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Sample records for based dtpa derivative

  1. Synthesis of DTPA derivatives for the separation of f elements

    International Nuclear Information System (INIS)

    During the last few years, there has been a renewal of interest in the complexation chemistry of the lanthanide and the actinide elements as it serves in vivo applications (MRI, radiotherapy) as well as supramolecular chemistry and more recently, the chemistry implicated in the back-end of nuclear fuel cycle. The design of new f-elements discriminating ligands requires a good knowledge of the factors that determine the selective complexation of the considered cations. In order to form a stable complex in aqueous solution, the ligand must possess donor atoms that satisfy the demands of the f-element. Thus, among neutral donor, the more polarizable amine nitrogens are preferred and hard anionic donors, like carboxylates, should bind to the charged centre. Such considerations lead us to design new DTPA derivatives ligands. The synthesis and the influence of the structure of the ligands on the stability constant of various f-cations complexes are presented. (authors)

  2. Introduction of new derivatives of biotin and DTPA for labeling of antibodies with 111 In ti detect malignant tumors

    International Nuclear Information System (INIS)

    Radiolabeled monoclonal antibodies, have created new innovations in diagnosis, research, and therapy of diseases in last 2 decades. One of the serious limitations of applications of radiolabeled antibodies in vivo is relatively low target to background activity. Various strategies have been proposed to solve this problem including pre-targeting methods that was suggested in 1989. Regarding importance of monoclonal antibodies and radioisotopes, based on pre-targeting strategy, we have introduced new derivative of biotin and DTPA to decrease background activity. DTPA-bio and new derivative (DTPA-bio-1 OX) were labeled with 111 In, labeled compounds and injected through tail veins into Balb/c mice, and percent of injected dose per gram of blood (% ID/g of blood ) was determined at 15, 30, 60, 120, 180 and 240 min after injection. Based on results, 111 In-DTPA-bio rapidly cleared from serum, indicating activity not bound to the target. While in the case of new derivative, by attaching 10 Adenine base (IOX) molecular weight of label is increased causing delayed clearance from serum. Therefore, there is enough time for label to accumulate in the target tissues. With advent of second generation of monoclonal antibodies and antibody engineering, pre targeting methods have changed greatly. It seems that derivatives we introduced will have and important role in new pre-targeting methods

  3. 153Sm3+ and 111In3+ DTPA derivatives with high hepatic specificity: in vivo and in vitro studies

    OpenAIRE

    Prata, M. I. M.; Santos, A.C.; Neves, M.; Geraldes, C. F. G. C.; Lima, J. J. P. de

    2002-01-01

    Two DTPA derivatives, a mono-amide derivative containing an iodinated synthon, DTPA-IOPsp (L1) and the ligand DTPA(BOM)3 (BOM=benzyloxymethyl) (L2), radiolabelled with 153Sm3+ and 111In3+, were studied as potential hepatospecific gamma scintigraphic agents. In vivo studies with Wistar rats show that the main excretory pathway for all the chelates studied is the hepatobiliary system. The complexes of L2 show even greater hepatobiliary specificity than L1, perhaps as a consequence of longer blo...

  4. MR Imaging with Gadolinium-DTPA in skull-base tumors

    International Nuclear Information System (INIS)

    Twenty-five patients were investigated by MR imaging in order to evaluate the diagnostic value of Gadolinium (Gd)-DTPA in skull-base tumors. The patients were studied with standard acquisition techniques (T1, mixed and T2-weighted images) without contrast medium. The images obtained after intravenous injection of Gd-DTPA. The contrastographic results in the different types of acquisition were evaluated. Thanks to the extra-ordinary increase in contrast resolution it provides, Gd-DTPA allowed the precise evaluation of the lesion and of its perfect spatial definition in all cases. Our experience demonstrated that Gd-DTPA considerably increases the sensitivity of the technique in this anatomical region. On the contrary, as regards the nature of the lesion, the signal did not significantly very after the iv injection of Gd-DTPA in the various kinds of lesion. In addition to the important diagnostic advantages of Gd-DTPA, its excellent tollerability and the absence of side-effects must be stressed

  5. Radiation doses deriving from patients undergoing 111In-DTPA-d-Phe-1-octreotide scintigraphy

    International Nuclear Information System (INIS)

    The purpose of this study was to estimate the radiation doses to nursing staff, other patients, accompanying persons and family members deriving from patients undergoing 111In-DTPA-d-Phe-1-octreotide (111In-OCT) scintigraphy. Dose rates were measured from 16 patients who had received an intravenous injection of 140±40 MBq 111In-OCT. The measurements were performed at three different distances (0.5, 1 and 2 m) at 10-20 min, 5-7 h and 24 h (and in some cases, up to 48 h) after administration of 111In-OCT. The effective half-lives of the biexponential decrease of the dose rates were estimated to be 2.94±0.27 h (T1) and 65.17±0.58 h (T2). The calculated maximum dose to other persons in the waiting area was 27.2 μSv, to family members 61.5 μSv, to nursing staff in a ward 24.1 μSv and to neighbouring patients in the ward 69.5 μSv. Our results clearly demonstrate that the calculated maximum radiation exposure to accompanying persons, personnel, family members and other patients is well below the maximum annual dose limit for non-professionally exposed persons. (orig.)

  6. (153)Sm(3+) and (111)In(3+) DTPA derivatives with high hepatic specificity: in vivo and in vitro studies.

    Science.gov (United States)

    Prata, M I M; Santos, A C; Neves, M; Geraldes, C F G C; de Lima, J J P

    2002-07-25

    Two DTPA derivatives, a mono-amide derivative containing an iodinated synthon, DTPA-IOPsp (L(1)) and the ligand DTPA(BOM)(3) (BOM=benzyloxymethyl) (L(2)), radiolabelled with (153)Sm(3+) and (111)In(3+), were studied as potential hepatospecific gamma scintigraphic agents. In vivo studies with Wistar rats show that the main excretory pathway for all the chelates studied is the hepatobiliary system. The complexes of L(2) show even greater hepatobiliary specificity than L(1), perhaps as a consequence of longer blood circulation times due to their strong affinity towards HSA. The (153)Sm(3+) chelates are also more hepatospecific than the corresponding (111)In(3+) chelates. The La(3+) and In(3+) chelates of L(1) and L(2) show some structural and dynamic differences in aqueous solution, as studied by (1)H NMR spectroscopy. While only two nona-coordinated isomers were observed for the La(3+) complexes with both ligands, its number is much larger in the In(3+) complexes, with both octa- and hepta-coordinated species (with unbound side arms), as well as structural isomers for each coordination number. PMID:12121790

  7. Dissolution kinetic studies of magnetite in HEEDTA and DTPA based formulation

    International Nuclear Information System (INIS)

    Magnetite is an important corrosion product accumulated on the structural materials of PHWRs and PWR's. Due to trapping of radionuclides like 6OCo, 58Co, 54Mn and activated fission products on magnetite lattice activity gets accumulated. Organic carboxylic acids can be used for the effective removal of the oxide to reduce the activity build-up. The present study details the dissolution kinetics of magnetite in hydroxyethylethylenediamine triacetic acid (HEEDTA) and diethylenetriamine-pentaacetic acid (DTPA) formulations with varying pH, temperature and reductant concentrations. The activation energy has been calculated using Arrhenius plot and was found to be 13.37 kJmol-1 and 10.37 kJmol-1 for HEEDTA and DTPA based formulations respectively. (author)

  8. Volume-assisted estimation of liver function based on Gd-EOB-DTPA-enhanced MR relaxometry

    Energy Technology Data Exchange (ETDEWEB)

    Haimerl, Michael; Schlabeck, Mona; Verloh, Niklas; Fellner, Claudia; Stroszczynski, Christian; Wiggermann, Philipp [University Hospital Regensburg, Department of Radiology, Regensburg (Germany); Zeman, Florian [University Hospital Regensburg, Center for Clinical Trials, Regensburg (Germany); Nickel, Dominik [MR Applications Development, Siemens AG, Healthcare Sector, Erlangen (Germany); Barreiros, Ana Paula [University Hospital Regensburg, Department of Internal Medicine I, Regensburg (Germany); Loss, Martin [University Hospital Regensburg, Department of Surgery, Regensburg (Germany)

    2016-04-15

    To determine whether liver function as determined by indocyanine green (ICG) clearance can be estimated quantitatively from hepatic magnetic resonance (MR) relaxometry with gadoxetic acid (Gd-EOB-DTPA). One hundred and seven patients underwent an ICG clearance test and Gd-EOB-DTPA-enhanced MRI, including MR relaxometry at 3 Tesla. A transverse 3D VIBE sequence with an inline T1 calculation was acquired prior to and 20 minutes post-Gd-EOB-DTPA administration. The reduction rate of T1 relaxation time (rrT1) between pre- and post-contrast images and the liver volume-assisted index of T1 reduction rate (LVrrT1) were evaluated. The plasma disappearance rate of ICG (ICG-PDR) was correlated with the liver volume (LV), rrT1 and LVrrT1, providing an MRI-based estimated ICG-PDR value (ICG-PDR{sub est}). Simple linear regression model showed a significant correlation of ICG-PDR with LV (r = 0.32; p = 0.001), T1{sub post} (r = 0.65; p < 0.001) and rrT1 (r = 0.86; p < 0.001). Assessment of LV and consecutive evaluation of multiple linear regression model revealed a stronger correlation of ICG-PDR with LVrrT1 (r = 0.92; p < 0.001), allowing for the calculation of ICG-PDR{sub est}. Liver function as determined using ICG-PDR can be estimated quantitatively from Gd-EOB-DTPA-enhanced MR relaxometry. Volume-assisted MR relaxometry has a stronger correlation with liver function than does MR relaxometry. (orig.)

  9. Volume-assisted estimation of liver function based on Gd-EOB-DTPA-enhanced MR relaxometry

    International Nuclear Information System (INIS)

    To determine whether liver function as determined by indocyanine green (ICG) clearance can be estimated quantitatively from hepatic magnetic resonance (MR) relaxometry with gadoxetic acid (Gd-EOB-DTPA). One hundred and seven patients underwent an ICG clearance test and Gd-EOB-DTPA-enhanced MRI, including MR relaxometry at 3 Tesla. A transverse 3D VIBE sequence with an inline T1 calculation was acquired prior to and 20 minutes post-Gd-EOB-DTPA administration. The reduction rate of T1 relaxation time (rrT1) between pre- and post-contrast images and the liver volume-assisted index of T1 reduction rate (LVrrT1) were evaluated. The plasma disappearance rate of ICG (ICG-PDR) was correlated with the liver volume (LV), rrT1 and LVrrT1, providing an MRI-based estimated ICG-PDR value (ICG-PDRest). Simple linear regression model showed a significant correlation of ICG-PDR with LV (r = 0.32; p = 0.001), T1post (r = 0.65; p < 0.001) and rrT1 (r = 0.86; p < 0.001). Assessment of LV and consecutive evaluation of multiple linear regression model revealed a stronger correlation of ICG-PDR with LVrrT1 (r = 0.92; p < 0.001), allowing for the calculation of ICG-PDRest. Liver function as determined using ICG-PDR can be estimated quantitatively from Gd-EOB-DTPA-enhanced MR relaxometry. Volume-assisted MR relaxometry has a stronger correlation with liver function than does MR relaxometry. (orig.)

  10. Additive effect of BPA and Gd-DTPA for application in accelerator-based neutron source.

    Science.gov (United States)

    Yoshida, F; Yamamoto, T; Nakai, K; Zaboronok, A; Matsumura, A

    2015-12-01

    Because of its fast metabolism gadolinium as a commercial drug was not considered to be suitable for neutron capture therapy. We studied additive effect of gadolinium and boron co-administration using colony forming assay. As a result, the survival of tumor cells with additional 5 ppm of Gd-DTPA decreased to 1/10 compared to the cells with boron only. Using gadolinium to increase the effect of BNCT instead of additional X-ray irradiation might be beneficial, as such combination complies with the short-time irradiation regimen at the accelerator-based neutron source. PMID:26242560

  11. Chitosan oligosaccharide based Gd-DTPA complex as a potential bimodal magnetic resonance imaging contrast agent.

    Science.gov (United States)

    Huang, Yan; Cao, Juan; Zhang, Qi; Lu, Zheng-rong; Hua, Ming-qing; Zhang, Xiao-yan; Gao, Hu

    2016-01-01

    A new gadolinium diethylenetriamine pentaacetic acid (DTPA) complex (Gd-DTPA-DMABA-CS11) as a potential bimodal magnetic resonance imaging (MRI) contrast agent with fluorescence was synthesized. It was synthesized by the incorporation of 4-dimethylaminobenzaldehyde (DMABA) and chitosan oligosaccharide (CSn; n=11) with low polydispersity index to DTPA anhydride and then chelated with gadolinium chloride. The structure was characterized by Fourier transform infrared (FTIR), (1)H NMR, elemental analysis and size exclusion chromatography (SEC). MRI measurements in vitro were evaluated. The results indicated that Gd-DTPA-DMABA-CS11 provided higher molar longitudinal relaxivity (r1) (12.95mM(-1)·s(-1)) than that of commercial Gd-DTPA (3.63mM(-1)·s(-1)) at 0.5T. Gd-DTPA-DMABA-CS11 also emitted fluorescence, and the intensity was much stronger than that of Gd-DTPA. Therefore, it can be meanwhile used in fluorescent imaging for improving the sensitivity in clinic diagnosis. Gd-DTPA-DMABA-CS11 as a potential contrast agent is preliminarily stable in vitro. The results of thermodynamic action between Gd-DTPA-DMABA-CS11 and bovine serum albumin (BSA) illustrated that the binding process was exothermic and spontaneous, and the main force was van der Waals' interaction and hydrogen bond. The preliminary study suggested that Gd-DTPA-DMABA-CS11 could be used in both magnetic resonance and fluorescent imaging as a promising bimodal contrast agent.

  12. Technetium-99m as alternative to produce somatostatin-labeled derivatives: comparative biodistribution evaluation with {sup 111}In-DTPA-octreotide

    Energy Technology Data Exchange (ETDEWEB)

    Melo, Ivani B.; Buchpiguel, Carlos Alberto, E-mail: ivani@hcnet.usp.br [Universidade de Sao Paulo (USP/LIM43), SP (Brazil). Centro de Medicina Nuclear. Departamento de Radiologia; Ueda, Laura T.; Araujo, Elaine B. de; Muramoto, Emiko; Barboza, Marycel F. de; Mengatti, Jair; Silva, Constancia P.G. da, E-mail: ebaraujo@ipen.com [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil). Centro de Radiofarmacia

    2008-07-01

    organs with high density of SST receptors like pancreas and adrenals were significant and similar to obtained for {sup 111}In-DTPA-Octreotide, confirming the affinity of these radiopharmaceuticals for the receptors. These results suggest that both {sup 99m}Tc-labeled SST derivatives could be applied in diagnostic studies for localization and staging of neuroendocrine tumors. (author)

  13. Synthesis, Relaxivity and MRI Enhancement of Linear Oligo-Gd(m) Complexes with Poly (DTPA-ester) Ligands Derived from Amino Acids

    Institute of Scientific and Technical Information of China (English)

    YU,Kai-Chao (俞开潮); WANG,Xin-Bing(王新兵); YE,Chao-Hui(叶朝辉); LI,Li-Yun(李丽云); LIU,Mai-Li(刘买利); ZHUO,Ren-Xi(卓仁禧)

    2001-01-01

    Six linear oligo-DrPA-ester Gd(Ⅲ) conplexes being used for potential MRI contrast agents were synthesized from amino acids and characterized. Their longitudinal relaxation rates were measured. One of them, the pheny derivative, with high rciaxivity, was chosen for the acute toxicity and T1weighted imaging test. The results indicated that there was no obvious toxicity for fhis new oligomeric Gd(Ⅲ) complex, and it exhibits the highly enhanced MRI signal intensity and the increasing signal duration in the liver tissue conpared to Gd-DTPA.

  14. Studies on Am(III) separation from simulated high-level waste using cobalt bis(dicarbollide) (1-) ion derivative covalently bound to N,N'-di-n-octyl diglycol diamide as extractant and DTPA as stripping agent

    International Nuclear Information System (INIS)

    The separation of minor actinides from high level liquid waste (HLLW) belongs to the principal challenges in current nuclear treatment. A derivative based on two cobalt bis(dicarbollide) (1-) ions covalently bound to the N,N'-di-n-octyl diglycolyl amide platform via diethyleneglycol chain with the formula ([(N,N'-(8-(OCH2 CH2)2-1,2-C2B9H10)(1',2'-C2B9H11)-3,3'-Co)(N,N'-n-C8H17)NCOCH2]2O)Na2 (TODGA-COSAN), dissolved in low polar mixture of hexyl methyl ketone and n-dodecane, was used as an extractant for efficient Am(III)/Eu(III) separation from PUREX HLLW. Am(III) could be selectively stripped from loaded organic phase by using a stripping agent composed from 0.05 M DTPA and 1 M citric acid as a buffer and 1 M NaNO3 at pH 3.0. Separation factor between europium and americium of 13 was achieved. The europium remaining in the organic phase could be consecutively effectively stripped by using solution of ammonium citrate or ammonium citrate with ammonium DTPA at pH∼7. (author)

  15. Design and synthesis of isoniazide mimetic conjugated with DTPA, potential ligand of novel radiopharmaceutical and contrast agent for medical imaging, bis (amide) of diethylenetriaminepentaacetic acid: DTPA-Bis(INH)

    International Nuclear Information System (INIS)

    The structure of isonicotinic acid hydrazide (INH) was coupled to diethylenetriaminepentaacetic acid (DTPA) via amide linkage. The overall yield of three steps synthesis starting from DTPA is above 80%. In particular, complexation of DTPA moiety with 99mTc showed excellent results as the metallopharmaceutical for medical imaging. The main objective of the present research was to develop a novel INH derivative based on DTPA, which forms stable complexes with most of the lanthanides and transition metals in the periodic table. A second objective was to introduce a chelating group without compromising the biological activity of the anti-infective drug for the diagnosis of infection using nuclear medicine. The DTPA-Bis(INH) was synthesized in high yield using a simple synthetic procedure and was characterized using mass spectroscopy in ESI positive mode ([M+H+] was found to be 632.2). Moreover, the complex with the DTPA-Bis(INH) is reflected in increased kinetic ability of the stabilized 99mTc complex compared with the unmodified INH. The complex was successfully labelled with the 99mTc radionuclide with more than 95% labelling efficiency. Radiochemical purity was ascertained chromatographically (ITLC and C18 RP chromatography) using different solvent systems. Blood kinetics in rabbits and biodistribution in mice was studied. Blood kinetics showed rapid first pass clearance with a biological half-life, t1/2 (F), of 11 min. The ability of DTPA-Bis(INH) to target the infection site in vivo was assessed in gamma scintigraphic studies of normal rabbit and a rabbit with induced infections. From the present work it can be concluded that the radiolabelled DTPABis( INH) accumulates at the site of infection. (author)

  16. Transmetallation of Gd-DTPA by Cu, Y and lanthanides and its impact on the hydrosphere

    Energy Technology Data Exchange (ETDEWEB)

    Moeller, Peter, E-mail: pemoe@gfz-potsdam.de [Helmholtz Centre Potsdam, GFZ German Research Centre for Geosciences, Telegrafenberg, 14473 Potsdam (Germany); Dulski, Peter [Helmholtz Centre Potsdam, GFZ German Research Centre for Geosciences, Telegrafenberg, 14473 Potsdam (Germany)

    2010-01-15

    The concurrent exchange of REE{sup 3+} and Y{sup 3+} (combined to M{sup 3+}) and Cu{sup 2+} for Gd{sup 3+} in Gd-DTPA (Gd-diethylenetriaminepentaacetic acid or gadopentetic acid) in the presence of clay is a very slow process if the concentrations of M{sup 3+}, Cu{sup 2+} and Gd-DTPA in solution are in the range of 0.01-22 nmol/L. The kinetics of transmetallation was followed for 1033 h without reaching equilibrium, although the release of metal ions from the clay pool is a fast process. The sum of all newly formed mono-nuclear M-DTPA species is less than the difference [Gd-DTPA{sub o}] - [Gd-DTPA] even after 1033 h but the sum of all derived M-DTPA + Cu-DTPA chelates exceeds this difference indicating that within this time span poly-nuclear chelates of Cu also formed. Formation of CuGd-DTPA chelates is the fastest process followed by formation of less stable MGd-DTPA chelates. With progress of formation of CuGd-DTPA the concentration of Gd-DTPA is lowered and consequently MGd-DTPA decomposes. Furthermore Cu{sup 2+} reacts with MGd-DTPA to form CuM-DTPA. The observed rate constants vary from species to species, whereas the pseudo-first-order-rate constants k{sub M} are nearly the same for all lanthanides. The observed rate constant for k{sub Cu} exceeds those of k{sub M} because Cu concentrations are higher than M. The changes in M speciation under the influence of DTPA are estimated for a typical composition of surface water. Input of Gd-DTPA leaves only La and, to a lesser degree, Ce unaffected by transmetallation. The total concentrations of both Cu and intermediate to heavy REE increase, whereas total Gd decreases because released Gd{sup 3+} is adsorbed by clay minerals. Depending on Cu{sup 2+} and GdL{sup 2-} concentrations in natural surface and groundwaters, Gd-DTPA decreases by about 10% within a year. Equilibrium is theoretically reached only after more than 70 a.

  17. A comparison of the rhizosphere-based method with DTPA, EDTA, CaCl2, and NaNO3 extraction methods for prediction of bioavailability of metals in soil to barley

    International Nuclear Information System (INIS)

    A rhizosphere-based method was compared with DTPA, EDTA, CaCl2, and NaNO3 extraction methods for the evaluation of bioavailability of heavy metals in soil to barley. The extractable amounts of Cr, Cu, Zn and Cd analyzed by the rhizosphere-based method correlated significantly with the metal content of barley roots. The extractable metals identified by DTPA, EDTA, CaCl2 and NaNO3 methods exhibited relatively poor or no correlation with the metal content of barley roots. The stepwise multiple regression equation of the rhizosphere-based method was the simplest one, as no soil properties needed to be entered, whereas the equations for the DTPA, EDTA, CaCl2 and NaNO3 extraction methods always require those variables. The most distinct feature of the rhizosphere-based method was that the proposed method was suitable for acidic, neutral and near alkaline soils. In contrast, the other extraction methods were restricted to soil types. In summary, the rhizosphere-based method is the most robust approach for evaluation of bioavailability of metals in soil to barley. - The rhizosphere-based method is a preferred approach for evaluating bioavailability of metals in soil

  18. Design and synthesis of isoniazide mimetic conjugated with DTPA, potential ligand of novel radiopharmaceutical and contrast agent for medical imaging, Bis (amide) of diethyelene triaminepentaacetic acid: DTPA-Bis(INH)

    International Nuclear Information System (INIS)

    The structure of isonicotinic acid hydrazide was coupled to diethylenetriaminepentaacetic acid (DTPA) via amide linkage. The Overall yield of three steps synthesis starting from DTPA is above 80%. The new ligand binds with infection site through specific interaction with gene iniA, iniB and iniC under pathological conditions. In particular, complexation of DTPA moiety with 99mTc and Gd+III showed excellent results as metallopharmaceutical for medical imaging. Primary object of the present invention is to propose a novel INH derivative based on DTPA, which form stable complexes with most of lanthanides and transition metals in periodic table. Another object of the present invention is to introduce a chelating group without compromising its biological activity for early diagnosis of infection using nuclear medicine and MR techniques. The DTPA-Bis(INH) was synthesized in high yield using simple synthetic procedure. Radiochemical purity was ascertained chromatographically using different solvent system. Blood kinetics in rabbits and biodistribution in mice was studied. The ability of DTPA-Bis(INH) to target infection site in vivo was assessed in gamma scintigraphic studies of normal rabbit and a rabbit with induced tuberculosis. The DTPA-Bis(INH) was characterized by Mass spectroscopy in ESI positive mode, M+H+ was found to be 632.2.The complex was successfully labeled with 99mTc radionuclide with more than 95% labeling efficiency.It was found stable up to 24h. Blood kinetics showed rapid first pass clearance with biological half life t1/2 (F) = 11 min. Imaging of a normal rabbit and a rabbit with induced tuberculosis was carried out. An appreciable activity was visualized in liver and kidneys. In diseased rabbit similar pattern was observed with the accumulation of activity at the tubercular site at 24 h post injection. Biodistribution revealed major accumulation in liver 6.30±0.58%ID/g at 1 h and 6.25±0.11 at 4h and in kidneys 7.65±0.52%ID/g at 1h and 6.70±0

  19. Anti-diphtheria antibody seroprotection rates are similar 10 years after vaccination with dTpa or DTPa using a mathematical model.

    Science.gov (United States)

    Cheuvart, Brigitte; Burgess, Margaret; Zepp, Fred; Mertsola, Jussi; Wolter, Joanne; Schuerman, Lode

    2004-12-01

    The reduced antigen content diphtheria, tetanus and pertussis (dTpa) vaccine (Boostrixtrade mark) has been shown to induce a strong booster response to all the vaccine components in 4-6 year olds. However, anti-diphtheria antibody levels were observed to be lower when compared to the "full strength" paediatric DTPa vaccine. To assess the impact of this difference on long-term protection, a mathematical model was developed to predict diphtheria antibody decay over time. The model was based on a linear decrease in log-transformed antibody concentrations after the first year post-vaccination. When applied to data collected 3.5 years after vaccination of 4-6 year olds with either DTPa or dTpa, the model predicted that 10 years post-vaccination, 98.6% of subjects vaccinated with dTpa were likely to remain seroprotected against diphtheria, compared to 99.6% vaccinated with DTPa. Therefore, the difference observed in diphtheria antibody geometric mean concentrations 1 month after booster vaccination at 4-6 years with dTpa or DTPa is unlikely to be of clinical relevance 10 years later at the time of the adolescent booster.

  20. Optimization of the DTPA mixed-anhydride reaction with antibodies at low concentration

    International Nuclear Information System (INIS)

    Diethylenetriaminepentaacetic acid (DTPA) was conjugated with antibody to human serum albumin (Ab) at low concentration (300 μg/ml, 2.0 μM/ via the DTPA carboxycarbonyl mixed-anhydride method. To study parameters determining the balance between the degree of conjugation and the antibody-binding activaty of Ab, a known concentration of the anhydride prepared at isobutylchloroformate (IBC)-to-DTPA ratios of 1, 2.1, or 4.2 was reacted with Ab. The percentage yields of the anhydride were determined by spectrophotometric and gravimetric titration. By the former method the percentage yields, based on DTPA concentration, were 18, 24, and 220, respectively, when the IBC-to-DTPA ratios were 1, 2.1, and 4.2. The corresponding percentage yields were 17, 39, and 262 when determined by the latter method. When the anhydride was prepared at an IBC-to-DTPA ratio of 2.1, an optimum conjugation giving three indium atoms per Ab was obtained, wtih 64% retention of antibody-binding activity. For an IBC-to-DTPA ratio of 1, the antibody retained almost 100% binding activity but the number of indium atoms incorporated (0.2) was too small. For an IBC-to-DTPA ratio of 4.2, up to 22 indium atoms were incorporated but antibody-binding activity was completely destroyed

  1. Complexation of curium(III) with DTPA at 10-70 °C: comparison with Eu(III)-DTPA in thermodynamics, luminescence, and coordination modes.

    Science.gov (United States)

    Tian, Guoxin; Zhang, Zhiyong; Martin, Leigh R; Rao, Linfeng

    2015-02-16

    Separation of trivalent actinides (An(III)) from trivalent lanthanides (Ln(III)) is a challenging task because of the nearly identical chemical properties of these groups. Diethylenetriaminepentaacetate (DTPA), a key reagent used in the TALSPEAK process that effectively separates An(III) from Ln(III), is believed to play a critical role in the An(III)/Ln(III) separation. However, the underlying principles for the separation based on the difference in the complexation of DTPA with An(III) and Ln(III) remain unclear. In this work, the complexation of DTPA with Cm(III) at 10-70 °C was investigated by spectrophotometry, luminescence spectroscopy, and microcalorimetry, in conjunction with computational methods. The binding strength, the enthalpy of complexation, the coordination modes, and the luminescence properties are compared between the Cm(III)-DTPA and Eu(III)-DTPA systems. The experimental and computational data demonstrated that the difference between Cm(III) and Eu(III) in the binding strength with DTPA can be attributed to the stronger covalence bonding between Cm(III) and the nitrogen donors of DTPA.

  2. Three-dimensional Gd-DTPA MR angiography of cerebral blood vessels

    International Nuclear Information System (INIS)

    Subtraction angiography based on three-dimensional fast MR imaging techniques using rephasing and dephasing gradient pulses is widely used. Similar angiograms can be obtained with three-dimensional gradient techniques before and after the administration of Gd-DTPA followed by imaging subtraction. As Gd-DTPA does not penetrate the blood-brain barrier, it does not alter brain images. Measurements were carried out in 14 patients with a 1.0-T whole-body imager (Siemens, Magnetom). Gd-DTPA angiography was superior to rephased-dephased angiography because of the higher signal intensity, lower noise due to short echo times, and high contrast for moving spins caused by Gd-DTPA

  3. 99mTc-DTPA dynamic SPECT and CT volumetry for measuring split renal function in live kidney donors

    International Nuclear Information System (INIS)

    )), respectively. SRF derived from DTPA/SPECT showed a better correlation with DMSA/SPECT and significantly fewer errors (P<0.05) than DTPA/P. A significant strong correlation was observed between SRF from DMSA/SPECT and CT, indicating the utility of CT renal volumetry for evaluating SRF. (author)

  4. Deriving bases for Abelian functions

    CERN Document Server

    England, Matthew

    2011-01-01

    We present a new method to explicitly define Abelian functions on algebraic curves, for the purpose of finding bases for the relevant vector spaces of such functions. We demonstrate the procedure with the functions associated to a trigonal curve of genus four. The main motivation for the construction of such bases is that it allows systematic methods for the derivation of the addition formulae and differential equations satis?ed by the functions. We present a new 3-term 2-variable addition formulae and a complete set of differential equations to generalise the classic Weierstrass identities for the case of the trigonal curve of genus four.

  5. A new radiolabelled somatostatin analogue [111In-DTPA-D-Phe1]RC-160: preparation, biological activity, receptor scintigraphy in rats and comparison with [111In-DTPA-D-Phe1]octeotide

    International Nuclear Information System (INIS)

    We have evaluated the potential usefulness of indium-111 labelled [DTPA-D-Phe1]RC-160, derived from the octapeptide somatostatin analogue RC-160, as a radiopharmaceutical for the in vivo detection of somatostatin receptor-positive tumours. For this purpose 111In- and 115In-labelled [DTPA-D-Phe1]RC-160 was tested for its biological activity, and applied for somatostatin receptor scintigraphy in vivo to rats bearing the transplantable rat pancreatic tumour CA20948, which expresses somatostatin receptors. We previously described the development of the 111In-labelled somatostatin analogue [DTPA-D-Phe1]octreotide and its use in the in vivo visualization of somatostatin receptor-positive tumours in rats and in humans. Like [111In-DTPA-D-Phe1]octreotide, [111In-DTPA-D-Phe1]RC-160 showed uptake in and specific binding in vivo to somatostatin receptor-positive organs and tumours, and the tumours were clearly visualized by gamma camera scintigraphy. However, as compared to [111In-DTPA-D-Phe1]octreotide, blood radioactivity (background) was higher, resulting in a lower tumour to blood (background) ratio. Using this animal model we therefore conclude that [111In-DTPA-D-Phe1]RC-160 has no advantage over [111In-DTPA-D-Phe1]octreotide as a radiopharmaceutical in the visualization of somatostatin receptors which bind both analogues. However, recent reports suggest the existence of different somatostatin receptor subtypes on some human cancers, which differentially bind RC-160 and not octreotide. These tumours include cancers of the breast, ovary, exocrine pancreas, prostate and colon. [111In-DTPA-D-Phe1]RC-160 might be of interest for future use in such cancer patients as a radiopharmaceutical for imaging somatostatin receptor-positive tumours, which do not bind octreotide. (orig.)

  6. Characterisation of Ho-DTPA and Y-DTPA complexes by capillary isotachophoresis and TLC with radiometric detection

    International Nuclear Information System (INIS)

    Our work was focused on the study of the Ho-DTPA and Y-DTPA complexes. Thin-layer chromatography with radiometric detection was used to establish suitable conditions for the preparation of the complexes. The optimum pH of the reaction mixture, radionuclide-to-ligand ratio, reaction time and stability of the selected complex compounds were determined. Capillary isotachophoresis (ITP) with conductivity detection was employed to determine the physico-chemical properties (stability constants) of Ho-DTPA and Y-DTPA, making use of the existence of a good linear relationship between the stability constants of lanthanide-DTPA complexes and the zone reduction due to the bleeding effect (liberation of the free metal ion). A satisfactory agreement was obtained between the Ho-DTPA and Y-DTPA stability constants determined by ITP and those retrieved from the SC-Database

  7. Financial Derivatives (Based on Two Supports Evaluation

    Directory of Open Access Journals (Sweden)

    Tiberiu Socaciu

    2011-10-01

    Full Text Available In this paper we build a PDE like Black-Scholes equation in hypothesis of a financial derivative that is dependent on two supports (usual is dependent only on one support, like am
    option based on gold, when national currency has a great float.
    Keywords: Financial derivatives, derivatives evaluation, derivatives based on two supports, extended Itō like lemma.

  8. Potential synergy between two renal toxicants: DTPA and uranium

    International Nuclear Information System (INIS)

    At present, the most appropriate therapeutic approach to treat an accidental contamination with plutonium and uranium oxide mixture (MOX) is administration of diethylene-triamine-penta-acetate acid (DTPA) in order to accelerate plutonium excretion. As uranium and DTPA are both nephro-toxic compounds, the administration of DTPA after a contamination containing uranium could enhance the nephro-toxic effects of uranium. The aim of the present work was to study in vitro on a kidney proximal tubule cell line (LLC-PK1) the cytotoxicity induced by increasing concentrations of uranium in presence of 3 different chemical forms of DTPA. The results showed that the DTPA used alone induced no cytotoxicity at the concentration used here (420 μM). However, this concentration of DTPA increased the cytotoxicity induced by uranium. This increase was maximal for uranium concentrations close to the lethal concentration for 50% of the cells and reached 37, 31 and 28% for anhydrous DTPA, Na3CaDTPA and Na3ZnDTPA, respectively. These results suggest that administration of DTPA could enhance the nephrotoxicity induced by uranium. (authors)

  9. Optimum condition for 99mTc-DTPA-ketoconazole labeling as a radiopharmaceutical for fungal infection detection

    International Nuclear Information System (INIS)

    The symptoms of infectious disease at an early stage can't be distinguished between bacterial, fungal or viral infections and often make the treatment become improper. Effective treatment, also maximum cure can be achieved if the diagnosis is accurate. In this research, drug-targeting relationship based for fungal infection diagnosis has been developed. The 99mTc-DTPA-ketoconazole radiopharmaceutical as a radiotracer was used for diagnosis of fungi infection present in the body as a target. The objective of this research is to obtain the 99mTc-DTPA-ketoconazole using indirect labeling techniques with diethylene triamine penta acetic acid (DTPA) as a co-ligand or bifunctional agent. The result showed that the optimum condition for 99mTc-DTPA-ketoconazole labeling with high radiochemical purity of 97.77±0.33% were 2 mg ketoconazole, 1.125 mg DTPA, 37.5 µg SnCl2.2H2O, pH=4.5 and incubation time at room temperature is 5 minutes. Invivo uptake test has been carried out, and the result showed high ratio of infected and non infected organ (I/NI) 2 hours post injection, that was 3.16±0.04 (n=5). From the results, it can be concluded that the optimum condition of 99mTc-DTPA-ketoconazole has been established and meets the radiochemical purity requirement as a radiopharmaceutical. (author)

  10. CanthebiliaryenhancementofGd-EOB-DTPA predictthedegreeofliverfunction?

    Institute of Scientific and Technical Information of China (English)

    Masahiro Okada; Kazunari Ishii; Kazushi Numata; Tomoko Hyodo; Seishi Kumano; Masayuki Kitano; Masatoshi Kudo; Takamichi Murakami

    2012-01-01

    BACKGROUND: Excretion of gadolinium-ethoxybenzyl-diethyl-enetriamine pentaacetic acid (Gd-EOB-DTPA) in the bile may be related to liver function, because of elimination from the liver after preferential uptake by hepatocytes. The purpose of this study was to investigate the relation between liver and biliary enhancement in patients with or without liver dysfunction, and to compare the tumor-to-liver contrast in these patients. METHODS: Forty patients [group 1: normal liver and Child-Pugh class A in 20 patients, group 2:Child-Pugh class B in 18 patients and Child-Pugh C in 2] were evaluated. All patients underwent MR imaging of the liver using a 1.5-Tesla system. T1-weighted 3D images were obtained at 5, 10, 15 and 20 minutes after Gd-EOB-DTPA injection. The relation between group 3 (total bilirubin RESULTS:Liver enhancement showed signiifcant difference at all time points between groups 1 and 2. Biliary enhancement did not show a signiifcant difference between groups 1 and 2 at 5 minutes, but did at 10, 15 and 20 minutes. At 20 minutes, signiifcant differences between groups 3 and 4 were seen for liver and biliary enhancement. At all time points, liver enhancement correlated with biliary enhancement in both groups. At 5 minutes and 20 minutes, statistical differences between groups 1 and 2 were seen for tumor-to-liver ratio. CONCLUSIONS: The degree of biliary enhancement has a close correlation to that of liver enhancement. It is especially important that insufifcient liver enhancement causes lower tumor-to-liver contrast in the hepatobiliary phase of Gd-EOB-DTPA.

  11. Determination of DTPA in urine by paper chromatography

    International Nuclear Information System (INIS)

    A paper chromatographic assay has been developed for measuring DTPA in urine to within 10% at concentrations greater than 10-5 M. This rapid method is subject to few interferences, needs only 10 to 20 μl of urine, and requires minimal handling of radioactive urine. The method has been tested on in vitro and in vivo DTPA-spiked urine samples

  12. Liver fibrosis and Gd-EOB-DTPA-enhanced MRI: A histopathologic correlation.

    Science.gov (United States)

    Verloh, Niklas; Utpatel, Kirsten; Haimerl, Michael; Zeman, Florian; Fellner, Claudia; Fichtner-Feigl, Stefan; Teufel, Andreas; Stroszczynski, Christian; Evert, Matthias; Wiggermann, Philipp

    2015-01-01

    Gadolinium ethoxybenzyl-diethylenetriaminepentaacetic acid (Gd-EOB-DTPA) is a hepatocyte-specific MRI contrast agent. Because the hepatic uptake of Gd-EOB-DTPA depends on the integrity of the hepatocyte mass, this uptake can be quantified to assess liver function. We report the relationship between the extent of Gd-EOB-DTPA uptake and the degree of liver fibrosis. T1-weighted volume-interpolated breath-hold examination (VIBE) sequences with fat suppression were acquired before and 20 minutes after contrast injection. Strong correlations of the uptake characteristics of Gd-EOB-DTPA with the relative enhancement (RE) of the liver parenchyma and the grade of fibrosis/cirrhosis, classified using the Ishak scoring system, were observed. The subdivisions between the grades of liver fibrosis based on RE were highly significant for all combinations, and a ROC revealed sensitivities ≥82% and specificities ≥87% for all combinations. MR imaging is a satisfactorily sensitive method for the assessment of liver fibrosis/cirrhosis. PMID:26478097

  13. Derivation of GFDM Based on OFDM Principles

    Energy Technology Data Exchange (ETDEWEB)

    Hussein Moradi; Behrouz Farhang-Boroujeny

    2015-06-01

    This paper starts with discussing the principle based on which the celebrated orthogonal frequency division multiplexing (OFDM) signals are constructed. It then extends the same principle to construct the newly introduced generalized frequency division multiplexing (GFDM) signals. This novel derivation sheds light on some interesting properties of GFDM. In particular, our derivation seamlessly leads to an implementation of GFDM transmitter which has significantly lower complexity than what has been reported so far. Our derivation also facilitates a trivial understanding of how GFDM (similar to OFDM) can be applied in MIMO channels.

  14. Plutonium-DTPA Model Application with USTUR Case 0269.

    Science.gov (United States)

    Konzen, Kevin; Brey, Richard; Miller, Scott

    2016-01-01

    A plutonium-DTPA (Pu-DTPA) biokinetic model was introduced that had originated from the study of a plutonium-contaminated wound. This work evaluated the extension of the Pu-DTPA model to United States Transuranium and Uranium Registry (USTUR) Case 0269 involving an acute inhalation of a plutonium nitrate aerosol. Chelation was administered intermittently for the first 7 mo as Ca-EDTA, mostly through intravenous injection, with Ca-DTPA treatments administered approximately 2.5 y post intake. Urine and fecal bioassays were collected following intake for several years. Tissues were collected and analyzed for plutonium content approximately 38 y post intake. This work employed the Pu-DTPA model for predicting the urine and fecal bioassay and final tissue quantity at autopsy. The Pu-DTPA model was integrated with two separate plutonium systemic models (i.e., ICRP Publication 67 and its proposed modification). This work illustrated that the Pu-DTPA model was useful for predicting urine and fecal bioassay, including final tissue quantity, 38 y post intake.

  15. LAT1 targeted delivery of methionine based imaging probe derived from M(III) metal ions for early diagnosis of proliferating tumours using molecular imaging modalities.

    Science.gov (United States)

    Hazari, Puja Panwar; Prakash, Surbhi; Meena, Virendra K; Jaswal, Ambika; Khurana, Harleen; Mishra, Surabhi Kirti; Bhonsle, Hemanth Kumar; Singh, Lokendra; Mishra, Anil K

    2015-01-01

    We investigated the potential of DTPA-bis(Methionine), a target specific amino acid based probe for detection of L-type amino acid transporters (LAT1) known to over express in proliferating tumours using multimodality imaging. The ligand, DTPA-bis(Met) was readily converted to lanthanide complexes and was found capable of targeting cancer cells using multimodality imaging. DTPA-bis(Met) complexes were synthesized and characterized by mass spectroscopy. MR longitudinal relaxivity, r₁ = 4.067 ± 0.31 mM⁻¹s⁻¹ and transverse relaxivity, r₂ = 8.61 ± 0.07 mM⁻¹s⁻¹ of Gd(III)-DTPA-bis(Met) were observed at pH 7.4 at 7 T. Bright, localized fluorescence of Eu(III)-DTPA-bis(Met) was observed with standard microscopy and displacement studies indicated ligand functionality. K(D) value determined for Eu(III)-DTPA-bis(Met) on U-87 MG cells was found to be 17.3 pM and showed appreciable fluorescence within the cells. Radio HPLC showed a radiochemical purity more than 95% (specific activity = 400-500 MBq/μmol, labelling efficiency 78 %) for ⁶⁸Ga(III)-DTPA-bis(Met). Pre-treatment of xenografted U-87 MG athymic mice with ⁶⁸Ga(III)-DTPA-bis(Met) following unlabelled L-methionine administration reduced tumour uptake by 10-folds in Micro PET. These data support the specific binding of ⁶⁸Ga(III)-DTPA-bis(Met) to the LAT1 transporter. To summarize, this agent possesses high stability in biological environment and exhibits effective interaction with its LAT1 transporters giving high accumulation in tumour area, excellent tumour/non-tumour ratio and low non-specific retention in vivo.

  16. Applications for Gd-DTPA in magnetic resonance imaging

    Energy Technology Data Exchange (ETDEWEB)

    Schmidt, H.; Bauer, M.; Heywang, S.; Tiling, R.; Vogl, T.; Yousry, T.

    1988-03-01

    In this article, the physics of paramagnetic contrast agents shortly will be reviewed and useful applications of Gd-DTPA in the CNS, head and neck, abdomen and pelvis will be described. In the CNS Gd-DTPA improves the sensitivity of MRI for small cerebral lesions allows a better delineation between solid tumor, oedema and necrosis. An important indication is the assessment of the extent of intramedullary tumors and the differentiation between cyst and tumor. For the diagnosis of intrameatal acoustic neuromas MRI with Gd-DTPA is the method of choice. Other useful applications are lesions of head and neck and difficult cases for mamography.

  17. Teratologic effectiveness and fetal toxicity of DTPA in the rat

    International Nuclear Information System (INIS)

    Ca-DTPA, injected intraperitoneally in rats from day 8 through 12 of gestation, was embryocidal in doses larger than 8-HD (eight times the human dose of 28.7 μmol/kg body weight). The effect was 100 percent fatal at doses greater than 16-HD. No fetal deaths occurred from Zn-DTPA, even at 64-HD. Some fetuses from dams receiving greater than 8-HD of either salt showed an increase in hydronephrosis and some alteration in kidney structure. A few minor skeletal abnormalities were also observed at these doses. In general, the teratologic effects of DTPA, if any, were minor and sporadic. (U.S.)

  18. Efficacy of liver parenchymal enhancement and liver volume to standard liver volume ratio on Gd-EOB-DTPA-enhanced MRI for estimation of liver function

    Energy Technology Data Exchange (ETDEWEB)

    Yoneyama, Tomohide; Fukukura, Yoshihiko; Kamimura, Kiyohisa; Takumi, Koji; Umanodan, Aya; Nakajo, Masayuki [Kagoshima University Graduate School of Medical and Dental Sciences, Department of Radiology, Kagoshima City (Japan); Ueno, Shinichi [Kagoshima University Graduate School of Medical and Dental Sciences, Department of Surgical Oncology and Digestive Surgery, Kagoshima City (Japan)

    2014-04-15

    We aimed to develop and assess the efficacy of a liver function index that combines liver enhancement and liver volume to standard liver volume (LV/SLV) ratio on gadolinium ethoxybenzyl diethylenetriaminepentaacetic acid (Gd-EOB-DTPA)-enhanced MRI. In all, 111 patients underwent a Gd-EOB-DTPA-enhanced MRI, including T1 mapping, before and 20 min after Gd-EOB-DTPA administration. We calculated the following Gd-EOB-DTPA-enhanced MRI-based liver function indices: relative enhancement of the liver, corrected enhancement of the liver-to-spleen ratio, LSC{sub N}20, increase rate of the liver-to-muscle ratio, reduction rate of T1 relaxation time of the liver, ΔR1 of the liver and K{sub Hep}; the indices were multiplied by the LV/SLV ratio. We calculated the correlations between an indocyanine green (ICG) clearance and the Gd-EOB-DTPA-enhanced MRI-based liver function indices multiplied by the LV/SLV ratio, by using Pearson correlation analysis. There were significant correlations between all Gd-EOB-DTPA-enhanced MRI-based liver function indices and ICG clearance (r = -0.354 to -0.574, P < 0.001). All Gd-EOB-DTPA-enhanced MRI-based liver function indices multiplied by the LV/SLV ratio (r = -0.394 to -0.700, P < 0.001) were more strongly correlated with the ICG clearance than those without multiplication by the LV/SLV ratio. Gd-EOB-DTPA-enhanced MRI-based liver function indices that combine liver enhancement and the LV/SLV ratio may more reliably estimate liver function. (orig.)

  19. Tc-99m DTPA scans in renal allograft rejection and cyclosporine nephrotoxicity

    Energy Technology Data Exchange (ETDEWEB)

    Gedroyc, W.; Taube, D.; Fogleman, I.; Neild, G.; Cameron, S.; Maisey, M.

    1986-11-01

    Renal allograft dysfunction arising from rejection or cyclosporine (CsA) nephrotoxicity can currently only be distinguished reliably by allograft biopsy. We have assessed Technetium (Tc)-99m diethylamine pentacetic acid (DTPA) scanning in 30 CsA-treated patients with allograft dysfunction. Scintigrams were performed during 20 biopsy-proved episodes of rejection and during 14 episodes of CsA nephrotoxicity. These results were compared with the scintigrams of 15 allografts showing stable function. Quantitative indices expressing allograft perfusion (flow index) and function (uptake index) derived from the DTPA scintigrams showed no significant differences between the groups of patients with rejection, CsA nephrotoxicity, or stable or improving function. Similarly, the flow and uptake indices of individual allografts obtained during periods of stable or improving function and then during episodes of dysfunction due to rejection or CsA nephrotoxicity did not significantly change. We conclude that Tc-99m DTPA scintigrams are of limited value in the management of allograft dysfunction in patients immunosuppressed with CsA.

  20. Fabrication and Characterization of Gd-DTPA-Loaded Chitosan-Poly(Acrylic Acid) Nanoparticles for Magnetic Resonance Imaging.

    Science.gov (United States)

    Ahmed, Arsalan; Zhang, Chao; Guo, Jian; Hu, Yong; Jiang, Xiqun

    2015-08-01

    Gd-DTPA-loaded chitosan-poly(acrylic acid) nanoparticles (Gd-DTPA@CS-PAA NPs) were formulated based on the reaction system of water-soluble polymer-monomer pairs of acrylic acid in chitosan solution followed by sorption of Gd-DTPA. Morphological investigations revealed the spherical shape of these NPs with about 220 nm particle size. These NPs showed charge reversal characteristic in acidic solution. In vitro and in vivo magnetic characteristics of these NPs were explored to estimate their utilization in targeted enhanced magnetic resonance imaging. Relaxation studies showed that these NPs possessed pH susceptible relaxation properties, which could introduce in vivo-specific distribution of contrast agent. MRI experiment showed that these nanoparticles had better results in contrast enhancement, and the concentration of contrast agent increased in liver and brain with increment in time. Thus, these NPs could maintain in vivo long circulation and high relaxation rate and were suitable agents for magnetic resonance imaging.

  1. Preparation of albumin microspheriods containing Zn-DTPA

    International Nuclear Information System (INIS)

    Zn-DTPA was encapsulated in materials presenting both a lipid surface (liposomes) and a protein surface. The amount of Zn-DTPA incorporated was on the order of 10-18 to 10-20 moles for a l-μm diameter capsule; barely enough to complex the Pu contained in a moderate-size Pu polymer. Neither type of microcapsule caused distress or gross lung lesions when inhaled by, or intracheally administered to rats

  2. MR-angiography with intravenous administration of Gd-DTPA

    International Nuclear Information System (INIS)

    We carried out phase contrast MR-angiography of the lower extremities with intravenous administration of Gd-DTPA. Five healthy male volunteers, 25 to 40 years of age, were examined with a 0.5T MRI unit. We used fast scan (gradient echo) technique and it took about 8 minutes for whole procedure. Images were obtained before and after intravenous injection of Gd-DTPA. Injection dose was 0.1 mmol/kg. In two cases, we got images with variable flip angles. However angles of 30 or 40 degrees were thought to be best on the scan with Gd-DTPA. In three cases, we repeated short time procedures for about 4 minutes each time and continued to check the signal intensities of vessels for as long as one hour. The signal intensities greatly increased soon after administration of Gd-DTPA, and then they gradually decreased, but for as long as 60 minutes after administration they remained much higher than those before administration of Gd-DTPA. MR-angiography with Gd-DTPA was found very useful to demonstrate the peripheral femoral vessels clearly. No significant side effect was noticed in any case. Therefore, this method was thought to be very useful clinically. (author)

  3. A feasibility study evaluating the relationship between dose and focal liver reaction in stereotactic ablative radiotherapy for liver cancer based on intensity change of Gd-EOB-DTPA-enhanced magnetic resonance images

    Energy Technology Data Exchange (ETDEWEB)

    Jung, Sang Hoon; Yu, Jeong Il; Park, Hee Chul; Lim, Do Hoon; Han, Young Yih [Dept. of Radiation Oncology, amsung Medical Center, Sungkyunkwan University School of Medicine, Seoul (Korea, Republic of)

    2016-03-15

    In order to evaluate the relationship between the dose to the liver parenchyma and focal liver reaction (FLR) after stereotactic ablative body radiotherapy (SABR), we suggest a novel method using a three-dimensional dose distribution and change in signal intensity of gadoxetate disodium-gadolinium ethoxybenzyl diethylenetriamine pentaacetic acid (Gd-EOB-DTPA)-enhanced magnetic resonance imaging (MRI) hepatobiliary phase images. In our method, change of the signal intensity between the pretreatment and follow-up hepatobiliary phase images of Gd-EOB-DTPA-enhanced MRI was calculated and then threshold dose (TD) for developing FLR was obtained from correlation of dose with the change of the signal intensity. For validation of the method, TDs for six patients, who had been treated for liver cancer with SABR with 45-60 Gy in 3 fractions, were calculated using the method, and we evaluated concordance between volume enclosed by isodose of TD by the method and volume identified as FLR by a physician. The dose to normal liver was correlated with change in signal intensity between pretreatment and follow-up MRI with a median R{sup 2} of 0.935 (range, 0.748 to 0.985). The median TD by the method was 23.5 Gy (range, 18.3 to 39.4 Gy). The median value of concordance was 84.5% (range, 44.7% to 95.9%). Our method is capable of providing a quantitative evaluation of the relationship between dose and intensity changes on follow-up MRI, as well as determining individual TD for developing FLR. We expect our method to provide better information about the individual relationship between dose and FLR in radiotherapy for liver cancer.

  4. Conversion of arterial input functions for dual pharmacokinetic modeling using Gd-DTPA/MRI and 18F-FDG/PET.

    Science.gov (United States)

    Poulin, Eric; Lebel, Réjean; Croteau, Etienne; Blanchette, Marie; Tremblay, Luc; Lecomte, Roger; Bentourkia, M'hamed; Lepage, Martin

    2013-03-01

    Reaching the full potential of magnetic resonance imaging (MRI)-positron emission tomography (PET) dual modality systems requires new methodologies in quantitative image analyses. In this study, methods are proposed to convert an arterial input function (AIF) derived from gadolinium-diethylenetriaminepentaacetic acid (Gd-DTPA) in MRI, into a (18)F-fluorodeoxyglucose ((18)F-FDG) AIF in PET, and vice versa. The AIFs from both modalities were obtained from manual blood sampling in a F98-Fisher glioblastoma rat model. They were well fitted by a convolution of a rectangular function with a biexponential clearance function. The parameters of the biexponential AIF model were found statistically different between MRI and PET. Pharmacokinetic MRI parameters such as the volume transfer constant (K(trans)), the extravascular-extracellular volume fraction (ν(e)), and the blood volume fraction (ν(p)) calculated with the Gd-DTPA AIF and the Gd-DTPA AIF converted from (18)F-FDG AIF normalized with or without blood sample were not statistically different. Similarly, the tumor metabolic rates of glucose (TMRGlc) calculated with (18) F-FDG AIF and with (18) F-FDG AIF obtained from Gd-DTPA AIF were also found not statistically different. In conclusion, only one accurate AIF would be needed for dual MRI-PET pharmacokinetic modeling in small animal models. PMID:22570280

  5. Complexation thermodynamics and structural studies of trivalent actinide and lanthanide complexes with DTPA, MS-325 and HMDTPA

    Energy Technology Data Exchange (ETDEWEB)

    Thakur, P.; Choppin, G.R. [Florida State Univ., Tallahassee, FL (United States). Dept. of Chemistry and Biochemistry; Conca, J.L. [RJ Lee Group, Inc., Pasco, WA (United States). Center for Lab. Sciences; Dodge, C.J. [Brookhaven National Laboratory, Upton, NY (United States); Francis, A.J. [Brookhaven National Laboratory, Upton, NY (United States); Pohang Univ. of Science and Technology (Korea, Republic of). Div. of Advanced Nuclear Engineering

    2013-05-01

    The protonation constants of DTPA (diethylenetriaminepentaacetic acid) and two derivatives of DTPA, 1-R(4,4-diphenyl cyclohexyl-phosphonyl-methyl diethylenentriaminepentaacetic acid) (MS-325) and (R)-hydroxymethyl-diethylenentriaminepentaacetic acid (HMDTPA) were determined by potentiometric titration in 0.1 M NaClO{sub 4}. The formation of 1: 1 complexes of Am{sup 3+}, Cm{sup 3+} and Ln{sup 3+} cations with these three ligands were investigated by potentiometric titration with competition by ethylenediaminetetraacetic acid (EDTA) and the solvent extraction method in aqueous solutions of I=0.10 M NaClO{sub 4}. The thermodynamic data of complexation were determined by the temperature dependence of the stability constants and by calorimetry. The complexation is exothermic and becomes weaker with increase in temperature. The complexation strength of these ligands follows the order: DTPA {approx} HMDTPA > MS-325. Eu{sup 3+}/Cm{sup 3+} luminescence, EXAFS (Extended X-ray Absorption Fine Structure) and DFT (Density Functional Theory) calculations suggest that all three ligands are octadentate in the complex. In the complex, M(L){sup 2-} (L = DTPA, MS-325 and HMDTPA). The M{sup 3+} binds via five carboxylates oxygen atoms, three nitrogen atoms, and the complex contains one water of hydration. (orig.)

  6. Effect of raised thoracic pressure and volume on 99mTc-DTPA clearance in humans

    Energy Technology Data Exchange (ETDEWEB)

    Nolop, K.B.; Maxwell, D.L.; Royston, D.; Hughes, J.M.

    1986-05-01

    Although positive airway pressure is often used to treat acute pulmonary edema, the effects on epithelial solute flux are not well known. We measured independently the effect of 1) positive pressure and 2) voluntary hyperinflation on the clearance of inhaled technetium-99m-labeled diethylenetriaminepentaacetic acid (/sup 99m/Tc-DTPA) in six nonsmokers and six smokers. Lung volumes were monitored by inductance plethysmography. Each subject was studied in four situations: 1) low end-expiratory volume (LO-), 2) low volume plus 9 cmH2O continuous positive airway pressure (LO+), 3) high end-expiratory volume (HI-), and 4) high volume plus continuous positive airway pressure (HI+). The clearance half time of 99mTc-DTPA for the nonsmokers decreased from 64.8 +/- 7.0 min (mean +/- SE) at LO- to 23.2 +/- 5.3 min at HI- (P less than 0.05). Positive pressure had no synergistic effect. The mean clearance half time for the smokers was faster than nonsmokers at base line but unaffected by similar changes in thoracic volume and pressure. We conclude that, in nonsmokers, positive airway pressure increases /sup 99m/Tc-DTPA clearance primarily through an increase in lung volume and that smokers are immune to these effects.

  7. Hemophilic arthropathy of the knee joint: static and dynamic Gd-DTPA - enhanced MRI

    Energy Technology Data Exchange (ETDEWEB)

    Naegele, M. [Dept. of Radiology, Univ. Bonn (Germany); Bruening, R. [Dept. of Radiology, Univ. Muenchen (Germany); Kunze, V. [Dept. of Radiology, Univ. Bonn (Germany); Eickhoff, H. [Dept. of Orthopedic Surgery, Troisdorf (Germany); Koch, W. [Dept. of Orthopedic Surgery, Troisdorf (Germany); Reiser, M. [Dept. of Radiology, Univ. Muenchen (Germany)

    1995-12-31

    A total of 17 patients with hemophilic arthropathy of the knee joint were studied with static and dynamic MRI before and after an IV bolus injection of Gadolinium-DTPA (Gd-DTPA; 0.1 mmol/kg body weight). The T1-weighted spin-echo (SE) and gradient-echo (fast-field echo [FFE]) sequences were applied. The FFE sequences of eight consecutive scans carried out over a time interval of 160 s were used in order to determine the time to signal intensity (SI) curves of the synovial proliferations surrounding soft tissue, bone marrow, and joint effusion. After the administration of a contrast agent, synovial proliferations exhibited an increase on FFE and SE images of 47.7% (SD {+-} 14.3%) and 37.4% (SD {+-} 11.2%), respectively, whereas muscle and fatty tissue, tendons, bone marrow, and joint effusion revealed only a minor increase in SI. The gradient of SI (ratio SI/time) of pannus was 39.6%/min (SD {+-} 7.7%/min) and differed significantly (P < 0.001) from that of bone marrow, fatty tissue, muscle tissue, tendons, and joint effusion (P < 0.05). In contrast to synovial proliferations in rheumatoid arthritis, no differentiation between various pannus vascularities based on the degree of enhancement was possible. The Gd-DTPA-enhanced MRI studies delineate and quantify the synovial proliferations in hemophilic arthropathy. Dynamic studies in hemophilic arthropathy do not provide qualitative assessment of the inflammatory process. (orig.)

  8. New insights into formation of trivalent actinides complexes with DTPA

    International Nuclear Information System (INIS)

    Complexation of trivalent actinides with DTPA (diethylenetriamine pentaacetic acid) was studied as a function of pcH and temperature in (Na,H)Cl medium of 0.1 M ionic strength. Formation constants of both complexes AnHDTPA- and AnDTPA2- (where An stands for Am, Cm, and Cf) were determined by TRLFS, CE-ICP-MS, spectrophotometry, and solvent extraction. The values of formation constants obtained from the different techniques are coherent and consistent with reinterpreted literature data, showing a higher stability of Cf complexes than Am and Cm complexes. The effect of temperature indicates that formation constants of protonated and non protonated complexes are exothermic with a high positive entropic contribution. DFT calculations were also performed on the An/DTPA system. Geometry optimizations were conducted on AnDTPA2- and AnHDTPA- considering all possible protonation sites. For both complexes, one and two water molecules in the first coordination sphere of curium were also considered. DFT calculations indicate that the lowest energy structures correspond to protonation on oxygen that is not involved in An-DTPA bonds and that the structures with two water molecules are not stable. (authors)

  9. Comparison of /sup 99m/Tc-DTPA and /sup 113m/In-DTPA aerosol clearances in humans. Effects of smoking, hyperinflation, and in vitro oxidation

    Energy Technology Data Exchange (ETDEWEB)

    Nolop, K.B.; Maxwell, D.L.; Fleming, J.S.; Braude, S.; Hughes, J.M.; Royston, D.

    1987-11-01

    As an index of permeability of the alveolar epithelium, the clearance of an inhaled aerosol of /sup 99m/Tc-DTPA is increased in several disease states. However, the usefulness of the test to assess the severity of disease is limited because healthy smokers also have abnormally rapid rates of clearance. Because the stability of the /sup 99m/Tc-DTPA bond might be a contributory factor, we tested the affinity of /sup 99m/Tc for DTPA in vitro, and in groups of healthy smokers (n = 13) and nonsmokers (n = 7) we measured the clearances of /sup 99m/Tc-DTPA and /sup 113m/In-DTPA, which have a similar molecular shape and charge. In vitro, sodium hypochlorite or hydrogen peroxide released as much as 98% of free /sup 99m/Tc from the /sup 99m/Tc-DTPA complex. When incubated with human neutrophils stimulated with phorbol myristate acetate, between 4 and 7% of free /sup 99m/Tc-DTPA was released after 30 min, and 12% was released after 60 min. In vivo, the clearances of both /sup 99m/Tc-DTPA and /sup 113m/In-DTPA in the smokers (n = 13) were faster than in the nonsmokers (n = 7) (p less than 0.05). Within the smokers, the mean /sup 99m/Tc-DTPA clearance (T1/2 25 +/- 4 min) was faster than the mean /sup 113m/In-DTPA clearance (34 +/- 6 min), (p less than 0.05). For nonsmokers, the difference was smaller (T1/2 /sup 99m/Tc-DTPA, 56 +/- 6; T1/2 /sup 113m/In-DTPA, 62 +/- 6) and not significant. During hyperinflation, smokers (n = 8) and nonsmokers (n = 8) both demonstrated an increase in /sup 113m/In-DTPA clearance.

  10. A novel contrast agent with rare earth-doped up-conversion luminescence and Gd-DTPA magnetic resonance properties

    International Nuclear Information System (INIS)

    The magnetic-luminescent multifunctional nanoparticles based on Gd-DTPA and NaYF4:Yb, Er were successfully synthesized by the conjugation of activated DTPA and silica-coated/surface-aminolated NaYF4:Yb, Er nanoparticles through EDC/NHS coupling chemistry. The as-prepared products were characterized by powder X-ray diffraction, transmission electron microscopy, dynamic light scattering, energy dispersive X-ray analysis, and fourier transform infrared spectrometry. The room-temperature upconversion luminescent spectra and T1-weighted maps of the obtained nanoparticles were carried out by 980 nm NIR light excitation and a 3T MR imaging scanner, respectively. The results indicated that the as-synthesized multifunctional nanoparticles with small size, highly solubility in water, and both high MR relaxivities and upconversion luminescence may have potential usage for MR imaging in future. - Graphical abstract: We have synthesized magnetic-luminescent multifunctional nanoparticles based on Gd-DTPA and NaYF4:Yb, Er by the conjugation of activated DTPA and silica-coated/surface-aminolated NaYF4:Yb, Er nanoparticles through EDC/NHS coupling chemistry. Highlights: ► A novel magnetic-luminescent multifunctional nanoparticles are synthesized. ► The nanoparticles are highly efficient for luminescence and T1-weighted MR imaging. ► The nanoparticles are small in size and highly solubility in water. ► The nanoparticles hold great potential usage for future biomedical engineering.

  11. MR lymphography with macromolecular Gd-DTPA compounds

    International Nuclear Information System (INIS)

    This paper investigates the suitability of macromolecular Gd-DTPA compounds as signal-enhancing lymphographic agents in MR imaging. Two Gd-DTPA polylysin compounds and Gd-DTPA albumin, with molecular weights of 48,000,170,000, and 87,000 daltons, respectively, were tested in rabbits at gadolinium doses of 5 and 15 μmol per animal. Three animals were examined at each dose with T1-weighted sequences. The iliac lymph nodes were imaged prior to and during unilateral endolymphatic infusion into a femoral lymph vessel as well as over a period of 2 hours thereafter. All contrast media showed a homogeneous and pronounced signal enhancement in the lymph nodes during infusion at both doses

  12. Quantification of gadolinium-DTPA concentrations for different inversion times using an IR-turbo flash pulse sequence: a study on optimizing multislice perfusion imaging

    DEFF Research Database (Denmark)

    Fritz-Hansen, T; Rostrup, Egill; Ring, P B;

    1998-01-01

    a system responding linearly to input. R1 are linearly related to changes in the concentration of gadolinium (Gd)-diethylenetriaminepentaacetic acid (DTPA), and R1 is a parameter that can be derived from the magnetic resonance (MR) signal. The accuracy of calculated R1 using an IR turbo fast low-angle shot...

  13. Comparative microvascular exchange kinetics of [{sup 77}Br]bromide and {sup 99m}Tc-DTPA in humans

    Energy Technology Data Exchange (ETDEWEB)

    Cousins, Claire [Department of Clinical Radiology, Addenbrooke' s Hospital, Cambridge (United Kingdom); Skehan, Stephen J.; Rolph, Susan M.; Flaxman, Mary E.; Ballinger, James R.; Bird, Nicholas J.; Barber, Robert W.; Peters, Michael A. [Department of Nuclear Medicine, Addenbrooke' s Hospital, Cambridge (United Kingdom)

    2002-05-01

    The plasma clearance curves of small hydrophilic solutes comprise three exponentials, consistent with a three-compartmental distribution model. A previous comparison between inulin and diethylene triamine penta-acetic acid (DTPA) suggested that these three compartments are in series, the first being plasma and the second and third representing compartments within the extravascular space. Moreover, whilst the total distribution volumes of these two indicators were similar, the volume of the second compartment was higher for DTPA. The purpose of the current study was to investigate whether a solute smaller than DTPA, namely bromide, fits the hypothesis that the second space volume is an inverse function of the size of the solute. Two groups of subjects were studied: group A comprised eight patients undergoing routine diagnostic arteriography and group B, eight patients referred for routine measurement of glomerular filtration rate plus two normal volunteers. {sup 99m}Tc-DTPA and sodium [{sup 77}Br]bromide were intravenously administered simultaneously. In group A, frequent arterial samples were obtained up to 40 min after injection, and antecubital venous samples 30 s after each arterial sample. In group B, frequent venous samples were obtained up to 280 min after injection. Volume measurements based on bromide were corrected for erythrocyte bromide accumulation. In both subject groups, the normalised venous concentration ratio of bromide to DTPA, corrected for red cell bromide uptake, was significantly less than unity in the earliest blood samples, being 0.56 (SD 0.08) at 1 min, consistent with faster diffusion of bromide from plasma to interstitial fluid. Furthermore, the extraction fraction of bromide from plasma to interstitial fluid in the forearm was about 0.6, higher than that of DTPA (about 0.5) in spite of red cell bromide accumulation which equilibrated with plasma bromide within 20 s and resulted in a red cell to plasma concentration ratio of 0.51 (0

  14. Ion-pairing HPLC methods to determine EDTA and DTPA in small molecule and biological pharmaceutical formulations

    Directory of Open Access Journals (Sweden)

    George Wang

    2016-06-01

    Full Text Available Ion-pairing high-performance liquid chromatography–ultraviolet (HPLC–UV methods were developed to determine two commonly used chelating agents, ethylenediaminetetraacetic acid (EDTA in Abilify® (a small molecule drug with aripiprazole as the active pharmaceutical ingredient oral solution and diethylenetriaminepentaacetic acid (DTPA in Yervoy® (a monoclonal antibody drug with ipilimumab as the active pharmaceutical ingredient intravenous formulation. Since the analytes, EDTA and DTPA, do not contain chromophores, transition metal ions (Cu2+, Fe3+ which generate highly stable metallocomplexes with the chelating agents were added into the sample preparation to enhance UV detection. The use of metallocomplexes with ion-pairing chromatography provides the ability to achieve the desired sensitivity and selectivity in the development of the method. Specifically, the sample preparation involving metallocomplex formation allowed sensitive UV detection. Copper was utilized for the determination of EDTA and iron was utilized for the determination of DTPA. In the case of EDTA, a gradient mobile phase separated the components of the formulation from the analyte. In the method for DTPA, the active drug substance, ipilimumab, was eluted in the void. In addition, the optimization of the concentration of the ion-pairing reagent was discussed as a means of enhancing the retention of the aminopolycarboxylic acids (APCAs including EDTA and DTPA and the specificity of the method. The analytical method development was designed based on the chromatographic properties of the analytes, the nature of the sample matrix and the intended purpose of the method. Validation data were presented for the two methods. Finally, both methods were successfully utilized in determining the fate of the chelates.

  15. Ion-pairing HPLC methods to determine EDTA and DTPA in small molecule and biological pharmaceutical formulations$

    Institute of Scientific and Technical Information of China (English)

    George Wang; Frank P. Tomasella

    2016-01-01

    Ion-pairing high-performance liquid chromatography–ultraviolet (HPLC–UV) methods were developed to determine two commonly used chelating agents, ethylenediaminetetraacetic acid (EDTA) in Abilifys (a small molecule drug with aripiprazole as the active pharmaceutical ingredient) oral solution and die-thylenetriaminepentaacetic acid (DTPA) in Yervoys (a monoclonal antibody drug with ipilimumab as the active pharmaceutical ingredient) intravenous formulation. Since the analytes, EDTA and DTPA, do not contain chromophores, transition metal ions (Cu2 þ , Fe3 þ ) which generate highly stable metallocom-plexes with the chelating agents were added into the sample preparation to enhance UV detection. The use of metallocomplexes with ion-pairing chromatography provides the ability to achieve the desired sensitivity and selectivity in the development of the method. Specifically, the sample preparation in-volving metallocomplex formation allowed sensitive UV detection. Copper was utilized for the de-termination of EDTA and iron was utilized for the determination of DTPA. In the case of EDTA, a gradient mobile phase separated the components of the formulation from the analyte. In the method for DTPA, the active drug substance, ipilimumab, was eluted in the void. In addition, the optimization of the concentration of the ion-pairing reagent was discussed as a means of enhancing the retention of the aminopolycarboxylic acids (APCAs) including EDTA and DTPA and the specificity of the method. The analytical method development was designed based on the chromatographic properties of the analytes, the nature of the sample matrix and the intended purpose of the method. Validation data were presented for the two methods. Finally, both methods were successfully utilized in determining the fate of the chelates.

  16. Optimization of the dynamic, Gd-EOB-DTPA-enhanced MRI of the liver: the effect of the injection rate

    International Nuclear Information System (INIS)

    Background Tissue-specific gadolinium-based contrast agents such as Gd-BOPTA, Gd-EOB-DTPA are increasingly used for liver imaging. Despite the added value of the hepatobiliary phase a proper arterial phase is still critical, especially in patients with chronic liver diseases. So far, there are limited data in the literature about the effect of the injection speed of Gd-EOB-DTPA in liver and vessel enhancement. Purpose To evaluate the effect of injection rate on the enhancement of liver parenchyma and vasculature in Gd-EOB-DTPA-enhanced liver MRI. Material and Methods Eighty patients who underwent Gd-EOB-DTPA-enhanced liver MRI (1.5T multi-channel MR-system) were retrospectively evaluated. We used a Care Bolus technique with an injection rate of 2 mL/s in group 1 (n = 40) and a Care Bolus technique with an injection rate of 1 mL/s in group 2 (n = 40) to determine the start of the arterial-dominant phase. Signal intensities were measured in vascular structures and liver parenchyma. Signal-to-noise-ratio (SNR), SNR increase (SNRi), and percentage enhancement (PE) were calculated and compared by a students t-test. Results The SNR, SNRi, and PE of the aorta in the arterial phase were significantly higher in group 2 in comparison to group 1 (P = 0.007, P = 0.0043, and P < 0.001, respectively). There were no significant differences concerning the SNR, SNRi, or PE of the portal vein and the normal liver parenchyma between both groups at all time points. Conclusion The study shows that a lower injection rate of 1 mL/s enables a higher enhancement in the aorta in the arterial phase compared with Gd-EOB-DTPA-enhanced MRI with the more commonly used injection rate of 2 mL/s

  17. Gadolinium-DTPA enhanced MRI in myocardial infarction

    International Nuclear Information System (INIS)

    This thesis focuses on one aspect of cardiac magnetic resonance imaging (MRI) for noninvasive screening of ischemic heart disease: the identification and quantification of acutely infarcted myocardium using gadolineum-diethylene triamine pentaacetic acid (Gd-DTPA) enhanced T1-weighted MRI in a clinical and experimental setting. (author). 296 refs.; 34 figs.; 4 tabs

  18. Real-time tracking of dissociation of hyperpolarized 89Y-DTPA: a model for degradation of open-chain Gd3+ MRI contrast agents

    Science.gov (United States)

    Ferguson, Sarah; Niedbalski, Peter; Parish, Christopher; Kiswandhi, Andhika; Kovacs, Zoltan; Lumata, Lloyd

    Gadolinium (Gd) complexes are widely used relaxation-based clinical contrast agents in magnetic resonance imaging (MRI). Gd-based MRI contrast agents with open-chain ligand such as Gd-DTPA, commercially known as magnevist, are less stable compared to Gd complexes with macrocyclic ligands such as GdDOTA (Dotarem). The dissociation of Gd-DPTA into Gd ion and DTPA ligand under certain biological conditions such as high zinc levels can potentially cause kidney damage. Since Gd is paramagnetic, direct NMR detection of the Gd-DTPA dissociation is quite challenging due to ultra-short relaxation times. In this work, we have investigated Y-DTPA as a model for Gd-DPTA dissociation under high zinc content solutions. Using dissolution dynamic nuclear polarization (DNP), the 89Y NMR signal is amplified by several thousand-fold. Due to the the relatively long T1 relaxation time of 89Y which translates to hyperpolarization lifetime of several minutes, the dissociation of Y-DTPA can be tracked in real-time by hyperpolarized 89Y NMR spectroscopy. Dissociation kinetic rates and implications on the degradation of open-chain Gd3+ MRI contrast agents will be discussed. This work was supported by the U.S. Department of Defense Award Number W81XWH-14-1-0048 and by the Robert A. Welch Foundation research Grant Number AT-1877.

  19. Toxicological study on the safety of DTPA as a drug, (1). Teratological study in the rat

    Energy Technology Data Exchange (ETDEWEB)

    Fukuda, Satoshi; Iida, Haruzo (National Inst. of Radiological Sciences, Chiba (Japan))

    1983-03-01

    In order to clarify the safety of Ca-DTPA and Zn-DTPA recommended to use as drugs in the therapeutic removal of incorporated radionuclides from the human body, the teratological study on these two agents was carried out in rats as one of a series of the toxicological tests. The teratological effects of DTPA were observed because the fetus is highly susceptible to any drug. The pregnant females of Wistar rat were injected subcutaneously daily on days 9-13 of gestation with 1, 6, 12, 24 and 36 H.D. (H.D. = human dose, 1 H.D. = 30..mu..mol/kg body weight) of Ca-DTPA or Zn-DTPA, respectively. In the dams, no toxic effects were observed. In the fetuses, the decrease of the survival rate was observed in only the group injected daily with 36 H.D. of Ca-DTPA. Some cases of gross defects of fetuses: the exencephaly, microphthalmia, anophthalmia and fusion of ribs were observed in the groups injected daily with 12, 24 and 36 H.D. of Ca-DTPA. The results obtained show that Ca-DTPA should not be given to a pregnant woman. However, no toxic effects of either Ca-DTPA or Zn-DTPA observed in the dams or of Zn-DTPA even in the fetuses indicate that these agents can be used by a radiation worker who usually is an adult man.

  20. Improved measurement of the glomerular filtration rate from Tc-99m DTPA scintigraphy in patients following nephrectomy

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Yong-il; Ha, Seunggyun [Seoul National University College of Medicine, Department of Nuclear Medicine, Seoul National University Bundang Hospital, Seongnam (Korea, Republic of); Seoul National University Bundang Hospital, Department of Nuclear Medicine, Seongnam-si, Gyeonggi-do (Korea, Republic of); So, Young [Konkuk University School of Medicine, Department of Nuclear Medicine, Chungju (Korea, Republic of); Lee, Won Woo [Seoul National University College of Medicine, Department of Nuclear Medicine, Seoul National University Bundang Hospital, Seongnam (Korea, Republic of); Seoul National University, Institute of Radiation Medicine, Medical Research Center, Seoul (Korea, Republic of); Seoul National University Bundang Hospital, Department of Nuclear Medicine, Seongnam-si, Gyeonggi-do (Korea, Republic of); Byun, Seok-Soo [Seoul National University College of Medicine, Department of Urology, Seoul National University Bundang Hospital, Seongnam (Korea, Republic of); Kim, Sang Eun [Seoul National University College of Medicine, Department of Nuclear Medicine, Seoul National University Bundang Hospital, Seongnam (Korea, Republic of); Seoul National University, Institute of Radiation Medicine, Medical Research Center, Seoul (Korea, Republic of)

    2014-02-15

    We aimed to improve Tc-99m DTPA glomerular filtration rate (GFR) scintigraphy (Gates' method) in a prospective study using Cr-51 EDTA GFR test as a gold standard. Fifty-seven Tc-99m DTPA GFR scintigrams in 45 subjects (male/female = 33:12, age = 45.9 ± 17.6 years, 14 healthy volunteers and 31 nephrectomised patients) were compared using Cr-51 EDTA GFR tests. Using the %renal uptake of Tc-99m DTPA and Cr-51 EDTA GFR, a revised equation for GFR was established through linear regression analysis. The revised equation for improved GFR was GFR(mL/min) = (%renal uptake x 11.7773) - 0.7354. Gates' original GFRs (70.1 ± 20.5 mL/min/1.73 m{sup 2}) were significantly lower than Cr-51 EDTA GFRs (97.0 ± 31.9 mL/min/1.73 m{sup 2}; P < 0.0001), but the improved GFRs (98.0 ± 26.3 mL/min/1.73 m{sup 2}) were not different from (P = 0.7360) and had a significant correlation with (r = 0.73, P < 0.0001) the Cr-51 EDTA GFRs. The revised GFR equation effectively demonstrated perioperative GFR changes in kidneys that were operated on and the contralateral kidneys at 3 and 6 months post-partial nephrectomy (n = 25). GFR measurement using Tc-99m DTPA scintigraphy could be significantly improved by a revised equation derived from the comparison with Cr-51 EDTA GFR. (orig.)

  1. Gd-DTPA Adsorption on Chitosan/Magnetite Nanocomposites.

    Science.gov (United States)

    Pylypchuk, Ie V; Kołodyńska, D; Kozioł, M; Gorbyk, P P

    2016-12-01

    The synthesis of the chitosan/magnetite nanocomposites is presented. Composites were prepared by co-precipitation of iron(II) and iron(III) salts by aqueous ammonia in the 0.1 % chitosan solution. It was shown that magnetite synthesis in the chitosan medium does not affect the magnetite crystal structure. The thermal analysis data showed 4.6 % of mass concentration of chitosan in the hybrid chitosan/magnetite composite. In the concentration range of initial Gd-DTPA solution up to 0.4 mmol/L, addition of chitosan to magnetite increases the adsorption capacity and affinity to Gd-DTPA complex. The Langmuir and Freundlich adsorption models were applied to describe adsorption processes. Nanocomposites were characterized by scanning electron microscopy (SEM), differential thermal analysis (DTA), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), and specific surface area determination (ASAP) methods. PMID:27030468

  2. Gd-DTPA Adsorption on Chitosan/Magnetite Nanocomposites

    Science.gov (United States)

    Pylypchuk, Ie. V.; Kołodyńska, D.; Kozioł, M.; Gorbyk, P. P.

