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Sample records for baseline hemoglobin level

  1. Current hemoglobin levels are more predictive of disease progression than hemoglobin measured at baseline in patients receiving antiretroviral treatment for HIV type 1 infection

    DEFF Research Database (Denmark)

    Kowalska, Justyna D; Mocroft, Amanda; Blaxhult, Anders;

    2007-01-01

    The role of hemoglobin levels as an independent prognostic marker of progression to AIDS and/or death in HIV-infected patients starting combination antiretroviral therapy (cART) was investigated. A total of 2,579 patients from the EuroSIDA cohort with hemoglobin, CD4 cell count, and HIV RNA viral...

  2. Serum ferritin levels in hemoglobin H disease.

    Science.gov (United States)

    Galanello, R; Melis, M A; Paglietti, E; Cornacchia, G; de Virgiliis, S; Cao, A

    1983-01-01

    This study shows that hemoglobin H disease patients aged between 0.5 and 44 years, usually (27 out of 30) have normal serum ferritin levels according to age. This reconfirms that in this disease there are usually normal iron stores. However, in a few patients (3 out of 30) increased levels were found. This may be due to inappropriate iron medication, transfusions or associated idiopathic hereditary hemocromatosis gene.

  3. Blood hemoglobin level and treatment outcome of early breast cancer

    Energy Technology Data Exchange (ETDEWEB)

    Henke, M.; Sindlinger, F. [Dept. of Radiotherapy, Radiological Univ. Clinic, Freiburg (Germany); Ikenberg, H. [Dept. of Gynecology and Obstretics, Gynecological Univ. Clinic, Freiburg (Germany); Gerds, T.; Schumacher, M. [Inst. for Medical Biometry and Medical Informatics, Univ. Freiburg (Germany)

    2004-01-01

    Background and purpose: to determine whether the blood hemoglobin concentration correlates with the prognosis of patients with early breast cancer and, if so, whether this is restricted to treatment modality. Patients and methods: data were collected retrospectively from patients with early breast cancer (T1,2 NO-2 MO) who underwent either breast-conserving surgery followed by adjuvant radiotherapy (BCS-RT; n = 96) or a modified radical mastectomy (MRM; n = 194). The effect of preoperative blood hemoglobin level, nodal status, histological grading and hormone receptor status on disease-free survival was determined for both treatment modalities using a cox regression model and visualized by kaplan-meier plots. Results: the blood hemoglobin concentration significantly correlated with disease-free survival of patients receiving BCS-RT (relative risk [RR]: 0.67 per g/dl; p = 0.007). This was independent of other known risk factors for breast cancer patients, as determined by multivariate analysis. By contrast, the blood hemoglobin level had no prognostic significance when patients were treated with MRM. Conclusion: blood hemoglobin concentration seems to affect the prognosis of patients with early breast cancer when a treatment schedule that includes radiotherapy is applied. Reduced radiosensitivity due to diminished tumor oxygenation may be the underlying cause. Confirmative trials and studies intended to elucidate the underlying mechanism are warranted. (orig.)

  4. Mercury baseline levels in Flemish soils (Belgium)

    International Nuclear Information System (INIS)

    It is important to establish contaminant levels that are normally present in soils to provide baseline data for pollution studies. Mercury is a toxic element of concern. This study was aimed at assessing baseline mercury levels in soils in Flanders. In a previous study, mercury contents in soils in Oost-Vlaanderen were found to be significantly above levels reported elsewhere. For the current study, observations were extended over two more provinces, West-Vlaanderen and Antwerpen. Ranges of soil Hg contents were distinctly higher in the province Oost-Vlaanderen (interquartile range from 0.09 to 0.43 mg/kg) than in the other provinces (interquartile ranges from 0.7 to 0.13 and 0.7 to 0.15 mg/kg for West-Vlaanderen and Antwerpen, respectively). The standard threshold method was applied to separate soils containing baseline levels of Hg from the data. Baseline concentrations for Hg were characterised by a median of 0.10 mg Hg/kg dry soil, an interquartile range from 0.07 to 0.14 mg/kg and a 90% percentile value of 0.30 mg/kg. The influence of soil properties such as clay and organic carbon contents, and pH on baseline Hg concentrations was not important. Maps of the spatial distribution of Hg levels showed that the province Oost-Vlaanderen exhibited zones with systematically higher Hg soil contents. This may be related to the former presence of many small-scale industries employing mercury in that region. - Increased mercury levels may reflect human activity

  5. Fasting serum glucose and glycosylated hemoglobin level in obesity.

    Science.gov (United States)

    Das, R K; Nessa, A; Hossain, M A; Siddiqui, N I; Hussain, M A

    2014-04-01

    Obesity is a condition in which the body fat stores are increased to an extent which impairs health and leads to serious health consequences. The amount of body fat is difficult to measure directly, and is usually determined from an indirect measure - the body mass index (BMI). Increased BMI in obese persons is directly associated with an increase in metabolic disease, such as type 2 diabetes mellitus. This Analytical cross sectional study was undertaken to assess the relation between obesity and glycemic control of body by measuring fasting serum glucose and glycosylated hemoglobin. This study was carried out in the Department of Physiology, Mymensingh Medical College, Mymensingh from 1st July 2011 to 30th June 2012 on 120 equally divided male and female persons within the age range of 25 to 55 years. Age more than 55 years and less than 25 years and diagnosed case of Hypothyroidism, Cushing's syndrome, polycystic ovary, Antipsychotic drug user and regular steroid users were excluded. Non probability purposive type of sampling technique was used for selecting the study subjects. Measurement of body mass index was done as per procedure. Fasting serum glucose was estimated by glucose oxidase method and Glycosylated hemoglobin by Boronate Affinity method. Statistical analysis was done by SPSS (version 17.0). Data were expressed as Mean±SE and statistical significance of difference among the groups were calculated by unpaired student's 't' test and Pearson's correlation coefficient tests were done as applicable. The Mean±SE of fasting serum glucose was significant at 1% level (P value glycosylated hemoglobin level between control and study groups. But there was positive correlation within each group. Fasting serum glucose also showed a bit stronger positive correlation with BMI. Both obese male and female persons showed higher levels of fasting serum glucose and glycosylated hemoglobin. The observed positive correlation between BMI with fasting serum glucose and

  6. Hemoglobin

    Science.gov (United States)

    ... Low level of iron, folate , vitamin B12 , or vitamin B6 Other chronic illness, such as rheumatoid arthritis HIGHER ... Transfusion reaction - hemolytic Type 1 diabetes Vitamin B12 Vitamin B6 Update Date 2/24/2014 Updated by: Todd ...

  7. Baseline Response Levels Are a Nuisance in Infant Contingency Learning

    Science.gov (United States)

    Millar, W. S.; Weir, Catherine

    2015-01-01

    The impact of differences in level of baseline responding on contingency learning in the first year was examined by considering the response acquisition of infants classified into baseline response quartiles. Whereas the three lower baseline groups showed the predicted increment in responding to a contingency, the highest baseline responders did…

  8. Association of lower hemoglobin level and neuropathology in community-dwelling older persons

    OpenAIRE

    Shah, Raj C.; Schneider, Julie A.; Leurgans, Sue; Bennett, David A.

    2012-01-01

    Lower hemoglobin levels have been associated with cognitive decline in older persons. The objective of this study was to investigate the relationship between lower hemoglobin levels and common, age-related neuropathologies associated with cognitive decline. Hemoglobin and neuropathology measures were available in 113 deceased, community-dwelling, older adults participating in the Rush Memory and Aging Project, a prospective, observational, clinical pathology study of aging. The mean hemoglobi...

  9. The impact of hemoglobin levels on patient and graft survival in renal transplant recipients.

    LENUS (Irish Health Repository)

    Moore, Jason

    2008-08-27

    It remains unclear whether low hemoglobin levels are associated with increased mortality or graft loss after renal transplantation. This study assessed the relationship of hemoglobin levels with patient and graft survival in 3859 patients with functioning renal transplants more than 6-months posttransplantation.

  10. Maternal HIV status affects the infant hemoglobin level

    Science.gov (United States)

    Feleke, Berhanu Elfu

    2016-01-01

    Abstract Children, especially infants, are highly vulnerable to iron-deficiency anemia because of their rapid growth of the brain and the rest of the body. The objectives of this study were to compare the prevalence of iron-deficiency anemia in infants born from HIV-positive mothers and HIV-negative mothers and to identify the determinants of iron-deficiency anemia in infants. A comparative cross-sectional study was conducted in Bahir Dar city. Simple random sampling technique was used to select the study participants. Mothers were interviewed; blood samples were collected from mothers and infants to measure the hemoglobin level and anthropometric indicators were obtained from the infants using world health organization standards. Descriptive statistics were used to estimate the prevalence of infantile anemia. Binary logistic regression and multiple linear regressions were used to identify the determinants of infant anemia. A total of 1459 infants born from HIV-positive and HIV-negative mothers were included. The prevalence of iron-deficiency anemia in infants born from HIV-positive and HIV-negative mothers was 41.9% (95% CI: 39–44). Infantile iron-deficiency anemia was associated with maternal HIV infection (adjusted odds ratio [AOR] 2.54 [95% CI: 1.65–3.9]), stunting (AOR 3.46 [95% CI: 2.41–4.97]), low income (AOR 2.72 [95% CI: 2–3.73]), maternal malaria during pregnancy (AOR 1.81 [95% CI: 1.33–2.47]), use of cow milk before 6 month (AOR 1.82 [95% CI: 1.35–2.45]), residence (AOR 0.09 [95% CI: 0.06–0.13]), history of cough or fever 7 days preceding the survey (AOR 2.71 [95% CI: 1.99–3.69]), maternal hemoglobin (B 0.65 [95% CI: 0.61–0.68]), educational status of mother (B 0.22 [95% CI: 0.2–0.23]), age of the mother (B –0.03 [95% CI: –0.03, –0.02]), and family size (B –0.14 [95% CI: –0.18,–0.11]). PMID:27495044

  11. STUDY OF HEMOGLOBIN LEVELS IN PREGNANT WOMEN AND ITS VARIOUS EFFECTS ON PREGNANCY OUTCOME

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    Maneesha

    2014-03-01

    Full Text Available INTRODUCTION: When maternal anemia is diagnosed before mid-pregnancy, it has been associated with an increased risk of preterm delivery. High maternal hemoglobin is associated with fetal growth restriction. Several epidemiologic studies have shown that high fetal hemoglobin is associated with lower birth weight and increased incidence of IUGR and intrauterine death. MATERIAL AND METHODS: It was a prospective observational study. All pregnant women attending antenatal clinic with first visit before 18 weeks of gestation were included in the study. Hemoglobin levels were done on women at the first visit to antenatal clinic and also in the third trimester between 34-36 weeks. Outcome criteria were birth weight of neonate, gestational age at delivery, maternal weight gain in pregnancy, development of PIH, IUGR, IUD and preterm labor. RESULTS: Increased weight gain in pregnancy was associated with increase in mean birth weight r=0.23 (P value 0.672. Increase in the hemoglobin concentration was associated with increase in birth weight r=0.36 (P value 0.49. It was observed that high hemoglobin concentration in pregnancy was associated with more length of gestation but it was not significant r=0.31 (P value 0.50. CONCLUSION: During early pregnancy normal hemoglobin concentrations were associated with higher birth weights. Weight gain in pregnancy was associated with lower hemoglobin concentrations at term and higher birth weights. The best outcomes of pregnancy were observed in women who had normal hemoglobin concentrations at the onset of pregnancy and who became anemic at term, presumably due to physiologic hemodilution.

  12. Baseline hydrocarbon levels in New Zealand coastal and marine avifauna.

    Science.gov (United States)

    McConnell, H M; Gartrell, B D; Chilvers, B L; Finlayson, S T; Bridgen, P C E; Morgan, K J

    2015-05-15

    The external effects of oil on wildlife can be obvious and acute. Internal effects are more difficult to detect and can occur without any external signs. To quantify internal effects from oil ingestion by wildlife during an oil spill, baseline levels of ubiquitous hydrocarbon fractions, like polycyclic aromatic hydrocarbons (PAHs), need to be established. With these baseline values the extent of impact from exposure during a spill can be determined. This research represents the first investigation of baseline levels for 22 PAHs in New Zealand coastal and marine avian wildlife. Eighty-five liver samples were tested from 18 species. PAHs were identified in 98% of livers sampled with concentrations ranging from 0 to 1341.6 ng/g lipid wt or on wet wt basis, 0 to 29.5 ng/g. Overall, concentrations were low relative to other globally reported avian values. PAH concentration variability was linked with species foraging habitat and migratory patterns. PMID:25707316

  13. Hemoglobin affinity and structure in high-altitude and sea-level carnivores from Peru.

    Science.gov (United States)

    León-Velarde, F; de Muizon, C; Palacios, J A; Clark, D; Monge, C

    1996-04-01

    We compared hemoglobin affinity (P50) and structure of high altitude (HA) carnivores with populations of the same species or genus living at sea level (SL). P50 was measured in cats, pumas and foxes. It differed in animals occupying both niches. SL: cat 29.3 torr, puma 36.3 torr, fox 26.2 torr; HA: cat 22.5 torr, puma 31.1 torr, fox 18.5 torr. Heme and globins were fractionated by HPLC. Puma and fox hemoglobins also showed structural differences. P50 is lower in genotypically HA-adapted species studied and can differentiate SL and HA populations of the same species.

  14. Common Variants at 10 Genomic Loci Influence Hemoglobin A(1C) Levels via Glycemic and Nonglycemic Pathways

    NARCIS (Netherlands)

    Soranzo, Nicole; Sanna, Serena; Wheeler, Eleanor; Gieger, Christian; Radke, Doerte; Dupuis, Josee; Bouatia-Naji, Nabila; Langenberg, Claudia; Prokopenko, Inga; Stolerman, Elliot; Sandhu, Manjinder S.; Heeney, Matthew M.; Devaney, Joseph M.; Reilly, Muredach P.; Ricketts, Sally L.; Stewart, Alexandre F. R.; Voight, Benjamin F.; Willenborg, Christina; Wright, Benjamin; Altshuler, David; Arking, Dan; Balkau, Beverley; Barnes, Daniel; Boerwinkle, Eric; Boehm, Bernhard; Bonnefond, Amelie; Bonnycastle, Lori L.; Boomsma, Dorret I.; Boinstein, Stefan R.; Boettcher, Yvonne; Bumpstead, Suzannah; Burnett-Miller, Mary Susan; Campbell, Harry; Cao, Antonio; Chambers, John; Clark, Robert; Collins, Francis S.; Coresh, Josef; de Geus, Eco J. C.; Dei, Mariano; Deloukas, Panos; Doering, Angela; Egan, Josephine M.; Elosua, Roberto; Ferrucci, Luigi; Forouhi, Nita; Fox, Caroline S.; Franklin, Christopher; Franzosi, Maria Grazia; Gallina, Sophie; Goe, Anuj; Graessler, Juergen; Grallert, Harald; Greinacher, Andreas; Hadley, David; Hall, Alistair; Hamsten, Anders; Hayward, Caroline; Heath, Simon; Herder, Christian; Homuth, Georg; Hottenga, Jouke-Jan; Hunter-Merrill, Rachel; Illig, Thomas; Jackson, Anne U.; Jula, Antti; Kleber, Marcus; Knouff, Christopher W.; Kong, Augustine; Kooner, Jaspal; Koettgen, Anna; Kovacs, Peter; Krohn, Knut; Kuehne, Brigitte; Kuusisto, Johanna; Laakso, Markku; Lathrop, Mark; Lecoeur, Cecile; Li, Man; Li, Mingyao; Loos, Ruth J. F.; Luan, Jian'an; Lyssenko, Valeriya; Maegi, Reedik; Magnusson, Patrik K. E.; Maelarstig, Anders; Mangino, Massimo; Martinez-Larrad, Maria Teresa; Maerz, Winfried; McArdle, Wendy L.; McPherson, Ruth; Meisinger, Christa; Meitinger, Thomas; Melander, Olle; Mohlke, Karen L.; Mooser, Vincent E.; Morken, Mario A.; Narisu, Narisu; Nathan, David M.; Nauck, Matthias; O'Donne, Chris; Oexle, Konrad; Olla, Nazario; Pankow, James S.; Payne, Felicity; Peden, John F.; Pedersen, Nancy L.; Peltonen, Leena; Perola, Markus; Polasek, Ozren; Porcu, Eleonora; Rader, Daniel J.; Rathmann, Wolfgang; Ripatti, Samuli; Rocheleau, Ghislain; Roden, Michael; Rudan, Igor; Salomaa, Veikko; Saxena, Richa; Schlessinger, David; Schunkert, Heribert; Schwarz, Peter; Seedorf, Udo; Selvin, Elizabeth; Serrano-Rios, Manuel; Shrader, Peter; Silveira, Angela; Siscovick, David; Song, Kjioung; Spector, Timothy D.; Stefansson, Kari; Steinthorsdottir, Valgerdur; Strachan, David P.; Strawbridge, Rona; Stumvoll, Michael; Surakka, Ida; Swift, Amy J.; Tanaka, Toshiko; Teumer, Alexander; Thorleifsson, Gudmar; Thorsteinsdottir, Unnur; Toenjes, Anke; Usalai, Gianluca; Vitart, Veronique; Voelzke, Henry; Wallaschofski, Henri; Waterworth, Dawn M.; Watkins, Hugh; Wichmann, H-Erich; Wild, Sarah H.; Willemsen, Gonneke; Williams, Gordon H.; Wilson, James F.; Winkelmann, Juliane; Wright, Alan F.; Zabena, Carina; Zhao, Jing Hua; Epstein, Stephen E.; Erdmann, Jeanette; Hakonarson, Hakon H.; Kathiresan, Sekar; Khaw, Kay-Tee; Roberts, Robert; Samani, Nilesh J.; Fleming, Mark D.; Sladek, Robert; Abecasis, Goncalo; Boehnke, Michael; Froguel, Philippe; Groop, Leif; McCarthy, Mark I.; Kao, W. H. Linda; Florez, Jose C.; Uda, Manuela; Wareham, Nicholas J.; Barroso, Ines; Meigs, James B.; van der Hout, Annemarie

    2010-01-01

    OBJECTIVE-Glycated hemoglobin (HbA(1c)), used to monitor and diagnose diabetes, is influenced by average glycemia over a 2- to 3-month period. Genetic factors affecting expression, turnover, and abnormal glycation of hemoglobin could also be associated with increased levels of HbA(1c). We aimed to i

  15. Self Reported Halitosis in Relation to Glycated Hemoglobin Level in Diabetic Patients

    OpenAIRE

    Al-Zahrani, Mohammad S.; Zawawi, Khalid H; Austah, Obadah N; Al-Ghamdi, Hamed S

    2011-01-01

    Objective: This study was conducted to examine the relationship between the glycated hemoglobin level (HbA1c) and halitosis status among diabetic patients affected with periodontitis and to examine if there is a relationship between halitosis and different periodontal parameters. Methods and Materials: Consecutive type 2 diabetic patients were recruited from patients presented for treatment at a University hospital. Age, gender and smoking were recorded. A structured questionnaire on patients...

  16. Fetal hemoglobin levels are related to metabolic control in diabetic subjects

    Directory of Open Access Journals (Sweden)

    Pardini V.C.

    1999-01-01

    Full Text Available We have investigated the relationship between fetal hemoglobin (HbF levels and metabolic control in subjects with insulin-dependent (N = 79 and non-insulin-dependent diabetes mellitus (N = 242. HbF and hemoglobin A1c (HbA1c levels were increased in subjects with type 1 and type 2 diabetes as compared to levels in nondiabetic individuals (P<0.0001, and were significantly higher in type 1 than in type 2 diabetes subjects. Lower levels of HbA1c and HbF were observed in type 2 diabetes subjects treated by diet, intermediate levels in those treated with oral hypoglycemic agents, and higher levels in those treated with insulin. HbF and HbA1c levels were correlated in type 1 diabetes (R2 = 0.57, P<0.0001 and type 2 diabetes (R2 = 0.58, P<0.0001 subjects. Following intense treatment, twelve diabetic patients showed significant improvement both in HbA1c and HbF values. We conclude that increased HbF levels reflect poor metabolic control in subjects with diabetes mellitus.

  17. Hemoglobin level as a risk factor for lower respiratory tract infections in Lebanese children

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    Sawsan Mourad

    2010-10-01

    Full Text Available Background: Pneumonia is the biggest single cause of childhood death under the age of 5 years, and anemia affects approximately 30% of infants and children all over the world. Aim: Determination of the relationship between anemia and lower respiratory tract infection as a risk factor in Lebanese children. Patients and Methods A total number of two hundred infants and children aged nine months to twelve years were included; One hundred cases were hospitalized for lower respiratory tract infection in Department of Pediatrics, Makassed General Hospital, and one hundred healthy, age and sex matched controls, were selected from outpatient department. Complete blood count, iron level, ferritin level, and total iron binding capacity were taken if hemoglobin level less than eleven gram per deci-liter. In addition peripheral blood smear, chest radiograph and C-reactive protein were done to hospitalized cases. Definition of iron deficiency anemia and normal laboratory values were predetermined. Results: Anemia was found in 32% of hospitalized cases and 16% of healthy controls. Mean hemoglobin level was 9.99 ± 0.62 gram per deci-liter and 11.99 ± 0.92 gram per deci-liter in anemic and non-anemic group respectively with a significant P-value of 0.001. C-reactive protein levels and number hospitalization days were similar among the anemic and non-anemic group. History of recurrent chest infections was significantly higher in both anemic group and hospitalized cases compared to non-anemic group and healthy controls. Low hemoglobin level was a risk factor for lower respiratory tract infection with a P-value of 0.008. Conclusion: Anemic children were two times more susceptible to lower respiratory tract infection compared to the control group, and iron deficiency anemia was predominating. Accurate diagnosis and prevention of anemia, whatever its etiology, is essential.

  18. Hemoglobin level as a risk factor for lower respiratory tract infections in Lebanese children

    Directory of Open Access Journals (Sweden)

    Sawsan Mourad

    2010-01-01

    Full Text Available Background: Pneumonia is the biggest single cause of childhood death under the age of 5 years, and anemia affects approximately 30% of infants and children all over the world. Aim: Determination of the relationship between anemia and lower respiratory tract infection as a risk factor in Lebanese children. Patients and Methods A total number of two hundred infants and children aged nine months to twelve years were included; One hundred cases were hospitalized for lower respiratory tract infection in Department of Pediatrics, Makassed General Hospital, and one hundred healthy, age and sex matched controls, were selected from outpatient department. Complete blood count, iron level, ferritin level, and total iron binding capacity were taken if hemoglobin level less than eleven gram per deci-liter. In addition peripheral blood smear, chest radiograph and C-reactive protein were done to hospitalized cases. Definition of iron deficiency anemia and normal laboratory values were predetermined. Results: Anemia was found in 32% of hospitalized cases and 16% of healthy controls. Mean hemoglobin level was 9.99 ΁ 0.62 gram per deci-liter and 11.99 ΁ 0.92 gram per deci-liter in anemic and non-anemic group respectively with a significant P-value of 0.001. C-reactive protein levels and number hospitalization days were similar among the anemic and non-anemic group. History of recurrent chest infections was significantly higher in both anemic group and hospitalized cases compared to non-anemic group and healthy controls. Low hemoglobin level was a risk factor for lower respiratory tract infection with a P-value of 0.008. Conclusion: Anemic children were two times more susceptible to lower respiratory tract infection compared to the control group, and iron deficiency anemia was predominating. Accurate diagnosis and prevention of anemia, whatever its etiology, is essential.

  19. The Effect of Hemoglobin Levels on Mortality in Pediatric Patients with Severe Traumatic Brain Injury

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    Kevin F. Yee

    2016-01-01

    Full Text Available Objective. There is increasing evidence of adverse outcomes associated with blood transfusions for adult traumatic brain injury patients. However, current evidence suggests that pediatric traumatic brain injury patients may respond to blood transfusions differently on a vascular level. This study examined the influence of blood transfusions and anemia on the outcome of pediatric traumatic brain injury patients. Design. A retrospective cohort analysis of severe pediatric traumatic brain injury (TBI patients was undertaken to investigate the association between blood transfusions and anemia on patient outcomes. Measurements and Main Results. One hundred and twenty patients with severe traumatic brain injury were identified and included in the analysis. The median Glasgow Coma Scale (GCS was 6 and the mean hemoglobin (Hgb on admission was 115.8 g/L. Forty-three percent of patients (43% received at least one blood transfusion and the mean hemoglobin before transfusion was 80.1 g/L. Multivariable regression analysis revealed that anemia and the administration of packed red blood cells were not associated with adverse outcomes. Factors that were significantly associated with mortality were presence of abusive head trauma, increasing PRISM score, and low GCS after admission. Conclusion. In this single centre retrospective cohort study, there was no association found between anemia, blood transfusions, and hospital mortality in a pediatric traumatic brain injury patient population.

  20. Hemoglobin levels and blood pressure are associated in rural black Africans

    DEFF Research Database (Denmark)

    Rasmussen, Jon Bjarke Jarløv; Mwaniki, David L; Kaduka, Lydia U;

    2016-01-01

    OBJECTIVES: The association between blood levels of hemoglobin (B-hgb) and blood pressure (BP) has been widely investigated in Caucasians and Asians but there is a paucity of data in rural black Africans. The objective was to investigate the association between B-hgb and BP in a rural black African...... test were obtained in each participant. RESULTS: Background characteristics among 1,167 participants showed that anemic and non-anemic participants differed significantly from each other as there were more women, lower body mass index and waist circumference (WC), lower degree of hepatic insulin...... resistance and plasma cholesterols among the anemic participants. Furthermore, anemic participants had significantly lower systolic and diastolic BP (P 

  1. Extended-release niacin/laropiprant significantly improves lipid levels in type 2 diabetes mellitus irrespective of baseline glycemic control

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    Bays HE

    2015-02-01

    Full Text Available Harold E Bays,1 Eliot A Brinton,2 Joseph Triscari,3 Erluo Chen,3 Darbie Maccubbin,3 Alexandra A MacLean,3 Kendra L Gibson,3 Rae Ann Ruck,3 Amy O Johnson-Levonas,3 Edward A O’Neill,3 Yale B Mitchel3 1Louisville Metabolic & Atherosclerosis Research Center (L-MARC, Louisville, KY, USA; 2Utah Foundation for Biomedical Research, Salt Lake City, UT, USA; 3Merck & Co, Inc., Whitehouse Station, NJ, USA Background: The degree of glycemic control in patients with type 2 diabetes mellitus (T2DM may alter lipid levels and may alter the efficacy of lipid-modifying agents. Objective: Evaluate the lipid-modifying efficacy of extended-release niacin/laropiprant (ERN/LRPT in subgroups of patients with T2DM with better or poorer glycemic control. Methods: Post hoc analysis of clinical trial data from patients with T2DM who were randomized 4:3 to double-blind ERN/LRPT or placebo (n=796, examining the lipid-modifying effects of ERN/LRPT in patients with glycosylated hemoglobin or fasting plasma glucose levels above and below median baseline levels. Results: At Week 12 of treatment, ERN/LRPT significantly improved low-density lipoprotein cholesterol, high-density lipoprotein cholesterol (HDL-C, non-high-density lipoprotein cholesterol, triglycerides, and lipoprotein (a, compared with placebo, with equal efficacy in patients above or below median baseline glycemic control. Compared with placebo, over 36 weeks of treatment more patients treated with ERN/LRPT had worsening of their diabetes and required intensification of antihyperglycemic medication, irrespective of baseline glycemic control. Incidences of other adverse experiences were generally low in all treatment groups. Conclusion: The lipid-modifying effects of ERN/LRPT are independent of the degree of baseline glycemic control in patients with T2DM (NCT00485758. Keywords: lipid-modifying agents, hyperglycemia, LDL, HDL, triglycerides

  2. Correlation Between the Severity of Diabetic Peripheral Polyneuropathy and Glycosylated Hemoglobin Levels: A Quantitative Study

    Science.gov (United States)

    Lee, Won-Jae; Jang, Sol; Lee, Seung-Hwa

    2016-01-01

    Objective To investigate risk factors for diabetic peripheral polyneuropathy and their correlation with the quantified severity of nerve dysfunction in patients with diabetes mellitus (DM). Methods A total of 187 diabetic patients with clinically suspected polyneuropathy (PN) were subclassified into 2 groups according to electrodiagnostic testing: a DM-PN group of 153 diabetic patients without electrophysiological abnormality and a DM+PN group of 34 diabetic patients with polyneuropathy. For all patients, age, sex, height, weight, duration of DM, and plasma glycosylated hemoglobin (HbA1c) level were comparatively investigated. A composite score was introduced to quantitatively analyze the results of the nerve conduction studies. Logistic regression analysis and multiple regression analysis were used to evaluate correlations between significant risk factors and severity of diabetic polyneuropathy. Results The DM+PN group showed a significantly higher HbA1c level and composite score, as compared with the DM-PN group. Increased HbA1c level and old age were significant predictive factors for polyneuropathy in diabetic patients (odds ratio=5.233 and 4.745, respectively). In the multiple linear regression model, HbA1c and age showed a significant positive association with composite score, in order (β=1.560 and 0.253, respectively). Conclusion Increased HbA1c level indicative of a state of chronic hyperglycemia was a risk factor for polyneuropathy in diabetic patients and a quantitative measure of its severity. PMID:27152276

  3. Evaluation of a New Noninvasive Device in Determining Hemoglobin Levels in Emergency Department Patients

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    Tristan Knutson

    2013-05-01

    Full Text Available Introduction: The Masimo Radical-7 Pulse CO-Oximeter is a medical device recently approved by the US Food and Drug Administration that performs noninvasive oximetry and estimated venous or arterial hemoglobin measurements. A portable, noninvasive device that rapidly measures hemoglobin concentration could be useful in both austere and modern hospital settings. The objective of this study is to determine the degree of variation between the device’s estimated hemoglobin measurement and the actual venous hemoglobin concentration in undifferentiated emergency department (ED patients.Methods: We conducted a prospective, observational, cross-sectional study of adult patients presenting to the ED. The subjects consisted of a convenience sample of adult ED patients who required a complete blood count as part of their care in the ED. A simultaneous probe hemoglobin was obtained and recorded.Results: Bias between probe and laboratory hemoglobin measurements was _0.5 (95% confidence interval,_0.8 to_0.1 but this was not statistically significant from 0 (t 0.05,124¼0.20, P . 0.5. The limits of agreement were _4.7 and 3.8, beyond the clinically relevant standard of equivalency of 6 1 g/dL.Conclusion: These data suggest that noninvasive hemoglobin determination is not sufficiently accurate for emergency department use. [West J Emerg Med. 2013;14(3:283–286.

  4. Genome-wide association analyses based on whole-genome sequencing in Sardinia provide insights into regulation of hemoglobin levels.

    Science.gov (United States)

    Danjou, Fabrice; Zoledziewska, Magdalena; Sidore, Carlo; Steri, Maristella; Busonero, Fabio; Maschio, Andrea; Mulas, Antonella; Perseu, Lucia; Barella, Susanna; Porcu, Eleonora; Pistis, Giorgio; Pitzalis, Maristella; Pala, Mauro; Menzel, Stephan; Metrustry, Sarah; Spector, Timothy D; Leoni, Lidia; Angius, Andrea; Uda, Manuela; Moi, Paolo; Thein, Swee Lay; Galanello, Renzo; Abecasis, Gonçalo R; Schlessinger, David; Sanna, Serena; Cucca, Francesco

    2015-11-01

    We report genome-wide association study results for the levels of A1, A2 and fetal hemoglobins, analyzed for the first time concurrently. Integrating high-density array genotyping and whole-genome sequencing in a large general population cohort from Sardinia, we detected 23 associations at 10 loci. Five signals are due to variants at previously undetected loci: MPHOSPH9, PLTP-PCIF1, ZFPM1 (FOG1), NFIX and CCND3. Among the signals at known loci, ten are new lead variants and four are new independent signals. Half of all variants also showed pleiotropic associations with different hemoglobins, which further corroborated some of the detected associations and identified features of coordinated hemoglobin species production. PMID:26366553

  5. Association of Hemoglobin Concentration With Total and Cause-Specific Mortality in a Cohort of Postmenopausal Women.

    Science.gov (United States)

    Kabat, Geoffrey C; Kim, Mimi Y; Verma, Amit K; Manson, JoAnn E; Lessin, Lawrence S; Kamensky, Victor; Lin, Juan; Wassertheil-Smoller, Sylvia; Rohan, Thomas E

    2016-05-15

    Anemia and low and high levels of hemoglobin have been associated with increased mortality and morbidity. However, most studies have measured hemoglobin at only 1 time point, and few studies have considered possible reverse causation. We used data from the Women's Health Initiative, in which baseline hemoglobin was measured in 160,081 postmenopausal women and year 3 hemoglobin was measured in 75,658 participants, to examine the associations of hemoglobin concentration with total mortality, coronary heart disease mortality, and cancer mortality. Women were enrolled from 1993 to 1998 and followed for a median of 16 years. Cox proportional hazards models were used to estimate the relative mortality hazards associated with deciles of baseline hemoglobin and the mean of baseline + year 3 hemoglobin. Both low and high deciles of baseline hemoglobin were positively associated with all 3 outcomes in the total cohort. In analyses restricted to women with 2 measurements, a low mean hemoglobin level was robustly and positively associated with all 3 outcomes, after exclusion of the early years of follow-up. High mean hemoglobin was also associated with increased risk of total mortality, whereas associations with heart disease mortality and cancer mortality were weaker and inconsistent. Our results provide evidence that low and high levels of hemoglobin are associated with increased risk of mortality in otherwise healthy women. PMID:27076671

  6. Measuring Hemoglobin Levels in the Optic Disc of Parkinson's Disease Patients Using New Colorimetric Analysis Software

    Science.gov (United States)

    Bambo, Maria Pilar; Garcia-Martin, Elena; Satue, Maria; Perez-Olivan, Susana; Alayon, Silvia; Gonzalez-Hernandez, Marta; Polo, Vicente; Larrosa, Jose Manuel; Gonzalez-De la Rosa, Manuel

    2014-01-01

    Objective. To evaluate a new method of measuring hemoglobin (Hb) levels and quantifying the color changes in the optic nerve head of Parkinson's disease (PD) patients. We also compared differences in retinal nerve fiber layer (RNFL) thicknesses obtained using spectral domain optical coherence tomography (OCT) device between PD group and healthy group. Methods. One hundred and fifty-five PD patients and 91 sex- and age-matched healthy subjects were included in this cross-sectional study. OCT examinations and one photograph of the optic disc were performed. The Laguna ONhE (“optic nerve hemoglobin”; Insoft SL, Tenerife, Spain) software was used to analyze the Hb level on the acquired optic disc photographs. Results. PD patients exhibited significantly reduced mean optic disc Hb percentages (57.56% in PD, 67.63% in healthy subjects; P = 0.001) as well as reduced Hb in almost all analyzed sectors, with the largest differences detected in the inferior and nasal sectors. RNFL parameters were significantly reduced in PD patients compared with healthy subjects, especially in the inferior quadrant. Conclusions. Measurements of optic disc Hb levels obtained with the Laguna ONhE software had good ability to detect optic nerve color changes (more papillary paleness and consequently this could suggest optic atrophy and axonal loss) in PD patients. PMID:25587487

  7. National INFOSEC technical baseline: multi-level secure systems

    Energy Technology Data Exchange (ETDEWEB)

    Anderson, J P

    1998-09-28

    The purpose of this report is to provide a baseline description of the state of multilevel processor/processing to the INFOSEC Research Council and at their discretion to the R&D community at large. From the information in the report, it is hoped that the members of the IRC will be aware of gaps in MLS research. A primary purpose is to bring IRC and the research community members up to date on what is happening in the MLS arena. The review will attempt to cover what MLS products are still available, and to identify companies who still offer MLS products. We have also attempted to identify requirements for MLS by interviewing senior officers of the Intelligence community as well as those elements of DoD and DOE who are or may be interested in procuring MLS products for various applications. The balance of the report consists of the following sections; a background review of the highlights of the developments of MLS, a quick summary of where we are today in terms of products, installations, and companies who are still in the business of supplying MLS systems [or who are developing MLS system], the requirements as expressed by senior members of the Intelligence community and DoD and DOE, issues and unmet R&D challenges surrounding MLS, and finally a set of recommended research topics.

  8. Changes of hemoglobin content and glucose levels in the blood of Rattus norvegicus by water extracts of Azadirachta indica

    Institute of Scientific and Technical Information of China (English)

    Shori; Amal Bakr

    2012-01-01

    Presently,there is a growing interest in herbal remedies.Neem (Azadirachta indica) has been used in traditional medicine over centuries.In the present study,the effects of water extracts of Azadirachta indica seeds,stems,flowers and bark on the changes of hemoglobin content (Hb) and glucose levels in the blood of Rattus norvegicus were investigated.Different doses of A.indica water extracts of seeds,stems,flowers and bark were injected to the tested animals every 48 h for 14 days.Significant decrease in both hemoglobin content and glucose levels in the blood samples in all groups of injected rats were compared to control group.However,in all groups higher decrease was shown in the rats injected with 1 g·mL-1 ofA.indica water extracts.In addition,the present study showed no significant relationship between decreased hemoglobin content and glucose levels in blood samples,and increased doses injected.In conclusion,A.indica has the potential to decrease both hemoglobin content and blood glucose levels.

  9. Postpartum hemorrhage is related to the hemoglobin levels at labor: Observational study

    Directory of Open Access Journals (Sweden)

    Kaima A. Frass

    2015-12-01

    Full Text Available Background: Anemia in pregnancy is common and linked to postpartum hemorrhage in terms of uterine atony. The more severe the anemia, the more likely the greater blood loss and adverse outcome. The aim of this study was to examine the association between anemic women at labor and postpartum hemorrhage (PPH during emergency cesarean delivery and to assess the hemoglobin (Hb values at which the emergency hysterectomy is needed. Methods and patients: A cross-sectional study was carried out between (Aug. 1st 2012 and Jul. 30th 2013 at Al Thawra General hospital. Fifty-three cases were included in the study. Results: Postpartum hemorrhage was developed in 53 women (29.1%. Out of 53 women, 21 cases (39.6% had severe uterine atony and required emergency hysterectomy and the remaining 32 cases (60.37% responded to the conservative measures (p 0.03. Most of the hysterectomized women 80.75% (17/21 had Hb levels ⩽ 7 versus 12.5% of the nonhysterectomized patients [OR 29.75; 95% CI 6.564–134.53; p < 0.01]. There was a strong correlation between low Hb levels and blood loss [r = −.619; p < 0.00]. Conclusion: Our study supports the association between anemia (Hb < 10 and the risk of PPH. We also provide evidence of the association between severe anemia and emergency hysterectomy.

  10. S110, a novel decitabine dinucleotide, increases fetal hemoglobin levels in baboons (P. anubis

    Directory of Open Access Journals (Sweden)

    Banzon Virryan

    2010-10-01

    Full Text Available Abstract Background S110 is a novel dinucleoside analog that could have advantages over existing DNA methyltransferase (DNMT inhibitors such as decitabine. A potential therapeutic role for S110 is to increase fetal hemoglobin (HbF levels to treat β-hemoglobinopathies. In these experiments the effect of S110 on HbF levels in baboons and its ability to reduce DNA methylation of the γ-globin gene promoter in vivo were evaluated. Methods The effect of S110 on HbF and γ-globin promoter DNA methylation was examined in cultured human erythroid progenitors and in vivo in the baboon pre-clinical model. S110 pharmacokinetics was also examined in the baboon model. Results S110 increased HbF and reduced DNA methylation of the γ-globin promoter in human erythroid progenitors and in baboons when administered subcutaneously. Pharmacokinetic analysis was consistent with rapid conversion of S110 into the deoxycytosine analog decitabine that binds and depletes DNA. Conclusion S110 is rapidly converted into decitabine, hypomethylates DNA, and induces HbF in cultured human erythroid progenitors and the baboon pre-clinical model.

  11. Maternal HIV status affects the infant hemoglobin level: A comparative cross-sectional study.

    Science.gov (United States)

    Feleke, Berhanu Elfu

    2016-08-01

    Children, especially infants, are highly vulnerable to iron-deficiency anemia because of their rapid growth of the brain and the rest of the body. The objectives of this study were to compare the prevalence of iron-deficiency anemia in infants born from HIV-positive mothers and HIV-negative mothers and to identify the determinants of iron-deficiency anemia in infants.A comparative cross-sectional study was conducted in Bahir Dar city. Simple random sampling technique was used to select the study participants. Mothers were interviewed; blood samples were collected from mothers and infants to measure the hemoglobin level and anthropometric indicators were obtained from the infants using world health organization standards. Descriptive statistics were used to estimate the prevalence of infantile anemia. Binary logistic regression and multiple linear regressions were used to identify the determinants of infant anemia.A total of 1459 infants born from HIV-positive and HIV-negative mothers were included. The prevalence of iron-deficiency anemia in infants born from HIV-positive and HIV-negative mothers was 41.9% (95% CI: 39-44). Infantile iron-deficiency anemia was associated with maternal HIV infection (adjusted odds ratio [AOR] 2.54 [95% CI: 1.65-3.9]), stunting (AOR 3.46 [95% CI: 2.41-4.97]), low income (AOR 2.72 [95% CI: 2-3.73]), maternal malaria during pregnancy (AOR 1.81 [95% CI: 1.33-2.47]), use of cow milk before 6 month (AOR 1.82 [95% CI: 1.35-2.45]), residence (AOR 0.09 [95% CI: 0.06-0.13]), history of cough or fever 7 days preceding the survey (AOR 2.71 [95% CI: 1.99-3.69]), maternal hemoglobin (B 0.65 [95% CI: 0.61-0.68]), educational status of mother (B 0.22 [95% CI: 0.2-0.23]), age of the mother (B -0.03 [95% CI: -0.03, -0.02]), and family size (B -0.14 [95% CI: -0.18,-0.11]). PMID:27495044

  12. Effects of Sleep Disorders on Hemoglobin A1c Levels in Type 2 Diabetic Patients

    Directory of Open Access Journals (Sweden)

    Ahmet Keskin

    2015-01-01

    Full Text Available Background: Studies have reported the presence of sleep disorders in approximately 50-70% of diabetic patients, and these may contribute to poor glycemic control, diabetic neuropathy, and overnight hypoglycemia. The aim of this study was to determine the frequency of sleep disorders in diabetic patients, and to investigate possible relationships between scores of these sleep disorders and obstructive sleep apnea syndrome (OSAS and diabetic parameters (fasting blood glucose, glycated hemoglobin A1c [HbA1c], and lipid levels. Methods: We used the Berlin questionnaire (BQ for OSAS, the Epworth Sleepiness Scale (ESS, and the Pittsburgh Sleep Quality Index (PSQI to determine the frequency of sleep disorders and their possible relationships with fasting blood glucose, HbA1c, and lipid levels. Results: The study included 585 type 2 diabetic patients admitted to family medicine clinics between October and December 2014. Sleep, sleep quality, and sleep scores were used as the dependent variables in the analysis. The ESS scores showed that 54.40% of patients experienced excessive daytime sleepiness, and according to the PSQI, 64.30% experienced poor-quality sleep. The BQ results indicated that 50.20% of patients were at high-risk of OSAS. HbA1c levels correlated significantly with the ESS and PSQI results (r = 0.23, P < 0.001 and r = 0.14, P = 0.001, respectively, and were significantly higher in those with high-risk of OSAS as defined by the BQ (P < 0.001. These results showed that HbA1c levels were related to sleep disorders. Conclusions: Sleep disorders are common in diabetic patients and negatively affect the control of diabetes. Conversely, poor diabetes control is an important factor disturbing sleep quality. Addressing sleep disturbances in patients who have difficulty controlling their blood glucose has dual benefits: Preventing diabetic complications caused by sleep disturbance and improving diabetes control.

  13. Effects of Sleep Disorders on Hemoglobin A1c Levels in Type 2 Diabetic Patients

    Science.gov (United States)

    Keskin, Ahmet; Ünalacak, Murat; Bilge, Uğur; Yildiz, Pinar; Güler, Seda; Selçuk, Engin Burak; Bilgin, Muzaffer

    2015-01-01

    Background: Studies have reported the presence of sleep disorders in approximately 50–70% of diabetic patients, and these may contribute to poor glycemic control, diabetic neuropathy, and overnight hypoglycemia. The aim of this study was to determine the frequency of sleep disorders in diabetic patients, and to investigate possible relationships between scores of these sleep disorders and obstructive sleep apnea syndrome (OSAS) and diabetic parameters (fasting blood glucose, glycated hemoglobin A1c [HbA1c], and lipid levels). Methods: We used the Berlin questionnaire (BQ) for OSAS, the Epworth Sleepiness Scale (ESS), and the Pittsburgh Sleep Quality Index (PSQI) to determine the frequency of sleep disorders and their possible relationships with fasting blood glucose, HbA1c, and lipid levels. Results: The study included 585 type 2 diabetic patients admitted to family medicine clinics between October and December 2014. Sleep, sleep quality, and sleep scores were used as the dependent variables in the analysis. The ESS scores showed that 54.40% of patients experienced excessive daytime sleepiness, and according to the PSQI, 64.30% experienced poor-quality sleep. The BQ results indicated that 50.20% of patients were at high-risk of OSAS. HbA1c levels correlated significantly with the ESS and PSQI results (r = 0.23, P < 0.001 and r = 0.14, P = 0.001, respectively), and were significantly higher in those with high-risk of OSAS as defined by the BQ (P < 0.001). These results showed that HbA1c levels were related to sleep disorders. Conclusions: Sleep disorders are common in diabetic patients and negatively affect the control of diabetes. Conversely, poor diabetes control is an important factor disturbing sleep quality. Addressing sleep disturbances in patients who have difficulty controlling their blood glucose has dual benefits: Preventing diabetic complications caused by sleep disturbance and improving diabetes control. PMID:26668142

  14. Oximetry system performance assessment with POM (acetal) phantoms incorporating hemoglobin calibration standards and customized saturation levels

    Science.gov (United States)

    Jang, Hyounguk; Singh, Karam; Wang, Hsing-Wen; Pfefer, T. J.; Chen, Yu

    2015-03-01

    Standardized approaches for performance assessment of biophotonic devices have the potential to facilitate system development and intercomparison, clinical trial standardization, recalibration, manufacturing quality control and quality assurance during clinical use. Evaluation of devices based on near-infrared spectroscopy (NIRS) for detection of hemoglobin (Hb) content and oxygenation have often involved tissue-simulating phantoms incorporating artificial dyes or flow systems. Towards the development of simple, effective techniques for objective, quantitative evaluation of basic NIRS system performance, we have developed and evaluated two test methods. These methods are based on cuvette inserts in solid turbid phantoms for measuring commercially-available Hb oximetry standards and custom-formulated oxy/deoxy-Hb solutions. Both approaches incorporate solid acetal, or polyoxymethylene (POM), as a tissue-simulating matrix material. First, inverse-adding-doubling (IAD) based on measurements with a spectrophotometer and an integrating sphere was used to measure POM optical properties and their stability over time. Second, two fiberopticprobe- based NIRS systems were used to measure concentration change of oxy- and deoxy-Hb in standard Hb solutions and customized Hb solutions by adding yeast. Differences in system performance were likely due to differences in light source outputs and fiberoptic probe design. Our preliminary results indicate that simple phantom-based approaches based on commercially available polymers and inclusions containing Hb standards, or controlled oxygenation levels may be useful for benchtop assessment of NIRS device quality for a variety of biophotonic devices.

  15. Association of glycosylated hemoglobin level with lipid ratio and individual lipids in type 2 diabetic patients

    Institute of Scientific and Technical Information of China (English)

    Zhe Yan; Yang Liu; Hui Huang

    2012-01-01

    Objective:To study the correlation of lipid ratios and individual lipid indexes of patients with type2 diabetes with glycosylated hemoglobin (HbA1c).Methods:Samples were collected from 128 type2 diabetic patients (aged19-90 years; male 72, female56). The sera were analyzed for HbA1c, total cholesterol (TC), triglycerides (TG), high-density lipoprotein cholesterol (HDL-C) and low-density lipoprotein cholesterol (LDL-C). According to theHbA1c level, the patients were divided into three groups, groupA (HbA1c 10%,n=49). The correlation ofHbA1cwith lipid ratios & individual lipid indexes were analyzed.Results:With the increased level ofHbA1c,LDL-C had a significantly increasing trend (P<0.05); whereasTC went up with the increasedHbA1c, without any significant differences between three groups. There was no significant correlation betweenHbA1c andTG or HDL-C. With the increased level ofHbA1c,TC/HDL-C,LDL-C/HDL-C ratios were gradually increased, with significant differences among groups (P<0.05). The lipid ratios, especiallyLDL-C/HDL-C ratio was more susceptible to impaired lipid metabolism inT2DM patients than individual lipid. Conclusions:LDL-C/HDL-C ratio is helpful in assessing and reducing the risk of cardiovascular disease caused by impaired lipid metabolism in type2 diabetic patients.

  16. Exenatide's effect in reducing weight and glycosylated hemoglobin level in an Arab population with type 2 diabetes

    OpenAIRE

    Bashier, Alaaeldin M.; Abdelgadir, Elamin I.; Khalifa, Azza A.; Rashid, Fouzia; Abuelkheir, Sona M.; Bachet, Fawzi E.

    2014-01-01

    Objectives: To determine whether exenatide is effective in reducing weight and glycosylated hemoglobin level (HbA1c), and to investigate its efficacy in improving lipid profile, blood pressure, and creatinine levels in the Arab population. Methods: This study was conducted at the Endocrine Unit, Dubai Hospital, Dubai, United Arab Emirates. We retrospectively collected data from patients with type 2 diabetes started on exenatide between November 2011 and February 2012. Data included demographi...

  17. Serum Hepcidin Levels and Reticulocyte Hemoglobin Concentrations as Indicators of the Iron Status of Peritoneal Dialysis Patients

    OpenAIRE

    Kosaku Nitta; Shinichiro Oguni; Koji Kataoka; Yukio Hamaguchi; Misao Tsukada; Takahiro Mochizuki; Aya Eguchi; Ken Tsuchiya

    2012-01-01

    Hepcidin is the key mediator of renal anemia, and reliable measurement of serum hepcidin levels has been made possible by the ProteinChip system. We therefore investigated the iron status and serum hepcidin levels of peritoneal dialysis (PD) patients who had not received frequent doses of an erythrocytosis-stimulating agent (ESA) and had not received iron therapy. In addition to the usual iron parameters, the iron status of erythrocytes can be determined by measuring reticulocyte hemoglobin (...

  18. Should we adjust erythropoiesis-stimulating agent dosage to postdialysis hemoglobin levels? A pilot study

    Directory of Open Access Journals (Sweden)

    Castillo Nieves

    2012-07-01

    Full Text Available Abstract Background Predialysis hemoglobin (Hb may overestimate the true erithropoiesis-stimulating agents (ESA requeriments. We tested whether predialysis Hb is a reliable predictor of the postdialysis level to better control ESA dosage, and evaluated the relation between ESA, Hb and cardiovascular events (CVE. Methods Cohort study including 67 stable hemodialysis patients. Pre- and post-dialysis Hb concentrations were measured, and ESA doses were calculated. A model to predict post-dialysis Hb is proposed. During 18 months follow-up, CVE, hospitalizations and mortality were collected. Results After dialysis, Hb cocentration rise by 6.1 ± 5.6%. Using postdialysis Hb, the weight-adjusted ESA dosage would be lower respect to the prescription using predialysis Hb: 104 ± 120 vs 128 ± 124 U/kg/week (P P = 0.001. The prediction model is: Postdialysis Hb (g/dL = 1.636 + 0.871 x predialysis Hb* (g/dL + 0.099 x UF rate** (mL/kg/h - 0.39 for women***. [R2 = 0.74; *P P = 0.001; ***P = 0.03. Conclusions Postdialysis Hb can be a better reflect of the real Hb level in hemodialysis patients. Using postdialysis Hb would avoid the use of inappropriately high ESA doses. The prediction of postdialysis Hb with an adjusted model would help us to identify those patients at risk for ESA overdosification.

  19. Vitamin D deficiency and low hemoglobin level as risk factors for severity of acute lower respiratory tract infections in Egyptian children: A case-control study

    Directory of Open Access Journals (Sweden)

    Abeer S. El Sakka

    2014-03-01

    Conclusion: Vitamin D deficiency was associated with severity of ALRTIs. Low hemoglobin level was more prevalent in those children. Improving the nutritional status in children by preventing vitamin D deficiency and low hemoglobin might influence the outcome of children with ALRTI.

  20. The Relationship between Food Pattern and Hemoglobin Level in Pregnant Women at Maros, South Sulawesi

    Directory of Open Access Journals (Sweden)

    Zulkifli Abdullah

    2011-06-01

    Full Text Available Food consumption pattern is known as a determinant factor for nutritional problems among pregnant mothers. This study was intended to assess food consumption and its relationship to anemia in Maros Districts, Indonesia. This study was conducted in two sub-districts and pregnant mothers was randomly selected (n = 200 and proportionally from both districts. Data was collected by train field workers including measurement of hemoglobin, height and weight, 24-hour recall and food frequency questionnaire. Multivariate analyses were performed to see the relationship between food consumption and anemia. It showed that anemia prevalence was 41% whereas mostly in mild and moderate levels (44% and 55% respectively. The most common pattern of food consumption was rice, fish, and some vegetables. However, vegetables and fruit mostly consumed only 3-6 time a week. Energy and protein intakes were only 59% to 72% recommended dietary allowance (RDA or 1300 kcal and 48 gr respectively. Most vitamin was consumed only around 40% except for vitamin A (76%, 605 RE, folic acid (195%, 1170 ug, and Vitamin B12 (142%, 3,7 ug. However, iron and zinc intakes were only 6.1 gr (17.5% RDA and 5.9 gr (44% RDA, respectively. Multivariate analyses showed that education duration of mothers, nutritional status, iron tablet intakes, vitamin C, and B6 consumption were significantly related to anemia of pregnant mothers in the study and accounted for 24% (p<0.05. We conclude that food consumption was relatively low and caused lack intakes for both macro and micro nutrients of pregnant mothers in the study. Education and nutritional status of the mothers contributed also to the anemia prevalence.

  1. Effects of Sleep Disorders on Hemoglobin A1c Levels in Type 2 Diabetic Patients

    Institute of Scientific and Technical Information of China (English)

    Ahmet Keskin; Murat (ü)nalacak; U(g)ur Bilge; Pinar Yildiz; Seda Güler; Engin Burak Sel(c)uk; Muzaffer Bilgin

    2015-01-01

    Background: Studies have reported the presence of sleep disorders in approximately 50-70% of diabetic patients, and these may contribute to poor glycemic control, diabetic neuropathy, and overnight hypoglycemia.The aim of this study was to determine the frequency of sleep disorders in diabetic patients, and to investigate possible relationships between scores of these sleep disorders and obstructive sleep apnea syndrome (OSAS) and diabetic parameters (fasting blood glucose, glycated hemoglobin A1c [HbA1c], and lipid levels).Methods: We used the Berlin questionnaire (BQ) for OSAS, the Epworth Sleepiness Scale (ESS), and the Pittsburgh Sleep Quality Index (PSQI) to determine the frequency of sleep disorders and their possible relationships with fasting blood glucose, HbA1c, and lipid levels.Results: The study included 585 type 2 diabetic patients admitted to family medicine clinics between October and December 2014.Sleep, sleep quality, and sleep scores were used as the dependent variables in the analysis.The ESS scores showed that 54.40% of patients experienced excessive daytime sleepiness, and according to the PSQI, 64.30% experienced poor-quality sleep.The BQ results indicated that 50.20% of patients were at high-risk of OSAS.HbA 1 c levels correlated significantly with the ESS and PSQI results (r =0.23, P < 0.001 and r =0.14, P =0.001, respectively), and were significantly higher in those with high-risk of OSAS as defined by the BQ (P < 0.001).These results showed that HbA 1 c levels were related to sleep disorders.Conclusions: Sleep disorders are common in diabetic patients and negatively affect the control of diabetes.Conversely, poor diabetes control is an important factor disturbing sleep quality.Addressing sleep disturbances in patients who have difficulty controlling their blood glucose has dual benefits: Preventing diabetic complications caused by sleep disturbance and improving diabetes control.

  2. A retrospective cohort study of blood hemoglobin levels in blood donors and competitive rowers

    DEFF Research Database (Denmark)

    Johansson, P. I.; Ullum, H.; Jensen, Kurt;

    2009-01-01

    .3% of the females demonstrated values above the recommended limit for athletic competition. Thus, the prevalence of a high hemoglobin value was greater in the rowers, of both gender, than in the candidate blood donors (P... people and especially in competition athletes....

  3. Influence of hemoglobin level and dose of administered recombinant human beta erythropoietin on survival of hemodialysis patients

    OpenAIRE

    Knežević Violeta; Milošević Aleksandra; Vodopivec Slavenka; Božić Dušan; Budošan Ivana; Majić Milena

    2011-01-01

    Background/Aim. In patients with end-stage renal disease, treatment with erythropoietin lowers cardiovascular morbidity, improves quality of life and patient survival. The aim of this study was to determine the difference in survival of hemodialysis patients treated with recombinant human beta erythropoietin and patients without this treatment, and to determine the influence of hemoglobin level and erythropoietin dose on the survival of these patients. Method. The study included 291 pat...

  4. [Hospital-acquired anemia and decrease of hemoglobin levels in hospitalized patients].

    Science.gov (United States)

    Gianserra, Carina V; Agüero, Andrés P; Chapelet, Adrián G; Paradiso, Bruno; Spanevello, Valeria A; Del Pino, María A

    2011-01-01

    It is common to observe the development of anemia in hospitalized patients, especially in critical cases. Few studies have evaluated its prevalence and associated factors in patients in the general ward. The purpose of this study is to determine the prevalence, characteristics and associated clinical factors of hospital-acquired anemia and the drop of hemoglobin concentration in hospitalized patients. This is a cross-sectional, prospective and descriptive study. A total of 192 consecutive in-patients in the general ward were studied. Associated risk factors to the drop in hemoglobin by ≥ 2g/dl were analyzed; 139 patients (72.4%) presented anemia; 89 of them (46.4%) had it at admission and 50 (26%) developed hospital-acquired anemia, 47 out of 192 showed a drop in hemoglobin ≥ 2 g/dl(24.48%). They also presented lower values of hematocrite and hemoglobin at discharge (p = 0.01), parenteral hydration at a higher volume (p = 0.01), and lengthier hospitalizations (p = 0.0001). In the univariate analysis, the following variables were statistically significant risk factors: leukocytosis ≥ 11000 mm3 (OR; IC95%: 2,02; 1.03-4; p = 0.01), hospitalization days ≥ 7 (OR; IC95%:3.39; 1.62-7.09; p = 0.0006), parenteral hydration ≥ 1500 ml/day (OR; IC95%: 2.47; 1.06-6.4; p = 0.01), central venous access (OR; IC95%:10.29; 1.75-108.07; p = 0.003) and hospital-acquired anemia (OR; IC95%: 7.06; 3.41-15.83; p = 0.00000004). In the multivariate analysis, the following variables were independent predictive factors of the hemoglobin decrease = 2 g/dl: leukocytosis ≥ 11000 mm3 (OR; IC95%: 2.45; 1.14-5,27; p = 0.02), hospitalization days ≥ 7 (OR; IC95%:5.15; 2.19-12.07; p = 0.0002), parenteral hydration ≥ 1500 ml/day (OR; IC95%: 2.95; 1.13-7.72; p = 0.02), central venous access (OR; IC95%:8.82; 1.37-56.82; p = 0.02). Hospital-acquired anemia has a high prevalence. Lengthier stays, presence of leukocytosis, parenteral hydration and central venous access placement are

  5. Chemoradiation of unresectable pancreatic carcinoma: impact of pretreatment hemoglobin level on patterns of failure

    International Nuclear Information System (INIS)

    Aim: To evaluate, in patients with locally advanced pancreatic carcinoma undergoing concomitant chemoradiation, the impact of pretreatment hemoglobin (Hb) concentration on the outcome in terms of clinical response, local control, metastasis-free survival, disease-free survival, and overall survival. Patients and Methods: 30 patients undergoing concomitant chemoradiation (5-fluorouracil [5-FU], 1,000 mg/m2/day, continuous i.v. infusion days 1-4 of radiotherapy) and external beam radiotherapy (50.4-59.4 Gy) were divided into two groups based on pretreatment median Hb value (11.5 g/dl). The potential prognostic factors examined besides Hb concentration were: tumor site (head vs body-tail), sex (female vs male), cN (cN0 vs cN1), dose of external beam radiotherapy (50.4 Gy vs 59.4 Gy), presence of jaundice at diagnosis (yes vs no), weight loss at diagnosis (≥ 5 kg vs 11.5 g/dl. Metastasis-free survival was 5.1 months in patients with an Hb ≤ 11.5 g/dl and 10.7 months in patients with an Hb > 11.5 g/dl (p = 0.010). Median actuarial disease-free survival was 5.1 and 10.2 months in patients with an Hb ≤ 11.5 and > 11.5 g/dl, respectively (p = 0.026). Median actuarial overall survival was 7.5 and 10.3 months in patients with an Hb ≤ 11.5 and > 11.5 g/dl, respectively (p = 0.039). On multivariate analysis, Hb concentration at diagnosis was the only factor prognostically correlated with metastasis-free survival (p = 0.026), disease-free survival (p = 0.032), and overall survival (p = 0.048). Conclusion: In a group of patients with locally advanced pancreatic carcinoma treated with chemoradiation, a significant correlation was observed between pretreatment Hb levels and metastasis-free survival, disease-free survival, and overall survival. (orig.)

  6. The Relationship between Food Pattern and Hemoglobin Level in Pregnant Women at Maros, South Sulawesi

    OpenAIRE

    Zulkifli Abdullah; Burhanuddin Bahar; Veni Hadju; St Fatimah

    2011-01-01

    Food consumption pattern is known as a determinant factor for nutritional problems among pregnant mothers. This study was intended to assess food consumption and its relationship to anemia in Maros Districts, Indonesia. This study was conducted in two sub-districts and pregnant mothers was randomly selected (n = 200) and proportionally from both districts. Data was collected by train field workers including measurement of hemoglobin, height and weight, 24-hour recall and food frequency quest...

  7. Association between maternal diet factors and hemoglobin levels, glucose tolerance, blood pressure and gestational age in a Hispanic population.

    Science.gov (United States)

    Soto, Roxana; Guilloty, Natacha; Anzalota, Liza; Rosario, Zaira; Cordero, José F; Palacios, Cristina

    2015-06-01

    The aim of this study was to describe the dietary patterns of pregnant women in northern Puerto Rico and explore associations between diet factors with pregnancy related measurements. This analysis is based on the Puerto Rico Testsite for Exploring Contamination Threats (PROTECT), a prospective cohort that is studying environmental risk factors for preterm births in PR. Participants completed a food frequency questionnaire (FFQ) around 20-28 weeks of gestation. The following pregnancy related measures were collected from the medical records: hemoglobin, blood glucose, blood pressure and gestational age. Potential associations between diet factors and pregnancy measures were assessed using chi square analysis with SPSS. A total of 180 participants completed the FFQ; low hemoglobin levels was found in 19.2%, high blood glucose levels was found in 21.1% by fasting blood glucose test and 24.6%by 1-hour 50 g oral glucose screening test, high blood pressure was found in 2.9% (systolic) and 6.5% (diastolic), and pre-term birth was found in 10.4% of the participants. High consumption of rice, desserts and sweets was associated with higher levels of fasting blood glucose levels (p desserts, can lead to high levels of blood glucose and can be a potential predictor of other pregnancy complications during pregnancy in these study participants, such as gestational diabetes. PMID:26817380

  8. Baseline arsenic levels in marine and terrestrial resources from a pristine environment: Isabel Island, Solomon Islands.

    Science.gov (United States)

    Grinham, Alistair; Kvennefors, Charlotte; Fisher, Paul L; Gibbes, Badin; Albert, Simon

    2014-11-15

    Baseline records are crucial in understanding how chemicals of concern impact on the receiving environment. We analysed terrestrial and marine resources from a pristine site on Isabel Island, Solomon Islands, to provide environmental baseline levels for total arsenic and arsenic species composition for commonly consumed marine resources. Our data show that levels of the more toxic inorganic arsenic species were very low or below detectable limits, with the exception of the seaweed Sargassum sp. that contained pentavalent inorganic arsenic levels of 4.63 μg g(-1). Total arsenic concentrations in the majority of marine and terrestrial samples collected were below 2 μg g(-1). The less toxic arsenobetaine was the predominant arsenic species present in all marine fauna samples analysed. This work highlights the need for arsenic speciation analysis to accurately assess potential toxicity of marine resources and provides a crucial baseline to assess the impact of future development within this region. PMID:25199709

  9. Baseline arsenic levels in marine and terrestrial resources from a pristine environment: Isabel Island, Solomon Islands.

    Science.gov (United States)

    Grinham, Alistair; Kvennefors, Charlotte; Fisher, Paul L; Gibbes, Badin; Albert, Simon

    2014-11-15

    Baseline records are crucial in understanding how chemicals of concern impact on the receiving environment. We analysed terrestrial and marine resources from a pristine site on Isabel Island, Solomon Islands, to provide environmental baseline levels for total arsenic and arsenic species composition for commonly consumed marine resources. Our data show that levels of the more toxic inorganic arsenic species were very low or below detectable limits, with the exception of the seaweed Sargassum sp. that contained pentavalent inorganic arsenic levels of 4.63 μg g(-1). Total arsenic concentrations in the majority of marine and terrestrial samples collected were below 2 μg g(-1). The less toxic arsenobetaine was the predominant arsenic species present in all marine fauna samples analysed. This work highlights the need for arsenic speciation analysis to accurately assess potential toxicity of marine resources and provides a crucial baseline to assess the impact of future development within this region.

  10. Night work is associated with glycemic levels and anthropometric alterations preceding diabetes: Baseline results from ELSA-Brasil.

    Science.gov (United States)

    Silva-Costa, Aline; Rotenberg, Lucia; Coeli, Claudia Medina; Nobre, Aline Araújo; Griep, Rosane Härter

    2016-01-01

    Night work has been suggested as a risk factor for diabetes. Individuals with high triglyceride levels, high LDL cholesterol, low HDL cholesterol and obesity, especially abdominal obesity, have a greater chance of developing diabetes. The aim of this study was to analyze glycemic levels, total cholesterol, HDL-C, LDL-C, triglycerides and the anthropometric alterations that precede diabetes, considering their possible association with nigh work among a non-diabetic population. Brazilian Longitudinal Study of Adult Health (ELSA-Brasil) comprises of 15,105 civil servants (35-74 years old) at baseline (2008-2010). The following parameters were analyzed: serum cholesterol (total cholesterol, HDL-C, LDL-C), triglycerides and glucose drawn from 12-hour fasting blood sample, glycated hemoglobin and 2-hour plasma glucose obtained after a 75 g oral glucose tolerance test, BMI, hip and waist measurements using standard equipment and techniques. Participants with diabetes, retired workers and day workers with previous experience of night work were excluded. Generalized linear models, a gamma regression model with an identity link function, were performed to test the association of night work with metabolic and anthropometric variables. The study sample consisted of 3918 men and 4935 women; 305 (7.8%) and 379 (7.7%) of the participants were men and women who worked at night, respectively. Among the men, the exposure to night work was associated with an increase in BMI (b-value = 0.542; p = 0.032) and waist circumference (b-value = 1.66; p = 0.014). For women, increased fasting plasma glucose (b-value = 2.278; p < 0.001), glycated hemoglobin (b-value = 0.099, p < 0.001) and 2 hour plasma glucose (b-value = 5.479, p = 0.001) were associated with night work after adjustments. No significant associations between night work and triglycerides, LDL-C, HDL-C, total cholesterol levels or waist-hip ratio were found. The influences of night work on metabolic and anthropometric factors

  11. Night work is associated with glycemic levels and anthropometric alterations preceding diabetes: Baseline results from ELSA-Brasil.

    Science.gov (United States)

    Silva-Costa, Aline; Rotenberg, Lucia; Coeli, Claudia Medina; Nobre, Aline Araújo; Griep, Rosane Härter

    2016-01-01

    Night work has been suggested as a risk factor for diabetes. Individuals with high triglyceride levels, high LDL cholesterol, low HDL cholesterol and obesity, especially abdominal obesity, have a greater chance of developing diabetes. The aim of this study was to analyze glycemic levels, total cholesterol, HDL-C, LDL-C, triglycerides and the anthropometric alterations that precede diabetes, considering their possible association with nigh work among a non-diabetic population. Brazilian Longitudinal Study of Adult Health (ELSA-Brasil) comprises of 15,105 civil servants (35-74 years old) at baseline (2008-2010). The following parameters were analyzed: serum cholesterol (total cholesterol, HDL-C, LDL-C), triglycerides and glucose drawn from 12-hour fasting blood sample, glycated hemoglobin and 2-hour plasma glucose obtained after a 75 g oral glucose tolerance test, BMI, hip and waist measurements using standard equipment and techniques. Participants with diabetes, retired workers and day workers with previous experience of night work were excluded. Generalized linear models, a gamma regression model with an identity link function, were performed to test the association of night work with metabolic and anthropometric variables. The study sample consisted of 3918 men and 4935 women; 305 (7.8%) and 379 (7.7%) of the participants were men and women who worked at night, respectively. Among the men, the exposure to night work was associated with an increase in BMI (b-value = 0.542; p = 0.032) and waist circumference (b-value = 1.66; p = 0.014). For women, increased fasting plasma glucose (b-value = 2.278; p < 0.001), glycated hemoglobin (b-value = 0.099, p < 0.001) and 2 hour plasma glucose (b-value = 5.479, p = 0.001) were associated with night work after adjustments. No significant associations between night work and triglycerides, LDL-C, HDL-C, total cholesterol levels or waist-hip ratio were found. The influences of night work on metabolic and anthropometric factors

  12. Change in Hemoglobin Levels due to Anesthesia in Mice: An Important Confounder in Studies on Hematopoietic Drugs

    DEFF Research Database (Denmark)

    Gothelf, Anita; Hojman, Pernille; Gehl, Julie

    2009-01-01

    of Hypnorm and Dormicum was 8.7 mmol/L compared to mean hemoglobin 9.9 mmol/L before anesthesia (p important confounder in studies involving measurements of hemoglobin, and this should be taken into account...

  13. Hemoglobin A1c Level Is Not Related to the Severity of Atherosclerosis in Patients with Acute Coronary Syndrome

    Directory of Open Access Journals (Sweden)

    Xinhong Wang

    2015-01-01

    Full Text Available Background. The relationship between hemoglobin A1c (HbA1c levels and the extent of coronary artery stenosis in patients with acute coronary syndrome (ACS remains uncertain. The present study aimed to assess the correlation of HbA1c level with angiographic coronary atherosclerosis. Methods. 292 consecutive ACS patients were enrolled and stratified into three groups according to HbA1c levels (group 1: 40 by logistic regression analysis. Diabetes mellitus (DM and LVEF levels were two independent risk factors for high Gensini score. Conclusions. HbA1c level is not a significant and independent marker for the severity of angiography in ACS patients, even in high-risk patients.

  14. Hemoglobin levels and quality of life in patients with symptomatic chemotherapy-induced anemia: the eAQUA study

    Science.gov (United States)

    Mouysset, Jean-Loup; Freier, Beata; van den Bosch, Joan; Levaché, Charles Briac; Bols, Alain; Tessen, Hans Werner; Belton, Laura; Bohac, G Chet; Terwey, Jan-Henrik; Tonini, Giuseppe

    2016-01-01

    Purpose To assess hemoglobin (Hb) outcomes and fatigue-related quality-of-life (QoL) (electronic assessment) in patients with solid tumors and symptomatic chemotherapy-induced anemia receiving cytotoxic chemotherapy and darbepoetin alfa (DA) or another erythropoiesis-stimulating agent according to European indication. Methods eAQUA was a Phase IV prospective observational study. The primary outcome (assessed in the primary analysis set [PAS]: patients receiving one or more DA dose who had baseline and week 9 assessments for Hb and QoL) was the proportion of patients receiving DA having both Hb increases ≥1 g/dL and improved QoL between baseline and week 9. Functional Assessment of Cancer Therapy-Fatigue (FACT-F) subscale scores were anchored to fatigue visual analog scale scores to determine the minimally important difference for improved QoL. Overall data/data over time are reported for the full analysis set (patients receiving one or more erythropoiesis-stimulating agent dose, n=1,158); week 9 data (ie, data relating to the primary and secondary outcomes) are reported for the PAS (n=510). Baseline and safety data are included for both the full analysis set and PAS. Results In the PAS, 69% of patients had stage IV disease and 96% were fatigued. The minimally important difference in FACT-F change score for QoL improvement was 3.5. From baseline to week 9, 32% (95% confidence interval: 28%–36%) of patients had both improved QoL and an Hb increase ≥1 g/dL; proportions were similar across the most common tumor types. At week 9, 49% and 58% of patients had improved QoL or Hb increases ≥1 g/dL, respectively; 70% and 76% had QoL or Hb improvements between baseline and study end, respectively. In the PAS, 16% of patients required transfusions and 32% required iron supplementation. Few patients (<1%) reported adverse drug reactions. Conclusion In this study, patients with solid tumors receiving DA per European indication for symptomatic chemotherapy-induced anemia

  15. Intensive management program to improve glycosylated hemoglobin levels and adherence to diet in patients with type 2 diabetes.

    Science.gov (United States)

    Song, Min-Sun; Kim, Hee-Seung

    2009-02-01

    This study investigated the effects of a diabetes outpatient intensive management program (DOIMP) on glycosylated hemoglobin (HbA(1)c) levels and adherence to diabetes control recommendations over a 12-week follow-up period for patients with diabetes. The DOIMP was composed of multidisciplinary diabetes education, complication monitoring, and telephone counseling. Twenty-five patients in the intervention group participated in the DOIMP, whereas 24 in the control group were briefed on the conventional description of diabetes mellitus by diabetes education nurses. Patients in the intervention group decreased their mean HbA(1)c levels by 2.3%, as compared with 0.4% in the control group. There was a significant increase in adherence to diet for the intervention group as compared with the control group. These findings indicate that the DOIMP can improve HbA(1)c levels and adherence to diet in patients with type 2 diabetes.

  16. Positive Effect of Large Birth Intervals on Early Childhood Hemoglobin Levels in Africa Is Limited to Girls: Cross-Sectional DHS Study.

    Directory of Open Access Journals (Sweden)

    Robel Afeworki

    Full Text Available Short birth intervals are independently associated with increased risk of adverse maternal, perinatal, infant and child outcomes. Anemia in children, which is highly prevalent in Africa, is associated with an increased risk of morbidity and mortality. Birth spacing is advocated as a tool to reduce anemia in preschool African children, but the role of gender differences and contextual factors has been neglected. The present study aims to determine to what extent the length of preceding birth interval influences the hemoglobin levels of African preschool children in general, as well as for boys and girls separately, and which contextual factors thereby play a crucial role.This cross-sectional study uses data from Demographic and Health Surveys (DHS conducted between 2003 and 2011 in 20 African countries. All preschool children aged 6-59 months with a valid hemoglobin measurement and a preceding birth interval of 7-72 months as well as their corresponding multigravida mothers aged 21-49 years were included in the study. Hemoglobin levels of children and mothers were measured in g/l, while birth intervals were calculated as months difference between consecutive births. Multivariate analyses were done to examine the relationship between length of preceding birth interval and child hemoglobin levels, adjusted for factors at the individual, household, community, district, and country level. A positive linear relationship was observed between birth interval and the 49,260 included children's hemoglobin level, whereby age and sex of the child, hemoglobin level of the mother, household wealth, mother's education and urbanization of place of residence also showed positive associations. In the interaction models, the effect of a month increase in birth interval is associated with an average increase of 0.025 g/l in hemoglobin level (P = 0.001 in girls, while for boys the effect was not significant. In addition, for girls, the effect of length of preceding

  17. Serum Hepcidin Levels and Reticulocyte Hemoglobin Concentrations as Indicators of the Iron Status of Peritoneal Dialysis Patients

    Directory of Open Access Journals (Sweden)

    Aya Eguchi

    2012-01-01

    Full Text Available Hepcidin is the key mediator of renal anemia, and reliable measurement of serum hepcidin levels has been made possible by the ProteinChip system. We therefore investigated the iron status and serum hepcidin levels of peritoneal dialysis (PD patients who had not received frequent doses of an erythrocytosis-stimulating agent (ESA and had not received iron therapy. In addition to the usual iron parameters, the iron status of erythrocytes can be determined by measuring reticulocyte hemoglobin (RET-He. The mean serum hepcidin level of the PD patients (n=52 was 80.7 ng/mL. Their serum hepcidin levels were significantly positively correlated with their serum ferritin levels and transferrin saturation (TSAT levels, but no correlations were found between their serum hepcidin levels and RET-He levels, thereby suggesting that hepcidin has no effect on the iron dynamics of reticulocytes. Since low serum levels of CRP and IL-6, biomarkers of inflammation, were not correlated with the serum hepcidin levels, there is likely to be a threshold for induction of hepcidin expression by inflammation.

  18. 慢性肾脏病不同分期血红蛋白水平的变化及其影响因素分析%Cross-sectional Study of Hemoglobin Level in Patients with Chronic Kidney Disease

    Institute of Scientific and Technical Information of China (English)

    刘迅; 吕凛生; 王成; 彭晖; 石成钢; 陈珠江; 娄探奇

    2011-01-01

    目的 探讨慢性肾脏病(CKD)患者血红蛋白水平的基础状况.方法 对中山大学附属第三医院肾内科门诊就诊的CKD患者进行为期9个月的前瞻性横断面调查.结果 共有655例CKD病例入选.前四位慢性肾脏病的病因分别为原发性肾小球疾病、狼疮肾炎、高血压肾病和糖尿病肾病.CKD3期以后,患者血红蛋白水平逐级降低,贫血发生率逐级上升,差异均有显著性(P<0.01).多元回归分析结果显示,血红蛋白水平与肾小球滤过率、体质指数、血白蛋白水平、原发性肾小球疾病呈显著性正相关(P<0.01),与收缩压、年龄呈显著性负相关(P<0.05).结论 血红蛋白水平是慢性肾脏病患者CKD3期以后肾脏病情动态变化的重要指标.%Objective To present the baseline characteristics of hemoglobin level in patients with chronic kidney disease (CKD). Methods A cross-sectional study was carried out Results 655 outpatients were enrolled in a period of 9 months study. The top four cause of CKD in these patients were primary glomerular disease, lupus nephritis,essential hypertension and diabetic nephropathy. In patients with GFR<60 ml/min, hemoglobin level and the incidence of anemia were associated with the stage of CKD. A significantly positive correlation of hemoglobin level was found with glomerular filtration rate, BMI, chronic glomerulone-phritis as well as serum albumin level. A significantly negative correlation was discovered between hemoglobin level and the level of systolic blood pressure and age. Conclusion Hemoglobin level is an important indicator for the dynamic change of the CKD with GFR<60 ml/min.

  19. Trends in postoperative infection rates and their relationship to glycosylated hemoglobin levels in diabetic patients undergoing foot and ankle surgery.

    Science.gov (United States)

    Jupiter, Daniel C; Humphers, Jon M; Shibuya, Naohiro

    2014-01-01

    The association of hyperglycemia with postoperative infectious complications after foot and ankle surgery has been well studied. However, many surgeons in their current practice use the somewhat arbitrary cutoff of 7% glycosylated hemoglobin (HbA1c) as the level above which surgery is considered unsafe and conducive to complications. Our goal in the present study was to assess the relationship between the HbA1c levels and the rate of postoperative infection to begin to determine whether 7% is a suitable cutoff or whether this level needs to be reevaluated. Furthermore, we were interested in the general trends relating to the infection rates and preoperative HbA1c levels. Our preliminary, subjective, analysis has indicated that infection rates increase steadily as the HbA1c increases toward 7.3%, increase rapidly at an HbA1c of 7.3% to 9.8%, and then level off. Additional study is warranted to better understand the role played by other covariates in determining the infection rate and to investigate whether patient selection has influenced the appearance of decreased infection rates at high HbA1c levels. Additional study could also assess similar relationships for other types of complication, such as nonunion, and perhaps examine different foot and ankle procedures in isolation.

  20. Hemoglobin levels and quality of life in patients with symptomatic chemotherapy-induced anemia: the eAQUA study

    Directory of Open Access Journals (Sweden)

    Mouysset JL

    2016-01-01

    Full Text Available Jean-Loup Mouysset,1 Beata Freier,2 Joan van den Bosch,3 Charles Briac Levaché,4 Alain Bols,5 Hans Werner Tessen,6 Laura Belton,7 G Chet Bohac,8 Jan-Henrik Terwey,9 Giuseppe Tonini101Department of Medical Oncology, Clinique Rambot Provencale, Aix en Provence, France; 2Clinical Oncology, Wojewodzki Szpital Specjalistyczny, Wroclaw, Poland; 3Department of Internal Medicine/Oncology, Albert Schweitzer Ziekenhuis locatie Dordwijk, Dordrecht, the Netherlands; 4Radiotherapy Service, Medical Oncology, Polyclinique Francheville, Périgueux, France; 5Central Pharmacy, AZ Sint-Jan Brugge-Oostende AV, Brugge, Belgium; 6Private Oncology Practice. Goslar, Germany; 7LB Biostatistics, London, UK; 8Clinical Research, Amgen Inc., Thousand Oaks, CA, USA; 9Medical Development – Oncology, Amgen (Europe GmbH, Zug, Switzerland; 10Department of Medical Oncology, Università Campus Bio-Medico, Roma, ItalyPurpose: To assess hemoglobin (Hb outcomes and fatigue-related quality-of-life (QoL (electronic assessment in patients with solid tumors and symptomatic chemotherapy-induced anemia receiving cytotoxic chemotherapy and darbepoetin alfa (DA or another erythropoiesis-stimulating agent according to European indication.Methods: eAQUA was a Phase IV prospective observational study. The primary outcome (assessed in the primary analysis set [PAS]: patients receiving one or more DA dose who had baseline and week 9 assessments for Hb and QoL was the proportion of patients receiving DA having both Hb increases ≥1 g/dL and improved QoL between baseline and week 9. Functional Assessment of Cancer Therapy-Fatigue (FACT-F subscale scores were anchored to fatigue visual analog scale scores to determine the minimally important difference for improved QoL. Overall data/data over time are reported for the full analysis set (patients receiving one or more erythropoiesis-stimulating agent dose, n=1,158; week 9 data (ie, data relating to the primary and secondary outcomes are reported

  1. Level 3 Baseline Risk Assessment for Building 3515 at Oak Ridge National Lab., Oak Ridge, TN

    Energy Technology Data Exchange (ETDEWEB)

    Wollert, D.A.; Cretella, F.M.; Golden, K.M. [Univ. of Tennessee, Knoxville, TN (United States)] [and others

    1995-08-01

    The baseline risk assessment for the Fission Product Pilot Plant (Building 3515) at the Oak Ridge National laboratory (ORNL) provides the Decontamination and Decommissioning (D&D) Program at ORNL and Building 3515 project managers with information concerning the results of the Level 3 baseline risk assessment performed for this building. The document was prepared under Work Breakdown Structure 1.4.12.6.2.01 (Activity Data Sheet 3701, Facilities D&D) and includes information on the potential long-term impacts to human health and the environment if no action is taken to remediate Building 3515. Information provided in this document forms the basis for the development of remedial alternatives and the no-action risk portion of the Engineering Evaluation/Cost Analysis report.

