water (low ionic strength medium) are comprehensively discussed. In addition, advantages and disadvantages of each technique are summarized. Finally, future considerations are presented to facilitate progress in this new area and to enable water soluble muscle MPs to be utilized as novel meat ingredients in the food industry.

  10. The role of European Federation of Clinical Chemistry and Laboratory Medicine Working Group for Preanalytical Phase in standardization and harmonization of the preanalytical phase in Europe

    NARCIS (Netherlands)

    Cornes, Michael P.; Church, Stephen; van Dongen-Lases, Edmée; Grankvist, Kjell; Guimarães, João T.; Ibarz, Mercedes; Kovalevskaya, Svetlana; Kristensen, Gunn Bb; Lippi, Giuseppe; Nybo, Mads; Sprongl, Ludek; Sumarac, Zorica; Simundic, Ana-Maria

    2016-01-01

    Patient safety is a leading challenge in healthcare and from the laboratory perspective it is now well established that preanalytical errors are the major contributor to the overall rate of diagnostic and therapeutic errors. To address this, the European Federation of Clinical Chemistry and

  11. Geriatrics and radiation oncology. Pt. 1. How to identify high-risk patients and basic treatment principles

    International Nuclear Information System (INIS)

    Fels, Franziska; Kraft, Johannes W.; Grabenbauer, Gerhard G.

    2010-01-01

    Background: Until the mid of this century, 33% of the Western population will be ≥ 65 years old. The percentage of patients being ≥ 80 years old with today 5% will triple until 2050. Therefore, radiation oncologists must be familiar with special geriatric issues to meet the increasing demand for multidisciplinary cooperation and to offer useful and individual treatment concepts. Patients and Methods: This review article will provide basic data on the definition, identification and treatment of geriatric cancer patients. Results: The geriatric patient is defined by typical multimorbidity (15 items) and by age-related increased vulnerability. Best initial identification of geriatric patients will be provided by assessment including the Barthel Index evaluating self-care and activity in daily life, by the Mini-Mental Status Test that will address cognitive pattern, and by the Timed 'Up and Go' Test for evaluation of mobility. As for chemotherapy, standard treatment was associated with increased toxicity, consequently, dose modifications and supportive treatment are of special importance. Conclusion: Geriatric cancer patients need to be identified by special assessment instruments. Due to increased toxicity following chemotherapy, supportive measures seem important. Radiation treatment as a noninvasive and outpatient-based treatment remains an important and preferable option. (orig.)

  12. The frog vestibular system as a model for lesion-induced plasticity: basic neural principles and implications for posture control

    Directory of Open Access Journals (Sweden)

    Francois M Lambert

    2012-04-01

    Full Text Available Studies of behavioral consequences after unilateral labyrinthectomy have a long tradition in the quest of determining rules and limitations of the CNS to exert plastic changes that assist the recuperation from the loss of sensory inputs. Frogs were among the first animal models to illustrate general principles of regenerative capacity and reorganizational neural flexibility after a vestibular lesion. The continuous successful use of the latter animals is in part based on the easy access and identifiability of nerve branches to inner ear organs for surgical intervention, the possibility to employ whole brain preparations for in vitro studies and the limited degree of freedom of postural reflexes for quantification of behavioral impairments and subsequent improvements. Major discoveries that increased the knowledge of post-lesional reactive mechanisms in the central nervous system include alterations in vestibular commissural signal processing and activation of cooperative changes in excitatory and inhibitory inputs to disfacilitated neurons. Moreover, the observed increase of synaptic efficacy in propriospinal circuits illustrates the importance of limb proprioceptive inputs for postural recovery. Accumulated evidence suggests that the lesion-induced neural plasticity is not a goal-directed process that aims towards a meaningful restoration of vestibular reflexes but rather attempts a survival of those neurons that have lost their excitatory inputs. Accordingly, the reaction mechanism causes an improvement of some components but also a deterioration of other aspects as seen by spatio-temporally inappropriate vestibulo-motor responses, similar to the consequences of plasticity processes in various sensory systems and species. The generality of the findings indicate that frogs continue to form a highly amenable vertebrate model system for exploring molecular and physiological events during cellular and network reorganization after a loss of

  13. Platelet Function Tests: Preanalytical Variables, Clinical Utility, Advantages, and Disadvantages.

    Science.gov (United States)

    Hvas, Anne-Mette; Grove, Erik Lerkevang

    2017-01-01

    Platelet function tests are mainly used in the diagnostic work-up of platelet disorders. During the last decade, the additional use of platelet function tests to evaluate the effect of antiplatelet therapy has also emerged in an attempt to identify patients with an increased risk of arterial thrombosis. Furthermore, platelet function tests are increasingly used to measure residual effect of antiplatelet therapy prior to surgery with the aim of reducing the risk of bleeding. To a limited extend, platelet function tests are also used to evaluate hyperaggregability as a potential marker of a prothrombotic state outside the setting of antiplatelet therapy. This multifaceted use of platelet function tests and the development of simpler point-of-care tests with narrower application have increased the use of platelet function testing and also facilitated the use of platelet function tests outside the highly specialized laboratories. The present chapter describes the preanalytical variables, which should be taken into account when planning platelet function testing. Also, the most widely used platelet function tests are introduced, and their clinical utility and their relative advantages and disadvantages are discussed.

  14. Principles of macro-methodic of junior female gymnasts’ training to sport exercises for gymnastic all round competitions at specialized basic stage

    Directory of Open Access Journals (Sweden)

    V.A. Potop

    2015-08-01

    Full Text Available Purpose: working out of principles of junior female gymnasts’ macro-methodic training to sport exercises for all round competitions at stage of specialized basic training. Material: in the research 19 girl-gymnasts from reserve of combined team of Romania participated. Measurements and assessment of technical fitness at training sessions and in conditions of competitions were conducted at 120 training sessions (10 sessions a week. Results: we worked out and realized experimentally and in training sessions principles of macro-methodic training to gymnastic exercises. Macro-methodic of training is presented in structure of long-term programs of training for all round competitions. Macro-methodic is presented as combination of elements of motor, technical, didactic and technological structures of sport exercises (in the present article it was described on material of vaults of Yurchenko’s type. Conclusions: macro-methodic permits to state optimal algorithm of mastering of theoretical and practical materials at training sessions. Besides, it permits to demonstrate steady growth of sport results at competitions. With it individual-age features of junior female gymnasts, tendencies and specialists’ requirements are considered.

  15. The effect of centrifugation speed and time on pre-analytical platelet activation

    DEFF Research Database (Denmark)

    Söderström, Anna Cecilia; Nybo, Mads; Nielsen, Christian

    2016-01-01

    BACKGROUND: The results of laboratory analyses are affected by pre-analytical variables, and in particular can platelets be activated by shear handling stress and secrete granular substances. We therefore evaluated the effect of centrifugation speed and time on pre-analytical platelet activation....... METHODS: Citrate- and EDTA-anticoagulated blood from healthy volunteers were centrifuged at 80-10,000 g for 5-15 min to prepare plasma and platelet-rich plasma. Pre-analytical platelet activation was assessed by flow cytometric measurement of platelet P-selectin (CD62p) expression. Blood cell counts, mean...... of platelets expressing P-selectin in citrate- and EDTA-plasma centrifuged at 2000 g for 10 min were 43% [interquartile range (IQR), 38%-53%] and 56% (IQR, 31%-78%), respectively (p=0.82). Platelet-rich plasma prepared at 100-250 g for 10 min had significantly lower platelet P-selectin expression (11%-15%), p...

  16. Preanalytic Factors Associated With Hemolysis in Emergency Department Blood Samples.

    Science.gov (United States)

    Phelan, Michael P; Reineks, Edmunds Z; Schold, Jesse D; Hustey, Frederic M; Chamberlin, Janelle; Procop, Gary W

    2018-02-01

    - Hemolysis of emergency department blood samples is a common occurrence and has a negative impact on health care delivery. - To determine the effect of preanalytic factors (straight stick, intravenous [IV] line, needle gauge, location of blood draw, syringe versus vacuum tube use, tourniquet time) on hemolysis in emergency department blood samples. - A single 65 000-visit emergency department's electronic health record was queried for emergency department potassium results and blood draw technique for all samples obtained in calendar year 2014, resulting in 54 531 potassium results. Hemolyzed potassium was measured by hemolysis index. Comparisons of hemolysis by sampling technique were conducted by χ 2 tests. - Overall hemolysis was 10.0% (5439 of 54 531). Hemolysis among samples obtained from straight stick was significantly less than among those obtained with IV line (5.4% [33 of 615] versus 10.2% [4821 of 47 266], P < .001). For IV-placed blood draws, antecubital location had a statistically significant lower overall hemolysis compared with other locations: 7.4% (2117 of 28 786) versus 14.6% (2622 of 17 960) ( P < .001). For blood drawn with a syringe compared with vacuum, hemolysis was 13.0% (92 of 705) and 11.0% (1820 of 16 590), respectively ( P = .09, not significant). For large-gauge IV blood draws versus smaller-gauge IV lines, a lower hemolysis was also observed (9.3% [3882 of 41 571] versus 16.7% [939 of 5633]) ( P < .001). For IV-drawn blood with tourniquet time less than 60 seconds, hemolysis was 10.3% (1362 of 13 162) versus 13.9% for more than 60 seconds (532 of 3832), P < .001. - This study confirmed previous findings that straight stick and antecubital location are significantly associated with reduced hemolysis and indicated that shorter tourniquet time and larger gauge for IV draws were significantly associated with lower hemolysis.

  17. Basic hydraulics

    CERN Document Server

    Smith, P D

    1982-01-01

    BASIC Hydraulics aims to help students both to become proficient in the BASIC programming language by actually using the language in an important field of engineering and to use computing as a means of mastering the subject of hydraulics. The book begins with a summary of the technique of computing in BASIC together with comments and listing of the main commands and statements. Subsequent chapters introduce the fundamental concepts and appropriate governing equations. Topics covered include principles of fluid mechanics; flow in pipes, pipe networks and open channels; hydraulic machinery;

  18. Basic principles of radiological protection

    International Nuclear Information System (INIS)

    Pina, Jorge Luiz Soares de; Fajardo, Patricia Wieland.

    1984-07-01

    The fundamentals of radiological protection are presented. The interaction of radiation with matter and with living systems as well as radioprotection procedures and units are described. 6 refs., 7 figs., 9 tabs of radioactive wastes from nuclear medicine in Brazil are presented. 7 refs., 3 figs., 2 tabs

  19. Basic principles of ROC analysis

    International Nuclear Information System (INIS)

    Metz, C.E.

    1978-01-01

    The limitations of diagnostic accuracy as a measure of decision performance require introduction of the concepts of the sensitivity and specificity of a diagnostic test. These measures and the related indices, true positive fraction and false positive fraction, are more meaningful than accuracy, yet do not provide a unique description of diagnostic performance because they depend on the arbitrary selection of a decision threshold. The receiver operating characteristic (ROC) curve is shown to be a simple yet complete empirical description of this decision threshold effect, indicating all possible combinations of the relative frequencies of the various kinds of correct and incorrect decisions. Practical experimental techniques for measuring ROC curves are described, and the issues of case selection and curve-fitting are discussed briefly. Possible generalizations of conventional ROC analysis to account for decision performance in complex diagnostic tasks are indicated. ROC analysis is shown to be related in a direct and natural way to cost/benefit analysis of diagnostic decision making. The concepts of average diagnostic cost and average net benefit are developed and used to identify the optimal compromise among various kinds of diagnostic error. Finally, the way in which ROC analysis can be employed to optimize diagnostic strategies is suggested

  20. Basic principles of industrial radiography

    International Nuclear Information System (INIS)

    Bestbier, P.

    1983-01-01

    X-ray radiography and gamma radiography are used in industrial radiography. X-rays and gamma rays have the ability to penetrate materials and be absorbed by different densities. This characteristic makes them useful tools in industrial and medical radiography. The sources used for the generation of x-rays, gamma rays and radioisotopes are discussed. Films are one of the recording methods of x-radiography. The article also deals with advantages and disadvantages of radiography. Factors governing radiation exposure includes: the type of radiation source, material to be radiographed, distance, film, and the method for development. International specifications are also responsible for strict control over the standard of workmanship in industrial radiography

  1. Basic principles for emergency preparedness

    International Nuclear Information System (INIS)

    Hamard, J.; Bousquet-Clayeux, G.; Le Grand, J.

    1982-01-01

    The preparation of emergency plans must depend upon the type of nuclear facility and site characteristics, and will need to take into account the conditions pertaining to the time of application. The plan has to consider a wide range of accidents which might occur on the site, including these which have a very low probability of occurrence. Furthermore, the emergency countermeasures have to be taken to reduce the radiological risks to individual members of the public. This implies calculating the ''projected'' dose due to external exposure (plume and deposit) and inhalation (plume and resuspension of deposit). The calculations are based on the use of an atmospheric transfer model as simple and realistic as possible so as to cover a wide range of situations. (author)

  2. Magnetic resonance imaging: Basic principles

    International Nuclear Information System (INIS)

    Young, S.W.

    1987-01-01

    This book has been revised to reflect the past three years' technological developments and to meet the everyday needs of radiologists and clinicians who use MRI in routine practice. Among the new features are a lucid explanation of the gray scale and its significance; a complete atlas of normal MRI anatomy; and head-to-foot illustrations of pathologic MRI findings

  3. Improving quality in the preanalytical phase through innovation, on behalf of the European Federation for Clinical Chemistry and Laboratory Medicine (EFLM) Working Group for Preanalytical Phase (WG-PRE)

    DEFF Research Database (Denmark)

    Lippi, Giuseppe; Baird, Geoffrey S.; Banfi, Giuseppe

    2017-01-01

    process remain, especially in the preanalytical phase ranging from test ordering to obtaining and managing the biological specimens. The Working Group for the Preanalytical Phase (WG-PRE) of the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM) has planned many activities aimed...... at mitigating the vulnerability of the preanalytical phase, including the organization of three European meetings in the past 7 years. Hence, this collective article follows the previous three opinion papers that were published by the EFLM WGPRE on the same topic, and brings together the summaries...

  4. The knowledge and understanding of preanalytical phase among biomedicine students at the University of Zagreb

    Science.gov (United States)

    Dukic, Lora; Jokic, Anja; Kules, Josipa; Pasalic, Daria

    2016-01-01

    Introduction The educational program for health care personnel is important for reducing preanalytical errors and improving quality of laboratory test results. The aim of our study was to assess the level of knowledge on preanalytical phase in population of biomedicine students through a cross-sectional survey. Materials and methods A survey was sent to students on penultimate and final year of Faculty of Pharmacy and Biochemistry – study of medical biochemistry (FPB), Faculty of Veterinary Medicine (FVM) and School of Medicine (SM), University of Zagreb, Croatia, using the web tool SurveyMonkey. Survey was composed of demographics and 14 statements regarding the preanalytical phase of laboratory testing. Comparison of frequencies and proportions of correct answers was done with Fisher’s exact test and test of comparison of proportions, respectively. Results Study included 135 participants, median age 24 (23-40) years. Students from FPB had higher proportion of correct answers (86%) compared to students from other biomedical faculties 62%, P sample (P order of draw during blood specimen collection (P < 0.001), in comparison with students from SM and FVM. Conclusions Students from FPB are more conscious of the importance of preanalytical phase of testing in comparison with their colleagues from other biomedical faculties. No difference in knowledge between penultimate and final year of the same faculty was found. PMID:26981023

  5. Chasing the Effects of Pre-Analytical Confounders - A Multicenter Study on CSF-AD Biomarkers

    DEFF Research Database (Denmark)

    Leitão, Maria João; Baldeiras, Inês; Herukka, Sanna-Kaisa

    2015-01-01

    INTRODUCTION: Core cerebrospinal fluid (CSF) biomarkers - Aβ42, Tau, and phosphorylated Tau (pTau) - have been recently incorporated in the revised criteria for Alzheimer's disease (AD). However, their widespread clinical application lacks standardization. Pre-analytical sample handling and storage...

  6. Measuring myokines with cardiovascular functions: pre-analytical variables affecting the analytical output.

    Science.gov (United States)

    Lombardi, Giovanni; Sansoni, Veronica; Banfi, Giuseppe

    2017-08-01

    In the last few years, a growing number of molecules have been associated to an endocrine function of the skeletal muscle. Circulating myokine levels, in turn, have been associated with several pathophysiological conditions including the cardiovascular ones. However, data from different studies are often not completely comparable or even discordant. This would be due, at least in part, to the whole set of situations related to the preparation of the patient prior to blood sampling, blood sampling procedure, processing and/or store. This entire process constitutes the pre-analytical phase. The importance of the pre-analytical phase is often not considered. However, in routine diagnostics, the 70% of the errors are in this phase. Moreover, errors during the pre-analytical phase are carried over in the analytical phase and affects the final output. In research, for example, when samples are collected over a long time and by different laboratories, a standardized procedure for sample collecting and the correct procedure for sample storage are acknowledged. In this review, we discuss the pre-analytical variables potentially affecting the measurement of myokines with cardiovascular functions.

  7. Review of Pre-Analytical Errors in Oral Glucose Tolerance Testing in a Tertiary Care Hospital.

    Science.gov (United States)

    Nanda, Rachita; Patel, Suprava; Sahoo, Sibashish; Mohapatra, Eli

    2018-03-13

    The pre-pre-analytical and pre-analytical phases form a major chunk of the errors in a laboratory. The process has taken into consideration a very common procedure which is the oral glucose tolerance test to identify the pre-pre-analytical errors. Quality indicators provide evidence of quality, support accountability and help in the decision making of laboratory personnel. The aim of this research is to evaluate pre-analytical performance of the oral glucose tolerance test procedure. An observational study that was conducted overa period of three months, in the phlebotomy and accessioning unit of our laboratory using questionnaire that examined the pre-pre-analytical errors through a scoring system. The pre-analytical phase was analyzed for each sample collected as per seven quality indicators. About 25% of the population gave wrong answer with regard to the question that tested the knowledge of patient preparation. The appropriateness of test result QI-1 had the most error. Although QI-5 for sample collection had a low error rate, it is a very important indicator as any wrongly collected sample can alter the test result. Evaluating the pre-analytical and pre-pre-analytical phase is essential and must be conducted routinely on a yearly basis to identify errors and take corrective action and to facilitate their gradual introduction into routine practice.

  8. Pre-Analytical Conditions in Non-Invasive Prenatal Testing of Cell-Free Fetal RHD

    DEFF Research Database (Denmark)

    Clausen, Frederik Banch; Jakobsen, Tanja Roien; Rieneck, Klaus

    2013-01-01

    D positive fetus. Prophylaxis reduces the risk of immunization that may lead to hemolytic disease of the fetus and the newborn. The reliability of predicting the fetal RhD type depends on pre-analytical factors and assay sensitivity. We evaluated the testing setup in the Capital Region of Denmark, based...

  9. Pre-analytical factors influencing the stability of cerebrospinal fluid proteins

    DEFF Research Database (Denmark)

    Simonsen, Anja H; Bahl, Justyna M C; Danborg, Pia B

    2013-01-01

    Cerebrospinal fluid (CSF) is a potential source for new biomarkers due to its proximity to the brain. This study aimed to clarify the stability of the CSF proteome when undergoing pre-analytical factors. We investigated the effects of repeated freeze/thaw cycles, protease inhibitors and delayed s...

  10. [Quality assessment for preanalytical phase in clinical laboratory: a multicentric study].

    Science.gov (United States)

    Salinas, M; López-Garrigós, M; Yago, M; Ortuño, M; Carratala, A; Aguado, C; Díaz, J; Rodriguez-Borja, E; Chinchilla, V; Esteban, A; Laíz, B; Lorente, M Á; Uris, J

    2011-01-01

    To show the number of preanalytical sample errors in seven laboratories attending seven health departments of Valencian Community (Spain). Cross-sectional study of the number of preanlytical errors in samples obtained in primary care centers. An error is defined as a rejected specimen: any blood or urine sample, which cannot be successfully tested as it does not meet the acceptability criteria of the laboratory or if the sample is not received. We collected preanalytical errors from the tests requested for hematology, coagulation, chemistry, and urine samples. Registers were collected and indicators calculated automatically through a data warehouse and OLAP cubes software. Large differences in the results of preanalytical errors were observed between health departments. The highest percentage of errors occurred in coagulation samples, followed by urine, hematology and biochemistry. With regard to the type of error, the largest proportion of errors was due to failures of process. The high incidence of preanalytical errors and variability between health departments suggests that there is a need to standardize the drawing practice. Copyright © 2010 SECA. Published by Elsevier Espana. All rights reserved.

  11. On the constitutive laws of 1-D, two-fluid, two-phase flow models: possible mathematical forms, restrictions resulting from basic principles

    International Nuclear Information System (INIS)

    Boure, J.A.

    1981-01-01

    From both the theoretical and the practical points of view, the problem of constitutive laws is part and parcel of the modeling problem. In particular, the necessity to restore in the model, through topological laws, some of the information lost during the usual averaging process is emphasized. A new void fraction topological law is proposed. The limitations of the current assumption of uniform pressure within each phase in any cross section are also illustrated. The importance of proximity effects (neighborhood and history effects, related to characteristic lengths and times) is brought out. It results in the importance of the mathematical form of the constitutive laws. Possible mathematical forms for the transfer laws are reviewed. The last part of the paper is devoted to some restrictions, which are imposed on the transfer terms because of some basic principles: Indifference to Galilean changes of frame and to some changes of origins, second law of thermodynamics and hypothesis of local thermodynamic equilibrium, closure constraints. Practical recommendations are formulated

  12. The knowledge and understanding of preanalytical phase among biomedicine students at the University of Zagreb.

    Science.gov (United States)

    Dukic, Lora; Jokic, Anja; Kules, Josipa; Pasalic, Daria

    2016-01-01

    The educational program for health care personnel is important for reducing preanalytical errors and improving quality of laboratory test results. The aim of our study was to assess the level of knowledge on preanalytical phase in population of biomedicine students through a cross-sectional survey. A survey was sent to students on penultimate and final year of Faculty of Pharmacy and Biochemistry--study of medical biochemistry (FPB), Faculty of Veterinary Medicine (FVM) and School of Medicine (SM), University of Zagreb, Croatia, using the web tool SurveyMonkey. Survey was composed of demographics and 14 statements regarding the preanalytical phase of laboratory testing. Comparison of frequencies and proportions of correct answers was done with Fisher's exact test and test of comparison of proportions, respectively. Study included 135 participants, median age 24 (23-40) years. Students from FPB had higher proportion of correct answers (86%) compared to students from other biomedical faculties 62%, P < 0.001. Students from FPB were more conscious of the importance of specimen mixing (P = 0.027), prevalence of preanalytical errors (P = 0.001), impact of hemolysis (P = 0.032) and lipemia interferences (P = 0.010), proper choice of anticoagulants (P = 0.001), transport conditions for ammonia sample (P < 0.001) and order of draw during blood specimen collection (P < 0.001), in comparison with students from SM and FVM. Students from FPB are more conscious of the importance of preanalytical phase of testing in comparison with their colleagues from other biomedical faculties. No difference in knowledge between penultimate and final year of the same faculty was found.

  13. Pre-analytic phase in molecular biology: criticism and non-compliance management

    Directory of Open Access Journals (Sweden)

    Catia Sias

    2010-06-01

    Full Text Available Introduction: During workflow in Laboratories the most delicate and important step is pre-analytic sample treatment because it involves more than one operator of the same structure and often different health services. In fact, the biological materials used for the diagnosis should be collected, sent and properly treated before the analytic phase. Correct methods for collecting and handling biological materials, including guidelines to users of laboratory services, improve performance of Laboratory testing activity. In the pre-analytic phase the operators check sample integrity, and prepare the sample for the subsequent analytic phase: in all these steps monitoring and control of “non- compliance” is crucial. Methods: During 2007-2008 we created a “non- compliance” check-list, to monitor errors which occurred in different sectors of the preanalytic phase, particularly in the nucleic acid extraction step. These “non-compliances” are analysed to identify and to remove errors, adopting preventive and corrective proceedings. Since 2008 we have been using DNA/RNA internal controls synthesized in our Laboratory. They can be amplified by the same primers and recognized by different probes. Results: Examination of the “non compliance” check-list for molecular biology investigations shows that the percentage of urine repeat samples decreased from 17% to 2% and the percentage of stool repeat samples from 27% to 2%. Regarding use of internal controls, they allow the assessment of inhibitory factors that can prevent gene amplification. Conclusions: Monitoring “non-compliance” cases and dividing them by typology allow us identifying the most frequent causes of incorrect sample handling, as a non optimal procedure of pre-treatment, thus improving the pre-analytic phase. Therefore by monitoring the preanalytic phase we can prevent the introduction of confounding factors that may negatively influence the accuracy of results and their

  14. Basic principles of knowledge management and its application to the industrial company in tactical operations of maintenance and operational exploitation: A qualitative study

    Directory of Open Access Journals (Sweden)

    Francisco Javier Cárcel Carrasco

    2013-01-01

    Full Text Available Purpose: Although knowledge and its management is, and has been, studied in depth, particularly since the 90’s decade of the past century, especially for the strategic management, innovation, trade, or business administration, there are still many questions on how it articulates or transfers and the barriers to its management, especially when we talk about internal tactical activities that affect staff that we could call "offices", such as maintenance and industrial assembly or exploitation and conduction of the facilities. Because of the peculiarities normally seen in this kind of activity in the industrial companies, the knowledge of these people is strongly based on their experience (strong tacit component, difficult to measure and articulate, and on many occasions, however, this rupture of information-knowledge, can represent a high cost for the company (often assumed to be something inevitable due to the increase in production and service downtime, loss of efficiency, or tuning time of new staff to these areas.Design/methodology/approach: After a description of the State of the art and the basic principles of knowledge management, a qualitative study in an industrial company has been carried out, within the areas of operation and maintenance, in order to know the barriers and facilitators that such involved personnel finds in order to achieve an adequate transmission and use of that knowledge.Findings and Originality/value: Learn about barriers, facilitators and impact that offers the knowledge management in the areas of industrial maintenance, especially among professionals in offices which operate with a high degree of tacit knowledge and with a heavy dependence on the company on these professionals.Research limitations/implications: Those characteristics of a qualitative study in a particular area and within a geographical area, although it can be extrapolated to other types of companies and regional areas.Practical implications: Learn

  15. Phase contrast imaging of the breast. Basic principles and steps towards clinical implementation; Phasenkontrastbildgebung der Brust. Grundlagen und Schritte zur klinischen Implementierbarkeit

    Energy Technology Data Exchange (ETDEWEB)

    Grandl, S.; Sztrokay-Gaul, A.; Auweter, S.D.; Hellerhoff, K. [Klinikum der Ludwig-Maximilians-Universitaet Muenchen, Campus Grosshadern, Institut fuer Klinische Radiologie, Muenchen (Germany)

    2014-03-15

    Breast cancer is the most common cancer and the leading cause of cancer deaths in women worldwide. Mammography is the only imaging technique approved for nationwide breast cancer screening. Digital full field mammography has improved mammographic image quality. Nevertheless, mammography has a low positive predictive value and a low sensitivity especially in mammographically dense breasts. One of the major limitations is the inherently low contrast between healthy breast parenchyma and breast cancer. Phase contrast imaging is based on the phase shift that occurs when X-rays encounter a change in refractive index between different materials. The improved soft tissue contrast makes the technology particularly promising for breast diagnostics. The studies presented here suggest that phase contrast imaging provides additional diagnostic information both using phase contrast mammography and phase contrast computed tomography (CT). This paper provides an overview of the basic principles of the phase contrast imaging and describes recent developments towards in vivo and ex vivo phase contrast imaging of the breast. (orig.) [German] Brustkrebs ist weltweit die haeufigste Tumorerkrankung und die haeufigste Krebstodesursache der Frau. Die Mammographie ist die einzige zugelassene bildgebende Methode zur Brustkrebsfrueherkennung im Rahmen flaechendeckender Screeningprogramme. Trotz Verbesserung der Bildqualitaet und der Befundungsperformance durch die Einfuehrung der digitalen Vollfeldmammographie sind der positiv-praediktive Wert und die Sensitivitaet der Mammographie insbesondere bei mammographisch dichtem Druesenkoerper eingeschraenkt. Dies ist u. a. auf die geringen Dichteunterschiede zwischen gesundem Brustdruesengewebe und intramammaeren Malignomen zurueckzufuehren. Die Phasenkontrastbildgebung macht sich die Phasenverschiebung von Roentgenstrahlen zunutze, die an Materialgrenzen mit unterschiedlichen Brechungsindizes entsteht. Die Technik bietet einen potenziell

  16. The constitutive laws of one-dimensional, two-fluid models for two-phase flows - Possible mathematical forms - Restrictions resulting from basic principles

    International Nuclear Information System (INIS)

    Boure, Jean.

    1978-05-01

    From both the theoretical and the practical points of view, the problem of constitutive laws is a part and parcel of the modeling problem. In particular, the necessity to restore in the model, through topological laws, some of the information lost during the usual averaging process is emphasized. It is shown that the customary 'void fraction' topological law Psub(V)=Psub(L) should be proscribed whenever propagation phenomena are involved. A new void fraction topological law is proposed. The limitations of the current assumption of constant pressure within any phase in any cross section are also illustrated. The importance of proximity effects (neighborhood and history effects, related to characteristic lengths and times) is brought out. It results in the importance of the mathematical form of the constitutive laws. Various approaches to the constitutive law problem and possible mathematical forms for the transfer laws are reviewed. The simplest form (transfert terms as functions of the dependent variables only) may have some usefulness if interpretation of the results in terms of propagation phenomena is banned. A good compromise between the necessity to take proximity effects into account and to obtain a tractable set of equations is carried out when so called 'differential terms' are introduced in the transfer laws. The last part of the paper is devoted to some restrictions, which are imposed to the transfer terms because of some basic principles: indifference to Galilean changes of frame and to some changes of origins, second law of thermodynamics and assumption of local thermodynamic equilibrium, closure constraints. Practical recommendations are formulated [fr

  17. Pre-analytical issues in the haemostasis laboratory: guidance for the clinical laboratories.

    Science.gov (United States)

    Magnette, A; Chatelain, M; Chatelain, B; Ten Cate, H; Mullier, F

    2016-01-01

    Ensuring quality has become a daily requirement in laboratories. In haemostasis, even more than in other disciplines of biology, quality is determined by a pre-analytical step that encompasses all procedures, starting with the formulation of the medical question, and includes patient preparation, sample collection, handling, transportation, processing, and storage until time of analysis. This step, based on a variety of manual activities, is the most vulnerable part of the total testing process and is a major component of the reliability and validity of results in haemostasis and constitutes the most important source of erroneous or un-interpretable results. Pre-analytical errors may occur throughout the testing process and arise from unsuitable, inappropriate or wrongly handled procedures. Problems may arise during the collection of blood specimens such as misidentification of the sample, use of inadequate devices or needles, incorrect order of draw, prolonged tourniquet placing, unsuccessful attempts to locate the vein, incorrect use of additive tubes, collection of unsuitable samples for quality or quantity, inappropriate mixing of a sample, etc. Some factors can alter the result of a sample constituent after collection during transportation, preparation and storage. Laboratory errors can often have serious adverse consequences. Lack of standardized procedures for sample collection accounts for most of the errors encountered within the total testing process. They can also have clinical consequences as well as a significant impact on patient care, especially those related to specialized tests as these are often considered as "diagnostic". Controlling pre-analytical variables is critical since this has a direct influence on the quality of results and on their clinical reliability. The accurate standardization of the pre-analytical phase is of pivotal importance for achieving reliable results of coagulation tests and should reduce the side effects of the influence

  18. Inadequate programming, insufficient communication and non-compliance with the basic principles of maternal death audits in health districts in Burkina Faso: a qualitative study.