    2016-03-01

    The synthesis of the chitosan/magnetite nanocomposites is presented. Composites were prepared by co-precipitation of iron(II) and iron(III) salts by aqueous ammonia in the 0.1 % chitosan solution. It was shown that magnetite synthesis in the chitosan medium does not affect the magnetite crystal structure. The thermal analysis data showed 4.6 % of mass concentration of chitosan in the hybrid chitosan/magnetite composite. In the concentration range of initial Gd-DTPA solution up to 0.4 mmol/L, addition of chitosan to magnetite increases the adsorption capacity and affinity to Gd-DTPA complex. The Langmuir and Freundlich adsorption models were applied to describe adsorption processes. Nanocomposites were characterized by scanning electron microscopy (SEM), differential thermal analysis (DTA), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), and specific surface area determination (ASAP) methods.

  3. Relationship of end-expiratory pressure, lung volume, and /sup 99m/Tc-DTPA clearance

    International Nuclear Information System (INIS)

    We investigated the dose-response effect of positive end-expiratory pressure (PEEP) and increased lung volume on the pulmonary clearance rate of aerosolized technetium-99m-labeled diethylenetriaminepentaacetic acid (99mTc-DTPA). Clearance of lung radioactivity was expressed as percent decrease per minute. Base-line clearance was measured while anesthetized sheep (n = 20) were ventilated with 0 cmH2O end-expiratory pressure. Clearance was remeasured during ventilation at 2.5, 5, 10, 15, or 20 cmH2O PEEP. Further studies showed stepwise increases in functional residual capacity (FRC) (P less than 0.05) measured at 0, 2.5, 5, 10, 15, and 20 cmH2O PEEP. At 2.5 cmH2O PEEP, the clearance rate was not different from that at base line (P less than 0.05), although FRC was increased from base line. Clearance rate increased progressively with increasing PEEP at 5, 10, and 15 cmH2O (P less than 0.05). Between 15 and 20 cmH2O PEEP, clearance rate was again unchanged, despite an increase in FRC. The pulmonary clearance of aerosolized /sup 99m/Tc-DTPA shows a sigmoidal response to increasing FRC and PEEP, having both threshold and maximal effects. This relationship is most consistent with the hypothesis that alveolar epithelial permeability is increased by lung inflation

  4. In vivo metabolite compartmentalization probed using intracellular GdDTPA

    DEFF Research Database (Denmark)

    Peters, David Alberg; Rowland, Ian

    Fast trans-membrane water exchange enables in- tracellular relaxation enhancement of water by contrast agents in the extracellular space. For me- tabolites not in fast exchange across membranes, intracellular metabolite relaxation enhancement will only occur if the contrast agent and metabolite a...... are in the same compartment. Extracellular contrast has utilized electroporation methods to deliver GdDTPA into the cytosol of rat muscle in vivo in order to probe the intracellular compart- mentalization of MR-visible metabolites....

  5. Pricing Model of Multiattribute Derivatives Based on Mixed Process

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    By Analyzing the behavior and character of derivative security, the authorsestablished a pricing model of multiattribute derivative security whose underlying asset pricingprocess is a mixed process, and obtained a new model for option pricing of multiattribute derivatives based on mixed process, and improved some original results.

  6. Toxicity of inhaled Ca-DTPA in the beagle dog

    International Nuclear Information System (INIS)

    There are several advantages to the administration of Ca- DTPA by inhalation rather than intravenous drip for the decorporation of certain radionuclides. Among these are the possibility of treating very promptly following an accidental incorporation to achieve maximum treatment effectiveness and convenince for medical management, even to the extent that treatment can be self-administered. The present investigational New Drug permit allows treatment of humans only by the intravenous route and animal studies are required to justify the new route. Earlier work in rats and hamsters showed five successive daily inhalations of Ca-DTPA aerosols (dose 1 to 4 times human i.v. dose) produced a transitory emphysema in 17/40 rats serially sacrificed up to 3 weeks following the last exposure and in 10/20 hamsters up to 1 week after exposure. No emphysema was seen in rats sacrificed after 3 weeks and in hamsters after 1 week following the exposures. Results of tests in dogs administered DTPA by inhalation showed hyperplasia of the gastric submucosal lymphoid follicles observed 1 week following the last exposure may be treatment-related, but other observed changes were considered unrelated. (U.S.)

  7. Spectroscopic studies on interaction of BSA and Eu(III) complexes with H5ph-dtpa and H5dtpa ligands.

    Science.gov (United States)

    Kong, Deyong; Qin, Cui; Fan, Ping; Li, Bing; Wang, Jun

    2015-04-01

    An novel aromatic aminopolycarboxylic acid ligand, N-(2-N,N-Dicarboxymethylaminophenyl) ethylenediamine-N,N',N'-triacetic acid (H5ph-dtpa), was synthesized by improving experimental method and its corresponding Eu(III) complex, Na2[EuIII(ph-dtpa)(H2O)]·6H2O, was successfully prepared through heat-refluxing method. As a comparison, the Eu(III) complex with diethylenetriamine-N,N,N',N',N″-pentaacetic acid (H5dtpa) ligand, Na2[Eu(III)(dtpa)(H2O)]·6H2O, was also prepared by the same method. And then, the interaction between prepared Eu(III) complexes ([EuIII(dtpa)(H2O)]2- and [EuIII(ph-dtpa)(H2O)]2-) and bovine serum albumin (BSA) in aqueous solution were studied by the combination of ultraviolet-visible (UV-vis), fluorescence and circular dichroism (CD) spectroscopies. In addition, the binding sites of Eu(III) complexes ([EuIII(dtpa)(H2O)]2- and [EuIII(ph-dtpa)(H2O)]2-) to BSA molecules were also estimated by synchronous fluorescence. Moreover, the theoretical and experimental results show that the Van der Waals, hydrogen bond and π-π stacking interactions are the mainly impulse to the reaction. The binding distances (r) between Eu(III) complexes ([EuIII(dtpa)(H2O)]2- and [EuIII(ph-dtpa)(H2O)]2-) and BSA were obtained according to Förster's non-radiative energy transfer theory. Also, the determined UV-vis absorption spectroscopy, synchronous fluorescence and circular dichroism (CD) spectra showed that the conformation of BSA could be changed in the presence of Eu(III) complexes. The obtained results can help understand the action mode between rare earth metal complexes of aminopolycarboxylic acid ligands with BSA and they are also expected to provide important information of designs of new inspired drugs.

  8. Analysis of pharmacokinetics of Gd-DTPA for dynamic contrast-enhanced magnetic resonance imaging.

    Science.gov (United States)

    Taheri, Saeid; Shah, N Jon; Rosenberg, Gary A

    2016-09-01

    The pharmacokinetics (PK) of the contrast agent Gd-DTPA administered intravenously (i.v.) for contrast-enhanced MR imaging (DCE-MRI) is an important factor for quantitative data acquisition. We studied the effect of various initial bolus doses on the PK of Gd-DTPA and analyzed population PK of a lower dose for intra-subject variations in DCE-MRI. First, fifteen subjects (23-85years, M/F) were randomly divided into four groups for DCE-MRI with different Gd-DTPA dose: group-I, 0.1mmol/kg, n=4; group-II, 0.05mmol/kg, n=4; group-III, 0.025mmol/kg, n=4; and group-IV, 0.0125mmol/kg, n=3. Sequential fast T1 mapping sequence, after a bolus i.v. Gd-DTPA administered, and a linear T1-[Gd-DTPA] relationship were used to estimate the PK of Gd-DTPA. Secondly, MR-acquired PKs of Gd-DTPA from 58 subjects (28-80years, M/F) were collected retrospectively, from an ongoing study of the brain using DCE-MRI with Gd-DTPA at 0.025mmol/kg, to statistically analyze population PK of Gd-DTPA. We found that the PK of Gd-DTPA (i.v. 0.025mmol/kg) had a half-life of 37.3±6.6min, and was a better fit into a linear T1-[Gd-DTPA] relationship than higher doses (up to 0.1mmol/kg). The area under the curve (AUC) for 0.025mmol/kg was 3.37±0.46, which was a quarter of AUC of 0.1mmol/kg. In population analysis, a dose of 0.025mmol/kg of Gd-DTPA provided less than 5% subject-dependent variation in the PK of Gd-DTPA. Administration of 0.025mmol/kg Gd-DTPA enabled us to estimate [Gd-DTPA] from T1 by using a linear relationship that has a lower estimation error compared to a non-linear relationship. DCE-MRI with a quarter dose of Gd-DTPA is more sensitive to detect changes in [Gd-DTPA].

  9. Analysis of pharmacokinetics of Gd-DTPA for dynamic contrast-enhanced magnetic resonance imaging.

    Science.gov (United States)

    Taheri, Saeid; Shah, N Jon; Rosenberg, Gary A

    2016-09-01

    The pharmacokinetics (PK) of the contrast agent Gd-DTPA administered intravenously (i.v.) for contrast-enhanced MR imaging (DCE-MRI) is an important factor for quantitative data acquisition. We studied the effect of various initial bolus doses on the PK of Gd-DTPA and analyzed population PK of a lower dose for intra-subject variations in DCE-MRI. First, fifteen subjects (23-85years, M/F) were randomly divided into four groups for DCE-MRI with different Gd-DTPA dose: group-I, 0.1mmol/kg, n=4; group-II, 0.05mmol/kg, n=4; group-III, 0.025mmol/kg, n=4; and group-IV, 0.0125mmol/kg, n=3. Sequential fast T1 mapping sequence, after a bolus i.v. Gd-DTPA administered, and a linear T1-[Gd-DTPA] relationship were used to estimate the PK of Gd-DTPA. Secondly, MR-acquired PKs of Gd-DTPA from 58 subjects (28-80years, M/F) were collected retrospectively, from an ongoing study of the brain using DCE-MRI with Gd-DTPA at 0.025mmol/kg, to statistically analyze population PK of Gd-DTPA. We found that the PK of Gd-DTPA (i.v. 0.025mmol/kg) had a half-life of 37.3±6.6min, and was a better fit into a linear T1-[Gd-DTPA] relationship than higher doses (up to 0.1mmol/kg). The area under the curve (AUC) for 0.025mmol/kg was 3.37±0.46, which was a quarter of AUC of 0.1mmol/kg. In population analysis, a dose of 0.025mmol/kg of Gd-DTPA provided less than 5% subject-dependent variation in the PK of Gd-DTPA. Administration of 0.025mmol/kg Gd-DTPA enabled us to estimate [Gd-DTPA] from T1 by using a linear relationship that has a lower estimation error compared to a non-linear relationship. DCE-MRI with a quarter dose of Gd-DTPA is more sensitive to detect changes in [Gd-DTPA]. PMID:27109487

  10. Photocatalytic Based Degradation Processes of Lignin Derivatives

    Directory of Open Access Journals (Sweden)

    Colin Awungacha Lekelefac

    2015-01-01

    Full Text Available Photocatalysis, belonging to the advanced oxidation processes (AOPs, is a potential new transformation technology for lignin derivatives to value added products (e.g., phenol, benzene, toluene, and xylene. Moreover, lignin represents the only viable source to produce aromatic compounds as fossil fuel alternative. This review covers recent advancement made in the photochemical transformation of industrial lignins. It starts with the photochemical reaction principle followed by results obtained by varying process parameters. In this context, influences of photocatalysts, metal ions, additives, lignin concentration, and illumination intensity and the influence of pH are presented and discussed. Furthermore, an overview is given on several used process analytical methods describing the results obtained from the degradation of lignin derivatives. Finally, a promising concept by coupling photocatalysis with a consecutive biocatalytic process was briefly reviewed.

  11. A novel contrast agent with rare earth-doped up-conversion luminescence and Gd-DTPA magnetic resonance properties

    Science.gov (United States)

    Lu, Qing; Wei, Daixu; Cheng, Jiejun; Xu, Jianrong; Zhu, Jun

    2012-08-01

    The magnetic-luminescent multifunctional nanoparticles based on Gd-DTPA and NaYF4:Yb, Er were successfully synthesized by the conjugation of activated DTPA and silica-coated/surface-aminolated NaYF4:Yb, Er nanoparticles through EDC/NHS coupling chemistry. The as-prepared products were characterized by powder X-ray diffraction, transmission electron microscopy, dynamic light scattering, energy dispersive X-ray analysis, and fourier transform infrared spectrometry. The room-temperature upconversion luminescent spectra and T1-weighted maps of the obtained nanoparticles were carried out by 980 nm NIR light excitation and a 3T MR imaging scanner, respectively. The results indicated that the as-synthesized multifunctional nanoparticles with small size, highly solubility in water, and both high MR relaxivities and upconversion luminescence may have potential usage for MR imaging in future.

  12. A novel contrast agent with rare earth-doped up-conversion luminescence and Gd-DTPA magnetic resonance properties

    Energy Technology Data Exchange (ETDEWEB)

    Lu Qing [Department of Radiology, Shanghai Renji Hospital, Shanghai Jiao Tong University School of Medicine, 1630 Dong Fang Rd, Shanghai 200127 (China); Wei Daixu [National Engineering Research Center for Nanotechnology, 28 East Jiang Chuan Rd, Shanghai 200241 (China); Cheng Jiejun [Department of Radiology, Shanghai Renji Hospital, Shanghai Jiao Tong University School of Medicine, 1630 Dong Fang Rd, Shanghai 200127 (China); Xu Jianrong, E-mail: xujianr@hotmail.com [Department of Radiology, Shanghai Renji Hospital, Shanghai Jiao Tong University School of Medicine, 1630 Dong Fang Rd, Shanghai 200127 (China); Zhu Jun, E-mail: yzjzhu@163.com [National Engineering Research Center for Nanotechnology, 28 East Jiang Chuan Rd, Shanghai 200241 (China)

    2012-08-15

    The magnetic-luminescent multifunctional nanoparticles based on Gd-DTPA and NaYF{sub 4}:Yb, Er were successfully synthesized by the conjugation of activated DTPA and silica-coated/surface-aminolated NaYF{sub 4}:Yb, Er nanoparticles through EDC/NHS coupling chemistry. The as-prepared products were characterized by powder X-ray diffraction, transmission electron microscopy, dynamic light scattering, energy dispersive X-ray analysis, and fourier transform infrared spectrometry. The room-temperature upconversion luminescent spectra and T{sub 1}-weighted maps of the obtained nanoparticles were carried out by 980 nm NIR light excitation and a 3T MR imaging scanner, respectively. The results indicated that the as-synthesized multifunctional nanoparticles with small size, highly solubility in water, and both high MR relaxivities and upconversion luminescence may have potential usage for MR imaging in future. - Graphical abstract: We have synthesized magnetic-luminescent multifunctional nanoparticles based on Gd-DTPA and NaYF4:Yb, Er by the conjugation of activated DTPA and silica-coated/surface-aminolated NaYF4:Yb, Er nanoparticles through EDC/NHS coupling chemistry. Highlights: Black-Right-Pointing-Pointer A novel magnetic-luminescent multifunctional nanoparticles are synthesized. Black-Right-Pointing-Pointer The nanoparticles are highly efficient for luminescence and T{sub 1}-weighted MR imaging. Black-Right-Pointing-Pointer The nanoparticles are small in size and highly solubility in water. Black-Right-Pointing-Pointer The nanoparticles hold great potential usage for future biomedical engineering.

  13. Actinides exposure: review of Ca-DTPA injections inside Cea-Cogema plants; Exposition aux actinides: bilan des injections de Ca-DTPA dans les centres CEA-Cogema

    Energy Technology Data Exchange (ETDEWEB)

    Grappin, L.; Berard, P.; Beau, P.; Carbone, L.; Castagnet, X.; Courtay, C.; Le Goff, J.P.; Menetrier, F.; Neron, M.; Piechowski, J. [CEA Cadarache, Dir. de l' Energie Nucleaire, Dept. de Soutien en surete et securite, Sev. de Sante au Travail, 13 - Saint-Paul-lez-Durance (France)

    2006-07-01

    Ca-DTPA has been used for medical treatment of plutonium and americium contaminations in the CEA and COGEMA plants from 1970 to 2003. This report is a survey of the injections administered of Ca-DTPA as a chelating molecule. This report will be a part of the AMM process for Ca-DTPA by intravenous administration submitted by the Central Pharmacy of the french Army. Out of 1158 injections administered to 469 persons, 548 events of possible or confirmed contaminations were reported. These employees were followed by occupational physicians according to the current regulations. The first part of the report is a synthesis of the most recent findings. Due to its short biological period and its limited action in the blood, Ca-DTPA does not chelate with plutonium and americium as soon as these elements are deposited in the target organs. It justifies an early treatment, even in cases of suspected contamination followed by additional injections if necessary. The second part presents data concerning these 1158 injections (way of contamination, posology, adverse effects...). These incidents took place at work, were most often minor, not requiring follow-up treatment. A study concerning the effectiveness of the product was done on a group of people having received 5 or more injections. These results were compared with effectiveness estimated from theoretical basis. Posologies and therapeutic schemes were proposed based on these observations. Additional studies are needed to confirm these findings. This document is the first synthesis in this field. It is the result of a collective work having mobilized the occupational medicine departments, the laboratories of CEA and COGEMA and a working group CEA-COGEMA-SPRA. (authors)

  14. Potential Opponent for 18FDG: Gd3+-DTPA-DG: A New Synthetic MRI Contrast Agent

    Directory of Open Access Journals (Sweden)

    Mehdi Shafiee Ardestani

    2010-05-01

    Full Text Available Glucose mediated imaging tools have recently be-come an area of research interest in the field of nuclear medicine, especially Positron Emission Tomography (PET imaging. The difficulties in the use, preparation and cost of radioactively-labeled glycosylated compounds led to the research and development in this present study of a new gadolinium-labeled glucose that does not have a radioactive half-life or difficulties in its synthesis and utilization. Based on the structure of fluorodeoxyglucose (18FDG, a new compound consisting of a D-glucose conjugated to a well-known chelator DTPA was synthesized, labeled with Gd3+ and examined in vitro and in vivo. The results showed a good anti-cancer potency for the prepared complex. Gd3+ -DTPA-DG did not produce any significant alteration in the blood glucose level and was also phosphorylated by hexokinase enzyme. In vitro relaxation times T1 and T2 were determined and compared with a standard compound Magnevist®. Finally, imaging studies were performed in tumor-bearing mice and the tumor areas were shown successfully. This compound may have great potential in the future in radio-oncology.

  15. An analysis of 99mTc-DTPA renal dynamic imaging in patients with diabetes mellitus

    International Nuclear Information System (INIS)

    To investigate the regularity of renal dynamic imaging in the various courses of diabetes mellitus, and then to provide the important evidence variety for diagnosis and treatment. The data of renal dynamic imaging was analyzed on 276 kidneys of 40 normal and 98 diabetic patients, and according to course of disease, the diabetic patients was divided into three groups: ≤1 year, 1-5 year and ≥5 year respectively. Based upon was grouped 99mTc-DTPA renal dynamic imaging and others, the renal functions into normal, approximately normal, I, II and III injury respectively, and the statistics and analysis of these data were carried out. The extent of renal function injury was related to the course of disease significantly, and it showed that the longer the course, the heavier the damage of renal function, and there was a significant difference between them (P99mTc-DTPA renal dynamic imaging can effectively evaluate the extent of renal functions injury and was also valuable for the clinical. Also, the study indicated that in diabetic patients susceptible to urinary tract infection could aggravate the injury of renal function

  16. The use of EDTA and DTPA for accelerating the removal of deposited transuranic elements from humans

    CERN Document Server

    Spoor, N L

    1977-01-01

    EDTA and DTPA have been prominent among the chelating agents used to increase the rate of excretion of certain deposited heavy metals from the human body. Since 1959, DTPA, administered either by intravenous injection or by aerosol inhalation, has been widely used to treat workers contaminated by plutonium or a higher actinide. In this report, an attempt is made to assess the toxicities of EDTA and DTPA and to evaluate the effectiveness and safety of DTPA as a drug for removing deposited transuranic elements.

  17. Fractional Diffusion based on Riemann-Liouville Fractional Derivatives

    OpenAIRE

    Hilfer, R.

    2000-01-01

    A fractional diffusion equation based on Riemann-Liouville fractional derivatives is solved exactly. The initial values are given as fractional integrals. The solution is obtained in terms of $H$-functions. It differs from the known solution of fractional diffusion equations based on fractional integrals. The solution of fractional diffusion based on a Riemann-Liouville fractional time derivative does not admit a probabilistic interpretation in contrast with fractional diffusion based on frac...

  18. Assessing liver function by liver enhancement during the hepatobiliary phase with Gd-EOB-DTPA-enhanced MRI at 3 Tesla

    Energy Technology Data Exchange (ETDEWEB)

    Verloh, N.; Haimerl, M.; Schlabeck, M.; Schreyer, A.G.; Stroszczynski, C.; Fellner, C.; Wiggermann, P. [University Hospital Regensburg, Department of Radiology, Regensburg (Germany); Zeman, F. [University Hospital Regensburg, Center for Clinical Trials, Regensburg (Germany); Barreiros, A. [University Hospital Regensburg, Department of Gastroenterology, Regensburg (Germany); Loss, M. [University Hospital Regensburg, Department of Surgery, Regensburg (Germany)

    2014-05-15

    The purpose of this study was to evaluate the usefulness of Gd-EOB-DTPA-enhanced 3-T MRI to determine the hepatic functional reserve expressed by the model for end-stage liver disease (MELD) score. A total of 121 patients with normal liver function (NLF; MELD score ≤ 10) and 29 patients with impaired liver function (ILF; MELD score > 10) underwent contrast-enhanced MRI with a hepatocyte-specific contrast agent at 3T. T1-weighted volume interpolated breath-hold examination (VIBE) sequences with fat suppression were acquired before and 20 min after contrast injection. Relative enhancement (RE) between plain signal intensity and contrast-enhanced signal intensity was calculated and was used to determine Gd-EOB-DTPA uptake into the liver parenchyma for patients with different MELD scores. RE differed significantly (p ≤ 0.001) between patients with NLF (87.2 ± 29.5 %) and patients with ILF (45.4 ± 26.5 %). The optimal cut-off value for RE to differentiate NLF from ILF was 47.7 % (AUC 0.87). This cut-off value showed a sensitivity of 82.8 % and a specificity of 92.7 % for the differentiation of the analysed groups. Gd-EOB-DTPA uptake in hepatocytes is strongly affected by liver function. Gd-EOB-DTPA-enhanced MRI and assessment of RE during the hepatobiliary phase (HBP) may serve as a useful image-based test in liver imaging for determining regional and global liver function. (orig.)

  19. Sup(99m)Tc-DTPA radio aerosol ventilation study

    International Nuclear Information System (INIS)

    Papers on deposition pattern analyses of radioaerosol lung scans are few and not easy to interpret as there is a general lack of technical standardization and the number of patients studied is not large. Moreover these reports have been generally obtained with non-hydrosoluble radioaerosols. In the present study 43 patients underwent conventional pulmonary function testing and lung scanning using small droplet (equal or less than 2 microns) polydisperse sup(99m)Tc-DTPA radioaerosol produced with the ''Settling Bag System'' Medi 400 (Sorin). The scans were analysed by two methods: 1) a semiquantitative method proposed by Taplin (SQT); 2) an original simpler semiquantitative method (SQM). Correlations of SQT and SQM with FEV1 and MEF75 resulted highly significant (p<0,001). SQM proved to be superior so SQT as far as reproducibility is concerned. The following conclusions can be drawn: 1) small particle sup(99m)Tc-DTPA aerosol can be easily and cheaply produced by a commercial device; 2) such a radioaerosol is well suited for the evaluation of small and large airways patency, as reflected by MEF75 and FEV1 respectively, with a diagnostic yield comparable to non-hydrosoluble radioaerosols; 3) the original semiquantitative description of the deposition pattern proposed and validated in this study is quite easy to implement and it yields a high correlation with pulmonary function tests; moreover such an approach does not require digital data processing; 4) the sensitivity of sup(99m)Tc-DTPA for the diagnosis of bronchial obstruction is very high and superior to routine pulmonary function tests; in addition the ventilation scan allows the topographical localization of the obstruction themselves

  20. Gd-DTPA Adsorption on Chitosan/Magnetite Nanocomposites

    OpenAIRE

    Pylypchuk, Ie. V.; Kołodyńska, D.; Kozioł, M.; Gorbyk, P. P.

    2016-01-01

    The synthesis of the chitosan/magnetite nanocomposites is presented. Composites were prepared by co-precipitation of iron(II) and iron(III) salts by aqueous ammonia in the 0.1 % chitosan solution. It was shown that magnetite synthesis in the chitosan medium does not affect the magnetite crystal structure. The thermal analysis data showed 4.6 % of mass concentration of chitosan in the hybrid chitosan/magnetite composite. In the concentration range of initial Gd-DTPA solution up to 0.4 mmol/L, ...

  1. MR imaging of the breast using Gd-DTPA

    International Nuclear Information System (INIS)

    One hundred selected patients underwent preoperative MR imaging of the breast Gd-DTPA. All carcinomas, fibroadenomas, and instances of mastitis enhanced significantly. Normal breast tissue, nonproliferative dysplasia, and scar tissue did not enhance. Borderline focal or generalized enhancement has been observed in cases of focal or generalized proliferative dysplasia. Compared to mammography, MR imaging yielded significant additional information in 20% of cases; the added information concerned mostly dense breasts and breasts with posttreatment changes. No additional information was obtained in fatty breasts, because of the high accuracy of mammography, and in breasts with proliferative dysplasia, because of their generalized enhancement

  2. Biliary enhanced MR imaging by Gd-DTPA

    Energy Technology Data Exchange (ETDEWEB)

    Ohkawa, Shinichi; Fujikura, Yuji; Kanai, Toshio (Hiratsuka City Hospital, Kanagawa (Japan)); Hiramatsu, Kyoichi

    1992-05-01

    Biliary enhanced MRI (BEMRI) by Gd-DTPA via PTCD and/or PTGBD tube for obstructive jaundice was performed in 8 patients. In all cases, biliary tract was clearly visualised as high signal intensity on T1 weighted images. On same images, primary lesion such as common bile duct cancer was also visualised as well as portal system. In addition, MR angiography (MRA) by 2D-time of flight method was performed. MRA with BEMRI shows portal encasement on the same image as biliary tract obstruction. This suggests MRA with BEMRI may replace the other modality for obstructive jaundice. (author).

  3. Improvement in DTPA efficacy for plutonium decorporation: biokinetic and dosimetric approaches

    Energy Technology Data Exchange (ETDEWEB)

    Serandour, A.L.; Grillon, G.; Taulelle, C.; Frechou, M.; Ben Salah, M.; Fritsch, P.; Le Gall, B.; Poncy, J.L. [CEA Bruyeres-le-Chatel (DSV/DRR), Lab. Radiotoxicologie, 91 (France); Gervelas, C.; Tsapis, N.; Fatal, E. [Paris-11 Univ., UMR CNRS 8612, Physico-chimie-Pharmacotechnie-Biopharmacie, 92 - Chatenay-Malabry (France); Phan, G.; Benech, H. [CEA Saclay (DSV/DRM), Service de Pharmacologie et d' Immunologie, 91 - Gif-sur-Yvette (France); Deverre, J.R. [Service Hospitalier Frederic Joliot (CEA/DSV/DRM), 91 - Gif-sur-Yvette (France)

    2006-07-01

    After accidental contamination, the main goal of decorporation is to reduce the dose delivered to the target tissues. In the case of plutonium, the main retention organs are the liver and the skeleton. Diethylenetriamine pentaacetic acid (DTPA ) is the only molecule efficient to de-corporate plutonium which can be administrated to humans. However, its efficiency is limited due to its fast urinary excretion and its distribution, mainly limited in blood and biological fluids. Our goal is to optimise the decorporation efficiency of DTPA by increasing its half-life in blood and allowing the concordance of distribution between the chelate and the actinide. We propose two novel formulations of DTPA according to the chemical form of radionuclides and the type of contamination. First we have shown that encapsulation of DTPA into liposomes could induce major modifications of the chelate pharmacokinetic parameters. For this study, DTPA was encapsulated into conventional and stealth liposomes (large and small vesicles) [1]. Their pharmacokinetics and ability to de-corporate Pu were evaluated in rats treated by a single injection of vectorized DTPA, two hours after contamination with colloidal or soluble Pu [2]. Results showed an increase of the plasma half-life of DTPA up to 12 h and an accumulation of DTPA in liver, spleen and bone. In addition, administration of liposomes containing DTPA induced a significant decrease of Pu retention in the liver (factor 2 compared to free DTPA ) and a large decrease of Pu deposition in the bone (factor 10 compared to free DTPA). These data were associated with an urinary excretion of 80% of injected Pu over 16 days. Thus, these formulations of DTPA encapsulated into liposomes were more efficient to de-corporate Pu than free DTPA. This novel de-corporating strategy could be efficiently used in contaminated patients to avoid long term retention of Pu in the body. Another approach consists in the development of a dry powder formulation of

  4. Improvement in DTPA efficacy for plutonium decorporation: biokinetic and dosimetric approaches

    International Nuclear Information System (INIS)

    After accidental contamination, the main goal of decorporation is to reduce the dose delivered to the target tissues. In the case of plutonium, the main retention organs are the liver and the skeleton. Diethylenetriamine pentaacetic acid (DTPA ) is the only molecule efficient to de-corporate plutonium which can be administrated to humans. However, its efficiency is limited due to its fast urinary excretion and its distribution, mainly limited in blood and biological fluids. Our goal is to optimise the decorporation efficiency of DTPA by increasing its half-life in blood and allowing the concordance of distribution between the chelate and the actinide. We propose two novel formulations of DTPA according to the chemical form of radionuclides and the type of contamination. First we have shown that encapsulation of DTPA into liposomes could induce major modifications of the chelate pharmacokinetic parameters. For this study, DTPA was encapsulated into conventional and stealth liposomes (large and small vesicles) [1]. Their pharmacokinetics and ability to de-corporate Pu were evaluated in rats treated by a single injection of vectorized DTPA, two hours after contamination with colloidal or soluble Pu [2]. Results showed an increase of the plasma half-life of DTPA up to 12 h and an accumulation of DTPA in liver, spleen and bone. In addition, administration of liposomes containing DTPA induced a significant decrease of Pu retention in the liver (factor 2 compared to free DTPA ) and a large decrease of Pu deposition in the bone (factor 10 compared to free DTPA). These data were associated with an urinary excretion of 80% of injected Pu over 16 days. Thus, these formulations of DTPA encapsulated into liposomes were more efficient to de-corporate Pu than free DTPA. This novel de-corporating strategy could be efficiently used in contaminated patients to avoid long term retention of Pu in the body. Another approach consists in the development of a dry powder formulation of

  5. A Facile Synthesis of Indole-based Conjugated Derivatives

    Institute of Scientific and Technical Information of China (English)

    LI, Qian-Qian; ZHU, Zhi-Chao; LI,Zhen; QIN, Jin-Gui

    2007-01-01

    A novel synthetic method was developed for the preparation of indole-based conjugated derivatives with satisfied yields. By applying this strategy, a series of new compounds were prepared conveniently. All the obtained new indole derivatives were characterized by spectroscopic analyse s, giving satisfactory data corresponding to their expected molecular structures.

  6. 3D-Gd-DTPA MR angiography of cerebral blood vessels: A comparison with conventional 3D-MR subtraction angiography

    International Nuclear Information System (INIS)

    Subtraction angiography based on 3D fast imaging techniques using rephasing and dephasing gradient pulses is widely used, resulting in angiograms which closely correlate with DSA. Similar angiograms can be obtained with 3D-gradient echo techniques before and after the administration of Gadolinium (Gd)-DTPA (0.1-0.2 mmol/kg) followed by image subtraction. As Gd-DTPA does not penetrate the blood-brain barrier, brain imaging is not altering by the contrast agent. Both techniques were compared in patients wihout vascular disease, venous sinus thrombosis, AV malformation and aneurysm. With both techniques the cerebro-vascular system was imaged favoring the venous system because of the more constant flow velocity and the lack of ECG synchronization. Gd-DTPA offers the advantage of the higher signal-to-noise ratio and is hampered by background superposition due to Gd-DTPA enhancement in non brain tissue. With both techniques vessels down to 1 mm can be imaged. (orig.)

  7. Gd-DTPA-enhanced MR in thoracic disc herniations

    Energy Technology Data Exchange (ETDEWEB)

    Parizel, P.M.; Rodesch, G.; Baleriaux, D.; Segebarth, C.; Zegers de Beyl, D.; Haens, J. d' ; Noterman, J.

    1989-03-01

    The Gd-DTPA-enhanced magnetic resonance findings in two patients with herniated thoracic intervertebral discs are reported. The first patient was a 56-year-old woman with a small subligamentous T6-7 disc herniation, slightly lateralized to the right. The second patient was a 51-year-old man with a central and right posterolateral disc herniation, including a large calcified fragment, at the T8-9 level. The nonenhanced MR examination revealed the presence of an extradural mass lesion in both patients, impinging upon the dural sac and compressing and displacing the spinal cord posteriorly. The lesion was slightly hypointense on both T1- and T2-weighted spin echo sequences. Following intravenous injection of Gd-DTPA in a dosage of 0.1 mmol/kg body weight, enhancement of the posterior longitudinal ligament was noted and triangular areas of contrast uptake were seen to occur in the epidural space above and below the herniated disc. At surgery, they were found to correspond to dilated and congested epidural veins.

  8. Respiratory clearance of 99mTc-DTPA and pulmonary involvement in sarcoidosis

    International Nuclear Information System (INIS)

    To investigate the relationships between the respiratory epithelial clearance of micronic aerosolized /sup 99m/Tc-DTPA (RC-DTPA) and pulmonary function, serum angiotensin-converting enzyme (SACE), and lymphocytic alveolitis in patients with sarcoidosis, RC-DTPA was measured in 49 nonsmokers with pulmonary sarcoidosis and 38 normal nonsmokers. Pulmonary involvement was evaluated on chest roentgenograms (type O = normal, type I = hilar adenopathies, type II = hilar adenopathies associated with parenchymal shadows, type III = parenchymal shadows without adenopathy) and by pulmonary function tests. Serum angiotensin-converting enzyme was determined, and a bronchoalveolar lavage was performed for alveolar lymphocyte differential counting (Ly%). RC-DTPA was increased (greater than or equal to 1.96%/min) in 12 of 31 patients with type II or III involvement but was normal in all 18 patients with type O or I involvement (p = 0.002). Patients with increased RC-DTPA had low FVC, TLC, FEV1, and resting Pao2 (p less than 0.05); resting and exercise AaPo2 were increased (p less than 0.05), but RC-DTPA correlated negatively with FEV1 (p less than 0.01), Pao2 at rest (p less than 0.005), and DLCO (p less than 0.05) and positively with resting and exercise AaPO2 (p less than 0.01). In patients with increased RC-DTPA (42 +/- 17%), Ly% did not differ from Ly% in patients with normal RC-DTPA (34 +/- 16%). SACE was increased in patients with increased RC-DTPA (56 +/- 26 U/ml versus 38 +/- 16 U/ml; p = 0.007) and correlated positively with RC-DTPA (p less than 0.001).(ABSTRACT TRUNCATED AT 250 WORDS)

  9. Dynamic 1H NMR Studies of Schiff Base Derivatives

    Science.gov (United States)

    Köylü, M. Z.; Ekinci, A.; Böyükata, M.; Temel, H.

    2016-01-01

    The spin-lattice relaxation time T 1 and the spin-spin relaxation time T 2 of two Schiff base derivatives, N,N'-ethylenebis(salicylidene)-1,2-diaminoethane (H2L1) and N,N'-ethylenebis (salicylidene)-1,3-diaminopropane (H2L2), in DMSO-d6 solvent were studied as a function of temperature in the range of 20-50°C using a Bruker Avance 400.132 MHz 1H NMR spectrometer. Based on the activation energy ( E a) and correlation time (τc), we believe that the Schiff base derivatives perform a molecular tumbling motion.

  10. Efficient in vitro labeling rabbit neural stem cell with paramagnetic Gd-DTPA and fluorescent substance

    International Nuclear Information System (INIS)

    Objectives: The aim of this study is to label rabbit neural stem cells (NSCs) by using standard contrast agents (Gd-DTPA) in combination with PKH26 and in vitro track them with MR imaging. Materials and methods: NSCs from prenatal brains of rabbits were cultured and propagated. Intracellular uptake of Gd-DTPA was achieved by using a non-liposomal lipid transfection reagent (Effectene) as the transfection agent. After labeling with Gd-DTPA, cells were incubated with cellular membrane fluorescent dye PKH26. The labeling effectiveness and the longevity of Gd-DTPA maintenance were measured on a 1.5 T MR scanner. The influence of labeling on the cellular biological behaviors was assessed by cellular viability, proliferation and differentiation assessment. Results: The labeling efficiency of Gd-DTPA was up to 90%. The signal intensity on T1-weighted imaging and T1 values of labeled cells were significantly higher than those of unlabeled cells (P 3. Cellular uptake of Gd-DTPA was maintained until 15 days after initially labeling. There was no significant difference in the cellular viability and proliferation between the labeled and unlabeled NSCs (P > 0.05). Normal glial and neuronal differentiation remained in labeled NSCs like unlabeled NSCs. Conclusion: Highly efficient labeling NSCs with Gd-DTPA could be achieved by using Effectene. This method of labeling NSCs allows for tracking cells with MR imaging, and without alterations of cellular biological behaviors.

  11. Exercise increases the lung clearance of inhaled Tc-99m DTPA

    International Nuclear Information System (INIS)

    It has been suggested that blood flow have little effect on the lung clearance of Tc-99m DTPA which depends on the alveolar distension. However an increase of pulmonary blood flow, induces a recruitment of new vascular areas which can enhance this clearance. To assess this hypothesis the pulmonary apical and basal clearances of a sumicronic aerosol of Tc-99m DTPA were studied at rest and at exercise in seven non smoking volunteers in upright posture. As a matter of fact exercising upright is known to increase primarily the apical blood flow. After inhalation the subjects were seated on a bicycle their back against a gamma camera which was linked to a computer. The lung radioactivity was registered successively during a resting and an exercising period. At rest there was a gradient of clearance from the apex to the base of the lung, the apical clearance being significantly higher. At exercise (50 Watts, 60 c. min /sup -1/, 7 min.) this regional gradient was enhanced by a large and significant increase of the apical clearances (0.0340 min /sup -1/ +-0.0063 SD versus 0.00183 min /sup -1/ +- 0.074 SD at rest, n = 7, p < 0.01). By contrast the changes of the basal clearances were slight and unsignificant (0.0146 min /sup -1/ +- 0.0062 SD versus 0.0140 min /sup -1/ +- 0.0082 SD). Since exercise induces little distension of the apical alveoli but drastically enhances the apical blood flow, the observed increase of the apical lung clearance could be primarily attributed to the recruitment of new capillaries induced by exercise in the apices. This led to an increase of the surface area permeability product

  12. Preparation of {sup 111}In-DTPA morpholino oligomer for low abdominal accumulation

    Energy Technology Data Exchange (ETDEWEB)

    Liu Guozheng, E-mail: guozheng.liu@umassmed.ed [Department of Radiology, University of Massachusetts Medical School, Worcester, MA 01655-0243 (United States); Dou Shuping; Rusckowski, Mary [Department of Radiology, University of Massachusetts Medical School, Worcester, MA 01655-0243 (United States); Greiner, Dale [Department of Medicine, University of Massachusetts Medical School, Worcester, MA 01655-0243 (United States); Hnatowich, Donald [Department of Radiology, University of Massachusetts Medical School, Worcester, MA 01655-0243 (United States)

    2010-09-15

    An ability to quantitate the beta cell mass by noninvasive nuclear imaging will be very useful in the prevention, diagnosis, and treatment of diabetes. However, to be successful, radioactivity from the pancreas must not be obscured by the background radioactivity in the abdomen. Pretargeting offers the promise of achieving high target organ to normal tissue ratios. In preparation for pancreas imaging studies by pretargeting using morpholino oligomers (MORF/cMORF), it was necessary to develop a simple and efficient method to radiolabel the cMORF effector. Because we have shown that labeling the cMORF with {sup 111}In via DTPA reduces excretion into the intestines compared to labeling with {sup 99m}Tc via MAG{sub 3}, the conjugation of DTPA to cMORF was investigated for {sup 111}In labeling. The amine-derivatized cMORF was conjugated with DTPA using 1-ethyl-3(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC) as an alternative to the conventional cyclic anhydride. The conjugation efficiency (represented by the number of DTPA groups attached per cMORF) was investigated by changing the EDC, DTPA, and cMORF molar ratios. Different open columns were considered for the purification of DTPA-cMORF. Before conjugation, each cMORF molecule was confirmed to have an amine by trinitrobenzene sulfonic acid (TNBS) assay using the {omega}-amino butyric acid as positive standard and the non-amine derivatized cMORF as negative standard. The average number of DTPA groups per cMORF was 0.15-0.20 following the conjugation over a cMORF/DTPA molar ratio of 0.5-5 and over a cMORF/EDC molar ratio of 20-60. The conjugation efficiency was lower than expected probably due to steric hindrance. A 1x50 cm P-4 column using ammonium acetate as eluting buffer provided an adequate separation of DTPA-cMORF from free DTPA. The {sup 111}In labeling efficiency by transchelation from acetate exceeded 95%, thus avoiding the need for postlabeling purification. Despite the lower than expected conjugation

  13. Gd-Complexes of New Arylpiperazinyl Conjugates of DTPA-Bis(amides): Synthesis, Characterization and Magnetic Relaxation Properties

    OpenAIRE

    Abdullah O. Ba-Salem; Nisar Ullah; M. Nasiruzzaman Shaikh; Mohamed Faiz; Zaheer Ul-Haq

    2015-01-01

    Two new DTPA-bis(amide) based ligands conjugated with the arylpiperazinyl moiety were synthesized and subsequently transformed into their corresponding Gd(III) complexes 1 and 2 of the type [Gd(L)H2O]·nH2O. The relaxivity (R1) of these complexes was measured, which turned out to be comparable with that of Omniscan®, a commercially available MRI contrast agent. The cytotoxicity studies of these complexes indicated that they are non-toxic, which reveals their potential and physiological suitabi...

  14. Use of paramagnetic chelated metal derivatives of polysaccharides and spin-labeled polysaccharides as contrast agents in magnetic resonance imaging

    Energy Technology Data Exchange (ETDEWEB)

    Bligh, S.W.; Harding, C.T.; Sadler, P.J.; Bulman, R.A.; Bydder, G.M.; Pennock, J.M.; Kelly, J.D.; Latham, I.A.; Marriott, J.A. (Department of Chemistry, Birkbeck College, London (England))

    1991-02-01

    Soluble and insoluble polysaccharides were derivatized with diethylenetriaminepentaacetic acid (DTPA) and/or spin-labeled with 2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO). Polysaccharides derivatized with DTPA were prepared via cyanogen bromide activation, coupling to a diamine linker, and to DTPA anhydride. Spin-labeled polysaccharides were also prepared via cyanogen bromide activation. The extent of derivatization for dextran (18 kDa) was about 120 glucose units per DTPA, and for cellulose and starch about 15-30 units per DTPA. For spin-labeled polysaccharides, the average loading ranged from 1 nitroxide per 16 glucose units for starch to 181 for dextran (82 kDa). These derivatized paramagnetic polysaccharides were shown to be more effective relaxants than the small paramagnetic molecules alone. Both soluble and insoluble polysaccharide-linker-DTPA-Gd(3) complexes were effectively cleared from the body (rats) after oral administration. After intravenous administration, the biodistribution of dextran-linker-DTPA-Gd(3) complexes differed significantly from that of GdDTPA. Reduction of the nitroxide by ascorbic acid was retarded in the polysaccharide derivatives, particularly in starch derivatized with both nitroxide and linker-DTPA-Cu(2). These agents showed contrast enhancement in the gastrointestinal tract of rabbits.

  15. Evaluation of inflammatory bowel disease with /sup 99mTc-DTPA

    Energy Technology Data Exchange (ETDEWEB)

    Kadir, S.; Strauss, H.W.

    1979-02-01

    Technetium-99m-labelled DTPA was found to localize in segments of bowel with inflammation due to ulcerative colitis, regional enteritis, and other forms of enterocolitis. The concentration of tracer was apparently related to the clinical activity of the disease process. Imaging with /sup 99m/Tc-DTPA may offer an appealing, noninvasive alternative for identifying and following up patients with inflammatory bowel disease.

  16. Study with radio aerosol of DTPA technetium-99 m in individuals with pulmonary disease by amiodarone

    International Nuclear Information System (INIS)

    In order to evaluate the role of the clearance of 99 m Technetium chelated to diethylenetriamine-penta-acetate (99 m Tc-DTPA) in amiodarone induced pulmonary disease, 40 individuals were studied in four groups. After spirometry, where a volume-time curve was registered, all individuals inhaled 740 MBq of 99 m Tc-DTPA diluted in 4 ml of saline, for five minutes. Pulmonary images were obtained in a computerized scintillation camera and 9 regions of interest were selected. (author)

  17. Oral Gd-DTPA as a negative gastrointestinal contrast agent in magnetic resonance cholangiopancreatography

    Institute of Scientific and Technical Information of China (English)

    陈燕萍; 张雪林; 昌仁民; 成官迅

    2003-01-01

    Objective: To evaluate the value of oral Gd-DTPA as a negative contrast agent during magnetic resonance cholangiopancreatography (MRCP) to eliminate the high signals of the gastrointestinal tract. Methods: To select the optimal concentration of oral Gd-DTPA for MRCP, a phantom study was performed followed by clinical trial in 15 cases undergoing MRCP before and after oral Gd-DTPA (in a total volume of 250 ml 1∶5 diluted Gd-DTPA, 1.488 g/L). MRCP images were acquired using two-dimensional single slice fast spin-echo (SSTSE) sequence and half-Fourier acquisition single slice fast spin-echo (HASTE) sequence. Results: The phantom study showed that the 1∶5 diluted oral Gd-DTPA was best in decreasing the signal intensity both in T2-weighted imaging (59.5%) and in HASTE sequence (82.45%). The high signal intensity of the stomach and intestinal fluid was completely suppressed in all the cases. The depictions of the common bile duct and pancreatic duct were markedly improved by using the oral contrast agent (P<0.05). Conclusion: Oral Gd-DTPA is effective and safe for eliminating the high signal of the gastrointestinal tract to improve the depiction of the biliary system by MRCP.

  18. The other membrane is the target of diethylenetriamine pentaacetic acid (DTPA) in cellular injury

    Energy Technology Data Exchange (ETDEWEB)

    Caceres, M.R.; Assis, M.L.B.; De Mattos, J.C.P.; Stunbo, A.C.; Carvalho, L.; Bernardo Filho, M.; Caldeira de Araujo, A. [Universidade do Estado, Rio de Janeiro, RJ (Brazil). Dept. de Biofisica e Biometria

    1997-12-31

    Full text. DTPA is the most commonly compound utilized for diagnostic imaging in nuclear medicine. After the conjugation to a radionuclide, such as {sup 99m} Tc, {sup 113m} In or {sup 111} In, DTPA allows satisfactory evaluation of esophageal transit, gastric emptying, glomerular filtration rate (GFR), blood-brain barrier (BBB), liver, and pulmonary system. However, our studies have shown a strong toxic effect in wild type bacterial cells (Escherichia coli AB 1157) caused by this drug, even when assayed in smaller concentration than administered to humans. DNA strand breaks analysis and cellular experiments in different mutant bacterial strain did not reveal any direct or indirect lesion in genetic material, respectively. Electron micrographs of treated E. coli demonstrated an irregular cell wall structure, which may result that some molecules present normally in extracellular environmental could exert their effect on intracellular metabolic processes. Since DTPA carry a chelator agent property, it suggest that DTPA take out Ca {sup +2} and/or other metallic ions present on membrane. Putting together, our results suggest that is essential the pH control of DTPA solution administered to patients. The role o DTPA on cellular membrane is still under investigation

  19. Preparation and characterization of new polyesters derived from schiff bases

    International Nuclear Information System (INIS)

    Summary: Three polyesters have been prepared by the reaction of terephthaloyl chloride on Schiff bases derived from 4- hydroxybenzyldehyde and meso- stilbenediamine, ethylenediamine or 4-nitrophenylenediamine.The polymers were obtained in good yield (85% theoritical) and were characterized by C,H,N elemental analyses, FT-IR, UV and visible spectroscopy and thermal analyses (TGA ,DTA). The results obtained were compared with that of Schiff base monomers .The viscosities measurements of the polyesters and their Schiff base monomers supported the polymers formation. (author)

  20. Design and functionalities of the MADOR® software suite for dose-reduction management after DTPA therapy.

    Science.gov (United States)

    Leprince, B; Fritsch, P; Bérard, P; Roméo, P-H

    2016-03-01

    A software suite on biokinetics of radionuclides and internal dosimetry intended for the occupational health practitioners of nuclear industry and for expert opinions has been developed under Borland C++ Builder™. These computing tools allow physicians to improve the dosimetric follow-up of workers in agreement with the French regulations and to manage new internal contaminations by radionuclides such as Pu and/or Am after diethylene triamine penta-acetic acid treatments. In this paper, the concept and functionalities of the first two computing tools of this MADOR(®) suite are described. The release 0.0 is the forensic application, which allows calculating the derived recording levels for intake by inhalation or ingestion of the main radioisotopes encountered in occupational environment. Indeed, these reference values of activity are convenient to interpret rapidly the bioassay measurements and make decisions as part of medical monitoring. The release 1.0 addresses the effect of DTPA treatments on Pu/Am biokinetics and the dose benefit. The forensic results of the MADOR(®) suite were validated by comparison with reference data.

  1. Design and functionalities of the MADORR software suite for dose-reduction management after DTPA therapy

    International Nuclear Information System (INIS)

    A software suite on biokinetics of radionuclides and internal dosimetry intended for the occupational health practitioners of nuclear industry and for expert opinions has been developed under Borland C++ BuilderTM. These computing tools allow physicians to improve the dosimetric follow-up of workers in agreement with the French regulations and to manage new internal contaminations by radionuclides such as Pu and/or Am after diethylene triamine penta-acetic acid treatments. In this paper, the concept and functionalities of the first two computing tools of this MADORR suite are described. The release 0.0 is the forensic application, which allows calculating the derived recording levels for intake by inhalation or ingestion of the main radioisotopes encountered in occupational environment. Indeed, these reference values of activity are convenient to interpret rapidly the bioassay measurements and make decisions as part of medical monitoring. The release 1.0 addresses the effect of DTPA treatments on Pu/Am biokinetics and the dose benefit. The forensic results of the MADORR suite were validated by comparison with reference data. (authors)

  2. Dynamic 99mTc-DTPA radioaerosol lung scanning

    International Nuclear Information System (INIS)

    51 patients (10 normal subjects and 41 pathological ones) underwent dynamic 99mTc-DTPA radioaerosol lung scanning to evaluate different technical aspects of alveolar-capillary permeability, as reflected by the rate constant (k) of the radiotracer exponential washout. A significant difference (p<0.01) was found between normal and pathological subjects but not among pathological groups as a damage of alveolar-capillary barrier is a non specific event common to different lung conditions. No significant correlation was found between total lung k and either spirometric tests (FEV1 and MEF75) or radioaerosol initial distribution, proving thus that the alveolar-capillary permeability is not related to derangements of ventilation. Inter and intraoperator reproducibilities were fairly satisfactory, showing correlation coefficients of 0.992 and 0.996, respectively. Sensitivity and specificity for the diagnosis of increased alveolar-capillary permeability were calculated using different criteria. The best results in the patients studied were achieved by using total rather than regional ks, leading to a specificity of 90% and a sensitivity of 86%. (orig.)

  3. DTPA: Bis benzimidazole as multi model imaging agent

    International Nuclear Information System (INIS)

    Full text: The DTPA bis benzimidazole analogue has been tested for radiopharmaceutical efficacy. The radiolabelling was found more then 98% after 8 hrs and blood kinetics was fast. The compound was also tested for optical imaging agent. The Eu3+ ion has an absorption band in the visible spectrum (578-582 nm) whose wavelength is very sensitive to even small changes in the coordination environment. Although the intensity of this 7F0 → 5D0 transition is low, the bands are relatively narrow, which allows distinguishing different coordination states of the metal. For Eu3+ complexes which have two differently hydrated forms in aqueous solution, one observes two absorption bands belonging to the two species. High-resolution UV-visible spectra were recorded in aqueous solutions which show a temperature invariant absorption with two distinct, temperature-dependent absorption bands. The intensity ratio of these two bands changes with temperature: the band at shorter wavelengths is decreasing very slightly, while that at longer wavelengths is increasing with the temperature. The ratio of the integrals of the two bands is related to the equilibrium constant, and its temperature dependence yields the reaction enthalpy and entropy

  4. Organic Field-effect Transistors Based on Tetrathiafulvalene Derivatives

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    1 Restults Tetrathiafulvalene (TTF) and its derivatives have been extensively investigated in the field of organic conductors and superconductors since 1973. Recently, their application in organic field-effect transistors (OFETs) has attracted considerable attention. So far, on the one hand, the fabrication techniques of the TTF-based FETs have been primarily limited to high vacuum evaporation, which is a relatively expensive process. On the other hand, low FET performances, such as the low on/off ratio...

  5. PRICING OF EXOTIC ENERGY DERIVATIVES BASED ON ARITHMETIC SPOT MODELS

    OpenAIRE

    FRED ESPEN BENTH; RODWELL KUFAKUNESU

    2009-01-01

    Based on a non-Gaussian Ornstein–Uhlenbeck model for energy spot, we derive prices for Asian and spread options using Fourier techniques. The option prices are expressed in terms of the Fourier transform of the payoff function and the characteristic functions of the driving noises, being independent increment processes. In many relevant situations, these functions are explicitly available, and fast Fourier transform can be used for efficient numerical valuation. The arithmetic nature of our m...

  6. Multivariable analysis of clinical influence factors on liver enhancement of Gd-EOB-DTPA-enhanced 3T MRI; Multivariable Analyse klinischer Einflussfaktoren auf die Signalintensitaet bei Gd-EOB-DTPA 3T-MRT der Leber

    Energy Technology Data Exchange (ETDEWEB)

    Verloh, N.; Haimerl, M.; Stroszczynski, C.; Fellner, C.; Wiggermann, P. [University Hospital Regensburg (Germany). Dept. of Radiology; Zeman, F. [University Hospital Regensburg (Germany). Center for Clinical Trials; Teufel, A. [University Hospital Regensburg (Germany). Dept. of Gastroenterology; Lang, S. [University Hospital Regensburg (Germany). Dept. of Surgery

    2015-01-15

    The purpose of this study was to identify clinical factors influencing Gd-EOB-DTPA liver uptake in patients with healthy liver parenchyma. A total of 124 patients underwent contrast-enhanced MRI with a hepatocyte-specific contrast agent at 3T. T1-weighted volume interpolated breath-hold examination (VIBE) sequences with fat suppression were acquired before and 20 minutes after contrast injection. The relative enhancement (RE) between plain and contrast-enhanced signal intensity was calculated. Simple and multiple linear regression analyses were performed to evaluate clinical factors influencing the relative enhancement. Patients were subdivided into three groups according to their relative liver enhancement (HRE, RE ≥ 100 %; MRE, 100 % > RE > 50 %; NRE, RE ≤ 50 %) and were analyzed according to the relevant risk factors. Simple regression analyses revealed patient age, transaminases (AST, ALT, GGT), liver, spleen and delta-liver volume (the difference between the volumetrically measured liver volume and the estimated liver volume based on body weight) as significant factors influencing relative enhancement. In the multiple analysis the transaminase AST, spleen and delta liver volume remained significant factors influencing relative enhancement. Delta liver volume showed a significant difference between all analyzed groups. Liver enhancement in the hepatobiliary phase depends on a variety of factors. Body weight-adapted administration of Gd-EOB-DTPA may lead to inadequate liver enhancement after 20 minutes especially when the actual liver volume differs from the expected volume.

  7. Comparison of 99mTc-DTPA GFR and 99mTc-DTPA Renal Clearance%肾动态体外摄取法GFR与99mTc-DTPA肾清除率的对比研究

    Institute of Scientific and Technical Information of China (English)

    齐慧敏; 辛岗; 张春丽; 李驚子; 邓丽荣; 王海燕

    2002-01-01

    Objective To evaluate the two methods in GFR measurements. Methods By using Receiver Operating Characteristic (ROC) curve, likelihood ratio and regression analysis we compared 99mTc- DTPA GFR with clearance of 99mTc-DTPA (CL 99mTc). Results The 99mTc - DTPA GFR mean area under ROC curve was 0.79 mean sensitivity was 62%, mean specificity was 83.8%, r = 0.78, regression formula was Y = 8.5 + 0.76X. Conclusion The accuracy of 99mTc - DTPA was not so good.

  8. Bile duct evaluation of potential living liver donors with Gd-EOB-DTPA enhanced MR cholangiography: Single-dose, double dose or half-dose contrast enhanced imaging

    International Nuclear Information System (INIS)

    Introduction: Detailed knowledge of the biliary anatomy is essential to avoid complications in living donor liver transplantation. The aim of this study was to determine the optimal dosage of Gd-EOB-DTPA for contrast-enhanced magnetic resonance cholangiography (ce-MRC) with reference to contrast-enhanced CT cholangiography (ce-CTC). Materials and methods: 30 potential living liver donors (PLLD) underwent both ce-CTC and ce-MRC. Ten candidates each received single, double or half-dose Gd-EOB-DTPA. Ce-MRC images with and without inversion recovery pulses (T1w ± IR) were acquired 20–30 min after intravenous contrast injection. Image data was quantitatively and qualitatively reviewed by two radiologists based on a on a 5-point scale. Data sets were compared using a Mann–Whitney-U-test or Wilcoxon-rank-sum-test. Kappa values were also calculated. Results: All image series provided sufficient diagnostic information both showing normal biliary anatomy and variant bile ducts. Ce-CTC showed statistically significant better results compared to all ce-MRC data sets. T1w MRC with single dose Gd-EOB-DTPA proved to be superior to half and double dose in subjective and objective evaluation without a statistically significant difference. Conclusions: Ce-MRC is at any dosage inferior to ce-CTC. As far as preoperative planning of bile duct surgery is focused on the central biliary anatomy, ce-MRC can replace harmful ce-CTC strategies, anyway. Best results were seen with single dose GD-EOB-DTPA on T1w MRC+IR

  9. Bile duct evaluation of potential living liver donors with Gd-EOB-DTPA enhanced MR cholangiography: Single-dose, double dose or half-dose contrast enhanced imaging

    Energy Technology Data Exchange (ETDEWEB)

    Kinner, Sonja, E-mail: Sonja.Kinner@uni-due.de [Department of Diagnostic and Interventional Radiology and Neuroradiology, University Hospital Essen (Germany); Steinweg, Verena [Department of Diagnostic and Interventional Radiology and Neuroradiology, University Hospital Essen (Germany); Maderwald, Stefan [Department of Diagnostic and Interventional Radiology and Neuroradiology, University Hospital Essen (Germany); Erwin L. Hahn Institute for Magnetic Resonance Imaging, Essen (Germany); Radtke, Arnold; Sotiropoulos, Georgios [Department of General Surgery, University Hospital Essen (Germany); Forsting, Michael; Schroeder, Tobias [Department of Diagnostic and Interventional Radiology and Neuroradiology, University Hospital Essen (Germany)

    2014-05-15

    Introduction: Detailed knowledge of the biliary anatomy is essential to avoid complications in living donor liver transplantation. The aim of this study was to determine the optimal dosage of Gd-EOB-DTPA for contrast-enhanced magnetic resonance cholangiography (ce-MRC) with reference to contrast-enhanced CT cholangiography (ce-CTC). Materials and methods: 30 potential living liver donors (PLLD) underwent both ce-CTC and ce-MRC. Ten candidates each received single, double or half-dose Gd-EOB-DTPA. Ce-MRC images with and without inversion recovery pulses (T1w ± IR) were acquired 20–30 min after intravenous contrast injection. Image data was quantitatively and qualitatively reviewed by two radiologists based on a on a 5-point scale. Data sets were compared using a Mann–Whitney-U-test or Wilcoxon-rank-sum-test. Kappa values were also calculated. Results: All image series provided sufficient diagnostic information both showing normal biliary anatomy and variant bile ducts. Ce-CTC showed statistically significant better results compared to all ce-MRC data sets. T1w MRC with single dose Gd-EOB-DTPA proved to be superior to half and double dose in subjective and objective evaluation without a statistically significant difference. Conclusions: Ce-MRC is at any dosage inferior to ce-CTC. As far as preoperative planning of bile duct surgery is focused on the central biliary anatomy, ce-MRC can replace harmful ce-CTC strategies, anyway. Best results were seen with single dose GD-EOB-DTPA on T1w MRC+IR.

  10. Nanoporous Carbide-Derived Carbon Material-Based Linear Actuators

    Directory of Open Access Journals (Sweden)

    Janno Torop

    2009-12-01

    Full Text Available Devices using electroactive polymer-supported carbon material can be exploited as alternatives to conventional electromechanical actuators in applications where electromechanical actuators have some serious deficiencies. One of the numerous examples is precise microactuators. In this paper, we show for first time the dilatometric effect in nanocomposite material actuators containing carbide-derived carbon (CDC and polytetrafluoroetylene polymer (PTFE. Transducers based on high surface area carbide-derived carbon electrode materials are suitable for short range displacement applications, because of the proportional actuation response to the charge inserted, and high Coulombic efficiency due to the EDL capacitance. The material is capable of developing stresses in the range of tens of N cm-2. The area of an actuator can be dozens of cm2, which means that forces above 100 N are achievable. The actuation mechanism is based on the interactions between the high-surface carbon and the ions of the electrolyte. Electrochemical evaluations of the four different actuators with linear (longitudinal action response are described. The actuator electrodes were made from two types of nanoporous TiC-derived carbons with surface area (SA of 1150 m2 g-1 and 1470 m2 g-1, respectively. Two kinds of electrolytes were used in actuators: 1.0 M tetraethylammonium tetrafluoroborate (TEABF4 solution in propylene carbonate and pure ionic liquid 1-ethyl-3-methylimidazolium trifluoromethanesulfonate (EMITf. It was found that CDC based actuators exhibit a linear movement of about 1% in the voltage range of 0.8 V to 3.0 V at DC. The actuators with EMITf electrolyte had about 70% larger movement compared to the specimen with TEABF4 electrolyte.

  11. Sidestream smoke inhalation decreases respiratory clearance of 99mTc-DTPA acutely

    International Nuclear Information System (INIS)

    The permeability of the alveolar-capillary barrier to an inhaled aerosol of technetium 99m labelled diethylenetriamine penta-acetate (99mTc-DTPA is used as an index of alveolar epithelial injury. Permeability is greatly increased in active smokers. The aim of this study was to determine the effect of sidestream smoke inhalation on permeability as this has not been described previously. Lung clearance of inhaled 99mTc-DTPA aerosol was measured in 20 normal non-smoking subjects before and after exposure to one hours sidestream smoke inhalation. Measured carbon monoxide (CO) levels rose to a maximum of 23.5 ±6.2 ppm from baseline values of 0.6±1.3 (p99mTc-DTPA clearance rose from baseline 69.1± 15.6 (mean ± to 77.4 ±17.8) after smoke exposure. No effect of 99mTc-DTPA scanning of sidestream smoke was demonstrated on lung function. It was concluded that low level sidestream smoke inhalation decreases 99mTc-DTPA clearance acutely in humans. The mechanism of this unexpected result is not established but may include differences in constituents between sidestream and mainstream smoke, alterations in pulmonary microvascular blood flow, or changes in surfactant due to an acute phase irritant response. 34 refs., 2 figs

  12. Study On The Preparation Of 90Y-DTPA-Rituximab For Non-Hodgkin Lymphoma Treatment

    International Nuclear Information System (INIS)

    Yttrium is one of the most useful radionuclides for radioimmunotherapeutic applications, especially labelling with monoclonal antibodies. Rituximab was bound to the DTPA chelating agent using Hnatowich methods. Cyclic anhydride DTPA (cDTPAa, 0.1 mg/ml) was dissolved in chloroform and was degassed under a stream of nitrogen for 30 minutes. Rituximab solution in 0.05 M bicarbonate buffer was immediately added and mixed for one minute at room temperature. The antibody Rituximab at different concentration (5 mg/ml and 10 mg/ml) was coupled with the cDTPAa, at molar ratios (cDTPAa : Rituximab) of 1:1, 3:1, 5:1, 10:1 and 20:1. The conjugation of DTPA-Rituximab mixture was labelled with Y-90, then using Sephadex G25 in order to determine coupling efficiency. Coupling efficiency at a 3:1 mole ratio was 70%. After purification, the conjugation DTPA-Rituximab was labeled with Y-90 in 0.5 M acetate buffer, pH 5, at room temperature. The labeling yield was about 99%. The radiochemical purity of 90Y-DTPA-Rituximab was more than 98 % which determined by ITLC in 0.1 M acetate at pH 6 as mobile phase. The radiopharmaceuticals have been test for sterility, apyrogenicity and biodistribution. This is a potential radiopharmaceutical for clinical application in therapeutic Non Hodgkin Lymphoma treatments. (author)

  13. Simplified structure of a new model to describe urinary excretion of plutonium after systemic, liver or pulmonary contamination of rats associated with Ca-DTPA treatments.

    Science.gov (United States)

    Fritsch, P; Sérandour, A L; Grémy, O; Phan, G; Tsapis, N; Abram, M C; Renault, D; Fattal, E; Benech, H; Deverre, J R; Poncy, J L

    2009-06-01

    This study validates, by targeted experiments, several modeling hypotheses for interpretation of urinary excretion of plutonium after Ca-DTPA treatments. Different formulations and doses of Ca-DTPA were administered to rats before or after systemic, liver or lung contamination with various chemical forms of plutonium. The biokinetics of plutonium was also characterized after i.v. injection of Pu-DTPA. Once formed, Pu-DTPA complexes are stable in most biological environments. Pu-DTPA present in circulating fluids is rapidly excreted in the urine, but 2-3% is retained, mainly in soft tissues, and is then excreted slowly in the urine after transfer to blood. Potentially, all intracellular monoatomic forms of plutonium could be decorporated after DTPA internalization involving slow urinary excretion of Pu-DTPA with half-lives varying from 2.5 to 6 days as a function of tissue retention. The ratio of fast to slow urinary excretion of Pu-DTPA depends on both plutonium contamination and Ca-DTPA treatment. Fast urinary excretion of Pu-DTPA corresponds to extracellular decorporation that occurs beyond a threshold of the free DTPA concentration in circulating fluids. Slow excretion corresponds mostly to intracellular decorporation and depends on the amount of intracellular DTPA. From these results, the structure of a simplified model is proposed for interpretation of data obtained with Ca-DTPA treatments after systemic, wound or pulmonary contamination by plutonium.

  14. Decorporation approach following rat lung contamination with a moderately soluble compound of plutonium using local and systemic Ca-DTPA combined chelation.

    Science.gov (United States)

    Grémy, Olivier; Tsapis, Nicolas; Bruel, Sylvie; Renault, Daniel; Van der Meeren, Anne

    2012-09-01

    Decorporation efficacy of prompt pulmonary delivery of DTPA dry powder was assessed following lung contamination with plutonium nitrate and compared to an intravenous injection of DTPA solution and a combined administration of both DTPA compounds. In addition, efficacy of a delayed treatment was assessed. In case of either early or late administration, insufflated DTPA was more efficient than intravenously injected DTPA in reducing the plutonium lung burden due to its high local concentration. Prompt treatment with DTPA powder was also more effective in limiting extrapulmonary deposits by removing the early transportable fraction of plutonium from lungs prior its absorption into blood. Translocation of DTPA from lungs to blood may also contribute to the decrease in extrapulmonary retention, as shown by reduced liver deposit after delayed pulmonary administration of DTPA. Efficacy of DTPA dry powder was further increased by the combined intravenous administration of DTPA solution for reducing extrapulmonary deposits of plutonium and promoting its urinary excretion. According to our results, the most effective treatment protocol for plutonium decorporation was the early pulmonary delivery of DTPA powder supplemented by an intravenous injection of DTPA solution. Following inhalation of plutonium as nitrate chemical form, this combined chelation therapy should provide a more effective method of treatment than conventional intravenous injection alone. At later stages following lung contamination, pulmonary administration of DTPA should also be considered as the treatment of choice for decreasing the lung burden.

  15. Lung function declines in patients with pulmonary sarcoidosis and increased respiratory epithelial permeability to 99mTc-DTPA

    International Nuclear Information System (INIS)

    Respiratory epithelial clearance of 99mTc-DTPA (RC-Tc-DTPA) and pulmonary function tests (PFT) were determined at intervals of 6 or 12 months in 37 untreated, nonsmoking patients with sarcoidosis over a period of 6 to 36 months. PFT included the measurements of total lung capacity (TLC), vital capacity (VC), FEV1, and diffusing capacity for carbon monoxide. No difference was found between the respiratory clearance of 113mIn-DTPA (2.25 +/- 1.00%/min) and RC-Tc-DTPA (2.29 +/- 1.11%/min) in eight patients with pulmonary sarcoidosis. Pulmonary function decreased 15% or more in at least 2 function tests during 11 follow-up periods, but it remained stable during 47 follow-up periods. In patients whose lung function deteriorated, RC-Tc-DTPA increased to 3.51 +/- 1.55%/min; in contrast, in patients whose lung function remained stable, regardless of the initial values, RC-Tc-DTPA was normal (1.00 +/- 0.50%/min; p less than 0.001). In eight patients who were treated with corticosteroids, RC-Tc-DTPA decreased from 3.48 +/- 1.31%/min to 1.56 +/- 0.64%/min (p less than 0.001), and PFT improved. We conclude that in nonsmokers with pulmonary sarcoidosis, increased RC-Tc-DTPA is not related to dissociation of 99mTc from DTPA, RC-Tc-DTPA is increased when pulmonary function decreases, and, when increased, RC-Tc-DTPA decreases with corticosteroid therapy

  16. Single-molecular diodes based on opioid derivatives.

    Science.gov (United States)

    Siqueira, M R S; Corrêa, S M; Gester, R M; Del Nero, J; Neto, A M J C

    2015-12-01

    We propose an efficient single-molecule rectifier based on a derivative of opioid. Electron transport properties are investigated within the non-equilibrium Green's function formalism combined with density functional theory. The analysis of the current-voltage characteristics indicates obvious diode-like behavior. While heroin presents rectification coefficient R>1, indicating preferential electronic current from electron-donating to electron-withdrawing, 3 and 6-acetylmorphine and morphine exhibit contrary behavior, Rdiodes. In particular, the rectification rations for heroin diodes show microampere electron current with a maximum of rectification (R=9.1) at very low bias voltage of ∼0.6 V and (R=14.3)∼1.8 V with resistance varying between 0.4 and 1.5 M Ω. Once most of the current single-molecule diodes usually rectifies in nanoampere, are not stable over 1.0 V and present electrical resistance around 10 M. Molecular devices based on opioid derivatives are promising in molecular electronics.

  17. Single-molecular diodes based on opioid derivatives.

    Science.gov (United States)

    Siqueira, M R S; Corrêa, S M; Gester, R M; Del Nero, J; Neto, A M J C

    2015-12-01

    We propose an efficient single-molecule rectifier based on a derivative of opioid. Electron transport properties are investigated within the non-equilibrium Green's function formalism combined with density functional theory. The analysis of the current-voltage characteristics indicates obvious diode-like behavior. While heroin presents rectification coefficient R>1, indicating preferential electronic current from electron-donating to electron-withdrawing, 3 and 6-acetylmorphine and morphine exhibit contrary behavior, Rresonant-tunneling diodes. In particular, the rectification rations for heroin diodes show microampere electron current with a maximum of rectification (R=9.1) at very low bias voltage of ∼0.6 V and (R=14.3)∼1.8 V with resistance varying between 0.4 and 1.5 M Ω. Once most of the current single-molecule diodes usually rectifies in nanoampere, are not stable over 1.0 V and present electrical resistance around 10 M. Molecular devices based on opioid derivatives are promising in molecular electronics. PMID:26613894

  18. Dye-sensitized solar cells based on bisindolylmaleimide derivatives

    Institute of Scientific and Technical Information of China (English)

    Qiong ZHANG; Zhijun NING; Hongcui PEI; Wenjun WU

    2009-01-01

    Three organic dyes based on bisindolylmaleimide derivatives (11, 12 and 13) were synthesized and investigated as sensitizers for the application in nanocrystalline TiO2 solar cells. The indole group,maleimide group and carboxylic group functioned as electron donor, acceptor and anchoring group, respec-tively. Solar-to-electrical energy conversion efficiencies under simulated amplitude-modulated 1.5 irradiation based on 12 and of 1.87% and 1.50% for 13 and 11,respectively. The open circuit voltage Voc was demon-strated to be enhanced by the introduction of dodecyl or benzyl moieties on the indole groups. The nonplanar structure of bisindolylmaleimide was proven to be effective in aggregation resistance. This work suggests that organic sensitizers with maleimide as electron acceptor are promising candidates as organic sensiti-zers in dye-sensitized solar cells.

  19. Early detection of amphotericin B induced nephrotoxicity by 99mTc-DTPA: a useful test

    International Nuclear Information System (INIS)

    Amphotericin B (AMB) with a broad spectrum of antifungal activity is used for the treatment of life-threatening mycoses, especially in immunocompromised patients. Since measurements of the blood level of AMB and of creatinine do not provide early warning of AMB induced renal toxicity, we studied the effects of AMB on biodistribution of a glomerular agent, 99mTc-DTPA. In Swiss mice, the toxicity of AMB was studied at single intravenous doses of 0.5-3.5 mg/kg body weight. Dose dependent effects consisted of decreased blood clearance and urinary excretion. Recovery of function was shown in dose ranges corresponding to high level clinical schedules. Serum creatinine changes lagged behind the99m Tc-DTPA alterations. This suggests that AMB toxicity might be monitored by blood/urinary clearance of 99mTc-DTPA

  20. Animal experiment of 111In-DTPA-D-Phe1-octreotide

    International Nuclear Information System (INIS)

    The animal experiments of a novel somatostatin receptor-positive tumors imaging agent, 111In-DTPA-D-Phe1-octreotide, has been described. Biological properties were evaluated by dynamic and static γ images of somatostatin receptor-positive tumors in nude mice bearing pancreatic carcinoma. The radiocomponent of 111In-DTPA, which came from 111In-DTPA-D-Phe1-octreotide decomposition, showed 50% of radioactivity in blood and 40% in urine at 3h postinjection. Rapid blood and urine clearance, and high T/B ratio (7.92 up to 24 h postinjection) were observed. The images of somatostatin receptor-positive tumors could be obtained during 0.5-24 h after administration but the best one would be at 24 h

  1. Synthesis and Characterization of 2-Decyl-DTPA and Its Gd(Ⅲ) Chelate

    Institute of Scientific and Technical Information of China (English)

    FENG Zhi-ming; LI Feng; CHEN Rong; ZHU Xiao-juan; LI Xiao-ru

    2004-01-01

    The present paper covers the synthesis and the characterization of ligand 2-decyl-3, 6, 9-tris(carboxymethyl)-3,6,9-triazaundecan-1,11-dioic acid, H5L, and its Gd(Ⅲ) chelate. The protonation constants for H5L(lgKHi=10.90, 8.50, 4.55, 2.92, 2.20) and the stability constant for GdL2- (lgKGdL2-=22.80) were determined by means of potentiometric titration. They are similar to the corresponding values of DTPA and Gd-DTPA, respectively. The results obtained show that the basicity of the ligand and the stability constant of its Gd(Ⅲ) chelate are not obviously altered after the introduction of a linear chain decyl group into the terminal acetic acid residue of DTPA. The Gd(Ⅲ) chelate may be a potential contrast agent with liver-specificity for magnetic resonance imaging(MRI).

  2. Easy and Efficient 111Indium Labeling of Long-Term Stored DTPA Conjugated Protein

    DEFF Research Database (Denmark)

    Nalla, Amarnadh; Buch, Inge; Hesse, Birger

    2011-01-01

    -diethylene-triamine-pentaacetic acid (cDTAP) was conjugated to a polyclonal immunoglobulin-G (IgG) in borate buffer, pH 8.2 at +4?C for 4 hours. Then the DTPA-conjugate was dialyzed against 50 mmol/l sodium citrate buffer saline, pH 6.0 and stored at -80° C in aliquots of 1 mg/0.5 ml. The DTPA-conjugate was labeled with 111In......-chloride in citrate buffer, pH 6. The labelling reaction was incubated at room temperature (RT) for 30 min and at +4?C for 90 min. Determination of labelling efficiency was performed using ITLC and an instant chromatography scanner equipped with a NaI crystal. The labelling efficiency of the DTPA...

  3. Easy and efficient (111)Indium labeling of long-term stored DTPA conjugated protein

    DEFF Research Database (Denmark)

    Nalla, Amarnadh; Buch, Inge; Hesse, Birger

    2011-01-01

    -diethylene-triamine-pentaacetic acid (cDTAP) was conjugated to a polyclonal immunoglobulin-G (IgG) in borate buffer, pH 8.2 at +4oC for 4 hours. Then the DTPA-conjugate was dialyzed against 50 mmol/l sodium citrate buffer saline, pH 6.0 and stored at -80o C in aliquots of 1 mg/0.5 ml. The DTPA-conjugate was labeled with 111In......-chloride in citrate buffer, pH 6. The labelling reaction was incubated at room temperature (RT) for 30 min and at +4oC for 90 min. Determi- nation of labelling efficiency was performed using ITLC and an instant chromatography scanner equipped with a NaI crystal. The labelling efficiency of the DTPA...

  4. Mass effect and Pu removal from rats with Ca- or Zn-DTPA

    International Nuclear Information System (INIS)

    Eight percent of an intramuscular injection of 27.6 nCi of 237Pu(NO3)4 was retained by rats at the injection site, after 22 days. More than 30% is usually retained following injection of more massive quantities of 239Pu. Treatment with Ca- or Zn-DTPA showed the former to be more effective when treatments were initiated 1 hr after Pu administration. When treatments were begun 7 days after the Pu injection, 416 nmol Ca-DTPA/kg was more effective in removing Pu than 28.7 nmol/kg (human dose level) of Zn- or Ca-DTPA. Due to its high specific activity and ease of detection, 237Pu permits facile experimentation in small animals at Pu mass levels comparable to those encountered in most human exposures

  5. Reverse lung clearance of Tc-99m DTPA induced by postural changes

    International Nuclear Information System (INIS)

    The lung clearance of Tc 99m DTPA is generally attributed to the degree of distension of the interepithelial junctions and thus of the alveoli. To assess this hypothesis a submicronic aerosol of Tc 99m DTPA (0.5 μ mass median diameter) was inhaled by eight non smoking volunteers placed in left lateral decubitus (LLD) where the alveoli of the left lung are compressed and those of the right lung expanded. The lung radioactivity was followed with a gamma camera 15 minutes in LLD, then 15 minutes in right lateral decubitus (RLD) to reverse the relative distension of the alveoli. Regional clearances were computed on each lung divided in three horizontal slices. These results suggest that alveolar distension has a major role in the DTPA clearance which is relatively independent of the initial deposition of the aerosol

  6. Studies on the biological behaviors of taxol derivatives

    Energy Technology Data Exchange (ETDEWEB)

    Awh, Ok Doo; Yoo, Dae Wung [College of Medicine, Yonsei Univ., Wonju (Korea, Republic of); Im, Sang Moo [College of Medicine, Korea Cancer Center, Seoul (Korea, Republic of)

    1997-07-01

    This study was designed to prospect the {sup 111}In-labelled paclitaxel as tumor imaging agent. In order to provide a taxol molecule with a functional -group which is able to chelate In-111, taxol-DTPA conjugate and 2'-hemisuccinyltaxol were synthesized by esterification of taxol at C-2' on C-13 carbon with DTPA anhydride and succinic anhydride, respectively. Synthesis yield of the taxol derivatives was 34% for taxol-DTPA and 80% for 2'-hemisuccinyltaxol. Cytotoxicity of the taxol derivatives were measured by MTT method toward cell lines HT29, B16, P388, and CT26. The cytotoxic activities of the taxol derivatives were maintained, although less active than taxol. Radiolabelling of the taxol derivatives were proceeded directly with {sup 111}InCl{sub 3} or indirectly with {sup 111}In-citrate(ligand-exchange method). The ligand-exchange method was not suitable because some precipitates appeared during the reaction. On the contrary, by direct radiolabelling method, we were able to obtain taxol-DTPA-{sup 111}In in 100% radiochemical yield. However, 2'-hemisuccinyltaxol was not labelled by both methods. Yield and radiochemical purity of the radiolabelled compound were determined by HPLC, paper chromatography and instant thin layer chromatography. Taxol-DTPA -{sup 111}In was characterized to be hydrophilic by lipophilicity test, and nearly non-adhesive to HT29, B16, P388, and CT26 by cell binding affinity test. Binding affinity of the taxol-DTPA-{sup 111}In complex to serum proteins was also examined by protein precipitation with 30% trichloroacetic acid. The results showed that 30% of the taxol-DTPA-{sup 111}In complex binds with serum proteins.