  2. Association between Hemoglobin Levels in the First 3 Days of Life and Bronchopulmonary Dysplasia in Preterm Infants.

    Science.gov (United States)

    Duan, Jun; Kong, Xiangyong; Li, Qiuping; Hua, Shaodong; Zhang, Sheng; Feng, Zhichun; Zhang, Xiaoying

    2016-08-01

    Objective The objective of this study was to determine the association between hemoglobin (Hb) levels in the first 3 days of life and bronchopulmonary dysplasia (BPD) in preterm infants. Study Design The study population comprises 147 neonates with a gestational age (GA) of less than 32 weeks who were admitted to BaYi Children's Hospital Affiliated to Beijing Military General Hospital from January 2014 to May 2015. Hb levels in the first 3 days of life, maternal and infant characteristics, were recorded and then analyzed. Results BPD patients had a lower GA and birth weight than non-BPD patients. Rates of surfactant use, use of early inhalation hormone, days of mechanical ventilation > 2 weeks, and patent ductus arteriosus in BPD patients were higher and have a significant difference. Number of transfusions was higher in BPD patients. Lower Hb levels in the first 3 days of life were also observed in BPD patients. A cutoff value of Hb levels was determined as 155.5 g/L. Hb ≤ 155 g/L in the first 3 days of life was a significant risk factor for BPD. Conclusion Our study demonstrated that lower Hb levels in the first 3 days of life may increase the risk of developing BPD in preterm infants. PMID:27120476

  3. Relationship status and relationship instability, but not dominance, predict individual differences in baseline cortisol levels.

    Directory of Open Access Journals (Sweden)

    Dario Maestripieri

    Full Text Available We investigated variation in baseline cortisol levels in relation to relationship status (single or in a relationship, relationship characteristics (length, stability, presence or absence of clear dominance, or individual attributes (dominant or subordinate status, relative physical attractiveness, relationship worries. Study participants were 77 men and 75 women aged between 18 and 38 years. Individuals in romantic relationships had lower cortisol levels than singles. Individuals of African ethnicity, however, showed the opposite pattern. Individuals who perceived their relationship to be highly unstable had higher cortisol levels. Aside from African-Americans, married individuals reported the lowest relationship instability and the lowest cortisol levels, followed by individuals in long-term relationships, and by individuals in short-term relationships. The presence or absence of clear dominance in the relationship, dominance status, or relationship worries did not affect cortisol levels. Therefore relationship status and relationship instability were better predictors of variation in cortisol (presumably through stress-related mechanisms than individual attributes.

  4. Aerobic bacterial microbiota of the conjunctiva in diabetic patients with normal and altered glycated hemoglobin levels in two regions in Brazil

    Directory of Open Access Journals (Sweden)

    Natalia Pimentel Moreno

    2014-12-01

    Full Text Available Purpose: To study the aerobic bacterial microbiota of the conjunctiva in diabetic patients with regard to the management of diabetes, assessed using glycated hemoglobin levels. Methods: A cross-sectional study was conducted using conjunctival smears of diabetic patients from both sexes and with different ages, residing in two different Brazilian cities (Sorocaba and Rio Branco. A control group of non-diabetic patients was also included. The diabetic patients were considered to have controlled diabetes when their glycated hemoglobin level was ≤7% and blood glucose level was ≤126 mg/dL. Patients with non-controlled diabetes were those with glycated hemoglobin levels >7% and blood glucose levels >126 mg/dL. The samples obtained were inoculated in Brain-Heart Infusion broth and in culture media for aerobic bacteria (blood and chocolate agars; bacterial growth was evaluated in a microbiology laboratory. Results: A total of 120 eyes of 120 patients were included in the present study. The percentage of cultures in which bacterial growth was observed was greater in diabetic patients, although the difference was not statistically significant (p=0.103. There was a greater trend toward bacterial growth in the conjunctiva of diabetic patients with altered fasting blood glucose. There was no difference in the frequency of bacterial growth on the conjunctiva between diabetic patients with normal or altered glycated hemoglobin levels. In Sorocaba, conjunctival bacterial growth was similar to that observed in Rio Branco. The microorganism most frequently detected in the present study was Staphylococcus epidermidis, followed by Staphylococcus aureus, Proteus mirabilis, and Escherichia coli. Conclusion: There was no difference between diabetic patients with normal or altered glycated hemoglobin levels. The microorganisms found were similar to those found in studies investigating the conjunctival bacterial flora of diabetic and non-diabetic patients.

  5. Comparison of Frequency of Periprocedural Myocardial Infarction in Patients With and Without Diabetes Mellitus to Those With Previously Unknown but Elevated Glycated Hemoglobin Levels (from the TWENTE Trial)

    NARCIS (Netherlands)

    Tandjung, K.; Houwelingen, van K.G.; Jansen, H.; Basalus, M.W.; Sen, H.; Löwik, M.M.; Stoel, M.G.; Louwerenburg, J.H.; Man, de F.H.; Linssen, G.C.; Nijhuis, R.; Nienhuis, M.B.; Palen, van der J.A.M.; Stolk, R.P.; Birgelen, von C.

    2012-01-01

    In patients without a history of diabetes mellitus, increased levels of glycated hemoglobin (HbA1c) are associated with higher cardiovascular risk. The relation between undetected diabetes and clinical outcome after percutaneous coronary intervention is unknown. To investigate whether these patients

  6. Fish hemoglobins

    Directory of Open Access Journals (Sweden)

    P.C. de Souza

    2007-06-01

    Full Text Available Vertebrate hemoglobin, contained in erythrocytes, is a globular protein with a quaternary structure composed of 4 globin chains (2 alpha and 2 beta and a prosthetic group named heme bound to each one. Having myoglobin as an ancestor, hemoglobin acquired the capacity to respond to chemical stimuli that modulate its function according to tissue requirements for oxygen. Fish are generally submitted to spatial and temporal O2 variations and have developed anatomical, physiological and biochemical strategies to adapt to the changing environmental gas availability. Structurally, most fish hemoglobins are tetrameric; however, those from some species such as lamprey and hagfish dissociate, being monomeric when oxygenated and oligomeric when deoxygenated. Fish blood frequently possesses several hemoglobins; the primary origin of this finding lies in the polymorphism that occurs in the globin loci, an aspect that may occasionally confer advantages to its carriers or even be a harmless evolutionary remnant. On the other hand, the functional properties exhibit different behaviors, ranging from a total absence of responses to allosteric regulation to drastic ones, such as the Root effect.

  7. A Comparison of Hemoglobin A2 Levels in Untreated and Treated Groups of HIV Patients on ART Including Zidovudine

    Directory of Open Access Journals (Sweden)

    Jitendra Singh Nigam

    2013-01-01

    Full Text Available Objective. To assess the level of haemoglobin A2 in HIV patients on antiretroviral therapy (ART including zidovudine with untreated HIV patients. Material and Methods. The study was a case control study. A total of 185 patients were included in the study; the case group included 125 HIV patients who were on antiretroviral therapy (ART including zidovudine and 60 were in the control group who were not receiving ART. The high-performance liquid chromatography (HPLC was done and hemoglobin A2 level was observed; value more than 3.5% was considered significant. The Hb A2 percentages of HIV patients were compared with those of control using an unpaired t-test. Results. The mean of Hb A2 in case group was 3.462% (SD 0.675 and in control group it was 2.815% (SD0.246. The higher Hb A2 value was seen in significant number of treated patients than control group (P<0.0001. Conclusion. The clinicians, pathologists, haematologists, and genetic counsellors should be aware of effects of nutritional anaemia and ART on Hb A2 to reduce the chances of misdiagnosis of β-thalassaemia especially in developing countries and for centres for antenatal screening.

  8. Therapeutic hemoglobin levels after gene transfer in β-thalassemia mice and in hematopoietic cells of β-thalassemia and sickle cells disease patients.

    Directory of Open Access Journals (Sweden)

    Laura Breda

    Full Text Available Preclinical and clinical studies demonstrate the feasibility of treating β-thalassemia and Sickle Cell Disease (SCD by lentiviral-mediated transfer of the human β-globin gene. However, previous studies have not addressed whether the ability of lentiviral vectors to increase hemoglobin synthesis might vary in different patients.We generated lentiviral vectors carrying the human β-globin gene with and without an ankyrin insulator and compared their ability to induce hemoglobin synthesis in vitro and in thalassemic mice. We found that insertion of an ankyrin insulator leads to higher, potentially therapeutic levels of human β-globin through a novel mechanism that links the rate of transcription of the transgenic β-globin mRNA during erythroid differentiation with polysomal binding and efficient translation, as reported here for the first time. We also established a preclinical assay to test the ability of this novel vector to synthesize adult hemoglobin in erythroid precursors and in CD34(+ cells isolated from patients affected by β-thalassemia and SCD. Among the thalassemic patients, we identified a subset of specimens in which hemoglobin production can be achieved using fewer copies of the vector integrated than in others. In SCD specimens the treatment with AnkT9W ameliorates erythropoiesis by increasing adult hemoglobin (Hb A and concurrently reducing the sickling tetramer (Hb S.Our results suggest two major findings. First, we discovered that for the purpose of expressing the β-globin gene the ankyrin element is particularly suitable. Second, our analysis of a large group of specimens from β-thalassemic and SCD patients indicates that clinical trials could benefit from a simple test to predict the relationship between the number of vector copies integrated and the total amount of hemoglobin produced in the erythroid cells of prospective patients. This approach would provide vital information to select the best candidates for these

  9. Hemoglobin C disease

    Science.gov (United States)

    Clinical hemoglobin C ... Hemoglobin C is an abnormal type of hemoglobin, the protein in red blood cells that carries oxygen. It is ... Americans. You are more likely to have hemoglobin C disease if someone in your family has had ...

  10. Is hemoglobin A1c level effective in predicting the prognosis of Fournier gangrene?

    Science.gov (United States)

    Sen, Haluk; Bayrak, Omer; Erturhan, Sakip; Borazan, Ersin; Koc, Mustafa Nihat

    2016-01-01

    Objectives: To evaluate the effect of immune failure and/or diabetes mellitus (DM) association on the mortality and morbidity of the Fournier's Gangrene (FG), and interrelatedly, the usability of HbA1c level in the prediction of prognosis. Materials and Methods: The data of 38 patients with the diagnosis of FG were investigated retrospectively. The patients were divided into two groups as patients with DM (Group 1, n = 18) and non-diabetics (Group 2, n = 20). The patients in group 1 were also divided into two subgroups as patients with HbA1c value ≥7 (Group 1a) and HbA1c value 38°C) (n = 22, 57.8%), purulent discharge from genital or perineal areas (n = 13, 34.2%), skin bruises (n = 11, 28.9%) and general state disorder in five patients that were admitted from day care center (13.1%). DM, as the most often comorbid disease, was detected in 18 patients (47.3%). Six patients (15.7%) were deceased during the follow-up period. Conclusion: In the present study, the researchers determined that diabetic patients with HbA1c level of 7 or higher had worse prognosis, and increased mortality. PMID:27453658

  11. GENETIC FACTORS INFLUENCING HEMOGLOBIN F LEVEL IN β-THALASSEMIA/HB E DISEASE.

    Science.gov (United States)

    Ruangrai, Waraporn; Jindadamrongwech, Sumalee

    2016-01-01

    Genetic factors influencing Hb F content in adult red blood cells include β-thalassemia genotypes, co-inheritance of α-thalassemia traits and single nucleotide polymorphisms (SNPs). Genotyping of α- and β-thalassemia and five SNPs in β-globin gene cluster previously identified in genome-wide association studies as being markers of elevated Hb F in β-thalassemia were performed in 81 subjects diagnosed with β-thalassemia/Hb E. Hb F levels are higher (0.9-7.1 g/dl) in subjects (n = 57) with the severe compared to mild β-thalassemia (0.8-2.5 g/ dl) (n = 4) genotypes, and are similarly low (0.7-3.5 g/dl) in those (n = 15) with α-thalassemia co-inheritance. Hb F levels in non-thalassemia controls (n = 150) range from 0 to 0.15 g/dl. The presence of homozygous minor alleles of the 5 SNPs are significant indicators of β-thalassemia/Hb E individuals with high Hb F (> 4 g/dl), independent of their thalassemia genotypes. Given that re-activation of γ-globin genes leads to amelioration of β-thalassemia severity, understanding how genetic factors up-regulate Hb F production may lead to possible therapeutic interventions, genetically or pharmacologically, of this debilitating disease in the not too distant future. PMID:27086429

  12. Effect of periodontal treatment on glycosylated hemoglobin levels in elderly patients with periodontal disease and type 2 diabetes

    Institute of Scientific and Technical Information of China (English)

    OU Long; LI Ru-fan

    2011-01-01

    Background Periodontal disease is closely related to type 2 diabetes and is an important complication of diabetes.This study aimed to investigate the effect of periodontal treatment on levels of blood glucose (Glu) and glycosylated hemoglobin (HbA1c) among elderly patients with type 2 diabetes and periodontal disease.Methods A total of 107 elderly patients with type 2 diabetes and periodontal disease were selected and divided into two groups according to their HbA1c levels. Group A was a well-controlled diabetic group and group B was uncontrolled.Their probing depth (PD), attachment loss (AL), the value of Glu and HbA1c were analyzed before periodontal treatment and 4 months later.Results There was a significant difference in periodontal condition between groups A and B (P <0.01). The periodontal condition for both groups was significantly (P<0.01) improved after periodontal therapy. The effect of treatment in group A was more pronounced than group B, and the difference was significant (P <0.01). After the periodontal treatment, Glu and HbA1c were reduced significantly in both groups (P <0.05).Conclusions Periodontal condition is related to the control of Glu level among elderly patients with type 2 diabetes and periodontal disease. Periodontal treatment can effectively reduce the level of Glu and HbA1c as well as improve the periodontal condition in elderly type-2 diabetes patients with periodontal disease.

  13. Locally Advanced Stage IV Squamous Cell Carcinoma of the Head and Neck: Impact of Pre-Radiotherapy Hemoglobin Level and Interruptions During Radiotherapy

    International Nuclear Information System (INIS)

    Purpose: Stage IV head and neck cancer patients carry a poor prognosis. Clear understanding of prognostic factors can help to optimize care for the individual patient. This study investigated 11 potential prognostic factors including pre-radiotherapy hemoglobin level and interruptions during radiotherapy for overall survival (OS), metastases-free survival (MFS), and locoregional control (LC) after radiochemotherapy. Methods and Materials: Eleven factors were investigated in 153 patients receiving radiochemotherapy for Stage IV squamous cell head and neck cancer: age, gender, Karnofsky performance score (KPS), tumor site, grading, T stage, N stage, pre-radiotherapy hemoglobin level, surgery, chemotherapy type, and interruptions during radiotherapy >1 week. Results: On multivariate analysis, improved OS was associated with KPS 90-100 (relative risk [RR], 2.36; 95% confidence interval [CI], 1.20-4.93; p = .012), hemoglobin ≥12 g/dL (RR, 1.88; 95% CI, 1.01-3.53; p = .048), and no radiotherapy interruptions (RR, 2.59; 95% CI, 1.15-5.78; p = .021). Improved LC was significantly associated with lower T stage (RR, 2.17; 95% CI, 1.16-4.63; p = .013), hemoglobin ≥12 g/dL (RR, 4.12; 95% CI, 1.92-9.09; p 1 week. It appears important to avoid anemia and radiotherapy interruptions to achieve the best treatment results

  14. Baseline blood levels of manganese, lead, cadmium, copper, and zinc in residents of Beijing suburb

    Energy Technology Data Exchange (ETDEWEB)

    Zhang, Long-Lian, E-mail: Longlian57@163.com [Department of Occupational Diseases Control and Prevention, Fengtai Center for Disease Control and Prevention, Beijing 100071 (China); Lu, Ling [Department of Occupational Diseases Control and Prevention, Fengtai Center for Disease Control and Prevention, Beijing 100071 (China); Pan, Ya-Juan; Ding, Chun-Guang [Institute for Occupational Health and Poison Control in China Center for Disease Prevention and Control, Beijing 100050 (China); Xu, Da-Yong [Department of Occupational Diseases Control and Prevention, Fengtai Center for Disease Control and Prevention, Beijing 100071 (China); Huang, Chuan-Feng; Pan, Xing-Fu [Institute for Occupational Health and Poison Control in China Center for Disease Prevention and Control, Beijing 100050 (China); Zheng, Wei, E-mail: wzheng@purdue.edu [School of Health Sciences, Purdue University, West Lafayette, IN 47907 (United States)

    2015-07-15

    Baseline blood concentrations of metals are important references for monitoring metal exposure in environmental and occupational settings. The purpose of this study was to determine the blood levels of manganese (Mn), copper (Cu), zinc (Zn), lead (Pb), and cadmium (Cd) among the residents (aged 12–60 years old) living in the suburb southwest of Beijing in China and to compare the outcomes with reported values in various developed countries. Blood samples were collected from 648 subjects from March 2009 to February 2010. Metal concentrations in the whole blood were determined by ICP-MS. The geometric means of blood levels of Mn, Cu, Zn, Pb and Cd were 11.4, 802.4, 4665, 42.6, and 0.68 µg/L, respectively. Male subjects had higher blood Pb than the females, while the females had higher blood Mn and Cu than the males. There was no gender difference for blood Cd and Zn. Smokers had higher blood Cu, Zn, and Cd than nonsmokers. There were significant age-related differences in blood levels of all metals studied; subjects in the 17–30 age group had higher blood levels of Mn, Pb, Cu, and Zn, while those in the 46–60 age group had higher Cd than the other age groups. A remarkably lower blood level of Cu and Zn in this population as compared with residents of other developed countries was noticed. Based on the current study, the normal reference ranges for the blood Mn were estimated to be 5.80–25.2 μg/L; for blood Cu, 541–1475 μg/L; for blood Zn, 2349–9492 μg/L; for blood Pb, <100 μg/L; and for blood Cd, <5.30 μg/L in the general population living in Beijing suburbs. - Highlights: • Baseline blood levels of metals in residents of Beijing suburb are investigated. • BMn and BPb in this cohort are higher than those in other developed countries. • Remarkably lower blood levels of Cu and Zn in this Chinese cohort are noticed. • The reference values for blood levels of Mn, Cu, Zn, Pb, and Cd are established.

  15. Mean hemoglobin levels in venous blood samples and prevalence of anemia in Japanese elementary and junior high school students

    International Nuclear Information System (INIS)

    Screening for anemia has been performed in schools in Japan for over 30 years. The long-term effect of the nuclear power plant disaster on the prevalence of anemia in school age children is unknown. This research was performed to evaluate the prevalence of anemia in school age children and to determine grade-level and gender-related reference hemoglobin (Hb) levels prior to the nuclear disaster. Data for this research were obtained from results of screening for anemia obtained by venous blood sampling in schools in 2002. Mean Hb levels were calculated for each grade level (elementary school grades 1-6 and junior high school years 1-3) and according to gender, and the prevalence of anemia was determined. In our research, Tokyo Health Service Association guidelines were used to determine reference Hb levels for anemia. We demonstrated that Hb levels in boys increased with age during childhood and adolescence (from 13.1±0.7 g/dL in 7 year olds to 14.9±1.1 g/dL in 15 year olds); in girls, Hb levels peaked at menarche (13.7±0.8 g/dL in 12 year olds), decreasing slightly thereafter (13.4±1.1 g/dL in 15 year olds). The prevalence of anemia was 0.26% in elementary school boys, 0.27% in elementary school girls, and 1.21% in junior high school boys. The prevalence of anemia in second- and third-year junior high school girls was lower than that in first-year junior high school girls. Among all junior high school girls, 5.73% had mild anemia. Iron-deficiency anemia is the commonest type of anemia in high school girls, secondary to the relative lack of iron due to menstruation, the growth spurt and exercise. Appropriate dietary therapy and treatment of anemia, together with education about the dietary prevention of anemia, are important to reduce the prevalence of anemia in high school students. When complete blood counts are performed in regions thought to be affected by the Fukushima nuclear power plant disaster, our report can serve as a reference during evaluation of Hb

  16. The Relationship between Serum Hemoglobin and Creatinine Levels and Intra-Hospital Mortality and Morbidity in Acute Myocardial Infarction

    Directory of Open Access Journals (Sweden)

    Afsoon Fazlinezhad

    2014-09-01

    Full Text Available Background: Studies have shown that Glomerular Filtration Rate (GFR and Hemoglobin (Hb concentrations are two predictive values for ST-elevation Myocardial Infarction (MI mortality.. Objectives: This study aimed to investigate the relationship between GFR and Hb concentrations and intra-hospital mortality and electrocardiographic (ECG and echocardiographic abnormalities in ST-elevation MI patients admitted to a highly equipped hospital in Mashhad. The results will help define some factors to manage these patients more efficiently.. Patients and Methods: This descriptive study aimed to assess the relationship between Hb and GFR concentrations and mortality and morbidity among 294 randomly selected patients with ST-elevation MI. Echocardiography, ECG, and routine laboratory tests, including Hb and creatinine, were performed for all the patients. Then, the data were entered into the SPSS statistical software, version 16 and were analyzed using chi-square, t-test, and ANOVA. P < 0.05 was considered as statistically significant.. Results: Intra-hospital mortality rate was 10.5%. Besides, the results showed higher levels of serum blood sugar (P < 0.001, higher levels of creatinine (P < 0.001, lower levels of GFR (P < 0.001, lower ejection fraction (P < 0.001, higher grades of left ventricular diastolic dysfunction (P = 0.002, and lower mean Hb concentration (P = 0.022 in the dead compared to the alive cases. Besides, the patients with mechanical complications had lower Hb levels (P = 0.008. The results showed no significant relationship between creatinine level and mechanical and electrical complications (P = 0.430 and P = 0.095, respectively. However, ejection fraction was significantly associated with GFR (P = 0.016.. Conclusions: According to the results, low levels of Hb and GFR could predict mortality caused by ST-elevation MI and ECG abnormalities could notify intra-hospital death. Moreover, lower Hb levels were associated with mechanical

  17. Decreased serum glucose and glycosylated hemoglobin levels in patients with Chuvash polycythemia: a role for HIF in glucose metabolism.

    Science.gov (United States)

    McClain, Donald A; Abuelgasim, Khadega A; Nouraie, Mehdi; Salomon-Andonie, Juan; Niu, Xiaomei; Miasnikova, Galina; Polyakova, Lydia A; Sergueeva, Adelina; Okhotin, Daniel J; Cherqaoui, Rabia; Okhotin, David; Cox, James E; Swierczek, Sabina; Song, Jihyun; Simon, M Celeste; Huang, Jingyu; Simcox, Judith A; Yoon, Donghoon; Prchal, Josef T; Gordeuk, Victor R

    2013-01-01

    In Chuvash polycythemia, a homozygous 598C>T mutation in the von Hippel-Lindau gene (VHL) leads to an R200W substitution in VHL protein, impaired degradation of α-subunits of hypoxia-inducible factor (HIF)-1 and HIF-2, and augmented hypoxic responses during normoxia. Chronic hypoxia of high altitude is associated with decreased serum glucose and insulin concentrations. Other investigators reported that HIF-1 promotes cellular glucose uptake by increased expression of GLUT1 and increased glycolysis by increased expression of enzymes such as PDK. On the other hand, inactivation of Vhl in murine liver leads to hypoglycemia associated with a HIF-2-related decrease in the expression of the gluconeogenic enzyme genes Pepck, G6pc, and Glut2. We therefore hypothesized that glucose concentrations are decreased in individuals with Chuvash polycythemia. We found that 88 Chuvash VHL ( R200W ) homozygotes had lower random glucose and glycosylated hemoglobin A1c levels than 52 Chuvash subjects with wild-type VHL alleles. Serum metabolomics revealed higher glycerol and citrate levels in the VHL ( R200W ) homozygotes. We expanded these observations in VHL ( R200W ) homozygote mice and found that they had lower fasting glucose values and lower glucose excursions than wild-type control mice but no change in fasting insulin concentrations. Hepatic expression of Glut2 and G6pc, but not Pdk2, was decreased, and skeletal muscle expression of Glut1, Pdk1, and Pdk4 was increased. These results suggest that both decreased hepatic gluconeogenesis and increased skeletal uptake and glycolysis contribute to the decreased glucose concentrations. Further study is needed to determine whether pharmacologically manipulating HIF expression might be beneficial for treatment of diabetic patients. PMID:23015148

  18. Convergent Evolution of Hemoglobin Function in High-Altitude Andean Waterfowl Involves Limited Parallelism at the Molecular Sequence Level.

    Directory of Open Access Journals (Sweden)

    Chandrasekhar Natarajan

    2015-12-01

    Full Text Available A fundamental question in evolutionary genetics concerns the extent to which adaptive phenotypic convergence is attributable to convergent or parallel changes at the molecular sequence level. Here we report a comparative analysis of hemoglobin (Hb function in eight phylogenetically replicated pairs of high- and low-altitude waterfowl taxa to test for convergence in the oxygenation properties of Hb, and to assess the extent to which convergence in biochemical phenotype is attributable to repeated amino acid replacements. Functional experiments on native Hb variants and protein engineering experiments based on site-directed mutagenesis revealed the phenotypic effects of specific amino acid replacements that were responsible for convergent increases in Hb-O2 affinity in multiple high-altitude taxa. In six of the eight taxon pairs, high-altitude taxa evolved derived increases in Hb-O2 affinity that were caused by a combination of unique replacements, parallel replacements (involving identical-by-state variants with independent mutational origins in different lineages, and collateral replacements (involving shared, identical-by-descent variants derived via introgressive hybridization. In genome scans of nucleotide differentiation involving high- and low-altitude populations of three separate species, function-altering amino acid polymorphisms in the globin genes emerged as highly significant outliers, providing independent evidence for adaptive divergence in Hb function. The experimental results demonstrate that convergent changes in protein function can occur through multiple historical paths, and can involve multiple possible mutations. Most cases of convergence in Hb function did not involve parallel substitutions and most parallel substitutions did not affect Hb-O2 affinity, indicating that the repeatability of phenotypic evolution does not require parallelism at the molecular level.

  19. Convergent Evolution of Hemoglobin Function in High-Altitude Andean Waterfowl Involves Limited Parallelism at the Molecular Sequence Level.

    Science.gov (United States)

    Natarajan, Chandrasekhar; Projecto-Garcia, Joana; Moriyama, Hideaki; Weber, Roy E; Muñoz-Fuentes, Violeta; Green, Andy J; Kopuchian, Cecilia; Tubaro, Pablo L; Alza, Luis; Bulgarella, Mariana; Smith, Matthew M; Wilson, Robert E; Fago, Angela; McCracken, Kevin G; Storz, Jay F

    2015-12-01

    A fundamental question in evolutionary genetics concerns the extent to which adaptive phenotypic convergence is attributable to convergent or parallel changes at the molecular sequence level. Here we report a comparative analysis of hemoglobin (Hb) function in eight phylogenetically replicated pairs of high- and low-altitude waterfowl taxa to test for convergence in the oxygenation properties of Hb, and to assess the extent to which convergence in biochemical phenotype is attributable to repeated amino acid replacements. Functional experiments on native Hb variants and protein engineering experiments based on site-directed mutagenesis revealed the phenotypic effects of specific amino acid replacements that were responsible for convergent increases in Hb-O2 affinity in multiple high-altitude taxa. In six of the eight taxon pairs, high-altitude taxa evolved derived increases in Hb-O2 affinity that were caused by a combination of unique replacements, parallel replacements (involving identical-by-state variants with independent mutational origins in different lineages), and collateral replacements (involving shared, identical-by-descent variants derived via introgressive hybridization). In genome scans of nucleotide differentiation involving high- and low-altitude populations of three separate species, function-altering amino acid polymorphisms in the globin genes emerged as highly significant outliers, providing independent evidence for adaptive divergence in Hb function. The experimental results demonstrate that convergent changes in protein function can occur through multiple historical paths, and can involve multiple possible mutations. Most cases of convergence in Hb function did not involve parallel substitutions and most parallel substitutions did not affect Hb-O2 affinity, indicating that the repeatability of phenotypic evolution does not require parallelism at the molecular level.

  20. Low Levels of Hemoglobin at Admission Are Associated With Increased 30-Day Mortality in Patients With Hip Fracture

    DEFF Research Database (Denmark)

    Praetorius, Katrine; Madsen, Christian M; Abrahamsen, Bo;

    2016-01-01

    INTRODUCTION: Previous smaller studies suggest that anemia is a risk factor for mortality in patients with hip fracture. The purpose of this investigation was to assess the correlation between hemoglobin at admission with 30-day mortality following a hip fracture in a large-scale study. PATIENTS...... AND METHODS: From January 1996 to December 2012, all patients with hip fracture (>60 years of age) admitted to Bispebjerg Hospital, Copenhagen, were identified from a local hip fracture database. We excluded conservatively treated patients and patients who died preoperatively. RESULTS: Seven thousand four...... hundred twenty-one consecutive patients with hip fracture were identified. Of those 7319 had a hemoglobin measurement on admission and were thus eligible for further analysis. Mean hemoglobin for patients alive at 30 days was 7.6 (standard deviation [SD]: 1.0) and for deceased patients 7.4 (SD: 1.1), P...

  1. How Should We Be Determining Background and Baseline Antibiotic Resistance Levels in Agroecosystem Research?

    Science.gov (United States)

    Rothrock, Michael J; Keen, Patricia L; Cook, Kimberly L; Durso, Lisa M; Franklin, Alison M; Dungan, Robert S

    2016-03-01

    Although historically, antibiotic resistance has occurred naturally in environmental bacteria, many questions remain regarding the specifics of how humans and animals contribute to the development and spread of antibiotic resistance in agroecosystems. Additional research is necessary to completely understand the potential risks to human, animal, and ecological health in systems altered by antibiotic-resistance-related contamination. At present, analyzing and interpreting the effects of human and animal inputs on antibiotic resistance in agroecosystems is difficult, since standard research terminology and protocols do not exist for studying background and baseline levels of resistance in the environment. To improve the state of science in antibiotic-resistance-related research in agroecosystems, researchers are encouraged to incorporate baseline data within the study system and background data from outside the study system to normalize the study data and determine the potential impact of antibiotic-resistance-related determinants on a specific agroecosystem. Therefore, the aims of this review were to (i) present standard definitions for commonly used terms in environmental antibiotic resistance research and (ii) illustrate the need for research standards (normalization) within and between studies of antibiotic resistance in agroecosystems. To foster synergy among antibiotic resistance researchers, a new surveillance and decision-making tool is proposed to assist researchers in determining the most relevant and important antibiotic-resistance-related targets to focus on in their given agroecosystems. Incorporation of these components within antibiotic-resistance-related studies should allow for a more comprehensive and accurate picture of the current and future states of antibiotic resistance in the environment. PMID:27065388

  2. Role of Breastfeeding and Complementary Food on Hemoglobin and Ferritin Levels in a Cambodian Cross-Sectional Sample of Children Aged 3 to 24 Months.

    Directory of Open Access Journals (Sweden)

    Anika Reinbott

    Full Text Available Iron deficiency derives from a low intake of dietary iron, poor absorption of iron, and high requirements due to growth as well as blood loss. An estimated number of about 50% of all anemia may be attributed to iron deficiency among young children in Cambodia.A cross-sectional survey was conducted in rural Cambodia in September 2012. Villages in pre-selected communes were randomly chosen using stunting as a primary indicator of nutritional status. In total, 928 randomly selected households with children aged 3-23 months were included. Hemoglobin, ferritin, soluble transferrin receptor (sTfR, and retinol binding protein (RBP were assessed from capillary blood samples. In addition, length/height and weight of mothers and children were taken and data on dietary diversity was collected. A child feeding index (CFI was created. Associations between biomarkers of iron and vitamin A status and nutritional status or food intake were explored.Anemia prevalence was highest among 6- to 12-months-olds (71%. Ferritin and sTfR inversely correlated and were significantly associated with hemoglobin concentrations. The consumption of animal source foods (ASF significantly impacts on the interaction between ferritin, sTfR and hemoglobin. Concentrations of RBP were significantly higher in children who had received a vitamin A supplement. The CFI was associated with sTfR and hemoglobin. Lower length and weight were associated with lower ferritin levels and showed an indirect effect on hemoglobin through ferritin.Nutrition programs targeting children under 2 years of age need to focus on the preparation of complementary foods with high nutrient density to sustainably prevent micronutrient deficiency and generally improve nutritional status. Future assessments of the micronutrient status should include identification of hemoglobinopathies and parasitic infections to better understand all causes of anemia in Cambodian infants and young children.German Clinical Trials

  3. Comparison of glycosylated hemoglobin (HbA1C levels in patients with chronic periodontitis and healthy controls

    Directory of Open Access Journals (Sweden)

    Padma Rajan

    2013-01-01

    Conclusion: Chronic periodontitis is associated with a slight elevation in glycosylated hemoglobin (lab and chair side kit and that the clinical significance of this difference remains to be determined. This preliminary finding is consistent with earlier reports that chronic periodontitis is associated with elevated blood glucose in adults without diabetes and may increase one′s risk for type-2 diabetes.

  4. Prognostic impact of hemoglobin level and other factors in patients with high-grade gliomas treated with postoperative radiochemotherapy and sequential chemotherapy based on temozolomide. A 10-year experience at a single institution

    Energy Technology Data Exchange (ETDEWEB)

    Ausili Cefaro, Giampiero; Genovesi, Domenico; Vinciguerra, Annamaria; Trignani, Marianna; Taraborrelli, Maria; Augurio, Antonietta [G. d' Annunzio Univ. of Chieti, SS. Annunziata Hospital, Chieti (Italy). Dept. of Radiation Oncology; Buonaguidi, Roberto [G. d' Annunzio Univ. of Chieti, Spirito Santo Hospital, Pescara (Italy). Dept. of Neurosurgery; Galzio, Renato J. [Univ. of L' Aquila, San Salvatore Hospital, L' Aquila (Italy). Dept. of Operative Unit of Neurosurgery and Health Sciences; Di Nicola, Marta [G. d' Annunzio Univ. of Chieti (Italy). Lab. of Biostatistics

    2011-12-15

    To evaluate the influence of serum hemoglobin level prior to radiotherapy and other prognostic factors on survival in patients with high-grade gliomas. From 2001-2010, we retrospectively evaluated a total of 48 patients with malignant glioma treated with surgery and postoperative radiochemotherapy with temozolomide. A total of 37 of 48 patients received sequential temozolomide. Hemoglobin levels were assayed before radiotherapy in all patients. The Kaplan-Meier method was applied to estimate the overall survival, while the log-rank test was applied to evaluate the differences on survival probability between prognostic subgroups. Results were assessed in 43 patients. The median overall survival time was 18 months (95% confidence interval: 12-40 months). The 1- and 2-year survival rates were 62.2% and 36.3%, respectively. The prognostic factors analyzed were gender, age, extent of surgery, performance status before and after radiotherapy, sequential chemotherapy, hemoglobin level, and methylation of the O-6-methylguanine-DNA methyltransferase gene (MGMT). In univariate analysis, the variables significantly related to survival were performance status before and after radiotherapy, sequential chemotherapy, and hemoglobin level. The median overall survival in patients with a hemoglobin level {<=} 12 g/dl was 12 months and 23 months in patients with a hemoglobin level > 12 g/dl. The 1- and 2-year survival rates were 46.7% and 20.0%, respectively, for patients with a hemoglobin level {<=} 12 mg/dl and 69.6% and 45.7%, respectively, for patients with a hemoglobin level > 12 g/dl. Our results confirm the impact of well-known prognostic factors on survival. In this research, it was found that a low hemoglobin level before radiotherapy can adversely influence the prognosis of patients with malignant gliomas.

  5. Baseline levels and temporal stability of 27 multiplexed serum cytokine concentrations in healthy subjects.

    Directory of Open Access Journals (Sweden)

    Angelique Biancotto

    Full Text Available BACKGROUND: Cytokines are humoral molecules that elicit regulatory function in immunologic pathways. The level and type of cytokine production has become critical in distinguishing physiologic from pathologic immune conditions. Cytokine profiling has become an important biomarker discovery tool in monitoring of the immune system. However, the variations in cytokine levels in individual subjects over time in healthy individuals have not been extensively studied. In this study, we use multiplex bead arrays to evaluate 27 analytes in paired serum samples taken seven days apart from 144 healthy individuals in order to assess variations over a short time period. METHODS: Fluorescent bead-based immunoassay (Luminex was used to measure 27 analytes in serum samples. Measurements were performed on matched samples from 144 healthy donors. To assess inter-plate variability, one arbitrarily selected serum sample was analyzed on each of the first ten plates as bridge sample. RESULTS: Using the bridge sample, we showed minimal inter-plate variations in the measurement of most analytes. In measurement of cytokines from the 144 patients at two time points, we found that three cytokines (IL-2, IL-15 and GM-CSF were undetectable and five analytes (RANTES, MCP-1, VEGF, MIP-1β and PDGF-BB showed significant difference in concentrations at Day 0 compared to Day 7. CONCLUSIONS: The current study demonstrated higher variations in cytokine levels among individuals than were observed for samples obtained one week apart from identical donors. These data suggest that a serum sample from each subject for use as a baseline measurement is a better control for clinical trials rather than sera from a paired cohort.

  6. Hair as a meaningful measure of baseline cortisol levels over time in dogs.

    Science.gov (United States)

    Bryan, Heather M; Adams, Amanda G; Invik, Rosemary M; Wynne-Edwards, Katherine E; Smits, Judit E G

    2013-03-01

    Cortisol measurements of hair are becoming a valuable tool in monitoring chronic stress. To further validate this approach in domestic dogs, we compared the variability of cortisol immunoreactivity in hair with that in saliva and feces of dogs housed under constant social and physical conditions. Fecal (n = 268), and hair (n = 21) samples were collected over 3 mo from 7 dogs housed in a kennel and kept for training veterinary students in minimally invasive procedures. Salivary samples (n = 181) were collected 3 times daily twice weekly during the last month of the study. Hair and salivary samples were analyzed by enzyme immunoassay and feces by radioimmunoassay. HPLC coupled with tandem mass spectrometry was used to confirm the presence of cortisol in 3 hair samples. Variability of cortisol was compared across sample types by using repeated-measures ANOVA followed by paired t tests. Within dogs, cortisol immunoreactivity was less variable in hair than in saliva or feces. Averaged over time, the variability of fecal samples approached that of hair when feces were collected at least 4 times monthly. As predicted, the stable social and environmental condition of the dogs maintained repeatability over time and supported the hypothesis that data from hair samples reflect baseline cortisol levels. These findings indicate that determining cortisol immunoreactivity in hair is a more practical approach than is using samples of saliva or feces in monitoring the effects of long-term stressors such as social or physical environments and disease progression. PMID:23562104

  7. Level 3 baseline risk evaluation for Building 3506 at Oak Ridge National Laboratory, Oak Ridge, Tennessee

    Energy Technology Data Exchange (ETDEWEB)

    Golden, K.M.; Robers, S.K.; Cretella, F.M.

    1994-12-01

    This report presents the results of the Level 3 Baseline Risk Evaluation (BRE) performed on Building 3506 located at the Oak Ridge National Laboratory (ORNL). This BRE is intended to provide an analysis of the potential for adverse health effects (current or future) posed by contaminants at the facility. The decision was made to conduct a Level 3 (least rigorous) BRE because only residual contamination exists in the building. Future plans for the facility (demolition) also preclude a rigorous analysis. Site characterization activities for Building 3506 were conducted in fall of 1993. Concrete core samples were taken from the floors and walls of both the cell and the east gallery. These cores were analyzed for radionuclides and organic and inorganic chemicals. Smear samples and direct radiation measurements were also collected. Sediment exists on the floor of the cell and was also analyzed. To adequately characterize the risks posed by the facility, receptors for both current and potential future land uses were evaluated. For the current land use conditions, two receptors were evaluated. The first receptor is a hypothetical maintenance worker who spends 250 days (8 hours/day) for 25 years working in the facility. The remaining receptor evaluated is a hypothetical S and M worker who spends 2 days (8 hours/day) per year for 25 years working within the facility. This particular receptor best exemplifies the current worker scenario for the facility. The two current exposure scenarios and parameters of exposure (e.g., inhalation and ingestion rates) have been developed to provide a conservative (i.e. health protective) estimate of potential exposure.

  8. Level 3 baseline risk evaluation for Building 3506 at Oak Ridge National Laboratory, Oak Ridge, Tennessee

    International Nuclear Information System (INIS)

    This report presents the results of the Level 3 Baseline Risk Evaluation (BRE) performed on Building 3506 located at the Oak Ridge National Laboratory (ORNL). This BRE is intended to provide an analysis of the potential for adverse health effects (current or future) posed by contaminants at the facility. The decision was made to conduct a Level 3 (least rigorous) BRE because only residual contamination exists in the building. Future plans for the facility (demolition) also preclude a rigorous analysis. Site characterization activities for Building 3506 were conducted in fall of 1993. Concrete core samples were taken from the floors and walls of both the cell and the east gallery. These cores were analyzed for radionuclides and organic and inorganic chemicals. Smear samples and direct radiation measurements were also collected. Sediment exists on the floor of the cell and was also analyzed. To adequately characterize the risks posed by the facility, receptors for both current and potential future land uses were evaluated. For the current land use conditions, two receptors were evaluated. The first receptor is a hypothetical maintenance worker who spends 250 days (8 hours/day) for 25 years working in the facility. The remaining receptor evaluated is a hypothetical S and M worker who spends 2 days (8 hours/day) per year for 25 years working within the facility. This particular receptor best exemplifies the current worker scenario for the facility. The two current exposure scenarios and parameters of exposure (e.g., inhalation and ingestion rates) have been developed to provide a conservative (i.e. health protective) estimate of potential exposure

  9. Lack of influence of fetal hemoglobin levels or erythrocyte indices on the severity of sickle cell anemia.

    OpenAIRE

    Powars, D R; Schroeder, W. A.; Weiss, J N; Chan, L S; Azen, S P

    1980-01-01

    Persons with sickle cell anemia who have elevated fetal hemoglobin or lowered erythrocyte mean corpuscular volume are reputed to have less severe clinical manifestations and a greater probability of survival. This study examines the relationship between seven clinical indicators of morbidity in sickle cell anemia and seven hematological parameters that were collected from 214 patients. Risks of sickle cell crisis, acute chest syndrome, hospital admissions, cerebrovascular accident, aseptic ne...

  10. Impact of baseline lipoprotein and C-reactive protein levels on coronary atheroma regression following high-intensity statin therapy.

    Science.gov (United States)

    Puri, Rishi; Nissen, Steven E; Shao, Mingyuan; Uno, Kiyoko; Kataoka, Yu; Kapadia, Samir R; Tuzcu, E Murat; Nicholls, Stephen J

    2014-11-15

    Guidelines now recommend high-intensity statin therapy in all patients with proven atherosclerotic disease. Yet the impact of baseline lipoprotein and C-reactive protein (CRP) levels on measures of disease regression to this therapy are unknown. The aim of this study was to test the hypothesis that high-intensity statin therapy causes equivalent degrees of coronary atheroma regression irrespective of baseline lipoprotein and CRP levels. In 8 prospective randomized trials using serial coronary intravascular ultrasound, 1,881 patients who maintained or switched to 18- to 24 months of high-intensity statin therapy (rosuvastatin 40 mg or atorvastatin 80 mg) were stratified according to baseline lipoprotein and CRP levels. Changes in coronary percentage atheroma volume (PAV) and total atheroma volume (TAV) were evaluated. High-intensity statin therapy produced significant reductions from baseline in low-density lipoprotein cholesterol by 38.4%, non-high-density lipoprotein (HDL) cholesterol by 33.6%, triglycerides by 13.1%, and CRP by 33.3%, while increasing HDL cholesterol by 11.7% (p low-density lipoprotein cholesterol, HDL cholesterol, non-HDL cholesterol, triglycerides, or CRP. Moreover, across all measured lipoproteins and CRP, most patients demonstrated plaque regression (defined as any change from baseline in PAV or TAV coronary atherosclerosis in all strata of patients with coronary artery disease irrespective of baseline lipoprotein or CRP levels. These findings provide support for the latest United States guideline recommendations for the broad use of high-intensity statin therapy in all patients with atherosclerosis, regardless of baseline lipid status.

  11. Level III baseline risk evaluation for Building 3505 at Oak Ridge National Laboratory, Oak Ridge, Tennessee

    International Nuclear Information System (INIS)

    The Level III Baseline Risk Evaluation (BRE) for Building 3505, the ORNL Metal Recovery Facility, provides an analysis of the potential for adverse health effects, current or future, associated with the presence of hazardous substances in the building. The Metal Recovery Facility was used from 1952 through 1960 to process large quantities of radioactive material using the PUREX process for the recovery of uranium-238, plutonium-239, neptunium-237, and americium-241. The facility consists of seven process cells (A through G), a canal, a dissolver room, a dissolver pit, an office, locker room, storage area, control room, electrical gallery, shop, and makeup area. The cells were used to house the nuclear fuel reprocessing equipment, and the canal was constructed to be used as a water-shielded transfer canal. Currently, there are no known releases of radioactive contaminants from Building 3505. To perform the BRE, historical radiological survey data were used to estimate the concentration of alpha- and beta/gamma emitting radionuclides in the various cells, rooms, and other areas in Building 3505. Data from smear surveys were used to estimate the amount of transferable contamination (to which receptors can be exposed via inhalation and ingestion), and data from probe surveys were used to estimate the amount of both fixed and transferable contamination (from which receptors can receive external exposure). Two land use scenarios, current and future, and their subsequent exposure scenarios were explored in the BRE. Under the current land use scenario, two exposure scenarios were evaluated. The first was a worst-case industrial exposure scenario in which the receptor is a maintenance worker who works 8 hours/day, 350 days/year in the building for 25 years. In the second, more realistic exposure scenario, the receptor is a surveillance and maintenance (S ampersand M) worker who spends two 8-hour days/year in the building for 25 years

  12. Level III baseline risk evaluation for Building 3505 at Oak Ridge National Laboratory, Oak Ridge, Tennessee

    Energy Technology Data Exchange (ETDEWEB)

    Mostella, W.B. Jr.

    1994-12-01

    The Level III Baseline Risk Evaluation (BRE) for Building 3505, the ORNL Metal Recovery Facility, provides an analysis of the potential for adverse health effects, current or future, associated with the presence of hazardous substances in the building. The Metal Recovery Facility was used from 1952 through 1960 to process large quantities of radioactive material using the PUREX process for the recovery of uranium-238, plutonium-239, neptunium-237, and americium-241. The facility consists of seven process cells (A through G), a canal, a dissolver room, a dissolver pit, an office, locker room, storage area, control room, electrical gallery, shop, and makeup area. The cells were used to house the nuclear fuel reprocessing equipment, and the canal was constructed to be used as a water-shielded transfer canal. Currently, there are no known releases of radioactive contaminants from Building 3505. To perform the BRE, historical radiological survey data were used to estimate the concentration of alpha- and beta/gamma emitting radionuclides in the various cells, rooms, and other areas in Building 3505. Data from smear surveys were used to estimate the amount of transferable contamination (to which receptors can be exposed via inhalation and ingestion), and data from probe surveys were used to estimate the amount of both fixed and transferable contamination (from which receptors can receive external exposure). Two land use scenarios, current and future, and their subsequent exposure scenarios were explored in the BRE. Under the current land use scenario, two exposure scenarios were evaluated. The first was a worst-case industrial exposure scenario in which the receptor is a maintenance worker who works 8 hours/day, 350 days/year in the building for 25 years. In the second, more realistic exposure scenario, the receptor is a surveillance and maintenance (S&M) worker who spends two 8-hour days/year in the building for 25 years.

  13. Revised CDM baseline study on fuel use and manure management at household level

    Energy Technology Data Exchange (ETDEWEB)

    Buysman, E.; Bryan, S.; Pino, M.

    2010-05-15

    This report presents the revised study of the original CDM baseline study conducted in 2006. The original study was conducted under the authority of the National Biogas Program (NBP), to study the potential GHG mitigation resulting from the adoption of domestic biodigesters. In the beginning of June 2006, a survey amongst 300 randomly selected households with the technical potential for a biodigester was conducted in the NBP's 6-targeted provinces (Kampong Cham, Svay Rieng, Prey Veng, Kampong Speu, Takeo and Kandal) in southeast Cambodia. The revised baseline study includes two additional provinces, Kampot and Kampong Chhnang. The survey showed that a significant proportion of the households have no access to basic sanitation and often have health problems. They consume mainly wood as cooking fuel and the majority use inefficient cooking stoves. The main lighting fuel is kerosene. The GHG emissions were calculated for each type of Animal Waste Management System (AWMS) and the baseline fuel consumption. The main methodology used is the GS-VER biodigester methodology and the IPCC 2006 guidelines to ex-ante estimate baseline, project and the emission reductions. The GHG emission from wood burning is only considered when it originates from a non-renewable source. The NRB analysis determined a NRB share of 70.7% for both collected and purchased wood. Total GHG emission is calculated by combining AWMS and wood fuels emissions. The annual baseline and project emission was estimated to be respectively 5.38 tCO2eq and 0.46 tCO2eq per average household, the emission reductions (ER) are therefore 4.92 tCO2eq/household/year.

  14. Effect of Smoking During Radiotherapy, Respiratory Insufficiency, and Hemoglobin Levels on Outcome in Patients Irradiated for Non-Small-Cell Lung Cancer

    International Nuclear Information System (INIS)

    Purpose: To investigate the effect of smoking during radiotherapy (RT), respiratory insufficiency before RT, hemoglobin levels during RT, and additional factors on overall survival, locoregional control (LRC), and metastasis-free survival in non-small-cell lung cancer patients. Methods and Materials: The following factors were investigated in 181 patients who underwent RT for non-small-cell lung cancer: age, gender, Karnofsky performance score, histologic type, grade, T/N stage, American Joint Committee on Cancer stage, surgery, chemotherapy, respiratory insufficiency before RT, pack-years, smoking during RT, and hemoglobin levels during RT. Additionally, in the 129 patients who did not undergo surgery, the effect of the equivalent dose in 2-Gy fractions (EQD2) (60 Gy) on outcome was investigated. Results: On multivariate analysis, improved overall survival was associated with a lower T stage (p = 0.004), lower N stage (p 0.040), surgery (p = 0.010), and no respiratory insufficiency (p = 0.023). A Karnofsky performance score >70 achieved borderline significance (p = 0.056). Improved LRC was associated with a lower T stage (p = 0.007) and no smoking during RT (p = 0.029). Improved metastasis-free survival was associated with lower T stage (p 60 Gy resulted in better LRC than did an EQD2 of ≤60 Gy. Conclusions: Smoking during RT had a significant effect on LRC, but we did not find that hemoglobin levels or respiratory insufficiency significantly affected LRC or metastasis-free survival in our patient population. Furthermore, our data suggest a dose-effect relationship in those patients who did not undergo surgery

  15. Major decrements in glycated hemoglobin levels between 1978 and 1989 in patients with insulin-dependent diabetes mellitus.

    Science.gov (United States)

    Arfken, C L; Schmidt, L E; McGill, J B; White, N H; Santiago, J V

    1996-01-01

    The Diabetes Control and Complications Trial has shown that intensive treatment can deter the development and progression of diabetic complications. Integral to intensive treatment is improved glycemic control. To describe the trend in glycemic control for subjects with insulin-dependent diabetes mellitus, we examined the medical records of 662 subjects seen between 1978 and 1989 at the Model Demonstration Unit of the Diabetes Research and Training Center (Washington University School of Medicine). Mean value of glycated hemoglobin showed steady decline from a peak of 11.5% in 1979 to 9.0% in 1989. This decline was observed both in subjects evaluated only once (annual rate of decline estimated from linear regression, -0.17 +/1 0.03; p = 0.0001) and in subjects evaluated more than once (annual rate of decline estimated from growth curves, -0.18 +/- 0.06; p = 0.0001). These results suggest that substantial lowering of glycated hemoglobin has occurred during the last decade. This reduction should result in a lowered risk of diabetic complications.

  16. Knowledge, attitude and practices of pregnant women regarding anemia, iron rich diet and iron supplements and its impact on their hemoglobin levels

    Directory of Open Access Journals (Sweden)

    Nivedita K.

    2016-02-01

    Conclusions: The present study indicated the lack of knowledge regarding anemia, iron rich foods and the importance of iron supplementation during pregnancy. Targeted estimation of hemoglobin levels in adolescent girls and women in reproductive age group, intensive counseling and motivation of pregnant women to consume Iron and folic acid and ensuring adequate supply to them, intensive de-worming, provision of toilet facilities to all households would help in reducing the incidence of anemia in pregnant women. [Int J Reprod Contracept Obstet Gynecol 2016; 5(2.000: 425-431

  17. Cortisol and politics: variance in voting behavior is predicted by baseline cortisol levels.

    Science.gov (United States)

    French, Jeffrey A; Smith, Kevin B; Alford, John R; Guck, Adam; Birnie, Andrew K; Hibbing, John R

    2014-06-22

    Participation in electoral politics is affected by a host of social and demographics variables, but there is growing evidence that biological predispositions may also play a role in behavior related to political involvement. We examined the role of individual variation in hypothalamic-pituitary-adrenal (HPA) stress axis parameters in explaining differences in self-reported and actual participation in political activities. Self-reported political activity, religious participation, and verified voting activity in U.S. national elections were collected from 105 participants, who were subsequently exposed to a standardized (nonpolitical) psychosocial stressor. We demonstrated that lower baseline salivary cortisol in the late afternoon was significantly associated with increased actual voting frequency in six national elections, but not with self-reported non-voting political activity. Baseline cortisol predicted significant variation in voting behavior above and beyond variation accounted for by traditional demographic variables (particularly age of participant in our sample). Participation in religious activity was weakly (and negatively) associated with baseline cortisol. Our results suggest that HPA-mediated characteristics of social, cognitive, and emotional processes may exert an influence on a trait as complex as voting behavior, and that cortisol is a better predictor of actual voting behavior, as opposed to self-reported political activity.

  18. MR Imaging-derived Oxygen-Hemoglobin Dissociation Curves and Fetal-Placental Oxygen-Hemoglobin Affinities.

    Science.gov (United States)

    Avni, Reut; Golani, Ofra; Akselrod-Ballin, Ayelet; Cohen, Yonni; Biton, Inbal; Garbow, Joel R; Neeman, Michal

    2016-07-01

    Purpose To generate magnetic resonance (MR) imaging-derived, oxygen-hemoglobin dissociation curves and to map fetal-placental oxygen-hemoglobin affinity in pregnant mice noninvasively by combining blood oxygen level-dependent (BOLD) T2* and oxygen-weighted T1 contrast mechanisms under different respiration challenges. Materials and Methods All procedures were approved by the Weizmann Institutional Animal Care and Use Committee. Pregnant mice were analyzed with MR imaging at 9.4 T on embryonic days 14.5 (eight dams and 58 fetuses; imprinting control region ICR strain) and 17.5 (21 dams and 158 fetuses) under respiration challenges ranging from hyperoxia to hypoxia (10 levels of oxygenation, 100%-10%; total imaging time, 100 minutes). A shorter protocol with normoxia to hyperoxia was also performed (five levels of oxygenation, 20%-100%; total imaging time, 60 minutes). Fast spin-echo anatomic images were obtained, followed by sequential acquisition of three-dimensional gradient-echo T2*- and T1-weighted images. Automated registration was applied to align regions of interest of the entire placenta, fetal liver, and maternal liver. Results were compared by using a two-tailed unpaired Student t test. R1 and R2* values were derived for each tissue. MR imaging-based oxygen-hemoglobin dissociation curves were constructed by nonlinear least square fitting of 1 minus the change in R2*divided by R2*at baseline as a function of R1 to a sigmoid-shaped curve. The apparent P50 (oxygen tension at which hemoglobin is 50% saturated) value was derived from the curves, calculated as the R1 scaled value (x) at which the change in R2* divided by R2*at baseline scaled (y) equals 0.5. Results The apparent P50 values were significantly lower in fetal liver than in maternal liver for both gestation stages (day 14.5: 21% ± 5 [P = .04] and day 17.5: 41% ± 7 [P MR imaging-based oxygen-hemoglobin dissociation curves with a shorter protocol that excluded the hypoxic periods was demonstrated

  19. Baseline radioactivity levels of soil and grass in three selected sites

    International Nuclear Information System (INIS)

    Well documented baseline information is indispensable for emergency situations such as nuclear accidents, when an isotopic source is lost, or dealing with debris of reactor powered satellites. Maps of cosmic terrestrial natural and artificial radiation can be constructed by assembling different datasets such as ground and airborne gamma spectrometry, direct dose rate measurements and soil rock samples. In this study an attempt was made to set up baseline radioactivity concentration of Cs-137, U-238 and Th-232 (radionuclides) in soil and grass using gamma spectrometry. Continuous soil and grass sampling was done at two sites in Colombo and one site in Ranpokunugama Housing Scheme for a period of six months. The samples were analyzed for the above three radionuclides using gamma ray spectrometry. The activity concentration of Cs-137, U- 238 and Th- 232 in soil ranged from 0.6- 5.5 Bq per kg, 11.5- 34.9 Bq per kg and 6.4 to 34.2 Bq per kg respectively. The activity concentration of Cs-137 in grass ranged from 5.6- 12.4 Bq per kg

  20. Monthly continuous erythropoietin receptor activator treatment maintains stable hemoglobin levels in routine clinical management of hemodialysis patients.

    Science.gov (United States)

    Weinreich, Thomas; Leistikow, Frank; Hartmann, Hagen-Georg; Vollgraf, Günter; Dellanna, Frank

    2012-01-01

    Once-monthly administration of CERA, a continuous erythropoietin receptor activator, has shown equivalent efficacy to shorter-acting erythropoiesis-stimulating agents (ESAs) that require more frequent dosing, but data on routine use of once-monthly CERA in hemodialysis patients are lacking. Study on Efficacy, Safety and Applicability of Mircera (SESAM) was a prospective, multicenter, noninterventional trial with a duration of up to 9 months (month 0-5 "titration phase"; month 6-8 "evaluation phase") to test the stability of Hb control in hemodialysis patients under routine conditions. Patient selection, Hb targets and CERA dosing were at the discretion of the local nephrologist. 918 patients from 92 German nephrology centers were included. Ninety-three percent were on ESA treatment prior to study entry. The mean number of CERA dose changes during the study was 1.9 ± 1.9 per patient. Mean Hb level was 11.4 ± 1.2 g/dL at baseline and 11.7 ± 1.4 g/dL at the end of the 8-month study. During the evaluation phase (months 6-8), 15.6%, 40.3%, and 66.0% of patients had stable Hb (i.e., at least two values) in the ranges 11-12, 10-12, and 10-13 g/dL, respectively. The mean intra-individual fluctuation in Hb was 1.4 ± 0.7 g/dL during the study (0.5 ± 0.4 g/dL during the 3-month evaluation phase). More than 90% of patients, and > 80% of physicians, rated CERA therapy as "very good" or "good" throughout the study. Four patients (0.4%) discontinued prematurely due to adverse drug reactions. Once-monthly CERA therapy maintains stable Hb values with low intra-individual variability and few dose adaptations in hemodialysis patients when administered entirely according to local practice, and the regimen was well-tolerated.

  1. Effect of water deprivation on baseline and stress-induced corticosterone levels in the Children's python (Antaresia childreni).

    Science.gov (United States)

    Dupoué, Andréaz; Angelier, Frédéric; Lourdais, Olivier; Bonnet, Xavier; Brischoux, François

    2014-02-01

    Corticosterone (CORT) secretion is influenced by endogenous factors (e.g., physiological status) and environmental stressors (e.g., ambient temperature). Heretofore, the impact of water deprivation on CORT plasma levels has not been thoroughly investigated. However, both baseline CORT and stress-induced CORT are expected to respond to water deprivation not only because of hydric stress per se, but also because CORT is an important mineralocorticoid in vertebrates. We assessed the effects of water deprivation on baseline CORT and stress-induced CORT, in Children's pythons (Antaresia childreni), a species that experiences seasonal droughts in natural conditions. We imposed a 52-day water deprivation on a group of unfed Children's pythons (i.e., water-deprived treatment) and provided water ad libitum to another group (i.e., control treatment). We examined body mass variations throughout the experiment, and baseline CORT and stress-induced CORT at the end of the treatments. Relative body mass loss averaged ~10% in pythons without water, a value 2 to 4 times higher compared to control snakes. Following re-exposition to water, pythons from the water-deprived treatment drank readily and abundantly and attained a body mass similar to pythons from the control treatment. Together, these results suggest a substantial dehydration as a consequence of water deprivation. Interestingly, stress-induced but not baseline CORT level was significantly higher in water-deprived snakes, suggesting that baseline CORT might not respond to this degree of dehydration. Therefore, possible mineralocorticoid role of CORT needs to be clarified in snakes. Because dehydration usually induces adjustments (reduced movements, lowered body temperature) to limit water loss, and decreases locomotor performances, elevated stress-induced CORT in water-deprived snakes might therefore compensate for altered locomotor performances. Future studies should test this hypothesis.

  2. A novel methodology for the determination of biomarker baseline levels in the marine polychaete Hediste diversicolor.

    Science.gov (United States)

    Barrick, A; Châtel, A; Marion, J-M; Perrein-Ettajani, H; Bruneau, M; Mouneyrac, C

    2016-07-15

    Identifying environmental damage due to anthropogenic activities is a focal point for scientists and policy makers like those involved in the European Water Framework Directive (WFD). Many of these approaches focus on ecological endpoints for assessing environmental perturbations, which lead to policies emphasizing mitigation rather than prevention. Biomarkers provide early-warning indicators of stress but it is necessary to distinguish their natural variations from those induced by chemical stress. The global aim of this study was to establish a baseline assessment criterion (BAC) using historical data in a reference site to define toxicity thresholds. We have developed a multiple polynomial regression model (MPR) accounting the influence of salinity, temperature and size of individual on energetic reserves (glycogen and lipids) in the marine polychaete Hediste diversicolor. The model identified a complex, orthogonal relationship between confounding factors and glycogen and a linear relationship between lipids and size of individuals. PMID:27184131

  3. Gender Differences in Baseline Levels of Vascular Endothelial Growth Factor in the Plasma of Alaskan Sled Dogs

    Directory of Open Access Journals (Sweden)

    Scott W. Kemp

    2005-01-01

    Full Text Available Angiogenesis and vasculogenesis are two very important processes in the development and maintenance of mammalian health. In sled dogs, the angiogenic role is to supply and support tissue with ample vasculature, thus providing a route of access for the transportation of essential nutrients, including oxygen and the removal of waste in a sustained fashion. VEGF has been shown to be a key mediating factor in the underlying cascade of chemical events leading to angiogenesis, which makes it a very important precursor molecule for both muscle development and early neoplasia detection. The overall purpose of this study was to establish circulatory baseline VEGF levels in healthy dogs to develop an alternative mammalian model. Mean VEGF levels were 19 pg mL‾1 in sled dogs (n=20 and 5 pg mL‾1 in beagles (n=4. There were significant baseline VEGF-ir differences between male and female dogs and exercising males and exercising females. In addition preliminary data on beagles suggest that breed may play a role in baseline VEGF-ir levels. This study is the first step in the biotechnological development of a diagnostic VEGF assay in dogs.

  4. Estimation of serum, salivary immunoglobulin G, immunoglobulin A levels and total protein, hemoglobin in smokeless tobacco chewers and oral submucous fibrosis patients

    Directory of Open Access Journals (Sweden)

    Chandrakanth Balakrishnan

    2015-01-01

    Full Text Available Background: Oral submucous fibrosis (OSMF is a debilitating, potentially cancerous oral condition. Although areca nut is the most important causative agent, it is also considered that the disease is immunologically mediated. Aim of the Study: To establish that autoimmunity and nutritional deficiency play a role in the etiopathogenesis of OSMF. Objectives of the Study: To show that serum immunoglobulin markers (immunoglobulin-G [IgG], immunoglobulin-A [IgA] and nutritional parameters such as total serum protein (TSP, Hemoglobin (Hb play a role in causing OSMF and also to correlate serum, salivary IgG, IgA levels in OSMF patients. Settings and Design: A case-control study was done with 50 patients (25 patients who were provisionally diagnosed as OSMF - Group I, and 25 patients who were chronic smokeless tobacco chewers and who did not have any intraoral lesion - Group II. Materials and Methods: Five milliliters of blood and saliva were collected from both the groups. Quantitative analysis of serum, and salivary IgG, IgA was done by turbidometric immunoassay. TSP and Hemoglobin (Hb were estimated by spectrophotometry. Statistical Analysis: Results were analyzed by independent samples t-test and one-way analysis of variance (ANOVA. Results: All patients of OSMF showed significant (P < 0.01 increase in serum IgG, IgA, and salivary IgG levels as compared to smokeless tobacco chewers. The salivary IgA levels showed a significant decrease in OSMF patients (P < 0.05. TSP and Hb levels showed significant (P < 0.01 decrease in OSMF patients as compared to smokeless tobacco chewers. Conclusion: The elevation of immunoglobulin levels supports the concept of autoimmunity. The decrease in TSP and Hb suggests that nutritional deficiency plays a defined role in the occurrence as well as a further progression of OSMF.

  5. Sediment baseline study of levels and sources of polycyclic aromatic hydrocarbons and heavy metals in Lake Nicaragua

    DEFF Research Database (Denmark)

    Scheibye, Katrine; Weisser, Johan; Borggaard, Ole K.;

    2014-01-01

    Selected metals and polycyclic aromatic hydrocarbons (PAHs) were analyzed in sediment samples from 24 sites in Lake Nicaragua sampled May 2010 to provide a baseline of pollution levels. Cu exceeded the Consensus-Based Sediment Quality Guideline (CBSQG) Threshold Effect Concentrations (TECs) at 21...... showed that the CBSQG TECs were exceeded by naphthalene at five sites. The sum concentrations of the 16 US EPA priority PAHs (∑PAH16) ranged from 0.01mgkg(-1)dw to 0.64mgkg(-1)dw. The highest ∑PAH16 concentration was found upstream in River Mayales and the PAH composition revealed a heavy PAH fraction (e....... This study concluded that areas of Lake Nicaragua represent an important pollution baseline for future studies in this lake and other tropical lakes....

  6. Effects of Endurance Training on Lipid Metabolism and Glycosylated Hemoglobin Levels in Streptozotocin-induced Type 2 Diabetic Rats on a High-fat Diet.

    Science.gov (United States)

    Heo, Myoung; Kim, Eunjung

    2013-08-01

    [Purpose] Exercise has been recognized as a simple and economical therapeutic modality that effectively benefits patients with diabetes, for instance, increasing insulin sensitivity in type 2 diabetes. However, thus far, no studies have examined the effect of endurance training exercises on type 2 diabetes. Therefore, this study examined the effect of endurance training exercise regimens on body weight, glucose and insulin levels, lipid profiles, and HbA1c levels in STZ-induced type 2 diabetic rats on a high-fat diet. HbA1c was considered an indicator of glucose control during endurance training. [Methods] A total of 36 rats were included in this study. Diabetes was induced by administering STZ to 2 groups of 12 rats each, and, the remaining 12 rats were classified as the normal group. Biochemical parameters were measured 28 days later, and included: serum total cholesterol, triglyceride, high-density lipoprotein, glycosylated hemoglobin, glucose, and insulin levels. [Results] A significant decrease in serum TC and TG levels, and an increase in HDL cholesterol level were observed in the endurance training group. Moreover, blood glucose and HbA1c levels after 28 days of exercising were significantly lower in the endurance training group than in the control group (pdiet. We suggest that endurance training exercises may exhibit therapeutic, preventative, and protective effects against diabetes mellitus through improving lipid metabolism, glycemic control, and HbA1c levels.

  7. Hemoglobin Variants in Mice

    Energy Technology Data Exchange (ETDEWEB)

    Popp, Raymond A.

    1965-04-22

    Variability among mammalian hemoglobins was observed many years ago (35). The chemical basis for differences among hemoglobins from different species of mammals has been studied by several investigators (5, 11, 18, 48). As well as interspecies differences, hemoglobin variants are frequently found within a species of mammals (2, 3, 7, 16) The inheritance of these intraspecies variants can be studied, and pedigrees indicate that the type of hemoglobin synthesized in an individual is genetically controlled (20). Several of the variant human hemoglobins are f'unctionally deficient (7, 16). Such hemoglobin anomalies are of basic interest to man because of the vital role of hemoglobin for transporting oxygen to all tissues of the body.

  8. Individual variation in baseline and stress-induced corticosterone and prolactin levels predicts parental effort by nesting mourning doves

    Science.gov (United States)

    Miller, David A.; Vleck, Carol M.; Otis, David L.

    2009-01-01

    Endocrine systems have an important mechanistic role in structuring life-history trade-offs. During breeding, individual variation in prolactin (PRL) and corticosterone (CORT) levels affects behavioral and physiological processes that drive trade-offs between reproduction and self-maintenance. We examined patterns in baseline (BL) and stress induced (SI; level following a standard capture-restraint protocol) levels of PRL and CORT for breeding mourning doves (Zenaida macroura). We determined whether the relationship of adult condition and parental effort to hormone levels in wild birds was consistent with life-history predictions. Both BL PRL and BL CORT level in adults were positively related to nestling weight at early nestling ages, consistent with the prediction of a positive relationship of hormone levels to current parental effort of adults and associated increased energy demand. Results are consistent with the two hormones acting together at baseline levels to limit negative effects of CORT on reproduction while maintaining beneficial effects such as increased foraging for nestling feeding. Our data did not support predictions that SI responses would vary in response to nestling or adult condition. The magnitude of CORT response in the parents to our capture-restraint protocol was negatively correlated with subsequent parental effort. Average nestling weights for adults with the highest SI CORT response were on average 10–15% lighter than expected for their age in follow-up visits after the stress event. Our results demonstrated a relationship between individual hormone levels and within population variation in parental effort and suggested that hormonal control plays an important role in structuring reproductive decisions for mourning doves.

  9. Individual variation in baseline and stress-induced corticosterone and prolactin levels predicts parental effort by nesting mourning doves

    Science.gov (United States)

    Miller, David A.; Vleck, Carol M.; Otis, David L.

    2009-01-01

    Endocrine systems have an important mechanistic role in structuring life-history trade-offs. During breeding, individual variation in prolactin (PRL) and corticosterone (CORT) levels affects behavioral and physiological processes that drive trade-offs between reproduction and self-maintenance. We examined patterns in baseline (BL) and stress induced (SI; level following a standard capture-restraint protocol) levels of PRL and CORT for breeding mourning doves (Zenaida macroura). We determined whether the relationship of adult condition and parental effort to hormone levels in wild birds was consistent with life-history predictions. Both BL PRL and BL CORT level in adults were positively related to nestling weight at early nestling ages, consistent with the prediction of a positive relationship of hormone levels to current parental effort of adults and associated increased energy demand. Results are consistent with the two hormones acting together at baseline levels to limit negative effects of CORT on reproduction while maintaining beneficial effects such as increased foraging for nestling feeding. Our data did not support predictions that SI responses would vary in response to nestling or adult condition. The magnitude of CORT response in the parents to our capture-restraint protocol was negatively correlated with subsequent parental effort. Average nestling weights for adults with the highest SI CORT response were on average 10–15% lighter than expected for their age in follow-up visits after the stress event. Our results demonstrated a relationship between individual hormone levels and within population variation in parental effort and suggested that hormonal control plays an important role in structuring reproductive decisions for mourning doves.

  10. Led Astray by Hemoglobin A1c

    Directory of Open Access Journals (Sweden)

    Jean Chen MD

    2016-01-01

    Full Text Available Hemoglobin A1c (A1c is used frequently to diagnose and treat diabetes mellitus. Therefore, it is important be aware of factors that may interfere with the accuracy of A1c measurements. This is a case of a rare hemoglobin variant that falsely elevated a nondiabetic patient’s A1c level and led to a misdiagnosis of diabetes. A 67-year-old male presented to endocrine clinic for further management after he was diagnosed with diabetes based on an elevated A1c of 10.7%, which is approximately equivalent to an average blood glucose of 260 mg/dL. Multiple repeat A1c levels remained >10%, but his home fasting and random glucose monitoring ranged from 92 to 130 mg/dL. Hemoglobin electrophoresis and subsequent genetic analysis diagnosed the patient with hemoglobin Wayne, a rare hemoglobin variant. This variant falsely elevates A1c levels when A1c is measured using cation-exchange high-performance liquid chromatography. When the boronate affinity method was applied instead, the patient’s A1c level was actually 4.7%. Though hemoglobin Wayne is clinically silent, this patient was erroneously diagnosed with diabetes and started on an antiglycemic medication. Due to this misdiagnosis, the patient was at risk of escalation in his “diabetes management” and hypoglycemia. Therefore, it is important that providers are aware of factors that may result in hemoglobin A1c inaccuracy including hemoglobin variants.

  11. Baseline blood Pb levels of black-necked stilts on the upper Texas coast

    Science.gov (United States)

    Riecke, Thomas V.; Conway, Warren C.; Haukos, David A.; Moon, Jena A.; Comer, Christopher E.

    2015-01-01

    There are no known biological requirements for lead (Pb), and elevated Pb levels in birds can cause a variety of sub-lethal effects and mortality. Historic and current levels of Pb in mottled ducks (Anas fulvigula) suggest that environmental sources of Pb remain available on the upper Texas coast. Because of potential risks of Pb exposure among coexisting marsh birds, black-necked stilt (Himantopus mexicanus) blood Pb concentrations were measured during the breeding season. Almost 80 % (n = 120) of 152 sampled stilts exceeded the background threshold (>20 μg/dL) for Pb exposure. However, blood Pb concentrations did not vary by age or gender, and toxic or potentially lethal concentrations were rare (Pb concentrations of black-necked stilts in this study suggest the presence of readily bioavailable sources of Pb, although potential impacts on local stilt populations remain unclear.

  12. Increasing High-Level Waste Loading In Glass Without Changing The Baseline Melter Technology

    Energy Technology Data Exchange (ETDEWEB)

    Hrma, Pavel R.; Alton, Jesse; Plaisted, Trevor J.; Klouzek, Jaroslav; Matyas, Josef; Mika, Martin; Schill, Petr; Trochta, Miroslav; Nemec, Lubomir

    2001-02-25

    The main factors that determine the cost of high-level waste (HLW) vitrification are the waste loading (which determines the volume of glass) and the melting rate. Product quality should be the only factor determining the waste loading while melter design should provide a rapid melting technology. In reality, the current HLW melters are slow in glass-production rate and are subjected to operational risks that require waste loading to be kept far below its intrinsic level. One of the constraints that decrease waste loading is the liquidus-temperature limit. close inspection reveals that this constraint is probably too severe, even for the current technology. The purpose of the liquidus-temperature constraint is to prevent solids from settling on the melter bottom. It appears that some limited settling would niether interfere with melter operation nor shorten its lifetime and that the rate of settling can be greatly reduced if only small crystals are allowed to form.

  13. LHCb base-line level-0 trigger 3D-flow implementation

    CERN Document Server

    Crosetto, D

    1999-01-01

    The LHCb Level-0 trigger implementation with the 3D-Flow system offers full programmability, allowing it to adapt to unexpected operating conditions and enabling new, unpredicted physics. The implementation is described in detail and refers to components and technology available today. The 3D-Flow Processor system is a new, technology-independent concept in very fast, real-time system architectures. Based on the replication of a single type of circuit of 100 k gates, which communicates in six directions: bi-directional with North, East, West, and South neighbors, unidirectional from Top to Bottom, the system offers full programmability, modularity, ease of expansion and adaptation to the latest technology. A complete study of its applicability to the LHCb calorimeter triggers is presented. Full description of the input data handling, either in digital or mixed digital-analog form, of the data processing, and the transmission of results to the global level-0 trigger decision unit are provided. Any level-0 trig...

  14. Relationship Between Low Hemoglobin Levels and Outcomes After Treatment With Radiation or Chemoradiation in Patients With Cervical Cancer: Has the Impact of Anemia Been Overstated?

    Energy Technology Data Exchange (ETDEWEB)

    Bishop, Andrew J.; Allen, Pamela K.; Klopp, Ann H. [Department of Radiation Oncology, The University of Texas MD Anderson Cancer Center, Houston, Texas (United States); Meyer, Larissa A. [Department of Gynecologic Oncology and Reproductive Medicine, The University of Texas MD Anderson Cancer Center, Houston, Texas (United States); Eifel, Patricia J., E-mail: peifel@mdanderson.org [Department of Radiation Oncology, The University of Texas MD Anderson Cancer Center, Houston, Texas (United States)

    2015-01-01

    Purpose: Previous reports have suggested that anemia increases rates of recurrence after radiation therapy for cervical cancer. However, these studies may not have fully corrected for confounding risk factors. Using a well-characterized cohort of cervical cancer patients, we examined the association between anemia and outcomes before and after the introduction of chemoradiation as standard of care. Methods and Materials: We reviewed the records of 2454 patients who underwent definitive radiation therapy from 1980 through 2011. Minimum hemoglobin level (Hgb{sub min}) was recorded for 2359 patients (96%). Endpoints included freedom from central recurrence (FFCR), freedom from distant metastasis (FFDM), and disease-specific survival (DSS). Results: For the entire cohort, hemoglobin concentrations of 9, 10, and 12 g/dL before and during radiation were all significantly associated with FFCR, FFDM, and DSS (all P<.001) on univariate analysis. However, on multivariate analysis, only Hgb{sub min} less than 10 g/dL during RT (RT-Hgb{sub <10}) remained significant, and it was correlated with lower DSS (P=.02, hazard ratio [HR] = 1.28) and FFDM (P=.03, HR = 1.33) but not with FFCR. In a subset analysis of patients receiving chemoradiation (n=678), RT-Hgb{sub <10} was associated only with DSS (P=.008, HR = 1.49), not with FFCR or FFDM. In this subgroup, despite an association between RT-Hgb{sub <10} and DSS, the use of transfusion was not correlated with benefit. Conclusions: No evidence was found supporting anemia as an independent predictor of central recurrence in patients treated with definitive radiation therapy with or without chemotherapy. Less emphasis on correcting anemia in cervical cancer patients may be warranted.

  15. A common signaling pathway is activated in erythroid cells expressing high levels of fetal hemoglobin: a potential role for cAMP-elevating agents in β-globin disorders

    Directory of Open Access Journals (Sweden)

    Ikuta T

    2013-12-01

    Full Text Available Tohru Ikuta,1 Yuichi Kuroyanagi,1 Nadine Odo,1 Siyang Liu21Department of Anesthesiology and Perioperative Medicine, 2Department of Physiology, Medical College of Georgia, Georgia Regents University, Augusta, GA, USABackground: Although erythroid cells prepared from fetal liver, cord blood, or blood from β-thalassemia patients are known to express fetal hemoglobin at high levels, the underlying mechanisms remain elusive. We previously showed that cyclic nucleotides such as cAMP and cGMP induce fetal hemoglobin expression in primary erythroid cells. Here we report that cAMP signaling contributes to high-level fetal hemoglobin expression in erythroid cells prepared from cord blood and β-thalassemia.Methods: The status of the cAMP signaling pathway was investigated using primary erythroid cells prepared from cord blood and the mononuclear cells of patients with β-thalassemia; erythroid cells from adult bone marrow mononuclear cells served as the control.Results: We found that intracellular cAMP levels were higher in erythroid cells from cord blood and β-thalassemia than from adult bone marrow. Protein kinase A activity levels and cAMP-response element binding protein phosphorylation were higher in erythroid cells from cord blood or β-thalassemia than in adult bone marrow progenitors. Mitogen-activated protein kinase pathways, which play a role in fetal hemoglobin expression, were not consistently activated in cord blood or β-thalassemia erythroid cells. When cAMP signaling was activated in adult erythroid cells, fetal hemoglobin was induced at high levels and associated with reduced expression of BCL11A, a silencer of the β-globin gene.Conclusion: These results suggest that activated cAMP signaling may be a common mechanism among erythroid cells with high fetal hemoglobin levels, in part because of downregulation of BCL11A. Activation of the cAMP signaling pathway with cAMP-elevating agents may prove to be an important signaling mechanism to

  16. Effects of social environment on baseline glucocorticoid levels in a communally breeding rodent, the colonial tuco-tuco (Ctenomys sociabilis).

    Science.gov (United States)

    Woodruff, Julie A; Lacey, Eileen A; Bentley, George E; Kriegsfeld, Lance J

    2013-08-01

    The social environment in which an animal lives can profoundly impact its physiology, including glucocorticoid (GC) responses to external stressors. In social, group-living species, individuals may face stressors arising from regular interactions with conspecifics as well as those associated with basic life history needs such as acquiring food or shelter. To explore the relative contributions of these two types of stressors on glucocorticoid physiology in a communally breeding mammal, we characterized baseline GC levels in female colonial tuco-tucos (Ctenomys sociabilis), which are subterranean rodents endemic to southwestern Argentina. Long-term field studies have revealed that while about half of all yearling female C. sociabilis live and breed alone, the remainder live and breed within their natal group. We assessed the effects of this intraspecific variation in social environment on GC physiology by comparing concentrations of baseline fecal corticosterone metabolite (fCM) for (1) lone and group-living yearling females in a free-living population of C. sociabilis and (2) captive yearling female C. sociabilis that had been experimentally assigned to live alone or with conspecifics. In both cases, lone females displayed significantly higher mean baseline fCM concentrations. Data from free-living animals indicated that this outcome arose from differences in circadian patterns of GC production. fCM concentrations for group-living animals declined in the afternoon while fCM in lone individuals did not. These findings suggest that for C. sociabilis, stressors associated with basic life history functions present greater challenges than those arising from interactions with conspecifics. Our study is one of the first to examine GC levels in a plural-breeding mammal in which the effects of group-living are not confounded by differences in reproductive or dominance status, thereby generating important insights into the endocrine consequences of group-living.

  17. Baseline cardiac troponin t levels are elevated in subjects with untreated diabetes mellitus

    DEFF Research Database (Denmark)

    Pareek, M; Nielsen, M L; Leósdóttir, M;

    2015-01-01

    .26). After adjusting for age and sex, FPG category remained significantly predictive of hsTnT (B = 1.08 [95% confidence interval (CI), 0.56-1.59]; p cystatin C levels, and electrocardiographic left ventricular hypertrophy (LVH...... (B = 0.032 [95% CI, 0.012-0.051]; p = 0.001), and cystatin C (B = 3.69 [95% CI, 1.60-5.79]; p = 0.001). There was a significant interaction between FPG category and age (NFG: B= 0.22 [95% CI, 0.16-0.29]; IFG: B= 0.33 [95% CI, 0.18-0.48]; DM: B= 0.41 [95% CI, 0.20-0.62]; p = 0.03). Conclusions...

  18. Combination of 5-aminolevulinic acid and ferrous ion reduces plasma glucose and hemoglobin A1c levels in Zucker diabetic fatty rats.