    Science.gov (United States)

    Congo, Boukaré; Sanon, Djénéba; Millogo, Tieba; Ouedraogo, Charlemagne Marie; Yaméogo, Wambi Maurice E; Meda, Ziemlé Clement; Kouanda, Seni

    2017-09-29

    Implementation of quality maternal death audits requires good programming, good communication and compliance with core principles. Studies on compliance with core principles in the conduct of maternal death audits (MDAs) exist but were conducted in urban areas, at the 2nd or 3rd level of the healthcare system, in experimental situations, or in a context of skills-building projects or technical platforms with an emphasis on the review of "near miss". This study aims to fill the gap of evidence on the implementation of MDAs in rural settings, at the first level of care and in the routine care situation in Burkina Faso. We conducted a multiple-case study, with seven cases (health districts) chosen by contrasted purposive sampling using four criteria: (i) the intra-hospital maternal mortality rates for 2013, (ii) rural versus urban location, (iii) proofs of regular conduct of maternal death audits (MDAs) as per routine health information system, and (iv) the use of district hospital versus regional hospital for reference when the first mentioned does not exist. A review of audit records and structured and semi-structured interviews with staff involved in MDAs were conducted. The survey was conducted from 27 April to 30 May of 2015. The results showed that maternal death audits (MDAs) were irregularly scheduled, mostly driven by critical events. Overall, preparing sessions, communication and the conduct of MDAs were most of the time inadequate. Confidentiality was globally respected during the clinical audit sessions. The principle of "no name, no shame, and no blame" was differently applied and anonymity was rarely preserved. Programming, communication, and compliance with the basic principles in the conduct of maternal death audits were inadequate as compared to the national standards. Identifying determinants of such shortcomings may help guide interventions to improve the quality of clinical audits. La mise en œuvre d'audits de décès maternels de qualité n

  19. Principles of project management

    Science.gov (United States)

    1982-01-01

    The basic principles of project management as practiced by NASA management personnel are presented. These principles are given as ground rules and guidelines to be used in the performance of research, development, construction or operational assignments.

  20. Pre-analytic phase in molecular biology: criticism and non-compliance management

    OpenAIRE

    Catia Sias; Loredana Aleo; Stefania Di Filippo; Marco Paterno; Anna Rosa Garbuglia; Maria Rosaria Capobianchi

    2010-01-01

    Introduction: During workflow in Laboratories the most delicate and important step is pre-analytic sample treatment because it involves more than one operator of the same structure and often different health services. In fact, the biological materials used for the diagnosis should be collected, sent and properly treated before the analytic phase. Correct methods for collecting and handling biological materials, including guidelines to users of laboratory services, improve performance of Labor...

  1. Plasma tissue inhibitor of metalloproteinases-1 as a biological marker? Pre-analytical considerations

    DEFF Research Database (Denmark)

    Lomholt, Anne Fog; Frederiksen, Camilla; Christensen, Ib Jarle

    2007-01-01

    Tissue Inhibitor of Metalloproteinases-1 (TIMP-1) may be a valuable biological marker in Colorectal Cancer (CRC). However, prospective validation of TIMP-1 as a biological marker should include a series of pre-analytical considerations. TIMP-1 is stored in platelets, which may degranulate during ...... collection and storage. The aim of this study was to evaluate the influence of platelet TIMP-1 contamination on plasma TIMP-1 levels in healthy volunteers....

  2. Role of Logic and Mentality as the Basics of Wittgenstein's Picture Theory of Language and Extracting Educational Principles and Methods According to This Theory

    Science.gov (United States)

    Heshi, Kamal Nosrati; Nasrabadi, Hassanali Bakhtiyar

    2016-01-01

    The present paper attempts to recognize principles and methods of education based on Wittgenstein's picture theory of language. This qualitative research utilized inferential analytical approach to review the related literature and extracted a set of principles and methods from his theory on picture language. Findings revealed that Wittgenstein…

  3. Biofluid infrared spectro-diagnostics: pre-analytical considerations for clinical applications.

    Science.gov (United States)

    Lovergne, L; Bouzy, P; Untereiner, V; Garnotel, R; Baker, M J; Thiéfin, G; Sockalingum, G D

    2016-06-23

    Several proof-of-concept studies on the vibrational spectroscopy of biofluids have demonstrated that the methodology has promising potential as a clinical diagnostic tool. However, these studies also show that there is a lack of a standardised protocol in sample handling and preparation prior to spectroscopic analysis. One of the most important sources of analytical errors is the pre-analytical phase. For the technique to be translated into clinics, it is clear that a very strict protocol needs to be established for such biological samples. This study focuses on some of the aspects of the pre-analytical phase in the development of the high-throughput Fourier Transform Infrared (FTIR) spectroscopy of some of the most common biofluids such as serum, plasma and bile. Pre-analytical considerations that can impact either the samples (solvents, anti-coagulants, freeze-thaw cycles…) and/or spectroscopic analysis (sample preparation such as drying, deposit methods, volumes, substrates, operators dependence…) and consequently the quality and the reproducibility of spectral data will be discussed in this report.

  4. Pre-Analytical Parameters Affecting Vascular Endothelial Growth Factor Measurement in Plasma: Identifying Confounders.

    Directory of Open Access Journals (Sweden)

    Johanna M Walz

    Full Text Available Vascular endothelial growth factor-A (VEGF-A is intensively investigated in various medical fields. However, comparing VEGF-A measurements is difficult because sample acquisition and pre-analytic procedures differ between studies. We therefore investigated which variables act as confounders of VEGF-A measurements.Following a standardized protocol, blood was taken at three clinical sites from six healthy participants (one male and one female participant at each center twice one week apart. The following pre-analytical parameters were varied in order to analyze their impact on VEGF-A measurements: analyzing center, anticoagulant (EDTA vs. PECT / CTAD, cannula (butterfly vs. neonatal, type of centrifuge (swing-out vs. fixed-angle, time before and after centrifugation, filling level (completely filled vs. half-filled tubes and analyzing method (ELISA vs. multiplex bead array. Additionally, intrapersonal variations over time and sex differences were explored. Statistical analysis was performed using a linear regression model.The following parameters were identified as statistically significant independent confounders of VEGF-A measurements: analyzing center, anticoagulant, centrifuge, analyzing method and sex of the proband. The following parameters were no significant confounders in our data set: intrapersonal variation over one week, cannula, time before and after centrifugation and filling level of collection tubes.VEGF-A measurement results can be affected significantly by the identified pre-analytical parameters. We recommend the use of CTAD anticoagulant, a standardized type of centrifuge and one central laboratory using the same analyzing method for all samples.

  5. Pre-Analytical Parameters Affecting Vascular Endothelial Growth Factor Measurement in Plasma: Identifying Confounders.

    Science.gov (United States)

    Walz, Johanna M; Boehringer, Daniel; Deissler, Heidrun L; Faerber, Lothar; Goepfert, Jens C; Heiduschka, Peter; Kleeberger, Susannah M; Klettner, Alexa; Krohne, Tim U; Schneiderhan-Marra, Nicole; Ziemssen, Focke; Stahl, Andreas

    2016-01-01

    Vascular endothelial growth factor-A (VEGF-A) is intensively investigated in various medical fields. However, comparing VEGF-A measurements is difficult because sample acquisition and pre-analytic procedures differ between studies. We therefore investigated which variables act as confounders of VEGF-A measurements. Following a standardized protocol, blood was taken at three clinical sites from six healthy participants (one male and one female participant at each center) twice one week apart. The following pre-analytical parameters were varied in order to analyze their impact on VEGF-A measurements: analyzing center, anticoagulant (EDTA vs. PECT / CTAD), cannula (butterfly vs. neonatal), type of centrifuge (swing-out vs. fixed-angle), time before and after centrifugation, filling level (completely filled vs. half-filled tubes) and analyzing method (ELISA vs. multiplex bead array). Additionally, intrapersonal variations over time and sex differences were explored. Statistical analysis was performed using a linear regression model. The following parameters were identified as statistically significant independent confounders of VEGF-A measurements: analyzing center, anticoagulant, centrifuge, analyzing method and sex of the proband. The following parameters were no significant confounders in our data set: intrapersonal variation over one week, cannula, time before and after centrifugation and filling level of collection tubes. VEGF-A measurement results can be affected significantly by the identified pre-analytical parameters. We recommend the use of CTAD anticoagulant, a standardized type of centrifuge and one central laboratory using the same analyzing method for all samples.

  6. Pre-analytical Factors Influence Accuracy of Urine Spot Iodine Assessment in Epidemiological Surveys.

    Science.gov (United States)

    Doggui, Radhouene; El Ati-Hellal, Myriam; Traissac, Pierre; El Ati, Jalila

    2018-03-26

    Urinary iodine concentration (UIC) is commonly used to assess iodine status of subjects in epidemiological surveys. As pre-analytical factors are an important source of measurement error and studies about this phase are scarce, our objective was to assess the influence of urine sampling conditions on UIC, i.e., whether the child ate breakfast or not, urine void rank of the day, and time span between last meal and urine collection. A nationwide, two-stage, stratified, cross-sectional study including 1560 children (6-12 years) was performed in 2012. UIC was determined by the Sandell-Kolthoff method. Pre-analytical factors were assessed from children's mothers by using a questionnaire. Association between iodine status and pre-analytical factors were adjusted for one another and socio-economic characteristics by multivariate linear and multinomial regression models (RPR: relative prevalence ratios). Skipping breakfast prior to morning urine sampling decreased UIC by 40 to 50 μg/L and the proportion of UIC analytical factors is a key step toward improving accuracy and comparability of survey results for assessing iodine status from spot urine samples. These recommendations have to be evaluated by future research.

  7. Impact of Educational Activities in Reducing Pre-Analytical Laboratory Errors: A quality initiative.

    Science.gov (United States)

    Al-Ghaithi, Hamed; Pathare, Anil; Al-Mamari, Sahimah; Villacrucis, Rodrigo; Fawaz, Naglaa; Alkindi, Salam

    2017-08-01

    Pre-analytic errors during diagnostic laboratory investigations can lead to increased patient morbidity and mortality. This study aimed to ascertain the effect of educational nursing activities on the incidence of pre-analytical errors resulting in non-conforming blood samples. This study was conducted between January 2008 and December 2015. All specimens received at the Haematology Laboratory of the Sultan Qaboos University Hospital, Muscat, Oman, during this period were prospectively collected and analysed. Similar data from 2007 were collected retrospectively and used as a baseline for comparison. Non-conforming samples were defined as either clotted samples, haemolysed samples, use of the wrong anticoagulant, insufficient quantities of blood collected, incorrect/lack of labelling on a sample or lack of delivery of a sample in spite of a sample request. From 2008 onwards, multiple educational training activities directed at the hospital nursing staff and nursing students primarily responsible for blood collection were implemented on a regular basis. After initiating corrective measures in 2008, a progressive reduction in the percentage of non-conforming samples was observed from 2009 onwards. Despite a 127.84% increase in the total number of specimens received, there was a significant reduction in non-conforming samples from 0.29% in 2007 to 0.07% in 2015, resulting in an improvement of 75.86% ( P educational activities directed primarily towards hospital nursing staff had a positive impact on the quality of laboratory specimens by significantly reducing pre-analytical errors.

  8. Novel Opportunities for Improving the Quality of Preanalytical Phase. A Glimpse to the Future?

    Directory of Open Access Journals (Sweden)

    Lippi Giuseppe

    2017-08-01

    Full Text Available The preanalytical phase is crucial for assuring the quality of in vitro diagnostics. The leading aspects which contribute to enhance the vulnerability of this part of the total testing process include the lack of standardization of different practices for collecting, managing, transporting and processing biological specimens, the insufficient compliance with available guidelines and the still considerable number of preventable human errors. As in heavy industry, road traffic and aeronautics, technological advancement holds great promise for decreasing the risk of medical and diagnostic errors, thus including those occurring in the extra-analytical phases of the total testing process. The aim of this article is to discuss some potentially useful technological advances, which are not yet routine practice, but may be especially suited for improving the quality of the preanalytical phase in the future. These are mainly represented by introduction of needlewielding robotic phlebotomy devices, active blood tubes, drones for biological samples transportation, innovative approaches for detecting spurious hemolysis and preanalytical errors recording software products.

  9. Preanalytical external quality assessment of the Croatian Society of Medical Biochemistry and Laboratory Medicine and CROQALM: finding undetected weak spots.

    Science.gov (United States)

    Nikolac, Nora; Krleza, Jasna Lenicek; Simundic, Ana-Maria

    2017-02-15

    The aim of this paper is to present results of first two years of preanalytical external quality assessment (EQA) in Croatia. This paper summarizes results from 6 rounds of preanalytical EQA during 2014-2016 in 161-175 Croatian laboratories (number ranged between cycles). EQA was designed as an online survey of the compliance with National recommendations for phlebotomy (NRP). Forty-seven questions in 5 categories are analyzed (materials and equipment, patient identification, patient preparation, sampling and storage). Additionally, preanalytical cases are presented. Overall performance scores (Question score (Qscore) for compliance with NRP and Case score (Cscore) for preanalytical cases) are calculated for each question/case as a proportion of laboratories with satisfactory procedure (x 100). Qscores and Cscores ≥ 70 were classified as acceptable (maximal score = 100). In investigation of compliance with NRP, acceptable Qscores were obtained for 34/47 questions. The lowest scores were observed for the availability of sterile disposable tourniquets (Qscore = 15) and safe-sharp needles (Qscore = 34), obtaining patients address as an identifier (Qscore = 21), using glycolysis inhibitor tubes for glucose concentration measurement (Qscore = 21) and verification of manufacturers declarations on temperature and time of storage (Qscore = 31). There was no statistically significant difference in overall Qscore according to different categories of phlebotomy procedures (P = 0.284). The results of preanalytical cases showed acceptable Cscore values for all cases (89-96). First two years of preanalytical EQA showed good compliance with the NRP and excellent expertise in resolving complex preanalytical issues. Major critical spots are lack of availability of safe-sharp needles, disposable tourniquets and glucose inhibitor tubes.

  10. Prevalence of Pre-Analytical Errors in Clinical Chemistry Diagnostic Labs in Sulaimani City of Iraqi Kurdistan.

    Directory of Open Access Journals (Sweden)

    Dereen Najat

    Full Text Available Laboratory testing is roughly divided into three phases: a pre-analytical phase, an analytical phase and a post-analytical phase. Most analytical errors have been attributed to the analytical phase. However, recent studies have shown that up to 70% of analytical errors reflect the pre-analytical phase. The pre-analytical phase comprises all processes from the time a laboratory request is made by a physician until the specimen is analyzed at the lab. Generally, the pre-analytical phase includes patient preparation, specimen transportation, specimen collection and storage. In the present study, we report the first comprehensive assessment of the frequency and types of pre-analytical errors at the Sulaimani diagnostic labs in Iraqi Kurdistan.Over 2 months, 5500 venous blood samples were observed in 10 public diagnostic labs of Sulaimani City. The percentages of rejected samples and types of sample inappropriateness were evaluated. The percentage of each of the following pre-analytical errors were recorded: delay in sample transportation, clotted samples, expired reagents, hemolyzed samples, samples not on ice, incorrect sample identification, insufficient sample, tube broken in centrifuge, request procedure errors, sample mix-ups, communication conflicts, misinterpreted orders, lipemic samples, contaminated samples and missed physician's request orders. The difference between the relative frequencies of errors observed in the hospitals considered was tested using a proportional Z test. In particular, the survey aimed to discover whether analytical errors were recorded and examine the types of platforms used in the selected diagnostic labs.The analysis showed a high prevalence of improper sample handling during the pre-analytical phase. In appropriate samples, the percentage error was as high as 39%. The major reasons for rejection were hemolyzed samples (9%, incorrect sample identification (8% and clotted samples (6%. Most quality control schemes

  11. Prevalence of Pre-Analytical Errors in Clinical Chemistry Diagnostic Labs in Sulaimani City of Iraqi Kurdistan.

    Science.gov (United States)

    Najat, Dereen

    2017-01-01

    Laboratory testing is roughly divided into three phases: a pre-analytical phase, an analytical phase and a post-analytical phase. Most analytical errors have been attributed to the analytical phase. However, recent studies have shown that up to 70% of analytical errors reflect the pre-analytical phase. The pre-analytical phase comprises all processes from the time a laboratory request is made by a physician until the specimen is analyzed at the lab. Generally, the pre-analytical phase includes patient preparation, specimen transportation, specimen collection and storage. In the present study, we report the first comprehensive assessment of the frequency and types of pre-analytical errors at the Sulaimani diagnostic labs in Iraqi Kurdistan. Over 2 months, 5500 venous blood samples were observed in 10 public diagnostic labs of Sulaimani City. The percentages of rejected samples and types of sample inappropriateness were evaluated. The percentage of each of the following pre-analytical errors were recorded: delay in sample transportation, clotted samples, expired reagents, hemolyzed samples, samples not on ice, incorrect sample identification, insufficient sample, tube broken in centrifuge, request procedure errors, sample mix-ups, communication conflicts, misinterpreted orders, lipemic samples, contaminated samples and missed physician's request orders. The difference between the relative frequencies of errors observed in the hospitals considered was tested using a proportional Z test. In particular, the survey aimed to discover whether analytical errors were recorded and examine the types of platforms used in the selected diagnostic labs. The analysis showed a high prevalence of improper sample handling during the pre-analytical phase. In appropriate samples, the percentage error was as high as 39%. The major reasons for rejection were hemolyzed samples (9%), incorrect sample identification (8%) and clotted samples (6%). Most quality control schemes at Sulaimani

  12. Intensive educational efforts combined with external quality assessment improve the preanalytical phase in general practitioner offices and nursing homes.

    Science.gov (United States)

    Sølvik, Una Ørvim; Bjelkarøy, Wenche Iren; Berg, Kari van den; Saga, Anne Lise; Hager, Helle Borgstrøm; Sandberg, Sverre

    2017-10-26

    Errors in the preanalytical phase in clinical laboratories affect patient safety. The aim of this study was to evaluate the effect of intensive educational efforts together with external quality assessment (EQA) of the preanalytical phase from 2013 to 2015 to improve patient identification in primary health care in Norway. In addition, routines for venous and capillary blood sampling were investigated. A preanalytical EQA was circulated in 2013 by the Norwegian Quality Improvement of Laboratory Examinations (Noklus) to general practitioner offices and nursing homes (n=2000) to obtain information about important issues to focus on before launching an intensive educational program with courses, posters and visits in 2013-2015. Preanalytical EQA surveys were further circulated in 2014 and 2015. The response rate varied between 42% and 55%. The percentages of participants asking for the patients' name and the Norwegian identification number increased from about 8% in 2013 to about 35% in 2015. The increase was similar for those participating in only one EQA survey and for those who participated in EQA surveys both in 2013 and 2015. Guidelines for venous and capillary blood sampling were not always followed. Educational efforts more than the preanalytical EQA influenced the actions and resulted in an increase in the percentages of participants that followed the guidelines for patient identification. Some aspects of blood sampling routines need improvement.

  13. Basic electronics

    CERN Document Server

    Tayal, DC

    2010-01-01

    The second edition of this book incorporates the comments and suggestions of my friends and students who have critically studied the first edition. In this edition the changes and additions have been made and subject matter has been rearranged at some places. The purpose of this text is to provide a comprehensive and up-to-date study of the principles of operation of solid state devices, their basic circuits and application of these circuits to various electronic systems, so that it can serve as a standard text not only for universities and colleges but also for technical institutes. This book

  14. PRINCIPLES OF HYDROGEOMORPHOLOGY AS A BASIC PRECONDITION FOR SOLUTION OF TERRITORIAL STRUCTURE OF UNITARY SYSTEM OF AGRICULTURAL, FOREST AND WATER MANAGEMENT

    Directory of Open Access Journals (Sweden)

    K KUDRNA

    2007-07-01

    Full Text Available In the presented work, the laws of hydrogeomorfhology have been defi ned on the principle of symmetry and invariance, which are to be respected at solution of territorial structure of Unitary System of Agricultural, Forest and Water Management (USAFWM. The principle of the solution is a dominant position of the geomorphologic formation Gh of a given sea-level altitude in the analyzed part of territory, which determines control and regulation of all components of water balance. The newly formed territory unit, delimited around the geomorphologic formation by water streams, was called a hydrogeomorphologic region of the third order (HGR-3.

  15. Integrated and Contextual Basic Science Instruction in Preclinical Education: Problem-Based Learning Experience Enriched with Brain/Mind Learning Principles

    Science.gov (United States)

    Gülpinar, Mehmet Ali; Isoglu-Alkaç, Ümmühan; Yegen, Berrak Çaglayan

    2015-01-01

    Recently, integrated and contextual learning models such as problem-based learning (PBL) and brain/mind learning (BML) have become prominent. The present study aimed to develop and evaluate a PBL program enriched with BML principles. In this study, participants were 295 first-year medical students. The study used both quantitative and qualitative…

  16. Exploring the initial steps of the testing process: frequency and nature of pre-preanalytic errors.

    Science.gov (United States)

    Carraro, Paolo; Zago, Tatiana; Plebani, Mario

    2012-03-01

    Few data are available on the nature of errors in the so-called pre-preanalytic phase, the initial steps of the testing process. We therefore sought to evaluate pre-preanalytic errors using a study design that enabled us to observe the initial procedures performed in the ward, from the physician's test request to the delivery of specimens in the clinical laboratory. After a 1-week direct observational phase designed to identify the operating procedures followed in 3 clinical wards, we recorded all nonconformities and errors occurring over a 6-month period. Overall, the study considered 8547 test requests, for which 15 917 blood sample tubes were collected and 52 982 tests undertaken. No significant differences in error rates were found between the observational phase and the overall study period, but underfilling of coagulation tubes was found to occur more frequently in the direct observational phase (P = 0.043). In the overall study period, the frequency of errors was found to be particularly high regarding order transmission [29 916 parts per million (ppm)] and hemolysed samples (2537 ppm). The frequency of patient misidentification was 352 ppm, and the most frequent nonconformities were test requests recorded in the diary without the patient's name and failure to check the patient's identity at the time of blood draw. The data collected in our study confirm the relative frequency of pre-preanalytic errors and underline the need to consensually prepare and adopt effective standard operating procedures in the initial steps of laboratory testing and to monitor compliance with these procedures over time.

  17. The effect of centrifugation speed and time on pre-analytical platelet activation.

    Science.gov (United States)

    Söderström, Anna C; Nybo, Mads; Nielsen, Christian; Vinholt, Pernille J

    2016-12-01

    The results of laboratory analyses are affected by pre-analytical variables, and in particular can platelets be activated by shear handling stress and secrete granular substances. We therefore evaluated the effect of centrifugation speed and time on pre-analytical platelet activation. Citrate- and EDTA-anticoagulated blood from healthy volunteers were centrifuged at 80-10,000 g for 5-15 min to prepare plasma and platelet-rich plasma. Pre-analytical platelet activation was assessed by flow cytometric measurement of platelet P-selectin (CD62p) expression. Blood cell counts, mean platelet volume (MPV), immature platelet fraction (IPF), and platelet distribution width (PDW) were measured. Platelet aggregation in platelet-rich plasma induced by arachidonic acid (AA), ADP or thrombin receptor activator peptide-6 (TRAP) was tested by 96-well aggregometry. The median percentage of platelets expressing P-selectin in citrate- and EDTA-plasma centrifuged at 2000 g for 10 min were 43% [interquartile range (IQR), 38%-53%] and 56% (IQR, 31%-78%), respectively (p=0.82). Platelet-rich plasma prepared at 100-250 g for 10 min had significantly lower platelet P-selectin expression (11%-15%), pcentrifuged. In platelet-rich plasma, increasing centrifugation speed significantly increased platelet yield but decreased contamination from other blood cells, platelet composition was altered as platelet parameters (MPV, IPF, and PDW) was lowered. Platelet aggregation was not affected by the centrifugation speed platelet-rich plasma was prepared. Proportional to centrifugation speed, platelets in plasma and platelet-rich plasma were activated with centrifugation speed, cell content and composition changed while platelet aggregation was unaltered.

  18. Identification of clinical biomarkers for pre-analytical quality control of blood samples.

    Science.gov (United States)

    Kang, Hyun Ju; Jeon, Soon Young; Park, Jae-Sun; Yun, Ji Young; Kil, Han Na; Hong, Won Kyung; Lee, Mee-Hee; Kim, Jun-Woo; Jeon, Jae-Pil; Han, Bok Ghee

    2013-04-01

    Pre-analytical conditions are key factors in maintaining the high quality of biospecimens. They are necessary for accurate reproducibility of experiments in the field of biomarker discovery as well as achieving optimal specificity of laboratory tests for clinical diagnosis. In research at the National Biobank of Korea, we evaluated the impact of pre-analytical conditions on the stability of biobanked blood samples by measuring biochemical analytes commonly used in clinical laboratory tests. We measured 10 routine laboratory analytes in serum and plasma samples from healthy donors (n = 50) with a chemistry autoanalyzer (Hitachi 7600-110). The analyte measurements were made at different time courses based on delay of blood fractionation, freezing delay of fractionated serum and plasma samples, and at different cycles (0, 1, 3, 6, 9) of freeze-thawing. Statistically significant changes from the reference sample mean were determined using the repeated-measures ANOVA and the significant change limit (SCL). The serum levels of GGT and LDH were changed significantly depending on both the time interval between blood collection and fractionation and the time interval between fractionation and freezing of serum and plasma samples. The glucose level was most sensitive only to the elapsed time between blood collection and centrifugation for blood fractionation. Based on these findings, a simple formula (glucose decrease by 1.387 mg/dL per hour) was derived to estimate the length of time delay after blood collection. In addition, AST, BUN, GGT, and LDH showed sensitive responses to repeated freeze-thaw cycles of serum and plasma samples. These results suggest that GGT and LDH measurements can be used as quality control markers for certain pre-analytical conditions (eg, delayed processing or repeated freeze-thawing) of blood samples which are either directly used in the laboratory tests or stored for future research in the biobank.

  19. Effect of pre-analytical handling on haematological variables in minipigs

    DEFF Research Database (Denmark)

    Olsen, A K; Bladbjerg, E-M; Jensen, A L

    2001-01-01

    Pre-analytical handling may be an important determinant of haematological variables, if analysis is delayed. We investigated the effect of anticoagulants, i.e. tripotassium ethylenediamine-tetraacetic acid (EDTA) and citric acid, theophylline, adenosine, dipyridamole (CTAD), storage time (0.5, 1......, the samples must be stored in a refrigerator until analysis. Our studies underline that time delay before analysis of haematological variables can cause increased variation, and should therefore be limited as far as possible in order to reduce the number of animals needed to make reliable conclusions...

  20. Influence of common preanalytical variations on the metabolic profile of serum samples in biobanks

    International Nuclear Information System (INIS)

    Fliniaux, Ophélie; Gaillard, Gwenaelle; Lion, Antoine; Cailleu, Dominique; Mesnard, François; Betsou, Fotini

    2011-01-01

    A blood pre-centrifugation delay of 24 h at room temperature influenced the proton NMR spectroscopic profiles of human serum. A blood pre-centrifugation delay of 24 h at 4°C did not influence the spectroscopic profile as compared with 4 h delays at either room temperature or 4°C. Five or ten serum freeze–thaw cycles also influenced the proton NMR spectroscopic profiles. Certain common in vitro preanalytical variations occurring in biobanks may impact the metabolic profile of human serum.

  1. Influence of common preanalytical variations on the metabolic profile of serum samples in biobanks

    Energy Technology Data Exchange (ETDEWEB)

    Fliniaux, Ophelie [University of Picardie Jules Verne, Laboratoire de Phytotechnologie EA 3900-BioPI (France); Gaillard, Gwenaelle [Biobanque de Picardie (France); Lion, Antoine [University of Picardie Jules Verne, Laboratoire de Phytotechnologie EA 3900-BioPI (France); Cailleu, Dominique [Batiment Serres-Transfert, rue de Mai/rue Dallery, Plateforme Analytique (France); Mesnard, Francois, E-mail: francois.mesnard@u-picardie.fr [University of Picardie Jules Verne, Laboratoire de Phytotechnologie EA 3900-BioPI (France); Betsou, Fotini [Integrated Biobank of Luxembourg (Luxembourg)

    2011-12-15

    A blood pre-centrifugation delay of 24 h at room temperature influenced the proton NMR spectroscopic profiles of human serum. A blood pre-centrifugation delay of 24 h at 4 Degree-Sign C did not influence the spectroscopic profile as compared with 4 h delays at either room temperature or 4 Degree-Sign C. Five or ten serum freeze-thaw cycles also influenced the proton NMR spectroscopic profiles. Certain common in vitro preanalytical variations occurring in biobanks may impact the metabolic profile of human serum.

  2. Recommendations to standardize preanalytical confounding factors in Alzheimer's and Parkinson's disease cerebrospinal fluid biomarkers

    DEFF Research Database (Denmark)

    del Campo, Marta; Mollenhauer, Brit; Bertolotto, Antonio

    2012-01-01

    Early diagnosis of neurodegenerative disorders such as Alzheimer's (AD) or Parkinson's disease (PD) is needed to slow down or halt the disease at the earliest stage. Cerebrospinal fluid (CSF) biomarkers can be a good tool for early diagnosis. However, their use in clinical practice is challenging...... the need to establish standardized operating procedures. Here, we merge two previous consensus guidelines for preanalytical confounding factors in order to achieve one exhaustive guideline updated with new evidence for Aβ42, total tau and phosphorylated tau, and α-synuclein. The proposed standardized...

  3. Multimedia Design Principles in the Psychomotor Domain: The Effect of Multimedia and Spatial Contiguity on Students' Learning of Basic Life Support with Task Cards

    Science.gov (United States)

    Iserbyt, Peter; Mols, Liesbet; Elen, Jan; Behets, Daniel

    2012-01-01

    This study adds to the literature by introducing multimedia research in the psychomotor area. In this study, 87 freshman students in pedagogy used task cards to learn Basic Life Support (BLS), a psychomotor skill consisting of nine lifesaving actions to be performed in a specific order. Task cards are printed materials and are often implemented…

  4. Development of an e-learning system for teaching endoscopists how to diagnose early gastric cancer: basic principles for improving early detection.

