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Sample records for based dtpa derivative

  1. Design and synthesis of novel adenine fluorescence probe based on Eu(III) complexes with dtpa-bis(guanine) ligand.

    Science.gov (United States)

    Tian, Fengyun; Jiang, Xiaoqing; Dou, Xuekai; Wu, Qiong; Wang, Jun; Song, Youtao

    2017-02-24

    A novel adenine (Ad) fluorescence probe (Eu(III)-dtpa-bis(guanine)) was designed and synthesized by improving experimental method based on the Eu(III) complex and dtpa-bis(guanine) ligand. The dtpa-bis(guanine) ligand was first synthesized by the acylation action between dtpaa and guanine (Gu), and the corresponding Eu(III) complex was successfully prepared through heat-refluxing method with dtpa-bis(guanine) ligand. As a novel fluorescence probe, the Eu(III)-dtpa-bis(guanine) complex can detect adenine (Ad) with characteristics of strong targeting, high specificity and high recognition ability. The detection mechanism of the adenine (Ad) using this probe in buffer solution was studied by ultraviolet-visible (UV-vis) and fluorescence spectroscopy. When the Eu(III)-dtpa-bis(guanine) was introduced to the adenine (Ad) solution, the fluorescence emission intensity was significantly enhanced. However, adding other bases such as guanine (Gu), xanthine (Xa), hypoxanthine (Hy) and uric acid (Ur) with similar composition and structure to that of adenine (Ad) to the Eu(III)-dtpa-bis(guanine) solution, the fluorescence emission intensities are nearly invariable. Meanwhile, the interference of guanine (Gu), xanthine (Xa), hypoxanthine (Hy) and uric acid (Ur) on the detection of the adenine using Eu(III)-dtpa-bis(guanine) probe was also studied. It was found that presence of these bases does not affect the detection of adenine (Ad). A linear response of fluorescence emission intensities of Eu(III)-dtpa-bis(guanine) at 570nm as a function of adenine (Ad) concentration in the range of 0.00-5.00×10(-5)molL(-1) was observed. The detection limit is about 4.70×10(-7)molL(-1).

  2. Volume-assisted estimation of liver function based on Gd-EOB-DTPA-enhanced MR relaxometry

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    Haimerl, Michael; Schlabeck, Mona; Verloh, Niklas; Fellner, Claudia; Stroszczynski, Christian; Wiggermann, Philipp [University Hospital Regensburg, Department of Radiology, Regensburg (Germany); Zeman, Florian [University Hospital Regensburg, Center for Clinical Trials, Regensburg (Germany); Nickel, Dominik [MR Applications Development, Siemens AG, Healthcare Sector, Erlangen (Germany); Barreiros, Ana Paula [University Hospital Regensburg, Department of Internal Medicine I, Regensburg (Germany); Loss, Martin [University Hospital Regensburg, Department of Surgery, Regensburg (Germany)

    2016-04-15

    To determine whether liver function as determined by indocyanine green (ICG) clearance can be estimated quantitatively from hepatic magnetic resonance (MR) relaxometry with gadoxetic acid (Gd-EOB-DTPA). One hundred and seven patients underwent an ICG clearance test and Gd-EOB-DTPA-enhanced MRI, including MR relaxometry at 3 Tesla. A transverse 3D VIBE sequence with an inline T1 calculation was acquired prior to and 20 minutes post-Gd-EOB-DTPA administration. The reduction rate of T1 relaxation time (rrT1) between pre- and post-contrast images and the liver volume-assisted index of T1 reduction rate (LVrrT1) were evaluated. The plasma disappearance rate of ICG (ICG-PDR) was correlated with the liver volume (LV), rrT1 and LVrrT1, providing an MRI-based estimated ICG-PDR value (ICG-PDR{sub est}). Simple linear regression model showed a significant correlation of ICG-PDR with LV (r = 0.32; p = 0.001), T1{sub post} (r = 0.65; p < 0.001) and rrT1 (r = 0.86; p < 0.001). Assessment of LV and consecutive evaluation of multiple linear regression model revealed a stronger correlation of ICG-PDR with LVrrT1 (r = 0.92; p < 0.001), allowing for the calculation of ICG-PDR{sub est}. Liver function as determined using ICG-PDR can be estimated quantitatively from Gd-EOB-DTPA-enhanced MR relaxometry. Volume-assisted MR relaxometry has a stronger correlation with liver function than does MR relaxometry. (orig.)

  3. Chitosan oligosaccharide based Gd-DTPA complex as a potential bimodal magnetic resonance imaging contrast agent.

    Science.gov (United States)

    Huang, Yan; Cao, Juan; Zhang, Qi; Lu, Zheng-rong; Hua, Ming-qing; Zhang, Xiao-yan; Gao, Hu

    2016-01-01

    A new gadolinium diethylenetriamine pentaacetic acid (DTPA) complex (Gd-DTPA-DMABA-CS11) as a potential bimodal magnetic resonance imaging (MRI) contrast agent with fluorescence was synthesized. It was synthesized by the incorporation of 4-dimethylaminobenzaldehyde (DMABA) and chitosan oligosaccharide (CSn; n=11) with low polydispersity index to DTPA anhydride and then chelated with gadolinium chloride. The structure was characterized by Fourier transform infrared (FTIR), (1)H NMR, elemental analysis and size exclusion chromatography (SEC). MRI measurements in vitro were evaluated. The results indicated that Gd-DTPA-DMABA-CS11 provided higher molar longitudinal relaxivity (r1) (12.95mM(-1)·s(-1)) than that of commercial Gd-DTPA (3.63mM(-1)·s(-1)) at 0.5T. Gd-DTPA-DMABA-CS11 also emitted fluorescence, and the intensity was much stronger than that of Gd-DTPA. Therefore, it can be meanwhile used in fluorescent imaging for improving the sensitivity in clinic diagnosis. Gd-DTPA-DMABA-CS11 as a potential contrast agent is preliminarily stable in vitro. The results of thermodynamic action between Gd-DTPA-DMABA-CS11 and bovine serum albumin (BSA) illustrated that the binding process was exothermic and spontaneous, and the main force was van der Waals' interaction and hydrogen bond. The preliminary study suggested that Gd-DTPA-DMABA-CS11 could be used in both magnetic resonance and fluorescent imaging as a promising bimodal contrast agent.

  4. Development and biodistrubition modeling of 99mTc-DTPA

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    Mohammed Bricha

    2015-09-01

    Full Text Available Purpose: In this study, the team modeled the biodistribution and the efficiency of two 99m-technetium diethylene triamine penta acetate (99mTc-DTPA based radiopharmaceuticals.Methods: The first radiopharmaceutical (DTPA-CNESTEN is developed at the laboratories of the radiopharmaceutical production unit of the National Center for Nuclear Energy, Sciences and Technologies (CNESTEN-Morocco, and the second one is the commercial DTPA (DTPA-ref. Freeze-dried kits were successfully radiolabeled (radiochemical purity >95% with the 99m Tc. Then drugs were injected to male BALB/c mice. In each 2 min, 5 min, 15 min, 1 h and 2 h time points after injections we evaluate tissue’s distributions characteristics. At the end, an automatic modeling of the data were recorded from thyroid, blood and urinary excretion kinetics and biodistribution in mice using both DTPA kits. The study aimed to extract the parameters of the function used to fit the recorded data. Results and Conclusion: the team concluded that the biodistribution of 99mTc-DTPA can be modeled using a combination of two exponential parts. Moreover, the resultant plots showed that there is strong correlation between the formula found in literature and the one derived on the basis of the fit of data sets in this study. In addition, it was found that the biodistribution behaviors of the developed kit and the commercial one were very close. The obtained results suggest that the developed DTPA has practically the same kinetics as the commercial one.

  5. Synthesis, Relaxivity and MRI Enhancement of Linear Oligo-Gd(m) Complexes with Poly (DTPA-ester) Ligands Derived from Amino Acids

    Institute of Scientific and Technical Information of China (English)

    YU,Kai-Chao (俞开潮); WANG,Xin-Bing(王新兵); YE,Chao-Hui(叶朝辉); LI,Li-Yun(李丽云); LIU,Mai-Li(刘买利); ZHUO,Ren-Xi(卓仁禧)

    2001-01-01

    Six linear oligo-DrPA-ester Gd(Ⅲ) conplexes being used for potential MRI contrast agents were synthesized from amino acids and characterized. Their longitudinal relaxation rates were measured. One of them, the pheny derivative, with high rciaxivity, was chosen for the acute toxicity and T1weighted imaging test. The results indicated that there was no obvious toxicity for fhis new oligomeric Gd(Ⅲ) complex, and it exhibits the highly enhanced MRI signal intensity and the increasing signal duration in the liver tissue conpared to Gd-DTPA.

  6. Design, synthesis and evaluation of a new Mn - Contrast agent for MR imaging of myocardium based on the DTPA-phenylpentadecanoic acid complex

    Science.gov (United States)

    Belyanin, Maxim L.; Stepanova, Elena V.; Valiev, Rashid R.; Filimonov, Victor D.; Usov, Vladimir Y.; Borodin, Oleg Y.; Ågren, Hans

    2016-11-01

    In the present paper we describe the first synthesis and evaluation of a novel Mn (II) complex (DTPA-PPDA Mn (II)) which contains a C-15 fatty acid moiety that has high affinity to the heart muscle. The complexation energy of DTPA-PPDA Mn (II) evaluated by quantum chemistry methodology indicates that it essentially exceeds the corresponding value for the known DTPA Mn (II) complex. Molecular docking revealed that the affinity of the designed complex to the heart-type transport protein H-FABP well exceeds that of lauric acid. Phantom experiments in low-field MRI the designed contrast agent provides MR imaging comparable to gadopentetic acid.

  7. Anti-diphtheria antibody seroprotection rates are similar 10 years after vaccination with dTpa or DTPa using a mathematical model.

    Science.gov (United States)

    Cheuvart, Brigitte; Burgess, Margaret; Zepp, Fred; Mertsola, Jussi; Wolter, Joanne; Schuerman, Lode

    2004-12-01

    The reduced antigen content diphtheria, tetanus and pertussis (dTpa) vaccine (Boostrixtrade mark) has been shown to induce a strong booster response to all the vaccine components in 4-6 year olds. However, anti-diphtheria antibody levels were observed to be lower when compared to the "full strength" paediatric DTPa vaccine. To assess the impact of this difference on long-term protection, a mathematical model was developed to predict diphtheria antibody decay over time. The model was based on a linear decrease in log-transformed antibody concentrations after the first year post-vaccination. When applied to data collected 3.5 years after vaccination of 4-6 year olds with either DTPa or dTpa, the model predicted that 10 years post-vaccination, 98.6% of subjects vaccinated with dTpa were likely to remain seroprotected against diphtheria, compared to 99.6% vaccinated with DTPa. Therefore, the difference observed in diphtheria antibody geometric mean concentrations 1 month after booster vaccination at 4-6 years with dTpa or DTPa is unlikely to be of clinical relevance 10 years later at the time of the adolescent booster.

  8. Influence of DTPA Treatment on Internal Dose Estimates.

    Science.gov (United States)

    Davesne, Estelle; Blanchardon, Eric; Peleau, Bernadette; Correze, Philippe; Bohand, Sandra; Franck, Didier

    2016-06-01

    In case of internal contamination with plutonium materials, a treatment with diethylene triamine pentaacetic acid (DTPA) can be administered in order to reduce plutonium body burden and consequently avoid some radiation dose. DTPA intravenous injections or inhalation can start almost immediately after intake, in parallel with urinary and fecal bioassay sampling for dosimetric follow-up. However, urine and feces excretion will be significantly enhanced by the DTPA treatment. As internal dose is calculated from bioassay results, the DTPA effect on excretion has to be taken into account. A common method to correct bioassay data is to divide it by a factor representing the excretion enhancement under DTPA treatment by intravenous injection. Its value may be based on a nominal reference or observed after a break in the treatment. The aim of this study was to estimate the influence of this factor on internal dose by comparing the dose estimated using default or upper and lower values of the enhancement factor for 11 contamination cases. The observed upper and lower values of the enhancement factor were 18.7 and 63.0 for plutonium and 24.9 and 28.8 for americium. For americium, a default factor of 25 is proposed. This work demonstrates that the use of a default DTPA enhancement factor allows the determination of the magnitude of the contamination because dose estimated could vary by a factor of 2 depending on the value of the individual DTPA enhancement factor. In case of significant intake, an individual enhancement factor should be determined to obtain a more reliable dose assessment.

  9. Complexation of curium(III) with DTPA at 10-70 °C: comparison with Eu(III)-DTPA in thermodynamics, luminescence, and coordination modes.

    Science.gov (United States)

    Tian, Guoxin; Zhang, Zhiyong; Martin, Leigh R; Rao, Linfeng

    2015-02-16

    Separation of trivalent actinides (An(III)) from trivalent lanthanides (Ln(III)) is a challenging task because of the nearly identical chemical properties of these groups. Diethylenetriaminepentaacetate (DTPA), a key reagent used in the TALSPEAK process that effectively separates An(III) from Ln(III), is believed to play a critical role in the An(III)/Ln(III) separation. However, the underlying principles for the separation based on the difference in the complexation of DTPA with An(III) and Ln(III) remain unclear. In this work, the complexation of DTPA with Cm(III) at 10-70 °C was investigated by spectrophotometry, luminescence spectroscopy, and microcalorimetry, in conjunction with computational methods. The binding strength, the enthalpy of complexation, the coordination modes, and the luminescence properties are compared between the Cm(III)-DTPA and Eu(III)-DTPA systems. The experimental and computational data demonstrated that the difference between Cm(III) and Eu(III) in the binding strength with DTPA can be attributed to the stronger covalence bonding between Cm(III) and the nitrogen donors of DTPA.

  10. Preclinical evaluation of Gd-DTPA and gadomelitol as contrast agents in DCE-MRI of cervical carcinoma interstitial fluid pressure

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    Hompland Tord

    2012-11-01

    Full Text Available Abstract Background High interstitial fluid pressure (IFP in the primary tumor is associated with poor disease-free survival in locally advanced cervical carcinoma. A noninvasive assay is needed to identify cervical cancer patients with highly elevated tumor IFP because these patients may benefit from particularly aggressive treatment. It has been suggested that dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI with gadolinium diethylene-triamine penta-acetic acid (Gd-DTPA as contrast agent may provide useful information on the IFP of cervical carcinomas. In this preclinical study, we investigated whether DCE-MRI with contrast agents with higher molecular weights (MW than Gd-DTPA would be superior to Gd-DTPA-based DCE-MRI. Methods CK-160 human cervical carcinoma xenografts were subjected to DCE-MRI with Gd-DTPA (MW of 0.55 kDa or gadomelitol (MW of 6.5 kDa as contrast agent before tumor IFP was measured invasively with a Millar SPC 320 catheter. The DCE-MRI was carried out at a spatial resolution of 0.23 × 0.23 × 2.0 mm3 and a time resolution of 14 s by using a 1.5-T whole-body scanner and a slotted tube resonator transceiver coil constructed for mice. Parametric images were derived from the DCE-MRI recordings by using the Tofts iso-directional transport model and the Patlak uni-directional transport model. Results When gadomelitol was used as contrast agent, significant positive correlations were found between the parameters of both pharmacokinetic models and tumor IFP. On the other hand, significant correlations between DCE-MRI-derived parameters and IFP could not be detected with Gd-DTPA as contrast agent. Conclusion Gadomelitol is a superior contrast agent to Gd-DTPA in DCE-MRI of the IFP of CK-160 cervical carcinoma xenografts. Clinical studies attempting to develop DCE-MRI-based assays of the IFP of cervical carcinomas should involve contrast agents with higher MW than Gd-DTPA.

  11. Degeneration of uterine leiomyoma: comparison between Gd-DTPA enhanced MR imaging and pathologic findings

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    Shin, M. J.; Kim, G. W.; No, T. Y.; Ahn, W. H.; Baik, S. K.; Kim, B. G.; Choi, H. Y.; Paik, O. J. [Wallace Memorial Baptist Hospital, Busan (Korea, Republic of)

    1995-05-15

    Gonadotropin-releasing hormone(GnRH) analogues have been used as a conservative or preoperative therapy in the treatment of uterine leiomyoma. Since these drugs, which can induce a low estrogenic state, affect only undegenerative leiomyoma tissue, the aim of the present study was to differentiate degenerative leiomyoma from undegenerative one by Gd-DTPA enhanced magnetic resonance imaging(MRI). There were 40 masses in 24 patients; all were diagnosed by surgical-pathology. With superconductive 0.5T MR circuit, T1-and T2-weighted images and Gd-DTPA T1-weighted images were obtained. Based on a combination of signal intensities of T2-weighted and enhanced T1 weighted image on the same tumor section all the lesions were classified to one of four MRI patterns. Pattern I was a heterogeneous hyperintensity on the T2-weighted images that was enhancement by Gd-DTPA. Pattern II was a high signal intensity on the T2-weighted MR images but no enhancement by Gd-DTPA. Pattern III was a low to intermediate signal intensity on the T2-weighted MR images and enhancement on the Gd-DTPA T1-weighted images. Pattern IV was a low to intermediate signal intensity on the T2-weighted images and no enhancement by Gd-DTPA. Each of these 4 groups of MRI pattern were co-related to the surgical-pathology findings. Pathologically, pattern I corresponded to an edematous leiomyoma in 3 cases and a connective tissue leiomyoma in one case. Pattern II corresponded to three red degeneration, two cystic degeneration and one infected leiomyoma. Pattern III corresponded to eighteen undegenerative leiomyomas, but some of them showed focal connective tissue proliferation. All of twelve cases in pattern IV corresponded to a hyaline degeneration. MRI with Gd-DTPA enhancement may differentiate undegenerative leiomyoma from degenerated one.

  12. Application of metal-free triazole formation in the synthesis of cyclic RGD-DTPA conjugates.

    NARCIS (Netherlands)

    Berkel, S.S. van; Dirks, A.T.; Meeuwissen, S.A.; Pingen, D.L.L.; Boerman, O.C.; Laverman, P.; Delft, F.L. van; Cornelissen, J.J.; Rutjes, F.P.J.T.

    2008-01-01

    The tandem 1,3-dipolar cycloaddition-retro-Diels-Alder (tandem crDA) reaction is presented as a versatile method for metal-free chemoselective conjugation of a DTPA radiolabel to N-delta-azido-cyclo(-Arg-Gly-Asp-d-Phe-Orn-) via oxanorbornadiene derivatives. To this end, the behavior of several trifl

  13. Evaluation of a new DTPA-derivative chelator: comparative biodistribution and imaging studies of [sup 111]In-labeled B3 monoclonal antibody in athymic mice bearing human epidermoid carcinoma xenografts. [Diethylenetriaminpentaacetic acid

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    Camera, L.; Kinuya, S.; Garmestani, K.; Pai, L.H.; Brechbiel, M.W.; Gansow, O.A.; Paik, C.H.; Pastan, I.; Carrasquillo, J.A. (National Cancer Inst., Bethesda, MD (United States))

    1993-11-01

    Biodistribution and imaging characteristics of monoclonal antibody (MAb) B3 conjugated to either the 2-(p-isothiocvanatobenzyl)-cyclohexyl-DTPA (CHX-B) or 2-(p-isothiocyanatobenzyl)-6-methyl-DTPA (1B4M) and labeled with [sup 111]In, were evalulated in nude mice bearing A431 human epidermoid carcinoma xenografts. MAb B3, is a murine IgG1k reacting with a carbohydrate antigen abundantly expressed by most carcinomas. Both [sup 111]In-(CHX-B)-B3 and [sup 111]In-(1B4M)-B3 showed good tumor targeting with peak values observed at 72 h with 27.6 [+-] 7.6 and 25.4 [+-] 1.7% ID/g, respectively (P > 0.05). High tumor-to-organ ratios were also observed and, confirmed by the imaging results. In particular, tumor-to liver ratios increased from 5.0 [+-] 0.9 at 24 h to 9.2 [+-] 2.0 at 168 h for [sup 111]In-(CHX-B)-B3 and from 4.5 [+-] 0.6 to 8.9 [+-] 3.5 for [sup 111]In-(1B4M)-B3. This was mainly the result of low liver accumulation of both [sup 111]In-(CHX-B)-B3 and [sup 111]In-(1B4M)-B3, with only 2.48 [+-] 0.46 and 2.5 [+-] 0.9% ID/g at 168h, respectively (P > 0.05). Our findings indicate that either CHX-B or 1B4M can be successfully used for [sup 111]In-labeling of MAbs and that [sup 111]In-B3 may represent a promising radioimmunoimaging agent. (Author).

  14. Imaging-based evaluation of liver function: comparison of {sup 99m}Tc-mebrofenin hepatobiliary scintigraphy and Gd-EOB-DTPA-enhanced MRI

    Energy Technology Data Exchange (ETDEWEB)

    Geisel, Dominik; Gebauer, Bernhard [Charite Campus Virchow-Klinikum, Department of Diagnostic and Interventional Radiology, Berlin (Germany); Luedemann, Lutz [Essen University Hospital, Department of Medical Physics, Essen (Germany); Froeling, Vera; Denecke, Timm [Charite Campus Virchow-Klinikum, Department of Diagnostic and Interventional Radiology, Berlin (Germany); Charite Campus Virchow-Klinikum, Department of Nuclear Medicine, Berlin (Germany); Malinowski, Maciej; Stockmann, Martin; Baron, Annekathrin; Seehofer, Daniel [Charite Campus Virchow-Klinikum, Department of General, Visceral and Thoracic Surgery, Berlin (Germany); Prasad, Vikas [Charite Campus Virchow-Klinikum, Department of Nuclear Medicine, Berlin (Germany)

    2015-05-01

    To compare Gd-EOB-enhanced MRI and {sup 99m}Tc-mebrofenin hepatobiliary scintigraphy (HBS) as imaging-based liver function tests for separate evaluation of right (RLL) and left liver lobe (LLL) function. Fourteen patients underwent Gd-EOB-enhanced MRI and {sup 99m}Tc-mebrofenin HBS after portal vein embolization within 24 h. Relative enhancement (RE) and hepatic uptake index (HUI) were determined from MRI; and T{sub max}, T{sub 1/2} and mebrofenin uptake were determined from HBS, all values separately for RLL and LLL. Mebrofenin uptake correlated significantly with HUI and RE for both liver lobes. There was strong correlation of mebrofenin uptake with HUI for RLL (r{sup 2} = 0.802, p = 0.001) and RE for LLL (r{sup 2} = 0.704, p = 0.005) and moderate correlation with HUI for LLL (r{sup 2} = 0.560, p = 0.037) and RE for RLL (r{sup 2} = 0.620, p = 0.018). Correlating the percentage share of RLL function derived from MRI (with HUI) with the percentage of RLL function derived from mebrofenin uptake revealed a strong correlation (r{sup 2} = 0.775, p = 0.002). Both RE and HUI correlate with mebrofenin uptake in HBS. The results suggest that Gd-EOB-enhanced MRI and {sup 99m}Tc-mebrofenin HBS may equally be used to separately determine right and left liver lobe function. (orig.)

  15. CanthebiliaryenhancementofGd-EOB-DTPA predictthedegreeofliverfunction?

    Institute of Scientific and Technical Information of China (English)

    Masahiro Okada; Kazunari Ishii; Kazushi Numata; Tomoko Hyodo; Seishi Kumano; Masayuki Kitano; Masatoshi Kudo; Takamichi Murakami

    2012-01-01

    BACKGROUND: Excretion of gadolinium-ethoxybenzyl-diethyl-enetriamine pentaacetic acid (Gd-EOB-DTPA) in the bile may be related to liver function, because of elimination from the liver after preferential uptake by hepatocytes. The purpose of this study was to investigate the relation between liver and biliary enhancement in patients with or without liver dysfunction, and to compare the tumor-to-liver contrast in these patients. METHODS: Forty patients [group 1: normal liver and Child-Pugh class A in 20 patients, group 2:Child-Pugh class B in 18 patients and Child-Pugh C in 2] were evaluated. All patients underwent MR imaging of the liver using a 1.5-Tesla system. T1-weighted 3D images were obtained at 5, 10, 15 and 20 minutes after Gd-EOB-DTPA injection. The relation between group 3 (total bilirubin RESULTS:Liver enhancement showed signiifcant difference at all time points between groups 1 and 2. Biliary enhancement did not show a signiifcant difference between groups 1 and 2 at 5 minutes, but did at 10, 15 and 20 minutes. At 20 minutes, signiifcant differences between groups 3 and 4 were seen for liver and biliary enhancement. At all time points, liver enhancement correlated with biliary enhancement in both groups. At 5 minutes and 20 minutes, statistical differences between groups 1 and 2 were seen for tumor-to-liver ratio. CONCLUSIONS: The degree of biliary enhancement has a close correlation to that of liver enhancement. It is especially important that insufifcient liver enhancement causes lower tumor-to-liver contrast in the hepatobiliary phase of Gd-EOB-DTPA.

  16. Financial Derivatives (Based on Two Supports Evaluation

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    Tiberiu Socaciu

    2011-10-01

    Full Text Available In this paper we build a PDE like Black-Scholes equation in hypothesis of a financial derivative that is dependent on two supports (usual is dependent only on one support, like am
    option based on gold, when national currency has a great float.
    Keywords: Financial derivatives, derivatives evaluation, derivatives based on two supports, extended Itō like lemma.

  17. View-based Propagator Derivation

    CERN Document Server

    Schulte, Christian

    2009-01-01

    When implementing a propagator for a constraint, one must decide about variants: When implementing min, should one also implement max? Should one implement linear constraints both with unit and non-unit coefficients? Constraint variants are ubiquitous: implementing them requires considerable (if not prohibitive) effort and decreases maintainability, but will deliver better performance than resorting to constraint decomposition. This paper shows how to use views to derive perfect propagator variants. A model for views and derived propagators is introduced. Derived propagators are proved to be indeed perfect in that they inherit essential properties such as correctness and domain and bounds consistency. Techniques for systematically deriving propagators such as transformation, generalization, specialization, and type conversion are developed. The paper introduces an implementation architecture for views that is independent of the underlying constraint programming system. A detailed evaluation of views implement...

  18. Separation and characterization of the two diastereomers for [Gd(DTPA-bz-NH2)(H2O)]2-, a common synthon in macromolecular MRI contrast agents: their water exchange and isomerization kinetics.

    Science.gov (United States)

    Burai, László; Tóth, Eva; Sour, Angélique; Merbach, André E

    2005-05-16

    Chiral, bifunctional poly(amino carboxylate) ligands are commonly used for the synthesis of macromolecular, Gd(III)-based MRI contrast agents, prepared in the objective of increasing relaxivity or delivering the paramagnetic Gd(III) to a specific site (targeting). Complex formation with such ligands results in two diastereomeric forms for the complex which can be separated by HPLC. We demonstrated that the diastereomer ratio for Ln(III) DTPA derivatives (approximately 60:40) remains constant throughout the lanthanide series, in contrast to Ln(III) EPTPA derivatives, where it varies as a function of the cation size with a maximum for the middle lanthanides (DTPA(5-) = diethylenetriaminepentaacetate; EPTPA(5-) = ethylenepropylenetriaminepentaacetate). The interconversion of the two diastereomers, studied by HPLC, is a proton-catalyzed process (k(obs) = k(1)[H(+)]). It is relatively fast for [Gd(EPTPA-bz-NH(2))(H(2)O)](2-) but slow enough for [Gd(DTPA-bz-NH(2))(H(2)O)](2-) to allow investigation of pure individual isomers (isomerization rate constants are k(1) = (3.03 +/- 0.07) x 10(4) and 11.6 +/- 0.5 s(-1) M(-1) for [Gd(EPTPA-bz-NH(2))(H(2)O)](2)(-) and [Gd(DTPA-bz-NH(2))(H(2)O)](2-), respectively). Individual water exchange rates have been determined for both diastereomers of [Gd(DTPA-bz-NH(2))(H(2)O)](2-) by a variable-temperature (17)O NMR study. Similarly to Ln(III) EPTPA derivatives, k(ex) values differ by a factor of 2 (k(ex)(298) = (5.7 +/- 0.2) x 10(6) and (3.1 +/- 0.1) x 10(6) s(-1)). This variance in the exchange rate has no consequence on the proton relaxivity of the two diastereomers, since it is solely limited by fast rotation. However, such difference in k(ex) will affect proton relaxivity when these diastereomers are linked to a slowly rotating macromolecule. Once the rotation is optimized, slow water exchange will limit relaxivity; thus, a factor of 2 in the exchange rate can lead to a remarkably different relaxivity for the diastereomer complexes

  19. Plutonium-DTPA Model Application with USTUR Case 0269.

    Science.gov (United States)

    Konzen, Kevin; Brey, Richard; Miller, Scott

    2016-01-01

    A plutonium-DTPA (Pu-DTPA) biokinetic model was introduced that had originated from the study of a plutonium-contaminated wound. This work evaluated the extension of the Pu-DTPA model to United States Transuranium and Uranium Registry (USTUR) Case 0269 involving an acute inhalation of a plutonium nitrate aerosol. Chelation was administered intermittently for the first 7 mo as Ca-EDTA, mostly through intravenous injection, with Ca-DTPA treatments administered approximately 2.5 y post intake. Urine and fecal bioassays were collected following intake for several years. Tissues were collected and analyzed for plutonium content approximately 38 y post intake. This work employed the Pu-DTPA model for predicting the urine and fecal bioassay and final tissue quantity at autopsy. The Pu-DTPA model was integrated with two separate plutonium systemic models (i.e., ICRP Publication 67 and its proposed modification). This work illustrated that the Pu-DTPA model was useful for predicting urine and fecal bioassay, including final tissue quantity, 38 y post intake.

  20. DTPA complexation of bismuth in human blood serum.

    Science.gov (United States)

    Montavon, G; Le Du, A; Champion, J; Rabung, T; Morgenstern, A

    2012-07-28

    The in vivo(212)Pb/(212)Bi generator is promising for application in targeted alpha therapy (TAT) of cancer. One main limitation of its therapeutic application is due to potential release of (212)Bi from the radioconjugate upon radioactive decay of the mother nuclide (212)Pb, potentially leading to irradiation of healthy tissue. The objective of the present work is to assess whether the chelate CHX-A''-DTPA (N-(2-aminoethyl)-trans-1,2-diaminocyclohexane-N,N',N''-pentaacetic acid) bound to a biological carrier molecule may be able to re-complex released (212)Bi under in vivo conditions to limit its translocation from the target site. CHX-A''-DTPA was bound to bovine gamma globulin (BGG) to mimic a model conjugate and the stability of the Bi-CHX-A''-DTPA-BGG conjugate was studied in blood serum by ultrafiltration. TRLFS experiments using Cm(III) as a fluorescent probe demonstrated that linking CHX-A''-DTPA to BGG does not affect the coordination properties of the ligand. Furthermore, comparable stability constants were observed between Bi(III) and free CHX-A''-DTPA, BGG-bound CHX-A''-DTPA and DTPA. The complexation constants determined between Bi(III) and the chelate molecules are sufficiently high to allow ultra trace amounts of the ligand to efficiently compete with serum transferrin controlling Bi(III) speciation in blood plasma conditions. Nevertheless, CHX-A''-DTPA is not able to complex Bi(III) generated in blood serum because of the strong competition between Bi(III) and Fe(II) for the ligand. In other words, CHX-A''-DTPA is not "selective" enough to limit Bi(iii) release in the body when applying the (212)Pb/(212)Bi in vivo generator.

  1. Applications for Gd-DTPA in magnetic resonance imaging

    Energy Technology Data Exchange (ETDEWEB)

    Schmidt, H.; Bauer, M.; Heywang, S.; Tiling, R.; Vogl, T.; Yousry, T.

    1988-03-01

    In this article, the physics of paramagnetic contrast agents shortly will be reviewed and useful applications of Gd-DTPA in the CNS, head and neck, abdomen and pelvis will be described. In the CNS Gd-DTPA improves the sensitivity of MRI for small cerebral lesions allows a better delineation between solid tumor, oedema and necrosis. An important indication is the assessment of the extent of intramedullary tumors and the differentiation between cyst and tumor. For the diagnosis of intrameatal acoustic neuromas MRI with Gd-DTPA is the method of choice. Other useful applications are lesions of head and neck and difficult cases for mamography.

  2. Evaluation of cell tracking effects for transplanted mesenchymal stem cells with jetPEI/Gd-DTPA complexes in animal models of hemorrhagic spinal cord injury.

    Science.gov (United States)

    Liu, Yu; He, Zhi-Jie; Xu, Bo; Wu, Qi-Zhu; Liu, Gang; Zhu, Hongyan; Zhong, Qian; Deng, David Y; Ai, Hua; Yue, Qiang; Wei, Yi; Jun, Shen; Zhou, Guangqian; Gong, Qi-Yong

    2011-05-19

    Cell tracking using iron oxide nanoparticles has been well established in MRI. However, in experimental rat models, the intrinsic iron signal derived from erythrocytes masks the labeled cells. The research evaluated a clinically applied Gd-DTPA for T1-weighted positive enhancement for cell tracking in spinal cord injury (SCI) rat models. MSCs were labeled with jetPEI/Gd-DTPA particles to evaluate the transfection efficiency by MRI in vitro. Differentiation assays were carried out to evaluate the differentiation ability of Gd-DTPA-labeled MSCs. The Gd-DTPA-labeled MSCs were transplanted to rat SCI model and monitored by MRI in vivo. Fluorescence images were taken to confirm the MRI results. Behavior test was assessed with Basso, Beattie, and Bresnahan (BBB) scoring in 6weeks after cell transplantation. The Gd-labeled MSCs showed a significant increase in signal intensity in T1-weighted images. After local transplantation, Gd-DTPA-labeled MSCs could be detected in SCI rat models by the persistent T1-weighted positive enhancement from 3 to 14days. Under electronic microscope, Gd-DTPA/jetPEI complexes were mostly observed in cytoplasm. Fluorescence microscopy examination showed that the Gd-labeled MSCs survived and distributed within the injured spinal cord until 2weeks. The Gd-labeled MSCs were identified and tracked with MRI by cross and sagittal sections. The BBB scores of the rats with labeled MSCs transplantation were significantly higher than those of control rats. Our results demonstrated that Gd-DTPA is appropriate for cell tracking in rat model of SCI, indicating that an efficient and nontoxic label method with Gd-DTPA could properly track MSCs in hemorrhage animal models.

  3. Generalized Derivative Based Kernelized Learning Vector Quantization

    NARCIS (Netherlands)

    Schleif, Frank-Michael; Villmann, Thomas; Hammer, Barbara; Schneider, Petra; Biehl, Michael; Fyfe, Colin; Tino, Peter; Charles, Darryl; Garcia-Osoro, Cesar; Yin, Hujun

    2010-01-01

    We derive a novel derivative based version of kernelized Generalized Learning Vector Quantization (KGLVQ) as an effective, easy to interpret, prototype based and kernelized classifier. It is called D-KGLVQ and we provide generalization error bounds, experimental results on real world data, showing t

  4. Fabrication and Characterization of Gd-DTPA-Loaded Chitosan-Poly(Acrylic Acid) Nanoparticles for Magnetic Resonance Imaging.

    Science.gov (United States)

    Ahmed, Arsalan; Zhang, Chao; Guo, Jian; Hu, Yong; Jiang, Xiqun

    2015-08-01

    Gd-DTPA-loaded chitosan-poly(acrylic acid) nanoparticles (Gd-DTPA@CS-PAA NPs) were formulated based on the reaction system of water-soluble polymer-monomer pairs of acrylic acid in chitosan solution followed by sorption of Gd-DTPA. Morphological investigations revealed the spherical shape of these NPs with about 220 nm particle size. These NPs showed charge reversal characteristic in acidic solution. In vitro and in vivo magnetic characteristics of these NPs were explored to estimate their utilization in targeted enhanced magnetic resonance imaging. Relaxation studies showed that these NPs possessed pH susceptible relaxation properties, which could introduce in vivo-specific distribution of contrast agent. MRI experiment showed that these nanoparticles had better results in contrast enhancement, and the concentration of contrast agent increased in liver and brain with increment in time. Thus, these NPs could maintain in vivo long circulation and high relaxation rate and were suitable agents for magnetic resonance imaging.

  5. Tc-99m DTPA scans in renal allograft rejection and cyclosporine nephrotoxicity

    Energy Technology Data Exchange (ETDEWEB)

    Gedroyc, W.; Taube, D.; Fogleman, I.; Neild, G.; Cameron, S.; Maisey, M.

    1986-11-01

    Renal allograft dysfunction arising from rejection or cyclosporine (CsA) nephrotoxicity can currently only be distinguished reliably by allograft biopsy. We have assessed Technetium (Tc)-99m diethylamine pentacetic acid (DTPA) scanning in 30 CsA-treated patients with allograft dysfunction. Scintigrams were performed during 20 biopsy-proved episodes of rejection and during 14 episodes of CsA nephrotoxicity. These results were compared with the scintigrams of 15 allografts showing stable function. Quantitative indices expressing allograft perfusion (flow index) and function (uptake index) derived from the DTPA scintigrams showed no significant differences between the groups of patients with rejection, CsA nephrotoxicity, or stable or improving function. Similarly, the flow and uptake indices of individual allografts obtained during periods of stable or improving function and then during episodes of dysfunction due to rejection or CsA nephrotoxicity did not significantly change. We conclude that Tc-99m DTPA scintigrams are of limited value in the management of allograft dysfunction in patients immunosuppressed with CsA.

  6. MR-angiography with intravenous administration of Gd-DTPA

    Energy Technology Data Exchange (ETDEWEB)

    Saito, Yoko; Yodono, Hiraku; Tarusawa, Koji; Sasaki, Taisuke; Akimura, Rumiko; Kanehira, Jiro; Takahashi, Satoshi; Takekawa, S.D.

    1989-05-01

    We carried out phase contrast MR-angiography of the lower extremities with intravenous administration of Gd-DTPA. Five healthy male volunteers, 25 to 40 years of age, were examined with a 0.5T MRI unit. We used fast scan (gradient echo) technique and it took about 8 minutes for whole procedure. Images were obtained before and after intravenous injection of Gd-DTPA. Injection dose was 0.1 mmol/kg. In two cases, we got images with variable flip angles. However angles of 30 or 40 degrees were thought to be best on the scan with Gd-DTPA. In three cases, we repeated short time procedures for about 4 minutes each time and continued to check the signal intensities of vessels for as long as one hour. The signal intensities greatly increased soon after administration of Gd-DTPA, and then they gradually decreased, but for as long as 60 minutes after administration they remained much higher than those before administration of Gd-DTPA. MR-angiography with Gd-DTPA was found very useful to demonstrate the peripheral femoral vessels clearly. No significant side effect was noticed in any case. Therefore, this method was thought to be very useful clinically. (author).

  7. Complexation thermodynamics and structural studies of trivalent actinide and lanthanide complexes with DTPA, MS-325 and HMDTPA

    Energy Technology Data Exchange (ETDEWEB)

    Thakur, P.; Choppin, G.R. [Florida State Univ., Tallahassee, FL (United States). Dept. of Chemistry and Biochemistry; Conca, J.L. [RJ Lee Group, Inc., Pasco, WA (United States). Center for Lab. Sciences; Dodge, C.J. [Brookhaven National Laboratory, Upton, NY (United States); Francis, A.J. [Brookhaven National Laboratory, Upton, NY (United States); Pohang Univ. of Science and Technology (Korea, Republic of). Div. of Advanced Nuclear Engineering

    2013-05-01

    The protonation constants of DTPA (diethylenetriaminepentaacetic acid) and two derivatives of DTPA, 1-R(4,4-diphenyl cyclohexyl-phosphonyl-methyl diethylenentriaminepentaacetic acid) (MS-325) and (R)-hydroxymethyl-diethylenentriaminepentaacetic acid (HMDTPA) were determined by potentiometric titration in 0.1 M NaClO{sub 4}. The formation of 1: 1 complexes of Am{sup 3+}, Cm{sup 3+} and Ln{sup 3+} cations with these three ligands were investigated by potentiometric titration with competition by ethylenediaminetetraacetic acid (EDTA) and the solvent extraction method in aqueous solutions of I=0.10 M NaClO{sub 4}. The thermodynamic data of complexation were determined by the temperature dependence of the stability constants and by calorimetry. The complexation is exothermic and becomes weaker with increase in temperature. The complexation strength of these ligands follows the order: DTPA {approx} HMDTPA > MS-325. Eu{sup 3+}/Cm{sup 3+} luminescence, EXAFS (Extended X-ray Absorption Fine Structure) and DFT (Density Functional Theory) calculations suggest that all three ligands are octadentate in the complex. In the complex, M(L){sup 2-} (L = DTPA, MS-325 and HMDTPA). The M{sup 3+} binds via five carboxylates oxygen atoms, three nitrogen atoms, and the complex contains one water of hydration. (orig.)

  8. A feasibility study evaluating the relationship between dose and focal liver reaction in stereotactic ablative radiotherapy for liver cancer based on intensity change of Gd-EOB-DTPA-enhanced magnetic resonance images

    Energy Technology Data Exchange (ETDEWEB)

    Jung, Sang Hoon; Yu, Jeong Il; Park, Hee Chul; Lim, Do Hoon; Han, Young Yih [Dept. of Radiation Oncology, amsung Medical Center, Sungkyunkwan University School of Medicine, Seoul (Korea, Republic of)

    2016-03-15

    In order to evaluate the relationship between the dose to the liver parenchyma and focal liver reaction (FLR) after stereotactic ablative body radiotherapy (SABR), we suggest a novel method using a three-dimensional dose distribution and change in signal intensity of gadoxetate disodium-gadolinium ethoxybenzyl diethylenetriamine pentaacetic acid (Gd-EOB-DTPA)-enhanced magnetic resonance imaging (MRI) hepatobiliary phase images. In our method, change of the signal intensity between the pretreatment and follow-up hepatobiliary phase images of Gd-EOB-DTPA-enhanced MRI was calculated and then threshold dose (TD) for developing FLR was obtained from correlation of dose with the change of the signal intensity. For validation of the method, TDs for six patients, who had been treated for liver cancer with SABR with 45-60 Gy in 3 fractions, were calculated using the method, and we evaluated concordance between volume enclosed by isodose of TD by the method and volume identified as FLR by a physician. The dose to normal liver was correlated with change in signal intensity between pretreatment and follow-up MRI with a median R{sup 2} of 0.935 (range, 0.748 to 0.985). The median TD by the method was 23.5 Gy (range, 18.3 to 39.4 Gy). The median value of concordance was 84.5% (range, 44.7% to 95.9%). Our method is capable of providing a quantitative evaluation of the relationship between dose and intensity changes on follow-up MRI, as well as determining individual TD for developing FLR. We expect our method to provide better information about the individual relationship between dose and FLR in radiotherapy for liver cancer.

  9. Effect of raised thoracic pressure and volume on 99mTc-DTPA clearance in humans

    Energy Technology Data Exchange (ETDEWEB)

    Nolop, K.B.; Maxwell, D.L.; Royston, D.; Hughes, J.M.

    1986-05-01

    Although positive airway pressure is often used to treat acute pulmonary edema, the effects on epithelial solute flux are not well known. We measured independently the effect of 1) positive pressure and 2) voluntary hyperinflation on the clearance of inhaled technetium-99m-labeled diethylenetriaminepentaacetic acid (/sup 99m/Tc-DTPA) in six nonsmokers and six smokers. Lung volumes were monitored by inductance plethysmography. Each subject was studied in four situations: 1) low end-expiratory volume (LO-), 2) low volume plus 9 cmH2O continuous positive airway pressure (LO+), 3) high end-expiratory volume (HI-), and 4) high volume plus continuous positive airway pressure (HI+). The clearance half time of 99mTc-DTPA for the nonsmokers decreased from 64.8 +/- 7.0 min (mean +/- SE) at LO- to 23.2 +/- 5.3 min at HI- (P less than 0.05). Positive pressure had no synergistic effect. The mean clearance half time for the smokers was faster than nonsmokers at base line but unaffected by similar changes in thoracic volume and pressure. We conclude that, in nonsmokers, positive airway pressure increases /sup 99m/Tc-DTPA clearance primarily through an increase in lung volume and that smokers are immune to these effects.

  10. Hemophilic arthropathy of the knee joint: static and dynamic Gd-DTPA - enhanced MRI

    Energy Technology Data Exchange (ETDEWEB)

    Naegele, M. [Dept. of Radiology, Univ. Bonn (Germany); Bruening, R. [Dept. of Radiology, Univ. Muenchen (Germany); Kunze, V. [Dept. of Radiology, Univ. Bonn (Germany); Eickhoff, H. [Dept. of Orthopedic Surgery, Troisdorf (Germany); Koch, W. [Dept. of Orthopedic Surgery, Troisdorf (Germany); Reiser, M. [Dept. of Radiology, Univ. Muenchen (Germany)

    1995-12-31

    A total of 17 patients with hemophilic arthropathy of the knee joint were studied with static and dynamic MRI before and after an IV bolus injection of Gadolinium-DTPA (Gd-DTPA; 0.1 mmol/kg body weight). The T1-weighted spin-echo (SE) and gradient-echo (fast-field echo [FFE]) sequences were applied. The FFE sequences of eight consecutive scans carried out over a time interval of 160 s were used in order to determine the time to signal intensity (SI) curves of the synovial proliferations surrounding soft tissue, bone marrow, and joint effusion. After the administration of a contrast agent, synovial proliferations exhibited an increase on FFE and SE images of 47.7% (SD {+-} 14.3%) and 37.4% (SD {+-} 11.2%), respectively, whereas muscle and fatty tissue, tendons, bone marrow, and joint effusion revealed only a minor increase in SI. The gradient of SI (ratio SI/time) of pannus was 39.6%/min (SD {+-} 7.7%/min) and differed significantly (P < 0.001) from that of bone marrow, fatty tissue, muscle tissue, tendons, and joint effusion (P < 0.05). In contrast to synovial proliferations in rheumatoid arthritis, no differentiation between various pannus vascularities based on the degree of enhancement was possible. The Gd-DTPA-enhanced MRI studies delineate and quantify the synovial proliferations in hemophilic arthropathy. Dynamic studies in hemophilic arthropathy do not provide qualitative assessment of the inflammatory process. (orig.)

  11. Derivation of GFDM Based on OFDM Principles

    Energy Technology Data Exchange (ETDEWEB)

    Hussein Moradi; Behrouz Farhang-Boroujeny

    2015-06-01

    This paper starts with discussing the principle based on which the celebrated orthogonal frequency division multiplexing (OFDM) signals are constructed. It then extends the same principle to construct the newly introduced generalized frequency division multiplexing (GFDM) signals. This novel derivation sheds light on some interesting properties of GFDM. In particular, our derivation seamlessly leads to an implementation of GFDM transmitter which has significantly lower complexity than what has been reported so far. Our derivation also facilitates a trivial understanding of how GFDM (similar to OFDM) can be applied in MIMO channels.

  12. Deriving Framework Usages Based on Behavioral Models

    Science.gov (United States)

    Zenmyo, Teruyoshi; Kobayashi, Takashi; Saeki, Motoshi

    One of the critical issue in framework-based software development is a huge introduction cost caused by technical gap between developers and users of frameworks. This paper proposes a technique for deriving framework usages to implement a given requirements specification. By using the derived usages, the users can use the frameworks without understanding the framework in detail. Requirements specifications which describe definite behavioral requirements cannot be related to frameworks in as-is since the frameworks do not have definite control structure so that the users can customize them to suit given requirements specifications. To cope with this issue, a new technique based on satisfiability problems (SAT) is employed to derive the control structures of the framework model. In the proposed technique, requirements specifications and frameworks are modeled based on Labeled Transition Systems (LTSs) with branch conditions represented by predicates. Truth assignments of the branch conditions in the framework models are not given initially for representing the customizable control structure. The derivation of truth assignments of the branch conditions is regarded as the SAT by assuming relations between termination states of the requirements specification model and ones of the framework model. This derivation technique is incorporated into a technique we have proposed previously for relating actions of requirements specifications to ones of frameworks. Furthermore, this paper discuss a case study of typical use cases in e-commerce systems.

  13. Comparative microvascular exchange kinetics of [{sup 77}Br]bromide and {sup 99m}Tc-DTPA in humans

    Energy Technology Data Exchange (ETDEWEB)

    Cousins, Claire [Department of Clinical Radiology, Addenbrooke' s Hospital, Cambridge (United Kingdom); Skehan, Stephen J.; Rolph, Susan M.; Flaxman, Mary E.; Ballinger, James R.; Bird, Nicholas J.; Barber, Robert W.; Peters, Michael A. [Department of Nuclear Medicine, Addenbrooke' s Hospital, Cambridge (United Kingdom)

    2002-05-01

    The plasma clearance curves of small hydrophilic solutes comprise three exponentials, consistent with a three-compartmental distribution model. A previous comparison between inulin and diethylene triamine penta-acetic acid (DTPA) suggested that these three compartments are in series, the first being plasma and the second and third representing compartments within the extravascular space. Moreover, whilst the total distribution volumes of these two indicators were similar, the volume of the second compartment was higher for DTPA. The purpose of the current study was to investigate whether a solute smaller than DTPA, namely bromide, fits the hypothesis that the second space volume is an inverse function of the size of the solute. Two groups of subjects were studied: group A comprised eight patients undergoing routine diagnostic arteriography and group B, eight patients referred for routine measurement of glomerular filtration rate plus two normal volunteers. {sup 99m}Tc-DTPA and sodium [{sup 77}Br]bromide were intravenously administered simultaneously. In group A, frequent arterial samples were obtained up to 40 min after injection, and antecubital venous samples 30 s after each arterial sample. In group B, frequent venous samples were obtained up to 280 min after injection. Volume measurements based on bromide were corrected for erythrocyte bromide accumulation. In both subject groups, the normalised venous concentration ratio of bromide to DTPA, corrected for red cell bromide uptake, was significantly less than unity in the earliest blood samples, being 0.56 (SD 0.08) at 1 min, consistent with faster diffusion of bromide from plasma to interstitial fluid. Furthermore, the extraction fraction of bromide from plasma to interstitial fluid in the forearm was about 0.6, higher than that of DTPA (about 0.5) in spite of red cell bromide accumulation which equilibrated with plasma bromide within 20 s and resulted in a red cell to plasma concentration ratio of 0.51 (0

  14. New proposal for the staging of nonalcoholic steatohepatitis: Evaluation of liver fibrosis on Gd-EOB-DTPA-enhanced MRI

    Energy Technology Data Exchange (ETDEWEB)

    Tsuda, Natsuko [Medical Affairs Diagnostic Imaging, Medical Affairs, Bayer Yakuhin, Ltd. 2-4-9, Umeda, Kita-ku, Osaka 530-0001 (Japan)], E-mail: natsuko.tsuda@bayerhealthcare.com; Okada, Masahiro; Murakami, Takamichi [Department of Radiology, Kinki University School of Medicine, Osakasayama, Osaka (Japan)

    2010-01-15

    Purpose: We investigated whether the gadolinium-ethoxybenzyl-diethylenetriamine pentaacetic acid (Gd-EOB-DTPA)-enhanced MRI was useful for nonalcoholic steatohepatitis (NASH) staging based on the severity of liver fibrosis. Materials and methods: Twenty-one male Sprague-Dawley rats aged 7 weeks, weighing about 150 g in NASH group were fed a choline-deficient diet for 4, 7 or 10 weeks, and seven rats in the control group were fed a standard diet (n = 7). After the feeding period, the rats were subjected to contrast-enhanced MRI (2D-FLASH; TR/TE = 101/2.9 ms, flip angle 90 deg.). Gd-DTPA (0.1 mmol Gd/kg) and Gd-EOB-DTPA (0.025 mmol Gd/kg) were injected at 24-h intervals, and the speed of contrast injection was 1 mL/s. Signal intensities of the liver were measured and the relative enhancement (RE), the time of maximum RE (T{sub max}) and elimination half-life of RE (T{sub 1/2}) in the liver were compared. The fibrosis rate (%) was calculated with the following formula: fibrosis/whole area x 100. Results: The fibrosis rates of each group were as follows: 0.52, 0.79, 2.84, and 0.50% (4, 7, 10 weeks and control groups). The fibrosis rate of the 10 weeks group was significantly higher than the control and 4 or 7 weeks groups. Although there was no difference between the T{sub max} and T{sub 1/2} of each group after Gd-DTPA injection, the T{sub max} and T{sub 1/2} of the 10 weeks group were significantly prolonged in comparison with the control and 4 or 7 weeks groups after Gd-EOB-DTPA injection (p < 0.01). There was a significant correlation between the fibrosis rate and T{sub max} or T{sub 1/2} after Gd-EOB-DTPA injection (r = 0.90 or 0.97). Conclusion: It was possible to assess the progress of liver fibrosis in NASH by evaluating the signal intensity-time course on Gd-EOB-DTPA-enhanced MRI.

  15. Quantification of gadolinium-DTPA concentrations for different inversion times using an IR-turbo flash pulse sequence: a study on optimizing multislice perfusion imaging

    DEFF Research Database (Denmark)

    Fritz-Hansen, T; Rostrup, Egill; Ring, P B;

    1998-01-01

    a system responding linearly to input. R1 are linearly related to changes in the concentration of gadolinium (Gd)-diethylenetriaminepentaacetic acid (DTPA), and R1 is a parameter that can be derived from the magnetic resonance (MR) signal. The accuracy of calculated R1 using an IR turbo fast low-angle shot...

  16. Ion-pairing HPLC methods to determine EDTA and DTPA in small molecule and biological pharmaceutical formulations$

    Institute of Scientific and Technical Information of China (English)

    George Wang; Frank P. Tomasella

    2016-01-01

    Ion-pairing high-performance liquid chromatography–ultraviolet (HPLC–UV) methods were developed to determine two commonly used chelating agents, ethylenediaminetetraacetic acid (EDTA) in Abilifys (a small molecule drug with aripiprazole as the active pharmaceutical ingredient) oral solution and die-thylenetriaminepentaacetic acid (DTPA) in Yervoys (a monoclonal antibody drug with ipilimumab as the active pharmaceutical ingredient) intravenous formulation. Since the analytes, EDTA and DTPA, do not contain chromophores, transition metal ions (Cu2 þ , Fe3 þ ) which generate highly stable metallocom-plexes with the chelating agents were added into the sample preparation to enhance UV detection. The use of metallocomplexes with ion-pairing chromatography provides the ability to achieve the desired sensitivity and selectivity in the development of the method. Specifically, the sample preparation in-volving metallocomplex formation allowed sensitive UV detection. Copper was utilized for the de-termination of EDTA and iron was utilized for the determination of DTPA. In the case of EDTA, a gradient mobile phase separated the components of the formulation from the analyte. In the method for DTPA, the active drug substance, ipilimumab, was eluted in the void. In addition, the optimization of the concentration of the ion-pairing reagent was discussed as a means of enhancing the retention of the aminopolycarboxylic acids (APCAs) including EDTA and DTPA and the specificity of the method. The analytical method development was designed based on the chromatographic properties of the analytes, the nature of the sample matrix and the intended purpose of the method. Validation data were presented for the two methods. Finally, both methods were successfully utilized in determining the fate of the chelates.

  17. Real-time tracking of dissociation of hyperpolarized 89Y-DTPA: a model for degradation of open-chain Gd3+ MRI contrast agents

    Science.gov (United States)

    Ferguson, Sarah; Niedbalski, Peter; Parish, Christopher; Kiswandhi, Andhika; Kovacs, Zoltan; Lumata, Lloyd

    Gadolinium (Gd) complexes are widely used relaxation-based clinical contrast agents in magnetic resonance imaging (MRI). Gd-based MRI contrast agents with open-chain ligand such as Gd-DTPA, commercially known as magnevist, are less stable compared to Gd complexes with macrocyclic ligands such as GdDOTA (Dotarem). The dissociation of Gd-DPTA into Gd ion and DTPA ligand under certain biological conditions such as high zinc levels can potentially cause kidney damage. Since Gd is paramagnetic, direct NMR detection of the Gd-DTPA dissociation is quite challenging due to ultra-short relaxation times. In this work, we have investigated Y-DTPA as a model for Gd-DPTA dissociation under high zinc content solutions. Using dissolution dynamic nuclear polarization (DNP), the 89Y NMR signal is amplified by several thousand-fold. Due to the the relatively long T1 relaxation time of 89Y which translates to hyperpolarization lifetime of several minutes, the dissociation of Y-DTPA can be tracked in real-time by hyperpolarized 89Y NMR spectroscopy. Dissociation kinetic rates and implications on the degradation of open-chain Gd3+ MRI contrast agents will be discussed. This work was supported by the U.S. Department of Defense Award Number W81XWH-14-1-0048 and by the Robert A. Welch Foundation research Grant Number AT-1877.

  18. Structural determination of Zn and Cd-DTPA complexes: MS, infrared, (13)C NMR and theoretical investigation.

    Science.gov (United States)

    Silva, Vanézia L; Carvalho, Ruy; Freitas, Matheus P; Tormena, Cláudio F; Melo, Walclée C

    2007-12-31

    The joint application of MS, infrared and (13)C NMR techniques for the determination of metal-DTPA structures (metal=Zn and Cd; DTPA=diethylenetriaminepentacetic acid) is reported. Mass spectrometry allowed determining the 1:1 stoichiometry of the complexes, while infrared analysis suggested that both nitrogen and carboxyl groups are sites for complexation. The (13)C NMR spectrum for the cadmium-containing complex evidenced the existence of free and complexed carboxyl groups, due to a straight singlet at 179.0 ppm (free carboxylic (13)C) and to two broad singlets or a broad doublet at 178.3 ppm (complexed carboxylic (13)C, (2)J(Cd-C(=O))=45.2 Hz). A similar interpretation might be given for the zinc derivative and, with the aid of DFT calculations, structures for both complexes were then proposed.

  19. Toxicological study on the safety of DTPA as a drug, (1). Teratological study in the rat

    Energy Technology Data Exchange (ETDEWEB)

    Fukuda, Satoshi; Iida, Haruzo (National Inst. of Radiological Sciences, Chiba (Japan))

    1983-03-01

    In order to clarify the safety of Ca-DTPA and Zn-DTPA recommended to use as drugs in the therapeutic removal of incorporated radionuclides from the human body, the teratological study on these two agents was carried out in rats as one of a series of the toxicological tests. The teratological effects of DTPA were observed because the fetus is highly susceptible to any drug. The pregnant females of Wistar rat were injected subcutaneously daily on days 9-13 of gestation with 1, 6, 12, 24 and 36 H.D. (H.D. = human dose, 1 H.D. = 30..mu..mol/kg body weight) of Ca-DTPA or Zn-DTPA, respectively. In the dams, no toxic effects were observed. In the fetuses, the decrease of the survival rate was observed in only the group injected daily with 36 H.D. of Ca-DTPA. Some cases of gross defects of fetuses: the exencephaly, microphthalmia, anophthalmia and fusion of ribs were observed in the groups injected daily with 12, 24 and 36 H.D. of Ca-DTPA. The results obtained show that Ca-DTPA should not be given to a pregnant woman. However, no toxic effects of either Ca-DTPA or Zn-DTPA observed in the dams or of Zn-DTPA even in the fetuses indicate that these agents can be used by a radiation worker who usually is an adult man.

  20. Gadoxate disodium: gadolinium EOB DTPA, gadoxetic acid, Gd-EOB-DTPA.

    Science.gov (United States)

    2004-01-01

    Gadoxate disodium [gadolinium EOB DTPA, Gd-EOB-DTPA, gadoxetic acid, Eovist injection, Primovist] is a hydrophilic paramagnetic contrast agent being developed by Schering AG for hepatobiliary magnetic resonance imaging. In April 2004, gadoxate disodium (Primovist) was approved in Sweden, the reference member state for the EU registration. Following the Swedish approval, Schering will initiate a mutual recognition procedure for the EU with approvals expected in most countries during 2004. Gadoxate disodium is in phase III clinical trials in the US and has completed phase III studies in Japan. Submissions for approval in Japan and other Asian countries are planned for 2004. Schering AG plans to launch Eovist in Japan in 2005. Schering AG acquired a worldwide, royalty-bearing licence to EPIX Medical's patents covering liver-enhancing agents such as Eovist injection. These included a European patent (222886) and the US patents (4,899,755 and 4,888,008) that EPIX Medical exclusively licensed from the Massachusetts General Hospital (MGH). The MGH patents, a part of EPIX's extensive intellectual property, also covered albumin-targeted agents such as MS 325 (AngioMARK). Schering AG formally withdrew from the opposition proceedings against EPIX's EU patent 222886 following its acquisition of EPIX's intellectual property. These EU and US patents were also non-exclusively licensed by EPIX Medical to Bracco in September 2001. Apart from covering Eovist (Schering AG), the EU patent also covered gadobenate dimeglumine (MultiHance Bracco). Following the licensing agreement, Bracco withdrew its opposition to the patents in Europe and Japan, and both EPIX Medical and Bracco settled their European patent dispute. In its 2002 Annual Report, Schering predicted that Eovist has the potential to reach peak sales of euro50 million, three years after launch--at the time, launch in Europe was anticipated in 2004, followed by launch in Japan in 2005. This is down from earlier predictions

  1. Improved measurement of the glomerular filtration rate from Tc-99m DTPA scintigraphy in patients following nephrectomy

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Yong-il; Ha, Seunggyun [Seoul National University College of Medicine, Department of Nuclear Medicine, Seoul National University Bundang Hospital, Seongnam (Korea, Republic of); Seoul National University Bundang Hospital, Department of Nuclear Medicine, Seongnam-si, Gyeonggi-do (Korea, Republic of); So, Young [Konkuk University School of Medicine, Department of Nuclear Medicine, Chungju (Korea, Republic of); Lee, Won Woo [Seoul National University College of Medicine, Department of Nuclear Medicine, Seoul National University Bundang Hospital, Seongnam (Korea, Republic of); Seoul National University, Institute of Radiation Medicine, Medical Research Center, Seoul (Korea, Republic of); Seoul National University Bundang Hospital, Department of Nuclear Medicine, Seongnam-si, Gyeonggi-do (Korea, Republic of); Byun, Seok-Soo [Seoul National University College of Medicine, Department of Urology, Seoul National University Bundang Hospital, Seongnam (Korea, Republic of); Kim, Sang Eun [Seoul National University College of Medicine, Department of Nuclear Medicine, Seoul National University Bundang Hospital, Seongnam (Korea, Republic of); Seoul National University, Institute of Radiation Medicine, Medical Research Center, Seoul (Korea, Republic of)

    2014-02-15

    We aimed to improve Tc-99m DTPA glomerular filtration rate (GFR) scintigraphy (Gates' method) in a prospective study using Cr-51 EDTA GFR test as a gold standard. Fifty-seven Tc-99m DTPA GFR scintigrams in 45 subjects (male/female = 33:12, age = 45.9 ± 17.6 years, 14 healthy volunteers and 31 nephrectomised patients) were compared using Cr-51 EDTA GFR tests. Using the %renal uptake of Tc-99m DTPA and Cr-51 EDTA GFR, a revised equation for GFR was established through linear regression analysis. The revised equation for improved GFR was GFR(mL/min) = (%renal uptake x 11.7773) - 0.7354. Gates' original GFRs (70.1 ± 20.5 mL/min/1.73 m{sup 2}) were significantly lower than Cr-51 EDTA GFRs (97.0 ± 31.9 mL/min/1.73 m{sup 2}; P < 0.0001), but the improved GFRs (98.0 ± 26.3 mL/min/1.73 m{sup 2}) were not different from (P = 0.7360) and had a significant correlation with (r = 0.73, P < 0.0001) the Cr-51 EDTA GFRs. The revised GFR equation effectively demonstrated perioperative GFR changes in kidneys that were operated on and the contralateral kidneys at 3 and 6 months post-partial nephrectomy (n = 25). GFR measurement using Tc-99m DTPA scintigraphy could be significantly improved by a revised equation derived from the comparison with Cr-51 EDTA GFR. (orig.)

  2. In vivo metabolite compartmentalization probed using intracellular GdDTPA

    DEFF Research Database (Denmark)

    Peters, David Alberg; Rowland, Ian

    Fast trans-membrane water exchange enables in- tracellular relaxation enhancement of water by contrast agents in the extracellular space. For me- tabolites not in fast exchange across membranes, intracellular metabolite relaxation enhancement will only occur if the contrast agent and metabolite a...... are in the same compartment. Extracellular contrast has utilized electroporation methods to deliver GdDTPA into the cytosol of rat muscle in vivo in order to probe the intracellular compart- mentalization of MR-visible metabolites....

  3. Comparative biodistribution of potential anti-glioblastoma conjugates [111In]DTPA-hEGF and [111In]Bz-DTPA-hEGF in normal mice.

    Science.gov (United States)

    Tolmachev, Vladimir; Orlova, Anna; Wei, Qichun; Bruskin, Alexander; Carlsson, Jörgen; Gedda, Lars

    2004-08-01

    EGF-receptors (EGFR) are overexpressed in gliomas, as well as in tumors of breast, lung, and urinary bladder. For this reason, EGFR may be an attractive target for both visualization and therapy of malignant tumors using radioactive nuclides. Natural ligand of EGFR, epidermal growth factor (EGF) is a small 53-amino-acid protein. Low molecular weight of EGF may enable better intratumoral penetration in comparison to antibodies. [111In]DTPA-EGF was proposed for the targeting of glioblastoma and breast cancer, and its tumor-seeking properties were confirmed in animal studies. The aim of this study was to evaluate how the substitution of heptadentate DTPA for octadentate benzyl-DTPA (Bz-DTPA) effects the biodistribution of indium-labeled human EGF (hEGF) in normal NMRI mice. [111In]DTPA-hEGF and [111In]Bz-DTPA-hEGF, obtained by the coupling of ITC-benzyl-DTPA to hEGF, were injected into the tail vein. At 0.5, 1, 4, and 24 hours postinjection, the animals were sacrificed, and radioactivity in different organs was measured. The blood clearance of both conjugates was fast. The uptake of both conjugates in the liver, spleen, stomach, pancreas, intestines, and submaxillary gland was most likely receptor-mediated. The uptake in a majority of organs was similar. However, indium uptake in the case of [111In]DTPA-hEGF was significantly higher in the kidneys and bones. In conclusion, [111In]Bz-DTPA-hEGF seems to have more favourable in vivo distribution in comparison to [111In]DTPA-hEGF.

  4. Spectroscopic studies on interaction of BSA and Eu(III) complexes with H5ph-dtpa and H5dtpa ligands.

    Science.gov (United States)

    Kong, Deyong; Qin, Cui; Fan, Ping; Li, Bing; Wang, Jun

    2015-04-01

    An novel aromatic aminopolycarboxylic acid ligand, N-(2-N,N-Dicarboxymethylaminophenyl) ethylenediamine-N,N',N'-triacetic acid (H5ph-dtpa), was synthesized by improving experimental method and its corresponding Eu(III) complex, Na2[EuIII(ph-dtpa)(H2O)]·6H2O, was successfully prepared through heat-refluxing method. As a comparison, the Eu(III) complex with diethylenetriamine-N,N,N',N',N″-pentaacetic acid (H5dtpa) ligand, Na2[Eu(III)(dtpa)(H2O)]·6H2O, was also prepared by the same method. And then, the interaction between prepared Eu(III) complexes ([EuIII(dtpa)(H2O)]2- and [EuIII(ph-dtpa)(H2O)]2-) and bovine serum albumin (BSA) in aqueous solution were studied by the combination of ultraviolet-visible (UV-vis), fluorescence and circular dichroism (CD) spectroscopies. In addition, the binding sites of Eu(III) complexes ([EuIII(dtpa)(H2O)]2- and [EuIII(ph-dtpa)(H2O)]2-) to BSA molecules were also estimated by synchronous fluorescence. Moreover, the theoretical and experimental results show that the Van der Waals, hydrogen bond and π-π stacking interactions are the mainly impulse to the reaction. The binding distances (r) between Eu(III) complexes ([EuIII(dtpa)(H2O)]2- and [EuIII(ph-dtpa)(H2O)]2-) and BSA were obtained according to Förster's non-radiative energy transfer theory. Also, the determined UV-vis absorption spectroscopy, synchronous fluorescence and circular dichroism (CD) spectra showed that the conformation of BSA could be changed in the presence of Eu(III) complexes. The obtained results can help understand the action mode between rare earth metal complexes of aminopolycarboxylic acid ligands with BSA and they are also expected to provide important information of designs of new inspired drugs.

  5. Analysis of pharmacokinetics of Gd-DTPA for dynamic contrast-enhanced magnetic resonance imaging.

    Science.gov (United States)

    Taheri, Saeid; Shah, N Jon; Rosenberg, Gary A

    2016-09-01

    The pharmacokinetics (PK) of the contrast agent Gd-DTPA administered intravenously (i.v.) for contrast-enhanced MR imaging (DCE-MRI) is an important factor for quantitative data acquisition. We studied the effect of various initial bolus doses on the PK of Gd-DTPA and analyzed population PK of a lower dose for intra-subject variations in DCE-MRI. First, fifteen subjects (23-85years, M/F) were randomly divided into four groups for DCE-MRI with different Gd-DTPA dose: group-I, 0.1mmol/kg, n=4; group-II, 0.05mmol/kg, n=4; group-III, 0.025mmol/kg, n=4; and group-IV, 0.0125mmol/kg, n=3. Sequential fast T1 mapping sequence, after a bolus i.v. Gd-DTPA administered, and a linear T1-[Gd-DTPA] relationship were used to estimate the PK of Gd-DTPA. Secondly, MR-acquired PKs of Gd-DTPA from 58 subjects (28-80years, M/F) were collected retrospectively, from an ongoing study of the brain using DCE-MRI with Gd-DTPA at 0.025mmol/kg, to statistically analyze population PK of Gd-DTPA. We found that the PK of Gd-DTPA (i.v. 0.025mmol/kg) had a half-life of 37.3±6.6min, and was a better fit into a linear T1-[Gd-DTPA] relationship than higher doses (up to 0.1mmol/kg). The area under the curve (AUC) for 0.025mmol/kg was 3.37±0.46, which was a quarter of AUC of 0.1mmol/kg. In population analysis, a dose of 0.025mmol/kg of Gd-DTPA provided less than 5% subject-dependent variation in the PK of Gd-DTPA. Administration of 0.025mmol/kg Gd-DTPA enabled us to estimate [Gd-DTPA] from T1 by using a linear relationship that has a lower estimation error compared to a non-linear relationship. DCE-MRI with a quarter dose of Gd-DTPA is more sensitive to detect changes in [Gd-DTPA].

  6. Actinides exposure: review of Ca-DTPA injections inside Cea-Cogema plants; Exposition aux actinides: bilan des injections de Ca-DTPA dans les centres CEA-Cogema

    Energy Technology Data Exchange (ETDEWEB)

    Grappin, L.; Berard, P.; Beau, P.; Carbone, L.; Castagnet, X.; Courtay, C.; Le Goff, J.P.; Menetrier, F.; Neron, M.; Piechowski, J. [CEA Cadarache, Dir. de l' Energie Nucleaire, Dept. de Soutien en surete et securite, Sev. de Sante au Travail, 13 - Saint-Paul-lez-Durance (France)

    2006-07-01

    Ca-DTPA has been used for medical treatment of plutonium and americium contaminations in the CEA and COGEMA plants from 1970 to 2003. This report is a survey of the injections administered of Ca-DTPA as a chelating molecule. This report will be a part of the AMM process for Ca-DTPA by intravenous administration submitted by the Central Pharmacy of the french Army. Out of 1158 injections administered to 469 persons, 548 events of possible or confirmed contaminations were reported. These employees were followed by occupational physicians according to the current regulations. The first part of the report is a synthesis of the most recent findings. Due to its short biological period and its limited action in the blood, Ca-DTPA does not chelate with plutonium and americium as soon as these elements are deposited in the target organs. It justifies an early treatment, even in cases of suspected contamination followed by additional injections if necessary. The second part presents data concerning these 1158 injections (way of contamination, posology, adverse effects...). These incidents took place at work, were most often minor, not requiring follow-up treatment. A study concerning the effectiveness of the product was done on a group of people having received 5 or more injections. These results were compared with effectiveness estimated from theoretical basis. Posologies and therapeutic schemes were proposed based on these observations. Additional studies are needed to confirm these findings. This document is the first synthesis in this field. It is the result of a collective work having mobilized the occupational medicine departments, the laboratories of CEA and COGEMA and a working group CEA-COGEMA-SPRA. (authors)

  7. Potential Opponent for 18FDG: Gd3+-DTPA-DG: A New Synthetic MRI Contrast Agent

    Directory of Open Access Journals (Sweden)

    Mehdi Shafiee Ardestani

    2010-05-01

    Full Text Available Glucose mediated imaging tools have recently be-come an area of research interest in the field of nuclear medicine, especially Positron Emission Tomography (PET imaging. The difficulties in the use, preparation and cost of radioactively-labeled glycosylated compounds led to the research and development in this present study of a new gadolinium-labeled glucose that does not have a radioactive half-life or difficulties in its synthesis and utilization. Based on the structure of fluorodeoxyglucose (18FDG, a new compound consisting of a D-glucose conjugated to a well-known chelator DTPA was synthesized, labeled with Gd3+ and examined in vitro and in vivo. The results showed a good anti-cancer potency for the prepared complex. Gd3+ -DTPA-DG did not produce any significant alteration in the blood glucose level and was also phosphorylated by hexokinase enzyme. In vitro relaxation times T1 and T2 were determined and compared with a standard compound Magnevist®. Finally, imaging studies were performed in tumor-bearing mice and the tumor areas were shown successfully. This compound may have great potential in the future in radio-oncology.

  8. Dynamic MR imaging of kidneys perfused with EOB-Gd-DTPA.

    Science.gov (United States)

    Hyodoh, Kazusa; Hyodoh, Hideki; Kasahara, Michiko; Washio, Yoshikazu; Asai, Mayumi; Hareyama, Masato

    2011-01-01

    Gandolinium-ethoxybenzyl-diethylenetriamine pentaacetic acid (Gd-EOB-DTPA) is a hepatobiliary contrast agent for MRI. It was reported that Gd-EOB-DTPA is useful to detect liver tumors and differentially diagnose benign and malignant pathologies in the liver. Since Gd-EOB-DTPA partially accumulates in the hepatocytes and bile via various transporters after intravenous injection, signal intensity in the liver increases on T1-weighted images. The signal intensity of the liver after Gd-EOB-DTPA injection depends on the Gd-EOB-DTPA uptake by hepatocytes and bile excretion. It is known tha the Gd-EOB-DTPA accumulating in the kidney is excreted to the urine through glomerular filtration. Because Gd-DTPA is concentrated in the renal tubules after being filtered at the Bowman's capsule, and since it is neither secreted nor reabsorbed the concentrating and diluting function of the renal tubules can be studied by imaging techniques. since renal function can be evaluated with Gd-EOB-DTPA can also be used to evaluate renal function. Eith the development of MRI equipment and rapid imaging techniques, temporal resolution had improved greatly. However, no previous study has been carried out on renal function using Gd-EOB-DTPA dynamic MR study that was correlated with estimated glomerular filtration rate (eGFR) and stage of chronic kidney disease (CKD) in the Japan Association of chronic kidney disease initiative.

  9. The use of EDTA and DTPA for accelerating the removal of deposited transuranic elements from humans

    CERN Document Server

    Spoor, N L

    1977-01-01

    EDTA and DTPA have been prominent among the chelating agents used to increase the rate of excretion of certain deposited heavy metals from the human body. Since 1959, DTPA, administered either by intravenous injection or by aerosol inhalation, has been widely used to treat workers contaminated by plutonium or a higher actinide. In this report, an attempt is made to assess the toxicities of EDTA and DTPA and to evaluate the effectiveness and safety of DTPA as a drug for removing deposited transuranic elements.

  10. Assessing liver function by liver enhancement during the hepatobiliary phase with Gd-EOB-DTPA-enhanced MRI at 3 Tesla

    Energy Technology Data Exchange (ETDEWEB)

    Verloh, N.; Haimerl, M.; Schlabeck, M.; Schreyer, A.G.; Stroszczynski, C.; Fellner, C.; Wiggermann, P. [University Hospital Regensburg, Department of Radiology, Regensburg (Germany); Zeman, F. [University Hospital Regensburg, Center for Clinical Trials, Regensburg (Germany); Barreiros, A. [University Hospital Regensburg, Department of Gastroenterology, Regensburg (Germany); Loss, M. [University Hospital Regensburg, Department of Surgery, Regensburg (Germany)

    2014-05-15

    The purpose of this study was to evaluate the usefulness of Gd-EOB-DTPA-enhanced 3-T MRI to determine the hepatic functional reserve expressed by the model for end-stage liver disease (MELD) score. A total of 121 patients with normal liver function (NLF; MELD score ≤ 10) and 29 patients with impaired liver function (ILF; MELD score > 10) underwent contrast-enhanced MRI with a hepatocyte-specific contrast agent at 3T. T1-weighted volume interpolated breath-hold examination (VIBE) sequences with fat suppression were acquired before and 20 min after contrast injection. Relative enhancement (RE) between plain signal intensity and contrast-enhanced signal intensity was calculated and was used to determine Gd-EOB-DTPA uptake into the liver parenchyma for patients with different MELD scores. RE differed significantly (p ≤ 0.001) between patients with NLF (87.2 ± 29.5 %) and patients with ILF (45.4 ± 26.5 %). The optimal cut-off value for RE to differentiate NLF from ILF was 47.7 % (AUC 0.87). This cut-off value showed a sensitivity of 82.8 % and a specificity of 92.7 % for the differentiation of the analysed groups. Gd-EOB-DTPA uptake in hepatocytes is strongly affected by liver function. Gd-EOB-DTPA-enhanced MRI and assessment of RE during the hepatobiliary phase (HBP) may serve as a useful image-based test in liver imaging for determining regional and global liver function. (orig.)

  11. MR Prediction of Liver Function and Pathology Using Gd-EOB-DTPA: Effect of Liver Volume Consideration

    Directory of Open Access Journals (Sweden)

    Dai Shimamoto

    2015-01-01

    Full Text Available Purpose. To evaluate whether the diagnostic performance of Gd-EOB-DTPA-enhanced MRI in evaluating liver function and pathology is improved by considering liver volume (LV. Methods. This retrospective study included 104 patients who underwent Gd-EOB-DTPA-enhanced MRI before liver surgery. For each patient, using the precontrast and hepatobiliary phase images, we calculated the increase rate of the liver-to-spleen signal intensity ratio (LSR, that is, the “ΔLSR,” and the increase rate of the liver-to-muscle signal intensity ratio (LMR, that is, the “ΔLMR.” ΔLSR × LV and ΔLMR × LV were also calculated. The correlation of each MR parameter with liver function data or liver pathology was assessed. The correlation coefficients were compared between ΔLSR (ΔLMR and ΔLSR (ΔLMR × LV. Results. The correlation coefficient between ΔLSR (ΔLMR × LV and cholinesterase was significantly higher than that between ΔLSR (ΔLMR and cholinesterase. The correlation coefficient between ΔLSR (ΔLMR × LV and the degree of fibrosis or necroinflammatory activity was significantly lower than that between ΔLSR (ΔLMR and the degree of fibrosis or necroinflammatory activity. Conclusion. The inclusion of liver volume may improve Gd-EOB-DTPA-based predictions of liver function, but not in predictions of liver pathology.

  12. Improvement in DTPA efficacy for plutonium decorporation: biokinetic and dosimetric approaches

    Energy Technology Data Exchange (ETDEWEB)

    Serandour, A.L.; Grillon, G.; Taulelle, C.; Frechou, M.; Ben Salah, M.; Fritsch, P.; Le Gall, B.; Poncy, J.L. [CEA Bruyeres-le-Chatel (DSV/DRR), Lab. Radiotoxicologie, 91 (France); Gervelas, C.; Tsapis, N.; Fatal, E. [Paris-11 Univ., UMR CNRS 8612, Physico-chimie-Pharmacotechnie-Biopharmacie, 92 - Chatenay-Malabry (France); Phan, G.; Benech, H. [CEA Saclay (DSV/DRM), Service de Pharmacologie et d' Immunologie, 91 - Gif-sur-Yvette (France); Deverre, J.R. [Service Hospitalier Frederic Joliot (CEA/DSV/DRM), 91 - Gif-sur-Yvette (France)

    2006-07-01

    After accidental contamination, the main goal of decorporation is to reduce the dose delivered to the target tissues. In the case of plutonium, the main retention organs are the liver and the skeleton. Diethylenetriamine pentaacetic acid (DTPA ) is the only molecule efficient to de-corporate plutonium which can be administrated to humans. However, its efficiency is limited due to its fast urinary excretion and its distribution, mainly limited in blood and biological fluids. Our goal is to optimise the decorporation efficiency of DTPA by increasing its half-life in blood and allowing the concordance of distribution between the chelate and the actinide. We propose two novel formulations of DTPA according to the chemical form of radionuclides and the type of contamination. First we have shown that encapsulation of DTPA into liposomes could induce major modifications of the chelate pharmacokinetic parameters. For this study, DTPA was encapsulated into conventional and stealth liposomes (large and small vesicles) [1]. Their pharmacokinetics and ability to de-corporate Pu were evaluated in rats treated by a single injection of vectorized DTPA, two hours after contamination with colloidal or soluble Pu [2]. Results showed an increase of the plasma half-life of DTPA up to 12 h and an accumulation of DTPA in liver, spleen and bone. In addition, administration of liposomes containing DTPA induced a significant decrease of Pu retention in the liver (factor 2 compared to free DTPA ) and a large decrease of Pu deposition in the bone (factor 10 compared to free DTPA). These data were associated with an urinary excretion of 80% of injected Pu over 16 days. Thus, these formulations of DTPA encapsulated into liposomes were more efficient to de-corporate Pu than free DTPA. This novel de-corporating strategy could be efficiently used in contaminated patients to avoid long term retention of Pu in the body. Another approach consists in the development of a dry powder formulation of

  13. Incidental 99mTc-DTPA Uptake in Tarlov Cysts on Radionuclide SPECT/CT Cisternography.

    Science.gov (United States)

    Vamadevan, Shankar; Le, Ken; Bui, Chuong; Mansberg, Robert

    2017-04-01

    Sacral perineural cysts are also known as Tarlov cysts. A 58-year-old man with suspected intracranial hypotension was evaluated with Tc-DTPA radionuclide cisternography. Radionuclide planar and SPECT/CT cisternography revealed Tc-DTPA uptake in sacral lesions. Spine MRI confirmed Tarlov cysts at the S1 and S2 levels.

  14. Synthesis of Pharmacological Heterocyclic Derivatives Based Surfactants.

    Science.gov (United States)

    El-Sayed, Refat; Fadda, Ahmed A

    2016-01-01

    Synthesis of chromenopyrimidine derivatives and the related fused system carried out by the reaction of chromene derivative 1 with various reagents under suitable reaction conditions. Condensation of stearoyl chloride with these heterocycles, then, propoxylated the products using propylene oxide to produce surface active agents having a twofold capacity as surface and antimicrobial dynamic specialists which may be served in the production of medications, pesticides, beautifying agents or may be utilized as an antimicrobial. Some of the surface properties and antimicrobial activity were resolved.