    Science.gov (United States)

    Hara, Takeshi; Koda, Aya; Nozawa, Naoko; Ota, Urara; Kondo, Hikaru; Nakagawa, Hitoshi; Kamiya, Atsuko; Miyashita, Kazutoshi; Itoh, Hiroshi; Nakajima, Motowo; Tanaka, Tohru

    2016-06-01

    Mitochondrial dysfunction is associated with type 2 diabetes mellitus (T2DM). 5-Aminolevulinic acid (ALA), a natural amino acid produced only in the mitochondria, is a precursor of heme. Cytochromes that contain heme play an important role in aerobic energy metabolism. Thus, ALA may help reduce T2DM-associated hyperglycemia. In this study, we investigated the effect of ALA combined with sodium ferrous citrate (SFC) on hyperglycemia in Zucker diabetic fatty (ZDF) rats. We found that the gavage administration of ALA combined with SFC (ALA/SFC) for 6 weeks reduced plasma glucose and hemoglobin A1c (HbA1c) levels in rats without affecting plasma insulin levels. The glucose-lowering effect depended on the amount of ALA/SFC administered per day. Furthermore, the glucose tolerance was also significantly improved by ALA/SFC administration. Although food intake was slightly reduced in the rats administered ALA/SFC, there was no effect on their body weight. Importantly, ALA/SFC administration induced heme oxygenase-1 (HO-1) expression in white adipose tissue and liver, and the induced expression levels of HO-1 correlated with the glucose-lowering effects of ALA/SFC. Taken together, these results suggest that ALA combined with ferrous ion is effective in reducing hyperglycemia of T2DM without affecting plasma insulin levels. HO-1 induction may be involved in the mechanisms underlying the glucose-lowering effect of ALA/SFC. PMID:27239432

  19. Instantaneous Real-Time Kinematic Decimeter-Level Positioning with BeiDou Triple-Frequency Signals over Medium Baselines

    Directory of Open Access Journals (Sweden)

    Xiyang He

    2015-12-01

    Full Text Available Many applications, such as marine navigation, land vehicles location, etc., require real time precise positioning under medium or long baseline conditions. In this contribution, we develop a model of real-time kinematic decimeter-level positioning with BeiDou Navigation Satellite System (BDS triple-frequency signals over medium distances. The ambiguities of two extra-wide-lane (EWL combinations are fixed first, and then a wide lane (WL combination is reformed based on the two EWL combinations for positioning. Theoretical analysis and empirical analysis is given of the ambiguity fixing rate and the positioning accuracy of the presented method. The results indicate that the ambiguity fixing rate can be up to more than 98% when using BDS medium baseline observations, which is much higher than that of dual-frequency Hatch-Melbourne-Wübbena (HMW method. As for positioning accuracy, decimeter level accuracy can be achieved with this method, which is comparable to that of carrier-smoothed code differential positioning method. Signal interruption simulation experiment indicates that the proposed method can realize fast high-precision positioning whereas the carrier-smoothed code differential positioning method needs several hundreds of seconds for obtaining high precision results. We can conclude that a relatively high accuracy and high fixing rate can be achieved for triple-frequency WL method with single-epoch observations, displaying significant advantage comparing to traditional carrier-smoothed code differential positioning method.

  20. The Association between 25-Hydroxyvitamin D and Hemoglobin A1c Levels in Patients with Type 2 Diabetes and Stage 1–5 Chronic Kidney Disease

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    Farshad Kajbaf

    2014-01-01

    Full Text Available Aim. To examine the relationship between plasma 25-hydroxyvitamin D (25(OHD levels and blood hemoglobin A1c (HbA1c levels in diabetic patients at various stages of chronic kidney disease (CKD. Methods. We screened for data collected between 2003 and 2012. The correlation between 25(OHD and HbA1c levels was studied in patients categorized according to the severity of CKD and their vitamin D status. A multivariate linear regression model was used to determine whether 25(OHD and HbA1c levels were independently associated after adjustment for a number of covariates (including erythrocyte metformin levels. Results. We identified 542 reports from 245 patients. The mean HbA1c value was 6.7±1.0% in vitamin D sufficiency, 7.3±1.5% in insufficiency, and 8.4±2.0% in deficiency (P<0.0001. There was a negative correlation between 25(OHD and HbA1c levels for the population as a whole (r=-0.387, P<0.0001 and in the CKD severity subgroups (r=-0.384, P<0.0001 and r=-0.333, P<0.0001 for CKD stages 1–3 and 4-5, resp.. In the multivariate analysis, the 25(OHD level was the only factor associated with HbA1c (P<0.0001. Conclusion. 25(OHD levels were negatively correlated with HbA1c levels independently of study covariates.

  1. STUDY OF PHOSPHOROUS AND SULF HEMOGLOBIN LEVELS IN BLOOD AND ITS EFFECTS ON CBC OF MATCHE INDUSTRY WORKERS

    Directory of Open Access Journals (Sweden)

    SM. Karimian

    1999-12-01

    Full Text Available Blood sulfhemoglobin (S-Hb, total haemoglobin and phosphorous (P level and blood cell count in 33 matches industry workers of Azar Abhar, who were exposed directly or indirectly to chemical agents used in the factory (subjects, were measured. The results were compared with the result of the same measurement on 14 farm workers of the region (control and were analysed. In this study the effect of the work situation and duration of their occupation on the above-mentioned parameters were studied. The following results were obtained: S-Hb blood level between the subjects had a significant increase in respect to the control. This level in subjects of the regions with a high phosphorous pollution was higher than the other parts. Total haemoglobin concentration in the subjects yielded a significant decrease in respect to the control.

  2. The Folate-Vitamin B12 Interaction, Low Hemoglobin, and the Mortality Risk from Alzheimer's Disease.

    Science.gov (United States)

    Min, Jin-Young; Min, Kyoung-Bok

    2016-03-21

    Abnormal hemoglobin levels are a risk factor for Alzheimer's disease (AD). Although the mechanism underlying these associations is elusive, inadequate micronutrients, particularly folate and vitamin B12, may increase the risk for anemia, cognitive impairment, and AD. In this study, we investigated whether the nutritional status of folate and vitamin B12 is involved in the association between low hemoglobin levels and the risk of AD mortality. Data were obtained from the 1999-2006 National Health and Nutrition Examination Survey (NHANES) and the NHANES (1999-2006) Linked Mortality File. A total of 4,688 participants aged ≥60 years with available baseline data were included in this study. We categorized three groups based on the quartiles of folate and vitamin B12 as follows: Group I (low folate and vitamin B12); Group II (high folate and low vitamin B12 or low folate and high vitamin B12); and Group III (high folate and vitamin B12). Of 4,688 participants, 49 subjects died due to AD. After adjusting for age, sex, ethnicity, education, smoking history, body mass index, the presence of diabetes or hypertension, and dietary intake of iron, significant increases in the AD mortality were observed in Quartile1 for hemoglobin (HR: 8.4, 95% CI: 1.4-50.8), and the overall risk of AD mortality was significantly reduced with increases in the quartile of hemoglobin (p for trend = 0.0200), in subjects with low levels of both folate and vitamin B12 at baseline. This association did not exist in subjects with at least one high level of folate and vitamin B12. Our finding shows the relationship between folate and vitamin B12 levels with respect to the association between hemoglobin levels and AD mortality. PMID:27003215

  3. Impact of Hemoglobin A1c Levels on Residual Platelet Reactivity and Outcomes After Insertion of Coronary Drug-Eluting Stents (from the ADAPT-DES Study)

    DEFF Research Database (Denmark)

    Schoos, Mikkel M.; Dangas, George D.; Mehran, Roxana;

    2016-01-01

    . In conclusion, in this large-scale study, HbA1c and HPR were positively associated, but the clinical effect on adverse outcome was driven by poor glycemic control, which predicted ST and cardiac death after PCI regardless of PRU levels, warranting efforts to improve glycemic control after DES implantation......An increasing hemoglobin A1c (HbA1c) level portends an adverse cardiovascular prognosis; however, the association between glycemic control, platelet reactivity, and outcomes after percutaneous coronary intervention (PCI) with drug-eluting stents (DES) is unknown. We sought to investigate whether Hb...... >208 (42.5%, 50.2%, and 62.3%, p rates of definite or probable stent thrombosis (ST; 0.9%, 2.7%, and 4.2%, p = 0.02) increased progressively with HbA1c groups. Clinically relevant bleeding was greatest in the intermediate HbA1c group (8.2% vs 13.1% vs 9.5%, p = 0.04). In adjusted models...

  4. Accumulation and persistence of nicotine derived DNA and hemoglobin adducts in mice after multiple administration of {sup 14}C-nicotine at low dose level

    Energy Technology Data Exchange (ETDEWEB)

    Sun, Hongfang; Li, Hongli; Zhu, Jiadan; Wang, Haifang; Liu, Yuanfang [Peking Univ., Beijing (China). Dept. of Chemical Biology, College of Chemistry and Molecular Engineering; Liu, Kexin; Peng, Shixiang [Peking Univ., Beijing (China). Inst. of Heavy Ion Physics, School of Physics

    2004-07-01

    The hypothetic role of nicotine in causing smoking related diseases has not been well established. Based on our early finding of the genotoxicity of nicotine, a sub-chronic study on the accumulation and persistence of nicotine derived DNA and hemoglobin (Hb) adducts in mice following multiple low dose exposures was carried out by accelerator mass spectrometry (AMS). AMS is a sophisticated ultrasensitive nuclear method, which facilitates the detection of adduction of DNA and other bio-macromolecules with xenobiotics at human relevant environmental dose levels. Briefly, in this study [N-{sup 14}CH{sub 3}]-nicotine (s.a. 16.2 {mu}Ci/{mu}mol) was administered to mice by gavage once daily at 3.0 {mu}g/kg b.w., which is equivalent to an estimated nicotine dose inhaled by a 70 kg person smoking 5 cigarettes, for 14 consecutive days. Lung DNA, liver DNA and Hb were isolated from tissues and blood samples which were collected at 1, 3, 5, 7, 10, 14, 15, 17, 21 and 25 days time point, respectively. (orig.)

  5. Pre-Altitude Serum Ferritin Levels and Daily Oral Iron Supplement Dose Mediate Iron Parameter and Hemoglobin Mass Responses to Altitude Exposure.

    Directory of Open Access Journals (Sweden)

    Andrew D Govus

    Full Text Available To investigate the influence of daily oral iron supplementation on changes in hemoglobin mass (Hbmass and iron parameters after 2-4 weeks of moderate altitude exposure.Hematological data collected from 178 athletes (98 males, 80 females exposed to moderate altitude (1,350-3,000 m were analysed using linear regression to determine how altitude exposure combined with oral iron supplementation influenced Hbmass, total iron incorporation (TII and blood iron parameters [ferritin and transferrin saturation (TSAT].Altitude exposure (mean ± s: 21 ± 3 days increased Hbmass by 1.1% [-0.4, 2.6], 3.3% [1.7, 4.8], and 4.0% [2.0, 6.1] from pre-altitude levels in athletes who ingested nil, 105 mg and 210 mg respectively, of oral iron supplement daily. Serum ferritin levels decreased by -33.2% [-46.9, -15.9] and 13.8% [-32.2, 9.7] from pre-altitude levels in athletes who supplemented with nil and 105 mg of oral iron supplement daily, but increased by 36.8% [1.3, 84.8] in athletes supplemented with 210 mg of oral iron daily. Finally, athletes who ingested either 105 mg or 210 mg of oral iron supplement daily had a greater TII compared with non-supplemented athletes (0 versus 105 mg: effect size (d = -1.88 [-2.56, -1.17]; 0 versus 210 mg: effect size (d = -2.87 [-3.88, -1.66].Oral iron supplementation during 2-4 weeks of moderate altitude exposure may enhance Hbmass production and assist the maintenance of iron balance in some athletes with low pre-altitude iron stores.

  6. Evaluation of four novel genetic variants affecting hemoglobin A1c levels in a population-based type 2 diabetes cohort (the HUNT2 study

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    Platou Carl GP

    2011-02-01

    Full Text Available Abstract Background Chronic hyperglycemia confers increased risk for long-term diabetes-associated complications and repeated hemoglobin A1c (HbA1c measures are a widely used marker for glycemic control in diabetes treatment and follow-up. A recent genome-wide association study revealed four genetic loci, which were associated with HbA1c levels in adults with type 1 diabetes. We aimed to evaluate the effect of these loci on glycemic control in type 2 diabetes. Methods We genotyped 1,486 subjects with type 2 diabetes from a Norwegian population-based cohort (HUNT2 for single-nucleotide polymorphisms (SNPs located near the BNC2, SORCS1, GSC and WDR72 loci. Through regression models, we examined their effects on HbA1c and non-fasting glucose levels individually and in a combined genetic score model. Results No significant associations with HbA1c or glucose levels were found for the SORCS1, BNC2, GSC or WDR72 variants (all P-values > 0.05. Although the observed effects were non-significant and of much smaller magnitude than previously reported in type 1 diabetes, the SORCS1 risk variant showed a direction consistent with increased HbA1c and glucose levels, with an observed effect of 0.11% (P = 0.13 and 0.13 mmol/l (P = 0.43 increase per risk allele for HbA1c and glucose, respectively. In contrast, the WDR72 risk variant showed a borderline association with reduced HbA1c levels (β = -0.21, P = 0.06, and direction consistent with decreased glucose levels (β = -0.29, P = 0.29. The allele count model gave no evidence for a relationship between increasing number of risk alleles and increasing HbA1c levels (β = 0.04, P = 0.38. Conclusions The four recently reported SNPs affecting glycemic control in type 1 diabetes had no apparent effect on HbA1c in type 2 diabetes individually or by using a combined genetic score model. However, for the SORCS1 SNP, our findings do not rule out a possible relationship with HbA1c levels. Hence, further studies in other

  7. Involvement of Difference in Decrease of Hemoglobin Level in Poor Prognosis of Stage I and II Nasopharyngeal Carcinoma: Implication in Outcome of Radiotherapy

    International Nuclear Information System (INIS)

    Purpose: To investigate the effect of hemoglobin (Hb) concentration and the difference in its decrease during treatment on outcome of radiotherapy (RT) alone for patients with Stage I and II nasopharyngeal carcinoma. Methods and Materials: A total of 572 patients with Stage I-II nasopharyngeal carcinoma with RT alone between January 2001 and December 2004 were retrospectively analyzed. Patient characteristics, tumor variables, and Hb level, including pre-RT Hb, mid-RT Hb, and dynamic change of Hb between pre- and post- RT and its difference in decrease (▵Hb) were subjected to univariate and multivariable analysis to identify factors that predict disease-specific survival (DSS), local regional recurrence-free survival (LRFS), and metastases-free survival (MFS). Results: The 5-year DSS was poorer in the Hb continuous decrease group than in the Hb noncontinuous decrease group (84% vs. 89%; p = 0.008). There was poorer 5-year DSS in patients with ▵Hb of >11.5 g/L than in those with ▵Hb of ≤11.5 g/L (82% vs. 89%; p = 0.001), and poorer LRFS (79% vs. 83%; p = 0.035). Univariate and multivariate analysis showed that Hb decrease difference with greater than 11.5 g/L was an independent prognostic factor for DSS and LRFS. Conclusions: The difference in decrease of Hb level during the course of radiation treatment appeared as a poor prognostic factor in Stage I and II nasopharyngeal carcinoma patients.

  8. Role of altered esophageal intraluminal baseline impedance levels in patients with gatroesophageal reflux disease refractory to proton pump inhibitors.

    Science.gov (United States)

    Jiang, Liuqin; Ye, Bixing; Lin, Lin; Wang, Ying; Wang, Meifeng

    2016-08-01

    Numerous studies have investigated utility of esophageal intraluminal baseline impedance levels (BILs) in gastroesophageal reflux disease (GERD). However, effect of BILs in refractory GERD (RGERD) has not been well investigated. The aim of this study is to evaluate role of BILs in RGERD patients. Total 62 subjects with refractory gastroesophageal reflux symptoms underwent 24-hour impedance-pH monitoring and gastroendoscopy. Distal BILs in acid reflux type were significantly lower than those in nonacid reflux type and functional heartburn (FH) group. Distal BILs of reflux esophagitis (RE) patients were lower than those of nonerosive reflux disease (NERD) patients, while there were no statistical significance between 2 groups. Patients with severe esophagitis had lower distal BILs than those with mild esophagitis and NERD patients, and patients with severe esophagitis in acid reflux type had the lowest distal BILs. Distal BILs were significantly negatively correlated with DeMeester score, episodes of acid reflux, and acid exposure time, but no correlated with episodes of nonacid reflux. Characteristics of BILs in RGERD patients were similar with those in GERD patients, but might be more complicated. Evaluating BILs in RGERD patients could achieve a better understanding of pathophysiology in RGERD. PMID:27537561

  9. Phylogeny of Echinoderm Hemoglobins.

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    Ana B Christensen

    Full Text Available Recent genomic information has revealed that neuroglobin and cytoglobin are the two principal lineages of vertebrate hemoglobins, with the latter encompassing the familiar myoglobin and α-globin/β-globin tetramer hemoglobin, and several minor groups. In contrast, very little is known about hemoglobins in echinoderms, a phylum of exclusively marine organisms closely related to vertebrates, beyond the presence of coelomic hemoglobins in sea cucumbers and brittle stars. We identified about 50 hemoglobins in sea urchin, starfish and sea cucumber genomes and transcriptomes, and used Bayesian inference to carry out a molecular phylogenetic analysis of their relationship to vertebrate sequences, specifically, to assess the hypothesis that the neuroglobin and cytoglobin lineages are also present in echinoderms.The genome of the sea urchin Strongylocentrotus purpuratus encodes several hemoglobins, including a unique chimeric 14-domain globin, 2 androglobin isoforms and a unique single androglobin domain protein. Other strongylocentrotid genomes appear to have similar repertoires of globin genes. We carried out molecular phylogenetic analyses of 52 hemoglobins identified in sea urchin, brittle star and sea cucumber genomes and transcriptomes, using different multiple sequence alignment methods coupled with Bayesian and maximum likelihood approaches. The results demonstrate that there are two major globin lineages in echinoderms, which are related to the vertebrate neuroglobin and cytoglobin lineages. Furthermore, the brittle star and sea cucumber coelomic hemoglobins appear to have evolved independently from the cytoglobin lineage, similar to the evolution of erythroid oxygen binding globins in cyclostomes and vertebrates.The presence of echinoderm globins related to the vertebrate neuroglobin and cytoglobin lineages suggests that the split between neuroglobins and cytoglobins occurred in the deuterostome ancestor shared by echinoderms and vertebrates.

  10. The effect of seeing a family physician on the level of glycosylated hemoglobin (HbA1c in type 2 Diabetes Mellitus patients

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    Mahdavi Ali Reza

    2013-01-01

    Full Text Available Abstract Background Glycosylated hemoglobin (HbA1c in diabetic patients reflects the average blood glucose level, and will not be affected by variability in blood glucose in short time. Regular care of patients by medical staff could effectively control glycemic situation. The aim of this study was to assess the effect of medical care by general physicians on glycemic control by measuring of HbA1c. Methods In order to assess the effectiveness of National program for diabetes control and prevention in Iran, we compare HbA1c, Fasting blood glucose (FBS, systolic and diastolic blood pressure in two groups of diabetic patients diagnosed in this program. The first group consisted of patients who received at least four visits by General Physician (GP during one year after the diagnosis, and second group were patients who did not visited by GPs or received 1–3 visits. Results After one year, 24.1% of patients did not receive any care, while 57.9% examined at least once a year. Among visited patients, 23.5% received 1–3 times medical care and 23.5% received four or more visits. HbA1c was significantly lowered in patients with appropriate care (four and more compared with the non cared patients and patients with less than four cares. Conclusion Appropriate number of visits for each patient by GPs is an effective glycemic control in diabetic patients. Although this study provides a framework for medical care in diabetes, how to take care of these patients depends on specific situation of each patient and should be determined for each of them individually.

  11. Genome-wide association study identifies common loci influencing circulating glycated hemoglobin (HbA1c) levels in non-diabetic subjects

    DEFF Research Database (Denmark)

    An, Ping; Miljkovic, Iva; Thyagarajan, Bharat;

    2014-01-01

    Glycated hemoglobin (HbA1c) is a stable index of chronic glycemic status and hyperglycemia associated with progressive development of insulin resistance and frank diabetes. It is also associated with premature aging and increased mortality. To uncover novel loci for HbA1c that are associated...

  12. THE RENAL HANDLING OF HEMOGLOBIN

    Science.gov (United States)

    Bunn, H. Franklin; Esham, William T.; Bull, Robert W.

    1969-01-01

    The glomerular filtration of hemoglobin (α2β2) was studied under conditions in which its dissociation into αβ dimers was experimentally altered. Rats receiving hemoglobin treated with the sulfhydryl reagent bis(N-maleimidomethyl) ether (BME) showed a much lower renal excretion and prolonged plasma survival as compared with animals injected with untreated hemoglobin. Plasma disappearance was also prolonged in dogs receiving BME hemoglobin. Gel filtration data indicated that under physiological conditions, BME hemoglobin had impaired subunit dissociation. In addition, BME hemoglobin showed a very high oxygen affinity and a decreased rate of auto-oxidation. Glomerular filtration was enhanced under conditions which favor the dissociation of hemoglobin into dimers. Cat hemoglobin, which forms subunits much more extensively than canine hemoglobin, was excreted more readily by the rat kidney. The renal uptake of 59Fe hemoglobin injected intra-arterially into rabbits varied inversely with the concentration of the injected dose. PMID:5778789

  13. Variação dos níveis de hemoglobina de pacientes em hemodiálise tratados com eritropoetina: uma experiência brasileira Hemoglobin levels in hemodialysis patients treated with epoetin: a Brazilian experience

    Directory of Open Access Journals (Sweden)

    Adriano Luiz Ammirati

    2010-01-01

    Full Text Available OBJETIVO: Avaliar a variabilidade dos níveis de hemoglobina (Hb em pacientes em hemodiálise (HD tratados com eritropoetina. MÉTODOS: Foram coletados dados retrospectivos de 249 pacientes que estavam em HD e apresentavam, nos três meses anteriores, média de Hb entre 10,5 g/dL e 12,5 g/dL. O período de observação total foi de 36 meses. A cada mês de coleta, classificaram-se os valores de Hb em: 12,5g/dL. Além disto, os pacientes foram divididos em seis categorias de variabilidade da Hb: baixo persistente (12,5g/dL, flutuação de baixa amplitude com Hb baixo, flutuações de baixa amplitude com Hb alto e flutuações de alta amplitude. RESULTADOS: Mês a mês, a média da proporção de pacientes com Hb dentro da faixa alvo foi de 50% (variação, 42% a 61%. A proporção de valores de Hb médios acima da faixa alvo (30% foi mais frequente que a proporção abaixo do alvo (20%. Durante os períodos de seis, 12, e 36 meses, a proporção de pacientes com Hb baixa persistente se reduziu de 3,6% para 0%; de 31,7% para 2,8% naqueles com Hb alta persistente; de 7,6% para 0% naqueles com baixa amplitude com Hb baixo; e de 41,3% para 8,3% nos pacientes com baixa amplitude com Hb alto. Entretanto, houve aumento na proporção de pacientes (de 21,5% a 88,9% com alta amplitude de Hb. Portanto, à medida que o tempo de observação se alongou observou-se maior variabilidade dos valores de hemoglobina. Nenhum paciente manteve os níveis de Hb dentro do alvo durante todo o período do estudo. CONCLUSÃO: A manutenção da Hb dentro da faixa alvo é difícil, especialmente em períodos longos e a variabilidade ocorre mais frequentemente para valores mais elevados de Hb.OBJECTIVE: Correction of anemia using epoetin decreases morbidity and increases survival and quality of life in end-stage renal disease. Maintaining hemoglobin levels within the range proposed by guidelines has become a major challenge, with hemoglobin cycling affecting more than 90% of

  14. Optical Marking of Alcohol Induced Hemoglobin Modification

    CERN Document Server

    Vlokh, R; Moroz, O; Nastishin, Yu; Dudok, K; Dudok, T; Grinchishin, N; Nechiporenko, I; Hul, A

    2007-01-01

    It has been shown that conformational modifications of Hb induced by ethanol consumption can be visualized in optical spectra studying oxygenation kinetics of hemoglobin or mixing hemoglobin with Cibacron blue dye. Better dye affinity of blood proteins extracted from alcoholised rats with respect to those from non-alcoholised ones confirms that ethanol and its metabolites induce structural pathologies in blood protein molecules. The detected changes for the case of the posterity of intoxicated animals may be explained as a post-translation modification, as well as a disturbance of the structure and function of tissue cellular gene mechanism for the blood creation. It is established that alcohol intake during first four months leads to the decrease of fractional weight of oxyhemoglobin and to the increase of methemoglobin amount in blood. Further alcohol consumption is accompanied by recovering of the normal level of hemoglobin derivatives in blood. Normalization of the fractional weight of hemoglobin derivati...

  15. Prevalência de anemia e níveis séricos de hemoglobina em adolescentes segundo estágio de maturidade sexual Prevalence of anemia and hemoglobin serum levels in adolescents according to sexual maturation stage

    Directory of Open Access Journals (Sweden)

    Aline Brandão Mariath

    2006-12-01

    Full Text Available Durante a adolescência o crescimento acelerado promove um aumento nas necessidades nutricionais, inclusive de ferro, tornando os adolescentes um grupo de risco nutricional. O presente estudo objetivou avaliar a prevalência de anemia e os níveis séricos de hemoglobina de acordo com o estágio de maturidade sexual em uma amostra representativa composta por 272 adolescentes matriculados em escolas da rede municipal de ensino de Balneário Camboriú-SC. A auto-avaliação da maturidade sexual foi realizada segundo os critérios de Tanner (1962. Os níveis sangüíneos de hemoglobina foram medidos através do sistema HemoCue® e o diagnóstico de deficiência de ferro foi baseado no critério proposto pela Organização Mundial de Saúde (2001. Foram consideradas significativas as diferenças ao nível de pAccelerated growth spurt during adolescence leads to increased nutritional requirements, including iron, therefore posing a nutritional risk to adolescents. The present study aimed to assess the prevalence of anemia and hemoglobin serum levels according to the sexual maturation stage in a representative sample comprised of 272 adolescents enrolled in public schools in Balneário Camboriú, SC. Self-assessment of sexual maturation was carried out according to criteria defined by Tanner (1962. Hemoglobin serum levels were measured through the HemoCue® system and the diagnosis of iron deficiency was based on the cut off points proposed by the World Health Organization (2001. Differences were considered significant at the level of p<0.05. We found a prevalence of 31.2% of anemia without significant differences between genders. Hemoglobin serum levels were significantly higher in boys (p=0.044 than in girls. We did not find significant differences between mean hemoglobin levels of girls who had reached menarche and the ones who had not. There was no statistical association between the presence of anemia and sexual maturation stages of adolescents

  16. Methods for examination of antigenicity of heterogeneous polymerized hemoglobin

    International Nuclear Information System (INIS)

    Objective: To choose and establish the methods for examination of heterogeneous polymerized hemoglobin in order to offer the reference for evaluating the antigenicity of heterogeneous polymerized hemoglobin against human. Methods: Antigenicity of heterogeneous polymerized hemoglobin was examined for hypersensitivity, cell-mediated immunity reaction, humoral immunity reaction and cross-reaction of antigen. Results: The rabbit and guinea pig did not give rise to hypersensitivity. In immunized rabbits, the level of serum total IgG was normal, but the level of serum specific IgG was high. The examination of B lymphocytes showed that there was no significant difference (P>0.05) in comparison with control. Cross-reaction of antigen proved that bovine hemoglobin had cross-reaction with human hemoglobin. Suggesting that they may be homologous, the level of the serum specific antibody is high in the immunized animal. According to the immunology theories, the polymerized hemoglobin has antigenicity. (authors)

  17. Employing individual measures of baseline glucocorticoids as population-level conservation biomarkers: considering within-individual variation in a breeding passerine

    Science.gov (United States)

    Madliger, Christine L.; Love, Oliver P.

    2016-01-01

    Labile physiological variables, such as stress hormones [i.e. glucocorticoids (GCs)], allow individuals to react to perturbations in their environment and may therefore reflect the effect of disturbances or positive conservation initiatives in advance of population-level demographic measures. Although the application of GCs as conservation biomarkers has been of extensive interest, few studies have explicitly investigated whether baseline GC concentrations respond to disturbances consistently across individuals. However, confirmation of consistent responses is of paramount importance to assessing the ease of use of GCs in natural systems and to making valid interpretations regarding population-level change (or lack of change) in GC concentrations. We investigated whether free-ranging female tree swallows (Tachycineta bicolor) display individually specific changes in baseline glucocorticoid concentrations naturally over the breeding season (from incubation to offspring provisioning) and in response to a manipulation of foraging profitability (representing a decrease in access to food resources). We show that baseline GC concentrations are repeatable within individuals over reproduction in natural conditions. However, in response to a reduction in foraging ability, baseline GC concentrations increase at the population level but are not repeatable within individuals, indicating a high level of within-individual variation. Overall, we suggest that baseline GCs measured on a subset of individuals may not provide a representative indication of responses to environmental change at the population level, and multiple within-individual measures may be necessary to determine the fitness correlates of GC concentrations. Further validation should be completed across a variety of taxa and life-history stages. Moving beyond a traditional cross-sectional approach by incorporating repeated-measures methods will be necessary to assess the suitability of baseline GCs as biomarkers of

  18. Baseline participant characteristics and risk for dropout from ten obesity randomized controlled trials: a pooled analysis of individual level data

    Directory of Open Access Journals (Sweden)

    Kathryn Ann Kaiser

    2014-12-01

    Full Text Available Introduction: Understanding participant demographic characteristics that inform the optimal design of obesity RCTs have been examined in few studies. The objective of this study was to investigate the association of individual participant characteristics and dropout rates (DORs in obesity randomized controlled trials (RCT by pooling data from several publicly available datasets for analyses. We comprehensively characterize DORs and patterns in obesity RCTs at the individual study level, and describe how such rates and patterns vary as a function of individual-level characteristics. Methods: We obtained and analyzed nine publicly-available, obesity RCT datasets that examined weight loss or weight gain prevention as a primary or secondary endpoint. Four risk factors for dropout were examined by Cox proportional hazards including sex, age, baseline BMI, and race/ethnicity. The individual study data were pooled in the final analyses with a random effect for study, and HR and 95% CIs were computed. Results: Results of the multivariate analysis indicated that the risk of dropout was significantly higher for females compared to males (HR= 1.24, 95% CI = 1.05, 1.46. Hispanics and Non-Hispanic blacks had a significantly higher dropout rate compared to non-Hispanic whites (HR= 1.62, 95% CI = 1.37, 1.91; HR= 1.22, 95% CI = 1.11, 1.35, respectively. There was a significantly increased risk of dropout associated with advancing age (HR= 1.02, 95% CI = 1.01, 1.02 and increasing BMI (HR= 1.03, 95% CI = 1.03, 1.04. Conclusion/Significance: As more studies may focus on special populations, researchers designing obesity RCTs may wish to oversample in certain demographic groups if attempting to match comparison groups based on generalized estimates of expected dropout rates, or otherwise adjust a priori power estimates. Understanding true reasons for dropout may require additional methods of data gathering not generally employed in obesity RCTs, e.g. time on

  19. Hemoglobin level analysis in hemodialysis patients%维持血液透析患者血红蛋白达标情况分析

    Institute of Scientific and Technical Information of China (English)

    马晓莉; 丁巍; 孙晓丹; 李敏霞; 曹雪莹; 孙雪峰; 陈香美

    2012-01-01

    Objective To investigate the hemoglobin level in hemodialysis patients and its factors of influence. Methods Seventy-three hemodialysis patients were divided into group A( Hb≥l30 g/L ), B( 110 g/L≤Hb 0. 05 ). ⑤By univariate correlation analysis, Alb was positively correlated with Hb. However, EPO/HCT, β-MG, CRP and iPTH were negatively correlated with Hb ( P <0.05 ). ⑥By multivariate stepwise linear regression analysis, Hb level was strongly related to EPO/Hct, CRP, Alb, β - MG and iPTH. The standard regression coefficient ( Beta ) was - 1. 607, - 3. 391,0. 612 and - 1. 019 ,respectively. Conclusions The low response for r-HuEPO is the main reason for non-compliance for anemia treatment. The level of micro-inflammation, nutritional status and inadequate removal of toxins may be the risk factors.%目的 了解维持血液透析患者贫血治疗达标情况并分析其影响因素.方法 选择我院规律血液透析患者73例.按血红蛋白(hemoglobin,Hb)水平将患者分为3组:A组为Hb≥130 g/L患者;B组为110 g/L≤ Hb<130 g/L患者;C组为Hb<110 g/L患者.透析前采血检测肾功能、血常规、电解质、白蛋白(albumin,Alb)、C反应蛋白(C-reactive protein,CRP)、β2-微球蛋白(β2-microglobulin,β2-MG)、血清全段甲状旁腺激素(intact parathyroid hormone,iPTH)、血清铁蛋白(serum ferritin,SF)等参数.记录最近4周重组人红细胞生成素(recombinant human erythropoietin,r-HuEPO)的应用总量,以每周应用总量同红细胞压积(hematocrit,Hct)的比值(r-HuEPO dosage to Hct,EPO/Hct)作为评价r-HuEPO低反应的指标.比较3组患者上述指标的差别,分析各因素与Hb的相关性.结果①73例患者中,Hb≥110 g/L者62例,占全部患者的84.93%.②EPO/Hct比值,B组患者明显高于A组患者(P<0.05).③C组患者EPO/Hct比值、CRP、β2-MG及iPTH水平均高于A组和B组(P<0.05).④其余各项指标三组间差异无统计学意义(P>0.05).⑤单因素相关

  20. Comparison between the Strength Levels of Baseline Nuclear-Grade Graphite and Graphite Irradiated in AGC-2

    Energy Technology Data Exchange (ETDEWEB)

    Carroll, Mark Christopher [Idaho National Laboratory (INL), Idaho Falls, ID (United States)

    2015-07-01

    This report details the initial comparison of mechanical strength properties between the cylindrical nuclear-grade graphite specimens irradiated in the second Advanced Graphite Creep (AGC-2) experiment with the established baseline, or unirradiated, mechanical properties compiled in the Baseline Graphite Characterization program. The overall comparative analysis will describe the development of an appropriate test protocol for irradiated specimens, the execution of the mechanical tests on the AGC-2 sample population, and will further discuss the data in terms of developing an accurate irradiated property distribution in the limited amount of irradiated data by leveraging the considerably larger property datasets being captured in the Baseline Graphite Characterization program. Integrating information on the inherent variability in nuclear-grade graphite with more complete datasets is one of the goals of the VHTR Graphite Materials program. Between “sister” specimens, or specimens with the same geometry machined from the same sub-block of graphite from which the irradiated AGC specimens were extracted, and the Baseline datasets, a comprehensive body of data will exist that can provide both a direct and indirect indication of the full irradiated property distributions that can be expected of irradiated nuclear-grade graphite while in service in a VHTR system. While the most critical data will remain the actual irradiated property measurements, expansion of this data into accurate distributions based on the inherent variability in graphite properties will be a crucial step in qualifying graphite for nuclear use as a structural material in a VHTR environment.

  1. Comparison of glycosylated hemoglobin levels detected by 3 kinds of analytic instruments%3种仪器检测糖化血红蛋白结果分析

    Institute of Scientific and Technical Information of China (English)

    张卫军; 黄胜起; 骆连妹; 李秀娟; 郑艳芬

    2014-01-01

    Objective To compare levels of glycosylated hemoglobin detected by 3 kinds of analytic instruments .Methods 75 samples were measured by D-10 glycosylated hemoglobin automatic analyzer(ionexchange chromatography) ,HA-8160 glycosylated hemoglobin automatic analyzer(affinity chromatography) and 7170A automatic analyzer(immune turbidimetry) .Results were tested by the homogeneity of variance ,the one-way analysis of variance and correlation analysis .Results There was a positive correlation between D-10 glycosylated hemoglobin automatic analyzer and HA-8160 glycosylated hemoglobin automatic analyzer(r2 =0 .996) , the linear equation was Y=0 .953X+0 .519 .There was a positive correlation between D-10 glycosylated hemoglobin automatic ana-lyzer and 7170A automatic analyzer(r2 =0 .996) ,the linear equation was Y=0 .925X+0 .576 .There was a positive correlation be-tween HA-8160 glycosylated hemoglobin automatic analyzer and 7170A automatic analyzer(r2 =0 .998) ,the linear equation was Y=0 .969 X+0 .081 .Conclusion In the premise of quality control in laboratory ,three different instrument can use to detect the level of glycosylated hemoglobin .%目的:比较3种仪器检测糖化血红蛋白的结果。方法分别采用D-10全自动糖化血红蛋白分析仪(离子交换色谱法)、HA-8160全自动糖化血红蛋白分析仪(亲和色谱法)和7170A全自动生化分析仪(免疫比浊法)检测75例全血样本,对检测结果进行方差齐性检验,方差齐后,进行单因素方差分析和相关性分析。结果 D-10全自动糖化血红蛋白分析仪和 H A-8160全自动糖化血红蛋白分析仪测定结果呈正相关( r2=0.996),回归方程为Y =0.953 X+0.519;D-10全自动糖化血红蛋白分析仪和7170A全自动生化分析仪测定结果呈正相关(r2=0.996),回归方程为Y =0.925 X+0.576;HA-8160全自动糖化血红蛋白分析仪和7170A全自动生化分析仪测定结果呈正相关(r2

  2. Baseline rationing

    DEFF Research Database (Denmark)

    Hougaard, Jens Leth; Moreno-Ternero, Juan D.; Østerdal, Lars Peter Raahave

    The standard problem of adjudicating conflicting claims describes a situation in which a given amount of a divisible good has to be allocated among agents who hold claims against it exceeding the available amount. This paper considers more general rationing problems in which, in addition to claims......, there exist baselines (to be interpreted as objective entitlements, ideal targets, or past consumption) that might play an important role in the allocation process. The model we present is able to accommodate real-life rationing situations, ranging from resource allocation in the public health care sector...... to international protocols for the reduction of greenhouse emissions, or water distribution in drought periods. We define a family of allocation methods for such general rationing problems - called baseline rationing rules - and provide an axiomatic characterization for it. Any baseline rationing rule within...

  3. Baseline Serum Lactate Dehydrogenase Levels for Patients Treated With Intensity-Modulated Radiotherapy for Nasopharyngeal Carcinoma: A Predictor of Poor Prognosis and Subsequent Liver Metastasis

    International Nuclear Information System (INIS)

    Purpose: To evaluate the prognostic value of baseline serum lactate dehydrogenase (LDH) levels in patients with nasopharyngeal carcinoma (NPC) treated with intensity-modulated radiotherapy (IMRT). Methods and Materials: Cases of NPC (n = 465) that involved treatment with IMRT with or without chemotherapy were retrospectively analyzed. Results: The mean (±SD) and median baseline serum LDH levels for this cohort were 172.77 ± 2.28 and 164.00 IU/L, respectively. Levels of LDH were significantly elevated in patients with locoregionally advanced disease (p = 0.016). Elevated LDH levels were identified as a prognostic factor for rates of overall survival (OS), disease-free survival (DFS), and distant metastasis-free survival (DMFS), with p values <0.001 in the univariate analysis and p < 0.001, p = 0.004, and p = 0.003, respectively, in the multivariate analysis. Correspondingly, the prognostic impact of patient LDH levels was found to be statistically significant for rates of OS, DFS, and DMFS (p = 0.028, 0.024, and 0.020, respectively). For patients who experienced subsequent liver failure after treatment, markedly higher pretreatment serum LDH levels were detected compared with patients experiencing distant metastasis events at other sites (p = 0.032). Conclusions: Elevated baseline LDH levels are associated with clinically advanced disease and are a poor prognosticator for OS, DFS, and DMFS for NPC patients. These results suggest that elevated serum levels of LDH should be considered when evaluating treatment options.

  4. Immunohistochemical detection of osteopontin in advanced head-and-neck cancer: Prognostic role and correlation with oxygen electrode measurements, hypoxia-inducible-factor-1α-related markers, and hemoglobin levels

    International Nuclear Information System (INIS)

    Purpose: The tumor-associated glycoprotein osteopontin (OPN) is discussed as a plasma marker of tumor hypoxia. However, the association of immunohistochemical OPN expression in tumor sections with tumor oxygenation parameters (HF5, median pO2), the hypoxia-related markers hypoxia-inducible factor-1α (HIF-1α) and carbonic anhydrase IX (CAIX), or hemoglobin and systemic vascular endothelial growth factor (VEGF) levels has not been investigated. Methods and Materials: Tumor tissue sections of 34 patients with advanced head-and-neck cancer treated with radiotherapy were assessed by immunochemistry for the expression of OPN, HIF-1α, and CA IX. Relationship of OPN expression with tumor oxygenation parameters (HF5, median pO2), HIF-1α and CA IX expression, hemoglobin and serum VEGF level, and clinical parameters was studied. Results: Bivariate analysis showed a significant correlation of positive OPN staining with low hemoglobin level (p = 0.02), high HIF-1α expression (p = 0.02), and high serum vascular endothelial growth factor level (p = 0.02) for advanced head-and-neck cancer. Furthermore, considering the 31 Stage IV patients, the median pO2 correlated significantly with the OPN expression (p = 0.02). OPN expression alone had only a small impact on prognosis. However, in a univariate Cox proportional hazard regression model, the expression of either OPN or HIF-1α or CA IX was associated with a 4.1-fold increased risk of death (p = 0.02) compared with negativity of all three markers. Conclusion: Osteopontin expression detected immunohistochemically is associated with oxygenation parameters in advanced head-and-neck cancer. When the results of OPN, HIF-1α, and CA IX immunohistochemistry are combined into a hypoxic profile, a strong and statistically significant impact on overall survival is found

  5. Acrylamide and glycidamide hemoglobin adduct levels and endometrial cancer risk: A nested case-control study in nonsmoking postmenopausal women from the EPIC cohort.

    Science.gov (United States)

    Obón-Santacana, Mireia; Freisling, Heinz; Peeters, Petra H; Lujan-Barroso, Leila; Ferrari, Pietro; Boutron-Ruault, Marie-Christine; Mesrine, Sylvie; Baglietto, Laura; Turzanski-Fortner, Renee; Katzke, Verena A; Boeing, Heiner; Quirós, J Ramón; Molina-Portillo, Elena; Larrañaga, Nerea; Chirlaque, María-Dolores; Barricarte, Aurelio; Khaw, Kay-Tee; Wareham, Nick; Travis, Ruth C; Merritt, Melissa A; Gunter, Marc J; Trichopoulou, Antonia; Lagiou, Pagona; Naska, Androniki; Palli, Domenico; Sieri, Sabina; Tumino, Rosario; Fiano, Valentina; Galassom, Rocco; Bueno-de-Mesquita, H B As; Onland-Moret, N Charlotte; Idahl, Annika; Lundin, Eva; Weiderpass, Elisabete; Vesper, Hubert; Riboli, Elio; Duell, Eric J

    2016-03-01

    Acrylamide, classified in 1994 by IARC as "probably carcinogenic to humans," was discovered in 2002 in some heat-treated, carbohydrate-rich foods. Four prospective studies have evaluated the association between dietary acrylamide intake and endometrial cancer (EC) risk with inconsistent results. The purpose of this nested case-control study, based on the European Prospective Investigation into Cancer and Nutrition (EPIC) cohort, was to evaluate, for the first time, the association between hemoglobin adducts of acrylamide (HbAA) and glycidamide (HbGA) and the risk of developing EC in non-smoking postmenopausal women. Hemoglobin adducts were measured in red blood cells by HPLC/MS/MS. Four exposure variables were evaluated: HbAA, HbGA, their sum (HbAA+HbGA), and their ratio (HbGA/HbAA). The association between hemoglobin adducts and EC was evaluated using unconditional multivariable logistic regression models, and included 383 EC cases (171 were type-I EC), and 385 controls. Exposure variables were analyzed in quintiles based on control distributions. None of the biomarker variables had an effect on overall EC (HRHbAA;Q5vsQ1 : 0.84, 95%CI: 0.49-1.48; HRHbGA;Q5vsQ1 : 0.94, 95%CI: 0.54-1.63) or type-I EC risk. Additionally, none of the subgroups investigated (BMI acrylamide or glycidamide were not associated with EC or type-I EC risk in 768 nonsmoking postmenopausal women from the EPIC cohort.

  6. Antimicrobial properties of hemoglobin.

    Science.gov (United States)

    Sheshadri, Preethi; Abraham, Jayanthi

    2012-12-01

    Hemoglobin consists of a heme containing component and a globin unit. It exists as a tetramer with 2 α subunits and 2 β subunits in adults and with 2 α subunits and 2 γ chains in infants. On proteolytic cleavage, hemoglobin breaks down to produce many biologically active compounds, among which are hemocidins, those which exhibit antimicrobial property. The generation of these peptides does not depend on the blood group, Rhesus factor, age and sex of the healthy donors. The microbicidal activity has been observed against a variety of gram positive and Gram-negative bacteria, and against filamentous fungi, yeast and even certain parasites. The discovery of hemocidins opens a new field for research into the details of the peptides acting as second line of defence in boosting the innate immune system of the organisms.

  7. Hemoglobin oxidative stress

    Energy Technology Data Exchange (ETDEWEB)

    Croci, S.; Ortalli, I.; Pedrazzi, G. [University of Parma, Istituto di Scienze Fisiche, INFM-Udr Parma (Italy); Passeri, G. [University of Parma, Dipartimento di Medicina Interna e Scienze Biomediche (Italy); Piccolo, P. [University of Parma, Istituto di Clinica chirurgica Generale, Toracica e Vascolare (Italy)

    2000-07-15

    Venous blood obtained from healthy donors and from patients suffering from breast cancer have been treated with acetylphenylhydrazine (APH) for different time. Moessbauer spectra of the packed red cells have been recorded and compared. The largest difference occurs after 50 min of treatment with APH where the patient samples show a broad spectral pattern indicating an advanced hemoglobin oxidation. These results may have some relevance in early cancer diagnosis.

  8. Plant hemoglobins: Important players at the crossroads between oxygen and nitric oxide

    DEFF Research Database (Denmark)

    Gupta, Kapuganti J; Hebelstrup, Kim; Mur, Luis A J;

    2011-01-01

    Plant hemoglobins constitute a diverse group of hemeproteins and evolutionarily belong to three different classes. Class 1 hemoglobins possess an extremely high affinity to oxygen and their main function consists in scavenging of nitric oxide (NO) at very low oxygen levels. Class 2 hemoglobins have...... a lower oxygen affinity and they facilitate oxygen supply to developing tissues. Symbiotic hemoglobins in nodules have mostly evolved from class 2 hemoglobins. Class 3 hemoglobins are truncated and represent a clade with a very low similarity to class 1 and 2 hemoglobins. They may regulate oxygen delivery...... at high O2 concentrations. Depending on their physical properties, hemoglobins belong either to hexacoordinate non-symbiotic or pentacoordinate symbiotic groups. Plant hemoglobins are plausible targets for improving resistance to multiple stresses....

  9. Symbiotic and nonsymbiotic hemoglobin genes of Casuarina glauca

    DEFF Research Database (Denmark)

    Jacobsen-Lyon, K; Jensen, Erik Østergaard; Jørgensen, Jan-Elo;

    1995-01-01

    Casuarina glauca has a gene encoding hemoglobin (cashb-nonsym). This gene is expressed in a number of plant tissues. Casuarina also has a second family of hemoglobin genes (cashb-sym) expressed at a high level in the nodules that Casuarina forms in a nitrogen-fixing symbiosis with the actinomycete...

  10. Double blind randomized placebo-controlled trial on the effects of testosterone supplementation in elderly men with moderate to low testosterone levels: design and baseline characteristics [ISRCTN23688581

    Directory of Open Access Journals (Sweden)

    Verhaar Harald JJ

    2006-08-01

    Full Text Available Abstract In ageing men testosterone levels decline, while cognitive function, muscle and bone mass, sexual hair growth, libido and sexual activity decline and the risk of cardiovascular diseases increase. We set up a double-blind, randomized placebo-controlled trial to investigate the effects of testosterone supplementation on functional mobility, quality of life, body composition, cognitive function, vascular function and risk factors, and bone mineral density in older hypogonadal men. We recruited 237 men with serum testosterone levels below 13.7 nmol/L and ages 60–80 years. They were randomized to either four capsules of 40 mg testosterone undecanoate (TU or placebo daily for 26 weeks. Primary endpoints are functional mobility and quality of life. Secondary endpoints are body composition, cognitive function, aortic stiffness and cardiovascular risk factors and bone mineral density. Effects on prostate, liver and hematological parameters will be studied with respect to safety. Measure of effect will be the difference in change from baseline visit to final visit between TU and placebo. We will study whether the effect of TU differs across subgroups of baseline waist girth ( At baseline, mean age, BMI and testosterone levels were 67 years, 27 kg/m2 and 10.72 nmol/L, respectively.

  11. Low Baseline Interleukin-17A Levels Are Associated with Better Treatment Response at 12 Weeks to Tocilizumab Therapy in Rheumatoid Arthritis Patients

    Directory of Open Access Journals (Sweden)

    Sang Jin Lee

    2015-01-01

    Full Text Available T helper 17-related cytokines have been implicated in rheumatoid arthritis (RA pathogenesis. The study aimed to identify cytokines associated with the treatment response of RA patients to tocilizumab (TCZ, a humanized monoclonal antibody against the interleukin- (IL- 6 receptor. As an independent substudy of the 24-week, randomized, double-blinded CWP-TCZ301 trial of TCZ in RA patients with an inadequate response to disease-modifying antirheumatic drugs, serum levels of cytokines including tumor necrosis factor-alpha, IL-17A, IL-21, IL-23, IL-6, and soluble IL-6 receptor were measured. Baseline IL-17A levels were significantly lower in RA patients who achieved disease activity score 28 (DAS28 remission at 12 weeks of TCZ treatment, compared to patients not in remission. Patients were stratified into IL-17A low group and IL-17A high group. Significantly more patients in the IL-17A low group achieved remission as compared to the IL-17A high group (47.6 versus 17.4%, P=0.032. DAS28 improvement was significantly better in the IL-17A low group than in the IL-17A high group at 12 weeks (P=0.045 and 24 weeks (P=0.046 after adjustment. Other baseline cytokines were not associated with treatment response to TCZ. The data demonstrate that low baseline IL-17A levels are associated with better clinical response to TCZ treatment in RA patients.

  12. Anemia intrahospitalaria y descenso de hemoglobina en pacientes internados Hospital-acquired anemia and decrease of hemoglobin levels in hospitalized patients

    Directory of Open Access Journals (Sweden)

    Carina V. Gianserra

    2011-06-01

    estadía hospitalaria prolongada, la presencia de leucocitosis, la hidratación parenteral y colocación de acceso venoso central fueron factores predictivos de descenso de hemoglobina = 2 g/dl.It is common to observe the development of anemia in hospitalized patients, especially in critical cases. Few studies have evaluated its prevalence and associated factors in patients in the general ward. The purpose of this study is to determine the prevalence, characteristics and associated clinical factors of hospital-acquired anemia and the drop of hemoglobin concentration in hospitalized patients. This is a cross-sectional, prospective and descriptive study. A total of 192 consecutive in-patients in the general ward were studied. Associated risk factors to the drop in hemoglobin by = 2g/dl were analyzed; 139 patients (72.4% presented anemia; 89 of them (46.4% had it at admission and 50 (26% developed hospital-acquired anemia, 47 out of 192 showed a drop in hemoglobin = 2 g/dl(24.48%. They also presented lower values of hematocrite and hemoglobin at discharge (p = 0.01, parenteral hydration at a higher volume (p = 0.01, and lengthier hospitalizations (p = 0.0001. In the univariate analysis, the following variables were statistically significant risk factors: leukocytosis = 11 000 mm³ (OR; IC95%: 2,02; 1.03-4; p = 0.01, hospitalization days = 7 (OR; IC95%:3.39; 1.62-7.09; p = 0.0006, parenteral hydration = 1500 ml/day (OR; IC95%: 2.47; 1.06-6.4; p = 0.01, central venous access (OR; IC95%:10.29; 1.75-108.07; p = 0.003 and hospital-acquired anemia (OR; IC95%: 7.06; 3.41-15.83; p = 0.00000004. In the multivariate analysis, the following variables were independent predictive factors of the hemoglobin decrease = 2 g/dl: leukocytosis = 11 000 mm³ (OR; IC95%: 2.45; 1.14-5,27; p = 0.02, hospitalization days = 7 (OR; IC95%:5.15; 2.19-12.07; p = 0.0002, parenteral hydration = 1500 ml/day (OR; IC95%: 2.95; 1.13-7.72; p = 0.02, central venous access (OR; IC95%:8.82; 1.37-56.82; p = 0

  13. Predictors of hemoglobin in Danish blood donors

    DEFF Research Database (Denmark)

    Kotzé, Sebastian R; Pedersen, Ole B; Petersen, Mikkel S;

    2015-01-01

    BACKGROUND: It is well known that blood donors are at increased risk of iron deficiency and subsequent development of iron deficiency anemia. We aimed to investigate the effect of factors influencing hemoglobin (Hb) levels. STUDY DESIGN AND METHODS: Initiated in 2010, the Danish Blood Donor Study...

  14. Ground level measurement of nuclei from coal development in the northern Great Plains: baseline measurements. Final report

    Energy Technology Data Exchange (ETDEWEB)

    Davis, B. L.; Johnson, L. R.; Sengupta, S.; Yue, P. C.

    1978-11-01

    The Institute of Atmospheric Sciences of the South Dakota School of Mines and Technology has completed 20 months of ambient air sampling at rural and remote sites in a five-state region of the northern Great Plains. Sampling was accomplished by use of a 27-ft motor home laboratory containing living accommodations for a field crew of two. The laboratory was outfitted with a number of instruments for measurement of pollutant parameters: cloud condensation nuclei, ice nuclei, Aitken nuclei, size distribution information for Aitken size particulate, sulfur dioxide, ozone, raindrop size distributions, and pH of precipitation. In addition, an instrumented meteorological tower provided wind speed, wind direction, ambient air temperature, and dew-point temperature. Instruments varied as to durability and success of operation, but better than 90% data retrieval was possible for the entire 20-month sampling study. Analyses of the large quantities of data obtained were not possible under the initial baseline measurement program, but examination of most parameters indicate that the air masses in the northern Great Plains are still relatively clean and are influenced primarily by local sources of contamination rather than large regional sources. Particulate concentrations in these remote areas are representative of mountain stations or clean rural conditions, and sulfur dioxide concentrations are at the threshold of detectability of the instrument. Precipitation is only very slightly acidic, and no significant quantity of amorphous particles (such as coal dust or combustion products) is found in the quantitative analyses of the high-volume filter collections. A summary of ''average'' conditions observed over the study area is tabulated.

  15. Comparison of Toxicities to Vibrio fischeri and Fish Based on Discrimination of Excess Toxicity from Baseline Level.

    Science.gov (United States)

    Wang, Xiao H; Yu, Yang; Huang, Tao; Qin, Wei C; Su, Li M; Zhao, Yuan H

    2016-01-01

    Investigations on the relationship of toxicities between species play an important role in the understanding of toxic mechanisms to environmental organisms. In this paper, the toxicity data of 949 chemicals to fish and 1470 chemicals to V. fischeri were used to investigate the modes of action (MOAs) between species. The results show that although there is a positive interspecies correlation, the relationship is poor. Analysis on the excess toxicity calculated from toxic ratios (TR) shows that many chemicals have close toxicities and share the same MOAs between the two species. Linear relationships between the toxicities and octanol/water partition coefficient (log KOW) for baseline and less inert compounds indicate that the internal critical concentrations (CBRs) approach a constant both to fish and V. fischeri for neutral hydrophobic compounds. These compounds share the same toxic mechanisms and bio-uptake processes between species. On the other hand, some hydrophilic compounds exhibit different toxic effects with greatly different log TR values between V. fischeri and fish species. These hydrophilic compounds were identified as reactive MOAs to V. fischeri, but not to fish. The interspecies correlation is improved by adding a hydrophobic descriptor into the correlation equation. This indicates that the differences in the toxic ratios between fish and V. fischeri for these hydrophilic compounds can be partly attributed to the differences of bioconcentration between the two species, rather than the differences of reactivity with the target macromolecules. These hydrophilic compounds may more easily pass through the cell membrane of V. fischeri than the gill and skin of fish, react with the target macromolecules and exhibit excess toxicity. The compounds with log KOW > 7 exhibiting very low toxicity (log TR < -1) to both species indicate that the bioconcentration potential of a chemical plays a very important role in the identification of excess toxicity and MOAs

  16. Comparison of Toxicities to Vibrio fischeri and Fish Based on Discrimination of Excess Toxicity from Baseline Level.

    Directory of Open Access Journals (Sweden)

    Xiao H Wang

    Full Text Available Investigations on the relationship of toxicities between species play an important role in the understanding of toxic mechanisms to environmental organisms. In this paper, the toxicity data of 949 chemicals to fish and 1470 chemicals to V. fischeri were used to investigate the modes of action (MOAs between species. The results show that although there is a positive interspecies correlation, the relationship is poor. Analysis on the excess toxicity calculated from toxic ratios (TR shows that many chemicals have close toxicities and share the same MOAs between the two species. Linear relationships between the toxicities and octanol/water partition coefficient (log KOW for baseline and less inert compounds indicate that the internal critical concentrations (CBRs approach a constant both to fish and V. fischeri for neutral hydrophobic compounds. These compounds share the same toxic mechanisms and bio-uptake processes between species. On the other hand, some hydrophilic compounds exhibit different toxic effects with greatly different log TR values between V. fischeri and fish species. These hydrophilic compounds were identified as reactive MOAs to V. fischeri, but not to fish. The interspecies correlation is improved by adding a hydrophobic descriptor into the correlation equation. This indicates that the differences in the toxic ratios between fish and V. fischeri for these hydrophilic compounds can be partly attributed to the differences of bioconcentration between the two species, rather than the differences of reactivity with the target macromolecules. These hydrophilic compounds may more easily pass through the cell membrane of V. fischeri than the gill and skin of fish, react with the target macromolecules and exhibit excess toxicity. The compounds with log KOW > 7 exhibiting very low toxicity (log TR < -1 to both species indicate that the bioconcentration potential of a chemical plays a very important role in the identification of excess

  17. 杭州地区部分人群糖化血红蛋白的调查研究%Study on the level of glycosylated hemoglobin in physical examination population in Hangzhou city

    Institute of Scientific and Technical Information of China (English)

    马小红; 庄爱周; 李萌; 万晓晨

    2012-01-01

    目的:研究杭州地区部分人群糖化血红蛋白(glycosylated hemoglobin,Hb)水平的分布情况.方法:收集2010年8月- 2011年8月在我院体检的4850例体检人员样本,应用全自动糖化血红蛋白分析仪检测HbA1(%)和HbA1c(%)水平,分别按性别、年龄、HbA1c参考值及2010年糖尿病诊断新标准对样本进行分组,并进行统计分析.结果:结果表明,HbA1(%)和HbA1c(%)在不同性别和年龄的人群中的分布有显著性差异.结论:杭州地区男性Hb总体水平均明显高于女性,且随着年龄的增长Hb水平增高,且根据2010年新指南筛查出疑似糖尿病者增多,为糖尿病的早期发现、早期治疗及减少并发症的发生提供了前提.%Objective:To investigate the distribution of glycosylated hemoglobin (Hb) level in Hangzhou population. Methods:4850 cases of physical examination individuals in our hospital from 2010 August to 2011 August were recruited into this stud-y. HbAl (% ) and HbAlc (% ) level were detected by the automatic glycosylated hemoglobin analyzer, and the results were conducted statistical analysis by sex, age, the reference value of HbAlc and the 2010 new criteria for diagnosis of diabetes melli-tus, separately. Results:The results show that the distribution of HbAl (% ) and HbAlc (% ) levels existed significant difference in different genders and age groups of Hangzhou population. Conclusion:The Hb level of male in Hangzhou were significantly higher than that of female, and the Hb level became higher as the age increased. According to new 2010 guidelines, the suspected diabetes increased, which has provided basis for early detection, early treatment of diabetes and reduce the incidence of diabetes complications.

  18. Annotated definition of BCL11A and HMIP-2 haplotypes through the analysis of sicilian β-thalassemia patients with high levels of fetal hemoglobin.

    Science.gov (United States)

    Buccheri, Maria A; Spina, Sonia; Ruberto, Concetta; Lombardo, Turi; Labie, Dominique; Ragusa, And Angela

    2013-01-01

    Fetal hemoglobin (Hb F) is the principal ameliorating factor of β-thalassemia (β-thal) and sickle cell disease. Persistent production in adult life is a quantitative trait regulated by loci inside or outside the β-globin gene cluster. From genome-wide association studies, principal quantitative trait loci (QTL) (accounting for 50.0% of Hb F variability in different populations) have been identified in the BCL11A gene, HBS1L-MYB intergenic polymorphism and the β-globin gene cluster itself. In this study, we analyzed quantitative trait haplotypes in two Sicilian families with extremely mild β-thal and unusually high Hb F expression, in order to examine possible genetic background variations in a similar β-thalassemic phenotype. This study redefines the linkage disequilibrium blocks at these loci, but also shows slight differences between probands in haplotype combinations which could reflect different mechanisms of high Hb F production in patients with β-thal. We proposed a haplotype-based approach as a useful tool for the understanding of β-thal phenotype variation in patients with similar β-thalassemic backgrounds in an attempt to answer the recurring question of why patients with the same β-thalassemic genotype show different phenotypes. PMID:23777413

  19. Niveles de hemoglobina en gestantes atendidas en establecimientos del Ministerio de Salud del Perú, 2011 Hemoglobin levels in pregnant women seen in health care centers of the peruvian ministry of health, 2011

    Directory of Open Access Journals (Sweden)

    Oscar Munares-García

    2012-09-01

    Full Text Available Objetivos. Determinar los niveles de hemoglobina y la prevalencia de anemia en gestantes atendidas en los establecimientos del Ministerio de Salud a nivel nacional. Materiales y métodos. Estudio transversal donde se analizó la base de datos del Sistema de Información del Estado Nutricional del Niño menor de 5 años y de la Gestante (SIEN. Se incluyó 287 691 registros de gestantes evaluadas en establecimientos del Ministerio de Salud del Perú en 2011, se analizaron los niveles de hemoglobina corregida a la altura, edad, edad gestacional, altitud a nivel del mar y prevalencia de anemia (leve, moderada y grave. Se aplicaron estadísticas descriptivas y chi cuadrado. Resultados. La prevalencia a nivel nacional de anemia en la gestante fue de 28,0% siendo anemia leve de 25,1%, moderada de 2,6% y grave de 0,2%. Los niveles de hemoglobina son mayores en mujeres con mayor edad y menores durante los primeros meses de gestación, la frecuencia de anemia decrece con la altitud. Asimismo, la prevalencia es mayor en departamentos de la sierra. Huancavelica fue el departamento con mayor prevalencia de anemia (53,6%, seguido de Puno con 51,0%. Conclusiones. Los niveles de hemoglobina son mayores conforme la edad materna es mayor, y menores conforme el trimestre de gestación y altitud. Huancavelica tiene la mayor prevalencia de anemia en gestantesObjectives. Determine hemoglobin levels and prevalence of anemia in pregnant women seen in health care centers of the Ministry of Health at national level. Materials and methods. Cross-cut study where the database of the Information System on the Nutritional Health of Children under 5 and Pregnant Women (SIEN were analyzed. 287 691 records of pregnant women examined at the health care centers of the Peruvian Ministry of Health in 2011 were included, hemoglobin levels corrected by height, age, gestational age, altitude and prevalence of anemia (light, moderate and serious were analyzed. Descriptive statistics and

  20. Genetic polymorphism rs6922269 in the MTHFD1L gene is associated with survival and baseline active vitamin B12 levels in post-acute coronary syndromes patients.

    Directory of Open Access Journals (Sweden)

    Barry R Palmer

    Full Text Available BACKGROUND AND AIMS: The methylene-tetrahydrofolate dehydrogenase (NADP+ dependent 1-like (MTHFD1L gene is involved in mitochondrial tetrahydrofolate metabolism. Polymorphisms in MTHFD1L, including rs6922269, have been implicated in risk for coronary artery disease (CAD. We investigated the association between rs6922269 and known metabolic risk factors and survival in two independent cohorts of coronary heart disease patients. METHODS AND RESULTS: DNA and plasma from 1940 patients with acute coronary syndromes were collected a median of 32 days after index hospital admission (Coronary Disease Cohort Study, CDCS. Samples from a validation cohort of 842 patients post-myocardial infarction (PMI were taken 24-96 hours after hospitalization. DNA samples were genotyped for rs6922269, using a TaqMan assay. Homocysteine and active vitamin B12 were measured by immunoassay in baseline CDCS plasma samples, but not PMI plasma. All cause mortality was documented over follow-up of 4.1 (CDCS and 8.8 (PMI years, respectively. rs6922269 genotype frequencies were AA n = 135, 7.0%; GA n = 785, 40.5% and GG n = 1020, 52.5% in the CDCS and similar in the PMI cohort. CDCS patients with AA genotype for rs6922269 had lower levels of co-variate adjusted baseline plasma active vitamin B12 (p = 0.017 and poorer survival than patients with GG or GA genotype (mortality: AA 19.6%, GA 12.0%, GG 11.6%; p = 0.007. In multivariate analysis, rs6922269 genotype predicted survival, independent of established covariate predictors (p = 0.03. However the association between genotype and survival was not validated in the PMI cohort. CONCLUSION: MTHFD1L rs6922269 genotype is associated with active vitamin B12 levels at baseline and may be a marker of prognostic risk in patients with established coronary heart disease.

  1. Right hemisphere dominance directly predicts both baseline V1 cortical excitability and the degree of top-down modulation exerted over low-level brain structures.

    Science.gov (United States)

    Arshad, Q; Siddiqui, S; Ramachandran, S; Goga, U; Bonsu, A; Patel, M; Roberts, R E; Nigmatullina, Y; Malhotra, P; Bronstein, A M

    2015-12-17

    Right hemisphere dominance for visuo-spatial attention is characteristically observed in most right-handed individuals. This dominance has been attributed to both an anatomically larger right fronto-parietal network and the existence of asymmetric parietal interhemispheric connections. Previously it has been demonstrated that interhemispheric conflict, which induces left hemisphere inhibition, results in the modulation of both (i) the excitability of the early visual cortex (V1) and (ii) the brainstem-mediated vestibular-ocular reflex (VOR) via top-down control mechanisms. However to date, it remains unknown whether the degree of an individual's right hemisphere dominance for visuospatial function can influence, (i) the baseline excitability of the visual cortex and (ii) the extent to which the right hemisphere can exert top-down modulation. We directly tested this by correlating line bisection error (or pseudoneglect), taken as a measure of right hemisphere dominance, with both (i) visual cortical excitability measured using phosphene perception elicited via single-pulse occipital trans-cranial magnetic stimulation (TMS) and (ii) the degree of trans-cranial direct current stimulation (tDCS)-mediated VOR suppression, following left hemisphere inhibition. We found that those individuals with greater right hemisphere dominance had a less excitable early visual cortex at baseline and demonstrated a greater degree of vestibular nystagmus suppression following left hemisphere cathodal tDCS. To conclude, our results provide the first demonstration that individual differences in right hemisphere dominance can directly predict both the baseline excitability of low-level brain structures and the degree of top-down modulation exerted over them. PMID:26518461

  2. Potential Effects of a Scenario Earthquake on the Economy of Southern California: Baseline County-Level Migration Characteristics and Trends 1995-2000 and 2001-2010

    Science.gov (United States)

    Sherrouse, Benson C.; Hester, David J.

    2008-01-01

    The Multi-Hazards Demonstration Project (MHDP) is a collaboration between the U.S. Geological Survey (USGS) and various partners from the public and private sectors and academia, meant to improve Southern California's resiliency to natural hazards. In support of the MHDP objectives, the ShakeOut Scenario was developed. It describes a magnitude 7.8 earthquake along the southernmost 300 kilometers (200 miles) of the San Andreas Fault, identified by geoscientists as a plausible event that will cause moderate to strong shaking over much of the eight-county (Imperial, Kern, Los Angeles, Orange, Riverside, San Bernardino, San Diego, and Ventura) Southern California region. This report uses historical, estimated, and projected population data from several Federal and State data sources to estimate baseline characteristics and trends of the region's population migration (that is, changes in a person's place of residence over time). The analysis characterizes migration by various demographic, economic, family, and household variables for the period 1995-2000. It also uses existing estimates (beginning in 2001) of the three components of population change - births, deaths, and migration - to extrapolate near-term projections of county-level migration trends through 2010. The 2010 date was chosen to provide baseline projections corresponding to a two-year recovery period following the November 2008 date that was selected for the occurrence of the ShakeOut Scenario earthquake. The baseline characteristics and projections shall assist with evaluating the effects of inflow and outflow migration trends for alternative futures in which the simulated M7.8 earthquake either does or does not occur and the impact of the event on housing and jobs, as well as community composition and regional economy changes based on dispersion of intellectual, physical, economic, and cultural capital.

  3. Right hemisphere dominance directly predicts both baseline V1 cortical excitability and the degree of top-down modulation exerted over low-level brain structures.

    Science.gov (United States)

    Arshad, Q; Siddiqui, S; Ramachandran, S; Goga, U; Bonsu, A; Patel, M; Roberts, R E; Nigmatullina, Y; Malhotra, P; Bronstein, A M

    2015-12-17

    Right hemisphere dominance for visuo-spatial attention is characteristically observed in most right-handed individuals. This dominance has been attributed to both an anatomically larger right fronto-parietal network and the existence of asymmetric parietal interhemispheric connections. Previously it has been demonstrated that interhemispheric conflict, which induces left hemisphere inhibition, results in the modulation of both (i) the excitability of the early visual cortex (V1) and (ii) the brainstem-mediated vestibular-ocular reflex (VOR) via top-down control mechanisms. However to date, it remains unknown whether the degree of an individual's right hemisphere dominance for visuospatial function can influence, (i) the baseline excitability of the visual cortex and (ii) the extent to which the right hemisphere can exert top-down modulation. We directly tested this by correlating line bisection error (or pseudoneglect), taken as a measure of right hemisphere dominance, with both (i) visual cortical excitability measured using phosphene perception elicited via single-pulse occipital trans-cranial magnetic stimulation (TMS) and (ii) the degree of trans-cranial direct current stimulation (tDCS)-mediated VOR suppression, following left hemisphere inhibition. We found that those individuals with greater right hemisphere dominance had a less excitable early visual cortex at baseline and demonstrated a greater degree of vestibular nystagmus suppression following left hemisphere cathodal tDCS. To conclude, our results provide the first demonstration that individual differences in right hemisphere dominance can directly predict both the baseline excitability of low-level brain structures and the degree of top-down modulation exerted over them.

  4. Hemoglobin variants in Cyprus.

    Science.gov (United States)

    Kyrri, Andreani R; Felekis, Xenia; Kalogerou, Eleni; Wild, Barbara J; Kythreotis, Loukas; Phylactides, Marios; Kleanthous, Marina

    2009-01-01

    Cyprus, located at the eastern end of the Mediterranean region, has been a place of eastern and western civilizations, and the presence of various hemoglobin (Hb) variants can be considered a testimony to past colonizations of the island. In this study, we report the structural Hb variants identified in the Cypriot population (Greek Cypriots, Maronites, Armenians, and Latinos) during the thalassemia screening of 248,000 subjects carried out at the Thalassaemia Centre, Nicosia, Cyprus, over a period of 26 years. A sample population of 65,668 people was used to determine the frequency and localization of several of the variants identified in Cyprus. The localization of some of the variants in regions where the presence of foreign people was most prevalent provides important clues to the origin of the variants. Twelve structural variants have been identified by DNA sequencing, nine concerning the beta-globin gene and three concerning the alpha-globin gene. The most common beta-globin variants identified were Hb S (0.2%), Hb D-Punjab (0.02%), and Hb Lepore-Washington-Boston (Hb Lepore-WB) (0.03%); the most common alpha-globin variant was Hb Setif (0.1%). The presence of some of these variants is likely to be directly linked to the history of Cyprus, as archeological monuments have been found throughout the island which signify the presence for many years of the Greeks, Syrians, Persians, Arabs, Byzantines, Franks, Venetians, and Turks. PMID:19373583

  5. Expression of fully functional tetrameric human hemoglobin in Escherichia coli

    Energy Technology Data Exchange (ETDEWEB)

    Hoffman, S.J.; Looker, D.L.; Roehrich, J.M.; Cozart, P.E.; Durfee, S.L.; Tedesco, J.L.; Stetler, G.L. (Somatogen, Inc., Broomfield, CO (United States))

    1990-11-01

    Synthesis genes encoding the human {alpha}- and {beta}-globin polypeptides have been expressed from a single operon in Escherichia coli. The {alpha}- and {beta}-globin polypeptides associate into soluble tetramers, incorporate heme, and accumulate to >5% of the total cellular protein. Purified recombinant hemoglobin has the correct stoichiometry of {alpha}- and {beta}-globin chains and contains a full complement of heme. Each globin chain also contains an additional methionine as an extension to the amino terminus. The recombinant hemoglobin has a C{sub 4} reversed-phase HPLC profile essentially identical to that of human hemoglobin A{sub 0} and comigrates with hemoglobin A{sub 0} on SDS/PAGE. The visible spectrum and oxygen affinity are similar to that of native human hemoglobin A{sub 0}. The authors have also expressed the {alpha}- and {beta}-globin genes separately and found that the expression of the {alpha}-globin gene alone results in a marked decrease in the accumulation of {alpha}-globin in the cell. Separate expression of the {beta}-globin gene results in high levels of insoluble {beta}-globin. These observations suggest that the presence of {alpha}- and {beta}-globin in the same cell stabilizes {alpha}-globin and aids the correct folding of {beta}-globin. This system provides a simple method for expressing large quantities of recombinant hemoglobin and allows facile manipulation of the genes encoding hemoglobin to produce functionally altered forms of this protein.

  6. Fetal hemoglobin in sickle cell anemia.

    Science.gov (United States)

    Akinsheye, Idowu; Alsultan, Abdulrahman; Solovieff, Nadia; Ngo, Duyen; Baldwin, Clinton T; Sebastiani, Paola; Chui, David H K; Steinberg, Martin H

    2011-07-01

    Fetal hemoglobin (HbF) is the major genetic modulator of the hematologic and clinical features of sickle cell disease, an effect mediated by its exclusion from the sickle hemoglobin polymer. Fetal hemoglobin genes are genetically regulated, and the level of HbF and its distribution among sickle erythrocytes is highly variable. Some patients with sickle cell disease have exceptionally high levels of HbF that are associated with the Senegal and Saudi-Indian haplotype of the HBB-like gene cluster; some patients with different haplotypes can have similarly high HbF. In these patients, high HbF is associated with generally milder but not asymptomatic disease. Studying these persons might provide additional insights into HbF gene regulation. HbF appears to benefit some complications of disease more than others. This might be related to the premature destruction of erythrocytes that do not contain HbF, even though the total HbF concentration is high. Recent insights into HbF regulation have spurred new efforts to induce high HbF levels in sickle cell disease beyond those achievable with the current limited repertory of HbF inducers. PMID:21490337

  7. Lower versus Higher Hemoglobin Threshold for Transfusion in Septic Shock

    DEFF Research Database (Denmark)

    Holst, Lars B; Haase, Nicolai; Wetterslev, Jørn;

    2014-01-01

    BACKGROUND: Blood transfusions are frequently given to patients with septic shock. However, the benefits and harms of different hemoglobin thresholds for transfusion have not been established. METHODS: In this multicenter, parallel-group trial, we randomly assigned patients in the intensive care...... unit (ICU) who had septic shock and a hemoglobin concentration of 9 g per deciliter or less to receive 1 unit of leukoreduced red cells when the hemoglobin level was 7 g per deciliter or less (lower threshold) or when the level was 9 g per deciliter or less (higher threshold) during the ICU stay...... were similar in the two intervention groups. CONCLUSIONS: Among patients with septic shock, mortality at 90 days and rates of ischemic events and use of life support were similar among those assigned to blood transfusion at a higher hemoglobin threshold and those assigned to blood transfusion...

  8. Baseline-Corrected Second-Order Derivative Electroanalysis Combined With Ultrasound-Assisted Liquid-Liquid Microextraction: Simultaneous Quantification of Fluoroquinolones at Low Levels.

    Science.gov (United States)

    de Oliveira, Luiz Henrique; Trindade, Magno Aparecido Gonçalves

    2016-06-21

    A baseline-corrected second-order derivative procedure and a miniaturized sample preparation based on low-density solvent and ultrasound-assisted liquid-liquid microextraction (LDS-UA-LLME) was combined to provide the simultaneous electroanalysis of three fluoroquinolones (FQ) as emerging contaminants (ECs). The enhanced mathematical processing provided the best separation with an accurate measurement of the overlapping peaks during the simultaneous electro-oxidation of target FQs that were directly dropped on the surface of carbon nanofiber-modified screen-printed electrodes. The adapted LDS-UA-LLME protocol was the key step involved in the sample preparation, which preconcentrate target analytes from diluted tap water samples with an enrichment factor of around 80×, allowing their quantification at trace levels. This combined feature demonstrated the unique application of an electroanalytical technique for the simultaneous electroanalysis of three FQs in spiked tap water samples, with recovery values remarkably close to 100%. PMID:27249314

  9. Baseline-Corrected Second-Order Derivative Electroanalysis Combined With Ultrasound-Assisted Liquid-Liquid Microextraction: Simultaneous Quantification of Fluoroquinolones at Low Levels.

    Science.gov (United States)

    de Oliveira, Luiz Henrique; Trindade, Magno Aparecido Gonçalves

    2016-06-21

    A baseline-corrected second-order derivative procedure and a miniaturized sample preparation based on low-density solvent and ultrasound-assisted liquid-liquid microextraction (LDS-UA-LLME) was combined to provide the simultaneous electroanalysis of three fluoroquinolones (FQ) as emerging contaminants (ECs). The enhanced mathematical processing provided the best separation with an accurate measurement of the overlapping peaks during the simultaneous electro-oxidation of target FQs that were directly dropped on the surface of carbon nanofiber-modified screen-printed electrodes. The adapted LDS-UA-LLME protocol was the key step involved in the sample preparation, which preconcentrate target analytes from diluted tap water samples with an enrichment factor of around 80×, allowing their quantification at trace levels. This combined feature demonstrated the unique application of an electroanalytical technique for the simultaneous electroanalysis of three FQs in spiked tap water samples, with recovery values remarkably close to 100%.

  10. Steady state hemoglobin concentration and packed cell volume in homozygous sickle cell disease patients in Lagos, Nigeria

    OpenAIRE

    Akinbami, Akinsegun; Dosunmu, Adedoyin; Adediran, Adewumi; Oshinaike, Olajumoke; Phillip, Adebola; Vincent, Osunkalu; Olanrewaju, Arogundade; Oluwaseun, Adelekan

    2012-01-01

    Background: Sickle cell disease is a genetic disorder of hemoglobin causing myriad of pathology including anemia. The purpose of this study was to evaluate the baseline values of steady state hemoglobin and packed cell volume as a guide to managing the early recognition of hemolytic crises in sickle cell anemia.

  11. Nonlinear photoacoustic spectroscopy of hemoglobin

    Energy Technology Data Exchange (ETDEWEB)

    Danielli, Amos; Maslov, Konstantin; Favazza, Christopher P.; Xia, Jun; Wang, Lihong V., E-mail: LHWANG@WUSTL.EDU [Optical Imaging Laboratory, Department of Biomedical Engineering, Washington University in St. Louis, One Brookings Drive, St. Louis, Missouri 63130 (United States)

    2015-05-18

    As light intensity increases in photoacoustic imaging, the saturation of optical absorption and the temperature dependence of the thermal expansion coefficient result in a measurable nonlinear dependence of the photoacoustic (PA) signal on the excitation pulse fluence. Here, under controlled conditions, we investigate the intensity-dependent photoacoustic signals from oxygenated and deoxygenated hemoglobin at varied optical wavelengths and molecular concentrations. The wavelength and concentration dependencies of the nonlinear PA spectrum are found to be significantly greater in oxygenated hemoglobin than in deoxygenated hemoglobin. These effects are further influenced by the hemoglobin concentration. These nonlinear phenomena provide insights into applications of photoacoustics, such as measurements of average inter-molecular distances on a nm scale or with a tuned selection of wavelengths, a more accurate quantitative PA tomography.

  12. Localised IR spectroscopy of hemoglobin

    CERN Document Server

    Yarrow, Fiona

    2010-01-01

    IR absorption spectroscopy of hemoglobin was performed using an IR optical parametric oscillator laser and a commercial atomic force microscope in a novel experimental arrangement based on the use of a bottom-up excitation alignment. This experimental approach enables detection of protein samples with a resolution that is much higher than that of standard IR spectroscopy. Presented here are AFM based IR absorption spectra of micron sized hemoglobin features

  13. Hemoglobin concentrations and associated factors in adolescentes from Recife, Brazil

    OpenAIRE

    Elisângela Barros Soares Mendonça; Lilian Ferreira Muniz; Ilma Kruze Grande de Arruda; Alcides da Silva Diniz

    2014-01-01

    OBJECTIVE: To estimate the prevalence of anemia and associated factors in adolescents from the city of Recife in Pernambuco state. METHODS: This is a cross-sectional study, involving a random sample of 256 adolescents of both genders, aged 13 to 18, whose hemoglobin concentrations were evaluated, along with their nutritional status and socioeconomic and demographic characteristics. RESULTS: The prevalence of inadequate hemoglobin concentrations was 10.2% [CI95%=6.7-14.5], reaching levels cons...

  14. Comparison between lead levels in dandelions grown in an ultra-clean lab environment (baseline) and those collected from the San Francisco Bay Area

    Science.gov (United States)

    Rojero, J.; Odigie, K. O.; Hibdon, S.; Flegal, A. R.

    2011-12-01

    This study is aimed at establishing the baseline (natural) levels of lead in dandelions (Taraxacum officinale) grown in an ultra-clean environment. Dandelions have been used extensively as biomonitors of environmental lead levels since their distribution is global and they can be easily collected. However, industrial lead contamination is so pervasive that even dandelions from the most remote areas in the world may be contaminated with industrial lead. Therefore, this work will test the hypothesis that "natural" lead levels in dandelions are lower than any previously published values - by growing them in a HEPA filtered air (Class 100) trace metal clean room with high purity (18 MΩ cm) water. Concentrations and isotopic compositions of lead in the clean-room grown dandelions will be compared to values in literature and to those of lead in dandelions collected from San Francisco Bay Area. Lead is a dense, ductile, and highly malleable metal that is found naturally in our environment. Due to its properties it is currently highly used in building construction, in ceramic glazes, lead chromate and in PVC plastic used to coat electrical cords. The uses of lead have included paint, leather tanning, and being used as an additive to gasoline prior to the mid 1970's, as well as others. Due to its many uses, humans are susceptible to lead regularly through various means of exposure from air, water and soil, often leading to lead toxicity.