    Science.gov (United States)

    Yao, Kenshi; Uedo, Noriya; Muto, Manabu; Ishikawa, Hideki

    2017-03-01

    We developed an internet e-learning system in order to improve the ability of endoscopists to diagnose gastric cancer at an early stage. The efficacy of this system at expanding knowledge and providing invaluable experience regarding the endoscopic detection of early gastric cancer was demonstrated through an international multicenter randomized controlled trial. However, the contents of the system have not yet been fully described in the literature. Accordingly, we herein introduce the contents and their principles, which comprise three main subjects: technique, knowledge, and experience. Since all the e-learning contents and principles are based on conventional white-light endoscopy alone, which is commonly available throughout the world, they should provide a good reference point for any endoscopist who wishes to devise learning materials and guidelines for improving their own clinical practice.

  5. Pre-analytical method for NMR-based grape metabolic fingerprinting and chemometrics.

    Science.gov (United States)

    Ali, Kashif; Maltese, Federica; Fortes, Ana Margarida; Pais, Maria Salomé; Verpoorte, Robert; Choi, Young Hae

    2011-10-10

    Although metabolomics aims at profiling all the metabolites in organisms, data quality is quite dependent on the pre-analytical methods employed. In order to evaluate current methods, different pre-analytical methods were compared and used for the metabolic profiling of grapevine as a model plant. Five grape cultivars from Portugal in combination with chemometrics were analyzed in this study. A common extraction method with deuterated water and methanol was found effective in the case of amino acids, organic acids, and sugars. For secondary metabolites like phenolics, solid phase extraction with C-18 cartridges showed good results. Principal component analysis, in combination with NMR spectroscopy, was applied and showed clear distinction among the cultivars. Primary metabolites such as choline, sucrose, and leucine were found discriminating for 'Alvarinho', while elevated levels of alanine, valine, and acetate were found in 'Arinto' (white varieties). Among the red cultivars, higher signals for citrate and GABA in 'Touriga Nacional', succinate and fumarate in 'Aragonês', and malate, ascorbate, fructose and glucose in 'Trincadeira', were observed. Based on the phenolic profile, 'Arinto' was found with higher levels of phenolics as compared to 'Alvarinho'. 'Trincadeira' showed lowest phenolics content while higher levels of flavonoids and phenylpropanoids were found in 'Aragonês' and 'Touriga Nacional', respectively. It is shown that the metabolite composition of the extract is highly affected by the extraction procedure and this consideration has to be taken in account for metabolomics studies. Copyright © 2011 Elsevier B.V. All rights reserved.

  6. Pre-analytical and analytical factors influencing Alzheimer's disease cerebrospinal fluid biomarker variability.

    Science.gov (United States)

    Fourier, Anthony; Portelius, Erik; Zetterberg, Henrik; Blennow, Kaj; Quadrio, Isabelle; Perret-Liaudet, Armand

    2015-09-20

    A panel of cerebrospinal fluid (CSF) biomarkers including total Tau (t-Tau), phosphorylated Tau protein at residue 181 (p-Tau) and β-amyloid peptides (Aβ42 and Aβ40), is frequently used as an aid in Alzheimer's disease (AD) diagnosis for young patients with cognitive impairment, for predicting prodromal AD in mild cognitive impairment (MCI) subjects, for AD discrimination in atypical clinical phenotypes and for inclusion/exclusion and stratification of patients in clinical trials. Due to variability in absolute levels between laboratories, there is no consensus on medical cut-off value for the CSF AD signature. Thus, for full implementation of this core AD biomarker panel in clinical routine, this issue has to be solved. Variability can be explained both by pre-analytical and analytical factors. For example, the plastic tubes used for CSF collection and storage, the lack of reference material and the variability of the analytical protocols were identified as important sources of variability. The aim of this review is to highlight these pre-analytical and analytical factors and describe efforts done to counteract them in order to establish cut-off values for core CSF AD biomarkers. This review will give the current state of recommendations. Copyright © 2015. Published by Elsevier B.V.

  7. Basic physics for all

    CERN Document Server

    Kumar, B N

    2012-01-01

    This is a simple, concise book for both student and non-physics students, presenting basic facts in straightforward form and conveying fundamental principles and theories of physics. This book will be helpful as a supplement to class teaching and to aid those who have difficulty in mastering concepts and principles.

  8. The preanalytical optimization of blood cultures: a review and the clinical importance of benchmarking in 5 Belgian hospitals.

    Science.gov (United States)

    Willems, Elise; Smismans, Annick; Cartuyvels, Reinoud; Coppens, Guy; Van Vaerenbergh, Kristien; Van den Abeele, Anne-Marie; Frans, Johan

    2012-05-01

    Bloodstream infections remain a major challenge in medicine. Optimal detection of pathogens is only possible if the quality of preanalytical factors is thoroughly controlled. Since the laboratory is responsible for this preanalytical phase, the quality control of critical factors should be integrated in its quality control program. The numerous recommendations regarding blood culture collection contain controversies. Only an unambiguous guideline permits standardization and interlaboratory quality control. We present an evidence-based concise guideline of critical preanalytical determinants for blood culture collection and summarize key performance indicators with their concomitant target values. In an attempt to benchmark, we compared the true-positive rate, contamination rate, and collected blood volume of blood culture bottles in 5 Belgian hospital laboratories. The true-positive blood culture rate fell within previously defined acceptation criteria by Baron et al. (2005) in all 5 hospitals, whereas the contamination rate exceeded the target value in 4 locations. Most unexpected, in each of the 5 laboratories, more than one third of the blood culture bottles were incorrectly filled, irrespective of the manufacturer of the blood culture vials. As a consequence of this shortcoming, one manufacturer recently developed an automatic blood volume monitoring system. In conclusion, clear recommendations for standardized blood culture collection combined with quality control of critical factors of the preanalytical phase are essential for diagnostic blood culture improvement. Copyright © 2012 Elsevier Inc. All rights reserved.

  9. Pre-analytical variables of circulating cell-free nucleosomes containing 5-methylcytosine DNA or histone modification H3K9Me3

    DEFF Research Database (Denmark)

    Rasmussen, Louise; Herzog, Marielle; Rømer, Eva

    2016-01-01

    Aim: To evaluate pre-analytical variables of circulating cell-free nucleosomes containing 5-methylcytosine DNA (5mC) or histone modification H3K9Me3 (H3K9Me3). Materials and methods: Six studies were designed to assess the possible influence of pre-analytical variables. Study 1: influence of stasis...... significantly lower levels of 5mC or H3K9Me3 compared to levels in healthy individuals. Conclusion: Levels of 5mC or H3K9Me3 appear stable in most pre-analytical settings if blood samples are stored at room temperature until centrifugation....

  10. Basic digital signal processing

    CERN Document Server

    Lockhart, Gordon B

    1985-01-01

    Basic Digital Signal Processing describes the principles of digital signal processing and experiments with BASIC programs involving the fast Fourier theorem (FFT). The book reviews the fundamentals of the BASIC program, continuous and discrete time signals including analog signals, Fourier analysis, discrete Fourier transform, signal energy, power. The text also explains digital signal processing involving digital filters, linear time-variant systems, discrete time unit impulse, discrete-time convolution, and the alternative structure for second order infinite impulse response (IIR) sections.

  11. Zymography Principles.

    Science.gov (United States)

    Wilkesman, Jeff; Kurz, Liliana

    2017-01-01

    Zymography, the detection, identification, and even quantification of enzyme activity fractionated by gel electrophoresis, has received increasing attention in the last years, as revealed by the number of articles published. A number of enzymes are routinely detected by zymography, especially with clinical interest. This introductory chapter reviews the major principles behind zymography. New advances of this method are basically focused towards two-dimensional zymography and transfer zymography as will be explained in the rest of the chapters. Some general considerations when performing the experiments are outlined as well as the major troubleshooting and safety issues necessary for correct development of the electrophoresis.

  12. 5. The Basic Principles of The Working Process From Nature and the Role of Composition in Creating Landscape in Terms of Plein-Air

    Directory of Open Access Journals (Sweden)

    Savițkaia-Baraghin Iarîna

    2017-03-01

    Full Text Available Principles of possession of the landscape painting, applied by masters of the past, as well as theoretical elaborations of scientists in the field of chromatics, psychology and pedagogy of arts, become important components in improving training methods of artists in plein-air in contemporary conditions. The skills and knowledge gained in the process of pleinair studies form professional skills and improve the properties of painting and composition within the workshop. This studying outlines the stringency of training and development methods of creative individuality, especially in plein-air, where the principal teacher is the nature. The plein-air enriches color perception of the real world, located in the air, and mutual relationship of landscape with architecture and space determines knowledge of the issues of proportionality and subordination in compositions, educates sense of proportion and artistic taste. Improving visual mastery into the natural environment contributes to the formation to painters of the necessity to create outside the workshop, which is a necessary condition for the development of individuality and professional skills of the painter.

  13. El muestreo en investigación cualitativa: principios básicos y algunas controversias Sampling in qualitative research: basic principles and some controversies

    Directory of Open Access Journals (Sweden)

    Carolina Martínez-Salgado

    2012-03-01

    Full Text Available En este trabajo se presentan los fundamentos de la elección de los participantes en una investigación cualitativa, en contraste con los que rigen al muestreo probabilístico en la investigación epidemiológica. Se proponen los conceptos de generalizabilidad nomotética e ideográfica, y los de transferibilidad y reflexividad, para una mejor comprensión de las diferencias. Se mencionan los fundamentos de los principales tipos de muestreo que suelen utilizarse en investigación cualitativa, el significado del concepto de saturación y algunos de sus cuestionamientos. Por último, se plantean algunas reflexiones en torno a las controversias suscitadas en los últimos años sobre las diversas perspectivas paradigmáticas desde las cuales se puede efectuar hoy día la investigación cualitativa, sus posibilidades de combinación con la investigación epidemiológica, y algunas implicaciones para el estudio de los problemas de salud.This paper presents the rationale for the choice of participants in qualitative research in contrast with that of probability sampling principles in epidemiological research. For a better understanding of the differences, concepts of nomothetic and ideographic generalizability, as well as those of transferability and reflexivity, are proposed, Fundamentals of the main types of sampling commonly used in qualitative research, and the meaning of the concept of saturation are mentioned. Finally, some reflections on the controversies that have arisen in recent years on various paradigmatic perspectives from which to conduct qualitative research, their possibilities of combination with epidemiological research, and some implications for the study of health issues are presented.

  14. Vaccinology: principles and practice

    National Research Council Canada - National Science Library

    Morrow, John

    2012-01-01

    ... principles to implementation. This is an authoritative textbook that details a comprehensive and systematic approach to the science of vaccinology focusing on not only basic science, but the many stages required to commercialize...

  15. Biomarker Analysis of Stored Blood Products: Emphasis on Pre-Analytical Issues

    Science.gov (United States)

    Delobel, Julien; Rubin, Olivier; Prudent, Michel; Crettaz, David; Tissot, Jean-Daniel; Lion, Niels

    2010-01-01

    Millions of blood products are transfused every year; many lives are thus directly concerned by transfusion. The three main labile blood products used in transfusion are erythrocyte concentrates, platelet concentrates and fresh frozen plasma. Each of these products has to be stored according to its particular components. However, during storage, modifications or degradation of those components may occur, and are known as storage lesions. Thus, biomarker discovery of in vivo blood aging as well as in vitro labile blood products storage lesions is of high interest for the transfusion medicine community. Pre-analytical issues are of major importance in analyzing the various blood products during storage conditions as well as according to various protocols that are currently used in blood banks for their preparations. This paper will review key elements that have to be taken into account in the context of proteomic-based biomarker discovery applied to blood banking. PMID:21151459

  16. [Pre-analytical stability before centrifugation of 7 biochemical analytes in whole blood].

    Science.gov (United States)

    Perrier-Cornet, Andreas; Moineau, Marie-Pierre; Narbonne, Valérie; Plee-Gautier, Emmanuelle; Le Saos, Fabienne; Carre, Jean-Luc

    2015-01-01

    The pre-analytical stability of 7 biochemical parameters (parathyroid hormone -PTH-, vitamins A, C E and D, 1,25-dihydroxyvitamin D and insulin) at +4 °C, was studied on whole blood samples before centrifugation. The impact of freezing at -20°C was also analyzed/performed for PTH and vitamin D. The differences in the results of assays for whole blood samples, being kept for different times between sampling time and analysis, from 9 healthy adults, were compaired by using a Student t test. The 7 analytes investigated remained stable up to 4 hours at +4°C in whole blood. This study showed that it is possible to accept uncentrifuged whole blood specimens kept at +4°C before analysis. PTH is affected by freezing whereas vitamin D is not.

  17. Effects of fecal sampling on preanalytical and analytical phases in quantitative fecal immunochemical tests for hemoglobin.

    Science.gov (United States)

    Rapi, Stefano; Berardi, Margherita; Cellai, Filippo; Ciattini, Samuele; Chelazzi, Laura; Ognibene, Agostino; Rubeca, Tiziana

    2017-07-24

    Information on preanalytical variability is mandatory to bring laboratories up to ISO 15189 requirements. Fecal sampling is greatly affected by lack of harmonization in laboratory medicine. The aims of this study were to obtain information on the devices used for fecal sampling and to explore the effect of different amounts of feces on the results from the fecal immunochemical test for hemoglobin (FIT-Hb). Four commercial sample collection devices for quantitative FIT-Hb measurements were investigated. The volume of interest (VOI) of the probes was measured from diameter and geometry. Quantitative measurements of the mass of feces were carried out by gravimetry. The effects of an increased amount of feces on the analytical environment were investigated measuring the Hb values with a single analytical method. VOI was 8.22, 7.1 and 9.44 mm3 for probes that collected a target of 10 mg of feces, and 3.08 mm3 for one probe that targeted 2 mg of feces. The ratio between recovered and target amounts of devices ranged from 56% to 121%. Different changes in the measured Hb values were observed, in adding increasing amounts of feces in commercial buffers. The amounts of collected materials are related to the design of probes. Three out 4 manufacturers declare the same target amount using different sampling volumes and obtaining different amounts of collected materials. The introduction of a standard probes to reduce preanalytical variability could be an useful step for fecal test harmonization and to fulfill the ISO 15189 requirements.

  18. Recommendations from Gynaecological (GYN) GEC-ESTRO Working Group (IV): Basic principles and parameters for MR imaging within the frame of image based adaptive cervix cancer brachytherapy

    International Nuclear Information System (INIS)

    Dimopoulos, Johannes C.A.; Petrow, Peter; Tanderup, Kari; Petric, Primoz; Berger, Daniel; Kirisits, Christian; Pedersen, Erik M.; Limbergen, Erik van; Haie-Meder, Christine; Pötter, Richard

    2012-01-01

    The GYN GEC-ESTRO working group issued three parts of recommendations and highlighted the pivotal role of MRI for the successful implementation of 3D image-based cervical cancer brachytherapy (BT). The main advantage of MRI as an imaging modality is its superior soft tissue depiction quality. To exploit the full potential of MRI for the better ability of the radiation oncologist to make the appropriate choice for the BT application technique and to accurately define the target volumes and the organs at risk, certain MR imaging criteria have to be fulfilled. Technical requirements, patient preparation, as well as image acquisition protocols have to be tailored to the needs of 3D image-based BT. The present recommendation is focused on the general principles of MR imaging for 3D image-based BT. Methods and parameters have been developed and progressively validated from clinical experience from different institutions (IGR, Universities of Vienna, Leuven, Aarhus and Ljubljana) and successfully applied during expert meetings, contouring workshops, as well as within clinical and interobserver studies. It is useful to perform pelvic MRI scanning prior to radiotherapy (“Pre-RT-MRI examination”) and at the time of BT (“BT MRI examination”) with one MR imager. Both low and high-field imagers, as well as both open and close magnet configurations conform to the requirements of 3D image-based cervical cancer BT. Multiplanar (transversal, sagittal, coronal and oblique image orientation) T2-weighted images obtained with pelvic surface coils are considered as the golden standard for visualisation of the tumour and the critical organs. The use of complementary MRI sequences (e.g. contrast-enhanced T1-weighted or 3D isotropic MRI sequences) is optional. Patient preparation has to be adapted to the needs of BT intervention and MR imaging. It is recommended to visualise and interpret the MR images on dedicated DICOM-viewer workstations, which should also assist the contouring

  19. Microwave system engineering principles

    CERN Document Server

    Raff, Samuel J

    1977-01-01

    Microwave System Engineering Principles focuses on the calculus, differential equations, and transforms of microwave systems. This book discusses the basic nature and principles that can be derived from thermal noise; statistical concepts and binomial distribution; incoherent signal processing; basic properties of antennas; and beam widths and useful approximations. The fundamentals of propagation; LaPlace's Equation and Transmission Line (TEM) waves; interfaces between homogeneous media; modulation, bandwidth, and noise; and communications satellites are also deliberated in this text. This bo

  20. The basic principles for the assessment of occupational exposure due to intakes of radionuclides (The most important indirect methods used in the Protection and Safety Dept. for internal occupational exposure monitoring)

    International Nuclear Information System (INIS)

    Kharita, M. H.; Sakhita, K.

    2009-05-01

    This study shows the basic principles for the assessment of occupational exposure due to intakes of radionuclides in order to calculate the committed effective dose of each radionuclide separately. We also discussed when the routine monitoring of workers becomes useful and when the workplace monitoring is better than workers monitoring. In addition, this study contains the details of four indirect methods as they are validated in the protection and safety department, and their names are: Determination the concentration of total uranium by using fluorimetry technique, Determination the activity of two uranium isotopes 238 and 234, The activity of Polonium 210, and the activity of Radium 226 by using alpha spectrometry for urine samples collected from workers occupationally exposed to this isotope. (author)

  1. Risk communication basics

    International Nuclear Information System (INIS)

    Corrado, P.G.

    1995-01-01

    In low-trust, high-concern situations, 50% of your credibility comes from perceived empathy and caring, demonstrated in the first 30 s you come in contact with someone. There is no second chance for a first impression. These and other principles contained in this paper provide you with a basic level of understanding of risk communication. The principles identified are time-tested caveats and will assist you in effectively communicating technical information

  2. Risk communication basics

    Energy Technology Data Exchange (ETDEWEB)

    Corrado, P.G. [Lawrence Livermore National Laboratory, CA (United States)

    1995-12-31

    In low-trust, high-concern situations, 50% of your credibility comes from perceived empathy and caring, demonstrated in the first 30 s you come in contact with someone. There is no second chance for a first impression. These and other principles contained in this paper provide you with a basic level of understanding of risk communication. The principles identified are time-tested caveats and will assist you in effectively communicating technical information.

  3. Pre-analytical and biological variability in circulating interleukin 6 in healthy subjects and patients with rheumatoid arthritis

    DEFF Research Database (Denmark)

    Knudsen, Lene S; Christensen, Ib J; Lottenburger, Tine

    2008-01-01

    in serum IL-6 over a 3-year period (14%, p=0.33). Exercise (cycling) increased serum IL-6 in healthy subjects but not in RA patients. In conclusion, circulating IL-6 is stable regarding sample handling and shows little variation over time. Changes in IL-6 concentrations >60% (2 times the biological...... variation) are likely to reflect changes in disease activity and not only pre-analytical or normal biological variability....

  4. Preanalytical errors in primary healthcare: a questionnaire study of information search procedures, test request management and test tube labelling.

    Science.gov (United States)

    Söderberg, Johan; Brulin, Christine; Grankvist, Kjell; Wallin, Olof

    2009-01-01

    Most errors in laboratory medicine occur in the preanalytical phase and are the result of human mistakes. This study investigated information search procedures, test request management and test tube labelling in primary healthcare compared to the same procedures amongst clinical laboratory staff. A questionnaire was completed by 317 venous blood sampling staff in 70 primary healthcare centres and in two clinical laboratories (response rate = 94%). Correct procedures were not always followed. Only 60% of the primary healthcare staff reported that they always sought information in the updated, online laboratory manual. Only 12% reported that they always labelled the test tubes prior to drawing blood samples. No major differences between primary healthcare centres and clinical laboratories were found, except for test tube labelling, whereby the laboratory staff reported better practices. Re-education and access to documented routines were not clearly associated with better practices. The preanalytical procedure in the surveyed primary healthcare centres was associated with a risk of errors which could affect patient safety. To improve patient safety in laboratory testing, all healthcare providers should survey their preanalytical procedures and improve the total testing process with a systems perspective.

  5. Cosmological principles. II. Physical principles

    International Nuclear Information System (INIS)

    Harrison, E.R.

    1974-01-01

    The discussion of cosmological principle covers the uniformity principle of the laws of physics, the gravitation and cognizability principles, and the Dirac creation, chaos, and bootstrap principles. (U.S.)

  6. Pre-analytical and analytical variation of drug determination in segmented hair using ultra-performance liquid chromatography-tandem mass spectrometry.

    Science.gov (United States)

    Nielsen, Marie Katrine Klose; Johansen, Sys Stybe; Linnet, Kristian

    2014-01-01

    Assessment of total uncertainty of analytical methods for the measurements of drugs in human hair has mainly been derived from the analytical variation. However, in hair analysis several other sources of uncertainty will contribute to the total uncertainty. Particularly, in segmental hair analysis pre-analytical variations associated with the sampling and segmentation may be significant factors in the assessment of the total uncertainty budget. The aim of this study was to develop and validate a method for the analysis of 31 common drugs in hair using ultra-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) with focus on the assessment of both the analytical and pre-analytical sampling variations. The validated method was specific, accurate (80-120%), and precise (CV≤20%) across a wide linear concentration range from 0.025-25 ng/mg for most compounds. The analytical variation was estimated to be less than 15% for almost all compounds. The method was successfully applied to 25 segmented hair specimens from deceased drug addicts showing a broad pattern of poly-drug use. The pre-analytical sampling variation was estimated from the genuine duplicate measurements of two bundles of hair collected from each subject after subtraction of the analytical component. For the most frequently detected analytes, the pre-analytical variation was estimated to be 26-69%. Thus, the pre-analytical variation was 3-7 folds larger than the analytical variation (7-13%) and hence the dominant component in the total variation (29-70%). The present study demonstrated the importance of including the pre-analytical variation in the assessment of the total uncertainty budget and in the setting of the 95%-uncertainty interval (±2CVT). Excluding the pre-analytical sampling variation could significantly affect the interpretation of results from segmental hair analysis. Copyright © 2013 Elsevier Ireland Ltd. All rights reserved.

  7. Chasing the effects of Pre-analytical Confounders - a Multicentre Study on CSF-AD biomarkers

    Directory of Open Access Journals (Sweden)

    Maria Joao Leitao

    2015-07-01

    Full Text Available Core cerebrospinal fluid (CSF biomarkers-Aβ42, Tau and pTau–have been recently incorporated in the revised criteria for Alzheimer’s disease (AD. However, their widespread clinical application lacks standardization. Pre-analytical sample handling and storage play an important role in the reliable measurement of these biomarkers across laboratories. In this study, we aim to surpass the efforts from previous studies, by employing a multicentre approach to assess the impact of less studied CSF pre-analytical confounders in AD-biomarkers quantification. Four different centres participated in this study and followed the same established protocol. CSF samples were analysed for three biomarkers (Aβ42, Tau and pTau and tested for different spinning conditions (temperature: Room temperature (RT vs. 4oC; speed: 500g vs. 2000g vs. 3000g, storage volume variations (25%, 50% and 75% of tube total volume as well as freezing-thaw cycles (up to 5 cyles. The influence of sample routine parameters, inter-centre variability and relative value of each biomarker (reported as normal/abnormal, was analysed. Centrifugation conditions did not influence biomarkers levels, except for samples with a high CSF total protein content, where either non centrifugation or centrifugation at RT, compared to 4ºC, led to higher Aβ42 levels. Reducing CSF storage volume from 75% to 50% of total tube capacity, decreased Aβ42 concentration (within analytical CV of the assay, whereas no change in Tau or pTau was observed. Moreover, the concentration of Tau and pTau appears to be stable up to 5 freeze-thaw cycles, whereas Aβ42 levels decrease if CSF is freeze-thawed more than 3 times. This systematic study reinforces the need for CSF centrifugation at 4ºC prior to storage and highlights the influence of storage conditions in Aβ42 levels. This study contributes to the establishment of harmonized standard operating procedures that will help reducing inter-lab variability of CSF

  8. MIGRAINE: BASIC PRINCIPLES OF TREATMENT AND PREVENTION

    Directory of Open Access Journals (Sweden)

    O. R. Esin

    2011-01-01

    Full Text Available Modern recommendations for the migraine attack treatment and it's prophylaxis are analyzed in this review. Established, that acetylsalicylic acid, diclofenac potassium, ibuprofen, naproxen, paracetamol, metamizol and their combination with caffeine are drugs of the first choice for migraine attack treatment. Metoclopramide and domperidone are used to reduce nausea and vomiting. Also triptans are high effective drugs for migraine attack treatment. Metoprolol, propranolol, flunarizine, valproic acid can be used for migraine prophylaxis. Drugs of the second choice are: amitriptyline, venlafaxine, naproxen and bisoprolol.

  9. Basic Principles and Concepts for Achieving Quality

    National Research Council Canada - National Science Library

    Baker, Emanuel R; Fisher, Matthew J; Goethert, Wolfhart; Marino, Lisa

    2007-01-01

    ...) developed in the early 1980s for the Department of Defense (DoD) by Baker and colleagues. The original quality concepts of the SQF are extended beyond software to include products, services, and processes...

  10. X-ray Spectrometry: Basic principles

    International Nuclear Information System (INIS)

    Carvalho, R.M.; Teixeira, G.J.; Cardoso, R.S.; Peixoto, J.G.P.

    2017-01-01

    The application of X rays requires a study of its spectrum. Intrinsic difficulties of the own method and of all the instrumentation necessary for the accomplishment of this practice were related. The objective was to demonstrate the use of a commercial spectrometer using at room temperature and compare it with spectra theoretically obtained by simulation. As an initial result was that both instrumentation is compatible to be used in an X-ray beam, with and without scattering material and its theoretical data were obtained. (author)

  11. Nutrition in pregnancy: Basic principles and recommendations

    Directory of Open Access Journals (Sweden)

    Plećaš Draga

    2014-01-01

    Full Text Available Healthy diet in pregnancy should guarantee proper fetal growth and development, maintain (and promote maternal health and enable lactation. Nutritional counseling and interventions need to be an integral part of antenatal care and continue during pregnancy in order to reduce the risk of maternal, fetal and neonatal complications, as well as the short- and long-term adverse outcomes. Adverse pregnancy outcomes are more common in women who begin the gestation as undernourished or obese in comparison to pregnant women whose weight is within normal ranges. Increased nutritional and energy needs in pregnancy are met through numerous metabolic adaptations; pregnancy is successfully achieved within wide range of variations in energy supply and weight gain. However, if nutrient restriction exceeds the limits of adaptive responses, evidence indicates that fetus will develop the alternative metabolic competence that might emerge as a disease (type 2 diabetes, hypertension, coronary heart disease and stroke in adult life.

  12. Basic principles of drug--excipients interactions.

    Science.gov (United States)

    Vranić, Edina

    2004-05-01

    Excipients are generally considered inert additives included in drug formulation to help in the manufacturing, administration or absorption. Other reasons for inclusion concern product differentiation, appearance enhancement or retention of quality. Excipients can initiate, propagate or participate in chemical or physical interactions with an active substance, possibly leading to compromised quality or performance of the medication. Understanding the chemical and physical nature of excipients, the impurities or residues associated with them and how they may interact with other materials, or with each other, forewarns the pharmaceutical technologist of possibilities for undesirable developments.

  13. Basic principles of Swiss energy policy

    International Nuclear Information System (INIS)

    Kiener, E.

    1979-01-01

    The author shows that Swiss energy problems, and the measures to be adopted for their solution, are similar to those in other industrial countries. For Switzerland water power is still the most important indigenous energy source. In energy policy it is necessary to make economies. It is important that those responsible for energy policy tackle the problems today, and not leave it to a time when it is too late. The author is convinced that science and engineering will make much more progress in the energy field. (orig.) [de

  14. Basic principles for chronic peritoneal dialysis prescription

    Directory of Open Access Journals (Sweden)

    Roberto J. Barone

    2016-09-01

    Full Text Available La diálisis peritoneal, como tratamiento sustitutivo de la función renal, se basa en la instilación dentro de la cavidad peritoneal de soluciones de diálisis con el objeto de aprovechar algunas de las propiedades del peritoneo como membrana biológica, con el fin de eliminar las sustancias de desecho generadas diariamente por nuestro organismo y contribuir entre otros con el control del balance hidrosalino alterado en la insuficiencia renal.

  15. Software Defined Radio: Basic Principles and Applications

    Directory of Open Access Journals (Sweden)

    José Raúl Machado-Fernández

    2014-12-01

    Full Text Available The author makes a review of the SDR (Software Defined Radio technology, including hardware schemes and application fields. A low performance device is presented and several tests are executed with it using free software. With the acquired experience, SDR employment opportunities are identified for low-cost solutions that can solve significant problems. In addition, a list of the most important frameworks related to the technology developed in the last years is offered, recommending the use of three of them.

  16. Basic principles of ultrafast Raman loss spectroscopy

    Indian Academy of Sciences (India)

    field tries to drive the electron with its frequency. If the applied field is of the form E = E0eiωt , the driving ..... (CARS),8 coherent Stokes Raman scattering (CSRS), ... gain or loss process in which only two beams are required and it is a self ...

  17. Laser light scattering basic principles and practice

    CERN Document Server

    Chu, Benjamin

    1994-01-01

    Geared toward upper-level undergraduate and graduate students, this text introduces the interdisciplinary area of laser light scattering, focusing chiefly on theoretical concepts of quasielastic laser scattering.

  18. BASIC PRINCIPLES OF PUPIL’S DEVELOPMENT

    Directory of Open Access Journals (Sweden)

    Sabina Mujčin

    2009-11-01

    Full Text Available “Physical education is consciousness activity, constant from childhood to adult- hood, to which the aim is to assure full physical development, to strengthen the power of resistance against illness, to ameliorate energy and all other characteristics of ac- tivity and character.” (Hebert / Le sport contre l’éducation physique“, Pons 1925.. Physycal education is important factor of overall education. It becomes equal and equally valuable process, which features special tools and tasks. However, besides everything else success of physical education of children and youth depends on methods, content, tools, sources and school organization of teaching. The science has universal deve- lopment of the personality as the main goal. Following that every education which’s results The science puts overall development of personality as goal of education. Follo- wing that, every education that opposes to that goal is wrong and narrow. This means that physical education as composing and indivisible part of general education is permanent, planned and systematic process of impact on human, especially at the period of youth when different kinds of tools, especially by physical education develop and build a person capable for work, and when forming his personality in sense of ethics Yet, besides all other a success of physical education of children and youth depend on methods, content, tools, sources and school organization of teaching. For an achie- vement of expected results it is not enough that teacher knows only meanings, aims and connectivity of his work in function of school as educational institution, but also to know to organize and perform teaching by modern scientific achievement, specially pedagogy, psychology and physiology.