  15. Respiratory clearance of 99mTc-DTPA and pulmonary involvement in sarcoidosis

    Energy Technology Data Exchange (ETDEWEB)

    Dusser, D.J.; Collignon, M.A.; Stanislas-Leguern, G.; Barritault, L.G.; Chretien, J.; Huchon, G.J.

    1986-09-01

    To investigate the relationships between the respiratory epithelial clearance of micronic aerosolized /sup 99m/Tc-DTPA (RC-DTPA) and pulmonary function, serum angiotensin-converting enzyme (SACE), and lymphocytic alveolitis in patients with sarcoidosis, RC-DTPA was measured in 49 nonsmokers with pulmonary sarcoidosis and 38 normal nonsmokers. Pulmonary involvement was evaluated on chest roentgenograms (type O = normal, type I = hilar adenopathies, type II = hilar adenopathies associated with parenchymal shadows, type III = parenchymal shadows without adenopathy) and by pulmonary function tests. Serum angiotensin-converting enzyme was determined, and a bronchoalveolar lavage was performed for alveolar lymphocyte differential counting (Ly%). RC-DTPA was increased (greater than or equal to 1.96%/min) in 12 of 31 patients with type II or III involvement but was normal in all 18 patients with type O or I involvement (p = 0.002). Patients with increased RC-DTPA had low FVC, TLC, FEV1, and resting Pao2 (p less than 0.05); resting and exercise AaPo2 were increased (p less than 0.05), but RC-DTPA correlated negatively with FEV1 (p less than 0.01), Pao2 at rest (p less than 0.005), and DLCO (p less than 0.05) and positively with resting and exercise AaPO2 (p less than 0.01). In patients with increased RC-DTPA (42 +/- 17%), Ly% did not differ from Ly% in patients with normal RC-DTPA (34 +/- 16%). SACE was increased in patients with increased RC-DTPA (56 +/- 26 U/ml versus 38 +/- 16 U/ml; p = 0.007) and correlated positively with RC-DTPA (p less than 0.001).(ABSTRACT TRUNCATED AT 250 WORDS)

  16. Pricing Model of Multiattribute Derivatives Based on Mixed Process

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    By Analyzing the behavior and character of derivative security, the authorsestablished a pricing model of multiattribute derivative security whose underlying asset pricingprocess is a mixed process, and obtained a new model for option pricing of multiattribute derivatives based on mixed process, and improved some original results.

  17. A derivability criterion based on the existence of adjunctions

    CERN Document Server

    Rodriguez-Gonzalez, Beatriz

    2012-01-01

    In this paper we introduce a derivability criterion of functors based on the existence of adjunctions rather than on the existence of resolutions. It constitutes a converse of Quillen-Maltsiniotis derived adjunction theorem. We present two applications of our derivability criterion. On the one hand, we prove that the two notions for homotopy colimits corresponding to Grothendieck derivators and Quillen model categories are equivalent. On the other hand, we deduce that the internal hom for derived Morita theory constructed by B. To\\"en is indeed the right derived functor of the internal hom of dg-categories.

  18. Enhanced conjugation stability and blood circulation time of macromolecular gadolinium-DTPA contrast agent

    Energy Technology Data Exchange (ETDEWEB)

    Jenjob, Ratchapol [Department of New Drug Development, School of Medicine, Inha University, 2F A-dong, Jeongseok Bldg., Sinheung-dong 3-ga, Jung-gu, Incheon 400-712 (Korea, Republic of); Kun, Na [Department of Biotechnology, The Catholic University of Korea, 43 Jibong-ro, Wonmi-gu, Bucheon-si, Gyeonggi-do 420-743 (Korea, Republic of); Ghee, Jung Yeon [Utah-Inha DDS and Advanced Therapeutics, B-403 Meet-You-All Tower, SongdoTechnopark, 7–50, Songdo-dong, Yeonsu-gu, Incheon 406-840 (Korea, Republic of); Shen, Zheyu; Wu, Xiaoxia [Division of Functional Materials and Nano-Devices, Ningbo Institute of Materials Technology & Engineering (NIMTE), Chinese Academy of Sciences, 519 Zhuangshi Street, Zhenhai District, Ningbo, Zhejiang 315201 (China); Cho, Steve K., E-mail: scho@gist.ac.kr [Division of Liberal Arts and Science, GIST College, Gwangju Institute of Science and Technology, Gwangju 500-712 (Korea, Republic of); Lee, Don Haeng [Utah-Inha DDS and Advanced Therapeutics, B-403 Meet-You-All Tower, SongdoTechnopark, 7–50, Songdo-dong, Yeonsu-gu, Incheon 406-840 (Korea, Republic of); Department of Internal Medicine, School of Medicine, Inha University Hospital, Incheon 420-751 (Korea, Republic of); Yang, Su-Geun, E-mail: Sugeun.Yang@Inha.ac.kr [Department of New Drug Development, School of Medicine, Inha University, 2F A-dong, Jeongseok Bldg., Sinheung-dong 3-ga, Jung-gu, Incheon 400-712 (Korea, Republic of)

    2016-04-01

    In this study, we prepared macromolecular MR T1 contrast agent: pullulan-conjugated Gd diethylene triamine pentaacetate (Gd-DTPA-Pullulan) and estimated residual free Gd{sup 3+}, chelation stability in competition with metal ions, plasma and tissue pharmacokinetics, and abdominal MR contrast on rats. Residual free Gd{sup 3+} in Gd-DTPA-Pullulan was measured using colorimetric spectroscopy. The transmetalation of Gd{sup 3+} incubated with Ca{sup 2+} was performed by using a dialysis membrane (MWCO 100–500 Da) and investigated by ICP-OES. The plasma concentration profiles of Gd-DTPA-Pullulan were estimated after intravenous injection at a dose 0.1 mmol/kg of Gd. The coronal-plane abdominal images of normal rats were observed by MR imaging. The content of free Gd{sup 3+}, the toxic residual form, was less than 0.01%. Chelation stability of Gd-DTPA-Pullulan was estimated, and only 0.2% and 0.00045% of Gd{sup 3+} were released from Gd-DTPA-Pullulan after 2 h incubation with Ca{sup 2+} and Fe{sup 2+}, respectively. Gd-DTPA-Pullulan displayed the extended plasma half-life (t{sub 1/2,α} = 0.43 h, t{sub 1/2,β} = 2.32 h), much longer than 0.11 h and 0.79 h of Gd-EOB-DTPA. Abdominal MR imaging showed Gd-DTPA-Pullulan maintained initial MR contrast for 30 min. The extended plasma half-life of Gd-DTPA-Pullulan probably allows the prolonged MR acquisition time in clinic with enhanced MR contrast. - Highlights: • Macromolecule (pullulan) conjugated Gd contrast agent (Gd-DTPA-Pullulan) showed the extended plasma half-life (t{sub 1/2,α} = 0.43 h, t{sub 1/2,β} = 2.32 h) in comparison with Gd-EOB-DTPA • Gd-DTPA-pullulan T1 contrast agent exhibited strong chelation stability against Gd. • The extended blood circulation attributed the enhanced and prolonged MR contrast on abdominal region of rats. • The extended blood circulation may provide prolonged MR acquisition time window in clinics.

  19. Asymmetric Schiff bases derived from diaminomaleonitrile and their metal complexes

    Science.gov (United States)

    Yang, Jianjie; Shi, Rufei; Zhou, Pei; Qiu, Qiming; Li, Hui

    2016-02-01

    Asymmetric Schiff bases, due to its asymmetric structure, can be used as asymmetric catalyst, antibacterial, and mimic molecules during simulate biological processes, etc. In recent years, research on synthesis and properties of asymmetric Schiff bases have become an increase interest of chemists. This review summarizes asymmetric Schiff bases derived from diaminomaleonitrile (DAMN) and DAMN-based asymmetric Schiff bases metal complexes. Applications of DAMN-based asymmetric Schiff bases are also discussed in this review.

  20. Gd-DTPA-enhanced lesions in the brain of patients with acute optic neuritis

    DEFF Research Database (Denmark)

    Christiansen, P; Frederiksen, J L; Henriksen, O

    1992-01-01

    The cerebral hemispheres of 19 patients with acute monosymptomatic optic neuritis (AMON) were investigated using magnetic resonance imaging (MRI) and Gadolinium-DTPA (Gd-DTPA). Using T1-weighted and T2-weighted imaging sequences it was disclosed that 14 of 19 patients had lesions and that enhance......The cerebral hemispheres of 19 patients with acute monosymptomatic optic neuritis (AMON) were investigated using magnetic resonance imaging (MRI) and Gadolinium-DTPA (Gd-DTPA). Using T1-weighted and T2-weighted imaging sequences it was disclosed that 14 of 19 patients had lesions......, and that an enhancing silent lesion represents an acute MS lesion, we may hypothesize that the disease process ultimately leading to MS starts long before the first symptom or sign ever appears. It would seem that at least half of the silent lesions in the cerebral hemispheres of patients with AMON had existed before...

  1. Use of paramagnetic chelated metal derivatives of polysaccharides and spin-labeled polysaccharides as contrast agents in magnetic resonance imaging

    Energy Technology Data Exchange (ETDEWEB)

    Bligh, S.W.; Harding, C.T.; Sadler, P.J.; Bulman, R.A.; Bydder, G.M.; Pennock, J.M.; Kelly, J.D.; Latham, I.A.; Marriott, J.A. (Department of Chemistry, Birkbeck College, London (England))

    1991-02-01

    Soluble and insoluble polysaccharides were derivatized with diethylenetriaminepentaacetic acid (DTPA) and/or spin-labeled with 2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO). Polysaccharides derivatized with DTPA were prepared via cyanogen bromide activation, coupling to a diamine linker, and to DTPA anhydride. Spin-labeled polysaccharides were also prepared via cyanogen bromide activation. The extent of derivatization for dextran (18 kDa) was about 120 glucose units per DTPA, and for cellulose and starch about 15-30 units per DTPA. For spin-labeled polysaccharides, the average loading ranged from 1 nitroxide per 16 glucose units for starch to 181 for dextran (82 kDa). These derivatized paramagnetic polysaccharides were shown to be more effective relaxants than the small paramagnetic molecules alone. Both soluble and insoluble polysaccharide-linker-DTPA-Gd(3) complexes were effectively cleared from the body (rats) after oral administration. After intravenous administration, the biodistribution of dextran-linker-DTPA-Gd(3) complexes differed significantly from that of GdDTPA. Reduction of the nitroxide by ascorbic acid was retarded in the polysaccharide derivatives, particularly in starch derivatized with both nitroxide and linker-DTPA-Cu(2). These agents showed contrast enhancement in the gastrointestinal tract of rabbits.

  2. Evaluation of inflammatory bowel disease with /sup 99mTc-DTPA

    Energy Technology Data Exchange (ETDEWEB)

    Kadir, S.; Strauss, H.W.

    1979-02-01

    Technetium-99m-labelled DTPA was found to localize in segments of bowel with inflammation due to ulcerative colitis, regional enteritis, and other forms of enterocolitis. The concentration of tracer was apparently related to the clinical activity of the disease process. Imaging with /sup 99m/Tc-DTPA may offer an appealing, noninvasive alternative for identifying and following up patients with inflammatory bowel disease.

  3. A Facile Synthesis of Indole-based Conjugated Derivatives

    Institute of Scientific and Technical Information of China (English)

    LI, Qian-Qian; ZHU, Zhi-Chao; LI,Zhen; QIN, Jin-Gui

    2007-01-01

    A novel synthetic method was developed for the preparation of indole-based conjugated derivatives with satisfied yields. By applying this strategy, a series of new compounds were prepared conveniently. All the obtained new indole derivatives were characterized by spectroscopic analyse s, giving satisfactory data corresponding to their expected molecular structures.

  4. Multivariable analysis of clinical influence factors on liver enhancement of Gd-EOB-DTPA-enhanced 3T MRI; Multivariable Analyse klinischer Einflussfaktoren auf die Signalintensitaet bei Gd-EOB-DTPA 3T-MRT der Leber

    Energy Technology Data Exchange (ETDEWEB)

    Verloh, N.; Haimerl, M.; Stroszczynski, C.; Fellner, C.; Wiggermann, P. [University Hospital Regensburg (Germany). Dept. of Radiology; Zeman, F. [University Hospital Regensburg (Germany). Center for Clinical Trials; Teufel, A. [University Hospital Regensburg (Germany). Dept. of Gastroenterology; Lang, S. [University Hospital Regensburg (Germany). Dept. of Surgery

    2015-01-15

    The purpose of this study was to identify clinical factors influencing Gd-EOB-DTPA liver uptake in patients with healthy liver parenchyma. A total of 124 patients underwent contrast-enhanced MRI with a hepatocyte-specific contrast agent at 3T. T1-weighted volume interpolated breath-hold examination (VIBE) sequences with fat suppression were acquired before and 20 minutes after contrast injection. The relative enhancement (RE) between plain and contrast-enhanced signal intensity was calculated. Simple and multiple linear regression analyses were performed to evaluate clinical factors influencing the relative enhancement. Patients were subdivided into three groups according to their relative liver enhancement (HRE, RE ≥ 100 %; MRE, 100 % > RE > 50 %; NRE, RE ≤ 50 %) and were analyzed according to the relevant risk factors. Simple regression analyses revealed patient age, transaminases (AST, ALT, GGT), liver, spleen and delta-liver volume (the difference between the volumetrically measured liver volume and the estimated liver volume based on body weight) as significant factors influencing relative enhancement. In the multiple analysis the transaminase AST, spleen and delta liver volume remained significant factors influencing relative enhancement. Delta liver volume showed a significant difference between all analyzed groups. Liver enhancement in the hepatobiliary phase depends on a variety of factors. Body weight-adapted administration of Gd-EOB-DTPA may lead to inadequate liver enhancement after 20 minutes especially when the actual liver volume differs from the expected volume.

  5. Magnetic resonance imaging of the parotid gland. Diagnosis by plain imaging and with contrast medium Gd-DTPA. Kernspintomographie der Glandula parotis. Nativdiagnostik und Gd-DTPA

    Energy Technology Data Exchange (ETDEWEB)

    Vogl, T.; Dresel, S.; Kang, K.; Lissner, J. (Muenchen Univ. (Germany, F.R.). Radiologische Klinik und Poliklinik); Grevers, G.; Riederer, A. (Muenchen Univ. (Germany, F.R.). Klinik und Poliklinik fuer Hals-, Nasen- und Ohrenkranke); Spaeth, M. (Muenchen Univ. (Germany, F.R.). Medizinische Poliklinik)

    1989-06-01

    Pathological lesions of the parotid gland were examined comparatively with different examination sequences both plain and with the contrast medium Gd-DTPA. There were 36 benign lesions (parotitis, Sjoegren's syndrome, adenoma, etc.) and 24 malignant tumours (squamous cell carcinoma, adenocarcinoma, adenoid cystic carcinoma etc.) Examinations were carried out at 1.0 T with long and short spin echo sequences in transverse and frontal layer orientation before and after application of Gd-DTPA as contrast medium. In the patients suffering from parotitis the best results were obtained with plain T{sub 1} and T{sub 2} sequences; the contrast medium Gd-DTPA bemained without superior diagnostic relevance. However, in Sjoegren's syndrome (myoepithelial sialadenitis) administration of the contrast medium alwys yielded a characteristic honeycomblike pattern. In benign and malignant space-occupying growths MRI supplied additional diagnostic information with Gd-DTPA in respect of defining the tumour borderlines and paths of infiltration. MRI is now a significant diagnostic tool in inflammatory and tumorous lesions of the parotid gland. (orig.).

  6. Simplified structure of a new model to describe urinary excretion of plutonium after systemic, liver or pulmonary contamination of rats associated with Ca-DTPA treatments.

    Science.gov (United States)

    Fritsch, P; Sérandour, A L; Grémy, O; Phan, G; Tsapis, N; Abram, M C; Renault, D; Fattal, E; Benech, H; Deverre, J R; Poncy, J L

    2009-06-01

    This study validates, by targeted experiments, several modeling hypotheses for interpretation of urinary excretion of plutonium after Ca-DTPA treatments. Different formulations and doses of Ca-DTPA were administered to rats before or after systemic, liver or lung contamination with various chemical forms of plutonium. The biokinetics of plutonium was also characterized after i.v. injection of Pu-DTPA. Once formed, Pu-DTPA complexes are stable in most biological environments. Pu-DTPA present in circulating fluids is rapidly excreted in the urine, but 2-3% is retained, mainly in soft tissues, and is then excreted slowly in the urine after transfer to blood. Potentially, all intracellular monoatomic forms of plutonium could be decorporated after DTPA internalization involving slow urinary excretion of Pu-DTPA with half-lives varying from 2.5 to 6 days as a function of tissue retention. The ratio of fast to slow urinary excretion of Pu-DTPA depends on both plutonium contamination and Ca-DTPA treatment. Fast urinary excretion of Pu-DTPA corresponds to extracellular decorporation that occurs beyond a threshold of the free DTPA concentration in circulating fluids. Slow excretion corresponds mostly to intracellular decorporation and depends on the amount of intracellular DTPA. From these results, the structure of a simplified model is proposed for interpretation of data obtained with Ca-DTPA treatments after systemic, wound or pulmonary contamination by plutonium.

  7. Decorporation approach following rat lung contamination with a moderately soluble compound of plutonium using local and systemic Ca-DTPA combined chelation.

    Science.gov (United States)

    Grémy, Olivier; Tsapis, Nicolas; Bruel, Sylvie; Renault, Daniel; Van der Meeren, Anne

    2012-09-01

    Decorporation efficacy of prompt pulmonary delivery of DTPA dry powder was assessed following lung contamination with plutonium nitrate and compared to an intravenous injection of DTPA solution and a combined administration of both DTPA compounds. In addition, efficacy of a delayed treatment was assessed. In case of either early or late administration, insufflated DTPA was more efficient than intravenously injected DTPA in reducing the plutonium lung burden due to its high local concentration. Prompt treatment with DTPA powder was also more effective in limiting extrapulmonary deposits by removing the early transportable fraction of plutonium from lungs prior its absorption into blood. Translocation of DTPA from lungs to blood may also contribute to the decrease in extrapulmonary retention, as shown by reduced liver deposit after delayed pulmonary administration of DTPA. Efficacy of DTPA dry powder was further increased by the combined intravenous administration of DTPA solution for reducing extrapulmonary deposits of plutonium and promoting its urinary excretion. According to our results, the most effective treatment protocol for plutonium decorporation was the early pulmonary delivery of DTPA powder supplemented by an intravenous injection of DTPA solution. Following inhalation of plutonium as nitrate chemical form, this combined chelation therapy should provide a more effective method of treatment than conventional intravenous injection alone. At later stages following lung contamination, pulmonary administration of DTPA should also be considered as the treatment of choice for decreasing the lung burden.

  8. Effect of DTPA on the nephrotoxicity induced by uranium in the rat

    Energy Technology Data Exchange (ETDEWEB)

    Houpert, P.; Muller, D.; Chazel, V.; Claraz, M.; Paquet, F

    2003-07-01

    The only treatment proposed after human contamination with MOX (mixed oxide of uranium and plutonium) is diethylenetriaminepentaacetic acid (DTPA), because plutonium is considered to be the major risk. However, both DTPA and uranium are nephrotoxic at high dosages and DTPA has been shown to increase in vitro the cytotoxicity induced by uranium on cultured epithelial tubular cells. This work aimed to test this effect in vivo. Rats were injected with subtoxic (57 {mu}g.kg{sup -1}) to toxic (639 {mu}g kg{sup -1}) amounts of uranium as nitrate at 0 h, they received two DTPA injections (30 {mu}mol kg{sup -1}) at 2 min and 24 h and were euthanased at 48 h. The nephrotoxic effects were evaluated by measurement of the body weight gain, food and water intake, measurement of biochemical parameters in urine and blood, and histological examination of one kidney. The main result was that DTPA did not increase the nephrotoxicity induced by uranium in the range of concentrations tested, which was inconsistent with the in vitro results. (author)

  9. Easy and Efficient 111Indium Labeling of Long-Term Stored DTPA Conjugated Protein

    DEFF Research Database (Denmark)

    Nalla, Amarnadh; Buch, Inge; Hesse, Birger

    2011-01-01

    -diethylene-triamine-pentaacetic acid (cDTAP) was conjugated to a polyclonal immunoglobulin-G (IgG) in borate buffer, pH 8.2 at +4?C for 4 hours. Then the DTPA-conjugate was dialyzed against 50 mmol/l sodium citrate buffer saline, pH 6.0 and stored at -80° C in aliquots of 1 mg/0.5 ml. The DTPA-conjugate was labeled with 111In......-chloride in citrate buffer, pH 6. The labelling reaction was incubated at room temperature (RT) for 30 min and at +4?C for 90 min. Determination of labelling efficiency was performed using ITLC and an instant chromatography scanner equipped with a NaI crystal. The labelling efficiency of the DTPA...

  10. Synthesis and Characterization of 2-Decyl-DTPA and Its Gd(Ⅲ) Chelate

    Institute of Scientific and Technical Information of China (English)

    FENG Zhi-ming; LI Feng; CHEN Rong; ZHU Xiao-juan; LI Xiao-ru

    2004-01-01

    The present paper covers the synthesis and the characterization of ligand 2-decyl-3, 6, 9-tris(carboxymethyl)-3,6,9-triazaundecan-1,11-dioic acid, H5L, and its Gd(Ⅲ) chelate. The protonation constants for H5L(lgKHi=10.90, 8.50, 4.55, 2.92, 2.20) and the stability constant for GdL2- (lgKGdL2-=22.80) were determined by means of potentiometric titration. They are similar to the corresponding values of DTPA and Gd-DTPA, respectively. The results obtained show that the basicity of the ligand and the stability constant of its Gd(Ⅲ) chelate are not obviously altered after the introduction of a linear chain decyl group into the terminal acetic acid residue of DTPA. The Gd(Ⅲ) chelate may be a potential contrast agent with liver-specificity for magnetic resonance imaging(MRI).

  11. High-hardness ceramics based on boron carbide fullerite derivatives

    Science.gov (United States)

    Ovsyannikov, D. A.; Popov, M. Yu.; Perfilov, S. A.; Prokhorov, V. M.; Kulnitskiy, B. A.; Perezhogin, I. A.; Blank, V. D.

    2017-02-01

    A new type of ceramics based on the phases of fullerite derivatives and boron carbide B4C is obtained. The material is synthesized at a temperature of 1500 K and a relatively low pressure of 4 GPa; it has a high hardness of 45 GPa and fracture toughness of 15 MPa m1/2.

  12. Evaluation of Gd-DTPA-monophytanyl and phytantriol nanoassemblies as potential MRI contrast agents.

    Science.gov (United States)

    Gupta, Abhishek; de Campo, Liliana; Rehmanjan, Beenish; Willis, Scott A; Waddington, Lynne J; Stait-Gardner, Tim; Kirby, Nigel; Price, William S; Moghaddam, Minoo J

    2015-02-03

    Supramolecular self-assembling amphiphiles have been widely used in drug delivery and diagnostic imaging. In this report, we present the self-assembly of Gd (III) chelated DTPA-monophytanyl (Gd-DTPA-MP) amphiphiles incorporated within phytantriol (PT), an inverse bicontinuous cubic phase forming matrix at various compositions. The dispersed colloidal nanoassemblies were evaluated as potential MRI contrast agents at various magnetic field strengths. The homogeneous incorporation of Gd-DTPA-MP in PT was confirmed by polarized optical microscopy (POM) and synchrotron small-angle X-ray scattering (SAXS) of the bulk phases of the mixtures. The liquid crystalline nanostructures, morphology, and the size distribution of the nanoassemblies were studied by SAXS, cryogenic transmission electron microscopy (cryo-TEM), and dynamic light scattering (DLS). The dispersions with up to 2 mol % of Gd-DTPA-MP in PT retained inverse cubosomal nanoassemblies, whereas the rest of the dispersions transformed to liposomal nanoassemblies. In vitro relaxivity studies were performed on all the dispersions at 0.54, 9.40, and 11.74 T and compared to Magnevist, a commercially available contrast agent. All the dispersions showed much higher relaxivities compared to Magnevist at both low and high magnetic field strengths. Image contrast of the nanoassemblies was also found to be much better than Magnevist at the same Gd concentration at 11.74 T. Moreover, the Gd-DTPA-MP/PT dispersions showed improved relaxivities over the pure Gd-DTPA-MP dispersion at high magnetic fields. These stable colloidal nanoassemblies have high potential to be used as combined delivery matrices for diagnostics and therapeutics.

  13. Gelatin Nanofiber Matrices Derived from Schiff Base Derivative for Tissue Engineering Applications.

    Science.gov (United States)

    Jaiswal, Devina; James, Roshan; Shelke, Namdev B; Harmon, Matthew D; Brown, Justin L; Hussain, Fazle; Kumbar, Sangamesh G

    2015-11-01

    Electrospinning of water-soluble polymers and retaining their mechanical strength and bioactivity remain challenging. Volatile organic solvent soluble polymers and their derivatives are preferred for fabricating electrospun nanofibers. We report the synthesis and characterization of 2-nitrobenzyl-gelatin (N-Gelatin)--a novel gelatin Schiff base derivative--and the resulting electrospun nanofiber matrices. The 2-nitrobenzyl group is a photoactivatable-caged compound and can be cleaved from the gelatin nanofiber matrices following UV exposure. Such hydrophobic modification allowed the fabrication of gelatin and blend nanofibers with poly(caprolactone) (PCL) having significantly improved tensile properties. Neat gelatin and their PCL blend nanofiber matrices showed a modulus of 9.08 ± 1.5 MPa and 27.61 ± 4.3 MPa, respectively while the modified gelatin and their blends showed 15.63 ± 2.8 MPa and 24.47 ± 8.7 MPa, respectively. The characteristic infrared spectroscopy band for gelatin Schiff base derivative at 1560 cm(-1) disappeared following exposure to UV light indicating the regeneration of free NH2 group and gelatin. These nanofiber matrices supported cell attachment and proliferation with a well spread morphology as evidenced through cell proliferation assay and microscopic techniques. Modified gelatin fiber matrices showed a 73% enhanced cell attachment and proliferation rate compared to pure gelatin. This polymer modification methodology may offer a promising way to fabricate electrospun nanofiber matrices using a variety of proteins and peptides without loss of bioactivity and mechanical strength.

  14. Gd-Complexes of New Arylpiperazinyl Conjugates of DTPA-Bis(amides: Synthesis, Characterization and Magnetic Relaxation Properties

    Directory of Open Access Journals (Sweden)

    Abdullah O. Ba-Salem

    2015-04-01

    Full Text Available Two new DTPA-bis(amide based ligands conjugated with the arylpiperazinyl moiety were synthesized and subsequently transformed into their corresponding Gd(III complexes 1 and 2 of the type [Gd(LH2O]·nH2O. The relaxivity (R1 of these complexes was measured, which turned out to be comparable with that of Omniscan®, a commercially available MRI contrast agent. The cytotoxicity studies of these complexes indicated that they are non-toxic, which reveals their potential and physiological suitability as MRI contrast agents. All the synthesized ligands and complexes were characterized with the aid of analytical and spectroscopic methods, including elemental analysis, 1H-NMR, FT-IR, XPS and fast atom bombardment (FAB mass spectrometry.

  15. Organic Field-effect Transistors Based on Tetrathiafulvalene Derivatives

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    1 Restults Tetrathiafulvalene (TTF) and its derivatives have been extensively investigated in the field of organic conductors and superconductors since 1973. Recently, their application in organic field-effect transistors (OFETs) has attracted considerable attention. So far, on the one hand, the fabrication techniques of the TTF-based FETs have been primarily limited to high vacuum evaporation, which is a relatively expensive process. On the other hand, low FET performances, such as the low on/off ratio...

  16. Vesicoureteral Reflux Detected with 99mTc-DTPA Renal Scintigraphy during Evaluation of Renal Function

    Directory of Open Access Journals (Sweden)

    Nevena Manevska

    2015-12-01

    Full Text Available BACKGROUND: Radionuclide techniques, as direct radionuclide cystography and 99mTc-DMSA scintigraphy, have been used in evaluation of vesicoureteral reflux (VUR and reflux nephropathy (RN in children. Dynamic 99mTc-DTPA scintigraphy is reserved for evaluation of differential renal function and obstruction in children, where hydronephrosis is detected by ultrasonography (US pre- or postnatally. CASE REPORT: Six year old boy was prenatally diagnosed with bilateral hydronephrosis. Postnatal, severe bilateral VUR was detected by voiding urethrocytography. US and 99mTc-DTPA scintigraphy performed in the first month of life showed small left kidney that participated with 2% in the global renal function. Bilateral cutaneous ureterostomy has been performed in order to obtain good renal drainage and promote optimal renal growth. Twelve months later, classic antireflux procedure was done. Control 99mTc-DTPA scintigraphy, 5 ys after antireflux surgery, revealed persisting radioactivity during the diuretic phase, in the left kidney that indicated antireflux procedure failure with VUR reappearance. CONCLUSION: 99mTc-DTPA scintigraphy is the first method of choice for long-term monitoring of individual kidney function in children with VUR and other congenital urinary tract anomalies. Additionally, it can be used as indirect radionuclide cystography when rising of radioactivity in the kidney region, during the diuretic phase can indicate presence of VUR.

  17. Vesicoureteral Reflux Detected with 99mTc-DTPA Renal Scintigraphy during Evaluation of Renal Function

    Science.gov (United States)

    Manevska, Nevena; Stojanoski, Sinisa; Majstorov, Venjamin; Pop-Gjorcheva, Daniela; Zdraveska, Nikolina; Kuzmanovska, Dafina

    2016-01-01

    BACKGROUND: Radionuclide techniques, as direct radionuclide cystography and 99mTc-DMSA scintigraphy, have been used in evaluation of vesicoureteral reflux (VUR) and reflux nephropathy (RN) in children. Dynamic 99mTc-DTPA scintigraphy is reserved for evaluation of differential renal function and obstruction in children, where hydronephrosis is detected by ultrasonography (US) pre- or postnatally. CASE REPORT: Six year old boy was prenatally diagnosed with bilateral hydronephrosis. Postnatal, severe bilateral VUR was detected by voiding urethrocytography. US and 99mTc-DTPA scintigraphy performed in the first month of life showed small left kidney that participated with 2% in the global renal function. Bilateral cutaneous ureterostomy has been performed in order to obtain good renal drainage and promote optimal renal growth. Twelve months later, classic antireflux procedure was done. Control 99mTc-DTPA scintigraphy, 5 ys after antireflux surgery, revealed persisting radioactivity during the diuretic phase, in the left kidney that indicated antireflux procedure failure with VUR reappearance. CONCLUSION: 99mTc-DTPA scintigraphy is the first method of choice for long-term monitoring of individual kidney function in children with VUR and other congenital urinary tract anomalies. Additionally, it can be used as indirect radionuclide cystography when rising of radioactivity in the kidney region, during the diuretic phase can indicate presence of VUR. PMID:27275347

  18. Easy and Efficient 111Indium Labeling of Long-Term Stored DTPA Conjugated Protein

    DEFF Research Database (Denmark)

    Nalla, Amarnadh; Buch, Inge; Hesse, Birger

    2011-01-01

    targeting vehicles such as monoclonal antibodies and receptor directed peptides. The purpose of this study was to monitor the labelling efficiency of a DTPA-conjugate on long-term storage using 111In-chloride at two different temperatures and incubation times for the In-labelling. Method: Cyclic......-diethylene-triamine-pentaacetic acid (cDTAP) was conjugated to a polyclonal immunoglobulin-G (IgG) in borate buffer, pH 8.2 at +4?C for 4 hours. Then the DTPA-conjugate was dialyzed against 50 mmol/l sodium citrate buffer saline, pH 6.0 and stored at -80° C in aliquots of 1 mg/0.5 ml. The DTPA-conjugate was labeled with 111In...... months. Conclusion: Our study shows that 111In-labelling can easily be performed within 30 min at RT for thermo-stable proteins like polyclonal, DTPA-conjugated IgG stored long-term at -80°C with a high 111In-labelling efficiency....

  19. Easy and efficient (111)Indium labeling of long-term stored DTPA conjugated protein

    DEFF Research Database (Denmark)

    Nalla, Amarnadh; Buch, Inge; Hesse, Birger

    2011-01-01

    targeting vehicles such as monoclonal antibodies and receptor directed peptides. The purpose of this study was to monitor the labelling efficiency of a DTPA-conjugate on long-term storage using 111In-chloride at two different temperatures and incubation times for the In-labelling. Method: Cyclic......-diethylene-triamine-pentaacetic acid (cDTAP) was conjugated to a polyclonal immunoglobulin-G (IgG) in borate buffer, pH 8.2 at +4oC for 4 hours. Then the DTPA-conjugate was dialyzed against 50 mmol/l sodium citrate buffer saline, pH 6.0 and stored at -80o C in aliquots of 1 mg/0.5 ml. The DTPA-conjugate was labeled with 111In...... months. Conclusion: Our study shows that 111In-labelling can easily be performed within 30 min at RT for thermo-stable proteins like polyclonal, DTPA-conjugated IgG stored long-term at -80oC with a high 111In-labelling effi- ciency....

  20. Organic dyes based on fluorene and its derivatives

    Science.gov (United States)

    Kurdyukova, I. V.; Ishchenko, Aleksandr A.

    2012-03-01

    Data on various types of organic dyes based on fluorene and its derivatives, including polymethine, styryl, triphenylmethane, spiran, merocyanine, porphyrin and polymeric dyes, as well as azo dyes and donor-acceptor polyenes, are described systematically. The key methods for their synthesis are considered. The properties of the dyes are analyzed and summarized. The principles of development of modern functional materials based on these dyes are outlined. The use of these materials in advanced fields of science and technology such as photovoltaics, electroluminescence, nonlinear optics, holography, sensing photodynamic therapy are considered. The bibliography includes 476 references.

  1. Evaluation of nuclear imaging for detecting posttransplant fluid collection. [Comparison of /sup 99/Tc-DTPA imaging and ultrasonography for detection of fluid accumulation or lymphoceles

    Energy Technology Data Exchange (ETDEWEB)

    Burt, R.W. (Indiana Univ. Medical Center, Indianapolis); Reddy, R.K.

    1979-07-01

    A series of 26 renal allograft recipients studied by both B-mode gray scale ultrasonography and /sup 99m/Tc-DTPA renal nuclear imaging was reviewed. All conclusions were based on written reports generated at the original examination, and B-mode gray scale ultrasonography was considered the definitive examination for fluid collection detection. Sensitivity of nuclear imaging for the detection of fluid was 73%; however, it becomes 85% when fluid collections in close proximity to the bladder are excluded. Renal allograft nuclear imaging is a useful examination for primary detection of lymphoceles and also for demonstrating abnormalities in renal dynamics and drainage.

  2. Nanoporous Carbide-Derived Carbon Material-Based Linear Actuators

    Directory of Open Access Journals (Sweden)

    Janno Torop

    2009-12-01

    Full Text Available Devices using electroactive polymer-supported carbon material can be exploited as alternatives to conventional electromechanical actuators in applications where electromechanical actuators have some serious deficiencies. One of the numerous examples is precise microactuators. In this paper, we show for first time the dilatometric effect in nanocomposite material actuators containing carbide-derived carbon (CDC and polytetrafluoroetylene polymer (PTFE. Transducers based on high surface area carbide-derived carbon electrode materials are suitable for short range displacement applications, because of the proportional actuation response to the charge inserted, and high Coulombic efficiency due to the EDL capacitance. The material is capable of developing stresses in the range of tens of N cm-2. The area of an actuator can be dozens of cm2, which means that forces above 100 N are achievable. The actuation mechanism is based on the interactions between the high-surface carbon and the ions of the electrolyte. Electrochemical evaluations of the four different actuators with linear (longitudinal action response are described. The actuator electrodes were made from two types of nanoporous TiC-derived carbons with surface area (SA of 1150 m2 g-1 and 1470 m2 g-1, respectively. Two kinds of electrolytes were used in actuators: 1.0 M tetraethylammonium tetrafluoroborate (TEABF4 solution in propylene carbonate and pure ionic liquid 1-ethyl-3-methylimidazolium trifluoromethanesulfonate (EMITf. It was found that CDC based actuators exhibit a linear movement of about 1% in the voltage range of 0.8 V to 3.0 V at DC. The actuators with EMITf electrolyte had about 70% larger movement compared to the specimen with TEABF4 electrolyte.

  3. MR imaging of tumor angiogenesis using sterically stabilized Gd-DTPA liposomes targeted to CD105

    Energy Technology Data Exchange (ETDEWEB)

    Zhang Dong [Department of Radiology, XinQiao Hospital, Third Military Medical University, ChongQing 400037 (China); Feng Xiaoyuan [Department of Radiology, Hua Shan Hospital, Medical Center of FuDan University, ShangHai 200040 (China); Henning, Tobias D. [UCSF, Department of Radiology, Contrast Media Laboratory, 185 Berry Street, San Francisco, CA 94107 (United States); Wen Li [Department of Radiology, XinQiao Hospital, Third Military Medical University, ChongQing 400037 (China); Lu Weiyue; Pan Hong [Department of Pharmaceutical Targeting, Institute of Pharmacy, Medical Center of FuDan University, ShangHai 200032 (China); Wu Xing [Department of Neurosurgery, Hua Shan Hospital, Medical Center of FuDan University, ShangHai 200040 (China); Zou Liguang [Department of Radiology, XinQiao Hospital, Third Military Medical University, ChongQing 400037 (China)], E-mail: cqzdwl@yahoo.com.cn

    2009-04-15

    Aim: To depict tumor angiogenesis via the expression of CD105 in tumor-bearing rats using Gd-DTPA liposomes targeted to CD105 (CD105-Gd-SLs) on MR imaging. Materials and methods: Three Gd-DTPA liposomal nanoparticles were prepared in our trial: liposomes entrapping Gd-DTPA (Gd-SLs), Gd-SLs conjugated to immunoglobulins (IgG-Gd-SLs) and CD105-Gd-SLs. Forty glioma-bearing rats were randomized into four groups: (a) Gd-DTPA; (b) Gd-SLs; (c) IgG-Gd-SLs; (d) CD105-Gd-SLs. Axial T1WI MRI images were collected at baseline and repeated at 5, 30, 60 and 120 min post-intravenous injection of Gd-DTPA or liposome. Enhancement features and contrast-to-noise ratio of each group were analyzed. After imaging, tumors were resected for immunohistochemistry and immunofluorescence staining to assess vascularity and angiogenesis. Results: The four groups showed different enhancement features. The enhancement area was restricted for group CD105-Gd-SLs, while diffused for the other three. The degree of enhancement over time varied: group Gd-DTPA showed an early contrast enhancement at instant after injection with a peak at 30 min and a decline to baseline values at 60 min. In group CD105-Gd-SLs, the signal intensity (SI) continuously increased over 120 min. In groups IgG-Gd-SLs and Gd-SLs the SI peaked at 60 min, followed by a minor decrease for IgG-Gd-SLs and a rapid decrease for Gd-SLs almost to baseline. Immunohistochemistry and immunofluorescence showed that the enhancement in the CD105-Gd-SLs group resulted mainly from new microvessels. While in the other three groups, mature microvessels and new microvasculature resulted in the enhancement of the tumor. Conclusion: CD105-Gd-SLs can be used to detect early tumor angiogenesis on MR images. This might provide a means to non-invasively reveal a malignant phenotype of extracerebral F98 tumor and evaluate its progression.

  4. Radiation protect during the ventilation scintigraphy of Tc99m DTPA radioaerosol in pediatric application

    Energy Technology Data Exchange (ETDEWEB)

    Chen, Yu-Wen; Dai, Zen-Kong; Huang, Ying-Fong; Jong, Shiang-Bing [Chou-Hon Memorial Hospital, Kaohsiung Medical Univ., Kaohsiung, Taiwan (China)

    2000-05-01

    Lung ventilation-perfusion scintigraphy is of great value for the management of patients with both primary lung disease and heart disease, by proving patho- physiological information of importance for the diagnosis, follow-up and functional evaluation of the patients. Krypton 81m radioactive gas is preferable for pediatric application due to its short half-life. However, the rubidium-krypton 81m generator is not popular in hospital of our country. Tc99m DTPA radioaerosol ventilation scintigraphy has its unique convenient for clinical application. But, the most disadvantage of clinical application of Tc99m DTPA radioaerosol is contamination of environment when the poor-cooperative patient can't breathe by mouth. For this reason, we design the certain procedure to reduce the radioaerosol contamination. During May to Aug., 1999, we collect 36 pediatric patients (male to female ratio 2:1, age from 6 months to 20 years old) with clinical history of lung or heart disease, including congenital heart disease, asthma and so on. Before the cases receive 10 to 15 mCi Tc99m DTPA radioaerosol ventilation scan, all of them were trained with breath training. And during the ventilation scintigraphy, the special mouth mask is designed to prevent the radioaerosol leakage into atmosphere. Then Geiger-Muller survey meter was arranged to detect the environmental contamination of radioaerosol in the mask, one and two metes away from the mask every 10 minutes during ventilation scintigraphy procedure and 1 hour after finishing image. Two nuclear medicine physicians evaluated imaging quality of ventilation scintigraphy. Results: Among thirty-six pediatric patients with prior breath training, thirty-two cases are successful to proceed the Tc99m DTPA ventilation scintigraphy. The other four cases that were under three-year-old fail to receive ventilation scintigraphy. There is limited detectable radioactivity in the mouth mask at early 10 minute by Geiger-Muller counter. No significant

  5. Synthesis and Physicochemical Characterization of a Diethyl Ester Prodrug of DTPA and Its Investigation as an Oral Decorporation Agent in Rats.

    Science.gov (United States)

    Huckle, James E; Sadgrove, Matthew P; Leed, Marina G D; Yang, Yu-Tsai; Mumper, Russell J; Semelka, Richard C; Jay, Michael

    2016-07-01

    The increasing threats of nuclear terrorism have made the development of medical countermeasures a priority for international security. Injectable formulations of diethylenetriaminepentaacetic acid (DTPA) have been approved by the FDA; however, an oral formulation is more amenable in a mass casualty situation. Here, the diethyl ester of DTPA, named C2E2, is investigated for potential as an oral treatment for internal radionuclide contamination. C2E2 was synthesized and characterized using NMR, MS, and elemental analysis. The physiochemical properties of solubility, lipophilicity, and stability were investigated in order to predict its oral bioavailability. Finally, an animal efficacy study was conducted in Sprague Dawley rats pre-contaminated by intramuscular injection with (241)Am(NO3)3 to establish effectiveness of the therapy via the oral route. Synthesis of C2E2 yielded a crystalline powder with high solubility and improved lipophilicity over DTPA. The ester was stable in both simulated gastric and intestinal fluids over the anticipated time course of absorption. Capsules containing C2E2 were demonstrated to be stable for 12 months under accelerated stability conditions. After a single dose, C2E2 enhanced the elimination of (241)Am in a dose-dependent manner. Significant improvement was seen in both total (241)Am decorporation and reduction of (241)Am liver and skeletal burden. C2E2 was concluded to be effective when orally administered to (241)Am-contaminated rats. It may therefore have potential for medical countermeasure in treating humans contaminated with (241)Am or other transuranic elements. An oral capsule or powder for reconstitution may be suitable formulations for future development based on the physiochemical properties and anticipated dose required for efficacy.

  6. Single-molecular diodes based on opioid derivatives.

    Science.gov (United States)

    Siqueira, M R S; Corrêa, S M; Gester, R M; Del Nero, J; Neto, A M J C

    2015-12-01

    We propose an efficient single-molecule rectifier based on a derivative of opioid. Electron transport properties are investigated within the non-equilibrium Green's function formalism combined with density functional theory. The analysis of the current-voltage characteristics indicates obvious diode-like behavior. While heroin presents rectification coefficient R>1, indicating preferential electronic current from electron-donating to electron-withdrawing, 3 and 6-acetylmorphine and morphine exhibit contrary behavior, Rdiodes. In particular, the rectification rations for heroin diodes show microampere electron current with a maximum of rectification (R=9.1) at very low bias voltage of ∼0.6 V and (R=14.3)∼1.8 V with resistance varying between 0.4 and 1.5 M Ω. Once most of the current single-molecule diodes usually rectifies in nanoampere, are not stable over 1.0 V and present electrical resistance around 10 M. Molecular devices based on opioid derivatives are promising in molecular electronics.

  7. Dye-sensitized solar cells based on bisindolylmaleimide derivatives

    Institute of Scientific and Technical Information of China (English)

    Qiong ZHANG; Zhijun NING; Hongcui PEI; Wenjun WU

    2009-01-01

    Three organic dyes based on bisindolylmaleimide derivatives (11, 12 and 13) were synthesized and investigated as sensitizers for the application in nanocrystalline TiO2 solar cells. The indole group,maleimide group and carboxylic group functioned as electron donor, acceptor and anchoring group, respec-tively. Solar-to-electrical energy conversion efficiencies under simulated amplitude-modulated 1.5 irradiation based on 12 and of 1.87% and 1.50% for 13 and 11,respectively. The open circuit voltage Voc was demon-strated to be enhanced by the introduction of dodecyl or benzyl moieties on the indole groups. The nonplanar structure of bisindolylmaleimide was proven to be effective in aggregation resistance. This work suggests that organic sensitizers with maleimide as electron acceptor are promising candidates as organic sensiti-zers in dye-sensitized solar cells.

  8. Gadolinium-DTPA enhanced MRI with CHESS method for lumbar disc herniation; Visualization of affected nerve roots

    Energy Technology Data Exchange (ETDEWEB)

    Aota, Yoichi; Kumano, Kiyoshi; Hirabayashi, Shigeru; Ogawa, Yutaka; Yoshikawa, Koki (Kanto Rosai Hospital, Kawasaki, Kanagawa (Japan))

    1993-11-01

    A total of 34 DTPA-enhanced magnetic resonance imaging (MRI) studies were made pre- and/or post-operatively by using chemical shift selective method in 31 patients with lumbar disc herniation. DTPA enhancement was seen in affected nerve roots in 7 of 15 preoperative cases and 6 of 12 postoperative cases with unfavorable course. When straight leg raising test revealed severe stimulation to the lower extremity nerve roots (less than 45degree), affected nerve roots were significantly enhanced with DTPA. All enhanced sites, except for one of arachnoid inflammation, was most remarkably compressed by herniation. Edema within the affected nerve root would be detected by DTPA-enhanced MRI. (N.K.).

  9. Accuracy and confidence of Gd-EOB-DTPA enhanced MRI and diffusion-weighted imaging alone and in combination for the diagnosis of liver metastases

    Energy Technology Data Exchange (ETDEWEB)

    Donati, Olivio F.; Fischer, Michael A.; Chuck, Natalie; Hunziker, Roger [Institute of Diagnostic and Interventional Radiology, University Hospital Zurich, Zurich (Switzerland); Weishaupt, Dominik [Institute of Radiology, Triemli Hospital, Zurich (Switzerland); Reiner, Caecilia S., E-mail: caecilia.reiner@usz.ch [Institute of Diagnostic and Interventional Radiology, University Hospital Zurich, Zurich (Switzerland)

    2013-05-15

    Purpose: To evaluate the accuracy and confidence in diagnosing liver metastases using combined gadolinium-EOB-DTPA (Gd-EOB-DTPA) enhanced magnetic resonance imaging (MRI)/diffusion-weighted imaging (DWI) in comparison to Gd-EOB-DTPA enhanced MRI and DWI alone. Materials and methods: Forty-three patients (age, 58 ± 13 years) with 89 liver lesions (28 benign, 61 malignant) underwent liver MRI for suspected liver metastases. Three image sets (DWI, Gd-EOB-DTPA and combined Gd-EOB-DTPA/DWI) in combination with unenhanced T1- and T2-weighted images were reviewed by three readers. Detection rates of focal liver lesions were assessed and diagnostic accuracy was evaluated by calculating the areas under the receiver-operating-characteristics curve (AUC). Confidence in diagnosis was evaluated on a 3-point scale. Histopathology and imaging follow-up served as the standard of reference. Results: Detection of liver lesions and confidence in final diagnosis for all readers were significantly higher for the combined Gd-EOB-DTPA/DWI dataset than for DWI. The combination of DWI and Gd-EOB-DTPA rendered a significantly higher confidence in final diagnosis (2.44 vs. 2.50) than Gd-EOB-DTPA alone for one reader. For two readers, accuracy in diagnosis of liver metastases was significantly higher for Gd-EOB-DTPA/DWI (AUCs of 0.84 and 0.83) than for DWI datasets (AUCs of 0.73 and 0.72). Adding DWI to Gd-EOB-DTPA did not significantly increase diagnostic accuracy as compared to Gd-EOB-DTPA imaging alone. Conclusion: Addition of DWI sequences to Gd-EOB-DTPA enhanced MRI did not significantly increase diagnostic accuracy as compared to Gd-EOB-DTPA enhanced MRI alone in the diagnosis of liver metastases. However, the increase in diagnostic confidence might justify acquisition of DWI sequences in a dedicated MRI protocol.

  10. Glomerular filtration rate estimated from the uptake phase of 99mTc-DTPA renography in chronic renal failure

    DEFF Research Database (Denmark)

    Petersen, L J; Petersen, J R; Talleruphuus, U

    1999-01-01

    The purpose of the study was to compare the estimation of glomerular filtration rate (GFR) from 99mTc-DTPA renography with that estimated from the renal clearance of 51Cr-EDTA, creatinine and urea.......The purpose of the study was to compare the estimation of glomerular filtration rate (GFR) from 99mTc-DTPA renography with that estimated from the renal clearance of 51Cr-EDTA, creatinine and urea....

  11. Preclinical evaluation of (111)In-DTPA-INCA-X anti-Ku70/Ku80 monoclonal antibody in prostate cancer.

    Science.gov (United States)

    Evans-Axelsson, Susan; Vilhelmsson Timmermand, Oskar; Welinder, Charlotte; Borrebaeck, Carl Ak; Strand, Sven-Erik; Tran, Thuy A; Jansson, Bo; Bjartell, Anders

    2014-01-01

    The aim of this investigation was to assess the Ku70/Ku80 complex as a potential target for antibody imaging of prostate cancer. We evaluated the in vivo and ex vivo tumor targeting and biodistribution of the (111)In-labeled human internalizing antibody, INCA-X ((111)In-DTPA-INCA-X antibody), in NMRI-nude mice bearing human PC-3, PC-3M-Lu2 or DU145 xenografts. DTPA-conjugated, non-labeled antibody was pre-administered at different time-points followed by a single intravenous injection of (111)In-DTPA-INCA-X. At 48, 72 and 96 h post-injection, tissues were harvested, and the antibody distribution was determined by measuring radioactivity. Preclinical SPECT/CT imaging of mice with and without the predose was performed at 48 hours post-injection of labeled DTPA-INCA-X. Biodistribution of the labeled antibody showed enriched activity in tumor, spleen and liver. Animals pre-administered with DTPA-INCA-X showed increased tumor uptake and blood content of (111)In-DTPA-INCA-X with reduced splenic and liver uptake. The in vitro and in vivo data presented show that the (111)In-labeled INCA-X antibody is internalized into prostate cancer cells and by pre-administering non-labeled DTPA-INCA-X, we were able to significantly reduce the off target binding and increase the (111)In-DTPA-INCA-X mAb uptake in PC-3, PC-3M-Lu2 and DU145 xenografts. The results are encouraging and identifying the Ku70/Ku80 antigen as a target is worth further investigation for functional imaging of prostate cancer.

  12. Clearance of technetium-99m-DTPA and HRCT findings in the evaluation of patients with Idiopathic Pulmonary Fibrosis

    Directory of Open Access Journals (Sweden)

    Karkavitsas Nikolaos

    2006-02-01

    Full Text Available Abstract Background Clearance of inhaled technetium-labeled diethylenetriamine pentaacetate (99mTc-DTPA is a marker of epithelial damage and an index of lung epithelial permeability. The aim of this study was to investigate the role of 99mTc-DTPA scan in patients with Idiopathic Pulmonary Fibrosis (IPF. Our hypothesis is that the rate of pulmonary 99mTc-DTPA clearance could be associated with extent of High Resolution Computed Tomography (HRCT abnormalities, cell differential of bronchoalveolar lavage fluid (BALF and pulmonary function tests (PFTs in patients with IPF. Methods We studied prospectively 18 patients (14 male, 4 female of median age 67yr (range 55–81 with histologically proven IPF. HRCT scoring included the mean values of extent of disease. Mean values of these percentages represented the Total Interstitial Disease Score (TID. DTPA clearance was analyzed according to a dynamic study using a Venticis II radioaerosol delivery system. Results The mean (SD TID score was 36 ± 12%, 3 patients had mild, 11 moderate and 4 severe TID. Abnormal DTPA clearance half-time (t1/2 Conclusion Our data suggest that 99mTc-DTPA lung scan is not well associated with HRCT abnormalities, PFTs, and BALF cellularity in patients with IPF. Further studies in large scale of patients are needed to define the role of this technique in pulmonary fibrosis.

  13. Gadolinium-DTPA: value in MR imaging of extraspinal musculoskeletal infections

    Energy Technology Data Exchange (ETDEWEB)

    Haddad, M.C. [Dept. of Radiology, Riyadh Armed Forces Hospital, Riyadh (Saudi Arabia); Sharif, H.S. [Dept. of Radiology, Riyadh Armed Forces Hospital, Riyadh (Saudi Arabia); Aabed, M.Y. [Dept. of Radiology, Riyadh Armed Forces Hospital, Riyadh (Saudi Arabia); Al Shahed, M.S. [Dept. of Radiology, Riyadh Armed Forces Hospital, Riyadh (Saudi Arabia); Sammak, B.M. [Dept. of Radiology, Riyadh Armed Forces Hospital, Riyadh (Saudi Arabia); Clark, D.C. [Dept. of Radiology, Riyadh Armed Forces Hospital, Riyadh (Saudi Arabia)

    1993-12-01

    To determine if paramagnetic contrast agents can improve the detection, delineation, and characterization of extraspinal musculoskeletal infections (MSI) on magnetic resonance (MR) imaging, 42 patients with clinical suspicion of MSI underwent MR imaging before and after intravenous administration of gadolinium-DTPA. The lesions consisted of 27 proven infections and 15 noninfective conditions. Specificity and accuracy in identifying infective lesions averaged 80% and 84%, respectively, on precontrast studies and 80% and 89% on the enhanced examinations, with no statistically significant difference. Rim enhancement around abscess loculi was the only pathognomonic sign of infection seen in ten patients with chronic osteomyelitis and pyogenic or tuberculous infections. In 17 patients with acute osteomyelitis, brucellosis, or mycetoma, detection and delineation of the lesions were best on precontrast studies, while postcontrast examinations resulted in underestimation of the extent of abnormalities in all cases. We conclude that intravenous gadolinium-DTPA has limited usefulness in the MR evaluation of extraspinal MSI. (orig.)

  14. Calcium and zinc DTPA administration for internal contamination with plutonium-238 and americium-241.

    Science.gov (United States)

    Kazzi, Ziad N; Heyl, Alexander; Ruprecht, Johann

    2012-08-01

    The accidental or intentional release of plutonium or americium can cause acute and long term adverse health effects if they enter the human body by ingestion, inhalation, or injection. These effects can be prevented by rapid removal of these radionuclides by chelators such as calcium or zinc diethylenetriaminepentaacetate (calcium or zinc DTPA). These compounds have been shown to be efficacious in enhancing the elimination of members of the actinide family particularly plutonium and americium when administered intravenously or by nebulizer. The efficacy and adverse effects profile depend on several factors that include the route of internalization of the actinide, the type, and route time of administration of the chelator, and whether the calcium or zinc salt of DTPA is used. Current and future research efforts should be directed at overcoming limitations associated with the use of these complex drugs by using innovative methods that can enhance their structural and therapeutic properties.

  15. [177Lu]Bz-DTPA-EGF: Preclinical characterization of a potential radionuclide targeting agent against glioma.

    Science.gov (United States)

    Sundberg, Asa Liljegren; Gedda, Lars; Orlova, Anna; Bruskin, Alexander; Blomquist, Erik; Carlsson, Jörgen; Tolmachev, Vladimir

    2004-04-01

    Patients with glioblastoma multiforme have a poor prognosis due to recurrences originating from spread cells. The use of radionuclide targeting might increase the chance of inactivating single tumor cells with minimal damage to surrounding healthy tissue. As a target, overexpressed epidermal growth factor receptors (EGFR) may be used. A natural ligand to EGFR, the epidermal growth factor (EGF) is an attractive targeting agent due to its low molecular weight (6 kDa) and high affinity for EGFR. 177Lu (T(1/2) = 6.7 days) is a radionuclide well suited for treatment of small tumor cell clusters, since it emits relatively low-energy beta particles. The goal of this study was to prepare and preclinically evaluate both in vitro and in vivo the [177Lu]Bz-DTPA-EGF conjugate. The conjugate was characterized in vitro for its cell-binding properties, and in vivo for its pharmacokinetics and ability to target EGFR. [177Lu]Bz-DTPA-EGF bound to cultured U343 glioblastoma cells with an affinity of 1.9 nM. Interaction with EGFR led to rapid internalization, and more than 70% of the cell-associated radioactivity was internalized after 30 minutes of incubation. The retention of radioactivity was good, with more than 65% of the 177Lu still cell-associated after 2 days. Biodistribution studies of i.v. injected [177Lu]Bz-DTPA-EGF in NMRI mice demonstrated a rapid blood clearance. Most of the radioactivity was found in the liver and kidneys. The liver uptake was receptor-mediated, since it could be significantly reduced by preinjection of unlabeled EGF. In conclusion, [177Lu]Bz-DTPA-EGF seems to be a promising candidate for locoregional treatment of glioblastoma due to its high binding affinity, low molecular weight, and ability to target EGFR in vivo.

  16. Design of Ion-Exchange Resins Through EDTA and DTPA Modified Ligands

    Directory of Open Access Journals (Sweden)

    2014-07-01

    Catechol, resorcinol, and their admixtures with EDTA and DTPA moieties were converted into polymeric resins by alkaline polycondensation with formaldehyde. The resins were characterized by FTIR spectroscopy, elemental analysis, ion-exchange capacity, and distribution coefficient (D for heavy metal and radionuclide such as Cs and Sr. 137Cs and 90Sr constitutes a major source of heat in nuclear waste streams and in regards to recent nuclear event their remediation in complex solution – sea water - represent an important issue.

  17. Gd-DTPA enhancement of the facial nerve in Ramsay Hunt's syndrome

    Energy Technology Data Exchange (ETDEWEB)

    Kato, Tsutomu; Yanagida, Masahiro; Yamauchi, Yasuo (Kansai Medical School, Moriguchi, Osaka (Japan)) (and others)

    1992-10-01

    A total of 21 MR images in 16 Ramsay Hunt's syndrome were evaluated. In all images, the involved side of peripheral facial nerve were enhanced in intensity after Gd-DTPA. However, 2 cases had recovered facial palsy when MR images were taken. Nine of 19 cases with the enhancement of internal auditory canal portion had vertigo or tinnitus. Thus, it was suggested that the enhancement of internal auditory canal portion and clinical feature are closely related. (author).

  18. Effect of increased surface tension and assisted ventilation on /sup 99m/Tc-DTPA clearance

    Energy Technology Data Exchange (ETDEWEB)

    Jefferies, A.L.; Kawano, T.; Mori, S.; Burger, R.

    1988-02-01

    Experiments were performed to determine the effects of conventional mechanical ventilation (CMV) and high-frequency oscillation (HFO) on the clearance of technetium-99m-labeled diethylenetriamine pentaacetate (/sup 99m/Tc-DTPA) from lungs with altered surface tension properties. A submicronic aerosol of /sup 99m/Tc-DTPA was insufflated into the lungs of anesthetized, tracheotomized rabbits before and 1 h after the administration of the aerosolized detergent dioctyl sodium sulfosuccinate (OT). Rabbits were ventilated by one of four methods: 1) spontaneous breathing; 2) CMV at 12 cmH2O mean airway pressure (MAP); 3) HFO at 12 cmH2O MAP; 4) HFO at 16 cmH2O MAP. Administration of OT resulted in decreased arterial PO2 (PaO2), increased lung wet-to-dry weight ratios, and abnormal lung pressure-volume relationships, compatible with increased surface tension. /sup 99m/Tc-DTPA clearance was accelerated after OT in all groups. The post-OT rate of clearance (k) was significantly faster (P less than 0.05) in the CMV at 12 cmH2O MAP (k = 7.57 +/- 0.71%/min (SE)) and HFO at 16 cmH2O MAP (k = 6.92 +/- 0.61%/min) groups than in the spontaneously breathing (k = 4.32 +/- 0.55%/min) and HFO at 12 cmH2O MAP (4.68 +/- 0.63%/min) groups. The clearance curves were biexponential in the former two groups. We conclude that pulmonary clearance of /sup 99m/Tc-DTPA is accelerated in high surface tension pulmonary edema, and this effect is enhanced by both conventional ventilation and HFO at high mean airway pressure.

  19. Additional extrarenal abnormalities seen in Tc-99m DTPA renal flow study

    Energy Technology Data Exchange (ETDEWEB)

    Shih, Wei-Jen; Riley, C.; Domstad, P.A.; Pulmano, C.

    1988-04-01

    During /sup 99m/Tc-DTPA renal flow studies, extrarenal abnormalities have been found to include aortic abnormalities (aneurysm, ectasia, thrombosis, and abruptly decreased flow), splenic abnormalities (enlarged, small, or absent spleen), hepatic arterialization, and very slow circulation. In addition to the above abnormal findings, we add three more extrarenal pathologies that may be concomitantly found with renal flow study : pleural effusion(s), malignancy of the abdomen, and anemia and/or skeletal metastases.

  20. Hyaluronic Acid-Chitosan Nanoparticles to Deliver Gd-DTPA for MR Cancer Imaging

    Science.gov (United States)

    Zhang, Li; Liu, Tingxian; Xiao, Yanan; Yu, Dexin; Zhang, Na

    2015-01-01

    Molecular imaging is essential to increase the sensitivity and selectivity of cancer diagnosis especially at the early stage of tumors. Recently, polyionic nanocomplexes (PICs), which are composed of polyanions and opposite polycations, have been demonstrated to be a promising strategy for biomedical applications. In this work, chitosan-hyaluronic acid nanoparticles (GCHN) were developed to deliver Gd-DTPA as MRI contrast agents for tumor diagnosis. The Gd-labeled conjugates (CS-DTPA-Gd) were successfully synthesized by carbodiimide reaction, and then GCHN were prepared by ionic gelation using the obtained CS-DTPA-Gd and hyaluronic acid. The morphology of GCHN was spherical or ellipsoidal, which is observed by transmission electronic microscopy (TEM). The mean particle size and zeta potential of GCHN were 213.8 ± 2.6 nm and 19.92 ± 1.69 mV, respectively. The significant enhancement of signal intensity induced by GCHN was observed both in vitro and in vivo. Also, compared with Magnevist, GCHN was witnessed for a prolonged imaging time in the B16 tumor-bearing mice model. Furthermore, GCHN were verified as below toxic both in vitro and in vivo. These results indicated that GCHN could potentially be an alternative to current MRI contrast agents for tumor diagnosis.

  1. Preparation and initial characterization of biodegradable particles containing gadolinium-DTPA contrast agent for enhanced MRI

    Science.gov (United States)

    Doiron, Amber L.; Chu, Kevin; Ali, Adeel; Brannon-Peppas, Lisa

    2008-01-01

    Accurate imaging of atherosclerosis is a growing necessity for timely treatment of the disease. Magnetic resonance imaging (MRI) is a promising technique for plaque imaging. The goal of this study was to create polymeric particles of a small size with high loading of diethylenetriaminepentaacetic acid gadolinium (III) (Gd-DTPA) and demonstrate their usefulness for MRI. A water-in-oil-in-oil double emulsion solvent evaporation technique was used to encapsulate the MRI agent in a poly(lactide-co-glycolide) (PLGA) or polylactide-poly(ethylene glycol) (PLA-PEG) particle for the purpose of concentrating the agent at an imaging site. PLGA particles with two separate average sizes of 1.83 μm and 920 nm, and PLA-PEG particles with a mean diameter of 952 nm were created. Loading of up to 30 wt % Gd-DTPA was achieved, and in vitro release occurred over 5 h. PLGA particles had highly negative zeta potentials, whereas the particles incorporating PEG had zeta potentials closer to neutral. Cytotoxicity of the particles on human umbilical vein endothelial cells (HUVEC) was shown to be minimal. The ability of the polymeric contrast agent formulation to create contrast was similar to that of Gd-DTPA alone. These results demonstrate the possible utility of the contrast agent-loaded polymeric particles for plaque detection with MRI. PMID:18796605

  2. Evaluation of dynamic renal function by nuclear medicine using Tc-99m DTPA

    Energy Technology Data Exchange (ETDEWEB)

    Ishibashi, M.

    1987-01-01

    Dynamic renal function was nucleomedically evaluated using Tc-99m DTPA, and its value was assessed in various renal diseases. In addition to RI angiography, sequential image and renogram curve which were traditionally used as routine studies, Renal First Blood Flow (RFBF) by aortic blood flow exactly at the entrance of the renal artery, GFR (DTPA-GFR) by renal uptake and mean transit time (MTT) of the tracer by deconvolution analysis were measured. RFBF was useful to evaluate the stricture at the confluence of the renal artery and renal circulation, particularly cortical circulation. DTPA-GFR enabled the simple and rapid measurement of Total-GFR and Split-GFR. MTT was considered to be valuable in diagnosing obstructive nephropathy. In particular, RFBF was useful for assessment of renovascular hypertension. The measurement of the above-mentioned parameters made it possible to evaluate renal function objectively and quantitatively in addition to imaging and semiquantitative studies obtained by traditional routine examination. The major advantage of this procedure includes its possible performance along with routine renal function tests without any special preparation.

  3. Complexation of DTPA and EDTA with Cd(2+): stability constants and thermodynamic parameters at the soil-water interface.

    Science.gov (United States)

    Karak, Tanmoy; Paul, Ranjit Kumar; Das, Dilip Kumar; Boruah, Romesh Kumar

    2016-12-01

    Two alkaline soils collected from the surface horizon (0-15 cm) of two agricultural fields Lakshmikantapur (LKP; 22° 06' 03″ N and 88° 18' 19″ E) and Diamond Harbour (DHB; 22° 11' N and 88° 14' E) of West Bengal, India were studied to observe the stability of cadmium (Cd) chelate complexes with diethylenetriaminepentaacetatic acid (DTPA) and ethylenediaminetetraacetic acid (EDTA), removing organic matter (OM). The objective of the present study is "determination of the stability constants and the thermodynamic parameters of Cd-DTPA and Cd-EDTA complexes at different pH and temperatures at the soil-water interface". Complex formation of soil Cd with DTPA and EDTA at the soil-water interface was studied under different ligand-to-metal ratios, pHs and temperatures. Apparent conditional stability constants (log k´) were calculated from the concentrations of Cd chelates and free Cd(2+), estimated by solid phase extraction with an ion exchanger. Standard Gibbs energy (ΔG°), standard enthalpy (ΔH°) and standard entropy (ΔS°) of formation were calculated at three different temperatures. The higher stability constants of Cd-DTPA than Cd-EDTA indicated longer persistence of Cd-DTPA at the soil solution interface than Cd-EDTA complex. Increase of ΔG°, ΔH° and ΔS° with progress of temperature revealed that Cd-complex formation was facilitated by temperature. Highly negative ΔG° and positive ΔH° for Cd-complex formation indicated the reaction spontaneous and exothermic. In general, both ligands complexed high percentages of cadmium signalling their role in enhancing remobilization of Cd present in soil and preventing exchange of contaminated Cd from external source with soil mineral matrix; these phenomena may greatly reduce hazard for environment and human health. The result of this study support that DTPA increases solubility and more persistence of Cd in acidic soils within the range of temperature and mole fraction (MF = moles of Cd(2+)

  4. Development of {sup 68}Ga-labelled DTPA galactosyl human serum albumin for liver function imaging

    Energy Technology Data Exchange (ETDEWEB)

    Haubner, Roland [Innsbruck Medical University, Department of Nuclear Medicine, Innsbruck (Austria); Medizinische Universitaet Innsbruck, Universitaetsklinik fuer Nuklearmedizin, Innsbruck (Austria); Vera, David R.; Farshchi-Heydari, Salman [University of California, Department of Radiology, School of Medicine, and the UCSD Molecular Imaging Program, San Diego, CA (United States); Helbok, Anna; Rangger, Christine; Putzer, Daniel; Virgolini, Irene J. [Innsbruck Medical University, Department of Nuclear Medicine, Innsbruck (Austria)

    2013-08-15

    The hepatic asialoglycoprotein receptor is responsible for degradation of desialylated glycoproteins through receptor-mediated endocytosis. It has been shown that imaging of the receptor density using [{sup 99m}Tc]diethylenetriamine pentaacetic acid (DTPA) galactosyl human serum albumin ([{sup 99m}Tc]GSA) allows non-invasive determination of functional hepatocellular mass. Here we present the synthesis and evaluation of [{sup 68}Ga]GSA for the potential use with positron emission tomography (PET). Labelling of GSA with {sup 68}Ga was carried out using a fractionated elution protocol. For quality control thin-layer chromatography (TLC), high-performance liquid chromatography (HPLC) and size exclusion chromatography (SEC) techniques were evaluated. Stability of [{sup 68}Ga]GSA was studied in phosphate-buffered saline (PBS) and human serum. For in vivo evaluation [{sup 68}Ga]GSA distribution in Lewis rats was compared with [{sup 99m}Tc]GSA by using a dual isotope protocol. PET and planar imaging studies were performed using the same scaled molar dose of [{sup 68}Ga]GSA and [{sup 99m}Tc]GSA. Time-activity curves (TAC) for heart and liver were generated and corresponding parameters calculated (t50, t90). [{sup 68}Ga]GSA can be produced with high radiochemical purity. The best TLC methods for determining potential free {sup 68}Ga include 0.1 M sodium citrate as eluent. None of the TLC methods tested were able to determine potential colloids. This can be achieved by SEC. HPLC confirmed high radiochemical purity (>98 %). Stability after 120 min incubation at 37 C was high in PBS (>95 % intact tracer) and low in human serum ({proportional_to}27 % intact tracer). Biodistribution studies simultaneously injecting both tracers showed comparable liver uptake, whereas activity concentration in blood was higher for [{sup 68}Ga]GSA compared to [{sup 99m}Tc]GSA. The [{sup 99m}Tc]GSA TACs exhibited a small degree of hepatic metabolism compared to the [{sup 68}Ga]GSA curves. The mean

  5. Quantification of gadolinium-DTPA concentrations for different inversion times using an IR-turbo flash pulse sequence: a study on optimizing multislice perfusion imaging

    DEFF Research Database (Denmark)

    Fritz-Hansen, T; Rostrup, Egill; Ring, P B;

    1998-01-01

    The purpose of this study was to optimize an inversion-recovery (IR) turbo fast low-angle shot (FLASH) for multislice imaging by evaluating the accuracy of calculated the relaxation-rate (R1) for different inversion times (TI). This is important for tracer kinetic modeling because it requires...... a system responding linearly to input. R1 are linearly related to changes in the concentration of gadolinium (Gd)-diethylenetriaminepentaacetic acid (DTPA), and R1 is a parameter that can be derived from the magnetic resonance (MR) signal. The accuracy of calculated R1 using an IR turbo fast low-angle shot...... were similar, indicating that these curves, rather than signal curves, are more suitable even for qualitative perfusion evaluation. It is concluded that the results can be incorporated in a multislice IR turbo fast low-angle shot using the first slice (with a short TI) for assessment of both...

  6. 177Lu-DTPA-BIS-BIOTIN的制备及正常鼠体内生物分布%Preparation of 177Lu-DTPA-BIS-BIOTIN and Biodistribution Evaluation in Normal Mice

    Institute of Scientific and Technical Information of China (English)

    邓新荣; 杜进; 罗志福

    2010-01-01

    研究了DTPA-BIS-BIOTIN的177Lu标记方法,优化了标记条件,并进行了标记物在正常小鼠体内分布实验.在最佳标记条件下(DTPA-BIS-BIOTIN 25 μg,标记介质pH=4.5,80℃反应20 min),177Lu-DTPA-BIS-BIOTIN标记率大于99.0%,室温下放置96 h,标记物体外稳定性良好.正常小鼠体内分布实验结果表明,177Lu-DTPA-BIS-BIOTIN在血液中清除快,主要浓集于肝、脾和肾,经肾脏排泄.本研究为进一步采用177Lu-DTPA-BIS-BIOTIN进行肿瘤预定位显像及治疗研究提供了实验基础.

  7. Radar-Derived Quantitative Precipitation Estimation Based on Precipitation Classification

    Directory of Open Access Journals (Sweden)

    Lili Yang

    2016-01-01

    Full Text Available A method for improving radar-derived quantitative precipitation estimation is proposed. Tropical vertical profiles of reflectivity (VPRs are first determined from multiple VPRs. Upon identifying a tropical VPR, the event can be further classified as either tropical-stratiform or tropical-convective rainfall by a fuzzy logic (FL algorithm. Based on the precipitation-type fields, the reflectivity values are converted into rainfall rate using a Z-R relationship. In order to evaluate the performance of this rainfall classification scheme, three experiments were conducted using three months of data and two study cases. In Experiment I, the Weather Surveillance Radar-1988 Doppler (WSR-88D default Z-R relationship was applied. In Experiment II, the precipitation regime was separated into convective and stratiform rainfall using the FL algorithm, and corresponding Z-R relationships were used. In Experiment III, the precipitation regime was separated into convective, stratiform, and tropical rainfall, and the corresponding Z-R relationships were applied. The results show that the rainfall rates obtained from all three experiments match closely with the gauge observations, although Experiment II could solve the underestimation, when compared to Experiment I. Experiment III significantly reduced this underestimation and generated the most accurate radar estimates of rain rate among the three experiments.

  8. Multifunctional switches based on bis-imidazole derivative

    Indian Academy of Sciences (India)

    Abdullah M A Asiri; Gameel A Baghaffar; Khadija O Badahdah; Abdullah G M Al-Sehemi; Salman A Khan; Abeer A Bukhari

    2009-11-01

    multifunctional bis-imidazole derived from piperonal was prepared and found to have photo, thermo, solvato and peiezochromism with colour changes from pale green to deep blue. The multifunctionality colour changes and stability of the coloured species make the derivative candidates for various applications such as optical data storage. The photochromic properties and performance were found to be affected remarkably upon changing the solvent.

  9. Voltammetric characterization of montmorillonite-based organo derivatives

    OpenAIRE

    Navrátilová, Zuzana; Turčíková, Lenka; Plačková, Adriana

    2012-01-01

    Organo derivatives of clay mineral montmorillonite were prepared by modification of montmorillonite (type SAz-1) with three alkylammonium cations – hexadecyltrimethylammonium (HDTM), benzyldimethylhexadecylammonium (BDH), and hexadecylpyridinium bromide (HDP). The prepared organo-montmorillonites were characterized by X-Ray diffraction and infrared spectroscopy to test the alkylammonium cations presence in the organo derivatives. Multisweep cyclic voltammetry of copper at carbon paste e...

  10. Pharmacokinetic analysis of Gd-DTPA enhancement in dynamic MR of breast carcinoma. Pharmakokinetische Analyse der Gd-DTPA-Anreicherung in der MRT beim Mammakarzinom

    Energy Technology Data Exchange (ETDEWEB)

    Hess, T. (Deutsches Krebsforschungszentrum, Heidelberg (Germany). Forschungsschwerpunkt Radiologische Diagnostik und Therapie); Knopp, M.V. (Deutsches Krebsforschungszentrum, Heidelberg (Germany). Forschungsschwerpunkt Radiologische Diagnostik und Therapie); Hoffmann, U. (Deutsches Krebsforschungszentrum, Heidelberg (Germany). Forschungsschwerpunkt Radiologische Diagnostik und Therapie); Brix, G. (Deutsches Krebsforschungszentrum, Heidelberg (Germany). Forschungsschwerpunkt Radiologische Diagnostik und Therapie); Junkermann, H. (Heidelberg Univ. (Germany). Abteilung fuer Gynaekologische Radiologie); Zuna, I. (Deutsches Krebsforschungszentrum, Heidelberg (Germany). Forschungsschwerpunkt Radiologische Diagnostik und Therapie); Fournier, D. von (Heidelberg Univ. (Germany). Abteilung fuer Gynaekologische Radiologie); Kaick, G. van (Deutsches Krebsforschungszentrum, Heidelberg (Germany). Forschungsschwerpunkt Radiologische Diagnostik und Therapie)

    1994-06-01

    Dynamic Gd-DTPA enhanced MR of the breast was performed in one single slice in 27 patients with suspicious nodular lesions. The results could be histologically verified in all cases. A rapid spin-echo sequence with a time resolution of 8.75 s was used for the dynamic examination. The signal changes were analysed using a pharmacokinetic model which allowed parametrization of the contrast enhancement and transformation of the data into colour coded parameter images. The parameters allowed reliable distinction of 9 benign from 18 malignant lesions (p<0.05 for ''amplitude'', p<0.001 for ''k[sub 21]''). One fibroadenoma could not be distinguished from the carcinomas. Lymph node metastases and the pharmacokinetic parameter amplitude correlated significantly (p<0.05). (orig.)

  11. Effect of a plant origin drug on the biodistribution of 99mTc-DTPA in Wistar albino rats

    Directory of Open Access Journals (Sweden)

    Hasan Zora

    2012-04-01

    Full Text Available In recent years all over the world, medicinal plants are used quite a lot but side effects of biological and chemical contents and radiopharmaceutical interactions for each consumer in question aren't entirely well-known. The studies of plant origin drug interaction with radiopharmaceuticals are highly relevant and desired. One of them is passiflora syrup (Passiflora incarnata L., Passifloraceae which is widely used for depression, insomnia, anxiety and menopause period. The aim of current study is to evaluate possible effects of passiflora syrup on the biodistribution of 99mTc-DTPA and its blood cells uptake. DTPA was labeled with 99mTc radionuclide. Biodistribution studies were performed on male Wistar albino rats which were treated via oral feeding-gavage-method with either passiflora syrup or 0.9 % NaCl as control group for ten days. Blood samples were obtained by cardiac blood withdrawal from the rats and they were radiolabeled. The biodistribution results showed that the passiflora syrup decreased the uptake of 99mTc-DTPA in kidneys and in blood cells. 99mTc-DTPA being used widely as a kidney diagnostic agent in nuclear medicine seems to be interacting with orally taken passiflora. Passiflora syrup may modify the uptake of 99mTc-DTPA by kidney. The knowledge of this negative effect may contribute to reduce the risk of misdiagnosis and/or repetition of the examinations in nuclear medicine.

  12. Clinical phase II study with Gd-DTPA (dimeglumine gadopentetate, SHL 451 A) in brain tumor and cerebral infarction

    Energy Technology Data Exchange (ETDEWEB)

    Yoshikawa, Kohki; Aoki, Shigeki; Machida, Tohru and others

    1988-06-01

    A clinical phase II study with Gd-DTPA, the first contrast medium for MRI, was performed in 109 cases, a total of 114 studies mainly with brain tumor. In this study, one of the three concentrations of Gd-DTPA, 0.05 mmol/kg, 0.10 mmol/kg or 0.15 mmol/kg was used in each patient. It was elucidated that especially 0.10 and 0.15 Gd-DTPA were useful in detecting disruption or abscence of BBB and distinguishing tumor from edema. The side-effect observed was only a slight urticaria which was seen with 0.15 mmol/kg Gd-DTPA. Accordingly, it was estimated that the standard concentration of Gd-DTPA would be 0.10 mmol/kg. As this contrast medium was safe, and clinically useful in detecting diseases of the central nervous system, it was concluded that the shift to the clinical phase III trial is possible.

  13. Cadmium telluride detectors in the external measurement of glomerular filtration rate using 99mTc-DTPA(Sn): Comparison with /sup 51/Cr-EDTA and 99mTc-DTPA(Sn) plasma sample methods

    Energy Technology Data Exchange (ETDEWEB)

    Owen, J.E.; Walker, R.G.; D' Apice, A.J.F.; Willems, D.; Guignard, P.A.

    1982-01-01

    GFR was determined in 16 patients using an external detector to monitor disappearance of a single injected dose of 99mTc-DTPA (Sn) simultaneously with determinations of GFR using plasma sample methods for 99mTc-DTPA (Sn) and /sup 51/Cr-EDTA. Values of GFR were correlated closely between the external determinations of GFR and the plasma sample methods for /sup 51/Cr-EDTA and 99mTc-DTPA (Sn) with correlation coefficients of 0.97 and 0.99, respectively. Although the external detector method is apparently accurate, its advantages are as yet insufficient to warrant its adoption as the method of choice for determination of GFR.

  14. Technetium-99m DTPA inhalation scintigraphy in patients treated with fluoxetine and maprotiline: preliminary results

    Energy Technology Data Exchange (ETDEWEB)

    Kaya, G.C.; Durak, H.; Oezdogan, Oe.; Sayit, E.; Degirmenci, B.; Derebek, E. [Dokuz Eyluel Univ., Izmir (Turkey). School of Medicine; Yemez, B.; Turhal, Ue. [Dept. of Psychiatry, Dokuz Eylul Univ. School of Medicine, Izmir (Turkey)

    2000-09-01

    Drug-metabolising enzymatic activities have been detected in tracheobronchiolar, bronchiolar and alveolar regions in the lungs. Induction of phospholipidosis by amine drugs such as clorphentermine has also been shown. This study aimed to investigate the effect of fluoxetine and maprotiline, which contain amine groups in their structure, on pulmonary epithelial membrane permeability. Twenty-seven patients (mean age 36{+-}12 years) with various psychiatric problems, of whom 17 were treated with fluoxetine and 10 with maprotiline, were included in this study. Technetium-99m diethylene triamine penta-acetic acid (DTPA) aerosol inhalation scintigraphy was performed before and after 4-6 weeks of therapy. Following the inhalation of 1480 MBq {sup 99m}Tc-DTPA for 3 min, lung images in a 64 x 64 matrix were obtained every minute for 30 min. Regions of interest were drawn around the periphery of the lungs and on the major airways. Clearance half-times (T{sub 1/2}) were calculated by placing a mono-exponential fit on the curves. Penetration index (PI) was calculated on the first-minute image. There was no difference between the clearance rates of {sup 99m}Tc-DTPA before and after therapy for either the fluoxetine or the maprotiline group. After therapy, a significant decrease in PI was found in patients treated with fluoxetine (PI values before and after therapy: 0.53{+-}0.03 and 0.49{+-}0.05 respectively, P{<=}0.05). This finding might have been due to the induction of increased synaptic serotonin (5-HT) by fluoxetine, which acts by inhibiting the re-uptake of 5-HT on presynaptic membranes. Bronchoconstriction of small and medium airways may be caused by direct and indirect effects of 5-HT on smooth muscle contraction. (orig.)