  15. To evaluate the levels of glycated hemoglobin, serum calcium, magnesium, phosphate, uric acid and microalbuminuria in patients with newly diagnosed type 2 diabetes mellitus

    Directory of Open Access Journals (Sweden)

    Qazi Najeeb

    2014-08-01

    Conclusion: There is decrease in serum calcium, magnesium and phosphate levels, all these plays an important role in the regulation of glucose level in the blood. Hence oral supplementation of all these ions other than diet is recommended. Increased serum uric acid and microalbuminuria was seen with reduced glucose tolerance hence early estimation of both the parameters should be done while monitoring case of Type-2 diabetes and thus will help to decrease the incidence of renal complications. [Int J Res Med Sci 2014; 2(4.000: 1462-1465

  16. Study on the Clinical Relationship between Blood Glucose, Glycated Hemoglobin Level and Acute Cerebral Infarction%血糖、糖化血红蛋白水平与急性脑梗死临床关系的探讨

    Institute of Scientific and Technical Information of China (English)

    袁勇

    2012-01-01

      Objective To investigate acute patient of brain infarct blood sugar competence and clinical relation. Methods Empty stomach blood sugar level when admitting to hospital divide into normal blood sugar group,high blood sugar group, at random according to patient, Before treating, after treating,flawed or damaged to go on and give a mark to nervous system,Observe blood sugar, glycosylated hemoglobin have illness coming on the total mark and clinical relation. Results Acute brain infarct blood sugar level high,and have illness coming on and flawed or damaged to give a mark either high nervous system of patient,the worse its clinical curative effect is. Conclusion High bloods sugars accompany high glycosylated hemoglobin increase acute brain nervous system of infarct damage especially with have illness coming on. Lower candy treatment may improve acute brain clinical curative effect of infarct effectively,Prevent and treat high blood sugar to prevent brain infarct effective measure one of actively.%  目的探讨血糖、糖化血红蛋白水平与急性脑梗死患者的临床关系。方法对收治的78例急性脑梗死患者的临床资料进行回顾性分析,根据入院时空腹血糖水平分为正常血糖组、高血糖组,病情好转后对所有患者进行神经系统缺损评分,观察血糖、糖化血红蛋白和临床的关系。结果急性脑梗死患者高血糖组与正常血糖组相比,其神经系统缺损评分高,总有效率低。结论高血糖尤其伴高糖化血红蛋白会增加急性脑梗死神经系统损伤,预后差。故积极预防和治疗高血糖是预防脑梗死的有力措施之一。

  17. The microsatellite polymorphism of heme oxygenase-1 is associated with baseline plasma IL-6 level but not with restenosis after coronary in-stenting

    Institute of Scientific and Technical Information of China (English)

    LI Ping; Mohamed A. Elrayess; Abuzeid H. Gomma; Jutta Palmen; Emma Hawe; Kim M. Fox; Steve E.Humphries

    2005-01-01

    Background Vascular smooth muscle cells (VSMCs) can express heme-oxygenase (HO), a rate-limiting enzyme in the degradation of heme to bilirubin, ferritin and carbon monoxide (CO). VSMC-derived CO can suppress VSMC proliferation and 05-serve as an antiproliferation factor. The promoter region of HO-1 shows a polymorphism with different (GT)n repeats that has been reported to differently induce gene expression. The objective of this study was to examine the effect of this variation on the occurrence of restenosis after in-stent treatment in patients with coronary artery disease. Methods Candidates who underwent coronary stent implantation were genotyped for the HO-1 promoter polymorphism using polymerase chain reaction (PCR) and automated DNA capillary sequencer. Serum levels of IL-6 and C-reactive protein (CRP) were obtained at baseline, 24 hours and 48 hours after stenting. The primary end point for the study was angiographic evidence of in-stent restenosis at 6 months. All parameters for evaluation of restenosis were analysed by quantitatve computer-assisted angiographic analysis (QCA). Results One hundred and eighty-seven patients who underwent coronary stent implantation were studied of whom 27.8% showed ≥50% restenosis after 6 months. The distribution of (GT)n repeats of all patients in the promoter region of HO-1 genotype ranged from 22 to 42, with (GT)25 and (GT)32 being the two most common alleles. The allelic repeats were divided into the short class (S) with 29 (GT)n, the middle class (M) with 30-37 (GT)n and the long class (L) with 38 (GT)n. There was no significant difference in the restenosis between the genotype groups or between post operation levels of inflammation markers, but carriers of the S allele (n=120) had 33.3% lower baseline IL-6 compared with non-S carriers (n=67, P=0.0008). Conclusions Although no association was observed between the HO-1 promoter polymorphism and coronary in-stent restenosis following the stent procedure, the

  18. Measurement of Persistent Organic Pollutants (POPs) in plastic resin pellets from remote islands : Toward establishment of baseline level for International Pellet Watch

    Science.gov (United States)

    Takada, H.; Heskett, M.; Yamashita, R.; Yuyama, M.; Itoh, M.; Geok, Y. B.; Ogata, Y.

    2011-12-01

    Plastic resin pellets collected from remote islands in open oceans (Canary, St. Helena, Cocos, Hawaii, Maui Islands and Barbados) were sorted and yellowing polyethylene (PE) pellets were measured for polychlorinated biphenyls (PCBs), dichlorodiphenyltrichloroethane and the degradation products (DDTs), and hexachlorocyclohexanes (HCHs) by gas chromatograph equipped with mass spectrometer (GC-MS) and with electron capture detector (GC-ECD). PCBs were detected from all the pellet samples, confirming the global dispersion of PCBs. Median concentrations of PCBs (sum of 13 congeners : CB-66, CB-101, CB-110, CB-118, CB-105, CB-149, CB-153, CB-138, CB-128, CB-187, CB-180, CB-170, CB-206) in the remote island pellets ranged from 0.1 to 10 ng/g-pellet. These were one to three orders of magnitude lower than those observed for pellets from industrialized coastal zones (hundreds ng/g in Los Angeles, Boston, Tokyo; Ogata et al., 2009). Because these remote islands are far (>100 km) from industrialized zones, these concentrations (i.e., 0.1 to 10 ng/g-pellet) can be regarded as global "baseline" level of PCB pollution. Concentrations of DDTs in the remote island pellets ranged from 0.2 to 5.5 ng/g-pellet. At some locations, DDT was dominant over the degradation products (DDE and DDD), suggesting current usage of the pesticides in the islands. HCHs concentrations were 0.4 - 1.8 ng/g-pellet and lower than PCBs and DDTs, except for St. Helena Island at 18.8 ng/g-pellet where the current usage of the pesticides are of concern. The analyses of pellets from the remote islands provided "baseline" level of POPs (PCBs plastic debris which were contaminated in industrialized coastal zones may have rapidly transported to the remote islands before they would reach equilibrium (i.e., desorption completed). Because POPs concentrations in the other media are at trace levels in these remote environments, the sporadic high concentrations of POPs in the plastic debris may pose threat to the

  19. Proposal to generate 10 TW level femtosecond X-ray pulses from a baseline undulator in conventional SASE regime at the European XFEL

    Energy Technology Data Exchange (ETDEWEB)

    Serkez, Svitozar; Kocharyan, Vitali; Saldin, Evgeni; Zagorodnov, Igor [Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany); Geloni, Gianluca [European XFEL GmbH, Hamburg (Germany)

    2013-08-15

    Output characteristics of the European XFEL have been previously studied assuming an operation point at 5 kA peak current. In this paper we explore the possibility to go well beyond such nominal peak current level. In order to illustrate the potential of the European XFEL accelerator complex we consider a bunch with 0.25 nC charge, compressed up to a peak current of 45 kA. An advantage of operating at such high peak current is the increase of the X-ray output peak power without any modification to the baseline design. Based on start-to-end simulations, we demonstrate that such high peak current, combined with undulator tapering, allows one to achieve up to a 100-fold increase in a peak power in the conventional SASE regime, compared to the nominal mode of operation. In particular, we find that 10 TW-power level, femtosecond x-ray pulses can be generated in the photon energy range between 3 keV and 5 keV, which is optimal for single biomolecule imaging. Our simulations are based on the exploitation of all the 21 cells foreseen for the SASE3 undulator beamline, and indicate that one can achieve diffraction to the desired resolution with 15 mJ (corresponding to about 3.10{sup 13} photons) in pulses of about 3 fs, in the case of a 100 nm focus at the photon energy of 3.5 keV.

  20. A baseline study of levels of mercury, arsenic, cadmium and lead in Northeast Arctic cod (Gadus morhua) from different parts of the Barents Sea

    International Nuclear Information System (INIS)

    Highlights: ► Highest concentration of Hg was found in the western part of the Barents Sea. ► The Hg concentrations were well below the maximum level of 0.5 mg kg−1 ► Arsenic concentrations varied greatly ranging from 0.3 to 170 mg kg−1 ww. ► Shrimp seems to be the most important prey for cod with high concentration of As. - Abstract: This study is one of several baseline studies that will provide basic and reliable information about the content of undesirable substances in important species of fish caught in Norwegian waters. Concentrations of metals in the muscle and liver of more than 800 Northeast Arctic cod caught at 32 sites in the Barents Sea are reported. The highest concentration of both mercury in the muscle and cadmium in the liver was found in cod caught in the western part of the Barents Sea, while the highest concentration of total arsenic was found in cod from the eastern part. The arsenic concentrations varied greatly among individual fish, ranging from 0.3 to 170 mg kg−1 wet weight in the muscle. Such high levels of total arsenic have never previously been reported in any fish, and the primary factor for these high concentrations is likely to be the shrimp in the cod diet

  1. Survey of Air Pollution Effect on Variation of Glycosylated Hemoglobin A1C (HbA1C level in Diabetic Patients in Tehran

    Directory of Open Access Journals (Sweden)

    Fatemeh Mousavi

    2013-05-01

    Materials and Methods: During November-January 2010-11, Tehran, capital of Iran, was exposed with high levels of air pollution. A retrospective cohort study was carried out on 330 patients diagnosed with diabetes mellitus for at least 12 months referring to 3 endocrinal care clinics. A questionnaire in two demographic and diabetic related sections was prepared. The patients' HbA1C level recorded on November-January 2009-10 was compared with November-January 2010-11. Descriptive analysis and paired t-test were carried out using SPSS 18 software. Results: The patients investigated were divided into two groups. The first group was composed of 108 patients (53.7% female and 46.3% male with diabetes mellitus type I (Insulin Dependent, age mean of 17.22, and SD of 11.57. The second group was composed of 222 patients (58.6% female and 41.4% male with diabetes mellitus type II (Noninsulin Dependent, age mean of 53.91, and SD of 12.12. The change of HbAIC level in both groups wa not statistically significant; in first group, HbA1C level increased from 7.71 to 7.75 mg / 100 ml (P =0.828 and in second group, it increased from 7.06 to 7.08 mg / 100 ml (P = 0.798. Conclusion: According to the results obtained, it can be concluded that relation of air pollution and HbA1C mean variation in diabetic patients was insignificant.

  2. Effect of low-level laser therapy on blood flow and oxygen- hemoglobin saturation of the foot skin in healthy subjects: a pilot study

    OpenAIRE

    Heu, Franziska; Forster, Clemens; Namer, Barbara; Dragu, Adrian; Lang, Werner

    2013-01-01

    Background and aims: This study on healthy test subjects intends to show whether one-off Low-Level Laser Therapy (LLLT) has an instant effect on the perfusion or the oxygenation of the skin tissue. These possible instant effects may have an influence on the accelerated wound healing which is often observed after application of LLLT, in addition to the usual postulated effects of LLLT which occur with a time delay normally.

  3. Hemoglobin Screening Independently Predicts All-Cause Mortality.

    Science.gov (United States)

    Fulks, Michael; Dolan, Vera F; Stout, Robert L

    2015-01-01

    Objective .- Determine if the addition of hemoglobin testing improves risk prediction for life insurance applicants. Method .- Hemoglobin results for insurance applicants tested from 1993 to 2007, with vital status determined by Social Security Death Master File follow-up in 2011, were analyzed by age and sex with and without accounting for the contribution of other test results. Results .- Hemoglobin values ≤12.0 g/dL (and possibly ≤13.0 g/dL) in females age 50+ (but not age 15.0 g/dL (and possibly >14.0 g/dL) for all females and for hemoglobin values >16.0 g/dL for males. Conclusion .- Hemoglobin testing can add additional independent risk assessment to that obtained from other laboratory testing, BP and build in this relatively healthy insurance applicant population. Multiple studies support this finding at older ages, but data (and the prevalence of diseases impacting hemoglobin levels) are limited at younger ages.

  4. Hemoglobin and heme scavenger receptors

    DEFF Research Database (Denmark)

    Nielsen, Marianne Jensby; Møller, Holger Jon; Moestrup, Søren Kragh

    2010-01-01

    Heme, the functional group of hemoglobin, myoglobin, and other hemoproteins, is a highly toxic substance when it appears in the extracellular milieu. To circumvent potential harmful effects of heme from hemoproteins released during physiological or pathological cell damage (such as hemolysis...... and rhabdomyolysis), specific high capacity scavenging systems have evolved in the mammalian organism. Two major systems, which essentially function in a similar way by means of a circulating latent plasma carrier protein that upon ligand binding is recognized by a receptor, are represented by a) the hemoglobin...

  5. Pedo-geochemical baseline content levels and soil quality reference values of trace elements in soils from the Mediterranean (Castilla La Mancha, Spain)

    Science.gov (United States)

    Ballesta, Raimundo; Bueno, Paz; Rubi, Juan; Giménez, Rosario

    2010-12-01

    To evaluate trace element soil contamination, geochemical baseline contents and reference values need to be established. Pedo-geochemical baseline levels of trace elements in 72 soil samples of 24 soil profiles from the Mediterranean, Castilla La Mancha, are assessed and soil quality reference values are calculated. Reference value contents (in mg kg-1) were: Sc 50.8; V 123.2; Cr 113.4; Co 20.8; Ni 42.6; Cu 27.0; Zn 86.5; Ga 26.7; Ge 1.3; As 16.7; Se 1.4; Br 20.1; Rb 234.7; Sr 1868.4; Y 38.3; Zr 413.1; Nb 18.7; Mo 2.0; Ag 7.8; Cd 4.4; Sn 8.7; Sb 5.7; I 25.4; Cs 14.2; Ba 1049.3; La 348.4; Ce 97.9; Nd 40.1; Sm 10.7; Yb 4.2; Hf 10.0; Ta 4.0; W 5.5; Tl 2.3; Pb 44.2; Bi 2.2; Th 21.6; U 10.3. The contents obtained for some elements are below or close to the detection limit: Co, Ge, Se, Mo, Ag, Cd, Sb, Yb, Hf, Ta, W, Tl and Bi. The element content ranges (the maximum value minus the minimum value) are: Sc 55.0, V 196.0, Cr 346.0, Co 64.4, Ni 188.7, Cu 49.5, Zn 102.3, Ga 28.7, Ge 1.5, As 26.4, Se 0.9, Br 33.0 Rb 432.7, Sr 3372.6, Y 39.8, Zr 523.2, Nb 59.7, Mo 3.9, Ag 10.1, Cd 1.8, Sn 75.2, Sb 9.9, I 68.0, Cs 17.6, Ba 1394.9, La 51.3, Ce 93.5, Nd 52.5, Sm 11.2, Yb 4.2, Hf 11.3, Ta 6.3, W 5.2, Tl 2.1, Pb 96.4, Bi 3.0, Th 24.4, U 16.4 (in mg kg-1). The spatial distribution of the elements was affected mainly by the nature of the bedrock and by pedological processes. The upper limit of expected background variation for each trace element in the soil is documented, as is its range as a criterion for evaluating which sites may require decontamination.

  6. A Bivariate Mixture Model for Natural Antibody Levels to Human Papillomavirus Types 16 and 18: Baseline Estimates for Monitoring the Herd Effects of Immunization.

    Science.gov (United States)

    Vink, Margaretha A; Berkhof, Johannes; van de Kassteele, Jan; van Boven, Michiel; Bogaards, Johannes A

    2016-01-01

    Post-vaccine monitoring programs for human papillomavirus (HPV) have been introduced in many countries, but HPV serology is still an underutilized tool, partly owing to the weak antibody response to HPV infection. Changes in antibody levels among non-vaccinated individuals could be employed to monitor herd effects of immunization against HPV vaccine types 16 and 18, but inference requires an appropriate statistical model. The authors developed a four-component bivariate mixture model for jointly estimating vaccine-type seroprevalence from correlated antibody responses against HPV16 and -18 infections. This model takes account of the correlation between HPV16 and -18 antibody concentrations within subjects, caused e.g. by heterogeneity in exposure level and immune response. The model was fitted to HPV16 and -18 antibody concentrations as measured by a multiplex immunoassay in a large serological survey (3,875 females) carried out in the Netherlands in 2006/2007, before the introduction of mass immunization. Parameters were estimated by Bayesian analysis. We used the deviance information criterion for model selection; performance of the preferred model was assessed through simulation. Our analysis uncovered elevated antibody concentrations in doubly as compared to singly seropositive individuals, and a strong clustering of HPV16 and -18 seropositivity, particularly around the age of sexual debut. The bivariate model resulted in a more reliable classification of singly and doubly seropositive individuals than achieved by a combination of two univariate models, and suggested a higher pre-vaccine HPV16 seroprevalence than previously estimated. The bivariate mixture model provides valuable baseline estimates of vaccine-type seroprevalence and may prove useful in seroepidemiologic assessment of the herd effects of HPV vaccination. PMID:27537200

  7. A Bivariate Mixture Model for Natural Antibody Levels to Human Papillomavirus Types 16 and 18: Baseline Estimates for Monitoring the Herd Effects of Immunization

    Science.gov (United States)

    Vink, Margaretha A.; Berkhof, Johannes; van de Kassteele, Jan; van Boven, Michiel; Bogaards, Johannes A.

    2016-01-01

    Post-vaccine monitoring programs for human papillomavirus (HPV) have been introduced in many countries, but HPV serology is still an underutilized tool, partly owing to the weak antibody response to HPV infection. Changes in antibody levels among non-vaccinated individuals could be employed to monitor herd effects of immunization against HPV vaccine types 16 and 18, but inference requires an appropriate statistical model. The authors developed a four-component bivariate mixture model for jointly estimating vaccine-type seroprevalence from correlated antibody responses against HPV16 and -18 infections. This model takes account of the correlation between HPV16 and -18 antibody concentrations within subjects, caused e.g. by heterogeneity in exposure level and immune response. The model was fitted to HPV16 and -18 antibody concentrations as measured by a multiplex immunoassay in a large serological survey (3,875 females) carried out in the Netherlands in 2006/2007, before the introduction of mass immunization. Parameters were estimated by Bayesian analysis. We used the deviance information criterion for model selection; performance of the preferred model was assessed through simulation. Our analysis uncovered elevated antibody concentrations in doubly as compared to singly seropositive individuals, and a strong clustering of HPV16 and -18 seropositivity, particularly around the age of sexual debut. The bivariate model resulted in a more reliable classification of singly and doubly seropositive individuals than achieved by a combination of two univariate models, and suggested a higher pre-vaccine HPV16 seroprevalence than previously estimated. The bivariate mixture model provides valuable baseline estimates of vaccine-type seroprevalence and may prove useful in seroepidemiologic assessment of the herd effects of HPV vaccination. PMID:27537200

  8. Study on the Hemoglobin levels among the Tibetan pregnant women in rural Lhasa%拉萨市农村藏族孕妇血红蛋白水平调查

    Institute of Scientific and Technical Information of China (English)

    康轶君; 李芳珍; 党少农; 颜虹; 曾令霞; 程悦; 李强; 毕育学

    2014-01-01

    Objective To understand the level of hemoglobin (Hb) and determine the risk factors of Hb concentration among the Tibetan pregnant women in rural Lhasa.Methods Between August 2008 and May 2011,a hospital-based study was conducted among l 530 Tibetan pregnant women after getting their informed consent in agricultural and pastoral areas in maternity clinic or hospital in city,county and township level of Lhasa.Their blood samples were tested and related socio-demographic information was collected.Blood was collected on tip of finger and the Hb concentration was measured using B-Hemoglobin photometer.American Centers for Disease Control and Prevention(CDC) method was used to adjust the Hb measurements based on altitude for estimating the prevalence of anemia.Anemia was assessed according to WHO criteria.A hemoglobin concentration of less than 110 g/L in a pregnant woman was considered an indication of anemia.Results From August 2008 to May 2011,1 530 Tibetan pregnant women living at (3 716.4 ± 69.1) m above sea level in rural Lhasa were examined.The findings indicated that average Hb concentration was (122.0 ± 17.5) g/L; the Hb concentration of 1st,2nd and 3rd trimester were (125.5 ± 18.0),(122.5±16.9) and (120.6 ± 17.4) g/L,respectively.The Hb concentration of pregnant women decreased with the increase of gestational age(P <0.05).Based onCDC method and the adjusted Hb level,the prevalence of anemia in pregnant women were 85.6% (1 310/1 530),the prevalence of anemia of 1st,2nd,and 3rd trimester were 79.5% (206/259),86.1% (395/459)and 87.3% (698/800),respectively (P <0.05).The rate of moderate and severe anemia were 46.7% (612/1 310) among pregnant women.Multiple linear regression analysis showed that high Hb level was associated significantly with low family income status (β =2.74,95 % CI:1.73-3.74),no bad habits (drinking or smoking) (β =7.34,95 % CI:4.87-9.81) and fewer times of pregnancies (β =-1.62,95% CI:-2.93--0.31) after adjusting

  9. Establishing baseline levels of trace elements in blood and skin of bottlenose dolphins in Sarasota Bay, Florida: implications for non-invasive monitoring.

    Science.gov (United States)

    Bryan, Colleen E; Christopher, Steven J; Balmer, Brian C; Wells, Randall S

    2007-12-15

    Several major unusual mortality events occurring in recent years have increased the level of concern for the health of bottlenose dolphin populations along the United States Atlantic and Gulf of Mexico coasts. Trace element concentrations were examined in a population of free-ranging dolphins in Sarasota Bay, Florida, in order to develop a benchmark for future comparisons within and between populations. Whole blood (n=51) and skin (n=40) samples were collected through capture and release health assessment events during 2002-2004. Samples were analyzed for Al, V, Cr, Mn, Cu, Zn, As, Se, Rb, Sr, Mo, Cd, and Pb by inductively coupled plasma mass spectrometry (ICPMS) and Hg via atomic fluorescence spectrometry (AFS). Trace element concentrations (wet mass) in skin were 2 to 45 times greater than blood, except Cu was approximately 1.5 times higher in blood. Statistically strong correlations (pdolphins had significantly greater Hg concentrations in blood and skin and Pb concentrations in skin, relative to males. Calves exhibited significantly lower V, As, and Hg concentrations in blood and V and Hg concentrations in skin, relative to other age classes. Rubidium and Cu concentrations in skin were greatest in subadults and calves, respectively. In blood, V, Zn, and As concentrations were significantly greater in winter, relative to summer, and the opposite trend was observed for Rb and Sr concentrations. In skin, Cu and Zn concentrations were significantly greater in winter, relative to summer, and the opposite trend was observed for Mn, Rb, Cd, and Pb concentrations. The baseline concentrations and trends established in this study will serve as a benchmark for comparison and aid in sampling design for future monitoring of this population and other coastal bottlenose dolphin populations. PMID:17870146

  10. Analysis on correlation among the level of serum glycosylated hemoglobin, blood glucose and blood lipid in the elderly diabetes patients%老年糖尿病患者糖化血红蛋白、血糖及血脂的相关性分析

    Institute of Scientific and Technical Information of China (English)

    詹德尚

    2014-01-01

    Objective To investigate the relationships among the level of serum glycosylated hemoglobin, blood glucose and blood lipid in the elderly diabetes patients. Methods 112 elderly diabetes patients and 102 elderly healthy physical examination people in our hospital from September 2009 to March 2013 were selected as the study objects. The level of serum glycosylated hemoglobin, blood glucose and blood lipid in patients were detected, and the relationships among the level of serum glycosylated hemoglobin, fasting blood glucose, 2h postprandial blood glucose, blood lipid were compared. Results There were statistical difference in the level of serum glycosylated hemoglobin, blood glucose and blood lipid between the diabetes patients and control group(P < 0.05); The level of serum glycosylated hemoglobin and fasting blood glucose, 2h postprandial blood glucose and blood lipid had positive correlation(P < 0.05). Conclusion The level of serum glycosylated hemoglobin, blood glucose and blood lipid in the elderly diabetes patients are obviously higher than those in normal physical examination people, and the level rise of glycosylated hemoglobin and blood glucose level, blood lipid level in patients have a certain correlation, and the detection of serum glycated hemoglobin has an important significance for the evaluation of control effect of blood glucose and blood lipid.%目的:探讨老年糖尿病患者血清糖化血红蛋白、血糖以及血脂水平之间的关系。方法选择2009年9月~2013年3月期间,于我院接受治疗的112例老年糖尿病患者及102例老年健康体检者作为研究对象,检测患者血清糖化血红蛋白与血糖及血脂,比较糖化血红蛋白与患者空腹血糖、餐后2h血糖、血脂之间的关系。结果糖尿病患者糖化血红蛋白、血糖及血脂与对照组比较,差异有统计学意义(P<0.05);糖尿病患者糖化血红蛋白与空腹血糖、餐后2h血糖及血

  11. 糖化血红蛋白水平与晚期胃癌 FOLFOX 方案化疗的近期有效率相关性研究%Association of the Glycosylated Hemoglobin Levels with the Short-term Effect of FOLFOX Chemotherapy in Advanced Gastric Cancer

    Institute of Scientific and Technical Information of China (English)

    汪青; 郑磊贞; 朱美玲; 陈思宇; 章莉

    2015-01-01

    目的:研究晚期胃癌患者不同的糖化血红蛋白水平与化疗的近期有效率的相关性。方法对52例晚期胃癌患者进行回顾性分析,测定化疗前后的患者糖化血红蛋白水平的变化,观察该指标与近期有效率( RR)的相关性。结果全组52例患者均完成至少3个周期化疗,并行疗效评价。该组患者FOLFOX方案化疗的近期有效率为32.7%,晚期胃癌患者化疗后糖化血红蛋白水平与患者不同的病灶数目的差异有统计学意义(P=0.039)。晚期胃癌患者糖化血红蛋白的低、中、高水平组RR分别为9、6、2例,3组患者化疗的近期有效率比较,差异有统计学意义( P<0.05);低、中、高糖化血红蛋白水平组疾病控制率分别为89.4%(17/19)、76.1%(16/21)、41.6%(5/12),组间比较,差异均有统计学意义(P<0.05)。结论糖化血红蛋白水平较高的晚期胃癌患者近期有效率较差,糖化血红蛋白水平可能是潜在的化疗近期疗效预测指标之一。%Objective To explore the correlation of the different glycosylated hemoglobin levels with the short-term effect of chemo-therapy in advanced gastric cancer.Methods Past immunohistochemical data of 52 advanced gastric cancer patients after FOLFOX chemotherapy were analyzed retrospectively.According to the change of the glycosylated hemoglobin levels before and after chemotherapy, it could be observed the association of the glycosylated hemoglobin levels with the short-term effect of chemotherapy.Results The whole group of 52 patients had completed at least three cycles of chemotherapy, and then got completed efficacy evaluation.Recent response rate of the patients who got FOLFOX chemotherapy was 17 cases (32.7%).The glycosylated hemoglobin levels with the different number of metastasis ( P=0.036) were significantly different.Advanced gastric cancer patients with low, medium and high level

  12. 糖化血红蛋白水平与晚期胃癌 FOLFOX 方案化疗的近期有效率相关性研究%Association of the Glycosylated Hemoglobin Levels with the Short-term Effect of FOLFOX Chemotherapy in Advanced Gastric Cancer

    Institute of Scientific and Technical Information of China (English)

    汪青; 郑磊贞; 朱美玲; 陈思宇; 章莉

    2015-01-01

    Objective To explore the correlation of the different glycosylated hemoglobin levels with the short-term effect of chemo-therapy in advanced gastric cancer.Methods Past immunohistochemical data of 52 advanced gastric cancer patients after FOLFOX chemotherapy were analyzed retrospectively.According to the change of the glycosylated hemoglobin levels before and after chemotherapy, it could be observed the association of the glycosylated hemoglobin levels with the short-term effect of chemotherapy.Results The whole group of 52 patients had completed at least three cycles of chemotherapy, and then got completed efficacy evaluation.Recent response rate of the patients who got FOLFOX chemotherapy was 17 cases (32.7%).The glycosylated hemoglobin levels with the different number of metastasis ( P=0.036) were significantly different.Advanced gastric cancer patients with low, medium and high level expression of gly-cosylated hemoglobin group RR was 9, 6, 2 cases.The three groups of efficient comparative difference was statistically significant ( P<0.05).The low, medium and high glycosylated hemoglobin levels of disease control rates were 89.4% (17/19), 76.1% (16/21), 41.6%(5/12).The three groups of efficient comparative difference was statistically significant (P<0.05).Conclusion Advanced gastric cancer patients with high glycosylated hemoglobin levels were not sensitive to FOLFOX chemotherapy.The glycosylated hemoglobin levels may be a potential predictor of short-term effect in the chemotherapy.%目的:研究晚期胃癌患者不同的糖化血红蛋白水平与化疗的近期有效率的相关性。方法对52例晚期胃癌患者进行回顾性分析,测定化疗前后的患者糖化血红蛋白水平的变化,观察该指标与近期有效率( RR)的相关性。结果全组52例患者均完成至少3个周期化疗,并行疗效评价。该组患者FOLFOX方案化疗的近期有效率为32.7%,晚期胃癌患者化疗后糖化血红蛋白水平与患者

  13. Hemoglobin induces colon cancer cell proliferation by release of reactive oxygen species

    Institute of Scientific and Technical Information of China (English)

    Ryung-Ah Lee; Hyun-Ah Kim; Bo-Young Kang; Kwang-Ho Kim

    2006-01-01

    AIM: To study whether hemoglobin could amplify colon cancer cell proliferation via reactive oxygen species (ROS)production.METHODS: Colon cancer cell line HT-29 was grown in the conventional method using RPMI1640 media. The viability of the cells was measured using the colorimetric MTT [3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide] assay after adding hemoglobin. We determined reactive oxygen species levels to be indicators of oxidative stress in HT 29 cell lines with and without hemoglobin and/or 5-fluorouracil (5-FU), 5'-deoxy-5-fluorouridine (5-DFUR) using fluorometric dichlorofluorescin diacetate (DCFH-DA) assay.RESULTS: Cellular proliferation was increased with hemoglobin in a concentration-dependent manner. A significant increment on ROS levels was found in HT 29 cells following hemoglobin incubation. The cytotoxic effects of 5-FU and 5-DFUR were significantly blunted by administration of hemoglobin. There was a slight increase of peroxiredoxin 1, superoxide dismutase 1 concentration according to different hemoglobin concentrations.CONCLUSION: Hemoglobin has a cellular proliferative effect on HT-29 colon cancer cell line by production of ROS. Also, hemoglobin abates cytotoxic effects of chemotherapeutic agents such as 5-FU and 5-DFUR.

  14. Hemoglobin A1c Levels and risk of severe hypoglycemia in children and young adults with type 1 diabetes from Germany and Austria: a trend analysis in a cohort of 37,539 patients between 1995 and 2012.

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    Beate Karges

    2014-10-01

    Full Text Available BACKGROUND: Severe hypoglycemia is a major complication of insulin treatment in patients with type 1 diabetes, limiting full realization of glycemic control. It has been shown in the past that low levels of hemoglobin A1c (HbA1c, a marker of average plasma glucose, predict a high risk of severe hypoglycemia, but it is uncertain whether this association still exists. Based on advances in diabetes technology and pharmacotherapy, we hypothesized that the inverse association between severe hypoglycemia and HbA1c has decreased in recent years. METHODS AND FINDINGS: We analyzed data of 37,539 patients with type 1 diabetes (mean age ± standard deviation 14.4 ± 3.8 y, range 1-20 y from the DPV (Diabetes Patienten Verlaufsdokumentation Initiative diabetes cohort prospectively documented between January 1, 1995, and December 31, 2012. The DPV cohort covers an estimated proportion of >80% of all pediatric diabetes patients in Germany and Austria. Associations of severe hypoglycemia, hypoglycemic coma, and HbA1c levels were assessed by multivariable regression analysis. From 1995 to 2012, the relative risk (RR for severe hypoglycemia and coma per 1% HbA1c decrease declined from 1.28 (95% CI 1.19-1.37 to 1.05 (1.00-1.09 and from 1.39 (1.23-1.56 to 1.01 (0.93-1.10, respectively, corresponding to a risk reduction of 1.2% (95% CI 0.6-1.7, p<0.001 and 1.9% (0.8-2.9, p<0.001 each year, respectively. Risk reduction of severe hypoglycemia and coma was strongest in patients with HbA1c levels of 6.0%-6.9% (RR 0.96 and 0.90 each year and 7.0%-7.9% (RR 0.96 and 0.89 each year. From 1995 to 2012, glucose monitoring frequency and the use of insulin analogs and insulin pumps increased (p<0.001. Our study was not designed to investigate the effects of different treatment modalities on hypoglycemia risk. Limitations are that associations between diabetes education and physical activity and severe hypoglycemia were not addressed in this study. CONCLUSIONS: The previously

  15. Establishing baseline levels of trace elements in blood and skin of bottlenose dolphins in Sarasota Bay, Florida: Implications for non-invasive monitoring

    Energy Technology Data Exchange (ETDEWEB)

    Bryan, Colleen E. [National Institute of Standards and Technology, Hollings Marine Laboratory, 331 Fort Johnson Road, Charleston, South Carolina 29412 (United States); College of Charleston, Grice Marine Laboratory, 205 Fort Johnson Road, Charleston, South Carolina 29412 (United States)], E-mail: colleen.bryan@nist.gov; Christopher, Steven J. [National Institute of Standards and Technology, Hollings Marine Laboratory, 331 Fort Johnson Road, Charleston, South Carolina 29412 (United States); Balmer, Brian C.; Wells, Randall S. [Chicago Zoological Society c/o Mote Marine Laboratory, 1600 Ken Thompson Parkway, Sarasota, Florida 34236 (United States)

    2007-12-15

    Several major unusual mortality events occurring in recent years have increased the level of concern for the health of bottlenose dolphin populations along the United States Atlantic and Gulf of Mexico coasts. Trace element concentrations were examined in a population of free-ranging dolphins in Sarasota Bay, Florida, in order to develop a benchmark for future comparisons within and between populations. Whole blood (n = 51) and skin (n = 40) samples were collected through capture and release health assessment events during 2002-2004. Samples were analyzed for Al, V, Cr, Mn, Cu, Zn, As, Se, Rb, Sr, Mo, Cd, and Pb by inductively coupled plasma mass spectrometry (ICPMS) and Hg via atomic fluorescence spectrometry (AFS). Trace element concentrations (wet mass) in skin were 2 to 45 times greater than blood, except Cu was approximately 1.5 times higher in blood. Statistically strong correlations (p < 0.05) were found for V, As, Se, Rb, Sr, and Hg between blood and skin demonstrating that these tissues can be used as effective non-lethal monitoring tools. The strongest correlation was established for Hg (r = 0.9689) and concentrations in both blood and skin were above the threshold at which detrimental effects are observed in other vertebrate species. Female dolphins had significantly greater Hg concentrations in blood and skin and Pb concentrations in skin, relative to males. Calves exhibited significantly lower V, As, and Hg concentrations in blood and V and Hg concentrations in skin, relative to other age classes. Rubidium and Cu concentrations in skin were greatest in subadults and calves, respectively. In blood, V, Zn, and As concentrations were significantly greater in winter, relative to summer, and the opposite trend was observed for Rb and Sr concentrations. In skin, Cu and Zn concentrations were significantly greater in winter, relative to summer, and the opposite trend was observed for Mn, Rb, Cd, and Pb concentrations. The baseline concentrations and trends

  16. Establishing baseline levels of trace elements in blood and skin of bottlenose dolphins in Sarasota Bay, Florida: Implications for non-invasive monitoring

    International Nuclear Information System (INIS)

    Several major unusual mortality events occurring in recent years have increased the level of concern for the health of bottlenose dolphin populations along the United States Atlantic and Gulf of Mexico coasts. Trace element concentrations were examined in a population of free-ranging dolphins in Sarasota Bay, Florida, in order to develop a benchmark for future comparisons within and between populations. Whole blood (n = 51) and skin (n = 40) samples were collected through capture and release health assessment events during 2002-2004. Samples were analyzed for Al, V, Cr, Mn, Cu, Zn, As, Se, Rb, Sr, Mo, Cd, and Pb by inductively coupled plasma mass spectrometry (ICPMS) and Hg via atomic fluorescence spectrometry (AFS). Trace element concentrations (wet mass) in skin were 2 to 45 times greater than blood, except Cu was approximately 1.5 times higher in blood. Statistically strong correlations (p < 0.05) were found for V, As, Se, Rb, Sr, and Hg between blood and skin demonstrating that these tissues can be used as effective non-lethal monitoring tools. The strongest correlation was established for Hg (r = 0.9689) and concentrations in both blood and skin were above the threshold at which detrimental effects are observed in other vertebrate species. Female dolphins had significantly greater Hg concentrations in blood and skin and Pb concentrations in skin, relative to males. Calves exhibited significantly lower V, As, and Hg concentrations in blood and V and Hg concentrations in skin, relative to other age classes. Rubidium and Cu concentrations in skin were greatest in subadults and calves, respectively. In blood, V, Zn, and As concentrations were significantly greater in winter, relative to summer, and the opposite trend was observed for Rb and Sr concentrations. In skin, Cu and Zn concentrations were significantly greater in winter, relative to summer, and the opposite trend was observed for Mn, Rb, Cd, and Pb concentrations. The baseline concentrations and trends

  17. Investigation of glycosylated hemoglobin A1c levels in 10487 adults in Xiamen city%厦门地区10487名成人糖化血红蛋白水平调查分析

    Institute of Scientific and Technical Information of China (English)

    金宏伟; 傅建国; 王欣欣; 黄回滨

    2014-01-01

    Objective To study the distribution of glycosylated hemoglobin A1c (HbA1c) levels in Xiamen,and to investigate the relationship among HbA1c level and gender and age.Methods This was a cross-sectional study.10 487 cases of physical examination individuals in Zhongshan hospital from January 2013 to April 2014 were recruited.HbA1c was measured by ARKRAY HA-8180 automatic glycosylated hemoglobin analyzer (Japan).All data were collected for analyzing the HbA1c levels in different sex and age groups,exploring the rule between HbA1c and age,and comparing the different diagnostic criteria of diabetes mellitus(DM).Differences between the two groups were compared using the Independent Samples T Test.The measurement data of multiple group were compared with single factor analysis of variance (One way ANOVA),and the comparison between two groups was performed with LSD T test.Correlation between different variables was analyzed by Pearson correlation and linear regression.Results Focused on all the 10 487 cases and 9 436 cases with HbA1c < 6.5% physical examination population,the levels of HbA1c were positively correlated with age,the linear regression equations were HbA1c (%) =0.021 2 × age (years) + 4.811 9 (r =0.348 9,P=0.000) and HbA1c(%) =0.010 9 × age (years) + 5.0944 (r=0.4257,P =0.000)respectively; HbA1c levels in male physical examination population were significantly higher than those in females (P < 0.001),moreover,in different gender groups,HbA1c level had gradually risen with increasing age.Patients at risk for diabetes (prediabetes,39.60%) diagnosed by the criteria of 2010 American Diabetes Association (ADA) were 2.55 times more than those diagnosed by the criteria of international expert committee (15.55%).Conclusions The HbA1c level is related to age and gender in Xiamen city.It is necessary to establish different reference intervals and DM diagnostic cut-off point.The criteria of 2010 ADA can identify more high-risk DM individuals,with is

  18. Correlation between glycosylated hemoglobin level and coronary SYNTAX score in patients with coronary heart disease%冠心病患者糖化血红蛋白水平与冠脉SYNTAX评分的相关性

    Institute of Scientific and Technical Information of China (English)

    刘天民; 周芳; 肖宏凯; 黄茵; 刘小慧; 林岫芳

    2014-01-01

    目的:探讨冠心病患者糖化血红蛋白水平与冠脉病变严重程度的相关性。方法:选择心内科住院并行冠状动脉造影检查的515例患者资料进行分析,按照冠脉造影结果分为冠心病组(370例)和冠脉造影结果正常组(145例);冠心病组患者再按糖化血红蛋白水平分为HbA1 c<7%组(282例)和 HbA1 c≥7%组(88例),按SYNTAX评分分为SYNTAX评分低危组(1-22分,181例)、中危组(23-32分,125例)及高危组(≥33分,64例)三个亚组,分析比较各组间糖化血红蛋白水平的差异。结果:HbA1 c≥7%组冠心病患者的冠脉SYNTAX 评分显著高于HbA1 c<7%组[(25.6±9.97)分比(20.92±10.26)分, P<0.01]。SYNTAX评分高危组和中危组HbA1c水平显著高于冠脉造影正常组、低危组[(8.74±0.83)分、(7.74±0.62)分比(5.12±0.49)分比(5.68±0.59)分],且低危组也显著高于正常组(P均<0.01)。Pearson相关分析显示 HbA1c水平与SYNTAX评分呈正相关(r=0.764,P<0.01)。结论:糖化血红蛋白水平与冠脉病变严重程度呈正相关,SYNTAX评分高者糖化血红蛋白水平明显高于SYNTAX评分低者。%Objective:To explore the correlation between glycosylated hemoglobin (HbA1c) level and severity of cor-onary artery disease in patients with coronary heart disease (CHD) .Methods :Clinical data of 515 patients ,who hospitalized in department of cardiology and received coronary angiography (CAG ) examination ,were analyzed . According to CAG results ,they were divided into CHD group (n=370) and normal CAG group (n=145);accord-ing to HbA1c level ,CHD patients were further divided into HbA1c<7% group (n=282) and HbA1c≥7% group (n=88) ,according to SYNTAX score ,they were divided into SYNTAX score low risk group (n=181 ,1~22 scores) , intermediate risk group (n=125 ,23~32 scores) and high risk group (n=64 ,≥33 scores) .HbA1c level was com-pared and

  19. Clinical Significance of Detecting Levels of Glycosylated Hemoglobin and Uric Acid in Patients with Impaired Gglucose Tolerance%糖耐量异常患者糖化血红蛋白、血清尿酸水平改变意义的分析

    Institute of Scientific and Technical Information of China (English)

    李珊

    2014-01-01

    目的:探讨糖耐量异常患者糖化血红蛋白(HbA1c)及血清尿酸(UA)水平变化及临床意义。方法以62例糖耐量异常患者及40例健康体检者为研究对象,对其体内HbA1c和血清UA水平进行检测与分析。结果糖耐量异常组患者FBG、2hPBG等血糖指标水平及HbA1c、血清UA水平均显著高于对照组,差异均具有统计学意义(P<0.05);糖耐量异常患者HbA1c与血清UA水平的相关性回归分析表明, HbA1c与血清UA水平呈显著正相关(P<0.05)。结论检测糖耐量异常患者体内HbA1c和血清UA水平对糖耐量异常的诊疗具有重要的临床意义。%Objective To explore the clinical significance of detecting the levels of glycosylated hemoglobin and uric acid in patients with impaired glucose tolerance (IGT). Methods 62 patients with IGT in IGT group and 40 healthy volunteers in control group were as the research objects, the levels of glycosylated hemoglobin and uric acid were detected and analyzed. Results The levels of FBG, 2hPBG, glycosylated hemoglobin and uric acid of patients in the IGT group were signiifcantly higher than those of healthy volunteers in the control group, the differences were statistically signiifcant (P<0.05), and the levels of glycosylated hemoglobin was positively correlated with the serum level of uric acid (P<0.05). Conclusion The detection of levels of glycosylated hemoglobin and uric acid has important clinical signiifcance in patients with IGT in the diagnosis and treatment.

  20. A tri state mechanism for oxygen release in fish hemoglobin: Using Barbus sharpeyi as a model

    Directory of Open Access Journals (Sweden)

    Mohammad Reza Dayer

    2014-06-01

    Full Text Available Hemoglobin is a porphyrin containing protein with an a2b2 tetrameric structure and like other porphyrin compounds shows spectral behavior of species specific characteristics. Researchers tend to relate bands in the hemoglobin spectra to certain structural and/or functional features. Given the fact that hemoglobin is the main oxygen carrier in animals functioning through the Oxy«Deoxy equilibrium, the determination of oxy and deoxy conformations of hemoglobins of different animals may shed light on their oxygen binding properties. Absorption spectra at 280 and 373nm have been widely used to quantitate the formation of hemoglobin deoxy conformation. In the present work, however, we used an optical density ratio of OD373/OD280 as an index for deoxy formation. This ratio was determined for Barbus sharpeyi and human hemoglobins at different SDS concentrations, pH levels and temperatures to compare them from a structure-function point of view. Our data showed that under low concentrations of SDS (Barbus sharpeyi hemoglobin folds in a tri-state pattern while human hemoglobin folds through a two-state phenomenon. This finding indicates that in contrast to those of other non aquatic animals, the hemoglobin of Barbus sharpeyi has a loosely folded tetrameric structure with remarkably more oxygen affinity

  1. Role of baseline HIV-1 DNA level in highly-experienced patients receiving raltegravir, etravirine and darunavir/ritonavir regimen (ANRS139 TRIO trial.

    Directory of Open Access Journals (Sweden)

    Charlotte Charpentier

    Full Text Available OBJECTIVE: In the ANRS 139 TRIO trial, the use of 3 new active drugs (raltegravir, etravirine, and darunavir/ritonavir, resulted in a potent and sustained inhibition of viral replication in multidrug-resistant treatment-experienced patients. The aim of this virological sub-study of the ANRS 139 TRIO trial was to assess: (i the evolution of HIV-1 DNA over the first year; and (ii the association between baseline HIV-1 DNA and virological outcome. METHODS: Among the 103 HIV-1-infected patients included in the ANRS-139 TRIO trial, HIV-1 DNA specimens were available for 92, 84, 88, and 83 patients at Week (W0, W12, W24, and W48, respectively. Quantification of total HIV-1 DNA was performed by using the commercial kit "Generic HIV DNA Cell" (Biocentric, Bandol, France. RESULTS: Baseline median HIV-1 DNA of patients displaying virological success (n= 61, viral blip (n= 20, and virological failure (n = 11 were 2.34 log(10 copies/10(6 PBMC (IQR= 2.15-2.66, 2.42 (IQR = 2.12-2.48, and 2.68 (IQR= 2.46-2.83, respectively. Although not statistically significant, patients exhibiting virological success or viral blip had a tendency to display lower baseline HIV-1 DNA than patients experiencing virological failure (P = 0.06. Median decrease of HIV-1 DNA between baseline and W48 was -0.13 log(10 copies/10(6 PBMC (IQR = -0.34 to +0.10, mainly explained by the evolution from W0 to W4. No more changes were observed in the W4-W48 period. CONCLUSIONS: In highly-experienced multidrug-resistant patients, HIV-1 DNA slightly decreased during the first month and then remained stable during the first year of highly potent antiretroviral regimen. In this population, baseline HIV-1 DNA might help to better predict the virological response and to tailor clinical therapeutic management as more aggressive therapeutic choices in patients with higher baseline HIV-1 DNA.

  2. Hemoglobin is essential for normal growth of Arabidopsis organs

    DEFF Research Database (Denmark)

    Hebelstrup, Kim Henrik; Hunt, Peter; Dennis, Elizabeth;

    2006-01-01

    lines are viable but show a mutant phenotype affecting the regions where AHb1 is expressed. Arabidopsis lines with an insertional knockout or overexpression of AHb2, a class II 3-on-3 hemoglobin, were generated. Seedlings overexpressing AHb2 show enhanced survival of hypoxic stress. The AHb2 knockout...... lines develop normally. However, when AHb2 knockout is combined with AHb1 silencing, seedlings die at an early vegetative stage suggesting that the two 3-on-3 hemoglobins, AHb1 and AHb2, together play an essential role for normal development of Arabidopsis seedlings. In conclusion, these results...... suggests that 3-on-3 hemoglobins apart from a role in hypoxic stress play a general role under non-stressed conditions where they are essential for normal development by controlling the level of NO which tends to accumulate in floral buds and leaf hydathodes of plants...

  3. Hemoglobin as an important prognostic factor in concurrent chemoradiotherapy for locally advanced carcinoma of the cervix

    International Nuclear Information System (INIS)

    The objective of this study was to examine a possible association of hemoglobin with clinical outcome in patients with locally advanced squamous cell carcinoma of the cervix who were treated with concurrent chemoradiotherapy (CCRT). Seventy-five patients with Stage IB to IVA disease who were treated with CCRT were reviewed retrospectively. The mean age was 49.8 years. In the treatment, standard radiotherapy was performed accompanied by concomitant chemotherapy using cisplatin. Pre-treatment hemoglobin was defined as the earliest hemoglobin level prior to the initiation of treatment. Weekly nadir hemoglobin levels throughout treatment were averaged and used as average weekly nadir hemoglobin during treatment (AWNHg). The mean follow-up time was 28.6 months. The mean pre-treatment hemoglobin of 11.6 g/dL was significantly reduced to the mean AWNHg of 9.9 g/dL. The levels of pre-treatment hemoglobin and AWNHg were significantly associated with tumor response to treatment. The 5-year cumulative disease-free survival and overall survival rates for all 75 patients were 67.8% and 75.3%, respectively. Multivariate statistical analysis revealed that AWNHg (≥9.0 versus <9.0 g/dL) was an independent prognostic factor for overall survival (p=0.038), but pre-treatment hemoglobin was not a significant factor. AWNHg was one of the most powerful independent predictors of overall survival in patients undergoing CCRT for locally advanced squamous cell carcinoma of the cervix. (author)

  4. Glycated Hemoglobin Measurement and Prediction of Cardiovascular Disease

    DEFF Research Database (Denmark)

    Di Angelantonio, Emanuele; Gao, Pei; Khan, Hassan;

    2014-01-01

    IMPORTANCE: The value of measuring levels of glycated hemoglobin (HbA1c) for the prediction of first cardiovascular events is uncertain. OBJECTIVE: To determine whether adding information on HbA1c values to conventional cardiovascular risk factors is associated with improvement in prediction of c...

  5. Glycated Hemoglobin Measurement and Prediction of Cardiovascular Disease

    NARCIS (Netherlands)

    Di Angelantonio, Emanuele; Gao, Pei; Khan, Hassan; Butterworth, Adam S.; Wormser, David; Kaptoge, Stephen; Seshasai, Sreenivasa Rao Kondapally; Thompson, Alex; Sarwar, Nadeem; Willeit, Peter; Ridker, Paul M.; Barr, Elizabeth L. M.; Khaw, Kay-Tee; Psaty, Bruce M.; Brenner, Hermann; Balkau, Beverley; Dekker, Jacqueline M.; Lawlor, Debbie A.; Daimon, Makoto; Willeit, Johann; Njolstad, Inger; Nissinen, Aulikki; Brunner, Eric J.; Kuller, Lewis H.; Price, Jackie F.; Sundstrom, Johan; Knuiman, Matthew W.; Feskens, Edith J. M.; Verschuren, W. M. M.; Wald, Nicholas; Bakker, Stephan J. L.; Whincup, Peter H.; Ford, Ian; Goldbourt, Uri; Gomez-de-la-Camara, Agustin; Gallacher, John; Simons, Leon A.; Rosengren, Annika; Sutherland, Susan E.; Bjorkelund, Cecilia; Blazer, Dan G.; Wassertheil-Smoller, Sylvia; Onat, Altan; Ibanez, Alejandro Marin; Casiglia, Edoardo; Jukema, J. Wouter; Simpson, Lara M.; Giampaoli, Simona; Nordestgaard, Borge G.; Selmer, Randi; Wennberg, Patrik; Kauhanen, Jussi; Salonen, Jukka T.; Dankner, Rachel; Barrett-Connor, Elizabeth; Kavousi, Maryam; Gudnason, Vilmundur; Evans, Denis; Wallace, Robert B.; Cushman, Mary; D'Agostino, Ralph B.; Umans, Jason G.; Kiyohara, Yutaka; Nakagawa, Hidaeki; Sato, Shinichi; Gillum, Richard F.; Folsom, Aaron R.; van der Schouw, Yvonne T.; Moons, Karel G.; Griffin, Simon J.; Sattar, Naveed; Wareham, Nicholas J.; Selvin, Elizabeth; Thompson, Simon G.; Danesh, John

    2014-01-01

    IMPORTANCE The value of measuring levels of glycated hemoglobin (HbA(1c)) for the prediction of first cardiovascular events is uncertain. OBJECTIVE To determine whether adding information on HbA(1c) values to conventional cardiovascular risk factors is associated with improvement in prediction of ca

  6. Baseline Hepatitis B Virus DNA Level is a Promising Factor for Predicting the 3rd Month Virological Response to Entecavir Therapy: A Study of Strict Defined Hepatitis B virus Induced Cirrhosis

    Institute of Scientific and Technical Information of China (English)

    Yang Xu; Xiao-Ning Wu; Yi-Wen Shi; Wei Wei; Ai-Ting Yang; Ya-Meng Sun; Wen-Shan Zhao

    2015-01-01

    Background:Cirrhosis is a common complication of chronic hepatitis B.It remains unclear if viral and biochemical parameters at baseline affect virological response to entecavir and therefore warrant investigation.In the present study,we aimed to evaluate the efficacy of entecavir therapy by monitoring virological response at the end of the 3rd month of treatment and try to figure out whether baseline factors could help predict it in a cohort of hepatitis B virus (HBV) compensated cirrhosis patients and to determine the cut-off value of a predicting parameter.Methods:A total of 91 nucleos(t)ide-na(i)ve patients with HBV induced cirrhosis (compensatory stage) were enrolled in a prospective cohort.HBV DNA and alanine aminotransferase (ALT) were tested at baseline and monitored every 3-6 months after starting therapy.Results:Of all 91 patients,the median follow-up time was 12 (9-24) months.Overall,64 patients (70.3%) achieved virological response in the 3rd month.Univariate analysis showed that the 3rd month virological response can be predicted by baseline HBV DNA levels (P < 0.001,odds ratio [OR]:2.13,95% confidence interval [CI]:1.44-3.15),ALT value (P =0.023,OR:1.0 1,95% CI:1.00-1.01) and hepatitis B e antigen (HBeAg) negativity (P=0.016,OR:0.30,95% CI:0.11-0.80).Multiple regression analysis showed baseline HBV DNA level was the only parameter related to full virological response.Higher baseline HBV DNA strata indicated a higher probability that HBV DNA remains detectable at the 3rd month (P =0.001).Area under receiver operating characteristic curve for determining the 3rd month virological response by baseline HBV DNA was 77.6% (95% CI:66.7-85.2%),with a best cut-off value of 5.8 log10.Conclusions:Baseline HBV DNA,HBeAg negativity,and ALT were independent factors contributing to virological response at the 3rd month.Further,multiple regression showed that HBV DNA level was the only parameter predicting full virological response as early as the 3rd

  7. 慢性心力衰竭患者血红蛋白水平与心肾功能的相关性%Relationship among hemoglobin level and cardiac function, renal function in patients with chronic heart failure

    Institute of Scientific and Technical Information of China (English)

    张艳

    2013-01-01

    目的:探讨慢性心力衰竭(CHF)患者血红蛋白(Hb)水平与其心功能、肾功能的关系.方法:150例CHF男性患者按照心功能分为三组:NYHA Ⅱ级组44例、Ⅲ级组49例,Ⅳ级组57例,并随机抽选30例健康体检患者为健康对照组,分析各组心功能指标[左室舒张末期内径(LVEDd),左室射血分数(LVEF),N末端脑利钠肽前体(NT-proBNP)],肾功能指标[血清肌酐(SCr),肾小球率过滤(GFR)],血液学指标[Hb,红细胞(RBC),血红细胞比容(HCT)];根据CHF患者贫血情况分为贫血组(61例)和非贫血组(89例),并对两组上述指标进行比较.结果:CHF各组贫血发生率均明显高于健康对照组,且随着心衰等级的增加而明显升高(P<0.05);与健康对照组比较,CHF各组心功能各项指标(LVEDd,LVEF,NT-proBNP),肾功能指标(SCr,GFR),血液学指标(Hb,RBC,HCT)均有明显恶化,且随着心衰等级增加而明显加重(P均<0.05);与CHF非贫血组比较,CHF贫血组SCr[(89.78±44.79) mmol/L比(78.79±45.02) mmol/L]、GFR[(86.13±25.42)ml· min-1(1.73m)-1比(67.99±32.12) ml· min-1(1.73m)-1]、LVEF[(55.79±11.34)%比(45.65±12.03)%]水平明显降低,LVEDd[(49.89±8.93) mm比(56.45±11.23) mm]明显增大,NT-proBNP[(945.27±1249.76) pg/ml比(3884.23±2790.42) pg/ml]水平明显升高(P<0.05);线性相关分析显示Hb与LVEDd、NT-proBNP呈负相关(r=-0.346,-0.547,P均<0.05),与LVEF、GFR呈正相关(r=0.453,0.338;P均<0.05).结论:慢性心力衰竭患者血红蛋白下降会显著降低心、肾功能.%Objective:To explore the relationship among hemoglobin (Hb) level and cardiac function,renal function in patients with chronic heart failure (CHF).Methods:According to NYHA cardiac function class,a total of 150 male CHF patients were divided into NYHA class Ⅱ group (n =44),NYHA class Ⅲ group (n =49) and NYHA class Ⅳ group (n =57).Another 30 healthy subjects were randomly selected as healthy control group.Indexes of cardiac function [left

  8. Hemoglobin variant (hemoglobin Aalborg) mimicking interstitial pulmonary disease.

    Science.gov (United States)

    Panou, Vasiliki; Jensen, Peter-Diedrich Mathias; Pedersen, Jan Freddy; Thomsen, Lars Pilegaard; Weinreich, Ulla Møller

    2014-01-01

    Hemoglobin Aalborg is a moderately unstable hemoglobin variant with no affiliation to serious hematological abnormality or major clinical symptoms under normal circumstances. Our index person was a healthy woman of 58, not previously diagnosed with hemoglobinopathy Aalborg, who developed acute respiratory failure after a routine cholecystectomy. Initially she was suspected of idiopathic interstitial lung disease, yet a series of tests uncovered various abnormal physiological parameters and set the diagnosis of hemoglobinopathy Aalborg. This led us to examine a group of the index person's relatives known with hemoglobinopathy Aalborg in order to study whether the same physiological abnormalities would be reencountered. They were all subjected to spirometry and body plethysmography, six-minute walking test, pulse oximetry, and arterial blood gas samples before and after the walking test. The entire study population presented the same physiological anomalies: reduction in diffusion capacity, and abnormalities in P(a)O2 and p50 values; the latter could not be presented by the arterial blood gas analyzer; furthermore there was concordance between pulse oximetry and arterial blood gas samples regarding saturation. These data suggest that, based upon the above mentioned anomalies in physiological parameters, the diagnosis of hemoglobinopathy Aalborg should be considered.

  9. Hemoglobin Variant (Hemoglobin Aalborg Mimicking Interstitial Pulmonary Disease

    Directory of Open Access Journals (Sweden)

    Vasiliki Panou

    2014-01-01

    Full Text Available Hemoglobin Aalborg is a moderately unstable hemoglobin variant with no affiliation to serious hematological abnormality or major clinical symptoms under normal circumstances. Our index person was a healthy woman of 58, not previously diagnosed with hemoglobinopathy Aalborg, who developed acute respiratory failure after a routine cholecystectomy. Initially she was suspected of idiopathic interstitial lung disease, yet a series of tests uncovered various abnormal physiological parameters and set the diagnosis of hemoglobinopathy Aalborg. This led us to examine a group of the index person’s relatives known with hemoglobinopathy Aalborg in order to study whether the same physiological abnormalities would be reencountered. They were all subjected to spirometry and body plethysmography, six-minute walking test, pulse oximetry, and arterial blood gas samples before and after the walking test. The entire study population presented the same physiological anomalies: reduction in diffusion capacity, and abnormalities in PaO2 and p50 values; the latter could not be presented by the arterial blood gas analyzer; furthermore there was concordance between pulse oximetry and arterial blood gas samples regarding saturation. These data suggest that, based upon the above mentioned anomalies in physiological parameters, the diagnosis of hemoglobinopathy Aalborg should be considered.

  10. Níveis de hemoglobina glicosilada e anomalias cardíacas em fetos de mães com diabetes mellitus Glycosylated hemoglobin levels and cardiac abnormalities in fetuses of diabetic mothers

    Directory of Open Access Journals (Sweden)

    Ivo Behle

    1998-06-01

    Full Text Available Avaliou-se, prospectivamente, a existência de relação entre o controle glicêmico materno, na primeira metade da gestação, com a ocorrência de anomalias cardíacas fetais, em gestantes com diabetes mellitus. O nível da hemoglobina glicosilada (HbA1c foi determinado em 127 grávidas, por ocasião da primeira visita pré-natal. Nove eram portadoras de diabetes do tipo I, 77 do tipo II e 41 de Diabetes mellitus gestacional (DMG. Todas foram submetidas a ecocardiografia fetal detalhada na 28ª (±4,1 semana da gestação. Em 31 dos 127 fetos (24,4% foram detectadas anomalias cardíacas. Em 10 (7,87%, foram diagnosticadas anomalias cardíacas estruturais e, em 21 (16,53%, miocardiopatia hipertrófica como anomalia cardíaca isolada. A média das dosagens de HbA1c no grupo de gestantes sem anomalias cardíacas (5,6% foi estatisticamente diferente da média das HbA1c do grupo com anomalias (10,1% (pWe analyze prospectively the existence of a relationship between the mother's glycemic control, in the first half of pregnancy, and the occurrence of abnormal fetal cardiac abnormalities, in pregnant women with diabetes mellitus. In 127 pregnant women, the level of glycosylated hemoglobin was determined on the first visit during prenatal care. Nine patients had type I diabetes, 77 type II and 41 gestational diabetes mellitus (GDM. All mothers were submitted to detailed fetal echocardiography, during the 28th ± 4.127 week of gestation. In 31 (24.4% of the 127 fetuses cardiac anomalies were detected. In 10 (7.87% an isolated cardiac anomaly was identified. Mean HbA1c in the group of pregnant women without cardiac anomalies (5.64% was statistically different from the group with anomalies (10.14% (p<0.0001. The receiver-operator characteristic, representing the balance between sensitivity (92.83% and specificity (98.92% in the diagnosis of structural cardiac abnormalities, showed a cut-off point at the 7.5% HbA1c level. In nine of ten fetuses with

  11. Hemoglobin variants: biochemical properties and clinical correlates.

    Science.gov (United States)

    Thom, Christopher S; Dickson, Claire F; Gell, David A; Weiss, Mitchell J

    2013-03-01

    Diseases affecting hemoglobin synthesis and function are extremely common worldwide. More than 1000 naturally occurring human hemoglobin variants with single amino acid substitutions throughout the molecule have been discovered, mainly through their clinical and/or laboratory manifestations. These variants alter hemoglobin structure and biochemical properties with physiological effects ranging from insignificant to severe. Studies of these mutations in patients and in the laboratory have produced a wealth of information on hemoglobin biochemistry and biology with significant implications for hematology practice. More generally, landmark studies of hemoglobin performed over the past 60 years have established important paradigms for the disciplines of structural biology, genetics, biochemistry, and medicine. Here we review the major classes of hemoglobin variants, emphasizing general concepts and illustrative examples.

  12. Hemoglobin Labeled by Radioactive Lysine

    Science.gov (United States)

    Bale, W. F.; Yuile, C. L.; DeLaVergne, L.; Miller, L. L.; Whipple, G. H.

    1949-12-08

    This paper reports on the utilization of tagged epsilon carbon of DL-lysine by a dog both anemic and hypoproteinemic due to repeated bleeding plus a diet low in protein. The experiment extended over period of 234 days, a time sufficient to indicate an erythrocyte life span of at least 115 days based upon the rate of replacement of labeled red cell proteins. The proteins of broken down red cells seem not to be used with any great preference for the synthesis of new hemoglobin.

  13. Randomised β-carotene supplementation and incidence of cancer and cardiovascular disease in women: is the association modified by baseline plasma level?

    OpenAIRE

    Lee, I-M; Cook, N R; Manson, J E; Buring, J. E.

    2002-01-01

    In a nested case-control study of 513 women with cancer; 130 with cardiovascular disease and equal numbers of controls, we found no effect of randomised beta-carotene on risk of cancer or cardiovascular disease within any quartile of baseline plasma beta-carotene, nor was there a trend across quartiles (P for trend 0.15 and 0.62, respectively). British Journal of Cancer (2002) 86, 698–701. DOI: 10.1038/sj/bjc/6600147 www.bjcancer.com © 2002 Cancer Research UK

  14. 糖化血红蛋白与糖调节受损血糖水平相关性的研究%Correlation of glycosylated hemoglobin and blood glucose levels of impaired glucose regulation

    Institute of Scientific and Technical Information of China (English)

    孟作龙; 李坤山; 方莹; 周颖; 赵艳; 韩月香; 王晓敏

    2010-01-01

    Objective To investigate the correlation of glycosylated hemoglobin (HbAlc) and impaired glucose regulation(IGR). Methods Seventy-five g oral glucose tolerance test(OGTT)and HbAlc levels were carried out among our hospital staffs. The subjects were determined fasting plasma glucose (FPG), oral glucose 2 h plasma glucose(2 h PG)and HbAlc, plasma glucose levels and HbAlc levels were determined by glucose oxidase method and high performance liquid chromatogram ( HPLC). The inclusion criteria of subjects was FPG<7.0 mmol/L and 2 h PG<11.1 mmol/L, without diabetes, hemoglobin disease, hepatopathy and renopathy. The selected object contained 726 subjects (197 male, 529 female, mean age (39±10)), including NGT 636 subjects(87.6% ) , IGR 90 subjects( 12.4% ). The diagnostic criteria of IGR selected 1999 WHO diagnostic criteria for diabetes. χ~2 test was used for rate comparison. Pearson correlation analysis was used for bivariate analysis selected. Results (1)2.3% occurred IGR among HbAlc≤5.7% subjects, while 89. 3% occurred IGR among HbAlc≥5.8% subjects. When HbAlc ≥5.8% ,the sensibility, specificity, PV + , and PV - were 83% , 99% , 0.89, 0.98, respectively. (2) Between HbAlc level 5.8% group and HbAlc 5.7% group, the difference of prevalence of the OGTT diagnosed IFG,IGT,IGR had statistically significance(χ~2 value was 10. 077,22. 219 and 27. 780, P<0.01 or P<0.001), (3) HbAlc levels was significant positive correlation with the prevalence of IFG,IGT,IGR(rralue was 0.957,0.928 and 0.936, all P<0.01).Conclusions (1) Compared with OGTT, HbAlc had consistency for predicting IGR, and the optimal critical value of HbAlc with OGTT diagnosed IGR was 5.8%. (2) HbAlc levels was significant positive correlation with the blood sugar level of the OGTT diagnosed IFG, IGT, IGR, and when HbAlc =5.8%, its relevance is most closely. The present study suggests that the HbAlc ≥5.8% patients should be examined OGTT in order to identify whether the IGR.%目的 探讨研究糖化

  15. Exploring the Relationships between Hemoglobin, the Endothelium and Vascular Health in Patients with Chronic Kidney Disease

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    Catherine Weber

    2011-11-01

    Full Text Available Background/Aims: The ideal hemoglobin target in chronic kidney disease remains unknown. Ultimately, individualized targets may depend upon the properties of the patient’s endothelial and vascular milieu, and thus the complex relationships between these factors need to be further explored. Methods: Forty-six patients with a glomerular filtration rate (GFR 2 or on renal replacement therapy underwent measurement of hemoglobin, endothelial microparticles (EMPs and aortic pulse wave velocity (PWV at 0, 3 and 6 months. In addition, a number of inflammatory, cardiac and vascular biomarkers were measured at baseline. Results: No correlation was observed between baseline values of PWV and EMPs, PWV and hemoglobin, or hemoglobin and EMPs in the overall cohort. When stratified by CKD status, a positive correlation was observed between PWV and EMP CD41–/CD144+ in patients with GFR 2 only (r = 0.54, p = 0.01. Asymmetric dimethylarginine correlated with baseline PWV (r = 0.27, p = 0.07, and remained significantly correlated with the 3- and 6-month PWV measurement. Conclusions: In this small heterogeneous cohort of dialysis and non-dialysis patients, we were unable to describe a physiologic link between anemia, endothelial dysfunction and arterial stiffness.

  16. Efficacy of extended release quetiapine fumarate monotherapy in elderly patients with major depressive disorder: secondary analyses in subgroups of patients according to baseline anxiety, sleep disturbance, and pain levels.

    Science.gov (United States)

    Montgomery, Stuart A; Altamura, A Carlo; Katila, Heikki; Datto, Catherine; Szamosi, Johan; Eriksson, Hans

    2014-03-01

    This study evaluated extended release quetiapine fumarate (quetiapine XR) monotherapy in elderly patients with major depressive disorder (MDD) according to baseline levels of anxiety, sleep disturbance, and pain. Post-hoc analyses of data from an 11-week (9-week randomized-treatment, 2-week post-treatment phase), double-blind, placebo-controlled study of quetiapine XR (50-300 mg/day) monotherapy in elderly (≥66 years) patients (n=338) with MDD were carried out. Outcomes included randomization to week 9 change in Montgomery Åsberg Depression Rating Scale (MADRS) score and week 9 response (≥50% MADRS score reduction) rates. Post-hoc analyses were carried out to assess subgroups of patients with MDD according to baseline levels in terms of the following: higher or lower anxiety (Hamilton Rating Scale for Anxiety total score≥20 or Depression sleep disturbance factor (items 4+5+6) score≥5 or depressive symptoms in elderly patients with MDD, irrespective of baseline levels of anxiety, sleep disturbance, and pain.

  17. Patterns of glycemic control using glycosylated hemoglobin in diabetics

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    Arunpreet Singh Kahlon

    2011-01-01

    Full Text Available Aim : Till now estimation of blood glucose is the highly effective method for diagnosing diabetes mellitus but it provides a short-term picture of control. More evidence is required to prove that plasma glucose and glycosylated hemoglobin levels together gives a better estimate of glycemic control and compliance with treatment. Indian diabetes risk score (IDRS is a simplified screening tool for identifying undiagnosed diabetic subjects, requires minimum time, and effort and can help to considerably reduce the costs of screening. Objective : To study patterns of glycemic control using glycosylated hemoglobin in diabetic patients. To find out correlation between levels of plasma glucose and glycosylated hemoglobin in diabetics and to calculate IDRS of the study population. Materials and Methods : A cross sectional study was conducted among 300 known diabetic patients attending outpatient department of a rural medical college in Haryana, India. Following standard procedures and protocols FPG and glycosylated hemoglobin were measured to find out a pattern of glycemic control in them after taking their written and informed consent. A correlation between the levels of glycosylated hemoglobin and fasting blood glucose was also calculated. These patients were made to fill a performa and their demographic and clinical risk factors were noted and based on this, their IDRS was calculated. This was done to validate the IDRS in Indian rural population. Results : Fifty-two percent of the population had fasting plasma glucose level between 125-150 mg/dl, 21% had this level between 151-175 mg/dl. Thirteen percent of the study subjects had HbA1C between 6.5-7.5, more than half (57.3% had this value between 7.5-8.5, 12% and 18% had values between 8.5-9.5 and 9.5-10.5, respectively. Twelve percent of the participants had HbA1C level higher than 10.5. Correlation of fasting plasma glucose level and HbA1C was also studied and found that correlation coefficient came

  18. Manipulation of hemoglobin expression affects Arabidopsis shoot organogenesis.

    Science.gov (United States)

    Wang, Yaping; Elhiti, Mohamed; Hebelstrup, Kim H; Hill, Robert D; Stasolla, Claudio

    2011-10-01

    Over the past few years non-symbiotic plant hemoglobins have been described in a variety of plant species where they fulfill several functions ranging from detoxification processes to basic aspects of plant growth and post-embryonic development. To date no information is available on the role of hemoglobins during in vitro morphogenesis. Shoot organogenesis was induced in Arabidopsis lines constitutively expressing class 1, 2 and 3 hemoglobins (GLB1, 2 and 3) and lines in which the respective genes were either downregulated by RNAi (GLB1) or knocked out (GLB2 and GLB3). The process was executed by culturing root explants on an initial auxin-rich callus induction medium (CIM) followed by a transfer onto a cytokinin-containing shoot induction medium (SIM). While the repression of GLB2 inhibited organogenesis the over-expression of GLB1 or GLB2 enhanced the number of shoots produced in culture, and altered the transcript levels of genes participating in cytokinin perception and signalling. The up-regulation of GLB1 or GLB2 activated CKI1 and AHK3, genes encoding cytokinin receptors and affected the transcript levels of cytokinin responsive regulators (ARRs). The expression of Type-A ARRs (ARR4, 5, 7, 15, and 16), feed-back repressors of the cytokinin pathway, was repressed in both hemoglobin over-expressors whereas that of several Type-B ARRs (ARR2, 12, and 13), transcription activators of cytokinin-responsive genes, was induced. Such changes enhanced the sensitivity of the root explants to cytokinin allowing the 35S::GLB1 and 35S::GLB2 lines to produce shoots at low cytokinin concentrations which did not promote organogenesis in the WT line. These results show that manipulation of hemoglobin can modify shoot organogenesis in Arabidopsis and possibly in those systems partially or completely unresponsive to applications of exogenous cytokinins. PMID:21741261

  19. Channel catfish hemoglobin genes: identification, phylogenetic and syntenic analysis, and specific induction in response to heat stress.

    Science.gov (United States)

    Feng, Jianbin; Liu, Shikai; Wang, Xiuli; Wang, Ruijia; Zhang, Jiaren; Jiang, Yanliang; Li, Chao; Kaltenboeck, Ludmilla; Li, Jiale; Liu, Zhanjiang

    2014-03-01

    Hemoglobins transport oxygen from gill to inner organs in fish, and this process is affected by temperature, one of the major environmental factors for fish. The hemoglobin gene clusters have been well studied in humans and several model fish species, but remain largely unknown in catfish. Here, eight α- and six β-hemoglobin genes were identified and characterized in channel catfish. Genomic synteny analysis showed that these hemoglobin genes were separated into two unlinked clusters, the MN cluster containing six α- and six β-hemoglobin genes, and the LA cluster consisting of two α-hemoglobin genes. Channel catfish hemoglobin genes were ubiquitously expressed in all the 10 tested tissues from healthy fish, but exhibited higher expression level in spleen, head kidney, and trunk kidney. In response to heat stress, hemoglobin genes, especially MN Hbα4, MN Hbα5, MN Hbα6, MN Hbβ4, MN Hbβ5, MN Hbβ6, LA Hbα1, and LA Hbα2, presumably the embryonic hemoglobin genes, were drastically up-regulated in the gill and head kidney of heat-tolerant fishes, but not in these tissues of the heat-intolerant fish, suggesting the importance of the embryonic hemoglobin genes in coping with the low oxygen conditions under heat stress.

  20. The correlation of elderly’s fasting blood glucose, glycosylated serum protein, glycosylated hemoglobin levels and colorectal adenoma%老年人空腹血糖、糖化血清蛋白、糖化血红蛋白水平与结直肠腺瘤的关系

    Institute of Scientific and Technical Information of China (English)

    张伟伟; 司一民; 邢欣; 乔延国; 张如意; 杨娜

    2014-01-01

    目的:研究老年人空腹血糖、糖化血清蛋白、糖化血红蛋白水平与结直肠腺瘤的关系。方法对2010年8月~2013年8月本院干部病房265例60岁以上老年患者行结肠镜检查,根据是否患有结直肠腺瘤分为研究组及对照组,两组患者均无结直肠腺癌、结直肠手术史。测定两组患者空腹血糖、糖化血清蛋白、糖化血红蛋白水平,并进行对比分析。结果腺瘤组糖化血清蛋白、糖化血红蛋白均高于对照组,腺瘤组糖化血清蛋白(194.02±50.83)μmmol/L,糖化血红蛋白(6.39±1.13)%;对照组糖化血清蛋白(181.61±41.21)μmmol/L,糖化血红蛋白(6.16±0.87)%,2组间比较差异均有统计学意义,P<0.05。结论糖化血清蛋白、糖化血红蛋白水平高的老年患者结直肠腺瘤的风险较高。%Objective To study correlation of elderly’s fasting blood glucose, glycosylated serum protein, glycosylated hemoglobin level and colorectal adenoma. Methods A retrospective analysis was performed on 265 over 60-year old patients who were hospitalized in between August 2010 and August 2013 in our hospital and underwent colonoscopy exam. They were divided into two groups, colorectal adenoma group and non-colorectal adenoma group (control group). Their fasting blood glucose, glycosylated serum protein, and glycosylated hemoglobin levels of the two groups were analyzed. Results The levels of glycosylated serum protein, glycosylated hemoglobin in patients with colorectal adenoma were much higer than the control group ,P<0.05. In adenoma group, it reflected an average glycosylated serum protein value of 194.02 ± 50.83μmmol/L, glycosylated hemoglobin value of(6.39 ± 1.13)%;and in the control group, it reflected an average glycosylated serum protein value of 181.61 ± 41.21μmmol/L, glycosylated hemoglobin value of(6.16 ± 0.87)%. Conclusion The findings of this study indicate a higher risk colorectal adenomas in old patients who have high

  1. Níveis séricos de hemoglobina em adolescentes segundo estágio de maturação sexual Hemoglobin serum levels in adolescents according to sexual maturation stage

    Directory of Open Access Journals (Sweden)

    Maria Fernanda Petroli Frutuoso

    2003-06-01

    Full Text Available A adolescência constitui etapa de risco para o desenvolvimento da anemia ferropriva, uma vez que ocorre aumento da necessidade de ferro decorrente do crescimento estatural e da maturação biológica. Estudaram-se 130 adolescentes, de ambos os sexos, para verificar os valores de hemoglobina sérica em diferentes fases de maturação sexual. Utilizou-se o método de fotometria para dosar a hemoglobina sérica e realizou-se auto-avaliação do estágio de maturação sexual com base nos critérios de Tanner. Os níveis médios de hemoglobina foram semelhantes entre sexos, bem como entre meninas que menstruavam ou não. O nível médio de hemoglobina foi de 13,3g/dL tanto para os meninos como para as meninas (p=0,64, com desvios-padrão de 1,12 e de 0,83, respectivamente. Entre os adolescentes estudados, 7,7% tinham anemia ferropriva. Recomenda-se atenção ao grupo de adolescentes, devido ao aumento da necessidade de ferro durante o estirão de crescimento, principalmente entre as meninas, aumentando a suscetibilidade à anemia.During adolescence, the risk of development of iron-deficiency anemia is higher because of the growth spurt and the sexual maturation which increase the iron requirement. One hundred and thirty adolescents (males and females were studied, in order to assess the serum hemoglobin values in different sexual maturation stages. The photometric method was used and a self-evaluation of the sexual maturation stage based on Tanner's criteria was applied. The average hemoglobin values were similar for both sexes, as well for girls who had menstruated or not. The average hemoglobin values were 13.3 (s.d. 1.12g/dL for males and 13.3 (s.d. 0.83g/dL for females (p=0.64. Among the studied adolescents, 7.7% had iron-deficiency anemia. Due to the iron requirement increase during the growth spurt, mainly in females, and the higher susceptibility to iron deficiency anemia, special attention to the adolescents is recommended.

  2. Purification of diverse hemoglobins by metal salt precipitation.

    Science.gov (United States)

    Zimmerman, Devon; Dienes, Jack; Abdulmalik, Osheiza; Elmer, Jacob J

    2016-09-01

    Although donated blood is the preferred material for transfusion, its limited availability and stringent storage requirements have motivated the development of blood substitutes. The giant extracellular hemoglobin (aka erythrocruorin) of the earthworm Lumbricus terrestris (LtEc) has shown promise as a blood substitute, but an efficient purification method for LtEc must be developed to meet the potential large demand for blood substitutes. In this work, an optimized purification process that uses divalent and trivalent metal salts to selectively precipitate human, earthworm, and bloodworm hemoglobin (HbA, LtEc, and GdHb, respectively) from crude solutions was developed. Although several metal ions were able to selectively precipitate LtEc, Zn(2+) and Ni(2+) provided the lowest heme oxidation and highest overall yield of LtEc. In contrast, Zn(2+) was the only metal ion that completely precipitated HbA and GdHb. Polyacrylamide gel electrophoresis (PAGE) analysis shows that metal precipitation removes several impurities to provide highly pure hemoglobin samples. Heme oxidation levels were relatively low for Zn(2+)-purified HbA and LtEc (2.4±1.3% and 5.3±2.1%, respectively), but slightly higher for Ni(2+)-purified LtEc (8.4±1.2%). The oxygen affinity and cooperativity of the precipitated samples are also identical to samples purified with tangential flow filtration (TFF) alone, indicating the metal precipitation does not significantly affect the function of the hemoglobins. Overall, these results show that hemoglobins from several different species can be highly purified using a combination of metal (Zn(2+)) precipitation and tangential flow filtration. PMID:26363116

  3. Clinical value analysis of glycosylated hemoglobin levels in type 2 diabetes combined with stroke elderly patients%糖化血红蛋白水平对老年2型糖尿病合并脑卒中患者的临床价值分析

    Institute of Scientific and Technical Information of China (English)

    王洪光

    2014-01-01

    目的:探讨糖化血红蛋白(HbA1c)水平对老年2型糖尿病合并脑卒中患者的临床价值。方法选择2012年6月至2013年6月本院收治的84例老年2型糖尿病合并脑卒中患者,根据HbA1c的检查水平分为观察组(HbA1c>6.5%)与对照组(HbA1c<6.5%),两组患者均给予4周常规治疗。比较两组患者脑卒中的复发情况及神经功能缺损评分。结果观察组患者的脑卒中复发率、治疗4周后以及出院3个月后神经功能缺损评分均明显高于对照组,差异具有显著性(P<0.05)。结论HbA1c>6.5%的老年2型糖尿病合并脑卒中患者脑卒中复发率和死亡率较高。%Objective To investigate clinical value analysis of glycosylated hemoglobin levels in type 2 diabetes combined with stroke elderly patients. Method Totally 84 cases of type 2 diabetes combined with stroke elderly patients from June 2012 to June 2013 were divided according to the level of glycated hemoglobin. The observation group was 40 patients with glycosylated hemoglobin>6.5%, and the control group was 44 patients with glycosylated hemoglobin 6.5%of type 2 diabetes combined with stroke elderly patients has more stroke recurrence and mortality.

  4. Changes in hemoglobin concentration during chemoradiation of locally advanced head and neck tumors

    International Nuclear Information System (INIS)

    Background: Despite multimodality treatment strategies of locally advanced head and neck cancers long-term results leave much to be desired. There is evidence that oxygenation status of head and neck tumors is directly influenced by the hemoglobin concentration. The aim of this study was to verify changes in the hemoglobin level during combined radio-chemotherapy of locally advanced head and neck tumors. Patients and methods: Sixty-eight patients with locally advanced head and neck cancer had primary or adjuvant radiotherapy with doses of 60 to 74 Gy in combination with cisplatin (±5-FU) or carboplatin chemotherapy in the first and fifth week of treatment. Hemoglobin levels were analyzed before and at the end of radiotherapy. Results: In 41% of all patients the initial hemoglobin concentration was below normal levels. The mean hemoglobin values in all patients dropped significantly from 12.9±1.7 g/dl before to 11.6±1.6 g/dl at the end of treatment. In 12 cases (18%) allogeneic erythrocytes had to be transfused during treatment. At the end of treatment 76% of all patients had anemic hemoglobin levels. In the groups of patients with cisplatin and carboplatin chemotherapy a significant decrease in hemoglobin levels was seen without meaningful statistical difference between these 2 groups. Conclusions: In patients with locally advanced head and neck cancer a high initial rate of anemia was registered (41%): This rate was nearly doubled during chemoradiation (76%). Since several studies have shown a correlation between hemoglobin levels and local tumor control, there is evidence, that this group might benefit from correcting anemia before combined radio-chemotherapy. (orig.)

  5. Interaction of Human Hemoglobin with Methotrexate

    Science.gov (United States)

    Zaharia, M.; Gradinaru, R.

    2015-05-01

    This study focuses on the interaction between methotrexate and human hemoglobin using steady-state ultraviolet-visible and fluorescence quenching methods. Fluorescence quenching was found to be valuable in assessing drug binding to hemoglobin. The quenching of methotrexate is slightly smaller than the quenching observed with related analogs (dihydrofolate and tetrahydrofolate). The quenching studies were performed at four different temperatures and various pH values. The number of binding sites for tryptophan is ~1. Parameter-dependent assays revealed that electrostatic forces play an essential role in the methotrexate-hemoglobin interaction. Furthermore, the complex was easily eluted using gel filtration chromatography.

  6. Hemoglobin, ferritin and thyroid profile in women with chronic telogen effluvium

    Directory of Open Access Journals (Sweden)

    Jayashankar C.A.