  7. In vivo evaluation of carborane gadolinium-DTPA complex as an MR imaging boron carrier

    International Nuclear Information System (INIS)

    The evaluation of the Gd-carborane complex 2 as an MR imaging and boron carrier agent was carried out in vivo using tumor-bearing Donryu rats, MRI, ICP-AES, and α-autoradiography. The MR imaging revealed that the carborane Gd-DTPA 2 was metabolized slower in the body than Gd-DTPA 1. The results of the ICP-AES method indicated that compound 2 was incorporated into normal tissues and metabolized quickly, whereas it was not accumulated into tumor or brain tissue. The α-autoradiography showed that a high level of boron was obtained in the internal organs and in the necrosis of tumor tissue. (author)

  8. Role of Gd-DTPA in the differentiation of posttreatment changes recurrent musculoskeletal sarcomas

    International Nuclear Information System (INIS)

    This paper evaluates Gd-DTPA enhancement prospectively in 35 patients with treated musculoskeletal sarcomas (13 osteosarcomas, eight malignant fibrous histiocytomas, four synovial sarcomas). Thirteen patients with osteosarcoma received intraarterial chemotherapy 3 months to 2 years before surgery. Others received pre-and/or postoperative intravenous chemotherapy. Some also received external irradiation. Multiplanar images of the tumors were obtained on a 1.5-T Signa imager (General Electric) with a spin-echo technique. T1- and T2-weighted images were compared with enhanced T1-weighted images (intravenous Gd-DTPA, 0.1 mmol-kg)

  9. Salicylic acid failed to increase the efficacy of Ca-DTPA in the decorporation of plutonium and americium

    International Nuclear Information System (INIS)

    Male and female C57BL/Do mice were each given a single i.p. injection of 237+239Pu + 241Am as the citrate complex at 45 days of age. Twice weekly i.p. injecctions of either 500 μmol/kg Ca-DTPA or 500 μmol/kg Ca-DTPA, mixed just before injection with 2000 μmol/kg salicylic acid (SA), were begun 3 days after nuclide administration and continued for 5 weeks. Control mice were injected each time with isotonic saline. Nuclide retention was determined by in vivo counting using NaI(T1) spectrometry. At the end of treatment, total-body retention of Pu or Am in the mice given Ca-DTPA was significantly lower (P < 0.001) than in the control animals. Mice treated with Ca-DTPA + SA were statistically indistinguishable from mice treated with Ca-DTPA

  10. Salicylic acid failed to increase the efficacy of Ca-DTPA in the decorporation of plutonium and americium

    International Nuclear Information System (INIS)

    Male and female C57BL/Do mice were each given a single ip injection of /sup 237 + 239/Pu + 241Am as the citrate complex at 45 days of age. Twice-weekly ip injections of either 500 μmole/kg Ca-DTPA or 500 μmole/kg Ca-DTPA mixed just before injection with 2000 μmole/kg salicylic acid (SA) were begun 3 days after nuclide administration and continued for 5 weeks. Control mice were injected each time with isotonic saline. Nuclide retention was determined by in vivo γ-ray counting using a NaI(Tl) crystal spectrometer. At the end of treatment, total-body retention of Pu or Am in the mice given Ca-DTPA was significantly lower (P < 0.001) than that in the control animals. Mice treated with Ca-DTPA plus SA were statistically indistinguishable from mice treated with Ca-DTPA alone

  11. Gd-Complexes of New Arylpiperazinyl Conjugates of DTPA-Bis(amides: Synthesis, Characterization and Magnetic Relaxation Properties

    Directory of Open Access Journals (Sweden)

    Abdullah O. Ba-Salem

    2015-04-01

    Full Text Available Two new DTPA-bis(amide based ligands conjugated with the arylpiperazinyl moiety were synthesized and subsequently transformed into their corresponding Gd(III complexes 1 and 2 of the type [Gd(LH2O]·nH2O. The relaxivity (R1 of these complexes was measured, which turned out to be comparable with that of Omniscan®, a commercially available MRI contrast agent. The cytotoxicity studies of these complexes indicated that they are non-toxic, which reveals their potential and physiological suitability as MRI contrast agents. All the synthesized ligands and complexes were characterized with the aid of analytical and spectroscopic methods, including elemental analysis, 1H-NMR, FT-IR, XPS and fast atom bombardment (FAB mass spectrometry.

  12. Aerosolized gadolinium-DTPA for demonstration of pulmonary ventilation in MR imaging of the lung

    International Nuclear Information System (INIS)

    Purpose: Magnetic resonance assessment of lung ventilation with aerosolized Gd-DTPA. Methods: Eleven experimental procedures were carried out in a domestic pig model. The intubated pigs were aerosolized for 30 minutes with an aqueous formulation of Gd-DTPA. The contrast agent aerosol was generated by a small particle aerosol generator. Imaging was performed on a 1.5 T MR imager using a T1-weighted turbo spin echo sequence with respiratory gating (TR 141 ms, TE 8.5 ms, 6 averages, slice thickness 10 mm). Pulmonary signal intensities before and after ventilation were measured in peripheral portions of both lungs. Results: Immediately after ventilation with aerosolized Gd-DTPA, the signal intensity in both lungs increased significantly in all animals with values up to 237% above baseline (mean 139%±48%), but within some cases considerable regional intra- and interindividual intensity differences. Distinctive parenchymal enhancement was readily visualized in all eleven cases with good spatial resolution. Conclusion: The presented data indicate that Gd-DTPA in aerosolized form can be used to demonstrate pulmonary ventilation in large animals with lung volumes comparable to man. Further experimental trials are necessary to improve reproducibility and to define the scope of this method for depicting lung disease. (orig.)

  13. Vesicoureteral Reflux Detected with 99mTc-DTPA Renal Scintigraphy during Evaluation of Renal Function

    Directory of Open Access Journals (Sweden)

    Nevena Manevska

    2015-12-01

    Full Text Available BACKGROUND: Radionuclide techniques, as direct radionuclide cystography and 99mTc-DMSA scintigraphy, have been used in evaluation of vesicoureteral reflux (VUR and reflux nephropathy (RN in children. Dynamic 99mTc-DTPA scintigraphy is reserved for evaluation of differential renal function and obstruction in children, where hydronephrosis is detected by ultrasonography (US pre- or postnatally. CASE REPORT: Six year old boy was prenatally diagnosed with bilateral hydronephrosis. Postnatal, severe bilateral VUR was detected by voiding urethrocytography. US and 99mTc-DTPA scintigraphy performed in the first month of life showed small left kidney that participated with 2% in the global renal function. Bilateral cutaneous ureterostomy has been performed in order to obtain good renal drainage and promote optimal renal growth. Twelve months later, classic antireflux procedure was done. Control 99mTc-DTPA scintigraphy, 5 ys after antireflux surgery, revealed persisting radioactivity during the diuretic phase, in the left kidney that indicated antireflux procedure failure with VUR reappearance. CONCLUSION: 99mTc-DTPA scintigraphy is the first method of choice for long-term monitoring of individual kidney function in children with VUR and other congenital urinary tract anomalies. Additionally, it can be used as indirect radionuclide cystography when rising of radioactivity in the kidney region, during the diuretic phase can indicate presence of VUR.

  14. Vesicoureteral Reflux Detected with 99mTc-DTPA Renal Scintigraphy during Evaluation of Renal Function

    Science.gov (United States)

    Manevska, Nevena; Stojanoski, Sinisa; Majstorov, Venjamin; Pop-Gjorcheva, Daniela; Zdraveska, Nikolina; Kuzmanovska, Dafina

    2016-01-01

    BACKGROUND: Radionuclide techniques, as direct radionuclide cystography and 99mTc-DMSA scintigraphy, have been used in evaluation of vesicoureteral reflux (VUR) and reflux nephropathy (RN) in children. Dynamic 99mTc-DTPA scintigraphy is reserved for evaluation of differential renal function and obstruction in children, where hydronephrosis is detected by ultrasonography (US) pre- or postnatally. CASE REPORT: Six year old boy was prenatally diagnosed with bilateral hydronephrosis. Postnatal, severe bilateral VUR was detected by voiding urethrocytography. US and 99mTc-DTPA scintigraphy performed in the first month of life showed small left kidney that participated with 2% in the global renal function. Bilateral cutaneous ureterostomy has been performed in order to obtain good renal drainage and promote optimal renal growth. Twelve months later, classic antireflux procedure was done. Control 99mTc-DTPA scintigraphy, 5 ys after antireflux surgery, revealed persisting radioactivity during the diuretic phase, in the left kidney that indicated antireflux procedure failure with VUR reappearance. CONCLUSION: 99mTc-DTPA scintigraphy is the first method of choice for long-term monitoring of individual kidney function in children with VUR and other congenital urinary tract anomalies. Additionally, it can be used as indirect radionuclide cystography when rising of radioactivity in the kidney region, during the diuretic phase can indicate presence of VUR. PMID:27275347

  15. Evaluation of In-111 DTPA-paclitaxel scintigraphy to predict response on murine tumors to paclitaxel

    International Nuclear Information System (INIS)

    Our goal was to determine whether scintigraphy with 111In-DTPA-paclitaxel could predict the response to chemotherapy with paclitaxel. Ovarian carcinoma (OCA 1), mammary carcinoma (MCA-4), fibrosarcoma (FSA) and squamous cell carcinoma (SCC VII) were inoculated into the thighs of female C3Hf/Kam mice. Mice bearing 8 mm tumors were treated with paclitaxel (40 mg/kg). The growth delay, which was defined as the time in days for tumors in the treated groups to grow from 8 to 12 mm in diameter minus the time in days for tumors in the untreated control group to reach the same size, was measured to determine the effect of paclitaxel on the tumors. Sequential scintigraphy in mice bearing 10 to 14 mm tumors was conducted at 5, 30, 60, 120, 240 min and 24 hrs postinjection of 111In-DTPA-paclitaxel (3.7 MBq) or 111In-DTPA as a control tracer. The tumor uptakes (% injection dose/pixel) were determined. The growth delay of OCA 1, MCA-4, FSA and SCC VII tumors was 13.6, 4.0, -0.02 and -0.28 days, respectively. In other words, OCA 1 and MCA-4 were paclitaxel-sensitive tumors, whereas FSA and SCC VII were paclitaxel-resistant tumors. The tumor uptakes at 24 hrs postinjection of In-111 DTPA paclitaxel of OCA 1, MCA-4, FSA and SCC VII were 1.0 x 10-3, 1.6 x 10-3, 2.2 x 10-3 and 9.0 x 10-3% injection dose/pixel, respectively. There was no correlation between the response to chemotherapy with paclitaxel and the tumor uptakes of 111In-DTPA-paclitaxel. Scintigraphy with 111In-DTPA-paclitaxel could not predict the response to paclitaxel chemotherapy. Although there was significant accumulation of the paclitaxel in the tumor cells, additional mechanisms must be operative for the agent to be effective against the neoplasm. 111In-DTPA-paclitaxel activity is apparently different from that of paclitaxel with Cremophor. (author)

  16. Assessment of alveolar epithelial permeability in Behcet's disease with 99mTc-DTPA aerosol scintigraphy

    International Nuclear Information System (INIS)

    Behcet's disease (BD) is a multisystem disorder characterized by vasculitis, and consists of a triad of recurrent ulcers of the oral and genital mucosa with relapsing uveitis. The prevalance of pulmonary involvement varies in the range of 1-10% in various studies and its complications are severe and life threatening. In this study, we investigated the changes of pulmonary epithelial permeability of patients with BD using technetium-99m diethylene triamine penta-acetic acid (99mTc-DTPA) aerosol scintigraphy, so as to begin the therapy regimen as soon as possible. Twenty-one nonsmoking patients with BD (8 women, 13 men; mean age 38.67±8.86 years) and 15 healthy volunteer nonsmoking controls (8 women, 7 men; mean age 50.87±12.45 years) underwent 99mTc-DTPA aerosol inhalation scintigraphy and pulmonary function tests (PFTs). Subjects inhaled 1480 MBq of 99mTc-DTPA for 4 min in the supine position. Scintigraphic data were recorded dynamically (1 frame/min) in the posterior projection on a 64 x 64 matrix for a 30-min period using a double-headed gamma camera (Infinia, GE, Tirat Hacarmel, Israel) equipped with a low-energy all-purpose parallel hole collimator. Half time of 99mTc-DTPA clearance (T1/2) was calculated by placing a mono-exponential fit on the curves. Penetration index (PI) was also calculated by dividing the peripheral total counts by the sum of the peripheral and central total counts on the first minute image, in order to quantify the distribution of the inhaled aerosol. The clearance half time of 99mTc-DTPA radioaerosols in the BD patients (24.81±6.22 min) was faster than in the normal control group (46.53±22.41 min) (P=0.004). There was also a significant difference between PI of the patients with BD (0.15±0.03) and that of the controls (0.21±0.06) (P=0.002). No correlation was found between the mean T1/2 values of 99mTc-DTPA clearance or the spirometric measurements in the BD patients. Penetration indices were not correlated with PET in the BD

  17. Factors in myocardial "perfusion" imaging with ultrafast MRI and Gd-DTPA administration.

    Science.gov (United States)

    Burstein, D; Taratuta, E; Manning, W J

    1991-08-01

    Ultrafast magnetic resonance imaging (MRI) and first pass observation of an interstitial contrast agent are currently being used to study myocardial perfusion. Image intensity, however, is a function of several parameters, including the delivery of the contrast agent to the interstitium (coronary flow rate and diffusion into the interstitium) and the relaxation properties of the tissue (contrast agent concentration, proton exchange rates, and relative intra- and extracellular volume fractions). In this study, image intensity during gadopentetate dimeglumine (Gd-DTPA) administration with T1-weighted ultrafast MR imaging was assessed in an isolated heart preparation. With increasing Gd-DTPA concentration, the steady-state myocardial image intensity increased but the time to reach steady state remained unchanged, resulting in an increased slope of image intensity change. A range of physiologic perfusion pressures (and resulting coronary flow rates) had insignificant effects on kinetics of Gd-DTPA wash-in or steady-state image intensity, suggesting that diffusion of Gd-DTPA into the interstitium is the rate limiting step in image intensity change with this preparation. Following global ischemia and reperfusion, transmural differences in the slope of image intensity change were apparent. However, the altered steady-state image intensity (due to postischemic edema) makes interpretation of this finding difficult. The studies described here demonstrate that although Gd-DTPA administration combined with ultrafast imaging may be a sensitive indicator of perfusion abnormalities, factors other than perfusion will affect image intensity. Extensive studies will be required before image intensity with this protocol is fully understood. PMID:1775055

  18. Novel and facile methods for the synthesis of DTPA-mono-amide. A new completely revised strategy in radiopharmaceutical chemistry

    International Nuclear Information System (INIS)

    DTPA is a very strong metal chelator widely utilized in radiopharmaceutical chemistry for conjugation of chemicals which do not have enough potency for direct metalo-labeling and also to manage toxic radioactive materials such as plutonium, americium, and curium. It is difficult to conjugate DTPA to an amine group in a singular direction and such reactions usually also coincidently produce a mixture of DTPA-bis-amides and DTPA-mono-amide resulting in considerable insufficiencies/difficulties in synthesis and especially yield/separation procedures. In this paper, novel methods for the exclusive synthesis of DTPA-mono-amide have been established which extensively reduce the difficulties otherwise encountered and increase the reaction's yield considering the green chemistry approaches. This is expected to be of interest to radiopharmaceutical researchers interested in the DTPA (Radio)-metallic-conjugate. Overall, this paper provides a framework to achieve a higher degree of propriety from DTPA as a chelator to conjugate to the chemical compounds. (author)

  19. INSURANCE CONTRACTS BASED ON INDICES, A STEP TOWARDS WEATHER DERIVATIVES

    Directory of Open Access Journals (Sweden)

    Boton#537; Horia-Mircea

    2012-07-01

    Full Text Available The aim of this paper is to analyze the methodology of structuring an index based insurance contract. The weather has always been the biggest risk factor in agriculture, affecting all aspects of the economic sectors. In the developed countries of the world, there is a significant number of rain-dependent farmers. To gain insight into potential growth of weather markets in developed countries, we are going to analyze the case of India. In 2003, Basix microfinance company in Hyderabad and ICICI Lombard insurance company in Mumbai have launched a pilot weather insurance, which had as basic index phenomena for Mahahbubnagar district of Andhra Pradesh. Further detail can be found in the full-paper. In the paper we will enumerate the appropriate steps in the determining the necessity for the use of index based insurances. The appropriate stages are making a comparison between index based insurances and traditional insurances, and optimizing the use of weather indices in insurance contracts. In order for the contracts to be successful , the recommended steps are : developing a product based in an index, and after the we need to plan and implement the contract. In planning the implementation it is needed that we: identify the risks and areas where they manifest, then identify the best distribution channels; after this we can develop a prototype of the contract. The next step would the testing of the negotiability of the contract, then the contract is opened to be finalized. After this the index based insurance contract can be introduced to the market, but the program needs monitoring in order to insure its successfulness. In order for the market and contract to grow the necessary step are: having access to the necessary meteorological data, determine the optimal way of integrating the contract in the existing economical context, technical feasibility, property rights and the legal framework. In the end, the aim is to familiarize the literacy field and

  20. A study on the short-term metabolism of curium and its removal with DTPA in the rat

    International Nuclear Information System (INIS)

    The contents of 242Cm in the plasma, liver, skeleton and kidneys were determined from 22.5 minutes to 16 days after the intravenous injection of 242Cm citrate to the rat. The 242Cm injected disappeared from the plasma rapidly and deposited mainly in the liver and skeleton. By analyzing the relationship between the disappearance from the plasma and the accumulation in the organs of injected curium, the initial distribution space, or the actual site of transfer compartment used for dose calculation in the ICRP Publ.30, of curium was identified as the extracellular fluid (ECF). The calculation showed that the difference between the radiation dose to bone surface or red bone marrow from short-lived curium isotopes evaluated under the current ICRP assumption, on one hand, and the same radiation dose, evaluated under the assumption that the actual site of transfer compartment is ECF, on the other, is not negligible. The effect of administration of CaDTPA or ZnDTPA on the retention of 242Cm in organs was studied by changing the time interval between the 242Cm injection and DTPA administration and the amount of DTPA administered. The results obtained showed that 1) DTPA treatment of a person who has incorporated curium into the body should be started as early as possible, 2) the first single prompt DTPA should be administered as Ca salt, and the following multiple delayed DTPA, as the less toxic Zn salt, and 3) the dose of first CaDTPA should be as large as possible within the range not producing its side effect. The study on the mixture of 242Cm and 238Pu showed that these two radioelements are metabolized and removed with DTPA independently. (author)

  1. A novel polymeric herbicide based on phenoxyacetic acid derivatives

    Directory of Open Access Journals (Sweden)

    Wimol Klaichim

    2009-01-01

    Full Text Available A novel polymeric herbicide based on phenoxyacetic acid derivatives was prepared by the reaction of epoxidised liquid natural rubber (ELNR with 2,4-dichlorophenoxyacetic acid (2,4-D or 2-methyl-4-chlorophenoxyacetic acid(MCPA. The liquid natural rubber (LNR was firstly obtained from the degradation of natural rubber latex with tert-butyl hydroperoxide and cobalt acetylacetonate at 65oC for 72 hrs. The epoxidised liquid natural rubber was prepared from thereaction of LNR with formic acid and hydrogen peroxide at 50oC for 6 hrs. The reaction of epoxidised liquid natural rubber with 2,4-D or MCPA using triethylamine as a catalyst in toluene was performed at 70, 80, and 90oC for 6, 9, 12, 18, and 24hrs. The polymeric herbicides obtained were characterized and the grafting percentage of 2,4-D or MCPA onto liquid natural rubber were also determined by FT-IR and 1H-NMR spectroscopy. It was found that the grafting percentage increased with increasing amount of reactants, temperature, and reaction time. The release of 2,4-D and MCPA from polymeric herbicides was investigated in pH 6, 7, and 8 buffers at room temperature. The results show that the slowest release of 2,4-D and MCPA was found to be constant at pH 7 for 14 and 10 days, respectively.

  2. Multifunctional switches based on bis-imidazole derivative

    Indian Academy of Sciences (India)

    Abdullah M A Asiri; Gameel A Baghaffar; Khadija O Badahdah; Abdullah G M Al-Sehemi; Salman A Khan; Abeer A Bukhari

    2009-11-01

    multifunctional bis-imidazole derived from piperonal was prepared and found to have photo, thermo, solvato and peiezochromism with colour changes from pale green to deep blue. The multifunctionality colour changes and stability of the coloured species make the derivative candidates for various applications such as optical data storage. The photochromic properties and performance were found to be affected remarkably upon changing the solvent.

  3. Theoretical Design of Blue-light-emitting Material Based on 1,2,3-Benzotriazole-based Derivative

    Institute of Scientific and Technical Information of China (English)

    HU Bo; YAO Chan; HUANG Xu-Ri

    2011-01-01

    Theoretically,1,2,3-benzotriazole(BT)-based derivative is designed by the struc-tural tuning in 2,1,3-benzothiadiazole(BTD)-based derivative and presents potential for applications in organic light-emitting diodes(OLEDs).Calculations show that the emission spectrum of BT-based derivative is located at the blue scope,so it can act as a blue-light-emitting material.Importantly,the oscillator strength of emission spectrum is significantly enhanced by replacing BTD with BT,implying it possess large fluorescent intensity.Additionally,BT-based derivative exhibits improved hole transportation with respect to the BTD-based derivative.

  4. Evaluation of nuclear imaging for detecting posttransplant fluid collection. [Comparison of /sup 99/Tc-DTPA imaging and ultrasonography for detection of fluid accumulation or lymphoceles

    Energy Technology Data Exchange (ETDEWEB)

    Burt, R.W. (Indiana Univ. Medical Center, Indianapolis); Reddy, R.K.

    1979-07-01

    A series of 26 renal allograft recipients studied by both B-mode gray scale ultrasonography and /sup 99m/Tc-DTPA renal nuclear imaging was reviewed. All conclusions were based on written reports generated at the original examination, and B-mode gray scale ultrasonography was considered the definitive examination for fluid collection detection. Sensitivity of nuclear imaging for the detection of fluid was 73%; however, it becomes 85% when fluid collections in close proximity to the bladder are excluded. Renal allograft nuclear imaging is a useful examination for primary detection of lymphoceles and also for demonstrating abnormalities in renal dynamics and drainage.

  5. Radiation protect during the ventilation scintigraphy of Tc99m DTPA radioaerosol in pediatric application

    Energy Technology Data Exchange (ETDEWEB)

    Chen, Yu-Wen; Dai, Zen-Kong; Huang, Ying-Fong; Jong, Shiang-Bing [Chou-Hon Memorial Hospital, Kaohsiung Medical Univ., Kaohsiung, Taiwan (China)

    2000-05-01

    Lung ventilation-perfusion scintigraphy is of great value for the management of patients with both primary lung disease and heart disease, by proving patho- physiological information of importance for the diagnosis, follow-up and functional evaluation of the patients. Krypton 81m radioactive gas is preferable for pediatric application due to its short half-life. However, the rubidium-krypton 81m generator is not popular in hospital of our country. Tc99m DTPA radioaerosol ventilation scintigraphy has its unique convenient for clinical application. But, the most disadvantage of clinical application of Tc99m DTPA radioaerosol is contamination of environment when the poor-cooperative patient can't breathe by mouth. For this reason, we design the certain procedure to reduce the radioaerosol contamination. During May to Aug., 1999, we collect 36 pediatric patients (male to female ratio 2:1, age from 6 months to 20 years old) with clinical history of lung or heart disease, including congenital heart disease, asthma and so on. Before the cases receive 10 to 15 mCi Tc99m DTPA radioaerosol ventilation scan, all of them were trained with breath training. And during the ventilation scintigraphy, the special mouth mask is designed to prevent the radioaerosol leakage into atmosphere. Then Geiger-Muller survey meter was arranged to detect the environmental contamination of radioaerosol in the mask, one and two metes away from the mask every 10 minutes during ventilation scintigraphy procedure and 1 hour after finishing image. Two nuclear medicine physicians evaluated imaging quality of ventilation scintigraphy. Results: Among thirty-six pediatric patients with prior breath training, thirty-two cases are successful to proceed the Tc99m DTPA ventilation scintigraphy. The other four cases that were under three-year-old fail to receive ventilation scintigraphy. There is limited detectable radioactivity in the mouth mask at early 10 minute by Geiger-Muller counter. No significant

  6. MR imaging of tumor angiogenesis using sterically stabilized Gd-DTPA liposomes targeted to CD105

    International Nuclear Information System (INIS)

    Aim: To depict tumor angiogenesis via the expression of CD105 in tumor-bearing rats using Gd-DTPA liposomes targeted to CD105 (CD105-Gd-SLs) on MR imaging. Materials and methods: Three Gd-DTPA liposomal nanoparticles were prepared in our trial: liposomes entrapping Gd-DTPA (Gd-SLs), Gd-SLs conjugated to immunoglobulins (IgG-Gd-SLs) and CD105-Gd-SLs. Forty glioma-bearing rats were randomized into four groups: (a) Gd-DTPA; (b) Gd-SLs; (c) IgG-Gd-SLs; (d) CD105-Gd-SLs. Axial T1WI MRI images were collected at baseline and repeated at 5, 30, 60 and 120 min post-intravenous injection of Gd-DTPA or liposome. Enhancement features and contrast-to-noise ratio of each group were analyzed. After imaging, tumors were resected for immunohistochemistry and immunofluorescence staining to assess vascularity and angiogenesis. Results: The four groups showed different enhancement features. The enhancement area was restricted for group CD105-Gd-SLs, while diffused for the other three. The degree of enhancement over time varied: group Gd-DTPA showed an early contrast enhancement at instant after injection with a peak at 30 min and a decline to baseline values at 60 min. In group CD105-Gd-SLs, the signal intensity (SI) continuously increased over 120 min. In groups IgG-Gd-SLs and Gd-SLs the SI peaked at 60 min, followed by a minor decrease for IgG-Gd-SLs and a rapid decrease for Gd-SLs almost to baseline. Immunohistochemistry and immunofluorescence showed that the enhancement in the CD105-Gd-SLs group resulted mainly from new microvessels. While in the other three groups, mature microvessels and new microvasculature resulted in the enhancement of the tumor. Conclusion: CD105-Gd-SLs can be used to detect early tumor angiogenesis on MR images. This might provide a means to non-invasively reveal a malignant phenotype of extracerebral F98 tumor and evaluate its progression.

  7. Fluorescent sensor based on a novel conjugated polyfluorene derivative

    Science.gov (United States)

    Gao, Weiqiang; Yan, Mei; Ge, Shenguang; Liu, Xiaoxia; Yu, Jinghua

    2012-09-01

    A novel water-soluble polyfluorene derivative, poly[(9,9-bis(3'-((N,N-dimethylamino)N-ethylammonium)propyl)-2,7-fluorene)-alt-2,7-(9,9-p-divinylbenzene)]dibromide (P-2) was synthesized by the palladium-catalyzed Suzuki coupling reaction and it's quaternized ammonium polyelectrolyte derivatives was obtained through a postpolymerization treatment on the terminal amino groups. The electrochemical and optical properties of the copolymers was fully investigated. The results showed that the new polyfluorene derivative had high electronic conductivity and strong fluorescence, therefore it had good potential to be used in chemical and biological sensors, as shown in optical sensing of bovine albumin (BSA) in this study.

  8. Personal Identification Based on Vectorcardiogram Derived from Limb Leads Electrocardiogram

    Directory of Open Access Journals (Sweden)

    Jongshill Lee

    2012-01-01

    Full Text Available We propose a new method for personal identification using the derived vectorcardiogram (dVCG, which is derived from the limb leads electrocardiogram (ECG. The dVCG was calculated from the standard limb leads ECG using the precalculated inverse transform matrix. Twenty-one features were extracted from the dVCG, and some or all of these 21 features were used in support vector machine (SVM learning and in tests. The classification accuracy was 99.53%, which is similar to the previous dVCG analysis using the standard 12-lead ECG. Our experimental results show that it is possible to identify a person by features extracted from a dVCG derived from limb leads only. Hence, only three electrodes have to be attached to the person to be identified, which can reduce the effort required to connect electrodes and calculate the dVCG.

  9. Glomerular filtration rate estimated from the uptake phase of 99mTc-DTPA renography in chronic renal failure

    DEFF Research Database (Denmark)

    Petersen, L J; Petersen, J R; Talleruphuus, U;

    1999-01-01

    The purpose of the study was to compare the estimation of glomerular filtration rate (GFR) from 99mTc-DTPA renography with that estimated from the renal clearance of 51Cr-EDTA, creatinine and urea.......The purpose of the study was to compare the estimation of glomerular filtration rate (GFR) from 99mTc-DTPA renography with that estimated from the renal clearance of 51Cr-EDTA, creatinine and urea....

  10. Personal Identification Based on Vectorcardiogram Derived from Limb Leads Electrocardiogram

    OpenAIRE

    Jongshill Lee; Youngjoon Chee; Inyoung Kim

    2012-01-01

    We propose a new method for personal identification using the derived vectorcardiogram (dVCG), which is derived from the limb leads electrocardiogram (ECG). The dVCG was calculated from the standard limb leads ECG using the precalculated inverse transform matrix. Twenty-one features were extracted from the dVCG, and some or all of these 21 features were used in support vector machine (SVM) learning and in tests. The classification accuracy was 99.53%, which is similar to the...

  11. Accuracy and confidence of Gd-EOB-DTPA enhanced MRI and diffusion-weighted imaging alone and in combination for the diagnosis of liver metastases

    International Nuclear Information System (INIS)

    Purpose: To evaluate the accuracy and confidence in diagnosing liver metastases using combined gadolinium-EOB-DTPA (Gd-EOB-DTPA) enhanced magnetic resonance imaging (MRI)/diffusion-weighted imaging (DWI) in comparison to Gd-EOB-DTPA enhanced MRI and DWI alone. Materials and methods: Forty-three patients (age, 58 ± 13 years) with 89 liver lesions (28 benign, 61 malignant) underwent liver MRI for suspected liver metastases. Three image sets (DWI, Gd-EOB-DTPA and combined Gd-EOB-DTPA/DWI) in combination with unenhanced T1- and T2-weighted images were reviewed by three readers. Detection rates of focal liver lesions were assessed and diagnostic accuracy was evaluated by calculating the areas under the receiver-operating-characteristics curve (AUC). Confidence in diagnosis was evaluated on a 3-point scale. Histopathology and imaging follow-up served as the standard of reference. Results: Detection of liver lesions and confidence in final diagnosis for all readers were significantly higher for the combined Gd-EOB-DTPA/DWI dataset than for DWI. The combination of DWI and Gd-EOB-DTPA rendered a significantly higher confidence in final diagnosis (2.44 vs. 2.50) than Gd-EOB-DTPA alone for one reader. For two readers, accuracy in diagnosis of liver metastases was significantly higher for Gd-EOB-DTPA/DWI (AUCs of 0.84 and 0.83) than for DWI datasets (AUCs of 0.73 and 0.72). Adding DWI to Gd-EOB-DTPA did not significantly increase diagnostic accuracy as compared to Gd-EOB-DTPA imaging alone. Conclusion: Addition of DWI sequences to Gd-EOB-DTPA enhanced MRI did not significantly increase diagnostic accuracy as compared to Gd-EOB-DTPA enhanced MRI alone in the diagnosis of liver metastases. However, the increase in diagnostic confidence might justify acquisition of DWI sequences in a dedicated MRI protocol

  12. Accuracy and confidence of Gd-EOB-DTPA enhanced MRI and diffusion-weighted imaging alone and in combination for the diagnosis of liver metastases

    Energy Technology Data Exchange (ETDEWEB)

    Donati, Olivio F.; Fischer, Michael A.; Chuck, Natalie; Hunziker, Roger [Institute of Diagnostic and Interventional Radiology, University Hospital Zurich, Zurich (Switzerland); Weishaupt, Dominik [Institute of Radiology, Triemli Hospital, Zurich (Switzerland); Reiner, Caecilia S., E-mail: caecilia.reiner@usz.ch [Institute of Diagnostic and Interventional Radiology, University Hospital Zurich, Zurich (Switzerland)

    2013-05-15

    Purpose: To evaluate the accuracy and confidence in diagnosing liver metastases using combined gadolinium-EOB-DTPA (Gd-EOB-DTPA) enhanced magnetic resonance imaging (MRI)/diffusion-weighted imaging (DWI) in comparison to Gd-EOB-DTPA enhanced MRI and DWI alone. Materials and methods: Forty-three patients (age, 58 ± 13 years) with 89 liver lesions (28 benign, 61 malignant) underwent liver MRI for suspected liver metastases. Three image sets (DWI, Gd-EOB-DTPA and combined Gd-EOB-DTPA/DWI) in combination with unenhanced T1- and T2-weighted images were reviewed by three readers. Detection rates of focal liver lesions were assessed and diagnostic accuracy was evaluated by calculating the areas under the receiver-operating-characteristics curve (AUC). Confidence in diagnosis was evaluated on a 3-point scale. Histopathology and imaging follow-up served as the standard of reference. Results: Detection of liver lesions and confidence in final diagnosis for all readers were significantly higher for the combined Gd-EOB-DTPA/DWI dataset than for DWI. The combination of DWI and Gd-EOB-DTPA rendered a significantly higher confidence in final diagnosis (2.44 vs. 2.50) than Gd-EOB-DTPA alone for one reader. For two readers, accuracy in diagnosis of liver metastases was significantly higher for Gd-EOB-DTPA/DWI (AUCs of 0.84 and 0.83) than for DWI datasets (AUCs of 0.73 and 0.72). Adding DWI to Gd-EOB-DTPA did not significantly increase diagnostic accuracy as compared to Gd-EOB-DTPA imaging alone. Conclusion: Addition of DWI sequences to Gd-EOB-DTPA enhanced MRI did not significantly increase diagnostic accuracy as compared to Gd-EOB-DTPA enhanced MRI alone in the diagnosis of liver metastases. However, the increase in diagnostic confidence might justify acquisition of DWI sequences in a dedicated MRI protocol.

  13. Clearance of 99mTc-DTPA and experimentally increased alveolar surfactant content

    International Nuclear Information System (INIS)

    The authors measured clearance of 99mTc-labeled diethylenetriamine pentaacetic acid (99mTc-DTPA) in rabbits with experimentally increased alveolar surfactant content. In one group of animals, surfactant production was increased by treatment with ambroxol, and another group of animals was treated with tracheal instillation of natural surfactant. A group of untreated control animals and animals treated with instillation of saline were also studied. Clearance was measured during standard conditions of mechanical ventilation and during ventilation with large tidal volumes. In ambroxol- and surfactant-treated groups, clearance rate was reduced compared with untreated control animals. In contrast, clearance rate increased after saline instillation. The differences were observed at both modes of ventilation. The findings indicate that the pulmonary surfactant system is a rate-limiting factor for the clearance of 99mTc-DTPA and that the volume dependence of clearance is not explained by stretching of the alveolar wall only. 28 refs., 2 figs., 1 tab

  14. Gadolinium-DTPA: value in MR imaging of extraspinal musculoskeletal infections

    Energy Technology Data Exchange (ETDEWEB)

    Haddad, M.C. [Dept. of Radiology, Riyadh Armed Forces Hospital, Riyadh (Saudi Arabia); Sharif, H.S. [Dept. of Radiology, Riyadh Armed Forces Hospital, Riyadh (Saudi Arabia); Aabed, M.Y. [Dept. of Radiology, Riyadh Armed Forces Hospital, Riyadh (Saudi Arabia); Al Shahed, M.S. [Dept. of Radiology, Riyadh Armed Forces Hospital, Riyadh (Saudi Arabia); Sammak, B.M. [Dept. of Radiology, Riyadh Armed Forces Hospital, Riyadh (Saudi Arabia); Clark, D.C. [Dept. of Radiology, Riyadh Armed Forces Hospital, Riyadh (Saudi Arabia)

    1993-12-01

    To determine if paramagnetic contrast agents can improve the detection, delineation, and characterization of extraspinal musculoskeletal infections (MSI) on magnetic resonance (MR) imaging, 42 patients with clinical suspicion of MSI underwent MR imaging before and after intravenous administration of gadolinium-DTPA. The lesions consisted of 27 proven infections and 15 noninfective conditions. Specificity and accuracy in identifying infective lesions averaged 80% and 84%, respectively, on precontrast studies and 80% and 89% on the enhanced examinations, with no statistically significant difference. Rim enhancement around abscess loculi was the only pathognomonic sign of infection seen in ten patients with chronic osteomyelitis and pyogenic or tuberculous infections. In 17 patients with acute osteomyelitis, brucellosis, or mycetoma, detection and delineation of the lesions were best on precontrast studies, while postcontrast examinations resulted in underestimation of the extent of abnormalities in all cases. We conclude that intravenous gadolinium-DTPA has limited usefulness in the MR evaluation of extraspinal MSI. (orig.)

  15. Calcium and zinc DTPA administration for internal contamination with plutonium-238 and americium-241.

    Science.gov (United States)

    Kazzi, Ziad N; Heyl, Alexander; Ruprecht, Johann

    2012-08-01

    The accidental or intentional release of plutonium or americium can cause acute and long term adverse health effects if they enter the human body by ingestion, inhalation, or injection. These effects can be prevented by rapid removal of these radionuclides by chelators such as calcium or zinc diethylenetriaminepentaacetate (calcium or zinc DTPA). These compounds have been shown to be efficacious in enhancing the elimination of members of the actinide family particularly plutonium and americium when administered intravenously or by nebulizer. The efficacy and adverse effects profile depend on several factors that include the route of internalization of the actinide, the type, and route time of administration of the chelator, and whether the calcium or zinc salt of DTPA is used. Current and future research efforts should be directed at overcoming limitations associated with the use of these complex drugs by using innovative methods that can enhance their structural and therapeutic properties.

  16. Gadolinium-DTPA-enhanced magnetic resonance imaging of musculoskeletal infectious processes

    Energy Technology Data Exchange (ETDEWEB)

    Hopkins, K.L. [Dept. of Diagnostic Radiology, Stanford Univ. Medical Center, CA (United States); Li, K.C.P. [Dept. of Diagnostic Radiology, Stanford Univ. Medical Center, CA (United States); Bergman, G. [Dept. of Diagnostic Radiology, Stanford Univ. Medical Center, CA (United States)

    1995-07-01

    The purpose of this study was to assess whether gadolinium-enhanced magnetic resonance imaging (MRI) provides diagnostic information beyond that given by nonenhanced imaging in the evaluation of musculoskeletal infectious processes and whether it can be used for differentiating infectious from noninfectious inflammatory lesions. Magnetic resonance images performed with and without intravenous gadolinium-DTPA in 34 cases in which musculoskeletal infection had been clinically suspected were reviewed. Infectious lesions-including osteomyelitis, pyarthrosis, abscess, and cellulitis-were confirmed in a total of 22 cases: in 15 by biopsy or drainage and in 7 by clinical course. Our results show that gadolinium-DTPA-enhanced MRI is a highly sensitive technique in diagnosing musculoskeletal infectious lesions. It is especially useful in distinguishing abscesses from surrounding cellulitis/myositis. Lack of contrast enhancement rules out infection with a high degree of certainty. However, contrast enhancement cannot be used to reliably distinguish infectious from noninfectious inflammatory conditions. (orig.)

  17. Clearance of technetium-99m-DTPA and HRCT findings in the evaluation of patients with Idiopathic Pulmonary Fibrosis

    Directory of Open Access Journals (Sweden)

    Karkavitsas Nikolaos

    2006-02-01

    Full Text Available Abstract Background Clearance of inhaled technetium-labeled diethylenetriamine pentaacetate (99mTc-DTPA is a marker of epithelial damage and an index of lung epithelial permeability. The aim of this study was to investigate the role of 99mTc-DTPA scan in patients with Idiopathic Pulmonary Fibrosis (IPF. Our hypothesis is that the rate of pulmonary 99mTc-DTPA clearance could be associated with extent of High Resolution Computed Tomography (HRCT abnormalities, cell differential of bronchoalveolar lavage fluid (BALF and pulmonary function tests (PFTs in patients with IPF. Methods We studied prospectively 18 patients (14 male, 4 female of median age 67yr (range 55–81 with histologically proven IPF. HRCT scoring included the mean values of extent of disease. Mean values of these percentages represented the Total Interstitial Disease Score (TID. DTPA clearance was analyzed according to a dynamic study using a Venticis II radioaerosol delivery system. Results The mean (SD TID score was 36 ± 12%, 3 patients had mild, 11 moderate and 4 severe TID. Abnormal DTPA clearance half-time (t1/2 Conclusion Our data suggest that 99mTc-DTPA lung scan is not well associated with HRCT abnormalities, PFTs, and BALF cellularity in patients with IPF. Further studies in large scale of patients are needed to define the role of this technique in pulmonary fibrosis.

  18. The preparation and identification of 99mTc-DTPA-HSA instant kit for blood-pool imaging

    International Nuclear Information System (INIS)

    The procedures for the preparation of animal experiment and preliminary clinical application of 99mTc-DTPA-HSA instant kit are described. The data showed that the radiochemical purity of this preparation was 97%, and also was stable more than 3 hours in room temperature. A comparison of 99mTc-DePA-HSA and 99mTc-HSA was carried in rats. The disappearance of radioactivity from the blood solwer for 99mTc-DTPA-HSA (T1/2 269.7 min, 88.8% of injected dose remaining in the blood at 1 hour) that for 99mTc-HSA (T1/2 = 139.6 min, 78.5% of injected dose remaining in the blood at 1 hour). In rabbits the blood pool images of 99mTc-DTPA-HSA was much more clear than with 99mTc-HSA. Identification of radioactive metabolites excreted in rabbit's urine 1 hour after injection of 99mTc-DTPA-HSA or indicated that no decomposition of 99mTc-DTPA-HSA was found, while in of 99mTc-HSA degradation was obvious. The liver blood pool imaging and the gated cardiac blood pool imaging were performed using 99mTc-DTPA-HSA demonstrating satisfactory results in 10 cases and also no toxic effects were observed

  19. Evaluation of lung epithelial permeability in the volatile substance abuse using Tc-99m DTPA aerosol scintigraphy

    International Nuclear Information System (INIS)

    Chronic inhalant use is associated with significant toxic effects, including neurological, renal, hepatic, and pulmonary damage. However, there is a paucity of reports regarding respiratory complications in inhalant abusers. The aim of this study was to evaluate pulmonary epithelial permeability in the volatile substance abuse (VSA) using technetium-99m-labeled diethylenetriamine pentaacetic acid (Tc-99m DTPA) aerosol scintigraphy. This study included 18 patients with volatile substance abuse and 18 volunteer controls. All of patients and controls were smokers. Tc-99m DTPA aerosol scintigraphy was performed in all cases. Time-activity curves from each lung were generated and clearance half-time (T1/2) of Tc-99m DTPA were calculated. T1/2 of whole lung was calculated as a mean of the T1/2 of left and right lung. The T1/2 values of Tc-99m DTPA clearance in the substance abusers were significantly decreased as compared to the control group with respective mean values of 28.86±8.44, and 62.14±26.12 min (p=0.001). It was seen Tc-99m DTPA clearance from lung was faster as the duration of substance abuse was increased. Tc-99m DTPA pulmonary clearance is markedly accelerated in the volatile substance abuse. This suggests that inhalant abuse of substance may produce abnormalities in pulmonary alveolo-capillary membrane function. (author)

  20. Effect of increased surface tension and assisted ventilation on /sup 99m/Tc-DTPA clearance

    International Nuclear Information System (INIS)

    Experiments were performed to determine the effects of conventional mechanical ventilation (CMV) and high-frequency oscillation (HFO) on the clearance of technetium-99m-labeled diethylenetriamine pentaacetate (/sup 99m/Tc-DTPA) from lungs with altered surface tension properties. A submicronic aerosol of /sup 99m/Tc-DTPA was insufflated into the lungs of anesthetized, tracheotomized rabbits before and 1 h after the administration of the aerosolized detergent dioctyl sodium sulfosuccinate (OT). Rabbits were ventilated by one of four methods: 1) spontaneous breathing; 2) CMV at 12 cmH2O mean airway pressure (MAP); 3) HFO at 12 cmH2O MAP; 4) HFO at 16 cmH2O MAP. Administration of OT resulted in decreased arterial PO2 (PaO2), increased lung wet-to-dry weight ratios, and abnormal lung pressure-volume relationships, compatible with increased surface tension. /sup 99m/Tc-DTPA clearance was accelerated after OT in all groups. The post-OT rate of clearance (k) was significantly faster (P less than 0.05) in the CMV at 12 cmH2O MAP [k = 7.57 +/- 0.71%/min (SE)] and HFO at 16 cmH2O MAP (k = 6.92 +/- 0.61%/min) groups than in the spontaneously breathing (k = 4.32 +/- 0.55%/min) and HFO at 12 cmH2O MAP (4.68 +/- 0.63%/min) groups. The clearance curves were biexponential in the former two groups. We conclude that pulmonary clearance of /sup 99m/Tc-DTPA is accelerated in high surface tension pulmonary edema, and this effect is enhanced by both conventional ventilation and HFO at high mean airway pressure

  1. The assessment of the patency of peritoneo-venous shunt with Tc99m-DTPA

    International Nuclear Information System (INIS)

    The patency of a LeVeen peritoneo-venous shunt was assessed with a radiopharmaceutical Tcsup(99m)-DTPA, Tcsup(99m)-sulphur colloid was used previously for this purpose Tcsup(99m)-DTPA was given intraperitoneally in three patients with ascites and the blood activity was determined. The radioactivity in the blood was found to be minimal and this was attributed to free pertechnetate. The kidneys and the bladder could not be demonstrated at 45 minutes. This also shows that the minimal activity in the blood was negligible for scintigraphic studies. The valve or the peritoneal tubing of the LeVeen shunt are sometimes occluded with a trombus or clot. So if the proximal part of the tube is demonstrated scintigraphically, it shows that the valve is functioning. But if the distal end of the tubing is occluded, this study will give a false-negative result. It can be claimed that if Tcsup(99m)-sulphur colloid is found in the blood, the shunt will be accepted as patent. The demonstration of the liver in 60 minutes after intraperitoneal injection shows the patency of the shunt precisely. But, demonstration of liver later than 60 min is not an evidence for a patent shunt. As seen with the shunt, after the intraperitoneal injection of Tcsup(99m)-DTPA, the tubing was demonstrated in 5 minutes and the kidneys and bladder in 15 minutes. This finding rules out any distal occlusion of the tubing of the shunt. Another indirect finding to assess the patency of the shunt is the increased background activity after 30 minutes in the chest. The advantages of using Tcsup(99m)-DTPA over Tcsup(99m) sulphur colloid are homogeneous distribution of the radiopharmaceutical in the ascitic fluid more rapid assessment of the patency of the shunt and lower radiation dose to the patient

  2. Gd-DTPA enhancement of the facial nerve in Ramsay Hunt's syndrome

    Energy Technology Data Exchange (ETDEWEB)

    Kato, Tsutomu; Yanagida, Masahiro; Yamauchi, Yasuo (Kansai Medical School, Moriguchi, Osaka (Japan)) (and others)

    1992-10-01

    A total of 21 MR images in 16 Ramsay Hunt's syndrome were evaluated. In all images, the involved side of peripheral facial nerve were enhanced in intensity after Gd-DTPA. However, 2 cases had recovered facial palsy when MR images were taken. Nine of 19 cases with the enhancement of internal auditory canal portion had vertigo or tinnitus. Thus, it was suggested that the enhancement of internal auditory canal portion and clinical feature are closely related. (author).

  3. Clinical diagnostic application of 111In-DTPA-octreotide scintigraphy in small cell lung cancer.

    Science.gov (United States)

    Vaccarili, M; Lococo, A; Fabiani, F; Staffilano, A

    2000-01-01

    Some years ago it was proved that a good percentage of small cell lung cancers, classified among cancers of the APUD system, produces somatostatin receptors that can be detected in vivo by scintigraphy with 111In-DTPA-octreotide. With the method in the whole body it is possible to identify the principal neoformation and the probable metastases. The authors present a study of 21 patients afflicted with small cell lung cancer diagnosed histologically. The study, carried out between January 1995 and December 1997, compared the radiologic iconography of the CT scan with the scintigraphic map obtained by a planar scintigraphy and in SPECT 1, 4 and 24-hr after iv injection of 110 MBq of 111In-DTPA-octreotide. The comparison was made with reference to the principal neoplasm and probable metastases. A scintigraphic study, a CT of restaging and a follow-up, done after 3 and 6 months of chemotherapy, on 15 patients with cancer that produces somatostatin receptors proved that the neoplasm sometimes regresses and sometimes progresses. In the latter case, it is possible to identify cerebral, mediastinal and hepatic metastases with the administration of 200 microg of octreotide 3 times a day for 7 days before the scintigraphy. In fact, the administration lowers background activity. The authors concluded that scintigraphy with 111In-DTPA-octreotide plays an important part in the study of patients afflicted with small cell lung cancer. Scintigraphy identifies the subgroups of patients who can be cured with somatostatin analogues together with chemotherapy. Scintigraphy presents a good sensibility in the re-staging and in the follow-up of patients who are treated, even though it is difficult to identify subdiaphragmatic metastases where liver, spleen and kidney show an increase in 111In-DTPA-octreotide. PMID:10939603

  4. Permeability of the bronchial mucosa to /sup 99m/Tc-DTPA in asthma

    International Nuclear Information System (INIS)

    Previous investigators, using 99mTc-DTPA aerosol as a marker to assess epithelial permeability in asthma, did not find an increased permeability in this group. However, they either failed to deliver the aerosol to the optimal site (bronchial mucosa, not alveoli) or failed to account for mucociliary clearance in analyzing their results. We studied 10 asthmatics and eight age-matched control subjects using a dosimeter (Spira-Elektra 2) and a carefully controlled breathing pattern to deliver aerosol to the subjects' airways. Two aerosols were delivered on separate days in each patient; 99mTc-DTPA aerosol, and 99mTc-HSA (human serum albumin), using similar breathing patterns to ensure reproducibility of the deposition pattern with the two aerosols. From measurements of retention versus time over a 1-h period, rate constants Ktot and Km were determined for the clearance of 99mTc-DTPA and 99mTc-HSA, respectively. By modelling the airways as a single compartment with two possible routes of clearance, we determined the permeability rate constant, Kp, as Ktot minus Km. There was no significant difference between Ktot in normal subjects and asthmatics; however, because of the slower mucociliary clearance in the asthmatic group, and the relative importance of mucociliary clearance in determining the washout of 99mTc-DTPA aerosol, there was a significant difference in airway permeability between the normal subjects and the asthmatics (t1/2 = 296 min +/- 141 SD and 126 min +/- 58, p less than 0.01, in normal subjects and asthmatics, respectively)

  5. Additional extrarenal abnormalities seen in Tc-99m DTPA renal flow study

    Energy Technology Data Exchange (ETDEWEB)

    Shih, Wei-Jen; Riley, C.; Domstad, P.A.; Pulmano, C.

    1988-04-01

    During /sup 99m/Tc-DTPA renal flow studies, extrarenal abnormalities have been found to include aortic abnormalities (aneurysm, ectasia, thrombosis, and abruptly decreased flow), splenic abnormalities (enlarged, small, or absent spleen), hepatic arterialization, and very slow circulation. In addition to the above abnormal findings, we add three more extrarenal pathologies that may be concomitantly found with renal flow study : pleural effusion(s), malignancy of the abdomen, and anemia and/or skeletal metastases.

  6. Design of Ion-Exchange Resins Through EDTA and DTPA Modified Ligands

    Directory of Open Access Journals (Sweden)

    2014-07-01

    Catechol, resorcinol, and their admixtures with EDTA and DTPA moieties were converted into polymeric resins by alkaline polycondensation with formaldehyde. The resins were characterized by FTIR spectroscopy, elemental analysis, ion-exchange capacity, and distribution coefficient (D for heavy metal and radionuclide such as Cs and Sr. 137Cs and 90Sr constitutes a major source of heat in nuclear waste streams and in regards to recent nuclear event their remediation in complex solution – sea water - represent an important issue.

  7. A triphenylamine-based benzoxazole derivative as a high-contrast piezofluorochromic material induced by protonation.

    Science.gov (United States)

    Xue, Pengchong; Chen, Peng; Jia, Junhui; Xu, Qiuxia; Sun, Jiabao; Yao, Boqi; Zhang, Zhenqi; Lu, Ran

    2014-03-11

    A triphenylamine-based benzoxazole derivative exhibits a low contrast piezofluorochromic behavior under external pressure, and a high-contrast fluorescence change induced by protonation can be observed.

  8. Development of [{sup 201}Tl](III)-DTPA-human polyclonal antibody complex for inflammation detection

    Energy Technology Data Exchange (ETDEWEB)

    Jalilian, A.R.; Kamali-Dehghan, M.; Kamrani, Y.Y. [Nuclear Research Center for Agriculture and Medicine, Karaj (Iran). Cyclotron and Nuclear Medicine Dept.; Khorrami, A.; Tavakoli, M.B. [Medical Sciences Univ. of Isfahan (Iran). Medical Physics and Engineering Dept.

    2007-07-01

    Thallium-201 (T{sub 1/2}=3.04 d) in Tl{sup +} form was converted to Tl{sup 3+} cation in presence of O{sub 3} in 6 M HCl controlled by RTLC/gel electrophoresis methods and used in the labeling of human polyclonal antibody (HIgG) after conjugation with freshly prepared cyclic DTPA-dianhydride. The best results of the conjugation were obtained by the addition of 1 mL of a HIgG pharmaceutical solution (5 mg/ml, in phosphate buffer, pH=7) to a glass tube pre-coated with DTPA-dianhydride (0.01 mg) at 25 C with continuous mild stirring for 30 min. The final isotonic [{sup 201}Tl](III)-DTPA-HIgG complex was checked by radio-TLC using several solvent systems to ensure the formation of only one species followed by filtration through a 0.22 {mu} filter (specific activity= 33.7 TBq/mM, radiochemical purity >95%). Preliminary bio-distribution studies in normal and inflammation-bearing rats were performed. The target/skin and target/blood ratios were 4 and 6 after 28 h respectively, showing the selectivity of the radiopharmaceutical for the inflammatory lesions. (orig.)

  9. Pulmonary clearance of 99mTc-DTPA aerosol in patients with progressive systemic scleroderma

    International Nuclear Information System (INIS)

    Alveolar epithelial permeability was assessed in 32 patients with progressive systemic scleroderma (PSS), using 99mTc-DTPA aerosol. Immediately after the inhalation of 99mTc-DTPA aerosol for 3 to 6 minutes under normal tidal breathing, lung was imaged sequentially for 30 minutes from the posterior by a gamma camera and exponential fitting was processed on the time activity curve. T1/2 (min) was used as a parameter for the evaluation of permeability of alveolar epithelium. Patients with collagen disease showed shorter T1/2 (T1/2=43.7±23.8 min) than the normal volunteers (T1/2=76.8±8.7 min). No significant difference was observed between patients with or without interstitial changes on the chest CT. Significant correlation was not observed between T1/2 and %VC or %DLco. In 8 cases, studies were repeated in the interval of 3 to 19 months. Improvement of T1/2 was seen in 4 cases, independent of CT findings. These results suggest that 99mTc-DTPA aerosol clearance study provides information independent from other lung examinations, and may be useful for the assessment of lung interstitial changes in patients with PSS. (author)

  10. Electrodynamic Analysis of Dissipative Electromagnetic Materials Based on Fractional Derivative

    Institute of Scientific and Technical Information of China (English)

    TAN Kang-Bo; LIANG Chang-Hong; DANG Xiao-Jie

    2007-01-01

    The generalized Lagrangian is defined in a dissipative electromagnetic medium on the basis of the combination of dynamical analysis and fractional derivative.Lorentz medium models are obtained by formulating relevant EulerLagrange equations.The invariance is obtained subsequently by investigating the invariance of time variation in the system,and then the relation between the related Hamiltonian and electromagnetic energy density is investigated.Canonical equations are obtained eventually.The electrodynamic interpretation on dissipative electromagnetic systems is revesled.

  11. Measurement of Regional and Global Pulmonary Clearance of 99mTc-DTPA (Demethylamitriptylene-Acetate): An Index of Alveolar Epithelial Permeability

    International Nuclear Information System (INIS)

    The main purpose of this study has been introduction of a new method for alveoli-capillary permeability evaluation. Many reports pointed out to the altered transit of soluble particles through this barrier. From pathophysiological aspect the main interest is the elucidation of permeability's alteration in different pulmonary pathology. We decided to use for lung epithelial permeability measurements 99mTc-DTPA inhaled aerosols and sequential assessment of its lung clearance. The aerosols were obtained using oxygen flow nebulizers with aerosols' generators Ultra Vent (Malinkrodt) and Venticis II (CIS bio international) that enabled as to get submicron particles. Oxygen flow between 9 and 11 liters per minute was used. Optimum images were obtained with 1480 MBq of inhaled aerosols at least 2 to 3 minutes. DTPA that was used for aerosols labeling had been produced in our Department and the results were compared with DTPA provided by CIS bio international. High correlation between both agents was proven. During the whole study ex tamper prepared radiopharmaceuticals were used and quality control was done using paper chromatography method. Acquisition was done in sitting position with gamma camera interfaced to a ADAC and Scintiview. The measurements lasted for 20 minutes. Data were stored on 64x64 matrices. Regions of interest over both lungs were drown and each one was divided in three segments: apical, medial, and basal. Using computer program curves of 99mTc-DTPA lung clearance were derived. From the obtained time activity curves half-time of the global and the regional lung clearance was assessed. In the control group comprised of 32 healthy volunteers (non-smokers) we had got values, used after works as reference range. Our normal values for global clearance are: 68±5,5 min. for left whole lung, 68,1±6,5 min for right whole lung, and 49±7,7 min for apical, 66,9±8 min for middle, and 75,9±6,4 min basal regional lung clearance, and they are in keeping with the

  12. Evaluation in vitro and in rats of161Tb-DTPA-octreotide, a somatostatin analogue with potential for intraoperative scanning and radiotherapy

    OpenAIRE

    De Jong, Marcel; Breeman, Wouter; Bernard, Bert; Rolleman, Edgar; Hoflande, Leo; Visser, Ton; Setyono-Han, Buddy; Willem BAKKER; Pluijm, M.; Krenning, Eric

    1995-01-01

    textabstractThe characteristics of terbium-161 diethylene triamine penta-acetic acid (DTPA) labelled octreotide with respect to specific binding to somatostatin (octreotide) receptors on rat brain cortex membranes, biological activity, uptake and excretion by isolated perfused rat livers and metabolism in vivo in normal and tumour-bearing rats were determined and compared to those of indium-111 DTPA-octreotide. The results of the binding studies demonstrate that161Tb-DTPA-octreotide is a high...

  13. Gadolinium MRI Contrast Agents Based on Triazine Dendrimers: Relaxivity and In Vivo Pharmacokinetics

    OpenAIRE

    Lim, Jongdoo; Turkbey, Baris; Bernardo, Marcelino; Bryant, L. Henry; Garzoni, Matteo; Pavan, Giovanni M.; Nakajima, Takahito; Choyke, Peter L; Simanek, Eric E; Kobayashi, Hisataka

    2012-01-01

    Four gadolinium (Gd)-based macromolecular contrast agents, G3-(Gd-DOTA)24, G5-(Gd-DOTA)96, G3-(Gd-DTPA)24, and G5-(Gd-DTPA)96, were prepared that varied in the size of dendrimer (generation three and five), the type of chelate group (DTPA or DOTA), and the theoretical number of metallated chelates (24 and 96). Synthesis relied on a dichlorotriazine derivatized with a DOTA or DTPA ligand that was incorporated into the dendrimer and ultimately metallated with Gd ions. Paramagnetic characteristi...

  14. Development of {sup 68}Ga-labelled DTPA galactosyl human serum albumin for liver function imaging

    Energy Technology Data Exchange (ETDEWEB)

    Haubner, Roland [Innsbruck Medical University, Department of Nuclear Medicine, Innsbruck (Austria); Medizinische Universitaet Innsbruck, Universitaetsklinik fuer Nuklearmedizin, Innsbruck (Austria); Vera, David R.; Farshchi-Heydari, Salman [University of California, Department of Radiology, School of Medicine, and the UCSD Molecular Imaging Program, San Diego, CA (United States); Helbok, Anna; Rangger, Christine; Putzer, Daniel; Virgolini, Irene J. [Innsbruck Medical University, Department of Nuclear Medicine, Innsbruck (Austria)

    2013-08-15

    The hepatic asialoglycoprotein receptor is responsible for degradation of desialylated glycoproteins through receptor-mediated endocytosis. It has been shown that imaging of the receptor density using [{sup 99m}Tc]diethylenetriamine pentaacetic acid (DTPA) galactosyl human serum albumin ([{sup 99m}Tc]GSA) allows non-invasive determination of functional hepatocellular mass. Here we present the synthesis and evaluation of [{sup 68}Ga]GSA for the potential use with positron emission tomography (PET). Labelling of GSA with {sup 68}Ga was carried out using a fractionated elution protocol. For quality control thin-layer chromatography (TLC), high-performance liquid chromatography (HPLC) and size exclusion chromatography (SEC) techniques were evaluated. Stability of [{sup 68}Ga]GSA was studied in phosphate-buffered saline (PBS) and human serum. For in vivo evaluation [{sup 68}Ga]GSA distribution in Lewis rats was compared with [{sup 99m}Tc]GSA by using a dual isotope protocol. PET and planar imaging studies were performed using the same scaled molar dose of [{sup 68}Ga]GSA and [{sup 99m}Tc]GSA. Time-activity curves (TAC) for heart and liver were generated and corresponding parameters calculated (t50, t90). [{sup 68}Ga]GSA can be produced with high radiochemical purity. The best TLC methods for determining potential free {sup 68}Ga include 0.1 M sodium citrate as eluent. None of the TLC methods tested were able to determine potential colloids. This can be achieved by SEC. HPLC confirmed high radiochemical purity (>98 %). Stability after 120 min incubation at 37 C was high in PBS (>95 % intact tracer) and low in human serum ({proportional_to}27 % intact tracer). Biodistribution studies simultaneously injecting both tracers showed comparable liver uptake, whereas activity concentration in blood was higher for [{sup 68}Ga]GSA compared to [{sup 99m}Tc]GSA. The [{sup 99m}Tc]GSA TACs exhibited a small degree of hepatic metabolism compared to the [{sup 68}Ga]GSA curves. The mean

  15. Platelet-Rich Blood Derivatives for Stem Cell-Based Tissue Engineering and Regeneration

    NARCIS (Netherlands)

    Masoudi, E.A.; Ribas, J.; Kaushik, G.; Leijten, J.C.H.; Khademhosseini, A.

    2016-01-01

    Platelet-rich blood derivatives have been widely used in different fields of medicine and stem cell-based tissue engineering. They represent natural cocktails of autologous growth factors, which could provide an alternative for recombinant protein-based approaches. Platelet-rich blood derivatives, s

  16. The discrete variational derivative method based on discrete differential forms

    Science.gov (United States)

    Yaguchi, Takaharu; Matsuo, Takayasu; Sugihara, Masaaki

    2012-05-01

    As is well known, for PDEs that enjoy a conservation or dissipation property, numerical schemes that inherit this property are often advantageous in that the schemes are fairly stable and give qualitatively better numerical solutions in practice. Lately, Furihata and Matsuo have developed the so-called “discrete variational derivative method” that automatically constructs energy preserving or dissipative finite difference schemes. Although this method was originally developed on uniform meshes, the use of non-uniform meshes is of importance for multi-dimensional problems. On the other hand, the theories of discrete differential forms have received much attention recently. These theories provide a discrete analogue of the vector calculus on general meshes. In this paper, we show that the discrete variational derivative method and the discrete differential forms by Bochev and Hyman can be combined. Applications to the Cahn-Hilliard equation and the Klein-Gordon equation on triangular meshes are provided as demonstrations. We also show that the schemes for these equations are H1-stable under some assumptions. In particular, one for the nonlinear Klein-Gordon equation is obtained by combination of the energy conservation property and the discrete Poincaré inequality, which are the temporal and spacial structures that are preserved by the above methods.

  17. An Adaptive Derivative-based Method for Function Approximation

    Energy Technology Data Exchange (ETDEWEB)

    Tong, C

    2008-10-22

    To alleviate the high computational cost of large-scale multi-physics simulations to study the relationships between the model parameters and the outputs of interest, response surfaces are often used in place of the exact functional relationships. This report explores a method for response surface construction using adaptive sampling guided by derivative information at each selected sample point. This method is especially suitable for applications that can readily provide added information such as gradients and Hessian with respect to the input parameters under study. When higher order terms (third and above) in the Taylor series are negligible, the approximation error for this method can be controlled. We present details of the adaptive algorithm and numerical results on a few test problems.

  18. Antioxidant activity of N-acetyl-glucosamine based thiazolidine derivative

    Institute of Scientific and Technical Information of China (English)

    Li Chunlei; Yang Yan; Han Baoqin; Liu Wanshun

    2007-01-01

    N-acetyl-glucosamine,the monomer of chitin,was cyclo-condensed with L-cysteine to prepare thiazolidine derivative:2-N-acetyl-glucosamine-thiazolidine-4(R)-carboxylic acid(GlcNAcCys).The stability of GlcNAcCys was evaluated by high performance liquid chromatography(HPLC)measurement.The results showed that GlcNAcCys Was more stable than other TCA derivatives,especially in alkaline condition.The direct in vitro antioxidative properties of GlcNAcCys were investigated by using UV radiation-induced lipid peroxidation(LPO)in mitochondria and nuclei and.OH-induced LPO in red blood cell (RBC)ghosts models.UV radiation caused dose-dependent LPO in both mitochondria and nuclei,this effect Was catalvzed by addition of Fd2+ while prevented by co-incubation with GlcNAcCys.When nuclei and mitochondria Was treated with 100μl,300μl,500μl of GlcNAcCys and co-incubated at 37℃ for 30min,LPO was decreased to 96%,72%,68%in nuclei and 95%,72%,68% in mitochondria when compared to the UV radiation group respectively.Hydroxyl radicals(.OH)generated by Fenton reaction induced LPO in RBC ghosts.Pretreatment of RBC ghosts with GlcNAcCys could induce antioxidant RBC ghosts and inhibit concentration-dependent malondialdehyde(MDA)formation in antioxidant RBC ghosts.Its inhibition percent Was 14%,35%,36%,42%at 10,20,30,40ms/ml respectively.In a conclusion,the data suggest that GlcNAcCys has antioxidant ability and can significantly inhibit lipid peroxidation in biological samples tested in vitro.

  19. MRI of osteochontritis dissecans of the knee joint after i. v. gadolinium-DTPA. Magnetresonanztomographie der Osteochondrosis dissecans des Kniegelenkes nach intravenoeser Gadolinium-DTPA-Gabe

    Energy Technology Data Exchange (ETDEWEB)

    Adam, G. (Klinik fuer Radiologische Diagnostik, RWTH Aachen (Germany)); Neuerburg, J. (Klinik fuer Radiologische Diagnostik, RWTH Aachen (Germany)); Peiss, J. (Klinik fuer Radiologische Diagnostik, RWTH Aachen (Germany)); Bohndorf, K. (Klinik fuer Radiologische Diagnostik, RWTH Aachen (Germany)); Guenther, R.W. (Klinik fuer Radiologische Diagnostik, RWTH Aachen (Germany))

    1994-05-01

    20 patients with osteochondritis dissecans were studied by MRI over a period of 3-34 monts in order to observe healing and determine the viability of the loose fragment. After intravenous contrast, there was increased signal intensity at the junction between the fragment and the epiphyseal bed on T[sub 2]-weighted sequences; this was interpreted as fibrous granulation tissue surrounding the fragment. The fragments themselves also showed increased signal intensity after Gadolinium-DTPA. Intravenous Gadolinium allows accurate evaluation of the junctional zone between the fragment and the epiphyseal bed by demonstrating fibrous, vascularised granulation tissue. This can be distinguished from synovial fluid, and it also demonstrates viability of the fragment. (orig.)

  20. Value of Tc99m-DTPA alveolar permeability in lung involvement detection of patients with HIV infection

    International Nuclear Information System (INIS)

    We studied 35 HIV patients in order to know the value of Tc99mDTPA in the assessment of pulmonary lung involvement, especially pneumocystis carinii (PC) infection. Lung DTPA clearance measures increased alveolar permeability. Twenty patients with respiratory symptoms were included, 4 with systemic symptoms and also 11 asymptomatics, with similar immune condition (CD4 lymphocytes <400) as a control group. Smoking habit was suspended prior the test. Clinical follow up, chest film, induced sputum and/or fibrobronchoscopy were obtained. There was histological confirmation of PC presence or absence in 16 symptomatics and 3 asymptomatics. DTPA sensitivity for PC detection was 78%, specificity 40% and accuracy 58%; the values were 85%, 60% and 79%, respectively, for inflammatory lung processes. There were 4/6 cases false positive for PC detection with respiratory features explaining DTPA abnormalities. Concluding, Tc99m-DTPA is sensitive but not specific for detecting PC pneumonia but its value is higher for pulmonary inflammatory processes (Au)

  1. The exploratory development of A self-made oral Gd-DTPA solution for low field MRCP in children

    International Nuclear Information System (INIS)

    Objective: To evaluate the value of ingestion self-made Gd-DTPA solutin for low field magnetic resonance cholangiopancreatography (MRCP) (0.35T) in children. Methods: Phantom experiments were performed to select the optimal concentration of Gd-DTPA to be used as an oral negative contrast agent in low field MRCP. Twenty children suspected of celiac disease were performed with MRCP before and after ingestion Gd-DTPA solution. Signal intensity measurements of gastroduodenal lumens, pancreatobiliary ducts, and image quality scores were obtained systematically before and after contrast ingestion. Results: The selected Gd-DTPA concentration was 1.992 mmol/L. Ingestion of 80 ml solution eliminated or suppressed efficiently the gastroduodenal signal intensity, improving the MRCP image quality scores (P<0.01) significantly in children. Conclusion: 1.992 mmol/L self-made Gd-DTPA solutin can suppress or eliminate water signals from gastrointestinal tract and improve the quality of low field MRCP image in children. (authors)

  2. Evaluation of Ferrocene Derivatives as Burn Rate Modifiers in AP/HTPB-Based Composite Propellants

    OpenAIRE

    G. M. Gore; K. R. Tipare; R. G. Bhatewara; U.S. Prasad; Manoj Gupta; S. K. Mane

    1999-01-01

    Some ferrocene derivatives like 2,4-dinitrophenylhydrazine derivative of acetyl ferrocene, 1-pyrrolidinylmethyl ferrocene, di-ter-butyl ferrocene and 1,3-diferrocenyl-l-butene (DFB) have been synthesised and characterised by infrared, nuclear magnetic resonance, ultraviolet, iron content, etc. To study the effect of their incorporation on performance, ammonium perchlorate/hydroxyl-terminated polybutadiene-based composite propellants containing these derivatives have been prepared and studied ...

  3. Intraoperative tumour detection using 111In-DTPA-D-Phe1-octreotide and a scintillation detector

    International Nuclear Information System (INIS)

    Intraoperative tumour detection has been used in many applications. The examined tumour forms have varied and different detector systems and radiopharmaceuticals have also been used. The aim of this study was to evaluate and compare the ability of an NaI(Tl) scintillation detector to detect primary tumours and metastases in patients with different endocrine tumour types (e.g. carcinoid tumours, endocrine pancreatic tumours and thyroid tumours) and in patients with breast carcinoma or benign thyroid lesions, on the basis of their somatostatin receptor expression after i.v. injection of 111In-DTPA-D-Phe1-octreotide. Thirty patients were injected with 111In-DTPA-D-Phe1-octreotide intravenously. Scintigraphic images were taken 1 day after injection of the radiopharmaceutical, and surgery was performed 1-7 days post injection. An NaI(Tl) scintillation detector was used for intraoperative tumour detection. Tissue samples were collected during surgery for determination of 111In activity concentration and histopathological examination. The scintigraphic images were positive in 29 out of 30 patients. Intraoperative tumour detection was successful in 43 of 66 collected biopsies: 10 out of 11 for carcinoid tumours, 7 out of 10 for medullary thyroid carcinoma (MTC) and 14 out of 22 for breast cancer. On the basis of our findings we conclude that intraoperative tumour detection with 111In-DTPA-D-Phe1-octreotide using this NaI(Tl) detector can be successful especially for carcinoid tumours and endocrine pancreatic tumours, due to the relatively high activity concentrations in these tumour types, but is less successful in other forms of thyroid cancer, including MTC, and breast cancer. For successful intraoperative detection, the detector characteristics are also very important, and further improvement of the detector systems is required to increase the sensitivity and specificity. (orig.)

  4. Lung clearance of 99mTc-DTPA in systemic lupus erythematosus

    Directory of Open Access Journals (Sweden)

    P.T.R. Dalcin

    2002-06-01

    Full Text Available The early demonstration of lung involvement in systemic lupus erythematosus (SLE patients is a difficult but important task. In the present study we attempted to identify abnormalities in pulmonary clearance of 99mTc-DTPA in SLE, correlating their clearance data with clinical findings and disease activity. Forty-six consecutive SLE patients with and without active disease (LACC score and 30 normal volunteers were studied. All subjects were submitted to pulmonary scintigraphy with 99mTc-DTPA to evaluate the pulmonary clearance, and to a chest X-ray, and SLE patients were submitted to tests of disease activity, spirometry, arterial blood gases and tests to assess acute-phase proteins. Pulmonary clearance was faster in SLE patients with active disease when compared to normal controls [half-life of 67.04 min (51.52-82.55 min in active SLE versus 85.87 min (78.85-92.87 min in controls, P<0.05] and there was a higher frequency of abnormal clearance rates in patients with active disease (11 of 26 patients, 42.3% when compared with SLE patients without disease activity (2 of 20 patients, 10% (P = 0.04. A significant correlation was observed between the clearance rates and cough (P<0.05, but not between the clearance rates and dyspnea symptoms or radiological findings, duration of SLE disease, antinuclear antibody titers and patterns, C-reactive protein or anti-double stranded DNA antibodies. We conclude that the pulmonary clearance of 99mTc-DTPA is increased in SLE patients with active disease.

  5. Lung clearance of 99m Tc- DTPA in systemic lupus erythematous

    International Nuclear Information System (INIS)

    The early demonstration of lung involvement in systemic lupus erythematosus (SLE) patients is a difficult but important task. In the present study we attempted to identify abnormalities in pulmonary clearance of 99 mTc-DTPA in SLE, correlating their clearance data with clinical findings and disease activity. Forty-six consecutive SLE patients with and without active disease (LACC score) and 30 normal volunteers were studied. All subjects were submitted to pulmonary scintigraphy with 99 mTc-DTPA to evaluate the pulmonary clearance, and to a chest X-ray, and SLE patients were submitted to tests of disease activity, spirometry, arterial blood gases and tests to assess acute-phase proteins. Pulmonary clearance was faster in SLE patients with active disease when compared to normal controls [half-life of 67.04 min (51.52-82.55 min) in active SLE versus 85.87 min (78.85-92.87 min) in controls, P<0.05] and there was a higher frequency of abnormal clearance rates in patients with active disease (11 of 26 patients, 42.3%) when compared with SLE patients without disease activity (2 of 20 patients, 10%) (P = 0.04). A significant correlation was observed between the clearance rates and cough (P<0.05), but not between the clearance rates and dyspnoea symptoms or radiological findings, duration of SLE disease, antinuclear antibody titers and patterns, C-reactive protein or anti-double stranded DNA antibodies. We conclude that the pulmonary clearance of 99 m Tc-DTPA is increased in SLE patients with active disease. (author)

  6. Effect of inspiratory resistance and PEEP on /sup 99m/Tc-DTPA clearance

    International Nuclear Information System (INIS)

    Experiments were performed to determine the effect of markedly negative pleural pressure (Ppl) or positive end-expiratory pressure (PEEP) on the pulmonary clearance (k) of technetium-99m-labeled diethylenetriaminepentaacetic acid (/sup 99m/Tc-DTPA). A submicronic aerosol containing /sup 99m/Tc-DTPA was insufflated into the lungs of anesthetized intubated sheep. In six experiments k was 0.44 +/- 0.46% (SD)/min during the initial 30 min and was unchanged during the subsequent 30-min interval [k = 0.21 +/- 12%/min] when there was markedly increased inspiratory resistance. A 3-mm-diam orifice in the inspiratory tubing created the resistance. It resulted on average in a 13-cmH2O decrease in inspiratory Ppl. In eight additional experiments sheep were exposed to 2, 10, and 15 cmH2O PEEP (20 min at each level). During 2 cmH2O PEEP k = 0.47 +/- 0.15%/min, and clearance increased slightly at 10 cmH2O PEEP [0.76 +/- 0.28%/min, P less than 0.01]. When PEEP was increased to 15 cmH2O a marked increase in clearance occurred [k = 1.95 +/- 1.08%/min, P less than 0.001]. The experiments demonstrate that markedly negative inspiratory pressures do not accelerate the clearance of /sup 99m/Tc-DTPA from normal lungs. The effect of PEEP on k is nonlinear, with large effects being seen only with very large increases in PEEP

  7. Lung clearance of {sup 99m} Tc- DTPA in systemic lupus erythematous

    Energy Technology Data Exchange (ETDEWEB)

    Dalcin, P.T.R; Barreto, S.S.M.; Xavier, R.M.; Brenol, J.C.T. [Rio Grande do Sul Univ., Porto Alegre, RS (Brazil). Hospital de Clinicas. Dept. de Medicina Interna; Cunha, R.D.; Marroni, B.J. [Rio Grande do Sul Univ., Porto Alegre, RS (Brazil). Hospital de Clinicas. Servico de Medicina Nuclear]. E-mail: rxavier@hcpa.ufrgs.br

    2002-06-01

    The early demonstration of lung involvement in systemic lupus erythematosus (SLE) patients is a difficult but important task. In the present study we attempted to identify abnormalities in pulmonary clearance of 99 mTc-DTPA in SLE, correlating their clearance data with clinical findings and disease activity. Forty-six consecutive SLE patients with and without active disease (LACC score) and 30 normal volunteers were studied. All subjects were submitted to pulmonary scintigraphy with 99 mTc-DTPA to evaluate the pulmonary clearance, and to a chest X-ray, and SLE patients were submitted to tests of disease activity, spirometry, arterial blood gases and tests to assess acute-phase proteins. Pulmonary clearance was faster in SLE patients with active disease when compared to normal controls [half-life of 67.04 min (51.52-82.55 min) in active SLE versus 85.87 min (78.85-92.87 min) in controls, P<0.05] and there was a higher frequency of abnormal clearance rates in patients with active disease (11 of 26 patients, 42.3%) when compared with SLE patients without disease activity (2 of 20 patients, 10%) (P = 0.04). A significant correlation was observed between the clearance rates and cough (P<0.05), but not between the clearance rates and dyspnoea symptoms or radiological findings, duration of SLE disease, antinuclear antibody titers and patterns, C-reactive protein or anti-double stranded DNA antibodies. We conclude that the pulmonary clearance of 99 m Tc-DTPA is increased in SLE patients with active disease. (author)

  8. Biobased Epoxy Nanocomposites Derived from Lignin-Based Monomers.

    Science.gov (United States)

    Zhao, Shou; Abu-Omar, Mahdi M

    2015-07-13

    Biobased epoxy nanocomposites were synthesized based on 2-methoxy-4-propylphenol (dihydroeugenol, DHE), a molecule that has been obtained from the lignin component of biomass. To increase the content of hydroxyl groups, DHE was o-demethylated using aqueous HBr to yield propylcatechol (DHEO), which was subsequently glycidylated to epoxy monomer. Optimal conditions in terms of yield and epoxy equivalent weight were found to be 60 °C with equal NaOH/phenolic hydroxyl molar ratio. The structural evolution from DHE to cured epoxy was followed by (1)H NMR and Fourier transform infrared spectroscopy. The nano-montmorillonite modified DHEO epoxy exhibited improved storage modulus and thermal stability as determined from dynamic mechanical analysis and thermogravimetric analysis. This study widens the synthesis routes of biobased epoxy thermosets from lignin-based molecules. PMID:26135389

  9. Tc-99m DTPA aerosol lung scintigraphy in patients requiring mechanical ventilation

    International Nuclear Information System (INIS)

    Xenon ventilation images are of limited diagnostic value in intubated patients since only posterior views can be readily obtained. In this study, Mallinckrodt Tc-99m DTPA aerosol was evaluated for its ability to provide six ventilation projections comparable to MAA perfusion images. Earlier particles have had the drawback to impacting in either ventilator tubing or the patient's major bronchi, limiting clinical utility. Nine patients were studied. Eight had good aerosol distribution without significant particle impaction. In the remaining patient, decreased aeration and holdup of activity beyond the area of impaction was confirmed by xenon ventilation images. Comparable ventilation and perfusion projections were obtained in every patient studied

  10. Multiple myeloma: Screening by Gd-DTPA enhanced opposed-phase gradient-echo MR imaging

    International Nuclear Information System (INIS)

    MR imaging was performed in 19 patients with proven multiple myeloma. Both plain and Gd-DTPA enhanced in-phase and opposed-phase gradient-echo techniques were used (0.1 mmol Magnevist/kg body weight). Plain, opposed-phase imaging demonstrated more lesions than plain in-phase imaging (35 vs. 16); enhanced opposed-phase imaging demonstrated more lesions than plain opposed-phase and enhanced in-phase imaging (47 vs. 35 and 17 lesions). These results suggest that enhanced opposed-phase images which have a high contrast between normal and infiltrated bone marrow are especially suited for MR screening in multiple myeloma. (orig.)