  15. Lung clearance of {sup 99m} Tc- DTPA in systemic lupus erythematous

    Energy Technology Data Exchange (ETDEWEB)

    Dalcin, P.T.R; Barreto, S.S.M.; Xavier, R.M.; Brenol, J.C.T. [Rio Grande do Sul Univ., Porto Alegre, RS (Brazil). Hospital de Clinicas. Dept. de Medicina Interna; Cunha, R.D.; Marroni, B.J. [Rio Grande do Sul Univ., Porto Alegre, RS (Brazil). Hospital de Clinicas. Servico de Medicina Nuclear]. E-mail: rxavier@hcpa.ufrgs.br

    2002-06-01

    The early demonstration of lung involvement in systemic lupus erythematosus (SLE) patients is a difficult but important task. In the present study we attempted to identify abnormalities in pulmonary clearance of 99 mTc-DTPA in SLE, correlating their clearance data with clinical findings and disease activity. Forty-six consecutive SLE patients with and without active disease (LACC score) and 30 normal volunteers were studied. All subjects were submitted to pulmonary scintigraphy with 99 mTc-DTPA to evaluate the pulmonary clearance, and to a chest X-ray, and SLE patients were submitted to tests of disease activity, spirometry, arterial blood gases and tests to assess acute-phase proteins. Pulmonary clearance was faster in SLE patients with active disease when compared to normal controls [half-life of 67.04 min (51.52-82.55 min) in active SLE versus 85.87 min (78.85-92.87 min) in controls, P<0.05] and there was a higher frequency of abnormal clearance rates in patients with active disease (11 of 26 patients, 42.3%) when compared with SLE patients without disease activity (2 of 20 patients, 10%) (P = 0.04). A significant correlation was observed between the clearance rates and cough (P<0.05), but not between the clearance rates and dyspnoea symptoms or radiological findings, duration of SLE disease, antinuclear antibody titers and patterns, C-reactive protein or anti-double stranded DNA antibodies. We conclude that the pulmonary clearance of 99 m Tc-DTPA is increased in SLE patients with active disease. (author)

  16. Evaluation of renal function using Gd-DTPA dynamic MR imaging

    Energy Technology Data Exchange (ETDEWEB)

    Furukawa, Akira; Murata, Kiyoshi; Morita, Rikushi [Shiga Univ., Otsu (Japan). Medical Science

    1996-04-01

    To establish a new method for evaluating renal function using MRI, we performed dynamic Gd-DTPA enhanced MR imaging at 1.5 T in 68 subjects (71 examinations), including 23 normal volunteers, 19 patients with chronic renal failure, 22 with hydronephrosis, and 7 with renal vascular disorders. Thirty GRASS images [35/9/20deg/1 (TR/TE/Flip Angle/NEX)] were obtained after a bolus injection of Gd-DTPA (0.2 mmol/Kg) in each case, and were evaluated in the following respects. Whether or not the initial signal increase and/or the following signal drop in cortex was observed. Whether or not the signal drop in medulla and/or calyces was observed. Time between initial signal increase in cortex and signal drop in medulla (Ta). Time between signal drop in medulla and calyces (Tb). Time between signal increase in cortex and signal drop in calyces (Tc). Maximum ratio of signal intensity between cortex and medulla(Max. C/M). In normal subjects, the initial signal increase and the following signal drop in cortex, and the signal drop in medulla and calyces were observed. In patients with abnormal renal function, the characteristic signal changes discribed above were not seen clearly or disappeared in many cases. In normal subjects, Ta, Tb, Tc, and Max. C/M were 41.9{+-}6.9 sec, 53.2{+-}10.6 sec, 95.0{+-}9.5 sec, and 1.10{+-}0.05, respectively. In the patients, time parameters were significantly larger than those of normal subjects and C/M ratios were lower. This study suggested that dynamic Gd-DTPA renal MR imaging can serve as a new method for the evaluation of renal function. (author).

  17. Circulatory kinetics of intravenously injected {sup 238}Pu(IV) citrate and {sup 14}C-CaNa{sub 3}-DTPA in mice: Comparison with rat, dog, and Reference Man

    Energy Technology Data Exchange (ETDEWEB)

    Durbin, P.W.; Kullgren, B.; Schmidt, C.T. [Lawrence Berkeley National Lab., CA (United States)

    1997-02-01

    New ligands for in vivo chelation of Pu(IV) are being synthesized and evaluated in mice for efficacy and toxicity. Biokinetic studies of the new ligands, CaNa{sub 3}-DTPA, and Pu(IV) are major components of those investigations. Young adult female mice were injected intravenously (iv) with {sup 3}H-inulin, {sup 14}C-CaNa{sub 3}-DTPA, or {sup 238}Pu(IV) citrate to provide base- line data for plasma clearance, tissue uptake, and excretion rates and to determine the dilution volume (VOD) and renal clearance rate (RC) of filterable substances. Published plasma clearance data in Reference Man, dog, and rat were collected. Based on combined data for {sup 3}H-inulin and {sup 14}C-CaNa{sub 3}-DTPA, VOD = 17% of body weight and RC = 18 mL kg{sup -1} min{sup -1} for mice. Retention of {sup 14}C-CaNa{sub 3}-DTPA in the four species is proportional to body weight and inversely proportional to RC: Integrals of the retention of {sup 14}C-CaNa{sub 3}-DTPA from R(t) = 1.0 to R(t) = 0.05 are 108, 43, 28, and 10 DF min, respectively, for Reference Man, dog, rat, and mouse. Clearances of iv-injected Pu(IV) citrate from plasma are in the same order: The plasma curve integrals from injection to 1440 min are 840, 640, 280, and 67 DF min, respectively, for Reference Man, dog, rat, and mouse. In mice, a large fraction of newly injected Pu(IV) is rapidly transferred to the interstitial water of bulk soft tissue (excluding liver and kidneys), from which it is cleared at the same rate as from the plasma. Rapid plasma clearance, escape into interstitial water (22%ID at 20 min), significant early urinary excretion (8%ID in 12 h), and prompt deposition in liver and skeleton (complete in 12 h) are evidence of inefficient binding to plasma protein of newly injected Pu(IV) in mice. Slow plasma clearance, little early urinary excretion, and delayed deposition in liver and skeleton reflect more efficient binding of newly injected Pu(IV) in Reference Man and dog. 39 refs., 6 figs., 3 tabs.

  18. Development of [{sup 111}In]-DTPA-buserelin for GnRH receptor studies

    Energy Technology Data Exchange (ETDEWEB)

    Jalilian, A.R.; Akhlaghi, M.; Kamali-Dehghan, M.; Moradkhani, S. [Nuclear Medicine Research Group, Agricultural, Medical and Industrial Research School (AMIRS-NSTRI), Karaj (Iran); Shanehsazzadeh, S. [Dept. of Medical Physics, Tehran Univ. of Medical Sciences (Iran)

    2010-07-01

    Buserelin was successively labeled with [{sup 111}In]-Indium chloride after residuation with DTPA-dianhydride. Radio-chromatography showed an overall radiochemical purity of > 90% (RTLC) and > 95% (HPLC) at optimized conditions after labeling (specific activity: 400-450 GBq/M). The serum stability of the tracer was determined up to 24 h. The biodistribution of the tracer was determined in normal rats showing significant breast and ovaries uptakes 60 min post injection. MCF-7 cell binding of the tracer was > 98%. A significant testes uptake was demonstrated in normal male rats using SPECT studies. (orig.)

  19. Disseminated tuberculomas in spinal cord and brain demonstrated by MRI with gadolinium-DTPA

    Energy Technology Data Exchange (ETDEWEB)

    Shen, W.C. (Dept. of Radiology, Taichung Veterans General Hospital (Taiwan, Province of China)); Cheng, T.Y. (Section of Neurology, Dept. of Internal Medicine, Taichung Veterans General Hospital (Taiwan, Province of China)); Lee, S.K. (Dept. of Radiology, Taichung Veterans General Hospital (Taiwan, Province of China)); Ho, Y.J. (Dept. of Radiology, Taichung Veterans General Hospital (Taiwan, Province of China)); Lee, K.R. (Inst. of Life Science, National Tsing-Hua Univ. (Taiwan, Province of China))

    1993-03-01

    Intramedullary tuberculoma is rare, and there has been no report of concurrent intramedullary and intracerebral tuberculomas. We report a 30-year-old man with miliary tuberculosis of the lung. He suffered sudden paraplegia due to tuberculomas in the thoracic spinal cord and MRI showed more tuberculomas in the cervical spinal cord, brain stem, and cerebral and cerebellar hemispheres. The tuberculomas were isointense on the T1-weighted images, and hyperintense on the T2-weighted images; there was marked enhancement with intravenous gadolinium-DTPA. All the tuberculomas were very small 1 year after antituberculous chemotherapy. (orig.)

  20. Functional fecal retention visualized by (111)In-DTPA colon transit scintigraphy.

    Science.gov (United States)

    Infante, Jose Rafael; Rayo, Juan I; Serrano, Justo; Dominguez, Maria L; Garcia, Lucia; Moreno, Manuel

    2015-06-01

    Constipation is an extremely common problem in children, varying from mild and short-lived to severe and chronic. Chronic constipation is a serious childhood condition and requires further investigation, including blood test, colonoscopy, radio-opaque marker study, and/or scintigraphy. We present small bowel and colon transit scintigraphy of a 14-year-old girl with history of chronic constipation, abdominal pain, weight loss, and poor response to medical treatment. After oral administration of In-DTPA in water, planar and SPECT/CT images showed normal small bowel transit time and functional fecal retention in colon transit study.

  1. Extrarenal abnormalities in Tc-99m-DTPA renal blood flow studies

    Energy Technology Data Exchange (ETDEWEB)

    Shih, W.J.; Domstad, P.A.; DeLand, F.H.

    1985-01-01

    The authors observed extrarenal abnormalities during renal flow scintigraphy and retrospectively reviewed 90 patient studies to determine the types and frequencies of such abnormal findings. For each routine Tc-99m-DTPA renal flow study, they obtained nine 2-second sequential images, which included the heart, abdominal aorta, spleen and kidneys. Eighty abnormalities, observed in 62 patients, were divided into three categories: aortic, 37 cases; splenic, 40 cases; and miscellaneous, 3 cases. Other correlative studies including Tc-99m sulfur colloid-spleen scintigraphy, ultrasonography (US), CT, aortography, and surgical and/or autopsy findings were available for corroboration in 56 of 80 lesions.

  2. Simultaneous determination of DTPA, EDTA, and NTA by UV-visible spectrometry and HPLC.

    Science.gov (United States)

    Laine, Pirita; Matilainen, Rose

    2005-08-01

    In this study, UV-visible spectrophotometry (UV-Vis) and high-performance liquid chromatography (HPLC) were used for simultaneous analysis of chelating agents diethylenetriamine pentaacetic acid (DTPA), ethylenediamine tetraacetic acid (EDTA), and nitrilotriacetic acid (NTA), as their metal chelates in dishwashing detergents, natural waters, and pulp mill water. The total amounts of the chelating agents in dishwashing detergents were verified by potentiometric titration with Fe(III) solution. Nickel(II) chelates were determined by UV-Vis and iron(III)chelates by HPLC and titration. Recoveries of DTPA, EDTA, and NTA from a standard mixture of analytes by UV-Vis were 107+/-7, 101+/-12 and 94+/-13%, respectively, and the recovery of the total amount of complexing agents was 99+/-4%. The limits of detection for DTPA, EDTA, and NTA were 667, 324, and 739 micromol L(-1), respectively. In HPLC measurements the optimized mobile phase contained 0.03 mol L(-1) sodium acetate, 0.002 mol L(-1) tetrabutylammonium bromide, and 5% methanol at pH 3.15 and the detection was by UV-Vis detection at 254 nm. All three complexing agents could be separated from each other in a simultaneous analysis in less than 5 min. The limits of detection were 0.34, 0.27, and 0.62 micromol L(-1) for DTPA, EDTA, and NTA, respectively. The total amounts of the analytes measured in the dishwashing detergents by the three techniques were found to be highly comparable (ANOVA: F=0.04, P=0.96). R(2) values were 0.99 for EDTA, 0.99 for NTA, and 0.99 for all the results when UV-Vis and HPLC determinations were compared using regression lines. The UV-Vis and HPLC methods were proved to be viable also for analyses of natural and pulp mill waters. The absence of matrix interferences was verified by the standard addition technique.

  3. Comparative distribution study of /sup 111/In-labelled DTPA and TTHA monoclonal antibody conjugates in a choriocarcinoma xenograft model

    Energy Technology Data Exchange (ETDEWEB)

    Buckley, R.G.; Barnett, P.; Searle, F.; Pedley, R.; Boden, J.A.

    1986-11-01

    Conjugates of the chelating agents DTPA and TTHA with a monoclonal anti-HCG were prepared. The tissue distribution of the /sup 111/In-Labelled conjugates and also /sup 111/In-citrate was studied in mice bearing human choriocarcinoma xenografts. The antibody conjugates both gave high liver and spleen radionuclide accumulation. Elevated femur levels were observed for the TTHA conjugate and /sup 111/In-citrate. Generally the DTPA conjugate showed the highest tumor-tissue ratios, although its tumor/blood ratio was lower than the other two materials. The results infer that the DTPA conjugate has the greatest utility as an imaging agent but that it would require a background subtraction technique.

  4. Evaluation of (177)Lu-CHX-A''-DTPA-Bevacizumab as a radioimmunotherapy agent targeting VEGF expressing cancers.

    Science.gov (United States)

    Kameswaran, Mythili; Pandey, Usha; Gamre, Naresh; Vimalnath, K V; Sarma, Haladhar Dev; Dash, Ashutosh

    2016-08-01

    This study aimed at the preparation and evaluation of (177)Lu-CHX-A''-DTPA-Bevacizumab for targeting VEGF over-expressing cancers. Bevacizumab conjugated to p-NCS-Bn-CHX-A''-DTPA was radiolabeled with (177)Lu. The radioimmunoconjugate characterized by SE-HPLC exhibited radiochemical purity of 98.0±0.6%. In vitro stability was retained upto 4 days at 37°C. In vitro cell binding studies showed good uptake by VEGF expressing U937 tumor cells. Biodistribution studies in melanoma model showed significant uptake and retention of (177)Lu-CHX-A''-DTPA-Bevacizumab in tumor with reduction in uptake in presence of cold Bevacizumab confirming its specificity to VEGF.

  5. Quantitation of blood-brain barrier defect by magnetic resonance imaging and gadolinium-DTPA in patients with multiple sclerosis and brain tumors

    DEFF Research Database (Denmark)

    Larsson, H B; Stubgaard, M; Frederiksen, Jette Lautrup Battistini;

    1990-01-01

    In this study quantitation of the degree of deficiency of the blood-brain barrier (BBB) in patients with multiple sclerosis or brain tumors, by using MRI, is shown to be possible. As a measure of permeability of the BBB to Gadolinium-DTPA (Gd-DTPA) the flux per unit of distribution volume per unit...

  6. Spatial heterogeneity of DTPA-extractable zinc in cultivated soils induced by city pollution and land use

    Institute of Scientific and Technical Information of China (English)

    JIANG; Yong; LIANG; Wenju; WEN; Dazhong; ZHANG; Yuge; CHEN

    2005-01-01

    The spatial heterogeneity of DTPA-extractable zinc in the cultivated soils of Shenyang suburbs in Liaoning Province of China was investigated, and its map was drawn by the methods of geostatistics combined with geographic information system. The data of soil DTPA-extractable zinc fitted normal distribution after logarithm transformation, and its semivariogram fitted a spherical model. The semivariogram indicated that the spatial dependence of soil DTPA-extractable zinc content was moderate, with the spatial dependence range of 1.69 km and the fractal dimension of 1.96. Stochastic factors contributed to 49.9% of the spatial variability, while structural factors contributed to 50.1% of it. The spatial heterogeneity of soil DTPA-extractable zinc shown by a kriged interpolation map was deeply influenced by stochastic factors such as city pollution, land use pattern and crop distributions. For example, the average content of Zn in vegetable garden soils was 2.5-4 times as much as in their originated soils, and was lower in paddy soils than in their originated soils. The areas with a higher content of soil DTPA-extractable zinc appeared in the near suburbs and the riverside along Hunhe River and the wastewater drainage of Xihe River, and the extremely high values in the near suburb of the city's residential area were a striking feature, indicating the key role of city pollution in the spatial heterogeneity of soil DTPA-extractable zinc. When recorded in the form of a soil pollution map,the results of such a survey make it possible to identify the unusually polluted areas, and to provide more information for precise agriculture and environmental control.

  7. Evaluation of renal allograft dysfunction using {sup 99m}Tc-DTPA dynamic SPECT. Analysis with two compartment model and graph plot

    Energy Technology Data Exchange (ETDEWEB)

    Akahira, Hideaki; Takekawa, Shoichi; Nigawara, Kazuo; Funyu, Tomihisa [Oyokyo Kidney Research Inst., Hirosaki, Aomori (Japan). Hirosaki Hospital

    1996-12-01

    To estimate renal blood flow and glomerular function in transplanted kidneys, we applied the 2 compartment model and the graphic analysis method to {sup 99m}Tc-DTPA dynamic SPECT and calculated some parameters, i.e. K1 (renal influx rate constant), K3 (glomerular filtration rate constant), Vdl (function phase distribution volume), and others. Twenty-three renal transplant recipients were examined and divided into following 3 groups according to their serum creatinine levels (SCr); Group I; less than 1.3 mg/dl (1.1{+-}0.3, n=7), Group II: 1.4-2.5 mg/dl (1.8{+-}0.3, n=7), and Group III more than 2.6 mg/dl (4.8{+-}2.9, n=5). The K3 value became lower in the order of Group I>II>III, and well correlated with blood urea nitrogen (BUN, r=<0.91, P<0.001) and creatinine clearance (Ccr, r=0.87, P<0.001). The K1 value reduced markedly in Group III despite or no difference between Group I and II. Glomerular filtration rate (GFR) derived from K1 and K3 showed a correlation with those by Tauxe`s method. From these results and clinical conditions including histopathological findings, it is suggested that K1, K3 and Vdl are useful paraments of renal central arterial blood flow, renal peripheral arteriolar blood flow and renal {sup 99m}Tc-DTPA uptake function, respectively. (author)

  8. A simple and inexpensive system for controlling body temperature in small animal experiments using MRI and the effect of body temperature on the hepatic kinetics of Gd-EOB-DTPA.

    Science.gov (United States)

    Murase, Kenya; Assanai, Purapan; Takata, Hiroshige; Saito, Shigeyoshi; Nishiura, Motoko

    2013-12-01

    The purpose of this study was to develop a simple and inexpensive system for controlling body temperature in small animal experiments using magnetic resonance imaging (MRI) and to investigate the effect of body temperature on the kinetic behavior of gadolinium ethoxybenzyl diethylenetriamine pentaacetic acid (Gd-EOB-DTPA) in the liver. In our temperature-control system, body temperature was controlled using a feedback-regulated heated or cooled air flow generated by two Futon dryers. The switches of the two Futon dryers were controlled using a digital temperature controller, in which the rectal temperature of a mouse measured by an optical fiber thermometer was used as the input. In experimental studies, male ICR mice aged 8weeks old were used and allocated into 5 groups (39-, 36-, 33-, 30-, and 27-degree groups, n=10), in which the body temperature was maintained at 39 °C, 36 °C, 33 °C, 30 °C, and 27 °C, respectively, using our system. The dynamic contrast-enhanced MRI (DCE-MRI) data were acquired with an MRI system for animal experiments equipped with a 1.5-Tesla permanent magnet, for approximately 43min, after the injection of Gd-EOB-DTPA into the tail vein. After correction of the image shift due to the temperature-dependent drift of the Larmor frequency using the gradient-based image registration method with robust estimation of displacement parameters, the kinetic behavior of Gd-EOB-DTPA was analyzed using an empirical mathematical model. With the use of this approach, the upper limit of the relative enhancement (A), the rates of contrast uptake (α) and washout (β), the parameter related to the slope of early uptake (q), the area under the curve (AUC), the maximum relative enhancement (REmax), the time to REmax (Tmax), and the elimination half-life of the contrast agent (T1/2) were calculated. The body temperature of mice could be controlled well by use of our system. Although there were no significant differences in α, AUC, and q among groups, there

  9. Production of {sup 186}Re and {sup 188}Re, and synthesis of {sup 188}Re-DTPA

    Energy Technology Data Exchange (ETDEWEB)

    Hashimoto, Kazuyuki; Motoishi, Shoji; Kobayashi, Katsutoshi; Izumo, Mishiroku [Department of Radioisotopes, Japan Atomic Energy Research Institute, Tokai, Ibaraki (Japan); Musdja, Muhammad Yanis

    1999-08-01

    Production of radioactive rhenium isotopes {sup 186}Re and {sup 188}Re, and synthesis of {sup 188}Re-DTPA have been studied. For {sup 186}Re, a production method by the {sup 185}Re(n, {gamma}) {sup 186}Re reaction in a reactor has been established. For {sup 188}Re, a production method by the double neuron capture reaction of {sup 186}W, which produces a {sup 188}W/{sup 188}Re generator, has been established. For synthesis of {sup 188}Re-DTPA, the optimum conditions, including pH, the amounts of regents and so on, have been determined. (author)

  10. Transmetallation of Gd-DTPA by Fe3+, Cu2+ and Zn2+ in water: batch experiments and coagulation-flocculation simulations.

    Science.gov (United States)

    Rabiet, Marion; Letouzet, Marine; Hassanzadeh, Sepideh; Simon, Stéphane

    2014-01-01

    The study investigates the stability of gadolinium-DTPA complex in presence of competing metallic ions, Fe(3+), Cu(2+) and Zn(2+) using batch experiments and coagulation-flocculation simulations. High performance liquid chromatography with fluorescence detection was used for simultaneous analysis of chelate gadolinium (Gd-DTPA) and free Gd(III) ion in water. It was shown that Cu(2+) has a strong affinity for DTPA and could lead to a complete release of Gd(3+). Fe(3+) appeared also to compete strongly with Gd(3+) for DTPA binding since up to 80% of Gd-complex was dissociated under iron excess condition. Finally, zinc had a lower influence on Gd speciation: only 15% of Gd(3+) was released with addition of a 5-fold excess of Zn(2+). During coagulation-flocculation simulation, Fe(3+) was able to displace about 27% of Gd-DTPA, and no adsorption was observed onto flocs.

  11. 99Tcm标记Gd-DTPA-DG及其在裸鼠体内的生物分布%Radio-labeling of Gd-DTPA-DG with 99Tcm and its biodistribution in nude mice

    Institute of Scientific and Technical Information of China (English)

    黄占文; 张伟; 陈跃

    2011-01-01

    Objective To observe the stability and biodistribution of 99 Tcm radio-labeled a paramagnetic deoxy-glucose-type MR contrast agent diethylenetriamine pentaacetic acid-gadolinium salt deoxy-glucose(Gd-DTPA-DG) in tumor-bearing nude mice in vivo. Methods To form 99 Tcm-Gd-DTPA-DG , the labelling conditions such as Gd-DTPA-DG quality, SnCl2 · 2 H2 O dosage , the reaction medium pH value and reaction temperature were optimized by orthogonal experimental design. The radio-labeling efficiency was measured by thin layer chromatography(TLC). The bio-distribution was observed by the tumor and other major organs were taken out from nude mice at 10,30 min and 1, 2,4 , 24 h respectively after caudal vein injection of 99 Tcm-Gd-DTPA-DG. Results When taking 10 mg Gd-DTPA-DG,0. 6 mg SnCl2 · 2H2O,pH<2,adding 37 MBq Na99 TcmO4 ,then reacting in boiling water for 30 min, the radiochemical purity of 99Tcm-Gd-DTPA-DG can reach to 98. 5% and remain 96. 2% placed at room temperature for 6 h. 99TcmGd-DTPA-DG accumulated in the tumor to a higher level than other organ at 2 h,the uptake was about (1. 48±0. 12) %ID/g , and the radio-uptake ratio of tumor to muscle(T/NT)was reached 2. 91. Conclusion 99 Tcm labeled the paramagnetic MR contrast agent Gd-DTPA-DG is feasible. The labeled agent shows excellent tumor targeting in nude mice in vivo. 99 Tcm-Gd-DTPA-DG is potential for a SPECT(single photon emission computed tomography)-MRI dual-modality imaging probes.%目的 探讨锝(99Tcm)标记顺磁性脱氧葡萄糖类MRI对比剂二乙三胺五乙酸-脱氧葡萄糖钆盐(Gd-DTPA-DG)的稳定性及在荷瘤裸鼠体内的生物分布,寻找一种核素与磁共振双模式影像探针.方法 对 Gd-DTPA-DG进行99Tcm标记,并对需要的Gd-DTPA-DG、氯化亚锡(SnCl2·2H2O)用量,pH,温度采用正交实验设计,确定最佳标记条件.采用薄层纸层析法(TLC)分析标记率,体外放置6 h,观察标记物的稳定性.将标记的99Tcm -Gd-DTPA-DG注入裸鼠体内,10、30

  12. A triphenylamine-based benzoxazole derivative as a high-contrast piezofluorochromic material induced by protonation.

    Science.gov (United States)

    Xue, Pengchong; Chen, Peng; Jia, Junhui; Xu, Qiuxia; Sun, Jiabao; Yao, Boqi; Zhang, Zhenqi; Lu, Ran

    2014-03-11

    A triphenylamine-based benzoxazole derivative exhibits a low contrast piezofluorochromic behavior under external pressure, and a high-contrast fluorescence change induced by protonation can be observed.

  13. Diffuse liver disease: MRI with Gd-DTPA versus CT and Ultrasound

    Energy Technology Data Exchange (ETDEWEB)

    Vogl, T.J. [Dept. of Radiology, Univ. of Munich (Germany); Steiner, S. [Dept. of Radiology, Univ. of Munich (Germany); Schnell, B. [Dept. of Radiology, Univ. of Munich (Germany); Gerbes, A. [Dept. of Internal Medicine 2, Univ. of Munich (Germany); McMahon, C. [Dept. of Radiology, Univ. of Munich (Germany); Wilimzig, C. [Dept. of Radiology, Univ. of Munich (Germany); Lissner, J. [Dept. of Radiology, Univ. of Munich (Germany)

    1992-08-01

    Twenty-nine patients with diffuse liver disease were examined by ultrasound, CT and MRI. MRI was performed using T1- and T2-weighted spin-echo sequences as well as fast gradient-echo-sequences. The paramagnetic contrast agent Gd-DTPA was applied intravenously (0.1 mmol/kg). In patients with hepatitis, MRI could be used in guiding liver biopsies as inflammatory changes were clearly delineated. CT and ultrasound were superior to MRI in the detection of focal or diffuse fatty degeneration. On the other hand MRI was more helpful in differentiating fatty changes and neoplasm. In liver cirrhosis, fibrotic changes were most clearly demonstrated by MRI. In patients suffering from hemochromatosis MRI offers advantages over CT and ultrasound in the diagnosis and follow up due to the paramagnetic properties of iron, resulting in a reduction in signal intensity. In patients with Wilson`s disease a characteristic pattern of parenchymal changes was seen. Administration of Gd-DTPA contributes additional information about perfusion conditions in the liver parenchyma, however this information was not of diagnostic relevance in the cases we studied. (orig.)

  14. Utility of Gd-EOB-DTPA-Enhanced MRI in Diagnosing Small Hepatocellular Carcinoma

    Directory of Open Access Journals (Sweden)

    Soo Ryang Kim

    2009-07-01

    Full Text Available We describe an 8-mm hepatocellular carcinoma (HCC with hepatitis C virus-related cirrhosis in a 74-year-old woman. Ultrasound (US revealed an 8-mm hyperechoic nodule in segment 6 of the liver. Contrast-enhanced computed tomography (CT and US revealed no hypervascularity in the early phase and no washout in the late phase and the Kupffer phase, respectively. CT during arteriography revealed no hypervascularity and CT during arterial portography disclosed no perfusion defect. Gadolinium-ethoxybenzyl-diethylenetriamine pentaacetic acid (Gd-EOB-DTPA-enhanced magnetic resonance imaging (MRI revealed no hypervascularity in the early phase, but disclosed a defect in the hepatobiliary phase. Histologically, the nodule was diagnosed as well-differentiated HCC characterized by more than two-fold the cellularity of the non-tumorous area, with a high nuclear:cytoplasmic ratio, increased cytoplasmic eosinophilia, fatty change, and slight cell atypia with an irregular thin trabecular pattern. Our case demonstrates the utility of Gd-EOB-DTPA-enhanced MRI in the diagnosis of small HCC.

  15. Gadolinium-DTPA enhancement of symptomatic nerve roots in MRI of the lumbar spine

    Energy Technology Data Exchange (ETDEWEB)

    Tyrrell, P.N.M.; Cassar-Pullicino, V.N.; McCall, I.W. [Department of Diagnostic Imaging, The Institute of Orthopaedics, The Robert Jones and Agnes Hunt Orthopaedic and District Hospital NHS Trust, Oswestry, Shropshire SY10 7AG (United Kingdom)

    1998-02-01

    Disc prolapse presenting with sciatica may be associated with enhancement of the symptomatic nerve root following magnetic resonance imaging (MRI) with intravenous gadolinium (Gd)-DTPA. Previous studies have shown, however, that this does not occur in all cases. The aim of this study was to assess the incidence of nerve root enhancement in patients with sciatica and disc prolapse and to try to identify any specific features that might be associated with the phenomenon. A total of 227 patients presenting with low back pain and/or sciatica underwent a MRI study of the lumbar spine with intravenous contrast enhancement. Nineteen of 81 (23.5 %) patients with disc prolapse demonstrated nerve root enhancement. Nerve root enhancement had a highly significant association with sequestrated disc lesions (13/19, 68 %; P < 0.0005), and was primarily seen in the symptomatic ipsilateral nerve root (16/19, 84 %). The sensitivity of nerve root enhancement associated with disc prolapse was 23.5 % with a specificity of 95.9 %, a positive predictive value of 76 % and a negative predictive value of 69.3 %. Nerve root enhancement may be indicative of the symptomatic level but its poor sensitivity negates the routine use of Gd-DTPA in MRI for sciatica. (orig.) With 4 figs., 1 tab., 37 refs.

  16. Biological adhesive based on carboxymethyl chitin derivatives and chitin nanofibers.

    Science.gov (United States)

    Azuma, Kazuo; Nishihara, Masahiro; Shimizu, Haruki; Itoh, Yoshiki; Takashima, Osamu; Osaki, Tomohiro; Itoh, Norihiko; Imagawa, Tomohiro; Murahata, Yusuke; Tsuka, Takeshi; Izawa, Hironori; Ifuku, Shinsuke; Minami, Saburo; Saimoto, Hiroyuki; Okamoto, Yoshiharu; Morimoto, Minoru

    2015-02-01

    Novel biological adhesives made from chitin derivatives were prepared and evaluated for their adhesive properties and biocompatibility. Chitin derivatives with acrylic groups, such as 2-hydroxy-3-methacryloyloxypropylated carboxymethyl chitin (HMA-CM-chitin), were synthesized and cured by the addition of an aqueous hydrogen peroxide solution as a radical initiator. The adhesive strength of HMA-CM-chitin increased when it was blended with chitin nanofibers (CNFs) or surface-deacetylated chitin nanofibers (S-DACNFs). HMA-CM-chitin/CNFs or HMA-CM-chitin/S-DACNFs have almost equal adhesive strength compared to that of a commercial cyanoacrylate adhesive. Moreover, quick adhesion and induction of inflammatory cells migration were observed in HMA-CM-chitin/CNF and HMA-CM-chitin/S-DACNF. These findings indicate that the composites prepared in this study are promising materials as new biological adhesives.

  17. Vacina difteria, tétano e pertussis acelular (dTpa na gravidez: prevenção da tosse convulsa no lactente

    Directory of Open Access Journals (Sweden)

    Vera Amaral

    2015-09-01

    Full Text Available Introdução: Atualmente observa-se uma reemergência de tosse convulsa em países com elevadas coberturas vacinais, incluindo Portugal. Os lactentes com menos de três meses de idade constituem o grupo mais vulnerável. Novas estratégias vacinais têm sido propostas, entre as quais a vacinação no terceiro trimestre de gravidez. No entanto, a sua implementação é ainda limitada, existindo dúvidas quanto à sua eficácia e segurança. Objectivo: Rever a evidência quanto à eficácia e segurança da vacina difteria, tétano e pertussis acelular (dTpa administrada na gravidez na prevenção da tosse convulsa no lactente. Métodos: Foram pesquisadas normas de orientação clínica (NOC, revisões sistemáticas e estudos originais (EO na Medline, bases de dados de medicina baseada na evidência e sítios da Direção-Geral da Saúde e Sociedade Portuguesa de Pediatria. Utilizaram-se os termos MeSH pertussis vaccine e pregnancy e foram selecionados artigos publicados entre 01/07/09 e 15/07/2014 nas línguas portuguesa, inglesa e espanhola. Desenvolvimento: A maioria das NOC recomenda a vacinação de todas as grávidas no terceiro trimestre. Os EO mostram que a dTpa administrada na gravidez é eficaz no aumento da concentração de anticorpos anti-pertussis maternos e do lactente e na diminuição dos casos de doença e de hospitalizações em lactentes. A vacina não parece associar-se a aumento de efeitos adversos materno-fetais ou nas crianças. Conclusões: A vacinação com dTpa durante o terceiro trimestre de gravidez parece ser segura e eficaz na prevenção da tosse convulsa no lactente.

  18. Electrodynamic Analysis of Dissipative Electromagnetic Materials Based on Fractional Derivative

    Institute of Scientific and Technical Information of China (English)

    TAN Kang-Bo; LIANG Chang-Hong; DANG Xiao-Jie

    2007-01-01

    The generalized Lagrangian is defined in a dissipative electromagnetic medium on the basis of the combination of dynamical analysis and fractional derivative.Lorentz medium models are obtained by formulating relevant EulerLagrange equations.The invariance is obtained subsequently by investigating the invariance of time variation in the system,and then the relation between the related Hamiltonian and electromagnetic energy density is investigated.Canonical equations are obtained eventually.The electrodynamic interpretation on dissipative electromagnetic systems is revesled.

  19. Evaluation of three algorithms to calculate the relative renal function with {sup 99}Tc-DTPA; Evaluation de trois algorithmes pour calculer la fonction renale relative au {sup 99}Tc-DTPA

    Energy Technology Data Exchange (ETDEWEB)

    Charfeddine, S.; Maaloul, M.; Kallel, F.; Chtourou, K.; Guermazi, F. [EPS Habib Bourguiba, Service de Medecine Nucleaire, Sfax (Tunisia)

    2006-06-15

    The aim of our study is to estimate the reproducibility and the exactitude of three algorithms to determine with {sup 99m}Tc-DTPA the relative function of each kidney. Methods: a prospective study was carried out in voluntary patients. Reproducibility was studied in 11 patients who underwent had two examinations with {sup 99m}Tc-DTPA. Exactitude was evaluated in 35 patients who had an additional scintigraphy with {sup 99m}Tc-DMSA taken as a reference. To determine the relative renal function with {sup 99m}Tc-DTPA, three algorithms using various background subtraction methods and time intervals were applied. Results and conclusion: the method of the integral was the most reproducible and exact. It was little influenced by the choice of the interval of time. The reproducibility and the exactitude of the Patlak method were worse, especially in case of renal insufficiency or hydronephrosis. A high background and poor counting statistics explain why Patlak was less powerful with {sup 99m}Tc-DTPA than with {sup 99m}Tc-MAG3. The method of the slopes should not be recommended any more. (author)

  20. Liver hemangiona and metastasis: dynamic study with Gd-DTPA in magnetic resonance. Metastasis y hemangioma hepaticos: caracterizacion mediante estudio dinamico con Gd-DTPA en resonancia magnetica

    Energy Technology Data Exchange (ETDEWEB)

    Ferrer, M.D.; Marti-Bonmati, L.; Martinez-Rodrigo, J.; Galant, J. (Hospital ' ' Dr. Peset' ' . Servicio de Diagnostico por la imagen. Valencia (Spain))

    1993-05-01

    The difference between liver hemangioma and metastases is a subject of clinical interest. Nineteen MR liver studies are presented, 10 of them correspond to hemangiomas and 9 to metastases. In all cases, intravenous gadolinium was used in dynamic slices with T1-weighted gradient echo sequences. Late uptake is studied in two lesions. One minute postinjection, the periphery appears hyperintense (more so than the fatty tissue) in 7 out of the 10 cases of hemangioma; in 4 cases, the metastases show less intense uptake than fatty tissue. From two and a half minutes on, the periphery is hyperintense (more so than the fatty tissue) in all the hemangiomas, while in 4 metastases, the periphery is hyperintense (but less so than the fatty tissue), and the rest of the metastases remain hypo-isointense. Uptake by the hemangiomas at two hours reveals the isointense lesion with the liver. In conclusion, at approximately one minute postinjection, hemangiomas show very marked and nodular uptake, with centripetal progression; the hypersignal persists in late slices. However, the metastases present variable, moderate, irregular uptake at the beginning, with no central progression and reduced contrast in the late slices at 5 minutes. In some cases, Gd-DTPA increases the diagnostic certainty, improving reliability with respect to conventional MR. (Author)

  1. Platelet-Rich Blood Derivatives for Stem Cell-Based Tissue Engineering and Regeneration

    NARCIS (Netherlands)

    Masoudi, E.A.; Ribas, J.; Kaushik, G.; Leijten, J.C.H.; Khademhosseini, A.

    2016-01-01

    Platelet-rich blood derivatives have been widely used in different fields of medicine and stem cell-based tissue engineering. They represent natural cocktails of autologous growth factors, which could provide an alternative for recombinant protein-based approaches. Platelet-rich blood derivatives, s

  2. Separation characteristics of rare earth elements in the TOPO/DTPA - Salting-out reagent solution system

    Energy Technology Data Exchange (ETDEWEB)

    Miyahara, S.; Koma, Yoshikazu; Koyama, Tomozo; Tanaka, Yasumasa [Japan Nuclear Cycle Development Inst., Tokai, Ibaraki (Japan). Tokai Works; Kano, J.

    1998-11-01

    A test of mutual separation of rare earth elements was carried out using an organic phosphorus extraction solvent TOPO (tri-octyl phosphorus oxide). It was found that the distribution ratio monotonously increased with the atomic number and the separation factor of La/Ln was 10{sup 3}. Under the condition that a sufficient quantity of DTPA compared with the amount of rare earth metals and pH > 1.5 in which DTPA easily formed complexes with lanthanides, the following conclusions were obtained; (i) the separation factor was not affected by pH, the kind of salting-out reagent, or the concentration, (ii) the extraction reaction with TOPO and complex formation with DTPA mainly contributed to the separation of lanthanides, and (iii) the separation factor computed by means of the distribution ratio of TOPO extraction and complex formation constant for DTPA more or less agreed with the empirical value. Separation of rare earth elements using TOPO revealed similar characteristics to those of systems with CMPO and TBP. (H. Baba)

  3. Effect of desferrioxamine (DFO) and calcium trinatrium diethylenetriaminepentaacetic acid (DTPA) on rat cytomegalovirus replication in vitro and in vivo.

    Science.gov (United States)

    Kloover, J S; Scholz, M; Cinatl, J; Lautenschlager, I; Grauls, G E; Bruggeman, C A

    1999-11-01

    Cytomegalovirus (CMV) infection is a major problem in the immunosuppressed patient. It is thought that besides direct CMV induced cell lysis, immunological damage is part of CMV pathogenesis. New antiviral drugs, which combine immunomodulating and antiviral qualities, could be beneficial. Recently, it has been described that desferrioxamine (DFO) and calcium trinatrium diethylenetriaminepentaacetic acid (DTPA) exhibit both properties. In this report the antiviral effects of both compounds against rat CMV (RCMV) are described in vitro and in vivo using a generalised and local infection model. In vitro, both compounds exhibited a significant antiviral effect, DTPA being more potent than DFO. However, in the generalised infection model no effect was seen on mortality, morbidity or presence of virus in internal organs. In rats infected subcutaneously in the hind paw, no effect was seen locally on paw thickness, presence of viral antigens and inflammatory response. In addition, these rats suffered from a generalised infection of low magnitude at 15 days post infection, although both DFO and DTPA were able to lower the level of viral replication. In conclusion, our data indicate that despite in vitro activity, in vivo usage of DFO or DTPA for acute CMV infection is not warranted.

  4. Measurement of the arterial concentration of Gd-DTPA using MRI: a step toward quantitative perfusion imaging

    DEFF Research Database (Denmark)

    Fritz-Hansen, T; Rostrup, Egill; Larsson, H B

    1996-01-01

    A noninvasive method using an inversion recovery turbo-FLASH for dynamic measurement of the arterial input function represented by the bolus passage of Gd-DTPA in the descending aorta is presented, and the results are compared with the input function obtained by arterial blood samples. A good...

  5. Measurement of blood-brain barrier permeability using dynamic Gd-DTPA scanning--a comparison of methods

    DEFF Research Database (Denmark)

    Larsson, H B; Tofts, P S

    1992-01-01

    Two recently published methods of blood-brain barrier permeability measurement using Gd-DTPA scanning are compared by authors representing each group. The physiological models are reconciled. Results from both groups agree. These show that the transfer constant (the permeability surface area prod...

  6. Determination of kidney function with 99mTc-DTPA renography using a dual-head camera

    DEFF Research Database (Denmark)

    Madsen, Claus J; Møller, Michael L; Zerahn, Bo;

    2013-01-01

    Single-head gamma camera renography has been used for decades to estimate kidney function. An estimate of the glomerular filtration rate (GFR) can be obtained using Tc-diethylenetriaminepentaacetic acid (Tc-DTPA). However, because of differing attenuation, an error is introduced when the kidney...

  7. Dihydropyrimidine based hydrazine dihydrochloride derivatives as potent urease inhibitors.

    Science.gov (United States)

    Khan, Ajmal; Hashim, Jamshed; Arshad, Nuzhat; Khan, Ijaz; Siddiqui, Naureen; Wadood, Abdul; Ali, Muzaffar; Arshad, Fiza; Khan, Khalid Mohammed; Choudhary, M Iqbal

    2016-02-01

    Four series of heterocyclic compounds 4-dihydropyrimidine-2-thiones 7-12 (series A), N,S-dimethyl-dihydropyrimidines 13-18 (series B), hydrazine derivatives of dihydropyrimidine 19-24 (series C), and tetrazolo dihydropyrimidine derivatives 25-30 (series D), were synthesized and evaluated for in vitro urease inhibitory activity. The series B-D were first time examined for urease inhibition. Series A and C were found to be significantly active with IC50 values between 34.7-42.9 and 15.0-26.0 μM, respectively. The structure-activity relationship showed that the free S atom and hydrazine moiety are the key pharmacophores against urease enzyme. The kinetic studies of the active series A (7-12) and C (19-24) were carried out to determine their modes of inhibition and dissociation constants Ki. Compounds of series A (7-12) and series C (19-24) showed a mixed-type of inhibition with Ki values ranging between 15.76-25.66 and 14.63-29.42 μM, respectively. The molecular docking results showed that all the active compounds of both series have significant binding interactions with the active sites specially Ni-ion of the urease enzyme. Cytotoxicity of all series A-D was also evaluated against mammalian mouse fibroblast 3T3 cell lines, and no toxicity was observed in cellular model.

  8. Gd-EOB-DTPA-enhanced MR guidance in thermal ablation of liver malignancies.

    Directory of Open Access Journals (Sweden)

    Christian Rosenberg

    Full Text Available OBJECTIVE: To evaluate the potency of Gd-EOB-DTPA to support hepatic catheter placement in laser ablation procedures by quantifying time-dependent delineation effects for instrumentation and target tumor within liver parenchyma. Monitoring potential influence on online MR thermometry during the ablation procedure is a secondary aim. MATERIALS AND METHODS: 30 cases of MR-guided laser ablation were performed after i.v. bolus injection of gadoxetic acid (0.025 mmol/Kg Gd-EOB-DTPA; Bayer Healthcare, Berlin, Germany. T1-weighted GRE sequences were used for applicator guidance (FLASH 3D in the catheter placement phase and for therapy monitoring (FLASH 2D in the therapy phase. SNR and consecutive CNR values were measured for elements of interest plotted over time both for catheter placement and therapy phase and compared with a non-contrast control group of 19 earlier cases. Statistical analysis was realized using the paired Wilcoxon test. RESULTS: Sustainable signal elevation of liver parenchyma in the contrast-enhanced group was sufficient to silhouette both target tumor and applicator against the liver. Differences in time dependent CNR alteration were highly significant between contrast-enhanced and non-contrast interventions for parenchyma and target on the one hand (p = 0.020 and parenchyma and instrument on the other hand (p = 0.002. Effects lasted for the whole procedure (monitoring up to 60 min and were specific for the contrast-enhanced group. Contrasting maxima were seen after median 30 (applicator and 38 (tumor minutes, in the potential core time of a multineedle procedure. Contrast influence on T1 thermometry for real-time monitoring of thermal impact was not significant (p = 0.068-0.715. CONCLUSION: Results strongly support anticipated promotive effects of Gd-EOB-DTPA for MR-guided percutaneous liver interventions by proving and quantifying the delineating effects for therapy-relevant elements in the procedure. Time benefit, cost

  9. Decorporation of plutonium by pulmonary administration of Ca-DTPA dry powder: a study in rat after lung contamination with different plutonium forms

    Energy Technology Data Exchange (ETDEWEB)

    Serandour, A.L.; Grillon, G.; Frechou, M.; Fritsch, P.; Poncy, J.L. [CEA/DSV/DRR, Laboratoire de Radiotoxicologie, BP 12, 91680 Bruyeres-Le-Chatel (France); Tsapis, N.; Gervelas, C.; Fattal, E. [Universite Paris-Sud 11 CNRS, UMR 8612, Physico-chimie-Pharmacotechnie-Biopharmacie, 92296 Chatenay-Malabry (France); Deverre, J.R. [CEA/DSV/DRM, Service Hospitalier Frederic Joliot, 91401 Orsay (France); Benech, H. [CEA/DSV/DRM, Service de Pharmacologie et d' Immunologie, 91191, Gif-sur-Yvette (France)

    2007-07-01

    This study evaluates the decorporation efficacy of a pulmonary administration of a new Ca-DTPA (diethylene-triamine-pentaacetic acid) dry powder (18 {mu}mol kg{sup -1} of body mass) after pulmonary contamination of rats with different Pu compounds. After inhalation of PuO{sub 2}, a delayed intratracheal administration of DTPA cannot reduce significantly the retention of Pu in the lungs but limits its transfer in liver and skeleton. After pulmonary contamination by Pu nitrate, early insufflation of the DTPA powder appears twice as more efficient than an i.v injection of DTPA (30 {mu}mol kg{sup -1}) to reduce Pu retention in the lungs and is as effective as i.v. injection to limit the extra-pulmonary deposit. In contrast, a delayed administration of DTPA cannot reduce the lung or extra-pulmonary retention. In conclusion, the improvement of aerodynamic properties of DTPA powder leads to an increase of DTPA amount deposited in the lungs and enhances the body decorporation. (authors)

  10. Semi-automatic detection of Gd-DTPA-saline filled capsules for colonic transit time assessment in MRI

    Science.gov (United States)

    Harrer, Christian; Kirchhoff, Sonja; Keil, Andreas; Kirchhoff, Chlodwig; Mussack, Thomas; Lienemann, Andreas; Reiser, Maximilian; Navab, Nassir

    2008-03-01

    Functional gastrointestinal disorders result in a significant number of consultations in primary care facilities. Chronic constipation and diarrhea are regarded as two of the most common diseases affecting between 2% and 27% of the population in western countries 1-3. Defecatory disorders are most commonly due to dysfunction of the pelvic floor or the anal sphincter. Although an exact differentiation of these pathologies is essential for adequate therapy, diagnosis is still only based on a clinical evaluation1. Regarding quantification of constipation only the ingestion of radio-opaque markers or radioactive isotopes and the consecutive assessment of colonic transit time using X-ray or scintigraphy, respectively, has been feasible in clinical settings 4-8. However, these approaches have several drawbacks such as involving rather inconvenient, time consuming examinations and exposing the patient to ionizing radiation. Therefore, conventional assessment of colonic transit time has not been widely used. Most recently a new technique for the assessment of colonic transit time using MRI and MR-contrast media filled capsules has been introduced 9. However, due to numerous examination dates per patient and corresponding datasets with many images, the evaluation of the image data is relatively time-consuming. The aim of our study was to develop a computer tool to facilitate the detection of the capsules in MRI datasets and thus to shorten the evaluation time. We present a semi-automatic tool which provides an intensity, size 10, and shape-based 11,12 detection of ingested Gd-DTPA-saline filled capsules. After an automatic pre-classification, radiologists may easily correct the results using the application-specific user interface, therefore decreasing the evaluation time significantly.

  11. The efficiency of Gd-EOB-DTPA-enhanced magnetic resonance cholangiography in living donor liver transplantation: a preliminary study.

    Science.gov (United States)

    Ogul, Hayri; Kantarci, Mecit; Pirimoglu, Berhan; Karaca, Leyla; Aydinli, Bulent; Okur, Aylin; Ozturk, Gurkan; Kizrak, Yesim

    2014-03-01

    The aim of this study was to evaluate utility of gadoxetic acid disodium (Gd-EOB-DTPA)-enhanced magnetic resonance cholangiography (MRC) for the detection of biliary complications after living donor liver transplantation (LDLT). A total of 18 patients with suspected biliary complications underwent MRC. T2-weighted MRC and contrast-enhanced MRC (CE-MRC) were used to identify the biliary complications. MRC included routine breath-hold T2-weighted MRC using half-Fourier acquisition single-shot turbo spin-echo (HASTE) sequences and Gd-EOB-DTPA-enhanced MRC T1-weighted volumetric interpolated breath-hold examination (VIBE) sequences. Before confirming the biliary complications, one observer reviewed the MRC images and the CE-MRC images separately. The verification procedures and MRC findings were compared, and the sensitivity, specificity, and accuracy of both techniques were calculated for the identification of biliary complications. The observer found six of seven biliary complications using CE-MRC. The sensitivity was 85.7% and the accuracy was 94.4%. Using MRC alone, sensitivity was 57.1% and accuracy was 55.5%. The accuracy of Gd-EOB-DTPA-enhanced MRC was superior to MRC in locating biliary leaks (p < 0.05). The usage of Gd-EOB-DTPA-enhanced MRC yields information that complements the MRC findings that improve the identification of biliary complications. We recommend the use of MRC in addition to Gd-EOB-DTPA-enhanced MRC to increase the preoperative accuracy when assessing the biliary complications after LDLT.

  12. The advanced carbide-derived carbon based supercapacitor

    Science.gov (United States)

    Arulepp, M.; Leis, J.; Lätt, M.; Miller, F.; Rumma, K.; Lust, E.; Burke, A. F.

    The electrical double-layer (EDL) performance of three different TiC-derived nanoporous carbon materials was tested in prismatic capacitor assembly filled with 1.2 M triethylmethylammonium tetrafluoroborate (TEMA) acetonitrile solution. The electrical double-layer characteristics of supercapacitors were studied using the cyclic voltammetry (CV) and the electrochemical impedance spectroscopy (EIS) methods. Energy density versus power density, i.e. Ragone plots were constructed from the constant resistance and constant power (CP) charge/discharge data. The 1450F supercapacitor with novel nanoporous carbon made by halogen treatment of TiC/TiO 2 composite demonstrated the energy density of more than 10 Wh dm -3 at the cell voltage of 2.7 V.

  13. The advanced carbide-derived carbon based supercapacitor

    Energy Technology Data Exchange (ETDEWEB)

    Arulepp, M.; Leis, J.; Laett, M.; Miller, F.; Rumma, K. [Tartu Technologies Ltd., 185 Riia Str., 51014 Tartu (Estonia); Lust, E. [University of Tartu, 2 Jakobi Str., 51014 Tartu (Estonia); Burke, A.F. [University of California-Davis, 2003 Academic Surge, Davis, CA 95616 (United States)

    2006-11-22

    The electrical double-layer (EDL) performance of three different TiC-derived nanoporous carbon materials was tested in prismatic capacitor assembly filled with 1.2M triethylmethylammonium tetrafluoroborate (TEMA) acetonitrile solution. The electrical double-layer characteristics of supercapacitors were studied using the cyclic voltammetry (CV) and the electrochemical impedance spectroscopy (EIS) methods. Energy density versus power density, i.e. Ragone plots were constructed from the constant resistance and constant power (CP) charge/discharge data. The 1450F supercapacitor with novel nanoporous carbon made by halogen treatment of TiC/TiO{sub 2} composite demonstrated the energy density of more than 10Whdm{sup -3} at the cell voltage of 2.7V. (author)

  14. Antioxidant activity of N-acetyl-glucosamine based thiazolidine derivative

    Institute of Scientific and Technical Information of China (English)

    Li Chunlei; Yang Yan; Han Baoqin; Liu Wanshun

    2007-01-01

    N-acetyl-glucosamine,the monomer of chitin,was cyclo-condensed with L-cysteine to prepare thiazolidine derivative:2-N-acetyl-glucosamine-thiazolidine-4(R)-carboxylic acid(GlcNAcCys).The stability of GlcNAcCys was evaluated by high performance liquid chromatography(HPLC)measurement.The results showed that GlcNAcCys Was more stable than other TCA derivatives,especially in alkaline condition.The direct in vitro antioxidative properties of GlcNAcCys were investigated by using UV radiation-induced lipid peroxidation(LPO)in mitochondria and nuclei and.OH-induced LPO in red blood cell (RBC)ghosts models.UV radiation caused dose-dependent LPO in both mitochondria and nuclei,this effect Was catalvzed by addition of Fd2+ while prevented by co-incubation with GlcNAcCys.When nuclei and mitochondria Was treated with 100μl,300μl,500μl of GlcNAcCys and co-incubated at 37℃ for 30min,LPO was decreased to 96%,72%,68%in nuclei and 95%,72%,68% in mitochondria when compared to the UV radiation group respectively.Hydroxyl radicals(.OH)generated by Fenton reaction induced LPO in RBC ghosts.Pretreatment of RBC ghosts with GlcNAcCys could induce antioxidant RBC ghosts and inhibit concentration-dependent malondialdehyde(MDA)formation in antioxidant RBC ghosts.Its inhibition percent Was 14%,35%,36%,42%at 10,20,30,40ms/ml respectively.In a conclusion,the data suggest that GlcNAcCys has antioxidant ability and can significantly inhibit lipid peroxidation in biological samples tested in vitro.

  15. Stability of the four 2-(p-nitrobenzyl)-trans-CyDTPA {sup 88}Y complexes

    Energy Technology Data Exchange (ETDEWEB)

    Wu, C.; Brechbiel, M.W.; Gansow, O.A. [Radioimmune and Inorganic Chemistry Section, Radiation Oncology Branch, NCI, NIH, Bethesda, MD (United States); Kobayashi, H.; Carrasquillo, J. [Dept. of Nuclear Medicine, CC, NCI, NIH, Bethesda, MD (United States); Pastan, I. [Lab. of Molecular Biology, NCI, NIH, Bethesda, MD (United States)

    1997-12-31

    The unusual stereochemical influence on in vivo stability of the two C-Functionalized cyclohexyl diethylenetriamine-N,N,N`,N``,N``-pentaacetic acid (CyDTPA) chelating agents, (CHX-A, CHX-B), recently reported warranted further investigation to determine why such differences in configuration produce such striking effects on the stability of the Yttrium complex. To this end, all four individual component stereoisomers of CHX-A and CHX-B were synthesized for a detailed investigation into their chelation chemistry. Results of transchelation measurements, serum stability studies, and in vivo femur deposition measurements with {sup 88}Y indicate that the {sup 88}Y-CHX-A chelates are significantly more stable, in vitro and in vivo, than the {sup 88}Y-CHX-B complexes. Additionally, significant difference in vivo between the {sup 88}Y complexes formed from the component enantiomeric ligands were observed. (orig.)

  16. Tc-99m DTPA renal function tests and diuretic renogram in the dilated upper urinary tract

    Energy Technology Data Exchange (ETDEWEB)

    Sakagami, Yoshinari; Yamaguchi, Osamu; Suzuki, Takayuki; Kameoka, Hiroshi; Shiraiwa, Yasuo; Suzuki, Akira (Fukushima Medical Coll. (Japan))

    1992-09-01

    The authors studied patients with dilated upper urinary tracts (16 patients) using radioisotopic split renal function tests and diuretic renogram with [sup 99]mTc-diethylenetriaminepentaacetic acid (Tc-99m DTPA). The etiology was ureteropelvic function stenosis, 11; primary megaureter, 3; ureteral stenosis, 1; and aberrant vessel, 1. Response to diuresis was classified into 3 groups: i.e., obstructive pattern, non-obstructive pattern and partial obstructive pattern. In the non-obstructive group, split renal function was good, and thus an operation was not indicated. In the obstructive group, split renal function was significantly decreased and these patients underwent surgery. In the partial obstructive group, if function of the obstructed kidney was approximately the same as that of the non-obstructed side, then surgery was considered unnecessary. (author).

  17. Synthesis and biological assessment of folate-accepted developer (99m)Tc-DTPA-folate-polymer.

    Science.gov (United States)

    Chen, Fei; Shao, Kejing; Zhu, Bao; Jiang, Mengjun

    2016-05-15

    A novel cancer-targetable folate-poly(2-hydroxyethyl methacrylate) (PFDH) copolymer containing DTPA segment was prepared by conventional chemical synthesis and labeled with (99m)Tc subsequently. The (99m)Tc-labled PFDH could be produced easily with high radiochemical yield of 91% and radiochemical purity of 95%. The LogP octanol-water value for the (99m)Tc-labled PFDH was -2.19 and the radiotracer was stable in phosphate-buffered saline and human serum for 2h (>95% in PBS or ∼90% in human serum). To investigate (99m)Tc-labled PFDH tumor targeting, the in vitro and in vivo stability, cell uptake, in vivo biodistribution, and SPECT imaging were evaluated, respectively. These preliminary results strongly suggest that the novel folate conjugated dendrimer maybe developed to be potential for delivery of therapeutic radionuclides.

  18. Paramagnetic perfluorocarbon-filled albumin-(Gd-DTPA) microbubbles for the induction of focused-ultrasound-induced blood-brain barrier opening and concurrent MR and ultrasound imaging

    Science.gov (United States)

    Liao, Ai-Ho; Liu, Hao-Li; Su, Chia-Hao; Hua, Mu-Yi; Yang, Hung-Wei; Weng, Yu-Ting; Hsu, Po-Hung; Huang, Sheng-Min; Wu, Shih-Yen; Wang, Hsin-Ell; Yen, Tzu-Chen; Li, Pai-Chi

    2012-05-01

    This paper presents new albumin-shelled Gd-DTPA microbubbles (MBs) that can concurrently serve as a dual-modality contrast agent for ultrasound (US) imaging and magnetic resonance (MR) imaging to assist blood-brain barrier (BBB) opening and detect intracerebral hemorrhage (ICH) during focused ultrasound brain drug delivery. Perfluorocarbon-filled albumin-(Gd-DTPA) MBs were prepared with a mean diameter of 2320 nm and concentration of 2.903×109 MBs ml-1 using albumin-(Gd-DTPA) and by sonication with perfluorocarbon (C3F8) gas. The albumin-(Gd-DTPA) MBs were then centrifuged and the procedure was repeated until the free Gd3+ ions were eliminated (which were detected by the xylenol orange sodium salt solution). The albumin-(Gd-DTPA) MBs were also characterized and evaluated both in vitro and in vivo by US and MR imaging. Focused US was used with the albumin-(Gd-DTPA) MBs to induce disruption of the BBB in 18 rats. BBB disruption was confirmed with contrast-enhanced T1-weighted turbo-spin-echo sequence MR imaging. Heavy T2*-weighted 3D fast low-angle shot sequence MR imaging was used to detect ICH. In vitro US imaging experiments showed that albumin-(Gd-DTPA) MBs can significantly enhance the US contrast in T1-, T2- and T2*-weighted MR images. The r1 and r2 relaxivities for Gd-DTPA were 7.69 and 21.35 s-1mM-1, respectively, indicating that the MBs represent a positive contrast agent in T1-weighted images. In vivo MR imaging experiments on 18 rats showed that focused US combined with albumin-(Gd-DTPA) MBs can be used to both induce disruption of the BBB and detect ICH. To compare the signal intensity change between pure BBB opening and BBB opening accompanying ICH, albumin-(Gd-DTPA) MB imaging can provide a ratio of 5.14 with significant difference (p = 0.026), whereas Gd-DTPA imaging only provides a ratio of 2.13 and without significant difference (p = 0.108). The results indicate that albumin-(Gd-DTPA) MBs have potential as a US/MR dual-modality contrast agent for

  19. Immunoscintigraphy using /sup 111/In-DTPA labeled monoclonal antibodies: Comparison between ETC and planar imaging

    Energy Technology Data Exchange (ETDEWEB)

    Happ, J.; Baum, R.P.; Frohn, J.; Weimer, B.; Hoer, G.; Halbsguth, A.; Lochner, B.; Brandhorst, I.

    1987-12-01

    The present study was done in order to examine if the use of /sup 111/In-DTPA-labeled MAb fragments in place of /sup 131/I-labeled MAb fragments increases the sensitivity of tomographic immunoscintigraphy to reach the level of that of planar imaging techniques. In 11 patients with various primary tumors, local recurrences or metastases (colorectal carcinoma (n=7), ovarian carcinoma (n=2), papillary thyroid carcinoma (n=1), undifferentiated carcinoma of the lung (n=1)), immuniscintigraphy (IS) was carried out using /sup 111/In-DTPA-labeled F(ab')/sub 2/ fragments of various MAbs (anti-CEA, OC 125, anti-hTG) and planar and tomographic imaging were compared intraindividually. By conventional diagnostic procedures, the presence of a tumor mass was confirmed (transmission computer tomography, ultrasound) or verified (/sup 131/I whole-body scintigraphy, histology) in all cases. Immunoscintigraphy was positive in 9 out of 11 cases by ECT and in 10 out of 11 cases by planar imaging. When using /sup 111/In-labeled MAb fragments, intraindividual comparison of ECT and planar imaging resulted in a similar sensitivity. The increased sensitivity of ECT using this tracer in contrast to /sup 131/I-labeled MAb fragments may be attributed to the fact that the physical properties of /sup 111/In are much more suitable for the gamma cameras most commonly used (single detector, 38'' crystal); using /sup 111/In-labelled MAb fragments, count rates sufficient for ECT can be obtained within a reasonable acquisition time. This allows to combine IS with the advantages of ECT regarding tumour localization and prevention of artefacts due to superposition of background.

  20. MRT of scaphoid pseudo-arthrosis using Gd-DTPA. Staging and clinical correlation; MRT der Skaphoidpseudarthrose mit Gd-DTPA. Stadieneinteilung und klinische Korrelation

    Energy Technology Data Exchange (ETDEWEB)

    Vogl, T.J. [Freie Univ. Berlin (Germany). Strahlenklinik und Poliklinik; Beutel, F. [Freie Univ. Berlin (Germany). Strahlenklinik und Poliklinik; Wilhelm, K. [LMU Muenchen (Germany). Abt. fuer Handchirurgie; Tempka, A. [Freie Univ. Berlin (Germany). Klinik fuer Unfall- und Wiederherstellungschirurgie; Schedel, H. [Freie Univ. Berlin (Germany). Strahlenklinik und Poliklinik; Haas, R. [Freie Univ. Berlin (Germany). Strahlenklinik und Poliklinik; Felix, R. [Freie Univ. Berlin (Germany). Strahlenklinik und Poliklinik

    1994-11-01

    During a period of two years, 134 patients with pseudo-arthrosis of the scaphoid were examined by conventional radiography and by MRI in the course of a prospective study. The aim of the study was to define radiological staging using contrast enhanced MRI in order to improve the prognostic criteria. All MRI examinations were carried out with a 1.5 Tesla scanner (SP63) using a surface coil and T{sub 1} weighted spin echo sequences in sagittal and frontal projection and frontal FLASH T{sub 2}-sequences and axial spin echo T{sub 2} sequences. The T{sub 1} weighted SE sequences in frontal projection were carried out before and after iv contrast (0.1 mmol Gd-DTPA/kg KG). All sequences were compared with conventional radiographs and the operative findings. Eight patients in stage 0 showed high signal intensity of both fragments in T{sub 1} weighted SE sequences and at surgery there was good vascularisation. In 22 cases there was reduced signal intensity in at least one fragment (stage I). 45 patients with scaphoid pseudo-arthrosis showed complete signal loss but marked contrast uptake with still vital nuclei at surgery (stage II). In 22 patients, there was no increase in signal intensity after contrast and complete loss of vitality of the fragments at surgery. Staging was not possible in 37 patients because of previous operative intervention. The use of contrast enhanced MRI provides additional information compared with conventional radiography or plain MRI. (orig.) [Deutsch] Im Rahmen einer prospektiven Studie wurden in einem Zeitraum von 2 Jahren 134 Patienten mit einer Skaphoidpseudarthrose (SPA) vergleichend konventionell roentgenologisch und magnetresonanztomographisch untersucht. Ziel der Studie war die Erstellung einer klinisch radiologischen Stadieneinteilung mit Hilfe der kontrastverstaerkten MRT zur Verbesserung von Prognosekriterien. Alle MRT-Untersuchungen wurden an einem 1,5 Tesla-Geraet (SP63) mittels einer Oberflaechenspule unter Verwendung von T{sub 1

  1. Preparation of {sup 166} Dy/{sup 166} Ho DTPA-bis biotin as a system of In vivo generator; Preparacion de {sup 166} Dy/{sup 166} Ho DTPA-bis biotina como un sistema de generador In vivo

    Energy Technology Data Exchange (ETDEWEB)

    Jimenez V, M.R

    2003-07-01

    The objective of this work was to synthesize the complex {sup 166} Dy/{sup 166} Ho - diethylen triamine pentaacetic-bis Biotin ({sup 166} Dy/{sup 166} Ho DTPA-bis Biotin) to evaluate its potential as a new radiopharmaceutical in directed radiotherapy. The Dysprosium-166 was obtained for neutron irradiation of {sup 164} Dy{sub 2}0{sub 3} in the TRIGA Mark III reactor. The labelled was carried out in aqueous solution to p H 8.0 for addition of {sup 166} Dy Cl{sub 3} to the diethylen triamine pentaacetic-{alpha}, {omega}-bis Biotin (DTPA-bis Biotin). The radiochemical purity was determined for HPLC and ITLC. The biological integrity of the marked biotin is evaluated by the biological recognition of the avidin for HPLC - molecular exclusion with and without avidin addition. The studies of stability in vitro were made in dilutions of saline solution to 0.9% and with human serum at 37 C incubated 1 and 24 hours. The complex {sup 166} Dy/{sup 166} Ho DTPA-bis Biotin was obtained with a radiochemical purity of 99.1 {+-} 0.6%. The biological recognition of the complex {sup 166} Dy/{sup 166} Ho DTPA-bis Biotin for the avidin it doesn't affect the labelling procedure. The studies in vitro demonstrated that the {sup 166} Dy/{sup 166} Ho DTPA-bis Biotin is stable after the dilution in saline solution and in human serum that there is not translocation of the one radionuclide subsequent son to the beta decay of the {sup 166} Dy that could produce the {sup 166} Ho{sup 3+} liberation. The studies of Biodistribution in healthy mice demonstrated that the one complex {sup 166} Dy/{sup 166} Ho DTPA-bis Biotin have a high renal distribution. In conclusion the radiolabelled biotin in this investigation has the appropriate properties to be used as an In vivo generator system stable for directed radiotherapy. (Author)

  2. Analytical Derivation: An Epistemic Game for Solving Mathematically Based Physics Problems

    Science.gov (United States)

    Bajracharya, Rabindra R.; Thompson, John R.

    2016-01-01

    Problem solving, which often involves multiple steps, is an integral part of physics learning and teaching. Using the perspective of the epistemic game, we documented a specific game that is commonly pursued by students while solving mathematically based physics problems: the "analytical derivation" game. This game involves deriving an…

  3. Disk-like Tiles Derived from Complex Bases

    Institute of Scientific and Technical Information of China (English)

    Jun LUO; Zuo Ling ZHOU

    2004-01-01

    For each positive integer k, the radix representation of the complex numbers in the base-k + i gives rise to a lattice self-affine tile Tk in the plane, which consists of all the complex numbers that can be expressed in the form ∑j≥1 dj(-k + i)-j, where dj ∈ {0, 1,2,……,k2}. We prove that Tk is homeomorphic to the closed unit disk {z ∈ C: |z| ≤ 1} if and only if k ≠ 2.

  4. Intraindividual comparison of T1 relaxation times after gadobutrol and Gd-DTPA administration for cardiac late enhancement imaging

    Energy Technology Data Exchange (ETDEWEB)

    Doeblin, Patrick, E-mail: Patrick.doeblin@charite.de [Department of Cardiology, Charité – Universitätsmedizin Berlin, Charité Campus Benjamin Franklin, Berlin (Germany); Schilling, Rene, E-mail: rene.schilling@charite.de [Department of Radiology, Charité – Universitätsmedizin Berlin, Charité Campus Mitte, Berlin (Germany); Wagner, Moritz, E-mail: moritz.wagner@charite.de [Department of Radiology, Charité – Universitätsmedizin Berlin, Charité Campus Mitte, Berlin (Germany); Luhur, Reny, E-mail: renyluhur@yahoo.com [Department of Radiology, Charité – Universitätsmedizin Berlin, Charité Campus Mitte, Berlin (Germany); Huppertz, Alexander, E-mail: alexander.huppertz@charite.de [Department of Radiology, Charité – Universitätsmedizin Berlin, Charité Campus Mitte, Berlin (Germany); Imaging Science Institute, Charité, Berlin (Germany); Hamm, Bernd, E-mail: bernd.hamm@charite.de [Department of Radiology, Charité – Universitätsmedizin Berlin, Charité Campus Mitte, Berlin (Germany); Taupitz, Matthias, E-mail: matthias.taupitz@harite.de [Department of Radiology, Charité – Universitätsmedizin Berlin, Charité Campus Mitte, Berlin (Germany); and others

    2014-04-15

    Purpose: To evaluate T1-relaxation times of chronic myocardial infarction (CMI) using gadobutrol and gadopentetate dimeglumine (Gd-DTPA) over time and to determine the optimal imaging window for late enhancement imaging with both contrast agents. Material and methods: Twelve patients with CMI were prospectively included and examined on a 1.5 T magnetic resonance (MR) system using relaxivity-adjusted doses of gadobutrol (0.15 mmol/kg) and Gd-DTPA (0.2 mmol/kg) in random order. T1-relaxation times of remote myocardium (RM), infarcted myocardium (IM), and left ventricular cavity (LVC) were assessed from short-axis TI scout imaging using the Look–Locker approach and compared intraindividually using a Wilcoxon paired signed-rank test (α < 0.05). Results: Within 3 min of contrast agent administration (CA), IM showed significantly lower T1-relaxation times than RM with both contrast agents, indicating beginning cardiac late enhancement. Differences between gadobutrol and Gd-DTPA in T1-relaxation times of IM and RM were statistically not significant through all time points. However, gadobutrol led to significantly higher T1-relaxation times of LVC than Gd-DTPA from 6 to 9 min (220 ± 15 ms vs. 195 ± 30 ms p < 0.01) onwards, resulting in a significantly greater ΔT1 of IM to LVC at 9–12 min (−20 ± 35 ms vs. 0 ± 35 ms, p < 0.05) and 12–15 min (−25 ± 45 ms vs. −10 ± 60 ms, p < 0.05). Using Gd-DTPA, comparable ΔT1 values were reached only after 25–35 min. Conclusion: This study indicates good delineation of IM to RM with both contrast agents as early as 3 min after administration. However, we found significant differences in T1 relaxation times with greater ΔT1 IM–LVC using 0.15 mmol/kg gadobutrol compared to 0.20 mmol/kg Gd-DTPA after 9–15 min post-CA suggesting earlier differentiability of IM and LVC using gadobutrol.

  5. New normal values not related to age and sex, of glomerular filtration rate by (99m)Tc-DTPA renal dynamic imaging, for the evaluation of living kidney graft donors.

    Science.gov (United States)

    Zhao, Xiuyi; Shao, Yahui; Wang, Yanming; Tian, Jun; Sun, Ben; Ru, Yanhui; Zhang, Aimin; Hao, Junwen

    2012-01-01

    The aim of this study was to investigate the normal values of glomerular filtration rate (GFR) by technetium-99m diaethylene-triamine-pentaacetic acid ((99m)Tc-DTPA) renal dynamic imaging for living kidney graft donors. In a total of 212 candidate donors, GFR was examined using (99m)Tc-DTPA renal dynamic imaging. Donors with GFR≥80mL/(min×1.73m(2)) and as low as with GFR≥70mL/(min×1.73m(2)) but a normal endogenous creatinine clearance rate (CCr) were quantified for living kidney donation. Differences in GFR levels based on sex and age were analyzed using rank correlation coefficient. Out of the 212 candidates, 161 were finally selected as kidney graft donors. The double kidney total GFR between the male and female donor groups, the GFR levels among differently-aged donor groups, and the GFR levels between the elderly (>55 years) and young- and middle-aged (≤55 years) donor groups did not show any significant difference (P>0.05). After kidney donation, renal function measured by blood urea nitrogen (BUN) and serum creatinine of all donors returned to normal within one week, and no serious complications were noticed. In conclusion, renal dynamic imaging by (99m)Tc-DTPA had a good accuracy and repeatability in GFR evaluation for living kidney donors. Candidate donors with GFR between 70mL/(min×1.73m(2)) and 80mL/(min×1.73m(2)) can be selected as kidney donors after strict screening. In living kidney donors GFR is not significantly correlated with age or sex.

  6. Noncommutative Yang-Mills-Higgs actions from derivation-based differential calculus

    CERN Document Server

    Cagnache, Eric; Wallet, Jean-Christophe

    2008-01-01

    Derivations of a (noncommutative) algebra can be used to construct various consistent differential calculi, the so-called derivation-based differential calculi. We apply this framework to the noncommutative Moyal algebras for which all the derivations are inner and analyse in detail the case where the derivation algebras generating the differential calculus are related to area preserving diffeomorphisms. The ordinary derivations corresponding to spatial dimensions are supplemented by additional derivations necessarely related to additional covariant coordinates. It is shown that these latter have a natural interpretation as Higgs fields when involved in gauge invariant actions built from the noncommutative curvature. The UV/IR mixing problem for (some of) the resulting Yang-Mills-Higgs models is discussed. A comparition to other noncommutative geometries already considered in the litterature is given.

  7. Metabolically Derived Human Ventilation Rates: A Revised Approach Based Upon Oxygen Consumption Rates (Final Report, 2009)

    Science.gov (United States)

    EPA announced the availability of the final report, Metabolically Derived Human Ventilation Rates: A Revised Approach Based Upon Oxygen Consumption Rates. This report provides a revised approach for calculating an individual's ventilation rate directly from their oxygen c...

  8. Gd-DTPA enhanced MR-follow-up of autologous patellar ligament transplants for anterior cruciate ligament repair. Gadolinium-DTPA gestuetzte MRT zur Beurteilung des Einheilens von autologen Ligamentum-patellae-Transplantaten als vorderer Kreuzbandersatzplastik

    Energy Technology Data Exchange (ETDEWEB)

    Tosch, U.; Felix, R. (Freie Univ. Berlin (Germany). Strahlenklinik und Poliklinik); Hertel, P.; Bernard, M. (Freie Univ. Berlin (Germany). Unfallchirurgische Abt. Martin-Luther Krankenhaus); Lais, E. (Freie Univ. Berlin (Germany). Unfallchirurgische Abt. Klinikum Rudolf Virchow)

    1993-01-01

    Eighteen patients with autologous patellar tendon transplant for reconstruction the anterior cruciate ligament were examined postoperatively. The time between operation and MR study was up to 3 weeks in ten patients, between 3 and 35 weeks in three patients and more than 35 weeks in five patients. In all patients, Gd-DTPA enhancement on the surface of the ligamentous transplant was visualized. This enhancement was between 1 and 10 mm thick. In all patients, complete stability of the knee joint was postoperatively. In 15 patients achieved flexion and extension were normal at the postoperative investigation. Three patients had a limitation of knee movement. In these MR studies, significant hyperplasia of the synovial membrane of the entire joint was diagnosed, explaining the persisting problems. Gd-DTPA-enhanced MR of the knee joint allows non-invasive documentation of remodelling following a patellar ligament transplant and possible complications. (orig.).

  9. The role of derivational paradigms with adjectival base in Old English word-formation

    Directory of Open Access Journals (Sweden)

    Carmen Novo Urraca

    2014-05-01

    Full Text Available The aim of this article is to identify the primary adjectives of Old English as well as to gather the derivational paradigms that revolve around them. All in all, 459 primary adjectives are identified, which function as base of 6,587 derivatives. Two conclusions are drawn from the analysis. In the first place, the concept of derivational paradigm contributes to the explanation of the overall organization of the lexicon, while allowing for the discussion of questions that are at the core of current morphological theory, such as recursivity and productivity. The conclusion is drawn that primary adjectives play a significant role in Old English derivation. Even though they are not as productive as strong verbs, primary adjectives function as base of derivation of a significant number of non-basic terms, which, moreover, belong to all lexical categories and nearly all grammatical classes.

  10. A NEW DERIVATIVE FREE OPTIMIZATION METHOD BASED ON CONIC INTERPOLATION MODEL

    Institute of Scientific and Technical Information of China (English)

    倪勤; 胡书华

    2004-01-01

    In this paper, a new derivative free trust region method is developed based on the conic interpolation model for the unconstrained optimization. The conic interpolation model is built by means of the quadratic model function, the collinear scaling formula, quadratic approximation and interpolation. All the parameters in this model are determined by objective function interpolation condition. A new derivative free method is developed based upon this model and the global convergence of this new method is proved without any information on gradient.

  11. Aqueous complexation of thorium(IV), uranium(IV), neptunium(IV), plutonium(III/IV), and cerium(III/IV) with DTPA.

    Science.gov (United States)

    Brown, M Alex; Paulenova, Alena; Gelis, Artem V

    2012-07-16

    Aqueous complexation of Th(IV), U(IV), Np(IV), Pu(III/IV), and Ce(III/IV) with DTPA was studied by potentiometry, absorption spectrophotometry, and cyclic voltammetry at 1 M ionic strength and 25 °C. The stability constants for the 1:1 complex of each trivalent and tetravalent metal were calculated. From the potentiometric data, we report stability constant values for Ce(III)DTPA, Ce(III)HDTPA, and Th(IV)DTPA of log β(101) = 20.01 ± 0.02, log β(111) = 22.0 ± 0.2, and log β(101) = 29.6 ± 1, respectively. From the absorption spectrophotometry data, we report stability constant values for U(IV)DTPA, Np(IV)DTPA, and Pu(IV)DTPA of log β(101) = 31.8 ± 0.1, 32.3 ± 0.1, and 33.67 ± 0.02, respectively. From the cyclic voltammetry data, we report stability constant values for Ce(IV) and Pu(III) of log β(101) = 34.04 ± 0.04 and 20.58 ± 0.04, respectively. The values obtained in this work are compared and discussed with respect to the ionic radius of each cationic metal.

  12. [Does intravenous gadolinium-DTPA administration have advantages in magnetic resonance imaging of acute injuries or chronic damage to the knee joint?].

    Science.gov (United States)

    Jerosch, J; Castro, W H; Müller, U; Assheuer, J

    1994-12-01

    79 patients with acute or chronic lesions of the knee were evaluated by MRI prior to and after application of Gd-DTPA. The MRI examination was performed by a 1.0 tesla imager with SE as well as FEDIF sequences. These MR studies were compared prior to and after intravenous Gd-DTPA application, focusing on the visibility and the definition of a possible lesion, and scored with a 3-point score. Statistic analysis and case analysis revealed that in patients with meniscus degeneration without a tear, Gd application yields no additional diagnostic information. However, in patients with meniscus tears Gd-DTPA significantly facilitates the definition of the lesion. Furthermore, Gd-DTPA makes differentiation possible between the synovial fluid and the synovial membrane. Whereas in cases with capsule or collateral ligament tears Gd-DTPA facilitates the documentation of the lesion, we found no advantage in using Gd-DPTA in patients with ACL tears. In patients with chondropathia patellae Gd-DTPA application supports the visualization of the secondary synovial reaction.

  13. Stability, protein binding and clearance studies of [99mTc]DTPA. Evaluation of a commercially available dry-kit

    DEFF Research Database (Denmark)

    Rehling, M

    1988-01-01

    [99mTc]DTPA has achieved widespread use for the measurement of glomerular filtration rate (GFR) with the single injection plasma clearance technique and for gamma-camera renography. However, the quality of the commercial preparations varies. The purpose of the present investigation was to study...... the quality of a commercial [99mTc]DTPA preparation (C.I.S., France) with reference to stability, protein binding and accuracy of the determined plasma clearance values as a measure of GFR. The stability of the preparations was studied by thin-layer chromatography, the in vitro protein binding by Sephadex...... filtration after incubation with human serum albumin and in vivo protein binding by filtration of human plasma. The accuracy of the plasma clearance values was investigated by comparison with the simultaneously measured plasma clearance of [51Cr]EDTA. There was no detectable free pertechnetate or hydrolysed...

  14. /sup 99m/Tc-DTPA renography before and after extracorporeal shock wave lithotripsy for renal calculi

    Energy Technology Data Exchange (ETDEWEB)

    Ohtake, Eiji; Murata, Hajime; Kanemura, Mikio; Yokoyama, Masao

    1988-09-01

    This study was undertaken to evaluate /sup 99m/Tc-DTPA renography before and after extracorporeal shock wave lithotripsy (ESWL). Twelve patients with renal calculi were examined in this study. In three patients, bilateral kidneys were treated with ESWL. Sequential renal images of the vascular phase, and the functional and excretory phases were taken using a gamma camera (ZLC 7500, Siemens), after intravenous injection of 555 MBq of /sup 99m/Tc-DTPA. Renograms were generated using data stored every 10 seconds for a period of 30 minutes by computer (Scintipac-2400, Shimadzu). Some treated kidneys were enlarged and/or showed uniform retention of radioactivity on sequential images, within a week after ESWL. Renogram patterns after ESWL were varied in each patient. The renogram findings after ESWL seemed to be affected by the presence or absence of stone fragments in the urinary tract.