    2016-01-01

    Results: One hundred patients with a mean age of 29 years, of which 35% in the age group 18-25 years participated in the study. Prevalence of CTE was high (91% in the age group 18-40 years. Most of the women (71% presented with complaints of hair loss, within 12 months of occurrence. Hemoglobin levels were found to be significantly low in 66%, low serum ferritin in 76%, and hypothyroidism in 24% of patients. Out of the 66 women with altered blood hemoglobin levels, 72.7% has significant stress levels and 60.4% of them were working women. Ten patients had significantly low serum ferritin levels of < 40 and #956;g/L, in spite of having normal hemoglobin levels. Nine patients had low serum ferritin and low thyroid levels. As many as eight patients had lower than normal levels of all the three markers. Conclusions: A significant number of patients had low hemoglobin, serum ferritin and thyroid levels, confirming a strong association of anemic conditions with CTE. Therefore, hemoglobin level, serum ferritin and thyroid levels need to be determined in CTE patients. Stress also plays a significant role in the causation of hair loss in women. [Int J Res Med Sci 2016; 4(1.000: 152-155

  7. Stabilized hemoglobins as acellular resuscitative fluids.

    Science.gov (United States)

    Cerny, L C; Green, A; Noga, B; Cerny, E R

    1992-01-01

    This study reports some recent work dealing with the stabilization of the tetramers of hemoglobin. It is shown that by using a variety of diacids, it is possible to increase the P50 above that of stroma free hemoglobin. In order to lengthen the retention times in the circulatory system, the stabilized hemoglobins were complexed with both hydroxyethyl starch polymers and polyol tetronic polymers. The resulting hemoglobin-polymer compounds were then freeze-dried. It was possible to reconstitute the powder by the addition of physiological saline when needed. The methods presented here appear to be appear to be as effective as using pyridoxal phosphate but at a fraction of the cost. PMID:1391448

  8. 137Cs baseline levels in the Mediterranean and Black Sea: A cross-basin survey of the CIESM Mediterranean Mussel Watch programme

    International Nuclear Information System (INIS)

    The common mussel Mytilus galloprovincialis was selected as unique biomonitor species to implement a regional monitoring programme, the CIESM Mediterranean Mussel Watch (MMW), in the Mediterranean and Black Seas. As of today, and upon standardization of the methodological approach, the MMW Network has been able to quantify 137Cs levels in mussels from 60 coastal stations and to produce the first distribution map of this artificial radionuclide at the scale of the entire Mediterranean and Black Seas. While measured 137Cs levels were found to be very low (usually -1 wet wt) 137Cs activity concentrations in the Black Sea and North Aegean Sea were up to two orders of magnitude higher than those in the western Mediterranean Basin. Such effects, far from representing a threat to human populations or the environment, reflect a persistent signature of the Chernobyl fallout in this area

  9. {sup 137}Cs baseline levels in the Mediterranean and Black Sea: A cross-basin survey of the CIESM Mediterranean Mussel Watch programme

    Energy Technology Data Exchange (ETDEWEB)

    Thebault, Herve [IRSN, LERCM, Centre Ifremer, BP 330, F-83507 La Seyne sur Mer (France); Rodriguez y Baena, Alessia M. [International Commission for the Scientific Exploration of the Mediterranean Sea (CIESM), 16 Blvd de Suisse, MC-98000 Principality of Monaco (Monaco); Andral, Bruno [Ifremer, BP 330, F-83507 La Seyne sur Mer (France); Barisic, Delko [Center for Marine and Environmental Research, Lab. for Trace Physical Chemistry, Ruder Boskovic Inst., P.O. Box 1016, Bijenicka 54, 10002 Zagreb (Croatia); Albaladejo, Jose Benedicto [Inst. Espanol de Oceanografi' a (IEO), Centro Oceanografico de Murcia - Calle Varadero No. 1, 30740 San Pedro del Pinatar (Spain); Bologa, Alexandru S. [National Institute for Marine Research and Development ' Grigore Antipa' , RO-900581, Constantza (Romania); Boudjenoun, Redouane [Commissariat a l' Energie Atomique (COMENA), Centre de Radioprotection Recherche Nucleaire d' Algeret de Surete, Lab. d' Etudes d' Impact Radiologique, 2 Blvd F. Fanon - BP 399 Alger-Gare, 16000 Alger (Algeria); Delfanti, Roberta [Ente per le Nuove Tecnologie, l' Energia, e l' Ambiente (ENEA), Marine Environment Research Centre, La Spezia (Italy); Egorov, Victor N. [Inst. of Biology of the Southern Seas (IBSS), National Academy of Sciences of the Ukraine, 2 Porspekt Nakhimova, 99 011 Sevastopol, Crimea (Ukraine); El Khoukhi, Tahar [Centre d' Etudes Nucleaires de la Maamora (CNESTEN), BP 1382 RP Rabat 10001 (Morocco); Florou, Heleni [National Centre for Scientific Research ' Demokritos' , Aghia Paraskevi 153 10, P.O. Box 60228, Athens (Greece); Kniewald, Goran [Center for Marine and Environmental Research, Lab. for Trace Physical Chemistry, Ruder Boskovic Inst., P.O. Box 1016, Bijenicka 54, 10002 Zagreb (Croatia); Noureddine, Abdelkader [Commissariat a l' Energie Atomique (COMENA), Centre de Radioprotection Recherche Nucleaire d' Algeret de Surete, Lab. d' Etudes d' Impact Radiologique, 2 Blvd F. Fanon - BP 399 Alger-Gare, 16000 Alger (Algeria)] (and others)

    2008-07-01

    The common mussel Mytilus galloprovincialis was selected as unique biomonitor species to implement a regional monitoring programme, the CIESM Mediterranean Mussel Watch (MMW), in the Mediterranean and Black Seas. As of today, and upon standardization of the methodological approach, the MMW Network has been able to quantify {sup 137}Cs levels in mussels from 60 coastal stations and to produce the first distribution map of this artificial radionuclide at the scale of the entire Mediterranean and Black Seas. While measured {sup 137}Cs levels were found to be very low (usually <1 Bq kg{sup -1} wet wt) {sup 137}Cs activity concentrations in the Black Sea and North Aegean Sea were up to two orders of magnitude higher than those in the western Mediterranean Basin. Such effects, far from representing a threat to human populations or the environment, reflect a persistent signature of the Chernobyl fallout in this area.

  10. Double blind randomized placebo-controlled trial on the effects of testosterone supplementation in elderly men with moderate to low testosterone levels: design and baseline characteristics [ISRCTN23688581

    OpenAIRE

    Verhaar Harald JJ; Sukel-Helleman Marja; Emmelot-Vonk Marielle H; Nakhai Pour Hamid Reza; Grobbee Diederick E; van der Schouw Yvonne T

    2006-01-01

    Abstract In ageing men testosterone levels decline, while cognitive function, muscle and bone mass, sexual hair growth, libido and sexual activity decline and the risk of cardiovascular diseases increase. We set up a double-blind, randomized placebo-controlled trial to investigate the effects of testosterone supplementation on functional mobility, quality of life, body composition, cognitive function, vascular function and risk factors, and bone mineral density in older hypogonadal men. We re...

  11. Environmental Baseline File National Transportation

    International Nuclear Information System (INIS)

    This Environmental Baseline File summarizes and consolidates information related to the national-level transportation of commercial spent nuclear fuel. Topics addressed include: shipments of commercial spent nuclear fuel based on mostly truck and mostly rail shipping scenarios; transportation routing for commercial spent nuclear fuel sites and DOE sites; radionuclide inventories for various shipping container capacities; transportation routing; populations along transportation routes; urbanized area population densities; the impacts of historical, reasonably foreseeable, and general transportation; state-level food transfer factors; Federal Guidance Report No. 11 and 12 radionuclide dose conversion factors; and national average atmospheric conditions

  12. Low hemoglobin deferral in blood donors

    OpenAIRE

    Mast, Alan E.

    2013-01-01

    Low hemoglobin deferral occurs in about 10% of attempted whole blood donations and commonly is a consequence of iron deficiency anemia. Pre-menopausal women often have iron deficiency anemia caused by menstruation and pregnancy and have low hemoglobin deferral on their first donation attempt. Frequent donors also develop iron deficiency and iron deficiency anemia because blood donation removes a large amount of iron from the donor and the 56-day minimum inter-donation interval for donors in t...

  13. Influence of hemoglobin level during early gestation on the development of cognition of preschool children%母亲孕早期血红蛋白水平与学龄前儿童智力发育的关系

    Institute of Scientific and Technical Information of China (English)

    杨雷; 任爱国; 刘建蒙; 叶荣伟; 洪世欣; 郑俊池

    2010-01-01

    目的 探讨母亲孕早期血红蛋白(Hb)水平对学龄前儿童智力发育的影响.方法 研究对象为浙江、江苏省13市(县)在1993-1996年期间分娩的妇女,2000-2001年对这些妇女所生育的儿童(平均68月龄)随机抽样,共得到3609个母子对.使用中国-韦氏幼儿智力量表对儿童进行智力测试;母亲孕早期的Hb浓度在首次产前检查时获得.分析母亲孕早期Hb浓度与所生儿童全量表智商、语言智商和操作智商得分之间的关系.结果 孕早期贫血组妇女所生儿童的语言智商、操作智商以及全量表智商得分,比非贫血组妇女所生儿童的智商得分分别高0.6、0.9和0.8分.调整儿童性别、智力测量时月龄、地区、产次以及母亲智商、文化程度、职业等因素后,未发现母亲孕早期贫血与儿童低语言智商、操作智商和全量表智商的风险之间存在统计学联系.按照每20个百分位间隔将妊娠期妇女孕早期Hb分成5组分析,Hb浓度偏低组(Hb<103 g/L)、中等组(110 g/L≤Hb<116 g/L)、偏高组(Hb≥124g/L)妇女所生儿童的语言智商得分分别为91.6±18.9、92.8±18.2、90.3±18.6;操作智商得分分别为104.7±15.2、104.5±14.3、103.5±15.1,全量表智商得分分别为97.8±17.3、98.4±16.3、96.4±17.4.调整混杂因素后,孕早期Hb偏高组妇女所生儿童低语言智商及低全量表智商得分的风险分别比孕早期Hb中等浓度组妇女所生儿童高54%(OR=1.54,95%CI:1.13~2.11)和53%(OR=1.53,95%CI:1.10~2.12),但未发现与儿童低操作智商得分风险存在统计学关联.母亲孕早期低Hb水平与儿童低语言、操作或全量表智商风险之间不存在统计学联系.结论 母亲孕早期过高的Hb浓度可能对其所生子女的语言智商有不利影响.%Objective The purpose of this study was to evaluate the effect of hemoglobin (Hb) level during early gestation on the cognitive development of children at 4-6 years of age.Methods A

  14. Hemoglobin parameters from diffuse reflectance data

    Science.gov (United States)

    Mourant, Judith R.; Marina, Oana C.; Hebert, Tiffany M.; Kaur, Gurpreet; Smith, Harriet O.

    2014-01-01

    Abstract. Tissue vasculature is altered when cancer develops. Consequently, noninvasive methods of monitoring blood vessel size, density, and oxygenation would be valuable. Simple spectroscopy employing fiber optic probes to measure backscattering can potentially determine hemoglobin parameters. However, heterogeneity of blood distribution, the dependence of the tissue-volume-sampled on scattering and absorption, and the potential compression of tissue all hinder the accurate determination of hemoglobin parameters. We address each of these issues. A simple derivation of a correction factor for the absorption coefficient, μa, is presented. This correction factor depends not only on the vessel size, as others have shown, but also on the density of blood vessels. Monte Carlo simulations were used to determine the dependence of an effective pathlength of light through tissue which is parameterized as a ninth-order polynomial function of μa. The hemoglobin bands of backscattering spectra of cervical tissue are fit using these expressions to obtain effective blood vessel size and density, tissue hemoglobin concentration, and oxygenation. Hemoglobin concentration and vessel density were found to depend on the pressure applied during in vivo acquisition of the spectra. It is also shown that determined vessel size depends on the blood hemoglobin concentration used. PMID:24671524

  15. 21 CFR 864.7470 - Glycosylated hemoglobin assay.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Glycosylated hemoglobin assay. 864.7470 Section... Glycosylated hemoglobin assay. (a) Identification. A glycosylated hemoglobin assay is a device used to measure the glycosylated hemoglobins (A1a, A1b, and A1c) in a patient's blood by a column...

  16. Hemoglobin concentrations and associated factors in adolescentes from Recife, Brazil

    Directory of Open Access Journals (Sweden)

    Elisângela Barros Soares Mendonça

    2014-10-01

    Full Text Available OBJECTIVE: To estimate the prevalence of anemia and associated factors in adolescents from the city of Recife in Pernambuco state. METHODS: This is a cross-sectional study, involving a random sample of 256 adolescents of both genders, aged 13 to 18, whose hemoglobin concentrations were evaluated, along with their nutritional status and socioeconomic and demographic characteristics. RESULTS: The prevalence of inadequate hemoglobin concentrations was 10.2% [CI95%=6.7-14.5], reaching levels considered as mild anemia (9 g/dL 0.05, nor with socioeconomic or demographic characteristics (p>0.05. CONCLUSION: Although the prevalence of anemia was low and classified as a mild health problem, preventive nutrition education involving the dissemination of healthy eating habits in schools and encouraging the consumption of iron-rich foods are strongly recommended.

  17. Efetividade de diferentes estratégias de intervenção com ferro nos níveis de hemoglobina e ferritina em escolares de Teresina, Piauí, Brasil Effectiveness of different iron supplementation strategies on hemoglobin and ferritin levels among schoolchildren in Teresina, Piauí State, Brazil

    Directory of Open Access Journals (Sweden)

    Marize Melo dos Santos

    2007-07-01

    Full Text Available O estudo avaliou o efeito das intervenções com sulfato ferroso e com ferro bisglicina quelato nas concentrações de hemoglobina e ferritina sérica em escolares de 7-11 anos, de ambos os sexos, de Teresina, Piauí, Brasil. Foi desenvolvido ensaio clínico-comunitário, randomizado, envolvendo 138 escolares, com níveis de hemoglobina 0,05 na comparação intergrupos. Nenhum impacto foi observado (p > 0,05 nas reservas corporais de ferro. Entretanto, escolares que apresentaram depleção das reservas corporais de ferro ( 0,05 entre os grupos de tratamento. Os resultados confirmam a efetividade das intervenções e ratificam o uso do esquema semanal com sulfato ferroso e com ferro bisglicina quelato no tratamento da deficiência do mineral e da anemia ferropriva.This study evaluated the effectiveness of supplementation with ferrous sulfate and iron bis-glycinate chelate on hemoglobin and serum ferritin levels among schoolchildren (7-11 years of both sexes. A randomized community-based trial including 138 anemic children (hemoglobin 0.05. No effect was observed on body iron for either intervention (p > 0.05. Children with depleted iron stores ( 0.05 was observed. The results confirm the effectiveness of the iron supplementation interventions and corroborate the use of iron salts or ferrous bisglycinate chelate on a weekly basis to overcome iron deficiency and anemia.

  18. Level of polychlorinated biphenyls in the lower troposphere of the North-and South-Atlantic Ocean. Studies of global baseline pollution. Pt. 17

    International Nuclear Information System (INIS)

    Fourteen polychlorinated biphenyl (PCB) congeners were quantified in air samples of the tropospheric boundary layer of the Atlantic Ocean. The samples were taken on the German research vessel 'Polarstern' during north-south cruises across the Atlantic Ocean (1990, 1991), and on the Capo Verde islands in the North Atlantic Ocean (1992). Values for the sum of PCB were between 48 pg/m3 (values for the seven indicator congeners were [ in pg/m3]: PCB 28: 1.3; PCB 52: 5.0; PCB 101: 3.0; PCB 118: ≤0.5; PCB 138: 1; PCB 153: 1; PCB 180: 3 (values for the seven indicator congeners were [in pg/m3]: PCB 28: 2.3; PCB 52: 3.4; PCB 101: ≤0.5; PCB 118: 3 (values for the seven indicator congeners were [in pg/m3]: PCB 28: 2.6; PCB 52: 11.7; PCB 101: 28.4; PCB 118: 9; PCB 138: 21; PCB 153: 18; PCB 180: 5.5) were measured of the coast of South Patagonia. A difference depending on latitude and on terrestrial influenced air masses between the lower and the higher chlorinated congeners was observed. The levels of three- and tetrachlorinated congeners were highest in the Trade Wind regions. The contents of the higher chlorinated congeners had maxima in samples influenced by continental air masses. A correlation of the levels of the lower chlorinated congeners in air over the South Atlantic with the surface water temperature and thus with the temperature dependent gas/water partition coefficient Kgw was observed. (orig.)

  19. Baseline levels of bioaerosols and volatile organic compounds around a municipal waste incinerator prior to the construction of a mechanical-biological treatment plant

    International Nuclear Information System (INIS)

    New waste management programs are currently aimed at developing alternative treatment technologies such as mechanical-biological treatment (MBT) and composting plants. However, there is still a high uncertainty concerning the chemical and microbiological risks for human health, not only for workers of these facilities, but also for the population living in the neighborhood. A new MBT plant is planned to be constructed adjacently to a municipal solid waste incinerator (MSWI) in Tarragona (Catalonia, Spain). In order to evaluate its potential impact and to differentiate the impacts of MSWI from those of the MBT when the latter is operative, a pre-operational survey was initiated by determining the concentrations of 20 volatile organic compounds (VOCs) and bioaerosols (total bacteria, Gram-negative bacteria, fungi and Aspergillus fumigatus) in airborne samples around the MSWI. The results indicated that the current concentrations of bioaerosols (ranges: 382-3882, 18-790, 44-926, and 3 for fungi at 25 deg. C, fungi at 37 deg. C, total bacteria, and Gram-negative bacteria, respectively) and VOCs (ranging from 0.9 to 121.2 μg/m3) are very low in comparison to reported levels in indoor and outdoor air in composting and MBT plants, as well in urban and industrial zones. With the exception of total bacteria, no correlations were observed between the environmental concentrations of biological agents and the direction/distance from the facility. However, total bacteria presented significantly higher levels downwind. Moreover, a non-significant increase of VOCs was detected in sites closer to the incinerator, which means that the MSWI could have a very minor impact on the surrounding environment.

  20. The renal handling of hemoglobin. I. Glomerular filtration.

    Science.gov (United States)

    Bunn, H F; Esham, W T; Bull, R W

    1969-05-01

    The glomerular filtration of hemoglobin (alpha(2)beta(2)) was studied under conditions in which its dissociation into alphabeta dimers was experimentally altered. Rats receiving hemoglobin treated with the sulfhydryl reagent bis(N-maleimidomethyl) ether (BME) showed a much lower renal excretion and prolonged plasma survival as compared with animals injected with untreated hemoglobin. Plasma disappearance was also prolonged in dogs receiving BME hemoglobin. Gel filtration data indicated that under physiological conditions, BME hemoglobin had impaired subunit dissociation. In addition, BME hemoglobin showed a very high oxygen affinity and a decreased rate of auto-oxidation. Glomerular filtration was enhanced under conditions which favor the dissociation of hemoglobin into dimers. Cat hemoglobin, which forms subunits much more extensively than canine hemoglobin, was excreted more readily by the rat kidney. The renal uptake of (59)Fe hemoglobin injected intra-arterially into rabbits varied inversely with the concentration of the injected dose.

  1. Multimeric hemoglobin of the Australian brine shrimp Parartemia.

    Science.gov (United States)

    Coleman, M; Matthews, C M; Trotman, C N

    2001-04-01

    The hemoglobin molecule of the commercially important brine shrimp Artemia sp. has been used extensively as a model for the study of molecular evolution. It consists of nine globin domains joined by short linker sequences, and these domains are believed to have originated through a series of duplications from an original globin gene. In addition, in Artemia, two different polymers of hemoglobin, called C and T, are found which differ by 11.7% at the amino acid level and are believed to have diverged about 60 MYA. This provides a set of data of 18 globin domain sequences that have evolved in the same organism. The pattern of amino acid substitution between these two polymers is unusual, with pairs of equivalent domains displaying differences of up to 2.7-fold in total amino acid substitution. Such differences would reflect a similar range of molecular-clock rates in what appear to be duplicate, structurally equivalent domains. In order to provide a reference outgroup, we sequenced the cDNA for a nine-domain hemoglobin (P) from another genus of brine shrimp, Parartemia zietziana, which differs morphologically and ecologically from Artemia and is endemic to Australia. Parartemia produces only one hundredth the amount of hemoglobin that Artemia produces and does not upregulate production in response to low oxygen partial pressure. Comparison of the globin domains at the amino acid and DNA levels suggests that the Artemia globin T gene has accumulated substitutions differently from the Parartemia P and Artemia C globin genes. We discuss the questions of accelerated evolution after duplication and possible functions for the Parartemia globin. PMID:11264409

  2. Glycosylated hemoglobin and hyperbaric oxygen coverage denials.

    Science.gov (United States)

    Moffat, A D; Worth, E R; Weaver, L K

    2015-01-01

    Some Medicaid and Medicare fiscal intermediaries are denying hyperbaric oxygen (HBO2) therapy for diabetic foot ulcer (DFU) patients if the glycosylated hemoglobin (HbA1c) > 7.0%. We performed multiple PubMed searches for any diabetic wound healing clinical trial that documented HbA1c and had a wound healing endpoint. We scrutinized 30 peer-reviewed clinical trials, representing more than 4,400 patients. The average HbA1c from the intervention side of the studies was 8.6% (7.2% - 9.9%) and the control/sham side was 8.3% (6.0% - 10.6%). Twelve studies made a direct attempt to link HbA1c and wound healing. Four retrospective studies and one prospective cohort study assert that lower HbA1c favors wound healing, but review of the studies reveal design flaws that invalidate these conclusions. In total, 25 studies showed no direct correlation between HbA1c levels and wound healing. There was no randomized controlled trial (RCT) data demonstrating that HbA1c 7.0% is unfounded.

  3. Functional behavior of tortoise hemoglobin Geochelone denticulata.

    Science.gov (United States)

    Torsoni, M A; Stoppa, G R; Turra, A; Ogo, S H

    2002-11-01

    The hemolysate from Geochelone denticulata contains two main hemoglobin components, as shown by ion exchange chromatography and polyacrylamide gel electrophoresis (PAGE). Electrophoresis under dissociating conditions showed three types of globin chains. The apparent molecular mass, as determined by gel filtration on Sephadex G-200, was compatible with tetrameric Hb, which was unable to polymerize. The G. denticulata Hb has a P50 value of 9.56 mm Hg at pH 7.4. The Hb oxygenation appears to be under the control of organic phosphates and hydrogen ion since it is strongly affected by those species. In the presence ATP or IHP the P50 values increased to 29.51 mm Hg and 54.95 mm Hg, respectively, at pH 7.4. The n50 was generally lower than 1.5 in stripped Hb, suggesting a dissociation of tetramers. In the presence of organic phosphates n50 values increased to approximately 2.5. The Bohr effect was evident in oxygen equilibrium experiments. The hematocrit (32%) and Hb concentration (5.7 mM as heme) of G. denticulata blood were substantially larger than those of G. carbonaria, but the methemoglobin levels were similar in both species, approximately 1%. Thus, the oxygen capacity of blood appears to be higher in G. denticulata than in G. carbonaria, particularly considering the functional properties of their Hbs, which would guarantee the survival of animals. PMID:12659022

  4. Glycosylated hemoglobin and hyperbaric oxygen coverage denials.

    Science.gov (United States)

    Moffat, A D; Worth, E R; Weaver, L K

    2015-01-01

    Some Medicaid and Medicare fiscal intermediaries are denying hyperbaric oxygen (HBO2) therapy for diabetic foot ulcer (DFU) patients if the glycosylated hemoglobin (HbA1c) > 7.0%. We performed multiple PubMed searches for any diabetic wound healing clinical trial that documented HbA1c and had a wound healing endpoint. We scrutinized 30 peer-reviewed clinical trials, representing more than 4,400 patients. The average HbA1c from the intervention side of the studies was 8.6% (7.2% - 9.9%) and the control/sham side was 8.3% (6.0% - 10.6%). Twelve studies made a direct attempt to link HbA1c and wound healing. Four retrospective studies and one prospective cohort study assert that lower HbA1c favors wound healing, but review of the studies reveal design flaws that invalidate these conclusions. In total, 25 studies showed no direct correlation between HbA1c levels and wound healing. There was no randomized controlled trial (RCT) data demonstrating that HbA1c controlled by the American Diabetes Association (ADA). Frequently, patients lack optimal blood glucose control when they have a limb-threatening DFU. The evidence supports that denying hyperbaric oxygen to those with HbA1c > 7.0% is unfounded. PMID:26152104

  5. Baseline levels of bioaerosols and volatile organic compounds around a municipal waste incinerator prior to the construction of a mechanical-biological treatment plant.

    Science.gov (United States)

    Vilavert, Lolita; Nadal, Martí; Inza, Isabel; Figueras, María J; Domingo, José L

    2009-09-01

    New waste management programs are currently aimed at developing alternative treatment technologies such as mechanical-biological treatment (MBT) and composting plants. However, there is still a high uncertainty concerning the chemical and microbiological risks for human health, not only for workers of these facilities, but also for the population living in the neighborhood. A new MBT plant is planned to be constructed adjacently to a municipal solid waste incinerator (MSWI) in Tarragona (Catalonia, Spain). In order to evaluate its potential impact and to differentiate the impacts of MSWI from those of the MBT when the latter is operative, a pre-operational survey was initiated by determining the concentrations of 20 volatile organic compounds (VOCs) and bioaerosols (total bacteria, gram-negative bacteria, fungi and Aspergillus fumigatus) in airborne samples around the MSWI. The results indicated that the current concentrations of bioaerosols (ranges: 382-3882, 18-790, 44-926, and urban and industrial zones. With the exception of total bacteria, no correlations were observed between the environmental concentrations of biological agents and the direction/distance from the facility. However, total bacteria presented significantly higher levels downwind. Moreover, a non-significant increase of VOCs was detected in sites closer to the incinerator, which means that the MSWI could have a very minor impact on the surrounding environment. PMID:19346120

  6. Benthic composition of a healthy subtropical reef: baseline species-level cover, with an emphasis on algae, in the Northwestern Hawaiian Islands.

    Directory of Open Access Journals (Sweden)

    Peter S Vroom

    Full Text Available The Northwestern Hawaiian Islands (NWHI are considered to be among the most pristine coral reef ecosystems remaining on the planet. These reefs naturally contain a high percent cover of algal functional groups with relatively low coral abundance and exhibit thriving fish communities dominated by top predators. Despite their highly protected status, these reefs are at risk from both direct and indirect anthropogenic sources. This study provides the first comprehensive data on percent coverage of algae, coral, and non-coral invertebrates at the species level, and investigates spatial diversity patterns across the archipelago to document benthic communities before further environmental changes occur in response to global warming and ocean acidification. Monitoring studies show that non-calcified macroalgae cover a greater percentage of substrate than corals on many high latitude reef sites. Forereef habitats in atoll systems often contain high abundances of the green macroalga Microdictyon setchellianum and the brown macroalga Lobophora variegata, yet these organisms were uncommon in forereefs of non-atoll systems. Species of the brown macroalgal genera Padina, Sargassum, and Stypopodium and the red macroalgal genus Laurencia became increasingly common in the two northernmost atolls of the island chain but were uncommon components of more southerly islands. Conversely, the scleractinian coral Porites lobata was common on forereefs at southern islands but less common at northern islands. Currently accepted paradigms of what constitutes a "healthy" reef may not apply to the subtropical NWHI, and metrics used to gauge reef health (e.g., high coral cover need to be reevaluated.

  7. Recombinant human erythropoietin and hemoglobin concentration at operation and during the postoperative period

    DEFF Research Database (Denmark)

    Qvist, N; Boesby, S; Wolff, B;

    1999-01-01

    In a double-blind placebo-controlled study we investigated the effect of recombinant human erythropoietin (r-HuEPO), on the perioperative hemoglobin concentration and the use of blood transfusions in patients undergoing elective colorectal surgery with a preoperative hemoglobin level ... for 4 days before surgery. There were no differences between the two groups with regard to sex, height, weight, serum electrolytes, and liver function tests at study entry. The preentry hemoglobin concentration was similar in the two groups, with a median value of 7.9 (range 5.3-8.5) mmol....../L in the erythropoietin group and 7.6 (5.1-8.5) mmol/L in the placebo group. On the day of surgery the median hemoglobin concentration was 7.8 (5. 3-9.2) mmol/L in the erythropoietin group and 7.2 (4.6-8.5) mmol/L in the placebo group (p

  8. Baseline levels of CD8+ T cells against survivin and survivin-2B in the blood of lung cancer patients and cancer-free individuals.

    Science.gov (United States)

    Karanikas, Vaios; Soukou, Faye; Kalala, Fani; Kerenidi, Theodora; Grammoustianou, Evangelia S; Gourgoulianis, Konstantinos I; Germenis, Anastasios E

    2008-11-01

    Survivin and its variant survivin-2B have been considered as potential candidates for cancer immunotherapy. The magnitude however of spontaneously occurring CD8(+) T cells circulating precursor CTLs (pCTL), has never been evaluated. We set out to measure in 20 patients with lung carcinomas and 5 aged matched healthy male individuals (expressing HLA-A2 and/or -A24), the frequency of pCTLs specific for two naturally processed and presented peptides of survivin (LTLGEFLKL presented by HLA-A2) and survivin-2B (AYACNTSTL presented by HLA-A24) since these peptides are the only ones used in immunotherapeutic trials. The frequency of peptide-specific pCTLs was estimated using a sensitive method that combines HLA-multimer flow cytometric technology with a previous step of in vitro amplification under limiting dilution conditions. Anti-survivin or anti-survivin-2B specific CTL clones were not detected in 17 out of the 21 tested patients, and in none of the healthy individuals. In a number of peripheral blood mononuclear cell microcultures of the remaining 4 patients, diffuse clusters stained weakly by the HLA-multimers were observed which were not amplified after further stimulation and, therefore, they were finally considered as negative. The significance of the levels of spontaneously occurring CTL-responses against survivin and survivin-2B peptides, in cancer patients and cancer-free subjects, remains to be elucidated and it would be interesting to be considered in relation to the clinical efficacy of anti-cancer vaccination protocols. PMID:18789878

  9. Rationing with baselines

    DEFF Research Database (Denmark)

    Hougaard, Jens Leth; Moreno-Ternero, Juan D.; Østerdal, Lars Peter Raahave

    2013-01-01

    We introduce a new operator for general rationing problems in which, besides conflicting claims, individual baselines play an important role in the rationing process. The operator builds onto ideas of composition, which are not only frequent in rationing, but also in related problems...... such as bargaining, choice, and queuing. We characterize the operator and show how it preserves some standard axioms in the literature on rationing. We also relate it to recent contributions in such literature....

  10. Correlation of glycosylated hemoglobin with microalbuminuria to predict renal damage in diabetic patients

    Directory of Open Access Journals (Sweden)

    Dipsha Kriplani

    2015-08-01

    Full Text Available Background: Regular screening of levels of glycosylated hemoglobin and microalbuminuria, diabetic nephropathy can be prevented. The aim was to assess and compare the levels of glycosylated hemoglobin, microalbuminuria and serum creatinine in type 2 diabetic patients divided in groups of those on default antidiabetic treatment compared with those on regular antidiabetic treatment and to assess its correlation in type 2 of diabetic nephropathy. Methods: Two hundred diabetic patients above 40 years of age and 200 age matched control subjects with levels of glycosylated hemoglobin < 6.5% and on regular antiglycemic therapy were selected. Fasting plasma sugar was estimated by the glucose oxidase (GOD - glucose peroxidase (POD. Glycosylated hemoglobin and microalbuminuria level was measured by the immunoturbidimetric method and serum creatinine estimation was done by the Jaffe's kinetic method. p value was drawn using the student's paired t-test. Results: There is a strong correlation between the increase in the levels of glycosylated hemoglobin with the corresponding rise in the levels of microalbuminuria and serum creatinine. Conclusion: Periodic surveillance of the levels of microalbuminuria should be carried out in the type 2 diabetic patients to prevent further damage by early detection of diabetic nephropathy. [Int J Res Med Sci 2015; 3(8.000: 2014-2019

  11. The TDAQ Baseline Architecture

    CERN Multimedia

    Wickens, F J

    The Trigger-DAQ community is currently busy preparing material for the DAQ, HLT and DCS TDR. Over the last few weeks a very important step has been a series of meetings to complete agreement on the baseline architecture. An overview of the architecture indicating some of the main parameters is shown in figure 1. As reported at the ATLAS Plenary during the February ATLAS week, the main area where the baseline had not yet been agreed was around the Read-Out System (ROS) and details in the DataFlow. The agreed architecture has: Read-Out Links (ROLs) from the RODs using S-Link; Read-Out Buffers (ROB) sited near the RODs, mounted in a chassis - today assumed to be a PC, using PCI bus at least for configuration, control and monitoring. The baseline assumes data aggregation, in the ROB and/or at the output (which could either be over a bus or in the network). Optimization of the data aggregation will be made in the coming months, but the current model has each ROB card receiving input from 4 ROLs, and 3 such c...

  12. Efeito da duração da amamentação exclusiva e mista sobre os níveis de hemoglobina nos primeiros seis meses de vida: um estudo de seguimento Effect of length of exclusive breastfeeding and mixed feeding on hemoglobin levels in the first six months of life: a follow-up study

    Directory of Open Access Journals (Sweden)

    Andréa Silva de Oliveira

    2010-02-01

    Full Text Available O objetivo deste estudo foi avaliar o efeito da duração da amamentação exclusiva e mista sobre os níveis de hemoglobina de lactentes. Trata-se de um estudo de seguimento com seis meses de duração, envolvendo 150 crianças nascidas na maternidade de Mutuípe, Bahia, Brasil. Mensalmente, as crianças eram submetidas ao exame de sangue para avaliação dos níveis de hemoglobina e realizava-se coleta de dados de consumo alimentar. Foram obtidos, ainda, dados obstétricos e sócio-econômicos. Utilizou-se o modelo linear de efeitos mistos para investigar as associações de interesse. Ao final do seguimento, a perda amostral foi de 15,3%, e detectou-se uma prevalência de 71,7% de anemia. Constatou-se um incremento de 0,091g/dL nos níveis de hemoglobina a cada mês de duração de aleitamento materno exclusivo (p = 0,031, enquanto, a cada mês de duração de aleitamento materno misto, os níveis de hemoglobina declinaram em 0,097g/dL (p = 0,017. Concluiu-se que as práticas alimentares inadequadas parecem estar diretamente envolvidas na etiologia da deficiência nutricional de ferro nesse período da vida.This six-month follow-up study aimed to assessing the effect of length of both exclusive breastfeeding and mixed feeding on hemoglobin levels in 150 infants born at the Mutuípe maternity facility in Bahia State, Brazil. Blood tests were performed monthly to assess the hemoglobin levels, at which time data were gathered on food intake. Obstetric and socioeconomic data were also collected. The linear mixed effects model was used to investigate the target associations. Loss to follow-up was 15.3%, and anemia prevalence was 71.7%. An increase of 0.091g/dL (p = 0.031 in hemoglobin levels was found for each month of exclusive breastfeeding, while a decrease of 0.097g/dL (p = 0.017 was shown for each month of mixed feeding. In conclusion, inadequate food practices appear to be directly involved in the etiology of iron deficiency anemia during

  13. IS HEMOGLOBIN E GENE WIDELY SPREAD IN THE STATE OF MADHYA PRADESH IN CENTRAL INDIA? EVIDENCE FROM FIVE TYPICAL FAMILIES

    Directory of Open Access Journals (Sweden)

    R S Balgir

    2014-09-01

    Full Text Available Background: Red cell inherited hemoglobin anomalies are commonly encountered in the central region of India. These cause a public health concern due to high degree of morbidity, mortality, and fetal loss in the backward, underprivileged, and vulnerable people. Purpose: To report five typical families of hemoglobin E disorders identified for the first time in the state of Madhya Pradesh from central India. Methods: Out of a total of 445 couples/families (excluding the present study with 1526 persons (848 males and 678 females referred from a tertiary hospital in central India for investigations of anemia/hemoglobinopathies during the period from March 2010 to February 2014, we came across five typical rare couples/families of hemoglobin E disorders worthy of detailed investigations. Laboratory investigations were carried out following the standard procedures after cross checking for quality control from time to time. Results: For the first time, we have encountered nine cases of heterozygous hemoglobin E trait, two members with hemoglobin E-β-thalassemia (double heterozygosity, two cases of sickle cell-hemoglobin E disease (double heterozygosity, and none with homozygous hemoglobin E. Cases  of hemoglobin E trait, hemoglobin E-β-thalassemia, sickle cell-β-thalassemia and sickle cell-E disease showed moderate to severe anemia, and target cells, and reduced values of red cell indices like RBC, Hb level, HCT, MCV, MCH and MCHC, representing abnormal hematological profile and clinical manifestations before blood transfusion. Conclusions: Double heterozygosity for hemoglobinopathies such as occurrence of β-thalassemia mutation with structurally abnormal hemoglobins (Hb S and Hb E is a rare entity, but occurs with severe clinical manifestations only in those areas or communities where these are highly prevalent, testifying the migrations and genetic admixture. Distribution of hemoglobin E and β-thalassemia in different districts of Madhya Pradesh

  14. Percutaneous Mitral Valve Repair in Mitral Regurgitation Reduces Cell-Free Hemoglobin and Improves Endothelial Function.

    Directory of Open Access Journals (Sweden)

    Christos Rammos

    Full Text Available Endothelial dysfunction is predictive for cardiovascular events and may be caused by decreased bioavailability of nitric oxide (NO. NO is scavenged by cell-free hemoglobin with reduction of bioavailable NO up to 70% subsequently deteriorating vascular function. While patients with mitral regurgitation (MR suffer from an impaired prognosis, mechanisms relating to coexistent vascular dysfunctions have not been described yet. Therapy of MR using a percutaneous mitral valve repair (PMVR approach has been shown to lead to significant clinical benefits. We here sought to investigate the role of endothelial function in MR and the potential impact of PMVR.Twenty-seven patients with moderate-to-severe MR treated with the MitraClip® device were enrolled in an open-label single-center observational study. Patients underwent clinical assessment, conventional echocardiography, and determination of endothelial function by measuring flow-mediated dilation (FMD of the brachial artery using high-resolution ultrasound at baseline and at 3-month follow-up. Patients with MR demonstrated decompartmentalized hemoglobin and reduced endothelial function (cell-free plasma hemoglobin in heme 28.9±3.8 μM, FMD 3.9±0.9%. Three months post-procedure, PMVR improved ejection fraction (from 41±3% to 46±3%, p = 0.03 and NYHA functional class (from 3.0±0.1 to 1.9±1.7, p<0.001. PMVR was associated with a decrease in cell free plasma hemoglobin (22.3±2.4 μM, p = 0.02 and improved endothelial functions (FMD 4.8±1.0%, p<0.0001.We demonstrate here that plasma from patients with MR contains significant amounts of cell-free hemoglobin, which is accompanied by endothelial dysfunction. PMVR therapy is associated with an improved hemoglobin decompartmentalization and vascular function.

  15. Extraction of Phospholipids from Human Erythrocyte Membranes by Hemoglobin Oxidation Products.

    Science.gov (United States)

    Brunauer, Linda S; Chen, James Y; Koontz, M Zachary; Davis, Kathryn K; O'Brien, Laura E; Wright, Emily M; Huestis, Wray H

    2016-06-01

    This investigation examines oxidation conditions under which hemoglobin extracts membrane phospholipid from erythrocytes and model membranes. In erythrocytes made echinocytic with exogenous phospholipid, addition of hemoglobin oxidized with hydrogen peroxide (H2O2) or Vitamin C (conditions that result in the formation of significant quantities of choleglobin), but not ferricyanide (which produces predominantly methemoglobin), induced dose-dependent shape reversion to less echinocytic forms, consistent with extraction of phospholipids from the exofacial side of the membrane. Erythrocytes preloaded with radiolabeled phosphatidylcholine or NBD-labeled phosphatidylcholine, phosphatidylglycerol or phosphatidic acid, exhibited greatest extraction of radiolabel or fluorescence signal with exogenous hemoglobin oxidized via H2O2 or Vitamin C, but not ferricyanide. However, with NBD-phosphatidylserine (a preferential inner monolayer intercalator), significantly less extraction of labeled lipid occurred with oxidized hemoglobin prepared under all three oxidizing conditions. In dimyristoylphosphatidylcholine liposomes containing radiolabeled phosphatidylcholine, phosphatidylserine or phosphatidylethanolamine, subsequent addition of hemoglobin oxidized with H2O2 or Vitamin C extracted radiolabeled lipid with significantly greater efficiency than ferricyanide-treated hemoglobin, with enhanced extraction detectable at levels approaching physiological plasma oxidant concentrations. Radiolabeled lipid extraction was comparable for phospholipids containing saturated acyl chains between 12 and 18 carbons but diminished significantly for oleoyl-containing phospholipids. Hemoglobin dimerization occurred at very low levels with H2O2 treatment, and even lower levels with Vitamin C treatment, and thus did not correlate to the high efficiency and consistent levels of lipid extraction observed with these treatments. These findings indicate that choleglobin extracts lipids from cell

  16. Baseline blood pressure, low- and high-density lipoproteins, and triglycerides and the risk of vascular events in the Stroke Prevention by Aggressive Reduction in Cholesterol Levels (SPARCL) trial

    DEFF Research Database (Denmark)

    Amarenco, Pierre; Goldstein, Larry B; Callahan, Alfred;

    2009-01-01

    cholesterol (LDL-C) and high-density lipoprotein cholesterol (HDL-C), and triglyceride levels. After 4.9 years of follow-up, there were 575 primary end points (fatal and nonfatal stroke), including 491 ischemic strokes, and 740 MCVEs (stroke plus myocardial infarction and vascular death). Cox regression...... AND RESULTS: The SPARCL trial randomized 4731 patients with recent stroke or transient ischemic attack (TIA) and no known coronary heart disease and LDL-C between 100 and 190 mg/dL to either atorvastatin 80 mg/d or placebo. Baseline assessment included SBP, DBP and measurements of low-density lipoprotein...... models analysis showed a trend (P>0.05 and PLDL/HDL ratio were each associated with MCVEs...

  17. 某医院50岁以上员工糖化血红蛋白水平调查%Analysis of Glycosylated Hemoglobin Levels of Employees at 50 Years Old in a Hospital

    Institute of Scientific and Technical Information of China (English)

    唐亮; 钱新瑜; 上官志敏

    2015-01-01

    Objective To investigate the distribution of glycosylated hemoglobin A1c (HbA1c)in staf aged over 50 years of a hospital.Methods The measured values of HbA1c and Hb in employees of 50 years old or more of a hospital were statisticed,and the distribution characteristics of the HbA1c were analyzed.With the inclusion criteria of Hb>110g/L and HbA1c110g/L且HbA1c<6.5%为纳入标准,分性别和年龄段分析其HbA1c与年龄之间的关系,建立二者之间的回归方程。结果共统计695例样本,按照标准纳入男性211例,女性406例,HbA1c值分别是(5.528±0.3624)和(5.537±0.3691),两者无统计学意义。随着年龄增长,HbA1c值逐渐增高,但50~60岁组HbA1c水平明显低于其他年龄组。 HbA1c与年龄的回归方程是:HbA1c%=5.024+0.008×年龄。按照HbA1c水平分组统计,HbA1c≥6.5%的占9.3%,HbA1c在5.7%~6.4%的“糖尿病高危人群”占23.6%。结论该医院50岁以上员工糖尿病患病率高,与全国水平相近,提示要加强健康教育,降低糖尿病的危害。

  18. Kericho CLinic-based ART Diagnostic Evaluation (CLADE: design, accrual, and baseline characteristics of a randomized controlled trial conducted in predominately rural, district-level, HIV clinics of Kenya.

    Directory of Open Access Journals (Sweden)

    Fredrick K Sawe

    Full Text Available Prospective clinical trial data regarding routine HIV-1 viral load (VL monitoring of antiretroviral therapy (ART in non-research clinics of Sub-Saharan Africa are needed for policy makers.CLinic-based ART Diagnostic Evaluation (CLADE is a randomized, controlled trial (RCT evaluating feasibility, superiority, and cost-effectiveness of routine VL vs. standard of care (clinical and immunological monitoring in adults initiating dual nucleoside reverse transcriptase inhibitor (NRTI+non-NRTI ART. Participants were randomized (1:1 at 7 predominately rural, non-research, district-level clinics of western Kenya. Descriptive statistics present accrual patterns and baseline cohort characteristics.Over 15 months, 820 adults enrolled at 7 sites with 86-152 enrolled per site. Monthly site enrollment ranged from 2-92 participants. Full (100% informed consent compliance was independently documented. Half (49.9% had HIV diagnosed through voluntary counseling and testing. Study arms were similar: mostly females (57.6% aged 37.6 (SD = 9.0 years with low CD4 (166 [SD = 106] cells/m3. Notable proportions had WHO Stage III or IV disease (28.7%, BMI <18.5 kg/m2 (23.1%, and a history of tuberculosis (5.6% or were receiving tuberculosis treatment (8.2% at ART initiation. In the routine VL arm, 407/409 (99.5% received baseline VL (234,577 SD = 151,055 copies/ml. All participants received lamivudine; 49.8% started zidovudine followed by 38.4% stavudine and 11.8% tenofovir; and, 64.4% received nevirapine as nNRTI (35.6% efavirenz.A RCT can be enrolled successfully in rural, non-research, resource limited, district-level clinics in western Kenya. Many adults presenting for ART have advanced HIV/AIDS, emphasizing the importance of universal HIV testing and linkage-to-care campaigns.ClinicalTrials.gov NCT01791556.

  19. Nitrosyl hemoglobins: EPR above 80 K

    Energy Technology Data Exchange (ETDEWEB)

    Wajnberg, E.; Bemski, G.; El-Jaick, L.J.; Alves, O.C.

    1995-03-01

    The EPR spectra of nitrosyl hemoglobin and myoglobin in different conditions (native, denatured and lyophilized), as well as of hematin-NO were obtained in the temperature range of 80 K-280 K. There is a substantial and reversible.decrease of the areas of the EPR spectra of all the hemoglobin samples above 150 K. The interpretation of the results implies the existence of two conformational states in thermal equilibrium only one of which is EPR detectable. Thermodynamical parameters are determined for the hexa and penta-coordinated cases. (author). 25 refs, 3 figs.

  20. Hemoglobin concentrations in waders vary with their strategies of migration: a comparative analysis.

    Science.gov (United States)

    Minias, Piotr; Kaczmarek, Krzysztof; Włodarczyk, Radosław; Janiszewski, Tomasz

    2013-05-01

    The aim of this study was to determine whether blood oxygen capacity of waders varies with respect to migration at both inter-specific and individual level. To verify this hypothesis we measured hemoglobin concentration in 875 waders from 14 species during their autumn migration through central Poland. In most of the species we found an increase in the hemoglobin levels along with increasing fat loads during the stopover period, which suggests that individual birds are able to elevate their oxygen-carrying capacity of blood prior to departure on a migratory flight. Positive relationship between hemoglobin concentrations of waders and their fat loads was confirmed at the inter-specific level by the comparative analysis of independent contrasts. Comparative analysis also demonstrated that hemoglobin concentrations were positively related with theoretical flight range and mean refueling rate during stopovers. The results indicate that species traveling according to the strategy of energy-minimization (short-distance migrants, low fat reserves, low refueling rates) have lower blood oxygen capacity in comparison to time-selected species (long-distance migrants, high fat reserves, high refueling rates). It remains uncertain whether high hemoglobin levels in long-distance migrants are a fixed evolutionary trait or a temporal physiological adaptation associated with carrying considerable fat load. PMID:23425637

  1. Association of glycosylated hemoglobin with the gene encoding CDKAL1 in the Korean Association Resource (KARE) study.

    Science.gov (United States)

    Ryu, Jihye; Lee, Chaeyoung

    2012-04-01

    Genome-wide associations with glycosylated hemoglobin, which reflects the long-term glycemia, were examined using two independent cohorts of the Korea Association Resource (KARE) consortium. We first identified sequence variants within a linkage disequilibrium block (r(2) > 0.98) in the intron 5 of cyclin-dependent kinase 5 regulatory subunit-associated protein 1-like 1 (CDKAL1) gene using 4,275 normoglycemic subjects of Cohort 1 (P glycosylated hemoglobin by affecting 1 h glucose level. Ultimately, accumulated effects on the glycosylated hemoglobin level by the genetic variation of CDKAL1 might affect susceptibility to type 2 diabetes mellitus.

  2. Mass Spectra and Ion Collision Cross Sections of Hemoglobin

    Science.gov (United States)

    Kang, Yang; Terrier, Peran; Douglas, D. J.

    2011-02-01

    Mass spectra of commercially obtained hemoglobin (Hb) show higher levels of monomer and dimer ions, heme-deficient dimer ions, and apo-monomer ions than hemoglobin freshly prepared from blood. This has previously been attributed to oxidation of commercial Hb. Further, it has been reported that that dimer ions from commercial bovine Hb have lower collision cross sections than low charge state monomer ions. To investigate these effects further, we have recorded mass spectra of fresh human Hb, commercial human and bovine Hb, fresh human Hb oxidized with H2O2, lyophilized fresh human Hb, fresh human Hb both lyophilized and chemically oxidized, and commercial human Hb oxidized with H2O2. Masses of α-monomer ions of all hemoglobins agree with the masses expected from the sequences within 3 Da or better. Mass spectra of the β chains of commercial Hb and oxidized fresh human Hb show a peak or shoulder on the high mass side, consistent with oxidation of the protein. Both commercial proteins and oxidized fresh human Hb produce heme-deficient dimers with masses 32 Da greater than expected and higher levels of monomer and dimer ions than fresh Hb. Lyophilization or oxidation of Hb both produce higher levels of monomer and dimer ions in mass spectra. Fresh human Hb, commercial human Hb, commercial bovine Hb, and oxidized commercial human Hb all give dimer ions with cross sections greater than monomer ions. Thus, neither oxidation of Hb or the difference in sequence between human and bovine Hb make substantial differences to cross sections of ions.

  3. Hemoglobin: A Nitric-Oxide Dioxygenase

    Directory of Open Access Journals (Sweden)

    Paul R. Gardner

    2012-01-01

    Full Text Available Members of the hemoglobin superfamily efficiently catalyze nitric-oxide dioxygenation, and when paired with native electron donors, function as NO dioxygenases (NODs. Indeed, the NOD function has emerged as a more common and ancient function than the well-known role in O2 transport-storage. Novel hemoglobins possessing a NOD function continue to be discovered in diverse life forms. Unique hemoglobin structures evolved, in part, for catalysis with different electron donors. The mechanism of NOD catalysis by representative single domain hemoglobins and multidomain flavohemoglobin occurs through a multistep mechanism involving O2 migration to the heme pocket, O2 binding-reduction, NO migration, radical-radical coupling, O-atom rearrangement, nitrate release, and heme iron re-reduction. Unraveling the physiological functions of multiple NODs with varying expression in organisms and the complexity of NO as both a poison and signaling molecule remain grand challenges for the NO field. NOD knockout organisms and cells expressing recombinant NODs are helping to advance our understanding of NO actions in microbial infection, plant senescence, cancer, mitochondrial function, iron metabolism, and tissue O2 homeostasis. NOD inhibitors are being pursued for therapeutic applications as antibiotics and antitumor agents. Transgenic NOD-expressing plants, fish, algae, and microbes are being developed for agriculture, aquaculture, and industry.

  4. Constraints on mutational pathways of hemoglobin evolution

    DEFF Research Database (Denmark)

    Kumar, Amit; Natarajan, Chandrasekhar; Moriyama, Hideaki;

    2016-01-01

    amino acid substitutions that occurred during an evolutionary reduction in hemoglobin (Hb)-O2 affinity in nightjars (nocturnal birds in the family Caprimulgidae).We selected nightjar Hbs for experimental study because ancestral sequence reconstructions indicated that the evolved reduction in Hb-O2...

  5. Unrecognized hemoglobin SE disease as microcytosis

    OpenAIRE

    Smith, Avery; Cooper, Barry; Guileyardo, Joseph; Mora, Adan

    2016-01-01

    Hemoglobin SE disease was first described during the 1950s as a relatively benign microcytosis, but increasing prevalence has revealed a predisposition towards vasoocclusive sickling. Recognition of SE hemoglobinopathies’ potential complications is crucial so medical measures can be utilized to avoid multiorgan injury.

  6. Impacto do uso de cereal adicionado de ferro sobre os níveis de hemoglobina e a antropometria de pré-escolares Effect of the use of iron-enriched cereal on the serum hemoglobin levels and anthropometric values of preschool children

    Directory of Open Access Journals (Sweden)

    Márcia Regina Vítolo

    1998-12-01

    Full Text Available A anemia na infância tem sido considerada um dos grandes desafios nutricionais nos países em desenvolvimento, seguida da desnutrição energética. Têm-se discutido muito a eficácia dos alimentos enriquecidos no combate a esse problema. O objetivo desse trabalho foi avaliar a aceitação diária de cereal adicionado de ferro e o seu impacto nos parâmetros antropométricos e nos níveis de hemoglobina de crianças de 1 a 4 anos de idade. Foram estudadas 54 crianças de uma creche municipal, de duas salas, sendo o grupo I constituído de 24 crianças com média de idade 2 anos e 2 meses e o grupo II constituído de 30 crianças de 3 anos e 6 meses. Essas crianças foram avaliadas, no início e no final da intervenção, quanto à ingestão alimentar, peso, estatura e níveis de hemoglobina sérica. Durante o período de dois meses, o café da manhã e o lanche da tarde, que eram constituídos de leite e pão, foram substituídos pelo cereal adicionado de ferro na forma de mingau, o que proporcionou aumento de 2 a 3mg de ferro por dia. Os resultados mostraram que houve aumento significante na adequação do índice P/E das crianças com desnutrição atual. Quanto aos níveis de hemoglobina sérica, o grupo I, cujo valor médio inicial foi de 9,9 ± 2,0g/dl, apresentou valor médio significantemente mais alto ao final do estudo, 11,4 ± 1,0g/dl (pAnemia and energetic malnutrition are considered to be the major nutritional challenges in developing countries. The efficacy of the various programs implemented to solve these problems has been assessed by studies with enriched food products. The objective of the present study was to evaluate the daily acceptance of an iron-enriched cereal, as well as its effect on the anthropometric parameters and hemoglobin levels of children aged 1 to 4 years old. Fifty-four children enrolled at a public day care unit participated in the study. They were divided into two groups: Group I with 24 children (median

  7. Baseline report for infiltration experiment

    International Nuclear Information System (INIS)

    reactive transport calculations and flow simulations were performed. The main objective of the predictive reactive transport calculations was to successfully integrate hydrodynamic processes and chemical reactions. The calculations were carried out using the TOUGHREACT code and with the EQ3/6-database implemented in the code. A detailed surface hydrological model version was also developed in order to study water balance components at the experiment area site and to evaluate the effects of the pumping of OL-KR14 on groundwater level in overburden soils and in shallow bedrock drillholes. This Report describes the objectives of the Infiltration Experiment, the experimental site and setup, and the results of the aforesaid baseline field investigations. In addition, an updated hydrogeological model of the area of interest as well as the results of predictive flow simulations and reactive transport calculations are presented. (orig.)

  8. Alpha chain hemoglobins with electrophoretic mobility similar to that of hemoglobin S in a newborn screening program

    Directory of Open Access Journals (Sweden)

    Marcilene Rezende Silva

    2013-01-01

    Full Text Available OBJECTIVE: To characterize alpha-chain variant hemoglobins with electric mobility similar to that of hemoglobin S in a newborn screening program. METHODS: βS allele and alpha-thalassemia deletions were investigated in 14 children who had undefined hemoglobin at birth and an electrophoretic profile similar to that of hemoglobin S when they were six months old. Gene sequencing and restriction enzymes (DdeI, BsaJI, NlaIV, Bsu36I and TaqI were used to identify hemoglobins. Clinical and hematological data were obtained from children who attended scheduled medical visits. RESULTS: The following alpha chain variants were found: seven children with hemoglobin Hasharon [alpha2 47(CE5 Asp>His, HbA2:c.142G>C], all associated with alpha-thalassemia, five with hemoglobin Ottawa [alpha1 15(A13 Gly>Arg, HBA1:c.46G>C], one with hemoglobin St Luke's [alpha1 95(G2 Pro>Arg, HBA1:c.287C>G] and another one with hemoglobin Etobicoke [alpha212 84(F5 Ser>Arg, HBA212:c.255C>G]. Two associations with hemoglobin S were found: one with hemoglobin Ottawa and one with hemoglobin St Luke's. The mutation underlying hemoglobin Etobicoke was located in a hybrid α212 allele in one child. There was no evidence of clinically relevant hemoglobins detected in this study. CONCLUSION: Apparently these are the first cases of hemoglobin Ottawa, St Luke's, Etobicoke and the α212 gene described in Brazil. The hemoglobins detected in this study may lead to false diagnosis of sickle cell trait or sickle cell disease when only isoelectric focusing is used in neonatal screening. Additional tests are necessary for the correct identification of hemoglobin variants.

  9. A new hemoglobin gene from soybean: a role for hemoglobin in all plants

    DEFF Research Database (Denmark)

    Anderson, C R; Jensen, E O; LLewellyn, D J;

    1996-01-01

    We have isolated a new hemoglobin gene from soybean. It is expressed in cotyledons, stems of seedlings, roots, young leaves, and in some cells in the nodules that are associated with the nitrogen-fixing Bradyrhizobium symbiont. This contrasts with the expression of the leghemoglobins, which...... are active only in the infected cells of the nodules. The deduced protein sequence of the new gene shows only 58% similarity to one of the soybean leghemoglobins, but 85-87% similarity to hemoglobins from the nonlegumes Parasponia, Casuarina, and barley. The pattern of expression and the gene sequence...... indicate that this new gene is a nonsymbiotic legume hemoglobin. The finding of this gene in legumes and similar genes in other species strengthens our previous suggestion that genomes of all plants contain hemoglobin genes. The specialized leghemoglobin gene family may have arisen from a preexisting...

  10. Efeitos do clampeamento tardio do cordão umbilical sobre os níveis de hemoglobina e ferritina em lactentes aos três meses de vida Effects of delayed cord clamping on hemoglobin and ferritin levels in infants at three months of age

    Directory of Open Access Journals (Sweden)

    Sonia Isoyama Venâncio

    2008-01-01

    Full Text Available Este estudo verificou o efeito do clampeamento tardio (um minuto após o nascimento do cordão umbilical sobre os níveis de hemoglobina e ferritina em crianças aos três meses de vida. Foram alocadas 325 mães e crianças, nascidas a termo, de parto vaginal e sem patologias (164 no grupo do clampeamento imediato e 161 no tardio em um hospital do Município de São Paulo, Brasil, em 2006. Realizaram-se dosagens de hemoglobina da mãe no pré-parto e de hemoglobina e ferritina do cordão umbilical. As crianças (69% foram acompanhadas no ambulatório, após três meses, ocorrendo coleta de sangue venoso para dosagem de hemoglobina e ferritina. Variáveis sócio-econômicas, reprodutivas, antropométricas e de alimentação da criança foram estudadas. Utilizou-se a regressão linear múltipla para a análise dos dados. O efeito do clampeamento tardio do cordão umbilical foi significativo somente para os níveis de ferritina das crianças aos três meses de idade (p = 0,040, sendo superior em 23,29ng/mL quando comparado às crianças submetidas ao clampeamento imediato. O procedimento pode se constituir em uma estratégia para prevenir a deficiência de ferro em lactentes.This study assessed the effect of delayed (1 minute after delivery clamping of the umbilical cord on hemoglobin and ferritin levels in infants at three months of age. Mothers and their infants born through vaginal delivery, at term, and without congenital anomalies (325 pairs were recruited at a hospital in São Paulo, Brazil, in 2006 (164 in the delayed clamping subgroup and 161 in the early clamping subgroup. Maternal hemoglobin at delivery, umbilical cord hemoglobin, and ferritin were recorded. At three months follow-up, venous blood samples were drawn from 224 (69% infants for hemoglobin and ferritin measurement. Socioeconomic, maternal reproductive, anthropometric, and infant feeding variables were studied. Multiple linear regression models were used to analyze the data

  11. Effects of Hemoglobin Variants on Hemoglobin A1c Values Measured Using a High-Performance Liquid Chromatography Method

    Science.gov (United States)

    De-La-Iglesia, Silvia; Ropero, Paloma; Nogueira-Salgueiro, Patricia; Santana-Benitez, Jesus

    2014-01-01

    Hemoglobin A1c (HbA1c) is routinely used to monitor long-term glycemic control and for diagnosing diabetes mellitus. However, hemoglobin (Hb) gene variants/modifications can affect the accuracy of some methods. The potential effect of Hb variants on HbA1c measurements was investigated using a high-performance liquid chromatography (HPLC) method compared with an immunoturbimetric assay. Fasting plasma glucose (FPG) and HbA1c levels were measured in 42 371 blood samples. Samples producing abnormal chromatograms were further analyzed to characterize any Hb variants. Fructosamine levels were determined in place of HbA1c levels when unstable Hb variants were identified. Abnormal HPLC chromatograms were obtained for 160 of 42 371 samples. In 26 samples HbS was identified and HbA1c results correlated with FPG. In the remaining 134 samples HbD, Hb Louisville, Hb Las Palmas, Hb N-Baltimore, or Hb Porto Alegre were identified and HbA1c did not correlate with FPG. These samples were retested using an immunoturbidimetric assay and the majority of results were accurate; only 3 (with the unstable Hb Louisville trait) gave aberrant HbA1c results. Hb variants can affect determination of HbA1c levels with some methods. Laboratories should be aware of Hb variants occurring locally and choose an appropriate HbA1c testing method. PMID:25355712

  12. Which method better evaluates the molecular response in newly diagnosed chronic phase chronic myeloid leukemia patients with imatinib treatment, BCR-ABL(IS) or log reduction from the baseline level?

    Science.gov (United States)

    Qin, Ya-Zhen; Jiang, Qian; Jiang, Hao; Li, Jin-Lan; Li, Ling-Di; Zhu, Hong-Hu; Lai, Yue-Yun; Lu, Xi-Jing; Liu, Yan-Rong; Jiang, Bin; Huang, Xiao-Jun

    2013-09-01

    The molecular response of chronic myeloid leukemia (CML) patients to tyrosine kinase inhibitor treatment can be evaluated either by BCR-ABL mRNA levels on international scale (IS) or by log reduction from the baseline level of the laboratory. Both methods were compared in 248 newly diagnosed chronic phase CML patients treated with imatinib. The major molecular responses (MMR) obtained by both methods predict progression-free survival (PFS, all Plog reduction method, had the same PFS as MMR patients identified by both methods. The molecular responses of patients at 3 and 6 months, as evaluated by the two methods, have similar predictive values on their cytogenetic responses at 12 months and on their molecular responses at 18 months. Both ≤ 10%(IS) and ≥ 1 log reduction at 3 months and ≤ 1%(IS) at 6 months were significantly associated with PFS (P=0.0011, 0.0090, and 0.0064). The percentages of patients with BCR-ABL(IS) of ≤ 1%, >1-10%, and of >10% at 3 months and 6 months in the German CML Study IV were similar with those with corresponding BCR-ABL(IS) in our center, but was significantly different with those evaluated by the log reduction method. Therefore, the molecular response evaluated by BCR-ABL(IS) has similar trends in PFS and in response prediction, but can better differentiate patients than that by the log reduction method. Furthermore, the IS method allows comparison among molecular response results from different laboratories.

  13. Truncated hemoglobins in actinorhizal nodules of Datisca glomerata

    NARCIS (Netherlands)

    Pawlowski, K.; Jacobsen, K.R.; Alloisio, N.; Denison, R.F.; Klein, M.; Tjepkema, J.D.; Winzer, T.; Sirrenberg, A.; Guan, C.; Berry, A.M.

    2007-01-01

    Three types of hemoglobins exist in higher plants, symbiotic, non-symbiotic, and truncated hemoglobins. Symbiotic (class II) hemoglobins play a role in oxygen supply to intracellular nitrogen-fixing symbionts in legume root nodules, and in one case ( Parasponia Sp.), a non-symbiotic (class I) hemogl

  14. 21 CFR 864.7415 - Abnormal hemoglobin assay.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Abnormal hemoglobin assay. 864.7415 Section 864.7415 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED... hemoglobin assay. (a) Identification. An abnormal hemoglobin assay is a device consisting of the...

  15. 21 CFR 864.7455 - Fetal hemoglobin assay.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Fetal hemoglobin assay. 864.7455 Section 864.7455... assay. (a) Identification. A fetal hemoglobin assay is a device that is used to determine the presence... hemoglobin present. The assay may be used to detect fetal red cells in the maternal circulation or to...

  16. 21 CFR 864.7400 - Hemoglobin A2 assay.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Hemoglobin A2 assay. 864.7400 Section 864.7400 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED... assay. (a) Identification. A hemoglobin A2 assay is a device used to determine the hemoglobin A2...

  17. Lyophilized bovine hemoglobin as a possible reference material for the determination of hemoglobin derivatives in human blood

    NARCIS (Netherlands)

    Maas, BHA; Buursma, A; Ernst, RAJ; Maas, AHJ; Zijlstra, WG

    1998-01-01

    We investigated the suitability of a lyophilized bovine hemoglobin (LBH) preparation containing various fractions of oxyhemoglobin (O(2)Hb), carboxyhemoglobin (COHb), and methemoglobin (MetHb) for quality assessment in multicomponent analysis (MCA) of hemoglobin derivatives. It was demonstrated that

  18. Biofuels Baseline 2008

    Energy Technology Data Exchange (ETDEWEB)

    Hamelinck, C.; Koper, M.; Berndes, G.; Englund, O.; Diaz-Chavez, R.; Kunen, E.; Walden, D.

    2011-10-15

    The European Union is promoting the use of biofuels and other renewable energy in transport. In April 2009, the Renewable Energy Directive (2009/28/EC) was adopted that set a 10% target for renewable energy in transport in 2020. The directive sets several requirements to the sustainability of biofuels marketed in the frame of the Directive. The Commission is required to report to the European Parliament on a regular basis on a range of sustainability impacts resulting from the use of biofuels in the EU. This report serves as a baseline of information for regular monitoring on the impacts of the Directive. Chapter 2 discusses the EU biofuels market, the production and consumption of biofuels and international trade. It is derived where the feedstock for EU consumed biofuels originally come from. Chapter 3 discusses the biofuel policy framework in the EU and major third countries of supply. It looks at various policy aspects that are relevant to comply with the EU sustainability requirements. Chapter 4 discusses the environmental and social sustainability aspects associated with EU biofuels and their feedstock. Chapter 5 discusses the macro-economic effects that indirectly result from increased EU biofuels consumption, on commodity prices and land use. Chapter 6 presents country factsheets for main third countries that supplied biofuels to the EU market in 2008.

  19. TWRS privatization process technical baseline

    Energy Technology Data Exchange (ETDEWEB)

    Orme, R.M.

    1996-09-13

    The U.S. Department of Energy (DOE) is planning a two-phased program for the remediation of Hanford tank waste. Phase 1 is a pilot program to demonstrate the procurement of treatment services. The volume of waste treated during the Phase 1 is a small percentage of the tank waste. During Phase 2, DOE intends to procure treatment services for the balance of the waste. The TWRS Privatization Process Technical Baseline (PPTB) provides a summary level flowsheet/mass balance of tank waste treatment operations which is consistent with the tank inventory information, waste feed staging studies, and privatization guidelines currently available. The PPTB will be revised periodically as privatized processing concepts are crystallized.

  20. Correlations between the level of inhibition C, glycosylated hemoglobin A1c and the coronary lesions in patients with coronary heart disease%冠心病患者胱抑素C及糖化血红蛋白水平与冠脉病变的关系

    Institute of Scientific and Technical Information of China (English)

    王蒙蒙; 李恩; 汪涛

    2015-01-01

    Objective To study the correlations between the levels of inhibition C (Cys-C), glycosylated hemoglobin A1c (HbA1c) and the coronary lesion. Methods 184 patients were divided into the coronary heart disease (CHD) group (n = 113) and the control group (n = 71) according to coronary angiography. The correlations between the level of Cys-C, HbA1c and Gensini score and coronary vessel lesions were investigated. Results The levels of Cys-C and HbA1c of CHD group were significantly higher than the control group (P 6.5%)Cys-C水平与Gensini积分呈正相关性(r=0.440,P=0.002). 结论:HbA1c、Cys-C水平与冠脉病变的程度及支数呈正相关,联合检测Cys-C、HbA1c水平对冠脉病变有一定的预测价值.

  1. The LSD1 inhibitor RN-1 recapitulates the fetal pattern of hemoglobin synthesis in baboons (P. anubis).

    Science.gov (United States)

    Rivers, Angela; Vaitkus, Kestis; Ibanez, Vinzon; Ruiz, Maria Armila; Jagadeeswaran, Ramasamy; Saunthararajah, Yogen; Cui, Shuaiying; Engel, James D; DeSimone, Joseph; Lavelle, Donald

    2016-06-01

    Increased fetal hemoglobin levels lessen the severity of symptoms and increase the lifespan of patients with sickle cell disease. Hydroxyurea, the only drug currently approved for the treatment of sickle cell disease, is not effective in a large proportion of patients and therefore new pharmacological agents that increase fetal hemoglobin levels have long been sought. Recent studies identifying LSD-1 as a repressor of γ-globin expression led to experiments demonstrating that the LSD-1 inhibitor RN-1 increased γ-globin expression in the sickle cell mouse model. Because the arrangement and developmental stage-specific expression pattern of the β-like globin genes is highly conserved between man and baboon, the baboon model remains the best predictor of activity of fetal hemoglobin-inducing agents in man. In this report, we demonstrate that RN-1 increases γ-globin synthesis, fetal hemoglobin, and F cells to high levels in both anemic and non-anemic baboons with activity comparable to decitabine, the most potent fetal hemoglobin-inducing agent known. RN-1 not only restores high levels of fetal hemoglobin but causes the individual 5' Iγ- and 3' Vγ-globin chains to be synthesized in the ratio characteristic of fetal development. Increased fetal hemoglobin was associated with increased levels of acetylated Histone H3, H3K4Me2, H3K4Me3, and RNA polymerase II at the γ-globin gene, and diminished γ-globin promoter DNA methylation. RN-1 is likely to induce clinically relevant levels of fetal hemoglobin in patients with sickle cell disease, although careful titration of the dose may be required to minimize myelotoxicity. PMID:26858356

  2. Ex vivo hemoglobin status study using photoacoustic computed tomography small animal scanner

    Science.gov (United States)

    Liu, Bo; Kruger, Robert; Reinecke, Daniel; Stantz, Keith M.

    2010-02-01

    Purpose: The purpose of this study is to calibrate the PCT scanner to quantify the hemoglobin status utilizing a blood flow phantom. Materials and Methods: A blood circulation system was designed and constructed to control the oxygen saturation and hemoglobin concentration of blood. As a part of the circulation system, a 1.1mm FEP tube was placed in the center of imaging tank of PCT scanner as the imaging object. Photoacoustic spectra (690-950 nm) was acquired for different hemoglobin concentrations (CtHb) and oxygen saturation levels (SaO2), where the formers was formed by diluting blood samples with PBS and the latter by mixing blood with gases at different oxygen content. Monte Carlo simulations were performed to calculate the photon energy depositions in the phantom tube, which took into account photon losses in water and blood. A Kappa value which represents the energy transfer efficiency of hemoglobin molecule was calculated based on the PCT measurement and simulation result. The final SaO2 value of each blood sample was calculated based on the PCT spectrum and Kappa value. These oxygen saturation results were compared with co-oximeter measurements to obtain systematic errors. Results and Conclusion: The statistic error of calculating Kappa value from hemoglobin concentration experiment was less than 5%. The systematic error between PCT spectra analysis and co-oximeter analysis for hemoglobin oxygen saturation was -4.5%. These calibration techniques used to calculate Kappa and hemoglobin absorption spectra would be used in hypoxia measurements in tumors as well as for endogenous biomarkers studies.

  3. Insulin-induced lipid binding to hemoglobin

    Directory of Open Access Journals (Sweden)

    VESNA NIKETIC

    2003-01-01

    Full Text Available Under hypoglycemic conditions, concomitant hyperinsulinism causes an apparent modification of hemoglobin (Hb which is manifested by its aggregation (Niketi} et al., Clin. Chim. Acta 197 (1991 47. In the present work the causes and mechanisms underlying this Hb modification were studied. Hemoglobin isolated from normal erythrocytes incubated with insulin was analyzed by applying 31P-spectrometry and lipid extraction and analysis. To study the dynamics of the plasma membrane during hyperinsulinism, a fluorescent lipid-analog was applied. In the presence of insulin, phosphatidylserine (PS, phosphatidylethanolamine (PE and cholesterol were found to bind to Hb. Lipid binding resulted in Hb aggregation, a condition that can be reproduced when phospholipids are incubated with Hb in vitro. Using a fluorescent lipid-analog, it was also shown that exposing erythrocytes to supraphysiological concentrations of insulin in vitro resulted in the internalization of lipids. The results presented in this work may have relevance to cases of diabetes mellitus and hypoglycemia.

  4. Hemoglobin expression in rat experimental granulation tissue

    Institute of Scientific and Technical Information of China (English)

    Miretta Tommila; Christoffer Stark; Anne Jokilammi; Ville Peltonen; Risto Penttinen; Erika Ekholm

    2011-01-01

    The general opinion that hemoglobin is only a carrier protein for oxygen and carbon dioxide has been challenged by several recent studies showing hemoglobin expression in other cells than those of the erythroid series, for example, in macrophages. We discovered β-globin expression in rat experimental granulation tissue induced by subcutaneously implanted cellulose sponges. Closer investigation revealed also α-globin expression. The first peak of the biphasic globin expression noticed during granulation tissue formation correlated with the invasion of monocytes/macrophages, whereas the second one seemed to be connected to the appearance of hematopoietic progenitors. Data presented in this study indicate globin expression both in macrophages and in immature erythroid cells as validated by erythroid-specific markers.

  5. 洞庭湖区土壤地球化学基准值与污染等级划分%Soil geochemical baseline and pollution level division in Dongting lake area

    Institute of Scientific and Technical Information of China (English)

    张建新

    2014-01-01

    Deep soil and its geochemical significance in multi-objective investigation are hot topics among environmental scientists. The author compared 54 indexes and their geochemical distribution characteristics in surface soil and deep soil of Dongting plain and hillock area, and studied the element content variation from natural differentiation and anthropogenic influence in the pedogenic process. With deep soil as the reference, the author obtained regional soil geochemical background value and baseline and proposed a method for the classification of surface soil pollution levels.%通过洞庭湖平原与周边丘岗山地区深、表层土壤中54项指标的地球化学分析对比,研究成壤过程中元素的自然分异和人为叠加作用造成的土壤元素含量在垂向上的差异;以深层土壤为参照,求解区域土壤的地球化学背景值和基准值,并以此为标准提出表层土壤污染等级划分的地球化学方法。

  6. Erythropoietin, ferritin, haptoglobin, hemoglobin and transferrin receptor in metabolic syndrome: a case control study

    Directory of Open Access Journals (Sweden)

    Hämäläinen Päivi

    2012-09-01

    Full Text Available Abstract Background Increased ferritin concentrations are associated with metabolic syndrome (MetS. The association between ferritin as well as hemoglobin level and individual MetS components is unclear. Erythropoietin levels in subjects with MetS have not been determined previously. The aim of this study was to compare serum erythropoietin, ferritin, haptoglobin, hemoglobin, and transferrin receptor (sTFR levels between subjects with and without MetS and subjects with individual MetS components. Methods A population based cross-sectional study of 766 Caucasian, middle-aged subjects (341 men and 425 women from five age groups born in Pieksämäki, Finland who were invited to a health check-up in 2004 with no exclusion criteria. Laboratory analyzes of blood samples collected in 2004 were done during year 2010. MetS was defined by National Cholesterol Education Program criteria. Results 159 (53% men and 170 (40% women of study population met MetS criteria. Hemoglobin and ferritin levels as well as erythropoietin and haptoglobin levels were higher in subjects with MetS (p  Conclusion Subjects with MetS have elevated hemoglobin, ferritin, erythropoietin and haptoglobin concentrations. Higher hemoglobin levels are related to all components of MetS. Higher ferritin levels associate with TG, abdominal obesity, elevated glucose or low high density cholesterol. Haptoglobin levels associate with blood pressure or elevated glucose. However, erythropoietin levels are related only with abdominal obesity. Higher serum erythropoietin concentrations may suggest underlying adipose tissue hypoxemia in MetS.

  7. Therapeutic Depletion of Iron Stores Is Not Associated with a Reduced Hemoglobin Mass in a Hemochromatosis Patient

    Science.gov (United States)

    Wrobel, Nina; Pottgiesser, Torben; Birkner, Philipp; Deibert, Peter; Ahlgrim, Christoph

    2016-01-01

    Introduction Hereditary hemochromatosis features a dysregulated iron absorption leading to iron overload and organ damage. The regulation of total hemoglobin mass during depletion of iron deposits by therapeutic phlebotomy has not been studied. Case Presentation The initial ferritin level of the 52-year-old male subject was 1,276 μg/l. Despite successful depletion of iron stores (ferritinmin: 53 μg/l) through phlebotomies, total hemoglobin mass stabilized at the pretherapy level. However, regeneration of total hemoglobin mass was accelerated (up to 10.8 g/day). Conclusion In this hemochromatosis patient, the total hemoglobin mass was not altered in the long term, but regeneration was accelerated, possibly due to elevated body iron content. PMID:27721733

  8. Functional behavior of tortoise hemoglobin Geochelone denticulata

    OpenAIRE

    TORSONI M. A.; STOPPA G. R.; A. Turra; OGO S. H.

    2002-01-01

    The hemolysate from Geochelone denticulata contains two main hemoglobin components, as shown by ion exchange chromatography and polyacrylamide gel electrophoresis (PAGE). Electrophoresis under dissociating conditions showed three types of globin chains. The apparent molecular mass, as determined by gel filtration on Sephadex G-200, was compatible with tetrameric Hb, which was unable to polymerize. The G. denticulata Hb has a P50 value of 9.56 mm Hg at pH 7.4. The Hb oxygenation appears to be ...

  9. Facile Interfacial Electron Transfer of Hemoglobin

    Directory of Open Access Journals (Sweden)

    Chunhai Fan

    2005-12-01

    Full Text Available Abstract: We herein describe a method of depositing hemoglobin (Hb and sulfonated polyaniline (SPAN on GC electrodes that facilitate interfacial protein electron transfer. Well-defined, reproducible, chemically reversible peaks of Hb and SPAN can be obtained in our experiments. We also observed enhanced peroxidase activity of Hb in SPAN films. These results clearly showed that SPAN worked as molecular wires and effectively exchanged electrons between Hb and electrodes.Mediated by Conjugated Polymers

  10. Hemoglobin profiles of siblings of thalassemia patients

    Directory of Open Access Journals (Sweden)

    Muhammad Riza

    2015-03-01

    Full Text Available Background Thalassemia and hemoglobinopathies are the most common inherited disorders in many areas of the world, including South East Asia. The siblings of thalassemia major is a group of high risk to carry the gene of thalassemia. Determining the carrier is useful for early treatment planning and prevention to the next child. Objective To determine carrier status among siblings of thalassemia patients using a capillary electrophoresis system. Methods A cross-sectional study on the siblings of thalassemia major patients was performed from January 2011 to February 2012 at Dr. Moewardi Hospital. Complete blood counts were performed in the siblings. Subjects with mean corpuscular volume (MCV <80 fl and mean corpuscular hemoglobin (MCH <27 pg were subjected to analize hemoglobin fraction by capillary electrophoresis. Results Of the 26 subjects, there were 12 males and 14 females. The mean age was 9.38 (SD 6.8 years (range 1 to 29 years. From the siblings, 10 were identified as normal, 5 were identified as ß thalassemia carriers and 5 were hemoglobin E (HbE carriers. Six siblings were diagnosed with ß thalassemia/ HbE. Conclusion There are high occurrence of the two common types of thalassemia carriers (ß and HbE in our small group of subjects who had a family history of thalassemia. Most of the siblings of thalassemia had low MCV and MCH. [Paediatr Indones. 2015;55:70-3.].

  11. Induction of nano pore in Agrobacterial hemoglobin

    Directory of Open Access Journals (Sweden)

    Mojtaba Tousheh

    2014-01-01

    Full Text Available Introduction: A variety of oxygen-transport and -binding proteins exist in organisms including bacteria, protozoans, and fungi all have hemoglobin-like proteins. In addition to dealing with transport and sensing of oxygen, they may also deal with NO2, CO2, sulfide compounds, and even O2 scavenging in environments. Also they detoxified chlorinated materials like P450 enzymes and peroxidases and use as a detector of nitrate and hydrogen peroxide. Pore-forming bacterial globins are interested for filtration. Materials and methods: Although there are data for bacterial toxin as a filter, here we used Agrobacterial hem to induce nano pore in the heme structure using point mutation. Results: Investigations showed that three amino acids leucine 76, alanine 83 and histidine 80 are important for pore formation in Agrobacterium hemoglobin. A point mutation on leucine 76 to glycine, histidine 80 to asparagine and alanine 83 to lysine step by step led to create the nano pore 0.7- 0.8 nm in the globin. Discussion and conclusion: These mutations in bacterial hemoglobin increase the stability when mutation is with it’s at pH7. This mutation decreases the aliphatic index however increase the stability index.

  12. Monitor hemoglobin concentration and oxygen saturation in living mouse tail using photoacoustic CT scanner

    Science.gov (United States)

    Liu, Bo; Kruger, Robert; Reinecke, Daniel; Stantz, Keith M.

    2010-02-01

    Purpose: The purpose of this study is to use PCT spectroscopy scanner to monitor the hemoglobin concentration and oxygen saturation change of living mouse by imaging the artery and veins in a mouse tail. Materials and Methods: One mouse tail was scanned using the PCT small animal scanner at the isosbestic wavelength (796nm) to obtain its hemoglobin concentration. Immediately after the scan, the mouse was euthanized and its blood was extracted from the heart. The true hemoglobin concentration was measured using a co-oximeter. Reconstruction correction algorithm to compensate the acoustic signal loss due to the existence of bone structure in the mouse tail was developed. After the correction, the hemoglobin concentration was calculated from the PCT images and compared with co-oximeter result. Next, one mouse were immobilized in the PCT scanner. Gas with different concentrations of oxygen was given to mouse to change the oxygen saturation. PCT tail vessel spectroscopy scans were performed 15 minutes after the introduction of gas. The oxygen saturation values were then calculated to monitor the oxygen saturation change of mouse. Results: The systematic error for hemoglobin concentration measurement was less than 5% based on preliminary analysis. Same correction technique was used for oxygen saturation calculation. After correction, the oxygen saturation level change matches the oxygen volume ratio change of the introduced gas. Conclusion: This living mouse tail experiment has shown that NIR PCT-spectroscopy can be used to monitor the oxygen saturation status in living small animals.

  13. Thrombocytopenia and erythrocytosis in mice with a mutation in the gene encoding the hemoglobin β minor chain

    OpenAIRE

    Kauppi, Maria; Hilton, Adrienne A; Metcalf, Donald; Ng, Ashley P.; Hyland, Craig D.; Collinge, Janelle E.; Kile, Benjamin T.; Hilton, Douglas J.; Alexander, Warren S.

    2011-01-01

    Diverse mutations in the genes encoding hemoglobin (Hb) have been characterized in human disease. We describe here a mutation in the mouse Hbb-b2 gene, denoted Plt12, that precisely mimics the human hemoglobin Hotel Dieu variant. The mutation results in increased affinity of Hb for oxygen and Plt12 mutant mice exhibited reduced partial pressure of O2 in the blood, accompanied by erythrocytosis characterized by elevated erythropoietin levels and splenomegaly with excess erythropoiesis. Most ho...