  11. Gastrointestinal scintigraphy with 99mTc-DTPA labeled tablets in fed and fasting subjects

    International Nuclear Information System (INIS)

    The in vitro and in vivo dissolution of a sustained release theophylline formulation labeled with 99mTc-diethyltriaminepentaacetic acid (DTPA) has been monitored in six subjects with a scintillation camera. The study was performed in fasting conditions and was repeated after ingestion of a standardized meal. Results showed that the presence of food in the stomach dramatically increased the oesoduodenal transit time of the tablet (74±27 min vs 352±77 min, P<0.001) but did not modify the biodisponibility of theophylline. This study is another example when scintigraphy can be of definite value in pharmacokinetics. (orig.)

  12. Extrarenal abnormalities in Tc-99m-DTPA renal blood flow studies

    Energy Technology Data Exchange (ETDEWEB)

    Shih, W.J.; Domstad, P.A.; DeLand, F.H.

    1985-01-01

    The authors observed extrarenal abnormalities during renal flow scintigraphy and retrospectively reviewed 90 patient studies to determine the types and frequencies of such abnormal findings. For each routine Tc-99m-DTPA renal flow study, they obtained nine 2-second sequential images, which included the heart, abdominal aorta, spleen and kidneys. Eighty abnormalities, observed in 62 patients, were divided into three categories: aortic, 37 cases; splenic, 40 cases; and miscellaneous, 3 cases. Other correlative studies including Tc-99m sulfur colloid-spleen scintigraphy, ultrasonography (US), CT, aortography, and surgical and/or autopsy findings were available for corroboration in 56 of 80 lesions.

  13. High sensitivity of the in vivo detection of somatostatin receptors by 111Indium (DTPA-Octreotide)-scintigraphy in meningioma patients

    International Nuclear Information System (INIS)

    The recent availability of isotope-labelled somatostatin analogues has allowed one to detect somatostatin receptors in normal tissue as well as in endocrine or non-endocrine cranial tumours. The purpose of the present study was to establish the value of somatostatin receptor scintigraphy using an 111indium-labelled somatostatin analogue, octreotide, in the diagnostic work-up of meningioma patients. Twenty-two patients (16 women, 6 men, aged from 19-70 years) with newly diagnosed, residual or recurrent cranial meningiomas were examined. 111indium-labelled DTPA-octreotide was injected i.v.. Planar and tomographic images were obtained with a gamma camera 4-6, and 24 hours after injection. In all of the meningiomas studies a high density of somatostatin receptor was detected by scintigraphy. No false negative test result was found. Due to this, a 100% predictive value of a negative test was calculated. However, when the tumours were taken in culture differing staining intensity could be seen in the light- and electron microscopic level even on individual cells of a single culture when silver intensified somatostatin-gold was used as ligand. We conclude, that in vivo somatostatin receptor scintigraphy may aid in the pre-operative differential diagnosis of skull base tumours

  14. Analytical Derivation: An Epistemic Game for Solving Mathematically Based Physics Problems

    Science.gov (United States)

    Bajracharya, Rabindra R.; Thompson, John R.

    2016-01-01

    Problem solving, which often involves multiple steps, is an integral part of physics learning and teaching. Using the perspective of the epistemic game, we documented a specific game that is commonly pursued by students while solving mathematically based physics problems: the "analytical derivation" game. This game involves deriving an…

  15. Disk-like Tiles Derived from Complex Bases

    Institute of Scientific and Technical Information of China (English)

    Jun LUO; Zuo Ling ZHOU

    2004-01-01

    For each positive integer k, the radix representation of the complex numbers in the base-k + i gives rise to a lattice self-affine tile Tk in the plane, which consists of all the complex numbers that can be expressed in the form ∑j≥1 dj(-k + i)-j, where dj ∈ {0, 1,2,……,k2}. We prove that Tk is homeomorphic to the closed unit disk {z ∈ C: |z| ≤ 1} if and only if k ≠ 2.

  16. Circulatory kinetics of intravenously injected {sup 238}Pu(IV) citrate and {sup 14}C-CaNa{sub 3}-DTPA in mice: Comparison with rat, dog, and Reference Man

    Energy Technology Data Exchange (ETDEWEB)

    Durbin, P.W.; Kullgren, B.; Schmidt, C.T. [Lawrence Berkeley National Lab., CA (United States)

    1997-02-01

    New ligands for in vivo chelation of Pu(IV) are being synthesized and evaluated in mice for efficacy and toxicity. Biokinetic studies of the new ligands, CaNa{sub 3}-DTPA, and Pu(IV) are major components of those investigations. Young adult female mice were injected intravenously (iv) with {sup 3}H-inulin, {sup 14}C-CaNa{sub 3}-DTPA, or {sup 238}Pu(IV) citrate to provide base- line data for plasma clearance, tissue uptake, and excretion rates and to determine the dilution volume (VOD) and renal clearance rate (RC) of filterable substances. Published plasma clearance data in Reference Man, dog, and rat were collected. Based on combined data for {sup 3}H-inulin and {sup 14}C-CaNa{sub 3}-DTPA, VOD = 17% of body weight and RC = 18 mL kg{sup -1} min{sup -1} for mice. Retention of {sup 14}C-CaNa{sub 3}-DTPA in the four species is proportional to body weight and inversely proportional to RC: Integrals of the retention of {sup 14}C-CaNa{sub 3}-DTPA from R(t) = 1.0 to R(t) = 0.05 are 108, 43, 28, and 10 DF min, respectively, for Reference Man, dog, rat, and mouse. Clearances of iv-injected Pu(IV) citrate from plasma are in the same order: The plasma curve integrals from injection to 1440 min are 840, 640, 280, and 67 DF min, respectively, for Reference Man, dog, rat, and mouse. In mice, a large fraction of newly injected Pu(IV) is rapidly transferred to the interstitial water of bulk soft tissue (excluding liver and kidneys), from which it is cleared at the same rate as from the plasma. Rapid plasma clearance, escape into interstitial water (22%ID at 20 min), significant early urinary excretion (8%ID in 12 h), and prompt deposition in liver and skeleton (complete in 12 h) are evidence of inefficient binding to plasma protein of newly injected Pu(IV) in mice. Slow plasma clearance, little early urinary excretion, and delayed deposition in liver and skeleton reflect more efficient binding of newly injected Pu(IV) in Reference Man and dog. 39 refs., 6 figs., 3 tabs.

  17. Evaluation of (177)Lu-CHX-A''-DTPA-Bevacizumab as a radioimmunotherapy agent targeting VEGF expressing cancers.

    Science.gov (United States)

    Kameswaran, Mythili; Pandey, Usha; Gamre, Naresh; Vimalnath, K V; Sarma, Haladhar Dev; Dash, Ashutosh

    2016-08-01

    This study aimed at the preparation and evaluation of (177)Lu-CHX-A''-DTPA-Bevacizumab for targeting VEGF over-expressing cancers. Bevacizumab conjugated to p-NCS-Bn-CHX-A''-DTPA was radiolabeled with (177)Lu. The radioimmunoconjugate characterized by SE-HPLC exhibited radiochemical purity of 98.0±0.6%. In vitro stability was retained upto 4 days at 37°C. In vitro cell binding studies showed good uptake by VEGF expressing U937 tumor cells. Biodistribution studies in melanoma model showed significant uptake and retention of (177)Lu-CHX-A''-DTPA-Bevacizumab in tumor with reduction in uptake in presence of cold Bevacizumab confirming its specificity to VEGF.

  18. The effect of airway deposition on the assessment of lung injury by 99mTc-DTPA clearance

    International Nuclear Information System (INIS)

    The 99mTc-DTPA aerosol inhalation method permits detection of pulmonary epithelial damage. We investigated one of several problems, airway deposition of inhaled aerosol, on the assessment of pulmonary epithelial permeability in healthy nonsmokers and patients with interstitial lung diseases. We used the rate constant of pulmonary 99mTc-DTPA clearance curve, k, as a parameter of the epithelial permeability. The alveolar-peripheral airway deposition of aerosol was estimated by the duplicated inhalation method, which we newly developed. The mean k in patients with interstitial lung disease (2.52±0.72%/min, n=8; pc) was higher in patients with interstitial lung disease (4.08±1.63%/min; pc in both groups (pc obtained among the subjects (r=0.951; p99mTc-DTPA aerosol inhalation method although the correction was significant in the individual subjects. (author)

  19. A New Fluorescent Sensor Based on Bisindolizine Derivative.

    Science.gov (United States)

    I, Anitha; M, Sheela Gopal; Thomas, Divya

    2016-03-01

    A fluorescent sensor based on 1,2,1',2'- Tetra(methoxycarbonyl)- 3,3'- bis(p-methylbenzoyl)- 7,7'-bisindolizine (MBI) showing excellent selectivity towards Fe(3+) ions was developed. Under optimized experimental conditions, the fluorescence intensity of 1,2,1',2'- Tetramethoxycarbonyl- 3,3'- bis(p-methylbenzoyl)- 7,7'-bisindolizine was quenched linearly by Fe(3+) ions in the range of 2.00 × 10(-2) to 4.76 × 10(-3) M. The limit of detection was found to be 3.17 × 10(-3) M. The mechanism for quenching was investigated. The developed sensor was applied for the determination of Fe(3+) in pharmaceutical samples. PMID:26758054

  20. Gd-EOB-DTPA-enhanced magnetic resonance images of hepatocellular carcinoma: correlation with histological grading and portal blood flow

    International Nuclear Information System (INIS)

    To retrospectively investigate enhancement patterns of hepatocellular carcinoma (HCC) and dysplastic nodule (DN) in the hepatobiliary phase of gadolinium-ethoxybenzyl-diethylenetriamine (Gd-EOB-DTPA)-enhanced MRI in relation to histological grading and portal blood flow. Sixty-nine consecutive patients with 83 histologically proven HCCs and DNs were studied. To assess Gd-EOB-DTPA uptake, we calculated the EOB enhancement ratio, which is the ratio of the relative intensity of tumorous lesion to surrounding nontumorous area on hepatobiliary phase images (post-contrast EOB ratio) to that on unenhanced images (pre-contrast EOB ratio). Portal blood flow was evaluated by CT during arterial portography. Post-contrast EOB ratios significantly decreased as the degree of differentiation declined in DNs (1.00 ± 0.14) and well, moderately and poorly differentiated HCCs (0.79 ± 0.19, 0.60 ± 0.27, 0.49 ± 0.10 respectively). Gd-EOB-DTPA uptake, assessed by EOB enhancement ratios, deceased slightly in DNs and still more in HCCs, while there was no statistical difference in the decrease between different histological grades of HCC. Reductions in portal blood flow were observed less frequently than decreases in Gd-EOB-DTPA uptake in DNs and well-differentiated HCCs. Reduced Gd-EOB-DTPA uptake might be an early event of hepatocarcinogenesis, preceding portal blood flow reduction. The hepatobiliary phase of Gd-EOB-DTPA-enhanced MRI may help estimate histological grading, although difficulties exist in differentiating HCCs from DNs. (orig.)

  1. Spatial heterogeneity of DTPA-extractable zinc in cultivated soils induced by city pollution and land use

    Institute of Scientific and Technical Information of China (English)

    JIANG Yong; LIANG Wenju; WEN Dazhong; ZHANG Yuge; CHEN Wenbo

    2005-01-01

    The spatial heterogeneity of DTPA-extractable zinc in the cultivated soils of Shenyang suburbs in Liaoning Province of China was investigated, and its map was drawn by the methods of geostatistics combined with geographic information system. The data of soil DTPA-extractable zinc fitted normal distribution after logarithm transformation, and its semivariogram fitted a spherical model. The semivariogram indicated that the spatial dependence of soil DTPA-extractable zinc content was moderate, with the spatial dependence range of 1.69 km and the fractal dimension of 1.96. Stochastic factors contributed to 49.9% of the spatial variability, while structural factors contributed to 50.1% of it. The spatial heterogeneity of soil DTPA-extractable zinc shown by a kriged interpolation map was deeply influenced by stochastic factors such as city pollution, land use pattern and crop distributions. For example, the average content of Zn in vegetable garden soils was 2.5-4 times as much as in their originated soils, and was lower in paddy soils than in their originated soils. The areas with a higher content of soil DTPA-extractable zinc appeared in the near suburbs and the riverside along Hunhe River and the wastewater drainage of Xihe River, and the extremely high values in the near suburb of the city's residential area were a striking feature, indicating the key role of city pollution in the spatial heterogeneity of soil DTPA-extractable zinc. When recorded in the form of a soil pollution map,the results of such a survey make it possible to identify the unusually polluted areas, and to provide more information for precise agriculture and environmental control.

  2. Alveolar epithelial permeability in patients with primary spontaneous pneumothorax as determined by Tc-99m DTPA aerosol scintigraphy

    International Nuclear Information System (INIS)

    Primary spontaneous pneumothorax (PSP) occurs subsequent to a disruption in the continuity of visceral pleura and escape of air into the pleural space. The cause of PSP is most often the rupture of subpleural blebs or bullae. It is usually difficult to detect evidence of pulmonary pathology. The purposes of the present study were to investigate the changes of pulmonary alveolar epithelial permeability in patients with PSP as determined by Tc-99m DTPA aerosol lung scintigraphy, to assess whether or not some differences exist between apical and basal parts of the lungs, and to determine the relationship between the clearance rate of Tc-99m DTPA and the pulmonary function test (PFT) results, the recurrence rate of PSP, and the percentage of pneumothorax in affected lung. Thirteen PSP patients (two females, 11 males; mean age 32.5±11.8 years) with normal chest X-ray were studied. Thirteen healthy non-smoking volunteers (1 female, 12 males; mean age, 35.8±10 years) were selected as a control group. Tc-99m DTPA aerosol lung scintigraphy and PFT were performed in all patients and controls. Clearance rates (%/min) of Tc-99m DTPA aerosol in right and left lung field, and apical and basal parts of each lung were calculated from dynamic images for 15 min. There was no significant difference (p>0.05) between patients and controls, or between apical and basal parts of each lung. No correlation was found between the clearance rate of Tc-99m DTPA and PFT results, the recurrence rate of PSP, or the percentage of pneumothorax. This study demonstrates that pulmonary epithelial permeability is not altered in PSP patients; the clearance rate of Tc-99m DTPA shows no difference between apical and basal parts of each lung. (author)

  3. Detection of alveolar epithelial injury by Tc-99m DTPA radioaerosol inhalation lung scan in rheumatoid arthritis patients

    International Nuclear Information System (INIS)

    Rheumatoid arthritis (RA) is a systemic autoimmune disorder primarily involving the joints. Lung alterations in RA may be primary or secondary to pharmacological treatments and may involve the alveoli, interstitium, airways and/or pleura. Technetium-99 m diethylenetriaminepentaacetic acid (Tc-99m DTPA) aerosol inhalation scintigraphy is a sensitive and noninvasive test commonly employed to assess pulmonary epithelial membrane permeability. The purpose of this study was to investigate the changes of pulmonary alveolar epithelial permeability in patients with RA, to determine the relationship between the clearance rate of Tc-99m DTPA and pulmonary function test (PFT) results, and to determine the relationship between the clearance rate of Tc-99m DTPA and clinical parameters of disease. Twenty-five patients with RA but without lung alterations were included in the study. The patients were 22 females, and 3 males; mean age 53.6±8.7 years. Technetium-99m DTPA aerosol inhalation scintigraphy was performed on the study and healthy control groups. Clearance half times (T1/2) were calculated by placing a mono-exponential fit on the curves. Penetration index (PI) was calculated on the first-minute image. There were no significant differences in the mean T1/2 or mean PI values between the RA patients and control subjects. No correlation was found between the mean T1/2 values of Tc-99m DTPA clearance and activity of RA, clinical values, or the spirometric measurements except FEV1/FVC and functional status in RA patients (p=0.02, p=0.01, respectively). However, a weak correlation was found between duration of disease and T1/2 values of Tc-99m DTPA clearance (p=0.006). PI values tended to correlate with FEF25-75, although, this was not statistically significant (p=0.057). This study shows that no changes occur in alveolar-capillary permeability in RA patients without lung alterations. (author)

  4. Preparation of 166 Dy/166 Ho DTPA-bis biotin as a system of In vivo generator

    International Nuclear Information System (INIS)

    The objective of this work was to synthesize the complex 166 Dy/166 Ho - diethylen triamine pentaacetic-bis Biotin (166 Dy/166 Ho DTPA-bis Biotin) to evaluate its potential as a new radiopharmaceutical in directed radiotherapy. The Dysprosium-166 was obtained for neutron irradiation of 164 Dy203 in the TRIGA Mark III reactor. The labelled was carried out in aqueous solution to p H 8.0 for addition of 166 Dy Cl3 to the diethylen triamine pentaacetic-α, ω-bis Biotin (DTPA-bis Biotin). The radiochemical purity was determined for HPLC and ITLC. The biological integrity of the marked biotin is evaluated by the biological recognition of the avidin for HPLC - molecular exclusion with and without avidin addition. The studies of stability in vitro were made in dilutions of saline solution to 0.9% and with human serum at 37 C incubated 1 and 24 hours. The complex 166 Dy/166 Ho DTPA-bis Biotin was obtained with a radiochemical purity of 99.1 ± 0.6%. The biological recognition of the complex 166 Dy/166 Ho DTPA-bis Biotin for the avidin it doesn't affect the labelling procedure. The studies in vitro demonstrated that the 166 Dy/166 Ho DTPA-bis Biotin is stable after the dilution in saline solution and in human serum that there is not translocation of the one radionuclide subsequent son to the beta decay of the 166 Dy that could produce the 166 Ho3+ liberation. The studies of Biodistribution in healthy mice demonstrated that the one complex 166 Dy/166 Ho DTPA-bis Biotin have a high renal distribution. In conclusion the radiolabelled biotin in this investigation has the appropriate properties to be used as an In vivo generator system stable for directed radiotherapy. (Author)

  5. Evaluation of renal allograft dysfunction using {sup 99m}Tc-DTPA dynamic SPECT. Analysis with two compartment model and graph plot

    Energy Technology Data Exchange (ETDEWEB)

    Akahira, Hideaki; Takekawa, Shoichi; Nigawara, Kazuo; Funyu, Tomihisa [Oyokyo Kidney Research Inst., Hirosaki, Aomori (Japan). Hirosaki Hospital

    1996-12-01

    To estimate renal blood flow and glomerular function in transplanted kidneys, we applied the 2 compartment model and the graphic analysis method to {sup 99m}Tc-DTPA dynamic SPECT and calculated some parameters, i.e. K1 (renal influx rate constant), K3 (glomerular filtration rate constant), Vdl (function phase distribution volume), and others. Twenty-three renal transplant recipients were examined and divided into following 3 groups according to their serum creatinine levels (SCr); Group I; less than 1.3 mg/dl (1.1{+-}0.3, n=7), Group II: 1.4-2.5 mg/dl (1.8{+-}0.3, n=7), and Group III more than 2.6 mg/dl (4.8{+-}2.9, n=5). The K3 value became lower in the order of Group I>II>III, and well correlated with blood urea nitrogen (BUN, r=<0.91, P<0.001) and creatinine clearance (Ccr, r=0.87, P<0.001). The K1 value reduced markedly in Group III despite or no difference between Group I and II. Glomerular filtration rate (GFR) derived from K1 and K3 showed a correlation with those by Tauxe`s method. From these results and clinical conditions including histopathological findings, it is suggested that K1, K3 and Vdl are useful paraments of renal central arterial blood flow, renal peripheral arteriolar blood flow and renal {sup 99m}Tc-DTPA uptake function, respectively. (author)

  6. Quantitation of blood-brain barrier defect by magnetic resonance imaging and gadolinium-DTPA in patients with multiple sclerosis and brain tumors

    DEFF Research Database (Denmark)

    Larsson, H B; Stubgaard, M; Frederiksen, J L;

    1990-01-01

    In this study quantitation of the degree of deficiency of the blood-brain barrier (BBB) in patients with multiple sclerosis or brain tumors, by using MRI, is shown to be possible. As a measure of permeability of the BBB to Gadolinium-DTPA (Gd-DTPA) the flux per unit of distribution volume per uni...

  7. Quantitation of blood-brain barrier defect by magnetic resonance imaging and gadolinium-DTPA in patients with multiple sclerosis and brain tumors

    DEFF Research Database (Denmark)

    Larsson, H B; Stubgaard, M; Frederiksen, Jette Lautrup Battistini;

    1990-01-01

    In this study quantitation of the degree of deficiency of the blood-brain barrier (BBB) in patients with multiple sclerosis or brain tumors, by using MRI, is shown to be possible. As a measure of permeability of the BBB to Gadolinium-DTPA (Gd-DTPA) the flux per unit of distribution volume per unit...

  8. Noncommutative Yang-Mills-Higgs actions from derivation-based differential calculus

    CERN Document Server

    Cagnache, Eric; Wallet, Jean-Christophe

    2008-01-01

    Derivations of a (noncommutative) algebra can be used to construct various consistent differential calculi, the so-called derivation-based differential calculi. We apply this framework to the noncommutative Moyal algebras for which all the derivations are inner and analyse in detail the case where the derivation algebras generating the differential calculus are related to area preserving diffeomorphisms. The ordinary derivations corresponding to spatial dimensions are supplemented by additional derivations necessarely related to additional covariant coordinates. It is shown that these latter have a natural interpretation as Higgs fields when involved in gauge invariant actions built from the noncommutative curvature. The UV/IR mixing problem for (some of) the resulting Yang-Mills-Higgs models is discussed. A comparition to other noncommutative geometries already considered in the litterature is given.

  9. Deriving Derivatives

    OpenAIRE

    Soklakov, Andrei N.

    2013-01-01

    Quantitative Structuring is a rigorous framework for the design of financial products. We show how it incorporates traditional investment ideas while supporting a more accurate expression of clients' views on the market. We briefly touch upon adjacent topics regarding the safety of financial derivatives and the role of pricing models in product design.

  10. The role of derivational paradigms with adjectival base in Old English word-formation

    Directory of Open Access Journals (Sweden)

    Carmen Novo Urraca

    2014-05-01

    Full Text Available The aim of this article is to identify the primary adjectives of Old English as well as to gather the derivational paradigms that revolve around them. All in all, 459 primary adjectives are identified, which function as base of 6,587 derivatives. Two conclusions are drawn from the analysis. In the first place, the concept of derivational paradigm contributes to the explanation of the overall organization of the lexicon, while allowing for the discussion of questions that are at the core of current morphological theory, such as recursivity and productivity. The conclusion is drawn that primary adjectives play a significant role in Old English derivation. Even though they are not as productive as strong verbs, primary adjectives function as base of derivation of a significant number of non-basic terms, which, moreover, belong to all lexical categories and nearly all grammatical classes.

  11. 99Tcm标记Gd-DTPA-DG及其在裸鼠体内的生物分布%Radio-labeling of Gd-DTPA-DG with 99Tcm and its biodistribution in nude mice

    Institute of Scientific and Technical Information of China (English)

    黄占文; 张伟; 陈跃

    2011-01-01

    Objective To observe the stability and biodistribution of 99 Tcm radio-labeled a paramagnetic deoxy-glucose-type MR contrast agent diethylenetriamine pentaacetic acid-gadolinium salt deoxy-glucose(Gd-DTPA-DG) in tumor-bearing nude mice in vivo. Methods To form 99 Tcm-Gd-DTPA-DG , the labelling conditions such as Gd-DTPA-DG quality, SnCl2 · 2 H2 O dosage , the reaction medium pH value and reaction temperature were optimized by orthogonal experimental design. The radio-labeling efficiency was measured by thin layer chromatography(TLC). The bio-distribution was observed by the tumor and other major organs were taken out from nude mice at 10,30 min and 1, 2,4 , 24 h respectively after caudal vein injection of 99 Tcm-Gd-DTPA-DG. Results When taking 10 mg Gd-DTPA-DG,0. 6 mg SnCl2 · 2H2O,pH<2,adding 37 MBq Na99 TcmO4 ,then reacting in boiling water for 30 min, the radiochemical purity of 99Tcm-Gd-DTPA-DG can reach to 98. 5% and remain 96. 2% placed at room temperature for 6 h. 99TcmGd-DTPA-DG accumulated in the tumor to a higher level than other organ at 2 h,the uptake was about (1. 48±0. 12) %ID/g , and the radio-uptake ratio of tumor to muscle(T/NT)was reached 2. 91. Conclusion 99 Tcm labeled the paramagnetic MR contrast agent Gd-DTPA-DG is feasible. The labeled agent shows excellent tumor targeting in nude mice in vivo. 99 Tcm-Gd-DTPA-DG is potential for a SPECT(single photon emission computed tomography)-MRI dual-modality imaging probes.%目的 探讨锝(99Tcm)标记顺磁性脱氧葡萄糖类MRI对比剂二乙三胺五乙酸-脱氧葡萄糖钆盐(Gd-DTPA-DG)的稳定性及在荷瘤裸鼠体内的生物分布,寻找一种核素与磁共振双模式影像探针.方法 对 Gd-DTPA-DG进行99Tcm标记,并对需要的Gd-DTPA-DG、氯化亚锡(SnCl2·2H2O)用量,pH,温度采用正交实验设计,确定最佳标记条件.采用薄层纸层析法(TLC)分析标记率,体外放置6 h,观察标记物的稳定性.将标记的99Tcm -Gd-DTPA-DG注入裸鼠体内,10、30

  12. A simple and inexpensive system for controlling body temperature in small animal experiments using MRI and the effect of body temperature on the hepatic kinetics of Gd-EOB-DTPA.

    Science.gov (United States)

    Murase, Kenya; Assanai, Purapan; Takata, Hiroshige; Saito, Shigeyoshi; Nishiura, Motoko

    2013-12-01

    The purpose of this study was to develop a simple and inexpensive system for controlling body temperature in small animal experiments using magnetic resonance imaging (MRI) and to investigate the effect of body temperature on the kinetic behavior of gadolinium ethoxybenzyl diethylenetriamine pentaacetic acid (Gd-EOB-DTPA) in the liver. In our temperature-control system, body temperature was controlled using a feedback-regulated heated or cooled air flow generated by two Futon dryers. The switches of the two Futon dryers were controlled using a digital temperature controller, in which the rectal temperature of a mouse measured by an optical fiber thermometer was used as the input. In experimental studies, male ICR mice aged 8weeks old were used and allocated into 5 groups (39-, 36-, 33-, 30-, and 27-degree groups, n=10), in which the body temperature was maintained at 39 °C, 36 °C, 33 °C, 30 °C, and 27 °C, respectively, using our system. The dynamic contrast-enhanced MRI (DCE-MRI) data were acquired with an MRI system for animal experiments equipped with a 1.5-Tesla permanent magnet, for approximately 43min, after the injection of Gd-EOB-DTPA into the tail vein. After correction of the image shift due to the temperature-dependent drift of the Larmor frequency using the gradient-based image registration method with robust estimation of displacement parameters, the kinetic behavior of Gd-EOB-DTPA was analyzed using an empirical mathematical model. With the use of this approach, the upper limit of the relative enhancement (A), the rates of contrast uptake (α) and washout (β), the parameter related to the slope of early uptake (q), the area under the curve (AUC), the maximum relative enhancement (REmax), the time to REmax (Tmax), and the elimination half-life of the contrast agent (T1/2) were calculated. The body temperature of mice could be controlled well by use of our system. Although there were no significant differences in α, AUC, and q among groups, there

  13. Utility of Gd-EOB-DTPA-Enhanced MRI in Diagnosing Small Hepatocellular Carcinoma

    Directory of Open Access Journals (Sweden)

    Soo Ryang Kim

    2009-07-01

    Full Text Available We describe an 8-mm hepatocellular carcinoma (HCC with hepatitis C virus-related cirrhosis in a 74-year-old woman. Ultrasound (US revealed an 8-mm hyperechoic nodule in segment 6 of the liver. Contrast-enhanced computed tomography (CT and US revealed no hypervascularity in the early phase and no washout in the late phase and the Kupffer phase, respectively. CT during arteriography revealed no hypervascularity and CT during arterial portography disclosed no perfusion defect. Gadolinium-ethoxybenzyl-diethylenetriamine pentaacetic acid (Gd-EOB-DTPA-enhanced magnetic resonance imaging (MRI revealed no hypervascularity in the early phase, but disclosed a defect in the hepatobiliary phase. Histologically, the nodule was diagnosed as well-differentiated HCC characterized by more than two-fold the cellularity of the non-tumorous area, with a high nuclear:cytoplasmic ratio, increased cytoplasmic eosinophilia, fatty change, and slight cell atypia with an irregular thin trabecular pattern. Our case demonstrates the utility of Gd-EOB-DTPA-enhanced MRI in the diagnosis of small HCC.

  14. Diffuse liver disease: MRI with Gd-DTPA versus CT and Ultrasound

    Energy Technology Data Exchange (ETDEWEB)

    Vogl, T.J. [Dept. of Radiology, Univ. of Munich (Germany); Steiner, S. [Dept. of Radiology, Univ. of Munich (Germany); Schnell, B. [Dept. of Radiology, Univ. of Munich (Germany); Gerbes, A. [Dept. of Internal Medicine 2, Univ. of Munich (Germany); McMahon, C. [Dept. of Radiology, Univ. of Munich (Germany); Wilimzig, C. [Dept. of Radiology, Univ. of Munich (Germany); Lissner, J. [Dept. of Radiology, Univ. of Munich (Germany)

    1992-08-01

    Twenty-nine patients with diffuse liver disease were examined by ultrasound, CT and MRI. MRI was performed using T1- and T2-weighted spin-echo sequences as well as fast gradient-echo-sequences. The paramagnetic contrast agent Gd-DTPA was applied intravenously (0.1 mmol/kg). In patients with hepatitis, MRI could be used in guiding liver biopsies as inflammatory changes were clearly delineated. CT and ultrasound were superior to MRI in the detection of focal or diffuse fatty degeneration. On the other hand MRI was more helpful in differentiating fatty changes and neoplasm. In liver cirrhosis, fibrotic changes were most clearly demonstrated by MRI. In patients suffering from hemochromatosis MRI offers advantages over CT and ultrasound in the diagnosis and follow up due to the paramagnetic properties of iron, resulting in a reduction in signal intensity. In patients with Wilson`s disease a characteristic pattern of parenchymal changes was seen. Administration of Gd-DTPA contributes additional information about perfusion conditions in the liver parenchyma, however this information was not of diagnostic relevance in the cases we studied. (orig.)

  15. Effects of sustained exercise on pulmonary clearance of aerosolized 99mTc-DTPA

    International Nuclear Information System (INIS)

    The effects of intensive prolonged exercise on the pulmonary clearance rate of aerosolized 99mTc-labeled diethylenetriaminepentaacetate (99mTc-DTPA) and pulmonary mechanics were studied in seven healthy nonsmoking volunteers. 99mTc-DTPA clearance and pulmonary mechanics (lung volumes and compliance) were assessed before and after 75 min of constant-load exercise performed on a treadmill, corresponding to 75% of maximal O2 uptake. Because both clearance measurements were made in similar conditions of pulmonary blood flow, respiratory rate, and tidal volume, changes in clearance rate can be assumed to represent changes of alveolar epithelial permeability. After exercise, total, apical, and basal clearance were significantly increased (P less than 0.01, 0.05, and 0.05, respectively) and the increases in total clearance and tidal volume observed during exercise were significantly correlated (P less than 0.05). In contrast, no significant change was found in pulmonary mechanics. These results show that prolonged intensive exercise induces an increase in epithelial permeability, which appears to be related to the mechanical effects of sustained increased ventilation. Because no change was evidenced in pulmonary volumes or in lung elasticity, our results suggest that this increase may result from alteration of the intercellular tight junctions rather than from a surfactant deficiency

  16. MRI of retroperitoneal lesions using a rapid pulse sequence method and Gd-DTPA enhancement

    Energy Technology Data Exchange (ETDEWEB)

    Yoshida, Hideo; Yashiro, Naobumi; Iio, Masahiro; Mano, Isamu.

    1988-04-01

    13 retroperitoneal lesions were examined using a rapid pulse sequence method of MRI, which provided an image in 26 to 51 seconds during respiratory holding of the patients. Pulse sequence utilized was spin echo with TR = 100 to 200 msec and TE = 30 or 40 msec. Dynamic studies of ten of 13 cases were examined using Gd-DTPA (Gadolinium-diethylenetriamine pentacetic acid) at 0.05 mmol/kg for each patient. We scanned each patient using coronal or sagittal mode without misresistration by holding respiration. The results were as follows: 1) Our method well demonstrated normal adrenal gland surrounded with highly contrast fat tissue and could differentiate cotex and medulla of normal kidney. 2) Hemorrhagic parts in the tumor or the cyst could be more visualized than by X-ray CT. 3) Our method could well visualize tumors containing a lot of adipose tissue as high signal and bone or bone marrow as low signal. 4) Morphologic and kinetic features of adrenal and renal tumors were clearly obtained using dynamic studies of Gd-DTPA enhancement.

  17. DTPA-Extractable Zinc in Rice Soils and its Availability to Rice

    Directory of Open Access Journals (Sweden)

    Dhaneshwar Padhan

    2016-08-01

    Full Text Available Zinc (Zn deficiency is a fairly wide spread agronomic constraint in many of the world rice production regions. Information on soil Zn distribution is essential for understanding its chemical reactions and bioavailability. In this backdrop, we tried to find out the relationship between physic-chemical properties and DTPA-extractable Zn (available Zn content of rice soils. For this eighty four (84 surface soil samples (0-20cm were collected from three villages (viz. Saharapali, Nuagarh and Adgaon of Bargarh district under the Hirakud Command Area of Odisha. Analytical observations revealed that the soils were slightly acidic in reaction with moderately high content of soil organic carbon. Considering the critical limit of available Zn in soils to be 0.6 mg kg-1, all soils of the study area were found to be well supplied with DTPA-extractable Zn. Soil organic carbon (SOC content maintained positive and significant correlation with available zinc content in soils. However, soil pH showed significant but negative correlation with available zinc content of soils.

  18. Scintiscanning with aerosols of 99Tcm DTPA in the diagnostic strategy for pneumocystosis in aids patients

    International Nuclear Information System (INIS)

    Pulmonary clearance of an aerosol of 99Tcm DTPA was measured in 21 non-smoking AIDS and pneumocystosis patients. The results were compared with those of pulmonary scintiscanning with gallium. All the patients exhibited increased clearance, evidence of a considerable alteration in alveolar permeability. This increase was also observed in patients with normal chest X-rays and normal blood gases. The average clearance in these patients was 6 ± 2% min-1 as compared with a normal 1.1 ± 0.3% min-1. Conversely, only four out of eight patients with normal chest X-rays had abnormal gallium scans. Clearance returned to normal after recovery. Measurement of DTPA clearance appears to be a very sensitive indicator for the detection and follow-up of pneumocystosis. However, it is not very specific since it can be increased by tobacco poisoning, drug abuse and the presence of lymphocytic alveolitis. An examination with normal findings can therefore exclude the possibility of opportunistic pneumopathy and make bronchial lavage unnecessary. An examination with abnormal findings should show whether lavage is indicated, even if the chest X-rays, blood gases and gallium scans are normal. (author). 9 refs, 2 tabs

  19. Synthesis and characterisation of [90Y]-Bz-DTPA-oct: a yttrium-90-labelled octreotide analogue for radiotherapy of somatostatin receptor-positive tumours

    International Nuclear Information System (INIS)

    An investigation into the in vitro behaviour of two yttrium-90-labelled somatostatin analogues was performed. Further in vivo characterisation was performed with the most promising agent. A new DTPA-octreotide analogue (Bz-DTPA-oct) was synthesised by coupling a bifunctional DTPA chelator to the N-terminal amine of the D-Phe1 of Tyr3-octreotide. This new SRIF analogue and DTPA-octreotide (OctreoScan) were radiolabelled with 90Y prior to serum stability being evaluated. Receptor binding assays were also performed on the two radioligands using rat cortex membranes. The [90Y]-Bz-DTPA-oct was further evaluated in vivo using tumour-bearing rats. The first conjugate (DTPA-octreotide) bound with a high affinity to SRIF receptors and the 90Y complex was relatively stable in human serum (t 1/2 3.8 d for 90Y lost to serum proteins). The second conjugate (Bz-DTPA-oct) also exhibited a high binding affinity to SRIF receptors, but it demonstrated an even slower loss of 90Y to serum proteins (t1/2 12.1 d). The in vivo evaluation of the more stable [90Y]-Bz-DTPA-oct showed a very rapid and high accumulation in somatostatin receptor-positive tumours, which after 1 h resulted in tumour/nontumour ratios of 3.8, 21, and 4.9 (for blood, muscle, and liver, respectively). These tumour/nontumour ratios increased, and were by 24 h postinjection 138, 285, and 6.1 (for blood, muscle, and liver). Yttrium-90-labelled Bz-DTPa-oct is rapidly and selectively accumulated in somatostatin receptor-positive tissue. Octadentate Bz-DTPA-oct could be ligand for 90Y radiotherapy of somatostatin receptor-positive tumours and their metastases

  20. Evaluation of three algorithms to calculate the relative renal function with {sup 99}Tc-DTPA; Evaluation de trois algorithmes pour calculer la fonction renale relative au {sup 99}Tc-DTPA

    Energy Technology Data Exchange (ETDEWEB)

    Charfeddine, S.; Maaloul, M.; Kallel, F.; Chtourou, K.; Guermazi, F. [EPS Habib Bourguiba, Service de Medecine Nucleaire, Sfax (Tunisia)

    2006-06-15

    The aim of our study is to estimate the reproducibility and the exactitude of three algorithms to determine with {sup 99m}Tc-DTPA the relative function of each kidney. Methods: a prospective study was carried out in voluntary patients. Reproducibility was studied in 11 patients who underwent had two examinations with {sup 99m}Tc-DTPA. Exactitude was evaluated in 35 patients who had an additional scintigraphy with {sup 99m}Tc-DMSA taken as a reference. To determine the relative renal function with {sup 99m}Tc-DTPA, three algorithms using various background subtraction methods and time intervals were applied. Results and conclusion: the method of the integral was the most reproducible and exact. It was little influenced by the choice of the interval of time. The reproducibility and the exactitude of the Patlak method were worse, especially in case of renal insufficiency or hydronephrosis. A high background and poor counting statistics explain why Patlak was less powerful with {sup 99m}Tc-DTPA than with {sup 99m}Tc-MAG3. The method of the slopes should not be recommended any more. (author)

  1. Effect of desferrioxamine (DFO) and calcium trinatrium diethylenetriaminepentaacetic acid (DTPA) on rat cytomegalovirus replication in vitro and in vivo.

    Science.gov (United States)

    Kloover, J S; Scholz, M; Cinatl, J; Lautenschlager, I; Grauls, G E; Bruggeman, C A

    1999-11-01

    Cytomegalovirus (CMV) infection is a major problem in the immunosuppressed patient. It is thought that besides direct CMV induced cell lysis, immunological damage is part of CMV pathogenesis. New antiviral drugs, which combine immunomodulating and antiviral qualities, could be beneficial. Recently, it has been described that desferrioxamine (DFO) and calcium trinatrium diethylenetriaminepentaacetic acid (DTPA) exhibit both properties. In this report the antiviral effects of both compounds against rat CMV (RCMV) are described in vitro and in vivo using a generalised and local infection model. In vitro, both compounds exhibited a significant antiviral effect, DTPA being more potent than DFO. However, in the generalised infection model no effect was seen on mortality, morbidity or presence of virus in internal organs. In rats infected subcutaneously in the hind paw, no effect was seen locally on paw thickness, presence of viral antigens and inflammatory response. In addition, these rats suffered from a generalised infection of low magnitude at 15 days post infection, although both DFO and DTPA were able to lower the level of viral replication. In conclusion, our data indicate that despite in vitro activity, in vivo usage of DFO or DTPA for acute CMV infection is not warranted.

  2. Biokinetics of 111In-DTPA-D-Phe1-octreotide in nude mice transplanted with a human carcinoid tumor

    International Nuclear Information System (INIS)

    The long time biokinetics of the radiolabeled somatostatin analogues 111In-DTPA-D-Phe1-octreotide was studied in nude mice transplanted with the human carcinoid tumor, GOT1. The results were compared with those from the patient with the original tumor. This patient has been diagnosed and later treated with 111In-DTPA-D-Phe1-octreotide. The animals received about 2 MBq 111In-DTPA-D-Phe1-octreotide (0.1 μg) by injection into a tail vein. The animals were killed 0.5 h-14 d after injection of the radiopharmaceutical. Tumor tissue and normal tissues were collected and weighed and measured for 111In activity. The 111In uptake in the tumor was higher than in all normal tissues except the kidneys. The tumor-to-normal-tissue activity concentration, TNC, increased with time for all normal tissues studied. These data were similar to those observed for the original tumor in the patient. The similar biokinetics for 111In-DTPA-D-Phe1-octreotide in the tumor-bearing mice and the patient makes this animal model suitable as a model for evaluation of therapy of somatostatin receptor (sstr) expressing tumors with radiolabeled somatostatin analogues. Furthermore, the increase with time of TNC both in mice and the patient indicates that long-lived radionuclides are preferred for therapy with radiolabeled somatostatin analogues

  3. Serial assessment of the area at risk in myocardial infarction with Gd-DTPA-enhanced MR imaging in humans

    International Nuclear Information System (INIS)

    Experimental studies have shown that the region of increased myocardial signal intensity with Gd-DTPA correlates with the area at risk but overestimates the infarct size. The authors have assessed the evolution of the area at risk in acute myocardial infarction in seven patients, using Gd-DTPA enhanced MR imaging at 1 and 2 weeks after the acute event. Multisection MR imaging of the total left ventricle was performed at 0.5 T after injection of 0.2 mmol/kg of Gd-DTPA. The area with a signal intensity greater than that of normal myocardium (± 2SDs) was designated as the area at risk in each section. The summation of these areas was measured by two observers at 1 and 2 weeks after infarction; intra- and interobserver variability was 3%. The area at risk ranged from 3% to 18%; at 2 weeks the size of the area at risk showed only slight changes (P = not significant). The authors discuss how Gd- DTPA enhances the area at risk and may be useful in assessing the evolution of the size of this area

  4. Cationic Gd-DTPA liposomes for highly efficient labeling of mesenchymal stem cells and cell tracking with MRI

    NARCIS (Netherlands)

    J. Guenoun (Jamal); G.A. Koning (Gerben); R.Q. Doeswijk (Ronald); L. Bosman (Lizanne); P.A. Wielopolski (Piotr); G.P. Krestin (Gabriel); M.R. Bernsen (Monique)

    2012-01-01

    textabstractIn the current study cell labeling was performed with water-soluble gadolinium (Gd)-DTPA containing liposomes, to allow for cell tracking by MRI. Liposomes were used to assure a highly concentrated intracellular build up of Gd, aiming to overcome the relatively low MRI sensitivity of Gd

  5. Capillary transfer constant of Gd-DTPA in the myocardium at rest and during vasodilation assessed by MRI

    DEFF Research Database (Denmark)

    Fritz-Hansen, T; Rostrup, Egill; Søndergaard, Lise;

    1998-01-01

    The purpose of this study was to determine whether the capillary transfer constant (Ki) of gadolinium-DTPA was sensitive to perfusion changes and whether ischemic regions in the myocardium could be identified using the modified Kety formula. Ki was measured at rest and during dipyridamole-induced...

  6. Measurement of the arterial concentration of Gd-DTPA using MRI: a step toward quantitative perfusion imaging

    DEFF Research Database (Denmark)

    Fritz-Hansen, T; Rostrup, Egill; Larsson, H B;

    1996-01-01

    A noninvasive method using an inversion recovery turbo-FLASH for dynamic measurement of the arterial input function represented by the bolus passage of Gd-DTPA in the descending aorta is presented, and the results are compared with the input function obtained by arterial blood samples. A good acc...

  7. Measurement of blood-brain barrier permeability using dynamic Gd-DTPA scanning--a comparison of methods

    DEFF Research Database (Denmark)

    Larsson, H B; Tofts, P S

    1992-01-01

    Two recently published methods of blood-brain barrier permeability measurement using Gd-DTPA scanning are compared by authors representing each group. The physiological models are reconciled. Results from both groups agree. These show that the transfer constant (the permeability surface area prod...

  8. In vivo quantification of the unidirectional influx constant for Gd-DTPA diffusion across the myocardial capillaries with MR imaging

    DEFF Research Database (Denmark)

    Larsson, H B; Stubgaard, M; Søndergaard, Lise;

    1994-01-01

    The authors present an in vivo method for measuring the unidirectional influx constant (Ki) for gadolinium diethylenetriaminepentaacetic acid (DTPA) diffusion across the capillary membrane in the human myocardium with magnetic resonance imaging. Ki is related to the extraction fraction (E) and th...

  9. Use of /sup 99m/Tc-DTPA for detection and localization of site of acute gastrointestinal bleeding

    Energy Technology Data Exchange (ETDEWEB)

    Abdel-Dayem, H.; Owuwanne, A.; Nawaz, K.; Kouris, K.; Higazy, E.; Mahajan, K.; Ericsson, S.; Awdeh, M.

    1988-05-01

    Intravenously injected /sup 99m/Tc-DTPA was evaluated in 64 patients for its efficiency in detecting and localizing sites of acute upper and lower gastrointestinal (G.I.) bleeding. These studies were correlated with endoscopic and surgical findings. There were 34 bleeders and 30 non bleeders giving a sensitivity of 90%, specificity of 82% and accuracy of 86%. Of these, 49 were upper G.I. studies (stomach 21 and duodenum 28) and 15 were lower G.I. studies (small intestine 8, large bowel 7). Of the 49 upper G.I. studies, 27 showed active bleeding while 22 showed no bleeding at the time of the study resulting in a sensitivity of 87.5%, specificity of 76% and accuracy of 82%. Of the 15 lower G.I. studies, 7 were bleeders while 8 were non bleeders. All the lower G.I. bleeding sites were accurately localized with the /sup 99m/Tc-DTPA. An incidental finding of these studies was the localization of /sup 99m/Tc-DTPA in the site of inflammatory and malignant lesions of the G.I. tract. Of the 64 studies, 18 inflammatory and malignant lesions were detected with the IV injected /sup 99m/Tc-DTPA; 10 were bleeders while 8 were non bleeders. Image subtraction of early from delayed images was helpful to differentiate bleeding from non bleeding cases in this last group of studies.

  10. The use of 99mTc-DTPA for detection and localization of site of acute gastrointestinal bleeding

    International Nuclear Information System (INIS)

    Intravenously injected 99mTc-DTPA was evaluated in 64 patients for its efficiency in detecting and localizing sites of acute upper and lower gastrointestinal (G.I.) bleeding. These studies were correlated with endoscopic and surgical findings. There were 34 bleeders and 30 non bleeders giving a sensitivity of 90%, specificity of 82% and accuracy of 86%. Of these, 49 were upper G.I. studies (stomach 21 and duodenum 28) and 15 were lower G.I. studies (small intestine 8, large bowel 7). Of the 49 upper G.I. studies, 27 showed active bleeding while 22 showed no bleeding at the time of the study resulting in a sensitivity of 87.5%, specificity of 76% and accuracy of 82%. Of the 15 lower G.I. studies, 7 were bleeders while 8 were non bleeders. All the lower G.I. bleeding sites were accurately localized with the 99mTc-DTPA. An incidental finding of these studies was the localization of 99mTc-DTPA in the site of inflammatory and malignant lesions of the G.I. tract. Of the 64 studies, 18 inflammatory and malignant lesions were detected with the IV injected 99mTc-DTPA; 10 were bleeders while 8 were non bleeders. Image subtraction of early from delayed images was helpful to differentiate bleeding from non bleeding cases in this last group of studies. (orig.)

  11. Preparation of 177Lu-DOTA/DTPA-Bz-Cys-RGD dimer and biodistribution evaluation in normal mice

    International Nuclear Information System (INIS)

    177Lu-DOTA-Bz-Cys-RGD dimer and 177Lu-DTPA-Bz-Cys-RGD dimer were prepared, and the in vitro and in vivo properties were compared. TLC and HPLC show that the labeling yields of two radiolabeled compounds are more than 95% under optimal conditions (pH=5.0, reacting at 100 degree C for 15-20 min), and the two radiolabeled compounds show pretty good in vitro stability. HPLC analyses and lg P values reveal that lipophilicity of 177Lu-DOTA-Bz-Cys- RGD dimer is higher than 177Lu-DTPA-Bz-Cys-RGD dimer. The uptake of 177Lu-DTPA-Bz-Cys- RGD dimer in other tissues is significantly higher than that of 177Lu-DOTA-Bz-Cys-RGD dimer at 4 h postinjection, except for blood and spleen. The in vivo stability of 177Lu-DOTA-Bz-Cys-RGD dimer is much better than 177Lu-DTPA-Bz-Cys-RGD dimer. Bz-DOTA is an ideal bifunctional chelator for 177Lu labeling of RGD dimer. (authors)

  12. Determination of kidney function with 99mTc-DTPA renography using a dual-head camera

    DEFF Research Database (Denmark)

    Madsen, Claus J; Møller, Michael L; Zerahn, Bo;

    2013-01-01

    Single-head gamma camera renography has been used for decades to estimate kidney function. An estimate of the glomerular filtration rate (GFR) can be obtained using Tc-diethylenetriaminepentaacetic acid (Tc-DTPA). However, because of differing attenuation, an error is introduced when the kidney...

  13. Clinical studies of alveolar-capillary permeability using technetium-99m DTPA aerosol

    International Nuclear Information System (INIS)

    Soluble radioaerosols such as technetium-99m diethylene triamine pentacetate (DTPA) permit simple quantitative studies of alveolar-capillary permeability to be performed, since the submicronic aerosols are deposited mainly at the lung periphery and are cleared across the alveolar-capillary membrane. Regional alterations in permeability can also be noted using this radionuclide technique. We have measured the pulmonary epithelial permeability in normal subjects and the alteration in smokers, glue-sniffers, patients with inhalation burns, chronic obstructive pulmonary disease (COPD) and patients with lung metastases from thyroid cancer treated with radioiodine 131I. In the normal volunteers, the time taken for 50% of inhaled 99mTc DTPA to be cleared from the lungs (T1/2) was 66 minutes±1 sd of 12 mins. The smokers had a mean T1/2 of 20 mins±1 sd 4 min. In the hard-core glue-sniffing group, the majority were smokers who had stopped smoking and glue-sniffing for periods varying from 1 day to 42 days, and it was possible to note the changes in clearance times against period of abstinence. In the patients with inhalation burns, there was change in lung clearance arising from pulmonary epithelial damage; these patients showed increased rate of clearance (short T1/2) with mean T1/2 of 36 min±1 sd of 11 mins, while the retention images revealed regional lung damage in moderately severe inhalation burns. Twenty-four patients with COPD had inhalation scans done with Tc-99m tin colloid radioaerosol, and these images were compared with the perfusion lung scans done with 99mTc macroaggregated albumin (MAA); in general the perfusion images matched the defects noted in the inhalation scans. The 99mTc DTPA clearance rate in these patients was normal i.e. T1/2=78±14 mins. In the thyroid cancer patients with lung metastases, who had high doses of radioiodine treatment, the T1/2 values were normal or prolonged slightly, mean T1/2=76 min±23. (author)

  14. Development and Characterization of Novel Films Based on Sulfonamide-Chitosan Derivatives for Potential Wound Dressing

    OpenAIRE

    Oana Maria Dragostin; Sangram Keshari Samal; Florentina Lupascu; Andreea Pânzariu; Peter Dubruel; Dan Lupascu; Cristina Tuchilus; Cornelia Vasile; Lenuta Profire

    2015-01-01

    The objective of this study was to develop new films based on chitosan functionalized with sulfonamide drugs (sulfametoxydiazine, sulfadiazine, sulfadimetho-xine, sulfamethoxazol, sulfamerazine, sulfizoxazol) in order to enhance the biological effects of chitosan. The morphology and physical properties of functionalized chitosan films as well the antioxidant effects of sulfonamide-chitosan derivatives were investigated. The chitosan-derivative films showed a rough surface and hydrophilic prop...

  15. Semi-automatic detection of Gd-DTPA-saline filled capsules for colonic transit time assessment in MRI

    Science.gov (United States)

    Harrer, Christian; Kirchhoff, Sonja; Keil, Andreas; Kirchhoff, Chlodwig; Mussack, Thomas; Lienemann, Andreas; Reiser, Maximilian; Navab, Nassir

    2008-03-01

    Functional gastrointestinal disorders result in a significant number of consultations in primary care facilities. Chronic constipation and diarrhea are regarded as two of the most common diseases affecting between 2% and 27% of the population in western countries 1-3. Defecatory disorders are most commonly due to dysfunction of the pelvic floor or the anal sphincter. Although an exact differentiation of these pathologies is essential for adequate therapy, diagnosis is still only based on a clinical evaluation1. Regarding quantification of constipation only the ingestion of radio-opaque markers or radioactive isotopes and the consecutive assessment of colonic transit time using X-ray or scintigraphy, respectively, has been feasible in clinical settings 4-8. However, these approaches have several drawbacks such as involving rather inconvenient, time consuming examinations and exposing the patient to ionizing radiation. Therefore, conventional assessment of colonic transit time has not been widely used. Most recently a new technique for the assessment of colonic transit time using MRI and MR-contrast media filled capsules has been introduced 9. However, due to numerous examination dates per patient and corresponding datasets with many images, the evaluation of the image data is relatively time-consuming. The aim of our study was to develop a computer tool to facilitate the detection of the capsules in MRI datasets and thus to shorten the evaluation time. We present a semi-automatic tool which provides an intensity, size 10, and shape-based 11,12 detection of ingested Gd-DTPA-saline filled capsules. After an automatic pre-classification, radiologists may easily correct the results using the application-specific user interface, therefore decreasing the evaluation time significantly.

  16. The measurement of 99mTc-DTPA pulmonary clearance in normals, asymptomatic smokers and diabetic patients

    International Nuclear Information System (INIS)

    We measured pulmonary epithelial permeability by 99mTc-DTPA radioaerosol clearance in patients with diabetes and correlated with the presence of microangiopathy to understand the pathophysiology of pulmonary microangiopathy and evaluate 99mTc-DTPA radioaerosol clearance as a diagnostic test to assess pulmonary microangiopathy. We performed 99mTc-DTPA radioaerosol scan in 10 normal subjects, 10 asymptomatic smokers, 20 diabetic patients without history of smoking (10 with microangiopathy, 10 without microangiopathy). 99mTc-DTPA clearance half-time (T1/2) was calculated, then compared with the result of chest radiography and pulmonary function test. Chest radiography and pulmonary function test were normal in all subjects. There were no significant difference of clinical or laboratory characteristics between these groups except age. The diabetic patients with microangiophaty were significantly older (p1/2 of normal subjects and asymptomatic smokers were significantly different (65.2±23.7 min vs 39.6±9.8 min, p1/2 was 90.5±46.5 min and significantly delayed when compared with those of normals and asymptomatic smokers (p1/2 of diabetic patients without microangiopathy, 70.0±12.7 min, was not significantly different from those of normals or asymptomatic smokers (p>0.05). No significant correlation was found between the T1/2 and spirometric parameters including DLco, FVC, FEV1, FEV1/FVC (%) and FEF25-75% in all subjects, and between the T1/2 and duration of diabetes in diabetic patients. Eventhough the influence of age can't be excluded, delayed 99mTc-DTPA clearance half-time (T1/2) in diabetic patients with microangiopathy indicates decreased pulmonary capillary permeability as one of the pathophysiologic results of pulmonary microangiopaththy. Further studies are needed in larger number of age matched control and diabetic patients to evaluate the diagnostic efficacy

  17. Effect of early and delayed DTPA therapy on retention of plutonium citrate in mouse and dog testis

    International Nuclear Information System (INIS)

    Studies by Green et al. (Nature 255, 77, 1975) have indicated preferential irradiation of spermatogonial stem cells, with increased potential for genetic risk, as a result of nonhomogeneous deposition of plutonium in the mouse testis. Further genetic studies in this laboratory raised the question whether, as in other tissues, decorporation therapy with diethylenetriaminepentaacetic acid (DTPA) would reduce the testicular Pu burden and, consequently, the genetic risk. Accordingly, 27 B6CF1 male mice were intravenously injected with 10 μCi/kg of monomeric 239Pucitrate. Saline-treated controls were sacrificed 6 hrs, 6 days and 100 days later. Two groups of 5 mice each were treated intraperitoneally with a maximally effective dose of DTPA (100 mg/kg, as the calcium chelate) twice per week for 12 weeks, beginning either at 6 hrs or at 6 days. At necropsy, 100 days after Pu injection, testes were removed for gross Pu analysis and quantitative autoradiography. The same two treatment regimens (except injection was intravenous) were given to 15 ANL beagle dogs that had received an intravenous injection of 0.3 μCi/kg of monomeric 239Pucitrate. Saline-treated controls, in groups of 3, were sacrificed at 6 hrs, 6 days and 90 days. In both species, DTPA treatment begun earlier was more effective in reducing the testicular burden. After 12 weeks of treatment, DTPA begun at 6 hours resulted in an 8- to 10-fold reduction as compared to controls, whereas DTPA treatments begun at 6 days resulted in a 4- to 5-fold reduction. Autoradiographic track counts indicated approximately equal removal of Pu from all deposition areas of the testis. (author)

  18. Decorporation of plutonium by pulmonary administration of Ca-DTPA dry powder: a study in rat after lung contamination with different plutonium forms

    Energy Technology Data Exchange (ETDEWEB)

    Serandour, A.L.; Grillon, G.; Frechou, M.; Fritsch, P.; Poncy, J.L. [CEA/DSV/DRR, Laboratoire de Radiotoxicologie, BP 12, 91680 Bruyeres-Le-Chatel (France); Tsapis, N.; Gervelas, C.; Fattal, E. [Universite Paris-Sud 11 CNRS, UMR 8612, Physico-chimie-Pharmacotechnie-Biopharmacie, 92296 Chatenay-Malabry (France); Deverre, J.R. [CEA/DSV/DRM, Service Hospitalier Frederic Joliot, 91401 Orsay (France); Benech, H. [CEA/DSV/DRM, Service de Pharmacologie et d' Immunologie, 91191, Gif-sur-Yvette (France)

    2007-07-01

    This study evaluates the decorporation efficacy of a pulmonary administration of a new Ca-DTPA (diethylene-triamine-pentaacetic acid) dry powder (18 {mu}mol kg{sup -1} of body mass) after pulmonary contamination of rats with different Pu compounds. After inhalation of PuO{sub 2}, a delayed intratracheal administration of DTPA cannot reduce significantly the retention of Pu in the lungs but limits its transfer in liver and skeleton. After pulmonary contamination by Pu nitrate, early insufflation of the DTPA powder appears twice as more efficient than an i.v injection of DTPA (30 {mu}mol kg{sup -1}) to reduce Pu retention in the lungs and is as effective as i.v. injection to limit the extra-pulmonary deposit. In contrast, a delayed administration of DTPA cannot reduce the lung or extra-pulmonary retention. In conclusion, the improvement of aerodynamic properties of DTPA powder leads to an increase of DTPA amount deposited in the lungs and enhances the body decorporation. (authors)

  19. Decorporation of plutonium by pulmonary administration of Ca-DTPA dry powder: a study in rat after lung contamination with different plutonium forms

    International Nuclear Information System (INIS)

    This study evaluates the decorporation efficacy of a pulmonary administration of a new Ca-DTPA (diethylene-triamine-pentaacetic acid) dry powder (18 μmol kg-1 of body mass) after pulmonary contamination of rats with different Pu compounds. After inhalation of PuO2, a delayed intratracheal administration of DTPA cannot reduce significantly the retention of Pu in the lungs but limits its transfer in liver and skeleton. After pulmonary contamination by Pu nitrate, early insufflation of the DTPA powder appears twice as more efficient than an i.v injection of DTPA (30 μmol kg-1) to reduce Pu retention in the lungs and is as effective as i.v. injection to limit the extra-pulmonary deposit. In contrast, a delayed administration of DTPA cannot reduce the lung or extra-pulmonary retention. In conclusion, the improvement of aerodynamic properties of DTPA powder leads to an increase of DTPA amount deposited in the lungs and enhances the body decorporation. (authors)

  20. Use of activable cations as tracers in groundwater hydrology. The case of DTPA-Indium

    International Nuclear Information System (INIS)

    The possibilities of EDTA, CDTA and DTPA metallic complexes use as activable groundwater, tracers are discussed. Indium, which has good nuclear caracteristics for activation analysis and forms complexes of great stability with polyamino carboxylic acid has been for Laboratory and field studies. For corporative studies, Rhodomine B, a fluorescent tracer have been studied together with Indium complexes. In laboratory retention studies have been carried with In-EDTA, Iodine 131 and Rhodomine B, as tracers and bentonite, zeolite 13X and Dowex-1 and Dowex-50 as sorbents. As field studies, drainage evolution flow and resident time distribution of tracers substances in water, have been carried, under artificial rain conditions realized by aspersion. Results from field studies showed good characteristics of Indium Complexes especially in very absorbent medium (argilaceous limon) where their restitution balance were superior to that of Rhodomine B

  1. Brain MR in patients with Langerhans cell histiocytosis: Findings and enhancement with Gd-DTPA

    International Nuclear Information System (INIS)

    The brains of eight patients with Langerhans Cell Histiocytosis (LCH) were studied with magnetic Resonance Imaging (MRI). One scan was normal and a spectrum of abnormalities was seen in the others. Five patients had absence of the posterior pituitary bright spot, and four with evidence of pituitary dysfunction had a lesion in the region of the hypothalamus. An orbital extraconal mass was noted in one child with exopthalmus. This decreased in a follow-up study after Vinblastine therapy. The cerebellum was abnormal in two patients; the area of the dentate nuclei exhibited abnormal signal in one asymptomatic child and the MRI of another boy with a ten year history of progressive cerebellar dysfunction showed cerebral and cerebellar atrophy. Three of the five patients with deficiency of antidiuretic hormone (ADH) were studied both before and after the administration of intravenous gadolinium DTPA. In all three, after administration of the contrast agent, there was enhancement of involved areas. (orig.)

  2. Imaging fetal renal function by means of a fast MR imaging sequence with Gd-DTPA

    International Nuclear Information System (INIS)

    Although the use of magnetic resonance (MR) imaging in obstetrics has been reported, this modality has not yet been shown to offer more or different information than ultrasound. The authors studied fetal renal function by injecting Gd-DTPA (100 μg/kg) into a fetal lamb indwelling femoral vein catheter at the start of a fast low-angle shot (FLASH) sequence (repetition time, 06 sec; echo time, 13 msec; flip angle, 400 or 200, one acquisition). Identically positioned images were generated every 8 seconds for 5 minutes and less frequently thereafter. Newborn and older lambs and some adult sheep were similarly studied for comparison. Time-intensity curves from several distinct regions of interest in the kidney demonstrate maturation-related changes in renal handling of the contrast agent

  3. The feasibility of a renogram study in dogs with radiopharmaceutical technetium 99m-DTPA

    International Nuclear Information System (INIS)

    The present study is one of in vivo technetium 99m-DTPA renography, successively involving conscious healthy dogs under acepromazine maleate sedation and dogs under the narcotic sodium thiopental. It was found that during the running of the renogram the animal had to be kept completely immobile and that constant infusion narcosis with sodium thiopental produced this immobility without affecting the renograms unduly. However administration of a thiopental bolus did have an adverse effect on the renogram. Sedation with acepromazine maleate significantly increased the time to peak and the excretion phase as presented by the slope. These effects are thought to be due to a decreased blood pressure with concomitant renal bloodpooling and retarded bloodflow. The radioisotope renogram appears to hold great promise for both clinical and research applications. The equipment required for this application however, is so costly that it would only be financially feasible for major centres

  4. C60-Based Ebselen Derivative: Synthesis and Enhanced Protective Effect on Mouse Thymus Cells

    Institute of Scientific and Technical Information of China (English)

    LIU,Xu-Feng; GUAN,Wen-Chao; KE,Wen-Shan

    2008-01-01

    A C60-based ebselen derivative 3 was synthesized through a Bingel cyclopropanation of C60 with the ebselen malonate 2. It was obtained in a three-step synthesis starting from 2-(chloroseleno)benzoyl chloride and 2-(2-aminoethoxy)ethanol, in a 42% yield (based on consumed C60). Its structure was characterized by 1H NMR,13C NMR, IR, FAB-MS, and elemental analyses techniques. To verify that the C60-based ebselen derivative 3 had enhanced effect on viability of mouse thymus cells, the C60 derivative 4 and ebselen derivative 2 were selected to treat the mouse thymus cells using the same procedures as those with the C60-based ebselen derivative 3. The result shows that MTT(OD) values of compound 3 treated groups (0.335 ±0.021) were all higher than those of compound 4 (0.283±0.031) and compound 2 (0.247±0.025) treated groups, indicating that the compound 3 has an advantage over compounds 2 and 4 in promoting the viability of the mouse thymus cell.

  5. Assessment of pulmonary aerosol deposition and epithelial permeability in 99mTc-DTPA inhalation scintigram

    International Nuclear Information System (INIS)

    The degree of lung injury in bronchiolo-alveolar lesions may be quantitated from the pulmonary epithelial permeability estimated by 99mTc-diethylene triamine penta acetate (DTPA) aerosol inhalation scintigram. However, significant aerosol deposition sometimes occurs in the central airways and obscures the peameability change in the lung periphery. The radioaerosol deposition pattern and its effct on assessing the pulmonary epithelial permeability was studied. 99mTc-DTPA aerosol scintigraphy was performed in 47 patients with pulmonary fibrosis (PF), 12 patients with chronic obstructive pulmonary diseases (COPD), and 27 non-smoking and 17 smoking healthy volunteers. The scintigraphic images of the lungs were classified into 4 grades: 0=homogeneous distribution; 1=patchy distribution; 2=hot spots with partial defects; and 3= hot spots with little deposition in the lung field. The rate constant was used as a parameter for the permeability. The smokers and patients with PF showed increased kep values of 2.36±1.21%/min (mean±SD) and 2.49±1.29% min as compared with the nonsmokers with 0.94±0.27% min, respectively. The nonsmokers, smokers and 36 patients with PF were classified as deposition grade 0 or 1, suggesting good aerosol penetration to the lung periphery. All patients with COPD showed either grade 2 or 3 deposition. Aerosol deposition in the central airways can cause underestimation of the permeability because of the thicker lining layer in the bronchus than in the alveolus. In conclusion, the aerosol deposition pattern should be analyzed when the method is applied clinically to assess the permeability of the bronchiolo-alveolar epithelium. (author)

  6. Immunoscintigraphy using /sup 111/In-DTPA labeled monoclonal antibodies: Comparison between ETC and planar imaging

    Energy Technology Data Exchange (ETDEWEB)

    Happ, J.; Baum, R.P.; Frohn, J.; Weimer, B.; Hoer, G.; Halbsguth, A.; Lochner, B.; Brandhorst, I.

    1987-12-01

    The present study was done in order to examine if the use of /sup 111/In-DTPA-labeled MAb fragments in place of /sup 131/I-labeled MAb fragments increases the sensitivity of tomographic immunoscintigraphy to reach the level of that of planar imaging techniques. In 11 patients with various primary tumors, local recurrences or metastases (colorectal carcinoma (n=7), ovarian carcinoma (n=2), papillary thyroid carcinoma (n=1), undifferentiated carcinoma of the lung (n=1)), immuniscintigraphy (IS) was carried out using /sup 111/In-DTPA-labeled F(ab')/sub 2/ fragments of various MAbs (anti-CEA, OC 125, anti-hTG) and planar and tomographic imaging were compared intraindividually. By conventional diagnostic procedures, the presence of a tumor mass was confirmed (transmission computer tomography, ultrasound) or verified (/sup 131/I whole-body scintigraphy, histology) in all cases. Immunoscintigraphy was positive in 9 out of 11 cases by ECT and in 10 out of 11 cases by planar imaging. When using /sup 111/In-labeled MAb fragments, intraindividual comparison of ECT and planar imaging resulted in a similar sensitivity. The increased sensitivity of ECT using this tracer in contrast to /sup 131/I-labeled MAb fragments may be attributed to the fact that the physical properties of /sup 111/In are much more suitable for the gamma cameras most commonly used (single detector, 38'' crystal); using /sup 111/In-labelled MAb fragments, count rates sufficient for ECT can be obtained within a reasonable acquisition time. This allows to combine IS with the advantages of ECT regarding tumour localization and prevention of artefacts due to superposition of background.

  7. Effect of background region of interest and time-interval selection on glomerular filtration ratio estimation by percentage dose uptake of (99m)Tc-DTPA in comparison with (51)Cr-EDTA clearance in healthy cats.

    Science.gov (United States)

    Debruyn, Katrien; Vandermeulen, Eva; Saunders, Jimmy H; Dobbeleir, André A; Ham, Hamphrey R; Peremans, Kathelijne

    2013-08-01

    Evaluation of glomerular function is a useful part of the diagnostic approach in animals suspected of having renal disease. Time-interval and background region of interest (bg ROI) selection are determining factors when calculating the glomerular filtration ratio (GFR) based on percentage uptake of (99m)technetium-labelled diethylene triamine penta-acetic acid ((99m)Tc-DTPA). Therefore, three different time intervals (60-120 s, 120-180 s, 60-180 s) and three different bg ROIs (C-shape, caudolateral, cranial + caudal) were investigated. In addition, global GFRs based on percentage dose uptake of (99m)Tc-DTPA for the different time-intervals and bg ROIs were compared with the global GFR based on (51)chromium-ethylene diaminic tetra-acetic acid ((51)Cr-EDTA) plasma clearance in nine healthy European domestic shorthair cats. Paired Student's t-tests and linear regression analysis were used to analyse the data. Different time intervals seemed to cause significant variation (P <0.01) in absolute GFR values, regardless of the choice of bg ROI. Significant differences (P <0.01) between bg ROIs were only observed in the 120-180s time interval between the C-shape and cranial + caudal bg ROI, and between the caudolateral and cranial + caudal bg ROI. The caudolateral bg ROI in the 60-180 s time interval showed the highest correlation coefficient (r = 0.882) between (99m)Tc-DTPA and (51)Cr-EDTA, although a significant difference (P <0.05) was present between both techniques. PMID:23349527

  8. CFD Based Determination of Dynamic Stability Derivatives in Yaw for a Bird

    Institute of Scientific and Technical Information of China (English)

    M. A. Moelyadi; G. Sachs

    2007-01-01

    Dynamic yaw stability derivatives of a gull bird are determined using Computational Fluid Dynamics(CFD) method. Two kinds of motions are applied for calculating the dynamic yaw stability derivatives CNr and CNβ. The first one relates to a lateral translation and, separately, to a yaw rotation. The second one consists of a combined translational and rotational motion. To determine dynamic yaw stability derivatives, the simulation of an unsteady flow with a bird model showing a harmonic motion is performed. The flow solution for each time step is obtained by solving unsteady Euler equations based on a finite volume approach for a small reduced frequency. Then, an evaluation of unsteady forces and moments for one cycle is conducted using harmonic Fourier analysis. The results of the dynamic yaw stability derivatives for both simulations of the model show a good agreement.

  9. Preparation of {sup 166} Dy/{sup 166} Ho DTPA-bis biotin as a system of In vivo generator; Preparacion de {sup 166} Dy/{sup 166} Ho DTPA-bis biotina como un sistema de generador In vivo

    Energy Technology Data Exchange (ETDEWEB)

    Jimenez V, M.R

    2003-07-01

    The objective of this work was to synthesize the complex {sup 166} Dy/{sup 166} Ho - diethylen triamine pentaacetic-bis Biotin ({sup 166} Dy/{sup 166} Ho DTPA-bis Biotin) to evaluate its potential as a new radiopharmaceutical in directed radiotherapy. The Dysprosium-166 was obtained for neutron irradiation of {sup 164} Dy{sub 2}0{sub 3} in the TRIGA Mark III reactor. The labelled was carried out in aqueous solution to p H 8.0 for addition of {sup 166} Dy Cl{sub 3} to the diethylen triamine pentaacetic-{alpha}, {omega}-bis Biotin (DTPA-bis Biotin). The radiochemical purity was determined for HPLC and ITLC. The biological integrity of the marked biotin is evaluated by the biological recognition of the avidin for HPLC - molecular exclusion with and without avidin addition. The studies of stability in vitro were made in dilutions of saline solution to 0.9% and with human serum at 37 C incubated 1 and 24 hours. The complex {sup 166} Dy/{sup 166} Ho DTPA-bis Biotin was obtained with a radiochemical purity of 99.1 {+-} 0.6%. The biological recognition of the complex {sup 166} Dy/{sup 166} Ho DTPA-bis Biotin for the avidin it doesn't affect the labelling procedure. The studies in vitro demonstrated that the {sup 166} Dy/{sup 166} Ho DTPA-bis Biotin is stable after the dilution in saline solution and in human serum that there is not translocation of the one radionuclide subsequent son to the beta decay of the {sup 166} Dy that could produce the {sup 166} Ho{sup 3+} liberation. The studies of Biodistribution in healthy mice demonstrated that the one complex {sup 166} Dy/{sup 166} Ho DTPA-bis Biotin have a high renal distribution. In conclusion the radiolabelled biotin in this investigation has the appropriate properties to be used as an In vivo generator system stable for directed radiotherapy. (Author)

  10. Design of Chitosan and Its Water Soluble Derivatives-Based Drug Carriers with Polyelectrolyte Complexes

    Directory of Open Access Journals (Sweden)

    Qing-Xi Wu

    2014-12-01

    Full Text Available Chitosan, the cationic polysaccharide derived from the natural polysaccharide chitin, has been studied as a biomaterial for more than two decades. As a polycationic polymer with favorable properties, it has been widely used to form polyelectrolyte complexes with polyanions for various applications in drug delivery fields. In recent years, a growing number of studies have been focused on the preparation of polyelectrolyte complexes based on chitosan and its water soluble derivatives. They have been considered well-suited as biomaterials for a number of vital drug carriers with targeted/controlled release profiles, e.g., films, capsules, microcapsules. In this work, an overview highlights not only the favorable properties of chitosan and its water soluble derivatives but also the good performance of the polyelectrolyte complexes produced based on chitosan. Their various types of applications as drug carriers are reviewed in detail.

  11. Analytical derivation: An epistemic game for solving mathematically based physics problems

    Science.gov (United States)

    Bajracharya, Rabindra R.; Thompson, John R.

    2016-06-01

    Problem solving, which often involves multiple steps, is an integral part of physics learning and teaching. Using the perspective of the epistemic game, we documented a specific game that is commonly pursued by students while solving mathematically based physics problems: the analytical derivation game. This game involves deriving an equation through symbolic manipulations and routine mathematical operations, usually without any physical interpretation of the processes. This game often creates cognitive obstacles in students, preventing them from using alternative resources or better approaches during problem solving. We conducted hour-long, semi-structured, individual interviews with fourteen introductory physics students. Students were asked to solve four "pseudophysics" problems containing algebraic and graphical representations. The problems required the application of the fundamental theorem of calculus (FTC), which is one of the most frequently used mathematical concepts in physics problem solving. We show that the analytical derivation game is necessary, but not sufficient, to solve mathematically based physics problems, specifically those involving graphical representations.

  12. Synthesis and antitumor evaluation of arctigenin derivatives based on antiausterity strategy.

    Science.gov (United States)

    Kudou, Naoki; Taniguchi, Akira; Sugimoto, Kenji; Matsuya, Yuji; Kawasaki, Masashi; Toyooka, Naoki; Miyoshi, Chika; Awale, Suresh; Dibwe, Dya Fita; Esumi, Hiroyasu; Kadota, Shigetoshi; Tezuka, Yasuhiro

    2013-02-01

    A series of new (-)-arctigenin derivatives with variably modified O-alkyl groups were synthesized and their preferential cytotoxicity was evaluated against human pancreatic cancer cell line PANC-1 under nutrient-deprived conditions. The results showed that monoethoxy derivative 4i (PC(50), 0.49 μM), diethoxy derivative 4h (PC(50), 0.66 μM), and triethoxy derivative 4m (PC(50), 0.78 μM) showed the preferential cytotoxicities under nutrient-deprived conditions, which were identical to or more potent than (-)-arctigenin (1) (PC(50), 0.80 μM). Among them, we selected the triethoxy derivative 4m and examined its in vivo antitumor activity using a mouse xenograft model. Triethoxy derivative 4m exhibited also in vivo antitumor activity with the potency identical to or slightly more than (-)-arctigenin (1). These results would suggest that a modification of (-)-arctigenin structure could lead to a new drug based on the antiausterity strategy. PMID:23287053

  13. Evaluation of Ferrocene Derivatives as Burn Rate Modifiers in AP/HTPB-Based Composite Propellants

    Directory of Open Access Journals (Sweden)

    G. M. Gore

    1999-04-01

    Full Text Available Some ferrocene derivatives like 2,4-dinitrophenylhydrazine derivative of acetyl ferrocene, 1-pyrrolidinylmethyl ferrocene, di-ter-butyl ferrocene and 1,3-diferrocenyl-l-butene (DFB have been synthesised and characterised by infrared, nuclear magnetic resonance, ultraviolet, iron content, etc. To study the effect of their incorporation on performance, ammonium perchlorate/hydroxyl-terminated polybutadiene-based composite propellants containing these derivatives have been prepared and studied for burn rates, tensile strength and percentage elongation followed by their static test evaluation. A comparison of the properties of propellant containing solid and liquid ferrocene derivatives has been made with those containing Fe/sub 2/O/sub 3/ and n-butyl ferrocene, respectively. The data clearly indicates that these ferrocene derivatives are better than Fe/sub 2/O/sub 3/ and n-butyl errocene. Also, DFB is the best among these derivatives. Like composite propellants, DFB increases burn rate in fuel-rich propellants also.

  14. Synthesis and NO production inhibition of novel Mannich base derivatives of irisolidone

    Institute of Scientific and Technical Information of China (English)

    Jiao Wang; Ying Zhong; Lu Liu; Xin Quan Ji; Zhi Guo Zhang

    2008-01-01

    Five new C-8 Mannich base derivatives of irisolidone 2a-2e were synthesized and their nitric oxide (NO) production inhibitory activity was evaluated.Compounds 2a,2b,2c and 2e displayed stronger activities in vitro than the parent compound irisolidone.