  15. The fate of EDTA and DTPA in aquatic environments receiving waste water from two pulp and paper mills

    Energy Technology Data Exchange (ETDEWEB)

    Remberger, M.; Svenson, Anders

    1997-10-01

    To evaluate the fate of the complexing agents in receiving waters, two basic questions have been addressed: (i) are EDTA and DTPA found in the aquatic environment after discharge into receiving waters and (ii) are they photolytically converted. Two mills, one pulp mill localized at a fresh water lake and one pulp and paper mill at a brackish water were investigated, both mills using bleaching technologies with EDTA and DTPA as complexing agents. Samples were collected at the discharge point and along a gradient in the receiving waters at two occasions: summer at solstice and winter with low light intensity. Samples were taken from surface water, an intermediate depth, and bottom water. A new analytical method was applied, which made it possible to quantify the analytes at sub-{mu}g/l level. The complexing agents EDTA and DTPA and their primary degradation products were detected in the effluent and the receiving waters in the vicinity of the mills. DTPA and the degradation products could be detected a few kilometers from the effluent point while EDTA could be detected in more remote locations at fairly constant concentrations. The absorption of light in the sun spectrum in the water columns of the receiving waters was studied at different localities and during summer and winter conditions. The theoretical photochemical half-life of the ferric complex of EDTA in the surface layer of a central Swedish lake was confirmed. Analysis of EDTA in samples of receiving waters after photolytic treatment showed however, that a large portion of the complexing agent was unaffected by the treatment, indicating that most of the EDTA was complexed with other metals. EDTA in brackish water samples was unaffected by the photolytic treatment upon addition of excess ferric ions, except in winter close to the discharge point. The ease by which the ferric complexes are photochemically converted in ideal conditions seems to be hampered in receiving waters. 42 refs, 16 figs, 2 tabs

  16. Comparison of peritoneal equilibration test with {sup 99m}Tc-DTPA excretion in the assessment of peritoneal permeability

    Energy Technology Data Exchange (ETDEWEB)

    Das, B.K.; Senthilnathan, M.S.; Pradhan, P.K.; Nagabhushan, S. [Department of Nuclear Medicine, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Raebareli Road, 226014, Lucknow (India); Jeloka, T.K.; Sharma, R.K. [Department of Nephrology, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow (India)

    2004-05-01

    Assessment of peritoneal permeability is necessary for successful management of end-stage renal disease (ESRD) patients by continuous ambulatory peritoneal dialysis (CAPD). The objective of this study was to develop an alternative method of assessing the peritoneal permeability and to compare this method with the conventional method, the peritoneal equilibrium test, first described by Twardowski in 1987. Twenty patients undergoing regular CAPD were included in this study. Before starting the peritoneal dialysis, 370 MBq (10 mCi) technetium-99m diethylene triamine penta-acetic acid ({sup 99m}Tc-DTPA) was injected intravenously. A standard dose of the same quantity was kept and used later for calculations. At the end of 4 h, a dialysate fluid sample (1 ml) was collected and the total dialysis effluent fluid volume was measured. Excretion of {sup 99m}Tc-DTPA into the dialysate fluid as a percentage of the injected dose was calculated. Simultaneously, standard peritoneal equilibrium test values were recorded for comparison. Peritoneal excretion of {sup 99m}Tc-DTPA ranged from 8% to 25% of the injected dose, depending on the peritoneal membrane permeability. When the results were compared with the conventional method, a good correlation (r=0.79) was found. This innovative radionuclide technique is a simple and convenient method to assess the peritoneal membrane permeability and can be used as an alternative to the peritoneal equilibrium test, which is very cumbersome and associated with many limitations. (orig.)

  17. MR liver imaging with Gd-EOB-DTPA: a delay time of 10 minutes is sufficient for lesion characterisation

    Energy Technology Data Exchange (ETDEWEB)

    Kessel, C.S. van [University Medical Centre Utrecht, Department of Radiology, Utrecht (Netherlands); University Medical Centre Utrecht, Department of Surgery, Utrecht (Netherlands); Veldhuis, W.B.; Bosch, M.A.A.J. van den; Leeuwen, M.S. van [University Medical Centre Utrecht, Department of Radiology, Utrecht (Netherlands)

    2012-10-15

    To assess whether, in patients with normal liver function, a hepatobiliary delay time of 10 min after Gd-EOB-DTPA injection is sufficient for lesion characterisation. In 42 consecutive patients with suspected focal liver lesions, dynamic MRI was performed after intravenous Gd-EOB-DTPA, followed by hepatobiliary phases at 5, 10 and 20 min. The following items were assessed at each hepatobiliary phase: parenchymal enhancement, contrast agent excretion in bile ducts, lesion enhancement characteristics (hypo-, iso-, or hyperintensity, rim enhancement, central non-enhancement), and contrast- and signal-to-noise ratios, separately for hypo- and hyperintense lesions. Following enhancement, parenchymal signal intensity increased significantly up to 10 min (86.3%, P < 0.001), and subsequently stabilised (86.5% after 20 min, P = 0.223). Biliary contrast agent excretion was first observed in 2, 32 and 5 patients after 5, 10 and 20 min respectively. Hepatobiliary lesion enhancement characteristics observed after 5 min persisted during later hepatobiliary phases. CNR and SNR ratios increased significantly (P < 0.05) up to 10 min after enhancement without further increase at 20 min, in hypo- and hyperintense lesions. If lesion characterisation is the primary reason for performing MRI, a hepatobiliary delay time of 10 min after Gd-EOB-DTPA injection is sufficient in patients with normal liver function. (orig.)

  18. Efeito da reação do solo no zinco extraído pelas soluções de dtpa e Mehlich-1 Soil reaction effect on dtpa and Mehlich-1 extractable zinc

    Directory of Open Access Journals (Sweden)

    Cleide Aparecida de Abreu

    1996-01-01

    Full Text Available A análise do solo é uma ferramenta eficiente para avaliar a disponibilidade de Zn, desde que seja escolhido adequadamente o processo de extração. Neste trabalho, desenvolvido em casa de vegetação, em Campinas, em 1993, avaliou-se a influência da calagem no Zn extraível do solo, usando-se o milho como planta-teste. Utilizaram-se amostras de cinco solos, sendo duas de latossolo vermelho-amarelo, uma de latossolo húmico, uma de latossolo roxo e uma de terra roxa estruturada, as quais receberam cinco doses de calcário. Antes e depois do cultivo do milho, retiraram-se amostras de solos para análise de Zn, usando-se os extratores DTPA e Mehlich-1. Na fase inicial, as plantas de milho apresentaram sintomas de deficiência de Zn, caracterizados por uma coloração amarelo-clara e altura reduzida. 0 extrator DTPA mostrou os melhores resultados com valores altos e positivos de correlação entre os teores de Zn na planta e no solo. Concluiu-se que a mudança na disponibilidade de Zn, decorrente da reação do solo, foi mais bem avaliada pela solução de DTPA.Soil testing can be adequate to evaluate the Zn availability if a good method is chosen. This experiment was conducted in a greenhouse, in Campinas, State of São Paulo, Brazil, in 1993, to evaluate the influence of liming on extractable Zn from soils, using corn as a test plant. Five rates of limestone were applied to four Oxisols and one Alfisol. Before and after the cultivation of corn, soil samples were taken and analyzed by extracting procedures of DTPA and Mehlich-1. Five days after emergence, corn seedlings showed leaf yellowing and reduced plant height (typical Zn deficiency symptoms, variable according to the liming level and soil studied. DTPA methodology showed the best results, with high and positive correlations estimated between plant-Zn and soil-Zn contents.

  19. Preparation of 99mTc-Gd-DTPA-BMA and its bio-distribution in nude mice bearing tumor%99mTc-Gd-DTPA-BMA的制备及其在荷瘤裸鼠体内的生物学分布

    Institute of Scientific and Technical Information of China (English)

    曹丰; 陈跃; 黄占文; 丁志凌; 张伟

    2011-01-01

    目的 探讨以99mTc标记Gd-DTPA-BMA的可行性及其在荷瘤裸鼠体内分布的特征.方法 采用氯化亚锡还原法,用99mTc标记Gd-DTPA-BMA,TLC法测定99mTc-Gd-DTPA-BMA的放射化学纯度,将标记物注入荷A549肿瘤裸鼠体内,分别行SPECT显像及MRI增强成像,并测定其体内分布.结果 99mTc-Gd-DTPA-BMA放射化学纯度为99.2%,室温放置6 h后放射化学纯度大于95%.该标记物主要通过肾脏排泄,注射后肿瘤组织在SPECT及MRI下均能获得较好的显示或增强,且在肿瘤组织中具有较高的每克组织百分注射剂量率.结论 作为一种潜在的双模式显像剂,99mTc-Gd-DTPA-BMA值得进一步研究.%Objective To explore the feasibility of using 99mTc labeled Gd-DTPA-BMA, and to observe its characteristics of distribution in nude mice bearing tumor. Methods The labeled compounds were prepared by reducing 99mTc in the presence of Gd-DTPA-BMA with stannous chloride (SnCl2). The radiochemical purity was determined by TLC. 99mTc-Gd-DTPA-BMA was injected into nude mice bearing A549 tumor. SPECT and MR imaging were performed, respectively. The in vivo bio-distribution in nude mice was observed. Results The radiochemical purity of 99mTc-Gd-DTPA-BMA was 99.2%,and remained more than 95 % within 6 h at room temperature. 99mTc-Gd-DTPA-BMA imaging and bio-distribution experiments showed that the marker was excreted by renal. After inject 99mTc-Gd-DTPA-BMA, SPECT and MRI showed the tumor tissue or enhancement clearly, and high percentage of the injected dose per gram of tissues mass was noticed. Conclusion As a potential dual-modal imaging agent, 99mTc-Gd-DTPA-BMA is worthy of further research.

  20. Separation of piracetam derivatives on polysaccharide-based chiral stationary phases.

    Science.gov (United States)

    Kažoka, H; Koliškina, O; Veinberg, G; Vorona, M

    2013-03-15

    High-performance liquid chromatography was used for the enantiomeric separation of two chiral piracetam derivatives. The suitability of six commercially available polysaccharide-based chiral stationary phases (CSPs) under normal phase mode for direct enantioseparation has been investigated. The influence of the CSPs as well the nature and content of an alcoholic modifier in the mobile phase on separation and elution order was studied. It was established that CSP Lux Amylose-2 shows high chiral recognition ability towards 4-phenylsubstituted piracetam derivatives.

  1. MR imaging of the biliary tract with Gd-EOB-DTPA: Effect of liver function on signal intensity

    Energy Technology Data Exchange (ETDEWEB)

    Takao, Hidemasa, E-mail: takaoh-tky@umin.ac.jp [Department of Radiology, Graduate School of Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655 (Japan); Akai, Hiroyuki; Tajima, Taku [Department of Radiology, Graduate School of Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655 (Japan); Kiryu, Shigeru [Department of Radiology, Institute of Medical Science, University of Tokyo, 74-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639 (Japan); Watanabe, Yasushi [Department of Radiological Technology, The University of Tokyo Hospital, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655 (Japan); Imamura, Hiroshi [Department of Hepatobiliary-Pancreatic Surgery, Juntendo University School of Medicine, 3-1-3 Hongo, Bunkyo-ku, Tokyo 113-8431 (Japan); Akahane, Masaaki; Yoshioka, Naoki [Department of Radiology, Graduate School of Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655 (Japan); Kokudo, Norihiro [Hepato-Biliary-Pancreatic Surgery Division, Department of Surgery, Graduate School of Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655 (Japan); Ohtomo, Kuni [Department of Radiology, Graduate School of Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655 (Japan)

    2011-02-15

    Objective: To quantitatively evaluate the signal intensity of the biliary tract in gadolinium ethoxybenzyl diethylenetriamine pentaacetic acid (Gd-EOB-DTPA)-enhanced magnetic resonance (MR) imaging and to investigate the effect of liver function on the signal intensity of the biliary tract. Materials and methods: A total of 32 patients with and without chronic liver disease (normal liver group, n = 15; chronic liver disease group, n = 17) were included in this study. All patients were prospectively enrolled for evaluation of known or suspected focal liver lesions. In the chronic liver disease group, the etiologies were chronic hepatitis C virus infection (n = 12) and chronic hepatitis B virus infection (n = 5). The median Child-Pugh score was 5 (range, 5-7). Each patient received the standard dose of Gd-EOB-DTPA (0.025 mmol/kg of body weight). Post-contrast T1-weighted MR images were obtained at 5, 10, 15, 20, 25, and 30 min after administration of Gd-EOB-DTPA. Maximum signal intensities (SIs) of the right and left hepatic ducts, common hepatic duct, and common bile duct were measured. Relative signal intensity was calculated as follows: relative SI = maximum SI{sub bileduct}/mean SI{sub muscle}. Serum albumin level, serum total bilirubin level, prothrombin time, indocyanine green retention rate at 15 min (ICG-R15), and estimated glomerular filtration rate were entered into regression analysis. Results: The signal intensity of the bile duct reached a peak 30 min after administration of Gd-EOB-DTPA. The mean relative signal intensity of the right and left hepatic ducts at the peak time point was not significantly different between the two groups, while increase in signal intensity was delayed in the chronic liver disease group. The mean relative signal intensity of the common hepatic duct and that of the common bile duct at the peak time point were significantly different between the two groups (Wilcoxon rank-sum test, P = 0.03, respectively). Stepwise regression

  2. Preparation and quality control of {sup 166}Ho-DTPA-antiCD20 for radioimmunotherapy

    Energy Technology Data Exchange (ETDEWEB)

    Zolghadri, S.; Jalilian, A.R.; Yousefnia, H.; Bahrami-Sumani, A.; Shirvani-Arani, S.; Ghannadi-Maragheh, M. [Nuclear Science and Technology Research Institute (NSTRI), Tehran (IR). Radiopharmaceutical Research and Development Lab. (RRDL)

    2011-07-01

    In this work, anti-CD20 was successively labeled with beta-particle emitting radionuclide, Ho-166, for ultimate radioimmunotherapy applications. Ho-166 chloride was obtained by thermal neutron flux (1 x 10{sup 13} n cm{sup -2} s{sup -1}) of natural Ho{sub 2}(NO{sub 3}){sub 3} sample, dissolved in acidic media. {sup 166}Ho-holmium chloride (185 MBq) was added to the conjugated antibody after ccDTPA residulation at room temperature. Radiochemical purity of 95% (ITLC) and 98% (HPLC) were obtained for final radioimmunoconjugate (specific activity = 3-3.5 GBq/mg). The final isotonic {sup 166}Ho-rituximab complex was checked by gel electrophoresis for protein integrity retention. Biodistribution studies of Ho-166 chloride and radioimmunoconjugate were performed in wild-type rats to determine the biodistribution. The accumulation of the radiolabeled antibody in lungs, liver and spleen demonstrates a similar pattern to the other radiolabeled anti-CD20 immunoconjugates. (orig.)

  3. Gd-EOB-DTPA-enhanced magnetic resonance imaging features of hepatic hemangioma compared with enhanced computed tomography

    Institute of Scientific and Technical Information of China (English)

    Akihiro Tateyama; Yoshihiko Fukukura; Koji Takumi; Toshikazu Shindo; Yuichi Kumagae; Kiyohisa Kamimura; Masayuki Nakajo

    2012-01-01

    AIM:To clarify features of hepatic hemangiomas on gadolinium-ethoxybenzyl-diethylenetriaminpentaacetic acid (Gd-EOB-DTPA)-enhanced magnetic resonance imaging (MRI) compared with enhanced computed tomography (CT).METHODS:Twenty-six patients with 61 hepatic hemangiomas who underwent both Gd-EOB-DTPA-enhanced MRI and enhanced CT were retrospectively reviewed.Hemangioma appearances (presence of peripheral nodular enhancement,central nodular enhancement,diffuse homogenous enhancement,and arterioportal shunt during the arterial phase,fill-in enhancement during the portal venous phase,and prolonged enhancement during the equilibrium phase) on Gd-EOB-DTPA-enhanced MRI and enhanced CT were evaluated.The degree of contrast enhancement at the enhancing portion within the hemangioma was visually assessed using a five-point scale during each phase.For quantitative analysis,the tumor-muscle signal intensity ratio (SIR),the liver-muscle SIR,and the attenuation value of the tumor and liver parenchyma were calculated.The McNemar test and the Wilcoxon's signed rank test were used to assess the significance of differences in the appearances of hemangiomas and in the visual grade of tumor contrast enhancement between Gd-EOB-DTPA-enhanced MRI and enhanced CT.RESULTS:There was no significant difference between Gd-EOB-DTPA-enhanced MRI and enhanced CT in the presence of peripheral nodular enhancement (85%vs 82%),central nodular enhancement (3% vs 3%),diffuse enhancement (11% vs 16%),or arterioportal shunt (23% vs 34%) during arterial phase,or fill-in enhancement (79% vs 80%) during portal venous phase.Prolonged enhancement during equilibrium phase was observed less frequently on Gd-EOB-DTPA-enhanced MRI than on enhanced CT (52% vs 100%,P < 0.001).On visual inspection,there was significantly less contrast enhancement of the enhancing portion on GdEOB-DTPA-enhanced MRI than on enhanced CT during the arterial (3.94 ± 0.98 vs 4.57 ± 0.64,respectively,P < 0

  4. C60-Based Ebselen Derivative: Synthesis and Enhanced Protective Effect on Mouse Thymus Cells

    Institute of Scientific and Technical Information of China (English)

    LIU,Xu-Feng; GUAN,Wen-Chao; KE,Wen-Shan

    2008-01-01

    A C60-based ebselen derivative 3 was synthesized through a Bingel cyclopropanation of C60 with the ebselen malonate 2. It was obtained in a three-step synthesis starting from 2-(chloroseleno)benzoyl chloride and 2-(2-aminoethoxy)ethanol, in a 42% yield (based on consumed C60). Its structure was characterized by 1H NMR,13C NMR, IR, FAB-MS, and elemental analyses techniques. To verify that the C60-based ebselen derivative 3 had enhanced effect on viability of mouse thymus cells, the C60 derivative 4 and ebselen derivative 2 were selected to treat the mouse thymus cells using the same procedures as those with the C60-based ebselen derivative 3. The result shows that MTT(OD) values of compound 3 treated groups (0.335 ±0.021) were all higher than those of compound 4 (0.283±0.031) and compound 2 (0.247±0.025) treated groups, indicating that the compound 3 has an advantage over compounds 2 and 4 in promoting the viability of the mouse thymus cell.

  5. Pulmonary functional MRI:an animal model study of oxygen-enhanced ventilation combined with Gd-DTPA-enhanced perfusion

    Institute of Scientific and Technical Information of China (English)

    杨健; 万明习; 郭佑民

    2004-01-01

    Background The assessment of regional pulmonary ventilation and perfusion is essential for the evaluation of a variety of lung disorders. Pulmonary ventilation MRI using inhaled oxygen as a contrast medium can be obtained with a clinical MR scanner, without additional equipment, and has been demonstrated to be a feasible means of assessing ventilation in animal models and some clinical patients. However, few studies have reported on MR ventilation-perfusion imaging. In this study, we evaluated the usefulness of oxygen-enhanced ventilation in combination with first-pass Gd-DTPA-enhanced perfusion MRI in a canine model of pulmonary embolism and airway obstruction.Methods Peripheral pulmonary embolisms were produced in eight dogs by intravenous injection of gelfoam strips at the pulmonary segmental arterial level, and airway obstructions were created in five of the dogs by inserting a self-designed balloon catheter into a secondary bronchus. Oxygen-enhanced MR ventilation images were produced by subtracting images from before and after inhalation of pure oxygen. Pulmonary perfusion MR images were acquired with a dynamic three-dimensional fast gradient-echo sequence. MR ventilation and perfusion images were read and contrasted with results from general examinations of pathological anatomy, ventilation-perfusion scintigraphy, and pulmonary angiography. Results Regions identified as having airway obstructions matched using both MR ventilation and perfusion imaging, but regions of pulmonary embolisms were mismatched. The area of airway obstruction defects was smaller using MR ventilation imagery than that using ventilation scintigraphy. Abnormal perfusion regions due to pulmonary embolisms were divided into defective regions and reduced regions based on the time course of signal intensity changes. In the diagnosis of pulmonary embolisms with the technique of ventilation and perfusion MRI, sensitivity and specificity were 75.0% and 98.1%, respectively, and the diagnostic

  6. Synthesis and characterization of nickel complexes derived from Schiff-Bases

    OpenAIRE

    Mirian Y. Matsumoto; Keila B. Dias; Vicente L. Kupfer; Elias B. O. Filho; Larisse G. Salazar; Adalberto V. Mezacasa; Persiely P. Rosa; Nelson Luis de Campos Domingues; Andrelson W. Rinaldi

    2012-01-01

    Aromatic Schiff-Bases constitute a large class of organic compounds containing the imine group (C=N) [1-3]. 2-hydroxybenzaldehyde (salicylaldehyde) which has been frequently used for the synthesis of aromatic Schiff-Bases, it has many biological applications, such as analgesics, anti-inflammatory, antibacterial, antiviral and bactericidal activities [4-6]. From above, this project proposed the synthesis of three Schiff bases derived from salicylaldehyde prepared by condensation between salicy...

  7. CFD Based Determination of Dynamic Stability Derivatives in Yaw for a Bird

    Institute of Scientific and Technical Information of China (English)

    M. A. Moelyadi; G. Sachs

    2007-01-01

    Dynamic yaw stability derivatives of a gull bird are determined using Computational Fluid Dynamics(CFD) method. Two kinds of motions are applied for calculating the dynamic yaw stability derivatives CNr and CNβ. The first one relates to a lateral translation and, separately, to a yaw rotation. The second one consists of a combined translational and rotational motion. To determine dynamic yaw stability derivatives, the simulation of an unsteady flow with a bird model showing a harmonic motion is performed. The flow solution for each time step is obtained by solving unsteady Euler equations based on a finite volume approach for a small reduced frequency. Then, an evaluation of unsteady forces and moments for one cycle is conducted using harmonic Fourier analysis. The results of the dynamic yaw stability derivatives for both simulations of the model show a good agreement.

  8. Carbon-Based Functional Materials Derived from Waste for Water Remediation and Energy Storage.

    Science.gov (United States)

    Ma, Qinglang; Yu, Yifu; Sindoro, Melinda; Fane, Anthony G; Wang, Rong; Zhang, Hua

    2017-01-23

    Carbon-based functional materials hold the key for solving global challenges in the areas of water scarcity and the energy crisis. Although carbon nanotubes (CNTs) and graphene have shown promising results in various fields of application, their high preparation cost and low production yield still dramatically hinder their wide practical applications. Therefore, there is an urgent call for preparing carbon-based functional materials from low-cost, abundant, and sustainable sources. Recent innovative strategies have been developed to convert various waste materials into valuable carbon-based functional materials. These waste-derived carbon-based functional materials have shown great potential in many applications, especially as sorbents for water remediation and electrodes for energy storage. Here, the research progress in the preparation of waste-derived carbon-based functional materials is summarized, along with their applications in water remediation and energy storage; challenges and future research directions in this emerging research field are also discussed.

  9. Sigmoid function based integral-derivative observer and application to autopilot design

    Science.gov (United States)

    Shao, Xingling; Wang, Honglun; Liu, Jun; Tang, Jun; Li, Jie; Zhang, Xiaoming; Shen, Chong

    2017-02-01

    To handle problems of accurate signal reconstruction and controller implementation with integral and derivative components in the presence of noisy measurement, motivated by the design principle of sigmoid function based tracking differentiator and nonlinear continuous integral-derivative observer, a novel integral-derivative observer (SIDO) using sigmoid function is developed. The key merit of the proposed SIDO is that it can simultaneously provide continuous integral and differential estimates with almost no drift phenomena and chattering effect, as well as acceptable noise-tolerance performance from output measurement, and the stability is established based on exponential stability and singular perturbation theory. In addition, the effectiveness of SIDO in suppressing drift phenomena and high frequency noises is firstly revealed using describing function and confirmed through simulation comparisons. Finally, the theoretical results on SIDO are demonstrated with application to autopilot design: 1) the integral and tracking estimates are extracted from the sensed pitch angular rate contaminated by nonwhite noises in feedback loop, 2) the PID(proportional-integral-derivative) based attitude controller is realized by adopting the error estimates offered by SIDO instead of using the ideal integral and derivative operator to achieve satisfactory tracking performance under control constraint.

  10. A kit to prepare {sup 111}In-DTPA-trastuzumab (Herceptin) Fab fragments injection under GMP conditions for imaging or radioimmunoguided surgery of HER2-positive breast cancer

    Energy Technology Data Exchange (ETDEWEB)

    Scollard, Deborah A.; Chan, Conrad [Department of Pharmaceutical Sciences, University of Toronto, Toronto, ON, M5S 3M2 (Canada); Holloway, Claire M.B. [Department of Surgery, Sunnybrook Health Sciences Centre, Toronto, ON, M4N 1H1 (Canada); Reilly, Raymond M., E-mail: raymond.reilly@utoronto.c [Department of Pharmaceutical Sciences, University of Toronto, Toronto, ON, M5S 3M2 (Canada); Department of Medical Imaging, University of Toronto, Toronto, ON, M5S 3E2 (Canada); Toronto General Research Institute, University Health Network, Toronto, ON, M5G 2M9 (Canada)

    2011-01-15

    Introduction: The human epidermal growth factor receptor-2 (HER2) gene is amplified in 25% of invasive breast cancers, and receptor overexpression has been noted in up to 60% of early stages of the disease [ductal carcinoma in situ (DCIS)]. Preclinical studies have revealed high tumor/blood ratios (>27:1) for {sup 111}In-labeled Fab fragments of the HER2 monoclonal antibody, trastuzumab (Herceptin) ({sup 111}In-DTPA-trastuzumab Fab) at 72 h pi in athymic mice bearing subcutaneous human breast cancer xenografts. Our aim in this study was to formulate a kit for preparation of {sup 111}In-DTPA-trastuzumab Fab injection under good manufacturing practice (GMP) conditions suitable for human administration in a Phase I clinical trial of imaging and radioimmunoguided surgery (RIGS) of HER2-positive breast cancer. Methods: Fab fragments were produced by digestion of trastuzumab IgG (Herceptin) with immobilized papain for 20 h at 37{sup o}C. Fab fragments were purified by ultrafiltration, then reacted with a 10-fold molar excess of diethylenetriaminepentaacetic acid (DTPA) dianhydride. DTPA-Fab fragments were purified, then sterilized by filtration into unit dose glass vials (kits). Kits were tested against specifications for volume (0.9-1.1 ml), protein concentration (0.45-0.55 mg/ml), pH (5.5-6.5), DTPA substitution (0.5-4.0 mol DTPA/mol Fab), appearance (clear, colorless and particle free), labeling efficiency ({>=}85%), and sterility and apyrogenicity (USP XXXII). Immunoreactivity of {sup 111}In-DTPA-trastuzumab Fab towards HER2 was measured by saturation radioligand binding assays using SKBR-3 human breast cancer cells (specifications: K{sub a}=0.6-9.6x10{sup 7} L/mol; B{sub max}=0.6-10.4x10{sup 6} sites/cell). {sup 111}In-DTPA-trastuzumab Fab injection was prepared by adding 80-100 MBq of {sup 111}InCl{sub 3} to a single kit vial and incubating for 30 min at room temperature. {sup 111}In-DTPA-trastuzumab Fab was assayed for the amount of radioactivity and tested for p

  11. Analytical derivation: An epistemic game for solving mathematically based physics problems

    Science.gov (United States)

    Bajracharya, Rabindra R.; Thompson, John R.

    2016-06-01

    Problem solving, which often involves multiple steps, is an integral part of physics learning and teaching. Using the perspective of the epistemic game, we documented a specific game that is commonly pursued by students while solving mathematically based physics problems: the analytical derivation game. This game involves deriving an equation through symbolic manipulations and routine mathematical operations, usually without any physical interpretation of the processes. This game often creates cognitive obstacles in students, preventing them from using alternative resources or better approaches during problem solving. We conducted hour-long, semi-structured, individual interviews with fourteen introductory physics students. Students were asked to solve four "pseudophysics" problems containing algebraic and graphical representations. The problems required the application of the fundamental theorem of calculus (FTC), which is one of the most frequently used mathematical concepts in physics problem solving. We show that the analytical derivation game is necessary, but not sufficient, to solve mathematically based physics problems, specifically those involving graphical representations.

  12. Usefulness of {sup 99m}Tc-DTPA renography and diuretic renography in predicting successful stone discharge following outpatient ESWL in patients with a single ureteral stone

    Energy Technology Data Exchange (ETDEWEB)

    Soga, Norihito; Komeda, Yoshinori [Yokkaichi Health Insurance Hospital, Mie (Japan); Suzuki, Ryuichi; Kawamura, Juichi

    1996-11-01

    We analyzed the {sup 99m}Tc-DTPA renogram with and without diuresis to predict the possibility of stone discharge on the outpatient basis by renogram patterns. Between October 1993 and December 1995, {sup 99m}Tc-DTPA renography was performed in 79 patients with a single ureteral stone. The {sup 99m}Tc-DTPA renogram pattern was classified into the three types of normal function, obstruction and lower function patterns and the complete stone discharge rate was 93, 63 and 25%, respectively. In addition, diuretic renography using Furosemide was performed in patients with an obstruction pattern and the three renogram patterns of return to the normal curve, a diuretic response and no response were obtained; the complete stone discharge rate was 44, 65.3 and 93%, respectively. From this study, patients with a single ureteral stone with a normal pattern on the regular DTPA renogram and patients with no response pattern on the diuretic renogram, even if in such patients an obstructive pattern was seen on the regular DTPA renogram, seem to be a good candidate for obtaining a high rate of a stone discharge with extracorporeal shock wave lithotripsy (ESWL) treatment in the outpatients basis. (author)

  13. Detection of active bile leak with Gd-EOB-DTPA enhanced MR cholangiography: Comparison of 20–25 min delayed and 60–180 min delayed images

    Energy Technology Data Exchange (ETDEWEB)

    Cieszanowski, Andrzej, E-mail: andrzej.cieszanowski@wum.edu.pl [2nd Department of Clinical Radiology, Medical University of Warsaw, ul. Banacha 1A, 02-097 Warszawa (Poland); Stadnik, Anna, E-mail: aniaws@yahoo.com [2nd Department of Clinical Radiology, Medical University of Warsaw, ul. Banacha 1A, 02-097 Warszawa (Poland); Lezak, Aleksandra, E-mail: aleksandralezak@gmail.com [2nd Department of Clinical Radiology, Medical University of Warsaw, ul. Banacha 1A, 02-097 Warszawa (Poland); Maj, Edyta, E-mail: em26@wp.pl [2nd Department of Clinical Radiology, Medical University of Warsaw, ul. Banacha 1A, 02-097 Warszawa (Poland); Zieniewicz, Krzysztof, E-mail: krzysztof.zieniewicz@wum.edu.pl [Chair and Department of General, Transplant and Liver Surgery, Medical University of Warsaw, ul. Banacha 1A, 02-097 Warszawa (Poland); Rowinska-Berman, Katarzyna, E-mail: kasiarowinska@wp.pl [2nd Department of Clinical Radiology, Medical University of Warsaw, ul. Banacha 1A, 02-097 Warszawa (Poland); Grudzinski, Ireneusz P., E-mail: ireneusz.grudzinski@wum.edu.pl [Department of Toxicology, Medical University of Warsaw, Faculty of Pharmacy, ul. Banacha 1, 02-097 Warsaw (Poland); Krawczyk, Marek, E-mail: marek.krawczyk@wum.edu.pl [Chair and Department of General, Transplant and Liver Surgery, Medical University of Warsaw, ul. Banacha 1A, 02-097 Warszawa (Poland); Rowiński, Olgierd, E-mail: olgierd.rowinski@wum.edu.pl [2nd Department of Clinical Radiology, Medical University of Warsaw, ul. Banacha 1A, 02-097 Warszawa (Poland)

    2013-12-01

    Objectives: The purpose of this study was to assess the value of contrast-enhanced magnetic resonance cholangiography (MRC) performed in different time delays after injection of gadoxetic acid disodium (Gd-EOB-DTPA) for the diagnosis of active bile leak. Methods: This retrospective analysis included Gd-EOB-DTPA enhanced MR images of 34 patients suspected of bile leak. Images were acquired 20–25 min after Gd-EOB-DTPA injection. If there was inadequate contrast in the bile ducts then delayed images after 60–90 min and 150–180 min were obtained. Results were correlated with intraoperative findings, ERCP results, clinical data, laboratory tests, and follow-up examinations. Results: Gd-EOB-DTPA enhanced MRC yielded an overall sensitivity of 96.4%, specificity of 100% and accuracy of 97.1% for the diagnosis of an active bile leak. The sensitivity of 20–25 min delayed MR images was 42.9%, of combined 20–25 min and 60–90 min delayed images was 92.9% and of combined 20–25 min, 60–90 min and 150–180 min delayed images was 96.4%. Conclusions: Gd-EOB-DTPA enhanced MRC utilizing delayed phase images was effective for detecting the presence and location of active bile leaks. The images acquired 60–180 min post-injection enabled identification of bile leaks even in patients with a dilated biliary system or moderate liver dysfunction.

  14. Diagnosis und differential diagnosis of focal liver lesions using the hepatocyte-specific contrast agent Gd-EOB-DTPA; Diagnose und Differenzialdiagnose fokaler Leberlaesionen unter Verwendung von Gd-EOB-DTPA (Primovist)

    Energy Technology Data Exchange (ETDEWEB)

    Ringe, Kristina Imeen [Medizinische Hochschule Hannover (Germany). Inst. fuer Diagnostische und Interventionelle Radiologie

    2016-03-15

    The implementation of MR contrast agents significantly improves detection and characterization of focal liver lesions. This article is focusing on the hepatocyte-specific contrast agent Gd-EOB-DTPA, which has been available in Germany for liver MRI since 2004. In the introduction, the specific pharmacokinetic and -dynamic properties of this contrast agent are reviewed, and a workflow-optimized pulse sequence protocol for comprehensive hepatobiliary MRI in daily routine is presented. Afterwards, the appearance of the most common focal liver lesions is illustrated, including the differences to the use of extracellular contrast agents as well as a discussion of the most important differential diagnoses.

  15. First experiences with application of gadolinium-DTPA in infants and small children under two years of age. Erste Erfahrungen beim Einsatz von Gadolinium-DTPA bei Saeuglingen und Kleinkindern unter zwei Jahren

    Energy Technology Data Exchange (ETDEWEB)

    Maas, R.; Lausch, N.; Buecheler, E. (Hamburg Univ. (Germany). Radiologische Klinik); Grzyska, U. (Hamburg Univ. (Germany). Neurologische Klinik)

    1991-07-01

    The use of Gadolinium-DTPA as a paramagnetic contrast agent in MRI with adults and juveniles concerning brain and spinal cord pathology is well proven since years. In the FRG it is only introduced for children over two years of age. Therefore this report deales with the experience in four infants and small children under the age of two. They all were suffering from neurological tumors and got additional diagnostic information in three cases. Clinical side-effects like urticaria, vomiting or convulsions had not been observed nor had pathologic changes of the biochemical parameters been noticed. (orig./GDG).

  16. Synthesis and antitumor evaluation of arctigenin derivatives based on antiausterity strategy.

    Science.gov (United States)

    Kudou, Naoki; Taniguchi, Akira; Sugimoto, Kenji; Matsuya, Yuji; Kawasaki, Masashi; Toyooka, Naoki; Miyoshi, Chika; Awale, Suresh; Dibwe, Dya Fita; Esumi, Hiroyasu; Kadota, Shigetoshi; Tezuka, Yasuhiro

    2013-02-01

    A series of new (-)-arctigenin derivatives with variably modified O-alkyl groups were synthesized and their preferential cytotoxicity was evaluated against human pancreatic cancer cell line PANC-1 under nutrient-deprived conditions. The results showed that monoethoxy derivative 4i (PC(50), 0.49 μM), diethoxy derivative 4h (PC(50), 0.66 μM), and triethoxy derivative 4m (PC(50), 0.78 μM) showed the preferential cytotoxicities under nutrient-deprived conditions, which were identical to or more potent than (-)-arctigenin (1) (PC(50), 0.80 μM). Among them, we selected the triethoxy derivative 4m and examined its in vivo antitumor activity using a mouse xenograft model. Triethoxy derivative 4m exhibited also in vivo antitumor activity with the potency identical to or slightly more than (-)-arctigenin (1). These results would suggest that a modification of (-)-arctigenin structure could lead to a new drug based on the antiausterity strategy.

  17. Evaluation of Ferrocene Derivatives as Burn Rate Modifiers in AP/HTPB-Based Composite Propellants

    Directory of Open Access Journals (Sweden)

    G. M. Gore

    1999-04-01

    Full Text Available Some ferrocene derivatives like 2,4-dinitrophenylhydrazine derivative of acetyl ferrocene, 1-pyrrolidinylmethyl ferrocene, di-ter-butyl ferrocene and 1,3-diferrocenyl-l-butene (DFB have been synthesised and characterised by infrared, nuclear magnetic resonance, ultraviolet, iron content, etc. To study the effect of their incorporation on performance, ammonium perchlorate/hydroxyl-terminated polybutadiene-based composite propellants containing these derivatives have been prepared and studied for burn rates, tensile strength and percentage elongation followed by their static test evaluation. A comparison of the properties of propellant containing solid and liquid ferrocene derivatives has been made with those containing Fe/sub 2/O/sub 3/ and n-butyl ferrocene, respectively. The data clearly indicates that these ferrocene derivatives are better than Fe/sub 2/O/sub 3/ and n-butyl errocene. Also, DFB is the best among these derivatives. Like composite propellants, DFB increases burn rate in fuel-rich propellants also.

  18. Single-phase Phase-locked Loop Based on Derivative Elements

    DEFF Research Database (Denmark)

    Guan, Qingxin; Zhang, Yu; Kang, Yong;

    2017-01-01

    High performance phase locked loops (PLLs) are critical for power control in grid-connected systems. This paper presents a new method of designing a PLL for single-phase systems based on derivative elements (DEs). The quadrature signal generator (QSG) is constructed by two DEs with the same main ...

  19. CONFAC Decomposition Approach to Blind Identification of Underdetermined Mixtures Based on Generating Function Derivatives

    NARCIS (Netherlands)

    de Almeida, Andre L. F.; Luciani, Xavier; Stegeman, Alwin; Comon, Pierre

    2012-01-01

    This work proposes a new tensor-based approach to solve the problem of blind identification of underdetermined mixtures of complex-valued sources exploiting the cumulant generating function (CGF) of the observations. We show that a collection of second-order derivatives of the CGF of the observation

  20. Heterocycles 38. Biocatalytic Synthesis of New Heterocyclic Mannich Bases and Derivatives

    Directory of Open Access Journals (Sweden)

    Denisa Leonte

    2015-07-01

    Full Text Available This paper describes the biocatalytic synthesis of new Mannich bases containing various heterocyclic rings (thiazole, furane, thiophene, pyridine by applying the lipase catalyzed trimolecular condensation of the corresponding heterocyclic aldehydes with acetone and primary aromatic amines, in mild and eco-friendly reaction conditions. The obtained Mannich bases were acylated to their corresponding N-acetyl derivatives. All compounds were characterized by 1H-NMR, 13C-NMR and MS spectrometry.

  1. [Scintigraphy of pulmonary ventilation with 99mTc-DTPA radio-aerosol. I. Semiotics of the static images].

    Science.gov (United States)

    Maini, C L; Bonetti, M G; Giordano, A; Pistelli, R; Antonelli Incalzi, R; Vecchioli, A; Galli, G

    1986-04-01

    Papers on deposition pattern analyses of radio-aerosol lung scans are few and not easy to interpret as there is a general lack of technical standardization and the number of patients studied is not large. Moreover these reports have been generally obtained with non-hydrosoluble radioaerosols. In the present study 43 patients underwent conventional pulmonary function testing and lung scanning using small droplet (equal or less than 2 microns) polydisperse 99mTc-DTPA radioaerosol produced with the "Settling Bag System"--Medi 400 (Sorin). The scans were analysed by two methods: a semiquantitative method proposed by Taplin (SQT); an original simpler semiquantitative method (SQM). Correlations of SQT and SQM with FEV1 and MEF75 resulted highly significant (p less than 0,001). SQM proved to be superior to SQT as far as reproducibility is concerned. The following conclusions can be drawn: small particle 99mTc-DTPA aerosol can be easily and cheaply produced by a commercial device; such a radioaerosol is well suited for the evaluation of small and large airways patency, as reflected by MEF75 and FEV1 respectively, with a diagnostic yield comparable to non-hydrosoluble radioaerosols; the original semiquantitative description of the deposition patterns proposed and validated in this study is quite easy to implement and it yields a high correlation with pulmonary function tests; moreover such an approach does not require digital data processing; the sensitivity of 99mTc-DTPA for the diagnosis of bronchial obstruction is very high and superior to routine pulmonary function tests; in addition the ventilation scan allows the topographical localization of the obstructions themselves.

  2. A new method based on Markov chains for deriving SB2 orbits directly from their spectra

    CERN Document Server

    Salomon, J -B; Guillout, P; Halbwachs, J -L; Arenou, F; Famaey, B; Lebreton, Y; Mazeh, T; Pourbaix, D; Tal-Or, L

    2012-01-01

    We present a new method to derive orbital elements of double-lined spectroscopic binaries (SB2). The aim is to have accurate orbital parameters of a selection of SB2 in order to prepare the exploitation of astrometric Gaia observations. Combined with our results, they should allow one to measure the mass of each star with a precision of better than 1%. The new method presented here consists of using the spectra at all epochs simultaneously to derive the orbital elements without templates. It is based on a Markov chain including a new method for disentangling the spectra.

  3. Synthesis, crystal structures and photoluminescence of anthracen- and pyrene-based coumarin derivatives.

    Science.gov (United States)

    Zhang, Hui; Tong, Hao; Zhao, Yuling; Yu, Tianzhi; Zhang, Peng; Li, Jianfeng; Fan, Duowang

    2015-11-01

    Two new anthracen- and pyrene-based coumarin derivatives, 3-(4-(anthracen-10-yl)phenyl)coumarin (4) and 3-(4-(pyrene-1-yl)phenyl)coumarin (5), were synthesized and characterized by FT-IR, (1)H NMR, element analysis and single crystal X-ray crystallography. The UV-vis absorption and photoluminescence spectra of these coumarin derivatives were investigated. The results show that compound 4 and 5 exhibit blue and blue-green emissions, respectively, under ultraviolet light excitation. Compared with the compound 4, the emission peak of compound 5 was bathochromically shifted by about 80 nm due to the more planar structure and larger π-conjugation.

  4. Capillary transfer constant of Gd-DTPA in the myocardium at rest and during vasodilation assessed by MRI

    DEFF Research Database (Denmark)

    Fritz-Hansen, T; Rostrup, Egill; Søndergaard, Lise

    1998-01-01

    The purpose of this study was to determine whether the capillary transfer constant (Ki) of gadolinium-DTPA was sensitive to perfusion changes and whether ischemic regions in the myocardium could be identified using the modified Kety formula. Ki was measured at rest and during dipyridamole......-induced vasodilation in 10 healthy volunteers and in 10 patients with ischemic heart disease. Ki increased by a factor of 2.5+/-1.2 (mean +/- SD) from 55+/-16 ml 100 g(-1)min(-1) at rest to 136+/-46 ml 100 g(-1)min(-1) (P

  5. Capillary permeability of 99mTc-DTPA in canine myocardium determined by intracoronary bolus injection and residue detection

    DEFF Research Database (Denmark)

    Svendsen, Jesper Hastrup; Efsen, F; Haunsø, S

    1989-01-01

    Capillary permeability of 99mtechnetium-diethylenetriaminepenta-acetic acid (99mTc-DTPA; MW 485.0) and 51chromium-ethylenediaminetetra-acetate (51Cr-EDTA; MW 340.2) was studied in an in vivo canine heart preparation by the single injection, residue detection (SIRD) method. In experiments on open...... chest dogs (group A) these indicators were administered separately as bolus injections into a cannulated diagonal branch of the left anterior descending coronary artery (LAD) and the curve of the response function was recorded by external activity registration. In further experiments on closed and open...

  6. Usefulness of sup(99m)Tc-DTPA renogram in the functional and diagnostic evaluations of chronic renal insufficiency in children

    Energy Technology Data Exchange (ETDEWEB)

    Matsueda, Y.; Hiraiwa, M.; Meguro, H.; Fujii, R. (Teikyo Univ., Tokyo (Japan). Faculty of Medicine)

    1980-09-01

    As there are some difficulties in the performance of renal biopsy and intravenous pyelography is of little use in children with chronic renal insufficiency, we evaluated the usefulness of sup(99m)Tc-DTPA renogram in two children. The causes of the renal insufficiency presented were obstructive congenital anomalies and chronic pyelonephritis in one patient and polycystic kidneys in the other. As expected, intravenous pyelography poorly visualized in the upper urinary tracts of both the cases examined. Sup(99m)Tc-DTPA renogram gave us distinct image for the diagnosis of the kidneys and urinary tracts, and the individual renal functions were clarified as well. It was apparently superior to the combined use of sup(99m)Tc-DMSA renoscintigram and /sup 131/I-Hippuran renogram in both the imaging and functional evaluations. In such cases as are reported herein, sup(99m)Tc-DTPA renogram might be a useful alternative to several other diagnostic tests.

  7. Detection of intrathoracic infectious lesions using {sup 111}In-diethylenetriamine pentaacetic bicyclic anhydride-IgG ({sup 111}In-DTPA-IgG) scintigraphy

    Energy Technology Data Exchange (ETDEWEB)

    Ota, Tomohiro; Goto, Hajime; Wada, Hiroo; Yuasa, Kazumi; Iguchi, Mari; Okamura, Tatsuru; Ieki, Ryuji; Suzuki, Kenzo [Tokyo Metropolitan Komagome General Hospital (Japan)

    1998-02-01

    The utility of {sup 111}In-DTPA-IgG imaging for the detection of intrathoracic lesions was evaluated in 10 patients with the suspicion of inflammatory or infectious diseases. They were intravenously administered 40 or 80 MBq of {sup 111}In-DTPA-IgG, and scanned after 24 or 48 hours. Of these, 8 cases, consisted of 4 cases with pneumonia and 2 cases with lung abscess and one case of pulmonary tuberculosis and one of a tuberculous pleuritis, showed true positive results. Others were one false negative case of pneumonia and one true negative case of lung cancer. Overall sensitivity and specificity were 88.9% and 100%, respectively. There were no cases which showed side effects or abnormal laboratory findings caused by the radiopharmaceuticals administered. Thus, {sup 111}In-DTPA-IgG imaging is a useful tool for the detection of intrathoracic infectious lesions. (author)

  8. A chaotic communication system of improved performance based on the Derivative-free nonlinear Kalman filter

    Science.gov (United States)

    Rigatos, Gerasimos

    2016-07-01

    The Derivative-free nonlinear Kalman Filter is used for developing a communication system that is based on a chaotic modulator such as the Duffing system. In the transmitter's side, the source of information undergoes modulation (encryption) in which a chaotic signal generated by the Duffing system is the carrier. The modulated signal is transmitted through a communication channel and at the receiver's side demodulation takes place, after exploiting the estimation provided about the state vector of the chaotic oscillator by the Derivative-free nonlinear Kalman Filter. Evaluation tests confirm that the proposed filtering method has improved performance over the Extended Kalman Filter and reduces significantly the rate of transmission errors. Moreover, it is shown that the proposed Derivative-free nonlinear Kalman Filter can work within a dual Kalman Filtering scheme, for performing simultaneously transmitter-receiver synchronisation and estimation of unknown coefficients of the communication channel.

  9. Non-invasive detection of biliary leaks using Gd-EOB-DTPA-enhanced MR cholangiography: comparison with T2-weighted MR cholangiography

    Energy Technology Data Exchange (ETDEWEB)

    Kantarci, Mecit; Pirimoglu, Berhan; Bayraktutan, Ummugulsum; Ogul, Hayri; Kizrak, Yesim; Eren, Suat [Atatuerk University, School of Medicine, Department of Radiology, Erzurum (Turkey); Karabulut, Nevzat [Pamukkale University, School of Medicine, Department of Radiology, Denizli (Turkey); Ozturk, Gurkan; Aydinli, Bulent [Atatuerk University, School of Medicine, Department of General Surgery, Erzurum (Turkey); Yilmaz, Sinan [Atatuerk University, School of Medicine, Department of Public Health, Erzurum (Turkey)

    2013-10-15

    To evaluate the added role of T1-weighted (T1w) gadolinium ethoxybenzyl diethylenetriamine penta-acetic acid (Gd-EOB-DTPA)-enhanced magnetic resonance cholangiography (MRC) compared with T2-weighted MRC (T2w-MRC) in the detection of biliary leaks. Ninety-nine patients with suspected biliary complications underwent routine T2w-MRC and T1w contrast-enhanced (CE) MRC using Gd-EOB-DTPA to identify biliary leaks. Two observers reviewed the image sets separately and together. MRC findings were compared with those of surgery and percutaneous transhepatic cholangiopancreatography. The sensitivity, specificity and accuracy of the techniques in identifying biliary leaks were calculated. Accuracy of locating biliary leaks was superior with the combination of Gd-EOB-DTPA-enhanced MRC and T2w-MRC (P < 0.05).The mean sensitivities were 79 % vs 59 %, and the mean accuracy rates were 84 % vs 58 % for combined CE-MRC and T2w-MRC vs sole T2w-MRC. Nineteen out of 21 patients with biliary-cyst communication, 90.4 %, and 12/15 patients with post-traumatic biliary extravasations, 80 %, were detected by the combination of Gd-EOB-DTPA-enhanced MRC and T2w-MRC images, P < 0.05. Gd-EOB-DTPA-enhanced MRC yields information that complements T2w-MRC findings and improves the identification and localisation of the bile extravasations (84 % accuracy, 100 % specificity, P < 0.05). We recommend Gd-EOB-DTPA-enhanced MRC in addition to T2w-MRC to increase the preoperative accuracy of identifying and locating extravasations of bile. (orig.)

  10. Structure-based designing of sordarin derivative as potential fungicide with pan-fungal activity.

    Science.gov (United States)

    Chakraborty, Biprashekhar; Sejpal, Nikunjkumar Vinodray; Payghan, Pavan V; Ghoshal, Nanda; Sengupta, Jayati

    2016-05-01

    Fungal infections have become a significant problem for immunosuppressed patients. Sordarin, a promising fungicidal agent, inhibits fungal protein synthesis by impairing elongation factor-2 (eEF2) function. Intriguingly, despite high sequence similarity among eEF2s from different species, sordarin has been shown to inhibit translation specifically in certain fungi while unable to do so in some other fungal species (e.g. Candida parapsilosis and Candida lusitaniae). The sordarin binding site on eEF2 as well as its mechanism of action is known. In a previous study, we have detailed the interactions between sordarin and eEF2 cavities from different fungal species at the molecular level and predicted the probable cause of sordarin sensitivity. Guided by our previous analysis, we aimed for computer-aided designing of sordarin derivatives as potential fungicidal agents that still remain ineffective against human eEF2. We have performed structural knowledge-based designing of several sordarin derivatives and evaluated predicted interactions of those derivatives with the sordarin-binding cavities of different eEF2s, against which sordarin shows no inhibitory action. Our analyses identify an amino-pyrrole derivative as a good template for further designing of promising broad-spectrum antifungal agents. The drug likeness and ADMET prediction on this derivative also supports its suitability as a drug candidate.

  11. Capillary permeability of 99mTc-DTPA and blood flow rate in the human myocardium determined by intracoronary bolus injection and residue detection

    DEFF Research Database (Denmark)

    Svendsen, Jesper Hastrup; Efsen, F; Haunsø, S

    1992-01-01

    of 99mTc-DTPA were quantitated during coronary angiography by the single injection, residue detection method. Eighteen patients undergoing a diagnostic coronary angiography because of uncharacteristic chest pain were studied. In 12 patients with angiographically normal epicardial coronary arteries...... myocardial blood flow rate in the human heart are in accordance with values obtained by other methods, and the PS product of 99mTc-DTPA is similar to results obtained in canine hearts. Localized coronary atheroma leading to insignificant coronary artery stenosis does not produce any measurable dysfunction...

  12. Quantitation of blood-brain barrier defect by magnetic resonance imaging and gadolinium-DTPA in patients with multiple sclerosis and brain tumors

    DEFF Research Database (Denmark)

    Larsson, H B; Stubgaard, M; Frederiksen, J L;

    1990-01-01

    In this study quantitation of the degree of deficiency of the blood-brain barrier (BBB) in patients with multiple sclerosis or brain tumors, by using MRI, is shown to be possible. As a measure of permeability of the BBB to Gadolinium-DTPA (Gd-DTPA) the flux per unit of distribution volume per unit......, and the results were comparable to results obtained from similar studies using positron emission tomography. The improved possibility of quantitating the defect of the BBB by MRI may give new information about pathogenesis or etiology, and leads to improved methods in monitoring the efficacy of treatments...

  13. MR angiography of collateral arteries in a hind limb ischemia model: comparison between blood pool agent Gadomer and small contrast agent Gd-DTPA.

    Directory of Open Access Journals (Sweden)

    Karolien Jaspers

    Full Text Available The objective of this study was to compare the blood pool agent Gadomer with a small contrast agent for the visualization of ultra-small, collateral arteries (diameter0.10. Inter-observer variation was 24% and 18% for Gadomer and Gd-DTPA, respectively. In conclusion, blood pool agent Gadomer improved vessel conspicuity compared to Gd-DTPA. Steady-state MRA can be considered as an excellent non-invasive alternative to intra-arterial XRA for the visualization of ultra-small collateral arteries.

  14. An elementary derivation of the hard/soft-acid/base principle.

    Science.gov (United States)

    Ayers, Paul W

    2005-04-08

    The hard/soft-acid/base (HSAB) principle indicates that hard acids prefer binding to hard bases (often forming bonds with substantial ionic character) while soft acids prefer binding to soft bases (often forming bonds with substantial covalent character). Though the HSAB principle is a foundational concept of the modern theory of acids and bases, the theoretical underpinnings of the HSAB principle remain murky. This paper examines the exchange reaction, wherein two molecules, one the product of reacting a hard acid and a soft base and the other the product of reacting a soft acid with a hard base, exchange substituents to form the preferred hard-hard and soft-soft product. A simple derivation shows that this reaction is exothermic, proving the validity of the HSAB principle. The analysis leads to the simple and conceptually appealing conclusion that the HSAB principle is a driven by simple electron transfer effects.

  15. Synthesis of indazole based diarylurea derivatives and their antiproliferative activity against tumor cell lines.

    Science.gov (United States)

    Zhao, Cui-rong; Wang, Rui-qi; Li, Gang; Xue, Xiao-xia; Sun, Chang-jun; Qu, Xian-jun; Li, Wen-bao

    2013-04-01

    New series of indazole based diarylureas were synthesized and their anticancer activity against cancer cells H460, A549, OS-RC-2, HT-29, Lovo, HepG2, Bel-7402, SGC-7901 and MDA-MB-231 were examined. These derivatives of diarylureas, except azaindazole based diarylureas 5f, 5l and 5m, showed superior or similar activity against most of these selected cancer cell lines to the reference compound sorafenib. The effect of substituents on the indazole ring was also investigated. Derivatives with trifluoromenthy or halogen substituent on the indazole ring showed higher activity against the selected cancer cell lines than sorafenib. The acute toxicity assay showed that compounds 5a, 5b and 5i possessed lower toxicity than sorafenib. Compound 5i with 4-(trifluoromenthy)-1H-indazole and 4-(trifluoromenthy) benzene moieties exhibited the most potent anticancer activity.

  16. Stereochemistry and spectroscopic analysis of bis-Betti base derivatives of 2,3-dihydroxynaphthalene.

    Science.gov (United States)

    Zamani, Mehdi; Shafiee, Mehdi; Keshavarz, Mohammad Hossein

    2016-04-01

    Density functional theory (DFT) was used to study the stereochemistry, thermodynamic stability, and spectra of recently synthesized bis-Betti base derivatives of 2,3-dihydroxynaphthalene obtained through multicomponent reactions of 2,3-dihydroxynaphthalene with aminoisoxazole and benzaldehyde derivatives. The stereochemistry of the products was investigated by theoretically calculating the infrared (IR) and proton nuclear magnetic resonance ((1)H NMR) spectra of the diastereomers and comparing them to the corresponding experimental data. The thermochemical properties of the reactions, including the enthalpy, internal energy, entropy, and Gibbs free energy, were also calculated. The diastereoselectivity of the reactions was estimated from the equilibrium distribution of diastereomers. According to the results, the synthesis of bis-Betti bases is exothermic and accompanied by a decrease in entropy. The energy difference between the diastereomers is quite small, but the Gibbs free energy change for the equilibrium syn anti favors the anti over syn configuration. These results are in good agreement with experimental observations.

  17. Excretion of purine base derivatives after intake of bacterial protein meal in pigs

    DEFF Research Database (Denmark)

    Hellwing, Anne Louise Frydendahl; Tauson, Anne-Helene; Skrede, A.

    2007-01-01

    Bacterial protein meal has a high content ofprotein but also of RNA and DNA. Sixteen barrows were allocated to four diets containing increasing levels of bacterial protein meal (BPM), from weaning to 80 kg live weight, to evaluate whether the RNA and DNA contents of BPM influenced the retention...... of nitrogen. It was hypothesised that an increased intake of RNA and DNA would lead to an increased urinary excretion of purine base derivatives and increased plasma concentrations. Retention of nitrogen was unaffected by dietary content of BPM (P=0.08) and the urinary excretion of purine base derivatives...... increased with increasing dietary content of BPM. No differences in fasting plasma concentration of uric acid, xanthine and hypoxanthine were observed. It can therefore be concluded that increasing levels of dietary BPM maintained protein accretion and led to changes in excretion of purine detrivatices...

  18. Interlaminar Stresses by Refined Beam Theories and the Sinc Method Based on Interpolation of Highest Derivative

    Science.gov (United States)

    Slemp, Wesley C. H.; Kapania, Rakesh K.; Tessler, Alexander

    2010-01-01

    Computation of interlaminar stresses from the higher-order shear and normal deformable beam theory and the refined zigzag theory was performed using the Sinc method based on Interpolation of Highest Derivative. The Sinc method based on Interpolation of Highest Derivative was proposed as an efficient method for determining through-the-thickness variations of interlaminar stresses from one- and two-dimensional analysis by integration of the equilibrium equations of three-dimensional elasticity. However, the use of traditional equivalent single layer theories often results in inaccuracies near the boundaries and when the lamina have extremely large differences in material properties. Interlaminar stresses in symmetric cross-ply laminated beams were obtained by solving the higher-order shear and normal deformable beam theory and the refined zigzag theory with the Sinc method based on Interpolation of Highest Derivative. Interlaminar stresses and bending stresses from the present approach were compared with a detailed finite element solution obtained by ABAQUS/Standard. The results illustrate the ease with which the Sinc method based on Interpolation of Highest Derivative can be used to obtain the through-the-thickness distributions of interlaminar stresses from the beam theories. Moreover, the results indicate that the refined zigzag theory is a substantial improvement over the Timoshenko beam theory due to the piecewise continuous displacement field which more accurately represents interlaminar discontinuities in the strain field. The higher-order shear and normal deformable beam theory more accurately captures the interlaminar stresses at the ends of the beam because it allows transverse normal strain. However, the continuous nature of the displacement field requires a large number of monomial terms before the interlaminar stresses are computed as accurately as the refined zigzag theory.

  19. χ measurements in Schiff’s base derivatives: Effect of metal nanoparticles

    Science.gov (United States)

    Sudheesh, P.; Chandrasekharan, K.

    2012-02-01

    The third-order nonlinear optical properties of Schiff's base derivatives and the effect of metal nanoparticles (Ag & Au) on their nonlinear optical response were studied. Colloidal metal nanoparticles were prepared by laser ablation. Z-scan technique with a 7ns Nd:YAG laser pulses at 532 nm were employed for the measurements. The compounds exhibit good optical limiting properties at the wavelength used. The materials used in the present work indicate that these materials are promising candidates for photonics applications.

  20. Synthesis and research of derived oxazol-5-ones based on α,β – unsaturated ketones

    Directory of Open Access Journals (Sweden)

    Сергей Александрович Петров

    2015-11-01

    Full Text Available The article deals with the production of new fluorescent dyes derived oxazol-5-ones based on α, β-unsaturated ketones, as well as confirmation of the structure of the compounds obtained using NMR and IR spectroscopy. The dyes of this series are relevant because one of the important practical problems in organic chemistry and chemical technology is currently seeking new fluorescent dyes for dyeing polyester materials and polymers

  1. Synthesis and research of derived oxazol-5-ones based on α,β – unsaturated ketones

    OpenAIRE

    Сергей Александрович Петров

    2015-01-01

    The article deals with the production of new fluorescent dyes derived oxazol-5-ones based on α, β-unsaturated ketones, as well as confirmation of the structure of the compounds obtained using NMR and IR spectroscopy. The dyes of this series are relevant because one of the important practical problems in organic chemistry and chemical technology is currently seeking new fluorescent dyes for dyeing polyester materials and polymers

  2. Tc-99m DTPA perfusion scintigraphy and color coded duplex sonography in the evaluation of minimal renal allograft perfusion

    Energy Technology Data Exchange (ETDEWEB)

    Bair, H.J.; Platsch, G.; Wolf, F. [Erlangen-Nuernberg Univ., Erlangen (Germany). Dept. of Nuclear Medicine; Guenter, E.; Becker, D. [Erlangen-Nuernberg Univ., Erlangen (Germany). Dept. of Internal Medicine 1; Rupprecht, H.; Neumayer, H.H. [Erlangen-Nuernberg Univ., Erlangen (Germany). Dept. of Internal Medicine 4

    1997-08-01

    Aim: The clinical impact of perfusion scintigraphy versus color coded Duplex sonography was evaluated, with respect to their potential in assessing minimal allograft perfusion in vitally threatened kidney transplants, i.e. oligoanuric allografts suspected to have either severe rejection or thrombosis of the renal vein or artery. Methods: From July 1990 to August 1994 the grafts of 15 out of a total of 315 patients were vitally threatened. Technetium-99m DTPA scintigraphy and color coded Duplex sonography were performed in all patients. For scintigraphic evaluation of transplant perfusion analog scans up to 60 min postinjection, and time-activity curves over the first 60 sec after injection of 370-440 MBq Tc-99m diethylenetriaminepentaacetate acid (DTPA) were used and classified by a perfusion score, the time between renal and iliac artery peaks (TDiff) and the washout of the renogram curve. Additionally, evaluation of excretion function and assessment of vascular or urinary leaks were performed. By color coded Duplex sonography the perfusion in all sections of the graft as well as the vascular anastomoses were examined and the maximal blood flow velocity (Vmax) and the resistive index (RI) in the renal artery were determined by means of the pulsed Doppler device. Pathologic-anatomical diagnosis was achieved by either biopsy or post-explant histology in all grafts. Results: Scintigraphy and color coded Duplex sonography could reliably differentiate minimal (8/15) and not perfused (7/15) renal allografts. The results were confirmed either by angiography in digital subtraction technique (DSA) or the clinical follow up. Conclusion: In summary, perfusion scintigraphy and color coded Duplex sonography are comparable modalities to assess kidney graft perfusion. In clinical practice scintigraphy and colorcoded Doppler sonography can replace digital subtraction angiography in the evaluation of minimal allograft perfusion. (orig.) [Deutsch] Ziel der Studie war es, das

  3. Synthesis and relaxivity of Gd(Ⅲ), Fe(Ⅲ) and Mn(Ⅱ)complexes with dihydropyridine derivative of diethylenetriaminepentaacetic acid

    Institute of Scientific and Technical Information of China (English)

    CHANG; Jianhua; ZHENG; Shuzhan; JIAN; Yajun; BAI; Yinjuan

    2005-01-01

    A novel ligand of DTPA-dihydropyridine derivative was synthesized by reaction of DTPA-dianhydride with 4-aniline-1,4-dihydropyridine. Its complexes of gadolinium, manganese and iron were prepared. Their spin-lattice relaxivities (T1) were investigated. The results show that the NMR T1 relaxivitives (R1) for complexes of Fe(Ⅲ), Mn(Ⅱ) are less than that of Gd(Ⅲ) complex,which has a high relaxivity (R1) on the surrounding water protons, indicating that the Gd(Ⅲ) complex possesses the precondition to be contrast agents for magnetic resonance imaging.

  4. Fluorescence Properties and Electrochemical Behavior of Some Schiff Bases Derived from N-Aminopyrimidine.

    Science.gov (United States)

    Gulcan, Mehmet; Doğru, Ümit; Öztürk, Gülsiye; Levent, Abdulkadir; Akbaş, Esvet

    2014-03-01

    A series of Schiff bases (L 1 , L 2 and L 3) were prepared by refluxing aromatic aldehydes with N-Aminopyrimidine derivatives in methanol and ethanol. The structures of synthesized compounds were characterized by FTIR, (1)H NMR, (13)C NMR and microanalysis. The electrochemical behaviors of the Schiff base ligands were also discussed. Moreover, the evaluation of absorption and emission properties of the structures were carried out in five different solvents. The products show visible absorption maxima in the range of 304-576 nm, and emission maxima from 636 to 736 nm in all solvents tested.

  5. The scenario-based approach adopted in the ELECTRA project for deriving innovate control room functionality

    DEFF Research Database (Denmark)

    Marinelli, Mattia; Heussen, Kai; Prostejovsky, Alexander

    2017-01-01

    . The paper intends to outline the methodology adopted, which is based on the cognitive work analysis framework, to provide an overview of the most interesting scenarios and to summarize the requirements analysis results. In order to derive required control room functionality, a set of relevant control room...... scenarios have been identified based on the Web-of-Cells control concept. We considered scenarios that challenge traditional control schemes, scenarios that caused major failures (i.e., blackouts) and scenarios that can be expected to appear in the future. For each scenario, information concerning network...

  6. Synthesis of vegetable oil based polyol with cottonseed oil and sorbitol derived from natural source

    Institute of Scientific and Technical Information of China (English)

    Lian Kun Jia; Li Xiang Gong; Wen Jiao Ji; Cheng You Kan

    2011-01-01

    In order to prepare the polyol with all bio-based components as raw materials, cottonseed oil was first epoxidized by peroxyformic acid generated in situ from hydrogen peroxide and formic acid, and the cottonseed oil based polyols with variable hydroxyl value were then prepared by the ring-opening of epoxidized cottonseed oil with sorbitol, which is a multi-functional hydroxyl compound derived from a natural source. The chemical structure of the products was characterized with FTIR analysis, and the residual epoxy oxygen content and hydroxyl value of the polyol versus the ring-opening time were investigated.

  7. Synthesis, crystal structure and redox properties of dihydropyrazole-bridged ferrocene-based derivatives

    Science.gov (United States)

    Li, Heng-Dong; Ma, Zai-He; Yang, Kun; Xie, Li-Li; Yuan, Yao-Feng

    2012-09-01

    Dihydropyrazole-bridged ferrocene-based derivatives were prepared by corresponding chalcones with hydrazine hydrate, then acylation with 3-(ethoxycarbonyl)propionyl chloride directly in high yields and purity. All of these compounds were characterized by MS, IR, 1H NMR, 13C NMR and elemental analysis. The relationship between the structure and redox properties was investigated based on the results of single crystal X-ray structure determinations and cyclic voltammetry. The mechanism of the electron transfer for representative compound 4b was verified by density functional theory (DFT) calculations.

  8. Design of quadrature mirror filter bank using Lagrange multiplier method based on fractional derivative constraints

    Directory of Open Access Journals (Sweden)

    B. Kuldeep

    2015-06-01

    Full Text Available Fractional calculus has recently been identified as a very important mathematical tool in the field of signal processing. Digital filters designed by fractional derivatives give more accurate frequency response in the prescribed frequency region. Digital filters are most important part of multi-rate filter bank systems. In this paper, an improved method based on fractional derivative constraints is presented for the design of two-channel quadrature mirror filter (QMF bank. The design problem is formulated as minimization of L2 error of filter bank transfer function in passband, stopband interval and at quadrature frequency, and then Lagrange multiplier method with fractional derivative constraints is applied to solve it. The proposed method is then successfully applied for the design of two-channel QMF bank with higher order filter taps. Performance of the QMF bank design is then examined through study of various parameters such as passband error, stopband error, transition band error, peak reconstruction error (PRE, stopband attenuation (As. It is found that, the good design can be obtained with the change of number and value of fractional derivative constraint coefficients.

  9. Derivation of Relationships between Spectral Vegetation Indices from Multiple Sensors Based on Vegetation Isolines

    Directory of Open Access Journals (Sweden)

    Kenta Obata

    2012-02-01

    Full Text Available An analytical form of relationship between spectral vegetation indices (VI is derived in the context of cross calibration and translation of vegetation index products from different sensors. The derivation has been carried out based on vegetation isoline equations that relate two reflectance values observed at different wavelength ranges often represented by spectral band passes. The derivation was first introduced and explained conceptually by assuming a general functional form for VI model equation. This process is universal by which two VIs of different sensors and/or different model equations can be related conceptually. The general process was then applied to the actual case of normalized difference vegetation index (NDVI from two sensors in a framework of inter-sensor continuity. The derivation results indicate that the NDVI from one sensor can be approximated by a rational function of NDVI from the other sensor as a parameter. Similar result was obtained for the case of soil adjusted VI, enhanced VI, and two-band variance of enhanced VI.

  10. Facial Expression Recognition Based on Features Derived From the Distinct LBP and GLCM

    Directory of Open Access Journals (Sweden)

    Gorti Satyanarayana Murty

    2014-01-01

    Full Text Available Automatic recognition of facial expressions can be an important component of natural human-machine interfaces; it may also be used in behavioural science and in clinical practice. Although humans recognise facial expressions virtually without effort or delay, reliable expression recognition by machine is still a challenge. This paper, presents recognition of facial expression by integrating the features derived from Grey Level Co-occurrence Matrix (GLCM with a new structural approach derived from distinct LBP’s (DLBP ona 3 x 3 First order Compressed Image (FCI. The proposed method precisely recognizes the 7 categories of expressions i.e.: neutral, happiness, sadness, surprise, anger, disgust and fear. The proposed method contains three phases. In the first phase each 5 x 5 sub image is compressed into a 3 x 3 sub image. The second phase derives two distinct LBP’s (DLBP using the Triangular patterns between the upper and lower parts of the 3 x 3 sub image. In the third phase GLCM is constructed based on the DLBP’s and feature parameters are evaluated for precise facial expression recognition. The derived DLBP is effective because it integrated with GLCM and provides better classification performance. The proposed method overcomes the disadvantages of statistical and formal LBP methods in estimating the facial expressions. The experimental results demonstrate the effectiveness of the proposed method on facial expression recognition.

  11. Synthesis of Some Novel Heterocyclic and Schiff Base Derivatives as Antimicrobial Agents

    Directory of Open Access Journals (Sweden)

    Mohamed E. Azab

    2015-10-01

    Full Text Available Treatment of 2,3-diaryloxirane-2,3-dicarbonitriles 1a–c with different nitrogen nucleophiles, e.g., hydrazine, methyl hydrazine, phenyl hydrazine, hydroxylamine, thiosemicarbazide, and/or 2-amino-5-phenyl-1,3,4-thiadiazole, afforded pyrazole, isoxazole, pyrrolotriazine, imidazolothiadiazole derivatives 2–5, respectively. Reacting pyrazoles 2a–c with aromatic aldehydes and/or methyl glycinate produced Schiff’s bases 7a–d and pyrazolo[3,4-b]-pyrazinone derivative 8, respectively. Treating 7 with ammonium acetate and/or hydrazine hydrate, furnished the imidazolopyrazole and pyrazolotriazine derivatives 9 and 10, respectively. Reaction of 8 with chloroacetic acid and/or diethyl malonate gave tricyclic compound 11 and triketone 12, respectively. On the other hand, compound 1 was reacted with active methylene precursors, e.g., acetylacetone and/or cyclopentanone producing adducts 14a,b which upon fusion with ammonium acetate furnished the 3-pyridone derivatives 15a,b, respectively. Some of newly synthesized compounds were screened for activity against bacterial and fungal strains and most of the newly synthesized compounds showed high antimicrobial activities. The structures of the new compounds were elucidated using IR, 1H-NMR, 13C-NMR and mass spectroscopy.

  12. Lightweight and Distributed Connectivity-Based Clustering Derived from Schelling's Model

    Science.gov (United States)

    Tsugawa, Sho; Ohsaki, Hiroyuki; Imase, Makoto

    In the literature, two connectivity-based distributed clustering schemes exist: CDC (Connectivity-based Distributed node Clustering scheme) and SDC (SCM-based Distributed Clustering). While CDC and SDC have mechanisms for maintaining clusters against nodes joining and leaving, neither method assumes that frequent changes occur in the network topology. In this paper, we propose a lightweight distributed clustering method that we term SBDC (Schelling-Based Distributed Clustering) since this scheme is derived from Schelling's model — a popular segregation model in sociology. We evaluate the effectiveness of the proposed SBDC in an environment where frequent changes arise in the network topology. Our simulation results show that SBDC outperforms CDC and SDC under frequent changes in network topology caused by high node mobility.

  13. Development of {sup 177}Lu-DTPA-SPIO conjugates for potential use as a dual contrast SPECT/MRI imaging agent

    Energy Technology Data Exchange (ETDEWEB)

    Shanehsazzadeh, Saeed; Yousefnia, Hassan [Nuclear Science and Technology Research Institute (NSTRI), Tehran (Iran, Islamic Republic of); Gruettner, Cordula [Micromod Partikeltechnologie GmbH, Rostock (Germany); and others

    2016-08-01

    This study describes the preparation, biodistribution of {sup 177}Lu-DTPA-SPIO after intravenous injection in rats. The chelator DTPA dianhydride was conjugated to SPIO NPs using a small modification of the well-known cyclic anhydride method. Conjugation was done at a 1:2 (SPIO:ccDTPA) molar ratio. Conjugation reaction was purified with Magnetic assorting column (MACs) using high gradient magnetic field following incubation, the radio labeled conjugate was checked using RTLC method for labeling and purity checked. The RTLC showed that labeling yield was above 99% after purification and the compound have good in-vitro stabilities until 48 h post injection in the presence of human serum. The biodistribution of {sup 177}Lu-DTPA-SPIO in rats showed dramatic uptake in the reticuloendothelial system (RES) and their clearance is so fast in other organs especially in the blood. In conclusion, due to high uptakes of this radiotracer in the liver and spleen and their fast clearance from other tissues, especially in blood, it is suggested that this radiotracer would be suitable for RES studies.

  14. The correlation between effective renal plasma flow (ERPF) and glomerular filtration rate (GFR) with renal scintigraphy 99mTc-DTPA study

    Science.gov (United States)

    Ratnasari, D.; Nazir, F.; Toresano, L. O. H. Z.; Pawiro, S. A.; Soejoko, D. S.

    2016-03-01

    The prevalence of chronic renal diseases in Indonesia has an increasing annual trend, because it is frequently unrecognized and often co-exists with other disease. GFR and ERPF are parameters currently utilized to estimate renal function at routine renal scintigraphy 99m-Tc DTPA study. This study used 99m-Tc DTPA to measure GFR and ERPF. The purpose of this study was to find the correlation between ERPF and GFR, for ERPF analysis with Schlegel's method, and GFR analysis with Gate's method, as well as to find correction factor between both variables. Analysis of renal scintigraphy has been performed at Department of Nuclear Medicine Pertamina Center Hospital to thirty patient images acquired from 2014 to 2015 which were analyzed retrospectively data, using gamma camera dual head with counting method from renal scintigraphy 99m-Tc DTPA study. The calculation was executed by means of both display and manual calculation. Pearson's statistical analysis resulted on Positive Correlation for all data, with ERPF and GFR (display) showing Strongly Positive Correlation (r = 0.82; p- value < 0.05). Standard deviation was found to be 27.58 and 107.64 for GFR and ERPF (display), respectively. Our result indicated that the use of 99mTc-DTPA measure ERPF was not recommended.

  15. Uptake of indium-111 in the liver of mice following administration of indium-111-DTPA-labeled monoclonal antibodies: Influence of labeling parameters, physiologic parameters, and antibody dose

    Energy Technology Data Exchange (ETDEWEB)

    Schuhmacher, J.; Klivenyi, G.; Matys, R.; Kirchgebner, H.; Hauser, H.; Maier-Borst, W.; Matzku, S. (Institute of Radiology and Pathophysiology, Heidelberg (Germany, F.R.))

    1990-06-01

    Liver uptake of indium-111 ({sup 111}In) in mice was investigated following administration of {sup 111}In-DTPA murine monoclonal antibodies ({sup 111}In-DTPA-MAbs) labeled by the cyclic anhydride method. Biodistribution of HPLC-purified {sup 111}In-DTPA-MAb preparations was checked with a low (0.2 micrograms) and a high (8.0 micrograms) MAb dose. Using Bio Gel P-30 for desalting the MAb-conjugates, {sup 111}In uptake in the liver amounted to 8%-9% of the injected dose (ID) and was independent from the MAb dose, the DTPA-to-MAb molar ratio, tumor growth and biologic variability (different MAbs and different strains of mice). Using Sephadex G-25 for desalting, 0.2 micrograms doses from 7 out of 26 preparations showed increased liver accumulation of {sup 111}In in non-tumor mice ranging from 15%-25% of ID. Corresponding high doses led to a normal value of 8%-9%. Increased liver uptake of the low dose could not be reduced by coadministration of the unconjugated MAb, but was normal after reinjection of in vivo filtered material. An inverse intracellular distribution of {sup 111}In activity between sediment and supernatant of liver homogenates, following the administration of the low and the high MAb dose, indicated an artifact of the labeling procedure rather than an inherent biological property of labeled MAbs.

  16. Large regenerative nodules in a patient with Budd-Chiari syndrome after TIPS positioning while on the liver transplantation list diagnosed by Gd-EOB-DTPA MRI

    Institute of Scientific and Technical Information of China (English)

    Matteo Renzulli; Vincenzo Lucidi; Cristina Mosconi; Chiara Quarneti; Emanuela Giampalma; Rita Golfieri

    2011-01-01

    BACKGROUND: Large regenerative nodules (LRNs) are hyperplastic benign nodules most commonly associated with Budd-Chiari syndrome (BCS), caused by outflow obstruction of the hepatic veins or vena cava. To our knowledge, no cases of LRNs arising in BCS after transjugular intrahepatic portosystemic shunt (TIPS) positioning and detected by Gd-EOB-DTPA MRI have been reported in the literature. METHODS: A 58-year-old woman with BCS, on the liver transplantation (LT) list, underwent a follow-up enhanced MRI. Two years earlier, a TIPS had been placed. In 2008, recurrent hepaticoencephalopathy resistant to medical treatment fulfilled the LT criteria for BCS treated with TIPS and the patient was therefore added to the LT list. CT performed before TIPS had not detected any hepatic lesions. CT performed six months after TIPS showed its complete patency but documented two indeterminate hypervascular liver lesions. RESULTS: MRI performed with Gd-EOB-DTPA revealed additional hypervascular lesions with uptake and retention of the medium in the hepatobiliary phase, thus reflecting a benign behavior of hepatocellular composition. These MRI features were related to LRNs as confirmed by histopathologic analysis. CONCLUSIONS: Gd-EOB-DTPA-enhanced MRI is potentially superior to standard imaging using gadolinium chelates or spiral CT, especially for the differential diagnosis of hypervascular lesions. Gd-EOB-DTPA MRI may become the imaging method of choice for evaluating LT list patients with BCS after TIPS placement.

  17. Preparation and preliminary biological evaluation of radiogallium-labeled DTPA-amlodipine complex for possible L-type calcium channel imaging

    Energy Technology Data Exchange (ETDEWEB)

    Firuzyar, Tahereh; Shafiee-Ardestani, Mehdi; Khalaj, Ali [Tehran University of Medical Sciences, Tehran (Iran, Islamic Republic of). Faculty of Pharmacy; Jalilian, Amir R.; Fazaeli, Yousef; Aboudzadeh, Mohammad Reza [Nuclear Science and Technology Research Institute (NSTRI), Tehran (Iran, Islamic Republic of). Radiopharmacy Research Group

    2014-07-01

    A DTPA-conjugated amlodipine analog (DTPA-AMLO) 3, was prepared for possible voltage gated calcium channel imaging after radiolabeling with Ga-67. [{sup 67}Ga]-DTPA-AMLO complex was prepared starting [{sup 67}Ga]gallium chloride and DTPA-AMLO in 60-90 min at 50-60 C in phosphate buffer. The partition co-efficient and stability of the tracer was determined in final solution (25 C) and presence of human serum (37 C) up to 24 h. The biodistribution of the labeled compound in wild-type rats were determined up to 72 h using organ counting and SPECT. The radiolabled complex was prepared in high radiochemical purity (>96%, RTLC and >98% HPLC) and significant specific activity (7-10 GBq/mmol). The log P for the complex was calculated as -0.594, consistent with a water soluble complex. The tracer is mostly washed out through kidneys which were in full compliance with the amlodipine metabolism and imaging studies demonstrated the same behavior. The tracer uptake in organs with smooth muscles was observed in stomach, colon as well as intestine.

  18. Synthesis, Characterization and Antimicrobial Activities of Some New Heterocyclic Schiff Bases Derived from Thiocarbohydrazide.

    Science.gov (United States)

    El-Mahdy, Kamelia; El-Kazak, Azza; Abdel-Megid, Mohamed; Seada, Magdyand; Farouk, Osama

    2016-01-01

    The reaction of prazolobenzothienopyrimidine-3-carbaldehyde 1 with thiocarbohydrazide afforded the Schiff's base 3. The latter compound reacted with some electrophilic reagents to give 1,2,4-triazoles 4-6 and 1,2,4-triazines 7-9. Treatment of compound 3 with 2-cyano-3,3-bis(methylthio)acrylonitrile gave the corresponding 5-amino-4-cyano-3-methylthiopyrazole derivative 11. The reaction of pyrazole 11 with carbon disulfide afforded dithioxopyrazolopyrimidine 12. Acylation of compound 11 by using acetic anhydride yielded acetamide 13. On the other hand, the cyclocondensation of pyrazole 11 with acetic anhydride in pyridine yielded pyrazolopyrimidine derivative 14. The reactivity of compound 11 towards formamide and phenylisothiocyanate to give the pyrazolopyrimidines 15 and 16 was studied. The newly synthesized compounds were screened for their antimicrobial activity.