  14. Thalassemia and Hemoglobin E in Southern Thai Blood Donors

    Directory of Open Access Journals (Sweden)

    Manit Nuinoon

    2014-01-01

    Full Text Available Thalassemia and hemoglobin E (Hb E are common in Thailand. Individuals with thalassemia trait usually have a normal hemoglobin concentration or mild anemia. Therefore, thalassemic individuals who have minimum acceptable Hb level may be accepted as blood donors. This study was aimed at determining the frequency of α-thalassemia 1 trait, β-thalassemia trait, and Hb E-related syndromes in Southern Thai blood donors. One hundred and sixteen voluntary blood donors, Southern Thailand origin, were recruited for thalassemia and Hb E screening by red blood cell indices/dichlorophenolindophenol precipitation test. β-Thalassemia and Hb E were then identified by high performance liquid chromatography and 4 common α-thalassemia deletions were characterized by a single tube-multiplex gap-polymerase chain reaction. Overall frequency of hemoglobinopathies was 12.9%, classified as follows: homozygous α-thalassemia 2 (1.7%, heterozygous α-thalassemia 1 (1.7%, heterozygous β-thalassemia without α-thalassemia (0.9%, heterozygous Hb E without α-thalassemia (5.2%, double heterozygotes for Hb E/α-thalassemia 1 (1.7%, homozygous Hb E without α-thalassemia (0.9%, and homozygous Hb E with heterozygous α-thalassemia 2 (0.9%. The usefulness of thalassemia screening is not only for receiving highly effective red blood cells in the recipients but also for encouraging the control and prevention program of thalassemia in blood donors.

  15. Blood lead levels, δ-ALAD inhibition, and hemoglobin content in blood of giant toad (Rhinella marina) to assess lead exposure in three areas surrounding an industrial complex in Coatzacoalcos, Veracruz, Mexico.

    Science.gov (United States)

    Ilizaliturri-Hernández, César Arturo; González-Mille, Donaji Josefina; Mejía-Saavedra, Jesús; Espinosa-Reyes, Guillermo; Torres-Dosal, Arturo; Pérez-Maldonado, Iván

    2013-02-01

    The Coatzacoalcos Region in Veracruz, Mexico houses one of the most important industrial complexes in Mexico and Latin America. Lead is an ubiquitous environmental pollutant which represents a great risk to human health and ecosystems. Amphibian populations have been recognized as biomonitors of changes in environmental conditions. The purpose of this research is to measure exposure to lead and evaluate hematological and biochemical effects in specimens of giant toads (Rhinella marina) taken from three areas surrounding an industrial complex in the Coatzacoalcos River downstream. Lead levels in toads' blood are between 10.8 and 70.6 μg/dL and are significantly higher in industrial sites. We have found a significant decrease in the delta-aminolevulinic acid dehydratase (δ-ALAD) activity in blood from 35.3 to 78 % for the urban-industrial and industrial sites, respectively. In addition, we have identified a strong inverse relationship between the δ-ALAD activity and the blood lead levels (r = -0.84, p marina can be considered a good biomonitor of the δ-ALAD activity inhibition and hematological alterations at low lead concentrations. PMID:22580791

  16. Hemoglobin cut-off values in healthy Turkish infants

    Institute of Scientific and Technical Information of China (English)

    Ahmet Arvas; Emel Gür; DurmuşDoğan

    2014-01-01

    Background: Anemia is a widespread public health problem associated with an increased risk of morbidity and mortality. This study was undertaken to determine the cut-off value of hemoglobin for infant anemia. Methods: A cross-sectional retrospective study was carried out at well-baby clinics of a tertiary care hospital. A total of 1484 healthy infants aged between 4 to 24 months were included in the study. The relationship of hemoglobin (Hb) levels with mother age, birth weight, weight gain rate, feeding, and gender was evaluated. Results: The Hb levels were assessed in four age groups (4 months, 6 months, 9-12 months, and 15-24 months) and the cut-off values of Hb were determined. Hb cut-off values (5th percentile for age) were detected as 97 g/L and 93 g/L at 4 months and 6 months, respectively. In older infants, the 5th percentile was 90.5 g/L and 93.4 g/L at 9-12 months and 15-24 months, respectively. The two values were lower than the World Health Organization criteria for anemia, which could partly due to the lack of information on iron status in our population. However, this difference highlights the need for further studies on normal Hb levels in healthy infants in developing countries. Hb levels of females were higher in all age groups; however, a statistically significant difference was found in gender in only 6 month-old infants. No statistically significant difference was found among Hb levels, mother's age, birth weight, weight gain rate, and nutritional status. Conclusion: Hb cut-off values in infants should be re-evaluated and be compatible with growth and development of children in that community.

  17. Hemoglobins: models of physiological adaptation, with special reference to O2 availability and temperature

    DEFF Research Database (Denmark)

    Weber, Roy E.

    In transporting O2 from the respiratory surfaces to the respiring tissues of animals, hemoglobin (Hb) directly links aerobic metabolism with O2 availability and is a paradigm for studying mechanisms of molecular adaptations. Hb-O2 binding is cooperative (described by sigmoid O2 binding curves...... of compensatory adjustments at molecular, cellular and organismic levels of organization....

  18. Hanford Site technical baseline database

    Energy Technology Data Exchange (ETDEWEB)

    Porter, P.E., Westinghouse Hanford

    1996-05-10

    This document includes a cassette tape that contains the Hanford specific files that make up the Hanford Site Technical Baseline Database as of May 10, 1996. The cassette tape also includes the delta files that delineate the differences between this revision and revision 3 (April 10, 1996) of the Hanford Site Technical Baseline Database.

  19. Hanford Site technical baseline database

    Energy Technology Data Exchange (ETDEWEB)

    Porter, P.E.

    1996-09-30

    This document includes a cassette tape that contains the Hanford specific files that make up the Hanford Site Technical Baseline Database as of September 30, 1996. The cassette tape also includes the delta files that dellinate the differences between this revision and revision 4 (May 10, 1996) of the Hanford Site Technical Baseline Database.

  20. Baseline Familiarity in Lie Detection.

    Science.gov (United States)

    Feeley, Thomas H.; And Others

    1995-01-01

    Reports on a study in which subjects judged the veracity of truthful and deceptive communicators after viewing no, one, two, or four case-relevant baseline exposures (familiarity) of truthful communication. Finds a positive linear relationship between detection accuracy and amount of baseline familiarity. (SR)

  1. Curcumin Supplementation Lowers TNF-α, IL-6, IL-8, and MCP-1 Secretion in High Glucose-Treated Cultured Monocytes and Blood Levels of TNF-α, IL-6, MCP-1, Glucose, and Glycosylated Hemoglobin in Diabetic Rats

    OpenAIRE

    Jain, Sushil K.; Rains, Justin; Croad, Jennifer; Larson, Bryon; Jones, Kimberly

    2009-01-01

    This study examined the hypothesis that curcumin supplementation decreases blood levels of IL-6, MCP-1, TNF-α, hyperglycemia, and oxidative stress by using a cell-culture model and a diabetic rat model. U937 monocytes were cultured with control (7 mM) and high glucose (35 mM) in the absence or presence of curcumin (0.01–1 μM) at 37°C for 24 h. Diabetes was induced in Sprague–Dawley rats by injection of streptozotocin (STZ) (i.p., 65 mg/kg BW). Control buffer, olive oil, or curcumin (100 mg/kg...

  2. Program cost and schedule baseline

    International Nuclear Information System (INIS)

    The overall purpose of cost and schedule baseline management is to ensure that the Civilian Radioactive Waste Management (OCRWM) Program's cost and schedule plan are controlled in an orderly, efficient, and documented manner. Baseline control of the Program's cost and schedule will result in changes that only take place with OCRWM senior management approval, by way of the change control process. The Program Management System (PMS) Manual describes how the Program Cost and Schedule Baseline (PCSB) is integrated into the OCRWM management system. This PCSB document identifies the components of Program cost and schedule that will be subject to change control by DOE-Headquarters and establishes their baseline values. This document also summarizes the management components of the cost and schedule baseline, including reporting, monitoring, and corrective action. Detailed PCSB reporting, monitoring, and corrective action procedures will be issued as an Office of Program Administration and Resource Management (OPARM) administrative procedure. 9 figs

  3. Plutonium Immobilization Project Baseline Formulation

    Energy Technology Data Exchange (ETDEWEB)

    Ebbinghaus, B.

    1999-02-01

    A key milestone for the Immobilization Project (AOP Milestone 3.2a) in Fiscal Year 1998 (FY98) is the definition of the baseline composition or formulation for the plutonium ceramic form. The baseline formulation for the plutonium ceramic product must be finalized before the repository- and plant-related process specifications can be determined. The baseline formulation that is currently specified is given in Table 1.1. In addition to the baseline formulation specification, this report provides specifications for two alternative formulations, related compositional specifications (e.g., precursor compositions and mixing recipes), and other preliminary form and process specifications that are linked to the baseline formulation. The preliminary specifications, when finalized, are not expected to vary tremendously from the preliminary values given.

  4. Atmospheric media effects on ARIES baseline determination

    Science.gov (United States)

    Wu, S. C.

    1981-01-01

    Different types of media effects on ARIES baseline determination are compared. The effectiveness of simple ionospheric calibration models are studied. To perform the covariance analysis, an ARIES observation sequence needs to be assumed. For the current purposes, the observation sequence is selected to be that of experiment 80D over the JPL/Goldstone baseline (approximately 180 km). This experiment consisted of 96 observations over a period of approximately 25 hours on March 25 to 26, 1980. It is found through covariance analyses that the component most sensitive to media depends heavily upon the correlation, between the two stations, of the media effects. It is also found that relying on the cancellation of ionospheric delays between the two ray paths of VLBI observations at S band results in a large error in baseline length determination. High degree removal of ionospheric effects is possible with a crude model, providing correct diurnal peak and minimum ionospheric levels are input.

  5. Fetal hemoglobin reactivation in baboon and man: a short perspective.

    Science.gov (United States)

    Lavelle, D; DeSimone, J; Heller, P

    1993-01-01

    Present concepts of the mechanism of reactivation of synthesis of fetal hemoglobin (HbF) in the adult under conditions of erythropoietic stress are briefly reviewed. Since HbF can be considered an effective natural antisickling agent, the reactivation of its synthesis in patients with sickle cell anemia as a desirable therapeutic goal has been extensively explored since the discovery in 1982 that 5-azacytidine increases HbF levels in the baboon. Hydroxyurea (HU) has become the most widely used agent, although its effectiveness in increasing HbF levels and the number of F cells is highly variable. Recent investigations are cited showing that other agents such as butyrate, and the addition of recombinant hemopoietic growth factors, such as erythropoietin and stem cell factor, especially in combination with HU, offer important therapeutic possibilities. Transacting nuclear proteins are briefly discussed as possibly having a future role in the efforts of stimulating gamma-chain synthesis. PMID:7677951

  6. Estimation of prevalence of Anemia using WHO hemoglobin color scale among Non pregnant females of urban slum

    Directory of Open Access Journals (Sweden)

    Satish Saroshe

    2014-05-01

    Full Text Available Background: Nutritional anemia is a major public health problem worldwide particularly in developing countries among women of reproductive age. WHO Hemoglobin Color Scale is easy, quick and handy technique to estimate hemoglobin level at field. Objective: To find out prevalence of anemia using WHO hemoglobin color scale among the females of reproductive age group. To find out the most common signs and symptoms associated with anemia. To find out the causes associated with anemia among females. Material& Methods: A cross sectional study with written informed consent was conducted in 400 females of an urban slum area of Indore city. Females were selected using systematic random sampling method. All the females of reproductive age group were included in study. Level of hemoglobin was obtaining using WHO Hemoglobin color scale. A questionnaire was used during interpersonal interview of all the anemic females which was followed by clinical examination to assess signs and symptoms associated with anemia. The data was analyzed using Microsoft office excel sheet. Results: 61% of females of reproductive age group were found to be anemic by hemoglobin color scale. 54 % complained of frequent headache, 50 % of difficulty in breathing during normal work and 49.18% of reduced appetite. Conclusion: Anemia is found more in females of reproductive age group in urban slum. Most common

  7. Impact of hemoglobin nitrite to nitric oxide reductase on blood transfusion for resuscitation from hemorrhagic shock

    Directory of Open Access Journals (Sweden)

    Chad Brouse

    2015-01-01

    Full Text Available Background: Transfusion of blood remains the gold standard for fluid resuscitation from hemorrhagic shock. Hemoglobin (Hb within the red blood cell transports oxygen and modulates nitric oxide (NO through NO scavenging and nitrite reductase. Aims: This study was designed to examine the effects of incorporating a novel NO modulator, RRx-001, on systemic and microvascular hemodynamic response after blood transfusion for resuscitation from hemorrhagic shock in a hamster window chamber model. In addition, to RRx-001 the role of low dose of nitrite (1 × 10−9 moles per animal supplementation after resuscitation was studied. Materials and Methods: Severe hemorrhage was induced by arterial controlled bleeding of 50% of the blood volume (BV and the hypovolemic state was maintained for 1 h. The animals received volume resuscitation by an infusion of 25% of BV using fresh blood alone or with added nitrite, or fresh blood treated with RRx-001 (140 mg/kg or RRx-001 (140 mg/kg with added nitrite. Systemic and microvascular hemodynamics were followed at baseline and at different time points during the entire study. Tissue apoptosis and necrosis were measured 8 h after resuscitation to correlate hemodynamic changes with tissue viability. Results: Compared to resuscitation with blood alone, blood treated with RRx-001 decreased vascular resistance, increased blood flow and functional capillary density immediately after resuscitation and preserved tissue viability. Furthermore, in RRx-001 treated animals, both mean arterial pressure (MAP and met Hb were maintained within normal levels after resuscitation (MAP >90 mmHg and metHb <2%. The addition of nitrite to RRx-001 did not significantly improve the effects of RRx-001, as it increased methemoglobinemia and lower MAP. Conclusion: RRx-001 alone enhanced perfusion and reduced tissue damage as compared to blood; it may serve as an adjunct therapy to the current gold standard treatment for resuscitation from

  8. [Structural anomalies of dog hemoglobin after ionizing irradiation].

    Science.gov (United States)

    Sukhomlinov, B F; Savich, A V; Shal'no, M I; Starikovich, L S; Dudok, E P

    1981-01-01

    Heterogeneity of dog hemoglobin is established with application of chromatographic analysis. Ionizing radiation (4 Grey) induces no changes in the ratio of hemoglobin components. The comparative dactylographical analysis of the hemoglobin components in norm and in different periods after irradiation revealed differences in responses to tryptophan in peptide T-25. The changes found are connected with disturbances in the structure of the DNA molecule as well as with modification of the protein molecule under conditions of radiation injury. PMID:7324186

  9. Challenges in HbA1c Analysis and Reporting in Patients with Variant Hemoglobins.

    Science.gov (United States)

    Sultana, T A; Sheme, Z A; Sultana, G S; Sultana, B; Mishu, F A; Khan, N Z; Sarkar, B C; Muttalib, M A; Khan, S A; Choudhury, S; Mahtab, H

    2016-04-01

    Hemoglobin A1c (HbA1c) is a well-established indicator of mean glycemia. The presence of genetic variants of hemoglobin can profoundly affect the accuracy of HbA1c measurements. Variants of hemoglobin especially Hemoglobin E (HbE) is prevalent in South East Asia including Bangladesh. The objective of our study is to compare the HbA1c values measured on high performance liquid chromatography (HPLC) and Turbidimetric Inhibition Immunoassay (TINIA) in diabetic patients with variant hemoglobins including HbE. A total of 7595 diabetic patients receiving treatment at BIRDEM General Hospital were analyzed for HbA1c results within a period of two months from December 2013 to January 2014. Seventy two cases out of 7595 (0.95%) had either undetectable or below normal HbA1c levels (males-33 and females-39; ratio = 0.82:1) by HPLC method. In 34(0.45%) cases, HbA1c value was undetectable by HPLC method but was in the reportable range by TINIA method. In the other 38 (0.55%) cases, HbA1c levels were below the reportable range (levels, [Mean bias -5.2(-9.3 to 1.0), 95% CI] but agreed very well [mean bias -0.21 (-0.84 to 0.42), y=1.1037+0.776X; r2=0.30, pblood sugar levels of all the 72 cases correlated strongly with TINIA method (r2 =0.75, plevel is essential. MCV of 80fl or below may serve as a rough guide to select samples that require analysis by TINIA method. Moreover, HPLC may be a convenient and inexpensive tool for screening of hemoglobinopathies especially among diabetic population in Bangladesh. It may therefore be helpful in improving management of complications related to both anaemia and iron overload. PMID:27277356

  10. Baseline methodologies for clean development mechanism projects

    International Nuclear Information System (INIS)

    The Kyoto Protocol and the Clean Development Mechanism (CDM) came into force on 16th February 2005 with its ratification by Russia. The increasing momentum of this process is reflected in more than 100 projects having been submitted to the CDM Executive Board (CDM-EB) for approval of the baselines and monitoring methodologies, which is the first step in developing and implementing CDM projects. A CDM project should result in a net decrease of GHG emissions below any level that would have resulted from other activities implemented in the absence of that CDM project. The 'baseline' defines the GHG emissions of activities that would have been implemented in the absence of a CDM project. The baseline methodology is the process/algorithm for establishing that baseline. The baseline, along with the baseline methodology, are thus the most critical element of any CDM project towards meeting the important criteria of CDM, which are that a CDM should result in 'real, measurable, and long term benefits related to the mitigation of climate change'. This guidebook is produced within the frame work of the United Nations Environment Programme (UNEP) facilitated 'Capacity Development for the Clean Development Mechanism (CD4CDM)' Project. This document is published as part of the projects effort to develop guidebooks that cover important issues such as project finance, sustainability impacts, legal framework and institutional framework. These materials are aimed to help stakeholders better understand the CDM and are believed to eventually contribute to maximize the effect of the CDM in achieving the ultimate goal of UNFCCC and its Kyoto Protocol. This Guidebook should be read in conjunction with the information provided in the two other guidebooks entitled, 'Clean Development Mechanism: Introduction to the CDM' and 'CDM Information and Guidebook' developed under the CD4CDM project. (BA)

  11. Baseline methodologies for clean development mechanism projects

    Energy Technology Data Exchange (ETDEWEB)

    Lee, M.K. (ed.); Shrestha, R.M.; Sharma, S.; Timilsina, G.R.; Kumar, S.

    2005-11-15

    The Kyoto Protocol and the Clean Development Mechanism (CDM) came into force on 16th February 2005 with its ratification by Russia. The increasing momentum of this process is reflected in more than 100 projects having been submitted to the CDM Executive Board (CDM-EB) for approval of the baselines and monitoring methodologies, which is the first step in developing and implementing CDM projects. A CDM project should result in a net decrease of GHG emissions below any level that would have resulted from other activities implemented in the absence of that CDM project. The 'baseline' defines the GHG emissions of activities that would have been implemented in the absence of a CDM project. The baseline methodology is the process/algorithm for establishing that baseline. The baseline, along with the baseline methodology, are thus the most critical element of any CDM project towards meeting the important criteria of CDM, which are that a CDM should result in 'real, measurable, and long term benefits related to the mitigation of climate change'. This guidebook is produced within the frame work of the United Nations Environment Programme (UNEP) facilitated 'Capacity Development for the Clean Development Mechanism (CD4CDM)' Project. This document is published as part of the projects effort to develop guidebooks that cover important issues such as project finance, sustainability impacts, legal framework and institutional framework. These materials are aimed to help stakeholders better understand the CDM and are believed to eventually contribute to maximize the effect of the CDM in achieving the ultimate goal of UNFCCC and its Kyoto Protocol. This Guidebook should be read in conjunction with the information provided in the two other guidebooks entitled, 'Clean Development Mechanism: Introduction to the CDM' and 'CDM Information and Guidebook' developed under the CD4CDM project. (BA)

  12. 324 Building Baseline Radiological Characterization

    Energy Technology Data Exchange (ETDEWEB)

    R.J. Reeder, J.C. Cooper

    2010-06-24

    This report documents the analysis of radiological data collected as part of the characterization study performed in 1998. The study was performed to create a baseline of the radiological conditions in the 324 Building.

  13. Quivira NWR biological baseline data

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This dataset is biological baseline data for Quivira National Wildlife Refuge as of January 2016. It contains data on species found on the refuge, when and where...

  14. Stability of Blood Samples for Hemoglobin Electrophoresis

    Directory of Open Access Journals (Sweden)

    Yadira Valdés Fraser

    2013-07-01

    Full Text Available Background: the National Medical Genetics Center has conducted the prenatal screening for hemoglobinopathies in the province of Artemisa and the quality control of this program nationwide; reliability of the results is determined by the quality of the samples used. Objective: to describe the stability of whole blood samples using EDTAK2 and heparin as anticoagulants. Methods: a descriptive study of 100 samples of whole blood from pregnant women and their husbands was conducted at the National Medical Genetics Center. Hemoglobin electrophoresis with Hydrasis technology was performed using 10 % EDTAK2, 2.2 % and 5 % heparin, temperature at 4-8 0C and shelf-life of 7.15 and 30 days. Results: samples with EDTAK2 showed stability for a month with accuracy and repeatability in the electrophoresis runs. By using 5 % and 2.2 % heparin, problems were found in all periods analyzed. Conclusions: 10 % EDTAK2 anticoagulant is appropriate to ensure the reliability of the results in the screening for hemoglobinopathies. The results obtained in this study can be applied in all clinical, hematological and hemoglobin electrophoresis laboratories.

  15. Learning to Baseline Business Technology

    OpenAIRE

    David Gore; Marie D. Lee; Kimberly Hopper

    2013-01-01

    bills, sign multi-­‐year contracts, and make purchasing decisions without having an overall technology plan. That plan includes a technology baseline to fully assess existing technology. A CIO's goal is to align IT with business goals. Businesses must know total cost of ownership and the return on investment for all technology purchases and monthly costs. A business must also be able to manage technology assets and best utilize resources across the business. Teaching students to baseline tech...

  16. [Influence of mastication on the amount of hemoglobin in human brain tissue].

    Science.gov (United States)

    Sasaki, A

    2001-03-01

    The purpose of this study was to investigate the influence of mastication on the amount of hemoglobin in human brain tissue. Nine healthy volunteers (6 males and 3 females) participated in this study. They underwent two tasks: 1) at rest, 2) gum-chewing. In seven of the nine (4 males and 3 females), experimental occlusal interference was applied to the first molar of the mandibule on the habitual masticatory side. They underwent the gum-chewing task. To evaluate the amount of hemoglobin, both the hemoglobin oxygenation state and blood volume during gum-chewing were measured in the frontal region, using near-infrared spectroscopy. The amount of total-hemoglobin (blood volume) and oxyhemoglobin of subjects significantly increased during gum-chewing (p < 0.01). When the subjects finished gum-chewing, both levels returned to the original levels. When experimental occlusal interference was imposed on the subject, the amount of them significantly decreased compared with subjects without experimental occlusal interference (p < 0.05). The results suggested that increases of cerebral blood flow in the frontal region were not due to the mandibular movement, and that human brain activity caused by mastication was not only in the cortical masticatory area but also in the frontal region.

  17. Comparison of Hemoglobin A1c assay performance on two different commercial systems

    Directory of Open Access Journals (Sweden)

    Jozo Ćorić

    2015-04-01

    Full Text Available Introduction: Glycated hemoglobin (HbA1c is formed by non-enzymatic binding of glucose to the free amino group of the N-terminal end of the ß-chain of hemoglobin A. HbA1c is representative of the mean blood glucose level over three months. The aim of the study was to evaluate the Hemoglobin A1c immunoturbidimetric assay performance on two different commercial systems.Methods: We evaluated the precision and trueness for determination of HbA1c in whole blood. Concentrations of total hemoglobin and HbA1c were evaluated on Dimension Xpand (Siemens and Cobas 501 (Roche analyzers. HbA1c was measured in a latex agglutination inhibition test. Commercial controls Liquichek Diabetes Control Level 1 and Liquichek Diabetes Control Level 2 (Bio Rad at two levels were used for quality control. Analytical validation of HbA1c included: within-run imprecision, between-day imprecision, inaccuracy and comparison determination on the human samples on 2 systems: Dimension Xpand and Cobas 501 analyzers. Results: Within-run imprecision on the commercially controls for Level 1 is 4.5% and Level 2 is 3.2% between-day imprecision on commercially controls is 6.1% Level 1 and 5.1% Level 2 for respectively inac- curacy on commercially controls for Level 1 is 1.8% and Level 2 is 4.8%. Method comparison on human samples shows the correlation coefficient of 0.99.Conclusion: The presented results of the analytical evaluation methods for the determination of HbA1c showed an acceptable accuracy and precision.

  18. Serum iron concentration, but not hemoglobin, correlates with TIMI risk score and 6-month left ventricular performance after primary angioplasty for acute myocardial infarction.

    Directory of Open Access Journals (Sweden)

    Ching-Hui Huang

    Full Text Available Anemia is associated with high mortality and poor prognosis after acute coronary syndrome (ACS. Increased red cell distribution width (RDW is a strong independent predictor for adverse outcomes in ACS. The common underlying mechanism for anemia and increased RDW value is iron deficiency. It is not clear whether serum iron deficiency without anemia affects left ventricular (LV performance after primary angioplasty for acute myocardial infarction (AMI. We investigated the prognostic value of serum iron concentration on LV ejection fraction (EF at 6 months and its relationship to thrombolysis in myocardial infarction (TIMI risk score in post MI patients.We recruited 55 patients who were scheduled to undergo primary coronary balloon angioplasty after AMI and 54 age- and sex-matched volunteers. Serum iron concentration and interleukin-6 levels were measured before primary angioplasty. LVEF was measured by echocardiography at baseline and after 6 months. TIMI risk score was calculated for risk stratification.Serum iron concentration was significantly lower in those in whom LVEF had not improved ≥ 10% from baseline (52.7 ± 24.1 versus 80.8 ± 50.8 µg/dl, P = 0.016 regardless of hemoglobin level, and was significantly lower in the AMI group than in the control group (62.5 ± 37.7 versus 103.0 ± 38.1 µg/dl, P<0.001. Trend analysis revealed that serum iron concentration decreased as TIMI risk score increased (P = 0.002. In addition, lower serum iron concentrations were associated with higher levels of inflammatory markers. Multiple linear regression showed that baseline serum iron concentration can predict LV systolic function 6 months after primary angioplasty for AMI even after adjusting for traditional prognostic factors.Hypoferremia is not only a marker of inflammation but also a potential prognostic factor for LV systolic function after revascularization therapy for AMI, and may be a novel biomarker for therapeutic intervention.

  19. Hepcidin level predicts hemoglobin concentration in individuals undergoing repeated phlebotomy

    OpenAIRE

    Mast, Alan E.; Schlumpf, Karen S.; Wright, David J.; Johnson, Bryce; Glynn, Simone A.; Busch, Michael P; Olbina, Gordana; Westerman, Mark; Nemeth, Elizabeta; Ganz, Tomas

    2013-01-01

    Dietary iron absorption is regulated by hepcidin, an iron regulatory protein produced by the liver. Hepcidin production is regulated by iron stores, erythropoiesis and inflammation, but its physiology when repeated blood loss occurs has not been characterized. Hepcidin was assayed in plasma samples obtained from 114 first-time/reactivated (no blood donations in preceding 2 years) female donors and 34 frequent (≥3 red blood cell donations in preceding 12 months) male donors as they were phlebo...

  20. Prognostic importance of hemoglobin in hypertensive patients with electrocardiographic left ventricular hypertrophy: the Losartan Intervention For End point reduction in hypertension (LIFE) study

    DEFF Research Database (Denmark)

    Olsen, Michael Hecht; Wachtell, Kristian; Beevers, Gareth;

    2008-01-01

    baseline hemoglobin measurements were randomized to losartan- or atenolol-based treatment and followed for 4.8 years for end points of all-cause mortality and composite of cardiovascular death, nonfatal stroke, or nonfatal myocardial infarction. RESULTS: U-shaped relations were observed between deciles.......53, 95% CI 1.27-1.85, both P or =15.0/16.2 g/dL) was not. The decrease in hemoglobin was higher (P losartan- (14.3-13.8 g/dL) versus atenolol-based treatment (14.3-14.0 g/dL). In Cox models...

  1. The relationship between gingival index and glycosylated hemoglobin level in patients with type 2 diabetes%2型糖尿病患者牙龈指数与糖化血红蛋白水平关系的研究

    Institute of Scientific and Technical Information of China (English)

    师天鹏; 牛忠英; 许樟荣; 汤楚华; 卢怡; 王小艳

    2012-01-01

    Objective: To study the relationship between blood glucose control and gingival status in patients with type 2 diabetes. Methods: According to glycosylated hemoglobin value, all the patients with type 2 diabetes mellitus were divided into three groups: good (HbAlc7.5). The variation tendency of gingival index with blood glucose control level was studied by the Chi-square test. Results; The composition of age and course of diabetes, brushing habits and simplified calculus index level were not significantly difference among the groups (P > 0. 05). The gingival index was increased with blood glucose level(P=0.001). Conclusion; The blood glucose control level is related to the gingival tissue health of the patients with type 2 diabetes.%目的:探讨2型糖尿病患者血糖控制水平与牙龈健康状况的关系,为2型糖尿病患者的牙周保健提供临床依据.方法:以2型糖尿病患者为研究对象,按糖化血红蛋白(HbAlC)值分为3组,血糖控制良好组(HbAlc7.5,55例),用卡方检验分析受试者牙龈指数等级随血糖控制水平变化的趋势.结果:各组受试者年龄构成、2型糖尿病病程基本相同(P>0.05),刷牙习惯和简化牙石指数等级无统计学差异(P>0.05),血糖控制程度差的患者牙龈指数等级高(P=0.001),其牙龈组织的炎症程度重.结论:血糖控制水平与牙周组织健康状况呈正相关关系.

  2. Molecular evolution of hemoglobins of Antarctic fishes (Notothenioidei)

    NARCIS (Netherlands)

    Stam, W.T.; Beintema, J.J; D Avino, R.; Tamburrini, M.; di Prisco, G.

    1997-01-01

    Amino acid sequences of alpha- and beta-chains of human hemoglobin and of hemoglobins of coelacanth and 24 teleost fish species, including 11 antarctic and two temperate Notothenioidei, were analyzed using maximum parsimony. Trees were derived for the alpha- and beta-chains separately and for tandem

  3. Characterization of the hemoglobins of the neonatal brushtailed possum Trichosurus vulpecula (Kerr): evidence for a highly cooperative, aggregated isoform of hemoglobin.

    Science.gov (United States)

    Henty, Kristen; Wells, Rufus M G; Brittain, Thomas

    2008-05-01

    The red blood cells of the neonatal brushtailed possum exhibit unusually strong cooperativity at high levels of oxygen saturation (n=5.4) which appear to arise from a concentration dependent aggregation of one of the neonatal hemoglobin isoforms. Red blood cells from neonatal pouched young exhibit a Bohr factor of -0.36. Stripped hemolysate is sensitive to added 2,3-bisphosphoglycerate (BPG) (apparent binding constant K=35 micromol L(-1)) and ATP (K=180 micromol L(-1)), but is largely insensitive towards chloride ions. Five isoforms of non-adult hemoglobin were identified using isoelectric focusing. Mass spectrometry indicated that two early isoforms contain alpha chains identical to the adult alpha chain. The remaining three isoforms are composed of identical alpha type and beta type gene products, but differ in their isoelectric points due to differential post-translational modification. PMID:18420437

  4. Adair-based hemoglobin equilibrium with oxygen, carbon dioxide and hydrogen ion activity.

    Science.gov (United States)

    Mateják, Marek; Kulhánek, Tomáš; Matoušek, Stanislav

    2015-04-01

    As has been known for over a century, oxygen binding onto hemoglobin is influenced by the activity of hydrogen ions (H⁺), as well as the concentration of carbon dioxide (CO₂). As is also known, the binding of both CO₂and H⁺ on terminal valine-1 residues is competitive. One-parametric situations of these hemoglobin equilibria at specific levels of H⁺, O₂or CO₂are also well described. However, we think interpolating or extrapolating this knowledge into an 'empirical' function of three independent variables has not yet been completely satisfactory. We present a model that integrates three orthogonal views of hemoglobin oxygenation, titration, and carbamination at different temperatures. The model is based only on chemical principles, Adair's oxygenation steps and Van't Hoff equation of temperature dependences. Our model fits the measurements of the Haldane coefficient and CO₂hemoglobin saturation. It also fits the oxygen dissociation curve influenced by simultaneous changes in H⁺, CO₂and O₂, which makes it a strong candidate for integration into more complex models of blood acid-base with gas transport, where any combination of mentioned substances can appear.

  5. Combinatorics of giant hexagonal bilayer hemoglobins.

    Science.gov (United States)

    Hanin, L G; Vinogradov, S N

    2000-01-01

    The paper discusses combinatorial and probabilistic models allowing to characterize various aspects of spacial symmetry and structural heterogeneity of the giant hexagonal bilayer hemoglobins (HBL Hb). Linker-dodecamer configurations of HBL are described for two and four linker types (occurring in the two most studied HBL Hb of Arenicola and Lumbricus, respectively), and the most probable configurations are found. It is shown that, for HBL with marked dodecamers, the number of 'normal-marked' pairs of dodecamers in homological position follows a binomial distribution. The group of symmetries of the dodecamer substructure of HBL is identified with the dihedral group D6. Under natural symmetry assumptions, the total dipole moment of the dodecamer substructure of HBL is shown to be zero. Biological implications of the mathematical findings are discussed.

  6. Effect of non-surgical periodontal therapy on level of serum soluble intercellular adhesion molecule-1 and glycated hemoglobin A1c in patients with type 2 diabetes and chronic periodontitis%牙周非手术治疗对2型糖尿病伴牙周炎患者糖化血红蛋白A1c和血清可溶性细胞间黏附分子-1的影响

    Institute of Scientific and Technical Information of China (English)

    袁堂霞; 张彦表; 周云; 王凡涛; 王峰

    2013-01-01

    Objective To evaluate the effects of non-surgical periodontal treatment on clinical periodontal measurements,glycemic control,and level of serum soluble intercellular adhesion molecule-1 (sICAM-1) in type 2 diabetes mellitus with chronic periodontitis patients.Methods Patients with type 2 diabetes and chronic periodontitis were selected and classified into well-controlled group[glycated hemoglobin A1c(GHbA1c)<7.00%,n=30,DMCP1 group] and poorly-controlled group (GHbA1c≥ 7.00%,n=30,DMCP2 group).Thirty systemically healthy patients with chronic periodontitis were recruited as control group (CP group),All subjects underwent non-surgical periodontal therapy.Plaque index(PLI),sulcus bleeding index (SBI),bleeding on probing(BOP),probing depth (PD),clinical attachment loss (CAL),serum sICAM-1 concentration,and the value of fasting plasma glucose(FPG),GHbA1c were recorded at baseline,1 and 3 months after periodontal treatment.Results The three study groups showed significant improvements for the levels of PD,SBI,PLI,BOP,and serum sICAM-1 concentration at 1 and 3 months after non-surgical periodontal treatment (P<0.05).The level of CP group and DMCP1 group also showed significant improvements for the levels of CAL(P<0.05),but no significant change was found in DMCP2 group(P>0.05).At 3 months after periodontal treatment,GHbA1c levels in DMCP2 group significantly decreased by 1.12%(P<0.05),whereas no significant changes were found in CP and DMCP1 groups (P>0.05).Conclusion Non-surgical periodontal treatment can significantly improve periodontal health status in patients with type 2 diabetes and periodontitis,reduce the level of serum sICAM-1,and can reduce the level of GHbA1c in poorly controlled type 2 diabetic patients.%目的 探讨牙周非手术治疗对2型糖尿病伴慢性牙周炎(DMCP)患者牙周状况、糖代谢及血清可溶性细胞间黏附分子-1 (sICAM-1)的影响.方法 选择诊断为2型糖尿病伴慢性牙周炎的

  7. Low modulus biomimetic microgel particles with high loading of hemoglobin.

    Science.gov (United States)

    Chen, Kai; Merkel, Timothy J; Pandya, Ashish; Napier, Mary E; Luft, J Christopher; Daniel, Will; Sheiko, Sergei; DeSimone, Joseph M

    2012-09-10

    We synthesized extremely deformable red blood cell-like microgel particles and loaded them with bovine hemoglobin (Hb) to potentiate oxygen transport. With similar shape and size as red blood cells (RBCs), the particles were fabricated using the PRINT (particle replication in nonwetting templates) technique. Low cross-linking of the hydrogel resulted in very low mesh density for these particles, allowing passive diffusion of hemoglobin throughout the particles. Hb was secured in the particles through covalent conjugation of the lysine groups of Hb to carboxyl groups in the particles via EDC/NHS coupling. Confocal microscopy of particles bound to fluorescent dye-labeled Hb confirmed the uniform distribution of Hb throughout the particle interior, as opposed to the surface conjugation only. High loading ratios, up to 5 times the amount of Hb to polymer by weight, were obtained without a significant effect on particle stability and shape, though particle diameter decreased slightly with Hb conjugation. Analysis of the protein by circular dichroism (CD) spectroscopy showed that the secondary structure of Hb was unperturbed by conjugation to the particles. Methemoglobin in the particles could be maintained at a low level and the loaded Hb could still bind oxygen, as studied by UV-vis spectroscopy. Hb-loaded particles with moderate loading ratios demonstrated excellent deformability in microfluidic devices, easily deforming to pass through restricted pores half as wide as the diameter of the particles. The suspension of concentrated particles with a Hb concentration of 5.2 g/dL showed comparable viscosity to that of mouse blood, and the particles remained intact even after being sheared at a constant high rate (1000 1/s) for 10 min. Armed with the ability to control size, shape, deformability, and loading of Hb into RBC mimics, we will discuss the implications for artificial blood. PMID:22852860

  8. Bohr effect of hemoglobins: Accounting for differences in magnitude.

    Science.gov (United States)

    Okonjo, Kehinde O

    2015-09-01

    The basis of the difference in the Bohr effect of various hemoglobins has remained enigmatic for decades. Fourteen amino acid residues, identical in pairs and located at specific 'Bohr group positions' in human hemoglobin, are implicated in the Bohr effect. All 14 are present in mouse, 11 in dog, eight in pigeon and 13 in guinea pig hemoglobin. The Bohr data for human and mouse hemoglobin are identical: the 14 Bohr groups appear at identical positions in both molecules. The dog data are different from the human because three Bohr group positions are occupied by non-ionizable groups in dog hemoglobin; the pigeon data are vastly different from the human because six Bohr group positions are occupied by non-ionizable groups in pigeon hemoglobin. The guinea pig data are quite complex. Quantitative analyses showed that only the pigeon data could be fitted with the Wyman equation for the Bohr effect. We demonstrate that, apart from guinea pig hemoglobin, the difference between the Bohr effect of each of the other hemoglobins and of pigeon hemoglobin can be accounted for quantitatively on the basis of the occupation of some of their Bohr group positions by non-ionizable groups in pigeon hemoglobin. We attribute the anomalous guinea pig result to a new salt-bridge formed in its R2 quaternary structure between the terminal NH3(+) group of one β-chain and the COO(-) terminal group of the partner β-chain in the same molecule. The pKas of this NH3(+) group are 6.33 in the R2 and 4.59 in the T state.

  9. Learning to Baseline Business Technology

    Directory of Open Access Journals (Sweden)

    David Gore

    2013-10-01

    Full Text Available bills, sign multi-­‐year contracts, and make purchasing decisions without having an overall technology plan. That plan includes a technology baseline to fully assess existing technology. A CIO's goal is to align IT with business goals. Businesses must know total cost of ownership and the return on investment for all technology purchases and monthly costs. A business must also be able to manage technology assets and best utilize resources across the business. Teaching students to baseline technology will enable them to track and manage costs, discover errors and waste, and consolidate and improve existing technology.

  10. Hemoglobin of mice with radiation-induced mutations at the hemoglobin loci

    International Nuclear Information System (INIS)

    Chemical analyses were done on the abnormal hemoglobins of the five (101 x SEC)F1 offspring of X- irradiated adult SEC mice to determine which hemoglobin genes were expressed in each hemoglobin variant. Three offspring of irradiated SEC males did not express either of the two kinds of α-chains normally found in all SEC mice. The deficient α-chain synthesis caused these mice to exhibit an α-thalassemia similar to human α-thalassemia. Scanning electron microscopy was used to show that many erythrocytes of mice with α-thalassemia have bizarre shapes; e.g. many erythrocytes appeared flattened or had thorny projections (acanthocytes). One mutant with a tandem duplication of a segment of chromosome 7 (site of locus determining β-chain structure) produced twice as much SEC as 101 β-chain polypeptides. One mutant that probably arose by non-disjunction of chromosome 7's in its unirradiated 101 mother and loss of chromosome 7 from the gamete of its irradiated SEC father did not express the SEC β-chain gene. (author)

  11. Insights into Hemoglobin Assembly through in Vivo Mutagenesis of α-Hemoglobin Stabilizing Protein*

    Science.gov (United States)

    Khandros, Eugene; Mollan, Todd L.; Yu, Xiang; Wang, Xiaomei; Yao, Yu; D'Souza, Janine; Gell, David A.; Olson, John S.; Weiss, Mitchell J.

    2012-01-01

    α-Hemoglobin stabilizing protein (AHSP) is believed to facilitate adult Hemoglobin A assembly and protect against toxic free α-globin subunits. Recombinant AHSP binds multiple forms of free α-globin to stabilize their structures and inhibit precipitation. However, AHSP also stimulates autooxidation of αO2 subunit and its rapid conversion to a partially unfolded bishistidyl hemichrome structure. To investigate these biochemical properties, we altered the evolutionarily conserved AHSP proline 30 in recombinantly expressed proteins and introduced identical mutations into the endogenous murine Ahsp gene. In vitro, the P30W AHSP variant bound oxygenated α chains with 30-fold increased affinity. Both P30W and P30A mutant proteins also caused decreased rates of αO2 autooxidation as compared with wild-type AHSP. Despite these abnormalities, mice harboring P30A or P30W Ahsp mutations exhibited no detectable defects in erythropoiesis at steady state or during induced stresses. Further biochemical studies revealed that the AHSP P30A and P30W substitutions had minimal effects on AHSP interactions with ferric α subunits. Together, our findings indicate that the ability of AHSP to stabilize nascent α chain folding intermediates prior to hemin reduction and incorporation into adult Hemoglobin A is physiologically more important than AHSP interactions with ferrous αO2 subunits. PMID:22287545

  12. Insights into hemoglobin assembly through in vivo mutagenesis of α-hemoglobin stabilizing protein.

    Science.gov (United States)

    Khandros, Eugene; Mollan, Todd L; Yu, Xiang; Wang, Xiaomei; Yao, Yu; D'Souza, Janine; Gell, David A; Olson, John S; Weiss, Mitchell J

    2012-03-30

    α-Hemoglobin stabilizing protein (AHSP) is believed to facilitate adult Hemoglobin A assembly and protect against toxic free α-globin subunits. Recombinant AHSP binds multiple forms of free α-globin to stabilize their structures and inhibit precipitation. However, AHSP also stimulates autooxidation of αO(2) subunit and its rapid conversion to a partially unfolded bishistidyl hemichrome structure. To investigate these biochemical properties, we altered the evolutionarily conserved AHSP proline 30 in recombinantly expressed proteins and introduced identical mutations into the endogenous murine Ahsp gene. In vitro, the P30W AHSP variant bound oxygenated α chains with 30-fold increased affinity. Both P30W and P30A mutant proteins also caused decreased rates of αO(2) autooxidation as compared with wild-type AHSP. Despite these abnormalities, mice harboring P30A or P30W Ahsp mutations exhibited no detectable defects in erythropoiesis at steady state or during induced stresses. Further biochemical studies revealed that the AHSP P30A and P30W substitutions had minimal effects on AHSP interactions with ferric α subunits. Together, our findings indicate that the ability of AHSP to stabilize nascent α chain folding intermediates prior to hemin reduction and incorporation into adult Hemoglobin A is physiologically more important than AHSP interactions with ferrous αO(2) subunits.

  13. Increased hemoglobin O2 affinity protects during acute hypoxia.

    Science.gov (United States)

    Yalcin, Ozlem; Cabrales, Pedro

    2012-08-01

    Acclimatization to hypoxia requires time to complete the adaptation mechanisms that influence oxygen (O(2)) transport and O(2) utilization. Although decreasing hemoglobin (Hb) O(2) affinity would favor the release of O(2) to the tissues, increasing Hb O(2) affinity would augment arterial O(2) saturation during hypoxia. This study was designed to test the hypothesis that pharmacologically increasing the Hb O(2) affinity will augment O(2) transport during severe hypoxia (10 and 5% inspired O(2)) compared with normal Hb O(2) affinity. RBC Hb O(2) affinity was increased by infusion of 20 mg/kg of 5-hydroxymethyl-2-furfural (5HMF). Control animals received only the vehicle. The effects of increasing Hb O(2) affinity were studied in the hamster window chamber model, in terms of systemic and microvascular hemodynamics and partial pressures of O(2) (Po(2)). Pimonidazole binding to hypoxic areas of mice heart and brain was also studied. 5HMF decreased the Po(2) at which the Hb is 50% saturated with O(2) by 12.6 mmHg. During 10 and 5% O(2) hypoxia, 5HMF increased arterial blood O(2) saturation by 35 and 48% from the vehicle group, respectively. During 5% O(2) hypoxia, blood pressure and heart rate were 58 and 30% higher for 5HMF compared with the vehicle. In addition, 5HMF preserved microvascular blood flow, whereas blood flow decreased to 40% of baseline in the vehicle group. Consequently, perivascular Po(2) was three times higher in the 5HMF group compared with the control group at 5% O(2) hypoxia. 5HMF also reduced heart and brain hypoxic areas in mice. Therefore, increased Hb O(2) affinity resulted in hemodynamics and oxygenation benefits during severe hypoxia. This acute acclimatization process may have implications in survival during severe environmental hypoxia when logistic constraints prevent chronic acclimatization. PMID:22636677

  14. The relation of glycosylated hemoglobin level with coronary lesion in non-diabetic coronary heart disease individuals%非糖尿病冠心病患者糖化血红蛋白与冠脉病变相关性分析

    Institute of Scientific and Technical Information of China (English)

    鲁洁; 黄晓红

    2012-01-01

    Objective To examine the relation between HbA1C level and coronary lesion in non-diabetic coronary heart disease individuals. Methods 290 nondiabetic inpatients who diagnosised CHD by coronary angiography were enrolled with giycosylated hemoglobin level to low level group(n=115, HbA1C<5.6mmol/L),middle level group(n=91, HbA1C:5.7-5.9mmol/L),high level group(n=84, HbAlc>6.0mmol/L).The consequences of coronary angiography were judged by Gensini score. Results The Gensini score of high level group greater than middle level group , The Gensini score of middle level group greater than low level group, but did not receive principle of statistics significance. After adjusted the potential risk factors such as age, sex, smoking, drinking, the history of myocardial infarction, coronary artery lesion branch, fasting serum glucose, UA, TG, TC, HDL-C, LDL-C, HSCRP, BMI with Spearman partial correlation test, HbA1Clevel had no conspicuous relationship with Gensini score. Conclusions HbA1C has no obviously correlation with coronary stenosis degree in non—diabetic CHD individuals.%目的 探讨非糖尿病冠心病患者糖化血红蛋白(HbA1c)水平与冠状动脉病变的相关性.方法 选择经冠脉造影明确诊断冠心病的非糖尿病患者290例,按照HbA1c水平以三分位法分为低水平组(115例,HbA1c≤ 5.6mmol/L),中水平组(91例,HbA1c:5.7-5.9mol/L),高水平组(84例,HbA1c≥6.0mmol/L).冠状动脉造影结果使用Gensini评分.结果 HbA1c高水平组Gensini评分大于中水平组,中水平组Gensini评分大于低水平组,但无统计学意义.经Spearman偏等级相关分析,校正年龄、性别、吸烟状况、饮酒状况、心梗史、病变支数、空腹血糖、尿酸、甘油三酯、总胆固醇、高密度脂蛋白胆固醇、低密度脂蛋白胆固醇、高敏C反应蛋白、BMI(体重指数)等因素后,HbA1c水平与Gensini评分无显著关系.结论 在非糖尿病的冠心病人群中,HbA1c水平与冠脉狭窄程度无明显相关性.

  15. Genomic organization and evolution of the Atlantic salmon hemoglobin repertoire

    Directory of Open Access Journals (Sweden)

    Phillips Ruth B

    2010-10-01

    Full Text Available Abstract Background The genomes of salmonids are considered pseudo-tetraploid undergoing reversion to a stable diploid state. Given the genome duplication and extensive biological data available for salmonids, they are excellent model organisms for studying comparative genomics, evolutionary processes, fates of duplicated genes and the genetic and physiological processes associated with complex behavioral phenotypes. The evolution of the tetrapod hemoglobin genes is well studied; however, little is known about the genomic organization and evolution of teleost hemoglobin genes, particularly those of salmonids. The Atlantic salmon serves as a representative salmonid species for genomics studies. Given the well documented role of hemoglobin in adaptation to varied environmental conditions as well as its use as a model protein for evolutionary analyses, an understanding of the genomic structure and organization of the Atlantic salmon α and β hemoglobin genes is of great interest. Results We identified four bacterial artificial chromosomes (BACs comprising two hemoglobin gene clusters spanning the entire α and β hemoglobin gene repertoire of the Atlantic salmon genome. Their chromosomal locations were established using fluorescence in situ hybridization (FISH analysis and linkage mapping, demonstrating that the two clusters are located on separate chromosomes. The BACs were sequenced and assembled into scaffolds, which were annotated for putatively functional and pseudogenized hemoglobin-like genes. This revealed that the tail-to-tail organization and alternating pattern of the α and β hemoglobin genes are well conserved in both clusters, as well as that the Atlantic salmon genome houses substantially more hemoglobin genes, including non-Bohr β globin genes, than the genomes of other teleosts that have been sequenced. Conclusions We suggest that the most parsimonious evolutionary path leading to the present organization of the Atlantic salmon

  16. CASA Uno GPS orbit and baseline experiments

    Science.gov (United States)

    Schutz, B. E.; Ho, C. S.; Abusali, P. A. M.; Tapley, B. D.

    1990-01-01

    CASA Uno data from sites distributed in longitude from Australia to Europe have been used to determine orbits of the GPS satellites. The characteristics of the orbits determined from double difference phase have been evaluated through comparisons of two-week solutions with one-week solutions and by comparisons of predicted and estimated orbits. Evidence of unmodeled effects is demonstrated, particularly associated with the orbit planes that experience solar eclipse. The orbit accuracy has been assessed through the repeatability of unconstrained estimated baseline vectors ranging from 245 km to 5400 km. Both the baseline repeatability and the comparison with independent space geodetic methods give results at the level of 1-2 parts in 100,000,000. In addition, the Mojave/Owens Valley (245 km) and Kokee Park/Ft. Davis (5409 km) estimates agree with VLBI and SLR to better than 1 part in 100,000,000.

  17. Correlation of pretreatment hemoglobin and platelet counts with clinicopathological features in colorectal cancer in Saudi population

    Directory of Open Access Journals (Sweden)

    Eyad F Al-Saeed

    2014-01-01

    Full Text Available Background/Aims: In Saudi Arabia, colorectal cancers (CRCs are registered as the second most common cancers. However, no data has been reported about correlation of the severity of the anemia and pretreatment platelets level with clinicopathological features of CRCs. We aimed to evaluate the association between pretreatment hemoglobin and platelets level and the clinicopathological features of CRC patients in Saudi Arabia. Materials and Methods: Between September 2005 and November 2011, One hundred and fifty-four confirmed CRC patients underwent thorough physical examination, blood investigations, endoscopic ultrasonography (EUS, and computed tomography (CT for staging before surgery. Findings of physical assessment, EUS, CT, and pathological specimens were correlated with pretreatment hemoglobin and platelets levels the Pearson-Kendall tau correlative coefficients. Results: The mean age of cohort was 56.6 years (range: 26-89. Left-sided CRC were predominant (97 patients; 63%. Mean size of primary tumor was 6 cms (1-18 SD ± 3.55. Mean values of hemoglobin, red blood cells, hematocrit, white blood cells, and platelets were 11.9 SD ± 2.3, 35.5 SD ± 5.7, 4.43 × 10 6 /mL SD ± 0.6, 7.67 10 6 /mL SD ± 2.44, and 343 × 10 3 /mL SD ± 164.4, respectively. Pretreatment hemoglobin was inversely correlated with primary tumor size (R: 0.71, R2: 1.55, P = 0.0001 and nodal status (R: 0.02, R2: 0.05, P = 0.01. Right-sided CRC had significantly low pretreatment hemoglobin levels ( P = 0.001. Interestingly, pretreatment thrombocytosis was seen only in right-sided CRC (P = 0.0001. Conclusion: Pretreatment anemia and thrombocytosis were found mainly in right-sided CRCs and advanced primary and nodal stages. Pretreatment hemoglobin and thrombocytosis can be considered as useful prognostic markers in CRC patients.

  18. Up-regulation of alpha1-microglobulin by hemoglobin and reactive oxygen species in hepatoma and blood cell lines.

    Science.gov (United States)

    Olsson, Magnus G; Allhorn, Maria; Olofsson, Tor; Akerström, Bo

    2007-03-15

    alpha(1)-Microglobulin is a 26-kDa glycoprotein synthesized in the liver, secreted to the blood, and rapidly distributed to the extravascular compartment of all tissues. Recent results show that alpha(1)-microglobulin has heme-binding and heme-degrading properties and it has been suggested that the protein is involved in the defense against oxidation by heme and reactive oxygen species. In the present study the influence of hemoglobin and reactive oxygen species (ROS) on the cellular expression of alpha(1)-microglobulin was investigated. Oxy- and methemoglobin, free heme, and Fenton reaction-induced hydroxyl radicals induced a dose-dependent up-regulation of alpha(1)-microglobulin on both mRNA and protein levels in hepatoma cells and an increased secretion of alpha(1)-microglobulin. The up-regulation was reversed by the addition of catalase and ascorbate, and by reacting hemoglobin with cyanide which prevents redox reactions. Furthermore, the blood cell lines U937 and K562 expressed alpha(1)-microglobulin at low levels, and this expression increased up to 11-fold by the addition of hemoglobin. These results suggest that alpha(1)-microglobulin expression is induced by ROS, arising from redox reactions of hemoglobin or from other sources and are consistent with the hypothesis that alpha(1)-microglobulin participates in the defense against oxidation by hemoglobin, heme, and reactive oxygen species.

  19. Molecular characterization of hemoglobin from the honeybee Apis mellifera.

    Science.gov (United States)

    Hankeln, Thomas; Klawitter, Sabine; Krämer, Melanie; Burmester, Thorsten

    2006-07-01

    Due to the prevailing importance of the tracheal system for insect respiration, hemoglobins had been considered rare exceptions in this arthropod subphylum. Here we report the identification, cloning and expression analysis of a true hemoglobin gene in the honeybee Apis mellifera (Hymenoptera). The deduced amino acid sequence covers 171 residues (19.5kDa) and harbors all globin-typical features, including the proximal and the distal histidines. The protein has no signal peptide for transmembrane transport and was predicted to localize in the cytoplasm. The honeybee hemoglobin gene shows an ancient structure, with introns in positions B12.2 and G7.0, while most other insect globins have divergent intron positions. In situ hybridization studies showed that hemoglobin expression in the honeybee is mainly associated with the tracheal system. We also observe hemoglobin expression in the Malpighi tubes and testis. We further demonstrated that hemoglobins occur in other insect orders (Hemiptera, Coleoptera, Lepidoptera), suggesting that such genes belong to the standard repertoire of an insect genome. Phylogenetic analyses show that globins evolved along with the accepted insect systematics, with a remarkable diversification within the Diptera. Although insect hemoglobins may be in fact involved in oxygen metabolism, it remains uncertain whether they carry out a myoglobin-like function in oxygen storage and delivery. PMID:16698031

  20. Temperature-dependent enthalpy of oxygenation in Antarctic fish hemoglobins

    DEFF Research Database (Denmark)

    Fago, A.; Wells, R.M.G.; Weber, Roy E.

    1997-01-01

    The effect of temperature on the oxygen-binding properties of the hemoglobins of three cold-adapted Antarctic fish species, Dissostichus mawsoni, Pagothenia borchgrevinki and Trematomus, sp., has been investigated under different pH values and buffer conditions. A clear non linear van't Hoff plot...... literature data for the enthalpy of oxygenation in Antarctic fish hemoglobins derives from the use of the nonintegrated (linearized) form of the van't Hoff equation over different temperature ranges. The general assumption that a low heat of oxygenation in hemoglobins from polar animals represents...

  1. Preparation of porcine hemoglobin microcapsules of chitosan-sodium alginate

    Institute of Scientific and Technical Information of China (English)

    LI Jun; ZHANG Jijuan; ZHAO Xinjuan; YU Yan

    2007-01-01

    Using an emulsification-gelation method,chitosansodium alginate-porcine hemoglobin microcapsules were prepared.Results show that these microcapsules have better forms and small granules with 1 μm size of the mean particle size.They possess a relatively narrow and normal Gaussian distribution.The loading efficiency of porcine hemoglobin (pHb) in microcapsules is more than 90%.The pHb released from microcapsules is extended for more than one month.Chitosan-sodium alginate-hemoglobin microcapsules are expected to become an artificial oxygen-carrying therapeutic agent with sustained release for intravenous injection.

  2. Glycated hemoglobin and antidiabetic strategies as risk factors for hepatocellular carcinoma

    Institute of Scientific and Technical Information of China (English)

    Valter; Donadon; Massimiliano; Balbi; Francesca; Valent; Angelo; Avogaro

    2010-01-01

    AIM: To evaluate the relationship between glycemic control [assessed by glycated hemoglobin (HbA1c)], antidiabetic therapies and the risk of hepatocellular carcinoma (HCC).METHODS: We recruited 465 patients with HCC, 618 cases with liver cirrhosis and 490 controls with no liver disease. Among subjects with type 2 diabetes mellitus (DM2), the associations between the antidiabetic strategies and HbA1c level with HCC were determined through 2 series of multivariate logistic regression models using cirrhotic pa...

  3. Hemoglobin adducts of aromatic amines: associations with smoking status and type of tobacco.

    OpenAIRE

    Bryant, M S; Vineis, P; Skipper, P. L.; Tannenbaum, S R

    1988-01-01

    Hemoglobin adducts of 15 aromatic amines were determined in nonsmokers and smokers of blond- or black-tobacco cigarettes living in Turin, Italy. The subjects were all males age 55 or less and were representative of the population previously examined in a case/control study of bladder cancer. 4-Aminobiphenyl adduct levels were found to be significantly different in the three groups, and the differences were approximately proportional to the relative risk of each group. Adjustment for age and c...

  4. Independent association between glycated hemoglobin and arterial stiffness in healthy men

    OpenAIRE

    Noh, Jin‐Won; Kim, Eun‐Jung; Seo, Hyun‐Ju; Kim, Soo Geun

    2015-01-01

    Abstract Aims/Introduction Many studies have reported that high levels of glycated hemoglobin (HbA1c) are strongly associated with an increased risk of cardiovascular disease. Many researchers have not studied the association of HbA1c with various subclinical atherosclerosis phenotypes. We evaluated the impact of HbA1c on arterial stiffness and atherosclerosis in healthy Korean healthy men. Materials and Methods The study population included healthy adult men who participated in health check‐...

  5. Baseline budgeting for continuous improvement.

    Science.gov (United States)

    Kilty, G L

    1999-05-01

    This article is designed to introduce the techniques used to convert traditionally maintained department budgets to baseline budgets. This entails identifying key activities, evaluating for value-added, and implementing continuous improvement opportunities. Baseline Budgeting for Continuous Improvement was created as a result of a newly named company president's request to implement zero-based budgeting. The president was frustrated with the mind-set of the organization, namely, "Next year's budget should be 10 to 15 percent more than this year's spending." Zero-based budgeting was not the answer, but combining the principles of activity-based costing and the Just-in-Time philosophy of eliminating waste and continuous improvement did provide a solution to the problem.

  6. Glycosylated hemoglobin and mortality in patients with nondiabetic chronic kidney disease.

    Science.gov (United States)

    Menon, Vandana; Greene, Tom; Pereira, Arema A; Wang, Xuelei; Beck, Gerald J; Kusek, John W; Collins, Allan J; Levey, Andrew S; Sarnak, Mark J

    2005-11-01

    In the general population, hyperglycemia in the absence of diabetes may be associated with increased risk for mortality. Hyperglycemia is prevalent in chronic kidney disease; however, the relationship between glycosylated hemoglobin (HbA(1c)) as a marker of chronic hyperglycemia and outcomes has not been studied in nondiabetic chronic kidney disease. HbA(1c) was measured at baseline in the randomized cohort of the Modification of Diet in Renal Disease Study (n = 840). Participants with diabetes (n = 43), fasting glucose levels >126 mg/dl (n = 20), or missing HbA(1c) levels (n = 9) were excluded. Survival status until December 2000 was obtained from the National Death Index. Death was classified as cardiovascular (CVD) when the primary cause was International Classification of Disease, Ninth Revision codes 390 to 459. Cox models were performed to assess the relationship of HbA(1c) with all-cause and CVD mortality. Mean (SD) age was 52 (12) years, and mean (SD) GFR was 32 (12) ml/min per 1.73 m(2). Eighty-six percent of participants were white, and 61% were male. Mean (SD) HbA(1c) was 5.6% (0.5). A total of 169 (22%) patients died, 96 (13%) from CVD. After adjustment for randomization assignments and demographic, CVD, and kidney disease factors, HbA(1c) was a predictor of all-cause mortality (hazard ratio per 1% increase 1.73; 95% confidence interval 1.24 to 2.41; P = 0.001). There was a trend toward statistical significance in the relationship between HbA(1c) and CVD mortality (hazard ratio per 1% increase 1.53; 95% confidence interval 0.96 to 2.43; P = 0.07). HbA(1c) is associated with increased mortality in nondiabetic kidney disease. Hyperglycemia may be a potential therapeutic target and HbA(1c) may be important as a risk stratification tool in this high-risk population.

  7. Variant subunit specificity in the quaternary structure of Artemia hemoglobin.

    Science.gov (United States)

    Vandenberg, Cassandra J; Matthews, Charles M; Trotman, Clive N A

    2002-08-01

    The brine shrimp Artemia has three extracellular hemoglobins (Hbs) that are developmentally expressed and exhibit distinct oxygen-binding characteristics (Heip, Moens, and Kondo 1978; Heip et al. 1978 ). These Hbs are composed of two polymers, each of which comprises nine covalently linked globin domains. Although the cDNA sequences of two nine-domain globins from Artemia have been published, there is evidence for the existence of further expressed globin genes (Manning, Trotman, and Tate 1990 ). In the present study extensive analysis at the cDNA and genomic levels was performed in order to determine the globin gene copy number in Artemia. Sequence and Southern analysis suggest that four Hb polymers (T1, T2, C1, and C2) are expressed in Artemia. In addition, there is also at least one globin pseudogene. Protein sequencing of the native Hbs revealed that there are limitations on which two polymers can associate. The composition of the Hbs has been determined to be: Hb I, C1C2; Hb II, C1T2; and Hb III, T1T2. These pairings allow the levels of the three Artemia Hbs to be regulated independently by polymer expression alone, therefore explaining the previously inconsistent developmental and hypoxia-induced expression patterns. PMID:12140240

  8. Switch from epoetin to darbepoetin alfa in hemodialysis: dose equivalence and hemoglobin stability

    Directory of Open Access Journals (Sweden)

    Arrieta J

    2014-10-01

    Full Text Available Javier Arrieta,1 Iñigo Moina,1 José Molina,2 Isabel Gallardo,3 María Luisa Muñiz,4 Carmen Robledo,5 Oscar García,5 Fernando Vidaur,6 Rosa Inés Muñoz,3 Izaskun Iribar,7 Román Aguirre,7 Antonio Maza8 1Hospital de Basurto, Bilbao, 2Hospital de Donostia, Donostia-San Sebastián, 3Hospital de Galdakao-Usansolo, Galdakao, 4Hospital de Cruces, Baracaldo, 5Hospital de Santiago, Vitoria-Gasteiz, 6Policlínica de Guipúzcoa, Donostia-San Sebastián, 7Hemobesa Clinica Virgen Blanca, Bilbao, 8Dialbilbo, Bilbao, Spain Aim: The objective of the study reported here was to describe dose equivalence and hemoglobin (Hb stability in a cohort of unselected hemodialysis patients who were switched simultaneously from epoetin alfa to darbepoetin alfa. Methods: This was a multicenter, observational, retrospective study in patients aged $18 years who switched from intravenous (IV epoetin alfa to IV darbepoetin alfa in October 2007 (Month 0 and continued on hemodialysis for at least 24 months. The dose was adjusted to maintain Hb within 1.0 g/dL of baseline. Results: We included 125 patients (59.7% male, mean [standard deviation (SD] age 70.4 [13.4] years. No significant changes were observed in Hb levels (mean [SD] 11.9 [1.3] g/dL, 12.0 [1.5], 12.0 [1.5], and 12.0 [1.7] at Months −12, 0, 12 and 24, respectively, P=0.409. After conversion, the erythropoiesis-stimulating agent (ESA dose decreased significantly (P<0.0001, with an annual mean of 174.7 (88.7 international units (IU/kg/week for epoetin versus 95.7 (43.4 (first year and 91.4 (42.7 IU/kg/week (second year for darbepoetin (65% and 64% reduction, respectively. The ESA resistance index decreased from 15.1 (8.5 IU/kg/week/g/dL with epoetin to 8.1 (3.9 (first year and 7.9 (4.0 (second year with darbepoetin (P<0.0001. The conversion rate was 354:1 in patients requiring high (>200 IU/kg/week doses of epoetin and 291:1 in patients requiring low doses. Conclusion: In patients on hemodialysis receiving ESAs

  9. The Nuts and Bolts of Baseline Setting

    OpenAIRE

    Partnership for Market Readiness

    2012-01-01

    This document provides an overview of baseline setting for greenhouse gas (GHG) crediting mechanisms. The first section briefly explains the general purpose and objectives of setting a crediting mechanism baseline. The second section summarizes key policy considerations in defining and setting baselines. The final section covers important technical elements of baselines and provides an ove...

  10. Manipulation of hemoglobin expression affects Arabidopsis shoot organogenesis

    DEFF Research Database (Denmark)

    Wang, Yaping; Elhiti, Mohamed; Hebelstrup, Kim;

    2011-01-01

    Over the past few years non-symbiotic plant hemoglobins have been described in a variety of plant species where they fulfill several functions ranging from detoxification processes to basic aspects of plant growth and post-embryonic development. To date no information is available on the role...... of hemoglobins during invitro morphogenesis. Shoot organogenesis was induced in Arabidopsis lines constitutively expressing class 1, 2 and 3 hemoglobins (GLB1, 2 and 3) and lines in which the respective genes were either downregulated by RNAi (GLB1) or knocked out (GLB2 and GLB3). The process was executed......, 15, and 16), feed-back repressors of the cytokinin pathway, was repressed in both hemoglobin over-expressors whereas that of several Type-B ARRs (ARR2, 12, and 13), transcription activators of cytokinin-responsive genes, was induced. Such changes enhanced the sensitivity of the root explants...

  11. Receptor targeting of hemoglobin mediated by the haptoglobins

    DEFF Research Database (Denmark)

    Nielsen, Marianne Jensby; Moestrup, Søren Kragh

    2009-01-01

    Haptoglobin, the haptoglobin-hemoglobin receptor CD163, and the heme oxygenase-1 are proteins with a well-established function in the clearance and metabolism of "free" hemoglobin released during intravascular hemolysis. This scavenging system counteracts the potentially harmful oxidative and NO......-scavenging effects associated with "free" hemoglobin, and, furthermore, elicits an anti-inflammatory response. In the late primate evolution, haptoglobin variants with distinct functions have arisen, including haptoglobin polymers and the haptoglobin-related protein. The latter associates with a subspecies of high......-density lipoprotein (HDL) particles playing a crucial role in the innate immunity against certain trypanosome parasites. Recent studies have elucidated this fairly sophisticated immune defense mechanism that takes advantage of a trypanosomal haptoglobin-hemoglobin receptor evolved to supply the parasite with heme...

  12. Solid hemoglobin-polymer phantoms for evaluation of biophotonic systems.

    Science.gov (United States)

    Jang, Hyounguk; Pfefer, T Joshua; Chen, Yu

    2015-09-15

    Stable tissue phantoms that incorporate the spectral absorption properties of hemoglobin would benefit a wide range of biophotonic technologies. Toward this end, we have developed and validated a novel polymer material incorporating hemoglobin. Our solid hemoglobin-polymer (SHP) material is fabricated by mixing liquid silicone base with a hemoglobin solution, followed by sonication and low temperature curing. The optical properties of samples were determined over 450-1000 nm using the inverse adding-doubling method and the Beer-Lambert law. Measurements indicated SHP optical stability over four months. Near-infrared spectroscopy and hyperspectral imaging measurements of SHP samples were performed to demonstrate the utility of this approach. SHP materials have the potential to improve tissue-simulating phantoms used for development, evaluation, and standardization of optical devices for oximetry and other applications. PMID:26371926

  13. Reverse engineering the cooperative machinery of human hemoglobin.

    Science.gov (United States)

    Ren, Zhong

    2013-01-01

    Hemoglobin transports molecular oxygen from the lungs to all human tissues for cellular respiration. Its α2β2 tetrameric assembly undergoes cooperative binding and releasing of oxygen for superior efficiency and responsiveness. Over past decades, hundreds of hemoglobin structures were determined under a wide range of conditions for investigation of molecular mechanism of cooperativity. Based on a joint analysis of hemoglobin structures in the Protein Data Bank (Ren, companion article), here I present a reverse engineering approach to elucidate how two subunits within each dimer reciprocate identical motions that achieves intradimer cooperativity, how ligand-induced structural signals from two subunits are integrated to drive quaternary rotation, and how the structural environment at the oxygen binding sites alter their binding affinity. This mechanical model reveals the intricate design that achieves the cooperative mechanism and has previously been masked by inconsistent structural fluctuations. A number of competing theories on hemoglobin cooperativity and broader protein allostery are reconciled and unified.

  14. Reverse engineering the cooperative machinery of human hemoglobin.

    Directory of Open Access Journals (Sweden)

    Zhong Ren

    Full Text Available Hemoglobin transports molecular oxygen from the lungs to all human tissues for cellular respiration. Its α2β2 tetrameric assembly undergoes cooperative binding and releasing of oxygen for superior efficiency and responsiveness. Over past decades, hundreds of hemoglobin structures were determined under a wide range of conditions for investigation of molecular mechanism of cooperativity. Based on a joint analysis of hemoglobin structures in the Protein Data Bank (Ren, companion article, here I present a reverse engineering approach to elucidate how two subunits within each dimer reciprocate identical motions that achieves intradimer cooperativity, how ligand-induced structural signals from two subunits are integrated to drive quaternary rotation, and how the structural environment at the oxygen binding sites alter their binding affinity. This mechanical model reveals the intricate design that achieves the cooperative mechanism and has previously been masked by inconsistent structural fluctuations. A number of competing theories on hemoglobin cooperativity and broader protein allostery are reconciled and unified.

  15. 不同糖化血红蛋白控制水平糖尿病并发肺炎克雷伯杆菌肝脓肿患者的临床和影像学表现%Clinical and Imaging Features of Patients With Bacterial Liver Abscess Caused by Klebsiella Pneumoniae in Diabetics at Different Levels of Glycosylated Hemoglobin Control

    Institute of Scientific and Technical Information of China (English)

    刘波; 丁炎; 王爱花; 周群燕; 邬匡杰; 程海燕

    2016-01-01

    Objective To discuss the clinical and imaging features of patients with bacterial liver abscess caused by Klebsiella pneumoniae liver abscesses( KPLA)in diabetics at different levels of glycosylated hemoglobin( HbA1c )control. Methods A total of 146 patients with diabetes combined with KPLA admitted in Wuxi People′s Hospital of NanJing Medical University from January 2008 to September 2015 were selected. According to the levels of HbA1c ,they were divided into three groups,namely group A including 31 patients with good control of HbA1c level,HbA1c≤ 7. 0% ;group B including 52 patients with relatively control of HbA1c level,7. 0% 9. 0% . The basic information,underlying diseases,signs and symptoms,imaging features before abscess drainage,complications,abscess metastasis,treatment and prognosis of the patients in 3 groups were collected and compared. Results The time of hospital stay of patients in group B was longer than that in group A(P 9.0%。收集并比较3组患者基本资料、基础疾病、症状和体征、穿刺引流术前影像学表现、并发症、脓肿转移、治疗及预后情况。结果 B 组患者住院时间较 A 组延长(P <0.05);C 组患者年龄较 A 组和 B 组小、住院时间较 A 组和 B 组延长(P <0.05)。B 组患者高脂血症、慢性肾功能不全、超声检查胆管系统积气、CT 检查脓腔内积气及胸膜增厚、胸腔积液发生率较 A 组升高,C 组患者高脂血症、慢性肾功能不全、超声检查胆管系统积气、CT 检查脓腔内积气及胸膜增厚、胸腔积液、膈下脓肿发生率较 A 组和 B 组升高(P <0.01)。患者入院后均采用超声引导定位下肝脓肿穿刺引流,C 组患者 1例因全身多发感染并发多器官衰竭死亡,其余患者均恢复出院。结论糖尿病并发 KPLA 患者 HbA1c水平控制差者年龄偏小、住院时间较长,高脂血症、慢性肾功能不全发生率高,超声检查胆管系统积气、CT 检查脓

  16. 河北省沧州市成人糖化血红蛋白与血脂水平的流行病学调查%Epidemiological Investigation on Glycosylated Hemoglobin and Serum Lipid Levels in Adults in Cangzhou city of Hebei Province

    Institute of Scientific and Technical Information of China (English)

    赵连利; 刘可魂

    2014-01-01

    目的:对河北省沧州市成人糖化血红蛋白(glycosylated hemoglobin,HbAlc)、血糖、血脂水平进行流行病学调查,为该市心血管疾病等有关疾病的防治提供依据。方法:于2011年11月—2012年9月对河北省沧州市6287名20岁以上体检者测量身高、体质量、血压等,检测 HbAlc、空腹血糖(FPG)和血脂水平,并行肝脏彩超、胸部 X 线摄片、心电图等检查。结果:HbAlc 总异常率为16.7%,其中男性 HbAlc 异常率为18.8%,女性 HbAlc 异常率为14.0%,男性显著高于女性(P <0.01);血脂总异常率为43.0%,其中男性血脂异常率为54%,女性血脂异常率为27%,男性显著高于女性(P <0.01)。血脂异常以高三酰甘油(TG)、低高密度脂蛋白胆固醇(HDL-C)为主。结论:河北省沧州市成人 HbAlc、血脂异常率均高于全国平均水平,且男性高于女性,HbAlc、血脂异常与脂肪肝、动脉硬化等相关。%Objective:To perform epidemiological investigation on glycosylated hemoglobin(HbAlc),blood glucose and serum lipid levels in adults in Cangzhou city of Hebei province,so as to provide evidence for prevention of cardiac vascular diseases and other related diseases.Methods:Cross-sectional survey research was done from Nov.2011 to Sep.2012 to detect the levels of HbAlc,fasting plasma glucose(FPG)and serum lipid levels,as well as the liver color ultrasonography,chest X-ray,elec-trocardiogram,height,weight and blood pressure in 6287 persons over 20 years old,who undertook physical examination,in Cangzhou city of Hebei province.Results:Among all subjects,the incidence of abnormal HbAlc was 16.7%,of which male (18.8%)and was higher than female(14.0%)with significant difference(P <0.01).The incidence of dyslipidemia was 43. 0%,of which male(54%)was higher than female(27%)with significant difference(P <0.01).The incidences of high TG and low HDL-C were highlighted in

  17. Baseline scenarios of global environmental change

    International Nuclear Information System (INIS)

    This paper presents three baseline scenarios of no policy action computed by the IMAGE2 model. These scenarios cover a wide range of coupled global change indicators, including: energy demand and consumption; food demand, consumption, and production; changes in land cover including changes in extent of agricultural land and forest; emissions of greenhouse gases and ozone precursors; and climate change and its impacts on sea level rise, crop productivity and natural vegetation. Scenario information is available for the entire world with regional and grid scale detail, and covers from 1970 to 2100. (author)

  18. Low Modulus Biomimetic Microgel Particles with High Loading of Hemoglobin

    OpenAIRE

    CHEN, KAI; Merkel, Timothy J.; Pandya, Ashish; Napier, Mary E.; Luft, J. Christopher; Daniel, Will; Sheiko, Sergei; DeSimone, Joseph M.

    2012-01-01

    We synthesized extremely deformable red blood cell-like microgel particles and loaded them with bovine hemoglobin (Hb) to potentiate oxygen transport. With similar shape and size as red blood cells (RBCs), the particles were fabricated using the PRINT® (Particle Replication In Non-wetting Templates) technique. Low crosslinking of the hydrogel resulted in very low mesh density for these particles, allowing passive diffusion of hemoglobin throughout the particles. Hb was secured in the particle...

  19. Melanin and Hemoglobin Identification for Skin Disease Analysis

    OpenAIRE

    Liu, Zhao; Zerubia, Josiane

    2013-01-01

    International audience This paper proposes a novel method to extract melanin and hemoglobin concentrations of human skin, using bilateral decomposition with the knowledge of a multiple layered skin model and absorbance characteristics of major chromophores. Different from state-of-art approaches, the proposed method enables to address highlight and strong shading usually existing in skin color images captured under uncontrolled environment. The derived melanin and hemoglobin indices, direc...

  20. Free-radical production and oxidative reactions of hemoglobin.

    OpenAIRE

    Winterbourn, C C

    1985-01-01

    Mechanisms of autoxidation of hemoglobin, and its reactions with H2O2, O2-, and oxidizing or reducing xenobiotics are discussed. Reactive intermediates of such reactions can include drug free radicals, H2O2, and O2-, as well as peroxidatively active ferrylhemoglobin and methemoglobin-H2O2. The contributions of these species to hemoglobin denaturation and drug-induced hemolysis, and the actions of various protective agents, are considered.

  1. EPR studies of cooperative binding of Cu (II) to hemoglobin

    International Nuclear Information System (INIS)

    The investigation of the relative affinities of the two pairs of hemoglobin copper sites by monitoring the EPR spectra of the complexes formed by the reaction of copper with deoxyhemoglobin is reported. A model in which two sites are assumed to accept copper ions in a noncooperative way is not able to predict the experimental results. Thus it is conclude that the binding of these ions to hemoglobin is a cooperative phenomenon. (Author)

  2. An Effector of Hemoglobin Structure: The Guanosine 3', 5'-Triphosphate

    OpenAIRE

    Rezaei-Zarchi, Saeed; Imani, Saber; Soufian, Safieh; Panahi, Yunes; Saboury, Ali Akbar; Arefi, Mohammad Reza

    2012-01-01

    The effect of guanosine 3', 5'-triphosphate (GTP) on the hemoglobin structure was studied by UV-visible, fluorescence and circular dichroism (CD) spectroscopies, and cyclic voltammetry. UV-visible absorption spectra showed an increase in absorbance in the regions of 420 nm and 280 nm. Fluorescence spectra showed that the Trp fluorescence intensity increased upon excitation at 280 nm, when guanosine 3', 5'-triphosphate concentration was increased in hemoglobin solution. Along with the increase...

  3. Baseline Hematologic Results for Free-ranging White Rhinoceros (Ceratotherium simum) in Kruger National Park, South Africa.

    Science.gov (United States)

    Miller, Michele; Buss, Peter; Wanty, Rachel; Parsons, Sven; van Helden, Paul; Olea-Popelka, Francisco

    2015-10-01

    Complete blood counts (n = 115) and red blood cell analytes (n = 80) were assessed in free-ranging white rhinoceros (Ceratotherium simum) from Kruger National Park (KNP), South Africa. Blood was collected from healthy animals immobilized between February and September 2009-11 for management purposes. Our objectives were to establish baselines for KNP's white rhinoceros population and to compare results based on sex and age group. Significant differences in total white blood cells, total eosinophils, and hemoglobin were found between adult and subadult rhinoceros. Female rhinoceros had significantly higher total white blood cells and lower hemoglobin compared with males. Hematologic analytes were similar to those published for other rhinoceros populations, although the impact of capture and sampling methods, nutritional status, and habitat should be considered when comparing data. This baseline hematology for healthy free-ranging white rhinoceros in KNP may be useful in assessing health status for translocation and medical interventions, including treatment of poaching cases.

  4. β-珠蛋白生成障碍性贫血患者血红蛋白F表达与BCL11A基因rs11886868位点单核苷酸多态性的关系%Correlation Between Hemoglobin F Levels and Single Nucleotide Polymorphism at BCL11A Gene rs11886868 Locus in β-thalassemia Patients

    Institute of Scientific and Technical Information of China (English)

    陈群蓉; 孙顺昌; 彭运生; 王清; 莫宝妹

    2012-01-01

    本研究通过分析β-珠蛋白生成障碍性贫血患者的血红蛋白F(HbF)表达及BCL11A基因rs11886868位点的单核苷酸多态性,探讨二者之间的关系.选取89例已知基因突变类型的轻型β-珠蛋白生成障碍性贫血患者,通过毛细管电泳分析患者的红细胞HbF含量;提取患者基因组DNA,通过聚合酶链反应扩增含rs11886868位点的BCL11A基因片段,用DNA测序法确定rs11886868位点的单核苷酸多态性.结果显示,在89例深圳地区β-珠蛋白生成障碍性贫血患者的BCL11A基因rs11886868位点中发现C和T两种单核苷酸多态性;携带C/C单倍型患者的红细胞HbF含量为(4.47±3.42)%,高于携带C/T单倍型患者的(2.79±2.21)%.结论:β-珠蛋白生成障碍性贫血患者BCL11A基因rs11886868位点存在C和T两种单核苷酸多态性,其中C多态性可能与红细胞内HbF高表达存在相关性.%This study was aimed to analyze hemoglobin F (HbF) level and single nucleotide polymorphisms at rsl 1886868 locus of BCUIA gene in p-thalassemia patients, and to explore correlation between them. 89 mild β-thalassemia patients with known mutations were registered, and HbF levels were determined by capillary elcctrophoresis. Genomic DNA was extracted from peripheral leukocytes, fragment including rsll886868 locus in BCL11A gene was amplified by PCR, and polymorphism was determined by DNA sequencing. The results showed that 2 polymorphisms including C and T were found at rsl 1886868 locus in BCUIA gene among 89 mild β-thalassemia patients. HbF levels in red blood cells were (4. 47 ± 3. 42)% and (2. 79 ± 2. 21)% for β-thalassemia patients carrying C/C and C/T haplotypes, respectively. There was difference between 2 haplotype groups. It is concluded that the C and T polymorphisms are found at rsl 1886868 locus in the BCUIA gene for β-thalassemia patients. C polymorphism may be related to high HbF expression in red blood cells.

  5. Time course for the recovery of physical performance, blood hemoglobin, and ferritin content after blood donation

    DEFF Research Database (Denmark)

    Ziegler, Andreas K; Grand, Johannes; Stangerup, Ida;

    2015-01-01

    BACKGROUND: It is widely accepted that blood donation negatively affects endurance performance, but data on physical recovery after a standard blood donation are scarce. This study aimed to elucidate the temporary impact of blood donation on endurance performance, measured as peak oxygen uptake (VO......2peak ) and time trial (TT) performance. STUDY DESIGN AND METHODS: VO2peak , TT performance, blood, iron, and anthropometric variables were determined before (baseline) and 3, 7, 14, and 28 days after blood donation in 19 healthy men. RESULTS: VO2peak was reduced by 6.5% from 49.7 ± 2 mL/kg/min at...... baseline to 46.3 ± 2 mL/kg/min on Day 3 (p < 0.001), and TT performance was reduced by 5.2% from 13:31 ± 00:42 to 14:13 ± 00:50 min:sec (p < 0.001). Both VO2peak and TT performance were back to baseline 14 days after blood donation. Blood hemoglobin (Hb) concentration declined 7.9% from 9.3 ± 0.11 mmol...