  15. Empirically Derived Consequences: A Data-Based Method for Prescribing Treatments for Destructive Behavior.

    Science.gov (United States)

    Fisher, Wayne; And Others

    1994-01-01

    This study used a data-based assessment to identify reinforcers and punishers for successful treatment of two children with severe destructive behaviors. Results suggested that empirically derived consequences may be useful in decreasing destructive behavior when a functional assessment is inconclusive or suggests that internal stimuli are…

  16. Intraindividual comparison of T1 relaxation times after gadobutrol and Gd-DTPA administration for cardiac late enhancement imaging

    Energy Technology Data Exchange (ETDEWEB)

    Doeblin, Patrick, E-mail: Patrick.doeblin@charite.de [Department of Cardiology, Charité – Universitätsmedizin Berlin, Charité Campus Benjamin Franklin, Berlin (Germany); Schilling, Rene, E-mail: rene.schilling@charite.de [Department of Radiology, Charité – Universitätsmedizin Berlin, Charité Campus Mitte, Berlin (Germany); Wagner, Moritz, E-mail: moritz.wagner@charite.de [Department of Radiology, Charité – Universitätsmedizin Berlin, Charité Campus Mitte, Berlin (Germany); Luhur, Reny, E-mail: renyluhur@yahoo.com [Department of Radiology, Charité – Universitätsmedizin Berlin, Charité Campus Mitte, Berlin (Germany); Huppertz, Alexander, E-mail: alexander.huppertz@charite.de [Department of Radiology, Charité – Universitätsmedizin Berlin, Charité Campus Mitte, Berlin (Germany); Imaging Science Institute, Charité, Berlin (Germany); Hamm, Bernd, E-mail: bernd.hamm@charite.de [Department of Radiology, Charité – Universitätsmedizin Berlin, Charité Campus Mitte, Berlin (Germany); Taupitz, Matthias, E-mail: matthias.taupitz@harite.de [Department of Radiology, Charité – Universitätsmedizin Berlin, Charité Campus Mitte, Berlin (Germany); and others

    2014-04-15

    Purpose: To evaluate T1-relaxation times of chronic myocardial infarction (CMI) using gadobutrol and gadopentetate dimeglumine (Gd-DTPA) over time and to determine the optimal imaging window for late enhancement imaging with both contrast agents. Material and methods: Twelve patients with CMI were prospectively included and examined on a 1.5 T magnetic resonance (MR) system using relaxivity-adjusted doses of gadobutrol (0.15 mmol/kg) and Gd-DTPA (0.2 mmol/kg) in random order. T1-relaxation times of remote myocardium (RM), infarcted myocardium (IM), and left ventricular cavity (LVC) were assessed from short-axis TI scout imaging using the Look–Locker approach and compared intraindividually using a Wilcoxon paired signed-rank test (α < 0.05). Results: Within 3 min of contrast agent administration (CA), IM showed significantly lower T1-relaxation times than RM with both contrast agents, indicating beginning cardiac late enhancement. Differences between gadobutrol and Gd-DTPA in T1-relaxation times of IM and RM were statistically not significant through all time points. However, gadobutrol led to significantly higher T1-relaxation times of LVC than Gd-DTPA from 6 to 9 min (220 ± 15 ms vs. 195 ± 30 ms p < 0.01) onwards, resulting in a significantly greater ΔT1 of IM to LVC at 9–12 min (−20 ± 35 ms vs. 0 ± 35 ms, p < 0.05) and 12–15 min (−25 ± 45 ms vs. −10 ± 60 ms, p < 0.05). Using Gd-DTPA, comparable ΔT1 values were reached only after 25–35 min. Conclusion: This study indicates good delineation of IM to RM with both contrast agents as early as 3 min after administration. However, we found significant differences in T1 relaxation times with greater ΔT1 IM–LVC using 0.15 mmol/kg gadobutrol compared to 0.20 mmol/kg Gd-DTPA after 9–15 min post-CA suggesting earlier differentiability of IM and LVC using gadobutrol.

  17. Gd-DTPA enhanced MR-follow-up of autologous patellar ligament transplants for anterior cruciate ligament repair. Gadolinium-DTPA gestuetzte MRT zur Beurteilung des Einheilens von autologen Ligamentum-patellae-Transplantaten als vorderer Kreuzbandersatzplastik

    Energy Technology Data Exchange (ETDEWEB)

    Tosch, U.; Felix, R. (Freie Univ. Berlin (Germany). Strahlenklinik und Poliklinik); Hertel, P.; Bernard, M. (Freie Univ. Berlin (Germany). Unfallchirurgische Abt. Martin-Luther Krankenhaus); Lais, E. (Freie Univ. Berlin (Germany). Unfallchirurgische Abt. Klinikum Rudolf Virchow)

    1993-01-01

    Eighteen patients with autologous patellar tendon transplant for reconstruction the anterior cruciate ligament were examined postoperatively. The time between operation and MR study was up to 3 weeks in ten patients, between 3 and 35 weeks in three patients and more than 35 weeks in five patients. In all patients, Gd-DTPA enhancement on the surface of the ligamentous transplant was visualized. This enhancement was between 1 and 10 mm thick. In all patients, complete stability of the knee joint was postoperatively. In 15 patients achieved flexion and extension were normal at the postoperative investigation. Three patients had a limitation of knee movement. In these MR studies, significant hyperplasia of the synovial membrane of the entire joint was diagnosed, explaining the persisting problems. Gd-DTPA-enhanced MR of the knee joint allows non-invasive documentation of remodelling following a patellar ligament transplant and possible complications. (orig.).

  18. Preliminary Clinical Experience of 111In-DTPA-octreotide in Somatostatin Receptor-positive Tumors%111In-DTPA-octreotide在生长抑素受体表达阳性肿瘤的初步临床应用

    Institute of Scientific and Technical Information of China (English)

    李方; 陈黎波; 张少华; 杜延荣; 陈方

    2003-01-01

    目的:评价自制111In-DTPA-octreotide在生长抑素受体表达阳性肿瘤显像中的应用价值.材料和方法:36例患者行111In-DTPA-octreotide显像.包括神经内分泌性肿瘤15例,非神经内分泌性肿瘤14例以及7例肺部良性病变.静注111In-DTPA-octreotide 185Mq后于4、24h行局部及全身显像,部分病例行断层显像.结果:13例神经内分泌肿瘤、12例非神经内分泌性肿瘤和3例肺部良性病变显像阳性.2例神经内分泌肿瘤、2例非神经内分泌性肿瘤和4例肺部良性病变显像阴性.肺癌患者显像均为阳性.本研究灵敏度86.2%,特异性57.1%,准确性80.6%.结论:111In-DTPA-octreotide对生长抑素受体表达阳性肿瘤肿瘤显像均有较高的诊断价值,但肺部良恶性病变的特异性不高.

  19. A new method based on Markov chains for deriving SB2 orbits directly from their spectra

    CERN Document Server

    Salomon, J -B; Guillout, P; Halbwachs, J -L; Arenou, F; Famaey, B; Lebreton, Y; Mazeh, T; Pourbaix, D; Tal-Or, L

    2012-01-01

    We present a new method to derive orbital elements of double-lined spectroscopic binaries (SB2). The aim is to have accurate orbital parameters of a selection of SB2 in order to prepare the exploitation of astrometric Gaia observations. Combined with our results, they should allow one to measure the mass of each star with a precision of better than 1%. The new method presented here consists of using the spectra at all epochs simultaneously to derive the orbital elements without templates. It is based on a Markov chain including a new method for disentangling the spectra.

  20. Synthesis, crystal structures and photoluminescence of anthracen- and pyrene-based coumarin derivatives.

    Science.gov (United States)

    Zhang, Hui; Tong, Hao; Zhao, Yuling; Yu, Tianzhi; Zhang, Peng; Li, Jianfeng; Fan, Duowang

    2015-11-01

    Two new anthracen- and pyrene-based coumarin derivatives, 3-(4-(anthracen-10-yl)phenyl)coumarin (4) and 3-(4-(pyrene-1-yl)phenyl)coumarin (5), were synthesized and characterized by FT-IR, (1)H NMR, element analysis and single crystal X-ray crystallography. The UV-vis absorption and photoluminescence spectra of these coumarin derivatives were investigated. The results show that compound 4 and 5 exhibit blue and blue-green emissions, respectively, under ultraviolet light excitation. Compared with the compound 4, the emission peak of compound 5 was bathochromically shifted by about 80 nm due to the more planar structure and larger π-conjugation.

  1. Isatin based thiosemicarbazone derivatives as potential bioactive agents: Anti-oxidant and molecular docking studies

    Science.gov (United States)

    Haribabu, J.; Subhashree, G. R.; Saranya, S.; Gomathi, K.; Karvembu, R.; Gayathri, D.

    2016-04-01

    A new series of isatin based thiosemicarbazones has been synthesized from benzylisatin and unsubstituted/substituted thiosemicarbazides (1-5). The synthesized compounds were characterized by elemental analyses, and UV-Visible, FT-IR, 1H &13C NMR and mass spectroscopic techniques. Three dimensional molecular structure of three compounds (1, 3 and 4) was determined by single crystal X-ray crystallography. Anti-oxidant activity of the thiosemicarbazone derivatives showed their excellent scavenging effect against free radicals. In addition, all the compounds showed good anti-haemolytic activity. In silico molecular docking studies were performed to screen the anti-inflammatory and anti-tuberculosis properties of thiosemicarbazone derivatives.

  2. A Comparative Study of 99mTc octreotide and 111In DTPA octreotide Receptor Imaging for Tumor Diagnosis%Tc octreotide和 In DTPA octreotide受体显像诊断肿瘤的比较研究

    Institute of Scientific and Technical Information of China (English)

    管昌田; 梁正路; 管玫; 李云春

    2001-01-01

    [目的 ]对比研究 99mTc octreotide和 111In DTPA octreotide受体显像对肿瘤诊断的效能 . [方法 ]采用自行制备的 111In-DTPA-octreotide和 99mTc octreotide分别对 4例和 3例正常对照以及对 18例和 12例肿瘤患者进行显像 , 以发现局灶性异常放射性浓集为阳性 , 比较其对肿瘤诊断的效能 . [结果 ]两种显像剂的正常图像、显像质量和对生长抑素受体 (SMS R)阳性肿瘤的诊断效能无显著差异 , 两种显像剂显像结果均与临床最后诊断相符合 . [结论 ]99mTc octreotide是一种价廉易得的优良的肿瘤 SMS R显像剂 , 在临床上可以替代价格高昂不易获得的 111In DTPA octreotide进行肿瘤显像 .

  3. Derivative-based global sensitivity measures: general links with Sobol' indices and numerical tests

    CERN Document Server

    Matieyendou, Matieyendou; Popelin, Anne-Laure; Gamboa, Fabrice

    2012-01-01

    The estimation of variance-based importance measures (called Sobol' indices) of the input variables of a numerical model can require a large number of model evaluations. It turns to be unacceptable for huge model involving a large number of input variables (typically more than ten). Recently, Sobol and Kucherenko have proposed the Derivative-based Global Sensitivity Measures (DGSM), defined as the integral of the squared derivatives of the model output, showing that it can help to solve the problem of dimensionality in some cases. We provide a general inequality link between DGSM and total Sobol' indices for input variables belonging in the class of Boltzmann probability measures, extending the previous results of Sobol and Kucherenko for uniform and normal measures. The special case of log-concave measures is also described. This link provides a DGSM-based maximal bound for the total Sobol indices. Numerical tests show the performance of the bound and its usefulness in practice.

  4. A chaotic communication system of improved performance based on the Derivative-free nonlinear Kalman filter

    Science.gov (United States)

    Rigatos, Gerasimos

    2016-07-01

    The Derivative-free nonlinear Kalman Filter is used for developing a communication system that is based on a chaotic modulator such as the Duffing system. In the transmitter's side, the source of information undergoes modulation (encryption) in which a chaotic signal generated by the Duffing system is the carrier. The modulated signal is transmitted through a communication channel and at the receiver's side demodulation takes place, after exploiting the estimation provided about the state vector of the chaotic oscillator by the Derivative-free nonlinear Kalman Filter. Evaluation tests confirm that the proposed filtering method has improved performance over the Extended Kalman Filter and reduces significantly the rate of transmission errors. Moreover, it is shown that the proposed Derivative-free nonlinear Kalman Filter can work within a dual Kalman Filtering scheme, for performing simultaneously transmitter-receiver synchronisation and estimation of unknown coefficients of the communication channel.

  5. Prediction on the inhibition ratio of pyrrolidine derivatives on matrix metalloproteinase based on gene expression programming.

    Science.gov (United States)

    Li, Yuqin; You, Guirong; Jia, Baoxiu; Si, Hongzong; Yao, Xiaojun

    2014-01-01

    Quantitative structure-activity relationships (QSAR) were developed to predict the inhibition ratio of pyrrolidine derivatives on matrix metalloproteinase via heuristic method (HM) and gene expression programming (GEP). The descriptors of 33 pyrrolidine derivatives were calculated by the software CODESSA, which can calculate quantum chemical, topological, geometrical, constitutional, and electrostatic descriptors. HM was also used for the preselection of 5 appropriate molecular descriptors. Linear and nonlinear QSAR models were developed based on the HM and GEP separately and two prediction models lead to a good correlation coefficient (R (2)) of 0.93 and 0.94. The two QSAR models are useful in predicting the inhibition ratio of pyrrolidine derivatives on matrix metalloproteinase during the discovery of new anticancer drugs and providing theory information for studying the new drugs. PMID:24971318

  6. Prediction on the Inhibition Ratio of Pyrrolidine Derivatives on Matrix Metalloproteinase Based on Gene Expression Programming

    Directory of Open Access Journals (Sweden)

    Yuqin Li

    2014-01-01

    Full Text Available Quantitative structure-activity relationships (QSAR were developed to predict the inhibition ratio of pyrrolidine derivatives on matrix metalloproteinase via heuristic method (HM and gene expression programming (GEP. The descriptors of 33 pyrrolidine derivatives were calculated by the software CODESSA, which can calculate quantum chemical, topological, geometrical, constitutional, and electrostatic descriptors. HM was also used for the preselection of 5 appropriate molecular descriptors. Linear and nonlinear QSAR models were developed based on the HM and GEP separately and two prediction models lead to a good correlation coefficient (R2 of 0.93 and 0.94. The two QSAR models are useful in predicting the inhibition ratio of pyrrolidine derivatives on matrix metalloproteinase during the discovery of new anticancer drugs and providing theory information for studying the new drugs.

  7. Integration of Color and Local Derivative Pattern Features for Content-Based Image Indexing and Retrieval

    Science.gov (United States)

    Vipparthi, Santosh Kumar; Nagar, Shyam Krishna

    2015-09-01

    This paper presents two new feature descriptors for content based image retrieval (CBIR) application. The proposed two descriptors are named as color local derivative patterns (CLDP) and inter color local derivative pattern (ICLDP). In order to reduce the computational complexity the uniform patterns are applied to both CLDP and ICLDP. Further, uniform CLDP (CLDPu2) and uniform ICLDP (ICLDPu2) are generated respectively. The proposed descriptors are able to exploit individual (R, G and B) spectral channel information and co-relating pair (RG, GB, BR, etc.) of spectral channel information. The retrieval performances of the proposed descriptors (CLDP and ICLDP) are tested by conducting two experiments on Corel-5000 and Corel-10000 benchmark databases. The results after investigation show a significant improvement in terms of precision, average retrieval precision (ARP), recall and average retrieval rate (ARR) as compared to local binary patterns (LBP), local derivative patterns (LDP) and other state-of-the-art techniques for image retrieval.

  8. Gd-DTPA enhanced MR-follow-up of autologous patellar ligament transplants for anterior cruciate ligament repair

    International Nuclear Information System (INIS)

    Eighteen patients with autologous patellar tendon transplant for reconstruction the anterior cruciate ligament were examined postoperatively. The time between operation and MR study was up to 3 weeks in ten patients, between 3 and 35 weeks in three patients and more than 35 weeks in five patients. In all patients, Gd-DTPA enhancement on the surface of the ligamentous transplant was visualized. This enhancement was between 1 and 10 mm thick. In all patients, complete stability of the knee joint was postoperatively. In 15 patients achieved flexion and extension were normal at the postoperative investigation. Three patients had a limitation of knee movement. In these MR studies, significant hyperplasia of the synovial membrane of the entire joint was diagnosed, explaining the persisting problems. Gd-DTPA-enhanced MR of the knee joint allows non-invasive documentation of remodelling following a patellar ligament transplant and possible complications. (orig.)

  9. [Does intravenous gadolinium-DTPA administration have advantages in magnetic resonance imaging of acute injuries or chronic damage to the knee joint?].

    Science.gov (United States)

    Jerosch, J; Castro, W H; Müller, U; Assheuer, J

    1994-12-01

    79 patients with acute or chronic lesions of the knee were evaluated by MRI prior to and after application of Gd-DTPA. The MRI examination was performed by a 1.0 tesla imager with SE as well as FEDIF sequences. These MR studies were compared prior to and after intravenous Gd-DTPA application, focusing on the visibility and the definition of a possible lesion, and scored with a 3-point score. Statistic analysis and case analysis revealed that in patients with meniscus degeneration without a tear, Gd application yields no additional diagnostic information. However, in patients with meniscus tears Gd-DTPA significantly facilitates the definition of the lesion. Furthermore, Gd-DTPA makes differentiation possible between the synovial fluid and the synovial membrane. Whereas in cases with capsule or collateral ligament tears Gd-DTPA facilitates the documentation of the lesion, we found no advantage in using Gd-DPTA in patients with ACL tears. In patients with chondropathia patellae Gd-DTPA application supports the visualization of the secondary synovial reaction.

  10. Aqueous complexation of thorium(IV), uranium(IV), neptunium(IV), plutonium(III/IV), and cerium(III/IV) with DTPA.

    Science.gov (United States)

    Brown, M Alex; Paulenova, Alena; Gelis, Artem V

    2012-07-16

    Aqueous complexation of Th(IV), U(IV), Np(IV), Pu(III/IV), and Ce(III/IV) with DTPA was studied by potentiometry, absorption spectrophotometry, and cyclic voltammetry at 1 M ionic strength and 25 °C. The stability constants for the 1:1 complex of each trivalent and tetravalent metal were calculated. From the potentiometric data, we report stability constant values for Ce(III)DTPA, Ce(III)HDTPA, and Th(IV)DTPA of log β(101) = 20.01 ± 0.02, log β(111) = 22.0 ± 0.2, and log β(101) = 29.6 ± 1, respectively. From the absorption spectrophotometry data, we report stability constant values for U(IV)DTPA, Np(IV)DTPA, and Pu(IV)DTPA of log β(101) = 31.8 ± 0.1, 32.3 ± 0.1, and 33.67 ± 0.02, respectively. From the cyclic voltammetry data, we report stability constant values for Ce(IV) and Pu(III) of log β(101) = 34.04 ± 0.04 and 20.58 ± 0.04, respectively. The values obtained in this work are compared and discussed with respect to the ionic radius of each cationic metal.

  11. Structure-based designing of sordarin derivative as potential fungicide with pan-fungal activity.

    Science.gov (United States)

    Chakraborty, Biprashekhar; Sejpal, Nikunjkumar Vinodray; Payghan, Pavan V; Ghoshal, Nanda; Sengupta, Jayati

    2016-05-01

    Fungal infections have become a significant problem for immunosuppressed patients. Sordarin, a promising fungicidal agent, inhibits fungal protein synthesis by impairing elongation factor-2 (eEF2) function. Intriguingly, despite high sequence similarity among eEF2s from different species, sordarin has been shown to inhibit translation specifically in certain fungi while unable to do so in some other fungal species (e.g. Candida parapsilosis and Candida lusitaniae). The sordarin binding site on eEF2 as well as its mechanism of action is known. In a previous study, we have detailed the interactions between sordarin and eEF2 cavities from different fungal species at the molecular level and predicted the probable cause of sordarin sensitivity. Guided by our previous analysis, we aimed for computer-aided designing of sordarin derivatives as potential fungicidal agents that still remain ineffective against human eEF2. We have performed structural knowledge-based designing of several sordarin derivatives and evaluated predicted interactions of those derivatives with the sordarin-binding cavities of different eEF2s, against which sordarin shows no inhibitory action. Our analyses identify an amino-pyrrole derivative as a good template for further designing of promising broad-spectrum antifungal agents. The drug likeness and ADMET prediction on this derivative also supports its suitability as a drug candidate.

  12. Detection of alveolar epithelial injury by 99mTc-DTPA radioaerosol inhalation lung scan following blunt chest trauma

    International Nuclear Information System (INIS)

    DTPA clearance rate is a reliable index of alveolar epithelial permeability, and is a highly sensitive marker of pulmonary epithelial damage, even of mild degree. In this study, 99mTc-DTPA aerosol inhalation scintigraphy was used to assess the pulmonary epithelial membrane permeability and to investigate the possible application of this permeability value as an indicator of early alveolar or interstitial changes in patients with blunt chest trauma. A total of 26 patients was chest trauma (4 female, 22 male, 31-80 yrs, mean age; 53±13 yrs) who were referred to the emergency department in our hospital participated in this study. Technetium-99m diethylene triamine pentaacetic acid (DTPA) aerosol inhalation scintigraphy was performed on the first and thirtieth days after trauma. Clearance half times (T1/2) were calculated by placing a mono-exponential fit on the curves. Penetration index (PI) was calculated on the first-minute image. On the first day, mean T1/2 value of the whole lung was 63±19 minutes (min), and thirtieth day mean T1/2 value was 67±21 min. On the first day, mean PI values of the lung and 30th day mean PI value were 0.60±0.05, and 0.63 ±0.05, respectively. Significant changes were observed in radioaerosol clearance and penetration indices. Following chest trauma, clearance of 99mTc-DTPA increased owing to breakdown of the alveolar-capillary barrier. This increase in the epithelial permeability of the lung appears to be an early manifestation of lung disease that may lead to efficient therapy in the early phase. (author)

  13. Exercise induced renal dysfunction studied by sup(99m)Tc-DTPA in hypertensives and normotensive controls

    International Nuclear Information System (INIS)

    Clorius and Schmidlin reported that the disturbance of renal hippurate transport during exercise is a phenomenon specific to patients with hypertension. Our study with sup(99m)Tc-DTPA revealed that exercise induced renal dysfunction was observed not only in hypertensives (H) but also in normotensive controls (N). sup(99m)Tc-DTPA was intravenously injected at rest and during bicycle ergometric stress to 14 H and 14 N in sitting position. Serial dynamic renal images were taken, of which data were simultaneously stored in a data processor for later analysis. The renogram was drawn from the ROI on each kidney. Peak time (PT) of secretory phase and radioisotope retention rate (RR) at 10 minutes were the parameters compared between rest and exercise. GFR of each kidney was calculated from % renal uptake of sup(99m)Tc-DTPA (method reported by Gates). Blood samples were obtained at rest and at the end of exercise for the measurement of aldosterone (ALD), plasma renin activity (PRA) and catecholamines (A, NA). Exercise caused significant, prolongation of PT and increase in RR (10 min. counts/peak counts) both in H and N. GFR (ml/min.) during exercise was lower than at rest in both H (80.7 +- 22.4 vs. 93.8 +- 16.9, p < 0.02) and N (84.4 +- 17.3 vs. 102.1 +- 15.1, p < 0.01). ALD, PRA, A and NA were all elevated during exercise both in H and N. None of the rest-exercise differences significantly differed between H and N. Our data indicate the exercise induced renal dysfunction demonstrated by sup(99m)Tc-DTPA renograms is not specific to H but can also be observed in N, which may have resulted from changes common to H and N, as indicated by GFR and humoral factors. (author)

  14. Correlation of Tc-99m DTPA aerosol ventilation studies with pulmonary function testing in patients with cystic fibrosis

    International Nuclear Information System (INIS)

    The authors compared 68 Tc-99m DTPA aerosol ventilation studies with pulmonary function tests results in 18 patients with cystic fibrosis. The ventilation studies were evaluated blindly for (1) number of nonventilated pulmonary segments, (2) number of bronchial deposition foci, and (3) subjective overall improvement, lack of change, or worsening from the previous study. Finding 1 correlated with oxygen saturation, the percentage of predicted forced vital capacity (FVC), and the ratio of forced expiratory volume (1 sec)

  15. The fate of EDTA and DTPA in aquatic environments receiving waste water from two pulp and paper mills

    Energy Technology Data Exchange (ETDEWEB)

    Remberger, M.; Svenson, Anders

    1997-10-01

    To evaluate the fate of the complexing agents in receiving waters, two basic questions have been addressed: (i) are EDTA and DTPA found in the aquatic environment after discharge into receiving waters and (ii) are they photolytically converted. Two mills, one pulp mill localized at a fresh water lake and one pulp and paper mill at a brackish water were investigated, both mills using bleaching technologies with EDTA and DTPA as complexing agents. Samples were collected at the discharge point and along a gradient in the receiving waters at two occasions: summer at solstice and winter with low light intensity. Samples were taken from surface water, an intermediate depth, and bottom water. A new analytical method was applied, which made it possible to quantify the analytes at sub-{mu}g/l level. The complexing agents EDTA and DTPA and their primary degradation products were detected in the effluent and the receiving waters in the vicinity of the mills. DTPA and the degradation products could be detected a few kilometers from the effluent point while EDTA could be detected in more remote locations at fairly constant concentrations. The absorption of light in the sun spectrum in the water columns of the receiving waters was studied at different localities and during summer and winter conditions. The theoretical photochemical half-life of the ferric complex of EDTA in the surface layer of a central Swedish lake was confirmed. Analysis of EDTA in samples of receiving waters after photolytic treatment showed however, that a large portion of the complexing agent was unaffected by the treatment, indicating that most of the EDTA was complexed with other metals. EDTA in brackish water samples was unaffected by the photolytic treatment upon addition of excess ferric ions, except in winter close to the discharge point. The ease by which the ferric complexes are photochemically converted in ideal conditions seems to be hampered in receiving waters. 42 refs, 16 figs, 2 tabs

  16. Intracoronary delivery of Gd-DTPA and Gadophrin-2 for determination of myocardial viability with MR imaging

    Energy Technology Data Exchange (ETDEWEB)

    Ni, Y.; Bosmans, H.; Miao, Y.; Bogaert, J.; Dymarkowski, S.; Yu, J.; Baert, A.L.; Marchal, G. [Dept. of Radiology, University Hospitals, Catholic University Leuven (Belgium); Pislaru, C.; Pislaru, S.; Werf, F. van de [Dept. of Cardiology, University Hospitals, Catholic University Leuven (Belgium); Semmler, W. [Inst. fuer Diagnostikforschung GmbH, Berlin (Germany)

    2001-05-01

    The aim of this study was to compare intracoronary (i. c.) administration of Gadophrin-2, a necrosis-avid contrast agent (NACA), and nonspecific agent Gd-DTPA for determination of myocardial viability (MV) in acute myocardial infarction (AMI) with MRI. Reperfused AMI was induced in 12 dogs by transcatheter balloon occlusion of coronary artery. In 6 dogs each, Gd-DTPA at 0.1 mmol/kg or Gadophrin-2 at 0.005 mmol/kg was administered into coronary artery by fast bolus (n = 3) or slow infusion (n = 3). Serial ECG-triggered cardiac MRI of T1-weighted segmented turbo fast low-angle shot (FLASH) sequence was conducted and compared with triphenyltetrazolium chloride (TTC) histochemical staining. The contrast ratio and infarct size were quantified and analysed statistically. No cardiovascular side effects were found with local delivery of both agents. After i. c. administration, Gadophrin-2 induced a strong (CR {>=} 1.78) and persistent ({>=} 10 h) contrast enhancement of infarcted region. The infarct size defined with Gadophrin-2 was almost identical to that with TTC staining throughout the postcontrast period. With a dose 20 times higher, Gd-DTPA also strongly enhanced infarct-to-normal contrast; however, the enhancement diminished with time, i. e. from early strong to later faint enhancement and eventual loss of contrast. The delineated infarct size was also unstable, i. e. from early overestimation to later underestimation and eventual disappearance of the enhanced infarct. In combination with PTCA procedure, i. c. administration of MRI contrast agents may prove useful for post-procedure verification of diagnosis. The NACA-enhanced MRI may serve as an in vivo surrogate of postmortem histochemical staining for determination of MV. Although applicable in clinical setting, cardiac MRI with nonspecific Gd-DTPA is less reliable and should be performed within less than 1 h after contrast. (orig.)

  17. Quantitative evaluation of enhancement patterns in focal solid liver lesions with Gd-EOB-DTPA-enhanced MRI.

    Directory of Open Access Journals (Sweden)

    Michael Haimerl

    Full Text Available PURPOSE: The objective was to investigate the dynamic enhancement patterns in focal solid liver lesions after the administration of gadolinium ethoxybenzyl diethylenetriamine pentaacetic acid (Gd-EOB-DTPA by means of dynamic magnetic resonance imaging (MRI including hepatobiliary phase (HP images 20 min after Gd-EOB-DTPA administration. MATERIALS AND METHODS: Non-enhanced T1/T2-weighted as well as dynamic magnetic resonance (MR images during the arterial phase (AP, the portal venous phase (PVP, the late phase (LP, and the HP (20 min were obtained from 83 patients (54 male, 29 female, mean age 62.01 years with focal solid liver lesions. MRI was conducted by means of a 1.5-T system for 63 patients with malignant liver lesions (HCCs: n = 34, metastases: n = 29 and for 20 patients with benign liver lesions (FNH lesions: n = 14, hemangiomas: n = 3, adenomas: n = 3. For quantitative analysis, signal-to-noise ratios (SNR, contrast enhancement ratios (CER, lesion-to-liver contrast ratios (LLC, and signal intensity (SI ratios were measured. RESULTS: The SNR of liver parenchyma significantly increased in each dynamic phase after Gd-EOB-DTPA administration compared to the SNR of non-enhanced images (p<0.001. The CER of HCCs and metastases significantly decreased between LP and HP images (p = 0.0011, p<0.0001. However, FNH lesions did not show any significant difference, whereas an increased CER was found in hemangiomas. The mean LLCs of FNH lesions were significantly higher than those of HCCs and metastases. The LLC values of hemangiomas remained negative during the entire time course, whereas the LLC of adenomas indicated hyperintensity from the AP to the LP. Furthermore, adenomas showed hypointensity in HP images. CONCLUSION: Gd-EOB-DTPA-enhanced MRI may help diagnose focal solid liver lesions by evaluating their enhancement patterns.

  18. Dynamic 99mTc-DTPA radioaerosol lung scanning for the evaluation of alveolar-capillary barrier permeability

    International Nuclear Information System (INIS)

    Pulmonary clearance of small droplet 99mTc-DTPA radioaerosol was studied in 100 patients (12 normal subjects, N; 10 asymptomatic healthy smoker, FA; 31 patients with interstitial lung diseases, IP; 47 patients with chronic obstructive lung disease, BPCO). The first seven minutes of clearance were described with the function At=Ao*exp(-K*t) and the time constant K was considered representative of the 99mTc-DTPA clearance rate and hence of the alveolar-capillary barrier permeability. Groups FA, IP and BPCO showed a significant (p99mTc-DTPA dynamic lung scanning is an easy, non-invasive method to assess derangements of alveolar-capillary barrier permeability secondary to epithelial damage; 2) permeability increase is a very early effect of cigarette smoke damafe to the epithelium; 3) other mechanisms of epithelial injury are present in diffuse lung disease; 4) while the clinical role of this new pathophysiological test is not yet clear, it is likely that it may become a very early marker of pulmonary epithelial damage in diffuse lung disease

  19. The clinical value of glomerular filtration rate with 99Tcm-DTPA on living kidney transplantation donor

    International Nuclear Information System (INIS)

    Objective: To determine the normal reference range of glomerular filtration rate (GFR) in different ages with 99Tcm-DTPA renal dynamic imaging on living kidney transplantation donor, and to evaluate the clinical value of GFR in living kidney transplantation. Methods: 99Tcm-DTPA renal dynamic imaging was performed in 300 patients on living kidney transplantation donor. The image was processed according to Gates' method to obtain GFR. The normal reference range of GFR was obtained in different ages and the relationship between GFR and gender, age and body mass index was also analyzed. Results: The left, right and total renal GFR of 300 living kidney transplantation donors were 49.25±10.34 ml/min. 49.27±9.69 ml/min and 98.52±19.03 ml/min, respectively. The GFR in the group of age 4 0 was higher significantly than that of age ≥50 (P 0.05). The study of logistic regression showed that the age was the only important impact factor on GFR. Conclusions: GFR obtained by 99Tcm-DTPA is simple and reliable, which can be used to accurately assess the individual renal filterability and the urinary drainage function, This affords an useful method on screening the living relative kidney transplantation donor. (authors)

  20. Clearance of inhaled sup(99m)Tc DTPA from regions of the lung recently affected by pulmonary embolus

    International Nuclear Information System (INIS)

    Pulmonary emboli lead to regional limitation of pulmonary artery perfusion, often without affecting distribution of ventilation. We have studied the effect of this regional reduction of pulmonary artery perfusion on the integrity of epithelium of alveoli (and possibly bronchioli). Integrity of alveolar epithelium was assessed by measuring regional rates of clearance from lung to blood of an inhaled aerosol of a small molecular weight solute, sup(99m)Tc DTPA (technetium-99m diethylene-triamine-penta-acetate). Ten patients with pulmonary emboli were studied, where the diagnosis was made from 'mismatching' seen on ventilation (V) and perfusion (Q) lung scintigraphy. Rates of clearance of DTPA in regions with normal V/Q ratios were compared with embolized regions with high V/Q ratios. Clearance rates were increased in embolized regions (V/Q ratio > 1) : 2.59 +- (SD) 0.89%. min-1, compared with normal regions (V/Q ratio -1 (p<0.01). In repeat studies in nine patients (one died), after intervals between 2 and 12 weeks, the V/Q ratio of previously embolized regions decreased towards unity in all nine patients, suggesting resolution. The differences in clearance rates for DTPA between normal and embolized regions decreased in association with this return towards normal of V/Q ratios. We surmise that reduction in pulmonary artery perfusion which occurs in pulmonary embolic disease alters the integrity of the alveolar (and possibly bronchiolar) epithelium. This effect is largely reversible, recovering with return of pulmonary artery perfusion

  1. [111In]-DTPA-D-phenylalanine-octreotide SPECT for scintigraphic imaging of an enhanced somatostatin receptor density in endocrine ophthalmopathy

    International Nuclear Information System (INIS)

    Recently, [111In]-DTPA-D-phenylalanine-octreotide was introduced for clinical use. This radioligand binds specifically to somatostatin-receptors and is suitable for SPECT examinations. The aim of this study was to clarify whether an increased somatostatin receptor density can be imaged and quantified in patients with endocrine ophthalmopathy (e.o.). 7 patients between 34 and 55 years with e.o. at stages III to VI and 4 controls between 38 and 63 years were examined. All patients and controls received approximately 200 MBq [111In]-DTPA-D-phenylalanine-octreotide by IV injection. A SPECT examination was performed 4 hours after injection and a normalised tracer uptake (An) was calculated for both orbitae. In partients with e.o. the values of An were significantly higher compared with controls (P=0.002). There was a correlation between An and exophthalmus stages according to Hertel with r=0.844 (P=0.001). These results indicate that [111In]-DTPA-D-phenylalanine-octreotide SPECT might be useful for the in vivo assessment of an increased somatostatin receptor density in e.o. These findings could have an impact on the treatment with somatostatin analogous in e.o. (orig.)

  2. Efeito da reação do solo no zinco extraído pelas soluções de dtpa e Mehlich-1 Soil reaction effect on dtpa and Mehlich-1 extractable zinc

    Directory of Open Access Journals (Sweden)

    Cleide Aparecida de Abreu

    1996-01-01

    Full Text Available A análise do solo é uma ferramenta eficiente para avaliar a disponibilidade de Zn, desde que seja escolhido adequadamente o processo de extração. Neste trabalho, desenvolvido em casa de vegetação, em Campinas, em 1993, avaliou-se a influência da calagem no Zn extraível do solo, usando-se o milho como planta-teste. Utilizaram-se amostras de cinco solos, sendo duas de latossolo vermelho-amarelo, uma de latossolo húmico, uma de latossolo roxo e uma de terra roxa estruturada, as quais receberam cinco doses de calcário. Antes e depois do cultivo do milho, retiraram-se amostras de solos para análise de Zn, usando-se os extratores DTPA e Mehlich-1. Na fase inicial, as plantas de milho apresentaram sintomas de deficiência de Zn, caracterizados por uma coloração amarelo-clara e altura reduzida. 0 extrator DTPA mostrou os melhores resultados com valores altos e positivos de correlação entre os teores de Zn na planta e no solo. Concluiu-se que a mudança na disponibilidade de Zn, decorrente da reação do solo, foi mais bem avaliada pela solução de DTPA.Soil testing can be adequate to evaluate the Zn availability if a good method is chosen. This experiment was conducted in a greenhouse, in Campinas, State of São Paulo, Brazil, in 1993, to evaluate the influence of liming on extractable Zn from soils, using corn as a test plant. Five rates of limestone were applied to four Oxisols and one Alfisol. Before and after the cultivation of corn, soil samples were taken and analyzed by extracting procedures of DTPA and Mehlich-1. Five days after emergence, corn seedlings showed leaf yellowing and reduced plant height (typical Zn deficiency symptoms, variable according to the liming level and soil studied. DTPA methodology showed the best results, with high and positive correlations estimated between plant-Zn and soil-Zn contents.

  3. Electrochemical and optical sugar sensors based on phenylboronic acid and its derivatives

    Energy Technology Data Exchange (ETDEWEB)

    Egawa, Yuya; Seki, Toshinobu [Faculty of Pharmaceutical Sciences, Josai University, Keyakidai, Sakado, Saitama 350-0295 (Japan); Takahashi, Shigehiro [Graduate School of Pharmaceutical Sciecnes, Tohoku University, Aramaki, Aoba-ku, Sendai 980-8578 (Japan); Anzai, Jun-ichi, E-mail: junanzai@mail.pharm.tohoku.ac.jp [Graduate School of Pharmaceutical Sciecnes, Tohoku University, Aramaki, Aoba-ku, Sendai 980-8578 (Japan)

    2011-10-10

    Recent progress in electrochemical and optical sugar sensors based on phenylboronic acid (PBA) and its derivatives as recognition components is reviewed. PBAs are known to bind diol compounds including sugars to form cyclic boronate esters that are negatively charged as a result of the addition of OH{sup -} ions from solution. Based on the formation of PBA charged species, sugars and their derivatives can be detected by means of electrochemical and optical techniques. For the development of PBA-based electrochemical sensing systems or sensors, PBA is modified with a redox-active marker, because PBA itself is electrochemically inactive, and ferrocene derivatives are often employed for this purpose. Ferrocene-modified PBAs have been used as redox-active additives in solution for the electrochemical detection of sugars and derivatives. PBA-modified electrodes have also been constructed as reagentless electrochemical sensors, where PBAs are immobilized on the surface of metal and carbon electrodes through mainly two routes: as a self-assembled monolayer film and as a polymer thin film. PBA-modified electrodes can be successfully used to detect sugars and derivatives through potentiometric and voltammetric responses. In addition, PBA-modified electrodes can be used for the immobilization of glycoenzymes on an electrode surface by the formation of boronate esters with carbohydrate chains in the glycoenzymes, thus resulting in enzyme biosensors. For the development of PBA-based optical sensors, a variety of chromophores and fluorophores have been coupled with PBA. Azobenzene dyes have been most frequently used for the preparation of colorimetric sugar sensors, in which the absorption wavelength and intensity of the dye are dependent on the type and concentration of added sugars. The sensitivity of the sensors is significantly improved based on multi-component systems in which alizalin red S, pyrocatechol violet, starch-iodine complex, and cyclodextrin are employed as

  4. Electrochemical and optical sugar sensors based on phenylboronic acid and its derivatives

    International Nuclear Information System (INIS)

    Recent progress in electrochemical and optical sugar sensors based on phenylboronic acid (PBA) and its derivatives as recognition components is reviewed. PBAs are known to bind diol compounds including sugars to form cyclic boronate esters that are negatively charged as a result of the addition of OH- ions from solution. Based on the formation of PBA charged species, sugars and their derivatives can be detected by means of electrochemical and optical techniques. For the development of PBA-based electrochemical sensing systems or sensors, PBA is modified with a redox-active marker, because PBA itself is electrochemically inactive, and ferrocene derivatives are often employed for this purpose. Ferrocene-modified PBAs have been used as redox-active additives in solution for the electrochemical detection of sugars and derivatives. PBA-modified electrodes have also been constructed as reagentless electrochemical sensors, where PBAs are immobilized on the surface of metal and carbon electrodes through mainly two routes: as a self-assembled monolayer film and as a polymer thin film. PBA-modified electrodes can be successfully used to detect sugars and derivatives through potentiometric and voltammetric responses. In addition, PBA-modified electrodes can be used for the immobilization of glycoenzymes on an electrode surface by the formation of boronate esters with carbohydrate chains in the glycoenzymes, thus resulting in enzyme biosensors. For the development of PBA-based optical sensors, a variety of chromophores and fluorophores have been coupled with PBA. Azobenzene dyes have been most frequently used for the preparation of colorimetric sugar sensors, in which the absorption wavelength and intensity of the dye are dependent on the type and concentration of added sugars. The sensitivity of the sensors is significantly improved based on multi-component systems in which alizalin red S, pyrocatechol violet, starch-iodine complex, and cyclodextrin are employed as indicators

  5. High efficiency, blue emitting materials based on phenanthro[9,10-d]imidazole derivatives

    Energy Technology Data Exchange (ETDEWEB)

    Jayabharathi, J., E-mail: jtchalam2005@yahoo.co.in; Sathishkumar, R.; Thanikachalam, V.; Jayamoorthy, K.

    2014-09-15

    The blue light emitting materials based on a fluoro phenanthro [9,10-d] imidazole derivatives prepared by a facial synthetic process exhibit good thermal stability, highly efficient fluorescence and balanced carrier injection. The multi-layered device based on fluoro phenanthroimidazole derivatives shows a higher luminance in a lower turn-on voltage. The device performance implies that the phenanthroimidazole unit is an excellent building block for tuning the carrier injection properties as well as blue emission. - Highlights: • Phenanthroimidazole exhibits high T{sub m} and T{sub d5} values, fluorescent efficiency, preparation cost and charge injection property. • The carrier injection and transport ability can be evidenced from the hole-only and electron-only devices. • These materials are used as building block for efficient blue light emitting materials. • Chemical structure modification improving the materials' properties.

  6. Excretion of purine base derivatives after intake of bacterial protein meal in pigs

    DEFF Research Database (Denmark)

    Hellwing, Anne Louise Frydendahl; Tauson, Anne-Helene; Skrede, A.

    2007-01-01

    Bacterial protein meal has a high content ofprotein but also of RNA and DNA. Sixteen barrows were allocated to four diets containing increasing levels of bacterial protein meal (BPM), from weaning to 80 kg live weight, to evaluate whether the RNA and DNA contents of BPM influenced the retention...... of nitrogen. It was hypothesised that an increased intake of RNA and DNA would lead to an increased urinary excretion of purine base derivatives and increased plasma concentrations. Retention of nitrogen was unaffected by dietary content of BPM (P=0.08) and the urinary excretion of purine base derivatives...... increased with increasing dietary content of BPM. No differences in fasting plasma concentration of uric acid, xanthine and hypoxanthine were observed. It can therefore be concluded that increasing levels of dietary BPM maintained protein accretion and led to changes in excretion of purine detrivatices...

  7. Interlaminar Stresses by Refined Beam Theories and the Sinc Method Based on Interpolation of Highest Derivative

    Science.gov (United States)

    Slemp, Wesley C. H.; Kapania, Rakesh K.; Tessler, Alexander

    2010-01-01

    Computation of interlaminar stresses from the higher-order shear and normal deformable beam theory and the refined zigzag theory was performed using the Sinc method based on Interpolation of Highest Derivative. The Sinc method based on Interpolation of Highest Derivative was proposed as an efficient method for determining through-the-thickness variations of interlaminar stresses from one- and two-dimensional analysis by integration of the equilibrium equations of three-dimensional elasticity. However, the use of traditional equivalent single layer theories often results in inaccuracies near the boundaries and when the lamina have extremely large differences in material properties. Interlaminar stresses in symmetric cross-ply laminated beams were obtained by solving the higher-order shear and normal deformable beam theory and the refined zigzag theory with the Sinc method based on Interpolation of Highest Derivative. Interlaminar stresses and bending stresses from the present approach were compared with a detailed finite element solution obtained by ABAQUS/Standard. The results illustrate the ease with which the Sinc method based on Interpolation of Highest Derivative can be used to obtain the through-the-thickness distributions of interlaminar stresses from the beam theories. Moreover, the results indicate that the refined zigzag theory is a substantial improvement over the Timoshenko beam theory due to the piecewise continuous displacement field which more accurately represents interlaminar discontinuities in the strain field. The higher-order shear and normal deformable beam theory more accurately captures the interlaminar stresses at the ends of the beam because it allows transverse normal strain. However, the continuous nature of the displacement field requires a large number of monomial terms before the interlaminar stresses are computed as accurately as the refined zigzag theory.

  8. Densities and Derived Thermodynamic Properties of Imidazolium-, Pyridinium-, Pyrrolidinium-, and Piperidinium-Based Ionic Liquids

    OpenAIRE

    Gardas, Ramesh L.; Costa, Henrique F.; Freire, Mara G.; Carvalho, Pedro J.; Marrucho, Isabel M.; Fonseca, Isabel M. A.; Ferreira, Abel G. M.; Coutinho, João A. P.

    2008-01-01

    In the present work, experimental density measurements are reported along with the derived thermodynamic properties, such as the isothermal compressibility (κT), the isobaric expansivity (αp), and the thermal pressure coefficient (γv) for imidazolium-, pyridinium-, pyrrolidinium-, and piperidinium-based ionic liquids (ILs), namely, 1-ethyl-3-methylimidazolium trifluoromethanesulfonate [C2mim][CF3SO3], 3-methyl-1-propylpyridinium bis(trifluoromethylsulfonyl)imide [C3mpy][NTf2], 1-methyl-1-prop...

  9. Synthesis, Characterization and Biological Evaluation of 1, 2-Disubstituted Benzimidazole Derivatives using Mannich Bases

    OpenAIRE

    Reddy, B. Anil

    2010-01-01

    The ring system in which a benzene ring is fused to the 4,5-positions of imidazole is designated as benzimidazole. Condensations of 2-substituted benzimidazole derivatives were synthesized by different carboxylic acids using Mannich base and anti-inflammatory activity. The various positions on the benzimidazole ring are numbered in the manner indicated with the imino function as number one. The formations of the product were conformed by the analytical and spectral data.

  10. Antibacterial Evaluation of Some Schiff Bases Derived from 2-Acetylpyridine and Their Metal Complexes

    OpenAIRE

    Thong Kwai Lin; Chai Lay Ching; Cher Lin Ooi; Hadi, A. Hamid A.; Mahmood Ameen Abdulla; Nura Suleiman Gwaram; Hapipah Mohd Ali; Hamid Khaledi

    2012-01-01

    A series of Schiff bases derived from 2-acetylpyridne and their metal complexes were characterized by elemental analysis, NMR, FT-IR and UV-Vis spectral studies. The complexes were screened for anti-bacterial activity against Methicillin-resistant Staphylococcus aureus (MRSA), Acinetobacter baumanni (AC), Klebsiella pneumonie (KB) and Pseudomonas aeruginosa (PA) using the disc diffusion and micro broth dilutio...

  11. Derivation of Myogenic Progenitors Directly From Human Pluripotent Stem Cells Using a Sphere-Based Culture

    OpenAIRE

    Hosoyama, Tohru; McGivern, Jered V.; Van Dyke, Jonathan M.; Allison D Ebert; Suzuki, Masatoshi

    2014-01-01

    The authors present a novel protocol for deriving myogenic progenitors from human embryonic stem cells and induced pluripotent stem cells using free-floating spherical culture. Results show that sphere-based cultures of human pluripotent stem cells, expanded in medium containing high concentrations of fibroblast growth factor and epidermal growth factor, can propagate myogenic progenitors from human embryonic stem cells and healthy and disease-specific induced pluripotent stem cells.

  12. Human Bone Marrow-derived Mesenchymal Stem Cell: A Source for Cell-Based Therapy

    OpenAIRE

    Ayatollahi, M.; Geramizadeh, B; Zakerinia, M; M Ramzi; Yaghobi, R.; Hadadi, P.; Rezvani, A. R.; Aghdai, M.; N Azarpira; Karimi, H.

    2012-01-01

    Background: The ability of mesenchymal stem cells (MSCs) to differentiate into many cell types, and modulate immune responses, makes them an attractive therapeutic tool for cell transplantation and tissue engineering. Objective: This project was designed for isolation, culture, and characterization of human marrow-derived MSCs based on the immunophenotypic markers and the differentiation potential. Methods: Bone marrow of healthy donors was aspirated from the iliac crest. Mononuclear cells we...

  13. Microcalcifications clinically normal breast: the value of high field, surface coil, Gd-DTPA-enhanced MRI

    Energy Technology Data Exchange (ETDEWEB)

    Tesoro-Tess, J.D. [Diagnostic Radiology E, Istituto Nazionale Tumori, Milan (Italy); Amoruso, A. [Diagnostic Radiology E, Istituto Nazionale Tumori, Milan (Italy); Rovini, D. [Surgical Div. C, Istituto Nazionale Tumori, Milan (Italy); Balzarini, L. [Diagnostic Radiology E, Istituto Nazionale Tumori, Milan (Italy); Ceglia, E. [Diagnostic Radiology E, Istituto Nazionale Tumori, Milan (Italy); Trecate, G. [Diagnostic Radiology E, Istituto Nazionale Tumori, Milan (Italy); Savio, T. [Surgical Div. C, Istituto Nazionale Tumori, Milan (Italy); Musumeci, R. [Diagnostic Radiology E, Istituto Nazionale Tumori, Milan (Italy); Civelli, E. [Diagnostic Radiology E, Istituto Nazionale Tumori, Milan (Italy)

    1995-12-31

    State-of-the-art screening mammography allows the detection of nonpalpable breast lesions in approximately 30% of patients. The presence of clustered microcalcifications without evidence of solid tumors usually requires further investigations, mainly biopsy. A 1.5-T magnet with a single breast coil was used to evaluate 32 patients with indeterminate mammography suggestive of microcalcifications prior to surgery. Both spin-echo (SE) and gradient-echo (GE; 2D fast low-angle shot [FLASH]) techniques were utilized before and after injection of 0.2 mmol/kg Gd-DTPA. Upon surgery tumor diameters ranged between 3 and 10 mm. Use of MRI demonstrated 87.5% overall accuracy, 83.3% sensitivity, and 92.9% specificity. False-negative MRI results were in situ carcinomas less than 5 mm in size. All the correctly diagnosed carcinomas measured between 5 and 10 mm. Partial volume is probably the greatest limit of this technique and lesions equal to or smaller than 5 mm are only rarely detected. The GE and SE sequences demonstrated comparable results. (orig.)

  14. Gd-DTPA-enhanced Three-dimensional MR imaging of degenerative disease of the cervical spine

    International Nuclear Information System (INIS)

    This paper assesses cervical spine three-dimensional (3D) MR imaging with Gd-DTPA, with the hypothesis that an enhanced 3D sequence with reconstructions would suffice for the evaluation of degenerative disease. Sixty patients were examined with (1) T1-weighted spin-echo and axial 2D gradient-echo low-flip-angle images representing the standard examination; and (2) one of two different enhanced 3D gradient-echo techniques: 30 patients with 3D fast low-angle shot (FLASH) imaging (TR 40/TE 7, 40 degrees flip angle, 64 2-mm sagittal partitions, one excitation, 10:67 minutes) and 30 with 3D Turbo FLASH imaging (MP RAGE) (7/5, 10 degrees, 128 1.5-mm partitions, one excitation, 6:07 minutes). 3D studies were reconstructed at 45 degrees obliquities for foramina, and axially. Standard and 3D studies were evaluated independently by two neuroradiologists for the location and size of extradural disease

  15. Preparation and quality control of {sup 166}Ho-DTPA-antiCD20 for radioimmunotherapy

    Energy Technology Data Exchange (ETDEWEB)

    Zolghadri, S.; Jalilian, A.R.; Yousefnia, H.; Bahrami-Sumani, A.; Shirvani-Arani, S.; Ghannadi-Maragheh, M. [Nuclear Science and Technology Research Institute (NSTRI), Tehran (IR). Radiopharmaceutical Research and Development Lab. (RRDL)

    2011-07-01

    In this work, anti-CD20 was successively labeled with beta-particle emitting radionuclide, Ho-166, for ultimate radioimmunotherapy applications. Ho-166 chloride was obtained by thermal neutron flux (1 x 10{sup 13} n cm{sup -2} s{sup -1}) of natural Ho{sub 2}(NO{sub 3}){sub 3} sample, dissolved in acidic media. {sup 166}Ho-holmium chloride (185 MBq) was added to the conjugated antibody after ccDTPA residulation at room temperature. Radiochemical purity of 95% (ITLC) and 98% (HPLC) were obtained for final radioimmunoconjugate (specific activity = 3-3.5 GBq/mg). The final isotonic {sup 166}Ho-rituximab complex was checked by gel electrophoresis for protein integrity retention. Biodistribution studies of Ho-166 chloride and radioimmunoconjugate were performed in wild-type rats to determine the biodistribution. The accumulation of the radiolabeled antibody in lungs, liver and spleen demonstrates a similar pattern to the other radiolabeled anti-CD20 immunoconjugates. (orig.)

  16. General hybrid projective complete dislocated synchronization with non-derivative and derivative coupling based on parameter identification in several chaotic and hyperchaotic systems

    Institute of Scientific and Technical Information of China (English)

    Sun Jun-Wei; Shen Yi; Zhang Guo-Dong; Wang Yan-Feng; Cui Guang-Zhao

    2013-01-01

    According to the Lyapunov stability theorem,a new general hybrid projective complete dislocated synchronization scheme with non-derivative and derivative coupling based on parameter identification is proposed under the framework of drive-response systems.Every state variable of the response system equals the summation of the hybrid drive systems in the previous hybrid synchronization.However,every state variable of the drive system equals the summation of the hybrid response systems while evolving with time in our method.Complete synchronization,hybrid dislocated synchronization,projective synchronization,non-derivative and derivative coupling,and parameter identification are included as its special item.The Lorenz chaotic system,Rossler chaotic system,memristor chaotic oscillator system,and hyperchaotic Lii system are discussed to show the effectiveness of the proposed methods.

  17. Synthesis, characterization, and evaluation of 10-undecenoic acid-based epithio derivatives as multifunctional additives.

    Science.gov (United States)

    Geethanjali, Gorla; Padmaja, Korlipara V; Sammaiah, Arukali; Prasad, Rachapudi B N

    2014-11-26

    Novel epithio compounds from alkyl epoxy undecanoates (n-alkyl, C1, C4, and C6; isoalkyl, C3, C4, and C8) were synthesized using an ammonium thiocyanate in ionic liquid 1-methylimidazolium tetrafluoroborate/H2O (2:1) solvent system in 85-90% yields by gas chromatographic (GC) analysis. The synthesized products were characterized by (1)H and (13)C nuclear magnetic resonance spectroscopy, Fourier transform infrared spectroscopy (FTIR), gas chromatography, and GC mass spectral (GC-MS) analyses and evaluated for their antioxidant, extreme pressure (EP), and antiwear (AW) properties in three different base oils, namely, epoxy jatropha fatty acid n-butyl esters (EJB), di-2-ethylhexyl sebacate (DOS), and mineral oil (S-105). Among the synthesized products, n-butyl epithio undecanoate exhibited superior antioxidant property (229.2 °C) compared to butylated hydroxytoluene (BHT, 193.8 °C) in base oil DOS and comparable performance in EJB and S-105 base oils. All of the epithio derivatives exhibited significantly enhanced weld point for the base oils EJB and DOS at 2 wt % level and displayed moderate enhancement in S-105 base oil. Methyl epithio undecanoate at 0.6% concentration exhibited considerable improvement in the wear scar of DOS base oil. The synthesized epithio derivatives have potential as multifunctional additives in lubricant formulations. PMID:25369173

  18. MR imaging of the biliary tract with Gd-EOB-DTPA: Effect of liver function on signal intensity

    Energy Technology Data Exchange (ETDEWEB)

    Takao, Hidemasa, E-mail: takaoh-tky@umin.ac.jp [Department of Radiology, Graduate School of Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655 (Japan); Akai, Hiroyuki; Tajima, Taku [Department of Radiology, Graduate School of Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655 (Japan); Kiryu, Shigeru [Department of Radiology, Institute of Medical Science, University of Tokyo, 74-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639 (Japan); Watanabe, Yasushi [Department of Radiological Technology, The University of Tokyo Hospital, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655 (Japan); Imamura, Hiroshi [Department of Hepatobiliary-Pancreatic Surgery, Juntendo University School of Medicine, 3-1-3 Hongo, Bunkyo-ku, Tokyo 113-8431 (Japan); Akahane, Masaaki; Yoshioka, Naoki [Department of Radiology, Graduate School of Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655 (Japan); Kokudo, Norihiro [Hepato-Biliary-Pancreatic Surgery Division, Department of Surgery, Graduate School of Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655 (Japan); Ohtomo, Kuni [Department of Radiology, Graduate School of Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655 (Japan)

    2011-02-15

    Objective: To quantitatively evaluate the signal intensity of the biliary tract in gadolinium ethoxybenzyl diethylenetriamine pentaacetic acid (Gd-EOB-DTPA)-enhanced magnetic resonance (MR) imaging and to investigate the effect of liver function on the signal intensity of the biliary tract. Materials and methods: A total of 32 patients with and without chronic liver disease (normal liver group, n = 15; chronic liver disease group, n = 17) were included in this study. All patients were prospectively enrolled for evaluation of known or suspected focal liver lesions. In the chronic liver disease group, the etiologies were chronic hepatitis C virus infection (n = 12) and chronic hepatitis B virus infection (n = 5). The median Child-Pugh score was 5 (range, 5-7). Each patient received the standard dose of Gd-EOB-DTPA (0.025 mmol/kg of body weight). Post-contrast T1-weighted MR images were obtained at 5, 10, 15, 20, 25, and 30 min after administration of Gd-EOB-DTPA. Maximum signal intensities (SIs) of the right and left hepatic ducts, common hepatic duct, and common bile duct were measured. Relative signal intensity was calculated as follows: relative SI = maximum SI{sub bileduct}/mean SI{sub muscle}. Serum albumin level, serum total bilirubin level, prothrombin time, indocyanine green retention rate at 15 min (ICG-R15), and estimated glomerular filtration rate were entered into regression analysis. Results: The signal intensity of the bile duct reached a peak 30 min after administration of Gd-EOB-DTPA. The mean relative signal intensity of the right and left hepatic ducts at the peak time point was not significantly different between the two groups, while increase in signal intensity was delayed in the chronic liver disease group. The mean relative signal intensity of the common hepatic duct and that of the common bile duct at the peak time point were significantly different between the two groups (Wilcoxon rank-sum test, P = 0.03, respectively). Stepwise regression

  19. Synthesis of Some Novel Heterocyclic and Schiff Base Derivatives as Antimicrobial Agents

    Directory of Open Access Journals (Sweden)

    Mohamed E. Azab

    2015-10-01

    Full Text Available Treatment of 2,3-diaryloxirane-2,3-dicarbonitriles 1a–c with different nitrogen nucleophiles, e.g., hydrazine, methyl hydrazine, phenyl hydrazine, hydroxylamine, thiosemicarbazide, and/or 2-amino-5-phenyl-1,3,4-thiadiazole, afforded pyrazole, isoxazole, pyrrolotriazine, imidazolothiadiazole derivatives 2–5, respectively. Reacting pyrazoles 2a–c with aromatic aldehydes and/or methyl glycinate produced Schiff’s bases 7a–d and pyrazolo[3,4-b]-pyrazinone derivative 8, respectively. Treating 7 with ammonium acetate and/or hydrazine hydrate, furnished the imidazolopyrazole and pyrazolotriazine derivatives 9 and 10, respectively. Reaction of 8 with chloroacetic acid and/or diethyl malonate gave tricyclic compound 11 and triketone 12, respectively. On the other hand, compound 1 was reacted with active methylene precursors, e.g., acetylacetone and/or cyclopentanone producing adducts 14a,b which upon fusion with ammonium acetate furnished the 3-pyridone derivatives 15a,b, respectively. Some of newly synthesized compounds were screened for activity against bacterial and fungal strains and most of the newly synthesized compounds showed high antimicrobial activities. The structures of the new compounds were elucidated using IR, 1H-NMR, 13C-NMR and mass spectroscopy.

  20. Site-specific water quality guidelines: 1. Derivation approaches based on physicochemical, ecotoxicological and ecological data.

    Science.gov (United States)

    van Dam, R A; Humphrey, C L; Harford, A J; Sinclair, A; Jones, D R; Davies, S; Storey, A W

    2014-01-01

    Generic water quality guidelines (WQGs) are developed by countries/regions as broad scale tools to assist with the protection of aquatic ecosystems from the impacts of toxicants. However, since generic WQGs cannot adequately account for the many environmental factors that may affect toxicity at a particular site, site-specific WQGs are often needed, especially for high environmental value ecosystems. The Australian and New Zealand Guidelines for Fresh and Marine Water Quality provide comprehensive guidance on methods for refining or deriving WQGs for site-specific purposes. This paper describes three such methods for deriving site-specific WQGs, namely: (1) using local reference water quality data, (2) using biological effects data from laboratory-based toxicity testing, and (3) using biological effects data from field surveys. Two case studies related to the assessment of impacts arising from mining operations in northern Australia are used to illustrate the application of these methods. Finally, the potential of several emerging methods designed to assess thresholds of ecological change from field data for deriving site-specific WQGs is discussed. Ideally, multiple lines of evidence approaches, integrating both laboratory and field data, are recommended for deriving site-specific WQGs. PMID:23846952

  1. Antibacterial evaluation of some Schiff bases derived from 2-acetylpyridine and their metal complexes.

    Science.gov (United States)

    Gwaram, Nura Suleiman; Ali, Hapipah Mohd; Khaledi, Hamid; Abdulla, Mahmood Ameen; Hadi, A Hamid A; Lin, Thong Kwai; Ching, Chai Lay; Ooi, Cher Lin

    2012-01-01

    A series of Schiff bases derived from 2-acetylpyridne and their metal complexes were characterized by elemental analysis, NMR, FT-IR and UV-Vis spectral studies. The complexes were screened for anti-bacterial activity against Methicillin-resistant Staphylococcus aureus (MRSA), Acinetobacter baumanni (AC), Klebsiella pneumonie (KB) and Pseudomonas aeruginosa (PA) using the disc diffusion and micro broth dilution assays. Based on the overall results, the complexes showed the highest activities against MRSA while a weak antibacterial activity was observed against A. baumanii and P. aeruginosa. PMID:22609786

  2. Synthesis of vegetable oil based polyol with cottonseed oil and sorbitol derived from natural source

    Institute of Scientific and Technical Information of China (English)

    Lian Kun Jia; Li Xiang Gong; Wen Jiao Ji; Cheng You Kan

    2011-01-01

    In order to prepare the polyol with all bio-based components as raw materials, cottonseed oil was first epoxidized by peroxyformic acid generated in situ from hydrogen peroxide and formic acid, and the cottonseed oil based polyols with variable hydroxyl value were then prepared by the ring-opening of epoxidized cottonseed oil with sorbitol, which is a multi-functional hydroxyl compound derived from a natural source. The chemical structure of the products was characterized with FTIR analysis, and the residual epoxy oxygen content and hydroxyl value of the polyol versus the ring-opening time were investigated.

  3. Pulmonary functional MRI:an animal model study of oxygen-enhanced ventilation combined with Gd-DTPA-enhanced perfusion

    Institute of Scientific and Technical Information of China (English)

    杨健; 万明习; 郭佑民

    2004-01-01

    Background The assessment of regional pulmonary ventilation and perfusion is essential for the evaluation of a variety of lung disorders. Pulmonary ventilation MRI using inhaled oxygen as a contrast medium can be obtained with a clinical MR scanner, without additional equipment, and has been demonstrated to be a feasible means of assessing ventilation in animal models and some clinical patients. However, few studies have reported on MR ventilation-perfusion imaging. In this study, we evaluated the usefulness of oxygen-enhanced ventilation in combination with first-pass Gd-DTPA-enhanced perfusion MRI in a canine model of pulmonary embolism and airway obstruction.Methods Peripheral pulmonary embolisms were produced in eight dogs by intravenous injection of gelfoam strips at the pulmonary segmental arterial level, and airway obstructions were created in five of the dogs by inserting a self-designed balloon catheter into a secondary bronchus. Oxygen-enhanced MR ventilation images were produced by subtracting images from before and after inhalation of pure oxygen. Pulmonary perfusion MR images were acquired with a dynamic three-dimensional fast gradient-echo sequence. MR ventilation and perfusion images were read and contrasted with results from general examinations of pathological anatomy, ventilation-perfusion scintigraphy, and pulmonary angiography. Results Regions identified as having airway obstructions matched using both MR ventilation and perfusion imaging, but regions of pulmonary embolisms were mismatched. The area of airway obstruction defects was smaller using MR ventilation imagery than that using ventilation scintigraphy. Abnormal perfusion regions due to pulmonary embolisms were divided into defective regions and reduced regions based on the time course of signal intensity changes. In the diagnosis of pulmonary embolisms with the technique of ventilation and perfusion MRI, sensitivity and specificity were 75.0% and 98.1%, respectively, and the diagnostic

  4. Clearance of technetium-99m-labeled DTPA in hyperthyroidism without clinical evidence of lung disease, and relation to pulmonary function

    International Nuclear Information System (INIS)

    The mechanisms of dyspnea and exercise intolerance have not been fully elucidated. We aimed to investigate the clearance rate of technetium-99m diethyltriaminepentaaceticacid (Tc-99m DTPA) from lungs in hyperthyroid patients without clinical evidence of lung disease and to explore the interactions between their Tc-99m DTPA radioaerosol lung scintigraphy, spirometric measurements, and the levels of thyroid hormones. We studied 19 hyperthyroid patients and 16 sex- and age-matched controls. Thyroid hormone levels were assessed. Spirometric lung function tests, diffusing capacity of the lung for carbon monoxide (DLCO) and the clearance rate of Tc-99m DTPA were performed in all participants. Ratio of DLCO value to the alveolar ventilation (DLCO/VA) and the means of half-time (T1/2) of Tc-99m DTPA clearance rate, which were used to evaluate alveolar-capillary membrane permeability, were calculated. There were no statistical differences between spirometric parameters (vital capacity (VC), force vital capacity (FVC), one second forced expiratory volume (FEV1)/FVC, mean forced expiratory flow during the middle of FVC (FEF 25-75)) of the two groups (p>0.05). Although the mean FEV1 level was significantly lower in the hyperthyroid patients than the control subjects (p1 was only less than 80 percent of the predicted value. No significant difference in the means of DLCO, DLCO/VA or T1/2 values of Tc-99m DTPA clearance was observed between the two groups (p>0.05). In hyperthyroid patients, there was a positive relation between DLCO/VA, DLCO/VA% and T1/2 values of Tc-99m DTPA clearance (p1/2 values of Tc-99m DTPA clearance in hyperthyroid group (p>0.05). We conclude that increased thyroid hormones have no effect on permeability of alveolar-capillary membrane in hyperthyroid patients. (author)

  5. Lightweight and Distributed Connectivity-Based Clustering Derived from Schelling's Model

    Science.gov (United States)

    Tsugawa, Sho; Ohsaki, Hiroyuki; Imase, Makoto

    In the literature, two connectivity-based distributed clustering schemes exist: CDC (Connectivity-based Distributed node Clustering scheme) and SDC (SCM-based Distributed Clustering). While CDC and SDC have mechanisms for maintaining clusters against nodes joining and leaving, neither method assumes that frequent changes occur in the network topology. In this paper, we propose a lightweight distributed clustering method that we term SBDC (Schelling-Based Distributed Clustering) since this scheme is derived from Schelling's model — a popular segregation model in sociology. We evaluate the effectiveness of the proposed SBDC in an environment where frequent changes arise in the network topology. Our simulation results show that SBDC outperforms CDC and SDC under frequent changes in network topology caused by high node mobility.

  6. Single-phase Phase-locked Loop Based on Derivative Elements

    DEFF Research Database (Denmark)

    Guan, Qingxin; Zhang, Yu; Kang, Yong;

    2016-01-01

    High performance phase locked loops (PLLs) are critical for power control in grid-connected systems. This paper presents a new method of designing a PLL for single-phase systems based on derivative elements (DEs). The quadrature signal generator (QSG) is constructed by two DEs with the same main...... parameters. The PLL itself is realized by using the DE-based QSG. It avoids errors due to the overlap and accumulation that are present in PLLs based on integral elements, such as a PLL based on a second-order generalized integrator (SOGI-PLL). Additionally, frequency feedback is not needed which allows...... the proposed PLL to achieve high performance when the grid frequency changes rapidly. This paper presents the model of the PLL and a theoretical performance analysis with respect to both the frequency-domain and time-domain behavior. The error arising from the discretization process is also compensated...

  7. Induced cholesteric systems based on some cyano derivatives as host phases

    Science.gov (United States)

    Shkolnikova, Natalya I.; Kutulya, Lidiya A.; Vashchenko, V. V.; Fedoryako, A. P.; Lapanik, V. I.; Posledovich, N. R.

    2002-12-01

    Macroscopical properties of some induced cholesteric compositions based on 4-pentyl-4'-cyano derivatives of biphenyl and phenylcyclohexane as host phases have been investigated. The series of N-arylidene derivatives of (S)-1-phenylethylamine with varied both rigid moiety of the N-arylidene fragment and terminal substituent was used as chiral dopants. The influence of the chiral dopant molecular structure as well as of physical properties of the host phases used on the helical twisting power, the temperature dependence of the induced helical pitch and the N* mesophase thermal stability has been characterized. It has been concluded that the distinctions in properties of the LC systems containing the OCH2 and COO linking groups are caused by their different conformational states.

  8. Fingerprint Image Enhancement Based on Second Directional Derivative of the Digital Image

    Directory of Open Access Journals (Sweden)

    Onnia Vesa

    2002-01-01

    Full Text Available This paper presents a novel approach of fingerprint image enhancement that relies on detecting the fingerprint ridges as image regions where the second directional derivative of the digital image is positive. A facet model is used in order to approximate the derivatives at each image pixel based on the intensity values of pixels located in a certain neighborhood. We note that the size of this neighborhood has a critical role in achieving accurate enhancement results. Using neighborhoods of various sizes, the proposed algorithm determines several candidate binary representations of the input fingerprint pattern. Subsequently, an output binary ridge-map image is created by selecting image zones, from the available binary image candidates, according to a MAP selection rule. Two public domain collections of fingerprint images are used in order to objectively assess the performance of the proposed fingerprint image enhancement approach.

  9. Origami mechanical metamaterials based on the Miura-derivative fold patterns

    Science.gov (United States)

    Zhou, Xiang; Zang, Shixi; You, Zhong

    2016-07-01

    This paper presents two new types of origami-inspired mechanical metamaterials based on the Miura-derivative fold patterns that consist of non-identical parallelogram facets. The analytical models to predict dimension changes and deformation kinematics of the proposed metamaterials are developed. Furthermore, by modelling the creases as revolute hinges with certain rotational spring constants, we derived analytical models for stretching and bulk moduli. The analytical models are validated through finite-element simulation results. Numerical examples reveal that the proposed metamaterials possess some intriguing properties, including negative Poisson's ratios and bulk modulus. The work presented in this paper can provide a highly flexible framework for the design of versatile tunable mechanical metamaterials.

  10. Phytoceramide and sphingoid bases derived from brewer's yeast Saccharomyces pastorianus activate peroxisome proliferator-activated receptors

    Directory of Open Access Journals (Sweden)

    Mitsutake Susumu

    2011-08-01

    Full Text Available Abstract Background Peroxisome proliferator-activated receptors (PPARs are ligand-activated transcription factors that regulate lipid and glucose metabolism. PPARα is highly expressed in the liver and controls genes involved in lipid catabolism. We previously reported that synthetic sphingolipid analogs, part of which contains shorter-length fatty acid chains than natural sphingolipids, stimulated the transcriptional activities of PPARs. Sphingosine and dihydrosphingosine (DHS are abundant sphingoid bases, and ceramide and dihydroceramide are major ceramide species in mammals. In contrast, phytosphingosine (PHS and DHS are the main sphingoid bases in fungi. PHS and phytoceramide exist in particular tissues such as the epidermis in mammals, and involvement of ceramide species in PPARβ activation in cultured keratinocytes has been reported. The purpose of the present study is to investigate whether natural sphingolipids with C18 fatty acid and yeast-derived sphingoid bases activate PPARs as PPAR agonists. Method Lipids of brewer's yeast contain PHS- and DHS-based sphingolipids. To obtain the sphingoid bases, lipids were extracted from brewer's yeast and acid-hydrolyzed. The sphingoid base fraction was purified and quantified. To assess the effects of sphingolipids on PPAR activation, luciferase reporter assay was carried out. NIH/3T3 and human hepatoma (HepG2 cells were transfected with expression vectors for PPARs and retinoid × receptors, and PPAR responsive element reporter vector. When indicated, the PPAR/Gal4 chimera system was performed to enhance the credibility of experiments. Sphingolipids were added to the cells and the dual luciferase reporter assay was performed to determine the transcriptional activity of PPARs. Results We observed that phytoceramide increased the transcriptional activities of PPARs significantly, whereas ceramide and dihydroceramide did not change PPAR activities. Phytoceramide also increased transactivation of

  11. Renogram studies with dogs, using the radioisotope technetium-99m DTPA

    International Nuclear Information System (INIS)

    In the dog extensive kidney pathology is often not diagnosed by conventional tests. In this study renograms were conducted on conscious healthy Beagle dogs using the radioisotope technetium-99m DTPA. Because of problems foreseen in keeping dogs immobile the study was also conducted using the sedative acepromazine maleate and the narcotic thiopentonesodium on the same Beagles. Renograms were also conducted on dogs with acute and chronic renal pathology. It was found that during the renogram procedure the animal must be completely immobile. Constant infusion narcosis with thiopentonesodium produced this immobility without affecting the renogram in normal dogs, however administration of a thiopentone bolus did affect the renogram. Sedation with acepromazine maleate had the effect of significantly increasing the second phase of the renogram and in addition significantly accelerated the excretion phase. These effects are thought to be due to a decreased bloodpressure with concomintent renal bloodpooling and retarded renal bloodflow. It would appear that radioisotope renograms are valuable in the diagnosis and prognosis of clinical cases of acute as well as chronic renal pathology. It is especially useful in identifying cases of compensated renal pathology where it is more sensitive than the conventional tests. It can differentiate between different degrees of pathology between the left and right kidney. Seen from an economic viewpoint the conventional tests give adequate information in the case of acute renal failure. Radioisotope renogram appears to hold great promise for both clinical and research applications. The equipment required for this application, however, is so costly that it would only be financially feasible for major centres such as a Faculty of Veterinary Science. Much information can be gained from referred clinical cases

  12. Water-pipe smoking effects on pulmonary permeability using technetium-99m DTPA inhalation scintigraphy

    International Nuclear Information System (INIS)

    Although extensive work has been done on cigarette smoking and its effects on pulmonary function, there are limited number of studies on water-pipe smoking. The effects of water-pipe smoking on health are not widely investigated. The aim of this study was to determine the effects of water-pipe smoking on pulmonary permeability. Technetium-99m DTPA inhalation scintigraphy was performed on 14 water-pipe smoker volunteers (all men, mean age 53.7±9.8) and 11 passive smoker volunteers (1 woman, 10 men, mean age 43.8±12). Clearance half-time (T 1/2) was calculated by placing a monoexponential fit on the time activity curves. Penetration index (PI) of the radioaerosol was also calculated. PI was 0.58±0.14 and 0.50±0.12 for water-pipe smokers (WPS) and passive smokers (PS) respectively. T 1/2 of peripheral lung was 57.3±12.7 and 64.6±13.2 min, central airways was 55.8±23.5 and 80.1±35.2 min for WPS and PS, respectively (p≤0.05). Forced expiratory volume in one second/forced vital capacity (FEV1/FVC)% was 82.1±8.5 (%) and 87.7±6.5 (%) for WPS and PS, respectively (0.025< p≤0.05). We suggest that water-pipe smoking effects pulmonary epithelial permeability more than passive smoking. Increased central mucociliary clearance in water-pipe smoking may be due to preserved humidity of the airway tracts. (author)

  13. Derivative Trade Optimizing Model Utilizing GP Based on Behavioral Finance Theory

    Science.gov (United States)

    Matsumura, Koki; Kawamoto, Masaru

    This paper proposed a new technique which makes the strategy trees for the derivative (option) trading investment decision based on the behavioral finance theory and optimizes it using evolutionary computation, in order to achieve high profitability. The strategy tree uses a technical analysis based on a statistical, experienced technique for the investment decision. The trading model is represented by various technical indexes, and the strategy tree is optimized by the genetic programming(GP) which is one of the evolutionary computations. Moreover, this paper proposed a method using the prospect theory based on the behavioral finance theory to set psychological bias for profit and deficit and attempted to select the appropriate strike price of option for the higher investment efficiency. As a result, this technique produced a good result and found the effectiveness of this trading model by the optimized dealings strategy.

  14. SYNTHESIS AND DOCKING STUDIES OF SCHIFF BASES DERIVED FROM 4-AMINOPYRIDINE

    Directory of Open Access Journals (Sweden)

    Jayita Nandi

    2012-10-01

    Full Text Available A Schiff base is a compound with a functional group that contains a carbon-nitrogen double bond with the nitrogen atom connected to aryl or alkyl group. In the present study, schiff bases were synthesized using 4-amino pyridine and different aldehydes, after assessing the drug likeliness properties. The structure of synthesised compounds were characterized by IR, NMR and Mass spectroscopic techniques. Docking studies were carried out for the schiff bases derived from 4-amino pyridine using Molegro Virtual Docker and protein beta-ketoacyl acyl carrier protein synthase II (mtKasB enzyme was selected as the target for M. tuberculosis. The results of docking study was compared with the standard drug isoniazid and the significant results were obtained.

  15. A kit to prepare {sup 111}In-DTPA-trastuzumab (Herceptin) Fab fragments injection under GMP conditions for imaging or radioimmunoguided surgery of HER2-positive breast cancer

    Energy Technology Data Exchange (ETDEWEB)

    Scollard, Deborah A.; Chan, Conrad [Department of Pharmaceutical Sciences, University of Toronto, Toronto, ON, M5S 3M2 (Canada); Holloway, Claire M.B. [Department of Surgery, Sunnybrook Health Sciences Centre, Toronto, ON, M4N 1H1 (Canada); Reilly, Raymond M., E-mail: raymond.reilly@utoronto.c [Department of Pharmaceutical Sciences, University of Toronto, Toronto, ON, M5S 3M2 (Canada); Department of Medical Imaging, University of Toronto, Toronto, ON, M5S 3E2 (Canada); Toronto General Research Institute, University Health Network, Toronto, ON, M5G 2M9 (Canada)

    2011-01-15

    Introduction: The human epidermal growth factor receptor-2 (HER2) gene is amplified in 25% of invasive breast cancers, and receptor overexpression has been noted in up to 60% of early stages of the disease [ductal carcinoma in situ (DCIS)]. Preclinical studies have revealed high tumor/blood ratios (>27:1) for {sup 111}In-labeled Fab fragments of the HER2 monoclonal antibody, trastuzumab (Herceptin) ({sup 111}In-DTPA-trastuzumab Fab) at 72 h pi in athymic mice bearing subcutaneous human breast cancer xenografts. Our aim in this study was to formulate a kit for preparation of {sup 111}In-DTPA-trastuzumab Fab injection under good manufacturing practice (GMP) conditions suitable for human administration in a Phase I clinical trial of imaging and radioimmunoguided surgery (RIGS) of HER2-positive breast cancer. Methods: Fab fragments were produced by digestion of trastuzumab IgG (Herceptin) with immobilized papain for 20 h at 37{sup o}C. Fab fragments were purified by ultrafiltration, then reacted with a 10-fold molar excess of diethylenetriaminepentaacetic acid (DTPA) dianhydride. DTPA-Fab fragments were purified, then sterilized by filtration into unit dose glass vials (kits). Kits were tested against specifications for volume (0.9-1.1 ml), protein concentration (0.45-0.55 mg/ml), pH (5.5-6.5), DTPA substitution (0.5-4.0 mol DTPA/mol Fab), appearance (clear, colorless and particle free), labeling efficiency ({>=}85%), and sterility and apyrogenicity (USP XXXII). Immunoreactivity of {sup 111}In-DTPA-trastuzumab Fab towards HER2 was measured by saturation radioligand binding assays using SKBR-3 human breast cancer cells (specifications: K{sub a}=0.6-9.6x10{sup 7} L/mol; B{sub max}=0.6-10.4x10{sup 6} sites/cell). {sup 111}In-DTPA-trastuzumab Fab injection was prepared by adding 80-100 MBq of {sup 111}InCl{sub 3} to a single kit vial and incubating for 30 min at room temperature. {sup 111}In-DTPA-trastuzumab Fab was assayed for the amount of radioactivity and tested for p

  16. Treatment of peritoneal carcinomatosis by targeted delivery of the radio-labeled tumor homing peptide bi-DTPA-[F3]2 into the nucleus of tumor cells.