  19. Fingerprint Image Enhancement Based on Second Directional Derivative of the Digital Image

    Directory of Open Access Journals (Sweden)

    Onnia Vesa

    2002-01-01

    Full Text Available This paper presents a novel approach of fingerprint image enhancement that relies on detecting the fingerprint ridges as image regions where the second directional derivative of the digital image is positive. A facet model is used in order to approximate the derivatives at each image pixel based on the intensity values of pixels located in a certain neighborhood. We note that the size of this neighborhood has a critical role in achieving accurate enhancement results. Using neighborhoods of various sizes, the proposed algorithm determines several candidate binary representations of the input fingerprint pattern. Subsequently, an output binary ridge-map image is created by selecting image zones, from the available binary image candidates, according to a MAP selection rule. Two public domain collections of fingerprint images are used in order to objectively assess the performance of the proposed fingerprint image enhancement approach.

  20. Origami mechanical metamaterials based on the Miura-derivative fold patterns

    Science.gov (United States)

    Zhou, Xiang; Zang, Shixi; You, Zhong

    2016-07-01

    This paper presents two new types of origami-inspired mechanical metamaterials based on the Miura-derivative fold patterns that consist of non-identical parallelogram facets. The analytical models to predict dimension changes and deformation kinematics of the proposed metamaterials are developed. Furthermore, by modelling the creases as revolute hinges with certain rotational spring constants, we derived analytical models for stretching and bulk moduli. The analytical models are validated through finite-element simulation results. Numerical examples reveal that the proposed metamaterials possess some intriguing properties, including negative Poisson's ratios and bulk modulus. The work presented in this paper can provide a highly flexible framework for the design of versatile tunable mechanical metamaterials.

  1. Deformation analysis of polymers composites: rheological model involving time-based fractional derivative

    DEFF Research Database (Denmark)

    Zhou, H. W.; Yi, H. Y.; Mishnaevsky, Leon

    2016-01-01

    A modeling approach to time-dependent property of Glass Fiber Reinforced Polymers (GFRP) composites is of special interest for quantitative description of long-term behavior. An electronic creep machine is employed to investigate the time-dependent deformation of four specimens of dog......-bond-shaped GFRP composites at various stress level. A negative exponent function based on structural changes is introduced to describe the damage evolution of material properties in the process of creep test. Accordingly, a new creep constitutive equation, referred to fractional derivative Maxwell model...... by the fractional derivative Maxwell model proposed in the paper are in a good agreement with the experimental data. It is shown that the new creep constitutive model proposed in the paper needs few parameters to represent various time-dependent behaviors....

  2. Induced cholesteric systems based on some cyano derivatives as host phases

    Science.gov (United States)

    Shkolnikova, Natalya I.; Kutulya, Lidiya A.; Vashchenko, V. V.; Fedoryako, A. P.; Lapanik, V. I.; Posledovich, N. R.

    2002-12-01

    Macroscopical properties of some induced cholesteric compositions based on 4-pentyl-4'-cyano derivatives of biphenyl and phenylcyclohexane as host phases have been investigated. The series of N-arylidene derivatives of (S)-1-phenylethylamine with varied both rigid moiety of the N-arylidene fragment and terminal substituent was used as chiral dopants. The influence of the chiral dopant molecular structure as well as of physical properties of the host phases used on the helical twisting power, the temperature dependence of the induced helical pitch and the N* mesophase thermal stability has been characterized. It has been concluded that the distinctions in properties of the LC systems containing the OCH2 and COO linking groups are caused by their different conformational states.

  3. Luminescent liquid crystalline materials based on palladium(II) imine derivatives containing the 2-phenylpyridine core.

    Science.gov (United States)

    Micutz, Marin; Iliş, Monica; Staicu, Teodora; Dumitraşcu, Florea; Pasuk, Iuliana; Molard, Yann; Roisnel, Thierry; Cîrcu, Viorel

    2014-01-21

    In this work we report our studies concerning the synthesis and characterisation of a series of imine derivatives that incorporate the 2-phenylpyridine (2-ppy) core. These derivatives were used in the cyclometalating reactions of platinum(II) or palladium(II) in order to prepare several complexes with liquid crystalline properties. Depending on the starting materials used as well as the solvents employed, different metal complexes were obtained, some of them showing both liquid crystalline behaviour and luminescence properties at room temperature. It was found that, even if there are two competing coordination sites, the cyclometalation process takes place always at the 2-ppy core with (for Pt) or without (for Pd) the imine bond cleavage. We successfully showed that it is possible to prepare emissive room temperature liquid crystalline materials based on double cyclopalladated heteroleptic complexes by varying the volume fraction of the long flexible alkyl tails on the ancillary benzoylthiourea (BTU) ligands.

  4. Associations between the uptake of {sup 111}In-DTPA-trastuzumab, HER2 density and response to trastuzumab (Herceptin) in athymic mice bearing subcutaneous human tumour xenografts

    Energy Technology Data Exchange (ETDEWEB)

    McLarty, Kristin; Cornelissen, Bart; Scollard, Deborah A. [University of Toronto, Department of Pharmaceutical Sciences, Toronto, ON (Canada); Done, Susan J. [University of Toronto, Department of Medical Biophysics, Toronto, ON (Canada)]|[University of Toronto, Department of Laboratory Medicine and Pathobiology, Toronto, ON (Canada)]|[University Health Network, Department of Pathology, Toronto, ON (Canada); Chun, Kathy [North York General Hospital, Genetics Program, Toronto, ON (Canada); Reilly, Raymond M. [University of Toronto, Department of Pharmaceutical Sciences, Toronto, ON (Canada)]|[University of Toronto, Department of Medical Imaging, Toronto, ON (Canada)]|[University Health Network, Toronto General Research Institute, Toronto, ON (Canada)]|[University of Toronto, Leslie Dan Faculty of Pharmacy, Toronto, ON (Canada)

    2009-01-15

    The purpose of the study was to investigate the associations between uptake of {sup 111}In-DTPA-trastuzumab, tumour HER2 density and response to trastuzumab (Herceptin) of human breast cancer (BC) xenografts in athymic mice. The tumour uptake of {sup 111}In-DTPA-trastuzumab in athymic mice bearing BC xenografts with increasing HER2 density (0 to 3+) was evaluated. Specific uptake ratios were established in biodistribution (SUR) and imaging studies (ROI-SUR) using {sup 111}In-labeled mouse IgG ({sup 111}In-DTPA-mIgG). Further corrections were made for circulating radioactivity using tumour-to-blood ratios defined as a localization index (LI) and region-of-interest localization index (ROI-LI), respectively. Mice were treated with trastuzumab (Herceptin). A tumour growth inhibition index (TGI) was calculated and relative TGIs calculated by dividing the TGI of control by that of trastuzumab-treated mice. Strong, nonlinear associations with HER2 density were obtained if the uptake of {sup 111}In-DTPA-trastuzumab was corrected for nonspecific IgG localization (i.e., SUR; r{sup 2}=0.99) and circulating radioactivity (i.e., LI; r{sup 2} =0.87), but without these corrections, the association between HER2 density and tumour uptake was poor (r{sup 2}=0.22). There was a strong association between ROI-SUR and ROI-LI values and HER2 expression (r{sup 2}=0.90 and r{sup 2}=0.95), respectively. All tumours were imaged. Relative TGI values were associated with increasing uncorrected tumour uptake of {sup 111}In-DTPA-trastuzumab but not always with HER2 density (i.e., MCF-HER2-18 cells with trastuzumab-resistance). HER2 expression (0 to 3+) can be differentiated using {sup 111}In-DTPA-trastuzumab, but requires correction of tumour uptake for nonspecific IgG localization and circulating radioactivity. The uncorrected uptake of {sup 111}In-DTPA-trastuzumab was associated with tumour response to trastuzumab. (orig.)

  5. Variability in soil micronutrients extracted by DTPA and Mehlich-3 at the plot scale in an acidic environment

    Science.gov (United States)

    Paz-Ferreiro, Jorge; Lado, Marcos; de Abreu, Cleide A.

    2014-05-01

    Land use practices affect soil properties and nutrient supply. Very limited data are available on micronutrient extractability in northwest Spain. The aim of this study was to analyse long-term effects of land use on the concentration, variability and spatial distribution of soil nutrients. To this end, neighboring forest and cultivated stands were compared. The study was carried out in an acid, rich in organic matter soil developed over sediments at the province of Lugo, northwestern of Spain. Adjacent plots with a surface of 100 m2 were marked on regular square grids with 2-m spacing. Fe, Mn, Zn and Cu were extracted both by Mehlich-3 and DTPA solutions and determined by ICP-MS. General soil chemical and physical properties were routinely analyzed. In arable land, microelement concentration ranges were as follows: Fe (100 and 135 mg kg-1), Mn (7.6 and 21.5 mg kg-1), Zn (0.6 and 3.7 mg kg-1), and Cu (0.2 and 0.7 mg kg-1). In forest land, these ranges were: Fe (62 and 309 mg kg-1), Mn (0.2 and 2.1 mg kg-1), Zn (0.2 and 2.9 mg kg-1), and Cu (0.1 and 0.2 mg kg-1). With the exception of Fe-DTPA, microelement concentrations extracted both with DTPA and Mehlich-3 were higher in the cultivated than in the forest stand. Coefficients of variation were higher for the microelement content of the soil under forest. Principal component analysis was performed to evaluate associations between extractable microelements and general physico-chemical properties. At the studied scale, nutrient management was the main factor affecting the agricultural site, whereas soil-plant interactions were probably responsible for the higher variation within the forest site. Patterns of spatial variability of the studied nutrients at the small plot scale also were assessed by geostatistical techniques. Results were discussed in the frame of sustainable land use and organic matter decline with conventional tillage and sustainable land use

  6. Phytoceramide and sphingoid bases derived from brewer's yeast Saccharomyces pastorianus activate peroxisome proliferator-activated receptors

    Directory of Open Access Journals (Sweden)

    Mitsutake Susumu

    2011-08-01

    Full Text Available Abstract Background Peroxisome proliferator-activated receptors (PPARs are ligand-activated transcription factors that regulate lipid and glucose metabolism. PPARα is highly expressed in the liver and controls genes involved in lipid catabolism. We previously reported that synthetic sphingolipid analogs, part of which contains shorter-length fatty acid chains than natural sphingolipids, stimulated the transcriptional activities of PPARs. Sphingosine and dihydrosphingosine (DHS are abundant sphingoid bases, and ceramide and dihydroceramide are major ceramide species in mammals. In contrast, phytosphingosine (PHS and DHS are the main sphingoid bases in fungi. PHS and phytoceramide exist in particular tissues such as the epidermis in mammals, and involvement of ceramide species in PPARβ activation in cultured keratinocytes has been reported. The purpose of the present study is to investigate whether natural sphingolipids with C18 fatty acid and yeast-derived sphingoid bases activate PPARs as PPAR agonists. Method Lipids of brewer's yeast contain PHS- and DHS-based sphingolipids. To obtain the sphingoid bases, lipids were extracted from brewer's yeast and acid-hydrolyzed. The sphingoid base fraction was purified and quantified. To assess the effects of sphingolipids on PPAR activation, luciferase reporter assay was carried out. NIH/3T3 and human hepatoma (HepG2 cells were transfected with expression vectors for PPARs and retinoid × receptors, and PPAR responsive element reporter vector. When indicated, the PPAR/Gal4 chimera system was performed to enhance the credibility of experiments. Sphingolipids were added to the cells and the dual luciferase reporter assay was performed to determine the transcriptional activity of PPARs. Results We observed that phytoceramide increased the transcriptional activities of PPARs significantly, whereas ceramide and dihydroceramide did not change PPAR activities. Phytoceramide also increased transactivation of

  7. Differential renal function measured by 99Tcm-DTPA and 99Tcm-DMSA in a complete unilateral renal obstruction rat model.

    Science.gov (United States)

    van de Wiele, C; Everaert, K; van der Eecken, H; van Haelst, J P; Simons, M; Dierckx, R A

    1997-11-01

    We performed a prospective study to determine whether 99Tcm-DTPA differential renal function (DRF) in the case of acute obstruction in a unipapillary kidney rat model shows a similar pattern of results as 99Tcm-DMSA in a multipapillary kidney model, and to exclude recirculation or a block of tubular transport of DMSA which might explain the discrepancy reported by Kelleher et al. In 15 male and 4 female Wistar rats (weight 300-350 g), 99Tcm-DMSA renal uptake was measured 24 h before complete obstruction of the right ureter, which served as reference values. Twenty-four hours after obstruction, 99Tcm-DTPA (18.5 MBq) renography and 99Tcm-DMSA (111 MBq) non-depth-corrected renal uptake measurements were performed in the 15 male rats; in the 4 female rats, 740 MBq of 99Tcm-DMSA were injected and absolute, non-depth-corrected renal uptake measured at 24, 48 and 72 h. 99Tcm-DMSA DRF of the right kidney ranged from 48 to 55% (mu: 51%; sigma: 2%) before obstruction and from 14 to 35% (mu: 24%; sigma: 6%) after obstruction, whereas 99Tcm-DTPA DRF ranged from 16 to 30% (mu: 25%; sigma: 4%). No significant differences were found between DRF measured by 99Tcm-DMSA and 99Tcm-DTPA (P = 0.18), or between DRF of the obstructed kidney as measured by 99Tcm-DMSA at 24, 48 and 72 h (P > 0.2). Hypothetically, the discrepancy between our findings and those of Kelleher et al. may be due to intratubular pressure. In conclusion, the present findings and those of Kelleher et al. suggest the differences in DRF following complete unilateral renal obstruction, as determined by DTPA and DMSA, are probably species-specific. Furthermore, recirculation and block of tubular transport are unlikely to contribute significantly to differences in DRF as measured by the two radiopharmaceuticals.

  8. Gd-DTPA T1 relaxivity in brain tissue obtained by convection-enhanced delivery, magnetic resonance imaging and emission spectroscopy

    Energy Technology Data Exchange (ETDEWEB)

    Haar, Peter J [Department of Anatomy and Neurobiology, Virginia Commonwealth University, Richmond, VA (United States); Broaddus, William C; Chen Zhijian; Gillies, George T [Department of Neurosurgery, Virginia Commonwealth University, Richmond, VA (United States); Fatouros, Panos P; Corwin, Frank D, E-mail: wbroaddus@mcvh-vcu.ed [Department of Radiology, Virginia Commonwealth University, Richmond, VA (United States)

    2010-06-21

    A common approach to quantify gadolinium (Gd) contrast agents involves measuring the post-contrast change in T1 rate and then using the constant T1 relaxivity R to determine the contrast agent concentration. Because this method is fast and non-invasive, it could be potentially valuable in many areas of brain research. However, to accurately measure contrast agent concentrations in the brain, the T1 relaxivity R of the specific agent must be accurately known. Furthermore, the macromolecular content and compartmentalization of the brain extracellular space (ECS) are expected to significantly alter R from values measured in aqueous solutions. In this study, the T1 relaxivity R of gadolinium-diethylene-triamine penta-acetic acid (Gd-DTPA) was measured following direct interstitial infusions of three different contrast agent concentrations to the parenchyma of rat brains. Changes in magnetic resonance (MR) T1 values were compared to brain slice concentrations determined with inductively coupled plasma atomic emission spectroscopy (ICP-AES) to determine R in 15 rats. Additionally, samples of cerebrospinal fluid, blood and urine were analyzed to evaluate possible Gd-DTPA clearance from the brain. The T1 relaxivity R of Gd-DTPA in the brain ECS was measured to be 5.35 (mM s){sup -1} in a 2.4 T field. This value is considerably higher than estimations used in studies by other groups. Measurements of brain Gd-DTPA tissue concentrations using MRI and ICP-AES demonstrated a high degree of coincidence. Clearance of Gd-DTPA was minimal at the time point immediately after infusion. These results suggest that the environment of the brain does in fact significantly affect Gd T1 relaxivity, and that MRI can accurately measure contrast agent concentrations when this relaxivity is well characterized.

  9. The diagnostic value of Gd-EOB-DTPA-MRI for the diagnosis of focal nodular hyperplasia: a systematic review and meta-analysis

    Energy Technology Data Exchange (ETDEWEB)

    Suh, Chong Hyun [University of Ulsan College of Medicine, Asan Medical Center, Department of Radiology and Research Institute of Radiology, 88 Olympic-ro 43-gil, Songpa-gu, Seoul (Korea, Republic of); Kim, Kyung Won; Shin, Yong Moon; Kim, Pyo Nyun [Asan Liver Center, Asan Medical Center, University of Ulsan College of Medicine, Seoul (Korea, Republic of); Kim, Gene Young [University of Missouri, School of Medicine, Columbia (United States); Park, Seong Ho [University of Ulsan College of Medicine, Asan Medical Center, Department of Radiology and Research Institute of Radiology, 88 Olympic-ro 43-gil, Songpa-gu, Seoul (Korea, Republic of); Asan Liver Center, Asan Medical Center, University of Ulsan College of Medicine, Seoul (Korea, Republic of)

    2015-04-01

    We aimed to systematically review the gadoxetic acid-enhanced magnetic resonance imaging (Gd-EOB-DTPA-MRI) findings of focal nodular hyperplasia (FNH) and its diagnostic value. A thorough literature search was conducted in Ovid-MEDLINE and EMBASE databases to identify studies evaluating Gd-EOB-DTPA-MRI findings of FNH. To evaluate the frequency of characteristic imaging findings on Gd-EOB-DTPA-MRI, pooled proportions of high/iso signal intensity (SI) on the hepatobiliary phase (HBP), arterial enhancement, high/iso SI on the portal-venous phase (PVP) or equilibrium phase (EP), and the central scar were calculated. Meta-analysis was performed to evaluate the diagnostic accuracy of high/iso SI on HBP for distinguishing FNH from hepatocellular adenoma. A review of 96 articles identified ten eligible articles with 304 patients with FNHs for meta-analysis. Pooled proportion of the Gd-EOB-DTPA-MRI findings showed that high/iso SI on the HBP, arterial enhancement, and high/iso SI on the PVP/EP were observed in 93% (95% CI, 90-97%), 99% (95% CI, 97-100%), and 97% (95% CI, 95-99%) of FNHs, respectively, while a central scar was observed in 61% of FNHs (95% CI, 47-74%). High/iso SI on the HBP was highly accurate for distinguishing FNH from hepatocellular adenoma, with a summary sensitivity of 93.9% (95% CI, 89.1-97.1%) and a specificity of 95.3% (95% CI, 88.4-98.7%). High/iso SI on the HBP of Gd-EOB-DTPA-MRI is characteristic and a prevalent finding of FNHs and can be helpful in the management of patients with FNH. (orig.)

  10. SYNTHESIS AND DOCKING STUDIES OF SCHIFF BASES DERIVED FROM 4-AMINOPYRIDINE

    Directory of Open Access Journals (Sweden)

    Jayita Nandi

    2012-10-01

    Full Text Available A Schiff base is a compound with a functional group that contains a carbon-nitrogen double bond with the nitrogen atom connected to aryl or alkyl group. In the present study, schiff bases were synthesized using 4-amino pyridine and different aldehydes, after assessing the drug likeliness properties. The structure of synthesised compounds were characterized by IR, NMR and Mass spectroscopic techniques. Docking studies were carried out for the schiff bases derived from 4-amino pyridine using Molegro Virtual Docker and protein beta-ketoacyl acyl carrier protein synthase II (mtKasB enzyme was selected as the target for M. tuberculosis. The results of docking study was compared with the standard drug isoniazid and the significant results were obtained.

  11. Deriving all minimal consistency-based diagnosis sets using SAT solvers

    Institute of Scientific and Technical Information of China (English)

    Xiangfu Zhao; Liming Zhang; Dantong Ouyang; Yu Jiao

    2009-01-01

    In this paper,a novel method is proposed for judging whether a component set is a consistency-based diagnostic set,using SAT solvers.Firstly,the model of the system to be diagnosed and all the observations are described with conjunctive normal forms (CNF).Then,all the related clauses in the CNF files to the components other than the considered ones are extracted,to be used for satisfiability checking by SAT solvers.Next,all the minimal consistency-based diagnostic sets are derived by the CSSE-tree or by other similar algorithms.We have implemented four related algorithms,by calling the gold medal SAT solver in SAT07 competition - RSAT.Experimental results show that all the minimal consistency-based diagnostic sets can be quickly computed.Especially our CSSE-tree has the best efficiency for the single-or double-fault diagnosis.

  12. Study of the Molecular Recognition of Nucleotides and Bases by a Novel Calixarene Derivative Containing Uracil

    Institute of Scientific and Technical Information of China (English)

    SHI,Hui-Jie; SHI,Xian-Fa; YAO,Tian-Ming; JI,Liang-Nian

    2008-01-01

    A calix[4]arene derivative containing uracil, 5-(uracil-N1-acetamido)-25,26,27,28-tetrahy droxycalix[4]-arene (UC), was designed and synthesized. The interaction with nucleotides and bases has also been studied by ESI-MS and π-A isotherms. The results of ESI-MS showed that UC could recognize adenine and adenosine from other nucleotides and bases. In addition, π-A isotherms at the air-water interface indicated that there was interaction between UC and the species in the subphase, and the respective complexes were formed in the monolayer. The mean molecular area at zero surface pressure increased with the sizes of the nucleotides and bases in the subphase in the order: water<adenine<adenosine<ATP·Na2.

  13. Derivative Trade Optimizing Model Utilizing GP Based on Behavioral Finance Theory

    Science.gov (United States)

    Matsumura, Koki; Kawamoto, Masaru

    This paper proposed a new technique which makes the strategy trees for the derivative (option) trading investment decision based on the behavioral finance theory and optimizes it using evolutionary computation, in order to achieve high profitability. The strategy tree uses a technical analysis based on a statistical, experienced technique for the investment decision. The trading model is represented by various technical indexes, and the strategy tree is optimized by the genetic programming(GP) which is one of the evolutionary computations. Moreover, this paper proposed a method using the prospect theory based on the behavioral finance theory to set psychological bias for profit and deficit and attempted to select the appropriate strike price of option for the higher investment efficiency. As a result, this technique produced a good result and found the effectiveness of this trading model by the optimized dealings strategy.

  14. Enhanced efficacy of combined {sup 213}Bi-DTPA-F3 and paclitaxel therapy of peritoneal carcinomatosis is mediated by enhanced induction of apoptosis and G2/M phase arrest

    Energy Technology Data Exchange (ETDEWEB)

    Vallon, Mario; Seidl, Christof; Blechert, Birgit; Li, Zhoulei; Gaertner, Florian C.; Senekowitsch-Schmidtke, Reingard; Essler, Markus [Technische Universitaet Muenchen, Department of Nuclear Medicine, Munich (Germany); Gilbertz, Klaus-Peter [German Armed Forces, Institute of Radiobiology, Munich (Germany); Baumgart, Anja [Technische Universitaet Muenchen, III. Medical Department, Munich (Germany); Aichler, Michaela; Feuchtinger, Annette; Walch, Axel K. [Helmholtz Zentrum Muenchen, Institute of Pathology, Neuherberg (Germany); Bruchertseifer, Frank; Morgenstern, Alfred [Institute for Transuranium Elements, European Commission, Joint Research Centre, Karlsruhe (Germany)

    2012-12-15

    Targeted therapy with {alpha}-particle emitting radionuclides is a promising new option in cancer therapy. Stable conjugates of the vascular tumour-homing peptide F3 with the {alpha}-emitter {sup 213}Bi specifically target tumour cells. The aim of our study was to determine efficacy of combined {sup 213}Bi-diethylenetriaminepentaacetic acid (DTPA)-F3 and paclitaxel treatment compared to treatment with either {sup 213}Bi-DTPA-F3 or paclitaxel both in vitro and in vivo. Cytotoxicity of treatment with {sup 213}Bi-DTPA-F3 and paclitaxel, alone or in combination, was assayed towards OVCAR-3 cells using the alamarBlue assay, the clonogenic assay and flow cytometric analyses of the mode of cell death and cell cycle arrest. Therapeutic efficacy of the different treatment options was assayed after repeated treatment of mice bearing intraperitoneal OVCAR-3 xenograft tumours. Therapy monitoring was performed by bioluminescence imaging and histopathologic analysis. Treatment of OVCAR-3 cells in vitro with combined {sup 213}Bi-DTPA-F3 and paclitaxel resulted in enhanced cytotoxicity, induction of apoptosis and G2/M phase arrest compared to treatment with either {sup 213}Bi-DTPA-F3 or paclitaxel. Accordingly, i.p. xenograft OVCAR-3 tumours showed the best response following repeated (six times) combined therapy with {sup 213}Bi-DTPA-F3 (1.85 MBq) and paclitaxel (120 {mu}g) as demonstrated by bioluminescence imaging and histopathologic investigation of tumour spread on the mesentery of the small and large intestine. Moreover, mean survival of xenograft mice that received combined therapy with {sup 213}Bi-DTPA-F3 and paclitaxel was significantly superior to mice treated with either {sup 213}Bi-DTPA-F3 or paclitaxel alone. Combined treatment with {sup 213}Bi-DTPA-F3 and paclitaxel significantly increased mean survival of mice with peritoneal carcinomatosis of ovarian origin, thus favouring future therapeutic application. (orig.)

  15. Safety, immunogenicity and persistence of immune response to the combined diphtheria, tetanus, acellular pertussis, poliovirus and Haemophilus influenzae type b conjugate vaccine (DTPa-IPV/Hib) administered in Chinese infants

    Science.gov (United States)

    Li, Yanping; Li, Rong Cheng; Ye, Qiang; Li, Changgui; Liu, You Ping; Ma, Xiao; Li, Yanan; Zhao, Hong; Chen, Xiaoling; Assudani, Deepak; Karkada, Naveen; Han, Htay Htay; Van Der Meeren, Olivier; Mesaros, Narcisa

    2017-01-01

    ABSTRACT We conducted 3 phase III, randomized, open-label, clinical trials assessing the safety, reactogenicity (all studies), immunogenicity (Primary vaccination study) and persistence of immune responses (Booster study) to the combined diphtheria, tetanus, pertussis, poliomyelitis, and Haemophilus influenzae type b vaccine (DTPa-IPV/Hib) in Chinese infants and toddlers. In the Pilot study (NCT00964028), 50 infants (randomized 1:1) received 3 doses of DTPa-IPV/Hib at 2–3–4 (Group A) or 3–4–5 months of age (Group B). In the Primary study (NCT01086423), 984 healthy infants (randomized 1:1:1) received 3 doses of DTPa-IPV/Hib at 2–3–4 (Group A) or 3–4–5 (Group B) months of age, or concomitant DTPa/Hib and poliomyelitis (IPV) vaccination at 2–3–4 months of age (Control group); 825 infants received a booster dose of DTPa/Hib and IPV at 18–24 months of age (Booster study; NCT01449812). In the Pilot study, unsolicited symptoms were more frequent in Group A (16 versus 1 infant; mostly upper respiratory tract infection and pyrexia); this observation was attributed to an epidemic outbreak of viral infections. Non-inferiority of 3-dose primary vaccination with DTPa-IPV/Hib over separately administered DTPa/Hib and IPV was demonstrated for Group A (primary objective). Similar antibody concentrations were observed in all groups, except for anti-polyribosyl-ribitol phosphate and anti-poliovirus types 1–3 which were higher in DTPa-IPV/Hib recipients. Protective antibody levels against all vaccine antigens remained high until booster vaccination. Three-dose vaccination with DTPa-IPV/Hib had a clinically acceptable safety profile. PMID:27768515

  16. Metal-based biologically active azoles and β-lactams derived from sulfa drugs.

    Science.gov (United States)

    Ebrahimi, Hossein Pasha; Hadi, Jabbar S; Almayah, Abdulelah A; Bolandnazar, Zeinab; Swadi, Ali G; Ebrahimi, Amirpasha

    2016-03-01

    Metal complexes of Schiff bases derived from sulfamethoxazole (SMZ) and sulfathiazole (STZ), converted to their β-lactam derivatives have been synthesized and experimentally characterized by elemental analysis, spectral (IR, (1)H NMR, (13)C NMR, and EI-mass), molar conductance measurements and thermal analysis techniques. The structural and electronic properties of the studied molecules were investigated theoretically by performing density functional theory (DFT) to access reliable results to the experimental values. The spectral and thermal analysis reveals that the Schiff bases act as bidentate ligands via the coordination of azomethine nitrogen to metal ions as well as the proton displacement from the phenolic group through the metal ions; therefore, Cu complexes can attain the square planner arrangement and Zn complexes have a distorted tetrahedral structure. The thermogravimetric (TG/DTG) analyses confirm high stability for all complexes followed by thermal decomposition in different steps. In addition, the antibacterial activities of synthesized compounds have been screened in vitro against various pathogenic bacterial species. Inspection of the results revealed that all newly synthesized complexes individually exhibit varying degrees of inhibitory effects on the growth of the tested bacterial species, therefore, they may be considered as drug candidates for bacterial pathogens. The free Schiff base ligands (1-2) exhibited a broad spectrum antibacterial activity against Gram negative Escherichia coli, Pseudomonas aeruginosa, and Proteus spp., and Gram positive Staphylococcus aureus bacterial strains. The results also indicated that the β-lactam derivatives (3-4) have high antibacterial activities on Gram positive bacteria as well as the metal complexes (5-8), particularly Zn complexes, have a significant activity against all Gram negative bacterial strains. It has been shown that the metal complexes have significantly higher activity than corresponding

  17. Perspectives for Titanium-Derived Fillers Usage on Denture Base Composite Construction: A Review Article

    Directory of Open Access Journals (Sweden)

    Nidal W. Elshereksi

    2014-01-01

    Full Text Available Poly(methyl methacrylate (PMMA is an extensively used material in dentistry because of its aesthetics, processability, and reparability. However, PMMA is still far from being ideal in fulfilling the mechanical requirements of prosthesis. PMMA-based denture base polymers exhibit low fracture resistance and radiopacity behavior. Efforts to improve the mechanical and radiopacity properties of denture base materials through inclusion of silica-based fillers are ongoing. Although silane-treated siliceous fillers are commonly used, they are not sufficiently strong. They also exhibit cracks, which either cut through the glass fillers or propagate around the filler particles. This defect occurs when the dental composites are placed in aqueous oral environment because of the hydrolytic degradation of silica-based fillers and silane-coupling agents. The clinical problem of using silanes in adhesion promotion is bond degradation over time in oral environment. In addition, silanes do not bond effectively to nonsilica-based dental restorative materials. This review presents titanium-derived fillers as alternatives to siliceous fillers. Titanate-coupling agents are found to be effective couplers in treating Ti-based fillers because of their chemical compatibility and relatively high stability in aqueous environment.

  18. Novel 2DG-based harmine derivatives for targeted cancer therapy

    Science.gov (United States)

    Wang, Aqin; Chen, Yuqi; Chen, Wei R.; Gu, Yueqing

    2013-02-01

    Harmine is a beta-carboline alkaloid from the plant Peganum harmala. These alkaloids were stimulated by their promising antitumor activities in the recent years. In this study, we designed and synthesized two harmine derivatives #1and #2 modified at position-9 of harmine with ethyl and phenylpropyl, respectively. To improve the tumor targeting capability, #1' and #2' were synthesized by conjugating 2-amino-2-deoxy-D-glucose (2DG) to the derivatives #1 and #2, respectively. The MTT assays of all these compounds in vitro against L02, HepG2 showed all compounds had low toxicity to normal cells (L02) and significantly enhanced carcinoma cell inhibitory rate compared to harmine. Cytotoxicity against liver cancer cell lines of compound #1' #2' is higher than #1 #2, and even the compound #2' is better than positive drug 5-FU. The compound #2', a novel 2DG-based harmine derivatives, could become a promising drug for targeted cancer therapy and combination therapy with other antitumor drugs.

  19. Evaluation of deriving fire cycle of forested landscape based on time-since-fire distribution

    Institute of Scientific and Technical Information of China (English)

    ZHANG Quan-fa; Kurt S. Pregitzer; JIANG Ming-xi; CHEN Wen-jun

    2006-01-01

    Estimation of fire cycle has been conducted by using the negative exponential function as an approximation of time-since-fire distribution of a landscape assumed .to be homogeneous with respect to fire spread processes. The authors imposed predefined fire cycles on a virtual landscape of 100 cell ×100 cell, and obtained a mosaic composing of patches with different stand ages (i.e. time since fire). Graphical and statistical methods (Van Wagner 1978; Reed et al. 1998) were employed to derive fire cycle from the virtual landscape. By comparing the predefined and the derived fire cycles, the two methods and tested the effects of sample size and hazard of burning (i.e., stand's susceptibility to fire in relation to its stand age) were evaluated on fire cycle deviation. The simulation results indicated a minimum sample size of 10 times of the annual burnt area would be required for partitioning time-since-fire distribution into homogeneous epochs indicating temporal change in fire cycle. Statistically, there was significant difference among the imposed and the derived fire cycle, regardless of sample sizes with or without consideration of hazard of burning. Both methods underestimated the more recent fire cycle without significant difference between them. The results imply that deviation of fire cycle based on time-since-fire distribution warrants cautious interpretation, especially when a landscape is spatially partitioned into small units and temporal changes in fire cycle are involved.

  20. Deformation analysis of polymers composites: rheological model involving time-based fractional derivative

    Science.gov (United States)

    Zhou, H. W.; Yi, H. Y.; Mishnaevsky, L.; Wang, R.; Duan, Z. Q.; Chen, Q.

    2016-08-01

    A modeling approach to time-dependent property of Glass Fiber Reinforced Polymers (GFRP) composites is of special interest for quantitative description of long-term behavior. An electronic creep machine is employed to investigate the time-dependent deformation of four specimens of dog-bond-shaped GFRP composites at various stress level. A negative exponent function based on structural changes is introduced to describe the damage evolution of material properties in the process of creep test. Accordingly, a new creep constitutive equation, referred to fractional derivative Maxwell model, is suggested to characterize the time-dependent behavior of GFRP composites by replacing Newtonian dashpot with the Abel dashpot in the classical Maxwell model. The analytic solution for the fractional derivative Maxwell model is given and the relative parameters are determined. The results estimated by the fractional derivative Maxwell model proposed in the paper are in a good agreement with the experimental data. It is shown that the new creep constitutive model proposed in the paper needs few parameters to represent various time-dependent behaviors.

  1. A derives grid-based model for simulation of pedestrian flow

    Institute of Scientific and Technical Information of China (English)

    Min-jie CHEN; Günter B(A)RWOLFF; Hartmut SCHWANDT

    2009-01-01

    We present a derived grid-based model for the simulation of pedestrian flow. Interactions among pedestrians are considered as the result of forces within a certain neighbourhood. Unlike the social force model, the forces here, as in Newtonian physics, are proportional to the inverse of the square of the distance. Despite the notion of neighbourhood and the underlying grid, this model differs from the existing cellular automaton (CA) models in that the pedestrians are treated as individuals. Bresenham's algorithm for line rastering is applied in the step calculation.

  2. Electrochemical Biosensors Based on Ferroceneboronic Acid and Its Derivatives: A Review

    Directory of Open Access Journals (Sweden)

    Baozhen Wang

    2014-07-01

    Full Text Available We review recent progress in the development of electrochemical biosensors based on ferroceneboronic acid (FcBA and ferrocene (Fc-modified boronic acids. These compounds can be used to construct electrochemical biosensors because they consist of a binding site (i.e., a boronic acid moiety and an electrochemically active part (i.e., an Fc residue. By taking advantage of the unique properties of FcBA and its derivatives, electrochemical sensors sensitive to sugars, glycated hemoglobin (HbA1c, fluoride (F− ions, and so forth have been widely studied. FcBA-based sugar sensors rely on the selective binding of FcBA to 1,2- or 1,3-diol residues of sugars through the formation of cyclic boronate ester bonds. The redox properties of FcBA-sugar adduct differ from those of free FcBA, which forms the basis of the electrochemical determination of sugars. Thus, non-enzymatic glucose sensors are now being actively studied using FcBA and Fc-modified boronic acids as redox markers. Using a similar principle, HbA1c can be detected by FcBA-based electrochemical systems because it contains hydrocarbon chains on the polypeptide chain. HbA1c sensors are useful for monitoring blood glucose levels over the preceding 8–12 weeks. In addition, FcBA and Fc-modified boronic acids have been used for the detection of F− ions due to the selective binding of boronic acid to F− ions. F−-ion sensors may be useful alternatives to conventional ion-selective electrodes sensitive to F− ion. Furthermore, FcBA derivatives have been studied to construct lectin; steroids; nucleotides; salicylic acid; and bacteria sensors. One of the limitations of FcBA-based sensors comes from the fact that FcBA derivatives are added in sample solutions as reagents. FcBA derivatives should be immobilized on the surface of electrodes for developing reagentless sensors.

  3. Integral Theorems Based on a New Gradient Operator Derived from Biomembranes (Part Ⅱ): Applications

    Institute of Scientific and Technical Information of China (English)

    YIN Yajun

    2005-01-01

    Based on the second gradient operator and corresponding integral theorems such as the second divergence theorem, the second gradient theorem, the second curl theorem, and the second circulation theorem on curved surfaces, a few new scalar differential operators are defined and a series of integral transformations are derived. Interesting transformations between the average curvature and the Gauss curvature are presented. Various conserved integrals related to the Gauss curvature and the second fundamental tensor are disclosed. The important applications of the results in disciplines such as the geometry, physics, mechanics, and biology are briefly discussed.

  4. Development of Ni-Based Catalysts for Steam Reforming of Tar Derived from Biomass Pyrolysis

    Institute of Scientific and Technical Information of China (English)

    Dalin LI; Yoshinao NAKAGAWA; Keiichi TOMISHIGE

    2012-01-01

    Nickel catalysts are effective for the steam reforming of tar derived from biomass pyrolysis,but the improvement is needed in terms of activity,stability,suppression of coke deposition and aggregation,and regeneration.Our recent development of Ni-based catalysts for the steam reforming of tar is reviewed including the modification with CeO2 (or MnO),trace Pt,and MgO.The role of additives such as CeO2,MnO,Pt,and MgO is also discussed.

  5. Synthesis and antimalarial evaluation of some 4-quinazolinone derivatives based on febrifugine

    Directory of Open Access Journals (Sweden)

    Debanjan Sen

    2010-01-01

    Full Text Available A series of 2-substituted and 2,3-substituted quinazolin -4(3H-one derivatives were designed and synthesized based on the structure of febrifugine. The structures of the new compounds were confirmed by spectral analysis. The in vivo biological activity test results indicated that those compounds exhibited antimalarial activities against Plasmodium berghei in mice, at a dose of 5 mg/kg. Compared to Chloroquine and Artemisinin, these compounds have the advantages of shorter synthetic routes and consequently are highly cost effective in nature.

  6. An efficient nonviral gene-delivery vector based on hyperbranched cationic glycogen derivatives

    Directory of Open Access Journals (Sweden)

    Liang X

    2014-01-01

    Full Text Available Xuan Liang,1,* Xianyue Ren,2,* Zhenzhen Liu,1 Yingliang Liu,1 Jue Wang,2 Jingnan Wang,2 Li-Ming Zhang,1 David YB Deng,2 Daping Quan,1 Liqun Yang1 1Institute of Polymer Science, School of Chemistry and Chemical Engineering, Key Laboratory of Designed Synthesis and Application of Polymer Material, Key Laboratory for Polymeric Composite and Functional Materials of Ministry of Education, Sun Yat-Sen University, Guangzhou, People's Republic of China; 2Research Center of Translational Medicine, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou, People's Republic of China *Both these authors contributed equally to this work Background: The purpose of this study was to synthesize and evaluate hyperbranched cationic glycogen derivatives as an efficient nonviral gene-delivery vector. Methods: A series of hyperbranched cationic glycogen derivatives conjugated with 3-(dimethylamino-1-propylamine (DMAPA-Glyp and 1-(2-aminoethyl piperazine (AEPZ-Glyp residues were synthesized and characterized by Fourier-transform infrared and hydrogen-1 nuclear magnetic resonance spectroscopy. Their buffer capacity was assessed by acid–base titration in aqueous NaCl solution. Plasmid deoxyribonucleic acid (pDNA condensation ability and protection against DNase I degradation of the glycogen derivatives were assessed using agarose gel electrophoresis. The zeta potentials and particle sizes of the glycogen derivative/pDNA complexes were measured, and the images of the complexes were observed using atomic force microscopy. Blood compatibility and cytotoxicity were evaluated by hemolysis assay and MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide assay, respectively. pDNA transfection efficiency mediated by the cationic glycogen derivatives was evaluated by flow cytometry and fluorescence microscopy in the 293T (human embryonic kidney and the CNE2 (human nasopharyngeal carcinoma cell lines. In vivo delivery of pDNA in model animals (Sprague Dawley

  7. Real-Time Algebraic Derivative Estimations Using a Novel Low-Cost Architecture Based on Reconfigurable Logic

    OpenAIRE

    2014-01-01

    Time derivative estimation of signals plays a very important role in several fields, such as signal processing and control engineering, just to name a few of them. For that purpose, a non-asymptotic algebraic procedure for the approximate estimation of the system states is used in this work. The method is based on results from differential algebra and furnishes some general formulae for the time derivatives of a measurable signal in which two algebraic derivative estimators run simultaneously...

  8. Alveolar epithelial permeability in bronchial asthma in children; An evaluation by [sup 99m]Tc-DTPA inhalation scintigraphy

    Energy Technology Data Exchange (ETDEWEB)

    Oishi, Takuji (Nippon Medical School, Tokyo (Japan))

    1993-02-01

    To evaluate alveolar epithelial permeability (k[sub ep]) in children with bronchial asthma, [sup 99m]Tc-DTPA (diethylene triamine penta acetate) aerosol lung inhalation scintigraphies were performed. There was no correlation between the k[sub ep] value and the severity of asthma. On the other hand, out of 10 cases which had no aerosol deposition defect in the lung field, 4 showed high k[sub ep] values on the whole lung field and 7 had high k[sub ep] value areas, particularly apparent in the upper lung field. These results suggest that even when the central airway lesions are mild, severe damage exists in the alveolar region of the peripheral airway. (author).

  9. Investigation of the usefulness of NTA, EDTA and DTPA in separation of some platinum metals on cellulose exchangers.

    Science.gov (United States)

    Brajter, K; Słonawska, K

    1980-09-01

    The possibility of using NTA, EDTA and DTPA as complexing agents for separation of some platinum group ions on cellulose ion-exchangers has been investigated. The greatest differences in the affinities of Pd(II) and Pt(IV) toward the cellulose ion-exchangers are obtained in the presence of DPTA, Cellex D (as ion-exchanger) in hydroxide form. The column separation of Pd(II) from Pt(IV), Rh(III) from Pd(II) and of a Rh(III)Pd(II)Pt(IV) mixture can be achieved with DPTA and chloride solutions. The method can be for determination of the components of RhPdPt alloys.

  10. Capillary transfer constant of Gd-DTPA in the myocardium at rest and during vasodilation assessed by MRI

    DEFF Research Database (Denmark)

    Fritz-Hansen, T; Rostrup, Egill; Søndergaard, Lise;

    1998-01-01

    The purpose of this study was to determine whether the capillary transfer constant (Ki) of gadolinium-DTPA was sensitive to perfusion changes and whether ischemic regions in the myocardium could be identified using the modified Kety formula. Ki was measured at rest and during dipyridamole......-induced vasodilation in 10 healthy volunteers and in 10 patients with ischemic heart disease. Ki increased by a factor of 2.5+/-1.2 (mean +/- SD) from 55+/-16 ml 100 g(-1)min(-1) at rest to 136+/-46 ml 100 g(-1)min(-1) (P ... during vasodilation in ischemic regions (50+/-18 versus 49+/-30 ml 100 g(-1)min(-1) (P > 0.4)). Ki increased in nonischemic regions by a factor of 2.0+/-0.8 from 44+/-17 to 81+/-32 ml 100 g(-1)min(-1) during vasodilation (P transfer constant is sensitive...

  11. Schistosomiasis haematobia in Brazilian patients: clinical and renal functional evaluation with {sup 99m}Tc-DTPA

    Energy Technology Data Exchange (ETDEWEB)

    DAlmeida, J.; Maliska, C.I.; Pellegrini, P.M.; Collares, R.A.I [Army Central Hospital, Rio de Janeiro, RJ (Brazil). Nuclear Medicine Service]. E-mail: joaquimjdalmeida@yahoo.com.br; Penas, M.E.; Cruz, M.G.A. [Clementino Fraga Filho School Hospital, (UFRJ), Rio de Janeiro, RJ (Brazil). Nuclear Medicine Service

    2007-07-01

    The present study was carried out at the Army Central Hospital, Rio de Janeiro, Brazil, from September 2000 to December 2001, employing diethylenetriamine penta-acetic acid labeled with technetium-99m ({sup 99m}Tc-DTPA) to evaluate the renal function of nineteen symptomatic patients infected with S. haematobium during a peace mission in Mozambique. Results evidenced that the most frequent clinical manifestations were hematuria (68.4%) and low back pain (68.4%) and 73.7% patients had altered dynamic renal scintigraphy expressed by an increase in the excretory phase independently of the symptoms duration; furthermore, none of them had mechanical obstructive pattern. Schistosoma haematobium glomerulopathy could be considered a pathological finding without correlation with the disease clinical manifestations. (author)

  12. In vivo evaluation of {sup 111}In-DTPA-N-TIMP-2 in Kaposi sarcoma associated with HIV infection

    Energy Technology Data Exchange (ETDEWEB)

    Kulasegaram, R.; Peters, B.S. [Academic Dept. of STD' S, Guys and St Thomas' Hospital, London (United Kingdom); Giersing, B.; Williamson, R.A. [Biosciences Dept., Kent Univ., Canterbury (United Kingdom); Page, C.J. [Dept. of Nuclear Medicine, Guys and St. Thomas' Hospital, London (United Kingdom); Blower, P.J. [Biosciences Dept., Kent Univ., Canterbury (United Kingdom); Dept. of Nuclear Medicine, Kent and Canterbury Hospital, Canterbury (United Kingdom); O' Doherty, M.J. [Dept. of Nuclear Medicine, Kent and Canterbury Hospital, Canterbury (United Kingdom); Dept. of Nuclear Medicine, Guys and St. Thomas' Hospital, London (United Kingdom)

    2001-06-01

    Matrix metalloproteinases are the major agents responsible for the degradation of extracellular matrix and are produced at high levels by transformed and tumour cells, where they participate in the metastatic process by allowing local invasion. They are also more active at sites of new normal growth and angiogenesis. In the early stages of Kaposi sarcoma (KS), in vitro studies have demonstrated that vascular invasion can be inhibited by inhibitors of matrix metalloproteinases. Imaging of visceral and cutaneous KS presents a problem and therefore the potential use of a labelled inhibitor of metalloproteinases, N-TIMP-2, with indium-111 was thought to present a possible imaging tool. The biokinetics, dosimetry and potential for imaging with {sup 111}In-DTPA-N-TIMP-2 were assessed in five patients with HIV infection and KS. Between 103.1 and 108.0 MBq of this agent was injected into each patient, and the dynamic uptake over the kidneys was assessed, whole body scans were performed and blood samples were obtained. The clearance from the blood was rapid, with a first component half-time of 16.6{+-}3.4 min and a second component half-time of 9.68{+-}2.68 h. Two out of five patients experienced minor shivering but one of these patients was generally unwell before the study. The last three patients had no such problems. The tracer distributed predominantly to the kidneys and did not localise in other tissues. No KS lesions were clearly identified. {sup 111}In-DTPA-N-TIMP-2 can be successfully prepared and administered to patients safely, with a biodistribution and dosimetry which would allow its use as an imaging tracer. It is unlikely to be of use for imaging KS, but may have a role in other tumours that produce matrix metalloproteinases. (orig.)

  13. Baseline Tc-99m DTPA renal scintigraphy as a predictor of outcome in children with urinary tract infection

    Energy Technology Data Exchange (ETDEWEB)

    Yun, J. G.; An, Y. S.; Lee, M. H.; Cho, C. W.; Yun, S. N.; Pai, G. S [Ajou University Medical Center, Suwon (Korea, Republic of)

    2004-07-01

    Tc-99m DTPA renal scintigraphy is useful in detecting urinary tract obstruction in patients with urinary tract infection (UTI). We evaluated the prognostic significance of baseline Tc-99m DTPA renal scintigraphy in children with UTI. Among children, who underwent both baseline/follow-up Tc-99m DMSA scintigraphies and baseline Tc-99m DTP A scintigraphy for evaluation of UTI, 32 patients with unilateral cortical defects on baseline Tc-99m DMSA scintigraphy were included in the study. The outcome of cortical defects was evaluated on follow-up Tc-99m DMSA scintigraphy by visual analysis. ROIs were drawn on the Tc-99m DPTA scintigraphy for calculation of ipsilateral to contralateral kidney ratio (ICR) at blood flow phase (< 60s, BFP) and cortical uptake phase (1-5 min, CUP). Median follow-up period of Tc-99m DMSA scintigraphy was 3.2 months (1.4 - 14 months). There were 24 patients with healing cortical defects and 8 with cortical scarring. Average ICRs of patients with healing defects were 1.11 {+-} 0.18 (0.44 - 1.57) at BFP and 0.97 {+-} 0.21 (0.31 - 1.28) at CUP, while those of patients with cortical scarring were 0.97 {+-} 0.47 (0.21 - 0.89) at BFP and 0.75 {+-} 0.49 (0.19 - 1.65) at CUP. ICR more than 0.9 was determined as a good prognostic indicator. The sensitivity, specificity, positive predictive value, and negative predictive value of ICR was 83.3%, 100%, 100% and 66.7% at BFP, and 79.0%, 62.5%, 86.4% and 50.0% at CUP. Ipsilateral to contralateral kidney ratio on baseline Tc-99m DPTA renal scintigraphy, especially at blood flow phase, is helpful in predicting outcome of children with UTI.

  14. In vivo Gd-DTPA concentration for MR lung perfusion measurements: Assessment with computed tomography in a porcine model

    Energy Technology Data Exchange (ETDEWEB)

    Puderbach, Michael [German Cancer Research Center, Department of Radiology, Heidelberg (Germany); Deutsches Krebsforschungszentrum, Department of Radiology (E010), Heidelberg (Germany); Risse, Frank; Semmler, Wolfhard [German Cancer Research Center, Department of Medical Physics in Radiology, Heidelberg (Germany); Biederer, Juergen [University Hospital Schleswig-Holstein, Department of Diagnostic Radiology, Campus Kiel (Germany); Ley-Zaporozhan, Julia; Ley, Sebastian [German Cancer Research Center, Department of Radiology, Heidelberg (Germany); University Heidelberg, Department of Pediatric Radiology, Heidelberg (Germany); Szabo, Gabor [University Heidelberg, Department of Cardiac Surgery, Heidelberg (Germany); Kauczor, Hans-Ulrich [University of Heidelberg, Department of Radiology, Heidelberg (Germany)

    2008-10-15

    A linear relationship between MR signal and contrast-agent concentration (CAC) of the arterial-input function (AIF) is crucial for MR lung-perfusion quantification. The aim was to determine the in-vivo real maximum CAC of the AIF, using cine CT measurements in a porcine model. A dilution series (Gd-DTPA, 0-20 mM) was examined by clinical time-resolved 3D-GRE MRI and by MDCT in cine CT mode. Using the CT setup, data were acquired in five pigs immediately after the injection of 0.05 mmol and 0.07 mmol/kg BW Gd-DTPA. For phantom measurements, mean signal values were determined using a region-of-interest (ROI) analysis and for animal measurements, a ROI was placed in the pulmonary trunk of the cine CT perfusion data sets. The CT phantom measurements were used to calculate the in-vivo maximum CAC corresponding to the HU values obtained in the pulmonary trunk by the cine CT study. Linearity of the AIF of the CT perfusion measurements was verified using the MR phantom measurement results. MR phantom measurements demonstrated linearity for concentrations of 0-4 mM. CT phantom measurements showed linear relation for the entire CAC range. Comparing in-vivo and in-vitro measurements, three of five CA injections at 0.05 mmol/kg and all 0.07 mmol/kg injections exceeded the range of linearity in MRI. The CA dose for quantification of lung perfusion with time-resolved MR studies must be chosen carefully since even with low doses (0.05 mmol/kg) the CAC may exceed the range of linearity in the AIF. (orig.)

  15. Emitting materials based on phenylanthracene-substituted naphthalene derivatives for organic light-emitting diodes

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Hyun Woo; Kim, Hye Jeong; Kim, Young Seok; Kim, Jwajin [Department of Chemistry, Sungkyunkwan University, Suwon 440‐746 (Korea, Republic of); Lee, Song Eun; Lee, Ho Won [Department of Information Display, Hongik University, Seoul 121-791 (Korea, Republic of); Kim, Young Kwan, E-mail: kimyk@wow.hongik.ac.kr [Department of Information Display, Hongik University, Seoul 121-791 (Korea, Republic of); Yoon, Seung Soo, E-mail: ssyoon@skku.edu [Department of Chemistry, Sungkyunkwan University, Suwon 440‐746 (Korea, Republic of)

    2015-09-15

    This study reports the emitting materials based on phenylanthracene-substituted naphthalene derivatives to achieve efficient electroluminescent properties for OLED applications. An OLED device using 4,4′-bis(10-phenylanthracen-9-yl)-1,1′-binaphthalene exhibited the blue emission with the CIE coordinates of (0.19, 0.16) and efficient electroluminescent properties with the luminance, power and external quantum efficiency of 1.70 cd/A, 0.79 lm/W and 1.26% at 20 mA/cm{sup 2}, respectively. Also, the other device using 1,4-bis(10-phenylanthracene-9-yl)naphthalene exhibited white emission with the CIE coordinates of (0.34, 0.43) at 7V, respectively. This device exhibits the luminance, power and external quantum efficiency of 2.22 cd/A, 1.13 lm/W and 0.86% at 20 mA/cm{sup 2}, respectively. - Highlights: • We synthesized fluorescent materials based on phenylanthracene derivatives. • Electroluminescence properties of these materials depend on the molecular structures. • These blue and white materials have great potential for application in OLEDs.

  16. Potential Biological Applications of Bio-Based Anacardic Acids and Their Derivatives

    Directory of Open Access Journals (Sweden)

    Fatma B. Hamad

    2015-04-01

    Full Text Available Cashew nut shells (CNS, which are agro wastes from cashew nut processing factories, have proven to be among the most versatile bio-based renewable materials in the search for functional materials and chemicals from renewable resources. CNS are produced in the cashew nut processing process as waste, but they contain cashew nut shell liquid (CNSL up to about 30–35 wt. % of the nut shell weight depending on the method of extraction. CNSL is a mixture of anacardic acid, cardanol, cardol, and methyl cardol, and the structures of these phenols offer opportunities for the development of diverse products. For anacardic acid, the combination of phenolic, carboxylic, and a 15-carbon alkyl side chain functional group makes it attractive in biological applications or as a synthon for the synthesis of a multitude of bioactive compounds. Anacardic acid, which is about 65% of a CNSL mixture, can be extracted from the agro waste. This shows that CNS waste can be used to extract useful chemicals and thus provide alternative green sources of chemicals, apart from relying only on the otherwise declining petroleum based sources. This paper reviews the potential of anacardic acids and their semi-synthetic derivatives for antibacterial, antitumor, and antioxidant activities. The review focuses on natural anacardic acids from CNS and other plants and their semi-synthetic derivatives as possible lead compounds in medicine. In addition, the use of anacardic acid as a starting material for the synthesis of various biologically active compounds and complexes is reported.

  17. Potential biological applications of bio-based anacardic acids and their derivatives.

    Science.gov (United States)

    Hamad, Fatma B; Mubofu, Egid B

    2015-04-16

    Cashew nut shells (CNS), which are agro wastes from cashew nut processing factories, have proven to be among the most versatile bio-based renewable materials in the search for functional materials and chemicals from renewable resources. CNS are produced in the cashew nut processing process as waste, but they contain cashew nut shell liquid (CNSL) up to about 30-35 wt. % of the nut shell weight depending on the method of extraction. CNSL is a mixture of anacardic acid, cardanol, cardol, and methyl cardol, and the structures of these phenols offer opportunities for the development of diverse products. For anacardic acid, the combination of phenolic, carboxylic, and a 15-carbon alkyl side chain functional group makes it attractive in biological applications or as a synthon for the synthesis of a multitude of bioactive compounds. Anacardic acid, which is about 65% of a CNSL mixture, can be extracted from the agro waste. This shows that CNS waste can be used to extract useful chemicals and thus provide alternative green sources of chemicals, apart from relying only on the otherwise declining petroleum based sources. This paper reviews the potential of anacardic acids and their semi-synthetic derivatives for antibacterial, antitumor, and antioxidant activities. The review focuses on natural anacardic acids from CNS and other plants and their semi-synthetic derivatives as possible lead compounds in medicine. In addition, the use of anacardic acid as a starting material for the synthesis of various biologically active compounds and complexes is reported.

  18. Characterization of ι-carrageenan and its derivative based green polymer electrolytes

    Energy Technology Data Exchange (ETDEWEB)

    Jumaah, Fatihah Najirah; Mobaraka, Nadhratun Naiim; Ahmad, Azizan; Ramli, Nazaruddin [School of Chemical Sciences and Food Technology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600, Bangi, Selangor Darul Ehsan (Malaysia)

    2013-11-27

    The new types of green polymer electrolytes based on ι-carrageenan derivative have been prepared. ι-carrageenan act as precursor was reacted with monochloroacetic acid to produce carboxymethyl ι-carrageenan. The powders were characterized by Attenuated Total Reflection Fourier Transform infrared (ATR-FTIR) spectroscopy and {sup 1}H nuclear magnetic resonance (NMR) to confirm the substitution of targeted functional group in ι-carrageenan. The green polymer electrolyte based on ι-carrageenan and carboxymethyl ι-carrageenan was prepared by solution-casting technique. The films were characterized by electrochemical impedance spectroscopy to determine the ionic conductivity. The ionic conductivity ι-carrageenan film were higher than carboxymethyl ι-carrageenan which 4.87 ×10{sup −6} S cm{sup −1} and 2.19 ×10{sup −8} S cm{sup −1}, respectively.

  19. Cell-based delivery of brain-derived neurotrophic factor in experimental allergic encephalomyelitis.

    Science.gov (United States)

    Makar, Tapas K; Nimmagadda, Vamshi K C; Trisler, David; Bever, Christopher T

    2014-08-01

    Brain-derived neurotrophic factor (BDNF) is a pleiotropic cytokine with neuroprotective properties that has been identified as a potential therapeutic agent for diseases of the central nervous system (CNS). The use of BDNF has been limited by a short serum half-life and poor penetration of the blood-brain barrier. To address this limitation we have explored cell-based approaches to delivery. We have used experimental allergic encephalomyelitis (EAE), an inflammatory disease of the CNS, as a model system. We engineered hematopoietic stem cells to produce BDNF to determine the feasibility and effectiveness of cell-based delivery of BDNF into the CNS in EAE. We review those studies here.

  20. Decomposition driven interface evolution for layers of binary mixtures: I. Model derivation and stratified base states

    CERN Document Server

    Thiele, Uwe; Frastia, Lubor

    2007-01-01

    A dynamical model is proposed to describe the coupled decomposition and profile evolution of a free surface film of a binary mixture. An example is a thin film of a polymer blend on a solid substrate undergoing simultaneous phase separation and dewetting. The model is based on model-H describing the coupled transport of the mass of one component (convective Cahn-Hilliard equation) and momentum (Navier-Stokes-Korteweg equations) supplemented by appropriate boundary conditions at the solid substrate and the free surface. General transport equations are derived using phenomenological non-equilibrium thermodynamics for a general non-isothermal setting taking into account Soret and Dufour effects and interfacial viscosity for the internal diffuse interface between the two components. Focusing on an isothermal setting the resulting model is compared to literature results and its base states corresponding to homogeneous or vertically stratified flat layers are analysed.

  1. Soot particle sizing based on analytical formula derived from laser-induced incandescence decay signals

    Science.gov (United States)

    Wu, Jian; Chen, Linghong; Yan, Mingming; Wu, Xuecheng; Gréhan, Gérard; Cen, Kefa

    2017-01-01

    The laser-induced incandescence (LII) signal during a heat-conduction-dominated cooling process was used to derive an analytical formula to describe the relationship between the soot particle size and the LII signal decay time by exponential fitting. The formula was used to determine particle sizes based on the experimental LII signals at different detection wavelengths for an atmospheric C2H4/air diffusion flame. The results agree with those obtained from temporal temperature measurements. The measurements and numerical calculations demonstrate that particle sizing depends weakly on the maximum temperature in the formula within a typical heat-up temperature range. The results show that based on this formula, a compact single-color LII detection system can be used for particle sizing with low uncertainty under most practical combustion conditions, at least in cases where heat conduction is dominant and occurs in a free molecular regime during particle cooling.

  2. Liquid chromatographic determination of beta-cyclodextrin derivatives based on fluorescence enhancement after inclusion complexation.

    Science.gov (United States)

    Reeuwijk, H J; Irth, H; Tjaden, U R; Merkus, F W; van der Greef, J

    1993-04-21

    A liquid chromatographic method using fluorescence detection for the determination of beta-cyclodextrin (beta CD) and its derivatives is presented. The chromatographic system is based on size-exclusion chromatography with the addition of the fluorophoric compound 1-naphthol to the mobile phase. Detection is based on fluorescence enhancement caused by the formation of inclusion complexes. By incorporating 10(-4) M 1-naphthol in the mobile phase, detection limits of 90, 27, 370 and 37 pmol were obtained for beta CD, hydroxypropyl-beta CD, trimethyl-beta CD and dimethyl-beta CD, respectively. The method was applied to the determination of dimethyl-beta CD in urine: the minimum detectable concentration was 0.2 microgram/ml after preconcentration of 10 ml of urine.

  3. Spectral Characterization and Antimicrobial Activity of Some Schiff Bases Derived from 4-Chloro-2-aminophenol and Various Salicylaldehyde Derivatives%Spectral Characterization and Antimicrobial Activity of Some Schiff Bases Derived from 4-Chloro-2-aminophenol and Various Salicylaldehyde Derivatives

    Institute of Scientific and Technical Information of China (English)

    Cinarli, Adem; Gurbuz, Demet; Tavman, Aydin; Birteksoz, A. Seher

    2012-01-01

    A series of N-(5-chloro-2-hydroxyphenyl)-(3/4/5-substituted)-salicylaldimines (I--XI) were synthesized using appropriate synthetic route. Their structures were characterized by FT-IR, UV-Visible, ESI-MS, 1H and 13C NMR spectroscopic techniques and analytical methods. The crystal structure of N-(5-chloro-2-hydroxyphenyl)-5-bromo- salicylaldimine (V) was determined by X-ray diffraction at room temperature. Relationship between the melting points and the structures of the compounds was examined. Antimicrobial activity of the compounds was evaluated against Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Proteus mirabilis. Antifungal activities were reported for Candida albicans. Schiff bases showed considerable antimicrobial activity against S. aureus, S, epidermidis and C. albicans. N-(5-Chloro-2-hydroxyphenyl)-3-hydroxy-salieylaldimine (II) has the broadest and highest antimicrobial activity according to the others.

  4. An efficient hybrid causative event-based approach for deriving the annual flood frequency distribution

    Science.gov (United States)

    Thyer, Mark; Li, Jing; Lambert, Martin; Kuczera, George; Metcalfe, Andrew

    2015-04-01

    Flood extremes are driven by highly variable and complex climatic and hydrological processes. Derived flood frequency methods are often used to predict the flood frequency distribution (FFD) because they can provide predictions in ungauged catchments and evaluate the impact of land-use or climate change. This study presents recent work on development of a new derived flood frequency method called the hybrid causative events (HCE) approach. The advantage of the HCE approach is that it combines the accuracy of the continuous simulation approach with the computational efficiency of the event-based approaches. Derived flood frequency methods, can be divided into two classes. Event-based approaches provide fast estimation, but can also lead to prediction bias due to limitations of inherent assumptions required for obtaining input information (rainfall and catchment wetness) for events that cause large floods. Continuous simulation produces more accurate predictions, however, at the cost of massive computational time. The HCE method uses a short continuous simulation to provide inputs for a rainfall-runoff model running in an event-based fashion. A proof-of-concept pilot study that the HCE produces estimates of the flood frequency distribution with similar accuracy as the continuous simulation, but with dramatically reduced computation time. Recent work incorporated seasonality into the HCE approach and evaluated with a more realistic set of eight sites from a wide range of climate zones, typical of Australia, using a virtual catchment approach. The seasonal hybrid-CE provided accurate predictions of the FFD for all sites. Comparison with the existing non-seasonal hybrid-CE showed that for some sites the non-seasonal hybrid-CE significantly over-predicted the FFD. Analysis of the underlying cause of whether a site had a high, low or no need to use seasonality found it was based on a combination of reasons, that were difficult to predict apriori. Hence it is recommended

  5. First space-based derivation of the global atmospheric methanol emission fluxes

    Science.gov (United States)

    Stavrakou, T.; Guenther, A.; Razavi, A.; Clarisse, L.; Clerbaux, C.; Coheur, P.-F.; Hurtmans, D.; Karagulian, F.; de Mazière, M.; Vigouroux, C.; Amelynck, C.; Schoon, N.; Laffineur, Q.; Heinesch, B.; Aubinet, M.; Rinsland, C.; Müller, J.-F.

    2011-05-01

    This study provides improved methanol emission estimates on the global scale, in particular for the largest methanol source, the terrestrial biosphere, and for biomass burning. To this purpose, one complete year of spaceborne measurements of tropospheric methanol columns retrieved for the first time by the thermal infrared sensor IASI aboard the MetOp satellite are compared with distributions calculated by the IMAGESv2 global chemistry-transport model. Two model simulations are performed using a priori biogenic methanol emissions either from the new MEGANv2.1 emission model, which is fully described in this work and is based on net ecosystem flux measurements, or from a previous parameterization based on net primary production by Jacob et al. (2005). A significantly better model performance in terms of both amplitude and seasonality is achieved through the use of MEGANv2.1 in most world regions, with respect to IASI data, and to surface- and air-based methanol measurements, even though important discrepancies over several regions are still present. As a second step of this study, we combine the MEGANv2.1 and the IASI column abundances over continents in an inverse modelling scheme based on the adjoint of the IMAGESv2 model to generate an improved global methanol emission source. The global optimized source totals 187 Tg yr-1 with a contribution of 100 Tg yr-1 from plants, only slightly lower than the a priori MEGANv2.1 value of 105 Tg yr-1. Large decreases with respect to the MEGANv2.1 biogenic source are inferred over Amazonia (up to 55 %) and Indonesia (up to 58 %), whereas more moderate reductions are recorded in the Eastern US (20-25 %) and Central Africa (25-35 %). On the other hand, the biogenic source is found to strongly increase in the arid and semi-arid regions of Central Asia (up to a factor of 5) and Western US (factor of 2), probably due to a source of methanol specific to these ecosystems which is unaccounted for in the MEGANv2.1 inventory. The most

  6. Analyzing Signal Attenuation in PFG Anomalous Diffusion via a Modified Gaussian Phase Distribution Approximation Based on Fractal Derivative Model

    CERN Document Server

    Lin, Guoxing

    2016-01-01

    Pulsed field gradient (PFG) has been increasingly employed to study anomalous diffusions in Nuclear Magnetic Resonance (NMR) and Magnetic Resonance Imaging (MRI). However, the analysis of PFG anomalous diffusion is complicated. In this paper, a fractal derivative model based modified Gaussian phase distribution method is proposed to describe PFG anomalous diffusion. By using the phase distribution obtained from the effective phase shift diffusion method based on fractal derivatives, and employing some of the traditional Gaussian phase distribution approximation techniques, a general signal attenuation expression for free fractional diffusion is derived. This expression describes a stretched exponential function based attenuation, which is distinct from both the exponential attenuation for normal diffusion obtained from conventional Gaussian phase distribution approximation, and the Mittag-Leffler function based attenuation for anomalous diffusion obtained from fractional derivative. The obtained signal attenu...

  7. Comparison of function approximation, heuristic, and derivative-based methods for automatic calibration of computationally expensive groundwater bioremediation models

    Science.gov (United States)

    Mugunthan, Pradeep; Shoemaker, Christine A.; Regis, Rommel G.

    2005-11-01

    The performance of function approximation (FA) methods is compared to heuristic and derivative-based nonlinear optimization methods for automatic calibration of biokinetic parameters of a groundwater bioremediation model of chlorinated ethenes on a hypothetical and a real field case. For the hypothetical case, on the basis of 10 trials on two different objective functions, the FA methods had the lowest mean and smaller deviation of the objective function among all algorithms for a combined Nash-Sutcliffe objective and among all but the derivative-based algorithm for a total squared error objective. The best algorithms in the hypothetical case were applied to calibrate eight parameters to data obtained from a site in California. In three trials the FA methods outperformed heuristic and derivative-based methods for both objective functions. This study indicates that function approximation methods could be a more efficient alternative to heuristic and derivative-based methods for automatic calibration of computationally expensive bioremediation models.

  8. Antipathogenic effects of structurally-related Schiff base derivatives: Structure–activity relationship

    Directory of Open Access Journals (Sweden)

    Soleiman Hisaindee

    2015-11-01

    Full Text Available Eighteen structurally-related Schiff base derivatives, which belong to salicylidenebenzylamine and 2-hydroxy-1-naphthylidenebenzylamine families were prepared and characterized by spectroscopic techniques. All the synthesized compounds were screened in vitro for their antibacterial and antifungal activities. Human pathogenic gram-negative (Escherichia coli, Proteus mirabilis, Pseudomonas aeruginosa and Serratia marcescens, gram-positive bacteria (Staphylococcus epidermidis, Staphylococcus aureus and Bacillus subtilis, and fungi (Alternaria alternata, Aspergillus niger, Penicillium roqueforti, and Saccharomyces cerevisiae were evaluated based on their toxicity to different concentrations of Schiff base compounds. For assessment of toxicity to pathogens, a disc diffusion assay was used to test the antimicrobial properties. The results revealed some antimicrobial activities of some of the synthesized compounds. Among the tested pathogens, the synthesized salicylidinaniline derivatives show highly potent action towards Alternaria alternata. Interestingly, a compound which contains the –Cl group is only effective against gram negative bacteria, but not gram positive bacteria. No remarkable antibacterial or antifungal activities were observed in the presence of –CH3 or –Br group. Furthermore, most of the naphthalene-containing compounds show no growth retardation towards bacterial or fungal pathogens. On the other hand, the presence of –OH or –SH group at para or ortho position, respectively, on the aniline site is correlated with an increased inhibitory drug effect on all pathogens. The importance of our findings to the drug research and developments is discussed in the context of finding a correlation between the structural properties of the new drugs and their biological activities.

  9. Deriving a probabilistic syntacto-semantic grammar for biomedicine based on domain-specific terminologies.

    Science.gov (United States)

    Fan, Jung-Wei; Friedman, Carol

    2011-10-01

    Biomedical natural language processing (BioNLP) is a useful technique that unlocks valuable information stored in textual data for practice and/or research. Syntactic parsing is a critical component of BioNLP applications that rely on correctly determining the sentence and phrase structure of free text. In addition to dealing with the vast amount of domain-specific terms, a robust biomedical parser needs to model the semantic grammar to obtain viable syntactic structures. With either a rule-based or corpus-based approach, the grammar engineering process requires substantial time and knowledge from experts, and does not always yield a semantically transferable grammar. To reduce the human effort and to promote semantic transferability, we propose an automated method for deriving a probabilistic grammar based on a training corpus consisting of concept strings and semantic classes from the Unified Medical Language System (UMLS), a comprehensive terminology resource widely used by the community. The grammar is designed to specify noun phrases only due to the nominal nature of the majority of biomedical terminological concepts. Evaluated on manually parsed clinical notes, the derived grammar achieved a recall of 0.644, precision of 0.737, and average cross-bracketing of 0.61, which demonstrated better performance than a control grammar with the semantic information removed. Error analysis revealed shortcomings that could be addressed to improve performance. The results indicated the feasibility of an approach which automatically incorporates terminology semantics in the building of an operational grammar. Although the current performance of the unsupervised solution does not adequately replace manual engineering, we believe once the performance issues are addressed, it could serve as an aide in a semi-supervised solution.

  10. Cost comparison of {sup 111}In-DTPA-octreotide scintigraphy and {sup 68}Ga-DOTATOC PET/CT for staging enteropancreatic neuroendocrine tumours

    Energy Technology Data Exchange (ETDEWEB)

    Schreiter, Nils F.; Brenner, Winfried; Buchert, Ralph; Prasad, Vikas [Charite - Universitaetsmedizin Berlin, Department of Nuclear Medicine, Berlin (Germany); Nogami, Munenobu [University Hospital, Department of Radiology and Nuclear Medicine, Nankoku, Kochi (Japan); Huppertz, Alexander [Imaging Science Institute Charite Berlin, Berlin (Germany); Pape, Ulrich-Frank [Charite - Universitaetsmedizin Berlin, Department of Gastroenterology, Berlin (Germany); Hamm, Bernd; Maurer, Martin H. [Charite - Universitaetsmedizin Berlin, Department of Radiology, Berlin (Germany)

    2012-01-15

    Although somatostatin receptor positron emission tomography (PET)/CT is gaining increasing popularity and has shown its diagnostic superiority in several studies, {sup 111}In-diethylenetriaminepentaacetic acid (DTPA)-octreotide is still the current standard for diagnosis of neuroendocrine tumours (NET). The aim of this study was to compare the costs for the two diagnostic tests and the respective consequential costs. From January 2009 to July 2009, 51 consecutive patients with enteropancreatic NET who underwent contrast-enhanced {sup 68}Ga-DOTATOC PET/CT (n = 29) or {sup 111}In-DTPA-octreotide (mean 3 whole-body scans plus 1.6 low-dose single photon emission computed tomography/CT; n = 22) were included. For cost analysis, direct costs (equipment) and variable costs (material, labour) per examination were calculated. Additionally required CT and/or MRI examinations within the staging process were assessed as consequential costs. An additional deterministic sensitivity analysis was performed. A {sup 68}Ga-DOTATOC PET/CT examination yielded total costs (equipment, personnel and material costs) of 548 EUR. On the other hand, an {sup 111}In-DTPA-octreotide examination resulted in 827 EUR total costs. Costs for equipment and material had a share of 460 EUR/720 EUR for {sup 68}Ga-DOTATOC/{sup 111}In-DTPA-octreotide and labour costs of 89 EUR/106 EUR. With {sup 68}Ga-DOTATOC additional MRI had to be performed in 7% of the patients resulting in a mean of 20 EUR for supplementary imaging per patient; 82% of patients with {sup 111}In-DTPA-octreotide needed additional MRI and/or CT resulting in mean additional costs of 161 EUR per patient. {sup 68}Ga-DOTATOC PET/CT was considerably cheaper than {sup 111}In-DTPA-octreotide with respect to both material and personnel costs. Furthermore, by using {sup 68}Ga-DOTATOC PET/CT considerably fewer additional examinations were needed reducing the consequential costs significantly. (orig.)

  11. Spatial variability of soil total and DTPA-extractable cadmium caused by long-term application of phosphate fertilizers, crop rotation, and soil characteristics.

    Science.gov (United States)

    Jafarnejadi, A R; Sayyad, Gh; Homaee, M; Davamei, A H

    2013-05-01

    Increasing cadmium (Cd) accumulation in agricultural soils is undesirable due to its hazardous influences on human health. Thus, having more information on spatial variability of Cd and factors effective to increase its content on the cultivated soils is very important. Phosphate fertilizers are main contamination source of cadmium (Cd) in cultivated soils. Also, crop rotation is a critical management practice which can alter soil Cd content. This study was conducted to evaluate the effects of long-term consumption of the phosphate fertilizers, crop rotations, and soil characteristics on spatial variability of two soil Cd species (i.e., total and diethylene triamine pentaacetic acid (DTPA) extractable) in agricultural soils. The study was conducted in wheat farms of Khuzestan Province, Iran. Long-term (27-year period (1980 to 2006)) data including the rate and the type of phosphate fertilizers application, the respective area, and the rotation type of different regions were used. Afterwards, soil Cd content (total or DTPA extractable) and its spatial variability in study area (400,000 ha) were determined by sampling from soils of 255 fields. The results showed that the consumption rate of di-ammonium phosphate fertilizer have been varied enormously in the period study. The application rate of phosphorus fertilizers was very high in some subregions with have extensive agricultural activities (more than 95 kg/ha). The average and maximum contents of total Cd in the study region were obtained as 1.47 and 2.19 mg/kg and DTPA-extractable Cd as 0.084 and 0.35 mg/kg, respectively. The spatial variability of Cd indicated that total and DTPA-extractable Cd contents were over 0.8 and 0.1 mg/kg in 95 and 25 % of samples, respectively. The spherical model enjoys the best fitting and lowest error rate to appraise the Cd content. Comparing the phosphate fertilizer consumption rate with spatial variability of the soil cadmium (both total and DTPA extractable) revealed the high

  12. Research progress in muscle-derived stem cells Literature retrieval results based on international database

    Institute of Scientific and Technical Information of China (English)

    Li Zhang; Wei Wang

    2012-01-01

    OBJECTIVE: To identify global research trends of muscle-derived stem cells (MDSCs) using a bibliometric analysis of the Web of Science, Research Portfolio Online Reporting Tools of the National Institutes of Health (NIH), and the Clinical Trials registry database (ClinicalTrials.gov). DATA RETRIEVAL: We performed a bibliometric analysis of data retrievals for MDSCs from 2002 to 2011 using the Web of Science, NIH, and ClinicalTrials.gov. MAIN OUTCOME MEASURES: (1) Type of literature; (2) annual publication output; (3) distribution according to journals; (4) distribution according to country; (5) distribution according to institution; (6) top cited authors over the last 10 years; (7) projects financially supported by the NIH; and (8) clinical trials registered.RESULTS: (1) In all, 802 studies on MDSCs appeared in the Web of Science from 2002 to 2011, almost half of which derived from American authors and institutes. The number of studies on MDSCs has gradually increased over the past 10 years. Most papers on MDSCs appeared in journals with a particular focus on cell biology research, such as Experimental Cell Research, Journal of Cell Science, and PLoS One. (2) Eight MDSC research projects have received over US$6 billion in funding from the NIH. The current project led by Dr. Johnny Huard of the University of Pittsburgh-"Muscle-Based Tissue Engineering to Improve Bone Healing"-is supported by the NIH. Dr. Huard has been the most productive and top-cited author in the field of gene therapy and adult stem cell research in the Web of Science over last 10 years. (3) On ClinicalTrials.gov, "Muscle Derived Cell Therapy for Bladder Exstrophy Epispadias Induced Incontinence" Phase 1 is registered and sponsored by Johns Hopkins University and has been led by Dr. John P. Gearhart since November 2009.CONCLUSION: From our analysis of the literature and research trends, we found that MDSCs may offer further benefits in regenerative medicine.

  13. Intercellular imaging by a polyarginine derived cell penetrating peptide labeled magnetic resonance contrast agent,diethylenetriamine pentaacetic acid gadolinium

    Institute of Scientific and Technical Information of China (English)

    GUO You-min; LIU Min; YANG Jun-le; GUO Xiao-juan; WANG Si-cen; DUAN Xiao-yi; WANG Peng

    2007-01-01

    Background The cellular plasma membrane represents a natural barrier to many exogenous molecules including magnetic resonance (MR) contrast agent. Cell penetrating peptide (CPP) is used to internalize proteins, peptides, and radionuclide. This study was undertaken to assess the value of a new intracellular MR contrast medium, CPP labeled diethylenetriamine pentaacetic acid gadolinium (Gd-DTPA) in molecular imaging in vitro. Methods Fluorescein-5-isothiocyanate (FITC) and Gd-DTPA respectively labeled with CPP (FITC-CPP, Gd-DTPA-CPP) were synthesized by the solid-phase method. Human hepatic cancer cell line-HepG2 was respectively stained by FITC-CPP and FITC to observe the uptake and intracellular distribution. HepG2 was respectively incubated with 100 nmol/ml Gd-DTPA-CPP for 0, 10, 30, 60 minutes, and imaged by MR for studying the relationship between the incubation time and T1WI signal. The cytotoxicity to NIH3T3 fibroblasts cells was measured by 3-(4,5-dimethylthiazol-2-yl)- 2,5-diphenyltetrazolium bromide reduction assay (MTT). Results The molecular weights of CPP labeled imaging agents, which were determined by MALDI mass spectrometry (FITC-CPP MW=2163.34, Gd-DTPA-CPP MW=2285.99), were similar to the calculated molecular weights. Confocal microscopy suggested HepG2 translocated FITC-CPP in cytoplasm and nucleus independent with the incubation temperature. MR images showed HepG2 uptaken Gd-DTPA-CPP had a higher T1 weighted imaging (T1WI) signal, and that the T1WI signal intensity was increasing in a time-dependent manner (r=0.972, P=0.001), while the signal intensity between the cells incubated by Gd-DTPA for 60 minutes and the controlled cells was not significantly different (P=0.225). By MTT, all concentrations from 50 nmol/ml to 200 nmol/ml had no significant (F=0.006, P=1.000) effect on cell viability of mouse NIH3T3 fibroblasts, compared with the control group. Conclusions The newly constructed CPP based on polyarginine can translocate cells by carrying FITC

  14. Robust Analysis of Network-Based Real-Time Kinematic for GNSS-Derived Heights

    Directory of Open Access Journals (Sweden)

    Tae-Suk Bae

    2015-10-01

    Full Text Available New guidelines and procedures for real-time (RT network-based solutions are required in order to support Global Navigation Satellite System (GNSS derived heights. Two kinds of experiments were carried out to analyze the performance of the network-based real-time kinematic (RTK solutions. New test marks were installed in different surrounding environments, and the existing GPS benchmarks were used for analyzing the effect of different factors, such as baseline lengths, antenna types, on the final accuracy and reliability of the height estimation. The RT solutions are categorized into three groups: single-base RTK, multiple-epoch network RTK (mRTN, and single-epoch network RTK (sRTN. The RTK solution can be biased up to 9 mm depending on the surrounding environment, but there was no notable bias for a longer reference base station (about 30 km In addition, the occupation time for the network RTK was investigated in various cases. There is no explicit bias in the solution for different durations, but smoother results were obtained for longer durations. Further investigation is needed into the effect of changing the occupation time between solutions and into the possibility of using single-epoch solutions in precise determination of heights by GNSS.

  15. Robust Analysis of Network-Based Real-Time Kinematic for GNSS-Derived Heights.

    Science.gov (United States)

    Bae, Tae-Suk; Grejner-Brzezinska, Dorota; Mader, Gerald; Dennis, Michael

    2015-10-26

    New guidelines and procedures for real-time (RT) network-based solutions are required in order to support Global Navigation Satellite System (GNSS) derived heights. Two kinds of experiments were carried out to analyze the performance of the network-based real-time kinematic (RTK) solutions. New test marks were installed in different surrounding environments, and the existing GPS benchmarks were used for analyzing the effect of different factors, such as baseline lengths, antenna types, on the final accuracy and reliability of the height estimation. The RT solutions are categorized into three groups: single-base RTK, multiple-epoch network RTK (mRTN), and single-epoch network RTK (sRTN). The RTK solution can be biased up to 9 mm depending on the surrounding environment, but there was no notable bias for a longer reference base station (about 30 km) In addition, the occupation time for the network RTK was investigated in various cases. There is no explicit bias in the solution for different durations, but smoother results were obtained for longer durations. Further investigation is needed into the effect of changing the occupation time between solutions and into the possibility of using single-epoch solutions in precise determination of heights by GNSS.