  19. BASIC PRINCIPLES OF PUPIL’S DEVELOPMENT

    OpenAIRE

    Sabina Mujčin

    2009-01-01

    “Physical education is consciousness activity, constant from childhood to adult- hood, to which the aim is to assure full physical development, to strengthen the power of resistance against illness, to ameliorate energy and all other characteristics of ac- tivity and character.” (Hebert / Le sport contre l’éducation physique“, Pons 1925.). Physycal education is important factor of overall education. It becomes equal and equally valuable process, which features special tools and tasks. However...

  20. Basic principles of cone beam computed tomography.

    Science.gov (United States)

    Abramovitch, Kenneth; Rice, Dwight D

    2014-07-01

    At the end of the millennium, cone-beam computed tomography (CBCT) heralded a new dental technology for the next century. Owing to the dramatic and positive impact of CBCT on implant dentistry and orthognathic/orthodontic patient care, additional applications for this technology soon evolved. New software programs were developed to improve the applicability of, and access to, CBCT for dental patients. Improved, rapid, and cost-effective computer technology, combined with the ability of software engineers to develop multiple dental imaging applications for CBCT with broad diagnostic capability, have played a large part in the rapid incorporation of CBCT technology into dentistry. Copyright © 2014 Elsevier Inc. All rights reserved.

  1. Basic principles for measurement of intramuscular pressure

    Science.gov (United States)

    Hargens, A. R.; Ballard, R. E.

    1995-01-01

    We review historical and methodological approaches to measurements of intramuscular pressure (IMP) in humans. These techniques provide valuable measures of muscle tone and activity as well as diagnostic criteria for evaluation of exertional compartment syndrome. Although the wick and catheter techniques provide accurate measurements of IMP at rest, their value for exercise studies and diagnosis of exertional compartment syndrome is limited because of low frequency response and hydrostatic (static and inertial) pressure artifacts. Presently, most information on diagnosis of exertional compartment syndromes during dynamic exercise is available using the Myopress catheter. However, future research and clinical diagnosis using IMP can be optimized by the use of a miniature transducer-tipped catheter such as the Millar Mikro-tip.

  2. Laser Treatment of Tattoos: Basic Principles.

    Science.gov (United States)

    Bäumler, Wolfgang

    2017-01-01

    Tattooing has become very popular worldwide during the past decades, and millions of people have one or many tattoos at different anatomical sites. The color of tattoos is mainly black, followed by red, green, blue, and other colors. A part of the tattooed people regret tattooing or have permanent problems with tattoos and therefore seek for tattoo removal. Tattoos consist of solid pigment particles in the skin. Thus, tattoo removal requires fragmentation of these permanently incorporated particles. The gold standard of tattoo removal is laser therapy. Short light pulses at high intensities are applied to the tattooed skin surface. The laser light penetrates the skin and is selectively absorbed in the pigment particles. The absorbed laser light leads to heat-up and fragmentation of the particles. Due to the complex chemistry of the various tattoo pigments, the efficacy of this fragmentation process is frequently unpredictable. Due to the short and intense pulses, nonlinear effects of light and thermal properties of tattoo particles may play a role, and the assumptions of selective photothermolysis may not reflect the real process of tattoo particle fragmentation as a whole. In case fragmentation occurs, the concentration of pigment particles in the skin decreases, yielding a fading of the tattoo color in the skin. Laser therapy is most effective in black tattoos and less effective for colored tattoos. The rate of side effects is low due to the selectivity of the treatment. Laser light may change the chemistry of the tattoo pigments and hence provoke toxic decomposition products. © 2017 S. Karger AG, Basel.

  3. The woollen system- development and basic principles

    CSIR Research Space (South Africa)

    Van der Merwe, JP

    1984-03-01

    Full Text Available .................. ..................... 4.9 Fibre transfer between swift and doffer ..................................... 4.10 WooUencondensing ............................ 4.10.1 Ring doffer condensers 4.10.2 Tape condensers.... For the dusting or shaking process a double-cylinder opening machine is often used, as shown in Figure 2. Sand, dirt and dust are removed by the rotating spiked beaters which heat the wool against the screens at the bottom of the machine. Fig. 2 - Doubk...

  4. Industrial metabolism : roots and basic principles

    NARCIS (Netherlands)

    Lambert, A.J.D.; Gupta, S.M.; Lambert, A.J.D.

    2008-01-01

    Terms such as "life cyele management," "industrial ecology," "industrial metabolism," and "industrial symbiosis" are common in the literature on enviwnmentally conscious production, but the precise definition of these concepts and the distinction between them varies with authors. For clarity on

  5. Digital design basic concepts and principles

    CERN Document Server

    Karim, Mohammad A

    2007-01-01

    DATA TYPE AND REPRESENTATIONS Positional Number Systems Number System Conversion Negative Numbers Binary Arithmetic Unconventional Number System Binary Codes Error Detecting and Correcting Codes CAD System BOOLEAN ALGEBRA Logic Operations Logic Functions from Truth Tables Boolean Algebra MINIMIZATION OF LOGIC FUNCTIONS Karnaugh Map Incompletely Specified Functions in K-Map K-Maps for Product-of-sum Form of Functions Map-entered Variables Hazards Single-output Q-M Tabular Reduction Multiple-output Q-M Tabular reduction                               LOGIC FUNCTION IMPLEMENTATION Introduction Functionally Complete Operation Sets NAND-only and NOR-only Implementations Function Implementation Using XOR and XNOR Logic Circuit Implementation Using Gate Arrays Logic Function Implementation Using Multiplexers Logic Function Implementation Using Demultiplexers andecoders Logic Function Implementation Using ROM Logic Function Implementation Using PLD Logic Func...

  6. Quantum electronics basic theory

    CERN Document Server

    Fain, V M; Sanders, J H

    1969-01-01

    Quantum Electronics, Volume 1: Basic Theory is a condensed and generalized description of the many research and rapid progress done on the subject. It is translated from the Russian language. The volume describes the basic theory of quantum electronics, and shows how the concepts and equations followed in quantum electronics arise from the basic principles of theoretical physics. The book then briefly discusses the interaction of an electromagnetic field with matter. The text also covers the quantum theory of relaxation process when a quantum system approaches an equilibrium state, and explai

  7. Highly Sensitive and Selective Uranium Detection in Natural Water Systems Using a Luminescent Mesoporous Metal-Organic Framework Equipped with Abundant Lewis Basic Sites: A Combined Batch, X-ray Absorption Spectroscopy, and First Principles Simulation Investigation.

    Science.gov (United States)

    Liu, Wei; Dai, Xing; Bai, Zhuanling; Wang, Yanlong; Yang, Zaixing; Zhang, Linjuan; Xu, Lin; Chen, Lanhua; Li, Yuxiang; Gui, Daxiang; Diwu, Juan; Wang, Jianqiang; Zhou, Ruhong; Chai, Zhifang; Wang, Shuao

    2017-04-04

    Uranium is not only a strategic resource for the nuclear industry but also a global contaminant with high toxicity. Although several strategies have been established for detecting uranyl ions in water, searching for new uranium sensor material with great sensitivity, selectivity, and stability remains a challenge. We introduce here a hydrolytically stable mesoporous terbium(III)-based MOF material compound 1, whose channels are as large as 27 Å × 23 Å and are equipped with abundant exposed Lewis basic sites, the luminescence intensity of which can be efficiently and selectively quenched by uranyl ions. The detection limit in deionized water reaches 0.9 μg/L, far below the maximum contamination standard of 30 μg/L in drinking water defined by the United States Environmental Protection Agency, making compound 1 currently the only MOF material that can achieve this goal. More importantly, this material exhibits great capability in detecting uranyl ions in natural water systems such as lake water and seawater with pH being adjusted to 4, where huge excesses of competing ions are present. The uranyl detection limits in Dushu Lake water and in seawater were calculated to be 14.0 and 3.5 μg/L, respectively. This great detection capability originates from the selective binding of uranyl ions onto the Lewis basic sites of the MOF material, as demonstrated by synchrotron radiation extended X-ray adsorption fine structure, X-ray adsorption near edge structure, and first principles calculations, further leading to an effective energy transfer between the uranyl ions and the MOF skeleton.

  8. Fermat and the Minimum Principle

    Indian Academy of Sciences (India)

    Arguably, least action and minimum principles were offered or applied much earlier. This (or these) principle(s) is/are among the fundamental, basic, unifying or organizing ones used to describe a variety of natural phenomena. It considers the amount of energy expended in performing a given action to be the least required ...

  9. Fundamental Principle for Quantum Theory

    OpenAIRE

    Khrennikov, Andrei

    2002-01-01

    We propose the principle, the law of statistical balance for basic physical observables, which specifies quantum statistical theory among all other statistical theories of measurements. It seems that this principle might play in quantum theory the role that is similar to the role of Einstein's relativity principle.

  10. A Systematic Evaluation of Blood Serum and Plasma Pre-Analytics for Metabolomics Cohort Studies

    Directory of Open Access Journals (Sweden)

    Elodie Jobard

    2016-12-01

    Full Text Available The recent thriving development of biobanks and associated high-throughput phenotyping studies requires the elaboration of large-scale approaches for monitoring biological sample quality and compliance with standard protocols. We present a metabolomic investigation of human blood samples that delineates pitfalls and guidelines for the collection, storage and handling procedures for serum and plasma. A series of eight pre-processing technical parameters is systematically investigated along variable ranges commonly encountered across clinical studies. While metabolic fingerprints, as assessed by nuclear magnetic resonance, are not significantly affected by altered centrifugation parameters or delays between sample pre-processing (blood centrifugation and storage, our metabolomic investigation highlights that both the delay and storage temperature between blood draw and centrifugation are the primary parameters impacting serum and plasma metabolic profiles. Storing the blood drawn at 4 °C is shown to be a reliable routine to confine variability associated with idle time prior to sample pre-processing. Based on their fine sensitivity to pre-analytical parameters and protocol variations, metabolic fingerprints could be exploited as valuable ways to determine compliance with standard procedures and quality assessment of blood samples within large multi-omic clinical and translational cohort studies.

  11. Management of thyroid cytological material, pre-analytical procedures and bio-banking.

    Science.gov (United States)

    Bode-Lesniewska, Beata; Cochand-Priollet, Beatrix; Straccia, Patrizia; Fadda, Guido; Bongiovanni, Massimo

    2018-06-09

    Thyroid nodules are common and increasingly detected due to recent advances in imaging techniques. However, clinically relevant thyroid cancer is rare and the mortality from aggressive thyroid cancer remains constant. FNAC (Fine Needle Aspiration Cytology) is a standard method for diagnosing thyroid malignancy and the discrimination of malignant nodules from goiter. As the examined nodules on thyroid FNAC are often small incidental findings, it is important to maintain a low rate of undetermined diagnoses requiring further clinical work up or surgery. The most important factors determining the accuracy of the cytological diagnosis and suitability for biobanking of thyroid FNACs are the quality of the sample and availability of adequate tissue for auxiliary studies. This article analyses technical aspects (pre-analytics) of performing thyroid FNACs, including image guidance and rapid on slide evaluation (ROSE), sample collection methods (conventional slides, liquid based methods (LBC), cell blocks) and storage (bio-banking). The spectrum of the special studies (immunocytochemistry on direct slides or LBC, immunohistochemistry on cell blocks and molecular methods) required for improving the precision of the cytological diagnosis of the thyroid nodules is discussed. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.

  12. Differences in metabolite profiles caused by pre-analytical blood processing procedures.

    Science.gov (United States)

    Nishiumi, Shin; Suzuki, Makoto; Kobayashi, Takashi; Yoshida, Masaru

    2018-05-01

    Recently, the use of metabolomic analysis of human serum and plasma for biomarker discovery and disease diagnosis in clinical studies has been increasing. The feasibility of using a metabolite biomarker for disease diagnosis is strongly dependent on the metabolite's stability during pre-analytical blood processing procedures, such as serum or plasma sampling and sample storage prior to centrifugation. However, the influence of blood processing procedures on the stability of metabolites has not been fully characterized. In the present study, we compared the levels of metabolites in matched human serum and plasma samples using gas chromatography coupled with mass spectrometry and liquid chromatography coupled with mass spectrometry. In addition, we evaluated the changes in plasma metabolite levels induced by storage at room temperature or at a cold temperature prior to centrifugation. As a result, it was found that 76 metabolites exhibited significant differences between their serum and plasma levels. Furthermore, the pre-centrifugation storage conditions significantly affected the plasma levels of 45 metabolites. These results highlight the importance of blood processing procedures during metabolome analysis, which should be considered during biomarker discovery and the subsequent use of biomarkers for disease diagnosis. Copyright © 2017 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.

  13. Integrating the Carbon and Water Footprints’ Costs in the Water Framework Directive 2000/60/EC Full Water Cost Recovery Concept: Basic Principles Towards Their Reliable Calculation and Socially Just Allocation

    Directory of Open Access Journals (Sweden)

    Anastasia Papadopoulou

    2012-01-01

    Full Text Available This paper presents the basic principles for the integration of the water and carbon footprints cost into the resource and environmental costs respectively, taking the suggestions set by the Water Framework Directive (WFD 2000/60/EC one step forward. WFD states that full water cost recovery (FWCR should be based on the estimation of the three sub-costs related: direct; environmental; and resource cost. It also strongly suggests the EU Member States develop and apply effective water pricing policies to achieve FWCR. These policies must be socially just to avoid any social injustice phenomena. This is a very delicate task to handle, especially within the fragile economic conditions that the EU is facing today. Water losses play a crucial role for the FWC estimation. Water losses should not be neglected since they are one of the major “water uses” in any water supply network. A methodology is suggested to reduce water losses and the related Non Revenue Water (NRW index. An Expert Decision Support System is proposed to assess the FWC incorporating the Water and Carbon Footprint costs.

  14. Hygiene Basics

    Science.gov (United States)

    ... Staying Safe Videos for Educators Search English Español Hygiene Basics KidsHealth / For Teens / Hygiene Basics What's in this article? Oily Hair Sweat ... smell, anyway? Read below for information on some hygiene basics — and learn how to deal with greasy ...

  15. Bias due to Preanalytical Dilution of Rodent Serum for Biochemical Analysis on the Siemens Dimension Xpand Plus

    Directory of Open Access Journals (Sweden)

    Jennifer L. Johns

    2018-02-01

    Full Text Available Clinical pathology testing of rodents is often challenging due to insufficient sample volume. One solution in clinical veterinary and exploratory research environments is dilution of samples prior to analysis. However, published information on the impact of preanalytical sample dilution on rodent biochemical data is incomplete. The objective of this study was to evaluate the effects of preanalytical sample dilution on biochemical analysis of mouse and rat serum samples utilizing the Siemens Dimension Xpand Plus. Rats were obtained from end of study research projects. Mice were obtained from sentinel testing programs. For both, whole blood was collected via terminal cardiocentesis into empty tubes and serum was harvested. Biochemical parameters were measured on fresh and thawed frozen samples run straight and at dilution factors 2–10. Dilutions were performed manually, utilizing either ultrapure water or enzyme diluent per manufacturer recommendations. All diluted samples were generated directly from the undiluted sample. Preanalytical dilution caused clinically unacceptable bias in most analytes at dilution factors four and above. Dilution-induced bias in total calcium, creatinine, total bilirubin, and uric acid was considered unacceptable with any degree of dilution, based on the more conservative of two definitions of acceptability. Dilution often caused electrolyte values to fall below assay range precluding evaluation of bias. Dilution-induced bias occurred in most biochemical parameters to varying degrees and may render dilution unacceptable in the exploratory research and clinical veterinary environments. Additionally, differences between results obtained at different dilution factors may confound statistical comparisons in research settings. Comparison of data obtained at a single dilution factor is highly recommended.

  16. Pre-analytical sample quality: metabolite ratios as an intrinsic marker for prolonged room temperature exposure of serum samples.

    Directory of Open Access Journals (Sweden)

    Gabriele Anton

    Full Text Available Advances in the "omics" field bring about the need for a high number of good quality samples. Many omics studies take advantage of biobanked samples to meet this need. Most of the laboratory errors occur in the pre-analytical phase. Therefore evidence-based standard operating procedures for the pre-analytical phase as well as markers to distinguish between 'good' and 'bad' quality samples taking into account the desired downstream analysis are urgently needed. We studied concentration changes of metabolites in serum samples due to pre-storage handling conditions as well as due to repeated freeze-thaw cycles. We collected fasting serum samples and subjected aliquots to up to four freeze-thaw cycles and to pre-storage handling delays of 12, 24 and 36 hours at room temperature (RT and on wet and dry ice. For each treated aliquot, we quantified 127 metabolites through a targeted metabolomics approach. We found a clear signature of degradation in samples kept at RT. Storage on wet ice led to less pronounced concentration changes. 24 metabolites showed significant concentration changes at RT. In 22 of these, changes were already visible after only 12 hours of storage delay. Especially pronounced were increases in lysophosphatidylcholines and decreases in phosphatidylcholines. We showed that the ratio between the concentrations of these molecule classes could serve as a measure to distinguish between 'good' and 'bad' quality samples in our study. In contrast, we found quite stable metabolite concentrations during up to four freeze-thaw cycles. We concluded that pre-analytical RT handling of serum samples should be strictly avoided and serum samples should always be handled on wet ice or in cooling devices after centrifugation. Moreover, serum samples should be frozen at or below -80°C as soon as possible after centrifugation.

  17. Bernoulli's Principle

    Science.gov (United States)

    Hewitt, Paul G.

    2004-01-01

    Some teachers have difficulty understanding Bernoulli's principle particularly when the principle is applied to the aerodynamic lift. Some teachers favor using Newton's laws instead of Bernoulli's principle to explain the physics behind lift. Some also consider Bernoulli's principle too difficult to explain to students and avoid teaching it…

  18. Preanalytical and analytical variation of surface-enhanced laser desorption-ionization time-of-flight mass spectrometry of human serum

    DEFF Research Database (Denmark)

    Albrethsen, Jakob; Bøgebo, Rikke; Olsen, Jesper

    2006-01-01

    BACKGROUND: Surface-enhanced laser desorption-ionization time-of-flight (SELDI-TOF) mass spectrometry of human serum is a potential diagnostic tool in human diseases. In the present study, the preanalytical and analytical variation of SELDI-TOF mass spectrometry of serum was assessed in healthy...... was 18% (6%-34%, n=4) for 16 peaks, and inter-individual CV was 38% (16%-56%, n=16) for 20 peaks. CONCLUSIONS: The pre-analytical and analytical conditions of SELDI-TOF mass spectrometry of serum have a significant impact on the protein peaks, with the number of peaks low and the assay variation high...

  19. Pre-analytical and analytical aspects affecting clinical reliability of plasma glucose results.

    Science.gov (United States)

    Pasqualetti, Sara; Braga, Federica; Panteghini, Mauro

    2017-07-01

    The measurement of plasma glucose (PG) plays a central role in recognizing disturbances in carbohydrate metabolism, with established decision limits that are globally accepted. This requires that PG results are reliable and unequivocally valid no matter where they are obtained. To control the pre-analytical variability of PG and prevent in vitro glycolysis, the use of citrate as rapidly effective glycolysis inhibitor has been proposed. However, the commercial availability of several tubes with studies showing different performance has created confusion among users. Moreover, and more importantly, studies have shown that tubes promptly inhibiting glycolysis give PG results that are significantly higher than tubes containing sodium fluoride only, used in the majority of studies generating the current PG cut-points, with a different clinical classification of subjects. From the analytical point of view, to be equivalent among different measuring systems, PG results should be traceable to a recognized higher-order reference via the implementation of an unbroken metrological hierarchy. In doing this, it is important that manufacturers of measuring systems consider the uncertainty accumulated through the different steps of the selected traceability chain. In particular, PG results should fulfil analytical performance specifications defined to fit the intended clinical application. Since PG has tight homeostatic control, its biological variability may be used to define these limits. Alternatively, given the central diagnostic role of the analyte, an outcome model showing the impact of analytical performance of test on clinical classifications of subjects can be used. Using these specifications, performance assessment studies employing commutable control materials with values assigned by reference procedure have shown that the quality of PG measurements is often far from desirable and that problems are exacerbated using point-of-care devices. Copyright © 2017 The Canadian

  20. [Bioethics of principles].

    Science.gov (United States)

    Pérez-Soba Díez del Corral, Juan José

    2008-01-01

    Bioethics emerges about the tecnological problems of acting in human life. Emerges also the problem of the moral limits determination, because they seem exterior of this practice. The Bioethics of Principles, take his rationality of the teleological thinking, and the autonomism. These divergence manifest the epistemological fragility and the great difficulty of hmoralñ thinking. This is evident in the determination of autonomy's principle, it has not the ethical content of Kant's propose. We need a new ethic rationality with a new refelxion of new Principles whose emerges of the basic ethic experiences.

  1. Basic electrotechnology

    CERN Document Server

    Ashen, R A

    2013-01-01

    BASIC Electrotechnology discusses the applications of Beginner's All-purpose Symbolic Instruction Code (BASIC) in engineering, particularly in solving electrotechnology-related problems. The book is comprised of six chapters that cover several topics relevant to BASIC and electrotechnology. Chapter 1 provides an introduction to BASIC, and Chapter 2 talks about the use of complex numbers in a.c. circuit analysis. Chapter 3 covers linear circuit analysis with d.c. and sinusoidal a.c. supplies. The book also discusses the elementary magnetic circuit theory. The theory and performance of two windi

  2. Back to basics audio

    CERN Document Server

    Nathan, Julian

    1998-01-01

    Back to Basics Audio is a thorough, yet approachable handbook on audio electronics theory and equipment. The first part of the book discusses electrical and audio principles. Those principles form a basis for understanding the operation of equipment and systems, covered in the second section. Finally, the author addresses planning and installation of a home audio system.Julian Nathan joined the audio service and manufacturing industry in 1954 and moved into motion picture engineering and production in 1960. He installed and operated recording theaters in Sydney, Austra

  3. Variational principles in physics

    CERN Document Server

    Basdevant, Jean-Louis

    2007-01-01

    Optimization under constraints is an essential part of everyday life. Indeed, we routinely solve problems by striking a balance between contradictory interests, individual desires and material contingencies. This notion of equilibrium was dear to thinkers of the enlightenment, as illustrated by Montesquieu’s famous formulation: "In all magistracies, the greatness of the power must be compensated by the brevity of the duration." Astonishingly, natural laws are guided by a similar principle. Variational principles have proven to be surprisingly fertile. For example, Fermat used variational methods to demonstrate that light follows the fastest route from one point to another, an idea which came to be known as Fermat’s principle, a cornerstone of geometrical optics. Variational Principles in Physics explains variational principles and charts their use throughout modern physics. The heart of the book is devoted to the analytical mechanics of Lagrange and Hamilton, the basic tools of any physicist. Prof. Basdev...

  4. Anesthesia Basics

    Science.gov (United States)

    ... Staying Safe Videos for Educators Search English Español Anesthesia Basics KidsHealth / For Teens / Anesthesia Basics What's in ... español Conceptos básicos sobre la anestesia What Is Anesthesia? No doubt about it, getting an operation can ...

  5. BASIC Programming.

    Science.gov (United States)

    Jennings, Carol Ann

    Designed for use by both secondary- and postsecondary-level business teachers, this curriculum guide consists of 10 units of instructional materials dealing with Beginners All-Purpose Symbol Instruction Code (BASIC) programing. Topics of the individual lessons are numbering BASIC programs and using the PRINT, END, and REM statements; system…

  6. Basics of statistical physics

    CERN Document Server

    Müller-Kirsten, Harald J W

    2013-01-01

    Statistics links microscopic and macroscopic phenomena, and requires for this reason a large number of microscopic elements like atoms. The results are values of maximum probability or of averaging. This introduction to statistical physics concentrates on the basic principles, and attempts to explain these in simple terms supplemented by numerous examples. These basic principles include the difference between classical and quantum statistics, a priori probabilities as related to degeneracies, the vital aspect of indistinguishability as compared with distinguishability in classical physics, the differences between conserved and non-conserved elements, the different ways of counting arrangements in the three statistics (Maxwell-Boltzmann, Fermi-Dirac, Bose-Einstein), the difference between maximization of the number of arrangements of elements, and averaging in the Darwin-Fowler method. Significant applications to solids, radiation and electrons in metals are treated in separate chapters, as well as Bose-Eins...

  7. Standard operating procedures for pre-analytical handling of blood and urine for metabolomic studies and biobanks.

    Science.gov (United States)

    Bernini, Patrizia; Bertini, Ivano; Luchinat, Claudio; Nincheri, Paola; Staderini, Samuele; Turano, Paola

    2011-04-01

    (1)H NMR metabolic profiling of urine, serum and plasma has been used to monitor the impact of the pre-analytical steps on the sample quality and stability in order to propose standard operating procedures (SOPs) for deposition in biobanks. We analyzed the quality of serum and plasma samples as a function of the elapsed time (t = 0-4 h) between blood collection and processing and of the time from processing to freezing (up to 24 h). The stability of the urine metabolic profile over time (up to 24 h) at various storage temperatures was monitored as a function of the different pre-analytical treatments like pre-storage centrifugation, filtration, and addition of the bacteriostatic preservative sodium azide. Appreciable changes in the profiles, reflecting changes in the concentration of a number of metabolites, were detected and discussed in terms of chemical and enzymatic reactions for both blood and urine samples. Appropriate procedures for blood derivatives collection and urine preservation/storage that allow maintaining as much as possible the original metabolic profile of the fresh samples emerge, and are proposed as SOPs for biobanking.

  8. Standard operating procedures for pre-analytical handling of blood and urine for metabolomic studies and biobanks

    International Nuclear Information System (INIS)

    Bernini, Patrizia; Bertini, Ivano; Luchinat, Claudio; Nincheri, Paola; Staderini, Samuele; Turano, Paola

    2011-01-01

    1 H NMR metabolic profiling of urine, serum and plasma has been used to monitor the impact of the pre-analytical steps on the sample quality and stability in order to propose standard operating procedures (SOPs) for deposition in biobanks. We analyzed the quality of serum and plasma samples as a function of the elapsed time (t = 0−4 h) between blood collection and processing and of the time from processing to freezing (up to 24 h). The stability of the urine metabolic profile over time (up to 24 h) at various storage temperatures was monitored as a function of the different pre-analytical treatments like pre-storage centrifugation, filtration, and addition of the bacteriostatic preservative sodium azide. Appreciable changes in the profiles, reflecting changes in the concentration of a number of metabolites, were detected and discussed in terms of chemical and enzymatic reactions for both blood and urine samples. Appropriate procedures for blood derivatives collection and urine preservation/storage that allow maintaining as much as possible the original metabolic profile of the fresh samples emerge, and are proposed as SOPs for biobanking.

  9. Analysis of Pre-Analytic Factors Affecting the Success of Clinical Next-Generation Sequencing of Solid Organ Malignancies

    International Nuclear Information System (INIS)

    Chen, Hui; Luthra, Rajyalakshmi; Goswami, Rashmi S.; Singh, Rajesh R.; Roy-Chowdhuri, Sinchita

    2015-01-01

    Application of next-generation sequencing (NGS) technology to routine clinical practice has enabled characterization of personalized cancer genomes to identify patients likely to have a response to targeted therapy. The proper selection of tumor sample for downstream NGS based mutational analysis is critical to generate accurate results and to guide therapeutic intervention. However, multiple pre-analytic factors come into play in determining the success of NGS testing. In this review, we discuss pre-analytic requirements for AmpliSeq PCR-based sequencing using Ion Torrent Personal Genome Machine (PGM) (Life Technologies), a NGS sequencing platform that is often used by clinical laboratories for sequencing solid tumors because of its low input DNA requirement from formalin fixed and paraffin embedded tissue. The success of NGS mutational analysis is affected not only by the input DNA quantity but also by several other factors, including the specimen type, the DNA quality, and the tumor cellularity. Here, we review tissue requirements for solid tumor NGS based mutational analysis, including procedure types, tissue types, tumor volume and fraction, decalcification, and treatment effects

  10. Technical evaluation of the novel preanalytical module on instrumentation laboratory ACL TOP: advancing automation in hemostasis testing.

    Science.gov (United States)

    Lippi, Giuseppe; Ippolito, Luigi; Favaloro, Emmanuel J

    2013-10-01

    Automation in hemostasis testing is entering an exciting and unprecedented phase. This study was planned to assess the performance of the new preanalytical module on the hemostasis testing system Instrumentation Laboratory ACL TOP. The evaluation included interference studies to define reliable thresholds for rejecting samples with significant concentrations of interfering substances; within-run imprecision studies of plasma indices on four different interference degrees for each index; comparison studies with reference measures of hemolysis index, bilirubin, and triglycerides on clinical chemistry analyzers; and calculation of turnaround time with and without automatic performance of preanalytical check. The upper limits for sample rejection according to our interference studies were 3.6 g/L for hemoglobin, 13.6 mg/dL for bilirubin, and 1454 mg/dL for triglycerides. We found optimal precision for all indices (0.6% to 3.1% at clinically relevant thresholds) and highly significant correlations with reference measures on clinical chemistry analyzers (from 0.985 to 0.998). The limited increase of turnaround time (i.e., +3% and +5% with or without cap-piercing), coupled with no adjunctive costs over performance of normal coagulation assays, contribute to make the automatic check of plasma indices on ACL TOP a reliable and practical approach for improving testing quality and safeguarding patient safety.

  11. Standard operating procedures for pre-analytical handling of blood and urine for metabolomic studies and biobanks

    Energy Technology Data Exchange (ETDEWEB)

    Bernini, Patrizia; Bertini, Ivano, E-mail: bertini@cerm.unifi.it; Luchinat, Claudio [University of Florence, Magnetic Resonance Center (CERM) (Italy); Nincheri, Paola; Staderini, Samuele [FiorGen Foundation (Italy); Turano, Paola [University of Florence, Magnetic Resonance Center (CERM) (Italy)

    2011-04-15

    {sup 1}H NMR metabolic profiling of urine, serum and plasma has been used to monitor the impact of the pre-analytical steps on the sample quality and stability in order to propose standard operating procedures (SOPs) for deposition in biobanks. We analyzed the quality of serum and plasma samples as a function of the elapsed time (t = 0-4 h) between blood collection and processing and of the time from processing to freezing (up to 24 h). The stability of the urine metabolic profile over time (up to 24 h) at various storage temperatures was monitored as a function of the different pre-analytical treatments like pre-storage centrifugation, filtration, and addition of the bacteriostatic preservative sodium azide. Appreciable changes in the profiles, reflecting changes in the concentration of a number of metabolites, were detected and discussed in terms of chemical and enzymatic reactions for both blood and urine samples. Appropriate procedures for blood derivatives collection and urine preservation/storage that allow maintaining as much as possible the original metabolic profile of the fresh samples emerge, and are proposed as SOPs for biobanking.

  12. Thyroglobulin assay in fluids from lymph node fine needle-aspiration washout: influence of pre-analytical conditions.