    Directory of Open Access Journals (Sweden)

    Enken Drecoll

    Full Text Available BACKGROUND: Alpha-particle emitting isotopes are effective novel tools in cancer therapy, but targeted delivery into tumors is a prerequisite of their application to avoid toxic side effects. Peritoneal carcinomatosis is a widespread dissemination of tumors throughout the peritoneal cavity. As peritoneal carcinomatosis is fatal in most cases, novel therapies are needed. F3 is a tumor homing peptide which is internalized into the nucleus of tumor cells upon binding to nucleolin on the cell surface. Therefore, F3 may be an appropriate carrier for alpha-particle emitting isotopes facilitating selective tumor therapies. PRINCIPAL FINDINGS: A dimer of the vascular tumor homing peptide F3 was chemically coupled to the alpha-emitter (213Bi ((213Bi-DTPA-[F3](2. We found (213Bi-DTPA-[F3](2 to accumulate in the nucleus of tumor cells in vitro and in intraperitoneally growing tumors in vivo. To study the anti-tumor activity of (213Bi-DTPA-[F3](2 we treated mice bearing intraperitoneally growing xenograft tumors with (213Bi-DTPA-[F3](2. In a tumor prevention study between the days 4-14 after inoculation of tumor cells 6x1.85 MBq (50 microCi of (213Bi-DTPA-[F3](2 were injected. In a tumor reduction study between the days 16-26 after inoculation of tumor cells 6x1.85 MBq of (213Bi-DTPA-[F3](2 were injected. The survival time of the animals was increased from 51 to 93.5 days in the prevention study and from 57 days to 78 days in the tumor reduction study. No toxicity of the treatment was observed. In bio-distribution studies we found (213Bi-DTPA-[F3](2 to accumulate in tumors but only low activities were found in control organs except for the kidneys, where (213Bi-DTPA-[F3](2 is found due to renal excretion. CONCLUSIONS/SIGNIFICANCE: In conclusion we report that (213Bi-DTPA-[F3](2 is a novel tool for the targeted delivery of alpha-emitters into the nucleus of tumor cells that effectively controls peritoneal carcinomatosis in preclinical models and may also be

  17. Reduction of skeletal accumulation of radioactivity by co-injection of DTPA in [90Y-DOTA0,Tyr3]octreotide solutions containing free 90Y3+

    International Nuclear Information System (INIS)

    Peptide receptor-targeted radionuclide therapy is nowadays being performed with radiolabeled DOTA-conjugated peptides, such as [90Y-DOTA0,Tyr3]octreotide (also known as OctreoTher[reg ] or 90Y-DOTATOC). The incorporation of 90Y3+ is typically ≥99%, however, since a total patient dose can be as high as 26 GBq or 700 mCi the amount of free 90Y3+ (=non-DOTA-incorporated) can be substantial. Free 90Y3+ accumulates in bone with undesired radiation of bone marrow as a consequence. 90Y-DTPA is excreted rapidly via the kidneys. Incorporation of free 90Y3+ into 90Y-DTPA might prevent this fraction from being accumulated into bone, therefore we have investigated: the biodistribution in rats of 90YCl3, [90Y-DOTA0,Tyr3]octreotide, and 90Y-DTPA; possibilities to complex 10% of free 90Y3+ in a [90Y-DOTA0,Tyr3]octreotide containing solution into 90Y-DTPA prior to intravenous injection; and effects of 10% free 90Y3+ in [90Y-DOTA0,Tyr3]octreotide solution, in the presence and in the absence of excess DTPA, on the biodistribution of in rats. The following results are presented: 90YCl3 showed high skeletal uptake (i.e., 1% ID (injected dose) per gram femur, with main localization in the epiphyseal plates) and a 24 h total body retention of 74% ID; 90Y-DTPA had rapid renal clearance, and 24 h total body retention of 90Y3+ in [90Y-DOTA0,Tyr3]octreotide solution could rapidly be incorporated into 90Y-DTPA at room temperature; and accumulation of 90Y3+ in femur, blood, and liver was related to the amount of free 90Y3+, whereas these accumulations could be prevented by the addition of DTPA. In conclusion, the addition of excess DTPA to [90Y-DOTA0,Tyr3]octreotide with incomplete 90Y-incorporation is recommended

  18. The addition of DTPA to [177Lu-DOTA0,Tyr3]octreotate prior to administration reduces rat skeleton uptake of radioactivity

    International Nuclear Information System (INIS)

    Peptide receptor-targeted radionuclide therapy is nowadays also being performed with DOTA-conjugated peptides, such as [DOTA0,Tyr3]octreotate, labelled with radionuclides like 177Lu. The incorporation of 177Lu is typically ≥99.5%; however, since a total patient dose can be as high as 800 mCi, the amount of free 177Lu3+ (= non-DOTA-incorporated) can be substantial. Free 177Lu3+ accumulates in bone with unwanted irradiation of bone marrow as a consequence. 177Lu-DTPA is reported to be stable in serum in vitro, and in vivo it has rapid renal excretion. Transforming free Lu3+ to Lu-DTPA might reroute this fraction from accumulation in bone to renal clearance. We therefore investigated: (a) the biodistribution in rats of 177LuCl3, [177Lu-DOTA0,Tyr3]octreotate and 177Lu-DTPA; (b) the possibilities of complexing the free 177Lu3+ in [177Lu-DOTA0,Tyr3]octreotate to 177Lu-DTPA prior to intravenous injection; and (c) the effects of free 177Lu3+ in [177Lu-DOTA0,Tyr3]octreotate, in the presence and absence of DTPA, on the biodistribution in rats. 177LuCl3 had high skeletal uptake (i.e. 5% ID per gram femur, with localization mainly in the epiphyseal plates) and a 24-h total body retention of 80% injected dose (ID). [177Lu-DOTA0,Tyr3]octreotate had high and specific uptake in somatostatin receptor-positive tissues, and 24-h total body retention of 19% ID. 177Lu-DTPA had rapid renal clearance, and 24-h total body retention of 4% ID. Free 177Lu3+ in [177Lu-DOTA0,Tyr3]octreotate could be complexed to 177Lu-DTPA. Accumulation of 177Lu in femur, blood, liver and spleen showed a dose relation to the amount of free 177Lu3+, while these accumulations could be normalized by the addition of DTPA. After labelling [DOTA0,Tyr3]octreotate with 177Lu the addition of DTPA prior to intravenous administration of [177Lu-DOTA0,Tyr3]octreotate is strongly recommended. (orig.)

  19. Diagnosis und differential diagnosis of focal liver lesions using the hepatocyte-specific contrast agent Gd-EOB-DTPA; Diagnose und Differenzialdiagnose fokaler Leberlaesionen unter Verwendung von Gd-EOB-DTPA (Primovist)

    Energy Technology Data Exchange (ETDEWEB)

    Ringe, Kristina Imeen [Medizinische Hochschule Hannover (Germany). Inst. fuer Diagnostische und Interventionelle Radiologie

    2016-03-15

    The implementation of MR contrast agents significantly improves detection and characterization of focal liver lesions. This article is focusing on the hepatocyte-specific contrast agent Gd-EOB-DTPA, which has been available in Germany for liver MRI since 2004. In the introduction, the specific pharmacokinetic and -dynamic properties of this contrast agent are reviewed, and a workflow-optimized pulse sequence protocol for comprehensive hepatobiliary MRI in daily routine is presented. Afterwards, the appearance of the most common focal liver lesions is illustrated, including the differences to the use of extracellular contrast agents as well as a discussion of the most important differential diagnoses.

  20. Perspectives for Titanium-Derived Fillers Usage on Denture Base Composite Construction: A Review Article

    Directory of Open Access Journals (Sweden)

    Nidal W. Elshereksi

    2014-01-01

    Full Text Available Poly(methyl methacrylate (PMMA is an extensively used material in dentistry because of its aesthetics, processability, and reparability. However, PMMA is still far from being ideal in fulfilling the mechanical requirements of prosthesis. PMMA-based denture base polymers exhibit low fracture resistance and radiopacity behavior. Efforts to improve the mechanical and radiopacity properties of denture base materials through inclusion of silica-based fillers are ongoing. Although silane-treated siliceous fillers are commonly used, they are not sufficiently strong. They also exhibit cracks, which either cut through the glass fillers or propagate around the filler particles. This defect occurs when the dental composites are placed in aqueous oral environment because of the hydrolytic degradation of silica-based fillers and silane-coupling agents. The clinical problem of using silanes in adhesion promotion is bond degradation over time in oral environment. In addition, silanes do not bond effectively to nonsilica-based dental restorative materials. This review presents titanium-derived fillers as alternatives to siliceous fillers. Titanate-coupling agents are found to be effective couplers in treating Ti-based fillers because of their chemical compatibility and relatively high stability in aqueous environment.

  1. Hybrid method for designing digital FIR filters based on fractional derivative constraints.

    Science.gov (United States)

    Baderia, Kuldeep; Kumar, Anil; Kumar Singh, Girish

    2015-09-01

    In this manuscript, a hybrid approach based on Lagrange multiplier method and cuckoo search (CS) optimization technique is proposed for the design of linear phase finite impulse response (FIR) filters using fractional derivative constraints. In the proposed method, FIR filter is designed by optimizing the integral squares in passband and stopband from ideal response such that the fractional derivatives of designed filter response become zero at a given frequency point. Lagrange multiplier method is exploited for finding the optimized filter coefficients. Optimal value of fractional derivative constraints for optimized filter coefficients are determined by minimizing the objective function constructed using a sum of maximum passband ripple and maximum stopband ripple in frequency domain using CS algorithm. Performance of the proposed method is evaluated by passband error (ϕ(p)), stopband error (ϕ(s)), stopband attenuation (A(s)), maximum passband ripple (MPR), maximum stopband ripple (MSR) and CPU time. A comparative study of the performance of particle swarm optimization (PSO) and artificial bee colony (ABC) for designing FIR filters using the proposed method is also made. PMID:26142984

  2. Novel 2DG-based harmine derivatives for targeted cancer therapy

    Science.gov (United States)

    Wang, Aqin; Chen, Yuqi; Chen, Wei R.; Gu, Yueqing

    2013-02-01

    Harmine is a beta-carboline alkaloid from the plant Peganum harmala. These alkaloids were stimulated by their promising antitumor activities in the recent years. In this study, we designed and synthesized two harmine derivatives #1and #2 modified at position-9 of harmine with ethyl and phenylpropyl, respectively. To improve the tumor targeting capability, #1' and #2' were synthesized by conjugating 2-amino-2-deoxy-D-glucose (2DG) to the derivatives #1 and #2, respectively. The MTT assays of all these compounds in vitro against L02, HepG2 showed all compounds had low toxicity to normal cells (L02) and significantly enhanced carcinoma cell inhibitory rate compared to harmine. Cytotoxicity against liver cancer cell lines of compound #1' #2' is higher than #1 #2, and even the compound #2' is better than positive drug 5-FU. The compound #2', a novel 2DG-based harmine derivatives, could become a promising drug for targeted cancer therapy and combination therapy with other antitumor drugs.

  3. Evaluation of deriving fire cycle of forested landscape based on time-since-fire distribution

    Institute of Scientific and Technical Information of China (English)

    ZHANG Quan-fa; Kurt S. Pregitzer; JIANG Ming-xi; CHEN Wen-jun

    2006-01-01

    Estimation of fire cycle has been conducted by using the negative exponential function as an approximation of time-since-fire distribution of a landscape assumed .to be homogeneous with respect to fire spread processes. The authors imposed predefined fire cycles on a virtual landscape of 100 cell ×100 cell, and obtained a mosaic composing of patches with different stand ages (i.e. time since fire). Graphical and statistical methods (Van Wagner 1978; Reed et al. 1998) were employed to derive fire cycle from the virtual landscape. By comparing the predefined and the derived fire cycles, the two methods and tested the effects of sample size and hazard of burning (i.e., stand's susceptibility to fire in relation to its stand age) were evaluated on fire cycle deviation. The simulation results indicated a minimum sample size of 10 times of the annual burnt area would be required for partitioning time-since-fire distribution into homogeneous epochs indicating temporal change in fire cycle. Statistically, there was significant difference among the imposed and the derived fire cycle, regardless of sample sizes with or without consideration of hazard of burning. Both methods underestimated the more recent fire cycle without significant difference between them. The results imply that deviation of fire cycle based on time-since-fire distribution warrants cautious interpretation, especially when a landscape is spatially partitioned into small units and temporal changes in fire cycle are involved.

  4. Deformation analysis of polymers composites: rheological model involving time-based fractional derivative

    Science.gov (United States)

    Zhou, H. W.; Yi, H. Y.; Mishnaevsky, L.; Wang, R.; Duan, Z. Q.; Chen, Q.

    2016-08-01

    A modeling approach to time-dependent property of Glass Fiber Reinforced Polymers (GFRP) composites is of special interest for quantitative description of long-term behavior. An electronic creep machine is employed to investigate the time-dependent deformation of four specimens of dog-bond-shaped GFRP composites at various stress level. A negative exponent function based on structural changes is introduced to describe the damage evolution of material properties in the process of creep test. Accordingly, a new creep constitutive equation, referred to fractional derivative Maxwell model, is suggested to characterize the time-dependent behavior of GFRP composites by replacing Newtonian dashpot with the Abel dashpot in the classical Maxwell model. The analytic solution for the fractional derivative Maxwell model is given and the relative parameters are determined. The results estimated by the fractional derivative Maxwell model proposed in the paper are in a good agreement with the experimental data. It is shown that the new creep constitutive model proposed in the paper needs few parameters to represent various time-dependent behaviors.

  5. Detection of active bile leak with Gd-EOB-DTPA enhanced MR cholangiography: Comparison of 20–25 min delayed and 60–180 min delayed images

    Energy Technology Data Exchange (ETDEWEB)

    Cieszanowski, Andrzej, E-mail: andrzej.cieszanowski@wum.edu.pl [2nd Department of Clinical Radiology, Medical University of Warsaw, ul. Banacha 1A, 02-097 Warszawa (Poland); Stadnik, Anna, E-mail: aniaws@yahoo.com [2nd Department of Clinical Radiology, Medical University of Warsaw, ul. Banacha 1A, 02-097 Warszawa (Poland); Lezak, Aleksandra, E-mail: aleksandralezak@gmail.com [2nd Department of Clinical Radiology, Medical University of Warsaw, ul. Banacha 1A, 02-097 Warszawa (Poland); Maj, Edyta, E-mail: em26@wp.pl [2nd Department of Clinical Radiology, Medical University of Warsaw, ul. Banacha 1A, 02-097 Warszawa (Poland); Zieniewicz, Krzysztof, E-mail: krzysztof.zieniewicz@wum.edu.pl [Chair and Department of General, Transplant and Liver Surgery, Medical University of Warsaw, ul. Banacha 1A, 02-097 Warszawa (Poland); Rowinska-Berman, Katarzyna, E-mail: kasiarowinska@wp.pl [2nd Department of Clinical Radiology, Medical University of Warsaw, ul. Banacha 1A, 02-097 Warszawa (Poland); Grudzinski, Ireneusz P., E-mail: ireneusz.grudzinski@wum.edu.pl [Department of Toxicology, Medical University of Warsaw, Faculty of Pharmacy, ul. Banacha 1, 02-097 Warsaw (Poland); Krawczyk, Marek, E-mail: marek.krawczyk@wum.edu.pl [Chair and Department of General, Transplant and Liver Surgery, Medical University of Warsaw, ul. Banacha 1A, 02-097 Warszawa (Poland); Rowiński, Olgierd, E-mail: olgierd.rowinski@wum.edu.pl [2nd Department of Clinical Radiology, Medical University of Warsaw, ul. Banacha 1A, 02-097 Warszawa (Poland)

    2013-12-01

    Objectives: The purpose of this study was to assess the value of contrast-enhanced magnetic resonance cholangiography (MRC) performed in different time delays after injection of gadoxetic acid disodium (Gd-EOB-DTPA) for the diagnosis of active bile leak. Methods: This retrospective analysis included Gd-EOB-DTPA enhanced MR images of 34 patients suspected of bile leak. Images were acquired 20–25 min after Gd-EOB-DTPA injection. If there was inadequate contrast in the bile ducts then delayed images after 60–90 min and 150–180 min were obtained. Results were correlated with intraoperative findings, ERCP results, clinical data, laboratory tests, and follow-up examinations. Results: Gd-EOB-DTPA enhanced MRC yielded an overall sensitivity of 96.4%, specificity of 100% and accuracy of 97.1% for the diagnosis of an active bile leak. The sensitivity of 20–25 min delayed MR images was 42.9%, of combined 20–25 min and 60–90 min delayed images was 92.9% and of combined 20–25 min, 60–90 min and 150–180 min delayed images was 96.4%. Conclusions: Gd-EOB-DTPA enhanced MRC utilizing delayed phase images was effective for detecting the presence and location of active bile leaks. The images acquired 60–180 min post-injection enabled identification of bile leaks even in patients with a dilated biliary system or moderate liver dysfunction.

  6. Development of Ni-Based Catalysts for Steam Reforming of Tar Derived from Biomass Pyrolysis

    Institute of Scientific and Technical Information of China (English)

    Dalin LI; Yoshinao NAKAGAWA; Keiichi TOMISHIGE

    2012-01-01

    Nickel catalysts are effective for the steam reforming of tar derived from biomass pyrolysis,but the improvement is needed in terms of activity,stability,suppression of coke deposition and aggregation,and regeneration.Our recent development of Ni-based catalysts for the steam reforming of tar is reviewed including the modification with CeO2 (or MnO),trace Pt,and MgO.The role of additives such as CeO2,MnO,Pt,and MgO is also discussed.

  7. [Separation of enantiomers by supercritical fluid chromatography on polysaccharide derivative-based chiral stationary phases].

    Science.gov (United States)

    Li, Dongyan; Wu, Xi; Hao, Fangli; Yang, Yang; Chen, Xiaoming

    2016-01-01

    Eleven kinds of chiral compounds have been well separated within 10 min on polysaccharide derivative-based chiral stationary phases named Chiralpak IA, IB, IC, ID, IE and IF by supercritical fluid chromatography (SFC). The chiral recognition of these chiral compounds has demonstrated good complementary enantioselectivities of the six chiral columns, which were proved to be useful for chiral SFC. Both the elution time and enantioselectivies could be significantly affected by the modifier types and their concentrations, such as methanol, ethanol and isopropanol, which should be optimized during the experiments. In addition, the solvent versatility of the immobilized chiral stationary phase on the optimization of the chiral separation was helpful. PMID:27319169

  8. Integral Theorems Based on a New Gradient Operator Derived from Biomembranes (Part Ⅱ): Applications

    Institute of Scientific and Technical Information of China (English)

    YIN Yajun

    2005-01-01

    Based on the second gradient operator and corresponding integral theorems such as the second divergence theorem, the second gradient theorem, the second curl theorem, and the second circulation theorem on curved surfaces, a few new scalar differential operators are defined and a series of integral transformations are derived. Interesting transformations between the average curvature and the Gauss curvature are presented. Various conserved integrals related to the Gauss curvature and the second fundamental tensor are disclosed. The important applications of the results in disciplines such as the geometry, physics, mechanics, and biology are briefly discussed.

  9. Complexes of trivalent lanthanide ions with schiff base derived from vanillin and triethylenetetraamine

    International Nuclear Information System (INIS)

    Complexes of lanthanide(III) ions with the schiff base derived from vanillin and triethylenetetraamine have been synthesised and characterised on the basis of elemental analyses, molar conductance, magnetic moment, IR and thermal analysis data. The thermograms show the elimination (OH)(OCH3)C6H3CH-group at low temperatures before the elimination of triethylenetetraamine part corroborating the observation made on the basis of IR spectral data. This is a clear indication of the non-coordination of the phenolic hydroxyl groups. The lanthanide ions in the complexes exhibit eight coordination numbers. (author)

  10. THE TEMPERATURE-BASED DERIVATIVES CONTRACTS – NEW PRODUCTS OF WEATHER RISK INDUSTRY

    OpenAIRE

    Mirela MATEI; Cătălin VOICA

    2011-01-01

    The temperature-based derivatives contracts have appeared while the first deregulations in the utilities and the energy sectors from USA in the years 1990-2000. The transfer of the some services in the private sector has involved a better control of costs and revenues of different companies. The private investors who have turned to this area, have imposed a risk reduction management more complex than that used by any public company, in order to ensure their profits and a smaller variation of ...

  11. Electrochemical Biosensors Based on Ferroceneboronic Acid and Its Derivatives: A Review

    Directory of Open Access Journals (Sweden)

    Baozhen Wang

    2014-07-01

    Full Text Available We review recent progress in the development of electrochemical biosensors based on ferroceneboronic acid (FcBA and ferrocene (Fc-modified boronic acids. These compounds can be used to construct electrochemical biosensors because they consist of a binding site (i.e., a boronic acid moiety and an electrochemically active part (i.e., an Fc residue. By taking advantage of the unique properties of FcBA and its derivatives, electrochemical sensors sensitive to sugars, glycated hemoglobin (HbA1c, fluoride (F− ions, and so forth have been widely studied. FcBA-based sugar sensors rely on the selective binding of FcBA to 1,2- or 1,3-diol residues of sugars through the formation of cyclic boronate ester bonds. The redox properties of FcBA-sugar adduct differ from those of free FcBA, which forms the basis of the electrochemical determination of sugars. Thus, non-enzymatic glucose sensors are now being actively studied using FcBA and Fc-modified boronic acids as redox markers. Using a similar principle, HbA1c can be detected by FcBA-based electrochemical systems because it contains hydrocarbon chains on the polypeptide chain. HbA1c sensors are useful for monitoring blood glucose levels over the preceding 8–12 weeks. In addition, FcBA and Fc-modified boronic acids have been used for the detection of F− ions due to the selective binding of boronic acid to F− ions. F−-ion sensors may be useful alternatives to conventional ion-selective electrodes sensitive to F− ion. Furthermore, FcBA derivatives have been studied to construct lectin; steroids; nucleotides; salicylic acid; and bacteria sensors. One of the limitations of FcBA-based sensors comes from the fact that FcBA derivatives are added in sample solutions as reagents. FcBA derivatives should be immobilized on the surface of electrodes for developing reagentless sensors.

  12. Gaussian higher Order Derivative based Structural Enhancement of Digital Bone X-Ray Images

    Directory of Open Access Journals (Sweden)

    Raka Kundu

    2011-01-01

    Full Text Available A novel method for enhancement of digital X-ray images of bones is presented in this paper. It has come to observation that the proposed method based on the Gaussian higher order derivative shows an appreciable enhancement of edges in digital X-ray images of bones that can be used for detection of various bone deformities as well as for the better understanding of the bone structure. We have achieved a level of improvement in distinguishing the bone information from the other parts of the digital X-ray images.

  13. A derives grid-based model for simulation of pedestrian flow

    Institute of Scientific and Technical Information of China (English)

    Min-jie CHEN; Günter B(A)RWOLFF; Hartmut SCHWANDT

    2009-01-01

    We present a derived grid-based model for the simulation of pedestrian flow. Interactions among pedestrians are considered as the result of forces within a certain neighbourhood. Unlike the social force model, the forces here, as in Newtonian physics, are proportional to the inverse of the square of the distance. Despite the notion of neighbourhood and the underlying grid, this model differs from the existing cellular automaton (CA) models in that the pedestrians are treated as individuals. Bresenham's algorithm for line rastering is applied in the step calculation.

  14. Synthesis, characterization and dynamic NMR studies of a novel chalcone based N-substituted morpholine derivative

    Science.gov (United States)

    Baskar, R.; Baby, C.; Moni, M. S.; Subramanian, K.

    2013-05-01

    The synthesis of a novel chalcone based N-substituted morpholine derivative namely, (E)-1-(biphenyl-4-yl)-3-(4-(5-morpholinopentyloxy) phenyl) prop-2-en-1-one (BMPP), using a two step protocol is reported. The compound is characterized by FTIR, GC-MS and FTNMR spectroscopy techniques. Advanced 2D NMR techniques such as gradient enhanced COSY, HSQC, HMBC and NOESY were employed to establish through-bond and through-space correlations. Dynamic NMR measurements were carried out to obtain the energy barrier to ring inversion of the morpholine moiety.

  15. An efficient nonviral gene-delivery vector based on hyperbranched cationic glycogen derivatives

    Directory of Open Access Journals (Sweden)

    Liang X

    2014-01-01

    Full Text Available Xuan Liang,1,* Xianyue Ren,2,* Zhenzhen Liu,1 Yingliang Liu,1 Jue Wang,2 Jingnan Wang,2 Li-Ming Zhang,1 David YB Deng,2 Daping Quan,1 Liqun Yang1 1Institute of Polymer Science, School of Chemistry and Chemical Engineering, Key Laboratory of Designed Synthesis and Application of Polymer Material, Key Laboratory for Polymeric Composite and Functional Materials of Ministry of Education, Sun Yat-Sen University, Guangzhou, People's Republic of China; 2Research Center of Translational Medicine, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou, People's Republic of China *Both these authors contributed equally to this work Background: The purpose of this study was to synthesize and evaluate hyperbranched cationic glycogen derivatives as an efficient nonviral gene-delivery vector. Methods: A series of hyperbranched cationic glycogen derivatives conjugated with 3-(dimethylamino-1-propylamine (DMAPA-Glyp and 1-(2-aminoethyl piperazine (AEPZ-Glyp residues were synthesized and characterized by Fourier-transform infrared and hydrogen-1 nuclear magnetic resonance spectroscopy. Their buffer capacity was assessed by acid–base titration in aqueous NaCl solution. Plasmid deoxyribonucleic acid (pDNA condensation ability and protection against DNase I degradation of the glycogen derivatives were assessed using agarose gel electrophoresis. The zeta potentials and particle sizes of the glycogen derivative/pDNA complexes were measured, and the images of the complexes were observed using atomic force microscopy. Blood compatibility and cytotoxicity were evaluated by hemolysis assay and MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide assay, respectively. pDNA transfection efficiency mediated by the cationic glycogen derivatives was evaluated by flow cytometry and fluorescence microscopy in the 293T (human embryonic kidney and the CNE2 (human nasopharyngeal carcinoma cell lines. In vivo delivery of pDNA in model animals (Sprague Dawley

  16. A rapid method for assessing ventilation post-perfusion: The use of Tc-99m DTPA aerosol and computer subtraction

    International Nuclear Information System (INIS)

    Aerosol ventilation studies currently require considerable cooperation on the part of the patient and may result in lengthy study periods or potentially high doses of radiation. This report validates an aerosol method which minimizes patients study time and attempts to keep radiation exposure to a minimum. Seventeen patients underwent a control pre-perfusion Tc-99m DTPA aerosol multi-view study prior to a 2 mCi Tc-99m MMA multi-view perfusion study. The patient was then placed in an ''optimum position'' to maximize a visual abnormality seen on perfusion. Nebulized Tc-99m DTPA at two times the original concentration for the pre-perfusion aerosol was again inhaled. Images were obtained on film for two minutes and on computer in one minute frame increments for two minutes. The ''perfusion'' image consisted of counts from the Tc-99m MAA and Tc-99m DTPA pre-perfusion aerosol. This image was used as a ''mask'' and subtracted from the post-perfusion aerosol image. The resultant image represented the post-perfusion aerosol counts only. With the patient acting as his own control all 17 patients demonstrated no significant differences between the pre-perfusion image and the post perfusion computer subtracted image. The authors conclude that post-perfusion aerosol imaging equivalent to pre-perfusion may be obtained using as little as one minute of imaging time. An entire study can be performed in 5-6 minutes or less allowing for all of the advantages of post-perfusion ventilation imaging while not sacrificing the quality of a pre-perfusion test

  17. Successful DTPA therapy in the case of 239Pu penetration via injured skin exposed to nitric acid

    International Nuclear Information System (INIS)

    This paper presents results of the radiological study and DTPA therapy for a worker exposed to a plutonium nitrate solution. Plutonium levels were measured in excreta, blood, plasma and wound for several weeks. Plutonium renal clearance ranged from 110-190 ml min-1 to 3-4 ml min-1 at different stages of chelation therapy. Plutonium absorption into blood from the injured skin amounted to 4.3%. As a result of intensive therapy, 96% of absorbed plutonium was successfully excreted. (author)

  18. Capillary permeability of 99mTc-DTPA in canine myocardium determined by intracoronary bolus injection and residue detection

    DEFF Research Database (Denmark)

    Svendsen, Jesper Hastrup; Efsen, F; Haunsø, S

    1989-01-01

    Capillary permeability of 99mtechnetium-diethylenetriaminepenta-acetic acid (99mTc-DTPA; MW 485.0) and 51chromium-ethylenediaminetetra-acetate (51Cr-EDTA; MW 340.2) was studied in an in vivo canine heart preparation by the single injection, residue detection (SIRD) method. In experiments on open...... chest dogs (group A) these indicators were administered separately as bolus injections into a cannulated diagonal branch of the left anterior descending coronary artery (LAD) and the curve of the response function was recorded by external activity registration. In further experiments on closed and open...

  19. Spectral Characterization and Antimicrobial Activity of Some Schiff Bases Derived from 4-Chloro-2-aminophenol and Various Salicylaldehyde Derivatives%Spectral Characterization and Antimicrobial Activity of Some Schiff Bases Derived from 4-Chloro-2-aminophenol and Various Salicylaldehyde Derivatives

    Institute of Scientific and Technical Information of China (English)

    Cinarli, Adem; Gurbuz, Demet; Tavman, Aydin; Birteksoz, A. Seher

    2012-01-01

    A series of N-(5-chloro-2-hydroxyphenyl)-(3/4/5-substituted)-salicylaldimines (I--XI) were synthesized using appropriate synthetic route. Their structures were characterized by FT-IR, UV-Visible, ESI-MS, 1H and 13C NMR spectroscopic techniques and analytical methods. The crystal structure of N-(5-chloro-2-hydroxyphenyl)-5-bromo- salicylaldimine (V) was determined by X-ray diffraction at room temperature. Relationship between the melting points and the structures of the compounds was examined. Antimicrobial activity of the compounds was evaluated against Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Proteus mirabilis. Antifungal activities were reported for Candida albicans. Schiff bases showed considerable antimicrobial activity against S. aureus, S, epidermidis and C. albicans. N-(5-Chloro-2-hydroxyphenyl)-3-hydroxy-salieylaldimine (II) has the broadest and highest antimicrobial activity according to the others.

  20. Potential biological applications of bio-based anacardic acids and their derivatives.

    Science.gov (United States)

    Hamad, Fatma B; Mubofu, Egid B

    2015-01-01

    Cashew nut shells (CNS), which are agro wastes from cashew nut processing factories, have proven to be among the most versatile bio-based renewable materials in the search for functional materials and chemicals from renewable resources. CNS are produced in the cashew nut processing process as waste, but they contain cashew nut shell liquid (CNSL) up to about 30-35 wt. % of the nut shell weight depending on the method of extraction. CNSL is a mixture of anacardic acid, cardanol, cardol, and methyl cardol, and the structures of these phenols offer opportunities for the development of diverse products. For anacardic acid, the combination of phenolic, carboxylic, and a 15-carbon alkyl side chain functional group makes it attractive in biological applications or as a synthon for the synthesis of a multitude of bioactive compounds. Anacardic acid, which is about 65% of a CNSL mixture, can be extracted from the agro waste. This shows that CNS waste can be used to extract useful chemicals and thus provide alternative green sources of chemicals, apart from relying only on the otherwise declining petroleum based sources. This paper reviews the potential of anacardic acids and their semi-synthetic derivatives for antibacterial, antitumor, and antioxidant activities. The review focuses on natural anacardic acids from CNS and other plants and their semi-synthetic derivatives as possible lead compounds in medicine. In addition, the use of anacardic acid as a starting material for the synthesis of various biologically active compounds and complexes is reported. PMID:25894225

  1. Prevention of post weaning diarrhoea by a Saccharomyces cerevisiae-derived product based on whole yeast

    DEFF Research Database (Denmark)

    Jensen, K. H.; Damgaard, B. M.; Andresen, Lars Ole;

    2013-01-01

    The aim of this study was to examine whether yeast derivate (YD) based on whole brewery yeast added to the creep feed of suckling and newly weaned piglets or to the creep feed of the piglets and the sow's diet prevented post weaning diarrhoea (PWD) or affected performance. Thirty sows and their l......The aim of this study was to examine whether yeast derivate (YD) based on whole brewery yeast added to the creep feed of suckling and newly weaned piglets or to the creep feed of the piglets and the sow's diet prevented post weaning diarrhoea (PWD) or affected performance. Thirty sows...... and their litters were randomly allocated to three treatment groups: PSP (1.5 g/kg of YD to the sows’ feed from 1 wk before expected farrowing to weaning; 3 g/kg or 2 g/kg of YD added to the piglets’ creep feed from 2 wk of age until 2 wk post weaning (PW) and from wk 2 to 5 PW, respectively); PP (YD added...... from each litter. In individually housed piglets the faecal consistency score (FCS) was affected by an interaction between days PW, treatment group, and challenge group (P=0.005). In general, FCS was lower in placebo than in E. coli-challenged piglets and in PSP and PP piglets than in C piglets...

  2. Toward Multiple Conductance Pathways with Heterocycle-Based Oligo(phenyleneethynylene) Derivatives.

    Science.gov (United States)

    Miguel, Delia; Álvarez de Cienfuegos, Luis; Martín-Lasanta, Ana; Morcillo, Sara P; Zotti, Linda A; Leary, Edmund; Bürkle, Marius; Asai, Yoshihiro; Jurado, Rocío; Cárdenas, Diego J; Rubio-Bollinger, Gabino; Agraït, Nicolás; Cuerva, Juan M; González, M Teresa

    2015-11-01

    In this paper, we have systematically studied how the replacement of a benzene ring by a heterocyclic compound in oligo(phenyleneethynylene) (OPE) derivatives affects the conductance of a molecular wire using the scanning tunneling microscope-based break junction technique. We describe for the first time how OPE derivatives with a central pyrimidine ring can efficiently link to the gold electrode by two pathways presenting two different conductance G values. We have demonstrated that this effect is associated with the presence of two efficient conductive pathways of different length: the conventional end-to-end configuration, and another with one of the electrodes linked directly to the central ring. This represents one of the few examples in which two defined conductive states can be set up in a single molecule without the aid of an external stimulus. Moreover, we have observed that the conductance through the full length of the heterocycle-based OPEs is basically unaffected by the presence of the heterocycle. All these results and the simplicity of the proposed molecules push forward the development of compounds with multiple conductance pathways, which would be a breakthrough in the field of molecular electronics. PMID:26452050

  3. Potential Biological Applications of Bio-Based Anacardic Acids and Their Derivatives

    Directory of Open Access Journals (Sweden)

    Fatma B. Hamad

    2015-04-01

    Full Text Available Cashew nut shells (CNS, which are agro wastes from cashew nut processing factories, have proven to be among the most versatile bio-based renewable materials in the search for functional materials and chemicals from renewable resources. CNS are produced in the cashew nut processing process as waste, but they contain cashew nut shell liquid (CNSL up to about 30–35 wt. % of the nut shell weight depending on the method of extraction. CNSL is a mixture of anacardic acid, cardanol, cardol, and methyl cardol, and the structures of these phenols offer opportunities for the development of diverse products. For anacardic acid, the combination of phenolic, carboxylic, and a 15-carbon alkyl side chain functional group makes it attractive in biological applications or as a synthon for the synthesis of a multitude of bioactive compounds. Anacardic acid, which is about 65% of a CNSL mixture, can be extracted from the agro waste. This shows that CNS waste can be used to extract useful chemicals and thus provide alternative green sources of chemicals, apart from relying only on the otherwise declining petroleum based sources. This paper reviews the potential of anacardic acids and their semi-synthetic derivatives for antibacterial, antitumor, and antioxidant activities. The review focuses on natural anacardic acids from CNS and other plants and their semi-synthetic derivatives as possible lead compounds in medicine. In addition, the use of anacardic acid as a starting material for the synthesis of various biologically active compounds and complexes is reported.

  4. Vitamin E derivative-based multifunctional nanoemulsions for overcoming multidrug resistance in cancer.

    Science.gov (United States)

    Zheng, Nannan; Gao, Yanan; Ji, Hongyu; Wu, Linhua; Qi, Xuejing; Liu, Xiaona; Tang, Jingling

    2016-08-01

    The multidrug resistance (MDR), including intrinsic and acquired multidrug resistance, is a major problem in tumor chemotherapy. Here, we proposed a strategy for modulating intrinsic and/or acquired multidrug resistance by altering the levels of Bax and Bcl-2 expression and inhibiting the transport function of P-gp, increasing the intracellular concentration of its substrate anticancer drugs. Vitamin E derivative-based nanoemulsions containing paclitaxel (MNEs-PTX) were fabricated in this study, and in vitro anticancer efficacy of the nanoemulsion system was evaluated in the paclitaxel-resistant human ovarian carcinoma cell line A2780/Taxol. The MNEs-PTX exhibited a remarkably enhanced antiproliferation effect on A2780/Taxol cells than free paclitaxel (PTX) (p Vitamin E derivative-based multifunctional nanoemulsion (MNEs) obviously increased intracellular accumulation of rhodamine 123 (P-gp substrate). Overexpression of Bcl-2 is generally associated with tumor drug resistance, we found that MNEs could reduce Bcl-2 protein level and increase Bax protein level. Taken together, our findings suggest that anticancer drugs associated with MNEs could play a role in the development of MDR in cancers. PMID:26710274

  5. Development and Characterization of Novel Films Based on Sulfonamide-Chitosan Derivatives for Potential Wound Dressing.

    Science.gov (United States)

    Dragostin, Oana Maria; Samal, Sangram Keshari; Lupascu, Florentina; Pânzariu, Andreea; Dubruel, Peter; Lupascu, Dan; Tuchilus, Cristina; Vasile, Cornelia; Profire, Lenuta

    2015-01-01

    The objective of this study was to develop new films based on chitosan functionalized with sulfonamide drugs (sulfametoxydiazine, sulfadiazine, sulfadimetho-xine, sulfamethoxazol, sulfamerazine, sulfizoxazol) in order to enhance the biological effects of chitosan. The morphology and physical properties of functionalized chitosan films as well the antioxidant effects of sulfonamide-chitosan derivatives were investigated. The chitosan-derivative films showed a rough surface and hydrophilic properties, which are very important features for their use as a wound dressing. The film based on chitosan-sulfisoxazol (CS-S6) showed the highest swelling ratio (197%) and the highest biodegradation rate (63.04%) in comparison to chitosan film for which the swelling ratio was 190% and biodegradation rate was only 10%. Referring to the antioxidant effects the most active was chitosan-sulfamerazine (CS-S5) which was 8.3 times more active than chitosan related to DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging ability. This compound showed also a good ferric reducing power and improved total antioxidant capacity. PMID:26694354

  6. GNSS-SNR-derived water surface heights based on Newton Interval Analysis

    Science.gov (United States)

    Reinking, Jörg

    2016-04-01

    The power of Global navigation satellite system (GNSS) signals is commonly recorded as signal-to-noise ratio (SNR) by GNSS receivers. SNR mainly depends on the direct signal but also on the reflected signal. Hence the analysis of SNR data allows the computation of heights of the reflecting surfaces by means of interference pattern technique (IPT). In classical IPT the distance between the antenna and the reflector is derived from the multipath pattern using a Lomb-Scargle Periodogram (LSP) analysis which is calculated separately for every satellite involved. The final reflector height is later estimated combining all those results A more sophisticated approach uses a consistent computation of the reflector height from all observations of all satellites in a single estimation step. This is achieved by replacing LSP analysis by an appropriate common least squares adjustment for all satellites. The sum of squares of residuals from such an adjustment depends on the reflector height and is used as an objective function. The reflector height is than derived in a global optimization process based on interval analysis. This approach additionally reduces the computational efforts compared to LSP. For a constant or only slowly changing reflector height, the height could be treated at least as a quasi-static non-time-depending function for a particular time window. In this one-dimensional case the global optimization can be carried out based on the Interval Newton Method. The method is demonstrated using a data set obtain from a measurement on the Weser river, Germany.

  7. Decomposition driven interface evolution for layers of binary mixtures: I. Model derivation and stratified base states

    CERN Document Server

    Thiele, Uwe; Frastia, Lubor

    2007-01-01

    A dynamical model is proposed to describe the coupled decomposition and profile evolution of a free surface film of a binary mixture. An example is a thin film of a polymer blend on a solid substrate undergoing simultaneous phase separation and dewetting. The model is based on model-H describing the coupled transport of the mass of one component (convective Cahn-Hilliard equation) and momentum (Navier-Stokes-Korteweg equations) supplemented by appropriate boundary conditions at the solid substrate and the free surface. General transport equations are derived using phenomenological non-equilibrium thermodynamics for a general non-isothermal setting taking into account Soret and Dufour effects and interfacial viscosity for the internal diffuse interface between the two components. Focusing on an isothermal setting the resulting model is compared to literature results and its base states corresponding to homogeneous or vertically stratified flat layers are analysed.

  8. ATLID beam steering mechanism and derived new piezoelectric-based devices for optical applications

    Science.gov (United States)

    Bourgain, F.; Barillot, F.; Belly, C.; Claeyssen, F.

    2015-09-01

    In Space & Defence (as well as in many others fields), there is a trend for miniaturisation in active optics requiring new actuators. Applications also often require the ability to withstand high vibrations and shocks levels, as well as vacuum compatibility for space applications. A new generation of small and smart actuators such as piezoelectric (piezo) actuators, are resolving this trend, thanks to their capacity to offer high energy density and to support both extreme and various requirements. This paper first presents the BSM mechanism and its requirements, the technologies involved in the design and the validation campaign results. Secondly, a derived XY piezoelectric positioning stage based on the same APA® and associated Strain Gage sensing technology is presented with its associated performances. Finally, a new piezoelectric motor based on the APA® technology, which allows the combination of long stroke while maintaining high resolution positioning of optical elements, is presented with experimental performances.

  9. Liquid chromatographic determination of beta-cyclodextrin derivatives based on fluorescence enhancement after inclusion complexation.

    Science.gov (United States)

    Reeuwijk, H J; Irth, H; Tjaden, U R; Merkus, F W; van der Greef, J

    1993-04-21

    A liquid chromatographic method using fluorescence detection for the determination of beta-cyclodextrin (beta CD) and its derivatives is presented. The chromatographic system is based on size-exclusion chromatography with the addition of the fluorophoric compound 1-naphthol to the mobile phase. Detection is based on fluorescence enhancement caused by the formation of inclusion complexes. By incorporating 10(-4) M 1-naphthol in the mobile phase, detection limits of 90, 27, 370 and 37 pmol were obtained for beta CD, hydroxypropyl-beta CD, trimethyl-beta CD and dimethyl-beta CD, respectively. The method was applied to the determination of dimethyl-beta CD in urine: the minimum detectable concentration was 0.2 microgram/ml after preconcentration of 10 ml of urine.

  10. Antibacterial Evaluation of Some Schiff Bases Derived from 2-Acetylpyridine and Their Metal Complexes

    Directory of Open Access Journals (Sweden)

    Thong Kwai Lin

    2012-05-01

    Full Text Available A series of Schiff bases derived from 2-acetylpyridne and their metal complexes were characterized by elemental analysis, NMR, FT-IR and UV-Vis spectral studies. The complexes were screened for anti-bacterial activity against Methicillin-resistant Staphylococcus aureus (MRSA, Acinetobacter baumanni (AC, Klebsiella pneumonie (KB and Pseudomonas aeruginosa (PA using the disc diffusion and micro broth dilution assays. Based on the overall results, the complexes showed the highest activities against MRSA while a weak antibacterial activity was observed against A. baumanii and P. aeruginosa.

  11. Characterization of ι-carrageenan and its derivative based green polymer electrolytes

    Energy Technology Data Exchange (ETDEWEB)

    Jumaah, Fatihah Najirah; Mobaraka, Nadhratun Naiim; Ahmad, Azizan; Ramli, Nazaruddin [School of Chemical Sciences and Food Technology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600, Bangi, Selangor Darul Ehsan (Malaysia)

    2013-11-27

    The new types of green polymer electrolytes based on ι-carrageenan derivative have been prepared. ι-carrageenan act as precursor was reacted with monochloroacetic acid to produce carboxymethyl ι-carrageenan. The powders were characterized by Attenuated Total Reflection Fourier Transform infrared (ATR-FTIR) spectroscopy and {sup 1}H nuclear magnetic resonance (NMR) to confirm the substitution of targeted functional group in ι-carrageenan. The green polymer electrolyte based on ι-carrageenan and carboxymethyl ι-carrageenan was prepared by solution-casting technique. The films were characterized by electrochemical impedance spectroscopy to determine the ionic conductivity. The ionic conductivity ι-carrageenan film were higher than carboxymethyl ι-carrageenan which 4.87 ×10{sup −6} S cm{sup −1} and 2.19 ×10{sup −8} S cm{sup −1}, respectively.

  12. Synthesis, Radiolabelling and In Vitro Characterization of the Gallium-68-, Yttrium-90- and Lutetium-177-Labelled PSMA Ligand, CHX-A''-DTPA-DUPA-Pep

    Directory of Open Access Journals (Sweden)

    Benjamin Baur

    2014-04-01

    Full Text Available Since prostate-specific membrane antigen (PSMA has been identified as a diagnostic target for prostate cancer, many urea-based small PSMA-targeting molecules were developed. First, the clinical application of these Ga-68 labelled compounds in positron emission tomography (PET showed their diagnostic potential. Besides, the therapy of prostate cancer is a demanding field, and the use of radiometals with PSMA bearing ligands is a valid approach. In this work, we describe the synthesis of a new PSMA ligand, CHX-A''-DTPA-DUPA-Pep, the subsequent labelling with Ga-68, Lu-177 and Y-90 and the first in vitro characterization. In cell investigations with PSMA-positive LNCaP C4-2 cells, KD values of ≤14.67 ± 1.95 nM were determined, indicating high biological activities towards PSMA. Radiosyntheses with Ga-68, Lu-177 and Y-90 were developed under mild reaction conditions (room temperature, moderate pH of 5.5 and 7.4, respectively and resulted in nearly quantitative radiochemical yields within 5 min.

  13. Usefulness of sup(99m)Tc-DTPA renogram in the functional and diagnostic evaluations of chronic renal insufficiency in children

    Energy Technology Data Exchange (ETDEWEB)

    Matsueda, Y.; Hiraiwa, M.; Meguro, H.; Fujii, R. (Teikyo Univ., Tokyo (Japan). Faculty of Medicine)

    1980-09-01

    As there are some difficulties in the performance of renal biopsy and intravenous pyelography is of little use in children with chronic renal insufficiency, we evaluated the usefulness of sup(99m)Tc-DTPA renogram in two children. The causes of the renal insufficiency presented were obstructive congenital anomalies and chronic pyelonephritis in one patient and polycystic kidneys in the other. As expected, intravenous pyelography poorly visualized in the upper urinary tracts of both the cases examined. Sup(99m)Tc-DTPA renogram gave us distinct image for the diagnosis of the kidneys and urinary tracts, and the individual renal functions were clarified as well. It was apparently superior to the combined use of sup(99m)Tc-DMSA renoscintigram and /sup 131/I-Hippuran renogram in both the imaging and functional evaluations. In such cases as are reported herein, sup(99m)Tc-DTPA renogram might be a useful alternative to several other diagnostic tests.

  14. Discordant uptake of Tc-99m DTPA-galactosyl human serum albumin and Tc-99m Sn colloid in a patient with severe acute hepatitis.

    Science.gov (United States)

    Miyazaki, C; Matsunaga, T; Kubo, K

    1994-08-01

    A patient with recently diagnosed severe acute hepatitis underwent serial liver scintigraphy with Tc-99m Sn colloid and Tc-99m DTPA-galactosyl human serum albumin. In initial studies, radionuclide distribution on Tc-99m DTPA-galactosyl human serum albumin scintigraphy was completely discrepant to that on Tc-99m Sn colloid scintigraphy. In a follow-up study 1 month later, the distribution of both radionuclides in the liver appeared relatively homogeneous. The uptake of Tc-99m DTPA-galactosyl human serum albumin and Tc-99m Sn colloid reflects the function of hepatocytes and Kupffer cells, respectively. Both kinds of scintigraphic study may be helpful to assess histopathologic change of different hepatic tissue architectures. PMID:7955747

  15. An efficient hybrid causative event-based approach for deriving the annual flood frequency distribution

    Science.gov (United States)

    Thyer, Mark; Li, Jing; Lambert, Martin; Kuczera, George; Metcalfe, Andrew

    2015-04-01

    Flood extremes are driven by highly variable and complex climatic and hydrological processes. Derived flood frequency methods are often used to predict the flood frequency distribution (FFD) because they can provide predictions in ungauged catchments and evaluate the impact of land-use or climate change. This study presents recent work on development of a new derived flood frequency method called the hybrid causative events (HCE) approach. The advantage of the HCE approach is that it combines the accuracy of the continuous simulation approach with the computational efficiency of the event-based approaches. Derived flood frequency methods, can be divided into two classes. Event-based approaches provide fast estimation, but can also lead to prediction bias due to limitations of inherent assumptions required for obtaining input information (rainfall and catchment wetness) for events that cause large floods. Continuous simulation produces more accurate predictions, however, at the cost of massive computational time. The HCE method uses a short continuous simulation to provide inputs for a rainfall-runoff model running in an event-based fashion. A proof-of-concept pilot study that the HCE produces estimates of the flood frequency distribution with similar accuracy as the continuous simulation, but with dramatically reduced computation time. Recent work incorporated seasonality into the HCE approach and evaluated with a more realistic set of eight sites from a wide range of climate zones, typical of Australia, using a virtual catchment approach. The seasonal hybrid-CE provided accurate predictions of the FFD for all sites. Comparison with the existing non-seasonal hybrid-CE showed that for some sites the non-seasonal hybrid-CE significantly over-predicted the FFD. Analysis of the underlying cause of whether a site had a high, low or no need to use seasonality found it was based on a combination of reasons, that were difficult to predict apriori. Hence it is recommended

  16. Non-invasive detection of biliary leaks using Gd-EOB-DTPA-enhanced MR cholangiography: comparison with T2-weighted MR cholangiography

    Energy Technology Data Exchange (ETDEWEB)

    Kantarci, Mecit; Pirimoglu, Berhan; Bayraktutan, Ummugulsum; Ogul, Hayri; Kizrak, Yesim; Eren, Suat [Atatuerk University, School of Medicine, Department of Radiology, Erzurum (Turkey); Karabulut, Nevzat [Pamukkale University, School of Medicine, Department of Radiology, Denizli (Turkey); Ozturk, Gurkan; Aydinli, Bulent [Atatuerk University, School of Medicine, Department of General Surgery, Erzurum (Turkey); Yilmaz, Sinan [Atatuerk University, School of Medicine, Department of Public Health, Erzurum (Turkey)

    2013-10-15

    To evaluate the added role of T1-weighted (T1w) gadolinium ethoxybenzyl diethylenetriamine penta-acetic acid (Gd-EOB-DTPA)-enhanced magnetic resonance cholangiography (MRC) compared with T2-weighted MRC (T2w-MRC) in the detection of biliary leaks. Ninety-nine patients with suspected biliary complications underwent routine T2w-MRC and T1w contrast-enhanced (CE) MRC using Gd-EOB-DTPA to identify biliary leaks. Two observers reviewed the image sets separately and together. MRC findings were compared with those of surgery and percutaneous transhepatic cholangiopancreatography. The sensitivity, specificity and accuracy of the techniques in identifying biliary leaks were calculated. Accuracy of locating biliary leaks was superior with the combination of Gd-EOB-DTPA-enhanced MRC and T2w-MRC (P < 0.05).The mean sensitivities were 79 % vs 59 %, and the mean accuracy rates were 84 % vs 58 % for combined CE-MRC and T2w-MRC vs sole T2w-MRC. Nineteen out of 21 patients with biliary-cyst communication, 90.4 %, and 12/15 patients with post-traumatic biliary extravasations, 80 %, were detected by the combination of Gd-EOB-DTPA-enhanced MRC and T2w-MRC images, P < 0.05. Gd-EOB-DTPA-enhanced MRC yields information that complements T2w-MRC findings and improves the identification and localisation of the bile extravasations (84 % accuracy, 100 % specificity, P < 0.05). We recommend Gd-EOB-DTPA-enhanced MRC in addition to T2w-MRC to increase the preoperative accuracy of identifying and locating extravasations of bile. (orig.)

  17. Analyzing Signal Attenuation in PFG Anomalous Diffusion via a Modified Gaussian Phase Distribution Approximation Based on Fractal Derivative Model

    CERN Document Server

    Lin, Guoxing

    2016-01-01

    Pulsed field gradient (PFG) has been increasingly employed to study anomalous diffusions in Nuclear Magnetic Resonance (NMR) and Magnetic Resonance Imaging (MRI). However, the analysis of PFG anomalous diffusion is complicated. In this paper, a fractal derivative model based modified Gaussian phase distribution method is proposed to describe PFG anomalous diffusion. By using the phase distribution obtained from the effective phase shift diffusion method based on fractal derivatives, and employing some of the traditional Gaussian phase distribution approximation techniques, a general signal attenuation expression for free fractional diffusion is derived. This expression describes a stretched exponential function based attenuation, which is distinct from both the exponential attenuation for normal diffusion obtained from conventional Gaussian phase distribution approximation, and the Mittag-Leffler function based attenuation for anomalous diffusion obtained from fractional derivative. The obtained signal attenu...

  18. Antipathogenic effects of structurally-related Schiff base derivatives: Structure–activity relationship

    Directory of Open Access Journals (Sweden)

    Soleiman Hisaindee

    2015-11-01

    Full Text Available Eighteen structurally-related Schiff base derivatives, which belong to salicylidenebenzylamine and 2-hydroxy-1-naphthylidenebenzylamine families were prepared and characterized by spectroscopic techniques. All the synthesized compounds were screened in vitro for their antibacterial and antifungal activities. Human pathogenic gram-negative (Escherichia coli, Proteus mirabilis, Pseudomonas aeruginosa and Serratia marcescens, gram-positive bacteria (Staphylococcus epidermidis, Staphylococcus aureus and Bacillus subtilis, and fungi (Alternaria alternata, Aspergillus niger, Penicillium roqueforti, and Saccharomyces cerevisiae were evaluated based on their toxicity to different concentrations of Schiff base compounds. For assessment of toxicity to pathogens, a disc diffusion assay was used to test the antimicrobial properties. The results revealed some antimicrobial activities of some of the synthesized compounds. Among the tested pathogens, the synthesized salicylidinaniline derivatives show highly potent action towards Alternaria alternata. Interestingly, a compound which contains the –Cl group is only effective against gram negative bacteria, but not gram positive bacteria. No remarkable antibacterial or antifungal activities were observed in the presence of –CH3 or –Br group. Furthermore, most of the naphthalene-containing compounds show no growth retardation towards bacterial or fungal pathogens. On the other hand, the presence of –OH or –SH group at para or ortho position, respectively, on the aniline site is correlated with an increased inhibitory drug effect on all pathogens. The importance of our findings to the drug research and developments is discussed in the context of finding a correlation between the structural properties of the new drugs and their biological activities.

  19. A membrane-based purification process for cell culture-derived influenza A virus.

    Science.gov (United States)

    Weigel, Thomas; Solomaier, Thomas; Wehmeyer, Sebastian; Peuker, Alessa; Wolff, Michael W; Reichl, Udo

    2016-02-20

    A simple membrane-based purification process for cell culture-derived influenza virus was established that relies on only two chromatographic unit operations to achieve the contamination limits required according to regulatory authorities. After clarification and concentration, a pseudo-affinity membrane adsorber (sulfated cellulose, SCMA) was applied for virus capture. The subsequent polishing step consisted of a salt-tolerant anion exchange membrane adsorber (STMA) to bind residual DNA. For the presented process neither a buffer exchange step nor a nuclease step for further DNA digestion were required. As a starting point, a two-salt strategy (including a polyvalent ion) was employed to screen STMA conditions in a 96-well plate format. After optimization on chromatographic laboratory scale, the virus recovery was up to 97% with a residual DNA level below 0.82%. In addition, the STMA was characterized regarding its dynamic binding capacity and the impact of flow rate on yields and contamination levels. Overall, the total virus yield for influenza virus A/PR/8/34 (H1/N1) of this two-step membrane process was 75%, while the protein and the DNA contamination level could be reduced to 24% and at least 0.5%, respectively. With 19.8μg protein and 1.2ng DNA per monovalent dose, this purity level complies with the limits of the European Pharmacopeia for cell culture-derived vaccines for human use. Overall, the presented downstream process might serve as a generic and economic platform technology for production of cell culture-derived viruses and viral vectors. PMID:26712479

  20. Research progress in muscle-derived stem cells Literature retrieval results based on international database

    Institute of Scientific and Technical Information of China (English)

    Li Zhang; Wei Wang

    2012-01-01

    OBJECTIVE: To identify global research trends of muscle-derived stem cells (MDSCs) using a bibliometric analysis of the Web of Science, Research Portfolio Online Reporting Tools of the National Institutes of Health (NIH), and the Clinical Trials registry database (ClinicalTrials.gov). DATA RETRIEVAL: We performed a bibliometric analysis of data retrievals for MDSCs from 2002 to 2011 using the Web of Science, NIH, and ClinicalTrials.gov. MAIN OUTCOME MEASURES: (1) Type of literature; (2) annual publication output; (3) distribution according to journals; (4) distribution according to country; (5) distribution according to institution; (6) top cited authors over the last 10 years; (7) projects financially supported by the NIH; and (8) clinical trials registered.RESULTS: (1) In all, 802 studies on MDSCs appeared in the Web of Science from 2002 to 2011, almost half of which derived from American authors and institutes. The number of studies on MDSCs has gradually increased over the past 10 years. Most papers on MDSCs appeared in journals with a particular focus on cell biology research, such as Experimental Cell Research, Journal of Cell Science, and PLoS One. (2) Eight MDSC research projects have received over US$6 billion in funding from the NIH. The current project led by Dr. Johnny Huard of the University of Pittsburgh-"Muscle-Based Tissue Engineering to Improve Bone Healing"-is supported by the NIH. Dr. Huard has been the most productive and top-cited author in the field of gene therapy and adult stem cell research in the Web of Science over last 10 years. (3) On ClinicalTrials.gov, "Muscle Derived Cell Therapy for Bladder Exstrophy Epispadias Induced Incontinence" Phase 1 is registered and sponsored by Johns Hopkins University and has been led by Dr. John P. Gearhart since November 2009.CONCLUSION: From our analysis of the literature and research trends, we found that MDSCs may offer further benefits in regenerative medicine.

  1. Synthesis, characterization and antibacterial studies of ruthenium(III) complexes derived from chitosan schiff base.

    Science.gov (United States)

    Vadivel, T; Dhamodaran, M

    2016-09-01

    Chitosan can be modified chemically by condensation reaction of deacetylated chitosan with aldehyde in homogeneous phase. This condensation is carried by primary amine (NH2) with aldehyde (CHO) to form corresponding schiff base. The chitosan biopolymer schiff base derivatives are synthesized with substituted aldehydes namely 4-hydroxy-3-methoxy benzaldehyde, 2-hydroxy benzaldehyde, and 2-hydroxy-3-methoxy benzaldehyde, becomes a complexing agent or ligand. The Ruthenium(III) complexes were obtained by complexation of Ruthenium with schiff base ligands and this product exhibits as an excellent solubility and more biocompatibility. The novel series of schiff base Ruthenium(III) complexes are characterized by Elemental analysis, FT-IR spectroscopy, and Thermo-gravimetric analysis (TGA). The synthesized complexes have been subjected to antibacterial study. The antibacterial results indicated that the antibacterial activity of the complexes were more effective against Gram positive and Gram negative pathogenic bacteria. These findings are giving suitable support for developing new antibacterial agent and expand our scope for applications. PMID:26562551

  2. Robust Analysis of Network-Based Real-Time Kinematic for GNSS-Derived Heights.

    Science.gov (United States)

    Bae, Tae-Suk; Grejner-Brzezinska, Dorota; Mader, Gerald; Dennis, Michael

    2015-10-26

    New guidelines and procedures for real-time (RT) network-based solutions are required in order to support Global Navigation Satellite System (GNSS) derived heights. Two kinds of experiments were carried out to analyze the performance of the network-based real-time kinematic (RTK) solutions. New test marks were installed in different surrounding environments, and the existing GPS benchmarks were used for analyzing the effect of different factors, such as baseline lengths, antenna types, on the final accuracy and reliability of the height estimation. The RT solutions are categorized into three groups: single-base RTK, multiple-epoch network RTK (mRTN), and single-epoch network RTK (sRTN). The RTK solution can be biased up to 9 mm depending on the surrounding environment, but there was no notable bias for a longer reference base station (about 30 km) In addition, the occupation time for the network RTK was investigated in various cases. There is no explicit bias in the solution for different durations, but smoother results were obtained for longer durations. Further investigation is needed into the effect of changing the occupation time between solutions and into the possibility of using single-epoch solutions in precise determination of heights by GNSS.

  3. Detection of an acute occlusion of the renal artery in a kidney transplant using sup(99m)Tc-DTPA sequential scintiscanning

    International Nuclear Information System (INIS)

    131I-hippurane isotope nephrography is a well-tried method to control the function of a kidney transplant. The method is well suited for an early observation of shedding crises. In addition, glomerullary filtered sup(99m)Tc-DTPA may be used to detect vascular complications (renal arteriostenosis, occlusion of renal arteries, renal venal thromboses, aneurysm) and disturbances of the potrenal outflow. Vascular complications in kidney transplants shortly after surgery are possible; they require a quick and precise diagnosis. Perfusion analysis with the aid of sup(99m)Tc-DTPA as a non-invasive and hardly exerting method is frequently used for diagnosis of this kind. (orig.)

  4. MR angiography of collateral arteries in a hind limb ischemia model: comparison between blood pool agent Gadomer and small contrast agent Gd-DTPA.

    Directory of Open Access Journals (Sweden)

    Karolien Jaspers

    Full Text Available The objective of this study was to compare the blood pool agent Gadomer with a small contrast agent for the visualization of ultra-small, collateral arteries (diameter0.10. Inter-observer variation was 24% and 18% for Gadomer and Gd-DTPA, respectively. In conclusion, blood pool agent Gadomer improved vessel conspicuity compared to Gd-DTPA. Steady-state MRA can be considered as an excellent non-invasive alternative to intra-arterial XRA for the visualization of ultra-small collateral arteries.

  5. White light generation by carbonyl based indole derivatives due to proton transfer: an efficient fluorescence sensor.

    Science.gov (United States)

    Singla, Nidhi; Bhadram, Venkata Srinu; Narayana, Chandrabhas; Chowdhury, Papia

    2013-04-01

    The motivation of the present work is to understand the optical, chemical, and electrical aspects of the proton transfer mechanism of indole (I) and some carbonyl based indole derivatives: indole-3-carboxaldehyde (I3C) and indole-7-carboxaldehyde (I7C) for both powder form and their liquid solution. Structural information for indole derivatives (isolated molecule and in solution) is obtained with density functional theory (DFT) and time dependent DFT (TD-DFT) methods. Calculated transition energies are used to generate UV-vis, FTIR, Raman, and NMR spectra which are later verified with the experimental spectra. The occurrence of different conformers [cis (N(c)), trans (N(t)), and zwitterion (Z*)] have been interpreted by Mulliken charge, natural bond orbital (NBO) analysis, and polarization versus electric field (P-E loop) studies. (1)H and (13)C NMR and molecular vibrational frequencies of the fundamental modes established the stability of Nc due to the presence of intramolecular hydrogen bonding (IHB) in the ground state (S0). Computed/experimental UV-vis absorption/emission studies reveal the creation of new species: zwitterion (Z*) and anion (A*) in the excited state (S1) due to excited state intramolecular and intermolecular proton transfer (ESI(ra)PT and ESI(er)PT). Increased electrical conductivity (σ(ac)) with temperature and increased ferroelectric polarization at higher field verifies proton conduction in I7C.

  6. Properties of Thermosets Derived from Chemically Modified Triglycerides and Bio-Based Comonomers

    Directory of Open Access Journals (Sweden)

    Evan S. Beach

    2013-12-01

    Full Text Available A series of materials was prepared by curing acrylated epoxidized soybean oil (AESO and dibutyl itaconate (DBI or ethyl cinnamate (EC comonomers to provide examples of thermosets with a high proportion of bio-based carbon, in accordance with the principles of green chemistry. The comonomers, representative of cellulose-derived (DBI or potentially lignin-derived (EC raw materials, were tested at levels of 25%, 33%, and 50% by mass and the resulting products were characterized by infrared spectroscopy, thermogravimetric analysis, and dynamic mechanical analysis. Both DBI and EC were incorporated into the thermosets to a high extent (>90% at all concentrations tested. The AESO-DBI and AESO-EC blends showed substantial degradation at 390–400 °C, similar to pure AESO. Glass transition temperatures decreased as comonomer content increased; the highest Tg of 41.4 °C was observed for AESO-EC (3:1 and the lowest Tg of 1.4 °C was observed for AESO-DBI (1:1. Accordingly, at 30 °C the storage modulus values were highest for AESO-EC (3:1, 37.0 MPa and lowest for AESO-DBI (1:1, 1.5 MPa.

  7. Polyurethane and polyurea nanoparticles based on polyoxyethylene castor oil derivative surfactant suitable for endovascular applications.

    Science.gov (United States)

    Morral-Ruíz, Genoveva; Melgar-Lesmes, Pedro; García, María Luísa; Solans, Conxita; García-Celma, María José

    2014-01-30

    The design of new, safe and effective nanotherapeutic systems is an important challenge for the researchers in the nanotechnology area. This study describes the formation of biocompatible polyurethane and polyurea nanoparticles based on polyoxyethylene castor oil derivative surfactant formed from O/W nano-emulsions by polymerization at the droplet interfaces in systems composed by aqueous solution/Kolliphor(®) ELP/medium chain triglyceride suitable for intravenous administration. Initial nano-emulsions incorporating highly hydrophilic materials were prepared by the phase inversion composition (PIC) method. After polymerization, nanoparticles with a small particle diameter (25-55 nm) and low polydispersity index were obtained. Parameters such as concentration of monomer, O/S weight ratio as well as the polymerization temperature were crucial to achieve a correct formation of these nanoparticles. Moreover, FT-IR studies showed the full conversion of the monomer to polyurethane and polyurea polymers. Likewise the involvement of the surfactant in the polymerization process through their nucleophilic groups to form the polymeric matrix was demonstrated. This could mean a first step in the development of biocompatible systems formulated with polyoxyethylene castor oil derivative surfactants. In addition, haemolysis and cell viability assays evidenced the good biocompatibility of KELP polyurethane and polyurea nanoparticles thus indicating the potential of these nanosystems as promising drug carriers.

  8. FORTRAN program for analyzing ground-based radar data: Usage and derivations, version 6.2

    Science.gov (United States)

    Haering, Edward A., Jr.; Whitmore, Stephen A.

    1995-01-01

    A postflight FORTRAN program called 'radar' reads and analyzes ground-based radar data. The output includes position, velocity, and acceleration parameters. Air data parameters are also provided if atmospheric characteristics are input. This program can read data from any radar in three formats. Geocentric Cartesian position can also be used as input, which may be from an inertial navigation or Global Positioning System. Options include spike removal, data filtering, and atmospheric refraction corrections. Atmospheric refraction can be corrected using the quick White Sands method or the gradient refraction method, which allows accurate analysis of very low elevation angle and long-range data. Refraction properties are extrapolated from surface conditions, or a measured profile may be input. Velocity is determined by differentiating position. Accelerations are determined by differentiating velocity. This paper describes the algorithms used, gives the operational details, and discusses the limitations and errors of the program. Appendices A through E contain the derivations for these algorithms. These derivations include an improvement in speed to the exact solution for geodetic altitude, an improved algorithm over earlier versions for determining scale height, a truncation algorithm for speeding up the gradient refraction method, and a refinement of the coefficients used in the White Sands method for Edwards AFB, California. Appendix G contains the nomenclature.

  9. Investigations on the Ga(III) Complex of EOB-DTPA and Its 68Ga Radiolabeled Analogue.

    Science.gov (United States)

    Greiser, Julia; Niksch, Tobias; Weigand, Wolfgang; Freesmeyer, Martin

    2016-01-01

    We demonstrate a method for the isolation of EOB-DTPA (3,6,9-triaza-3,6,9-tris(carboxymethyl)-4-(ethoxybenzyl)-undecanedioic acid) from its Gd(III) complex and protocols for the preparation of its novel non-radioactive, i.e., natural Ga(III) as well as radioactive (68)Ga complex. The ligand as well as the Ga(III) complex were characterized by nuclear magnetic resonance (NMR) spectroscopy, mass spectrometry and elemental analysis. (68)Ga was obtained by a standard elution method from a (68)Ge/(68)Ga generator. Experiments to evaluate the (68)Ga-labeling efficiency of EOB-DTPA at pH 3.8-4.0 were performed. Established analysis techniques radio TLC (thin layer chromatography) and radio HPLC (high performance liquid chromatography) were used to determine the radiochemical purity of the tracer. As a first investigation of the (68)Ga tracers' lipophilicity the n-octanol/water distribution coefficient of (68)Ga species present in a pH 7.4 solution was determined by an extraction method. In vitro stability measurements of the tracer in various media at physiological pH were performed, revealing different rates of decomposition. PMID:27584545

  10. Synthesis and relaxivity of Gd(Ⅲ), Fe(Ⅲ) and Mn(Ⅱ)complexes with dihydropyridine derivative of diethylenetriaminepentaacetic acid

    Institute of Scientific and Technical Information of China (English)

    CHANG; Jianhua; ZHENG; Shuzhan; JIAN; Yajun; BAI; Yinjuan

    2005-01-01

    A novel ligand of DTPA-dihydropyridine derivative was synthesized by reaction of DTPA-dianhydride with 4-aniline-1,4-dihydropyridine. Its complexes of gadolinium, manganese and iron were prepared. Their spin-lattice relaxivities (T1) were investigated. The results show that the NMR T1 relaxivitives (R1) for complexes of Fe(Ⅲ), Mn(Ⅱ) are less than that of Gd(Ⅲ) complex,which has a high relaxivity (R1) on the surrounding water protons, indicating that the Gd(Ⅲ) complex possesses the precondition to be contrast agents for magnetic resonance imaging.

  11. Tc-99m DTPA perfusion scintigraphy and color coded duplex sonography in the evaluation of minimal renal allograft perfusion

    Energy Technology Data Exchange (ETDEWEB)

    Bair, H.J.; Platsch, G.; Wolf, F. [Erlangen-Nuernberg Univ., Erlangen (Germany). Dept. of Nuclear Medicine; Guenter, E.; Becker, D. [Erlangen-Nuernberg Univ., Erlangen (Germany). Dept. of Internal Medicine 1; Rupprecht, H.; Neumayer, H.H. [Erlangen-Nuernberg Univ., Erlangen (Germany). Dept. of Internal Medicine 4

    1997-08-01

    Aim: The clinical impact of perfusion scintigraphy versus color coded Duplex sonography was evaluated, with respect to their potential in assessing minimal allograft perfusion in vitally threatened kidney transplants, i.e. oligoanuric allografts suspected to have either severe rejection or thrombosis of the renal vein or artery. Methods: From July 1990 to August 1994 the grafts of 15 out of a total of 315 patients were vitally threatened. Technetium-99m DTPA scintigraphy and color coded Duplex sonography were performed in all patients. For scintigraphic evaluation of transplant perfusion analog scans up to 60 min postinjection, and time-activity curves over the first 60 sec after injection of 370-440 MBq Tc-99m diethylenetriaminepentaacetate acid (DTPA) were used and classified by a perfusion score, the time between renal and iliac artery peaks (TDiff) and the washout of the renogram curve. Additionally, evaluation of excretion function and assessment of vascular or urinary leaks were performed. By color coded Duplex sonography the perfusion in all sections of the graft as well as the vascular anastomoses were examined and the maximal blood flow velocity (Vmax) and the resistive index (RI) in the renal artery were determined by means of the pulsed Doppler device. Pathologic-anatomical diagnosis was achieved by either biopsy or post-explant histology in all grafts. Results: Scintigraphy and color coded Duplex sonography could reliably differentiate minimal (8/15) and not perfused (7/15) renal allografts. The results were confirmed either by angiography in digital subtraction technique (DSA) or the clinical follow up. Conclusion: In summary, perfusion scintigraphy and color coded Duplex sonography are comparable modalities to assess kidney graft perfusion. In clinical practice scintigraphy and colorcoded Doppler sonography can replace digital subtraction angiography in the evaluation of minimal allograft perfusion. (orig.) [Deutsch] Ziel der Studie war es, das

  12. Poly-Acrylic Acid Derivatives as Diesel Flow Improver for Paraffin-Based Daqing Diesel

    Institute of Scientific and Technical Information of China (English)

    Cuiyu Jiang; Ming Xu; Xiaoli Xi; Panlun Qi; Hongyan Shang

    2006-01-01

    Since the diesel products from paraffin-based Daqing crude oil showed low sensitivity to certain commercial diesel pour point depressant (PPDs) that resulted from the high content of paraffin, certain poly-acrylic acid derivatives (PADE) with-COOR,-COOH,-CONHR, and -COO-NH3+R groups by molecular design on the mechanics of diesel; PPDs were synthesized and evaluated as cold flow improver for Daqing 0# diesel in this paper. The pure PADE was superior to the commercial PPDs and displayed a substantial ability of wax crystals dispersion. There was a synergistic effect among the PADE and T1804 and secondary amine. The synergism clearly improved the low temperature performance of Daqing diesel products and could reduce the cold filter plugging point of 0# diesel by 6-7 ℃.

  13. Catecholase activity investigations using in situ copper complexes continuing Schiff base derivatives with a theoretical calculation

    Directory of Open Access Journals (Sweden)

    A. Djedouani

    2015-03-01

    Full Text Available The study of catecholase activity of a series of Schiff base compounds using in situ copper complexes of 4-hydroxy-6-methyl-3-(1-(phenyliminoethyl-2H-pyran-2-one derivatives has been reported. The reaction rate depends on four parameters: The nature of the substitution in para position to the benzene ring, the nature of counter anion, the concentration of ligand and the nature of solvent. The highest rate activity is given by complex resulting from one equivalent of ligand L2 and two equivalents of copper acetate in methanol, which equal to 62.25 µmol.min-1.L-1.In other part, a theoretical study of such ligands using the semi-empirical method AM1 were also investigated. A good relationship founded between the maximal reaction rate (Vmax and the HOMO energy (Pearson correlation: r=-0.794.

  14. Smart photovoltaics based on dye-sensitized solar cells using photochromic spiropyran derivatives as photosensitizers

    International Nuclear Information System (INIS)

    In this paper, smart photovoltaic (SPV) devices, integrating both functions of solar cells and smart windows, was fabricated based on dye-sensitized solar cells using photochromic spiropyran derivatives SIBT as photosensitizers. SPV devices have self-regulated power conversion efficiency (PCE) and light transmission responding to the incident spectra due to the photoisomerization of SIBT. SIBT isomerize from closed-ring form to open-ring form under UV illumination, accompanied with enhanced visible light absorption and electron delocalization. Therefore, increased PCE and absorption in SPV devices were observed under UV treatment and the devices can be restored gradually to the initial status when kept in dark. The SPV devices have self-regulation of PCE and sunlight transmission responding to the changing sun spectra in different times of a day, providing a proper energy usage and a better sun-shading

  15. Derivation of the Newton's Law of Gravitation Based on a Fluid Mechanical Singularity Model of Particles

    Directory of Open Access Journals (Sweden)

    Xiao-Song Wang

    2008-10-01

    Full Text Available The main purpose of this paper is to seek a mechanical interpretation of gravitational phenomena. We suppose that the universe may be filled with a kind of fluid which may be called the (0 substratum. Thus, the inverse-square law of gravitation is derived by methods of hydrodynamics based on a sink flow model of particles. The first feature of this theory of gravitation is that the gravitational interactions are transmitted by a kind of fluidic medium. The second feature is the time dependence of gravitational constant G and gravitational mass. The Newton’s law of gravitation is arrived if we introduce an assumption that G and the masses of particles are changing so slowly that they can be treated as constants.

  16. Image Clustering Method Based on Density Maps Derived from Self-Organizing Mapping: SOM

    Directory of Open Access Journals (Sweden)

    Kohei Arai

    2012-07-01

    Full Text Available A new method for image clustering with density maps derived from Self-Organizing Maps (SOM is proposed together with a clarification of learning processes during a construction of clusters. It is found that the proposed SOM based image clustering method shows much better clustered result for both simulation and real satellite imagery data. It is also found that the separability among clusters of the proposed method is 16% longer than the existing k-mean clustering. It is also found that the separability among clusters of the proposed method is 16% longer than the existing k-mean clustering. In accordance with the experimental results with Landsat-5 TM image, it takes more than 20000 of iteration for convergence of the SOM learning processes.

  17. Smart photovoltaics based on dye-sensitized solar cells using photochromic spiropyran derivatives as photosensitizers

    Energy Technology Data Exchange (ETDEWEB)

    Ma, Shengbo; Ting, Hungkit; Ma, Yingzhuang; Zheng, Lingling; Zhang, Miwei [State Key Laboratory for Artificial Microstructures and Mesoscopic Physics, Department of Physics, Peking University, Beijing 100871 (China); Xiao, Lixin, E-mail: zjchen@pku.edu.cn, E-mail: lxxiao@pku.edu.cn; Chen, Zhijian, E-mail: zjchen@pku.edu.cn, E-mail: lxxiao@pku.edu.cn [State Key Laboratory for Artificial Microstructures and Mesoscopic Physics, Department of Physics, Peking University, Beijing 100871 (China); Haixi Collaborative Innovation Center for New Display Devices and Systems Integration, Fuzhou University, Fuzhou 350002 (China)

    2015-05-15

    In this paper, smart photovoltaic (SPV) devices, integrating both functions of solar cells and smart windows, was fabricated based on dye-sensitized solar cells using photochromic spiropyran derivatives SIBT as photosensitizers. SPV devices have self-regulated power conversion efficiency (PCE) and light transmission responding to the incident spectra due to the photoisomerization of SIBT. SIBT isomerize from closed-ring form to open-ring form under UV illumination, accompanied with enhanced visible light absorption and electron delocalization. Therefore, increased PCE and absorption in SPV devices were observed under UV treatment and the devices can be restored gradually to the initial status when kept in dark. The SPV devices have self-regulation of PCE and sunlight transmission responding to the changing sun spectra in different times of a day, providing a proper energy usage and a better sun-shading.

  18. Prediction of rumen microbial outflow based on urinary excretion of purine derivatives

    International Nuclear Information System (INIS)

    The method for predicting microbial protein outflow from the rumen based on the excretion of purine derivatives (PD) in the urine is being increasingly used by nutritionists. In contrast to methods that depend on estimates of digesta flow, the PD method does not require animals to be fitted surgically with cannulae into the gut, and studies can be performed with minimal disturbance to the experimental animals. Methods of analysis of PD have been improved and standardized. Certain assumptions, however, are required that could lead to errors when this method is used to predict microbial protein outflow from the rumen. The need for further investigation of these assumptions by means of isotopic tracers and other techniques is examined. (author)

  19. The first genetic linkage map of Primulina eburnea (Gesneriaceae) based on EST-derived SNP marker

    Indian Academy of Sciences (India)

    CHEN FENG; CHAO FENG; MING KANG

    2016-06-01

    Primulina eburneais a promising candidate for domestication and floriculture, since it is easy to culture and has beautiful flow-ers. An F2population of 189 individuals was established for the construction of first-generation linkage maps based onexpressed sequence tags-derived single-nucleotide polymorphism markers using the massARRAY genotyping platform. Ofthe 232 screened markers, 215 were assigned to 18 LG according to the haploid number of chromosomes in the species. Thelinkage map spanned a total of 3774.7 cM with an average distance of 17.6 cM between adjacent markers. This linkage mapprovides a framework for identification of important genes in breeding programm

  20. STRUCTURE BASED DRUG DESIGN STUDIES ON HETEROARYL PROPANOIC ACID DERIVATIVES AS PPARΓ AGONISTS

    Directory of Open Access Journals (Sweden)

    ARUN KUMAR,MANGA RATNAM,ARCHANA KUMARI, AJAY BABU

    2013-09-01

    Full Text Available Peroxisome proliferator-activated receptors (PPARs are a group of nuclear receptorproteins. Docking studies are based on several factors. Among 15 entries of PPARγ, 2Q6S wastaken for docking analysis, as it showed 418 most favored regions, 35 in additionally allowedregion and none of the residue in disallowed regions. To carry out drug designing, moleculeswere considered from the literature in which substitution of R1 position with dihydrofurylreported to have high dock score (-14.98 Kcal/mol than the remaining analogues, with bettergeometry and interactions. Hence docking analysis using heteroaryl propionic acid derivatives asanti-diabetic agents suggest the reproducibility of active molecules being predicted bycomputational docking studies using Auto dock software.