  16. Synthesis, characterization and antibacterial studies of ruthenium(III) complexes derived from chitosan schiff base.

    Science.gov (United States)

    Vadivel, T; Dhamodaran, M

    2016-09-01

    Chitosan can be modified chemically by condensation reaction of deacetylated chitosan with aldehyde in homogeneous phase. This condensation is carried by primary amine (NH2) with aldehyde (CHO) to form corresponding schiff base. The chitosan biopolymer schiff base derivatives are synthesized with substituted aldehydes namely 4-hydroxy-3-methoxy benzaldehyde, 2-hydroxy benzaldehyde, and 2-hydroxy-3-methoxy benzaldehyde, becomes a complexing agent or ligand. The Ruthenium(III) complexes were obtained by complexation of Ruthenium with schiff base ligands and this product exhibits as an excellent solubility and more biocompatibility. The novel series of schiff base Ruthenium(III) complexes are characterized by Elemental analysis, FT-IR spectroscopy, and Thermo-gravimetric analysis (TGA). The synthesized complexes have been subjected to antibacterial study. The antibacterial results indicated that the antibacterial activity of the complexes were more effective against Gram positive and Gram negative pathogenic bacteria. These findings are giving suitable support for developing new antibacterial agent and expand our scope for applications.

  17. Synthesis and characterization of nickel complexes derived from Schiff-Bases

    Directory of Open Access Journals (Sweden)

    Mirian Y. Matsumoto

    2012-06-01

    Full Text Available Aromatic Schiff-Bases constitute a large class of organic compounds containing the imine group (C=N [1-3]. 2-hydroxybenzaldehyde (salicylaldehyde which has been frequently used for the synthesis of aromatic Schiff-Bases, it has many biological applications, such as analgesics, anti-inflammatory, antibacterial, antiviral and bactericidal activities [4-6]. From above, this project proposed the synthesis of three Schiff bases derived from salicylaldehyde prepared by condensation between salicylaldehyde with different p-substituted anilines (H, NO2 and MeO. Schiff-Bases synthesized were used as starting compound for the synthesis of nickel complexes. The synthesis of nickel complexes were carried out by adding of alcoholic solution of ligands (2 eq. in aqueous solution of NiCl2.6 H2O (1 eq. contain KOH (1eq. 3 hours. After thisMatsumoto et al. time, the reaction was kept to r.t. to obtain a precipitates. The precipitates were filtered under vacuum and they were characterized by GC-MS, FT-IR and UV-Vis

  18. Analyzing signal attenuation in PFG anomalous diffusion via a modified Gaussian phase distribution approximation based on fractal derivative model

    Science.gov (United States)

    Lin, Guoxing

    2017-02-01

    Pulsed field gradient (PFG) technique is a noninvasive tool, and has been increasingly employed to study anomalous diffusions in Nuclear Magnetic Resonance (NMR) and Magnetic Resonance Imaging (MRI). However, the analysis of PFG anomalous diffusion is much more complicated than normal diffusion. In this paper, a fractal derivative model based modified Gaussian phase distribution method is proposed to describe PFG anomalous diffusion. By using the phase distribution obtained from the effective phase shift diffusion method based on fractal derivatives, and employing some of the traditional Gaussian phase distribution approximation techniques, a general signal attenuation expression for free fractional diffusion is derived. This expression describes a stretched exponential function based attenuation, which is distinct from both the exponential attenuation for normal diffusion obtained from conventional Gaussian phase distribution approximation, and the Mittag-Leffler function based attenuation for anomalous diffusion obtained from fractional derivative. The obtained signal attenuation expression can analyze the finite gradient pulse width (FGPW) effect. Additionally, it can generally be applied to all three types of PFG fractional diffusions classified based on time derivative order α and space derivative order β. These three types of fractional diffusions include time-fractional diffusion with { 0 reported results based on effective phase shift diffusion equation method and instantaneous signal attenuation method. This method provides a new, convenient approximation formalism for analyzing PFG anomalous diffusion experiments. The expression that can simultaneously interpret general fractional diffusion and FGPW effect could be especially important in PFG MRI, where the narrow gradient pulse limit cannot be satisfied.

  19. New hybrid magnetic nanoparticles based on chitosan-maltose derivative for antitumor drug delivery.

    Science.gov (United States)

    Alupei, Liana; Peptu, Catalina Anisoara; Lungan, Andreea-Maria; Desbrieres, Jacques; Chiscan, Ovidiu; Radji, Sadia; Popa, Marcel

    2016-11-01

    The aim of the present study is to obtain, for the first time, polymer magnetic nanoparticles based on the chitosan-maltose derivative and magnetite. By chemically modifying the chitosan, its solubility in aqueous media was improved, which in turn facilitates the nanoparticles' preparation. Resulting polymers exhibit enhanced hydrophilia, which is an important factor in increasing the retention time of nanoparticles in the blood flow. The preparation of nanoparticles relied on the double crosslinking technique (ionic and covalent) in reverse emulsion which ensures the mechanical stability of the polymer carrier. The characterization of both the chitosan derivative and nanoparticles was accomplished by Fourier Transform Infrared Spectroscopy, Nuclear Magnetic Resonance Spectroscopy, Scanning Electron Microscopy, Transmission Electron Microscopy, Atomic Force Microscopy, Vibrating Sample Magnetometry, and Thermogravimetric Analysis. The evaluation of morphological, dimensional, structural, and magnetical properties, as well as thermal stability and swelling behavior of nanoparticles was made from the point of view of the polymer/magnetite ratio. The study of 5-Fluorouracil loading and release kinetics as well as evaluating the cytotoxicity and hemocompatibility of nanoparticles justify their adequate behavior in their potential use as devices for targeted transport of antitumor drugs.

  20. Design of PID controller with incomplete derivation based on ant system algorithm

    Institute of Scientific and Technical Information of China (English)

    Guanzheng TAN; Qingdong ZENG; Wenbin LI

    2004-01-01

    A new and intelligent design method for PID controller with incomplete derivation is proposed based on the ant system algorithm (ASA).For a given control system with this kind of PID controller,a group of optimal PID controller parameters K*p,T*i, and T*d can be obtained by taking the overshoot,settling time,and steady-state error of the system's unit step response as the performance indexes and by use of our improved ant system algorithm.K*p,T*i, and T*d can be used in real-time control.This kind of controller is called the ASA-PID controller with incomplete derivation.To verify the performance of the ASA-PID controller,three different typical transfer functions were tested,and three existing typical tuning methods of PID controller parameters,including the Ziegler-Nichols method (ZN),the genetic algorithm (GA),and the simulated annealing (SA),were adopted for comparison.The simulation results showed that the ASA-PID controller can be used to control different objects and has better performance compared with the ZN-PID and GA-PID controllers,and comparable performance compared with the SA-PID controller.

  1. Polyurethane and polyurea nanoparticles based on polyoxyethylene castor oil derivative surfactant suitable for endovascular applications.

    Science.gov (United States)

    Morral-Ruíz, Genoveva; Melgar-Lesmes, Pedro; García, María Luísa; Solans, Conxita; García-Celma, María José

    2014-01-30

    The design of new, safe and effective nanotherapeutic systems is an important challenge for the researchers in the nanotechnology area. This study describes the formation of biocompatible polyurethane and polyurea nanoparticles based on polyoxyethylene castor oil derivative surfactant formed from O/W nano-emulsions by polymerization at the droplet interfaces in systems composed by aqueous solution/Kolliphor(®) ELP/medium chain triglyceride suitable for intravenous administration. Initial nano-emulsions incorporating highly hydrophilic materials were prepared by the phase inversion composition (PIC) method. After polymerization, nanoparticles with a small particle diameter (25-55 nm) and low polydispersity index were obtained. Parameters such as concentration of monomer, O/S weight ratio as well as the polymerization temperature were crucial to achieve a correct formation of these nanoparticles. Moreover, FT-IR studies showed the full conversion of the monomer to polyurethane and polyurea polymers. Likewise the involvement of the surfactant in the polymerization process through their nucleophilic groups to form the polymeric matrix was demonstrated. This could mean a first step in the development of biocompatible systems formulated with polyoxyethylene castor oil derivative surfactants. In addition, haemolysis and cell viability assays evidenced the good biocompatibility of KELP polyurethane and polyurea nanoparticles thus indicating the potential of these nanosystems as promising drug carriers.

  2. White light generation by carbonyl based indole derivatives due to proton transfer: an efficient fluorescence sensor.

    Science.gov (United States)

    Singla, Nidhi; Bhadram, Venkata Srinu; Narayana, Chandrabhas; Chowdhury, Papia

    2013-04-01

    The motivation of the present work is to understand the optical, chemical, and electrical aspects of the proton transfer mechanism of indole (I) and some carbonyl based indole derivatives: indole-3-carboxaldehyde (I3C) and indole-7-carboxaldehyde (I7C) for both powder form and their liquid solution. Structural information for indole derivatives (isolated molecule and in solution) is obtained with density functional theory (DFT) and time dependent DFT (TD-DFT) methods. Calculated transition energies are used to generate UV-vis, FTIR, Raman, and NMR spectra which are later verified with the experimental spectra. The occurrence of different conformers [cis (N(c)), trans (N(t)), and zwitterion (Z*)] have been interpreted by Mulliken charge, natural bond orbital (NBO) analysis, and polarization versus electric field (P-E loop) studies. (1)H and (13)C NMR and molecular vibrational frequencies of the fundamental modes established the stability of Nc due to the presence of intramolecular hydrogen bonding (IHB) in the ground state (S0). Computed/experimental UV-vis absorption/emission studies reveal the creation of new species: zwitterion (Z*) and anion (A*) in the excited state (S1) due to excited state intramolecular and intermolecular proton transfer (ESI(ra)PT and ESI(er)PT). Increased electrical conductivity (σ(ac)) with temperature and increased ferroelectric polarization at higher field verifies proton conduction in I7C.

  3. FORTRAN program for analyzing ground-based radar data: Usage and derivations, version 6.2

    Science.gov (United States)

    Haering, Edward A., Jr.; Whitmore, Stephen A.

    1995-01-01

    A postflight FORTRAN program called 'radar' reads and analyzes ground-based radar data. The output includes position, velocity, and acceleration parameters. Air data parameters are also provided if atmospheric characteristics are input. This program can read data from any radar in three formats. Geocentric Cartesian position can also be used as input, which may be from an inertial navigation or Global Positioning System. Options include spike removal, data filtering, and atmospheric refraction corrections. Atmospheric refraction can be corrected using the quick White Sands method or the gradient refraction method, which allows accurate analysis of very low elevation angle and long-range data. Refraction properties are extrapolated from surface conditions, or a measured profile may be input. Velocity is determined by differentiating position. Accelerations are determined by differentiating velocity. This paper describes the algorithms used, gives the operational details, and discusses the limitations and errors of the program. Appendices A through E contain the derivations for these algorithms. These derivations include an improvement in speed to the exact solution for geodetic altitude, an improved algorithm over earlier versions for determining scale height, a truncation algorithm for speeding up the gradient refraction method, and a refinement of the coefficients used in the White Sands method for Edwards AFB, California. Appendix G contains the nomenclature.

  4. Properties of Thermosets Derived from Chemically Modified Triglycerides and Bio-Based Comonomers

    Directory of Open Access Journals (Sweden)

    Evan S. Beach

    2013-12-01

    Full Text Available A series of materials was prepared by curing acrylated epoxidized soybean oil (AESO and dibutyl itaconate (DBI or ethyl cinnamate (EC comonomers to provide examples of thermosets with a high proportion of bio-based carbon, in accordance with the principles of green chemistry. The comonomers, representative of cellulose-derived (DBI or potentially lignin-derived (EC raw materials, were tested at levels of 25%, 33%, and 50% by mass and the resulting products were characterized by infrared spectroscopy, thermogravimetric analysis, and dynamic mechanical analysis. Both DBI and EC were incorporated into the thermosets to a high extent (>90% at all concentrations tested. The AESO-DBI and AESO-EC blends showed substantial degradation at 390–400 °C, similar to pure AESO. Glass transition temperatures decreased as comonomer content increased; the highest Tg of 41.4 °C was observed for AESO-EC (3:1 and the lowest Tg of 1.4 °C was observed for AESO-DBI (1:1. Accordingly, at 30 °C the storage modulus values were highest for AESO-EC (3:1, 37.0 MPa and lowest for AESO-DBI (1:1, 1.5 MPa.

  5. Image Clustering Method Based on Density Maps Derived from Self-Organizing Mapping: SOM

    Directory of Open Access Journals (Sweden)

    Kohei Arai

    2012-07-01

    Full Text Available A new method for image clustering with density maps derived from Self-Organizing Maps (SOM is proposed together with a clarification of learning processes during a construction of clusters. It is found that the proposed SOM based image clustering method shows much better clustered result for both simulation and real satellite imagery data. It is also found that the separability among clusters of the proposed method is 16% longer than the existing k-mean clustering. It is also found that the separability among clusters of the proposed method is 16% longer than the existing k-mean clustering. In accordance with the experimental results with Landsat-5 TM image, it takes more than 20000 of iteration for convergence of the SOM learning processes.

  6. SYNTHESIS AND ANTIBACTERIAL ACTIVITY OF SCHIFF BASE DERIVATIVES OF 4-CHLORO-3-NITROBENZOIC ACID

    Directory of Open Access Journals (Sweden)

    Kumari Santosh

    2012-12-01

    Full Text Available 4-chloro-3-nitrobenzoic acid was selected as nucleus structure having good antibacterial property. It was thought worthwhile to synthesize Schiff base derivatives of 4-chloro-3-nitrobenzoic acid in search of better antibacterial agents. The compounds were synthesized by first reducing nitro group in presence of Sn/HCl to 3-amino-4-chlorobenzoic acid, which on treatment with substituted benzaldehyde afforded the synthesis of the envisaged compounds. The synthesized compounds were purified and chemically characterized. They were screened for their antibacterial activity and result suggested that SKY-1, SKY-5 and SKY-8 were highly active against both Gram (+ and Gram(- bacteria like B.subtilis, S.aureus and E.coli whereas all are were found moderately to inactive against P.aeruginosa.

  7. AN EXPLORATION ON THE SYNTHESIS AND BIO-APPLICATIONS OF DERIVATIVES OF HETEROCYCLIC MANNICH BASES

    Directory of Open Access Journals (Sweden)

    P. S. Manjula

    2016-01-01

    Full Text Available The Mannich reaction is a three-component reaction of an aldehyde, a primary or secondary amine and a ketone which is one of the most powerful C-C bond forming reactions in organic synthesis. It leads to β-amino carbonyl compounds, which are useful for the syntheses of nitrogen containing compounds, such as natural products and medicinally relevant compounds. The versatility and potential of these compounds to introduce both functional and structural diversity using the Mannich reaction have stimulated the creativity of chemists. Keeping in view of the importance of this organic moiety in the field of medicine and biology here an attempt has been made to review the synthesis and biological importance of heterocyclic Mannich base derivatives.

  8. Design of PID controller with incomplete derivation based on differential evolution algorithm

    Institute of Scientific and Technical Information of China (English)

    Wu Lianghong; Wang Yaonan; Zhou Shaowu; Tan Wen

    2008-01-01

    To determine the optimal or near optimal parameters of PID controller with incomplete derivation, a novel design method based on differential evolution (DE) algorithm is presented. The controller is called DE-PID controller. To overcome the disadvantages of the integral performance criteria in the frequency domain such as IAE, ISE, and ITSE, a new performance criterion in the time domain is proposed. The optimization procedures employing the DE algorithm to search the optimal or near optimal PID controller parameters of a control system are demonstrated in detail. Three typical control systems are chosen to test and evaluate the adaptation and robustness of the proposed DE-PID controller. The simulation results show that the proposed approach has superior features of easy implementation, stable convergence characteristic, and good computational efficiency. Compared with the ZN, GA, and ASA, the proposed design method is indeed more efficient and robust in improving the step response of a control system.

  9. Aliphatic amine responsive organogel system based on a simple naphthalimide derivative.

    Science.gov (United States)

    Cao, Xinhua; Zhang, Tingting; Gao, Aiping; Li, Keli; Cheng, Qiuli; Song, Lijuan; Zhang, Min

    2014-09-07

    A new gelator 1 based on a simple naphthalimide derivative was synthesized and fully characterized. It was found that the organogel 1 was formed only in a mixed solvent of methanol and H2O (1/1, v/v). The organogel was thoroughly characterized by using various microscopic techniques including field-emission scanning electron microscopy (FESEM), X-ray diffraction (XRD), and UV-vis, fluorescence and Fourier transform infrared (FTIR) spectroscopy. Hydrogen bonds were the main driving force for the organogel formation. Interestingly, the organogel 1 exhibited the ability to distinguish aliphatic amines from aromatic amines. The gel state and fluorescence emission intensity were both changed after two minutes after the addition of aliphatic amines. This organogel system could be applied in the detection of aliphatic amine pollutants.

  10. Catecholase activity investigations using in situ copper complexes continuing Schiff base derivatives with a theoretical calculation

    Directory of Open Access Journals (Sweden)

    A. Djedouani

    2015-03-01

    Full Text Available The study of catecholase activity of a series of Schiff base compounds using in situ copper complexes of 4-hydroxy-6-methyl-3-(1-(phenyliminoethyl-2H-pyran-2-one derivatives has been reported. The reaction rate depends on four parameters: The nature of the substitution in para position to the benzene ring, the nature of counter anion, the concentration of ligand and the nature of solvent. The highest rate activity is given by complex resulting from one equivalent of ligand L2 and two equivalents of copper acetate in methanol, which equal to 62.25 µmol.min-1.L-1.In other part, a theoretical study of such ligands using the semi-empirical method AM1 were also investigated. A good relationship founded between the maximal reaction rate (Vmax and the HOMO energy (Pearson correlation: r=-0.794.

  11. Synthesis and Biological Evaluation of Mannich Bases of Isoxazoline Derivatives as Novel Anti-Microbial Agents

    Directory of Open Access Journals (Sweden)

    Pawar Sudhir

    2012-01-01

    Full Text Available A novel series of compounds were synthesized by condensation reaction of substituted acetophenone (1a-b with substituted aldehyde (2a-b in presence of alcoholic sodium hydroxide to get intermediate chalcones (3a-c, which were further treated with hydroxylamine hydrochloride in presence of sodium hydroxide to get isoxazolines derivatives (4a-c. The latter were refluxed with substituted primary amines and formaldehyde for 6-10 h to afford Mannich bases. The synthesized compounds were characterized on the basis of their spectral (IR, 1HNMR data and evaluated for the antimicrobial activity by using Zone of Inhibition by cup plate method and Minimum Inhibitory Concentration by broth dilution method.

  12. Poly-Acrylic Acid Derivatives as Diesel Flow Improver for Paraffin-Based Daqing Diesel

    Institute of Scientific and Technical Information of China (English)

    Cuiyu Jiang; Ming Xu; Xiaoli Xi; Panlun Qi; Hongyan Shang

    2006-01-01

    Since the diesel products from paraffin-based Daqing crude oil showed low sensitivity to certain commercial diesel pour point depressant (PPDs) that resulted from the high content of paraffin, certain poly-acrylic acid derivatives (PADE) with-COOR,-COOH,-CONHR, and -COO-NH3+R groups by molecular design on the mechanics of diesel; PPDs were synthesized and evaluated as cold flow improver for Daqing 0# diesel in this paper. The pure PADE was superior to the commercial PPDs and displayed a substantial ability of wax crystals dispersion. There was a synergistic effect among the PADE and T1804 and secondary amine. The synergism clearly improved the low temperature performance of Daqing diesel products and could reduce the cold filter plugging point of 0# diesel by 6-7 ℃.

  13. The first genetic linkage map of Primulina eburnea (Gesneriaceae) based on EST-derived SNP marker

    Indian Academy of Sciences (India)

    CHEN FENG; CHAO FENG; MING KANG

    2016-06-01

    Primulina eburneais a promising candidate for domestication and floriculture, since it is easy to culture and has beautiful flow-ers. An F2population of 189 individuals was established for the construction of first-generation linkage maps based onexpressed sequence tags-derived single-nucleotide polymorphism markers using the massARRAY genotyping platform. Ofthe 232 screened markers, 215 were assigned to 18 LG according to the haploid number of chromosomes in the species. Thelinkage map spanned a total of 3774.7 cM with an average distance of 17.6 cM between adjacent markers. This linkage mapprovides a framework for identification of important genes in breeding programm

  14. STRUCTURE BASED DRUG DESIGN STUDIES ON HETEROARYL PROPANOIC ACID DERIVATIVES AS PPARΓ AGONISTS

    Directory of Open Access Journals (Sweden)

    ARUN KUMAR,MANGA RATNAM,ARCHANA KUMARI, AJAY BABU

    2013-09-01

    Full Text Available Peroxisome proliferator-activated receptors (PPARs are a group of nuclear receptorproteins. Docking studies are based on several factors. Among 15 entries of PPARγ, 2Q6S wastaken for docking analysis, as it showed 418 most favored regions, 35 in additionally allowedregion and none of the residue in disallowed regions. To carry out drug designing, moleculeswere considered from the literature in which substitution of R1 position with dihydrofurylreported to have high dock score (-14.98 Kcal/mol than the remaining analogues, with bettergeometry and interactions. Hence docking analysis using heteroaryl propionic acid derivatives asanti-diabetic agents suggest the reproducibility of active molecules being predicted bycomputational docking studies using Auto dock software.

  15. Smart photovoltaics based on dye-sensitized solar cells using photochromic spiropyran derivatives as photosensitizers

    Energy Technology Data Exchange (ETDEWEB)

    Ma, Shengbo; Ting, Hungkit; Ma, Yingzhuang; Zheng, Lingling; Zhang, Miwei [State Key Laboratory for Artificial Microstructures and Mesoscopic Physics, Department of Physics, Peking University, Beijing 100871 (China); Xiao, Lixin, E-mail: zjchen@pku.edu.cn, E-mail: lxxiao@pku.edu.cn; Chen, Zhijian, E-mail: zjchen@pku.edu.cn, E-mail: lxxiao@pku.edu.cn [State Key Laboratory for Artificial Microstructures and Mesoscopic Physics, Department of Physics, Peking University, Beijing 100871 (China); Haixi Collaborative Innovation Center for New Display Devices and Systems Integration, Fuzhou University, Fuzhou 350002 (China)

    2015-05-15

    In this paper, smart photovoltaic (SPV) devices, integrating both functions of solar cells and smart windows, was fabricated based on dye-sensitized solar cells using photochromic spiropyran derivatives SIBT as photosensitizers. SPV devices have self-regulated power conversion efficiency (PCE) and light transmission responding to the incident spectra due to the photoisomerization of SIBT. SIBT isomerize from closed-ring form to open-ring form under UV illumination, accompanied with enhanced visible light absorption and electron delocalization. Therefore, increased PCE and absorption in SPV devices were observed under UV treatment and the devices can be restored gradually to the initial status when kept in dark. The SPV devices have self-regulation of PCE and sunlight transmission responding to the changing sun spectra in different times of a day, providing a proper energy usage and a better sun-shading.

  16. A simple derivation and classification of common probability distributions based on information symmetry and measurement scale

    CERN Document Server

    Frank, Steven A

    2010-01-01

    Commonly observed patterns typically follow a few distinct families of probability distributions. Over one hundred years ago, Karl Pearson provided a systematic derivation and classification of the common continuous distributions. His approach was phenomenological: a differential equation that generated common distributions without any underlying conceptual basis for why common distributions have particular forms and what explains the familial relations. Pearson's system and its descendants remain the most popular systematic classification of probability distributions. Here, we unify the disparate forms of common distributions into a single system based on two meaningful and justifiable propositions. First, distributions follow maximum entropy subject to constraints, where maximum entropy is equivalent to minimum information. Second, different problems associate magnitude to information in different ways, an association we describe in terms of the relation between information invariance and measurement scale....

  17. Earth's gravity field modelling based on satellite accelerations derived from onboard GPS phase measurements

    Science.gov (United States)

    Guo, X.; Ditmar, P.; Zhao, Q.; Klees, R.; Farahani, H. H.

    2017-02-01

    GPS data collected by satellite gravity missions can be used for extracting the long-wavelength part of the Earth's gravity field. We propose a new data processing method which makes use of the `average acceleration' approach to gravity field modelling. In this method, satellite accelerations are directly derived from GPS carrier phase measurements with an epoch-differenced scheme. As a result, no ambiguity solutions are needed and the systematic errors that do not change much from epoch to epoch are largely eliminated. The GPS data collected by the Gravity Field and Steady-State Ocean Circulation Explorer (GOCE) satellite mission are used to demonstrate the added value of the proposed method. An analysis of the residual accelerations shows that accelerations derived in this way are more precise, with noise being reduced by about 20 and 5% at the cross-track component and the other two components, respectively, as compared to those based on kinematic orbits. The accelerations obtained in this way allow the recovery of the gravity field to a slightly higher maximum degree compared to the solution based on kinematic orbits. Furthermore, the gravity field solution has an overall better performance. Errors in spherical harmonic coefficients are smaller, especially at low degrees. The cumulative geoid height error is reduced by about 15 and 5% up to degree 50 and 150, respectively. An analysis in the spatial domain shows that large errors along the geomagnetic equator, which are caused by a high electron density coupled with large short-term variations, are substantially reduced. Finally, the new method allows for a better observation of mass transport signals. In particular, sufficiently realistic signatures of regional mass anomalies in North America and south-west Africa are obtained.

  18. Microcalorimetric Study on the Inhibition of Escherichia coil by Some Novel Pyridine Amide Schiff Base Derivatives

    Institute of Scientific and Technical Information of China (English)

    LI Chao-Hong; ZHANG Li-Xia; CAI Li-Hua; LIU Yi; HU Pei-Zhi

    2008-01-01

    The antibacterial effect of a series of novel pyridine amide Schiff base compounds on Escherichia coli was investigated by a microcalorimetric method at 37℃.The metabolic power-time curves of the bacteria treated by the compounds were obtained,and the thermokinetic parameters were analyzed,from which the antibacterial activities of these compounds were evaluated.The results show that two compounds F and G have good activity on aerobic multiplying metabolism of E.coli,with the value of IC50 106 and 113 mg/L respectively,but have no effective action on the fermentation metabolism of E.coli.The action of the compounds on the non-multiplying metabolism was investigated by taking the heat output of E.coli in the stationary phase as the guideline of the activity.The experimental results revealed that the hydrophilicity of these Schiff bases had a great influence on their antibacterial activity,which results from the bacterial cell wall structure.The antibacterial structure-activity relationship of these Schiff base derivatives was also briefly discussed.The antibacterial activity of the compounds against E.coli was as follows:compound F>G>C>D>E>B>A.

  19. Base preferences in non-templated nucleotide incorporation by MMLV-derived reverse transcriptases.

    Directory of Open Access Journals (Sweden)

    Pawel Zajac

    Full Text Available Reverse transcriptases derived from Moloney Murine Leukemia Virus (MMLV have an intrinsic terminal transferase activity, which causes the addition of a few non-templated nucleotides at the 3' end of cDNA, with a preference for cytosine. This mechanism can be exploited to make the reverse transcriptase switch template from the RNA molecule to a secondary oligonucleotide during first-strand cDNA synthesis, and thereby to introduce arbitrary barcode or adaptor sequences in the cDNA. Because the mechanism is relatively efficient and occurs in a single reaction, it has recently found use in several protocols for single-cell RNA sequencing. However, the base preference of the terminal transferase activity is not known in detail, which may lead to inefficiencies in template switching when starting from tiny amounts of mRNA. Here, we used fully degenerate oligos to determine the exact base preference at the template switching site up to a distance of ten nucleotides. We found a strong preference for guanosine at the first non-templated nucleotide, with a greatly reduced bias at progressively more distant positions. Based on this result, and a number of careful optimizations, we report conditions for efficient template switching for cDNA amplification from single cells.

  20. Base Preferences in Non-Templated Nucleotide Incorporation by MMLV-Derived Reverse Transcriptases

    Science.gov (United States)

    Zajac, Pawel; Islam, Saiful; Hochgerner, Hannah; Lönnerberg, Peter; Linnarsson, Sten

    2013-01-01

    Reverse transcriptases derived from Moloney Murine Leukemia Virus (MMLV) have an intrinsic terminal transferase activity, which causes the addition of a few non-templated nucleotides at the 3´ end of cDNA, with a preference for cytosine. This mechanism can be exploited to make the reverse transcriptase switch template from the RNA molecule to a secondary oligonucleotide during first-strand cDNA synthesis, and thereby to introduce arbitrary barcode or adaptor sequences in the cDNA. Because the mechanism is relatively efficient and occurs in a single reaction, it has recently found use in several protocols for single-cell RNA sequencing. However, the base preference of the terminal transferase activity is not known in detail, which may lead to inefficiencies in template switching when starting from tiny amounts of mRNA. Here, we used fully degenerate oligos to determine the exact base preference at the template switching site up to a distance of ten nucleotides. We found a strong preference for guanosine at the first non-templated nucleotide, with a greatly reduced bias at progressively more distant positions. Based on this result, and a number of careful optimizations, we report conditions for efficient template switching for cDNA amplification from single cells. PMID:24392002

  1. Deriving statistical significance maps for SVM based image classification and group comparisons.

    Science.gov (United States)

    Gaonkar, Bilwaj; Davatzikos, Christos

    2012-01-01

    Population based pattern analysis and classification for quantifying structural and functional differences between diverse groups has been shown to be a powerful tool for the study of a number of diseases, and is quite commonly used especially in neuroimaging. The alternative to these pattern analysis methods, namely mass univariate methods such as voxel based analysis and all related methods, cannot detect multivariate patterns associated with group differences, and are not particularly suitable for developing individual-based diagnostic and prognostic biomarkers. A commonly used pattern analysis tool is the support vector machine (SVM). Unlike univariate statistical frameworks for morphometry, analytical tools for statistical inference are unavailable for the SVM. In this paper, we show that null distributions ordinarily obtained by permutation tests using SVMs can be analytically approximated from the data. The analytical computation takes a small fraction of the time it takes to do an actual permutation test, thereby rendering it possible to quickly create statistical significance maps derived from SVMs. Such maps are critical for understanding imaging patterns of group differences and interpreting which anatomical regions are important in determining the classifier's decision.

  2. Deriving pathway maps from automated text analysis using a grammar-based approach.

    Science.gov (United States)

    Olsson, Björn; Gawronska, Barbara; Erlendsson, Björn

    2006-04-01

    We demonstrate how automated text analysis can be used to support the large-scale analysis of metabolic and regulatory pathways by deriving pathway maps from textual descriptions found in the scientific literature. The main assumption is that correct syntactic analysis combined with domain-specific heuristics provides a good basis for relation extraction. Our method uses an algorithm that searches through the syntactic trees produced by a parser based on a Referent Grammar formalism, identifies relations mentioned in the sentence, and classifies them with respect to their semantic class and epistemic status (facts, counterfactuals, hypotheses). The semantic categories used in the classification are based on the relation set used in KEGG (Kyoto Encyclopedia of Genes and Genomes), so that pathway maps using KEGG notation can be automatically generated. We present the current version of the relation extraction algorithm and an evaluation based on a corpus of abstracts obtained from PubMed. The results indicate that the method is able to combine a reasonable coverage with high accuracy. We found that 61% of all sentences were parsed, and 97% of the parse trees were judged to be correct. The extraction algorithm was tested on a sample of 300 parse trees and was found to produce correct extractions in 90.5% of the cases.

  3. GPS Interference Mitigation Using Derivative-free Kalman Filter-based RNN

    Directory of Open Access Journals (Sweden)

    W. L. Mao

    2016-09-01

    Full Text Available The global positioning system (GPS with accurate positioning and timing properties has become integral part of all applications around the world. Radio frequency interference can significantly decrease the performance of GPS receivers or even completely prohibit the acquisition or tracking of satellites. The approaches of system performances that can be further enhanced by preprocessing to reject the jamming signal will be investigated. A recurrent neural network (RNN predictor for the GPS anti-jamming applications will be proposed. The adaptive RNN predictor is utilized to accurately predict the narrowband waveform based on an unscented Kalman filter (UKF-based algorithm. The UKF algorithm as a derivative-free alternative to the extended Kalman filter (EKF in the framework of state-estimation is adopted to achieve better performance in terms of convergence rate and quality of solution. The adaptive RNN filter can be successfully applied for the suppression of interference with a number of different narrowband formats, i.e. continuous wave interference (CWI, multi-tone CWI, swept CWI and pulsed CWI, to emulate realistic circumstances. Simulation results show that the proposed UKF-based scheme can offer the superior performances to suppress the interference over the conventional methods by computing mean squared prediction error (MSPE and signal-to-noise ratio (SNR improvements.

  4. Gallic acid-based indanone derivative interacts synergistically with tetracycline by inhibiting efflux pump in multidrug resistant E. coli.

    Science.gov (United States)

    Dwivedi, Gaurav Raj; Tiwari, Nimisha; Singh, Aastha; Kumar, Akhil; Roy, Sudeep; Negi, Arvind Singh; Pal, Anirban; Chanda, Debabrata; Sharma, Ashok; Darokar, Mahendra P

    2016-03-01

    The purpose of the present study was to study the synergy potential of gallic acid-based derivatives in combination with conventional antibiotics using multidrug resistant cultures of Escherichia coli. Gallic acid-based derivatives significantly reduced the MIC of tetracycline against multidrug resistant clinical isolate of E. coli. The best representative, 3-(3',4,'5'-trimethoxyphenyl)-4,5,6-trimethoxyindanone-1, an indanone derivative of gallic acid, was observed to inhibit ethidium bromide efflux and ATPase which was also supported by in silico docking. This derivative extended the post-antibiotic effect and decreased the mutation prevention concentration of tetracycline. This derivative in combination with TET was able to reduce the concentration of TNFα up to 18-fold in Swiss albino mice. This derivative was nontoxic and well tolerated up to 300 mg/kg dose in subacute oral toxicity study in mice. This is the first report of gallic acid-based indanone derivative as drug resistance reversal agent acting through ATP-dependent efflux pump inhibition.

  5. Certified reference material for the quality control of EDTA- and DTPA-extractable trace metal contents in calcareous soil (CRM 600)

    Energy Technology Data Exchange (ETDEWEB)

    Quevauviller, P. [European Commission, Standards, Measurements and Testing Programme, Brussels (Belgium); Lachica, M.; Barahona, E. [Estacion Experimental del Zaidin, Granada (Spain); Gomez, A. [Institut Nacional de Recherche Agronomique, Station d`Agronomie, Villenave d`Ornon (France); Rauret, G. [Universidad de Barcelona, Dept. de Quimica Analitica, Barcelona (Spain); Ure, A. [University of Strathclyde, Dept. of Pure and Applied Chemistry, Glasgow (United Kingdom); Muntau, H. [European Commission, Joint Research Centre, Environment Institute, Ispra (Italy)

    1998-03-01

    Single extraction tests are commonly used to study the eco-toxicity and mobility of metals in soils, e.g. to assess the bioavailable metal fraction (and thus to estimate the related phyto-toxic and nutritional deficiency effects) and the environmentally accessible trace metals upon disposal of e.g. sediment on to a soil (e.g. contamination of ground waters). However, the lack of uniformity in the different procedures does not allow the results to be compared worldwide nor the procedures to be validated. This paper describes the interlaboratory testing of EDTA- and DTPA-extraction procedures for soil analysis, followed by the preparation of a calcareous soil reference material (CRM 600), the homogeneity and stability studies and the analytical work performed for the certification of the EDTA- and DTPA- extractable contents of some trace metals (following the standardized extraction procedures). (orig.) With 2 figs., 7 tabs., 14 refs.

  6. [Quantification of separate renal function using Tc 99m DTPA and Tc 99m DMSA. Correlations between individual isotopic data and creatinine clearance].

    Science.gov (United States)

    Chevet, D; Moisan, A; Le Pogamp, P; Le Cloirec, J; Wehbe, B; Herry, J Y

    1984-01-01

    The purpose of this prospective study (30 controls and 100 renal patients) is to compare one to each other the values of renal function measured with: 1--the DTPA 99mTc , 2--the DMSA 99mTc , and 3--the creatinine clearance. After the control values being established, correlations are done for the whole group (n = 130) between the creatinine clearance, the DTPA 99mTc clearance and the fixation rate of the DMSA 99mTc . The correlations obtained are:--Clcr Versus Cldtpa = 0,91.--Clcr Versus % Dmsa = 0,90. - Cldtpa Versus % Dmsa = 0,93. and indicate the two isotopic tests performed give similar quantitative results than creatinine clearance. Camera and computer allow to measure the separate renal function one by one, the two isotopic test giving similar values for each kidney. The results of these studies give a good concordance with the observed diseases, except for partial renal artery stenosis. The pyelic retention of DMSA 99mTc product, in condition of ureteral compression, gives a potential overestimation of this test and is a matter to debate. The extreme simplicity and facility of DMSA 99mTc procedures make this investigation accessible to any patient and give excellent morphological and functional tests. DTPA 99mTc clearances--global and separate kidney--give a more sensitive approach than DMSA 99mTc does because vascular captation , elimination rates could be focused. DTPA 99mTc procedure is to be used in patients with transplanted kidney, silent IVP kidney, contrast media contraindication or for exploring glomerular clearance without urine collection.

  7. MRI enhancement of the facial nerve with Gd-DTPA, 1; Experimental study on the enhancement mechanism used in viewing vascular permeability of the facial nerve

    Energy Technology Data Exchange (ETDEWEB)

    Yanagida, Masahiro (Kansai Medical School, Moriguchi, Osaka (Japan))

    1993-08-01

    Although there have recently been numerous reports of enhanced MRI in patients with facial palsy, the mechanism of enhancement remains largely unknown. In the present study, animal models with experimentally induced facial paralysis were prepared, and the vascular permeabilities of normal and damaged facial nerves were assessed using Evans blue albumin (EBA) as a tracer. The Gd-DTPA contents in normal and compressively damaged facial nerves were also investigated. In the normal intratemporal facial nerve, EBA remained in the vessels, and did not leak into the endoneurium. In contrast, vascular permeability was very high in the epineurium and the geniculate ganglion which showed leakage of large amounts of EBA from vessels. At the site of compression in the damaged nerve, EBA leakage was also seen in the endoneurism, indicating accentuated vascular permeability. This accentuation of vascular permeability shifted toward the distal side. However, no EBA leakage was seen on the side proximal to the site of compression. Significantly higher Gd-DTPA contents were obtained in the facial nerve on the paralytic side than in that on the normal side (p<0.001). As for differences between the distal and proximal sides, the distal side had a significantly higher Gd-DTPA content (p<0.01). Assessment of vascular permeability with EBA revealed accentuated vascular permeability on the side distal to the site of compression. These results showed the presence of a blood nerve barrier (BNB) in the facial nerve. Furthermore, the present findings suggest that the enhancement of the facial nerve on the affected side is caused by BNB destruction due to nerve damage and subsequent Gd-DTPA leakage from the vessels. Furthermore, it is suggested that the facial nerve enhancement appears to occur mainly on the distal side of the damaged portion of the nerve. (author).

  8. The Necrosis-Avid Small Molecule HQ4-DTPA as a Multimodal Imaging Agent for Monitoring Radiation Therapy-Induced Tumor Cell Death

    OpenAIRE

    Marieke A. Stammes; Maeda, Azusa; Bu, Jiachuan; Scollard, Deborah A.; Kulbatski, Iris; Medeiros, Philip J; Sinisi, Riccardo; Dubikovskaya, Elena A.; Thomas J A Snoeks; van Beek, Ermond R.; Chan, Alan B.; Löwik, Clemens W. G. M.; DaCosta, Ralph S.

    2016-01-01

    textabstractPurpose: Most effective antitumor therapies induce tumor cell death. Non-invasive, rapid and accurate quantitative imaging of cell death is essential for monitoring early response to antitumor therapies. To facilitate this, we previously developed a biocompatible necrosis-avid near-infrared fluorescence (NIRF) imaging probe, HQ4, which was radiolabeled with 111Indium-chloride (111In-Cl3) via the chelate diethylene triamine pentaacetic acid (DTPA), to enable clinical translation. T...

  9. MR pulmonary ventilation information of canines inhaling aerosolized gadolinium-DTPA%应用雾化Gd DTPA获得犬肺通气信息

    Institute of Scientific and Technical Information of China (English)

    郭佑民; 吴晓明; 杨健; 王建国; 雷晓燕

    2003-01-01

    AIM:To investigate the effect of inhaling aerosolized gadolinium DTPA (Gd-DTPA) on pulmonary MR imaging in canines. METHODS:Six healthy northern dogs were performed MR scanning by using single shot turbo spin echo sequence.The changes of signal intensity (SI) in pulmonary parenchyma were respectively measured after inhaling air gas,aerosolized sodium chloride and Gd-DTPA as contrast agents.The differences of SI were compared between them. RESULTS:The SI of pulmonary parenchyma increased by 11.5%- 25.8% after inhalation of aerosolized sodium chloride solution, with no statistic difference compared to inhalation of air(t=2.798,P >0.05). Whereas the SI after inhalation of aerosolized Gd DTPA increased much higher by 42.7%- 76.8% than that after inhalation of air and aerosolized sodium chloride solution(t=4.660, P0.05);吸入 Gd-DTPA雾化颗粒后肺实质信号强度明显增加,增强幅度 42.7~ 76.8%,平均 59.2%,与吸入空气时的信号强度相比有统计学差异 (t=4.660,P< 0.05). 结论 :雾化的 Gd-DTPA可以作为一种有效的肺部 MRI增强对比剂,应用雾化 Gd DTPA来获得大型实验动物的有关肺通气信息是可行的.

  10. Evaluation of residual functional lung volume on Tc-99m DTPA aerosol ventilation and Tc-99m MAA perfusion scintigraphy in primary ciliary dyskinesia (Kartagener syndrome).

    Science.gov (United States)

    Chen, Yu-Wen; Chang, Chin-Chuan; Lai, Yung-Chuang; Lu, Chia-Ying; Dai, Zen-Kong

    2008-12-01

    Kartagener syndrome is diagnosed as sinusitis, bronchitis (bronchiectasis), and situs inversus by the clinical features. It is a subclass of primary ciliary dyskinesia (PCD) disease. A 12-year-old girl who had frequent upper and lower airway infections since birth, which was confirmed as Kartagener syndrome by HRCT imaging. We present the residual functional lung volume and mucociliary clearance findings seen on Tc-99m DTPA aerosol ventilation and Tc-99m MAA perfusion scintigraphy.

  11. Organic and inorganic fertilization effects on DTPA-extractable Fe, Cu, Mn and Zn, and their concentration in the edible portion of crops

    OpenAIRE

    Maqueda Porras, Celia; Herencia, Juan F.; Ruiz Porras, J. C.; Hidalgo García, Mª F.

    2011-01-01

    The application of organic composts to soil may affect the availability of micronutrients and their concentration in plants. The present field research study compared soil micronutrient extractability after 5 years of organic fertilization v. conventional inorganic fertilization. Iron (Fe), copper (Cu), manganese (Mn) and zinc (Zn) were extracted from soil using diethylene triamine pentaacetic acid (DTPA) and the data obtained were compared with the concentration of these micronutrients in th...

  12. Contribution of the hepatobiliary phase of Gd-EOB-DTPA-enhanced MRI to Dynamic MRI in the detection of hypovascular small ({<=}2 cm) HCC in cirrhosis

    Energy Technology Data Exchange (ETDEWEB)

    Golfieri, Rita; Renzulli, Matteo; Lucidi, Vincenzo; Corcioni, Beniamino [University of Bologna, Radiology Unit, Department of Digestive Diseases and Internal Medicine, Sant' Orsola-Malpighi Hospital, Bologna (Italy); Trevisani, Franco [University of Bologna, Unit of Semeiotica, Department of Digestive Diseases and Internal Medicine, Sant' Orsola-Malpighi Hospital, Bologna (Italy); Bolondi, Luigi [University of Bologna, Unit of Internal Medicine, Department of Digestive Diseases and Internal Medicine, Sant' Orsola-Malpighi Hospital, Bologna (Italy)

    2011-06-15

    To prospectively assess the additional value of the hepatobiliary (HB) phase of Gd-EOB-DTPA-MRI in identifying and characterising small ({<=}2 cm) hepatocellular carcinomas (HCCs) undetermined in dynamic phases alone because of their atypical features, according to the AASLD criteria. 127 cirrhotic patients were evaluated with Gd-EOB-DTPA-MRI in two sets: unenhanced and dynamic phases; unenhanced, dynamic and HB phases. Sixty-two out of 215 nodules (29%) were atypical in 42 patients (33%). 62 atypical nodules were reported at histology: high-grade dysplastic nodules (HGDN)/early HCC (n = 20), low-grade DN (LGDN) (n = 21), regenerative nodules (n = 17) and nodular regenerative hyperplasia (n = 4). The sensitivity, specificity, accuracy, positive and negative predictive value (PPV, NPV) were increased by the addition of the HB phase: 88.4-99.4%, 88-95%, 88-98.5%, 97-99%, and 65-97.5%, respectively. Twenty atypical nodules were malignant (32%), 19 of which were characterised only during the HB phase. The HB phase is 11% more sensitive in the classification of HGDN/early HCC than dynamic MRI, with an added value of 32.5% in the NPV. The high incidence (33%) of atypical nodules and their frequent malignancy (32%) suggest the widespread employment of Gd-EOB-DTPA-MRI in the follow-up of small nodules ({<=}2 cm) in cirrhosis. (orig.)

  13. Comparison of Gd-Bz-TTDA, Gd-EOB-DTPA, and Gd-BOPTA for dynamic MR imaging of the liver in rat models.

    Science.gov (United States)

    Jaw, Twei-Shiun; Chen, Shih-Hsien; Wang, Yun-Ming; Hsu, Jui-Sheng; Kuo, Yu-Ting; Chiu, Yen-Yu; Tsai, Kun-Bow; Hsieh, Tsyh-Jyi; Liu, Gin-Chung

    2012-03-01

    To evaluate the competitive potential of a new lipophilic paramagnetic complex, Gd-Bz-TTDA [4-benzyl-3,6,10-tri (carboxymethyl)-3,6,10-triazado-decanedioic acid] compared with two other commercially available MR hepatobiliary contrast agents, gadobenate dimeglumine (Gd-BOPTA) and gadoxetic acid (Gd-EOB-DTPA), dynamic MR imaging studies were performed on normal and hepatocellular carcinoma (HCC) rat models using a 1.5-Tesla MR scanner. The results indicate that normal rats that were injected with 0.1 mmol/kg Gd-Bz-TTDA showed significantly more intense and persistent liver enhancement than those that were injected with the same dose of Gd-EOB-DTPA or Gd-BOPTA. All of these agents showed similar enhancement patterns in the implanted HCC. The liver-lesion contrast-to-noise ratios were higher and more persistent in rats that were injected with Gd-Bz-TTDA. These results indicate that Gd-Bz-TTDA is comparable with the commercially available hepatobiliary agents, Gd-EOB-DTPA and Gd-BOPTA, and can result in more intense and prolonged liver enhancement while still providing better liver-lesion discrimination. These results warrant further large-scale studies.

  14. Application of classification trees for the qualitative differentiation of focal liver lesions suspicious for metastasis in gadolinium-EOB-DTPA-enhanced liver MR imaging

    Energy Technology Data Exchange (ETDEWEB)

    Schelhorn, J. [Sophien und Hufeland Klinikum, Weimar (Germany). Dept. of Radiology and Nuclear Medicine; Benndorf, M.; Dietzel, M.; Burmeister, H.P.; Kaiser, W.A.; Baltzer, P.A.T. [Jena Univ. (Germany). Inst. of Diagnostic and Interventional Radiology

    2012-09-15

    Purpose: To evaluate the diagnostic accuracy of qualitative descriptors alone and in combination for the classification of focal liver lesions (FLLs) suspicious for metastasis in gadolinium-EOB-DTPA-enhanced liver MR imaging. Materials and Methods: Consecutive patients with clinically suspected liver metastases were eligible for this retrospective investigation. 50 patients met the inclusion criteria. All underwent Gd-EOB-DTPA-enhanced liver MRI (T2w, chemical shift T1w, dynamic T1w). Primary liver malignancies or treated lesions were excluded. All investigations were read by two blinded observers (O1, O2). Both independently identified the presence of lesions and evaluated predefined qualitative lesion descriptors (signal intensities, enhancement pattern and morphology). A reference standard was determined under consideration of all clinical and follow-up information. Statistical analysis besides contingency tables (chi square, kappa statistics) included descriptor combinations using classification trees (CHAID methodology) as well as ROC analysis. Results: In 38 patients, 120 FLLs (52 benign, 68 malignant) were present. 115 (48 benign, 67 malignant) were identified by the observers. The enhancement pattern, relative SI upon T2w and late enhanced T1w images contributed significantly to the differentiation of FLLs. The overall classification accuracy was 91.3 % (O1) and 88.7 % (O2), kappa = 0.902. Conclusion: The combination of qualitative lesion descriptors proposed in this work revealed high diagnostic accuracy and interobserver agreement in the differentiation of focal liver lesions suspicious for metastases using Gd-EOB-DTPA-enhanced liver MRI. (orig.)

  15. Marginal erosive discovertebral ''Romanus'' lesions in ankylosing spondylitis demonstrated by contrast enhanced Gd-DTPA magnetic resonance imaging

    Energy Technology Data Exchange (ETDEWEB)

    Jevtic, V. [Clinical Radiology Institute, University Clinical Centre, Ljubljana (Slovenia); Kos-Golja, M.; Rozman, B. [Department of Rheumatology, University Clinical Centre, Ljubljana (Slovenia); McCall, I. [Robert Jones and Agnes Hunt Orthopaedic and District Hospital, Oswestry, Shropshire (United Kingdom)

    2000-01-01

    Objective. To assess the value of Gd-DTPA magnetic resonance (MR) imaging in the demonstration of marginal destructive discovertebral Romanus lesions in ankylosing spondylitis.Design and patients. A prospective study of Gd-DTPA MR imaging was performed in 39 patients with a clinical diagnosis of ankylosing spondylitis and typical Romanus lesions seen on radiographs of the thoracolumbar spine. MR morphological appearances and signal intensity changes at the discovertebral junctions were analysed and compared with the radiographic findings.Results. Ninety-nine discovertebral junctions with Romanus lesions showed low signal intensity on T1-weighted and high signal on T2-weighted and T1-weighted postcontrast images at the vertebral corners consistent with oedematous hyperaemic inflammatory tissue. There were nine discovertebral junctions with similar MR findings but normal radiographs. Fifty-three discovertebral junctions showed syndesmophyte formation with increased signal intensity on both T1- and T2-weighted images with no contrast enhancement. Sixty-five discovertebral junctions showed a mixture of radiographic features and varied high and low signal changes at the vertebral rim on MR imaging with rims of enhancement in the vertebral body following contrast administration.Conclusion. Gd-DTPA MR imaging demonstrates a variable signal pattern and degree of contrast enhancement which may reflect the evolutionary stages of discovertebral enthesitis in ankylosing spondylitis. MR imaging may identify early erosive changes in radiographically normal vertebra. The role of MR imaging needs further investigation. (orig.)

  16. Gd-DTPA-Dopamine-Bisphytanyl Amphiphile: Synthesis, Characterisation and Relaxation Parameters of the Nanoassemblies and Their Potential as MRI Contrast Agents.

    Science.gov (United States)

    Gupta, Abhishek; Willis, Scott A; Waddington, Lynne J; Stait-Gardner, Tim; de Campo, Liliana; Hwang, Dennis W; Kirby, Nigel; Price, William S; Moghaddam, Minoo J

    2015-09-28

    Here, a new amphiphilic magnetic resonance imaging (MRI) contrast agent, a Gd(III)-chelated diethylenetriaminepentaacetic acid conjugated to two branched alkyl chains via a dopamine spacer, Gd-DTPA-dopamine-bisphytanyl (Gd-DTPA-Dop-Phy), which is readily capable of self-assembling into liposomal nanoassemblies upon dispersion in an aqueous solution, is reported. In vitro relaxivities of the dispersions were found to be much higher than Magnevist, a commercially available contrast agent, at 0.47 T but comparable at 9.40 T. Analysis of variable temperature (17)O NMR transverse relaxation measurements revealed the water exchange of the nanoassemblies to be faster than that previously reported for paramagnetic liposomes. Molecular reorientation dynamics were probed by (1)H NMRD profiles using a classical inner and outer sphere relaxation model and a Lipari-Szabo "model-free" approach. High payloads of Gd(III) ions in the liposomal nanoassemblies made solely from the Gd-DTPA-Dop-Phy amphiphiles, in combination with slow molecular reorientation and fast water exchange makes this novel amphiphile a suitable candidate to be investigated as an advanced MRI contrast agent.

  17. Volume Change and Liver Parenchymal Signal Intensity in Gd-EOB-DTPA-Enhanced Magnetic Resonance Imaging after Portal Vein Embolization prior to Hepatectomy

    Directory of Open Access Journals (Sweden)

    Ayako Akiba

    2014-01-01

    Full Text Available Purpose. To investigate the liver volume change and the potential of early evaluation by contrast-enhanced magnetic resonance imaging (MRI using gadolinium-ethoxybenzyl-diethylenetriamine pentaacetic acid (Gd-EOB-DTPA after portal vein embolization (PVE. Materials and Methods. Retrospective evaluations of computed tomography (CT volumetry of total liver and nonembolized areas were performed before and 3 weeks after PVE in 37 cases. The percentage of future liver remnant (%FLR and the change ratio of %FLR (%FLR ratio were calculated. Prospective evaluation of signal intensities (SIs was performed to estimate the role of Gd-EOB-DTPA-enhanced MRI as a predictor of hypertrophy in 16 cases. The SI contrast between embolized and nonembolized areas was calculated 1 week after PVE. The change in SI contrast before and after PVE (SI ratio was also calculated in 11 cases. Results. %FLR ratio significantly increased, and SI ratio significantly decreased (both P<0.01. There were significant negative correlations between %FLR and SI contrast and between %FLR and SI ratio (both P<0.01. Conclusion. Hypertrophy in the nonembolized area after PVE was indicated by CT volumetry, and measurement of SI contrast and SI ratio in Gd-EOB-DTPA-enhanced MRI early after PVE may be useful to predict the potential for hepatic hypertrophy.

  18. Reversal of effects of intra peritoneally administered beryllium nitrate by tiron and CaNa3DTPA alone or in combination with alpha-tocopherol.

    Science.gov (United States)

    Nirala, Satendra Kumar; Bhadauria, Monika; Upadhyay, Anil Kumar; Mathur, Ramesh; Mathur, Asha

    2009-12-01

    To evaluate therapeutic efficacy of chelating agents tiron (Sodium-4,5-dihydroxy-1,3-benzene disulphonate) and CaNa3DTPA (Calcium trisodium diethylene triamine pentaacetic acid) in presence of alpha-tocopherol against beryllium induced toxicity, adult female albino rats were exposed to beryllium nitrate for 28 days followed by therapy with tiron (471 mg/kg, i.p.) and CaNa3DTPA (35 mg/kg, i.p.) alone and in combination with alpha-tocopherol (25 mg/kg, p.o.). Results revealed non-significant fall in haemoglobin and total serum protein content while significant fall in blood sugar level and activity of serum alkaline phosphatase. On the other hand, significant rise in the activity of serum transaminases and LDH was noticed after beryllium administration. Significant increase in total and esterified cholesterol was found in liver and kidney after toxicity. Significant increase in lipid peroxidation and decreased level of reduced glutathione in both the organs showed oxidative stress due to beryllium exposure. Histopathological and ultrastructural observations of liver and kidney revealed lesions due to beryllium toxicity followed by recovery due to combined therapy. CaNa3DTPA showed moderate therapeutic efficacy; however, its effectiveness was enhanced with alpha-tocopherol to some extent. Tiron in combination with alpha-tocopherol exerted statistically more beneficial effects in reversal of beryllium induced biochemical, histopathological and ultrastructural alterations.

  19. A generic probability based algorithm to derive regional patterns of crops in time and space

    Science.gov (United States)

    Wattenbach, Martin; Oijen, Marcel v.; Leip, Adrian; Hutchings, Nick; Balkovic, Juraj; Smith, Pete

    2013-04-01

    Croplands are not only the key to human food supply, they also change the biophysical and biogeochemical properties of the land surface leading to changes in the water cycle, energy partitioning, influence soil erosion and substantially contribute to the amount of greenhouse gases entering the atmosphere. The effects of croplands on the environment depend on the type of crop and the associated management which both are related to the site conditions, economic boundary settings as well as preferences of individual farmers. However, at a given point of time the pattern of crops in a landscape is not only determined by environmental and socioeconomic conditions but also by the compatibility to the crops which had been grown in the years before at the current field and its surrounding cropping area. The crop compatibility is driven by factors like pests and diseases, crop driven changes in soil structure and timing of cultivation steps. Given these effects of crops on the biochemical cycle and their interdependence with the mentioned boundary conditions, there is a demand in the regional and global modelling community to account for these regional patterns. Here we present a Bayesian crop distribution generator algorithm that is used to calculate the combined and conditional probability for a crop to appear in time and space using sparse and disparate information. The input information to define the most probable crop per year and grid cell is based on combined probabilities derived from the a crop transition matrix representing good agricultural practice, crop specific soil suitability derived from the European soil database and statistical information about harvested area from the Eurostat database. The reported Eurostat crop area also provides the target proportion to be matched by the algorithm on the level of administrative units (Nomenclature des Unités Territoriales Statistiques - NUTS). The algorithm is applied for the EU27 to derive regional spatial and

  20. A plant derived multifunctional tool for nanobiotechnology based on Tomato bushy stunt virus.

    Science.gov (United States)

    Grasso, Simone; Lico, Chiara; Imperatori, Francesca; Santi, Luca

    2013-06-01

    Structure, size, physicochemical properties and production strategies make many plant viruses ideal protein based nanoscaffolds, nanocontainers and nano-building blocks expected to deliver a multitude of applications in different fields such as biomedicine, pharmaceutical chemistry, separation science, catalytic chemistry, crop pest control and biomaterials science. Functionalization of viral nanoparticles through modification by design of their external and internal surfaces is essential to fully exploit the potentiality of these objects. In the present paper we describe the development of a plant derived multifunctional tool for nanobiotechnology based on Tomato bushy stunt virus. We demonstrate the ability of this system to remarkably sustain genetic modifications and in vitro chemical derivatizations of its outer surface, which resulted in the successful display of large chimeric peptides fusions and small chemical molecules, respectively. Moreover, we have defined physicochemical conditions for viral swelling and reversible viral pore gating that we have successfully employed for foreign molecules loading and retention in the inner cavity of this plant virus nanoparticles system. Finally, a production and purification strategy from Nicotiana benthamiana plants has been addressed and optimized.

  1. A novel acidic pH fluorescent probe based on a benzothiazole derivative.

    Science.gov (United States)

    Ma, Qiujuan; Li, Xian; Feng, Suxiang; Liang, Beibei; Zhou, Tiqiang; Xu, Min; Ma, Zhuoyi

    2017-04-15

    A novel acidic pH fluorescent probe 1 based on a benzothiazole derivative has been designed, synthesized and developed. The linear response range covers the acidic pH range from 3.44 to 6.46, which is valuable for pH researches in acidic environment. The evaluated pKa value of the probe 1 is 4.23. The fluorescence enhancement of the studied probe 1 with an increase in hydrogen ions concentration is based on the hindering of enhanced photo-induced electron transfer (PET) process. Moreover, the pH sensor possesses a highly selective response to H(+) in the presence of metal ions, anions and other bioactive small molecules which would be interfere with its fluorescent pH response. Furthermore, the probe 1 responds to acidic pH with short response time that was less than 1min. The probe 1 has been successfully applied to confocal fluorescence imaging in live HeLa cells and can selectively stain lysosomes. All of such good properties prove it can be used to monitoring pH fluctuations in acidic environment with high sensitivity, pH dependence and short response time.

  2. Synthesis of kaempferide Mannich base derivatives and their antiproliferative activity on three human cancer cell lines.

    Science.gov (United States)

    Nguyen, Van-Son; Shi, Ling; Luan, Fang-Qian; Wang, Qiu-An

    2015-01-01

    Kaempferide (3,5,7-trihydroxy-4'-methoxyflavone, 1), a naturally occurring flavonoid with potent anticancer activity in a number of human tumour cell lines, was first semisynthesized from naringin. Based on Mannich reaction of kaempferide with various secondary amines and formaldehyde, nine novel kaempferide Mannich base derivatives 2-10 were synthesized. The aminomethylation occurred preferentially in the position at C-6 and C-8 of the A-ring of kaempferide. All the synthetic compounds were tested for antiproliferative activity against three human cancer cell lines (Hela, HCC1954, SK-OV-3) by the standard MTT method. The results showed that compounds 1, 2 and 5-10 were more potent against Hela cells with IC50 values of 12.47-28.24 μM than the positive control cis-platin (IC50 41.25 μM), compounds 5, 6, 8 and 10 were more potent against HCC1954 cells with IC50 values of 8.82-14.97 μM than the positive control cis-platin (IC50 29.68 μM), and compounds 2, 3, 5, 6 and 10 were more potent against SK-OV-3 cells with IC50 values of 7.67-18.50 μM than the positive control cis-platin (IC50 21.27 μM).

  3. Semi-aromatic polyesters based on a carbohydrate-derived rigid diol for engineering plastics.

    Science.gov (United States)

    Wu, Jing; Eduard, Pieter; Thiyagarajan, Shanmugam; Noordover, Bart A J; van Es, Daan S; Koning, Cor E

    2015-01-01

    New carbohydrate-based polyesters were prepared from isoidide-2,5-dimethanol (extended isoidide, XII) through melt polymerization with dimethyl esters of terephthalic acid (TA) and furan-2,5-dicarboxylic acid (FDCA), yielding semi-crystalline prepolymers. Subsequent solid-state post-condensation (SSPC) gave high molecular weight (Mn =30 kg mol(-1) for FDCA) materials, the first examples of high Mn , semi-aromatic homopolyesters containing isohexide derivatives obtained via industrially relevant procedures. NMR spectroscopy showed that the stereo-configuration of XII was preserved under the applied conditions. The polyesters are thermally stable up to 380 °C. The TA- and FDCA-based polyesters have high Tg (105 °C and 94 °C, resp.) and Tm (284 °C and 250 °C, resp.) values. Its reactivity, stability, and ability to afford high Tg and Tm polyesters make XII a promising diol for the synthesis of engineering polymers.

  4. Evaluation of a physically-based snow model with infrared and microwave satellite-derived estimates

    Science.gov (United States)

    Wang, L.

    2013-05-01

    Snow (with high albedo, as well as low roughness and thermal conductivity) has significant influence on the land-atmosphere interactions in the cold climate and regions of high elevation. The spatial and temporal variability of the snow distribution on a basin scale greatly determines the timing and magnitude of spring snowmelt runoff. For improved water resources management, a physically-based distributed snow model has been developed and applied to the upper Yellow River Basin to provide the outputs of snow variables as well as streamflows from 2001 to 2005. Remotely-sensed infrared information from MODIS satellites has been used to evaluate the model's outputs of spatially-distributed snow cover extent (SCE) and land surface temperature (LST); while the simulated snow depth (SD) and snow water equivalent (SWE) have been compared with the microwave information from SSM/I and AMSR-E satellites. In general, the simulated streamflows (including spring snowmelt) agree fairly well with the gauge-based observations; while the modeled snow variables show acceptable accuracies through comparing to various satellite-derived estimates from infrared or microwave information.;

  5. QSAR modeling for quinoxaline derivatives using genetic algorithm and simulated annealing based feature selection.

    Science.gov (United States)

    Ghosh, P; Bagchi, M C

    2009-01-01

    With a view to the rational design of selective quinoxaline derivatives, 2D and 3D-QSAR models have been developed for the prediction of anti-tubercular activities. Successful implementation of a predictive QSAR model largely depends on the selection of a preferred set of molecular descriptors that can signify the chemico-biological interaction. Genetic algorithm (GA) and simulated annealing (SA) are applied as variable selection methods for model development. 2D-QSAR modeling using GA or SA based partial least squares (GA-PLS and SA-PLS) methods identified some important topological and electrostatic descriptors as important factor for tubercular activity. Kohonen network and counter propagation artificial neural network (CP-ANN) considering GA and SA based feature selection methods have been applied for such QSAR modeling of Quinoxaline compounds. Out of a variable pool of 380 molecular descriptors, predictive QSAR models are developed for the training set and validated on the test set compounds and a comparative study of the relative effectiveness of linear and non-linear approaches has been investigated. Further analysis using 3D-QSAR technique identifies two models obtained by GA-PLS and SA-PLS methods leading to anti-tubercular activity prediction. The influences of steric and electrostatic field effects generated by the contribution plots are discussed. The results indicate that SA is a very effective variable selection approach for such 3D-QSAR modeling.

  6. Size Switchable Supramolecular Nanoparticle Based on Azobenzene Derivative within Anionic Pillar[5]arene

    Science.gov (United States)

    Zhang, Cai-Cai; Li, Sheng-Hua; Zhang, Cui-Fang; Liu, Yu

    2016-11-01

    A photo/thermal-switchable supramolecular nanoparticles assembly has been constructed based on an inclusion complex between anionic pillar[5]arene 2C-WP5A and azobenzene derivative Azo-py-OMe (G). The novel anionic pillar[5]arene-based host-guest inclusion complexation was investigated by the 1H NMR titration, 2D ROESY and isothermal titration microcalorimetry (ITC) showing high association constant (Ka) of (2.60 ± 0.06) × 104 M‑1 with 1:1 binding stoichiometry. Furthermore, the supramolecular nanoparticles assembly can be conveniently obtained from G and a small amount of 2C-WP5A in aqueous solution, which was so-called “host induced aggregating (HIA)”. The size and morphology of the supramolecular nanoparticles assembly were characterized by TEM and DLS. As a result of the photo/thermal-isomerization of G included in the cavity of 2C-WP5A, the size of these nanoparticles could reversibly change from ~800 nm to ~250 nm, which could switch the solution of this assembly from turbid to clear.

  7. Discovery and development of natural product-derived chemotherapeutic agents based on a medicinal chemistry approach.

    Science.gov (United States)

    Lee, Kuo-Hsiung

    2010-03-26

    Medicinal plants have long been an excellent source of pharmaceutical agents. Accordingly, the long-term objectives of the author's research program are to discover and design new chemotherapeutic agents based on plant-derived compound leads by using a medicinal chemistry approach, which is a combination of chemistry and biology. Different examples of promising bioactive natural products and their synthetic analogues, including sesquiterpene lactones, quassinoids, naphthoquinones, phenylquinolones, dithiophenediones, neo-tanshinlactone, tylophorine, suksdorfin, DCK, and DCP, will be presented with respect to their discovery and preclinical development as potential clinical trial candidates. Research approaches include bioactivity- or mechanism of action-directed isolation and characterization of active compounds, rational drug design-based modification and analogue synthesis, and structure-activity relationship and mechanism of action studies. Current clinical trial agents discovered by the Natural Products Research Laboratories, University of North Carolina, include bevirimat (dimethyl succinyl betulinic acid), which is now in phase IIb trials for treating AIDS. Bevirimat is also the first in a new class of HIV drug candidates called "maturation inhibitors". In addition, an etoposide analogue, GL-331, progressed to anticancer phase II clinical trials, and the curcumin analogue JC-9 is in phase II clinical trials for treating acne and in development for trials against prostate cancer. The discovery and development of these clinical trial candidates will also be discussed.

  8. Deriving Shape-Based Features for C. elegans Locomotion Using Dimensionality Reduction Methods

    Science.gov (United States)

    Gyenes, Bertalan; Brown, André E. X.

    2016-01-01

    High-throughput analysis of animal behavior is increasingly common following the advances of recording technology, leading to large high-dimensional data sets. This dimensionality can sometimes be reduced while still retaining relevant information. In the case of the nematode worm Caenorhabditis elegans, more than 90% of the shape variance can be captured using just four principal components. However, it remains unclear if other methods can achieve a more compact representation or contribute further biological insight to worm locomotion. Here we take a data-driven approach to worm shape analysis using independent component analysis (ICA), non-negative matrix factorization (NMF), a cosine series, and jPCA (a dynamic variant of principal component analysis [PCA]) and confirm that the dimensionality of worm shape space is close to four. Projecting worm shapes onto the bases derived using each method gives interpretable features ranging from head movements to tail oscillation. We use these as a comparison method to find differences between the wild type N2 worms and various mutants. For example, we find that the neuropeptide mutant nlp-1(ok1469) has an exaggerated head movement suggesting a mode of action for the previously described increased turning rate. The different bases provide complementary views of worm behavior and we expect that closer examination of the time series of projected amplitudes will lead to new results in the future. PMID:27582697

  9. Structure and physicochemical properties of thin film photosemiconductor cells based on porphine derivatives

    Science.gov (United States)

    Kazak, A. V.; Usol'tseva, N. V.; Smirnova, A. I.; Bodnarchuk, V. V.; Sul'yanov, S. N.; Yablonskii, S. V.

    2016-05-01

    Photosemiconductor thin films based on two organic porphine derivatives have been investigated. These compounds have different pendent groups; the film morphology, along with the specific fabrication technique, is determined to a great extent by these groups. The films have been fabricated by vacuum sputtering and using the Langmuir-Schaefer method. According to the atomic force microscopy (AFM) data, the Langmuir-Schaefer films are more homogeneous than the sputtered ones. It is shown that the sputtered films based on substituted porphine have a looser stacking than the initial analog. A spectroscopy study revealed a bathochromic shift of the Soret band in the Langmuir-Schaefer films-sputtered films series. This shift is explained by the increase in the concentration and size of molecular aggregates in sputtered films. It is shown that a polycrystalline C60 fullerene film deposited onto an amorphous substituted porphine layer improves the photoelectric characteristics of the latter. Both the time stability of the photodiode structure and its ampere‒watt sensitivity increase (by a factor of 10 in the transition regime). The steady-state current does not change. The effect of polarity reversal of the photovoltaic signal is observed in a planar C60‒substituted metalloporphine heterostructure, which is similar to the pyroelectric effect. The polarity reversal can be explained by the contribution of the trap charge and discharge current at the interface between the amorphous photosemiconductor and crystalline photosemiconductor to the resulting photoelectric current.

  10. Barotropic Mechanisms of Derivative-based Uncertainty Propagation in Drake Passage Transport Estimation

    Science.gov (United States)

    Kalmikov, A.; Heimbach, P.

    2013-12-01

    We apply derivative-based uncertainty quantification (UQ) and sensitivity methods to the estimation of Drake Passage transport in a global barotropic configuration of the MIT ocean general circulation model (MITgcm). Sensitivity and uncertainty fields are evaluated via first and second derivative codes of the MITgcm, generated via algorithmic differentiation (AD). Observation uncertainties are projected to uncertainties in the control variables by inversion of the Hessian of the nonlinear least-squares misfit function. Only data-supported components of Hessian information are retained through elimination of the unconstrained uncertainty nullspace. The assimilated observation uncertainty is combined with prior control variable uncertainties to reduce their posterior uncertainty. The spatial patterns of posterior uncertainty reduction and their temporal evolution are explained in terms of barotropic dynamics. Global uncertainty teleconnection mechanisms are identified as barotropic uncertainty waves. Uncertainty coupling across different control fields is demonstrated by assimilation of sea surface height uncertainty. A second step in our UQ scheme consists in propagating prior and posterior uncertainties of the model controls onto model output variables of interest, here Drake Passage transport. Forward uncertainty propagation amounts to matrix transformation of the uncertainty covariances via the model Jacobian and its adjoint. Sources of uncertainties of the transport are revealed through analysis of the adjoint wave dynamics in the model. These adjoint (reversed) mechanisms are associated with the evolution of sensitivity fields and our method formally extends sensitivity analysis to uncertainty quantification. Inverse uncertainty propagation mechanisms can be linked to adjoint dynamics in a similar manner. The posterior correlations of controls are found to dominate the reduction of the transport uncertainty compared to the marginal uncertainty reduction of the

  11. Catalytic wet air oxidation of 2-chlorophenol over sewage sludge-derived carbon-based catalysts

    Energy Technology Data Exchange (ETDEWEB)

    Tu, Yuting [Institut de recherches sur la catalyse et l’environnement de Lyon (IRCELYON), CNRS – Université Claude Bernard Lyon 1, 2 Avenue Albert Einstein, 69626 Villeurbanne Cedex (France); School of Environmental Science and Engineering, Sun Yat-Sen University, Guangzhou 510275 (China); Xiong, Ya; Tian, Shuanghong [School of Environmental Science and Engineering, Sun Yat-Sen University, Guangzhou 510275 (China); Guangdong Provincial Key Laboratory of Environmental Pollution Control and Remediation Technology, Guangzhou 510275 (China); Kong, Lingjun [School of Environmental Science and Engineering, Sun Yat-Sen University, Guangzhou 510275 (China); Descorme, Claude, E-mail: claude.descorme@ircelyon.univ-lyon1.fr [Institut de recherches sur la catalyse et l’environnement de Lyon (IRCELYON), CNRS – Université Claude Bernard Lyon 1, 2 Avenue Albert Einstein, 69626 Villeurbanne Cedex (France)

    2014-07-15

    Highlights: • A sewage sludge derived carbon-supported iron oxide catalyst (FeSC) was prepared. • FeSC exhibited high catalytic activity in the wet air oxidation of 2-chlorophenol. • A strong correlation was observed between the 2-CP conversion, the iron leaching and the pH. • Using an acetate buffer, the iron leaching was suppressed while keeping some catalytic activity. • A simplified reaction pathway was proposed for the CWAO of 2-CP over the FeSC catalyst. - Abstract: A sewage sludge derived carbon-supported iron oxide catalyst (FeSC) was prepared and used in the Catalytic Wet Air Oxidation (CWAO) of 2-chlorophenol (2-CP). The catalysts were characterized in terms of elemental composition, surface area, pH{sub PZC}, XRD and SEM. The performances of the FeSC catalyst in the CWAO of 2-CP was assessed in a batch reactor operated at 120 °C under 0.9 MPa oxygen partial pressure. Complete decomposition of 2-CP was achieved within 5 h and 90% Total Organic Carbon (TOC) was removed after 24 h of reaction. Quite a straight correlation was observed between the 2-CP conversion, the amount of iron leached in solution and the pH of the reaction mixture at a given reaction time, indicating a strong predominance of the homogeneous catalysis contribution. The iron leaching could be efficiently prevented when the pH of the solution was maintained at values higher than 4.5, while the catalytic activity was only slightly reduced. Upon four successive batch CWAO experiments, using the same FeSC catalyst recovered by filtration after pH adjustment, only a very minor catalyst deactivation was observed. Finally, based on all the identified intermediates, a simplified reaction pathway was proposed for the CWAO of 2-CP over the FeSC catalyst.

  12. Large-scale derived flood frequency analysis based on continuous simulation

    Science.gov (United States)

    Dung Nguyen, Viet; Hundecha, Yeshewatesfa; Guse, Björn; Vorogushyn, Sergiy; Merz, Bruno

    2016-04-01

    There is an increasing need for spatially consistent flood risk assessments at the regional scale (several 100.000 km2), in particular in the insurance industry and for national risk reduction strategies. However, most large-scale flood risk assessments are composed of smaller-scale assessments and show spatial inconsistencies. To overcome this deficit, a large-scale flood model composed of a weather generator and catchments models was developed reflecting the spatially inherent heterogeneity. The weather generator is a multisite and multivariate stochastic model capable of generating synthetic meteorological fields (precipitation, temperature, etc.) at daily resolution for the regional scale. These fields respect the observed autocorrelation, spatial correlation and co-variance between the variables. They are used as input into catchment models. A long-term simulation of this combined system enables to derive very long discharge series at many catchment locations serving as a basic for spatially consistent flood risk estimates at the regional scale. This combined model was set up and validated for major river catchments in Germany. The weather generator was trained by 53-year observation data at 528 stations covering not only the complete Germany but also parts of France, Switzerland, Czech Republic and Australia with the aggregated spatial scale of 443,931 km2. 10.000 years of daily meteorological fields for the study area were generated. Likewise, rainfall-runoff simulations with SWIM were performed for the entire Elbe, Rhine, Weser, Donau and Ems catchments. The validation results illustrate a good performance of the combined system, as the simulated flood magnitudes and frequencies agree well with the observed flood data. Based on continuous simulation this model chain is then used to estimate flood quantiles for the whole Germany including upstream headwater catchments in neighbouring countries. This continuous large scale approach overcomes the several

  13. Silver ion recognition using potentiometric sensor based on recently synthesized isoquinoline-1,3-dione derivatives

    Directory of Open Access Journals (Sweden)

    AJAR KAMAL

    2012-08-01

    Full Text Available The four derivatives of isoquinoline-1,3-dione based on β-lactum (I-IV, have been explored as neutral ionophores for preparing poly(vinylchloride based polymeric membrane electrodes (PME selective to silver(I ions. The addition of sodium tetraphenylborate (NaTPB and dioctylsebacate (DOS as a plasticizer was found to improve the performance of ion selective electrodes. The best performance was obtained with PME-1 based on ionophore I having composition: ionophore (9.2 mg, PVC (100.1 mg, DOS (201.1 mg and NaTPB (1.5 mg in 5 mL tetrahydrofuran. The electrode response was linear with Nernstian slope of 58.44 mV/decade in the concentration range of 1.0 x 10-1 M to 5.0 x 10-6 M and detection limit of 5.83 x 10-6 M. It performs satisfactorily over wide pH range of 1.0-5.5. The proposed sensor can be used over a period of more than three months without any significant drift in potential and shows good selectivity to silver(I ion over a number of cations especially with no interference of mercury(II ions. Sharp end point was obtained when the sensor was used as an indicator electrode for the potentiometric titration of silver(I ions with chloride ions and therefore this electrode (PME-1 could be used for quantitative determination of silver(I ion in synthetic water, silver foil and dental amalgam samples.

  14. Design of two-channel filter bank using nature inspired optimization based fractional derivative constraints.

    Science.gov (United States)

    Kuldeep, B; Singh, V K; Kumar, A; Singh, G K

    2015-01-01

    In this article, a novel approach for 2-channel linear phase quadrature mirror filter (QMF) bank design based on a hybrid of gradient based optimization and optimization of fractional derivative constraints is introduced. For the purpose of this work, recently proposed nature inspired optimization techniques such as cuckoo search (CS), modified cuckoo search (MCS) and wind driven optimization (WDO) are explored for the design of QMF bank. 2-Channel QMF is also designed with particle swarm optimization (PSO) and artificial bee colony (ABC) nature inspired optimization techniques. The design problem is formulated in frequency domain as sum of L2 norm of error in passband, stopband and transition band at quadrature frequency. The contribution of this work is the novel hybrid combination of gradient based optimization (Lagrange multiplier method) and nature inspired optimization (CS, MCS, WDO, PSO and ABC) and its usage for optimizing the design problem. Performance of the proposed method is evaluated by passband error (ϕp), stopband error (ϕs), transition band error (ϕt), peak reconstruction error (PRE), stopband attenuation (As) and computational time. The design examples illustrate the ingenuity of the proposed method. Results are also compared with the other existing algorithms, and it was found that the proposed method gives best result in terms of peak reconstruction error and transition band error while it is comparable in terms of passband and stopband error. Results show that the proposed method is successful for both lower and higher order 2-channel QMF bank design. A comparative study of various nature inspired optimization techniques is also presented, and the study singles out CS as a best QMF optimization technique.

  15. A knowledge-based scoring function for protein-RNA interactions derived from a statistical mechanics-based iterative method.

    Science.gov (United States)

    Huang, Sheng-You; Zou, Xiaoqin

    2014-04-01

    Protein-RNA interactions play important roles in many biological processes. Given the high cost and technique difficulties in experimental methods, computationally predicting the binding complexes from individual protein and RNA structures is pressingly needed, in which a reliable scoring function is one of the critical components. Here, we have developed a knowledge-based scoring function, referred to as ITScore-PR, for protein-RNA binding mode prediction by using a statistical mechanics-based iterative method. The pairwise distance-dependent atomic interaction potentials of ITScore-PR were derived from experimentally determined protein-RNA complex structures. For validation, we have compared ITScore-PR with 10 other scoring methods on four diverse test sets. For bound docking, ITScore-PR achieved a success rate of up to 86% if the top prediction was considered and up to 94% if the top 10 predictions were considered, respectively. For truly unbound docking, the respective success rates of ITScore-PR were up to 24 and 46%. ITScore-PR can be used stand-alone or easily implemented in other docking programs for protein-RNA recognition.