  6. Value of Hemoglobin A2 Level Determined by Capillary Electrophoresis in Screening and Diagnosis of Thalassemia%毛细管电泳仪检测 HbA2在珠蛋白生成障碍性贫血筛查诊断中的价值

    Institute of Scientific and Technical Information of China (English)

    何英; 徐玉红; 张银辉; 杨来智; 陆学东

    2014-01-01

    Objective To evaluate the value of HbA2 level determined by capillary electrophoresis (Hb-CE)in screening and diagnosis of thalassemia.Methods HbA2 level of 249 thalassaemia carriers and 142 healthy controls confirmed by molecular biological detection were determined by Hb-CE method.The thalassaemia carrier subjects were divided into different groups and subgroups according to their results of gene detection.The sensitivity,specificity,accuracy,positive predictive value and negative predictive value for the diagnosis ofα-thalassemia,β-thalassaemia,α,β-thalassaemia were calculated under different HbA2 cut-off value.Results Mean value of HbA2 in healthy controls was (3.03±0.27)%.Mean values of HbA2 inα-thalassemia group and its subgroups of silentα-thalassemia,standardα-thalassemia and hemoglobin H disease were (2.38± 0.55)%,(2.61±0.46)%,(2.47 ± 0.32)% and (1.07 ± 0.17)%,respectively.Mean values of HbA2 inβ-thalassaemia group and itsβ0 subgroup,β+ subgroup were (5.65±0.47)%,(5.71±0.48)% and (5.56±0.43)%.Mean value of HbA2 in compoundαandβ-thalassaemia group was (5.7±0.82)%.Compared with healthy controls,HbA2 level inα-thalassemia group,silentα-thalassemia subgroup,standardα-thalassemia subgroup and hemoglobin H disease group decreased signifi-cantly (t values of 11.73,5.02,12.91 and 33.46,respectively,P0.05).HbA2 level inβ-thalassaemia group,β0 subgroup,β+ subgroup and compoundαandβ-thalassaemia group increased significantly (t values of 55.12,44.33,38.94 and 9.10,respectively,P0.05).Of 249 thalassemia carriers,all 124β-thalassaemia carriers were distinguished with ele-vated HbA2 level (>3.5%)determined by Hb-CE and only 57 were distinguished from 117α-thalassemia carriers by Hb-CE.Under the cut-off value of 2.5%,the sensitivity,specificity,positive predictive value,negative predictive value and accu-racy for the diagnosis ofα-thalassemia were 48.72%,97.18%,93.44%,69.70%,75.29%,respectively.Under the cut-off value of 3.5%,they were 100

  7. Induction of fetal hemoglobin through enhanced translation efficiency of γ-globin mRNA.

    Science.gov (United States)

    Hahn, Cynthia K; Lowrey, Christopher H

    2014-10-23

    Fetal hemoglobin (HbF) induction can ameliorate the clinical severity of sickle cell disease and β-thalassemia. We previously reported that activation of the eukaryotic initiation factor 2α (eIF2α) stress pathway increased HbF through a posttranscriptional mechanism. In this study, we explored the underlying means by which salubrinal, an activator of eIF2α signaling, enhances HbF production in primary human erythroid cells. Initial experiments eliminated changes in globin messenger RNA (mRNA) stability or cellular location and reduction of adult hemoglobin as possible salubrinal mechanisms. We then determined that salubrinal selectively increased the number of actively translating ribosomes on γ-globin mRNA. This enhanced translation efficiency occurred in the recovery phase of the stress response as phosphorylation of eIF2α and global protein synthesis returned toward baseline. These findings highlight γ-globin mRNA translation as a novel mechanism for regulating HbF production and as a pharmacologic target for induction of HbF. PMID:25170120

  8. Baseline LAW Glass Formulation Testing

    Energy Technology Data Exchange (ETDEWEB)

    Kruger, Albert A. [USDOE Office of River Protection, Richland, WA (United States); Mooers, Cavin [The Catholic University of America, Washington, DC (United States). Vitreous State Lab.; Bazemore, Gina [The Catholic University of America, Washington, DC (United States). Vitreous State Lab; Pegg, Ian L. [The Catholic University of America, Washington, DC (United States). Vitreous State Lab; Hight, Kenneth [The Catholic University of America, Washington, DC (United States). Vitreous State Lab; Lai, Shan Tao [The Catholic University of America, Washington, DC (United States). Vitreous State Lab; Buechele, Andrew [The Catholic University of America, Washington, DC (United States). Vitreous State Lab; Rielley, Elizabeth [The Catholic University of America, Washington, DC (United States). Vitreous State Lab; Gan, Hao [The Catholic University of America, Washington, DC (United States). Vitreous State Lab; Muller, Isabelle S. [The Catholic University of America, Washington, DC (United States). Vitreous State Lab; Cecil, Richard [The Catholic University of America, Washington, DC (United States). Vitreous State Lab

    2013-06-13

    The major objective of the baseline glass formulation work was to develop and select glass formulations that are compliant with contractual and processing requirements for each of the LAW waste streams. Other objectives of the work included preparation and characterization of glasses with respect to the properties of interest, optimization of sulfate loading in the glasses, evaluation of ability to achieve waste loading limits, testing to demonstrate compatibility of glass melts with melter materials of construction, development of glass formulations to support ILAW qualification activities, and identification of glass formulation issues with respect to contract specifications and processing requirements.

  9. Long-Baseline Neutrino Experiments

    CERN Document Server

    Diwan, M V; Qian, X; Rubbia, A

    2016-01-01

    We review long-baseline neutrino experiments in which neutrinos are detected after traversing macroscopic distances. Over such distances neutrinos have been found to oscillate among flavor states. Experiments with solar, atmospheric, reactor, and accelerator neutrinos have resulted in a coherent picture of neutrino masses and mixing of the three known flavor states. We will summarize the current best knowledge of neutrino parameters and phenomenology with our focus on the evolution of the experimental technique. We proceed from the first evidence produced by astrophysical neutrino sources to the current open questions and the goals of future research.

  10. Identification of a haptoglobin-hemoglobin complex in the Alaskan Least Cisco (Coregonus sardinella).

    Science.gov (United States)

    Wahl, S M; Boger, J K; Michael, V; Duffy, L K

    1992-01-01

    The hemoglobin and a hemoglobin binding protein have been characterized in the Arctic fish (Coregonus sardinella). The evolutionary significance of the hemoglobin and plasma protein differences between fish and mammals is still unresolved. Blood samples from the Alaskan Least Cisco were separated into plasma and hemoglobin fractions and the proteins in these fractions were analyzed both by alkaline agarose gel electrophoresis, by isolelectric focusing, and by capillary electrophoresis. Staining the plasma proteins gels with o-dianisidine revealed hemoglobin containing protein complexes. A hemoglobin-containing band was observed in hemolyzed plasma which did not migrate with free hemoglobin, and is believed to be hemoglobin-haptoglobin complex. Size exclusion chromatography further characterized the hemoglobin as disassociating freely into dimers, and hemoglobin-haptoglobin complex having a molecular weight greater then 200,000 daltons.

  11. Impaired CD163-mediated hemoglobin-scavenging and severe toxic symptoms in patients treated with gemtuzumab ozogamicin

    DEFF Research Database (Denmark)

    Maniecki, M.B.; Hasle, H.; Friis-Hansen, L.;

    2008-01-01

    , and low bilirubin after septicemia-induced intravascular hemolysis indicated abrogated clearance of haptoglobin-hemoglobin complexes. This was further supported by low levels of plasma soluble CD163 and a concordant low number of CD163-expressing monocytes. We show that CD163 positive monocytes...

  12. Urine sodium excretion after tolvaptan administration is dependent upon baseline serum sodium levels: a possible explanation for the improvement of hyponatremia with scarce chance of hypernatremia by a vasopressin receptor antagonist.

    Science.gov (United States)

    Imamura, Teruhiko; Kinugawa, Koichiro; Minatsuki, Shun; Muraoka, Hironori; Kato, Naoko; Inaba, Toshiro; Maki, Hisataka; Hatano, Masaru; Yao, Atsushi; Komuro, Issei

    2014-01-01

    Several studies have demonstrated that tolvaptan (TLV) can improve hyponatremia in advanced heart failure (HF) patients with rare chance of hypernatremia. However, changes in serum sodium concentrations (S-Na) in patients with or without hyponatremia during TLV treatment have not been analyzed.Ninety-seven in-hospital patients with decompensated HF who had received TLV at 3.75-15 mg/day for 1 week were enrolled. Among 68 "responders", who had achieved any increases in urine volume (UV) during the fi rst day, urinary sodium excretion during 24 hours (U-NaEx(24)) increased significantly during one week of TLV treatment along with higher baseline S-Na (P mEq/L; AUC, 0.711) for any increases in U-NaEx(24), we defined "hyponatremia" as S-Na mEq/L. In hyponatremic responders (n = 25), S-Na increased significantly, although 1 week was not sufficient for normalization (125.8 ± 5.0 versus 128.9 ± 4.3 mEq/L, P mEq/L, NS) along with increased U-NaEx(24) (2201 ± 1644 versus 4198 ± 3550 mg/day, P reduced ATP-supply in worse hemodynamics under hyponatremia. PMID:24632953

  13. The Relationship between Glycosylated Hemoglobin, Serum Visfatin Level and Perinatal Outcome in Pregnant Women with Normal Glucose Tolerance%糖耐量正常孕妇糖化血红蛋白、内脂素水平与围生结局的相关性研究

    Institute of Scientific and Technical Information of China (English)

    华绍芳; 匡德凤; 韩玉环

    2013-01-01

    Objective To investigate the relationship between the levels of serum glycosylated hemoglobin (HbA1c), serum visfatin, glucose and lipid metabolism and the perinatal outcome in pregnant women with normal glucose tolerance (NGT). Methods A total of 135 NGT pregnant women diagnosed by oral glucose tolerance test (OGTT) entered this study. Patients were divided into two groups, high HbA1c group (HbA1c≥5.5%, n=66) and normal HbA1c group (HbA1c<5.5%, n=69). The clinical data were collected in two groups. HbA1c levels were measured by HPLC, and the serum visfatin levels were measured by ELISA method. The values of body mass index (BMI), pregnant weight gain, visfatin, glucose and lipid met-abolic levels were analyzed between two groups. The relationship between levels of HbA1c, visfatin, glucose and lipid metab-olism and perinatal outcome were compared between two groups.Results The values of mean pregnancy weight gain, visfa-tin, total cholesterol (TC), triacylglycerol (TG), low density lipoprotein (LDL) and high density lipoprotein (HDL) were higher in high HbA1c group than those of normal HbA1c group. In NGT women with high HbA1c level,there were higher average birth weight, lower blood glucose level in newborn babies and higher rates of cesarean section. The incidences of polyhydram-nios, meconium-stained fluid, macrosomia and neonatal hypoglycemia were also higher in NGT women with high HbA 1c than those of normal HbA1c group. There was a positive relationship between HbA1c level, pregnant weight gain and visfatin and birth weight of newborn babies in 135 NGT pregnant women. Conclusion The excessive weight gain during pregnancy can induce the up-regulation of the visfatin level, resulting in the increased blood glucose level. The optimal timing and mode of delivery could prevent the occurrence of adverse pregnancy outcomes.%目的探讨糖耐量正常(NGT)孕妇血清糖化血红蛋白(HbA1c)、内脂素水平与糖脂代谢、围

  14. Relationship between glycated hemoglobin levels and adverse pregnancy outcomes in puerperas with gestational diabetes mellitus%妊娠期糖尿病患者糖化血红蛋白水平与不良妊娠结局的关系

    Institute of Scientific and Technical Information of China (English)

    王海霞

    2015-01-01

    目的:探究确诊妊娠期糖尿病的患者血清糖化血红蛋白(HbA1c)水平与不良妊娠结局的关系。方法以2013年11月~2014年12月中山市东凤人民医院(以下简称“我院”)妇产科收治的80例确诊妊娠期糖尿病的患者为研究组,选取我院同期非妊娠期糖尿病的正常孕妇80例为对照组,对研究组进行严格血糖控制,并对患者进行追踪研究至终止妊娠。检测研究组患者的HbA1c水平,比较不同HbA1c水平患者早产、羊水过多、巨大儿及胎儿宫内窘迫等发生情况。结果研究组早产、羊水过多、巨大儿及胎儿宫内窘迫发生率均高于对照组,差异均有统计学意义(P6.0%~6.5%、>6.5%~7.0%、>7.0%)而显著增加,不同HbA1c水平患者其不良妊娠结局的发生率比较,差异均有统计学意义(P0.05),且胎膜早破发生率随HbA1c水平升高无明显增加(P>0.05)。结论HbA1c是妊娠期糖尿病的重要临床评价指标,临床应加强HbA1c的检测以便更好地预判妊娠结局,确保母婴平安。%Objective To study the relationship between glycated hemoglobin (HbA1c) levels in gestational diabetes mel-litus (GDM) and obstetric adverse pregnancy outcomes. Methods 80 cases who were diagnosed with GDM in Depart-ment of Obstetrics and Gynecology of Dongfeng People's Hospital of Zhongshan City (“our hospital”for short) from Novem-ber 2013 to December 2014 were treated as study group, and another 80 pregnant women without GDM during the same period admitted in our hospital were chosen as control group. Study group was on glycemic control strictly, and then all cases were followed up until the termination of pregnancy. HbA1c of pregnant women in the study group were detected, the incidence of obstetric adverse symptoms (premature birth, polyhydramnios, fetal distress, macrosomia and premature rupture of membrane) of pregnant women with different levels of HbA1c was compared. Results The inci-dence of

  15. Comparison of Hemoglobin Transmition Spectrums of Healthy Persons and Patients with Schizophrenia Disorders

    OpenAIRE

    Vlokh R.; Marsel L.; Vlokh I.; Moroz O.; Dudok K.

    2002-01-01

    The present paper is devoted to the study of human hemoglobin transmission spectrums that were divided into four groups: hemoglobin of healthy persons, patients with schizophrenia, persons and people with and without the prodrome of schizophrenia that are in genetic susceptibility with patients. The change of absorption spectrums of hemoglobin of these groups was determined. The shifting of transmission minimums of the hemoglobin that correspond to electronic transitions in the hemoglobin mol...

  16. FED baseline engineering studies report

    Energy Technology Data Exchange (ETDEWEB)

    Sager, P.H.

    1983-04-01

    Studies were carried out on the FED Baseline to improve design definition, establish feasibility, and reduce cost. Emphasis was placed on cost reduction, but significant feasibility concerns existed in several areas, and better design definition was required to establish feasibility and provide a better basis for cost estimates. Design definition and feasibility studies included the development of a labyrinth shield ring concept to prevent radiation streaming between the torus spool and the TF coil cryostat. The labyrinth shield concept which was developed reduced radiation streaming sufficiently to permit contact maintenance of the inboard EF coils. Various concepts of preventing arcing between adjacent shield sectors were also explored. It was concluded that installation of copper straps with molybdenum thermal radiation shields would provide the most reliable means of preventing arcing. Other design studies included torus spool electrical/structural concepts, test module shielding, torus seismic response, poloidal conditions in the magnets, disruption characteristics, and eddy current effects. These additional studies had no significant impact on cost but did confirm the feasibility of the basic FED Baseline concept.

  17. FED baseline engineering studies report

    International Nuclear Information System (INIS)

    Studies were carried out on the FED Baseline to improve design definition, establish feasibility, and reduce cost. Emphasis was placed on cost reduction, but significant feasibility concerns existed in several areas, and better design definition was required to establish feasibility and provide a better basis for cost estimates. Design definition and feasibility studies included the development of a labyrinth shield ring concept to prevent radiation streaming between the torus spool and the TF coil cryostat. The labyrinth shield concept which was developed reduced radiation streaming sufficiently to permit contact maintenance of the inboard EF coils. Various concepts of preventing arcing between adjacent shield sectors were also explored. It was concluded that installation of copper straps with molybdenum thermal radiation shields would provide the most reliable means of preventing arcing. Other design studies included torus spool electrical/structural concepts, test module shielding, torus seismic response, poloidal conditions in the magnets, disruption characteristics, and eddy current effects. These additional studies had no significant impact on cost but did confirm the feasibility of the basic FED Baseline concept

  18. Uncertainty in Clean Development Mechanism Baselines: Sources, Ramifications, and Negotiations

    OpenAIRE

    Bushey, Douglas

    2005-01-01

    Awarding Certified Emission Reductions to Clean Development Mechanism (CDM) projects under the Kyoto Protocol involves comparing the actual emissions of the project to a counterfactual “baseline” emissions level. The baseline of a project is intended to represent the emissions that would have occurred in the absence of the project. Accordingly, there is a high degree of uncertainty in determining CDM baselines. In the years since the emergence of the CDM, scientific and political debates h...

  19. Baseline implementations of the Standard Line Editor (SLED)

    OpenAIRE

    Taylor, C F; Burnham, R.; Coulter, R.; Smart, S.; Cox, Lyle Ashton

    1980-01-01

    In response to a recognized requirement for a more uniform man machine interface, especially in multiple machine networks, a standardized text editor was proposed (1). This editor, "SLED" was designed to be easily implementable in several commonly available higher level languages. This document reviews two baseline implementations taken directly from the SLED standards which users may want to consider when implementing SLED upon local systems. These baseline programs were written and...

  20. Feasibility of Electrospinning the Globular Proteins Hemoglobin and Myoglobin

    Directory of Open Access Journals (Sweden)

    Catherine P. Barnes

    2006-11-01

    Full Text Available Various concentrations of the globular protein hemoglobin were successfully electrospun to create micro-fibrous mats of varying physical and mechanical characteristics. The electrospinning parameters are reported. One concentration of myoglobin was electrospun into a mat for comparison to the hemoglobin mats. Scanning electron microscopy revealed ribbon-like morphologies for the hemoglobin and myoglobin structures. Mean fiber width and thickness for each mat electrospun from a different hemoglobin concentration increased from 2.68 ± 0.83 to 3.55 ± 1.49 μm and from 0.49 ± 0.08 to 0.99 ± 0.41 μm, respectively, for increasing hemoglobin solution concentrations (from 150 to 225 mg/mL. For calculations of surface area to volume ratio for the four different electrospun hemoglobin concentrations, there was a negative correlation (r = -0.84 with concentration; the surface area to volume ratio ranged between 0.50 ± 0.16 and 1.53 ± 0.24 m2/cm3. Also, there appears to be a positive correlation between electrospun hemoglobin concentration and porosity, which increased with increasing concentration from 69.5 to 83.3 %. Following cross-linking with glutaraldehyde, the mechanical properties of two constructs were evaluated via uniaxial tensile testing to demonstrate handling capability. Results indicated that increased cross-linking time produced stiffer structures, as peak stress and modulus increased while strain at break decreased when the mats were cross-linked for 30 minutes with glutaraldehyde versus the 20 minute cross-linking time.

  1. Correlations between Levels of Low-Density Lipoprotein Cholesterol and Hemoglobin A1c and Severity of Coronary Artery Disease%低密度脂蛋白胆固醇和糖化血红蛋白水平与冠状动脉病变程度的相关性分析

    Institute of Scientific and Technical Information of China (English)

    王士强; 唐杨章; 张怀金; 吕留强; 赵立; 鲁晓

    2015-01-01

    Objective To study the correlations between the levels of low-density lipoprotein cholesterol (LDL-C)and hemoglobin A1c (HbA1c)and the severity of coronary artery disease. Methods According to the results of coronary angiography,238 patients with suspected coronary heart disease were divided into coronary heart disease (CHD)group (n = 162)and non-CHD group (n=76).Patients with CHD were subdivided into group A (LDL-C≤2.4 mmol·L-1 ,n=98)and group B (LDL-C>2.4 mmol·L-1 n=64).The levels of total cholesterol (TC),triacylg-lycerol (TG),LDL-C,high-density lipoprotein cholesterol (HDL-C)and HbA1c were measured in all patients.The severity of coronary artery disease was assessed using the Gensini score,and the correlations between TC,TG,HDL-C,LDL-C and HbA1c levels and Gensini score were ana-lyzed.Results Compared with non-CHD group,levels of TC,TG,LDL-C and HbA1c increased and levels of HDL-C decreased in CHD group (P 0.05). However,Gensini score was negatively correlated with HDL-C level (r=-0.168,P 0.05).Conclusion The levels of LDL-C and HbA1c are independently associated with the severity of coronary artery disease.Simultaneous increase in the levels of LDL-C and HbA1c is a significant predictor of the severity of coronary artery disease.%目的:探讨冠心病患者低密度脂蛋白胆固醇(LDL-C)、糖化血红蛋白(HbA1c)水平与冠状动脉病变程度的关系及其临床意义。方法将238例拟诊冠心病患者根据冠状动脉造影结果分为2组:冠心病组(162例)和非冠心病组(76例)。冠心病组又根据 LDL-C 水平分为2个亚组:A 组(LDL-C≤2.4 mmol·L-1,98例)和 B 组(LDL-C>2.4 mmol·L-1,64例)。冠心病组、非冠心病组分别测定总胆固醇(TC)、三酰甘油(TG)、LDL-C、高密度脂蛋白胆固醇(HDL-C)和 HbA1c 水平。对冠心病组冠状动脉病变程度进行 Gensini 评分,分析冠心病组 TC、TG、HDL-C、LDL-C、HbA1c 水平与 Gensini

  2. Antagonism by hemoglobin of effects induced by L-arginine in neuromuscular preparations from rats

    Directory of Open Access Journals (Sweden)

    C.R. Ambiel

    2001-04-01

    Full Text Available Nitric oxide (NO-synthase is present in diaphragm, phrenic nerve and vascular smooth muscle. It has been shown that the NO precursor L-arginine (L-Arg at the presynaptic level increases the amplitude of muscular contraction (AMC and induces tetanic fade when the muscle is indirectly stimulated at low and high frequencies, respectively. However, the precursor in muscle reduces AMC and maximal tetanic fade when the preparations are stimulated directly. In the present study the importance of NO synthesized in different tissues for the L-Arg-induced neuromuscular effects was investigated. Hemoglobin (50 nM did not produce any neuromuscular effect, but antagonized the increase in AMC and tetanic fade induced by L-Arg (9.4 mM in rat phrenic nerve-diaphragm preparations. D-Arg (9.4 mM did not produce any effect when preparations were stimulated indirectly at low or high frequency. Hemoglobin did not inhibit the decrease of AMC or the reduction in maximal tetanic tension induced by L-Arg in preparations previously paralyzed with d-tubocurarine and directly stimulated. Since only the presynaptic effects induced by L-Arg were antagonized by hemoglobin, the present results suggest that NO synthesized in muscle acts on nerve and skeletal muscle. Nevertheless, NO produced in nerve and vascular smooth muscle does not seem to act on skeletal muscle.

  3. Raman Tweezers as a Diagnostic Tool of Hemoglobin-Related Blood Disorders

    Directory of Open Access Journals (Sweden)

    Giulia Rusciano

    2008-12-01

    Full Text Available This review presents the development of a Raman Tweezers system for detecting hemoglobin-related blood disorders at a single cell level. The study demonstrates that the molecular fingerprint insight provided by Raman analysis holds great promise for distinguishing between healthy and diseased cells in the field of biomedicine. Herein a Raman Tweezers system has been applied to investigate the effects of thalassemia, a blood disease quite diffuse in the Mediterranean Sea region. By resonant excitation of hemoglobin Raman bands, we examined the oxygenation capability of normal, alpha- and beta-thalassemic erythrocytes. A reduction of this fundamental red blood cell function, particularly severe for beta-thalassemia, has been found. Raman spectroscopy was also used to draw hemoglobin distribution inside single erythrocytes; the results confirmed the characteristic anomaly (target shape, occurring in thalassemia and some other blood disorders. The success of resonance Raman spectroscopy for thalassemia detection reported in this review provide an interesting starting point to explore the application of a Raman Tweezers system in the analysis of several blood disorders.

  4. Pinellas Plant Environmental Baseline Report

    Energy Technology Data Exchange (ETDEWEB)

    1997-06-01

    The Pinellas Plant has been part of the Department of Energy`s (DOE) nuclear weapons complex since the plant opened in 1957. In March 1995, the DOE sold the Pinellas Plant to the Pinellas County Industry Council (PCIC). DOE has leased back a large portion of the plant site to facilitate transition to alternate use and safe shutdown. The current mission is to achieve a safe transition of the facility from defense production and prepare the site for alternative uses as a community resource for economic development. Toward that effort, the Pinellas Plant Environmental Baseline Report (EBR) discusses the current and past environmental conditions of the plant site. Information for the EBR is obtained from plant records. Historical process and chemical usage information for each area is reviewed during area characterizations.

  5. Integrated Baseline Review (IBR) Handbook

    Science.gov (United States)

    Fleming, Jon F.; Kehrer, Kristen C.

    2016-01-01

    The purpose of this handbook is intended to be a how-to guide to prepare for, conduct, and close-out an Integrated Baseline Review (IBR). It discusses the steps that should be considered, describes roles and responsibilities, tips for tailoring the IBR based on risk, cost, and need for management insight, and provides lessons learned from past IBRs. Appendices contain example documentation typically used in connection with an IBR. Note that these appendices are examples only, and should be tailored to meet the needs of individual projects and contracts. Following the guidance in this handbook will help customers and suppliers preparing for an IBR understand the expectations of the IBR, and ensure that the IBR meets the requirements for both in-house and contract efforts.

  6. 327 Building hazard baseline document

    International Nuclear Information System (INIS)

    This document identifies the hazards in the 327 Building at the time that a facility walk through was performed during FY99, presents a PHA of stabilization and deactivation activities, and provides a basis for the hazard evaluation and accident analysis that will be developed in the 327 Building Basis for Interim Operation (BIO). Activities addressed in this hazard baseline document include: (1) Stabilization and deactivation activities in preparation for eventual decommissioning of the 327 Building and the routine handling, processing, and shipment of waste to support these activities. (2) 324/327 Building Minimum Safe Project engineering and maintenance activities to maintain the building and systems viable--especially the Safety SSCs--to allow stabilization, deactivation, and waste handling activities with a minimum of risk to workers, the public, and the environment

  7. Perbedaan Kadar Hemoglobin dan Hematokrit Bayi Baru Lahir Akibat Perbedaan Waktu Penjepitan Tali Pusat

    Directory of Open Access Journals (Sweden)

    Nuriah Arma

    2016-01-01

    delayed 3 minute its can increase the volume red blood cells about 58%. The objective of this study was to prove the difference of hemoglobin and hematocrit level between 3 minutes and 1 minute cord clamping.  Experimental research was conducted with post-test only control group design of 36 newborns. Data were analyzed by t-test. The result of this study showed that the mean of hemoglobin and hematocrit level in 3 minutes of clamp are higher  than 1 minute of clamp.There was a significant difference of hemoglobin level between 3 minutes cord clamping group and 1 minute cord clamping group with p value is 0.004. There was also a significant difference of hematocrit level between 3 minutes cord clamping group and 1 minute cord clamping group with p value is 0.001. The conclusion is the hemoglobin and hematocrit level are better in cord clamping delayed in 3 minutes after birth than clamping in 1 minute after birth. Keywords: hemoglobin, hematocrit, cord clamping

  8. Atlantic NAD 83 SLA Baseline Tangents

    Data.gov (United States)

    Bureau of Ocean Energy Management, Department of the Interior — This data set contains baseline tangent lines in ArcGIS shapefile format for the BOEM Atlantic Region. Baseline tangent lines are typically bay or river closing...

  9. Atlantic NAD 83 SLA Baseline Points

    Data.gov (United States)

    Bureau of Ocean Energy Management, Department of the Interior — This data set contains baseline points in ArcGIS shapefile format for the BOEM Atlantic Region. Baseline points are the discrete coordinate points along the...

  10. Acetylator genotype-dependent formation of 2-aminofluorene-hemoglobin adducts in rapid and slow acetylator Syrian hamsters congenic at the NAT2 locus.

    Science.gov (United States)

    Feng, Y; Rustan, T D; Ferguson, R J; Doll, M A; Hein, D W

    1994-01-01

    Arylamine-hemoglobin adducts are a valuable dosimeter for assessing arylamine exposures and carcinogenic risk. The effects of age, sex, time-course, dose, and acetylator genotype on levels of 2-aminofluorene-hemoglobin adducts were investigated in homozygous rapid (Bio. 82.73/H-Patr) and slow (Bio. 82.73/H-Pats) acetylator hamsters congenic at the polymorphic (NAT2) acetylator locus. Following administration of a single ip dose of [3H]2-aminofluorene, peak 2-aminofluorene-hemoglobin adduct levels were achieved at 12-18 hr and retained a plateau up to 72 hr postinjection in both rapid and slow acetylator congenic hamsters. 2-Aminofluorene-hemoglobin adduct levels did not differ significantly between young (5-6 weeks) and old (32-49 weeks) hamsters or between male and female hamsters within either acetylator genotype. 2-Aminofluorene-hemoglobin adduct levels increased in a dose-dependent manner (r = 0.95, p = 0.0001) and were consistently higher in slow versus rapid acetylator congenic hamsters in studies of both time-course and dose-effect. The magnitude of the acetylator genotype-dependent difference was a function of dose; 2-aminofluorene-hemoglobin adduct levels were 1.5-fold higher in slow acetylator congenic hamsters following a 60 mg/kg 2-aminofluorene dose (p = 0.0013) but 2-fold higher following a 100 mg/kg 2-aminofluorene dose (p < 0.0001). These results show a specific and significant role for NAT2 acetylator genotype in formation of arylamine-hemoglobin adducts, which may reflect the relationship between acetylator genotype and the incidence of different cancers from arylamine exposures. PMID:8291051

  11. 糖化血红蛋白水平对糖尿病并发缺血性脑卒中患者再次脑血管事件的预测价值%Predictive Value of Glycosylated Hemoglobin Level on the Secondary Cerebrovascular Event of Diabetic Patients with Ischemic Stroke

    Institute of Scientific and Technical Information of China (English)

    孙强; 孙延鹏; 王云甫

    2014-01-01

    目的:探讨糖化血红蛋白水平(HbA1c)对糖尿病(DM)并发缺血性脑卒中(IS)患者再次脑血管事件(SCE)的预测作用。方法:连续收集2008年6月至2011年6月DM并发IS患者168例。入院后测定HbA1c水平,收集临床资料。依据HbA1c水平将患者分为HbA1c升高组(HbA1c≥6.1%)和HbA1c正常组(HbA1c<6.1%)。对所有患者出院后18个月内SCE进行随访(每2个月一次),依据是否发生SCE将患者分为事件组和非事件组。采用受试者工作特征(ROC)曲线评价HbA1c水平对SCE的预测价值。结果:与HbA1c正常组(n=75)相比,HbA1c升高组(n=93)入院时空腹血糖、2 h餐后血糖、收缩压、胆固醇、甘油三酯及低密度脂蛋白胆固醇水平均升高,高密度脂蛋白胆固醇水平和Barthel指数均明显降低(均<0.01)。事件组49例,HbA1c水平为(9.57±1.93)%;非事件组119例,HbA1c水平为(6.96±2.40)%,前者高于后者(<0.01)。ROC曲线下面积为0.784(<0.01,95%为0.713~0.855),取HbA1c水平6.55%为预测临界值的灵敏度和特异度分别为86.80%和64.30%。Kaplan-Meier生存曲线提示HbA1c水平≥6.55%时,SCE发生率增高(<0.01)。结论:DM合并IS患者首次入院时HbA1c水平可能对出院后18个月内SCE进行预测。%ObjectiveTo observe the predictive value of glycosylated hemoglobin (HbA1c) level on the secondary cerebrovascular events (SCE)of diabetic patients with ischemic stroke. Methods:From June 2008 to June 2011, 168 diabetic patients with ischemic stroke were admitted. The patients were divided into elevated HbA1c group (HbA1c≥6.1%) and normal HbA1c group (HbA1c<6.1%). All the patients have been followed up every two month and the occurrence of SCE was documented in 18 months. According to the follow-up data, the patients were assigned into the event and non-event groups. The power of HbA1c levels to predict SCE was exam-ined by receiver

  12. Impact of multi-micronutrient fortified rice on hemoglobin, iron and vitamin A status of Cambodian schoolchildren

    DEFF Research Database (Denmark)

    Perignon, Marlene; Fiorentino, Marion; Khov, Kuong;

    2016-01-01

    and transferrin receptors concentrations, and VA status, assessed using inflammation-adjusted retinol-binding protein concentration, were measured at the baseline, as well as at three and six months. Baseline prevalence of anemia, depleted iron stores, tissue iron deficiency, marginal VA status and VA deficiency...... were 15.6%, 1.4%, 51.0%, 7.9%, and 0.7%, respectively. The strongest risk factors for anemia were hemoglobinopathy, VA deficiency, and depleted iron stores (all p status, respectively, in comparison...... of schoolchildren. The impact on hemoglobin and iron status was limited, partly by sub-clinical inflammation. MMFR combined with non-nutritional approaches addressing anemia and inflammation should be further investigated....

  13. CASE REPORT: A RARE PRESENTATION OF HEMOGLOBIN D TRAIT

    Directory of Open Access Journals (Sweden)

    Mythri

    2013-12-01

    Full Text Available Hemoglobin D Punjab was first discovered in the early fifties in a mixed British and American family of Indian origin from the Los Angeles area; hence it is also sometimes called as “D Los Angeles” . Hemoglobin D is the 4th common hemoglobin variance . It developed as a response to the selective pressures of malaria in these regions . The sub stitution in Hb D is a glutamic acid to glutamine at the 121 st amino acid of the beta - globin chain (121Glu Gln . Hb D has an S - like mobility on alkaline electrophoresis but co - migrates with HbA on acid pH . Osmotic fragility may be decreased . Blood films m ay show target cells

  14. Bacillus anthracis secretes proteins that mediate heme acquisition from hemoglobin.

    Directory of Open Access Journals (Sweden)

    Anthony W Maresso

    Full Text Available Acquisition of iron is necessary for the replication of nearly all bacterial pathogens; however, iron of vertebrate hosts is mostly sequestered by heme and bound to hemoglobin within red blood cells. In Bacillus anthracis, the spore-forming agent of anthrax, the mechanisms of iron scavenging from hemoglobin are unknown. We report here that B. anthracis secretes IsdX1 and IsdX2, two NEAT domain proteins, to remove heme from hemoglobin, thereby retrieving iron for bacterial growth. Unlike other Gram-positive bacteria, which rely on cell wall anchored Isd proteins for heme scavenging, B. anthracis seems to have also evolved NEAT domain proteins in the extracellular milieu and in the bacterial envelope to provide for the passage of heme.

  15. A meta analysis of the correlation between baseline vitamin D level and sustained virological response of chronic hepatitis C%基线维生素D与慢性丙型肝炎持久病毒学应答相关性的Meta分析

    Institute of Scientific and Technical Information of China (English)

    张艳茹; 赖静兰; 张浩; 王俊洁; 齐明华; 于乐成; 周元平

    2014-01-01

    Objective To investigate the relationship between baseline vitamin D levels and sustained virological response (SVR) on the combination therapy of pegylated interferon plus ribavirin in patients with chronic infection of hepatitis C virus (HCV) genotype 1 or 4 .Methods Relevant studies were selected by systematically searching on PubMed ,Web of Science ,EMBASE ,the Cochrane Library and the Chinese Medical databases (CBM ,WanFang ,CNKI and Vip) .Studies referring to chronic hepatitis C (CHC) with HCV genotypes 1 or 4 ,treatment with pegylated interferon plus ribavirin ,and level of baseline vitamin D were collected .The differences of baseline vitamin D levels between SVR groups and non-SVR groups were compared and evaluated .Results Two hundred and ten references were searched out initially ,while only 5 were in accordance with the inclusion criterions for further analysis .Meta analysis was performed by software Stata 12 with random effect model .The results showed that the weighted mean difference (WMD) of baseline vitamin D level between SVR group (32 .9 ng/mL) and non-SVR group (28 .3 ng/mL) was 6 .759(95% CI:1 .786-11 .733) .The baseline vitamin D in SVR group was significantly higher than that in non-SVR group (Z=2 .66 ,P=0 .008) .Conclusion The baseline vitamin D level is positively correlated with SVR in CHC patients infected with HCV genotype 1 or 4 who undergo pegylated interferon plus ribavirin therapy .%目的:探讨基线维生素D水平与HCV基因1、4型感染者对标准抗病毒治疗持久病毒学应答(SVR)的关系。方法检索数据库中有关基线维生素D水平与HCV基因1、4型慢性感染者接受抗病毒治疗后SVR相关性的文献,meta分析比较SVR组和非SVR组的基线维生素D水平。结果共检索到210篇相关文献,其中符合纳入标准5篇,meta分析结果显示,基线维生素D水平在SVR组(32.9 ng/mL)与非SVR组(28.3 ng/mL)的加权均数差(WMD)为6.759(95% CI:1

  16. Relationship between hemoglobin A1c and cardiovascular disease in mild-to-moderate hypercholesterolemic Japanese individuals: subanalysis of a large-scale randomized controlled trial

    Directory of Open Access Journals (Sweden)

    Ohashi Yasuo

    2011-06-01

    Full Text Available Abstract Background Although the ADA/EASD/IDF International Expert Committee recommends using hemoglobin A1c (HbA1c to define diabetes, the relation between HbA1c and cardiovascular disease (CVD has not been thoroughly investigated. We analyzed this relation using clinical data on Japanese individuals with hypercholesterolemia. Methods In the large-scale MEGA Study 7832 patients aged 40 to 70 years old with mild-to-moderate hypercholesterolemia without CVD were randomized to diet alone or diet plus pravastatin and followed for >5 years. In the present subanalysis of that study a total of 4002 patients with baseline and follow-up HbA1c data were stratified according to having an average HbA1c during the first year of follow-up Results Overall, risk of CVD was significantly 2.4 times higher in individuals with HbA1c ≥6.5% versus Conclusions In hypercholesterolemic individuals the risk of CVD increases linearly with HbA1c level. This significant contribution by elevated HbA1c to increased CVD is independent of pravastatin therapy, and thus requires appropriate HbA1c management in addition to lipids reduction.

  17. Lead, hemoglobin, zinc protoporphyrin and ferritin concentrations in children

    Directory of Open Access Journals (Sweden)

    Rondó Patrícia H C

    2006-01-01

    Full Text Available OBJECTIVE: To assess the relationship of blood lead and hemoglobin, zinc protoporphyrin, and ferritin concentrations in children. METHODS: A cross-sectional study was carried out in 136 anemic and non-anemic children from two rural villages near a lead smelter in Adrianópolis, Southern Brazil, from July to September 2001. Hemoglobin electrophoresis was performed to exclude children with hemoglobin variants and thalassemia syndromes associated with anemia. Lead was determined by atomic absorption spectrophotometry; hemoglobin by automated cell counting; zinc protoporphyrin by hematofluorometry; ferritin by chemiluminescence. Student's t-test, Mann-Whitney test, and the c² test were used to assess the significance of the differences between the variables investigated in anemic and non-anemic children. Stepwise multivariate linear regression analysis was performed using two models for anemic and non-anemic children respectively. RESULTS: Lead was negatively associated to hemoglobin (p<0.017 in the first model, and in the second model lead was positively associated to zinc protoporphyrin (p<0.004 after controlling for ferritin, age, sex, and per capita income. There was an inverse association between hemoglobin and blood lead in anemic children. It was not possible to confirm if anemic children had iron deficiency anemia or subclinical infection, considering that the majority (90.4% had normal ferritin. CONCLUSIONS: The study detected a relationship between anemia and elevated blood lead concentrations. Further epidemiological studies are necessary to investigate the impact of iron nutritional interventions as an attempt to decrease blood lead in children.

  18. The Associations of High-Density Lipoprotein Subclasses With Insulin and Glucose Levels, Physical Activity, Resting Heart Rate, and Regional Adiposity in Men With Coronary Artery Disease: The Stanford Coronary Risk Intervention Project Baseline Survey

    OpenAIRE

    Williams, Paul T.; Haskell, William L; Vranizan, Karen M; Ronald M. Krauss

    1995-01-01

    We used nondenaturing polyacrylamide gradient gel electrophoresis to examine the associations of high-density lipoprotein (HDL) subclasses with adiposity, physical activity, resting heart rate (an indicator of sympathetic drive), and plasma insulin and glucose levels in 97 men with angiographically documented coronary artery disease. These men neither smoked nor used medications known to affect lipoproteins. The absorbency of protein stain was used as an index of mass concentrations at interv...

  19. Association of a complement receptor 1 gene variant with baseline erythrocyte sedimentation rate levels in patients starting anti-TNF therapy in a UK rheumatoid arthritis cohort: results from the Biologics in Rheumatoid Arthritis Genetics and Genomics Study Syndicate cohort

    OpenAIRE

    Bluett, J; I Ibrahim; Plant, D.; Hyrich, K L; Morgan, A.W.; A G Wilson; Isaacs, J.D.; [...; Gaston, H; Mulherin, D; Price, T; Sheeran, T; Chalam, V; S. Baskar; Emery, P

    2013-01-01

    Eligibility for anti-tumour necrosis factor (TNF) therapy in most European countries is restricted to severe, active rheumatoid arthritis (RA). The DAS28 score is a marker of disease severity and incorporates one of two inflammatory markers, erythrocyte sedimentation rate (ESR) or C-reactive protein. We aimed to determine the relation between genetic variants known to affect ESR and levels of ESR in patients with active RA. DNA samples were genotyped for four single-nucleotide polymorphisms (...

  20. Glycosylation of hemoglobin and plasma proteins in petrochemical plant workers

    Energy Technology Data Exchange (ETDEWEB)

    Unrug, A.; Tomaszewski, L.

    1985-01-01

    The concentration of glycosylated hemoglobin and (plasma) proteins has been measured in 111 workers of 6 MZRiP departments in Plock and in 54 healthy people. In all subjects the mean concentrations of glycosylated hemoglobin and glycosylated plasma proteins have been in so called wide range of normal values. Significant shifts of glycosylated Hb concentrations have been found in two departments--those of ethylenederivatives and distillation. The concentration of glycosylated plasma proteins has been elevated only in workers of the Catalytic Processes Department.

  1. Independency of Fe ions in hemoglobin on immunomagnetic reduction assay

    Energy Technology Data Exchange (ETDEWEB)

    Yang, S.Y. [MagQu Co. Ltd., Sindian City, Taipei County 231, Taiwan (China); Institute of Electro-optical Science and Technology, National Taiwan Normal University, Taipei 116, Taiwan (China); Lan, C.B.; Chen, C.H. [Institute of Electro-optical Science and Technology, National Taiwan Normal University, Taipei 116, Taiwan (China); Horng, H.E. [Institute of Electro-optical Science and Technology, National Taiwan Normal University, Taipei 116, Taiwan (China)], E-mail: phyfv001@scc.ntnu.edu.tw; Hong, Chin-Yih [Department of Mechanical Engineering, Nan-Kai University of Technology, Nantau County, Taiwan (China)], E-mail: cyhong@nkut.edu.tw; Yang, H.C. [Department of Physics, National Taiwan University, Taipei 106, Taiwan (China)], E-mail: hcyang@phys.ntu.edu.tw; Lai, Y.K. [College of Life Sciences, National Tsing Hua University, Hsinchu City 300, Taiwan (China); Department of Bioresources, Da-Yeh University, Changhua 515, Taiwan (China); Lin, Y.H.; Teng, K.S. [Apex Biotechnology Co. Ltd., Hsinchu City 300, Taiwan (China)

    2009-10-15

    Immunomagnetic reduction (IMR), which involves measuring the reduction in the ac magnetic susceptibility of magnetic reagents, is due to the association between bio-functionalized magnetic nanoparticles and target bio-molecules. This has been demonstrated for assaying proteins in solutions free of Fe ions, such as serum. In this work, the validity of IMR assay for samples rich in Fe ions like hemoglobin (Hb) is investigated. According to the results, there is no magnetic signal contributed by Fe-ion-rich Hb. Furthermore, the results show a high sensitivity in assaying hemoglobin A1c (HbA1c) by using IMR.

  2. Space Station-Baseline Configuration

    Science.gov (United States)

    1989-01-01

    In response to President Reagan's directive to NASA to develop a permanent marned Space Station within a decade, part of the State of the Union message to Congress on January 25, 1984, NASA and the Administration adopted a phased approach to Station development. This approach provided an initial capability at reduced costs, to be followed by an enhanced Space Station capability in the future. This illustration depicts the baseline configuration, which features a 110-meter-long horizontal boom with four pressurized modules attached in the middle. Located at each end are four photovoltaic arrays generating a total of 75-kW of power. Two attachment points for external payloads are provided along this boom. The four pressurized modules include the following: A laboratory and habitation module provided by the United States; two additional laboratories, one each provided by the European Space Agency (ESA) and Japan; and an ESA-provided Man-Tended Free Flyer, a pressurized module capable of operations both attached to and separate from the Space Station core. Canada was expected to provide the first increment of a Mobile Serving System.

  3. Shifting Baselines, Science, and Society

    Science.gov (United States)

    Jackson, J. B.

    2006-12-01

    All of us have a deeply personal concept of nature based upon our childhood perceptions of the world around us, and of the subsequent degradation of nature by the experiences of our lifetimes. Yet even the most rudimentary knowledge of history clearly demonstrates that the modern rise of human population and consumption have wreaked havoc on global ecosystems to the extent that nowhere is close to natural or pristine and that most places have been increasingly degraded over many centuries. This disconnect between direct personal experience and abstract historical perspective is the problem of "shifting baselines" that is the fundamental impediment to basic scientific understanding and environmental policy, and affects scientists as much as the general public, business, and government. Scientists in particular suffer from the inability to directly observe and experimentally verify causes and effects of previous changes in ecosystems that now bear so little resemblance to their natural state. Under the circumstances, it is essential for scientists to draw scientific conclusions based on imperfect data and to publicly explain, defend, and discuss their conclusions as the best possible science given present information. The failure to do so makes science virtually irrelevant to social and environmental policy and government.

  4. 2016 Annual Technology Baseline (ATB)

    Energy Technology Data Exchange (ETDEWEB)

    Cole, Wesley; Kurup, Parthiv; Hand, Maureen; Feldman, David; Sigrin, Benjamin; Lantz, Eric; Stehly, Tyler; Augustine, Chad; Turchi, Craig; O' Connor, Patrick; Waldoch, Connor

    2016-09-01

    Consistent cost and performance data for various electricity generation technologies can be difficult to find and may change frequently for certain technologies. With the Annual Technology Baseline (ATB), National Renewable Energy Laboratory provides an organized and centralized dataset that was reviewed by internal and external experts. It uses the best information from the Department of Energy laboratory's renewable energy analysts and Energy Information Administration information for conventional technologies. The ATB will be updated annually in order to provide an up-to-date repository of current and future cost and performance data. Going forward, we plan to revise and refine the values using best available information. The ATB includes both a presentation with notes (PDF) and an associated Excel Workbook. The ATB includes the following electricity generation technologies: land-based wind; offshore wind; utility-scale solar PV; concentrating solar power; geothermal power; hydropower plants (upgrades to existing facilities, powering non-powered dams, and new stream-reach development); conventional coal; coal with carbon capture and sequestration; integrated gasification combined cycle coal; natural gas combustion turbines; natural gas combined cycle; conventional biopower. Nuclear laboratory's renewable energy analysts and Energy Information Administration information for conventional technologies. The ATB will be updated annually in order to provide an up-to-date repository of current and future cost and performance data. Going forward, we plan to revise and refine the values using best available information.

  5. A free weekly iron-folic acid supplementation and regular deworming program is associated with improved hemoglobin and iron status indicators in Vietnamese women

    Directory of Open Access Journals (Sweden)

    Thach Tran D

    2009-07-01

    Full Text Available Abstract Background Anemia due to iron deficiency is recognized as one of the major nutritional deficiencies in women and children in developing countries. Daily iron supplementation for pregnant women is recommended in many countries although there are few reports of these programs working efficiently or effectively. Weekly iron-folic acid supplementation (WIFS and regular deworming treatment is recommended for non-pregnant women living in areas with high rates of anemia. Following a baseline survey to assess the prevalence of anemia, iron deficiency and soil transmitted helminth infections, we implemented a program to make WIFS and regular deworming treatment freely and universally available for all women of reproductive age in two districts of a province in northern Vietnam over a 12 month period. The impact of the program at the population level was assessed in terms of: i change in mean hemoglobin and iron status indicators, and ii change in the prevalence of anemia, iron deficiency and hookworm infections. Method Distribution of WIFS and deworming were integrated with routine health services and made available to 52,000 women. Demographic data and blood and stool samples were collected in baseline, and three and 12-month post-implementation surveys using a population-based, stratified multi-stage cluster sampling design. Results The mean Hb increased by 9.6 g/L (95% CI, 5.7, 13.5, p Conclusion A free, universal WIFS program with regular deworming was associated with reduced prevalence and severity of anemia, iron deficiency and hookworm infection when made available to Vietnamese women over a 12-month period.

  6. [Hemoglobins, XLVIII: the primary structure of hemoglobin of the Indian elephant (Elephas maximus, Proboscidea): beta 2 = Asn].

    Science.gov (United States)

    Braunitzer, G; Jelkmann, W; Stangl, A; Schrank, B; Krombach, C

    1982-07-01

    The primary structure of the hemoglobin of the Indian Elephant (Elephas maximus) is given. The sequence was determined automatically in a sequenator. By homologous comparison with adult human HbA, the alpha-chains differ by 24 exchanges and the beta-chains by 27 exchanges. Furthermore, we report p(O2)50 values with regard to altered contact sites with 2,3-bisphosphoglycerate in Indian elephant hemoglobin. Our findings explain the low p(O2)50 and the reduced interaction with 2,3-bisphosphoglycerate. Elephant hemoglobin has, like that of the Llama, only five phosphate binding sites. In addition, we have made an attempt to relate these results to aspects of respiratory physiology. Some implications of these biochemical and physiological results, concerning the Second Punic War and Hannibal's Alp transition, are given.

  7. Serum glycated albumin, but not glycated hemoglobin, is low in relation to glycemia in men with hypertriglyceridemia

    OpenAIRE

    Koga, Masafumi; Murai, Jun; SAITO, HIROSHI; Mukai, Mikio; Kasayama, Soji

    2010-01-01

    Abstract Aims/Introduction:  Serum glycated albumin (GA) and glycated hemoglobin (HbA1c) are influenced by plasma glucose levels, and are used for monitoring chronic glycemic control in diabetic patients. Both glycated proteins are known to be influenced by various factors other than plasma glucose levels. In the present study, we examined the effects of hypertriglyceridemia on them. Materials and Methods:  The present study comprised 273 non‐diabetic men. They were grouped into men with norm...

  8. Characterization of glycidol-hemoglobin adducts as biomarkers of exposure and in vivo dose

    Energy Technology Data Exchange (ETDEWEB)

    Honda, Hiroshi, E-mail: honda.hiroshi@kao.co.jp [R and D Safety Science Research, Kao Corporation, 2606 Akabane, Ichikai-Machi, Haga-Gun, Tochigi 321-3497 (Japan); Törnqvist, Margareta [Department of Materials and Environmental Chemistry, Environmental Chemistry Unit, Stockholm University, SE-106 91 Stockholm (Sweden); Nishiyama, Naohiro [R and D Safety Science Research, Kao Corporation, 2606 Akabane, Ichikai-Machi, Haga-Gun, Tochigi 321-3497 (Japan); Kasamatsu, Toshio, E-mail: kasamatsu.toshio@kao.co.jp [R and D Safety Science Research, Kao Corporation, 2606 Akabane, Ichikai-Machi, Haga-Gun, Tochigi 321-3497 (Japan)

    2014-03-15

    Hemoglobin adducts have been used as biomarkers of exposure to reactive chemicals. Glycidol, an animal carcinogen, has been reported to form N-(2,3-dihydroxy-propyl)valine adducts to hemoglobin (diHOPrVal). To support the use of these adducts as markers of glycidol exposure, we investigated the kinetics of diHOPrVal formation and its elimination in vitro and in vivo. Five groups of rats were orally administered a single dose of glycidol ranging from 0 to 75 mg/kg bw, and diHOPrVal levels were measured 24 h after administration. A dose-dependent increase in diHOPrVal levels was observed with high linearity (R{sup 2} = 0.943). Blood sampling at different time points (1, 10, 20, or 40 days) from four groups administered glycidol at 12 mg/kg bw suggested a linear decrease in diHOPrVal levels compatible with the normal turnover of rat erythrocytes (life span, 61 days), with the calculated first-order elimination rate constant (k{sub el}) indicating that the diHOPrVal adduct was chemically stable. Then, we measured the second-order rate constant (k{sub val}) for the reaction of glycidol with N-terminal valine in rat and human hemoglobin in in vitro experiments with whole blood. The k{sub val} was 6.7 ± 1.1 and 5.6 ± 1.3 (pmol/g globin per μMh) in rat and human blood, respectively, indicating no species differences. In vivo doses estimated from k{sub val} and diHOPrVal levels were in agreement with the area under the (concentration–time) curve values determined in our earlier toxicokinetic study in rats. Our results indicate that diHOPrVal is a useful biomarker for quantification of glycidol exposure and for risk assessment. - Highlight: • Glycidol-hemoglobin adduct (diHOPrVal) was characterized for exposure evaluation. • We studied the kinetics of diHOPrVal formation and elimination in vitro and in vivo. • Dose dependent formation and chemical stability were confirmed in the rat study. • In vivo dose (AUC) of glycidol could be estimated from diHOPrVal levels

  9. THE 2014 ALMA LONG BASELINE CAMPAIGN: AN OVERVIEW

    Energy Technology Data Exchange (ETDEWEB)

    Partnership, ALMA [Astrophysics Research Institute, Liverpool John Moores University, IC2, Liverpool Science Park, 146 Brownlow Hill, Liverpool L3 5RF (United Kingdom); Fomalont, E. B.; Vlahakis, C.; Corder, S.; Remijan, A.; Barkats, D.; Dent, W. R. F.; Phillips, N.; Cox, P.; Hales, A. S. [Joint ALMA Observatory, Alonso de Córdova 3107, Vitacura, Santiago (Chile); Lucas, R. [Institut de Planétologie et d’Astrophysique de Grenoble (UMR 5274), BP 53, F-38041 Grenoble Cedex 9 (France); Hunter, T. R.; Brogan, C. L.; Amestica, R.; Cotton, W. [National Radio Astronomy Observatory, 520 Edgemont Road, Charlottesville, VA 22903 (United States); Asaki, Y. [National Astronomical Observatory of Japan, 2-21-1 Osawa, Mitaka, Tokyo 181-8588 (Japan); Matsushita, S. [Institute of Astronomy and Astrophysics, Academia Sinica, P.O. Box 23-141, Taipei 106, Taiwan (China); Hills, R. E. [Astrophysics Group, Cavendish Laboratory, JJ Thomson Avenue, Cambridge CB3 0HE (United Kingdom); Richards, A. M. S. [Jodrell Bank Centre for Astrophysics, School of Physics and Astronomy, University of Manchester, Oxford Road, Manchester M13 9PL (United Kingdom); Broguiere, D., E-mail: efomalon@nrao.edu [Institut de Radioastronomie Millime´trique (IRAM), 300 rue de la Piscine, Domaine Universitaire, F-38406 Saint Martin d’Hères (France); and others

    2015-07-20

    A major goal of the Atacama Large Millimeter/submillimeter Array (ALMA) is to make accurate images with resolutions of tens of milliarcseconds, which at submillimeter (submm) wavelengths requires baselines up to ∼15 km. To develop and test this capability, a Long Baseline Campaign (LBC) was carried out from 2014 September to late November, culminating in end-to-end observations, calibrations, and imaging of selected Science Verification (SV) targets. This paper presents an overview of the campaign and its main results, including an investigation of the short-term coherence properties and systematic phase errors over the long baselines at the ALMA site, a summary of the SV targets and observations, and recommendations for science observing strategies at long baselines. Deep ALMA images of the quasar 3C 138 at 97 and 241 GHz are also compared to VLA 43 GHz results, demonstrating an agreement at a level of a few percent. As a result of the extensive program of LBC testing, the highly successful SV imaging at long baselines achieved angular resolutions as fine as 19 mas at ∼350 GHz. Observing with ALMA on baselines of up to 15 km is now possible, and opens up new parameter space for submm astronomy.

  10. Precise baseline determination for the TanDEM-X mission

    Science.gov (United States)

    Koenig, Rolf; Moon, Yongjin; Neumayer, Hans; Wermuth, Martin; Montenbruck, Oliver; Jäggi, Adrian

    The TanDEM-X mission will strive for generating a global precise Digital Elevation Model (DEM) by way of bi-static SAR in a close formation of the TerraSAR-X satellite, already launched on June 15, 2007, and the TanDEM-X satellite to be launched in May 2010. Both satellites carry the Tracking, Occultation and Ranging (TOR) payload supplied by the GFZ German Research Centre for Geosciences. The TOR consists of a high-precision dual-frequency GPS receiver, called Integrated GPS Occultation Receiver (IGOR), and a Laser retro-reflector (LRR) for precise orbit determination (POD) and atmospheric sounding. The IGOR is of vital importance for the TanDEM-X mission objectives as the millimeter level determination of the baseline or distance between the two spacecrafts is needed to derive meter level accurate DEMs. Within the TanDEM-X ground segment GFZ is responsible for the operational provision of precise baselines. For this GFZ uses two software chains, first its Earth Parameter and Orbit System (EPOS) software and second the BERNESE software, for backup purposes and quality control. In a concerted effort also the German Aerospace Center (DLR) generates precise baselines independently with a dedicated Kalman filter approach realized in its FRNS software. By the example of GRACE the generation of baselines with millimeter accuracy from on-board GPS data can be validated directly by way of comparing them to the intersatellite K-band range measurements. The K-band ranges are accurate down to the micrometer-level and therefore may be considered as truth. Both TanDEM-X baseline providers are able to generate GRACE baselines with sub-millimeter accuracy. By merging the independent baselines by GFZ and DLR, the accuracy can even be increased. The K-band validation however covers solely the along-track component as the K-band data measure just the distance between the two GRACE satellites. In addition they inhibit an un-known bias which must be modelled in the comparison, so the

  11. A Prospective Controlled Study of Kidney Donors: Baseline and 6-Month Follow-up

    Science.gov (United States)

    Kasiske, Bertram L.; Anderson-Haag, Teresa; Ibrahim, Hassan N.; Pesavento, Todd E.; Weir, Matthew R.; Nogueira, Joseph M.; Cosio, Fernando G.; Kraus, Edward S.; Rabb, Hamid H.; Kalil, Roberto S.; Posselt, Andrew A.; Kimmel, Paul L.; Steffes, Michael W.

    2013-01-01

    Background Most previous studies of living kidney donors have been retrospective and have lacked suitable healthy controls. Needed are prospective controlled studies to better understand the effects of a mild reduction in kidney function from kidney donation in otherwise normal individuals. Study Design Prospective, controlled, observational cohort study. Setting & Participants Consecutive patients approved for donation at 8 transplant centers in the US were asked to participate. For every donor enrolled, an equally healthy control with 2 kidneys who theoretically would have been suitable to donate a kidney was also enrolled. Predictor Kidney donation. Measurements At baseline pre-donation and at 6 months after donation, a medical history, vital signs, measured (iohexol) glomerular filtration rate and other measurements were collected. There were 201 donors and 198 controls that completed both baseline and 6 month visits and form the basis of this report. Results Compared to controls, donors had 28% lower glomerular filtration rate at 6 months (94.6±15.1 [SD] v. 67.6±10.1 mL/min/1.73m2; P<0.001), associated with a 23% greater parathyroid hormone (42.8±15.6 v. 52.7±20.9 pg/mL; P<0.001), 5.4% lower serum phosphate (3.5±0.5 v. 3.3±0.5 mg/dL; P<0.001), 3.7% lower hemoglobin (13.6±1.4 v. 13.1±1.2 g/dL; P<0.001), 8.2% greater uric acid (4.9±1.2 v. 5.3±1.1 mg/dL; P<0.001), 24% greater homocysteine (1.20±0.34 v. 1.49±0.43 mg/L; P<0.001), and 1.5% lower high density lipoprotein cholesterol (54.9±16.4 v. 54.1±13.9 mg/dL; P=0.03) level. There were no differences in albumin-creatinine ratios (5.0 [IQR, 4.0-6.6] v. 5.0 [IQR, 3.3-5.4] mg/g; P=0.5), office blood pressure, or glucose homeostasis. Limitations Short duration of follow-up and possible bias resulting from an inability to screen controls with kidney and vascular imaging performed in donors. Conclusions Kidney donors have some, but not all, abnormalities typically associated with mild chronic kidney

  12. Nonenzymatic glycosylation of human hemoglobin at multiple sites

    Energy Technology Data Exchange (ETDEWEB)

    Shapiro, R.; McManus, M.; Garrick, L.; McDonald, M.J.; Bunn, H.F.

    1979-04-01

    The most abundant minor hemoglobin component of human hemolysate is Hb A1c, which has glucose bound to the N-terminus of the beta chain by a ketoamine linkage. Hb A1c is formed slowly and continuously throughout the 120 day lifespan of the red cell. It can be synthesized in vitro by incubating purified hemoglobin with 14C-glucose. Other minor components, Hb A1a1 and Hb A1a2 are adducts of sugar phosphates at the N-terminus of the beta chain. Hb A1b contains an unidentified nonphosphorylated sugar at the beta N-terminus. In addition, a significant portion of the major hemoglobin component (Hb Ao) is also glycosylated by a glucose ketoamine linkage at other sites on the molecule, including the N-terminus of the alpha chain and the epsilon-amino group of several lysine residues on both the alpha and the beta chains. The results indicate that the interaction of glucose and hemoglobin is rather nonspecific and suggests that other proteins are modified in a similar fashion.

  13. Importance of hemoglobin concentration to exercise: acute manipulations

    DEFF Research Database (Denmark)

    Calbet, José A L; Lundby, Carsten; Koskolou, Maria;

    2006-01-01

    An acute reduction of blood hemoglobin concentration ([Hb]), even when the circulating blood volume is maintained, results in lower (.)V(O(2)(max) and endurance performance, due to the reduction of the oxygen carrying capacity of blood. Conversely, an increase of [Hb] is associated with enhanced...

  14. Hemoglobin, Growth, and Attention of Infants in Southern Ethiopia

    Science.gov (United States)

    Aubuchon-Endsley, Nicki L.; Grant, Stephanie L.; Berhanu, Getenesh; Thomas, David G.; Schrader, Sarah E.; Eldridge, Devon; Kennedy, Tay; Hambidge, Michael

    2011-01-01

    Male and female infants from rural Ethiopia were tested to investigate relations among hemoglobin (Hb), anthropometry, and attention. A longitudinal design was used to examine differences in attention performance from 6 (M = 24.9 weeks, n = 89) to 9 months of age (M = 40.6 weeks, n = 85), differences hypothesized to be related to changes in iron…

  15. Predictors of Glycated Hemoglobin among Jordanian Dia­betic Patients

    Directory of Open Access Journals (Sweden)

    Sawsan HAMMAD

    2015-11-01

    Full Text Available Background: We explored the level of Jordanian patients' knowledge, diabetes related distress, self-management activities and these effects on the A1C level.Methodology: This descriptive cross-sectional correlational design (conducted in 2013 was utilized to recruit 289 diabetic patients from outpatient diabetes clinics, using self-reported questionnaires (Diabetes Knowledge Test, Diabetes Distress Scale, and Diabetes Self-Management Questionnaire in addition to chart review for selected variables.Results: Participants' had mean glycated hemoglobin of 7.88%. Good glycemic control was significantly associated with higher self-management activities (r= -.147, high income (r= -.171, older age (r= -.252, shorter duration of illness (r= .153, and low levels of distress. Despite these relationships only age, duration of illness and income significantly predicted A1C (F (5, 284 = 11.57, P<.001, R2 = .17. Further, diabetes knowledge, diabetes-related distress, and self-management could not predict A1C level.Conclusion: Only diabetes-related distress and self-management correlated with patients' A1C, with no predictive power. Thus, further research is required to shed the light on the large unexplained components of the A1C variance. Keywords: Glycated hemoglobin, Diabetes related distress, Self-management, Diabetes 

  16. Glycosylated Hemoglobin Measurement in Dogs and Cats: Implications for its Utility in Diabetic Monitoring

    OpenAIRE

    Delack, J B; Stogdale, L.

    1983-01-01

    The measurement of glycosylated hemoglobin (HbA1) levels in humans is used to indicate the degree of long-term diabetic control. Using a commercially available kit for human HbA1, values were obtained for normal and diabetic dogs and cats. The normal range established in dogs was broad and overlapped considerably with the range in diabetics. Under the assay conditions and with a limited number of diabetic animals, the test was not found to be of value for dogs or cats.

  17. An assessment of the biotechnological use of hemoglobin modulation in cereals

    DEFF Research Database (Denmark)

    Hebelstrup, Kim; Shah, Jay K.; Simpson, Catherine;

    2014-01-01

    Non-symbiotic hemoglobin (nsHb) genes are ubiquitous in plants, but their biological functions have mostly been studied in model plant species rather than in crops. nsHb influences cell signaling and metabolism by modulating the levels of nitric oxide (NO). Class 1 nsHb is upregulated under hypox...... in cereal crops. These findings highlight the necessity for using actual crop plants rather than laboratory model plants when assessing the effects of biotechnological approaches to crop improvement. © 2013 Scandinavian Plant Physiology Society....

  18. Abnormal glycosylated hemoglobin as a predictive factor for glucose metabolism disorders in antipsychotic treatment

    Institute of Scientific and Technical Information of China (English)

    XU Leping; JI Juying; DUAN Yiyang; SHI Hui; ZHANG Bin; SHAO Yaqin; SUN Jian

    2007-01-01

    The aim of this study was to observe the changes in glucose metabolism after antipsychotic(APS)therapy,to note the influencing factors,as well as to dicuss the relationship between the occurrence of glucose metabolism disorders of APS origin and abnormal glycosylated hemoglobin(HbA1c)levels.One hundred and fifty-two patients with schizophrenia,whose fasting plasma glucose(FPG)and 2-h plasma glucose (2hPG)in the oral glucose tolerance test(2HPG)were normal,were grouped according to the HbA1c levels,one normal and the other abnormal,and were randomly enrolled into risperidone,clozapine and chlorpromazine treatment for six weeks.The FPG and 2hPG were measured at the baseline and at the end of the study.In the group with abnormal HbA1c and clozapine therapy,2HPG was higher after the study[(9.5±1.8)mmol/L]than that before the study[(7.2±1.4)mmol/L]and the difierence was statistically significant(P<0.01).FPG had no statistically significant difference before and after the study in any group(P>0.05).HbA1c levels and drugs contributing to 2HPG at the end of study had statistical cross-action(P<0.01).In the abnormal HbA1c group,2HPG after the study was higher in the clozapine treatment group [(9.5±1.8)mmol/L]than in the risperidone treatment group [(7.4±1.7)mmol/L]and the chlorpromazine treatment group[(7.3±1.6)mmol/L].The differences were statistically significant(P<0.01).In the normal HbA1c group there was no statistically significant difierence before and after the study in any group(P>0.05).2HPG before[(7.1±1.6)mmol/L]and after the study[(8.1±1.9)mmol/L]was higher in the abnormal HbA1c group than in the normal HbA1c group[(6.2±1.4)mmol/L vs(6.5±1.4)mmol/L]with the difierence being statistically significant(P<0.01 vs P<0.001).As compared with normal HbA1c group,the relative risk (RR)of glucose metabolism disease occurrence was 4.7 in the abnormal HDA1C group wlth the difierence being statistically significant(P<0.001).Patients with abnormal HbA1c

  19. Questa baseline and pre-mining ground-water quality investigation. 5. Well installation, water-level data, and surface- and ground-water geochemistry in the Straight Creek drainage basin, Red River Valley, New Mexico, 2001-03

    Science.gov (United States)

    Naus, Cheryl A.; McCleskey, R. Blaine; Nordstrom, D. Kirk; Donohoe, Lisa C.; Hunt, Andrew G.; Paillet, Frederick L.; Morin, Roger H.; Verplanck, Philip L.

    2005-01-01

    The U.S. Geological Survey, in cooperation with the New Mexico Environment Department, is investigating the pre-mining ground-water chemistry at the Molycorp molybdenum mine in the Red River Valley, northern New Mexico. The primary approach is to determine the processes controlling ground-water chemistry at an unmined, off-site, proximal analog. The Straight Creek drainage basin, chosen for this purpose, consists of the same quartz-sericite-pyrite altered andesitic and rhyolitic volcanic rock of Tertiary age as the mine site. The weathered and rugged volcanic bedrock surface is overlain by heterogeneous debris-flow deposits that interfinger with alluvial deposits near the confluence of Straight Creek and the Red River. Pyritized rock in the upper part of the drainage basin is the source of acid rock drainage (pH 2.8-3.3) that infiltrates debris-flow deposits containing acidic ground water (pH 3.0-4.0) and bedrock containing water of circumneutral pH values (5.6-7.7). Eleven observation wells were installed in the Straight Creek drainage basin. The wells were completed in debris-flow deposits, bedrock, and interfingering debris-flow and Red River alluvial deposits. Chemical analyses of ground water from these wells, combined with chemical analyses of surface water, water-level data, and lithologic and geophysical logs, provided information used to develop an understanding of the processes contributing to the chemistry of ground water in the Straight Creek drainage basin. Surface- and ground-water samples were routinely collected for determination of total major cations and selected trace metals; dissolved major cations, selected trace metals, and rare-earth elements; anions and alkalinity; and dissolved-iron species. Rare-earth elements were determined on selected samples only. Samples were collected for determination of dissolved organic carbon, mercury, sulfur isotopic composition (34S and 18O of sulfate), and water isotopic composition (2H and 18O) during

  20. Hospital doctors' assessment of baseline spirometry.

    OpenAIRE

    Stephenson, N.; Morgan, R.; Abdel-Rahman-Abdel-Wahab, E.; Turnbull, C. J.

    1998-01-01

    Baseline spirometry is useful in diagnosing and managing pulmonary disease. In a questionnaire survey of 100 hospital doctors in two hospitals in the Mersey region, their views and ability to interpret baseline spirometry was assessed. Of the 70 doctors who responded, 65% felt they could accurately interpret baseline spirometry. However, only 12% accurately interpreted all five vitalographs in the questionnaire. The majority (72%) felt they had not had adequate teaching in interpretation of s...

  1. Hazard Baseline Downgrade Effluent Treatment Facility

    International Nuclear Information System (INIS)

    This Hazard Baseline Downgrade reviews the Effluent Treatment Facility, in accordance with Department of Energy Order 5480.23, WSRC11Q Facility Safety Document Manual, DOE-STD-1027-92, and DOE-EM-STD-5502-94. It provides a baseline grouping based on the chemical and radiological hazards associated with the facility. The Determination of the baseline grouping for ETF will aid in establishing the appropriate set of standards for the facility

  2. Effect of repeated benzene inhalation exposures on benzene metabolism, binding to hemoglobin, and induction of micronuclei.

    Science.gov (United States)

    Sabourin, P J; Sun, J D; MacGregor, J T; Wehr, C M; Birnbaum, L S; Lucier, G; Henderson, R F

    1990-05-01

    Metabolism of benzene is thought to be necessary to produce the toxic effects, including carcinogenicity, associated with benzene exposure. To extrapolate from the results of rodent studies to potential health risks in man, one must know how benzene metabolism is affected by species, dose, dose rate, and repeated versus single exposures. The purpose of our studies was to determine the effect of repeated inhalation exposures on the metabolism of [14C]benzene by rodents. Benzene metabolism was assessed by characterizing and quantitating urinary metabolites, and by quantitating 14C bound to hemoglobin and micronuclei induction. F344/N rats and B6C3F1 mice were exposed, nose-only, to 600 ppm benzene or to air (control) for 6 hr/day, 5 days/week for 3 weeks. On the last day, both benzene-pretreated and control animals were exposed to 600 ppm, 14C-labeled benzene for 6 hr. Individual benzene metabolites in urine collected for 24 hr after the exposure were analyzed. There was a significant decrease in the respiratory rate of mice (but not rats) pretreated with benzene which resulted in lower levels of urinary [14C]benzene metabolites. The analyses indicated that the only effects of benzene pretreatment on the metabolite profile in rat or mouse urine were a slight shift from glucuronidation to sulfation in mice and a shift from sulfation to glucuronidation in rats. Benzene pretreatment also had no effect, in either species, on formation of [14C]benzene-derived hemoglobin adducts. Mice and rats had similar levels of hemoglobin adduct binding, despite the higher metabolism of benzene by mice. This indicates that hemoglobin adduct formation occurs with higher efficiency in rats. After 1 week of exposure to 600 ppm benzene, the frequency of micronucleated, polychromatic erythrocytes (PCEs) in mice was significantly increased. Exposure to the same level of benzene for an additional 2 weeks did not further increase the frequency of micronuclei in PCEs. These results indicate

  3. Comparable application of the OCT and Abbe refractometers for measurements of glycated hemoglobin portion in blood

    Science.gov (United States)

    Zhernovaya, Olga S.; Tuchin, Valery V.; Wang, Ruikang K.

    2006-02-01

    It is known that glucose interacts with plasma proteins and hemoglobin in erythrocytes. Glycated (glycosylated) hemoglobin is the result of an irreversible non-enzymatic fixation of glucose on the beta chain of hemoglobin A. The amount of glycated hemoglobin depends on blood glucose concentration and reflects the mean glycemia of about the previous 2-3 months. Glycated hemoglobin is a useful marker for long-term glucose control in diabetic patients. Therefore, the search of quick and high sensitive methods for measurement of glycated hemoglobin portion in blood is important. This study is focused on the determination of refractive index of hemoglobin solution at different glucose concentrations. Measurements were performed using Abbe refractometer at 589 nm and optical coherence tomography (OCT) at 820 nm. The different amount of glucose (from 0 to 1000 mg/dl with a step 100 mg/dl) was added to hemoglobin solution. Theoretical values of refractive index of hemoglobin solutions with glucose were calculated supposing non-interacting hemoglobin and glucose molecules. There is a difference between measured and calculated values of refractive index. This difference is due to glucose binding to hemoglobin. It is shown that the refractive index measurements can be applied for the evaluation of glycated hemoglobin amount.

  4. Megalin and cubilin are endocytic receptors involved in renal clearance of hemoglobin

    DEFF Research Database (Denmark)

    Gburek, Jakub; Verroust, Pierre J; Willnow, Thomas E;

    2002-01-01

    The kidney is the main site of hemoglobin clearance and degradation in conditions of severe hemolysis. Herein it is reported that megalin and cubilin, two epithelial endocytic receptors, mediate the uptake of hemoglobin in renal proximal tubules. Both receptors were purified by use of hemoglobin-...

  5. 基线hs-CRP和LDL-C水平与不同急性冠状动脉综合征急性期预后的相关性及意义%Correlation and significance of baseline hs-CRP and LDL-C levels with acute coronary syndrome prognosis

    Institute of Scientific and Technical Information of China (English)

    曹春辉; 黄军; 王仲华; 方永祥; 张晓红

    2012-01-01

    目的 探讨基线高敏C-反应蛋白(hs-CRP)和低密度脂蛋白胆固醇(LDL-C)水平与急性冠状动脉综合征(ACS)急性期预后的相关性及意义.方法 选取某院98例冠状动脉综合征患者,所有患者入院24h内测定基线状态的hs-CRP和LDL-C水平,治疗30 d后,按出现死亡、事件和无事件将患者分成3组,将3组患者基线hs-CRP和LDL-C水平进行比较分析,并对死亡组和事件组包括性别、年龄、hs-CRP、LDL-C在内的多项危险因素进行多因素Logistic回归分析,判断各因素对死亡率和事件发生率的影响.结果 治疗30 d后,死亡组的基线hs-CRP水平(61.3±35.1)显著高于事件组(35.2±17.3)和无事件组(11.9±5.2),P<0.05;死亡组的基线LDL-C水平(3.5±1.2)与事件组(3.4±2.1)和无事件组(3.1±1.8),差异无统计学意义,P>0.05;Logistic回归分析发现,基线hs-CRP水平(OR=3.126)、ST异常抬高(OR=2.831)、LVEF(OR=2.143)、Cr水平(OR=2.809)、糖尿病(OR=3.219)等是影响死亡的危险因素,P<0.05;高血压(OR=2.723)、中风史(OR=2.185)、吸烟(OR=2.139)、基线hs-CRP水平(OR=2.536)、ST异常抬高(OR=2.941)、LVEF(OR=2.735)、Cr水平(OR=3.023)是影响血管事件的危险因素,P<0.05;事件组和无事件组的hs-CRP水平较治疗前均显著降低,P<0.05;两组LDL-C水平治疗后较治疗前均略有降低,差异无统计学意义,P>0.05.结论 ACS患者急性期死亡和事件与hs-CRP水平明显相关,但与基线LDL-C水平无关.治疗后Hs-CRP水平明显降低,可作为急性期治疗的靶目标.%OBJECTIVE To investigate the correlation and significance for baseline high-sensitivity C-reactive protein (hs-CRP) and low-density lipoprotein cholesterol ( LDL-C) levels in the prognosis of patients with acute coronary syndrome (ACS). METHODS 98 cases of coronary syndrome patients in our hospital were chosen. The level of baseline hs-CRP and LDL-C of all patients in our hospital within 24 hours was measured. The determination of

  6. Evaluation of non cyanide methods for hemoglobin estimation

    Directory of Open Access Journals (Sweden)

    Vinaya B Shah

    2011-01-01

    Full Text Available Background: The hemoglobincyanide method (HiCN method for measuring hemoglobin is used extensively worldwide; its advantages are the ready availability of a stable and internationally accepted reference standard calibrator. However, its use may create a problem, as the waste disposal of large volumes of reagent containing cyanide constitutes a potential toxic hazard. Aims and Objective: As an alternative to drabkin`s method of Hb estimation, we attempted to estimate hemoglobin by other non-cyanide methods: alkaline hematin detergent (AHD-575 using Triton X-100 as lyser and alkaline- borax method using quarternary ammonium detergents as lyser. Materials and Methods: The hemoglobin (Hb results on 200 samples of varying Hb concentrations obtained by these two cyanide free methods were compared with a cyanmethemoglobin method on a colorimeter which is light emitting diode (LED based. Hemoglobin was also estimated in one hundred blood donors and 25 blood samples of infants and compared by these methods. Statistical analysis used was Pearson`s correlation coefficient. Results: The response of the non cyanide method is linear for serially diluted blood samples over the Hb concentration range from 3gm/dl -20 gm/dl. The non cyanide methods has a precision of + 0.25g/dl (coefficient of variation= (2.34% and is suitable for use with fixed wavelength or with colorimeters at wavelength- 530 nm and 580 nm. Correlation of these two methods was excellent (r=0.98. The evaluation has shown it to be as reliable and reproducible as HiCN for measuring hemoglobin at all concentrations. The reagents used in non cyanide methods are non-biohazardous and did not affect the reliability of data determination and also the cost was less than HiCN method. Conclusions: Thus, non cyanide methods of Hb estimation offer possibility of safe and quality Hb estimation and should prove useful for routine laboratory use. Non cyanide methods is easily incorporated in hemobloginometers

  7. Characterization and Expression of the Lucina pectinata Oxygen and Sulfide Binding Hemoglobin Genes.

    Directory of Open Access Journals (Sweden)

    Ingrid M Montes-Rodríguez

    Full Text Available The clam Lucina pectinata lives in sulfide-rich muds and houses intracellular symbiotic bacteria that need to be supplied with hydrogen sulfide and oxygen. This clam possesses three hemoglobins: hemoglobin I (HbI, a sulfide-reactive protein, and hemoglobin II (HbII and III (HbIII, which are oxygen-reactive. We characterized the complete gene sequence and promoter regions for the oxygen reactive hemoglobins and the partial structure and promoters of the HbI gene from Lucina pectinata. We show that HbI has two mRNA variants, where the 5'end had either a sequence of 96 bp (long variant or 37 bp (short variant. The gene structure of the oxygen reactive Hbs is defined by having 4-exons/3-introns with conservation of intron location at B12.2 and G7.0 and the presence of pre-coding introns, while the partial gene structure of HbI has the same intron conservation but appears to have a 5-exon/ 4-intron structure. A search for putative transcription factor binding sites (TFBSs was done with the promoters for HbII, HbIII, HbI short and HbI long. The HbII, HbIII and HbI long promoters showed similar predicted TFBSs. We also characterized MITE-like elements in the HbI and HbII gene promoters and intronic regions that are similar to sequences found in other mollusk genomes. The gene expression levels of the clam Hbs, from sulfide-rich and sulfide-poor environments showed a significant decrease of expression in the symbiont-containing tissue for those clams in a sulfide-poor environment, suggesting that the sulfide concentration may be involved in the regulation of these proteins. Gene expression evaluation of the two HbI mRNA variants indicated that the longer variant is expressed at higher levels than the shorter variant in both environments.

  8. Blood pressure reduction due to hemoglobin glycosylation in type 2 diabetic patients

    Directory of Open Access Journals (Sweden)

    Pedro Cabrales

    2008-08-01

    Full Text Available Pedro Cabrales1, Miguel A Salazar Vázquez2,3, Beatriz Y Salazar Vázquez3,4, Martha Rodríguez-Morán5, Marcos Intaglietta4, Fernando Guerrero-Romero51La Jolla Bioengineering Institute, La Jolla, California, USA; 2Hospital Regional No. 1, of the Mexican Social Security Institute, Victoria de Durango, Dgo. Mexico; 3Faculty of Medicine and Dept. of Physical Chemistry, Universidad Juárez del Estado de Durango, Victoria de Durango, Dgo. Mexico; 4Department of Bioengineering, University of California, San Diego, La Jolla, California, USA; 5Biomedical Research Unit, of the Mexican Social Security Institute, Victoria de Durango, Dgo. MexicoObjective: To test the hypothesis that glycosylation of hemoglobin constitutes a risk factor for hypertension.Methods: A total of 129 relative uniform diabetic subjects (86 women and 42 men were enrolled in a cross-sectional study. Exclusion criteria included alcohol consumption, smoking, ischemic heart disease, stroke, neoplasia, renal, hepatic, and chronic inflammatory disease. Systolic and diastolic pressures were recorded in subsequent days and mean arterial blood pressure (MAP was determined. Hemoglobin glycosylation was measured by determining the percentage glycosylated hemoglobin (HbA1c by means of the automated microparticle enzyme immunoassay test.Results: MAP was found to be independent of the concentration of HbA1c; however, correcting MAP for the variability in hematocrit, to evidence the level of vasoconstriction (or vasodilatation showed that MAP is negatively correlated with the concentration of HbA1c (p for trend <0.05, when patients treated for hypertension are excluded from the analysis. Patients treated for hypertension showed the opposite trend with increasing MAP as HbA1c increased (p for the difference in trends <0.05.Conclusions: Glycosylation per se appears to lead to blood pressure reduction in type 2 diabetic patients untreated for hypertension. Treatment for hypertension may be

  9. Education Organization Baseline Control Protection and Trusted Level Security

    OpenAIRE

    Wasim A. Al-Hamdani

    2007-01-01

    Many education organizations have adopted for security the enterprise best practices for implementation on their campuses, while others focus on ISO Standard (or/and) the National Institution of Standards and Technology.All these adoptions are dependent on IT personal and their experiences or knowledge of the standard. On top of this is the size of the education organizations. The larger the population in an education organization, the more the problem of information and security become very ...