    Science.gov (United States)

    Casson, Florence Boux de; Moal, Valérie; Gauchez, Anne-Sophie; Moineau, Marie-Pierre; Sault, Corinne; Schlageter, Marie-Hélène; Massart, Catherine

    2017-04-01

    The aim of this study was to evaluate the pre-analytical factors contributing to uncertainty in thyroglobulin measurement in fluids from fine-needle aspiration (FNA) washout of cervical lymph nodes. We studied pre-analytical stability, in different conditions, of 41 samples prepared with concentrated solutions of thyroglobulin (FNA washout or certified standard) diluted in physiological saline solution or buffer containing 6% albumin. In this buffer, over time, no changes in thyroglobulin concentrations were observed in all storage conditions tested. In albumin free saline solution, thyroglobulin recovery rates depended on initial sample concentrations and on modalities of their conservation (in conventional storage tubes, recovery mean was 56% after 3 hours-storage at room temperature and 19% after 24 hours-storage for concentrations ranged from 2 to 183 μg/L; recovery was 95%, after 3 hours or 24 hours-storage at room temperature, for a concentration of 5,656 μg/L). We show here that these results are due to non-specific adsorption of thyroglobulin in storage tubes, which depends on sample protein concentrations. We also show that possible contamination of fluids from FNA washout by plasma proteins do not always adequately prevent this adsorption. In conclusion, non-specific adsorption in storage tubes strongly contributes to uncertainty in thyroglobulin measurement in physiological saline solution. It is therefore recommended, for FNA washout, to use a buffer containing proteins provided by the laboratory.

  13. Analysis of Pre-Analytic Factors Affecting the Success of Clinical Next-Generation Sequencing of Solid Organ Malignancies

    Energy Technology Data Exchange (ETDEWEB)

    Chen, Hui [Department of Pathology, The University of Texas MD Anderson Cancer Center, 1515 Holcombe Blvd, Houston, TX 77030 (United States); Luthra, Rajyalakshmi, E-mail: rluthra@mdanderson.org; Goswami, Rashmi S.; Singh, Rajesh R. [Department of Hematopathology, The University of Texas MD Anderson Cancer Center, 1515 Holcombe Blvd, Houston, TX 77030 (United States); Roy-Chowdhuri, Sinchita [Department of Pathology, The University of Texas MD Anderson Cancer Center, 1515 Holcombe Blvd, Houston, TX 77030 (United States)

    2015-08-28

    Application of next-generation sequencing (NGS) technology to routine clinical practice has enabled characterization of personalized cancer genomes to identify patients likely to have a response to targeted therapy. The proper selection of tumor sample for downstream NGS based mutational analysis is critical to generate accurate results and to guide therapeutic intervention. However, multiple pre-analytic factors come into play in determining the success of NGS testing. In this review, we discuss pre-analytic requirements for AmpliSeq PCR-based sequencing using Ion Torrent Personal Genome Machine (PGM) (Life Technologies), a NGS sequencing platform that is often used by clinical laboratories for sequencing solid tumors because of its low input DNA requirement from formalin fixed and paraffin embedded tissue. The success of NGS mutational analysis is affected not only by the input DNA quantity but also by several other factors, including the specimen type, the DNA quality, and the tumor cellularity. Here, we review tissue requirements for solid tumor NGS based mutational analysis, including procedure types, tissue types, tumor volume and fraction, decalcification, and treatment effects.

  14. Analysis of Pre-Analytic Factors Affecting the Success of Clinical Next-Generation Sequencing of Solid Organ Malignancies

    Directory of Open Access Journals (Sweden)

    Hui Chen

    2015-08-01

    Full Text Available Application of next-generation sequencing (NGS technology to routine clinical practice has enabled characterization of personalized cancer genomes to identify patients likely to have a response to targeted therapy. The proper selection of tumor sample for downstream NGS based mutational analysis is critical to generate accurate results and to guide therapeutic intervention. However, multiple pre-analytic factors come into play in determining the success of NGS testing. In this review, we discuss pre-analytic requirements for AmpliSeq PCR-based sequencing using Ion Torrent Personal Genome Machine (PGM (Life Technologies, a NGS sequencing platform that is often used by clinical laboratories for sequencing solid tumors because of its low input DNA requirement from formalin fixed and paraffin embedded tissue. The success of NGS mutational analysis is affected not only by the input DNA quantity but also by several other factors, including the specimen type, the DNA quality, and the tumor cellularity. Here, we review tissue requirements for solid tumor NGS based mutational analysis, including procedure types, tissue types, tumor volume and fraction, decalcification, and treatment effects.

  15. Impact of pre-analytical factors on the proteomic analysis of formalin-fixed paraffin-embedded tissue.

    Science.gov (United States)

    Thompson, Seonaid M; Craven, Rachel A; Nirmalan, Niroshini J; Harnden, Patricia; Selby, Peter J; Banks, Rosamonde E

    2013-04-01

    Formalin-fixed paraffin-embedded (FFPE) tissue samples represent a tremendous potential resource for biomarker discovery, with large numbers of samples in hospital pathology departments and links to clinical information. However, the cross-linking of proteins and nucleic acids by formalin fixation has hampered analysis and proteomic studies have been restricted to using frozen tissue, which is more limited in availability as it needs to be collected specifically for research. This means that rare disease subtypes cannot be studied easily. Recently, improved extraction techniques have enabled analysis of FFPE tissue by a number of proteomic techniques. As with all clinical samples, pre-analytical factors are likely to impact on the results obtained, although overlooked in many studies. The aim of this review is to discuss the various pre-analytical factors, which include warm and cold ischaemic time, size of sample, fixation duration and temperature, tissue processing conditions, length of storage of archival tissue and storage conditions, and to review the studies that have considered these factors in more detail. In those areas where investigations are few or non-existent, illustrative examples of the possible importance of specific factors have been drawn from studies using frozen tissue or from immunohistochemical studies of FFPE tissue. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  16. Principles of Bridge Reliability

    DEFF Research Database (Denmark)

    Thoft-Christensen, Palle; Nowak, Andrzej S.

    The paper gives a brief introduction to the basic principles of structural reliability theory and its application to bridge engineering. Fundamental concepts like failure probability and reliability index are introduced. Ultimate as well as serviceability limit states for bridges are formulated......, and as an example the reliability profile and a sensitivity analyses for a corroded reinforced concrete bridge is shown....

  17. The Pauli Exclusion Principle

    Indian Academy of Sciences (India)

    his exclusion principle, the quantum theory was a mess. Moreover, it could ... This is a function of all the coordinates and 'internal variables' such as spin, of all the ... must remain basically the same (ie change by a phase factor at most) if we ...

  18. Landauer's principle and the conservation of information

    International Nuclear Information System (INIS)

    Daffertshofer, A.; Plastino, A.R.

    2005-01-01

    Erasure of information requires the dissipation of a minimal amount of energy as being formulated in Landauer's principle. This profound concept in information processing has recently been derived by use of basic dynamical principles of statistical mechanics. We present an alternative derivation of Landauer's principle based on dynamical principles and certain properties of the Shannon-Gibbs-Boltzmann entropy, in particular (sub-)additivity

  19. Basic accelerator optics

    CERN Document Server

    CERN. Geneva. Audiovisual Unit

    1985-01-01

    A complete derivation, from first principles, of the concepts and methods applied in linear accelerator and beamline optics will be presented. Particle motion and beam motion in systems composed of linear magnets, as well as weak and strong focusing and special insertions are treated in mathematically simple terms, and design examples for magnets and systems are given. This series of five lectures is intended to provide all the basic tools required for the design and operation of beam optical systems.

  20. Principles of musical acoustics

    CERN Document Server

    Hartmann, William M

    2013-01-01

    Principles of Musical Acoustics focuses on the basic principles in the science and technology of music. Musical examples and specific musical instruments demonstrate the principles. The book begins with a study of vibrations and waves, in that order. These topics constitute the basic physical properties of sound, one of two pillars supporting the science of musical acoustics. The second pillar is the human element, the physiological and psychological aspects of acoustical science. The perceptual topics include loudness, pitch, tone color, and localization of sound. With these two pillars in place, it is possible to go in a variety of directions. The book treats in turn, the topics of room acoustics, audio both analog and digital, broadcasting, and speech. It ends with chapters on the traditional musical instruments, organized by family. The mathematical level of this book assumes that the reader is familiar with elementary algebra. Trigonometric functions, logarithms and powers also appear in the book, but co...

  1. Order of blood draw: Opinion Paper by the European Federation for Clinical Chemistry and Laboratory Medicine (EFLM) Working Group for the Preanalytical Phase (WG-PRE)

    NARCIS (Netherlands)

    Cornes, Michael; van Dongen-Lases, Edmée; Grankvist, Kjell; Ibarz, Mercedes; Kristensen, Gunn; Lippi, Giuseppe; Nybo, Mads; Simundic, Ana-Maria

    2017-01-01

    It has been well reported over recent years that most errors within the total testing process occur in the pre-analytical phase (46%-68.2%), an area that is usually outside of the direct control of the laboratory and which includes sample collection (phlebotomy). National and international (WHO,

  2. Pre-analytical and analytical variation of drug determination in segmented hair using ultra-performance liquid chromatography-tandem mass spectrometry

    DEFF Research Database (Denmark)

    Nielsen, Marie Katrine Klose; Johansen, Sys Stybe; Linnet, Kristian

    2014-01-01

    variation was estimated to be less than 15% for almost all compounds. The method was successfully applied to 25 segmented hair specimens from deceased drug addicts showing a broad pattern of poly-drug use. The pre-analytical sampling variation was estimated from the genuine duplicate measurements of two...

  3. SPIDIA-RNA: First external quality assessment for the pre-analytical phase of blood samples used for RNA based analyses

    Czech Academy of Sciences Publication Activity Database

    Pazzagli, M.; Malentacchi, F.; Simi, L.; Wyrich, R.; Guenther, K.; Hartmann, C.; Verderio, P.; Pizzamiglio, S.; Ciniselli, C.M.; Tichopád, Aleš; Kubista, Mikael; Gelmini, S.

    2013-01-01

    Roč. 59, č. 1 (2013), s. 20-31 ISSN 1046-2023 Institutional research plan: CEZ:AV0Z50520701 Keywords : Pre-analytical phase * RNA quality * Blood samples Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 3.221, year: 2013

  4. SPIDIA-DNA: An External Quality Assessment for the pre-analytical phase of blood samples used for DNA-based analyses

    Czech Academy of Sciences Publication Activity Database

    Malentacchi, F.; Pazzagli, M.; Simi, L.; Orlando, C.; Wyrich, R.; Hartmann, C.C.; Verderio, P.; Pizzamiglio, S.; Ciniselli, C.M.; Tichopád, Aleš; Kubista, Mikael; Gelmini, S.

    -, č. 424 (2013), s. 274-286 ISSN 0009-8981 Institutional research plan: CEZ:AV0Z50520701 Keywords : Pre-analytical phase * DNA quality * Blood samples Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 2.764, year: 2013

  5. Pre-analytical conditions in non-invasive prenatal testing of cell-free fetal RHD.

    Directory of Open Access Journals (Sweden)

    Frederik Banch Clausen

    Full Text Available Non-invasive prenatal testing of cell-free fetal DNA (cffDNA in maternal plasma can predict the fetal RhD type in D negative pregnant women. In Denmark, routine antenatal screening for the fetal RhD gene (RHD directs the administration of antenatal anti-D prophylaxis only to women who carry an RhD positive fetus. Prophylaxis reduces the risk of immunization that may lead to hemolytic disease of the fetus and the newborn. The reliability of predicting the fetal RhD type depends on pre-analytical factors and assay sensitivity. We evaluated the testing setup in the Capital Region of Denmark, based on data from routine antenatal RHD screening.Blood samples were drawn at gestational age 25 weeks. DNA extracted from 1 mL of plasma was analyzed for fetal RHD using a duplex method for exon 7/10. We investigated the effect of blood sample transportation time (n = 110 and ambient outdoor temperatures (n = 1539 on the levels of cffDNA and total DNA. We compared two different quantification methods, the delta Ct method and a universal standard curve. PCR pipetting was compared on two systems (n = 104.The cffDNA level was unaffected by blood sample transportation for up to 9 days and by ambient outdoor temperatures ranging from -10 °C to 28 °C during transport. The universal standard curve was applicable for cffDNA quantification. Identical levels of cffDNA were observed using the two automated PCR pipetting systems. We detected a mean of 100 fetal DNA copies/mL at a median gestational age of 25 weeks (range 10-39, n = 1317.The setup for real-time PCR-based, non-invasive prenatal testing of cffDNA in the Capital Region of Denmark is very robust. Our findings regarding the transportation of blood samples demonstrate the high stability of cffDNA. The applicability of a universal standard curve facilitates easy cffDNA quantification.

  6. Basic Finance

    Science.gov (United States)

    Vittek, J. F.

    1972-01-01

    A discussion of the basic measures of corporate financial strength, and the sources of the information is reported. Considered are: balance sheet, income statement, funds and cash flow, and financial ratios.

  7. A survey of variational principles

    International Nuclear Information System (INIS)

    Lewins, J.D.

    1993-01-01

    In this article survey of variational principles has been given. Variational principles play a significant role in mathematical theory with emphasis on the physical aspects. There are two principals used i.e. to represent the equation of the system in a succinct way and to enable a particular computation in the system to be carried out with greater accuracy. The survey of variational principles has ranged widely from its starting point in the Lagrange multiplier to optimisation principles. In an age of digital computation, these classic methods can be adapted to improve such calculations. We emphasize particularly the advantage of basic finite element methods on variational principles. (A.B.)

  8. Basic electronics

    CERN Document Server

    Holbrook, Harold D

    1971-01-01

    Basic Electronics is an elementary text designed for basic instruction in electricity and electronics. It gives emphasis on electronic emission and the vacuum tube and shows transistor circuits in parallel with electron tube circuits. This book also demonstrates how the transistor merely replaces the tube, with proper change of circuit constants as required. Many problems are presented at the end of each chapter. This book is comprised of 17 chapters and opens with an overview of electron theory, followed by a discussion on resistance, inductance, and capacitance, along with their effects on t

  9. Basic concepts

    International Nuclear Information System (INIS)

    Dorner, B.

    1999-01-01

    The basic concepts of neutron scattering as a tool for studying the structure and the dynamics of condensed matter. Theoretical aspects are outlined, the two different cases of coherent and incoherent scattering are presented. The issue of resolution, coherence volume and the role of monochromators are also discussed. (K.A.)

  10. Body Basics

    Science.gov (United States)

    ... learn more about how the body works, what basic human anatomy is, and what happens when parts of ... consult your doctor. © 1995- The Nemours Foundation. All rights reserved. Images provided by The Nemours Foundation, iStock, Getty Images, Veer, Shutterstock, and Clipart.com.

  11. Basic Thermodynamics

    International Nuclear Information System (INIS)

    Duthil, P

    2014-01-01

    The goal of this paper is to present a general thermodynamic basis that is useable in the context of superconductivity and particle accelerators. The first part recalls the purpose of thermodynamics and summarizes its important concepts. Some applications, from cryogenics to magnetic systems, are covered. In the context of basic thermodynamics, only thermodynamic equilibrium is considered

  12. Basic Thermodynamics

    Energy Technology Data Exchange (ETDEWEB)

    Duthil, P [Orsay, IPN (France)

    2014-07-01

    The goal of this paper is to present a general thermodynamic basis that is useable in the context of superconductivity and particle accelerators. The first part recalls the purpose of thermodynamics and summarizes its important concepts. Some applications, from cryogenics to magnetic systems, are covered. In the context of basic thermodynamics, only thermodynamic equilibrium is considered.

  13. Ethanol Basics

    Energy Technology Data Exchange (ETDEWEB)

    None

    2015-01-30

    Ethanol is a widely-used, domestically-produced renewable fuel made from corn and other plant materials. More than 96% of gasoline sold in the United States contains ethanol. Learn more about this alternative fuel in the Ethanol Basics Fact Sheet, produced by the U.S. Department of Energy's Clean Cities program.

  14. Principles of Optics

    Science.gov (United States)

    Born, Max; Wolf, Emil

    1999-10-01

    Principles of Optics is one of the classic science books of the twentieth century, and probably the most influential book in optics published in the past forty years. This edition has been thoroughly revised and updated, with new material covering the CAT scan, interference with broad-band light and the so-called Rayleigh-Sommerfeld diffraction theory. This edition also details scattering from inhomogeneous media and presents an account of the principles of diffraction tomography to which Emil Wolf has made a basic contribution. Several new appendices are also included. This new edition will be invaluable to advanced undergraduates, graduate students and researchers working in most areas of optics.

  15. Electrical principles 3 checkbook

    CERN Document Server

    Bird, J O

    2013-01-01

    Electrical Principles 3 Checkbook aims to introduce students to the basic electrical principles needed by technicians in electrical engineering, electronics, and telecommunications.The book first tackles circuit theorems, single-phase series A.C. circuits, and single-phase parallel A.C. circuits. Discussions focus on worked problems on parallel A.C. circuits, worked problems on series A.C. circuits, main points concerned with D.C. circuit analysis, worked problems on circuit theorems, and further problems on circuit theorems. The manuscript then examines three-phase systems and D.C. transients

  16. Wavelet basics

    CERN Document Server

    Chan, Y T

    1995-01-01

    Since the study of wavelets is a relatively new area, much of the research coming from mathematicians, most of the literature uses terminology, concepts and proofs that may, at times, be difficult and intimidating for the engineer. Wavelet Basics has therefore been written as an introductory book for scientists and engineers. The mathematical presentation has been kept simple, the concepts being presented in elaborate detail in a terminology that engineers will find familiar. Difficult ideas are illustrated with examples which will also aid in the development of an intuitive insight. Chapter 1 reviews the basics of signal transformation and discusses the concepts of duals and frames. Chapter 2 introduces the wavelet transform, contrasts it with the short-time Fourier transform and clarifies the names of the different types of wavelet transforms. Chapter 3 links multiresolution analysis, orthonormal wavelets and the design of digital filters. Chapter 4 gives a tour d'horizon of topics of current interest: wave...

  17. Education: The Basics. The Basics

    Science.gov (United States)

    Wood, Kay

    2011-01-01

    Everyone knows that education is important, we are confronted daily by discussion of it in the media and by politicians, but how much do we really know about education? "Education: The Basics" is a lively and engaging introduction to education as an academic subject, taking into account both theory and practice. Covering the schooling system, the…

  18. General Principles Governing Liability

    International Nuclear Information System (INIS)

    Reyners, P.

    1998-01-01

    This paper contains a brief review of the basic principles which govern the special regime of liability and compensation for nuclear damage originating on nuclear installations, in particular the strict and exclusive liability of the nuclear operator, the provision of a financial security to cover this liability and the limits applicable both in amount and in time. The paper also reviews the most important international agreements currently in force which constitute the foundation of this special regime. (author)

  19. Principles of lake sedimentology

    International Nuclear Information System (INIS)

    Janasson, L.

    1983-01-01

    This book presents a comprehensive outline on the basic sedimentological principles for lakes, and focuses on environmental aspects and matters related to lake management and control-on lake ecology rather than lake geology. This is a guide for those who plan, perform and evaluate lake sedimentological investigations. Contents abridged: Lake types and sediment types. Sedimentation in lakes and water dynamics. Lake bottom dynamics. Sediment dynamics and sediment age. Sediments in aquatic pollution control programmes. Subject index

  20. Basic bladder neurophysiology.

    Science.gov (United States)

    Clemens, J Quentin

    2010-11-01

    Maintenance of normal lower urinary tract function is a complex process that requires coordination between the central nervous system and the autonomic and somatic components of the peripheral nervous system. This article provides an overview of the basic principles that are recognized to regulate normal urine storage and micturition, including bladder biomechanics, relevant neuroanatomy, neural control of lower urinary tract function, and the pharmacologic processes that translate the neural signals into functional results. Finally, the emerging role of the urothelium as a sensory structure is discussed. Copyright © 2010 Elsevier Inc. All rights reserved.

  1. Circulating VEGF as a biological marker in patients with rheumatoid arthritis? Preanalytical and biological variability in healthy persons and in patients

    DEFF Research Database (Denmark)

    Hetland, Merete Lund; Christensen, Ib Jarle; Lottenburger, Tine

    2008-01-01

    /ml (range: non-detectable to 352); serum: 328 pg/ml (53-1791)) were independent of gender and age. Short- and long-term biologic variability included diurnal variation (sampling should take place after 7 AM) and impact of exercise (increased VEGF immediately after bicycling normalised within 1 hour......BACKGROUND: Soluble vascular endothelial growth factor (VEGF) is a promising biomarker in monitoring rheumatoid arthritis (RA), but studies of pre-analytical and biologic variability are few. METHODS: VEGF was measured by ELISA methods in serum and plasma from healthy persons and RA patients. Pre......). CONCLUSIONS: Pre-analytical factors and biologic variability including diurnal variation and impact of exercise should be accounted for in future studies that include circulating VEGF as a biological marker....

  2. Gamma-Ray Telescope and Uncertainty Principle

    Science.gov (United States)

    Shivalingaswamy, T.; Kagali, B. A.

    2012-01-01

    Heisenberg's Uncertainty Principle is one of the important basic principles of quantum mechanics. In most of the books on quantum mechanics, this uncertainty principle is generally illustrated with the help of a gamma ray microscope, wherein neither the image formation criterion nor the lens properties are taken into account. Thus a better…

  3. Ethical Analysis on the Phenomenon of Skipping Class in"Introduction to Basic Principles of Marxism"in Higher Vocational Colleges%高校“马克思主义基本原理概论”课逃课现象的伦理分析

    Institute of Scientific and Technical Information of China (English)

    吴晓蓉

    2015-01-01

    At present,the popular Phenomenon of Skipping Class in"Introduction to Basic Principles of Marxism"has become one of the important factors which affecting the quality of class teaching.And the learning value of utilitarianism,self-cen-tered values and the desalination of respect consciousness are deep ethical factors of skipping classes for college students. Strengthening college students'moral education is the basic way to solve the phenomenon of skipping class in "Introduction to Basic Principles of Marxism".%当前,“马克思主义基本原理概论”课逃课现象比较普遍已成为影响其课堂教学质量的一个重要因素,其中大学生功利主义的学习观、以自我为中心的价值观及尊重意识的淡化是大学生逃原理课的深层伦理因素,加强大学生道德教育是解决原理课逃课现象的基本途径。

  4. Guarani Basic Course, Part I.

    Science.gov (United States)

    Blair, Robert W.; And Others

    This is the first in a two-volume basic course in Guarani, the indigenous language of Paraguay. The volume consists of an introduction to the Guarani language, some general principles for adult language-learning, and ten instructional units. Because the goal of the course is to encourage and lead the learner to communicate in Guarani in class and…

  5. Educational Project Management Instructional System. Module Two. Project Management Basic Principles. Volume I--Lessons 1 to 6. Volume II--Lessons 7 to 12. Volume III--Case Simulation.

    Science.gov (United States)

    Cummings, C. Peter; Cook, Desmond L.

    This module is the second in a self-instructional program designed to train public school personnel in how to manage educational projects. The purpose of this module is to provide current or potential project directors with the basic knowledge, skills, abilities, and sensitivities needed to manage a local educational project. In the areas of…

  6. Increased Patient Satisfaction and a Reduction in Pre-Analytical Errors Following Implementation of an Electronic Specimen Collection Module in Outpatient Phlebotomy.

    Science.gov (United States)

    Kantartjis, Michalis; Melanson, Stacy E F; Petrides, Athena K; Landman, Adam B; Bates, David W; Rosner, Bernard A; Goonan, Ellen; Bixho, Ida; Tanasijevic, Milenko J

    2017-08-01

    Patient satisfaction in outpatient phlebotomy settings typically depends on wait time and venipuncture experience, and many patients equate their experiences with their overall satisfaction with the hospital. We compared patient service times and preanalytical errors pre- and postimplementation of an integrated electronic health record (EHR)-laboratory information system (LIS) and electronic specimen collection module. We also measured patient wait time and assessed patient satisfaction using a 5-question survey. The percentage of patients waiting less than 10 minutes increased from 86% preimplementation to 93% postimplementation of the EHR-LIS (P ≤.001). The median total service time decreased significantly, from 6 minutes (IQR, 4-8 minutes), to 5 minutes (IQR, 3-6 minutes) (P = .005). The preanalytical errors decreased significantly, from 3.20 to 1.93 errors per 1000 specimens (P ≤.001). Overall patient satisfaction improved, with an increase in excellent responses for all 5 questions (P ≤.001). We found several benefits of implementing an electronic specimen collection module, including decreased wait and service times, improved patient satisfaction, and a reduction in preanalytical errors. © American Society for Clinical Pathology, 2017. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com

  7. Optimised Pre-Analytical Methods Improve KRAS Mutation Detection in Circulating Tumour DNA (ctDNA) from Patients with Non-Small Cell Lung Cancer (NSCLC)

    Science.gov (United States)

    Sherwood, James L.; Corcoran, Claire; Brown, Helen; Sharpe, Alan D.; Musilova, Milena; Kohlmann, Alexander

    2016-01-01

    Introduction Non-invasive mutation testing using circulating tumour DNA (ctDNA) is an attractive premise. This could enable patients without available tumour sample to access more treatment options. Materials & Methods Peripheral blood and matched tumours were analysed from 45 NSCLC patients. We investigated the impact of pre-analytical variables on DNA yield and/or KRAS mutation detection: sample collection tube type, incubation time, centrifugation steps, plasma input volume and DNA extraction kits. Results 2 hr incubation time and double plasma centrifugation (2000 x g) reduced overall DNA yield resulting in lowered levels of contaminating genomic DNA (gDNA). Reduced “contamination” and increased KRAS mutation detection was observed using cell-free DNA Blood Collection Tubes (cfDNA BCT) (Streck), after 72 hrs following blood draw compared to EDTA tubes. Plasma input volume and use of different DNA extraction kits impacted DNA yield. Conclusion This study demonstrated that successful ctDNA recovery for mutation detection in NSCLC is dependent on pre-analytical steps. Development of standardised methods for the detection of KRAS mutations from ctDNA specimens is recommended to minimise the impact of pre-analytical steps on mutation detection rates. Where rapid sample processing is not possible the use of cfDNA BCT tubes would be advantageous. PMID:26918901

  8. Optimised Pre-Analytical Methods Improve KRAS Mutation Detection in Circulating Tumour DNA (ctDNA from Patients with Non-Small Cell Lung Cancer (NSCLC.

    Directory of Open Access Journals (Sweden)

    James L Sherwood

    Full Text Available Non-invasive mutation testing using circulating tumour DNA (ctDNA is an attractive premise. This could enable patients without available tumour sample to access more treatment options.Peripheral blood and matched tumours were analysed from 45 NSCLC patients. We investigated the impact of pre-analytical variables on DNA yield and/or KRAS mutation detection: sample collection tube type, incubation time, centrifugation steps, plasma input volume and DNA extraction kits.2 hr incubation time and double plasma centrifugation (2000 x g reduced overall DNA yield resulting in lowered levels of contaminating genomic DNA (gDNA. Reduced "contamination" and increased KRAS mutation detection was observed using cell-free DNA Blood Collection Tubes (cfDNA BCT (Streck, after 72 hrs following blood draw compared to EDTA tubes. Plasma input volume and use of different DNA extraction kits impacted DNA yield.This study demonstrated that successful ctDNA recovery for mutation detection in NSCLC is dependent on pre-analytical steps. Development of standardised methods for the detection of KRAS mutations from ctDNA specimens is recommended to minimise the impact of pre-analytical steps on mutation detection rates. Where rapid sample processing is not possible the use of cfDNA BCT tubes would be advantageous.

  9. Basic research projects

    International Nuclear Information System (INIS)

    1979-04-01

    The research programs under the cognizance of the Office of Energy Research (OER) are directed toward discovery of natural laws and new knowledge, and to improved understanding of the physical and biological sciences as related to the development, use, and control of energy. The ultimate goal is to develop a scientific underlay for the overall DOE effort and the fundamental principles of natural phenomena so that these phenomena may be understood, and new principles, formulated. The DOE-OER outlay activities include three major programs: High Energy Physics, Nuclear Physics, and Basic Energy Sciences. Taken together, these programs represent some 30 percent of the Nation's Federal support of basic research in the energy sciences. The research activities of OER involve more than 6,000 scientists and engineers working in some 17 major Federal Research Centers and at more than 135 different universities and industrial firms throughout the United States. Contract holders in the areas of high-energy physics, nuclear physics, materials sciences, nuclear science, chemical sciences, engineering, mathematics geosciences, advanced energy projects, and biological energy research are listed. Funding trends for recent years are outlined

  10. Variational principles

    CERN Document Server

    Moiseiwitsch, B L

    2004-01-01

    This graduate-level text's primary objective is to demonstrate the expression of the equations of the various branches of mathematical physics in the succinct and elegant form of variational principles (and thereby illuminate their interrelationship). Its related intentions are to show how variational principles may be employed to determine the discrete eigenvalues for stationary state problems and to illustrate how to find the values of quantities (such as the phase shifts) that arise in the theory of scattering. Chapter-by-chapter treatment consists of analytical dynamics; optics, wave mecha

  11. Inflation Basics

    Energy Technology Data Exchange (ETDEWEB)

    Green, Dan [Fermi National Accelerator Lab. (FNAL), Batavia, IL (United States)

    2014-03-01

    inflation since metrical fluctuations, both scalar and tensor, are also produced in inflationary models. Thus, the time appears to be appropriate for a very basic and simple exposition of the inflationary model written from a particle physics perspective. Only the simplest scalar model will be explored because it is easy to understand and contains all the basic elements of the inflationary model.

  12. Inflation Basics

    International Nuclear Information System (INIS)

    Green, Dan

    2014-01-01

    waves imprinted on the CMB. These would be a ''smoking gun'' for inflation since metrical fluctuations, both scalar and tensor, are also produced in inflationary models. Thus, the time appears to be appropriate for a very basic and simple exposition of the inflationary model written from a particle physics perspective. Only the simplest scalar model will be explored because it is easy to understand and contains all the basic elements of the inflationary model.

  13. European and Czech principles of contract law

    OpenAIRE

    Horáková, Monika

    2008-01-01

    8 Summary European and Czech Principles of Contract Law This thesis is focused on principles of contract law. In the first instance, it explains the theoretical conception of principles of law and adverts to their status in the Czech legal system. The second Chapter of the thesis dissertates about basic principles of the Czech private law starting with the principle of freedom of contract, ends with the principles of good faith and fair dealing. The main part of the thesis deals with the Euro...

  14. Safety Principles

    Directory of Open Access Journals (Sweden)

    V. A. Grinenko

    2011-06-01

    Full Text Available The offered material in the article is picked up so that the reader could have a complete representation about concept “safety”, intrinsic characteristics and formalization possibilities. Principles and possible strategy of safety are considered. A material of the article is destined for the experts who are taking up the problems of safety.

  15. Maquet principle

    Energy Technology Data Exchange (ETDEWEB)

    Levine, R.B.; Stassi, J.; Karasick, D.