  16. Inulin Derivatives Obtained Via Enhanced Microwave Synthesis for Nucleic Acid Based Drug Delivery.

    Science.gov (United States)

    Sardo, Carla; Craparo, Emanuela Fabiola; Fiorica, Calogero; Giammona, Gaetano; Cavallaro, Gennara

    2015-01-01

    A new class of therapeutic agents with a high potential for the treatment of different socially relevant human diseases is represented by Nucleic Acid Based Drugs (NABD), including small interfering RNAs (siRNA), decoy oligodeoxynucleotides (decoy ODN) and antisense oligonucleotides (ASOs). Although NABD can be engineered to be specifically directed against virtually any target, their susceptibility to nuclease degradation and the difficulty of delivery into target tissues severely limit their use in clinical practice and require the development of an appropriate nanostructured delivery system. For delivery of NABD, Inulin (Inu), a natural, water soluble and biocompatible polysaccharide, was derivatized by Spermine (Spm), a flexible molecule with four amine groups that, having pKa values in the range between 8-11, is mainly in the protonated form at pH 7.4. The synthesis of related copolymers (Inu-Spm) was performed by a two step reaction, using a method termed Enhanced Microwave Synthesis (EMS) which has the advantage, compared to conventional microwave reaction, that high amount of energy can be applied to the reaction system, by administering microwave irradiation and simultaneously controlling the temperature in the reaction vessel with cooled air. The synthesized inulin derivatives were characterized by FT-IR spectra and (1)H-NMR. INU-Spm derivatives with a degree of derivatization of about 14 % mol/mol were obtained. These polycations were tested to evaluate their ability to form non covalent complexes with genetic material (polyplexes). Agarose gel retardation assays showed that the obtained copolymers are able to electrostatically interact with DNA duplex to form polyplexes at different c/p weight ratios. Moreover, light scattering studies, performed to analyze size and z-potential of polyplexes, evidenced that copolymers are able to interact with genetic material leading to the formation of nanoscaled systems. In addition, biocompatibility of polyplexes

  17. A fungal phylogeny based on 42 complete genomes derived from supertree and combined gene analysis

    Directory of Open Access Journals (Sweden)

    Stajich Jason E

    2006-11-01

    Full Text Available Abstract Background To date, most fungal phylogenies have been derived from single gene comparisons, or from concatenated alignments of a small number of genes. The increase in fungal genome sequencing presents an opportunity to reconstruct evolutionary events using entire genomes. As a tool for future comparative, phylogenomic and phylogenetic studies, we used both supertrees and concatenated alignments to infer relationships between 42 species of fungi for which complete genome sequences are available. Results A dataset of 345,829 genes was extracted from 42 publicly available fungal genomes. Supertree methods were employed to derive phylogenies from 4,805 single gene families. We found that the average consensus supertree method may suffer from long-branch attraction artifacts, while matrix representation with parsimony (MRP appears to be immune from these. A genome phylogeny was also reconstructed from a concatenated alignment of 153 universally distributed orthologs. Our MRP supertree and concatenated phylogeny are highly congruent. Within the Ascomycota, the sub-phyla Pezizomycotina and Saccharomycotina were resolved. Both phylogenies infer that the Leotiomycetes are the closest sister group to the Sordariomycetes. There is some ambiguity regarding the placement of Stagonospora nodurum, the sole member of the class Dothideomycetes present in the dataset. Within the Saccharomycotina, a monophyletic clade containing organisms that translate CTG as serine instead of leucine is evident. There is also strong support for two groups within the CTG clade, one containing the fully sexual species Candida lusitaniae, Candida guilliermondii and Debaryomyces hansenii, and the second group containing Candida albicans, Candida dubliniensis, Candida tropicalis, Candida parapsilosis and Lodderomyces elongisporus. The second major clade within the Saccharomycotina contains species whose genomes have undergone a whole genome duplication (WGD, and their close

  18. Synthesis of Urea based Chalcone Derivatives and Evaluate its Biological Activity

    Directory of Open Access Journals (Sweden)

    Arpita Desai

    2016-05-01

    Full Text Available Chalcones have been the center of attraction for researchers from several decades due to nits innumerous therapeutic application, Efforts have been done in my research to synthesized chalcones and their derivatives that further reacts with various substituted aldehyde to give corresponding substituted chalcone derivatives. Now these derivatives on condensation with Guanidine nitrate gives the vast range of phenyl pyrimidine amine Derivatives. Structure elucidation of synthesized compound had been made on the basis of element analysis, 1H NMR Spectra studies. The microbial activity of the synthesized compounds has been studied against the species bacillus subtillis, staphylococcus aureus, Escherichia coli, and salmonella typhi.

  19. [Study on the characters of phytoplankton chlorophyll fluorescence excitation spectra based on fourth-derivative].

    Science.gov (United States)

    Lu, Lu; Su, Rong-Guo; Wang, Xiu-Lin; Zhu, Chen-Jian

    2007-11-01

    Chlorophyll fluorescence excitation spectra of six phytoplankton species, belonging to Bacillariophyta and Dinophyta, were dealt by fourth-derivative analysis with the Matlab program. The results show that between 350 nm and 550 nm six fluorescence peaks were found in the fourth-derivative spectra, which are representatives of non-pigments, chlorophylls and carotenoides respectively. The method makes Bacillariophyta and Dinophyta more distinguishable when the fourth-derivative spectra are compared with the chlorophyll fluorescence excitation spectra. It can be used not only to discriminate the two groups of algaes, but also to reduce the effect of noise. The fluorescence peaks in the fourth-derivative spectra are proved to be stable.

  20. Informative value of MR tomography using gadolinium-DTPA in the diagnosis of the lumbar post-discotomy syndrome

    Energy Technology Data Exchange (ETDEWEB)

    Beyer, H.K.; Hoetzinger, H.; Toedt, C.; Oppel, U.

    1989-03-01

    Our results have shown that MR tomography - especially if gadolinium-DTPA is used as a contrast medium - is an excellent method to differentiate between the various possible causes of the post-discotomy syndrome. In particular, it is definitely possible to differentiate between a relapsed hernia of an intervertebral dis and a postoperative development of cicatricial tissue which is practically inevitable. This differentiation is of vital importance to the clinician, because a second operation should be performed only in case of a relapse, whereas cicatricial tissue is best left alone, because no matter how carefully the operation is performed with preparation of the neural structures, even more extensive cicatricial plates are bound to develop. Instability of the vertebral column in the operated disc segment, or other causes of complaints, can be identified via MR equally well if not better than by other means. Hence, the method should be the method of choice in postdiscotomy syndrome, since it facilitates the difficult choice between conservative therapy and reoperation.

  1. The biodistribution and dosimetry of {sup 117m}Sn DTPA with special emphasis on active marrow absorbed doses

    Energy Technology Data Exchange (ETDEWEB)

    Stubbs, J. [Radiation Dosimetry Systems of Oak Ridge Inc., Knoxville, TN (United States); Atkins, H. [Brookhaven National Lab., Upton, NY (United States)

    1999-01-01

    {sup 117m}Sn(4+) DTPA is a new radiopharmaceutical for the palliation of pain associated with metastatic bone cancer. Recently, the Phase 2 clinical trials involving 47 patients were completed. These patients received administered activities in the range 6.7--10.6 MBq/kg of body mass. Frequent collections of urine were acquired over the first several hours postadministration and daily cumulative collections were obtained for the next 4--10 days. Anterior/posterior gamma camera images were obtained frequently over the initial 10 days. Radiation dose estimates were calculated for 8 of these patients. Each patient`s biodistribution data were mathematically simulated using a multicompartmental model. The model consisted of the following compartments: central, bone, kidney, other tissues, and cumulative urine. The measured cumulative urine data were used as references for the cumulative urine excretion compartment. The total-body compartment (sum of the bone surfaces, central, kidney, and other tissues compartments) was reference to all activity not excreted in the urine.

  2. Evaluation of methods to derive green-up dates based on daily NDVI satellite observations

    Science.gov (United States)

    Doktor, Daniel

    2010-05-01

    Bridging the gap between satellite derived green-up dates and in situ phenological observations has been the purpose of many studies over the last decades. Despite substantial advancements in satellite technology and data quality checks there is as yet no universally accepted method for extracting phenological metrics based on satellite derived vegetation indices. Dependent on the respective method derived green-up dates can vary up to serveral weeks using identical data sets. Consequently, it is difficult to compare various studies and to accurately determine an increased vegetation length due to changing temperature patterns as observed by ground phenological networks. Here, I compared how the characteristic NDVI increase over temperate deciduous forests in Germany in spring relates to respective budburst events observed on the ground. MODIS Terra daily surface reflectances with a 250 m resolution (2000-2008) were gathered to compute daily NDVI values. As ground truth, observations of the extensive phenological network of the German Weather Service were used. About 1500 observations per year and species (Beech, Oak and Birch) were available evenly distributed all over Germany. Two filtering methods were tested to reduce the noisy raw data. The first method only keeps NDVI values which are classified as ‚ideal global quality' and applies on those a temporal moving window where values are removed which differ more than 20% of the mean. The second method uses an adaptation of the BISE (Best Index Slope Extraction) algorithm. Subsequently, three functions were fitted to the selected observations: a simple linear interpolation, a sigmoidal function and a double logistic sigmoidal function allowing to approximate two temporally separated green-up signals. The green-up date was then determined at halfway between minimum and maximum (linear interpolation) or at the inflexion point of the sigmoidal curve. A number of global threshold values (NDVI 0.4,0.5,0.6) and

  3. Catalytic wet air oxidation of 2-chlorophenol over sewage sludge-derived carbon-based catalysts.

    Science.gov (United States)

    Tu, Yuting; Xiong, Ya; Tian, Shuanghong; Kong, Lingjun; Descorme, Claude

    2014-07-15

    A sewage sludge derived carbon-supported iron oxide catalyst (FeSC) was prepared and used in the Catalytic Wet Air Oxidation (CWAO) of 2-chlorophenol (2-CP). The catalysts were characterized in terms of elemental composition, surface area, pHPZC, XRD and SEM. The performances of the FeSC catalyst in the CWAO of 2-CP was assessed in a batch reactor operated at 120°C under 0.9MPa oxygen partial pressure. Complete decomposition of 2-CP was achieved within 5h and 90% Total Organic Carbon (TOC) was removed after 24h of reaction. Quite a straight correlation was observed between the 2-CP conversion, the amount of iron leached in solution and the pH of the reaction mixture at a given reaction time, indicating a strong predominance of the homogeneous catalysis contribution. The iron leaching could be efficiently prevented when the pH of the solution was maintained at values higher than 4.5, while the catalytic activity was only slightly reduced. Upon four successive batch CWAO experiments, using the same FeSC catalyst recovered by filtration after pH adjustment, only a very minor catalyst deactivation was observed. Finally, based on all the identified intermediates, a simplified reaction pathway was proposed for the CWAO of 2-CP over the FeSC catalyst.

  4. Derivation of an observation-based map of North African dust emission

    Energy Technology Data Exchange (ETDEWEB)

    Evan, Amato T.; Fiedler, Stephanie; Zhao, Chun; Menut, Laurent; Schepanski, Kerstin; Flamant, C.; Doherty, Owen

    2015-03-01

    Changes in the emission, transport and deposition of aeolian dust have profound effects on regional climate, so that characterizing the lifecycle of dust in observations and improving the representation of dust in global climate models is necessary. A fundamental aspect of characterizing the dust cycle is quantifying surface dust fluxes, yet no spatially explicit estimates of this flux exist for the World’s major source regions. Here we present a novel technique for creating a map of the annual mean emitted dust flux for North Africa based on retrievals of dust storm frequency from the Meteosat Second Generation Spinning Enhanced Visible and InfraRed Imager (SEVIRI) and the relationship between dust storm frequency and emitted mass flux derived from the output of five models that simulate dust. Our results suggest that 64 (±16)% of all dust emitted from North Africa is from the Bodélé depression, and that 13 (±3)% of the North African dust flux is from a depression lying in the lee of the Aïr and Hoggar Mountains, making this area the second most important region of emission within North Africa.

  5. Synthesis, Spectroscopic Characterization and Biological Activities of Transition Metal Complexes Derived from a Tridentate Schiff Base

    Directory of Open Access Journals (Sweden)

    J. Senthil Kumaran

    2013-01-01

    Full Text Available A new series of Cu (II, Ni (II, Co (II and Zn (II complexes have been synthesized from the Schiff base derived from 4-hydroxy-3-methoxybenzylidine-4-aminoantipyrine and 2-aminophenol. The structural features have been determined from their elemental analysis, magnetic susceptibility, molar conductance, Mass, IR, UV-Vis, 1H-NMR, 13C-NMR and ESR spectral studies. The redox behavior of the copper complex has been studied by cyclic voltammetry. The data confirm that the complexes have composition of ML2 type. The electronic absorption spectral data of the complexes propose an octahedral geometry around the central metal ion. All the metal complexes with DNA structure were guided by the presence of inter-molecular C–H⋯O and C–H⋯N hydrogen bonds. The biological activity of the synthesized compounds were tested against the bacterial species such as Bacillus subtilis, Staphylococcus aureus, Proteus vulgaris and fungal species such as Candida albicans by the well-diffusion method.

  6. Satellite-Based Derivation of High-Resolution Forest Information Layers for Operational Forest Management

    Directory of Open Access Journals (Sweden)

    Johannes Stoffels

    2015-06-01

    Full Text Available A key factor for operational forest management and forest monitoring is the availability of up-to-date spatial information on the state of forest resources. Earth observation can provide valuable contributions to these information needs. The German federal state of Rhineland-Palatinate transferred its inherited forest information system to a new architecture that is better able to serve the needs of centralized inventory and planning services, down to the level of forest districts. During this process, a spatially adaptive classification approach was developed to derive high-resolution forest information layers (e.g., forest type, tree species distribution, development stages based on multi-temporal satellite data. This study covers the application of the developed approach to a regional scale (federal state level and the further adaptation of the design to meet the information needs of the state forest service. The results confirm that the operational requirements for mapping accuracy can, in principle, be fulfilled. However, the state-wide mapping experiment also revealed that the ability to meet the required level of accuracy is largely dependent on the availability of satellite observations within the optimum phenological time-windows.

  7. Dynamic Covalent Chemistry-based Sensing: Pyrenyl Derivatives of Phenylboronic Acid for Saccharide and Formaldehyde

    Science.gov (United States)

    Chang, Xingmao; Fan, Jiayun; Wang, Min; Wang, Zhaolong; Peng, Haonan; He, Gang; Fang, Yu

    2016-01-01

    We synthesized two specially designed pyrenyl (Py) derivatives of phenylboronic acid, PSNB1 and PSNB2, of which PSNB2 self-assemble to form dynamic aggregate in methanol-water mixture (1:99, v/v) via intermolecular H-bonding and pi-pi stacking. Interestingly, the dynamic aggregate shows smart response to presence of fructose (F) as evidenced by fluorescence color change from green to blue. More interestingly, the fluorescence emission of the resulted PSNB2-F changes from blue to green with the addition of formaldehyde (FA). The reason behind is formation of a PSNB2-F dimer via FA cross-linking. Based upon the reactions as found, sensitive and fast sensing of F and FA in water was realized, of which the experimental DLs could be significantly lower than 10 μM for both analytes, and the response times are less than 1 min. It is believed that not only the materials as created may have the potential to find real-life applications but also the strategy as developed can be adopted to develop other dynamic materials. PMID:27498703

  8. Dynamic Covalent Chemistry-based Sensing: Pyrenyl Derivatives of Phenylboronic Acid for Saccharide and Formaldehyde

    Science.gov (United States)

    Chang, Xingmao; Fan, Jiayun; Wang, Min; Wang, Zhaolong; Peng, Haonan; He, Gang; Fang, Yu

    2016-08-01

    We synthesized two specially designed pyrenyl (Py) derivatives of phenylboronic acid, PSNB1 and PSNB2, of which PSNB2 self-assemble to form dynamic aggregate in methanol-water mixture (1:99, v/v) via intermolecular H-bonding and pi-pi stacking. Interestingly, the dynamic aggregate shows smart response to presence of fructose (F) as evidenced by fluorescence color change from green to blue. More interestingly, the fluorescence emission of the resulted PSNB2-F changes from blue to green with the addition of formaldehyde (FA). The reason behind is formation of a PSNB2-F dimer via FA cross-linking. Based upon the reactions as found, sensitive and fast sensing of F and FA in water was realized, of which the experimental DLs could be significantly lower than 10 μM for both analytes, and the response times are less than 1 min. It is believed that not only the materials as created may have the potential to find real-life applications but also the strategy as developed can be adopted to develop other dynamic materials.

  9. Synthesis and Evaluation of New Benzodioxole- Based Thiosemicarbazone Derivatives as Potential Antitumor Agents

    Directory of Open Access Journals (Sweden)

    Mehlika Dilek Altıntop

    2016-11-01

    Full Text Available New benzodioxole-based thiosemicarbazone derivatives were synthesized and evaluated for their cytotoxic effects on A549 human lung adenocarcinoma, C6 rat glioma and NIH/3T3 mouse embryonic fibroblast cells. In order to examine the correlation between anticancer activity and cholinesterases, the compounds were evaluated for their inhibitory effects on AChE and BuChE. The most effective anticancer agents were investigated for their effects on DNA synthesis, apoptosis and mitochondrial membrane potential. 4-(1,3-Benzodioxol-5-yl-1-([1,1′-biphenyl]-4-ylmethylenethiosemicarbazide (5 was identified as the most promising anticancer agent against C6 and A549 cell lines due to its inhibitory effects on C6 and A549 cells and low toxicity to NIH/3T3 cells. Compound 5 increased early and late apoptosis in A549 and C6 cells. Compound 5 also caused disturbance on mitochondrial membrane potential and showed DNA synthesis inhibitory activity in A549 and C6 cells. Compound 5 was investigated for SIRT1 inhibitory activity to provide mechanistic insight and for that purpose docking studies were also performed for this compound on SIRT1. On the other hand, compound 5 did not show any inhibitory activity against AChE and BuChE. This outcome pointed out that there is no relationship between anticancer activity of compound 5 and cholinesterases.

  10. Novel Organotin(IV) Schiff Base Complexes with Histidine Derivatives: Synthesis, Characterization, and Biological Activity

    Science.gov (United States)

    Garza-Ortiz, Ariadna; Camacho-Camacho, Carlos; Sainz-Espuñes, Teresita; Rojas-Oviedo, Irma; Gutiérrez-Lucas, Luis Raúl; Gutierrez Carrillo, Atilano; Vera Ramirez, Marco A.

    2013-01-01

    Five novel tin Schiff base complexes with histidine analogues (derived from the condensation reaction between L-histidine and 3,5-di-tert-butyl-2-hydroxybenzaldehyde) have been synthesized and characterized. Characterization has been completed by IR and high-resolution mass spectroscopy, 1D and 2D solution NMR (1H, 13C  and 119Sn), as well as solid state 119Sn NMR. The spectroscopic evidence shows two types of structures: a trigonal bipyramidal stereochemistry with the tin atom coordinated to five donating atoms (two oxygen atoms, one nitrogen atom, and two carbon atoms belonging to the alkyl moieties), where one molecule of ligand is coordinated in a three dentate fashion. The second structure is spectroscopically described as a tetrahedral tin complex with four donating atoms (one oxygen atom coordinated to the metal and three carbon atoms belonging to the alkyl or aryl substituents), with one molecule of ligand attached. The antimicrobial activity of the tin compounds has been tested against the growth of bacteria in vitro to assess their bactericidal properties. While pentacoordinated compounds 1, 2, and 3 are described as moderate effective to noneffective drugs against both Gram-positive and Gram-negative bacteria, tetracoordinated tin(IV) compounds 4 and 5 are considered as moderate effective and most effective compounds, respectively, against the methicillin-resistant Staphylococcus aureus strains (Gram-positive). PMID:23864839

  11. Novel Organotin(IV Schiff Base Complexes with Histidine Derivatives: Synthesis, Characterization, and Biological Activity

    Directory of Open Access Journals (Sweden)

    Ariadna Garza-Ortiz

    2013-01-01

    Full Text Available Five novel tin Schiff base complexes with histidine analogues (derived from the condensation reaction between L-histidine and 3,5-di-tert-butyl-2-hydroxybenzaldehyde have been synthesized and characterized. Characterization has been completed by IR and high-resolution mass spectroscopy, 1D and 2D solution NMR (1H, 13C  and 119Sn, as well as solid state 119Sn NMR. The spectroscopic evidence shows two types of structures: a trigonal bipyramidal stereochemistry with the tin atom coordinated to five donating atoms (two oxygen atoms, one nitrogen atom, and two carbon atoms belonging to the alkyl moieties, where one molecule of ligand is coordinated in a three dentate fashion. The second structure is spectroscopically described as a tetrahedral tin complex with four donating atoms (one oxygen atom coordinated to the metal and three carbon atoms belonging to the alkyl or aryl substituents, with one molecule of ligand attached. The antimicrobial activity of the tin compounds has been tested against the growth of bacteria in vitro to assess their bactericidal properties. While pentacoordinated compounds 1, 2, and 3 are described as moderate effective to noneffective drugs against both Gram-positive and Gram-negative bacteria, tetracoordinated tin(IV compounds 4 and 5 are considered as moderate effective and most effective compounds, respectively, against the methicillin-resistant Staphylococcus aureus strains (Gram-positive.

  12. Green synthesis of novel quinoline based imidazole derivatives and evaluation of their antimicrobial activity

    Directory of Open Access Journals (Sweden)

    N.C. Desai

    2014-12-01

    Full Text Available We have described the conventional and microwave method for the synthesis of N-(4-((2-chloroquinolin-3-ylmethylene-5-oxo-2-phenyl-4,5-dihydro-1H-imidazol-1-yl(arylamides 3a–l. It is observed that the solvent-free microwave thermolysis is a convenient, rapid, high-yielding, and environmental friendly protocol for the synthesis of quinoline based imidazole derivatives when compared with conventional reaction in a solution phase. Antimicrobial activity of the newly synthesized compounds is screened in vitro on the following microbial cultures: Escherichia coli (MTCC 443, Pseudomonas aeruginosa (MTCC 1688, Staphylococcus aureus (MTCC 96, Streptococcus pyogenes (MTCC 442, Candida albicans (MTCC 227, Aspergillus niger (MTCC 282, Aspergillus clavatus (MTCC 1323. All the synthesized bio-active molecules are tested for their in vitro antimicrobial activity by bioassay namely serial broth dilution. Among these compounds 3c, 3d, 3f, 3h and 3j show significant potency against different microbial strains. All the compounds have been characterized by IR, 1H NMR, 13C NMR and mass spectral data. On the basis of statistical analysis, it is observed that these compounds give significant co-relation.

  13. Deriving hydraulic roughness from camera-based high resolution topography in field and laboratory experiments

    Science.gov (United States)

    Kaiser, Andreas; Neugirg, Fabian; Ebert, Louisa; Haas, Florian; Schmidt, Jürgen; Becht, Michael; Schindewolf, Marcus

    2016-04-01

    The hydraulic roughness, represented by Manning's n, is an essential input parameter in physically based soil erosion modeling. In order to acquire the roughness values for certain areas, on-site flow experiments have to be carried out. These results are influenced by the selection of the location of the test plot and are thereby based on the subjectiveness of the researchers. The study aims on the methodological development to acquire Manning's n by creating very high-resolution surface models with structure-from-motion approaches. Data acquisition took place during several field experiments in the Lainbach valley, southern Germany, and on agricultural sites in Saxony, eastern Germany, and in central Brazil. Rill and interrill conditions were simulated by flow experiments. In order to validate our findings stream velocity as an input for the manning equation was measured with coloured dye. Grain and aggregate sizes were derived by measuring distances from a best fit line to the reconstructed soil surface. Several diameters from D50 to D90 were tested with D90 showing best correlation between tracer experiments and photogrammetrically acquired data. A variety of roughness parameters were tested (standard deviation, random roughness, Garbrecht's n and D90). Best agreement in between the particle size and the hydraulic roughness was achieved with a non-linear sigmoid function and D90 rather than with the Garbrecht equation or statistical parameters. To consolidate these findings a laboratory setup was created to reproduce field data under controlled conditions, excluding unknown influences like infiltration and changes in surface morphology by erosion.

  14. Solution properties and emulsification properties of amino acid-based gemini surfactants derived from cysteine.

    Science.gov (United States)

    Yoshimura, Tomokazu; Sakato, Ayako; Esumi, Kunio

    2013-01-01

    Amino acid-based anionic gemini surfactants (2C(n)diCys, where n represents an alkyl chain with a length of 10, 12, or 14 carbons and "di" and "Cys" indicate adipoyl and cysteine, respectively) were synthesized using the amino acid cysteine. Biodegradability, equilibrium surface tension, and dynamic light scattering were used to characterize the properties of gemini surfactants. Additionally, the effects of alkyl chain length, number of chains, and structure on these properties were evaluated by comparing previously reported gemini surfactants derived from cystine (2C(n)Cys) and monomeric surfactants (C(n)Cys). 2C(n)diCys shows relatively higher biodegradability than does C(n)Cys and previously reported sugar-based gemini surfactants. Both critical micelle concentration (CMC) and surface tension decrease when alkyl chain length is increased from 10 to 12, while a further increase in chain length to 14 results in increased CMC and surface tension. This indicates that long-chain gemini surfactants have a decreased aggregation tendency due to the steric hindrance of the bulky spacer as well as premicelle formation at concentrations below the CMC and are poorly packed at the air/water interface. Formation of micelles (measuring 2 to 5 nm in solution) from 2C(n)diCys shows no dependence on alkyl chain length. Further, shaking the mixtures of aqueous 2C(n)diCys surfactant solutions and squalane results in the formation of oil-in-water type emulsions. The highly stable emulsions are formed using 2C₁₂diCys or 2C₁₄diCys solution and squalane in a 1:1 or 2:1 volume ratio.

  15. 奥曲肽-葡聚糖-亲和素的偶联及其与177Lu-DTPA-BIS-BIOTIN的体外结合%177Lu-DTPA-BIS-BIOTIN Binding of Octreotide-dextran-avidinated PANC-1 Cell Lines in Vitro

    Institute of Scientific and Technical Information of China (English)

    邓新荣; 杜进; 翟士桢; 沈亦佳; 罗志福

    2011-01-01

    以葡聚糖为载体,奥曲肽为导向分子合成了生长抑素配体化合物奥曲肽-葡聚糖-亲和素(Tyr3-oct-reotide-dxtran 40-avidin,TOC-Dx40-Av);在体外模拟肿瘤预定位二步法以177Lu-DTPA-BIS-BIOTIN对TOC-Dx40-Av培养的PANC-1细胞的结合特性进行了研究.将长满人源胰腺癌细胞PANC-1的24孔板置于含有TOC-Dx40-Av的缓冲液中培养,2h后洗去上清液,再用含不同浓度177Lu- DTPA-BIS-BIOTIN(177Lu-DT-PA-BIS-BIOTIN的摩尔质量范围为48.8~391 pmol)的缓冲液继续培养细胞,使177Lu-DTPA-BIS-BIOTIN 与细胞上的亲和素结合,测定细胞上结合的放射性计数,考察TOC-Dx40-Av与177 Lu-DTPA-BIS-BIOTIN的结合特性以评价合成的大分子亲和素连接物的活性.实验结果显示,奥曲肽-葡聚糖-亲和素的化学纯度>99%,其中亲和素的含量为6.46 g/L.体外细胞结合实验结果表明,177Lu-DTPA-BIS-BIOTIN能快速与细胞上连接的亲和素结合,生物素与亲和素的摩尔比约为1∶1达到平衡.%Tyr3-octreotide, dextran-40 and avidin were used to prepare octreotide-dextran-avidin (TOC-Dx40-Av). DTPA-BIS-BIOTIN was labelled with 177Lu. The in vitro soma-tostatin receptor binding study was carried out by pretargeted method using TOC-Dx40-Av and 177Lu-DTPA-BIS-BIOTIN. The 24 well cell culture plates were prepared with PANC-1 cell monolayer and then incubated with TOC-Dx40-Av. After two washed with PBS, the cells were incubated with different concentration of 177 Lu-DTPA-BIS-BIOTIN (48. 8~ 391 pmol). Cells uptake was evaluated with y counter. The results showed that the chemi- cal purity of TOC-Dx40-Av was over 99%. The results also showed that TOC-Dx40-Av remained high receptor binding affinity to somatostatin receptor which indicated that TOC-Dx40-Av could bind to 177 Lu-DTPA-BIS-BIOTIN with the molar ratio of 1 ? 1 on the cell surface.

  16. Food group diversity indicators derived from qualitative list-based questionnaire misreported some foods compared to same indicators derived from quantitative 24-hour recall in urban Burkina Faso.

    Science.gov (United States)

    Martin-Prevel, Yves; Becquey, Elodie; Arimond, Mary

    2010-11-01

    To be useful proxies of micronutrient intake at the population level in resource-limited societies, dietary diversity indicators should be simple and easy to collect in large surveys and their accuracy needs to be assessed. The present study aimed at comparing food group diversity indicators (FGI) derived from simple qualitative list-based questionnaires (qFGI) to the same indicators derived from quantitative 24-h recalls (QFGI). Both methods were administered separately on each of 3 recall days to women in 2 districts of Ouagadougou, Burkina Faso. Data were available for a total of 526 women x recalls. This study was performed within the framework of the Women's Dietary Diversity Project, which sought to analyze the relationships between various QFGI and the mean probability of adequacy (MPA) of women's diets across 11 micronutrients. The comparison between paired qFGI and QFGI scores was made both in terms of accuracy of the reporting by the list-based questionnaire, taking the QFGI as the gold standard, and in terms of performance of indicators in predicting an MPA > 60%. Examination of paired QFGI-qFGI differences revealed that the more disaggregated the FGI, the higher were the mean differences in scores. Food groups most frequently misreported often corresponded to foods put in small quantities in sauces. Overreporting by list-based questionnaires was observed for indicators applying a 15-g minimum quantity of consumption for a group to count in the score and this may result in weaker performance in predicting the MPA. These results highlight trade-offs between accuracy and simplicity when operationalizing FGI through qualitative questionnaires.

  17. Comparison of Cloud Base Height Derived from a Ground-Based Infrared Cloud Measurement and Two Ceilometers

    Directory of Open Access Journals (Sweden)

    Lei Liu

    2015-01-01

    Full Text Available The cloud base height (CBH derived from the whole-sky infrared cloud-measuring system (WSIRCMS and two ceilometers (Vaisala CL31 and CL51 from November 1, 2011, to June 12, 2012, at the Chinese Meteorological Administration (CMA Beijing Observatory Station are analysed. Significant differences can be found by comparing the measurements of different instruments. More exactly, the cloud occurrence retrieved from CL31 is 3.8% higher than that from CL51, while WSIRCMS data shows 3.6% higher than ceilometers. More than 75.5% of the two ceilometers’ differences are within ±200 m and about 89.5% within ±500 m, while only 30.7% of the differences between WSIRCMS and ceilometers are within ±500 m and about 55.2% within ±1000 m. These differences may be caused by the measurement principles and CBH retrieval algorithm. A combination of a laser ceilometer and an infrared cloud instrument is recommended to improve the capability for determining cloud occurrence and retrieving CBHs.

  18. An improved 3D MoF method based on analytical partial derivatives

    Science.gov (United States)

    Chen, Xiang; Zhang, Xiong

    2016-12-01

    MoF (Moment of Fluid) method is one of the most accurate approaches among various surface reconstruction algorithms. As other second order methods, MoF method needs to solve an implicit optimization problem to obtain the optimal approximate surface. Therefore, the partial derivatives of the objective function have to be involved during the iteration for efficiency and accuracy. However, to the best of our knowledge, the derivatives are currently estimated numerically by finite difference approximation because it is very difficult to obtain the analytical derivatives of the object function for an implicit optimization problem. Employing numerical derivatives in an iteration not only increase the computational cost, but also deteriorate the convergence rate and robustness of the iteration due to their numerical error. In this paper, the analytical first order partial derivatives of the objective function are deduced for 3D problems. The analytical derivatives can be calculated accurately, so they are incorporated into the MoF method to improve its accuracy, efficiency and robustness. Numerical studies show that by using the analytical derivatives the iterations are converged in all mixed cells with the efficiency improvement of 3 to 4 times.

  19. Degradation of carbofuran derivatives in restricted water environments: basic hydrolysis in AOT-based microemulsions.

    Science.gov (United States)

    Morales, Jorge; Manso, José A; Cid, Antonio; Lodeiro, Carlos; Mejuto, Juan Carlos

    2012-04-15

    The effect of sodium bis(2-ethylhexyl)sulfosuccinate/isooctane/water microemulsions on the stability of 2,2-dimethyl-2,3-dihydro-1-benzofuran-7-yl methylcarbamate (carbofuran, CF), 3-hydroxy-2,3-dihydro-2,2-dimethylbenzofuran-7-yl methylcarbamate (3-hydroxycarbofuran, HCF) and 3-keto-2,3-dihydro-2,2-dimethylbenzofuran-7-yl methylcarbamate (3-ketocarbofuran, KCF) in basic media has been studied. The presence of these microheterogeneous media implies a large basic hydrolysis of CF and HCF on increasing surfactant concentration and, also, on increasing water content in the microemulsion. The hydrolysis rate constants are approximately 2- and 10-fold higher than those in pure water for HCF and CF, respectively. In contrast, a steep descent in the rate of decomposition for KCF was observed. These behaviours can be ascribed to the presence of CF derivatives both in the hydrophilic phase and in the lipophilic phase, while the hydroxyl ions are only restricted to the water pool of the microemulsion (hydrophilic phase). The kinetic rate constants for the basic hydrolysis in AOT-based microemulsions have been obtained on the basis of a pseudophase model. Taking into account that an important part of soils are colloids, the possibility of the presence of restricted water environments implies that soil composition and its structure will play an important role in the stability of these carbamates. In fact, we observed that the presence of these restricted aqueous media in the environment, in particular in watersheds and in wastewaters, could reduce significantly the half-life of these pesticides (33% and 91% for HCF and CF, respectively).

  20. A Quantitative Structure-Activity Relationships (QSAR Study of Piperine Based Derivatives with Leishmanicidal Activity

    Directory of Open Access Journals (Sweden)

    Edilson Beserra Alencar Filho

    2017-04-01

    Full Text Available Leishmaniasis is a parasitic disease which represents a serious public health problem in developing countries. It is considered a neglected tropical disease, for which there is little initiative in the search for therapeutic alternatives by pharmaceutical industry. Natural products remain a great source of inspiration for obtaining bioactive molecules. In 2010, Singh and co-workers published the synthesis and in vitro biological activity of piperoyl-aminoacid conjugates, as well as of piperine, against cellular cultures of Leishmania donovani. The piperine is an alkaloid isolated from Piper nigrum that has many activities described in the literature. In this work, we present a Quantitative Structure-Activity Study of piperine derivatives tested by Singh and co-workers, aiming to highlight important molecular features for leishmanicidal activity, obtaining a mathematical model to predict the activity of new analogs. Compounds were submitted to a geometry optimization computational procedure at semiempirical level of quantum theory. Molecular descriptors for the set of compounds were calculated by E-Dragon online plataform, followed by a variable selection procedure using Ordered Predictors Selection algorithm. Validation parameters obtained showed that a good QSAR model, based on multiple linear regression, was obtained (R2 = 0.85; Q2 = 0.69, and the following conclusions regarding the structure-activity relationship were elucidated: Compounds with electronegative atoms on different substituent groups of analogs, absence of unsaturation on lateral chain, presence of ester instead of carboxyl, and large volumes (due the presence of additional aromatic rings trends to increase the activity against promastigote forms of leishmania. DOI: http://dx.doi.org/10.17807/orbital.v9i1.893

  1. Synthesis of Triazole Schiff's Base Derivatives and Their Inhibitory Kinetics on Tyrosinase Activity.

    Directory of Open Access Journals (Sweden)

    Feng Yu

    Full Text Available In the present study, new Schiff's base derivatives: (Z-4-amino-5-(2-(3- fluorobenzylidenehydrazinyl-4H-1,2,4-triazole-3-thiol (Y1, (Z-3-((2-(4-amino-5- mercapto-4H-1,2,4-triazol-3-ylhydrazonomethylphenol (Y2, (Z-2-((2-(4-amino-5- mercapto-4H-1,2,4-triazol-3-ylhydrazonomethylphenol (Y3 and 3-((Z-(2-(4- (((E-3-hydroxybenzylideneamino-5-mercapto-4H-1,2,4-triazol-3-ylhydrazonomethylphenol (Y4 were synthesized and their structures were characterized by LC-MS, IR and 1H NMR. The inhibitory effects of these compounds on tyrosinase activites were evaluated. Compounds Y1, Y2 and Y3 showed potent inhibitory effects with respective IC50 value of 12.5, 7.0 and 1.5 μM on the diphenolase activities. Moreover, the inhibition mechanisms were determined to be reversible and mixed types. Interactions of the compounds with tyrosinase were further analyzed by fluorescence quenching, copper interaction, and molecular simulation assays. The results together with the anti-tyrosinase activities data indicated that substitution on the second position of benzene ring showed superior ant-ityrosinase activities than that on third position, and that hydroxyl substitutes were better than fluorine substitutes. In addition, two benzene rings connecting to the triazole ring would produce larger steric hindrance, and affect the bonding between tyrosinase and inhibitors to decrease the inhibitory effects. The anti-tyrosinase effects of these compounds were in contrast to their antioxidant activities. In summary, this research will contribute to the development and design of antityrosinase agents.

  2. Stem cell-based delivery of brain-derived neurotrophic factor gene in the rat retina.

    Science.gov (United States)

    Park, Hae-Young Lopilly; Kim, Jie Hyun; Sun Kim, Hwa; Park, Chan Kee

    2012-08-21

    As an alternative to a viral vector, the application of stem cells to transfer specific genes is under investigation in various organs. Using this strategy may provide more effective method to supply neurotrophic factor to the neurodegenerative diseases caused by neurotrophic factor deprivation. This study investigated the possibility and efficacy of stem cell-based delivery of the brain-derived neurotrophic factor (BDNF) gene to rat retina. Rat BDNF cDNA was transduced into rat bone marrow mesenchymal stem cells (rMSCs) using a retroviral vector. Its incorporation into the experimental rat retina and the expression of BDNF after intravitreal injection or subretinal injection were detected by real-time PCR, western blot analysis, and immunohistochemical staining. For the incorporated rMSCs, retinal-specific marker staining was performed to investigate the changes in morphology and the characteristics of the stem cells. Transduction of the rMSCs by retrovirus was effective, and the transduced rMSCs expressed high levels of the BDNF gene and protein. The subretinal injection of rMSCs produced rMSC migration and incorporation into the rat retina (about 15.7% incorporation rate), and retinal BDNF mRNA and protein expression was increased at 4 weeks after transplantation. When subretinal injection of rMSCs was applied to axotomized rat retina, it significantly increased the expression of BDNF until 4 weeks after transplantation. Some of the transplanted rMSCs exhibited morphological changes, but the retinal-specific marker stain was not sufficient to indicate whether neuronal differentiation had occurred. Using mesenchymal stem cells to deliver the BDNF gene to the retina may provide new treatment for glaucoma.

  3. Curriculum-Based Measurement of Oral Reading: Evaluation of Growth Estimates Derived with Pre-Post Assessment Methods

    Science.gov (United States)

    Christ, Theodore J.; Monaghen, Barbara D.; Zopluoglu, Cengiz; Van Norman, Ethan R.

    2013-01-01

    Curriculum-based measurement of oral reading (CBM-R) is used to index the level and rate of student growth across the academic year. The method is frequently used to set student goals and monitor student progress. This study examined the diagnostic accuracy and quality of growth estimates derived from pre-post measurement using CBM-R data. A…

  4. Chiral induction in phenanthroline-derived oligoamide foldamers: an acid- and base-controllable switch in helical molecular strands.

    Science.gov (United States)

    Hu, Hai-Yu; Xiang, Jun-Feng; Yang, Yong; Chen, Chuan-Feng

    2008-03-20

    A series of phenanthroline-derived oligoamides bearing a chiral (R)-phenethylamino end group were synthesized that displayed chiral helical induction and subsequently formed one-hand helical foldamers in solution. Moreover, an acid- and base-controllable switch in the helical molecular strands was observed, which has been demonstrated by NMR, UV-vis, and circular dichroism spectroscopy.

  5. Imaging and Intracellular Tracking of Cancer-Derived Exosomes Using Single-Molecule Localization-Based Super-Resolution Microscope.

    Science.gov (United States)

    Chen, Chen; Zong, Shenfei; Wang, Zhuyuan; Lu, Ju; Zhu, Dan; Zhang, Yizhi; Cui, Yiping

    2016-10-05

    Exosomes are small membrane vesicles secreted by cells and enriched with plenty of proteins. Considering their significant roles in different physical activities and potential value for diagnostic drug delivery, researchers have put great efforts in in vitro tracking and content analysis of exosomes. Recently, the emergence of different kinds of super-resolution microscopy provides powerful tools for exosome study. Here, we demonstrate the application of single-molecule localization based super-resolution imaging technique in the imaging and tracking of cancer-derived exosomes. In the experiment, first, cancer-derived exosomes are extracted from the culture media of tumor cells. Then the exosome membrane receptors are labeled with photoswitchable probes, which allow super-resolution imaging of these membrane receptors via photoactivated localization microscopy (PALM) or stochastic optical reconstruction microscopy (STORM). By using human breast cancer cell-derived exosomes, we demonstrated simultaneous dual-color PALM/STORM imaging of two kinds of membrane receptors on the exosome membrane. Moreover, the successful labeling and imaging of exosomes make it possible to observe the interaction between cancer-derived exosomes and normal cells. Meanwhile, we realized the colocalization of cancer-derived exosomes and lysosomes in recipient cells with PALM/STORM imaging. Since exosomes play a vital role in intercellular communications, we anticipate that the presented PALM/STORM-based imaging and tracking of exosomes holds a great potential in the investigation of the mechanism of exosome-mediated cancer metastasis.

  6. Synthesis, Characterization and Biological Activity of Transition Metals with Schiff Base Derived from Adamantaneamine and o-Vanillin

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    Five new solid complexes were synthesized about transition metals with Schiff base(L,C18H23NO2) derived from adamantaneamine and o-vanillin, and characterized by elemental analysis, molar conductance, infrared spectra, UV-vis spectra, thermal analysis. Their chemical formula are [ML2](ClO4)2 (M= Mn,Co,Ni,Cu,Zn),and the coordination numbers are four. The antibacterial activity of Schiff base ligand and its complexes was studied.

  7. Impact of liver cirrhosis on liver enhancement at Gd-EOB-DTPA enhanced MRI at 3 Tesla

    Energy Technology Data Exchange (ETDEWEB)

    Verloh, N., E-mail: niklas.verloh@stud.uni-regensburg.de [Department of Radiology, University Hospital Regensburg, Regensburg (Germany); Haimerl, M.; Rennert, J.; Müller-Wille, R.; Nießen, C. [Department of Radiology, University Hospital Regensburg, Regensburg (Germany); Kirchner, G. [Department of Gastroenterology, University Hospital Regensburg, Regensburg (Germany); Scherer, M.N. [Department of Surgery, University Hospital Regensburg, Regensburg (Germany); Schreyer, A.G.; Stroszczynski, C.; Fellner, C.; Wiggermann, P. [Department of Radiology, University Hospital Regensburg, Regensburg (Germany)

    2013-10-01

    Purpose: The purpose of this study was to assess differences in enhancement effects of liver parenchyma between normal and cirrhotic livers on dynamic, Gd-EOB-DTPA enhanced MRI at 3 T. Materials and methods: 93 patients with normal (n = 54) and cirrhotic liver (n = 39; Child–Pugh class A, n = 18; B, n = 16; C, n = 5) underwent contrast-enhanced MRI with liver specific contrast media at 3 T. T1-weighted volume interpolated breath hold examination (VIBE) sequences with fat suppression were acquired before contrast injection, in the arterial phase (AP), in the late arterial phase (LAP), in the portal venous phase (PVP), and in the hepatobiliary phase (HBP) after 20 min. The relative enhancement (RE) of the signal intensity of the liver parenchyma was calculated for all phases. Results: Mean RE was significantly different among all evaluated groups in the hepatobiliary phase and with increasing severity of liver cirrhosis, a decreasing, but still significant reduction of RE could be shown. Phase depending changes of RE for each group were observed. In case of non-cirrhotic liver or Child–Pugh Score A cirrhosis mean RE showed a significant increase between AP, LAP, PVP and HBP. For Child–Pugh B + C cirrhosis RE increased until PVP, however, there was no change in case of B cirrhosis (p = 0.501) and significantly reduced in case of C cirrhosis (p = 0.043) during HBP. Conclusion: RE of liver parenchyma is negatively affected by increased severity of liver cirrhosis, therefore diagnostic value of HBP could be limited in case of Child Pugh B + C cirrhosis.

  8. 信息动态%Preparation of Polysaccharide Derivatives-based Composite Chiral Stationary Phases and Their Chiral Recognition

    Institute of Scientific and Technical Information of China (English)

    2011-01-01

    Two coated-type composite chiral stationary phases (CSPs) were prepared based on cellulose tris(3,5-dimethylphenylcarbamate) (CDMPC) and amylose tris (3,5-dimethylphenylcarbamate)(ADMPC) by coating the corresponding derivatives onto 3-aminopropyl silica gel separately and then mixing or by coating the mixed derivatives onto silica gel. The CSPs containing only CDMPC or ADMPC were also prepared for comparison. The mixing method does not significantly influence the enantioselectivities. The composite CSPs generally show chiral recognition abilities intermediate between those of the two individual phases, while some racemates were poorer and at the same time one was better resolved on the composite CSPs.

  9. Monte Carlo method based QSAR modeling of maleimide derivatives as glycogen synthase kinase-3β inhibitors.

    Science.gov (United States)

    Živković, Jelena V; Trutić, Nataša V; Veselinović, Jovana B; Nikolić, Goran M; Veselinović, Aleksandar M

    2015-09-01

    The Monte Carlo method was used for QSAR modeling of maleimide derivatives as glycogen synthase kinase-3β inhibitors. The first QSAR model was developed for a series of 74 3-anilino-4-arylmaleimide derivatives. The second QSAR model was developed for a series of 177 maleimide derivatives. QSAR models were calculated with the representation of the molecular structure by the simplified molecular input-line entry system. Two splits have been examined: one split into the training and test set for the first QSAR model, and one split into the training, test and validation set for the second. The statistical quality of the developed model is very good. The calculated model for 3-anilino-4-arylmaleimide derivatives had following statistical parameters: r(2)=0.8617 for the training set; r(2)=0.8659, and r(m)(2)=0.7361 for the test set. The calculated model for maleimide derivatives had following statistical parameters: r(2)=0.9435, for the training, r(2)=0.9262 and r(m)(2)=0.8199 for the test and r(2)=0.8418, r(av)(m)(2)=0.7469 and ∆r(m)(2)=0.1476 for the validation set. Structural indicators considered as molecular fragments responsible for the increase and decrease in the inhibition activity have been defined. The computer-aided design of new potential glycogen synthase kinase-3β inhibitors has been presented by using defined structural alerts.

  10. Spectroscopic and biological activities studies of bivalent transition metal complexes of Schiff bases derived from condensation of 1,4-phenylenediamine and benzopyrone derivatives.

    Science.gov (United States)

    Sherif, Omaima E; Abdel-Kader, Nora S

    2014-01-03

    Many tools of analysis such as elemental analyses, infrared, ultraviolet-visible, electron spin resonance (ESR) and thermal analysis, as well as conductivity and magnetic susceptibility measurements were used to elucidate the structures of the newly prepared Co(II), Ni(II) and Cu(II) complexes with Schiff bases derived from the condensation of 1,4-phenylenediamine with 6-formyl-7-hydroxy-5-methoxy-2-methylbenzo-pyran-4-one (H2L) or 5,7-dihydroxy-6-formyl-2-methylbenzopyran-4-one (H4L). The data showed that all formed complexes are 1:1 or 2:2 (M:L) and non-electrolyte chelates. The Co(II) and Cu(II) complexes of the two Schiff bases were screened for antibacterial activities by the disk diffusion method. The antibacterial activity was screened using Escherichia coli and Staphylococcus capitis but the antifungal activity was examined by using Aspergillus flavus and Candida albicans. The Results showed that the tested complexes have antibacterial, except CuH4L, but not antifungal activities.

  11. Lunar textural analysis based on WAC-derived kilometer-scale roughness and entropy maps

    Science.gov (United States)

    Li, Bo; Wang, XueQiang; Zhang, Jiang; Chen, Jian; Ling, Zongcheng

    2016-06-01

    In general, textures are thought to be some complicated repeated patterns formed by elements, or primitives which are sorted in certain rules. Lunar surfaces record the interactions between its outside environment and itself, thus, based on high-resolution DEM model or image data, there are some topographic features which have different roughness and entropy values or signatures on lunar surfaces. Textures of lunar surfaces can help us to concentrate on typical topographic and photometric variations and reveal the relationships between obvious features (craters, impact basins, sinuous rilles (SRs) and ridges) with resurfacing processes on the Moon. In this paper, the term surface roughness is an expression of the variability of a topographic or photometric surface at kilometer scale, and the term entropy can characterize the variability inherent in a geological and topographic unit and evaluate the uncertainty of predictions made by a given geological process. We use the statistical moments of gray-level histograms in different-sized neighborhoods (e.g., 3, 5, 10, 20, 40 and 80 pixels) to compute the kilometer-scale roughness and entropy values, using the mosaic image from 70°N to 70°S obtained by Lunar Reconnaissance Orbiter (LRO) Wide Angle Camera (WAC). Large roughness and entropy signatures were only found in the larger scale maps, while the smallest 3-pixel scale map had more disorderly and unsystematic textures. According to the entropy values in 10-pixel scale entropy map, we made a frequency curve and categorized lunar surfaces into three types, shadow effects, maria and highlands. A 2D scatter plot of entropy versus roughness values was produced and we found that there were two point clusters corresponding to the highlands and maria, respectively. In the last, we compared the topographic and photometric signatures derived from Lunar Orbiter Laser Altimeter (LOLA) data and WAC mosaic image. On the lunar surfaces, the ridges have obvious multilevel

  12. Diester Derivatives from Chemically Modiifed Waste Cooking Oil as Substitute for Petroleum Based Lubricating Oils

    Institute of Scientific and Technical Information of China (English)

    Xiang Shuo; Chen Ligong; Xu Lan; Li Liang; Yang Xin; Zhu Liye

    2015-01-01

    In order to provide a new way for waste cooking oil (WCO) resource utilization, several diester derivatives were obtained from WCO through a three-step chemical modifications, viz.:transesterification, epoxidation and oxirane ring opening with carboxylic acids. The effects of the chain length of side chain groups on the viscosity, acid value, low temper-ature lfuidity, thermo-oxidative stability, tribological properties and surface tension of diester derivatives were investigated. The results showed that increasing the chain length of side chain groups had a positive inlfuence on the viscosity, viscosity index, acid value, pour point, friction coefifcient and wear scar diameter along with a negative inlfuence on the oxidation onset temperature, volatile loss, insoluble deposit, maximum non-seizure load and surface tension. These diester derivatives exhibited improved physicochemical and tribological properties that make themselves promising environmentally friendly biolubricant basestocks.

  13. Detection and classification of different liver lesions: Comparison of Gd-EOB-DTPA-enhanced MRI versus multiphasic spiral CT in a clinical single centre investigation

    Energy Technology Data Exchange (ETDEWEB)

    Böttcher, Joachim [Institute of Diagnostic and Interventional Radiology, SRH Clinic Gera, Str. des Friedens 122, 07548 Gera (Germany); Hansch, Andreas [Institute of Diagnostic and Interventional Radiology, Friedrich-Schiller-University, Jena University Hospital, Erlanger Allee 101, 07740 Jena (Germany); Pfeil, Alexander [Department of Internal Medicine III, Friedrich-Schiller-University, Jena University Hospital, Erlanger Allee 101, 07740 Jena (Germany); Schmidt, Peter [Institute of Diagnostic and Interventional Radiology, Friedrich-Schiller-University, Jena University Hospital, Erlanger Allee 101, 07740 Jena (Germany); Malich, Ansgar [Institute of Diagnostic Radiology, Suedharz Clinic Nordhausen, Dr. Robert-Koch-Str. 39, 99734 Nordhausen (Germany); Schneeweiss, Albrecht [Institute of Diagnostic and Interventional Radiology, Friedrich-Schiller-University, Jena University Hospital, Erlanger Allee 101, 07740 Jena (Germany); Maurer, Martin H.; Streitparth, Florian [Department of Radiology, Charité University Medicine Berlin, Campus Virchow Clinic, Augustenburger Platz 1, 13353 Berlin (Germany); Teichgräber, Ulf K. [Institute of Diagnostic and Interventional Radiology, Friedrich-Schiller-University, Jena University Hospital, Erlanger Allee 101, 07740 Jena (Germany); Renz, Diane M., E-mail: diane.renz@charite.de [Department of Radiology, Charité University Medicine Berlin, Campus Virchow Clinic, Augustenburger Platz 1, 13353 Berlin (Germany)

    2013-11-01

    Objective: To compare the diagnostic efficacy of Gd-EOB-DTPA-enhanced magnetic resonance imaging (MRI) vs. multidetector computed tomography (MDCT) for the detection and classification of focal liver lesions, differentiated also for lesion entity and size; a separate analysis of pre- and postcontrast images as well as T2-weighted MRI sequences of focal and exclusively solid lesions was integrated. Methods: Twenty-nine patients with 130 focal liver lesions underwent MDCT (64-detector-row; contrast medium iopromide; native, arterial, portalvenous, venous phase) and MRI (1.5-T; dynamic and tissue-specific phase 20 min after application of Gd-EOB-DTPA). Hepatic lesions were verified against a standard of reference (SOR). CT and MR images were independently analysed by four blinded radiologists on an ordinal 6-point-scale, determining lesion classification and diagnostic confidence. Results: Among 130 lesions, 68 were classified as malignant and 62 as benign by SOR. The detection of malignant and benign lesions differed significantly between combined and postcontrast MRI vs. MDCT; overall detection rate was 91.5% for combined MRI and 80.4% for combined MDCT (p < 0.05). Considering all four readers together, combined MDCT achieved sensitivity of 66.2%, specificity of 79.0%, and diagnostic accuracy of 72.3%; combined MRI reached superior diagnostic efficacy: sensitivity 86.8%, specificity 94.4%, accuracy 90.4% (p < 0.05). Differentiated for lesion size, in particular lesions <20 mm revealed diagnostic benefit by MRI. Postcontrast MRI also achieved higher overall sensitivity, specificity, and accuracy compared to postcontrast MDCT for focal and exclusively solid liver lesions (p < 0.05). Conclusion: Combined and postcontrast Gd-EOB-DTPA-enhanced MRI provided significantly higher overall detection rate and diagnostic accuracy, including low inter-observer variability, compared to MDCT in a single centre study.

  14. MRI for characterization of primary tumors in the non-cirrhotic liver: Added value of Gd-EOB-DTPA enhanced hepatospecific phase

    Energy Technology Data Exchange (ETDEWEB)

    Donati, Olivio F.; Hunziker, Roger; Fischer, Michael A. [Institute of Diagnostic and Interventional Radiology, University Hospital Zurich, Zurich (Switzerland); Raptis, Dimitri A.; Breitenstein, Stefan [Department of Visceral and Transplant Surgery, Swiss Hepato-Pancreato-Biliary (HPB) Center, University Hospital Zurich, Zurich (Switzerland); Patak, Michael A., E-mail: Michael.Patak@hirslanden.ch [Institute of Diagnostic and Interventional Radiology, University Hospital Zurich, Zurich (Switzerland); Clinic Hirslanden, Hirslanden Hospital Group, Zurich (Switzerland)

    2014-07-15

    Purpose: To evaluate the added value of hepatospecific phase in Gd-EOB-DTPA enhanced magnetic resonance imaging (MRI) in patients with primary tumors in non-cirrhotic liver. Methods: Twenty-nine patients (median, 39 years; range, 18–81 years; 11 male) underwent preoperative Gd-EOB-DTPA enhanced MRI including hepatospecific phase after 10 and 20 min of contrast injection at four institutions in Europe, North America and New Zealand. Images were evaluated by three different readers (R1–R3) who characterized liver tumors with and without consultation of the hepatospecific phase images. Confidence in diagnosis was scored on a visual analog scale from 1 to 10. Histopathology (adenoma, n = 5; focal nodular hyperplasia, n = 11 and hepatocellular carcinoma, n = 13) in all patients served as the standard of reference. Differences were evaluated using the McNemar and Wilcoxon signed rank test. Results: Without hepatospecific phase images available, 22 (76%), 19 (66%) and 19 (66%) of 29 tumors were characterized correctly by the three readers respectively. Mean confidence in diagnosis was 6.1, 5.7 and 5.8. With the hepatospecific phase included, characterization of liver tumors did not change significantly with 21 (72%), 23 (79%) and 19 (66%) of 29 tumors diagnosed correctly (p > 0.05). According confidence ratings increased to 6.3, 6.5 and 7.7, respectively. Increase in diagnostic confidence was significant for R2 and R3 (p < 0.05) and independent of reader's experience. Conclusion: The additional hepatospecific phase in Gd-EOB-DTPA enhanced MRI did not significantly increase diagnostic accuracy in characterization of primary tumors in the non-cirrhotic liver. However, 2/3 readers showed a significant increase in diagnostic confidence after consultation of the hepatospecific phase.

  15. Comparison of different magnetic resonance cholangiography techniques in living liver donors including Gd-EOB-DTPA enhanced T1-weighted sequences.

    Directory of Open Access Journals (Sweden)

    Sonja Kinner

    Full Text Available Preoperative evaluation of potential living liver donors (PLLDs includes the assessment of the biliary anatomy to avoid postoperative complications. Aim of this study was to compare T2-weighted (T2w and Gd-EOB-DTPA enhanced T1-weighted (T1w magnetic resonance cholangiography (MRC techniques in the evaluation of PLLDs.30 PLLDs underwent MRC on a 1.5 T Magnetom Avanto (Siemens, Erlangen, Germany using (A 2D T2w HASTE (Half Fourier Acquisition Single Shot Turbo Spin Echo fat saturated (fs in axial plane, (B 2D T2w HASTE fs thick slices in coronal plane, (C free breathing 3D T2w TSE (turbo spin echo RESTORE (high-resolution navigator corrected plus (D maximum intensity projections (MIPs, (E T2w SPACE (sampling perfection with application optimized contrasts using different flip angle evolutions plus (F MIPs and (G T2w TSE BLADE as well as Gd-EOB-DTPA T1w images without (G and with (H inversion recovery. Contrast enhanced CT cholangiography served as reference imaging modality. Two independent reviewers evaluated the biliary tract anatomy on a 5-point scale subjectively and objectively. Data sets were compared using a Mann-Whitney-U-test. Kappa values were also calculated.Source images and maximum intensity projections of 3D T2w TSE sequences (RESTORE and SPACE proved to be best for subjective and objective evaluation directly followed by 2D HASTE sequences. Interobserver variabilities were good to excellent (k = 0.622-0.804.3D T2w sequences are essential for preoperative biliary tract evaluation in potential living liver donors. Furthermore, our results underline the value of different MRCP sequence types for the evaluation of the biliary anatomy in PLLDs including Gd-EOB-DTPA enhanced T1w MRC.

  16. Synthesis and Chromatographic Properties of New β-Cyclodextrin Derivatives with α-Schiff Base Groups for HPLC

    Institute of Scientific and Technical Information of China (English)

    Min FANG; Zhi Ming ZHOU; Ai Qin LUO

    2005-01-01

    β-Cyclodextrin 1 was directly oxidized to the corresponding monoaldehyde 2 on their primary faces by cyclized 2-iodoxybenzoic acid(IBX) in DMSO, followed by the synthesis of β-cyclodextrin derivatives bearing Schiff-base group 3. A new chiral stationary phase(BCDS 6)was then prepared by immobilization of β-cyclodextrin derivative with α-Schiff base group onto the surface of sillica gel. A series of compounds with amino groups were readily separated using this CSP. Methanol and acetonitrile were tested as the mobile phase while the influence of temperature and the addition of aqueous triethylammonium acetate buffer to the mobile phase was also innvestigated. Ferrocene ligand with Schiff-based groups have been separated satisfactorilly on BCDS column.

  17. Synthesis and NMR study of N-heterocyclic carbenes (NHC precursors derived from Tröger’s Base

    Directory of Open Access Journals (Sweden)

    Eric MUSENGIMANA

    2014-01-01

    Full Text Available Since the discovery of olefin metathesis reactions, various catalysts have been developed but those based on Ruthenium and N-heterocyclic carbenes (NHC ligands are particularly more efficient. Herein, we report a synthesis of NHC precursors derived from the Tröger’s base and its analogues which can be used in the synthesis of the new kind of metathesis catalysts. The NHC derived from the Tröger’s base present a particular chirality that could affect stereoselectivity of catalysis, and the form “V” of these ligands implies that the aromatic substituents point totally outside the coordination sphere of the metal, where would be no steric hindrance unlike what happens with the usual NHC.

  18. Novel self-associative and multiphase nanostructured soft carriers based on amphiphilic hyaluronic acid derivatives

    DEFF Research Database (Denmark)

    Eenschooten, Corinne; Vaccaro, Andrea; Delie, Florence

    2012-01-01

    The purpose of the present study was to investigate the physicochemical properties in aqueous media of amphiphilic hyaluronic acid (HA) derivatives obtained by reaction of HA’s hydroxyl groups with octenyl succinic anhydride (OSA). The self-associative properties of the resulting octenyl succinic...... anhydridemodified hyaluronic acid (OSA-HA) derivatives were studied by fluorescence spectroscopy using Nile Red as fluorophore. The morphology, size and surface charge of the OSA-HA assemblies were determined by transmission electron microscopy, dynamic light scattering and by measuring their electrophoretic...

  19. Relationship between Aldose reductase and superoxide dismutase inhibition capacities of indole-based analogs of melatonin derivatives

    Directory of Open Access Journals (Sweden)

    Daş-Evcimen N.

    2009-01-01

    Full Text Available Aldose reductase (AR has been implicated in the etiology of diabetic complications. Under diabetic conditions, the elevated vascular glucose level causes an increased flux through the polyol pathway, which induces functional and morphological changes associated with secondary diabetic complications such as cataract, neuropathy, and nephrop­athy. Oxidative stress, antioxidants, and the polyol pathway have recently been found to be linked in pathological states. A large number of structurally different compounds have been studied as potent in vitro AR inhibitors (ARIs. However, with few exceptions, these compounds did not show clinical benefit, and some even produced serious side effects. In view of the ARI activity of certain indole derivative compounds and antioxidant properties of melatonin, we investigated some indole-based analogs of melatonin derivatives. Antioxidant and ARI activity tests were applied to nine indole derivatives that are substituted at the third and fifth positions. Also, the relationship between ARI and antioxidant enzyme activity is discussed.

  20. Numerical simulation of aerodynamic derivatives and critical wind speed for long-span bridges based on simplified steady wind field

    Science.gov (United States)

    Xin, Dabo; Ou, Jinping

    2007-06-01

    Combining the computational fluid dynamics-based numerical simulation with the forced vibration technique for extraction of aerodynamic derivatives, an approach for calculating the aerodynamic derivatives and the critical flutter wind speed for long-span bridges is presented in this paper. The RNG k-ɛ turbulent model is introduced to establish the governing equations, including the continuity equation and the Navier-Stokes equations, for solving the wind flow field around a two-dimensional bridge section. To illustrate the effectiveness and accuracy of the proposed approach, a simple application to the Hume Bridge in China is provided, and the numerical results show that the aerodynamic derivatives and the critical flutter wind speed obtained agree well with the wind tunnel test results.

  1. Numerical simulation of aerodynamic derivatives and critical wind speed for long-span bridges based on simplified steady wind field

    Institute of Scientific and Technical Information of China (English)

    Xin Dabo; Ou Jinping

    2007-01-01

    Combining the computational fluid dynamics-based numerical simulation with the forced vibration technique for extraction of aerodynamic derivatives, an approach for calculating the aerodynamic derivatives and the critical flutter wind speed for long-span bridges is presented in this paper. The RNG κ-ε turbulent model is introduced to establish the governing equations, including the continuity equation and the Navier-Stokes equations, for solving the wind flow field around a two-dimensional bridge section. To illustrate the effectiveness and accuracy of the proposed approach, a simple application to the Hume Bridge in China is provided, and the numerical results show that the aerodynamic derivatives and the critical flutter wind speed obtained agree well with the wind tunnel test results.

  2. Importance of timing of post-contrast MRI in rheumatoid arthritis: what happens during the first 60 minutes after IV gadolinium-DTPA?

    DEFF Research Database (Denmark)

    Østergaard, Mikkel; Klarlund, Mette

    2001-01-01

    compromises the differentiation of synovium from joint fluid. OBJECTIVE: To determine the time period after IV MRI contrast (gadolinium-DTPA (Gd)) injection in which synovial membrane volume determination is reliable. METHODS: MRI of five RA knees with clinical synovitis was carried out, with axial, T(1...... threshold. Thereafter, the measured volumes remained practically unchanged. CONCLUSION: This study suggests that MR image acquisition in arthritic knee joints should be performed within the initial approximately 10 minutes after gadolinium contrast injection to achieve the most accurate distinction between...

  3. Evaluation of the absorbed dose to the kidneys due to Tc{sup 99m} (DTPA) / Tc{sup 99m} (Mag3) and Tc{sup 99m} (Dmsa); Evaluacion de la dosis absorbida en los rinones debido al Tc{sup 99m} (DTPA) / Tc{sup 99m} (MAG3) y Tc{sup 99m} (DMSA)

    Energy Technology Data Exchange (ETDEWEB)

    Vasquez A, M.; Murillo C, F.; Castillo D, C.; Rocha J, J.; Sifuentes D, Y.; Sanchez S, P. [Universidad Nacional de Trujillo, Av. Juan Pablo II s/n, Trujillo (Peru); Idrogo C, J.; Marquez P, F., E-mail: marvva@hotmail.com [Instituto Nacional de Enfermedades Neoplasicas, Av. Angamos 2520, Lima (Peru)

    2015-10-15

    The absorbed dose in the kidneys of adult patients has been assessed using the biokinetics of radiopharmaceuticals containing Tc{sup 99m} (DTPA) / Tc{sup 99m} (Mag3) or Tc{sup 99m} (Dmsa).The absorbed dose was calculated using the formalism MIRD and the Cristy-Eckerman representation for the kidneys. The absorbed dose to the kidneys due to Tc{sup 99m} (DTPA) / Tc{sup 99m} (Mag3), are given by 0.00466 mGy.MBq{sup -1} / 0.00339 mGy.MBq{sup -1}. Approximately 21.2% of the absorbed dose is due to the bladder (content) and the remaining tissue, included in biokinetics of Tc{sup 99m} (DTPA) / Tc{sup 99m} (Mag3). The absorbed dose to the kidneys due to Tc{sup 99m} (Dmsa) is 0.17881 mGy.MBq{sup -1}. Here, 1.7% of the absorbed dose is due to the bladder, spleen, liver and the remaining tissue, included in biokinetics of Tc{sup 99m} (Dmsa). (Author)

  4. Derivation of site-specific relationships between hydraulic parameters and p-wave velocities based on hydraulic and seismic tomography

    Energy Technology Data Exchange (ETDEWEB)

    Brauchler, R.; Doetsch, J.; Dietrich, P.; Sauter, M.

    2012-01-10

    In this study, hydraulic and seismic tomographic measurements were used to derive a site-specific relationship between the geophysical parameter p-wave velocity and the hydraulic parameters, diffusivity and specific storage. Our field study includes diffusivity tomograms derived from hydraulic travel time tomography, specific storage tomograms, derived from hydraulic attenuation tomography, and p-wave velocity tomograms, derived from seismic tomography. The tomographic inversion was performed in all three cases with the SIRT (Simultaneous Iterative Reconstruction Technique) algorithm, using a ray tracing technique with curved trajectories. The experimental set-up was designed such that the p-wave velocity tomogram overlaps the hydraulic tomograms by half. The experiments were performed at a wellcharacterized sand and gravel aquifer, located in the Leine River valley near Göttingen, Germany. Access to the shallow subsurface was provided by direct-push technology. The high spatial resolution of hydraulic and seismic tomography was exploited to derive representative site-specific relationships between the hydraulic and geophysical parameters, based on the area where geophysical and hydraulic tests were performed. The transformation of the p-wave velocities into hydraulic properties was undertaken using a k-means cluster analysis. Results demonstrate that the combination of hydraulic and geophysical tomographic data is a promising approach to improve hydrogeophysical site characterization.

  5. Real-Time Algebraic Derivative Estimations Using a Novel Low-Cost Architecture Based on Reconfigurable Logic

    Directory of Open Access Journals (Sweden)

    Rafael Morales

    2014-05-01

    Full Text Available Time derivative estimation of signals plays a very important role in several fields, such as signal processing and control engineering, just to name a few of them. For that purpose, a non-asymptotic algebraic procedure for the approximate estimation of the system states is used in this work. The method is based on results from differential algebra and furnishes some general formulae for the time derivatives of a measurable signal in which two algebraic derivative estimators run simultaneously, but in an overlapping fashion. The algebraic derivative algorithm presented in this paper is computed online and in real-time, offering high robustness properties with regard to corrupting noises, versatility and ease of implementation. Besides, in this work, we introduce a novel architecture to accelerate this algebraic derivative estimator using reconfigurable logic. The core of the algorithm is implemented in an FPGA, improving the speed of the system and achieving real-time performance. Finally, this work proposes a low-cost platform for the integration of hardware in the loop in MATLAB.

  6. A derivative based simplified phase tracker for a single fringe pattern demodulation

    Science.gov (United States)

    Deepan, B.; Quan, C.; Tay, C. J.

    2016-08-01

    In this paper, a novel fringe demodulation method for the estimation of phase and its first-order derivative from a closed-fringe interferogram is proposed. The proposed method determines the phase derivatives in both x&y directions from fringe orientation and density. The phase derivatives are subsequently used to determine phase values using a novel simplified phase tracker. In the phase tracking model, the complexity of the cost function is reduced using predetermined derivatives so computation time required for phase tracking is reduced considerably. The proposed model is more robust while dealing with saddle points in fringes than the conventional phase tracker model. Hence it does not require any specialized scanning strategy. The proposed method is validated with simulated and experimental fringe patterns (obtained using electronic speckle pattern interferometry and optical holographic interferometry) and a comparison study is carried out with conventional regularized phase tracker. The simulation results show that the proposed method has good accuracy and requires less computation time than existing phase-tracking algorithms. The experimental results demonstrate the robustness of the proposed method against speckle noise and its practical applicability for static and dynamic applications.

  7. Synthesis and Characterization of Novel Mercapto-end Ferrocenyl Derivatives with Schiff Base Linkage

    Institute of Scientific and Technical Information of China (English)

    Hai Feng SONG; Miao CHEN; Yong Min LIANG; Ling SHI; Guo Sheng HUANG

    2004-01-01

    Novel ferrocenyl derivatives of p-(10-mercaptodecyloxy) benzal 4-ferrocenylaniline have been prepared and characterized by element analysis, 1HNMR, and FAB-MS. Cyclic voltammetry has been used to characterize the self-assembled monolayers formed from the compounds on the gold electrodes.

  8. On the Mass Fractal Character of Si-Based Structural Networks in Amorphous Polymer Derived Ceramics

    Science.gov (United States)

    Sen, Sabyasachi; Widgeon, Scarlett

    2015-01-01

    The intermediate-range packing of SiNxC4−x (0 ≤ x ≤ 4) tetrahedra in polysilycarbodiimide and polysilazane-derived amorphous SiCN ceramics is investigated using 29Si spin-lattice relaxation nuclear magnetic resonance (SLR NMR) spectroscopy. The SiCN network in the polysilylcarbodiimide-derived ceramic consists predominantly of SiN4 tetrahedra that are characterized by a 3-dimensional spatial distribution signifying compact packing of such units to form amorphous Si3N4 clusters. On the other hand, the SiCN network of the polysilazane-derived ceramic is characterized by mixed bonded SiNxC4−x tetrahedra that are inefficiently packed with a mass fractal dimension of Df ~2.5 that is significantly lower than the embedding Euclidean dimension (D = 3). This result unequivocally confirms the hypothesis that the presence of dissimilar atoms, namely, 4-coordinated C and 3-coordinated N, in the nearest neighbor environment of Si along with some exclusion in connectivity between SiCxN4−x tetrahedra with widely different N:C ratios and the absence of bonding between C and N result in steric hindrance to an efficient packing of these structural units. It is noted that similar inefficiencies in packing are observed in polymer-derived amorphous SiOC ceramics as well as in proteins and binary hard sphere systems.

  9. On the Mass Fractal Character of Si-Based Structural Networks in Amorphous Polymer Derived Ceramics

    Directory of Open Access Journals (Sweden)

    Sabyasachi Sen

    2015-03-01

    Full Text Available The intermediate-range packing of SiNxC4−x (0 ≤ x ≤ 4 tetrahedra in polysilycarbodiimide and polysilazane-derived amorphous SiCN ceramics is investigated using 29Si spin-lattice relaxation nuclear magnetic resonance (SLR NMR spectroscopy. The SiCN network in the polysilylcarbodiimide-derived ceramic consists predominantly of SiN4 tetrahedra that are characterized by a 3-dimensional spatial distribution signifying compact packing of such units to form amorphous Si3N4 clusters. On the other hand, the SiCN network of the polysilazane-derived ceramic is characterized by mixed bonded SiNxC4−x tetrahedra that are inefficiently packed with a mass fractal dimension of Df ~2.5 that is significantly lower than the embedding Euclidean dimension (D = 3. This result unequivocally confirms the hypothesis that the presence of dissimilar atoms, namely, 4-coordinated C and 3-coordinated N, in the nearest neighbor environment of Si along with some exclusion in connectivity between SiCxN4−x tetrahedra with widely different N:C ratios and the absence of bonding between C and N result in steric hindrance to an efficient packing of these structural units. It is noted that similar inefficiencies in packing are observed in polymer-derived amorphous SiOC ceramics as well as in proteins and binary hard sphere systems.

  10. A Detailed Derivation of Gaussian Orbital-Based Matrix Elements in Electron Structure Calculations

    Science.gov (United States)

    Petersson, T.; Hellsing, B.

    2010-01-01

    A detailed derivation of analytic solutions is presented for overlap, kinetic, nuclear attraction and electron repulsion integrals involving Cartesian Gaussian-type orbitals. It is demonstrated how s-type orbitals can be used to evaluate integrals with higher angular momentum via the properties of Hermite polynomials and differentiation with…

  11. Boundary Value Technique for Initial Value Problems Based on Adams-Type Second Derivative Methods

    Science.gov (United States)

    Jator, S. N.; Sahi, R. K.

    2010-01-01

    In this article, we propose a family of second derivative Adams-type methods (SDAMs) of order up to 2k + 2 ("k" is the step number) for initial value problems. The methods are constructed through a continuous approximation of the SDAM which is obtained by multistep collocation. The continuous approximation is used to obtain initial value methods,…

  12. Development of a Cell-based Model to Derive Direct Runoff Hydrographs for Ungauged Mountainous Basins

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    A model to derive direct runoff hydrograph for an ungauged basin using the physical properties of the basin is presented. The basin is divided into grid cells and canal elements. Overland flow is generated from each grid cell of the basin by application of continuous effective rainfall of 1 mm/hr to the basin. The flow generated is routed through downstream grid cells and the canal elements using the kinematic wave approach. The travel time for direct runoff from each grid cell to the basin outlet is calculated and the S-curve is derived for the basin.The S-curve is used to derive the unit hydrograph of a given duration for the basin. The model, referred as Cell-basin model was applied to the Upper Kotmale Basin in Sri Lanka and the model predictions of direct runoff hydrographs for rainfall events agreed with the observations to a reasonable accuracy. Comparison of the unit hydrographs obtained from the model and from the conventional Snyder's synthetic unit hydrograph using regionalized parameters assuming the basin as an ungauged basin, with the unit hydrograph derived from the observations showed that the model predicted unit hydrograph was more suitable than that obtained by Snyder's method for Sri Lankan up country basins. Thus, the present model is a useful tool to obtain direct runoff hydrograph for ungauged basins.

  13. Highly efficient non-doped blue organic light emitting devices based on anthracene–pyridine derivatives

    Energy Technology Data Exchange (ETDEWEB)

    Haykir, Gulcin; Tekin, Emine; Atalar, Taner; Türksoy, Figen

    2013-12-02

    Four different 2-(10-aryl)anthracen-9-yl)pyridine derivatives 5a–d were synthesized via the Suzuki cross-coupling reaction. Photo-physical characteristics of these materials having strong electron donating or electron withdrawing groups were explored. Multilayer small molecule organic light emitting diodes without any dopant were fabricated in the following sequence: Indium tin oxide/4,4′-bis(N-(1-naphthyl)-N-phenylamino)biphenyl (50 nm)/5a–d (30 nm)/4,7-diphenyl-1,10-phenanthroline (30 nm)/LiF/Al. The electroluminescent property of the device fabricated with 5d as an emitter exhibited a high external quantum efficiency of 3.80% (at around 1 mA/cm{sup 2}) with Commission Internationale De L'Eclairage coordinates of (0.14, 0.25). - Highlights: • Synthesis and characterization of 2-(10-aryl)anthracen-9-yl)pyridine derivatives • Thermal, photophysical and electrochemical properties of anthracene derivatives • Emitters from blue to greenish blue for organic light emitting device applications • Organic light emitting device fabrication and characterization of 2-(10-aryl)anthracen-9-yl)pyridine derivatives.

  14. Deriving remote sensing reflectance from turbid Case II waters using green-shortwave infrared bands based model

    Science.gov (United States)

    Chen, Jun; Yin, Shoujing; Xiao, Rulin; Xu, Qianxiang; Lin, Changsong

    2014-04-01

    The objectives of this study are to validate the applicability of a shortwave infrared atmospheric correction model (SWIR-based model) in deriving remote sensing reflectance in turbid Case II waters, and to improve that model using a proposed green-shortwave infrared model (GSWIR-based model). In a GSWIR-based model, the aerosol type is determined by a SWIR-based model and the reflectance due to aerosol scattering is calculated using spectral slope technology. In this study, field measurements collected from three independent cruises from two different Case II waters were used to compare models. The results indicate that both SWIR- and GSWIR-based models can be used to derive the remote sensing reflectance at visible wavelengths in turbid Case II waters, but GSWIR-based models are superior to SWIR-based models. Using the GSWIR-based model decreases uncertainty in remote sensing reflectance retrievals in turbid Case II waters by 2.6-12.1%. In addition, GSWIR-based model’s sensitivity to user-supplied parameters was determined using the numerical method, which indicated that the GSWIR-based model is more sensitive to the uncertainty of spectral slope technology than to that of aerosol type retrieval methodology. Due to much lower noise tolerance of GSWIR-based model in the blue and near-infrared regions, the GSWIR-based model performs poorly in determining remote sensing reflectance at these wavelengths, which is consistent with the GSWIR-based model’s accuracy evaluation results.

  15. Schiff Base Metal Derivatives Enhance the Expression of HSP70 and Suppress BAX Proteins in Prevention of Acute Gastric Lesion

    Directory of Open Access Journals (Sweden)

    Shahram Golbabapour

    2013-01-01

    Full Text Available Schiff base complexes have appeared to be promising in the treatment of different diseases and disorders and have drawn a lot of attention to their biological activities. This study was conducted to evaluate the regulatory effect of Schiff base metal derivatives on the expression of heat shock proteins (HSP 70 and BAX in protection against acute haemorrhagic gastric ulcer in rats. Rats were assigned to 6 groups of 6 rats: the normal control (Tween 20 5% v/v, 5 mL/kg, the positive control (Tween 20 5% v/v, 5 mL/kg, and four Schiff base derivative groups named Schiff_1, Schiff_2, Schiff_3, and Schiff_4 (25 mg/kg. After 1 h, all of the groups received ethanol 95% (5 mL/kg but the normal control received Tween 20 (Tween 20 5% v/v, 5 mL/kg. The animals were euthanized after 60 min and the stomachs were dissected for histology (H&E, immunohistochemistry, and western blot analysis against HSP70 and BAX proteins. The results showed that the Schiff base metal derivatives enhanced the expression of HSP70 and suppressed the expression of BAX proteins during their gastroprotection against ethanol-induced gastric lesion in rats.

  16. Comparison of relative renal function measured with either 99m Tc-DTPA or 99m Tc-EC dynamic scintigraphies with that measured with 99m Tc-DMSA static scintigraphy

    Energy Technology Data Exchange (ETDEWEB)

    Domingues, F.C.; Fujikawa, G.Y.; Decker, H.; Alonso, G.; Pereira, J.C.; Duarte, P.S. [Centro de Diagnostico Fleury, Sao Paulo, SP (Brazil). Secao de Medicina Nuclear; Sao Paulo Univ. (USP), SP (Brazil). Escola de Saude Publica. Dept. de Epidemiologia]. E-mail: paulo.duarte@fleury.com.br

    2006-07-15

    Objective: The aim of this study was to compare the renal function measured with either {sup 99m}Tc-DTPA or {sup 99m}Tc-EC dynamic scintigraphies with that measured using {sup 99m}Tc-DMSA static scintigraphy. Methods: the values of relative renal function measured in 111 renal dynamic scintigraphies performed either with {sup 99m}Tc-DTPA (55 studies) or with {sup 99m}Tc-EC (56 studies) were compared with the relative function measured using {sup 99m}Tc-DMSA static scintigraphy performed within a 1-month period. The comparisons were performed using Wilcoxon signed rank test. The number of {sup 99m}Tc-DTPA and {sup 99m}Tc-EC studies that presented relative renal function different by more than 5% from that measured with {sup 99m}Tc-DMSA, using chi square test were also compared. Results: the relative renal function measured with {sup 99m}Tc-EC is not statistically different from that measured with {sup 99m}Tc-DMSA (p = 0.97). The relative renal function measured with {sup 99m}Tc-DTPA was statistically different from that measured using {sup 99m}Tc-DMSA, but with a borderline statistical significance (p = 0.05). The number of studies with relative renal function different by more than 5% from that measured with {sup 99m}Tc-DMSA is higher for the {sup 99m}Tc-DTPA scintigraphy (p 0.04) than for {sup 99m}Tc-EC. Conclusion: the relative renal function measured with {sup 99m}Tc-EC dynamic scintigraphy is comparable with that measured with {sup 99m}Tc-DMSA static scintigraphy, while the relative renal function measured with {sup 99m}Tc-DTPA dynamic scintigraphy presents a significant statistical difference from that measured with {sup 99m}Tc-DMSA static scintigraphy. (author)

  17. Comparison of relative renal function measured with either 99mTc-DTPA or 99mTc-EC dynamic scintigraphies with that measured with 99mTc-DMSA static scintigraphy

    Directory of Open Access Journals (Sweden)

    F. C. Domingues

    2006-08-01

    Full Text Available OBJECTIVE: The aim of this study was to compare the renal function measured with either 99mTc-DTPA or 99mTc-EC dynamic scintigraphies with that measured using 99mTc-DMSA static scintigraphy. METHODS: the values of relative renal function measured in 111 renal dynamic scintigraphies performed either with 99mTc-DTPA (55 studies or with 99mTc-EC (56 studies were compared with the relative function measured using 99mTc-DMSA static scintigraphy performed within a 1-month period. The comparisons were performed using Wilcoxon signed rank test. The number of 99mTc-DTPA and 99mTc-EC studies that presented relative renal function different by more than 5% from that measured with 99mTc-DMSA, using chi square test were also compared. RESULTS: the relative renal function measured with 99mTc-EC is not statistically different from that measured with 99mTc-DMSA (p = 0.97. The relative renal function measured with 99mTc-DTPA was statistically different from that measured using 99mTc-DMSA, but with a borderline statistical significance (p = 0.05. The number of studies with relative renal function different by more than 5% from that measured with 99mTc-DMSA is higher for the 99mTc-DTPA scintigraphy (p = 0.04 than for 99mTc-EC. CONCLUSION: the relative renal function measured with 99mTc-EC dynamic scintigraphy is comparable with that measured with 99mTc-DMSA static scintigraphy, while the relative renal function measured with 99mTc-DTPA dynamic scintigraphy presents a significant statistical difference from that measured with 99mTc-DMSA static scintigraphy.

  18. Safety and reactogenicity of the combined diphtheria-tetanus-acellular pertussis-inactivated poliovirus-Haemophilus influenzae type b (DTPa-IPV/Hib) vaccine in healthy Vietnamese toddlers: An open-label, phase III study.