  10. 君力达盐酸二甲双胍肠溶胶囊联合阿卡波糖对糖尿病患者空腹及餐后2h血糖、糖化血红蛋白以及甘油三酯水平的影响%Junlida Metformin Hydrochloride Enteric Capsules Connect Acarbose Dia-betes Fasting and Postprandial 2 h Blood Glucose, Glycosylated Hemoglobin and Triglyceride Levels of Influence

    Institute of Scientific and Technical Information of China (English)

    罗云霞; 庞建立

    2016-01-01

    目的:探讨君力达盐酸二甲双胍肠溶胶囊联合阿卡波糖对糖尿病患者空腹及餐后2h血糖、糖化血红蛋白以及甘油三酯水平的影响。方法选取在该科住院治疗的糖尿病患者52例,按照数字随机方式将患者分为对照组和观察组,各组为26例,分别对两组患者进行严格饮食控制及加强锻炼,对照组在此基础上实施君力达盐酸二甲双胍肠溶胶囊予以口服治疗,0.5 g/次,3次/d;观察组患者在上述药物基础上给予联合阿卡波糖50 mg予以治疗,嚼碎吞服,3次/d,持续12周。结果两组患者治疗前,就空腹血糖(FPG)及餐后2 h血糖(2 hPG)、糖化血红蛋白(Hb A1c)以及甘油三酯(TG)水平组间比较无明显差异(P<0.05)。两组患者治疗12周后,其空腹血糖(FPG)及餐后2 h血糖(2 hPG)、糖化血红蛋白(Hb A1c)以及甘油三酯(TG)水平均呈现出明显降低状况,观察组患者治疗后与对照组相比,降低幅度明显高于后者且差异显著(P<0.05);观察组后总有效率(96.15%)与对照组(76.92%)相比,明显高于后者且差异明显(P<0.05);观察组治疗12周后,其血糖控制的疗效与对照组相比,明显高于后者(P<0.05)。结论针对糖尿病患者,在对其进行严格的控制饮食及加强锻炼的基础上,采取君力达盐酸二甲双胍肠溶胶囊联合阿卡波糖进行治疗,其疗效更为确切,能够对患者的血糖水平具有很好的改善效果,有效降低低血糖相应发生率,在临床当中具有很好的应用和推广价值。%Objective To investigate the jun eed metformin hydrochloride enteric capsules joint acarbose fasting and post-prandial 2 h blood glucose in patients with diabetes, the influence of glycosylated hemoglobin and triglyceride levels. Methods Selecting the university hospital treatment of 52 patients with diabetes, according to the number of random, pa-tients can be divided into control group and observation group, each group of 26 cases

  11. Impact of Multi-Micronutrient Fortified Rice on Hemoglobin, Iron and Vitamin A Status of Cambodian Schoolchildren: a Double-Blind Cluster-Randomized Controlled Trial.

    Science.gov (United States)

    Perignon, Marlène; Fiorentino, Marion; Kuong, Khov; Dijkhuizen, Marjoleine A; Burja, Kurt; Parker, Megan; Chamnan, Chhoun; Berger, Jacques; Wieringa, Frank T

    2016-01-07

    In Cambodia, micronutrient deficiencies remain a critical public health problem. Our objective was to evaluate the impact of multi-micronutrient fortified rice (MMFR) formulations, distributed through a World Food Program school-meals program (WFP-SMP), on the hemoglobin concentrations and iron and vitamin A (VA) status of Cambodian schoolchildren. The FORISCA-UltraRice+NutriRice study was a double-blind, cluster-randomized, placebo-controlled trial. Sixteen schools participating in WFP-SMP were randomly assigned to receive extrusion-fortified rice (UltraRice Original, UltraRice New (URN), or NutriRice) or unfortified rice (placebo) six days a week for six months. Four additional schools not participating in WFP-SMP were randomly selected as controls. A total of 2440 schoolchildren (6-16 years old) participated in the biochemical study. Hemoglobin, iron status, estimated using inflammation-adjusted ferritin and transferrin receptors concentrations, and VA status, assessed using inflammation-adjusted retinol-binding protein concentration, were measured at the baseline, as well as at three and six months. Baseline prevalence of anemia, depleted iron stores, tissue iron deficiency, marginal VA status and VA deficiency were 15.6%, 1.4%, 51.0%, 7.9%, and 0.7%, respectively. The strongest risk factors for anemia were hemoglobinopathy, VA deficiency, and depleted iron stores (all p status, respectively, in comparison to the placebo group. Hemoglobin significantly increased (+0.8 g/L) after three months for the URN group in comparison to the placebo group; however, this difference was no longer significant after six months, except for children without inflammation. MMFR containing VA effectively improved the VA status of schoolchildren. The impact on hemoglobin and iron status was limited, partly by sub-clinical inflammation. MMFR combined with non-nutritional approaches addressing anemia and inflammation should be further investigated.

  12. The impact of the detection angle on the quantitative measurement of hemoglobin oxygen saturation in optical-resolution photoacoustic microscopy

    Science.gov (United States)

    Wu, Ning; Li, Changhui

    2016-10-01

    Optical-resolution photoacoustic microscopy (OR-PAM) plays an important role in the quantitative measurement of hemoglobin oxygen saturation (SO2) at a single vessel level. In this study, we reported that the relative angle between light illumination and ultrasonic detection could have a significant impact on the SO2 measurement. Both simulation and phantom studies were provided, and this result will help the system design and result interpretation of the functional OR-PAM.

  13. Biomonitoring of diesel exhaust-exposed workers. DNA and hemoglobin adducts and urinary 1-hydroxypyrene as markers of exposure

    DEFF Research Database (Denmark)

    Nielsen, Per Sabro; Andreassen, Åshild; Farmer, Peter B.;

    1996-01-01

    the 32P-postlabelling method with butanol and P1 enrichment procedures. Hydroxyethylvaline (HOEtVal) adducts in hemoglobin were measured by gas chromatography-mass spectrometry (GC-MS) and 1-hydroxypyrene (HPU) in urine determined using HPLC analysis. The exposed workers had significantly higher levels....... The study indicated that skin absorption of polycyclic aromatic hydrocarbons (PAH) might be an important factor to consider when studying PAH exposure from air pollution sources....

  14. Synthesizing SMOS Zero-Baselines with Aquarius Brightness Temperature Simulator

    Science.gov (United States)

    Colliander, A.; Dinnat, E.; Le Vine, D.; Kainulainen, J.

    2012-01-01

    SMOS [1] and Aquarius [2] are ESA and NASA missions, respectively, to make L-band measurements from the Low Earth Orbit. SMOS makes passive measurements whereas Aquarius measures both passive and active. SMOS was launched in November 2009 and Aquarius in June 2011.The scientific objectives of the missions are overlapping: both missions aim at mapping the global Sea Surface Salinity (SSS). Additionally, SMOS mission produces soil moisture product (however, Aquarius data will eventually be used for retrieving soil moisture too). The consistency of the brightness temperature observations made by the two instruments is essential for long-term studies of SSS and soil moisture. For resolving the consistency, the calibration of the instruments is the key. The basis of the SMOS brightness temperature level is the measurements performed with the so-called zero-baselines [3]; SMOS employs an interferometric measurement technique which forms a brightness temperature image from several baselines constructed by combination of multiple receivers in an array; zero-length baseline defines the overall brightness temperature level. The basis of the Aquarius brightness temperature level is resolved from the brightness temperature simulator combined with ancillary data such as antenna patterns and environmental models [4]. Consistency between the SMOS zero-baseline measurements and the simulator output would provide a robust basis for establishing the overall comparability of the missions.

  15. Concurrent measurement of cellular turbidity and hemoglobin to evaluate the antioxidant activity of plants.

    Science.gov (United States)

    Bellik, Yuva; Iguer-Ouada, Mokrane

    2016-01-01

    In past decades, a multitude of analytical methods for measuring antioxidant activity of plant extracts has been developed. However, when using methods to determine hemoglobin released from human erythrocytes treated with ginger extracts, we found hemoglobin concentrations were significantly higher than in untreated control samples. This suggests in the presence of antioxidants that measuring hemoglobin alone is not sufficient to determine hemolysis. We show concurrent measurement of erythrocyte concentration and hemoglobin is essential in such assays, and describe a new protocol based on simultaneous measurement of cellular turbidity and hemoglobin.

  16. A single hemoglobin gene in Myrica gale retains both symbiotic and non-symbiotic specificity

    DEFF Research Database (Denmark)

    Heckmann, Anne B.; Hebelstrup, Kim Henrik; Larsen, Knud;

    2006-01-01

    Here, a hemoglobin gene from the nitrogen-fixing actinorhizal plant Myrica gale was isolated, cloned and sequenced. The gene (MgHb) was a class I hemoglobin with strong sequence homology to non-symbiotic hemoglobin genes. MgHb is highly expressed in symbiotic root nodules, but transcripts......-symbiotic A. thaliana hemoglobin promoters AHb1 and AHb2. The results points to a symbiotic as well as a non-symbiotic specificity of MgHb similar to a hemoglobin gene identified in Parasponia andersonii, but different from the situation in Casuarina glauca--a close actinorhizal relative of M. gale....

  17. Improvements in or relating to antibodies active against human hemoglobin Asub(1C)

    International Nuclear Information System (INIS)

    A method is described for preparing an antibody against human hemoglobin Asub(1c) which is substantially free of cross-reactivity against the human hemoglobins A0, Asub(1a) and Asub(1b). The antibodies are collected from cats, goats or sheep following injections of purified hemoglobin Asub(1c) antigen since these animals do not naturally produce hemoglobin Asub(1c). A radioimmunoassay method is also described whereby these antibodies are used to determine the quantity of hemoglobin Asub(1c) in blood samples. This is a useful technique in the diagnosis of diabetes mellitus. (U.K.)

  18. Protective Effect of Ascorbic Acid on Molecular Behavior Changes of Hemoglobin Induced by Magnetic Field

    Science.gov (United States)

    Hassan, Nahed S.; Abou Aiad, T. H. M.

    With the use of electricity and industrialization of societies, humans are commonly exposed to static magnetic field induced by electric currents. The putative mechanisms by which Static Magnetic Field (SMF) may affect biological systems is that of increasing free radical life span in organisms. To test this hypothesis, we investigate the effect of ascorbic acid (Vitamin C) treatment on the changes in the molecular behavior of hemoglobin as a result of exposure of the animals to magnetic field in the occupation levels. By measuring the relative permittivity, dielectric loss, relaxation time, conductivity, radius and diffusion coefficient of aqueous solutions of hemoglobin. These measurements were calculated in the frequency range of (100 Hz-100 kHz) to give more information about molecular behavior. Twenty four male albino rats were equally divided into four groups 1, 2, 3 and 4. Animals of group 1, were used as control, animals of group 2, were exposed to (0.2T) magnetic field and that of group 3, 4, were treated with Ascorbic Acid by two doses group 3 (20 mg kg-1 body weight), group 4 (50 mg kg-1 body weight) orally half hour before exposure to magnetic field. The sub chronic exposure expanded (1 h day-1) for 30 consecutive days. The results indicated that exposure of animals to magnetic field resulted in changes in the molecular behavior of hemoglobin molecule while treatment with ascorbic acid afforded comparatively more significant amelioration in these molecular changes, via decreasing the radical pair interaction of magnetic field with biological molecules.

  19. Sub-antimicrobial Doxycycline for Periodontitis Reduces Hemoglobin A1c in Subjects with Type 2 Diabetes: a Pilot Study

    Science.gov (United States)

    Engebretson, Steven P.; Hey-Hadavi, Judith

    2011-01-01

    In vitro and animal studies suggest a possible role for the tetracycline class of drugs in the inhibition of non-enzymatic protein glycation. We conducted a 3-month, randomized placebo-controlled pilot clinical trial of conventional sub-gingival debridement, (periodontal therapy) combined with either a three month regimen of sub-antimicrobial-dose doxycycline (SDD), a two week regimen of antimicrobial-dose doxycycline (ADD), or placebo in 45 patients with long-standing type 2 diabetes (mean duration 9 years) and untreated chronic periodontitis. Subjects were taking stable doses of oral hypoglycemic medications and/or insulin. Treatment response was assessed by measuring hemoglobin A1c (HbA1c),plasma glucose, and clinical periodontal disease measures. At one-month and three-month follow-up, clinical measures of periodontitis were decreased in all groups(data to be presented elsewhere). At three months, mean HbA1c levels in the SDD group were reduced 0.9% unitsfrom 7.2% units ± 2.2(±SD), to 6.3% units ±1.1, which represents a 12.5% improvement. In contrast, there was no significant change in HbA1c in the ADD (7.5%± 2.0 to 7.8%± 2.1) or placebo (8.5%± 2.0 to 8.5%± 2.6) groups. Mean HbA1c change from baseline was significantly greater in the SDD group compared with the ADD group (p=0.04) but not placebo (p=0.22). Moreover, a larger proportion of subjects in the SDD group experienced improvement (p<0.05) compared to the ADD or placebo groups. Mean plasma glucose levels were not significantly different between or within the groups. The results of this pilot study suggest that the treatment of periodontitis with sub-gingival debridement and 3-months of daily sub-antimicrobial-dose doxycycline may decrease HbA1c in patients with type 2 diabetes taking normally prescribed hypoglycemic agents. PMID:21782948

  20. Oxygenation properties of hemoglobin from the turtle Geochelone carbonaria.

    Science.gov (United States)

    Torsoni, M A; Ogo, S H

    1995-01-01

    The oxygen-binding properties of hemoglobin (Hb) from the adult terrestrial turtle Geochelone carbonaria are described. Turtle hemoglobins have a low intrinsic oxygen affinity and a low sensitivity to an endogenous cofactor (ATP) usually present at high concentrations in the reptile erythrocytes. The amplitude of the Bohr effect for O2 binding was virtually the same in the absence and presence of saturating ATP concentrations (delta logP50/delta pH, about -0.60) and increased in the total hemolysate (-0.83). The large Bohr effect found in G. carbonaria Hb may be important for O2 delivery to the tissue. The degree of cooperativity displayed by Hb for O2 binding ranged between 1.5 and 2.0 in stripped solution and total hemolysate. These observations suggest that stability of the low affinity conformation, which needs to be confirmed by additional experiments. PMID:8728839

  1. IsdB-dependent hemoglobin binding is required for acquisition of heme by Staphylococcus aureus.

    Science.gov (United States)

    Pishchany, Gleb; Sheldon, Jessica R; Dickson, Claire F; Alam, Md Tauqeer; Read, Timothy D; Gell, David A; Heinrichs, David E; Skaar, Eric P

    2014-06-01

    Staphylococcus aureus is a Gram-positive pathogen responsible for tremendous morbidity and mortality. As with most bacteria, S. aureus requires iron to cause disease, and it can acquire iron from host hemoglobin. The current model for staphylococcal hemoglobin-iron acquisition proposes that S. aureus binds hemoglobin through the surface-exposed hemoglobin receptor IsdB. IsdB removes heme from bound hemoglobin and transfers this cofactor to other proteins of the Isd system, which import and degrade heme to release iron in the cytoplasm. Here we demonstrate that the individual components of the Isd system are required for growth on low nanomolar concentrations of hemoglobin as a sole source of iron. An in-depth study of hemoglobin binding by IsdB revealed key residues that are required for hemoglobin binding. Further, we show that these residues are necessary for heme extraction from hemoglobin and growth on hemoglobin as a sole iron source. These processes are found to contribute to the pathogenicity of S. aureus in a murine model of infection. Together these results build on the model for Isd-mediated hemoglobin binding and heme-iron acquisition during the pathogenesis of S. aureus infection.

  2. Lower Hemoglobin Concentration Is Associated with Retinal Ischemia and the Severity of Diabetic Retinopathy in Type 2 Diabetes

    Directory of Open Access Journals (Sweden)

    Alicia Traveset

    2016-01-01

    Full Text Available Aims. To assess the association of blood oxygen-transport capacity variables with the prevalence of diabetic retinopathy (DR, retinal ischemia, and macular oedema in patients with type 2 diabetes mellitus (T2DM. Methods. Cross-sectional, case-control study (N=312 with T2DM: 153 individuals with DR and 159 individuals with no DR. Participants were classified according to the severity of DR and the presence of retinal ischemia or macular oedema. Hematological variables were collected by standardized methods. Three logistic models were adjusted to ascertain the association between hematologic variables with the severity of DR and the presence of retinal ischemia or macular oedema. Results. Individuals with severe DR showed significantly lower hemoglobin, hematocrit, and erythrocyte levels compared with those with mild disease and in individuals with retinal ischemia and macular oedema compared with those without these disorders. Hemoglobin was the only factor that showed a significant inverse association with the severity of DR [beta-coefficient = −0.52, P value = 0.003] and retinal ischemia [beta-coefficient = −0.49, P value = 0.001]. Lower erythrocyte level showed a marginally significant association with macular oedema [beta-coefficient = −0.86, P value = 0.055]. Conclusions. In patients with DR, low blood oxygen-transport capacity was associated with more severe DR and the presence of retinal ischemia. Low hemoglobin levels may have a key role in the development and progression of DR.

  3. Lower Hemoglobin Concentration Is Associated with Retinal Ischemia and the Severity of Diabetic Retinopathy in Type 2 Diabetes

    Science.gov (United States)

    Traveset, Alicia; Rubinat, Esther; Ortega, Emilio; Alcubierre, Nuria; Vazquez, Beatriz; Hernández, Marta; Jurjo, Carmen; Espinet, Ramon; Ezpeleta, Juan Antonio; Mauricio, Didac

    2016-01-01

    Aims. To assess the association of blood oxygen-transport capacity variables with the prevalence of diabetic retinopathy (DR), retinal ischemia, and macular oedema in patients with type 2 diabetes mellitus (T2DM). Methods. Cross-sectional, case-control study (N = 312) with T2DM: 153 individuals with DR and 159 individuals with no DR. Participants were classified according to the severity of DR and the presence of retinal ischemia or macular oedema. Hematological variables were collected by standardized methods. Three logistic models were adjusted to ascertain the association between hematologic variables with the severity of DR and the presence of retinal ischemia or macular oedema. Results. Individuals with severe DR showed significantly lower hemoglobin, hematocrit, and erythrocyte levels compared with those with mild disease and in individuals with retinal ischemia and macular oedema compared with those without these disorders. Hemoglobin was the only factor that showed a significant inverse association with the severity of DR [beta-coefficient = −0.52, P value = 0.003] and retinal ischemia [beta-coefficient = −0.49, P value = 0.001]. Lower erythrocyte level showed a marginally significant association with macular oedema [beta-coefficient = −0.86, P value = 0.055]. Conclusions. In patients with DR, low blood oxygen-transport capacity was associated with more severe DR and the presence of retinal ischemia. Low hemoglobin levels may have a key role in the development and progression of DR. PMID:27200379

  4. Liposomes surface conjugated with human hemoglobin target delivery to macrophages

    OpenAIRE

    Zhang, Ning; Palmer, Andre F.

    2011-01-01

    Current strategies to deliver therapeutic molecules to specific cell and tissue types rely on conjugation of antibodies and other targeting ligands directly to the therapeutic molecule itself or its carrier. This work describes a novel strategy to deliver therapeutic molecules into macrophages that takes advantage of the native hemoglobin (Hb) scavenging activity of plasma haptoglobin (Hp) and the subsequent uptake of the Hb-Hp complex into macrophages via CD163 receptor mediated endocytosis....

  5. Allylation of Intraerythrocytic Hemoglobin by Raw Garlic Extracts

    OpenAIRE

    Bonaventura, Joseph; Rodriguez, Eva N.; Beyley, Veronica; Vega, Irving E.

    2010-01-01

    Recent studies have shown that deoxygenated human red blood cells (RBCs) converted garlic-derived polysulfides into hydrogen sulfide, which in turn produced vasorelaxation in aortic ring preparations. The vasoactivity was proposed to occur via glucose- and thiol-dependent acellular reactions. In the present study, we investigated the interaction of garlic extracts with human deoxygenated RBCs and its effect on intracellular hemoglobin molecules. The results showed that garlic extract covalent...

  6. Optical Spectra of Hemoglobin Taken from Alcohol Dependent Humans

    OpenAIRE

    Dudok K.; Dudok T.; Vlokh I.; Vlokh R.

    2005-01-01

    Optical spectra of CNMetHb and CNMetHb-Coomassi G-250, taken from the blood of humans with alcohol dependence, are studied in the spectral range of 450–750nm. The shifts in the spectral absorption maxima of CNMetHb-Coomassi G-250 complexes are observed for the diseased persons with alcohol dependence. The obtained results show that the hemoglobin structure of alcohol dependent humans is changed.

  7. Reticulocyte hemoglobin content as a predictor of iron deficiency anemia

    Directory of Open Access Journals (Sweden)

    Ni Made Rini Suari

    2015-05-01

    Full Text Available Background Iron deficiency anemia (IDA is the most common form of anemia in developing countries, such as Indonesia. Iron deficiency anemia in children is a serious problem because it affects their growth and development. Early detection of IDA and subsequent treatment in childhood may prevent future health problems.Objective To assess the use of reticulocyte hemoglobin content (CHr to detect IDA in children aged 6-60 months.Methods We performed a cross-sectional study to measure the sensitivity and specificity of CHr compared to serum ferritin which is considered to be the gold standard for IDA diagnosis. The study was conducted from September 2011 to March 2013 in children aged 6-60 months who visited the Pediatric Outpatient Clinic, Sanglah Hospital, and Puskesmas II in West Denpasar. Data analysis was performed by 2x2 table. The results were assessed by area under the curve (AUC and receiver operating characteristic (ROC.Results Of 121 children underwent blood testing during the study period, 69 children were excluded because they did not have hypochromic microcytic anemia, leaving 52 subjects eligible for the study. The prevalence of IDA in this study was 31%. Reticulocyte hemoglobin content (CHr ≤ 23.1 pg had 88% (95%CI 71 to 100% sensitivity and 25% (95%CI 11 to 39% specificity.Conclusion Reticulocyte hemoglobin content < 23.1 pg may be a good predictor of IDA.

  8. Reference intervals for acetylated fetal hemoglobin in healthy newborns

    Directory of Open Access Journals (Sweden)

    Renata Paleari

    2014-09-01

    Full Text Available The acetylated fetal hemoglobin (AcHbF derives from an enzyme-mediated post-translational modification occurring on the N-terminal glycine residues of γ-chains. At present, no established data are available on reference intervals for AcHbF in newborns. A total of 92 healthy infants, with gestational age between 37 and 41 weeks were selected for the establishment of AcHbF reference intervals. Blood samples were collected by heel pricking, when collecting routine neonatal screening, and the hemoglobin pattern was analyzed by high-performance liquid chromatography. AcHbF results were then normalized for HbF content in order to account for differences in hemoglobin switch. No difference was found in AcHbF values between genders (P=0.858. AcHbF results were as follow: 12.8±0.8% (mean±standard deviation, reference interval: 11.3-14.3%. This finding could facilitate further studies aimed to assess the possible use of AcHbF, for instance as a possible fetal metabolic biomarker during pregnancy.

  9. Switching patients with non-dialysis chronic kidney disease from oral iron to intravenous ferric carboxymaltose: effects on erythropoiesis-stimulating agent requirements, costs, hemoglobin and iron status.

    Directory of Open Access Journals (Sweden)

    Jorge Eduardo Toblli

    Full Text Available Patients with non-dialysis-dependent chronic kidney disease (ND-CKD often receive an erythropoiesis-stimulating agent (ESA and oral iron treatment. This study evaluated whether a switch from oral iron to intravenous ferric carboxymaltose can reduce ESA requirements and improve iron status and hemoglobin in patients with ND-CKD.This prospective, single arm and single-center study included adult patients with ND-CKD (creatinine clearance ≤40 mL/min, hemoglobin 11-12 g/dL and iron deficiency (ferritin 20%. Outcome measures were ESA dose requirements during the observation period after initial ferric carboxymaltose treatment (primary endpoint; number of hospitalizations and transfusions, renal function before and after ferric carboxymaltose administration, number of adverse reactions (secondary endpoints. Hemoglobin, mean corpuscular volume, ferritin and transferrin saturation were measured monthly from baseline until end of study. Creatinine clearance, proteinuria, C-reactive protein, aspartate aminotransferase, alanine aminotransferase and alkaline phosphatase bimonthly from baseline until end of study.Thirty patients were enrolled (age 70.1±11.4 years; mean±SD. Mean ESA consumption was significantly reduced by 83.2±10.9% (from 41,839±3,668 IU/patient to 6,879±4,271 IU/patient; p<0.01. Hemoglobin increased by 0.7±0.3 g/dL, ferritin by 196.0±38.7 μg/L and transferrin saturation by 5.3±2.9% (month 6 vs. baseline; all p<0.01. No ferric carboxymaltose-related adverse events were reported and no patient withdrew or required transfusions during the study.Among patients with ND-CKD and stable normal or borderline hemoglobin, switching from oral iron to intravenous ferric carboxymaltose was associated with significant improvements in hematological and iron parameters and a significant reduction in ESA dose requirements in this single-center pilot study.ClinicalTrials.gov NCT02232906.

  10. Switching Patients with Non-Dialysis Chronic Kidney Disease from Oral Iron to Intravenous Ferric Carboxymaltose: Effects on Erythropoiesis-Stimulating Agent Requirements, Costs, Hemoglobin and Iron Status

    Science.gov (United States)

    Toblli, Jorge Eduardo; Di Gennaro, Federico

    2015-01-01

    Background Patients with non-dialysis-dependent chronic kidney disease (ND-CKD) often receive an erythropoiesis-stimulating agent (ESA) and oral iron treatment. This study evaluated whether a switch from oral iron to intravenous ferric carboxymaltose can reduce ESA requirements and improve iron status and hemoglobin in patients with ND-CKD. Methods This prospective, single arm and single-center study included adult patients with ND-CKD (creatinine clearance ≤40 mL/min), hemoglobin 11–12 g/dL and iron deficiency (ferritin 20%). Outcome measures were ESA dose requirements during the observation period after initial ferric carboxymaltose treatment (primary endpoint); number of hospitalizations and transfusions, renal function before and after ferric carboxymaltose administration, number of adverse reactions (secondary endpoints). Hemoglobin, mean corpuscular volume, ferritin and transferrin saturation were measured monthly from baseline until end of study. Creatinine clearance, proteinuria, C-reactive protein, aspartate aminotransferase, alanine aminotransferase and alkaline phosphatase bimonthly from baseline until end of study. Results Thirty patients were enrolled (age 70.1±11.4 years; mean±SD). Mean ESA consumption was significantly reduced by 83.2±10.9% (from 41,839±3,668 IU/patient to 6,879±4,271 IU/patient; p<0.01). Hemoglobin increased by 0.7±0.3 g/dL, ferritin by 196.0±38.7 μg/L and transferrin saturation by 5.3±2.9% (month 6 vs. baseline; all p<0.01). No ferric carboxymaltose-related adverse events were reported and no patient withdrew or required transfusions during the study. Conclusion Among patients with ND-CKD and stable normal or borderline hemoglobin, switching from oral iron to intravenous ferric carboxymaltose was associated with significant improvements in hematological and iron parameters and a significant reduction in ESA dose requirements in this single-center pilot study. Trial Registration ClinicalTrials.gov NCT02232906 PMID

  11. SRS baseline hydrogeologic investigation: Summary report

    Energy Technology Data Exchange (ETDEWEB)

    Bledsoe, H.W.; Aadland, R.K. [Westinghouse Savannah River Co., Aiken, SC (United States); Sargent, K.A. [Furman Univ., Greenville, SC (United States). Dept. of Geology

    1990-11-01

    Work on the Savannah River Site (SRS) Baseline Hydrogeologic Investigation began in 1983 when it was determined that the knowledge of the plant hydrogeologic systems needed to be expanded and improved in response to changing stratigraphic and hydrostratigraphic terminology and increased involvement by regulatory agencies (Bledsoe, 1984). Additionally, site-wide data were needed to determine flow paths, gradients, and velocities associated with the different aquifers underlying the plant site. The program was divided into three phases in order to allow the results of one phase to be evaluated and necessary changes and improvements incorporated into the following phases. This report summarizes the results of all three phases and includes modified graphic logs, lithologic descriptions of the different geologic formations, profiles of each cluster site, hydrostratigraphic cross sections, hydrographs of selected wells within each cluster for the first full year of uninterrupted water level measurements, potentiometric maps developed from data collected from all clusters, completion diagrams for each well, and a summary of laboratory tests. Additionally, the proposed new classification of hydrostratigraphic units at SRS (Aadland and Bledsoe, 1990) has been incorporated.

  12. Lorentz symmetry and Very Long Baseline Interferometry

    CERN Document Server

    Poncin-Lafitte, C Le; lambert, S

    2016-01-01

    Lorentz symmetry violations can be described by an effective field theory framework that contains both General Relativity and the Standard Model of particle physics called the Standard-Model extension (SME). Recently, post-fit analysis of Gravity Probe B and binary pulsars lead to an upper limit at the $10^{-4}$ level on the time-time coefficient $\\bar s^{TT}$ of the pure-gravity sector of the minimal SME. In this work, we derive the observable of Very Long Baseline Interferometry (VLBI) in SME and then we implement it into a real data analysis code of geodetic VLBI observations. Analyzing all available observations recorded since 1979, we compare estimates of $\\bar s^{TT}$ and errors obtained with various analysis schemes, including global estimations over several time spans and with various Sun elongation cut-off angles, and with analysis of radio source coordinate time series. We obtain a constraint on $\\bar s^{TT}=(-5\\pm 8)\\times 10^{-5}$, directly fitted to the observations and improving by a factor 5 pr...

  13. Non-invasive measurement of hemoglobin: assessment of two different point-of-care technologies.

    Directory of Open Access Journals (Sweden)

    Etienne Gayat

    Full Text Available BACKGROUND: Measurement of blood hemoglobin (Hb concentration is a routine procedure. Using a non-invasive point-of-care device reduces pain and discomfort for the patient and allows time saving in patient care. The aims of the present study were to assess the concordance of Hb levels obtained non-invasively with the Pronto-7 monitor (version 2.1.9, Masimo Corporation, Irvine, USA or with the NBM-200MP monitor (Orsense, Nes Ziona, Israel and the values obtained from the usual colorimetric method using blood samples and to determine the source of discordance. METHODS AND FINDINGS: We conducted two consecutive prospective open trials enrolling patients presenting in the emergency department of a university hospital. The first was designed to assess Pronto-7™ and the second NBM-200MP™. In each study, the main outcome measure was the agreement between both methods. Independent factors associated with the bias were determined using multiple linear regression. Three hundred patients were prospectively enrolled in each study. For Pronto-7™, the absolute mean difference was 0.56 g.L(-1 (95% confidence interval [CI] 0.41 to 0.69 with an upper agreement limit at 2.94 g.L(-1 (95% CI [2.70;3.19], a lower agreement limit at -1.84 g.L(-1 (95% CI [-2.08;-1.58] and an intra-class correlation coefficient at 0.80 (95% CI [0.74;0.84]. The corresponding values for the NBM-200MP™ were 0.21 [0.02;0.39], 3.42 [3.10;3.74], -3.01 [-3.32;-2.69] and 0.69 [0.62;0.75]. Multivariate analysis showed that age and laboratory values of hemoglobin were independently associated with the bias when using Pronto-7™, while perfusion index and laboratory value of hemoglobin were independently associated with the bias when using NBM-200MP™. CONCLUSION: Despite a relatively limited bias in both cases, the large limits of agreement found in both cases render the clinical usefulness of such devices debatable. For both devices, the bias is independently and inversely associated

  14. SENP1, but not fetal hemoglobin, differentiates Andean highlanders with chronic mountain sickness from healthy individuals among Andean highlanders.

    Science.gov (United States)

    Hsieh, Matthew M; Callacondo, David; Rojas-Camayo, Jose; Quesada-Olarte, Jose; Wang, Xunde; Uchida, Naoya; Maric, Irina; Remaley, Alan T; Leon-Velarde, Fabiola; Villafuerte, Francisco C; Tisdale, John F

    2016-06-01

    Chronic mountain sickness (CMS) results from chronic hypoxia. It is unclear why certain highlanders develop CMS. We hypothesized that modest increases in fetal hemoglobin (HbF) are associated with lower CMS severity. In this cross-sectional study, we found that HbF levels were normal (median = 0.4%) in all 153 adult Andean natives in Cerro de Pasco, Peru. Compared with healthy adults, the borderline elevated hemoglobin group frequently had symptoms (headaches, tinnitus, cyanosis, dilatation of veins) of CMS. Although the mean hemoglobin level differed between the healthy (17.1 g/dL) and CMS (22.3 g/dL) groups, mean plasma erythropoietin (EPO) levels were similar (healthy, 17.7 mIU/mL; CMS, 12.02 mIU/mL). Sanger sequencing determined that single-nucleotide polymorphisms in endothelial PAS domain 1 (EPAS1) and egl nine homolog 1 (EGLN1), associated with lower hemoglobin in Tibetans, were not identified in Andeans. Sanger sequencing of sentrin-specific protease 1 (SENP1) and acidic nuclear phosphoprotein 32 family, member D (ANP32D), in healthy and CMS individuals revealed that non-G/G genotypes were associated with higher CMS scores. No JAK2 V617F mutation was detected in CMS individuals. Thus, HbF and other classic erythropoietic parameters did not differ between healthy and CMS individuals. However, the non-G/G genotypes of SENP1 appeared to differentiate individuals with CMS from healthy Andean highlanders.

  15. Life Support Baseline Values and Assumptions Document

    Science.gov (United States)

    Anderson, Molly S.; Ewert, Michael K.; Keener, John F.; Wagner, Sandra A.

    2015-01-01

    The Baseline Values and Assumptions Document (BVAD) provides analysts, modelers, and other life support researchers with a common set of values and assumptions which can be used as a baseline in their studies. This baseline, in turn, provides a common point of origin from which many studies in the community may depart, making research results easier to compare and providing researchers with reasonable values to assume for areas outside their experience. With the ability to accurately compare different technologies' performance for the same function, managers will be able to make better decisions regarding technology development.

  16. Mixed waste focus area technical baseline report. Volume 1

    International Nuclear Information System (INIS)

    The Department of Energy (DOE) established the Mixed Waste Characterization, Treatment, and Disposal Focus Area (MWFA) to develop and facilitate implementation of technologies required to meet the Department's commitments for treatment of mixed low-level and transuranic wastes. The mission of the MWFA is to provide acceptable technologies, developed in partnership with end-users, stakeholders, tribal governments, and regulators, that enable implementation of mixed waste treatment systems. To accomplish this mission, a technical baseline was established in 1996 that forms the basis for determining which technology development activities will be supported by the MWFA. This technical baseline is revised on an annual basis to reflect changes in the DOE Mixed Waste Management strategies, changes in the MWFA technical baseline development process, and MWFA accomplishments. This report presents the first revision to the technical baseline and the resulting prioritized list of deficiencies that the MWFA will address. This report also reflects a higher level of stakeholder involvement in the prioritization of the deficiencies. This document summarizes the data and the assumptions upon which this work was based, as well as information concerning the DOE Office of Environmental Management (EM) mixed waste technology development needs

  17. Impacto da farinha de mandioca fortificada com ferro aminoácido quelato no nível de hemoglobina de pré-escolares Impact of cassava flour fortified with iron amino acid chelate on the hemoglobin level in pre-schools

    Directory of Open Access Journals (Sweden)

    Rahilda Brito Tuma

    2003-01-01

    Full Text Available OBJETIVO: Avaliou-se o impacto da farinha de mandioca fortificada com ferro aminoácido quelato em 80 pré-escolares de uma Unidade Filantrópica de Manaus, AM, distribuídos aleatoriamente em quatro grupos de 20 crianças cada, por um período de 120 dias. MÉTODOS: Foram utilizadas farinha de mandioca sem fortificação (Grupo zero e fortificada com 1, 2 e 3mg de Fe/dia, correspondendo a quantias diárias de 5, 10 e 15g de farinha, respectivamente, as quais foram distribuídas no horário do almoço, sendo ainda entregue às famílias a quantidade destinada ao consumo do final de semana. O estado nutricional das crianças foi avaliado no início e ao final do experimento, adotando-se como limite discriminatório entre eutrofia/desnutrição o ponto de corte OBJECTIVE: The impact of the cassava flour fortified with iron amino acid chelate was evaluated in 80 pre-scholars of a Philanthropic Unit of Manaus, state of Amazonas, randomly distributed in four groups of 20 children each, for a period of 120 days. METHODS: Cassava flour was used without fortification (group zero or fortified with 1, 2 and 3mg of Fe/day, corresponding respectively to 5, 10 and 15g of flour/day, which were given to the children at lunch time on weekdays. The equivalent amount was previously distributed to their families for flour intake also during the weekends. In the beginning and at the end of the experiment the children's nutritional status was evaluated, being adopted the cutoff point <-2 Z-scores as a discriminating limit between eutrophy/malnutrition, in agreement with the World Health Organization criteria, as well as being established as a cutoff point for the occurrence of iron deficiency anemia a hemoglobin rate of less than 11g/dL. RESULTS: At the end of this study, children recovered from chronic malnutrition, and a significant increase (p <5% of the hemoglobin rates, independently of iron concentration, from 11.4±0.9g/dL to 12.2±0.8g/dL, was observed in

  18. Hemoglobin redux: combining neutron and X-ray diffraction with mass spectrometry to analyse the quaternary state of oxidized hemoglobins

    Energy Technology Data Exchange (ETDEWEB)

    Mueser, Timothy C., E-mail: timothy.mueser@utoledo.edu; Griffith, Wendell P. [Department of Chemistry, University of Toledo, Toledo, OH 43606 (United States); Kovalevsky, Andrey Y. [Bioscience Division, MS M888, Los Alamos National Laboratory, Los Alamos, NM 87545 (United States); Guo, Jingshu; Seaver, Sean [Department of Chemistry, University of Toledo, Toledo, OH 43606 (United States); Langan, Paul [Department of Chemistry, University of Toledo, Toledo, OH 43606 (United States); Bioscience Division, MS M888, Los Alamos National Laboratory, Los Alamos, NM 87545 (United States); Hanson, B. Leif [Department of Chemistry, University of Toledo, Toledo, OH 43606 (United States)

    2010-11-01

    X-ray and neutron diffraction studies of cyanomethemoglobin are being used to evaluate the structural waters within the dimer–dimer interface involved in quaternary-state transitions. Improvements in neutron diffraction instrumentation are affording the opportunity to re-examine the structures of vertebrate hemoglobins and to interrogate proton and solvent position changes between the different quaternary states of the protein. For hemoglobins of unknown primary sequence, structural studies of cyanomethemoglobin (CNmetHb) are being used to help to resolve sequence ambiguity in the mass spectra. These studies have also provided additional structural evidence for the involvement of oxidized hemoglobin in the process of erythrocyte senescence. X-ray crystal studies of Tibetan snow leopard CNmetHb have shown that this protein crystallizes in the B state, a structure with a more open dyad, which possibly has relevance to RBC band 3 protein binding and erythrocyte senescence. R-state equine CNmetHb crystal studies elaborate the solvent differences in the switch and hinge region compared with a human deoxyhemoglobin T-state neutron structure. Lastly, comparison of histidine protonation between the T and R state should enumerate the Bohr-effect protons.

  19. Enfermedad por Hemoglobina H: primer caso de la variante de hemoglobina H tipo (-α3.7/ --SEA en Costa Rica. Hemoglobin H Disease: First Case of Hemoglobin H Variant Type (-α<3.7/ --SEA in Costa Rica

    Directory of Open Access Journals (Sweden)

    Walter Cartín-Sánchez

    2010-07-01

    hemoglobin levels around 9 to 10 g/dL however, during a hemolytic crisis, which frequently occur in or after acute infections causing high fever, the hemoglobin may drop significantly and the patients can develop shock or renal shutdown. Even though splenectomy leads to significant elevation of hemoglobin levels, it is not recommended because the majority of patients do well with said steady-state hemoglobin levels. We present here the first case of hemoglobin H (-α3.7/ --SEA southeast Asia variant described in Costa Rica.¹

  20. SSA FITARA Common Baseline Implementation Plan

    Data.gov (United States)

    Social Security Administration — This document describes the agency's plan to implement the Federal Information Technology Acquisition Reform Act (FITARA) Common Baseline per OMB memorandum M-15-14.

  1. Breton Island, Louisiana Baseline (Geographic, NAD83)

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — Breton Island, Louisiana Baseline (Geographic, NAD83) consists of vector line data that were input into the Digital Shoreline Analysis System (DSAS) version 4.0,...

  2. Monoenergetic neutrino beam for long baseline experiments

    International Nuclear Information System (INIS)

    In an electron capture process by a nucleus, emitted neutrinos are monoenergetic. We study a long baseline experiment with such a completely monoenergetic neutrino beam. This talk is based on Refs. 1 and 2. (author)

  3. Changes in the Conformational State of Hemoglobin in Hemodialysed Patients with Chronic Renal Failure

    Directory of Open Access Journals (Sweden)

    Anna Pieniazek

    2015-01-01

    Full Text Available The aim of this study was to evaluate the properties of internal components of erythrocytes in chronic renal failure (CRF patients undergoing hemodialysis (HD in comparison to control subjects. For investigation of conformational state of hemoglobin and nonheme proteins (NHP the maleimide spin label (MSL in electron paramagnetic resonance (EPR was applied. The studies were performed using MSL in whole cells and hemolysate as well as proteins separated by ion exchange chromatography and checked by electrophoresis. Additionally the level of –SH groups in hemolysate and isolated internal proteins of CRF erythrocytes was determined using 4,4′-dithiodipyridine. All measurements were performed before and after hemodialysis. Oxidative stress accompanying CRF/hemodialysed patients caused a significant decrease in the mobility of internal components inside erythrocytes indicated by MSL (P < 0.02. The significant decrease in mobility of spin labeled HbA1c and HbA both before and after HD (P < 0.0002 as well as in nonheme proteins before hemodialysis (P < 0.05 versus control was indicated. Decrease in mobility of internal components of erythrocytes was accompanied by loss of thiols before and after hemodialysis versus control in NHP (P < 0.05, HbA1c (P < 0.0002, and HbA (P < 0.0005. These findings showed oxidative influence of hemodialysis on hemoglobins and internal nonheme proteins in erythrocytes of CRF patients.

  4. Blood leptin levels and erythropoietin requirement in Iranian hemodialysis patients

    Directory of Open Access Journals (Sweden)

    Rahimi A

    2008-12-01

    Full Text Available "nBackground: Anemia is a common complication accompanied by high morbidity and mortality in hemodialysis patients. Considering the fact that the reduction of erythropoietin (EPO synthesis is the main cause of uremic anemia, receiving recombinant human erythropoietin (rHuEPO can improve the condition in these patients. Some of these hemodialysis patients, however, have acceptable hemoglobin levels without any need to EPO. Higher BMI, higher albumin and leptin plasma levels and longer durations of hemodialysis are possible factors contributing to the reduced need for rHuEPO in these patients. The present study is designed to asses the relationship between the plasma levels of leptin and the reduced EPO need. "nMethods: Fifty eligible hemodialysis patients with hemoglobin levels higher than 11 mg/dl were enrolled in the cross-sectional study. The information on age, sex, hemodialysis duration and the cause of renal dysfunction were extracted from the files. The baseline plasma levels of Leptin and albumin were measured. The patients BMI and the weekly need for rHuEPO were also calculated. "nResults: There was no correlation between the weekly need for rHuEPO and sex, BMI, the cause of renal dysfunction and the plasma levels of albumin and leptin; it, however, was related with age and the duration of dialysis. While age negatively influences the weekly need, the duration of dialysis has a positive effect on the need. "nConclusion: The plasma levels of leptin are not directly correlated with the required amounts of rHuEPO, indicating that leptin is not an effective factor in erythropoiesis. Conversely, older age and shorter hemodialysis durations are accompanied by reduced need for rHuEPO.

  5. Nonintrusive methodology for wellness baseline profiling

    Science.gov (United States)

    Chung, Danny Wen-Yaw; Tsai, Yuh-Show; Miaou, Shaou-Gang; Chang, Walter H.; Chang, Yaw-Jen; Chen, Shia-Chung; Hong, Y. Y.; Chyang, C. S.; Chang, Quan-Shong; Hsu, Hon-Yen; Hsu, James; Yao, Wei-Cheng; Hsu, Ming-Sin; Chen, Ming-Chung; Lee, Shi-Chen; Hsu, Charles; Miao, Lidan; Byrd, Kenny; Chouikha, Mohamed F.; Gu, Xin-Bin; Wang, Paul C.; Szu, Harold

    2007-04-01

    We develop an accumulatively effective and affordable set of smart pair devices to save the exuberant expenditure for the healthcare of aging population, which will not be sustainable when all the post-war baby boomers retire (78 millions will cost 1/5~1/4 GDP in US alone). To design an accessible test-bed for distributed points of homecare, we choose two exemplars of the set to demonstrate the possibility of translation of modern military and clinical know-how, because two exemplars share identically the noninvasive algorithm adapted to the Smart Sensor-pairs for the real world persistent surveillance. Currently, the standard diagnoses for malignant tumors and diabetes disorders are blood serum tests, X-ray CAT scan, and biopsy used sometime in the physical checkup by physicians as cohort-average wellness baselines. The loss of the quality of life in making second careers productive may be caused by the missing of timeliness for correct diagnoses and easier treatments, which contributes to the one quarter of human errors generating the lawsuits against physicians and hospitals, which further escalates the insurance cost and wasteful healthcare expenditure. Such a vicious cycle should be entirely eliminated by building an "individual diagnostic aids (IDA)," similar to the trend of personalized drug, developed from daily noninvasive intelligent databases of the "wellness baseline profiling (WBP)". Since our physiology state undulates diurnally, the Nyquist anti-aliasing theory dictates a minimum twice-a-day sampling of the WBP for the IDA, which must be made affordable by means of noninvasive, unsupervised and unbiased methodology at the convenience of homes. Thus, a pair of military infrared (IR) spectral cameras has been demonstrated for the noninvasive spectrogram ratio test of the spontaneously emitted thermal radiation from a normal human body at 37°C temperature. This invisible self-emission spreads from 3 microns to 12 microns of the radiation wavelengths

  6. Confirmation of Structural Variants of Hemoglobin Using Acid Gel in Hydrasis Technology

    Directory of Open Access Journals (Sweden)

    Jacqueline Pérez Rodríguez

    2013-07-01

    Full Text Available Background: The National Medical Genetics Center has conducted a research on the major hemoglobin abnormalities of clinical interest (HbS, HbC present in pregnant women in the province of Artemisa, using Hydrasis technology with alkaline agarose gels. The use of these gels does not allow distinguishing hemoglobin S from hemoglobin D or hemoglobin C from hemoglobin E, as these hemoglobins migrate in the same position. Objective: to assess the use of acid gel to differentiate haemoglobin D from haemoglobin E by their mobility in hemoglobin electrophoresis. Methods: a descriptive study was conducted using hemoglobin electrophoresis with Hydrasis technology to analyze 200 biological samples of whole blood from pregnant women and individuals with sicklemia or carrying the disease. The acceptance limits included a correlation of electrophoresis run in acid gel in relation to alkaline gel, b correct migration of the hemoglobin bands and c good interpretation of the results. Results: 98 % of the samples analyzed with acid gel showed correspondence with those obtained with alkaline gel. It was not possible to determine and confirm the hemoglobin variant in three samples. Conclusions: the use of acid gels in electrophoresis run with the Hydrasis technology provided results which confirm the diagnosis of hemoglobinopathies.

  7. Specific induction of fibronectin binding activity by hemoglobin in Candida albicans grown in defined media.

    Science.gov (United States)

    Yan, S; Nègre, E; Cashel, J A; Guo, N; Lyman, C A; Walsh, T J; Roberts, D D

    1996-08-01

    Fibronectin (FN) is a major component of host extracellular matrix that may play an important role in the initiation and dissemination of Candida albicans infections. Expression of FN binding requires growth of C albicans blastoconidia in complex medium, and the regulation of FN receptor expression is poorly understood. We now demonstrate that hemoglobin is a potent and specific inducer of FN receptor expression and describe a defined medium supplemented with hemoglobin that greatly and stably enhances the binding activity of C. albicans for soluble FN. Enhancement of FN binding by hemoglobin in strain 44807 was concentration dependent and was maximal at 0.1% hemoglobin with 20- to 80-fold enhancement. The hemoglobin-induced FN binding to C. albicans was saturable, with a Kd of 2.7 X 10(-8) M. Enhancement required growth of C. albicans in hemoglobin-containing medium, since simply exposing blastoconidia to hemoglobin in a nongrowing status did not enhance binding. Induction was reversible following removal of hemoglobin from the growth medium and not associated with germination. Inorganic or protein-bound iron was not sufficient for the induction, since other iron-containing proteins or inorganic iron salts were inactive. Growth in the simple medium yeast nitrogen base supplemented with hemoglobin increased cell adhesion to immobilized FN and to cultured monolayers of bovine corneal endothelial cells. These data suggest that hemoglobin may be an important regulator of FN binding activity in C. albicans and thus may play a role in its pathogenesis. PMID:8757815

  8. Genetic differences in hemoglobin function between highland and lowland deer mice

    DEFF Research Database (Denmark)

    Storz, Jay F.; Runck, Amy M.; Moriyama, Hideaki;

    2010-01-01

    In high-altitude vertebrates, adaptive changes in blood–O2 affinity may be mediated by modifications of hemoglobin (Hb) structure that affect intrinsic O2 affinity and/or responsiveness to allosteric effectors that modulate Hb–O2 affinity. This mode of genotypic specialization is considered typical...... of mammalian species that are high-altitude natives. Here we investigated genetically based differences in Hb–O2 affinity between highland and lowland populations of the deer mouse (Peromyscus maniculatus), a generalist species that has the broadest altitudinal distribution of any North American mammal....... The results of a combined genetic and proteomic analysis revealed that deer mice harbor a high level of Hb isoform diversity that is attributable to allelic polymorphism at two tandemly duplicated -globin genes and two tandemly duplicated β-globin genes. This high level of isoHb diversity translates...

  9. Atlantic cod (Gadus morhua hemoglobin genes: multiplicity and polymorphism

    Directory of Open Access Journals (Sweden)

    Gamperl A Kurt

    2009-09-01

    Full Text Available Abstract Background Hemoglobin (Hb polymorphism, assessed by protein gel electrophoresis, has been used almost exclusively to characterize the genetic structure of Atlantic cod (Gadus morhua populations and to establish correlations with phenotypic traits such as Hb oxygen binding capacity, temperature tolerance and growth characteristics. The genetic system used to explain the results of gel electrophoresis entails the presence of one polymorphic locus with two major alleles (HbI-1; HbI-2. However, vertebrates have more than one gene encoding Hbs and recent studies have reported that more than one Hb gene is present in Atlantic cod. These observations prompted us to re-evaluate the number of Hb genes expressed in Atlantic cod, and to perform an in depth search for polymorphisms that might produce relevant phenotypes for breeding programs. Results Analysis of Expressed Sequence Tags (ESTs led to the identification of nine distinct Hb transcripts; four corresponding to the α Hb gene family and five to the β Hb gene family. To gain insights about the Hb genes encoding these transcripts, genomic sequence data was generated from heterozygous (HbI-1/2 parents and fifteen progeny; five of each HbI type, i.e., HbI-1/1, HbI-1/2 and HbI-2/2. β Hb genes displayed more polymorphism than α Hb genes. Two major allele types (β1A and β1B that differ by two linked non-synonymous substitutions (Met55Val and Lys62Ala were found in the β1 Hb gene, and the distribution of these β1A and β1B alleles among individuals was congruent with that of the HbI-1 and HbI-2 alleles determined by protein gel electrophoresis. RT-PCR and Q-PCR analysis of the nine Hb genes indicates that all genes are expressed in adult fish, but their level of expression varies greatly; higher expression of almost all Hb genes was found in individuals displaying the HbI-2/2 electrophoretic type. Conclusion This study indicates that more Hb genes are present and expressed in adult

  10. 新诊糖尿病合并肺部感染与血糖及糖化血红蛋白水平切点的探讨%Exploration of new diagnosis of diabetes complicated with pulmonary infection and level point of blood glucose and glycated hemoglobin

    Institute of Scientific and Technical Information of China (English)

    刘长江; 徐美华; 饶小胖

    2016-01-01

    目的:探讨新诊糖尿病合并肺部感染与血糖及糖化血红蛋白( HbAlc)的相关性及切点。方法回顾性分析2012年至2015年入住我院内分泌科340例糖尿病合并肺部感染患者为的临床资料,所有研究对象均记录年龄、性别,测量身高、体质量,腰围(WC)、收缩压(SBP)和舒张压(DBP),同时测定各项生化指标,采用SPSS17.0统计软件进行统计学分析,通过绘制受试者工作( ROC)曲线,确定空腹血糖(FBG)、HbAlc的最佳切点。结果①感染组的HbAlc(8.1±0.7)、FBG(9.7±1.6)均明显高于非感染组的HbAlc(6.7±0.4)、FBG(7.3±1.8),P<0.001,感染组的年龄(52.8±7.9)、BMI(28.2±3.6)均高于非感染组的年龄(46.9±7.2)、BMI(22.8±3.6),P<0.05;②以糖尿病对肺部感染患病影响明显的为HbAlc(相关性数B=6.623,OR=9.230,95%CI 4.438~25.62,P<0.0001),其次为FBG(相关性数B=0.542,OR=1.15,95%CI 1.052~1.224,P=0.013),再者为BMI(相关性数B=0.203,OR=1.850,95%CI 1.039~2.542, P=0.024)及年龄(相关性数B=0.042,OR=1.041,95%CI 1.020~1.072,P=0.028);③受试者工作( ROC)曲线分析HbAlc 、FBG及BMI等指标的预测肺部感染增加的曲线下面积,分别为0.717、0.663及0.569, HbAlc、FBG及BMI等指标的预测肺部感染增加的最佳切割点,分别为8.16%、9.82及27.8。结论新诊糖尿病患者的肺部感染与空腹血糖、糖化血红蛋白及年龄和体质量指数有关;新诊DM肺部感染的空腹血糖切点为9.82 mmol/L,糖化血红蛋白切点为8.16%。%Objective To explore a new diagnosis of diabetes mellitus complicated with pulmonary infection and the cutoff point of blood glucose and glycated hemoglobin ( HbAlc ) . Methods A total of 340 cases of diabetes complicated with pulmonary infection patients were enrolled for the study object by retrospective analysis from 2012 to 2015 in our hospital hospitalized in the Department of Endocrinology. It was recorded that age, sex, measurements of height, weight, waistline (WC

  11. An Overview of the 2014 ALMA Long Baseline Campaign

    CERN Document Server

    Partnership, ALMA; Vlahakis, C; Corder, S; Remijan, A; Barkats, D; Lucas, R; Hunter, T R; Brogan, C L; Asaki, Y; Matsushita, S; Dent, W R F; Hills, R E; Phillips, N; Richards, A M S; Cox, P; Amestica, R; Broguiere, D; Cotton, W; Hales, A S; Hiriart, R; Hirota, A; Hodge, J A; Impellizzeri, C M V; Kern, J; Kneissl, R; Liuzzo, E; Marcelino, N; Marson, R; Mignano, A; Nakanishi, K; Nikolic, B; Perez, J E; Pérez, L M; Toledo, I; Aladro, R; Butler, B; Cortes, J; Cortes, P; Dhawan, V; Di Francesco, J; Espada, D; Galarza, F; Garcia-Appadoo, D; Guzman-Ramirez, L; Humphreys, E M; Jung, T; Kameno, S; Laing, R A; Leon, S; Mangum, J; Marconi, G; Nagai, H; Nyman, L -A; Perley, R; Radiszcz, M; Rodón, J A; Sawada, T; Takahashi, S; Tilanus, R P J; van Kempen, T; Vilaro, B Vila; Watson, L C; Wiklind, T; Gueth, F; Tatematsu, K; Wootten, A; Castro-Carrizo, A; Chapillon, E; Dumas, G; de Gregorio-Monsalvo, I; Francke, H; Gallardo, J; Garcia, J; Gonzalez, S; Hibbard, J E; Hill, T; Kaminski, T; Karim, A; Krips, M; Kurono, Y; Lopez, C; Martin, S; Maud, L; Morales, F; Pietu, V; Plarre, K; Schieven, G; Testi, L; Videla, L; Villard, E; Whyborn, N; Zwaan, M A; Alves, F; Andreani, P; Avison, A; Barta, M; Bedosti, F; Bendo, G J; Bertoldi, F; Bethermin, M; Biggs, A; Boissier, J; Brand, J; Burkutean, S; Casasola, V; Conway, J; Cortese, L; Dabrowski, B; Davis, T A; Trigo, M Diaz; Fontani, F; Franco-Hernandez, R; Fuller, G; Madrid, R Galvan; Giannetti, A; Ginsburg, A; Graves, S F; Hatziminaoglou, E; Hogerheijde, M; Jachym, P; Serra, I Jimenez; Karlicky, M; Klaasen, P; Kraus, M; Kunneriath, D; Lagos, C; Longmore, S; Leurini, S; Maercker, M; Magnelli, B; Vidal, I Marti; Massardi, M; Maury, A; Muehle, S; Muller, S; Muxlow, T; O'Gorman, E; Paladino, R; Petry, D; Pineda, J; Randall, S; Richer, J S; Rossetti, A; Rushton, A; Rygl, K; Monge, A Sanchez; Schaaf, R; Schilke, P; Stanke, T; Schmalzl, M; Stoehr, F; Urban, S; van Kampen, E; Vlemmings, W; Wang, K; Wild, W; Yang, Y; Iguchi, S; Hasegawa, T; Saito, M; Inatani, J; Mizuno, N; Asayama, S; Kosugi, G; Morita, K -I; Chiba, K; Kawashima, S; Okumura, S K; Ohashi, N; Ogasawara, R; Sakamoto, S; Noguchi, T; Huang, Y -D; Liu, S -Y; Kemper, F; Koch, P M; Chen, M -T; Chikada, Y; Hiramatsu, M; Iono, D; Shimojo, M; Komugi, S; Kim, J; Lyo, A -R; Muller, E; Herrera, C; Miura, R E; Ueda, J; Chibueze, J; Su, Y -N; Trejo-Cruz, A; Wang, K -S; Kiuchi, H; Ukita, N; Sugimoto, M; Kawabe, R; Hayashi, M; Miyama, S; Ho, P T P; Kaifu, N; Ishiguro, M; Beasley, A J; Bhatnagar, S; Braatz, J A; Brisbin, D G; Brunetti, N; Carilli, C; Crossley, J H; D'Addario, L; Meyer, J L Donovan; Emerson, D T; Evans, A S; Fisher, P; Golap, K; Griffith, D M; Hale, A E; Halstead, D; Hardy, E J; Hatz, M C; Holdaway, M; Indebetouw, R; Jewell, P R; Kepley, A A; Kim, D -C; Lacy, M D; Leroy, A K; Liszt, H S; Lonsdale, C J; Matthews, B; McKinnon, M; Mason, B S; Moellenbrock, G; Moullet, A; Myers, S T; Ott, J; Peck, A B; Pisano, J; Radford, S J E; Randolph, W T; Venkata, U Rao; Rawlings, M; Rosen, R; Schnee, S L; Scott, K S; Sharp, N K; Sheth, K J; Simon, R S; Tsutsumi, T; Wood, S J

    2015-01-01

    A major goal of the Atacama Large Millimeter/submillimeter Array (ALMA) is to make accurate images with resolutions of tens of milliarcseconds, which at submillimeter (submm) wavelengths requires baselines up to ~15 km. To develop and test this capability, a Long Baseline Campaign (LBC) was carried out from September to late November 2014, culminating in end-to-end observations, calibrations, and imaging of selected Science Verification (SV) targets. This paper presents an overview of the campaign and its main results, including an investigation of the short-term coherence properties and systematic phase errors over the long baselines at the ALMA site, a summary of the SV targets and observations, and recommendations for science observing strategies at long baselines. Deep ALMA images of the quasar 3C138 at 97 and 241 GHz are also compared to VLA 43 GHz results, demonstrating an agreement at a level of a few percent. As a result of the extensive program of LBC testing, the highly successful SV imaging at long...

  12. Treatment decisions based on scalar and functional baseline covariates.

    Science.gov (United States)

    Ciarleglio, Adam; Petkova, Eva; Ogden, R Todd; Tarpey, Thaddeus

    2015-12-01

    The amount and complexity of patient-level data being collected in randomized-controlled trials offer both opportunities and challenges for developing personalized rules for assigning treatment for a given disease or ailment. For example, trials examining treatments for major depressive disorder are not only collecting typical baseline data such as age, gender, or scores on various tests, but also data that measure the structure and function of the brain such as images from magnetic resonance imaging (MRI), functional MRI (fMRI), or electroencephalography (EEG). These latter types of data have an inherent structure and may be considered as functional data. We propose an approach that uses baseline covariates, both scalars and functions, to aid in the selection of an optimal treatment. In addition to providing information on which treatment should be selected for a new patient, the estimated regime has the potential to provide insight into the relationship between treatment response and the set of baseline covariates. Our approach can be viewed as an extension of "advantage learning" to include both scalar and functional covariates. We describe our method and how to implement it using existing software. Empirical performance of our method is evaluated with simulated data in a variety of settings and also applied to data arising from a study of patients with major depressive disorder from whom baseline scalar covariates as well as functional data from EEG are available.

  13. Spent nuclear fuel technical baseline description, Fiscal Year 1996: Volume II, supporting data

    International Nuclear Information System (INIS)

    The Technical Baseline Description documents the Project-Level functions and requirements, along with associated enabling assumptions, issues, trade studies, interfaces, and products. It is a snapshot in time of the baseline at the beginning of September 1995. It supports the individual subprojects in the development of lower-tier functions, requirements, and specifications in FY 1996. It also supports the need for Hanford site planning to be based on an integrated Hanford site systems engineering technical baseline; and is traceable to that baseline. This document replaces and supercedes WHC-SD-SNF-SD-003

  14. HEMOGLOBIN AND HEMATOCRIT DURING AN 8 DAY MOUNTAINBIKE RACE: A FIELD STUDY

    Directory of Open Access Journals (Sweden)

    Katharina C. Wirnitzer

    2007-06-01

    Full Text Available Considering the fact that mountainbike (MTB marathon and ultraendurance events also as MTB stage races have become very popular during the last decade knowledge is sparse about the effects on hematological system due to prolonged strenuous exercise. Endurance trained athletes generally show an increased blood volume mainly due to plasma volume (PV expansion, which already exerts after a few days of prolonged exercise, accompanied by lower hemoglobin (Hb and hematocrit (Hct levels. On a short term basis dehydration caused by prolonged exertive exercise leads to enhanced concentrations of Hb and Hct due to decreased PV. In contrast to acute exercise it has been well documented as a long term adaptation that regular endurance training over long term periods or repeated bouts of strenuous exercise, e. g. repetitive cycling races or cycling stage races over several consecutive days, lead to a fall in both Hb and Hct levels due to a progressive enlargement in particular in PV. Changes in hematological parameters are known to considerably influence physical performance, especially in aerobic endurance sports such as mountainbiking. An increase in PV normally results in enhanced aerobic performance due to reduced blood viscosity, thereby optimized microcirculation and improved oxygen delivery capacity to the working muscle (Schumacher et. al., 2000. Hematological parameters Hb and Hct are highly sensible to acute effects. The effects of prolonged exercise on hematological status are mainly dependent on total load (mode and duration of exercise, as well as thermal stress (temperature and humidity and fluid intake (FI (Convertino, 1991; Fellmann et. al., 1999; Neumayr et. al., 2002; Sawka et. al., 2000; Schumacher et. al., 2000. The Transalp Challenge (TAC is one of the hardest MTB marathon races in the world (besides Cape Epic/SA and Transrockies/USA, covering eight consecutive stages. The key data of TAC 2004 are: 22. 500 m (altitude difference, 662 km

  15. The baseline dataflow system of the ATLAS trigger and DAQ

    CERN Document Server

    Vermeulen, J C; Dos Anjos, A; Barisonzi, M; Beck, H P; Beretta, M; Blair, R; Bogaerts, J A C; Boterenbrood, H; Botterill, David R; Ciobotaru, M; Palencia-Cortezon, E; Cranfield, R; Crone, G J; Dawson, J; Di Girolamo, B; Dobinson, Robert W; Ermoline, Y; Ferrer, M L; Francis, D; Gadomski, S; Gameiro, S; Golonka, P; Gorini, B; Green, B; Gruwé, M; Haas, S; Haeberli, C; Hasegawa, Y; Hauser, R; Hinkelbein, C; Hughes-Jones, R E; Jansweijer, P; Joos, M; Kaczmarska, A; Knezo, E; Kieft, G; Korcyl, K; Kugel, A; Lankford, A; Lehmann, G; Le Vine, M J; Liu, W; Maeno, T; Losada-Maia, L; Mapelli, L; Martin, B; McLaren, R; Meirosu, C; Misiejuk, A; Mommsen, R K; Mornacchi, Giuseppe; Müller, M; Nagasaka, Y; Nakayoshi, K; Papadopoulos, I M; Petersen, J; De Matos-Lopes-Pinto, P; Prigent, D; Pérez-Réale, V; Schlereth, J L; Shimojima, M; Spiwoks, R; Stancu, S; Strong, J; Tremblet, L; Werner, P; Wickens, F J; Yasu, Y; Yu, M; Zobernig, H; Zurek, M

    2003-01-01

    In this paper the baseline design of the ATLAS High Level Trigger and Data Acquisition system with respect to the DataFlow aspects, as presented in the recently submitted ATLAS Trigger/DAQ/Controls Technical Design Report [1], is reviewed and recent results of testbed measurements and from modelling are discussed. [1] ATLAS-TDR-016; CERN-LHCC-2003-022, http://cdsweb.cern.ch/search.py?recid=616089

  16. Hemoglobin, ferritin, vitamin B12 and helicobacter pylori infection: a study in patients underwent upper GI endoscopy at civil hospital karachi

    International Nuclear Information System (INIS)

    To compare hemoglobin, ferritin, and vitamin B12 levels in patients undergoing upper GI endoscopy according to their Helicobacter pylori status. All patients undergoing upper GI Endoscopy were selected, while patients of active peptic ulcers, malignancy, varices, malabsorption and recent blood donation were excluded. Blood samples for CBC, serum ferritin and vitamin B12 were collected and H.pylori status was ascertained by urease test on gastric biopsy. Differences in mean values of age, ferritin, hemoglobin, MCV and vitamin B12 were done by students t-test. Significant confounding factors were identified on multivariate analysis and were further analyzed by univariate analysis. Two hundred and eighty-five subjects were studied, including 171 males and 114 females. H-pylori was positive in 214 (75.1%) patients. Significantly low levels of hemoglobin (p =0.0), ferritin (p = 0.0) and vitamin B12 (p = 0.0) were found in patients with H-pylori infection. Gender, menopause, contraception and history of peptic ulcer disease were identified as significant confounders. Significantly low levels of hemoglobin, ferritin and vitamin B12 were found in patients with H-pylori infection. (author)

  17. Study of Hemoglobin in Vegetarian and Non-Vegetarian Diet in Obese Women with risk of cardiac Problem in Lucknow city

    Directory of Open Access Journals (Sweden)

    shyam vinay sharma

    2013-04-01

    Full Text Available Introduction: Hemoglobin level and pattern of food intake is closely associated with risk of cardiovascular diseases. The cardiovascular diseases are primarily may be due to altered lipid profile which is depend on type and pattern of food intake. Association of vegetarian and non-vegetarianisms in obese women is controversial. Method: The study was conducted on vegetarianism and non-vegetarianism obese female. A total of 55 subjects were enrolled (35 cases and 20 control for the study. The Anthropometric measurements viz. height, weight, BMI and WHR, BP, were taken and hemoglobin level (Hg % was assessed in vegetarian and non-vegetarianism obese women. Results: Our results showed that hemoglobin level (Hg % in non-vegetarianism obese women was (12.35±1.083 high than vegetarian (9.036±1.002 obese women. The blood pressure systolic and diastolic was higher in the non-vegetarian obese women than vegetarians obese female. There were no significant difference was found in heart rate in both groups i.e. vegetarianism and non-vegetarianism obese female. The Blood pressure systolic and diastolic was (127.63± 20.16 and 79.23±10.78 and (128.71± 23.14 and 83.26±9.23 in Vegetarian Non-vegetarian respectively. Discussion: Thus Hemoglobin Level (Hg % of non-vegetarianism were high in obese women than vegetarian. Due to Some nutrients intake, hemoglobin level was better in non-vegetarians than vegetarians. [Natl J Med Res 2013; 3(2.000: 169-172

  18. Free Heme and the Polymerization of Sickle Cell Hemoglobin

    OpenAIRE

    Uzunova, Veselina V.; Pan, Weichun; Galkin, Oleg; Vekilov, Peter G.

    2010-01-01

    In search of novel control parameters for the polymerization of sickle cell hemoglobin (HbS), the primary pathogenic event of sickle cell anemia, we explore the role of free heme, which may be excessively released in sickle erythrocytes. We show that the concentration of free heme in HbS solutions typically used in the laboratory is 0.02–0.04 mole heme/mole HbS. We show that dialysis of small molecules out of HbS solutions arrests HbS polymerization. The addition of 100–260 μM of free heme to...

  19. Moessbauer studies of hemoglobin in high altitude polycythemia

    International Nuclear Information System (INIS)

    The Moessbauer spectra have been measured in erythrocytes from normal adults and the patients with high altitude polycythemia (HAPC). The results indicated that two subspectra ''a'' and ''b'', corresponding to oxy- and deoxyhemoglobin respectively, were present in all blood samples, and a third subspectrum ''c'' was found to exist in almost all samples from the patients. The parameters of the third subspectra ''c1'' in most samples from the patients were similar to those of carbon monoxyhemoglobin. The components were considered to be the denatured hemoglobin in RBCs (red blood cells). Together with clinical analysis, a possible mechanism of HAPC has been discussed. (orig.)

  20. Modeling nonlinear errors in surface electromyography due to baseline noise: a new methodology.

    Science.gov (United States)

    Law, Laura Frey; Krishnan, Chandramouli; Avin, Keith

    2011-01-01

    The surface electromyographic (EMG) signal is often contaminated by some degree of baseline noise. It is customary for scientists to subtract baseline noise from the measured EMG signal prior to further analyses based on the assumption that baseline noise adds linearly to the observed EMG signal. The stochastic nature of both the baseline and EMG signal, however, may invalidate this assumption. Alternately, "true" EMG signals may be either minimally or nonlinearly affected by baseline noise. This information is particularly relevant at low contraction intensities when signal-to-noise ratios (SNR) may be lowest. Thus, the purpose of this simulation study was to investigate the influence of varying levels of baseline noise (approximately 2-40% maximum EMG amplitude) on mean EMG burst amplitude and to assess the best means to account for signal noise. The simulations indicated baseline noise had minimal effects on mean EMG activity for maximum contractions, but increased nonlinearly with increasing noise levels and decreasing signal amplitudes. Thus, the simple baseline noise subtraction resulted in substantial error when estimating mean activity during low intensity EMG bursts. Conversely, correcting EMG signal as a nonlinear function of both baseline and measured signal amplitude provided highly accurate estimates of EMG amplitude. This novel nonlinear error modeling approach has potential implications for EMG signal processing, particularly when assessing co-activation of antagonist muscles or small amplitude contractions where the SNR can be low.

  1. The mTORC1/4E-BP pathway coordinates hemoglobin production with L-leucine availability.

    Science.gov (United States)

    Chung, Jacky; Bauer, Daniel E; Ghamari, Alireza; Nizzi, Christopher P; Deck, Kathryn M; Kingsley, Paul D; Yien, Yvette Y; Huston, Nicholas C; Chen, Caiyong; Schultz, Iman J; Dalton, Arthur J; Wittig, Johannes G; Palis, James; Orkin, Stuart H; Lodish, Harvey F; Eisenstein, Richard S; Cantor, Alan B; Paw, Barry H

    2015-04-14

    In multicellular organisms, the mechanisms by which diverse cell types acquire distinct amino acids and how cellular function adapts to their availability are fundamental questions in biology. We found that increased neutral essential amino acid (NEAA) uptake was a critical component of erythropoiesis. As red blood cells matured, expression of the amino acid transporter gene Lat3 increased, which increased NEAA import. Inadequate NEAA uptake by pharmacologic inhibition or RNAi-mediated knockdown of LAT3 triggered a specific reduction in hemoglobin production in zebrafish embryos and murine erythroid cells through the mTORC1 (mammalian target of rapamycin complex 1)/4E-BP (eukaryotic translation initiation factor 4E-binding protein) pathway. CRISPR-mediated deletion of members of the 4E-BP family in murine erythroid cells rendered them resistant to mTORC1 and LAT3 inhibition and restored hemoglobin production. These results identify a developmental role for LAT3 in red blood cells and demonstrate that mTORC1 serves as a homeostatic sensor that couples hemoglobin production at the translational level to sufficient uptake of NEAAs, particularly L-leucine.

  2. The mTORC1/4E-BP pathway coordinates hemoglobin production with L-leucine availability

    Science.gov (United States)

    Chung, Jacky; Bauer, Daniel E.; Ghamari, Alireza; Nizzi, Christopher P.; Deck, Kathryn M.; Kingsley, Paul D.; Yien, Yvette Y.; Huston, Nicholas C.; Chen, Caiyong; Schultz, Iman J.; Dalton, Arthur J.; Wittig, Johannes G.; Palis, James; Orkin, Stuart H.; Lodish, Harvey F.; Eisenstein, Richard S.; Cantor, Alan B.; Paw, Barry H.

    2015-01-01

    In multicellular organisms, the mechanisms by which diverse cell types acquire distinct amino acids and how cellular function adapts to their availability are fundamental questions in biology. Here, we found that increased neutral essential amino acid (NEAA) uptake was a critical component of erythropoiesis. As red blood cells matured, expression of the amino acid transporter gene Lat3 increased, which increased NEAA import. Inadequate NEAA uptake by pharmacologic inhibition or RNAi-mediated knockdown of LAT3 triggered a specific reduction in hemoglobin production in zebrafish embryos and murine erythroid cells through the mTORC1 (mechanistic target of rapamycin complex 1)/4E-BP (eukaryotic translation initiation factor 4E-binding protein) pathway. CRISPR-mediated deletion of members of the 4E-BP family in murine erythroid cells rendered them resistant to mTORC1 and LAT3 inhibition and restored hemoglobin production. These results identify a developmental role for LAT3 in red blood cells and demonstrate that mTORC1 serves as a homeostatic sensor that couples hemoglobin production at the translational level to sufficient uptake of NEAAs, particularly L-leucine. PMID:25872869

  3. Baseline characteristics of patients with chronic kidney disease stage 3 and stage 4 in spain: the MERENA observational cohort study

    Directory of Open Access Journals (Sweden)

    Montes Rafael

    2011-10-01

    Full Text Available Abstract Background To obtain information on cardiovascular morbidity, hypertension control, anemia and mineral metabolism based on the analysis of the baseline characteristics of a large cohort of Spanish patients enrolled in an ongoing prospective, observational, multicenter study of patients with stages 3 and 4 chronic kidney diseases (CKD. Methods Multicenter study from Spanish government hospital-based Nephrology outpatient clinics involving 1129 patients with CKD stages 3 (n = 434 and 4 (n = 695 defined by GFR calculated by the MDRD formula. Additional analysis was performed with GFR calculated using the CKD-EPI and Cockcroft-Gault formula. Results In the cohort as a whole, median age 70.9 years, morbidity from all cardiovascular disease (CVD was very high (39.1%. In CKD stage 4, CVD prevalence was higher than in stage 3 (42.2 vs 35.6% p 300 mg/day was present in more than 60% of patients and there was no significant differences between stages 3 and 4 CKD (1.2 ± 1.8 and 1.3 ± 1.8 g/day, respectively. A majority of the patients had hemoglobin levels greater than 11 g/dL (91.1 and 85.5% in stages 3 and 4 CKD respectively p Conclusion This study provides an overview of key clinical parameters in patients with CKD Stages 3 and 4 where delivery or care was largely by nephrologists working in a network of hospital-based clinics of the Spanish National Healthcare System.

  4. 变异血红蛋白对糖化血红蛋白两种检测方法的干扰%Interference study of variability hemoglobin in the two detection methods of glycosylated hemoglobin

    Institute of Scientific and Technical Information of China (English)

    王淑霞

    2015-01-01

    目的:探讨变异血红蛋白对两种基于HPLC方法测定糖化血红蛋白结果的干扰。方法选取我院诊断的6种不同类型血红蛋白变异者血液及婴儿脐带血共60份作为实验组,同时选取我院体检健康者血液60份作为对照组,分别使用AC-HPLC和IE-HPLC系统对两组HbA1C进行检测,探讨7种不同变异血红蛋白对糖化血红蛋白两种检测方法的干扰并分析其原因。结果在AC-HPLC检测中,各变异血红蛋白,与对照组相比,均无明显差异,其HbA1C均与受测人员血糖浓度呈正相关。而在IE-HPLC检测中,HbD、E、G、J、Q、S均明显高于AC-HPLC和对照组中HbA1C水平,差异有统计学意义(P<0.05),但HbF变异蛋白检测中未检出HbA1C。结论 IE-HPLC系统检测糖化血红蛋白时容易受变异血红蛋白干扰,而AC-HPLC系统则不受其影响,值得推广。%Objective To study the interference of variability hemoglobin in the detection results of glycosylated hemoglobin of the two HPLC methods. Methods 60 patients with 6 kinds of different types of variability hemoglobin and infant umbilical cord blood who were diagnosed in our hospital were selected as the experimental group, while the other 60 cases of healthy blood were selected as the control group. To study and analyze the interference causes of 7 kinds of variability hemoglobin in the two detection methods.Results Of the AC-HPLC detection, all kinds of variability hemoglobin between the two groups were no significant differences, HbA1C of tested personnel was positive correlation with blood glucose concentration. HbA1C levels of HbD、E、G、J、Q、S in IE-HPLC detection were higher than which in AC-HPLC, the difference had statistical significance(P<0.05), but HbA1C wasn’t detected in HbF variability hemoglobin.Conclusion Glycosylated hemoglobin of IE-HPLC detection could easily be interfered by variability hemoglobin, while AC-HPLC detection is not affected and is

  5. Preservation of canine myocardial high-energy phosphates during low-flow ischemia with modification of hemoglobin-oxygen affinity.

    Science.gov (United States)

    Weiss, R G; Mejia, M A; Kass, D A; DiPaula, A F; Becker, L C; Gerstenblith, G; Chacko, V P

    1999-03-01

    Conventional approaches for the treatment of myocardial ischemia increase coronary blood flow or reduce myocardial demand. To determine whether a rightward shift in the hemoglobin-oxygen saturation curve would reduce the metabolic and contractile effects of a myocardial oxygen-supply imbalance, we studied the impact of a potent synthetic allosteric modifier of hemoglobin-oxygen affinity, a 2-[4-[[(3,5-disubstituted anilino)carbonyl]methyl] phenoxy] -2-methylproprionic acid derivative (RSR13), during low-flow ischemia. Changes in myocardial high-energy phosphate levels and pH were studied by 31P nuclear magnetic resonance (NMR) spectroscopy in 12 open-chest dogs randomized to receive RSR13 or vehicle control during a reversible reduction of left anterior descending (LAD) coronary artery blood flow. Changes in cardiac metabolites and regional ventricular function studied by pressure segment-length relations were also investigated in additional animals before and after RSR13 administration during low-flow LAD ischemia. The intravenous administration of RSR13 before ischemia resulted in a substantial increase in the mean hemoglobin p50 and attenuated the decline in cardiac creatine phosphate/adenosine triphosphate (PCr/ATP), percent PCr, and pH during ischemia without a change in regional myocardial blood flow, heart rate, or systolic blood pressure. RSR13 given after the onset of low-flow ischemia also improved cardiac PCr/ATP ratios and regional function as measured by fractional shortening and regional work. Thus, synthetic allosteric reduction in hemoglobin-oxygen affinity may be a new and important therapeutic strategy to ameliorate the metabolic and functional consequences of cardiac ischemia. PMID:10074492

  6. Genetic and developmental variation of hemoglobin in the deermouse, Peromyscus maniculatus.

    Science.gov (United States)

    Maybank, K M; Dawson, W D

    1976-04-01

    A genetic investigation of electrophoretic hemoglobin variants of the deermouse, Peromyscus maniculatus, shows three alleles, Hblf, Hblr, and Hblo, at a duplicated site controlling the six adult phenotypes. The Hblf allele has not been described previously. The hemoglobin locus is not closely linked to the albino locus. Fetal hemoglobin is distinct from any of the adult components and has a slower electrophoretic mobility. The fetal phenotype changes to the adult type between the days 15 and 18 of prenatal life. PMID:962849

  7. Balanced globin protein expression and heme biosynthesis improve production of human hemoglobin in Saccharomyces cerevisiae

    OpenAIRE

    Liu, Lifang; Martínez, José L.; Liu, Zihe; Petranovic, Dina; Nielsen, Jens

    2014-01-01

    Due to limitations associated with whole blood for transfusions (antigen compatibility, transmission of infections, supply and storage), the use of cell-free hemoglobin as an oxygen carrier substitute has been in the center of research interest for decades. Human hemoglobin has previously been synthesized in yeast, however the challenge is to balance the expression of the two different globin subunits, as well as the supply of the prosthetic heme required for obtaining the active hemoglobin (...

  8. Salton Sea sampling program: baseline studies

    Energy Technology Data Exchange (ETDEWEB)

    Tullis, R.E.; Carter, J.L.; Langlois, G.W.

    1981-04-13

    Baseline data are provided on three species of fish from the Salton Sea, California. The fishes considered were the orange mouth corvina (Cynoscion xanthulus), gulf croaker (Bairdiella icistius) and sargo (Anisotremus davidsonii). Morphometric and meristic data are presented as a baseline to aid in the evaluation of any physiological stress the fish may experience as a result of geothermal development. Analyses were made on muscle, liver, and bone of the fishes sampled to provide baseline data on elemental tissue burdens. The elements measured were: As, Br, Ca, Cu, Fe, Ga, K, Mn, Mi, Pb, Rb, Se, Sr, Zn, and Zr. These data are important if an environmentally sound progression of geothermal power production is to occur at the Salton Sea.

  9. Engineering the oxygen sensing regulation results in an enhanced recombinant human hemoglobin production by Saccharomyces cerevisiae

    DEFF Research Database (Denmark)

    Martínez, José L.; Liu, Lifang; Petranovic, Dina;

    2015-01-01

    engineering also allowed the generation of different genetically modified organisms for the production of recombinant human hemoglobin. Several studies have showed very promising results using the bacterium Escherichia coli as a production platform, reporting hemoglobin titers above 5% of the total cell...... the generation of a set of plasmids to produce functional human hemoglobin in Saccharomyces cerevisiae, with final titers of active hemoglobin exceeding 4% of the total cell protein. In this study, we propose a strategy for further engineering S. cerevisiae by altering the oxygen sensing pathway by deleting...

  10. The temperature dependence of refractive index of hemoglobin at the wavelengths 930 and 1100 nm

    Science.gov (United States)

    Lazareva, Ekaterina N.; Tuchin, Valery V.

    2016-04-01

    In this study, the refractive index of hemoglobin was measured at different temperatures within a physiological range and above that is characteristic to light-blood interaction at laser therapy. Measurements were carried out using the multi-wavelength Abbe refractometer (Atago, Japan). The refractive index was measured at two NIR wavelengths of 930 nm and 1100 nm. Samples of hemoglobin solutions with concentration of 80, 120 and 160 g/l were investigated. The temperature was varied between 25 and 55 °C. It was shown that the dependence of the refractive index of hemoglobin is nonlinear with temperature, which may be associated with changes in molecular structure of hemoglobin.

  11. Oxidative stress in preeclampsia an