    1985-04-01

    Anterior displacement of the tibial tubercle is a well-accepted orthopedic procedure in the treatment of certain patellofemoral disorders. The radiologic appearance of surgical procedures utilizing the Maquet principle has not been described in the radiologic literature. Familiarity with the physiologic and biochemical basis for the procedure and its postoperative appearance is necessary for appropriate roentgenographic evaluation and the radiographic recognition of complications.

  16. Cosmological principle

    International Nuclear Information System (INIS)

    Wesson, P.S.

    1979-01-01

    The Cosmological Principle states: the universe looks the same to all observers regardless of where they are located. To most astronomers today the Cosmological Principle means the universe looks the same to all observers because density of the galaxies is the same in all places. A new Cosmological Principle is proposed. It is called the Dimensional Cosmological Principle. It uses the properties of matter in the universe: density (rho), pressure (p), and mass (m) within some region of space of length (l). The laws of physics require incorporation of constants for gravity (G) and the speed of light (C). After combining the six parameters into dimensionless numbers, the best choices are: 8πGl 2 rho/c 2 , 8πGl 2 rho/c 4 , and 2 Gm/c 2 l (the Schwarzchild factor). The Dimensional Cosmological Principal came about because old ideas conflicted with the rapidly-growing body of observational evidence indicating that galaxies in the universe have a clumpy rather than uniform distribution

  17. Principles of digital image synthesis

    CERN Document Server

    Glassner, Andrew S

    1995-01-01

    Image synthesis, or rendering, is a field of transformation: it changesgeometry and physics into meaningful images. Because the most popularalgorithms frequently change, it is increasingly important for researchersand implementors to have a basic understanding of the principles of imagesynthesis. Focusing on theory, Andrew Glassner provides a comprehensiveexplanation of the three core fields of study that come together to formdigital image synthesis: the human visual system, digital signalprocessing, and the interaction of matter and light. Assuming no more thana basic background in calculus,

  18. ASVCP quality assurance guidelines: control of preanalytical, analytical, and postanalytical factors for urinalysis, cytology, and clinical chemistry in veterinary laboratories.

    Science.gov (United States)

    Gunn-Christie, Rebekah G; Flatland, Bente; Friedrichs, Kristen R; Szladovits, Balazs; Harr, Kendal E; Ruotsalo, Kristiina; Knoll, Joyce S; Wamsley, Heather L; Freeman, Kathy P

    2012-03-01

    In December 2009, the American Society for Veterinary Clinical Pathology (ASVCP) Quality Assurance and Laboratory Standards committee published the updated and peer-reviewed ASVCP Quality Assurance Guidelines on the Society's website. These guidelines are intended for use by veterinary diagnostic laboratories and veterinary research laboratories that are not covered by the US Food and Drug Administration Good Laboratory Practice standards (Code of Federal Regulations Title 21, Chapter 58). The guidelines have been divided into 3 reports: (1) general analytical factors for veterinary laboratory performance and comparisons; (2) hematology, hemostasis, and crossmatching; and (3) clinical chemistry, cytology, and urinalysis. This particular report is one of 3 reports and documents recommendations for control of preanalytical, analytical, and postanalytical factors related to urinalysis, cytology, and clinical chemistry in veterinary laboratories and is adapted from sections 1.1 and 2.2 (clinical chemistry), 1.3 and 2.5 (urinalysis), 1.4 and 2.6 (cytology), and 3 (postanalytical factors important in veterinary clinical pathology) of these guidelines. These guidelines are not intended to be all-inclusive; rather, they provide minimal guidelines for quality assurance and quality control for veterinary laboratory testing and a basis for laboratories to assess their current practices, determine areas for improvement, and guide continuing professional development and education efforts. © 2012 American Society for Veterinary Clinical Pathology.

  19. Quality assurance in the pre-analytical phase of human urine samples by (1)H NMR spectroscopy.

    Science.gov (United States)

    Budde, Kathrin; Gök, Ömer-Necmi; Pietzner, Maik; Meisinger, Christine; Leitzmann, Michael; Nauck, Matthias; Köttgen, Anna; Friedrich, Nele

    2016-01-01

    Metabolomic approaches investigate changes in metabolite profiles, which may reflect changes in metabolic pathways and provide information correlated with a specific biological process or pathophysiology. High-resolution (1)H NMR spectroscopy is used to identify metabolites in biofluids and tissue samples qualitatively and quantitatively. This pre-analytical study evaluated the effects of storage time and temperature on (1)H NMR spectra from human urine in two settings. Firstly, to evaluate short time effects probably due to acute delay in sample handling and secondly, the effect of prolonged storage up to one month to find markers of sample miss-handling. A number of statistical procedures were used to assess the differences between samples stored under different conditions, including Projection to Latent Structure Discriminant Analysis (PLS-DA), non-parametric testing as well as mixed effect linear regression analysis. The results indicate that human urine samples can be stored at 10 °C for 24 h or at -80 °C for 1 month, as no relevant changes in (1)H NMR fingerprints were observed during these time periods and temperature conditions. However, some metabolites most likely of microbial origin showed alterations during prolonged storage but without facilitating classification. In conclusion, the presented protocol for urine sample handling and semi-automatic metabolite quantification is suitable for large-scale epidemiological studies. Copyright © 2015 Elsevier Inc. All rights reserved.

  20. Pre-analytical and Analytical Variables Affecting the Measurement of Plasma-Derived Microparticle Tissue Factor Activity

    Science.gov (United States)

    Lee, RD; Barcel, DA; Williams, JC; Wang, JG; Boles, JC; Manly, DA; Key, NS; Mackman, N

    2011-01-01

    Introduction Elevated levels of tissue factor positive (TF+) microparticles (MPs) are observed in plasma from a variety of patients with an increased risk of thrombosis. We and others have described the measurement of TF activity in MPs isolated from plasma. The aim of this study was to investigate the effects of pre-analytical and analytical variables on TF activity of MPs isolated from blood of healthy volunteers treated ex vivo with or without bacterial lipopolysaccharide. Materials and Methods We evaluated the following parameters: use of different centrifugation speeds to isolate the MPs; comparison of TF activity of MPs isolated from platelet poor plasma versus platelet free plasma; effect of freeze/thaw on MP TF activity; and comparison of the MP TF activity assay with the measurement of TF protein by ELISA or flow cytometry. Results MPs prepared from platelet poor plasma by centrifugation at 20,000 × g or 100,000 × g for 15 minutes had similar levels of TF activity. However, significantly less TF activity was found in MPs isolated from platelet free plasma compared with platelet poor plasma. Interestingly, freeze/thawing of the plasma showed donor to donor variation in MP TF activity, with a moderate increase in some individuals. Conclusion TF+ MPs can be quantitatively isolated from platelet poor or platelet free plasma by centrifugation at 20,000 × g for 15 minutes. Measurement of MP TF activity in plasma can be used to detect a prothrombotic state in patients with various diseases. PMID:21737126

  1. Tissue is alive: New technologies are needed to address the problems of protein biomarker pre-analytical variability.

    Science.gov (United States)

    Espina, Virginia; Mueller, Claudius; Edmiston, Kirsten; Sciro, Manuela; Petricoin, Emanuel F; Liotta, Lance A

    2009-08-01

    Instability of tissue protein biomarkers is a critical issue for molecular profiling. Pre-analytical variables during tissue procurement, such as time delays during which the tissue remains stored at room temperature, can cause significant variability and bias in downstream molecular analysis. Living tissue, ex vivo, goes through a defined stage of reactive changes that begin with oxidative, hypoxic and metabolic stress, and culminate in apoptosis. Depending on the delay time ex vivo, and reactive stage, protein biomarkers, such as signal pathway phosphoproteins will be elevated or suppressed in a manner which does not represent the biomarker levels at the time of excision. Proteomic data documenting reactive tissue protein changes post collection indicate the need to recognize and address tissue stability, preservation of post-translational modifications, and preservation of morphologic features for molecular analysis. Based on the analysis of phosphoproteins, one of the most labile tissue protein biomarkers, we set forth tissue procurement guidelines for clinical research. We propose technical solutions for (i) assessing the state of protein analyte preservation and specimen quality via identification of a panel of natural proteins (surrogate stability markers), and (ii) using multi-purpose fixative solution designed to stabilize, preserve and maintain proteins, nucleic acids, and tissue architecture.

  2. MALDI-TOF MS identification of anaerobic bacteria: assessment of pre-analytical variables and specimen preparation techniques.

    Science.gov (United States)

    Hsu, Yen-Michael S; Burnham, Carey-Ann D

    2014-06-01

    Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has emerged as a tool for identifying clinically relevant anaerobes. We evaluated the analytical performance characteristics of the Bruker Microflex with Biotyper 3.0 software system for identification of anaerobes and examined the impact of direct formic acid (FA) treatment and other pre-analytical factors on MALDI-TOF MS performance. A collection of 101 anaerobic bacteria were evaluated, including Clostridium spp., Propionibacterium spp., Fusobacterium spp., Bacteroides spp., and other anaerobic bacterial of clinical relevance. The results of our study indicate that an on-target extraction with 100% FA improves the rate of accurate identification without introducing misidentification (Panaerobes grown in suboptimal conditions, such as on selective culture media and following oxygen exposure. In conclusion, we report on a number of simple and cost-effective pre- and post-analytical modifications could enhance MALDI-TOF MS identification for anaerobic bacteria. Copyright © 2014 Elsevier Inc. All rights reserved.

  3. Principles of Burn Pain Management.

    Science.gov (United States)

    James, Dominika Lipowska; Jowza, Maryam

    2017-10-01

    This article describes pathophysiology of burn injury-related pain and the basic principles of burn pain management. The focus is on concepts of perioperative and periprocedural pain management with extensive discussion of opioid-based analgesia, including patient-controlled analgesia, challenges of effective opioid therapy in opioid-tolerant patients, and opioid-induced hyperalgesia. The principles of multimodal pain management are discussed, including the importance of psychological counseling, perioperative interventional pain procedures, and alternative pain management options. A brief synopsis of the principles of outpatient pain management is provided. Copyright © 2017 Elsevier Inc. All rights reserved.

  4. Basic standards for radiation protection

    International Nuclear Information System (INIS)

    Webb, G.A.M.

    1982-01-01

    The basic standards for radiation protection have been based, for many years, on the recommendations of the International Commission of Radiological Protection. The three basic standards recommended by the Commission may be summarized as ''justification, optimization of protection and adherence to dose limitations. The applications of these basic principles to different aspects of protection are briefly summarized and the particular ways in which they have been applied to waste described in more detail. The application of dose limits, both in the control of occupational exposure and in regulating routine discharges of radioactive effluents is straight forward in principle although the measurement and calculational requirements may be substantial. Secondary standards such as derived limits may be extremely useful and the principles underlying their derivation will be described. Optimization of protection is inherently a more difficult concept to apply in protection and the various techniques used will be outlined by with particular emphasis on the use of cost benefit analysis are recommended by the ICRP. A review will be given of the problems involved in extending these basic concepts of the ICRP to probabilistic analyses such as those required for assessing the consequences of accidents or disruptive events in long term repositories. The particular difficulties posed by the very long timescales involved in the assessment of waste management practices will be discussed in some detail. (orig./RW)

  5. Core principles of evolutionary medicine

    Science.gov (United States)

    Grunspan, Daniel Z; Nesse, Randolph M; Barnes, M Elizabeth; Brownell, Sara E

    2018-01-01

    Abstract Background and objectives Evolutionary medicine is a rapidly growing field that uses the principles of evolutionary biology to better understand, prevent and treat disease, and that uses studies of disease to advance basic knowledge in evolutionary biology. Over-arching principles of evolutionary medicine have been described in publications, but our study is the first to systematically elicit core principles from a diverse panel of experts in evolutionary medicine. These principles should be useful to advance recent recommendations made by The Association of American Medical Colleges and the Howard Hughes Medical Institute to make evolutionary thinking a core competency for pre-medical education. Methodology The Delphi method was used to elicit and validate a list of core principles for evolutionary medicine. The study included four surveys administered in sequence to 56 expert panelists. The initial open-ended survey created a list of possible core principles; the three subsequent surveys winnowed the list and assessed the accuracy and importance of each principle. Results Fourteen core principles elicited at least 80% of the panelists to agree or strongly agree that they were important core principles for evolutionary medicine. These principles over-lapped with concepts discussed in other articles discussing key concepts in evolutionary medicine. Conclusions and implications This set of core principles will be helpful for researchers and instructors in evolutionary medicine. We recommend that evolutionary medicine instructors use the list of core principles to construct learning goals. Evolutionary medicine is a young field, so this list of core principles will likely change as the field develops further. PMID:29493660

  6. Principles of systems science

    CERN Document Server

    Mobus, George E

    2015-01-01

    This pioneering text provides a comprehensive introduction to systems structure, function, and modeling as applied in all fields of science and engineering. Systems understanding is increasingly recognized as a key to a more holistic education and greater problem solving skills, and is also reflected in the trend toward interdisciplinary approaches to research on complex phenomena. The subject of systems science, as a basis for understanding the components and drivers of phenomena at all scales, should be viewed with the same importance as a traditional liberal arts education. Principles of Systems Science contains many graphs, illustrations, side bars, examples, and problems to enhance understanding. From basic principles of organization, complexity, abstract representations, and behavior (dynamics) to deeper aspects such as the relations between information, knowledge, computation, and system control, to higher order aspects such as auto-organization, emergence and evolution, the book provides an integrated...

  7. Principles of Mobile Communication

    CERN Document Server

    Stüber, Gordon L

    2012-01-01

    This mathematically rigorous overview of physical layer wireless communications is now in a third, fully revised and updated edition. Along with coverage of basic principles sufficient for novice students, the volume includes plenty of finer details that will satisfy the requirements of graduate students aiming to research the topic in depth. It also has a role as a handy reference for wireless engineers. The content stresses core principles that are applicable to a broad range of wireless standards. Beginning with a survey of the field that introduces an array of issues relevant to wireless communications and which traces the historical development of today’s accepted wireless standards, the book moves on to cover all the relevant discrete subjects, from radio propagation to error probability performance and cellular radio resource management. A valuable appendix provides a succinct and focused tutorial on probability and random processes, concepts widely used throughout the book. This new edition, revised...

  8. Principles of mobile communication

    CERN Document Server

    Stüber, Gordon L

    2017-01-01

    This mathematically rigorous overview of physical layer wireless communications is now in a 4th, fully revised and updated edition. The new edition features new content on 4G cellular systems, 5G cellular outlook, bandpass signals and systems, and polarization, among many other topics, in addition to a new chapters on channel assignment techniques. Along with coverage of fundamentals and basic principles sufficient for novice students, the volume includes finer details that satisfy the requirements of graduate students aiming to conduct in-depth research. The book begins with a survey of the field, introducing issues relevant to wireless communications. The book moves on to cover relevant discrete subjects, from radio propagation, to error probability performance, and cellular radio resource management. An appendix provides a tutorial on probability and random processes. The content stresses core principles that are applicable to a broad range of wireless standards. New examples are provided throughout the bo...

  9. Principles of photonics

    CERN Document Server

    Liu, Jia-Ming

    2016-01-01

    With this self-contained and comprehensive text, students will gain a detailed understanding of the fundamental concepts and major principles of photonics. Assuming only a basic background in optics, readers are guided through key topics such as the nature of optical fields, the properties of optical materials, and the principles of major photonic functions regarding the generation, propagation, coupling, interference, amplification, modulation, and detection of optical waves or signals. Numerous examples and problems are provided throughout to enhance understanding, and a solutions manual containing detailed solutions and explanations is available online for instructors. This is the ideal resource for electrical engineering and physics undergraduates taking introductory, single-semester or single-quarter courses in photonics, providing them with the knowledge and skills needed to progress to more advanced courses on photonic devices, systems and applications.

  10. Principles of speech coding

    CERN Document Server

    Ogunfunmi, Tokunbo

    2010-01-01

    It is becoming increasingly apparent that all forms of communication-including voice-will be transmitted through packet-switched networks based on the Internet Protocol (IP). Therefore, the design of modern devices that rely on speech interfaces, such as cell phones and PDAs, requires a complete and up-to-date understanding of the basics of speech coding. Outlines key signal processing algorithms used to mitigate impairments to speech quality in VoIP networksOffering a detailed yet easily accessible introduction to the field, Principles of Speech Coding provides an in-depth examination of the

  11. Principles of quantum chemistry

    CERN Document Server

    George, David V

    2013-01-01

    Principles of Quantum Chemistry focuses on the application of quantum mechanics in physical models and experiments of chemical systems.This book describes chemical bonding and its two specific problems - bonding in complexes and in conjugated organic molecules. The very basic theory of spectroscopy is also considered. Other topics include the early development of quantum theory; particle-in-a-box; general formulation of the theory of quantum mechanics; and treatment of angular momentum in quantum mechanics. The examples of solutions of Schroedinger equations; approximation methods in quantum c

  12. Principles of fluorescence techniques

    CERN Document Server

    2016-01-01

    Fluorescence techniques are being used and applied increasingly in academics and industry. The Principles of Fluorescence Techniques course will outline the basic concepts of fluorescence techniques and the successful utilization of the currently available commercial instrumentation. The course is designed for students who utilize fluorescence techniques and instrumentation and for researchers and industrial scientists who wish to deepen their knowledge of fluorescence applications. Key scientists in the field will deliver theoretical lectures. The lectures will be complemented by the direct utilization of steady-state and lifetime fluorescence instrumentation and confocal microscopy for FLIM and FRET applications provided by leading companies.

  13. Magnetism basics and applications

    CERN Document Server

    Stefanita, Carmen-Gabriela

    2012-01-01

    This textbook is aimed at engineering students who are likely to come across magnetics applications in their professional practice. Whether designing lithography equipment containing ferromagnetic brushes, or detecting defects in aeronautics, some basic knowledge of 21st century magnetism is needed. From the magnetic tape on the pocket credit card to the read head in a personal computer, people run into magnetism in many products. Furthermore, in a variety of disciplines tools of the trade exploit magnetic principles, and many interdisciplinary laboratory research areas cross paths with magnetic phenomena that may seem mysterious to the untrained mind. Therefore, this course offers a broad coverage of magnetism topics encountered more often in this millenium, revealing key concepts on which many practical applications rest. Some traditional subjects in magnetism are discussed in the first half of the book, followed by areas likely to spark the curiosity of those more interested in today’s technological achi...

  14. Stereochemistry basic concepts and applications

    CERN Document Server

    Nógrádi, M

    2013-01-01

    Stereochemistry: Basic Concepts and Applications is a three-chapter text that introduces the basic principles and concepts of stereochemistry, as well as its application to organic chemistry application.Chapter 1 describes first the stereochemistry of the ground state, specifically the configuration and conformation of organic compounds, as well as the most important methods for its investigation. This chapter also deals with the kinetics of conformational changes and provides an overview of the so-called ""applied stereochemistry"". Chapter 2 focuses on the analysis of the internal motions of

  15. Semiconductor photocatalysis principles and applications

    CERN Document Server

    Kisch, Horst

    2014-01-01

    Focusing on the basic principles of semiconductor photocatalysis, this book also gives a brief introduction to photochemistry, photoelectrochemistry, and homogeneous photocatalysis. In addition, the author - one of the leading authorities in the field - presents important environmental and practical aspects. A valuable, one-stop source for all chemists, material scientists, and physicists working in this area, as well as novice researchers entering semiconductor photocatalysis.

  16. Ion exchange : principles and applications

    International Nuclear Information System (INIS)

    Bank, Nader; Majumdar, A.S.

    1975-01-01

    An attempt is made to provide a brief state-of-the-art review of the basic principles underlying the unit operation of ion exchange and its numerous and diverse commercial applications. A selective bibliography is provided for the benefit of the reader interested in pursuing any specific aspect of ion exchange. (author)

  17. The Oscillator Principle of Nature

    DEFF Research Database (Denmark)

    Lindberg, Erik

    2012-01-01

    Oscillators are found on all levels in Nature. The general oscillator concept is defined and investigated. Oscillators may synchronize into fractal patterns. Apparently oscillators are the basic principle in Nature. The concepts of zero and infinite are discussed. Electronic manmade oscillators...

  18. GM Food. Fundamental safety principles

    Directory of Open Access Journals (Sweden)

    Lorena GALLARDO

    2017-08-01

    Full Text Available This paper aims to provide a concise exposition of some of the most basic legal principles linked to the process of evaluation of genetically modified food safety, revealing their most salient features and also highlighting the deficiencies that some of them bring along in their application to the products under study.

  19. 2 CFR Appendix A to Part 230 - General Principles

    Science.gov (United States)

    2010-01-01

    ... 2 Grants and Agreements 1 2010-01-01 2010-01-01 false General Principles A Appendix A to Part 230 Grants and Agreements OFFICE OF MANAGEMENT AND BUDGET CIRCULARS AND GUIDANCE Reserved COST PRINCIPLES FOR... Principles General Principles Table of Contents A. Basic Considerations 1. Composition of total costs 2...

  20. Pre-analytical and analytical validations and clinical applications of a miniaturized, simple and cost-effective solid phase extraction combined with LC-MS/MS for the simultaneous determination of catecholamines and metanephrines in spot urine samples.

    Science.gov (United States)

    Li, Xiaoguang Sunny; Li, Shu; Kellermann, Gottfried

    2016-10-01

    It remains a challenge to simultaneously quantify catecholamines and metanephrines in a simple, sensitive and cost-effective manner due to pre-analytical and analytical constraints. Herein, we describe such a method consisting of a miniaturized sample preparation and selective LC-MS/MS detection by the use of second morning spot urine samples. Ten microliters of second morning urine sample were subjected to solid phase extraction on an Oasis HLB microplate upon complexation with phenylboronic acid. The analytes were well-resolved on a Luna PFP column followed by tandem mass spectrometric detection. Full validation and suitability of spot urine sampling and biological variation were investigated. The extraction recovery and matrix effect are 74.1-97.3% and 84.1-119.0%, respectively. The linearity range is 2.5-500, 0.5-500, 2.5-1250, 2.5-1250 and 0.5-1250ng/mL for norepinephrine, epinephrine, dopamine, normetanephrine and metanephrine, respectively. The intra- and inter-assay imprecisions are ≤9.4% for spiked quality control samples, and the respective recoveries are 97.2-112.5% and 95.9-104.0%. The Deming regression slope is 0.90-1.08, and the mean Bland-Altman percentage difference is from -3.29 to 11.85 between a published and proposed method (n=50). A correlation observed for the spot and 24h urine collections is significant (n=20, p<0.0001, r: 0.84-0.95, slope: 0.61-0.98). No statistical differences are found in day-to-day biological variability (n=20). Reference intervals are established for an apparently healthy population (n=88). The developed method, being practical, sensitive, reliable and cost-effective, is expected to set a new stage for routine testing, basic research and clinical applications. Copyright © 2016 Elsevier B.V. All rights reserved.

  1. Dried blood spot specimen quality and validation of a new pre-analytical processing method for qualitative HIV-1 PCR, KwaZulu-Natal, South Africa.

    Science.gov (United States)

    Govender, Kerusha; Parboosing, Raveen; Siyaca, Ntombizandile; Moodley, Pravikrishnen

    2016-01-01

    Poor quality dried blood spot (DBS) specimens are usually rejected by virology laboratories, affecting early infant diagnosis of HIV. The practice of combining two incompletely-filled DBS in one specimen preparation tube during pre-analytical specimen processing (i.e., the two-spot method) has been implemented to reduce the number of specimens being rejected for insufficient volume. This study analysed laboratory data to describe the quality of DBS specimens and the use of the two-spot method over a one-year period, then validated the two-spot method against the standard (one-spot) method. Data on HIV-1 PCR test requests submitted in 2014 to the Department of Virology at Inkosi Albert Luthuli Central Hospital in KwaZulu-Natal province, South Africa were analysed to describe reasons for specimen rejection, as well as results of the two-spot method. The accuracy, lower limit of detection and precision of the two-spot method were assessed. Of the 88 481 specimens received, 3.7% were rejected for pre-analytical problems. Of those, 48.9% were rejected as a result of insufficient specimen volume. Two health facilities had significantly more specimen rejections than other facilities. The two-spot method prevented 10 504 specimen rejections. The Pearson correlation coefficient comparing the standard to the two-spot method was 0.997. The two-spot method was comparable with the standard method of pre-analytical specimen processing. Two health facilities were identified for targeted retraining on specimen quality. The two-spot method of DBS specimen processing can be used as an adjunct to retraining, to reduce the number of specimens rejected and improve linkage to care.

  2. Pre-analytical and post-analytical evaluation in the era of molecular diagnosis of sexually transmitted diseases: cellularity control and internal control

    Directory of Open Access Journals (Sweden)

    Loria Bianchi

    2014-06-01

    Full Text Available Background. Increase of molecular tests performed on DNA extracted from various biological materials should not be carried out without an adequate standardization of the pre-analytical and post-analytical phase. Materials and Methods. Aim of this study was to evaluate the role of internal control (IC to standardize pre-analytical phase and the role of cellularity control (CC in the suitability evaluation of biological matrices, and their influence on false negative results. 120 cervical swabs (CS were pre-treated and extracted following 3 different protocols. Extraction performance was evaluated by amplification of: IC, added in each mix extraction; human gene HPRT1 (CC with RT-PCR to quantify sample cellularity; L1 region of HPV with SPF10 primers. 135 urine, 135 urethral swabs, 553 CS and 332 ThinPrep swabs (TP were tested for C. trachomatis (CT and U. parvum (UP with RT-PCR and for HPV by endpoint-PCR. Samples were also tested for cellularity. Results. Extraction protocol with highest average cellularity (Ac/sample showed lowest number of samples with inhibitors; highest HPV positivity was achieved by protocol with greatest Ac/PCR. CS and TP under 300.000 cells/sample showed a significant decrease of UP (P<0.01 and HPV (P<0.005 positivity. Female urine under 40.000 cells/mL were inadequate to detect UP (P<0.05. Conclusions. Our data show that IC and CC allow optimization of pre-analytical phase, with an increase of analytical quality. Cellularity/sample allows better sample adequacy evaluation, crucial to avoid false negative results, while cellularity/PCR allows better optimization of PCR amplification. Further data are required to define the optimal cut-off for result normalization.

  3. Dried blood spot specimen quality and validation of a new pre-analytical processing method for qualitative HIV-1 PCR, KwaZulu-Natal, South Africa

    Science.gov (United States)

    Parboosing, Raveen; Siyaca, Ntombizandile; Moodley, Pravikrishnen

    2016-01-01

    Background Poor quality dried blood spot (DBS) specimens are usually rejected by virology laboratories, affecting early infant diagnosis of HIV. The practice of combining two incompletely-filled DBS in one specimen preparation tube during pre-analytical specimen processing (i.e., the two-spot method) has been implemented to reduce the number of specimens being rejected for insufficient volume. Objectives This study analysed laboratory data to describe the quality of DBS specimens and the use of the two-spot method over a one-year period, then validated the two-spot method against the standard (one-spot) method. Methods Data on HIV-1 PCR test requests submitted in 2014 to the Department of Virology at Inkosi Albert Luthuli Central Hospital in KwaZulu-Natal province, South Africa were analysed to describe reasons for specimen rejection, as well as results of the two-spot method. The accuracy, lower limit of detection and precision of the two-spot method were assessed. Results Of the 88 481 specimens received, 3.7% were rejected for pre-analytical problems. Of those, 48.9% were rejected as a result of insufficient specimen volume. Two health facilities had significantly more specimen rejections than other facilities. The two-spot method prevented 10 504 specimen rejections. The Pearson correlation coefficient comparing the standard to the two-spot method was 0.997. Conclusions The two-spot method was comparable with the standard method of pre-analytical specimen processing. Two health facilities were identified for targeted retraining on specimen quality. The two-spot method of DBS specimen processing can be used as an adjunct to retraining, to reduce the number of specimens rejected and improve linkage to care. PMID:28879108

  4. Principle of coincidence method and application in activity measurement

    International Nuclear Information System (INIS)

    Li Mou; Dai Yihua; Ni Jianzhong

    2008-01-01

    The basic principle of coincidence method was discussed. The basic principle was generalized by analysing the actual example, and the condition in theory of coincidence method was brought forward. The cause of variation of efficiency curve and the effect of dead-time in activity measurement were explained using the above principle and condition. This principle of coincidence method provides the foundation in theory for activity measurement. (authors)

  5. Optimization of analytical and pre-analytical conditions for MALDI-TOF-MS human urine protein profiles.

    Science.gov (United States)

    Calvano, C D; Aresta, A; Iacovone, M; De Benedetto, G E; Zambonin, C G; Battaglia, M; Ditonno, P; Rutigliano, M; Bettocchi, C

    2010-03-11

    Protein analysis in biological fluids, such as urine, by means of mass spectrometry (MS) still suffers for insufficient standardization in protocols for sample collection, storage and preparation. In this work, the influence of these variables on healthy donors human urine protein profiling performed by matrix assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) was studied. A screening of various urine sample pre-treatment procedures and different sample deposition approaches on the MALDI target was performed. The influence of urine samples storage time and temperature on spectral profiles was evaluated by means of principal component analysis (PCA). The whole optimized procedure was eventually applied to the MALDI-TOF-MS analysis of human urine samples taken from prostate cancer patients. The best results in terms of detected ions number and abundance in the MS spectra were obtained by using home-made microcolumns packed with hydrophilic-lipophilic balance (HLB) resin as sample pre-treatment method; this procedure was also less expensive and suitable for high throughput analyses. Afterwards, the spin coating approach for sample deposition on the MALDI target plate was optimized, obtaining homogenous and reproducible spots. Then, PCA indicated that low storage temperatures of acidified and centrifuged samples, together with short handling time, allowed to obtain reproducible profiles without artifacts contribution due to experimental conditions. Finally, interesting differences were found by comparing the MALDI-TOF-MS protein profiles of pooled urine samples of healthy donors and prostate cancer patients. The results showed that analytical and pre-analytical variables are crucial for the success of urine analysis, to obtain meaningful and reproducible data, even if the intra-patient variability is very difficult to avoid. It has been proven how pooled urine samples can be an interesting way to make easier the comparison between

  6. Blood sample collection and patient identification demand improvement: a questionnaire study of preanalytical practices in hospital wards and laboratories.

    Science.gov (United States)

    Wallin, Olof; Söderberg, Johan; Van Guelpen, Bethany; Stenlund, Hans; Grankvist, Kjell; Brulin, Christine

    2010-09-01

    Scand J Caring Sci; 2010; 24; 581-591 
 Blood sample collection and patient identification demand improvement: a questionnaire study of preanalytical practices in hospital wards and laboratories   Most errors in venous blood testing result from human mistakes occurring before the sample reach the laboratory.   To survey venous blood sampling (VBS) practices in hospital wards and to compare practices with hospital laboratories.   Staff in two hospitals (all wards) and two hospital laboratories (314 respondents, response rate 94%), completed a questionnaire addressing issues relevant to the collection of venous blood samples for clinical chemistry testing.   The findings suggest that instructions for patient identification and the collection of venous blood samples were not always followed. For example, 79% of the respondents reported the undesirable practice (UDP) of not always using wristbands for patient identification. Similarly, 87% of the respondents noted the UDP of removing venous stasis after the sampling is finished. Compared with the ward staff, a significantly higher proportion of the laboratory staff reported desirable practices regarding the collection of venous blood samples. Neither education nor the existence of established sampling routines was clearly associated with VBS practices among the ward staff.   The results of this study, the first of its kind, suggest that a clinically important risk of error is associated with VBS in the surveyed wards. Most important is the risk of misidentification of patients. Quality improvement of blood sample collection is clearly needed, particularly in hospital wards. © 2009 The Authors. Journal compilation © 2009 Nordic College of Caring Science.