  1. Design of Assembled Systems Based on Conjugated Polyphenylene Derivatives and Carbon Nanohorns.

    Science.gov (United States)

    Iglesias, Daniel; Guerra, Javier; Gómez, M Victoria; Rodríguez, Antonio M; Prieto, Pilar; Vázquez, Ester; Herrero, M Antonia

    2016-08-01

    Promising materials have been designed and fully characterised by an effective interaction between versatile platforms such as carbon nanohorns (CNHs) and conjugated molecules based on thiophene derivatives. Easy and non-aggressive methods have been described for the synthesis and purification of the final systems. Oligothiophenephenylvinylene (OTP) systems with different geometries and electron density are coupled to the CNHs. A wide range of characterization techniques have been used to confirm the effective interaction between the donor (OTP) and the acceptor (CNH) systems. These hybrid materials show potential for integration into solar cell devices. Importantly, surface-enhanced Raman spectroscopy (SERS) effects are observed without the presence of any metal surface in the system. Theoretical calculations have been performed to study the optimised geometries of the noncovalent interaction between the surface and the organic molecule. The calculations allow information on the monoelectronic energies of HOMO-LUMO orbitals and band gap of different donor systems to be extracted. PMID:27404562

  2. Derivation of the Newton's Law of Gravitation Based on a Fluid Mechanical Singularity Model of Particles

    Directory of Open Access Journals (Sweden)

    Xiao-Song Wang

    2008-08-01

    Full Text Available The main purpose of this paper is to seek a mechanical interpretation of gravitational phenomena. We suppose that the universe may be filled with a kind of fluid which may be called the (0 substratum. Thus, the inverse-square law of gravitation is derived by methods of hydrodynamics based on a sink flow model of particles. The first feature of this theory of gravitation is that the gravitational interactions are transmitted by a kind of fluidic medium. The second feature is the time dependence of gravitational constant G and gravitational mass. The Newton’s law of gravitation is arrived if we introduce an assumption that G and the masses of particles are changing so slowly that they can be treated as constants.

  3. Naturally derived materials-based cell and drug delivery systems in skin regeneration.

    Science.gov (United States)

    Huang, Sha; Fu, Xiaobing

    2010-03-01

    The objective of regenerative medicine is to provide cells with a local environment of artificial extracellular matrix where they can proliferate and differentiate efficiently and therefore, induce the repair of defective tissues according to the natural healing potential of patients. For this purpose, naturally derived materials are being widely used because of their similarities to the extracellular matrix, typically good biocharacteristics and inherent cellular interaction. Also, natural polymers can be engineered to release growth factors and related agents in response to physiologic signals to imitate the natural healing process and to promote fast tissue regeneration and reduce scarring in wounds. Although synthetic materials have been used extensively in tissue engineering fields, this review illustrates the contribution of natural materials and natural materials-based protein delivery systems to regenerative medicine research, with emphasis on the application of multifunctional vehicles for cell and growth factor delivery in skin regeneration research. PMID:19850093

  4. Smart photovoltaics based on dye-sensitized solar cells using photochromic spiropyran derivatives as photosensitizers

    Directory of Open Access Journals (Sweden)

    Shengbo Ma

    2015-05-01

    Full Text Available In this paper, smart photovoltaic (SPV devices, integrating both functions of solar cells and smart windows, was fabricated based on dye-sensitized solar cells using photochromic spiropyran derivatives SIBT as photosensitizers. SPV devices have self-regulated power conversion efficiency (PCE and light transmission responding to the incident spectra due to the photoisomerization of SIBT. SIBT isomerize from closed-ring form to open-ring form under UV illumination, accompanied with enhanced visible light absorption and electron delocalization. Therefore, increased PCE and absorption in SPV devices were observed under UV treatment and the devices can be restored gradually to the initial status when kept in dark. The SPV devices have self-regulation of PCE and sunlight transmission responding to the changing sun spectra in different times of a day, providing a proper energy usage and a better sun-shading.

  5. Recent progress in electrochemical biosensors based on phenylboronic acid and derivatives.

    Science.gov (United States)

    Anzai, Jun-Ichi

    2016-10-01

    This review provides an overview of recent progress made in the development of electrochemical biosensors based on phenylboronic acid (PBA) and its derivatives. PBAs are known to selectively bind 1,2- and 1,3-diols to form negatively charged boronate esters in neutral aqueous media and have been used to construct electrochemical glucose sensors because of this selective binding. PBA-modified metal and carbon electrodes have been widely studied as voltammetric and potentiometric glucose sensors. In some cases, ferroceneboronic acid or ferrocene-modified phenylboronic acids are used as sugar-selective redox compounds. Another option for sensors using PBA-modified electrodes is potentiometric detection, in which the changes in surface potential of the electrodes are detected as an output signal. An ion-sensitive field effect transistor (FET) has been used as a signal transducer in potentiometric sensors. Glycoproteins, such as glycated hemoglobin (HbA1c), avidin, and serum albumin can also be detected by PBA-modified electrodes because they contain hydrocarbon chains on the surface. HbA1c sensors are promising alternatives to enzyme-based glucose sensors for monitoring blood glucose levels over the preceding 2-3months. In addition, PBA-modified electrodes can be used to detect a variety of compounds including hydroxy acids and fluoride (F(-)) ions. PBA-based F(-) ion sensors may be useful if reagentless sensors can be developed. PMID:27287174

  6. An efficient causative event-based approach for deriving the annual flood frequency distribution

    Science.gov (United States)

    Li, Jing; Thyer, Mark; Lambert, Martin; Kuczera, George; Metcalfe, Andrew

    2014-03-01

    In ungauged catchments or catchments without sufficient streamflow data, derived flood frequency methods are often applied to provide the basis for flood risk assessment. The most commonly used event-based methods, such as design storm and joint probability approaches are able to give fast estimation, but can also lead to prediction bias and uncertainties due to the limitations of inherent assumptions and difficulties in obtaining input information (rainfall and catchment wetness) related to events that cause extreme floods. An alternative method is a long continuous simulation which produces more accurate predictions, but at the cost of massive computational time. In this study a hybrid method was developed to make the best use of both event-based and continuous approaches. The method uses a short continuous simulation to provide inputs for a rainfall-runoff model running in an event-based fashion. The total probability theorem is then combined with the peak over threshold method to estimate annual flood distribution. A synthetic case study demonstrates the efficacy of this procedure compared with existing methods of estimating annual flood distribution. The main advantage of the hybrid method is that it provides estimates of the flood frequency distribution with an accuracy similar to the continuous simulation approach, but with dramatically reduced computation time. This paper presents the method at the proof-of-concept stage of development and future work is required to extend the method to more realistic catchments.

  7. Microcalorimetric Study on the Inhibition of Escherichia coil by Some Novel Pyridine Amide Schiff Base Derivatives

    Institute of Scientific and Technical Information of China (English)

    LI Chao-Hong; ZHANG Li-Xia; CAI Li-Hua; LIU Yi; HU Pei-Zhi

    2008-01-01

    The antibacterial effect of a series of novel pyridine amide Schiff base compounds on Escherichia coli was investigated by a microcalorimetric method at 37℃.The metabolic power-time curves of the bacteria treated by the compounds were obtained,and the thermokinetic parameters were analyzed,from which the antibacterial activities of these compounds were evaluated.The results show that two compounds F and G have good activity on aerobic multiplying metabolism of E.coli,with the value of IC50 106 and 113 mg/L respectively,but have no effective action on the fermentation metabolism of E.coli.The action of the compounds on the non-multiplying metabolism was investigated by taking the heat output of E.coli in the stationary phase as the guideline of the activity.The experimental results revealed that the hydrophilicity of these Schiff bases had a great influence on their antibacterial activity,which results from the bacterial cell wall structure.The antibacterial structure-activity relationship of these Schiff base derivatives was also briefly discussed.The antibacterial activity of the compounds against E.coli was as follows:compound F>G>C>D>E>B>A.

  8. Deriving statistical significance maps for SVM based image classification and group comparisons.

    Science.gov (United States)

    Gaonkar, Bilwaj; Davatzikos, Christos

    2012-01-01

    Population based pattern analysis and classification for quantifying structural and functional differences between diverse groups has been shown to be a powerful tool for the study of a number of diseases, and is quite commonly used especially in neuroimaging. The alternative to these pattern analysis methods, namely mass univariate methods such as voxel based analysis and all related methods, cannot detect multivariate patterns associated with group differences, and are not particularly suitable for developing individual-based diagnostic and prognostic biomarkers. A commonly used pattern analysis tool is the support vector machine (SVM). Unlike univariate statistical frameworks for morphometry, analytical tools for statistical inference are unavailable for the SVM. In this paper, we show that null distributions ordinarily obtained by permutation tests using SVMs can be analytically approximated from the data. The analytical computation takes a small fraction of the time it takes to do an actual permutation test, thereby rendering it possible to quickly create statistical significance maps derived from SVMs. Such maps are critical for understanding imaging patterns of group differences and interpreting which anatomical regions are important in determining the classifier's decision.

  9. Synthesis of Urea based Chalcone Derivatives and Evaluate its Biological Activity

    OpenAIRE

    Arpita Desai; Vyas, K. B.; Rajarshi N. Patel; K. S. Nimavat

    2016-01-01

    Chalcones have been the center of attraction for researchers from several decades due to nits innumerous therapeutic application, Efforts have been done in my research to synthesized chalcones and their derivatives that further reacts with various substituted aldehyde to give corresponding substituted chalcone derivatives. Now these derivatives on condensation with Guanidine nitrate gives the vast range of phenyl pyrimidine amine Derivatives. Structure elucidation of synthesized compound had ...

  10. Experimental and Theoretical Investigations of Spectral, Tautomerism and Acid-Base Properties of Schiff Bases Derived from Some Amino Acids

    Energy Technology Data Exchange (ETDEWEB)

    Ebead, Y. H.; Salman, H. M. A.; Abdellah, M. A. [South Valley University, Qena (Egypt)

    2010-04-15

    The electronic absorption spectra of five Schiff bases derived from 2-hydroxy-1-naphthaldehyde with glycine, alanine, leucine, valine and phenylalanine have been measured in various solvents. The observed bands were assigned to the proper electronic transitions and compared with the predicted transitions at the semiempirical level of theory. The calculated equilibrium constants are in agreement with the experimental results, predicting the existence of all studied compounds predominantly or completely in keto-imine tautomerism. On the other hand, a correlation between ν (cm{sup -1}) (main frequency of each compound) and the well known solvent parameters E{sub T} (30), ε{sub r}, π* has been made. Furthermore, the acid dissociation constants, pK{sub a}, were determined by using three different spectrophotometric methods.

  11. Structure and physicochemical properties of thin film photosemiconductor cells based on porphine derivatives

    Science.gov (United States)

    Kazak, A. V.; Usol'tseva, N. V.; Smirnova, A. I.; Bodnarchuk, V. V.; Sul'yanov, S. N.; Yablonskii, S. V.

    2016-05-01

    Photosemiconductor thin films based on two organic porphine derivatives have been investigated. These compounds have different pendent groups; the film morphology, along with the specific fabrication technique, is determined to a great extent by these groups. The films have been fabricated by vacuum sputtering and using the Langmuir-Schaefer method. According to the atomic force microscopy (AFM) data, the Langmuir-Schaefer films are more homogeneous than the sputtered ones. It is shown that the sputtered films based on substituted porphine have a looser stacking than the initial analog. A spectroscopy study revealed a bathochromic shift of the Soret band in the Langmuir-Schaefer films-sputtered films series. This shift is explained by the increase in the concentration and size of molecular aggregates in sputtered films. It is shown that a polycrystalline C60 fullerene film deposited onto an amorphous substituted porphine layer improves the photoelectric characteristics of the latter. Both the time stability of the photodiode structure and its ampere‒watt sensitivity increase (by a factor of 10 in the transition regime). The steady-state current does not change. The effect of polarity reversal of the photovoltaic signal is observed in a planar C60‒substituted metalloporphine heterostructure, which is similar to the pyroelectric effect. The polarity reversal can be explained by the contribution of the trap charge and discharge current at the interface between the amorphous photosemiconductor and crystalline photosemiconductor to the resulting photoelectric current.

  12. Deriving Shape-Based Features for C. elegans Locomotion Using Dimensionality Reduction Methods.

    Science.gov (United States)

    Gyenes, Bertalan; Brown, André E X

    2016-01-01

    High-throughput analysis of animal behavior is increasingly common following the advances of recording technology, leading to large high-dimensional data sets. This dimensionality can sometimes be reduced while still retaining relevant information. In the case of the nematode worm Caenorhabditis elegans, more than 90% of the shape variance can be captured using just four principal components. However, it remains unclear if other methods can achieve a more compact representation or contribute further biological insight to worm locomotion. Here we take a data-driven approach to worm shape analysis using independent component analysis (ICA), non-negative matrix factorization (NMF), a cosine series, and jPCA (a dynamic variant of principal component analysis [PCA]) and confirm that the dimensionality of worm shape space is close to four. Projecting worm shapes onto the bases derived using each method gives interpretable features ranging from head movements to tail oscillation. We use these as a comparison method to find differences between the wild type N2 worms and various mutants. For example, we find that the neuropeptide mutant nlp-1(ok1469) has an exaggerated head movement suggesting a mode of action for the previously described increased turning rate. The different bases provide complementary views of worm behavior and we expect that closer examination of the time series of projected amplitudes will lead to new results in the future. PMID:27582697

  13. Deriving Shape-Based Features for C. elegans Locomotion Using Dimensionality Reduction Methods

    Science.gov (United States)

    Gyenes, Bertalan; Brown, André E. X.

    2016-01-01

    High-throughput analysis of animal behavior is increasingly common following the advances of recording technology, leading to large high-dimensional data sets. This dimensionality can sometimes be reduced while still retaining relevant information. In the case of the nematode worm Caenorhabditis elegans, more than 90% of the shape variance can be captured using just four principal components. However, it remains unclear if other methods can achieve a more compact representation or contribute further biological insight to worm locomotion. Here we take a data-driven approach to worm shape analysis using independent component analysis (ICA), non-negative matrix factorization (NMF), a cosine series, and jPCA (a dynamic variant of principal component analysis [PCA]) and confirm that the dimensionality of worm shape space is close to four. Projecting worm shapes onto the bases derived using each method gives interpretable features ranging from head movements to tail oscillation. We use these as a comparison method to find differences between the wild type N2 worms and various mutants. For example, we find that the neuropeptide mutant nlp-1(ok1469) has an exaggerated head movement suggesting a mode of action for the previously described increased turning rate. The different bases provide complementary views of worm behavior and we expect that closer examination of the time series of projected amplitudes will lead to new results in the future. PMID:27582697

  14. QSAR modeling for quinoxaline derivatives using genetic algorithm and simulated annealing based feature selection.

    Science.gov (United States)

    Ghosh, P; Bagchi, M C

    2009-01-01

    With a view to the rational design of selective quinoxaline derivatives, 2D and 3D-QSAR models have been developed for the prediction of anti-tubercular activities. Successful implementation of a predictive QSAR model largely depends on the selection of a preferred set of molecular descriptors that can signify the chemico-biological interaction. Genetic algorithm (GA) and simulated annealing (SA) are applied as variable selection methods for model development. 2D-QSAR modeling using GA or SA based partial least squares (GA-PLS and SA-PLS) methods identified some important topological and electrostatic descriptors as important factor for tubercular activity. Kohonen network and counter propagation artificial neural network (CP-ANN) considering GA and SA based feature selection methods have been applied for such QSAR modeling of Quinoxaline compounds. Out of a variable pool of 380 molecular descriptors, predictive QSAR models are developed for the training set and validated on the test set compounds and a comparative study of the relative effectiveness of linear and non-linear approaches has been investigated. Further analysis using 3D-QSAR technique identifies two models obtained by GA-PLS and SA-PLS methods leading to anti-tubercular activity prediction. The influences of steric and electrostatic field effects generated by the contribution plots are discussed. The results indicate that SA is a very effective variable selection approach for such 3D-QSAR modeling.

  15. Recent developments and future prospects on bio-based polyesters derived from renewable resources: A review.

    Science.gov (United States)

    Zia, Khalid Mahmood; Noreen, Aqdas; Zuber, Mohammad; Tabasum, Shazia; Mujahid, Mohammad

    2016-01-01

    A significantly growing interest is to design a new strategy for development of bio-polyesters from renewable resources due to limited fossil fuel reserves, rise of petrochemicals price and emission of green house gasses. Therefore, this review aims to present an overview on synthesis of biocompatible, biodegradable and cost effective polyesters from biomass and their prospective in different fields including packaging, coating, tissue engineering, drug delivery system and many more. Isosorbide, 2,4:3,5-di-O-methylene-d-mannitol, bicyclic diacetalyzed galactaric acid, 2,5-furandicarboxylic acid, citric, 2,3-O-methylene l-threitol, dimethyl 2,3-O-methylene l-threarate, betulin, dihydrocarvone, decalactone, pimaric acid, ricinoleic acid and sebacic acid, are some important monomers derived from biomass which are used for bio-based polyester manufacturing, consequently, replacing the petrochemical based polyesters. The last part of this review highlights some recent advances in polyester blends and composites in order to improve their properties for exceptional biomedical applications i.e. skin tissue engineering, guided bone regeneration, bone healing process, wound healing and wound acceleration. PMID:26492854

  16. Catalytic wet air oxidation of 2-chlorophenol over sewage sludge-derived carbon-based catalysts

    Energy Technology Data Exchange (ETDEWEB)

    Tu, Yuting [Institut de recherches sur la catalyse et l’environnement de Lyon (IRCELYON), CNRS – Université Claude Bernard Lyon 1, 2 Avenue Albert Einstein, 69626 Villeurbanne Cedex (France); School of Environmental Science and Engineering, Sun Yat-Sen University, Guangzhou 510275 (China); Xiong, Ya; Tian, Shuanghong [School of Environmental Science and Engineering, Sun Yat-Sen University, Guangzhou 510275 (China); Guangdong Provincial Key Laboratory of Environmental Pollution Control and Remediation Technology, Guangzhou 510275 (China); Kong, Lingjun [School of Environmental Science and Engineering, Sun Yat-Sen University, Guangzhou 510275 (China); Descorme, Claude, E-mail: claude.descorme@ircelyon.univ-lyon1.fr [Institut de recherches sur la catalyse et l’environnement de Lyon (IRCELYON), CNRS – Université Claude Bernard Lyon 1, 2 Avenue Albert Einstein, 69626 Villeurbanne Cedex (France)

    2014-07-15

    Highlights: • A sewage sludge derived carbon-supported iron oxide catalyst (FeSC) was prepared. • FeSC exhibited high catalytic activity in the wet air oxidation of 2-chlorophenol. • A strong correlation was observed between the 2-CP conversion, the iron leaching and the pH. • Using an acetate buffer, the iron leaching was suppressed while keeping some catalytic activity. • A simplified reaction pathway was proposed for the CWAO of 2-CP over the FeSC catalyst. - Abstract: A sewage sludge derived carbon-supported iron oxide catalyst (FeSC) was prepared and used in the Catalytic Wet Air Oxidation (CWAO) of 2-chlorophenol (2-CP). The catalysts were characterized in terms of elemental composition, surface area, pH{sub PZC}, XRD and SEM. The performances of the FeSC catalyst in the CWAO of 2-CP was assessed in a batch reactor operated at 120 °C under 0.9 MPa oxygen partial pressure. Complete decomposition of 2-CP was achieved within 5 h and 90% Total Organic Carbon (TOC) was removed after 24 h of reaction. Quite a straight correlation was observed between the 2-CP conversion, the amount of iron leached in solution and the pH of the reaction mixture at a given reaction time, indicating a strong predominance of the homogeneous catalysis contribution. The iron leaching could be efficiently prevented when the pH of the solution was maintained at values higher than 4.5, while the catalytic activity was only slightly reduced. Upon four successive batch CWAO experiments, using the same FeSC catalyst recovered by filtration after pH adjustment, only a very minor catalyst deactivation was observed. Finally, based on all the identified intermediates, a simplified reaction pathway was proposed for the CWAO of 2-CP over the FeSC catalyst.

  17. Large-scale derived flood frequency analysis based on continuous simulation

    Science.gov (United States)

    Dung Nguyen, Viet; Hundecha, Yeshewatesfa; Guse, Björn; Vorogushyn, Sergiy; Merz, Bruno

    2016-04-01

    There is an increasing need for spatially consistent flood risk assessments at the regional scale (several 100.000 km2), in particular in the insurance industry and for national risk reduction strategies. However, most large-scale flood risk assessments are composed of smaller-scale assessments and show spatial inconsistencies. To overcome this deficit, a large-scale flood model composed of a weather generator and catchments models was developed reflecting the spatially inherent heterogeneity. The weather generator is a multisite and multivariate stochastic model capable of generating synthetic meteorological fields (precipitation, temperature, etc.) at daily resolution for the regional scale. These fields respect the observed autocorrelation, spatial correlation and co-variance between the variables. They are used as input into catchment models. A long-term simulation of this combined system enables to derive very long discharge series at many catchment locations serving as a basic for spatially consistent flood risk estimates at the regional scale. This combined model was set up and validated for major river catchments in Germany. The weather generator was trained by 53-year observation data at 528 stations covering not only the complete Germany but also parts of France, Switzerland, Czech Republic and Australia with the aggregated spatial scale of 443,931 km2. 10.000 years of daily meteorological fields for the study area were generated. Likewise, rainfall-runoff simulations with SWIM were performed for the entire Elbe, Rhine, Weser, Donau and Ems catchments. The validation results illustrate a good performance of the combined system, as the simulated flood magnitudes and frequencies agree well with the observed flood data. Based on continuous simulation this model chain is then used to estimate flood quantiles for the whole Germany including upstream headwater catchments in neighbouring countries. This continuous large scale approach overcomes the several

  18. The correlation between effective renal plasma flow (ERPF) and glomerular filtration rate (GFR) with renal scintigraphy 99mTc-DTPA study

    Science.gov (United States)

    Ratnasari, D.; Nazir, F.; Toresano, L. O. H. Z.; Pawiro, S. A.; Soejoko, D. S.

    2016-03-01

    The prevalence of chronic renal diseases in Indonesia has an increasing annual trend, because it is frequently unrecognized and often co-exists with other disease. GFR and ERPF are parameters currently utilized to estimate renal function at routine renal scintigraphy 99m-Tc DTPA study. This study used 99m-Tc DTPA to measure GFR and ERPF. The purpose of this study was to find the correlation between ERPF and GFR, for ERPF analysis with Schlegel's method, and GFR analysis with Gate's method, as well as to find correction factor between both variables. Analysis of renal scintigraphy has been performed at Department of Nuclear Medicine Pertamina Center Hospital to thirty patient images acquired from 2014 to 2015 which were analyzed retrospectively data, using gamma camera dual head with counting method from renal scintigraphy 99m-Tc DTPA study. The calculation was executed by means of both display and manual calculation. Pearson's statistical analysis resulted on Positive Correlation for all data, with ERPF and GFR (display) showing Strongly Positive Correlation (r = 0.82; p- value < 0.05). Standard deviation was found to be 27.58 and 107.64 for GFR and ERPF (display), respectively. Our result indicated that the use of 99mTc-DTPA measure ERPF was not recommended.

  19. Preparation and preliminary biological evaluation of radiogallium-labeled DTPA-amlodipine complex for possible L-type calcium channel imaging

    International Nuclear Information System (INIS)

    A DTPA-conjugated amlodipine analog (DTPA-AMLO) 3, was prepared for possible voltage gated calcium channel imaging after radiolabeling with Ga-67. [67Ga]-DTPA-AMLO complex was prepared starting [67Ga]gallium chloride and DTPA-AMLO in 60-90 min at 50-60 C in phosphate buffer. The partition co-efficient and stability of the tracer was determined in final solution (25 C) and presence of human serum (37 C) up to 24 h. The biodistribution of the labeled compound in wild-type rats were determined up to 72 h using organ counting and SPECT. The radiolabled complex was prepared in high radiochemical purity (>96%, RTLC and >98% HPLC) and significant specific activity (7-10 GBq/mmol). The log P for the complex was calculated as -0.594, consistent with a water soluble complex. The tracer is mostly washed out through kidneys which were in full compliance with the amlodipine metabolism and imaging studies demonstrated the same behavior. The tracer uptake in organs with smooth muscles was observed in stomach, colon as well as intestine.

  20. Preparation and preliminary biological evaluation of radiogallium-labeled DTPA-amlodipine complex for possible L-type calcium channel imaging

    Energy Technology Data Exchange (ETDEWEB)

    Firuzyar, Tahereh; Shafiee-Ardestani, Mehdi; Khalaj, Ali [Tehran University of Medical Sciences, Tehran (Iran, Islamic Republic of). Faculty of Pharmacy; Jalilian, Amir R.; Fazaeli, Yousef; Aboudzadeh, Mohammad Reza [Nuclear Science and Technology Research Institute (NSTRI), Tehran (Iran, Islamic Republic of). Radiopharmacy Research Group

    2014-07-01

    A DTPA-conjugated amlodipine analog (DTPA-AMLO) 3, was prepared for possible voltage gated calcium channel imaging after radiolabeling with Ga-67. [{sup 67}Ga]-DTPA-AMLO complex was prepared starting [{sup 67}Ga]gallium chloride and DTPA-AMLO in 60-90 min at 50-60 C in phosphate buffer. The partition co-efficient and stability of the tracer was determined in final solution (25 C) and presence of human serum (37 C) up to 24 h. The biodistribution of the labeled compound in wild-type rats were determined up to 72 h using organ counting and SPECT. The radiolabled complex was prepared in high radiochemical purity (>96%, RTLC and >98% HPLC) and significant specific activity (7-10 GBq/mmol). The log P for the complex was calculated as -0.594, consistent with a water soluble complex. The tracer is mostly washed out through kidneys which were in full compliance with the amlodipine metabolism and imaging studies demonstrated the same behavior. The tracer uptake in organs with smooth muscles was observed in stomach, colon as well as intestine.

  1. Evaluation in vitro and in rats of161Tb-DTPA-octreotide, a somatostatin analogue with potential for intraoperative scanning and radiotherapy

    NARCIS (Netherlands)

    M. de Jong (Marcel); W.A.P. Breeman (Wouter); B.F. Bernard (Bert); E.J. Rolleman (Edgar); L.J. Hoflande (Leo); T.J. Visser (Ton); B. Setyono-Han (Buddy); W.H. Bakker (Willem); M. van der Pluijm (M.); E.P. Krenning (Eric)

    1995-01-01

    textabstractThe characteristics of terbium-161 diethylene triamine penta-acetic acid (DTPA) labelled octreotide with respect to specific binding to somatostatin (octreotide) receptors on rat brain cortex membranes, biological activity, uptake and excretion by isolated perfused rat livers and metabol

  2. Development of {sup 177}Lu-DTPA-SPIO conjugates for potential use as a dual contrast SPECT/MRI imaging agent

    Energy Technology Data Exchange (ETDEWEB)

    Shanehsazzadeh, Saeed; Yousefnia, Hassan [Nuclear Science and Technology Research Institute (NSTRI), Tehran (Iran, Islamic Republic of); Gruettner, Cordula [Micromod Partikeltechnologie GmbH, Rostock (Germany); and others

    2016-08-01

    This study describes the preparation, biodistribution of {sup 177}Lu-DTPA-SPIO after intravenous injection in rats. The chelator DTPA dianhydride was conjugated to SPIO NPs using a small modification of the well-known cyclic anhydride method. Conjugation was done at a 1:2 (SPIO:ccDTPA) molar ratio. Conjugation reaction was purified with Magnetic assorting column (MACs) using high gradient magnetic field following incubation, the radio labeled conjugate was checked using RTLC method for labeling and purity checked. The RTLC showed that labeling yield was above 99% after purification and the compound have good in-vitro stabilities until 48 h post injection in the presence of human serum. The biodistribution of {sup 177}Lu-DTPA-SPIO in rats showed dramatic uptake in the reticuloendothelial system (RES) and their clearance is so fast in other organs especially in the blood. In conclusion, due to high uptakes of this radiotracer in the liver and spleen and their fast clearance from other tissues, especially in blood, it is suggested that this radiotracer would be suitable for RES studies.

  3. Model based period analysis of absolute and relative survival with R: data preparation, model fitting and derivation of survival estimates.

    Science.gov (United States)

    Holleczek, Bernd; Brenner, Hermann

    2013-05-01

    Period analysis is increasingly employed in analyses of long-term survival of patients with chronic diseases such as cancer, as it derives more up-to-date survival estimates than traditional cohort based approaches. It has recently been extended with regression modelling using generalized linear models, which increases the precision of the survival estimates and enables to assess and account for effects of additional covariates. This paper provides a detailed presentation how model based period analysis may be used to derive population-based absolute and relative survival estimates using the freely available R language and statistical environment and already available R programs for period analysis. After an introduction of the underlying regression model and a description of the software tools we provide a step-by-step implementation of two regression models in R and illustrate how estimates and a test for trend over time in relative survival may be derived using data from a population based cancer registry. PMID:23116692

  4. Evaluation of 99Tcm-DTPA glomerular filtration rate by the Gates method in patients with type 2 diabetes

    International Nuclear Information System (INIS)

    Objective: To study the changes of GFR measured in different time intervals after the injection of 99Tcm-DTPA and to evaluate the clinical value of GFR in patients with type 2 diabetes. Methods: Eleven healthy volunteers (6 males, 5 females; mean age (61.45±7.90) years, age range: 47-79 years) and 56 patients with type 2 diabetes (31 males, 25 females; mean age (60.98±6.96) years, age range: 45-75 years) were recruited. 99Tcm-DTPA was used to measure GFR for all subjects. ROI was drawn at 2 and 3 min post-injection and GFR was calculated by Gates method. Two-sample t test and Pearson correlation analysis were performed with SPSS 15.0. Results: SCr ((84.90±14.38) μmol/L) was significantly correlated with GFR in patients with type 2 diabetes. There was negative correlation between SCr and GFR for both, left and right kidneys at 3 min post-injection (r=-0.652, -0.636, -0.470, all P≤0.001) and at 2 min post-injection (r=-0.599, -0.553, -0.529, all P<0.001). Total GFR, GFR for left kidney and GFR for right kidney at 3 min post-injection in diabetes group ((69.77 ± 11.00),(33.12 ± 5.74), (37.34 ± 9.81) ml/min) were lower than those in control group ((97.89±5.98), (46.60±4.91), (51.28±4.20) ml/min; t=-8.212, -7.233, -4.069, all P<0.001). Conclusions: To calculate the GFR of the patients with type 2 diabetes, 3 min post-injection is the optimal time. Measurement of GFR by 99Tcm-DTPA is useful in early diagnosis of diabetic nephropathy. (authors)

  5. Variability in soil micronutrients extracted by DTPA and Mehlich-3 at the plot scale in an acidic environment

    Science.gov (United States)

    Paz-Ferreiro, Jorge; Lado, Marcos; de Abreu, Cleide A.

    2014-05-01

    Land use practices affect soil properties and nutrient supply. Very limited data are available on micronutrient extractability in northwest Spain. The aim of this study was to analyse long-term effects of land use on the concentration, variability and spatial distribution of soil nutrients. To this end, neighboring forest and cultivated stands were compared. The study was carried out in an acid, rich in organic matter soil developed over sediments at the province of Lugo, northwestern of Spain. Adjacent plots with a surface of 100 m2 were marked on regular square grids with 2-m spacing. Fe, Mn, Zn and Cu were extracted both by Mehlich-3 and DTPA solutions and determined by ICP-MS. General soil chemical and physical properties were routinely analyzed. In arable land, microelement concentration ranges were as follows: Fe (100 and 135 mg kg-1), Mn (7.6 and 21.5 mg kg-1), Zn (0.6 and 3.7 mg kg-1), and Cu (0.2 and 0.7 mg kg-1). In forest land, these ranges were: Fe (62 and 309 mg kg-1), Mn (0.2 and 2.1 mg kg-1), Zn (0.2 and 2.9 mg kg-1), and Cu (0.1 and 0.2 mg kg-1). With the exception of Fe-DTPA, microelement concentrations extracted both with DTPA and Mehlich-3 were higher in the cultivated than in the forest stand. Coefficients of variation were higher for the microelement content of the soil under forest. Principal component analysis was performed to evaluate associations between extractable microelements and general physico-chemical properties. At the studied scale, nutrient management was the main factor affecting the agricultural site, whereas soil-plant interactions were probably responsible for the higher variation within the forest site. Patterns of spatial variability of the studied nutrients at the small plot scale also were assessed by geostatistical techniques. Results were discussed in the frame of sustainable land use and organic matter decline with conventional tillage and sustainable land use

  6. Design of two-channel filter bank using nature inspired optimization based fractional derivative constraints.

    Science.gov (United States)

    Kuldeep, B; Singh, V K; Kumar, A; Singh, G K

    2015-01-01

    In this article, a novel approach for 2-channel linear phase quadrature mirror filter (QMF) bank design based on a hybrid of gradient based optimization and optimization of fractional derivative constraints is introduced. For the purpose of this work, recently proposed nature inspired optimization techniques such as cuckoo search (CS), modified cuckoo search (MCS) and wind driven optimization (WDO) are explored for the design of QMF bank. 2-Channel QMF is also designed with particle swarm optimization (PSO) and artificial bee colony (ABC) nature inspired optimization techniques. The design problem is formulated in frequency domain as sum of L2 norm of error in passband, stopband and transition band at quadrature frequency. The contribution of this work is the novel hybrid combination of gradient based optimization (Lagrange multiplier method) and nature inspired optimization (CS, MCS, WDO, PSO and ABC) and its usage for optimizing the design problem. Performance of the proposed method is evaluated by passband error (ϕp), stopband error (ϕs), transition band error (ϕt), peak reconstruction error (PRE), stopband attenuation (As) and computational time. The design examples illustrate the ingenuity of the proposed method. Results are also compared with the other existing algorithms, and it was found that the proposed method gives best result in terms of peak reconstruction error and transition band error while it is comparable in terms of passband and stopband error. Results show that the proposed method is successful for both lower and higher order 2-channel QMF bank design. A comparative study of various nature inspired optimization techniques is also presented, and the study singles out CS as a best QMF optimization technique. PMID:25034647

  7. Silver ion recognition using potentiometric sensor based on recently synthesized isoquinoline-1,3-dione derivatives

    Directory of Open Access Journals (Sweden)

    AJAR KAMAL

    2012-08-01

    Full Text Available The four derivatives of isoquinoline-1,3-dione based on β-lactum (I-IV, have been explored as neutral ionophores for preparing poly(vinylchloride based polymeric membrane electrodes (PME selective to silver(I ions. The addition of sodium tetraphenylborate (NaTPB and dioctylsebacate (DOS as a plasticizer was found to improve the performance of ion selective electrodes. The best performance was obtained with PME-1 based on ionophore I having composition: ionophore (9.2 mg, PVC (100.1 mg, DOS (201.1 mg and NaTPB (1.5 mg in 5 mL tetrahydrofuran. The electrode response was linear with Nernstian slope of 58.44 mV/decade in the concentration range of 1.0 x 10-1 M to 5.0 x 10-6 M and detection limit of 5.83 x 10-6 M. It performs satisfactorily over wide pH range of 1.0-5.5. The proposed sensor can be used over a period of more than three months without any significant drift in potential and shows good selectivity to silver(I ion over a number of cations especially with no interference of mercury(II ions. Sharp end point was obtained when the sensor was used as an indicator electrode for the potentiometric titration of silver(I ions with chloride ions and therefore this electrode (PME-1 could be used for quantitative determination of silver(I ion in synthetic water, silver foil and dental amalgam samples.

  8. A fungal phylogeny based on 42 complete genomes derived from supertree and combined gene analysis

    Directory of Open Access Journals (Sweden)

    Stajich Jason E

    2006-11-01

    Full Text Available Abstract Background To date, most fungal phylogenies have been derived from single gene comparisons, or from concatenated alignments of a small number of genes. The increase in fungal genome sequencing presents an opportunity to reconstruct evolutionary events using entire genomes. As a tool for future comparative, phylogenomic and phylogenetic studies, we used both supertrees and concatenated alignments to infer relationships between 42 species of fungi for which complete genome sequences are available. Results A dataset of 345,829 genes was extracted from 42 publicly available fungal genomes. Supertree methods were employed to derive phylogenies from 4,805 single gene families. We found that the average consensus supertree method may suffer from long-branch attraction artifacts, while matrix representation with parsimony (MRP appears to be immune from these. A genome phylogeny was also reconstructed from a concatenated alignment of 153 universally distributed orthologs. Our MRP supertree and concatenated phylogeny are highly congruent. Within the Ascomycota, the sub-phyla Pezizomycotina and Saccharomycotina were resolved. Both phylogenies infer that the Leotiomycetes are the closest sister group to the Sordariomycetes. There is some ambiguity regarding the placement of Stagonospora nodurum, the sole member of the class Dothideomycetes present in the dataset. Within the Saccharomycotina, a monophyletic clade containing organisms that translate CTG as serine instead of leucine is evident. There is also strong support for two groups within the CTG clade, one containing the fully sexual species Candida lusitaniae, Candida guilliermondii and Debaryomyces hansenii, and the second group containing Candida albicans, Candida dubliniensis, Candida tropicalis, Candida parapsilosis and Lodderomyces elongisporus. The second major clade within the Saccharomycotina contains species whose genomes have undergone a whole genome duplication (WGD, and their close

  9. Navigation systems based on registration of endoscopic and CT-derived virtual images for bronchofiberoscopic procedures.

    Science.gov (United States)

    Turcza, Paweł; Duplaga, Mariusz

    2004-01-01

    Bronchofiberoscopy is an essential diagnostic procedure in patients with lung cancer. Sampling methods employed during endoscopy of the respiratory tract are performed with the aim of diagnosis confirmation and staging. Transbronchial needle aspiration may be used for evaluation of lymph nodes neighbouring with trachea and bronchi. Many efforts have been undertaken to increase the sensitivity of this procedure including the application of endobronchial ultrasonography. In recent years several research groups have proposed models of navigating systems to provide computer assistance during bronchoscopic interventions. Although they have used different techniques, their objective was the same - enabling tracking location and movement of bronchofiberoscope tip with reference to previously-acquired computed tomography (CT) images. Since a fiber-optic bronchoscope is a rather long and flexible device, determination of its tip location is not an easy task. The adoption of optical tracking methods used in neurosurgery or laparoscopic surgery to endoscopy of the tracheobronchial tree is usually not possible. Another obstacle is related to the fact that bronchofiberoscopes usually have only one operational channel. This feature considerably limits the feasibility of navigation systems based on the use of small electromagnetic sensing devices or USG probes. The sources of positioning errors in such systems are respiratory movements and the lack of external referential coordinate system associated with the tracheobronchial tree.A promising option for development of a bronchoscopic guidance system is the application of image registration algorithms. Such an approach encompasses registration of endoscopic images to views derived from advanced imaging methods, e.g. CT. In the first step, reconstruction of a three-dimensional, endoluminal views is performed. Next, the position of the virtual camera in a CT-derived virtual model is determined using a complex multi-level image

  10. Inulin Derivatives Obtained Via Enhanced Microwave Synthesis for Nucleic Acid Based Drug Delivery.

    Science.gov (United States)

    Sardo, Carla; Craparo, Emanuela Fabiola; Fiorica, Calogero; Giammona, Gaetano; Cavallaro, Gennara

    2015-01-01

    A new class of therapeutic agents with a high potential for the treatment of different socially relevant human diseases is represented by Nucleic Acid Based Drugs (NABD), including small interfering RNAs (siRNA), decoy oligodeoxynucleotides (decoy ODN) and antisense oligonucleotides (ASOs). Although NABD can be engineered to be specifically directed against virtually any target, their susceptibility to nuclease degradation and the difficulty of delivery into target tissues severely limit their use in clinical practice and require the development of an appropriate nanostructured delivery system. For delivery of NABD, Inulin (Inu), a natural, water soluble and biocompatible polysaccharide, was derivatized by Spermine (Spm), a flexible molecule with four amine groups that, having pKa values in the range between 8-11, is mainly in the protonated form at pH 7.4. The synthesis of related copolymers (Inu-Spm) was performed by a two step reaction, using a method termed Enhanced Microwave Synthesis (EMS) which has the advantage, compared to conventional microwave reaction, that high amount of energy can be applied to the reaction system, by administering microwave irradiation and simultaneously controlling the temperature in the reaction vessel with cooled air. The synthesized inulin derivatives were characterized by FT-IR spectra and (1)H-NMR. INU-Spm derivatives with a degree of derivatization of about 14 % mol/mol were obtained. These polycations were tested to evaluate their ability to form non covalent complexes with genetic material (polyplexes). Agarose gel retardation assays showed that the obtained copolymers are able to electrostatically interact with DNA duplex to form polyplexes at different c/p weight ratios. Moreover, light scattering studies, performed to analyze size and z-potential of polyplexes, evidenced that copolymers are able to interact with genetic material leading to the formation of nanoscaled systems. In addition, biocompatibility of polyplexes

  11. [Study on the characters of phytoplankton chlorophyll fluorescence excitation spectra based on fourth-derivative].

    Science.gov (United States)

    Lu, Lu; Su, Rong-Guo; Wang, Xiu-Lin; Zhu, Chen-Jian

    2007-11-01

    Chlorophyll fluorescence excitation spectra of six phytoplankton species, belonging to Bacillariophyta and Dinophyta, were dealt by fourth-derivative analysis with the Matlab program. The results show that between 350 nm and 550 nm six fluorescence peaks were found in the fourth-derivative spectra, which are representatives of non-pigments, chlorophylls and carotenoides respectively. The method makes Bacillariophyta and Dinophyta more distinguishable when the fourth-derivative spectra are compared with the chlorophyll fluorescence excitation spectra. It can be used not only to discriminate the two groups of algaes, but also to reduce the effect of noise. The fluorescence peaks in the fourth-derivative spectra are proved to be stable.

  12. Synthesis of Urea based Chalcone Derivatives and Evaluate its Biological Activity

    Directory of Open Access Journals (Sweden)

    Arpita Desai

    2016-05-01

    Full Text Available Chalcones have been the center of attraction for researchers from several decades due to nits innumerous therapeutic application, Efforts have been done in my research to synthesized chalcones and their derivatives that further reacts with various substituted aldehyde to give corresponding substituted chalcone derivatives. Now these derivatives on condensation with Guanidine nitrate gives the vast range of phenyl pyrimidine amine Derivatives. Structure elucidation of synthesized compound had been made on the basis of element analysis, 1H NMR Spectra studies. The microbial activity of the synthesized compounds has been studied against the species bacillus subtillis, staphylococcus aureus, Escherichia coli, and salmonella typhi.

  13. Pulmonary clearance of 99mTc-DTPA and 99mTc-albumin in rabbits with surfactant dysfunction and lung injury

    International Nuclear Information System (INIS)

    We measured the pulmonary clearance of inhaled 99mTc-DTPA and 99mTc-albumin in rabbits with surfacant dysfunction induced by dioctyl sodium sulphosuccinate and in rabbits with lung injury induced by oleic acid. After inhalation of 99mTc-albumin in ten animals, clearance of the tracer from the lungs was monitored for 90 min. The first 30 min was a control period. Dioctyl sodium sulphosuccinate was then administered in aerosol and after another 30 min oleic acid was injected intravenously. Ten other rabbits were given 99mTc-DTPA, and clearance was externally recorded for 60 min. Five animals inhaled detergent aerosol and five animals were given oleic acid intravenously after 30 min. Airway pressures, tidal volume, and arterial blood gases were measured before and after each intervention. The half-life of 99mTc-albumin in the lung was 442 ± 123 min during the control period, 363 ± 52 min after detergent administration, and 134 ± 18 min after oleic acid administration. The half-life of 99mTc-DTPA was 94 ± 16 min before and 10 ± 0.6 min after detergent administration and 75 ± 12 min before and 18 ± 1.8 min after oleic acid administration. Gas exchange was not affected by administration of dioctyl sodium sulphosuccinate but markedly impaired after injection of oleic acid. Compliance of the respiratory system remained unaffected by detergent but decreased after injection of oleic acid. The results indicate that the rate limiting factors for the alveolo-capillary transfer of 99mTc-albumin and 99mTc-DTPA are different. Surfactant dysfunction affects the transfer of 99mTc-DTPA but not 99mTc-albumin. (author)

  14. Differential renal function measured by 99Tcm-DTPA and 99Tcm-DMSA in a complete unilateral renal obstruction rat model.

    Science.gov (United States)

    van de Wiele, C; Everaert, K; van der Eecken, H; van Haelst, J P; Simons, M; Dierckx, R A

    1997-11-01

    We performed a prospective study to determine whether 99Tcm-DTPA differential renal function (DRF) in the case of acute obstruction in a unipapillary kidney rat model shows a similar pattern of results as 99Tcm-DMSA in a multipapillary kidney model, and to exclude recirculation or a block of tubular transport of DMSA which might explain the discrepancy reported by Kelleher et al. In 15 male and 4 female Wistar rats (weight 300-350 g), 99Tcm-DMSA renal uptake was measured 24 h before complete obstruction of the right ureter, which served as reference values. Twenty-four hours after obstruction, 99Tcm-DTPA (18.5 MBq) renography and 99Tcm-DMSA (111 MBq) non-depth-corrected renal uptake measurements were performed in the 15 male rats; in the 4 female rats, 740 MBq of 99Tcm-DMSA were injected and absolute, non-depth-corrected renal uptake measured at 24, 48 and 72 h. 99Tcm-DMSA DRF of the right kidney ranged from 48 to 55% (mu: 51%; sigma: 2%) before obstruction and from 14 to 35% (mu: 24%; sigma: 6%) after obstruction, whereas 99Tcm-DTPA DRF ranged from 16 to 30% (mu: 25%; sigma: 4%). No significant differences were found between DRF measured by 99Tcm-DMSA and 99Tcm-DTPA (P = 0.18), or between DRF of the obstructed kidney as measured by 99Tcm-DMSA at 24, 48 and 72 h (P > 0.2). Hypothetically, the discrepancy between our findings and those of Kelleher et al. may be due to intratubular pressure. In conclusion, the present findings and those of Kelleher et al. suggest the differences in DRF following complete unilateral renal obstruction, as determined by DTPA and DMSA, are probably species-specific. Furthermore, recirculation and block of tubular transport are unlikely to contribute significantly to differences in DRF as measured by the two radiopharmaceuticals.

  15. Using 99mTc-DTPA radioaerosol inhalation lung scan as compared with computed tomography to detect lung injury in blunt chest trauma

    International Nuclear Information System (INIS)

    Detection of pulmonary contusion in patients with blunt chest trauma is very important so as to commence therapy immediately to avoid irreversible damage. The purpose of our study was to evaluate the efficacy of technetium-99m diethylene triamine penta-acetic acid (99mTc-DTPA) aerosol inhalation lung scintigraphy in comparison with chest computed tomography (CT) in the diagnosis of pulmonary contusion at acute blunt chest trauma. Twenty-nine patients with isolated blunt chest trauma were referred to the emergency department of our hospital, and nine healthy people participated in this study. Sixteen patients who had pulmonary contusion on CT scans were referred to as group 1, and 13 patients who had normal CT scans as group 2. Nine healthy people comprised a control group. 99mTc-DTPA aerosol inhalation lung scintigraphy was performed on the first day in all patients. The mean half time (T1/2) and penetration index values of 99mTc-DTPA clearance were significantly lower in groups 1 and 2 compared with the control group. Among the three groups, there were no significant differences in arterial blood gas analysis except for PO2. The mean T1/2 value of 99mTc-DTPA clearance did correlate with PO2 values but not with pH, PCO2, or HCO3 values. 99mTc-DTPA radioaerosol inhalation lung imaging may serve as a useful adjunct and supportive method to chest CT scanning for detecting mild pulmonary contusion. (author)

  16. Gd-DTPA T1 relaxivity in brain tissue obtained by convection-enhanced delivery, magnetic resonance imaging and emission spectroscopy

    Energy Technology Data Exchange (ETDEWEB)

    Haar, Peter J [Department of Anatomy and Neurobiology, Virginia Commonwealth University, Richmond, VA (United States); Broaddus, William C; Chen Zhijian; Gillies, George T [Department of Neurosurgery, Virginia Commonwealth University, Richmond, VA (United States); Fatouros, Panos P; Corwin, Frank D, E-mail: wbroaddus@mcvh-vcu.ed [Department of Radiology, Virginia Commonwealth University, Richmond, VA (United States)

    2010-06-21

    A common approach to quantify gadolinium (Gd) contrast agents involves measuring the post-contrast change in T1 rate and then using the constant T1 relaxivity R to determine the contrast agent concentration. Because this method is fast and non-invasive, it could be potentially valuable in many areas of brain research. However, to accurately measure contrast agent concentrations in the brain, the T1 relaxivity R of the specific agent must be accurately known. Furthermore, the macromolecular content and compartmentalization of the brain extracellular space (ECS) are expected to significantly alter R from values measured in aqueous solutions. In this study, the T1 relaxivity R of gadolinium-diethylene-triamine penta-acetic acid (Gd-DTPA) was measured following direct interstitial infusions of three different contrast agent concentrations to the parenchyma of rat brains. Changes in magnetic resonance (MR) T1 values were compared to brain slice concentrations determined with inductively coupled plasma atomic emission spectroscopy (ICP-AES) to determine R in 15 rats. Additionally, samples of cerebrospinal fluid, blood and urine were analyzed to evaluate possible Gd-DTPA clearance from the brain. The T1 relaxivity R of Gd-DTPA in the brain ECS was measured to be 5.35 (mM s){sup -1} in a 2.4 T field. This value is considerably higher than estimations used in studies by other groups. Measurements of brain Gd-DTPA tissue concentrations using MRI and ICP-AES demonstrated a high degree of coincidence. Clearance of Gd-DTPA was minimal at the time point immediately after infusion. These results suggest that the environment of the brain does in fact significantly affect Gd T1 relaxivity, and that MRI can accurately measure contrast agent concentrations when this relaxivity is well characterized.

  17. The diagnostic value of Gd-EOB-DTPA-MRI for the diagnosis of focal nodular hyperplasia: a systematic review and meta-analysis

    Energy Technology Data Exchange (ETDEWEB)

    Suh, Chong Hyun [University of Ulsan College of Medicine, Asan Medical Center, Department of Radiology and Research Institute of Radiology, 88 Olympic-ro 43-gil, Songpa-gu, Seoul (Korea, Republic of); Kim, Kyung Won; Shin, Yong Moon; Kim, Pyo Nyun [Asan Liver Center, Asan Medical Center, University of Ulsan College of Medicine, Seoul (Korea, Republic of); Kim, Gene Young [University of Missouri, School of Medicine, Columbia (United States); Park, Seong Ho [University of Ulsan College of Medicine, Asan Medical Center, Department of Radiology and Research Institute of Radiology, 88 Olympic-ro 43-gil, Songpa-gu, Seoul (Korea, Republic of); Asan Liver Center, Asan Medical Center, University of Ulsan College of Medicine, Seoul (Korea, Republic of)

    2015-04-01

    We aimed to systematically review the gadoxetic acid-enhanced magnetic resonance imaging (Gd-EOB-DTPA-MRI) findings of focal nodular hyperplasia (FNH) and its diagnostic value. A thorough literature search was conducted in Ovid-MEDLINE and EMBASE databases to identify studies evaluating Gd-EOB-DTPA-MRI findings of FNH. To evaluate the frequency of characteristic imaging findings on Gd-EOB-DTPA-MRI, pooled proportions of high/iso signal intensity (SI) on the hepatobiliary phase (HBP), arterial enhancement, high/iso SI on the portal-venous phase (PVP) or equilibrium phase (EP), and the central scar were calculated. Meta-analysis was performed to evaluate the diagnostic accuracy of high/iso SI on HBP for distinguishing FNH from hepatocellular adenoma. A review of 96 articles identified ten eligible articles with 304 patients with FNHs for meta-analysis. Pooled proportion of the Gd-EOB-DTPA-MRI findings showed that high/iso SI on the HBP, arterial enhancement, and high/iso SI on the PVP/EP were observed in 93% (95% CI, 90-97%), 99% (95% CI, 97-100%), and 97% (95% CI, 95-99%) of FNHs, respectively, while a central scar was observed in 61% of FNHs (95% CI, 47-74%). High/iso SI on the HBP was highly accurate for distinguishing FNH from hepatocellular adenoma, with a summary sensitivity of 93.9% (95% CI, 89.1-97.1%) and a specificity of 95.3% (95% CI, 88.4-98.7%). High/iso SI on the HBP of Gd-EOB-DTPA-MRI is characteristic and a prevalent finding of FNHs and can be helpful in the management of patients with FNH. (orig.)

  18. Evaluation of methods to derive green-up dates based on daily NDVI satellite observations

    Science.gov (United States)

    Doktor, Daniel

    2010-05-01

    Bridging the gap between satellite derived green-up dates and in situ phenological observations has been the purpose of many studies over the last decades. Despite substantial advancements in satellite technology and data quality checks there is as yet no universally accepted method for extracting phenological metrics based on satellite derived vegetation indices. Dependent on the respective method derived green-up dates can vary up to serveral weeks using identical data sets. Consequently, it is difficult to compare various studies and to accurately determine an increased vegetation length due to changing temperature patterns as observed by ground phenological networks. Here, I compared how the characteristic NDVI increase over temperate deciduous forests in Germany in spring relates to respective budburst events observed on the ground. MODIS Terra daily surface reflectances with a 250 m resolution (2000-2008) were gathered to compute daily NDVI values. As ground truth, observations of the extensive phenological network of the German Weather Service were used. About 1500 observations per year and species (Beech, Oak and Birch) were available evenly distributed all over Germany. Two filtering methods were tested to reduce the noisy raw data. The first method only keeps NDVI values which are classified as ‚ideal global quality' and applies on those a temporal moving window where values are removed which differ more than 20% of the mean. The second method uses an adaptation of the BISE (Best Index Slope Extraction) algorithm. Subsequently, three functions were fitted to the selected observations: a simple linear interpolation, a sigmoidal function and a double logistic sigmoidal function allowing to approximate two temporally separated green-up signals. The green-up date was then determined at halfway between minimum and maximum (linear interpolation) or at the inflexion point of the sigmoidal curve. A number of global threshold values (NDVI 0.4,0.5,0.6) and

  19. New borehole-derived results on temperatures at the base of the Fennoscandian ice sheet

    Science.gov (United States)

    Rath, Volker; Vogt, Christian; Mottaghy, Darius; Kukkonen, Ilmo; Tarasov, Lev

    2014-05-01

    During the last few years, a data base of deep boreholes (>1000 m )in the area of the Fennoscandian ice sheet has been collected, including boreholes from Russia, Poland, Finland, Sweden and Norway. All of these are supposed to have recorded local basal ice conditions during the last glacial cycle. However, at each of these sites we are confronted with particular problems of interpretation. Here, we will concentrate on two very deep boreholes, namely the Outokumpu ICDP borehole (OKU, ≡2500 m) and a set of boreholes of intermediate depth (up to 1300 m) in the immediate meighborhood of the Kola superdeep borehole SG3. In the first case, OKU, we have developed a strategy combining the use of a traditional variational inversion of thye Tikhonov type, with a MCMC approach for the exploration of the associated uncertainty. A wide distribution around the result of the variational approach was chosen, with a time dependent temporal correlation length reflecting the loss of resolution back in time. The results fit very well with region independent results from different proxies, multi-proxy reconstructions, and instrumental data. They also are consistent with surface temperatures derived from recent calibrated ice sheet models. The SAT-GST offset independently derived from shallow borehole observations in the area was a crucial step to obtain theses results. The second case, SG3, has been studied a long time, and no final result was obtained regarding the question whether the observed heat flow density profile is caused by paleoclimate, fluid flow, or both. Earlier studies, as well as forward modelling using the results of the aforementioned ice sheet model indicate that paleoclimate alone can not explain the observations. We tested the model derived from the set of shallow boreholes against the temperature log from the main superdeep SG3, which, in contrast to these, transects the main high-permeability zone. The comparison led to a favorable results, and is also

  20. Synthesis and Biological Evaluation of Scutellaria Flavone Cyclaneaminol Mannich Base Derivatives as Novel CDK1 Inhibitors.

    Science.gov (United States)

    Ha, Lisha; Qian, Yuan; Zhang, Shixuan; Ju, Xiulan; Sun, Shiyou; Guo, Hongmin; Wang, Qianru; Li, Kangjian; Fan, Qingyu; Zheng, Yang; Li, Hailiang

    2016-01-01

    Cyclin-dependent kinase 1 (CDK1) is the only necessary CDK in the cell proliferation process and a new target in the research and development of anti-cancer drugs. Natural flavones are selective CDK1 inhibitors which can suppress the proliferation of cancer cells. However, their bioavailability is poor. To solve these problems, 6 Scutellaria flavones were isolated from hydrolyzed products of Scutellaria baicalensis and used as lead compounds, 18 Scutellaria flavones cyclane-aminol Mannich base derivatives were semi-synthesized and their biological activity as novel CDK1 inhibitors was evaluated. Results indicated that the biological activity of 8-Hydroxypiperidinemethyl-baicalein (BA-j) is the highest among these compounds. BA-j is a selective CDK1 inhibitor, and has broad-spectrum anti-proliferative activity in human cancer cells (IC50 12.3μM). BA-j can capture oxygen free radicals (.O2(-)) and selectively increase intracellular H2O2 level in cancer cells and activated lymphocytes, thus inducing their apoptosis rather than in normal cells. These findings suggest that BA-j selectively induces apoptosis in cancer and activated lymphocyte by controlling intracellular H2O2 level, and can be developed into a novel anti-proliferative agent for the treatment of cancer, AIDS, and some immune diseases. PMID:26411959

  1. Synthesis, Spectroscopic Characterization and Biological Activities of Transition Metal Complexes Derived from a Tridentate Schiff Base

    Directory of Open Access Journals (Sweden)

    J. Senthil Kumaran

    2013-01-01

    Full Text Available A new series of Cu (II, Ni (II, Co (II and Zn (II complexes have been synthesized from the Schiff base derived from 4-hydroxy-3-methoxybenzylidine-4-aminoantipyrine and 2-aminophenol. The structural features have been determined from their elemental analysis, magnetic susceptibility, molar conductance, Mass, IR, UV-Vis, 1H-NMR, 13C-NMR and ESR spectral studies. The redox behavior of the copper complex has been studied by cyclic voltammetry. The data confirm that the complexes have composition of ML2 type. The electronic absorption spectral data of the complexes propose an octahedral geometry around the central metal ion. All the metal complexes with DNA structure were guided by the presence of inter-molecular C–H⋯O and C–H⋯N hydrogen bonds. The biological activity of the synthesized compounds were tested against the bacterial species such as Bacillus subtilis, Staphylococcus aureus, Proteus vulgaris and fungal species such as Candida albicans by the well-diffusion method.

  2. Cadmium-sensitive electrode based on tetracetone derivatives of p-tert-butylcalix[8]arene

    Energy Technology Data Exchange (ETDEWEB)

    Dernane, C. [Université de Jijel, Laboratoire de Matériaux: Elaborations-Propriétés-Applications, BP 98, Ouled Aissa, 18000 Jijel (Algeria); Zazoua, A., E-mail: azazoua@yahoo.fr [Université de Jijel, Laboratoire de Matériaux: Elaborations-Propriétés-Applications, BP 98, Ouled Aissa, 18000 Jijel (Algeria); Kazane, I. [Université de Jijel, Laboratoire de Matériaux: Elaborations-Propriétés-Applications, BP 98, Ouled Aissa, 18000 Jijel (Algeria); Jaffrezic-Renault, N. [Université de Lyon, LSA-UMR 5180 CNRS, Université Claude Bernard Lyon 1, 69622 Villeurbanne cedex (France)

    2013-10-15

    The performance of a cadmium-sensitive electrode based on the tetracetone derivatives of p-tert butylcalix[8]arene was investigated. The ion-sensitivity of the calix[8]arene was examined via cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), X-ray photoelectron spectrometry, UV/Vis spectroscopy and FT-IR spectroscopy. The sensitive membrane containing the active ionophore was cast onto the surface of a gold electrode. The electrode exhibited a linear relationship between the charge transfer resistance (R{sub ct}) and the logarithm of the detected ion concentration. The cathodic peak at a potential of 0.56 V increased linearly as the Cd{sup 2+} ion concentration increased. The detection limit of the device reached 10{sup −7} M with high sensitivity toward cadmium. - Highlights: • The performances of cadmium-sensitive electrode were investigated. • The sensitive membrane was casted on the surface of a gold electrode. • The electrode showed a linear relationship between the R{sub ct} and the ion concentration. • The detection limit of the device was 10{sup −7} M with high sensitivity toward cadmium.

  3. Synthesis and Crystallographic Insight into the Structural Aspects of Some Novel Adamantane-Based Ester Derivatives.

    Science.gov (United States)

    Kumar, C S Chidan; Kwong, Huey Chong; Mah, Siau Hui; Chia, Tze Shyang; Loh, Wan-Sin; Quah, Ching Kheng; Lim, Gin Keat; Chandraju, Siddegowda; Fun, Hoong-Kun

    2015-01-01

    Adamantyl-based compounds are commercially important in the treatments for neurological conditions and type-2 diabetes, aside from their anti-viral abilities. Their values in drug design are chronicled as multi-dimensional. In the present study, a series of 2-(adamantan-1-yl)-2-oxoethyl benzoates, 2(a-q), and 2-(adamantan-1-yl)-2-oxoethyl 2-pyridinecarboxylate, 2r, were synthesized by reacting 1-adamantyl bromomethyl ketone with various carboxylic acids using potassium carbonate in dimethylformamide medium at room temperature. Three-dimensional structures studied using X-ray diffraction suggest that the adamantyl moiety can serve as an efficient building block to synthesize 2-oxopropyl benzoate derivatives with synclinal conformation with a looser-packed crystal packing system. Compounds 2a, 2b, 2f, 2g, 2i, 2j, 2m, 2n, 2o, 2q and 2r exhibit strong antioxidant activities in the hydrogen peroxide radical scavenging test. Furthermore, three compounds, 2p, 2q and 2r, show good anti-inflammatory activities in the evaluation of albumin denaturation. PMID:26501248

  4. Synthesis and Crystallographic Insight into the Structural Aspects of Some Novel Adamantane-Based Ester Derivatives

    Directory of Open Access Journals (Sweden)

    C. S. Chidan Kumar

    2015-10-01

    Full Text Available Adamantyl-based compounds are commercially important in the treatments for neurological conditions and type-2 diabetes, aside from their anti-viral abilities. Their values in drug design are chronicled as multi-dimensional. In the present study, a series of 2-(adamantan-1-yl-2-oxoethyl benzoates, 2(a–q, and 2-(adamantan-1-yl-2-oxoethyl 2-pyridinecarboxylate, 2r, were synthesized by reacting 1-adamantyl bromomethyl ketone with various carboxylic acids using potassium carbonate in dimethylformamide medium at room temperature. Three-dimensional structures studied using X-ray diffraction suggest that the adamantyl moiety can serve as an efficient building block to synthesize 2-oxopropyl benzoate derivatives with synclinal conformation with a looser-packed crystal packing system. Compounds 2a, 2b, 2f, 2g, 2i, 2j, 2m, 2n, 2o, 2q and 2r exhibit strong antioxidant activities in the hydrogen peroxide radical scavenging test. Furthermore, three compounds, 2p, 2q and 2r, show good anti-inflammatory activities in the evaluation of albumin denaturation.

  5. Dynamic Covalent Chemistry-based Sensing: Pyrenyl Derivatives of Phenylboronic Acid for Saccharide and Formaldehyde

    Science.gov (United States)

    Chang, Xingmao; Fan, Jiayun; Wang, Min; Wang, Zhaolong; Peng, Haonan; He, Gang; Fang, Yu

    2016-01-01

    We synthesized two specially designed pyrenyl (Py) derivatives of phenylboronic acid, PSNB1 and PSNB2, of which PSNB2 self-assemble to form dynamic aggregate in methanol-water mixture (1:99, v/v) via intermolecular H-bonding and pi-pi stacking. Interestingly, the dynamic aggregate shows smart response to presence of fructose (F) as evidenced by fluorescence color change from green to blue. More interestingly, the fluorescence emission of the resulted PSNB2-F changes from blue to green with the addition of formaldehyde (FA). The reason behind is formation of a PSNB2-F dimer via FA cross-linking. Based upon the reactions as found, sensitive and fast sensing of F and FA in water was realized, of which the experimental DLs could be significantly lower than 10 μM for both analytes, and the response times are less than 1 min. It is believed that not only the materials as created may have the potential to find real-life applications but also the strategy as developed can be adopted to develop other dynamic materials. PMID:27498703

  6. Green synthesis of novel quinoline based imidazole derivatives and evaluation of their antimicrobial activity

    Directory of Open Access Journals (Sweden)

    N.C. Desai

    2014-12-01

    Full Text Available We have described the conventional and microwave method for the synthesis of N-(4-((2-chloroquinolin-3-ylmethylene-5-oxo-2-phenyl-4,5-dihydro-1H-imidazol-1-yl(arylamides 3a–l. It is observed that the solvent-free microwave thermolysis is a convenient, rapid, high-yielding, and environmental friendly protocol for the synthesis of quinoline based imidazole derivatives when compared with conventional reaction in a solution phase. Antimicrobial activity of the newly synthesized compounds is screened in vitro on the following microbial cultures: Escherichia coli (MTCC 443, Pseudomonas aeruginosa (MTCC 1688, Staphylococcus aureus (MTCC 96, Streptococcus pyogenes (MTCC 442, Candida albicans (MTCC 227, Aspergillus niger (MTCC 282, Aspergillus clavatus (MTCC 1323. All the synthesized bio-active molecules are tested for their in vitro antimicrobial activity by bioassay namely serial broth dilution. Among these compounds 3c, 3d, 3f, 3h and 3j show significant potency against different microbial strains. All the compounds have been characterized by IR, 1H NMR, 13C NMR and mass spectral data. On the basis of statistical analysis, it is observed that these compounds give significant co-relation.

  7. Dynamic Covalent Chemistry-based Sensing: Pyrenyl Derivatives of Phenylboronic Acid for Saccharide and Formaldehyde

    Science.gov (United States)

    Chang, Xingmao; Fan, Jiayun; Wang, Min; Wang, Zhaolong; Peng, Haonan; He, Gang; Fang, Yu

    2016-08-01

    We synthesized two specially designed pyrenyl (Py) derivatives of phenylboronic acid, PSNB1 and PSNB2, of which PSNB2 self-assemble to form dynamic aggregate in methanol-water mixture (1:99, v/v) via intermolecular H-bonding and pi-pi stacking. Interestingly, the dynamic aggregate shows smart response to presence of fructose (F) as evidenced by fluorescence color change from green to blue. More interestingly, the fluorescence emission of the resulted PSNB2-F changes from blue to green with the addition of formaldehyde (FA). The reason behind is formation of a PSNB2-F dimer via FA cross-linking. Based upon the reactions as found, sensitive and fast sensing of F and FA in water was realized, of which the experimental DLs could be significantly lower than 10 μM for both analytes, and the response times are less than 1 min. It is believed that not only the materials as created may have the potential to find real-life applications but also the strategy as developed can be adopted to develop other dynamic materials.

  8. Derivation of an observation-based map of North African dust emission

    Energy Technology Data Exchange (ETDEWEB)

    Evan, Amato T.; Fiedler, Stephanie; Zhao, Chun; Menut, Laurent; Schepanski, Kerstin; Flamant, C.; Doherty, Owen

    2015-03-01

    Changes in the emission, transport and deposition of aeolian dust have profound effects on regional climate, so that characterizing the lifecycle of dust in observations and improving the representation of dust in global climate models is necessary. A fundamental aspect of characterizing the dust cycle is quantifying surface dust fluxes, yet no spatially explicit estimates of this flux exist for the World’s major source regions. Here we present a novel technique for creating a map of the annual mean emitted dust flux for North Africa based on retrievals of dust storm frequency from the Meteosat Second Generation Spinning Enhanced Visible and InfraRed Imager (SEVIRI) and the relationship between dust storm frequency and emitted mass flux derived from the output of five models that simulate dust. Our results suggest that 64 (±16)% of all dust emitted from North Africa is from the Bodélé depression, and that 13 (±3)% of the North African dust flux is from a depression lying in the lee of the Aïr and Hoggar Mountains, making this area the second most important region of emission within North Africa.

  9. Satellite-Based Derivation of High-Resolution Forest Information Layers for Operational Forest Management

    Directory of Open Access Journals (Sweden)

    Johannes Stoffels

    2015-06-01

    Full Text Available A key factor for operational forest management and forest monitoring is the availability of up-to-date spatial information on the state of forest resources. Earth observation can provide valuable contributions to these information needs. The German federal state of Rhineland-Palatinate transferred its inherited forest information system to a new architecture that is better able to serve the needs of centralized inventory and planning services, down to the level of forest districts. During this process, a spatially adaptive classification approach was developed to derive high-resolution forest information layers (e.g., forest type, tree species distribution, development stages based on multi-temporal satellite data. This study covers the application of the developed approach to a regional scale (federal state level and the further adaptation of the design to meet the information needs of the state forest service. The results confirm that the operational requirements for mapping accuracy can, in principle, be fulfilled. However, the state-wide mapping experiment also revealed that the ability to meet the required level of accuracy is largely dependent on the availability of satellite observations within the optimum phenological time-windows.

  10. Intramolecular photoinduced electron transfer of fluorescent probes based on 1,8-naphthalimide and aniline derivatives

    Science.gov (United States)

    Burmistrova, Natalia A.; Mushtakova, Svetlana P.; Zilberg, Rufina A.; Vakulin, Ivan V.; Duerkop, Axel

    2015-03-01

    The effect of conformation and electronic structure of fluorescent probes based on 1,8-naphthalimide and aniline derivatives (4-methoxyaniline and N,N-dimethyl-p-phenylenediamine) on the intramolecular photoinduced electron transfer (PET) was investigated by density functional theory calculations (B3LYP/6-31G (d, p)). We established restricted rotation around spacer bonds of the model compounds and their protonated and oxidized forms do not block the convergence of the nitrogen atoms involved in the electron transfer at a distance of ~3Å, which is adequately for PET. Computed values of protonation free energy for the gas-phase (ΔG298 r) show that the investigated fluorescent probes are predominantly protonated on the nitrogen atoms of the donor moiety. Electron population and localization of the frontier orbitals (LUMO, HOMO, HOMO-1) on the donor and acceptor moieties are transformed under protonation and one-electron oxidation of fluorescent probes. The results show that appearance or disappearance of the PET can be predicted by the energy difference between the frontier orbitals and the nature of their location of donor and acceptor moieties, which is in agreement with the PET theory and observed experimental data.

  11. Physical and electrochemical characteristics of supercapacitors based on carbide derived carbon electrodes in aqueous electrolytes

    Science.gov (United States)

    Eskusson, Jaanus; Jänes, Alar; Kikas, Arvo; Matisen, Leonard; Lust, Enn

    FIB-SEM, XPS and gas adsorption methods have been used for the characterisation of physical properties of microporous carbide derived carbon electrodes prepared from Mo 2C at 600 °C (noted as CDC-Mo 2C). Cyclic voltammetry, constant current charge/discharge, and electrochemical impedance spectroscopy have been applied to establish the electrochemical characteristics for supercapacitors consisting of the 1 M Na 2SO 4, KOH, tetraethyl ammonium iodide or 6 M KOH aqueous electrolyte and CDC-Mo 2C electrodes. The N 2 sorption values obtained have been correlated with electrochemical characteristics for supercapacitors in various aqueous electrolytes. The maximum gravimetric energy, E max, and gravimetric power, P max, for supercapacitors (taking into consideration the active material weight) have been obtained at cell voltage 0.9 V for 6 M KOH aqueous supercapacitor (E max = 5.7 Wh kg -1 and P max = 43 kW kg -1). For 1 M TEAI based SC somewhat higher E max (6.2 Wh kg -1) and comparatively low P max (7.0 kW kg -1) have been calculated.

  12. Diagnosis of pulmonary infections with HIV (+) patients. Brought of aerosol DTPA-Tc99m and of Ga67 citrate

    International Nuclear Information System (INIS)

    The pulmonary clearance of aerosol DTPA-Tc99m is a technology easy to use, well support by patients with immediate results. With the pneumonia at Pneumocystis Carinii (PPC), the clearance is more sensitive and more specific than the thoracic scintigraphy with Ga67. Used with a thorax radiography, results will lead to three directions: High probability of PPC, di phasic curve and very fast T50, equal inferior to 5,10 mn. Low probability of PPC, monoexponential curve and abnormal thorax radiography or di phasic curve with a value T50 superior to 5,10 mn. With these patients other explorations will be made (Ga67, biopsy) and if possible search pulmonary tuberculosis. Extra pulmonary pathology: monoexponential curve associated with a normal thoracic radiography. 2 figs

  13. Schistosomiasis haematobia in Brazilian patients: clinical and renal functional evaluation with {sup 99m}Tc-DTPA

    Energy Technology Data Exchange (ETDEWEB)

    DAlmeida, J.; Maliska, C.I.; Pellegrini, P.M.; Collares, R.A.I [Army Central Hospital, Rio de Janeiro, RJ (Brazil). Nuclear Medicine Service]. E-mail: joaquimjdalmeida@yahoo.com.br; Penas, M.E.; Cruz, M.G.A. [Clementino Fraga Filho School Hospital, (UFRJ), Rio de Janeiro, RJ (Brazil). Nuclear Medicine Service

    2007-07-01

    The present study was carried out at the Army Central Hospital, Rio de Janeiro, Brazil, from September 2000 to December 2001, employing diethylenetriamine penta-acetic acid labeled with technetium-99m ({sup 99m}Tc-DTPA) to evaluate the renal function of nineteen symptomatic patients infected with S. haematobium during a peace mission in Mozambique. Results evidenced that the most frequent clinical manifestations were hematuria (68.4%) and low back pain (68.4%) and 73.7% patients had altered dynamic renal scintigraphy expressed by an increase in the excretory phase independently of the symptoms duration; furthermore, none of them had mechanical obstructive pattern. Schistosoma haematobium glomerulopathy could be considered a pathological finding without correlation with the disease clinical manifestations. (author)

  14. Study of the renal function in diabetic patients by 99sup(m)Tc-DTPA and 131I-Hippuran

    International Nuclear Information System (INIS)

    Twenty-six patients with diabetes in different stages were studied by radioisotopic techniques in an attempt to detect early alterations of renal function. Renal scintillography, 99sup(m)Tc-DTPA glomerular filtration rate, renogram and 131I-Hippuran effective renal plasma flow were the techniques employed. Renal evaluation was filled out by biochemical analysis of plasmatic urea and creatinine, 24 h-proteinuria and funduscopy of patients. The renal disease of diabetic patient is a result of the sum of many factors and subclinical functional alterations that appear can be ascribed to reversible hemodynamic alterations, such as decrease of plasma flow with increase of ultrafiltrated plasma fraction and of glomerular filtration. Mechanisms that lead to these alterations are not yet stablished. This work suggests that the diabetic patients well metabolic compensated present less incidence of degenerative complications, among them renal lesions. (Author)

  15. Capillary transfer constant of Gd-DTPA in the myocardium at rest and during vasodilation assessed by MRI

    DEFF Research Database (Denmark)

    Fritz-Hansen, T; Rostrup, Egill; Søndergaard, Lise;

    1998-01-01

    The purpose of this study was to determine whether the capillary transfer constant (Ki) of gadolinium-DTPA was sensitive to perfusion changes and whether ischemic regions in the myocardium could be identified using the modified Kety formula. Ki was measured at rest and during dipyridamole......-induced vasodilation in 10 healthy volunteers and in 10 patients with ischemic heart disease. Ki increased by a factor of 2.5+/-1.2 (mean +/- SD) from 55+/-16 ml 100 g(-1)min(-1) at rest to 136+/-46 ml 100 g(-1)min(-1) (P ... during vasodilation in ischemic regions (50+/-18 versus 49+/-30 ml 100 g(-1)min(-1) (P > 0.4)). Ki increased in nonischemic regions by a factor of 2.0+/-0.8 from 44+/-17 to 81+/-32 ml 100 g(-1)min(-1) during vasodilation (P transfer constant is sensitive...

  16. Enhanced efficacy of combined {sup 213}Bi-DTPA-F3 and paclitaxel therapy of peritoneal carcinomatosis is mediated by enhanced induction of apoptosis and G2/M phase arrest

    Energy Technology Data Exchange (ETDEWEB)

    Vallon, Mario; Seidl, Christof; Blechert, Birgit; Li, Zhoulei; Gaertner, Florian C.; Senekowitsch-Schmidtke, Reingard; Essler, Markus [Technische Universitaet Muenchen, Department of Nuclear Medicine, Munich (Germany); Gilbertz, Klaus-Peter [German Armed Forces, Institute of Radiobiology, Munich (Germany); Baumgart, Anja [Technische Universitaet Muenchen, III. Medical Department, Munich (Germany); Aichler, Michaela; Feuchtinger, Annette; Walch, Axel K. [Helmholtz Zentrum Muenchen, Institute of Pathology, Neuherberg (Germany); Bruchertseifer, Frank; Morgenstern, Alfred [Institute for Transuranium Elements, European Commission, Joint Research Centre, Karlsruhe (Germany)

    2012-12-15

    Targeted therapy with {alpha}-particle emitting radionuclides is a promising new option in cancer therapy. Stable conjugates of the vascular tumour-homing peptide F3 with the {alpha}-emitter {sup 213}Bi specifically target tumour cells. The aim of our study was to determine efficacy of combined {sup 213}Bi-diethylenetriaminepentaacetic acid (DTPA)-F3 and paclitaxel treatment compared to treatment with either {sup 213}Bi-DTPA-F3 or paclitaxel both in vitro and in vivo. Cytotoxicity of treatment with {sup 213}Bi-DTPA-F3 and paclitaxel, alone or in combination, was assayed towards OVCAR-3 cells using the alamarBlue assay, the clonogenic assay and flow cytometric analyses of the mode of cell death and cell cycle arrest. Therapeutic efficacy of the different treatment options was assayed after repeated treatment of mice bearing intraperitoneal OVCAR-3 xenograft tumours. Therapy monitoring was performed by bioluminescence imaging and histopathologic analysis. Treatment of OVCAR-3 cells in vitro with combined {sup 213}Bi-DTPA-F3 and paclitaxel resulted in enhanced cytotoxicity, induction of apoptosis and G2/M phase arrest compared to treatment with either {sup 213}Bi-DTPA-F3 or paclitaxel. Accordingly, i.p. xenograft OVCAR-3 tumours showed the best response following repeated (six times) combined therapy with {sup 213}Bi-DTPA-F3 (1.85 MBq) and paclitaxel (120 {mu}g) as demonstrated by bioluminescence imaging and histopathologic investigation of tumour spread on the mesentery of the small and large intestine. Moreover, mean survival of xenograft mice that received combined therapy with {sup 213}Bi-DTPA-F3 and paclitaxel was significantly superior to mice treated with either {sup 213}Bi-DTPA-F3 or paclitaxel alone. Combined treatment with {sup 213}Bi-DTPA-F3 and paclitaxel significantly increased mean survival of mice with peritoneal carcinomatosis of ovarian origin, thus favouring future therapeutic application. (orig.)