    Science.gov (United States)

    Anh, Dang Duc; Van Der Meeren, Olivier; Karkada, Naveen; Assudani, Deepak; Yu, Ta-Wen; Han, Htay Htay

    2016-03-03

    The introduction of combination vaccines plays a significant role in increasing vaccine acceptance and widening vaccine coverage. Primary vaccination against diphtheria, tetanus, pertussis, poliomyelitis and Haemophilus influenza type b (Hib) diseases has been implemented in Vietnam. In this study we evaluated the safety and reactogenicity of combined diphtheria-tetanus-pertussis-inactivated polio (DTPa-IPV)/Hib vaccine when administered as a booster dose in 300 healthy Vietnamese children vaccine. DTPa-IPV/Hib conjugate vaccine was well tolerated as a booster dose in healthy Vietnamese children aged <2 years.

  19. Functionality of albumin-derived perfluorocarbon-based artificial oxygen carriers in the Langendorff-heart (†).

    Science.gov (United States)

    Wrobeln, Anna; Schlüter, Klaus D; Linders, Jürgen; Zähres, Manfred; Mayer, Christian; Kirsch, Michael; Ferenz, Katja B

    2017-06-01

    The aim of this study was to prove whether albumin-derived perfluorocarbon-based nanoparticles (capsules) can operate as a novel artificial oxygen carrier in a rat Langendorff-heart perfusion model. Hearts perfused with capsules showed increased left ventricular pressure and rate pressure product compared to hearts perfused with pure Krebs-Henseleit (KH)-buffer. The capsules prevented the myocardium from functional fail when in their absence a noxious ischemia was observed. Capsules did not change rheological properties of KH-buffer and could repeatedly reload with oxygen. This albumin-derived perfluorocarbon-based artificial oxygen carrier preserved the function of rat hearts due to the transport of oxygen in a satisfactory manner. Because of these positive results, the functionality of the applied capsules should be verified in living animals.

  20. Semisynthesis and quantitative structure-activity relationship (QSAR) study of some cholesterol-based hydrazone derivatives as insecticidal agents.

    Science.gov (United States)

    Yang, Chun; Shao, Yonghua; Zhi, Xiaoyan; Huan, Qu; Yu, Xiang; Yao, Xiaojun; Xu, Hui

    2013-09-01

    In continuation of our program aimed at the discovery and development of natural-product-based insecticidal agents, four series of novel cholesterol-based hydrazone derivatives were synthesized, and their insecticidal activity was tested against the pre-third-instar larvae of oriental armyworm, Mythimna separata (Walker) in vivo at 1mg/mL. All the derivatives showed the better insecticidal activity than their precursor cholesterol. Quantitative structure-activity relationship (QSAR) model demonstrated that six descriptors such as RDF085v, Mor06u, Mor11u, Dv, HATS0v and H-046, are likely to influence the insecticidal activity of these compounds. Among them, two important ones are the Mor06u and RDF085v.

  1. Synthesis, crystal structures, fluorescence and xanthine oxidase inhibitory activity of pyrazole-based 1,3,4-oxadiazole derivatives

    Science.gov (United States)

    Qi, De-Qiang; Yu, Chuan-Ming; You, Jin-Zong; Yang, Guang-Hui; Wang, Xue-Jie; Zhang, Yi-Ping

    2015-11-01

    A series of pyrazole-based 1,3,4-oxadiazole derivatives were rationally designed and synthesized in good yields by following a convenient route. All the newly synthesized molecules were fully characterized by IR, 1H NMR and elemental analysis. Eight compounds were structurally determined by single crystal X-ray diffraction analysis. The fluorescence properties of all the compounds were investigated in dimethyl sulfoxide media. In addition, these newly synthesized compounds were evaluated for in vitro inhibitory activity against commercial enzyme xanthine oxidase (XO) by measuring the formation of uric acid from xanthine. Among the compounds synthesized and tested, 3d and 3e were found to be moderate inhibitory activity against commercial XO with IC50 = 72.4 μM and 75.6 μM. The studies gave a new insight in further optimization of pyrazole-based 1,3,4-oxadiazole derivatives with excellent fluorescence properties and XO inhibitory activity.

  2. Synthesis and antimicrobial activities of new higher amino acid Schiff base derivatives of 6-aminopenicillanic acid and 7-aminocephalosporanic acid

    Science.gov (United States)

    Özdemir (nee Güngör), Özlem; Gürkan, Perihan; Özçelik, Berrin; Oyardı, Özlem

    2016-02-01

    Novel β-lactam derivatives (1c-3c) (1d-3d) were produced by using 6-aminopenicillanic acid (6-APA), 7-aminocephalosporanic acid (7-ACA) and the higher amino acid Schiff bases. The synthesized compounds were characterized by elemental analysis, IR, 1H/13C NMR and UV-vis spectra. Antibacterial activities of all the higher amino acid Schiff bases (1a-3a) (1b-3b) and β-lactam derivatives were screened against three gram negative bacteria (Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Acinetobacter baumannii RSKK 02026), three gram positive bacteria (Staphylococcus aureus ATCC 25923, Enterococcus faecalis ATCC 07005, Bacillus subtilis ATCC 6633) and their drug-resistant isolates by using broth microdilution method. Two fungi (Candida albicans and Candida krusei) were used for antifungal activity.

  3. Highly selective fluorescent chemosensor for Na+ based on pyrene-modified calix[4]arene derivative

    Institute of Scientific and Technical Information of China (English)

    WANG KeRang; GUO DongSheng; JIANG BangPing; LIU Yu

    2009-01-01

    A novel calix[4]arene derivative with pyrene fluorophores at the upper rim and tetraester ionophores at the lower rim was synthesized in six steps, and its structure was proved by NMR and ESi-MS spectro-scopies. Furthermore, the chemosensing behavior of the host compound for alkali and alkaline earth metal ions was investigated by fluorescence spectroscopy. The obtained results show that the calix-arene host can selectively bind sodium ion with the complexation stability constant of 2190 mol-1.L. The complexation with sodium ion can pronouncedly induce the excimer emission to decrease and the monomer emission to increase, whereas the addition of the other alkali and alkaline earth metal ions does not cause appreciable changes in the fluorescence spectrum of the host compound. The present calix[4]arene derivative displays potential application as fluorescent chemosensor for sodium ion.

  4. Global detailed gravimetric geoid. [based on gravity model derived from satellite tracking and surface gravity data

    Science.gov (United States)

    Vincent, S.; Marsh, J. G.

    1973-01-01

    A global detailed gravimetric geoid has been computed by combining the Goddard Space Flight Center GEM-4 gravity model derived from satellite and surface gravity data and surface 1 deg-by-1 deg mean free air gravity anomaly data. The accuracy of the geoid is + or - 2 meters on continents, 5 to 7 meters in areas where surface gravity data are sparse, and 10 to 15 meters in areas where no surface gravity data are available. Comparisons have been made with the astrogeodetic data provided by Rice (United States), Bomford (Europe), and Mather (Australia). Comparisons have also been carried out with geoid heights derived from satellite solutions for geocentric station coordinates in North America, the Caribbean, Europe, and Australia.

  5. A Topological derivative based image segmentation for sign language recognition system using isotropic filter

    CERN Document Server

    Krishnaveni, M

    2010-01-01

    The need of sign language is increasing radically especially to hearing impaired community. Only few research groups try to automatically recognize sign language from video, colored gloves and etc. Their approach requires a valid segmentation of the data that is used for training and of the data that is used to be recognized. Recognition of a sign language image sequence is challenging because of the variety of hand shapes and hand motions. Here, this paper proposes to apply a combination of image segmentation with restoration using topological derivatives for achieving high recognition accuracy. Image quality measures are conceded here to differentiate the methods both subjectively as well as objectively. Experiments show that the additional use of the restoration before segmenting the postures significantly improves the correct rate of hand detection, and that the discrete derivatives yields a high rate of discrimination between different static hand postures as well as between hand postures and the scene b...

  6. Mismatch base pairing of the mutagen 8-oxoguanine and its derivatives with adenine: A theoretical search for possible antimutagenic agents

    Science.gov (United States)

    Singh, A. K.; Mishra, P. C.

    Molecular geometries of 8-oxoguanine (8OG), those of its substituted derivatives with the substitutions CH2, CF2, CO, CNH, O, and S in place of the N7H7 group, adenine (A), and the base pairs of 8OG and its substituted derivatives with adenine were optimized using the RHF/6-31+G* and B3LYP/6-31+G* methods in gas phase. All the molecules and their hydrogen-bonded complexes were solvated in aqueous media employing the polarized continuum model (PCM) of the self-consistent reaction field (SCRF) theory using the RHF/6-31+G* and B3LYP/6-31+G* methods. The optimized geometrical parameters of the 8OG-A base pair at the RHF/6-31+G* and B3LYP/6-31+G* levels of theory agree satisfactorily with those of an oligonucleotide containing the base pair found from X-ray crystallography. The pattern of hydrogen bonding in the CF2- and O-substituted 8OG-A base pair is of Watson-Crick type and that in the unsubstituted and CH2-, CNH-, and S-substituted base pairs is of Hoogsteen type. In the CO-substituted base pair, the hydrogen bonding pattern is of neither Watson-Crick nor Hoogsteen type. The CF2-substitution appears to introduce steric hindrance for stacking of DNA bases. On the basis of these results, it appears that among all the substituted 8OG molecules considered here, the O-substituted derivative may be useful as an antimutagenic drug. It is, however, subject to experimental verification. Content:text/plain; charset="UTF-8"

  7. Derivation of Exact Eigenvalues and Eigenfunctions Based on the Analytical Transfer Matrix Method

    Institute of Scientific and Technical Information of China (English)

    HE Ying; CAO Zhuang-Qi; SHEN Qi-Shun

    2004-01-01

    @@ We extend the analytical transfer matrix method (ATMM) to calculate both the energy eigenvalues and wavefunctions for the Morse potential and the regulated Coulomb potential. Derivations of the exact eigenenergies and eigenfunctions are presented in detail by the ATMM. We compare our results with that obtained by relaxational approach and the eigenvalue moment method, and it is shown that the ATMM can produce accurate eigenvalues.The eigenfunctions by the ATMM are also proven to be correct and meaningful.

  8. A General Class of Derivative Free Optimal Root Finding Methods Based on Rational Interpolation

    Directory of Open Access Journals (Sweden)

    Fiza Zafar

    2015-01-01

    Full Text Available We construct a new general class of derivative free n-point iterative methods of optimal order of convergence 2n-1 using rational interpolant. The special cases of this class are obtained. These methods do not need Newton’s iterate in the …first step of their iterative schemes. Numerical computations are presented to show that the new methods are efficient and can be seen as better alternates.

  9. Activity Coefficient Derivatives of Ternary Systems Based on Scatchard's Neutral Electrolyte description

    Energy Technology Data Exchange (ETDEWEB)

    Miller, D G

    2007-05-16

    Activity coefficient derivatives with respect to molality are presented for the Scatchard Neutral Electrolyte description of a ternary common-ion electrolyte system. These quantities are needed for the calculation of 'diffusion Onsager coefficients' and in turn for tests of the Onsager Reciprocal Relations in diffusion. The usually-omitted b{sub 23} term is included. The direct SNE binary approximations and a further approximation are discussed. Binary evaluation strategies other than constant ionic strength are considered.

  10. Highly Selective Fluoride Recognition by a Simple Carbazole-based Hydrozone Derivative

    Institute of Scientific and Technical Information of China (English)

    TANG Li-jun; ZHAO Guo-you; HUANG Zhen-long; WANG Nan-nan; GUO Jiao-jiao

    2013-01-01

    A simple carbazole hydrozone derivative(1) was synthesized and used as an optical probe for fluoride recognition in CH3CN.Receptor 1 exhibited colorimetric and fluorescent dual-channel response to fluoride anions.Addition of fluoride to a receptor 1 solution in CH3CN induced a distinct color change from yellow to magenta,the solution also displayed significant fluorescence blue shift.Thus receptor 1 exhibited dual-channel responses to fluoride with a high selectivity and sensitivity.

  11. Synthesis and Antiproliferative Activities of Benzimidazole-Based Sulfide and Sulfoxide Derivatives.

    Science.gov (United States)

    Gaballah, Samir T; El-Nezhawy, Ahmed O H; Amer, Hassan; Ali, Mamdouh Moawad; Mahmoud, Abeer Essam El-Din; Hofinger-Horvath, Andreas

    2016-01-01

    The design, synthesis, and in vitro antiproliferative activity of a novel series of sulfide (4a-i) and sulfoxide (5a-h) derivatives of benzimidazole, in which different aromatic and heteroaromatic acetamides are linked to benzimidazole via sulfide (4a-i) and sulfoxide (5a-h) linker, are reported and the structure-activity relationship is discussed. The new derivatives were prepared by coupling 2-(mercaptomethyl)benzimidazole with 2-bromo-N-(substituted) acetamides in dry acetone in the presence of anhydrous potassium carbonate. With very few exceptions, all of the synthesized compounds showed varying antiprolific activities against HepG2, MCF-7, and A549 cell lines. Compound 5a was very similar in potency to doxorubicin as an anticancer drug, with IC50 values 4.1 ± 0.5, 4.1 ± 0.5, and 5.0 ± 0.6 µg/mL versus 4.2 ± 0.5, 4.9 ± 0.6, and 6.1 ± 0.6 µg/mL against HepG2, MCF-7, and A549 cell lines, respectively. In contrast, none of the compounds showed activity against human prostate PC3 cancer cells. Additionally, the sulfoxide derivatives were more potent than the corresponding sulfides.

  12. Turn-On Fluorescent Chemosensor for Fluoride Based on Pyreneamide Derivative

    Energy Technology Data Exchange (ETDEWEB)

    Jeon, Nam Joong; Hong, Sung Won; Hong, Ju Hyun; Jeong, Ju Mi; Nam, Kye Chun [Seoul National University, Seoul (Korea, Republic of)

    2012-01-15

    A new chemosensor with pyreneamide derivative of bipyridine is synthesized. In the free ligand, pyreneamide derivative has nearly no fluorescence in acetonitrile solution. However, in the presence of fluoride ion, a 'turn-on' fluorescence was observed. Simultaneously, the colorless ligand solution became markedly orange when fluoride ion was added to pyreneamide derivative in acetonitrile. This phenomenon suggest that the PET (photoinduced electron transfer) between anion electron and pyrene unit was changed the π-π interaction between bipyridine and pyrene that was modified structure by deprotonation. On account of the important roles of anion in biological, clinical, environmental, catalysis, and chemical processes, the selective and efficient recognition of anion is an area of growing interest in supramolecular chemistry. In particular, the studies of chemosensors toward F{sup -} anion are quite intriguing because of its beneficial effects in human physiology. Also, fluoride is interest due to its established role in dental care and osteoporosis. However, an excess of fluoride ion can lead to fluorosis. Therefore, the development of reliable sensors for F{sup -} is needed for environment and human health care. Color changes that can be detected by the naked eye are widely used as signals for events owing to the inexpensive equipment required or no equipment at all.

  13. Determining quasidiabatic coupled electronic state Hamiltonians using derivative couplings: A normal equations based method.

    Science.gov (United States)

    Papas, Brian N; Schuurman, Michael S; Yarkony, David R

    2008-09-28

    A self-consistent procedure for constructing a quasidiabatic Hamiltonian representing N(state) coupled electronic states in the vicinity of an arbitrary point in nuclear coordinate space is described. The matrix elements of the Hamiltonian are polynomials of arbitrary order. Employing a crude adiabatic basis, the coefficients of the linear terms are determined exactly using analytic gradient techniques. The remaining polynomial coefficients are determined from the normal form of a system of pseudolinear equations, which uses energy gradient and derivative coupling information obtained from reliable multireference configuration interaction wave functions. In a previous implementation energy gradient and derivative coupling information were employed to limit the number of nuclear configurations at which ab initio data were required to determine the unknown coefficients. Conversely, the key aspect of the current approach is the use of ab initio data over an extended range of nuclear configurations. The normal form of the system of pseudolinear equations is introduced here to obtain a least-squares fit to what would have been an (intractable) overcomplete set of data in the previous approach. This method provides a quasidiabatic representation that minimizes the residual derivative coupling in a least-squares sense, a means to extend the domain of accuracy of the diabatic Hamiltonian or refine its accuracy within a given domain, and a way to impose point group symmetry and hermiticity. These attributes are illustrated using the 1 (2)A(1) and 1 (2)E states of the 1-propynyl radical, CH(3)CC.

  14. Image Prediction Method with Nonlinear Control Lines Derived from Kriging Method with Extracted Feature Points Based on Morphing

    Directory of Open Access Journals (Sweden)

    Kohei Arai

    2013-01-01

    Full Text Available Method for image prediction with nonlinear control lines which are derived from extracted feature points from the previously acquired imagery data based on Kriging method and morphing method is proposed. Through comparisons between the proposed method and the conventional linear interpolation and widely used Cubic Spline interpolation methods, it is found that the proposed method is superior to the conventional methods in terms of prediction accuracy.

  15. NMR study of the preparation of 6 {alpha}, 7 {beta}-dihydroxyvouacapan-17 beta-oic acid mannich base derivatives

    Energy Technology Data Exchange (ETDEWEB)

    Santos, Flavio Jose Leite dos; Pilo-Veloso, Dorila [Universidade Federal de Minas Gerais (UFMG), Belo Horizonte (Brazil). Inst. de Ciencias Exatas. Dept. Quimica]. E-mail: dorila@zeus.qui.ufmg.br; Ferreira-Alves, Dalton L. [Universidade Federal de Minas Gerais (UFMG), Belo Horizonte (Brazil). Inst. de Ciencias Biologicas. Dept. de Farmacologia

    2007-07-01

    This work presents four new Mannich base compounds obtained by the Mannich reaction of a {delta}-keto-lactone derivative of 6{alpha}, 7{beta}-dihydroxyvouacapan- 17{beta}-oic acid, a furano diterpene isolated from the hexane extract of Pterodon polygalaeflorus Benth fruits, which shows anti-inflammatory and analgesic activities. The use of 1D and 2D NMR (COSY, DEPT-135, HMBC, HMQC) spectroscopy made it possible to characterize the new compounds. (author)

  16. Acrosin structure-based design, synthesis and biological activities of 7-azaindol derivatives as new acrosin inhibitors

    Institute of Scientific and Technical Information of China (English)

    Jun Hang Jiang; Xue Fei Liu; Can Hui Zhen; You Jun Zhou; Ju Zhu; Jia Guo Lv; Chun Quan Sheng

    2011-01-01

    A series of 7-azaindol derivatives were designed based on the homologous 3D model of human acrosin. These compounds were synthesized and evaluated for their human acrosin inhibitory activities in vitro. Compounds 7a, 7i, 7j, 7k and 7n showed highly inhibitory activity against human acrosin. The three-dimensional structure-activity relationship was investigated through a CoMFA model, which provided valuable information to further study of potential human acrosin inhibitors.

  17. Fluorescence turn-on chemosensor for Hg2+ based on a rhodamine derivative and its application in bioimaging

    Institute of Scientific and Technical Information of China (English)

    2009-01-01

    A fluorescence turn-on chemosensor based on rhodamine B derivative (FD10) has been developed as a highly sensitive chemosensor for Hg2+. A prominent fluorescence enhancement was measured in the presence of Hg2+, which was in agreement with the changes in the absorption spectrum. Furthermore, by means of laser scanning fluorescence microscopy experiments, it was demonstrated that FD10 was cell-permeable and could be used as a fluorescent probe for monitoring Hg2+ in living cells.

  18. Discovery and Development of Natural Product-derived Chemotherapeutic Agents Based on a Medicinal Chemistry Approach⊥†

    OpenAIRE

    Lee, Kuo-Hsiung

    2010-01-01

    Medicinal plants have long been an excellent source of pharmaceutical agents. Accordingly, the long term objectives of the author's research program are to discover and design new chemotherapeutic agents based on plant-derived compound leads by using a medicinal chemistry approach, which is a combination of chemistry and biology. Different examples of promising bioactive natural products and their synthetic analogs, including sesquiterpene lactones, quassinoids, naphthoquinones, phenylquinolo...

  19. Biokinetics and dosimetry of {sup 111}In-DOTA-NOC-ATE compared with {sup 111}In-DTPA-octreotide

    Energy Technology Data Exchange (ETDEWEB)

    Boubaker, Ariane; Prior, John O.; Champendal, Melanie; Bischof Delaloye, Angelika [Lausanne University Hospital, CHUV, Department of Nuclear Medicine, Lausanne (Switzerland); Willi, Jean-Pierre [University Hospital of Geneva, Department of Nuclear Medicine, Geneva 14 (Switzerland); Kosinski, Marek; Baechler, Sebastien [Lausanne University Hospital, Institute of Radiation Physics, Lausanne (Switzerland); Maecke, Helmut R. [University Hospital of Freiburg, Department of Nuclear Medicine, Freiburg (Germany); Ginj, Mihaela [University Health Network, Joint Department of Medical Imaging, Toronto, ON (Canada); Buchegger, Franz [Lausanne University Hospital, CHUV, Department of Nuclear Medicine, Lausanne (Switzerland); University Hospital of Geneva, Department of Nuclear Medicine, Geneva 14 (Switzerland)

    2012-12-15

    The biokinetics and dosimetry of {sup 111}In-DOTA-NOC-ATE (NOCATE), a high-affinity ligand of SSTR-2 and SSTR-5, and {sup 111}In-DTPA-octreotide (Octreoscan trademark, OCTREO) were compared in the same patients. Seventeen patients (10 men, 7 women; mean age 60 years), referred for an OCTREO scan for imaging of a neuroendocrine tumour (15), thymoma (1) or medullary thyroid carcinoma (1), agreed to undergo a second study with NOCATE. Whole-body anterior-posterior scans were recorded 0.5 (100 % reference scan), 4, 24 and 48 h (17 patients) and 120 h (5 patients) after injection. In 16 patients the OCTREO scan (178 {+-} 15 MBq) was performed 16 {+-} 5 days before the NOCATE scan (108 {+-} 14 MBq) with identical timing; 1 patient had the NOCATE scan before the OCTREO scan. Blood samples were obtained from 14 patients 5 min to 48 h after injection. Activities expressed as percent of the initial (reference) activity in the whole body, lung, kidney, liver, spleen and blood were fitted to biexponential or single exponential functions. Dosimetry was performed using OLINDA/EXM. Initial whole-body, lung and kidney activities were similar, but retention of NOCATE was higher than that of OCTREO. Liver and spleen uptakes of NOCATE were higher from the start (p < 0.001) and remained so over time. Whole-body activity showed similar {alpha} and {beta} half-lives, but the {beta} fraction of NOCATE was double that of OCTREO. Blood T{sub 1/2}{beta} for NOCATE was longer (19 vs. 6 h). As a result, the effective dose of NOCATE (105 {mu}Sv/MBq) exceeded that of OCTREO (52 {mu}Sv/MBq), and the latter result was similar to the ICRP 106 value of 54 {mu}Sv/MBq. Differential activity measurement in blood cells and plasma showed an average of <5 % of NOCATE and OCTREO attached to globular blood components. NOCATE showed a slower clearance from normal tissues and its effective dose was roughly double that of OCTREO. (orig.)

  20. Determination of appropriate sampling frequency and time of multiple blood sampling dual exponential method with {sup 99m}Tc-DTPA for calculating GFR

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Chung Ho; O, Joo Hyun; Chung, Yong An; Yoo, Le Ryung; Sohn, Hyung Sun; Kim, Sung Hoon; Chung, Soo Kyo; Lee, Hyoung Koo [Catholic University of Korea, Seoul (Korea, Republic of)

    2006-02-15

    To determine appropriate sampling frequency and time of multiple blood sampling dual exponential method with {sup 99m}Tc-DTPA for calculating glomerular filtration rate (GFR). Thirty four patients were included in this study. Three mCi of {sup 99m}Tc-DTPA was intravenously injected and blood sampling at 9 different times, 5 ml each, were done. Using the radioactivity of serum, measured by gamma counter, the GFR was calculated using dual exponential method and corrected with the body surface area. Using spontaneously chosen 2 data points of serum radioactivity, 15 collections of 2-sample GFR were calculated. And 10 collections of 3-sample GFR and 12 collections of 4-sample GFR were also calculated. Using the 9-sample GFR as a reference value, degree of agreement was analyzed with Kendall's {tau} correlation coefficients, mean difference and standard deviation. Although some of the 2-sample GFR showed high correlation coefficient, over or underestimation had evolved as the renal function change. The 10-120-240 min 3-sample GFR showed a high correlation coefficient {tau} =0.93), minimal difference (Mean{+-}SD= -1.784{+-}3.972), and no over or underestimation as the renal function changed. Th 4-sample GFR showed no better accuracy than the 3-sample GFR. Int the wide spectrum or renal function, the 10-120-240 min 3-sample GFR could be the best choice for estimating the patients' renal function.

  1. Pre-surgical mapping of primary motor cortex by functional MRI at 3 T: effects of intravenous administration of Gd-DTPA

    Energy Technology Data Exchange (ETDEWEB)

    Naganawa, Shinji; Nihashi, Takashi; Fukatsu, Hiroshi; Ishigaki, Takeo [Department of Radiology, Nagoya University School of Medicine, 65 Tsurumai-cho, Shouwa-ku, 466-8550, Nagoya (Japan); Aoki, Ikuo [Toshiba Medical Systems Company, Tokyo (Japan)

    2004-01-01

    The functional magnetic resonance imaging (fMRI) is often performed at the end of a routine MRI examination during which, dependent on the clinical indication, contrast agent has been administered; however, the effects of Gd-DTPA injection on the results of blood oxygenation level dependent (BOLD)-fMRI remain unknown. The present study was conducted to investigate the effects of the intravenous administration of Gd-DTPA on the results of pre-surgical localization of the primary motor cortex by BOLD-fMRI at 3 T. Eight normal subjects were included in this study. After the anatomical scans, pre- and post-contrast fMRI scanning was performed. The number of significantly activated voxels and the mean percentage signal change were compared. The mean number of significantly activated voxels was 115.0{+-}27.0 in pre-contrast runs and 90.8{+-}27.1 in post-contrast runs (mean value of all 8 volunteers{+-}standard deviation; p<0.05). The mean mean percentage signal change was 4.07{+-}0.39 in pre-contrast runs and 3.86{+-}1.91 in post-contrast runs (p=0.16). Pre-surgical localization of the motor area by BOLD-fMRI should be performed before the administration of Gd contrast material. (orig.)

  2. Dynamic contrast-enhanced magnetic resonance imaging with Gd-EOB-DTPA for the evaluation of liver fibrosis in chronic hepatitis patients

    Energy Technology Data Exchange (ETDEWEB)

    Chen, Bang-Bin; Hsu, Chao-Yu.; Yu, Chih-Wei; Wei, Shwu-Yuan; Shih, Tiffany Ting-Fang [National Taiwan University College of Medicine and Hospital, Department of Radiology and Medical Imaging, Taipei (China); Kao, Jia-Horng [National Taiwan University College of Medicine and Hospital, Department of Internal Medicine, Taipei (China); National Taiwan University College of Medicine, Graduate Institute of Clinical Medicine, Taipei (China); Lee, Hsuan-Shu [National Taiwan University College of Medicine and Hospital, Department of Internal Medicine, Taipei (China)

    2012-01-15

    To develop a non-invasive MRI method for evaluation of liver fibrosis, with histological analysis as the reference standard. The study protocol was approved by the Institutional Review Board for Human Studies of our hospital, and written informed consent was obtained from all subjects. Seventy-nine subjects who received dynamic contrast-enhanced MRI (DCE-MRI) with Gd-EOB-DTPA were divided into three subgroups according to Metavir score: no fibrosis (n = 30), mild fibrosis (n = 34), and advanced fibrosis (n = 15). The DCE-MRI parameters were measured using two models: (1) dual-input single-compartment model for arterial blood flow (F{sub a}), portal venous blood flow, total liver blood flow, arterial fraction (ART), distribution volume, and mean transit time; and (2) curve analysis model for Peak, Slope, and AUC. Statistical analysis was performed with Student's t-test and the nonparametric Kruskal-Wallis test. Slope and AUC were two best perfusion parameters to predict the severity of liver fibrosis (>F2 vs. {<=}F2). Four significantly different variables were found between non-fibrotic versus mild-fibrotic subgroups: F{sub a}, ART, Slope, and AUC; the best predictor for mild fibrosis was F{sub a} (AUROC:0.701). DCE-MRI with Gd-EOB-DTPA is a noninvasive imaging, by which multiple perfusion parameters can be measured to evaluate the severity of liver fibrosis. (orig.)

  3. DISTRIBUIÇÃO DE CHUMBO NO PERFIL DE SOLO AVALIADA PELAS SOLUÇÕES DE DTPA E MEHLICH-3

    Directory of Open Access Journals (Sweden)

    ABREU CLEIDE APARECIDA DE

    1998-01-01

    Full Text Available Tendo em vista a possibilidade de aumentos significativos no conteúdo de Pb em solos cultivados perto de pólos industriais, procurou-se, no presente trabalho, avaliar a distribuição desse elemento no perfil de solo próximo e distante de uma fonte geradora, localizada entre a Rodovia Presidente Dutra e o núcleo urbano de Caçapava Velha (SP e comparar procedimentos analíticos para extração de Pb em solos. Coletaram-se as amostras de solo a 500 e 1.000 m da fonte geradora, em quatro direções (N, S, E e W e nas profundidades de 0-5, 5-10, 10-20, 20-40 e 0-20 cm. A extração de Pb foi feita com a solução de DTPA e de Mehlich-3 (M-3, sendo as mais altas concentrações de Pb encontradas nas amostras de solos localizadas próximo à fonte geradora. Verificou-se claramente que o Pb tendeu a acumular-se na camada superficial, diminuindo com a profundidade. Concluiu-se que as soluções de DTPA e de M-3 foram eficientes para avaliar a contaminação por Pb, podendo ser utilizadas no monitoramento do impacto ambiental causado por esse elemento.

  4. Physicochemical Studies of Some Schiff Bases Derived From 6-Ethylbenzene 1,3-diol

    OpenAIRE

    Shipra Baluja

    2004-01-01

    The schiff bases were synthesized and their characterization was done by CHN analysis, IR and NMR spectra. The physicochemical properties such as density, refractive index, conductance, heat of solution etc., of these schiff bases were determined

  5. Physicochemical Studies of Some Schiff Bases Derived From 6-Ethylbenzene 1,3-diol

    Directory of Open Access Journals (Sweden)

    Shipra Baluja

    2004-01-01

    Full Text Available The schiff bases were synthesized and their characterization was done by CHN analysis, IR and NMR spectra. The physicochemical properties such as density, refractive index, conductance, heat of solution etc., of these schiff bases were determined

  6. Synthesis of 4-aminoantipyrine derived Schiff bases and their evaluation for antibacterial, cytotoxic and free radical scavenging activity

    Directory of Open Access Journals (Sweden)

    Mohammad Shoaib

    2015-06-01

    Full Text Available The work was aimed to synthesize 4-Aminoantipyrine derived Schiff bases in economical way and to screen it for study the effect of nitro group on its antibacterial potential, conduct antitumor preliminary study, the effects of group presence in benzylidene phenyl ring on the cytotoxic potentials and study the effects of electron withdrawing and donating group on antioxidant potential. We used green method with 75% reduction in general synthesis time of Schiff bases. Synthesized compound possess antibacterial potentials and nitro group presence enhances this potential. G2, G3, G4, G5, G6, G7 and G8 have significant cytotoxic and no significant antioxidant activity.

  7. Catalytic Oxidative Decarboxylation of Some Benzylcarboxylic Acid Derivatives by a New Iron(Ill) Schiff Base Complex

    Institute of Scientific and Technical Information of China (English)

    MONTAZEROZOHORI,Morteza; NASR-ESFAHANI,Masoud; AKHLAGHI,Parisa

    2009-01-01

    A quadridentate Schiff base ligand of N,N'-bis(2-hydroxy-a-methylbenzylidene)ethylenediamine (HMBEDA) and its new iron(Ⅲ) complex were synthesized and identified by analytical, spectral data (1H NMR, 13C NMR FT-IR and UV-visible) and molar conductance. A rapid and efficient homogeneous oxidative decarboxylatio n ofsome benzylcarboxylic acid derivatives was carried out by a catalytic amount of iron(Ⅲ) Schiff base complex in chloroform, using tetrabutylammonium periodate as a mild oxidant in good to excellent yields at room temperature.

  8. Synthesis and insecticidal activity of new oxime derivatives of podophyllotoxin-based phenazines against Mythimna separata Walker.

    Science.gov (United States)

    Zhi, Xiaoyan; Yang, Chun; Yu, Xiang; Xu, Hui

    2014-12-15

    To discover new natural-product-based insecticidal agents, a series of novel oxime derivatives of podophyllotoxin-based phenazines modified in the C, D and E rings of podophyllotoxin were prepared and tested as insecticidal agents against the pre-third-instar larvae of oriental armyworm, Mythimna separata (Walker) in vivo at 1 mg/mL. The steric configuration of IIIc was unambiguously confirmed by single-crystal X-ray diffraction analysis. Compounds IIIa-d, and IIIi exhibited an equal or higher insecticidal activity than toosendanin.

  9. Selection of appropriate isolation method based on morphology of blastocyst for efficient derivation of buffalo embryonic stem cells.

    Science.gov (United States)

    Kumar, R; Ahlawat, S P S; Sharma, M; Verma, O P; Sai Kumar, G; Taru Sharma, G

    2014-03-01

    The efficiency of embryonic stem cell (ESC) derivation from all species except for rodents and primates is very low. There are however, multiple interests in obtaining pluripotent cells from these animals with main expectations in the fields of transgenesis, cloning, regenerative medicine and tissue engineering. Researches are being carried out in laboratories throughout the world to increase the efficiency of ESC isolation for their downstream applications. Thus, the present study was undertaken to study the effect of different isolation methods based on the morphology of blastocyst for efficient derivation of buffalo ESCs. Embryos were produced in vitro through the procedures of maturation, fertilization and culture. Hatched blastocysts or isolated inner cell masses (ICMs) were seeded on mitomycin-C inactivated buffalo fetal fibroblast monolayer for the development of ESC colonies. The ESCs were analyzed for alkaline phosphatase activity, expression of pluripotency markers and karyotypic stability. Primary ESC colonies were obtained after 2-5 days of seeding hatched blastocysts or isolated ICMs on mitomycin-C inactivated feeder layer. Mechanically isolated ICMs attached and formed primary cell colonies more efficiently than ICMs isolated enzymatically. For derivation of ESCs from poorly defined ICMs intact hatched blastocyst culture was the most successful method. Results of this study implied that although ESCs can be obtained using all three methods used in this study, efficiency varies depending upon the morphology of blastocyst and isolation method used. So, appropriate isolation method must be selected depending on the quality of blastocyst for efficient derivation of ESCs.

  10. The effect of choosing three different C factor formulae derived from NDVI on a fully raster-based erosion modelling

    Science.gov (United States)

    Sulistyo, Bambang

    2016-11-01

    The research was aimed at studying the efect of choosing three different C factor formulae derived from NDVI on a fully raster-based erosion modelling of The USLE using remote sensing data and GIS technique. Methods applied was by analysing all factors affecting erosion such that all data were in the form of raster. Those data were R, K, LS, C and P factors. Monthly R factor was evaluated based on formula developed by Abdurachman. K factor was determined using modified formula used by Ministry of Forestry based on soil samples taken in the field. LS factor was derived from Digital Elevation Model. Three C factors used were all derived from NDVI and developed by Suriyaprasit (non-linear) and by Sulistyo (linear and non-linear). P factor was derived from the combination between slope data and landcover classification interpreted from Landsat 7 ETM+. Another analysis was the creation of map of Bulk Density used to convert erosion unit. To know the model accuracy, model validation was done by applying statistical analysis and by comparing Emodel with Eactual. A threshold value of ≥ 0.80 or ≥ 80% was chosen to justify. The research result showed that all Emodel using three formulae of C factors have coeeficient of correlation value of > 0.8. The results of analysis of variance showed that there was significantly difference between Emodel and Eactual when using C factor formula developed by Suriyaprasit and Sulistyo (non-linear). Among the three formulae, only Emodel using C factor formula developed by Sulistyo (linear) reached the accuracy of 81.13% while the other only 56.02% as developed by Sulistyo (nonlinear) and 4.70% as developed by Suriyaprasit, respectively.

  11. Integral Theorems Based on a New Gradient Operator Derived from Biomembranes (Part ⅠⅡ): Fundamentals

    Institute of Scientific and Technical Information of China (English)

    YIN Yajun

    2005-01-01

    A new gradient operator was derived in recent studies of topological structures and shape transitions in biomembranes. Because this operator has widespread potential uses in mechanics, physics, and biology, the operator's general mathematical characteristics should be investigated. This paper explores the integral characteristics of the operator. The second divergence and the differential properties of the operator are used to demonstrate new integral transformations for vector and scalar fields on curved surfaces, such as the second divergence theorem, the second gradient theorem, the second curl theorem, and the second circulation theorem. These new theorems provide a mathematical basis for the use of this operator in many disciplines.

  12. Novel Chromogenic Chemosensors for Fluoride Anion Based on 8-Hydroxyquinoline Azo Derivatives

    Institute of Scientific and Technical Information of China (English)

    CHENG,Yun-Fei; LIU,Zhi-Qiang; SHI,Mei; ZHAO,Qiang; LI,Fu-You; YI,Tao; HUANG,Chun-Hui

    2007-01-01

    A series of 8-hydroxyquinoline azo derivatives with diverse conjugated structures were synthesized and studied to chromogenically detect anions. All the dyes allowed selective detection for fluoride anion in CH3CN via instant deprotonation of the compounds, which was affirmed by UV-Vis absorption and 1H NMR spectra. The chromogenically responding ability increases as the substituent changes from phenyl to naphthyl or anthryl. This result is likely to be related to the enhancement of intramolecular charge transfer (ICT) induced by extension of conjugated structure.

  13. Field-effect and capacitive properties of water-gated transistors based on polythiophene derivatives

    Directory of Open Access Journals (Sweden)

    R. Porrazzo

    2015-01-01

    Full Text Available Recently, water-gated organic field-effect transistors (WGOFET have been intensively studied for their application in the biological field. Surprisingly, a very limited number of conjugated polymers have been reported so far. Here, we systematically explore a series of polythiophene derivatives, presenting different alkyl side chains lengths and orientation, and characterized by various morphologies: comparative evaluation of their performances allows highlighting the critical role played by alkyl side chains, which significantly affects the polymer/water interface capacitance. Reported results provide useful guidelines towards further development of WGOFETs and represent a step forward in the understanding of the polymer/water interface phenomena.

  14. Light-based theranostics using hybrid structures derived from biological and organic materials

    Science.gov (United States)

    Vankayala, Raviraj; Burns, Joshua M.; Mac, Jenny T.; Anvari, Bahman

    2016-09-01

    We have engineered hybrid nanostructures derived from erythrocytes, which can be doped with various near infrared (NIR) organic chromophores, including the FDA-approved indocyanine green (ICG). We refer to these vesicles as NIR erythrocyte-mimicking transducers (NETs), as they are capable of generating heat, reactive oxygen species (ROS) or emit fluorescence light. We present preliminary results that demonstrate the effectiveness of NETs for fluorescence imaging and photodynamic therapeutic destruction of breast cancer cells, upon photo-excitation using NIR light. These hybrid nanostructures present a promising platform with theranostic capability for future biomedical clinical applications.

  15. Neighboring block based disparity vector derivation for multiview compatible 3D-AVC

    Science.gov (United States)

    Kang, Jewon; Chen, Ying; Zhang, Li; Zhao, Xin; Karczewicz, Marta

    2013-09-01

    3D-AVC being developed under Joint Collaborative Team on 3D Video Coding (JCT-3V) significantly outperforms the Multiview Video Coding plus Depth (MVC+D) which simultaneously encodes texture views and depth views with the multiview extension of H.264/AVC (MVC). However, when the 3D-AVC is configured to support multiview compatibility in which texture views are decoded without depth information, the coding performance becomes significantly degraded. The reason is that advanced coding tools incorporated into the 3D-AVC do not perform well due to the lack of a disparity vector converted from the depth information. In this paper, we propose a disparity vector derivation method utilizing only the information of texture views. Motion information of neighboring blocks is used to determine a disparity vector for a macroblock, so that the derived disparity vector is efficiently used for the coding tools in 3D-AVC. The proposed method significantly improves a coding gain of the 3D-AVC in the multiview compatible mode about 20% BD-rate saving in the coded views and 26% BD-rate saving in the synthesized views on average.

  16. Clear genetic distinctiveness between human- and pig-derived Trichuris based on analyses of mitochondrial datasets.

    Directory of Open Access Journals (Sweden)

    Guo-Hua Liu

    Full Text Available The whipworm, Trichuris trichiura, causes trichuriasis in ∼600 million people worldwide, mainly in developing countries. Whipworms also infect other animal hosts, including pigs (T. suis, dogs (T. vulpis and non-human primates, and cause disease in these hosts, which is similar to trichuriasis of humans. Although Trichuris species are considered to be host specific, there has been considerable controversy, over the years, as to whether T. trichiura and T. suis are the same or distinct species. Here, we characterised the entire mitochondrial genomes of human-derived Trichuris and pig-derived Trichuris, compared them and then tested the hypothesis that the parasites from these two host species are genetically distinct in a phylogenetic analysis of the sequence data. Taken together, the findings support the proposal that T. trichiura and T. suis are separate species, consistent with previous data for nuclear ribosomal DNA. Using molecular analytical tools, employing genetic markers defined herein, future work should conduct large-scale studies to establish whether T. trichiura is found in pigs and T. suis in humans in endemic regions.

  17. Clear genetic distinctiveness between human- and pig-derived Trichuris based on analyses of mitochondrial datasets.

    Science.gov (United States)

    Liu, Guo-Hua; Gasser, Robin B; Su, Ang; Nejsum, Peter; Peng, Lifei; Lin, Rui-Qing; Li, Ming-Wei; Xu, Min-Jun; Zhu, Xing-Quan

    2012-01-01

    The whipworm, Trichuris trichiura, causes trichuriasis in ∼600 million people worldwide, mainly in developing countries. Whipworms also infect other animal hosts, including pigs (T. suis), dogs (T. vulpis) and non-human primates, and cause disease in these hosts, which is similar to trichuriasis of humans. Although Trichuris species are considered to be host specific, there has been considerable controversy, over the years, as to whether T. trichiura and T. suis are the same or distinct species. Here, we characterised the entire mitochondrial genomes of human-derived Trichuris and pig-derived Trichuris, compared them and then tested the hypothesis that the parasites from these two host species are genetically distinct in a phylogenetic analysis of the sequence data. Taken together, the findings support the proposal that T. trichiura and T. suis are separate species, consistent with previous data for nuclear ribosomal DNA. Using molecular analytical tools, employing genetic markers defined herein, future work should conduct large-scale studies to establish whether T. trichiura is found in pigs and T. suis in humans in endemic regions.

  18. Evaluation and optimization of chitosan derivatives-based gene delivery system via kidney epithelial cells

    Directory of Open Access Journals (Sweden)

    S. Safari

    2012-06-01

    Full Text Available Purpose: Non-viral vectors have been widely proposed as safer alternatives to viral vectors, and cationic polymers have gained increasing attention because they can form self-assembly with DNA. Chitosan is also considered to be a good candidate for gene delivery systems, since it is already known as a biocompatible, biodegradable, and low toxic material with high cationic potential. However, low solubility and transfection efficiency need to be overcome prior to clinical trial. In this work, we focus on alkyl modified chitosan which might be useful in DNA condensing and efficient gene delivery. Methods: N, N- Diethyl N- Methyl (DEMC and N- Triethyl Chitosan (TEC were synthesized from chitosan polymer. In order to optimize the polymers for gene delivery, we used FITC-dextran (FD. Then the optimized polymer concentrations were used for gene delivery. Fluorescent microscope was used, in order to evaluate the polymers’ efficiency for gene delivery to human embryonic kidney epithelial cells (HEK 293T. Results: This modification increased chitosan’s positive charge, thus these chitosan derivatives spontaneously formed complexes with FD, green fluorescence protein plasmid DNA (pEGFP, red fluorescence protein plasmid DNA (pJred and fluorescent labeled miRNA. Results gained from fluorescent microscope showed that TEC and DEMC were able to transfer FD, DNA and miRNA (micro RNA to HEK cell line. Conclusion: We conclude that these chitosan derivatives present suitable characteristics to be used as non-viral gene delivery vectors to epithelial cells.

  19. Radiocarbon-based assessment of fossil fuel-derived contaminant associations in sediments.

    Science.gov (United States)

    White, Helen K; Reddy, Christopher M; Eglinton, Timothy I

    2008-08-01

    Hydrophobic organic contaminants (HOCs) are associated with natural organic matter (OM) in the environment via mechanisms such as sorption or chemical binding. The latter interactions are difficult to quantitatively constrain, as HOCs can reside in different OM pools outside of conventional analytical windows. Here, we exploited natural abundance variations in radiocarbon (14C) to trace various fossil fuel-derived HOCs (14C-free) within chemically defined fractions of contemporary OM (modern 14C content) in 13 samples including marine and freshwater sediments and one dust and one soil sample. Samples were sequentially treated by solvent extraction followed by saponification. Radiocarbon analysis of the bulk sample and resulting residues was then performed. Fossil fuel-derived HOCs released by these treatments were quantified from an isotope mass balance approach as well as by gas chromatography-mass spectrometry. For the majority of samples (n = 13), 98-100% of the total HOC pool was solvent extractable. Nonextracted HOCs are only significant (29% of total HOC pool)in one sample containing p,p-2,2-bis(chlorophenyl)-1,1,1-trichloroethane and its metabolites. The infrequency of significant incorporation of HOCs into nonextracted OM residues suggests that most HOCs are mobile and bioavailable in the environment and, as such, have a greater potential to exert adverse effects.

  20. Intrinsically antibacterial materials based on polymeric derivatives of eugenol for biomedical applications.

    Science.gov (United States)

    Rojo, Luis; Barcenilla, Jose M; Vázquez, Blanca; González, Ramón; San Román, Julio

    2008-09-01

    Infections are the most common cause of biomaterial implant failure representing a constant challenge to the more widespread application of medical implants. This study reports on the preparation and characterization of novel hydrophilic copolymeric systems provided with antibacterial properties coming from eugenol residues anchored to the macromolecular chains. Thus, high conversion copolymers were prepared from the hydrophilic monomer 2-hydroxyethyl methacrylate (HEMA) and different eugenol monomeric derivatives, eugenyl methacrylate (EgMA) and ethoxyeugenyl methacrylate (EEgMA), by bulk polymerization reaction. Thermal evaluation revealed glass transition temperature values in the range 95-58 degrees C following the order HEMA-co-EgMA > PHEMA > HEMA-co-EEgMA and a clear increase in thermal stability with the presence of any eugenyl monomer in the system. In vitro wettability studies showed a reduction of water sorption capacity and surface free energy values with increasing the content of eugenol residues in the copolymer. The antimicrobial activity of copolymeric discs was evaluated by determining their capacity to reduce or inhibit colony formation by different bacterial species. All eugenyl containing materials showed bacteria growth inhibition, this one being higher for the EEgMA derivative copolymers.

  1. NHN-tautomerism in Schiff base derived from pyridoxal 5‧-phosphate and isoniazid: Could it exist?

    Science.gov (United States)

    Gamov, G. A.; Aleksandriiskii, V. V.; Sharnin, V. A.

    2017-02-01

    Structures of Schiff base derived from pyridoxal 5‧-phosphate and isoniazid differing by the quantity of accepted/donated protons were studied by means of quantum chemical calculations (DFT). Based on the total energy and Gibbs energy values the novel intramolecular tautomeric equilibrium consisting in the proton transfer within NHN-group was proposed. The geometry of transition structures was optimized and the values of Gibbs energy of activation of this intramolecular hydrogen transfer were estimated. In the aqueous solution at pH 1 two different NHN-tautomers of Schiff base were found to coexist basing on the comparison of the calculated (TD-SCF) and experimental UV-Vis spectra.

  2. Synthesis, spectroscopic properties and theoretical studies of bis-Schiff bases derived from polyamine and pyrazolones.

    Science.gov (United States)

    Ren, Tiegang; Liu, Shuyun; Li, Guihui; Zhang, Jinglai; Guo, Jia; Li, Weijie; Yang, Lirong

    2012-11-01

    A series of novel bis-Schiff base were synthesized from 1-aryl-3-methyl-4-benzoyl-5-pyrazolones and diethylenetriamine (or triethylenetetramine) as the starting materials. All of these bis-Schiff bases were characterized by means of NMR, IR, and MS. The UV-vis absorption spectra and fluorescent spectra of these bis-Schiff bases were also measured. Moreover, the B3LYP/6-31G(d) method was used to optimize the ground state geometry of the bis-Schiff bases; and the UV-vis spectroscopic properties of the products were computed and compared with corresponding experimental data based on cc-pVDZ basis set of TD-B3LYP method. It has been found that all of these bis-Schiff bases show a remarkable absorption peak in a wavelength range of 270-340 nm; and their maximum emission peaks are around 348 nm.

  3. A green analytical method for the simultaneous analysis of cefixime trihydrate and ambroxol HCl based on ultraviolet derivative spectroscopy

    Directory of Open Access Journals (Sweden)

    Ceema Mathew

    2015-01-01

    Full Text Available Context: Until date, there is no reported derivative spectrophotometric method for the combination of cefixime trihydrate (CEF and ambroxol HCl (ABH. So an urgent need was felt to develop an ultraviolet (UV derivative spectroscopic method, which reduces the cost of analysis on comparing with high-performance liquid chromatography or high-performance thin layer chromatography method. Aims: To develop and validate an economical and ecofriendly derivative spectroscopic method that avoids the use of organic solvents for simultaneous quantification of both the drugs. Materials and Methods: A simple method based on the derivative spectrophotometric method at zero crossing wavelengths has been developed for the simultaneous quantification of CEF and ABH. As the method depends on hydrotropic dissolution, 0.1N urea is used as the solvent, and it yields an economical and ecofriendly method. Two wavelengths 253 nm (zero crossing point (ZCP for CEF and 306 nm (ZCP for ABH were selected for the quantification of ABH and CEF respectively. Results: The first derivative amplitude-concentration plots were linear over the range of 5-35 μg/ml and 3-10.5 μg/ml with detection limits of 0.187 and 0.0937 μg/ml and quantification limits of 0.625 and 0.312 μg/ml for CEF and ABH respectively. The percentage recovery was within the range between 99.05% and 102%. The % relative standard deviation for precision and accuracy of the method was found to be <2%. Conclusion: The proposed method was found to be simple, accurate and precise and can be successfully applied to the routine quality control analysis of studied drugs in their tablet formulations.

  4. Design and synthesis of Quinazolinone, Benzothiazole derivatives bearing guanidinopropanoic acid moiety and their Schiff bases as cytotoxic and antimicrobial agents

    Directory of Open Access Journals (Sweden)

    Palani Venkatesh

    2016-09-01

    Full Text Available Two series of Benzothiazole, Quinazolinone derivatives bearing guanidinopropanoic acid (38 compounds including 27 intermediates and one series of Schiff base derivatives (14 compounds were synthesized, characterized then evaluated for their cytotoxicity against human cervix cell line (HeLa by MTT assay; antimicrobial activity against 11 pathogenic bacteria, 10 pathogenic fungus using standard of ciprofloxacin and Clotrimazole respectively. Compounds 13–18 showed significant activity against HeLa with IC50 range of 2–550 μM. Compound 3-(3-(6-hydroxybenzo[d]thiazol-2-ylguanidinopropanoic acid (18 showed potent activity against human HeLa cell line with the half maximal inhibitory concentration (IC50 values of 1.8 μM which was close to the value of the positive control, doxorubicin. Antimicrobial result indicated that, compounds showed differential activity against the tested fungus and bacteria. Compounds 11, 14, 38 and 49 exhibited potent antibacterial and antifungal activity.

  5. Comparison of consumer derived evidence with an omaha system evidence-based practice guideline for community dwelling older adults.

    Science.gov (United States)

    Pruinelli, Lisiane; Fu, Helen; Monsen, Karen A; Westra, Bonnie L

    2014-01-01

    Consumer involvement in healthcare is critical to support continuity of care for consumers to manage their health while transitioning from one care setting to another. Validation of evidence-based practice (EBP) guideline by consumers is essential to achieving consumer health goals over time that is consistent with their needs and preferences. The purpose of this study was to compare an Omaha System EBP guideline for community dwelling older adults with consumer-derived evidence of their ongoing needs, resources, and strategies after home care discharge. All identified problems were relevant for all patients except for Neglect and Substance use. Ten additional problems were identified from the interviews, five of which affected at least 10% of the participants. Consumer derived evidence both validated and expanded EBP guidelines; thus further emphasizing the importance of consumer involvement in the delivery of home healthcare.

  6. Derivation of airfoil characteristics for the LM 19.1 blade based on 3D CFD rotor calculations

    Energy Technology Data Exchange (ETDEWEB)

    Bak, C.; Soerensen, N.N.; Madsen, H.A. [Risoe National Lab., Roskilde (Denmark)

    1999-03-01

    Airfoil characteristics for the LM 19.1 blade are derived from 3D CFD computations on a full-scale 41-m rotor. Based on 3D CFD the force distributions on the blades are determined, from which airfoil characteristics are derived using the momentum theory. The final airfoil characteristics are constructed using both wind tunnel measurements and 3D CFD. Compared to 2D wind tunnel measurements they show a low lift in stall for the airfoil sections at the tip. At the airfoil sections at the inner part of the blade, they show a high lift in stall. At about 60% radius the lift agrees well to 2D wind tunnel measurements. Aero-elastic calculations using the final airfoil characteristics show good agreement to measured power and flap moments. Furthermore, a fatigue load analysis shows a reduction of up to 15% of the load compared to commonly used data. (au)

  7. Predictive Modeling of Antioxidant Coumarin Derivatives Using Multiple Approaches: Descriptor-Based QSAR, 3D-Pharmacophore Mapping, and HQSAR

    Directory of Open Access Journals (Sweden)

    Indrani MITRA

    2016-09-01

    Full Text Available The inability of the systemic antioxidants to alleviate the exacerbation of free radical formation from metabolic outputs and environmental pollutants claims an urgent demand for the identification and design of new chemical entities with potent antioxidant activity. In the present work, different QSAR approaches have been utilized for identifying the essential structural attributes imparting a potential antioxidant activity profile of the coumarin derivatives. The descriptor-based QSAR model provides a quantitative outline regarding the structural prerequisites of the molecules, while 3D pharmacophore and HQSAR models emphasize the favourable spatial arrangement of the various chemical features and the crucial molecular fragments, respectively. All the models infer that the fused benzene ring and the oxygen atom of the pyran ring constituting the parent coumarin nucleus capture the prime pharmacophoric features, imparting superior antioxidant activity to the molecules. The developed models may serve as indispensable query tools for screening untested molecules belonging to the class of coumarin derivatives.

  8. ECG-Derived Respiration and Instantaneous Frequency based on the Synchrosqueezing Transform: Application to Patients with Atrial Fibrillation

    CERN Document Server

    Yi-Hsin, Chan; Shu-Shya, Hseu; Chi-Tai, Kuo; Yung-Hsin, Yeh

    2011-01-01

    The acquisition of information about respiratory patterns without directly recording the respiratory signals would be beneficial in many clinical settings. The electrocardiogram (ECG)-derived respiration (EDR) algorithm, which derives the respiratory pattern by using the information encoded in ECG signals, enables data acquisition in this manner. However, the traditional EDR algorithm cannot be used in patients with atrial fibrillation (AF) because they have highly irregular heart rates. In this paper, we first provide a definition of ideal instantaneous frequency (IIF) of respiratory signals and then describe how a novel time-frequency representation technique referred to as the Synchrosqueezing transform (SST) was used for the accurate estimation of the IIF of respiratory signals, i.e., SST-IF. Then, we introduce a new EDR algorithm based on the evaluation of the SST-IF. We tested the applicability of our new EDR algorithm in patients with comorbid cardiovascular diseases, most of which were complicated by ...

  9. A novel {sup 125}I-labeled daunorubicin derivative for radionuclide-based cancer therapy

    Energy Technology Data Exchange (ETDEWEB)

    Ickenstein, Ludger M. [Department of Physical and Analytical Chemistry, Uppsala University, P.O. Box 579, 75121 Uppsala (Sweden); Edwards, Katarina [Department of Physical and Analytical Chemistry, Uppsala University, P.O. Box 579, 75121 Uppsala (Sweden); Sjoeberg, Stefan [Department of Biochemistry and Organic Chemistry, Uppsala University, P.O. Box 576, 75123 Uppsala (Sweden); Carlsson, Joergen [Rudbeck Laboratory, Division of Biomedical Radiation Sciences, Department of Oncology, Radiology and Clinical Immunology, Uppsala University, 75185 Uppsala (Sweden); Gedda, Lars [Rudbeck Laboratory, Division of Biomedical Radiation Sciences, Department of Oncology, Radiology and Clinical Immunology, Uppsala University, 75185 Uppsala (Sweden) and Rudbeck Laboratory, Division of Experimental Urology, Department of Surgery, Uppsala University, 75185 Uppsala (Sweden)]. E-mail: lars.gedda@bms.uu.se

    2006-08-15

    Introduction: Auger electron emitters, such as {sup 125}I, are getting increasingly wider recognition as alternatives to current anticancer treatments. The effectiveness of Auger electrons is strongly dependent on their proximity to DNA and is therefore considered as harmless outside the nucleus. Methods: {sup 125}I or {sup 127}I was conjugated with Comp1, Comp2 or Comp3 - three derivatives of the chemotherapeutic drug daunorubicin. Their capacity factors, DNA-binding constants and exclusion parameters, and the degree of DNA fragmentation after incubating isolated DNA with our {sup 127}I- or {sup 125}I-conjugated daunorubicin derivatives were determined. Human breast adenocarcinoma (SK-BR-3) cells were incubated with the derivatives; fluorescent microscopy and autoradiography images were generated; and cell growth was monitored. Results and Discussion: The capacity factor of {sup 127}I-Comp1 was similar to those of daunorubicin and doxorubicin, whereas lower capacity factors of {sup 127}I-Comp2 and {sup 127}I-Comp3 suggested reduced interactions with lipid membranes. DNA exclusion parameters and binding constants of {sup 127}I-Comp1 and {sup 127}I-Comp2, but not of {sup 127}I-Comp3, were similar to those of doxorubicin. Fluorescent microscopy and autoradiography images of SK-BR-3 cells revealed that {sup 127}I-Comp1 and {sup 125}I-Comp1 accumulated in tumor cell nuclei, whereas {sup 127}I-Comp2 and {sup 127}I-Comp3 were present predominantly in other cell compartments. The binding of {sup 125}I-Comp1 to isolated chromosomal DNA led to major fragmentation. Incubation of SK-BR-3 cells with {sup 125}I-Comp1 inhibited cell growth, whereas doxorubicin or {sup 127}I-Comp1 administered at the same concentration had no effect on cell growth. Our results thus suggest that {sup 125}I-Comp1 has the potential to become a new tool for anticancer therapy.

  10. Clear genetic distinctiveness between human- and pig-derived Trichuris based on analyses of mitochondrial datasets

    DEFF Research Database (Denmark)

    Liu, Guo-Hua; Gasser, Robin B.; Su, Ang;

    2012-01-01

    The whipworm, Trichuris trichiura, causes trichuriasis in ~600 million people worldwide, mainly in developing countries. Whipworms also infect other animal hosts, including pigs (T. suis), dogs (T. vulpis) and non-human primates, and cause disease in these hosts, which is similar to trichuriasis...... of humans. Although Trichuris species are considered to be host specific, there has been considerable controversy, over the years, as to whether T. trichiura and T. suis are the same or distinct species. Here, we characterised the entire mitochondrial genomes of human-derived Trichuris and pig...... for nuclear ribosomal DNA. Using molecular analytical tools, employing genetic markers defined herein, future work should conduct large-scale studies to establish whether T. trichiura is found in pigs and T. suis in humans in endemic regions....

  11. New Eugenol Glucoside-based Derivative Shows Fungistatic and Fungicidal Activity against Opportunistic Candida glabrata.

    Science.gov (United States)

    de Souza, Thiago Belarmino; Brito, Keila Mercês de Oliveira; Silva, Naiara Chaves; Rocha, Raissa Prado; de Sousa, Grasiely Faria; Duarte, Lucienir Pains; Coelho, Luiz Felipe Leomil; Dias, Amanda Latércia Tranches; Veloso, Marcia Paranho; Carvalho, Diogo Teixeira; Dias, Danielle Ferreira

    2016-01-01

    A new series of glucosides modified in their saccharide units were synthesized, evaluated against Candida sp., and compared to prototype 1, an eugenol tetracetyl glucoside previously synthesized and shown to be active against Candida glabrata. Among the new glucosides, benzyl derivative 5 was the most promising, showing fungistatic activity at IC50 18.1 μm against Candida glabrata (threefold higher than fluconazole) and fungicidal activity with a low IC90 value of 36.2 μm. Moreover, the cytotoxic activity of compound 5 (CC50 : 580.9 μm), tested in peripheral blood mononuclear cells, suggests its potential as an agent to treat Candida glabrata infections, with a selectivity index of 32. The new eugenol glucoside 5 may be considered as a novel structural pattern in the development of new anti-Candida drugs.

  12. DERIVATIVE OF SET MEASURE FUNCTIONS AND ITS APPLICATION (THEORETICAL BASES OF INVESTMENT OBJECTIVES

    Directory of Open Access Journals (Sweden)

    A. A. Bosov

    2014-04-01

    Full Text Available Purpose. It is necessary to develop the theoretical fundamentals for solving the investment objectives presented in the form of set function as vector optimization tasks or tasks of constrained extremum. Methodology. Set functions and their derivatives of measure are used as research of investment objectives. Necessary condition of set function minimum is proved. In the tasks for constrained extremum the method of Lagrange is used. It is shown that this method can also be used for the set function. It is used the measure for proof, which generalizes the Lebesgue measure, and the concept of set sequence limit is introduced. It is noted that the introduced limit over a measure coincides with the classical Borel limit and can be used in order to prove the existence of derivative from set function over a measure on convergent of sets sequence. Findings. An algorithm of solving the investment objective for constrained extremum in relation to investment objectives was offered. Originality. Scientific novelty lies in the fact that in multivariate objects for constrained extremum one can refuse from immediate enumeration. One can use the proposed algorithm of constructing (selection of options that allow building a convex linear envelope of Pareto solutions. This envelope will let the person who makes a decision (DM, select those options that are "better" from a position of DM, and consider some of the criteria, the formalization of which are difficult or can not be described in mathematical terms. Practical value. Results of the study provide the necessary theoretical substantiation of decision-making in investment objectives, when there is a significant number of an investment objects and immediate enumeration of options is very difficult on time costs even for modern computing techniques.

  13. Development of recombinant collagen-peptide-based vehicles for delivery of adipose-derived stromal cells.

    Science.gov (United States)

    Parvizi, Mojtaba; Plantinga, Josée A; van Speuwel-Goossens, Carolina A F M; van Dongen, Elisabeth M W M; Kluijtmans, Sebastiaan G J M; Harmsen, Martin C

    2016-02-01

    Stem cell therapy is a promising approach for repair, remodeling and even regenerate tissue of otherwise irreparable damage, such as after myocardial infarction (aMI). A severe limitation of cardiac stem cell therapy is the generally poor retention of administered cells in the target tissue. In tissue repair the main mode of action of adipose tissue-derived stem cells (ADSC) is the production of various growth factors, cytokines, anti-inflammatory and anti-apoptotic factors that together augment repair, remodeling, and regeneration. In this experiment, we used recombinant collagen peptide (RCP) with additional integrin-binding motives and different crosslinkers. Formulated as 50-100 μm microspheres with bound ADSC, we hypothesized that this would improve ADSC retention and function. Crosslinking was performed with chemical crosslinkers (EDC and HMDIC) at high and low concentrations or by thermal treatment (DHT). ADSC adhesion, proliferation, apoptosis/necrosis, and gene expressions in two-dimensional and three-dimensional were analyzed. In addition, the effect of ADSC conditioned medium (ADSC-CM) on proapoptotic/sprouting HUVEC was examined. Our results show that all materials support cell adhesion in short time point, however, EDC-High crosslinker induced ADSC apoptosis/necrosis. Gene expression results revealed lower expression of proinflammatory genes in chemical crosslinked materials, despite EDC-High the proinflammatory genes expressions were similar or higher than TCPS. In addition, cultured ADSC on DHT crosslinked RCP showed a proinflammatory phenotype compared to TCPS. Sprouting assay results confirmed the protective effect of ADSC-CM derived from TCPS and HMDIC-High crosslinked RCP proapoptotic HUVEC. We conclude that ADSC adhere to the materials and maintain their therapeutic profile.

  14. Novel 6FDA-based polyimides derived from sterically hindered Tröger's base diamines: Synthesis and gas permeation properties

    KAUST Repository

    Ghanem, Bader

    2016-04-30

    Two novel Tröger\\'s base-based di-o-substituted diamine monomers were synthesized and used to prepare two intrinsically microporous 6FDA-based polyimides (PIM-PI-TB-1 and PIM-PI-TB-2) with high molecular weight, high thermal stability and excellent solubility in common organic solvents. Compared to previously reported methods for preparing TB-based diamines, which are based on reduction of dimerized nitro-substituted anilines or condensation of phenylenediamine derivatives with dianhydrides, the novel protocol can be used to prepare different functionalized TB-based diamine monomers from a wide variety of aniline derivatives. PIM-PI-TB-1 (made from 6FDA and dibromo-tetramethyl-substituted TB diamine) and PIM-PI-TB-2 (made from 6FDA and tetramethyl-substituted TB diamine) are intrinsically microporous polymers with high BET surface areas of 440 m2/g and 580 m2/g, respectively. Pure-gas permeability coefficients of He, H2, N2, O2, CH4, and CO2 were measured at 35 °C and 2 bar for fresh and 180 days aged films. Both TB-based polyimides exhibited high gas permeability with moderate selectivity. The gas permeability dropped significantly coupled with a moderate increase in selectivity after long-term physical aging of 180 days.

  15. Imaging tumors with peptide-based radioligands

    Energy Technology Data Exchange (ETDEWEB)

    Behr, T. M.; Gotthardt, M.; Barth, A.; Behe, M. [Philipps-University of Marburg, Dept. of Nuclear Medicine, Marburg (Germany)

    2001-06-01

    Regulatory peptides are small, readily diffusable and potent natural substances with a wide spectrum of receptor-mediated actions in humans. High affinity receptors for these peptides are (over)-expressed in many neoplasms, and these receptors may represent, therefore, new molecular targets for cancer diagnosis and therapy. This review aims to give an overview of the peptide-based radiopharmaceuticals which are presently already commercially available or which are in advanced stages of their clinical testing so that their broader availability is anticipated soon. Physiologically, these peptides bind to and act through G protein-coupled receptors in the cell membrane. Historically, somatostatin analogs are the first class of receptor binding peptides having gained clinical application. In {sup 111}In-DTPA-(D-Phe{sup 1})-octreotide is the first and only radio peptide which has obtained regulatory approval in Europe and the United States to date. Extensive clinical studies involving several thousands of patients have shown that the major clinical application of somatostatin receptor scintigraphy is the detection and the staging of gastroenteropancreatic neuroendocrine tumors (carcinoids). In these tumors, octreotide scintigraphy is superior to any other staging method. However, its sensitivity and accuracy in other, more frequent neoplasms is limited. Radiolabeled vasoactive intestinal peptide (VIP) has been shown to visualize the majority of gastrointestinal adenocarcinomas, as well as some neuroendocrine tumors, including insulinomas (the latter being often missed by somatostatin receptor scintigraphy). Due to the outstanding diagnostic accuracy of the pentagastrin test in detecting the presence, persistence, or recurrence of medullary thyroid cancer (MTC), it was postulated the expression of the corresponding (i.e. cholecystokinin (CCK-)-B) receptor type in human MTC. This receptor is also widely expressed on human small-cell lung. Indeed, {sup 111}In-labeled DTPA

  16. Novel acyloxy derivatives of branched mono- and polyol esters of sal fat: multiviscosity grade lubricant base stocks.

    Science.gov (United States)

    Kamalakar, Kotte; Sai Manoj, Gorantla N V T; Prasad, Rachapudi B N; Karuna, Mallampalli S L

    2014-12-10

    Sal fat, a nontraditional seed oil, was chemically modified to obtain base stocks with a wide range of specifications that can replace mineral oil base stocks. Sal fatty acids were enriched to 72.6% unsaturation using urea adduct method and reacted with branched mono alcohol, 2-ethylhexanol (2-EtH), and polyols namely neopentyl glycol (NPG) and trimethylolpropane (TMP) to obtain corresponding esters. The esters were hydroxylated and then acylated using propionic, butyric, and hexanoic anhydrides to obtain corresponding acylated derivatives. The acylated TMP esters exhibited very high viscosities (427.35-471.93 cSt at 40 °C) similar to those of BS 150 mineral oil base stock range, ISO VG 460, while the acylated NPG esters (268.81-318.84 cSt at 40 °C) and 2-EtH esters viscosities (20.94-24.44 cSt at 40 °C) exhibited viscosities in the range of ISO VG 320 and 22 respectively with good viscosity indices. Acylated NPG esters were found suitable for high temperature and acylated 2-ethylhexyl esters for low viscosity grade industrial applications. It was observed that the thermo-oxidative stabilities of all acylated products were found better compared to other vegetable oil based base stocks. Overall, all the sal fat based lubricant base stocks are promising candidates with a wide range of properties, which can replace most of the mineral oil base stocks with appropriate formulations.

  17. Searching for primaries in patients with neuroendocrine tumors (NET of unknown primary and clinically suspected NET: Evaluation of Ga-68 DOTATOC PET/CT and In-111 DTPA octreotide SPECT/CT

    Directory of Open Access Journals (Sweden)

    Schreiter Nils Friedemann

    2014-12-01

    Full Text Available Background. To evaluate the clinical efficacy of In-111 DTPA octreotide SPECT/CT and Ga-68 DOTATOC PET/CT for detection of primary tumors in patients with either neuroendocrine tumor of unknown primary (NETUP or clinically suspected primary NET (SNET.

  18. Gd-EOB-DTPA enhanced MRI of the liver: Correlation of relative hepatic enhancement, relative renal enhancement, and liver to kidneys enhancement ratio with serum hepatic enzyme levels and eGFR

    Energy Technology Data Exchange (ETDEWEB)

    Talakic, Emina; Steiner, Jürgen; Kalmar, Peter; Lutfi, Andre [Division of General Radiology, Department of Radiology, Medical University of Graz, Auenbruggerplatz 9, 8036 Graz (Austria); Quehenberger, Franz [Institute for Medical Informatics, Statistics and Documentation, Medical University of Graz, Auenbruggerplatz 2, 8036 Graz (Austria); Reiter, Ursula; Fuchsjäger, Michael [Division of General Radiology, Department of Radiology, Medical University of Graz, Auenbruggerplatz 9, 8036 Graz (Austria); Schöllnast, Helmut, E-mail: helmut.schoellnast@medunigraz.at [Division of General Radiology, Department of Radiology, Medical University of Graz, Auenbruggerplatz 9, 8036 Graz (Austria)

    2014-04-15

    Objectives: To assess the correlation of relative hepatic enhancement (RHE), relative renal enhancement (RRE) and liver to kidneys enhancement ratio (LKR) with serum hepatic enzyme levels and eGFR in Gd-EOB-DTPA enhanced MRI of the liver and to assess threshold levels for predicting enhancement of the liver parenchyma. Methods: Data of 75 patients who underwent Gd-EOB-DTPA enhanced MRI of the liver were collected. Images were obtained before contrast injection, during the early arterial phase, late arterial phase, venous phase, delayed phase, and hepatobiliary phase which was 20 min after Gd-EOB-DTPA administration. Signal intensity of the liver and the kidneys in all phases was defined using region-of-interest measurements for relative enhancement calculation. Serum hepatic enzyme levels and eGFR were available in all patients. Spearman correlation test was used to test the correlation of RHE, RRE and LKR with serum hepatic enzyme levels and eGFR. Results: In the hepatobiliary phase all serum hepatic enzymes were significantly correlated with RHE; total bilirubin (TBIL) and cholin esterase (CHE) showed strongest correlations. TBIL and CHE were significantly correlated with RRE in the arterial phases. TBIL and CHE were significantly correlated with LKR in the arterial phase and hepatobiliary phase. eGFR showed no correlation. Conclusions: In Gd-EOB-DTPA enhanced MRI, TBIL and CHE levels may predict RHE, RRE and LKR.

  19. Natural-product-based insecticidal agents 14. Semisynthesis and insecticidal activity of new piperine-based hydrazone derivatives against Mythimna separata Walker in vivo.

    Science.gov (United States)

    Qu, Huan; Yu, Xiang; Zhi, Xiaoyan; Lv, Min; Xu, Hui

    2013-10-15

    In continuation of our program aimed at the discovery and development of natural-product-based insecticidal agents, twenty-six new piperine-based hydrazone derivatives were synthesized from piperine, an alkaloid isolated from Piper nigrum Linn. The single-crystal structures of 6c, 6q and 6w were unambiguously confirmed by X-ray crystallography. Their insecticidal activity was evaluated against the pre-third-instar larvae of Mythimna separata Walker in vivo. Especially compounds 6b, 6i and 6r, the final mortality rates of which, at the concentration of 1 mg/mL, were 62.1%, 65.5% and 65.5%, respectively, exhibited more pronounced insecticidal activity compared to toosendanin at 1 mg/mL, a commercial botanical insecticide isolated from Melia azedarach. It suggested that introduction of the substituents at the C-2 position on the phenyl ring of the hydrazone derivatives was important for their insecticidal activity.

  20. Deriving statistical significance maps for SVM based image classification and group comparisons

    OpenAIRE

    Gaonkar, Bilwaj; Davatzikos, Christos

    2012-01-01

    Population based pattern analysis and classification for quantifying structural and functional differences between diverse groups has been shown to be a powerful tool for the study of a number of diseases, and is quite commonly used especially in neuroimaging. The alternative to these pattern analysis methods, namely mass univariate methods such as voxel based analysis and all related methods, cannot detect multivariate patterns associated with group differences, and are not particularly suit...

  1. Synthesis, characterization and antibacterial activity of a tridentate Schiff base derived from cephalothin and sulfadiazine, and its transition metal complexes.

    Science.gov (United States)

    Anacona, J R; Noriega, Natiana; Camus, Juan

    2015-02-25

    Metal(II) coordination compounds of a cephalothin Schiff base (H2L) derived from the condensation of cephalothin antibiotic with sulfadiazine were synthesized. The Schiff base ligand, mononuclear [ML(H2O)3] (M(II)=Mn,Co,Ni,Zn) complexes and magnetically diluted dinuclear copper(II) complex [CuL(H2O)3]2 were characterized by several techniques, including elemental and thermal analysis, molar conductance and magnetic susceptibility measurements, electronic, FT-IR, EPR and (1)H NMR spectral studies. The cephalothin Schiff base ligand H2L behaves as a dianionic tridentate NOO chelating agent. The biological applications of complexes have been studied on two bacteria strains (Escherichia coli and Staphylococcus aureus) by agar diffusion disc method.

  2. Gelatin-Based Hydrogels Promote Chondrogenic Differentiation of Human Adipose Tissue-Derived Mesenchymal Stem Cells In Vitro

    Directory of Open Access Journals (Sweden)

    Achim Salamon

    2014-02-01

    Full Text Available Due to the weak regeneration potential of cartilage, there is a high clinical incidence of articular joint disease, leading to a strong demand for cartilaginous tissue surrogates. The aim of this study was to evaluate a gelatin-based hydrogel for its suitability to support chondrogenic differentiation of human mesenchymal stem cells. Gelatin-based hydrogels are biodegradable, show high biocompatibility, and offer possibilities to introduce functional groups and/or ligands. In order to prove their chondrogenesis-supporting potential, a hydrogel film was developed and compared with standard cell culture polystyrene regarding the differentiation behavior of human mesenchymal stem cells. Cellular basis for this study were human adipose tissue-derived mesenchymal stem cells, which exhibit differentiation potential along the adipogenic, osteogenic and chondrogenic lineage. The results obtained show a promotive effect of gelatin-based hydrogels on chondrogenic differentiation of mesenchymal stem cells in vitro and therefore encourage subsequent in vivo studies.

  3. Synthesis, characterization and antibacterial activity of a Schiff base derived from cephalexin and sulphathiazole and its transition metal complexes.

    Science.gov (United States)

    Anacona, J R; Rodriguez, Juan Luis; Camus, Juan

    2014-08-14

    Metal(II) coordination compounds of a cephalexin Schiff base (HL) derived from the condensation of cephalexin antibiotic with sulphathiazole were synthesized. The Schiff base ligand, mononuclear [ML(OAc)(H2O)2] (M(II)=Mn, Co, Ni, Zn) complexes and magnetically diluted trinuclear copper(II) complex [Cu3L(OH)5] were characterized by several techniques, including elemental and thermal analysis, molar conductance and magnetic susceptibility measurements, electronic, FT-IR, EPR and (1)H NMR spectral studies. The analytical and molar conductance values indicated that the acetate ions coordinate to the metal ions. The Schiff base ligand HL behaves as a monoanionic tridentate NNO and tetradentate NNOO chelating agent in the mono and trinuclear complexes respectively.

  4. Synthesis, characterization and antibacterial activity of a Schiff base derived from cephalexin and sulphathiazole and its transition metal complexes

    Science.gov (United States)

    Anacona, J. R.; Rodriguez, Juan Luis; Camus, Juan

    2014-08-01

    Metal(II) coordination compounds of a cephalexin Schiff base (HL) derived from the condensation of cephalexin antibiotic with sulphathiazole were synthesized. The Schiff base ligand, mononuclear [ML(OAc)(H2O)2] (M(II) = Mn, Co, Ni, Zn) complexes and magnetically diluted trinuclear copper(II) complex [Cu3L(OH)5] were characterized by several techniques, including elemental and thermal analysis, molar conductance and magnetic susceptibility measurements, electronic, FT-IR, EPR and 1H NMR spectral studies. The analytical and molar conductance values indicated that the acetate ions coordinate to the metal ions. The Schiff base ligand HL behaves as a monoanionic tridentate NNO and tetradentate NNOO chelating agent in the mono and trinuclear complexes respectively.

  5. Assessment of Gradient-Based Similarity Functions in the Stable Boundary Layer Derived from a Large-Eddy Simulation

    Science.gov (United States)

    Sorbjan, Zbigniew

    2017-02-01

    Gradient-based similarity functions, evaluated based on data generated by a large-eddy simulation model of the stably stratified boundary layer, are compared with analogous similarity functions, derived from field observations in the surface layer during the Surface Heat Budget of the Arctic Ocean (SHEBA) experiment in the Arctic. The comparison is performed in terms of explicit and implicit local scaling systems, for the temperature and momentum fluxes, standard deviations of the vertical velocity and of temperature, as well as dissipation rates for the turbulent kinetic energy and for the temperature variance. The comparison shows the best agreement of the SHEBA-based similarity functions with analogous functions evaluated using the large-eddy simulation data in the range of the Richardson number 0.01<{ Ri}< 0.1.

  6. Synthesis, characterization and antibacterial activity of a tridentate Schiff base derived from cephalothin and sulfadiazine, and its transition metal complexes

    Science.gov (United States)

    Anacona, J. R.; Noriega, Natiana; Camus, Juan

    2015-02-01

    Metal(II) coordination compounds of a cephalothin Schiff base (H2L) derived from the condensation of cephalothin antibiotic with sulfadiazine were synthesized. The Schiff base ligand, mononuclear [ML(H2O)3] (M(II) = Mn, Co, Ni, Zn) complexes and magnetically diluted dinuclear copper(II) complex [CuL(H2O)3]2 were characterized by several techniques, including elemental and thermal analysis, molar conductance and magnetic susceptibility measurements, electronic, FT-IR, EPR and 1H NMR spectral studies. The cephalothin Schiff base ligand H2L behaves as a dianionic tridentate NOO chelating agent. The biological applications of complexes have been studied on two bacteria strains (Escherichia coli and Staphylococcus aureus) by agar diffusion disc method.

  7. Microplate assay for screening the antibacterial activity of Schiff bases derived from substituted benzopyran-4-one.

    Science.gov (United States)

    Amin, Rehab M; Abdel-Kader, Nora S; El-Ansary, Aida L

    2012-09-01

    Schiff bases (SB(1)-SB(3)) were synthesized from the condensation of 6-formyl-7-hydroxy-5-methoxy-2-methylbenzopyran-4-one with 2-aminopyridine (SB(1)), p-phenylenediamine (SB(2)) and o-phenylenediamine (SB(3)), while Schiff bases (SB(4)-SB(6)) were synthesized by condensation of 5,7-dihydroxy-6-formyl-2-methylbenzopyran-4-one with 2-aminopyridine (SB(4)), p-phenylenediamine (SB(5)) and o-phenylenediamine (SB(6)). Schiff bases were characterized using elemental analysis, IR, UV-Vis, (1)H NMR, (13)C NMR and mass spectroscopy. These compounds were screened for antibacterial activities by micro-plate assay technique. Escherichia coli and Staphylococcus capitis were exposed to different concentrations of the Schiff bases. Results showed that the antibacterial effect of these Schiff bases on Gram-negative bacteria were higher than that on Gram-positive bacteria moreover, the Schiff bases containing substituent OCH(3) on position five have higher antibacterial activity than that containing hydroxy group on the same position.

  8. ¹¹¹In-Bn-DTPA-nimotuzumab with/without modification with nuclear translocation sequence (NLS) peptides: an Auger electron-emitting radioimmunotherapeutic agent for EGFR-positive and trastuzumab (Herceptin)-resistant breast cancer.

    Science.gov (United States)

    Fasih, Aisha; Fonge, Humphrey; Cai, Zhongli; Leyton, Jeffrey V; Tikhomirov, Ilia; Done, Susan J; Reilly, Raymond M

    2012-08-01

    Increased expression of epidermal growth factor receptors (EGFR) in breast cancer (BC) is often associated with trastuzumab (Herceptin)-resistant forms of the disease and represents an attractive target for novel therapies. Nimotuzumab is a humanized IgG(1) monoclonal antibody that is in clinical trials for treatment of EGFR-overexpressing malignancies. We show here that nimotuzumab derivatized with benzylisothiocyanate diethylenetriaminepentaacetic acid for labelling with the subcellular range Auger electron-emitter, (111)In and modified with nuclear translocation sequence (NLS) peptides ((111)In-NLS-Bn-DTPA-nimotuzumab) was bound, internalized and transported to the nucleus of EGFR-positive BC cells. Emission of Auger electrons in close proximity to the nucleus caused multiple DNA double-strand breaks which diminished the clonogenic survival (CS) of MDA-MB-468 cells that have high EGFR density (2.4 × 10(6) receptors/cell) to less than 3 %. (111)In-Bn-DTPA-nimotuzumab without NLS peptide modification was sevenfold less effect