  7. Preanalytical blood sample workup for cell-free DNA analysis using Droplet Digital PCR for future molecular cancer diagnostics.

    Science.gov (United States)

    van Ginkel, Joost H; van den Broek, Daan A; van Kuik, Joyce; Linders, Dorothé; de Weger, Roel; Willems, Stefan M; Huibers, Manon M H

    2017-10-01

    In current molecular cancer diagnostics, using blood samples of cancer patients for the detection of genetic alterations in plasma (cell-free) circulating tumor DNA (ctDNA) is an emerging practice. Since ctDNA levels in blood are low, highly sensitive Droplet Digital PCR (ddPCR) can be used for detecting rare mutational targets. In order to perform ddPCR on blood samples, a standardized procedure for processing and analyzing blood samples is necessary to facilitate implementation into clinical practice. Therefore, we assessed the technical sample workup procedure for ddPCR on blood plasma samples. Blood samples from healthy individuals, as well as lung cancer patients were analyzed. We compared different methods and protocols for sample collection, storage, centrifugation, isolation, and quantification. Cell-free DNA (cfDNA) concentrations of several wild-type targets and BRAF and EGFR-mutant ctDNA concentrations quantified by ddPCR were primary outcome measurements. Highest cfDNA concentrations were measured in blood collected in serum tubes. No significant differences in cfDNA concentrations were detected between various time points of up to 24 h until centrifugation. Highest cfDNA concentrations were detected after DNA isolation with the Quick cfDNA Serum & Plasma Kit, while plasma isolation using the QIAamp Circulating Nucleic Acid Kit yielded the most consistent results. DdPCR results on cfDNA are highly dependent on multiple factors during preanalytical sample workup, which need to be addressed during the development of this diagnostic tool for cancer diagnostics in the future. © 2017 The Authors. Cancer Medicine published by John Wiley & Sons Ltd.

  8. Flow cytometry for feline lymphoma: a retrospective study about pre-analytical factors possibly affecting the quality of samples

    Directory of Open Access Journals (Sweden)

    Serena Bernardi

    2017-05-01

    Full Text Available Introduction Flow cytometry (FC is an increasingly required technique on which veterinary oncologists rely to have an accurate, fast, minimally invasive lymphoma or leukemia diagnosis. FC has been studied and applied with great results in canine oncology, whereas in feline oncology the use of this technique is still to be experienced. This is mainly due to a supposed discomfort in sampling, because of the high prevalence of intra-abdominal lymphomas. The purpose of the present study is to investigate whether any pre-analytical factor might affect the quality of suspected feline lymphoma samples for FC analysis. Methods 97 consecutive samples of suspected feline lymphoma were retrospectively selected from the authors’ institution FC database. The referring veterinarians were recalled and interrogated about several different variables, including signalling, features of the lesion, features of the sampling procedure and the experience of veterinarians performing the sampling. Statistical analyses were performed to assess the possible influence of these variables on the cellularity of the samples and the likelihood of being finally processed for FC. Results None of the investigated variables significantly influenced the quality of the submitted samples, but the needle size, with 21G needles providing the highest cellularity (Table 1. Notably, the samples quality did not vary between peripheral and intra-abdominal lesions. Sample cellularity alone influenced the likelihood of being processed. About a half of the cats required pharmacological restraint. Side effects were reported in one case only (transient swelling after peripheral lymph node sampling. Conclusions FC can be safely applied to cases of suspected feline lymphomas, even for intra-abdominal lesions. 21G needle should be preferred for sampling. This study provides the bases for the spread of this minimally invasive, fast and cost-effective technique in feline medicine.

  9. Comparison of Pre-Analytical FFPE Sample Preparation Methods and Their Impact on Massively Parallel Sequencing in Routine Diagnostics

    Science.gov (United States)

    Heydt, Carina; Fassunke, Jana; Künstlinger, Helen; Ihle, Michaela Angelika; König, Katharina; Heukamp, Lukas Carl; Schildhaus, Hans-Ulrich; Odenthal, Margarete; Büttner, Reinhard; Merkelbach-Bruse, Sabine

    2014-01-01

    Over the last years, massively parallel sequencing has rapidly evolved and has now transitioned into molecular pathology routine laboratories. It is an attractive platform for analysing multiple genes at the same time with very little input material. Therefore, the need for high quality DNA obtained from automated DNA extraction systems has increased, especially to those laboratories which are dealing with formalin-fixed paraffin-embedded (FFPE) material and high sample throughput. This study evaluated five automated FFPE DNA extraction systems as well as five DNA quantification systems using the three most common techniques, UV spectrophotometry, fluorescent dye-based quantification and quantitative PCR, on 26 FFPE tissue samples. Additionally, the effects on downstream applications were analysed to find the most suitable pre-analytical methods for massively parallel sequencing in routine diagnostics. The results revealed that the Maxwell 16 from Promega (Mannheim, Germany) seems to be the superior system for DNA extraction from FFPE material. The extracts had a 1.3–24.6-fold higher DNA concentration in comparison to the other extraction systems, a higher quality and were most suitable for downstream applications. The comparison of the five quantification methods showed intermethod variations but all methods could be used to estimate the right amount for PCR amplification and for massively parallel sequencing. Interestingly, the best results in massively parallel sequencing were obtained with a DNA input of 15 ng determined by the NanoDrop 2000c spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA). No difference could be detected in mutation analysis based on the results of the quantification methods. These findings emphasise, that it is particularly important to choose the most reliable and constant DNA extraction system, especially when using small biopsies and low elution volumes, and that all common DNA quantification techniques can be used for

  10. Comparison of pre-analytical FFPE sample preparation methods and their impact on massively parallel sequencing in routine diagnostics.

    Directory of Open Access Journals (Sweden)

    Carina Heydt

    Full Text Available Over the last years, massively parallel sequencing has rapidly evolved and has now transitioned into molecular pathology routine laboratories. It is an attractive platform for analysing multiple genes at the same time with very little input material. Therefore, the need for high quality DNA obtained from automated DNA extraction systems has increased, especially to those laboratories which are dealing with formalin-fixed paraffin-embedded (FFPE material and high sample throughput. This study evaluated five automated FFPE DNA extraction systems as well as five DNA quantification systems using the three most common techniques, UV spectrophotometry, fluorescent dye-based quantification and quantitative PCR, on 26 FFPE tissue samples. Additionally, the effects on downstream applications were analysed to find the most suitable pre-analytical methods for massively parallel sequencing in routine diagnostics. The results revealed that the Maxwell 16 from Promega (Mannheim, Germany seems to be the superior system for DNA extraction from FFPE material. The extracts had a 1.3-24.6-fold higher DNA concentration in comparison to the other extraction systems, a higher quality and were most suitable for downstream applications. The comparison of the five quantification methods showed intermethod variations but all methods could be used to estimate the right amount for PCR amplification and for massively parallel sequencing. Interestingly, the best results in massively parallel sequencing were obtained with a DNA input of 15 ng determined by the NanoDrop 2000c spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA. No difference could be detected in mutation analysis based on the results of the quantification methods. These findings emphasise, that it is particularly important to choose the most reliable and constant DNA extraction system, especially when using small biopsies and low elution volumes, and that all common DNA quantification techniques can

  11. Flow cytometry for feline lymphoma: a retrospective study regarding pre-analytical factors possibly affecting the quality of samples.

    Science.gov (United States)

    Martini, Valeria; Bernardi, Serena; Marelli, Priscilla; Cozzi, Marzia; Comazzi, Stefano

    2018-06-01

    Objectives Flow cytometry (FC) is becoming increasingly popular among veterinary oncologists for the diagnosis of lymphoma or leukaemia. It is accurate, fast and minimally invasive. Several studies of FC have been carried out in canine oncology and applied with great results, whereas there is limited knowledge and use of this technique in feline patients. This is mainly owing to the high prevalence of intra-abdominal lymphomas in this species and the difficulty associated with the diagnostic procedures needed to collect the sample. The purpose of the present study is to investigate whether any pre-analytical factor might affect the quality of suspected feline lymphoma samples for FC analysis. Methods Ninety-seven consecutive samples of suspected feline lymphoma were retrospectively selected from the authors' institution's FC database. The referring veterinarians were contacted and interviewed about several different variables, including signalment, appearance of the lesion, features of the sampling procedure and the experience of veterinarians performing the sampling. Statistical analyses were performed to assess the possible influence of these variables on the cellularity of the samples and the likelihood of it being finally processed for FC. Results Sample cellularity is a major factor in the likelihood of the sample being processed. Moreover, sample cellularity was significantly influenced by the needle size, with 21 G needles providing the highest cellularity. Notably, the sample cellularity and the likelihood of being processed did not vary between peripheral and intra-abdominal lesions. Approximately half of the cats required pharmacological restraint. Side effects were reported in one case only (transient swelling after peripheral lymph node sampling). Conclusions and relevance FC can be safely applied to cases of suspected feline lymphomas, including intra-abdominal lesions. A 21 G needle should be preferred for sampling. This study provides the basis for

  12. Infrared and Raman spectroscopy: principles and spectral interpretation

    National Research Council Canada - National Science Library

    Larkin, Peter

    2011-01-01

    .... The book reviews basic principles, instrumentation, sampling methods, quantitative analysis, origin of group frequencies and qualitative interpretation using generalized Infrared (IR) and Raman spectra...

  13. Principios básicos y alcances metodológicos de las evaluaciones económicas en salud Basic principles and methodological considerations of health economic evaluations

    Directory of Open Access Journals (Sweden)

    Cesar Loza

    2011-09-01

    Full Text Available La economía de la salud es un instrumento indispensable para la gestión sanitaria y las evaluaciones económicas, se pueden considerar como la herramienta que asiste a la toma de decisiones para asignar recursos en el área de la salud. Hoy en día, su uso es creciente en todas las regiones del mundo y fomenta la toma de decisiones basadas en la evidencia, buscando alternativas eficientes y racionales dentro del conjunto de actividades de los servicios. En esta revisión se efecturá una visión general y se delinea los tipos básicos de evaluación económica, sobre todo de las Evaluaciones Económicas (EE completas. Así mismo se revisará los conceptos más relevantes sobre las perspectivas desde las que se pueden realizar las EE, los tipos de costos, el horizonte temporal, los descuentos, la evaluación de la incertidumbre y las reglas de decisión. Finalmente, se describirán conceptos sobre la transferibilidad y la generalización de las Evaluaciones Económicas en salud.Health Economics is an essential instrument for health management, and economic evaluations can be considered as tools assisting the decision-making process for the allocation of resources in health. Currently, economic evaluations are increasingly being used worldwide, thus encouraging evidence-based decision-making and seeking efficient and rational alternatives within the framework of health services activities. In this review, we present an overview and define the basic types of economic evaluations, with emphasis on complete Economic Evaluations (EE. In addition, we review key concepts regarding the perspectives from which EE can be conducted, the types of costs that can be considered, the time horizon, discounting, assessment of uncertainty and decision rules. Finally, we describe concepts about the extrapolation and spread of economic evaluations in health.

  14. XAFS Spectroscopy : Fundamental Principles and Data Analysis

    NARCIS (Netherlands)

    Koningsberger, D.C.; Mojet, B.L.; Dorssen, G.E. van; Ramaker, D.E.

    2000-01-01

    The physical principles of XAFS spectroscopy are given at a sufficiently basic level to enable scientists working in the field of catalysis to critically evaluate articles dealing with XAFS studies on catalytic materials. The described data-analysis methods provide the basic tools for studying the

  15. [Quality Management and Quality Specifications of Laboratory Tests in Clinical Studies--Challenges in Pre-Analytical Processes in Clinical Laboratories].

    Science.gov (United States)

    Ishibashi, Midori

    2015-01-01

    The cost, speed, and quality are the three important factors recently indicated by the Ministry of Health, Labour and Welfare (MHLW) for the purpose of accelerating clinical studies. Based on this background, the importance of laboratory tests is increasing, especially in the evaluation of clinical study participants' entry and safety, and drug efficacy. To assure the quality of laboratory tests, providing high-quality laboratory tests is mandatory. For providing adequate quality assurance in laboratory tests, quality control in the three fields of pre-analytical, analytical, and post-analytical processes is extremely important. There are, however, no detailed written requirements concerning specimen collection, handling, preparation, storage, and shipping. Most laboratory tests for clinical studies are performed onsite in a local laboratory; however, a part of laboratory tests is done in offsite central laboratories after specimen shipping. As factors affecting laboratory tests, individual and inter-individual variations are well-known. Besides these factors, standardizing the factors of specimen collection, handling, preparation, storage, and shipping, may improve and maintain the high quality of clinical studies in general. Furthermore, the analytical method, units, and reference interval are also important factors. It is concluded that, to overcome the problems derived from pre-analytical processes, it is necessary to standardize specimen handling in a broad sense.

  16. The analytic impact of a reduced centrifugation step on chemistry and immunochemistry assays: an evaluation of the Modular Pre-Analytics.

    Science.gov (United States)

    Koenders, Mieke M J F; van Hurne, Marco E J F; Glasmacher-Van Zijl, Monique; van der Linde, Geesje; Westerhuis, Bert W J J M

    2012-09-01

    The COBAS 6000 system can be completed by a Modular Pre-Analytics (MPA), an integrated laboratory automation system that streamlines preanalysis. For an optimal throughput, the MPA centrifuges blood collection tubes for 5 min at 1885 × g - a centrifugation time that is not in concordance with the World Health Organization guidelines which suggest centrifugation for 10/15 min at 2000-3000 × g. In this study, the analytical outcome of 50 serum and 50 plasma samples centrifuged for 5 or 10 min at 1885 × g was investigated. The study included routine chemistry and immunochemistry assays on the COBAS 6000 and the Minicap capillary electrophoresis. Deming-fit and Bland-Altman plots of the 5-min and 10-min centrifugation steps indicated a significant correlation in serum samples. The lipaemia index in plasma samples centrifuged for 5 min displayed a statistically significant variation when compared with the 10-min centrifugation. Preanalytical centrifugation can be successfully down-scaled to a duration of 5 min for most routine chemistry and immunochemistry assays in serum and plasma samples. To prevent inaccurate results in plasma samples with an increased lipaemia index from being reported, the laboratory information system was programmed to withhold results above certain lipaemia indices. The presented data support the use of a 5-min centrifugation step to improve turnaround times, thereby meeting one of the desires of the requesting clinicians.

  17. Pre-Analytical Considerations for Successful Next-Generation Sequencing (NGS: Challenges and Opportunities for Formalin-Fixed and Paraffin-Embedded Tumor Tissue (FFPE Samples

    Directory of Open Access Journals (Sweden)

    Gladys Arreaza

    2016-09-01

    Full Text Available In cancer drug discovery, it is important to investigate the genetic determinants of response or resistance to cancer therapy as well as factors that contribute to adverse events in the course of clinical trials. Despite the emergence of new technologies and the ability to measure more diverse analytes (e.g., circulating tumor cell (CTC, circulating tumor DNA (ctDNA, etc., tumor tissue is still the most common and reliable source for biomarker investigation. Because of its worldwide use and ability to preserve samples for many decades at ambient temperature, formalin-fixed, paraffin-embedded tumor tissue (FFPE is likely to be the preferred choice for tissue preservation in clinical practice for the foreseeable future. Multiple analyses are routinely performed on the same FFPE samples (such as Immunohistochemistry (IHC, in situ hybridization, RNAseq, DNAseq, TILseq, Methyl-Seq, etc.. Thus, specimen prioritization and optimization of the isolation of analytes is critical to ensure successful completion of each assay. FFPE is notorious for producing suboptimal DNA quality and low DNA yield. However, commercial vendors tend to request higher DNA sample mass than what is actually required for downstream assays, which restricts the breadth of biomarker work that can be performed. We evaluated multiple genomics service laboratories to assess the current state of NGS pre-analytical processing of FFPE. Significant differences in pre-analytical capabilities were observed. Key aspects are highlighted and recommendations are made to improve the current practice in translational research.

  18. Attainment of radiation equivalency principle

    International Nuclear Information System (INIS)

    Shmelev, A.N.; Apseh, V.A.

    2004-01-01

    Problems connected with the prospects for long-term development of the nuclear energetics are discussed. Basic principles of the future large-scale nuclear energetics are listed, primary attention is the safety of radioactive waste management of nuclear energetics. The radiation equivalence principle means close of fuel cycle and management of nuclear materials transportation with low losses on spent fuel and waste processing. Two aspects are considered: radiation equivalence in global and local aspects. The necessity of looking for other strategies of fuel cycle management in full-scale nuclear energy on radioactive waste management is supported [ru

  19. Compliance of blood sampling procedures with the CLSI H3-A6 guidelines: An observational study by the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM) working group for the preanalytical phase (WG-PRE)

    NARCIS (Netherlands)

    Simundic, Ana-Maria; Church, Stephen; Cornes, Michael P.; Grankvist, Kjell; Lippi, Giuseppe; Nybo, Mads; Nikolac, Nora; van Dongen-Lases, Edmee; Eker, Pinar; Kovalevskaya, Svjetlana; Kristensen, Gunn B. B.; Sprongl, Ludek; Sumarac, Zorica

    2015-01-01

    Abstract Background: An observational study was conducted in 12 European countries by the European Federation of Clinical Chemistry and Laboratory Medicine Working Group for the Preanalytical Phase (EFLM WG-PRE) to assess the level of compliance with the CLSI H3-A6 guidelines. Methods: A structured

  20. Stem Cell Basics

    Science.gov (United States)

    ... Tips Info Center Research Topics Federal Policy Glossary Stem Cell Information General Information Clinical Trials Funding Information Current ... Basics » Stem Cell Basics I. Back to top Stem Cell Basics I. Introduction: What are stem cells, and ...

  1. Statistical learning methods: Basics, control and performance

    Energy Technology Data Exchange (ETDEWEB)

    Zimmermann, J. [Max-Planck-Institut fuer Physik, Foehringer Ring 6, 80805 Munich (Germany)]. E-mail: zimmerm@mppmu.mpg.de

    2006-04-01

    The basics of statistical learning are reviewed with a special emphasis on general principles and problems for all different types of learning methods. Different aspects of controlling these methods in a physically adequate way will be discussed. All principles and guidelines will be exercised on examples for statistical learning methods in high energy and astrophysics. These examples prove in addition that statistical learning methods very often lead to a remarkable performance gain compared to the competing classical algorithms.

  2. Statistical learning methods: Basics, control and performance

    International Nuclear Information System (INIS)

    Zimmermann, J.

    2006-01-01

    The basics of statistical learning are reviewed with a special emphasis on general principles and problems for all different types of learning methods. Different aspects of controlling these methods in a physically adequate way will be discussed. All principles and guidelines will be exercised on examples for statistical learning methods in high energy and astrophysics. These examples prove in addition that statistical learning methods very often lead to a remarkable performance gain compared to the competing classical algorithms

  3. Principles of asymmetric synthesis

    CERN Document Server

    Gawley, Robert E; Aube, Jeffrey

    2012-01-01

    The world is chiral. Most of the molecules in it are chiral, and asymmetric synthesis is an important means by which enantiopure chiral molecules may be obtained for study and sale. Using examples from the literature of asymmetric synthesis, this book presents a detailed analysis of the factors that govern stereoselectivity in organic reactions. After an explanation of the basic physical-organic principles governing stereoselective reactions, the authors provide a detailed, annotated glossary of stereochemical terms. A chapter on "Practical Aspects of Asymmetric Synthesis" provides a critical overview of the most common methods for the preparation of enantiomerically pure compounds, techniques for analysis of stereoisomers using chromatographic, spectroscopic, and chiroptical methods. The authors then present an overview of the most important methods in contemporary asymmetric synthesis organized by reaction type. Thus, there are four chapters on carbon-carbon bond forming reactions, one chapter on reductions...

  4. Principles of modern physics

    CERN Document Server

    Saxena, A K

    2014-01-01

    Principles of Modern Physics, divided into twenty one chapters, begins with quantum ideas followed by discussions on special relativity, atomic structure, basic quantum mechanics, hydrogen atom (and Schrodinger equation) and periodic table, the three statistical distributions, X-rays, physics of solids, imperfections in crystals, magnetic properties of materials, superconductivity, Zeeman-, Stark- and Paschen Back- effects, Lasers, Nuclear physics (Yukawa's meson theory and various nuclear models), radioactivity and nuclear reactions, nuclear fission, fusion and plasma, particle accelerators and detectors, the universe, Elementary particles (classification, eight fold way and quark model, standard model and fundamental interactions), cosmic rays, deuteron problem in nuclear physics, and cathode ray oscilloscope. NEW TO THE FOURTH EDITION: The CO2 Laser Theory of magnetic moments on the basis of shell model Geological dating Laser Induced fusion and laser fusion reactor. Hawking radiation The cosmological red ...

  5. Use of CTX-I and PINP as bone turnover markers: National Bone Health Alliance recommendations to standardize sample handling and patient preparation to reduce pre-analytical variability.

    Science.gov (United States)

    Szulc, P; Naylor, K; Hoyle, N R; Eastell, R; Leary, E T

    2017-09-01

    The National Bone Health Alliance (NBHA) recommends standardized sample handling and patient preparation for C-terminal telopeptide of type I collagen (CTX-I) and N-terminal propeptide of type I procollagen (PINP) measurements to reduce pre-analytical variability. Controllable and uncontrollable patient-related factors are reviewed to facilitate interpretation and minimize pre-analytical variability. The IOF and the International Federation of Clinical Chemistry (IFCC) Bone Marker Standards Working Group have identified PINP and CTX-I in blood to be the reference markers of bone turnover for the fracture risk prediction and monitoring of osteoporosis treatment. Although used in clinical research for many years, bone turnover markers (BTM) have not been widely adopted in clinical practice primarily due to their poor within-subject and between-lab reproducibility. The NBHA Bone Turnover Marker Project team aim to reduce pre-analytical variability of CTX-I and PINP measurements through standardized sample handling and patient preparation. Recommendations for sample handling and patient preparations were made based on review of available publications and pragmatic considerations to reduce pre-analytical variability. Controllable and un-controllable patient-related factors were reviewed to facilitate interpretation and sample collection. Samples for CTX-I must be collected consistently in the morning hours in the fasted state. EDTA plasma is preferred for CTX-I for its greater sample stability. Sample collection conditions for PINP are less critical as PINP has minimal circadian variability and is not affected by food intake. Sample stability limits should be observed. The uncontrollable aspects (age, sex, pregnancy, immobility, recent fracture, co-morbidities, anti-osteoporotic drugs, other medications) should be considered in BTM interpretation. Adopting standardized sample handling and patient preparation procedures will significantly reduce controllable pre-analytical

  6. Basic operator theory

    CERN Document Server

    Gohberg, Israel

    2001-01-01

    rii application of linear operators on a Hilbert space. We begin with a chapter on the geometry of Hilbert space and then proceed to the spectral theory of compact self adjoint operators; operational calculus is next presented as a nat­ ural outgrowth of the spectral theory. The second part of the text concentrates on Banach spaces and linear operators acting on these spaces. It includes, for example, the three 'basic principles of linear analysis and the Riesz­ Fredholm theory of compact operators. Both parts contain plenty of applications. All chapters deal exclusively with linear problems, except for the last chapter which is an introduction to the theory of nonlinear operators. In addition to the standard topics in functional anal­ ysis, we have presented relatively recent results which appear, for example, in Chapter VII. In general, in writ­ ing this book, the authors were strongly influenced by re­ cent developments in operator theory which affected the choice of topics, proofs and exercises. One ...

  7. Enzymes: principles and biotechnological applications

    Science.gov (United States)

    Robinson, Peter K.

    2015-01-01

    Enzymes are biological catalysts (also known as biocatalysts) that speed up biochemical reactions in living organisms, and which can be extracted from cells and then used to catalyse a wide range of commercially important processes. This chapter covers the basic principles of enzymology, such as classification, structure, kinetics and inhibition, and also provides an overview of industrial applications. In addition, techniques for the purification of enzymes are discussed. PMID:26504249

  8. Analytical and pre-analytical performance characteristics of a novel cartridge-type blood gas analyzer for point-of-care and laboratory testing.

    Science.gov (United States)

    Oyaert, Matthijs; Van Maerken, Tom; Bridts, Silke; Van Loon, Silvi; Laverge, Heleen; Stove, Veronique

    2018-03-01

    Point-of-care blood gas test results may benefit therapeutic decision making by their immediate impact on patient care. We evaluated the (pre-)analytical performance of a novel cartridge-type blood gas analyzer, the GEM Premier 5000 (Werfen), for the determination of pH, partial carbon dioxide pressure (pCO 2 ), partial oxygen pressure (pO 2 ), sodium (Na + ), potassium (K + ), chloride (Cl - ), ionized calcium ( i Ca 2+ ), glucose, lactate, and total hemoglobin (tHb). Total imprecision was estimated according to the CLSI EP5-A2 protocol. The estimated total error was calculated based on the mean of the range claimed by the manufacturer. Based on the CLSI EP9-A2 evaluation protocol, a method comparison with the Siemens RapidPoint 500 and Abbott i-STAT CG8+ was performed. Obtained data were compared against preset quality specifications. Interference of potential pre-analytical confounders on co-oximetry and electrolyte concentrations were studied. The analytical performance was acceptable for all parameters tested. Method comparison demonstrated good agreement to the RapidPoint 500 and i-STAT CG8+, except for some parameters (RapidPoint 500: pCO 2 , K + , lactate and tHb; i-STAT CG8+: pO 2 , Na + , i Ca 2+ and tHb) for which significant differences between analyzers were recorded. No interference of lipemia or methylene blue on CO-oximetry results was found. On the contrary, significant interference for benzalkonium and hemolysis on electrolyte measurements were found, for which the user is notified by an interferent specific flag. Identification of sample errors from pre-analytical sources, such as interferences and automatic corrective actions, along with the analytical performance, ease of use and low maintenance time of the instrument, makes the evaluated instrument a suitable blood gas analyzer for both POCT and laboratory use. Copyright © 2018 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.

  9. Ozone in the atmosphere. Basic principles, natural and human impacts

    Energy Technology Data Exchange (ETDEWEB)

    Fabian, Peter [Technical Univ. Munich (Germany). Immission Research; Dameris, Martin [German Aerospace Center (DLR), Oberpfaffenhofen-Wessling (Germany). Inst. of Atmospheric Physics

    2014-09-01

    Comprehensive coverage of ozone both in the upper and the lower atmosphere. Essential overview of atmospheric ozone research written by two experienced and acknowledged experts. Numerous qualified references to the scientific literature. Peter Fabian and Martin Dameris provide a concise yet comprehensive overview of established scientific knowledge about ozone in the atmosphere. They present both ozone changes and trends in the stratosphere, as well as the effects of overabundance in the troposphere including the phenomenon of photosmog. Aspects such as photochemistry, atmospheric dynamics and global ozone distribution as well as various techniques for ozone measurement are treated. The authors outline the various causes for ozone depletion, the effects of ozone pollution and the relation to climate change. The book provides a handy reference guide for researchers active in atmospheric ozone research and a useful introduction for advanced students specializing in this field. Non-specialists interested in this field will also profit from reading the book. Peter Fabian can look back on a life-long active career in ozone research, having first gained international recognition for his measurements of the global distribution of halogenated hydrocarbons. He also pioneered photosmog investigations in the metropolitan areas of Munich, Berlin, Athens and Santiago de Chile, and his KROFEX facility provided controlled ozone fumigation of adult tree canopies for biologists to investigate the effects of ozone increases on forests. Besides having published a broad range of scientific articles, he has also been the author or editor of numerous books. From 2002 to 2005 he served the European Geosciences Union (EGU) as their first and Founding President. Martin Dameris is a prominent atmospheric modeler whose interests include the impacts of all kinds of natural and man-made disturbances on the atmospheric system. His scientific work focuses on the connections between ozone and climate changes. For many years he has been an active contributor to the WMO scientific ozone depletion assessments, which have been used to monitor the depletion and recovery of the ozone layer in accordance with the Montreal Protocol.

  10. Tips for leaders, part I: basic principles of leadership.

    Science.gov (United States)

    Harolds, Jay A

    2011-08-01

    There are many important leadership skills that can be learned from experience, mentoring, reading, discussions, lectures, etc. The subjects include not only business topics and communication skills but also a variety of tips on what to do and what not to do. These include desirable styles of leadership, how to gain the trust of others in the organization, the significance of the appropriate delegation of tasks, the importance of teams, how to manage change, how to win during a crisis, what to do in the early days of becoming a new leader, preventing the loss of one's practice to a rival organization, improving quality, and others. There are also many important attributes of leaders, including having a strong moral compass. Although each of these topics could be a book in of itself, this short series of articles will briefly examine these issues and others.

  11. Basic principles in the management of thermal injuries

    African Journals Online (AJOL)

    The cervical spine must be protected. Clear the airway by removing foreign material and ensure an open airway. All major burns must receive high flow oxygen for 24 hours. The history of flame burns sustained in an enclosed space and loss of consciousness at the scene are good predictors of potential respiratory injury.

  12. Genomic Signal Processing: Predicting Basic Molecular Biological Principles

    Science.gov (United States)

    Alter, Orly

    2005-03-01

    Advances in high-throughput technologies enable acquisition of different types of molecular biological data, monitoring the flow of biological information as DNA is transcribed to RNA, and RNA is translated to proteins, on a genomic scale. Future discovery in biology and medicine will come from the mathematical modeling of these data, which hold the key to fundamental understanding of life on the molecular level, as well as answers to questions regarding diagnosis, treatment and drug development. Recently we described data-driven models for genome-scale molecular biological data, which use singular value decomposition (SVD) and the comparative generalized SVD (GSVD). Now we describe an integrative data-driven model, which uses pseudoinverse projection (1). We also demonstrate the predictive power of these matrix algebra models (2). The integrative pseudoinverse projection model formulates any number of genome-scale molecular biological data sets in terms of one chosen set of data samples, or of profiles extracted mathematically from data samples, designated the ``basis'' set. The mathematical variables of this integrative model, the pseudoinverse correlation patterns that are uncovered in the data, represent independent processes and corresponding cellular states (such as observed genome-wide effects of known regulators or transcription factors, the biological components of the cellular machinery that generate the genomic signals, and measured samples in which these regulators or transcription factors are over- or underactive). Reconstruction of the data in the basis simulates experimental observation of only the cellular states manifest in the data that correspond to those of the basis. Classification of the data samples according to their reconstruction in the basis, rather than their overall measured profiles, maps the cellular states of the data onto those of the basis, and gives a global picture of the correlations and possibly also causal coordination of these two sets of states. Mapping genome-scale protein binding data using pseudoinverse projection onto patterns of RNA expression data that had been extracted by SVD and GSVD, a novel correlation between DNA replication initiation and RNA transcription during the cell cycle in yeast, that might be due to a previously unknown mechanism of regulation, is predicted. (1) Alter & Golub, Proc. Natl. Acad. Sci. USA 101, 16577 (2004). (2) Alter, Golub, Brown & Botstein, Miami Nat. Biotechnol. Winter Symp. 2004 (www.med.miami.edu/mnbws/alter-.pdf)

  13. Pediatric anesthesia: basic principles, state of the art, future

    National Research Council Canada - National Science Library

    Bissonnette, Bruno; Anderson, Brian J

    2011-01-01

    ... the rights under copyright reserved above, no part of this publication may be reproduced, stored in or introduced into a retrieval system, or transmitted, in any form or by any means (electronic, ...