  17. Molecular markers derived from bombesin for tumor diagnosis by SPECT and PET

    International Nuclear Information System (INIS)

    A high number of molecules have already been identified to have high affinity to some receptors overexpressed on tumour cells and the radiolabelling of those molecules offers the possibility of new compounds for tumour diagnosis and therapy by nuclear medicine. Among of those molecules, bombesin (BBN) has become focus of interest, as its BB2 receptors are known to be overexpressed in prostate, breast, colon, pancreatic and lung tumour, as long as glioblastomas and neuroblastomas. BBN agonists and antagonists have already been described for this purpose and promising results were obtained in preclinical studies. However, most of them exhibited high abdominal accumulation, especially in pancreas and intestines, which can compromise diagnosis accuracy and cause serious adverse effects in therapy. In this context, the goal of the present work to radiolabel new BBN derivatives with 111In and 68Ga and to evaluate their potential for BB2 positive tumors diagnosis by single photon emission tomography (SPECT) and positron emission tomography (PET). The structure of studied peptides was Q-YGn-BBN(6-14), where Q is the chelator, n is the number of glycine aminoacids in the spacer YGn and BBN(6-14) is the original bombesin sequence from the aminoacid 6 to 14. The derivative in which the last aminoacid (methionine, Met) was replaced by norleucine (Nle) was also evaluated. The experimental evaluation of the bombesin derivatives was divided into four steps: computational studies, molecular markers for SPECT, molecular markers for PET and toxicological studies. The theoretical partition (log P) and distribution (log D) coefficients were calculated for all bombesin derivatives conjugated to DTPA (diethylenetriaminepentaacetic acid) and DOTA (1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid) chelators applying computational programmes. Bombesin derivatives for SPECT were developed by radiolabelling DTPA-conjugated bombesin derivatives with 111In to determine the best spacer

  18. Solution properties and emulsification properties of amino acid-based gemini surfactants derived from cysteine.

    Science.gov (United States)

    Yoshimura, Tomokazu; Sakato, Ayako; Esumi, Kunio

    2013-01-01

    Amino acid-based anionic gemini surfactants (2C(n)diCys, where n represents an alkyl chain with a length of 10, 12, or 14 carbons and "di" and "Cys" indicate adipoyl and cysteine, respectively) were synthesized using the amino acid cysteine. Biodegradability, equilibrium surface tension, and dynamic light scattering were used to characterize the properties of gemini surfactants. Additionally, the effects of alkyl chain length, number of chains, and structure on these properties were evaluated by comparing previously reported gemini surfactants derived from cystine (2C(n)Cys) and monomeric surfactants (C(n)Cys). 2C(n)diCys shows relatively higher biodegradability than does C(n)Cys and previously reported sugar-based gemini surfactants. Both critical micelle concentration (CMC) and surface tension decrease when alkyl chain length is increased from 10 to 12, while a further increase in chain length to 14 results in increased CMC and surface tension. This indicates that long-chain gemini surfactants have a decreased aggregation tendency due to the steric hindrance of the bulky spacer as well as premicelle formation at concentrations below the CMC and are poorly packed at the air/water interface. Formation of micelles (measuring 2 to 5 nm in solution) from 2C(n)diCys shows no dependence on alkyl chain length. Further, shaking the mixtures of aqueous 2C(n)diCys surfactant solutions and squalane results in the formation of oil-in-water type emulsions. The highly stable emulsions are formed using 2C₁₂diCys or 2C₁₄diCys solution and squalane in a 1:1 or 2:1 volume ratio.

  19. Deriving hydraulic roughness from camera-based high resolution topography in field and laboratory experiments

    Science.gov (United States)

    Kaiser, Andreas; Neugirg, Fabian; Ebert, Louisa; Haas, Florian; Schmidt, Jürgen; Becht, Michael; Schindewolf, Marcus

    2016-04-01

    The hydraulic roughness, represented by Manning's n, is an essential input parameter in physically based soil erosion modeling. In order to acquire the roughness values for certain areas, on-site flow experiments have to be carried out. These results are influenced by the selection of the location of the test plot and are thereby based on the subjectiveness of the researchers. The study aims on the methodological development to acquire Manning's n by creating very high-resolution surface models with structure-from-motion approaches. Data acquisition took place during several field experiments in the Lainbach valley, southern Germany, and on agricultural sites in Saxony, eastern Germany, and in central Brazil. Rill and interrill conditions were simulated by flow experiments. In order to validate our findings stream velocity as an input for the manning equation was measured with coloured dye. Grain and aggregate sizes were derived by measuring distances from a best fit line to the reconstructed soil surface. Several diameters from D50 to D90 were tested with D90 showing best correlation between tracer experiments and photogrammetrically acquired data. A variety of roughness parameters were tested (standard deviation, random roughness, Garbrecht's n and D90). Best agreement in between the particle size and the hydraulic roughness was achieved with a non-linear sigmoid function and D90 rather than with the Garbrecht equation or statistical parameters. To consolidate these findings a laboratory setup was created to reproduce field data under controlled conditions, excluding unknown influences like infiltration and changes in surface morphology by erosion.

  20. In vivo Gd-DTPA concentration for MR lung perfusion measurements: Assessment with computed tomography in a porcine model

    Energy Technology Data Exchange (ETDEWEB)

    Puderbach, Michael [German Cancer Research Center, Department of Radiology, Heidelberg (Germany); Deutsches Krebsforschungszentrum, Department of Radiology (E010), Heidelberg (Germany); Risse, Frank; Semmler, Wolfhard [German Cancer Research Center, Department of Medical Physics in Radiology, Heidelberg (Germany); Biederer, Juergen [University Hospital Schleswig-Holstein, Department of Diagnostic Radiology, Campus Kiel (Germany); Ley-Zaporozhan, Julia; Ley, Sebastian [German Cancer Research Center, Department of Radiology, Heidelberg (Germany); University Heidelberg, Department of Pediatric Radiology, Heidelberg (Germany); Szabo, Gabor [University Heidelberg, Department of Cardiac Surgery, Heidelberg (Germany); Kauczor, Hans-Ulrich [University of Heidelberg, Department of Radiology, Heidelberg (Germany)

    2008-10-15

    A linear relationship between MR signal and contrast-agent concentration (CAC) of the arterial-input function (AIF) is crucial for MR lung-perfusion quantification. The aim was to determine the in-vivo real maximum CAC of the AIF, using cine CT measurements in a porcine model. A dilution series (Gd-DTPA, 0-20 mM) was examined by clinical time-resolved 3D-GRE MRI and by MDCT in cine CT mode. Using the CT setup, data were acquired in five pigs immediately after the injection of 0.05 mmol and 0.07 mmol/kg BW Gd-DTPA. For phantom measurements, mean signal values were determined using a region-of-interest (ROI) analysis and for animal measurements, a ROI was placed in the pulmonary trunk of the cine CT perfusion data sets. The CT phantom measurements were used to calculate the in-vivo maximum CAC corresponding to the HU values obtained in the pulmonary trunk by the cine CT study. Linearity of the AIF of the CT perfusion measurements was verified using the MR phantom measurement results. MR phantom measurements demonstrated linearity for concentrations of 0-4 mM. CT phantom measurements showed linear relation for the entire CAC range. Comparing in-vivo and in-vitro measurements, three of five CA injections at 0.05 mmol/kg and all 0.07 mmol/kg injections exceeded the range of linearity in MRI. The CA dose for quantification of lung perfusion with time-resolved MR studies must be chosen carefully since even with low doses (0.05 mmol/kg) the CAC may exceed the range of linearity in the AIF. (orig.)

  1. Assessment of pulmonary aerosol deposition and epithelial permeability in [sup 99m]Tc-DTPA inhalation scintigram

    Energy Technology Data Exchange (ETDEWEB)

    Kanazawa, Minoru; Suzuki, Yukio; Ishizaka, Akitoshi; Hasegawa, Naoki; Fujishima, Seitaro; Kawashiro, Takeo; Yokoyama, Tetsuro; Kubo, Atsushi; Hashimoto, Shozo (Keio Univ., Tokyo (Japan). School of Medicine)

    1993-05-01

    The degree of lung injury in bronchiolo-alveolar lesions may be quantitated from the pulmonary epithelial permeability estimated by [sup 99m]Tc-diethylene triamine penta acetate (DTPA) aerosol inhalation scintigram. However, significant aerosol deposition sometimes occurs in the central airways and obscures the peameability change in the lung periphery. The radioaerosol deposition pattern and its effct on assessing the pulmonary epithelial permeability was studied. [sup 99m]Tc-DTPA aerosol scintigraphy was performed in 47 patients with pulmonary fibrosis (PF), 12 patients with chronic obstructive pulmonary diseases (COPD), and 27 non-smoking and 17 smoking healthy volunteers. The scintigraphic images of the lungs were classified into 4 grades: 0=homogeneous distribution; 1=patchy distribution; 2=hot spots with partial defects; and 3= hot spots with little deposition in the lung field. The rate constant was used as a parameter for the permeability. The smokers and patients with PF showed increased kep values of 2.36[+-]1.21%/min (mean[+-]SD) and 2.49[+-]1.29% min as compared with the nonsmokers with 0.94[+-]0.27% min, respectively. The nonsmokers, smokers and 36 patients with PF were classified as deposition grade 0 or 1, suggesting good aerosol penetration to the lung periphery. All patients with COPD showed either grade 2 or 3 deposition. Aerosol deposition in the central airways can cause underestimation of the permeability because of the thicker lining layer in the bronchus than in the alveolus. In conclusion, the aerosol deposition pattern should be analyzed when the method is applied clinically to assess the permeability of the bronchiolo-alveolar epithelium. (author).

  2. Clearance of inhaled technetium-99m-DTPA as a clinical index of pulmonary vascular disease in systemic sclerosis

    Energy Technology Data Exchange (ETDEWEB)

    Kon, O.M.; Daniil, Z.; Bois, R.M. du [Royal Brompton Hospital, Interstitial Lung Disease Unit, London (United Kingdom); Black, C.M. [Royal Free Hospital, Dept. of Rheumatology, London (United Kingdom)

    1999-01-01

    This study evaluated the utility of the clearance time of inhaled diethylenetriamine pentaacetate (DTPA) to distinguish pulmonary vascular disease from early fibrosing alveolitis (FA) in patients with systemic sclerosis (SSc) It was hypothesized that this would be preserved in patients with vascular disease compared with FA, despite similar gas-transfer deficits and matching lung volumes, because of the preservation of alveolar epithelial integrity. All patients had SSc and were categorized into a control group (C; n=9), pulmonary vascular group (VAS; n=14) or FA group (n=14) dependent on the appearance on a computed tomography (CT) scan and the transfer factor of the lung for carbon monoxide (TL,CO) (VAS and FA {<=}70%, C {>=}80%). All patients had a forced vital capacity (FVC) of >80%. The TL,CO (median) was similar in the VAS (57.5%) and FA (60%) groups. There was a significant difference in median DTPA clearance half-times between FA (21.25 min) and VAS (46.5 min) (p=0.014) and between FA and C (84.5 min) (p=0.0004). No difference was found between VAS and C (p=0.0778). Follow-up data from the VAS group showed no subsequent development of FA on the CT scan and no decrease in FVC (n=13, mean 42 months). These results suggest that clearance of diethylenetriamine pentaacetate is preserved in patients likely to have pulmonary vascular disease and may be useful in distinguishing fibrosing alveolitis from vascular disease in systemic sclerosis. (au) 22 refs.

  3. Clearance of inhaled technetium-99m-DTPA as a clinical index of pulmonary vascular disease in systemic sclerosis

    International Nuclear Information System (INIS)

    This study evaluated the utility of the clearance time of inhaled diethylenetriamine pentaacetate (DTPA) to distinguish pulmonary vascular disease from early fibrosing alveolitis (FA) in patients with systemic sclerosis (SSc) It was hypothesized that this would be preserved in patients with vascular disease compared with FA, despite similar gas-transfer deficits and matching lung volumes, because of the preservation of alveolar epithelial integrity. All patients had SSc and were categorized into a control group (C; n=9), pulmonary vascular group (VAS; n=14) or FA group (n=14) dependent on the appearance on a computed tomography (CT) scan and the transfer factor of the lung for carbon monoxide (TL,CO) (VAS and FA ≤70%, C ≥80%). All patients had a forced vital capacity (FVC) of >80%. The TL,CO (median) was similar in the VAS (57.5%) and FA (60%) groups. There was a significant difference in median DTPA clearance half-times between FA (21.25 min) and VAS (46.5 min) (p=0.014) and between FA and C (84.5 min) (p=0.0004). No difference was found between VAS and C (p=0.0778). Follow-up data from the VAS group showed no subsequent development of FA on the CT scan and no decrease in FVC (n=13, mean 42 months). These results suggest that clearance of diethylenetriamine pentaacetate is preserved in patients likely to have pulmonary vascular disease and may be useful in distinguishing fibrosing alveolitis from vascular disease in systemic sclerosis. (au)

  4. Baseline Tc-99m DTPA renal scintigraphy as a predictor of outcome in children with urinary tract infection

    Energy Technology Data Exchange (ETDEWEB)

    Yun, J. G.; An, Y. S.; Lee, M. H.; Cho, C. W.; Yun, S. N.; Pai, G. S [Ajou University Medical Center, Suwon (Korea, Republic of)

    2004-07-01

    Tc-99m DTPA renal scintigraphy is useful in detecting urinary tract obstruction in patients with urinary tract infection (UTI). We evaluated the prognostic significance of baseline Tc-99m DTPA renal scintigraphy in children with UTI. Among children, who underwent both baseline/follow-up Tc-99m DMSA scintigraphies and baseline Tc-99m DTP A scintigraphy for evaluation of UTI, 32 patients with unilateral cortical defects on baseline Tc-99m DMSA scintigraphy were included in the study. The outcome of cortical defects was evaluated on follow-up Tc-99m DMSA scintigraphy by visual analysis. ROIs were drawn on the Tc-99m DPTA scintigraphy for calculation of ipsilateral to contralateral kidney ratio (ICR) at blood flow phase (< 60s, BFP) and cortical uptake phase (1-5 min, CUP). Median follow-up period of Tc-99m DMSA scintigraphy was 3.2 months (1.4 - 14 months). There were 24 patients with healing cortical defects and 8 with cortical scarring. Average ICRs of patients with healing defects were 1.11 {+-} 0.18 (0.44 - 1.57) at BFP and 0.97 {+-} 0.21 (0.31 - 1.28) at CUP, while those of patients with cortical scarring were 0.97 {+-} 0.47 (0.21 - 0.89) at BFP and 0.75 {+-} 0.49 (0.19 - 1.65) at CUP. ICR more than 0.9 was determined as a good prognostic indicator. The sensitivity, specificity, positive predictive value, and negative predictive value of ICR was 83.3%, 100%, 100% and 66.7% at BFP, and 79.0%, 62.5%, 86.4% and 50.0% at CUP. Ipsilateral to contralateral kidney ratio on baseline Tc-99m DPTA renal scintigraphy, especially at blood flow phase, is helpful in predicting outcome of children with UTI.

  5. Selective hepatic arterial infusion of In-111-DTPA-Phe{sup 1}-octreotide in neuroendocrine liver metastases

    Energy Technology Data Exchange (ETDEWEB)

    Limouris, Georgios S.; Kontogeorgakos, Dimitrios; Lyra, Maria; Dimitriou, Panagiotis; Stavraka, Anastasia [Athens Medical School, Divisions of Nuclear Medicine, Radiology I Department, Athens (Greece); Aretaieion University Hospital, Athens (Greece); Chatziioannou, Achilles; Mourikis, Dimitrios; Gouliamos, Athanassios; Vlahos, Lambros [Athens Medical School, Divisions of Angiography, Radiology I Department, Athens (Greece); Aretaieion University Hospital, Athens (Greece)

    2008-10-15

    The aim of this study is to evaluate the effectiveness of {sup 111}In-DTPA-Phe{sup 1}-octreotide infusions after selective catheterization of the hepatic artery in inoperable metastasised liver, sst{sub 2} receptor-positive neuroendocrine tumours due to the effect of {sup 111}In Auger electron emission, minimising in parallel the toxicity of non-target tissue. The average dose per session administered monthly to each patient (17 cases in total) was 6.3{+-}2.3 GBq. Repetitions did not exceed 12-fold, except in one case (15 sessions). Response assessment was classified according to the Response Evaluating Criteria in Solid Tumours. CT/MRI scans were performed as baseline before, during and after the end of treatment, and monthly ultrasound images for follow-up measurements. Toxicity (World Health Organization criteria) was measured using blood and urine tests of renal, hepatic and bone marrow function. Complete response was achieved in one (5.9%) patient and partial in eight (47.0%), and disease stabilization in 3(17.7%) patients; five (29.4%) did not respond. A 32-month median survival time was estimated in 12(70.5%). Nine of these 12 surviving had a mean target diameter shrinkage from 144 {+-} 81 to 60 {+-} 59 mm. Grade 1 erythro-, leuko- and thrombo-cytopenia occurred in three (17.6%) cases. In unresectable metastatic liver lesions positive for somatostatin receptors repeated, transhepatic high doses of {sup 111}In-DTPA-Phe{sup 1}-octreotide show an effective therapeutic outcome. Given the locoregional modality character of the administration technique plus the extremely short range of {sup 111}In Auger and internal conversion electrons emission, no nephro-, liver- or myelo-toxicity has so far been observed. (orig.)

  6. Detection of hepatocellular carcinoma in transgenic mice by Gd-DTPA- and rhodamine 123-conjugated human serum albumin nanoparticles in T1 magnetic resonance imaging.

    Science.gov (United States)

    Watcharin, Waralee; Schmithals, Christian; Pleli, Thomas; Köberle, Verena; Korkusuz, Hüdayi; Hübner, Frank; Waidmann, Oliver; Zeuzem, Stefan; Korf, Horst-Werner; Terfort, Andreas; Gelperina, Svetlana; Vogl, Thomas J; Kreuter, Jörg; Piiper, Albrecht

    2015-02-10

    Nanoparticle (NP)-based contrast agents that enable high resolution anatomic T1-weighted magnetic resonance imaging (MRI) offer the prospect of improving differential diagnosis of liver tumors such as hepatocellular carcinoma (HCC). In the present study, we investigated the possibility of employing novel non-toxic human serum albumin nanoparticles conjugated with Gd-DTPA and rhodamine 123 (Gd-Rho-HSA-NPs) for the detection of HCC by T1-weighted MRI. In addition, the influence of surface coating of the NPs with poloxamine 908, which alters the absorptive behavior of NPs and changes their distribution between the liver and tumor was examined. MRI of transgenic mice with endogenously formed HCCs following intravenous injection of Gd-Rho-HSA-NPs revealed a strong negative contrast of the tumors. Contrasting of the HCCs by NP-enhanced MRI required less Gd as compared to gadolinium-ethoxybenzyl-diethylenetriaminepentaacetic acid-enhanced MRI, which currently provides the most sensitive detection of HCC in patients. Immunohistochemical analyses revealed that the Gd-Rho-HSA-NPs were localized to macrophages, which were - similar to HCC in patients - fewer in number in HCC as compared to the liver tissue, which is in agreement with the negative contrasting of HCC in Gd-Rho-HSA-NP-enhanced MRI. Poloxamine-coated NPs showed lower accumulation in the tumor macrophages and caused a longer lasting enhancement of the MRI signal. These data indicate that Gd-Rho-HSA-NPs enable sensitive detection of HCC by T1-weighted MRI in mice with endogenous HCC through their uptake by macrophages. Poloxamine coating of the NPs delayed the tumor localization of the NPs. PMID:25499552

  7. Quantitative comparison of the regional distributions of inhaled Tc-99m DTPA aerosol and Kr-81m Gas in coal miners' lungs

    International Nuclear Information System (INIS)

    The authors compared the regional distribution of deposited Tc-99m DTPA aerosol (D) with that of Kr-81m (K) in the upright lungs of 22 coal miners with non-embolic pulmonary disease. Pixel-by-pixel distribution of K and D, as well as K/D ratios, were determined by computer analysis in each lung and presented as digitized posterior images. The lungs were also divided into 6.5-mm horizontal slices between apex and base. Values of the mean K and D counts and K/D ratio were plotted as functions of slice position. Good peripheral lung penetration was obtained for all subjects, while hot spots in the central airways were found in only one lung of a subject who smoked and had airway obstruction. Assessment of ventilation by visual evaluation of the respective images was comparable for both K and D. However, computer analysis showed that D was preferentially deposited in the basal regions of 38/44 lungs. K was preferentially distributed in the apical regions of 28/44 lungs. Increased bilateral D deposition in the basal regions was accompanied by a reduction in the apical regions of 10 subjects and in one lung of another 7 subjects. No relationship was found between aerosol deposition and ventilation/perfusion or pulmonary function measurements. In previous studies they have reported rapid aerosol clearance in coal miners. Although accurate clinical information has been obtained with D in patients with suspected pulmonary embolism, the effect of gravity on the deposition of D may limit its usefulness when quantitative information is required to evaluate subtle changes in ventilation in non-embolic pulmonary patients and for basic studies of ventilation and perfusion

  8. Food group diversity indicators derived from qualitative list-based questionnaire misreported some foods compared to same indicators derived from quantitative 24-hour recall in urban Burkina Faso.

    Science.gov (United States)

    Martin-Prevel, Yves; Becquey, Elodie; Arimond, Mary

    2010-11-01

    To be useful proxies of micronutrient intake at the population level in resource-limited societies, dietary diversity indicators should be simple and easy to collect in large surveys and their accuracy needs to be assessed. The present study aimed at comparing food group diversity indicators (FGI) derived from simple qualitative list-based questionnaires (qFGI) to the same indicators derived from quantitative 24-h recalls (QFGI). Both methods were administered separately on each of 3 recall days to women in 2 districts of Ouagadougou, Burkina Faso. Data were available for a total of 526 women x recalls. This study was performed within the framework of the Women's Dietary Diversity Project, which sought to analyze the relationships between various QFGI and the mean probability of adequacy (MPA) of women's diets across 11 micronutrients. The comparison between paired qFGI and QFGI scores was made both in terms of accuracy of the reporting by the list-based questionnaire, taking the QFGI as the gold standard, and in terms of performance of indicators in predicting an MPA > 60%. Examination of paired QFGI-qFGI differences revealed that the more disaggregated the FGI, the higher were the mean differences in scores. Food groups most frequently misreported often corresponded to foods put in small quantities in sauces. Overreporting by list-based questionnaires was observed for indicators applying a 15-g minimum quantity of consumption for a group to count in the score and this may result in weaker performance in predicting the MPA. These results highlight trade-offs between accuracy and simplicity when operationalizing FGI through qualitative questionnaires.

  9. Comparison of Cloud Base Height Derived from a Ground-Based Infrared Cloud Measurement and Two Ceilometers

    Directory of Open Access Journals (Sweden)

    Lei Liu

    2015-01-01

    Full Text Available The cloud base height (CBH derived from the whole-sky infrared cloud-measuring system (WSIRCMS and two ceilometers (Vaisala CL31 and CL51 from November 1, 2011, to June 12, 2012, at the Chinese Meteorological Administration (CMA Beijing Observatory Station are analysed. Significant differences can be found by comparing the measurements of different instruments. More exactly, the cloud occurrence retrieved from CL31 is 3.8% higher than that from CL51, while WSIRCMS data shows 3.6% higher than ceilometers. More than 75.5% of the two ceilometers’ differences are within ±200 m and about 89.5% within ±500 m, while only 30.7% of the differences between WSIRCMS and ceilometers are within ±500 m and about 55.2% within ±1000 m. These differences may be caused by the measurement principles and CBH retrieval algorithm. A combination of a laser ceilometer and an infrared cloud instrument is recommended to improve the capability for determining cloud occurrence and retrieving CBHs.

  10. Organic Light-Emitting Diodes Based on New Oxadiazole and Pyrazoline Derivatives

    Institute of Scientific and Technical Information of China (English)

    ZHANG Zhi-Ming; ZHANG Rui-Feng; WU Fang; MA Yu-Guang; LI Guo-Wen; TIAN Wen-Jing; SHEN Jia-Cong

    2000-01-01

    The bilayer heterojunction devices were fabricated successfully by using a novel oxadiazole derivative: 2, 2-(2,5-thiophenediyl) bis (5-(4-methyl) phenyl-1,3, 4-oxadiazole) (T-OXD) as the electron-transporting layer (ETL)and a pyrazoline derivative:1-phenyl-3-(dimethylamino)styryl-5-(p-(dimethylamino) phenyl)pyrazoline (PDP) as the light-emitting layer and the hole-transporting layer. The emission at 500nm was derived from PDP layer.In comparison with the bilayer device of tris (8-hydroxyquinoline) aluminum (Alq) as the ETL, the luminous efficiency of the PDP/T-OXD heterojunction device was enhanced by 104 times.

  11. Degradation of carbofuran derivatives in restricted water environments: basic hydrolysis in AOT-based microemulsions.

    Science.gov (United States)

    Morales, Jorge; Manso, José A; Cid, Antonio; Lodeiro, Carlos; Mejuto, Juan Carlos

    2012-04-15

    The effect of sodium bis(2-ethylhexyl)sulfosuccinate/isooctane/water microemulsions on the stability of 2,2-dimethyl-2,3-dihydro-1-benzofuran-7-yl methylcarbamate (carbofuran, CF), 3-hydroxy-2,3-dihydro-2,2-dimethylbenzofuran-7-yl methylcarbamate (3-hydroxycarbofuran, HCF) and 3-keto-2,3-dihydro-2,2-dimethylbenzofuran-7-yl methylcarbamate (3-ketocarbofuran, KCF) in basic media has been studied. The presence of these microheterogeneous media implies a large basic hydrolysis of CF and HCF on increasing surfactant concentration and, also, on increasing water content in the microemulsion. The hydrolysis rate constants are approximately 2- and 10-fold higher than those in pure water for HCF and CF, respectively. In contrast, a steep descent in the rate of decomposition for KCF was observed. These behaviours can be ascribed to the presence of CF derivatives both in the hydrophilic phase and in the lipophilic phase, while the hydroxyl ions are only restricted to the water pool of the microemulsion (hydrophilic phase). The kinetic rate constants for the basic hydrolysis in AOT-based microemulsions have been obtained on the basis of a pseudophase model. Taking into account that an important part of soils are colloids, the possibility of the presence of restricted water environments implies that soil composition and its structure will play an important role in the stability of these carbamates. In fact, we observed that the presence of these restricted aqueous media in the environment, in particular in watersheds and in wastewaters, could reduce significantly the half-life of these pesticides (33% and 91% for HCF and CF, respectively).

  12. On word medial destressing : an account based on the principle of economy of derivation

    OpenAIRE

    Ohta, Satoshi

    1993-01-01

    In the current stream of syntactic theory, economy princples which apply to both representations and derivations have attracted special attention (cf. Chomsky (1991, 1992)). The economy principles seem to have a kind of cognitively natural flavor and ...

  13. Intercellular imaging by a polyarginine derived cell penetrating peptide labeled magnetic resonance contrast agent,diethylenetriamine pentaacetic acid gadolinium

    Institute of Scientific and Technical Information of China (English)

    GUO You-min; LIU Min; YANG Jun-le; GUO Xiao-juan; WANG Si-cen; DUAN Xiao-yi; WANG Peng

    2007-01-01

    Background The cellular plasma membrane represents a natural barrier to many exogenous molecules including magnetic resonance (MR) contrast agent. Cell penetrating peptide (CPP) is used to internalize proteins, peptides, and radionuclide. This study was undertaken to assess the value of a new intracellular MR contrast medium, CPP labeled diethylenetriamine pentaacetic acid gadolinium (Gd-DTPA) in molecular imaging in vitro. Methods Fluorescein-5-isothiocyanate (FITC) and Gd-DTPA respectively labeled with CPP (FITC-CPP, Gd-DTPA-CPP) were synthesized by the solid-phase method. Human hepatic cancer cell line-HepG2 was respectively stained by FITC-CPP and FITC to observe the uptake and intracellular distribution. HepG2 was respectively incubated with 100 nmol/ml Gd-DTPA-CPP for 0, 10, 30, 60 minutes, and imaged by MR for studying the relationship between the incubation time and T1WI signal. The cytotoxicity to NIH3T3 fibroblasts cells was measured by 3-(4,5-dimethylthiazol-2-yl)- 2,5-diphenyltetrazolium bromide reduction assay (MTT). Results The molecular weights of CPP labeled imaging agents, which were determined by MALDI mass spectrometry (FITC-CPP MW=2163.34, Gd-DTPA-CPP MW=2285.99), were similar to the calculated molecular weights. Confocal microscopy suggested HepG2 translocated FITC-CPP in cytoplasm and nucleus independent with the incubation temperature. MR images showed HepG2 uptaken Gd-DTPA-CPP had a higher T1 weighted imaging (T1WI) signal, and that the T1WI signal intensity was increasing in a time-dependent manner (r=0.972, P=0.001), while the signal intensity between the cells incubated by Gd-DTPA for 60 minutes and the controlled cells was not significantly different (P=0.225). By MTT, all concentrations from 50 nmol/ml to 200 nmol/ml had no significant (F=0.006, P=1.000) effect on cell viability of mouse NIH3T3 fibroblasts, compared with the control group. Conclusions The newly constructed CPP based on polyarginine can translocate cells by carrying FITC

  14. Derivation of macroscopic equations for individual cell-based models. A formal approach

    OpenAIRE

    Bodnar, Marek; Velázquez, J. J. L.

    2005-01-01

    In this paper we review the theory of cells (particles) that evolve according to a dynamics determined by friction and that interact between themselves by means of suitable potentials. We derive by means of elementary arguments several macroscopic equations that describe the evolution of cell density. Some new results are also obtained — a formal derivation of a limit equation in the case of attractive potential as well as in the case of repulsive potential with a hard-core part are presented...

  15. Curriculum-Based Measurement of Oral Reading: Evaluation of Growth Estimates Derived with Pre-Post Assessment Methods

    Science.gov (United States)

    Christ, Theodore J.; Monaghen, Barbara D.; Zopluoglu, Cengiz; Van Norman, Ethan R.

    2013-01-01

    Curriculum-based measurement of oral reading (CBM-R) is used to index the level and rate of student growth across the academic year. The method is frequently used to set student goals and monitor student progress. This study examined the diagnostic accuracy and quality of growth estimates derived from pre-post measurement using CBM-R data. A…

  16. Spatial variability of soil total and DTPA-extractable cadmium caused by long-term application of phosphate fertilizers, crop rotation, and soil characteristics.

    Science.gov (United States)

    Jafarnejadi, A R; Sayyad, Gh; Homaee, M; Davamei, A H

    2013-05-01

    Increasing cadmium (Cd) accumulation in agricultural soils is undesirable due to its hazardous influences on human health. Thus, having more information on spatial variability of Cd and factors effective to increase its content on the cultivated soils is very important. Phosphate fertilizers are main contamination source of cadmium (Cd) in cultivated soils. Also, crop rotation is a critical management practice which can alter soil Cd content. This study was conducted to evaluate the effects of long-term consumption of the phosphate fertilizers, crop rotations, and soil characteristics on spatial variability of two soil Cd species (i.e., total and diethylene triamine pentaacetic acid (DTPA) extractable) in agricultural soils. The study was conducted in wheat farms of Khuzestan Province, Iran. Long-term (27-year period (1980 to 2006)) data including the rate and the type of phosphate fertilizers application, the respective area, and the rotation type of different regions were used. Afterwards, soil Cd content (total or DTPA extractable) and its spatial variability in study area (400,000 ha) were determined by sampling from soils of 255 fields. The results showed that the consumption rate of di-ammonium phosphate fertilizer have been varied enormously in the period study. The application rate of phosphorus fertilizers was very high in some subregions with have extensive agricultural activities (more than 95 kg/ha). The average and maximum contents of total Cd in the study region were obtained as 1.47 and 2.19 mg/kg and DTPA-extractable Cd as 0.084 and 0.35 mg/kg, respectively. The spatial variability of Cd indicated that total and DTPA-extractable Cd contents were over 0.8 and 0.1 mg/kg in 95 and 25 % of samples, respectively. The spherical model enjoys the best fitting and lowest error rate to appraise the Cd content. Comparing the phosphate fertilizer consumption rate with spatial variability of the soil cadmium (both total and DTPA extractable) revealed the high

  17. Cost comparison of {sup 111}In-DTPA-octreotide scintigraphy and {sup 68}Ga-DOTATOC PET/CT for staging enteropancreatic neuroendocrine tumours

    Energy Technology Data Exchange (ETDEWEB)

    Schreiter, Nils F.; Brenner, Winfried; Buchert, Ralph; Prasad, Vikas [Charite - Universitaetsmedizin Berlin, Department of Nuclear Medicine, Berlin (Germany); Nogami, Munenobu [University Hospital, Department of Radiology and Nuclear Medicine, Nankoku, Kochi (Japan); Huppertz, Alexander [Imaging Science Institute Charite Berlin, Berlin (Germany); Pape, Ulrich-Frank [Charite - Universitaetsmedizin Berlin, Department of Gastroenterology, Berlin (Germany); Hamm, Bernd; Maurer, Martin H. [Charite - Universitaetsmedizin Berlin, Department of Radiology, Berlin (Germany)

    2012-01-15

    Although somatostatin receptor positron emission tomography (PET)/CT is gaining increasing popularity and has shown its diagnostic superiority in several studies, {sup 111}In-diethylenetriaminepentaacetic acid (DTPA)-octreotide is still the current standard for diagnosis of neuroendocrine tumours (NET). The aim of this study was to compare the costs for the two diagnostic tests and the respective consequential costs. From January 2009 to July 2009, 51 consecutive patients with enteropancreatic NET who underwent contrast-enhanced {sup 68}Ga-DOTATOC PET/CT (n = 29) or {sup 111}In-DTPA-octreotide (mean 3 whole-body scans plus 1.6 low-dose single photon emission computed tomography/CT; n = 22) were included. For cost analysis, direct costs (equipment) and variable costs (material, labour) per examination were calculated. Additionally required CT and/or MRI examinations within the staging process were assessed as consequential costs. An additional deterministic sensitivity analysis was performed. A {sup 68}Ga-DOTATOC PET/CT examination yielded total costs (equipment, personnel and material costs) of 548 EUR. On the other hand, an {sup 111}In-DTPA-octreotide examination resulted in 827 EUR total costs. Costs for equipment and material had a share of 460 EUR/720 EUR for {sup 68}Ga-DOTATOC/{sup 111}In-DTPA-octreotide and labour costs of 89 EUR/106 EUR. With {sup 68}Ga-DOTATOC additional MRI had to be performed in 7% of the patients resulting in a mean of 20 EUR for supplementary imaging per patient; 82% of patients with {sup 111}In-DTPA-octreotide needed additional MRI and/or CT resulting in mean additional costs of 161 EUR per patient. {sup 68}Ga-DOTATOC PET/CT was considerably cheaper than {sup 111}In-DTPA-octreotide with respect to both material and personnel costs. Furthermore, by using {sup 68}Ga-DOTATOC PET/CT considerably fewer additional examinations were needed reducing the consequential costs significantly. (orig.)

  18. Synthesis, Characterization and Biological Activity of Transition Metals with Schiff Base Derived from Adamantaneamine and o-Vanillin

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    Five new solid complexes were synthesized about transition metals with Schiff base(L,C18H23NO2) derived from adamantaneamine and o-vanillin, and characterized by elemental analysis, molar conductance, infrared spectra, UV-vis spectra, thermal analysis. Their chemical formula are [ML2](ClO4)2 (M= Mn,Co,Ni,Cu,Zn),and the coordination numbers are four. The antibacterial activity of Schiff base ligand and its complexes was studied.

  19. Modeling Species Distribution Using Niche-Based Proxies Derived from Composite Bioclimatic Variables and MODIS NDVI

    OpenAIRE

    Hannes Feilhauer; He, Kate S.; Duccio Rocchini

    2012-01-01

    Vegetation mapping based on niche theory has proven useful in understanding the rules governing species assembly at various spatial scales. Remote-sensing derived distribution maps depicting occurrences of target species are frequently based on biophysical and biochemical properties of species. However, environmental conditions, such as climatic variables, also affect spectral signals simultaneously. Further, climatic variables are the major drivers of species distribution at macroscales. The...

  20. 信息动态%Preparation of Polysaccharide Derivatives-based Composite Chiral Stationary Phases and Their Chiral Recognition

    Institute of Scientific and Technical Information of China (English)

    2011-01-01

    Two coated-type composite chiral stationary phases (CSPs) were prepared based on cellulose tris(3,5-dimethylphenylcarbamate) (CDMPC) and amylose tris (3,5-dimethylphenylcarbamate)(ADMPC) by coating the corresponding derivatives onto 3-aminopropyl silica gel separately and then mixing or by coating the mixed derivatives onto silica gel. The CSPs containing only CDMPC or ADMPC were also prepared for comparison. The mixing method does not significantly influence the enantioselectivities. The composite CSPs generally show chiral recognition abilities intermediate between those of the two individual phases, while some racemates were poorer and at the same time one was better resolved on the composite CSPs.

  1. Lunar textural analysis based on WAC-derived kilometer-scale roughness and entropy maps

    Science.gov (United States)

    Li, Bo; Wang, XueQiang; Zhang, Jiang; Chen, Jian; Ling, Zongcheng

    2016-06-01

    In general, textures are thought to be some complicated repeated patterns formed by elements, or primitives which are sorted in certain rules. Lunar surfaces record the interactions between its outside environment and itself, thus, based on high-resolution DEM model or image data, there are some topographic features which have different roughness and entropy values or signatures on lunar surfaces. Textures of lunar surfaces can help us to concentrate on typical topographic and photometric variations and reveal the relationships between obvious features (craters, impact basins, sinuous rilles (SRs) and ridges) with resurfacing processes on the Moon. In this paper, the term surface roughness is an expression of the variability of a topographic or photometric surface at kilometer scale, and the term entropy can characterize the variability inherent in a geological and topographic unit and evaluate the uncertainty of predictions made by a given geological process. We use the statistical moments of gray-level histograms in different-sized neighborhoods (e.g., 3, 5, 10, 20, 40 and 80 pixels) to compute the kilometer-scale roughness and entropy values, using the mosaic image from 70°N to 70°S obtained by Lunar Reconnaissance Orbiter (LRO) Wide Angle Camera (WAC). Large roughness and entropy signatures were only found in the larger scale maps, while the smallest 3-pixel scale map had more disorderly and unsystematic textures. According to the entropy values in 10-pixel scale entropy map, we made a frequency curve and categorized lunar surfaces into three types, shadow effects, maria and highlands. A 2D scatter plot of entropy versus roughness values was produced and we found that there were two point clusters corresponding to the highlands and maria, respectively. In the last, we compared the topographic and photometric signatures derived from Lunar Orbiter Laser Altimeter (LOLA) data and WAC mosaic image. On the lunar surfaces, the ridges have obvious multilevel

  2. Diester Derivatives from Chemically Modiifed Waste Cooking Oil as Substitute for Petroleum Based Lubricating Oils

    Institute of Scientific and Technical Information of China (English)

    Xiang Shuo; Chen Ligong; Xu Lan; Li Liang; Yang Xin; Zhu Liye

    2015-01-01

    In order to provide a new way for waste cooking oil (WCO) resource utilization, several diester derivatives were obtained from WCO through a three-step chemical modifications, viz.:transesterification, epoxidation and oxirane ring opening with carboxylic acids. The effects of the chain length of side chain groups on the viscosity, acid value, low temper-ature lfuidity, thermo-oxidative stability, tribological properties and surface tension of diester derivatives were investigated. The results showed that increasing the chain length of side chain groups had a positive inlfuence on the viscosity, viscosity index, acid value, pour point, friction coefifcient and wear scar diameter along with a negative inlfuence on the oxidation onset temperature, volatile loss, insoluble deposit, maximum non-seizure load and surface tension. These diester derivatives exhibited improved physicochemical and tribological properties that make themselves promising environmentally friendly biolubricant basestocks.

  3. Synthesis and Antiproliferative Activities of Benzimidazole-Based Sulfide and Sulfoxide Derivatives

    OpenAIRE

    Gaballah, Samir T.; El-Nezhawy, Ahmed O. H.; Amer, Hassan; Ali, Mamdouh Moawad; Mahmoud, Abeer Essam El-Din; Hofinger-Horvath, Andreas

    2015-01-01

    The design, synthesis, and in vitro antiproliferative activity of a novel series of sulfide (4a–i) and sulfoxide (5a–h) derivatives of benzimidazole, in which different aromatic and heteroaromatic acetamides are linked to benzimidazole via sulfide (4a–i) and sulfoxide (5a–h) linker, are reported and the structure-activity relationship is discussed. The new derivatives were prepared by coupling 2-(mercaptomethyl)benzimidazole with 2-bromo-N-(substituted) acetamides in dry acetone in the presen...

  4. Novel self-associative and multiphase nanostructured soft carriers based on amphiphilic hyaluronic acid derivatives

    DEFF Research Database (Denmark)

    Eenschooten, Corinne; Vaccaro, Andrea; Delie, Florence;

    2012-01-01

    The purpose of the present study was to investigate the physicochemical properties in aqueous media of amphiphilic hyaluronic acid (HA) derivatives obtained by reaction of HA’s hydroxyl groups with octenyl succinic anhydride (OSA). The self-associative properties of the resulting octenyl succinic...... anhydridemodified hyaluronic acid (OSA-HA) derivatives were studied by fluorescence spectroscopy using Nile Red as fluorophore. The morphology, size and surface charge of the OSA-HA assemblies were determined by transmission electron microscopy, dynamic light scattering and by measuring their electrophoretic...

  5. Effects of Gd-DTPA on Prostate 1H-MR Spectroscopy%Gd-DTPA对前列腺3T氢质子波谱影响的初步研究

    Institute of Scientific and Technical Information of China (English)

    杨春; 徐凯; 郑骏年; 许倩; 马红; 荣玉涛

    2011-01-01

    Purpose: Using high field MR system to study whether the result of 1H - MR spectroscopy (MRS) could be influenced by Gd- DTPA.Methods: 1H - MRS examinations were taken before and after administration of Gd- DTPA on 30 cases.Line width (LW), water suppression (WS%) and spectroscopy quality before and after administration of Gd- DTPA were compared.Results: LW of spectroscopy after administration of Gd - DTPA (14.41 ± 1.78) was increased than before (12.25 ± 1.36) (P < 0.05).WS%before and after administration of Gd- DTPA showed no statistical difference(P > 0.05).Spectroscopy quality of 1H - MRS after administration of Gd- DTPA was decreased than before(P < 0.05).Conclusion: Gd- DTPA can change the LW and spectroscopy quality of prostate 1H - MRS at 3 T.%目的:用高场MR仪研究对比剂(Gd-DTPA)是否会影响前列腺氧质子MR波谱(H-MRS)的线宽(LW)、抑水率(WS%)及谱线质量.方法:对连续30例行前列腺MR波谱检查的患者分别于增强扫描前、后行H-MRS检查,比较扫描前、后两次LW、WS%及谱线质量.结果:增强后LW(14.41±1.78)较增强前(12.25±1.36)增高(P0.05).增强后的谱线质量降低(PH-MRS的LW及谱线质量.

  6. Synthesis and NMR study of N-heterocyclic carbenes (NHC precursors derived from Tröger’s Base

    Directory of Open Access Journals (Sweden)

    Eric MUSENGIMANA

    2014-01-01

    Full Text Available Since the discovery of olefin metathesis reactions, various catalysts have been developed but those based on Ruthenium and N-heterocyclic carbenes (NHC ligands are particularly more efficient. Herein, we report a synthesis of NHC precursors derived from the Tröger’s base and its analogues which can be used in the synthesis of the new kind of metathesis catalysts. The NHC derived from the Tröger’s base present a particular chirality that could affect stereoselectivity of catalysis, and the form “V” of these ligands implies that the aromatic substituents point totally outside the coordination sphere of the metal, where would be no steric hindrance unlike what happens with the usual NHC.

  7. Synthesis and Chromatographic Properties of New β-Cyclodextrin Derivatives with α-Schiff Base Groups for HPLC

    Institute of Scientific and Technical Information of China (English)

    Min FANG; Zhi Ming ZHOU; Ai Qin LUO

    2005-01-01

    β-Cyclodextrin 1 was directly oxidized to the corresponding monoaldehyde 2 on their primary faces by cyclized 2-iodoxybenzoic acid(IBX) in DMSO, followed by the synthesis of β-cyclodextrin derivatives bearing Schiff-base group 3. A new chiral stationary phase(BCDS 6)was then prepared by immobilization of β-cyclodextrin derivative with α-Schiff base group onto the surface of sillica gel. A series of compounds with amino groups were readily separated using this CSP. Methanol and acetonitrile were tested as the mobile phase while the influence of temperature and the addition of aqueous triethylammonium acetate buffer to the mobile phase was also innvestigated. Ferrocene ligand with Schiff-based groups have been separated satisfactorilly on BCDS column.

  8. Numerical simulation of aerodynamic derivatives and critical wind speed for long-span bridges based on simplified steady wind field

    Institute of Scientific and Technical Information of China (English)

    Xin Dabo; Ou Jinping

    2007-01-01

    Combining the computational fluid dynamics-based numerical simulation with the forced vibration technique for extraction of aerodynamic derivatives, an approach for calculating the aerodynamic derivatives and the critical flutter wind speed for long-span bridges is presented in this paper. The RNG κ-ε turbulent model is introduced to establish the governing equations, including the continuity equation and the Navier-Stokes equations, for solving the wind flow field around a two-dimensional bridge section. To illustrate the effectiveness and accuracy of the proposed approach, a simple application to the Hume Bridge in China is provided, and the numerical results show that the aerodynamic derivatives and the critical flutter wind speed obtained agree well with the wind tunnel test results.

  9. Wave simulation in biologic media based on the Kelvin-Voigt fractional-derivative stress-strain relation.

    Science.gov (United States)

    Caputo, Michele; Carcione, José M; Cavallini, Fabio

    2011-06-01

    The acoustic behavior of biologic media can be described more realistically using a stress-strain relation based on fractional time derivatives of the strain, since the fractional exponent is an additional fitting parameter. We consider a generalization of the Kelvin-Voigt rheology to the case of rational orders of differentiation, the so-called Kelvin-Voigt fractional-derivative (KVFD) constitutive equation, and introduce a novel modeling method to solve the wave equation by means of the Grünwald-Letnikov approximation and the staggered Fourier pseudospectral method to compute the spatial derivatives. The algorithm can handle complex geometries and general material-property variability. We verify the results by comparison with the analytical solution obtained for wave propagation in homogeneous media. Moreover, we illustrate the use of the algorithm by simulation of wave propagation in normal and cancerous breast tissue. PMID:21601139

  10. Investigation of the retention and distribution of americium-241 in the baboon and the enhanced removal of americium-241 from the body by diethylenetriaminepentaacetic acid (DTPA)

    International Nuclear Information System (INIS)

    Experiments were performed to study the metabolism and distribution of intravenously administered 241Am in the adult and juvenile baboon; in addition, decorporation therapy using Na3-CaDTPA was performed on selected baboons to assess the efficacy of this drug in removing systemic burdens of 241Am from this primate species. Determination of the kinetics of 241Am was accomplished principally by in vivo methodologies and by radiochemical analysis of 241Am activity of biological material. The use of Na3-CaDTPA as a therapeutic agent for the removal of 241Am from the body proved to be an effective form of treatment in the case of early administration. (U.S.)

  11. Comparison of two brain tumor-localizing MRI agent. GD-BOPTA and GD-DTPA. MRI and ICP study of rat brain tumor model

    International Nuclear Information System (INIS)

    In this study, we compared the behavior of Gd-BOPTA as a brain tumor selective contrast agent with Gd-DTPA in a common dose of 0.1 mmol/kg. We performed a MRI study using those two agent as contrast material, and we measured tissue Gd-concentrations by ICP-AES. As a result, Gd-BOPTA showed a better MRI enhancement in brain tumor. ICP showed significantly greater uptake of Gd-BOPTA in tumor samples, at all time course peaked at 5 minutes after administration, Gd being retained for a longer time in brain tumor till 2 hours, without rapid elimination as Gd-DTPA. We conclude that Gd-BOPTA is a new useful contrast material for MR imaging in brain tumor and an effective absorption agent for neutron capture therapy for further research. (author)

  12. A derivative based simplified phase tracker for a single fringe pattern demodulation

    Science.gov (United States)

    Deepan, B.; Quan, C.; Tay, C. J.

    2016-08-01

    In this paper, a novel fringe demodulation method for the estimation of phase and its first-order derivative from a closed-fringe interferogram is proposed. The proposed method determines the phase derivatives in both x&y directions from fringe orientation and density. The phase derivatives are subsequently used to determine phase values using a novel simplified phase tracker. In the phase tracking model, the complexity of the cost function is reduced using predetermined derivatives so computation time required for phase tracking is reduced considerably. The proposed model is more robust while dealing with saddle points in fringes than the conventional phase tracker model. Hence it does not require any specialized scanning strategy. The proposed method is validated with simulated and experimental fringe patterns (obtained using electronic speckle pattern interferometry and optical holographic interferometry) and a comparison study is carried out with conventional regularized phase tracker. The simulation results show that the proposed method has good accuracy and requires less computation time than existing phase-tracking algorithms. The experimental results demonstrate the robustness of the proposed method against speckle noise and its practical applicability for static and dynamic applications.

  13. Bioassay based screening of steroid derivatives in animal feed and supplements

    NARCIS (Netherlands)

    Rijk, J.C.W.; Ashwin, H.M.; Kuijk, van S.J.A.; Groot, M.J.; Heskamp, H.H.; Bovee, T.F.H.; Nielen, M.W.F.

    2011-01-01

    Receptor binding transcription activation bioassays are valuable tools for the screening of steroid hormones in animal feed and supplements. However, steroid derivatives often lack affinity for their cognate receptor and do not show any direct hormonal activity by themselves. These compounds are thu

  14. Assembly of twisted luminescent architectures based on acenaphtho[1,2-k]fluoranthene derivatives.

    Science.gov (United States)

    Han, Liang; Zhang, Yuewei; Chen, Weiping; Cheng, Xiao; Ye, Kaiqing; Zhang, Jingying; Wang, Yue

    2015-03-14

    Acenaphtho[1,2-k]fluoranthene derivatives DPAF-n as new building blocks for one-dimensional (1D) structure assembly have been developed and employed to fabricate luminescent twisted nano/micro-wires; and the DPAF rigid core attached via flexible alkyl chains with suitable lengths is critical for the formation of twisted architectures. PMID:25682899

  15. Development of a Cell-based Model to Derive Direct Runoff Hydrographs for Ungauged Mountainous Basins

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    A model to derive direct runoff hydrograph for an ungauged basin using the physical properties of the basin is presented. The basin is divided into grid cells and canal elements. Overland flow is generated from each grid cell of the basin by application of continuous effective rainfall of 1 mm/hr to the basin. The flow generated is routed through downstream grid cells and the canal elements using the kinematic wave approach. The travel time for direct runoff from each grid cell to the basin outlet is calculated and the S-curve is derived for the basin.The S-curve is used to derive the unit hydrograph of a given duration for the basin. The model, referred as Cell-basin model was applied to the Upper Kotmale Basin in Sri Lanka and the model predictions of direct runoff hydrographs for rainfall events agreed with the observations to a reasonable accuracy. Comparison of the unit hydrographs obtained from the model and from the conventional Snyder's synthetic unit hydrograph using regionalized parameters assuming the basin as an ungauged basin, with the unit hydrograph derived from the observations showed that the model predicted unit hydrograph was more suitable than that obtained by Snyder's method for Sri Lankan up country basins. Thus, the present model is a useful tool to obtain direct runoff hydrograph for ungauged basins.

  16. Highly efficient non-doped blue organic light emitting devices based on anthracene–pyridine derivatives

    Energy Technology Data Exchange (ETDEWEB)

    Haykir, Gulcin; Tekin, Emine; Atalar, Taner; Türksoy, Figen

    2013-12-02

    Four different 2-(10-aryl)anthracen-9-yl)pyridine derivatives 5a–d were synthesized via the Suzuki cross-coupling reaction. Photo-physical characteristics of these materials having strong electron donating or electron withdrawing groups were explored. Multilayer small molecule organic light emitting diodes without any dopant were fabricated in the following sequence: Indium tin oxide/4,4′-bis(N-(1-naphthyl)-N-phenylamino)biphenyl (50 nm)/5a–d (30 nm)/4,7-diphenyl-1,10-phenanthroline (30 nm)/LiF/Al. The electroluminescent property of the device fabricated with 5d as an emitter exhibited a high external quantum efficiency of 3.80% (at around 1 mA/cm{sup 2}) with Commission Internationale De L'Eclairage coordinates of (0.14, 0.25). - Highlights: • Synthesis and characterization of 2-(10-aryl)anthracen-9-yl)pyridine derivatives • Thermal, photophysical and electrochemical properties of anthracene derivatives • Emitters from blue to greenish blue for organic light emitting device applications • Organic light emitting device fabrication and characterization of 2-(10-aryl)anthracen-9-yl)pyridine derivatives.

  17. Boundary Value Technique for Initial Value Problems Based on Adams-Type Second Derivative Methods

    Science.gov (United States)

    Jator, S. N.; Sahi, R. K.

    2010-01-01

    In this article, we propose a family of second derivative Adams-type methods (SDAMs) of order up to 2k + 2 ("k" is the step number) for initial value problems. The methods are constructed through a continuous approximation of the SDAM which is obtained by multistep collocation. The continuous approximation is used to obtain initial value methods,…

  18. Real-Time Algebraic Derivative Estimations Using a Novel Low-Cost Architecture Based on Reconfigurable Logic

    Directory of Open Access Journals (Sweden)

    Rafael Morales

    2014-05-01

    Full Text Available Time derivative estimation of signals plays a very important role in several fields, such as signal processing and control engineering, just to name a few of them. For that purpose, a non-asymptotic algebraic procedure for the approximate estimation of the system states is used in this work. The method is based on results from differential algebra and furnishes some general formulae for the time derivatives of a measurable signal in which two algebraic derivative estimators run simultaneously, but in an overlapping fashion. The algebraic derivative algorithm presented in this paper is computed online and in real-time, offering high robustness properties with regard to corrupting noises, versatility and ease of implementation. Besides, in this work, we introduce a novel architecture to accelerate this algebraic derivative estimator using reconfigurable logic. The core of the algorithm is implemented in an FPGA, improving the speed of the system and achieving real-time performance. Finally, this work proposes a low-cost platform for the integration of hardware in the loop in MATLAB.

  19. Derivation of site-specific relationships between hydraulic parameters and p-wave velocities based on hydraulic and seismic tomography

    Energy Technology Data Exchange (ETDEWEB)

    Brauchler, R.; Doetsch, J.; Dietrich, P.; Sauter, M.

    2012-01-10

    In this study, hydraulic and seismic tomographic measurements were used to derive a site-specific relationship between the geophysical parameter p-wave velocity and the hydraulic parameters, diffusivity and specific storage. Our field study includes diffusivity tomograms derived from hydraulic travel time tomography, specific storage tomograms, derived from hydraulic attenuation tomography, and p-wave velocity tomograms, derived from seismic tomography. The tomographic inversion was performed in all three cases with the SIRT (Simultaneous Iterative Reconstruction Technique) algorithm, using a ray tracing technique with curved trajectories. The experimental set-up was designed such that the p-wave velocity tomogram overlaps the hydraulic tomograms by half. The experiments were performed at a wellcharacterized sand and gravel aquifer, located in the Leine River valley near Göttingen, Germany. Access to the shallow subsurface was provided by direct-push technology. The high spatial resolution of hydraulic and seismic tomography was exploited to derive representative site-specific relationships between the hydraulic and geophysical parameters, based on the area where geophysical and hydraulic tests were performed. The transformation of the p-wave velocities into hydraulic properties was undertaken using a k-means cluster analysis. Results demonstrate that the combination of hydraulic and geophysical tomographic data is a promising approach to improve hydrogeophysical site characterization.

  20. MR pulmonary ventilation information of canines inhaling aerosolized gadolinium-DTPA%应用雾化Gd DTPA获得犬肺通气信息

    Institute of Scientific and Technical Information of China (English)

    郭佑民; 吴晓明; 杨健; 王建国; 雷晓燕

    2003-01-01

    AIM:To investigate the effect of inhaling aerosolized gadolinium DTPA (Gd-DTPA) on pulmonary MR imaging in canines. METHODS:Six healthy northern dogs were performed MR scanning by using single shot turbo spin echo sequence.The changes of signal intensity (SI) in pulmonary parenchyma were respectively measured after inhaling air gas,aerosolized sodium chloride and Gd-DTPA as contrast agents.The differences of SI were compared between them. RESULTS:The SI of pulmonary parenchyma increased by 11.5%- 25.8% after inhalation of aerosolized sodium chloride solution, with no statistic difference compared to inhalation of air(t=2.798,P >0.05). Whereas the SI after inhalation of aerosolized Gd DTPA increased much higher by 42.7%- 76.8% than that after inhalation of air and aerosolized sodium chloride solution(t=4.660, P0.05);吸入 Gd-DTPA雾化颗粒后肺实质信号强度明显增加,增强幅度 42.7~ 76.8%,平均 59.2%,与吸入空气时的信号强度相比有统计学差异 (t=4.660,P< 0.05). 结论 :雾化的 Gd-DTPA可以作为一种有效的肺部 MRI增强对比剂,应用雾化 Gd DTPA来获得大型实验动物的有关肺通气信息是可行的.

  1. Long-term effects of inhaled Ca-DTPA in rats previously exposed to 239Pu(NO3)4 aerosols

    International Nuclear Information System (INIS)

    Inhaled Ca-DTPA given 20 days after 239Pu(NO3)4 inhalation was marginally effective (p = 0.10) in reducing the amount of Pu in rat lung. This reduction in Pu lung burden did not, however, affect the later induction of lung tumors. An increased osteosarcoma incidence was noted in control rats exposed only to nitric acid aerosols. Complete tumor incidence data are not yet available. (U.S.)

  2. MRI enhancement of the facial nerve with Gd-DTPA, 1; Experimental study on the enhancement mechanism used in viewing vascular permeability of the facial nerve

    Energy Technology Data Exchange (ETDEWEB)

    Yanagida, Masahiro (Kansai Medical School, Moriguchi, Osaka (Japan))

    1993-08-01

    Although there have recently been numerous reports of enhanced MRI in patients with facial palsy, the mechanism of enhancement remains largely unknown. In the present study, animal models with experimentally induced facial paralysis were prepared, and the vascular permeabilities of normal and damaged facial nerves were assessed using Evans blue albumin (EBA) as a tracer. The Gd-DTPA contents in normal and compressively damaged facial nerves were also investigated. In the normal intratemporal facial nerve, EBA remained in the vessels, and did not leak into the endoneurium. In contrast, vascular permeability was very high in the epineurium and the geniculate ganglion which showed leakage of large amounts of EBA from vessels. At the site of compression in the damaged nerve, EBA leakage was also seen in the endoneurism, indicating accentuated vascular permeability. This accentuation of vascular permeability shifted toward the distal side. However, no EBA leakage was seen on the side proximal to the site of compression. Significantly higher Gd-DTPA contents were obtained in the facial nerve on the paralytic side than in that on the normal side (p<0.001). As for differences between the distal and proximal sides, the distal side had a significantly higher Gd-DTPA content (p<0.01). Assessment of vascular permeability with EBA revealed accentuated vascular permeability on the side distal to the site of compression. These results showed the presence of a blood nerve barrier (BNB) in the facial nerve. Furthermore, the present findings suggest that the enhancement of the facial nerve on the affected side is caused by BNB destruction due to nerve damage and subsequent Gd-DTPA leakage from the vessels. Furthermore, it is suggested that the facial nerve enhancement appears to occur mainly on the distal side of the damaged portion of the nerve. (author).

  3. [Quantification of separate renal function using Tc 99m DTPA and Tc 99m DMSA. Correlations between individual isotopic data and creatinine clearance].

    Science.gov (United States)

    Chevet, D; Moisan, A; Le Pogamp, P; Le Cloirec, J; Wehbe, B; Herry, J Y

    1984-01-01

    The purpose of this prospective study (30 controls and 100 renal patients) is to compare one to each other the values of renal function measured with: 1--the DTPA 99mTc , 2--the DMSA 99mTc , and 3--the creatinine clearance. After the control values being established, correlations are done for the whole group (n = 130) between the creatinine clearance, the DTPA 99mTc clearance and the fixation rate of the DMSA 99mTc . The correlations obtained are:--Clcr Versus Cldtpa = 0,91.--Clcr Versus % Dmsa = 0,90. - Cldtpa Versus % Dmsa = 0,93. and indicate the two isotopic tests performed give similar quantitative results than creatinine clearance. Camera and computer allow to measure the separate renal function one by one, the two isotopic test giving similar values for each kidney. The results of these studies give a good concordance with the observed diseases, except for partial renal artery stenosis. The pyelic retention of DMSA 99mTc product, in condition of ureteral compression, gives a potential overestimation of this test and is a matter to debate. The extreme simplicity and facility of DMSA 99mTc procedures make this investigation accessible to any patient and give excellent morphological and functional tests. DTPA 99mTc clearances--global and separate kidney--give a more sensitive approach than DMSA 99mTc does because vascular captation , elimination rates could be focused. DTPA 99mTc procedure is to be used in patients with transplanted kidney, silent IVP kidney, contrast media contraindication or for exploring glomerular clearance without urine collection.

  4. QC studies and biological evaluations of three locally produced renal pharmaceutical preparations: 99mTc-DTPA, 99mTc-GH, and 99mTc- DMSA

    International Nuclear Information System (INIS)

    Quality control (QC) studies were done of three locally produced renal preparations of 99mTc- DTPA, 99mTc-GH and 99mTc-DMSA, as part of the production activity before the Gulf war. These studies were performed using gel chromatography column scanning (GCS) technique, although there are other alternative techniques. These kits were tested for radiochemical purity, and the labeling yields were found to be excellent. Biological studies included the following parameters: organ distribution (in mice, rats and rabbits), blood clearance in rabbits, and plasma protein binding in rats at different time intervals. Poor renal concentration of three agents in mice was significant at two time intervals. The tissue distribution in mice of DTPA, GH, and DMSA kits, which were stored at 37 deg. C for 30 days, after intravenous injection of the radioactivity at 5, 30, and 60 minutes, respectively, is presented. Blood retention of the renal agents was considerably low compared to those in rats and rabbits at the respective time intervals. The blood clearance of 99mTc-DMSA was relatively slow, and 99mTc-GH has rapid blood clearance, identical with that of 99mTc-DTPA for the first 20 minutes but slower thereafter. The binding of DTPA, GH and DMSA with plasma protein was 5, 65, 95 % at 1 h, respectively. Various amounts of tin (II) (as SnCl2), were used in the kits, with up to 10 times more of the usual dose for respective tin-complex. It was observed that there was no significant variation of the organ distribution in mice

  5. Schiff Base Metal Derivatives Enhance the Expression of HSP70 and Suppress BAX Proteins in Prevention of Acute Gastric Lesion

    Directory of Open Access Journals (Sweden)

    Shahram Golbabapour

    2013-01-01

    Full Text Available Schiff base complexes have appeared to be promising in the treatment of different diseases and disorders and have drawn a lot of attention to their biological activities. This study was conducted to evaluate the regulatory effect of Schiff base metal derivatives on the expression of heat shock proteins (HSP 70 and BAX in protection against acute haemorrhagic gastric ulcer in rats. Rats were assigned to 6 groups of 6 rats: the normal control (Tween 20 5% v/v, 5 mL/kg, the positive control (Tween 20 5% v/v, 5 mL/kg, and four Schiff base derivative groups named Schiff_1, Schiff_2, Schiff_3, and Schiff_4 (25 mg/kg. After 1 h, all of the groups received ethanol 95% (5 mL/kg but the normal control received Tween 20 (Tween 20 5% v/v, 5 mL/kg. The animals were euthanized after 60 min and the stomachs were dissected for histology (H&E, immunohistochemistry, and western blot analysis against HSP70 and BAX proteins. The results showed that the Schiff base metal derivatives enhanced the expression of HSP70 and suppressed the expression of BAX proteins during their gastroprotection against ethanol-induced gastric lesion in rats.

  6. Marginal erosive discovertebral ''Romanus'' lesions in ankylosing spondylitis demonstrated by contrast enhanced Gd-DTPA magnetic resonance imaging

    Energy Technology Data Exchange (ETDEWEB)

    Jevtic, V. [Clinical Radiology Institute, University Clinical Centre, Ljubljana (Slovenia); Kos-Golja, M.; Rozman, B. [Department of Rheumatology, University Clinical Centre, Ljubljana (Slovenia); McCall, I. [Robert Jones and Agnes Hunt Orthopaedic and District Hospital, Oswestry, Shropshire (United Kingdom)

    2000-01-01

    Objective. To assess the value of Gd-DTPA magnetic resonance (MR) imaging in the demonstration of marginal destructive discovertebral Romanus lesions in ankylosing spondylitis.Design and patients. A prospective study of Gd-DTPA MR imaging was performed in 39 patients with a clinical diagnosis of ankylosing spondylitis and typical Romanus lesions seen on radiographs of the thoracolumbar spine. MR morphological appearances and signal intensity changes at the discovertebral junctions were analysed and compared with the radiographic findings.Results. Ninety-nine discovertebral junctions with Romanus lesions showed low signal intensity on T1-weighted and high signal on T2-weighted and T1-weighted postcontrast images at the vertebral corners consistent with oedematous hyperaemic inflammatory tissue. There were nine discovertebral junctions with similar MR findings but normal radiographs. Fifty-three discovertebral junctions showed syndesmophyte formation with increased signal intensity on both T1- and T2-weighted images with no contrast enhancement. Sixty-five discovertebral junctions showed a mixture of radiographic features and varied high and low signal changes at the vertebral rim on MR imaging with rims of enhancement in the vertebral body following contrast administration.Conclusion. Gd-DTPA MR imaging demonstrates a variable signal pattern and degree of contrast enhancement which may reflect the evolutionary stages of discovertebral enthesitis in ankylosing spondylitis. MR imaging may identify early erosive changes in radiographically normal vertebra. The role of MR imaging needs further investigation. (orig.)

  7. Treatment with high dose [111In-DTPA-D-PHE1]-octreotide in patients with neuroendocrine tumors. Evaluation of therapeutic and toxic effects

    International Nuclear Information System (INIS)

    Carcinoid tumors and endocrine pancreatic tumors often express somatostatin receptors (sst). Tumor spread may be visualized by sst scintigraphy using [111In-DTPA-D-Phe1]-octreotide. In this study, tumor targeting therapy with [111In-DTPA-D-Phe1]-octreotide at high doses (6 GBq every third week) was used to treat patients with sst-expressing tumors. Five patients entered the protocol and three were evaluable for response, while all could be evaluated for toxicity. Two patient responded with a significant reduction in tumor markers (> 50%). The third patient showed increasing levels of tumor markers. Side effects were expressed as depression of bone-marrow function. In one patient a grade 4 reduction in platelet count was observed requiring several thrombocyte transfusions. In another two patients platelet counts decreased significantly. We conclude that treatment with [111In-DTPA-D-Phe1]-octreotide can be used in patients with neuroendocrine tumors but blood parameters have to be carefully monitored to avoid severe side effects. (orig.)

  8. {sup 177}Lu-labeled-VG76e monoclonal antibody in tumor angiogenesis: a comparative study using DOTA and DTPA chelating systems

    Energy Technology Data Exchange (ETDEWEB)

    Fani, M.; Psimadas, D. [Inst. of Radioisotopes and Radiodiagnostic Products, National Centre for Scientific Research ' ' Demokritos' ' , Athens (Greece); Biomedica Life Sciences S.A., Athens (Greece); Bouziotis, P.; Gourni, E.; Varvarigou, A.D. [Inst. of Radioisotopes and Radiodiagnostic Products, National Centre for Scientific Research ' ' Demokritos' ' , Athens (Greece); Harris, A.L. [Weatherall Inst. of Molecular Medicine, Cancer Research U.K., Univ. of Oxford (United Kingdom); Loudos, G. [Biomedical Simulations and Imaging Lab., National Technical Univ. of Athens (Greece); Maecke, H.R. [Div. of Radiological Chemistry, Univ. Hospital Basel (Switzerland)

    2007-07-01

    Vascular endothelial growth factor (VEGF) is one of the molecules which regulate angiogenesis, a phenomenon observed in many diseases, including cancer. VG76e, an anti-VEGF monoclonal antibody, was labeled with {sup 177}Lu via p-SCN-Bz-DOTA and CHX-A''-DTPA chelating systems, in order to investigate its possible therapeutic use. Labeling was performed by a 30 min incubation of {sup 177}LuCl{sub 3} and each immunoconjugate, at 37 C. Radiochemical analysis showed the formation of a single radioactive species, at a yield higher than 98%, for both immunoconjugates. Kits have been formulated for both VG76e-DOTA and VG76e-DTPA. Stability studies, in the presence of a competitor excess, showed that both radiolabeled species remained sufficiently stable (95%) for at least 48 h. Biodistribution results in normal mice were similar for both radioimmunoconjugates, with no significant bone uptake. Gamma camera images of tumor-bearing mice showed satisfactory visualization of the tumor 24 h p.i., while a higher uptake was observed at 48 h p.i. Our findings indicate that both the bifunctional chelating agents p-SCN-Bz-DOTA and CHX-A''-DTPA can be used for the labeling of VG76e with {sup 177}Lu, with high labeling yield and stability. Their in vivo behaviour in normal and tumor-bearing mice looks promising and they can be successfully used for tumor imaging studies. (orig.)

  9. Contribution of the hepatobiliary phase of Gd-EOB-DTPA-enhanced MRI to Dynamic MRI in the detection of hypovascular small ({<=}2 cm) HCC in cirrhosis

    Energy Technology Data Exchange (ETDEWEB)

    Golfieri, Rita; Renzulli, Matteo; Lucidi, Vincenzo; Corcioni, Beniamino [University of Bologna, Radiology Unit, Department of Digestive Diseases and Internal Medicine, Sant' Orsola-Malpighi Hospital, Bologna (Italy); Trevisani, Franco [University of Bologna, Unit of Semeiotica, Department of Digestive Diseases and Internal Medicine, Sant' Orsola-Malpighi Hospital, Bologna (Italy); Bolondi, Luigi [University of Bologna, Unit of Internal Medicine, Department of Digestive Diseases and Internal Medicine, Sant' Orsola-Malpighi Hospital, Bologna (Italy)

    2011-06-15

    To prospectively assess the additional value of the hepatobiliary (HB) phase of Gd-EOB-DTPA-MRI in identifying and characterising small ({<=}2 cm) hepatocellular carcinomas (HCCs) undetermined in dynamic phases alone because of their atypical features, according to the AASLD criteria. 127 cirrhotic patients were evaluated with Gd-EOB-DTPA-MRI in two sets: unenhanced and dynamic phases; unenhanced, dynamic and HB phases. Sixty-two out of 215 nodules (29%) were atypical in 42 patients (33%). 62 atypical nodules were reported at histology: high-grade dysplastic nodules (HGDN)/early HCC (n = 20), low-grade DN (LGDN) (n = 21), regenerative nodules (n = 17) and nodular regenerative hyperplasia (n = 4). The sensitivity, specificity, accuracy, positive and negative predictive value (PPV, NPV) were increased by the addition of the HB phase: 88.4-99.4%, 88-95%, 88-98.5%, 97-99%, and 65-97.5%, respectively. Twenty atypical nodules were malignant (32%), 19 of which were characterised only during the HB phase. The HB phase is 11% more sensitive in the classification of HGDN/early HCC than dynamic MRI, with an added value of 32.5% in the NPV. The high incidence (33%) of atypical nodules and their frequent malignancy (32%) suggest the widespread employment of Gd-EOB-DTPA-MRI in the follow-up of small nodules ({<=}2 cm) in cirrhosis. (orig.)

  10. Assessment of alveolar epithelial permeability in progressive systemic sclerosis (PSS) using 99mTc-DTPA (diethylene triamine penta acetate) aerosol inhalation

    International Nuclear Information System (INIS)

    To evaluate alveolar epithelial damage in PSS, we studied pulmonary epithelial permeability by measuring the clearance of inhaled 99mTc-DTPA aerosol and performing thin slice CT scan, pulmonary function tests and right heart catheterization in 28 patients with PSS. The 99mTc-DTPA clearance rate (kep value) in PSS was greater than in 11 non-smoking normal subjects (18.2±7.63x10-3/min vs. 9.12±0.77x10-3/min, p2. In contrast, the kep value showed significant correlations with %DLco (diffusing capacity for carbon monoxide), extent of interstitial lesions evaluated by CT scan (CT score), and mean pulmonary artery pressure. On the other hand, the kep value was high in some patients with normal CT scan and normal %DLco. These findings indicate that pulmonary interstitial lesions in PSS are accompanied by alveolar epithelial damage, and that the clearance of 99mTc-DTPA may be an early predictor of interstitial change. (author)

  11. Preferential decorporation of americium by pulmonary administration of DTPA dry powder after inhalation of aged PuO2 containing americium in rats

    International Nuclear Information System (INIS)

    After inhalation of plutonium oxides containing various percentages of americium in rats, we identified an acellular transient pulmonary compartment, the epithelial lining fluid (ELF), in which a fraction of actinide oxides dissolve prior to absorption and subsequent extrapulmonary deposit. Chelation therapy is usually considered to be poorly efficient after inhalation of actinide oxides. However, in the present study, prompt pulmonary administration of diethylenetriaminepentaacetic acid (DTPA) as a dry powder led to a decrease in actinide content in ELF together with a limitation of bone and liver deposits. Because americium is more soluble than plutonium, higher amounts of americium were found in ELF, extrapulmonary tissues and urine. Our results also demonstrated that the higher efficacy of DTPA on americium compared to plutonium in ELF induced a preferential inhibition of extrapulmonary deposit and a greater urinary excretion of americium compared to plutonium. All together, our data justify the use of an early and local DTPA treatment after inhalation of plutonium oxide aerosols in which americium can be in high proportion such as in aged compounds. (authors)

  12. Global detailed gravimetric geoid. [based on gravity model derived from satellite tracking and surface gravity data

    Science.gov (United States)

    Vincent, S.; Marsh, J. G.

    1973-01-01

    A global detailed gravimetric geoid has been computed by combining the Goddard Space Flight Center GEM-4 gravity model derived from satellite and surface gravity data and surface 1 deg-by-1 deg mean free air gravity anomaly data. The accuracy of the geoid is + or - 2 meters on continents, 5 to 7 meters in areas where surface gravity data are sparse, and 10 to 15 meters in areas where no surface gravity data are available. Comparisons have been made with the astrogeodetic data provided by Rice (United States), Bomford (Europe), and Mather (Australia). Comparisons have also been carried out with geoid heights derived from satellite solutions for geocentric station coordinates in North America, the Caribbean, Europe, and Australia.

  13. A Topological derivative based image segmentation for sign language recognition system using isotropic filter

    CERN Document Server

    Krishnaveni, M

    2010-01-01

    The need of sign language is increasing radically especially to hearing impaired community. Only few research groups try to automatically recognize sign language from video, colored gloves and etc. Their approach requires a valid segmentation of the data that is used for training and of the data that is used to be recognized. Recognition of a sign language image sequence is challenging because of the variety of hand shapes and hand motions. Here, this paper proposes to apply a combination of image segmentation with restoration using topological derivatives for achieving high recognition accuracy. Image quality measures are conceded here to differentiate the methods both subjectively as well as objectively. Experiments show that the additional use of the restoration before segmenting the postures significantly improves the correct rate of hand detection, and that the discrete derivatives yields a high rate of discrimination between different static hand postures as well as between hand postures and the scene b...

  14. Highly selective fluorescent chemosensor for Na+ based on pyrene-modified calix[4]arene derivative

    Institute of Scientific and Technical Information of China (English)

    WANG KeRang; GUO DongSheng; JIANG BangPing; LIU Yu

    2009-01-01

    A novel calix[4]arene derivative with pyrene fluorophores at the upper rim and tetraester ionophores at the lower rim was synthesized in six steps, and its structure was proved by NMR and ESi-MS spectro-scopies. Furthermore, the chemosensing behavior of the host compound for alkali and alkaline earth metal ions was investigated by fluorescence spectroscopy. The obtained results show that the calix-arene host can selectively bind sodium ion with the complexation stability constant of 2190 mol-1.L. The complexation with sodium ion can pronouncedly induce the excimer emission to decrease and the monomer emission to increase, whereas the addition of the other alkali and alkaline earth metal ions does not cause appreciable changes in the fluorescence spectrum of the host compound. The present calix[4]arene derivative displays potential application as fluorescent chemosensor for sodium ion.

  15. Highly selective fluorescent chemosensor for Na~+ based on pyrene-modified calix[4]arene derivative

    Institute of Scientific and Technical Information of China (English)

    2009-01-01

    A novel calix[4]arene derivative with pyrene fluorophores at the upper rim and tetraester ionophores at the lower rim was synthesized in six steps,and its structure was proved by NMR and ESI-MS spectro-scopies. Furthermore,the chemosensing behavior of the host compound for alkali and alkaline earth metal ions was investigated by fluorescence spectroscopy. The obtained results show that the calix-arene host can selectively bind sodium ion with the complexation stability constant of 2190 mol-1.L. The complexation with sodium ion can pronouncedly induce the excimer emission to decrease and the monomer emission to increase,whereas the addition of the other alkali and alkaline earth metal ions does not cause appreciable changes in the fluorescence spectrum of the host compound. The present calix[4]arene derivative displays potential application as fluorescent chemosensor for sodium ion.

  16. Highly Selective Fluoride Recognition by a Simple Carbazole-based Hydrozone Derivative

    Institute of Scientific and Technical Information of China (English)

    TANG Li-jun; ZHAO Guo-you; HUANG Zhen-long; WANG Nan-nan; GUO Jiao-jiao

    2013-01-01

    A simple carbazole hydrozone derivative(1) was synthesized and used as an optical probe for fluoride recognition in CH3CN.Receptor 1 exhibited colorimetric and fluorescent dual-channel response to fluoride anions.Addition of fluoride to a receptor 1 solution in CH3CN induced a distinct color change from yellow to magenta,the solution also displayed significant fluorescence blue shift.Thus receptor 1 exhibited dual-channel responses to fluoride with a high selectivity and sensitivity.

  17. Simulation of air shower image in fluorescence light based on energy deposits derived from CORSIKA

    OpenAIRE

    Gora, D.; Heck, D.; Homola, P.; Klages, H.; Pekala, J.; Risse, M.; Wilczynska, B.; Wilczynski, H.

    2004-01-01

    Spatial distributions of energy deposited by an extensive air shower in the atmosphere through ionization, as obtained from the CORSIKA simulation program, are used to find the fluorescence light distribution in the optical image of the shower. The shower image derived in this way is somewhat smaller than that obtained from the NKG lateral distribution of particles in the shower. The size of the image shows a small dependence on the primary particle type.

  18. Derivation of Exact Eigenvalues and Eigenfunctions Based on the Analytical Transfer Matrix Method

    Institute of Scientific and Technical Information of China (English)

    HE Ying; CAO Zhuang-Qi; SHEN Qi-Shun

    2004-01-01

    @@ We extend the analytical transfer matrix method (ATMM) to calculate both the energy eigenvalues and wavefunctions for the Morse potential and the regulated Coulomb potential. Derivations of the exact eigenenergies and eigenfunctions are presented in detail by the ATMM. We compare our results with that obtained by relaxational approach and the eigenvalue moment method, and it is shown that the ATMM can produce accurate eigenvalues.The eigenfunctions by the ATMM are also proven to be correct and meaningful.

  19. ROBUST POSITIONING OF LASER BEAMS USING PROPORTIONAL INTEGRAL DERIVATIVE AND BASED OBSERVER-FEEDBACK CONTROL

    OpenAIRE

    Kwabena A. Konadu; Sun Yi; Wonchang Choi; Taher Abu-Lebdeh

    2013-01-01

    High-precision positioning of laser beams has been a great challenge in industry due to inevitable existence of noise and disturbance. The work presented in this study addresses this problem by employing two different control strategies: Proportional Integral Derivative (PID) control and state feedback control with an observer. The control strategies are intended to stabilize the position of a laser beam on a Position Sensing Device (PSD) located on a Laser Beam Stabilization (or, laser beam ...

  20. Activity Coefficient Derivatives of Ternary Systems Based on Scatchard's Neutral Electrolyte description

    Energy Technology Data Exchange (ETDEWEB)

    Miller, D G

    2007-05-16

    Activity coefficient derivatives with respect to molality are presented for the Scatchard Neutral Electrolyte description of a ternary common-ion electrolyte system. These quantities are needed for the calculation of 'diffusion Onsager coefficients' and in turn for tests of the Onsager Reciprocal Relations in diffusion. The usually-omitted b{sub 23} term is included. The direct SNE binary approximations and a further approximation are discussed. Binary evaluation strategies other than constant ionic strength are considered.