  14. Basics principles of flat detector computed tomography (FD-CT)

    International Nuclear Information System (INIS)

    Kyriakou, Y.; Struffert, T.; Doerfler, A.; Kalender, W.A.

    2009-01-01

    Flat detectors (FDs) have been developed for use in radiography and fluoroscopy to replace standard X-ray film, film-screen combinations and image intensifiers (II). In comparison to X-ray film and II, FD technology offers higher dynamic range, dose reduction, fast digital readout and the possibility for dynamic acquisitions of image series, yet keeping to a compact design. It appeared logical to employ FD designs also for computed tomography (CT) imaging. FDCT has meanwhile become widely accepted for interventional and intra-operative imaging using C-arm systems. Additionally, the introduction of FD technology was a milestone for soft-tissue CT imaging in the interventional suite which was not possible with II systems in the past. This review focuses on technical and performance issues of FD technology and its wide range of applications for CT imaging. FDCT is not aimed at challenging standard clinical CT as regards to the typical diagnostic examinations, but it has already proven unique for a number of dedicated CT applications offering distinct practical advantages, above all the availability of immediate CT imaging during an intervention. (orig.) [de

  15. Agrobacterium-mediated transformation of plants: Basic principles ...

    African Journals Online (AJOL)

    STORAGESEVER

    2009-10-19

    Oct 19, 2009 ... Furthermore, inactivation of RecQl4A in Arabidopsis leads to 7.5 to 20 fold increase in ... Enriched co-culture medium with 200 mM sucrose and 200 mM ... The effect of temperature during co-culture on T-DNA delivery was first ...

  16. Basic safety principles and practice of WWERs in Hungary

    International Nuclear Information System (INIS)

    Voeroess, L.

    1992-01-01

    The nuclear safety is the actual subject of this presentation and it is considered to be the most important issue, and its permanent improvement is the key responsibility. We share the opinion, that everybody who works in the field of nuclear power generation has to be at such a high level, both in respect of the professional and the moral aspects, which would practically exclude occurrence of accidents causing adverse environmental effects. We are aware that another severe accident occurring in any country of the world would put the whole nuclear industry into a hopeless situation, which - as we have already seen - would seriously influence the Hungarian energy system as well. I try to illustrate in my presentation how can our WWER reactors be evaluated in the highlight of the internationally accepted safety requirements, how safe can they be considered and what can we do in order to ensure at every time the appropriate level of safety. 22 refs, 15 figs

  17. Thymic epithelial tumours: from basic principles to individualised treatment strategies

    Directory of Open Access Journals (Sweden)

    Nicolas Girard

    2013-03-01

    Full Text Available Thymic epithelial tumours represent a wide range of anatomical, clinical, histological and molecular malignant entities that may be aggressive and difficult to treat. The histopathological classification distinguishes thymomas from thymic carcinomas. Thymomas may be associated with autoimmune disorders. The management of thymic epithelial tumours is a paradigm of co-operation between clinicians, surgeons and pathologists, from establishing the diagnosis to organising the multimodal therapeutic strategy. Surgery is the mainstay of the curative-intent treatment, as complete resection represents the most significantly favourable prognostic factor on overall survival. In case of invasion of intra-thoracic structures and/or dissemination to the pleura and the pericardium, precluding complete resection to be achieved, primary chemotherapy has been used to reduce the tumour burden, possibly allowing subsequent surgery and/or radiotherapy. Novel strategies are needed, especially for refractory, recurrent tumours and thymic carcinomas, which carry a poor prognosis. Personalised approaches are currently being developed, as potentially “druggable” molecular targets are emerging from recent integrated genomic analyses. Along with the large variety of questions relative to the treatment strategy, thymic epithelial tumours represent a model of therapeutic implementation and achievement in orphan thoracic oncology, showing how the advent of new results induces new questions, as well as diversifies further clinical research directions and international collaborative initiatives.

  18. Radiofrequency protection guidelines and standards: basic concepts and principles

    International Nuclear Information System (INIS)

    Czerski, P.

    1985-01-01

    Over the past quarter of a century, radiofrequency radiation protection guidelines and standards evolved gradually and are continuously revised and refined. The scientific rationales presented for proposed exposure limits are achieving a considerable scientific sophistication. With increasing scientific validity of the presented arguments, the values of exposure limits are converging and one may hope that they will become convincing and acceptable to all schools of thought. Still more research is needed to refine the available exposure limits. This is recognized by ANSI who revise their recommendations periodically and are now engaged in the preparation of the next revision. INIRC/IRPA is also reconsidering their interim guideline. The Australian Standards Association also stressed the temporary nature of their exposure limits

  19. Ozone in the atmosphere. Basic principles, natural and human impacts

    International Nuclear Information System (INIS)

    Fabian, Peter; Dameris, Martin

    2014-01-01

    Comprehensive coverage of ozone both in the upper and the lower atmosphere. Essential overview of atmospheric ozone research written by two experienced and acknowledged experts. Numerous qualified references to the scientific literature. Peter Fabian and Martin Dameris provide a concise yet comprehensive overview of established scientific knowledge about ozone in the atmosphere. They present both ozone changes and trends in the stratosphere, as well as the effects of overabundance in the troposphere including the phenomenon of photosmog. Aspects such as photochemistry, atmospheric dynamics and global ozone distribution as well as various techniques for ozone measurement are treated. The authors outline the various causes for ozone depletion, the effects of ozone pollution and the relation to climate change. The book provides a handy reference guide for researchers active in atmospheric ozone research and a useful introduction for advanced students specializing in this field. Non-specialists interested in this field will also profit from reading the book. Peter Fabian can look back on a life-long active career in ozone research, having first gained international recognition for his measurements of the global distribution of halogenated hydrocarbons. He also pioneered photosmog investigations in the metropolitan areas of Munich, Berlin, Athens and Santiago de Chile, and his KROFEX facility provided controlled ozone fumigation of adult tree canopies for biologists to investigate the effects of ozone increases on forests. Besides having published a broad range of scientific articles, he has also been the author or editor of numerous books. From 2002 to 2005 he served the European Geosciences Union (EGU) as their first and Founding President. Martin Dameris is a prominent atmospheric modeler whose interests include the impacts of all kinds of natural and man-made disturbances on the atmospheric system. His scientific work focuses on the connections between ozone and climate changes. For many years he has been an active contributor to the WMO scientific ozone depletion assessments, which have been used to monitor the depletion and recovery of the ozone layer in accordance with the Montreal Protocol.

  20. Basic principles of the ECG. The normal ECG

    African Journals Online (AJOL)

    Angel_D

    Southern Sudan Medical Journal vol 3. no 2. 26. How to read an ... Reduce some of the anxiety juniors often experience when faced with an ECG. ... This overall direction of travel of the electrical .... Anne Lancey, Education Centre, St Mary's Hospital, Isle of Wight, UK. .... 'method' section explains how the literature search.

  1. Basic principles and clinical advancements of muscle electrotransfer

    DEFF Research Database (Denmark)

    Hojman, Pernille

    2010-01-01

    clinical potential within DNA vaccination, systemic delivery of therapeutic proteins and correction of gene defects in muscles. In the recent years, DNA electrotransfer to muscle tissue has reached clinical advancement with 8 on-going clinical trials. In the present review, I will draw on the experiences...

  2. Radiology and the internet, basic principles and technical requirements

    International Nuclear Information System (INIS)

    Boegl, K.

    2000-01-01

    As a new communication media the Internet has revolutionized the global exchange of information which is one of the major sources of progress in medicine. This article reviews the history of the Internet's evolution and describes some fundamental technical concepts. The importance of Internet services for everyday professional work is examined with special consideration of radiology. Special emphasis is laid on the current technical limitations that have delayed a broad practical implementation of tele-medical applications. As a highly innovative medical speciality, radiology has always been at the forefront of research and development in the field of clinical Internet applications. By taking a look at recent innovations, the impact of the Internet on the radiologist's working environment of the future is highlighted. (orig.) [de

  3. Conflict at work: basic principles and applied issues

    NARCIS (Netherlands)

    de Dreu, C.K.W.; Zedeck, S.

    2011-01-01

    Conflict at work is quickly classified as a "darkside" construct that has primarily negative, destructive, and aversive consequences to individual employees’ well-being and task performance, to citizenship behavior and performance in work teams, and to organizational fitness and survival chances. In

  4. Radioimmunoassays and 2-site immunoradiometric 'sandwich' assays: basic principles

    Energy Technology Data Exchange (ETDEWEB)

    Rodbard, D

    1988-10-01

    The 'sandwich' or noncompetitive reagent-excess, 2-site immunoradiometric assay (2-site IRMA), ELISA, USERIA, and related techniques, have several advantages compared with the traditional or competitive radioimmunoassays. IRMAs can provide improved sensitivity and specificity. However, IRMAs present some practical problems with nonspecific binding, increased consumption of antibody, biphasic dose response curve, (high dose hook effect), and may require special techniques for dose response curve analysis. We anticipate considerable growth in the popularity and importance of 2-site IRMA.

  5. Images by NMR: basic principles and obtainment technics

    International Nuclear Information System (INIS)

    Tannus, A.

    1987-01-01

    The nuclear magnetic resonance tomographic technique is studied, analysing the nucleus density ρ n(r) of an atomic specie. In the presence of a magnetic field, the nucleus density is expressed in the form of magnetization density M(r). The objective of this paper is to describe the various forms of measures M(r), using the properties of spins systems. (C.G.C.) [pt

  6. Radioimmunoassays and 2-site immunoradiometric "sandwich" assays: basic principles.

    Science.gov (United States)

    Rodbard, D

    1988-10-01

    The "sandwich" or noncompetitive reagent-excess, 2-site immunoradiometric assay (2-site IRMA), ELISA, USERIA, and related techniques, have several advantages compared with the traditional or competitive radioimmunoassays. IRMAs can provide improved sensitivity and specificity. However, IRMAs present some practical problems with nonspecific binding, increased consumption of antibody, biphasic dose response curve, (high dose hook effect), and may require special techniques for dose response curve analysis. We anticipate considerable growth in the popularity and importance of 2-site IRMA.

  7. Photodynamic therapy with 5-aminolevulinic acid: basic principles and applications

    Science.gov (United States)

    Pottier, Roy H.; Kennedy, James C.

    1996-01-01

    Numerous photosensitizing pigments that absorb visible light and are selectively retained in neoplastic tissue are being investigated as potential photochemotherapeutic agents. While much emphasis is being placed on the synthesis of new, far-red absorbing photosensitizers, an alternative approach has been to stimulate the human body to produce its own natural photosensitizer, namely protoporphyrin IX (PpIX). Exogenous 5-aminolevulinic acid (ALA) is rapidly bioconverted into PP by mitochondria, the process being particularly efficient in tumor cells. Since PpIX has a natural and rapid clearing mechanism (via the capture of iron in the process of being converted into heme), ALA-PDT does not suffer from lingering skin phototoxicity. ALA may be introduced orally, intravenously, or topically, and ALA-PDT has been shown to be effective in the treatment of both malignant and non-malignant lesions.

  8. On a new formulation of microphenomena: Basic principles ...

    Indian Academy of Sciences (India)

    edly has great physical and metaphysical consequences which needs ...... includes both particle-picture as well as its associated field-picture. ..... Substantially, one should remember that each μ-particle is also associated with an .... In other words, in all s-states, the electron-field orbits are the same as the classical orbits.

  9. Basic principles set out in the Codex Alimentarius

    International Nuclear Information System (INIS)

    Carrillo, M.

    2013-01-01

    The power point presentation is about food innocuousness, toxicology , nutrition, incertitude, availability data and interpretation, certitude grade, when evaluate the risk, danger identification, exposition population, qualitative probability and estimation, cuantitative risk, exposure limits, methods, methods based on the concept of threshold, general equation

  10. Basic principles in the management of thermal injuries

    African Journals Online (AJOL)

    of necrosis that represents the area of maximum energy transfer and immediate .... An alternative method to use is the patient's .... Initial therapy of minor burns should include administration of ... Vascular access must be adequate and secure.

  11. Basic operation principles of associate co-processor module for ...

    African Journals Online (AJOL)

    With such an organization, it is necessary to read each memory module address and ... for specialized computer systems using a modern element base. ... multiprocessor systems that perform associative functions and data storage functions.

  12. Collaborative Economy: Market Design and Basic Regulatory Principles

    OpenAIRE

    Petropoulos, Georgios

    2017-01-01

    The rise of the collaborative economy platforms reveals that policymakers need to start thinking about how to introduce flexibility in the provision of services in these formal relationships with adequate protection for all of the involved parties.

  13. Basic principles, mosaic of knowledges and adaptive silviculture

    Directory of Open Access Journals (Sweden)

    Borghetti M

    2012-07-01

    Full Text Available Achievements of ecological science and direct experience are of great help for the interpretation of forest ecosystem dynamics and the definition of adaptive procedures for forest manipulation, i.e. adaptive silviculture.

  14. Environmental radiation: basic principles, biological facts, potential risks

    International Nuclear Information System (INIS)

    Rodemann, H.P.

    2000-01-01

    This book describes the complex processes that underlie the effects of different types of radiation at the cellular, organ and organismic level. Technical terms central to the subject matter are printed in italicize and explained in a glossary along with all physical quantities and dimensional units referred to. Through a systematic presentation of various aspects of the effects of environmental radiation on humans the author has endeavoured to make it clear that any discussion on potential health hazards must be conducted specific to the type of radiation in question. Furthermore, to study these issues meaningfully one must have a knowledge of the scientific basis of interactions between the various types of radiation and biological systems and be able to assess the relative impact of environmental radiation compared with other environmental health hazards

  15. Basic principles of magnetic resonance imaging - an update

    International Nuclear Information System (INIS)

    Scherzinger, A.L.; Hendee, W.R.

    1985-01-01

    Magnetic resonance (MR) imaging technology has undergone many technologic advances over the past few years. Many of these advances were stimulated by the wealth of information emerging from nuclear magnetic resonance research in the areas of new and optimal scanning methods and radio-frequency coil design. Other changes arose from the desire to improve image quality, ease siting restrictions and generally facilitate the clinical use of MR equipment. Many questions, however, remain unanswered. Perhaps the most controversial technologic question involves the optimal field strength required for imaging or spectroscopic applications or both. Other issues include safety and clinical efficacy. Technologic issues affect all aspects of MR use including the choice of equipment, examination procedure and image interpretation. Thus, an understanding of recent changes and their theoretic basis is necessary. 92 references, 10 figures, 1 table

  16. Electrochemistry. The basics, with examples

    Energy Technology Data Exchange (ETDEWEB)

    Lefrou, Christine [LEPMI, Saint Martin d' Heres (France); Poignet, Jean-Claude; Fabry, Pierre

    2012-07-01

    This book offers original and new approaches to the teaching of electrochemical concepts, principles and applications. Throughout the text the authors provide a balanced coverage of the thermodynamic and kinetic processes at the heart of electrochemical systems. The first half of the book outlines fundamental concepts appropriate to undergraduate students and the second half gives an in-depth account of electrochemical systems suitable for experienced scientists and course lecturers. Concepts are clearly explained and mathematical treatments are kept to a minimum or reported in appendices. This book features: 1. Questions and answers for self-assessment 2. Basic and advanced level numerical descriptions. 3. Illustrated electrochemistry applications This book is accessible to both novice and experienced electrochemists and supports a deep understanding of the fundamental principles and laws of electrochemistry.

  17. Relleno facial con ácido hialurónico: técnica de pilares y malla de sustentación. Principios básicos para obtener una remodelación facial Facial filling with hyaloronic acid: pillars technique and supporting mesh. Basic principles to obtain a facial remodelling

    Directory of Open Access Journals (Sweden)

    P.J. Erazo

    2009-09-01

    Full Text Available Presentamos dos técnicas de relleno facial utilizando ácido hialurónico y los resultados obtenidos con este método desarrollado por nosotros en base a los principios físicos de sustentación, para optimizar y racionalizar el material de relleno y mejorar los resultados, especialmente la relación costo-beneficio. Hacemos también una presentación general de los principios básicos para conseguir remodelar el tejido cutáneo empleando técnicas de relleno facial. Señalamos la importancia de la evaluación clínica para llegar a un buen diagnóstico, haciendo hincapié en los límites de selección del paciente y enumeramos los puntos importantes y éticos en el uso de materiales de relleno.We present two techniques of facial injectable resorbable filling, using hyaluronic acid (HA, and the different results obtained with both methods, based on physical principles of sustentation, intending to demonstrate how theese techniques can optimize and rationalize the use of the filling substance, contributing to enhance the results and cost-benefit relation. We also make a general presentation of the basic principles that remodel the cutaneous tissue using facial filling methods, as well as the importance of the clinical evaluation to achieve a fine diagnostic, emphasizing the limits when selecting the patient, listing the important and ethical points related to the use of facial filling procedures.

  18. Paediatric airway management: basic aspects

    DEFF Research Database (Denmark)

    Holm-Knudsen, R J; Rasmussen, L S

    2009-01-01

    Paediatric airway management is a great challenge, especially for anaesthesiologists working in departments with a low number of paediatric surgical procedures. The paediatric airway is substantially different from the adult airway and obstruction leads to rapid desaturation in infants and small...... children. This paper aims at providing the non-paediatric anaesthesiologist with a set of safe and simple principles for basic paediatric airway management. In contrast to adults, most children with difficult airways are recognised before induction of anaesthesia but problems may arise in all children...

  19. Magnetic resonance imaging the basics

    CERN Document Server

    Constantinides, Christakis

    2014-01-01

    Magnetic resonance imaging (MRI) is a rapidly developing field in basic applied science and clinical practice. Research efforts in this area have already been recognized with five Nobel prizes awarded to seven Nobel laureates in the past 70 years. Based on courses taught at The Johns Hopkins University, Magnetic Resonance Imaging: The Basics provides a solid introduction to this powerful technology. The book begins with a general description of the phenomenon of magnetic resonance and a brief summary of Fourier transformations in two dimensions. It examines the fundamental principles of physics for nuclear magnetic resonance (NMR) signal formation and image construction and provides a detailed explanation of the mathematical formulation of MRI. Numerous image quantitative indices are discussed, including (among others) signal, noise, signal-to-noise, contrast, and resolution. The second part of the book examines the hardware and electronics of an MRI scanner and the typical measurements and simulations of m...

  20. Health insurance basic actuarial models

    CERN Document Server

    Pitacco, Ermanno

    2014-01-01

    Health Insurance aims at filling a gap in actuarial literature, attempting to solve the frequent misunderstanding in regards to both the purpose and the contents of health insurance products (and ‘protection products’, more generally) on the one hand, and the relevant actuarial structures on the other. In order to cover the basic principles regarding health insurance techniques, the first few chapters in this book are mainly devoted to the need for health insurance and a description of insurance products in this area (sickness insurance, accident insurance, critical illness covers, income protection, long-term care insurance, health-related benefits as riders to life insurance policies). An introduction to general actuarial and risk-management issues follows. Basic actuarial models are presented for sickness insurance and income protection (i.e. disability annuities). Several numerical examples help the reader understand the main features of pricing and reserving in the health insurance area. A short int...

  1. The gauge principle vs. the equivalence principle

    International Nuclear Information System (INIS)

    Gates, S.J. Jr.

    1984-01-01

    Within the context of field theory, it is argued that the role of the equivalence principle may be replaced by the principle of gauge invariance to provide a logical framework for theories of gravitation

  2. Equivalence principles and electromagnetism

    Science.gov (United States)

    Ni, W.-T.

    1977-01-01

    The implications of the weak equivalence principles are investigated in detail for electromagnetic systems in a general framework. In particular, it is shown that the universality of free-fall trajectories (Galileo weak equivalence principle) does not imply the validity of the Einstein equivalence principle. However, the Galileo principle plus the universality of free-fall rotation states does imply the Einstein principle.

  3. SPIDIA-RNA: second external quality assessment for the pre-analytical phase of blood samples used for RNA based analyses.

    Directory of Open Access Journals (Sweden)

    Francesca Malentacchi

    Full Text Available One purpose of the EC funded project, SPIDIA, is to develop evidence-based quality guidelines for the pre-analytical handling of blood samples for RNA molecular testing. To this end, two pan-European External Quality Assessments (EQAs were implemented. Here we report the results of the second SPIDIA-RNA EQA. This second study included modifications in the protocol related to the blood collection process, the shipping conditions and pre-analytical specimen handling for participants. Participating laboratories received two identical proficiency blood specimens collected in tubes with or without an RNA stabilizer. For pre-defined specimen storage times and temperatures, laboratories were asked to perform RNA extraction from whole blood according to their usual procedure and to return extracted RNA to the SPIDIA facility for further analysis. These RNA samples were evaluated for purity, yield, integrity, stability, presence of interfering substances, and gene expression levels for the validated markers of RNA stability: FOS, IL1B, IL8, GAPDH, FOSB and TNFRSF10c. Analysis of the gene expression results of FOS, IL8, FOSB, and TNFRSF10c, however, indicated that the levels of these transcripts were significantly affected by blood collection tube type and storage temperature. These results demonstrated that only blood collection tubes containing a cellular RNA stabilizer allowed reliable gene expression analysis within 48 h from blood collection for all the genes investigated. The results of these two EQAs have been proposed for use in the development of a Technical Specification by the European Committee for Standardization.

  4. The impact of pre-analytical variables on the stability of neurofilament proteins in CSF, determined by a novel validated SinglePlex Luminex assay and ELISA.

    Science.gov (United States)

    Koel-Simmelink, Marleen J A; Vennegoor, Anke; Killestein, Joep; Blankenstein, Marinus A; Norgren, Niklas; Korth, Carsten; Teunissen, Charlotte E

    2014-01-15

    Neurofilament (Nf) proteins have been shown to be promising biomarkers for monitoring and predicting disease progression for various neurological diseases. The aim of this study was to evaluate the effects of pre-analytical variables on the concentration of neurofilament heavy (NfH) and neurofilament light (NfL) proteins. For NfH an in-house newly-developed and validated SinglePlex Luminex assay was used; ELISA was used to analyze NfL. For the NfL ELISA assay, the intra- and inter-assay variation was respectively, 1.5% and 16.7%. Analytical performance of the NfH SinglePlex Luminex assay in terms of sensitivity (6.6pg/mL), recovery in cerebrospinal fluid (CSF) (between 90 and 104%), linearity (from 6.6-1250pg/mL), and inter- and intra-assay variation (<8%) were good. Concentrations of both NfL and NfH appeared not negatively affected by blood contamination, repeated freeze-thaw cycles (up to 4), delayed processing (up to 24hours) and during long-term storage at -20°C, 4°C, and room temperature. A decrease in concentration was observed during storage of both neurofilament proteins up to 21days at 37°C, which was significant by day 5. The newly developed NfH SinglePlex Luminex assay has a good sensitivity and is robust. Moreover, both NfH and NfL are stable under the most prevalent pre-analytical variations. Copyright © 2013 Elsevier B.V. All rights reserved.

  5. Sources of pre-analytical variations in yield of DNA extracted from blood samples: analysis of 50,000 DNA samples in EPIC.

    Directory of Open Access Journals (Sweden)

    Elodie Caboux

    Full Text Available The European Prospective Investigation into Cancer and nutrition (EPIC is a long-term, multi-centric prospective study in Europe investigating the relationships between cancer and nutrition. This study has served as a basis for a number of Genome-Wide Association Studies (GWAS and other types of genetic analyses. Over a period of 5 years, 52,256 EPIC DNA samples have been extracted using an automated DNA extraction platform. Here we have evaluated the pre-analytical factors affecting DNA yield, including anthropometric, epidemiological and technical factors such as center of subject recruitment, age, gender, body-mass index, disease case or control status, tobacco consumption, number of aliquots of buffy coat used for DNA extraction, extraction machine or procedure, DNA quantification method, degree of haemolysis and variations in the timing of sample processing. We show that the largest significant variations in DNA yield were observed with degree of haemolysis and with center of subject recruitment. Age, gender, body-mass index, cancer case or control status and tobacco consumption also significantly impacted DNA yield. Feedback from laboratories which have analyzed DNA with different SNP genotyping technologies demonstrate that the vast majority of samples (approximately 88% performed adequately in different types of assays. To our knowledge this study is the largest to date to evaluate the sources of pre-analytical variations in DNA extracted from peripheral leucocytes. The results provide a strong evidence-based rationale for standardized recommendations on blood collection and processing protocols for large-scale genetic studies.

  6. On the economics of basic liberties

    NARCIS (Netherlands)

    Weikard, H.P.

    2004-01-01

    The paper discusses the problem of initial assignments of rights. An attempt is made to characterise a system of rights in the spirit of Rawls's first principle of justice according to which society should adopt the most extensive system of equal basic liberties.

  7. Virtual Immunology: Software for Teaching Basic Immunology

    Science.gov (United States)

    Berçot, Filipe Faria; Fidalgo-Neto, Antônio Augusto; Lopes, Renato Matos; Faggioni, Thais; Alves, Luiz Anastácio

    2013-01-01

    As immunology continues to evolve, many educational methods have found difficulty in conveying the degree of complexity inherent in its basic principles. Today, the teaching-learning process in such areas has been improved with tools such as educational software. This article introduces "Virtual Immunology," a software program available…

  8. Alfred-Adler's Basic Concepts and Implications.

    Science.gov (United States)

    Lundin, Robert W.

    This book presents the basic principles of Adler's psychology. The first chapter looks at Adlerian psychology as it exists today, and examines earlier influences. The second chapter examines feelings of inferiority and compensation for these feelings. The third chapter considers the nature of goals and how they are formulated. The fourth chapter…

  9. The Net Neutrality Debate: The Basics

    Science.gov (United States)

    Greenfield, Rich

    2006-01-01

    Rich Greenfield examines the basics of today's net neutrality debate that is likely to be an ongoing issue for society. Greenfield states the problems inherent in the definition of "net neutrality" used by Common Cause: "Network neutrality is the principle that Internet users should be able to access any web content they choose and…

  10. Madrid principles of Nagorno-Karabakh settlement

    Directory of Open Access Journals (Sweden)

    K K Babayan

    2014-12-01

    Full Text Available As part of this work presents the analysis of the basic document and the legal framework of the peace settlement of the Nagorno-Karabakh conflict on the modern stage - «Madrid or Basic principles», presented to the sides of the negotiations at the OSCE ministerial conference in the Spanish capital Madrid in November 2007. The both side’s statements, international mediators and institutes form a legal background of the vector and the framework of the negotiation process. There are the fundamental differences and contradictions in the approaches of the sides of the negotiations to the «Basic principles» of the settlement in this article. The work contains a comparison of the various items and elements of the Madrid document both in relation to each other, and to the principles of international law.

  11. Basic Research Firing Facility

    Data.gov (United States)

    Federal Laboratory Consortium — The Basic Research Firing Facility is an indoor ballistic test facility that has recently transitioned from a customer-based facility to a dedicated basic research...

  12. The four variational principles of mechanics

    International Nuclear Information System (INIS)

    Gray, C.G.; Karl, G.; Novikov, V.A.

    1996-01-01

    We argue that there are four basic forms of the variational principles of mechanics: Hamilton close-quote s least action principle (HP), the generalized Maupertuis principle (MP), and their two reciprocal principles, RHP and RMP. This set is invariant under reciprocity and Legendre transformations. One of these forms (HP) is in the literature: only special cases of the other three are known. The generalized MP has a weaker constraint compared to the traditional formulation, only the mean energy bar E is kept fixed between virtual paths. This reformulation of MP alleviates several weaknesses of the old version. The reciprocal Maupertuis principle (RMP) is the classical limit of Schroedinger close-quote s variational principle of quantum mechanics, and this connection emphasizes the importance of the reciprocity transformation for variational principles. Two unconstrained formulations (UHP and UMP) of these four principles are also proposed, with completely specified Lagrange multipliers Percival close-quote s variational principle for invariant tori and variational principles for scattering orbits are derived from the RMP. The RMP is very convenient for approximate variational solutions to problems in mechanics using Ritz type methods Examples are provided. Copyright copyright 1996 Academic Press, Inc

  13. Cost-effective utilisation of basic biochemical laboratory ...

    African Journals Online (AJOL)

    ... of Chemical Pathology, University of KwaZulu-Natal/National Health Laboratory Service (NHLS) ... the basic principles that govern the selection ... issues of quality control and the audit trail of quality assurance in the maintenance of the.

  14. Manual on brachytherapy. Incorporating: Applications guide, procedures guide, basics guide

    International Nuclear Information System (INIS)

    1992-01-01

    In addition to a basic guide to the principles of the production of ionizing radiation and to methods of radiation protection and dosimetry, this booklet includes information about radiation protection procedures for brachytherapy

  15. Label Review Training: Module 1: Label Basics, Page 25

    Science.gov (United States)

    This module of the pesticide label review training provides basic information about pesticides, their labeling and regulation, and the core principles of pesticide label review: clarity, accuracy, consistency with EPA policy, and enforceability.

  16. Label Review Training: Module 1: Label Basics, Page 15

    Science.gov (United States)

    This module of the pesticide label review training provides basic information about pesticides, their labeling and regulation, and the core principles of pesticide label review. Learn about the consequences of improper labeling.

  17. Label Review Training: Module 1: Label Basics, Page 23

    Science.gov (United States)

    This module of the pesticide label review training provides basic information about pesticides, their labeling and regulation, and the core principles of pesticide label review. Lists types of labels that do not require review.

  18. Label Review Training: Module 1: Label Basics, Page 29

    Science.gov (United States)

    This module of the pesticide label review training provides basic information about pesticides, their labeling and regulation, and the core principles of pesticide label review. This page is a quiz on Module 1.

  19. Label Review Training: Module 1: Label Basics, Page 16

    Science.gov (United States)

    This module of the pesticide label review training provides basic information about pesticides, their labeling and regulation, and the core principles of pesticide label review. Learn about the importance of labels and the role in enforcement.

  20. Label Review Training: Module 1: Label Basics, Page 14

    Science.gov (United States)

    This module of the pesticide label review training provides basic information about pesticides, their labeling and regulation, and the core principles of pesticide label review. Learn about positive effects from proper labeling.